LOCUS       DASWHX010000001         1832 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_369597, whole genome shotgun sequence.
ACCESSION   DASWHX010000001 DASWHX010000000
VERSION     DASWHX010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 1832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1832
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_369597"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1475)
                     /locus_tag="VGA21_00005"
     CDS             complement(<1..1475)
                     /locus_tag="VGA21_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009186222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9648911.1"
                     /translation="MKSLIYLSAAVFFYGCTAENQPTSPKFEISFSDAMSTESFDGRL
                     LLILSNNDTAEPRFQINDGHNTQLIYGIDVEDYKPGENRVIDAAVFGYPLKSLSEIPE
                     GDYYVQALLHKYETFNLSTGHQVKLPMDQGEGQHWNRSPKNFYSSAQKIHYNPNSEEV
                     INVIMENEIPPIEEPVDTKYIKHIKIQSKLLTEFWGTPMFLGAHVLVPEGFEDHPEAQ
                     YPLMVFHGHYPSDFGGFRTEPPDPNLEPDYSERFRLEGYNIIQQQEAYDFYQKWISAD
                     FPRFLAIEIQHQNPYYDDSYAVNSENLGPYGDAITYELIPEIEKRFRGIGEGWARFLY
                     GGSTGGWEALAVQVKYPKEYNGCFAACPDPIDFRAYTTVNIYEDKNAYYYESAYKELL
                     KPAHRDYLGQINATVQSMNHRELVLGTKSRSGDQFDIWEAVYSPQGSDGYPQRIWDKM
                     TGEINPEVAEFWKENYDLRYILERDWEKLGADLQGKIHIFCG"
BASE COUNT          503 a          400 c          368 g          561 t
ORIGIN      
        1 ccacagaaaa tatggatctt gccttgaaga tccgcaccta gtttttccca atcccgttcc
       61 aggatgtaac gcaaatcgta attctccttc caaaactccg caacttccgg attgatttct
      121 ccggtcattt tatcccaaat ccgttgcggg tagccgtcgg agccttgtgg tgaatacacc
      181 gcttcccaga tatcaaattg atcgcccgac cgggattttg ttcctaaaac caattctctg
      241 tgattcatac tttgcacagt ggcatttatc tgtcccaggt aatcccgatg ggcaggtttt
      301 agcaattcct tataggctga ttcgtaataa tacgcattct tatcttcata gatattcaca
      361 gtagtgtagg ccctgaaatc aattgggtcc ggacaagcgg caaaacagcc gttgtattct
      421 ttcggatatt tcacctgtac agcaagagct tcccagccgc ccgttgaacc cccgtaaagg
      481 aaccttgccc aaccttcgcc aattccccga aagcgttttt caatttccgg aatcagttca
      541 taggtaatgg catccccgta aggcccaagg ttttctgaat tcaccgcata actgtcgtcg
      601 tagtatgggt tttgatgttg aatttctatg gccaaaaatc ttgggaaatc agcgctaatc
      661 catttttgat agaaatcata tgcttcttgt tgttgaataa tgttataacc ttccaaccgg
      721 aagcgttcac tgtaatccgg ctcaagattc ggatcaggag gttcagttct gaaacctcca
      781 aaatcggagg gataatgtcc atgaaatacc atcaacggat attgagcttc cggatgatct
      841 tcaaatcctt caggaaccaa tacatgagca cccagaaaca tcggcgttcc ccagaactct
      901 gtcagaagtt tactttgaat tttaatatgc tttatatatt tggtatctac cggttcttca
      961 atgggtggaa tttcattttc catgattaca ttgataactt cctccgaatt cggattgtaa
     1021 tgaatttttt gcgcggagct ataaaaattc tttggcgacc ggttccaatg ctggccttcg
     1081 ccttgatcca taggtaattt tacctggtga cctgtggaaa gattgaatgt ttcgtattta
     1141 tgcagtaatg cctgcacata ataatctcct tcgggtattt ctgacaaact tttcagagga
     1201 tacccaaaaa cggccgcgtc aattacccga ttttcgcctg gtttgtaatc ttccacatca
     1261 atgccataaa tcagctgtgt gttgtgtcca tcattaattt gaaatctggg ctcggccgtg
     1321 tcattattag acagtattaa taatagccgg ccatcaaacg attctgtcga catggcatca
     1381 gaaaatgaaa tttcaaattt tggtgaagtt ggttggtttt ccgcagtaca accatagaaa
     1441 aataccgcgg ctgataaata aattaagctt ttcatagtgg atgattttag aatagtggtg
     1501 attttagata aacaagttat gaaaaggaat ggaaaagacc agtttcattt actggaattg
     1561 caatactgca tagccttgct ttggactaac agttccagtt agaaggtctg tccagagctt
     1621 gcatctttaa gagctatgac atgcatgacg ctcaatgaaa agggcttgcc ggttgattat
     1681 ggcaagccct tcgtttggtt gagcgttgaa ttaagaatat ctgttcgatc aaaataaatc
     1741 catatcaatg agattctccc cattgttaaa tcttctgcca attgtgttga caacataagt
     1801 gaggacaact gctgcagcaa ctccggccgt ga
//
LOCUS       DASWHX010000002         8663 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_740345, whole genome shotgun sequence.
ACCESSION   DASWHX010000002 DASWHX010000000
VERSION     DASWHX010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 8663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8663
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_740345"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(98..1081)
                     /locus_tag="VGA21_00010"
     CDS             complement(98..1081)
                     /locus_tag="VGA21_00010"
                     /inference="COORDINATES: protein motif:HMM:NF019826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HEX9648912.1"
                     /translation="MKIKDKIIAALKESGDLSVKELVDSLNVSKQAIHVAINQLLEAD
                     AIIKFGRTPKTIYRLHRVKTFVVKDHDITNTETLKYLSKNFLVITELGKMLEGAEAFS
                     LWCKQRNLPMEKTAEEYIKTKSKYDAYFDAKGFINGKEKLTNTKGFSKINLNNIYYLD
                     FYAIERFGKTRLGTLLHYAKQGQNKFLMRIMLSEIKPRVDTLIKTNSFDAVGFVPPTI
                     RREVQIMKYLETHLKINLPRIKIQKISGIIPVPQKSLSKIEERINNAEHTFAVNETVK
                     YKHLLLIDDAVGSGSTMNQIARKIKQKGISKKVTGLAVVGSFKGFDVITDV"
     gene            1670..2173
                     /locus_tag="VGA21_00015"
     CDS             1670..2173
                     /locus_tag="VGA21_00015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9648913.1"
                     /translation="MFKESKFWKTAILAWVIFIGIDFFFHAAVFAQLWSEKVESFKTQ
                     AELFRLIPAGYGSFLLLTILIIFTFNQSFNSTPTLKKSVTFSLVFGLLFSVSYFLGLY
                     SFLRIPVPHLAAFSMVYFIEIFGVCMISHFSKMVSFRKLTLFTLSVFFFLIIIGILIQ
                     NLSGNLP"
     gene            2280..3764
                     /locus_tag="VGA21_00020"
     CDS             2280..3764
                     /locus_tag="VGA21_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018620198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase"
                     /protein_id="HEX9648914.1"
                     /translation="MKLITPFLLFTAIAVHSAAQDRMLEAESAVTTVHEVTIKGQKIS
                     YSATAGTQPVWNQDGKVIASLFYTYYERSGISDRSTRPLVISFNGGPGSASVWMHIAY
                     TGPRVLLIDDEGYPVQPYGVRENPNSILDVADIVYVNPVNTGYSRILDKDTNTSVFFG
                     VNPDIEYLAEWINTFVGRKNRWRSPKYLIGESYGTTRVSGLALELQESHWMFFNGVIL
                     VSPTGLGIERDGPVDAANRIPYFAAAAWYHKRLPSDLQSKDLLEVLPEAENFAINELI
                     PAMAMGGFLTEDKKQEMAAKISRYSGLSEKIVLQHNLDIPTSFFWKELLRDKGFTIGR
                     LDSRYLGIDRQDAGERPDFNSELISWEHSFTPAINYYLRENLNYKTDIKYNVFGSVRP
                     WDREGDQTGENLRKAMAENPYMNVMIQSGYYDGATTYFNAKYTMWNLDPSGKLKDRLR
                     FEGYRSGHMMYLRKEDLASANDHIRDFIRKTIPPKGMPAKYEVK"
     gene            complement(4127..6022)
                     /locus_tag="VGA21_00025"
     CDS             complement(4127..6022)
                     /locus_tag="VGA21_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013926867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="von Willebrand factor type A domain-containing
                     protein"
                     /protein_id="HEX9648915.1"
                     /translation="MKTMMTTILLTALLSMGSVLAQKKMLTGRVIFAYDNSPVEKVSV
                     KNNDSGISTITDKNGDFSLEVEYGNTILFKKKGFENFELVVGNQTHVNILLEKEKGTV
                     TDGKKEETTLNVFRPVEKDKHSLADQRRLSAPVFMESAPSGGYGDVSLNQDINTNFNT
                     EEYDHFQDNIFKTPASAPLSTFSIDVDAASYSNLRRFLLQYGQKPPKDAVRIEEMVNY
                     FTYNYEQPKGEHPFSIITEVAAAPWNENHKLVHIGLQGIKPDYESMQPSNLVFLVDVS
                     GSMSDPNKLPLLISSFKLMVNELRPDDRVAIVVYAGAAGLVLPSTPAKDKQTIISALE
                     RLTAGGSTAGGAGIALAYKVAQENFREGGNNRVILATDGDFNIGTSSTSELVRMIEEK
                     RKSGIYLTIAGFGMGNYKDGRMEQISNAGNGNYFYIDNLSEAKKVFVTELRATLFTIA
                     KDVKIQVEFNPDIVAAYRLIGYENRILNSEDFNDDKKDAGELGAGHTVTALYEIIPVG
                     VESEFIKDVDPLKYQNNNSPVTTSGKELLTVKFRYKKPDEDVSRLIVKTLRDEERSWE
                     KASEDFRWSASVAGFGMLMRDSEFKGKSSYGQVLTIANGAKGVDTEGYRAEFIQMVKS
                     LELIARR"
     gene            6112..6708
                     /locus_tag="VGA21_00030"
     CDS             6112..6708
                     /locus_tag="VGA21_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009195947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX9648916.1"
                     /translation="MTQKERNSNLSDKQLIELFQKTGSTEYLGIVYSRYIHLIYGVCL
                     KYLKERESSRDAVMDIFESLLSKMEGQEIRDFGKWIYVVAKNHCLMRLRKREIVINNE
                     NAGHFIMESAEIMHLNNEETLYNEETLSENKIKKLEAGVDELPLEQKICIRLFYYEEK
                     SYSQIAAETGYDLKKVKSYVQNGKRNLKIWMENKSERK"
     gene            6695..7825
                     /locus_tag="VGA21_00035"
     CDS             6695..7825
                     /locus_tag="VGA21_00035"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEX9648917.1"
                     /translation="MSENKNHIAHLSLSKMKDYLSGKLTEKESHQIEIHMQECNLCAD
                     ALMGMERMDPGIIGEDVSHLQSLVIEKTRARSRTSWKVRIAAAVVILAISSYLVVSYL
                     QLGGKNQIVASEDSGTESDAQAREGDALAMKDSIQEMNKPAAAEDIEIAGRQLPDERD
                     QTFIENEIDTGAGTDEVTIDMKVVQAETEVDMPEKGLAEEVIITEAEPELLADQKLEG
                     VKEELVKEEAIFRRNSKSAAQSIADQPVEAAPELNTLSGFAAKEVLLIIRVIQGNENA
                     DKNAEPESGTIEYVNQILNDLQYPEKASIAEVRGRVTLIFQVGEDGSLSEFKITQSLG
                     YGCDEEAIRVIKKGSRWNAAILAGKPVSQQIEVMVPFPKSGK"
BASE COUNT         2495 a         1715 c         1818 g         2635 t
ORIGIN      
        1 ggttgtcgtt gtggcgatag gagatgaaga tgtcgtattc gtagccgggg actatagatg
       61 gcatatttca aaagatagta aattccaaaa gagtgactta cacatcggta atcacatcaa
      121 agcctttgaa gctacccacc acggcaagac cggtgacttt ttttgagatg cctttctgtt
      181 ttatttttct cgcaatttga ttcattgtag aaccgctgcc aacggcatcg tcaatcaaga
      241 gaaggtgttt gtattttact gtctcattta ccgcgaaggt atgctcagca ttattgattc
      301 gctcttcaat tttgctcagt gatttttgag gtaccggtat gatgcctgat attttctgaa
      361 tctttatcct tggcaggttt atttttagat gtgtttccag gtacttcata atctgaactt
      421 ctcgtctgat ggtaggcggt acaaagccta ccgcatcaaa cgaatttgtc tttatgagag
      481 tatctactct cggtttgatt tcggagagca taattctcat cagaaatttg ttttgccctt
      541 gtttggcgta atgcaatagc gtacccaatc tggtcttgcc gaatcgctca atagcgtaaa
      601 agtccaggta atagatgtta ttcaggttta ttttgctaaa accttttgtg ttggtgagtt
      661 tttcctttcc attgataaaa cctttggcat cgaaataagc atcgtattta ctttttgttt
      721 ttatatactc ttcggctgtt ttttccatgg gtaggttacg ttgtttacac caaagactaa
      781 aagcttctgc gccttccagc attttgccta attcagtgat caccagaaaa ttctttgata
      841 gatattttag ggtttcggta ttggtgatgt catggtcttt gaccacgaaa gtttttaccc
      901 ggtgtagccg gtagatggtt ttgggtgttc tgccaaattt tattatcgca tccgcttcca
      961 gaagttgatt aatcgccacg tgaattgcct gtttggaaac attcaaagaa tctacaagtt
     1021 ctttcacgct caaatcgcct gattctttta gcgcagcaat gattttgtcc ttaattttca
     1081 ttttagacta ttgactatca atagtcaaaa gtaatgtaac tattactgct ttacaacgtc
     1141 aattatggct tttttaattt aattaaggcg atccccaagt taagatgcac agacgttatc
     1201 attctgccct taggagatga ggatgtcgta ttcgtaattg gagataaagg agggcatggt
     1261 tcaaatatac aaatgaaatt gtctttgttg gagaggaatt gggaaggcaa cgtcccgtag
     1321 ggacaataat gatggtagcg gttgggtagt ttgcgcgatg cctgtcccgt tagggacgga
     1381 atgttataaa tcatttgtcc actgaaatgt tgcaactcca atggatatgt atatgctggt
     1441 tatatatatc gtaacgtgtt tataaatttc taggttctgc taccgtctta tgccccgcca
     1501 aagattgtaa ctatcttaag tccatcccat tccggttaat gggtggcgta cctttactat
     1561 ttgcacaaat ccctgaagaa attctctaaa ccaattttaa atattttctt tttcattcct
     1621 gattgaaatc atttctttcc tccggttgaa ggccgaattt catatgtcta tgttcaaaga
     1681 aagtaagttc tggaaaacag caattctggc ctgggttatt tttatcggaa ttgatttctt
     1741 cttccatgcc gctgtatttg cacaattatg gtcagaaaag gtggaatcat tcaaaacaca
     1801 ggcagagctt ttcaggctaa ttcctgcagg atatggtagt ttcttgcttc taaccatcct
     1861 gattattttt accttcaatc aaagttttaa ttctactcct accttgaaga aaagcgttac
     1921 cttttctctt gttttcgggt tgttgttttc agtgagctat tttcttggtc tttattcatt
     1981 cctaagaata cccgtcccac acctggcagc tttcagtatg gtttatttta ttgaaatttt
     2041 tggagtttgc atgatttcac atttctcgaa aatggtctca ttcaggaaat tgacattatt
     2101 cacactctct gttttcttct tcctgattat tattggtatt ctaattcaaa atttatcagg
     2161 aaatctcccg taaaaacctc ccgtatattt attcttgccg ggctgaattc taatgtttac
     2221 attcacagtt attgctaaaa attagttccc aaaaaaatct aactaaaaac taaattccca
     2281 tgaaattaat tacccctttt ttgctattca ctgcaattgc agtgcattca gcagcccagg
     2341 acaggatgct ggaagctgaa tctgccgtta ccactgtcca tgaagtgacc atcaaaggtc
     2401 aaaagatttc ttacagtgca accgccggca cccagcctgt ttggaatcag gatgggaaag
     2461 taattgcgtc acttttctat acgtattatg aaagaagcgg tatcagtgat cgtagtaccc
     2521 ggcccctggt aatttctttt aatggcggtc ccggatcagc ttccgtctgg atgcatattg
     2581 cttacacagg tccaagggtt ttgctaatcg atgatgaagg ttacccggtt cagccttatg
     2641 gcgtgcggga aaatcccaac tctattctgg atgttgccga tattgtatat gttaatccgg
     2701 tgaacaccgg gtacagcaga attttggata aggatacgaa cacttccgta ttttttggcg
     2761 taaacccgga tatagaatac ctggccgaat ggataaatac ttttgttggc cggaaaaatc
     2821 gttggcgctc acccaaatat ctgattggcg aaagctatgg aaccacgaga gtttcaggct
     2881 tggctttgga acttcaggaa tcacattgga tgttctttaa cggcgtaatt ctggtttctc
     2941 caacgggatt aggcatagaa agagatggtc cggttgatgc cgccaacagg attccttatt
     3001 ttgcagcagc tgcatggtat cataaaaggt taccctcaga tcttcagtca aaagatttgc
     3061 ttgaagttct gcctgaagct gagaatttcg cgattaacga acttattcca gctatggcta
     3121 tgggagggtt tcttactgaa gataaaaaac aggaaatggc tgcaaaaata tccaggtatt
     3181 cagggttatc agaaaagata gttctccagc acaatcttga cattccaact tcatttttct
     3241 ggaaagaatt acttcgtgat aaaggattta caatcggtcg actggattcc aggtatttgg
     3301 gaatagatcg ccaggatgcg ggagaaagac ccgatttcaa ttcagaatta atttcgtggg
     3361 aacactcatt tacccctgct ataaattatt atctgagaga gaacctgaat tataaaaccg
     3421 acattaaata taatgtgttc ggatctgttc gtccatggga tagggaaggc gatcaaacgg
     3481 gagaaaacct taggaaagcc atggcggaaa atccctatat gaatgtaatg atccagtccg
     3541 gatattacga tggcgcgact acctacttta atgcaaaata tacgatgtgg aacctggacc
     3601 ccagcgggaa gctcaaagac aggcttcggt ttgaaggcta tcgcagcggg catatgatgt
     3661 atttacgcaa agaagattta gcatcagcca atgatcatat aagagatttc ataaggaaga
     3721 cgattcctcc aaaagggatg ccggctaaat atgaagtgaa gtaatatctg cggctaaaaa
     3781 tttcattatg tcgaaaatct gagaaaccgg atacccgaat tagtatgttc atttatataa
     3841 aacccttttc aggggtatgg gtctgcaccg tacacttcta ttgttcacat ctgtacagat
     3901 taaatttcca ttcacgtaca aatcaactct aaactactga ttttcaaaag tttaattttt
     3961 ttggcatagc cattggaaat aaacaggtat ctcaaattaa atagcgataa cagctaagat
     4021 tcgtccccgg cataccgggg cgaatcttac aactcggatt tacaaggtta ataatgggtt
     4081 aagtaggtag aaaagagggc gggtgccctc ttttcatttt aagtaattac cggcgagcta
     4141 taagttccag acttttaacc atctgaatga actcagctct gtacccttct gtgtccactc
     4201 cttttgcacc attagcaatc gtcagtacct gaccataaga actcttcccc ttaaattccg
     4261 aatcgcgcat cagcatcccg aacccggcaa cagacgccga ccagcggaaa tcttctgaag
     4321 ccttctccca gcttctttct tcatccctaa gtgtttttac gattaacctg ctaacatctt
     4381 catccggttt tttgtatctg aatttaaccg ttagcaattc ctttccggat gtggtaacag
     4441 gtgagttatt gttttgatat tttaaagggt caacatcttt tataaattca gactccactc
     4501 ctaccgggat tatttcatac aaagccgtta cagtgtgtcc ggcgcccagt tctccggcat
     4561 ctttcttatc atcattgaaa tcctcactgt tcaggattct gttttcataa cctattaacc
     4621 tgtacgcggc tacaatgtca ggattaaatt caacctgaat cttaacatcc ttcgcaatgg
     4681 tgaacaaagt agcacggagt tcggtaacaa aaactttctt cgcttcagat aaattgtcaa
     4741 tgtagaagta gtttccattt ccggcattgc taatctgttc cattcttcca tctttataat
     4801 ttcccattcc aaaaccggca atagtcaggt atatgccgct ctttctcttt tcttctatca
     4861 ttcgtaccag ttctgaagtt gaagaggtac caatattgaa gtcgccatca gtggcaagga
     4921 ttaccctgtt gttaccccct tcccggaaat tctcctgcgc tactttgtat gccaacgcta
     4981 ttccggctcc tccggcggtt gatcctccgg ctgtaagtct ttccaaagca ctgattatag
     5041 tttgcttatc tttagcggga gttgatggta acaccaggcc cgcggcccct gcataaacta
     5101 caattgctac ccggtcgtcc ggtcttagtt cattcaccat taatttaaat gaagaaatca
     5161 acagaggaag cttgttcgga tcggacattg atccactgac atccaccagg aaaacaagat
     5221 ttgagggttg catagattca taatctggtt ttattccctg cagtccgata tgaaccagct
     5281 tatgattttc attccatgga gcggcagcca cttcggtgat gatggaaaac ggatgctcac
     5341 ctttgggctg ctcgtaatta taggtaaaat aattcaccat ttcttcaatg cgaactgcat
     5401 cttttggtgg cttctgccca tattgcaata agaagcgacg caggttggag taggatgcgg
     5461 catccacatc aatcgagaat gtcgaaagtg gcgccgaggc gggtgtcttg aaaatgttat
     5521 cctggaaatg atcatattcc tcggtattga aattggtatt tatatcctga ttaagtgaaa
     5581 catctccata tccccctgaa ggagctgatt ccataaaaac cggagctgat aatcttctct
     5641 gatcagcgag tgaatgttta tccttttcca ctggtctgaa tacattcaat gttgtctctt
     5701 cttttttccc atcagtcacg gttccctttt ccttttccaa aagaatgtta acatgcgttt
     5761 ggttgccaac tacaagttca aagttttcaa accccttctt tttgaaaaga atagtgttgc
     5821 catattcaac ttctaaggaa aaatcaccat tcttatcagt aatagtagaa ataccagaat
     5881 cattattttt taccgatact ttttcaaccg gagaattatc ataggcaaat attaccctgc
     5941 ctgtcagcat cttcttttgt gccaacaccg accccatact taataatgcg gtcaataaaa
     6001 ttgtggtcat catcgttttc attttcagta tatgttttct taaaggatgc cgagcggtgg
     6061 aaaattccac aaatagtttg cttaatttta cgtttcatgt tgtaagttct tatgactcag
     6121 aaggaaagaa atagcaacct gtctgacaag caattgattg aattgtttca aaaaaccggt
     6181 agcactgaat atttaggaat tgtttatagc cgttatattc acttgatcta tggtgtttgc
     6241 ctgaaatatt tgaaagaaag agagtcttcc cgggatgctg ttatggatat ttttgaaagt
     6301 cttctttcga agatggaagg gcaggaaatc agggattttg gaaagtggat ttatgttgtt
     6361 gctaaaaatc attgtctgat gcggttaaga aagcgagaga ttgtaattaa taatgaaaat
     6421 gccgggcatt ttattatgga atcagcagaa atcatgcatc ttaataatga agaaactctt
     6481 tataatgaag aaactctttc tgaaaataaa attaaaaaat tggaagcggg ggtagacgaa
     6541 ttgcctctgg aacaaaagat ctgtataaga ctgttttatt atgaagaaaa gagttattcg
     6601 caaatagcgg cggaaaccgg atatgacctg aagaaagtga agagttatgt tcagaatggt
     6661 aaaagaaatt tgaaaatttg gatggaaaat aaaagtgagc gaaaataaaa accatatagc
     6721 ccatctcagt ctttctaaaa tgaaggatta cctttctggc aagctcacgg aaaaggaatc
     6781 acatcaaatt gagatacata tgcaggaatg caatttatgc gcggatgcat tgatgggtat
     6841 ggagaggatg gatcctggaa ttattgggga agatgtgagt cacctccaaa gtcttgtaat
     6901 tgaaaaaacc agggcgagat caagaacttc atggaaggta agaattgcag ctgcagttgt
     6961 tatacttgct ataagctctt atttggtagt ttcttacctg cagttaggtg gcaagaatca
     7021 aatcgtagca tccgaagata gtggaactga aagtgatgct caggccagag aaggtgacgc
     7081 cttggccatg aaagattcca ttcaggaaat gaacaaacct gcggcagcag aagatattga
     7141 aattgccggc aggcagttgc ccgatgaaag agatcaaact tttatagaaa atgaaattga
     7201 tacaggtgca ggaacagatg aggtgaccat agacatgaaa gtagttcagg cagaaactga
     7261 ggttgatatg ccggagaagg gattagcgga agaagtaatt attactgagg cggaacctga
     7321 acttttggct gaccaaaagc ttgagggtgt taaagaagaa ttggtaaagg aagaagcgat
     7381 tttccggcgt aattccaaga gcgctgccca aagcatagct gatcagccgg ttgaagccgc
     7441 acctgaattg aatactctct ccggctttgc tgcaaaagaa gtcctactta taatccgggt
     7501 catccaggga aatgaaaatg ctgacaaaaa tgctgaacca gagtccggaa ccattgaata
     7561 tgtaaatcaa attctgaacg atctgcaata ccccgaaaaa gcatccattg ccgaagtcag
     7621 gggacgagtt actttgatat tccaggttgg agaggatggc tcactttctg aattcaaaat
     7681 cacacaaagc cttggttatg gctgcgatga agaagccatc cgcgtaatta aaaagggatc
     7741 ccggtggaac gcagccattc tggccggtaa acccgtgagc caacaaattg aggtcatggt
     7801 accttttccc aaatcaggta agtgattttc acgacttttt ctagaaatct gcggtgatct
     7861 gtgatatcag cgggattaag aaaaattttc tcccgcagat tacctggatt tgcgcggaaa
     7921 actccggaaa taaattctgc ggtgatcggc gattatcagc gggattagaa aaaattactc
     7981 ccgcggatta cgcggattta cgcagaaagc tccggaaata aattctgcaa tgatcagcga
     8041 gatcagcggg attaagaaaa ttttcttccg cggattacaa agttttacgc tgaaagtcct
     8101 cgaaccattt tttccccagt ggtgatctgc gatatcagcg ggattaaaaa aactttctcc
     8161 cgcagattac gcggatttgc gcagaaaact ccggaaataa attctgcgat gatctacatt
     8221 atcagcagga ttaagaaaga atttgctccc gcagatcact cggatttgcg tagaaaactc
     8281 cggaaataaa ttctgcggtg atctgcgtta tcagcgggat tagaaaaaat tactcccgca
     8341 gattacgcgg atttgcgctg aaaactccga aaataaattc agcggtgagc tgcgatatca
     8401 gcgggattaa gaaaaatttc tcccgcggat tacgcggatt tgcgcagaaa actccggaaa
     8461 taaattcagc ggtgagctgc gatatcaacg ggattctata aaatttgccc cttcggattt
     8521 gcgcggaaag ctctcgaacc atttttcccc ggtatgatct gcgatatcag cgggattaaa
     8581 aaaactttct cccgcggatt tgcggatttg cgcggaaagc tctcgaacca tttttccccg
     8641 gtatgatctg cgatatcagc ggg
//
LOCUS       DASWHX010000003         3593 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_2289, whole genome shotgun sequence.
ACCESSION   DASWHX010000003 DASWHX010000000
VERSION     DASWHX010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 3593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3593
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2289"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            354..1271
                     /locus_tag="VGA21_00040"
     CDS             354..1271
                     /locus_tag="VGA21_00040"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HEX9648918.1"
                     /translation="MKKLIIIRLSIILKFITLTATTVYAQVDPMFSQYDNNKIIVNPG
                     YAGINGDVNFTLLSRYQWVGLEGSPRTYTLSVHGPLRNRNVALGGSLVSDKIGVTSRN
                     SAIAYYAYQIKGDNNRIFSMGVQGGITLVTEKYSSLELSNDPTFSNDQSYIIPNFGFG
                     IYYSSNKFFGGISIPRLMENGFRENSGFTNTYVAQAGLNLGINRNINFIPYVMAKITG
                     EGTIQMDFNSQFEFGQHLMVGAGYRHGESILLKAGIIVSASFRLAYSYDSGLIGIAQN
                     GSHELMLNYRIPSRTSIKGNPRHIYSQMW"
     gene            1310..1966
                     /locus_tag="VGA21_00045"
     CDS             1310..1966
                     /locus_tag="VGA21_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013453322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX9648919.1"
                     /translation="MIPINIILVDDHKIIRDGLKTYLEGDNEFNVIGEASNGKEAMEI
                     IKETSPDIVVTDVMMPEMNGIELCKAIVDFNKNIKVIALTMMGDRQFIRQMLNNGVSG
                     YLLKSCTDDELKLAIKSVNGGKKYYSQEVTEAVMTDLMDGKSGKSKVAMDMPLSEREK
                     EVLHLVLKEYTNQEIADKLFISTRTVDAHKRNILDKTGSKNVAGLVFYALERKLFDDF
                     "
     gene            complement(1963..3390)
                     /locus_tag="VGA21_00050"
     CDS             complement(1963..3390)
                     /locus_tag="VGA21_00050"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9648920.1"
                     /translation="MARKISAIILEDSPADAELITFVINENFNSKLIILDDLDEFKNE
                     LSRNKYDLIISDFNLNQFGGLDALVAARAINKEIPFILVTGGINDKVAIDALKMGATD
                     YVLKNNLNKLPFTIERVMTEQEEYLRRKSVEIQREELLLQLTMKSDKLQALYNLSSIT
                     TQKSQLAVQELQQLGELIIHGFENPAEIGIKISCEDKEWCSTDVNIYSNNGIREIYNS
                     NELSLVIEVFDRNESNKTGSFSEGEVSFLKSVIEVVSNKIELQRSQDLLIRTIRETEE
                     KERKRIASEIHDNLQQMLVVVNQNLNILSKKISLDAENTSKPLEISKQYIMSAIKESR
                     RIAHNLAPHYSGDISTMIRELLNSFSDLPDISFQFESEVEEPYQNQNIDINLFRITQE
                     CINNIIKHANASNVTIKITGAFNLLSLSILDDGEGFDRKKIFEKDGHYGFDNMASRVN
                     SIGGSLNIDSRVGIGTHIKVNIPIR"
BASE COUNT         1147 a          648 c          665 g         1133 t
ORIGIN      
        1 gagggtggcg gcttcgccaa caccggttct aatgaaattc ctctaaataa tgattaacac
       61 ctttcccaaa tagtcaattt ctgacttctc gcccggtagg caattcccct aaattaataa
      121 aattgaaaat ttgccaagat agccgcaggg cttctgtcgt ttctttgtga agttgaaact
      181 aaaaaacaaa atgaaaaacg agattacaca aaatgaaatg aaactaaaca acaagattca
      241 taatagaata tttataatct tttggttgtt tatgtccggc gccttattgg ccgcttcctt
      301 actggtaaag tttggatttt aaggtaaatg tataacctat aaaaggagca agcatgaaaa
      361 agttaatcat catcaggttg tccatcatac ttaagttcat aacactgacg gcaaccaccg
      421 tatatgccca ggttgatccc atgtttagtc aatatgataa taataaaatc atagtgaatc
      481 ccggttatgc tggaattaat ggagatgtaa atttcacgct tctttcccgt taccagtggg
      541 ttggtttgga aggaagcccc agaacctaca ctttatccgt tcatgggcca ttaagaaaca
      601 gaaacgtagc attgggagga agtttggtca gtgacaagat aggtgtgacc agccggaatt
      661 ctgcgatcgc ttactatgct tatcaaatta aaggtgacaa taatagaatt ttctctatgg
      721 gcgtacaagg gggaattact ctggttacag aaaaatattc ctcacttgaa ttatccaatg
      781 atcccacatt ttcaaatgat cagagttaca taattcccaa ttttgggttt ggaatttatt
      841 actcatcaaa taaatttttt ggaggcattt caatacccag actaatggaa aatggtttta
      901 gagaaaactc cggttttact aacacgtatg ttgcacaagc aggcttgaac ctgggaatta
      961 accgaaatat taattttatt ccctatgtaa tggcaaaaat taccggtgaa ggcacaatcc
     1021 aaatggattt taactctcaa tttgagtttg ggcaacacct aatggttgga gccggctaca
     1081 gacatggcga atcgatcttg cttaaagctg gaattattgt ttccgcgagc ttcagattgg
     1141 cgtattctta cgatagcggg ttaattggaa ttgcacagaa tggttctcac gagttaatgt
     1201 taaattatag aattccttcc agaacctcaa taaagggaaa tccgagacac atctattccc
     1261 agatgtggta aaatcaaaaa aaataaagta aattaatgaa gaaattcaga tgataccaat
     1321 caacataata ctggtagatg accataaaat aatcagggat ggccttaaaa cctacctcga
     1381 aggagataat gaatttaacg ttatagggga agcctccaac ggaaaagagg caatggaaat
     1441 cattaaagaa acctcgccag atatagtagt tactgatgtt atgatgccgg aaatgaatgg
     1501 tattgaacta tgtaaagcta ttgttgattt caacaaaaat attaaagtta tagcccttac
     1561 tatgatggga gatcgtcaat ttataaggca aatgcttaat aacggagttt cgggctattt
     1621 gttaaaaagt tgtactgatg acgaacttaa acttgccatt aaatcggtaa atggagggaa
     1681 gaaatactac tcacaagaag tcacagaagc cgttatgacg gatttgatgg atgggaaaag
     1741 cggtaagtca aaagttgcta tggatatgcc attatctgag cgggaaaagg aagttttgca
     1801 cctggtcctt aaagaataca caaatcagga gattgccgat aaacttttca ttagtacccg
     1861 caccgtggat gctcacaaaa gaaatattct ggataagacg ggttcgaaaa atgttgcagg
     1921 attagtgttt tacgccctgg agcgcaagct ttttgatgac ttttagcgga taggtatatt
     1981 taccttgatg tgagttccaa tcccgacgcg actatcaata tttagtgacc ccccaattga
     2041 atttacgcga cttgccatat tatcaaatcc ataatgacca tccttttcga atattttttt
     2101 tcgatcgaaa ccttcgccat catcaagaat tgataaactt aataaattaa atgcaccggt
     2161 aatcttgatg gttacattgc tggcattggc atgcttgatt atgttattta tacactcctg
     2221 ggtaattcta aaaagattaa tgtcgatatt ctgattttgg tatggttctt ccacttcgct
     2281 ttcaaattga aatgaaatat caggcaggtc tgaaaatgaa tttagcaatt ccctgatcat
     2341 ggtactgatg tctcccgagt aatgtggagc caaattatga gcaatccgcc ggctttcctt
     2401 tattgcagac attatatatt gttttgatat ttcaagaggc ttggaggtat tttctgcgtc
     2461 taaggatatt ttcttggaaa gtatattcag attttgattc accactacta gcatttgttg
     2521 caaattgtca tgaatttctg aagcaattcg cttcctttct ttttcttccg tctcccggat
     2581 tgtccggatc aataaatcct gagacctttg caattcaatt ttgtttgaaa ccacttcaat
     2641 gacagattta agaaagctga cttctccttc agaaaaagaa ccggttttat ttgattcatt
     2701 tctgtcgaaa acctcgataa caagactcag ttcatttgaa ttatagattt ccctaattcc
     2761 attattggag tatatattca cgtcagtgga acaccactcc ttatcttcac atgaaatctt
     2821 tatgccaatt tcagccggat tctcaaaccc atgaataatc aattctccta attgctgaag
     2881 ttcttgaact gccaattggc ttttttgagt agtaatggat gataaattgt aaagtgcctg
     2941 aagtttatcg ctcttcatgg tcaattgaag taacaattct tcacgctgta tctcgacact
     3001 ctttcttcgc aggtattctt cctgttcagt cataactctt tcaatggtaa acggtagctt
     3061 attcagatta tttttgagga cataatcggt tgcgcccatt ttcaatgcat caatggcaac
     3121 tttgtcgttt atacctcccg taaccagaat aaaaggaatt tctttattga tcgccctcgc
     3181 agccacgagg gcatccagtc cgccaaattg attaaggtta aaatccgaaa ttattaaatc
     3241 atacttgtta cgtgataact catttttaaa ttcgtcaaga tcatccagaa ttattagttt
     3301 tgaattgaag ttttcattaa ttacaaaagt tattaattct gcatcagcag gggagtcttc
     3361 taaaattatt gctgaaattt ttcgtgccat ggatttctat cagtgagaaa atttgctcaa
     3421 taagttagta tttttaaagc aaaactcaat atgttgattt ccacatgtaa tggtgaagat
     3481 aagctctagg tattttacct aataaggata aatatgaaat ccaccgataa ttgcccatat
     3541 tcccgattgt acctttgacc taatccaaga tattttggag aaaacaaaaa aaa
//
LOCUS       DASWHX010000004        25283 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_2998, whole genome shotgun sequence.
ACCESSION   DASWHX010000004 DASWHX010000000
VERSION     DASWHX010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 25283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25283
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2998"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="VGA21_00055"
     CDS             <1..235
                     /locus_tag="VGA21_00055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9648921.1"
                     /translation="TVTVEGVGSLDIVLSGFVDNGVTITGRIENFPLGLDLNYALGSV
                     LPSSSPMFGLPSNPNNINFQPIVVDVVFTRTSL"
     gene            complement(230..1090)
                     /locus_tag="VGA21_00060"
     CDS             complement(230..1090)
                     /locus_tag="VGA21_00060"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9648922.1"
                     /translation="MNENLSGRYGEWAMVAGASEGIGAEFAKQLADHGFNLVLIARRE
                     SELAKKKAGLEKIVKVRTIAVDLSLKESISGIINQTLDLEIGLFVYNAVFPLIGHFID
                     QPIEKHHELIQVNISTPMTLVHHFGNIMVRRGKGAIILMSSMAGQQGSAMVAHYAASK
                     AYNTVLAEGLWYELKKRNVDVMAVIAGATSTPNYVNSKPEKTSMGTPAPLNPAAVVKE
                     ALARIGQNPSFITGRVNRIASLFMKMFFSRKKAIEIISKSTTEMYPHYLSKKKTRISR
                     AWFSKSFYGL"
     gene            complement(1096..1608)
                     /locus_tag="VGA21_00065"
     CDS             complement(1096..1608)
                     /locus_tag="VGA21_00065"
                     /inference="COORDINATES: protein motif:HMM:NF017090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="emopamil-binding family protein"
                     /protein_id="HEX9648923.1"
                     /translation="MRKLIPLKKRPLDIGILSFFWINILFITYLIDLEQLVIADVNNF
                     EYPLWPPPFIIDLVHWWGRNFDPALMAREPWWRATIWIDQLFFGPFYILAIYAFTKSK
                     EWIRIPSIVYASMLLTNVIIILWEEILGQHASNSIGIVLMANAAWVIMPLLIIFRFRN
                     NPHPFTEETD"
     gene            1669..2562
                     /locus_tag="VGA21_00070"
     CDS             1669..2562
                     /locus_tag="VGA21_00070"
                     /inference="COORDINATES: protein motif:HMM:NF018649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HEX9648924.1"
                     /translation="MAASDLKYSRTESFLFVIVAYAVALIIPWLMLPYLSEFHQLIKI
                     GILDIFATLIIFVFSYRLKNSSMYDPFWSVVPIPIVIYFMTLFPDGNFSRQILIVMLV
                     SFWGIRLTFNWVRGWHGLHHEDWRYQDLQKQHGKAYWTVSLTGIHLLPTILVFLGLIP
                     AYYAMLNPYPLNWIDIIASIVTFLAVIIEWVADEQLRSFRKLREFKSETIMEMGLWKY
                     SRHPNYFGEISFWIGLSLFGLNFNLNEYWWTFAGALSMILLFVFVSIPMMEKRSLESK
                     SGYKEHRKRISMLIPWIQKNN"
     gene            2610..3011
                     /gene="msrB"
                     /locus_tag="VGA21_00075"
     CDS             2610..3011
                     /gene="msrB"
                     /locus_tag="VGA21_00075"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015814207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEX9648925.1"
                     /translation="MNKIDKSEEDWKKELTEEQYYILREGGTEHAFAGKYNNNKDDGN
                     YYCAGCGNLLFDSKTKYDSGSGWPSFYQPIDKDKVIVQLDKTHRLIREEVLCANCGGH
                     LGHVFNDGPQPTGKRFCMNSAALDFKKRDPK"
     gene            3008..4228
                     /gene="lhgO"
                     /locus_tag="VGA21_00080"
     CDS             3008..4228
                     /gene="lhgO"
                     /locus_tag="VGA21_00080"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017729870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HEX9648926.1"
                     /translation="MIYDISVIGSGIVGLASALKILERKPGLKIQIIDKEAGPAFHQT
                     GHNSGVIHSGLYYKPGSLKTTNCIHGYSLLLKFCDEHEVPYELCGKIIVATAESQLKT
                     LETLYERGVQNGLQNLRKLSAEEVKEFEPHASGVRGLHVPQTGIIDFKKVAEKMLELI
                     QKIGCDVHYSEPVKKIVKQSGGNLIITPKNTYRSRLIVNCAGLHSDRIARMTGVAPTF
                     KIIPFRGEYYKIKKEKRHLVKNLIYPVPDPAFPFLGVHFTRMINGGVEAGPNAVLALA
                     REGYKKTNVNIRDIVESLTWPGFQKVAMKYWKTGLGEIHRSFSKKAFTDALRVLLPQI
                     ELEDLEPGGSGVRAQACDRDGGLIDDFMIIENDFAVNVCNAPSPAATSSLSIGETIAK
                     IAIQRFNSVSSYKF"
     gene            4354..5472
                     /locus_tag="VGA21_00085"
     CDS             4354..5472
                     /locus_tag="VGA21_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020529018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HEX9648927.1"
                     /translation="MEREDLKRYSRHLALPDFTEESQDKLKNSKVIVVGAGGLGSPVL
                     QYLTAAGVGTIGIIDYDVVEISNLQRQVIFTEADLGKPKAQVAAERLKLLNSKVKFNI
                     YLEKLTSDNALELLKPYDLIIDGTDNFPSRYLINDSSVILNKPFIYGSVYRFEGQVAV
                     FNYAYNDGKRGSNYRDLFPEPPPPDLVPNCEEGGVIGVMPGIIGTMQAAEAIKIITGI
                     GEVLSDKLLIIDIQTFQISKIKIQNKKDSTVINELIDYNEFCATKSKSMLFGPKVKEM
                     DSIALKELMDSGEEFQLIDVRESYEYDQDNLGGELIPLGQIMNESSKIRKDIKVIIHC
                     RSGSRSAAAIKHLEKQFGFDNLYNLKGGIINWHTKFGY"
     gene            5490..6107
                     /locus_tag="VGA21_00090"
     CDS             5490..6107
                     /locus_tag="VGA21_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008630468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HEX9648928.1"
                     /translation="MHIAVSGNIGSGKTTLTERLCRHYGWTPHFESVDNNPYLKDFYE
                     DMPRWAFHLQIYFLNTRFNQIKDIQANENTVVQDRTIYEDAFIFAAYLNEAGHISERD
                     YKSYLDIFKSMIDFVNPPDLLIYLRADIPKLVNQIQKRGRDFEYAMRLDYLRNLNTYY
                     ENWIKQYDLGKLLILDVNQLDFVTNVEDFSTVVEKVELELHGLFS"
     gene            complement(6108..7031)
                     /locus_tag="VGA21_00095"
     CDS             complement(6108..7031)
                     /locus_tag="VGA21_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020527593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5777 family beta-barrel protein"
                     /protein_id="HEX9648929.1"
                     /translation="MESKFLIKILLATALLLLVSFKVNAQDLMDLLEEETDEGKNYAT
                     AIFKGTRVVLGHSVKTRKTKELELFVMHRFGSMDNGMSDAFGLDDANIRIAFEYGITD
                     NLNIGIGRSSILKTYDGFIKYRFLRQSHPGSPVTMVVLAGLARNTNEVASDMDLDFRH
                     RMAYTYQFLIARKFTEGFSLQLTPSVTHKNLVTFREDDNTLFAIGLGGRLKLSPSVTF
                     NAEYFYQINPNKFQVNSLDNSYYPDAVSIGVDIETGGHVFQLHFTNATAMIDKAIIHE
                     TTGDVRDGKIRFGFNLSRTFQIVPKQGWSGN"
     gene            complement(7021..7560)
                     /locus_tag="VGA21_00100"
     CDS             complement(7021..7560)
                     /locus_tag="VGA21_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009579417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEX9648930.1"
                     /translation="MNRIFFLLAVLFLHDYSFGQKYITESSRISFYSEAPLEDIDAHN
                     NKTTSIFDSDKGEIAFSIPIKEFTFKRSLMQEHFNEKYMESEKYPKAAFNGAVTGFTK
                     KPGKQNATAEGELTIHGVTNKVTINGVMEFKDGKVFMNSSFIVKLEDYKIKIPSILFQ
                     NIAEEIEVKVELTYKEYGE"
     gene            complement(7565..7924)
                     /locus_tag="VGA21_00105"
     CDS             complement(7565..7924)
                     /locus_tag="VGA21_00105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9648931.1"
                     /translation="MNKSRFRVYLRICCLSVILSCSDDNEEDLFGSKNFFCDPDLVTF
                     SDIIKPIIDTNCTVSGCHVIGTGLPDFTQFSNIKTYAAEIRERTSTRDMPRGGRVLTS
                     LQIAQIGCWVESGALNN"
     gene            complement(8109..9866)
                     /locus_tag="VGA21_00110"
     CDS             complement(8109..9866)
                     /locus_tag="VGA21_00110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9648932.1"
                     /translation="MELTFNPYPGFWTIIVPGLVICSGFLIWKESFRKGRKLLRFISI
                     VLAVICLLAIAVRPSIRKSGSPRPLALLTMNYSQKDLDSLLIIRNNLVKIDLTAKRNQ
                     LEKILMENSVSEVFVMGDGLEIHELNLLSKIPSNFLPSDSIKGIVGLNYNTRIERGEM
                     LQLEIETKTDQDGFLVLEHSSGRIDSFATTRGVQKLNIKTPEKVSGNFILEIKLFDNK
                     KNLLDKGVVPYRVSDPEPIHVFFLQGYPTFETRFLKDWLQQKKHKVVVRSKVSRNDYV
                     LESFNIEKPLTNVINLNWIEKLDLIITDFEGWIDLSASEKDLIAKEIRDNGLGVLFLL
                     DEASRISKSELKMFISGNHFQQHNKMTAELILRESGVKFITPRIELKPGTNPFTFTIN
                     QDSKNEAISFGSFYGLGKVGFSLLPRTYTLALNGFMETYSDYWAEILDKLGKSWNKEY
                     VAINSSSISRVGLPVNLTISSTLENPQLKIDSIPIGLRQDLYFSDLWTCTFWPNKTGW
                     LPVQINNEIIDWIFIFPDEYWVNLELERKKNTNKRNLTFNVEGERKIVLENYPVSPLY
                     FYLIFTICIGLLWLEPRLN"
     gene            complement(9866..12034)
                     /locus_tag="VGA21_00115"
     CDS             complement(9866..12034)
                     /locus_tag="VGA21_00115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9648933.1"
                     /translation="MQESKAAVRALNSIQVKWNLLILFQILIISLPASFLFHQILNAF
                     SINAMLYSIPFLIVLSFIMAVVLKPGKIKSKQVGQLLDRQFEQLEDSSHLLFESDKFT
                     AVAKIQREKIIPLFDNLPTKVKIPVNFKVPLIVAGVLLLSSFLPGAFQNSKTNPPILT
                     QTSVVAPNEHSGEIESIRITNSQISIVPPSYTGLKPAIQEDFHLEIPSGSKVSWQLEF
                     NMPIETANLVWGLDNFNPMHIKGQSYIVETIISSSGFYSLAITSNNDTLKTEFYRVDV
                     IPDLPPKILTIQNELYQRLSFNAKIIIPVEAAISDDYGLSDAYLVTTVSKGAGEGVKF
                     REIKIPFAEKITGKKRVNLSATMDGSKLELEPGSELYYFIEAWDNKSPNPNRSRTETF
                     FLVIEDTSSTEFSGFGGLAVDLMPDYFRSQRQIIIETEKLIKDNPKLPIRNFKTISNE
                     LGFDQKVLRLKYSQFLGEEFLSGIELESDETPASDEALRILEESHEHDEELNPDAEED
                     PLEQVSHAHDTEEEVTFFSISVESKLRAALVEMWDAELYLRLYQPEKSLPYQYRALEL
                     IMEIKNHARIYVQRVGFDPPPLTEEKRLSGKQEEVDPRQTASINEVRAEFPNIRLVTT
                     VLDNAILSNRDLTENEKLLLVACGNEIAYLVETNPLQYLDILQEIKRISESDTQVGTQ
                     LKKLKSEFYEILISQSREPGSKAIPQDPLKANYFKSGRNF"
     gene            complement(12034..13287)
                     /locus_tag="VGA21_00120"
     CDS             complement(12034..13287)
                     /locus_tag="VGA21_00120"
                     /inference="COORDINATES: protein motif:HMM:TIGR02226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA domain-containing protein"
                     /protein_id="HEX9648934.1"
                     /translation="MNLIFLNPAAFWGLLALTFPLIIHLLNKSKGTTIWVGNISWIKK
                     VEKTRSRSIRLNEYTLLALRSLIFAALVFILANPVIQSNQTLVKRNLLLIDSEVAGMN
                     LINLKLDTTDYNAYDIRYLHNNKIVNSIDDSVFLRNNLFLALEELEGLNRYGKVVLFS
                     HGYLKNIAGAFPKFSFELTWYQIPSSPGEFPIVKKQFSKDSVLLIGQYLQGNNLISRT
                     EIIDAGSFKTGELGEVNSKFAIIVTEPGFDKEVEEWVRVLEAIARQPEFNFKFKGIYA
                     PESFSEDSLMNTYVIWIGPEPGKDLKSPGVINYNPLKTNNITGPISIIKSQVNFYETM
                     GRITPEAIYNEEFLRTVFELFFGGDVSPEKLQNSDYRQFTQEIEKQNMVAGPVSRQTD
                     FSYPFWLAVFSLIIGERILSKRRGQ"
     gene            complement(13284..14198)
                     /locus_tag="VGA21_00125"
     CDS             complement(13284..14198)
                     /locus_tag="VGA21_00125"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX9648935.1"
                     /translation="MAKLKESNEKISDLLRPEIISSIEGLELVARIVVEEFFHGNNRS
                     HRLGMGQEFSQYRSYEPGDDLRLLDWKMYARSERYYIRQSEIDTHITIRFIIDASKSM
                     EHESGGLRKIDLARIIVASLAYLARNQGDSFGLFTINNENISRLSPTVHPQQFTRLLQ
                     MLIQLKTAGAWPIRPARLEDIYYPGRKEMVIFLSDMYEKENELMDFITSLKTQRNEVL
                     VLNMVAQNEIEFAYEGFHTFKDLETGEEVLTDADKAREFFVEKKRIAENHFQTTFLAK
                     EIDYHQFNSGDDLPTLLSGFLQIRKRLV"
     gene            complement(14185..15186)
                     /locus_tag="VGA21_00130"
     CDS             complement(14185..15186)
                     /locus_tag="VGA21_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009577641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX9648936.1"
                     /translation="MEETKERVLALINKLPQLKKEIGKVIIGQEETIDHLLITFMANG
                     HALLEGVPGLAKTLMIRTLAKCLDLSFRRIQFTPDLMPSDILGTEILEEDHATGKKFF
                     KFNKGPIFANIILADEINRTPPKTQSALLEAMQEKEVTYGGKTYTLDKPFFILATQNP
                     IEQSGTFPLPEAQLDRFLLYIRIGYPGESEEKTVLEQTTGTVTAEVSRVINGNDIIEI
                     QNLVRQVPISDDLVEYVNKLVRATRPDNTISEYVKEWVRWGAGPRAGQAIILTAKARA
                     LLNARFAVTVDDIRHVAYPVLRHRIILNFKAESAQIQPDMVTEKLLDTIRLPMNGKA"
     gene            complement(15189..15743)
                     /locus_tag="VGA21_00135"
     CDS             complement(15189..15743)
                     /locus_tag="VGA21_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009280022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HEX9648937.1"
                     /translation="MPSNILNSLIEYTTLEVDTEEKVIPLSSDEIFYSPFCYLSGHKL
                     VQFTDKESKNFKRYVENGGFIFADDCNHDIDGLFAKSFEEQMLRIFGKGILRKLPDNH
                     QIYNCFFRFEKGPPTTSQELNGWGDDLVHDYLKAVEINTRIGVLYSNKDYGCEWDYDF
                     RNKKWYKNDNTKFGVNLVIYALTS"
     gene            complement(15950..17266)
                     /locus_tag="VGA21_00140"
     CDS             complement(15950..17266)
                     /locus_tag="VGA21_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007652633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HEX9648938.1"
                     /translation="MAILTKDEAQQILQKALSFSKADECEVNLGSTDEGNIRYARNTV
                     STSGYQSNIQLVAGSSFGKKTGIATINELDDASLEKVIRRSEELAQLSPENPEFVPIL
                     GPQEFAESKSFVESTAKISAEYRTKVAESSIGPARTSDITAAGFFNDSVSMNSMMNSK
                     GLFAYNRRTNIDFTVTMRSNDGTGSGWVTRDYNDVNKFNSEEASKIAIDKALLSKEAQ
                     AIEPGKYTVILEPAAAHDLLQNMLFNFNARNADEGRSFLAKEGGGTKKGEKIVDERVT
                     IYSDPLHPEVPAATWNGNGEPLNKTMWLENGIVKNLFYSRYWAEQKGVEPVPGPSNGI
                     MVGGDASLEDLIKDTTRGILVTRFWYIRTVDPQTLLYTGLTRDGTFFIENGKIKHPIK
                     NFRFNESPVIMLNNLEALGKQQRVNGNLIPAMKVRDFTFTSLSDAV"
     gene            complement(17296..18936)
                     /locus_tag="VGA21_00145"
     CDS             complement(17296..18936)
                     /locus_tag="VGA21_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015030632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HEX9648939.1"
                     /translation="MKRREFIQWTGLGVGAAMLPIPILGTPVSEEVLLTPFLDVTAKK
                     KLADIALNSAKALGATYVDTRIGRYLNQFVFTREDKVQNVVSTESFGIGVRVIANGTW
                     GFASTHDVSPESIKKAAELAVSIAKANSRIQKEPVILAPAQSHGEVAWKTPIEKNCFE
                     VPVSEKVELLLSANASAMENGASFVNSGLFMVNEQKYFASSDGSYIDQDVHRIWPTFN
                     VTAVDRAAGKFKTRQSLSAPMGMGYEYMSGDASGKVEGPGGLIHYSKYYDIIEDATAA
                     AKQAKEMLTAKSVEAGKFDLVLDPNHLGLTIHESVGHPLELDRVLGYEANYAGTSFAT
                     MEKWESKSFNYGSKLVNFIADKTQVGSLGAVGYDDEGVKCKEWDLVKEGILVNYQAIR
                     DQVHILGEKSSHGCCYAQSWNDVQFQRMPNVSLKSGSEKYSSMDMVKDVEKGIYIIGR
                     GSYSIDQQRYNFQFGGTLFYEIENGEIKGMLNDVAYQSNTQEFWNSCVKICDESDYRF
                     FGSFFDGKGQPSQVSAVSHGCSTSRFNGVNVINTGRTI"
     gene            19092..20165
                     /gene="dinB"
                     /locus_tag="VGA21_00150"
     CDS             19092..20165
                     /gene="dinB"
                     /locus_tag="VGA21_00150"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020533585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEX9648940.1"
                     /translation="MFKKIIHIDMDAFYASVEQRDNPNLANLPVAVGWDSSRGVIATA
                     SYEARKFGVRSAMLVSAGKKLCPELIIVEPRMDAYKNASVQIRSIFDEYTDLVEPLSL
                     DEAYLDVTNNKLNLRSATIIAREIKRKIKNQTHLTASAGISINKFLAKIASDMNKPDG
                     LTLIHPNKALEFLKQLKIEKFHGIGKVTARRMRNFGISKGIDIIHSGEEFMLKNFGKN
                     GKFFFQIAQGIDNREVSPARERKSVSVENTFAEDLTITEDIHYELRKLAYVLAKRIHR
                     NQFISKTLTLKIKFSDFKIINRSKTGDNLIADEQQIRELGESMLDHIQIEKGVRLLGL
                     GVTKPGGNKEDSANSPSQLTLDF"
     gene            complement(20162..20722)
                     /locus_tag="VGA21_00155"
     CDS             complement(20162..20722)
                     /locus_tag="VGA21_00155"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009197104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEX9648941.1"
                     /translation="MGEATISKAIIVPSRMVFFMWLIYTGEKYVNFDLGIFGIYPRTW
                     FGLIGVITGPLIHGNLMHLASNTFPLLFLGTTLYFFYSRIAGKVFIQCYLGTGVLVWI
                     FGRESFHIGASGVIYALAGFLVFFGLFRKDLKSIFISIVILSVYWTLVYGIFPGQYGI
                     SWESHLFGALVGGATAFVYSKFSKVS"
     gene            complement(20724..21191)
                     /locus_tag="VGA21_00160"
     CDS             complement(20724..21191)
                     /locus_tag="VGA21_00160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9648942.1"
                     /translation="MIPEFEKLNNDEVDLMFKAPLLTAILVAGADDTVDRSELKEAVN
                     IITLRPQKSRKELVDYYTLVAEDVEDKLKVLLHEFPSNTIKRTDMITDELKKLNSIFP
                     KLDKNFSIRFYESLIEIALRIAESSGGVFGFMSVGYEESRVIDLKMIKDPSQL"
     gene            21315..22664
                     /locus_tag="VGA21_00165"
     CDS             21315..22664
                     /locus_tag="VGA21_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009580796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX9648943.1"
                     /translation="MEEPLPFKIFVIEDDPTYRKFLEYVLSLNPDFEVSTFENGKSFI
                     ESLDKNPSLVTLDYSLPDIKGEDVLSYIKKYNPDIPVIVISAQEKIGTAVSLLKLGAF
                     DYIVKDDETKDKLLNTINNARRNISLITEIVSLRREISEKYEFSKSIIGNSKAIKDVF
                     KLLEKAVSTNITVSITGETGTGKELVAKAIHYNSARKNKKFVAVNIAAIPKELIESEL
                     FGHEKGAFTGAASRRLGRFEDANRGTIFLDEIGEMDINLQAKLLRVLQEKEISRIGGN
                     EVIKLDVRVIVATHKDLPEEVSKGNFREDLFYRLLGLPVHLPPLRERDNDIILIAKSL
                     LRNFCKENRIPEIQISVAAQEKLMQYSFPGNVRELKSIIELAAVMCNGESINSDDLKF
                     GNLARLEKLLTEELTLRELNIKVIKYYLEKYDSNVKLVADKLGIGKSSIYRYLNNEQ"
     gene            22671..24575
                     /locus_tag="VGA21_00170"
     CDS             22671..24575
                     /locus_tag="VGA21_00170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9648944.1"
                     /translation="MKADSISIRNYINKGSFYRAVVEDGADIIFIVDYEGIIYYHNPS
                     VKNFLGYSDGELIDKNFIEQNHPEYRSRLTKTFKEISKEKYADNIEFYFRKKNGEYTY
                     LEFNSINLHHKDQIDALLLDCRDISQRKKDAEELIKAQKAKELFLANMSHEIRTPISG
                     IAGMVTLLEQFDRNPEEMKYLNAIKHSAEHLKIIINDILDFAAIEAGKLTFENIGFRP
                     YEQAGNVVNSFEYNLKDKGLEIDFSFDGEKDLIVKGDPVRLNQVLVNLINNAIKFTPQ
                     GGVNLSVKISKQGEKYLATYCVKDTGIGIPHNKRMNLFESFSQADTSINRRFGGTGLG
                     LAISKHIVNIQGGRIWFESEVDQGSTFCFEIPFEPGNVSDLVTEKKEKKSDETSLDGL
                     RVLLAEDNEINKLYTTKVLSGLNCKVETVENGKDCVDLLEKNNFDLVLMDLQMPIMDG
                     FEATKIIREKLPNPKKEITIIALTANAIKGDNEKCIAAGMNDYLSKPFTPENLKKKIL
                     RWVKRPGHSEITKASSDSVIDLTYLRTVCNNDESFLKEMITTFRNSTPELLDQMESAL
                     SQDDIITVSKIAHKIKPSISFMGIQSALQGVSEIENTKAAPLPSGYNQMVLDFLKEIR
                     KALIELDTIR"
     gene            complement(24578..>25283)
                     /locus_tag="VGA21_00175"
     CDS             complement(24578..>25283)
                     /locus_tag="VGA21_00175"
                     /inference="COORDINATES: protein motif:HMM:NF039201.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEX9648945.1"
                     /translation="ATDIDINKRALIDPQFDIAVTELSAVESDISIRTTITSRNDLVG
                     QQLVVHNAIVEEEILNGSGTVYYNVLRKLLPDAAGTLYTQDWNTGGSATFDLTWPIDV
                     KISDATKLAVITFIQNRATREIYQTIITQLGDKMPPLIVGLEEDIKSRLNQANIYPNP
                     VDQYLNVEIPNPGPYNYTYKIVDQRGVTINQGDVELKGNLFEMEVSEINNGIYFLVIG
                     VENRPFTYKKIAVMHR"
BASE COUNT         7741 a         5031 c         4830 g         7681 t
ORIGIN      
        1 gactgttacg gttgaaggtg ttggatcatt ggacattgtc ttaagcggct ttgtggataa
       61 cggtgttact attacaggaa gaatcgaaaa cttcccctta ggattggatc ttaattatgc
      121 attaggttcc gttttaccaa gcagtagccc gatgtttggg ttaccttcca atccaaataa
      181 tatcaatttt caacctattg ttgtagatgt agtatttacg agaacaagct tataatccgt
      241 aaaaagattt tgaaaaccag gcccggctaa tccgggtctt ttttttgctc agatagtggg
      301 gatacatttc agtggtagat ttacttataa tctcaattgc ttttttcctt gaaaaaaaca
      361 ttttcataaa caacgaagcg atccggttta ctcttccggt tataaaactt gggttctgcc
      421 caatccttgc cagggcctct ttcacaacag cggctggatt taaaggtgcc ggagttccca
      481 tgctggtctt ctcaggtttg ctgttaacat agttgggtgt tgaagtagca ccggcgatca
      541 cagccatcac atccacattc cgctttttaa gttcatacca cagaccttcc gctaatacgg
      601 tattataggc ttttgatgcc gcataatggg ctaccatagc tgatccctgc tgacccgcca
      661 tgcttgacat aagaataatt gctccttttc ctcttctcac cataatattt ccaaagtgat
      721 gcacaagagt catcggagtt gaaatattta cctgaataag ctcatggtgt ttttcaatcg
      781 gttgatcaat aaaatgacct ataagtggaa atacggcatt gtaaacaaat aaaccaattt
      841 caagatccag ggtttgattg atgatgccgg aaatactttc ctttagtgac aaatcaaccg
      901 ctattgtccg caccttaact attttttcca gccccgcttt ctttttagcc aattctgatt
      961 cgcgccttgc aataagaact aaattaaacc catggtcagc aagctgtttt gcaaactctg
     1021 ctccaattcc ttccgaggca cctgcaacca tcgcccattc cccatatcta cctgataaat
     1081 tctcattcat agtgattaat ccgtttcttc agtaaaagga tgtgggttat ttctgaatct
     1141 gaaaataatt aatagtggca taattaccca tgcagcatta gccatcaaaa caattcctat
     1201 gctgtttgaa gcatgttggc ccaaaatttc ctcccaaaga ataatgatga cgttggttaa
     1261 taacattgaa gcgtaaacaa tgctggggat ccggatccat tccttgcttt tggtaaaagc
     1321 ataaattgcc aggatataga atggaccaaa gaataattga tcgatccaga ttgttgcccg
     1381 ccaccatggt tcgcgagcca ttaaggccgg atcaaaattc cttccccacc aatgcaccaa
     1441 atcaataata aacggcggag gccaaagcgg gtattcaaaa ttgttcacat ctgcaattac
     1501 cagctgctcc aaatcaatta agtaggtaat aaaaagaata ttgatccaga aaaaggaaag
     1561 tataccaata tccagggggc gtttcttcaa tggaatgagc tttctcattt ttaaactact
     1621 tttgaagagt aaatataaat agtaaaacct cagtatttta aaaatttcat ggccgcttca
     1681 gacttaaagt atagcaggac agagtcattt ttgtttgtga ttgtcgctta tgcggtggct
     1741 ttgataattc catggttaat gcttccttat ctgagtgaat ttcaccagct aatcaaaatt
     1801 ggaattcttg atatttttgc aaccctaatc attttcgttt ttagctaccg tctcaaaaat
     1861 tccagcatgt atgatccatt ctggagtgta gttcctatac cgatagtgat atattttatg
     1921 accctgtttc cggatggaaa tttttcacgg caaattctta ttgtcatgtt agtgtcgttt
     1981 tggggaataa gactaacctt caattgggtg cggggttggc acggactaca tcacgaagac
     2041 tggagatatc aggacttgca aaaacaacat ggaaaggcat actggactgt cagccttacc
     2101 ggaattcacc ttttacccac aatacttgta ttcctcggat taattccagc gtattatgcc
     2161 atgttgaatc cctacccgtt aaattggatt gatatcattg cctcaatagt aacatttcta
     2221 gcagtaataa ttgaatgggt tgcagacgaa cagctgcgaa gtttcaggaa actccgggag
     2281 ttcaaatccg aaaccatcat ggaaatggga ttatggaaat atagccggca tcccaattat
     2341 tttggagaaa tctccttctg gattgggctg tctctatttg ggttaaattt caatctgaat
     2401 gagtattggt ggacgttcgc cggtgcgtta agtatgattc tgctatttgt ctttgtcagc
     2461 attcccatga tggaaaaacg ttcccttgaa agcaaatcag gttacaagga acacaggaaa
     2521 agaatttcaa tgctcattcc atggattcaa aaaaataatt aaattaagaa ttaagccata
     2581 ttacttattt aacttaaatt cagtattata tgaataagat tgataaaagc gaggaagatt
     2641 ggaagaagga attgacggag gagcaatatt atattttaag agaaggagga accgaacacg
     2701 catttgccgg aaagtacaat aacaataaag atgacggtaa ttattactgt gccggatgtg
     2761 gtaatcttct ttttgattcg aaaacaaaat acgacagtgg gtcgggctgg ccaagctttt
     2821 accaacctat tgataaggac aaagtaattg ttcaattgga caagactcac cgcttgatcc
     2881 gcgaagaagt tctttgtgct aattgcggag gtcatttggg tcatgttttt aatgatggac
     2941 cacaaccaac gggcaagcga ttttgtatga actcggcagc ccttgacttc aagaagcgag
     3001 atccgaaatg atctacgata ttagcgtaat tggaagcggc attgttggcc ttgcatcagc
     3061 cctaaaaatt ctggagcgta aacctggcct gaaaattcaa attattgaca aagaagccgg
     3121 gcccgcattt catcagaccg gacataattc aggggtaatt cactcaggtc tttattataa
     3181 acctggaagt ctcaaaacca ccaattgcat tcatggatac tcattgctgc ttaagttttg
     3241 cgatgaacat gaagttccct atgagctttg cgggaagatc attgttgcga cagcggagtc
     3301 ccaactaaaa accctggaaa ccttgtatga aagaggtgtc caaaacggac ttcagaatct
     3361 cagaaaactg tcggcggaag aagtcaaaga gtttgaacct catgcatcgg gagtcagagg
     3421 attacacgta cctcagacgg gaatcataga cttcaaaaaa gtagctgaga aaatgcttga
     3481 gctgattcaa aaaattggct gtgatgttca ttattcggaa ccggtgaaaa aaattgtaaa
     3541 acaatcgggt ggaaatttga ttattacacc gaagaacact tatcgaagca gattaatcgt
     3601 gaactgcgca ggtctccatt cagatcgtat tgccagaatg accggtgtag caccaacctt
     3661 taaaattatt cccttccgtg gtgagtatta taagatcaaa aaagagaaac gtcacctggt
     3721 gaagaatctt atctacccgg ttcccgaccc tgcttttccc ttcctgggag ttcattttac
     3781 aaggatgata aacggaggag tggaagcagg acctaatgcg gtgctggcgc ttgccaggga
     3841 aggctataaa aaaacaaatg ttaacattag ggatattgta gaaagcctta cctggccggg
     3901 atttcaaaaa gttgcaatga aatattggaa gacaggtctt ggtgaaatac atcgatcatt
     3961 ttccaaaaaa gcatttacgg atgctctcag agtattatta ccccaaattg agttagaaga
     4021 tctggaacct ggcggaagcg gcgtccgggc tcaggcctgt gaccgggatg gtggcttgat
     4081 agacgatttt atgattattg aaaatgattt cgcggttaat gtctgcaatg caccttcacc
     4141 ggctgcaact tcttcccttt ccattggaga aacaattgct aaaattgcaa ttcaacgttt
     4201 taattccgtc agcagttata agttctagtt taatttaacc ttttctaaaa cctgcatccc
     4261 ttaagccttg ctaatcatgc agggactgaa gaccttattg gcgaaagaaa gatgacaaac
     4321 aatcatgttt aagtatattt gaatctggtt aagatggaaa gagaagatct aaaaagatac
     4381 agtcgtcatt tggcccttcc ggattttacg gaggagtcac aggataaatt gaaaaattca
     4441 aaagttatag ttgtcggtgc cggcgggctt ggatcaccag tattgcaata tcttactgcc
     4501 gccggagttg gaacgattgg cataatagat tatgatgtgg tggaaatcag taacctgcaa
     4561 cgacaggtaa tttttacaga agccgattta ggaaaaccaa aggcgcaggt ggctgccgaa
     4621 agacttaaac tcctgaattc aaaggtaaaa tttaacattt acctggagaa acttaccagc
     4681 gataacgctt tggaattatt aaagccgtat gatcttataa ttgatggaac ggacaatttt
     4741 ccaagccgat acctgattaa tgattcgtct gttattctta acaaaccttt tatttatggt
     4801 tctgtataca ggtttgaagg gcaagttgcg gtattcaatt acgcgtataa tgacggaaag
     4861 cggggatcga attaccggga tttatttccg gaacccccac ctcctgattt agtcccgaat
     4921 tgtgaagaag ggggtgttat tggagttatg cccggtatta ttggaactat gcaggctgca
     4981 gaggcgataa aaattattac aggaataggt gaagtattat ccgacaaact tttgataatt
     5041 gatattcaaa cattccagat ttcaaaaatc aaaattcaaa ataaaaagga tagtacagtt
     5101 attaatgaat tgattgatta taatgaattt tgtgcaacta aatcaaagag tatgttattc
     5161 ggaccgaaag taaaggaaat ggattccatc gcgctgaagg agctgatgga ttccggagag
     5221 gaatttcaat taatagacgt aagagaatcg tatgaatacg atcaggataa tcttgggggt
     5281 gaattgatcc ctctgggaca aataatgaat gagtcatcga agatcagaaa agacattaaa
     5341 gttattatac attgcaggag cggctccaga agtgcggcgg caatcaagca tctggagaag
     5401 caattcggtt ttgataatct ctacaatctt aaaggcggta taattaactg gcacacaaaa
     5461 ttcggctatt aataaaatta tttcattaaa tgcatattgc agtctccggt aatatcggct
     5521 cgggtaaaac tactctgacg gagagattat gccgtcatta cggctggact ccgcactttg
     5581 aatccgtaga taacaatcct tatttaaaag atttctatga agacatgcca aggtgggctt
     5641 ttcacttgca gatatatttt ttgaataccc gatttaatca gattaaagat atccaggcta
     5701 atgaaaatac cgtggttcag gacaggacca tctatgaaga tgcatttatc tttgccgctt
     5761 atctaaacga agccggtcat atatcagaaa gggattacaa gagttacctg gatattttca
     5821 aatctatgat tgattttgta aatccacccg atcttttgat ttatctgaga gcagatatcc
     5881 caaaacttgt caatcaaatt caaaaacgtg gacgcgattt tgaatacgcc atgaggcttg
     5941 actacttaag aaatctcaat acatattacg aaaactggat taagcaatat gacttgggca
     6001 aattgttgat acttgatgta aatcaattgg attttgtaac taatgttgaa gatttctcaa
     6061 ccgtagttga gaaagtggaa ctggaacttc atggattgtt cagctgatta attcccgctc
     6121 catccttgtt taggcacaat ttgaaatgtc ctggataaat taaagccaaa cctgattttc
     6181 ccatcccgca catcaccggt tgtttcgtgg atgatagctt tgtcaatcat cgcagtggca
     6241 ttggtaaaat gcagctgaaa aacatgaccg ccggtttcaa tatcaacacc tattgataca
     6301 gcatcggggt aataggagtt gtcaagcgaa ttcacctgaa atttattagg attaatctgg
     6361 taaaaatact cagcattaaa tgtaacactg ggtgataact tcaatcttcc acccaaacca
     6421 attgcaaaca gcgtgttgtc gtcttctctg aatgttacaa gatttttatg agtaactgaa
     6481 ggtgtaagtt gaagcgaaaa cccttcggtg aattttcttg caataagaaa ctgataagtg
     6541 taagccattc tgtgcctgaa gtcaagatcc atatctgaag caacctcatt ggtatttctt
     6601 gccaagccgg ccagtaccac catggttacc ggtgagcccg ggtgagattg ccgcaagaat
     6661 ctatatttga tgaagccatc ataggtttta aggatagaac ttcttccaat cccaatattt
     6721 aaattatcag taataccgta ctcaaatgca attctaatat tcgcatcatc caaaccaaat
     6781 gcatctgaca taccattatc cattgatccg aaacggtgca tcacaaataa ttcaagctcc
     6841 tttgtttttc tggttttgac ggaatgacca agaaccacgc gggttccttt gaatattgca
     6901 gttgcataat tcttgccttc atcggtctct tcttccaaaa gatccattaa atcctgggcg
     6961 tttactttaa atgaaactaa taagaggagt gcggtggcaa gtaaaatttt aatcaggaat
     7021 ttactctcca tattccttat atgttaattc taccttaacc tcaatctctt cggcaatgtt
     7081 ttgaaacaaa atggaaggaa tttttatttt ataatcttca agtttaacaa taaaggaaga
     7141 gttcataaag actttgccgt ctttaaattc catcactccg tttattgtta ctttattagt
     7201 tacaccatgg atagttaatt ctccttcagc ggttgcattt tgtttgccgg gctttttagt
     7261 gaatcctgta acagcgccat tgaaggccgc cttaggatat ttttcactct ccatatattt
     7321 ttcgttgaaa tgctcctgca tcaatgatcg tttgaaagtg aattctttga taggaataga
     7381 aaatgcgatt tctcccttat cactatcaaa gatgctggtg gttttattat tgtgcgcgtc
     7441 aatatcttcc agaggtgctt ctgaataaaa acttattctt gaagattcgg taatatattt
     7501 ttgtccaaat gagtaatcat gaagaaataa tacagccagt aaaaagaaaa tccggttcat
     7561 cgttttaatt gtttagcgct ccactttcta cccagcagcc aatctgagct atttgtaaac
     7621 tagtaagaac tctccctcct ctgggcatat ctctcgtaga agtacgctcg cgtatttcag
     7681 ccgcatatgt tttaatattt gaaaactggg taaagtcggg cagtcctgtt ccgataacat
     7741 gacaaccact gacagtacaa ttggtatcaa tgatcggctt aatgatgtcg gaaaatgtta
     7801 ctaaatccgg gtcacaaaaa aaatttttcg agccaaaaag atcttcctca ttatcgtcag
     7861 aacatgacaa aatcacagat aaacagcaga ttctcaggta tactctaaat ctggatttat
     7921 tcattattta taattaattt agaattaaga gtttagacac tcaactaagc aagtaatggg
     7981 ccgaaaatac gaaattagcc cctatttccc tggaaaggtt gtatttgatc ctataaccca
     8041 cataaaataa agcagtgcta tcataaaaat tgcgaaaatg aatatgaaaa ttaattttga
     8101 acgcttcatt aattaagtct gggctccagc cacaataaac cgatgcaaat tgtgaaaata
     8161 agatagaaat ataatggcga aacggggtaa ttttccaata cgatttttct ttctccttca
     8221 acattgaatg tcaaatttcg tttattcgta ttcttcttcc tttcaagctc caggttcacc
     8281 cagtattcat ccgggaaaat gaaaatccag tcaattattt cattattaat ttgaactgga
     8341 agccaaccgg ttttatttgg ccagaatgta caggtccata aatcactaaa ataaagatct
     8401 tgcctaagcc caattgggat actgtctatt tttagctgtg gattttcaag ggttgagctt
     8461 atagtgagat ttacgggtag ccctaccctg cttattgacg atgaatttat tgcgacgtat
     8521 tccttattcc agctcttgcc tagtttatca agaatttcag cccaataatc tgaataggtc
     8581 tccatgaaac cgtttaaagc aagagtatag gttcgaggca acagggaaaa accaaccttt
     8641 cccaaaccat aaaaagaacc aaaacttata gcttcattct ttgagtcttg atttatcgtg
     8701 aatgtaaatg ggtttgttcc tggtttaagc tcaatcctgg gagtaatgaa tttgactcca
     8761 ctctcacgaa gtatcaattc tgctgtcatt ttgttgtgtt gctgaaagtg attccctgag
     8821 ataaacattt tcaattctga ttttgaaatc cgggaggcct catccagcag aaataagact
     8881 cccaaaccat tatcgcgaat ttccttggcg ataagatctt tctccgaagc gctaagatcg
     8941 atccatcctt caaaatcagt gattatcaaa tccagttttt caatccaatt taagttaatg
     9001 acatttgtga ggggtttttc gatgttaaag ctttctaaaa catagtcgtt cctggaaacc
     9061 tttgatcgta ctactacttt atgctttttt tgttgcagcc aatctttcag aaacctggtt
     9121 tcaaaggttg ggtatccctg aagaaaaaat acatggatag gttccggatc tgaaactcta
     9181 taagggacta ctcctttatc aagtaaattc tttttattgt caaaaagttt tatttctaaa
     9241 ataaaattac cacttacttt ttccggcgtc ttaatattaa gtttctgcac accgcgggtg
     9301 gtagcaaagg aatcaattct tccagacgaa tgttccagca ccaggaatcc atcctggtcg
     9361 gtttttgttt caatttccaa ctgtaacatc tctccgcgtt caatccttgt attgtaattt
     9421 aaacctacaa ttccttttat tgaatctgaa ggcaagaagt tcgaggggat ttttgacaga
     9481 agattcagct catgaatttc aagtccgtca cccattacaa agacttcact cacagaattc
     9541 tccattagaa ttttttccaa ctgattcctt tttgcggtca gatcaatttt tacaagattg
     9601 tttcttataa taagcaaact atccaaatct ttttgtgaat aattcattgt gagtaacgcc
     9661 agtggccttg gcgatccaga ttttcttatg gacggcctaa cggcaattgc aagcaaacaa
     9721 atcaccgcca gaactatcga aataaatctt aataattttc gtcctttccg aaatgattct
     9781 ttccatatta agaatccgga acaaatgaca aggcccggaa caattatggt ccagaaacct
     9841 ggatatggat tgaaagttaa ctccatcaaa aatttcttcc cgatttaaaa taattggctt
     9901 tcaggggatc ctgtggtata gccttagatc ctggctccct ggattgactt ataagtattt
     9961 cataaaattc tgattttagt tttttcagtt gtgtccccac ctgagtatcg ctttcggaaa
    10021 ttctttttat ctcctgtaaa atatcaaggt attgcagggg attagtttca accaggtacg
    10081 cgatttcatt tccgcaagcc actagtagta atttctcatt ttccgtcaaa tcccggtttg
    10141 aaagaatagc attatccagt acagtggtca ctagccttat gttaggaaac tctgccctga
    10201 cttcattgat agaagctgtt tgtctcggat ctacttcttc ctgcttgccg gaaagccttt
    10261 tttcctctgt caacggaggg gggtcaaatc caacacgctg aacatatatt cttgcatgat
    10321 tctttatttc cataatcaat tccaaagccc tatactggta tggcaatgat ttctccggtt
    10381 gataaagacg aagatataat tcagcatccc acatctcaac cagcgctgct ctcaatttcg
    10441 attctacaga gattgaaaaa aaagtaactt cttcttcagt atcgtgagcg tgactgactt
    10501 gctccaaagg atcctcttca gcatccggat tcaattcttc atcatgttca tggctctcct
    10561 ctaatatccg cagcgcttca tcggaagcag gagtttcatc actttccaat tctatgcctg
    10621 ataaaaattc ctcacccaaa aactgactat actttaatcg aagcactttt tgatcaaagc
    10681 cgagttcatt acttatagtc ttaaaattcc taataggtaa tttagggtta tcttttatca
    10741 gcttttctgt ctctataata atctgtcttt gacttcgaaa gtaatcaggc attagatcta
    10801 cggcaaggcc accaaagccg gagaattcag ttgagctggt atcttctatc acaagaaaga
    10861 aagtttctgt tcttgaccgg ttaggattag gcgatttatt atcccaagcc tcaataaaat
    10921 aataaagctc tgaacccggc tccaattcca atttacttcc atccattgtt gctgaaagat
    10981 ttactctttt cttccctgta attttctcag caaaaggtat ttttatttct ctgaatttca
    11041 cgccttcacc agctccttta cttacagtag tgacaaggta agcatcagat aacccgtagt
    11101 catctgatat agcagcttca accggtataa taattttggc attaaaactt aagcgctgat
    11161 acaattcatt ttgaatagtt aatattttgg gcgggagatc aggaataaca tcaacccggt
    11221 aaaattcagt ttttagtgtg tcgttattac ttgtaatggc aagagaatag aatccactcg
    11281 aagaaataat ggtttcaaca atataagact gtcccttgat gtgcattgga ttaaaattat
    11341 ccaaacccca aaccaggttt gcggtctcga tgggcatatt aaattccagt tgccaggaaa
    11401 ctttgctgcc tgaaggaatc tccaggtgaa aatcttcctg aatagccggt ttcaatccag
    11461 tataggatgg aggcacaatg cttatctggc tatttgttat tcttattgat tcaatctctc
    11521 cggaatgctc atttggggct acaaccgagg tttgagtaag aatcggtgga ttggttttcg
    11581 agttctgaaa agcgccaggc aagaatgatg aaagcaacag gacccctgca actatcagcg
    11641 gaactttaaa gtttaccgga attttcactt tagttggtaa gttatcgaag agaggaataa
    11701 tcttctcgcg ttgaatttta gctaccgcag taaacttatc tgactcaaag agtagatgac
    11761 tgctgtcctc caactgttca aactgccggt ctaataattg gccaacctgt ttacttttaa
    11821 tttttccagg ttttagaaca actgccataa taaaagacag tacaatgagg aatggaatgc
    11881 tataaagcat tgcattaatg gaaaaagcat ttaagatttg atgaaaaagg aagcttgccg
    11941 gtaatgaaat aattaagatc tggaacaaaa tgagcaaatt ccattttacc tggatactgt
    12001 tcaaagctct aactgctgcc ttactctctt gcattattgc cccctccgtt tagataaaat
    12061 tcgctcacca attatcagag aaaatactgc cagccaaaat gggtaactaa aatctgtctg
    12121 tcgcgaaact ggtccagcca ccatgttttg tttttcaatt tcctgagtaa attgcctata
    12181 gtctgaattt tgtaatttct ccggagaaac atccccacca aaaaataatt cgaacacagt
    12241 tcttagaaat tcttcattat aaatagcttc aggggtaatc cttcccatgg tttcataaaa
    12301 gttaacttgt gatttaataa tcgaaatcgg accagttata ttattagttt tcaaaggatt
    12361 ataattaata actcctggag attttagatc tttgccgggc tccggaccta tccaaattac
    12421 gtaagtattc atcagtgaat cttctgaaaa actttcggga gcgtagatgc ccttaaattt
    12481 gaaattaaat tccggctgcc tggcaatggc ttccaaaacc cttacccact cctcaacttc
    12541 cttatcgaaa ccaggttccg ttacaataat tgcaaatttt gaatttactt cacccaattc
    12601 gccggttttg aaacttccgg catctattat ttccgtgcga cttatcagat tgttgccttg
    12661 caggtactga ccgataagta aaaccgaatc tttactgaat tgttttttga ctatcgggaa
    12721 ctctcctgga gacgatggga tttgatacca ggttaactca aagctaaatt ttgggaaagc
    12781 tcccgcaata ttcttaaggt atccatgact aaaaagtacg accttgccat acctgtttag
    12841 cccttccaat tcttccaacg caaggaataa attattcctc aaaaagacag agtcatcaat
    12901 gctgtttaca attttattat tatgtaaata tctgatatcg taagcattat aatcagttgt
    12961 atcaagtttt aaattaataa gattcattcc cgcaacttcc gaatcgatta acaacagatt
    13021 ccgtttaacc aacgtttgat tactttgaat tacagggtta gccaggataa aaaccagcgc
    13081 tgcaaagatt agtgatcgta aggctaaaag tgtatactcg tttaagcgaa tgctacgaga
    13141 ccgcgttttt tctactttct ttatccatga gatattgccc acccagattg ttgtgccttt
    13201 gcttttgttt aacaaatgaa ttatcaatgg aaatgttagc gcaagcaaac cccaaaatgc
    13261 ggcaggattt agaaagatga gattcatact agtcgctttc gaatttgaag gaaaccggac
    13321 aatagcgtcg ggagatcatc ccccgaattg aattggtgat aatctatttc ttttgcaagg
    13381 aaagtagtct ggaaatgatt ttcagcaatc cgcttttttt caacaaaaaa ttccctggct
    13441 ttatccgcat cagttaacac ttcctcgcct gtttctaaat ctttgaaagt gtgaaagcct
    13501 tcataggcga attcaatttc attctgagca accatgttta gaaccaatac ttcatttctt
    13561 tgagttttta atgaggtgat aaaatccatt aactcatttt ctttttcata catatcggat
    13621 aagaatataa ccatctcttt acgccccgga tagtaaatgt cctctaagcg agccggcctt
    13681 atcggccatg caccagctgt tttcaattga atgagcattt gcagtaatct ggtgaattgc
    13741 tgtggatgaa ctgttggaga aaggcggctg atgttttcat tatttatggt aaacaaacca
    13801 aaactatcac cctgatttcg tgccaggtaa gctaatgaag ctacaattat ccgggccagg
    13861 tcaatttttc gaaggccacc actttcgtgt tccatagatt tgctggcatc aataataaac
    13921 cgaattgtga tgtgcgtatc aatctctgat tgcctgatgt aatacctttc tgaccgggca
    13981 tacattttcc agtcgagtaa gcggagatca tctccgggtt cataacttct gtattggcta
    14041 aactcctgcc ccattcccag tcggtggctg cggttatttc cgtgaaagaa ttcttcaaca
    14101 actattctgg caaccaattc cagcccttcg atagaactta ttatttcggg tcgaagaaga
    14161 tcggatattt tttcattcga ttctttaagc tttgccattc attggaagcc ggatagtatc
    14221 cagcaatttt tctgtaacca tatcaggttg aatttgggca gattctgctt taaagttaag
    14281 aatgatacga tggcgcagta ccggataagc gacgtgtctg atatcatcaa cagtaactgc
    14341 aaagcgcgcg ttgagcaatg ctcttgcttt agcagtaaga ataattgcct ggcctgcacg
    14401 cggacctgca ccccaccgta cccactcttt cacatattct gaaatcgtat tatcagggcg
    14461 tgttgcacgg actaatttat ttacatattc caccaggtca tcactaatag gaacctgacg
    14521 gacaagattt tgaatttcga taatatcatt cccatttata accctgctca cctccgccgt
    14581 taccgtaccc gtggtttgct ccaggactgt tttttcttct gattctcccg gataaccaat
    14641 ccggatataa agaaggaacc tatccagttg ggcttccgga agaggaaaag taccactctg
    14701 ctcgatggga ttttgagtgg caagaataaa gaacggttta tcaagggtgt aggtcttgcc
    14761 accataggta acttcttttt cctgcatcgc ctccaataag gcggattgag ttttgggtgg
    14821 tgttctgttt atttcatcgg caagaataat attggcgaaa ataggtccct tgttaaactt
    14881 gaagaacttc tttccggtgg catgatcttc ttcaagaatt tcagtgccga gaatatcact
    14941 gggcattaaa tcaggagtaa actgaatcct tctgaaagac aaatcaagac actttgcaag
    15001 ggtacgaatc atcaatgtct tagccaaacc cggaactccc tccaacagtg catgtccgtt
    15061 ggccataaat gtaataagaa ggtggtcaat ggtttcttcc tgaccaataa taactttccc
    15121 aatttccttt tttaattgcg gcaatttatt aatcaaagcc agcactcttt cttttgtctc
    15181 ttccattttt atgatgtcaa ggcataaata accagattca cgccaaattt tgtattatcg
    15241 ttcttatacc attttttgtt tctgaaatcg taatcccatt cgcatccgta gtctttgttg
    15301 ctataaagta cgccaattct tgtattgatt tccactgctt tcaaataatc atgaaccagg
    15361 tcatcgcccc aaccatttaa ttcctgggaa gtagtgggtg gtcccttttc aaacctaaaa
    15421 aagcaattat atatttgatg attatcaggc agctttctca atatgccttt accaaatatt
    15481 cgaagcattt gttcctcaaa tgatttggcg aagagtccgt caatatcatg attgcagtcg
    15541 tctgcaaaaa taaatcctcc attttcaacg tatcgtttaa aatttttcga ttccttgtca
    15601 gtaaactgta ctaatttgtg accgcttaag taacaaaacg gagaatagaa aatttcatca
    15661 cttgacaagg gaattacttt ctcttcggta tcaacttcaa gagttgtata ctctattaat
    15721 gaattcagaa tattcgacgg cattctttga tccgtatccc aatcacctga ctcatattga
    15781 agtctggtga aaaaaaactt cttcctcagc cctattattt tcaattcgaa tcaaaattga
    15841 tttacctggt aatttgcaga aagccggatt acatgaacgg tcgtttctaa aaaaatatcc
    15901 tccaaaataa aggaggatat ttcatcaaaa acaattccta aatcaaattc taaactgcat
    15961 ccgacaaact tgtaaaggta aagtcacgta ccttcatggc aggaatgaga tttccgttta
    16021 ctcgttgctg cttacccagg gcttccaggt tattaagcat aatgacagga ctttcgttaa
    16081 atctgaagtt cttaatggga tgtttaattt taccattttc gataaagaaa gttccatctc
    16141 ttgtcaatcc cgtatatagc aaagtttgtg gatcaacagt acggatatac caaaaccggg
    16201 tcacaagaat tccccgtgtt gtatctttga taagatcctc cagggatgca tctccaccaa
    16261 ccattattcc attacttggc cctggtaccg gctcaacacc tttttgttcg gcccagtatc
    16321 ggctatagaa gagattcttc actattccgt tttcaagcca catcgtttta ttcagtggtt
    16381 ctccatttcc attccaggtg gcagcgggca cttcaggatg cagtggatcg ctgtatatgg
    16441 ttacccgctc atccacaatt ttctcacctt tttttgttcc tcctccttct ttagcgagaa
    16501 aacttctgcc ttcatccgca ttccgggcat tgaaattgaa aagcatattc tgtagcagat
    16561 catgtgcggc ggcgggttcc aggataaccg tatatttacc tggttcgatt gcctgagctt
    16621 cttttgaaag taatgccttg tcaatagcaa tctttgaggc ttcttcagaa ttaaatttat
    16681 ttacatcatt ataatccctg gttacccatc ctgaacctgt tccgtcattg gatcgcattg
    16741 ttactgtaaa atctatgttt gttctccggt tatacgcgaa taaacctttc gaattcatca
    16801 tggaattcat gcttacagaa tcattgaaaa aacccgccgc tgttatgtca cttgttcgcg
    16861 ccggaccaat acttgattca gccactttcg ttcggtactc agcggatatt tttgcagtgg
    16921 attcaacaaa ggatttactc tcagcgaatt cctgcggacc taaaataggt acgaattcag
    16981 gattttccgg agataattga gccagttcct ccgaccttcg gattactttc tccagggatg
    17041 catcatccaa ctcattaatg gttgcgattc cggttttctt tccaaaactg gaccctgcca
    17101 ccaattgaat attggattga tatccgctcg tgcttactgt atttctcgca taccttatat
    17161 tgccttcatc ggtagaaccg agatttactt cacattcatc cgccttagaa aagcttaacg
    17221 ctttttgcag aatctgttgt gcttcgtctt ttgttaatat tgccattttt tttattctta
    17281 aatttgataa tttgattaaa tggtcctacc ggtatttatg acattaacac cgttaaatct
    17341 ggaagttgaa cagccatggg aaaccgcgct aacctgagaa ggctgtcctt taccatcgaa
    17401 gaagctgcca aagaaacgat aatcactttc atcacaaatc ttcacgcagg aattccaaaa
    17461 ctcctgtgta ttagattgat aagctacatc attcaacatg ccttttattt cgccattttc
    17521 aatttcgtag aataaagttc caccaaactg gaaattatac cgctgctggt caatactgta
    17581 agaaccgcgg ccaatgatgt aaataccttt ctctacatcc ttaaccatat ccatagaaga
    17641 atacttctcg cttcctgatt tcagagacac gttcggcatt ctttggaatt gtacgtcatt
    17701 ccagctttgt gcataacagc aaccatgcga cgatttttca cccagaatat gaacttgatc
    17761 ccgaatggcc tgataattga ccaggatgcc ttcctttacc agatcccatt ccttgcactt
    17821 aactccctcg tcatcatatc ctacggcacc cagacttcct acctgggttt tatcagctat
    17881 gaaattcact aatttacttc cgtagttaaa tgattttgat tcccattttt ccatggtagc
    17941 aaaacttgtt cccgcgtaat tcgcttcata gccaaggaca cgatccaatt ccagcggatg
    18001 acctacactt tcgtggatgg ttaacccgag atggttggga tccagaacca aatcaaactt
    18061 ccctgcttcg accgacttgg cagtaagcat ttcttttgcc tgtttagcgg ctgctgtggc
    18121 gtcttcaatt atatcatagt atttactgta atgaattaag ccacccggtc cttccacttt
    18181 acctgaagca tcgccactca tgtattcata acccattccc atgggagcac tcagcgactg
    18241 tcttgtctta aatttaccgg cagccctgtc aacagcagtt acattaaatg tcggccagat
    18301 gcggtgaaca tcctgatcaa tataagaacc gtctgaagag gcgaaatatt tttgctcatt
    18361 taccatgaaa agcccggagt taacaaaact cgccccattt tccatagcgg aagcatttgc
    18421 agaaagaagt agttctactt tttctgaaac cgggacttcg aagcagtttt tctcaatggg
    18481 agttttccag gcaacctcac catgactttg agcaggcgcc aaaataacag gttccttttg
    18541 aattctacta ttcgcttttg caatagaaac cgccaattca gcagctttct tgatggattc
    18601 gggagaaaca tcatgagtgg atgcaaatcc ccaggttcca ttggcaatta cccgcactcc
    18661 tattccaaag gattcggtgc ttaccacgtt ctgaactttg tcttcacgtg taaatacgaa
    18721 ttggtttaag tagcgaccaa tccgtgtgtc aacataagtt gcccctaacg cctttgcgga
    18781 attcagggca atatcggcca gctttttttt ggctgtgaca tccaaaaatg gtgtcagcaa
    18841 tacctcttct gatacaggag tgccgagtat gggtataggt agcattgcag caccaactcc
    18901 aagtcctgtc cattgaataa attctctgcg tttcaagatt cttagtgttt agtttgattt
    18961 tagacctatt cgtaaaaatg agtcttgatt tctattattt tgcaagattt aagttagttt
    19021 aagcggatct caatgacaaa ttaatctttt ataaacggcg tcataacagg ctgttcagtt
    19081 agtttttaac aatgtttaag aaaatcatcc atattgacat ggatgcattc tatgcatcgg
    19141 tggaacaacg ggacaacccc aaccttgcca atttgccggt tgcagtgggc tgggattcat
    19201 ccagaggcgt tattgcaacg gcaagttatg aagcacgaaa atttggggtt cgatccgcga
    19261 tgctagtttc cgccggtaaa aaactctgcc cggaattgat aattgttgaa ccaagaatgg
    19321 atgcttataa aaatgcttct gtccagattc ggtccatctt tgatgaatat acagatctgg
    19381 ttgaacccct atcccttgac gaggcgtatc tggatgtaac taacaataag ctaaatctgc
    19441 gtagtgccac aatcattgca agagaaatca aaagaaaaat caagaaccaa acccatttaa
    19501 ctgcttcagc cggtatttca atcaacaagt ttctggcaaa aatagcgtcg gacatgaata
    19561 agccggacgg actaacccta attcacccaa acaaagcttt agaattccta aagcaactga
    19621 aaatagaaaa atttcatgga atcggaaaag tcactgcgcg aaggatgaga aattttggaa
    19681 tatcgaaggg aatagacatt atccattccg gggaagaatt tatgttgaaa aatttcggca
    19741 agaacgggaa gtttttcttc cagattgcac aaggaataga caacagagag gtgagcccgg
    19801 caagggagcg gaaatcggtt agcgttgaaa atacttttgc cgaggatctg acgatcacag
    19861 aagacatcca ttatgaatta agaaagttag cttatgtatt agccaaaagg atccatcgaa
    19921 atcaatttat aagtaaaact ttaactctaa agattaaatt ttctgatttc aagataatta
    19981 acagaagtaa gacaggggat aatttgattg ctgatgaaca acaaatcaga gaacttggtg
    20041 agtcaatgtt ggatcatatt caaattgaaa aaggtgtacg gttattggga cttggagtga
    20101 ccaaacccgg aggtaataaa gaagatagcg caaattctcc cagtcaattg acccttgact
    20161 tctaggaaac tttactgaac ttactgtaga caaaagcggt agctccgccc accagggcac
    20221 caaaaaggtg agattcccaa cttattccat attgtccggg gaaaatccca tatacaagag
    20281 tccagtacac ggataaaatc acaatgctta taaatataga tttcagatct ttcctgaaca
    20341 gtccaaagaa aaccaggaaa ccggccagcg cgtatataac tccgctggcc ccaatatgga
    20401 aggattcacg gccaaaaatc caaaccagta ctccggttcc aaggtaacat tggatgaaaa
    20461 ctttgcccgc gattcttgaa tagaagaaat ataaagttgt gccaagaaac agtagcggaa
    20521 aggtatttga ggcaagatgc ataagatttc catgaatcag aggaccggta ataactccta
    20581 tcaaaccaaa ccaggttctt gggtaaatcc caaagattcc taaatcaaag ttgacatatt
    20641 tctctccggt ataaataagc cacatgaaga aaaccatgcg ggaagggacg ataattgctt
    20701 tactaatggt agcttccccc attctatagc tgcgatgggt ctttaatcat tttgagatca
    20761 atcactcttg attcctcata tccaacggac ataaagccga aaacgccgcc agatgactcc
    20821 gcgatcctta acgcgatttc tatcaggctc tcataaaatc ttatgctaaa attcttatcc
    20881 agctttggaa agattgaatt caacttcttc aattcatcgg taatcatatc cgtacgctta
    20941 atagtatttg aaggaaactc atgcagcaag accttcaatt tgtcttcaac gtcttcagca
    21001 acaagagtgt aataatcaac caattccttc cttgatttct gaggtctcag cgttataata
    21061 tttaccgctt ccttcagctc ggacctgtca actgtatcgt cagctccggc tactaatatg
    21121 gcagtgagca atggggcttt aaacatcagg tcaacctcat cattattaag tttctcaaat
    21181 tcaggaatca taatcaggta agtttacttc aaagtaataa aatacgttgt ttgtaatgaa
    21241 ttttgaacaa ttttatcttt ttttttgaac agttttcaag gaattaacca ataaattagg
    21301 gtaacattat tcttatggaa gagccattac cgtttaagat ttttgtaatc gaagatgatc
    21361 caacctatcg aaagtttttg gagtacgttt tgagtttaaa tccggacttc gaggttagca
    21421 cgtttgagaa tggtaaaagt ttcatagaaa gccttgataa gaatcccagc ctggtaaccc
    21481 tggactactc ccttccggat attaaagggg aggatgtact atcttatata aaaaaataca
    21541 atcctgatat acctgtgata gtaatttctg cacaggagaa gattggaaca gcagtgagtc
    21601 ttctcaaact tggcgctttt gattacattg taaaggatga tgaaacgaaa gacaaactac
    21661 ttaacacaat aaataacgct cgaagaaata tttccctgat caccgaaata gtcagtttaa
    21721 gaagagagat atctgagaaa tatgagttca gcaaaagtat tatcggaaat agtaaggcaa
    21781 ttaaagatgt ttttaagtta ttggaaaaag ctgtttccac taacatcact gtttccatta
    21841 ctggagaaac cggaactgga aaggaattgg tggccaaggc aattcattac aattcagcca
    21901 gaaagaacaa gaaatttgta gccgtgaata ttgccgccat accaaaagaa ttaattgaaa
    21961 gtgaattatt tggacatgaa aagggagcct ttacaggtgc ggcgtcgcgc aggctgggga
    22021 gatttgaaga tgccaatcga ggtacaattt ttctggatga aattggagaa atggatatta
    22081 atcttcaagc aaagctattg agagttttgc aggaaaagga aatttccagg attggcggga
    22141 atgaagtgat taaacttgat gtaagagtga tagtggcaac ccacaaggat ctcccggagg
    22201 aagttagtaa aggcaatttt cgggaagact tattttacag actccttggc cttcctgtac
    22261 atttacctcc cctgcgggaa cgcgataatg atataattct tattgcaaaa tcattgctga
    22321 gaaatttctg taaagaaaac aggattcccg agatccaaat tagcgtggcg gcacaggaaa
    22381 aattgatgca atattctttt ccgggaaatg tacgcgaact gaaatcaatt attgagttag
    22441 ccgcagttat gtgtaatggg gaatccataa attctgatga tcttaaattt ggcaacctgg
    22501 cgcgtctgga aaaattactc actgaggaac tcactcttag ggagctcaat attaaagtga
    22561 ttaaatatta tcttgaaaaa tatgacagca acgtaaaact tgttgccgat aaactgggta
    22621 ttggaaaatc aagtatttac cgttacctca ataatgaaca atgataattt atgaaggctg
    22681 atagcatttc aatccggaat tatattaata agggtagttt ctaccgggca gtagttgaag
    22741 atggtgcaga tataattttc atagtagatt acgagggcat tatttattat cacaatccat
    22801 ccgtaaaaaa ttttttagga tacagtgatg gggaattaat tgacaagaac tttattgagc
    22861 aaaatcatcc tgagtacagg tccagattaa cgaagacgtt caaagaaatt tcaaaagaga
    22921 aatatgcgga caatatagaa ttttatttta gaaagaagaa cggtgaatat acatacctcg
    22981 aattcaattc cataaatctt caccataagg accagataga tgccttgctg cttgattgca
    23041 gggatatttc acaaaggaag aaagacgcgg aagaacttat taaagctcaa aaggcaaaag
    23101 aattatttct tgctaacatg agtcacgaaa ttcgcacgcc aatcagcggg attgcaggca
    23161 tggtaacttt attggaacaa tttgacagga acccggagga aatgaaatat ttaaatgcga
    23221 ttaagcattc agccgagcat cttaaaatca ttataaatga tattttggat tttgcagcca
    23281 tagaagcagg aaaattaacc tttgaaaata ttggtttcag accttatgag caagccggga
    23341 atgtggtgaa ctcctttgaa tataacctaa aagataaggg acttgaaatt gatttcagtt
    23401 ttgatggaga aaaagattta atagtgaagg gggaccctgt aaggttaaat caggttctgg
    23461 ttaatttaat aaacaacgcg ataaaattta ctcctcaggg aggagttaat ctaagtgtaa
    23521 aaattagtaa gcaaggtgaa aagtatttgg ccacttattg tgtaaaggac acgggaattg
    23581 gaattccgca taataaaaga atgaacctgt tcgaatcatt ttcgcaggca gatacctcga
    23641 ttaacagaag atttggaggt acagggctcg gtcttgctat tagcaagcat attgtaaata
    23701 ttcagggtgg ccggatatgg tttgaaagtg aagttgatca gggaagcaca ttttgttttg
    23761 aaattccatt tgaacccggt aatgtatctg atttggttac tgaaaagaaa gaaaaaaaat
    23821 ctgatgaaac gagtctggat gggttacggg tattgttggc tgaagacaac gaaataaaca
    23881 aactctacac aaccaaggtt ttatccgggt taaattgtaa ggttgaaacg gttgaaaatg
    23941 gcaaagattg cgttgatctt ctggagaaaa ataacttcga tttagttctg atggaccttc
    24001 agatgccaat aatggatggc tttgaggcca ccaaaataat cagggaaaaa ctccctaatc
    24061 caaagaaaga aataaccatc attgctctca ctgcaaacgc gataaaaggt gacaatgaaa
    24121 aatgtattgc tgctggaatg aatgattatc tttctaaacc cttcacacca gaaaacttaa
    24181 agaaaaaaat tttaagatgg gttaaacgcc ctggtcattc tgaaattaca aaggcaagca
    24241 gtgattcggt tattgatttg acctatttga ggacggtttg taataatgat gaatcattcc
    24301 tgaaagaaat gatcaccaca ttccgaaatt cgactccgga actccttgat caaatggaat
    24361 ctgcattgtc acaagatgat atcataactg tttctaaaat tgcccataaa ataaaaccat
    24421 cgatctcatt tatgggcata caatctgcat tgcaaggagt gagtgaaata gaaaatacta
    24481 aagctgctcc gctcccttcc ggttataatc aaatggtgtt ggattttctt aaagaaatca
    24541 ggaaagcgtt aattgaactt gacacaatta ggtaattcta cctgtgcata actgctattt
    24601 tcttgtaggt aaatgggcgg ttttcaacgc caattacaag gaaatagatt ccattgttaa
    24661 tttctgatac ctccatctca aacaggtttc ctttcaattc tacatcaccc tggttgatgg
    24721 taacacctct ctgatccaca atcttatacg tgtaattata aggcccggga tttggaattt
    24781 caacattcaa atactggtca accggatttg gataaatatt cgcttggttc aacctggatt
    24841 ttatgtcttc ctccaaacca acaattagcg gcggcatctt atcaccgagt tgagtaataa
    24901 tggtttgata tatctccctc gtcgcccggt tttgaataaa tgtgattacc gccagttttg
    24961 tggcatcaga tattttcacg tctattggcc aggtcaaatc aaatgttgct gatcctccgg
    25021 tattccagtc ttgtgtatat aatgtaccag cagcatctgg cagcaacttt cggaggacat
    25081 tataataaac agttccacta ccattaagta tttcttcctc cacaatcgcg ttatgcacca
    25141 ctaattgctg accaacaagg tcatttctgg aagtaatcgt tgtgcggata cttatatcac
    25201 tttctacagc gctcaattct gttacggcta tatcaaactg cggatcaatc agcgcacgct
    25261 tattgatatc aatatctgtt gca
//
LOCUS       DASWHX010000005         8376 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_3806, whole genome shotgun sequence.
ACCESSION   DASWHX010000005 DASWHX010000000
VERSION     DASWHX010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 8376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8376
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3806"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..247
                     /locus_tag="VGA21_00180"
     CDS             <1..247
                     /locus_tag="VGA21_00180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9648946.1"
                     /translation="FFSWNFFDAILQQKEYFSPYIFEELAPGILNENPDLKNNFEERI
                     KADSVFANSPFQQLNFIYQNSKYREKDFMRYPVYKIK"
     gene            complement(248..1897)
                     /locus_tag="VGA21_00185"
     CDS             complement(248..1897)
                     /locus_tag="VGA21_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019596140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX9648947.1"
                     /translation="MKNLIQNSFLIIFFLLLISFQSSNEETDPIAAAETLIGLEFTQA
                     ERDSLRDGLEDLKESYRAMREYPLANSNMPAILFNPIPPGFIIPDKQEKIDWKFNDAE
                     IPDNKNDLAFYTVAQLSELIKTKKISSLELTTFFLNRLKKYGDTLQCVITLTEDLALN
                     LARKADQEIASGKYKGPLHGIPYGVKDLLAVKGYKTTWGARPYEEQILDETSTVVAKL
                     TESGAVLIAKLSMGALAWGDVWFGGVTRNPWNLNQGSSGSSAGSASATAAGLVPFAIG
                     TETWGSIVSPSTRCGTTGLRPTYGRVSRYGAMALSWSMDKIGPICRTAEDCAIVFQAI
                     QGTDGIDPTLYTSVFNYKQNVDLSKLKIGYFKDLFDRDYNSKSADSMSLEVLKKLGAK
                     LIATKMPEGIPISSLGIILNAEAAAAFDELTRSNRDSLLVRQIKNAWPNSFRRSRFIP
                     AVEYIQANRIRTDLIAAMHKLMSGFDVIVTPSFGGPQLLATNLTGHPVVVLPNGFNER
                     GSPVSISFLGNLFDEATILAVAKAYQEATDFDEKHPEMFKK"
     gene            1957..2424
                     /locus_tag="VGA21_00190"
     CDS             1957..2424
                     /locus_tag="VGA21_00190"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX9648948.1"
                     /translation="MLDRKSQFLHDWEYDHWANERYLKFIEENQISNERVSRICGHVL
                     VAQVLWIKRIIPVEMDDYSLWENYSLIEMNKMSETCYSVWKNFLEEYPDNAFSEIISY
                     TNTQGLAFKTPVNEIISHVLYHGAYHRGQLAILIRDLGFTPPYTDYVAFLRGQ"
     gene            2497..5289
                     /gene="polA"
                     /locus_tag="VGA21_00195"
     CDS             2497..5289
                     /gene="polA"
                     /locus_tag="VGA21_00195"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014020130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEX9648949.1"
                     /translation="MSKSPKTLFLLDAYALIYRAHFVFSNPSTKYNPINSRGMNTGVP
                     YGFTNSLLEILQKQKPSHIAVVFDTSAPTFRHEEYEEYKATRQETPEDIKTGIPWVKE
                     IVNGFNIKILEMDGYEADDIIGTIAKKAAKKGFEVFMMTPDKDFSQLVNENIYLYKPA
                     YMGNAVDILGPEEVKKKWEIKEVDQVRDILGLMGDSVDNIPGIPGVGPKTAVKFIEQY
                     GSIEELIKHTEDLKGKQRENVEKYADQAILSKRLATINTEVPVDFIEDDFVYREPDAE
                     RLKTVLRELEFRTLFSRVFSEKEAKIPVASGQISMFESGDNDENTKTEGRQFTNISEE
                     TSSYNLITSEADRKSLISKLLKQKEIAFVTLSEGSDPMLNSLIAFSISFKNGEASYVP
                     FPDDEKKSVQLLSEFKKVFESPSILKIGHDVKFDMILFRKLGVELAEPFFDLKLAHYL
                     VDPETSHELNVLSENYLQYFSMPLVDLTESGKKPGDLRDTIPEKLKDFCCEKADLIFQ
                     LKKVLQDELKKEKVEKLYKEVEHPLLRVLAEMEFEGVKIDENTLGEMSADFEKKSAKV
                     QKEIFDLAGEEFNIGSPKQLGDILFEKLELMDNPKKTKTGQYATGEEILNKLASQHKI
                     VNKILDFREYQKLKSTYLDALPLMVNPYDNRIHTNYSQTVAATGRLSSINPNLQNIPI
                     RSEKGRAIRKAFVPRDKNYVLLSADYSQIELRIMASFSEDKEMIKAFNEGKDIHAITA
                     SKIFKMPLEKVDADMRRMAKSANFGMIYGISAFGLSQNLNIPRKEAADIIESYFKEFK
                     AVKNYMDGVITDAKKKEYVETILGRRRYLRDINSRNAATRGYAERNAINAPIQGSAAD
                     MIKIAMVKIQQWMNKNKLKSKMIMQVHDELVFDAHKDEVENLRMHVVELMQNAIKMKV
                     PIEVETGIGKNWLEAH"
     gene            5315..6262
                     /locus_tag="VGA21_00200"
     CDS             5315..6262
                     /locus_tag="VGA21_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009578511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEX9648950.1"
                     /translation="MTSIPTLEDIKLAAEKIRPYVRRTPVFTSQTFNNWTGSKIFFKT
                     ENFQKGGAFKFRGACNTVLSLTDEEKKMGVTTHSSGNHAQALALVSKMVGIKAYIVMP
                     SNAPKVKKYAVLGYGAEVIECEPTLQAREANVKKIIAEKGSVLVHPYNDYRIIAGQAT
                     CAMEFIEEIRDLEIMVAPVGGGGLLAGTSLTCNYISDKIDVFAGEPEGANDAFLSLQK
                     GEIVPSLNPDTIADGLLTSLGDKTFPIIQKHVTEIITVSDNEILDAMKKVWERMKIII
                     EPSSAVPVAAVLKNPDKFTGKNVGIILSGGNVDLENIRF"
     gene            complement(6265..7206)
                     /locus_tag="VGA21_00205"
     CDS             complement(6265..7206)
                     /locus_tag="VGA21_00205"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG-like jellyroll fold domain-containing
                     protein"
                     /protein_id="HEX9648951.1"
                     /translation="MRRKHQKLIIGIALALLMLTGCKKDEENTPYLVSLAGLIRYEVS
                     IMGVSFNPDLAENRVFLSNVEAEVTSAEPTLIKFIVPDLPWGIYEVTLISGDAIYVIG
                     NYTILFPVDPIGLGAYYPLNGSAVDLGPLSHRGTEVGDLIYEMDRHGEEGLAASFDGV
                     DDVITTSATLDYEFRTVSFWFYPTNVMKTEKQVIISLDDNVLQYGRVRAEIQAGTLTL
                     WAGGLVSTYSMPVQNNTWYHIALARDPTATGYFINGILVVLGEADGEGETVTPNDNLV
                     IGAGPAESAEYFEGIIDEIIVFNRVLSEPEVEFLGEY"
     gene            7362..7997
                     /locus_tag="VGA21_00210"
     CDS             7362..7997
                     /locus_tag="VGA21_00210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9648952.1"
                     /translation="MNRILISLLICLIPLISWAQIIIATSNGNQLKLTSEGEWEEVWE
                     TLPNQAEYNSFTLPEESRALLKTNNAKFGIQFNSEKWDVLSGSHFPGSGFENFEFEFS
                     HRKSLAIGLLMTDPYPASIEKVISRSLNQIKETATELTVAYGEVFSVNKKTVYMVILQ
                     TVEHGIPLTYYNYYYSGDEGSVQFLGYCNQKVFWKYEDDIKDLLNGFLVLN"
     gene            8041..>8376
                     /locus_tag="VGA21_00215"
     CDS             8041..>8376
                     /locus_tag="VGA21_00215"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX9648953.1"
                     /translation="MPKHTFNNLKKEKKEIFLKEALEEFSLKGYYGASLTQLVKKLQM
                     AKGSLYQYFDDKDEMYAFLIAEACSRRLPFFNALDGKPREDYSFNYLIISLKFDVTYP
                     YIASLFYNGF"
BASE COUNT         2612 a         1636 c         1717 g         2411 t
ORIGIN      
        1 ttttttttcc tggaactttt ttgatgctat tcttcaacaa aaagaatatt tttcccctta
       61 tatttttgaa gaacttgccc cgggaattct gaatgaaaat cctgatttga agaacaattt
      121 tgaggaaaga attaaggctg actctgtctt tgctaacagt ccatttcaac aactcaattt
      181 catttatcaa aactcaaaat acagggaaaa agattttatg agatatccgg tttacaaaat
      241 aaagtagtta tttcttaaac atctcagggt gtttctcatc aaaatctgtt gcttcctggt
      301 aagctttggc tacagcaagg attgtggctt catcaaatag atttcctaga aaactgatgc
      361 taaccggact acccctttca ttaaaaccgt ttggtaaaac tacgaccgga tggccggtaa
      421 gatttgttgc cagcaattgc ggcccaccga aacttggcgt aacaattaca tcaaacccgc
      481 tcattaattt gtgcatggcc gcaatcaagt cggtgcggat tcggttagcc tggatatact
      541 ccacggcagg gataaacctt gatctgcgaa atgaatttgg ccaggcattc ttgatttgcc
      601 gcaccagcag gctgtccctg tttgatcggg tgagttcatc aaaagctgcg gcagcttcgg
      661 cattcaaaat tattcccagc gaacttattg gaattccttc gggcattttt gttgcaataa
      721 gtttagctcc taattttttc agcacttcca gagacattga atccgcggat ttggaattgt
      781 aatcccgatc aaataaatct ttaaagtacc caattttcaa ttttgacaaa tcaacatttt
      841 gtttataatt aaatacactc gtataaagtg tgggatcaat tccatcagtg ccctgaattg
      901 cctgaaaaac tatagcacag tcttcggcag ttcgacaaat gggtccaatc ttatccatgc
      961 tccatgacaa tgccatggcg ccataacggc ttactcttcc ataagttggg cgcaacccgg
     1021 tagtgccaca gcgggtggag ggggaaacaa tcgagcccca ggtttcggtt ccaattgcga
     1081 atggaactaa tccggcagcg gttgcggaag ctgatccggc tgatgaacca cttgaaccct
     1141 gatttaaatt ccatggattg cgtgttactc caccaaacca aacatcaccc caggccaatg
     1201 cccccatgct taatttagct atcaatactg caccagattc tgttaacttt gctactacag
     1261 ttgatgtttc atctaaaatt tgctcttcat aaggcctggc accccacgtt gtcttataac
     1321 ctttaactgc cagcaaatct tttacaccat aaggtatgcc atgcaaagga cctttgtatt
     1381 tgccgctggc aatttcctga tctgccttgc gggccagatt tagtgccaga tcttctgtga
     1441 gtgtaatgac acattgcaag gtatctccgt atttcttcaa tctatttaaa aagaaagtgg
     1501 ttaattctaa tgaagaaatt ttcttcgtct taatcaattc cgataattgg gccacagtat
     1561 aaaacgccag atcatttttg ttgtctggta tttccgcatc attgaatttc caatctattt
     1621 tttcctgctt atccgggatt ataaatcctg gcgggatagg attaaataag atggccggca
     1681 tatttgaatt agcaagtggg tattcccgca tcgcccggta agattccttc agatcttcca
     1741 ggccgtcgcg caaagaatcg cgctcggctt gggtaaactc aagaccgatg agtgtttcag
     1801 ctgccgcgat aggatcagtt tcctcattcg aggattgaaa tgaaattaac agtaagaaaa
     1861 aaataatcag aaatgaattt tgaatcaggt ttttcatgag aatcagggtt gaagattatt
     1921 aaagataccg gaattttttg ttaattgtaa tgaaatatgt tagacaggaa atcacaattt
     1981 ctgcatgatt gggagtatga tcattgggca aacgagcggt atcttaagtt cattgaggag
     2041 aatcaaattt caaatgaacg ggtgagcagg atttgcggtc atgtcctggt cgcccaggtt
     2101 ctctggataa agcgaattat tccggtggag atggatgatt attcactctg ggaaaattat
     2161 tctcttattg aaatgaataa aatgagtgaa acctgctact cagtatggaa aaattttctt
     2221 gaagaatatc ctgataatgc attttctgaa attatttcat atacaaacac tcagggtttg
     2281 gcatttaaaa ctccggtgaa tgaaataatc agtcatgttt tatatcatgg agcttaccac
     2341 cggggacagt tggcgattct tattcgcgat ttaggtttca ctccccccta taccgattat
     2401 gttgcttttt tgagaggtca gtaattttgc ggtttttttt gaatcaaaag agctggtatt
     2461 cttcctcttt gactctgttt ttctaccttt atgttcatgt ctaaatcccc aaaaacgcta
     2521 ttcttacttg atgcctacgc cctgatttac agggcgcatt tcgttttcag taatccctct
     2581 accaaataca accccatcaa ttccagggga atgaacaccg gtgttcccta cggtttcact
     2641 aattcgttac tggaaattct gcagaaacaa aaaccgtcgc acatcgcggt agttttcgat
     2701 acatccgctc caactttccg tcatgaagaa tatgaagaat acaaggcaac ccggcaagag
     2761 acccctgaag atattaagac cggtattccc tgggttaagg aaattgtgaa tggtttcaat
     2821 ataaaaattt tggaaatgga tggttatgaa gcggatgata ttatcggaac cattgcaaaa
     2881 aaagccgcca aaaaaggttt tgaagttttt atgatgacac ctgataagga tttctcccaa
     2941 ctcgttaatg agaatattta tctctataag cctgcttata tgggaaatgc cgttgatatt
     3001 ttgggccccg aagaagtaaa gaagaagtgg gaaattaaag aggtggatca ggtgcgcgat
     3061 attttagggt taatgggaga ttccgttgac aatattcccg gaataccggg agtgggcccg
     3121 aagacagccg tgaagtttat tgaacagtat ggatctattg aagaacttat aaaacacacg
     3181 gaagatctga aagggaagca aagagaaaat gtagaaaaat atgctgatca agccatatta
     3241 tcgaaacggt tagccactat aaatactgaa gttccagtgg atttcatcga agacgacttt
     3301 gtttatcggg agcctgatgc ggaaagatta aaaactgttt tgcgtgaact ggaattccga
     3361 acgttgtttt cgcgggtatt tagtgaaaag gaagcaaaaa ttccggtagc gtccggacaa
     3421 atatcaatgt ttgaatcagg tgataatgat gaaaacacaa aaaccgaagg cagacagttt
     3481 accaatatat cagaagaaac atccagttat aatttaatta cgtccgaagc tgatcgaaaa
     3541 agtctgattt cgaaactgct taaacagaaa gaaatagcgt ttgtcacact ttctgaagga
     3601 tctgatccta tgttaaattc actgattgca ttctccatct catttaagaa tggcgaggcc
     3661 agttatgttc cttttccgga tgatgagaag aaatcggttc aattgttatc tgagtttaaa
     3721 aaagtttttg agtctccttc aattttgaag attggtcatg atgtgaaatt tgatatgatc
     3781 ctgttcagga aattgggagt tgagctagcg gaaccatttt ttgatttaaa gcttgcccat
     3841 tacctggttg atccggaaac aagtcacgag ttaaatgtac tcagtgaaaa ttacttgcag
     3901 tatttttcta tgccgctggt tgaccttacc gaaagtggca agaagcccgg tgatttgaga
     3961 gatacaattc cagaaaaact aaaagatttt tgttgcgaaa aggcagattt gatctttcaa
     4021 ctaaaaaaag ttctccagga cgaattgaaa aaagaaaagg tagaaaaatt atacaaagaa
     4081 gttgagcatc cgctattacg ggttttagcg gagatggaat ttgagggagt gaagattgat
     4141 gaaaacacgc tgggtgaaat gtcggccgat tttgagaaga aaagtgcaaa agttcaaaag
     4201 gaaattttcg atctggcggg tgaagaattt aacatcggat ctcctaaaca acttggcgat
     4261 attctgtttg agaagcttga gttaatggat aatccaaaga aaacaaagac cggccaatac
     4321 gcaaccggtg aagaaatact aaataaactg gcatctcagc ataagattgt gaataaaatt
     4381 ctcgacttcc gggaatacca gaaattgaaa tccacctacc tggatgcgtt gcctctaatg
     4441 gtaaatccct acgataaccg aatccatacc aattatagtc aaactgtggc ggctaccggt
     4501 cgtctcagtt caatcaatcc taatttgcag aatattccca ttcgatcaga aaaaggcagg
     4561 gccatccgca aagcttttgt accccgggat aagaattacg ttttattgtc ggcagactac
     4621 agtcagattg aattgagaat aatggcttct ttttctgaag ataaggaaat gataaaagct
     4681 tttaatgaag gaaaagatat tcatgccatt actgcctcaa aaattttcaa aatgccacta
     4741 gaaaaagttg atgccgacat gcgaagaatg gctaagtctg ccaacttcgg gatgatttat
     4801 ggtatctccg catttggatt atcgcaaaat ctaaatattc ccaggaaaga agctgcagat
     4861 atcattgagt cgtattttaa agaattcaaa gcggtaaaga actacatgga tggagttata
     4921 accgatgcca aaaagaagga gtatgtggaa acaattctgg gccggaggcg atatttacga
     4981 gatattaatt cgcgtaatgc cgccacgcgg ggatatgctg agcgcaacgc tattaatgca
     5041 ccaatacagg gcagtgcagc cgatatgatt aagattgcaa tggtgaagat tcagcaatgg
     5101 atgaataaaa acaaattgaa atcgaaaatg atcatgcagg tacacgacga acttgtattt
     5161 gatgcacata aagatgaagt tgaaaacctg agaatgcatg ttgtagaatt gatgcaaaat
     5221 gcaattaaaa tgaaagttcc tattgaagtt gaaaccggta tcggtaagaa ttggctggaa
     5281 gctcattgaa aaaaaaataa tatcattagc gttcatgact agtattccta ctctggaaga
     5341 tataaaattg gcggcagaaa aaatccgtcc ctatgtacgc agaactccgg tgtttaccag
     5401 tcagactttc aacaattgga ccggatcgaa aatttttttt aagactgaaa attttcagaa
     5461 aggcggcgca tttaaattta gaggtgcttg caatactgtc ttgtcattaa cagatgagga
     5521 aaagaaaatg ggggtaacta cacattcctc cggaaaccat gctcaagctc tggcattggt
     5581 ttctaaaatg gtgggcatta aagcttatat agtaatgcct tccaacgcgc caaaagtgaa
     5641 aaagtatgca gtgctgggtt atggagcaga ggtaattgaa tgcgaaccta cgctacaggc
     5701 aagggaagcc aatgtaaaaa aaataattgc tgaaaaaggt tccgttcttg tccatccata
     5761 taatgattac cggataattg ccggccaggc aacttgtgca atggaattca ttgaagagat
     5821 tagagatttg gaaattatgg tggcgccggt tggcggcggc ggtctgctgg cagggactag
     5881 tcttacctgc aattacattt ctgataaaat tgatgttttc gcaggcgaac ccgagggagc
     5941 caatgatgca ttcctgtcct tacaaaaagg cgaaatagtt ccctctctaa atcctgacac
     6001 catcgccgat ggattgctga cttccctggg agacaaaacc ttccctatca ttcaaaagca
     6061 tgttacggaa atcatcacag tttcagataa cgaaattctg gacgctatga aaaaagtgtg
     6121 ggagcgcatg aaaattatta ttgaaccttc ttccgcggtt ccggtcgcgg ccgtactaaa
     6181 gaatcccgat aaattcacgg gaaaaaatgt gggcataatt ctgagcggcg gtaatgtgga
     6241 cctcgaaaat atcagattct gaaatcagta ttctcccagg aattcaacct ctggttcgga
     6301 aagcacccgg ttaaagacta ttatctcatc gataattcct tcaaaatatt ccgcgctttc
     6361 agctggacct gctcctatta ccaggttatc attcggtgta actgtttcac cctcaccatc
     6421 agcttcacca agtacaacca ggatcccatt aatgaaatat cccgtggcag tcggatctct
     6481 cgccaatgca atatgatacc aggtattatt ttgcaccggc attgaatatg tactaactaa
     6541 acctccggcc cacagtgtta gcgtacctgc ctgaatctcg gcccgaactc tcccgtattg
     6601 caatacatta tcatcaagag atataattac ttgcttttct gtcttcatca cattagtcgg
     6661 ataaaaccaa aatgaaacgg ttcgaaattc ataatcaaga gtggcagatg tggtaataac
     6721 atcatcaaca ccatcgaagc tggccgcaag tccttcctca ccgtgccggt ccatttcata
     6781 aattaagtca ccgacttcag ttccacgatg gctcaacggg cccaaatcaa ccgcgcttcc
     6841 attcaacgga tagtatgcac caaggccaat tggatccacc ggaaaaagga tagtgtaatt
     6901 tccaattaca taaatagcat cgccggaaat taaagtgacc tcataaattc cccaaggtag
     6961 atcaggaact ataaacttaa taagcgtagg ttccgcggag gttacttctg cttcaacgtt
     7021 gcttagaaaa actctgtttt ccgccaaatc cggattgaag ctcactccca tgatgctgac
     7081 ttcataacga atcaatccag ccaatgaaac cagataggga gtgttctcct catctttttt
     7141 gcatcccgtg agcattagca acgctagcgc aattccaatt ataagctttt ggtgttttct
     7201 acgcatgaat taacctactt atcaaaattc atcaattcca ctcaaaaagc gtgctggcaa
     7261 atgtcgaatc ttgtaaagaa aatttagtac attgttgtag ttcatcctct atcaatggtt
     7321 taatttttgt tgcctgttgt cagattgcgc aagttcttac aatgaataga attctgatta
     7381 gtctactcat ttgtttgatt ccattgattt cctgggctca gatcatcatt gctacatcca
     7441 atggaaacca attaaagcta acctcagaag gtgaatggga ggaagtctgg gaaactcttc
     7501 caaatcaagc tgaatataat tccttcaccc tgcctgaaga atccagagcc ttgttgaaaa
     7561 caaataatgc gaaattcggg attcagttca atagcgagaa atgggatgtt cttagcggtt
     7621 ctcatttccc aggatcaggg ttcgaaaatt tcgaatttga attctctcac aggaaaagtc
     7681 ttgccattgg attgcttatg actgatccct atcctgcttc catagagaaa gttatttcca
     7741 gaagtctcaa tcaaattaaa gaaactgcta cagaacttac cgttgcctat ggagaggttt
     7801 tcagtgtgaa caagaaaact gtatatatgg taatcctgca gacagttgaa catgggattc
     7861 cacttactta ctataactac tattattccg gtgatgaggg ttccgtgcaa tttcttggat
     7921 attgtaatca gaaggtattc tggaaatatg aagatgatat caaagatctg ctgaatggat
     7981 tcctggtcct aaattagcca aattcaagct aaatacttat tattgtgacc agctagtcac
     8041 atgccaaaac acacattcaa taatctcaaa aaggaaaaga aggaaatctt tctgaaagaa
     8101 gcattagaag aattttccct taagggatat tatggagcca gcctaaccca attggtaaaa
     8161 aaactgcaaa tggcaaaggg gagcttatac cagtactttg atgataagga tgaaatgtat
     8221 gctttcctga ttgcagaagc ttgtagcaga aggttaccat tttttaatgc ccttgatggc
     8281 aaacccagag aagattattc cttcaattac cttattattt cattaaaatt tgatgttacc
     8341 tatccttaca tcgcttcatt attctataat gggttt
//
LOCUS       DASWHX010000006        14794 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_1112238, whole genome shotgun sequence.
ACCESSION   DASWHX010000006 DASWHX010000000
VERSION     DASWHX010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 14794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14794
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1112238"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(65..1036)
                     /locus_tag="VGA21_00220"
     CDS             complement(65..1036)
                     /locus_tag="VGA21_00220"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013455126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX9648954.1"
                     /translation="MRFYSIIIPVYNRPIEIDELLTSLVLQTYKKFEVIVIDDGSTLK
                     ADKVIAKFQDQLDIHYYWKENSGQGFARNYGYENAKGDYFLVFDSDCIIPQDYLQKVE
                     DSLNQNYLDAFGGPDNADTSFTWLQRAINFSMTSKVTTGGIRGDKSSASSFTPRSFNM
                     GITAEVFNKTGGYILPRMGEDIEFSIRIRKNNFKVGLIPEAFVFHKRRTNFLQFFRQL
                     HFFGRARINVYRFHPESLKITHFFPAIFVFGIITIIFALFVAPVISIVLVYSYAFYFI
                     LIFLLSLAKTLNPVIALLSIPATFIQLTAYGIGFIIEGLRYLFGRRK"
     gene            complement(1036..2007)
                     /locus_tag="VGA21_00225"
     CDS             complement(1036..2007)
                     /locus_tag="VGA21_00225"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018478341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX9648955.1"
                     /translation="MKPGKNLSIVIPLLNEEESLAELCERIVGVCKERSFTFEIIFVD
                     DGSTDSSWKVISSLASQFPEVKGIRLSKNFGKSAALDCGFRAALGEVVITLDADLQDS
                     PDEIPALADMIRNENFDMVSGWKKKRHDPLSKTIPSKFFNGVTALFSGIKLHDFNCGL
                     KAYDYKLVRSLHLYGEMHRYIPLLAKWNGFTRIGEKIVEHRPRKFGKTKFGIERFMYG
                     FLDLLSVTFVTRFANRPMHLFGTLGTLSFLVGLGLVIKIIWEKISAIYQHLPVKREVV
                     DQPIFFIALAAIIIGVQLFLAGFLGELIIRQQKSKGDSVISEKIGME"
     gene            complement(2011..2532)
                     /locus_tag="VGA21_00230"
     CDS             complement(2011..2532)
                     /locus_tag="VGA21_00230"
                     /inference="COORDINATES: protein motif:HMM:NF025227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HEX9648956.1"
                     /translation="MEEKTTIGKVAMKYGLILGLLSIVYFLVLSFADVLGKSNLWNYL
                     GIIFTIAVIYLAHKTFKEEGDGFMSYGQGLGIGTLTSLYSAILSNIFTFVYLSYVDDT
                     MIQNIREKSISDMEARGMSQGQIDQAMGFSETFMSPGFLMIMGILGGVLIGFIVSLLV
                     SAITKNSNPQMSE"
     gene            complement(2534..3067)
                     /locus_tag="VGA21_00235"
     CDS             complement(2534..3067)
                     /locus_tag="VGA21_00235"
                     /inference="COORDINATES: protein motif:HMM:NF025227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HEX9648957.1"
                     /translation="MKFNPDLFKVPLKFGAFGGLLLSILLLILFFEGKHPLLIPIIID
                     FRLLAIPIFVFLAAKEFRDYKNGRLLQYWQGMFLGFLCYSAMGITTGIFILLFSAINS
                     EFLSEFIRISMQQLVENKAEFIKSIGAESYEANLAKLPFTTALDLSIDYLLKTIVLGL
                     LFTIIISVFLKRQPKKE"
     gene            complement(3067..4326)
                     /locus_tag="VGA21_00240"
     CDS             complement(3067..4326)
                     /locus_tag="VGA21_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012931063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEX9648958.1"
                     /translation="MKILLKDARILDPGSSFHKKKKNILIRDGIITKIGGKTYQSDEI
                     IESNNLIVSPGWFDLRSNFNDPGFEQKEDLSTGCEAAASGGFTGVAVLPNTNPPVHSK
                     NEITYLREGNNKRVTQLYPIAAVSKNLTGEELTEMLDLHNSGAIAFSDGDQPIWHTDI
                     LLKALQYLQKVNGLVINKPEDPRLNLFGNMHEGIVSTELGLKGMPSLSEEIIISRDLD
                     LLEYAGGRIHFSTISTSKSVELIRKAKRKKMHVTCDVAIINLIFDDTSVGDFDTNFKL
                     NPPLRTKNDIKALLRGIEDGTIDAIVSAHSPQDSESKNLEFGLADFGATGLQTMLHAL
                     GIITEEIPLELLIEKITQGPRTVLGLKNPRIEEKTPAELTVFDPSLEWVYNELNNKSK
                     SINSPFWNQKLRGKVLATFNNKKVYRA"
     gene            complement(4346..6358)
                     /locus_tag="VGA21_00245"
     CDS             complement(4346..6358)
                     /locus_tag="VGA21_00245"
                     /inference="COORDINATES: protein motif:HMM:TIGR02226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA domain-containing protein"
                     /protein_id="HEX9648959.1"
                     /translation="MSFLFPQFLFGLLALAIPVIIHLFNFRKTKRVFFSTTRFLQHVK
                     EASSSKLKLKHLLILCSRLLFLFFLVMAFSQPIIPAEDKDIVQNHVTIYLDNSMSMSN
                     EIDDGITAFEEGISYVQQIVEMYPPSTQYLLLTNEFAAFSSILKSKSEVLELLTEIKL
                     SGVSRSTGEISSRLRNSTLLFNNDVREIYWVSDFQKSTAGNFNILTEDSLGRYEFIPL
                     TFTSINNVFVDSVYLENPFLLSRGSNTLKVKLRNSGTGDVNDLLLKLFFNDSQIATAT
                     VDLKANAGEETHFDLGFQLENSFNTGRISFEEYPVTFDNDFFFTINRNDRIRILEIKE
                     TSAVTSVERVYGNSEMFDFKSFNTNNLDYSQIGISDLVVINGLQTIDLALSAALLDFS
                     AHNGNTLLIPSATPNIESYASLFARIGIRRVDSALMEPILPPDLRNPFFVSIFENENE
                     NFSMPEARPVITWIRDRDALLKFRSGDPFLARYSGSGYFYLMASPLSENYNTIDRHAI
                     FVPVMYKIAASGKRSSNPLFYSLNRTSVTLSLDSLSSDRVAKLTKEDLELIPFQVVRG
                     NELIMELPKHSLAAGFYQVVYDGKLKTQIAFNSTGTESILAQYDTEELNSISEEIPNM
                     RIFETENAQSFSAEIKERNLGVPLWKYALILALIFLMVEILLIRFL"
     gene            6501..6974
                     /locus_tag="VGA21_00250"
     CDS             6501..6974
                     /locus_tag="VGA21_00250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9648960.1"
                     /translation="MKTYSQKTNDFFPRQIIWLGWAFIAGSAAIAYSNWVIGLLFGSL
                     SLLLISGREGIIINFQRKRIKYYWGILGLKFGTYEDLPKFGRVTVSPQINLIKGRKEA
                     SRSKFEVRLWFREFNDYVLAFTGNHPTSSEKARILSKNLRIQFVDVSKSNGKAHK"
     gene            complement(7090..8460)
                     /locus_tag="VGA21_00255"
     CDS             complement(7090..8460)
                     /locus_tag="VGA21_00255"
                     /EC_number="4.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009582926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004122 - cystathionine beta-synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-synthase"
                     /protein_id="HEX9648961.1"
                     /translation="MYYNSIIEAIGNTPLIKLNKLSKGIKGTLLAKVEYFNPGHSMKD
                     RMALKMIDDAEKNGLLKPGGTIIEGTSGNTGMGLALIAIARGYKCIFTLSDKQSQEKI
                     DILRAMGAEVIVCPTNVAPDDPRSYYSVARKLNKEIPNSFYANQYDNLSNLQAHYETT
                     GPEIWEQTEGKITHLAAGVGTGGSICGTAKYLKEQNSSIISIGIDTYGSVFKKYKETG
                     VFDVKEIYPYLTEGIGEDILPKNVDFSLIDHFVKVTDKEGAVMTRKLAREEGLFVGWS
                     CGSAVHGALEYAKENLKDSDMMVIILPDHGTRYLAKIFNDNWMRDHGFLEARDYATAK
                     DIINRRNGKQVLKTIPVTMKTGEAILLMNKEGISQLPVEDNGNFVGSIIDSRILHKII
                     ENPDLRNEPVKEIMDEPLIFVGMDNTVDVLSSLIDHSQQALLVRDENNEVHIITQHDL
                     LMAMAY"
     gene            8558..8986
                     /locus_tag="VGA21_00260"
     CDS             8558..8986
                     /locus_tag="VGA21_00260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9648962.1"
                     /translation="MDKTIGIILIIGFIGFQACKKDDEVLTNHQLLAGTTADGKSWVI
                     ASQSLNGDPVTIGSCIADDVTSFYLSLIYIFDEGSQKCSADDPQTITGGWVFEENDTR
                     LLISNGPDITDFEIVSLTADELILKAQFLDLETVITFNPL"
     gene            complement(8989..9915)
                     /locus_tag="VGA21_00265"
     CDS             complement(8989..9915)
                     /locus_tag="VGA21_00265"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009185518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEX9648963.1"
                     /translation="MDKLSVKHLLGIKSLTSNDIELIFKTADNFKEIINRPIKKVPSL
                     RDVTIANIFFENSTRTRLSFELAEKRLSADVINFSSSSSSVKKGETLLDTVNNILAMK
                     VEMVVMRHYSPGAPHFLARHIKANIINAGDGTHEHPTQALLDAYSIREKHKTVAGKKI
                     AIIGDILHSRVAISNIFALQKLGAEVMVCGPNTLLPKHIASLGVKVELDVRKALEWCD
                     VANVLRIQLERQQIKYFPSLREYSLYFGINKKMLDSLNKKITIMHPGPINRGVELSSD
                     VADSDHSIILNQVENGVAIRMAVLYLLAGSPQ"
     gene            complement(9922..10467)
                     /gene="pyrR"
                     /locus_tag="VGA21_00270"
     CDS             complement(9922..10467)
                     /gene="pyrR"
                     /locus_tag="VGA21_00270"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013452952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEX9648964.1"
                     /translation="MHKRLILDSNQLQITIHRLCQQLIENHNNFDDSAIMGLQPRGIY
                     LAEKIKENLEKSLKKSVLLGYLDTTFYRDDFRRRETPVTAKSTQVDFIIERKNVIIVD
                     DVLFTGRSVRAAMDAMMAFGRPRKVELLVLVNRKYSRELPIEPDYVGKNVNTIESQKV
                     LVEWVKSGSKSDNVWLITKED"
     gene            complement(10505..12028)
                     /locus_tag="VGA21_00275"
     CDS             complement(10505..12028)
                     /locus_tag="VGA21_00275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9648965.1"
                     /translation="MYIRVARAILFFVFSISFIFKSFSQIEQPTRFEIGMGTFDRGFE
                     IIALKDRGIVLYRQTNDRSGDGRTWQVIYLDNNLEMIWDKEYVLPFELELVGYAGNSR
                     YFYLLFSNKKTNNKQFELYEFDFEGNSPSNYTITNLFPMYLSHFEVSDNAAILGGYLN
                     YKPVVIHFGFSNQRPIVLPTIYNRNTEIIQVKVNNDNTITVLISERTQDNRITIAIKH
                     FDKDGALLLSNALEPSAGNNFIFGRVAGDDSGAVLVAGTYSTTKSAYSRGIFISRLGQ
                     DGSENSNYYNYADLKNFFNYMKASREKRVRERIARKKIKGKKLRFNYRLLVHEIIDTE
                     DGFIMLGEAFYPKYSSRGSMGYEAVLNPMTGNYLGMYLEGYKYTHAVVIEFDEKGRIK
                     WDNSFEITDVLTSNLNQFVNATVEGDNIVLLYLFENIIRSKIIQGDQVLEGKSYEDLR
                     LKYENDQVRNSDVEFGGIEKWSDDTFVAYGVQRIRNMKDSGVDLNRRVFFVNKLIYK"
     gene            complement(12029..13609)
                     /locus_tag="VGA21_00280"
     CDS             complement(12029..13609)
                     /locus_tag="VGA21_00280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9648966.1"
                     /translation="MKPKPLSLLKIFTYLFFSLLAVPADSLLAQISQPMRFEIQKKAN
                     ESYWNVVSAHEFGIAMFRENRRSDLGNKYWEVLMLDTDLNLKWEKEFPADRRNTFRGF
                     DYSNNQLFLLFQEGSQDRGKYEIMRIQLANGNIKNFSVEHQVPLELEDFITVGESAVF
                     GGYANGLPTVLMFNFAEDKFKVIPGLYRKNAYLLDLSADEVNNTFSIVINERYSQQEN
                     NLTIKTFNSSGDILTDKQVSLDENTNILSAKTTTFDWGEQVITGTFGERNSDFAKGVY
                     FVRIQEQQESALLQYNFTELENYFNYLSGKQKERMDTRIEQLKAKGKELNVKSRIVLH
                     ELIPEGSEYIMLGEVYDVNFGTMNNSPYGTPYYMNNFNSGYRGYRVALPNAQSIDRPA
                     TFEYKQAIIIGFSQTGEIKWDNSLVLNELYVAYLNQMVHINTINNRSVLIYKDNSELI
                     AIIFEKGGESSGTIEEIVKLKFEGDELRSEAKQFSGLSYWYEDNFFTWGYQRIRNKLD
                     ERAPKSRNVFYINKIKIR"
     gene            complement(13643..14047)
                     /locus_tag="VGA21_00285"
     CDS             complement(13643..14047)
                     /locus_tag="VGA21_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009281999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HEX9648967.1"
                     /translation="MNYWLVKSEPGTYSWDNLEKDGKTCWDGVRNFKARNNLKAMKSG
                     DHALFYHSGEQKAVVGVCKVIKEGYPDPSAKEGPWIAVDLSPVKKLKNPVTLAEVKAD
                     SQLTNMILARQPRLSVQPVTKEEYNLIVTKSK"
     gene            14391..>14794
                     /locus_tag="VGA21_00290"
     CDS             14391..>14794
                     /locus_tag="VGA21_00290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9648968.1"
                     /translation="MNLEAEVFCLIDMENKDAINENPYISEIFLKEETSVSKLIRSNS
                     FDLVIDLEIKFTTWLFSILSGIKILKPKSQKFAEWYMVALRIDQLPDKHYADQLLEVL
                     DSLSSNRDVLGLDYFIPEKDEIERSWLPDIFQ"
BASE COUNT         4386 a         3000 c         2704 g         4704 t
ORIGIN      
        1 gataaatatg catcctatct gtctgatgag gatattaagc gatatgcttt gcagagaaat
       61 gaaattattt tcttctgcca aaaagatagc ggagaccttc gataataaac ccaattccgt
      121 aagcggttaa ttgaataaat gtggcaggta tgcttagcag ggcaataacc ggatttaaag
      181 ttttagcgag gcttagtaaa aaaatcaaaa taaagtaaaa agcgtaagaa tatactaaaa
      241 caattgaaat caccggcgct acaaacagcg caaatattat tgtaatgatt ccaaacacaa
      301 atatggctgg gaaaaaatgg gttattttta acgactccgg atgaaatctg taaacattaa
      361 tccgggcacg gccgaagaaa tggagctgcc tgaagaattg caaaaagttt gttcttctct
      421 tatgaaatac gaaagcttcg ggtattaatc ccaccttaaa attatttttc ctgattctga
      481 tactaaactc aatatcctcc cccatcctgg gaagaatgta tcctccggtt ttattgaata
      541 cttccgcggt gatgcccata ttaaaactcc tgggagtaaa acttgaagcg gaacttttgt
      601 ctcctcttat gccaccggtt gttactttgg acgtcatgga gaaattaatt gcccgctgca
      661 accaggtgaa cgaagtgtca gcattgtcag gtcctccgaa agcatccagg taattttggt
      721 tcagggagtc ttccaccttt tgaagataat cctgtggaat aatgcaatct gaatcaaaaa
      781 ctaggaagta atctcctttt gcattttcat agccgtaatt cctggcaaac ccttgtccgc
      841 tattttcttt ccagtagtag tgaatatcaa gctgatcctg aaatttagca atcaccttat
      901 ctgcctttag ggtggagcca tcatcaatta caataacttc aaatttttta taagtctgca
      961 gaacaagact agtgagcaat tcatcaattt caatcggtct gttataaacc ggaattatta
     1021 ttgaatagaa tcgcattatt ccatgccaat tttttcagaa attactgaat cacctttgga
     1081 tttttgctga cggattatca attcacccag aaaaccggcg aggaaaagtt gaaccccaat
     1141 aataatggca gccagtgcaa tgaagaatat tggctggtcc accacctctc ttttcaccgg
     1201 gagatgttga taaattgcag agattttctc ccagataatt ttaattacaa gtccaaggcc
     1261 aaccagaaaa gaaagtgttc ccagagttcc aaacaaatgc atgggacgat tggcaaacct
     1321 tgttacaaag gttaccgata agagatcaag gaatccgtac ataaatcttt ctataccaaa
     1381 tttagttttt ccaaattttc tgggccgatg ctccacaatt ttttctccaa ttctcgtaaa
     1441 tccattccat tttgccaaca gggggatata tctgtgcatc tccccataaa ggtgcaaact
     1501 acgaactaat ttataatcat aggctttcag gccacagtta aagtcatgga gttttattcc
     1561 tgaaaataag gccgtaaccc cattaaagaa ttttgaaggg atagttttgg aaagcgggtc
     1621 atggcgtttc tttttccagc ctgaaaccat atcaaagttt tcattcctga tcatatcggc
     1681 cagagccggt atttcatcag gggaatcctg caggtctgca tccagggtaa taaccacttc
     1741 acccagggcc gctctgaatc cacaatctaa agcagccgat ttgccgaagt ttttacttaa
     1801 tcgaattcct tttacttcag gaaactgact tgctaaagaa gagataactt tccaggagct
     1861 atccgtactc ccatcatcga caaagattat ttcaaaggta aaactacgtt ccttgcatac
     1921 tcccacgatc cgctcacata actccgcaag ggattcttct tcattgagga gaggaattac
     1981 tatggaaagg tttttaccgg gcttcaagta ttactcagac atttgcggat ttgaattctt
     2041 ggtgatggct gaaacaagca gagagactat aaaaccaatt aaaacccctc ctaaaatacc
     2101 cataatcatt aaaaagcccg ggctcataaa ggtctctgaa aatcccattg cctgatcaat
     2161 ctgtccctgc gacattcccc ttgcctccat atccgaaata gatttctccc tgatattttg
     2221 aatcattgta tcatcaacat agctgagata tacaaaagtg aatatgttgc tcaggattgc
     2281 tgaatataaa ctagttaggg ttccaattcc taacccctgg ccatacgaca tgaaaccatc
     2341 cccttcttct ttaaaagttt tatgggcaag atagattacg gcaattgtaa atatgattcc
     2401 caggtagttc cataaattgc ttttgcccaa aacatcggca aagctgagta cgagaaaata
     2461 aacaatgcta agtaagccta aaatgaggcc gtatttcatg gctacttttc cgatggtggt
     2521 tttttcttcc attttattct tttttaggtt gacgttttaa aaatacagaa attattatag
     2581 tgaaaagtaa ccccagaaca atagttttca gcaaataatc tatggaaagg tcaagggcgg
     2641 ttgtaaaagg caatttggca agattagcct cataagattc agcacctatt gacttaatga
     2701 attcggcttt attttccact aattgttgca ttgatattct tataaattca gacaaaaatt
     2761 cgctattaat agcgctgaac aatagtatga aaattccagt agtaatgccc attgccgagt
     2821 agcaaaggaa gccgaggaac ataccctgcc agtattgtaa aagccggcca tttttataat
     2881 ctctgaattc tttggcagcc aaaaaaacaa aaattggaat tgctaataat ctaaagtcaa
     2941 taataattgg aatgagcaat gggtgttttc cttcaaagaa aagaatcaac aggagaatgg
     3001 ataaaagcag acctccaaaa gctccaaatt tcaaagggac tttaaaaaga tcgggattaa
     3061 atttcatcag gccctgtaaa cctttttatt attgaaagtg gcaagaactt ttccgcgtaa
     3121 tttctggttc cagaatggcg aattaattga ttttgattta ttatttaatt cgttgtaaac
     3181 ccattccaga gaaggatcaa atactgtcaa ttcagccgga gttttttctt caatcctggg
     3241 attttttaat cctaaaactg ttcgcggacc ctgggtaatt ttttcaatta gaagttcgag
     3301 cggaatttct tcggtaataa tacccaacgc atggagcata gtttgaaggc cggtggcacc
     3361 aaaatctgcc aggccgaatt ccaggttctt gctttcagaa tcttgtggag aatgggcgct
     3421 aacaatggca tctatagtcc catcttcaat tccacgtaac aatgctttga tgtcattctt
     3481 tgttctgagc ggaggattga gtttaaaatt tgtgtcaaaa tcacccacac tggtatcatc
     3541 aaagattaga tttatgatag ccacatcgca ggtcacgtgc attttttttc ttttggcttt
     3601 tctgatcaat tcaactgact ttgaggttga aatggttgaa aagtgaattc tcccaccggc
     3661 atattctaat aaatccaggt ctcgagatat tatgatttct tccgacagcg aaggcattcc
     3721 ttttaaaccc agttctgtac taacaattcc ctcgtgcatg ttaccaaaca aatttagtct
     3781 tgggtcttca ggtttattaa ttaccaatcc atttaccttt tgcaagtatt ggagagcttt
     3841 gagaagtata tctgtgtgcc aaatcggctg atcaccgtct gaaaatgcaa tcgcgccgga
     3901 attgtggagg tccagcattt cggttagctc ctcaccagtc aggtttttcg ataccgcagc
     3961 aattggatac aattgagtaa cccttttgtt gttaccttct cgcaagtatg tgatttcatt
     4021 cttggaatga accggggggt ttgtattggg cagcacagcc acaccggtga agccgcctga
     4081 tgccgccgct tcacatccgg tacttaaatc ttctttttgt tcgaaaccgg ggtcattaaa
     4141 atttgatcgt aaatcaaacc aaccggggct cacaatcagg ttgttgctct ctatgatttc
     4201 gtcggactgg taggtcttcc cgccaatttt agtgatgatc ccgtccctga ttagaatatt
     4261 tttcttcttt ttgtgaaagg aagagccagg gtcaagaatt ctggcgtctt ttagaagtat
     4321 tttcatttac cttttatagg aatctttata ggaatctgat tagcagaatt tcaaccatta
     4381 aaaatattag cgctaatata agtgcatatt tccataaagg cacaccaaga ttacgttctt
     4441 ttatttctgc gctaaaggat tgggcgtttt ctgtttcgaa gatgcgcata ttcggtattt
     4501 cctcggaaat ggaattgagt tcttcggtat catattgggc aagaatcgat tcagttccgg
     4561 tactgttaaa agctatttga gttttcaatt ttccgtcata aactacctgg tagaaaccgg
     4621 cggccaaaga atgtttggga agttccataa tcaattcatt cccgcgcacc acctgaaatg
     4681 gaataagttc aaggtcttct ttggttaatt ttgctacacg gtcagaagat aaactatcca
     4741 gggatagtgt aacggaagtc ctgttcaggg agtagaaaag gggattggat gaccgtttcc
     4801 ctgaggctgc aattttatac atcaccggaa caaaaattgc atgcctgtca attgtattat
     4861 aattttcaga taacggtgaa gccatcaagt agaagtaccc gctgccacta tatctggcca
     4921 aaaaaggatc accgctcctg aacttgagca gcgcatcgcg gtctcttatc catgtgatta
     4981 caggtcgcgc ttccggcata ctgaagtttt cattctcatt ttcaaatatg cttacgaaaa
     5041 aaggatttct taaatccggc ggcaaaattg gttccattaa tgcagagtcg actctcctga
     5101 ttccaatcct ggcaaacaaa cttgcataac tttcaatatt tggagttgcc gatggaatta
     5161 atagagtatt cccgttatgg gctgaaaaat ccaataatgc agcacttaac gctaagtcaa
     5221 tagtctgaag gccattgata accacgaggt cggagattcc aatctgactg taatcgagat
     5281 tattcgtgtt aaaggatttg aagtcaaaca tttcgctatt tccataaaca cgttctacag
     5341 aagtcacggc agaggtttct ttaatttcca aaattcttat tctgtcattt ctgtttatgg
     5401 taaagaaaaa atcattgtcg aaggtcacag ggtattcttc aaacgaaatt ctgcctgtat
     5461 tgaaagaatt ttcaagttga aagccaaggt caaaatgagt ttcttcacct gcgttagcct
     5521 ttaggtctac agtagctgtt gctatctggg agtcattaaa aaaaagtttg aggagcaaat
     5581 catttacatc tcctgttccg gaattgcgca gtttaacttt aagtgtattg cttcctcttg
     5641 aaagaaggaa aggattttcc agatagactg aatcgacaaa aacattattg atactggtga
     5701 aagtgagcgg gatgaactca tacctgccaa gagaatcttc agtaagaata ttgaaattac
     5761 cggcggtaga tttttgaaaa tcactaaccc agtaaatttc tcgaacatca ttattaaata
     5821 agagagtgct gtttctgagc cgggaactaa tttcacctgt tgaccttgaa actcctgata
     5881 gtttaatttc ggttaagagt tccagaacct cgcttttact tttcagaata cttgagaatg
     5941 cagcaaattc attggttaac aacaggtatt gtgtgctggg cggatacatt tcaacaatct
     6001 gctgaacata agaaattcct tcctcaaacg cggttattcc atcgtcaatt tcattagaca
     6061 tactcattga attatccagg tatattgtta catgattctg aacgatatcc ttgtcttcag
     6121 caggtatgat cggctgacta aatgccatca cgagaaaaaa gagaaaaagc aacctggaac
     6181 agagaatcaa taagtgttta agcttcagtt tggaactgga tgcttccttt acatgttgaa
     6241 gaaacctcgt agttgaaaag aaaactctct tggttttacg aaagttaaac aggtgaataa
     6301 ttaccggtat tgccagtgct aataagccaa ataaaaactg agggaagaga aaactcacga
     6361 ttcgatctga tagattactt cttaattaac ggaaattaga taccaacagg tgattccggg
     6421 caaaatttgg tccccaattc agcccttata attctaaatt ctctgcaatt ttataaatta
     6481 agcatcaata acttttcatc ttgaaaacat attctcaaaa aaccaatgat tttttccccc
     6541 gtcaaattat ctggctgggc tgggctttca ttgcaggctc ggcagcgatt gcttactcaa
     6601 attgggttat tggcttactt ttcggttctt tgagtttatt gttgatcagt ggccgggaag
     6661 gtattatcat taattttcaa agaaaaagga taaaatatta ttggggtatt ttgggtctca
     6721 aatttggcac ttatgaagat ttgccgaagt ttggcagggt aacggtatct cctcaaatta
     6781 acctgattaa agggagaaaa gaggcctcaa ggtccaaatt cgaagtgcgg ctttggttca
     6841 gggaatttaa tgattatgtg ctcgctttta cgggcaatca ccctacaagt tcagaaaaag
     6901 cccgaattct ttctaaaaat ctaagaattc agtttgttga tgtttcgaaa tcaaacggaa
     6961 aagctcataa ataagaaagg gctgcttttt gcaagcagcc ctaactccaa acttacctaa
     7021 ttaaatttat caaccacctt taggaattaa acaaaatcgg tgccaaaagg tttcattttt
     7081 gagcatattt tagtaggcca tagccattag gagatcatgt tgggtaatta tgtggacctc
     7141 attattttca tctctaacaa gtaaagcttg ctggctatga tctattaatg atgacagtac
     7201 atccacggtg ttatccattc caacaaagat caacggttca tccattattt cttttacagg
     7261 ttcatttctg aggtcaggat tctcaataat cttgtgcaga atccgtgaat cgatgatgct
     7321 tcctacaaaa ttcccgttgt cttctacagg aagttgagaa ataccttcct tgttcattag
     7381 aaggattgcc tctcccgttt tcatagttac aggaatggtt ttcaaaacct gttttccgtt
     7441 tctcctgtta atgatatcct tcgcggtggc ataatctctg gcttccagaa atccatggtc
     7501 acgcatccag ttgtcattaa aaatttttgc aaggtagcgg gtaccatggt ccggcagaat
     7561 tatcaccatc atatcactat ccttcaaatt ttctttggca tattccaaag cgccatgcac
     7621 agcagatccg caggaccagc ccacaaaaag cccttcttca cgagccagtt ttctggtcat
     7681 caccgcgcct tctttatccg ttacctttac gaaatggtca atcaggctaa aatcaacgtt
     7741 tttgggcaga atatcctctc caattccttc agtcagatat ggataaatct cttttacatc
     7801 aaaaactccg gtttccttat atttcttaaa gacacttccg taagtatcaa tccctatgga
     7861 tattatagat gaattttgct ccttgagata ttttgcagtt ccacaaattg aacctcctgt
     7921 tccgacgcca gcagccaaat gggtaatttt accttctgtt tgttcccaaa tttccggacc
     7981 tgtagtttcg taatgtgctt gcaggttaga caaattatca tactgattgg cataaaaaga
     8041 attcggaatt tccttattta gttttcgtgc tacagaatag taggagcgcg gatcatcagg
     8101 agcaacattg gttgggcata caattacttc agctcccatt gctcgcagga tatcgatttt
     8161 ctcctgggat tgtttatcgc tcagggtgaa aatacatttg tatcccctgg caatggcaat
     8221 taaggcaagt cccataccgg tattaccgga agttccttct attatagtcc ccccgggttt
     8281 gagcaaacca tttttctccg catcatcaat cattttaaga gccattctgt ctttcatgga
     8341 gtggccagga ttaaaatatt cgactttagc caatagtgtt cctttaatgc cctttgaaag
     8401 cttattcagt ttgattaagg gagtatttcc tattgcctca attattgaat tataatacat
     8461 aacagaattc gttgtatgac gcaaagttac aaattttaca ctgctattaa gggaagggtt
     8521 ttgtggtaat atttattgaa gtatttttat tgtcgatatg gataaaacga taggcataat
     8581 tctcatcata ggtttcattg gtttccaggc gtgcaaaaaa gatgacgagg tacttaccaa
     8641 ccatcaatta cttgcaggaa cgacagcgga tggaaaatca tgggttattg ccagccaaag
     8701 cttaaatgga gatcctgtta ccattggcag ttgcatagca gacgatgtca cctcttttta
     8761 tctttcgtta atttatattt ttgatgaggg tagtcagaaa tgcagcgcag acgacccgca
     8821 aacaattacc ggagggtggg tttttgaaga aaatgatacc cgcctgctca tttcaaatgg
     8881 tccggatatt acagattttg aaatcgtttc attgaccgcg gatgagttaa tattaaaggc
     8941 tcaatttctt gatctggaaa ctgtaatcac cttcaatcct ttgtaggatt attgcggaga
     9001 acctgcaagc agatagagaa ccgccattcg aatagcaaca ccattttcta cctggttcaa
     9061 aattatggaa tggtctgaat cagcgacatc acttgatagt tcaactcccc tattgatggg
     9121 acctggatgc atgatggtaa tctttttatt tagcgaatcc agcattttct tattgattcc
     9181 aaagtacaaa gaatattctc tgaggctggg gaaatatttt atttgctgtc gctccagttg
     9241 gattctaagc acattggcca catcgcacca ttcaagagct tttctaacat ctaactccac
     9301 tttcacgccc agggatgcaa tatgcttggg caacagagta ttaggcccgc aaaccattac
     9361 ctccgctccc agtttttgca atgcaaaaat gtttgagatt gccacgcgtg aatgaagtat
     9421 atctccaatt atggcaatct ttttcccagc cacagttttg tgtttttcgc gtatagaata
     9481 ggcatcaagc aaggcctgtg tcggatgttc gtgagttcca tcaccggcat taataatatt
     9541 tgcttttata tgcctggcca ggaaatgagg agcacccggg ctgtagtgcc gcataactac
     9601 catttcaact ttcattgcca gaatattatt cactgtatcc aggagagtct cccctttttt
     9661 gacggagctt gaagatgacg agaaatttat gacatctgct gaaagtcttt tttcagcaag
     9721 ctcgaatgaa agccgggtgc gggtagagtt ttcgaagaag atattggcaa ttgttacatc
     9781 cctgagggat ggcactttct taataggccg gttaataatt tctttgaagt tgtcggcggt
     9841 tttgaaaata agttcaatat cattggaggt aaggcttttt attccaagca agtgctttac
     9901 gctaagctta tccataatga tctaatcttc ttttgttatt aaccaaacgt tatccgattt
     9961 gctgccagac ttaacccatt caaccagaac tttttgtgat tcaattgtat taacattttt
    10021 gccaacataa tctggttcta tgggtaattc cctggagtat tttctattta cgagtacaag
    10081 taattctact ttacgcggac gtccaaatgc catcatggca tccatggcag cccggactga
    10141 acggccggtg aaaaggacat catccacaat aattacattc ttgcgctcaa tgataaagtc
    10201 aacctgggtt gattttgctg tcaccggggt ttccctgcgt ctaaaatcgt cccggtagaa
    10261 agtagtgtct aaataaccaa gcaaaacaga ttttttcaga gatttttcca ggttttcctt
    10321 aattttctcg gccaggtata taccacgggg ctgcaatccc ataatagcgg agtcatcaaa
    10381 attattatgg ttttcgatta actgctggca caaccggtgg atggttattt gtaattggtt
    10441 ggaatccagg ataagccttt tatgcattgc tgcaaatata ggaaagaagc ttaattttca
    10501 gaagctattt atagatgagc ttatttacaa aaaatactct tcgattgagg tctacacccg
    10561 aatccttcat attcctgatt ctttgaacgc cataggcaac gaaagtatca tcactccatt
    10621 tttctatccc tccaaattcc acgtccgaat tccgcacctg gtcattttca tactttaatc
    10681 taagatcttc atatgattta ccttccaaaa cctgatcccc ctgaataatt tttgacctga
    10741 taatattctc aaaaaggtat agtagaacaa tattgtctcc ttcaaccgtt gcatttacaa
    10801 actgattcag gtttgaagtt aatacatcgg ttatctcaaa actgttatcc cattttattc
    10861 tgcctttttc atcaaattct atcacaactg cgtgcgtgta tttataacct tcaaggtaca
    10921 ttcccagata attaccggtc atcggattga gcactgcttc gtaccccata gatccccggc
    10981 ttgagtattt gggataaaac gcttcgccaa gcattataaa accatcttca gtatcaatta
    11041 tttcatgcac cagaagtctg tagttgaaac gcaatttttt tcctttaatt tttttcctgg
    11101 caattctttc tctgactcgt ttctcccggc tggccttcat atagttgaag aaatttttca
    11161 aatcagcata attataatag ttcgagtttt cgctcccatc ttgcccaagc ctgctaataa
    11221 aaattccccg gctatacgct gatttagtag tagaataagt tcctgccacc agtactgcac
    11281 cggagtcgtc tccagctact cttccaaaaa taaaattgtt acccgcactt ggctccaatg
    11341 catttgataa caacaaggcg ccatccttgt cgaagtgttt aatggcaatt gttatcctgt
    11401 tgtcctgtgt tctttcactg attaacactg taattgtatt atcattatta acctttacct
    11461 ggattatctc tgtattccta ttatatattg taggtaaaac gataggtctt tggtttgaaa
    11521 atccaaaatg aatcacaacc ggcttatagt tcaggtaccc tccaagaata gccgcattgt
    11581 cactcacctc aaaatgagac aaatacattg ggaacaggtt ggttatcgta taattacttg
    11641 gtgaatttcc ttcaaaatca aattcataca attcaaattg cttgttgttt gtttttttat
    11701 tagagaataa taaatagaaa tacctggagt tgcccgcata acccactaat tccagttcga
    11761 atggcaacac atactcttta tcccagatca tttcaagatt attatccaga tatattacct
    11821 gccacgttct accatctcca ctgcgatcgt ttgtttgtct gtaaagaact attcccctgt
    11881 cttttaatgc tattatttca aagcccctgt caaaagttcc cattccaatc tcaaatcttg
    11941 taggctgttc tatttgagaa aagcttttga aaataaatga aattgaaaat acaaaaaata
    12001 aaattgccct ggcaactcta atatacatct acctaatctt tattttattt atataaaata
    12061 cgttcctgct cttgggagca cgctcatcca atttattcct gattctctga taaccccatg
    12121 taaaaaagtt gtcttcatac caatatgaca gaccgctaaa ttgtttagct tctgatctta
    12181 attcatcacc ttcaaatttc aactttacga tttcttcaat tgtacctgaa ctttctccac
    12241 ccttttcaaa aattatcgca attaattcac tattgtcctt ataaataagc acagaccggt
    12301 tattgatggt gtttatatgt accatttgat ttaaataagc cacatacaat tcattcagaa
    12361 caagactgtt gtcccactta atttccccgg tctgactgaa tccgatgatt atggcttgtt
    12421 tgtattcaaa agttgccgga cggtcaatgg actgtgcatt gggtaatgct accctatagc
    12481 cgcgataacc actgttaaaa ttattcatat aataaggtgt gccatacgga gagttgttca
    12541 ttgttccaaa attcacatca taaacctcac caagcattat gtattcgctt ccttcaggta
    12601 ttaactcatg taaaacaatt cgtgatttca cattcaattc cttcccttta gcctttaatt
    12661 gttcgattcg agtatccatt ctttcctttt gtttgcccga caggtagtta aaataatttt
    12721 ctaactcagt aaaattatat tgaaggagag cggattcctg ctgttcctga attcttacaa
    12781 aatatacgcc tttcgcaaaa tctgaatttc gctcaccaaa agttccggta ataacctgct
    12841 cgccccaatc aaaagtggtg gtctttgctg acaagatatt tgtattttca tcgaggctta
    12901 cctgcttgtc ggtaagaata tcaccgctac tattaaaagt cttaattgta agattatttt
    12961 cttgttgtga atacctttca tttataacta tggagaacgt attatttact tcatccgcgc
    13021 ttaaatcaag gagatatgca ttcttcctgt acaaaccggg aattactttg aatttgtctt
    13081 ctgcaaaatt gaacattaga actgttggca atccatttgc ataacctccg aaaacagcac
    13141 tttctcctac cgtgataaaa tcttcaagct ccaacggtac ctggtgctcc acagaaaagt
    13201 ttttaatatt accgtttgct aattgaatac gcatgatttc atatttgcct ctatcctggg
    13261 aaccttcctg gaagagaagg aataattggt tgttgctgta gtcaaatcca cggaaagtat
    13321 tacgtctgtc agcgggaaac tctttctccc atttcaaatt cagatctgta tccagcatca
    13381 atacctccca gtatttatta cctaaatcac ttcttctatt ttcacgaaac attgctatgc
    13441 caaattcatg ggcggaaact acattccaat aactttcatt ggctttcttc tgtatttcaa
    13501 acctcatggg ttggctaatc tgggctaaaa gtgaatcagc aggaacagcc agcaggctaa
    13561 aaaagaggta agtaaagatt ttcaataagc ttaagggttt cggtttcatt taaaggaagc
    13621 aattattatt cccaatttac tgttacttac ttttggtaac gatcaggtta tactcctctt
    13681 ttgtaaccgg ttggactgaa agcctgggct gtcttgccag tatcatgtta gtcaactgag
    13741 aatctgcctt aacctctgcc agcgtaaccg gattctttaa tttttttacc ggtgacaaat
    13801 ccacagcaat ccatgggcct tcctttgcag aaggatccgg ataaccctct ttaatgactt
    13861 tacacacacc cacaactgcc ttttgttcac cactgtgata aaacaaagca tggtcacctg
    13921 atttcattgc ttttaaatta tttctggctt taaaatttct aactccgtcc cagcaggttt
    13981 ttccatcttt ttcaagattg tcccaggaat aagttcccgg ttccgatttt actaaccaat
    14041 agttcatttg tatgtgggtt tataaattta aatttaatca aaagtctagt aattttaagg
    14101 caatggacaa tttaggtaga gtctcaggtg gagtctttta ggtgtgccac acctttacca
    14161 acattgaaaa tcaatccatt ttcacctaaa ataaaaagtg gaagtttggc acagttgatg
    14221 ttaatttcat ttggatttaa acggagacac taccacaatt tactcgctta caacttagaa
    14281 atgtctttgc ataataataa tcagatagcg acaaaatgaa agtattgatt tgccatactt
    14341 cggattttgg taatcttatt tttgctactc ccttgatccg aaatctgaaa atgaatttag
    14401 aagcagaggt attctgcctg attgatatgg aaaataagga tgcaattaat gaaaatccat
    14461 acatttctga aatattttta aaggaagaaa caagcgtttc aaaactcatc cggagcaatt
    14521 cttttgattt agtaattgat ttggagatta agttcaccac ctggctattt tccatactct
    14581 ccggaataaa aatattgaaa ccgaaaagcc aaaaattcgc ggaatggtac atggtagcgc
    14641 taagaattga tcaattgccg gataagcatt acgcagacca gctgcttgag gttcttgaca
    14701 gcctgagtag caatagggat gtgttgggtc tggattattt tattcctgaa aaggacgaaa
    14761 ttgaaaggag ttggttaccc gacatctttc agaa
//
LOCUS       DASWHX010000007        15483 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_1112360, whole genome shotgun sequence.
ACCESSION   DASWHX010000007 DASWHX010000000
VERSION     DASWHX010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 15483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15483
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1112360"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2500
                     /gene="clpB"
                     /locus_tag="VGA21_00295"
     CDS             <1..2500
                     /gene="clpB"
                     /locus_tag="VGA21_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009582963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HEX9648969.1"
                     /translation="SSDENMISFLFKKSGVNRQTFLEGLEDIVTKYPKVSGQQPYLSN
                     DSNKVLQLANDFLKEFKDEFVAVEHLFLGIFSGNDKVAVLMKQSGFTKPVLIKSIKEL
                     RGNARVTDQNAESKYRSLERYSRNLNELAAKGKIDPVIGRDDEIRRVLQILSRRTKNN
                     PILLGEPGVGKTAIVEGLAQRIVDGDVPENLKSKIIVSLDMGLLIAGAKYKGEFEERL
                     KAVIKEVTDSNGEIIMFIDEIHTLIGAGSGGEGAMDAANLLKPALARGELHAIGATTL
                     KEYQKFIEKDKALERRFQTVMVDEPSLEDSISILRGIKDRYELHHGVRIKDDAVIAAV
                     ELSSRYITDRYLPDKAIDLVDEAASKLRLEIDSLPEELDELQRKIMQLEIEREAIRRE
                     KDKDKERTLSKAIAELGESRDHLKAKWKNEKEIISGIQHEKENIEKYRLEAERAEKAG
                     DYGRVAELRYGIIQEAEAKLELLKKQMNEIQAESPLLKEEVDNEDIADVVAKWTGIPV
                     SKMLQSDREKLLHLEDELGKRVAGQSEAIQALSDAVRRSRAGLQDPKRPIGSFIFLGT
                     TGVGKTELSKALAEYLFNDENAMVRIDMSEYQERHSVSRLVGAPPGYVGYDEGGQLTE
                     SVRRRPYSVVLLDEIEKAHPDVFNILLQVLDDGRLTDNKGRVANFKNTIIIMTTNIGS
                     HIIQESFERMNEENAEEVMEATRLQVMELLRKSVRPEFLNRVDETIMFRPLTKEDIRK
                     IVELQFGIIKKRLDEAGVKAEISDKAINYLAKAGFDPQFGARPLKRVMQKLILNELSK
                     QILEGKIAKDGIIGIDVGDNNQLEFLNLDKVELS"
     gene            complement(2552..3889)
                     /locus_tag="VGA21_00300"
     CDS             complement(2552..3889)
                     /locus_tag="VGA21_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011585657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEX9648970.1"
                     /translation="MAKGRSQGKIFVLDTSVILYESNSIMNFDEHDVAIPITVLEELD
                     QFKKGNDIINYEAREFIRLLDNLAQGHTLQNWVPLNGRSKGKFKVVMNTTNGVDAVKI
                     FDEEKADHRILNSALALQRENPNRKVILVSKDINLRLKAKSLNLPAEDYTTGKIQNVN
                     SLFSGKSELNEVPPEVINKIYEKGFCAPEEILYKSILIANNYFIIKNDKKSVLAYYNP
                     ESQLVEKVEKRMIYGIKPRNAEQTFAIHALLNPKVRLVSIIGVAGTGKTLLALSSALE
                     QKRDFKQIYLARPIVPLSNKDIGYLPGDIKSKLNPYMEPLWDNLKFIQNQYKETDKEY
                     SKITEMVNQEKLVITPLAYIRGRSLSNIFFIVDEAQNLTPHEVKTIITRAGENTKLIF
                     TGDINQIDTPYLDSQSNGLSYIIDRIKNHNLYAHITLEKGERSELANLANELM"
     gene            complement(3962..4615)
                     /locus_tag="VGA21_00305"
     CDS             complement(3962..4615)
                     /locus_tag="VGA21_00305"
                     /inference="COORDINATES: protein motif:HMM:NF016232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9648971.1"
                     /translation="MTIRELRSLVRKGENDHLEFKKKVDYPEKVIKEITAFANTQGGE
                     LLIGVDDNREISGLKYPDEHRFALENAIESFCKPVISFHVEQIPVSENRFVLRYTIDK
                     SERRPHSVRLAPGKDEYFIRVGDKSVKASREVVEIMERQKKGKDIQFYFGDSEKILMN
                     YLEENNFITIDQYALVARLKPYQAARKLILLVLANVLMVVPGEKHDVFGLNKIATEQ"
     gene            complement(4619..6136)
                     /locus_tag="VGA21_00310"
     CDS             complement(4619..6136)
                     /locus_tag="VGA21_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018618595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase"
                     /protein_id="HEX9648972.1"
                     /translation="MSKDIDYSKISTLGDLKRSGYKTKSVKLELRDNLIAKLKKKEKV
                     FDGIWGYEETVIPDLERAILSMHDINLLGLRGQAKTRLARLMIDLLDEFIPVIKDTEL
                     NDDPFNPISRQARDIIEECKDATPVSWMHRGERYTEKLATPDVSIADLIGDVDPIKAA
                     TLKLPYSDERVIHFGLIPRSHRGLFVFNELPDLQARIQVALFNILQEGDIQIRGFKIR
                     LPLDIQFVFTANPEDYTNRGSIVTPLKDRIDSQIITHYPRSIEVAKKITEQEARVREE
                     QKVKIGIHDFLRDLLEQIAIEARKSEYVDEKSGVSARLTISGMENLYSAAERRTLINN
                     EEKTHIRISDFAGVIPSITGKIELVYEGEQEGPGIVAQSLLGKAVRTQLVNYLPDPEK
                     SRKQKESNPYSTIISWFREGNTLDLLNDESTREFRKKLDSVPGLERLVTTHARPGDKE
                     LKYLFMEFALHGLAEFSLLSKNLLSSGSSFKDLVSNLFTMPDEGDKGEEDEDFYK"
     gene            complement(6140..7237)
                     /locus_tag="VGA21_00315"
     CDS             complement(6140..7237)
                     /locus_tag="VGA21_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010611816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX9648973.1"
                     /translation="MTGYRFTKFIPQEQKQKGSFKGMFSIFMQLLTITSGDVDEALSW
                     MNELDNQYGITTDEYGMGDFIEDLKKKGYLDKDSKTGAFRITARAEQSIRKNALEEIF
                     GKLRKTRRGDHTTIHSGQGDEPGTDRRDYQFGDTPDQISMTDSLRNAQINHGIGDFRL
                     TENDLEIVETESKSQTSTVLMIDVSHSMILYGEDRITPAKKVAMALSELITQKYPKDT
                     LDILVFGNDAWQIEIKDLPYLKVGPYHTNTIAGLELALDILRRRKTPNKQIFMITDGK
                     PTCMKVGIKYYKNSFGLDRKIVTKTLNLAAQCRKLSVPITTFMIASDPYLKSFVREFT
                     AANNGNAYYSSLQGLGHLVFEDYKRNRRKNF"
     gene            complement(7308..8450)
                     /gene="bshA"
                     /locus_tag="VGA21_00320"
     CDS             complement(7308..8450)
                     /gene="bshA"
                     /locus_tag="VGA21_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009583631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl L-malate synthase
                     BshA"
                     /protein_id="HEX9648974.1"
                     /translation="MKIGIVCYPTFGGSGVVATELGKALAREGHNIHFITYSQPTRLD
                     FFTENLFYHEVDIMNYPLFEYPPYELALASKLVDVVRHEKLDLLHVHYAIPHATAAYL
                     AKQILKSDNISIPIITTLHGTDITLVGKDPSYEPVVTYSINHSDGVTAVSQNLKDDTY
                     RHFKINKEIEVIPNFIDLNRFKKHKKDHFKKAICPNDEKLIVHTSNFRKVKRVDDAVR
                     VFSKIREQIPAKLLLVGDGPERASVEQLCRELGYYDEVRFLGKLEAVEEVLSVSDLFL
                     MPSESESFGLAALEAMACEVPVVASNAGGIGELIIHGVSGFMSNVGDIDDMSKNALYI
                     LKDDQLPKFKINALNRAMDFDVTNILPKYELFYNKIIESAVQLQKV"
     gene            complement(8488..11433)
                     /locus_tag="VGA21_00325"
     CDS             complement(8488..11433)
                     /locus_tag="VGA21_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009583630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HEX9648975.1"
                     /translation="MLKFLRLFALLLIGSHSIAQNPAFTSKESWVDSVFSSMSHEEKI
                     GQLFMIATWSNRGEDHQQEVEELITKYHIGGLIFFQGGPVRQVNHTNYYQSLAKIPLW
                     IGMDLEWGLGMRLDSTISFPKQMTLGAIQDDQLIYEMGYEIARQMKLIGVHMNYAPVV
                     DVNSNPMNPVIGFRSFGENKFKVAEKGIQYMQGMQDNGVMATAKHFPGHGDTDKDSHL
                     TLPVIKHKAARMDSLELYPFYQLFDRGIMSTMVAHISVPSLDNNPRQPTTLSYKVVNG
                     LLKNKMNFKGVVFTDAMNMKGIADRTKPGDADLKALMAGNDVLLYSIDIPKAVGRISR
                     AVLRKQINQIEIDSRVKKVLSFKYDLGLHRQQNFTTNNLLDNLNSPDAKALRLKLFEE
                     AVTVVKNENNLLPFTVLDTLHFASLSINTNGETTFQKTLNTYAPFQHYIIKDIPVSDD
                     AYADLLDKLEHFDVVVAGIHNMSQYQSRGFGLREPDMKFLKELNRRTRVVLVVFGNPY
                     SLKNFSEFDNIIITYEDTGDTEIIGPEVIFGAIEAKGKLPVTASKEFIAGQGIVTRSL
                     QRLGYDAPENQGMDSRVLQKIDQIAHEAIENGATPGCEVLIARNGRIIYDKTYGHFSY
                     DSLKEVNNNTIYDLASITKVTATLQTVMFLYGQGLMDLDKKAASYLPELKATNKKDMI
                     IRDILTHQAGLWPFVPFWRKTQGTKLDSIYYRNEYSKEYSIQIAPNLYALNGLQDSLW
                     KWTLNARVRDKTYRKPYDYTYSDMGYYILQKISERLLNQSMPDFLDQNFYAPMGMNSM
                     GFLPLCRFPEDIIAPTEFDTSWRLSHVRGMVHDQGAALYGGVAGHAGLFSNANDLAKI
                     MQMNLQGGNYGGIKFLEPGVIQSFITKQYMPSRRGIGWDKPTDNAQGPTSRYASFDTF
                     GHTGFTGTATWADPEFGLVYVFLSNRINPSAENRILLDENIRSRIHDIVYESIWSYRR
                     SFHQNGETE"
     gene            11498..11851
                     /locus_tag="VGA21_00330"
     CDS             11498..11851
                     /locus_tag="VGA21_00330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9648976.1"
                     /translation="MKFPSIFRTPSHQRFHFEPRYYDPIKEDIKQRRSRIVKELEEGK
                     TLDGEFKSQIKGNFNGRFYKRTTGTTSAGLLQFFIMALLFGGIFGYIYFGEMIFYFLM
                     PFVALFVYLRIRRIF"
     gene            11855..13678
                     /gene="mutL"
                     /locus_tag="VGA21_00335"
     CDS             11855..13678
                     /gene="mutL"
                     /locus_tag="VGA21_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009186111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEX9648977.1"
                     /translation="MSDIINLLPDSLANQIAAGEVVQRPSSAVKELMENSVDAGAKKI
                     QVIVKDAGKSLIRIIDDGQGMSETDARMSFERHATSKIHSAEDLFAIATMGFRGEALA
                     SIGAVAQVEMKTRLGKSELGSCLIVEGSEVKSQIADGCPIGTSISVKNLFYNVPARRN
                     FLKSNSVEMKHIVDEFQRVALAHPEIEFSLFHDDLEIYQLPAGKLSQRIVDVFGKSYR
                     EQLAACEEETTHINIKGYIGKPEFAKRTRGEQFFFVNDRFIKNPYLNHAVLNAFEGLI
                     PEGSFPFYILFISIDPASIDVNVHPTKNEIKFEDERTVYGIVRAAVRQALASHNLAPS
                     LDFEQDVNFLSRKMGASSEKQSRADRAYYQFKNVKKAGGDDWEKLFEGISDRLNVEGR
                     KDKPDLELTFESSLNQELPAGEQTPDTSVSPFQLHNRYIIKQVKSGMMIFDQERVHQR
                     ILFEKFNQNLENKSGNTQQCLFPQTVHLNISDYSLVMDMSEEIRSLGFEFEVMGKDSL
                     VINGIPSDLSGGNEKEIFEGLLEQFKENKTELSLPTKENLAMAMAKQSSIKHGKSLSK
                     HEMDSLIDKLFGCSNPNYTPDGQPVYIIFDLEKIIGLFKRN"
     gene            13699..14487
                     /locus_tag="VGA21_00340"
     CDS             13699..14487
                     /locus_tag="VGA21_00340"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009583627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEX9648978.1"
                     /translation="MVKGLLIINVAVYLITMMTGSSDANLNNYLALYQINSPYFQPYQ
                     LFTYMFAHSSGSFTHILFNMIGLVVFGGFLENFWGSNRFLVFYLGTGIGAAIIYSSVN
                     FIQNNSLENKIEAYSMNPTPDGFNKIILGESRGLYNEWYDFIREYGRNSNDATYISIS
                     KERLAQLYKYKQRGSMVGASGAIYGLLMACAMLFPNLMIMLLFPPIPIKMKYLALILG
                     GMALYSSLQPQEGDNVAHLAHLGGMIFAYIMIVVWRKQGKDYSG"
     gene            14492..15406
                     /locus_tag="VGA21_00345"
     CDS             14492..15406
                     /locus_tag="VGA21_00345"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009583626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEX9648979.1"
                     /translation="MNGGGILDDFKNAFNRPNNGHIQLILINVVIFLVLIISKVFIDL
                     SLGTEAFRSVLTQIAMPSHTETFLIKPWTLFSYMFVHIGFFHILFNMLFLYWFGMVIK
                     EFLGSNRVIALYILGGIAGGLSYILIYNLIPFYADRVENSILFGASAGVFAVVVGAAT
                     FMPNYTFFLLFIGPVRIKYIALFYVVLSFANTTGPNAGGELAHLGGALVGFLFIKQLN
                     RGTDWGKPVLSVIHFIRSFFVKQSNIRVTYSRKEKASAGASRQPAKKSASRVEQDEID
                     EILDKISQSGYDSLSKEEKEKLFNASKK"
BASE COUNT         4494 a         3134 c         3206 g         4649 t
ORIGIN      
        1 gagttccgat gaaaacatga tttccttcct gttcaagaaa tccggagtaa acaggcaaac
       61 ctttctggaa ggtctggaag acatcgtgac caaataccca aaggtaagcg gtcagcaacc
      121 ttacctttcc aacgatagca ataaggtatt acaacttgcc aatgattttt taaaggaatt
      181 taaagatgaa tttgtcgctg tggagcacct ttttcttgga atattttccg gtaatgataa
      241 agttgcggtg ttgatgaaac aatccgggtt tacaaaacct gttctcatta agtcaattaa
      301 agaacttcgc ggaaacgccc gggtaacaga ccagaatgcg gagtccaaat atcgatcgct
      361 tgaacgctat tcaaggaatc tgaatgaact tgcagcaaaa ggcaagattg atcccgttat
      421 tggcagagat gatgaaatcc gaagagtttt acaaattctt tcaagacgaa caaagaacaa
      481 tcccattctc ctgggagaac ctggcgtagg taaaacggca atcgtggaag ggctggctca
      541 aaggattgtt gatggagatg ttccagaaaa tctgaaatca aaaattattg tctcgcttga
      601 catgggactc ctgattgccg gtgcaaaata taaaggggaa tttgaggaaa gactgaaggc
      661 ggtaattaaa gaagtcaccg attccaatgg tgaaattatc atgttcattg atgagattca
      721 taccctgatc ggtgccggat caggagggga aggagccatg gacgctgcca acttgctaaa
      781 acctgctctg gcacgagggg aattgcatgc aattggagcc actactttga aagaatatca
      841 gaagtttatt gaaaaggaca aagccctgga aagaagattt cagacggtta tggtggatga
      901 gccaagcctt gaagattcta tttctatcct gcggggtatc aaagacaggt atgagttaca
      961 tcatggagta agaataaaag atgacgcggt tattgccgca gtggaacttt ccagccggta
     1021 tataacagac cgctacctgc ccgacaaagc tattgacctg gtagacgaag ctgcttccaa
     1081 attgagactt gaaattgatt cattaccgga agaacttgat gaattgcaaa gaaagattat
     1141 gcaattagag attgaacggg aagctatccg gcgggaaaaa gataaagaca aggaaagaac
     1201 tttgagtaaa gccattgctg aacttggtga atccagggat catttgaaag caaagtggaa
     1261 gaatgagaag gaaattatta gtggcattca acatgaaaaa gaaaatattg aaaaatacag
     1321 acttgaagcg gagagagcag aaaaagcagg cgattacggc cgagtagcgg aactacgata
     1381 cgggataatt caggaagccg aagcaaaact tgagttgttg aaaaagcaaa tgaacgaaat
     1441 tcaggctgaa agtcctttgt taaaagagga agtagataat gaagatattg ccgatgtagt
     1501 tgcaaaatgg actggaatac cggtgagtaa aatgttgcaa agcgatcgcg aaaaactttt
     1561 gcaccttgaa gatgagttgg gaaaacgtgt tgcaggtcaa agtgaagcga tccaggcgtt
     1621 atcagacgcc gtgcgcagaa gcagggccgg cttacaagat ccaaaacgcc cgattggctc
     1681 gtttatcttt ctgggtacaa cgggagtggg taaaaccgag ctttcaaaag cactggccga
     1741 gtatcttttc aatgatgaaa atgcaatggt tagaattgat atgtctgagt accaggagcg
     1801 acattctgta agcaggttgg tgggagcccc tccgggatat gtaggctatg atgagggcgg
     1861 acagctgacg gaatcggttc gaagaagacc ttacagcgta gttttacttg atgaaattga
     1921 aaaagctcat ccggatgtat ttaatatcct gctccaggta ctggatgatg gcagattgac
     1981 cgacaacaag ggcagggttg ccaatttcaa gaataccata attatcatga caaccaatat
     2041 cggttcacac atcatccagg aaagttttga gcgaatgaat gaggaaaatg cagaagaagt
     2101 tatggaagca acaaggttgc aagtgatgga gctgctgcgt aaatcagtgc ggccggaatt
     2161 cctgaaccgg gttgacgaaa ccattatgtt caggcctttg acgaaagaag acattcgaaa
     2221 aatcgttgaa ttgcagtttg gcattataaa aaagagactt gatgaagcag gtgtaaaagc
     2281 tgaaatttca gacaaggcta ttaactacct ggcaaaagcc ggctttgacc cacagtttgg
     2341 tgccagaccg ttgaaacgag tgatgcaaaa attgattttg aatgaattga gtaaacagat
     2401 tcttgaaggc aaaattgcga aagacggaat tattggaatt gatgttggag ataacaatca
     2461 gctggagttt ttgaacctgg ataaggtaga gctcagttag taattagctc caaaaataaa
     2521 tcttgaagga agagtatggt agaaatatcc atcacatcaa ttcgttggct aagttggcta
     2581 actcggacct ttctcctttc tccagagtaa tgtgcgcata cagattgtga ttcttgatgc
     2641 gatcaatgat ataagataaa ccgttgcttt gtgaatccag gtaaggagtg tcaatttgat
     2701 taatgtctcc tgtaaaaatc aacttcgtat tttcccctgc ccgggttata atggttttaa
     2761 cttcatgggg agtgagattt tgcgcttcat ccacgataaa aaaaatgttg gataaacttc
     2821 ttccccggat atatgccaaa ggcgtaatta ccagtttctc ctgattcacc atttctgtaa
     2881 tttttgaata ctccttatcg gtttccttgt attgattctg aatgaatttc agattatccc
     2941 ataatggttc catgtacggg ttcaattttg atttgatatc tccgggaaga tatccaatat
     3001 ctttattgct gaggggcacg atcggcctgg ccagataaat ctgtttaaag tcacgctttt
     3061 gttccagtgc tgaagacaaa gccagcaagg ttttaccagt tcctgccaca cctataatgc
     3121 taacgagtct taccttagga ttcagcaagg catgaatagc aaatgtttgc tccgcattgc
     3181 gcggttttat tccgtaaatc attcgcttct ctaccttctc tactaattga ctttccggat
     3241 tataataagc cagcacagat tttttatcat ttttaataat gaagtagtta ttggcaatga
     3301 gtatagactt ataaagaatt tcttcaggag cacagaaacc tttttcataa attttattaa
     3361 tcacttcagg aggaacttca ttaagttcag acttacctga aaagagtgaa tttacatttt
     3421 gaatcttgcc ggttgtataa tcttcagcgg gaaggttaag tgacttagct ttaagcctta
     3481 gattaatgtc tttggaaacc aggatcactt tccggtttgg gttttctctc tgaagtgcaa
     3541 gtgcggaatt taaaatccta tggtcagctt tttcttcatc aaaaatcttt actgcatcaa
     3601 caccattggt ggtattcatc accactttaa acttgccttt tgatcgcccg tttaaaggaa
     3661 cccaattctg aagggtgtgt ccttgtgcca gattatccag cagacggata aactctcttg
     3721 cttcgtagtt aatgatatca tttccttttt tgaattgatc cagctcttcg agcacggtaa
     3781 tgggaatggc gacgtcatgc tcgtcgaaat tcattataga attgctctcg taaaggataa
     3841 cagaagtgtc taaaacaaat attttcccct ggcttcttcc cttggccata tttcagcctg
     3901 taattttcag tttgatgtca aatttatcta aaaaaacaat taagctggca ttgcaatgct
     3961 cttattgttc ggttgctatt ttatttaatc cgaatacatc atgcttttct cccggcacca
     4021 ccattaagac attcgctaaa accaacaaaa ttaattttcg cgcagcctgg tagggcttaa
     4081 gccttgccac caaagcatat tgatcaattg taataaaatt attttcttcc agatagttca
     4141 tcagaatttt ttcactgtca ccgaaataga attgaatgtc tttgcctttc ttttgccgct
     4201 ccataatttc aacaacctcc cggctggctt ttacactttt atcccctacc cggataaagt
     4261 attcatcttt accgggagcc agccttacgg aatgcggccg tcgttcactt ttatctatgg
     4321 tgtatcgcaa tacgaaccgg ttttctgaaa caggaatttg ttcaacatga aaagaaatga
     4381 caggcttgca gaaagattct atggcatttt caagagcaaa gcggtgctca tcgggatact
     4441 ttaacccgct gatttctctg ttgtcatcaa cgccgataag tagttctcct ccctgggtat
     4501 tggcgaaggc agtaatttcc ttgatcacct tttcaggata atccactttc ttcttaaact
     4561 caaggtggtc attttctccc ttgcgaacca atgaccttag ttccctgatg gtcattcttt
     4621 atttataaaa atcttcgtct tcttctccct tatcgccctc gtcaggcatg gtaaataaat
     4681 tactcaccaa atctttgaac gaactgccag acgaaagcag atttttgctc aataaactaa
     4741 attccgcaag tccatgcaag gcaaattcca tgaataaata cttcaattcc ttgtcgcccg
     4801 gccgcgcatg agtagtaacc agtctctcca gccccggaac actatccagc ttctttctga
     4861 attcccgcgt gctttcatca tttagcaaat caagtgtgtt accttctcta aaccagctaa
     4921 ttatggtaga atatggattt gactcctttt gctttcttga cttttccggg tcaggcaggt
     4981 aattaaccaa ttgggttctg acggccttcc ccaataaaga ttgcgcaaca atacccggac
     5041 cctcctgctc cccttcatag acaagctcta tttttcccgt gatggaggga attacccctg
     5101 cgaaatcaga aattctgatg tgagtttttt cttcattatt gattaaagtc cttcgttctg
     5161 ccgcgctgta caaattttcc attcctgaaa tagtcagcct ggcacttaca ccgctctttt
     5221 catccacata ttcacttttt cttgcttcga tagcaatttg ctccagtaaa tccctgagaa
     5281 aatcatgaat cccaattttt actttttgtt cctccctgac ccgggcctcc tgttcagtaa
     5341 tttttttggc aacttcaatg ctcctgggat aatgggtgat tatctggctg tcgattctgt
     5401 ctttcagtgg agtaacaata ctacccctgt ttgtatagtc ttcaggattt gccgtaaaaa
     5461 caaattgtat atccaaagga agtctgattt tgaaaccgcg aatctggata tctccttcct
     5521 gaaggatatt aaacaaagct acctggatgc gcgcctgtaa atcgggaagt tcgttgaaaa
     5581 caaataaccc ccggtgtgaa cgcggaatca atccgaagtg aatcacccgc tcatctgagt
     5641 aaggtaattt taaggtggct gcctttatag gatcaacgtc gccgattagg tctgcaatag
     5701 atacatcggg agttgccagt ttttctgtat atcgttcgcc gcgatgcatc cagctaacag
     5761 gtgtggcatc tttacattct tcaataatat ccctggcctg cctgctgata gggttaaaag
     5821 gatcatcgtt taattcagtg tcttttatca ccgggataaa ttcatcaagc aaatcaatca
     5881 tgagccgtgc aagtcttgtt ttggcttgcc cgcgtaagcc aagcaagtta atatcgtgca
     5941 tggagagaat tgcgcgctct aaatcaggaa taacagtctc ttcatatccc cagattccat
     6001 caaatacttt ttcctttttt ttgagttttg cgattaaatt atcccgaagc tccagcttca
     6061 cagatttggt tttgtagccg gatcttttaa gatcacctaa cgtactaatt ttactataat
     6121 caatatcctt gctcattccc taaaaattct ttctcctgtt tcgtttataa tcttcaaaaa
     6181 ccaaatgacc taacccctgc aagctgctgt aatatgcatt cccattattt gccgctgtaa
     6241 attccctgac aaacgatttg aggtaaggat cggaagcaat cataaatgtg gtgatgggta
     6301 cagataattt gcggcattgc gcggccaggt tcaatgtttt cgtcacaatc tttctatcca
     6361 gtccaaagct gtttttgtaa tacttgatac ctactttcat gcaagtgggc ttaccatcgg
     6421 taatcatgaa aatttgctta ttgggagttt tcctgcggcg aagaatgtca agtgcaagct
     6481 ccaggcctgc tattgtattg gtatgataag gcccgacttt tagatatggc aagtctttaa
     6541 tttcaatttg ccacgcgtca tttccaaaca caagaatatc taaggtatcc tttgggtatt
     6601 tctgcgtaat caattcggaa agcgccattg ccactttctt agcgggagtt atccggtctt
     6661 cgccatacaa aatcatggaa tggctgacat caatcatcag tacggtggag gtttggcttt
     6721 ttgattcagt ttccacaatt tccaagtcat tttccgtaag ccggaagtct ccaataccat
     6781 gattaatctg cgcatttcgc agagagtcgg tcatggaaat ctgatccgga gtatctccaa
     6841 attggtagtc tcgcctgtca gtccccggct catcaccctg tccgctatgg atagtcgtat
     6901 gatcacccct ccgcgttttg cgcaatttcc caaaaatctc ctccagcgca ttctttctaa
     6961 tcgactgctc tgctctggca gtaattctga atgctcctgt tttggaatct ttatcgagat
     7021 atcctttctt tttcaaatcc tcaatgaaat ctcccatccc atattcatcc gtagttatgc
     7081 catactggtt gtccaactca ttcatccagc ttaatgcctc atcaacatca ccggaagtaa
     7141 tggtgagtag ttgcatgaat atggaaaaca ttcctttaaa agaacctttt tgcttttgtt
     7201 cctgtggaat gaatttggtg aatcgatatc ccgtcattgc tgtggactaa gctaaataaa
     7261 ttgatttaca gatttcagaa aatccttata aatcctccga taaatgatca gactttttga
     7321 agttgaaccg cagactcaat tattttatta taaaataatt cgtatttggg aagaatattg
     7381 gttacatcaa aatccatagc cctgttgagc gcattgattt tgaattttgg caattgatca
     7441 tccttcaaaa tataaagagc gttttttgac atgtcatcaa tatcgcctac gttactcata
     7501 aacccgctca ccccatgaat aatcagttct ccaatacctc cggcatttga agcaactacc
     7561 ggaacttcgc aagccatagc ttcaagggcg gccagtccaa aactttcact ttcggaaggc
     7621 atcagaaata aatcggatac agagagtact tcttccactg cttccaattt gccgagaaat
     7681 ctaacttcgt cataatatcc cagttcgcgg cacaattgct ccacagaggc ccgctcagga
     7741 ccatcaccca ccagcaatag ctttgccgga atctgttccc ggatttttga gaatactctg
     7801 acggcatcat ccactctttt cacttttctg aaatttgaag tgtgaacaat cagtttttca
     7861 tcgttgggac aaatagcttt tttgaaatga tcctttttgt gttttttaaa ccgattcaga
     7921 tcaatgaagt ttggaattac ttcaatctct ttatttattt tgaaatgtcg gtacgtatca
     7981 tccttaaggt tttgtgatac tgccgtaacc ccgtcagagt gattaattga ataagtaaca
     8041 accggctcgt atgaagggtc tttgcccacc agtgttatgt ccgttccatg taaggttgtg
     8101 atgataggaa tggaaatatt atccgatttc agaatttgct tggcaaggta agctgctgtt
     8161 gcatgtggaa tggcataatg aacatgcaat aagtccaatt tttcatgtcg cacaacatct
     8221 accaatttgc ttgccagggc taactcgtag ggtgggtatt caaacaaggg atagttcatg
     8281 atatccactt catgataaaa caggttctca gtgaaaaagt ccagtcttgt gggctgagag
     8341 taggtaataa agtgaatatt atgaccttcc ctggcgagtg cttttcccaa ttcggttgca
     8401 actacaccgc taccaccaaa agtggggtag caaacaatgc ctatcttcat tttaatctat
     8461 ctatttatct attaacagga taaatcgtca ttcggtttca ccattttgat gaaaagatct
     8521 gcggtaactc caaattgact cataaacaat gtcatgaatc cgcgatctga tattttcatc
     8581 gaggagtatc cggttttctg ccgatggatt aatccggttg gagaggaaaa cataaaccaa
     8641 gccaaactca gggtctgccc aggtggcagt tcctgtaaag cctgtatgcc caaaagtatc
     8701 aaaagaagca tacctggaag ttggaccctg ggcattatct gttggcttat cccacccgat
     8761 tccacgcctg ctgggcatat attgtttcgt aataaagctt tgaattactc ccggttcaag
     8821 aaattttatt cctccgtaat ttccgccttg cagattcatt tgcataatct tcgccaggtc
     8881 attagcattg ctgaatagcc cggcatggcc cgccactcca ccgtaaaggg cggcaccctg
     8941 gtcatgaacc attcctctta catgagacaa cctccaggaa gtatcaaact cggtaggcgc
     9001 aataatgtct tcgggaaatc tgcacaaggg caaaaagccc attgaattca ttcccatggg
     9061 cgcgtagaaa ttctgatcca gaaaatcagg catggactga tttaataacc gctccgagat
     9121 tttttgaagg atataatatc ccatatcgct atatgtatag tcataaggtt ttctgtatgt
     9181 cttatcccgg acccgggcat tcaaagtcca cttccaaagg gaatcctgga ggccgtttaa
     9241 ggcgtataaa ttaggggcta tttgtattga atattccttt gaatattcat tccggtaata
     9301 aattgaatct aattttgtac cctgcgtttt tcgccagaaa ggaacaaagg gccagagacc
     9361 tgcctgatga gtcaggatat ccctgataat catatccttt ttattggtgg ctttcaattc
     9421 aggtaaataa gaagcggctt tcttatccag atccatcaat ccctggccat aaagaaacat
     9481 cacggtttgt aaagttgccg tgactttagt tatagatgcc aggtcatata tggtattatt
     9541 attcacctct ttcaaactat catatgaaaa atgtccatac gtcttatcgt agataatgcg
     9601 cccatttctt gcaatcaaaa cttcgcatcc cggagtagcc ccattttcta tggcctcatg
     9661 cgctatttga tcaatttttt gaagtactct ggaatccatc ccctggtttt caggtgcgtc
     9721 gtaccctaat cgctggagtg atcgggttac aattccctgc cctgcaataa attccttgga
     9781 tgccgttacc ggtaatttcc ctttagcttc aattgctcca aatattactt caggtccaat
     9841 tatttccgtg tcacccgtat cttcgtaggt gatgatgatg ttatcaaatt ccgaaaagtt
     9901 cttcaggctg tatgggtttc caaatactac aagtacaact cttgtgcgcc ggtttagctc
     9961 tttcaggaat ttcatatcag gttcccgcaa tccaaatcca cggctttgat actggctcat
    10021 attgtgaata cctgcaacta ctacatcaaa atgttcaagc ttatccaaaa gatcagcata
    10081 agcatcatcg cttaccggaa tatctttgat gatgtaatgc tgaaatggag catacgtatt
    10141 taaagttttt tgaaaagtgg tttcgccgtt cgtatttata ctcaatgacg caaaatgcag
    10201 agtgtccaga acggtgaatg gaagcaaatt attttcattc tttacaacgg taaccgcttc
    10261 ctcgaataat tttaatcgta acgcttttgc atcaggggaa ttaagattat ccagcaagtt
    10321 attggtggtg aaattttgtt gccggtgaag tccaaggtcg tacttaaatg acagcacttt
    10381 cttaaccctg gagtcaattt cgatctgatt tatttgcttc cttaacaccg cgcgtgagat
    10441 gcgacctact gcttttggaa tatcaataga atagagtaac acatcattac ctgccatcaa
    10501 tgctttcaaa tctgcatcac ccggtttagt tcggtctgca attcctttca tattcatagc
    10561 atcggtaaat acaactcctt tgaaattcat tttatttttc agaagcccgt ttacaacttt
    10621 atacgatagc gttgttggct ggcgtggatt gttatccaaa gaaggtacac ttatatgggc
    10681 aaccattgta ctcataattc ccctatcgaa aagctggtaa aaaggatata gctcaagcga
    10741 atccattcgc gcagctttgt gtttaataac aggcagggtg aggtgactgt ccttatcagt
    10801 atcgccgtga ccgggaaaat gtttcgctgt tgccattact ccattatcct gcatcccctg
    10861 catatactga attccctttt cagcaacttt gaatttgttt tcgccaaaag agcgaaatcc
    10921 gataacggga ttcataggat tgctattgac atcaactacc ggagcatagt tcatatgcac
    10981 cccgatcaac ttcatttgcc tggctatctc gtaacccatt tcatagatta gttgatcatc
    11041 ctggatagca cctaaggtca tctgctttgg gaaacttatg gtactgtcaa gcctcattcc
    11101 caggccccat tcaagatcca tcccaatcca taagggaatc tttgcaagag attggtaata
    11161 atttgtgtgg tttacctgtc tgacaggtcc gccctggaaa aaaatcaggc cgccaatgtg
    11221 atactttgta atcaattcct ccacttcctg ttgatgatct tctcctcgat tagaccacgt
    11281 tgcaatcatg aaaagttggc caatcttttc ttcatggctc atggatgaaa aaacgctgtc
    11341 aacccaactt tcttttgagg tgaacgccgg attttgagca atcgaatggc tgccaatcaa
    11401 cagaagggca aacaatcgca aaaattttaa catgggcaaa gtagagttta ttattaggat
    11461 aaaaatagct tatttttgaa tgaaaagtgg attataaatg aagttcccct ccatcttcag
    11521 aactccttcg catcaacggt ttcattttga acctcgttat tatgatccca ttaaagagga
    11581 tattaagcaa cgacgatcca ggattgtgaa ggaactggaa gaaggaaaaa cattagatgg
    11641 tgaattcaag tctcagatca aaggaaattt caacggaagg ttttataaaa gaactaccgg
    11701 aactacttca gccggcttgt tgcaattttt tattatggct ctgcttttcg gaggaatttt
    11761 tggctacata tatttcggtg aaatgatatt ctattttctg atgccttttg tagccctctt
    11821 tgtttatctc aggatcagaa gaatatttta gcaagtgtcc gacattatta atttacttcc
    11881 tgattcgctg gctaaccaga ttgcagccgg agaagttgta cagaggcctt cttcggctgt
    11941 gaaggaactt atggagaatt ctgtggacgc cggtgcgaaa aaaattcagg tgattgtaaa
    12001 ggacgcgggc aaaagcttaa tcaggattat tgacgatggg caaggtatgt ccgaaacaga
    12061 cgcccgcatg agttttgaaa gacatgcgac ttcgaaaatc cattcagcgg aagatctttt
    12121 cgctattgct actatgggat ttaggggtga agcccttgca tccattggcg cggttgccca
    12181 ggtggagatg aaaacccggc ttggcaaaag tgagttggga agctgcctga tagtggaggg
    12241 ttcggaagtc aaaagtcaga tcgcggatgg atgtccgatt ggaacttcca tcagcgtaaa
    12301 gaatcttttt tataatgtgc cggcccggcg taattttctc aagtcgaatt cagtggagat
    12361 gaaacacatt gtagatgaat ttcagcgagt agcgttggcg catcctgaaa ttgaattttc
    12421 cctcttccat gatgacctgg agatttacca actgcctgcc gggaaattga gtcagagaat
    12481 tgtagatgta tttggtaaat cttaccggga acaactggca gcttgtgagg aggaaactac
    12541 tcatataaat ataaaaggat acatcggcaa gcctgaattc gcaaaaagaa cccgcggcga
    12601 acaattcttt tttgtaaacg acaggttcat taaaaatcca tacctgaatc atgccgttct
    12661 caatgcattt gaaggattaa tccctgaggg aagttttcca ttttacattt tatttatttc
    12721 cattgaccca gccagtatcg atgtaaatgt tcatcctaca aaaaatgaaa taaagtttga
    12781 agatgaaaga acagtgtatg gtattgtacg tgccgctgta aggcaggcgc tggcaagtca
    12841 taacctggca cccagccttg attttgaaca ggatgtgaat tttctttccc ggaaaatggg
    12901 cgcgagttca gaaaaacaat ccagagcaga ccgtgcctat taccaattta agaatgtgaa
    12961 gaaagccgga ggtgacgatt gggagaaatt atttgaagga atctctgaca ggctgaatgt
    13021 tgaaggcagg aaagataaac cggatttgga attaactttt gaaagttcac tcaatcagga
    13081 actaccggca ggtgaacaaa caccggatac ctcagtcagc ccgtttcagt tgcataaccg
    13141 ctacattatc aaacaggtga aatcgggaat gatgatcttc gatcaggaaa gggttcacca
    13201 aagaattcta tttgaaaaat tcaaccaaaa tcttgaaaat aaaagcggca atactcagca
    13261 atgtctgttt ccccaaacgg ttcatttaaa catttcagat tattctctcg ttatggatat
    13321 gagtgaggaa ataagatcgt tgggatttga gtttgaagtg atgggaaagg attctttggt
    13381 gatcaacggg attccatctg atcttagcgg gggaaatgaa aaagaaattt ttgaaggatt
    13441 gttggaacaa ttcaaggaga ataaaactga attgtcttta cccactaaag aaaatcttgc
    13501 catggcaatg gcaaaacaat cctcaattaa acacggcaaa tcattgtcga agcacgaaat
    13561 ggattcttta attgataaac tatttggctg ctcaaatcct aattataccc cggatggtca
    13621 accggtttat attatctttg acctcgaaaa aataatcggc ctatttaaga gaaattaatg
    13681 tttggaagat taacaccgat ggtaaaggga ctgctgataa ttaatgtagc agtttattta
    13741 atcactatga tgaccggatc gagcgatgca aatctgaata attacctggc cctttatcag
    13801 attaattcgc cttattttca gccctaccaa ttattcactt acatgtttgc acacagctca
    13861 gggagtttca cgcatatttt gtttaacatg ataggattgg tggtgttcgg tggctttctg
    13921 gaaaacttct ggggttcaaa ccggtttctt gttttttacc ttggaactgg tatcggggct
    13981 gcaattatat acagttcagt taattttatt caaaacaatt ccctggaaaa taagatcgaa
    14041 gcctacagta tgaacccaac tccagatgga tttaacaaaa ttattctggg tgaatcaaga
    14101 ggtttgtata atgaatggta tgatttcatc agggagtacg gccggaacag taatgatgca
    14161 acatatattt ctataagtaa ggaaaggctt gcacagttat acaagtataa gcaaagagga
    14221 tcgatggttg gagcatcagg agctatatat ggattactta tggcctgcgc tatgttattt
    14281 cccaatctaa tgattatgct tttgttcccg ccgattccaa ttaaaatgaa atatcttgca
    14341 ttaattttgg gtggaatggc attatattca agtctacaac cccaggaagg cgataatgta
    14401 gctcacctgg ctcacctggg tggaatgatt ttcgcatata taatgatagt ggtctggaga
    14461 aagcagggaa aggattattc aggataaatt tatgaatggc ggaggaattt tagacgattt
    14521 taagaatgct tttaacaggc ccaacaacgg ccacatccag ttaatcctga ttaatgttgt
    14581 tatattcctg gtactaatta tttccaaagt atttatcgat ttgtctttgg gtactgaagc
    14641 cttccggagt gttctcactc agattgcaat gccttcccac acagaaacct ttctgataaa
    14701 accctggact ttgttctctt atatgtttgt acatataggt ttctttcata ttctatttaa
    14761 catgcttttc ctgtactggt ttggaatggt tataaaggaa ttcctggggt caaaccgggt
    14821 tattgcgttg tacattctcg gtggtattgc aggtgggctc tcttacattc tgatatataa
    14881 tctaattcca ttttatgcag acagggtgga gaattcaatt ttattcggag catccgccgg
    14941 agtatttgcg gtagttgtcg gagcagctac ctttatgccg aattatacat tcttcctgct
    15001 atttattggt ccggttagga tcaaatatat tgccctgttc tatgttgtgc tgagtttcgc
    15061 caatactaca ggacccaatg ccggcggaga actcgctcat ctgggcggcg cattggttgg
    15121 attcttgttc atcaagcagc taaaccgggg aaccgactgg ggtaaaccgg ttttatcagt
    15181 aatacatttc attagaagct ttttcgtaaa gcagtcaaac ataagagtta cgtattccag
    15241 aaaagagaaa gcaagtgccg gtgcgtcgcg acaacccgca aagaaaagcg cttccagggt
    15301 ggagcaagat gaaattgatg aaatcctgga taagatttca caatcgggat atgatagcct
    15361 ttccaaagaa gaaaaagaaa aacttttcaa cgccagcaag aaatagttaa acaattagca
    15421 agtaggtaat taggtaatta ggtaattagg taattaggta attaggtaat taggtaatta
    15481 gtt
//
LOCUS       DASWHX010000008        15315 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_743651, whole genome shotgun sequence.
ACCESSION   DASWHX010000008 DASWHX010000000
VERSION     DASWHX010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 15315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15315
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_743651"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..524)
                     /locus_tag="VGA21_00350"
     CDS             complement(<1..524)
                     /locus_tag="VGA21_00350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9648980.1"
                     /translation="MKLMKLYSFLLMGVMLSTSMLFTSCSEDDDTDPLVGTYAFTLGT
                     MINTTNVVFPSATSIGTIAANDQTTTTLIIGQGLYASSPCATATNARTELRADGTLWN
                     VCAGGDAAEAQQGAWVIDAGRTQLTLTVTVEGVGSLDIVLSGFVDNGVTITGRIENFP
                     LGLDLNYALGSVLP"
     gene            704..3517
                     /locus_tag="VGA21_00355"
     CDS             704..3517
                     /locus_tag="VGA21_00355"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEX9648981.1"
                     /translation="MEIICLPEYKRSILMILFLAVFAIVTHAQTTITGTIKDENGEPL
                     IGVNIIMKDKLTGTVTDTKGTFFLQTESNPPFTLVFSYVGYETNEVDIRNQTIIDLNL
                     KEQVMLGQEVVISASRVEENILQSPVTIERIGILEIRENAATDFYDRLGSLKGVDMNK
                     QGFTFKVPNTRGFNGNTNYRVNQIVDGIDNAPPGLNFAAGNIFGLSQLDVESLELLVG
                     ASSALYGPGGMNGTLLMTSKNPFEYQGISAYVQTGIHHLAADYRNTPTPMVDANLRWA
                     KAWKNKFAMKVVASYIQALDWHADDYRDRNDLDDPNLTRETNAGYDGVNVYGDEAIVP
                     VNLQDLAPTVGDGVARQQGYQPGDPEYDAILQLIEDLFPDQIITRTGYKERDLVDYNT
                     SNFRIQTSMHYRLSEKVEAIFAGGYGNGTSVYTAQNRFSLRDFEIYQGKLELNSNNFF
                     VRAWGVKESSGKTYDAGATALLINEAWKPSQDWYTEFIQTFTTNLLLGNPVASSLATA
                     RTVADNRTPQGGILNPGLPTMPLPGTPEFESYFNDITSRTVDQQGSGIIDKTSMVNLE
                     FMYDFTGKIVFADILVGASDRVYNVNSEGTIFFDEPNDPIIQNQFGAYAQISKKLMDK
                     KLKFTGSVRYDKNDKYEGRITPRGSFVYSFGTDRRNNFRGSVQTAFRFASISDQWVDL
                     NVGPYQVIGGLPEVQAKYDFHIKDVYPLIGSNTLDSQPDLSNGPFVIPEFGPERVVAY
                     EIGYRGLFFDNRMLVDAYGYYNRFNGFLATQNLVMDPNTPDERRFQTTVSTDDPVSAY
                     GWALGIDYRFNRGYLLRGNVAHNTLEPLGDRPPGFESRFNSPNYRTNITVSNLHVINN
                     IGFSINWRWQNRFLWESTFGVGEVPAFHTLDAQVSFKLNALKSVVKVGGANLLNQYYT
                     TGFGNPAIGGLYYVTLTFDQFMN"
     gene            3536..4819
                     /locus_tag="VGA21_00360"
     CDS             3536..4819
                     /locus_tag="VGA21_00360"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX9648982.1"
                     /translation="MRRVILLPIVLYILTACTPAEPQILQAEVERIIGTLASDEMKGR
                     RTFSPEIDLASDFISGEFERIGLQKWDGLDSYKQEFYVFNFTVDSLVVKTDGKSLGMA
                     SATTTSENISWTEESNVASVFVGEKDTLQRTYGRYRRAGENVILWIHPAHQQLYTRYA
                     SFLATGSRTMTRAGGSTIVFAISESKPELFSVEFTASVTELPLANVVGYIEGNRKDEY
                     VLFSAHYDHIGIQRPVEGDSIANGANDDASGSTAVIMLADYFKAKSKPERSLLFVTFT
                     AEEIGGYGSRYFSQQLDPDKIVAMFNIEMIGKPATDGPNSAWITGFDKSDFGQILQKS
                     VEGTEYKFYPDPYPTENLFYRSDNATLARLGVPAHSISTTPIDVDPDYHQVSDEVKTL
                     NLEHLANTIKAISAGAATIISGEATPTRVNPANVN"
     gene            4823..5806
                     /locus_tag="VGA21_00365"
     CDS             4823..5806
                     /locus_tag="VGA21_00365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9648983.1"
                     /translation="MKIIPNNELNRDAWDLLIATSEQGNFYALSWYLDIVSPGWSAIV
                     EGTHQEYRIGIPLPVTRKWGFRILKQPLFCQQLGIYSITQPTQDVVAEIVNLITRKFN
                     YIIKYSFSNFNTPRLLSGSLSENLNVVLNLNQPYDVLKSNYKRDRKYRLMQVLNSNVF
                     TQESNSLEELICMFKENGEKKIYGGVSSTAYEMLHQIFYETATRGLANLIYAKVPPDK
                     IIAGALFIKFKNRITYMFNAGYSEFVKLNGRTLILDSVIRKYQNQPLLLDFECPGLKN
                     ISTFYRSFGGLDEIYLSLSQNHLPWPVKKIHAMRKKYYERDLYDKSARNGS"
     gene            complement(5847..6704)
                     /locus_tag="VGA21_00370"
     CDS             complement(5847..6704)
                     /locus_tag="VGA21_00370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9648984.1"
                     /translation="MKNLMCYLFCCLSIGALAQPATEVYIFDLQFGEGEMQIANPVNV
                     SNHKGYDNQPHFLEDGSLLYVSSINGQTDVYHYSNGSSKQITATPGGEYSPTPTPDSE
                     HFSTILLEPDGTQLLWQYNFDVKGNKPVLLAAGLKVGYHAWVSDHQIAAFVLGDPNTM
                     HLIDVSTGQSEIIGESIGRSLHRIPGTDYFSYIQNDDTGNSEIRSYDLKTGISELLIR
                     SVENSQDCAWTQNGTLIMGSGSKLFRWSKGMTEWKLIADISSFNLDGITRLAVNKDMS
                     KIALVVSEN"
     gene            6838..7854
                     /gene="holA"
                     /locus_tag="VGA21_00375"
     CDS             6838..7854
                     /gene="holA"
                     /locus_tag="VGA21_00375"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEX9648985.1"
                     /translation="MNLTPKEIIKQLKSGKYESIYFLMGAESFYIDEISNFIEESALD
                     DSQKEFNQTILYGMDVKISDILTHARRFPMMSERQVLIVREAQSVSDINREGGQKLLL
                     DYISNPLPSTILVFCHKNKTLDKRKSLYKALTKSSSVVMESAIIKEYKIPGWIKERIA
                     EKGLKITEKAVMMLTQNIGSNLERINNEIEKVSINIKTKTIDDNAIQTYVGINRDYNI
                     FELVNALGARNKVKAHEIAFYLGTHSKENPLVLIIGFVFSFFSKLLKLYGASGKTDKD
                     LYSILGTSYISDYKVAAGSYSMTEVVLIISAIKRADLQSKGIESPSISQAGILKELVA
                     KIVG"
     gene            7949..11008
                     /locus_tag="VGA21_00380"
     CDS             7949..11008
                     /locus_tag="VGA21_00380"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9648986.1"
                     /translation="MVKGLTRLILTLLFFQTTISSWSQSSSPLNNSEFYFNQGVELID
                     KANFSAARSNFEKYLNQNTNEFLNADAEYYIAFCAINLFQDDAEKLYDNFIKKHPTHP
                     SAESAYLELANFYYRGKNYQKANEYYQKVNFSRLTTSRWQEANFHAGYSNFATRNFAE
                     ALPYFNAIKRQSGKYSAASNYYAGYAEFQVEDFTSAVEDLERAEKEPAYKIQVPHMLI
                     SSYYKLARYEKAISYGEKVLEENDRLAQSSEIYLVTGDSHFALKQYPEASEYYELYQE
                     LAGSQASPVIQFKIGFAHLSAGKDQMAEEALKKVAIQNDDAGYLASYYLGTIYAKQKN
                     FEFAITSFEKSKRYSEDKTIAEESLFNLAKINFESDKPGQAVTHFNEFLKAYPNSNHK
                     REANELLSRSLLNSSNYDLAILHIETLPSIGSEVARVYQKATFLKGVELFNKRNFPAA
                     VEYWSKSLKHPFDNELVVDAHLWKGEAYSIGRRFDEAIESYLTVLGNRSVGESTILKA
                     RYGIGYAYFNTKDYDRALVHFREYVRAGSGLVNSSNFQDAQLRLADCYYVAKSYSEAL
                     TYYRRISESGPEKAYAFLQIGIIQSLQNNMPAAKASFEKVINDYSRSRYLDDAMFERA
                     QIDFERGFNQEAIVWFDKLVNNNPQSKFTPFAIQRRAIANYNLKEYNKTIEDYVFFLK
                     EHPGHKEVNNVLLGLQDALAIQNRTSEFQNYLDEFKDNNPTQKGLEAVEYQAAKNLYF
                     AQDYERAIPGFKAFIDNYPDDINVIEARYYLAESYYRSEKNEDALKIYSELLNDRTFS
                     EISRVIQRIAEIEQKEKRLENAIHFYHQLLTVASNKRQQYVAWSGLMESHFAAYNYDS
                     VQYYAQTILDRGAASPDGINKATLYQGKASYSMGDFERAKDSFINTLNTAKDEYGAEA
                     QYLLAEILHLEEQFKQSNEALFDLHKTFSSYQLWYDKSFLLIADNFVKMSETFQAAQT
                     LQSLIDNSTLSFIVDQAKLKLAAITKAPQLDSIKTDTTRERREGKNE"
     gene            11001..12683
                     /locus_tag="VGA21_00385"
     CDS             11001..12683
                     /locus_tag="VGA21_00385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9648987.1"
                     /translation="MNNIVNHYLIKLVLTCCFSILYISASLTFGQTWEEQPGEIEEAE
                     VVIEKNIKIVLPFAARNFEKIPPQPVSIRPGALTYSFASFLPVIQPINPRLRVVTISD
                     EEISKLYGGYLKAGFGNYTSPLLNAYIFTKRNEKFSLGAFGNYLSSFRGPVDKKNSAS
                     SDLNLGVTGSIHGEDVFLRSEIDYSRLGRYFYGYQDGLLVDRDTIQQIINRFNVRLNL
                     SGELSTTPWLYNLGAGYRYIGDEYDATENEARISGGLGYKLDADGKIVLDAEYMNLGR
                     SDNLIDGLNRNLLQIAPAYHFTYNKIQILAGINMTVENDTIKGLNKFHLYPDINATFN
                     ISGSLSLYGGINGKINVRSLDYWLQENPFLNENTPIFHSNQNLEVYGGLYGQLVNPLG
                     FQLGASIASVKNMSYLVNDSQDISRFTLVYDSMKTSIANVFGQLSLQKEQARIWFRGD
                     YWGYNTGAVQEAWHLPSFEITAGTKINIYEKIMVSGNFKSLFGIKAYDPVSQLSIDLD
                     PVLKLDIEVEYLLSKIAGIFVQANNVISSPNEAYFRYPSRGFQVIGGLTFSF"
     gene            12839..13594
                     /locus_tag="VGA21_00390"
     CDS             12839..13594
                     /locus_tag="VGA21_00390"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEX9648988.1"
                     /translation="MAKKRNDDDDINEEFGQDSSDDINEADDNFGLPDVEYKTLDESD
                     EPKPESEGSESEKKSQVYSQTSYSSEERASSNSGYEPPKAESIAPKLILFAVLLIIAA
                     GAIWYFGFYSPAQKAEQARIEQQRQAAEAEEAARRAEQQRLARERAVQDSLARVAAAQ
                     AEVPEVGIMTTISGRTGRYYVVVGSFVDDDLAKDLANKLGQDGINTSLIPPWGTRKFY
                     RLAMSDLESFDEAQARADELKPTYGEGLWVLKY"
     gene            13594..14277
                     /locus_tag="VGA21_00395"
     CDS             13594..14277
                     /locus_tag="VGA21_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009580788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEX9648989.1"
                     /translation="MILLQISTTSPDSLNTGTGDATMSIFDLLIKGGYMMYPIILLSI
                     IAVYIFVERVLTLRRAAETPPGFIDKIKGLVLSGDVKGAQQSCAQTDTPISKMIEKGL
                     ARIGSPLKNIEVSIENVGKIEIYKLEKNLGLLATISGAAPMLGFLGTVLGMIQAFIAI
                     AQEEGSVSPKLLSEGIYEAMITTAAGLFVGILAYLGYNYLVTRVQKVVHSMEFSSIDF
                     IDLLQKPKS"
     gene            14284..14673
                     /locus_tag="VGA21_00400"
     CDS             14284..14673
                     /locus_tag="VGA21_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018475889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEX9648990.1"
                     /translation="MDLKSRHRVDAAFSMSSMTDIIFLLLIFFMLTASFVTPSGLPVN
                     LPTSKSTTIVMQKVSVSITNDLQYFVNNRRVDKADLETALARELNGQEGVVSLHVDKN
                     VTTEHLIYVAGIATSLKARVSVVAKPN"
     gene            14675..>15315
                     /locus_tag="VGA21_00405"
     CDS             14675..>15315
                     /locus_tag="VGA21_00405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9648991.1"
                     /translation="MSELEEKKNRKIGWSVSIGVHLAVFLLFLFLLAWREPNPPYPEY
                     GIEINFGLDDAGTGNIQPENEAADEEETKEPSIEETTKQVEDQPEMTGQSEEIPEVIE
                     NEDAPEIVEEKPVEKPVTTPVVKEEAKKEETKPVEKPATVAETTPKKETTQQTETSES
                     TSQGDNASKTGDKGDPQGDLDSRALYGNKGGGDGGNSLELTGWDWGDKPKEDP"
BASE COUNT         4777 a         2974 c         3270 g         4294 t
ORIGIN      
        1 cttggtaaaa cggaacctaa tgcataatta agatccaatc ctaaggggaa gttttcgatt
       61 cttcctgtaa tagtaacacc gttatccaca aagccgctta agacaatgtc caatgatcca
      121 acaccttcaa ccgtaacagt cagggttagt tgtgtacggc ctgcatcaat tacccatgcc
      181 ccctgctgag cttccgcggc atctccaccc gcgcaaacat tccaaagagt tccatcagca
      241 cgaagttcag tccttgcgtt tgtcgcggtt gcacaaggtg agctggcata caagccctgg
      301 ccaataataa gtgtggtagt agtttgatca tttgcggcaa ttgttccaat tgaggtagcc
      361 gatggaaata cgacattcgt ggtattgatc atcgttccca atgtaaatgc ataagtaccc
      421 accaaagggt ctgtatcatc atcttccgaa caagaggtaa atagcatgct tgtgcttaac
      481 atgactccca taaggaggaa tgagtataat ttcattagct tcatagttcc attattttag
      541 gtaaacatca aatttaggtc taatttacct gataccaaac ttaagcagtg aattttgtgc
      601 ctaattaatg ccctttgtaa agacaggtaa ttcccttttt atttgtggaa taattgaatt
      661 gtcaaaaaaa ccttaaaatg agtattattg catattctaa ctaatggaaa ttatctgctt
      721 gcccgaatat aagcgttcga ttttaatgat tctattcctg gcggtgtttg ccatagttac
      781 acatgcccag actactatta ctggaacaat aaaagatgag aatggagaac ccttgatcgg
      841 cgtgaatatt attatgaaag ataaacttac aggtaccgtt acggatacaa agggaacctt
      901 tttcctgcag acagaatcaa atcctccctt taccctcgtc ttctcttatg ttggttatga
      961 aacaaatgaa gtggatatca gaaaccagac catcattgac ctgaacctaa aagaacaggt
     1021 aatgctcgga caagaggtag ttatatccgc ttcaagggtg gaagagaata tcctccaatc
     1081 gccggttaca attgaaagga ttggaatcct ggaaatcagg gaaaatgccg ccacagattt
     1141 ctatgatcgt ctcgggagtc ttaaaggagt tgacatgaat aagcaggggt tcacattcaa
     1201 agtacccaac acacgcgggt tcaacggcaa caccaactat agagtcaatc aaattgttga
     1261 tggaattgat aacgctcctc cgggattaaa ttttgctgcc ggaaacattt ttggactaag
     1321 ccagttggat gttgaaagtc tggaattgct ggttggtgct tcctctgcac tatatggacc
     1381 tggcggaatg aacgggacac tccttatgac cagcaagaat ccttttgaat accagggtat
     1441 cagcgcgtat gtgcaaactg gaattcatca tcttgccgct gactatagaa acacgccaac
     1501 tccaatggta gatgccaatc taagatgggc aaaagcctgg aaaaataagt ttgccatgaa
     1561 ggtggtggct tcttacatcc aggccctgga ttggcatgct gatgattacc gggatcgcaa
     1621 tgaccttgat gatccaaatc tgacgcgcga aaccaatgca ggttatgatg gggtaaatgt
     1681 ttatggagat gaagcaattg ttccggttaa tctgcaggat ttggctccaa cagttgggga
     1741 tggagtggcc aggcagcagg ggtatcaacc cggcgatcct gaatacgatg caattttaca
     1801 gctgatcgaa gatttgtttc cggaccagat tattacacga accggctata aagaaagaga
     1861 cctggtagat tacaatacaa gcaatttccg gatacaaact tctatgcact atcgattaag
     1921 tgaaaaagtt gaagcaatat ttgccggcgg ctatggcaat ggaacttctg tttatactgc
     1981 tcaaaataga ttttcactac gtgattttga gatttaccag gggaagttgg aattgaattc
     2041 taataatttc tttgtcaggg cctggggggt taaagaatct tctggaaaaa cctatgatgc
     2101 cggagccact gcattgctta ttaatgaggc atggaaacct tcccaggatt ggtataccga
     2161 attcatccaa acttttacta ccaacctttt gttgggaaac cctgtagcca gcagccttgc
     2221 aactgcccgc acggtagcag acaatcgcac acctcaggga gggatattaa atccgggatt
     2281 acccacaatg ccattacccg gaactccgga gtttgaaagt tactttaatg atattacttc
     2341 acgaaccgta gatcagcagg gttccggaat aattgataaa acttccatgg taaatctcga
     2401 atttatgtat gactttacag gaaagattgt gtttgcggat atcctggtag gagcaagcga
     2461 cagggtttat aatgtaaatt ccgagggaac catcttcttc gatgaaccca acgatccaat
     2521 tattcaaaac cagtttggcg cttatgccca aatatctaaa aaactaatgg ataagaaatt
     2581 aaagttcacc ggttcggtaa gatatgataa aaacgataaa tacgaaggta gaattactcc
     2641 cagaggctct ttcgtttact cttttggaac cgataggcga aataatttca ggggttcggt
     2701 gcaaactgct ttccggtttg cttccatttc agatcaatgg gttgatctaa atgttggtcc
     2761 ttaccaggta attggcggac tgccggaagt gcaggcaaag tatgatttcc atataaaaga
     2821 cgtctacccg ctgataggat caaataccct agatagccag cccgatctga gtaacggacc
     2881 tttcgtaatt ccggaatttg gcccggaacg cgttgttgct tatgaaattg gctacagagg
     2941 tttattcttt gataatcgta tgctggtgga cgcctatgga tactataaca gatttaacgg
     3001 tttcctggca actcaaaacc ttgttatgga tcccaatact cccgatgaaa gaagatttca
     3061 gacaacggta agtactgatg atcctgtgag tgcctatggc tgggcattag gaattgatta
     3121 ccggtttaac cggggctatt tgctgcgcgg aaatgtagct cacaacaccc ttgaacctct
     3181 tggtgacaga cctcctggct ttgaatcacg attcaattcc cctaattaca gaacaaatat
     3241 tactgtttca aatctgcatg tcataaataa cattggattc agtattaact ggcggtggca
     3301 aaatagattc ctatgggagt caacgtttgg ggtgggagag gttccggcat ttcataccct
     3361 ggatgcccag gtaagtttta agctgaacgc actaaaatcc gtagttaaag ttggcggtgc
     3421 taacctgcta aaccaatatt atactactgg ttttggaaac cctgccatcg gaggtttata
     3481 ctatgttacc ttaacttttg accagttcat gaattaaaac aaatattaga acgatatgag
     3541 aagagtaatt ttattaccaa ttgttcttta cattttaaca gcttgtactc cggcagaacc
     3601 tcagattctg caggctgaag ttgaaagaat cattggaacc cttgcctctg atgaaatgaa
     3661 ggggcgaaga actttttccc ctgaaattga tctggcatcg gattttattt caggtgaatt
     3721 tgaaagaatt ggattgcaga aatgggatgg gttggattcc tataaacaag agttctacgt
     3781 cttcaatttc acggttgatt ccctggttgt taagacagat ggaaaaagcc tgggtatggc
     3841 atccgctact acaacgagtg aaaatatttc ctggacagaa gaaagcaatg ttgccagcgt
     3901 ttttgtgggt gaaaaagaca cattgcaaag gacttacggc aggtatagga gagccggtga
     3961 gaacgtaatt ctgtggatac atcctgccca tcaacaactt tacacccgat acgcatcctt
     4021 tcttgcaacc ggttccagga caatgacaag agcgggaggc tcaaccattg tttttgctat
     4081 tagtgaatct aaacctgaac tgttttctgt ggagttcacc gcatcggtaa ctgaactgcc
     4141 gctggctaat gtggttgggt atattgaagg caaccggaaa gatgagtatg ttttgttctc
     4201 agcgcattac gatcatattg gtattcaaag gcctgtggaa ggagactcaa ttgccaacgg
     4261 tgcgaacgat gatgcttccg ggagtacggc ggttattatg cttgctgatt attttaaggc
     4321 taaatccaag ccggaaagat cgttgttatt tgtaaccttt accgccgagg agattggagg
     4381 ctatggatca cgttactttt cacaacaatt ggatcccgac aagattgttg ccatgtttaa
     4441 tattgaaatg attgggaagc cggctacaga tggcccgaat tcagcctgga taaccggttt
     4501 tgataaatct gactttggac agattcttca aaagtcagtg gaagggaccg agtacaaatt
     4561 ctatcctgac ccttacccaa ctgaaaactt attttaccgc tctgacaatg cgacactggc
     4621 ccggcttggt gtgcccgctc attctattag tacgactcca attgacgtgg acccggatta
     4681 ccatcaggtt tcggatgagg taaaaacctt aaaccttgag catcttgcca acacaataaa
     4741 agcaatctct gctggtgcgg caactattat ttcaggcgaa gcaactccca ccagagtaaa
     4801 tccggcgaat gtaaattgac cgttgaaaat aattcctaac aatgagttga atcgtgatgc
     4861 ctgggattta ctcatcgcga cttcggagca ggggaatttc tacgctcttt cctggtacct
     4921 ggatattgtg tctcccggtt ggtcagcaat tgtagaaggt actcatcagg agtaccggat
     4981 tggaattcca ttacccgtta cccgaaaatg gggatttaga attcttaaac aacctttatt
     5041 ttgtcagcaa ttgggtattt actccatcac tcaaccaact caagacgttg tggctgaaat
     5101 agtgaattta atcaccagga aatttaatta cattattaag tattccttca gtaatttcaa
     5161 cactccgcga ttattgtccg ggagtttatc agaaaatttg aacgtggtac tgaaccttaa
     5221 ccagccctat gatgtattaa aaagtaatta taaaagagac agaaagtaca gactgatgca
     5281 ggtactgaat tctaatgttt ttacccagga aagcaatagc cttgaagagc tcatttgcat
     5341 gtttaaagaa aatggcgaaa agaaaatata tgggggtgtg tcatctactg catacgaaat
     5401 gctccatcag attttttatg aaaccgcaac cagaggatta gcgaatctta tctatgccaa
     5461 agttcctccg gataagatca ttgccggtgc tttgtttatc aaattcaaaa acagaataac
     5521 ctatatgttt aatgccggct attccgaatt tgtaaaatta aatggccgga cattgatttt
     5581 agattcagtt atcaggaagt atcaaaacca acccctgcta ttggattttg aatgccctgg
     5641 gcttaaaaat atcagcacct tctatcgaag ctttggcgga ctggatgaga tttacttgag
     5701 cctttctcaa aaccatttac cctggccggt gaaaaagatt catgcaatga ggaaaaaata
     5761 ttatgagcga gacttgtatg ataagtccgc gagaaacggt tcgtaatatc aaaaatgtgg
     5821 aatctttggc catgctctta aagtcattaa ttttccgata ccaccagggc aattttactc
     5881 atatctttat taacggcaag cctggttatt ccatctaaat taaaacttga aatatcagca
     5941 attaacttcc attcagtcat tcctttactc caacgaaata gtttcgaacc gctgcccata
     6001 ataagggttc cattttgcgt ccaggcgcaa tcctgagaat tctccacaga gcgtatcaaa
     6061 agttcggata ttccggtttt taaatcataa gatcttattt cagaattacc ggtatcatca
     6121 ttctgaatat agctaaaata atccgtacct gggattctgt gcagtgagcg gccaatagat
     6181 tcgccgataa tttcactttg tccggtggag acatcaatta agtgcattgt atttggatca
     6241 cccagaacaa aagcagcaat ctggtgatca cttacccagg catgataccc caccttcagg
     6301 ccggcagcaa gtaaaaccgg cttgttgccc ttaacatcaa agttatactg ccataataat
     6361 tgcgttccat ccggctctaa cagaatcgtg ctgaaatgtt cgctatccgg agttggcgtc
     6421 ggagaatact ctccgcctgg ggttgcggtg atttgctttg aacttccatt ggagtaatga
     6481 taaacatcag tctggccatt aatagaagat acgtaaagca aacttccatc ttctaaaaaa
     6541 tgcggttgat tgtcataacc cttgtggtta cttacattta caggattggc aatttgcatt
     6601 tccccttcac caaattgcaa atcaaaaata taaacttcag tggctggctg cgctaacgca
     6661 ccaatagaaa ggcagcaaaa aagatagcac attagatttt tcatatgata aacttaacaa
     6721 attatcagat tttccaccat gagaaaatat ataaatcctt tatctcgatt atttttgtta
     6781 attataaatt cttacaaaag ccttttcaaa tgaaaacggc cttcctaata ttgaaagatg
     6841 aatttgacgc ccaaagagat aatcaaacag ttaaaatccg gtaagtatga aagtatttac
     6901 ttccttatgg gagcggaatc tttttatata gatgaaattt ccaattttat tgaagaaagt
     6961 gccctggatg actctcaaaa agaatttaat caaacaatac tgtacgggat ggatgtaaag
     7021 atttcagata ttttaaccca tgcccggcga ttccccatga tgtcagagcg gcaagtgcta
     7081 attgtcaggg aggcccaatc tgtttcagat atcaataggg aaggtggaca aaaattgctg
     7141 ctggattata tatccaatcc gttgccaagt accattttgg tcttctgcca caagaacaaa
     7201 acacttgata aaagaaaaag tttatataag gcactaacca aatcatcttc agtagtcatg
     7261 gaatccgcaa tcataaagga atacaaaatt ccaggatgga taaaagagag aattgcagag
     7321 aagggtttga agatcaccga aaaggctgtt atgatgctta cccaaaatat cggctcaaac
     7381 cttgagagaa taaataatga aattgaaaaa gttagcatca atataaaaac taaaactatt
     7441 gatgataatg ccatccaaac ttatgtagga attaaccgtg actacaatat ttttgaattg
     7501 gtgaatgcgt taggagcaag gaacaaggtg aaagctcatg aaatagcttt ttacctggga
     7561 actcactcaa aagaaaatcc tttggtctta atcattggct ttgtattttc ctttttttcg
     7621 aagctgttaa aattatacgg agcgtccggc aaaaccgata aagatcttta ctcaatactt
     7681 ggtacttctt acattagtga ttacaaagtt gcagcaggta gctattccat gaccgaggtc
     7741 gtgttaatta ttagtgccat taaaagggcg gatttgcaaa gcaaaggaat tgaaagccca
     7801 tcaatttccc aagccgggat tttaaaggaa ctggttgcta aaattgtagg gtgaaagaat
     7861 tatgagagca ataagaatat tttttgaaaa ctttcgttaa tatgcctaaa gaagcaggca
     7921 gaaatttccc atttttgtgt gacataatat ggtcaaaggg ctcaccaggt taatactcac
     7981 attacttttc tttcaaacca cgatttcatc atggtctcag tcatcctcgc cacttaataa
     8041 ttctgaattt tactttaacc agggcgttga attgattgat aaagccaatt tcagtgctgc
     8101 aagaagtaat ttcgaaaaat atctcaatca gaatactaat gaattcctca atgctgatgc
     8161 agaatattac attgcttttt gtgcaataaa cctttttcag gatgatgcgg aaaaattgta
     8221 tgacaatttc ataaaaaaac accccactca tccaagtgct gaaagtgcgt atttggaatt
     8281 agcgaacttt tactaccggg gaaagaatta tcaaaaagca aacgaatatt atcagaaagt
     8341 aaatttttcc agactgacca cctcgcggtg gcaagaagcc aattttcatg ccggttattc
     8401 aaattttgca acccgcaatt ttgctgaagc tctaccttac tttaatgcca ttaagcgcca
     8461 gtcaggaaaa tactccgccg cgtcaaatta ttatgcgggc tatgctgaat ttcaggttga
     8521 agattttact tccgctgtcg aagatctgga aagggcggaa aaggaacctg catataaaat
     8581 tcaggtccct catatgttaa taagttctta ttacaaactt gcccggtacg agaaggctat
     8641 ttcttatggt gaaaaagttt tagaagaaaa cgacaggctt gctcaatcat ccgaaattta
     8701 cctggttacc ggagattccc actttgccct taaacaatac ccggaagcat cagaatatta
     8761 tgaactttat caagaattgg cgggaagcca ggcctcgccc gttatccagt ttaagattgg
     8821 gtttgcccac cttagtgcag gtaaagatca aatggccgag gaagcattga aaaaggttgc
     8881 catccaaaat gatgatgccg gatatcttgc ctcctattat cttggtacaa tttatgcgaa
     8941 acaaaagaat tttgaatttg ccatcacatc atttgaaaag tcaaaaaggt attctgaaga
     9001 taaaaccatc gcggaagaat ctctatttaa ccttgccaaa atcaattttg agtcggataa
     9061 gcctggtcag gctgttactc attttaatga atttttgaaa gcctatccaa acagtaatca
     9121 taaacgggaa gccaacgaac ttctgtccag atcactttta aattccagta attatgattt
     9181 agccatttta catattgaaa ctctcccatc aataggcagt gaggttgccc gggtgtatca
     9241 gaaagcaacc ttcctcaaag gtgtcgaatt gttcaacaaa agaaattttc ccgccgcagt
     9301 tgagtattgg agcaaatcgc ttaagcatcc atttgataat gaactcgtag tcgacgcaca
     9361 cctttggaaa ggtgaagcat attccatcgg gagaagattt gatgaagcta tagaatccta
     9421 cctgaccgtt ctcggtaata gaagtgtagg tgaaagcacg atattaaaag cccgctatgg
     9481 aattgggtat gcatacttta atacaaaaga ttatgaccgt gctttggttc atttccgcga
     9541 atatgttcgg gcaggttccg ggttagtaaa ctcgagcaat ttccaggatg cacagctaag
     9601 acttgcagat tgttactacg tggccaaatc ctattctgaa gcattaacct attaccggcg
     9661 aattagtgag tctggccccg agaaagcata tgctttcctc cagataggta ttatccagtc
     9721 gttacaaaac aatatgccgg ccgcaaaagc atcctttgag aaagtaatca atgattattc
     9781 cagatcaaga tatctcgatg atgctatgtt tgaaagggcg caaattgatt ttgaacgagg
     9841 tttcaaccag gaagcaatag tttggtttga caaactggta aacaataatc ctcaaagtaa
     9901 atttacaccc tttgcaattc aacgcagggc tattgcgaat tataacctga aagagtataa
     9961 caagacaata gaggattatg tttttttcct gaaggaacac cctggccata aagaagtaaa
    10021 taacgtgttg cttggtttgc aggatgccct ggcaattcaa aacagaactt cagaatttca
    10081 gaactacctg gatgagttca aggataataa tccaacacag aaaggtttgg aagctgtgga
    10141 ataccaggca gcaaaaaatc tttacttcgc ccaggattat gaaagagcaa ttccaggatt
    10201 caaagcattt attgacaatt atccggatga tattaatgtg attgaagctc gttattatct
    10261 cgcggaatct tattaccggt cagaaaagaa cgaagatgcg cttaagatct attcggaact
    10321 gttgaacgac agaacttttt cagagatcag cagagttatt caaagaatag cggaaattga
    10381 gcagaaggaa aaaagacttg aaaatgcaat acatttctat catcaactgc ttacagttgc
    10441 atcaaataaa cgacaacaat atgtggcttg gtccggattg atggagtctc attttgctgc
    10501 ttacaattat gactccgttc aatattatgc acaaaccata ttggataggg gcgccgcaag
    10561 tcctgatggc atcaataaag caactttata ccagggaaaa gcttcttatt caatgggaga
    10621 ttttgaacgc gccaaggata gcttcattaa caccctcaat acggccaagg atgaatatgg
    10681 agcggaggct cagtatttgc tggccgaaat tctccatctt gaggagcaat ttaagcaatc
    10741 caatgaagcc ctgtttgatt tgcataaaac cttcagcagc taccagctct ggtacgataa
    10801 gtccttcctg ctaattgctg ataattttgt taaaatgagt gaaacatttc aggctgccca
    10861 aacactgcaa tccctgatag ataattcaac ccttagcttt attgttgatc aggctaagtt
    10921 aaaactggca gcgataacca aggcgccaca acttgacagc ataaaaacgg atacaacccg
    10981 ggaaagaagg gagggaaaga atgaataata tagtgaatca ttacttaatt aaattggtac
    11041 taacttgttg cttctcaata ttatatattt cggctagttt aacatttggg cagacttggg
    11101 aggagcagcc tggtgaaatt gaagaagcgg aagtggtgat tgaaaagaat atcaaaattg
    11161 tattaccttt cgcggccaga aattttgaaa aaatcccacc ccaacctgta tccatcaggc
    11221 ctggtgcatt aacttattcg ttcgcatcct ttctgcctgt tattcaacct attaatccca
    11281 ggctaagggt ggtaactata agtgatgaag aaataagcaa gctatatggt ggatatttga
    11341 aagcagggtt tggtaattat acatcgccct tgctgaatgc atatatcttc accaaaagaa
    11401 atgaaaaatt cagcctgggt gcatttggca attacctgag ttccttcagg ggccctgttg
    11461 acaaaaaaaa ctctgccagt tctgatttga atcttggcgt taccggaagt attcatggag
    11521 aagatgtttt tctacgatct gaaattgact attcaagact tggaagatat ttctatggat
    11581 atcaggatgg tttgcttgtg gaccgcgata caatacagca aataataaac aggtttaatg
    11641 tcagattaaa tttgtcaggc gaactatcaa ctacaccatg gttatacaat cttggagcag
    11701 gttaccggta tattggcgat gaatatgatg caacagaaaa tgaagccaga attagtggcg
    11761 gcctgggtta caaactggat gcggacggca aaattgttct ggatgcggaa tatatgaatt
    11821 tgggcagaag cgataatttg atagatggct tgaacaggaa tttactgcag attgctcccg
    11881 cctatcattt tacctataac aagatccaga ttctggcagg aattaatatg actgttgaaa
    11941 atgacacgat taagggactg aataaatttc acctgtatcc tgatatcaat gctacattta
    12001 atattagcgg cagcctgagt ttatatggtg gcataaatgg taaaataaac gttcgcagcc
    12061 tggattattg gttgcaggag aatcccttcc tgaacgagaa tacccccatt tttcatagta
    12121 accaaaatct ggaggtttac ggaggccttt acggtcagct tgtgaatccc cttggattcc
    12181 agcttggagc ttccatcgcc tcggtgaaaa atatgtctta cctggtgaat gattctcagg
    12241 atatctcaag attcactttg gtttacgatt cgatgaaaac gtcaattgcg aatgtatttg
    12301 gtcaacttag ccttcaaaag gaacaagcaa gaatctggtt tagaggagac tattggggtt
    12361 ataataccgg tgcagtccag gaagcgtggc atttgccatc ttttgagatt acagccggca
    12421 caaagattaa catttatgag aagattatgg tttcaggaaa ttttaagtct ttatttggaa
    12481 ttaaggctta tgatcctgta agtcaattat ctatagatct tgatccggtt ctgaaacttg
    12541 acattgaagt tgaatattta ctttcaaaaa tagcgggtat attcgttcag gcgaataatg
    12601 ttatttcgtc cccaaatgaa gcttatttca ggtatccatc ccgtggcttc caggtaattg
    12661 gaggattaac cttctcgttt tagcctttcc gggctgttcc ggaactgaaa tatcttcaag
    12721 gcaacccttc cattgccatt tattacagaa aattcttaat tttttttaat aattgggttt
    12781 ttttaaggtt taatatttat ttctaacttt aggaaattaa aataataacg ataagtccat
    12841 ggcaaagaaa agaaatgacg atgatgatat aaatgaagaa ttcggacaag attcttccga
    12901 cgatatcaat gaagcggatg ataattttgg attgccggat gtagaatata aaactttgga
    12961 tgaatcagat gaaccaaagc cggaatcgga gggttcagaa tctgaaaaga aaagccaggt
    13021 ttatagtcag acatcttact cttccgaaga gagagcatca tccaattcgg gttatgaacc
    13081 acctaaagct gaatcaattg cacccaagct aattctgttt gccgtgttgc tgattattgc
    13141 agcgggtgcc atctggtatt ttggatttta ttcacctgca caaaaagccg aacaagccag
    13201 aattgaacag caaagacaag cggcagaagc tgaggaagct gccagaagag cggagcagca
    13261 aaggctggca agagaaaggg cagtacagga cagtttagcc agggtagcgg ccgcccaggc
    13321 tgaggttccg gaagttggta tcatgaccac aatttccggc cggaccgggc gttattatgt
    13381 tgtagtgggc agctttgttg atgatgacct tgccaaagac cttgcgaaca aattgggcca
    13441 ggacggtata aatacatctc taattcctcc ctggggtact agaaaattct accggcttgc
    13501 aatgtcagat ttggaaagtt ttgacgaagc acaggccagg gcggatgaac taaaaccaac
    13561 atatggagaa ggcctttggg tattaaagta ttgatgattt tactgcagat ttcaacaact
    13621 tctcctgatt cgttgaatac gggtaccgga gatgctacca tgtcaatttt tgatttgctg
    13681 attaaaggag ggtatatgat gtatcccatc atacttcttt ccatcatagc cgtatacatt
    13741 tttgttgaac gcgtactcac attgcgcagg gctgctgaaa cccctcctgg atttatcgac
    13801 aaaataaagg gcctggtttt aagcggagat gtgaaaggag ctcaacaaag ttgcgcgcaa
    13861 acagataccc cgatttccaa aatgattgaa aaaggtctgg cccgcatagg cagtccgcta
    13921 aagaatattg aagttagtat tgaaaatgta gggaaaattg aaatctataa attggagaaa
    13981 aaccttgggc tattagccac aatatcaggg gccgctccaa tgcttggatt tttgggcact
    14041 gtcctcggaa tgatccaggc atttattgca atcgcgcagg aagaaggttc agtaagtcca
    14101 aagttgcttt cggaagggat ctatgaggct atgattacta ccgctgccgg cttatttgtt
    14161 ggaatacttg cttacctggg ttataactac ctggttaccc gggtgcagaa ggtagttcac
    14221 agcatggaat tttcctccat tgatttcatt gatctgcttc aaaaacccaa aagttaaaat
    14281 aaaatggatc tgaaatccag acatagagtt gatgcagcct ttagcatgtc ttccatgaca
    14341 gacataatct ttcttctgtt gatctttttt atgctgacgg catcctttgt aactccgtcc
    14401 gggttgcctg taaatcttcc aacaagcaaa agcaccacca tagtgatgca gaaagtttcg
    14461 gtttccatca ccaatgattt gcaatatttc gtaaacaaca gaagggtaga taaagctgat
    14521 ttggaaacag ctttggcaag agaattgaat ggtcaggaag gagtagtatc attgcatgtg
    14581 gataaaaatg tcactacaga acatttaata tatgtcgcgg gaattgctac atcattaaaa
    14641 gccagggttt ccgtggtcgc aaagccaaat taagatgtct gaattagagg aaaagaagaa
    14701 caggaaaatt ggatggtcag tctccatcgg cgttcatttg gccgtatttc tgctattttt
    14761 gtttctgctt gcctggaggg aacccaaccc gccttatcca gaatatggta ttgaaataaa
    14821 ttttggcctg gatgatgccg gcacaggaaa tattcaacct gaaaatgaag ctgctgatga
    14881 ggaagaaacc aaagaacctt ccatcgagga aaccacgaaa caggtagaag accaacctga
    14941 aatgaccggg caatccgaag agattccgga agtaattgaa aatgaagatg ctcctgagat
    15001 agtagaggag aagcctgttg aaaaaccggt aaccacaccg gttgttaagg aagaggcgaa
    15061 aaaggaagaa acgaagcccg ttgaaaagcc cgccacagtt gcggaaacta ctcccaagaa
    15121 ggaaacaaca cagcaaacag aaacttcaga gtcaaccagc cagggagata atgcaagcaa
    15181 aaccggtgac aagggtgacc cgcagggtga tctggattca agggctttgt atggaaataa
    15241 gggcggtggt gatggaggca acagccttga attaaccggt tgggattggg gtgacaaacc
    15301 taaagaagat ccgta
//
LOCUS       DASWHX010000009         4105 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_3331318, whole genome shotgun sequence.
ACCESSION   DASWHX010000009 DASWHX010000000
VERSION     DASWHX010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 4105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4105
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3331318"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1176)
                     /locus_tag="VGA21_00410"
     CDS             complement(<1..1176)
                     /locus_tag="VGA21_00410"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX9648992.1"
                     /translation="MKKKVKHETLLMHYAEKRSDYKGAVVPPIFQNSLFTFEDWDSID
                     HAFDDRAKSFIYTRGKNPTVNIVQEKLAALAGGQAAQLFPSGMAAISAAILHFLSQGD
                     HMVAIRNIYGPANNLIQKYLVPKMGIQVTLVDGDDAKDFSDAIQPNTKLFYLESPSTA
                     VFKLQDLKAISKLAKSKKIKTIIDNTWATPVFQKPLAMGIDMEMHSISKYISGHSDVV
                     AGLLIGSKKDLDSIFKREYEWLGAKIAPFEAWLILRSLRTLPIRLQMHQESARKVAKF
                     LESHPKVKLVRYPGLKSFPQYKLGRKQMSGYTGLMSFQLKTEKLPQIKKFVNALEIFS
                     IGVSWGGHESLVYAPAISYLKELDPARFKQLGISLGDIRISVGLENADDLMEDLNQAL
                     "
     gene            complement(1205..2644)
                     /locus_tag="VGA21_00415"
     CDS             complement(1205..2644)
                     /locus_tag="VGA21_00415"
                     /inference="COORDINATES: protein motif:HMM:NF015515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HEX9648993.1"
                     /translation="MERSQPKVKFIHILLPVLFLAFLIVYGMMIRPLFLDQPGFPLEV
                     VFLLAAVFSIAELLFIGFQWEEIQNAFVQKFIKGLTPLLILFAIGVIIGTWIVSGTIP
                     MLIFYGIKMINPTYIYFLAFFIPIIFSTLTGTSWGSVGTIGMVLIGIAAAIEADLGIT
                     AGAIIGGAYFGDKLSPLSDTTNMASMAVEVDLYEHIHSMLYTTLPSALIAGSLFLILG
                     FVYPPLSLEIESGSVTSTLNSISSIFGFSVFLLIPPIIVLYGSIRRKATLPTLLTSSI
                     AACLLALIFQRFTIGDVMVSLHTGFNVSMASWVPVIPENISQLFNRGGLFELNEVIFF
                     TIIVFIFVGSIDLIEAMPKIVDKVFSFAKSRPLVIISSLFATAFTNAITSNQNATSFI
                     IGDAFRKRYEQFKIPRKVLSRSIEDYGTMVESIIPWSATTLFMVGTLGVPFSQYWHWQ
                     LLSLINLVVAVILAVTGKGCFYNKNKNSY"
     gene            2831..3070
                     /locus_tag="VGA21_00420"
     CDS             2831..3070
                     /locus_tag="VGA21_00420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9648994.1"
                     /translation="MLNTYTQIYIQIVFAVHTHLLVGLKSSIAISDLKGKYNWQEGFG
                     AFSYSGSQIDTVIKFILNQEEHHKKKPSERNILIS"
     gene            3619..3897
                     /locus_tag="VGA21_00425"
     CDS             3619..3897
                     /locus_tag="VGA21_00425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9648995.1"
                     /translation="MEIMRHRIESYSTFSENDRTISHCNGSSYLNHAITHGNYAVSHR
                     KHGKFNENDRTISHCDGSSYPNYAITHGNYAVSHRKHGKFNENHATIS"
BASE COUNT         1310 a          840 c          817 g         1138 t
ORIGIN      
        1 taatgcctgg tttaaatctt ccatcagatc atccgcattt tccaggccta cggagatacg
       61 aatatctccc agggaaattc ccaactgttt aaaccttgcg ggatccaatt ccttgagata
      121 gctgatagcc ggtgcatata caaggctttc atgtccaccc cagctcactc caattgaaaa
      181 aatctctaat gcatttacaa acttcttgat ttgcggaagt ttttcagtct tcagctgaaa
      241 tgacattaac cctgtgtagc ccgacatttg tttccgtccc aatttatatt gcgggaatga
      301 tttcaacccc ggatacctga cgagttttac tttaggatgt gattcgagga attttgccac
      361 cttacgcgcg ctttcctgat gcatttgcag ccggattggc aatgttctca aactgcgcag
      421 aataagccac gcttcaaatg gagcgatttt agcacccagc cattcatatt cccgcttaaa
      481 aattgaatcc aggtcctttt tgcttccaat taataaaccc gccacaacat cgctatggcc
      541 actgatatat ttgcttatcg aatgcatttc catatcaatt cccatagcaa ggggtttttg
      601 aaaaacggga gtcgcccagg tgttatcaat aattgttttg attttttttg attttgcaag
      661 tttagatatt gctttaagat cctgaagctt aaatactgct gttgagggac tttcaagata
      721 aaatagtttt gtatttggct gaatggcatc gctgaaatct tttgcatcat ctccatccac
      781 cagggttacc tgaataccca tttttggcac caggtatttt tgaataagat tattggctgg
      841 gccgtaaata ttcctgatgg ccaccatgtg gtcaccctga cttaaaaaat gcagaatagc
      901 agcactaatg gcggccatgc cggatggaaa aagttgagcg gcctgacctc cggccagggc
      961 tgctaatttc tcctggacaa tgttaaccgt gggattcttg ccccttgtat aaataaaact
     1021 cttcgcgcgg tcatcaaatg catggtcaat gctgtcccaa tcttcaaatg tgaataaaga
     1081 attttgaaag ataggtggaa ccaccgcccc tttataatcg gatcgctttt cagcatagtg
     1141 cattaataga gtttcatgtt taactttctt cttcatcaat aagatgcttt atttcaatat
     1201 attatcaata tgagttttta ttcttgttat agaagcagcc cttgccggta actgccagaa
     1261 taacggctac cactaaatta attaaagaaa gcaattgcca gtgccagtac tgggaaaatg
     1321 gaactccaag cgtacctacc ataaataaag tggtggctga ccacggtatg atactctcca
     1381 ccatagtacc ataatcttct attgaccgtg aaagcacttt tctggggatt ttaaattgct
     1441 cataacgctt ccggaatgca tcaccgatga taaaacttgt tgcattctgg ttggaggtga
     1501 tggcattcgt gaaggctgtt gcaaaaagag aagatataat taccagcggg cgggatttcg
     1561 caaaagaaaa aactttatcc acgattttag gcatagcctc aattaaatca atgctgccta
     1621 caaaaatgaa aacaatgatg gtgaaaaaaa taacttcgtt taattcgaag agaccgccgc
     1681 gattaaataa ctggctaatg ttttctggaa ttaccggcac ccaggaagcc atagatacat
     1741 taaacccggt atggagggag accattacat cccctatcgt aaatctttga aatattagtg
     1801 caagcaaaca ggctgcaatg gaagaagtaa gcaatgttgg taaagtagct ttcctgcgaa
     1861 tggaaccata taagacaatg atgggcggaa ttagtaaaaa tacgctaaaa ccaaaaatgg
     1921 aacttataga attcagtgtg gatgtaaccg aaccggattc tatttccaaa ctgaggggtg
     1981 gatatacaaa tccaaggatt aggaaaagac tacccgcgat taatgcggaa ggaagagttg
     2041 tgtaaagcat cgagtggata tgttcataca agtccacttc cactgccatg gatgccatgt
     2101 ttgtagtatc ggagagtggg gaaagtttgt caccaaaata agcccctcct attatcgctc
     2161 ctgcagttat cccaaggtca gcttcaatgg ccgcggcaat tccaattaat accattccaa
     2221 tagttccaac cgatccccag ctggttccgg tgagcgtgga gaaaatgata ggaatgaaga
     2281 atgcaagaaa ataaatatag gttgggttga tcatttttat tccataaaaa attaacatgg
     2341 gaatagtgcc gctgacaatc caggtgccaa taattacgcc tatagcgaag agtatcagca
     2401 agggtgtaag tcccttaata aatttctgca caaatgcatt ttgaatttct tcccattgaa
     2461 agccgatgaa cagtaattcc gctatagaga aaactgcggc aaggagaaaa actacttcaa
     2521 gaggaaaacc gggttgatcc agaaacaaag ggcggatcat cattccataa acaatcagga
     2581 atgccagaaa aagtaccggc aagagtatat gaatgaattt gaccttgggt tgagaccgtt
     2641 ccatcaggag aaatttgggg catcaatata caatggaagg tgcaaatttt ctgtatgcgg
     2701 atggtaactt ctcagtcatt cctgaattca tccattccat ccctggctat tgttaatgtc
     2761 ccatagggac gatctgtttg tagaaaataa tgcaatgcaa taagtaagct ccgtaggagc
     2821 gacctgaaat atgctaaaca cctacacgca aatttatatt cagattgtct ttgcagtcca
     2881 cacacatctt cttgttggtt tgaaatcatc aattgcaatt tcagatttga aaggtaaata
     2941 taattggcag gaaggctttg gtgcgttctc ttattcagga agccagattg atacggtaat
     3001 caaatttatt ttgaatcagg aagaacatca taaaaagaaa ccttcggaga ggaatatatt
     3061 aatttcctga aaaaatttga aattgaatac catgaaaata tttgtttgaa tggatagaat
     3121 aaaacaggtc gctcctacgg agcttacggc tgttgaattc tgcgataact acaaacagaa
     3181 tgctcctaca gagcaatgat tagaaatgaa taaatgaaga agatagacaa actcattatc
     3241 aattctcctt acgccccgcc cttgatggtg aaatgaatac caaaggcgag gtttgaaatc
     3301 atttttcccc ttcagagtct ccgaagggac tctgaggaac taagaaaacc agtgcaatca
     3361 cccatacccg gtatgcgagc ttccatctaa aacatgcaat cgcccatacc agccaagcat
     3421 catcgcataa aaattactaa aattttcacc aaaaaaacac ggcatttagt atgtgactac
     3481 taaaattcct atccaaaaca tgtaatcacc catgaaaact atgcggcatc gcataaaaat
     3541 catgggaaat tcaatgaagc tgtactcaat atttagtacc tgactattgg atgtcctatc
     3601 caaaacatgc gatgacccat ggaaatcatg cgtcatcgaa tagagagcta ctcgacattt
     3661 agtgaaaatg atagaacaat tagtcattgc aatggaagct cctatctaaa tcatgcaatc
     3721 acccatggaa actatgcggt atcgcataga aagcatggga aattcaatga aaatgataga
     3781 acaattagtc attgcgatgg aagctcctat ccaaactatg caatcaccca tggaaactat
     3841 gcggtatcgc atagaaagca tgggaaattc aatgaaaacc atgcaacaat tagttagcgt
     3901 ggttcaatct cctgttaaaa acaggtaatt tggaggcggc acaaaaaaag ccggaacttt
     3961 cgccacggct tttccttagc gttaaatact ctattgcggg tcgttattac cttcggattc
     4021 ttccgaagga gcctcctcat tctttttgga atgcttaaaa cgcttggcca aatcttcatc
     4081 aatgggtttg gagcccgtta cattc
//
LOCUS       DASWHX010000010        23112 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_745302, whole genome shotgun sequence.
ACCESSION   DASWHX010000010 DASWHX010000000
VERSION     DASWHX010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 23112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23112
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_745302"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(501..1694)
                     /locus_tag="VGA21_00430"
     CDS             complement(501..1694)
                     /locus_tag="VGA21_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020529247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9648996.1"
                     /translation="MSRTPKTFNLQFWLLCLSSFLFFCSFNMIIPELPEYLTQLGGAE
                     YKGLIISLFTLTAGLSRPFSGKLTDKVGRIPVMVFGSLMAAVSSLMYPFLTGLAGFFI
                     LRLVHGLATGFKPTGTAAYIADIIPREKRGEALGILGVFLSLGMAAGPAIGSPIAIYL
                     SLNTMFYVSSVLGLLSVVVLFGMKESLPKTERFSLSLLKISPGEVLEPNVYFPALIMI
                     LSAFSFGMVLTIIPDFSTHLGLQNKGIFFTYFTLSSIAVRIFAGKASDKYGRRVVLLI
                     ALIMLLIAMILLGFTRTYFTFIIAAIFFGQASGMTSPTIFAWTIDLASDEHRGRAMST
                     LYIALEIGIGLGALYSGWAYGNNPANFAKTFWSGAMLALVAVIILIRFRHQKLPHVSR
                     TNENH"
     gene            complement(1723..2800)
                     /gene="prfB"
                     /locus_tag="VGA21_00435"
     CDS             complement(join(1723..2748,2750..2800))
                     /gene="prfB"
                     /locus_tag="VGA21_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139362050.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEX9648997.1"
                     /translation="MTNEQLKDLKERVTSLRGTFDYDNKKKEIASEEELTTQPGFWND
                     PKKGEAILREIKSKKVWTNSFEQLDKDFQDMLTLNEFYQEGEIQDSEMDAEYEKVLKA
                     LEDLEFKKMLSGEEDQLDAVVEINPGAGGTESQDWADMLLRMYIMWGEKSGYKVKQIY
                     YQPGETAGIKSAAIEVSGEFAYGYLKSEIGVHRLVRISPFDSGARRHTSFASVFVYPV
                     VDETINIEINPADITWDTFRAGGAGGQNVNKVETAVRLKHAPSGLIIECQQERSQHQN
                     KEKALNLLKSRLYQIEIDKKNAERDKVESSKKKIDFGSQIRNYVLHPYKLVKDNRTGI
                     ERSDPLNVLNGDLDDFIKAYLMQN"
     gene            complement(2845..3336)
                     /locus_tag="VGA21_00440"
     CDS             complement(2845..3336)
                     /locus_tag="VGA21_00440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9648998.1"
                     /translation="MKKILFVGIFLLLTEEAIAQKYLVLEKLGTRKRMEYYIGSEINY
                     RLNGEDFFRTGTIADLADSIIVFDYAFFPIENINAININDTRTRRISARGYPVTLIVA
                     GVVLFLGDLFNETVVSGNEASLNKGVTIASGALAGTGFLLMFVKSDIKKLTKNWRLRV
                     VDI"
     gene            complement(3402..4466)
                     /locus_tag="VGA21_00445"
     CDS             complement(3402..4466)
                     /locus_tag="VGA21_00445"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010608196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HEX9648999.1"
                     /translation="MIETLDLIITETKNSRISEIDFDNIQFAKIYSDHMFLADYKDGK
                     WQDFRISPYDYLRITPANATLHYGQAIFEGLKAYKSPGGDVLIFRPLDNFKRINISAE
                     RMCIPKVSEEIFMGGLTELLRLDKQWVPEKPGTSLYIRPFIFATDEYIGVKPSATFKF
                     MIITCPVGAYYATPVKVKIETKYTRAAQGGTGFAKTSGNYARSLYPAKLAQEKGYHQL
                     IWTDGRDHQYIEEAGTMNVMFVIGDTLVTPQLSESILAGVTRNSVLTLARDWGLKVEE
                     RKVSVKEVIDAARNGDLKEAFGAGTAATIAHIESIGYEEEDFGLPPLGKREFSIRLLD
                     ALNEIRYGKGEDKFNWVYKV"
     gene            complement(4534..5079)
                     /locus_tag="VGA21_00450"
     CDS             complement(4534..5079)
                     /locus_tag="VGA21_00450"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013453093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HEX9649000.1"
                     /translation="MKIYTKTGDAGETSLLGGSKVPKSHIRIHAYGTVDELNSHLGLV
                     GDQEVNKGRIESIKKIQNCLFVIGSHLAVEPGTKIQLPEISEQDVEYLEQLIDEMDKS
                     LPEMKNFVLPGGHVSVSIAHIARSVCRRAERWIIKLNSEEKVDPVIIKYVNRLSDFLF
                     VLSRKNANELIITETPWMPKK"
     gene            complement(5085..5804)
                     /locus_tag="VGA21_00455"
     CDS             complement(5085..5804)
                     /locus_tag="VGA21_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002698620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9649001.1"
                     /translation="MPQVINTKEISRIYQMGSETIRALNSITISIDKGEYVAFMGPSG
                     SGKSTLMNIIGCLDTPSSGTYILNGHDVSDLTENELAEIRNKEIGFVFQTFNLLPRAT
                     SLENVALPLIYAGYGKSDRNEKAMQTLTDVGLGDRAKHKPNELSGGQRQRVAIARALV
                     NDPSIILADEPTGNLDSKTSYDIMDLFQHLHDTGNTIIMVTHEDDIAHYAHRIVKLRD
                     GLVESDERNTDIRRPKAKMGV"
     gene            complement(5967..8966)
                     /locus_tag="VGA21_00460"
     CDS             complement(5967..8966)
                     /locus_tag="VGA21_00460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649002.1"
                     /translation="MQNIIFWNDWEKPYKIAINLLAVVFILSVSFLITGILWGESFVI
                     DWQVLNIKQPLAVTFDSFTKGFFNFNIVAENFVVFQKFQGSELQVNLPGSYIFGLFIA
                     ISIVFYLSAVTELSRFWYIVAMGVFIIFMVNLKLEHLQIFGRIDRIPLIIVFLILLPT
                     SYYFHAMNKESLYWKRLLVFAVLVTACCFGFYLFAGVEHPFLYLTNYGILGVLIITAA
                     FILMISHEIINAFIYLLTSSTTLVSRNTLRHFLVITAFYLGNIMMLYLHETRVIDWEF
                     YYINPYILLILSSTIGFWGLITRSEILKNAVPFYPWGPMLYLSVAIIATATTGYLFVT
                     GNDPVLEVIEDVIIYGHLGFGFFFFIYILANFGALFGKKTGIYKVIYKPTNMPYFTFR
                     FAGLIAVAALFLKENFEVPIYHSLSGYYNGIGDLYTATREYYLAEQYYKLGSDYGYQN
                     HRSNYALASLASKQGNDVTAIYFYEQAIKKRPTEYAYINVSNTLMANGQFLNGLFALR
                     DGLDQFPKSGPLMVNAGLAFRQTEILDSAAILFDQATAFKISERLGKNNLLAMVAENN
                     WQLDPDSLRESYYLKGNEISEINYSTLLNQNQKTGNYVPQFPSDSILGLVSSSQLINF
                     GMNSFIQGDDAFIAVMDSLSNKPENSRYSDEIQYILALGMLKNNRIDKSISILKSLAA
                     NNLLKAGFYYNTIGLIAMRNNAWDVAAKYFDYAFQSQLSGSLLNKSVALTEDGNLEEA
                     RESWKLLQKINGRETVATAMLQILDTPKDSLMFFDDRIKYQFLRYREDELSSEERRDA
                     LLSFENQAYPERLTLDRAWELFQNMKFMEAQNELANLTVTNDSWKEEYKLLSKAVEVS
                     LRHGSITEVDISLFNSNIKGELPSLKRYLRSVHAEEMNDTVSASAEYELLGFGNPFFV
                     PGIIRAVNYFSKNHPDEFTSYSLLVEAVQYNNRSELLWENYVKECLKRGFDNYADQGM
                     IILKELLNARDYTIMEANYSRWKQEFK"
     gene            complement(8989..9750)
                     /locus_tag="VGA21_00465"
     CDS             complement(8989..9750)
                     /locus_tag="VGA21_00465"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX9649003.1"
                     /translation="MKIVRYIYTGYGLVVFTIGFIVLLPFFLILIQIKSWHKYVFVLN
                     RVWAKWVFGLLGIPMEVEYRERLSRTQRYVFCPNHFSYLDIPTCGLIAHPFIFVGKNS
                     MARIPLFGYMYGKIHITVDRNSLKSKYNTLVKAKAAIDHGKSIVIFPEGGIKTTKMPK
                     LAAFKEGPFRIAIEKQIPVVPVTIPYNWIILPDNENLFNWRKGKLICHEPISTEGMSL
                     ADVDKLKEKVFSVIETELEKYFPSQKTETVEKSEE"
     gene            complement(9768..11117)
                     /locus_tag="VGA21_00470"
     CDS             complement(9768..11117)
                     /locus_tag="VGA21_00470"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015332142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HEX9649004.1"
                     /translation="MRKFLIVLLFICSNISAQEMPVILSMKERAATIDKWLEERIKTV
                     LPQLMRREQIDMWIIAAREYNEDPVIETMLPATWLAARRRTVLIVYDPGPEKQLETLA
                     VARYDVGTQFKRSWEPEKQEDQWQRVVEIITEKNPQKIAINKSAHFALADGLTSSEFD
                     SFMRVLPGESHKKVVSAERLAIGWLETRTQSEMIVYQQICAIAHQVIREGFSSNVIHP
                     GITTTDDLVWWFRERIRELKLVTWFHPSVSIQRADPESFDHLRTFSSRPDNNVIQPGD
                     LLHVDFGITYLRLNTDTQEHAYVLRPGETDAPDYLKKAFEAGNKLQEIFTANFQTGKT
                     GNEILSKSLKEAKDAALKATIYTHPIGFHGHAAGPTIGLWDQQGGVPVTGDYPLYPNT
                     AYSIELNNAYYVEEWKKEIRIMLEEDAFFDGKTVKYINGRQKKLILIPGSAGTILGD"
     gene            complement(11117..11680)
                     /locus_tag="VGA21_00475"
     CDS             complement(11117..11680)
                     /locus_tag="VGA21_00475"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018473012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEX9649005.1"
                     /translation="MENFAKPSRYSETTITELMIPSYANFGGKIHGGILLSLMDKAAY
                     ACASKHAGAYCVTVSVDTVDFLQPVEVGDLVFLKARVNYVGNTSLVVGIKVIAENVKQ
                     GMVKHTNTSYFTMVAKDEDDEPATVPKLILETQDEVRRFMEAIIRKYLKKQQKTEMSS
                     VKNNLKLQDMINKIDEERCVIALKDNL"
     gene            complement(11725..13095)
                     /locus_tag="VGA21_00480"
     CDS             complement(11725..13095)
                     /locus_tag="VGA21_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010854722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEX9649006.1"
                     /translation="MVGEKYIEHFKKNFTLGYPVMLSQLGHVMVGVADSVMVGHLGAE
                     PLAAAALGNVIFYLVMTFGLGISYAITPLVAAADGEGNTTQGGKVLRHGFAINVIASI
                     LIVILVMVGGRILFFLNQPEQVVIQSIPYLYIITFSIIPLMIFQTYRQFAEGLSLTRQ
                     AMFISLAANLLNVGLNYILIFGKFGFDPLGLNGAGIATLISRIVMAGLMFAYVFYRPR
                     FIPFKAEGFHFGSYSKDLFRRMLRIGIPAGFQFLFEVSAFGFAAIMVGWFGANALAAH
                     QIAINLASISYMMATGLSAAATIRVGNQLGRKDLPVMRTAGFTIFFMTILFMSMWAMI
                     FIFGRHFLPTLYIDDTEVIQIATSLLVVAGFFQISDGVQVVGLGALRGMADVKIPTII
                     TFMAYWIFALPMGYILGFYLDLGVVGIWIGLLSGLSVAGILLFLRFNHLTKKMIGSQA
                     ATNRKG"
     gene            complement(13089..13640)
                     /locus_tag="VGA21_00485"
     CDS             complement(13089..13640)
                     /locus_tag="VGA21_00485"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009579300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX9649007.1"
                     /translation="MKKAVNPWKKISGSIVYENNWICVEDHKVINPSGNDGIYGKIHF
                     KNVAVGIIPIDEENYTWLVGQYRYPLNQFSWEIPEGGGIIGGDYLEAAKRELKEETGL
                     VAGNWKEIQRMHLSNSVSDEEAIIYLAENLTQEQAQLDETEYGLEVKRLPFSDVLSMV
                     STGEITDSISVAGILTVARLKRW"
     gene            13699..14298
                     /locus_tag="VGA21_00490"
     CDS             13699..14298
                     /locus_tag="VGA21_00490"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009579302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEX9649008.1"
                     /translation="MLASFHSKNTLEAGCDEAGRGCLAGPVFASAVILTQDYFHEDLN
                     DSKQLSRKKRERLRIEIEANAIAWAIGIADNNEIDEINILNASYLAMHRAIEQLKPQP
                     QLLLVDGNRFNPYPGINHLCIVKGDATFASIAAASILAKEYRDEFMRALAVMHPEYIW
                     DRNVGYPTEEHRNAIRSVGITPFHRKTFQLLPAQFELFE"
     gene            complement(14626..15312)
                     /locus_tag="VGA21_00495"
     CDS             complement(14626..15312)
                     /locus_tag="VGA21_00495"
                     /inference="COORDINATES: protein motif:HMM:NF023521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3575 domain-containing protein"
                     /protein_id="HEX9649009.1"
                     /translation="MRKILQAAFLLTYFTIPALSQEEKEIDRKEAAKITVPNATLKFN
                     IPVLIIDPKHAFFVSTDTRLGNNWSLDMGVGYFFYLNDRAIYKDESFSGVRGRVGIKY
                     YYLFGRRLAPYIGLEGMLNRFNIKEYEQVCRFGCQYWENLIVDKEVKASGISTRTGLQ
                     IFLGEKKRFMFDVYAGLGYKFVEVDENIPADAENFDRLNFFESLYDSGEGDYHVPNII
                     LGIHFGYVFW"
     gene            complement(15316..16077)
                     /locus_tag="VGA21_00500"
     CDS             complement(15316..16077)
                     /locus_tag="VGA21_00500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649010.1"
                     /translation="MKTGKLLIVIIFLIGQACLDGNNFNEAVTGKTGSITRFAVKGDY
                     LYSLDMNQILVFEIFDNGETEQVNAVPADYGLETIIIYDETIYVGSTSALYILDISVP
                     ATPVILSKLDREEILIGGCDPVVVKDNYAYSTVKIIENRCGRLSAVSQLIVYDVSDKK
                     NPFEVNTFPMDQPNGLGYKDNHLFVCDQGVDKIQIFDITVPTNPAYIGFVNLVDPIDL
                     IPNGETMIVSTANDFVIYDITDVNNITRLSSIPKK"
     gene            complement(16084..16656)
                     /locus_tag="VGA21_00505"
     CDS             complement(16084..16656)
                     /locus_tag="VGA21_00505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649011.1"
                     /translation="MGSGVGLTFEHIWSMLKTQGKFLCVLCLAALFGCQLNPDFYNGA
                     GVKPVYISASELSDIRNLEPQPTEKTGPILLLNNLFFMVEQKKGIHVFDVTNLSNPVK
                     ISFIKIPAVNDFSIRNNVLLADNGPHLVSIDITDIYNIVILSIESNVFKQALFPENYS
                     GFFECADPARGIIVDWENAELTNAKCQSFN"
     gene            16701..17216
                     /locus_tag="VGA21_00510"
     CDS             16701..17216
                     /locus_tag="VGA21_00510"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX9649012.1"
                     /translation="MNIFDFIDYQTIESRKLVKSLLNELPAGKWYETPAGINSNIAWQ
                     AGHLVISQYFNAIIVVKGSNKEIQENIPLRQYAIWFAMGSNPTELKEEFTPDVFNKHL
                     DFVAAVAQKVLFSLSEDDLSRPLEPSKMPHPIAKNKLEALSWHFKHEIWHCGQIAMIR
                     RAIGCPVNYFR"
     gene            17260..17661
                     /locus_tag="VGA21_00515"
     CDS             17260..17661
                     /locus_tag="VGA21_00515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649013.1"
                     /translation="MDSHKKILGILYLVWGTLSLLIMFAMSIFFSFVMLNTTHHELGS
                     LEIGLVKTIVTVVCGFIIIIISLPSIIGGLGMIFNQKWAFILILIAGIFSLISFPMGT
                     AIGIYTLWVYFKDQELQRSGGQKEVFEKRNN"
     gene            complement(17658..17906)
                     /locus_tag="VGA21_00520"
     CDS             complement(17658..17906)
                     /locus_tag="VGA21_00520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649014.1"
                     /translation="MKTKKVRTQWDEKTEQWYFSIIDVIEILTDSKLPKRYWSDLKTK
                     LKVEGSKVYEKIVRLKMLAEDGKIRETDVLSSRSRVNF"
     gene            18206..20329
                     /locus_tag="VGA21_00525"
     CDS             18206..20329
                     /locus_tag="VGA21_00525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649015.1"
                     /translation="MAEFRFTGMDHALMIFESSPWLIPLSLLSGAVYAYVLYKPLGPW
                     SLTTRRLLATARFLIVSFLCFLLIGPIIRQIKNNIEDPIVVFTIDNSASVKERIDSAQ
                     LANILDRMEMVRNELESQGFQTQVRTFNESPESNINKNIQFENPFTDLNSLLDNVHRE
                     FEGRNLVASVLMSDGIYNAGVSPTFNSFGFEIFSVGVGDTVVRKDVSVSNILYNQISY
                     QGNIFPMIAEIANRGYDNQLLTVKLSKAGKVIQSRQLNLSGKVSINQVEFQVEATEEG
                     LQHYEISFDALPDELTLKNNRKQAYVDIVAGKEKILILSLAPHPDIKAIRSALDQNRN
                     YETLVFIPGISEEFKDEVETARFDLVVFYEIPDLRSSMLRYYRKFLDSEASILTIVGP
                     TTHLGVFNQLNGLITLQSTRFQPDHVTAFFNTLFTPFQLSDKLQNSLKTYPPVVVPFG
                     KVTNQVEVETFLLQKVGRVETVNPMIVVEPASGKKRAVMLATGFWRWNLNEFLKTEDH
                     EAFNELFIKLVQYLSSREDKRKFKVYPTDTEFTMSEGVRFETEIYNELYERIYNNKVD
                     LTLTNPAGSQTQYSYVPTESQSQYTVRGLDQGIYKYTARTEIGGTQESVSGEFLVREL
                     ELESLNLTADFTLLRNLSKNTGGVFLQEYEIEQLNNILIAKDFPGVIHSAEAYLPLIN
                     MKYLFFIALVLISLEWFTRKYLGSY"
     gene            20418..20852
                     /locus_tag="VGA21_00530"
     CDS             20418..20852
                     /locus_tag="VGA21_00530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649016.1"
                     /translation="MESVVQVVFFKNDEYYVAHCPALGLSVNGSSMHFALHHFEESIN
                     IIHNDLSKKGILVTRLLELGWTIGSAPEHLLLPPYFRAHQKLIEKCPEEKSDFLTCID
                     VKIRIPCSVNSLFRKNLSRSLFDFSSIDGFGPNGNRYPFHLN"
     gene            21328..22617
                     /locus_tag="VGA21_00535"
     CDS             21328..22617
                     /locus_tag="VGA21_00535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649017.1"
                     /translation="MKIHCIFLIIQIAGFSFSCQKSDSDGNQEMKESYLRAYQFMQTN
                     PTEAIKWSDMHIKLALALEDQQQLSKAYFLRAYIYDVILNDPENALKSYLIVLDHSDM
                     GKNNESYFNALANVGELYFRHSYIRTAVEVFGEGLLSAKILNEKSKMNRFVQSLAAAH
                     FKLESWSAAENYIQQGLVLSKEVNDTVSQINYSIMECALLMKNKNFNSVIPKLEFLLK
                     RHGHVSPYQKIRILNNLGINHLSLNQLDKAEFYFNESLLLTEEIRSLSKVTFNNLAKL
                     ALKRKEFGRAEELYHKAELLPISQLGDEDQLNTYDGLVEVYAATDNPRLALQYKQKAV
                     DHIKPLIAQIDKLRSFAGKFEEWQQSREMLETKRNADVSLAFTRIVLIIVIIAIAFGA
                     YLMAQFYRGVRRESELRILREISEYKDAIIRNIRLLF"
BASE COUNT         6825 a         4900 c         4364 g         7023 t
ORIGIN      
        1 gccgtgggaa ccattgattt tccgaaggtc ttttgcctac tttttgacca gaaaaagtag
       61 gtgccgctcc ggcatgagga gaagagaaca aacgagcagc gtataattga gtattgcctt
      121 cgcaaggtct gcacaccccg tcgaaaactc agtaccacgc ggcaggtccc ttacgaagac
      181 cccacggtga cagaagtttt ctaaccctgt ttcacccctt tgtggtttta cggtacgagt
      241 aattctttta ttataataat accatccctt cgggattaaa cattttgata ttttcccctc
      301 cttccgaaac aacctattaa atgaaaacag actgcaaaag gaggggtagg ggtggtttca
      361 gtaacattct aatctctgaa acctctacgc ccaggcgggg tgggcggttt tgcttaacat
      421 tctatctccg ggatttggat ttcgtgttca atattcaaca atctttcacc ccttccgtga
      481 aatcagcgta attcgtttaa tcaatgattc tcatttgtgc gagaaacgtg aggtaatttt
      541 tgatgccgga atcgaatcag aataataaca gcaacgagcg ccagcatagc gcctgaccaa
      601 aatgtttttg caaagtttgc cgggttgttt ccataagccc accccgaata taaagctcca
      661 aggccaattc cgatttccag ggcaatgtag agggtagaca tggcccggcc ccggtgttca
      721 tcgctggcta aatcaatagt ccaggcgaaa atagtggggg atgtcatgcc ggaagcctga
      781 ccaaaaaaaa tcgcggcaat gataaaagta aagtaggtcc tggtgaagcc gagcaaaatc
      841 atggctatca gcaacataat gagcgcaatg agtaaaacta ctctcctccc gtatttatcc
      901 gaggctttac cggcaaagat tctcactgca atggagctta aagtaaaata ggtaaagaag
      961 atgcctttat tttgcaggcc aagatgggtg gaaaagtcag ggattatggt caacaccatt
     1021 ccgaagctaa aggcagaaag aatcattatg agcgcgggaa aatatacgtt tggctccaat
     1081 acttctccgg gagaaatttt caacaggctt aatgaaaatc gttccgtttt tggcagtgac
     1141 tccttcattc caaacaaaac caccacagat aataggccaa gtacagagga aacgtagaac
     1201 atagtattga gcgaaagata aattgcaatc ggactgccga tggccggacc ggcagccatc
     1261 ccgagactga gaaaaactcc cagaataccc aatgcttcac cacgtttttc cctgggaatg
     1321 atatcggcaa tataagcggc tgttccggtt ggtttaaatc ctgtggcaag cccgtgaaca
     1381 agtctcaata taaaaaagcc tgcaaggccg gtgagaaatg gatacatcaa agaagaaacg
     1441 gcggccatca gtgacccgaa caccatgacc ggaattctgc caactttatc ggtgagtttg
     1501 ccgctgaaag gccgggaaag tcctgctgta agggtaaaca gtgaaatgat gagtcctttg
     1561 tattccgctc cacctaattg agtaagatac tccggcagtt ctggaataat catgttaaaa
     1621 ctgcagaaga acaaaaaaga acttaggcat aacagccaga attgcaggtt aaatgttttt
     1681 ggtgttctac tcaattcctg aaagttctac tcaatcctga aattaatttt gcatcagata
     1741 cgcttttata aaatcatcca gatcaccatt caatacattc aatggatcac tgcgttcaat
     1801 tcctgtcctg ttatctttta caagtttata gggatgcaaa acgtaatttc ttatctggga
     1861 tccgaaatca attttcttct tggaactttc gactttatct ctttcggcat tttttttgtc
     1921 aatttcaatc tgatataggc gcgacttcaa aagattcaat gctttttctt tattctgatg
     1981 ttgcgatctc tcctgctggc attctattat caatcctgaa ggcgcatgct ttaacctgac
     2041 tgcagtttct accttgttca cattttgtcc gccggcacca ccggcccgaa atgtatccca
     2101 ggtgatatcc gcaggattta tttcaatatt tatggtttca tcgacaacag gataaacaaa
     2161 aacggatgca aaagacgtgt gcctcctggc gccggaatca aaagggctga ttcttaccag
     2221 cctgtgcacg ccaatttcag actttaaata tccgtaggcg aattcccctg acacttcaat
     2281 ggcagcagat ttgattcctg ctgtttcgcc aggttgataa tagatttgct ttaccttgta
     2341 tccgcttttt tccccccaca taatatacat ccggaggagc atgtctgccc agtcctgact
     2401 ctccgttcca cccgctccgg gattgatttc cactaccgca tctaattgat cttcttcacc
     2461 actaagcatc tttttgaatt ccaaatcttc caatgctttc agcacctttt catattccgc
     2521 atccatttca gaatcctgga tttcaccttc ctggtagaac tcattaaggg tcagcatatc
     2581 ctgaaaatcc ttatcaagtt gttcaaaact gtttgtccag actttctttg attttatttc
     2641 cctcaggatg gcttctcctt ttttaggatc gttccagaaa ccgggttggg tggttagttc
     2701 ttcttcggag gctatttcct ttttcttatt atcgtagtca aaggtacctc ctcaaggagg
     2761 ttacccgttc ctttaaatct tttagttgtt cgtttgtcat gttccggaat tgaagcgcaa
     2821 atgttataaa atgaaggtac tttatcaaat atccacaacc cttaatcgcc agttttttgt
     2881 aagttttttg atgtctgatt tcacaaacat caacagaaac ccggtacctg ccaatgctcc
     2941 cgatgctatt gtaactcctt tattgagact tgcttcattt ccggatacca ctgtttcatt
     3001 aaataaatct cctaaaaaca aaaccactcc cgctactatt aaggtaacag gataacccct
     3061 tgcactgatt cgtcttgtcc gggtgtcatt gatattaata gcattaatgt tttcaatggg
     3121 aaaaaaagcg tagtcaaaaa caattatact atctgctaaa tccgcaatgg ttcccgttcg
     3181 gaagaaatcc tccccattta ggcggtaatt gatttcactg ccaatgtaat actccatccg
     3241 tttcctggtc cccagctttt ctaaaaccag atatttttgg gcaattgctt cttcagtaag
     3301 cagcaaaaag attcccacaa acaatatttt tttcatgagt ataaaaaacc acccgtaaaa
     3361 gcttttagca ggcggtcctt ttcaaaaaaa tcgttgttga attaaacctt gtatacccag
     3421 ttaaatttat cttcaccttt cccgtagcgg atttcattta aagcatcaag caagcggatc
     3481 gagaattctc tttttcccaa gggggggagt ccaaaatctt cttcctcata cccaatcgat
     3541 tcaatatgcg cgatagtagc cgcagtgcct gctccgaatg cttctttcag atcaccattt
     3601 ctggctgcat caataacttc cttaaccgaa acttttcttt cttccacttt taatccccag
     3661 tctcttgcca gtgtaagtac actgtttctg gtgactccgg caagtattga ttcactcagt
     3721 tggggagtga ccaaggtgtc accaataaca aacatcacat tcattgttcc tgcttcttca
     3781 atgtattggt gatcccggcc gtcagtccag atcagctgat gatatccttt ttcctgggcc
     3841 aacttcgcag ggtataaaga cctggcataa tttccggagg ttttggcaaa cccggttccg
     3901 ccctgggccg cacgtgtata tttggtttcg atttttacct taaccggtgt tgcatagtag
     3961 gcacctaccg gacaggtaat gatcatgaat ttgaaggttg cggagggttt cactcctata
     4021 tattcatctg tagcaaaaat aaaaggacga atatacaatg atgttccggg cttttccggt
     4081 acccattgtt tatcaagacg tagtagttct gttaaaccac ccataaaaat ttcttctgaa
     4141 actttgggta tgcacattct ttccgcggaa atatttatgc gcttgaaatt atccaggggt
     4201 ctgaaaatca aaacatcacc acccgggctt ttatacgctt ttaatccttc aaaaattgcc
     4261 tggccatagt ggagcgtagc atttgcagga gttattcgca gatagtcata gggtgagatt
     4321 ctgaaatcct gccatttccc gtctttgtaa tcagccagga acatatgatc agagtagatt
     4381 ttggcaaact gaatgttgtc aaaatcaatt tcactgattc tcgaattttt ggtttccgta
     4441 ataatcaaat ccaatgtttc gatcatgata tttggtttaa cgtgatgaaa ataaattttt
     4501 attaatataa caattttcgt gggattttga agcctatttt ttaggcatcc acggtgtttc
     4561 tgtaataatt aattcatttg catttttacg ggataaaaca aacaggaaat ctgaaagcct
     4621 gtttacgtat ttaatgatta ccggatcaac cttttcttcc gaatttaatt tgattatcca
     4681 cctttctgcg cgccggcaaa ccgacctggc gatgtgtgca attgaaacgg aaacatgtcc
     4741 gccagggaga acaaaatttt tcatttccgg caaagactta tccatttcat ctatcaattg
     4801 ttcaaggtat tcgacatctt gttcgcttat ttcgggaagt tgaatcttgg ttccaggctc
     4861 aacggcgaga tgagacccta tcacaaagag acaattctgg attttcttaa tactttcaat
     4921 ccggcccttg tttacctcct gatcccctac cagccccaga tgcgaattca actcatcaac
     4981 agttccatag gcatgaatac ggatatgaga cttaggtact tttgaaccac caagcaatga
     5041 tgtttcaccg gcatcaccgg ttttggtata aatcttcatt ttaattacac acccattttg
     5101 gcttttggcc ttcgaatgtc cgtgttcctt tcgtctgatt caaccagacc atccctgagt
     5161 ttaacgatcc tgtgtgcata gtgagcaata tcatcttcat gggttaccat aatgatagta
     5221 ttacctgtat catgcaaatg ctgaaacagg tccataatat cataggaggt ttttgaatcc
     5281 aggtttccag taggctcatc agcaaggatt atactggggt cattaacaag agccctggct
     5341 atggcaaccc tttgtcgctg accaccggaa agctcattgg gtttatgttt tgccctgtct
     5401 cccaagccaa catctgttaa tgtctgcatt gctttttcat tacgatctga ttttccatac
     5461 ccggcataaa taagcggaag ggcgacattt tccaacgatg ttgccctcgg taacaaattg
     5521 aaagtctgga aaacgaatcc aatctcttta ttccttattt cggcaagttc attttctgta
     5581 agatcactga catcatgccc atttagaata taggtgccag acgatggagt gtccaggcag
     5641 ccaattatat tcatcagggt tgatttaccg gatccggagg gacccataaa agcaacatac
     5701 tctcctttgt ctattgatat cgtgatggaa ttgagcgctc gaatggtttc acttcccatt
     5761 tgatatatcc ttgaaatttc cttcgtgttt attacctgag gcatctgcaa taatatttgt
     5821 tagtcaaaat taaattaatc tcttaaatct actcaaaaat cgatggttgc cacataaatg
     5881 actatttaaa ctacttgaac ggacgttatc ccctttcagt tgtgcaataa tagggctgaa
     5941 acacgataat tttatttagc ttcaggctac ttaaactctt gtttccacct gctataattt
     6001 gcctccatta tcgtataatc ccgcgcattc aataattctt ttaaaataat catgccctga
     6061 tcagcataat tatcaaatcc tcttttcagg cattccttta cgtagttttc ccaaagtaat
     6121 tcagacctgt tattatactg aacagcttct accagtaaag aataagaagt aaattcatcg
     6181 ggatgatttt tactgaaata atttaccgcc ctgataattc ccgggacaaa aaatggattt
     6241 ccgaatccta acaattcata ttccgcagac gcagacaccg tatcattcat ttcttccgcg
     6301 tgtacactac gcaaatatct ctttaaagac ggcaattcgc ccttaatgtt actgttgaat
     6361 aaagatatat ccacttcagt aatgcttcca tgtctcagag acacttccac agccttcgat
     6421 aacaatttat attcttcttt ccaggagtca tttgtaaccg tcaggtttgc taattcattt
     6481 tgagcttcca taaacttcat gttttggaat aattcccagg cgcgatccag cgtaagtctt
     6541 tccggataag cctgattctc aaaactcaga agagcatctc ttctttcctc tgaggaaagt
     6601 tcatcttctc gatatcttag aaactgatac ttaattcgat cgtcaaaaaa catcaggctg
     6661 tctttgggag tgtcaagaat ctggagcata gctgttgcga cagtttcgcg cccatttatt
     6721 ttttgtagca gtttccagct ttcccttgct tcttcaagat tgccgtcttc ggtgagggcc
     6781 actgacttat ttaagagact tcctgaaagt tgactttgaa aagcataatc gaaatatttg
     6841 gcagctacat cccaggcatt gtttctcatg gcaataagcc caattgtatt ataataaaat
     6901 ccagctttta ataagttatt tgcagccaat gactttagta tggaaatgct tttatcaatc
     6961 cggttatttt tcaacattcc caaagcaagt atatattgaa tttcatccga atatcttgaa
     7021 ttctcaggct tattgctcaa agagtccatc acagcaatga aggcatcatc tccttgaata
     7081 aatgagttca tcccaaagtt tattaattgt gatgaagaca cgagcccaag aatggagtca
     7141 gaaggaaatt gaggtacata atttcccgtt ttttggtttt ggtttagaag tgtggagtaa
     7201 tttatttcag aaatttcatt ccctttgagg taataagatt ctcgtaatga atctggatct
     7261 aattgccaat tgttttcagc caccattgcc agcagattgt tttttccgag tctttcagaa
     7321 attttgaaag cagttgcctg atcgaagaga attgcggccg aatcaagaat ttctgtttgc
     7381 cggaatgcaa gtcctgcatt taccatcagc ggaccacttt ttggaaattg gtcaagacca
     7441 tcccttagag cgaataatcc atttagaaat tgaccgttag ccatcagcgt attgctcaca
     7501 tttatatagg catattcagt aggtcgtttt ttaattgcct gttcgtagaa atatatcgcg
     7561 gttacgtcat tgccttgttt tgaagcgagg gaagcaagag cataattaga ccggtgattt
     7621 tgataaccgt aatcgctccc aagtttgtaa tattgctccg ccaggtagta ttcacgtgtc
     7681 gcggtatata aatcacctat gccattgtaa tatcccgata aggaatgata aatcggtacc
     7741 tcaaagttct cttttagaaa tagagctgct acagcgatca gaccggcaaa tcgaaaggtg
     7801 aaataaggca tgttggttgg cttgtaaatc actttgtata tcccagtctt tttgccaaac
     7861 aaggcgccaa agttggccag tatataaatg aaaaagaaga acccaaaacc caaatgacca
     7921 taaatgatta catcctctat tacttctaaa acaggatcat taccggtaac gaataaatat
     7981 cctgttgtgg ctgtagcaat aatagctact gacaaataaa gcattgggcc ccagggatag
     8041 aaggggacgg cgttcttaag aatttcactt ctggtgatca aaccccaaaa gccgatagtg
     8101 gaagaaagga ttaacagaat ataaggattg atgtagtaga attcccaatc aataacccgg
     8161 gtttcatgaa gatatagcat cataatatta cccaaataga aggcggtaat taccagaaaa
     8221 tgtcgcagtg tatttctgct aactaaagtt gtcgaagatg tgagcagata aatgaatgcg
     8281 ttaataattt catgagaaat catcagaatg aatgccgctg tgataatcaa aactccaagt
     8341 attccataat tggtcaaata caaaaaaggg tgttcgaccc cggcgaaaag ataaaaccca
     8401 aaacaacatg ctgtaactaa aacggcaaac acaagcagtc ttttccaata caatgactct
     8461 ttattcatag catggaaata atagctggtt gggagaagaa tcaggaagac tatgattagg
     8521 ggaattctat caattctccc aaaaatttgc aagtgctcca atttgaggtt gaccataaag
     8581 attatgaaaa ctcccatggc aacgatgtac caaaaacgac ttagttcggt aactgctgat
     8641 aaataaaaaa caattgatat agctatgaac aaaccgaaaa tgtagcttcc cggaagattg
     8701 acctgcagtt cagagccctg gaatttctga aacacaacga aattttcagc tactatgttg
     8761 aaattgaaaa atcccttggt gaaagaatcg aaggttactg ccaaaggttg ttttatgttt
     8821 agaacctgcc aatcaatcac aaaactttca ccccaaagta ttcctgttat caaaaaggaa
     8881 actgataaaa taaataccac agccaataag ttgattgcta tcttataggg tttctcccaa
     8941 tcgttccaaa aaattatatt ctgcatgtca atgattttct ttcaaagatc attcctcact
     9001 tttctcaact gtttccgttt tttgggaagg gaagtatttt tcaagttctg tttcgatgac
     9061 tgaaaatact ttttctttta atttatcaac atctgccagt gacatgccct cggtagaaat
     9121 gggttcatga caaattaatt ttccctttcg ccaattaaag agattttcat tgtcgggtaa
     9181 aattatccaa ttatagggta tggttacggg tacaaccggg atttgttttt caatggcaat
     9241 ccggaatggg ccctctttaa aggcggccaa tttgggcatt tttgtggttt ttattcctcc
     9301 ttccgggaag ataacaatac tctttccatg atctattgcg gccttagcct ttaccaaggt
     9361 attgtattta ctcttcaggc tatttctgtc aactgtaatg tggattttcc catacatata
     9421 cccaaatagt ggaattcttg ccattgaatt tttgcctacg aaaatgaaag gatgagcaat
     9481 aagtccacag gtaggaatat ccagatatga aaaatggttg gggcaaaaga catatcgttg
     9541 ggttcggcta agccgctcac ggtattcaac ttccatagga atccccaata aaccgaaaac
     9601 ccatttagcc catactctat tcaaaacaaa cacatacttg tgccacgatt taatctgtat
     9661 gaggattaga aaaaaaggaa gcagtacaat gaatccgatt gtaaaaacta caagcccata
     9721 tcctgtgtag atataccgta cgattttcat tttattttca gggaatccta atcaccgaga
     9781 atcgtgccgg cgctaccggg gattaaaatc aattttttct gtctgccgtt aatatacttt
     9841 accgtctttc catcaaagaa ggcatcttct tccaacatta tccgaatctc ttttttccac
     9901 tcttcaacat agtaagcatt gttcagttct atggaataag cagtatttgg atacaacgga
     9961 taatcccctg tgacgggcac gcccccttgt tgatcccaga gtccaatggt tgggcccgcg
    10021 gcgtgaccat gaaaaccaat tggatgtgta taaatagtgg ctttcaatgc ggcgtctttt
    10081 gcttccttca gagatttcga caggatctca ttacctgttt tgccggtttg aaaattggca
    10141 gtgaaaattt cctgaagttt attaccggct tcaaacgctt tcttcagata atcgggagca
    10201 tcagtttctc cgggcctcaa cacataagca tgttcctggg tgtcagtatt aagtctcaaa
    10261 taagtaattc caaaatccac atgcagtaaa tcaccaggct gaatcacatt attatccggc
    10321 cggctgctaa atgtcctgag gtgatcaaac gattccggat ctgcccgttg aatggaaacc
    10381 gaaggatgga accaggtaac caatttcaat tcgcgtatac gttccctgaa ccaccaaacc
    10441 aaatcgtctg tagtagttat ccccggatga attacgtttg aagagaagcc ttccctgata
    10501 acctggtgag ctatcgcaca aatttgctga tatactatca tttctgattg agtgcgcgtt
    10561 tccagccatc caatggcgag ccgctctgca cttactacct ttttatgact ttccccggga
    10621 agcactctca tgaatgaatc aaattccgat gaagttaatc cgtctgccaa tgcaaaatgc
    10681 gctgacttat ttattgcaat tttttgagga tttttttctg taattatttc aacgacgcgc
    10741 tgccactgat cttcctgttt ctcaggctcc caggatctct tgaattgagt tccaacatca
    10801 taacgggcaa ctgccagcgt ttcaagttgc ttttcaggtc caggatcata cacgattaag
    10861 acagttctcc tgcgagcagc aagccatgtt gccggcaaca tcgtttcgat taccggatct
    10921 tcattgtatt cacgggcagc aatgatccac atgtctattt gttccctgcg cataagctgt
    10981 ggcaaaacgg ttttgattct ttcctccagc catttgtcaa tagttgcagc tctctccttc
    11041 atggaaagaa taaccggcat ttcctgcgct gaaatatttg agcaaataaa tagcaacaca
    11101 ataagaaatt tcctcattat aaattgtctt taagcgcaat aacacatcgt tcttcatcaa
    11161 ttttattaat catatcctgc agcttcagat tatttttcac cgatgacatt tccgtctttt
    11221 gttgtttttt gagatacttc cggataatgg cttccataaa tcttcttact tcgtcctggg
    11281 tttccaatat caatttggga acagttgccg gttcatcatc ttcatcctta gcgaccatgg
    11341 tgaaatacga agtgttggta tgtttcacca ttccctgttt tacattttcg gcaatcacct
    11401 ttatgcctac aaccagggag gtatttccaa cgtaatttac gcgtgctttc agaaacacca
    11461 ggtcccctac ttcaacaggc tggagaaaat ccactgtatc cacggaaacg gtaacacaat
    11521 aagcgccggc atgtttactt gcacaggcat atgctgcctt atccatcaga gacaaaagta
    11581 tgccaccatg aatcttacct ccaaaattag cataggaagg aatcatgagt tctgtaatcg
    11641 tggtttcaga ataacggctt ggtttggcga aattttccat ggatttaatg tttcaatttc
    11701 aattttgaat atagcaaaaa attattagcc ttttcgattt gtcgccgctt gtgagcctat
    11761 cattttcttt gtaagatgat tgaatcttag aaataacaaa attccggcaa cactgagacc
    11821 tgatagcagg ccaatccaaa ttcctactac ccccaaatca aggtaaaatc ccaatatgta
    11881 tcccatcggc aaggcaaaaa tccaataggc cataaaagta ataatagttg gaatttttac
    11941 atccgccatt ccccggagcg cgcccaaacc cactacctga acgccatcag aaatctggaa
    12001 gaatcctgct accacaagta atgatgttgc aatttgaatt acttccgtat catcaatata
    12061 aagtgttggc aggaagtgcc ttccgaagat aaatatcata gcccacattg acataaataa
    12121 aattgtcata aagaatatgg tgaatccggc agttcgcatt accggaagat cttttctgcc
    12181 aagctggtta ccaacacgga tagttgccgc cgccgaaaga cctgttgcca tcatataact
    12241 tatcgatgcc aggttaattg caatttgatg tgccgccagc gcattagctc caaaccatcc
    12301 caccattatg gcggcgaatc caaaggcgct aacttcaaaa agaaattgaa atcctgcggg
    12361 tatgccgatt ctcaacattc tccggaacaa atctttgctg taactcccaa agtgaaatcc
    12421 ttcagctttg aaaggaatga accgcggtcg gtaaaagacg taagcaaaca tcaacccggc
    12481 cattactatc ctgcttatta aagtagcgat acctgctcca ttcaagccca gaggatcgaa
    12541 tccaaatttg ccaaaaatca ggatgtaatt caatccgaca ttaagcaaat ttgccgcgag
    12601 gctgataaac atcgcctggc gggtaagaga gagtccttcg gcaaactgac ggtaggtttg
    12661 aaatatcatc agcgggatga tcgaaaaggt gattatatac aggtaaggaa tggattgaat
    12721 cactacttgc tccggttgat taagaaaaaa cagaattcta ccaccaacca ttacaagtat
    12781 tacgatcaaa attgacgcaa ttacattaat agcaaatcca tgccgtagta cttttcctcc
    12841 ctgagtagta ttaccctcac cgtccgcggc agcaaccagg ggtgtgatgg cgtaactgat
    12901 tcccaaacca aaggtcatca ccaggtaaaa gattacattg cccagggccg ccgctgcaag
    12961 aggttcagcg ccaaggtgcc ccaccatgac gctgtcagca accccaacca taacatggcc
    13021 gagttggctg agcatgacag gatagccaag agtgaagttc ttcttaaaat gttctatgta
    13081 tttttcccct accatctttt gagtctggca acggtaagga tacctgccac gcttatacta
    13141 tctgtaattt ctccagttga aaccatactc aatacatcgg agaaaggcag cctttttact
    13201 tcaaggccat attctgtctc atcaagctgc gcttgttcct gggttaagtt ctcggccagg
    13261 taaatgatgg cttcttcatc agacacagaa ttacttaaat gcatccgttg gatttctttc
    13321 caattgccgg ctaccaaacc tgtttcttct ttgagttcgc gttttgccgc ctccaggtaa
    13381 tcaccaccaa tgataccccc accttcaggt atttcccatg aaaactgatt caacggatac
    13441 cggtattgac ccaccagcca ggtataattt tcttcatcaa tcgggattat tcccacggcc
    13501 acatttttaa aatggatttt accataaatt ccatcattac cggaaggatt tatcacttta
    13561 tggtcttcta cacaaatcca attgttctca tacactatgc ttccggagat ctttttccag
    13621 ggatttactg cctttttcat agtcgacaaa agtagccaat cttttcagaa gtgattgttg
    13681 caatttatat ttgttgtgat gctggcatct tttcattcta agaatacttt ggaagcaggc
    13741 tgtgatgaag ccggaagagg atgcctggcg ggtccggttt tcgcttccgc agtaattctt
    13801 actcaggatt attttcatga agatttgaac gactccaaac aattatcccg taaaaaaaga
    13861 gagagattaa ggattgaaat tgaagcaaac gcgattgcct gggcaatcgg cattgctgat
    13921 aacaatgaga ttgatgaaat caatatcctt aatgccagtt acctggcaat gcaccgtgcc
    13981 attgaacagc tgaaacccca gccacaatta ttattggtag atggcaatcg cttcaatcca
    14041 tatccgggaa ttaatcattt gtgcattgta aaaggggatg caacatttgc aagtatcgcc
    14101 gccgcttcaa tattagcaaa ggagtacagg gatgaattta tgcgtgctct tgctgtgatg
    14161 catccggaat atatatggga taggaacgta gggtatccta ctgaagaaca tagaaatgca
    14221 attcgttctg ttggaataac gccttttcac aggaaaactt ttcagctgct tcctgctcag
    14281 ttcgaattgt ttgaatagac cgctgcgcca ataagtattt cttccctata tttaaggtga
    14341 attaaatcct aattcaatat cctgtaaaaa attcggcgtt aactcttaat ctcgtggaac
    14401 ctaaatttga ttatttaccg ctttttctgg tacttgccat agcctatctg gttccaattt
    14461 ccctgaaatg gattaggcta acaaaaattc cgtccgtttt gatggcgggg aaaagaaatc
    14521 cacatccctc acggcgacac tcatttaatt gaaggggaca ttattaccgt tattggtgac
    14581 atgcatgcga tggaaaaatt ccgggaaaaa ttcgaatagg taaaattacc agaatacgta
    14641 tccaaaatgt attcccagaa taatattagg cacgtgataa tcgccttcac cgctgtcata
    14701 tagactttca aaaaaattca ggcgatcaaa attttctgcg tccgcaggga tgttttcatc
    14761 tacctccaca aacttatagc cgagccccgc atatacatca aacataaatc ttttcttttc
    14821 ccccaggaat atttgcagac cggttctggt agaaatacca ctggccttaa cctctttgtc
    14881 aactataaga ttttcccaat actggcaccc aaacctgcac acctgttcat attctttaat
    14941 attaaaccgg tttaacatcc cttccaagcc aatataagga gccaatcttc ttccaaatag
    15001 gtagtaatat ttaattccta ccctccctcg aactccggaa aaagattcat ccttataaat
    15061 tgccctgtca tttaaataga aaaagtaacc tactcccatg tccagcgacc aattgttacc
    15121 aagccgcgta tccgtagaaa caaaaaatgc atgctttgga tcgattatca gaaccggaat
    15181 attgaatttt aaggtggcat ttggaacagt aatttttgcc gcttcctttc ggtcaatttc
    15241 tttttcttcc tggcttaacg cggggattgt aaaataagtg aggagaaaag cagcctgtaa
    15301 aatttttcgc aacacttatt ttttagggat tgaacttaac ctggtaatgt tgttcacatc
    15361 cgtaatatca taaattacaa aatcattggc tgtggaaacg atcattgttt caccattagg
    15421 aatcaaatcg attggatcaa ccaggttcac aaatccaata taagcaggat ttgttggaac
    15481 cgttatgtcg aagatctgaa ttttatctac tccctgatcg catacaaaca aatgattgtc
    15541 tttatagcct aagccattgg gttgatccat agggaaggtg ttcacttcaa acggattttt
    15601 cttgtcgcta acgtcataaa caatcaactg acttacggca ctcaatcttc cgcaccggtt
    15661 ttcaattatc ttaacagttg agtacgcgta attatcttta actactacag gatcgcagcc
    15721 accaattaag atctcctccc tgtcaagctt gctaagtata accggagtag caggcactga
    15781 aatatccagg atatacaagg cgcttgtaga gccgacataa atggtttcat cataaataat
    15841 gattgtttcc aaaccataat cagcaggcac agcattaact tgttctgtct caccgttatc
    15901 aaaaatctca aataccagga tttgattcat atccagcgaa tacaaataat cacctttcac
    15961 ggcaaacctg gtaatggaac ctgtttttcc ggttaccgct tcattaaaat tattaccatc
    16021 caggcatgct tgcccgatta gaaaaatgat gacaattaaa agttttcccg ttttcatatc
    16081 ttattaatta aaagattgac attttgcatt tgtcagttcc gcattttccc aatctacaat
    16141 aattcccctt gccggatctg cacattcgaa gaatccggaa taattttctg ggaataatgc
    16201 ttgtttgaat acattggatt caatactcaa aattacaata ttgtaaatat ccgttatatc
    16261 tatacttacc aaatgcggac cattatctgc aagtaacaca ttattgcgga ttgaaaaatc
    16321 gttcacagca ggaattttga tgaatgagat ctttaccgga ttggaaagat tggttacatc
    16381 aaaaacatgg atgcctttct tttgttcaac catgaaaaaa agattattaa gcaaaagtat
    16441 ggggcccgtt ttttctgtag gctgcggttc cagattacgg atatctgata attctgaagc
    16501 agaaatgtat acgggtttta ctccggcgcc attgtaaaaa tccgggttca actgacagcc
    16561 aaaaagcgcg gcaagacata aaacgcaaag gaatttaccc tgggttttaa gcatactcca
    16621 aatatgttca aaagtcaggc caaccccgct gcccaaagga tattaattta acggtatatt
    16681 agaaaagaaa ttgtcagatt atgaacattt ttgattttat tgattatcaa accattgaat
    16741 cgaggaagct tgttaagtca ttgttaaatg agctgccggc cggaaaatgg tacgaaactc
    16801 ccgctggaat taattccaat attgcctggc aggcgggtca cctggtaata agccaatatt
    16861 tcaatgcaat tatcgttgta aagggttcca ataaagaaat tcaggaaaat attcccttgc
    16921 ggcaatatgc gatctggttt gctatgggca gcaatccaac agaactcaaa gaagagttca
    16981 ccccggatgt attcaataaa caccttgatt ttgtggctgc cgtggcacaa aaggttttgt
    17041 tttctttatc agaagacgat ttaagccggc ccctcgaacc ttcaaaaatg ccacacccaa
    17101 ttgcaaaaaa taagttagaa gcattaagtt ggcatttcaa gcatgaaatc tggcattgcg
    17161 gacaaattgc tatgattagg cgcgccattg gctgcccggt aaattatttc agatagttaa
    17221 aatgttgtat ttttgattac acccaaccaa aacaccatca tggattcaca caaaaagatt
    17281 ttaggaattc tctatttagt atggggaacc ctctccttac taatcatgtt tgcaatgagc
    17341 atatttttca gtttcgttat gctcaatacc acccaccatg aattaggttc attagaaata
    17401 ggattagtga aaaccattgt aaccgttgtt tgcggattta tcattatcat catttccctt
    17461 ccttctatta tcggcggcct gggaatgatt tttaaccaaa aatgggcctt tattctgatt
    17521 ttgattgccg gtattttttc tttgatttca tttccgatgg ggacggcaat tggtatttat
    17581 acgctgtggg tttatttcaa agatcaggaa ttgcagagaa gtggtggcca gaaggaagtt
    17641 tttgaaaagc ggaataacta aaagttcact ctgcttcttg aagaaagtac atcagtttcc
    17701 cgtattttgc cgtcttcggc cagcattttc aaccgtacga ttttctcgta cactttactt
    17761 ccctcgactt tcagcttggt cttcaggtcg ctccagtaac gtttcgggag tttactatca
    17821 gttaaaattt caattacatc aataatagag aaataccatt gttcagtttt ctcatcccat
    17881 tgagttctaa cttttttagt tttcaaataa ttttatgttc gacataggtt gtactcttcc
    17941 tagatttaaa atatttccct gattttagtt tctatttttc gttcaaagat ttcaatgatg
    18001 cgtttgttcg tagataccaa ttgcatttct tcttcaatat ctgacctacc attcttgtta
    18061 tcaaagaatc atatttgaga agagtttatt actttgtccc aactcacata aaagttaccc
    18121 tatttaatat tctttttgag gttggcaaac ttattgccgc aatttatctg agtagttggc
    18181 cggaaaatcc ctggcaaacc ttattttggc agaattccgt tttactggta tggaccatgc
    18241 attaatgatt tttgaaagca gtccctggct gattcccctg agcttgttgt cgggagcagt
    18301 ttatgcctat gtcctctata agccgcttgg accctggtcc ctcactaccc ggagactact
    18361 ggccacagcc aggtttctga tagtgagttt tctatgtttc ttgttgattg gtccaattat
    18421 aagacagata aaaaataata ttgaagatcc catagttgtc tttaccatcg ataactccgc
    18481 ttcagtaaaa gaaagaattg actctgccca actggccaac atactcgata ggatggaaat
    18541 ggttcgaaac gaactggaat ctcagggctt ccaaacccag gtaagaactt ttaacgaaag
    18601 tcccgaatca aacataaaca agaacattca gtttgaaaat ccttttactg atctgaattc
    18661 gctgctggat aacgttcacc gggaatttga aggtcgtaat ctggtggcat ccgtattgat
    18721 gtctgatgga atttacaatg ccggagtttc gccaactttt aattcttttg ggtttgaaat
    18781 attttcggta ggcgttggcg acacggtggt tcgcaaagat gtttccgtaa gtaatattct
    18841 ctacaatcaa atctcttacc agggaaatat atttcccatg attgctgaaa ttgccaatag
    18901 aggttacgac aatcagttgc ttacagttaa actctctaaa gcgggaaagg taattcaatc
    18961 aagacaattg aatttgtcag gcaaagtaag tatcaaccag gtagagtttc aggttgaagc
    19021 aactgaagag ggccttcagc actacgaaat ttcctttgat gcccttccgg atgaattaac
    19081 cttgaaaaat aaccggaagc aggcctatgt agacattgtt gcaggtaaag agaaaattct
    19141 gattctttcc ttagctcctc atccggatat aaaagctatt cgtagtgctc tggaccaaaa
    19201 tcggaattat gaaaccctgg tttttattcc gggaatctct gaagaattta aagatgaagt
    19261 agaaaccgcc aggttcgacc tggtagtatt ttatgaaatt ccggatttac gaagttcgat
    19321 gctcagatat taccggaagt tccttgattc agaagcttcc atactgacta ttgtgggtcc
    19381 aactactcat cttggagttt ttaaccagct aaacggttta atcaccctgc aaagcacacg
    19441 gttccagcct gaccatgtca ctgcattttt taacacgctg tttacgccct ttcagctttc
    19501 ggataaactt cagaacagcc tgaaaacgta tccacctgtg gttgttccct tcggaaaagt
    19561 tacaaatcag gttgaagtgg aaactttctt gttgcaaaaa gtcggccggg tagaaactgt
    19621 taatcccatg attgtggttg agcccgcatc aggaaagaaa cgggccgtaa tgttagcgac
    19681 gggtttttgg agatggaacc ttaatgaatt tctaaaaaca gaggaccacg aagcttttaa
    19741 tgaattgttt attaagctgg tgcagtacct gtcatcgcgg gaagacaaaa gaaagttcaa
    19801 ggtataccca accgatacgg aatttacgat gtcggaaggt gttcgatttg aaaccgaaat
    19861 ttacaatgaa ctctatgaaa gaatttataa caataaggtt gatttaactc tgacaaaccc
    19921 tgcaggatct caaactcaat attcttatgt acctacggaa agccaaagtc aatatacagt
    19981 tcgcggactt gatcaaggca tttacaaata tactgcccgg acagaaatag gcggaacaca
    20041 agaatccgta tccggtgaat ttctggtcag ggaacttgaa ttggaaagtc ttaatttgac
    20101 cgccgatttc accctgcttc gaaacctttc aaaaaatacc ggcggcgtat ttttgcaaga
    20161 atatgaaatc gaacaactga acaacatact catcgctaag gattttcccg gtgtaattca
    20221 ttcagcggaa gcttatctgc cattgattaa catgaagtat cttttcttta ttgccttggt
    20281 tttgatttcc ctggaatggt tcacaagaaa atatctgggg tcgtattaat atacaataaa
    20341 acttacatct gtctgattgg tgtagcagaa tctcaattat tttacaatct tgcagctcat
    20401 ttatgataca ctcaaatatg gaatcagttg tacaagtagt gttctttaaa aatgatgaat
    20461 attatgtagc ccattgtcct gccttaggtt tatctgttaa tggaagttcg atgcatttcg
    20521 ctttacacca tttcgaggaa tccataaata tcattcacaa tgacctgtct aaaaaaggaa
    20581 tcctggtcac aagactgctt gaattggggt ggacaatcgg ttccgcaccg gaacatttac
    20641 ttctacctcc ttatttccgc gctcatcaaa agctgattga aaaatgcccg gaggagaaat
    20701 ctgattttct aacctgcata gacgtgaaaa tcagaatccc gtgctctgta aattcccttt
    20761 ttcggaagaa tctgtccaga tccttatttg atttttcttc aatcgatggc tttggaccta
    20821 atggtaatag atatcctttt cacctgaatt gagggaattt aaaccttagg atttggcacg
    20881 ttcatgaatg ttgaatcctg aatccacaaa aaatcaggga gcttgaaatc ggtaataccg
    20941 attcgatgat actctttgag ggctcgttat acaattataa tgtcaatata atattagtat
    21001 atttatatta attgtttgat ttattatatt attctcttac tattacatca ccaactaaaa
    21061 aaattacaat tatgagaaag ctaaaaatta ccatggtggc actgggggtg accttctgcg
    21121 tatcctcatg ttccttaacc gaggatggca ttgtccccca gaaagaagta ttgagaagca
    21181 aggatggaat tatgatccct gagccccaac caataccgcc tcctcctcct gatattttat
    21241 attagtgggg aaccgataga taaaatataa ttttaactat cttaatttat actatttatt
    21301 atatttgtat aaattattcg acctaatatg aaaatacatt gtatttttct aataattcag
    21361 atagcaggtt tttctttttc atgtcagaaa tcagattctg atggtaacca ggaaatgaag
    21421 gaaagttact tacgtgcata tcaatttatg caaactaacc ccactgaggc tattaagtgg
    21481 tctgatatgc atataaaatt ggctttggca ttggaggatc agcaacaatt gtcaaaggct
    21541 tattttcttc gtgcatatat atatgatgtc attttgaacg atcctgaaaa tgctttgaaa
    21601 tcttacctaa ttgtattgga ccattctgac atgggaaaga ataacgaaag ttatttcaac
    21661 gcattggcca atgttggtga gttgtacttc cgccattcat acataagaac tgcggtggaa
    21721 gtgtttggag aaggattatt gagcgctaag atattaaatg agaagtcaaa gatgaacagg
    21781 tttgttcaga gtttggcggc tgcgcatttt aagctggagt cctggtccgc tgcggaaaac
    21841 tatattcagc agggtcttgt tttaagcaag gaagttaatg acaccgtgtc gcagattaat
    21901 tattccataa tggagtgtgc gttactcatg aaaaataaga atttcaattc cgttatacca
    21961 aaacttgaat tcctgttgaa gcgtcatggg catgtttctc catatcaaaa aatcaggatc
    22021 ttgaacaacc tgggcatcaa tcacctttca cttaaccaac tggataaagc tgaattttac
    22081 ttcaatgaat ctttattgtt aacagaagaa atcagaagtc tctcaaaagt aacttttaac
    22141 aacctggcaa agctggcatt aaaaagaaaa gaattcggaa gagccgagga gctttaccac
    22201 aaagctgagt tgttaccaat atctcaattg ggtgacgaag atcaattgaa tacctatgat
    22261 ggtctggttg aagtctacgc agccacagat aatcccaggc tggccctgca gtataaacaa
    22321 aaagcagtag atcatattaa gccgttaatc gctcaaattg ataaactccg ttcttttgca
    22381 ggtaaattcg aggagtggca gcaaagccgg gaaatgcttg aaactaagag gaacgccgat
    22441 gtcagccttg catttacccg gatagtttta attattgtca tcattgctat tgcgttcggc
    22501 gcctacctca tggctcaatt ttatagaggg gtgaggcgtg aaagcgagtt aagaatatta
    22561 agggaaatca gtgagtataa ggatgcaata atcagaaata tcagactttt gttctagaaa
    22621 ttaaatcaca attctgtttc gtacaattga agccgttccc acaccggggg cggctttttg
    22681 tgctttatga atagttacgg atttgcgcgc aaaacttctt ttctccacag atttcgcgga
    22741 tttgcgcaga agactccctg aacaattttt tccctgcgat gatctgcgat atcagcgggg
    22801 actccataaa atttctctca caaagatcaa ctaacggaat tttcacagaa aacttctttt
    22861 ctcccgcaga tcgacgcgga tttgcgcaga aatctctgaa taattttttc cctgcggtga
    22921 tctgcgatat cagcggggac tcaataaaaa tttttctctc aaaaatcaac taactgattt
    22981 tcatagaaaa cttcttttct cccgcagatc gacgcggatt tgcgcagaag actccccgaa
    23041 caattttttc tgcggtgatc tgcgatatca gcggggactc aataaaaatt tttctctcaa
    23101 aaatcaacta ac
//
LOCUS       DASWHX010000011         1517 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_1856234, whole genome shotgun sequence.
ACCESSION   DASWHX010000011 DASWHX010000000
VERSION     DASWHX010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1856234"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..416
                     /locus_tag="VGA21_00540"
     CDS             <1..416
                     /locus_tag="VGA21_00540"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEX9649018.1"
                     /translation="NMKRDISKKRLLDYPIEQVWQCLTDPKLLSWLGEAEIELKEGGK
                     VTFRENGNEYHGEILTVQKPINLAYSWNDPGHSFTSYVWWKLMDKKGQTLLELEHSGF
                     KGIMGILASYSYKSCWSRKFRNLEKTLKEIVIETT"
     gene            453..788
                     /locus_tag="VGA21_00545"
     CDS             453..788
                     /locus_tag="VGA21_00545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649019.1"
                     /translation="MRIFQGQKWATFHYLGDICNENYGGIIIGFPSTMKKSRTKIRDL
                     ILLIGVLVAIFAGIYSAYNSLEKEPVTEPAQQESKISNETTGSLVTGILALVFKNGLE
                     LVQVMRESN"
     gene            820..>1517
                     /locus_tag="VGA21_00550"
     CDS             820..>1517
                     /locus_tag="VGA21_00550"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HEX9649020.1"
                     /translation="MKGVGKIISVLCFLYLPAIAMAQGNEYKLDEVYKIKDNGKIELY
                     TDDADIQITGTDRSDVRVKVYYRLDTRGFAFGNKEFELDISESGGDLIIRENSYGSTG
                     VFFGTVTEQYEITIYAPKGISLVLKGDDDDYRIRGIDGSIAINSDDADIVITECNGNH
                     FEFDIDDGDLEMDGGSGFLYVRSDDGDLIFRNSKFSEVSAILDDADMLLETSLTDGGD
                     YTFKVDDGDLDMRIL"
BASE COUNT          480 a          240 c          362 g          435 t
ORIGIN      
        1 taaatatgaa aagggacatc agcaagaaac ggcttttgga ctatcctatt gagcaggtat
       61 ggcaatgcct tacagatccg aaattacttt cctggttagg tgaagcagag attgaactca
      121 aagaaggcgg caaagtaaca ttccgtgaaa acgggaatga atatcatgga gagatcttaa
      181 ctgtacagaa acccattaat ctggcttata gctggaatga tccgggacat tcatttacca
      241 gctatgtttg gtggaagctt atggataaga aaggtcaaac cctgctagaa cttgagcatt
      301 ccggatttaa gggtataatg ggcattttag ctagttattc ctacaagagt tgctggagca
      361 ggaaatttag aaatctggag aagacactaa aagaaatagt gattgaaaca acctgaggaa
      421 atgtccgttt ttgagcgcaa aagtgtacga aaatgcgcat ttttcaaggc cagaaatggg
      481 ccactttcca ctatttaggt gatatttgta atgaaaatta tggtggcata ataattggct
      541 tcccttcaac aatgaagaaa agcaggacta agattagaga cttaatactt ttgattggtg
      601 tattggtagc catttttgcc ggtatatact ctgcatacaa ctctcttgaa aaggaacctg
      661 taaccgagcc cgcccagcaa gagtccaaga tttcaaatga gaccaccgga tccctggtta
      721 ccggaattct tgctctggtt tttaagaatg gtttggagtt ggtccaggtg atgcgcgaat
      781 caaattaatt ataacctaaa acttaattta acatttgata tgaaaggtgt cggaaaaatt
      841 atttctgtat tatgtttttt atacttgcca gccattgcaa tggcccaggg aaacgaatat
      901 aaacttgatg aagtttataa gataaaagat aatggaaaga ttgagttgta cacagatgat
      961 gctgacattc agatcaccgg cacagaccgg tcagatgtca gggttaaagt ttattaccga
     1021 ttagatacac ggggatttgc ttttggaaat aaagaatttg agctggatat ttctgaaagt
     1081 ggcggagatc tgattatccg tgagaattct tacggcagca ccggagtttt cttcggcact
     1141 gttacagagc aatacgaaat aaccatttac gcgccgaaag gtatcagcct cgttttgaag
     1201 ggagatgacg atgattaccg gatcagggga attgatggtt caattgcaat caacagtgat
     1261 gatgcagata ttgttatcac tgagtgcaac ggaaatcatt ttgaatttga tattgatgat
     1321 ggggatttgg agatggatgg aggaagtggg tttttatacg tgaggtctga tgatggagac
     1381 ttgattttta gaaatagcaa attcagtgaa gtaagcgcca ttcttgatga cgccgatatg
     1441 ctattggaaa cttcattgac cgatggaggt gattatacct ttaaagttga tgacggcgac
     1501 cttgatatgc gaatact
//
LOCUS       DASWHX010000012         9678 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_2964505, whole genome shotgun sequence.
ACCESSION   DASWHX010000012 DASWHX010000000
VERSION     DASWHX010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 9678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9678
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2964505"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1613
                     /locus_tag="VGA21_00555"
     CDS             <1..1613
                     /locus_tag="VGA21_00555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HEX9649021.1"
                     /translation="HALKEKYLLEMRELTLEGRTNKSLFNELNAIKKTLTRTAIWAVM
                     NTAKKIVYVNKLFSEISGYKESELIGKHHTISNDGLYEEEFWEGILEKVRKGNAWRGE
                     VKCKRKDGSIYWTDTLIGPIMNNEGEFENYLTLKFDITEKKNMEEQLSQKNEQLNLLN
                     SKMEEVNANLATQQNAMESSMLVLKGDKNCNITFANKHVLNLFGFSEAELLSNCVIID
                     PAFKENPAQEQIKKLLEKGEIWKGELRCSNKTGEPVWLHLAIAPIFIQDEITSLLMIG
                     FDATARKKMEDRIIRQKQELIKVNQSKDKLFGLVAHDLKSPLASLEGVLELMAAGELT
                     KSETQLLTGKIGEHLGKVNELLTNLLNWSRSQLNGIKVRPSKLNARNIAKDTIDLLSD
                     VAAKKAIIIENKISPNLNILADQTMITTVFRNLLVNAIKFSKQGGLIQVSAQEKKETN
                     TIFISDNGLGIDYKHQKKLFTDFTVSREGTTGEIGSGLGLQICKDFLEKNNGTLDFES
                     TPGKGSTFYFELPKFKAGRFQKLVPVAG"
     gene            2287..4356
                     /locus_tag="VGA21_00560"
     CDS             2287..4356
                     /locus_tag="VGA21_00560"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9649022.1"
                     /translation="MTASGEIHNNREFAQVKAILIGISIVIALSGLLNLLGWKYDIEI
                     LKRPIQDFDATNPLTAILFLMFGISMVLVHLNKISKKIGLIIGALIIIISVFHAMDII
                     LQTGVNIDKIIFSDNLESDLSDYNNSNRMAFNIALSFVLLGTIFLLSAGNTRKKKIAN
                     ELSLVIILIGAISLLLSAFRFEKVLELQAIIPMGAYSACLLILCALAIIVSNYNVGFM
                     KIILAKNSGGRFARIYIPISFMGPLLMGYAGIILTRETFLNIESSFAIIIVLATIIYF
                     LVTLQTASLLSSKESLQKDAKKYISEYELGMRQVIEQSPSPLIAVDKRGAIQIVNAEA
                     RDLFQYQEKELLGKKVEVLIPESMNQKHESYRDKYMKNPVSRPMGKLLELKGKKKNGE
                     EFPLEIALKPIQFKSESDVLIAASILDITMRKEQEILIRDLHANLEDKIKERTAQLED
                     ANRELESFAYSVSHDLRAPLRALEGFSKLLFSKYGESFDTESARWLKYINENAIKMDI
                     LINDILHFSRVNRKELIKGEFDIRNLIEEVMEECLQPYAGKPMDVDLNIEHPIINGDR
                     QALRMVWQNLIGNALKFSSKEDVIRIKIKVKKAGKIIQCSIEDNGAGFDMKYSNKLFG
                     VFERLHKESEYEGTGIGLAIVNKIVKKHNGRIWAKSKVGKGAKFTFECENVSEIHKPT
                     DSNPIKN"
     gene            4399..4842
                     /locus_tag="VGA21_00565"
     CDS             4399..4842
                     /locus_tag="VGA21_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002693983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9649023.1"
                     /translation="MDTKPVEILLVEDRPEDAEMTIMAIKDAKLVNNIHWVEDGEEAL
                     DFIFGRGEYSSRKASPLPSLILLDLKLPKVNGLEVLAKIKQDPKTKKIPVVILTTSKT
                     SREVEEAYTLGANSYIIKPVDFEKFVTTVREIGLYWILVNYFPEK"
     gene            5146..6594
                     /locus_tag="VGA21_00570"
     CDS             5146..6594
                     /locus_tag="VGA21_00570"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9649024.1"
                     /translation="MEIKKSDKRIKEILAALLMFAKGDNSEQLKISDNHDEIDSIMMA
                     VNSLRESVREKDLEMEERKNKTKVLIELLHKYSALDFSERAVISKNGDEIDSIAAGLN
                     AVIDEWQSKLEKTEESVEKIRTIFKHAPDAIIVINSRGVVEQWNPAAEEIFGWSEDEV
                     MSKPIHTIIIPENYREKHLNGMNRFLKTGKSSVTKLQLKSLRKNNEMIDVELTISSAM
                     VKGEYLFIGFLRDISKKIKAEQEIGKRTIQLETANKELEEFAYTVSHDLRGPLRTLEG
                     FSKLLFDKYNERLDSDATRWLNFINKNALKMDLIINDLLEFSIISRQEIILDRFDTKS
                     LIDGVMKECLSSYKKIKVEPKVDIEFPTTYGDRSSLRKVWQHLINNALKFSSKKDVVK
                     LEIQVKRAGNAIIYSIEDNGTGFNMEHSDKIFGVFERLHKESEFEGNGIGLAIVSKII
                     EKHNGKIWIESKVGGGTKFYFELEFQDVPIKS"
     gene            complement(6665..7561)
                     /locus_tag="VGA21_00575"
     CDS             complement(6665..7561)
                     /locus_tag="VGA21_00575"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HEX9649025.1"
                     /translation="MRFTAFIILLALSTIQSFGQTEPLISTYTMTRGWINPAQITSNP
                     ADLQVKLLHRSQWTAMDNSPSTQLFSVENKFRGNKGIGFYLSNDQLGFEKITSVSLHY
                     SQSINFGGFMISLGLGAGVANRKLDGSKLRYQNPDPNAIESDQNESTPDFNFGFAIHL
                     GKLTVSTSLKHLLYSRNNDDFFKISRQFFAEGYYQLALSQHIITEPSISYKETGFTRK
                     VGFGSTTTFYDKISAGINYHSGKEANFIFGMNISQTIQINYSFQHIFSDDVYVRPDSH
                     EVSLVLSFNKHHTKLFQTPRYL"
     gene            complement(7567..>9678)
                     /locus_tag="VGA21_00580"
     CDS             complement(7567..>9678)
                     /locus_tag="VGA21_00580"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX9649026.1"
                     /translation="LAIQQFKELVPKGSNYTQKEQEIIYYLAESYRAVSDYETSLKWY
                     TKLIRVGYKYVDLAVYAKLLISNSKYGEAREYLLRSNVKFNPEFQHLLITADNGLAIT
                     SEDTLYAVSNVKKLNSRYSDYGVSYLDGKVFISSSRINSNSTAIYDFNGQGYSNIYAA
                     NGGSPELVQGELNSEFNDGTLVYSTVLGYGFYTQCNGYEGNGKACNIYYSKYNVNSDT
                     WSKGILLEAINSKYNSGHPAISTDGTTLIFSSDLPGGLGGNDLWKIARTGSVWGNPEN
                     LGPEVNTPGNELFPSLYNDSLLYFATDGRPGYGGLDIYEFNLAAQNSEPALLKLPVNS
                     SADDFGLIMIKPDSGFFVSNRKGGLGDDDIYQFKTITPPQLLQTEEFVVKGEVTEAGT
                     GVLLPGALVILEARGYVDSVLTDSNGKFEFENRQDIIASGDGFSLKVSKGQYLNDFME
                     LSSEKDLFTSELKFELIQFQKDEISIENIYYDYGKYSLRKESYASLNKLSGVLKANPM
                     LAVQINAHTDSRGSNHYNLNLSQKRAESVVTFLLNQETPAERLQARGWGEELPLVSKA
                     MDEYEHEINRRTTFKITNYEEVKDSYSDTKKPGLIVGNSIKDELNAAVYKIKIEAADK
                     KINPLALTKLQGNFPEYEINYKKEINGTYSFTIGEFIDINEAESFLEKVRNFGHEAFV
                     IALINGKEVPFGKRKLSTAKL"
BASE COUNT         3273 a         1700 c         1913 g         2792 t
ORIGIN      
        1 ttcatgcttt gaaagagaag tatttactgg aaatgaggga actaacacta gagggccgaa
       61 cgaataaatc cttgtttaat gaacttaatg ctattaagaa aacccttacc aggacagcta
      121 tatgggcggt tatgaatacg gccaagaaaa ttgtgtatgt gaacaagtta tttagtgaaa
      181 tctccggata taaagaatct gaactgattg gaaaacatca tacgataagc aatgacggat
      241 tatatgaaga ggaattttgg gaaggaatat tggaaaaggt gaggaaggga aatgcgtggc
      301 gaggtgaagt aaaatgcaag agaaaagatg gctctattta ttggactgat accttaattg
      361 gacccataat gaataacgaa ggtgaatttg aaaattatct gacgctgaag tttgatatta
      421 cggaaaagaa aaatatggag gagcaactct cacaaaagaa cgagcaatta aacttattaa
      481 actcaaagat ggaagaggtg aatgcaaacc tggctactca gcaaaatgcc atggaatctt
      541 ctatgttagt tctaaaaggt gataagaatt gtaacattac ctttgccaat aaacatgttt
      601 tgaatctttt tggtttttca gaagcggaat tactaagcaa ttgtgtaatt attgatcctg
      661 cttttaaaga aaatcctgct caggagcaaa ttaaaaaact attggagaaa ggagaaatct
      721 ggaaaggtga gttgagatgc agtaataaaa ccggggaacc cgtctggctt catttagcca
      781 ttgctcccat cttcattcaa gatgaaatta catcattgct tatgatcgga tttgacgcga
      841 ccgcgaggaa gaaaatggaa gatagaatta tccggcaaaa gcaggaactg attaaagtga
      901 atcaatccaa ggataaacta ttcgggctcg tggcccatga cctgaaaagt ccgctggcct
      961 cccttgaagg agtcctggaa ttaatggcag caggagaatt aacaaaaagc gaaacccaac
     1021 tcttaacagg caagattggg gagcatttgg gtaaagtaaa tgaactgcta accaatcttt
     1081 taaattggtc cagaagtcag ttaaatggaa tcaaggtgcg gccatcaaaa ttaaatgcgc
     1141 gcaatatagc aaaagacacc attgatctgt taagtgacgt ggcagccaaa aaggccatca
     1201 tcattgagaa taaaatttcc ccaaacctga acatcctggc cgaccaaact atgataacaa
     1261 ctgtattcag aaatctgctg gttaatgcta taaaattcag taaacagggt ggccttattc
     1321 aggtaagcgc tcaggaaaaa aaggaaacca ataccatatt tatttcagat aatggcctgg
     1381 gaattgatta caaacatcaa aagaaattat ttacagattt cacggtaagc agggaaggaa
     1441 ctaccggtga aatagggtcc gggctgggtc tgcaaatttg caaagatttt ttggagaaaa
     1501 ataatggaac attggacttt gaaagtaccc ccggtaaggg aagtacattc tattttgaac
     1561 tgccaaaatt caaagcaggc cgttttcaaa agttggttcc tgtggccggt taagtgtatc
     1621 gtggcatact ctcaggtaaa cccttagcga tacaaatttg aataagtatc cccacacctt
     1681 catttattcc cgtcaaaaat tccattgcca ataattattg aaggtgagga gtggcatact
     1741 ctcaggtaaa cccttagcga tacaaatttg aataagtatc cgcacacctt catttattcc
     1801 tgtcaaaaat tctattgcca ttgatattga aggtgaggtg tggcacactt tcaggtaaac
     1861 cctcagcgga tatacttgta cttaatgagc gcagcagcgt aaaattaacc atccaataat
     1921 gagtcttaag cggacttatt agattggctc gcccttaaat tgtatggtta aattacatgg
     1981 aactacttgc ccaaaaataa tgcctgaagg ggctggaaac aggaaattca attttacaat
     2041 gatggttttg aacaaaaaag atatgcattt taaaacacat tagtataaaa ccttacctga
     2101 atgatttttg tttgaaaatt caacttagaa aataattttt tatgctataa tattaatgta
     2161 ttatgatatc attattttcc ttaaggaaga ctttttcagt cccgctacca ccaggttcat
     2221 tataataact taataaaaat atttattacc accaatttgg gtgaaataaa tagaattatc
     2281 tgaacaatga ctgcatcggg agaaatacat aacaacaggg agtttgccca ggtaaaagca
     2341 attctcattg gtatttcaat agtaatcgct ttatcggggt tactgaattt attaggatgg
     2401 aaatacgata tagaaattct aaagaggccc atccaggatt ttgatgcaac aaatccattg
     2461 accgcgattc tttttctaat gttcggaata agcatggtgc ttgttcactt aaataagatc
     2521 tcaaaaaaaa ttgggctaat aatcggagcc ctgataatca taatttcagt tttccacgca
     2581 atggatatca ttctccaaac tggtgtgaat attgataaaa ttatattctc cgacaatttg
     2641 gaatcagacc ttagcgatta caacaattca aaccgaatgg cgtttaatat cgctttgagc
     2701 tttgttttac tcgggaccat ttttttattg tccgccggta atacgagaaa gaagaaaatc
     2761 gcaaatgaac tttcacttgt cattattctt ataggcgcaa tttccctgtt gctaagcgct
     2821 ttcaggtttg aaaaagtgtt ggaattacag gcaataattc ctatgggcgc atactcggcc
     2881 tgcctgctta ttttatgcgc attggctatt atagtcagta actataatgt gggatttatg
     2941 aaaataatcc tggcgaaaaa ttcaggagga agattcgcac ggatctatat tcctatttca
     3001 tttatgggtc ccttactgat gggttatgca ggaattatac taaccagaga aactttttta
     3061 aacattgaat cgtcatttgc catcattata gttctggcaa caattattta ctttttggtt
     3121 accctgcaga ccgcatcttt gttgtcgagt aaagaatctc tgcaaaaaga cgccaaaaaa
     3181 tacataagcg agtatgaact tggaatgagg caggttattg agcaatctcc aagtccttta
     3241 atagcggtag ataagagggg agcaatccaa atcgttaatg cggaagccag agatctcttt
     3301 cagtaccagg aaaaagaact tctgggaaaa aaagttgaag ttctcatccc tgaaagtatg
     3361 aatcagaaac atgaatcata tagagataaa tatatgaaga atcctgtttc caggcccatg
     3421 ggaaaattac ttgagttgaa aggcaagaaa aaaaatggcg aggaatttcc actcgagatt
     3481 gccctcaaac caatacaatt caagtcagaa agtgatgtgt tgattgctgc aagcattctg
     3541 gatattacaa tgcggaaaga acaagaaatc ctgataagag atttacacgc caacctggaa
     3601 gataaaataa aagaaagaac agcacagttg gaggatgcaa acagggaatt ggaaagtttt
     3661 gcctactccg tatctcacga cttaagagct ccacttaggg cattggaagg cttttcaaaa
     3721 ttactattta gcaaatacgg ggaaagtttt gataccgaat cagcaagatg gttaaaatac
     3781 atcaatgaga atgcaattaa aatggatatc ctcataaatg acattcttca tttttcaagg
     3841 gtaaatcgaa aagagttaat caagggagag tttgatatta gaaacctgat tgaagaagta
     3901 atggaagaat gcttgcaacc atatgccgga aaaccgatgg acgttgatct aaatattgaa
     3961 cacccaatca tcaatggtga ccgccaggct ttaagaatgg tttggcaaaa tctcatcggc
     4021 aatgcgctca aattttcatc aaaggaagat gtaataagaa taaaaatcaa ggttaaaaaa
     4081 gcaggcaaaa tcatccaatg tagtattgaa gataatggag cgggattcga catgaaatac
     4141 agtaataaac tttttggagt tttcgaaaga ttgcataagg aatccgaata tgaaggcact
     4201 ggaattggtt tggcgattgt aaataaaatt gtgaagaagc ataacggaag gatatgggcc
     4261 aagagtaaag ttgggaaggg cgccaaattt acttttgagt gcgaaaatgt gagcgaaata
     4321 cacaaaccaa ctgacagtaa cccaataaag aattaagacc gaaaattaat aacaaataaa
     4381 cctctaatct caaaccccat ggataccaaa ccagttgaaa ttttgctagt ggaagacaga
     4441 cccgaagatg ctgaaatgac tataatggcc attaaagatg ccaagcttgt taataacatt
     4501 cattgggtgg aagacggaga agaagctctg gattttattt ttggccgcgg cgaatatagt
     4561 tcccggaaag ccagtccttt gccgagccta atcctattag atcttaaatt gccaaaggta
     4621 aacggattgg aagtgcttgc taaaatcaaa caagatccaa agacaaaaaa gatccccgtg
     4681 gttatactta caacctcaaa gacgtcacga gaggttgaag aagcctatac tttgggagcc
     4741 aacagctaca taataaagcc tgttgatttt gagaagtttg ttaccacagt ccgggaaata
     4801 ggattgtatt ggatcctggt aaattatttt cctgaaaaat aataaaattt cgaaagccgg
     4861 cacagcatat tataatttcg ttagcgaaga caaaaagtgt aaggggtcat ttctcaaaag
     4921 aatttcaaac ctcaaatcaa actaaaacgg tcccaagact cacttcaaaa gacttttttg
     4981 aagaaagtct attgcttacc attaattatt gataaatttt ggaatactac ccaaatgctc
     5041 aatttccggt tcaattcctt cattaattta aaccctaaag agaaagctcg gatcctatat
     5101 caactcaagt aaaactctcc ggtaaatgag cgaaaaaaaa cttatatgga aatcaaaaaa
     5161 tcagataagc gaatcaaaga gatattggcc gctcttttaa tgtttgccaa aggagataat
     5221 tctgaacaac tcaaaatttc ggataatcat gatgaaattg attctatcat gatggcagta
     5281 aatagcttgc gggaatcggt aagagaaaag gatttggaaa tggaagaaag gaaaaacaaa
     5341 acaaaagttc ttattgaatt attgcacaaa tactccgccc tggatttttc tgagcgcgct
     5401 gtcattagta aaaatggaga tgagattgac tcaattgcag cagggttaaa tgcagtaata
     5461 gatgaatggc agtctaaact tgaaaaaacc gaagaaagtg tagaaaaaat aagaacaatt
     5521 ttcaagcatg ctcccgacgc aataattgta attaattcaa ggggagttgt tgagcagtgg
     5581 aatcccgcgg cggaagaaat ttttggatgg tccgaggacg aagtgatgag taaaccgatt
     5641 cataccatta ttattccgga gaattaccgg gaaaaacacc tcaatggtat gaatcgtttc
     5701 ttaaagactg gaaagagttc cgttacaaaa cttcagctta aatcattacg aaaaaataat
     5761 gaaatgattg atgttgaatt aacgatttct tccgctatgg taaagggtga atacttgttt
     5821 attggatttc taagagatat ttctaaaaaa ataaaagctg aacaggaaat cggcaaacgt
     5881 accattcaat tagaaactgc caataaagaa cttgaagaat ttgcctatac tgtttctcat
     5941 gatttaagag gccctctgcg gacattagaa ggcttttcaa aattgctatt tgataaatac
     6001 aacgaaaggc ttgattcaga tgcgacaagg tggctgaatt ttattaataa gaatgcatta
     6061 aaaatggatt tgattatcaa tgatcttctg gagttttcaa taattagtcg ccaggaaatc
     6121 atattagatc gatttgatac aaaatctctt atcgatggag taatgaaaga gtgtttatct
     6181 tcttataaga aaataaaagt tgaacctaaa gtagatattg aatttccaac aacgtatgga
     6241 gatcgaagtt ctttaagaaa agtttggcaa catttaatta acaacgcgct caaattttct
     6301 tcaaagaaag atgtagtaaa attagagatt caggtcaaaa gggcgggaaa cgctataata
     6361 tatagtattg aagacaatgg aacgggattc aatatggagc acagcgacaa aatttttgga
     6421 gtatttgaaa ggctccataa agaatcagag tttgagggaa acggaatcgg gctggctata
     6481 gttagtaaga ttatagaaaa acataacggt aaaatttgga tagaaagtaa agtcgggggt
     6541 ggtactaaat tttattttga actggaattt caggacgtgc caataaagtc ttgagatctg
     6601 gttaaataac tgaacccttt gggttgcaca gcaccctttt gaagtatttg cctaaaattc
     6661 ctgactaaag gtatctgggc gtctggaaca atttcgtgtg atgtttgttg aatgataata
     6721 caagggaaac ttcgtgtgaa tcgggccgaa cataaacatc atctgaaaaa atgtgctgaa
     6781 aggaataatt tatttgtatg gtctgactaa tgttcatacc aaaaataaaa tttgcctctt
     6841 tcccgctatg ataatttata cctgctgaga tcttgtcgta gaaagtggta gtggaaccaa
     6901 atcctacctt tcgggtgaac ccggtttcct tataggagat tgaaggctct gtaataatgt
     6961 gttgagataa agcaagctga taatagcctt ccgcaaaaaa ctgtctcgaa atcttaaaaa
     7021 aatcatcatt attgcgtgaa tacaagagat gtttcaatga cgtggaaact gtgagcttgc
     7081 ccaaatggat ggcaaatcca aaattgaaat cgggagttga ctcattctga tcagattcaa
     7141 tggcattagg gtcaggattt tggtagcgta gttttgaccc atccaattta cggttagcta
     7201 ccccggctcc aagcccgaga gagatcatga aaccgccgaa attaatggat tgtgaataat
     7261 gaagactgac agaagtaatc ttctcaaaac ccagctgatc gtttgacaaa taaaaaccaa
     7321 tgcctttatt ccctctaaac ttattttcaa cactaaataa ttgagtggag gggctgttat
     7381 ccattgcagt ccactggctt ctatgcagca atttaacctg caagtcagca gggttagaag
     7441 taatctgtgc cggattaatc catccacgag tcatagtgta ggtggaaatc aaaggctcgg
     7501 tttgtccaaa gctttgtatt gtggataatg ccagtaaaat aataaatgct gtaaatctca
     7561 tgggggttat aattttgcag ttgataattt ccttttccca aatggaactt ctttcccgtt
     7621 tatcagggca attacaaaag cttcatgacc aaaatttctt actttttcca gaaatgattc
     7681 agcttcattt atatcgatga attcaccaat cgtaaaacta taagttccgt taatttcttt
     7741 cttgtaatta atttcatatt caggaaaatt tccctgtaat ttggttagag ccagcgggtt
     7801 aattttttta tctgcggcct ctattttgat tttgtacacc gctgcgttta attcgtcctt
     7861 tattgaatta ccgacaataa gtcctggctt tttagtgtca gaatatgaat ctttaacctc
     7921 ctcataatta gtgattttga atgtcgttct cctgtttatt tcatgctcat attcatccat
     7981 tgccttgctt accaatggta attcctcgcc ccaacccctt gcttgtaatc gttcagcagg
     8041 agtttcctga ttaaggagaa aagtaactac tgactctgcc cttttttggg aaaggttcag
     8101 gttatagtga ttagaacctc ttgagtccgt gtgcgcgtta atttgaacag ccagcatggg
     8161 gttcgctttt aatacccctg ataacttatt caggctggca taagactcct tgcgcagaga
     8221 atactttccg taatcatagt atatgttttc aatgctgatt tcatctttct gaaattggat
     8281 taactcaaat tttaattctg atgtaaacag atctttttca gaagacaatt ccataaaatc
     8341 attcaggtac tgccctttcg aaacctttaa tgaaaatcca tcgcctgatg caataatatc
     8401 ctgcctgttc tcaaactcga attttccatt gctatcggtc agaacactat ccacatatcc
     8461 tcttgcttcc agaattacca gggctccggg taatagtact cctgttcccg cctctgtgac
     8521 ttcaccttta actacaaact cttctgtctg caacaattgg ggcggggtaa tggttttgaa
     8581 ctgataaata tcatcatcac ccaaacctcc ttttcggtta gagacaaaaa atccgctatc
     8641 cggttttatc atgataaggc caaaatcatc ggcagacgaa tttaccggta actttaaaag
     8701 agcaggttca gaattttgag ctgccagatt aaattcatat atgtcaagac cgccatatcc
     8761 aggccgtcca tcagtcgcaa aataaagcag tgagtcatta tacaatgaag ggaatagttc
     8821 gttaccaggc gtatttactt caggtccgag attttcagga tttccccata cggaaccggt
     8881 tcgggcaatt ttccataaat cattacctcc tagtccgccc gggaggtctg aagaaaatat
     8941 cagggttgta ccatccgttg aaatcgcggg atggcctgaa ttgtatttcg aatttattgc
     9001 ctcaagtaag attccttttg accatgtatc tgaattcaca ttgtattttg agtaataaat
     9061 attgcaggct tttccattgc cttcataacc gttacactgc gtataaaaac cgtagccaag
     9121 aacggtcgag taaaccaatg tgccgtcatt aaactctgaa ttcaattctc cctgcaccaa
     9181 ttccggggag ccgccattgg ctgcatatat attcgaataa ccctgaccat tgaaatcata
     9241 aatagcggtg gaattactat taattctgct ggaactaata aagacttttc catcaagata
     9301 cgaaacgcca taatcactat accgggaatt caatttctta acattggaaa cagcatataa
     9361 tgtatcctct gatgttattg caagtccatt atcggcggta atcagtaaat gttgaaattc
     9421 agggttaaat ttgacattag atctcaacaa atattcacgg gcctcgccgt actttgaatt
     9481 acttattagc agctttgcat atactgcaag gtcaacgtat ttgtacccta ctctgataag
     9541 ctttgtatac cacttcagag aagtttcgta atcgctaaca gcacggtatg actcagccag
     9601 gtaataaata atttcctgct ctttttgtgt ataattactt cctttcggaa ctaattcttt
     9661 gaattgctga atagccag
//
LOCUS       DASWHX010000013         2916 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_2964694, whole genome shotgun sequence.
ACCESSION   DASWHX010000013 DASWHX010000000
VERSION     DASWHX010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 2916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2916
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2964694"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2399)
                     /locus_tag="VGA21_00585"
     CDS             complement(<1..2399)
                     /locus_tag="VGA21_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009578825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HEX9649027.1"
                     /translation="MFSELRQSFKGEILDDFKIRSIYATDASAYRELPLAVVYPKDEA
                     DIQILVRFAGRHKVGLIPRTAGTSLAGQVVGDGIVVDVSRHFTKIIEVNEAENWVRVQ
                     PGVIRDELNMFLEPSDLFFGPETSTANRAMIGGMIGNNSSGSNSIVYRTMRENLISVR
                     GVMSNGSIVEFGEKTAEEFIQKTKDETLEGRVYHSINKILSDSETRQNITREFPKPSI
                     FRRNTGYAIDLLMDSDPFLQNKKPFNFCKIIAGSEGTLLFVTEAKLKVNPMPPAAIGL
                     VCAHFTTIYDCLRANLIALKYKPSASEVMDNYILDCTKGNIGQRPNRFFVQGDPKAIL
                     VIEIRKDTLEEAMKIVNEMVEELKKEGLGYHHPIITGADTSKVWELRKAGLGLLSNMP
                     GDAKPVPVVEDTAVDVNDLPEYIKEFDETMEKQGLPCVHYAHASAGELHLRPILNLKT
                     EKDTKLFRKVLEDVSSLVKKYRGSLSGEHGDGRLRGEFIPFMIGEKNYEVLKQIKEVF
                     DPENIFNPGKIVDTPPMDKKLRFFPGQQTREFDTKLNFDSTHGYLRAAEQCNGAGDCR
                     KTELTGGVMCPSYMATRDEKDTTRARANILREILTNSEKKNPFDSKEIREVLDLCISC
                     KGCKSECPSNVDMAKLKAEFEYQYYKSNKPSARTRRISSFKNQMELATRFRVLYNLLL
                     ASKFPGRIIRNLLGIHPNRSMPKLSGTFRKKFRQTEQGLANEAKRKVYFFCDEFTNYH
                     DPEIGIAAVTLLTRLGYEVELANLTESGRSYISKGFLDEARKIAIENVSKASEFVNPE
                     NP"
     gene            2582..2655
                     /locus_tag="VGA21_00590"
     tRNA            2582..2655
                     /locus_tag="VGA21_00590"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2616..2618,aa:Ala,seq:ggc)
     gene            complement(2665..>2916)
                     /locus_tag="VGA21_00595"
     CDS             complement(2665..>2916)
                     /locus_tag="VGA21_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017730250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HEX9649028.1"
                     /translation="PRTSEGQAKIGEKVKINKIQPGDLVFFATGKRRRKITHVGMVTE
                     VRGSSNVKFIHSSSSLGVVESSLYQEYYRKRFRMARRLF"
BASE COUNT          762 a          661 c          549 g          944 t
ORIGIN      
        1 ggattttcgg gattaacaaa ctcagatgct ttcgaaacat tttcaatggc aattttcctt
       61 gcttcatcca aaaatccttt tgaaatataa cttcgcccac tttcagtcaa atttgccagt
      121 tctacctcat aacctaagcg agtcaatagc gtcacggccg cgatcccaat ttccgggtca
      181 tgataattgg tgaattcatc gcagaaaaaa tatacttttc ttttcgcttc attcgccaac
      241 ccctgctctg tttgtctgaa cttcttgcga aatgttcccg agagtttggg cattgaacga
      301 ttggggtgaa tccccaataa attcctgatg attcgacctg gaaatttact tgccaggagc
      361 aggttgtata aaacccggaa cctggtggct aattccattt gatttttgaa agatgaaatt
      421 ctcctggttc ttgccgatgg tttgttcgac ttataatatt ggtattcaaa ttcggccttc
      481 aattttgcca tgtccacatt agatgggcac tcagacttac aacctttgca tgatatgcaa
      541 aggtccagca cttcgcgaat ctctttgctg tcaaatggat ttttcttttc actgtttgtg
      601 agaatttccc gcaaaatatt tgctcttgcc cgcgtagtat ctttttcatc acgggtggcc
      661 atgtaactgg ggcacattac tccgccggtt aattcagttt ttcgacaatc cccggcgcca
      721 ttgcattgtt ctgcagcccg caggtatccg tgggttgaat caaaatttaa tttagtatca
      781 aactcacgcg tttgctgtcc agggaaaaac ctgagtttct tatccattgg tggtgtatcc
      841 acgattttcc cgggattaaa aatattttcg ggatcaaata cttcttttat ttgtttcaac
      901 acttcataat tcttctcacc aatcataaac ggaataaatt caccacggag ccggccatcg
      961 ccgtgctctc cacttagtga cccacggtat ttttttacca gggaagaaac atcttccagg
     1021 actttgcgga atagcttcgt atctttttca gtttttagat taagaatggg tcttaaatgc
     1081 aactcacccg cagaggcatg agcataatga acacatggca acccctgctt ctccattgtt
     1141 tcatcaaact cttttatgta ttccggaagg tcgtttacat ctacagctgt atcttccacc
     1201 acaggtaccg gttttgcatc tccaggcata ttggataata accccagacc cgcttttcgc
     1261 agttcccaaa ctttagaagt atctgcaccg gtgataatcg gatgatgata tcccagtcct
     1321 tcctttttca gctcttcaac catttcattg acaattttca tggcctcttc cagcgtgtct
     1381 tttcttattt caatcacaag aattgctttg ggatctcctt gcacaaaaaa tcgattggga
     1441 cgctggccaa tattaccttt tgtgcaatcg agaatgtaat tatccattac ctcggatgca
     1501 cttggtttat atttcagagc aatcaggttt gcacggagac aatcgtatat tgtagtaaag
     1561 tgagcacaaa ccaatccaat cgctgcgggt ggcatgggat ttacttttaa tttagcttca
     1621 gttacaaaaa gcaaagtacc ttcagagccg gcgattattt tacagaaatt aaaaggtttt
     1681 ttattttgaa ggaaagggtc tgaatccatg agaagatcta ttgcataccc tgtatttctc
     1741 cgaaaaatag atggctttgg aaattcacga gtgatgtttt gcctggtttc tgaatcagag
     1801 agaattttgt ttattgaatg atagactctt ccttcaagag tttcatcttt tgttttttga
     1861 atgaattctt ctgcagtttt ttctccaaat tcaacgatgg aaccattgct cattacaccg
     1921 cggacggaaa tcaaattttc ccgcatagtt ctataaacga tagaattgga gccgctggag
     1981 ttgttcccaa tcatgccacc gatcattgcc ctgttagcgg ttgatgtttc cggaccaaaa
     2041 aacagatcac ttggttccag gaacatgttc aattcatccc ggataactcc cggctgaacg
     2101 cgcacccagt tttcagcttc attaacttca atgatttttg tgaaatgtct gctcacgtct
     2161 acgactattc catctcctac cacctgaccc gccagcgaag ttcctgcagt tctgggaatt
     2221 aatcctacct tatgcctgcc ggcaaacctt accaggatct ggatatcggc ttcgtcttta
     2281 ggatacacta ctgcaagtgg aagttcccgg taggcagagg catccgttgc ataaatagaa
     2341 cggattttga aatcatccag gatttcacct ttgaagcttt ggcgcaattc gctaaacatt
     2401 aattctataa aattacaata tctctattta tcctgagacg ataaatagaa agggaagaaa
     2461 atgaataatt gcttttttta taaattattt ttatcaagca atcaatgaag ttggggatct
     2521 accttcatga ttaaaatcga tttctgataa taggaggata tgcctatatt tgccctcttc
     2581 cggggcatta gctcagctgg ctagagcgct acaatggcat tgtagaggtc atcggttcga
     2641 ctccgatatg ctccactact attcttaaaa gagtcttcgc gccatcctga acctcttcct
     2701 gtaatattcc tgatacaggc tgctttctac cacgccaagg ctggacgaag aatgaataaa
     2761 ttttacgttg gaggatcccc gcacttccgt taccatccca acatgtgtaa ttttacgtct
     2821 tctttttccc gtggcaaaga acaccagatc tccgggttga attttattta ttttcacctt
     2881 ttcaccaatt ttggcttgtc cttcggaagt tcttgg
//
LOCUS       DASWHX010000014         3010 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_2964868, whole genome shotgun sequence.
ACCESSION   DASWHX010000014 DASWHX010000000
VERSION     DASWHX010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 3010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3010
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2964868"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..636
                     /locus_tag="VGA21_00600"
     CDS             <1..636
                     /locus_tag="VGA21_00600"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX9649029.1"
                     /translation="LDVIGVVEDFNVQSLREPVQPLVHYYGGDSARSYRFVSVKINPD
                     SREQILAEVARTWQRMDFGQELEYYFPAERFRELYATEENVASVLTFSMSFAILIACL
                     GLYALASFSVALKSKEIAIRKVMGATVQSIITAFSRKYLLLVGVAGIPAIVLSWYGMN
                     MWLEDFAYRITIGADIYLLTILLVILIAFATIAWHALKAGRTNPVDYLREQ"
     gene            complement(756..1313)
                     /locus_tag="VGA21_00605"
     CDS             complement(756..1313)
                     /locus_tag="VGA21_00605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649030.1"
                     /translation="MRYLPILLLFFLSACKCKDGLTFLLDEEFKSYTDFPEGSWWVYE
                     KVGDPSVTDSIYLYRRSERLAPPRGSCDEYQLFFYYLVRHQAKDIKEFSFVSEHYTDS
                     TNYLRSGLWYGTQFLFTKKKGLDPLAQVETGIINGIVFDSTITFNHRSSYYFLSETYA
                     RRIGVVRRLYQDSSLYEIKKYFINH"
     gene            complement(1605..2129)
                     /gene="idi"
                     /locus_tag="VGA21_00610"
     CDS             complement(1605..2129)
                     /gene="idi"
                     /locus_tag="VGA21_00610"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013409566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HEX9649031.1"
                     /translation="MEEVILVDKKDQVVGSMEKLEAHKKGVLHRAFSVFIFNSAGQLL
                     LQKRALKKYHSGGLWSNTCCSHPRPGEDTRDAAARRLQEEMGLTSRLKKAFSFQYKVK
                     FENGLTENEFDHVFVGLCEVDPNPDPREVADWKFIDMDFVLRDSVNQPEQYTYWFRKS
                     ISKVLDYLHRNPVK"
     gene            2372..>3010
                     /locus_tag="VGA21_00615"
     CDS             2372..>3010
                     /locus_tag="VGA21_00615"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9649032.1"
                     /translation="MVRNYFKVAIRNLWRQKGFAFINVFGLSVGLACCILIFLYVKEE
                     ITFDTFHNKSERIYRAWVLEDYGDGKVFFNTVTPGVLAPTLSSHYNEISKVTRFSNRG
                     GLIRKDGQMFNELIWQAGPEFFDIFDFPVLSGSTEKSLEELNSIVITEKAARKYFGSD
                     PAVGKVMEIQMGAEFQNFFVKAVLSDIPANSSLQFDYLISDLHYKTLYNPRTV"
BASE COUNT          900 a          561 c          596 g          953 t
ORIGIN      
        1 ctggatgtga ttggtgtggt agaagatttt aatgtgcagt cacttagaga acccgtgcag
       61 ccgcttgttc attattatgg aggtgacagc gccagatcat acaggtttgt ttctgtcaaa
      121 atcaatcctg attcgcggga acagatattg gcggaagttg ccagaacctg gcagcgaatg
      181 gatttcggtc aggaattaga atattacttt cctgcagaaa gattcaggga actttatgca
      241 acggaagaaa atgttgccag cgtacttacc ttttccatgt cgtttgcaat cctgattgcc
      301 tgccttgggc tttacgcgct ggcatcattt agtgtggctt tgaaatcaaa agaaatcgcg
      361 attagaaaag tgatgggcgc caccgttcaa tccattatta ccgcattttc acggaaatat
      421 ttgctactgg tgggagtagc cggaattccg gcaattgttc tctcctggta tggaatgaat
      481 atgtggcttg aagactttgc ctatcgaatt acaattggag ccgatattta cctgttaacg
      541 atactgcttg taatcctgat tgcgtttgct accattgcct ggcatgcttt gaaagcaggc
      601 cgtacaaatc cggtagatta tttgcgggaa cagtaatctg gaaggtagtt atcatttaga
      661 attttttcag gcaaacttat ttctgccagt gagttctcct tctttataaa attacaacaa
      721 aataaaagaa tagctttttc atagaagttc acagtttaat ggttaataaa atattttttg
      781 atttcatata gtgaggaatc ctggtacaag cggcgcacaa ctccaattct ccttgcgtat
      841 gtttcactca aaaaataata agaagaacga tggttaaatg ttatcgttga atcaaataca
      901 atcccattaa ttatcccagt ttcaacttgg gcaagtggat ccaggccttt ctttttggta
      961 aaaagaaact gtgtaccata ccaaagacca gatcgtaaat agttagtgga gtcagtgtaa
     1021 tgttcagaaa caaaagaaaa ttctttgata tcttttgcct gatgtcgaac caagtaataa
     1081 aagaaaagct gatattcatc gcacgaacct cggggcggtg ctaacctttc acttctccgg
     1141 tacaaataaa tactatcagt aacggaagga tcgcctactt tttcatagac ccaccagctt
     1201 ccttccggga agtctgtgta cgatttaaat tcctcatcca ggagaaaggt caagccatct
     1261 ttgcatttgc aggcagagag gaagaataac aataaaattg gaagatatct catgctaatt
     1321 aggtaccata ttgtataaat atagttataa tttatgaatt tttatcaaat atctaagaat
     1381 tgcatgttaa gtataagttt gcataagggg taagttagga tctgtctccc tttggactaa
     1441 ctaaccctta aaatgcattg tgtattccgg cagttacatt ttcaaaataa atggaagagc
     1501 gcggagaaga aggaataaca attttataga atttgataat tgaagaaact cattaagcca
     1561 atccgtcatc cagattggtc cattttaatc ttgggagaag cacttcattt aactggattc
     1621 ctgtgcaggt aatccagaac ttttgatata ctttttctaa accaataggt gtattgttca
     1681 ggttggttaa ctgaatctct gagaacaaaa tccatatcta taaatttcca gtcagctacc
     1741 tctcgtggat ccgggttggg atctacctcg cacaatccta cgaagacatg atcaaactcg
     1801 ttttcagtta aaccattttc aaatttaact ttgtattgga atgaaaaggc ttttttcaat
     1861 cgtgaggtca aacccatttc ctcctgcaac cgtctggcgg cagcatcccg cgtatcttca
     1921 ccaggtcggg ggtgactaca acaagtgtta gaccatagcc caccgctgtg gtattttttt
     1981 aatgcgcgtt tttgaagtaa cagttgacct gcagaattaa aaatgaaaac cgaaaatgcc
     2041 cggtgtagca ctcctttctt gtgggcttcc agtttctcca tcgaacctac aacctgatcc
     2101 tttttgtcaa ccagtattac ttcttccatt caaaattttt tgacgaattt acccaatcct
     2161 attgaagaac tcaatttgga atgcctttaa ttgcataaaa tacccgggtt tattgaagtt
     2221 tgaacacact tgttttaatt ggaaattggc attttacaac aaagaacttg gggttcaatc
     2281 atcaaatcgg tcaatacagg ttagaatatt ttagcacgca aattttttat tttagatttg
     2341 aataaattag cggaacttaa tttttgtaac tatggttaga aactatttca aagtagcaat
     2401 acgtaaccta tggcgccaaa aaggctttgc atttattaat gtatttggat tatcggttgg
     2461 cctggcatgt tgtattctca tatttcttta tgtgaaagag gaaattactt ttgatacttt
     2521 ccacaacaaa tctgaacgta tctaccgtgc ctgggtgctg gaagattacg gtgacggaaa
     2581 agtgttcttt aataccgtta ctcccggggt tcttgctccg acactatcca gtcattataa
     2641 tgaaattagt aaagttaccc gcttctccaa caggggtgga cttatcagaa aagacggtca
     2701 gatgtttaat gagctgattt ggcaggccgg gcctgaattc ttcgatattt ttgactttcc
     2761 tgttctttcc ggttccacag aaaaatcgct tgaagaactt aattcgattg ttataactga
     2821 aaaagcagcc cgaaaatatt tcggaagtga tccggctgtt ggtaaggtga tggaaattca
     2881 aatgggagca gaatttcaga atttctttgt gaaagcagtt ctcagtgata ttcctgccaa
     2941 ttcaagtttg caatttgatt atctaatttc agacctgcat tataaaacgc tatataatcc
     3001 aagaactgta
//
LOCUS       DASWHX010000015        79522 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_8553, whole genome shotgun sequence.
ACCESSION   DASWHX010000015 DASWHX010000000
VERSION     DASWHX010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 79522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 79522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..79522
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8553"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1104
                     /locus_tag="VGA21_00620"
     CDS             <1..1104
                     /locus_tag="VGA21_00620"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009183577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEX9649033.1"
                     /translation="LGGGQLGRMMIQAGIDFNLDIHVLDPDPDAPCKSLASTFTKGSF
                     TDELTVYNFGKDKDLVTIEIENVSVSGLKRLELEGKKVFPQPQIIELIQDKVKQKIFY
                     RTHNIPTAEFRIVKSKAELAKHSEIIPAVNKLAKSGYDGRGVKVIRTAEDLNAGFDDY
                     GLLERFVNFKKEISVIVARNEKNQIKTYPPVELVFHSDANLVEYLFSPAEVSREVEIL
                     SKKIAIDLAEKLNLVGLLAVEMFVTEDDSILVNEIAPRTHNSGHQSIEGNQTSQFEQH
                     LRAILNLPLGSTKVIQPSAMVNLLGEEGYSGLAMYEGLMDVLSVSGVHIHLYGKKITK
                     PFRKMGHVTITDKDLNKLREKAEFVKSTLKVKA"
     gene            1101..1616
                     /gene="purE"
                     /locus_tag="VGA21_00625"
     CDS             1101..1616
                     /gene="purE"
                     /locus_tag="VGA21_00625"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009197609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEX9649034.1"
                     /translation="MKKPMVGIIMGSDSDLTIMQEAAAILKKLSVPYEITIVSAHRTP
                     KRMVEYAETARKRGLKVIIAGAGGAAHLPGMVASITTLPVIGVPVKSGNSIDGWDSIL
                     SILQMPGGVPVATVALNGSKNAGILAAQIIGTNSETVRKSLEKLKHEMAVSVEQKAAR
                     IEMKTAVRKKK"
     gene            1834..3120
                     /locus_tag="VGA21_00630"
     CDS             1834..3120
                     /locus_tag="VGA21_00630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649035.1"
                     /translation="MLNPGQKGWLKEYLEFRKSILDSKTENSLHKKPAPRHPEESLYG
                     ALQPTGLMYGHPIKFSQLDEKLMEEWGSHNVMKVLLAESLINSSLVQHETEIHNEDEF
                     YEVVLKTISNITGFYNKVYPELSTSERTFLGKKKPPLEVAEKILEKRIGLTHESSKNF
                     WVNFFHNSLLFLDIYFFGQWIHTNSEEAVTEFFRHEKEDLRFSVIRVIVAAAHANQVI
                     EKEEQKLFEFFLDSSDLTSEKKKEAHKFLEEGVDLSEIEVPGNSWILRKYFLELAILT
                     VWADKKVEDSEVEFLENLTRKLGLSEEDLENSMIAIEGFVIEYWDELGQLQSKHDLTQ
                     VSEKYLERISKVVAHNKNRIANEINESKQLKILLDKYLRGEISGSDAEKLHMELLAIV
                     KTIPTFVIISLPTTFLSLPILIKILPKSVFPGNENS"
     gene            complement(3115..3699)
                     /locus_tag="VGA21_00635"
     CDS             complement(3115..3699)
                     /locus_tag="VGA21_00635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649036.1"
                     /translation="MRKILLVSLCLFSCSQAEQEVKTGELYFSLEKLVEEQVSILSSG
                     TYQIKKTAFLNEKHDEKILKPDSLGWLDELKLFRDADINLPSLRGVYEMVAGPGELTS
                     NLQEIDFIPVKEVQGVQYIKVFYAENRDDVRKIETRVTEVNSLFRGETILKLELRRDG
                     ENNLVLTSYEITGGQKMIMLDSVEYKITGLVTRL"
     gene            complement(3696..5222)
                     /gene="gpmI"
                     /locus_tag="VGA21_00640"
     CDS             complement(3696..5222)
                     /gene="gpmI"
                     /locus_tag="VGA21_00640"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013454266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HEX9649037.1"
                     /translation="MARKIILIILDGWGIAKNTKVSAIDQANTPFIDSLYETCPHSTL
                     RTSGLDVGLPDGQMGNSEVGHLNIGAGRIVYQDLVKVSKAIEDGTVENHEVLNDAFDY
                     AKANDKKVHLFGLVSDGGVHSHIDHLKGLMTIAAKKGIKNLFIHAFTDGRDTDPKSGI
                     GFITQIIKHGRKTSGRLASVVGRYYAMDRDKRWERVKLAYDCLVFGKGNPTTDVIAAI
                     NDSYSKNITDEFLKPLVLTDDKSAPVATIQEGDVVICFNFRTDRGRQITQVLTQFDLL
                     DFEMKKLSLYYVTLTNYDNTFQNVKVIFDKDNLEQTLGEVLSERELHQIRVAETEKYP
                     HVTYFFSGGREKEFKGEKRILCPSPKVATYDLKPEMSAEDVRDRIVAELRTQWADFVC
                     LNFANADMVGHTGVFEAAIKACETVDRCTKDVVETGLENGYSSIIIADHGNSDYMINE
                     DGTPNTAHTTNPVPCILVDKSFNGKIKNGRLADVAPTILQLMDIPKPSLMTGESLIDL
                     "
     gene            5386..5790
                     /locus_tag="VGA21_00645"
     CDS             5386..5790
                     /locus_tag="VGA21_00645"
                     /inference="COORDINATES: protein motif:HMM:NF027349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4783 domain-containing protein"
                     /protein_id="HEX9649038.1"
                     /translation="MKKEPLYRLFFLVIFFVFGFVGKAQEDIIHSVESAMKLGSSKEL
                     ARYLNQTVEINLEGEISSYSKMQAEFVLKDFFKKNPPEGFEIIHNGTSKGGLKYAIGK
                     YSCKRGYFRVWMRVKEFSGKLLVYEMNFFKED"
     gene            5834..6691
                     /gene="nadC"
                     /locus_tag="VGA21_00650"
     CDS             5834..6691
                     /gene="nadC"
                     /locus_tag="VGA21_00650"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018476294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEX9649039.1"
                     /translation="MNLHYLTNQALNDFIELALAEDIGDGDHSSLAVLNHDLEQSAQL
                     IIKGEGILAGVELAERILLFKNPKVELIRNSGDGDPVKNGDIAFTLSGNARKILSHER
                     LVLNCMQRMSGIATYTSMLQSLISHTKAKVTDTRKTTPNFRLMEKWAVAIGGGVNHRM
                     GLYDKILLKDNHIDYCGSMANALNKVKDYLEKTGKDLFVEVEARNLDEVTQALKFEIV
                     STIMLDNMPPSEIIRAVKITDGKVKTEASGNITESTIKEVAECGVDYISVGALTHSYK
                     SLDMSLKAF"
     gene            6736..7209
                     /locus_tag="VGA21_00655"
     CDS             6736..7209
                     /locus_tag="VGA21_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009183564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcxB family protein"
                     /protein_id="HEX9649040.1"
                     /translation="MIVKTRKYQIPKKTYIKLGLRNIMKEQWWVLLIVAAICSGAFLV
                     PSNWWFIGAAIGLVLYFLFWLIQFAGMSQLEQGKILFDKLSYEIDSRQILIKINTKQG
                     MPIKWEQIKRAWMSKDAFTFVISKVQFIHLPFRIFNAENQVKFIETILKRKGYLK"
     gene            7215..7688
                     /locus_tag="VGA21_00660"
     CDS             7215..7688
                     /locus_tag="VGA21_00660"
                     /inference="COORDINATES: protein motif:HMM:NF014668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HEX9649041.1"
                     /translation="MGKGEKLTESELRLRISSYCAYQERSPKQVQEKLSTLNTDAIIN
                     ERVIKWATDEKFVSESRFAKNYVHGKFHLKKWGRYKIKAGLKAHDISTQIIGKAIAQL
                     DNTNYVETLITLGIKKFQSIKKTDQNKWRKTENYLIGKGFETKLILENKEKIENG"
     gene            7681..8130
                     /locus_tag="VGA21_00665"
     CDS             7681..8130
                     /locus_tag="VGA21_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019943601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing (seleno)protein"
                     /protein_id="HEX9649042.1"
                     /translation="MGKILIILMIACVNPWQTVKTLNLNLEDGVAIQGYDPVSYFSKG
                     GPKEGKPGIRYVHNDATYYFLSDENKSKFVANPTQFEPKFGGWCAYAMGDSGEKVKID
                     PETFKIVDGKLYLFYNFFFNNTLKDWNENEKVLKQKADENWSKITRN"
     gene            8280..8795
                     /locus_tag="VGA21_00670"
     CDS             8280..8795
                     /locus_tag="VGA21_00670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX9649043.1"
                     /translation="MELQNALKKVLGQLRDVTGQLKETEFIMPVKSLNGSTIGQHTRH
                     TIEFFTCLKDGYKDGRVNYDKRSHDTEIESSKVLAMEMIARLEDFVMFLPADKPFVLE
                     VNYDPDSGDNVLIHSSYYRELVYNIEHAIHHMALIKVGLKEICSAVKIPSDFGVAVST
                     VKYQKIQTLKA"
     gene            8886..9869
                     /locus_tag="VGA21_00675"
     CDS             8886..9869
                     /locus_tag="VGA21_00675"
                     /inference="COORDINATES: protein motif:HMM:NF020032.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649044.1"
                     /translation="MEIVYIPVMFYWLWLSIKARSPFFFSASNPGIEFGGMLGESKIK
                     ILKKIDDELKPKTLYFNQNVSIEEVLNKIEDASLSFPLIFKPDIGERGLMVEKIDTEA
                     AATNYVDSIKMNFLVQEYIDLPIELGVFYFRYPNRSAGRISSIVIKEFLKITGDGKST
                     IRNLIRKNPRAKLQLDVLEHKYGAEMESILPMAKEKLLVPIGNHCKGAAFLDGGKRIT
                     KKMTRSFDKIAKEIDGFFIGRFDLKCESWEDLEDGNMKIMELNGSGAEPGHIYHPGAS
                     IWNAYESLFFHMRVLLRISMINKKSGVKYLTWKEGWYVFTQLPTFKKLGFT"
     gene            9873..10496
                     /locus_tag="VGA21_00680"
     CDS             9873..10496
                     /locus_tag="VGA21_00680"
                     /inference="COORDINATES: protein motif:HMM:NF013933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HEX9649045.1"
                     /translation="MEAITLFTIGLFFSYIGSIPPGSINISVLQLSMGGHRAAAVRFT
                     LVAAVIEFVYAYIAVKFQQLILSSQMLQDNFKLIAAVVLIILGLLSLRSGSGQKRWEK
                     KLESSGLRKGLMVSVANPLAIPFWIAITAYLETNNWISLNSSNEFIYIAGIAAGTIAL
                     LFTLVFLGSKASRFMTNQERAKKIPGFVLLGLGIYSLVQLLIEVVTR"
     gene            complement(10454..11644)
                     /locus_tag="VGA21_00685"
     CDS             complement(10454..11644)
                     /locus_tag="VGA21_00685"
                     /inference="COORDINATES: protein motif:HMM:NF024779.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4105 domain-containing protein"
                     /protein_id="HEX9649046.1"
                     /translation="MKNYLIPLLFTLSVPSLGQARLSDQSKVFMLTFGPGQQQVYTCF
                     GHSAIRITDASKGIDLVYNYGTFDFDQKNFYLNFAIGEPYYSLAIGKYEHTEYVYMYF
                     NQSIYQQELNLTLSQRQTLFEFLNWNSLPENKDYLYDYFYDNCSTRIPDAFFHVFGDR
                     IYLDTITYVTEARSFRDMVNVYTPLQPWGDLGIDLGLAIEIDKIMQPGDYLFLPEYLY
                     EAVDNGTILHGDSIVPLVKDKRVIFEAKPVEYNLGFFTPKTVFWLLLLVVAGFTLWEI
                     LKRRHLKLIDVILFSPVWLLGIFLVILWFGTEHKSAWNFNLLWANPFHGIFLYFLIKN
                     KKPKLIVYHFLGYGILLLLTLVFWRVLPQALHPAFVPLVIALALRSFWVYRVTTSIRS
                     WTSE"
     gene            11722..12741
                     /locus_tag="VGA21_00690"
     CDS             11722..12741
                     /locus_tag="VGA21_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018343491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HEX9649047.1"
                     /translation="MRSFSDRLNLLSKTSPGKLINASRVLSSYYISRISGMPNHRGLP
                     FSIGIEPTTSCNLRCPECPSGLRSFSRPTGNLSSDTFTKVIDQLSTHLIYLTFYFQGE
                     PYLHPKFLEMVSYASGKGIYTSTSTNAHYLDETTARRTVESGLDRIIISIDGADQETY
                     QQYRVGGSFERVINGTKELLSARKKSGKMKPHVAFQFLVVKPNEHQVTGINRLGENLG
                     VDEILFKTAQIYDFENGSPLIPDQSKFSRYHKTKNGNWEIKNSLENHCWKMWHSCVIT
                     WDGAVVPCCFDKDASHSMGNINEMPFKEIWEGEKYRKFRQSLLSSRKEIDICKNCTEG
                     TTVWV"
     gene            complement(12738..13523)
                     /locus_tag="VGA21_00695"
     CDS             complement(12738..13523)
                     /locus_tag="VGA21_00695"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018472601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEX9649048.1"
                     /translation="MEINIEKEVIELVKEVGEFILKERETFSYEAVELKSFNNLVSYV
                     DKQAELKLIEGLGKLAPEAGFLVEEGTVPQSESEWQWIIDPLDGTTNFVHNIPAYSIS
                     VALAFEEEVYLGVVYEITRKECFYAIKDKGAFLNGKPIQVSASKLLEESLIATGFPYH
                     DFGKTGTYFEILEEMMRKTHGVRRIGSAAVDLAYTACGRFEAFFEYNLNPWDVAAGTL
                     LVKEAGGTVSDFSGGNSFLFGREIIAAGNVYPLVLEIIKNKWI"
     gene            complement(13513..14337)
                     /locus_tag="VGA21_00700"
     CDS             complement(13513..14337)
                     /locus_tag="VGA21_00700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649049.1"
                     /translation="MSAGTDKLDYRYLLKLSGAAVILQTLAWTISPFPFFQFFAYVPI
                     IAACLYLNEVKIKFYLELLIIIFFTFFIPALFKFLVFTPSLRDFVASLSPALTFLLWF
                     YSGTRLGRLKSFYLVIFWLGVEYISIAISPPVYNHLPGAAFRLWGESMVWIRFTGFQG
                     TTFWLLLTNILFTHSFFDEKGKFLSKIIRTRYLISSGVAILPMIISMFVFKGNPEGDL
                     TGFFENPSVYWNNFRLNPFYSYYLDYGEYLGKTSFWLSIFILLSLMVKGYLSKNGN"
     gene            complement(14318..15382)
                     /locus_tag="VGA21_00705"
     CDS             complement(14318..15382)
                     /locus_tag="VGA21_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009579437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9649050.1"
                     /translation="MKTILLIEDNQDVLENTAEILELANYKVMTANNGRAGVELAQKN
                     MPDLIVCDIMMPELDGYGVLHILSKNEKTAGIPFIFLTAKADRADLRKGMNLGADDYL
                     TKPFDDTELLDSIESRLRKSDLMKKNFQRSVEGINEFIQDAATVSNIDMLISNHKLRM
                     FKKKASVFHETDYPNHLYFISSGRVKTFKSNQDGKDLILGVHSTGEFLGYNALITNTS
                     YSESAEALEDSELYVIPKQDFESLMYTNRDVTRKFIQILSDNLQEKEEQMLHLAYNSV
                     RQRVAEALINLEEKYNSTTPGETLTISREDLANIVGTSPESVIRTLSDFKDEKSIEIK
                     SGKIRILDPGKLEKIVSWNR"
     gene            complement(15360..16670)
                     /locus_tag="VGA21_00710"
     CDS             complement(15360..16670)
                     /locus_tag="VGA21_00710"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HEX9649051.1"
                     /translation="MGRKDSYKKLFESDDAFKAIYKSATEGILLANQKGVIADANPAS
                     HSLFGYGSNELIGKSVESLIPTNLRTNHIKMRGTFHKNPTPRVMGAGRDLVGLRKNGT
                     EFPIEVSLSHFEIEGVMFAIAFVIDISVRKNAELELKNSEEKLIVYATELERRVQNRT
                     EQLAESVQNLEKINNELEREIKERKRAEKEALVALEKEKELNEMKSRFVSLASHEFRT
                     PLSTILSSASLIGKYDKEEMNEKRIKHINRIKDNVSEMTGILNDFLSLDKLEAGQVES
                     RHEKIELISFIKEIIAELKTITRRGQIITNSFNVRTYELFIDKSFLKNILNNLISNAI
                     KYSPEGDPIEVSVVTSKSSVTIEVKDYGIGIPEEDQIHLFKRFFRAHNSIHIQGTGLG
                     LNLVKRYIDLLGGSISFKSEVGKGSTFMIKLLNNLTDDENNTTN"
     gene            16781..17632
                     /locus_tag="VGA21_00715"
     CDS             16781..17632
                     /locus_tag="VGA21_00715"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX9649052.1"
                     /translation="MASESQFHKRIVSDILVPVDFSVNSDNALIYAINIARQCNAKLW
                     ILHCYRLIQTVPEMEGVAGFSLKEVLEKAWKDEFEKLKKNYFHEDFTNYSFLLEIGFP
                     EDVIKLQTKKIPIDLVIMGTAGATGIGEILGSTTSKVVDMVDCPVLAIPASARFNGIK
                     KVTIAYDEEKVNDPNSYSMILNLVRKFKSKLDIVNFNTTLKTGGKESEGRLSLDPYLD
                     GVKHKYHFKAAGKIEKAITDFVEAEGTDILVMVAHDHGFIESLFHSSITRKMVLHAKT
                     PLLIIHE"
     gene            17655..18545
                     /locus_tag="VGA21_00720"
     CDS             17655..18545
                     /locus_tag="VGA21_00720"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX9649053.1"
                     /translation="MKNILVPTDFSEYANNAFDFACSIAEHSKGEVTLLHVLEYPVMS
                     VFNTTGEISMDSITDAYIVNLKKRADEELDKKLVNPAYPDVRIKKHIRIGNPYENIAR
                     LIQQSDADLVVMGTKGASGLRELFIGSNAEKAVRFSKCPVITVPGKFDFNAIKKIAYG
                     ITFEDNHNEALFELKKYLHLFKAELHLVWVNTLYDKITDTEARSKMDQLAKLHMLPNY
                     SVNCYKSSAADDGLLFYAEENNIDMIALATHSRKGLAHIFAGSFAEDVVNHSIRPVWT
                     FSLKSENLKAKINEKQKQYQ"
     gene            18520..19386
                     /locus_tag="VGA21_00725"
     CDS             18520..19386
                     /locus_tag="VGA21_00725"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX9649054.1"
                     /translation="MKSKSNISKILVPTDFSPEAINAMTFAAQVARLTQAEVTLLYVL
                     NYPGGSAFDTMMSEYEDPSKNKYVQEVLKDVNEVFQKYEISKTFKDVKVVKKIQIGNP
                     FTNISDEISQNKTDLVVIGTSGLGNARNNNLIGSNTEKVVRRASCPVISVKEDIDLKK
                     LKSIVFPTTFEKESDSMALFLKDFSKMIKAEVKMVFINTPAYFKTQHMIEKAYEKYVT
                     KNKIKDFFFMVYNELTEEKGILHYSADIDADMIALATHGRKGLAHLFAGSIAEDLTRH
                     TKRTLLTLKTWN"
     gene            19420..19830
                     /locus_tag="VGA21_00730"
     CDS             19420..19830
                     /locus_tag="VGA21_00730"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX9649055.1"
                     /translation="MKIQAPVSQIMTPEPIYAEADNTFSQARDLFLKFKIHHLPVVKD
                     GNPIGIISTIDILKAYTVAGQKKWECDDVSMNKKLSISDVMTDDPVNISPATSLQQAI
                     RVFQESNFQALLVVEFGKLVGILTLKDIVSHITD"
     gene            19904..20635
                     /locus_tag="VGA21_00735"
     CDS             19904..20635
                     /locus_tag="VGA21_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009578995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HEX9649056.1"
                     /translation="MERKHSEERLHKVKAGFGKFTNYLREFVYGGMDGSVTTFAVVAG
                     AEGANLGSTVIIILGFANLLADGFAMSVGSYLSSKSDNENYERHKKTEYWEVENIPEA
                     EREEIREIYRQKGFEGELLEDVVRVITADKDRWVNVMMKEELEMIPENKSPFAMGAAT
                     FISFVLVGLIPLMSYVADSFISLNNTFAISAILTTLAFVIIGMVKTKVTQTSIWKGVM
                     ETLILGCSAAVVSYLVGFLLESFVP"
     gene            20808..21278
                     /locus_tag="VGA21_00740"
     CDS             20808..21278
                     /locus_tag="VGA21_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020532850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX9649057.1"
                     /translation="MKKLLLNAALIIAVTFLFGNCGGSGSETENVETTPKPRLAGEDA
                     DPMENKGIGPVKSLNLPDAIDQAMAAEGAIIYVQYCTSCHKPGEKFIGPAPKDVLTRR
                     SPEWIMNMILNPEQMVKEDPIAKKLLVEYNMAPMANQNLTEEQARKILEYFRTL"
     gene            21299..23245
                     /gene="nosZ"
                     /locus_tag="VGA21_00745"
     CDS             21299..23245
                     /gene="nosZ"
                     /locus_tag="VGA21_00745"
                     /EC_number="1.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019598414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050304 - nitrous-oxide reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019333 - denitrification pathway [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-dependent nitrous-oxide reductase"
                     /protein_id="HEX9649058.1"
                     /translation="MKTNQFYAAILITSLSMLFACNGAQKGSGALSSDAASRVYVAPG
                     EYDEFYAFLSGGFSGQVSVYGLPSGRLLKVIPVFSVDAEKGYGFNEETKPMLNTSFGF
                     IPWDDSHHPELSQTNGVPDGRWLFINGNNTPRIARIDLTTFETTDIIEIPNSGGNHSS
                     PFTTMNTDYVVAGTRFSVPIPQEDVAISEYKNKFKGVLSFIKVDDESMDIAFQILVPG
                     FDYDLAHSGKGKSDGWTFFTTYNSEKAHTLKEVNASQNDKDFIAAVNWKKAEEYLAQG
                     KAVDFPTSYVVNRYSEKTHMATSETRTTVKVLLASECPGLIYYLPTPKSPHGVDVDPT
                     GEYIVGNGKLSADLTVHSFDKMMTAINNESYDGEIDGIPVLKFDEIVAGIVQSGGLGP
                     LHTEFDGKGNAYTSFFISSEVVKWKLGTWEVLDRVPSYYSIGHLMIPGGDSMKPWGKY
                     LLSLNKITKDRYLPTGPELNQSAQLIDISGDKMEILLDFPTIGEPHYAQAIAADLVMP
                     NSKKIYNLKENEHPYAVKSEGETKVERKGNEVRVYMTSIRSHFAPDNIEGIMVGDKVY
                     VHLTNLEQDWDTPHGLSIMGANNAELLVMPGQTETLYWEPQRPGVYPFYCTDFCSALH
                     QEMQGYIRVSERGSNTELKWSLGN"
     gene            23301..23867
                     /locus_tag="VGA21_00750"
     CDS             23301..23867
                     /locus_tag="VGA21_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018479207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649059.1"
                     /translation="MKKINLFQLVASFLLFALFIFPLWKITLTAPQYPEGISMYIWIN
                     KITGDTEYTLQNINILNHYVGMKYIVPDEFAEFKYFPIIVAGMAVIGMILSFFKNKKL
                     AIGWLVVLLFMGCLGIYDFYLWEYDYGHNLSPTAPIKVPGMAYQPPLLGSKMLLNFKA
                     DSYPYWGGLFLGITIVLSTFSIFKKNKR"
     gene            23864..24307
                     /locus_tag="VGA21_00755"
     CDS             23864..24307
                     /locus_tag="VGA21_00755"
                     /inference="COORDINATES: protein motif:HMM:NF017394.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase accessory protein NosL"
                     /protein_id="HEX9649060.1"
                     /translation="MKELFLTLCCLAFLSCSSGPQPIAYGTDGCHYCKMTIVDNKFGA
                     EIVTRKGKIFKFDAVECLVNYSKQNPKQDGDTYYVINYLNGEWIVAEGAAYLRSKKIP
                     SPMGQYIASFSAREAAKDIQNENEGVIQTWNELIDNFEITKIENF"
     gene            24333..25565
                     /locus_tag="VGA21_00760"
     CDS             24333..25565
                     /locus_tag="VGA21_00760"
                     /inference="COORDINATES: protein motif:HMM:TIGR04247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase family maturation
                     protein NosD"
                     /protein_id="HEX9649061.1"
                     /translation="MIFLTIAGACKLHASTTEVCSNCQIKTITEAIRISRPGDTLRVM
                     KGIYKEGTITVDKPIYLQGIDWPLLDGENKFEIMRIISDNVTVMGFRFRDVGVSYISD
                     NAAISLEAVKNCRIANNKFENSFFGIYAKNSSECDIVNNELIGNALVEMSSGNAIHLW
                     YSKKMIVKDNVVKKHRDGIYLEFVDESIVEGNVSQNNIRYGLHFMFSNHDKYLGNTFS
                     NNGAGVAVMFSRNIEMTNNTFELNWGTASYGLLLKEIYDGKIIKNKFLYNTIGIYGEG
                     ANRIEIMENEFMGNGWALKILGSCADNTISKNNFISNTFDLSTNNSRNYNNYDGNYWS
                     DYSGYDLNKDGRGDIPYRPMKLFSYLVSRVEPSIVLLRSFFIDIIDFAEKVTPMFTPP
                     TLVDSEPLMKPWKNVADN"
     gene            25549..26265
                     /locus_tag="VGA21_00765"
     CDS             25549..26265
                     /locus_tag="VGA21_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009580734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9649062.1"
                     /translation="MLQIINLHKSFGKNQVIKGVDLSIEQGKLTAILGPNGSGKTTII
                     KSILGMVIPDKGEIIFDGENIKKHWNYRKKLNYLPQIARFPENLRVKELLALVKVLRR
                     QESDEQEIIDRFALGNDLDKPLRNLSGGTRQKVNLLLTFMFNNPMIILDEPSNGLDPV
                     ALLALKELISSEMKKGKTILFTTHIMSLVEDLAEEIIFILEGKVYFQGTQPKLMELTS
                     ERNLERSIAAILSSNGVNKP"
     gene            26283..27065
                     /locus_tag="VGA21_00770"
     CDS             26283..27065
                     /locus_tag="VGA21_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009580733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX9649063.1"
                     /translation="MVKIFKYSFSDLIRSRWSLAYFGFYLITAVALLYLSTDLSRAVA
                     SLMNVVIILSPLIGMLFGVMYYYNSREFIELLLAQPIKRSSIFLGQYFGLAVSLSLSF
                     FLGILLPFIFIGSGEIKIWLNFITLIVSGVFLTFIFSALAYFVSIRHENKIKGFATAI
                     ALWLFMAVIYDGIFLLLLVFFNEYPTDKLALGIVMLNPVDQARILMMLKLDISAIMGY
                     TGAVFQKFFGTSLGMTMSLLMSALWVIFPVWGMIRYANRKDF"
     gene            complement(27111..29879)
                     /locus_tag="VGA21_00775"
     CDS             complement(27111..29879)
                     /locus_tag="VGA21_00775"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEX9649064.1"
                     /translation="MKHFILLFILFIGQFAIALAQELTTHPRLFVTGNDLITLQSWAV
                     MSNPMYSEGLKQLADEAVTLMDTGVLPEQDPGNSAYSAYYTEGFAHVLAFMSLVESDA
                     SRKADYKARAKSLLMYIIDRADNGIIPSGEEANYTTDELRYRRHNFSSSDRARWIGLA
                     FPLTVDWIYDVFSPAEKARIRRVFIRWIEMNMVAYPHANYFRNGPDLFPEIANYNNEA
                     WLELNDPQKRALRFAMNNYFMMLIRNSVMMAMSFDAGDDIADPALPNDYDGRLREYRE
                     KILHTWYFMTDYAFRHENNGGLSAEGIEYMPSMGYPLHLLLSLYTAGYRPEDTGSSGT
                     PWGAKVSINSNSHWESTVTGVLNSLSPNSVELPSRSGTYYQMAWYGDGEHFFAPEFMR
                     IFGPLGVYARIEGNTGLLNAIRWIETYAAPGGQARLFSRVRSKEEMNSSILYFMIFDP
                     ESAIEAQNNTLPDPHESFPTHYFAAGPGRILSRTDWSQSASWFNFNLGWTGIDHQHGD
                     GNMFDFFRKGEWLTKERTGYGNVAASSDYKNTLTIKSETDGLHLGTIGNIHARRGSQY
                     NLANSDGDPIIEAMTINDDFVFVTGNSTNLYNYDDGSGFRPILRNVSHAGRSIFWLKP
                     DLIIIYDRVETTNNGFKRFWLNLPDANPEIAGKEINALTPGGQNLKIMNMLPPASTIQ
                     IVTNDEDLDIEPYLSVYDPMQTQNDDYLQPTRLMIEANENPNSVRFLNLLIGQDAPQA
                     VTADLISWNGDFPMDGVRIDTLAFLFARNPNQNFVGFTHNRIAGVKRYYLSGFEPGSQ
                     FDIDMTETNLSLTPGVFATADNGGVLIINSDGTSPPVVAINYENTSMNIWPNPSDGRF
                     RIELPSQVPGEKRIQVLNLAGVEVFETEVQGLERNFELDLHHLNKGIYLITVSGRFAN
                     FFAKVVIE"
     gene            30012..30314
                     /locus_tag="VGA21_00780"
     CDS             30012..30314
                     /locus_tag="VGA21_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008624553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4286 family protein"
                     /protein_id="HEX9649065.1"
                     /translation="MVVYNVTVGIDKEVEQEWLNWMINHHIPRVLATGLFHENKIYKV
                     LTGDEENISYSIQYFAESLTMVEKYLNEYAPVLIQEHNEKFKNRHAAFRTLLQSVD"
     gene            complement(30311..30787)
                     /locus_tag="VGA21_00785"
     CDS             complement(30311..30787)
                     /locus_tag="VGA21_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014019695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX9649066.1"
                     /translation="MGIKIREGKKEDLPKTLELVKELAAYEKALDEVENTVEMMERDG
                     FGDNPVFGMYVAERVNQIIGVAIYYFRYSTWKGKKLYLEDMVITEEERGKGVGKLLFE
                     RVVKKAQEEQCTGMSWQVLDWNGPAIKFYEKYNATFDGQWLNCNFDRKQLANFKPD"
     gene            complement(30803..32944)
                     /locus_tag="VGA21_00790"
     CDS             complement(30803..32944)
                     /locus_tag="VGA21_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009580585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX9649067.1"
                     /translation="MKRFALFLFALIIGFSCSETEQKSANVNYPVTKKVDHLDTYFDQ
                     QIADPYRWLEDDNSEKTAEWVTNQNAATNSYLDQISYRETLKERLTELQDYEKLSAPN
                     HKGDYYYYYRNEGLQNQSVLYRKPTLDGEGKVFLDPNTFSEDGTISLAGTSFSKDDKY
                     FSYMISVSGSDWREVYVMDVETKEKLSDKIEWAKFTGTSWYKDGFFYQRFPQPGEGDE
                     LKSENIYGKIYYHKVGSPQLEDELIYEDQENPNNRFFAWVSDDERFLGIYTSASTSGN
                     ALKVKDLSMENSDFITLVDDFENDHSIIGNIGEILFIKTNLDAPNSRVVQTTLTNPAP
                     GSWVDVIPESESVLNASMAGGKIFGSYLVDAKTEVKQFNTDGSLDKDIELPDIGSAFG
                     FGGDADDTELFYTFTSFITPGTIYRYDIATGKSVEYQRPDVKFNPDDYESTQVFYNSK
                     DGTRIPMFIIHKKGIDLNGKNPTWLYSYGGFNISLRPSFSTYRILWLENGGIYAQPNI
                     RGGGEYGEDWHEAGTKMQKQNVFDDFIAAGEYLIAEGYTSKEFLALQGGSNGGLLIGA
                     TINQRPDLAQVAFPQVGVMDMLRYQNFTIGRAWAADYGTSEDSEEMFEYLKGYSPLHN
                     IKEDVTYPAVLVTTADHDDRVVPAHSFKYAATLQEKAGAGPNPLLIRVETKAGHGGGT
                     PTSKAIELYADLYSFAFYNMGIIPDVAKTKL"
     gene            complement(33017..34291)
                     /gene="serS"
                     /locus_tag="VGA21_00795"
     CDS             complement(33017..34291)
                     /gene="serS"
                     /locus_tag="VGA21_00795"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020596305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEX9649068.1"
                     /translation="MLFVPYLRENIENAIHGLEKRNFPNAKKALATVLELDANRKKTQ
                     ASLDSHLAESNALSREIGALMKAGKKEEAENKKRKSVTLKEAAKREEEELNQLEDRLK
                     DLLYTIPNIPNKMVPAGKSAEDNEVVTLWGEKPNLVDDPLPHWELIKKYDIIDFELGN
                     KISGAGFPVYKGKGARLQRALINFFLDEALKSGYTEIQPPIVVNEDSGYGTGQLPDKD
                     GQMYKLIDENLYLIPTAEVPITNLYRDVILKEDELPIQNVGYTPCFRREAGSWGAHVR
                     GLNRLHQFDKVEIVQIQHPDNSYKALDEMCEYVMGLLKKLNLHFRQLRLCAGDLGFTS
                     SITYDMEVFSGGQKKWLEVSSVSNFETFQSNRLKLRFKDSTGKTRLVHTLNGSALALP
                     RIMAAIIENNQSSVGIAIPEVLVDYTGFDKID"
     gene            34572..36233
                     /gene="rho"
                     /locus_tag="VGA21_00800"
     CDS             34572..36233
                     /gene="rho"
                     /locus_tag="VGA21_00800"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013454911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEX9649069.1"
                     /translation="MYSIDDLNVKLLSELKEIAEELGFKNHKKMSKTDLVYKILDQQA
                     LKPDDAEQPLKASSKEKEKEKDKAEDLFDSFEMEFDESITSFDSPSEDVDKGKANSGS
                     SDGEPEAKEKETEPASTNANLESSAKAVNGKEDIGDRKPRGDLDSRDNRDSNENRDRR
                     ENRDSRDQNRGKKSGAAALIKEFDGVIENDGVLEIMQDGYGFLRSSDYNYLASPDDIY
                     VSPSQIKLFGLKTGDTIKGQIRPPKEGEKYFALLRVEYINGKTAEEIRDRVSFEYLTP
                     LFPNERLNLSTKPDRFSTRILDLFAPIGKGQRGMIVAQPKTGKTVLLKEIANAIAENH
                     PEVYLMILLIDERPEEVTDMARSVKAEVISSTFDEQAERHVKVASIVLEKAKRMVEVG
                     HDVVILLDSITRLARAYNTVVPSSGKILSGGVDANALHKPKRFFGAARNVENGGSLTI
                     IATALIETGSKMDEVIFEEFKGTGNMELQLDRKLSNKRVYPAIDVPASGTRREELLMD
                     KEELQRVWILRKFMSDMNSNEAMEFLLDKMKGTRNNEEFLVSMNG"
     gene            complement(36236..37444)
                     /locus_tag="VGA21_00805"
     CDS             complement(36236..37444)
                     /locus_tag="VGA21_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002692992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9649070.1"
                     /translation="MKIGLLREGKIPMDHRVPLIPSHASELQSKYPNVRVIAQSSDIR
                     CFTDDEYRGAGIEVRNSIEDCDVLLGVKEVPVEKLIAGKTYFFFSHTIKKQAYNKKLL
                     QEILKKEIMLIDYEVLTDSERNRIVAFGRYAGIVGAYNGILAFGLRYKLFKLRRAHEC
                     FDLDDLKTEYTKVKLPNIKILITGGGRVAKGAIEVLLGMGIRKVNPSDFINERFKEPV
                     FSQINPREYNKHKEGKEFKKKEFHQYPERFGSDFLKYRKACDLLIAAAYWDPSAPKLF
                     TREDMLKVDFNIRVIADITCDIDGSIPSTKKATTIDEPFYDYNPEENRIELPFVDEAN
                     VSVMSIDNLPAELPRNASEDFGRDIIDKVLPSLIEEDKDKIIERATITSAGNLTPPYQ
                     YLQDFVAEQA"
     gene            37507..38106
                     /locus_tag="VGA21_00810"
     CDS             37507..38106
                     /locus_tag="VGA21_00810"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF018949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HEX9649071.1"
                     /translation="MKIDITYMWLIGAWAIYFSLHSLMARQNVKQWAAINLSLNKYYR
                     LVYSMISTIGLFALLVFNGLYDQTLIISRTKVIMYLSLMLATSGVLIISRSFKVYSLK
                     KFLGLSKKEESELQIKGILKYVRHPIYCGTILIIIGYWFFNPTIATTVTCITTFLYLP
                     IGILLEEKSLIREFGETYLNYKRKVPALIPKLWSSSSAN"
     gene            complement(38131..38322)
                     /locus_tag="VGA21_00815"
     CDS             complement(38131..38322)
                     /locus_tag="VGA21_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015265132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649072.1"
                     /translation="MAKSKKKPRVNPQLEGFELKVDSFGELKSTMDIDKINEFLNRNV
                     DDKKLTKKKMKSKKKGRSK"
     gene            complement(38327..39847)
                     /gene="gltX"
                     /locus_tag="VGA21_00820"
     CDS             complement(38327..39847)
                     /gene="gltX"
                     /locus_tag="VGA21_00820"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015813707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEX9649073.1"
                     /translation="MSVRVRFAPSPTGPLHIGGVRTALYNFLFAKKNKGKFILRIEDT
                     DQNRFVDGALEYIMNSLEWLGIRPDEGIREGGDFGPYRQSQRKNIYQKFADQLIESGN
                     AYYAFDTAQELEAMRARLKEAKSDTQLYNYLTRSEMKNSLSLDKEETRNLMASESERV
                     IRLKVPENTSISFTDLIRGNVEFNSSNIDDKVLLKSDGMPTYHLANVVDDHLMKISHV
                     IRGEEWLSSTPIHVLLYQGFDWKSTMPKFAHLPLLLKPDGNGKLSKRDADKQGFPIFP
                     ISWKDSNTGEVSEGFREKGFLPEALINFLAFLGWNPGTEKEIYALAELCEDFSVERIQ
                     KGGSKFDFEKAIWFNQQYVHKLPKEKMISSLKISLEENQIKYSDEKVSEAVELMKDRV
                     SFLHELFTEARYLWEAPSHYEENVVARKWNADTKKAIQSLMNTWHSLDLFTASNIKES
                     LESTLVTIEMPAGKVMQALRHSITGKGGGPDLIGIIAFLGKDESLKRMRTTFNSIN"
     gene            complement(39886..40347)
                     /locus_tag="VGA21_00825"
     CDS             complement(39886..40347)
                     /locus_tag="VGA21_00825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649074.1"
                     /translation="MRILPYYHETLVLPFTSDKVIQILSENTLHPVERKSGHQKKFIG
                     HIESDSFRISEKLDRPDNFIPFIKGSIEKTSKGSIIFTSYNLLFSTALFVSFWSVVTM
                     LLCLLFLFQYNNNLYAVISMAAFLFNYYFTSTHFHRKVKGSRKAFYSLFTE"
     gene            complement(40344..40724)
                     /locus_tag="VGA21_00830"
     CDS             complement(40344..40724)
                     /locus_tag="VGA21_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013454356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX9649075.1"
                     /translation="MMKNVVESKKAPAPIGPYSQAIWANDILFVSGQVAIDRENGAMI
                     TNEIKEETHQVMKNLGNILEEAGLNFENVVKCSIFVKDMGNFQAINEAYGSYFGAIPP
                     ARETVEVSALPKNVNVEISCIAIK"
     gene            40797..41456
                     /locus_tag="VGA21_00835"
     CDS             40797..41456
                     /locus_tag="VGA21_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013454357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HEX9649076.1"
                     /translation="MKILVVGNIDQFEELKLKFGADHVYSSLEDSDNPEGYDPEVVFD
                     FVAEEIPESLENYEKIECPVFFSIVKTSLAHQNIYYPVKDQWFGFNGLPTFINRELLE
                     VSIFNSKGEGYLSKIAHGLKTDYVLVEDRVGMVSPRVVCMIINEAFYTNAEGTASEKD
                     IDLAMKLGTNYPLGPFEWAEKIGIENVYEVLEAVYEDTGDERYKINPQLKKKYLKEIT
                     R"
     gene            complement(41472..43307)
                     /gene="glmS"
                     /locus_tag="VGA21_00840"
     CDS             complement(41472..43307)
                     /gene="glmS"
                     /locus_tag="VGA21_00840"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010611557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEX9649077.1"
                     /translation="MCGIVAYSGYREVYPLLIKGLKRLEYRGYDSAGVAVMNSDLKVF
                     KKKGRVIDLENILKKEDLESHLGMGHTRWATHGEPNDTNAHPHYSSNRKLAIIHNGII
                     ENYTAIKQELQRKGHVFQSETDTEVLIHFIEDIYENEKCSLEEAVRLALNKIVGAYAI
                     VVMSSDHPEMLVAARKGSPLVIGVGKGELFLASDATPIIEYTKDVIYLNDQEIVVFEG
                     AEFRIKNTQDIQMTPYIEKLDLELESIEKGGFEHFMLKEIFEQPKSVLDCMRGRLNSE
                     KGYLMLGGIREYANKLENAERIVIVACGTSWHAGLVAEYFFEELCRIPVEVEYASEFR
                     YRNPVIKEGDVVFAISQSGETADTIAAIEMAKIKGAIIFGVCNVVGSSISRITHEGAY
                     THAGPEIGVASTKAFTAQLIVLFMIGLRIAHQKGTIKERDFRNMLVEMENLPSKLERA
                     LAVNERIRTIAETFSESRNFLYLGRGFNFPVALEGALKLKEISYIHAEGYPAAEMKHG
                     PIALIDKEMPVVFIATQDSSYEKIVSNIQEVKARKGRIIAIVTEGDTIVRDMADFVIE
                     IPKTHEALTPILSVIPLQLLAYHIAVLRGCNVDQPRNLAKSVTVE"
     gene            complement(43310..44677)
                     /locus_tag="VGA21_00845"
     CDS             complement(43310..44677)
                     /locus_tag="VGA21_00845"
                     /inference="COORDINATES: protein motif:HMM:NF025458.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4270 family protein"
                     /protein_id="HEX9649078.1"
                     /translation="MSLLGRKVLLPALVAAFFLSCEDPGEIGITESLPNSFEVTFVDL
                     PLNPKVAFMDSVNSTNSSSLLFGRYQDANFGVISATAYMELAITEIDFEIPENSIFDS
                     LVLFMDVNYAYGPQPNAVMSLEARTFQGNIETSVYFTKDSIETPLVLGPSSGLLGARN
                     RSYNSGDLNTFYFKLNDGYGEQLLNNMIYDQDSTFRNQAYFREYLSGLKVYSTNINGG
                     VLGFNPVADSTRMSLFYHYFDDDGEKVDEEYQFRVRRGFSNVRSDFTGTPFQNLGNSY
                     SDFDPQNEFAYHNPALGLFLKLDLTSLYNFKDTAANVIINRADLIINVDKTVTSLNPP
                     DGIQYYQVNAGNRFVISDLDSIPRSIQIESPNILFLPTLANDPFLFGYDSINYMYGEQ
                     VTFFVNNILSEKLSDSSLVVLPLVPARDGTRPERIYYPRVNSVDQFVVRKNDVKLRLY
                     YSKFD"
     gene            complement(44661..45473)
                     /locus_tag="VGA21_00850"
     CDS             complement(44661..45473)
                     /locus_tag="VGA21_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018620002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HEX9649079.1"
                     /translation="MAKFRILYVASEINPFLQTTEVADFVRKLPQAMQERGMEIRILV
                     PRFGLINERKNRLHEVVRLSGINISVGDEEKPLIIKVASIPNAKLQVYFIDNEDYFQR
                     KSVFHDKENKFYDDNDERAIFFCKGVIETVRKLGWGPDIVHCTDWMTSLIPLYLKTTY
                     KNDPLFKETKTVFTVYNNKFSHKFKSNLIEKVKMIDIEDQMLSNLKTGDFEGFVKLGM
                     EYSDAVITSDQSVNKSLNGLFEKMARAKKVDTFEKGDSFTDSYFNLYNELVG"
     gene            45588..46460
                     /gene="panC"
                     /locus_tag="VGA21_00855"
     CDS             45588..46460
                     /gene="panC"
                     /locus_tag="VGA21_00855"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012928946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEX9649080.1"
                     /translation="MIYMKVFSTIKSLQNHLKSVRAQKRSIGLVPTMGSLHKGHHALI
                     AQSRKENDVTVCSIFVNRKQFNNKKDLQKYPRNLANDLQFLKSWGCDTVFAPSEKEIY
                     PNSEAELTINFGTVDKILEGKFRPGHFSGVGIIVSKLFNIVTPDRAYFGLKDIQQCFI
                     IRHLVDELSFGVKLRFLKTVREENGLAVSSRNKRLSKKEFDVASDIFRILNLAKKEIM
                     VTGDIKGGISNSKKILKGMKPIELEYMEPVNMKDFSIVTSEIQKDSRVAICVAAYVGE
                     VRLIDNVIFKLTHE"
     gene            46453..47487
                     /locus_tag="VGA21_00860"
     CDS             46453..47487
                     /locus_tag="VGA21_00860"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEX9649081.1"
                     /translation="MNKTQINSWIQYLIIIVITVVLVWFAVSNIETGKEESKLDFLLE
                     VWNGVDKGLLVLSGVVALLSHVVRAERWKLSFQPMGLHISLKHSFLSVMVGYFINLLI
                     PRGGEISRCVNLNRLDSIPVDRSFGTVLAERAVDIVFLVFFVLLGFLLEFSKINLFLS
                     SLQYDKSKDWSLIIKYVLLLVFALIAGIIFLRLLLKSGKPRVLRTYIKFKTIMKGIKE
                     GLMVVIRLKQKSLFLFYTVIIWLLYLFMSVFVLMAFEETQHLGFVAALAIFSIGGIAM
                     SLPLPGGTGSYHILVPAGLVALYSIPLDKATAFTIIFHGWQTIIIIIFGAGSLFYSYY
                     LHNKKRRWNP"
     gene            47564..48259
                     /locus_tag="VGA21_00865"
     CDS             47564..48259
                     /locus_tag="VGA21_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013454174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HEX9649082.1"
                     /translation="MGLIVIMIFFGILSFVVSSRLKSKFRKYSETPIRSNLSGKEIAE
                     KMLADHGIYDVKVISTPGHLSDHYNPATKTVNLSEAVYNGKNAAAASVASHECGHAVQ
                     HARAYTMLEFRSALVPMQNISAKVINVIFMISLFGGFFLFNAFPTNLVLTVIIGAYTI
                     MTLFSLVTLPVEFDASKRALAWVTDKGIVSSSEHEMAKDALKWAALTYVVAAVGSLVT
                     LLYWVFVLLGNRD"
     gene            48293..49432
                     /locus_tag="VGA21_00870"
     CDS             48293..49432
                     /locus_tag="VGA21_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018476437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEX9649083.1"
                     /translation="MNFQFTEEQLAVKEAAREFAQSELLPGVIERDENQQFPKEQVKK
                     LGELGFLSMMVDPKYGGGGMDTVSYVLAMEEISKVDASCSVIMSVNNSLVCWGIEKFG
                     SKEQKEKYLTKLASGAVIGAFCLSEPEAGSDATKQNTTAIDMGDHYLLNGTKNWITNG
                     NSAGIYLVIAQTNREKRHRGINAFILEKGMEGFIVGKKENKLGIRSSDTHSLMFTDVR
                     IPRENRIGEDGFGFNFAMKTLNGGRIGIASQALGIASGAYELALKYSHERKAFGKEIF
                     NHQGIKFKLADMATKIEAARLLCLQAAVLKDQGKDFVKEAAMAKLYGSQTAMEVTVEA
                     VQIHGGYGYVKEFHVERLMRDAKITQIYEGTSEIQKIVISRELLK"
     gene            49573..50493
                     /locus_tag="VGA21_00875"
     CDS             49573..50493
                     /locus_tag="VGA21_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020606848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX9649084.1"
                     /translation="MEDYNKIIESLGVRFNKAGNINILQPATVTNYYDVENMILVLNR
                     GEIRFGEDNKGVLEGEVLFIPGGRQIPITYGTGKAHVVNNEEFTAKRKNYFRSYINIE
                     SDDQKYDDFTYVSFDAKVFDSVSFFTSLDVPPFVINDPNINALINSILKEKGSDLEGK
                     DRVIKLQTEYLVIEIVRYIIKNRLFVEQLATNSTYFKDPRLIDIFGYIKENLGGDLSN
                     KTLASVANVSEDYVGQYFKMLTGINPQDYIEYQRMERAVDLLRTTKKSIREIGKEVGY
                     KDTAYFCRRFKMMFGIPAGKMRRRESLMNL"
     gene            complement(50479..51237)
                     /locus_tag="VGA21_00880"
     CDS             complement(50479..51237)
                     /locus_tag="VGA21_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007655840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047294 - phosphoglycerol
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0006650 - glycerophospholipid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranylglyceryl/heptaprenylglyceryl
                     phosphate synthase"
                     /protein_id="HEX9649085.1"
                     /translation="MIISQIIDRKQKGIKGFAVLIDPDKVEEVKDLQGLINIAIENNV
                     DYFFVGGSFISRNNIGLVVRKLKDNSHIPVILFPGNYNHIDSHADAILFLSLISGRNA
                     EYLIGQHVIAAPFLRKTSLEILPTGYMLVNDHAKSSAAYVSNTIPIPNDKTEIACATA
                     MAGEMLGMQMIYMDAGSGSECPIPPDMISKVSKSINIPLIIGGGIRTEIDAMHALKAG
                     ADIIVVGNGIENSKDLLINISLTVSEYNESLKVH"
     gene            complement(51215..51577)
                     /locus_tag="VGA21_00885"
     CDS             complement(51215..51577)
                     /locus_tag="VGA21_00885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649086.1"
                     /translation="MKNPKIEKAALLENSERYKENFAEDLSEMTSKIERGIITGLIVG
                     GLAYVTYLFVKGLTSDSDQTDNQKPEDHQKPGNAFGHVAKYVAEAATLLLINLAKEKL
                     IEYLSERKQVEDDNQPDN"
     gene            complement(51558..51947)
                     /locus_tag="VGA21_00890"
     CDS             complement(51558..51947)
                     /locus_tag="VGA21_00890"
                     /inference="COORDINATES: protein motif:HMM:NF018980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEX9649087.1"
                     /translation="MEEKKGIFSFLKLDRIVNNLIGFVENRIELLKLELREEAAETGA
                     KLLILACFAILILFFTIFLSFFLSDYLNDILESRYWGFGIVSGFYLFLILLLALLQKL
                     LNLKIRIRKYIESYLSTTKDESEESEN"
     gene            complement(52021..52710)
                     /gene="phoU"
                     /locus_tag="VGA21_00895"
     CDS             complement(52021..52710)
                     /gene="phoU"
                     /locus_tag="VGA21_00895"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEX9649088.1"
                     /translation="MMTHLEKELSKLKQSILEMMDLAKFQLKKSEEAFINSDFKLAKE
                     ITRNEKRMNAMELSIDLDCENILALFQPVATDLRFVISMLKINSDLERIGDYADGIAD
                     YVLEMDEAISSDAINATEVGKMFHITLSMLTDISTALNDEDTSLARKVYKKDAELNSL
                     NSMASQTIRKFIKKDPDATRSLLFLFSVIRKLERVGDHIKNIAENAVFYIEAEIMKHK
                     KLQKDPNKKEK"
     gene            complement(52722..53522)
                     /gene="pstB"
                     /locus_tag="VGA21_00900"
     CDS             complement(52722..53522)
                     /gene="pstB"
                     /locus_tag="VGA21_00900"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017733771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HEX9649089.1"
                     /translation="MELKEEGYKKNDHESRSFKLEASHVDVWYGQFHALKNVSMGMTA
                     NHVTALIGPSGCGKSTFLRLFNRMNDYIDEFRMEGEIRLDGFKIYGKKIEVEQLRKDV
                     GMVFQKPNPFPKSIFENVAYGLKIQGITNKKFISEKVETALKQSDLWGEVKDNLKKSA
                     LALSGGQQQRVCIARTLAVEPSVILMDEPTSSLDPISTARIEELIFQLKEKYTIVIVT
                     HNMQQAGRVSDNTAFFYLGELVEHGKTKQIFTNPKHERTENYISGRFG"
     gene            complement(53548..54405)
                     /gene="pstA"
                     /locus_tag="VGA21_00905"
     CDS             complement(53548..54405)
                     /gene="pstA"
                     /locus_tag="VGA21_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007652353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEX9649090.1"
                     /translation="MNNRRKNRIADKAFQVWGIACTFIGLVLLVIFIGDILIDGLGRL
                     SWDFITALPSRRPESAGIWTAMMGTAWVLVFTTIISFPLGVAAGIYLEEYAKKSHLAN
                     LLEINISNLAGVPSIIYGLLGLEIFVRVMNLGPSVLAGSLTLSLLILPIVIVATRESI
                     KAVPGSIKEASFALGATKWQTIRNQVLPASFGGILTGVILALSRAIGETAPLIVIGAL
                     AYVPFAPKSPMDEFSVLPIQIFNWVSRPQQGFITNAAAAIIILLLITLVMNGIAVYFR
                     NRWQKKMKW"
     gene            complement(54398..55276)
                     /gene="pstC"
                     /locus_tag="VGA21_00910"
     CDS             complement(54398..55276)
                     /gene="pstC"
                     /locus_tag="VGA21_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017733769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEX9649091.1"
                     /translation="MRKIKEYLIEKSLLLSAFITILTTIGIILVLTVEAVNFFSEVSL
                     ISFFTDTEWTPLFEQKHFGILPLLTGTLLTTFIAIFVALPIGLSIAIYLNEYAPKGFR
                     KTIKPALELLAAIPTVVYGFFALMVVTPFLQQIYPDLAGFNALSAGIVMGIMIIPFIS
                     SLSEDALFAVPKSLREASYGMGSTRLQTAMLVTVPAASSGIVVSIILAISRAVGETMI
                     VAIAAGQQPRLTFDPTVPIETITAYIVQVSLGDVQHGTLEYRTIFAAGITLFALTFLL
                     NNISFFLRKKYRQKYE"
     gene            complement(55346..56299)
                     /locus_tag="VGA21_00915"
     CDS             complement(55346..56299)
                     /locus_tag="VGA21_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009581628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX9649092.1"
                     /translation="MKTGITIHTILTSALLLYACGSNSGESAKADATELSGSISIDGS
                     STVYPITEAVAEEFRAISPRVRVTIGVSGTGGGFQKFARGETDISDASRPIKEKEIAA
                     CEKNGISYVELSVAYDGLTVVVNPENNWVDNFTVEELKKIWEPAAQGTIMKWNQIRPD
                     WPDEEVHLFGPGVASGTYDYFTEAVVGVTGSSRGDFTASEDDNVLVQGIAGDKFGLGF
                     FGLAYYEENKDKLKVVAVDGGHGPILPSLETVRSGSYSPLSRPLFIYVNSTAIERMEV
                     ISFVDFYLENASLLAAEVGYIPMPDDLYSAEISKFNDFTKR"
     gene            complement(56409..57611)
                     /locus_tag="VGA21_00920"
     CDS             complement(56409..57611)
                     /locus_tag="VGA21_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014770907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEX9649093.1"
                     /translation="MMNKLMLIVMIMAISNFNAYSQTKEQNTVETEWYDNISIRGYAQ
                     VRYNRLFETNENLGCEQCDKSWGKGGGFFIRRMRIIFYGQITRNVYFYVQPDFASSPG
                     SDKLNFVQLRDAYFDIGLDKENEFRFRIGQSKVPFGFENLQSSQNRLPLDRNDGLNSA
                     VSNERDLGVFFFWAPAGKGKLLSSLVRTGVKGSGDYGILGIGIYNGQTANSLEINKEP
                     HFVARATYPFEVRSQVFELSVQGYTGNYVIPQSNLSAGVKYKTDLSYPDQRIAGTFIL
                     YPKPFGIQAEYNIGEGPEFNNATDSVEVKSLRGGYLTASYLTKIKNQIIIPFTRVQYY
                     NGGKKHERDARSYEVSEFELGIEWQPLKQFELVLMYTHSRRRYEDFINQDNLQEGSLL
                     RIQAQLNF"
     gene            57873..59555
                     /locus_tag="VGA21_00925"
     CDS             57873..59555
                     /locus_tag="VGA21_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009581627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HEX9649094.1"
                     /translation="MKFGFLDLLELIGALGLFIFGMKIMSEGIQKAAGSQMRRILRAM
                     TSNRFKGIMTGFAITSIVQSSSATTVMVVSFVNAGLLSLMEAIGVIMGANIGTTLTAW
                     LISIIGFKMKIVSISLPMIAFGFPMLFASKSTTKAWGEVLIGFAILLIGLDELKNTVP
                     DLSANPQMFEFLSRYTDLGVLSTLLFILIGTLFTIAVQSSSAAMALTLVMANNGWIPF
                     EMAAAMVLGENIGTTITANLAAIVGNVYAKRAARAHTIFNLIGVIWMIILINPFLRTI
                     GNYLISAQSMNPFMDATAIPIALSVFHTSFNILNTLLLVWFTPLIAKAVEKIVQSKGD
                     DEFHLEFIGKGIIRTPDISILEARKEIAKFGEITTKMSGFLQALLFKNKEKKRMKWMD
                     KIQKYEEITDRVEVEVANYLTKVAEGNISEDISVKVRSLLSINNDLERIGDLFYQMSL
                     AVQRQNDSIHDLDKNQLDGLQEMFRLIDKAFEVMNKNLNSNYSEVNINEAKDIEKRIN
                     AQRDDLKRTHLKHIEKGHYNVMAGMVYSDVFQTLERIGDHIFNVSEAVIGEI"
     gene            complement(59558..60067)
                     /locus_tag="VGA21_00930"
     CDS             complement(59558..60067)
                     /locus_tag="VGA21_00930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649095.1"
                     /translation="MINVLVKRIGYFILLALIGSCASKVGSQTQSSGYSEDISLYRPM
                     YPDPQTDTGSYPLAEVEQLTFVEPTASIAADLDSLLDSIASYTRSRGYYQVYTIQIYN
                     GTSSTAANDAKALAYREFSDLIPVLEYQQPIFKVKVGLFYERLDAQKTLEKMRKVFQN
                     AILIPERVY"
     gene            complement(60060..63413)
                     /gene="secA"
                     /locus_tag="VGA21_00935"
     CDS             complement(60060..63413)
                     /gene="secA"
                     /locus_tag="VGA21_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009194402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEX9649096.1"
                     /translation="MIRLLTKGIAAVFGSKSQRDIKSVMPLVEQTLREFDTLVNLNDG
                     QLRDKTSEVRDRIRQHLAEIDDQLKSLHDQIDQNPDLNIHEKEDIFAQIDSLEEKRNK
                     NLEDVLLEVLPLAFAIMKETARRFKENHKMEVPATMHDKQMAAKFQHVEIKGESAIWH
                     NKWLAGGNEITWDMVHYDVQLIGGIVLHQGKIAEMATGEGKTLVATLPSFLNALADRG
                     VHIVTVNDYLAKRDSEWMGPLFQFHGLNVDCIDKHQPNSEDRRNAYRADITYGTNNEF
                     GFDYLRDNMARDPRELVQRKHHYAMVDEVDSVLVDEARTPLIISGPIPRGDEHEFHDL
                     KPRIAKLVEAQKKLVSQYLIDARKLIAENSKDGGLSLFRAFRGLPKYKPLIKYLSETG
                     NKQVLQKTENFYLQDNSKMMPEADSPLYFTIDEKHNQIELTEKGIDLITGDDEDKSFF
                     ILPDIGAEIASLENDASLSDKERLARKDELIKDYSIKSKRIHSVNQLLRAYSLYEKDT
                     EYIIDGGKVKIVDEQTGRVLEGRRYSDGLHQAIEAKENVKVEDATQTYATITLQNYFR
                     MYHKLAGMTGTAETEAGEFWQIYKLDVVVIPTNRNIVRKDMEDKVYKTIREKFNAVVD
                     EIVDLTTNGRPVLVGTTSVEISELVSRMLNLRKIKHNVLNAKQHQKEAEIVAQAGLPS
                     TVTIATNMAGRGTDIKLTPESRAAGGLAIVGTERHESRRVDRQLRGRSGRQGDPGSSQ
                     FFVSLEDNLMRLFMPDRIARIMDRLGLQEGEVIQHSMITKSIERAQRKVEENNFGIRK
                     RLLEYDDVMNSQRELIYKRRRNALYGERLELDIMNQFYDASENIVLNAKGANDYDSLK
                     LNSLSVFSIDFKLSQEEFTSTNDSDLSEKLYQQAYDTYQRKNLTIAEKAYPIIKDIHK
                     TRGATIENILVPFTDGTKQIGVSANLVKCVDTKNKELIKSMEKNICLAIIDQQWKDHL
                     REMDDLKQSVQNAVYEQKDPLLIYKFEGFDLFKQFVARVNEETITFLAKADLPVQQPD
                     EVQEARQQRSQPRLSERKDESRSLLSGSGGGQSRQPIQKPAPMKSEKMAGRNDKVSVQ
                     YTDGTIKRDVKYKKVEDDILNNKCILIDD"
     gene            complement(63472..64251)
                     /gene="dapF"
                     /locus_tag="VGA21_00940"
     CDS             complement(63472..64251)
                     /gene="dapF"
                     /locus_tag="VGA21_00940"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020532806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEX9649097.1"
                     /translation="MELNFEKYQATGNDFVMIDNRDDFFDKENKGLISKLCNRRFGIG
                     SDGLILIEEDDKYDFRMEFFNPDSSKSLCGNGSRCAVRFAKSLGMVKNETEFSAFDGV
                     HEATVKGELVSLKMRDVSSVILMHDGFFMNTGSPHFVQFVAKINQYNVQREGKKIRHG
                     GLFGTSGTNVNFVEEKGDDTIFVRTYERGVENETLSCGTGVTAAALASSYKMLKSPVK
                     IKTRGGNLQVAFQQSGNNSFKDILLTGPAEKVFKGSISIKS"
     gene            complement(64263..64484)
                     /locus_tag="VGA21_00945"
     CDS             complement(64263..64484)
                     /locus_tag="VGA21_00945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649098.1"
                     /translation="MLKFLLILFAFMYLTFKLGGYIMRFLLGNSGKENQSFKQKPKGG
                     NVSINFDPKDKRNQKGYQGGEYVDYEEVD"
     gene            complement(64494..67307)
                     /gene="carB"
                     /locus_tag="VGA21_00950"
     CDS             complement(64494..67307)
                     /gene="carB"
                     /locus_tag="VGA21_00950"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010611410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEX9649099.1"
                     /translation="MPKDESIKSVLIIGSGPIIIGQACEFDYAGSQAARSLREEGIEV
                     TLINNNPATIMTDKVTANNIYLLPLTKKSIVEILKKHEIDAVLPTMGGQTALNLAIEC
                     QNAGIWDIFNVQLIGVDIAAIETTEDREKFRLKMNELGVNVCRGETATSFLEGKEIAQ
                     EIGFPLVIRPSYTLGGTGGGFVESPEEFEKALSHGLHSSPIHEVLIEQSIMGWKEFEL
                     ELLRDGAGNVIIICSIENFDPMGIHTGDSITVAPAMTLPDTVYQKMRNLAIKMMNGIG
                     KFSGGCNVQFAVNPENDDIIGIEINPRVSRSSALASKATGYPIAKIAAKLAIGYNLDE
                     LPNQITKTTSAFFEPAVDYVIVKIPRWNFDKFHGSDRRLGLQMKSVGEVMAIGRNFQE
                     ALQKACQSLEIRRNGLGADGKELTDQIAILKSLKNPSWNRLFHIYDAFKLGIPMKTIQ
                     ELTRIDKWFLYQIEELLILEKEIEKHSLDTIPEQLLRIAKEKGYADRQLGHLLRCLES
                     EVHKKRRDLGINRVYKLVDTCSAEFEALTPYYYSSFDSENESIPSKKKKVIVLGSGPN
                     RIGQGIEFDYSCVHGVLAAKECGYETIMINCNPETVSTDFDVSDKLYFEPVFWEHLYD
                     IIQHEKPEGVIVQLGGQTALKLAEKLERFGIKILGTSFENLDLAEDRGSFSTLLQKND
                     IPYPEFGVAEDAENAIELSKKIGFPLLVRPSYVLGGQSMKIVINEKELENHIVEILKN
                     IPGNKVLLDHFLDGAIEAEADAICDGVDVYIIGIMQHIEPAGIHSGDSYAVLPPYNLG
                     DFVIRQIEAHTKKIALALKTVGLINIQFAIKNDKVYVIEANPRASRTVPFICKAYDEP
                     YVNYATKIMLGLNKLSDFKFNPKKNGYSIKEPVFSFNKFPNVNKELGPEMKSTGESIY
                     FIDDLMDDYFLKIYSERNLYLSR"
     gene            complement(67417..67548)
                     /locus_tag="VGA21_00955"
     CDS             complement(67417..67548)
                     /locus_tag="VGA21_00955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649100.1"
                     /translation="MNNSVSQVAKGSQIIGRKKLKESQLEDRVQKPDGFVLNKPIEW"
     gene            complement(67598..68284)
                     /locus_tag="VGA21_00960"
     CDS             complement(67598..68284)
                     /locus_tag="VGA21_00960"
                     /inference="COORDINATES: protein motif:HMM:NF014045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEX9649101.1"
                     /translation="MKRRTAILVDGGFFVKRYRSINKVKNLNPERTAKDLWEMCLKHL
                     SQAKSETYDLYRIFYYDCLPYDKKQHNPLTGKSIDFSKTDQYRFQCAFFNELKKKRKI
                     ALRLGILEDRKRWIIKPSITKELLSKQITIEDLTEDDVQFDFNQKMVDIKIGLDIASI
                     TLKGQVDQIILISGDSDFVPAAKLARREGIDFLLDPMWNPIKPHLFEHIDGLVSKIKR
                     PKITMANSTQ"
     gene            68463..69455
                     /locus_tag="VGA21_00965"
     CDS             68463..69455
                     /locus_tag="VGA21_00965"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine dehydratase"
                     /protein_id="HEX9649102.1"
                     /translation="MNHPTLNKIEEALNSIGNYVRQTPVWEWKGSVKDNIFGSDTTII
                     LKLELFQHGGSFKTRGAMVNMLALTPEQLECGVTAVSAGNHAIAVAYAAKLLGSSAKV
                     VMPSTANPYRVTKCEELGAEVVLMENVHQAFAEAERIQKEEGRTFVHPFEGFNTVLGT
                     ATCGYEFITQAQNLDLAIIPIGGGGLIAGMAAAIKQVNPNCKVIGVEPEGADSMTRSL
                     KSGKPEGVEKVTTIADSLGAPYALPYSFSVVQKFVDEVMLISDQEMCEAMALLFTDAK
                     LAVEPAGAASTAALKKIVKSYQGKKIGLMVCGANIDLDNFSKYLKQGGLARNSK"
     gene            complement(69435..69908)
                     /locus_tag="VGA21_00970"
     CDS             complement(69435..69908)
                     /locus_tag="VGA21_00970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649103.1"
                     /translation="MKSAFLLFPLFIVCQSLAQSKVDSHDPVWSPDGNQIAFYSNRDG
                     DFEIYVVNSDGSNLRQITSNEFNDGSPTCSPDGKKLLFKSNRAGNSNFYLHELASGKT
                     HLLHKILRSWFKFHPILIFRCGNYWVPDLSNRFSPILFLNHKPKFDEGSLLTISC"
     gene            70073..70465
                     /locus_tag="VGA21_00975"
     CDS             70073..70465
                     /locus_tag="VGA21_00975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649104.1"
                     /translation="MANLRKVFILLMGLSVFTISCKRDAEPLPPFIGTWDLRKLVVKD
                     CVDPAENDEYLCPGGGCDIIVFSSQKVRLDGTAYDYTVMGEIIHVNLSPSIILVIQWK
                     IVISTLTLIIKDSVADGGCTFEYTYLKT"
     gene            complement(70783..71082)
                     /locus_tag="VGA21_00980"
     CDS             complement(70783..71082)
                     /locus_tag="VGA21_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HEX9649105.1"
                     /translation="MPYIICEPCVGTCDTACVDVCPVDCIHGPVNTEGSGAEVKEPGF
                     DPAGKQLYINPEVCIDCDACVPACPVDAIFEESEVPEEWKKYIPLNYEFFGQQAP"
     gene            71238..73097
                     /locus_tag="VGA21_00985"
     CDS             71238..73097
                     /locus_tag="VGA21_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009194951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HEX9649106.1"
                     /translation="MSETLAKEKQSQELEKVTIRFAGDSGDGMQLTGSQFTFTSAMLG
                     NDLATFPDFPAEIRAPQGTVEGVSGFQVQIGRIDIYTPGDLADVLVAMNPAALKSNLE
                     NVKKGGNIIVDQDAFTSKNWERVGYKANPVEDGSLADYNIVNAPISSMTKTALEGLDI
                     DNKSVLRSKNMFALGMMYWLFDRSLKETEKFLDAKFKKMPVLAEANKKALYAGFHYAE
                     TVEALPSSYKIPPAVIEKGKYRHISGNVATAWGLMAAAQKMKKKLFYGTYPITPASDI
                     LHELSRYKNMGVITLQAEDEIAAVCSSIGASYAGQLGATASSGPGIALKGEAIGLALM
                     AELPLVIIDVQRGGPSTGLPTKTEQADLNQAIYGRNSESQVIVLAASTPSNCFDFAFE
                     ASRLSVEHMTPVILLTDGYLANGSEPWKFPSMSELPDIKVPAINVKDNGQPFFPYSRD
                     PETLVRKWAVPGTPGLEHRIGGLEKQDQTGNVSYEAANHEMMVKLRADKVAKVANRIP
                     DQSVYGSGSGKLLVVGWGGTFGALYTSVKDAQEVGKDVDLAHFNYIFPLPKNTGDLLS
                     RYETILVCELNLGQFVGYLRSKFPGLNFKSYTKVQGQPFTISELKTQYDENLK"
     gene            73100..74122
                     /locus_tag="VGA21_00990"
     CDS             73100..74122
                     /locus_tag="VGA21_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009194950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HEX9649107.1"
                     /translation="MSNIDIKPLTKKDFASDQLVRWCPGCGDYSILSAMQLALPQLGL
                     NREDIVFVSGIGCAARFPYYMNTYGFHTIHGRGPAVATGVKLANPKLSVWEIQGDGDA
                     LAIGGNHFIHVLRRNVNINILLFNNEIYGLTKGQYSPTSRPGLVTKSTPFGVIDRPFH
                     AAELVIGAQGTFYARTIDTNPKLMSEIFVHAAGHTGTSMVEILQNCVIFNDKTHGAIT
                     SKENRDDYQLHLEHGKPMLFGKDNSKGIKLNGLKLEVVNLGENGTIESDILIHDAYDE
                     DPTLHLMLARMHPPLFPMALGVIREVRNTTYDELVESQYQNSVNNAKFSTMDELLNSG
                     ETWEVK"
     gene            74122..74472
                     /locus_tag="VGA21_00995"
     CDS             74122..74472
                     /locus_tag="VGA21_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009194949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEX9649108.1"
                     /translation="MEAQALVIFFLAGVVMVGAGVFVSYLVSPKSENSQKGETYECGV
                     PTIGQSMIQFKVGYYLFAILFLIFDVETVFIFPWAVVMKSVGMAAFIEILIFLVILGL
                     GLLYAWKKQALKWD"
     gene            74463..75029
                     /locus_tag="VGA21_01000"
     CDS             74463..75029
                     /locus_tag="VGA21_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009194948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HEX9649109.1"
                     /translation="MGLEGIGIDPKTREILKDFPGEIKKGPGSNIVITSVDGIVNWAR
                     SNSLWPLVFGTSCCAIEMMATGASRHDWSRFGTEVARATPRQADLIVIAGTIVNKMAP
                     VLKRLYDQMADPKYVIAMGACATSGGPFYYNTYSVVKGADHVIPVDVYVPGCPPRPEA
                     LLYGIMQLQKKIKTESLANRRDPMKDFI"
     gene            75038..75496
                     /locus_tag="VGA21_01005"
     CDS             75038..75496
                     /locus_tag="VGA21_01005"
                     /inference="COORDINATES: protein motif:HMM:NF012549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEX9649110.1"
                     /translation="MDNEKLKDFILKQIPAADFEENAQYLLAIVPASHIHSISLELKN
                     NPETRFDYLFCLTGVDYPDHMQVIYHLKSTELNHYTVIKARIDSREKPEIDTVCDIWH
                     TAEFHEREVYDLFGIVFRNHPDLRRIFLDEEWVGYPLRKDYKDPYNMIEY"
     gene            75496..76623
                     /locus_tag="VGA21_01010"
     CDS             75496..76623
                     /locus_tag="VGA21_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009194946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HEX9649111.1"
                     /translation="MKEIEEQEIQELESQEYFINMGPQHPSTHGVLRLVLSLDGEIIR
                     KVEPHLGYIHRSIEKMCEHDSYQQIIHLTDRMDYLSCHMLNEAVCLGVENGLEVEIPE
                     RVKVIRTIISELTRLSSHQLWWGVMGMDLGAITTFLYGFRDREMITDIFEETCGARLT
                     MSYNIPGGLMFDIHPNFQKRTKDFIVHFKKKLPEYDKLLSDNVIFRERTRGIGVLSKE
                     DAISYGVTGPSGRGSGFSCDVRKHNPYSAYPKVEFREILREEGDCFARYKCRIDEMWE
                     SLNIIDQLIDNIPEGDYKVQTRTVIKLPKGEFYQKVETARGEFGVYIISDGMKSPYRL
                     KFRSPGFSNLSALDKMSRGCKIADLVAIMSTLDLVIPDIDR"
     gene            76623..77708
                     /gene="nuoH"
                     /locus_tag="VGA21_01015"
     CDS             76623..77708
                     /gene="nuoH"
                     /locus_tag="VGA21_01015"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009194945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HEX9649112.1"
                     /translation="MKFLYDFGYLTGSIHEWLSARMDPPVVTLVETIIVAVCFMILFA
                     VLGLVLVYAERKISAFFQQRLGPMRVGKWGTAQTIADVIKLLFKEPLVNKDADKFLFN
                     LAPYIIMISAFLAIAALPFAKGLHALDFDIGIFYVSAVSSLGVIGILLAGWSSSNKYS
                     LIGAMRSGAQIISYELSVGLSLLTIIVLTGSLQLSTIVESQADGWWIFKGHLPAVIAF
                     LIFMIASTAETNRGPFDLAEAESELTAGFHTEYSGMKFAFFFLAEFANMFIVAAIGAT
                     VFLGGWMPFYIGGFTGFNAVMDFIPPIFWFLGKVAFMIFMMMWFRWTFPRLRIDQLLT
                     LEWKYLLPINLVNIVVMAFIGLMGWHF"
     gene            77720..78199
                     /locus_tag="VGA21_01020"
     CDS             77720..78199
                     /locus_tag="VGA21_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009194944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEX9649113.1"
                     /translation="MNGIIKYFKDIYYGLKSLVTGMAVTGHYITHPKEIITQQYPENK
                     DTLKMFDRFRGEVVLIHDENNEHRCTGCSACEVACPNGTIEIISKRVENEEGKKVKVI
                     DKFVYHLQMCTMCNLCIPACPTDAIKMSQDFEHAVFDRSKLTKILNKPGSKIMAGVK"
     gene            78207..78719
                     /locus_tag="VGA21_01025"
     CDS             78207..78719
                     /locus_tag="VGA21_01025"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEX9649114.1"
                     /translation="METYFFYFIAASMLVFSLLAVTSRRILRAAVYLLFSLAGTAAFY
                     FMIDYFFLAAVQLIVYAGGIVVLIIFSILLTSHIADKLERPDMMKNAFSAVLVLGGIA
                     LTAFVLGNFADGAPSATNQLNMQIIGEKLMNYKENGYVLPFEVISILLLAAMVSAIVV
                     AKRTKTKNDV"
     gene            78709..79029
                     /gene="nuoK"
                     /locus_tag="VGA21_01030"
     CDS             78709..79029
                     /gene="nuoK"
                     /locus_tag="VGA21_01030"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009194942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEX9649115.1"
                     /translation="MMFENIPLNHILILTISMFFIGVYGFLTRKNLVTVLISVELMLN
                     AVNINFVAFDKYLHPGTFEGHFFSLFVIAIAAAEAAVAIAIFFNLYRNIRSIDVESVD
                     KMKY"
     gene            79029..>79522
                     /locus_tag="VGA21_01035"
     CDS             79029..>79522
                     /locus_tag="VGA21_01035"
                     /inference="COORDINATES: protein motif:HMM:NF012868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEX9649116.1"
                     /translation="MNFEYTIWILLLPFLAFTINGLGSKKMGLASAWISITGIGIGAA
                     LSLIAAYQYFTKESEISSFYPYDVTWLKFTDSLSINMGILVDPISVMMLVVITVVSLM
                     VFIYSVGYMKGESGYARYFSFLSLFSFSMLGLVIATNLFQMYIFWELVGVSSYLLIGF
                     YYTKA"
BASE COUNT        23709 a        15809 c        16161 g        23843 t
ORIGIN      
        1 cttggcggag gtcagttggg tcgtatgatg atccaggccg gcattgattt caacctggat
       61 attcatgtgc ttgatccgga ccctgatgca ccttgtaaat ctttggcatc tacctttaca
      121 aaaggctcat ttaccgatga gctaaccgta tataattttg gtaaggacaa agacctcgtc
      181 acaattgaaa tagaaaatgt ttccgtatca gggttgaaaa gactggaact ggaaggtaag
      241 aaagtatttc cacagcctca aatcattgaa ttgattcaag acaaggtaaa acaaaaaata
      301 ttttacagaa ctcacaatat tcccaccgcg gaattcagaa ttgtgaagtc caaagcagaa
      361 cttgcaaagc attccgaaat aatcccggcg gtaaacaaac tggcaaaatc cgggtatgat
      421 ggaagaggag ttaaagtgat aagaactgca gaagacttga acgccggatt tgatgattac
      481 ggattattgg aacgatttgt aaatttcaaa aaggagattt ctgttatcgt agcgcgcaac
      541 gaaaaaaacc aaatcaaaac ctacccaccg gtagaacttg tttttcattc ggatgccaat
      601 ttggtagaat atcttttctc tcccgcggaa gtatcacggg aagtggaaat tctctccaag
      661 aaaattgcca ttgatctggc agaaaaactg aacctggttg gattactcgc ggttgaaatg
      721 tttgtaaccg aagacgattc aattttggtt aacgaaatag caccacgcac gcataatagt
      781 ggtcaccaat caatcgaggg aaatcaaacg tctcaatttg aacagcattt gagagccatc
      841 cttaaccttc ccttaggttc cactaaggtc attcaacctt cagctatggt taatttattg
      901 ggagaagaag gttattccgg attggcaatg tacgaaggac ttatggatgt cctatcagtg
      961 agcggtgtcc atattcatct ttatggaaaa aaaattacca aaccatttcg caaaatggga
     1021 cacgtaacca ttacagataa agatcttaat aaactcaggg agaaagctga atttgtaaaa
     1081 tcaaccttaa aggtaaaagc atgaaaaaac caatggtagg tataatcatg ggaagtgatt
     1141 ctgatctaac cataatgcag gaagcagccg cgattctcaa aaagctaagc gttccttatg
     1201 agatcaccat tgtttccgct caccgcacgc caaagcggat ggttgagtac gctgaaacag
     1261 ccaggaaacg cggccttaaa gtaataatcg cgggtgccgg cggagctgca caccttccgg
     1321 gaatggtagc gtctattact accttgccgg taattggcgt tcctgttaag tccggcaact
     1381 ccattgatgg ctgggactcc atcttatcaa tacttcaaat gcctggcgga gttcctgtag
     1441 ctacagttgc actcaatggt tctaagaatg ccggcattct cgccgctcag atcattggta
     1501 ccaattcgga aactgtgcgt aagagtcttg aaaagcttaa acatgagatg gctgtatctg
     1561 tggaacagaa agcggcccgg attgaaatga aaacggctgt caggaagaaa aaatagtatt
     1621 tcaaacattc atcagaaaca agaatcggaa cttagtaatt ccacccaatt tgttaaaaaa
     1681 tctccggcaa cttaaatcat tggattgtcg ggttgctgat ctgaaaccat ttctgaaggt
     1741 gatttgtcta cccgtttaag aactaatacc aagtcaggtc aggagataat ctaatttttt
     1801 cgtaatttag gataatttta cagtagcagg taaatgttaa atcccgggca aaaaggttgg
     1861 ctaaaggagt atcttgaatt ccgtaagagc atattggatt ctaagactga aaattccctg
     1921 cacaaaaaac ctgccccaag acatccggaa gaatcattat acggagcgtt acagccaaca
     1981 ggattaatgt atggccaccc gataaagttt tctcagcttg atgaaaagtt gatggaggaa
     2041 tggggttctc ataatgtaat gaaagttttg ctggcggaaa gtctgataaa cagttcattg
     2101 gtacaacacg aaactgaaat ccataatgaa gatgagttct acgaagtagt tctgaagacc
     2161 atctctaaca taaccggatt ttacaacaaa gtttaccccg aactttcaac ttcagagcga
     2221 acttttttgg gaaagaaaaa accacccctg gaagtagcag agaaaattct ggagaaaaga
     2281 attggattaa ctcacgagtc aagcaaaaat ttctgggtca atttcttcca taatagcttg
     2341 ttgtttttgg atatttactt ttttggacaa tggattcaca cgaattccga ggaggcggtc
     2401 accgaatttt tcagacatga gaaagaagat ttgcgttttt ctgttatccg ggtaattgtt
     2461 gccgccgccc acgcgaacca ggtaatagaa aaagaagagc agaaattatt tgaattcttt
     2521 cttgattcca gtgacttaac ttctgagaag aaaaaagagg cccacaaatt tcttgaggaa
     2581 ggcgttgatc tttcagaaat tgaggttccg ggcaattcct ggattctgag gaaatacttt
     2641 ctcgagctgg ccatactcac ggtttgggcg gataaaaaag ttgaggattc tgaagttgaa
     2701 ttcctggaaa acttaaccag gaagttgggg ctttcagaag aagatctcga aaatagcatg
     2761 atagccattg aaggcttcgt aattgaatat tgggatgagt tgggtcagtt acaaagtaag
     2821 cacgatctta cccaggtgag cgaaaaatac ctggagagaa tatccaaagt agtggcacat
     2881 aacaagaatc ggatagcaaa tgagattaat gaaagtaaac aactgaagat acttctggac
     2941 aaatatttgc gaggcgaaat ttctggcagc gatgctgaaa aacttcacat ggaattactg
     3001 gcaattgtga aaaccatccc aacgtttgtt atcatttcat tgccaacaac attcctcagc
     3061 ctgccaattt taattaagat acttccaaag agtgtttttc caggaaatga gaattcataa
     3121 ccgtgttacc agaccggtta tcttatattc aactgaatca agcataatca ttttttgacc
     3181 gcctgttatc tcatagcttg taagtacaag attattttct ccgtcgcgtc gcagttcaag
     3241 cttaagaata gtttctccac gaaagaggga atttacttcg gtaaccctgg tttcaatttt
     3301 cctcacatca tcccgatttt ccgcgtaaaa tacttttatg tactgaacac cctgaacctc
     3361 tttaaccgga ataaaatcga tttcctgaag attgctggtt aattcaccag gtccagccac
     3421 catttcatat acaccccgaa ggcttggtaa attaatgtcc gcatctctga aaagttttaa
     3481 ttcatccagc caaccaagac tatcgggttt caaaatcttt tcatcatgct tttcattcag
     3541 aaaagcagtt ttttttattt gataggttcc ggaacttagt atggaaacct gttcttctac
     3601 caatttttca aggctgaaat acagctcccc ggttttcact tcctgttccg cttgtgaaca
     3661 agaaaataaa caaagggaaa ctaacagaat ctttctcata aatcgattag gctttctcct
     3721 gtcattaagg aaggctttgg aatatccatt aattgcagaa tggtgggagc aacatcagcc
     3781 aatcgtccat ttttgatttt tccattaaag gatttatcca ctaaaataca tggaacagga
     3841 ttagtagtat gtgcggtatt gggagttcca tcttcgttga tcatgtaatc tgaattgccg
     3901 tggtcagcaa taataatcga actgtacccg ttttctaatc ccgtttccac aacatctttg
     3961 gtacacctgt caaccgtttc gcaagccttg atcgctgctt caaatactcc cgtatgtcca
     4021 accatgtctg cgtttgcaaa attaaggcaa acaaaatctg cccattgtgt ccttagctcg
     4081 gcaactatcc tgtcccgaac atcttctgca ctcatttcgg gtttgagatc ataagtggca
     4141 acttttggac ttgggcatag aattcttttt tctcccttaa attccttttc tcttcccccg
     4201 gaaaaaaaat aagtaacatg cggatacttt tcagtttctg caaccctgat ttgatgaagc
     4261 tccctttcag aaagtacctc gccgagagtt tgttccagat tatctttgtc aaaaattacc
     4321 ttaacatttt gaaacgtatt atcatagttg gtcaaagtaa cgtaatacag agacagcttc
     4381 ttcatttcaa aatcaagtaa gtcaaactga gtaagtacct gagttatctg acgacccctg
     4441 tcagtccgaa aattgaaaca aatcacaaca tcaccttcct gaatggtagc taccggcgca
     4501 gacttatcat cagtcagaac aaggggtttc aggaattcat ccgtaatatt tttagaataa
     4561 gaatcattaa tagcagctat tacatctgtc gtgggattcc ccttgccaaa gaccagacaa
     4621 tcataggcga gcttaactct ttcccatcgt ttatccctat ccatagcata atatcgacca
     4681 acgaccgaag ccaggcggcc ggaagttttc ctgccgtgtt taattatctg tgtgataaaa
     4741 ccaattccgc ttttcggatc cgtgtcacgt ccatcggtga atgcatgaat aaaaagattc
     4801 ttgattccct tttttgcggc aattgtcatc agccctttca ggtggtcaat atgagaatga
     4861 acacctccat cagacactaa cccgaaaaga tgaacttttt tgtcattggc ttttgcataa
     4921 tcaaaagcgt catttaagac ttcgtggttt tcaacagtgc catcttctat cgctttggat
     4981 acttttacta aatcctgata aacaattctt cctgcaccaa tgttgaggtg gccaacctca
     5041 gaatttccca tctgtccgtc cggcagacca acatccaaac cggatgttct taatgtacta
     5101 tgcgggcaag tttcgtataa ggaatcaatg aatggcgtat tcgcctgatc aatcgcggag
     5161 acctttgtat ttttggcaat tccccatcca tccagaataa tcaaaataat tttccttgcc
     5221 ataaaattta gtaattcttc gcaccgcaaa tataaggttt tagtgaggaa cattcaggag
     5281 ctcgtttttg attaaccatc taattccata aattactgcc tttataaatt cccatgttcc
     5341 tccaattggc acaattttag ccaatgaagg gtgaagatta atttaatgaa aaaagagccg
     5401 ctgtacagat tatttttttt ggtaattttc ttcgtgttcg gctttgttgg aaaggcgcag
     5461 gaagacatta ttcatagtgt tgaatcagct atgaaattag gcagttcaaa agagcttgcc
     5521 cggtatttga atcaaactgt tgagataaac ctggaaggtg aaatctccag ctacagcaaa
     5581 atgcaggctg aatttgtgct caaagatttc ttcaagaaaa atcctcccga aggttttgaa
     5641 atcattcata atggaacttc gaagggaggt ctcaaatatg caattggaaa atattcctgc
     5701 aaaagaggat acttcagggt ttggatgaga gtgaaggaat ttagcgggaa actcctggtc
     5761 tatgaaatga actttttcaa agaagattaa tcttacttct acagcattct tccatttcga
     5821 gttaacttag agcatgaatt tgcattacct caccaatcag gctctaaatg attttattga
     5881 acttgccctc gccgaagata ttggtgatgg agatcattct tccctggcgg tgctaaatca
     5941 cgatcttgaa caaagcgctc aattgatcat aaaaggcgaa gggattttgg ccggggtgga
     6001 acttgcagaa cgaattctct tgttcaaaaa tccaaaagtt gaacttatta gaaattcagg
     6061 agatggcgac ccggtgaaga atggagacat tgcattcacg ctatccggga atgcaaggaa
     6121 aatactaagc cacgaacggc tggtactgaa ctgcatgcaa cgaatgagcg gcattgctac
     6181 ctatacgtca atgcttcagt cattaatttc ccacacaaaa gccaaagtaa ccgatacccg
     6241 taaaactacc cctaattttc ggctgatgga gaaatgggcg gtagctatcg ggggtggtgt
     6301 aaatcaccgg atgggattat atgataaaat tttgctgaaa gataatcata tagattattg
     6361 cggaagtatg gcaaatgctc ttaacaaagt gaaagattac ctggagaaaa ccggaaaaga
     6421 tttatttgtg gaagtggaag caagaaacct ggatgaagta actcaggccc ttaaattcga
     6481 aattgtcagc acaattatgc tggacaacat gccgccgtca gaaataatac gagccgtgaa
     6541 gataacagat ggaaaggtga aaactgaggc tagcggcaat atcaccgaaa gtacaataaa
     6601 ggaagttgcc gaatgtggag tagattatat ttccgttggg gcactaactc atagttataa
     6661 aagcctggat atgagtctta aggcttttta attggttcgg aatactctat tattgcgatt
     6721 ttatttttag ggaaaatgat tgttaaaaca agaaaatatc aaatacccaa gaaaacttat
     6781 ataaagttgg gattaaggaa cattatgaaa gagcagtggt gggttttgtt gattgtggcc
     6841 gccatttgca gtggagcatt tctggttcca tcaaactggt ggtttattgg tgcggcaata
     6901 ggattagtct tatacttcct tttctggctg atacaatttg ccgggatgtc gcagctggag
     6961 cagggaaaaa ttctctttga caaattgagc tatgagatag acagcaggca gatactaatt
     7021 aagataaata caaaacaggg aatgccaatc aagtgggaac aaattaaacg tgcatggatg
     7081 agcaaagatg cctttacctt tgtaatttcc aaagtccagt ttattcattt gccattcagg
     7141 attttcaacg ccgagaatca ggtaaaattt attgagacta tcctgaaaag aaaaggatac
     7201 ttaaaataac ggcaatgggt aagggtgaaa aacttaccga aagcgaatta cgtctcagga
     7261 tttcttccta ctgtgcttac caggagcgct ctccaaagca ggtgcaagaa aagttatcaa
     7321 ccttaaacac cgatgcaata attaatgaac gcgtaattaa atgggcgact gatgagaaat
     7381 tcgtttctga atcccgtttt gcaaagaatt atgtgcatgg aaaatttcat cttaagaaat
     7441 ggggccggta taaaataaaa gcggggctta aagctcacga tatttcaacc caaatcatcg
     7501 ggaaagcaat cgctcaatta gataatacaa actatgtgga aacccttata acccttggaa
     7561 tcaaaaaatt ccaatccata aaaaaaacag atcaaaacaa atggcggaag acagaaaatt
     7621 acttaattgg caagggtttt gaaacaaagt tgattcttga aaataaggag aaaatagaaa
     7681 atgggtaaaa tcctgataat tctgatgatt gcctgcgtca atccctggca aacggtaaaa
     7741 accttaaatc tcaatctgga ggatggggta gcaatccagg gatatgatcc tgtaagctac
     7801 ttttcaaaag gaggtccaaa ggaggggaag cctggcatcc gttatgtgca caatgatgcg
     7861 acttactatt ttctctccga tgaaaacaaa agcaagtttg tagcaaaccc aactcaattt
     7921 gaacccaaat ttggaggctg gtgtgcctac gcgatgggcg actcgggaga aaaggtgaag
     7981 atcgaccctg aaacgtttaa aatagtagat ggcaaattgt acctgtttta taactttttc
     8041 ttcaataata ccctgaaaga ctggaatgag aatgagaaag tgttaaaaca aaaagctgac
     8101 gaaaactggt caaaaatcac aagaaattga gctcttgtac ccttcgcaac tttaaaacca
     8161 attcccatat aaaatttgtt aaccggacaa aggaattaga tatatttgag gttacttcca
     8221 gataaaatgt tgcttctcgg aatgcgggaa ctttgattat ctggtaaaag ttaaatagta
     8281 tggaattaca aaatgcgcta aaaaaagttc tgggacagct aagagatgtt accggtcaac
     8341 tgaaagaaac ggaattcatt atgcccgtga aatctttgaa tggttcaacc attgggcaac
     8401 acacgaggca caccattgag ttttttactt gcctgaaaga tggatataag gatggaagag
     8461 tgaattacga taaacgcagt catgacacag aaattgaaag cagcaaagtt ctggcaatgg
     8521 aaatgattgc gcggctggaa gactttgtaa tgtttctgcc ggcggataag cctttcgtgc
     8581 tggaagtcaa ttacgatcct gattccggag ataatgtcct tatccactct tcttactata
     8641 gggagctggt gtataatatc gaacatgcaa tccatcacat ggcactcatc aaagtgggct
     8701 taaaagagat ttgttcagca gtgaaaatac cctctgattt tggtgttgcc gtgtccacgg
     8761 taaagtatca gaaaatccag actctgaagg cttaaaaata ttgccttgat taaggcatga
     8821 atttttcatc gaaaataata aacagcaatt ttttgatccg gttaaggaat tgggaatact
     8881 ggcccatgga aattgtatat atccccgtga tgttttactg gctatggctt tctataaaag
     8941 caagatcgcc atttttcttt tcagcgtcta atcctggaat tgaatttggc gggatgctgg
     9001 gagaatccaa aataaaaata ttgaaaaaga tcgatgatga attgaagccc aaaactcttt
     9061 acttcaatca gaatgtttct attgaggaag tattgaacaa aattgaagat gcatccctta
     9121 gctttccttt aattttcaag cctgacatcg gggagagagg tttaatggtt gagaaaattg
     9181 atacggaagc agccgcgaca aactatgtag attcaataaa aatgaatttt ctggtacagg
     9241 agtacataga tttgccaata gagttgggtg ttttctactt cagatatccc aaccggtcgg
     9301 ctggaagaat cagttctatt gtgataaagg agtttcttaa aattaccgga gatggtaagt
     9361 ctactataag aaatcttatt agaaagaatc caagggcaaa attgcagctt gatgtgttgg
     9421 aacacaaata tggagctgag atggaaagta ttctgccaat ggccaaagaa aaattactgg
     9481 tccccattgg aaatcattgc aaaggagcag cctttctgga tggcgggaaa agaattacca
     9541 aaaaaatgac acgctcattt gacaaaatag ccaaagaaat tgatggcttc tttataggaa
     9601 gattcgatct gaaatgtgag tcatgggaag atcttgaaga tggaaatatg aaaattatgg
     9661 aactaaatgg atcgggagct gaacccggtc atatctacca tccgggagct tccatctgga
     9721 acgcctatga atctctgttt tttcacatgc gggttttgtt gagaataagc atgattaaca
     9781 aaaaatccgg agtaaaatat ctgacctgga aagagggctg gtatgttttt acacaacttc
     9841 caaccttcaa gaaattgggc tttacctgaa ggatggaagc aataacactc ttcactatcg
     9901 gacttttctt cagctatatt ggctccattc ctcccggatc tataaacata agtgtacttc
     9961 agctttcaat gggaggtcat cgggcagctg ccgtgagatt caccctggtg gcagcggtaa
    10021 tagaatttgt ttacgcatat atcgccgtca aatttcagca actcatctta tcttcgcaga
    10081 tgctgcagga caatttcaaa ttaattgcag cagttgtttt gataattctg gggttgctgt
    10141 cccttcgctc ggggtcagga cagaagcgct gggagaagaa actggaatcc agcggacttc
    10201 ggaagggttt aatggtgagt gtggctaatc cccttgctat cccattttgg atagccatca
    10261 cagcttacct ggaaacgaat aactggatta gtctgaactc atccaatgaa tttatttata
    10321 tagccggaat tgcagccggt accattgcat tattgtttac gctggttttt ctgggctcta
    10381 aagcttcccg gtttatgact aatcaggaac gcgcaaaaaa aattcccggt tttgttttac
    10441 tcggattggg tatttattcg ctggtccagc ttcttattga agtagtaaca cggtaaaccc
    10501 aaaaggatct cagcgctaaa gctataacta atggcacgaa tgcggggtga agcgcttgcg
    10561 gtaatactct ccaaaaaaca agcgtaagta gtagaagtat accatagccg agaaaatgat
    10621 atacaatcaa tttcggtttt ttgtttttaa tcagaaaata aaggaaaata ccatgaaatg
    10681 gattcgccca caacaaatta aaattccagg cggatttgtg ttcagttcca aaccaaagaa
    10741 taacaagaaa aattcccagc aaccaaactg gactaaaaag aatgacatct ataagtttga
    10801 ggtgcctgcg tttcagaatt tcccataaag tgaaaccggc aactaccagc agcaaaagcc
    10861 agaaaaccgt tttcggagta aaaaaaccca aattatattc cacgggtttt gcttcaaaaa
    10921 ttaccctctt atctttaacc agtggaacaa ttgaatctcc atgaagtatt gtgccattat
    10981 ctaccgcttc gtataagtat tcaggaagaa acaaataatc ccccggctgc atgatcttgt
    11041 caatttctat ggccagacct aaatcgattc ccaaatcacc ccatggctgc aggggtgtat
    11101 atacattcac catatccctg aagctccggg cttcggtgac atatgttatt gtatcgagat
    11161 aaattctatc tccaaaaaca tgaaaaaatg catccggtat gcgggttgag caattatcgt
    11221 aaaagtaatc atagaggtaa tccttgtttt caggaagaga attccagtta agaaattcaa
    11281 acaacgtttg tcgctgtgat aatgtgagat ttaattcctg ctggtagatt gactggttga
    11341 aatacatgta tacatattcg gtatgttcgt attttccgat agccagagaa taatagggtt
    11401 caccaatggc aaaattcaga tagaaatttt tctgatcgaa atcgaaagtt ccataattat
    11461 ataccagatc aattcctttt gatgcatctg taatccggat agcactatga ccaaagcatg
    11521 tatacacctg ttgttgcccc ggaccaaaag tgagcataaa tacttttgat tgatccgaaa
    11581 gacgagcctg tcccagggat ggaacggaca aggtaaatag cagagggatc aaataatttt
    11641 tcatgcacaa atggagccat tacaaggtta acaaatattg taacaaaact atagatgata
    11701 attttgtaat ttcggcattt aatgagaagt ttttcggatc gcttaaatct gttatctaag
    11761 acctcacccg gtaagttgat taatgcttcg cgggtgcttt caagttatta catttccaga
    11821 attagcggta tgccaaatca ccggggtctg ccattcagca tcggcatcga gccaacaacc
    11881 tcatgcaacc tgcgctgccc ggaatgtcct agcggacttc gttcgttttc cagacccacc
    11941 ggcaatctgt catcggatac tttcacgaaa gtaattgatc aactttccac ccatctcatt
    12001 tacctcactt tctactttca gggtgagcca tatcttcatc caaaatttct ggaaatggtt
    12061 agctatgcat ccggaaaagg aatttatact tcaacatcaa ccaacgctca ttatcttgat
    12121 gaaacgactg ccaggagaac agttgaatcc gggcttgaca ggataataat ttcgatcgat
    12181 ggagccgacc aggaaacgta tcagcaatat cgtgttggag gaagttttga aagggtgata
    12241 aatggaacaa aagaattatt atccgccaga aagaaatcag gcaagatgaa acctcacgtg
    12301 gcctttcaat ttctggtagt aaaacccaat gagcaccagg taaccgggat taatagattg
    12361 ggggaaaatt taggtgtgga tgaaattttg tttaaaacgg cccagattta tgattttgaa
    12421 aatggttccc cgcttattcc ggatcagagt aaattttcca ggtatcataa aaccaaaaac
    12481 ggaaactggg aaatcaaaaa ttcattggaa aatcattgct ggaagatgtg gcattcatgt
    12541 gtgataacct gggacggcgc ggttgttcca tgttgttttg ataaagatgc cagccattcc
    12601 atgggtaata ttaatgaaat gccgtttaaa gaaatttggg aaggggagaa atacaggaag
    12661 ttccggcaaa gccttttgtc ttcacgaaag gaaatagata tctgtaagaa ttgtacggaa
    12721 gggaccactg tctgggttta aatccatttg ttctttatta tttcaagtac aagcggatac
    12781 acattgccgg cagcaattat ttccctacca aacaggaaac tatttccacc cgaaaaatca
    12841 gagaccgttc cacccgcttc tttcaccaat agggtccccg cggcaacatc ccacgggttc
    12901 aggttatact cgaaaaaagc ttcaaatctg ccgcaagccg tataggcaag gtcaaccgct
    12961 gcacttccaa ttcttctaac tccatgcgtc ttccgcatca tttcttccag gatttcaaaa
    13021 taggttccgg ttttgccaaa atcatggtaa ggaaatccgg ttgcaatcag gctttcctca
    13081 agcaatttac ttgcagaaac ttgtattggc tttccattaa gaaatgcgcc tttatcctta
    13141 atagcgtaaa aacactcttt cctggttatt tcatacacta cacccaggta aacttcttcc
    13201 tcgaaagcga gtgcaacact gattgaataa gcggggatat tgtgaacgaa gttggttgtt
    13261 ccgtcaaggg gatcaataat ccactgccac tcactttctg attgtggcac agttccctct
    13321 tcgaccagaa atccggcttc aggtgctaat ttaccaaggc cctcaattaa cttcagctcc
    13381 gcctgtttgt ctacataact tacaagattg ttgaaagact tcaattcgac ggcttcgtaa
    13441 ctgaatgttt ctctttcttt aagaatgaac tctccaactt ctttaaccaa ttcgataacc
    13501 tctttttcta tattaatttc cattttttga aagataacct tttaccatca atgaaagtaa
    13561 aatgaatatg ctcagccaaa agcttgtctt tcccaggtat tctccataat ccaggtagta
    13621 agaataaaac gggtttagcc taaaattatt ccaataaacg gagggatttt caaagaaacc
    13681 ggtcaaatct ccttccgggt tgcctttaaa gacgaacatg ctgattatca taggtagaat
    13741 tgccaccccg gaactaatca gataccgggt gcgaataatc ttgcttagaa attttccttt
    13801 ttcatcaaaa aaactgtgag tgaaaagaat attagtgagt aaaagccaaa aagttgtacc
    13861 ctggaaacct gtaaatcgta tccaaaccat tgattcaccc cataggcgga atgccgcgcc
    13921 tggcaagtgg ttatataccg ggggtgaaat ggcaatggag atatattcta ctcccaacca
    13981 gaaaattacc aggtaaaaac ttttcaatct gcccaatctg gttcccgaat aaaaccacaa
    14041 caaaaatgtg agggcaggag atagacttgc aacaaaatcc cgaagcgagg gcgtaaaaac
    14101 caggaatttg aaaagagcag gaataaaaaa agtgaagaag ataattatta ggagttccaa
    14161 atagaactta atctttacct cattcaggta caggcaggcc gctataattg gtacgtacgc
    14221 aaaaaactga aaaaaaggaa atgggctgat agtccatgcc aatgtctgaa gaattactgc
    14281 ggctccggaa agcttgagta aatatctgta atcaagttta tcggttccag ctgacaatct
    14341 tttccaattt gccgggatct aaaattcgaa tttttccact tttaatttct atagattttt
    14401 catccttgaa atctgacagc gtgcgaatta cagattcggg tgaggtgccc acaatattag
    14461 ctaaatcttc ccttgaaata gttaaggttt caccaggtgt ggtgctgtta tatttctctt
    14521 ccaggtttat caatgcctcc gcaacccgct gccgtaccga attatacgcc aggtgcaaca
    14581 tttgttcttc cttttcctgc aagttatcag acagtatctg gataaatttc cgggtaacat
    14641 cccggtttgt gtacatcaaa gattcaaagt cttgtttggg tattacatac agttcggaat
    14701 cttccagggc ctcggcggac tcagaataag aagtattagt aataagcgca ttgtatccca
    14761 aaaattcccc ggtagagtga actccaagta ttaaatcttt tccgtcttga tttgacttga
    14821 aagtctttac tcttccggaa cttataaaat aaagatggtt aggatagtcc gtctcatgaa
    14881 aaacagaggc ctttttcttg aacatcctga gcttgtgatt acttatcaac atatcaatat
    14941 ttgacactgt tgcagcatct tgaataaatt cattaatacc ttccacggag cgctggaaat
    15001 tcttcttcat taaatctgat tttctcaacc ggctctcaat ggaatccagt aattccgtat
    15061 catcaaaagg cttggtcaga taatcgtcgg ctcccaggtt catgcctttt ctcaaatcag
    15121 cgcggtcggc tttcgcggta agaaaaataa agggaatgcc ggcggttttc tcattcttgc
    15181 tcagaatatg taaaactcca taaccatcaa gctcaggcat cattatatcg cacacgatta
    15241 agtccggcat atttttctga gcgagttcaa ctccggcacg gccattattg gctgtcatta
    15301 ccttgtagtt agccaattcc aaaatttcgg cggtattttc gagaacgtcc tggttatctt
    15361 caattagtag tattgttttc atcgtcggtt aagttattaa gtaattttat cataaaggta
    15421 gatcctttgc caacttcact tttgaatgat atggaaccac caagcaaatc aatgtatctt
    15481 tttacaagat tgagcccaag cccggttccc tggatatgga tggagttatg tgctctgaaa
    15541 aatctcttaa agagatgaat ttgatcttct tccggtattc caattccata atcttttact
    15601 tcaatagtaa cacttgattt ggaagttaca actgaaactt caattgggtc tccctccggt
    15661 gaatatttta ttgcattcga aattaagtta tttagaatat ttttcagaaa ggatttatca
    15721 ataaataatt catacgttcg cacattgaaa ctgttagtaa tgatttgacc tctccttgtt
    15781 atcgttttca attctgcaat gatttctttt ataaatgaga ttaattcaat tttttcgtgt
    15841 ctgctttcca cctgcccggc ctccagctta tccaaagaca aaaagtcatt aagtatgccg
    15901 gtcatttcac taacattatc ttttattcta tttatatgtt taatcctttt ttcattcatt
    15961 tcttctttgt catacttacc aataagggat gctgaagaga ggatcgtact taacggagta
    16021 cggaattcgt gtgaagcaag tgaaacaaac cgggatttca tttcgttgag ttctttttcc
    16081 ttctctaagg caactaaagc ttctttctca gcccgcttgc gttcttttat ttcacgctcc
    16141 agttcgttat ttattttttc cagattttgc actgactcag ctaattgttc ggttcggttt
    16201 tggacccgcc gctccaattc agttgcatag actattaatt tttcttcact attctttagt
    16261 tccagttcag catttttgcg cactgatata tctatgacaa acgcgatggc aaacataact
    16321 ccctcaattt caaaatggct caagctcacc tcgataggaa attcggttcc attcttcctt
    16381 aaacccacca ggtcacgacc tgcccccatt actctgggtg ttggattctt atgaaaagta
    16441 ccccgcattt ttatatgatt cgttcttagg ttagtaggta tcaggctctc cacagacttc
    16501 ccaattaact cattactccc gtatccaaat agagagtggg aggcaggatt tgcatctgca
    16561 ataactccct tttgattggc aagtaatatc ccttctgtcg ccgatttata tatggctttg
    16621 aacgcgtcgt cgctttcgaa aagcttcttg tatgagtcct ttcttcccat attggctaat
    16681 ttacttagaa aagtagctaa acttttccca gtgacaaaat acactcccga aactgattaa
    16741 tgtcataaga taccatcacc caacaaaata atttcgtacc atggccagtg aatcacaatt
    16801 tcacaaaagg attgttagcg atattcttgt tcccgtagat ttctctgtta actcagataa
    16861 tgcactgatt tatgcaataa atattgctcg tcagtgcaat gccaaattat ggattctgca
    16921 ctgctacagg ctcattcaaa cagttccgga aatggaaggg gttgcagggt tcagccttaa
    16981 agaggttctg gaaaaggcct ggaaggatga gtttgagaag ctgaaaaaaa attattttca
    17041 tgaggatttc accaactatt cctttctcct ggaaataggg tttccggagg atgtaattaa
    17101 acttcaaacc aaaaaaatac caatagatct ggtaataatg ggtacagcag gtgcaaccgg
    17161 aattggcgaa attctgggaa gcacaacttc gaaggttgtt gacatggttg attgccctgt
    17221 tctggcaatt cctgcaagcg ccagattcaa tggaattaag aaagtgacta tcgcttacga
    17281 tgaagagaag gtaaatgatc ccaattcgta tagcatgatt ttgaatttgg ttcggaagtt
    17341 taaatcgaaa ttggatattg tgaactttaa tactaccctg aaaacaggcg gaaaggaaag
    17401 tgagggaagg ctgtctctcg atccttatct ggatggtgta aaacataaat atcatttcaa
    17461 ggctgccggg aaaattgaaa aagcaattac agattttgtt gaggcagaag gaactgatat
    17521 tctggtaatg gttgctcatg atcatggatt cattgaatct cttttccatt caagcataac
    17581 caggaaaatg gtgcttcatg ctaaaactcc tttattaata attcatgaat aattaaattt
    17641 taaatagtat agcaatgaaa aacatacttg tcccaacaga ctttagcgaa tacgcgaaca
    17701 atgcttttga ctttgcgtgt agcatagcgg aacattcaaa aggagaagtt acactattgc
    17761 acgttctcga ataccctgtg atgtctgtat ttaacaccac cggtgagatc tctatggatt
    17821 caattacgga tgcttacatc gtgaatttga aaaagcgagc agatgaagaa ctagacaaga
    17881 agcttgtaaa tccagcatac cctgatgtca ggataaagaa gcacataaga attggcaatc
    17941 cttatgagaa tattgcgcgg ttaattcagc aatctgatgc agatctggtt gtaatgggta
    18001 ccaaaggagc aagcggactc agggaattat ttattggatc caatgcagag aaagcagtca
    18061 gattttcaaa atgtcctgta atcactgtac ccggcaaatt tgatttcaat gccatcaaaa
    18121 aaattgcgta tggaattaca tttgaggata atcataatga agcattattt gaactaaaga
    18181 aatatcttca tttgtttaaa gcggaattgc acctggtttg ggtaaatacc ctatacgata
    18241 agatcactga cacagaggcc aggtcaaaga tggatcagct ggcaaaattg catatgttgc
    18301 caaattattc agtaaattgt tacaaaagtt ctgctgcaga tgacggcctt ctgttttatg
    18361 ctgaagaaaa taatattgat atgattgcat tggcaacgca tagtcggaaa ggactcgcac
    18421 acatttttgc cggaagtttt gctgaagatg ttgttaacca ttctataaga cctgtttgga
    18481 ctttcagctt gaagagtgaa aatttaaaag ctaaaatcaa tgaaaagcaa aagcaatatc
    18541 agtaaaatac tggtacctac ggatttttct ccggaggcaa ttaatgcgat gacctttgct
    18601 gcccaggtgg ccagattaac ccaggcagaa gtcaccttgc tttatgtctt aaattacccg
    18661 ggggggtccg cctttgatac gatgatgtct gaatatgaag atccgtcgaa gaacaaatat
    18721 gttcaggaag ttttgaaaga tgttaatgag gtttttcaga aatatgagat ctccaagact
    18781 tttaaagatg tgaaagtggt gaagaaaatt cagattggga atcccttcac taacatctct
    18841 gatgaaatct cacagaataa gacggacctg gtggttattg gaacaagcgg cctgggaaat
    18901 gcgaggaata acaatttgat tggctcaaat accgagaaag ttgtccgccg tgccagttgt
    18961 cccgttattt ctgttaaaga agatattgat ttaaagaaat tgaaatctat tgtatttccc
    19021 acaacctttg aaaaagaatc agattcgatg gctttatttc tgaaagattt ttccaaaatg
    19081 attaaagcgg aagttaaaat ggttttcata aatacgccgg catatttcaa gactcaacac
    19141 atgattgaga aagcgtacga aaaatatgtg accaaaaata agataaaaga tttcttcttt
    19201 atggtttaca atgagttaac ggaagaaaaa ggaatccttc attactctgc ggatattgat
    19261 gctgatatga ttgctcttgc aactcatggc agaaaaggtc tggctcatct gtttgcggga
    19321 agcatcgcgg aggacttaac ccgccatacc aaaagaacac tattaacgct caaaacctgg
    19381 aattaagatt caacacaaaa ctttaaacca aaaaagacta tgaaaattca agcccccgtc
    19441 tcccaaataa tgactcctga acctatctac gcagaagctg acaatacttt tagccaggcc
    19501 agagacctgt ttttgaaatt taaaattcac cacctgccgg ttgtcaagga tggtaatcct
    19561 attggcataa taagtaccat cgacatacta aaggcttaca cagttgccgg gcaaaagaaa
    19621 tgggaatgtg atgatgtatc aatgaataag aaactttcga taagcgatgt catgaccgat
    19681 gatcccgtga atatttctcc tgccacatcc cttcaacagg cgatcagggt ttttcaggaa
    19741 agcaatttcc aggcattgct tgttgttgaa tttggaaaat tagtgggaat tttaacattg
    19801 aaagatatcg tttcccatat tacagactaa ttccatcaat gtcaatttca aaatcttcgg
    19861 gcttattcag attaacgctc tttattgagt gaaacccgtg ctaatggaaa ggaaacactc
    19921 agaagaaaga ttacataaag taaaagccgg ctttggcaaa ttcactaatt atttaagaga
    19981 atttgtttat ggaggcatgg atggtagtgt aacaacattc gcggttgtgg ccggagctga
    20041 aggagcgaat ctgggatcaa cagtaatcat aattttgggc ttcgcgaatt tgctcgcaga
    20101 tggtttcgcg atgtctgtag gaagctactt gtcctccaag tcggacaatg aaaattatga
    20161 gcggcataag aaaactgaat actgggaagt agagaatatt ccggaggccg agcgagaaga
    20221 aatccgggaa atttaccgcc aaaaaggctt tgaaggcgaa ttgttagaag acgttgtgcg
    20281 ggtaattacc gcagacaagg acagatgggt aaatgtgatg atgaaggaag agctggaaat
    20341 gattccggaa aataaatcac catttgctat gggggctgct acattcattt ccttcgttct
    20401 ggtagggtta atacccttaa tgtcttacgt agcggattca tttatttcac ttaacaacac
    20461 ctttgcaatt tctgcaattc tgacaacgct tgctttcgta ataataggta tggtaaagac
    20521 gaaagtcacc caaacttcaa tttggaaggg agtaatggaa acgctcattc tgggctgctc
    20581 agccgccgta gtttcctacc tggtaggatt tttacttgaa agtttcgtcc catagcgtaa
    20641 ttcaaataat ttttgtaaat tagtatagat gcctggcggc atataattga ttccggtctc
    20701 ctgaaacaag gttgccatga ttaatgtcat gttcttgctt gatggggatc atttttgggc
    20761 gaacgcgacc ccgttatttt taatccttta atcattaact ctgtgatatg aaaaaattac
    20821 ttctaaacgc ggcattaatt attgccgtaa cctttctttt tggcaattgc gggggctcgg
    20881 gaagtgaaac tgaaaatgtg gaaactactc caaagccaag gcttgccggc gaagatgctg
    20941 accccatgga aaataaaggg attgggcctg taaagagcct taatttgcct gatgcgatag
    21001 atcaggcaat ggctgccgaa ggtgcaataa tctatgttca atattgcaca tcctgccata
    21061 aaccgggtga gaaatttatc ggacctgctc cgaaagatgt cctcacccga cgaagtccgg
    21121 aatggataat gaacatgatc ctgaacccgg aacaaatggt aaaggaagat cccattgcaa
    21181 aaaaactcct ggttgaatat aatatggctc ctatggccaa ccagaattta accgaagaac
    21241 aggcgagaaa aatcctggaa tactttagaa ctctttgaat taactaacca ataccagtat
    21301 gaaaacaaat caattttatg ccgccatttt aattacatcc ctttccatgc tttttgcctg
    21361 taacggcgca caaaaaggtt ccggagcttt aagttcagat gccgcttcgc gcgtgtatgt
    21421 tgctccggga gaatatgatg aattttacgc cttcctttcc ggaggtttca gtgggcaagt
    21481 aagtgtatac ggccttcctt cgggcagact cctaaaggtt atccctgtat tttccgttga
    21541 cgcggaaaaa ggatatggtt ttaatgagga aacaaaaccc atgctgaaca cttccttcgg
    21601 atttattccc tgggatgatt cccaccaccc ggaattatca caaacaaatg gagttcctga
    21661 tggtcgctgg ttgttcatca atggtaataa tacgccaaga atagcaagga ttgatctcac
    21721 aacgttcgaa acaaccgata ttattgaaat tccaaatagc ggcggtaacc atagctcacc
    21781 tttcactacc atgaataccg attatgttgt tgccggaact cgctttagtg tgccaattcc
    21841 gcaggaagat gtggctataa gtgagtataa aaacaaattt aaaggtgtcc tcagctttat
    21901 taaggtagat gatgaatcca tggatatagc tttccagatt cttgtgcctg gctttgatta
    21961 tgacttggct cattcaggaa aaggtaagtc tgatggttgg acatttttca ccacttacaa
    22021 ttctgaaaaa gcgcatacac taaaagaagt aaatgcctct cagaatgata aagattttat
    22081 tgctgccgtt aactggaaaa aagcggaaga atatctcgcc caggggaaag ccgtagattt
    22141 tccaacttct tacgtagtaa atcgatatag tgagaaaaca catatggcaa cttctgaaac
    22201 cagaactact gtaaaagtgc ttctcgcgtc agaatgtccg ggccttattt actatttgcc
    22261 aactcctaaa tctccacatg gtgttgatgt tgatcctacc ggtgaatata ttgtgggaaa
    22321 tggaaaatta agtgctgatt taactgtgca ttcctttgac aaaatgatga cagccataaa
    22381 taatgaaagt tatgatggag aaatagatgg aatacccgtt cttaaatttg atgaaatagt
    22441 agccggtatt gtgcaaagcg gcggcttggg tccattacat acggagtttg atggcaaggg
    22501 aaatgcttat acatcattct tcatatcttc tgaagtggtg aaatggaaat taggaacctg
    22561 ggaagtactt gatagagtgc cttcctatta ttctatcgga cacctgatga tccctggtgg
    22621 tgattcaatg aaaccatggg gtaaatacct gctgtctttg aataaaatta caaaggacag
    22681 atacctgcca accggtccgg aattaaatca atcagcacaa ttgattgata tttcaggcga
    22741 caaaatggaa atcttacttg atttcccaac aattggtgaa ccgcattatg cgcaggcgat
    22801 agccgccgat ttggttatgc caaactctaa aaaaatctat aatctgaaag aaaatgaaca
    22861 tccttatgcc gttaaatctg aaggtgaaac caaggtggaa agaaagggaa atgaagttcg
    22921 cgtttatatg acttcaattc gcagccattt tgctcctgac aatatcgaag gaattatggt
    22981 tggggataaa gtttatgtac atctaactaa ccttgagcag gattgggata ctccgcatgg
    23041 gctaagtatc atgggagcaa ataatgctga attgctggta atgcctggtc aaacggaaac
    23101 cctttattgg gagccgcaac ggcctggagt ttatcctttt tattgtactg atttttgttc
    23161 agcacttcac caggaaatgc aggggtacat tcgcgtttcc gaacgcggtt ctaacacaga
    23221 gttaaagtgg tccctcggca attaggtgat ttggttgcaa aggggtcttt cgaccccttt
    23281 tgcttgttat cccttatctc atgaaaaaaa taaatctctt ccagcttgtc gcctcattct
    23341 tgctgtttgc tttgtttata tttccgctct ggaaaattac tttaactgca cctcaatatc
    23401 cggaaggaat ttctatgtat atctggataa acaaaattac gggcgataca gaatatacct
    23461 tgcaaaacat aaatattctg aatcattacg tgggtatgaa gtacattgtg cccgatgaat
    23521 ttgccgagtt taaatatttc ccaatcattg tggctggaat ggcagtcatt ggaatgattt
    23581 tgtcattctt taaaaacaaa aagttagcaa ttggctggct ggtggtttta ttgttcatgg
    23641 gatgcctggg tatctatgat ttctacctgt gggagtatga ttacggtcat aacctgagcc
    23701 caacagcgcc tataaaagtt cctggtatgg cttatcaacc tccattgctg ggatcaaaaa
    23761 tgttgctgaa tttcaaagct gattcatatc cgtattgggg cggccttttt cttggaataa
    23821 ccatagtgct ttcaactttt tctatattca aaaagaataa aagatgaaag agctattttt
    23881 aactctttgt tgtctcgctt ttttatcttg ttcttccggt cctcaaccca tagcttatgg
    23941 aacagatggt tgtcattatt gtaagatgac aattgtagac aataaatttg gcgctgaaat
    24001 tgtcacaaga aaaggaaaaa tattcaagtt tgatgcggtg gagtgcctgg tgaactattc
    24061 aaagcaaaat ccaaagcagg atggggatac ttactacgta attaattatc ttaatggtga
    24121 atggattgtt gcggagggag ctgcttacct gagatccaaa aaaataccaa gtcccatggg
    24181 gcaatatatt gcttctttca gtgcacggga agctgcgaaa gatattcaaa atgaaaatga
    24241 aggggtaatt caaacatgga atgagttgat tgataatttt gaaattacta aaatagagaa
    24301 cttctaaagt attccggttg aaggtttggt ctatgatatt cctgacaatt gcaggagcat
    24361 gcaaattaca tgcttcaacc actgaggttt gttcaaactg ccaaattaaa acaatcactg
    24421 aagcaattag aatttctcgg cccggtgata ctctgcgcgt aatgaaagga atctataaag
    24481 aaggaaccat aacagtcgac aagccgattt acctgcaggg aatagactgg cctttgttgg
    24541 atggcgagaa taaatttgaa ataatgcgaa tcatttcgga taatgtaaca gtgatgggtt
    24601 ttcgattcag agatgttgga gtcagttaca tcagtgataa tgcggcgatt agtttggagg
    24661 cagtaaagaa ttgccggata gctaataata agtttgagaa ctctttcttt ggcatttacg
    24721 cgaaaaattc gagtgaatgc gacatagtaa acaatgaatt gatcggcaat gccttggtgg
    24781 agatgtcaag cgggaatgca atccatttat ggtacagcaa aaagatgatt gttaaggata
    24841 acgtcgtgaa gaaacatcgg gatggcattt acctggaatt tgtagatgaa agcattgttg
    24901 agggtaatgt cagtcagaac aacatccgct atgggctgca tttcatgttt tccaatcatg
    24961 ataaatattt gggaaatact tttagcaata atggtgcagg ggttgcggta atgttttcgc
    25021 gaaacattga aatgactaat aacacgtttg aactgaattg gggaactgct tcctacggat
    25081 tgttattaaa agagatttat gatggtaaaa tcataaaaaa taaattcctt tataatacaa
    25141 taggtattta tggtgaaggt gccaaccgga ttgaaataat ggaaaatgaa tttatgggaa
    25201 acggctgggc gctgaaaatc ctgggaagct gtgcggataa taccatttct aaaaataact
    25261 ttatttcaaa tacttttgat ttatctacaa acaattcccg taattataat aactatgatg
    25321 gaaactactg gagtgactat tcgggttatg atctaaacaa agatgggaga ggggacattc
    25381 cttaccggcc aatgaaatta ttttcttatt tggtaagccg ggtagagcct tctattgttt
    25441 tgctgagaag cttttttatt gatattattg attttgccga aaaagtaacg ccaatgttta
    25501 ctccaccaac cttggtagac agtgaacctt taatgaagcc ctggaaaaat gttgcagata
    25561 attaacttac acaagagctt tggcaaaaac caggtcataa aaggcgtgga cctgtccata
    25621 gagcaaggaa aactcaccgc tatattgggg ccaaacggct cgggtaaaac cacgatcata
    25681 aaaagcattt tgggaatggt aatacccgac aaaggagaaa ttatttttga tggtgagaat
    25741 attaaaaagc attggaacta ccggaagaaa ttaaactatc ttccccaaat tgcccgattc
    25801 ccggaaaatc ttcgggtgaa agagctgctg gccctggtta aggtactccg gaggcaggaa
    25861 tctgatgagc aggagatcat tgacaggttt gctcttggaa atgatttgga taaaccactt
    25921 agaaacttgt cgggaggtac ccgccagaaa gtaaatctcc ttctgacgtt tatgtttaac
    25981 aaccccatga ttatccttga tgagcccagc aatggattgg atcccgtcgc tctgcttgct
    26041 ttgaaagaat tgatttcttc cgaaatgaag aagggtaaaa ctatcttgtt caccacgcat
    26101 attatgagtc ttgtggaaga tcttgcggaa gagataattt tcatattgga aggcaaagta
    26161 tatttccagg gaactcaacc gaagctaatg gaattgacat cggagcgcaa tctggaacga
    26221 tcaatcgcag caattctaag ttcgaacgga gttaacaaac catgaaaaag tattaagaac
    26281 tgatggtcaa aatatttaaa tacagttttt cagatttaat ccgaagccgg tggagcctcg
    26341 catatttcgg attttatttg atcacggcgg tcgccttgct ttacttaagt acggatctat
    26401 cccgtgctgt tgcaagcctg atgaatgtag taataatact tagccctttg attggaatgc
    26461 ttttcggggt gatgtattac tataattcaa gggaattcat tgagcttttg ttggctcaac
    26521 ccattaaaag atcctcaatt tttctgggtc agtattttgg attggctgtt tccttgtccc
    26581 taagtttctt tttgggtatt ctgcttcctt ttattttcat tggttcggga gaaatcaaaa
    26641 tctggttgaa ttttattaca ctgattgtct caggagtatt tttaactttc attttttctg
    26701 cccttgctta ttttgtttca atacgacatg aaaacaagat aaagggattc gcaacggcca
    26761 tcgcgctttg gctttttatg gcggtaattt atgacggaat tttcctgttg ttgcttgtgt
    26821 ttttcaatga gtatccaacc gacaaacttg cattgggaat agttatgcta aaccctgttg
    26881 atcaggcgag gattctgatg atgcttaagc tggatatttc tgcaattatg ggttatacgg
    26941 gtgccgtttt tcaaaaattt ttcggcactt ctttaggcat gacaatgtct ctgttaatgt
    27001 ctgccctttg ggtaattttt ccggtttggg gaatgatcag gtacgcgaat agaaaagatt
    27061 tttgatttga aaaatttata aattgccctg aataaaatct gattctccaa tcactcaata
    27121 actactttgg cgaaaaaatt tgcaaacctg cctgaaacag tgatgaggta aataccttta
    27181 tttaaatgat gaagatccaa ttcaaaattc ctttctaacc cttgaacctc ggtttcaaaa
    27241 acctcaactc cggccagatt taagacctga attctttttt caccgggcac ctgtgagggt
    27301 aattcaatcc tgaacctgcc atcagatggg ttaggccaga tattcattga agtattttca
    27361 tagttaatgg ccactaccgg aggggaggtt ccgtcactgt ttataatcag gacaccgcca
    27421 ttgtcagctg ttgcaaaaac tccgggagtt aggctcaaat ttgtctcggt catatcaatg
    27481 tcaaattgac ttcccggttc gaacccgctc aaataatatc tcttaactcc tgcaattctg
    27541 ttgtgtgtga atccaacgaa attctgatta gggtttcgtg cgaatagaaa ggccagtgtg
    27601 tcaatcctga ctccatccat ggggaagtct ccattccatg atatcaagtc cgctgtcact
    27661 gcctgggggg catcctggcc aattagcaaa ttcaagaacc ggacactatt cggattttca
    27721 ttagcttcaa tcattagccg ggttggttgc aaataatcat cattttgtgt ttgcatagga
    27781 tcataaaccg acaggtaagg ctcaatatcc aggtcttcat catttgtaac tatttgaatt
    27841 gttgaagcgg gcggcagcat gttcatgatt ttgagattct gaccgcctgg tgttaatgca
    27901 ttaatttctt ttcctgcaat ctccggattt gcatcgggca ggtttagcca gaatcgctta
    27961 aacccattgt tagtggtttc tacccgatcg taaatgatta tgagatcggg ttttagccag
    28021 aaaatggatc gacctgcatg gcttacattt ctcaggatgg gtctgaatcc ggagccgtca
    28081 tcataattat aaaggtttgt agaatttccg gttacaaaaa caaagtcatc atttattgtc
    28141 attgcttcaa taatcggatc accgtcgcta ttcgccaggt tatactgcga cccccgcctt
    28201 gcatgaatat tcccgatagt gcccagatgt aaaccatctg tttcgctctt gattgtcaga
    28261 gtattcttat agtcggagct cgcggcaaca ttgccgtaac cggttctttc tttggtcagc
    28321 cattctcctt ttctgaaaaa atcaaacata tttccgtctc cgtgctgatg gtcaatccct
    28381 gtccagccaa gattaaagtt aaaccagctt gcactctggc tccagtcggt tctgcttaaa
    28441 attctaccag gcccggctgc aaaataatgg gttggaaaac tttcatgtgg atcgggcaag
    28501 gtattgtttt gggcttctat tgcagattca gggtcgaata tcatgaagta aagaatggaa
    28561 ctgttcattt cttccttact tcgcactctg ctaaataacc tcgcttgccc tcccggagct
    28621 gcataagttt caatccagcg aattgcatta aggagacctg tattaccttc aattctcgca
    28681 taaaccccaa gcggaccaaa tattctcata aattccggag caaaaaaatg ttcaccgtct
    28741 ccataccagg ccatctggta ataggtcccg gaacggctag gtaattctac ggaatttgga
    28801 gaaagactat taagtacacc tgtgacagta ctttcccaat gggaattcga attaatggaa
    28861 actttagcgc cccagggggt tcctgaagat cctgtatcct ccggccggta tccagccgta
    28921 tataatgaaa gaagaaggtg taaaggatat cccattgatg gcatatactc aattccttca
    28981 gcgcttagtc ctccgttgtt ttcatgccga aatgcataat cggtcataaa gtaccaggtg
    29041 tggaggattt tttcacgata ttctcttagc cggccatcgt aatcatttgg aagtgcaggg
    29101 tctgcaatat catcaccggc atcgaaactc atggccatca tgaccgagtt tctaatgagc
    29161 atcatgaaat aattattcat ggcaaatcgc agggctctct tctgcggatc atttaactca
    29221 agccaggctt cattattgta attggcaatt tcaggaaata aatcaggtcc gttacgaaag
    29281 taattcgcat gcgggtaagc caccatgttc atttcaatcc accggatgaa cacccttctt
    29341 atccttgctt tttcagcagg ggagaatacg tcataaatcc agtcgacggt caaggggaat
    29401 gcgaggccta tccatcgtgc acggtctgag gaggaaaaat tatgccggcg atagcggagt
    29461 tcgtcagttg tatagttagc ttcctctccg gatgggatta tgccattgtc agcccggtca
    29521 attatataca tcaagagtga ttttgctctt gctttataat cagcttttct ggaggcgtca
    29581 ctttcaacca gagacatgaa cgcgagaaca tgggcaaaac cttctgtata gtaggcagag
    29641 tacgctgaat ttcccgggtc ttgttcaggc aatactccgg tatccattaa agttacggct
    29701 tcatcggcca actgcttcaa tccttctgaa tacattggat ttgacattac ggcccaggat
    29761 tgaagtgtta tcagatcatt tcctgttacg aaaagtcttg gatgtgtggt tagctcctgg
    29821 gcgagtgcta tagcaaattg gccaataaat agtataaata gtagtataaa atgtttcata
    29881 aggttaaata tatctaataa aataataaaa aaattatatg atgttaattt caaaaacata
    29941 gaataagtaa ccattttatc aggtttaatt agcctttgaa atgacttatc ttggtaaaaa
    30001 catgctttca aatggttgtt tataatgtga cggtaggcat tgataaagaa gtggaacagg
    30061 aatggcttaa ctggatgatt aatcaccaca tcccaagagt tttagctacg ggattattcc
    30121 acgaaaataa aatttacaaa gttcttaccg gcgatgaaga aaatatcagt tactccattc
    30181 aatatttcgc tgagagctta accatggtgg agaagtattt aaatgaatat gctcccgtgc
    30241 tgatacagga gcataatgaa aaatttaaaa acaggcatgc agcattccgc acattgctgc
    30301 aaagcgttga ttaatccggt ttgaaattcg ccaattgttt cctatcgaaa ttgcaattga
    30361 gccactggcc gtcaaatgtc gcgttatatt tctcatagaa tttgatggca ggcccgttcc
    30421 agtccaacac ctgccatgac atacccgtgc attgctcttc ctgggctttc ttcaccacgc
    30481 gttcaaataa taatttaccg acaccttttc ctcgctcttc ttccgttatc accatatctt
    30541 ccaggtatag ttttttccct ttccaggttg aatacctgaa ataataaata gcgacaccaa
    30601 taatttgatt cacgcgttcc gctacataca ttccgaaaac cgggttgtca ccaaagccat
    30661 cccgctccat catttcaaca gtattctcaa cttcatccaa agccttttca taggccgcca
    30721 gctctttcac caactccagc gtttttggaa gatcttcttt tttgccttct cttattttaa
    30781 ttcccataag gaaaatttag catcaaagtt ttgtttttgc aacatccggg attatcccca
    30841 tgttataaaa agcaaacgaa tataaatcag catataattc gatggctttt gaagtcggag
    30901 ttccaccgcc atgaccggct tttgtttcta ctctaatcaa caaaggattc ggtccggcac
    30961 cggctttttc ctgaagagta gcggcatact tgaaggaatg tgctggcact actctgtcat
    31021 catggtccgc ggtggttacc aataccgcgg gataagtaac atcttcttta atattgtgaa
    31081 gcggtgagta tcctttcagg tattcaaaca tttcttctga atcttccgat gtgccataat
    31141 ctgcggccca cgctctaccg atagtaaaat tctgataccg taacatatcc attacaccta
    31201 cctggggaaa agctacctgg gcaaggtctg gtcgttgatt aatagttgcg ccaattaata
    31261 atccaccgtt tgaaccacct tgtaatgcca gaaattcttt gcttgtatat ccttctgcaa
    31321 taaggtattc acctgctgcg ataaaatcat cgaaaacatt ttgcttctgc atttttgttc
    31381 cggcttcatg ccaatcttca ccatactcgc cgccgcctcg gatattaggc tgagcataaa
    31441 tgcccccgtt ttccagccac aaaattctgt atgtactgaa agaaggtcta aggctgatat
    31501 tgaatccgcc ataggagtat aaccaggtag ggtttttacc atttagatct ataccttttt
    31561 tatgaataat gaacatggga atccttgtgc catctttact attatagaaa acctgcgttg
    31621 actcataatc atcgggattg aatttaacat ccggacgttg atattcaacc gacttgcccg
    31681 tagcgatgtc gtacctgtaa attgtacccg gagtaataaa tgaagtgaag gtgtaaaaca
    31741 attcggtatc atcagcatcg ccgccaaaac caaaggcaga gccaatgtct ggcaattcaa
    31801 tgtctttgtc caggcttcca tctgtgttga attgttttac ttcggttttt gcatccacga
    31861 ggtaagaacc gaaaatcttt ccgccggcca tgctggcatt gagcacgctc tccgattccg
    31921 gaataacatc aacccaactc cctggtgctg gattggtaag tgttgtttga accacacggc
    31981 tgttaggggc atcaagatta gtctttataa atagaatttc accgatgttg ccaataatgg
    32041 agtgatcgtt ctcaaaatca tcaaccagag taatgaaatc tgaattttcc atagaaaggt
    32101 cttttacctt tagtgcatta ccacttgtgc tcgcgctggt gtaaatgccc agaaatctct
    32161 catcatcgga gacccaggca aagaacctgt tgttgggatt ttcctgatct tcatagatca
    32221 attcatcttc caattgtggt gagccaacct tatggtaata aatcttacca taaatgtttt
    32281 ctgatttcaa ttcatctcct tccccgggtt gggggaatcg ctgataaaaa aatccatctt
    32341 tataccagga agtaccggta aacttagccc attcaatttt gtcgcttagc ttctctttgg
    32401 tttccacatc catcacgtac acttctctcc agtctgagcc ggaaacggaa atcatgtagg
    32461 aaaaatattt atcatctttg gaaaaactgg taccggccaa tgaaatggtg ccatcctcgg
    32521 aaaatgtatt tgggtcaaga aaaactttgc cttctccatc cagtgttggt tttctgtaaa
    32581 gtacagattg gttctgcaaa ccctcatttc tatagtagta ataataatct cccttgtgat
    32641 tgggtgccga taacttctca taatcctgaa gctcagtaag cctttcctta agtgtctcgc
    32701 gatatgaaat ctggtcaaga tatgaatttg tagcagcatt ctggttagtt acccattcgg
    32761 cggttttttc ggaattatca tcttccagcc agcgataggg atcggctatt tgttgatcaa
    32821 agtaagtatc aaggtgatca acttttttcg ttaccgggta attgacattg gcggatttct
    32881 gttcagtttc tgaacaggag aatccaataa tcaatgcgaa aaggaataga gcaaatcttt
    32941 tcattatttg tgaattttgt aaggtttaaa attagtaaaa atcagtggat gtcagccaat
    33001 atatacagta atggtattag tcaatcttgt caaaacctgt gtaatcgacc aatacttcag
    33061 gaatggcaat tcccactgaa ctttgattat tttcaataat tgcggccata atccggggaa
    33121 gggccaatgc acttccattt aaggtatgga caagtctggt cttgccggtc gaatctttga
    33181 atcgaagttt gagccggtta gactgaaacg tttcgaaatt gctgacggag cttacttcga
    33241 gccacttctt ctgaccgccc gaaaaaactt ccatatcata ggtaattgat gatgtaaatc
    33301 ccaaatcacc cgcgcaaagt cggagttgcc tgaaatgcag attcagcttt ttcaataatc
    33361 ccatcacgta ctcgcacatc tcatccaacg ctttgtaaga attatccgga tgctgaattt
    33421 gaactatctc cactttatca aattgatgca gtcggttaag tccacggacg tgagctcccc
    33481 agctgcctgc ttctctcctg aaacaagggg tgtagcctac attttgaata ggtaattcat
    33541 cttctttcaa aataacatcc cggtacaggt tagttatcgg aacttccgcg gtggggatca
    33601 gatataagtt ctcgtcaatt aatttataca tctgaccatc cttatccgga agttgaccgg
    33661 ttccataccc cgaatcttcg ttcacaacaa taggaggttg aatttcagta taccctgatt
    33721 tcagagcttc atccagaaaa aaattaatta aagcccgttg caggcgggca ccttttcctt
    33781 tgtaaaccgg aaatccggcc ccggaaattt tattccctaa ttcaaagtct atgatatcat
    33841 acttctttat taattcccaa tgaggcaatg ggtcgtcaac cagatttggc ttttcacccc
    33901 acaaagttac cacttcatta tcttcagcag atttgccggc gggaaccatt ttgttgggaa
    33961 tattcggaat tgtatataag aggtctttaa gcctgtcctc aagttgatta agttcttctt
    34021 cttctctctt tgcagcctcc tttagtgtga cggatttccg ctttttattt tcagcctcct
    34081 ctttcttccc tgccttcatg agtgccccaa tctccctgga gagtgcgttg ctctctgcca
    34141 ggtgagaatc cagggaagcc tgagtcttct ttctattggc atcaagttca agcactgtgg
    34201 cgagcgcctt tttggcattg gggaaattgc gcttttcaag gccgtggatt gcattttcta
    34261 tgttttctct cagatatgga acaaacagca tggtatttga ttgttagaca ggtaaaaata
    34321 aggcaaaatt aacaaaaccg gtggacaggg gatttcgttt agaccttaag aaaaagattc
    34381 aaaatcttga ctattagtta agcttggtct aatattgcaa ggtcagtcgg attaaagaca
    34441 tcccttgtcg agattcagct aacgactttc ttcaaaaagt ttattaattc taattaccat
    34501 ttacaggagt atattcttta taataattta taagtattaa aaacccttct ttattttgaa
    34561 ctaatcattt tatgtacagt attgatgatc tgaatgtgaa acttctttca gagttgaaag
    34621 agattgcaga ggagttggga tttaagaatc acaagaaaat gtctaagaca gacctggtat
    34681 acaagattct tgatcagcaa gccctcaaac ctgatgatgc cgaacagccc ctcaaggcat
    34741 cttctaaaga aaaagaaaaa gaaaaagaca aagccgaaga cctttttgat tctttcgaaa
    34801 tggaatttga tgaaagtata acttcttttg attctccatc cgaagacgta gataaaggta
    34861 aagcgaactc cggttcttcc gatggtgaac cggaagcaaa agaaaaagaa acagaaccgg
    34921 cgtcaacaaa cgcaaatctg gaaagttctg caaaggccgt aaatggtaag gaagacatag
    34981 gtgatcgaaa acccagagga gaccttgata gccgtgataa ccgtgatagc aatgaaaacc
    35041 gtgatagacg tgaaaaccgt gatagccgtg atcaaaaccg tggcaagaaa agtggcgctg
    35101 cagctttgat aaaagagttt gacggtgtta ttgaaaatga tggcgtattg gaaattatgc
    35161 aggatggata tggattcctg cggtcatccg attataatta tctggcaagt cccgacgata
    35221 tttatgtttc tccttctcaa ataaaacttt ttggattaaa gaccggggat acaatcaaag
    35281 gccagattcg acctccaaaa gaaggtgaaa aatatttcgc cttactccgg gttgaatata
    35341 taaacggaaa gacagcggaa gaaatcagag acagggtttc atttgaatat ctcactccgc
    35401 ttttcccaaa tgaaagactg aatttaagta ccaagcctga tcggttttca acaagaatac
    35461 tggatttgtt cgcacccatc ggaaagggcc agcgaggtat gatcgttgca cagcccaaaa
    35521 ctggtaaaac cgttctgttg aaagaaattg cgaatgcgat agctgaaaat cacccggaag
    35581 tctacctgat gatcctgctg attgatgaac gtccggaaga ggtaactgac atggcaagaa
    35641 gtgttaaggc agaagtaatt tcttccacct ttgacgagca ggcggaacga catgttaagg
    35701 tggccagtat tgtactggaa aaagcaaaac ggatggttga agttgggcac gacgtagtaa
    35761 ttcttcttga tagtataacc cgtctggcca gagcgtataa tactgttgtg ccttcctcgg
    35821 gaaaaatact ctccggtggc gtggatgcaa atgccctcca caaaccaaaa agatttttcg
    35881 gagctgccag aaatgtggaa aatggcggtt cattaactat tatcgccacg gcactgattg
    35941 aaacaggttc taaaatggat gaagttatct ttgaggaatt caaaggaacc ggtaacatgg
    36001 aattacaact tgacagaaaa ctatctaaca agcgcgttta tcctgctatc gatgttcctg
    36061 cttccggtac tcgtcgtgaa gaattactca tggataagga agaattacag cgtgtctgga
    36121 ttctgaggaa gttcatgtct gatatgaatt ctaacgaggc gatggaattc ctgctggaca
    36181 agatgaaggg tacgcgaaat aatgaggaat ttcttgtttc aatgaacgga taactttaag
    36241 cctgctcagc tacaaaatct tgcagatatt gataaggcgg agttaaattt cctgcgctcg
    36301 taattgtagc tctttctatg attttatcct tatcttcttc aattagagaa ggtaaaacct
    36361 tatctataat atcacgccca aaatcctcac ttgcatttct gggtagctca gccggtaaat
    36421 tatcgattga cattacggat acgttcgctt catcaacaaa gggaagttca atccggtttt
    36481 cttcaggatt ataatcgtaa aagggttcat caatagtggt ggcttttttt gtggaaggga
    36541 tagaaccatc aatgtcacac gtgatgtctg caattacacg gatattgaaa tcaaccttca
    36601 gcatatcttc ccgggtaaat aatttcggcg cactcggatc ccaataggct gcggcaatca
    36661 gcaagtcaca ggcctttctg tacttgagaa aatcagaccc gaatctctcc ggatactgat
    36721 gaaattcctt ttttttgaat tctttgcctt ccttatgctt attgtattcc cgtggattaa
    36781 tctgactgaa aacaggttcc ttaaatcttt cattaatgaa atcggaggga tttacttttc
    36841 tgattcccat tcctaacaac acttcaatgg ctccctttgc aactctacca ccaccggtta
    36901 taagaatttt tatgttgggt aattttactt tggtgtattc agtttttagg tcatcaaggt
    36961 caaagcactc gtgagccctg cgcaatttga ataacttata ccggaggcca aatgccaaaa
    37021 ttccattata ggcgccaaca attccggcat accttccaaa tgcaacaatt ctgtttcttt
    37081 cggagtcggt caggacttcg taatcaataa gcatgatttc ttttttcaaa atttcctgta
    37141 gcaatttctt gttataggct tgtttcttaa tggtatgaga aaagaaaaaa taagttttcc
    37201 cggcgatcaa tttttcaaca ggaacttctt taaccccaag gagcacatca caatcttcga
    37261 tactattcct aacttcaatt ccggctccac ggtattcatc atccgtaaaa cagcgaatgt
    37321 cacttgactg ggcaattact ctaacatttg gatatttcga ctggagctca gaagcatggg
    37381 aaggaatcag cggcaccctg tggtccatgg gaatttttcc ttcacgtaaa agacctattt
    37441 tcataaaatg ccctgcattt aaaggtggca aagttaaccg atttcatctt aacttagatt
    37501 gcagtaatga aaatagatat tacttacatg tggctgattg gagcctgggc tatttacttc
    37561 tcacttcata gtctaatggc cagacaaaat gtgaaacaat gggctgcaat taacttaagc
    37621 cttaataaat attacaggtt agtatattcc atgatatcga caatcggctt atttgcactg
    37681 ttggtattta atggattgta tgatcaaact ttaataatct ccaggacaaa ggtaatcatg
    37741 tatctttctc tgatgctcgc cacatcaggg gtattaatta tcagccggag ttttaaggtg
    37801 tattcgctta aaaaatttct gggattaagt aaaaaggagg aaagtgaatt gcaaattaaa
    37861 ggcattctta aatatgtaag gcatcctatt tactgcggca ccatacttat tataataggt
    37921 tattggtttt ttaaccctac aattgcaacc actgtaactt gcattacaac gtttctttac
    37981 ctgcctattg gaattcttct tgaggaaaaa agcctgatcc gggaatttgg agaaacctac
    38041 ctgaattata agcggaaggt accggcgttg attccaaagt tatggagtag tagttctgcg
    38101 aattaagcat taaaaaatct catagcgcaa tcacttactt cttcctttct tcttagattt
    38161 cattttcttt ttcgttaact ttttgtcatc cacattccgg ttcaggaatt catttatttt
    38221 gtcgatatcc atagttgatt tcaactcacc gaaggagtca acttttaatt cgaacccttc
    38281 cagttgggga ttaactctgg gttttttttt agattttgcc acaagtctaa tttatagaat
    38341 tgaacgtggt tctcatgcgc ttaagtgatt catcttttcc caaaaaagcg attatgccaa
    38401 tcaagtcagg tccgccgcct tttccggtaa ttgaatgccg aagcgcctgc ataacttttc
    38461 ccgcaggcat ttcaatggtt accaacgtgg attccaatga ttctttaata ttggaagcgg
    38521 taaataaatc cagtgaatgc caggtattca tcaaggattg aattgccttc tttgtatcgg
    38581 cattccattt cctggcaaca acgttttctt cataatgtga tggagcttcc cataaatacc
    38641 gggcttcagt aaaaagttca tgcagaaaag aaacccgatc tttcataagt tcaaccgctt
    38701 cggaaacttt ttcatcagaa tattttattt ggttttcttc cagagaaatt ttcaaactgc
    38761 ttatcatctt ttctttaggc aatttatgaa catactgctg gttaaaccag attgcttttt
    38821 caaaatcaaa tttagaacct cctttttgaa ttctttccac ggaaaaatct tcacaaagct
    38881 ctgctaatgc ataaatttct ttctcggttc caggattcca tcccaaaaaa gcaaggaaat
    38941 ttatgagtgc ttccggaagg aagccttttt ccctgaatcc ttcggaaacc tcgccggtgt
    39001 tggaatcttt ccaggagatt ggaaagatag gaaatccttg tttgtcagca tcccttttac
    39061 tgagtttgcc attgccgtcg ggtttcagga gtaagggtaa atgagcaaac tttggcatag
    39121 tagatttcca gtcaaaaccc tggtaaagca aaacatgaat gggagttgat gaaagccatt
    39181 cttcgccccg gataacatgc gatattttca tcaaatgatc gtctactaca ttcgctaaat
    39241 gataggtcgg catgccatcg cttttgagaa gaactttgtc atctatattg gatgaattaa
    39301 attccacgtt accacgaatc aggtcggtaa atgaaatact ggtattctca ggtactttca
    39361 atctgatcac ccgctccgat tctgatgcca tcaaattcct ggtttcttct ttgtctagtg
    39421 ataaggaatt cttcatttca ctacgggtga gatagttata taactgagta tcactttttg
    39481 cttcctttaa tcttgcccgc attgcctcaa gttcttgggc tgtatcaaaa gcgtagtagg
    39541 cattaccgct ttcaataagt tgatcagcga acttttggta aatatttttt ctctgagatt
    39601 gccggtaagg accgaagtct ccgccttcgc ggataccttc atcgggccgg atgcctaacc
    39661 attctaggga attcatgata tactcgagtg cgccatcaac aaatctgttt tgatcggtat
    39721 cttcaatacg caaaataaat ttgcccttat ttttctttgc aaatagaaaa ttatagagcg
    39781 ccgtgcgaac gcctccaata tggagaggtc cggtgggtga aggtgcaaat ctaactctga
    39841 ctgacataaa atatttaact tacaaaacta aagtattgga atcaatcatt cagtaaaaag
    39901 agaataaaag gccttacgtg atccttttac ttttcgatga aaatgtgtgg atgtaaaata
    39961 ataattgaat aggaatgctg ccatagaaat aacggcatat aaattattgt tgtattgaaa
    40021 aagaaacaat aaacacaata acatggtaac tacagaccaa aaagagacaa ataatgccgt
    40081 ggagaatagc aaattatagg aagtgaaaat tatagatcct tttgaagttt tttcaatgct
    40141 tccttttata aatggaataa aattatcggg gcgatctaac ttttcagaaa ttctgaaaga
    40201 atctgattca atgtgtccga taaatttctt ctgatgcccg gattttcttt caaccggatg
    40261 aagggtattt tctgagagga tctgaattac tttgtcagat gtgaaaggca aaaccagggt
    40321 ttcatgataa tagggtagaa tcctcattta atggcaatac aggaaatttc cacatttaca
    40381 tttttgggta atgcagaaac ttccacggtt tcccgtgcgg ggggaattgc tccaaagtag
    40441 cttccatagg cttcattgat agcctgaaaa ttacccatgt ccttcacaaa aatggaacac
    40501 ttcactacat tttcaaagtt aagacctgct tcttcaagaa tgtttccaag gttcttcatt
    40561 acctggtgag tctcctcttt aatttcgtta gttatcatgg caccattttc cctgtcaata
    40621 gcaacctggc ctgagacgaa caaaatatca ttggcccaga tggcctggct ataaggaccg
    40681 ataggcgcag gggctttttt agactcaaca acgtttttca ttattgttta agaaggttat
    40741 gttaaatttg aacgaatttg gattccaaat tacaaattgt taaagacaaa atatctatga
    40801 aaatccttgt agttgggaat attgatcagt ttgaagagct aaagcttaaa tttggagccg
    40861 atcacgttta ctcctctctt gaagattcag acaatccgga aggatatgat ccggaggttg
    40921 tatttgattt tgtggcggaa gagattcccg aatcgcttga aaattatgag aagattgagt
    40981 gtcctgtttt cttttccata gtaaaaacaa gccttgctca tcaaaatatt tattatccgg
    41041 taaaagacca atggtttgga tttaatggat taccaacttt tataaacagg gaactcctgg
    41101 aagtttccat ttttaattcc aagggagaag gatatcttag caagattgca catggcttaa
    41161 aaactgatta tgtgcttgtg gaagatcggg taggtatggt ttcaccaagg gttgtgtgca
    41221 tgataataaa tgaagctttt tataccaatg cggagggaac agcttctgaa aaggacattg
    41281 atcttgccat gaaactgggc acgaattacc cattgggtcc atttgaatgg gcagaaaaaa
    41341 ttggtattga aaatgtttat gaagttttgg aagcagtata tgaagacacc ggagatgaac
    41401 gttataaaat caatccacaa ttaaagaaga aatacctgaa agaaattacc aggtaacgat
    41461 ggattaaaaa cctattctac agtgactgac ttggccagat ttctgggttg atccacattg
    41521 caacctctca aaacggcgat atggtaggca aggagttgaa gaggtattac agacaaaatc
    41581 ggagttaatg cttcatgagt ttttggaatc tcaattacaa aatctgccat atccctgaca
    41641 atagtgtcgc cttccgttac tatggctatt atccggcctt ttctggcttt aacttcctgt
    41701 atattggaaa cgattttttc gtaagagcta tcctgggtag caataaacac aacgggcatt
    41761 tctttatcaa tcaaggcaat gggtccatgc ttcatctcag ctgccggata cccttcagca
    41821 tggatgtagg aaatttcttt taatttcaga gctccttcta atgcaacagg aaaattaaag
    41881 ccgcgcccca aatagagaaa atttcttgat tctgaaaagg tttcagcaat tgttctgatc
    41941 ctctcattaa ccgctaatgc ccgttcaagt ttagaaggca gattctccat ttcgaccagc
    42001 atattacgga aatctctttc tttgatggtt cctttttggt gagcaattct gagtccgatc
    42061 ataaaaagaa ctatcagttg cgcagtaaag gcttttgtac tcgccacacc gatttccgga
    42121 cctgcatggg tataagcacc ctcgtgtgtt attcttgaga tcgatgaacc cacaacattg
    42181 cagactccaa aaattattgc acctttaatc tttgccattt ctattgcagc aatggtatcg
    42241 gcggtttcac cagactgaga aattgcaaag acgacatcgc cttctttgat aacaggattc
    42301 ctgtatctga attcagaggc atattcaacc tccaccggaa ttcgacataa ttcttcaaaa
    42361 aaatattccg ctaccaatcc cgcatgccag gaggtgccgc atgccacaat tacaatccgc
    42421 tctgcgttct caagtttgtt ggcatattct cttatacctc cgagcatcag gtaacccttt
    42481 tcagaattta atcgtcccct catacaatcc aaaactgatt ttggttgttc aaaaatttcc
    42541 tttaacatga aatgctcaaa cccgcctttc tcaattgatt ccagttccag atccagtttc
    42601 tcaatgtagg gagtcatctg tatgtcctgg gtatttttta ttctaaactc agcaccttca
    42661 aaaaccacaa tttcctggtc attaaggtag atgacatctt ttgtatattc gataataggt
    42721 gtagcatctg atgccaggaa aagttcaccc ttaccaaccc caataaccag cgggcttcct
    42781 ttgcgtgcgg caaccagcat ttcaggatga tcggaagaca tcactactat tgcataggcg
    42841 cctacaattt tgttcagtgc aagtctcact gcttcctcaa gtgaacattt ttcgttttca
    42901 taaatatctt ctatgaaatg aattaacact tcggtgtcgg tctcactttg aaaaacatgt
    42961 ccttttctct gaagctcctg cttaatggca gtataatttt caataatgcc attatgtatt
    43021 attgctaatt tgcggttgga actataatgg gggtgagcat tggtatcgtt tggttcacca
    43081 tgagttgccc agcgggtgtg ccccattccc aggtgacttt caagatcttc tttcttcaga
    43141 atgttctcca gatcgattac tcttcctttc tttttgaaaa ccttcagatc ggaattcatc
    43201 acggctacgc ctgcgctgtc ataacctcta tactcaagcc tcttaagacc tttaatcaga
    43261 agaggatata cttccctata tcctgaataa gcgacgattc cacacatgcc taatcaaatt
    43321 tagaataata aagccgaagc ttgacatcgt tttttctcac gacaaactgg tcaacggaat
    43381 taacgcgagg gtaataaatt ctttctggcc tcgtgccatc ccgggccggt acgaggggaa
    43441 gaacaacaag agaactgtcg ctaagttttt cagagaggat attattcaca aagaatgtaa
    43501 cttgctcgcc gtacatgtaa ttaatgctat catacccaaa aaggaacggg tcattggcaa
    43561 gtgtagggag gaaaagtatg ttggggcttt cgatctgaat gcttcgaggt atcgaatcca
    43621 agtcggaaat aacaaaacgg ttacccgcat taacctgata atactggata ccatcaggag
    43681 gattcaagga ggttactgtc ttatctacat taattatcaa atccgctctg tttattatca
    43741 catttgcagc tgtgtctttg aaattataca agcttgtaag atcaagttta agaaataacc
    43801 cgagcgcggg attatgataa gcaaattcgt tttgagggtc aaagtcagaa tacgaattac
    43861 ccagattttg aaatggggtt cctgtaaaat ctgagcgcac atttgaaaac cctcgtctta
    43921 cccgaaattg atattcttca tcaactttct ctccatcgtc atcaaaatag tgataaaaaa
    43981 gcgacattct tgtcgaatct gctactggat taaatcctaa aacaccaccg tttatatttg
    44041 ttgaataaac ttttagtccg gatagatatt ccctgaaata agcttgattt cggaaagtgc
    44101 tatcctgatc gtaaatcata ttattgagca gttgttcacc gtaaccgtcg tttaacttaa
    44161 aatagaatgt attaaggtca ccggaattat atgatcgatt tctggcgcct agcaatccgg
    44221 aactgggtcc aagaactaaa ggtgtttcaa tagagtcttt ggtaaaataa acagatgttt
    44281 caatgtttcc ctggaatgtt cttgcttcaa gtgacataac cgcattgggc tgagggccat
    44341 acgcgtagtt aacgtccata aataatacaa gggaatcaaa tatggaattt tcgggaattt
    44401 caaaatcaat ctcggtaatt gccaattcca tataagcggt agcagaaatt actccgaagt
    44461 tggcgtcctg gtacctccca aataataaag aacttgagtt ggttgagttt accgaatcca
    44521 taaatgcaac tttaggattg agaggaagat caacaaatgt tacttcaaaa gaatttggca
    44581 gggattcagt tatccctatt tcccctggat cttcacatga caaaaagaat gcagccacca
    44641 aagccggcag cagtactttc ctacccaaca agctcattat ataagttgaa ataagaatct
    44701 gtaaagctat cccctttttc aaatgtatcc acctttttgg ctctggccat tttctcaaat
    44761 aaaccattca ggctcttgtt tacagactga tcagaagtga tcacggcatc agagtactcc
    44821 attcctaatt taacaaagcc ttcaaaatct cctgtcttaa gattagagag catttgatct
    44881 tcaatatcaa tcatcttcac tttttcgata aggttgctct tgaatttgtg agagaactta
    44941 ttattgtata ccgtgaaaac tgtttttgtt tctttgaata aaggatcatt cttataggtg
    45001 gttttcaggt ataatggaat cagacttgtc atccaatctg tgcaatgaac aatatccggt
    45061 ccccagccca atttcctgac cgtctcaata acccctttgc agaaaaatat cgctctctcg
    45121 tcattatcat cataaaattt attttcctta tcatgaaaaa cagattttcg ctgaaaatag
    45181 tcttcgttgt caataaaata aacctgtagc ttggcattgg gtatagatgc aactttaata
    45241 atcaaaggct tttcttcatc accaacggaa atattgattc ctgataatct tacaacttca
    45301 tgaagccggt tttttcgctc attaattagt ccgaaacgcg gaaccaaaat ccgtatttcc
    45361 atacctcttt cctgcattgc ctggggcagt tttcttacaa aatcggcaac ttctgttgtc
    45421 tgcaaaaaag gattgatttc acttgccaca tatagaattc ggaatttagc catattagtt
    45481 gagtttagat tggtcgcgaa aacagaccac aaaggtacta aattttgaag gtaattcaat
    45541 acaatcttta gtcaaaccag taattttggc cgcaattaac ggttttgatg atttatatga
    45601 aggtattctc tacaatcaaa agtttgcaga accaccttaa atctgttcgc gcacaaaaaa
    45661 gatccattgg tctggttcca actatgggct ccctgcataa gggtcatcac gcacttattg
    45721 cgcaatccag aaaagagaat gatgtaacag tttgtagcat ttttgtaaat cgcaaacagt
    45781 tcaataacaa aaaagatctt caaaaatacc cgcgaaacct ggccaatgat ttacaattcc
    45841 tgaagtcatg gggatgtgac acagtatttg ctccctctga aaaagaaatt taccccaatt
    45901 cagaagctga gttgacgatc aattttggaa ctgttgataa aattctggag ggtaaattca
    45961 ggccaggaca tttttcggga gtcggaatta ttgtttccaa attatttaat atagttacac
    46021 cggatagggc ttactttggg ctaaaagata ttcagcagtg ctttattatt cgtcacctgg
    46081 ttgatgagtt atcttttgga gtaaaactaa gatttttgaa aacagtgaga gaagaaaatg
    46141 gactggctgt ttcttcaaga aataaaagat taagtaagaa ggagtttgat gtggcatccg
    46201 atatattcag aatattgaat cttgcgaaga aagagattat ggtgacagga gatataaaag
    46261 gtggcatttc aaattcaaag aaaattctaa aaggaatgaa accaatagaa ctggaataca
    46321 tggaaccggt gaacatgaaa gatttctcca tagtaacatc tgaaattcaa aaagattcac
    46381 gggtagcaat ttgtgtcgcc gcgtatgtgg gagaagtgcg actgatcgac aatgttatat
    46441 ttaaactgac ccatgaataa gactcaaatt aattcatgga ttcaatatct tatcattatt
    46501 gtaattactg tggtattagt atggtttgca gtatcaaata ttgaaacagg caaggaagag
    46561 tctaaattgg attttctgtt ggaggtctgg aatggagttg ataaaggatt gctggttctc
    46621 tccggagttg ttgctttgtt gagccacgta gtaagggcag aaagatggaa actgtcgttt
    46681 caacctatgg gtttgcacat ttcattaaag catagctttc tctctgttat ggttggatat
    46741 tttatcaatc tgctcattcc aaggggcggg gaaatttcaa gatgcgttaa tcttaaccga
    46801 ttagactcca ttccagttga cagatcattc ggaactgtat tggccgagcg ggccgtagat
    46861 attgttttcc tggttttctt tgtgctgcta ggttttctat tggagtttag taaaatcaac
    46921 cttttccttt caagtctgca atatgataag agtaaagact ggtcgttaat tattaagtat
    46981 gtattgcttt tggtatttgc tctgatcgct ggaataatat ttctcaggtt gttgcttaag
    47041 tcgggaaaac caagggtatt gcggacatat ataaaattta aaactattat gaagggaata
    47101 aaagaggggt tgatggttgt tatcagatta aaacaaaagt ctctattcct tttttatacc
    47161 gtaataatct ggttgctgta tctgttcatg agtgtttttg ttttgatggc ctttgaagaa
    47221 acgcaacatc ttggatttgt ggcggccctg gcaatttttt caattggagg aatagcgatg
    47281 tcattaccct tgccaggcgg aacgggcagt taccatattt tggtacctgc cggtctggtt
    47341 gctctttatt caataccgtt ggataaggct acggcgttta ctattatttt tcatggctgg
    47401 caaaccatca taattattat ttttggtgca gggtcgctct tttacagtta ttatttacac
    47461 aataaaaaaa ggagatggaa cccctaacca atgacgaatt gtcagtaatt tcaattggtc
    47521 taaatattgt taatacaaac agacaaaata cgataactga attatgggat taattgtaat
    47581 tatgattttt ttcgggatcc tgagcttcgt ggtgagctcc aggttaaaaa gcaaattcag
    47641 gaagtactct gaaacgccaa tcaggagtaa cttatcagga aaagaaatag cagaaaaaat
    47701 gcttgcagat cacggcatat atgatgtaaa ggttatctca acaccaggtc atttgtccga
    47761 ccattataat ccggccacta aaacagttaa tttaagtgaa gcggtttata atggcaaaaa
    47821 tgcagctgca gcatctgtgg cctcacatga atgcgggcat gccgttcagc atgcgcgcgc
    47881 ttatactatg cttgaattca gatcagccct ggtccctatg cagaatatta gcgcaaaagt
    47941 gatcaatgta atctttatga taagcttgtt cggcggattc ttcctcttca atgcgtttcc
    48001 cacaaatctg gttctgactg taataatcgg agcctatact attatgacac ttttttcact
    48061 ggttactctt cccgttgagt ttgatgccag caaaagggca ttagcctggg taactgacaa
    48121 gggtatcgtt tcatcatccg agcatgagat ggccaaagat gctctgaaat gggctgcttt
    48181 aacttatgta gtagcagcgg tgggttcgct ggtaacatta ctttactggg tatttgtatt
    48241 gctgggaaac agagactaaa attgggcttg aatatccctt tcttagtcta aaatgaattt
    48301 tcaattcacc gaagagcaat tagcggtaaa agaggctgcc cgggaattcg ctcaatccga
    48361 acttcttcca ggtgtcatag aaagagatga aaatcagcaa tttcctaaag aacaggtaaa
    48421 gaaattgggg gagctgggct ttctctccat gatggtggac ccgaaatacg gcggtggggg
    48481 tatggataca gtctcctatg tattggcaat ggaggaaata tcaaaggtgg atgcctcctg
    48541 ctcagtaata atgtcggtga ataattcatt ggtctgctgg ggaattgaaa agtttggttc
    48601 aaaggagcag aaggaaaagt acctcacaaa gctggcaagt ggagcggtta tcggcgcatt
    48661 ttgcctgtca gagcctgaag cgggctcaga tgccactaag caaaacacca ccgccattga
    48721 tatgggagat cattacctgt taaatggaac aaaaaattgg attacaaatg gcaattcagc
    48781 aggaatatac ctggtgattg cccaaacaaa tcgtgagaaa cggcacaggg gcattaatgc
    48841 tttcatcctg gaaaagggaa tggaagggtt tattgtgggg aagaaagaaa ataagctggg
    48901 aatccgtagc tcagacacac attctttaat gttcaccgat gtaagaatac ccagggagaa
    48961 tagaattgga gaggatgggt ttggtttcaa tttcgctatg aagacgctaa atgggggcag
    49021 aattggaatc gcctctcagg cgttgggaat cgcctcgggt gcctatgaac ttgcattgaa
    49081 atattctcat gagcgaaaag catttggtaa ggagatattt aaccatcagg gcatcaagtt
    49141 caagttggcg gatatggcaa ccaaaataga agctgccagg cttttatgcc ttcaagctgc
    49201 cgttttgaaa gaccagggta aagattttgt caaggaagcg gccatggcga agctttatgg
    49261 ctcccaaaca gcaatggaag ttacggtgga agcagttcaa atccatggtg gctacggcta
    49321 tgtaaaagaa ttccatgtag aaagattaat gagggatgca aagattaccc agatctatga
    49381 agggacgtca gaaatccaga aaattgtaat ttcccgggaa ttattgaagt aaaacttcaa
    49441 tcagattctt tcaattatcc aataaattta aataattggt ctttttgata attgtattat
    49501 tttattagat ttgtccaacg tggacctctc ccccgtactt tttaagcagg ttaatatcaa
    49561 aatcggataa aaatggaaga ttataataaa attattgaat cgttaggggt tcggtttaac
    49621 aaggctggga atattaatat tttgcaaccc gctaccgtaa ccaattacta cgatgttgag
    49681 aacatgattc tcgtgttaaa tcgtggtgaa atacgtttcg gagaggataa taagggagta
    49741 ttggaaggtg aggtcttgtt tatcccgggg ggtcgtcaaa tacctatcac ctacggaacc
    49801 ggcaaggcac atgtagtaaa caatgaagag ttcacagcaa agagaaaaaa ctattttaga
    49861 agttatatca acattgaatc ggatgaccag aaatatgatg attttaccta tgtttcattt
    49921 gatgccaagg tatttgattc ggtcagtttc tttacatctc tggatgttcc gccatttgta
    49981 attaatgatc ccaatataaa tgctttaata aacagcattt tgaaagaaaa gggatcagac
    50041 ctggagggga aagacagggt gattaaactt cagacagaat atttggtaat tgaaatagta
    50101 agatacataa ttaagaacag actatttgta gagcaattgg caaccaacag cacatatttt
    50161 aaagacccgc gccttattga tatctttggc tacatcaagg aaaatctcgg aggtgatttg
    50221 tccaacaaaa cactggcttc agttgccaat gtttcagaag attatgttgg ccagtatttt
    50281 aaaatgctta ccggaattaa cccgcaagat tacattgagt atcaaaggat ggaaagagcg
    50341 gtggatttgt taagaactac caaaaaaagc atcagggaaa tcggcaagga agttggctat
    50401 aaagacacag cttatttttg ccgtcgattc aaaatgatgt ttggaatccc agcaggcaaa
    50461 atgcgcagac gagaatcttt aatgaacctt tagcgattca ttatactcac ttactgtaag
    50521 tgaaatatta atcaacaggt ccttggaatt ttcaattcca tttccaacca caattatatc
    50581 agctccggct tttaatgcat gcattgcatc aatttccgtg cggattcccc cgccaataat
    50641 caacggtata tttatggatt tgcttacttt ggaaatcata tctggtggaa tgggacactc
    50701 agaaccactg ccggcatcca tataaatcat ttgcattccg agcatctcgc ctgccatggc
    50761 cgtagcgcat gcaatttcag ttttgtcatt gggaataggt atggtattac tcacatacgc
    50821 tgccgaagat tttgcatgat catttaccag catatacccg gtgggaagaa tttccagtga
    50881 tgtcttccgg agaaagggag cggctatcac atgctggccg ataagatatt cagcgtttct
    50941 ccctgaaatt aatgacagga ataggatagc atctgcatga gaatctatgt gattgtagtt
    51001 tcccggaaaa agtatgacgg ggatgtggga attatccttt agctttctaa ctactaaccc
    51061 gatattgttc ctggatataa aactgccacc aacaaagaag tagtctacat tgttctcaat
    51121 ggctatatta attagtccct gcagatcttt aacctcttcg actttgtcag gatcaattaa
    51181 gacggcaaaa cctttaattc ctttttgttt tctatcaatt atctggctga ttatcatctt
    51241 ctacttgctt tctttcgcta aggtattcaa tcaatttttc tttagccaga tttatcaaaa
    51301 ggagcgtggc tgcttcagcg acatattttg ccacgtgacc aaaagcatta cctggctttt
    51361 ggtggtcctc tggtttttgg ttgtctgttt ggtctgaatc tgacgtaagc cctttcacaa
    51421 ataaataggt aacgtaggct aatcctccca caattaatcc tgtaataata cctctttcga
    51481 ttttggaagt catttcagaa agatcctccg caaagttctc cttgtatcgc tcagagtttt
    51541 caagcaatgc cgccttttca attttcggat tcttcacttt catcttttgt cgtactaagg
    51601 taactctcta tatacttcct gattcttatt ttcaaattca aaagcttctg caaaagagcc
    51661 aaaagaagga tcagaaataa ataaaagcct gacacaatgc caaatcccca gtatctgctc
    51721 tccagaatgt cattcagata atcacttagg aagaaactca ggaaaatggt gaagaataaa
    51781 atcaatatag cgaagcaggc aagaatgaga agtttggctc cggtttcagc cgcttcttcg
    51841 cgtaactcca gctttaaaag ttcaatccgg ttttcgacaa aaccaatcag gttgttaaca
    51901 attctgtcta attttaaaaa gctgaaaatt ccttttttct cttccaaatc catcaaaatt
    51961 acataaaaag cgtggtaatt ttattcagac aagaaaagaa taaggaatgt gcaggcgcgc
    52021 ttacttttcc ttcttattcg gatctttctg cagtttcttg tgtttcatga tttccgcttc
    52081 aatataaaac acggcattct cggcaatgtt ttttatatga tcaccgacgc gttcaagttt
    52141 gcggattacg gaaaaaagaa ataataaaga gcgagttgca tccgggtcct ttttaataaa
    52201 ctttctgata gtttgggatg ccatgctgtt aagggaatta agttcagcat ctttcttgta
    52261 cacttttctt gccaaactgg tatcttcatc gttgagcgcg gtgctgatat cggttagcat
    52321 tgaaagagtt atatggaaca ttttaccaac ttctgtagca ttaattgcat cggatgaaat
    52381 tgcttcatcc atttccaaca catagtccgc gataccatcc gcgtaatctc ctatccgttc
    52441 taaatctgaa ttaattttga gcatggaaat tacaaatctt aaatcggtag ccaccggctg
    52501 aaataaggca agaatatttt cacagtcgag atcaatgctc agctccattg cattcatcct
    52561 tttttcattt cgtgtaattt cctttgcaag cttgaaatct gaattgatga acgcttcctc
    52621 agacttcttc aactgaaatt ttgccaggtc catcatttct aggatgcttt gcttaagctt
    52681 tgaaagttct ttttcgaggt gtgtcatcat tccatgaatt tttatccgaa gcggccgctg
    52741 atgtaattct cagtgcgctc atgttttgga tttgtaaata tctgtttagt ttttccgtgt
    52801 tctacaagtt ctcctaaata gaagaaagct gtgttatcac tgaccctgcc tgcttgttgc
    52861 atgttatggg taacaataac aatggtgtat ttttctttaa gttgaaaaat cagttcttca
    52921 attcttgctg tcgaaatggg gtcaagagat gatgttggtt catccatcaa aataacggat
    52981 ggttcaacgg ctaatgttct tgcaatgcat actcgctgct gttgtccgcc ggaaagtgcc
    53041 agcgccgatt tcttgaggtt atcctttacc tctccccaca aatcactttg tttaagtgct
    53101 gtttcaactt tttctgaaat aaatttttta ttggtgatgc cctgaatttt taagccgtag
    53161 gcaacatttt caaaaattga tttcgggaat ggatttggtt tttgaaaaac catacccaca
    53221 tcttttctca attgttcaac ttcaattttc tttccataga ttttaaaacc atccagtctg
    53281 atttcccctt ccattctgaa ttcatctatg taatcattca ttctgttaaa taacctgaga
    53341 aaggtggatt ttccgcagcc tgaaggccca atgagtgcag ttacatggtt ggcagtcatt
    53401 cccatactta cgttcttcag ggcatgaaac tgaccatacc aaacatccac gtgactagct
    53461 tccaatttga aacttctgga ctcgtgatca tttttcttat atccttcttc ctttaattcc
    53521 atcaatgcaa tcatattctg gttgaagtta ccatttcatt tttttctgcc atctattgcg
    53581 aaaatacaca gctataccat tcatcaccaa agtaataagc agcaatatta taatcgcggc
    53641 tgcagcattg gtaatgaatc cctgttgcgg gcggctaacc cagttaaaga tctgaatagg
    53701 gagcacggag aactcgtcca tgggagattt cggtgcaaaa ggaacataag ccaacgcacc
    53761 tataacaatt aaaggggccg tttccccaat agcccgtgaa agtgccagaa ttactccggt
    53821 gagaattccg ccaaaagatg ccggcagaac ctgattccga atggtttgcc actttgtggc
    53881 acctaaagca aatgatgcct ctttaatgga gccgggcacc gcttttatgg attctcttgt
    53941 agcaacaatt acaattggaa ggatcagcaa tgataacgtc aggcttcctg caagtacgct
    54001 agggccgagg ttcattaccc tgacaaagat ttccagtccc aatagcccgt aaattatgga
    54061 aggaacgcct gctaaattgg aaatattaat ttccaataaa tttgccaggt gacttttctt
    54121 tgcatactcc tccaaataga taccggctgc gactccaagg ggaaatgaaa taatagtggt
    54181 aaaaaccagt acccacgcgg ttcccatcat ggcggtccat attccagcac tttccggtct
    54241 gcgcgatggt agcgcggtaa taaaatccca actcagtctc cccaacccat caatcaaaat
    54301 gtctcctata aatattacca gaagtaccaa gccaatgaat gtgcaggcaa tcccccaaac
    54361 ctggaaagct ttatcagcaa ttctgttttt gcgcctgtta ttcatacttc tgtctatact
    54421 tcttgcggag gaaaaaactt atattattca acaaaaaagt tagtgcaaac aatgtaattc
    54481 ctgcagcgaa aattgttctg tattcaagtg ttccatgttg cacatcaccc aggctgacct
    54541 gaacaatata cgctgtaata gtctcaatgg gtacagtggg atcaaatgta agtctgggct
    54601 gctgaccggc agcgattgct acaatcatag tttctcccac agctcttgaa atggcaagaa
    54661 taatggagac aacaattccg gatgatgctg ccggaacagt taccagcatc gctgtttgca
    54721 gccgcgttga tcccatgcca taggaggctt ctcttaatga ttttggtaca gcgaataatg
    54781 catcttcact caatgaagaa ataaatggga taatcatgat tcccattaca attcctgccg
    54841 agagggcatt aaacccggcc aggtctggat atatctgttg taaaaaaggt gtaaccacca
    54901 tcaatgcaaa aaatccataa accacagtag gaattgctgc gagtaattca agggcgggct
    54961 tgatggtttt tctaaacccc ttgggtgcat actcatttaa gtaaatcgcg atgctgaggc
    55021 ctataggcaa tgcgacgaaa attgcaatga aggttgttaa taatgtacca gtgagtaatg
    55081 gaaggatacc gaaatgtttt tgttcaaata gtggcgtcca ttcggtgtct gtaaaaaacg
    55141 atattaatga aacttcacta aaaaagttaa cagcttcaac agttaagacc aggataattc
    55201 caatggtggt gaggatagta ataaaagcgc ttaataagag acttttttca atcagatatt
    55261 ccttaatctt tctcatacct gaaaacagaa aaccaagtat cttcttagac gacccggctt
    55321 gcaattggag tgattatcct gagagttacc ttttagtaaa atcattaaat tttgaaatct
    55381 cagcactgta aaggtcgtca ggcatgggta tatatcctac ttcagctgct aagagtgaag
    55441 cattttcaag ataaaaatct acgaaggaaa tcacctccat cctctcaatt gctgtgctgt
    55501 ttacataaat aaagaggggc ctggatagcg gtgagtatga tccgcttctc actgtttcca
    55561 gagaaggaag aatagggcca tgaccaccat caacagccac aaccttcagt ttatctttat
    55621 tctcctcata ataggcgagt ccgaaaaatc ccagcccaaa cttatcgccc gcgattccct
    55681 gcaccaaaac attatcatcc tcgctggctg taaaatcgcc tctgctggag ccagttaccc
    55741 caacaactgc ttcggtaaag taatcgtaag taccggaagc aaccccgggg ccgaataaat
    55801 gaacctcttc gtcaggccaa tcgggtctga tttgattcca cttcataata gttccctggg
    55861 cagcaggttc ccagattttt tttaattctt caaccgtgaa gttatcaacc caattgtttt
    55921 ccgggtttac aaccactgta agaccatcgt aagcaacaga aagttcaaca taactaattc
    55981 cattcttttc acaggctgct atttcttttt ctttgatagg tcttgatgca tctgaaatat
    56041 ccgtttcacc ccgggcaaat ttttgaaatc ctccgcctgt gccggatacg ccaattgtta
    56101 ctctaacacg tggggatatt gccctgaatt cttcagccac ggcctctgtg attggataga
    56161 ctgttgagga gccgtctatg gagattgacc cggaaagctc agtagcatca gcttttgctg
    56221 attccccgga attgctgccg catgcgtata aaagcaatgc cgaggtaaga attgtgtgaa
    56281 ttgtaatgcc tgtcttcatt ggtcaaaatt tggtttataa tatgaactga tgcttatcca
    56341 attgttaaca ttcagttaag ttgaaatgat ttcctgctta aatccggcca aaaataacgc
    56401 aatccggctt agaaattcaa ttgggcctga attcgcagca aactgccctc ctgtaaatta
    56461 tcctgattaa taaaatcttc atagcgtctt cgtgaatgag tatacataag caccaattca
    56521 aactgcttta aaggctgcca ttcaatccct aactcaaatt cacttacctc ataactcctg
    56581 gcatctcgct catgtttctt ccctccatta tagtattgaa ccctggtaaa tggaattatg
    56641 atctggtttt ttattttcgt taaataactt gccgtaagat aaccgcccct cagcgatttt
    56701 acttccaccg aatccgtagc attattaaat tccgggcctt ctccgatatt atattccgcc
    56761 tgaattccaa aaggcttagg atagagaata aaagtgcctg ctatcctttg atccgggtaa
    56821 cttaagtcgg ttttgtattt aactcctgca gacaaatttg attgtggaat tacgtaattg
    56881 ccggtgtatc cctgaacgct tagctcgaaa acctgacttc ttacttcaaa aggataggtg
    56941 gccctggcta caaaatgcgg ctctttattt atttcaagac tgttagcagt ttgaccatta
    57001 tatattccaa ttcctaaaat tccgtagtca ccgcttccct tcacgccggt tcggacaaga
    57061 cttgatagta gtttcccctt tccagcaggt gcccaaaaaa agaaaacccc taagtctctt
    57121 tcatttgaca ccgcgctgtt taaaccatca tttctatcta agggtaacct gttttgactg
    57181 gattgtaaat tttcgaatcc aaaaggaact ttgctttgac cgattctaaa tcgaaattca
    57241 ttttctttgt cgagcccgat atcaaaataa gcatcccgaa gttgaacgaa gttaagttta
    57301 tccgaaccgg ggctgcttgc aaaatcgggt tgcacataga aataaacatt cctggtaatt
    57361 tgaccataga atattattcg cattcgcctt ataaaaaatc cgccgccttt accccaggat
    57421 ttatcacatt gttcgcaacc aagattctca ttggtttcaa acaaccgatt gtatctaacc
    57481 tgtgcatatc ccctgatgga aatattgtcg taccactcgg tttcgacagt attttgttct
    57541 ttggtttgac tgtaagcatt aaaattgctt atggccatta tcataacaat cagcattaat
    57601 ttattcatca tacatatcag gataaatttc ctgctaatta aggatacctt atgtatttga
    57661 tgcaacactt tttcataatg tcataaataa ttaatccgaa attaacaatt tgataataaa
    57721 gattttaggg tcaactcagg cttattttat taatgaacag cgcgttagac tatttccttt
    57781 cgagtaaggt ttgttaacat tttgataaca tactatttta ccctcaaaag cattacactt
    57841 gtacttttga aagtatttct aactgaaccg ctatgaaatt cggctttttg gacctacttg
    57901 aattaatagg tgcgttgggt ctgtttattt tcggaatgaa aataatgagt gaagggatcc
    57961 aaaaggctgc cggatctcaa atgcggagga tcctgagggc gatgacttcc aacaggttca
    58021 agggtataat gacaggtttt gccatcacca gcattgtgca atcttcatcg gcaacaacag
    58081 taatggtggt aagttttgtt aacgccggct tgctttcatt aatggaagcg atcggtgtta
    58141 ttatgggagc taacatcgga accacgctaa cggcatggct tatttcaatc attggtttta
    58201 aaatgaaaat tgtgtcaatc tctttaccaa tgattgcttt cggttttcct atgctatttg
    58261 caagtaaaag tactaccaaa gcctggggag aggttttgat tgggttcgcc attctcttaa
    58321 ttggtcttga tgaacttaaa aataccgtgc cggacctcag tgcgaatcct caaatgtttg
    58381 aatttctttc cagatacacg gatttaggag ttctgtctac tttgctattc atcttaattg
    58441 gtacattatt cactattgca gttcaatctt ccagtgcagc catggcctta acactggtta
    58501 tggcgaataa cggatggatt ccttttgaaa tggcagccgc catggttctg ggtgagaata
    58561 ttggtacaac gattactgca aacctagcgg cgatagtggg aaatgtgtat gccaaaagag
    58621 cggcccgtgc acacaccatt tttaacttaa ttggtgtcat ctggatgatt atcttgatta
    58681 acccttttct cagaaccatt ggtaattatc tcatttctgc tcagagtatg aatcccttta
    58741 tggatgcaac tgcaattcca attgctttat ctgtatttca tacttctttc aatattctaa
    58801 atactctttt gttggtctgg ttcacaccgc taattgcaaa agcagttgaa aaaattgttc
    58861 aatcaaaagg agatgacgaa ttccatcttg aatttattgg aaaaggaatt atcaggactc
    58921 cggacatttc aattcttgaa gccagaaaag aaattgcaaa atttggagaa atcacaacaa
    58981 aaatgtcagg gttccttcag gctttacttt tcaaaaacaa agaaaagaag agaatgaaat
    59041 ggatggataa gattcagaag tatgaggaaa tcaccgacag ggtggaggtg gaagtggcta
    59101 attatcttac aaaagttgct gaaggaaata tcagtgagga tatttctgtc aaagtaagat
    59161 cattattgag tatcaataat gatttggaaa ggattggaga tcttttctat cagatgtctc
    59221 ttgcagtgca acgtcaaaat gatagtattc atgatttgga taaaaatcaa ctggatggat
    59281 tgcaggaaat gttccggttg atagataagg cctttgaggt gatgaacaag aatttaaatt
    59341 ctaattattc tgaagtaaac attaatgaag caaaggatat tgaaaaaaga attaatgccc
    59401 agcgtgacga cctgaaacgg acacacctga aacacattga gaaaggtcac tataatgtga
    59461 tggcgggcat ggtttactca gatgtattcc aaacgcttga gagaatcggt gaccatattt
    59521 ttaatgtctc cgaagctgtg attggtgaga tatgatttta ataaacccgt tcaggaatca
    59581 ggattgcatt ttgaaagacc tttcgcattt tttcaagcgt tttctgagca tccagtcttt
    59641 cataaaacaa gcctactttt actttgaaaa tgggttgctg atattccagt accggtatta
    59701 aatctgaaaa ttctctatac gcaagggctt ttgcatcgtt agctgctgtg ctgctggttc
    59761 cattgtaaat ctggatagta taaacctgat aatatccgcg gcttcgcgta taagaagcta
    59821 tgctgtcgag caggctgtcc aaatcggcgg caatagacgc ggtaggttca acaaatgtca
    59881 gttgttcaac ttccgccagg ggatagctgc ctgtgtcagt ctgaggatcg ggatacatag
    59941 gtctgtataa ggaaatgtct tcagaatagc ccgacgactg agtttgtgac cccactttgc
    60001 tcgcgcagga accaattaag gccaacaaaa tgaaataccc aattcgtttt accagtacgt
    60061 taatcatcaa tgagaataca cttattattc agaatgtcat cttccacctt cttgtactta
    60121 acatctcttt ttattgtccc atcggtatat tgaactgaaa ccttatcgtt tctgccggcc
    60181 atcttttctg atttcattgg agcgggtttt tgaatgggct gccggctttg gccgccgcct
    60241 gagcctgata atagtgatct ggattcatcc ttccgctcgc ttaaccttgg ctgggatcgc
    60301 tgctgtcgtg cttcctgaac ctcatcaggt tgttgtacag gcaggtctgc cttagccaga
    60361 aaggtaattg tttcctcatt tacccttgcc acaaattgct tgaataagtc aaatccttcg
    60421 aacttgtaaa taagaagcgg atctttctgt tcgtaaaccg cattctgaac ggattgtttc
    60481 aaatcatcca tttcgcgtaa gtgatctttc cactgctgat cgataatggc caggcaaatg
    60541 tttttttcca tggatttgat aagctcttta tttttagtat ccacacattt aaccaggttt
    60601 gctgaaacac ctatttgttt ggttccatct gtgaacggca ccaggatatt ttcaattgtg
    60661 gctccccggg ttttgtgtat gtctttgatt atcggataag ctttctcggc aatagtcaga
    60721 ttttttcgtt gataggtatc gtaagcctgc tgatagagtt tttctgagag atccgaatca
    60781 ttagttgatg tgaactcttc ctggctaagc ttaaaatcga tggaaaaaac actcaatgaa
    60841 tttaatttca aactatcata atcattggct cctttggcat tcaatacaat attttcagaa
    60901 gcatcataaa actgattcat aatatccaat tcaagtcttt ctccatataa tgcattgcgc
    60961 ctccgtttgt aaattaactc acgttgcgaa ttcattacat catcatactc aagcaatcgt
    61021 tttctgatac caaagttatt ctcctcaact tttctttgcg ctctttcaat ggacttggta
    61081 atcatggaat gctgaatcac ttctccttcc tgcaaaccaa gcctgtccat gatccgggca
    61141 attcgatccg gcataaaaag tctcattaga ttgtcttcaa gactcacgaa aaattgagat
    61201 gatccaggat ctccctggcg gccggacctg cctctcaact ggcggtcaac ccgccgcgat
    61261 tcatgccttt ctgtaccaac gatggccaga ccgccggctg ccctggattc aggagtcaat
    61321 ttaatatctg ttccacggcc agccatgttt gttgcaattg ttaccgtgga aggcagcccg
    61381 gcctgagcta caatttcagc ttctttttga tgttgcttcg cattcaacac attatgtttg
    61441 attttccgca ggttaagcat tctgcttacc agttcagata tctccacgga ggtagttcca
    61501 accaaaacag gacgtccatt ggtagtaagg tcaacaattt catcaaccac cgcgttaaat
    61561 ttctcccgaa tggttttgta aaccttgtcc tccatatctt tcctgacaat attccggttc
    61621 gttggtataa ctactacatc aagtttatat atctgccaga attcacccgc ttccgtttct
    61681 gccgtacctg tcatcccggc aagtttatgg tacattctga agtaattctg cagagtaatt
    61741 gtcgcatagg tttgggttgc gtcttcaacc tttacatttt cttttgcctc tattgcctga
    61801 tgcaaaccgt ctgaatacct cctgccttcc aacactctgc cggtttgctc atcgacgatc
    61861 ttcacctttc caccatcaat aatatattca gtatcttttt catataaaga atatgcgcgc
    61921 aacaattgat ttacagaatg aatccgcttc gacttaatcg agtaatcttt aatcaattcg
    61981 tcttttcttg caagtctttc tttatcactt agcgaggcat cattttcaag ggatgcaatt
    62041 tcagcaccta tatccgggag aatgaagaaa cttttatctt catcatctcc tgtaatcaga
    62101 tcaattcctt tttcagttaa ctcaatttga ttgtgttttt catcaatggt gaaatacagc
    62161 ggggaatctg cttccggcat catcttggag ttatcctgaa gataaaaatt ttctgttttt
    62221 tgcaagactt gcttatttcc ggtttcactc aaatacttaa tcaagggttt gtatttaggc
    62281 aagcctctga atgctctaaa caatgacagg ccaccatctt ttgaattttc tgcaattagt
    62341 tttctggcat caatcagata ttgggagacc agttttttct gcgcttcaac cagttttgca
    62401 attcttggtt tcaaatcatg aaactcatgc tcgtcacccc tgggaatagg accggaaatt
    62461 attaatggcg ttcgagcctc atccaccagg acggaatcaa cctcatcgac cattgcatag
    62521 tgatgttttc tttgcacaag ttcccgtgga tcgcgcgcca tgttgtcgcg caggtagtcg
    62581 aaaccaaatt cattattagt tccataagtt atatccgctc ggtacgcatt tctacgatct
    62641 tcggaattag gctgatgttt atcgatgcaa tccacattta atccatgaaa ctggaaaagc
    62701 ggtcccatcc attcggaatc cctctttgcc agataatcgt taactgtaac gatgtgaact
    62761 ccacggtcgg ccaacgcatt cagaaacgat ggcaacgtgg caacaagggt tttgccctca
    62821 ccggttgcca tctcagcaat ttttccctgg tgcaaaacaa ttccaccaat tagctgcaca
    62881 tcataatgga ccatgtccca ggtaatttca ttacccccgg ccagccattt gttgtgccaa
    62941 attgctgatt cccccttaat ctcaacgtgc tgaaacttcg cggccatttg cttatcgtgc
    63001 atagttgcgg gaacctccat tttgtgattc tctttgaatc ttcgcgcggt ttctttcatt
    63061 atcgcaaatg caagcggaag gacttccaaa agaacatcct ccaggttttt atttctcttt
    63121 tcttccagtg aatcaatttg agcaaaaata tcctccttct catggatatt cagatctggg
    63181 ttttgatcaa tttgatcgtg cagagacttt agttggtcgt caatttcagc aagatgctgc
    63241 cggattcgat cacggacttc actggtttta tctcttagct gaccgtcatt caagtttacc
    63301 aacgtgtcaa attcccgcag tgtttgctca accaaaggca tcaccgattt tatatcgcgt
    63361 tgggattttg atccgaaaac tgcagcaatt cccttcgtta aaagtctaat cataccaaac
    63421 cttaattaag gatgtcaaat ttaaagtgtt tttaagtaaa tactggtaaa cttaggattt
    63481 gattgaaata gaacccttga aaaccttctc ggcaggtccg gttaatagga tatccttaaa
    63541 agagttattt cctgattgct gaaaagctac ctgcaagtta ccaccccggg ttttgatttt
    63601 caccggactc ttcaacattt tgtaactgct ggcaagcgcg gcggcagtaa caccggtgcc
    63661 acacgacaaa gtctcatttt ctactccccg ctcataagtg cgtacaaaaa tagtgtcatc
    63721 tcctttttcc tccacaaaat tcacattggt tccgctggtg ccgaaaagtc ccccgtgcct
    63781 gatttttttc ccttctcttt gtacgttata ctgattaatt tttgccacga attggacaaa
    63841 gtgtggtgaa ccggtattca taaaaaaacc atcatgcatg agaattactg aacttacatc
    63901 tctcatcttt aaactcacca gttcaccttt gacagtggct tcatgaactc cgtcgaacgc
    63961 ggagaactcc gtttcatttt tcaccatccc taatgatttt gcgaaacgaa ccgcacaacg
    64021 ggaaccattc ccgcaaagac ttttcgagct gtcaggatta aaaaactcca tgcggaaatc
    64081 gtatttatca tcttcttcaa ttaatatcaa gccatcgctt ccaatcccaa accttcgatt
    64141 gcataattta gaaatgagac ccttattttc tttgtcaaaa aaatcgtccc gattatcaat
    64201 cattacgaaa tcatttcctg ttgcctggta tttttcaaag tttagttcca tagcttgcat
    64261 ggttaatcaa cttcttcata atccacatac tctccgccct gatatccttt ttgattacgc
    64321 ttgtctttag ggtcaaagtt gatgctcaca ttgccgcctt ttggtttttg tttaaaagac
    64381 tgattttctt tgcctgaatt acccagcaaa aagcgcatga tgtaaccccc gagtttgaag
    64441 gtaaggtaca tgaaagcaaa tagtattaat aaaaacttta acatgataaa attttacctg
    64501 ctcagatata aatttctttc tgagtagatt ttcaaaaaat aatcatccat cagatcatcg
    64561 atgaaataaa tggattcacc tgtagatttc atctcaggtc ccaattcttt gtttacattg
    64621 ggaaacttat taaaagaaaa taccggttct tttatagaat atccattctt ttttggattg
    64681 aatttaaagt ctgaaagttt gtttaagccc aacataatct ttgttgcata attaacataa
    64741 ggttcatcat aagccttaca tataaaggga acggttctgg aagcccttgg gttcgcttca
    64801 ataacataaa ccttatcatt cttaattgca aactgaatat taattaatcc tactgttttc
    64861 agtgccaggg caatcttttt agtatgagcc tcaatttgtc tgataacaaa atcaccaaga
    64921 ttatatggag gaagaaccgc gtaagaatct ccggagtgaa ttccggcagg ttcaatatgc
    64981 tgcatgatgc ctattatata aacatccact ccatcacaaa ttgcatcggc ttccgcttca
    65041 attgctccat ccagaaaatg atcaagcaaa actttattac cgggaatgtt tttcaaaatt
    65101 tcaacaatat gattttccag ttccttttca ttgataacta ttttcatgct ttgtccgcca
    65161 agcacataag aaggacgaac cagcagggga aatccaattt tctttgaaag ttcaatggca
    65221 ttttccgcat cttcggccac cccgaattcc ggatagggaa tatcattttt ttgcagcaat
    65281 gtactgaagc tgccccggtc ttcagcaaga tccaggtttt caaaagaagt acccaaaatt
    65341 ttaattccaa aacgctctaa cttctccgcc agttttaacg ccgtctggcc gccaagctgc
    65401 acaataactc cctccggttt ttcatgttgg ataatatcgt ataaatgctc ccagaaaacc
    65461 ggctcgaagt aaagtttatc ggaaacatca aaatcagtag aaacagtttc aggattacaa
    65521 ttaatcatga tagtttcata gccgcactct ttggctgcca atactccatg cacgcaggag
    65581 taatcaaatt caattccctg tccaatccgg ttaggtccgg aacccaacac gatgaccttt
    65641 ttcttcttcg atggaattga ttcattttcg ctgtcaaacg aagaataata atacggtgtt
    65701 agggcttcaa attctgctga acatgtatca acaagtttat acacacgatt tattcccagg
    65761 tctcttctct ttttatgaac ttcactttca aggcaccgga gcaggtgtcc caattgccgg
    65821 tcagcatacc ctttttcctt tgcaatgcga aggagttgct caggaattgt atccagggaa
    65881 tgtttctcaa tttctttttc tagaataagc agttcttcaa tttgatagag aaaccatttg
    65941 tcaatcctgg tcagctcctg aatggttttc ataggaatcc caagtttgaa ggcatcatag
    66001 atatgaaaca agcggttcca tgaaggattt ttcagactct tcaggatggc aatctgatct
    66061 gtcaattctt taccatccgc gccaaggccg tttcgtcgaa tctccaatga ttgacacgcc
    66121 ttctgtaatg cttcctggaa attccttcct attgccataa cttcgcctac tgatttcatc
    66181 tgtaagccaa gccggcgatc cgaaccatga aacttgtcaa agttccatct aggtattttt
    66241 actataacat aatcaactgc cggttcaaaa aaagcggaag tagtttttgt gatctggttg
    66301 ggaagctcat caagattata gccaatggca agctttgccg cgatcttagc gatgggatag
    66361 ccggtggctt ttgatgcaag agcggatgat cgtgaaacac gcggattaat ttcaattcca
    66421 atgatatcat cattctcagg attaacagca aactgaacat tacacccacc agaaaatttc
    66481 ccgattccgt tcatcatctt aatggctaaa ttgcgcatct tttggtatac cgtatccgga
    66541 agcgtcattg ctggtgcaac tgtgatggag tctcctgtat gaatgcccat cggatcaaag
    66601 ttttcaatcg aacatatgat gatcacatta ccggctccat cccttagcag ttcgagctca
    66661 aattccttcc agcccataat gctttgttca ataagtacct catgaattgg cgaagagtgc
    66721 agtccatgtg acaacgcttt ttcaaattct tccggacttt cgacaaatcc accccccgtt
    66781 cctccgaggg tataggatgg tctgatgaca agggggaaac caatttcctg ggcgatttcc
    66841 ttcccctcca ggaatgatgt agcggtttca cctctgcaaa catttactcc tagttcattc
    66901 atcttgagac gaaatttttc cctgtcttct gtggtttcaa tggcagcgat atcgacacca
    66961 atcagctgca cattgaaaat gtcccagatt cccgcgtttt gacattctat cgcaaggttg
    67021 agtgccgttt gcccacccat tgtcggcaaa acagcatcta tctcatgctt tttcaggatt
    67081 tctacaattg actttttagt tagcggtaac aaataaatat tattcgccgt caccttgtca
    67141 gtcatgatag tagcgggatt attgtttatt aaggtgactt caatgccttc ttctctcaat
    67201 gaacgggcag cctgtgaccc ggcatagtca aattcgcaag cttgcccaat aataataggt
    67261 ccgcttccta tgatcagaac ggattttata ctttcatctt tcggcatctg gttagttggg
    67321 aggtgtttag cttagcacaa attggcacaa agttagggca aatgaggcag aattgaaaag
    67381 cggttgaaag acaggtagtg tctcagtttg aatttcttac cattcaattg gcttattaag
    67441 tacaaatcca tctggctttt gaactctgtc ttcaagctgg ctttctttca gcttctttcg
    67501 cccaattatt tgagaacctt ttgccacctg agatacgcta ttattcattg taactatgaa
    67561 gaatacatat tgcggtaaaa cactgtccgt ccatgtttta ttgggtgctg ttagccattg
    67621 tgatttttgg tcgtttaatc tttgaaacta agccgtctat atgttcgaag aggtgaggct
    67681 ttattggatt ccacattgga tccaatagaa agtcaatacc ttccctgcga gctaattttg
    67741 cagccggaac gaaatcgctg tcaccagaaa ttaaaatgat ttgatccacc tgacctttta
    67801 aggtaattga agcaatatct aatcctatct tgatatctac cattttttgg ttaaagtcaa
    67861 attgcacgtc atcttccgtc aagtcttcta ttgtaatttg cttcgaaagt aactccttag
    67921 ttattgatgg tttaatgatc caccttttac gatcttctaa aattccaagg cgtagagcaa
    67981 tctttctctt cttctttaat tcgttaaaaa atgcacattg gaatctatac tgatcagttt
    68041 tagaaaaatc aatggactta cctgtcagtg ggttatgctg ctttttgtca tagggcagac
    68101 agtcataata aaaaatcctg tacaaatcgt aggtctcaga cttagcttgg gacaaatgct
    68161 tcaagcacat ttcccaaaga tcttttgccg tcctttcggg gttaaggttc ttgactttat
    68221 ttatggagcg ataacgttta acaaaaaacc ctccatcaac taaaattgct gttcttcttt
    68281 tcatcttatt caaaaaaaaa gccctggggt cggcattctc gctattttat gaagaattga
    68341 gaatacgttg ccaagagcgt tatatggagc aaatgtatag atattagtct aagactgcaa
    68401 gtcaataata ctttcaccga gttcttataa tggctgaaag gaaaaatcaa tagatttatg
    68461 ggatgaacca cccaaccctc aacaagatag aagaagcact caattcaatt ggaaattatg
    68521 tgcgacagac tccggtttgg gagtggaaag gttcggtgaa agacaatatt ttcggttcgg
    68581 atactacgat catactaaag cttgagcttt ttcaacatgg cggaagcttt aagactcgtg
    68641 gagctatggt gaatatgctt gctttaactc ctgaacaatt agagtgtgga gtaacggcag
    68701 tcagcgcggg aaatcatgcc attgcggttg cttatgccgc aaaactcctt ggttcatcag
    68761 ctaaagttgt tatgccatcg acggcgaacc cttaccgggt aacaaaatgc gaagagttag
    68821 gagcagaagt tgttttgatg gaaaatgttc accaggcttt tgcagaagca gaaaggatcc
    68881 agaaagaaga aggccggact tttgttcatc cctttgaagg atttaatacc gttcttggaa
    68941 cagcgacttg cggttatgaa tttatcaccc aggcacaaaa tttagatttg gcaattattc
    69001 ccattggcgg cggcggattg attgcaggga tggccgcggc tataaagcag gtgaatccaa
    69061 attgtaaagt gatcggagtg gaacctgaag gagcagactc catgacgcgc agccttaaga
    69121 gtggaaagcc tgaaggagtt gagaaagtaa caaccattgc cgacagcctg ggcgccccat
    69181 atgcattacc ctattctttt tccgtggtgc agaagtttgt ggatgaagtt atgctgattt
    69241 ccgaccagga aatgtgtgaa gcgatggcgt tgctttttac cgatgcaaaa ctggccgtag
    69301 aaccggcggg cgcggcttcc accgcggcgt tgaaaaaaat tgtgaaaagt taccagggga
    69361 aaaaaatcgg gctgatggtg tgcggagcga atatagatct ggataatttc tcgaagtatt
    69421 taaagcaggg tggactagca agaaatagta agtaatgaac cctcatcgaa tttaggttta
    69481 tggttcaaaa ataatatcgg ggagaaacgg tttgacaaat ctggcaccca ataattgcca
    69541 catcggaaga ttaaaattgg atgaaactta aaccaacttc ttaggatctt gtgtaaaagg
    69601 tgtgttttac cagaagcaag ttcatgcaag taaaaattac tatttcctgc ccgattagat
    69661 ttaaaaagca atttctttcc atccggagaa caggttgggc ttccatcatt aaactcattg
    69721 gaagttattt gccgaagatt gctaccatca ctgttcacca cataaatttc aaagtctccg
    69781 tccctgttgg aataaaaagc aatctgattt ccgtcgggag accaaaccgg atcatggctg
    69841 tcaacttttg attgcgccag ggactggcaa acaataaata agggaaatag gaggaaggct
    69901 gatttcatga gtaatgcttt tgtatgtttc cagctataat tttgatttaa aataagtctt
    69961 tttcttgcga tttatcttac gcgaataaat tagaagttag gtatttattc gattcaatcg
    70021 atcgcttacc aaataatatg gtgatattta gataggttaa taaggacaca taatggccaa
    70081 cctcagaaaa gtattcatac tgctaatggg gctttctgtc tttactattt cttgcaaaag
    70141 agatgcggag ccactccccc ctttcatagg aacctgggac cttagaaaac tggttgtgaa
    70201 ggattgtgta gatccggcag aaaatgacga atacttgtgc cccggaggtg gttgcgatat
    70261 tatcgtattc tcaagccaaa aagttcgcct tgatggaacc gcctatgact atactgttat
    70321 gggtgaaatt atacatgtta atctatctcc aagtattata ctggtaatac aatggaaaat
    70381 tgttattagc acccttacat taatcattaa ggatagtgtg gccgatggcg gttgcacttt
    70441 cgaatacact tacctgaaaa cctgaaggtt ctcaaagctg tacatgatta taaaaaatgg
    70501 gcgtgtcccc ctcaacgggg gccgggctgc tccgggctcg ctgttcgctc ggccccgcca
    70561 gaagcggggc taaaccctgc gcatccctca cgcaaatcgc cacctaaatt ttatccctga
    70621 atttccccgt gcttcaaacc ctgaaccacc gctgccgctt gtttcagaac gcgtaccatc
    70681 aataacaaac gtgaccttct cctcaaatta ctttacgggg ttggttgggg ttgtacatag
    70741 acatcgcctt aaaacggcca gtcttaaaaa aaatcagatt tatcagggag cctgctgccc
    70801 aaaaaattcg tagttcaggg gaatatattt cttccactct tccggaactt cactttcttc
    70861 gaaaatagca tccacagggc aggccggaac acacgcgtcg caatcaatac aaacctcagg
    70921 atttatataa agttgttttc ccgcggggtc aaatcccggt tcttttactt ccgcgccact
    70981 accttctgta ttcacaggtc cgtggataca atctaccggg caaacatcga cacaagctgt
    71041 gtcgcaagta ccgacacaag gctcacaaat tatatatggc ataatataac aaggtttact
    71101 tttctaaatg attaatgtca tttgtcaata taggaaataa aaataatatg cactcgaatt
    71161 atagtcccct aattggtatt ctgcctttag aggggtaaat ttaaagaatt tgcgaaagat
    71221 attaggcaaa atttgatatg agcgagacct tagcaaaaga aaaacaatcc caagagctcg
    71281 agaaagtaac aatacgcttt gccggagact caggtgatgg aatgcagttg acgggttcac
    71341 aatttacatt cacctctgca atgttgggaa atgacctggc caccttcccc gactttcccg
    71401 cggagattcg ggcaccccag ggaacagtgg aaggcgtttc cggctttcag gtgcaaatag
    71461 gaagaattga tatttacacc cccggagatt tggccgatgt tttggttgcc atgaaccctg
    71521 ccgccctgaa atcaaacctg gaaaatgtaa aaaaaggtgg aaatattatt gtagatcaag
    71581 acgcttttac ctcaaagaac tgggaaagag ttggatataa agccaatccc gtcgaagatg
    71641 gatctcttgc ggattacaat attgttaatg ccccgatttc cagcatgact aaaactgctt
    71701 tggaaggact ggatatagat aacaagagcg tactgcgctc aaaaaatatg ttcgctcttg
    71761 ggatgatgta ctggcttttc gatcgttccc tgaaagagac agagaaattt ttggatgcta
    71821 agtttaagaa aatgccagtt ctggcagaag caaataaaaa ggctttgtat gccgggtttc
    71881 attatgctga aaccgtagaa gcattacctt catcttataa gattccgccg gcagtaattg
    71941 aaaaaggaaa atatcgtcat atttccggca atgtagccac cgcctgggga ttgatggcag
    72001 ctgcgcaaaa aatgaaaaag aaattattct atggaactta tcccattacg cccgcatcag
    72061 atattctcca tgaactttca cggtataaaa atatgggagt aattaccctg caggcagaag
    72121 atgaaatagc cgcagtttgc agttctattg gtgcaagcta tgccggccaa ttgggagcaa
    72181 ctgcttcctc agggcctgga attgcgctta aaggggaggc aatcggatta gctttaatgg
    72241 ccgaacttcc gctggtaatt attgatgtgc agcggggagg tccatcgacc gggctgccta
    72301 ccaaaactga acaggcagat ctcaaccagg ccatctatgg aagaaacagt gaaagccagg
    72361 tcatagtttt ggcggcaagt acgccaagca attgctttga tttcgcattt gaagcttcaa
    72421 gattatccgt agaacatatg acgcctgtga ttttactcac ggatggttat ttggcaaatg
    72481 gatcagagcc ctggaagttt ccttcaatgt cagagttgcc agatatcaaa gttccggcga
    72541 ttaatgtaaa agataacggc cagcccttct tcccatatag ccgcgatccg gaaactttgg
    72601 tacgtaaatg ggcagttccg ggaactccag gcctggaaca tagaattggc ggacttgaaa
    72661 aacaagatca aaccggtaac gtgtcgtatg aggcagcgaa tcatgaaatg atggtaaaac
    72721 tacgggcgga taaggtcgcg aaagttgcca atagaatccc agaccaatcc gtgtatggtt
    72781 caggatcagg gaaactccta gtggtcggtt ggggtggaac attcggcgcg ttatatactt
    72841 cagttaaaga cgcccaggag gttggaaaag atgttgacct ggcacacttt aattatattt
    72901 tcccgctgcc taaaaacacc ggagatttac tttcccggta cgagacgatt ttggtttgtg
    72961 aattgaatct cggtcaattt gtagggtatc tccggtctaa attccccggg ttaaatttta
    73021 agtcttatac aaaagtgcag ggtcaacctt tcacaataag tgagttgaaa acgcagtatg
    73081 atgaaaattt gaaataatta tgagcaacat agacataaaa cccttaacca aaaaagattt
    73141 cgcgagcgat caacttgttc gctggtgtcc cggttgtggt gattactcca tactttccgc
    73201 aatgcagttg gcgcttccgc agttgggatt aaaccgggaa gatattgttt ttgtttcggg
    73261 cattggttgc gctgcgaggt ttccttacta catgaacacc tatgggtttc atacaatcca
    73321 tgggagaggt cccgccgtag ctaccggcgt taaacttgct aatccaaaat tgagtgtttg
    73381 ggaaattcag ggcgatggag acgcgctggc tatcggcggt aatcatttca tacatgtctt
    73441 acgcagaaat gtgaacatca acattctcct gtttaataat gaaatttatg gcttgacgaa
    73501 aggacaatat tcaccgacct ccagaccagg tcttgttacc aagtctactc catttggtgt
    73561 aattgacagg ccctttcacg ctgcggaact ggtaataggt gctcagggaa ctttttatgc
    73621 acgaaccata gacacgaatc caaagttgat gtctgaaatc tttgtgcatg cagccggtca
    73681 tacaggaact tcaatggtag aaatacttca gaactgtgtg atatttaatg ataaaactca
    73741 cggcgcgata accagtaagg aaaatcggga tgattaccag ttacaccttg aacatggcaa
    73801 gcccatgctt tttggtaaag acaacagtaa aggcataaag ttaaatggct tgaaactcga
    73861 ggtagttaac ctgggtgaaa atggaactat tgaatcagat attttgattc atgatgcgta
    73921 tgatgaggat ccgactttgc atttaatgct tgcccgtatg catccacccc ttttcccaat
    73981 ggctttggga gtgataagag aggtaagaaa tactacctat gatgagttgg tggaaagtca
    74041 gtatcaaaat agtgttaata atgcaaagtt ctctaccatg gatgagttgt tgaatagtgg
    74101 cgaaacctgg gaggtgaaat aatggaagca caggcgctgg taatattctt tctggctggt
    74161 gttgtaatgg ttggagcagg cgtttttgtc tcataccttg tatcaccaaa gtcagaaaat
    74221 tctcagaagg gagaaaccta tgaatgcggt gtgccaacga ttggccaatc catgattcaa
    74281 tttaaggtag gatactactt gttcgccatt ctttttttaa tctttgatgt tgaaactgtt
    74341 tttattttcc cctgggctgt ggtgatgaaa tcagtgggga tggcggcgtt tattgaaata
    74401 ttaatctttt tagtgatttt aggattaggc ttattatatg cctggaaaaa acaagcattg
    74461 aaatgggatt agaaggtata ggtatcgatc caaaaacccg ggaaattttg aaagatttcc
    74521 ccggtgaaat aaagaaaggc ccggggagta atatcgtaat tacctcggta gatggaatag
    74581 tgaattgggc aaggtccaat tcactttggc cattggtatt tggaactagt tgttgcgcga
    74641 tagaaatgat ggcaactggg gcaagtcgtc atgattggtc cagattcgga acagaggttg
    74701 cccgggctac tccaaggcaa gcagatctta tcgtgattgc cggaacaatt gtaaataaaa
    74761 tggctccggt gctgaaacga ctttacgatc agatggctga tccaaaatat gtaattgcaa
    74821 tgggcgcctg cgcaacatca ggcgggccct tttattacaa tacatattcg gttgtgaaag
    74881 gtgccgacca tgtaatcccg gtagatgttt acgttcctgg atgccctccg cgaccagagg
    74941 cattactgta cggaataatg caattgcaga aaaaaatcaa aacagaatca ttggctaaca
    75001 gaagagaccc gatgaaagat tttatttagg tagaaatatg gacaatgaaa agctaaaaga
    75061 ttttatacta aagcaaattc ccgcagcaga cttcgaggaa aatgcccagt atcttttggc
    75121 aatagtcccg gcatctcaca ttcattccat ctccctggaa ttaaaaaata atcctgaaac
    75181 cagatttgat tacttattct gcctgaccgg cgttgactat cctgatcaca tgcaggttat
    75241 atatcatctg aaatcaacag aattgaatca ctatacggtg attaaagcta gaattgatag
    75301 cagagaaaaa cctgaaattg ataccgtttg tgatatttgg catacggcgg aatttcatga
    75361 aagggaagta tatgatctct tcggaatcgt cttccggaac catcctgatt taagaagaat
    75421 ttttctggat gaagaatggg taggctatcc gttaaggaaa gattataaag atccctataa
    75481 tatgattgaa tactaatgaa ggaaatagag gaacaggaga tacaggaact ggaatcgcag
    75541 gaatacttca taaatatggg gccgcagcac ccgtcaactc acggggtgtt gcgattggtt
    75601 ctttcattgg acggggagat tatcagaaaa gttgagccgc acctcggcta tatacaccgc
    75661 tctattgaga aaatgtgtga gcacgacagc tatcagcaaa tcattcatct tacagatcgc
    75721 atggattacc tttcctgtca tatgctcaac gaggccgttt gccttggagt tgaaaacggc
    75781 ctggaggtgg aaatacccga acgggtaaaa gtgataagaa caatcatttc agaattaaca
    75841 agattatcct cgcatcaact ctggtgggga gtaatgggta tggatttggg ggcaattact
    75901 actttccttt atggcttccg tgaccgtgaa atgattacag atattttcga ggaaacttgt
    75961 ggcgccaggc tcaccatgag ttacaacatt cccggcggtt tgatgttcga tattcatcct
    76021 aactttcaaa agaggactaa agattttatt gttcatttca agaaaaaact ccctgaatac
    76081 gacaaacttc tatctgataa tgtgattttc cgagaaagaa ccagaggaat cggcgtactg
    76141 tctaaggaag atgcaatttc ttacggtgtc accggaccat caggacgtgg atcaggtttt
    76201 tcatgcgacg ttcgaaagca caacccatac agtgcctatc ctaaagttga atttagagaa
    76261 atcctgaggg aagagggaga ttgctttgcc cgatataaat gcagaattga tgaaatgtgg
    76321 gaatccttga atattatcga ccagctcatt gacaatattc cggagggaga ttataaagtt
    76381 caaacaagaa cagtaataaa attacccaaa ggagaatttt accagaaagt ggaaactgcc
    76441 cgcggtgaat ttggagtata tattataagt gatggaatga aaagccccta taggctgaaa
    76501 ttcaggtcgc caggttttag caatttgtca gcccttgata aaatgtccag aggatgtaaa
    76561 attgcagatc ttgtggcgat aatgtcaacc ttggatttag taatccctga catcgacaga
    76621 taatgaaatt tttatatgac ttcggttatt taacaggttc catccatgaa tggctatctg
    76681 ctcgcatgga tccgccggta gttaccttgg ttgaaaccat catcgtagcc gtttgtttca
    76741 tgatattatt tgcagtgctt ggattagtcc tggtgtatgc agaaagaaaa atcagtgcat
    76801 tttttcaaca gcgattggga cctatgcggg ttggaaagtg gggtacagcc caaacaattg
    76861 ctgatgtgat taagctatta tttaaggaac cgctggtgaa taaagatgcg gataaatttc
    76921 tgttcaacct tgcaccttat ataataatga tttccgcatt tctggcaatc gcggcattac
    76981 catttgcaaa aggccttcat gcattggatt tcgacatcgg gatattttac gtcagtgcag
    77041 tttcttccct gggtgtaatt ggtatcttgt tagcaggctg gtctagcagc aataaatatt
    77101 ctttgattgg cgccatgcgc tcgggagccc aaattataag ttatgaactt tcagttggac
    77161 tttcattact aacaattatt gtactgacag gcagcttaca attatcaaca atcgtggaat
    77221 cccaggccga cggatggtgg atttttaaag gtcatttgcc tgctgtcatt gcatttctga
    77281 tttttatgat tgccagcaca gcagaaacga accggggacc atttgattta gcggaagcgg
    77341 aatcagaatt aacagcagga tttcatacgg aatattccgg aatgaagttc gccttctttt
    77401 tcctggccga gttcgccaac atgtttatag tagctgcaat cggagctact gtatttcttg
    77461 gcggctggat gcctttttat atcggaggat ttaccggatt taatgcagtt atggatttca
    77521 taccgccaat tttctggttt ttaggcaagg tagcttttat gatttttatg atgatgtggt
    77581 ttaggtggac tttcccaaga ttacggattg atcagttatt gactttggag tggaaatacc
    77641 tcctgccaat aaatcttgtg aatatagttg taatggcatt tatcggatta atgggctggc
    77701 atttttaatg tgttagataa tgaacggaat aataaaatat tttaaagaca tttattacgg
    77761 actgaaatca ctggttaccg ggatggcagt gactggccat tacattaccc atcctaaaga
    77821 aattatcacc cagcaatatc ctgaaaataa ggataccctg aagatgtttg accggttcag
    77881 gggagaggtt gtacttattc atgatgaaaa taatgaacac cgttgcacgg gctgttccgc
    77941 gtgtgaagtg gcatgtccta atggcactat tgaaatcatc agtaagagag tagaaaatga
    78001 agaaggaaag aaggtgaaag tgatagataa attcgtatat cacttacaga tgtgtaccat
    78061 gtgtaactta tgcattccgg cttgcccgac agacgcgata aaaatgtcgc aggattttga
    78121 acacgcggtt tttgacagat caaaattaac caagatcctg aataagcctg ggtccaaaat
    78181 aatggcggga gttaaatgag atagctttgg aaacatactt tttttatttt atcgccgcct
    78241 ctatgttggt attttcctta ctggcagtca cctcaagaag aattcttcgc gctgccgttt
    78301 atcttttatt cagccttgcc ggcacggccg ctttttattt catgatcgat tattttttcc
    78361 tggcagcagt gcaattaatt gtatatgcag gtggaattgt ggttctcata atattttcaa
    78421 tcctgctaac cagccatata gcagataaat tggaacggcc ggatatgatg aagaatgcat
    78481 tctccgcagt tcttgtttta ggaggaatcg cgctaacggc ttttgtactt ggcaattttg
    78541 ccgatggtgc gccttcagca acaaatcaac tcaacatgca aatcataggt gagaaattaa
    78601 tgaactacaa ggagaatgga tatgtactcc catttgaagt gataagcatt ttattgctgg
    78661 cggcaatggt aagcgcaatt gtggtggcaa aacgtactaa aaccaaaaat gatgtttgaa
    78721 aatattcccc tcaaccatat tctgatactc accatctcta tgttcttcat tggtgtatat
    78781 ggttttctca cccgaaaaaa tcttgtaaca gttttaatca gcgtggaatt aatgttgaat
    78841 gctgtgaaca ttaattttgt tgcctttgat aaatacctcc atcctggaac atttgaagga
    78901 cacttcttct cattatttgt aattgcaatc gccgctgcag aggcggcagt agctatcgcc
    78961 atatttttta atctctatag aaacatcaga tcaattgatg tcgaaagcgt tgataaaatg
    79021 aagtattaat gaattttgaa tacacaattt ggattctttt attgcctttt ctggctttta
    79081 caatcaatgg gctgggaagt aaaaaaatgg gacttgcttc cgcctggatt tcaattactg
    79141 gaattggcat tggagcggca ttatcgctta ttgcagctta tcaatatttc accaaagaat
    79201 cagaaatatc cagcttctac ccatacgatg ttacctggct gaaatttaca gactctctta
    79261 gtataaatat gggaattttg gtggatccaa tttcagtgat gatgctggtg gtgattaccg
    79321 tagtttcact catggtgttt atctatagtg ttggatatat gaaaggtgaa tccggctatg
    79381 cacgatactt tagtttcctt tctcttttca gcttttctat gctcggactg gtaattgcaa
    79441 ccaatttatt ccagatgtat attttctggg agctggttgg tgtatcttct tatctgttga
    79501 tcggttttta ctatacaaaa gc
//
LOCUS       DASWHX010000016         3405 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_749420, whole genome shotgun sequence.
ACCESSION   DASWHX010000016 DASWHX010000000
VERSION     DASWHX010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 3405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3405
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_749420"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..857)
                     /locus_tag="VGA21_01040"
     CDS             complement(<1..857)
                     /locus_tag="VGA21_01040"
                     /inference="COORDINATES: protein motif:HMM:NF024371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HEX9649117.1"
                     /translation="MRLKIKGYLVVVIIFLANPVPGIGQSAVNLSDYQSRYPENNAIY
                     LDYLKEITIDMEGDSIKIHSEQLERVLHLNSNSRYLAKDKVFSSFFVKTKSIDAQTIV
                     PQGSEFTIMKVEDFLEKDESSSGVFYDDSKSTNFIFPGIKPGSITQLKYQQQILDPRF
                     LNSFFFQSFIPIEKAQLVLKVDKNISIAYKMFHQGNLEIDFSESKKGKYTIYKWTSRN
                     VKAVKYEEDSPNLSYFAPHLVYYISKVKKGTEEHVILGEVKNLFDWYSTFTSHLDSEE
                     SPDLRKTVEG"
     gene            965..1384
                     /locus_tag="VGA21_01045"
     CDS             965..1384
                     /locus_tag="VGA21_01045"
                     /inference="COORDINATES: protein motif:HMM:NF014173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEX9649118.1"
                     /translation="MQANGRFTIAIHICLFLAHQEKKLIPSQEIAASVKTNPVVIRRL
                     IGKMRGNEIIGSVAGAKGGFYLAKKPEEITLWEIYNSVKDGDFFFKHKPNEDCPVGGN
                     LCELVDEVYKYAESSMEQVLGKVTIFDLHEKLNTALV"
     gene            complement(1481..1876)
                     /locus_tag="VGA21_01050"
     CDS             complement(1481..1876)
                     /locus_tag="VGA21_01050"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX9649119.1"
                     /translation="MKLELKAIRAQREKWIEAVNNGDIDAISEIMSDDAVWMPASIPA
                     LNGKKAILDWIKPSIDNFDHQFSISHQDLKGAGNWVVEHADFSHSLTPKDGGETTEFP
                     GSYIVIWRWEKDNVWRIERYVDNSNVLVE"
     gene            2167..2751
                     /locus_tag="VGA21_01055"
     CDS             2167..2751
                     /locus_tag="VGA21_01055"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018472339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEX9649120.1"
                     /translation="MTKKIRCGWCEGSFEEYITYHDEEWGVPVHDDKKHFEFLILEGA
                     QAGLSWSTILKRRKGYRKAFANFDAAKVARFNETKIQKLLQDQGIIRNKLKVRGAVKN
                     AKAFLQLQKEFGSFDNFIWSFVGDKPKVNRWKSMKQLPANSKESDSLSKELKKRGFTF
                     VGSTIIYAHMQACGLVNDHLTNCFRYNQIIKKNK"
     gene            2753..>3405
                     /locus_tag="VGA21_01060"
     CDS             2753..>3405
                     /locus_tag="VGA21_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007660625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5117 domain-containing protein"
                     /protein_id="HEX9649121.1"
                     /translation="MKSKKIILFLVMFFAVNSMLAQRNKQNSPQLQAQSGIASKVAGM
                     IKHDGYFVFYLEEKTHKIFLEIDKLDAEFLYINSLPAGIGSNDIGLDRGQLGGERIVK
                     FVRYGPKVLMIQPNYDYRAISQNELERKAVEQAFAQSVLWGFKVEAEDGGKVLIELTD
                     FIMQDAHNVVGRLRSSRQGNYKLDASRSALYPERIKNFPQNTEFEVTLTFSGTPEGG"
BASE COUNT         1085 a          632 c          663 g         1025 t
ORIGIN      
        1 ccttcaactg tttttcttaa atcaggactt tcttcactat ccagatgaga ggtaaaagta
       61 gaataccaat caaacaaatt tttcacttct cctaaaatca cgtgctcttc agttcctttt
      121 ttaacttttg aaatataata aaccaggtgc ggagcaaaat aactgagatt tggagaatct
      181 tcttcatatt ttactgcctt tacattcctg cttgtccatt tataaatcgt gtacttacct
      241 ttcttagatt cagagaaatc gatttctaag ttaccctgat gaaacatttt gtatgcaata
      301 gaaatatttt tatcgacttt tagaacaagc tgagcctttt caataggaat aaaagattga
      361 aagaaaaagg aattaagaaa tctgggatcc aatatttgtt gttgatactt tagttgggtg
      421 atggagcccg gcttaatccc agggaaaatg aaatttgtgg acttcgaatc atcataaaag
      481 actccggaac tgctttcatc tttctctaaa aaatcttcca ctttcattat agtaaattca
      541 ctaccttgtg gaacgatcgt ttgcgcatca atggattttg ttttaacaaa gaatgaactg
      601 aaaactttat ccttggcgag gtatctcgaa ttggaattca ggtgcaacac ccgctccaat
      661 tgttcagaat gaatcttgat ggaatctcct tccatgtcaa tagtaatttc cttcaagtaa
      721 tccaggtaaa tggcgttatt ctccggatat cgtgactggt aatcagaaag attaaccgca
      781 ctttgcccaa ttcctggtac cggatttgcc aggaatatta ttacaacaac taagtatcct
      841 ttaattttta gcctcatacg cgagctctaa ctgccccaaa tttagtacga acaaagctaa
      901 catcaatatt tagctgttgt tttgtaactc ttacggttac atttcgtaaa ttgttgtgaa
      961 aattatgcaa gcgaacggaa gatttaccat tgccattcat atctgcctct ttcttgctca
     1021 ccaggaaaag aaattaattc caagccagga gattgctgcc agcgttaaga cgaatccagt
     1081 tgttatccgg aggttaatcg ggaaaatgcg aggtaacgaa ataatcggaa gtgttgcagg
     1141 agcaaaagga ggattttacc ttgcaaaaaa acctgaagaa ataactttgt gggaaatcta
     1201 caattcagta aaagatggag attttttttt caaacataaa ccaaatgaag attgtcccgt
     1261 tggaggtaac ttatgtgaac tggtggatga ggtttacaaa tacgcggaat cttctatgga
     1321 acaagtattg ggtaaggtga caatctttga tttacatgag aagctaaata ctgccctcgt
     1381 ataacctgga taaaactttc ttaaccttta atataactcc ttattctcag gaggtattgt
     1441 aattaacaaa ccaaaaatta ttggcaactg ccggggttaa ttactccacc agaacattcg
     1501 aattatcgac gtaacgctca attctccaaa cattatcttt ttcccatctc cagataacaa
     1561 tataactacc ggggaactcc gtggtttcac cgccgtcttt cggtgttaat gaatgactaa
     1621 aatcagcatg ttcaaccacc caatttccag cacccttcaa atcctgatgg gaaatagaaa
     1681 attgatgatc aaaattatca atggaaggct ttatccaatc caaaatagct ttttttccat
     1741 tcagagccgg gatagaagca ggcatccata cagcatcgtc tgacataatt tctgaaatgg
     1801 catcaatgtc accattattt acagcttcaa tccatttttc ccgctgggcc ctgatggctt
     1861 tcaattctag tttcatattc cgttaaagtt taaggtttat aatgaggtaa gacctgatta
     1921 tgctgcacat aacaagctga attaaatttt cattaatata attaaaatcg gaaataatcg
     1981 aagtgataaa aggcattttg tgatcttttt ttgttgaaaa caatgataaa atcaggatgc
     2041 aacaagtctg atgtgttccc attcaattcc tgcattttgc ttttggttat taaaacgatt
     2101 cctgatgtaa atctggtatg aataccggtc attttttggt acattaaggc cctttctgcg
     2161 ataaatatga caaaaaaaat ccgctgtgga tggtgcgaag gttcctttga ggaatacatt
     2221 acctatcacg atgaagaatg gggcgtgcct gttcacgatg acaaaaaaca ttttgaattt
     2281 ttgattcttg aaggagcaca ggcgggtctc agctggagta ccattcttaa aaggaggaag
     2341 ggataccgga aagcattcgc caactttgat gccgctaagg ttgcacgatt caatgaaacc
     2401 aaaattcaaa aattgcttca agaccaggga ataatccgta acaagctgaa agtgcggggg
     2461 gcagttaaaa acgccaaagc ctttcttcaa cttcaaaaag aatttgggag ctttgataac
     2521 tttatatggt catttgttgg agataagcca aaagtaaacc ggtggaagtc catgaaacaa
     2581 ttaccggcca attccaaaga atctgattca ctaagtaaag aattaaaaaa gcgtgggttc
     2641 actttcgttg gcagcacaat tatatacgcg cacatgcagg cttgtggtct tgtcaacgac
     2701 catctgacga actgcttcag atataatcaa attatcaaaa aaaataagta gtatgaaatc
     2761 aaaaaagata attttattcc ttgttatgtt ctttgctgta aattcaatgc ttgcacagcg
     2821 gaacaaacag aattcaccgc agctacaagc ccaatcgggc attgcttcaa aggttgcggg
     2881 catgatcaaa catgacggtt attttgtgtt ttatcttgag gagaagaccc ataagatatt
     2941 tctggaaata gataagttgg atgcggagtt tctctatatc aattctctcc ccgccggtat
     3001 tggttcgaat gatattggat tggatcgtgg ccaactgggt ggtgaaagaa ttgtaaaatt
     3061 tgttcgctat ggacccaaag tgttgatgat acaacccaac tatgattaca gggcaatctc
     3121 ccaaaacgaa ttagaaagga aggctgttga acaagcattt gcccaatctg ttttatgggg
     3181 ttttaaagtg gaggcagagg atggcggcaa agtcttaatt gaactgactg atttcattat
     3241 gcaggatgct cacaatgttg tgggccggct gcgcagttca cgccagggaa attataaatt
     3301 agatgcgtca cggtcggcac tctatccgga gaggataaag aattttccac agaatacgga
     3361 atttgaagta acattaactt tttcaggaac tccggaagga ggctg
//
LOCUS       DASWHX010000017         2261 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_2228562, whole genome shotgun sequence.
ACCESSION   DASWHX010000017 DASWHX010000000
VERSION     DASWHX010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 2261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2261
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2228562"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            66..>2261
                     /locus_tag="VGA21_01065"
     CDS             66..>2261
                     /locus_tag="VGA21_01065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649122.1"
                     /translation="MRKTFTCFVLLLAFSPAFSQKQISKGKRTGAPEIPVMQSKKIST
                     GIVEYAGGQGTGIYIPPPKEFLQRRNQRTTANDLFNVTYNGFPDSVRQAFQFALDIWA
                     TLVTSEVPITVEANWVQMESSVLGSALPGTAYTNIFYSRKFDAWHPVSLAEKIIGENL
                     NETDPDIIANFNNSVKWNTDTLGNPAPDEFDLVTVVLHEICHGMGFLDFTDVEAGFGF
                     YNFQEKPWIYTTFLENTNGENIVNTYDNQTMALANQLQSDKLVFHEKISLAKYNNNLT
                     KVPIYAPLTYEPGSSISHIDQDVPSFHGLMRPGISKGDAIHNPGVAVNMLEDMGWALT
                     FINHDGTPNTEDVDNPKTVTANITSDTDVDSASFILVYSTEFENDPFTTPSLTDTVQF
                     ASVLQGNFEAMVPDFNGTPTLYSYYLVIKDEYGRVFTNPPEAPEFYYQFGASPDDFPP
                     SLTHNPIFFVEQGTVTVPVSATVTDFIGVNSVSIEYQIDGAPQGSFDLILDPNSSSLY
                     KGSFTLSASLPVSTVINYKITATDDAMAQNQSFFPESGFQSFTIIEPVAPVKVYTNNF
                     NETGAGDDFVGNLFSVQNVVGFSSPAFHSPHPYGTGPVNDAVEYITELKIPIILDNDS
                     AFIQFDEVVLVEPGDPGSVFGNIRFWDYVIVEGSKDKGASWLPFGNGYDCRANSNWFD
                     TYNSDFDPSTLNSGASGDSDLYADRKIDMLANGNFSADETVRIRFRLWSD"
BASE COUNT          634 a          467 c          487 g          673 t
ORIGIN      
        1 tttatggtta ccgaatagat taatcaggtt attaatatct tgttgggggt aattaaatct
       61 aacttatgag aaagaccttt acttgttttg tcctacttct tgccttttca cccgccttct
      121 cacaaaagca aatctctaaa gggaaaagaa ctggagcgcc agaaattccg gtaatgcaaa
      181 gcaaaaagat tagtacggga attgtagagt atgcaggagg ccagggcacc ggtatttata
      241 ttcctcctcc taaagaattt ttacaaagaa gaaatcagag aacaacggct aatgatttgt
      301 ttaatgtaac atataatggc ttcccggatt cggtgaggca ggcttttcaa tttgctcttg
      361 acatttgggc cactcttgta accagcgaag tccctattac cgtggaagct aactgggtgc
      421 aaatggaaag tagcgttttg ggatcggcac tgcccggcac agcttatacg aacatttttt
      481 acagtagaaa attcgatgcc tggcatcctg tgtctctggc ggaaaaaatc atcggagaaa
      541 acctcaatga gactgatccc gatatcattg caaactttaa taattctgtt aagtggaata
      601 cagacactct tggcaaccct gctccggatg aatttgattt agttaccgtg gtgttgcatg
      661 aaatatgcca cggaatggga tttctcgatt ttacggatgt ggaagccggc ttcggctttt
      721 ataattttca ggagaaaccc tggatatata ccaccttttt agaaaacaca aatggtgaaa
      781 atattgtaaa cacttacgac aatcaaacta tggcgctggc taatcagttg caaagtgaca
      841 aattggtttt ccatgagaaa ataagcctgg ccaaatacaa caacaacctt actaaggtac
      901 ccatttatgc acctcttact tacgaacccg gatccagcat ctctcatatt gatcaggatg
      961 tgccttcttt tcatggactg atgaggcccg gcatctccaa aggtgatgcg atccacaatc
     1021 caggggtggc ggtaaatatg cttgaagata tgggttgggc attaaccttt atcaatcatg
     1081 atgggactcc aaatacagaa gatgtggata accctaaaac agtgacagcc aatataactt
     1141 ctgacactga tgtagatagc gccagcttta tccttgttta ttccactgaa tttgagaatg
     1201 accctttcac tactcccagt cttacagata cggttcagtt tgccagtgtt ttgcaaggta
     1261 attttgaggc aatggtgccc gacttcaatg gtacaccaac gctttattct tattatttag
     1321 taattaaaga tgaatatgga agggtattta ccaaccctcc ggaggcgccg gagttttact
     1381 accaatttgg agcttccccg gatgattttc ccccttcact cacgcataat cctatatttt
     1441 ttgtcgagca gggaacagtt actgttccgg tatcagcgac agtaactgat ttcattggcg
     1501 taaattcagt ttctatagaa tatcaaattg acggcgctcc ccaggggtca tttgacctga
     1561 ttctggatcc taattcttca tcattataca agggctcctt tacactttcc gcgtcacttc
     1621 cggtgagcac agtcatcaat tacaagataa cagcgacgga cgatgcgatg gcgcaaaatc
     1681 agtcattctt tccggaatct ggttttcaaa gttttactat tattgagccg gtagcgcccg
     1741 taaaagtgta cactaataat ttcaatgaaa ccggcgccgg tgatgatttt gtcggtaatc
     1801 ttttcagtgt tcagaatgtt gtaggatttt caagtcccgc ttttcactct ccgcaccctt
     1861 acggaacagg gccggttaat gatgctgtcg aatatattac ggaactaaag attcctatta
     1921 tactggataa tgattccgca tttattcagt ttgatgaggt tgtactggta gaaccaggag
     1981 atccgggatc tgtattcgga aatatccgtt tctgggatta tgtaattgtg gagggatcaa
     2041 aagacaaagg ggcttcatgg ctgccttttg gtaacggata tgattgcagg gctaattcaa
     2101 actggtttga tacctataat agcgattttg atccatcaac tcttaattcc ggagccagcg
     2161 gcgattccga tctgtatgca gatcgtaaga tagacatgct cgccaatggt aatttttcgg
     2221 ctgacgaaac ggtgagaatc cgctttcggc tttggtctga t
//
LOCUS       DASWHX010000018         1847 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_3336198, whole genome shotgun sequence.
ACCESSION   DASWHX010000018 DASWHX010000000
VERSION     DASWHX010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 1847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1847
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3336198"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(8..1510)
                     /locus_tag="VGA21_01070"
     CDS             complement(8..1510)
                     /locus_tag="VGA21_01070"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX9649123.1"
                     /translation="MAFNEKNVRLVFGLKLRQSRLDKKFSLSELSKKCKISISYLNEI
                     EKGKKYPKTEKIIALSKALDVSYDYLISFQLNKKLAPISELIQSNILEELPLDFFGVE
                     ASDLIEILSVAPTKLSAFINSIIQISRSYDIRVEHFFYSALRSYQEMIDNYLEDTENA
                     AAEFIRKFDVGYKGIDRIDLFRSILINEYGYTIEEDSFGDVKDLETLRYLYRPDCKIL
                     MLNEKINKNQYLFILGREIGYNFLGITDRSFTSSWVEVKKFDHILNNFKAYYFSSAII
                     LHEKNFVKGLIEFSTNVEFDPERLVDLMESYNSNAEMFMLRITNLIPRFFKFHEMFFF
                     RYNHDLTTNTFSLTKDLLLSGLHKQHGMISDFQPCKRWSGITILKELSILQEKNEYKR
                     PICRINRVKYSQTTREYLIISVAHPMYPTQNMNCSINVGFQINEKLLRRIKFLNDPKI
                     PFTEIDLDWIIRNSGNCEDDLEKPSVMIKESKTRKIREAVNELLSGQLVS"
     gene            1593..>1847
                     /locus_tag="VGA21_01075"
     CDS             1593..>1847
                     /locus_tag="VGA21_01075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649124.1"
                     /translation="MVWIIFARMFNELIKKLQERFKERELPGVEAHKKLAPFRTENSR
                     FNYSEIKGYKESSVLVLIFPQNGSAYIPLIKRPDYDGIHSG"
BASE COUNT          570 a          333 c          328 g          616 t
ORIGIN      
        1 gaatgcatta actaaccaat tgtccgctta acaattcatt aaccgcttct cttatttttc
       61 tggttttaga ttccttaatc ataaccgatg gtttttccag atcatcttca caattgcccg
      121 aattcctgat tatccaatct aaatctatct cagtaaatgg aatcttcgga tcattcagga
      181 acttaatcct tcttaagagc ttttcattga tttgaaatcc aacgttaatg ctgcaattca
      241 tattttgtgt tgggtacatt ggatgggcta ctgagattat cagatattct cttgttgttt
      301 gggagtactt gacccggtta attctgcaaa ttggtctttt atattcattt ttttcctgaa
      361 gaattgaaag ctctttaaga atagtaatcc cgctccatct tttgcagggc tgaaaatctg
      421 aaatcattcc atgctgttta tgaagaccgg aaagaagcaa atcttttgtc agagaaaaag
      481 tgtttgtggt aagatcatga ttataccgaa agaaaaacat ctcatggaac ttaaagaatc
      541 ttggaataag gtttgttatc ctaagcataa acatttccgc attgctatta taactctcca
      601 ttaaatctac aagccgctcc ggatcaaatt caacatttgt gctgaattcg ataagtccct
      661 ttacaaaatt cttttcatgc aaaataatgg cgctggaaaa gtaatatgcc ttaaaattgt
      721 taagaatatg atcaaatttt tttacctcaa cccaactgga ggtaaaggaa cgatcggtaa
      781 ttcccaggaa attgtagcct atttctcttc cgagtataaa taaatactga ttcttattta
      841 tcttctcatt tagcatcaga attttacagt ctggcctata caggtacctc aatgtttcaa
      901 gatccttcac gtctccaaat gaatcttctt caatggtata tccatattca ttaatcagga
      961 ttgacctgaa caagtcaatt ctgtctatac ctttgtaacc gacatcaaat tttctaatga
     1021 attcagcagc tgcattttcc gtatcttcca ggtaattgtc aatcatttcc tgatatgagc
     1081 gaagtgcgga gtagaagaaa tgctccaccc tgatatcata gctccttgag atttgaatta
     1141 tggagtttat gaaagcgctt aatttcgtag gtgctacaga taaaatctca ataagatcac
     1201 tggcttcaac accaaaaaag tccagaggta attcttccag aatattgctt tgaatcaatt
     1261 ctgaaatagg cgcgagtttc ttattcaatt gaaatgagat aagataatcg taggaaacat
     1321 ccagcgcttt ggaaagggcg ataattttct ctgttttggg atattttttg cccttttcga
     1381 tttcattaag ataggaaatc gagatcttgc attttttaga gagttccgac aatgagaatt
     1441 ttttatcgag tcgtgattgc ctcaatttca atccaaagac aagccggacg tttttttcat
     1501 tgaaagccat ttagaaagtt gattaggtta cgaatgcaag tgaaattagc aatttttgcg
     1561 aattttcgct aatacccaaa gacttaatga agatggtctg gattatcttt gcccggatgt
     1621 ttaatgaatt gattaagaaa ttgcaggaac gatttaagga aagggagctt cctggtgtag
     1681 aagcgcataa gaaattggca ccatttcgaa ctgaaaattc gcgattcaat tattcggaga
     1741 ttaaaggcta taaagaaagc agcgtactgg tgctgatttt tccccaaaat ggctctgctt
     1801 atataccatt gataaaaagg cccgattatg acggtatcca cagtgga
//
LOCUS       DASWHX010000019         4834 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_2969065, whole genome shotgun sequence.
ACCESSION   DASWHX010000019 DASWHX010000000
VERSION     DASWHX010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 4834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4834
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2969065"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            266..2620
                     /locus_tag="VGA21_01080"
     CDS             266..2620
                     /locus_tag="VGA21_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017729848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX9649125.1"
                     /translation="MRKLLILSALFIPIISVCQQLVSLNVDKIMRDPKWMGNFPGTPY
                     WSEDGTTLFFNWNPENNPDDSLYSFKPGVSNAPVKVGLVDRVNLPSRFGSYNRARTKK
                     IYQKDNSLILLDIPSKKKIVIIEGMGRVSNASFNHAETKVLFTMENNLISWEIATGTF
                     KQLTDFRTGNERKETKTSDQDIWLGDDQLAWLRVLKERKENREKSREMNNLEEPVSPK
                     TMYLGQSSIRNIQLSPDEKYITYMLSASANSKGTEVPTYVTESGYAEMLRARPKVGAE
                     GSNVEMKIYDIAGDTTYKVNVETLPGIEDIPDYYSDYPKYKDKKPEKRKVLPGSLNWS
                     PDGKYAFFEIRSQDNKDRWIGTLDVKTGKINVLDRQRDEAWIAGPGIGWSGFPGGNLG
                     WMPDSKRIWFQSEESGYSHLYTIDITNNKKTALTKGNYEIYDPVISADKKSWYFEANV
                     DHPGDVQVYKMPINGGKMEKLTNKPGRNDFYLSPDNSKIGISYSYSNQPTELYWMPAG
                     ANIMNQITNSQSEEYKSYPWRDPEIVTFKARDGAEVYARLYRPAIPQQNGPAVIFVHG
                     AGYLQNAHKWWSSYFREYMFHNLLADNGYTVLDIDYRGSAGYGSNWRTGIYRHMGGKD
                     LIDQVDGAKFLTEQYQVDANKIGIYGGSYGGFITLMAMFTEPGVFAAGAALRSVADWA
                     HYNHPYTANILNTPVEDSLAYARSSPIYFAEGLEGKLLMCHGMVDTNVHFQDVVRLAQ
                     RLIELGKDDWELAVYPLENHGFVEPSSWTDEYTRIFKLFEDNLK"
     gene            2688..4379
                     /gene="ggt"
                     /locus_tag="VGA21_01085"
     CDS             2688..4379
                     /gene="ggt"
                     /locus_tag="VGA21_01085"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009579422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEX9649126.1"
                     /translation="MKKILILAGICLFVSCQPMQETIESKQGLIAKNGIVVSAHPLAS
                     DVGKSILQKGGNAIDAAIAVQFALAVVYPVAGNIGGGGFMVIRKNDGSTYALDYREKA
                     PGAGSRDMYIGENGEVIQGLSLNGALASGIPGSVDGMAKAFEKFGTLKWSELVQPAID
                     FAENGYELTEREARGLNRNAESFEKHSTIRPDFILKNEWAAGEKILMKDLAETLMRIR
                     DYGRDGFYKGETAQLIVEEMERGNGIITLEDLANYEAQWRDPIVGNYKNYKMIGMPPP
                     SSGGVALLQLLGMVEGFPLSEWGWNSAKTVHLLVEAERRVYADRAAHLGDPDYYAVPV
                     SNLIDNNYLDSRMQNFNAELATPSDSIKAGSFQVYESTETTHFSIIDSDGNAVSVTTT
                     LNGGYGSNVIVGNAGFLLNNEMDDFSIQPGFPNMYGLIGGEANAIEPGKRMLSSMTPT
                     IVEKDRQLFMVVGTPGGSTIITSVFQTILNVIEFGMGMQEAVSSGRFHHQWRPENVFM
                     ESNAITSEDSAMLVKMGHKFLGSGGIGRVDAILVLGEEGLEGGADPRGDDKASGY"
     gene            complement(4376..>4834)
                     /locus_tag="VGA21_01090"
     CDS             complement(4376..>4834)
                     /locus_tag="VGA21_01090"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX9649127.1"
                     /translation="VYELVFSASGYLSRSEEIRVNPEEDLLALSVSLYPVELGSVVNL
                     DNVNFYRGTPDLIESSYKQLQIIAQLMIDNPNMEIELSGHTDNQGNSKLNLKLSQDRV
                     DKVKEYLILNGISAVRISGRGYGGTQPIASNASEETRKFNRRVEFIIVRN"
BASE COUNT         1451 a          944 c         1112 g         1327 t
ORIGIN      
        1 tatcaaataa gctgagatga aacgaaagtc tcttattctt aatctgttct taataactct
       61 aatatgcctg ccgctttcct tttcttcctg cagcattttg tatcctaatt caagcaaaca
      121 agtaaaacca aaatcacata aaacctttta caatccaaaa agacataaaa atgcaaaaag
      181 gactaaattg gtaagaatga aaaactaatt tatttctaac ttattgttta gaaactactt
      241 aattattatt taactactta atcttatgag aaaattactt atcctttccg ctctctttat
      301 tccaattatc tctgtttgcc agcaacttgt ttccttgaat gttgataaaa ttatgcggga
      361 tccaaaatgg atgggaaatt ttcccggaac tccatactgg tctgaagatg gaacaacgct
      421 gttttttaat tggaatcctg aaaataatcc cgatgattcc ctgtattcat ttaagcccgg
      481 agtttccaat gctccggtaa aagtaggtct ggtggatcgt gtgaatcttc cttcaaggtt
      541 tggcagttac aaccgtgcga gaacgaaaaa aatctatcaa aaagataatt ccctcatcct
      601 gctggatatt ccctcaaaaa agaaaatcgt aataatcgaa ggtatgggtc gagtgtccaa
      661 tgcatcgttc aatcatgcgg aaacaaaggt tttatttacc atggagaaca acctgattag
      721 ttgggagatt gctacaggta cgtttaagca attgacagat ttcagaaccg gaaatgaaag
      781 aaaagagacc aagacttccg atcaggatat atggctggga gatgatcaac tggcttggct
      841 tagggttttg aaagagagaa aagagaaccg ggaaaagtca agagaaatga ataatcttga
      901 agaacctgta tcaccaaaaa caatgtacct ggggcagtcc tccattcgca atatacagtt
      961 gtctccggat gaaaaatata tcacatacat gctttcggct tccgcgaatt ccaagggtac
     1021 cgaagttcct acctatgtta cggagtctgg ttatgctgaa atgcttcggg cacggcctaa
     1081 agttggcgcg gaaggctcca atgttgaaat gaagatttat gacattgccg gcgatacaac
     1141 gtataaagtg aacgtggaaa ccttgccagg cattgaagat attccagact actattcaga
     1201 ctatccaaaa tacaaagaca agaaacctga aaagagaaaa gtacttcccg gaagcctgaa
     1261 ctggtctccg gacggaaaat atgccttctt tgaaatacga agtcaagata ataaggatcg
     1321 atggattgga actcttgatg ttaaaaccgg aaagataaat gtgctcgatc gacagcgtga
     1381 tgaagcctgg attgcaggcc ctggcattgg atggtcagga tttccaggcg gaaatttagg
     1441 atggatgccc gatagtaaac gaatctggtt ccaatcagag gagtccggct attcacatct
     1501 ttacaccatt gatatcacca ataataagaa aacagcattg acaaaaggga attatgaaat
     1561 ttatgatccg gtaatatcag cggataagaa gagttggtac ttcgaagcaa atgttgacca
     1621 tccgggtgat gtgcaagttt ataaaatgcc aatcaatggg ggcaagatgg aaaagttaac
     1681 caacaaaccc ggccgcaatg atttctacct ctctccggac aattcaaaaa ttggtatatc
     1741 gtattcctac tccaatcaac caaccgagtt gtattggatg ccggctggcg cgaatataat
     1801 gaaccagatt acaaactctc aatctgaaga atataagtcg tacccatggc gcgacccgga
     1861 aatagtgact tttaaagccc gcgatggtgc tgaagtgtat gcaaggcttt acagaccggc
     1921 cattccacag caaaacggac ccgcggtaat ttttgttcat ggcgccggtt atcttcaaaa
     1981 tgcccataaa tggtggagca gttactttcg cgagtatatg tttcataatc tgctggccga
     2041 caacggatat actgttttgg acattgatta caggggaagt gcaggctatg ggagtaattg
     2101 gagaaccggt atttacagac acatgggtgg caaggattta attgatcagg ttgacggcgc
     2161 aaaattccta actgaacagt accaggttga tgcgaataag atcggaattt acggtggttc
     2221 atacggcggc ttcattacgc ttatggcgat gttcacggag ccgggagtgt ttgcggccgg
     2281 tgccgcgcta agatctgtag cggactgggc tcactataat catccctata cagctaatat
     2341 tttgaatacc ccggtcgaag atagcctggc ctacgcccgt agttcgccaa tctattttgc
     2401 tgaaggcctt gaaggaaaat tactgatgtg tcatggtatg gtggacacta atgtgcattt
     2461 tcaggatgtt gttcgattgg ctcagcgatt aattgagctg ggcaaagacg attgggagct
     2521 tgcagtttac ccattggaaa atcatggttt tgttgagccc agcagctgga cggatgagta
     2581 cacccgtatt ttcaagctct ttgaagataa tctgaaatga ccaatctgac catcgttttg
     2641 cttttgagtt gccgcacgcc taaattgtca ggcttaataa ttcatccatg aagaaaattt
     2701 tgatacttgc aggcatttgc ctttttgttt cctgccaacc catgcaggaa acaattgaaa
     2761 gtaaacaggg actcattgct aaaaatggaa tagtagtttc cgcccaccca ctcgcctcag
     2821 atgttggaaa atcaatttta caaaaagggg gaaatgcaat cgatgctgca attgcggttc
     2881 aatttgcact cgctgttgtt tatccggtgg caggaaatat tggcggcggt ggattcatgg
     2941 taataaggaa gaatgacggt tccacctatg cgctcgatta tagagaaaaa gctcccgggg
     3001 caggaagtcg tgacatgtat atcggagaga atggcgaagt aatccagggc ttaagtctta
     3061 acggagccct cgcttcaggc attcccggtt ccgtggatgg catggcgaaa gcttttgaaa
     3121 agtttggcac actgaagtgg agtgaattag tgcaaccggc tattgatttt gcagaaaatg
     3181 gctatgagct tacagaacgt gaagcccgtg gcctgaatcg taatgcagaa agttttgaga
     3241 agcatagtac aattcgtcct gacttcatac tgaaaaatga atgggccgcc ggtgaaaaga
     3301 tattaatgaa ggaccttgct gaaactttga tgagaattcg ggattatgga cgagatggat
     3361 tctataaagg tgaaaccgcg caattgatcg ttgaagaaat ggaaagaggt aatggtataa
     3421 ttacccttga agacctggcc aactatgaag cacaatggcg agatccgata gttggaaact
     3481 ataaaaacta caaaatgatc ggtatgcctc cgccctcttc aggtggagtt gctcttcttc
     3541 aattgcttgg aatggttgag ggcttccctc tctctgaatg ggggtggaat tccgcgaaaa
     3601 cggtgcacct tctggttgag gctgaaagaa gagtgtatgc agacagagct gcgcatcttg
     3661 gagatccgga ttactatgca gtgccggtaa gcaatctgat tgataataat tacctggact
     3721 caaggatgca aaactttaat gctgagctgg caactccaag tgattctata aaggcaggca
     3781 gttttcaagt ttatgaaagc acagaaacaa cccatttttc cattatcgat tctgatggca
     3841 atgcggtttc ggtaaccaca acgctcaacg gtggatatgg gtccaatgtc atcgttggca
     3901 atgcaggatt tctcttaaat aatgagatgg atgactttag cattcagccg ggtttcccga
     3961 acatgtatgg cctgattggg ggcgaagcta acgcgattga acccgggaaa agaatgctta
     4021 gctccatgac accaactatt gttgaaaaag accgccaatt gtttatggtt gtcggtactc
     4081 cgggtggttc tacgattata acgagtgttt ttcaaacaat tttgaatgta attgaatttg
     4141 gaatgggaat gcaggaagcc gtatcctcag gaagattcca tcaccaatgg cggccggaaa
     4201 atgtttttat ggaatctaat gcaatcactt ctgaagatag tgctatgctg gtaaagatgg
     4261 gtcataaatt cctgggttca ggcggaatag gacgtgttga cgcgattctg gttttgggtg
     4321 aagaaggact ggaaggtggt gctgaccccc ggggcgatga taaggcctcc ggttactaat
     4381 ttctaacaat tataaattca acccgccggt taaactttcg ggtttcttca cttgcattgg
     4441 aagcaatggg ctgagttcct ccataaccac ggcctgaaat tctaacggca gaaattccat
     4501 tcaatattaa atattcttta accttgtcta ctctgtcctg gctcaatttt aaatttaatt
     4561 tagagtttcc ctggttatct gtgtgccctg acaattcaat ctccatgttt ggattgtcaa
     4621 tcattagttg tgctatgatc tgcaattgtt tgtaactgga ttcaattaaa tctggcgttc
     4681 cccgataaaa atttacattg tcaagattta ctaccgagcc taattccaca ggataaaggg
     4741 aaacggaaag tgcaagcaaa tcctcttcag gattcactct tatttcctcg ctgcgtgaaa
     4801 gataaccgct tgcgctaaaa accagttcat aaac
//
LOCUS       DASWHX010000020        16493 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_2229854, whole genome shotgun sequence.
ACCESSION   DASWHX010000020 DASWHX010000000
VERSION     DASWHX010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 16493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16493
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2229854"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            287..1195
                     /gene="sdaAA"
                     /locus_tag="VGA21_01095"
     CDS             287..1195
                     /gene="sdaAA"
                     /locus_tag="VGA21_01095"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015264444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HEX9649128.1"
                     /translation="MSVLFDDFKSWKSYCEEKNLSFFEPVLEYEERQKGRKAADTWNG
                     LSQAYQVMCDAINTGLKEDMTSRSGMVNNGGKKVFNNKLSVLSPEFQKLIARTLAAKE
                     VNSCMGRIVAAPTAGASGILPGVLYTLQEIHNLDNQKILEALLVAAGIGLVIEKRASL
                     AGAVGGCQAETGSAAAMASGAIVYCLGGNITQILNAVAITIQCTLGLICDPVAGLVEV
                     PCIVRNASAASIAYSSAQIALSDVDSVIPADECVEAMGEIGQSMESRYKETAMGGLAV
                     TSTGQKISKRVLIQDIEILPDQDNHP"
     gene            complement(1196..1840)
                     /gene="upp"
                     /locus_tag="VGA21_01100"
     CDS             complement(1196..1840)
                     /gene="upp"
                     /locus_tag="VGA21_01100"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009580594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEX9649129.1"
                     /translation="MVNILSETNSVFNNFISELRDKTIQTEKMRFRKNMERIGQLMAY
                     ELSKTMNYRMIEVETPLGTSEVQIMSDSIVLIPILRAALPLYNGLLSVFDNSESGFVG
                     AFREEGKEIKINFGYLAAPDITDKVLILIDPMLATGKSQIVTLENLERHGKPSHIHLV
                     SVVAAPEGIEFINSELAGRSFTIWTVAKDERLNDDKYIVPGLGDAGDLSFGPKL"
     gene            1915..2832
                     /locus_tag="VGA21_01105"
     CDS             1915..2832
                     /locus_tag="VGA21_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009196493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX9649130.1"
                     /translation="MELKLLKDLCSISAASGSEIHVRDFILKYLKKHSGSWKKKPKIL
                     YGDEFQDCLILLFGKPRTAVFAHMDTVGFTVRYENQLIPVGGPSAENGVALVGRDALG
                     EIQCKLKIDKQDRLYYDFGRAIERGTHLTFKPDFRETGKSVQCCSMDNRLGIYIALKV
                     CEQLENGIIVFSAWEEHGGGSVPFLVKYIYENYKIRNYLVSDVTWVTDGVHPGKGTVI
                     SLRDQNIPRKKYLNQIIEIAERAGINYQLEVESSGSSDGREIQTSPYPLDWCFIGPAE
                     KNVHSPDEKVNKKDILETIKLYKVLMDDL"
     gene            2882..3415
                     /gene="hpt"
                     /locus_tag="VGA21_01110"
     CDS             2882..3415
                     /gene="hpt"
                     /locus_tag="VGA21_01110"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017732794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEX9649131.1"
                     /translation="MKLHDLEFELYLKSDKIQEKVIELAKRINKDYRNISPLFVPILN
                     GSFMFAGDLLKLIKLECLVSFIKVSSYSELESSGNLKQLIGVNEKIFGRDVIIIEDII
                     DSGKTMTELLEKFLELGPNSLEVVSLFRKEKAKQYDMNIKYIGFEIPNDFIVGYGLDY
                     NGYGRNLKDIYKLKSDE"
     gene            3408..3995
                     /locus_tag="VGA21_01115"
     CDS             3408..3995
                     /locus_tag="VGA21_01115"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013454066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEX9649132.1"
                     /translation="MSKKLNLVLFGPPGAGKGTQSEKIIEKYKLTHLSTGDLFRKHLG
                     EGTDLGKQAQAYMDNGKLVPDEVVIEMVGEKLSNNNNASGYIFDGFPRTVAQAAALDK
                     LLSSLNDSITLTIALEVDEAELRKRLLERGKTSGRADDQNEEKINTRIKVYMDETLPV
                     ADYFDNQKKFYPVEGVGTIDEIFNRICQTIDAKIK"
     gene            4006..4998
                     /gene="obgE"
                     /locus_tag="VGA21_01120"
     CDS             4006..4998
                     /gene="obgE"
                     /locus_tag="VGA21_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017732797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEX9649133.1"
                     /translation="MASTNFIDQVKIFVKSGSGGAGSVHFRREKYVPKGGPDGGDGGR
                     GGHIILKGNAQLWTLLHLKYKKHIMAANGNPGEGKDKSGAEGKDEIIEVPLGTIARNN
                     EDGKILAEITTDSEEVILQEGGMGGLGNQHFKTSTNQAPRHAQPGIPGKESWITLELK
                     ILADVGLVGFPNAGKSTLLSVLSAAKPEIADYPFTTLTPNLGVVPYYDDKSFVMADIP
                     GIIEGAAKGKGLGTRFLRHIERNSILLFMIPCDAENICKEYEILLNELSTHNPELLDK
                     KRILALTKADLIDDELETEINRDLPQGIPAIFISAVTRKGIAELKDLIWKNLQE"
     gene            5085..5699
                     /locus_tag="VGA21_01125"
     CDS             5085..5699
                     /locus_tag="VGA21_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009580604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEX9649134.1"
                     /translation="MSEEKMEKNPSLDQKGNSETQVEDEKIQDKTPENLENAPEPENV
                     KTETSREEAEDESEKLSKELTDAKDKYLRLFSEFENFRRRTARERLDLIQTSTEGLMV
                     ELLPVLDDFGRAAASIENGSDKKSVAEGIQLIENKFKRALEQKGLKAMQVKQGDDFNP
                     ELHEAVSQIPAPKKKLVGKIIETVEDGYFLGEKVIRFAKVVIGG"
     gene            5705..6817
                     /gene="dnaJ"
                     /locus_tag="VGA21_01130"
     CDS             5705..6817
                     /gene="dnaJ"
                     /locus_tag="VGA21_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009053364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEX9649135.1"
                     /translation="MSKRDYYEVLGVERSASPEEIKKSYRKIALKFHPDKNPDDDSAT
                     DKFKEAAEAYEVLSNPEKKQRYDQFGHSGMNGGGFSGGGMSMDDIFSQFGDIFGGHNP
                     FESFFGGGGSRSRGRKGSNLRIKLKLTLEEMAKGVEKKIKVKRMLLADGVTFNPCSSC
                     GGQGQVRKVVNTMIGQMVSTSTCPACGGSGKIIKNKPAGSDQNGLTMAEEIIPIKIPG
                     GITDGMQLSMNGKGNDPPFGGIPGDLLILIEEIPHKVLTRDGNNLVYDLYISFADAAL
                     GTSVEIPTLDGKVKIKIEPGTQPGKILRLRGKGVKDIHGYGNGDQLIHVNVWTPKILS
                     PEERQILENLRNSPNFKPNPGKQERGFFERMKEFFN"
     gene            6891..7781
                     /locus_tag="VGA21_01135"
     CDS             6891..7781
                     /locus_tag="VGA21_01135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649136.1"
                     /translation="MRRYLILLSLGVLCADLGYSQVKTHFILPKEKTFDKVDLTFTLP
                     KGTCYVSPKPDSEPVSIASNQDIENYVHSFEKKIRDRTCVTNINLEGKSDGGYGESIS
                     YRIFKNSGDEGNQIWKIYLTENIPYKLDLNYGIGDAFIDLSGLSVSDLKIKTGSANLN
                     VGFISDVPNQIDMDAFNVKVDLGSVNFRKMYLSNAKSVIADVGFGNLYLDYSIAPKVK
                     SEIRASVGAGDLIVIVPADETPIIVNVKSSMLCRIKISGDFREIKENVYVNEGYHPDA
                     GNLMTFTVDVSMGNIIFKSR"
     gene            7890..8801
                     /locus_tag="VGA21_01140"
     CDS             7890..8801
                     /locus_tag="VGA21_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013410135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX9649137.1"
                     /translation="MSTILETRKINKSYANHQALKNLDIAVPAGSIFGLLGPNGAGKT
                     TLIRIINQIIAPDSGEIIVDGEPLKPSHIEIIGYLPEERGLYKKMKVGEQVLYFARLK
                     GLSRSDAMEKIKVWFTKWDIKDWWNKKVEDLSKGMAQKIQFIATVIHEPKLIILDEPF
                     SGFDPINANLIRDEILELREKGSTIIFSTHRMESVEEICDHVALINKAEKILDGPKSQ
                     IKEEFRTNTFILEHKGSIGLANGSYTIEASSDIEHGLIRSVLKLKSGTPNEMLRELIQ
                     QTEIHSFVEQIPSMNDIFITKVKSVNA"
     gene            8798..10120
                     /locus_tag="VGA21_01145"
     CDS             8798..10120
                     /locus_tag="VGA21_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017732801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9649138.1"
                     /translation="MNKTLLIIQREYLSRVQKKSFIIMTILTPVIFVGIYAVVILLTI
                     KPGEEKTINVVDESGLFMEKFSNTDAITYSYNNINVEAGKELVTSGEYSGLLVIPNID
                     LKKPEGITLYSQQALGISVERNIERTIKNEIEDIKLKASGIDKAILDSIKTDVNLKAL
                     NIGSGGGTGEEKESNSALASGIGIFTGFIIYLFLFVYGTQVMRGVVDEKASRIVEIVI
                     SSVKPIQMMMGKIIGIAAVGLSQFLIWIILSTFLWTAATALLMGDQTINSDNTEQLAE
                     QEVSGNANSRAEMVGQINSMIAKTNLPKILGLLIFYFVTGYLFYAALFAMIGSAIDTE
                     ADAQQFIFPIILPIIFSFMMYGAVINDPNGQIAFWLSMIPFTSPIIMMIRVPFEIPNW
                     QIFLSMAFMIGGFIGTTWLASRIYRVGILMHGSKVNYKTLAKWFMMKN"
     gene            10186..11385
                     /locus_tag="VGA21_01150"
     CDS             10186..11385
                     /locus_tag="VGA21_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017732803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9649139.1"
                     /translation="MANPLRDSDPLSFYKDKSKVKWIVLVASLLISIGSIYYTNYLVE
                     QLKETERRFINLFARSLEHTINEDANLTFLTQEIIAPNNSIPVIWADSQGEPFDYRNL
                     RIDSTGGNESIREQLIAELEIMKRIYDPIPIMISEDSEYQFIYYKNSFLLTQLIFYPY
                     IQLSVIAIFAFIAYLAFSYSRAAEQNQVWVGLAKETAHQLGTPISSLMAWVEHLRLEN
                     SDKNREIIDELDKDISRLQMITERFSSIGSVPVMKEENITQVVNQVGAYLRTRISSKV
                     NLEVSSLSDNIIAKINAPLFEWVIENLCKNAVDAMSGIGNLQIRIMRGSDYKVIIDIS
                     DNGKGIPKSAFSKVFHPGFTTKKRGWGLGLALSRRIIENYHNGRLFVKNSEPDKGTVF
                     RIELSTR"
     gene            11637..13142
                     /gene="atpD"
                     /locus_tag="VGA21_01155"
     CDS             11637..13142
                     /gene="atpD"
                     /locus_tag="VGA21_01155"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015265593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEX9649140.1"
                     /translation="MANIGRVTQIIGPVVDVSFDKEGKLPNILDALEVTRPSGQVVVL
                     ECQQHLGEDRVRTVAMDSTEGLIRGMDVVDTGRPIAVPVGEEIKGRLFNVVGNAIDGM
                     NAPKTKKTLPIHRPAPRFEDLSTTSEVLFTGIKVIDLLEPYAKGGKIGLFGGAGVGKT
                     VIIMELINNIAKAYSGMSVFAGVGERTREGNDLLREMIESDVINYGKEFKESMAKGEW
                     DLSKVDYKALQTSQATLVFGQMNEPPGARARVALSGLTIAEYFRDGDGEGQGKDILFF
                     MDNIFRFTQAGSEVSALLGRMPSAVGYQPTLATEMGAMQERITSTKRGSITSVQAVYV
                     PADDLTDPAPATTFSHLDATTVLSRKIAELGIYPAVDPLDSTSRILSAEVLGEEHYRT
                     AQHVKEILQRYKELQDIIAILGMDELSEEDKEVVHRARRVQRFLSQPFHVAEQFTGTP
                     GVLVDIKDTIKGFNMIMDGELDHLPEMAFNLVGTIEDAIAKGEKMVAEAEA"
     gene            13147..13401
                     /gene="atpC"
                     /locus_tag="VGA21_01160"
     CDS             13147..13401
                     /gene="atpC"
                     /locus_tag="VGA21_01160"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020531508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HEX9649141.1"
                     /translation="MYLEVITPEQKVFEGEVTITTLPGSDGSFQILNHHASLVSSLGK
                     GTLSFKTKEGDYLEHKIEGGVVEVLNNKVIVLAEKILKDD"
     gene            complement(13398..13709)
                     /locus_tag="VGA21_01165"
     CDS             complement(13398..13709)
                     /locus_tag="VGA21_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014771782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6787 family protein"
                     /protein_id="HEX9649142.1"
                     /translation="MSWIDKLKSRWNLKSTADVLIILLVFALTGTTVLVIKRPLLSSM
                     GEEVVNNVWFSVAYYILILPIYNVFLLFYGFLLGRFKFFWEFEKRFFSRIFGKKAERT
                     T"
     gene            complement(13711..14403)
                     /locus_tag="VGA21_01170"
     CDS             complement(13711..14403)
                     /locus_tag="VGA21_01170"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015813224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEX9649143.1"
                     /translation="MLPFQVLFEDNHLLIVNKSAGMLVQSDDTGDIPLIDHCKEYIKQ
                     KYEKPGAVFLEPVHRIDRPVSGIVIMARTSKSLERMNEIFRRKEIEKKYFAIVENQPR
                     EMSGRLIHWLIRDREKRKSFVYEKQAEGLLNAELHYEVLIELQGKYLLEIILQTGRPH
                     QIRAQLAAISSPIQGDLKYGAPKANSDGNISLHNRYVSFLHPVKKEKIEVKAPLLATV
                     EWQLFLTLAENR"
     gene            14543..16471
                     /gene="dnaK"
                     /locus_tag="VGA21_01175"
     CDS             14543..16471
                     /gene="dnaK"
                     /locus_tag="VGA21_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013453996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEX9649144.1"
                     /translation="MGKIIGIDLGTTNSCVAVMEGNEPVVIPNSEGRRTTPSVVAFLD
                     GGKGERKVGDPAKRQAITNPTNTISSVKRFMGKKYSEVSEERKGMSYTVEKGNNDTVR
                     MRIGDRLYTPQEISAMVLQKMKSTAEDYLGTEVTEAVITVPAYFNDAERQATKEAGQI
                     AGLDVKRIINEPTAASLAYGLDKKNKDMTIAVYDLGGGTFDISILELGDGVFEVKSTN
                     GDVHLGGDDFDQKIINWLAEEFKNDEGIDLRKDPMALQRLKEAAEKAKIELSSSTETE
                     INLPYIMPVDGVPKHLVKKLSRSKFEQLVDDLVQRTLDPCKKAMSDAGLSSKDIHEVI
                     LVGGSTRIPRIQEEVEKFFGKKPSKGVNPDEVVAVGAAIQGGVLTGEVKDVLLLDVTP
                     LSLGIETLGGVFTKLIESNSTIPTKKSEVFSTAADSQPSVEIHVLQGERSMAKDNRTI
                     GRFHLDGIPPAPRGVPQIEVTFDIDANGIINVSAKDKGTSKQQEIRIEASSGLTDEEI
                     EKMRKEAEANAETDKKEKEKADKLNQADSMIFQTEKQLKDYGDKLSEGNKSSINSALA
                     ELKKAHESKDLASIESALANLNKAWEAASQEMYKATEAQQQQDTPGAGAEGPTPGESG
                     ENVSDVEYEEVDDKKDKQ"
BASE COUNT         5289 a         3142 c         3625 g         4437 t
ORIGIN      
        1 gtgttcccat tattaagatt gttttccatg tcagtgacaa ttaatcgatg aagtcaagtt
       61 ctgaggttaa ttattttttg agtttctaca agttattata tagcttttat aatttatcat
      121 actataatat agtcttattc aaattattta atgaattaat atacattgat ctactggact
      181 cccttgtttg aatcataaag aattgcaaac tgtgttcgaa tggacttatt aaaagctttt
      241 gagtttgaac tttcatacct ttggcaaagc aataattaac ttaacgatgt cggtattatt
      301 tgacgatttc aaaagctgga aatcctactg tgaagaaaag aatctttcat tttttgagcc
      361 tgtattagaa tatgaagaaa gacaaaaagg aagaaaagcg gcggacacat ggaatggatt
      421 atcacaagct taccaggtaa tgtgcgatgc aattaatacg ggtcttaagg aagatatgac
      481 ctcccgttca ggcatggtga ataacggcgg aaagaaagtc tttaacaata aactttctgt
      541 gcttagccct gaattccaaa aactgattgc gagaaccctg gcggcaaagg aagtaaacag
      601 ctgtatggga agaatagtag cggcacctac ggcgggtgct tccggaatcc tgccaggtgt
      661 actttacacc ctgcaggaga ttcacaatct tgataatcaa aagatactgg aagccttgct
      721 tgttgcagcc gggatcggac ttgtgattga aaagagagct tcacttgcag gtgccgttgg
      781 cggttgccag gcagaaaccg gaagtgcagc tgcaatggct tccggagcga ttgtgtattg
      841 ccttggtggc aatattaccc aaattctgaa cgctgtggca ataactattc aatgcacgtt
      901 gggattaata tgcgacccgg tggcaggact cgtagaagta ccctgcatag taagaaatgc
      961 cagtgcagca tccattgctt attcttccgc tcaaatcgct ctttcagatg tcgatagtgt
     1021 gattccggcc gatgaatgcg tggaggctat gggtgagatt ggccaaagta tggagtcgcg
     1081 atataaagag acagcgatgg gtggcttagc ggtaacatcc accggacaaa aaatttcaaa
     1141 acgcgtctta attcaggata ttgaaatatt gcccgatcag gataaccatc catgattata
     1201 atttaggtcc aaaacttaag tcgcccgcat ctccaagccc cggaacaata tatttatcat
     1261 cattcaatct ttcatctttt gcaactgtcc agatggtaaa agaccggcct gcaagttcag
     1321 aatttatgaa ttcaatccct tccggagccg ccacaacaga aacaagatga atatgagaag
     1381 gttttccgtg ccgttccagg ttttcaaggg ttacaatttg ggatttaccg gttgccagca
     1441 taggatcgat caaaatcagg actttatccg ttatatccgg cgctgccaaa tagccaaaat
     1501 ttattttaat ttctttccct tcttccctga acgccccaac aaatcctgat tccgaattat
     1561 caaaaacact gagcaatcca ttatacaaag gaagcgccgc ccgaagaatt ggtatcaaca
     1621 caatcgaatc tgacatgatt tgcacttcgg aagttcccaa tggagtttct acctcaatca
     1681 tcctatagtt catagttttt gataactcat aagccatcag ttgtccgatg cgttccatat
     1741 tctttctgaa gcgcattttc tcagtttgga tagtcttatc tctcaactcg cttatgaaat
     1801 tgttgaacac agagttcgtt tctgaaagta tattgaccat tttaaaatgt tttcaataaa
     1861 attaagtttt taattatgaa aatatgaatc agctacagaa ttttgaggtc cattatggaa
     1921 ctaaaattac tgaaagatct ttgttcaatt tcggcagctt cgggatctga gattcacgtc
     1981 cgggatttta tcttgaaata cctgaaaaaa cattccggtt cctggaagaa aaagcccaaa
     2041 attttgtacg gtgatgaatt tcaggattgc cttatcctat tgttcgggaa acctcgcact
     2101 gctgtttttg cacatatgga tacagtgggt ttcactgtcc gctatgagaa tcaactgatt
     2161 cccgtgggtg ggccgtccgc tgaaaatgga gtggctctgg tggggaggga tgccttgggc
     2221 gaaattcaat gcaaattgaa gattgacaaa caagacaggc tttattacga tttcggaaga
     2281 gctattgagc gtggaactca cctcaccttt aaacccgact ttcgggaaac cggtaaaagt
     2341 gttcaatgtt gttccatgga taatcggctg ggaatctata tagcactgaa agtttgtgaa
     2401 caacttgaga acggcattat tgtattcagt gcatgggaag aacatggtgg cgggtccgtc
     2461 ccatttctgg ttaagtatat ctacgaaaat tataaaatca gaaattatct cgtcagcgat
     2521 gtaacctggg ttacagacgg cgtgcatccg ggtaagggaa cagtaatttc attgcgtgat
     2581 caaaatatac cgagaaaaaa atacctgaat caaataatcg aaattgcaga aagggctggg
     2641 attaattatc aattggaagt tgaaagttcc ggaagcagcg acggccgcga aatacaaacc
     2701 tccccttacc cgctcgattg gtgctttata ggtcctgcag agaaaaatgt ccactcaccg
     2761 gatgaaaagg taaacaagaa agatattctt gaaactatca aactgtacaa ggtattgatg
     2821 gatgatttat agcttttaaa tttattggct atatttgccg ccccaacctt aattaaaccc
     2881 aatgaagctg catgatcttg agttcgagtt atacttgaaa tcagataaaa ttcaggaaaa
     2941 agtaattgaa ttggccaaaa gaataaacaa ggattacagg aatatttcgc cactgtttgt
     3001 tccaattctt aatggttctt ttatgttcgc cggagacttg ctgaaactta ttaaactgga
     3061 atgcctggta tctttcataa aagtgtcttc ttattcagaa cttgaatcat caggaaatct
     3121 gaaacaactg attggcgtta atgagaaaat atttggccgg gatgtaataa tcattgaaga
     3181 cattattgat tcaggaaaaa ccatgacgga gttgttggaa aagtttttgg aactgggccc
     3241 taattctttg gaagttgttt ccctattccg aaaagaaaaa gcaaagcaat atgatatgaa
     3301 cataaagtat attggatttg aaatcccaaa tgattttatt gttggctacg gtttagatta
     3361 taatggctat ggcagaaact tgaaagacat ttacaaacta aaaagcgatg agtaaaaaac
     3421 taaacttggt tcttttcgga cctcctggtg caggcaaggg gacacaaagt gaaaaaatta
     3481 ttgagaaata taaacttacc catttgtcta ccggcgattt gttcagaaaa catcttgggg
     3541 aaggaacaga tttaggaaaa caagcccagg cctatatgga taatgggaaa ttggttcctg
     3601 acgaagtagt gattgaaatg gtaggtgaaa aactttcaaa taacaataat gccagtgggt
     3661 acatttttga tggttttccc agaaccgttg cacaggctgc cgcactagac aagctgctta
     3721 gcagtctgaa cgattctatt actcttacta ttgctttgga ggttgacgaa gcggaattaa
     3781 ggaaaagatt gctggagcgg ggaaagacca gtggacgtgc cgatgatcag aatgaagaaa
     3841 aaatcaacac ccgtataaaa gtatatatgg acgaaaccct gcccgttgca gattattttg
     3901 ataatcagaa gaagttctat cctgttgagg gtgtgggaac catcgatgaa atcttcaacc
     3961 gcatttgtca aaccatagac gctaaaataa agtgatccta ttccgatggc ctccacaaac
     4021 tttattgatc aggttaagat attcgtaaag tccggatcgg gaggggccgg ttccgtacat
     4081 ttccggaggg aaaaatatgt tccaaaaggc ggccctgatg gtggcgatgg tggccgtggt
     4141 ggtcatatca ttctaaaggg aaatgctcaa ctttggactt tacttcacct gaaatacaag
     4201 aaacatatta tggctgccaa tggcaaccca ggagagggta aggacaaatc aggagctgag
     4261 ggaaaagatg aaataattga agtaccgctt ggaacgatag caaggaacaa cgaagacgga
     4321 aaaattcttg ctgaaattac tacggatagt gaagaggtaa tcctgcagga aggtggaatg
     4381 ggtgggctcg gcaaccagca tttcaagacg tcaactaatc aggcaccgag gcatgctcaa
     4441 cctggcatcc ccggtaaaga atcatggatc actttggaac tgaaaatact cgcggatgtg
     4501 ggattggttg gatttcctaa tgcaggaaaa tcaaccttac tttccgtact ttcagcggca
     4561 aaacccgaaa ttgctgatta tccattcact acacttactc ctaatctcgg agtagtaccc
     4621 tactatgatg ataaatcgtt tgttatggca gatatacccg gcatcattga aggcgctgct
     4681 aagggcaagg ggcttggtac aaggtttctg aggcacattg aaagaaattc cattttactc
     4741 tttatgatac catgcgacgc ggaaaacatc tgtaaagaat atgaaatatt gctcaatgaa
     4801 ttgagtacgc acaaccctga acttctggat aaaaaaagaa ttcttgccct aacaaaagca
     4861 gatttgattg acgatgaact tgaaaccgaa attaatcgtg atttacctca agggattccg
     4921 gcaatcttta tttcagcggt cacacggaag ggaatcgcag aattaaagga cttaatctgg
     4981 aaaaaccttc aagaatgatt tatgccaatc tgtcatagaa atgtaaattg gcaaagaaat
     5041 tgactctaaa acgtaaattt ccacctattt tgaatattta aataatgagc gaagaaaaga
     5101 tggagaaaaa tcctagtctg gatcaaaaag gaaattctga aacgcaggtt gaagatgaga
     5161 aaattcagga taaaactcct gagaatttag agaatgctcc agagccggaa aatgtgaaaa
     5221 cggaaaccag ccgggaagaa gctgaggatg aatctgaaaa gcttagtaaa gaactgactg
     5281 atgcgaaaga taaatacctg cggttatttt ctgaatttga aaatttcaga agaagaactg
     5341 ccagggaaag gcttgatttg attcaaactt caaccgaagg attaatggta gaattacttc
     5401 ctgttcttga tgattttgga agagccgcgg catccattga aaatgggagt gataagaaat
     5461 ctgtggctga aggaattcag ctgattgaaa ataaatttaa gcgcgccctg gaacaaaaag
     5521 gtctcaaagc aatgcaggta aaacagggag atgactttaa tcctgaattg catgaagcag
     5581 tttcacaaat acctgcacct aagaaaaaac ttgtaggtaa aataatcgag acagttgaag
     5641 acggatattt tctgggcgaa aaagtaatcc gatttgctaa agttgtgatc ggaggataaa
     5701 ttaaatgagc aagagagatt attacgaagt acttggagtt gaaagatcag ctagtcctga
     5761 ggaaataaaa aaatcctaca ggaagattgc attgaaattt catcccgata aaaacccgga
     5821 cgacgactca gcaacggata agttcaaaga ggcagccgaa gcctacgaag ttttgagcaa
     5881 tcctgagaaa aagcaacgtt atgatcaatt cggacattcg ggaatgaatg gcggcggttt
     5941 tagcggtggc ggcatgagca tggatgatat cttctctcaa tttggagata tcttcggcgg
     6001 ccacaatccg ttcgaaagtt tctttggcgg tggtggaagc cggtcacggg gcaggaaagg
     6061 ttccaatctg cgaataaaac tcaagcttac cctggaagaa atggcgaagg gtgttgagaa
     6121 gaaaatcaag gtgaagcgga tgttgcttgc tgatggagtt actttcaatc cctgtagttc
     6181 atgcggggga caaggtcagg taagaaaagt ggttaacacc atgattggtc agatggtgtc
     6241 aacctccacc tgtcctgctt gcggaggaag tggaaagata atcaagaata agccggccgg
     6301 ctcagatcaa aatggactca ctatggccga agaaattatt ccaattaaaa tacctggcgg
     6361 cattaccgat ggcatgcagt tatccatgaa cggaaaggga aatgacccgc catttggtgg
     6421 aattccgggt gaccttttga ttttgattga agaaattccc cataaggttc ttacccgtga
     6481 cggcaacaat cttgtgtatg acctctatat aagttttgca gatgcggccc ttggaacatc
     6541 cgtggaaatt ccaaccctgg atggtaaggt gaaaataaag attgaaccag gtactcaacc
     6601 cggcaaaatc ctgagattgc gtgggaaagg cgtaaaagat attcatggct acggaaatgg
     6661 cgaccaacta attcatgtaa atgtgtggac tcccaaaatc ctttcacccg aagaaagaca
     6721 aattctggaa aacctgcgga actcaccaaa ctttaaacct aatcctggca agcaagagcg
     6781 gggatttttt gaaagaatga aagaattctt taattaagcc tctggcaatc agtattctgg
     6841 catttctagc attgctgtaa cctaagcgta atttttgcgt taaaattggc atgcggcgat
     6901 acttaatttt gttaagtctg ggagtgcttt gcgcggattt gggctattca caagtcaaaa
     6961 cacactttat tcttccaaaa gaaaaaacct ttgataaggt agacctcact tttactctgc
     7021 ccaaaggaac ctgttatgtt tctccaaagc ccgactccga acctgtaagt attgcgagta
     7081 atcaggatat tgaaaactat gttcatagtt ttgaaaagaa aatcagagac agaacctgcg
     7141 ttacaaacat caacctggag ggaaagtctg atggtggcta tggggaaagc atttcttacc
     7201 ggatattcaa aaattcagga gatgaaggca atcagatttg gaaaatctat ctcaccgaaa
     7261 acatccctta caaacttgat ctgaattatg gaataggaga tgcatttatt gatttgtcag
     7321 gactttccgt tagcgatttg aaaatcaaaa caggtagcgc aaatcttaac gtgggattca
     7381 tatccgatgt ccctaatcag atcgatatgg atgcctttaa tgtaaaggtt gatttaggtt
     7441 ctgttaattt ccggaaaatg tacctctcta atgccaaatc ggtaattgca gatgttggat
     7501 ttggaaatct ctatcttgat tatagcatcg cccccaaagt taaaagtgaa atcagggcca
     7561 gcgtcggtgc cggtgattta attgtaatag ttcctgcaga cgagaccccc atcatagtta
     7621 atgtgaagag ttccatgctt tgtcgcatca aaattagtgg agatttcagg gagataaagg
     7681 agaatgtata tgtgaatgaa ggttatcatc ctgatgccgg caatctgatg acattcaccg
     7741 tggatgtatc tatgggtaat attattttta aatcccggta atttaggtcc tctcacctgt
     7801 catctatcca aatcaaagtc cattcatcat ttaattacta atcctttgga gtaattgcta
     7861 gatttgcaat tgaaaaatcc atttcaatca tgtcaacaat ccttgagacg cgaaaaatca
     7921 acaaaagcta cgccaatcat caagccctca aaaacctcga tattgccgtg cccgccggat
     7981 ccatatttgg tcttttaggc ccaaacggtg ccggaaaaac taccttaatt agaattatca
     8041 atcaaatcat tgcaccggat agcggtgaaa taattgtcga cggcgaacca ttgaaacctt
     8101 cacacatcga aatcatcgga tatcttcctg aggaacgcgg tctttataag aaaatgaaag
     8161 taggagaaca agttctctat tttgccaggc tgaagggctt aagcagatca gatgcaatgg
     8221 aaaaaattaa ggtatggttt acaaaatggg atattaaaga ttggtggaat aaaaaagttg
     8281 aagatttaag taagggtatg gcgcaaaaga ttcaattcat tgctacagta atccatgaac
     8341 ccaaattgat tattctggac gaaccctttt caggtttcga tcccatcaat gctaatttaa
     8401 taagagatga aatcctggaa ctgagagaaa aaggcagcac aattattttc tccacccatc
     8461 ggatggagtc cgttgaggaa atttgtgatc acgtagccct gattaataag gcggaaaaaa
     8521 ttctggatgg gcctaaaagt caaattaaag aagagtttag aaccaacaca ttcatattgg
     8581 aacacaaggg aagcattggg ttggcaaatg gtagttatac tatagaagct tcatcggata
     8641 tcgagcatgg attaatccga tcagttttga aattgaagtc aggcactccc aatgaaatgt
     8701 tgagggaact tattcaacaa acagaaattc atagtttcgt tgagcaaatt ccttcaatga
     8761 acgacatctt cattaccaag gttaaatcag taaacgcatg aacaagacac tactaattat
     8821 ccaaagagaa tacctgtcaa gggttcagaa gaaatcattc atcataatga caattctgac
     8881 ccctgttata ttcgtaggaa tctatgcggt tgttatttta cttaccataa aacctggtga
     8941 agaaaaaaca atcaacgttg ttgatgaaag tggcctgttc atggagaagt tttccaacac
     9001 cgatgcgatt acttattcgt acaacaacat taatgttgaa gccggaaaag aactggtaac
     9061 ttcgggcgag tattcaggac tcctggttat cccgaacatt gacctgaaaa aacccgaggg
     9121 gatcaccctt tattcacagc aagcgctggg aatatcagta gaaagaaata ttgaacgcac
     9181 cattaaaaat gaaatcgaag atattaagct gaaagcgtca ggaattgaca aagctatatt
     9241 ggactccatt aagacagatg ttaatcttaa agcgctaaac attggttcag gtggaggtac
     9301 aggtgaagaa aaagaaagta actccgcgct ggcatcaggc attggtattt ttactgggtt
     9361 tattatttat ctttttcttt ttgtgtatgg aacgcaggtg atgcggggag tggttgatga
     9421 aaaagccagc aggatagttg aaatagttat atcatcggta aagcctattc aaatgatgat
     9481 ggggaagata ataggaattg ccgcggttgg attgagccaa ttcctgattt ggataattct
     9541 atcaactttc ctttggacag ctgctacagc attattaatg ggagaccaaa caatcaattc
     9601 agacaataca gagcaacttg cagaacaaga agtttccggt aatgcaaatt cccgggctga
     9661 aatggttggc caaattaatt ccatgatcgc caaaaccaac ctgccaaaaa tattaggcct
     9721 gctaatcttt tattttgtta caggatactt gttttacgcg gcattgtttg caatgatagg
     9781 ttccgcaatt gacacagagg cagatgctca gcaatttatc tttcctataa ttttgccaat
     9841 tatattttct ttcatgatgt atggggctgt aataaatgat cccaacggac aaatagcctt
     9901 ttggctctcc atgattccat tcacttcacc aatcataatg atgattcgag taccttttga
     9961 aattccgaac tggcaaattt tcctttccat ggcttttatg attggcggtt ttattggcac
    10021 cacctggctg gccagccgca tctacagagt cgggattttg atgcatggct ccaaagtgaa
    10081 ttataaaaca cttgcaaagt ggtttatgat gaaaaactaa ttctaaccat cataaaaact
    10141 ggcattatgg agttactttt gctatgttgg ctgtaaagtc gcgccatggc aaatcccctg
    10201 cgagattccg atcctttaag tttctataaa gataagtcca aagttaaatg gattgttctc
    10261 gtcgcatccc tgctcatcag catcggatct atttactata ccaactatct cgtggagcaa
    10321 cttaaggaaa cggaacgaag attcataaat ctctttgcca ggagcctgga gcatacaata
    10381 aatgaagatg cgaacctgac gttccttacc caggaaatca tcgctcctaa taattcaatt
    10441 ccggttatct gggctgattc ccaaggcgaa ccctttgatt accgtaattt aagaattgac
    10501 agtaccggag gaaatgagag tataagagaa caacttatag cagaattgga aattatgaag
    10561 cgaatttatg atccgattcc aattatgatt tcagaagaca gcgaatatca gtttatctat
    10621 tacaagaatt cgttcctgct aacccaatta attttttacc catacattca actaagtgtt
    10681 attgccatct tcgccttcat tgcatacctg gctttcagtt attcaagagc agcggaacaa
    10741 aaccaggtgt gggtggggct tgccaaggaa actgctcacc agcttggaac accgatttcg
    10801 tcattaatgg catgggtaga acatcttcga ctggaaaaca gtgataagaa tcgggaaatt
    10861 attgatgaat tggacaaaga tatctccagg cttcaaatga tcaccgagcg attctcaagc
    10921 attggctccg taccggtaat gaaagaggaa aatataaccc aggtagtgaa tcaggtcgga
    10981 gcttacctga ggacgcgaat ctcatcaaaa gtgaacctgg aagtttcaag cctatcagat
    11041 aatatcatag caaaaattaa tgcaccttta tttgaatggg tgattgagaa tctttgtaag
    11101 aatgccgttg atgctatgtc aggcattggt aatcttcaaa ttcgaataat gcgcggcagc
    11161 gattataaag taattataga tataagcgac aatggaaagg gtatcccaaa atccgccttt
    11221 agcaaagtct ttcatcccgg gttcacaacc aaaaaacgag gctgggggct tggccttgct
    11281 ttaagtcgca gaatcattga aaattaccac aatggacgcc tttttgttaa gaattcggag
    11341 cccgataagg gaacagtttt tagaattgaa ttgagtacca gataacaaat tctagccact
    11401 ccaaaaatcc ccaggtaatg tctccgtgga gtcttttacg tgcgccccac tttgcagaca
    11461 ttgaaaatca atcaattttc acttaaaata aaaagtggaa gtgtggcaag cgctgatgtt
    11521 aatttcattt ggatttaacg gagacttacc aaagacccaa tttggtttaa gcaattttag
    11581 tcttactttt gcagtcgaat ttttaccatt cattcctaaa ataataagaa aaatacatgg
    11641 caaatattgg tagagtaacc cagataattg gccccgttgt ggatgttagt tttgataaag
    11701 agggcaaact tcccaacatt ttggatgcat tggaagtaac gcgaccaagt ggacaggtcg
    11761 tggttttgga atgccagcag cacctcggcg aagatagagt gagaactgtt gccatggata
    11821 gcacagaagg cctgatcagg ggaatggatg ttgtcgatac gggcagacca attgcagttc
    11881 ccgttggtga agaaattaaa ggcagattgt ttaatgttgt tgggaatgcc atagatggaa
    11941 tgaatgctcc aaaaacaaag aaaacgctgc caattcacag acccgctccc agatttgaag
    12001 atctttccac cacttcagaa gttttgttta ccggaataaa ggttattgac ttgctggagc
    12061 cttatgctaa aggcggaaaa attgggctct ttggaggggc aggtgtgggt aagacggtta
    12121 tcatcatgga gttaatcaat aacattgcga aagcctattc cggcatgtca gtgtttgcag
    12181 gagttggtga aagaacaaga gaaggtaatg acctactacg cgaaatgatt gagtcagacg
    12241 taattaacta cggaaaagaa tttaaagaat cgatggcgaa aggagaatgg gatctttcaa
    12301 aagtagatta caaagcactt caaacttcac aagctaccct tgtatttggc caaatgaatg
    12361 aacctccggg agcaagagcc cgggtcgctc tttcgggact gacaattgct gaatatttca
    12421 gggacggtga tggcgaaggc cagggaaaag atattttgtt ctttatggat aacatcttca
    12481 ggtttacaca ggcaggttct gaagtatctg cgctactggg caggatgccc tctgcggttg
    12541 gttatcaacc tactttggcc acggagatgg gtgccatgca agagcggatt acctctacca
    12601 agcgagggtc tatcacttct gtacaggcag tatatgtacc cgccgatgac cttaccgacc
    12661 cggcacctgc cacaacattc tcacacctgg atgcaacaac cgtgctatca cgaaagattg
    12721 ccgagttggg aatctatccc gctgtggatc cgctggattc aacatccaga attctgagcg
    12781 ctgaggtatt gggcgaagaa cattaccgca cagcacagca cgtaaaagaa attctgcaac
    12841 gatacaaaga acttcaagac atcattgcca tccttggcat ggatgagttg tccgaagaag
    12901 acaaagaagt tgtgcacaga gccagaagag ttcaacgttt cctctcacaa ccattccatg
    12961 ttgcggagca atttaccggt actcccggcg tattggtaga tattaaagat accatcaaag
    13021 gttttaacat gatcatggat ggcgaattgg atcacctgcc tgaaatggca tttaacctgg
    13081 ttggaaccat tgaggatgca attgctaaag gagagaaaat ggtagccgaa gctgaagctt
    13141 aatcaaatgt atctggaagt aataacacct gaacaaaaag tatttgaagg cgaagtcacc
    13201 atcactacgc tgcccggaag cgatggctct tttcagattt tgaatcacca tgcttcatta
    13261 gtaagttccc ttggaaaagg aacacttagc tttaaaacaa aagagggcga ttatctggaa
    13321 cacaaaatag agggcggtgt cgtagaagtt ctgaacaata aagtaattgt actggcggaa
    13381 aaaattttaa aggacgatta agttgtcctt tcagccttct ttccaaaaat cctgctgaaa
    13441 aatcgttttt caaattccca aaagaactta aaacgtccta ataaaaaacc atagaataaa
    13501 aggaaaacgt tataaatggg aagtatcaaa atatagtagg ccacggaaaa ccagacatta
    13561 ttgacaactt cctcacccat actgctgagt aaaggtcttt tgatcaccaa aaccgtagtt
    13621 ccggtaagtg caaaaaccag cagaatgatc aaaacatcag ccgtcgattt caggttccac
    13681 ctggatttca gtttgtctat ccaactcatg ctatcgattt tccgccaaag taaggaacaa
    13741 ttgccattct acggttgcta ataacggagc ttttacttct atcttttctt ttttcacagg
    13801 atgaagaaaa gaaacatagc gattgtgcag gctgatgtta ccatcggagt tcgcctttgg
    13861 tgcaccatac ttcaaatcac cctggatagg actacttatt gcagccagct gcgctctaat
    13921 ctgatgtgga cggcccgttt gcaaaattat ttccaataaa tattttcctt gtaattctat
    13981 cagcacttca taatggagtt cggcatttag caagccttct gcttgctttt catacacaaa
    14041 cgattttcgc ttttccctgt ctctgataag ccaatgaatt aatcgaccgg acatctccct
    14101 gggttgattt tccactatgg cgaaatattt tttctcaatt tctttacgac ggaatatctc
    14161 attcattctt tccaaacttt tggatgtccg agccattatg actattcctg acacaggtct
    14221 gtcaatccta tgcaccggct caagaaatac agctcctggt ttttcatact tctgctttat
    14281 atattctttg caatgatcaa tcaatggaat atctccggtg tcatcagact gcaccagcat
    14341 ccctgctgat ttattaacaa taagtaaatg attatcctca aacagaacct gaaagggaag
    14401 cattcaatac acttaatttt cctccaaatt cggctttttt ctgcaaatag tgggttttct
    14461 gccattatgg cacaaatacc tcattggcat aggcattgtt aatgactatt tactaaatga
    14521 aaaatttaag atcattcgaa tcatgggaaa aataattgga attgacttag ggacaaccaa
    14581 ctcatgcgta gccgtaatgg agggaaatga accggtggtg atccccaata gcgaaggcag
    14641 gcgaacgact ccttcggttg ttgcattttt agacggcgga aagggagaac gaaaagttgg
    14701 agatcctgcc aaaagacagg caataacaaa tccaaccaat acaataagtt ctgtaaagag
    14761 atttatgggt aagaaatatt ctgaagtatc cgaggaaaga aaaggaatgt cttacaccgt
    14821 tgaaaaagga aataatgaca ccgtacgaat gcgtataggt gatcgacttt atacacccca
    14881 ggagatttct gccatggtgt tgcagaaaat gaaatccacc gcggaagatt atctgggaac
    14941 cgaagttact gaggccgtta tcaccgtccc cgcttatttt aatgatgccg aaagacaggc
    15001 aacaaaagaa gccggacaga tcgcaggact cgatgtgaag agaattatca acgaaccaac
    15061 ggccgcatct ctggcctacg gtcttgataa gaagaataaa gacatgacaa ttgccgtgta
    15121 tgaccttggc ggcggtactt tcgatatctc aattctggaa ttaggtgatg gggtatttga
    15181 agttaaatct accaatggtg atgtccacct gggcggcgat gacttcgacc agaaaatcat
    15241 aaactggtta gccgaagaat tcaagaatga tgaaggaatt gatttaagaa aagatcctat
    15301 ggctttacag cgattgaaag aagctgctga aaaagccaag attgaacttt caagttccac
    15361 agaaacggaa atcaatcttc cttacattat gcccgtcgat ggagttccta aacacctggt
    15421 gaagaaactc tcaagatcaa agtttgagca gttggtggat gatttggtac aaaggactct
    15481 tgatccttgc aaaaaagcca tgagtgatgc tggcctttcg tcaaaagata ttcacgaagt
    15541 gatattggtc ggtggttcaa cccgtatccc caggatacag gaagaagtgg agaaattctt
    15601 cggtaagaaa ccttccaaag gtgtaaatcc tgatgaagtt gtcgcagttg gtgccgctat
    15661 tcagggtggt gtacttaccg gtgaagtgaa ggatgttttg ttgctggatg taacgcccct
    15721 ttccctgggt attgaaaccc ttggcggagt gttcaccaaa ctaatagaat ctaattcaac
    15781 tatacccacc aagaaatcag aagtgttctc caccgcggca gatagccagc cttctgtaga
    15841 gattcacgtt ttgcaaggcg agcgatccat ggcaaaggat aacagaacta ttggcagatt
    15901 tcacctggat ggtattccgc cggcaccgag aggagttcct caaatcgaag tgacattcga
    15961 tattgatgcc aacggtatca ttaacgtttc cgcgaaagat aaaggaacta gcaagcagca
    16021 ggagattagg attgaagcct cgtcaggatt aaccgatgaa gaaatagaaa aaatgcggaa
    16081 agaagctgag gccaacgcgg aaaccgacaa gaaagaaaaa gaaaaagcag ataaattaaa
    16141 tcaggctgac agtatgattt tccaaactga aaagcagttg aaggattatg gcgacaagct
    16201 tagcgaaggt aataagtcct ccatcaattc tgcgctggcc gagttgaaga aagcacacga
    16261 gagcaaagac ctggcaagca ttgaaagtgc cctggctaac cttaacaaag cttgggaagc
    16321 agcttcacaa gaaatgtaca aggctaccga agctcaacaa cagcaagata ctccgggggc
    16381 aggcgctgaa ggaccaacac ctggtgaatc aggtgagaat gtgtctgatg ttgaatacga
    16441 agaagtagac gacaaaaagg ataagcaata acgtgcacgt ataattaata tat
//
LOCUS       DASWHX010000021         4151 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_1861802, whole genome shotgun sequence.
ACCESSION   DASWHX010000021 DASWHX010000000
VERSION     DASWHX010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 4151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4151
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1861802"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..301)
                     /locus_tag="VGA21_01180"
     CDS             complement(<1..301)
                     /locus_tag="VGA21_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015813619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649145.1"
                     /translation="MNLLLQSELFLLILDSIGIEYAMKRILWSCLVLIPLAVTGQVMK
                     PILWEHSVVPADPNPGDEIELVFKATIDPKWYLYSSDFDPEVGPMVTTFEFESSDD"
     gene            complement(370..807)
                     /locus_tag="VGA21_01185"
     CDS             complement(370..807)
                     /locus_tag="VGA21_01185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649146.1"
                     /translation="MQPELSLTIRFGGVVEKSLFFVSGAPQVNLYDEGDSLVIQFSGI
                     RNNQAEIIKFHIEGIQINALLNLTTLDNTKIILNGIDNFGAIENLNTLCNSSGGIGQL
                     YLRRITVTDENSIIISGTFGFTINGSSCGNIDVSYGRFDYVVR"
     gene            complement(909..1553)
                     /locus_tag="VGA21_01190"
     CDS             complement(909..1553)
                     /locus_tag="VGA21_01190"
                     /inference="COORDINATES: protein motif:HMM:NF024957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEX9649147.1"
                     /translation="MNSPLRYAHLPKTFLILAIFILLSNNCWSQIFILGGFNRNYIKQ
                     DYLAGNVEGVSNYHFGAAWSMRPVKNLSYLSIERQFLFLWKGYKQSVGTTDYEAELLY
                     LSFPFIIKYSYRFVSLQTGISLDLLTFTSDVKYAESSKFNNFDSSLIIGFDLFEQKRL
                     GFYARFCIGLTQILDYYSFDKLGNFTGEIQDIKNRNFLLGLKLRLSNHIFPHEK"
     gene            complement(1666..3042)
                     /locus_tag="VGA21_01195"
     CDS             complement(1666..3042)
                     /locus_tag="VGA21_01195"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013454724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEX9649148.1"
                     /translation="MVEKQVKEYLDSGKQRFLDELLELLRIPSVSADSKHKADVARAA
                     EYVKLRLEEAGAENVEVCKTPGHPIVLAQKFVSESVPTVLVYGHYDVQPPDPLDLWDS
                     PPFEPVIKNEKIYARGSADDKGQFFMHIKAFEALNKTGNLNCNIKFMIEGEEEVGSKH
                     LAQFVKDNKQKLSCDVILISDTSIISNSSPSITVGLRGLSYMEVELTGPNRDLHSGVY
                     GGAVANPINTLCKMIATLKDEKNRVTIPGFYDDVADLSAQERAAMAKRPFNLDEYKSD
                     LGIDEVEGEEGYSTIERVGIRPTLDVNGIWGGYTNEGAKTVLPSKAHAKISMRLVPNQ
                     NSDKITDIFAKHMEEITPPTMKIKVTAHHGGEPTVTPTDSKAYRAASKAFEEAWGKTP
                     IPSREGGSIPIVALFKEVLGTDTVLLGFGLDSDAIHSPNEHYGLFNFYKGIETITLFY
                     KHFQNSKI"
     gene            complement(3051..3704)
                     /gene="plsY"
                     /locus_tag="VGA21_01200"
     CDS             complement(3051..3704)
                     /gene="plsY"
                     /locus_tag="VGA21_01200"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020597421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEX9649149.1"
                     /translation="MEILLLAAGILIAYLLGAVPTAVWFGKLYHGIDVREHGSGNPGA
                     TNTFRVLGKRAGTIVLLIDVVKGLMAASLPVVLLKLQTFPENELVNFKIIFGLMAVVG
                     HLFPVFLSFKGGKGVATLMGMMLAIHYEVAILSAVIFMILLFLTKYVSLSSIIGALSF
                     PFMLLLIPRFQGGEPMLIIFGFILFVVILVTHNRNIHRMFKGEENKTHLFNRKNNQN"
     gene            complement(3756..>4151)
                     /locus_tag="VGA21_01205"
     CDS             complement(3756..>4151)
                     /locus_tag="VGA21_01205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649150.1"
                     /translation="GVSNILREDINGSFEGFLEIIKRLDGERIDLFLKASVDSWYYFS
                     YENNKLITYSSNPAYNEFIATKSNAGKSKIGEFVFIPGDESEILTFVNRFRNDYLNIQ
                     QPYQLNAAPIEEDLTDDGFGGKKKKDGNR"
BASE COUNT         1256 a          853 c          787 g         1255 t
ORIGIN      
        1 aatcgtcaga agattcgaac tcgaaggtgg taaccattgg gccgacttcc ggatcaaaat
       61 cgctggagta taaataccat ttgggatcta ttgtagcttt aaagaccagt tcgatttcat
      121 cgccagggtt tgggtccgcc ggaacaacag aatgttccca cagaattggt ttcataacct
      181 gaccggtaac tgccagggga atcaatacga gacaactcca caaaattctt ttcatcgcat
      241 attctattcc aatagaatca agaattaaca aaaatagttc actctgaaga agaagattca
      301 tactttgaac aataattatt aagatttttt gatgatggta attttggtac ggaattccta
      361 tttgctaaat caccgcacca cgtaatcaaa ccggccgtac gaaacatcga tatttccgca
      421 ggaagaacca tttatggtaa aaccaaaagt tcctgagatt attatggaat tctcatctgt
      481 aactgtgatt ctgcgcaggt ataattgacc aatccctccg gaagaattgc ataaggtatt
      541 tagattttca atggctccga aattgtcaat tccgttaagt ataatttttg tattgtcaag
      601 agtcgtcaga ttgagcaatg cattgatttg aataccttca atatgaaatt taatgatttc
      661 ggcctggtta tttctaattc cagaaaactg aataaccagg ctgtcgcctt catcataaag
      721 atttacctgg ggagccccgg atacaaagaa aagagatttt tccactacac ctccaaatct
      781 gatcgttaat gatagctccg gctgcattgt tgccagtttc ggtatacatt ggaagccgcg
      841 gatctattgg gtcaggaata aaaatcccat cgcaggaaat aagcagaagt aacaatacaa
      901 gaatgtctct atttttcatg agggaaaata tggttactga gtcttaattt tagccctaac
      961 agaaaattgc gattttttat atcttgaatt tcgccggtaa aatttcccaa tttgtcgaat
     1021 gaataataat caagaatttg agttagtccg atacaaaacc gggcataaaa acccagccgt
     1081 ttttgttcga acaaatcaaa tccaataatc aaggatgaat caaaattatt gaatttggat
     1141 gattcagcat atttaacgtc tgaagtgaaa gtaaggagat cgagggatat acccgtttgc
     1201 aatgaaacaa aacggtatga atatttgatg atgaaaggaa atgaaaggta aagaagctcc
     1261 gcttcataat ctgtagtacc taccgattgt ttatagcctt tccaaagaaa taaaaactgc
     1321 ctttctattg aaagatagga aagattttta acaggtctca tgctccatgc cgcgccaaaa
     1381 tgataattag aaacaccttc aacatttccc gccagataat cttgctttat ataattccgg
     1441 ttgaagcctc ccagtataaa tatttgcgac caacaattat tcgagagcag aataaaaatt
     1501 gcaagaatca aaaaagtttt tggcaggtga gcataacgaa ggggagaatt catatgattg
     1561 aaatttctta tcaatttgga caatacggtt attctttaat aagcagagaa taatgagtaa
     1621 ggatttaaag aagtgactcc gattaaaatt ccagccgaac cggcatcaaa ttttagaatt
     1681 ctgaaaatgt ttgtaaaaga gagtaatggt ctcaattcct ttatagaaat tgaataatcc
     1741 atagtgctca ttcggggaat gaatggcatc agaatccagg ccaaaaccca gtaaaaccgt
     1801 atctgttccc agcacttcct tgaaaagcgc gacaataggg atgctgcctc cttcgcggga
     1861 aggaattggt gttttgcccc acgcttcttc aaaagctttt gaagctgccc ggtaggcttt
     1921 tgaatctgtt ggagtaaccg ttggctcgcc accatgatgt gcagttacct tgattttcat
     1981 tgtgggcgga gtaatttcct ccatatgttt ggcgaaaata tccgttattt tatcactgtt
     2041 ttgattggga accagtcgca tggaaatttt tgcatgggct tttgaaggta atacagtttt
     2101 tgctccctca tttgtgtaac caccccaaat gccatttaca tccagtgttg gcctgatacc
     2161 cactctttca atggtagaat atccttcctc tccttccacc tcatctatcc ccaaatctga
     2221 tttgtattca tccagattga aaggtctttt agccatggca gctcgttctt gagctgagag
     2281 gtcagcaaca tcatcataaa atcccgggat cgtgacacgg tttttttcat cttttaatgt
     2341 ggcgatcatt ttacaaaggg tattgatcgg atttgcaaca gctccgccat acactcctga
     2401 gtgcaaatct ctgtttggac cggttaactc cacttccata taacttaacc cacgcagtcc
     2461 tactgtaatt gatggtgacg agtttgaaat tatgctcgta tctgaaatga ggataacatc
     2521 gcaggataat ttttgcttat tgtcctttac aaattgcgcc aggtgtttcg atcccacctc
     2581 ttcttcccct tcaatcataa acttgatatt gcaattcaga tttccggttt tgttaagggc
     2641 ttcgaaagct ttgatatgca tgaaaaactg ccctttgtca tcggcagaac ctctggcata
     2701 gattttttca tttttgatta caggttcaaa gggtggtgaa tcccaaaggt caagaggatc
     2761 aggcggctga acatcgtagt gtccatacac cagcactgta ggtacggatt ctgatacaaa
     2821 tttctgtgcc aggacaatgg gatgtcctgg tgttttgcaa acctccacat tttctgcgcc
     2881 tgcttcttca aggcgcagct ttacgtattc ggcagccctg gctacatccg ctttatgctt
     2941 gctgtcggca ctaactgaag gaatcctcaa taattccaat aattcatcaa ggaatctttg
     3001 tttacctgaa tccagatatt ctttaacctg tttttccacc atatatatat ttaattctga
     3061 ttattttttc tgttgaagag gtgcgtttta ttttcttcgc ccttaaacat tctatgaata
     3121 ttcctgttgt gcgtgactag tattactacg aagagaataa atccaaagat gattaacatc
     3181 ggctcgcctc cctggaaccg tggaatcagc aaaagcataa atgggaacga taacgcgcca
     3241 attattgaag aaagagatac gtattttgtc aaaaataaca aaatcataaa aatcacggcg
     3301 ctcaaaatgg caacttcata gtgtattgcc aacatcatcc ccattaaggt tgccactcct
     3361 ttgccccctt taaaactgag aaacaccggg aaaagatgtc ctacaactgc catcaacccg
     3421 aagataattt tgaaatttac taattcgttt tccgggaatg tttgcaattt gagcaggaca
     3481 accggaagtg atgcagccat taatccttta accacatcaa ttagcagaac aattgttccg
     3541 gctcttttgc ccaaaacgcg aaatgtatta gtggcacccg ggttgccact cccgtgttcc
     3601 cgaacatcaa tgccatgata taatttgccg aaccaaaccg cggttggtac agcgcctaat
     3661 aaataagcga tcaaaattcc ggcagcaagc aaaaggattt ccattgttta tgttcttggg
     3721 gccccaaata tacgatattg gtttaagaga gaaaattacc gattgccgtc ttttttcttt
     3781 tttcctccaa aaccatcgtc agtcagatct tcttcaatcg gggccgcatt taattggtag
     3841 ggctgctgga tattcaggta atcatttcga aaacggttta caaaggtgag tatttccgat
     3901 tcatctccgg ggataaacac aaattctcca atcttagatt tgcctgcatt tgatttggtc
     3961 gcgataaact cattgtatgc aggattggaa gagtatgtta tcagcttatt attctcataa
     4021 ctgaaataat accaggagtc aaccgatgct ttcagaaata aatcaatgcg ctcaccgtcc
     4081 aggcgtttaa taatttccag aaatccttca aacgaaccat ttatatcttc tcttagaata
     4141 tttgacactc c
//
LOCUS       DASWHX010000022         1536 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_3338683, whole genome shotgun sequence.
ACCESSION   DASWHX010000022 DASWHX010000000
VERSION     DASWHX010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3338683"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1536
                     /locus_tag="VGA21_01210"
     CDS             <1..>1536
                     /locus_tag="VGA21_01210"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9649151.1"
                     /translation="LRKQLSYSIINIGGLAIGIACSFVILLYAMQELSYEQKFDKHDR
                     IYRIATHFMTMGDFANGPEQAAKVLPDEYPWVEEITTAKVMGELPLFNKEIESVGIGL
                     KVRNNFFRIFNYPFISGSPEHALDNSGSIIITSKLSEKLFGGENPMGKIVEAGKEKNP
                     YVVTGVVDISDLNSHINADFWTAETPAEEEPNSNWLSASSYIYLLSSHNVSFGEIQEA
                     MDKLIEKNVYPRLGGDFSFDEWYARDDAFRLIVQPVDNIYLSGILNFDLSPGGNETAV
                     YVLLAIAILILIIASVNFINLTTARADKRSKEIGIRKVIGGSKLSLMSQFMMESILIS
                     LLSVILALGVAELILMMFQSFTGTILFNSVFENPTNIFLALGIGALLGILSGFYPALY
                     LAAFQPIHAIKKGTHPGKSNQSFRNILVVFQFTLSIALITSTLIIFGQLEFMKKKDLG
                     FNRSDVLILDNAYVLGNSFEDLKNKLENLDGVINISAVIRLPGSDNSFSITSLSVPGS
                     EESL"
BASE COUNT          462 a          277 c          319 g          478 t
ORIGIN      
        1 ctaagaaaac aattgagtta ttccataatc aatatcggcg gactggcaat tggaatcgcg
       61 tgcagttttg tgatactgct ttatgctatg caagagctaa gctatgagca gaaatttgat
      121 aagcatgata gaatttatcg aattgcaaca cattttatga cgatgggcga ttttgctaac
      181 ggcccggaac aagcggcaaa agtactgcct gatgagtatc cctgggtgga ggagattaca
      241 acagcaaaag ttatgggtga attgccatta ttcaataaag aaatagaatc agttggaatc
      301 gggctaaaag tgagaaacaa tttcttcagg attttcaatt acccttttat ctccggaagt
      361 ccggaacacg ctctggataa ttccgggtca ataattatta catcgaaatt atcagaaaaa
      421 ttatttggcg gcgaaaatcc tatggggaaa attgtagagg ctggcaaaga aaaaaatccc
      481 tatgttgtta ccggagtagt tgacatttct gatctcaatt cacatattaa cgcggacttt
      541 tggacggctg aaactccggc tgaggaggaa ccaaattcca attggctttc agcatcctcc
      601 tatatttatc tattgtcttc ccataatgtt tcattcgggg aaattcagga agcaatggat
      661 aagctcatag aaaaaaatgt ttatccaaga ctcggtggag atttttcatt tgatgaatgg
      721 tatgcaaggg atgacgcctt tcgtttgatt gttcagccgg ttgataatat ttatttatcc
      781 ggaattttga attttgactt aagccccggg ggaaatgaaa cagctgttta cgttcttctt
      841 gccattgcga ttttaatctt aataatcgct tcggtcaatt tcataaatct caccacggca
      901 agagctgata aacgatcaaa ggaaattgga attcgaaaag taatcggcgg ctcaaaatta
      961 tcattgatgt cacaatttat gatggagtca attttgatca gccttttatc tgtaatatta
     1021 gcgttaggcg tggccgaatt aattttgatg atgttccaat ctttcaccgg cactatttta
     1081 ttcaattcag tttttgaaaa tccaacaaat atttttctgg cacttggaat tggtgcttta
     1141 cttgggattt tgagcggatt ctatcctgcc ttataccttg cagcattcca gccgatacac
     1201 gcgattaaga aaggtactca tccgggtaaa tcaaatcaaa gtttcagaaa tattctggtt
     1261 gtctttcagt tcaccctttc cattgccctc attacaagca cgctcataat ttttggccag
     1321 cttgaattta tgaagaaaaa agatttaggc tttaacagat cggatgtcct gattcttgac
     1381 aacgcctatg tattaggtaa cagttttgaa gatcttaaga ataaactgga gaatcttgac
     1441 ggagtgatca acatttctgc agtaatccgg ctgcccggat ctgataacag ttttagcata
     1501 acgagcctta gtgtaccggg atctgaggaa tcattg
//
LOCUS       DASWHX010000023        10627 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_13418, whole genome shotgun sequence.
ACCESSION   DASWHX010000023 DASWHX010000000
VERSION     DASWHX010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 10627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10627
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13418"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(86..838)
                     /locus_tag="VGA21_01215"
     CDS             complement(86..838)
                     /locus_tag="VGA21_01215"
                     /inference="COORDINATES: protein motif:HMM:NF037167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEX9649152.1"
                     /translation="MNFEIRKCKLEEILHLRSQFLQENDFQIRYNACHERHWSDSYLI
                     SLDNQGVGYGSVKGKEELTDRDSIFEFYLHAHLRKNASALFEKLISDSGAKFLECQTN
                     DFFLTSLVFEFSQKIYSDTILFEDYIATGFKISDIEFRKRKDNEVTFPHRFDGEPGDY
                     VLIRNGIAVATGGFLLHYNIPYADLYMEVNPEFKNQGFGSYMLQEVKKECYLAGRVPA
                     ARCNIKNKISRSTLIKAGMKVCGFMLIGDLNS"
     gene            complement(870..1280)
                     /locus_tag="VGA21_01220"
     CDS             complement(870..1280)
                     /locus_tag="VGA21_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010610679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family peroxiredoxin"
                     /protein_id="HEX9649153.1"
                     /translation="MKRNATAVWIGTGKEGTGTMTTQSTVLNGAQYSYKSRFEQGIGT
                     NPEELVAAAHAGCFCMKLSFLIDGAGFKAERLESKCVVTLEDGSITTSHITLNAKIEG
                     IDKAKFDEVVAEAEKTCPISKSINAKISVDATLN"
     gene            1446..2609
                     /locus_tag="VGA21_01225"
     CDS             1446..2609
                     /locus_tag="VGA21_01225"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9649154.1"
                     /translation="MKTLPKNVWILTIAQSLVMAVSSLVVFVGGIVGAGLAPSEKLAT
                     LPVAAMIVGTAISVLPVTGLMQRLGRRNSFSFILAFSISNALATAYALHFESFYFFCF
                     NALLFGVTNACVMQFRFAAMESVSVELIPNAASAVLIGGIAAAFVGPEIALAGRNLYE
                     TEFAGSFFLLAGVFLVAFVVLRFFRNVPIKKSEMDEGPRPLKEISSQRVFWVALTSAA
                     TGYMIMSFIMTATPVSMNIIDHHSLSSTKWVIQSHIIAMYAPSVITGFLIRKVGISGM
                     MISGLVIYVLCIAIAFAGHDLINYWVSLIFLGLGWNFLFIGGTSLLPLSYRPAERFRV
                     QALNDFVVFGSQAIASLSAGWLVFAIGWESMLIVVIPIIVLQGGIVLRSAGQP"
     gene            3382..4491
                     /locus_tag="VGA21_01230"
     CDS             3382..4491
                     /locus_tag="VGA21_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009579952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9649155.1"
                     /translation="MTARFFGWRGNIFNQYLISLILIFLTSVICYFSPGFIDYRVVAL
                     ILMLVLSLLAMLFDIFPVLMAAILSAIIWDYLFIPPTFTFYIGTPEDALLFLMYFVIA
                     LINIVMTSRIRSFEKKARDKGEREKTIRLYNTLLNSLSHELRTPISTIVGAIDTIKEN
                     EHKLSERNKLELFNEIEIAGFRLNRHVENLLNMSRLEAGVLKPKLDWCDVNELIYTVI
                     KNNKNNASQRIIDFEPNEKLPFFKIDRGLTEHVLNNILHNALQYTPDKSIIVISVSLV
                     GDYCRFTISDNGEGVPPEELKSVFDKFFRLPQTGTGGTGLGLSIAKGFTEAQNGTIKL
                     ENIRSGGAKFTIEIPAEVSSLNDDLKNEQSVDTDN"
     gene            4451..5143
                     /locus_tag="VGA21_01235"
     CDS             4451..5143
                     /locus_tag="VGA21_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009579951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX9649156.1"
                     /translation="MTLKMNKALILIIDDEPQIRKLLKIILESNNYEVVQAAAGKEGL
                     LKAANHPPELILLDIGLPDKSGHEILKELREWYNKPIIILSVQDNESDIVNALDVGAT
                     DYITKPFRTGELLARIRSAIRSSQNINLDETIITCGDLKIDLIARTVKKNDEIVKFTS
                     TEYNLLALFAKNEEKVLTHQFILKEVWGIGYQTETQYLRVFVAQLRKKIEENPNNPRH
                     IVTESGVGYRFS"
     gene            5235..5561
                     /locus_tag="VGA21_01240"
     CDS             5235..5561
                     /locus_tag="VGA21_01240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649157.1"
                     /translation="MEKNGQSQAAKAAEILSIYFSTKKLIEELPSREISGISDYLNCY
                     QLLKARFDKSLITELDQCILETKKMIFLDINMNSEKEVHPRISRKLKRILENLLLLKE
                     RVQDSE"
     gene            5697..5834
                     /locus_tag="VGA21_01245"
     CDS             5697..5834
                     /locus_tag="VGA21_01245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649158.1"
                     /translation="MKKKENHLTPYEKWIKEIVHLKWPVVILFILALVLVILIDFFLT
                     D"
     gene            5909..7618
                     /locus_tag="VGA21_01250"
     CDS             5909..7618
                     /locus_tag="VGA21_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019598559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HEX9649159.1"
                     /translation="MKRIPFWVSLLAVLAVIFDLGFDQSAVIQKDLLRLYRISILVGI
                     VSTIFRYYFVEDRPRYKVWPFDALLVILLTGVMIGWNLPLLEKIQWLYTAVFAVFVRE
                     FSALKFDYSRHILNPAQLFIISFLSIILTGTLLLILPNSTHDGITPIDAFFTSTSAVC
                     VTGLIVVDTGSYFTKFGQVVIILLIQAGGLGIMTFTSYFSYFFKGGSSYENQLLLKDI
                     TMAEKMADVFTTLKRIILVTFIIETIGGVLIYTSLDTNTIPPVGERIFFSIFHAVSGF
                     CNAGFSTLPNSLYEPGFRFNYPLHLVIATLFIIGGIGFPILFNFSKYLKYKVVKQILR
                     IREQKTDIYLSRIININSRIVIITTIVLITLGTILFYFFEYDNTLAEHHGFGKVVTAF
                     FGAVTPRTAGFNSVDTSVLNFWTIMFVFLLMWIGASPASTGGGIKTSTFSIGILNFIS
                     LARGKDRIEYFGREIAEVSVRRAFAIISLSLIVIGAAVFLISSFDSDKDLILIAFECF
                     SAYSTVGLSLGITSELSSASKLVIIITMFIGRVSMLTILIALLRKVRYMKYRYPTEEI
                     LIN"
     gene            7632..8213
                     /locus_tag="VGA21_01255"
     CDS             7632..8213
                     /locus_tag="VGA21_01255"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEX9649160.1"
                     /translation="MKCIIIGLGNFGSSLSEKLTQMGHEVIGVDSKMSRVEALKEKIT
                     HTVCLDCTDSQAVSHLPLKDTDIVIVCIGEDEGDNIMATALMKRLKVKRLISRAVSPL
                     HETVLEAMGVDEIVHPEEETADRWAKKLNISGVIDSFDLTGEYSIIEATVPPRYVGKT
                     LLDAGFRKNYDVVVLTTIKVTEEDNLIGITRKI"
     gene            8351..9403
                     /locus_tag="VGA21_01260"
     CDS             8351..9403
                     /locus_tag="VGA21_01260"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HEX9649161.1"
                     /translation="MYRIIRPVLFIILSAIIYFSLLILFYHFEFEVPGSGVRNFVDVI
                     WWSVATIATVGYGDIVPVTTEGRTIGYVFLFLSVTVYAYVIGKVSTSIKSHYNNKKLG
                     FLGTNFTNHTVIIGWDDFGKKVVDQLIKANQKVAIVTEERNYVDQIYHLYSSKAVFVL
                     FASCTNYKYFNKLNIAECNIVFLNNGTDTDNLVHLLNLKRHFPGLEYIVNVDNPDLKN
                     NFYREGVKYVISGDELASKLMASYIFEPDVAFFNEEIITFADSDDKYDVKQFKVLDRN
                     PFLNREYSKVFFDLKREYRAILVGISREMNGKYMLKKNPADGTIILAGDYLLVIANRV
                     VAEKLSKLFGVKEGVN"
     gene            9602..9931
                     /locus_tag="VGA21_01265"
     CDS             9602..9931
                     /locus_tag="VGA21_01265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649162.1"
                     /translation="MNHIELKESIIAEYLSTGLSLRKLGIKYGLNFRNIHRWVMQYQG
                     RMKKPTTSKRVKPNPDLAQEGMPTDVKKLQTELRKARLLNEVLTEVINIAEEELGLPI
                     RKKYGTK"
     gene            9943..>10627
                     /locus_tag="VGA21_01270"
     CDS             9943..>10627
                     /locus_tag="VGA21_01270"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEX9649163.1"
                     /translation="MQRAPSRSVGMLCSLLGYSRQAYYQHIKVQEQEALKEDLLLQEA
                     LRIRKTQKRIGTRKLLIMMIDFMQQHTISIGRDAFFEMLRNQGLLVRRRKRSKPQTTF
                     SNHWLRKYQNLIIGFVPNAPNQLWVSDITYIHLEEGFAYLSLITDAYSHKIVGFYLGE
                     DLSAKGCIMALRMALKNNPLRDNLIHHSDRGLQYCCHDYVELLEGNSIKISMTQNGDP
                     LENAMAERVN"
BASE COUNT         3151 a         1984 c         2242 g         3250 t
ORIGIN      
        1 gttttttaat tcgtgaattc tgattcaaat tgcttcagtt ttttgaattt gtaatgcaaa
       61 taaataagaa atagtttttg gtgattcagg aatttaaatc tccgataagc ataaatccac
      121 aaactttcat accggcttta ataagtgttg accgggaaat tttatttttg atattgcaac
      181 gggcggccgg tactctcccc gccagataac attctttttt tacttcctgc aacatataac
      241 tgccaaatcc ctggtttttg aattccgggt ttacttccat atataaatcc gcataaggaa
      301 tattataatg gaggagaaat cccccggtag ctacagcgat cccattgcgg ataaggacat
      361 aatcaccagg ctcgccatca aaccgatgcg gaaatgtaac ttcattgtct tttcttttac
      421 ggaattcaat gtcagaaatt ttaaaaccag tagcaatgta gtcttcaaac aaaattgtat
      481 cagaataaat tttttgagaa aattcaaaaa caagtgaagt aagaaaaaag tcattagttt
      541 ggcactcaag aaatttggct cctgagtctg aaataagttt ttcgaaaagc gcagaggcgt
      601 ttttgcgtaa gtgagcatgc aaataaaatt caaaaataga gtcccgatct gtcagctctt
      661 ccttcccctt taccgatcca tatcccacac cttgattatc aagacttatt aaatatgaat
      721 ccgaccaatg ccgttcatgg caggcattgt acctaatttg gaaatcattc tcttgcagaa
      781 attgtgaacg aaggtggagg atttcttcaa gcttacattt tcttatttca aaattcatgc
      841 aggaaaagag attggaaata atccaatccc tagtttaatg ttgcgtctac agaaattttg
      901 gcattaatgg atttggaaat cgggcaagtt ttctcagcct ctgctaccac ttcatcaaat
      961 ttggctttat caattccttc tatttttgca tttagcgtta tatgtgaggt ggtgattgag
     1021 ccatcctcca gggtaactac acatttggac tccagtcttt cagccttgaa tcctgcaccg
     1081 tcaatcaaaa atgaaagttt catacagaag catcctgcat gagcagcggc aaccaattct
     1141 tcagggttag taccaatacc ctgttcaaag cgggatttat atgaatattg tgctccattt
     1201 agtactgtac tctgtgttgt catagttccg gtaccctctt tcccggttcc aatccatacg
     1261 gctgttgcat ttcttttcat aattctattg tttgtttaaa gttgtttgaa atatttctta
     1321 atataataat ataggaataa caatcattga gcagtttggt tttttaagtc tttaaaataa
     1381 gatttattaa aatttttcaa tactgaaatc caaattcagg attaattgat attttgcggc
     1441 gctttatgaa gacacttccc aaaaacgtct ggatccttac aattgcccag tcgctggtta
     1501 tggcagtaag ttcactggtc gtttttgttg gtggaattgt gggagcaggc ctggcgccca
     1561 gtgaaaaatt agctactctt cccgttgcgg caatgattgt gggtaccgct atttcggtac
     1621 tgccggtaac cggcctgatg cagagattag gccgccggaa ttcattttcc tttatacttg
     1681 cgttcagcat tagtaatgct ctggcaaccg cttatgcttt gcattttgag tctttttact
     1741 tcttttgttt caacgcttta ttatttggtg ttaccaatgc ttgtgtgatg cagtttcgct
     1801 ttgccgccat ggaaagtgta agcgttgaac taataccaaa tgcagcttcc gcggttttga
     1861 ttggtggtat tgccgcggcc tttgtgggtc ctgaaattgc tttggcgggg agaaatctct
     1921 acgaaaccga attcgcaggc tcattttttt tgctggcggg cgtattttta gtggcatttg
     1981 ttgttcttcg attttttaga aatgtgccaa ttaaaaaatc agaaatggat gaagggccac
     2041 gaccattaaa ggaaatttca agccaacggg ttttttgggt agcattgacc agtgcggcaa
     2101 ccgggtacat gatcatgagt tttattatga ccgcgactcc agtcagcatg aatattatcg
     2161 atcatcattc cctttcttcc acaaaatggg tgattcaaag tcatataatt gcgatgtacg
     2221 ctccctcggt aataaccggc tttctcataa gaaaagtagg aatttccgga atgatgattt
     2281 ccggattggt aatctatgta ttgtgcattg cgatagcctt tgcaggacat gatttaatta
     2341 attattgggt ttcattgatc tttcttgggc ttggatggaa cttccttttt attggtggca
     2401 cctctttgct tccactgtcc tatcgacccg cggaacggtt ccgggttcag gcattgaatg
     2461 attttgtagt gttcggaagt caggctattg catccctatc agccgggtgg ttggtttttg
     2521 ctataggatg ggagagtatg ttgatcgtag ttataccaat catagttctg caaggtggta
     2581 tagttctgcg gtcggcaggc caaccttaaa ttaagggggg ggaagtaccc cctgcccgga
     2641 aattgtcccc ttgttttaag ggggactaca gggggttttg actaccattt tgagaaccac
     2701 ccctgcccct ccttaaaaag gaggggaaag tacccctgct cggaaataca gggggttttt
     2761 tccttcccac atagagcaaa tgcatttcta tattctgaac caatggaaat tgtccccctt
     2821 gttttaaggg ggacttcagg gggttttgac taccatttta agaaccacct tgcccggaaa
     2881 ttgtccccct tgttttaagg gggactacag ggggttttga ctaccatttt aagaaccacc
     2941 ctgcccggaa attgtccccc ttgttttaag ggggactaca gggggtttaa atacctgtta
     3001 gaaccacccc ctgcccgcaa attgtccccc ttgttttaag gaggactaca gggggtttaa
     3061 atactatttt cataaccacc cctgcccaac cttcggtcga caggccctcg ttaaaagaag
     3121 gagaggaata taagattaat taggtaattg gtgtactgtg cagacataag gaagggttag
     3181 aggttgcaca gaaatagata ttaggatcat gaaacggact tatgggatgt gcacccagcg
     3241 ctttgaatta aaacataaac cgttaggact gattttaagg attggcatgg atctccacac
     3301 cgtgcaagat ccgaacaacg tggagatctt tcagaatatt tttacaaaat atttataact
     3361 taattgatat ttttgagctg aatgacagca agatttttcg ggtggagagg caatattttc
     3421 aatcagtatc tgataagtct gatcctgatc tttttgactt cggtgatctg ttatttttcc
     3481 ccaggtttta ttgactacag ggtagtggct ctgatcttaa tgctggtttt gtctttactg
     3541 gctatgcttt ttgatatttt cccggtattg atggcggcta ttctcagtgc cattatttgg
     3601 gattacttat ttatcccgcc aaccttcact ttttatatcg ggacgcctga agatgcgcta
     3661 ctatttttga tgtattttgt tattgcctta ataaatattg tcatgacttc cagaatacgg
     3721 agtttcgaaa aaaaagcaag agataaaggt gagcgggaaa aaacgatcag gctttacaat
     3781 accttactta attctttatc tcacgaactg aggactccaa tctctactat tgtaggagcc
     3841 attgatacaa ttaaggaaaa tgaacacaaa ttatcagaac gaaataagct ggagttattt
     3901 aatgagattg aaatagcggg ctttcgtctt aaccgtcatg tagaaaatct cttgaatatg
     3961 agtcgtcttg aagcgggtgt tctcaaacca aaattagact ggtgtgatgt gaatgaattg
     4021 atttacactg ttattaaaaa caacaaaaat aacgcttcgc aacgtattat tgattttgaa
     4081 ccgaatgaaa aactgccctt cttcaaaatt gaccgcggat taacagaaca tgtactcaat
     4141 aacatcctgc acaacgctct tcaatatacg cctgacaaat caataattgt aattagtgtt
     4201 tcactcgtgg gtgattactg caggttcacg atctctgata atggcgaagg cgtgccacca
     4261 gaagaactga aatcggtatt tgataaattc ttcagacttc cccaaacagg taccggcgga
     4321 accggtttgg gattatctat agctaagggg tttacggaag cacagaatgg aaccattaaa
     4381 ctggagaaca taagatcggg cggtgccaaa tttactattg aaattcctgc agaagtttca
     4441 tctttaaacg atgaccttaa aaatgaacaa agcgttgata ctgataattg atgatgagcc
     4501 tcagatccgg aagctgctga agatcattct tgagagcaat aattatgaag tagtgcaggc
     4561 agctgcgggc aaggaaggcc ttttaaaggc ggccaatcac ccaccagaac ttatcttact
     4621 ggacattgga ttacccgaca aaagtggcca tgaaatactc aaagaactta gagaatggta
     4681 taataaaccc ataataattc tttcagttca ggataacgaa agcgacatcg ttaatgcact
     4741 cgatgtcggt gctacggatt acattacaaa gccattcagg acaggagaac tattggcacg
     4801 catccgttcg gctattcgaa gtagccagaa tatcaattta gatgaaacaa taattacctg
     4861 cggtgatctg aaaatagatc tgatcgcgcg aacggttaaa aagaatgatg aaatagtaaa
     4921 attcacttcc actgaatata atttactcgc cttatttgca aaaaacgagg agaaagtact
     4981 tactcaccag tttattctaa aggaggtttg gggtattggt taccagacag aaacgcagta
     5041 tttacgtgta tttgtagctc aactgcgaaa gaagattgaa gaaaacccaa acaaccccag
     5101 gcatattgtt accgaaagcg gagttggtta caggttcagc tagtctttat aaaatcttta
     5161 tagatacagg ccaaactttt attttctacc gatccattgg aagataaatt tgagttgcag
     5221 gtgacattct tatattggaa aagaacggac aatcgcaggc agcaaaagca gctgagatct
     5281 tatcaattta ttttagtaca aagaaattga ttgaagaatt accttccaga gagatttcag
     5341 ggatttcaga ttatctgaat tgctaccagc ttttgaaagc aaggtttgat aagtctttga
     5401 tcactgaact tgatcaatgc attctggaaa caaaaaaaat gatcttcctt gacattaata
     5461 tgaatagcga aaaggaagtt catcccagga tcagccgaaa gctcaagcgc attttagaaa
     5521 acctacttct tcttaaagaa cgagtacaag attcggagta attatgccaa ataatcaaaa
     5581 atcaaagtaa tgagttataa tgaagttctc cagattgcag tgataacact aagtattatt
     5641 ctgatcattt ggcttgttgg tgtagtgatc cgggagatct ataaaaaata gtttggatga
     5701 agaaaaagga aaaccatcta actccctatg aaaaatggat aaaggaaatc gtccacttga
     5761 aatggccggt ggtcatcttg tttattttgg cattagtact agtaattctt atcgactttt
     5821 tcctgactga ttaggatcgc tgactcatct tatgcgcata tcccggtggt ttcgatatcg
     5881 taataaaata aaggaaatcc tcaaatggtt gaaacgaatt cctttttggg taagcttgct
     5941 ggctgttctt gccgtaattt ttgatctcgg attcgaccag tcagcggtca ttcaaaagga
     6001 tcttctaagg ttatatcgca tttctatcct tgttggtatt gtctctacca tattcagata
     6061 ctattttgtt gaagaccggc cgcgttacaa agtatggcct tttgatgcgc tactggttat
     6121 ccttttaacc ggtgtaatga tcgggtggaa cttgccgctg ctcgaaaaaa ttcaatggct
     6181 ttacactgca gtatttgcag tttttgttcg ggagttttcg gccttaaagt ttgattacag
     6241 caggcatata cttaatcctg cacagctgtt tattatcagc ttcctctcga taatcctcac
     6301 aggaactctt ttgttgatcc tgcccaattc tacgcatgat ggcataactc ccatcgatgc
     6361 ctttttcaca tctaccagcg cagtttgtgt taccggctta attgtggttg acactggcag
     6421 ctactttaca aaatttggac aggtagtaat tatactactt attcaagctg gtggattagg
     6481 catcatgact tttaccagtt atttcagcta cttctttaaa ggaggatctt cgtatgagaa
     6541 tcagttgctg ctgaaagata ttacaatggc agagaaaatg gcagatgtat ttactacctt
     6601 gaaacgaatc atcctggtga cattcatcat tgagaccatt ggaggggttc tcatttatac
     6661 aagcctggat acgaatacaa ttccaccagt tggggaacgc atattttttt ccatctttca
     6721 tgccgtttcg gggttttgca acgcaggttt ttccacactg cctaatagtt tgtacgagcc
     6781 tggttttcgc tttaattatc cactgcacct ggtcattgct acattattta ttattggtgg
     6841 aattggattt ccgatccttt ttaatttttc aaaatactta aaatataaag ttgtcaagca
     6901 aatcctgcgt attcgtgagc agaaaacgga tatttattta agccgaataa ttaatatcaa
     6961 cagcaggatt gtaattatta ccacgatcgt tctaattaca ttaggaacta ttctttttta
     7021 tttttttgaa tatgataaca ccctggcaga acaccatggt tttggtaagg tagtaacagc
     7081 tttctttggc gcagtaacgc ccaggacagc cggttttaat tcagttgaca catcagtatt
     7141 aaatttttgg accataatgt ttgtttttct gttgatgtgg attggtgcat cacccgcttc
     7201 caccggaggg ggtatcaaaa caagtacgtt ttccatcggc atcttaaatt ttattagcct
     7261 ggccagaggc aaggatcgca tagaatattt tggcagggaa atagcagaag tatcagtaag
     7321 aagggctttc gcaatcattt ctttgtctct aattgtaata ggtgcggctg ttttcttaat
     7381 atcgtctttt gatagtgata aggacttaat cctaattgct tttgaatgtt tctctgcata
     7441 cagtaccgtt ggtttaagcc ttggcataac cagcgagctg agcagtgcaa gcaagctggt
     7501 aatcataatc accatgttca tcggcagggt aagtatgctt actatactga tagcgttatt
     7561 gagaaaagta cggtacatga aatacaggta tccgaccgaa gaaattctta taaactaaaa
     7621 attaaacatc catgaagtgc attattattg gtcttggcaa ctttggttca tcattatcag
     7681 aaaaacttac acagatggga catgaggtga ttggcgtaga cagcaaaatg agccgggtgg
     7741 aagccctgaa ggaaaagatc acccacaccg tctgccttga ttgcacggat tcgcaggctg
     7801 ttagccattt gccactaaaa gatactgata ttgtaattgt ctgtattggt gaggatgaag
     7861 gtgataatat catggcaaca gcattaatga aacggctgaa ggtcaaacgg ctgatcagca
     7921 gggcagtttc acccttgcat gaaactgtac tggaagcaat gggagttgat gagatcgtgc
     7981 atccggaaga agaaacagca gataggtggg caaaaaaatt aaatattagc ggggttattg
     8041 actcttttga tcttaccggg gaatatagca taatagaagc cacagtgccc ccgcggtacg
     8101 tgggcaaaac acttcttgat gcaggcttcc ggaaaaatta tgatgtggtg gtgttaacaa
     8161 ctataaaagt aactgaagaa gataatctga ttggcattac ccgcaagatt taaaaagttc
     8221 agggagtggc ctcttcaaga acactattaa gtgagggaga tattctggta atgtacggta
     8281 atattaaaga cattgaaagg ttgttaaagg aagagtgaaa gaactaaatt tccgtctcat
     8341 aacctgagta atgtatcgta taataagacc tgttttgttt atcattttga gcgcaataat
     8401 ttatttttcg ctgctgatct tgttttatca ttttgaattt gaagttcccg gatcgggggt
     8461 aagaaatttt gttgatgtga tatggtggtc agtggcaaca atagcgaccg ttggctacgg
     8521 cgatattgtt cctgtgacga cagagggccg tacaattgga tatgtttttc tgtttttaag
     8581 tgtaaccgtc tacgcctatg tgatcggtaa agtatccaca agtataaagt cacattataa
     8641 taacaaaaag ctggggttcc ttggaacaaa cttcacgaac catactgtga ttatcggatg
     8701 ggatgacttt gggaaaaaag tagttgatca attgataaaa gccaaccaga aagtagctat
     8761 tgttacagaa gagaggaatt atgttgacca gatctatcat ttgtacagtt caaaagctgt
     8821 attcgtattg tttgccagct gcaccaatta taaatacttt aacaagctaa atattgccga
     8881 atgcaatatt gtatttctaa acaatggaac cgacaccgat aacctggtgc atttactaaa
     8941 tctgaagaga catttccccg gattggaata cattgtgaat gttgataacc ccgaccttaa
     9001 aaataatttc tacagggagg gagtgaagta tgtgatatca ggtgatgaac tggcttccaa
     9061 attgatggcc agctatatct ttgaaccaga cgttgctttt ttcaatgaag aaataatcac
     9121 ctttgcagat tctgatgata agtatgatgt gaaacaattc aaggtgttgg atagaaatcc
     9181 ttttctaaac cgtgagtaca gcaaagtttt tttcgacctt aaaagagaat atagagctat
     9241 attagttggg atttcccggg aaatgaacgg aaagtatatg cttaagaaga accccgctga
     9301 cggaacgatt atacttgccg gtgattattt gcttgtgata gctaatcgtg ttgtggctga
     9361 aaaattatcg aagctattcg gagtaaagga aggcgtgaat taacaattca attttttgac
     9421 acaccacata ataacagctg catttctatt ttcagaacca cccctaaccc accttcggtc
     9481 ggcaggccca accttaaaaa aattgtcccc cttgttttaa gggggactac agggggtttt
     9541 gactaccatt ttaagaacca ctgtctcgtc ctgaaatagg ttgacaataa agtcaaccaa
     9601 aatgaatcac atagaattaa aagaaagtat cattgccgaa tatttatcca caggattaag
     9661 tcttcgtaaa ttggggatca agtacggatt aaatttccgt aatatccatc gctgggtaat
     9721 gcagtaccaa ggtcgcatga agaaaccaac aacaagcaaa cgtgtaaagc ccaacccgga
     9781 cctggctcag gaaggcatgc ccactgatgt gaagaaactt caaacggaat tgcgcaaagc
     9841 ccggctcctc aatgaagttc tcaccgaagt aatcaatatt gccgaagagg aactcggatt
     9901 gcctatccga aaaaagtatg gcaccaagta gttttaagaa tagtgcaaag agcgccttcc
     9961 cgatctgttg ggatgctttg ttcattactt ggttatagtc gtcaggcgta ttatcagcat
    10021 attaaagttc aagagcaaga agctttgaag gaggatttac tcctacagga agcattgcgt
    10081 atccgcaaaa cacaaaaacg gattggcact cgaaagttat taatcatgat gattgatttt
    10141 atgcaacaac atactatcag cataggccgg gatgccttct ttgagatgtt gcgtaatcaa
    10201 ggattgttgg tcagaagaag gaaacgctca aagccccaga ccacgttctc taatcactgg
    10261 ctgcgcaagt atcaaaatct gattattggt tttgttccta atgcacccaa tcaactgtgg
    10321 gtaagtgata taacctatat ccatttagaa gaaggctttg cgtatctaag cttgatcacc
    10381 gatgcctaca gccacaagat tgtagggttt tatttgggcg aagacctctc tgctaagggc
    10441 tgcataatgg ccttgaggat ggcgctgaag aataatccat tacgtgataa tcttatccat
    10501 cattccgaca ggggcctgca atattgttgt catgattatg tggagctttt ggagggtaac
    10561 agcattaaaa tttcgatgac acagaatggt gatccacttg aaaatgcaat ggccgaaaga
    10621 gtaaatg
//
LOCUS       DASWHX010000024        18696 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_13470, whole genome shotgun sequence.
ACCESSION   DASWHX010000024 DASWHX010000000
VERSION     DASWHX010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 18696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18696
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13470"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(117..1061)
                     /locus_tag="VGA21_01275"
     CDS             complement(117..1061)
                     /locus_tag="VGA21_01275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649164.1"
                     /translation="MRMEEMRMEMKRIMKRYVFMVLALPVLLMGCDNGKLKDLEIRNN
                     ELTNQLEEMDSSMNSYLETISEIEDNLAVIKLKQNMIATNASKDVEFDHEMRKSLVND
                     LQAINSLMTTNKEKITELQNLLKNSNFQVAEFKKLVARVNTKLVEKDEEITSLTTRLE
                     DLAFRNQTLDDNVRFLTIRVDTLSAVNQVQIATIQNQKKLISRNLEELSSGFVATGTL
                     KELEEKQIIKKEGGILGLGAVSALNSEVDRDNFLRIDIQKTQTIPLMVKKVRVITNHP
                     QDSYKIEESENGELLASLVITNPDRFWNTSKFLVVRVN"
     gene            complement(1249..1740)
                     /locus_tag="VGA21_01280"
     CDS             complement(1249..1740)
                     /locus_tag="VGA21_01280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649165.1"
                     /translation="MKPFRKLAFALLIILPGISIRAYSQDYSSAVGIRVGSTAGISVK
                     HFINKTMSFEGQLESIWRGINIGGLVEFYTPAFSIPNFKFYGGFGGHFGSWRGYGNHP
                     WFYEDRDYHSVVGVDGILGFEYTFDDIPLNIGIDWKPGFNIVEDAGPWVDNIGLSIRF
                     VIK"
     gene            complement(1878..3248)
                     /locus_tag="VGA21_01285"
     CDS             complement(1878..3248)
                     /locus_tag="VGA21_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009580796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX9649166.1"
                     /translation="MSELKIFVVEDDEWYSELLTYNLSLNPDYQVMKFKSGKECIDAL
                     KHNPDIITLDYRLPDMEGEKVLKEIKSFKQDIEVVIISEQESIETAVSLLRLGAYDYL
                     VKTKDIRDRLLNVVQNIRNQRGLQKKVETLQKEVEVKYDFKRSLIGNSEPMKKVFELI
                     DKAIKTNIVVMITGETGTGKELAAKAIHYNSTRKKGNFVPVNMAAVPRELVESELFGH
                     EKGAFTGAQARRIGKFEEADGGTLFLDEIGEMDLSVQAKLLRALQEKEITRVGSNKPV
                     KTDCRIIVASNRNLQEEVKQGNFRDDLYFRLFGLTINMPPLRERENDRLMLARFFLEN
                     FCKENDLLLKVMSRSAQKMLLDYSFPGNVRELRSIIELSAVMSNEVEILPEDITFGSS
                     DDSFSEDTNRELTLREHTHKIVKSYLNKYDNNVKLVAKKLDVGFSTIYRMLKEMEEHP
                     HEKQLN"
     gene            complement(3259..5838)
                     /locus_tag="VGA21_01290"
     CDS             complement(3259..5838)
                     /locus_tag="VGA21_01290"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9649167.1"
                     /translation="MKGKILAGFVLIFFAISLVGYYSFESLTRLVSTVEEKSRPNPKN
                     LQMKEILFDLSNAESSVRAYTITLDANYLIPYYETATTIERKLSDLYELCHDDEHEII
                     WLDSLSDLIDHKYFVMNDLIVMKQDDKRADILNQVLENIDRYDRALLDLELIDRSDNR
                     DDLKVLPDVPPFETSQVDDEIKNKRPNIFQRIFGTRNKERESSEIAAVGSASDSTSLP
                     QITLAERNLSTAKPKGSKELDAFKEKLVSAVAQMRQNEKSSVESMMAREIELFELDNE
                     IMTRIRSLMNKFEERERQISLEQSQSAEIMTQMTKNRIYWIGGGSLALFTILVIIIIR
                     DVSVNNQTRRKLQDAKDEAEKLAKVKEEFLSNISHEIRTPINSILGFARQLEKTSMDT
                     RQKAYLNVLSKSSEHLLALINDVLDYSKLESGNVSLEEIGFKPLVCIEEVKEALQPFA
                     ESKFLKLEIGVDNVPEILIGDPVRYRQILINLVNNAIKFTKSGTITIKVSVNIGANRC
                     KLKTSIIDTGIGIPPSKIFAIFKTFNQVDNSTAREYGGSGLGLSICKKLVDAQNGKIW
                     ATSNVGQGSAFNFEIIYPIGTEKDLPETGKQLTPAKGIIKEKIILVVDDEKYNLLLFR
                     TILENNNAKVITTDRPHDVQQILKEKPVDLILMDIQMPGLDGITLTKKIRAESKSTIP
                     ILALTATTNNTERAKCVEAGFDGFLVKPIEESILINSIANFIGWDQLEIQTKGVADTN
                     ELDLNNLFEIGNNDLDFVYNMLVLFINNFSNGMKELKEKLNEGNKQDLASVAHKMVPP
                     CRHLGLNDIVKILKSIEGAGRNEMEITELNLHIKNLEKDGKKIIQQLESEVERIKPHK
                     IKS"
     gene            complement(5972..6715)
                     /locus_tag="VGA21_01295"
     CDS             complement(5972..6715)
                     /locus_tag="VGA21_01295"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX9649168.1"
                     /translation="MESFIHILGCGDAFASGGRANTSFLVSHLGKKILLDCGSTTLTK
                     LKACGYCFQEISGIAISHFHGDHFGGIPNLILDAEFGNHKSKGFFITSPKEGKKRISD
                     LMECLYPGTSNLIDKQNITFHYYIDKEEIDLDFCKISALPVVHTPESYPHGIRLTIDD
                     FIIAFSGDTMWTDNLIELSESADIFIVDCNFLTNRGVNHMDYETLTQNLHRLNAGKIY
                     LTHMGEEVLNSAEVEIEKLNDGQFILLSK"
     gene            complement(6830..7075)
                     /locus_tag="VGA21_01300"
     CDS             complement(6830..7075)
                     /locus_tag="VGA21_01300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649169.1"
                     /translation="MGSGIKRYTKPVFFPVYGILIGYVIMWIVKWTTGIIPYQYIVFK
                     AGDHGFTMADIFLITGALTGGIIWYIKTKGKEPTDEP"
     gene            complement(7083..7403)
                     /locus_tag="VGA21_01305"
     CDS             complement(7083..7403)
                     /locus_tag="VGA21_01305"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX9649170.1"
                     /translation="MDTYKLDEIEAKEVFQGYLGKFIHSQNNTFAYWKIKANSPLPEH
                     SHHHEQVVNVLDGIYVLKVNGAEHVLEAGSVLVIPSNVKHSGYSQTDCTILDVFNPVR
                     EDYL"
     gene            complement(7409..8194)
                     /locus_tag="VGA21_01310"
     CDS             complement(7409..8194)
                     /locus_tag="VGA21_01310"
                     /inference="COORDINATES: protein motif:HMM:NF013328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HEX9649171.1"
                     /translation="MPELPEVENYRKYLEGTSLGKTIGELWIDDARLTKPGKKIFSNR
                     LKKNRFTSVSRIGKYLFVNLANGGCMVMHFGLTGNLSFFNNREDQPKYTRVLFQFTDG
                     SYLAYICKRKFGWLSYEKNLEQYLANSRLGIDATQLTFKDFFEKISGRNAPVKSLLLD
                     QSIAAGVGNWIADEILYQSKIHPETKTKSLERKEIRTLFNKLKKIIEISLKTEANYDK
                     FPKHFLIHNREPGGKCYYTGKPLIRIVVGGRGSFISPTIQIKK"
     gene            8271..9314
                     /gene="mnmH"
                     /locus_tag="VGA21_01315"
     CDS             8271..9314
                     /gene="mnmH"
                     /locus_tag="VGA21_01315"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF008750.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HEX9649172.1"
                     /translation="MKIRIEQFLEMASASPVLDVRTPAEFAIGHIPGATNFPLFQDEE
                     RKIIGTIYKNHGHIKAVLAGLNFVKDRMTIMINEAMNYSHNEAILLHCWRGGMRSESV
                     AWLLNSARIPTHTLSGGYKSFRNYALAFFEQKLNLAVISGPTGAGKTEILQKLKDKGE
                     QVIDLEKLAEHKGSVFGGIGKEQQPSSEHFQNLLFQEAKYFDLAKRVWIEDESLLIGR
                     VSVPEGLWKQMVRAPRVRILINKNTRIQRLVNDYGSQDMSKLADGITRLEKRLGNKSM
                     NDALHALKNGNLDETADILLTYYDKAYLNSFEKKKDTEWIRIESDDFSINSIAEQLIT
                     KAYPQTSEANYDR"
     gene            9304..10341
                     /gene="selD"
                     /locus_tag="VGA21_01320"
     CDS             9304..10341
                     /gene="selD"
                     /locus_tag="VGA21_01320"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015333585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HEX9649173.1"
                     /translation="MIDEKIRLTQFSHGAGCGCKISPEILEDILKKDAEVTPFPGLLV
                     GNDTKDDAAVFDLGNGISAISTTDFFMPIVDDPFDFGQIASVNAISDIYAMGGKPIMA
                     IAILGWPVDKLSPQIAKQVIRGAQKICSEAGIPLAGGHSIDNPEPLFGLAVSGIVKNK
                     NLKRNNTAKPGDILYLTKPLGIGIITTAQKKGIVKQKDLEAAVELMLTMNSLGTKLGE
                     LPYVNALTDVTGFGLLGHLLEICEGSNCSAKIEFSKVPLLKNLHYYLEQKSFPGGTTR
                     NLDSYGEKIQLTDEHVKLILADPQTSGGLLISVDQSAKSEFEDFLERNSIKPKAIGQM
                     VLRENKGITVV"
     gene            complement(10345..11205)
                     /locus_tag="VGA21_01325"
     CDS             complement(10345..11205)
                     /locus_tag="VGA21_01325"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX9649174.1"
                     /translation="MKKRLLPVIAIGLVITMWASAFVGIRSALESYSPFHLALFRYLI
                     ASAIMVVAGYLMNIRLPESKDIPAILFIGFIGITVYHVALNYGEITVNASAASFIVNS
                     VPLITAVFAILFLKERISKYGWLGLLGGFTGVSIIVLGESNGLSLSGGALVLVVAAIS
                     LAAFFVLQKPFLKKYKPLEFSTYVIVAGTLFMLFLSPGLLQSIENARLEHTVSVIYLG
                     IFPGAAGYILFAWVMNRYTATKSTSFIFLIPIMTLFLSWIWLGEIPAMLSLVGGGLAL
                     GGVYFYQKVG"
     gene            complement(11274..11657)
                     /locus_tag="VGA21_01330"
     CDS             complement(11274..11657)
                     /locus_tag="VGA21_01330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649175.1"
                     /translation="MIILANKKFRLTANSNVRDIGTNTIFTLNQEGDLLVGKYEGGKV
                     LHGNFLAKLVGHSAFEKHFHHYNNKGALVSGVSKGTIEVLPDGRLRLREFWNEDSKDG
                     DAIYDEVNASHSIPQEYYFNLSLNK"
     gene            11756..12676
                     /locus_tag="VGA21_01335"
     CDS             11756..12676
                     /locus_tag="VGA21_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009582819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX9649176.1"
                     /translation="MNLEIRHLRLIEAVAEEGSLTKAVDRLFLTQSALSHQLKEIETQ
                     LGSPVFHRVNKKLVLTGVGKIVLKLAKRILKEMEDTEIQIRSLVNGDTGAIRLATECY
                     TCYHWLPPLLKQFHREFPNIEVSIHPEFTSNPIRELLKGKIDLAITSLPLEDSNVVYR
                     ELFRDEMVALVSADHSWAGKEYVNPKDFTDESLIIYEKPLEDSVFYRQFLEPDGIRPK
                     KLIPIQLTEASIELVKAKMGIKVMARWAITPYFDPAKLATVKIGKKGLNRTWYVAMLK
                     KSGSASYLDFFIEHLCLTCLMKEQYPAVYA"
     gene            complement(12633..13208)
                     /locus_tag="VGA21_01340"
     CDS             complement(12633..13208)
                     /locus_tag="VGA21_01340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649177.1"
                     /translation="MRAVRDKVDISKAQNLLKDGLFFKRKIPDKVELLYLGFHLFKVT
                     VDPGNSAVKSEHVLIDVLTGAFSFLNSENILPFSDETQNYHSIKIDKDDAKEIAKREY
                     AFHLLHRNLRTKLKAEVRSVEFEETILYPFWVGYFIHFHHYSLEVIDAINGQKQSGAI
                     KKALIQYLADEFNSKTESMRKQPDIALSLNR"
     gene            complement(13208..13402)
                     /locus_tag="VGA21_01345"
     CDS             complement(13208..13402)
                     /locus_tag="VGA21_01345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649178.1"
                     /translation="MSEEPKNDKKFMRVISLFLLFFSGLVFISAAISSTPSAVITNVI
                     CGLLLAIISAIFYYGYKRVA"
     gene            complement(13606..15150)
                     /locus_tag="VGA21_01350"
     CDS             complement(13606..15150)
                     /locus_tag="VGA21_01350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Members of the Solute:Sodium Symporter (SSS), TC
                     2.A.21 as described in tcdb.org, catalyze solute:Na+
                     symport. Known solutes for members of the family include
                     sugars, amino acids, nucleosides, inositols, vitamins,
                     urea or anions, depending on the system; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/solute symporter"
                     /protein_id="HEX9649179.1"
                     /translation="MNLAIIDYIIIIFYLLGILFLGVYFRRYVHTSQGYFLAGRMLPF
                     WAIAFSIVGSDIGVTDFIGLSGEAYKHGIVAANYDWIGSVPAMVLAGLVFIPYYWRNG
                     VYTIPEYLGLRYNIYVRSFSAIIWLIVISLNIGIMFWIAGGIISGLLGWPLALSIMLM
                     AIIVGVYTIFGGLSAVVMTDVVQVIIMMIGSSAILILGFIELGGWNNMVGIIQSRGAE
                     YEHHFNLVLPMDTNSPYPWTGILFGLTLVLAPAYYIGNQNIVQRCLGASDEWNAKAGM
                     LGGAFLKTLVPLFIVFPGIIALALYPGLPDSDRTLLHLVEHLLPPGITGLVLAAFIAA
                     LMSSIDSFLASTSTIFTKDLYQTFIRKHAIDSHYLKVGRWTTAVILIIGMISAPLTDY
                     FSGVYTYFQTLNSFIQGPLLAILLLGIFWKRTSRPAGIFGLLGGVSLALLMFIYSEYL
                     FSLNDPYLYIAWWSFCGAFVITILVTLVTKPPDVRSMRGLIFGLSQNDDELQKIISDK
                     LGKSDV"
     gene            complement(15194..15958)
                     /locus_tag="VGA21_01355"
     CDS             complement(15194..15958)
                     /locus_tag="VGA21_01355"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020527424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HEX9649180.1"
                     /translation="MFLAADIGNTNIVLGFKQGDTWSHILRIETSDPTEKYALIISDF
                     VLERNIDSSLIESVALSSVVPWLTDPVIKILENLTQQRVYSLGPEFYHTLPVKIRNPE
                     EMGTDLIANSLAAYNSARSKCLVVDFGTALTFTSVGEDGEILGVAIAPGLKTAMKSLS
                     KSTAKLPEIPLVIPESALGKNTIHAMQAGILLGYTGLVEKMIAKIKSEIGEPVRVYAT
                     GGLSSILSNLNYLFDEVNANLTLEGLHEFHKLVSRK"
     gene            complement(16006..17685)
                     /locus_tag="VGA21_01360"
     CDS             complement(16006..17685)
                     /locus_tag="VGA21_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012926919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HEX9649181.1"
                     /translation="MDSREKTLAGNFIIQRIEKDLKEGKNDGRVHTRFPPEPNGYLHI
                     GHAKSICLNFGIAREYNGKCNLRFDDTNPETEEVSYVNSIKKDIKWLGFDWDNEFYAS
                     DYFDTFYEYAVRLIEKGKAYVDDQSQEIMSKNRGVPTKPGIESPFRNRSIEENLNLFE
                     EMKNGLHPEGSRALRARIDMSSPNMHLRDPVLYRIKKTPHHRTGDKWCIYPTYDFAHG
                     QSDSIEKITHSLCTLEFEVHRPLYDWLVREIEIFTPQQIEFARLNLTYTIMSKRKLLQ
                     LVNEKHVKGWDDPRMPTISGMRRRGYTPASIRNFSDRIGVAKRDGVIDLSLLEFSVRE
                     ELNKESNRVMAVLYPIRLIITNYPVGKTEMLPAKNNPENPDSGTRELPFSKTLLIERE
                     DFMENPPSKFFRLGPDRMVRLKYAYIIKCTGYEKDTEGKITEVRCEYFPNSRSGSDTS
                     GLKTKGVIHWVSEQDAINAEVRLYDRLFVVEEPGSVKDKSLKDMLNPASEIILKNAKV
                     EPSLKEAKELDKFQFERTGYFCVDGDSLPGNLVFNRTVTLKDSWIKEKAKI"
     gene            17844..18161
                     /locus_tag="VGA21_01365"
     CDS             17844..18161
                     /locus_tag="VGA21_01365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649182.1"
                     /translation="MNEVETNAISGIIMQTFLRKKLLIYKEVMQTLDQYRDVWQDNED
                     FCKAVDRFSVVSQRESLDLYLADNILKYTIDFRMFGFRKNNLGFFQQYFRARVLAKRE
                     LVK"
BASE COUNT         5637 a         3874 c         3397 g         5788 t
ORIGIN      
        1 ttatttcagt attcagttgc ctgcaagata aaaaaaatcc tccaccggga ggattttttt
       61 aagaaagtga aggaccggcc ggggctaaac ccaatccccg gccggttatt ggtaggctag
      121 ttgaccctca ctacaagaaa tttagacgta ttccaaaagc ggtcaggatt tgtaataacc
      181 aggctcgcaa gtaattctcc attttcagat tcttcaatct tataggaatc ttgcggatga
      241 ttggtaatta ccctaacctt cttcaccatt aatggaatag tctgagtctt ttgaatatct
      301 atacgaagga aattatctct atctacttca ctgtttaaag ctgacaccgc gcctaatcca
      361 agtattcctc cctccttctt aataatttgc ttctcttcaa gttcctttag ggtgccggtt
      421 gcgacaaaac cggaagacag ttcctcaagg tttcgggaaa tcaatttctt ttgattctgg
      481 atagtcgcaa tttgcacttg atttacggcc gataatgtat ctactctaat ggtcaggaat
      541 ctaacattgt catccaatgt ttgatttctg aatgccaaat cttcaagtct ggtagttaag
      601 gaagtaattt cttcatcctt ttctacaagt tttgtattta cccgtgctac cagtttctta
      661 aactcagcaa cctgaaaatt tgaattctta agtaaattct gcaattcagt gatcttttct
      721 ttattggttg tcattaaaga attgattgcc tgtaaatcat taacaagact ttttcgcatt
      781 tcatgatcaa actcaacatc cttgcttgca ttagttgcaa tcatattttg cttcagcttt
      841 attacagcca gattatcttc aatttcgcta attgtttcca ggtatgaatt catactggaa
      901 tccatttcct ccaactggtt ggtgagttca ttattcctga tttcaagatc ttttaatttc
      961 ccattatcgc atcccatcag caacaccgga agtgccaaca ccataaacac atacctcttc
     1021 attatccgtt tcatttccat tctcatttct tccattctca tttctctatg cttttatatt
     1081 tactcttaat tttaactttg gttgatactt tataatgtca aaatgtactc caaaatacta
     1141 tctgcctcat attcaaataa ttacaataat tccgaaacga tttttgttgt cattttggga
     1201 atttatttgg caaaaaggga tagaattacc cgctaacatc acctgtattt atttaataac
     1261 gaagcggatg gataatccaa tgttatcaac ccacggtccg gcatcttcaa caatattgaa
     1321 tcctggtttc cagtcaatgc caatgttcag tggtatgtca tcaaaggtat attcgaagcc
     1381 aagaattccg tcaacaccta cgaccgagtg ataatccctg tcctcataaa accaggggtg
     1441 gttgccataa cctctccatg atccaaaatg ccctccaaag ccaccgtaga atttaaagtt
     1501 tggaatacta aacgctggag tatagaattc taccaatcca ccgatattaa ttccacgcca
     1561 gatactttcc agttgtcctt caaacgacat tgttttatta ataaaatgtt taacagaaat
     1621 acctgcagtt gagccgactc tgattccaac agcagaggaa tagtcctggc tataagctct
     1681 gatagaaatt ccgggcaaaa taattaataa agcgaacgca agttttctaa aaggtttcat
     1741 aaactatttt ttggtgatac caagtagtac aagcactatt cctgtttaca atttactgta
     1801 tatcaggtgc ttaacgcata attcaaaatt tgcagattgt cgaaatggca ggataatttt
     1861 cgatttgaaa tcaggagtta atttagttgt ttttcatgtg ggtgttcttc catttccttg
     1921 agcatccgat aaattgtaga aaaaccaaca tcaagctttt ttgcaaccag ctttacgtta
     1981 ttatcatatt tattaagata agattttacg atcttatgtg tatgttccct caaggtaagt
     2041 tcacggtttg tatcctctga gaatgaatca tcagacgagc cgaaagtaat atcctccggc
     2101 agtatttcta cttcgttaga cattacagcg gataattcta taattgagcg cagttcgcgg
     2161 acatttcccg gaaaagaata gtcgagtaac atcttttgcg cactacggct cattacttta
     2221 agcagcagat cattttcttt gcaaaagttt tccaggaaaa acctggcaag catgagcctg
     2281 tcattttctc tttcccgtaa aggcggcata ttgatggtta atccaaaaag acggaaatag
     2341 agatcatccc tgaaattgcc ttgcttcact tcttcctgaa ggttacggtt cgatgccaca
     2401 ataattctgc aatccgtctt tacaggtttg ttagaaccaa cccgggtaat ttctttttcc
     2461 tgaagtgccc gaagcaattt agcctgaact gaaagatcca tttcacctat ttcatctaaa
     2521 aacaatgtgc caccatctgc ttcctcaaac ttcccgattc tcctggcctg agcaccggta
     2581 aatgctcctt tttcatgacc aaataattca gattcgacta actctcgcgg aaccgctgcc
     2641 atatttaccg gtacaaagtt gccttttttc cttgtggagt tatagtgaat tgctttggca
     2701 gcaagttctt tacctgtgcc cgtttcaccg gtaatcataa ctactatatt ggttttaatt
     2761 gctttatcta tcagctcaaa aactttcttc attggttcac tatttccaat cagtgagcgt
     2821 ttgaaatcat atttaacttc aacttctttt tggagggttt caactttttt ctgaagaccg
     2881 cgttgatttc gaatattctg cacgacatta agcaggcgat cccggatatc tttagtcttc
     2941 accaggtaat cataggcacc taacctcaat aatgaaacgg cggtttcaat actttcctgc
     3001 tcactaataa taactacctc aatgtcctgt ttaaaactct tgatctcttt caaaaccttc
     3061 tcaccttcca tatccggtaa gcggtagtca agggtaataa tatccgggtt atgcttaaga
     3121 gcatcgatac attcttttcc tgacttgaat ttcataacct ggtagtcagg gtttaaggaa
     3181 aggttataag tcagcaattc actgtaccat tcatcatctt cgactacaaa tatttttaat
     3241 tcactcattt ttaagaatct aacttttaat tttgtgaggt tttatacgtt caacttcgct
     3301 ttctaattgt tgaattattt tcttgccgtc tttttctaaa tttttaatgt gaaggttaag
     3361 ttctgtaatc tccatttcat ttctgccggc accttcaatt gatttcagaa tttttacaat
     3421 gtcatttaaa ccgagatgcc tgcatggcgg aaccatctta tgcgcgactg atgccagatc
     3481 ttgcttattt ccctcattca gtttttcttt caactctttc attccatttg agaaattatt
     3541 gataaaaaga acgagcatgt tatacacaaa atcaagatca ttattgccaa tttcaaataa
     3601 attgtttagg tcaagctcat ttgtatccgc gacacctttc gtctgaatct caagctggtc
     3661 ccaaccaatg aagttagcaa ttgagttgat cagtatcgat tcttcaatag gtttaaccag
     3721 gaatccatca aatcctgctt caacacattt tgccctttct gtattgttgg tggtagcggt
     3781 aagtgcaagt atcggaattg tgcttttact ttcggcccgt attttttttg ttaatgttat
     3841 accgtcaaga ccgggcattt ggatatccat gagtatcagg tcaacaggtt tttctttcag
     3901 aatttgttga acatcatgcg gtcgatccgt ggtgattact ttagcattat tgttctcaag
     3961 aatggttctg aataacaaga gattgtactt ctcatcatcg accaccaaga ttattttctc
     4021 tttaattatt cctttagcag gggttaactg cttaccggtt tcaggcaaat ctttttcggt
     4081 gccgattggg taaatgattt caaaattgaa agcactcccc tgacctacgt tgctggtcgc
     4141 ccaaattttt ccgttttgag catcaaccag tttcttgcaa atgcttaagc ctaatcctga
     4201 tcctccatat tcacgagcgg tgctgttgtc tacctggttg aaggttttga aaatggcaaa
     4261 aattttactc ggcggaatac ctattcctgt atcaataatg ctggttttaa gtttgcaccg
     4321 gtttgctcca atgtttacac ttactttaat tgtaattgtg ccggacttgg taaacttgat
     4381 tgcattattt accaggttga tcaaaatctg cctgtacctt acaggatctc caataagaat
     4441 ttcaggaaca ttatccacac caatttccag ctttaaaaat tttgattccg caaagggttg
     4501 taatgcctcc ttaacttctt caatacaaac cagtggctta aagccaattt cttccagact
     4561 aacattccca ctttcgagtt tagaataatc cagaacatca ttgatcagag caagcaaatg
     4621 ctcagaggat ttggaaagta cattgaggta agccttttgc ctggtatcca tggatgtttt
     4681 ttccaattgc ctggcaaaac ccaggataga gttaatcgga gttcgaatct cgtgactgat
     4741 atttgagagg aattcttctt ttactttcgc taatttttcc gcctcatcct ttgcatcctg
     4801 cagttttctt cgggtttggt tatttaccga aacatctctt ataatgatta tcaccaaaat
     4861 tgtaaacaac gccaatgaac caccccctat ccaataaatt ctgttcttgg tcatttgggt
     4921 cattatttca gcgctttgtg attgttctaa tgatatttgt ctttctctct cctcaaactt
     4981 gttcataaga ctccgtatcc gggtcattat ttcattatcc agctcaaata attctatttc
     5041 cctggccatc atgctttcaa ctgaactttt ctcattttgt ctcatttgcg ctaccgcgga
     5101 aactaacttt tccttaaatg catctaactc ttttgaaccc tttggtttag cagtggaaag
     5161 gtttctttct gccagagtaa tttgaggtag tgaggttgaa tccgaggcag atcctaccgc
     5221 ggcaatttcc gaactttccc tttccttatt tctggttcca aatattcgct gaaaaatatt
     5281 tggtctctta ttctttattt catcatcaac ctggctggtt tcaaaaggtg gaacatccgg
     5341 caataccttt agatcatctc gattatctga gcggtcaatg agctcaagat ccagtaatgc
     5401 cctatcatac ctgtcgatat tttcaagaac ctgattaagt atatctgctc tcttgtcatc
     5461 ctgcttcatc actataagat cattcataac aaagtactta tgatctataa ggtcagataa
     5521 ggaatccagc caaattattt catgttcgtc atcatggcag agctcatata agtctgacag
     5581 ctttctttca atggttgttg cggtttcgta ataaggaatc aggtaatttg catcaagggt
     5641 tatggtataa gcccttacac tcgactccgc gttggataaa tcaaaaagta tctccttcat
     5701 ttgaagattc ttcggatttg gtctcgattt ctcctcaaca gtggaaacta accgggtaag
     5761 actctcaaaa gaatagtaac ccaccagaga gatggcaaaa aatattaaaa cgaatccggc
     5821 aagaatttta cctttcaaag gttttaagtt aattcagata atcttcaagg ctcaattaat
     5881 gttccagcaa aacacagttc aatttaagca aaatttagcc cttttctatt aaaaatttgt
     5941 caaaatgaga catttggaat gggtgatatt gttattttga caacaagatg aattgtccat
     6001 cattaagttt ttcaatttct acttccgccg aattcaagac ttcttcgccc atatgtgtca
     6061 agtatatctt tcctgcattt aatctatgca gattttgcgt cagggtttca taatccatat
     6121 gattgacccc acgatttgtt aaaaaattgc aatccacaat aaaaatatcg gcactttccg
     6181 aaagttcaat gaggttatca gtccacatag tatctcccga aaaagcaatg ataaaatcat
     6241 ctatagtcaa cctgattccg tgtgggtaag attccggagt atgaaccacc ggcagagcac
     6301 tgattttaca aaaatccaaa tcaatttctt ctttgtctat ataatagtga aaagtgatat
     6361 tttgtttatc tatcagatta cttgttccag gatacagaca ttccattaaa tcagagattc
     6421 ttttctttcc ttccttaggt gaggtaatga agaatccttt actcttatga ttgccaaatt
     6481 cggcatcaag gatcaagttg ggaattcctc cgaaatgatc accatgaaaa tggctgattg
     6541 caattcctga aatttcctgg aaacaatatc cacatgcttt aagtttggta agcgtggtgg
     6601 atccgcaatc cagaagtatt tttttcccca gatgactgac caaaaaactg gtatttgccc
     6661 tgcctccgga ggcaaatgca tcaccacatc caagtatatg aataaatgat tccatactaa
     6721 gcttacttaa taatgttcca aaatccgaat gtcaggagat tccattttct gctgtgtaaa
     6781 acattgccaa attcacgaag ccggaaaaga ttgatgtaag gactactctt tacggttcgt
     6841 ctgtaggttc tttacccttc gttttaatat accagattat cccgcctgtt aatgctccgg
     6901 taataagaaa aatatctgcc atcgtaaatc catgatcacc tgctttaaaa acgatgtatt
     6961 gataaggtat gatccctgtg gtccacttaa caatccacat tataacatag ccaatcagga
     7021 ttccataaac agggaaaaaa accggcttcg tatatctttt tattccacta cccatttttg
     7081 aattataaat aatcttctct taccggattg aacacgtcaa ggatagtgca atctgtttgc
     7141 gaatatccgg agtgcttcac attagacgga attactaaaa cacttcctgc ttctaaaaca
     7201 tgttctgcac cattgacttt cagaacgtaa atgccatcaa ggacattaac cacttgttca
     7261 tggtggtgtg aatgttccgg aagcggggaa ttcgccttga ttttccagta agcaaatgta
     7321 ttgttttggg aatgaataaa tttccctaaa taaccttgaa aaacttcttt tgcttcaatc
     7381 tcatcaagtt tgtaagtatc cataataatt acttttttat ttgaattgtt ggtgaaataa
     7441 aacttcctct cccacccact acaattctta ttaaaggctt gccggtataa tagcatttac
     7501 ctcctggttc cctgttgtgg atgaggaagt gctttggaaa tttatcatag ttggcttcgg
     7561 ttttcaaaga aatttcaata atctttttca atttattgaa aagtgtccgg atctcttttc
     7621 gctccaggga ttttgttttc gtttcggggt gaatttttga ttgatacaat atttcatcgg
     7681 caatccagtt tccaacaccg gcggcaattg attgatccaa cagtaaagat tttaccggag
     7741 catttctacc acttatcttt tcaaaaaaat ccttgaacgt taattgagtt gcatcaatgc
     7801 ccaggcggga attagccaaa tactgttcca gatttttttc ataggaaagc cacccgaatt
     7861 tccttttaca aatgtaagcg agatatgatc catcagtaaa ttgaaataat acccgcgtat
     7921 atttgggctg gtcttctctg ttgttgaaaa aacttaggtt cccggtcaga ccaaaatgca
     7981 taaccatgca gccaccgttt gcaagattca caaacaaata cttgccaatc cttgatacag
     8041 aggtaaacct gtttttcttc agcctattag aaaatatctt tttacctggc ttggtgagtc
     8101 gcgcatcatc aatccataat tctccaatgg ttttgcccaa tgaagtacct tcaaggtact
     8161 ttcgataatt ctcaacttca ggtagttccg gcattcaaac taaatattcg gtaataacct
     8221 ttaactttga ttgttctaag gtaagataaa gatccatatt agtttatcaa gtgaaaatac
     8281 gcattgagca atttcttgaa atggcctctg ccagccctgt attagatgtc agaacaccgg
     8341 cagaattcgc tattgggcat ataccaggtg caacaaattt tcctttgttt caggatgaag
     8401 aaagaaaaat tatcggaacc atctataaaa accacggcca cataaaagca gttctggcgg
     8461 gtttgaattt tgttaaagac aggatgacaa tcatgataaa tgaagccatg aattattccc
     8521 ataatgaagc aatactgctc cattgctggc ggggtggcat gcgctccgag agtgtggcct
     8581 ggctgttgaa ctcggcaaga ataccgactc atactctaag tggcggatat aaaagctttc
     8641 gtaattatgc cctggctttc tttgaacaaa aactcaacct tgcagttatc agcggaccta
     8701 ctggcgccgg gaaaacagaa attcttcaaa aattaaaaga taagggagag caggtaatcg
     8761 atttggaaaa actggctgag cataaaggat ctgtttttgg tggaattggt aaagaacagc
     8821 agccttccag cgagcatttt cagaacctgt tatttcaaga ggccaaatat tttgatttgg
     8881 caaaacgagt ttggatagaa gatgaaagtc tccttattgg gcgcgtgtcg gttcctgaag
     8941 gcctttggaa acagatggtc agggcaccac gggtaagaat actaatcaac aaaaacacgc
     9001 gtatacagag attagtaaat gactatggca gccaggatat gagtaaactt gccgatggaa
     9061 ttacccgttt ggaaaaaagg ctgggaaata aatcgatgaa tgatgctctc catgctttga
     9121 aaaatggaaa tcttgatgaa acggccgaca tcctgcttac ttattatgat aaggcttacc
     9181 tgaattcctt tgaaaagaaa aaagatacgg aatggatacg cattgaatca gatgacttca
     9241 gcataaattc cattgctgaa caattaataa caaaagcgta tccgcaaacc tctgaagcaa
     9301 attatgatag atgaaaaaat ccgcttgact cagttcagtc atggagctgg atgtggctgc
     9361 aaaatttcac ctgaaatact ggaagacatt cttaagaaag atgctgaagt aacgcctttt
     9421 cccggcttgc tggtcggaaa tgataccaaa gatgatgcgg cagtttttga tttaggcaat
     9481 ggtatttccg ctataagtac aacagatttc ttcatgccta tagtggatga cccattcgac
     9541 ttcggtcaaa ttgcttcggt gaatgcgatc agtgatattt atgcaatggg cggtaagcca
     9601 atcatggcca tagctatttt aggatggccg gttgataaac tttcacctca aattgctaaa
     9661 caggtaattc gaggggcgca gaaaatttgt tcggaagcag gcatcccttt agccggcggt
     9721 cacagtatcg ataatccgga accgcttttt ggtttggccg tttcgggcat tgtaaaaaat
     9781 aaaaatctga aaagaaataa caccgcaaaa cccggcgaca tactttattt gaccaaacca
     9841 ttgggtattg gaataattac tactgctcaa aaaaaaggaa ttgtaaaaca aaaggatttg
     9901 gaggcggcag ttgagttaat gctaactatg aactcgctcg gtacaaagct tggtgaactt
     9961 ccttatgtaa atgcgttgac agatgtcaca ggctttggct tgttaggtca cctgttggaa
    10021 atttgcgaag ggagtaattg ctcagctaaa attgaattta gcaaggttcc gttacttaaa
    10081 aatctacatt attatcttga gcaaaaatct tttcccggag gtactacgcg caatctggat
    10141 agttacggag aaaaaattca acttactgat gaacatgtca aactaatcct ggcagaccct
    10201 caaaccagcg gcgggttact catatcagta gaccaatccg caaagtctga atttgaagat
    10261 tttcttgaaa ggaattcaat aaaaccaaaa gccataggac aaatggtcct gagagaaaat
    10321 aaaggcataa ccgtggttta acccttaccc aactttctga taaaaataaa ctccacccaa
    10381 tgccagccca cctcctacca gggaaagcat ggccggaatt tcaccaagcc agatccaaga
    10441 taagaataaa gtcataatag gtatcaggaa aataaaactg gtggattttg ttgccgtata
    10501 cctgttcatt acccatgcaa aaagaatata tcctgctgct cccggaaata tacccagata
    10561 aataacagat actgtgtgct ctaatctcgc attttcaatt gattgcagga gtcccggtga
    10621 taggaataac ataaataatg tgccggctac aataacatag gttgagaatt ccagcggttt
    10681 atattttttc agaaatggct tctgcaggac aaaaaatgct gcaagtgaaa ttgccgcaac
    10741 caccagcacc agtgctccac cactcaatga aagtccgttg ctttctccca acacaattat
    10801 ggagactccc gtaaatccac ccagtaatcc caaccagcca tatttgctga ttctctcttt
    10861 tagaaataaa atggcaaaaa ctgcagttat aagcggaaca ctgttaacaa taaaactggc
    10921 tgcgctggca ttcacggtta tctcaccata attcaatgca acatggtaca ccgttatacc
    10981 tatgaatcca ataaataaaa ttgcaggtat gtccttgctt tcaggcaacc tgatattcat
    11041 caaatatccg gcaacaacca ttattgctga ggcaatcaga tatctgaata aagcaaggtg
    11101 gaatggagaa taggattcca atgcagatcg aatgcctaca aaggcggaag cccacatggt
    11161 aattaccaaa ccaattgcaa taaccggcaa taatcttttt ttcatattaa gtcatcaatt
    11221 aaaatcaatg gaaaccagca tgctttgaaa atatgagaac tggttattca gatttattta
    11281 ttcaaagaga gattaaaata atactcctgg ggaatagaat gtgacgcgtt cacttcatcg
    11341 taaattgcat ctccgtcctt agaatcttca ttccagaatt ccctgagtcg taatctacca
    11401 tccggaagaa cctcaatagt gcccttgctc actcccgaaa ccaaagcgcc tttattatta
    11461 tagtgatgga aatgtttttc gaaggcggag tgaccaacga gctttgccaa aaaatttcca
    11521 tgcaaaactt ttcctccttc atacttgccg accaataaat caccctcctg attcaaggtg
    11581 aagattgtat tagttccaat gtccctgaca ttcgaattcg cagtcagcct gaatttctta
    11641 ttggctaata ttatcatttt taaatctttt ttagcaaata aacgtagacc tgtgtacatt
    11701 tgatttatta atcagatgaa tagttttatt gattattatt aattctacta ataatatgaa
    11761 tctggaaatc agacatctta ggctaattga ggcagtggct gaagaaggca gtctaacaaa
    11821 agctgttgat agacttttcc ttacacagtc agccttaagc catcagttaa aggagattga
    11881 gactcaattg ggctcccctg tttttcacag ggtaaacaaa aagctggttt tgacaggagt
    11941 cggcaaaatt gttcttaagt tggcaaagcg aatcctgaaa gaaatggagg atactgaaat
    12001 tcaaatcagg agtctggtta acggggatac cggtgccatt agattggcca ctgaatgcta
    12061 cacctgctat cattggcttc cgcctctgct gaaacaattt cacagagaat ttcccaacat
    12121 tgaggttagc atccacccgg aattcacctc gaaccctatc agggagctgt tgaaaggcaa
    12181 gatagatctt gccattacct cgctgccgtt ggaagatagt aacgtagtct accgggagtt
    12241 attcagagat gagatggtgg cccttgtaag tgccgaccac tcctgggcag gtaaagaata
    12301 tgtaaatccg aaagatttta ccgacgagag tcttatcatt tatgaaaagc ctttagaaga
    12361 ttctgtattc tataggcagt ttctggaacc ggatggaatt cgtcctaaaa aattaatccc
    12421 aatccaatta acagaagcaa gcattgaatt ggttaaagca aagatgggta taaaggtaat
    12481 ggcaagatgg gctattactc cttatttcga ccctgcaaag ttggcgacgg taaaaattgg
    12541 caagaaggga cttaacagga cctggtacgt ggccatgcta aagaagtctg gcagtgcatc
    12601 ttatcttgat tttttcatcg aacatttgtg ccttacctgt ttaatgaaag agcaatatcc
    12661 ggctgtttac gcatagattc ggtttttgaa ttgaattcat ctgctaggta ttgaatcaat
    12721 gcctttttga tagccccgga ctgtttttga ccattaatag catctattac ttccaatgaa
    12781 taatgatgga agtgaataaa atagcccacc cagaaaggat aaagaatagt ttcttcaaat
    12841 tcaacgctcc tgacctccgc tttgagtttt gttctcaaat tccgatgcaa caaatgaaaa
    12901 gcatattctc tctttgcaat ttcctttgca tcatctttat caattttaat tgaatgataa
    12961 ttttgagttt catctgaaaa aggcagaata ttttcagagt taagaaaaga aaatgctccg
    13021 gtaagaacat caatcaaaac gtgttctgat tttactgctg aatttcccgg atcaaccgtt
    13081 accttgaaca aatgaaagcc cagataaagt agttctacct tgtctggaat ttttctctta
    13141 aagaataatc catccttaag taagttttgt gccttgctta tgtcaacttt atctcttacc
    13201 gctctcatta tgctaccctt ttataaccgt aataaaatat tgctgaaatg attgccagaa
    13261 gcaacccaca tattacattc gtgataaccg cggatggtgt actgctgatg gcagctgaaa
    13321 taaagaccaa gcccgagaaa aatagcagga acaatgaaat tacccgcata aatttcttgt
    13381 catttttcgg ttcttcgctc atattagaaa tagctataca acaaaatttg aacaatcact
    13441 aaaattaaaa tccagtacct gaaatctctc cctcgacgct gatttttggc atccttgaag
    13501 atatagcttt tcttcattag caaagcaaat atcagagatg aaagaaaagt aagaattaaa
    13561 aggattcttg ctccggatag agatacatca ttaagccaat caaaattaaa catcactttt
    13621 ccctaattta tctgaaatta ttttttgcaa ttcatcatca ttttgagaaa gcccgaaaat
    13681 taagcctctc atggaacgaa catcaggggg tttcgtcaca agtgtaacca atattgttat
    13741 tacaaatgct ccacaaaagc tccaccaggc tatatacagg tatggatcat ttagtgaaaa
    13801 gaggtactca ctataaataa acatcagcag agccaaactt acacctccca gtaatccaaa
    13861 aattccagca ggtcttgaag tgcgtttcca gaagataccc aatagcaata ttgccagaag
    13921 cggtccctga ataaatgaat ttaaggtctg aaaatacgta tacactccgc taaaataatc
    13981 ggttaatgga gccgatatca tcccaattat aagaatgaca gcggttgtcc accttcctac
    14041 tttcaggtaa tgtgaatcta tcgcatgctt cctgatgaat gtttggtaca agtctttggt
    14101 gaaaatagta gaagtacttg ccaggaaaga atcgatgctg gacattaatg cggcgataaa
    14161 agcagcaagt acgagaccgg taattcccgg aggaagtaaa tgttcaacaa ggtggagaag
    14221 ggtgcgatcg gaatcgggca agcccggata caaagcaaga gcaataatac cgggaaatac
    14281 tatgaaaagt ggtacgaggg tcttcaggaa ggctccacct aacataccgg cctttgcatt
    14341 ccattcgtct gaagctccaa gacatcgttg cacaatattt tgattaccta tgtaataagc
    14401 aggggctaat accagtgtaa gtccgaaaag aatccctgtc cagggatagg gtgaatttgt
    14461 atccatcgga agtaccaggt tgaagtgatg ctcatattct gcaccccggg actgaataat
    14521 gccaaccatg ttgttccacc ctcctaactc aatgaagcca agaatcaaaa tggcggaact
    14581 tccgatcatc ataatgatta cctggacaac atcggtcatt accaccgcgc ttaatccgcc
    14641 aaagattgtg tatactccga caataatggc cattagcata atggaaagtg ccagaggcca
    14701 accgagtaat ccggaaataa tgccccccgc tatccagaac ataatgccaa tgtttagtga
    14761 aataacgatg agccaaatta tcgcgctaaa gcttcgcaca taaatgttat atcgcaaacc
    14821 aagatattcc ggtatcgtat aaacaccatt tctccaataa tatggaatga ataccagacc
    14881 tgccagaacc attgccggta cagaccctat ccaatcgtaa ttcgcggcaa caataccatg
    14941 cttataggct tcacctgata acccaatgaa atcagttact cctatgtcag aaccaacaat
    15001 tgaaaatgca atagcccaga aaggaagcat tcggcccgcc agaaaatatc cctgggaagt
    15061 atgcacatat cttctgaagt aaactccaag aaaaagaatt cccagaagat aaaatatgat
    15121 aatgatatag tcgataatgg caaggttcac tgcttatttc aggagttatg attttaaggt
    15181 cagttttatt acattatttc ctggacacga gtttatggaa ttcatgaagt ccttcaagag
    15241 tcagattcgc gttaacttca tcgaacaaat aattgaggtt tgaaagaatg gaagataacc
    15301 cgccggtagc gtagaccctt accggctcac ctatttcaga tttaatttta gcgatcattt
    15361 tttcaaccag tcctgtgtat cctaataaga ttccagcctg cattgcatga atagtatttt
    15421 tccccaaagc actttccggg attaccaatg gaatttccgg cagtttggcc gtactttttg
    15481 aaagggattt cattgcagtt tttaatcccg gagctatcgc cacacccaaa atttctccat
    15541 cttcaccaac cgaagtaaaa gtcaatgctg ttccaaagtc caccacaaga catttgcttc
    15601 gggcagaatt ataggccgcg agcgaatttg caataagatc ggttcccatt tcctccggat
    15661 ttctaatttt taccggcaga gtatggtaaa actccggccc aagagaataa actcgttgct
    15721 gagtcaggtt ttccagaatt tttattacag gatcagttaa ccagggaaca acactgctca
    15781 acgccacgga ctcaattaaa gaggagtcaa tatttctttc aagaacaaaa tcagaaatta
    15841 tgagcgcata tttttccgtg ggatcacttg tttcaatgcg cagaatgtgg gaccaggtat
    15901 ccccttgttt aaatcctaaa actatattcg tatttccaat atcagcggca agaaacatgg
    15961 ctatactttt ctacttattt tctattcatt aaaatttatg aaaaactaaa ttttagcctt
    16021 ttcttttatc caggagtcct ttaaggttac cgttctgttg aaaacaagat tgcccggtaa
    16081 tgaatccccg tcaacacaaa aatacccggt tcgttcaaat tggaatttat ccaattcctt
    16141 agcttctttc aaggaaggct ctaccttagc gttcttaagg attatttccg aagcaggatt
    16201 taacatatct ttcagcgatt tatcttttac agagccgggt tcctctacaa caaataacct
    16261 gtcgtatagt ctgacttctg cattaatagc atcctgctct gatacccagt ggattacgcc
    16321 ttttgttttc aatccggaag tatcactgcc acttcgtgaa tttggaaaat actcacatct
    16381 gacctcagta attttacctt cggtatcttt ttcatatccg gtacacttaa ttatatatgc
    16441 gtatttcagc ctgaccattc tatccggacc taacctgaag aactttgaag gcgggttttc
    16501 catgaaatcc tccctttcga ttaagagagt ttttgaaaag ggtaattctc tggtaccact
    16561 gtccggattc tcaggattat ttttagccgg gagcatttca gtttttccaa caggataatt
    16621 ggtaataata agtctaatgg gatataaaac tgccattacc ctgtttgatt ctttattcaa
    16681 ttcttcccga acactaaatt ctaaaagaga taaatctatc accccatctc ttttagcaac
    16741 tcctatcctg tcgctgaaat tacgaatgga agcaggggta tatccacgcc tgcgcattcc
    16801 ggagattgtc ggcattcggg ggtcatccca gcccttaaca tgtttttcat ttaccagttg
    16861 aagaagtttc cgcttgctca taattgtgta agtgagattg agccgcgcaa attcaatttg
    16921 ttgaggagta aaaatttcaa tttctcttac caaccagtca taaagcggcc ggtgcacttc
    16981 aaattccaat gtgcagagcg aatgggtaat tttttctatg gaatctgatt gcccatgtgc
    17041 aaaatcgtaa gtaggataaa tgcaccattt atcaccagtt ctgtgatgcg gggttttttt
    17101 gatccggtaa aggacaggat cccgcaaatg catattcgga gaagacatat caatccgggc
    17161 acgtagagcc cgtgaccctt caggatgaag cccgttcttc atttcttcaa acaaattcag
    17221 gttttcttct atggaacgat ttctgaacgg actctcaatt ccgggtttag ttgggacacc
    17281 ccggtttttg ctcattatct cttgcgattg atcatcaacg taagccttcc ctttctcaat
    17341 caacctgacc gcatactcat aaaatgtgtc aaaataatcg ctggcgtaaa attcattatc
    17401 ccagtcaaat cccagccatt taatgtcttt cttaattgag ttgacataag agacttcttc
    17461 cgtttcaggg tttgtatcat cgaaacgaag gttacatttt ccgttgtact ctctggcgat
    17521 tccaaaattg agacaaatag actttgcatg accaatgtga agataaccat tgggttccgg
    17581 cgggaaacga gtatgaaccc taccatcatt cttgccctcc ttcaaatcct tttcaatcct
    17641 ttgaatgata aagttacctg ctaatgtctt ttccctggaa tccattaaaa gtggggtcaa
    17701 taccttaaaa aattcagatg caagtttagt gaaaaatggt caaagaaagg cttctgctaa
    17761 gattttcatt gagtgttaca atagtcctag ggtttgggat ttatttacca tacatccaga
    17821 aagtagttaa ccatatttgg gttatgaatg aagtagaaac aaatgcgatt tcaggaatta
    17881 tcatgcaaac attcctaaga aaaaaactcc taatctataa ggaggttatg cagacccttg
    17941 accagtaccg cgatgtctgg caggataatg aagatttttg taaggctgtc gaccgtttta
    18001 gtgtggtgtc acagagggaa agcctagacc tgtatctggc tgataatatc ctcaaataca
    18061 ccattgactt tagaatgttc ggattccgga aaaacaacct tggttttttt caacagtatt
    18121 tccgcgcaag agtattggcc aaaagagagc tggttaagta gtttaagttt catggattag
    18181 gttagggaga gtcctggtga ttgccgggac ttttctattt tagtgtttca gcaaaaccgg
    18241 tgttaaccgc ctataatgcc ggccccctga cagatttgat tttcagctca taaaattccg
    18301 gggttatgaa ccgcatgtaa actgaggtta agaatttctg caatttcttt gaagagtgac
    18361 cttggattac tttttttcac tttcaatttc tcttactata agttccgctg gagaaccatt
    18421 tcttcttcac caggccattt ggccaggata atatcagaaa ttttattaaa gaaaaaattt
    18481 tctccttttg aatttatccg tgaatcagtt tattaattgc tcgccccaaa cgatcaggaa
    18541 tccatcaggt ctcaaaaaaa attgctgaaa agaagccaaa ctcaaaataa agagtttaag
    18601 gaatagggtt aatgttaaat ttcccccgag tcggcagatg aaattatttt tctgatattt
    18661 acgaagccat ataccattgc gcccagccca accgcc
//
LOCUS       DASWHX010000025         6698 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_2232755, whole genome shotgun sequence.
ACCESSION   DASWHX010000025 DASWHX010000000
VERSION     DASWHX010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 6698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6698
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2232755"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..505
                     /locus_tag="VGA21_01370"
     CDS             <1..505
                     /locus_tag="VGA21_01370"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX9649183.1"
                     /translation="NYQEIISGLEKVWKTFESVRPFTPFFLNERLNNLYEDEKTTGDM
                     IGYFSLLAIFIACMGLLGLATYSTQVRVKEIGIRKVLGASSGNLIGLISRDFSLLVFI
                     AMLLSWPVAWYLMNEWLSTFTYRVNMSLGIFVISGIGTLLLALMVISINAFKAATANP
                     VDSLRNE"
     gene            662..928
                     /locus_tag="VGA21_01375"
     CDS             662..928
                     /locus_tag="VGA21_01375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649184.1"
                     /translation="MSAANIYLTFDGNCEDVFNFYKSIFGGEFLSVNRFKEVPQDQGM
                     EIGKEDEEKIMHITLPIGKDSVLMGSDTAGGYGGPIVQGNNFSI"
     gene            929..1099
                     /locus_tag="VGA21_01380"
     CDS             929..1099
                     /locus_tag="VGA21_01380"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX9649185.1"
                     /translation="MNADSKEDADKFFTALSAGGAVKMPMGDTFWGSYFGMLTDKFGI
                     NWMISFDHGQRE"
     gene            complement(1171..1785)
                     /locus_tag="VGA21_01385"
     CDS             complement(1171..1785)
                     /locus_tag="VGA21_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008199074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate-dependent dioxygenase AlkB"
                     /protein_id="HEX9649186.1"
                     /translation="MDLFNSKKLRNLLHHDGVVHYYGKIYSGNEAQYYFERMMKDIEW
                     KNDEAIIFGRHIITKRKAAWYGDHEYAYTYSNATKVALPWTKELLELKQRVESFTKSS
                     YNSCLLNLYHTGDEGMAWHSDDEKALAKDSSIASLTFGAERNFLFRHKESKQKISIIL
                     ESGSLLVMKGETQTYWQHRLPPTKKVRRPRVNLTFRTMQMEVRV"
     gene            complement(1772..2017)
                     /locus_tag="VGA21_01390"
     CDS             complement(1772..2017)
                     /locus_tag="VGA21_01390"
                     /inference="COORDINATES: protein motif:HMM:NF014821.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ada metal-binding domain-containing protein"
                     /protein_id="HEX9649187.1"
                     /translation="MIHHSDITTFSLTSRIKNKIIRLAGNKQLKIYGKLNCKSGGRML
                     IKNRVFFQSENEAWENGFRPCGNCLRTQYLNWKNGSF"
     gene            complement(2026..2862)
                     /locus_tag="VGA21_01395"
     CDS             complement(2026..2862)
                     /locus_tag="VGA21_01395"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020531998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEX9649188.1"
                     /translation="MKEQEINYQRIAAAITFINENFKKQPTLDEVAEKVHLSPHHFQK
                     IFKEWAGVSPKKFLQYLSIEYAKDILKEKQATLFDAAYETGLSGTGRLHDLFVNIEGM
                     TPGEFKNGGENLIINYSFAESPFGNILVGSTSKGICYMAFADDEKKSFMDLTNKFPKA
                     QMKQITDIMQQNALYIFRHDWSKLGEIKLHLKGTDFQLKVWEALLKIPIGQLSTYGLV
                     AKAIQKPEASRAVGTAIGSNPVAYLIPCHRVIQSTGNFGGYMWGATRKAAIIGWEAAK
                     MN"
     gene            3260..3475
                     /locus_tag="VGA21_01400"
     CDS             3260..3475
                     /locus_tag="VGA21_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018343407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX9649189.1"
                     /translation="MTKDELKKKIGRRVVELRKQKGWSQSDLARACNKDRQAMEKLEN
                     GRVNPTLYSLLELSEVMGVSLPHLVKF"
     gene            complement(3636..4640)
                     /locus_tag="VGA21_01405"
     CDS             complement(3636..4640)
                     /locus_tag="VGA21_01405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4405 domain-containing protein"
                     /protein_id="HEX9649190.1"
                     /translation="MPKRPFVSLFIATTFIVLSVSGILLFTVKASGLLIFLHVFFGGL
                     FLSGALLHFASNWISLKKYLRRKWVVPISILLLLGLFGFEVFPVDFLANSYARFKSKQ
                     PQTFDSNIFVEYPLHSNSNLTLEVRAGQHFWYPQIVVWIENNEGEYLETLFTTYSTAK
                     GVFYGGRTKENFKDLDSGLNTKENIIKVDALPYWSHKRGIETAGGYFSPNGDNQLPDG
                     ITGATPGNSFNLRTKYPELTTKFKILLEANVAFDENEFFSEFDFPDDSLYHSGAGLLG
                     QPSIIYEAVIEISSENKYYLMNYKGHTFPSGNSGEIIQDKAGLTTALEIIDLALIKVN
                     "
     gene            complement(4668..5063)
                     /locus_tag="VGA21_01410"
     CDS             complement(4668..5063)
                     /locus_tag="VGA21_01410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649191.1"
                     /translation="MKKLVLISLLGIGISSSLFGQEQIGQEQKSGCSPQTCGPENTKI
                     GEAVILTELRKDLINLKDLVKVNKGLIEVTEVAIGTDDDESLQIMVKEIAKLNDLLNL
                     KTVNYTGTNAEIVRHLRNNIINLKNSLNK"
     gene            complement(5136..5666)
                     /locus_tag="VGA21_01415"
     CDS             complement(5136..5666)
                     /locus_tag="VGA21_01415"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX9649192.1"
                     /translation="MEFLTFYDKVFQYFSKKVPTREDALDLTQGVFEKVVANLPTVKK
                     VNNVSHWIFKVSKNHLIDYYRRKSIQSKFTTSDFHQSADDTEFQNEIIKCQDHYMNQL
                     DDETKYLLIESEIKGKSQKALAEEFGVSYSTIRSKIQRGRTKIRSIFLEKCYLEYDSA
                     GRVTCCVDKDPEKKSC"
     gene            5821..6195
                     /locus_tag="VGA21_01420"
     CDS             5821..6195
                     /locus_tag="VGA21_01420"
                     /inference="COORDINATES: protein motif:HMM:NF042518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6567 family protein"
                     /protein_id="HEX9649193.1"
                     /translation="MRKELTTLVLGLTLIIFGTSCGSNLGFLSDKNRTVTNIELGSKN
                     FTVLGRVSGTSSATYYLGFGGMKNQDLLELAMKDLLSKANLEGGPRALANQIVDVHVG
                     GFPLLYAKITVKISAHVIEFTD"
BASE COUNT         1979 a         1288 c         1279 g         2152 t
ORIGIN      
        1 taactatcag gaaatcattt ccggacttga aaaagtctgg aaaacctttg agtcagtaag
       61 acctttcact ccctttttct taaatgagcg gttgaacaac ctttatgaag atgaaaaaac
      121 taccggagac atgattgggt atttctcgtt gctggcaatt tttattgcct gtatgggatt
      181 attaggattg gcgacttact ctacacaagt tagagttaaa gaaattggaa ttagaaaagt
      241 tctgggagca agttctggta atctgatcgg cttgataagc cgtgatttta gtttactggt
      301 ttttattgcc atgctgctat cctggcccgt tgcgtggtat ttaatgaatg aatggctgag
      361 tacatttacg tatcgggtca acatgagttt aggaatattt gtgatttcag gaattggaac
      421 gctgttattg gcattaatgg ttatttctat aaatgcattt aaagccgcca ccgcaaaccc
      481 ggttgattcg ctgagaaatg aataacagct gtcattaaat gtcatgccga gtttagagtt
      541 tagagtttag agtttagagt tgagagttaa gagttgagag taaacgcttt tgaaagattt
      601 aggaaatatc aaacttaact tctggttttc gtgttcaatg ttcacttaaa gagaaaatac
      661 tatgtcagca gctaatatct atctcacatt tgatggtaat tgtgaagatg tattcaattt
      721 ttacaaatca atttttggtg gtgaatttct ttctgtgaat cgatttaaag aggttcccca
      781 ggatcagggg atggaaatag gaaaagaaga tgaagaaaaa attatgcata taactttacc
      841 cattggcaaa gacagtgttc ttatgggcag cgatacggcg gggggatatg gggggccaat
      901 agtgcagggc aataatttct caatttaggt gaatgcagat agtaaggaag atgcggataa
      961 attctttact gcactgtccg cagggggcgc agttaagatg cccatgggcg acacattttg
     1021 gggatcctat ttcggcatgc taactgacaa gtttggtatt aactggatga tcagtttcga
     1081 tcatggtcag agggaatagc ttaccaacta aactcctaac tctaaactct caactcttaa
     1141 ctctcaactc ctaactccta actcctaact ctaaactcta acttccatct gcatcgtccg
     1201 gaaagttaga ttcaccctcg gtcgcctgac ttttttagtg ggaggcagcc ggtgctgcca
     1261 ataggtttga gtttctccct tcatcaccag caaacttccg ctctcaagta tgatggatat
     1321 tttttgtttg ctttccttat gccggaataa aaaattgcgc tcagcaccaa atgtcaggga
     1381 agcaatggat gaatctttgg ccaaagcttt ttcatcatcg ctatgccagg ccataccctc
     1441 atcaccagta tgatagaggt taagcaaaca ggaattgtag ctggatttag tgaagctctc
     1501 tactctctgc tttagttcca gtaattcttt cgtccatggc agagccactt tggtagcgtt
     1561 tgagtaagta tacgcatact catggtctcc ataccaggcc gcttttcttt tggtgataat
     1621 atgccttccg aaaataatgg cttcatcatt tttccattcg atatccttca tcatacgctc
     1681 aaaataatat tgcgcttcat ttccggaata aattttcccg taataatgca cgacaccatc
     1741 atgatggagc agatttcgta actttttgct attaaaaaga tccatttttc caattcaaat
     1801 attgagttct caaacaattt ccacagggtc taaatccatt ttcccatgct tcattttctg
     1861 attgaaaaaa aacccggttt tttattaaca tccgcccgcc ggatttacaa ttaagtttgc
     1921 cataaatttt caactgttta tttcctgcaa gccgaattat tttattcttg attctcgaag
     1981 tcagggagaa tgtggttatg tcactatgat gaatcattta taaaatcagt tcatttttgc
     2041 agcttcccat ccaataatag ctgccttgcg ggtagctccc cacatgtaac ctccgaaatt
     2101 tccagtcgac tgaattaccc ggtgacaggg aataagataa gcaaccgggt tgcttccaat
     2161 tgcggttcct acggctcgtg aagcttcagg tttttgaata gctttcgcca ctagtccata
     2221 agttgaaagc tggccaatcg gaattttcag caatgcttcc caaactttaa gctgaaagtc
     2281 agttcctttg agatggagtt ttatttcgcc cagcttactc cagtcatgcc ggaaaatata
     2341 caaagcattt tgctgcatga tatccgtaat ctgtttcatc tgtgctttgg gaaacttgtt
     2401 ggttaaatcc atgaatgatt tcttctcatc atcggcaaaa gccatatagc aaataccttt
     2461 tgaagtggag ccaaccagaa tattgccaaa cggactttct gcaaaactgt aattgataat
     2521 caaattttca ccaccgttct taaattcgcc gggcgtcatt ccttcgatat ttacaaacag
     2581 atcgtgcaat ctgcccgttc ctgaaaggcc ggtttcatag gccgcgtcaa acaaggttgc
     2641 ctgcttttcc tttaagatat ccttagcata ctcaatgctc aggtattgca gaaatttttt
     2701 aggactcaca ccggcccatt ccttaaatat tttttgaaaa tggtgtgggc taagatgcac
     2761 tttttcagct acttcatcca gcgtgggctg tttcttgaaa ttctcgttga taaaggttat
     2821 ggcggcggct atgcgctgat aatttatctc ctgctctttc attttgatta taatttacaa
     2881 caaaagtatt ttggaaagaa aggggatgaa acccgatagt tgcgaagata tatgaagata
     2941 tatatgtaga gttttattta aagtacacca ttaatcaggt tttttttcac ttaataaggt
     3001 gataaataac ctgtttttgt gacaacagat ttgaatttga tctccgacat taaaacctaa
     3061 actctccaac caacggccag agagcttgat ttcaggaaca attattttgc tcttttcgaa
     3121 gccacggaat tttgaataaa ttcttagttt tctgttattg atcaaaatta aaatttgttg
     3181 catgcaagct acaaaagcaa gatgaattgt caaattataa tatgacacaa ttgtcgtata
     3241 ataaaatgac atttaggatg tgacaaaaga tgagcttaaa aagaaaatcg gccggcgtgt
     3301 agttgagcta agaaagcaga aaggttggag ccaatccgac ctggcaaggg cctgcaataa
     3361 agaccgccag gccatggaaa aactggaaaa tggaagagta aaccccacac tttattcatt
     3421 gctcgaactc tccgaagtaa tgggcgtaag tttaccacat ttggttaagt tctaaaattt
     3481 taatgatcgc tttacattta ttcaattctt tttttctgga gactcctgcg aaattggatt
     3541 gtggcatttt tcaacttagg aaaattggta gttgttaacc atgcatcaga tatttggatc
     3601 tgaattaact cacgttcatg atttatatat tttttttagt ttactttgat taaggctaaa
     3661 tcgattattt caagagcagt ggttaagcct gctttatcct gaattatctc tccgctgttg
     3721 ccggatggaa atgtgtgacc cttgtaattc atcagataat atttgttttc actactaatt
     3781 tctataactg cttcgtaaat tatggaaggc tgacctaata aacctgctcc actatggtaa
     3841 agggaatcat ctggaaaatc aaattctgag aaaaactcat tttcgtcaaa agccacattc
     3901 gcctctaata ggattttgaa ttttgttgtc aactcaggat acttagtccg gagattaaag
     3961 gaattgccag gcgttgctcc ggtgattcca tcaggcaact gattatcacc attaggtgag
     4021 aaatatccgc cggcagtttc aatccctcgt ttatgcgacc aatatggaag ggcgtctacc
     4081 ttaataatat tttctttagt gtttaacccg gaatctaaat ccttgaaatt ctcttttgtt
     4141 ctgcctccat aaaagactcc ttttgcagtt gaataggttg tgaaaagtgt ttccagatat
     4201 tctccttcgt tgttctcaat ccaaaccact atctgcggat accaaaaatg ctgaccggcc
     4261 ctcacctcaa gggttaagtt gctattcgaa tgcaaagggt actccacaaa aatattgcta
     4321 tcgaaagtct gtggttgttt tgatttaaat cgggcataag aatttgcaag gaaatcaaca
     4381 ggaaaaacct cgaaaccgaa aagccccagt aatagaagaa tactaatagg aacaacccat
     4441 tttcttctca ggtatttttt taatgaaatc caatttgaag caaaatgcag gagtgcacct
     4501 gaaagaaata agcctccaaa aaaaacatgc aggaagatca gaagtccact agccttaaca
     4561 gtgaaaagca agattccgct tacgcttaga acaatgaatg tagtagctat gaaaaggctt
     4621 acaaacggtc tttttggcat taaattgaaa cccaagattc cctggcgtta cttattcagg
     4681 gaattcttga ggttaataat attattcctg agatgtctga ctatttcggc attggtgcct
     4741 gtgtaattaa ccgtcttgag gttcaataaa tcattgagct ttgcaatttc ttttaccatt
     4801 atctggaggc tttcatcatc atcagtacct atggctactt ctgtaacttc aataagtcct
     4861 ttattgactt tcaccaggtc ttttaaatta atcaaatctt tgcgtagctc agtcaaaatt
     4921 acagcttcac ctatttttgt attttcagga ccgcaagtct gaggcgaaca accgcttttt
     4981 tgctcctgac cgatttgctc ctgaccgaac aatgaggaag aaattcctat cccaagcaag
     5041 gatatcaata ctaacttttt cataatttat tcaaatttaa atttaatgtg acttattcac
     5101 agagtaagac gttcaaccaa caaaaagatg caggattaac aagatttctt ttccgggtct
     5161 ttgtcgacgc agcaagtaac tcttcctgct gagtcatatt ctaaataaca cttttcaagg
     5221 aatattgacc ggattttggt gcgccctctt tgaattttgg acctaatggt ggagtaactc
     5281 actccaaatt cttcagccaa agctttttgt gattttccct taatttcaga ctcgattaac
     5341 aagtattttg tttcatcatc cagttgattc atgtaatgat cctgacactt tattatttca
     5401 ttttgaaact ctgtatcgtc agcactttga tgaaaatccg aagtagtaaa ttttgactga
     5461 atgctcttcc ttctataata atcgattaaa tgattttttg aaaccttgaa tatccagtga
     5521 cttacgttat tgaccttttt tacagttgga agatttgcaa caactttttc aaaaactccc
     5581 tgtgtaagat caagagcatc ttctctggtt ggaactttct tactaaaata ctgaaagacc
     5641 ttatcgtaaa aagttaaaaa ctccatgcat gaaactatat ggcagcagaa ccaggttggt
     5701 atccgaaaat tacgaaaaca aaatctttac gttgcatgtt ctgaaattaa tattatgatt
     5761 ctcctcctat tttatcattt tctaatgaat taccttagtt aaaagctgac aaataaaaat
     5821 atgagaaagg aattaacaac tttagtttta ggactgacgc ttataatttt tgggacaagc
     5881 tgtggctcta atcttggttt cttgtcagat aaaaaccgga cggtaaccaa tatagaactg
     5941 ggctcaaaaa atttcacagt actaggcagg gtcagtggca catcatcagc tacttattat
     6001 ttgggttttg gtggaatgaa gaaccaggat ctgttggaat tagcaatgaa ggatctcctt
     6061 tccaaagcca atttggaagg tggaccgcgt gcgctcgcca atcagatcgt ggatgttcac
     6121 gtaggaggat ttccgctgct ttatgcgaaa ataactgtga agataagcgc ccatgtaatt
     6181 gagtttaccg attaatattt tcaatcagcg ggatcaagga aaatcttctc ccgcagatta
     6241 cctttagtat tgcgcagaaa tctttgaata attttctctg catgatctgc gattatcagc
     6301 gggattaagt aaaattttct cccgcagatt acgcggattt gcgcagaaat ctttgaatga
     6361 tttttttctg cggtgatctg cgatatcagc gggatcaagt aaaatcttct cccgcagatt
     6421 gcgtggattt gcgcacagaa atccctgaat aaattttctc tgcggtgatc cgcgatacca
     6481 gtgggattaa taaaaatctt ctcctgcaga ttgcaacgga tttgcgcaga aatctctgaa
     6541 tgatttttct ctgcggcgat cgacaatatc agtaggatca agtaaaatct tccccacaga
     6601 ttgcgcggat ttgcgcagaa atccttgaat aatttttctg cggtgatctg cgatatcagc
     6661 gggattaatg atttacagaa actctgttta cataacca
//
LOCUS       DASWHX010000026         3159 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_1124354, whole genome shotgun sequence.
ACCESSION   DASWHX010000026 DASWHX010000000
VERSION     DASWHX010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 3159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3159
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1124354"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..801)
                     /locus_tag="VGA21_01425"
     CDS             complement(<1..801)
                     /locus_tag="VGA21_01425"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX9649194.1"
                     /translation="MLTPQGKPFIYLDNAATTQKPAAVLNAIDSFYRGRYSNVHRGAY
                     WPASQTNLLFEKSREKVAGFLNAGNPECIVFTGGTTDSINKVANALAGLLNENDEILI
                     SEMEHHSNLVPWQILTKKNKLFLSFIEIDKNGKWKLSEVINKISERTKVIAVSAISNS
                     LGTINDVESVLKSSNPDYTITLVDAAQSVANYQTDVKALSCGFLTFSAHKMYGPMGVG
                     VLYGKQNLLEKLPPLAYGGGMITEVEMHSTLFRNSPHKHEAGTPNVAGV"
     gene            complement(860..1456)
                     /locus_tag="VGA21_01430"
     CDS             complement(860..1456)
                     /locus_tag="VGA21_01430"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEX9649195.1"
                     /translation="MISAIFLAAGLSSRMEGENKLLMTLNGKTILQLTLESILASEVM
                     EVIVVVGHQQENVRETIERNKYPVRIVENGDYKSGMTSSIQAGIRHASEFCEGYIICM
                     GDMPLLETRHYNSVILGFRKSFIFQKDCIIIPFFDGRRGNPVIFSSAYKSEILAHKKP
                     EGLKEVIEKYPEKVSKIPMETKIRILDIDKKEDYLAMI"
     gene            1592..2047
                     /locus_tag="VGA21_01435"
     CDS             1592..2047
                     /locus_tag="VGA21_01435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649196.1"
                     /translation="MNHYNAKQLKKISKLYAFNCLKRETFSFLAYGTLREGDYNHNYY
                     GAGLKSTNKKSFVDNSSLWTHGPYPYLLKEEFQIRQVLVEEIEFANQSTAFEIIEMEI
                     ASGYQLGLIEKKLIFFWPDNSQTLSAFLNRPVIEISSGDWFDYHSATSL"
     gene            complement(2030..2884)
                     /locus_tag="VGA21_01440"
     CDS             complement(2030..2884)
                     /locus_tag="VGA21_01440"
                     /inference="COORDINATES: protein motif:HMM:NF022588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HEX9649197.1"
                     /translation="MKRILLLITLVNYNSFSQDLNFPTLSPASKISQEIGLTEINLSY
                     ARPGAKGREIFGGLVPYGEIWRTGANASTKLTFTEDVKVGGNDLPAGTYALYTIPNEK
                     TWTIISHKNIRMRSLAGNVYKQENDAFRFEVPSINNPMTVETFTIQFTDITTESCNLT
                     LSWANTMVKIPIQVSVDTKIAARLEELMKNSEQINARTYFRSAEYFYHNDKDLNKALE
                     WSNKSLELSPKNPMFGLLKAKILSDLGKTDESIQTITEAHQWAVDSNNSNYEEQTRLF
                     MEGLKRGG"
BASE COUNT          893 a          651 c          587 g         1028 t
ORIGIN      
        1 tactccggct acattgggag tgccggcttc atgtttgtgc ggcgagtttc taaaaagcgt
       61 tgagtgcatt tcaacctctg ttatcattcc gccgccgtat gctaatgggg gtaatttctc
      121 aagcagattt tgctttccat aaagaacacc aacgcccata ggtccataca tcttatgcgc
      181 agagaatgtc agaaaaccac acgacagagc cttgacatca gtttggtaat tagccactga
      241 ctgggcggca tcaaccagtg taattgtata atcgggattc gacgatttta aaacggattc
      301 cacatcgtta atggtaccca gtgaattgga tattgcactt acagcaatga ctttggttct
      361 ttcactgatc ttatttatta cttcactcaa tttccatttt ccattcttat cgatttcaat
      421 aaagcttaag aataacttgt ttttcttagt caggatttgc catggcacca aattggaatg
      481 gtgttccatt tcactaatca ggatttcatc attttcattt agtaaaccgg caagagcgtt
      541 agctacttta ttaattgaat cagttgtgcc gccggtaaaa acaatacact ccggatttcc
      601 ggcatttaga aatcctgcaa ccttctcacg acttttttca aaaagaagat ttgtttggct
      661 tgccggccag taggctcccc gatgaacatt tgaataccgg ccgcggtaaa aactgtcgat
      721 agcatttaaa actgctgccg gcttctgagt cgtagctgca ttatccagat aaataaacgg
      781 ttttccctga ggagtcagca tggacaaaat ggggaaatct gagcgaattt tttgctgata
      841 agtcacagat taattcacct taaatcatag ccaaataatc ttctttctta tcaatatcca
      901 gaattcggat tttcgtctcc attggaattt tagaaacttt ttccgggtac ttttcaatta
      961 cctcttttaa tccttccggt tttttgtgtg ccaatatttc tgatttgtat gctgatgaaa
     1021 aaataaccgg attgcctctt ctgccatcaa agaatggaat tattatacaa tccttttgaa
     1081 aaataaatga ctttcgaaac cctaaaatta cggagttgta atgtctggtt tccagcaaag
     1141 gcatatcacc catgcaaatt atgtaaccct cgcaaaactc cgatgcatgc cggattccag
     1201 cctgtatgga cgacgtcatt ccggatttat aatctccatt ctcaacaatt ctgaccggat
     1261 atttatttct ttctattgtt tcccgcacat tttcctgctg gtgaccgaca actacaatca
     1321 cctccattac ttcactggcc agaatggatt ccagtgttaa ctgcagaatc gttttgccgt
     1381 tcagtgtcat aaggagcttg ttttctccct ccattcttga ggataatccg gccgcgagga
     1441 atatggcgct aatcatttgt aaattattat gtattaaatt tagtaaatcc cttctggctt
     1501 aaaaaattct taattaggaa tctgcaattt tctgttaaca attttgcaaa tactgaatta
     1561 acaatagatt tgagcaacca cagggaaaat tgtgaaccat tacaacgcta aacagcttaa
     1621 gaagatttcc aagctctatg cattcaactg cttaaaaagg gaaacttttt cttttcttgc
     1681 ttatggtacc ttaagggagg gtgactataa tcataattat tatggggcag gtctcaagtc
     1741 aacaaataag aaatcatttg tcgacaatag ttctttgtgg acccatggcc cttatcccta
     1801 tttgctaaaa gaggaatttc aaatcagaca agttttggtt gaagaaatag aatttgcaaa
     1861 ccaaagcaca gccttcgaaa tcattgagat ggaaattgcc tcaggttatc agcttggttt
     1921 aatagaaaag aaattaattt ttttctggcc tgacaactca caaacattat ccgctttcct
     1981 taaccggccg gtaatcgaaa tctcttcagg ggattggttt gattaccatt cagccacctc
     2041 gctttagacc ttccatgaat aaccttgtct gttcttcata atttgaattg ttggaatcaa
     2101 ctgcccattg atgtgcttcg gtaatggttt gaattgattc atctgttttc cctaagtcac
     2161 ttaatatttt tgctttaagc agtccgaaca tcgggttttt cggactcaac tccagggatt
     2221 tgtttgacca ttccaatgcc ttgttcaaat ccttatcatt gtggtaaaaa tactcggctg
     2281 acctgaagta agtcctcgcg tttatttgct ctgaattctt catcagttct tccagccggg
     2341 ccgcaatttt agtatcaaca cttacctgaa tgggtatctt taccatagtg ttagcccaac
     2401 tcaatgttaa attgcagctt tcggtagtaa tatcagtaaa ttgaatagtg aaagtctcca
     2461 ctgtcattgg attattaatc gatggaactt caaagcggaa tgcatcattc tcctgcttgt
     2521 aaacattacc tgccagggat cgcattctta tattcttgtg actaattatg gtccatgttt
     2581 tctcattggg tattgtatat agcgcgtagg ttccggcggg caaatcattc ccgcctacct
     2641 ttacatcttc tgtaaaagtt agcttcgttg aagcgttcgc gccagtgcgc caaatctccc
     2701 cgtagggaac cagaccgcca aaaatttctc ttccctttgc accagggcgt gcataggata
     2761 aatttatctc agttaaacca atctcctgcg agatttttga ggccggactg agcgtgggaa
     2821 aattcagatc ctgagaaaat gaattataat taactaaagt gattaatagt aatattcttt
     2881 tcatagctta aaatattaat tgggagaatg aagataataa aaattgggtg acctttcaat
     2941 gttaaattga cgcagtgctt cggttgcctt gcacactcgt cttctgttgt gtacatagaa
     3001 atcccgagtc ttgtagaggg aatttgctcc tcgctttgaa ggttttagac ccggcgtttc
     3061 atgaccaggt aggaaggcaa aacaactttt aaaaaggtta aaaatccttt agattagttg
     3121 actatataaa aggggtatat tcaatagaca tatccggtt
//
LOCUS       DASWHX010000027        11758 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_386849, whole genome shotgun sequence.
ACCESSION   DASWHX010000027 DASWHX010000000
VERSION     DASWHX010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 11758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11758
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_386849"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..975)
                     /gene="dnaG"
                     /locus_tag="VGA21_01445"
     CDS             complement(<1..975)
                     /gene="dnaG"
                     /locus_tag="VGA21_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009583176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEX9649198.1"
                     /translation="MIDRRIIEEIKSRIDIVEVIGDFVTLKKSGQSYKALSPFSTEKT
                     PSFFVSPSKGIYKDFSSGKGGDAINFIMEHDGLSYIEAIKYLAKKYGIEVQEKELTDE
                     DIKEQNERESLFIVLNFANKWFINNLHETDEGRSVGLGYFRERGYKDETIKTFELGYS
                     IEEWDAFYQEAKANKYSEDILEKAGLIIRKDRKTYDRFRGRVIFPIHNLSGKVIAFGG
                     RTLKKDKNLPKYVNSPETEVYHKSSVLYGLNLSKKSIRDLNLCYLVEGYTDVIALYQA
                     GIGNVVSSSGTALTKEQITLIKRYTENVTILYDGDSAGLKASLRGLDML"
     gene            1094..2194
                     /locus_tag="VGA21_01450"
     CDS             1094..2194
                     /locus_tag="VGA21_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015267148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140663 - ATP-dependent FeS chaperone
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HEX9649199.1"
                     /translation="MSATKEEILKALSTVEDPDLKKDLVTLNMIQNLVVDGNRISFKV
                     VLTTPACPLKEMIKNACEEAIHSIVNSNADIAIEMASSVTTIRLDNTPLLPNVKNIVA
                     VASGKGGVGKSTVTANLAAALAKQGAKVGLLDADIYGPSIPTMFNCEHEKPGMREVNG
                     KNMIVPLEQYGVKLISIGFLTPADNAVVWRGPMASSAFKQFISDVLWGDLDYLFIDLP
                     PGTSDIHLTLVQTVPVTGVIIVTTPQKVALADATKGLSMFRQPQINVPILGVVENMAF
                     FTPEELPDSKYYIFGKEGGKLLAEKHGVPFLGEIPLIQGIRESGDNGQPMSIKEDMTR
                     TYFAKLAENVAQQIAIRNAKLEKTKTVVVNQN"
     gene            2199..2495
                     /locus_tag="VGA21_01455"
     CDS             2199..2495
                     /locus_tag="VGA21_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020532285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HEX9649200.1"
                     /translation="MKKLTPSLKKRIEEALNSIRPYLEADGGDARILDLNDEMVLTIE
                     FVGSCGTCPMSTMTLKAGVEESIRRLVPEIKAVDCVNLTSPNDPRAQLPQNMVR"
     gene            2575..5553
                     /locus_tag="VGA21_01460"
     CDS             2575..5553
                     /locus_tag="VGA21_01460"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor J domain-containing protein"
                     /protein_id="HEX9649201.1"
                     /translation="MLSDSNSHAQERCGIIEYQEYLKSKGIIIETNSEFEDWLIKKKN
                     ERNLNQKLGVEKITQDIYTIPIVVHVIHNDEPIGSGANISDQQIQSQIEVLNEDFRRQ
                     NLDAILTLAEFVPVASDPEIEFVLAKQDPDGLATNGITRSKGSQASYTFADAELLKNQ
                     SYWPSEDYLNIWVANLSPGSLGLASFPVTDLEGIENPELNRLYDGVVVAYDVFGSQDK
                     DPNADLLNNFDKGRTTTHEIGHFLGLRHISGDGNCSVDDFCLDTPNQNGQTNGCPASR
                     NTCGSNDMYQNYLDYTDDRCMNIFTMCQKDRMRTVLENSPRRLSLLTSPGLEEPVPIN
                     ADIAISEIHAPSTGECTQLITPSLQLRNAGTDVITEAIIKVSANNIELETSTFNLNLA
                     SAASTDVTFNQIQTQSKGYFTFDFEVQSVNGVPDLNTLNNNKSINTFISFDLSLPTIE
                     TFDAFPVSWNLINPDESITWELSNAPSISSSNTAAKINFYDYENGLGLEDYLITPLID
                     LSGSTTASLIFDRAYAIYPGSESDNLTIAVSLDCGTTFPFKVYNRNSISLQTATPTPL
                     PFIPTSANEWQKDIVDLSAFAGLTDLQIAFIATNGFGNNLYLDNIKIIKSPIADISIS
                     RVTSPDEISCNISVSPTLLVTNSGTVTINNLSASIKSNGVEISTKDFGSLNLTAGNQR
                     TLILDPINLEPGNNQLTFEVKLLAGTTDFNPDDNALDGNFVLVTESEFIPLRQKFKEP
                     GNIGNWIIANSGAGEQSWKVEEAFSQSQNNYAASVKAWSNQAVGSRSVLISPVLNFFL
                     VRESSMFFRVSYASRLDKNDILRIKASTDCGNNFEVIYEKGSQDLGIRQSESEWYPQS
                     ELDWINEYVDLTNFAGEQQVIFAFEFTDGGGNNLFLDDIEFFISNDPNPVLINSTWSV
                     YPNPAVNGIFDITLNFDEKQKSEVRIINSTGQEMYRTTVINSLNQTVPVDLTTHPSGI
                     YFVRLISKEVNETRTVIIKP"
     gene            5603..6673
                     /locus_tag="VGA21_01465"
     CDS             5603..6673
                     /locus_tag="VGA21_01465"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF002528.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HEX9649202.1"
                     /translation="MHKTNIAIIYGGRSVEHQISIRSARNIAENINRELFHPVLIGIT
                     MEGNWFLTDTVSREISEGLALKLNLNIEAKELIDASGISHAIDIAFPVLHGTDGEDGS
                     VQGLLKVLNIPCVGSDVLGSAISMNKLVSKQLLKHAGIPVGNFIGIYKDRHYDVNKII
                     TDLGIPLVVKPSSLGSSVGVGIAKRADQVESMIQDAFQYDYSIILEEYIQGREIECAI
                     IGNENPQASLPGEIVVSEKYEFYSYDAKYVDPFAARLDIPAKMKPEIQKKIQQTCLEA
                     YRTLNCCDYARVDLFLTKKGEIFINEINTIPGFTNSSMFPKMWEHMGIPYSEIITKLI
                     ELAMGRQKKGNELNRIYNSNLE"
     gene            6673..7461
                     /locus_tag="VGA21_01470"
     CDS             6673..7461
                     /locus_tag="VGA21_01470"
                     /inference="COORDINATES: protein motif:HMM:NF015734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HEX9649203.1"
                     /translation="MNWFQTKTWVDLVRHLFLIFVIVTVLVLGLFYIYLPAKTNHGEA
                     ITVPDLIGLNISDLDKFLVERNLRYEINDSSYDHVAEPLTVLQQFPKPGVLVKEDRKI
                     YLTINRKTPPEIRLPASIIGYSLKNAEAVLTSYELLRGKITYVPYPYSNLVLEMKLQG
                     KNLYEGNRVPKGSAIDLVVGDGYGKRKFAIDDLTRLTLDDAKIYLNGSGLKLGIVTGQ
                     KDSTDTNWIVSKQIPLPNDMVSIGQFVDIWVAPLDSLNNEPLDE"
     gene            7442..9358
                     /locus_tag="VGA21_01475"
     CDS             7442..9358
                     /locus_tag="VGA21_01475"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEX9649204.1"
                     /translation="MSLWMNSWRMMTMKSSSLFLLIVTFLVQAHAQIKVVPIQGVTHS
                     KKNNAYSTQNYRITAEKLVLPLWEDFSTYEGQPDTTLWINSANVWVNHGMCINCPSIN
                     VATFDGLDKNGNPYSLDPSDIGLTDSLVSKPIDLTQVAFAKRNSVYLSFYYQKGGQGE
                     PPDAQDMIELQFRTAGNRWARAWPADPESLNSLTSIFNGVSIQIVDSLFHDNFQFRFL
                     STGRKTGSFDVWNIDYIYLDKNRSQDDYEILDRAITSIPNSIFKNYTAIPLRQIYFGD
                     QSIIDSTEVRLFNYEVSEPVQGIEYSAILKNTKTQLEIEKIIDKKAHIIMANNFLDLK
                     AGKPTLQLLKDQVSGPDDSLFLELIYYVDTGDTLLIDEINTTTNDTTYISYIDLRVND
                     TITRKFTLHNYLAYDDGTAEFGAGINQNQGQIAYKFFIQQPDTLVAFNINFPNINGNV
                     AGLPIDLIVWSHLDGFADSELYRQPFTIQANGINELKTYELLKPLAVEDTFYIGFQQS
                     TNEFIPVGLDKNSDSGENIFYNVNGVWQANTELIGSLMMRPVFGKGGVITPINSEIPG
                     INIYPNPTDGNFTVTGNPTEVNIYDFSGRKMKATFIATSSGVAVSAIGYKQGIYLVEV
                     ITPSQRIIQKLIFR"
     gene            9381..10262
                     /gene="ppk2"
                     /locus_tag="VGA21_01480"
     CDS             9381..10262
                     /gene="ppk2"
                     /locus_tag="VGA21_01480"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002693511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HEX9649205.1"
                     /translation="MINKSDTLEQTKVYISADDFANINTQKELIKILDKKNINYSKPL
                     RILRYERELVKLQIELVKLQTWIRDTKKRVVVIFEGRDAAGKGGTIRRFREHLNPRAM
                     RVVALTNPNEVEKGQWYFRRYVKELPNPGEIVFFDRSWYNRAVVEPVNKFCSKKDYYK
                     FIQQVPEFEHMLYEDGVKIIKFWFSISKEEQLKRFESRKKNPLKQWKLSRVDERSQEL
                     WDKYTYYKEEMFSKTHTTYSPWIIVESNSKRKARLESMRYVLSNFDYPGKESARTTLY
                     PDPNTVKRFHRSVLQID"
     gene            10345..11076
                     /gene="ppk2"
                     /locus_tag="VGA21_01485"
     CDS             10345..11076
                     /gene="ppk2"
                     /locus_tag="VGA21_01485"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008624843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HEX9649206.1"
                     /translation="MRKILNYLRYEKELRELQTEAIKLQNWITNNNKRLVILYEGRDS
                     AGKGGAIRRFVEHLNPRRYKVVALPKPTIKEKGQWYFQRYAQRLPDPGEIVLFDRSWY
                     NRAIVEPVNGFCNQKQYKRHLEQIPEFEKMLKEDGTILIKLWFSISKQEQKKRLIAIK
                     KSPLKQWKLSPVDEKAQELWDVYTQYKEEMFSRTNTKENPWIVVMADSKINARVESMK
                     YILEQIPYAGKSENLEWNRNVIVQI"
     gene            complement(11073..>11758)
                     /locus_tag="VGA21_01490"
     CDS             complement(11073..>11758)
                     /locus_tag="VGA21_01490"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEX9649207.1"
                     /translation="PESELLNLLPDTIEFSTQIPKVYLGTNLIFEFFKNNVADKERLP
                     NHYEAMHYPWLFAGFLALFFTALNLIPVGQLDGGHVLYGLFGRENHRIISSGIFIAFV
                     FYAGLGVINPFDPNVGFAETVLYSILYVGFLYLTYYTISKQFRDRLFIAVLTFTAQFG
                     VVYLFPGVIGYQGWLIFAFIIGRFLGIQHPPTLYDEPISFNRKILGWIALLIFVLSFS
                     PRPIIVEGI"
BASE COUNT         3821 a         2277 c         2312 g         3348 t
ORIGIN      
        1 cagcatatcc aggcctctta acgaagcttt caaaccggca gaatcaccat catacaaaat
       61 ggtgacattc tccgtgtatc tttttattag cgttatctgc tctttggtca gagcagttcc
      121 ggaggatgac acaacattgc cgataccagc ctgataaaga gctatgacat ccgtgtaccc
      181 ttccacaaga taacagaggt tcaaatcacg gatagatttt ttacttagat tcagaccgta
      241 aagaacactg cttttgtggt aaacctctgt ctcaggacta tttacatact tgggtaaatt
      301 cttgtccttc tttaatgtcc ttcctccaaa agcaattacc ttacctgaca aattgtggat
      361 ggggaatatt accctgcctc gaaacctgtc gtacgttttt ctatcctttc tgatgatcaa
      421 tccggccttt tccagaatat cttcactgta cttattagcc tttgcttcct ggtaaaaagc
      481 atcccactcc tcgatactat aacctaattc aaaagtcttg atggtttcat ctttgtaccc
      541 tctctccctg aagtacccca gccccacgct tctcccttca tcagtctcat gaagattatt
      601 aatgaaccat ttatttgcaa aattcagaac aataaataat gactctcgtt cattttgttc
      661 cttaatatcc tcatcggtta actccttttc ctgaacttct attccatatt tcttagctaa
      721 atactttatc gcctcaatat aactaagtcc atcatgctcc atgatgaagt ttattgcgtc
      781 acctcctttt ccagagctaa agtctttgta aatgccctta gatggactaa caaaaaatga
      841 tggagttttc tctgtggaga aagggcttaa tgctttataa ctttgacctg attttttcag
      901 agtaacaaaa tcaccaataa cttctacaat atcgattctg ctctttattt cctctataat
      961 acgccggtca atcatcctta acagtaaagt tatatcgatt attacccaat ttaaactcct
     1021 aattatcaat ttagcggtaa atttattact tatccgctct ttaaaaacta aatttgctgt
     1081 tatacaaagt ggcatgagtg caacaaaaga ggaaatatta aaagcattaa gcacagttga
     1141 ggatcctgat ttaaagaagg accttgttac tcttaatatg attcagaatc tggtggttga
     1201 tggaaatcgc atttccttta aagttgtttt gactaccccg gcatgccctt tgaaagaaat
     1261 gattaagaac gcttgtgaag aagcaattca ttcaatagta aactcaaatg cggatattgc
     1321 tattgaaatg gcttcttcag taacaacaat ccgtttggat aatacccctc tccttccgaa
     1381 tgttaagaat attgttgcgg tggcttcagg caaaggtggt gtaggaaagt caaccgttac
     1441 cgcgaacctt gccgcggcac tggcaaagca gggagcaaaa gtaggattat tagatgccga
     1501 catttatggt ccatccatac ccaccatgtt taattgcgag catgaaaaac cgggtatgag
     1561 agaggttaat gggaaaaata tgattgtccc ccttgagcaa tatggcgtta agttaatttc
     1621 aattggcttt ctaaccccgg cagataacgc cgtagtgtgg cgggggccta tggcaagttc
     1681 ggctttcaaa caatttattt cagatgtctt atggggagac ctcgattacc ttttcattga
     1741 tctgccaccc ggtaccagcg atattcacct aactctagtt cagacagttc cggtgacggg
     1801 tgtaattatc gttacaacgc cccaaaaagt tgccctggcg gatgcaacca aaggtttatc
     1861 catgtttcgg cagcctcaaa ttaatgtacc tattttagga gttgtggaaa acatggcttt
     1921 tttcaccccg gaagaattac cggatagtaa atattacata tttggcaaag aaggtggcaa
     1981 gctacttgct gagaaacatg gagttccttt cttaggagaa ataccactga ttcaagggat
     2041 tcgtgaaagt ggcgataacg ggcaacctat gtcgattaaa gaagatatga ccagaacata
     2101 ctttgctaag cttgcagaaa atgttgcaca gcaaattgca ataagaaacg caaaacttga
     2161 aaaaacaaag accgtggtcg ttaatcaaaa ttgatggcat gaagaaatta acaccctcgt
     2221 tgaaaaaaag aattgaagag gcattgaact ctattcgccc gtacctggag gcggatggtg
     2281 gcgatgccag aattctggat ttgaacgatg agatggtttt aaccattgaa tttgtcggct
     2341 cttgcggaac atgtcctatg tcgacaatga cattaaaggc tggagtggag gaatcaatcc
     2401 gaagattagt acctgagatt aaagctgttg attgtgttaa cttaacttct ccaaatgacc
     2461 ctcgcgctca attgccacaa aatatggttc gctaaggcct tcgggtttct ttatcgaaag
     2521 tattttataa gaaaattatt tccacttcag attccgcttg tctcgatact ttttatgtta
     2581 tcagatagta atagtcatgc ccaggaaagg tgtggaatta ttgaatacca ggagtatctt
     2641 aaatcaaaag gtataatcat tgaaacaaat tctgaatttg aggattggct gatcaaaaag
     2701 aaaaatgaac gaaacttaaa ccaaaaactt ggggttgaaa aaatcaccca ggatatctac
     2761 acaatcccga ttgtcgtaca cgtcattcat aatgatgaac ccatcggatc cggagccaat
     2821 atttcggacc agcaaattca gagtcaaatt gaggttctga atgaagattt tcgacgacaa
     2881 aaccttgatg caatattaac acttgccgaa ttcgtgcctg ttgcgtctga tcctgaaatt
     2941 gaatttgtcc tggcaaaaca agatccggat ggcctggcaa caaatggtat aacaagatca
     3001 aaaggatcac aggctagtta tacttttgcg gatgctgaac ttttgaagaa tcaaagttat
     3061 tggccctctg aagattatct taatatttgg gtcgctaatt tatccccggg ctcccttggg
     3121 ctggcaagtt ttccggttac agacctcgaa ggaatagaaa atcccgaact aaatcgtctg
     3181 tatgatgggg ttgtggtagc atatgatgtt ttcggttctc aggacaagga tccaaacgcg
     3241 gatttactga ataattttga taaaggaaga actacaactc atgagattgg acatttcctg
     3301 ggtttaaggc acatcagtgg tgatggcaac tgctcagtag atgatttctg cctggataca
     3361 cctaaccaga atggccaaac caatggttgc ccggctagta gaaatacctg cggctcaaac
     3421 gatatgtatc aaaactattt ggactacact gacgatcgtt gcatgaatat atttacaatg
     3481 tgtcagaaag acagaatgcg aacagtgctg gaaaatagcc ccaggagact ttccctgctt
     3541 acttcacccg gactggagga acccgtgccc atcaacgcgg atattgcaat ttcagaaatc
     3601 catgctccct ctaccggcga gtgtactcaa ctaataacac cttctttgca gcttagaaat
     3661 gccggtacag atgttataac cgaagcaatt atcaaagtgt ctgccaataa tatagaactt
     3721 gaaacaagta catttaacct gaacctagcc agtgcagcat ccaccgatgt tacctttaat
     3781 caaattcaaa ctcaaagtaa gggttatttt actttcgact ttgaagttca gtccgttaat
     3841 ggtgtaccag atttaaatac actcaataat aacaagtcga taaatacctt tatctccttt
     3901 gatttatcac ttcccactat tgaaactttt gatgcatttc cagtgagttg gaatctgatt
     3961 aatcccgatg aatccataac ctgggagtta agcaacgcac cttcaatctc atcatcgaat
     4021 actgcagcca aaataaattt ctatgactat gaaaacgggc ttggacttga agattatcta
     4081 attactcccc tgatcgactt gtccggatca acaaccgctt cattgatttt tgacagggct
     4141 tacgcgatat atcccggatc cgaatctgat aaccttacca tcgctgtaag cctcgattgt
     4201 ggaactacat tcccattcaa ggtgtataac cgaaacagta tttcccttca gacagcaaca
     4261 cctactcctc ttccatttat cccgacatct gccaatgaat ggcaaaaaga tattgttgac
     4321 ttaagcgcat tcgcggggct aacggattta caaattgcct tcatagcaac taatggattt
     4381 ggcaataatc tttacctgga taacataaag ataattaagt cccccattgc ggacatcagt
     4441 attagcagag taacctcacc ggacgaaatt tcctgcaata tttcagtttc tccaacctta
     4501 ttagttacca acagcggaac tgtcacaata aacaatctaa gtgcttcaat caaatcaaac
     4561 ggagtagaaa ttagcactaa agattttggt tcattgaatc ttacagcagg gaatcaaaga
     4621 acattaattt tagatccaat aaatcttgaa ccgggaaata atcaacttac tttcgaagtt
     4681 aagcttttag caggcactac agatttcaat cctgatgaca atgccctgga tggaaatttt
     4741 gttttggtta cagaatctga atttataccc cttcgtcaga aatttaaaga accgggaaat
     4801 attggaaatt ggataattgc gaatagcggc gcgggagaac aatcatggaa agtagaagaa
     4861 gcgtttagcc agagtcagaa taattatgcc gcatcagtta aagcatggag caatcaggca
     4921 gttggttccc ggagtgtttt gatttcacca gttctgaatt ttttcctggt cagagaatca
     4981 agtatgtttt ttagagtaag ctatgcctca aggcttgaca agaatgatat cttaagaatt
     5041 aaagcttcaa cggattgcgg aaataatttt gaagtgatat atgaaaaagg aagccaggat
     5101 ttaggcatca gacagtcaga gtcagaatgg tacccgcagt cggaattgga ctggataaac
     5161 gaatacgtcg accttaccaa ttttgccgga gaacagcagg ttatattcgc ctttgagttt
     5221 accgatggtg gcggcaataa cctctttctt gatgatatcg aattctttat ttccaatgac
     5281 ccgaatccag tccttataaa cagcacctgg tctgtatatc caaaccctgc tgtaaatggt
     5341 attttcgata tcacccttaa ctttgatgaa aagcagaaaa gtgaagtaag aattattaat
     5401 tcgacaggac aggaaatgta ccgcacaact gttattaact cacttaacca aaccgtcccc
     5461 gtagatctga ccacccatcc ctccgggata tactttgtca gattgataag taaggaggta
     5521 aatgaaacaa ggacggtgat aattaagcct tgacctgtcc tttaaatatt tttatctctt
     5581 atttttggtg ctttagccga caatgcacaa gacaaacata gcaatcattt atggcggcag
     5641 gtctgtggag catcaaatat ccatcaggtc tgcaagaaat atcgctgaaa atataaacag
     5701 ggagcttttt catcctgttt taataggaat tacaatggag ggcaactggt ttctgaccga
     5761 cacagtatcc agggaaataa gtgagggact tgcattaaag ctgaatttga atattgaggc
     5821 taaagaatta atagatgcat ccggtatttc acatgcaatt gacattgctt ttccggtatt
     5881 gcatggtact gatggagaag atggcagtgt tcagggtctg ttaaaggttc tgaatattcc
     5941 ctgtgtagga agtgacgttt tgggttccgc aatttcgatg aataaactgg tatccaaaca
     6001 attattgaag catgccggaa tccccgtagg caattttata ggtatttaca aagaccgtca
     6061 ttatgatgtt aacaaaatta ttactgacct tggaattccc ctggtagtaa aaccttcatc
     6121 cctgggatca tctgttggag ttggcattgc aaaaagagcg gatcaggtgg agtccatgat
     6181 ccaagatgca tttcagtatg attattcaat tattttggaa gaatatatcc agggcaggga
     6241 aattgaatgc gcaatcattg gaaatgagaa cccacaagca tcattgccgg gtgaaattgt
     6301 agtgagcgaa aagtatgaat tttattctta tgatgcgaaa tacgttgatc ccttcgctgc
     6361 ccgtttggat atacctgcaa aaatgaaacc tgaaattcaa aaaaaaattc agcaaacttg
     6421 ccttgaagct tatcgtactc ttaattgctg tgattatgca agagtagatt tatttctaac
     6481 caaaaaaggt gaaattttca ttaatgagat taatactatt cccgggttta caaattccag
     6541 catgttccct aaaatgtggg aacatatggg tattccatat tctgaaatta tcactaaatt
     6601 aatagagctt gctatgggac gccaaaagaa aggtaacgaa cttaatagaa tctataattc
     6661 aaatcttgag tgatgaattg gtttcagact aaaacatggg ttgatttggt gcgacatctg
     6721 tttttaatat ttgtcattgt cactgtgttg gttcttgggc tattttacat ttacctgcca
     6781 gcaaaaacca atcatggtga ggcaattaca gttccggacc taatcggctt aaatatctct
     6841 gacttagaca aatttctggt tgaaagaaat ttaagatacg aaataaatga ttccagttac
     6901 gaccacgtgg ctgaaccatt aactgtttta cagcaattcc ccaaaccagg tgttcttgta
     6961 aaggaagata gaaaaatcta cttaacaatt aatagaaaaa ctcctcccga aattaggtta
     7021 cctgcgtcaa taatcgggta ttctctaaaa aacgcggaag cggttcttac aagttatgaa
     7081 ttactaagag gtaaaattac ctatgttccc tatccttact ctaacctggt attggaaatg
     7141 aaactgcagg gaaaaaatct ctatgaaggt aaccgtgttc caaaaggttc ggctattgat
     7201 ctggttgtcg gcgatggata cggaaaaagg aaatttgcca ttgatgacct gacacggttg
     7261 acactcgatg atgcaaaaat atatctaaat ggttccggat tgaaattagg aatcgtgacc
     7321 ggacaaaaag actcaacgga tacgaattgg attgtatcca aacaaattcc actccctaac
     7381 gatatggtta gcattggtca gttcgttgat atatgggtag cgcctcttga ctcgttaaat
     7441 aatgagcctt tggatgaata gttggagaat gatgaccatg aagagttcta gtcttttttt
     7501 gttaatagta acattcctgg tacaggctca cgcccagatt aaggttgtcc cgatacaggg
     7561 agttactcat tccaaaaaaa ataatgcata cagtacacag aattacagaa ttactgccga
     7621 aaagttggta ttgcctttat gggaagattt tagcacctat gaaggccaac cggatacaac
     7681 attatggatt aatagcgcta acgtttgggt aaatcatggt atgtgcatta actgcccaag
     7741 tattaatgtt gccacttttg atggacttga taagaatggt aacccttaca gtctggaccc
     7801 ctccgatatt ggtctgacag atagcctggt ttcaaaacca atagatctta cccaggtggc
     7861 ttttgcgaaa agaaactctg tctacttaag tttttattac caaaaaggcg gccaaggaga
     7921 accacctgat gcccaggata tgatagaact tcagttcaga actgccggta atagatgggc
     7981 tcgtgcctgg ccggcagatc cggaatctct taattcactc acttcaatat ttaatggagt
     8041 ttctattcag attgttgatt cactttttca tgataatttt caatttagat ttctttctac
     8101 cggaagaaaa acagggtcgt ttgatgtctg gaatattgac tatatttatc tggacaaaaa
     8161 cagaagtcag gatgattatg aaattcttga ccgcgcaatc acatcaatcc caaattcaat
     8221 tttcaaaaat tacaccgcaa ttccgctcag gcaaatttac tttggagatc aatcaatcat
     8281 agattcaact gaagtgcggc tgtttaatta cgaagtcagc gaacccgttc aagggataga
     8341 gtattcggcg attttgaaaa atactaaaac tcaacttgaa attgagaaga taatagataa
     8401 gaaagcacat ataataatgg caaataactt cctggattta aaagccggaa aaccaaccct
     8461 tcaacttttg aaagaccagg tatcaggacc cgacgattca ttgtttctcg aactcattta
     8521 ttatgtagat accggggata cactcctaat cgacgaaata aacacgacaa ctaatgatac
     8581 cacatatatt agttacattg atttaagagt aaatgataca ataaccagaa agtttactct
     8641 gcataattac ctggcctatg atgacggaac tgctgaattc ggggccggaa taaaccaaaa
     8701 tcaaggtcaa attgcttata agtttttcat tcaacagcct gacacgctag ttgctttcaa
     8761 tattaacttc cctaacatca acggaaatgt tgccggtctc ccaattgact tgattgtatg
     8821 gagtcatctc gacggatttg cagattctga attatacaga cagccattta caattcaggc
     8881 aaatggaatc aatgaactta aaacttatga gctgttgaaa cctctagcgg ttgaagacac
     8941 cttctacata ggttttcagc aatcaactaa tgaatttatt ccagtggggc tggataaaaa
     9001 ttccgactcc ggtgagaata tattttataa cgtgaatggt gtttggcaag ccaatacaga
     9061 actaatcgga agtttgatga tgaggcctgt atttggcaaa ggaggtgtta ttactccgat
     9121 aaattctgaa atcccgggaa taaatatcta tcctaatcca accgatggta attttacggt
     9181 taccggaaat cccactgagg ttaatattta tgactttagc gggcgtaaaa tgaaagccac
     9241 cttcatcgca acctcatcag gagttgctgt ttcagcaata ggttataaac agggtattta
     9301 cctggtagaa gtaattactc cctctcaaag gataatccaa aaattgattt tcagataatt
     9361 aggttcctta tctttaaccc atgattaaca aatcagatac ccttgaacaa acaaaagtat
     9421 acatttcagc tgatgacttc gcgaacataa atacccaaaa ggaattaatt aagattctgg
     9481 ataaaaagaa tatcaattac tctaaacctc ttcgcatctt gcggtacgaa agggaattgg
     9541 ttaaacttca aattgagttg gttaagcttc agacctggat tagggacact aaaaaaaggg
     9601 ttgtagtaat ttttgaaggc agagatgcag caggtaaagg aggtaccata agaagattta
     9661 gagaacatct gaatccaagg gctatgaggg ttgttgcgct aacaaatccc aatgaggttg
     9721 aaaaaggaca gtggtatttt cgaagatatg tgaaagaact tcctaacccc ggggagattg
     9781 ttttctttga tcgaagttgg tataataggg cggtggtgga acctgtaaat aaattctgct
     9841 caaaaaaaga ttactataaa tttattcaac aagttcctga attcgaacat atgctgtatg
     9901 aagatggagt caaaataata aaattctggt tctcgatttc caaggaggaa caattgaaaa
     9961 gatttgaatc ccgaaaaaag aatccgttaa agcaatggaa attgagtcgt gttgatgaaa
    10021 gatcccagga gctttgggat aagtacacct actacaagga agaaatgttt tcaaagacgc
    10081 atacaaccta tagtccatgg atcattgtcg aaagtaatag caaaaggaaa gcaaggcttg
    10141 aaagtatgcg ttatgttctg agcaactttg attacccggg taaagagagt gcccgcacaa
    10201 ctttatatcc ggatcccaat acggtaaaac gttttcatcg atcagtactt cagatagatt
    10261 agattggaac tttcaacaat tgatattgaa ctgctgaact ctcaggatgg cctgaaacaa
    10321 cttctcaaat ccaagaagtt aaatatgaga aaaattctga actaccttcg atacgaaaag
    10381 gaattaagag aacttcaaac agaagcaata aaacttcaaa actggatcac caataataat
    10441 aagcgtttgg ttatccttta cgaaggcaga gacagtgccg gaaaaggcgg tgccatcaga
    10501 cgttttgttg agcatttgaa ccccagacgg tacaaagtgg tggcattgcc aaaacccaca
    10561 attaaagaaa aaggccagtg gtactttcaa agatatgcac aacgccttcc agaccctgga
    10621 gaaattgtcc tgttcgatcg tagttggtac aacagggcaa ttgtggaacc tgtaaatgga
    10681 ttctgcaatc aaaaacagta caaaaggcat cttgaacaga ttcctgagtt tgaaaaaatg
    10741 ttaaaggaag atggaaccat cctaatcaag ctttggtttt ctatttccaa acaagagcag
    10801 aagaaaagac ttattgcaat caaaaaaagt ccgctcaaac aatggaaact aagcccggtt
    10861 gatgaaaagg cccaggagct ttgggatgtt tatactcaat acaaagaaga aatgttttcc
    10921 cggacgaata ccaaagaaaa tccatggatt gttgttatgg cagattctaa gatcaatgcc
    10981 agggtggaaa gtatgaagta tatccttgaa caaataccct acgccggcaa atctgaaaac
    11041 cttgaatgga acaggaatgt aattgtccaa atttagattc cttcaacaat aattggcctt
    11101 ggactaaagc tcagcacaaa aattaataat gcaatccacc caagaatctt tctgttaaaa
    11161 cttatcggtt catcatacaa agtgggcgga tgctgaattc ccaaaaacct tccgataata
    11221 aaagcaaaaa taagccaacc ctgataccca attactccgg gaaataagta caccactcca
    11281 aattgagccg tgaaagtgag aacagctata aataatctgt ctctgaattg tttggaaatg
    11341 gtatagtagg ttaaataaag gaagccgaca tagaggatgc tgtacaatac agtttctgca
    11401 aagcccacat tgggatcaaa tggattaatc actcccaggc cggcatagaa tacaaacgca
    11461 ataaaaatgc ccgacgaaat tatcctgtga ttttccctgc cgaaaagtcc atacagaaca
    11521 tgccctccat ctaactggcc aactggaata aggtttagag cagtaaaaaa aagtgccaga
    11581 aagcccgcga ataaccaggg ataatgcatt gcctcataat gattgggaag tcgttcctta
    11641 tcggcaacat tatttttaaa gaattcaaat atcaggttag tccccaggta aactttaggt
    11701 atttgggtag agaattcaat agtgtcaggc aatagattta ataattcgct ttcagggt
//
LOCUS       DASWHX010000028         8334 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_3344192, whole genome shotgun sequence.
ACCESSION   DASWHX010000028 DASWHX010000000
VERSION     DASWHX010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 8334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8334
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3344192"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..413
                     /locus_tag="VGA21_01495"
     CDS             <1..413
                     /locus_tag="VGA21_01495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649208.1"
                     /translation="GDFYRYQMNRMIDRNTHHVDVLEVIVNDCVAEGELFRVTDLGRS
                     VFNDENVDGLKLQLFIEVAFEYVKGDSAWVFLQSLDKNAATFYRELDEQLLARYNPFV
                     EPVFIKSKIGDAIGVFGSAVRSDSILLIYPVSAD"
     gene            complement(879..1463)
                     /gene="purN"
                     /locus_tag="VGA21_01500"
     CDS             complement(879..1463)
                     /gene="purN"
                     /locus_tag="VGA21_01500"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009578695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEX9649209.1"
                     /translation="MKHSIAIFASGSGSNAEKIINHFRKSNSAEIKLIISNKNDALVI
                     ERARKLQVPYVIFTRKSDFSGDVLFKLLLDNNIDLIVLAGFLWLLPANLIEKFPSRII
                     NIHPALLPRYGGKGMYGARVHQAVSESGDTESGITIHYVNEHYDEGDIIFQASCSIRA
                     GENPESIAHKVHQLEYEYYPKVIEEVLNKFQSNL"
     gene            complement(1460..2341)
                     /locus_tag="VGA21_01505"
     CDS             complement(1460..2341)
                     /locus_tag="VGA21_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009578694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranylglycerol-phosphate
                     geranylgeranyltransferase"
                     /protein_id="HEX9649210.1"
                     /translation="MSEPDQPKTFSIPGFFQVTRIQNLSVIILTQYLAAIFLINNYES
                     ANRLQEIDLFILVISTVLIAAAGYLINDYYDVKIDLINKPKKVVVGTVLKRRSVMMAH
                     TVFNLLGISLGLIIDYRIGIINFAAAFILWLYSNQLKRTLFLGNLVVAALAGLTIMVI
                     GIFYHQLNMAIVLYSLFAFTSNLVREIVKDIEDLQGDVKFGSKTLPIVLGVRRTKGVL
                     LLINASFFIFILYLFSGSRFPGPDLVLYFILVPLILLSGMLIRADKKRDFNILSSSIK
                     WFMLIGIATMMLIRYPA"
     gene            complement(2379..3167)
                     /locus_tag="VGA21_01510"
     CDS             complement(2379..3167)
                     /locus_tag="VGA21_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008627672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rossmann-like and DUF2520 domain-containing
                     protein"
                     /protein_id="HEX9649211.1"
                     /translation="MAEGLRCALIGAGNVAWHLGPELENAGYAVHEVYSRNPRNAATL
                     VERLYHAEVKKDLDFSSSSSQLFIICVSDDAIKDVVTEIALPEESILVHTSGAQPLSI
                     LDYAATEHIGVLYPLQTFSKQRKISFKEIPIFIESDDMPVINVLSDIAESIASRAVII
                     DSKSRKALHVAAVFACNFTNHMLTLSKSISESNDLDFGLLQPLIVETITKSLEIGPEF
                     SQTGPAIRKDLQTLDNHLEFLANDEEVQKIYRIITQHIIDYYDI"
     gene            complement(3177..4469)
                     /locus_tag="VGA21_01515"
     CDS             complement(3177..4469)
                     /locus_tag="VGA21_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019987849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX9649212.1"
                     /translation="MNPTRRTFFRKFAGIAGTLSISTTLARAAAEDVSDALLNLKKLS
                     PAEAAMEEEIWNSIRQAYTVSANIINLNNGGVSPQPKVVQDAVDRYYHLSNEAPSYYM
                     WRILDMGREPVREKLAHLADCSPEEVAVDRNATEALDTAIFGMRLEKGDEVILTKQDY
                     PNMINAWKQREMRDGIVLKWLNLELPENNEDKLVNSYTGAFTSKTKVVHITQMINWNG
                     QIIPAKRIVAEARKRNIISIVDGAHTFAHFYFSLSEMAPDYFGTSLHKWLCAPFGTGM
                     MYVRKENIADTWPLFPNHEPLSDNIRKFEGLGTRSFAPEQAIGQALDFHNAIGSKRKE
                     ERLRFLKNYWCKNVMDIDRVKLNISLEPHASCALGNFSIDGIDPGDIANKLFSDYKIH
                     TVGIKWENINGVRVTPHVYTNLYDLDKLVDAIHTISRS"
     gene            complement(4538..7072)
                     /gene="ccsA"
                     /locus_tag="VGA21_01520"
     CDS             complement(4538..7072)
                     /gene="ccsA"
                     /locus_tag="VGA21_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018472166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HEX9649213.1"
                     /translation="MVHTFIGNLGHFFVITSFVTAILAAFSFFKANAKSDLHHKVPWL
                     KNGRTLFYIHFAAIIGVVGSLFFIIYNHYYEYHYAWSHSSLHLPTQYMISCFWEGQEG
                     SFLLWMFWQAAIGVVLIRTNKFWEAPVMTVFSLVQTFLASMILGVVIFNLKIGSTPFI
                     LLRDFMINAPVFQINPDFIPEDGTGLNALLQNYWMVIHPPTLFLGFATTLVPFAFCIS
                     GIWNKKYKEWIRPALPWTIFSAAVLGLGILMGGYWAYETLNFGGYWNWDPVENAVYIP
                     WLFLIASIHTMITFKNSATALKSSIVLVIITFILILYSTFLTRSGILGDASVHSFTDL
                     GLSGQLLIYLLFFTVGAVILSAFHWKNIPTSTREASTYSREFWIFIGATTLGLMSFQV
                     LIPTSIPVFNKILQLIGIPSQAALPADQIQFFTTWQKWFAIAVLLLSGTGQFFWWKKM
                     EKTKLFNELIGPISMSLIISAVIILISGISNLSYILLLTASIYSIIANGKIIYQLSKN
                     NISLSGGGVAHIGIAMMLIGILTSSGYSRVVTTNATGTVIHPDFTSEFNLENVLLVLN
                     ETKNIGGYTLKYKGQRMEAKGIPGYVKKNDLGPTDDPFLAFVKKEISANGKIYASKGD
                     TVEIYPENTYYEVEYVSQSGKRFTLYPRAQINEDMGGILASPDIHRRVISDLYAHVSS
                     IPDPTSPVQWDEMKEIRVKAHEKFFVNDYVSEIESMERITEVEGVALGPADVAVKARI
                     KILGDEDEYELEPIFLIKDRLVGRIPAVEHDLGIKITFLEVHPDDNEFTLGLNTTQKD
                     YVILKAMEKPFINILWSGTFILMFGFGIAIYRRYKEFTKIRDKEKE"
     gene            complement(7080..7493)
                     /locus_tag="VGA21_01525"
     CDS             complement(7080..7493)
                     /locus_tag="VGA21_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014771380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017003 - protein-heme linkage [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HEX9649214.1"
                     /translation="MKRSHIFALIVIAVAVMIIISTAGDASTYVTFSQAKEMSINGNS
                     SKIHVVGELTKNEIGEVVGLKSSDNLLSFTFTMKDEENNIQNVFYSEPMPHDFLRSEK
                     IVVIGAYRNEMFVADKILLKCPSKYQETEIKTAGK"
     gene            complement(7532..7756)
                     /locus_tag="VGA21_01530"
     CDS             complement(7532..7756)
                     /locus_tag="VGA21_01530"
                     /inference="COORDINATES: protein motif:HMM:NF040113.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649215.1"
                     /translation="MKKFTSLLLGLIWLSLSSFQPDEIEMADGLRSGGLIYIVVMVVL
                     IMVLGLVFYLFTLDMKISKLEKESSEPKKE"
     gene            complement(7753..>8334)
                     /gene="ccsA"
                     /locus_tag="VGA21_01535"
     CDS             complement(7753..>8334)
                     /gene="ccsA"
                     /locus_tag="VGA21_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009195276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HEX9649216.1"
                     /translation="LHETIRVLYFHVPMWFGMVILLTVSMVYGIRYLRNGDEQLDDMS
                     LSFAKTGIVFGVLGLITGMVWANYTWGTPWHGDPKQTAAAIAMLIYFAYLVLRNSIED
                     QQRKARVSAVYNVFAYAALIPLLFILPRLTSSMHPGSGGNPGFNTYDLDSNMRLVFYP
                     AVLGWTFLGIWISLLTFRFRRLYSERMKKIYES"
BASE COUNT         2568 a         1688 c         1689 g         2389 t
ORIGIN      
        1 ccggtgattt ttaccggtac cagatgaaca ggatgataga caggaacact catcatgtgg
       61 atgtgctgga agtaattgtg aacgattgtg ttgctgaagg cgaattattc cgggtaacag
      121 atttgggaag aagtgtgttt aacgatgaga atgtggatgg tttgaaatta cagttattta
      181 ttgaagttgc gtttgaatac gttaagggag actcagcctg ggttttcctg cagtcattag
      241 acaaaaacgc cgcaacattt taccgtgaat tggacgaaca gttactggct cgatacaacc
      301 cgttcgtgga gccagtattt atcaaatcga aaatcggaga tgcgattgga gtttttggct
      361 cggccgtgcg ctcggattcc attctgctta tttatccggt gagcgcggat tagaaaataa
      421 ccggaaagta cagaaattca tccacaacta cttagcagag ggaatagttt tgaattaaaa
      481 gcattgttta ttttgggtga aagtgaagaa ctttcactct gaattttctt cgctctaata
      541 ccgggggcca ggtcttgatt tgtaattgta tcctgtatag ctgccaggag atgccgattc
      601 gttgaaattc ttctactgaa tcccccaaac tttaccatga tttccttcgg tttcctgagt
      661 cggtgagtga taatataccg atgcgggtca gtttcgcttc ccctaatcag atattcttct
      721 ataaaagaac ccgtcaatta gcaaaaactt tgaattcatt tttcaaatat gcaggtctgc
      781 ttcaatttta aaaagtgaat gtcgaatatt gaacaagaaa taaaggaaat tcaatcgaat
      841 tcacacagaa attggtactt gttattgcaa tctctgattt agaggtttga ttgaaatttg
      901 tttaacacct cttcaattac cttcggataa tattcgtatt ccagttgatg aaccttatgg
      961 gctatcgatt ccggattttc ccccgctcgt attgagcagg aagcctgaaa aatgatatct
     1021 ccttcatcat aatgttcatt cacatagtga atggttatac cggattccgt atctcctgat
     1081 tctgagacag cctgatgaac cctggctcca tacattcctt ttccaccata cctgggtaat
     1141 aatgcgggat gaatattaat aatgcgactg ggaaattttt caataagatt tgcggggagg
     1201 agccagagaa atccagccag aactataaga tcaatgttgt tatcaagtag aagtttgaat
     1261 aagacatcac ctgaaaaatc agatttcctg gtgaaaataa catagggaac ctgaagtttc
     1321 ctggcgcgtt caataacaag tgcgtcattc ttattagaaa ttatcaattt gatctctgcc
     1381 gaattggatt ttctgaaatg gttgataatt ttttcagcat tggaaccgct gccggaggca
     1441 aaaattgcaa tagaatgttt catgccgggt accggattaa catcatggta gcaatgccta
     1501 tgagcatgaa ccattttatt gaggagctga gaatattgaa atctcttttc ttatctgcac
     1561 gaatcagcat ccccgacagg agaattaacg gtacaaggat aaagtaaaga acaaggtcag
     1621 gtccgggaaa tctggatccg gagaacagat ataaaatgaa tataaaaaac gatgcattaa
     1681 taagcagaag aactcccttt gtccttcgaa cgcctaaaac aataggaaga gttttgcttc
     1741 caaactttac atcaccctga aggtcctcaa tatctttaac aatttctctt accaggttgg
     1801 aggtaaacgc aaataaggag taaagaacaa tagccatatt cagctgatgg taaaagatcc
     1861 caattaccat aatggtaagt cctgcaagtg ctgcaaccac caggttgcca agaaatagtg
     1921 ttcttttcaa ttgatttgaa taaagccata aaataaatgc agcggcaaaa tttataattc
     1981 caattctgta atcaataatc aatccgaggg atatccccag gagattgaaa actgtgtgtg
     2041 ccatcattac agatctccgc ttaagaacgg tacctactac tacttttttg ggtttattga
     2101 tcaaatcaat tttcacgtca taataatcat tgatcaggta tccggcagca gcaatcagaa
     2161 cagtagatat aaccagaata aacaaatcaa tttcctgcaa tcgattggcg gattcataat
     2221 tatttatcag aaaaatagcg gcgaggtatt gggtcaaaat aattactgaa agattttgaa
     2281 ttcgggtaac ctggaaaaac cccgggatag aaaacgtttt aggctgatcg ggttcgctca
     2341 ttcggtatta cttgaattca attaaatgga attctctttc aaatatcata ataatcaata
     2401 atgtgttggg taattattct atagattttc tgaacttctt catcattcgc aagaaactca
     2461 aggtggttat ccagagtttg aaggtctttt ctaatggcgg ggccggtctg agaaaattcg
     2521 ggcccaatct ccaaactctt tgttattgtt tcaacaataa gaggttgaag aagcccaaaa
     2581 tccaggtcgt tgctttccga gatggatttt gacaatgtta gcatgtgatt ggtaaagtta
     2641 caggcaaaca cagcagctac atgcagggct ttcctcgatt tggaatcaat gattactgcc
     2701 cgtgaagcaa tggactccgc tatatctgaa agaacattga ttacaggcat gtcatcgctt
     2761 tcaataaaaa tgggaatttc tttaaacgaa atttttcttt gtttactaaa agtttgcaat
     2821 ggatacaaaa ctccaatatg ttcagtggct gcataatcaa gaatgcttaa tggctgagca
     2881 ccggaagtgt gtacaaggat cgattcttca ggcaatgcaa tttcggtgac cacgtccttg
     2941 atggcatcat ctgacacaca aatgataaat aattgggaac tactggagga aaaatccaaa
     3001 tcctttttga cctcagcatg atacaatctt tctacaagcg tcgctgcatt cctggggttc
     3061 ctgctgtata cttcatggac tgcatagccg gcattttcaa gctcaggacc aagatgccag
     3121 gcaacattac ccgcaccaat taaagcacat cgcaatcctt cggccattgg gaaaaattaa
     3181 ctcctggaaa tagtgtggat agcatcaacc agtttatcta aatcataaag attagtataa
     3241 acatgcggag tgactctaac gccattaata ttttcccact tgatgccgac ggtatggatc
     3301 ttataatccg aaaatagttt atttgctatg tctccgggat caattccatc aatactaaaa
     3361 ttacccaagg cgcaggatgc atggggttcc agggaaatgt taagctttac ccggtcaatg
     3421 tccattacat tcttgcacca gtaatttttt aaaaagcgaa gccgttcttc tttacgttta
     3481 cttccaatgg cattatgaaa atcaagagcc tggccaattg cctgttcggg tgcgaatgag
     3541 cgcgttccca gaccttcaaa ttttcgtatg ttatcactta gaggttcatg gttcgggaaa
     3601 agcggccatg tatcggcaat attttctttt cgcacgtaca tcattccggt accgaagggt
     3661 gcgcacaacc acttgtgcag gctggtccca aaataatcgg gtgccatttc acttaaggag
     3721 aaataaaagt gcgcgaatgt atgagctcca tcgacaatac taatgatgtt acgtttcctg
     3781 gcttcggcga caattctttt tgccgggata atttgtccat tccagttaat catctgggta
     3841 atatgaacca ctttggtttt agaagtgaaa gcaccggtgt aagaattaac caatttatcc
     3901 tcattatttt cgggaagttc aagattcagc catttcaaga caatcccatc ccgcatttct
     3961 ctttgcttcc aggcattaat catatttgga tagtcttgct tagtgagaat tacctcatca
     4021 cccttttcca ggcgcatccc aaaaatggca gtatccagtg cttctgttgc atttctgtcc
     4081 actgcaacct cttcgggaga acaatctgcc agatgtgcca acttttcacg aaccggttca
     4141 cggcccatgt ccagaattct ccacatataa taagagggag cttcattgct caaatgataa
     4201 tagcgatcaa cagcatcctg aacaactttc ggctgcggac ttacaccccc gttgttcaag
     4261 ttgatgatat tggcggaaac tgtgtaagcc tggcggatgg agttccaaat ttcctcctcc
     4321 atagcagctt ctgcaggtga taatttcttc agattcagca aagcatctga gacatcctcc
     4381 gccgccgccc tggccagtgt tgtacttatg ctcagtgtac cggcgatacc ggcaaatttt
     4441 ctaaaaaacg tacgtcgcgt gggattcata atccttgaat tttaataata ctgttaagtt
     4501 atggaaatta tgattagcga taaacggcaa aaagtattta ttctttttcc ttatcgcgga
     4561 ttttggtgaa ctccttgtat ctacgataga tggcaatacc aaatccaaac atcaaaatga
     4621 atgtgccgga ccataaaata ttgatgaaag gtttttccat cgcttttagt atcacgtaat
     4681 ctttttgagt ggtatttaat ccaagggtga actcattatc atcaggatga acttccagga
     4741 aggtaatttt aatacccagg tcatgttcta ccgcaggtat cctgcccaca agtcgatctt
     4801 ttatcaggaa aatgggttca agttcgtatt catcctcatc acctaagatt ttgatgcgcg
     4861 ctttaaccgc tacatcggca ggtcccagtg caacgccttc cacctcggtt atcctttcca
     4921 tgctttcaat ctccgagaca tagtcattga caaagaattt ttcatgagcc ttcacgcgaa
     4981 tctctttcat ttcatcccat tgaacaggcg aagttggatc gggaattgaa gaaacatgcg
     5041 catatagatc ggatataacc cgacggtgta tatcaggaga agccaaaata cctcccatgt
     5101 cttcgttaat ttgcgcccgt gggtataagg taaatctctt gccggattgg gaaacatatt
     5161 caacttcata gtaggtattt tcgggataaa tttcgaccgt atcacctttt gaagcataga
     5221 tttttccatt tgcggaaatt tcctttttca caaaagcaag aaaaggatca tcggtgggtc
     5281 ctaaatcatt tttcttaaca tacccgggaa tgccttttgc ttccattcgc tgacccttat
     5341 actttaaggt atacccgccg atatttttgg tttcattcag cacaagcaac acattttcaa
     5401 gattaaattc ggaggtgaaa tcaggatgaa tcacggttcc ggttgcattg gtggtaacta
     5461 ccctcgagta gccggacgaa gtgaggatgc cgatcaacat catagcgata ccaatatgag
     5521 ccaccccacc ccctgaaagt gaaatattat tttttgatag ctgataaata atctttccgt
     5581 tggctatgat tgaataaatc gatgccgtta gcaaaagaat ataggataaa ttgctgatgc
     5641 cggaaatcaa aatgatgact gcggaaatta ttaatgacat ggatatcggg ccaatcaatt
     5701 cattaaataa cttcgttttt tccatctttt tccaccaaaa aaactgaccg gttcccgaca
     5761 agaggagaac tgcaatggca aaccattttt gccatgtggt gaaaaattga atctgatcag
     5821 caggtaatgc cgcttgtgat ggaatcccta tcaattgaag tatcttgttg aaaacaggta
     5881 tggaggttgg tattaatacc tggaaggaca ttaaacctaa agtggtggca ccaatgaaga
     5941 tccaaaactc cctggagtag gtagaggctt ctctggtgga ggttggaata tttttccagt
     6001 gaaaggcaga taaaatgaca gcgccgaccg tgaaaaataa aaggtaaatt aatagctgac
     6061 ctgataatcc taaatctgta aagctgtgta cagaagcatc acctaaaatt ccgctgcggg
     6121 ttaggaacgt ggaataaagg atgaggataa aagtgataat taccaacacg atggaagact
     6181 ttagcgctgt ggccgaattc ttaaaggtta tcatagtatg gatactggca atcaggaata
     6241 gccacgggat gtatacggca ttttccaccg gatcccaatt ccaatatcct ccgaagttca
     6301 aagtttcata ggcccagtaa cctcccatta ggattcccag acccaacacc gcagcggaga
     6361 agattgtcca gggcaaggcg ggccgaatcc attctttata ttttttattc cagattcccg
     6421 aaatgcaaaa tgcaaaaggg actaaagttg tagcaaaccc caggaaaaga gtaggaggat
     6481 gaattaccat ccaatagttt tggagcaaag cattgagtcc cgtaccatct tcgggaataa
     6541 aatcagggtt tatctgaaaa acaggagcat taatcatgaa atctcgtagc aaaataaatg
     6601 gagtagagcc aatttttaaa ttgaagataa caacccccaa aatcatggaa gcaaggaaag
     6661 tttgaaccaa tgaaaaaacc gtcatcaccg gagcctccca aaacttattg gttcggataa
     6721 gcacaacacc aatggctgcc tgccaaaaca tccagagaag aaagcttcct tcctgccctt
     6781 cccagaaaca agaaatcata tattgagttg gaagatgcag ggagctgtga ctccaggcat
     6841 aatggtattc ataataatga ttgtaaataa taaagaaaag tgaaccgacc actccaatga
     6901 tcgcggcaaa gtgaatataa aaaagggttc ttccattttt cagccatggc actttgtggt
     6961 gaagatccga ttttgcattg gctttgaaaa aagaaaatgc ggcaagaatt gccgttacaa
     7021 atgaagttat gacaaagaaa tgaccaagat taccaataaa agtgtgaacc attggtttat
     7081 tactttcccg cggttttaat ttctgtttcc tgatacttgg aaggacactt tagcagtatc
     7141 ttgtcagcaa caaacatttc gttccgatac gcgccgatga caacaatttt ttctgagcgt
     7201 agaaaatcat gcggcatggg ttcactataa aaaacattct gaatattatt ctcttcatcc
     7261 ttcatggtga aggtaaatga aagtagatta tcactgcttt ttaatccaac cacttcgcca
     7321 atttcattct tggtcaattc tccaaccaca tgtatcttgc tactgttacc atttatggac
     7381 atctcttttg cctggctgaa cgttacgtag gtacttgcat cgccggcggt ggaaattatg
     7441 atcataacgg ctactgcaat aacaatcaga gcgaaaatat gtgatctctt catacccggt
     7501 aacttcaaac tcttttcaaa gttacttaat attattcttt tttaggctca gaagattctt
     7561 tttcaagctt tgagatcttc atgtccagcg taaagagata gaatacgagt ccgagaacca
     7621 taataagcac caccatcact acaatgtata ttaaaccacc ggagcgcagt ccatctgcca
     7681 tttcaatttc atcaggctgg aagctgctga gggaaagcca tattaagccg agaaggagac
     7741 ttgtaaattt tttcatgatt cataaatttt tttcattctt tctgaataaa gtcttctgaa
     7801 ccggaaagtt aataaggaga tccagatacc aagaaaagtc cagccaagta cggcagggta
     7861 gaagacaagt cgcatgttgc tgtcaagatc ataggtatta aagccaggat ttcctccgct
     7921 gccagggtgc atggagcttg ttaatctggg taaaataaat agcaaaggta taagggccgc
     7981 gtaagcgaag acattataaa cagcacttac acgggctttt ctttgttgat cttcaataga
     8041 gttacgcaaa accaggtagg cgaaataaat caacattgca attgcggccg cggtttgttt
     8101 agggtctccg tgccagggag ttccccaggt gtaatttgcc caaaccatgc cagtaataag
     8161 acctaaaact ccgaatacaa ttccggtttt agcaaatgaa agagacatat catccaattg
     8221 ctcatcgcca ttccggagat acctaatgcc gtaaaccatt gagacagtaa gaagaatcac
     8281 cattccaaac cacatgggaa catgaaagta caaaacccgg atggtttcat gcag
//
LOCUS       DASWHX010000029        15166 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_391251, whole genome shotgun sequence.
ACCESSION   DASWHX010000029 DASWHX010000000
VERSION     DASWHX010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 15166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15166
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_391251"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1136)
                     /gene="lpdA"
                     /locus_tag="VGA21_01540"
     CDS             complement(<1..1136)
                     /gene="lpdA"
                     /locus_tag="VGA21_01540"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008202812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEX9649217.1"
                     /translation="MSKFDLIVIGGGPGGYVAAIRASQLGMNVAVVERAELGGICLNW
                     GCIPTKALLKSANVFEYIKHAEDYGIKVKESKADLVSMVKRSRDVADGMSKGVQFLFK
                     KNKIQHIAGFGRLKGSGKVEVEKDGKKTEYTANNIILATGGRSKELPNLMIDGKKIIG
                     YREAMTLPSLPKKMVVVGSGAIGSEFAYFYNTLGTEVTLVEFLPNLVPLEDEEVSKQL
                     ERSFKKAGIKTMTSSEVTSVDTKGKGCKVTIKTPKGEEKVDCDIVLSAVGISTNLESM
                     GLEEVGVAIDKGKVIVDQYYKTNINSIYAIGDIVKGQALAHVASAEGITCVEKIAGMN
                     PEPLDYNNMPGCTYCSPEIASVGYTEKAAKEAGYEIKVGKFPFSA"
     gene            1303..2049
                     /gene="fabG"
                     /locus_tag="VGA21_01545"
     CDS             1303..2049
                     /gene="fabG"
                     /locus_tag="VGA21_01545"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015268304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEX9649218.1"
                     /translation="MNLLEGKIALITGASKGIGRAIALTFAEHGANVAFTYLSSVEKG
                     EALEKELSARGVKAMGFRSDASDFKTAEELIAGVVREFGSLNVLVNNAGITRDNLLLR
                     MSEEQWDEVLRVNLKSVFNTVKAATKTFMKQKGGSIINITSVVGIKGNAGQANYAASK
                     AGIIGFTKSIAIELGSRNIRSNAIAPGFIETEMTDVLDQNTVQGWRDSIPLRRGGKPD
                     DVANAAVFLASDLSDYISGQVLQVDGGMLT"
     gene            2325..4781
                     /locus_tag="VGA21_01550"
     CDS             2325..4781
                     /locus_tag="VGA21_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020531534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9649219.1"
                     /translation="MFKNYLKIGLRALVKQKIYSFINILGLSVGIASCALILMHVEDE
                     FSYDQFHLKKNQLYKIGLERKYPNHTTYFDLIPHSFMEVMVEDYPEVVAGARIADFTG
                     GGQFTLMMRYIDEKNNEKAFEESRFLAADSNIFDMFTIEVLNGDKEKALRGANKVVIT
                     EETAKKYFGDADPIGKTLSAPFAEFQVTAVCENIPENSHFDFDFLASLSSGGNFFQQT
                     NFTGFSTHTYLELEPDANPKALEAKFPAMVDKYAGPQIEQNLSIAYADYVAAGNGYRY
                     IMRQLTDVHLDPTNYESKMKPGGNLTYVYIFISIAIMIMIIACINFMNLATARSAERV
                     KEVGIRKTMGSHRRQLVSQFLTESIVISFVSMLFAFLIIYLALPYFNTLAQKNLALNI
                     TGSMIVPMMFIFALIVGLLAGSYPAFVISGYSPVSVLKGKLHSSGRGSWLRDGLVIFQ
                     FFVSIILIVGTLGVKKQMDFMRNKSLGFDKDHVIYVTGTQVLGQNFESFVNEIRRMPG
                     VESAAGGQSVPGLFTFGAQFTKEGATEPLTTKSFNMSDDFAETLGFEIVDGRDFSKDF
                     DDSLSLILNEVAVRTLGLEDPVGSRLSTNAGGGNGGNRIFTVVGVVKDYHFQSLRDEI
                     TPLAIFCTESNGPGPNFLVAIRTESRNLGDLVLSVENLWNRLAVNNDPFKYKFLDESL
                     EAQYRDEKRSGELFGIFAGLAIIIACVGLFGLAAYTAGLRTKEIGVRKVMGASVAQIV
                     AMLSIEFTKLILIAFVLAVPMAWWGMDNWLNGFYYRTTLGISTFFIAGGSALLISWLT
                     VSYQSIKAAIVNPVNSLRSE"
     gene            complement(4786..5289)
                     /locus_tag="VGA21_01555"
     CDS             complement(4786..5289)
                     /locus_tag="VGA21_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013927074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX9649220.1"
                     /translation="MRKVILNVAVSLDFMIAGPNGEYDWCFTDQDYGMSDFMKRIDSL
                     FMGSKTYELLLKVDPNSFPGFKRYVFSNSLKEIADAQLIRGNWKATVDTILQTEGKDI
                     WLFGGADLTHHFLQNNLIDEMSLAVHPIILGEGKPLFHSLRERKKFILKETKVFDSGL
                     VQLNYTR"
     gene            complement(5314..6336)
                     /locus_tag="VGA21_01560"
     CDS             complement(5314..6336)
                     /locus_tag="VGA21_01560"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HEX9649221.1"
                     /translation="MKRRQAIKKSIMITGSGFLGSSLILKNLSAGSTEKLTSKSTEKL
                     TFKNIEAPFKISLAQWSLHRKIYNNGNSQPNLREQLLNNPKSVYLGDLDPLDFPVVAR
                     REFGLDAVEYVNVFYFSRARDTNYLTDLKRRADGEGVKSLLIMCDLEGNLGDPDAAKR
                     KESVENHFRWIDAAKFLGCHSIRVNARSSGSFEEQQKLAAEGLRSLCEFAEKSDINVI
                     VENHGGLSSNGKWLSEVIQMIDHPLAGTLPDFGNFNLGNNEWYDRYKGVEELMPFARG
                     VSAKSYDFDDNGNCIETNYRKMLTIVADSGYDGYIGIEYEGGKLSEEDGIKATKKLLE
                     TIFAEM"
     gene            6427..7212
                     /locus_tag="VGA21_01565"
     CDS             6427..7212
                     /locus_tag="VGA21_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009581467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEX9649222.1"
                     /translation="MANTLKIVSWNVNGIRAIVKKDFIKSINRMNPDILCLQETKAPY
                     EEVKTVSTLLNQFPEVHINSSKARKGYSGTAIFLKNSSAEVKADMGIQKHDQEGRVLT
                     ASFPGFFLVTVYTPNSGRGLERLKYRTTWDTDFMKFIKNLEKEKPVIVCGDLNVAHQA
                     IDLANPKSNYNKTAGYTQDEIDGFSRLLESGFVDIFRKRNPELLGVYTYWNYMFNARQ
                     NNKGWRIDYFLISESLVQAVVEAEVYPEFHGSDHCPVGLVINT"
     gene            complement(7209..8819)
                     /locus_tag="VGA21_01570"
     CDS             complement(7209..8819)
                     /locus_tag="VGA21_01570"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEX9649223.1"
                     /translation="MNKITRGITLSLILILVAYLFYPLSSKKFAINHDQDQLDAKNKF
                     LTSIQTGNDSTITPNIIIILADDLGKTDISKYGSKLINTPFIDSIGINGITFSEAYTT
                     ASVCTPSRAGLLTGRYQQRFGMEFQIHETYLTNLMQYYGMKFFVDSDPWYPIRMNSVP
                     NQEDINKQGLPPSEITFGELAKARGYETALIGKWHLGYADELSPRNFGFDYFYGFKSS
                     HHLYAREDNPDIISQRIEEDWTDQYIWGCLGNADCNLYRNEELVEQKGYLTDVLAEEA
                     IKFIDQNQNNPFLLYIPFNAPHTPLQAPGKYIEQFSHIEDPVKRVYLAMIKSLDDAVG
                     KITTTVDKLGLAPNTLIIFLSDNGGATYTKTTDNAPLRGGKITQFEGGVNIPMMMQWK
                     GKIEKGSKFIFPATTLDIFSTIAILTGSGLPKERTIDGKDLMPYITRQVTSPPHESIF
                     WQGGYNKAIRYEQWKLIYNDLTGQTLLFNMAADKFEERNFAEVQPDLVNRLKVLHADW
                     ASKLAAPLWPGMIEFRHREGEEWFGFIN"
     gene            complement(8882..9637)
                     /locus_tag="VGA21_01575"
     CDS             complement(8882..9637)
                     /locus_tag="VGA21_01575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane or secreted protein"
                     /protein_id="HEX9649224.1"
                     /translation="MKTIIFLLLVFTSPSFIKLSEIEKRENLNLQGSWELMSQNGNVA
                     SERRVRIITNGYFSEASFDTMDKDFKGASAGTYTIVGNRLEDVFLSNTWNSDLVNTAE
                     VYEVELAGNTLRKVGRKEGKRWMETWKKLEATEKYPLSGAWRISGRMRDGNFVEMPDR
                     PRKTLKILSENRFQWIALNSETKEFFGTGGGTYEVKDAKYTEHIEFFSRDSTRVGMSL
                     TFDIELTGDGWHHSGKGSTGNPVDEIWIQVDKN"
     gene            9849..10664
                     /gene="speB"
                     /locus_tag="VGA21_01580"
     CDS             9849..10664
                     /gene="speB"
                     /locus_tag="VGA21_01580"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002696183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HEX9649225.1"
                     /translation="MLNQQKTKNTTIALKGIPFDANSSYLPGAAKGPGAIRKALLSPS
                     ANLSTESIIDLGSSCQIIDAGDIQVRDFFPDIVQATREILSSNLSPVILGGDHSITYP
                     VIKAFSEKFPDLTILHLDAHGDLYNDFEGNKFSHASPFARIMEEGLATRLIQVGIRTL
                     TPHQKIQADRFGVEIIEMKHFQIRSIPELASPLYLSLDMDVLDPAFAPGISHYEPGGM
                     STRQVISILQSIKPTLVGADIVELNPDRDFNEMTAMTAAKFLKEIIDNMLRKP"
     gene            10661..11182
                     /locus_tag="VGA21_01585"
     CDS             10661..11182
                     /locus_tag="VGA21_01585"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX9649226.1"
                     /translation="MNFDLNKSLEILKRTPDVLDAQLKDLPAFWSEGKEAVGRWSAMD
                     VVAHLIHGEIEDWIPRAKIILFEKNKQFPPFNPSGFGKILQNKTMDKLLNDFRKLRES
                     NIRELKKLNLSENDFQKTGIHPEFGEVTLHQHLSTWTVHDLNHLYQISRAVGKQYSED
                     IGPWKKYLRLVRD"
     gene            11184..11963
                     /locus_tag="VGA21_01590"
     CDS             11184..11963
                     /locus_tag="VGA21_01590"
                     /inference="COORDINATES: protein motif:HMM:NF017551.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NRDE family protein"
                     /protein_id="HEX9649227.1"
                     /translation="MCLVVFSYKKHLNYKLIIAANRDEFYHRPTQPADFWHDSPNLLG
                     GRDLEANGTWMGVTTEGKVSLLTNYRDLRNLKQNTPSRGHLVSDFLKQEELPKTYLEK
                     LHNISDTYNGFNLVVGNQDDLWYYSNENKRITMLGSGLYGLSNALIDTPWPKLIRAKN
                     KIGDAFNDDFINADKVLDALFDDLRASDDQLPDTGIGFEKEKMLSSIFIKSPDYGTRC
                     STLVTVDQNDNLNFHERTYDLKTFAYSTRLFEFRLNSRVRG"
     gene            11956..12825
                     /locus_tag="VGA21_01595"
     CDS             11956..12825
                     /locus_tag="VGA21_01595"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9649228.1"
                     /translation="MAEKKINVPFILRFIPWIFPKLERIVPLLAYKFAVKLFFTPIRY
                     KIPSTEKEFTKTGEPFLIEVNRKKIRGFSWGEGNTIVGVHGWSGRGAMLRKFVKPLTV
                     SGFRVVLFDGPAHGSSEGFSTDPLEFEGVILEIESKFNPVVGYITHSFGGTAALFCFS
                     RGLKPKPIVLIAVPAIGEDVVQTFTARINGTRKVIKKLEEFTQQRYGKSFYEISAINT
                     ARTIGFTPALVIHDKNDDDVPVEHGEKLMDALPNGTLLRTIGLGHNRILRDPSVVNHV
                     VEFFAHHCSLITH"
     gene            12871..13953
                     /gene="gcvT"
                     /locus_tag="VGA21_01600"
     CDS             12871..13953
                     /gene="gcvT"
                     /locus_tag="VGA21_01600"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018477146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HEX9649229.1"
                     /translation="MEIKKITLNDVHESLGAKMVPFAGFNMPVRYSSDIEEHMQVRNG
                     VGIFDVSHMGEFFLRGPGALDLIQKISSNDATKLFPGRAQYSCLPNKIGGIVDDLIIY
                     MLKENEYMLVVNASNIEKDWDWISSCNDSGVDMENASDKISLFAIQGPKAIEALQPLS
                     SVDLSAIKFYHFEIGDFAGIQDVIISGTGYTGSGGFEIYVKNEDALHTWNKVMETAAP
                     FGIKPIGLGARDTLRLEKGYCLYGNDIDDTTSPLEAGLGWVTRFTKEFINSGNLKEQK
                     EKGVEKKLAGFQMTDRGIPRHGYLIKSEDGNTIGHVTSGTQSPMLAVGIGMGYVKDDY
                     SKPGSIIFIEIRNKLLKAEVKSLPFV"
     gene            13954..14673
                     /locus_tag="VGA21_01605"
     CDS             13954..14673
                     /locus_tag="VGA21_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015813991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HEX9649230.1"
                     /translation="MPSLEICFSPDLLHLYTLENKKVVVADILRATSCMVTGLACGVK
                     SFTPFADLEECRQMRQKGYLIAAERDGQTIDGFDLGNSPFSYMSDEVAGKKVAFTTTN
                     GTQAIQKSGKGEILIGSFLNLQAVTDFLNASGEDVVVVCAGWKGRFNLEDTLFAGALS
                     MNLVNYSINDDAALAAKYLYSISKNNLFQFLSNSSHFSRLKKLNIQKDIEFCLSLNQY
                     SNLPKVINGIVTNNYEGNNPL"
BASE COUNT         4372 a         3092 c         3178 g         4524 t
ORIGIN      
        1 gcggaaaatg ggaatttgcc aactttgatt tcatagccgg cttcctttgc ggccttttcc
       61 gtataaccca cagatgcaat ctcggggctg caatatgtac aaccaggcat gttattatag
      121 tctaaaggtt ccgggttcat tccggcaatc ttttcaacgc aggtgattcc ttccgctgag
      181 gcgacgtgtg ccagagcctg accttttaca atgtcgccga tagcataaat actgttaatg
      241 ttcgttttat aatattgatc aacaatcact tttcccttgt cgattgccac tcccacttct
      301 tccaatccca tactttccag attcgtactt atacccacag cagacagcac gatatcacaa
      361 tccactttct cctctccttt tggtgtttta atggttacct tgcagccctt accttttgta
      421 tccactgaag taacctccga actagtcatg gttttaattc cggctttttt aaaggagcgt
      481 tccagttgct tggaaacttc ttcatcttcc aggggaacca ggttgggaag gaattcaacc
      541 agtgttactt cagttccaag tgtattatag aagtaagcga attcagaacc gattgcgccg
      601 gaacctacaa caaccatctt ttttggaagg gaaggaagtg tcatggcctc gcgatagccg
      661 ataattttct ttccgtcaat cataagattt ggcaattcct tggatcttcc tcccgtcgca
      721 agaattatgt tatttgccgt atattcggtt ttcttgccat ccttttcaac ctctactttt
      781 ccactgccct tcagtctacc aaatcctgca atatgctgaa ttttgttctt cttaaacaaa
      841 aattgaacac ccttactcat tccatctgca acatcgcggc tacgtttcac catgctgaca
      901 agatcggctt tagattcctt aacttttatt ccataatctt ccgcgtgctt tatgtactca
      961 aaaacatttg cacttttaag taaggccttg gtaggtatac aaccccagtt aagacaaatt
     1021 ccacccaatt ccgctctttc cacaaccgct acattcattc ctagctgaga ggctctaatg
     1081 gcggcgacat atcctcccgg ccctcctcca attacgatca aatcaaattt tgacatggat
     1141 ggttacttca aattgagggt caaatttaag gtaaactcgg atagaaaaaa attcagtttc
     1201 ggaagctaaa tgaaggattt tagggtcaat atgaagtcat ccgttggaat tggctatgcc
     1261 ttttccttat tttagtcgcc aaaaaagcat tacaatctta taatgaattt actagaagga
     1321 aaaattgctc taattacagg agcatcaaaa ggaattggaa gagctattgc tctaactttt
     1381 gctgagcatg gcgcaaatgt tgcctttact tacttgtcca gtgtagaaaa aggggaagca
     1441 ctggaaaagg agctatctgc aagaggggtg aaagcaatgg ggtttcgatc ggatgcttct
     1501 gatttcaaaa cagccgaaga attaattgcc ggagtggtca gggaatttgg aagcctgaat
     1561 gtgctggtaa ataatgccgg tatcactaga gacaatttgc tgttgcgaat gagtgaagag
     1621 caatgggatg aagttttgag ggtaaattta aagtcggtgt ttaatacggt gaaagctgcc
     1681 acaaagacgt tcatgaagca aaaaggcggg tcaatcataa acataacttc agttgttgga
     1741 ataaaaggga atgcaggaca ggcaaattat gctgcatcca aagccggaat tatcggattt
     1801 accaagtcca ttgctattga actgggctcc agaaatataa gatccaatgc tattgcaccc
     1861 ggatttatag aaactgaaat gactgacgtt ttggatcaga acaccgtaca aggatggcgg
     1921 gattcaattc cattaagaag aggcggcaag cctgatgatg tcgcaaacgc cgccgtattt
     1981 cttgcatcag atttatctga ttacatttcc gggcaagtgc tgcaggttga cggcgggatg
     2041 cttacctaga tttattcgta cctgagactt tgtgccggat tacgccgacg ctgccttaaa
     2101 agtctgaaag ctaaccagca taattgcaaa aatcaataca actaatcctg acagaagaaa
     2161 aactccaagg gcaattactt agaattagct tatagcttaa aatgccttta tttaagactg
     2221 cccttattga taagtggtta caagaaatct tcattttaat gtccttttgc aacaaattat
     2281 gtgaattgtc agtatactag attaattaat tttcccatct cgctatgttc aaaaactacc
     2341 tgaaaatagg gctaagggct cttgtaaaac agaaaatcta ttcatttatc aacatcctgg
     2401 gattaagcgt cggcatagcc agttgcgcgc taatacttat gcatgtggaa gatgaatttt
     2461 cttatgatca gtttcatctg aaaaagaatc aattatataa aattggtttg gaaagaaaat
     2521 atccaaatca tactacttac tttgatttaa tcccgcacag ttttatggag gtgatggttg
     2581 aggattatcc cgaagttgtg gccggtgcgc gcatagcaga ttttacaggt ggcggacaat
     2641 tcactttgat gatgcgttac attgatgaaa aaaataatga aaaagctttt gaggaatcca
     2701 gatttctggc tgctgattct aacatattcg atatgttcac cattgaagtg ctgaatggag
     2761 acaaagagaa agctttacgc ggagcaaaca aggtggtaat taccgaagag accgcgaaga
     2821 aatattttgg tgatgctgat ccgattggca aaacattatc tgctcctttt gcagaatttc
     2881 aggtaacggc ggtttgtgaa aatattccgg aaaactcaca ttttgatttt gattttcttg
     2941 ccagtctttc aagcggtggt aatttttttc aacagactaa ctttacgggc ttttcaacgc
     3001 acacctattt agaattagaa ccggacgcca atcctaaagc actggaagca aaatttcccg
     3061 cgatggtaga taaatacgcc ggcccgcaga ttgaacaaaa tttaagcatc gcctatgctg
     3121 attatgttgc cgctggaaat ggttatcgtt atatcatgcg acaattgacg gatgtacatc
     3181 tggatccaac gaactatgaa agtaagatga agcccggcgg taatctcacc tacgtttaca
     3241 ttttcatcag cattgccatt atgatcatga ttattgcctg cattaatttc atgaatctgg
     3301 ctaccgcgcg gtctgcagaa cgcgtaaaag aagtcggcat ccgcaaaacc atgggcagcc
     3361 accgtagaca actggtatcc caatttctta ctgaatctat tgtaattagc tttgtgagta
     3421 tgctctttgc atttttgatt atttatctgg ccctccccta tttcaatacg ctggctcaaa
     3481 agaatcttgc acttaacatc acgggaagta tgatagttcc tatgatgttc atttttgcac
     3541 ttatcgttgg attgctggca ggaagttatc ctgcctttgt tatttcgggc tatagcccgg
     3601 ttagtgttct aaaggggaag cttcactctt caggccgggg ttcctggttg agagatgggc
     3661 tggttatttt tcagtttttt gtgtccataa ttttgatagt aggaaccctg ggagtgaaaa
     3721 agcagatgga tttcatgcgg aataaatctc ttgggtttga taaagatcat gttatttacg
     3781 taaccggaac ccaggtactt ggtcagaact ttgaatcatt tgttaatgag atcaggagaa
     3841 tgccaggggt agaaagtgca gcaggagggc aatctgtacc gggtctattc acatttggcg
     3901 ctcagtttac caaagaagga gccactgaac cattaaccac caagagtttt aatatgtctg
     3961 atgattttgc tgaaaccctg gggtttgaga tcgtggatgg cagggatttc agtaaggatt
     4021 ttgatgattc actttctctg atattaaatg aggttgcagt aagaactttg ggtctggagg
     4081 atcccgttgg gagcaggctg tcaaccaatg caggtggagg gaacggtgga aatcgtattt
     4141 ttacggtggt gggggtcgtg aaagattatc atttccagtc tttaagggac gagattacac
     4201 cacttgcgat tttctgtacg gaatccaatg gtcctggtcc aaattttctg gttgcaatcc
     4261 ggacggagag cagaaacctg ggtgatctgg ttctttccgt tgaaaatctt tggaatcgcc
     4321 tcgcagttaa taacgaccca tttaagtata aatttctgga tgagtctctg gaagctcagt
     4381 acagggatga aaaaagatcg ggagaactct ttggcatttt tgccggtctg gcaataatca
     4441 ttgcctgtgt gggattgttt ggattagccg cttatactgc cggtttacga accaaagaaa
     4501 ttggagtaag aaaggtgatg ggtgcctcag ttgctcaaat agtagctatg cttagtatcg
     4561 aattcacaaa attgattttg atcgcgtttg tcctggccgt tccaatggcc tggtggggaa
     4621 tggacaattg gctcaatggg ttctactacc ggactacttt aggaataagc acgtttttta
     4681 tcgccggtgg ctccgcctta cttatcagct ggctaacggt aagctaccaa tctattaaag
     4741 ctgctatcgt aaatccggta aattcattgc gaagcgaata aaagcctatc gggtataatt
     4801 aagctgcacc aggccggaat caaaaacctt tgtttccttc aggataaatt tttttctttc
     4861 tcttaatgaa tggaacaaag gttttccttc accaaggata atgggatgca cagccagaga
     4921 catctcatcg atcaggttat tttgcagaaa gtgatgagtc aaatcagctc cgccgaataa
     4981 ccagatatcc ttaccttcag tttgcaaaat tgtatccacg gtagctttcc aattgccgcg
     5041 gattaattgt gcatctgcaa tttctttcaa agaattcgaa aaaacatatc gcttaaatcc
     5101 cgggaatgaa ttgggatcta ctttcagaag cagttcataa gttttacttc ccataaataa
     5161 actatctatt ctcttcataa aatcagacat tccgtagtct tgatcggtga aacaccaatc
     5221 atattcccca tttggacctg cgatcatgaa atcaaggctg accgctacgt taagaataac
     5281 cttcctcatt taggttgaaa agtttttgta tttttacatt tcggcaaaaa tggtttccag
     5341 taattttttt gtggctttaa ttccatcctc ttcgcttaat tttccacctt catactcaat
     5401 tcctatatat ccatcgtatc cggaatctgc tacaatggtc agcatcttgc gataatttgt
     5461 ttcgatgcaa tttccgttgt catcaaagtc ataactttta gcacttacac ccctggcaaa
     5521 aggcatcagt tcttccacac ctttatacct gtcataccat tcattattac ccaaattaaa
     5581 atttccgaag tcgggaagcg ttcctgccag ggggtgatca atcatctgga taacttccga
     5641 caaccatttt ccattggatg agagaccgcc atgattttcc actattacat tgatatcaga
     5701 tttttcagcg aattcgcaaa ggctgcgcag accctcagca gctaattttt gttgttcttc
     5761 aaaagacccg ctactacggg catttacccg gatggaatga caaccgagga attttgcagc
     5821 atcaatccag cggaaatgat tctctacaga ttccttgcgt tttgctgcat ccggatctcc
     5881 aagattacct tccaaatcac acattatcag gaggcttttt actccttccc catcggcccg
     5941 ccgctttaga tcggtaagat aattggtgtc acgagcgcgt gaaaaataaa aaacattcac
     6001 atactccaca gcatccaggc caaattccct ccgcgcaact accggaaaat ccagcggatc
     6061 cagatctccc agataaactg attttggatt gtttaacaat tgctccctca ggttgggctg
     6121 actgttgcca ttattgtaaa ttttcctgtg aagggaccac tgcgccaggg agattttaaa
     6181 tggagcctca atgtttttaa aagttagctt ctctgtagat ttagaagtta gcttctctgt
     6241 agaacctgct gataagtttt tcagaataag agaacttcca aggaatccgg agccggtaat
     6301 cataattgat ttttttattg cctgtcttct tttcatttca attaaagagg atttgacgat
     6361 cttcaattta gggtttttta tttttaattg agtgaaaata ataacaaatc cctatacttg
     6421 cgggcaatgg caaatacctt gaaaatagtc tcctggaatg tgaatggtat ccgggcaatt
     6481 gtaaaaaagg attttataaa atccatcaac agaatgaacc ctgatattct ttgtttgcag
     6541 gaaacaaagg ccccttatga ggaagtgaaa acagtttcca cgctgctaaa tcaatttcct
     6601 gaagttcata taaattcttc aaaggccaga aaagggtatt ccggtacggc catttttctt
     6661 aaaaattctt cagctgaagt taaggctgac atgggtattc agaagcacga ccaggaaggc
     6721 agggttttaa ctgcttcatt ccccggtttc ttcctggtaa cagtctatac tcctaattcc
     6781 ggccgtggcc ttgagcgatt gaaataccgg accacctggg acacagattt catgaaattc
     6841 ataaagaacc tggaaaaaga aaagccggtg attgtttgcg gtgatcttaa tgttgcccat
     6901 caggctattg accttgcaaa tccaaaaagt aactataaca aaaccgcagg ctatacccag
     6961 gatgaaattg atggtttttc caggttactt gaaagcggat ttgttgatat tttcaggaaa
     7021 cgaaatccag agctgctggg tgtttatacg tactggaatt atatgttcaa cgcaaggcaa
     7081 aataataagg gttggagaat tgattatttt ttgatttctg aatctttggt gcaagctgtt
     7141 gttgaagcag aagtctatcc ggaatttcat ggcagtgacc attgtccggt tggactggtg
     7201 attaatacct aattaataaa cccgaaccat tcctctccct cacgatgccg aaattcaatc
     7261 atccccggcc acaaaggtgc agccaacttt gaagcccagt ctgcgtgcag tacttttaac
     7321 cggttgacaa ggtcaggttg aacttcagca aaattccgtt cctcaaattt gtcagcggcc
     7381 atattaaata acaatgtctg gcctgtgagg tcattataaa tcaatttcca ttgctcatag
     7441 cggatggctt tattgtaacc accttgccaa aaaatacttt catgaggtgg gcttgttacc
     7501 tgcctggtga tgtatggcat taaatctttc ccatcaatag ttctttcctt cggaagaccc
     7561 gagccagtta aaatcgctat tgttgaaaaa atgtccaatg tggttgcagg aaagataaat
     7621 ttactccctt tttcgatttt acctttccat tgcatcatca tcggaatgtt tacaccgcct
     7681 tcaaattgag taattttacc ccctctgagt ggcgcattgt ccgtggtttt tgtatatgtt
     7741 gctcctccgt tatcactgag aaagataatt agggtatttg gtgcaagtcc aagtttatca
     7801 accgtggtag taattttgcc cacagcgtca tccaaagatt ttatcattgc gagatatacc
     7861 ctttttaccg gatcttcaat atgggaaaat tgttcaatgt atttcccggg tgcctgcaat
     7921 ggtgtgtgag gggcattaaa agggatatac aaaagaaaag gattgttttg gttttgatca
     7981 atgaacttta ttgcttcttc agcaagaaca tcggtcaaat atcctttctg ttcaaccagc
     8041 tcctcattgc gatacaggtt gcaatcagca ttgccaagac atccccaaat atactggtcg
     8101 gtccaatctt cttcgattcg ctgactaatg atgtcagggt tgtcttctct tgcatataaa
     8161 tggtgggaag atttgaatcc atagaaataa tcaaacccaa aattgcgtgg agacaattca
     8221 tcggcatatc ccaaatgcca tttcccgata agcgccgtct catatcccct ggcttttgct
     8281 aattcgccga aagtaatttc agagggtggc aatccctgct tattaatgtc ctcctgattc
     8341 ggaacagaat tcatcctgat aggataccac ggatcgctgt ccacaaaaaa tttcattcca
     8401 taatactgca taaggtttgt aagataagtt tcatggattt gaaactccat gccaaacctt
     8461 tgctggtatc gaccggtaag taaacctgcc cgcgaaggag tgcacacaga tgcagtcgtg
     8521 taggcttcgg aaaaagtaat gccattgatt ccaatactgt caataaatgg ggtgttgatt
     8581 aatttactgc catactttga aatatcagtc ttgcccaaat catctgccag gatgattatt
     8641 atattgggag ttatcgttga gtcatttcca gtttggatgc tcgtcagaaa tttatttttg
     8701 gcatcaagct gatcctgatc atgattaatc gcgaattttt tgcttgacaa cggataaaaa
     8761 aggtatgcga caaggattaa aattaaagac aaggttattc cgcgagtaat tttattcatc
     8821 cttgcaataa tccggctttt tggggattat tttaagaaaa aatgcaaaat gcgactggaa
     8881 attagttctt atccacctga atccagattt catcaactgg atttcccgtg gagccttttc
     8941 cgctatggtg ccaaccgtcc cctgttagct caatatcaaa ggttaaactc atacctaccc
     9001 tggttgagtc acgggaaaaa aactcaatat gttccgtata cttggcatcc tttacttcat
     9061 acgtgccgcc accggttcca aagaattctt ttgtttcaga attcagggca atccattgaa
     9121 atctgttttc agaaagaatt tttaaagttt tccttggccg atccggcatt tccacaaaat
     9181 tcccatcccg catccggcca ctgattctcc aggctccgct gagtggatat ttttctgtcg
     9241 cttcaagttt cttccaggtt tccatccatc gttttccttc ctttcttcca accttcctca
     9301 aggtgtttcc ggccaattca acttcatata cttcagcggt attcactagg tccgaattcc
     9361 aggtgttcga aagaaataca tcttcaagcc ggttcccgac gatcgtataa gtaccagccg
     9421 aagctccctt aaaatcttta tccatagtgt caaagctcgc ttccgaaaaa tagccgttgg
     9481 taattattct tactcttcgc tccgatgcaa cattgccatt ttgagacatt agctcccatg
     9541 aaccctgaag attcaaattt tctctttttt cgatttcact taatttgata aatgacgggg
     9601 aggtgaaaac cagcagcaaa aatattattg ttttcatcgg tttaggtaat tttcttcctt
     9661 taagttagtg taaattgttt tgaattccag agactttgag agttaagagt gagtttaggg
     9721 tttagggttt agagtttaga gttactacct aaaaggcagg gcttgaccat tccaatcggt
     9781 tgaacttatt cctggcgagg gtgctcctca actcttaact ctaaactctt aactctataa
     9841 tagctaatat gttaaatcag caaaaaacaa aaaataccac gatagcttta aaaggcattc
     9901 cttttgatgc gaattcttcg tatttacctg gggctgcaaa gggaccaggg gcaatccgga
     9961 aagccctgct tagtccgtca gccaatcttt caactgaatc aataattgat ttaggttcat
    10021 cctgccagat aattgatgcc ggagatattc aggttagaga tttctttcct gatatcgttc
    10081 aagctacaag ggaaatttta tcatcgaatt tatcgcctgt tattttgggc ggtgatcata
    10141 gtatcaccta tccggtgatt aaagcctttt ctgagaaatt tcctgactta accatcctgc
    10201 atcttgatgc tcacggggat ctttataatg attttgaagg aaacaaattt tcccatgcca
    10261 gcccctttgc ccgaataatg gaagaagggc tcgccacacg gttaattcaa gtgggaataa
    10321 gaaccttgac accacatcaa aaaatacaag ctgaccgttt tggtgtggaa attattgaaa
    10381 tgaaacattt tcagatcaga agcatacctg aactggcatc acccttgtat ctttcgctgg
    10441 acatggatgt tttggatcct gcatttgccc cgggaatttc gcattacgaa cccggaggaa
    10501 tgagtacccg gcaagttatt tccattttgc aatccattaa gccaacctta gtcggtgccg
    10561 atattgtaga attaaatccg gatcgggact tcaatgaaat gacagcgatg accgcagcta
    10621 aattcttaaa agaaattatt gacaacatgc ttcgaaaacc atgaattttg atctgaataa
    10681 aagcctggaa atcttaaaaa gaacacctga tgtgctggat gctcaattaa aggaccttcc
    10741 cgcgttctgg tctgaaggta aagaagcggt tggccggtgg tcagccatgg atgtcgttgc
    10801 acatctgatt cacggtgaaa tcgaagactg gatacccagg gccaaaatta ttctgtttga
    10861 aaaaaataag cagtttccac catttaatcc ttcaggattt ggtaagattc ttcaaaataa
    10921 aacgatggat aaactgttga acgatttcag gaaattgaga gaatctaata ttagagagct
    10981 aaaaaaatta aatttgtccg aaaatgattt tcagaaaacc ggcattcatc ctgaatttgg
    11041 tgaagtaact ttgcatcagc atttatcaac atggactgtg cacgacctga atcacttgta
    11101 tcaaataagt cgtgcggttg gaaaacaata ttctgaagat attggtccgt ggaaaaaata
    11161 tttaagatta gtaagagact aacatgtgcc tggttgtatt ttcatacaag aaacatctta
    11221 attataaact gatcatcgcc gctaaccggg atgaatttta ccatcgtcct acgcagccgg
    11281 cagacttctg gcatgattct cccaatctac ttggaggaag agacctggaa gcaaatggaa
    11341 catggatggg ggtaacaact gaaggcaaag ttagtctgct cactaactat agagacctac
    11401 gaaacctcaa acaaaataca ccgagtcgcg gccaccttgt ttctgatttt ttgaaacaag
    11461 aagagcttcc taagacctat cttgaaaaat tacacaacat atcggatacc tataacggat
    11521 ttaacctggt agttggaaat caggatgacc tgtggtatta ttctaatgag aacaaacgga
    11581 taacaatgct aggttccggg ctgtatggtt taagtaatgc tctgattgat actccatggc
    11641 caaaattaat cagggcaaaa aataaaattg gagatgcctt taatgatgac tttattaatg
    11701 cagataaagt tctcgacgca ctctttgatg acctgagagc aagtgatgac cagctgccgg
    11761 acacaggcat cggttttgag aaagagaaaa tgctctcctc cattttcatt aagagtcctg
    11821 attatggaac acgctgctct acattggtta cagttgatca aaatgacaac ctgaactttc
    11881 atgagcgaac ctatgatttg aaaacctttg catattcaac ccggcttttc gaattccggt
    11941 taaattccag ggtacgtggc tgaaaaaaaa atcaacgtgc cttttattct ccggttcatc
    12001 ccctggatct ttccaaaact ggagagaata gtgccgctcc tggcatacaa atttgcggtt
    12061 aaacttttct tcaccccaat ccggtacaaa attccttcca cagaaaaaga atttacgaaa
    12121 acaggcgagc cttttctgat tgaagtaaac cggaagaaaa tccggggctt tagctggggt
    12181 gaaggaaaca cgattgttgg agttcatggg tggtcaggac gcggagctat gttgcgcaaa
    12241 tttgttaaac cgttaacagt aagtggtttc agggtggttt tgttcgatgg acccgcgcat
    12301 ggctcctctg aaggcttcag caccgatcca ttagagtttg aaggagtcat tcttgaaatt
    12361 gaatcgaaat tcaatccggt ggttggctac atcacccata gtttcggggg tacagccgca
    12421 ttgttttgct tttcaagagg gctgaaacca aaacccattg ttctcatcgc agttcctgca
    12481 attggcgagg atgtggttca gacctttact gcgcgaatta acggaacccg taaagtgata
    12541 aagaagctcg aagaatttac acagcagcgg tatggcaaat ctttctatga gataagcgca
    12601 ataaatacag cccggactat tggattcacg ccagctctcg taatccatga taagaatgat
    12661 gacgatgttc cggttgaaca tggggaaaag cttatggatg ccctgccaaa tggtacgctg
    12721 ttgcgaacta tagggcttgg ccacaataga attctcagag atccttctgt tgttaaccac
    12781 gtggttgagt tcttcgcaca tcattgcagt ttaataactc actaaattgt cattaattat
    12841 tacctttgtt ttcctcaaaa attcgctgag atggagatta agaaaattac tttgaatgat
    12901 gtacatgaaa gcctcggcgc caaaatggtt ccgttcgccg gattcaatat gccggtgaga
    12961 tatagttctg atatcgaaga acacatgcaa gtcagaaatg gagtgggcat ctttgatgtt
    13021 tcccacatgg gtgaattttt tctcagaggc cccggggctt tggatcttat ccagaaaatc
    13081 agttccaatg atgcgacaaa attatttccc ggaagagctc aatattcctg tttgccaaat
    13141 aagattggcg gaattgtgga tgacttaatt atttatatgc tgaaagaaaa tgaatacatg
    13201 ctggtagtta atgcgtcaaa tattgaaaaa gactgggatt ggatttccag ctgcaatgat
    13261 tcaggcgttg acatggaaaa tgcttctgat aaaatatctt tatttgccat ccagggtcca
    13321 aaagcgattg aagcattgca gcctttaagt tcggtcgatc tttctgctat caagttctac
    13381 cattttgaaa ttggcgattt cgccggcata caggatgtga taatctccgg cacaggatat
    13441 actggatccg gtggttttga aatttatgtt aagaatgaag atgctttgca tacctggaat
    13501 aaggtaatgg aaacggctgc tccttttgga attaaaccaa ttggtcttgg tgcaagagat
    13561 accttacgtt tggaaaaagg ttattgcctt tatggcaatg acattgatga taccacctcg
    13621 ccattggaag ccggtttagg ttgggtaacc aggttcacaa aggaatttat aaattccggg
    13681 aatctcaaag aacaaaaaga aaaaggagta gaaaaaaaac tggctggatt tcaaatgact
    13741 gatcgtggaa tacccaggca cggctacctg ataaaatcag aggatggaaa tactattggc
    13801 cacgtcactt caggaactca gtctccaatg ttagccgttg gaatcgggat gggttatgta
    13861 aaggatgact actcaaaacc aggttcaata atatttattg aaattcgaaa taaattattg
    13921 aaggccgaag tgaaatcttt accctttgtc taaatgccat ctcttgaaat ttgttttagt
    13981 cctgacttac tgcatttgta tactttggaa aacaagaaag tggtggttgc agacatcctg
    14041 cgcgcaactt cctgtatggt taccggcctt gcctgcgggg taaagtcttt tactcctttt
    14101 gctgatttgg aggaatgccg gcaaatgagg caaaaaggat acttgatcgc tgccgaaaga
    14161 gacggccaaa ctatagatgg ctttgatttg gggaacagtc cattctctta catgagtgat
    14221 gaagtggctg ggaaaaaagt agcatttacc accacaaatg gcacccaggc catacaaaaa
    14281 tccggaaaag gcgaaattct aattggttcc tttttgaatt tgcaagctgt gacagatttc
    14341 cttaatgctt caggggaaga tgtcgtagta gtttgtgcag gatggaaagg gagatttaat
    14401 cttgaagata cgttgtttgc cggggccttg tcaatgaatc ttgtaaacta tagcattaac
    14461 gatgatgcgg cgcttgcagc taagtacttg tattccattt ccaaaaataa tctctttcaa
    14521 ttcctttcca attcttctca ctttagccgc ctaaagaaac taaatattca aaaagatatt
    14581 gaattttgtc tttccctaaa tcaatattct aatttaccaa aagtaattaa tggcattgta
    14641 acaaataatt atgaaggaaa taaccccctg tagtccccct taaaacaagg gggacaattt
    14701 ctggtagggg gtactttccc ctccttaatt taaggagtgg tctgccgacc gaaggttggt
    14761 taggggtggt tactaaaata gtagtttaaa ccccctgtag tcccccttaa aacaaggggg
    14821 acaatttctg ggcagggggt actttcccct ccttaattta aggagggcct gccgaccgaa
    14881 ggttgggcct gccgaccgaa ggttgggcct gccgaccgaa ggttgggcct gccgaccgaa
    14941 ggttgggcct gccgaccgaa ggttgggcta ggggtggtaa aatagaaatg cagttgttaa
    15001 aaccccctga agttcccctt aaaacaaggg agacaatttt ccaggcaggg gtggttcaaa
    15061 ccccctgtat ttttccaggt gcagttgtta aaaccccctg tagtccccct taaaacaagg
    15121 gggacaattt ctgacagagc tgatgtattt gcggtaattc aaaacc
//
LOCUS       DASWHX010000030        47980 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_762872, whole genome shotgun sequence.
ACCESSION   DASWHX010000030 DASWHX010000000
VERSION     DASWHX010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 47980)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47980)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47980
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_762872"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..882)
                     /locus_tag="VGA21_01610"
     CDS             complement(<1..882)
                     /locus_tag="VGA21_01610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649231.1"
                     /translation="MAQQTPVQVSAFLNPPYSLALYEYGMPGSNLLQVQLLLTDLTKN
                     QYPVKLRLIVEGVGITIKTKSTFLPQPIPLSGGTPYILTAEDLQPYFSLENLDLLGIS
                     KRDYQRLGMLPEGFYRFTIQVLDYYRSSVVSNSAMAQAWIILADPPFLNYPMKEEKLV
                     ARDPQNIQFSWSPRQKSPNMGLTEYTLQLYEILPEGSNPYNIAASTQPYFETTTFNTA
                     YFYTLSDPMLVPGRQYAWRVKAANTEGYDRYKNDGYSEVRTFRWGDACEEMLNVTLTP
                     YNNHIRIDWDPQFGHTSY"
     gene            complement(1122..2345)
                     /locus_tag="VGA21_01615"
     CDS             complement(1122..2345)
                     /locus_tag="VGA21_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009579566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HEX9649232.1"
                     /translation="MTRMKKSGLEELVKGSLYPQEQLEKVKSGKNSFYIGIPKEISFQ
                     ENRVSLTPDAVELLTNNGHRIRVERGAGLKSKFTDHEYSEAGAEIVNTPKEAYDAQIL
                     LKIEPPTAKEMDYLGPGKVLLSALQLGNQDAEYIKTLMSKRITAIAFEFIEDKVGGFP
                     IVRAMSEIAGSTVMLIAAEYLSSVNDGRGVILGGITGVPPTQVVIVGAGTVAEYAART
                     ALGLGADIQIFDNHIHKLRRIRHALGQQVYTSTVDTKTLSDSLKNADVVIGAVRLDKN
                     QRRILITEEMVMNMKPNSVIIDCSIDQGGCVETSRITTHKNPVFRKYDVIHYCVPNIP
                     SRVARTATTALSNIFTPTILRAAEDGGIEDMIFTHKWFMKGVYIYNGSLTNEILGKKF
                     KMKHKDLNLIMAARF"
     gene            complement(2338..2781)
                     /gene="tsaE"
                     /locus_tag="VGA21_01620"
     CDS             complement(2338..2781)
                     /gene="tsaE"
                     /locus_tag="VGA21_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009579567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEX9649233.1"
                     /translation="MVEKAAGELEIKYHSINELPSISKEIIDFAENIKVWLFYGEMGI
                     GKTTLIKEICSSLSVKGSVTSPTFNIVNEYFISDSKKINHFDFYRIENEIEAMDIGYE
                     EYFYSGNLCLIEWPDQIPNLLPEVYLKIVMSKESDLRKIQITRND"
     gene            complement(2774..4333)
                     /locus_tag="VGA21_01625"
     CDS             complement(2774..4333)
                     /locus_tag="VGA21_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013455648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional response regulator/alkaline
                     phosphatase family protein"
                     /protein_id="HEX9649234.1"
                     /translation="MQRYNILWADDEIDLLKAHILFLDKKGYNVTPVNSGADAIDMCE
                     KQNFDVVFLDENMPGMSGLQALNHIKSSKPNLPVVMITKSEEEHIMEEAIGAKIADYL
                     IKPLNPNQILLSVKKILDNKRLITERTNISYQQEFRSIASAFNSELSHEEWIDIYKKL
                     VFYELAINSSDNRDMAEVFDMQKTEANSNFVRFVSQNYESWLNDPASDKPLLSHQLMK
                     KKVFPYLDKDRPIFFLVIDNLRYDQWKILEPVIQEYFTIEEEDYYYSILPTTTAYARN
                     AIFSGMLPLEMANTYPQFWVGEDVEDGKNNFESEFLEKQLARNNLDLKASYVKIKHVN
                     QGKQLVDTIPNLRANQLNAIVYNFVDMLSHARTDMAMIRELAPDESAYRSLTLSWFLH
                     SPLLESLKKIAEMNARLIITTDHGTIRVKKPFKIIGDKNTNTNLRYKQGKNLGYDGRD
                     ILVCKKPEAFHLPKINVSTSYVFAVEDHFFAYPNNYNYYVNYYKDTFQHGGVSLEEMI
                     IPIISLNSKNG"
     gene            4406..5632
                     /locus_tag="VGA21_01630"
     CDS             4406..5632
                     /locus_tag="VGA21_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009579569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEX9649235.1"
                     /translation="MNKKKIINDPVLGFITINSDLILKLIDHPYLQRLRRIKQLGLTE
                     FVYPSAVHTRFHHALGAMHLMELALNSLRSKGIKITDDEYEAALTAILLHDIGHGPLS
                     HTLEYSLTERVEHEMITVLIMQKLDQEFGGRLKLSIRIFKGDYERNFFHQLVAGHFDI
                     DRMDYLKRDSFYTGVSEGNVGVSRIIHMLNVVDNELVVDEKGIYNIENFLIARRFMYC
                     QVYLHKTTVAAEKMMENLFARARFLLKSNSALVVPPALKPFLVKRISANDFQKDNLVL
                     ENFLNLDDSDIWEALKNWKSSSDKILSDLSNWILNRRLFTIEMSGAPFDAALIDNAQK
                     KISEVFEIDNPATQYYFSHGLLFNSAYGGHYGPIKVLTKKGLILLEDLSEVPAISELS
                     KIVRKYYLCYPKNVSL"
     gene            5667..6713
                     /gene="lpxD"
                     /locus_tag="VGA21_01635"
     CDS             5667..6713
                     /gene="lpxD"
                     /locus_tag="VGA21_01635"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009579570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HEX9649236.1"
                     /translation="MEFSIEEIAVIVGGEIEGNGSDTINKLSRIQDAKEGSISFLANL
                     KYEPFIYKTEATAIIVGTEFKARKKLNVTLIRVEDPYVGFTLLLEEYHRLISLQREGV
                     EEPSYLGNNTTIGEKIYRGAFSYIGSNVKIGDNVKIYPQAYIGDNTTVGNNTIIHSGV
                     KIYPGTKIGTDVVLHAGAVIGSDGFGFAPQDDGSYKTIPQMGNVIIHDKVSIGANTTI
                     DCATLINDSTEIGEGTKLDNLIMIAHNVHIGKNTVIAAQSGVSGSTRVGDNCVVAGQA
                     GIIGHLEIANNTTIAAQSGVGKSIKEPGQVLMGSPGFDHKQYLRVYTVFKKLPDLESR
                     IKELEQKVLNLPTI"
     gene            6721..8115
                     /locus_tag="VGA21_01640"
     CDS             6721..8115
                     /locus_tag="VGA21_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010609286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase/3-hydroxyacyl-ACP
                     dehydratase"
                     /protein_id="HEX9649237.1"
                     /translation="MYTNQHTIRKSVTLSGVGLHTGVQANMTFVPAPVNHGIKFQRVD
                     LEGQPIVEADVDNVVDLSRGTTIEKNGARVNTVEHTLAALVGLQIDNILIQLDGPEPP
                     IMDGSSKPFVEILRDAGCIDQKTPRNFFEVQESIFYRDGSRDVEIAALPLDDYRLTVM
                     IDYKSPVLGSQHASLNDISQFDKEIASCRTFCFLHELEMLHKNNLIKGGDLNNAIVVV
                     DRVVEDHELESLAKLFNKPKVEVKKEGILNNVELRYKNEPARHKLLDVIGDLALAGRP
                     IKAQILAARPGHAANVAFARKLKKAMQAAKSHEVPKFDPNSPAVVDINNILTTLPHRF
                     PFLMVDKIFHLDEKSVAGIKNVTINEPYFQGHFPGNPVMPGVLQVEAMVQVGGILVLN
                     TVSDPENYWTYFLAIENFRFRKMIRPGDSLIIKCELLSPIKRGIAKMEGRAYVGNTLV
                     CDGVMTASIVRKDS"
     gene            8112..8891
                     /gene="lpxA"
                     /locus_tag="VGA21_01645"
     CDS             8112..8891
                     /gene="lpxA"
                     /locus_tag="VGA21_01645"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009579572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HEX9649238.1"
                     /translation="MNQPLAYIHPSAKIARNVVIEPFTVIHQNVEIDEGTWIGSNVTI
                     FEGARIGKNCKIYPGAVISAIPQDLKFGGEDTTTEIGDNTVIREFVTISRGTVDRYKT
                     VIGANNLLMAYVHVAHDCQIGNNCILANAVQLAGHVQIDDHAIIGGASAVHQFVKIGA
                     HVMISGGSLVRKDVPPYTKAAREPLTYCGINSIGLRRRGFESEKITEIQEIYRYIFLK
                     GHNNSDALDFVEIDLPPSKERDEIIRFIRNSDRGVMKGYSH"
     gene            8891..9508
                     /locus_tag="VGA21_01650"
     CDS             8891..9508
                     /locus_tag="VGA21_01650"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX9649239.1"
                     /translation="MNIEVSNLGKSFNHHRIFHNLTYTFQQGQSYSLLGPNGSGKSTL
                     LKILSGFMLASNGIITYKSRDKIIDPESWYKYQFIAAPYMELPEELTLSEFLRFHFKF
                     KKFSPGYEFDNAISKCGLQGKDEVQIKNFSSGMKQRLKLALGMYSSQEILLMDEPATN
                     LDQSGVEWYLREIKGILPEKLVIIASNTLIEHDFCQNCLNLNELK"
     gene            9618..10082
                     /locus_tag="VGA21_01655"
     CDS             9618..10082
                     /locus_tag="VGA21_01655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649240.1"
                     /translation="MKIVRKASILLMVLAVLLVFGCKKKKDIEPDPEAEAIAKLVSGA
                     WVPTAVTLDSENRTADFTNFSLTITENKTYSTLGVDPIYSSVWPSSGSWDFKSSGSAI
                     PDLTTIIRDGSLEISIDVLSETQFVMSFTFVTGSVEGTSSTEGNYIFSMEHN"
     gene            complement(10182..12530)
                     /locus_tag="VGA21_01660"
     CDS             complement(10182..12530)
                     /locus_tag="VGA21_01660"
                     /inference="COORDINATES: protein motif:HMM:TIGR01069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HEX9649241.1"
                     /translation="MTSAINDIEQSLGFSGIRELLKEFCLFEPGRELTNQMKWETDPA
                     LVVCSLGLDEEFLEILQGPAAFPVSNYYDPRVSIGKLKPQGTFLEEDEVWEIYLFLKT
                     CDEIDKFLMVSKEDWPTLNSLRLENYPRTLIKAIQLKFDETGKLRDNASPELNKIRKR
                     KKEVEGSLGKVINRIYQEASLNGFVPKEMSVSVRGGRVVIPISSEHKRKIRGIIHDYS
                     ATGSIVFIEPDSILEITNQVQNLQHQEKIEIIKILLKISDEIRAEAGFLLKAVQFLAT
                     IDFTRSKALFAKKIGAVKPAVDLHEPMKIVNGRHPLLLLQNKEAVPLNLVIDKKERIV
                     MISGPNAGGKSVALKTVGLFQYMLQCGLLVPAEETSSFKIFNKIFLDIGDQQSLENDL
                     STYSSHLKNMSEILANADEKSLILIDEFGSGTDPQIGGAIAEAILEGIIQRKCWGLIT
                     THYSNLKHFAEKSEYLSNAAMLFDKQNLRPFYILEQGRQGGSFALEVAQKTGLPEEII
                     ARAKELAGSEAVSLEDILARLDQERKELSNRLLQLDKSEREAALNKEMYLQLKKEIEG
                     EQRELINDASMKAETLLNEANREIEKTIRHIKENKAEKKETRKIRNQLDEFRDKVKVE
                     PEKSGEFPGKDFFCLNDTVEINKEGMVGEILEIRGKEALVLVGNIKTKVKLKDLRKTD
                     AKPLTPRPASGYQFERKVQQLSTSLDVRGLRAEEVIFQVDKFLDNALVGGINQVSVIH
                     GKGNGILRKVIREHIKRLSHIQKFENEHLERGGDGVTVIYLK"
     gene            complement(12514..12879)
                     /locus_tag="VGA21_01665"
     CDS             complement(12514..12879)
                     /locus_tag="VGA21_01665"
                     /inference="COORDINATES: protein motif:HMM:NF025494.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4296 domain-containing protein"
                     /protein_id="HEX9649242.1"
                     /translation="MKFYLAIVCVFTGCVSEVEIPKDVMSKNQMISFVIDVHILEAQT
                     GQLRISKDSARVVFQYFEDELLQKHKVNDSLYYRSLEFYYAHPLLMEEVYAAVLDSLS
                     LYERMSKGNLETEGSDQRN"
     gene            12938..13819
                     /locus_tag="VGA21_01670"
     CDS             12938..13819
                     /locus_tag="VGA21_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017731814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX9649243.1"
                     /translation="MKELVKKLRKYEIQIRKVVDSQRQGDFHSIFKGSGLEFDDVRDY
                     QYGDEIRTIDWNVTAKGHGTFVKTFKEEKEQTVFFVLDVSGSQEIGSPGHQKMDIGKE
                     ICGVLSLAAIKEHSQVGLLCFSNQKEVYIRPSKGSRQAYAIISSLYHLKPESGKTDIN
                     KAIVFTLNTIKRKSVVIFISDFIDKNYANNLKALAKKHDLVIIQIGDKRESELPKLGI
                     VPVYEKESKTTTWVNTSSHFFRSNLQDTYLQNTENLIRFCRKNQANYLYIDTQEDFVP
                     KLIRLFKIRNKTRKSAR"
     gene            13809..14693
                     /locus_tag="VGA21_01675"
     CDS             13809..14693
                     /locus_tag="VGA21_01675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649244.1"
                     /translation="MRDRIGVLMLLIFISTGASAWQVTPKGKFLSDSTRIGVELLYSL
                     SVTYPLESDIVFPDSLYDFSPFELNQKDYFFTKSDNFFSFDSAVYNLSTFEIDTFQML
                     ALPIWVIGNGDSIAYFPAPDTVILNHIVTQMPDSLALIDNTIYKEVNYAFNYPYFLIS
                     IGIASIVIFVIIYFYGKSIRRAYYLYKMKRSHERFVKAFNTTIHSNKHNWEKSLLEWK
                     KYMEQLDEGPYTRLTTREIARKTKDKLLLNALKGIDRVIYGNYERDTGKQYFEDLIHF
                     ARNAYANRVKQIKYGTGS"
     gene            14677..15726
                     /locus_tag="VGA21_01680"
     CDS             14677..15726
                     /locus_tag="VGA21_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013452558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX9649245.1"
                     /translation="MEQEVENIVWYSAEWFSPGIFQNYSWENQIALYLLFLIPIIIIV
                     RWLVRRKFSQKLPVSIPKENIKWNPITLLRFIPNILLAFIFTLLIIALARPQKTNEKV
                     EQWTEGIDIMIVVDISESMQIEDFQPNRLEAAKNVANEFIDGRFQDRIGMVIFSGDAY
                     SLSPLTTDYKLLKQYIKEISFDMIENRGTAIGSALAVATNRMRESDSKSKVVILLSDG
                     DNTAGNIDPITAAKLAQAYGIKIYTIAIGKDGRVPFGKDFFGRTRYVENTLDESTLRE
                     IAAIGDGQFFRVSDNQALQQVFSLIDKLEKAEIKETRFKDTTDFYRIYLQWAIVMVLA
                     WMALKSSFIFNILED"
     gene            complement(15723..16337)
                     /locus_tag="VGA21_01685"
     CDS             complement(15723..16337)
                     /locus_tag="VGA21_01685"
                     /inference="COORDINATES: protein motif:HMM:NF013933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HEX9649246.1"
                     /translation="MDIILKGLGFGIILSFAIGPVFFSLIQTSVEKGFIAGLVMALGI
                     SISDTSFVLFSYLGFSKLISLPEYKYYLGIIGGIILIALGFYSFMKKKSYRQKSFVKN
                     PKSVIRALVKGFVMNTVSPFVLIFWLGVMGFATVDLGFHDVDLKIFFGTILLSVLVTD
                     VTKAYMANKLGLLIKPRIIEIMNIVVGVILLGFGIRLLYYAWTL"
     gene            complement(16337..17890)
                     /locus_tag="VGA21_01690"
     CDS             complement(16337..17890)
                     /locus_tag="VGA21_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009579582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HEX9649247.1"
                     /translation="MPLVGNLLQKGIRIRESLDQEYSSPIDLQKTELKKLLITASATS
                     FGKTYDFKEILQSFRSRERQMFYNVFKSKVPYHNYDKMFEVWWYKCLKGEKNVCWPGR
                     VKYFALSSGTSGAPSKHIPITDEMSKAIRKTSLRQILTLSRYDLPAQLFEKGILMVGG
                     SISLNYNGTYFEGDLSGIQASQLPFWFQHFYKPGKKIAKNPNWTEKLEEITRKAPEWD
                     IGIIVGVPAWIQILMEKIINHYKVKNIHEIWPNLMIYVHGGVFFEPYRKGFERLLAKP
                     ISYIETYLASEGFLAFQALPHRKSMRLVLNNGIFYEFVPFTDRNFTTEGELREDHEAM
                     MIDDVKENTDYAVLLSTCAGAWRYLIGDTIQFVSVEESEIRITGRTKHYLSLCGEHLS
                     VDNMNHAIKIISNELDIEIKEFSVAGVPVGSLFGHHWFIGCDDPVKSDVLAKRLDEVL
                     KELNDDYKVERNAALKSVEFEILPSAFFYNWLKTQGKEGGQNKFPRVLNKSSYNSWTM
                     YLQNEKVRR"
     gene            17993..18655
                     /locus_tag="VGA21_01695"
     CDS             17993..18655
                     /locus_tag="VGA21_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009579583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEX9649248.1"
                     /translation="MNINKNIAYFQNLTKTKHCKLIAVSKMKSEQDIMHAYDAGQRIF
                     GENRAQELASKYEKLPKDIQWHMIGHLQTNKVKIIAPFVSMIESVDSIKLCREINKEA
                     MKVNRVIPILLQVHIAMEETKFGFNSEELLEAIGDNTIKQFENVELKGLMAMATLTDD
                     NETIRKEFRFLKNLFDKVASLQVFPKFTEISMGMTSDYQIALEEGSTMIRIGSAIFGE
                     RK"
     gene            18656..19024
                     /locus_tag="VGA21_01700"
     CDS             18656..19024
                     /locus_tag="VGA21_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018473040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HEX9649249.1"
                     /translation="MASKAIIIGSILGALAVAAGAFGAHALSEFLNESGRSVTYETAV
                     KYQFYHSLLLILAGILGQKVSINIPVYLIVFGILVFSGSLYLLIFTNASWFGIIAPFG
                     GASLIAGWLSLAYAARKLVN"
     gene            complement(19017..19457)
                     /locus_tag="VGA21_01705"
     CDS             complement(19017..19457)
                     /locus_tag="VGA21_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009195556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HEX9649250.1"
                     /translation="MKKLMMTMFFLLTATIGFSQTAEQKKEQEQKETQEKAPGPVIAF
                     EIESHDFGDIEQGDIVEWTFKFENKGDEPLILSNVAVTCGCTATSWPREPVGVGEKGE
                     LTVKFNSVGKSGAQNKVITIYSNARIPISRVSIVTNVLPKKKVN"
     gene            complement(19513..20904)
                     /locus_tag="VGA21_01710"
     CDS             complement(19513..20904)
                     /locus_tag="VGA21_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013453042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX9649251.1"
                     /translation="MPNPSSILAQKFTHTPTQGQQKLFSLFDSFLEETKTKDCILLRG
                     YAGTGKTSLVSAIVKTLPLFNYKFVLLAPTGRAAKVLSGYARKVAYTIHKKIYKQAAD
                     PVSGLLKFELQKNYHKKTVFIIDESSMLYENNEFGDNGILSDLIKYIFNKKGNKLMLI
                     GDSAQLPPVGQSISAALDMDYLKLKCKLDVQEIELQEVMRQDINSGIMHNATVLRESL
                     SAKEINIRFRLKGFKDIFKMTSEKLEAGLRYAYDKYGVENTTVICRSNRAATQYNQFI
                     RRQIFFKEEEIETGDFLMIVKNNYFYKENPAGFMANGDFVEVMKVITFEDLYRYRFAT
                     LRLRMIDYPDEEEFEAKVLLNTLHTENTSLSPEENKKFYQEILKDYIQYSEIERAKAL
                     RFDPYLNALQIKFSYALTCHKSQGGQWPLVFVDQGYLTEEMINEDYLRWLYTALTRST
                     KEVYLLNFNSRFF"
     gene            complement(20916..21353)
                     /locus_tag="VGA21_01715"
     CDS             complement(20916..21353)
                     /locus_tag="VGA21_01715"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018477767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX9649252.1"
                     /translation="MIKVQNPEFESRIKKFLENQHFMKFVGFELDVIEPGRTEGWMEI
                     DQNHLQQTGLIHGGLITTIADIVAGFAAYSVVPATHHVVTGEIKVSFFKPGRGSRLFA
                     IGWVVNQGRKINFCEAEVYSENNGKKELIAKATTSMVVIPPKE"
     gene            21407..21901
                     /locus_tag="VGA21_01720"
     CDS             21407..21901
                     /locus_tag="VGA21_01720"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX9649253.1"
                     /translation="MVQFEESYHGKTRVRVCGLCLIENQLVLVRHCGLGETGTYWSPP
                     GGGTNFQEPITEALKREFKEETNLEIEVRKYLFIHEFIGARLHAIEIFFDVFRTGGNL
                     ILGEDPEHRKDNQWLQEVKLFSQDELSDIPQNEKHQIFSMVSNFKDLLSLRGFFKSEN
                     NSIK"
     gene            21959..22549
                     /locus_tag="VGA21_01725"
     CDS             21959..22549
                     /locus_tag="VGA21_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013453045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649254.1"
                     /translation="MNLTRILSISFLVVALALFAFLVNRIKFAIDEEIRIENVEKIVI
                     RKLEMIRVAQIAYQSVNGQYANTWEKLVDFVNTGDFYIIQRSEEIITLSYGRDSVIVH
                     LDTLGTVPVIDSLFKKQKYADFTLADLPIIPESGGKKFDLFTEKITKGGITVDVIEVR
                     DIAPANPMRRESNEARNRKPLRFGSRTDVTTSGNWE"
     gene            22554..23441
                     /locus_tag="VGA21_01730"
     CDS             22554..23441
                     /locus_tag="VGA21_01730"
                     /inference="COORDINATES: protein motif:HMM:NF024271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3822 family protein"
                     /protein_id="HEX9649255.1"
                     /translation="MEHTAIFYKPIHKVKDAKLSIDDLHHYSLSVQMGARDFQLCVID
                     TRDNRCLAMEDFALSKVRSYKQLTEVLNNLFDDHQFLNAGFWKSVKFSLKNSKFSLVP
                     DKLFDRNNSYDYLNVSCTVNPDKETFLYYKHIKSKAVNSFAIPTDLFNWINKLYKNSS
                     VLITHQSSALIEGVLRQVVKYSGPTIYLFIDRFKLHIITSKNGELEYYNQFIISRFSE
                     YMKYIMMVMQGLKYNQKNTEIVLWGYLGRKSPHFTEFSKFIKNISFGDRPDYLKFGYL
                     FDDLQDHHYFDLYSTYLCD"
     gene            23441..23893
                     /gene="coaD"
                     /locus_tag="VGA21_01735"
     CDS             23441..23893
                     /gene="coaD"
                     /locus_tag="VGA21_01735"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009186739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEX9649256.1"
                     /translation="MKRIALFPGSFDPFTKGHYDIVIRGLSLFDEIIIAIGHNTQKVR
                     YFKLELMVKKIKETFANNNRVTVLVYNELTAELAQKQGARYLLRGLRNTTDFEYENTI
                     SQVNRHLNSELESVFLITSPEFAALSSSIIREVHKYGGDVSELLPYPI"
     gene            complement(23881..24561)
                     /locus_tag="VGA21_01740"
     CDS             complement(23881..24561)
                     /locus_tag="VGA21_01740"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEX9649257.1"
                     /translation="MRIFINDVPVRVIKSKTFKKELEFEMDLNALENNLSNAQFFGNV
                     IIRNVSEKQLRKILKILTNSPFAKLNSVTIVVDFELRVKEVVKSYYTFINAAGGIVEK
                     GSKILMIHRLRRWDLPKGKVEKGESFMEAAMREVEEECNISVSPRKRICASYHTYSLG
                     EKNILKKTVWYSMKCIEDTHMKPAKEENIDEIRWMNHKELLHSLLHSYRSLGYVMECY
                     FRWKGQIG"
     gene            24649..25287
                     /gene="pyrE"
                     /locus_tag="VGA21_01745"
     CDS             24649..25287
                     /gene="pyrE"
                     /locus_tag="VGA21_01745"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008201620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEX9649258.1"
                     /translation="MNNAEAGRVIAATLLEIGAIKLNLKKPFLWSSGWKSPIYCDNRL
                     SLSFPASRTFIKEHFGQLITSKFPDAEVVAGVATAGIPQGALVADLLNLPFIYVRSKP
                     KGHGMENLIEGKVEKGQKVVVVEDLISTGKSSLNSVDALRKAGLQVLGLVAIFTYGFP
                     LADTNFKEANVQAFALSNYTYLLEEALDKNYITNDQLVSLNGWRVDPEKWEP"
     gene            complement(25284..26066)
                     /locus_tag="VGA21_01750"
     CDS             complement(25284..26066)
                     /locus_tag="VGA21_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017731797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02757 family protein"
                     /protein_id="HEX9649259.1"
                     /translation="MAQKNLLDLKNFLDLKVEEYNNPKFIGSDPISIPHLYRKKQDIE
                     ITALWTAILSWGQRKTIINKCKELFSLMDDAPYEFIINHSQSDLKRFRNFKHRTFNYT
                     DTLYFISFFKWYYSQNKSLENLFVVKESDETIQNGLANFHNTFCSLPNFPSRTRKHIS
                     TPVRNSACKRLNMFLRWMVRKDSNEVDFGIWRKISPSKLICPCDIHVENVAKKLGLVS
                     GKKADLKMALELTRQLRQFDCNDPVKYDFALFGLGVEEKFWH"
     gene            complement(26066..27121)
                     /locus_tag="VGA21_01755"
     CDS             complement(26066..27121)
                     /locus_tag="VGA21_01755"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEX9649260.1"
                     /translation="MIRFFLTSIFLVSFTITGFCANLDSLRLEKEGEHYFIIHQVDEK
                     ETLYSLSRRYGVPIPKILEQNPEAKSGLGIGEILKIPYIPREKEENGEIHIVKTSETL
                     FFISKLYNVTVDDLIKWNNLNSNALNVGQKLIVTAGGSPQMPAHAGDSIFHIVKASET
                     LYAISRQYEVQVLEIRQWNNIVNNSISLGQRLFVGMRRDNNNVVAAPSNETEIIPEVS
                     DVIQVEKVEPPKAQPPIAKVEKEPEEVVYVGRTGPETVTNSSGYTRLVENGMGGIIEG
                     SEDNKKYLALHKTAKIGTIMQVRNEMNNKVVFVRVLGRLPDTGANEGMLIRLSKAAYD
                     RLGAIDKKFRVEISYVP"
     gene            27267..28640
                     /locus_tag="VGA21_01760"
     CDS             27267..28640
                     /locus_tag="VGA21_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020528429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEX9649261.1"
                     /translation="MKKFFIAPLAVFLFSLGMVSGQNDKQSTILVLDIKDAIDPRMSR
                     LVRLALEKATDTTADILLIEMDTYGGALTDADDIRTMLLEFKKPTWVFINRDAASAGA
                     LISIACDSIYMASGASIGAATVVTADGSAAPDKYQSYMKSIMRSTAEAKGRNPQIAEA
                     MVDQEIEIEGITEKGNVITFSTSEAIQNGFCEGQRTSIDEIIQKNGISNYNVVRYERS
                     TAEKIIAFFLNPFVSGILLLVIVGGIYFELQTPGVGFPILAAGIALLLYLTPYYLNGL
                     AENWEILMFFAGIGLLVLEIFVIPGFGIAGILGIIFAGGSLVLVMLNNDFFDFSFVEG
                     DKIVMAVTTTLGALIGSVIVMFIGGVRLTNTKIFKRISLQTVQDKESGYTSRFVTTLM
                     TGKQGTAFTILRPSGKVLIDGEVYDAFTRGEYIEKDAPVIVVDEEGTSLKVKAAPEIK
                     SSKKKKN"
     gene            28637..29383
                     /gene="kdsB"
                     /locus_tag="VGA21_01765"
     CDS             28637..29383
                     /gene="kdsB"
                     /locus_tag="VGA21_01765"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013455330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HEX9649262.1"
                     /translation="MNLKIAGIIPARYKSTRFPGKALAELKGKSMIQRVYEQSLKAKS
                     LDSVIVATDDQRIFDHVAGFGGMAVMTSPDCRNGTERCYEVIAGNERKFDFIINIQGD
                     EPFISPNQIDQVSSLFSLDIQMVTLAKKIDTMEELFNKNVNKVIFTKDHFAIYFSRTP
                     IPYLRNFEKDAWVENHDYYKHIGIYGYSARVLKEIVNLQPTPLEIAESLEQLRWIENG
                     YRIKMGITDIDSQGIDTPDDLERILNKINK"
     gene            29383..30162
                     /locus_tag="VGA21_01770"
     CDS             29383..30162
                     /locus_tag="VGA21_01770"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HEX9649263.1"
                     /translation="MDWEYLVIPLTALIASLLTFFSGFGLGTILTPVFAIFFPVELAI
                     ALTAIVHLLNNLFKFLLIGRNTVTRIFLKFGTASVIGAIVGSLLLITMRELSINYSVS
                     IAGFAIQTNLMKVVIGLLILFFALFELIPTLRNYQFPKSWFVPGGLLSGFFGGISGHQ
                     GALRSAFLIKYGLAKEQFIATGIAIALAVDIVRITFYGTSFYDSSILEMKGILSITVI
                     SAFLGASLGRQLLDKVTLKYVEQTVGGFLLIMGLLITLGIV"
     gene            30250..30933
                     /locus_tag="VGA21_01775"
     CDS             30250..30933
                     /locus_tag="VGA21_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010855487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b subunit"
                     /protein_id="HEX9649264.1"
                     /translation="MAWLKSTLSSSIGKKVLMALTGLFLILFLVIHLAGNLQLLINDN
                     GESFNIYAYTMGHNPLIRAVSILNFAFIFIHIIWSILLTRQNRLARGINYAVTASSAN
                     SSLASRNMGILGFIILIFLVVHIKTFWFEFKFGSIPTISYEGVEMHNYFRVVNEAYQS
                     FPYVAFYVVCMGFLAFHLSHGFQSAFQTLGLNHVKYTPFIKKVGAGFAIIIPVLFALI
                     PVMMYLKNL"
     gene            30944..32866
                     /locus_tag="VGA21_01780"
     CDS             30944..32866
                     /locus_tag="VGA21_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019600448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HEX9649265.1"
                     /translation="MNLDAQIPEGPLAEKWTKHKFNLKLINPANKRKYDIIVVGTGLA
                     GGSAAASLAELGYNVKAFCFQDSPRRAHSIAAQGGINAAKNYQNDGDSIFRLFYDTIK
                     GGDYRSREANVYRLAEVSVNIIDQCVAQGVPFAREYGGLLANRSFGGAQVSRTFYARG
                     QTGQQLLLGAYSALSRQISAGKVQMFPRTEMLDLVMVDGTSRGIVTRNLITGKIESHS
                     AHAVVLATGGYGNVFFLSTNAMGSNATAAWRAHKKGALFANPCFTQIHPTCIPVSGHY
                     QSKLTLMSESLRNDGRVWVPKKARPGFAARDAVNIPEAERDYYLETKYPSFGNLVPRD
                     VASRNAKFVCDEGRGVGSSGLAVFLDFADAIKRDGRNTIEAKYGNLFDMYKQITGDNP
                     YEMPMMIYPAVHYTMGGLWVDYNLMSSIPGLYALGECNFSDHGANRLGASALMQGLAD
                     GYFIIPYTIGDYLARTSYDKTPVSHDAFKEAENGVRESVTRLLNIKGNKTVDELHKEL
                     GKVMWDYCGMSRNEEGLRKAKGLVKEIRDDFWNNLKVGGSNEELNMSLEKAGRVADYL
                     ELGELMIDDALVRSESCGGHFREESQTEEGEAKRKDDEFAYVSAWKYGGKNVDESLVQ
                     EHLIFENVKLTQRSYK"
     gene            32870..33622
                     /locus_tag="VGA21_01785"
     CDS             32870..33622
                     /locus_tag="VGA21_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009185381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HEX9649266.1"
                     /translation="MKLTLRIWRQKDALSKGSFKSYSVDNVSGDMSFLEMLDVLNEDL
                     THKGEDPVHFDHDCREGICGMCGLVINGRPHGPKSAVTTCQLHMRSFKDGDTVTIEPW
                     RAKAFPVIKDLVVDRGAFDRIIQAGGYISVNTGGVPDANEIPIPKKVADEAFDSATCI
                     GCGACVAACKNASAMLFVSAKVSQLALLPQGKPEKRTRVEKMVAQMDLEGFGNCTNTG
                     ACSAECPKEISQENIARLNREYLSAKVSSDNL"
     gene            33646..34845
                     /locus_tag="VGA21_01790"
     CDS             33646..34845
                     /locus_tag="VGA21_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011584111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEX9649267.1"
                     /translation="MNNKVSEEQKSVIANRVNNLPESFIREILKVTTRPDIISFAGGL
                     PNPKLFPVELLQTVTNKVFVEEGREGLQYTVSEGFQPLRHKISERYSDKGLHVKPENI
                     LITNGSQQGLDLTCKLLLNPGDGVLVCNPTFIGALQSFSIYEPSYSSVAIYPDGPDVE
                     QIANVLREKEIKLFYCVPNFQNPTGATFSEEKRLQITRLIHETETIILEDDPYGEIKF
                     TSAKIPSFKQLLPEQTILLGSFSKIVAPGLRTGWLAAPDHLIRKLVILKQAADTHNNH
                     LVQRILFRFLTECDVEGHIARICEVYKFQRDLMMDCIKEHFPKEVKVFSPDGGMFLWA
                     ELPEHIDAYAVFEEAIKEKVAFVPGKTFYPNLDVNNTFRLNFSNSNEDKIRKGIESLG
                     IAIKKFL"
     gene            34846..35985
                     /locus_tag="VGA21_01795"
     CDS             34846..35985
                     /locus_tag="VGA21_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019598692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HEX9649268.1"
                     /translation="MGKIVITCNPRVSEILAREVESLGLTVDSIEPKGVITKGSLEDT
                     YYLNLNLRTGNKVLWEIGRFNARNADELYAEARKIEWDKYFPLQKMFSIESFVKNDTI
                     KDTRFANLRLKDAIADFFVSKTGKRPNSGADKSEVVLFLHWLDDEVALFINTSGETIA
                     KHGYRQAAVKAPMIESLASAVLMSTRWDMNSPLVNPMCGSGTLAIEAALLALNRCPGH
                     FRENFSFMHLQGYNPRVWKVIKSEARKVERTGLAFPIIASDNSEEAISVARENAQYAG
                     VSNLIKFQVCDFSNTLIPKQRGVIIMNPEYGERLGDVEQLEKTYEEMGDFLKQKCTGY
                     LGYIFTGNLDLAKKIGLQAQRKIEFFNGKIDCRLLEYELFAGKKQ"
     gene            36040..38313
                     /locus_tag="VGA21_01800"
     CDS             36040..38313
                     /locus_tag="VGA21_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009581588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX9649269.1"
                     /translation="MKTILQNIFYSFPVQLLLNHFRKNHVLMAIWVILFAIVNGNIGS
                     SLGIHYLFLDPEYLNSVGFRSFLIMGVVIAGFSVAFHITAYITDGHRFTFLGQLAKPF
                     SLFSINNSVIPIAFLIFYLIKVVSFQVDNEYGTTASLVRNLGGFMVGYISMVTLLYIY
                     FRNTNKDIFKYVVCKVDEKLKQNIKVTRRNVMEKLDVARKKQIRVDNYLGIRMRFRKV
                     PDYQGFYDKATILQVFDQNHLNLVLIELFIFVLLLIIGVFRDYPVMQIPAAASGILFL
                     SVFVMFAGAFSYWFRSWAVSAVILLGITLNFIVQENFLKKNYEAFGLNYDLPEKEYNL
                     KSLSQTNSPGNILEDWSQTKEILENWRGKFPEINKPTMVFICASGGGQRASLWTMKAL
                     QAADSATNGQLMENTVLITGASGGLIGAGYFRELYLRKKKGDPVNLQNHSYLQDISND
                     NLNPIIFSLLVNDLIVSFQKFSYANMQYPKDRGYSFEEQLNKNTRYFLDKPLSTYKEP
                     ELKSEIPMMILAPTIMNDGRKLFISPQNVSYLNIGLIRQNPGFDTEIEGLDFHRFFED
                     YGSNDLRFLSALRMSATFPYITPSVTLPSNPPIEIMDSGVTDNFGISDALRFIYAYKD
                     WIARNTRGVLILAIRDSQKQNEILSTQNLTLIQKFSNPISSIYNNFENLQDFNNDNKI
                     IFANSWFQGEIQKIDLQYIPRINSSNKLERASLNWRLTSLEKNNIIDAINSPSNQKAL
                     ELLKRSLSPNPLIANED"
     gene            38445..41816
                     /locus_tag="VGA21_01805"
     CDS             38445..41816
                     /locus_tag="VGA21_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019946603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEX9649270.1"
                     /translation="MPVAVAPYKPKNHIRIVTAASLFDGHDAAINIMRRIIQSTGVEV
                     IHLGHNRSVHEIVNTAIQEDAQAIAITSYQGGHVEYFKYMYDLLKEKGATNIKIFGGG
                     GGTILPEEIKELQDYGIARIYSPDDGRSMGLQGMINDLVKNSDYTIGENVNGEANEIL
                     KKDSQAIARLISGVENFPEKSKIFLDTVVSQIQEKIPPVVGITGTGGSGKSSLVDELV
                     RRFLMDFPNKTIGIISVDPSKRKTGGALLGDRIRMNSINDSRVYMRSLATRQSNLALS
                     KYVKEAVNILKAARFDLVILETSGIGQSDTEIIEHSDLSLYVMTPEYGAATQLEKIDM
                     LDFADIIAINKFDKRGAFDALRDVRKQYKRNNQLWESKDEELPVYGTIASQFNDPGTN
                     NLYEALIKLIIDKTSADLKSSFEATNEMSEKIFIIPPDRVRYLSEISEANRNYTKWVE
                     EQYVIAQKAYNVHGTIELFKSADNEVVRRLKSEFDKLKLELDPKNFKLIEDWPAKKER
                     YSADEYIYKVRGKDIKLTTYTESLSHTRIPRVSLPKFEAWGDILRWLLNENVPGEFPF
                     TAGVYPFKRQGEDPTRMFAGEGGPERTNRRFHYLSFGQPANRLSTAFDSVTLYGEDPD
                     YRPDIYGKIGNSGVSICCLNDAKKLYSGFNLIDPKTSVSMTINGPAATITAFFMNAAI
                     DQQCELYIRENGLEKEVETKIREIFKGKENLRPSYQGKLPEGNDGLGLMLLGVTGDRV
                     LPSEVYQKIKARTLSSVRGTVQADILKEDQAQNTCIFSTEFSLKLMGDVQEYFIQNTI
                     RNFYSVSISGYHIAEAGANPITQLALTLSNGFTFVEYYISRGMDVDSFAPNLSFFFSN
                     GIDPEYSVIGRVARRIWSKAMKLKYNANERSQMLKYHIQTSGRSLHAQEIDFNDIRTT
                     LQALYAIYDNCNSLHTNAYDEAITTPTEESVRRAMAIQLIINKELGLAKNENPLQGSF
                     IIEELTDLVEQAVLYEFDKITERGGVLGAMETMYQRSKIQEESLHYETLKHSGDYPII
                     GVNTFLSSKGSPTILPREVIRATQEEKENQIATVRSLHHQNEMLTKENLNNLKLAAIQ
                     NKNVFGILMEASKYCSLGQITHALFEVGGQYRRSM"
     gene            complement(41820..43772)
                     /locus_tag="VGA21_01810"
     CDS             complement(41820..43772)
                     /locus_tag="VGA21_01810"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9649271.1"
                     /translation="MQEFIKSILEIISLLVGIGERAQLGGRANGRIRLVNRISLIFAV
                     TSFIQSPVFFLLTDSLFIFSASCLIGLGFLIPLFINWKKRYTLAKICLISYICLANIF
                     FTQSLGNERLGQMLCFALITLPPMFFETEKTIWKYLCYLFPVITFLSLEFLDIMPEIE
                     VQSNFFSILQWILITTIFSLSILSVNGFSQNVNSLLGKLEILLQDSQTKNKKLEESQF
                     LLKESKDEMVVANRELIKYKNNLERMVEERTKALDLSIRHLELAQDEMRLAKDQAVKA
                     NMAKTEFLANMSHEIRSPLNAILGFTQLLNMLARQKHPDLETLEILDKIHISGENLSE
                     LINNILDLSKIEARQMILNEEDLHLKQLVKGIYHINKGKAGEKNINFQYFYDDNIPDV
                     VLLDRTKTNQIMMNLVTNAIKFTDPFGKVFLKVQADNNSISIKVEDTGTGVPGHMHES
                     IFHPFVQADGTITRKYGGTGLGLSITRKMVHMMSGSITLESEPGKGSIFEVKLPLRAG
                     TQRIELTEKEFTSSFIKPGVTVLLVEDNSMNQEVMKRFLDLNGFRVIIAENGEEGIIA
                     FKKYNPEIVLMDIHMPVMDGLQATKEIRNSGNLSVPIIAISADAFNEQKENALAMGCN
                     HYLTKPIDFTHLQEILIKFLPEYSTK"
     gene            43864..44271
                     /locus_tag="VGA21_01815"
     CDS             43864..44271
                     /locus_tag="VGA21_01815"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEX9649272.1"
                     /translation="MVITTRYLEDDLFEASNETGASSRIDMRNKEVKKNLSPVEGLLG
                     SLGTCIGGDVIDILRKRRKTVIGLTLVTNGTRRDHHPRAITAIRCKVVLESPDATVEE
                     LAKAAQLVVDKYCSVASSLKPSVNLSCEIIRPA"
     gene            complement(44258..45130)
                     /locus_tag="VGA21_01820"
     CDS             complement(44258..45130)
                     /locus_tag="VGA21_01820"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019943295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX9649273.1"
                     /translation="MKIVSPVWGDYELIDSGGYEKLERFGKYVISRPEPQAIWSKGLS
                     DPDWHQQADAKFNKLKGSADKGQWSLKKGMPEQWWVQYHLKNSKLNLRLGLTSFKHVG
                     IFPEQADNWNFIYDTIKRMNSKTTILNLFAYTGAASLAARAAGAEVVHLDSVKQVVTW
                     ARENMEQSKLSGISWVIEDALKFVNREVKRGRKYQGIILDPPAYGRGPKGEKWLLEDL
                     LPELLILCEKLLDGRNRFLVLNLYSMGLSPLVAESLVNSIFKLKSDAGELFFQDRAGR
                     KLPLGVFSRFTTQA"
     gene            45184..45984
                     /locus_tag="VGA21_01825"
     CDS             45184..45984
                     /locus_tag="VGA21_01825"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEX9649274.1"
                     /translation="MSEYISSTKNPKIRNLIHLKKSGVRKKQSLIIIEGEKEVKMALE
                     AGVKIPTLFICREFLKIELDQLFSLGIKTSDILNISSEVFNHVAYRQNSGGIIGIGIP
                     NNLQLSSLNPGKNSIYIILEAVEKPGNLGGILRTADAAGVSGLIVCDPKTDIYNPNVI
                     RSSLGCIFSVNTVTASSEAALAWLRQNQVISFATHLATEELYFEQDFSGRIAFVMGTE
                     SEGLTDFWKTGADKLIKIPMLGQADSMNVSVATAIVLFEALRQRDFVV"
     gene            46154..46483
                     /locus_tag="VGA21_01830"
     CDS             46154..46483
                     /locus_tag="VGA21_01830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649275.1"
                     /translation="MRAKYIFGKISFLIICGLFVSFSLVKEFSDLESSTNGKAEPIQT
                     LKTSEDSSHENIVNLKFYDCNEKLIYKTQVDLNDASRKKDKRLMKNLKASELIMKVDH
                     TLFFRIN"
     gene            46568..47401
                     /locus_tag="VGA21_01835"
     CDS             46568..47401
                     /locus_tag="VGA21_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019990269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005216 - monoatomic ion channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HEX9649276.1"
                     /translation="MPGNERPEKKPSLKELWYTIIFEAETPAGKRFDIILLVAILLSV
                     MAVILESVSTISIDFGEELRIIEWVFTVIFTMEYLARLYVIKNKIKYAISFFGIVDLM
                     SIIPTYLSLILAGTQTLLVIRSLRLLRVFRIMKLVRYLEEAGNLSNALKASKAKILVF
                     LGTVFILVILVGTLMYMVEGPRNGFTSIPTSIYWAVVTLTTVGYGDIAPQTVLGQSLA
                     AVVMILGYGIIAVPTGIVSAEMVRQQRTIEKLPTQVCGNCMEEGHDIDAIHCKHCGAK
                     L"
     gene            47408..47908
                     /locus_tag="VGA21_01840"
     CDS             47408..47908
                     /locus_tag="VGA21_01840"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009583564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX9649277.1"
                     /translation="MIPLEIKALDKSHWPAVSKIYADGLETKIASFETTCPSWEDWDM
                     NHHKFCRFVICEDKNAIGWIALVPVSNRKVYAGVAEVSIYISKESQGKGVGKMLMSHL
                     LNEVEKHGIYTLQCSLFPENRVSYALHSAFGFREIGYREKVAKLENEWRNTILMERRS
                     KSEEFN"
BASE COUNT        14428 a         9454 c         9660 g        14438 t
ORIGIN      
        1 gtagctggtg tgtccaaact gaggatccca atcaattcgt atatggttat tgtaaggtgt
       61 cagggtcacg ttcagcatct cctcgcaggc atcgccccac cggaaggtgc gtacctcgct
      121 gtagccatcg ttcttgtagc ggtcataccc ttcggtgttg gcggctttta cccgccaggc
      181 atactgacgg cccggaacca gcatcggatc gctcagggta tagaaatacg ccgtgttgaa
      241 ggtggtggtt tcaaagtacg gctgggtaga tgccgcaatg ttgtagggat tgctgccctc
      301 gggcagtatc tcatagagtt gcagggtgta ttcggttagt cccatgttgg gggatttctg
      361 ccggggactc cagctgaact ggatgttctg cgggtcccgg gccactagtt tttcttcttt
      421 catggggtag ttcagaaacg ggggatcggc caggatgatc caggcctggg ccatggccga
      481 attactcact accgaggagc ggtagtagtc cagcacctgg atggtgaacc ggtaaaagcc
      541 ttccggcagc atgcccagtc gctggtaatc ccgcttgctg atgcccagta agtccaggtt
      601 ttccaaactg aaatagggct gtagatcttc ggcggttagt atataaggtg tgccgccact
      661 gagtggaatg ggctggggta gaaaggtgga tttggttttt atcgtgatgc ccactccttc
      721 cactatcagc ctgagcttca cggggtactg attcttggtt aaatcagtca gcagtagttg
      781 cacctgcagc agattactgc ccggcatgcc atactcataa agtgccaggg aatagggcgg
      841 attcaggaag gccgatacct gcacgggggt ttgttgtgcc attagagcca atggagccac
      901 aaacacataa taaaatatta gaaaaagtct atttatcaca aaaaaaataa gaaatacttt
      961 gataaatatc aaaataaatc taaatagtgc aatcaaaaga tgaaaatatt tcttgaatta
     1021 gtaataattt ttgggggaat aacaggaaat gtgcatccct gccaggcttg tatttaagaa
     1081 ctcacggaaa aatcactgga agatggtttg atcaggttga attagaagcg ggctgccatt
     1141 atcaaattca aatccttatg tttcattttg aatttcttgc ccagaatttc attggtcagg
     1201 ctgccattat aaatatatac gcctttcata aaccacttat gcgtaaagat catatcttca
     1261 attcctccat cctccgcagc ccgcaaaatt gtgggtgtga aaatgttgga taacgcggtt
     1321 gtagcggttc gtgcaaccct gcttggaatg ttgggaacac aataatgaat tacatcatat
     1381 tttctgaaga caggattctt atgcgttgta attcgggaag tttccacgca gcctccctga
     1441 tcaatgctgc aatcaataat tactgagttt ggcttcatat tcattaccat ttcttcagta
     1501 atcaaaattc tcctttgatt tttatccagt ctcacagcgc ctataaccac atccgcattc
     1561 ttcagactgt cactaagcgt cttggtatca actgtactgg tgtaaacctg ctgaccaagc
     1621 gcgtgtcgga ttcgtcttaa cttatgaata tggttatcaa aaatctggat gtctgcaccc
     1681 aggccaagcg cggttctcgc cgcgtattcg gcaaccgttc cggcaccgac gataacaacc
     1741 tgggtaggcg gaaccccggt aatgccaccg agtattactc ccctgccatc atttacactg
     1801 gaaagatatt ctgcggctat aagcattact gtactcccgg caatttcact catggctctt
     1861 acaatcggaa agccaccaac cttgtcttca ataaattcaa atgcaatggc cgttatcctt
     1921 tttgacatca gcgtttttat gtactctgca tcctggtttc ccagctgaag ggcacttaac
     1981 aaaactttgc ctggtcccag gtagtccatc tctttggcag taggcggctc aatcttcaat
     2041 aaaatctgtg catcatacgc ttctttcgga gtattcacta tctctgctcc cgcttcactg
     2101 tattcatggt ccgtaaattt tgactttagt ccggctcctc tctccaccct gatcctatgg
     2161 ccgttgttag ttagtaattc tacggcatcc ggtgttagtg aaacccggtt ttcctgaaag
     2221 gaaatttctt tgggtatgcc tatataaaaa gaatttttcc ctgattttac tttttcaagt
     2281 tgttcctgag gatataacga tcccttaacc aattcttcta atcctgattt tttcattcta
     2341 gtcattcctg gtaatctgaa tttttcttaa gtctgactcc ttcgacataa caatttttag
     2401 ataaacctca ggaagcaagt taggtatctg atccggccat tcaatcaaac ataaattgcc
     2461 cgaataaaag tattcctcat acccgatatc cattgcttca atttcatttt ctattctgta
     2521 aaaatcaaaa tgattgatct tttttgaatc tgaaatgaaa tactcattta caatattgaa
     2581 agtaggactc gtaacggagc ccttaacact caggctactg caaatttcct taatcagggt
     2641 tgttttacca attcccatct caccgtaaaa taaccagact tttatgtttt cagcaaaatc
     2701 aatgatttcc ttcgaaatac ttggtaactc gttgatgctg tgatacttaa tttctaattc
     2761 tccggctgct ttttcaacca tttttcgaat ttaatgaaat aatcggaata atcatttctt
     2821 ccaatgaaac accgccatgc tgaaaggtat ccttataata gttaacgtag taattgtagt
     2881 tattcggata ggcaaagaaa tgatcctcaa cggcgaaaac ataggaagta gacacattta
     2941 ttttaggcaa atgaaaagct tctggtttct tacaaaccag aatatctctt ccatcatacc
     3001 caaggttttt cccttgctta tacctgaggt tagtgttagt atttttatca ccgataatct
     3061 taaatggttt ttttaccctg atagttccgt ggtcagtcgt tattattaag cgggcattca
     3121 tttccgcaat cttcttcaaa gattcgagca atggagaatg gagaaaccag gacaaagtta
     3181 aggagcgata agcggattca tcgggtgcca attcgcgaat catggccatg tcagttcttg
     3241 catgtgaaag catatcgaca aaattgtaca ctatagcgtt aagctgattg gctctcagat
     3301 tgggaatggt atcaacaagc tgttttccct gattaacatg ttttattttg acataagagg
     3361 cttttaggtc caggttgttt cttgcaagct gcttttctaa aaattcactc tcaaaattat
     3421 ttttgccatc ttcaacatct tctccaaccc aaaactgagg ataagtattt gccatttcca
     3481 acggcaacat cccggagaaa atcgcgttcc ttgcgtaggc agttgtagtt ggcagaatgc
     3541 tatagtaata atcctcttct tctattgtga agtattcctg aattacaggc tccagtattt
     3601 tccattgatc ataacgcaaa ttatcaatta caagaaaaaa gattggccgg tctttatcca
     3661 ggtagggaaa tacctttttc ttcattaatt gatgagacag caacggttta tcacttgccg
     3721 gatcgtttag ccaactctca taattctgac ttacaaatct gacaaagttt gaattagctt
     3781 cggttttttg catgtcaaaa acttcagcca tatcccggtt gtcagaactg ttgatggcaa
     3841 gttcataaaa aacgagtttc ttatagatgt cgatccactc ctcatggctt agttcgctat
     3901 taaaagcact tgctatactg cgaaattcct gttgataact gatgttggtc cgttctgtaa
     3961 tcagtcgttt gttgtccagg attttcttaa cggacaaaag aatctgattc ggattaagag
     4021 gctttataag gtaatctgca attttcgctc cgattgcctc ctccataata tgctcttctt
     4081 cactcttggt tatcataact actggaagat tgggcttact gctttttata tgattcaacg
     4141 cctgcaaacc actcataccc ggcatatttt catccaggaa aacaacatca aaattttgct
     4201 tttcacacat gtcaatagca tcagcaccac tatttacagg agttacatta tatccctttt
     4261 tgtcaaggaa caggatgtgt gccttaagta agtctatctc atcatctgcc cacaaaatat
     4321 tatatctttg catatatgcc aattacaact tcgaagatac ttaatatttt ttacttcaaa
     4381 tcgtctaaaa attcttccga aaactttgaa taagaaaaaa attattaatg accccgtgtt
     4441 gggtttcatt accattaatt ctgacctgat actaaagtta attgatcatc cttatttaca
     4501 aagattgcgg cggataaaac aacttggcct gacagaattt gtttatccaa gcgcggtgca
     4561 tacccgcttc catcatgcac ttggagcgat gcatttaatg gagctcgcgc ttaacagtct
     4621 gcgatcaaaa ggaatcaaaa taactgatga tgaatatgag gccgcgctga ccgccatttt
     4681 gcttcatgat atcggccatg gcccattgag tcacaccctt gaatattcac tgacggaaag
     4741 agttgagcat gagatgatta ccgttctgat aatgcaaaaa cttgatcagg aatttggtgg
     4801 aaggctgaag ctgtcgatcc gaattttcaa aggtgattat gaaagaaact tttttcacca
     4861 gttagtagca ggtcattttg atatcgacag aatggattac ctgaagcggg acagctttta
     4921 taccggagtg tccgaaggta atgttggagt aagtagaata atccacatgt tgaatgttgt
     4981 tgacaatgaa cttgtggtag acgaaaaagg aatttataat attgaaaatt tccttatagc
     5041 caggagattc atgtattgcc aggtatactt acataagacc acagtagctg ctgaaaagat
     5101 gatggaaaat ctctttgcca gagcgaggtt tctattaaaa agtaattcag ctcttgttgt
     5161 tcctccggca ttaaaaccat tccttgtgaa aaggatttct gcaaacgatt tccagaaaga
     5221 taatttggta cttgagaatt ttttgaatct tgatgactct gacatttggg aagctttgaa
     5281 aaattggaaa tcctcatctg ataaaatatt atcagatcta tcaaactgga ttttgaaccg
     5341 gaggcttttc accattgaga tgtcaggagc tccttttgat gcggcgctga ttgataatgc
     5401 acagaaaaaa atatctgaag tgtttgaaat agataatcct gctactcaat actatttctc
     5461 acacggcctg ctttttaatt cagcatatgg tggtcattat ggtccgatta aggtattaac
     5521 taaaaaaggt ttgatactac tcgaagatct gtcagaggta ccggccattt cagaattaag
     5581 caaaattgtg cgaaaatatt acctatgcta ccctaaaaat gtatctttgt gagccttgag
     5641 aaatgatttt ttattgaatt aaccttatgg agtttagtat tgaagaaatc gccgtaattg
     5701 ttgggggaga gatagaaggc aatggcagtg atactattaa taaactgagc cggattcagg
     5761 atgctaaaga gggaagcatt tcttttttag caaatctaaa atacgagcct ttcatatata
     5821 aaacagaagc cacagcaatc attgtaggaa cagagtttaa ggctagaaag aaacttaatg
     5881 taacacttat cagggttgaa gatccgtatg ttggatttac tcttctactc gaagaatatc
     5941 acagattgat tagtctgcag cgcgagggag tagaggaacc aagttacctg ggtaataaca
     6001 caacaatagg tgaaaagatt tatcggggag cgttttccta cattggctcc aatgtgaaga
     6061 tcggcgataa cgtaaaaatt tatcctcagg catacattgg ggataatacg actgtaggca
     6121 ataatactat tattcatagc ggagttaaaa tctacccggg aaccaaaatc ggaactgatg
     6181 ttgtattgca cgccggagcc gttatcggaa gtgatggttt tggatttgca cctcaggatg
     6241 acggttcata caagacaatt ccccagatgg gtaatgtaat aattcacgat aaagtaagta
     6301 ttggagccaa cactactatc gattgcgcca cactcattaa tgattcaacc gaaatcggag
     6361 agggaacgaa gttggataat ttaattatga ttgctcataa tgttcatata ggtaagaata
     6421 cagttatagc agcccagtca ggcgtttcgg gttctaccag ggttggagac aattgtgtag
     6481 ttgcaggcca ggcaggaatt attgggcatc tcgagattgc caacaatact acgatagccg
     6541 cgcaatcagg agttggaaaa agtataaaag aacccggcca ggttttgatg ggaagcccgg
     6601 gcttcgacca taaacagtat ttgagagtct atactgtctt caaaaaatta cccgatttgg
     6661 aaagcagaat taaagaactt gagcaaaaag tactaaattt gccgaccatt taggggtttc
     6721 atgtatacca atcagcacac tatcaggaaa tcagtaactc tttccggtgt aggattacac
     6781 acaggagttc aagctaacat gacttttgtt ccagcaccgg tgaaccacgg aataaagttt
     6841 cagcgcgttg acctggaggg tcagccgatt gtagaagcgg atgttgataa tgtggttgat
     6901 ttatcaagag gcacaactat tgaaaaaaac ggggcgcgtg taaatactgt ggaacatacg
     6961 ctggctgcat tagttgggct ccaaattgat aatatcctga ttcaactgga tggccctgaa
     7021 cctccgatta tggatggaag ttcaaaaccc tttgtagaaa ttctccggga tgccggatgt
     7081 attgatcaaa aaactccacg gaactttttt gaagtgcagg aaagtatctt ttatcgtgat
     7141 ggatcaaggg atgtggaaat tgcagcactt cctctggacg attatcgact aacagtaatg
     7201 atcgattata aatctccggt tctgggaagt cagcatgctt cgctaaatga tatatcccaa
     7261 tttgacaagg aaatagccag ttgcagaact ttttgtttcc ttcacgaatt ggaaatgctg
     7321 cataagaata acctcatcaa aggcggcgat ctaaataatg ccattgtagt tgttgacagg
     7381 gtagttgaag accatgaact tgaaagtctg gcaaaactct tcaacaagcc caaagtggaa
     7441 gtaaaaaaag aaggcatctt aaataatgtt gagctccggt ataaaaatga acccgcgaga
     7501 cacaaactat tggatgtgat tggagatctt gccctggccg gaagaccgat aaaagcacaa
     7561 attcttgccg caagaccggg ccacgcggca aatgttgcat ttgcccgaaa gctgaagaaa
     7621 gctatgcagg cagcaaagtc tcatgaagtt ccaaaatttg accccaattc tccggcagtt
     7681 gtcgacatta ataatatcct taccactctg ccacacaggt ttccattttt aatggttgat
     7741 aagattttcc acctggatga aaaaagtgta gccgggataa aaaatgttac cataaatgaa
     7801 ccctatttcc aggggcattt tcctggaaat ccagtaatgc caggggtgct ccaggttgaa
     7861 gccatggtac aggtgggcgg tatcctggtt ctgaatactg tgagtgaccc ggaaaattac
     7921 tggacttatt ttctggctat agagaatttt agatttagaa aaatgattag acctggcgat
     7981 tctctgatta ttaaatgcga attattatct cccatcaaaa gaggtattgc aaaaatggaa
     8041 ggcagagcat acgtcgggaa tactcttgtg tgtgatggag tgatgacagc aagtattgtt
     8101 aggaaagatt catgaaccag cccttagctt acatacatcc ttctgctaaa atcgcccgga
     8161 atgttgtaat tgaaccattt actgtaattc atcaaaatgt tgaaattgat gaaggtacct
     8221 ggattgggtc aaacgttaca atttttgaag gagcaagaat tggaaaaaat tgtaagattt
     8281 atcccggggc tgtcatctca gcgattccac aagatttgaa gtttggcggt gaagatacca
     8341 ccaccgaaat cggtgacaac actgtgatca gagaattcgt tacaatcagc cgtggtacgg
     8401 ttgatagata caaaacagta attggagcca ataatttgct tatggcttat gtgcacgtgg
     8461 ctcacgactg tcaaatcgga aacaattgca ttttagccaa cgcagtgcaa ttggcaggcc
     8521 atgttcaaat tgatgatcac gctattattg gtggggcaag tgctgttcac cagtttgtta
     8581 aaatcggagc tcatgtaatg atttccggtg gctcattggt aagaaaagat gtgccgcctt
     8641 atactaaagc cgcccgtgaa ccactaacct attgcggcat taattccata ggactcagga
     8701 gaagaggatt tgaatctgaa aaaattaccg agatccagga aatataccgc tatatattcc
     8761 tcaaaggcca caacaattct gatgcgcttg attttgttga aatcgatctg cccccatcaa
     8821 aagaaaggga tgaaattatc cgcttcatca ggaattccga caggggtgta atgaaaggtt
     8881 atagtcacta atgaatattg aagtttccaa cctgggaaag tcattcaacc accatcggat
     8941 ttttcataat cttacctata cctttcagca agggcaatct tattcattat taggccccaa
     9001 tggcagtggc aaatcaacgc tgctaaagat tctttcggga ttcatgctgg catccaacgg
     9061 catcattact tataagagcc gtgataaaat tattgatccg gaatcatggt ataaatatca
     9121 atttattgcc gcaccataca tggagcttcc tgaggaactt actttgagcg aatttctaag
     9181 atttcacttt aaatttaaga agttcagccc tggatatgag tttgataatg caataagtaa
     9241 atgtggattg cagggaaagg atgaggtgca aatcaaaaat ttttcttcgg gcatgaagca
     9301 gcgattaaag ctggcccttg gaatgtattc aagccaggaa atattattga tggacgagcc
     9361 ggctactaat ctggaccaat ccggagttga atggtatctt agggaaatca agggcatatt
     9421 acctgaaaag ttggttatta ttgcttcaaa tactttgatt gagcatgatt tttgccaaaa
     9481 ttgccttaat ttgaatgaac ttaagtaggt tacatttagt gatttgctgt tataaatgaa
     9541 tgattattta gacagttgat tattgaattt tactgagaga aattattaat ttgcttaaca
     9601 tttaacaaac cctataaatg aagatagtaa gaaaagcttc cattctgctc atggttttgg
     9661 cagtattgct tgtttttggc tgcaagaaga aaaaagatat tgaacccgat ccggaagctg
     9721 aagcaattgc taaattagtt tctggcgcat gggttcctac tgccgtcacc cttgatagcg
     9781 aaaataggac agctgacttc actaacttta gtttaaccat aaccgaaaac aaaacttact
     9841 ccactttagg ggttgaccca atttacagca gtgtctggcc atcatccggc agctgggatt
     9901 ttaagagttc gggttcggca attcctgatt tgacaactat tatcagggac ggaagtcttg
     9961 aaatctctat tgatgtgttg tcagagactc agtttgtgat gtcttttact tttgttaccg
    10021 gttctgttga agggacttcc agtacagaag gaaattatat tttcagcatg gaacacaact
    10081 aattgtatta atttttcaaa agatataatc gaaggctctt ttgcggagcc ttttttcttt
    10141 taaaaggaaa tgaggtttga tcgaagcaat gacccaactc tttatttcag atagattaca
    10201 gttactccat cacctccccg ttccaaatgt tcattttcaa atttctgaat atgactcagt
    10261 cttttaatat gctcccggat aacttttctt aaaataccat tcccttttcc atgaatcacc
    10321 gatacctggt ttataccccc taccagagca ttgtccaaaa atttatccac ctggaaaata
    10381 acttcttctg ccctcagccc ccttacatca agagacgttg aaagttgttg aactttcctt
    10441 tcaaattgat accctgatgc cggcctgggg gtcaaaggtt tcgcatcagt ttttcgcaaa
    10501 tcttttagct ttacttttgt tttaatatta cctaccaaaa ccagagcttc tttccctctt
    10561 atctcaagaa tttctccaac cataccttcc ttattgattt ctacagtatc atttaaacag
    10621 aaaaaatctt ttcccggaaa ttcgcctgat ttttccggct caactttcac tttgtccctg
    10681 aattcatcta attgatttcg aattttacgg gtttcttttt tctcagcttt attttcttta
    10741 atgtgccgga tggttttttc tatttcccgg ttagcctcat tcagcaaagt ctcagccttc
    10801 atggatgcat cattaattaa ttctctctgt tccccttcga tttccttctt aagctgcaaa
    10861 tacatttcct tgtttaacgc tgcttcccgt tctgatttat ccaattgcaa tagcctatta
    10921 gaaagctctt tcctttcctg atccagcctg gctaaaatgt cttctaatga aaccgcctca
    10981 gaaccggcca attcctttgc cctggcaata atctcctccg gcaggcctgt cttttgagca
    11041 acctccaggg caaaggagcc cccttgtctt ccttgttcaa gtatataaaa aggtcgcaga
    11101 ttttgtttgt cgaagagcat agccgcattc gaaaggtatt ctgacttttc agcgaagtgc
    11161 ttaagattag agtagtgagt ggttattagt ccccagcatt ttctttggat aataccttcc
    11221 aaaattgctt ctgcaattgc accgccgatt tgaggatctg tacctgaacc aaattcatca
    11281 atcaggataa ggcttttttc atcggcattg gccaggatct cgctcatatt ttttaaatga
    11341 gatgaatagg ttgaaaggtc attctccaaa gactgctgat cgccgatgtc tagaaaaatt
    11401 ttattaaata ttttgaatga agaagtttct tccgcgggaa ctaatagccc gcattgaagc
    11461 atatactgga ataagcccac agtttttaac gcgactgatt taccgcctgc attcggcccg
    11521 cttatcatta ctattcgctc ctttttatcg attaccaaat tcagcgggac agcttctttg
    11581 ttttgcagga gcaacaaggg atgccgccca ttcactattt tcatcggttc atgcaaatca
    11641 actgcaggct taaccgcccc aatctttttg gcgaacaatg cttttgagcg ggtaaaatca
    11701 atggttgcca gaaattgcac agcctttaaa agaaatcctg cttctgctct aatctcatca
    11761 gagattttaa gcaggatttt aattatttct atcttctcct ggtgctgaag gttctgcacc
    11821 tggttagtga tttccagaat tgaatccggc tcaataaata ctatagatcc ggtggctgaa
    11881 taatcatgaa ttattccccg tatttttcgc ttgtgttcgg aagaaatggg tattactact
    11941 cgtccacctc tcaccgacac ggacatctcc ttcggaacaa atccatttaa gcttgcttcc
    12001 tggtatatcc ggtttatcac tttaccgagg gagccttcta cttctttctt ccttttcctg
    12061 attttattta gttctggtga agcattgtct ctaagttttc cggtctcatc aaattttaat
    12121 tgaattgctt ttataagagt tcgcggataa ttttccaatc gaagagaatt aagagttggc
    12181 caatcttctt tggataccat caggaatttg tcaatctcat cacaagtttt taagaacaga
    12241 taaatttccc agacttcatc ctcttctaaa aatgtaccct ggggtttcag ttttccgatt
    12301 gaaactcgtg ggtcatagta atttgaaaca ggaaaggccg ctggcccctg aagaatctca
    12361 agaaattcct catccaggcc caggctacat actaccaggg ctggatcggt ttcccatttc
    12421 atttggttcg tcaactcacg gccgggttcg aaaaggcaga actctttgag aagttccctt
    12481 atcccggaaa atcctaaact ttgttcgata tcattaattg cgctggtcac taccttcggt
    12541 ttccaaattt cctttgctca ttctttcata caaactcaag gagtctaata cggctgcata
    12601 tacttcctcc attaataaag ggtgggcgta ataaaattca aggcttcgat aatataatga
    12661 atcattcact ttatgttttt gcagcagttc atcttcaaag tattgaaata caacccttgc
    12721 agagtctttt gagatccgta actgccccgt ttgagcctcc agaatgtgta catcaatcac
    12781 gaaacttatc atctgatttt ttgacattac atcttttgga atttccactt cagaaacgca
    12841 tcctgtaaaa acacatacta tcgctagata aaatttcatt ttcatccgtt caaaatacta
    12901 ttaattgtca tatttttaaa gcccaaagtt atggagaatg aaggagttag taaaaaagct
    12961 tcgcaaatat gagattcaga tcagaaaggt ggttgattcc cagcggcaag gggactttca
    13021 ttctatattt aaaggttccg gattagagtt tgatgatgtc agagattacc aatatggtga
    13081 tgaaataaga actatcgatt ggaacgtaac ggctaaaggt catggcactt ttgtgaaaac
    13141 ctttaaggag gagaaagagc agacggtttt ttttgttttg gatgtaagcg gatcacagga
    13201 aataggaagt ccaggtcacc aaaagatgga tattggaaaa gagatttgcg gtgtcttatc
    13261 gctagcggcc atcaaagaac acagccaggt agggttactg tgcttttcga accaaaaaga
    13321 agtgtatatc aggccttcca aaggatcccg gcaagcgtat gcaattattt cttcattata
    13381 ccatttgaaa cccgaatcag ggaaaacgga tattaacaaa gcgatagttt ttacgttgaa
    13441 taccatcaaa agaaaaagtg ttgtgatatt tatctcagat tttattgaca aaaattacgc
    13501 caacaatctg aaagctctgg ctaagaagca tgacctggtc atcattcaaa ttggcgacaa
    13561 acgggaatcc gaactcccga aacttggtat tgtgccggtt tacgaaaaag aatcaaagac
    13621 caccacctgg gtaaatactt cctcacattt cttcagatca aatcttcagg atacgtattt
    13681 gcaaaacacg gaaaatctga ttcgattttg ccggaagaat caggctaact atttatacat
    13741 agatacgcaa gaagactttg ttcctaaact aatcagattg tttaaaataa gaaataaaac
    13801 aaggaaaagt gcgagataga attggagtgt tgatgcttct tattttcatt tctacaggtg
    13861 catccgcctg gcaagtgaca ccgaaaggta aatttctttc cgacagcact cgcattgggg
    13921 tagaactatt atatagttta tcggtgactt atccgctgga aagtgatatt gtcttccctg
    13981 attcattata tgatttctcg cctttcgagc ttaaccagaa agattacttt tttacaaaaa
    14041 gcgataactt tttcagtttt gatagtgcag tttacaatct ctccacgttt gaaatagata
    14101 cttttcagat gttggccctg ccaatttggg taatcggcaa cggggatagt attgcctatt
    14161 ttccagcacc ggatacagta atcttaaatc acattgtaac tcaaatgccg gattctttgg
    14221 cattaattga taatacaata tataaggaag ttaactatgc ttttaattac ccgtactttc
    14281 tgataagcat cggaattgcc tccattgtta tttttgtgat aatttatttc tatggaaagt
    14341 caatcagaag ggcttattat ttgtataaga tgaaacggtc acatgagagg tttgttaagg
    14401 catttaacac tacgattcat tccaataaac acaattggga aaaatcattg cttgaatgga
    14461 agaagtatat ggagcagctg gatgaaggtc catataccag gctcacaacc agggaaattg
    14521 cccggaaaac aaaagacaaa ttgttgctga atgcgctgaa agggattgat agggttatat
    14581 atgggaatta cgagcgggat accggcaaac agtattttga agacctgatt cattttgcca
    14641 ggaatgccta cgcaaatagg gtaaagcaaa taaaatatgg aacaggaagt tgaaaatatt
    14701 gtatggtact ctgctgaatg gttcagcccc ggtattttcc aaaactactc ctgggaaaac
    14761 caaattgcac tctatctctt atttctgatt ccaatcataa tcattgtgag atggctggta
    14821 aggaggaaat ttagtcagaa acttccggtt tctatcccga aagaaaatat taagtggaat
    14881 ccaataaccc tgctccggtt tatcccgaac attctgttag catttatttt caccctatta
    14941 ataattgccc tggcacgacc acaaaaaacc aatgaaaagg tggagcagtg gacagaggga
    15001 atagacatta tgattgtagt agacattagc gaatctatgc aaattgaaga ttttcaacca
    15061 aatcgtcttg aggcagcgaa gaatgttgcg aatgaattta ttgatggcag gtttcaggac
    15121 cggataggaa tggtaatttt ctctggagat gcctattctc tgtcgcccct caccaccgat
    15181 tataagctgt tgaagcagta cattaaagaa atatcctttg acatgatcga aaaccggggg
    15241 acggcaattg gcagcgctct cgcggttgcc accaacagga tgcgtgaatc tgattcaaaa
    15301 tcgaaggtag taattctact tagcgacgga gacaacacag ccggcaacat tgatcccatt
    15361 accgccgcaa aacttgccca ggcatacggc atcaaaattt ataccattgc cattggtaaa
    15421 gatggccgcg ttccttttgg caaagatttc tttggacgaa cacggtatgt ggaaaatacg
    15481 ctggatgaat ccacattgag agaaatcgct gctattggtg atggtcaatt cttcagggta
    15541 tcggataatc aagccttaca gcaggtattt tcattgatag acaagcttga aaaggcggag
    15601 ataaaggaga caagattcaa agataccacc gatttttatc gaatatatct gcaatgggca
    15661 atcgtgatgg ttctggcatg gatggcgctc aagagttcct ttattttcaa tattcttgaa
    15721 gattaaagtg tccaggcata atagagtaat cttattccaa atcccaataa gataactcca
    15781 accacaatat tcataatttc aataattctt ggcttaatca gaagtccaag tttgttggcc
    15841 atgtatgctt tagtaacgtc agtgactaat acagaaagta aaattgtgcc aaagaatatt
    15901 ttcaaatcaa cgtcatgaaa tccgagatcc acggttgcaa aacccattac tccaagccag
    15961 aagattaaaa cgaaagggct caccgtgttc atcacgaaac ctttgaccag agccctgata
    16021 acgcttttgg gattctttac aaagctcttt tgccggtaag actttttctt cataaacgaa
    16081 taaaatccca gtgcaatcaa gattatacca ccgataattc ccaaatagta tttatattct
    16141 ggcagggaaa ttaattttga aaaccccagg taggaaaata atacaaaaga tgtatcgctg
    16201 attgaaattc caagggccat taccaaaccc gcgatgaaac ccttttcaac acttgtctgg
    16261 atcagcgaaa agaaaacggg gccaatagca aacgagagga ttattccaaa acccaatcct
    16321 tttaaaataa tatccattag cggcgcacct tctcattttg caaatacatg gtccagctat
    16381 tgtatgacga tttgtttagt actctcggaa atttattctg accaccctct tttccctggg
    16441 ttttaagcca attataaaaa aatgcggaag gcagaatttc aaattcgacg gatttaagtg
    16501 ctgcatttct ttcaactttg taatcatcat taagttcttt caaaacttcg tccagtcttt
    16561 ttgccaagac atctgattta accggatcat cgcaaccaat aaaccagtgg tgaccgaata
    16621 acgaaccaac aggcactcct gctactgaaa attcctttat ttcaatatct aattcattag
    16681 aaataatttt tatcgcatga ttcatattgt ctactgacaa atgttccccg caaagactca
    16741 gataatgttt ggtccgaccg gtaattcgga tttccgattc ttccactgat acaaattgta
    16801 tagtatctcc tatcaaatat ctccaggctc ccgcgcacgt ggaaagcaac accgcatagt
    16861 ctgtattttc ctttacatca tcaatcatca tcgcttcatg atcttccctt aattctcctt
    16921 ctgtggtaaa attacgatcg gtgaacggaa caaactcata gaaaattcca ttatttagaa
    16981 ccagtcgcat tgattttcta tgtggcaatg cctgaaacgc caaaaaaccc tcactcgcca
    17041 gataagtttc aatataagaa attggtttcg cgagcagccg ctcaaaaccc ttcctgtaag
    17101 gttcaaagaa aacacctcca tgaacatata tcataagatt tggccagatt tcatggatgt
    17161 tttttacttt ataatgatta atgattttct ccatcagaat ctgaatccag gctggaacac
    17221 ctacaataat tcctatatcc cattcaggtg cttttctggt aatttcttca agcttctcgg
    17281 tccagttagg atttttagca atttttttcc cgggtttata aaagtgttga aaccaaaagg
    17341 gcaactgcga tgcctgtatt ccgcttaaat caccttcaaa ataagtacca ttataattga
    17401 ggctgatact gccacccacc atcaggattc ccttttcaaa taactgggcg ggcagatcat
    17461 atcttgataa tgtcagaatt tggcgaagac tggtttttct gatggcttta ctcatctcgt
    17521 ctgtaatggg aatgtgcttg gatggcgccc cggaagttcc tgaactgaga gcaaaatatt
    17581 ttacgcgacc gggccagcaa acattcttct cacccttaag gcatttatac caccacactt
    17641 caaacatctt gtcataatta tggtaaggaa ctttactctt gaaaacatta taaaacattt
    17701 gacgctccct ggatctgaaa ctctgcaaaa tttccttaaa gtcgtatgtc ttcccaaaac
    17761 tggtagctga agctgttatc aacagttttt ttaattcagt cttttgaaga tcaattggag
    17821 atgaatactc ctgatcaagg ctttcgcgaa ttcgtatccc tttttgtaac aaattgccga
    17881 ctaaaggcat agttagtagt atttttaatt agttcccgtt ttatacatct ttgcagttcg
    17941 aattcagatt ttccaatttg attaattgtc ataaccagga ataaatttag tcatgaatat
    18001 caataaaaac attgcgtatt ttcaaaattt aaccaaaaca aagcattgta aattaattgc
    18061 tgttagtaaa atgaagtctg aacaagacat aatgcatgcc tatgatgcag gtcagcggat
    18121 ttttggtgaa aacagagctc aggaacttgc atcgaaatat gaaaagcttc ccaaagatat
    18181 tcaatggcac atgataggac atcttcaaac caataaggtt aaaattattg caccttttgt
    18241 ttctatgatt gaatcggtgg atagtattaa attgtgccgg gaaatcaaca aggaggccat
    18301 gaaagtaaac cgggtgattc caattttgtt gcaggttcat attgcaatgg aagaaactaa
    18361 atttggcttt aatagtgaag agttgttgga agcaataggt gataatacga ttaagcaatt
    18421 tgaaaatgtt gagcttaaag gattaatggc aatggcaacc ttaacagatg ataatgaaac
    18481 aatccggaaa gaattcaggt ttctaaaaaa tttgtttgac aaggtcgcgt ccttgcaggt
    18541 ttttcccaaa tttaccgaga tttccatggg gatgaccagc gactaccaga ttgcacttga
    18601 agaaggcagc accatgatta gaattggaag tgctattttt ggagaacgaa aataaatggc
    18661 aagcaaagca ataattatcg gaagtatttt gggggctttg gcagtcgcgg ctggggcatt
    18721 tggtgcacat gcgctttctg aatttctgaa cgagtctggc cggagtgtaa cttatgaaac
    18781 cgcggttaaa tatcaatttt atcacagttt actgctaatc ctggctggaa ttttaggtca
    18841 aaaagtttct ataaatatac cggtgtattt aattgttttc ggaatattag ttttttccgg
    18901 ttcgctttac ttattgattt ttaccaatgc ctcctggttt ggtattatag ccccattcgg
    18961 cggggcctcg ctgattgcgg gctggttatc cctggcttac gctgcaagga aactggttaa
    19021 ttaactttct ttttcggcag aacattcgtc acaattgaga ctcttgagat tggtattctc
    19081 gcgttgctgt aaatggtaat aactttgttc tgagcaccag atttgccgac gctattgaac
    19141 tttacggtaa gctctccttt ttcacccact cctaccggct ctctgggcca actggttgca
    19201 gtacatccac atgtgaccgc cacatttgac aaaattaaag gttcatctcc cttattttca
    19261 aatttaaaag tccattcaac tatatcacct tgttcaatgt caccgaaatc atgactttca
    19321 atttcaaatg caattaccgg tcccggagct ttctcttgtg tttccttttg ctcctgttct
    19381 ttcttttgtt cagcagtctg actaaaaccg atagtggcag tcaaaaggaa aaacatagtc
    19441 atcattaatt ttttcatttt ttcaaaaatt tgtattgcaa tttacaaaaa aacattgttt
    19501 gtcgggtcaa ttttaaaaga accttgaatt aaaatttaac aaataaactt ctttcgtact
    19561 ccgggttaaa gcggtgtaca accacctgag ataatcttca tttatcattt cttcagtgag
    19621 atatccttga tctacaaaaa caaggggcca ttgtccccct tgtgatttat gacaagttaa
    19681 tgcataagag aatttaattt gtaaggcatt aaggtaggga tcaaatcgaa gtgctttcgc
    19741 tcgctcaatt tcactatact gtatataatc tttcaaaatt tcctgataga attttttatt
    19801 ttcctccggc gacaggctgg tattttctgt gtgaagggtg ttcagtaaga ccttggcttc
    19861 aaactcttct tcatcgggat aatcaatcat tctcaacctt agcgtagcaa atctatacct
    19921 gtataggtct tcgaaagtga tcaccttcat tacttcaaca aaatcaccat ttgccataaa
    19981 tccggcaggg ttttctttgt agaagtaatt attcttgaca atcatcagga aatcaccagt
    20041 ctcaatttcc tcctctttga aaaaaatctg tcttcggata aattggttat attgagtcgc
    20101 cgctcgattc gacctgcaga taacagtggt attttcaact ccgtatttat catatgcgta
    20161 gcgcaatcca gcttcaagtt tttctgacgt cattttaaaa atatctttaa atcccttcaa
    20221 cctgaatctg atgttaatct ctttggcgga aagagattct cttaaaacag tagcattgtg
    20281 cattatgccc gaattgatat cctgacgcat aacttcctgt agttcaattt cctgaacatc
    20341 cagtttgcat ttcaatttca gataatccat atcaagtgct gcactgatag attggcctac
    20401 aggcggtaat tgggcggagt ctccaatcag cattaattta ttcccttttt tattgaatat
    20461 atactttatc agatcggata atatcccgtt gtcgccgaac tcattgtttt cataaagcat
    20521 actgctttca tcgataataa agacagtttt tttatgatag ttcttctgga gctcaaattt
    20581 tagtaaccct gaaaccgggt ccgcggcttg tttataaatt ttcttatgga tagtgtaggc
    20641 aaccttcctg gcataacctg ataaaacttt tgctgcccgc ccggtaggtg ccagtaaaac
    20701 aaacttataa ttaaacaagg gcagtgtttt tacgatagca cttaccagcg aagttttccc
    20761 ggttccagca taccctctaa gtaatataca atcttttgtt ttggtctctt caagaaaaga
    20821 atcaaataat gaaaacaatt tttgctgtcc ttgagtaggc gtatgggtga atttctgagc
    20881 aagaatggat gatgggttcg gcacttctaa tacatttatt ctttgggtgg aattacaacc
    20941 atacttgttg tcgcttttgc aatcaattct tttttaccat tattctcaga ataaacttca
    21001 gcttcacaaa aattaatctt tcgaccttgg ttaactaccc atccgattgc aaataatcgg
    21061 ctgcctcggc ctggtttaaa aaaggaaact tttatttcgc ccgtcaccac atggtgagta
    21121 gcaggaacta ctgagtaggc cgcaaagcct gcaactatat ctgcaatagt tgtaattaat
    21181 cccccgtgga ttagtccggt ttgctgcaag tgattttgat caatttccat ccagccttct
    21241 gttcttccag gctctattac atctaattca aatccaacaa acttcataaa atgctggttc
    21301 tccagaaatt ttttaattct ggattcgaat tcaggatttt gtacttttat cattgataag
    21361 atttaagtta cggcaaatag aagaaaataa ttttaggata acaacgatgg ttcaatttga
    21421 agaaagctac catggaaaaa ctcgggtgag ggtttgtggc ctgtgcctta tagaaaacca
    21481 attggttctt gtaaggcatt gtggtttggg tgagacgggt acctattggt ctcccccggg
    21541 cggtggaacg aattttcagg aaccaataac cgaagctcta aaaagagaat ttaaagaaga
    21601 aacgaatctt gagatcgagg tgcgtaaata tttatttatt cacgagttta ttggagcgag
    21661 attgcatgca atcgagattt ttttcgacgt atttcgcact ggaggtaacc ttatactcgg
    21721 tgaggatcct gagcatagaa aggacaatca gtggcttcag gaagtaaaat tatttagtca
    21781 ggatgaactg tcggatattc ctcagaatga aaagcatcag atatttagca tggtcagcaa
    21841 tttcaaggat cttcttagtc tgagaggatt tttcaaaagt gaaaataatt ccataaaata
    21901 gcgccctaac cggaatcttt aagccaaaat aattaatatt gcattaaatt gagaagccat
    21961 gaatttgacc aggattcttt ccatatcttt tcttgtagta gcattagctc tttttgcatt
    22021 tcttgtcaat agaataaaat ttgccattga tgaagaaatt cgcatcgaga atgtcgaaaa
    22081 gatagtcatt agaaaacttg aaatgattcg cgtggcacag attgcttatc aatctgtgaa
    22141 cggtcagtat gctaatacct gggagaaatt agttgacttc gtgaatacgg gtgactttta
    22201 tatcattcag cggtctgagg aaattattac actttcatac ggcagagaca gtgtaattgt
    22261 tcatctggat acacttggga cagtacctgt tatagactct ctctttaaga agcagaaata
    22321 cgctgatttc actttggcag atctcccgat tattccggaa agtggaggaa agaaatttga
    22381 cctgttcaca gaaaaaatta ctaaaggtgg aattactgtt gacgtgattg aagttcggga
    22441 cattgccccg gcaaatccga tgcgcagaga aagcaatgaa gcaagaaaca ggaaaccatt
    22501 acgcttcggg tccagaacag acgtaacgac atcaggtaac tgggaataat aatttggagc
    22561 acactgcaat tttctacaag cctatacaca aggtaaagga cgccaaattg agtattgatg
    22621 atttgcatca ttattcactt tcggtgcaaa tgggtgcgcg tgattttcaa ctttgcgtaa
    22681 tagatacccg tgacaaccga tgcctggcta tggaggattt cgccttgtct aaggttagat
    22741 cttacaagca acttacagaa gtcttgaata atctttttga cgaccaccaa tttttaaacg
    22801 ccggcttctg gaaatctgta aaattctcgc taaaaaactc aaagttctcc cttgtgcctg
    22861 ataagttgtt tgatcgcaat aatagttatg attatctcaa tgtaagttgt actgtaaatc
    22921 ccgataaaga aacatttctt tactataaac atatcaagtc taaagcggta aacagttttg
    22981 ccattcctac cgatctgttt aactggatta acaagttgta taagaattcc tcagtcctta
    23041 ttactcacca gtcaagcgcg ttgatcgaag gcgttcttag acaagtcgtc aagtattccg
    23101 ggcccacaat ttatctgttt atcgacagat ttaagctgca catcataaca tctaaaaatg
    23161 gagagcttga gtattacaac caattcatta tttccaggtt ttctgagtac atgaaatata
    23221 taatgatggt gatgcaagga ctgaaataca atcagaaaaa tacggagatt gttctttggg
    23281 gatacctggg aagaaaatcg ccgcatttta ccgagttcag caagttcata aaaaatatct
    23341 cgtttggaga ccggcccgat tatcttaaat tcggatacct gtttgatgat ttacaagatc
    23401 atcactattt cgatttgtat agcacttacc tgtgcgatta atgaaacgaa tagcattatt
    23461 tcccggctcc ttcgatcctt ttaccaaagg ccattacgat attgtcatcc gcggactttc
    23521 gcttttcgat gagataataa ttgctatagg gcataacaca caaaaagtcc ggtacttcaa
    23581 actggaatta atggtaaaaa aaataaaaga gacttttgct aataacaaca gagttacagt
    23641 attagtctat aatgagttaa ctgcagagct tgctcaaaaa cagggtgcca gatacttatt
    23701 gcgaggcctc aggaatacca cagattttga atatgagaac acaatctccc aggtgaaccg
    23761 gcatttaaat agtgagctgg agtcagtatt cttaatcaca tcaccggagt tcgctgcctt
    23821 aagttcttca attattcggg aggtacataa atacggaggc gatgtatccg aactcctccc
    23881 ttatcctatc tgacctttcc atctgaaata acattccata acgtatccca atgacctgta
    23941 cgaatgcagc aaggagtgca aaagttcttt gtgattcatc cacctgattt catcaatatt
    24001 ttcttccttg gccggtttca tatgagtatc ttctatgcat ttcattgaat accacaccgt
    24061 tttcttaaga atattttttt ctcctaagct gtacgtatga taagatgcac aaatcctctt
    24121 ccttggtgat acagatatat tacactcttc ttcaacctca cgcatggcgg cctccataaa
    24181 actttcaccc ttttctactt tgcccttcgg caagtcccac ctccttagtc tgtggatcat
    24241 taagattttg ctccctttct ccactattcc tccggctgca ttaataaatg tataataaga
    24301 ttttaccacc tctttcaccc gtagttcaaa atcaaccaca atagttactg aattcaattt
    24361 tgcaaacggg ctattggtaa ggattttaag gatctttctt agttgttttt cggaaacatt
    24421 tctgattatg acattcccga agaattgagc attggagagg ttattttcaa gagcattaag
    24481 atccatttca aattccaatt cctttttgaa agttttagac ttgattaccc ttaccggaac
    24541 atcattaata aaaatgcgca ttgagctaat ttatcccaag agaaaaaaaa atcttttaac
    24601 atccaataga caatgctatt attgaggaaa ttttcgacta attatattat gaacaatgca
    24661 gaggcgggaa gagttattgc tgcaacttta ttggaaattg gtgcaatcaa gttaaatctt
    24721 aagaagccat ttttgtggag ttctggctgg aaatcaccta tttattgtga taacagactt
    24781 tcattgtcat tccctgcctc cagaactttt attaaagaac attttggcca acttataact
    24841 tcaaagtttc ctgatgccga agtagtcgcg ggagtagcaa ctgccggtat accgcagggt
    24901 gcgctggttg ctgatttgct taaccttcct tttatttatg tcaggtcaaa accaaaagga
    24961 catggaatgg agaatttgat tgagggaaag gttgaaaagg ggcaaaaggt tgttgttgtg
    25021 gaagatttga tctcaaccgg caaaagctca ttaaattcag tggatgcact cagaaaggca
    25081 gggctacagg tactcggact ggttgcaatt tttacctatg gctttccgtt ggcggatacc
    25141 aattttaagg aagccaatgt gcaggcgttc gcattatcaa actatacata cctgcttgaa
    25201 gaagctttag acaaaaacta cattacaaac gatcaactgg tatctttgaa tgggtggcgg
    25261 gtagaccctg aaaaatggga accctaatgc cagaacttct cttccactcc caggccgaac
    25321 aaagcaaagt catattttac agggtcgttg caatcaaatt gtctaagttg gcgcgtaagt
    25381 tcgagggcca ttttcaaatc cgctttcttt ccggaaacca agcctaattt ttttgccaca
    25441 ttttctacat ggatatcaca gggacaaatc agtttcgatg gagatatttt tctccagatt
    25501 ccaaagtcta cttcattgct gtcttttcta accatccaac gcaggaacat gttcagtctt
    25561 ttgcaagccg aatttctaac cggagttgaa atgtgtttcc tggttcggga aggaaaattc
    25621 ggtaatgaac aaaaagtatt atggaaatta gccagaccat tctgaattgt ttcatcgctt
    25681 tcttttacaa caaagagatt ttctaatgac ttattttggg aataatacca cttgaaaaat
    25741 gaaatgaaat aaagggtatc tgtgtaattg aaagtcctgt gcttaaaatt tctgaacctc
    25801 tttaaatcac tctgcgaatg attaattatg aattcatagg gagcatcatc cataagtgaa
    25861 aataattctt tgcatttatt gatgattgtc tttctctggc cccatgacag gatagctgtc
    25921 cacaaagcag taatttcaat gtcctgtttc ttcctgtata aatgaggaat tgaaattgga
    25981 tctgagccaa taaacttcgg attgttatac tcctcaacct tcaggtccag gaaatttttt
    26041 aaatctaaaa gattcttttg agcgatcagg gtacgtagct tatttcaacg cggaattttt
    26101 tatcaattgc acccaggcga tcgtaagcgg ctttggaaag tcttataagc atcccctcat
    26161 tggctccagt atcaggcagg cgacccagca ccctcacaaa tactacctta ttattcatct
    26221 catttcttac ctgcatgatg gtaccaattt tagcggtttt atgcaaagcc agatatttct
    26281 tattgtcttc acttccctca ataattccac ccattccgtt ttcaaccagc cgggtatacc
    26341 cactgctgtt ggtaacggtt tcaggacctg ttctgccaac ataaacaacc tcctccggtt
    26401 ctttttctac ctttgctatg ggtggttgtg ctttgggtgg ttcaactttt tcaacctgga
    26461 ttacatcaga tacttcagga ataatttcag tctcattcga tggagctgca accacattat
    26521 tgttgtcgcg tctcatcccc acgaataatc tctggcccaa ggaaatgcta ttattcacaa
    26581 tattattcca ttgccggatt tccagcacct ggacttcata ttgtctggaa atagcgtata
    26641 aagtctccga tgctttaaca atatgaaaaa tagaatcacc ggcatgagca ggcatttggg
    26701 ggcttccacc tgccgttaca atcaattttt gacccacgtt aagtgcattt gaattgaggt
    26761 tattccattt tatcaaatca tcaactgtta cattatacag ctttgaaatg aagaacagag
    26821 tttcggaagt ctttacaata tgaatttcac cgttttcttc cttttcacga gggatgtacg
    26881 gaattttcag aatctcccct attcctaaac ctgatttagc ttccggattt tgctcaagaa
    26941 tctttgggat aggtactccg tacctccttg ataaggaata aagcgtttct ttttcatcca
    27001 cctgatggat gataaagtaa tgttcacctt ctttttccaa tctcagggag tctaaatttg
    27061 cacaaaaacc agtaattgta aatgatacca ggaagatgct tgtgaggaaa aatctgatca
    27121 tcgactgaaa agatagtatt ttgttcaaaa tcaataacaa agataataga ttaatattga
    27181 aaaacggatt atgaaactct ccaatgaact ataacgccgg gaatccttat ttcgcttata
    27241 tttaagaagt gattttgata gtgaaaatga aaaagttttt catagcgcca ctggctgttt
    27301 ttttatttag tctgggaatg gtttccgggc aaaacgataa gcaaagcaca atcctggtgc
    27361 tggatattaa agatgcaata gatccccgaa tgtcccgact ggtgagacta gccctggaaa
    27421 aagcaacaga tacgactgct gacatacttt taattgaaat ggatacttat ggaggggcac
    27481 tcacagatgc cgatgacatt agaacaatgc tattagagtt caaaaaacca acctgggtat
    27541 ttattaaccg ggatgcggcc tcagcgggag ctctcatatc aatcgcttgt gattccattt
    27601 acatggcatc cggagccagc attggtgcgg caacagttgt taccgctgat ggcagtgcgg
    27661 ccccggataa atatcagtct tatatgaaat ccattatgag atccactgct gaagctaaag
    27721 gtagaaatcc ccagattgcg gaagctatgg tggatcagga aattgaaatt gagggaataa
    27781 cggaaaaagg aaatgtgatc actttttcaa ccagtgaagc gattcagaat ggcttttgcg
    27841 aaggacaaag gaccagcatt gatgagatca ttcagaaaaa tggaataagt aattataatg
    27901 tagttcgcta tgaacgctca actgccgaga aaattattgc ttttttccta aatccctttg
    27961 tgagtggcat cctgctcctt gttattgtcg gaggaattta ttttgaattg caaactccgg
    28021 gagttggatt tcctatactc gccgcgggaa ttgccttgct tctctatctt actccttact
    28081 acctgaacgg cctcgccgag aattgggaaa ttttaatgtt ttttgccggc atcggtctgc
    28141 tggtccttga gatttttgtc attccaggct ttggaatagc aggtatattg ggaattattt
    28201 tcgcgggggg ttcattagtg ctggtaatgc ttaataatga tttctttgat ttctcatttg
    28261 ttgagggcga taaaattgta atggccgtaa caaccacact gggagcttta ataggaagtg
    28321 ttattgttat gtttatcggg ggcgttcgat taaccaatac aaagattttc aaacggattt
    28381 cgttacaaac ggttcaggat aaagaatccg gatataccag cagatttgtc actaccctca
    28441 tgacaggtaa acagggaacg gccttcacca ttcttcgacc cagtggtaaa gtgttgatag
    28501 atggagaagt ttacgatgca tttacccgtg gtgaatacat tgagaaagac gctccggtaa
    28561 ttgtagtcga tgaagaagga acttccttaa aggtaaaagc tgcaccagaa atcaaatctt
    28621 caaagaaaaa gaaaaattga acttgaaaat tgcaggcatc attcccgccc gatataaatc
    28681 caccaggttt cccggcaaag ccttagccga gcttaaagga aaatcaatga ttcaaagagt
    28741 atatgagcaa tctttaaaag caaaaagcct tgattctgtg atagttgcga ccgatgatca
    28801 gcggattttt gatcatgtag ccgggtttgg cggaatggca gtgatgacct cccccgattg
    28861 cagaaatggt acggaaaggt gttatgaagt tattgccggc aatgaacgga aatttgattt
    28921 tataattaat atccagggcg atgagccctt cataagcccg aaccaaatag atcaggttag
    28981 ttcattattc agccttgaca ttcaaatggt gaccctggcc aaaaaaattg acactatgga
    29041 agagttgttt aataaaaatg tcaacaaggt gattttcaca aaggatcatt ttgcaatata
    29101 cttcagccga actccaattc catatctgag aaattttgaa aaggatgcct gggttgaaaa
    29161 tcacgactat tataaacaca ttggaattta tggctacagt gcccgcgttt tgaaagaaat
    29221 cgtcaacctt caacccacac ctcttgaaat tgctgagtcg cttgaacaat tgcgatggat
    29281 tgaaaatgga tatagaatca aaatgggaat aacagatatt gattcacagg gtattgatac
    29341 ccctgatgat ttggaaagaa ttctcaacaa aataaataaa taatggattg ggagtacctg
    29401 gtaattcccc ttaccgctct aattgcctca ctgttaactt ttttttcagg ttttggtctg
    29461 ggcactattc tcacaccggt atttgccatt ttcttcccgg ttgaactggc aattgcttta
    29521 actgcaatag ttcatctgct gaataacctc ttcaaattct tattgattgg tagaaatact
    29581 gttacgcgca ttttcttgaa attcggcact gcgagtgtca ttggcgctat agtgggaagt
    29641 ttgctcctaa ttaccatgag agaattaagt atcaactatt cggtttcaat agcaggtttt
    29701 gcaatccaga caaatcttat gaaggttgtt attgggttat taatactatt ctttgcactc
    29761 tttgaattga ttccaacttt gagaaattac caatttccaa agtcatggtt tgttcctggt
    29821 gggctgctaa gtggtttttt tggcgggata tcaggtcacc agggagctct gcgaagtgct
    29881 tttcttatca aatacggctt ggccaaagag caattcattg ccacaggaat tgctatcgcc
    29941 cttgctgtgg atattgtgcg aattacattt tacggaacca gtttctatga tagttcaatc
    30001 ctggaaatga aaggaattct gtcaattacg gtaatttcag catttctcgg tgcgtccttg
    30061 ggaagacaat tgcttgataa agtaacactt aaatatgtag agcaaactgt aggcggattc
    30121 ttactgataa tggggcttct tattacttta ggaattgttt agagataatc ttcaatggaa
    30181 agttgcgcat aaactcataa ccattaaatt tgagtcattc aacacagcac acatcacaat
    30241 aaaaattaaa tggcttggtt aaaaagtact ctttccagta gcattgggaa aaaagtatta
    30301 atggccctga cagggctttt cctgattctc tttctggtta ttcatttagc aggcaatctt
    30361 caattattga taaatgacaa cggggagagt ttcaatattt acgcgtatac aatgggtcat
    30421 aatccactga tacgagccgt ttcaattctg aattttgctt tcatatttat ccacattatt
    30481 tggtcaattc tgctgacacg gcaaaatcgc ttagcccgcg gaatcaatta tgccgtcact
    30541 gcaagttccg cgaacagcag tctggcttcc cgcaacatgg gtattcttgg attcataatt
    30601 ctaattttcc tggttgttca tataaaaaca ttttggtttg aattcaagtt tgggtcaatt
    30661 ccaaccattt cttacgaagg tgttgaaatg cacaattatt tcagggtggt aaatgaagca
    30721 tatcaaagct ttccttatgt ggctttttac gttgtatgta tgggtttcct tgcattccat
    30781 ctgagtcatg gatttcaaag cgcgtttcaa actttaggat tgaatcatgt gaaatatact
    30841 cccttcatca aaaaggtggg agcaggattt gcgataataa ttccagtact ctttgccctg
    30901 attccagtta tgatgtatct aaaaaattta taataaagca tacatgaacc tagacgcgca
    30961 gattcccgaa gggccgcttg cggagaaatg gaccaaacac aagttcaatc taaagcttat
    31021 aaacccggcc aacaaacgta agtatgatat tattgtggtt ggaacgggtt tggctggagg
    31081 atcagctgca gccagtttgg cggaacttgg gtataatgtg aaagcatttt gctttcagga
    31141 tagtccacga agagcccata gcatcgctgc tcagggtggt ataaatgcgg cgaaaaatta
    31201 tcaaaatgat ggagatagta ttttcaggct tttttatgat accataaaag gaggagatta
    31261 ccgttcaagg gaagcaaatg tttaccgtct cgctgaagta agtgtgaata tcattgacca
    31321 atgcgttgca cagggcgttc catttgccag ggaatatggc ggattgctgg ccaatcgatc
    31381 ttttggaggt gcccaggttt caagaacctt ctatgctcgt ggacaaaccg gccaacaatt
    31441 gttattgggt gcatatagtg cattgagcag acaaatatca gccggtaaag tacaaatgtt
    31501 ccccagaacg gaaatgttgg atttggttat ggttgatggg acctcaagag gcattgttac
    31561 cagaaatctg attaccggta agattgagtc gcatagtgcc cacgcggtag tattggcaac
    31621 aggtggctat ggaaatgtgt tcttcttatc aaccaatgcg atgggttcta atgcaaccgc
    31681 cgcatggagg gcccacaaaa aaggtgcatt atttgctaat ccctgcttca ctcaaattca
    31741 tcccacttgt atccctgttt ccggtcacta ccagagcaaa ttaactctta tgtcagagtc
    31801 actccgtaat gatggcaggg tttgggttcc caaaaaagca aggccaggat ttgctgccag
    31861 ggatgccgtt aacattccgg aagcagaaag agattattat ttagaaacca aatacccatc
    31921 attcggtaac cttgttcccc gggatgtagc ctcaagaaat gcaaaattcg tttgtgatga
    31981 aggccgtggc gtaggtagca gcgggttggc ggtttttctc gattttgctg atgcgatcaa
    32041 gcgagatggt agaaatacta tagaggcaaa atatggaaat ctttttgaca tgtataagca
    32101 aattacgggc gacaatccct atgaaatgcc tatgatgatc tatcccgcgg ttcattacac
    32161 catgggaggt ttatgggtag actataacct gatgtcaagt atccccggtc tatatgcttt
    32221 gggtgaatgc aatttttccg accacggagc aaaccgctta ggtgcaagcg cgcttatgca
    32281 aggtctagca gatggatatt tcataattcc ttatactatt ggggactatc ttgcgcgaac
    32341 ttcatacgac aaaactccgg ttagtcatga tgcttttaaa gaagcggaaa acggagttcg
    32401 ggaatcggta acacgattat taaatattaa agggaataaa accgttgatg aacttcataa
    32461 agaacttggt aaagtgatgt gggattactg cggaatgtca cgcaatgaag aaggcctcag
    32521 gaaagccaaa ggcctggtaa aagaaattcg ggacgatttc tggaataatt taaaggttgg
    32581 tggctccaat gaagaattaa atatgtcttt agaaaaagcc ggccgggttg ctgattacct
    32641 ggagttgggc gaattaatga ttgacgatgc cctggttcgc tccgagtcct gcggaggaca
    32701 tttccgggag gaatctcaga ccgaggaagg tgaagccaaa agaaaagatg atgaatttgc
    32761 ctacgttagc gcgtggaaat acggcggaaa gaatgtcgat gaatctttgg ttcaggagca
    32821 ccttattttt gaaaatgtaa agttgactca acgaagctac aaatagatta tgaagcttac
    32881 actcaggata tggagacaaa aggatgcctt aagtaaagga tcttttaaat cttacagtgt
    32941 ggataacgtt tccggtgaca tgtcttttct ggaaatgctg gatgtattaa atgaagattt
    33001 aactcataaa ggggaagatc cggttcattt tgatcatgac tgcagggaag gaatttgcgg
    33061 gatgtgcggc ctggtaataa atggcaggcc ccacggacct aaatcagctg tcactacctg
    33121 tcagctgcac atgcgaagct tcaaagatgg agatactgtc actattgaac cctggcgggc
    33181 aaaggcattt cccgtaatca aagatttggt tgttgataga ggtgcatttg acaggataat
    33241 ccaggctgga ggatatatat ctgtaaatac cggtggtgtc ccggatgcaa atgaaattcc
    33301 aataccgaag aaagttgcgg atgaagcatt cgattcagct acttgtatcg gatgtggtgc
    33361 gtgcgtagcg gcatgtaaaa atgcatctgc aatgcttttt gtttcagcca aagtttccca
    33421 attagcacta ctacctcagg gaaaacctga gaaaagaacc agggttgaaa aaatggtggc
    33481 acaaatggat cttgaaggat tcggtaattg taccaatacc ggggcttgtt cagctgaatg
    33541 tccaaaagaa atcagccagg aaaacatagc aaggctaaac cgggaatatt tgagcgcgaa
    33601 agtgagttca gataatttat aaatgagatt tctgacagac caataatgaa caataaggta
    33661 tcagaggagc aaaagtcagt aattgccaac agggtaaata atcttcccga atcatttatc
    33721 cgggaaatac ttaaagtaac caccaggcct gatattataa gttttgccgg cgggcttcca
    33781 aatcctaaat tgtttcccgt agaattactt cagaccgtta ccaataaagt atttgttgaa
    33841 gaaggcagag aaggattaca gtatacagtg agcgaaggat ttcagccgtt aaggcacaaa
    33901 atttctgaac gatattcaga caaagggctg catgttaaac ctgaaaatat tctcattacc
    33961 aatggatctc agcagggtct tgatctaacc tgcaaattgt tgttgaaccc cggagacgga
    34021 gtgctggtct gtaatccaac tttcattggc gcgcttcaat ctttttccat ttatgaaccg
    34081 agttattcca gcgtagctat ataccctgat gggcccgatg tagaacaaat agccaacgta
    34141 cttagggaaa aagaaataaa attattctat tgtgttccta attttcaaaa ccctaccggg
    34201 gcaacttttt ctgaagaaaa aaggctgcaa atcaccagat taattcatga aaccgaaacc
    34261 ataatcctgg aagatgatcc atatggagaa attaaattta cttctgcaaa aattccatcc
    34321 tttaaacaac tgctgcctga gcaaacaata ttactgggtt cgttttcaaa aatcgtagca
    34381 ccgggattga gaacaggttg gctggctgca cctgaccacc tgataagaaa attggttatc
    34441 cttaagcagg ccgctgatac tcacaataac cacctggtgc aaagaattct tttccggttt
    34501 ctaaccgaat gtgatgttga aggccacatt gcccgcatat gtgaagtata taaatttcaa
    34561 cgcgacctaa tgatggattg cataaaggag catttcccga aagaagtgaa agttttttct
    34621 ccagacggtg gcatgtttct gtgggctgaa cttcctgagc atatcgatgc ttatgcagta
    34681 tttgaggaag caatcaaaga aaaagttgcc tttgtacctg gaaaaacctt ctaccccaac
    34741 ctggacgtca acaatacttt ccgccttaat ttctctaaca gcaacgaaga caaaatcaga
    34801 aaaggaatag agagccttgg aatcgctatc aaaaaattcc tttagatggg caaaatagta
    34861 attacctgta atccccgggt ttcagaaatt ttagccaggg aggttgaatc attgggcctg
    34921 acggttgatt ctattgaacc aaaaggtgta ataacaaaag gcagtttaga agatacctat
    34981 tatcttaatc ttaaccttcg aacaggaaat aaagtcctgt gggaaattgg aagatttaat
    35041 gcgagaaatg ctgacgagct ttatgccgaa gcaagaaaaa ttgagtggga taagtatttt
    35101 cccctgcaaa aaatgttttc aattgaatca ttcgttaaga atgacactat aaaagatacc
    35161 cggtttgcaa atctgaggct taaagatgct attgccgact tctttgttag taagaccgga
    35221 aaacgcccca attccggagc cgacaaatca gaagtagtac tttttctcca ctggctcgat
    35281 gatgaagttg cattgttcat aaatacctcc ggagaaacta tcgctaagca cggatacagg
    35341 caggctgctg taaaagctcc catgattgaa tccctggcct cggcagtttt aatgtccacc
    35401 aggtgggata tgaattcacc tcttgtgaat cccatgtgcg ggagcggtac actggcaatt
    35461 gaagcggcat tattggcatt aaaccgttgc ccggggcact tcagagaaaa tttttccttt
    35521 atgcatctgc agggttataa tcctcgtgtt tggaaagtga taaaatcaga agcaagaaaa
    35581 gttgagcgaa caggtttggc atttccgata atcgcgtctg ataacagtga agaagccatt
    35641 agtgttgcaa gagaaaacgc gcagtatgcc ggcgtcagca atttgataaa attccaggtg
    35701 tgcgattttt ccaataccct aattcccaag cagaggggtg ttataatcat gaatcctgaa
    35761 tacggcgaaa gacttggaga tgttgagcag ctggaaaaaa cttatgagga aatgggtgac
    35821 ttcctgaagc aaaaatgtac cggttattta gggtatattt ttaccggaaa tcttgacctc
    35881 gccaagaaaa tcggtcttca ggcccaaaga aagatagagt tctttaacgg caaaattgat
    35941 tgccggctcc tggagtatga actctttgct ggcaaaaaac aataatggca ggctatttga
    36001 attagctgta caattgattt aattacggga ggaacaccca tgaaaactat cctccaaaat
    36061 attttttatt cgtttccggt tcaattgcta ttgaaccact ttaggaagaa tcatgttctg
    36121 atggcaattt gggtgatact ctttgccatt gtgaatggaa atataggctc aagtcttggc
    36181 attcattacc tgttcctgga tcctgaatat cttaatagtg tgggttttcg gagtttcctc
    36241 attatgggag tggttattgc aggatttagc gttgcatttc acatcaccgc atatatcacc
    36301 gatggccacc ggtttacctt tcttggtcaa cttgcaaaac ccttctccct ttttagcatt
    36361 aacaacagcg tgatacctat tgcatttctg attttctacc ttattaaagt ggtgagtttt
    36421 caggttgata atgaatatgg aaccaccgct tcgctggtaa gaaatcttgg aggattcatg
    36481 gtgggttaca tatctatggt aacactatta tatatatact tcagaaacac aaataaagat
    36541 attttcaaat acgtcgtttg caaagttgat gaaaaactta agcagaatat taaagttacc
    36601 aggcgtaatg taatggagaa attggatgtt gccaggaaaa aacaaatccg ggttgacaat
    36661 tatttgggaa taagaatgag atttcggaaa gtgccggatt accagggatt ttatgataag
    36721 gccaccatac ttcaggtttt tgatcaaaat cacctcaacc tggtcttgat agaattgttc
    36781 atttttgtac ttcttttgat tattggtgtc ttccgggatt accctgtaat gcaaatacca
    36841 gcggcagctt ccggaattct tttcctttca gtgtttgtaa tgtttgccgg cgccttttct
    36901 tactggttta gaagttgggc tgtcagcgct gtgatcttat taggcatcac attaaatttt
    36961 attgttcagg aaaattttct taagaaaaac tacgaagcct tcgggctcaa ttatgatctt
    37021 ccggaaaagg aatataacct gaaatctttg agtcaaacca attctccagg taacattctg
    37081 gaggactgga gtcaaaccaa agaaattctt gagaactgga ggggcaaatt ccctgaaatc
    37141 aacaagccaa ctatggtatt tatttgtgca agtggcggtg gccaacgggc atccctttgg
    37201 acaatgaagg cactccaggc tgctgatagc gcaactaatg gccagcttat ggaaaatacg
    37261 gtgctgataa caggcgcatc cggcgggcta atcggcgccg gttatttcag agaactttac
    37321 ctgagaaaga agaaaggaga ccctgtcaat ttgcaaaatc attcctacct gcaggatatc
    37381 tcgaatgaca accttaatcc aattattttc agcttacttg tcaatgactt gattgtgagc
    37441 tttcaaaaat tttcctatgc caatatgcaa tatcctaaag acaggggtta cagcttcgag
    37501 gaacaattaa ataaaaatac ccgctatttt cttgataagc ccctctcaac ttataaagaa
    37561 ccagaattga aatcggagat tccaatgatg atcctggctc cgactattat gaatgatggt
    37621 agaaaacttt ttatttcacc ccaaaatgtt tcctacctta acattggatt aattcggcaa
    37681 aatccaggtt tcgatactga aatcgaaggc cttgatttcc acaggttttt tgaagattac
    37741 ggatcaaatg atttgagatt cctgagcgcg ctgcgaatga gtgcaacctt tccatatatt
    37801 actccaagcg taactttacc tagcaaccct cccatcgaaa ttatggattc cggtgttacc
    37861 gacaactttg gcatctctga cgccttgcga tttatttatg cctataaaga ttggatagca
    37921 agaaacaccc gcggagttct tatcctcgct atccgggatt cccaaaagca aaatgaaatt
    37981 ttatcaaccc aaaatttgac cttgatacaa aaattctcca accccatcag cagtatatat
    38041 aacaatttcg aaaatctgca ggatttcaat aatgacaaca aaatcatttt cgctaactcc
    38101 tggtttcagg gagaaattca gaaaattgac ttacaatata ttccccgcat taattcgagt
    38161 aataaacttg agcgcgcttc attaaactgg agattgacat cactggaaaa aaacaatatt
    38221 attgatgcaa taaactcgcc ttccaatcaa aaggcccttg aacttctaaa aaggtctttg
    38281 tcccctaacc cacttatcgc caacgaggat taaacccgga acaattagcg aaaattctcc
    38341 atcctgaagt caaaatatct atttattcct gaaaatgaag ggtttttgga aatcatttca
    38401 gcgaaaatcc ttaattttac cagctcataa cctgggaaat tcatatgccg gtagccgtag
    38461 ccccttataa gcccaagaac cacattcgaa ttgtaactgc agcttcactt tttgatggcc
    38521 atgatgcagc gatcaacatc atgcgaagaa ttattcaaag caccggagtt gaagttatac
    38581 atctgggtca caaccggagt gttcatgaaa ttgtaaatac cgcaatccag gaagatgcgc
    38641 aagccatcgc aatcacttcc taccagggtg gtcatgttga gtatttcaaa tacatgtatg
    38701 atctgttaaa agaaaaagga gcaacaaaca ttaagatatt tggcggaggc ggtggaacta
    38761 ttttacctga agaaataaaa gaacttcagg attatggcat tgcccggata tactctcccg
    38821 atgatgggcg atcgatgggt ttacagggca tgataaatga tttggtgaaa aactcagatt
    38881 acactatcgg agaaaatgta aatggtgagg ctaatgaaat tctgaaaaaa gattcacaag
    38941 ctattgcccg gctaatctca ggagtagaga acttccccga gaaatcaaaa atcttcctgg
    39001 atactgttgt ttcacagata caagaaaaaa ttcctccagt tgtgggaatc accggtaccg
    39061 gcgggtctgg caaatcttcc ctggttgatg aactggtaag aagattcctg atggattttc
    39121 caaataagac catcggaata atatcagtgg atccatccaa gaggaaaaca ggcggcgccc
    39181 tgctgggaga ccgaatccga atgaattcca taaacgacag cagggtttac atgcgatcac
    39241 tggcaacgcg gcaatcaaat ctggctttgt cgaaatatgt caaagaagct gtcaatatac
    39301 ttaaagcggc cagatttgat ttggtaattc tggaaacatc cggtattggc caatcggata
    39361 cggagataat cgaacattca gatttatctc tttatgtaat gacccctgaa tatggcgctg
    39421 ccactcaact tgaaaaaatt gatatgctgg atttcgcgga tataatcgcg ataaataagt
    39481 ttgataagcg cggtgctttt gatgcattac gcgacgtgcg caagcaatac aagcgaaata
    39541 accagctctg ggagtccaaa gatgaagaat tgccggttta cggaacaatt gcatctcaat
    39601 tcaacgaccc gggtaccaac aatctttatg aagcactaat caaattaatt atagataaga
    39661 cgagtgcaga tctaaaatca tccttcgagg caaccaacga aatgtctgaa aaaattttta
    39721 ttattcctcc ggatagggtt aggtaccttt cagaaatttc agaagcaaat cgtaattata
    39781 ctaaatgggt tgaagaacag tatgttatcg cgcagaaagc ctataatgtt catggcacaa
    39841 tagaattatt taaatctgcg gataatgaag ttgtaagaag acttaagagt gaatttgata
    39901 aattaaagct tgagcttgat cctaaaaatt ttaaactgat tgaagattgg cctgcaaaaa
    39961 aagaaagata ttcggctgat gaatacattt acaaagtaag aggcaaagat atcaagctaa
    40021 ccacttatac ggagtcactt tctcacacca ggattccaag agtttcttta cctaagtttg
    40081 aagcatgggg agatatattg agatggttgt taaacgaaaa tgtccccgga gaatttccgt
    40141 ttaccgcggg cgtttacccg ttcaaacgtc agggagaaga tccaaccaga atgtttgccg
    40201 gcgaaggtgg tcccgaaaga acaaacagga gattccatta cctttccttt ggccagcctg
    40261 caaatcgatt gtccacggct tttgattccg taactctgta tggtgaagat cctgattacc
    40321 ggccggatat ctatggcaaa atcggtaact ccggtgtaag tatttgttgc ctcaatgatg
    40381 ccaagaaatt gtattccgga ttcaacctga ttgatcccaa aacatccgta tccatgacta
    40441 tcaatggtcc ggcggcaaca atcaccgcat tttttatgaa tgcagccatc gaccagcaat
    40501 gtgaattata tatccgcgaa aatggactcg aaaaggaagt tgaaactaaa ataagggaaa
    40561 tatttaaggg gaaggaaaac ctgcgaccat cttatcaggg aaaacttcct gaaggaaatg
    40621 atggattggg actaatgctg cttggagtta ccggcgaccg ggtccttcct tcggaagtat
    40681 atcaaaaaat aaaagctcga acgctttctt cagttcgggg aacagtccag gcagatattc
    40741 taaaagaaga ccaggcacag aacacttgta ttttcagtac tgaattttcg ctgaaattaa
    40801 tgggtgatgt tcaggaatat tttattcaaa ataccattag aaatttttat tccgtttcta
    40861 tttcaggtta tcatatagct gaggcaggtg ctaatcctat tactcagctt gctttaacat
    40921 taagcaatgg atttactttt gtagaatatt atatttccag gggtatggat gttgattcgt
    40981 ttgctcccaa tctgtctttc tttttttcga atgggataga tccggaatat tcggttattg
    41041 gaagagttgc ccgtagaatc tggtctaaag cgatgaagct gaaatataat gcgaatgaac
    41101 gatctcaaat gcttaagtat catattcaaa cttccggacg ttccttgcat gcccaggaaa
    41161 ttgattttaa tgatattcgc accaccttgc aggccctata tgctatttat gataattgca
    41221 actcattgca cactaatgca tacgatgaag caattactac gcctactgag gagtcagtcc
    41281 gcagggccat ggctattcag ctaataataa ataaagaact cgggctcgca aaaaatgaaa
    41341 atcctctcca gggttcattc attattgaag aacttactga cctggtggag caagcggtat
    41401 tgtatgagtt cgacaaaata acagaaagag gtggtgttct cggtgcgatg gaaaccatgt
    41461 atcaaagaag caaaattcag gaggaaagtt tgcattacga aacattgaag cactcaggcg
    41521 attatcccat tattggtgtc aataccttcc tttcatcaaa aggctctcct actatacttc
    41581 cccgcgaagt aatccgggcc acacaagaag aaaaggaaaa ccagattgcc actgtcagga
    41641 gtttgcacca tcaaaatgaa atgcttacca aagaaaatct caataacctt aagctggcag
    41701 ccattcaaaa caagaatgtt tttggaatcc tgatggaagc ctccaagtat tgcagcctgg
    41761 gtcaaattac ccatgcactt tttgaagttg ggggtcaata cagaagaagc atgtagcttc
    41821 tattttgtgg agtactccgg aagaaatttt attaggatct cctgtaaatg cgtaaaatct
    41881 ataggtttgg tcaggtaatg gttacaaccc atagctaatg cattttcttt ttgctcatta
    41941 aatgcatcgg cggaaatggc aattattggg acagataaat ttcctgaatt tcggatctct
    42001 ttagttgcct gaagcccatc cataaccggc atatgaatat ccattagcac aatttccgga
    42061 ttgtatttct tgaatgctat tataccttcc tcaccatttt cagcaattat aacccgaaag
    42121 ccattgaggt caagaaatct tttcatcacc tcctgattca tactattatc ttcaacaagc
    42181 aatacggtta caccaggttt gataaacgat gaagtaaact ctttttctgt taattcaatt
    42241 ctttgcgttc ctgcgcgcaa aggaagcttt acttcaaaaa tgcttccttt ccctggttca
    42301 ctttctaaag ttattgaacc actcatcatg tgaaccattt tgcgggtgat cgaaagccca
    42361 agaccggtac cgccatattt acgggtgatt gttccatctg cctgaacaaa aggatgaaag
    42421 atactttcat gcatatgtcc ggggactcct gtcccggtgt cttccacttt tatcgaaatt
    42481 gaattattat cagcttgcac cttcagaaat acttttccaa atggatcagt gaacttaatt
    42541 gcattggtta caagattcat catgatctgg ttggttttgg ttctgtcaag aagtacaaca
    42601 tcaggaatat tatcgtcgta gaaatactgg aagttaatat ttttctctcc tgcctttcct
    42661 ttattaatat gatagattcc tttcactaat tgctttaggt gaaggtcttc ctcatttagt
    42721 atcatttggc gagcttcaat ctttgaaaga tcaaggatgt tgttaataag ttctgaaagg
    42781 ttttcaccac tgatgtgaat tttatcaagt atctccaggg tttctaaatc agggtgcttt
    42841 tgcctggcaa gcatattcag tagctgcgta aatcccagta tggcattaag gggagatctg
    42901 atttcgtgac tcatattggc aaggaactcc gttttagcca tgttggcctt aaccgcctga
    42961 tccttggcaa gcctcatctc atcttgtgcc aactccaggt gtcttattga tagatccagg
    43021 gctttggttc gctcctctac catccgctca agattatttt tgtatttaat aagctcacgg
    43081 tttgcaacta ccatttcatc cttgctttct ttaagaagga attggctttc ttccagtttc
    43141 ttatttttgg tttgagaatc ctgcaggagt atttctaatt tacccaacaa actgtttaca
    43201 ttctgactga agccattaac actcaatatt gataatgaaa aaatagtggt aatcagaatc
    43261 cactgaagta tggaaaaaaa atttgattgc acttcaatct ccggcatgat atccagaaat
    43321 tccaggctca ggaatgtaat tacagggaaa aggtaacaga ggtatttcca gattgttttt
    43381 tcggtttcaa aaaacatggg aggaagagtt ataagagcga aacacagcat ttgccctaat
    43441 ctttcattgc cgagtgattg tgtaaaaaag atgtttgcca ggcagatata gcttatcagg
    43501 caaatttttg ccagcgtgta tcttttcttc cagttaataa atagcggaat caggaatccc
    43561 aggccaatta agcaggaagc actaaaaatg aataaggaat cggtcagaag aaaaaaaacg
    43621 gggctttgaa taaaactggt aacagcaaaa attagcgaga tcctgttaac gagtctgatt
    43681 cttccattgg ctcggccacc caattgggct ctttcaccaa tacctaccaa taaacttatt
    43741 atttccaata ttgacttgat aaattcctgc ataaaactca cgaatcatct aagtaaactc
    43801 atcggggtgt tttcttatca gaaaattata cctttgactt gtactattgt aacattgttt
    43861 tgaatggtaa ttactacacg atacctggaa gatgatttat ttgaagcatc caatgagacg
    43921 ggtgcatcca gcagaattga tatgaggaac aaagaagtta agaaaaatct ctctccggtt
    43981 gaaggactgc tgggatcttt gggtacctgt atcggtggtg atgtaattga catcctgaga
    44041 aaaaggagaa aaaccgtgat tggccttaca ttggttacaa atggaacccg tcgggatcat
    44101 catccgagag ccataactgc catcaggtgc aaagttgtcc tggaatcacc tgatgcaacc
    44161 gttgaagaac tggcaaaagc tgcccaattg gttgtcgaca aatattgttc cgtagcttca
    44221 agccttaaac cttctgtcaa tctgtcctgt gaaattatca ggcctgcgta gtaaatcgcg
    44281 aaaatacccc aagaggcagc tttcttcctg ccctatcttg aaaaaataat tctccagcgt
    44341 ccgacttcag tttgaatatg gaattaacaa gagattctgc caccagggga gataatccca
    44401 ttgaataaag atttaagacc aaaaatcgat ttcgaccatc caataatttt tcgcaaagaa
    44461 tcagcagctc ggggagtaaa tcttccagca gccatttctc cccttttggt cctctgccat
    44521 aagctggtgg atccagaatt attccctgat attttcttcc tctttttact tccctattca
    44581 caaattttaa agcgtcttca ataacccagc taatccccga aagcttactc tgttccatat
    44641 tctctctggc ccaggtaacc acttgcttca ctgaatccag atgaacaacc tccgcgccgg
    44701 cggctctggc ggccagcgaa gcagcgcctg tataggcaaa caaattcaga atggtggttt
    44761 tactattcat tcttttgatg gtatcataaa taaaattcca attatccgcc tgctccggaa
    44821 aaattcccac atgcttaaat gaagttagcc ccaacctgag attcaatttg gaatttttga
    44881 gatgatactg tacccaccat tgctccggca tacctttttt taaagaccat tgccctttgt
    44941 cagcactgcc tttcagctta ttgaattttg catctgcttg ctgatgccaa tccggatcac
    45001 taagtccttt tgaccagata gcctgaggct ccggcctgga aataacatat ttgccaaacc
    45061 gctccagttt ttcatagccc ccgctatcta tcaattcgta atctccccaa accggtgaaa
    45121 caattttcat gaatgattat ttttgattct ttaaaaagta tttttttatc gatttttaac
    45181 cccatgtctg aatatatctc cagcacaaag aatccaaaga ttagaaattt aattcacctt
    45241 aagaaaagcg gcgtcagaaa aaaacaatcc ctgattatta tcgaaggtga aaaagaagtc
    45301 aaaatggctc tggaagcagg agtaaagatt ccaactcttt tcatttgccg ggaatttttg
    45361 aagattgagt tggatcagct cttcagtctt ggcattaaaa ccagtgatat cctgaatata
    45421 agttctgaag tctttaacca tgtcgcctac aggcaaaatt ccggtggaat aataggtatc
    45481 ggcattccta ataatttgca actttcctct ctcaatcctg gcaaaaattc tatttatatc
    45541 atacttgaag ctgtcgaaaa gcccggcaat ctaggtggaa tactaaggac cgcagatgca
    45601 gccggagttt caggtttgat tgtttgcgat cctaaaactg atatttataa ccccaatgtc
    45661 attaggtcca gccttggttg tattttctcg gttaatacag taactgcctc ttctgaggct
    45721 gctctcgcct ggctgcgcca aaaccaggtt atttcttttg ctactcacct cgcaactgaa
    45781 gagttgtact ttgaacagga tttctccgga agaatcgcat ttgtgatggg aacagaatcc
    45841 gaaggcttaa cagatttttg gaaaaccggg gctgataagt tgatcaaaat cccgatgctc
    45901 ggccaggctg attctatgaa tgtttccgtt gccacggcta ttgtactctt tgaagctctc
    45961 agacagcgcg attttgtcgt ttagccactc gttcatctcc ggatagaaaa ttgttcattc
    46021 ttgaacactt ttacctggta atctggtaaa agctacgagt taacttattg tttttaagta
    46081 acttataaaa agtgaaacta aatggcataa gcttggtatt ctatgtaagc aaaggtcaaa
    46141 aacttaaaat aaaatgagag ccaaatacat cttcggaaag atttcattct taatcatatg
    46201 tgggctgttt gtatctttct cacttgtcaa agaattcagc gatctggaat cttcaaccaa
    46261 tggtaaagct gaacctattc agactttaaa aacttctgaa gactcgtctc atgagaacat
    46321 agtaaattta aaattttatg attgtaatga aaagctcatt tacaaaacgc aggttgatct
    46381 caacgatgct tcaaggaaaa aagacaaacg attaatgaaa aatctgaaag caagtgaact
    46441 tataatgaaa gtggatcata cattattctt caggataaat tagttgctaa gggttaagtt
    46501 tggatgcaaa ggaaggttaa ttaccttcct tttgctattt tagggagttt taatttgcag
    46561 accactaatg cccggtaacg aacgtcctga aaaaaagcca agcttaaagg aactttggta
    46621 taccattatt tttgaagcgg aaacccccgc cggtaaaaga tttgacatca ttcttctggt
    46681 tgccattttg ctaagcgtaa tggcggtaat attagaaagc gttagcacaa tcagcatcga
    46741 ttttggtgaa gaattgagaa ttatcgaatg ggtttttacg gttattttca ccatggaata
    46801 cctggccagg ttgtatgtaa ttaaaaacaa aataaaatat gccatttcat tttttggtat
    46861 tgttgattta atgtccatta tccccaccta ccttagttta attctggcag gcactcaaac
    46921 cttgttagta ataagaagtt taagattgct cagagtattc cgcattatga agttggtgag
    46981 atacctggaa gaagccggta atctctcaaa tgcattaaaa gcaagcaaag ctaaaattct
    47041 cgtcttcctg ggaacggttt ttatccttgt gatccttgtt ggtactttga tgtacatggt
    47101 tgagggaccc agaaatgggt ttaccagtat tccaaccagt atttattggg ccgtagtgac
    47161 cctaaccact gtcggttatg gagatattgc tccgcaaaca gtgttgggcc agagtcttgc
    47221 cgcggttgtt atgattctag gctatggaat cattgccgtc cccaccggca ttgtatcggc
    47281 ggaaatggtc aggcaacagc gcacgattga gaaattacca acacaagttt gtggcaattg
    47341 catggaggaa ggtcatgata ttgatgcaat acactgtaaa cattgtggtg ccaaacttta
    47401 aacgttcgtg attcccctgg aaattaaagc tctggacaaa tcgcattggc cggccgttag
    47461 taaaatatat gcggatggcc ttgagacgaa aattgcatca tttgaaacca cctgcccttc
    47521 ctgggaagac tgggatatga atcaccataa attttgccgg ttcgtaattt gtgaagataa
    47581 gaatgctatt ggttggattg cgcttgtgcc tgtttcaaat agaaaggtat atgcgggagt
    47641 tgctgaagtc agcatttaca tatcaaagga atcacagggc aaaggagttg gcaaaatgct
    47701 aatgtcacat cttttaaatg aggtggagaa gcatggtatt tatacgcttc aatgttcgtt
    47761 gtttcctgaa aaccgagtaa gctatgcact gcattctgcc tttggatttc gggaaattgg
    47821 ctatagggaa aaggtggcca aattggaaaa tgagtggaga aatacgatat tgatggaacg
    47881 aaggagtaaa tcagaagagt ttaactaatg aaaaaaatcc ttatcctttg tacaggaaat
    47941 tcttgccgga gccaaatggc tgaaggttat ttcagaaaat
//
LOCUS       DASWHX010000031        19533 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_2981847, whole genome shotgun sequence.
ACCESSION   DASWHX010000031 DASWHX010000000
VERSION     DASWHX010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 19533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19533
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2981847"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            111..1391
                     /locus_tag="VGA21_01845"
     CDS             111..1391
                     /locus_tag="VGA21_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009578448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEX9649278.1"
                     /translation="MKVLKIIFVLILVGSSGWYYMLRVAEFNRRIEIGRRIPAEVVAQ
                     KEPTRVFGMIVDDLLVEQGKIKRNQNLSEILAKHNIDPGRIGELGKASRRVFDVRRIR
                     SNTPYTLICSNDSLHTAKVLFYEPNPIDYVAFHFEDSIRIEMHKRPVEIVEKTAAGTI
                     TSSLFETMAQMGLSPQLTNDFADVFAWQINFFYLFPGDEFKVIFEEKLVEGNSVGLGE
                     ILGASFKHDNKEFFAVYYDQGNGIDYFDESGKSLRKALLKFPLEFTQIISSRYTGRRF
                     HPVQKIYKAHLGTDFPAPTGTPIRSVGDGIVAEAGYTSGNGNYVKIKHNGIYTTQYLH
                     MSKFARGIQRGIKVKQGQTIGYVGSTGLARGSHLCFRFWKNGVQVDALRVDIPPSEPI
                     AEENLTGYFLARDEILQKLSAIEVPGTELHAGGF"
     gene            complement(1528..2070)
                     /locus_tag="VGA21_01850"
     CDS             complement(1528..2070)
                     /locus_tag="VGA21_01850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649279.1"
                     /translation="MRIFFISMSIAFFHPSFAQDKITRNNIYFEAGGAAFFYSINYER
                     LFLRNPRHNLAVRLGATVLPLSRKTERLIHGLPIGISYLAKAKKKYFEAGVSVAGLRD
                     RYYPDSNLGVSDELSEELILIPSVRFGFRSQPAEKNLFWNILFQTSIISANEFHRYSY
                     KDSERYVTPFISVGVGYAFH"
     gene            complement(2123..2416)
                     /locus_tag="VGA21_01855"
     CDS             complement(2123..2416)
                     /locus_tag="VGA21_01855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649280.1"
                     /translation="MNHRQGPNEGGEIYKFSREDVKKQRLKDVNFYILNDWNDREPNR
                     PYENYVIFEYDTWPTGERTATWYHGNRGYTHKYRSADNYYDLDEVYKSDFSFH"
     gene            complement(2382..2840)
                     /locus_tag="VGA21_01860"
     CDS             complement(2382..2840)
                     /locus_tag="VGA21_01860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649281.1"
                     /translation="MKNMTITIIALLLFAGCGDEQQNEQQMSIYGTWQLVEQRFGNVS
                     NSGSWENVPDGYTMEFGSDQKYISSSNAICKSNPSNTGTFNLTTSQDGSIIVIELECE
                     ESENNLFSMEYLYTFKNGYLLFSPTFSCDEGCSYKFKKIVEPQTGAERRW"
     gene            complement(3075..3209)
                     /locus_tag="VGA21_01865"
     CDS             complement(3075..3209)
                     /locus_tag="VGA21_01865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649282.1"
                     /translation="MKHFKHFTIVVLCVWMMACKENEVAKSLDPKFENQSFEENVLGM
                     "
     gene            3384..3518
                     /locus_tag="VGA21_01870"
     CDS             3384..3518
                     /locus_tag="VGA21_01870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649283.1"
                     /translation="MPYQPLFVTPGSSARRIPAGRPILMSGNSEHAFQISTSPAYQLE
                     "
     gene            3548..5842
                     /locus_tag="VGA21_01875"
     CDS             3548..5842
                     /locus_tag="VGA21_01875"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX9649284.1"
                     /translation="MFSRVFLIILSLIIPWFSNAQKVGLVLSGGGAKGLAHIGVIKAL
                     EEKEIPIDFIAGTSMGAIVGGLYAAGYSASEIEKVVLSEEFLNWVNGREDPAYNYYFN
                     KREDNPSFVSVDLKLDSTLSATIASNLANDQIINMILAEWLAQASELADYNFDSLFVP
                     LRVMAADIFTQTEVVIKDGTLNEALRASLTVPLFYRPIQVKGRYLYDGGIYNNFPVDV
                     MIEDFNPDVIIGSNVSIKKFEDYPFAEADKLVNNAILFSVLNNSDVGKLREQDIYIEP
                     DLSSFTPFDFNRAKEMIDSGYLATLNHIDKIRTSVAKLKPEEEVEVERENFRKKYKPL
                     SFGGLRFEGISKRQQSYVSRQFNLKKDYLNLEEIKKGYYRLVGQSYFKDIFPNMIYDR
                     ELQSYFFELRGKPRRAFKVQLGGAISTRQVSNIFLEGRFYHFNRVLFDHSALFYAGSF
                     YQSAAYRSRISFPLGIQFYLEPELIYNHWDYLGMSDFFINASSPTAILQTDRKYGLNL
                     GFPVGSKFKAVIQASHYNNDNLFGNNRDYISSDTLDRVKLKGYRYAIEISRNSLNRKQ
                     YPNSGSRLFLGFDYFLGDEFYEPGNTSVLNVDSEQFHEWYRLKLTAESYFKLGRFSPG
                     YVFETVVTNQPFLTNYTGTLIAAPAFSPNTDSKTLFIQNFRSHSYIAGGLRNVIAVAR
                     NIELRMEGYVFKAFRKIVEAETQLPGYDNSIEHLSFSAAVGFVYHSPLGPVSLGGNYY
                     DDPETQFTVMLHLGYLLFNKRSWD"
     gene            5910..6566
                     /locus_tag="VGA21_01880"
     CDS             5910..6566
                     /locus_tag="VGA21_01880"
                     /inference="COORDINATES: protein motif:HMM:NF024304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX9649285.1"
                     /translation="MKKFTITSRNKVNRVPKRANYDAKTLYAILDNMFVCHVAFILDK
                     QPFIIPMAYGRRGNKIYLHGATSSRLMKILKSGIPVCICVTLLDGVVLARSSFHSSMN
                     YRSAVVFGTASLVEEKKKNAALKIITEHIAEGRWNEARKPNFKELKATSVLEVMIDQA
                     SSKIRTGPPLDEPEDYDLPIWAGVVPLNLLAGKTETDPLLKMKLRIPESVKRIRKEFS
                     "
     gene            complement(6547..7023)
                     /locus_tag="VGA21_01885"
     CDS             complement(6547..7023)
                     /locus_tag="VGA21_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013409542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase
                     family protein"
                     /protein_id="HEX9649286.1"
                     /translation="MNKLNEPEALNSVADFHHTFKHPVEKKPIIPSEERCKLRVALLS
                     EELKELEEAIKDNDITEVADALCDIQYVLSGAILEFGLGEKFKALFDEVQRSNMSKAC
                     NSMDEAVATVTFYKDKDGTECYIKAADGRFLVYRKSDNKTMKSINYSPADLKKILS"
     gene            7089..8525
                     /locus_tag="VGA21_01890"
     CDS             7089..8525
                     /locus_tag="VGA21_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009578444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX9649287.1"
                     /translation="MTVTELKNSEVENLHSVFEKQKSSIADFRKEGISARKVRLKKLR
                     KWILLNEAKIVKAVQADLGKPDAEVKLSEIRPVIAEIRKALLNINEWTGNHYVETPLS
                     YMGTRSYIKYEPKGVALIMAPWNFPFNLTISPLVACLAAGNAAILKPSEKSANTSSLI
                     VKMVGEVFSPKEVYAVTGDSEVAKQLLEFPFNHIFFTGSSRVGKEVMKAAAKHLAGIT
                     LELGGKSPAIVDATCNISDTAEKLVWGKFLNSGQTCIAPDYVLVHSSIKAKLLDKMKN
                     NITRFFGRGSEKLSESPDYGRIIDGNHLGRLRKFMEEAIEKGATVAAGGEIDEVGKFM
                     SPTILENVTLDTMLLKEEIFGPILPIISFDKIEEAVSMVNSKPKPLALYIFSSNKKNI
                     RRILGSTSSGTAVINDCVLQFSHPDLPFGGVNNSGIGKSHGFYGFKQFSNEKPVLIQR
                     TGFTAVKMLYPPYKGFVNWWVNVVTRYF"
     gene            8773..9123
                     /locus_tag="VGA21_01895"
     CDS             8773..9123
                     /locus_tag="VGA21_01895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649288.1"
                     /translation="MADKKVTKEERKMIFDLATEKNFPKETVDYLFQQQEPIGTLGAL
                     SQNQKFEYLYNSICLMKVDRKITQSELVFCEDIAMRMGFRKDVVRMLSDQVEIEKTDS
                     TDFESLKSQVYAYY"
     gene            9286..10023
                     /locus_tag="VGA21_01900"
     CDS             9286..10023
                     /locus_tag="VGA21_01900"
                     /inference="COORDINATES: protein motif:HMM:NF013782.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP thioesterase domain-containing protein"
                     /protein_id="HEX9649289.1"
                     /translation="MRGNRTLFIEEYQIRGNEIDRTGKLSLPGLTNIMQETAGNHAKV
                     LGFSVGELQKKGISWLLYRMQLKVANLPHYGDKIRIETWPSGIRGVYAFREFEIWRES
                     EKLGFASTAWLVFNLSNRKLIKIPREFGEKIPDTEEGPRIDLKRKIVAPEKFDNESKV
                     NVGWQHLDLNNHTNNVYYYSWMMGATPESLHVEKTLYEMDAVFKGETGLGDEIRIESW
                     CLDDKMIYRLSHAGSGKEVMISETIWK"
     gene            10066..11283
                     /locus_tag="VGA21_01905"
     CDS             10066..11283
                     /locus_tag="VGA21_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009284280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX9649290.1"
                     /translation="MVTPSAGYTQKGNYLLKPARVFDGKNMHEGWNVFVSNGIISEVG
                     ANIKPSGTYTTIDLPGTTMIPGMIEGHSHILLHPYNETSWNDQVLKESFAERVARATL
                     HLKNSLLAGFTTLRDLGTEGAGYADVGLKKAVDEGIIPGPRLIISTRAIVATGSYGPK
                     GFADHVDIPLGAEEADAGNIVKVVRSQIGKGADFIKVYADYRWGPSGEAMPTFSEEEI
                     RLMVETAKSGGRYVVAHASSHEGMRRAILAGVETIEHGDGGTEAIFKLMKEKNVAFCP
                     TLAAGDAVLQYNGWKKGSEKEPLRITEKRKSFQLALESGVKIMAGGDVGVYPHGDNAR
                     ELELMVDYGMQPEDVLKAVTSGNAQILHMQDRIGSIKPGMFADLVAIAGDPTKDIKVL
                     RQVKFVMKDGKIY"
     gene            complement(11537..12766)
                     /locus_tag="VGA21_01910"
     CDS             complement(11537..12766)
                     /locus_tag="VGA21_01910"
                     /inference="COORDINATES: protein motif:HMM:NF006769.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent hydrolase"
                     /protein_id="HEX9649291.1"
                     /translation="MSEPKIDPERLMQRMEEMAKIGGLPGGGVERLTLSEEDRHARDL
                     LKSWMENLELQITIDEVGNMFGIFNPNNSSDSPIFLGSHLDTVGKGGKYDGSLGVLAG
                     LEVIETIKESGISVSNPIGLVNFTNEEGVRFTPDMMGSLVLKSGITVEEILRIRSVDG
                     KSVFGDELKKIGYDGPGKVNDFTASAYLELHIEQGPVLEIENVNIGAVEMVQGISWNE
                     VTLHGEANHAGTTPMYLRKDAGFVAASIIQYARQLTKEIAGQVANAGLIEVEPNLINV
                     VPAKVRFTLDMRNQDNVNLKLAEESLKAYAQKIATDENVKLEIKSLVRFDPVHFDKTL
                     INLVENSAKDLGYSVKRMPSGAGHDAQMMAALCPTAMIFIPSKNGVSHSIHEYSSPED
                     IERGANVLLRAAFKLAR"
     gene            12851..15907
                     /locus_tag="VGA21_01915"
     CDS             12851..15907
                     /locus_tag="VGA21_01915"
                     /inference="COORDINATES: protein motif:HMM:NF024893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HEX9649292.1"
                     /translation="MLKKLLYGIGGLFVLVIVAAIAIPIIFKDDIRSAIEIEIEKNVN
                     ADVFFNADLFDITMFRNFPNLTVSLSEFGVINRAPFEGQVLLAVQKFEIEVNLKSVLI
                     DKQMRIKGILLDQPQIYVKVLEDGTANYDIAIASEDTVTQETIEESTSADNMTFGIDR
                     WEIRSAFIEYNDATMPIEAKLTGLTHSGSGDFTLSVFDLKTLTHIDTVAITFDGVNYI
                     DNRSFDADLTLNIDLDQSMYTFKENEIKLNDFALAFDGWVSMPGEDIDMDITYSATDN
                     SFKSLLSLIPAIYTSDFSSINTSGNLSFSGMVKGVFSETSMPAFNLALNVSDAMFQYP
                     DLPTAIRNINLDLRVDNQDGVIDNTVVDLRKMHLDFGNNPVDARVLVKNLKTYPIDAQ
                     ANAQLDLAELSTMFPMEGIELRGKFNLDLKAKGVYDSINSIIPAIDLRMEMKDGYVKS
                     ADFPIPLENIRLSSIVSNPTGKMAETLIRVENFGMKLGEDEIEARMTIQNLDDYAWDV
                     NMNGGLDIGEMMKIFPQEGMTLTGKIMANIKTSGKMSDLDAERYDKLPTSGTISISDF
                     SYTDNEYVTMGFGIKKAVANFDPREIKLNSLIGNMGKTDLELSGNISNYMNYVLKDEV
                     IVGRMNFNSQMVDLNEFMNEEVSDESVAPEDTVALEVVQIPENIDFILAAKMDRILYD
                     DMILDNAKGRIIVKNGVLNLDGFGFEILGGRIVMDGEYDTRNIEKPNFDFVLDINQLS
                     IKESFNTFNTVQTFAPVAKNMTGNYSTKFTISGDLGQDMMPLMNSISGSGLIKIAQAA
                     LKDSQVISGITSLTKLSNSDEVSIKDLALSVSIKDGRLSVKPFDIKLGNYKTNISGST
                     GLEGDLDYVLDMDIPAGEIGSQLNNTLASLTGSAPKDSDVIKLKIGLKGTYDKPRPAL
                     IGSGAQAQVQEKVTQAATDEAKKLVSKELTSDPNAQKAVDSLAKIVEDPEAIKKMAEA
                     AQKKIEDSIKTVLAAKQKAIEDSIKKAAAEEAQKKIKDLFKKKGGN"
     gene            complement(15904..16356)
                     /locus_tag="VGA21_01920"
     CDS             complement(15904..16356)
                     /locus_tag="VGA21_01920"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEX9649293.1"
                     /translation="MQKFFLLVLTVSLIVSCTSSSRQEIADASDSGTDEITTDSGLKY
                     TMIKNGSGPEVENGKKITTHCILTLEDGTLIWSSRDDGREYVFVHGVTSHIPGFEEAL
                     SYMKEGDRLKVHIPSQLGYGPAGRGSIPPNANLIFDIEVLTVGERSDL"
     gene            complement(16359..18311)
                     /locus_tag="VGA21_01925"
     CDS             complement(16359..18311)
                     /locus_tag="VGA21_01925"
                     /inference="COORDINATES: protein motif:HMM:NF024371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HEX9649294.1"
                     /translation="MFKKIIISVWTLLPALSFSQEYKNTEWHEGRERISITPGHEKLP
                     SLYLKDYLLNEYFEEEDDLYIYSTVHKIIRVNNDEGIQRHNRIYVPMYNAVELISIQA
                     RSISPSGGIQDLNSDNIKEIKDDETGNAFRIFAVEGLEAGSEVEYKYTAKVGKRYFGS
                     EFVQESIPILNSEFFLICPEHLKFEFKGYNGYPQVNEEVKDQKRYYSAAVRYIPELEN
                     EEYSAYTASRMRVEYKLAFNFDQGPGEILTWNDATQRIYEVIYGGVSDLDKEAVQKFI
                     SLIEFKEVDIESKIRQIENYVKTHISIQEGALSDIDELAMIIARNYTNTNGIVRLFAA
                     IFKQLEIDSEIVLTNGRDETGFDSEFENWNNLSEYLFYFPGTKKFLSPDKYEYRYGMV
                     PFFLTDNNGLFIKTFSLGNFESAEGRVEYIPAIGADQSFDKMEIDMKFETGMSSVQID
                     FKRTMGGYNALSIQPYYSFIPENDSRTILEDFVRSAAEDAVFQSIQVENGEANLSPLD
                     FPFIIKAKFTSSTIVEKAGSKILFKIGEIIGPQVEMYQVRKRVMPMENDYLRKYIRHV
                     NFTIPDGYVVKNPDDINMEVLHLKDEKAIFGFTSNYTIEGNVLKLVCEEYYNELRCPV
                     EDFEEFRKVINAAADFNKIVLVLEKK"
     gene            complement(18312..>19533)
                     /locus_tag="VGA21_01930"
     CDS             complement(18312..>19533)
                     /locus_tag="VGA21_01930"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HEX9649295.1"
                     /translation="YYISKVKKGTEEHVILGEVKNLFDWYSTFTSHLDSEESPDLRKT
                     VEGLTQDLATEEEKVQAIFYWVQDNIKYIAFEEGMRGFIPHNANYIFSNRYGDCKDMA
                     CITRSMLKIAGINSYFTWIGSRDIPYSYEEVPTPIVDNHMIATYYKDGDPQFLDATSK
                     YTQYGLPSSMIQGKQALVQTGPDSYRLETVPVIDRKVNFKRDSSIIKISNGRVIGNGI
                     LSEGGYEKIFDTYRITFASGTKLDELIKAKLTRGSNKFLLENYNLTNLTNKDLPLEIT
                     YSFNIQDYYKQIGDEIYLNLNLSKPFFNQLIDIERRKYPIETEYKYEHTYITTLEIPN
                     GYEIEHLPRNSEFMNEVAGYSINYELLGSKVILRKTVFVEYLILALEKFPAWNDVINK
                     INEAYTDALILKKK"
BASE COUNT         5989 a         3778 c         3957 g         5809 t
ORIGIN      
        1 atgattccaa acttagacgc tacccgaaat ccggttttat ttcagaatta gtaaaattta
       61 agtatcattg aatgattttt tggattttta aatgatttca cagagataaa atgaaggttt
      121 taaaaatcat ttttgtgctg atcttggttg gatcatcggg atggtattac atgctgagag
      181 tggccgaatt caatagaaga attgaaattg ggcgcaggat acctgctgaa gtggttgcac
      241 agaaggagcc taccagggtt tttggaatga ttgtggatga tctccttgtt gaacagggaa
      301 agataaaaag aaatcaaaat ctgtcagaaa tacttgccaa gcataacata gatccaggca
      361 gaatcggtga acttggcaaa gcttcaagaa gagtttttga tgttagaaga attagatcca
      421 atactcccta tacattgatc tgttctaatg attctttaca tacagcaaaa gtattatttt
      481 acgaacccaa tcctatagat tatgtggcgt tccattttga agactcaata cgaattgaga
      541 tgcataaacg accggttgaa attgttgaaa aaacagcagc gggtacaatt acgagcagtc
      601 tttttgagac tatggctcaa atgggtttgt caccccagct aactaatgat tttgcggatg
      661 tatttgcctg gcaaatcaat ttcttttact tatttcccgg tgatgaattt aaggtaattt
      721 tcgaggaaaa gctggtggag ggcaattcag tgggacttgg tgaaatattg ggggcaagtt
      781 ttaaacacga taacaaagag ttttttgcag tttattatga tcagggaaac ggtatcgatt
      841 attttgatga gtcaggtaag agtttaagaa aagctttatt gaaattcccc ctggaattca
      901 ctcaaataat aagttcaaga tacaccggca ggcgttttca tccggttcaa aaaatttata
      961 aagctcacct gggaaccgat tttccagctc ctaccggaac ccccataaga tccgtggggg
     1021 atggtattgt tgcggaagct ggatacacaa gtggtaatgg aaactatgta aagatcaagc
     1081 ataatggtat ttatacgact caataccttc acatgtcaaa atttgcacga ggaatacaac
     1141 gaggcattaa agtaaaacaa ggccagacaa ttggctacgt gggcagtacc ggattagccc
     1201 gtggttcgca tttgtgtttc cgtttctgga agaatggtgt gcaggttgat gcactgagag
     1261 tagatattcc tccatctgaa cctatagcag aagagaattt aaccgggtat tttttggccc
     1321 gtgatgaaat tctgcaaaaa ctcagcgcga tagaagtacc gggtacggag ttgcatgcag
     1381 gaggttttta gaaaaatagc caactccttt cgttaggctc cccaggtttt taagatttga
     1441 tcagttcttc tggcaggtgg ttatattaat tatctatagc ccgctgggaa cgatttcaaa
     1501 taatttggag tttaagtaat ctggttttca gtgaaacgca tacccgactc cgacgctgat
     1561 aaaaggagtt acgtaccgct ctgaatcttt gtaggaataa cgatggaatt catttgctga
     1621 aataatagat gtttggaaaa gaatgttcca gaaaagattt ttctcagccg gttgactccg
     1681 gaaaccgaaa cgaacggagg gtattagaat caactcttcg cttaactcat cacttacacc
     1741 caaattggaa tcggggtaat agcgatctct caagccagcc acagatacgc ctgcttcaaa
     1801 atactttttc tttgctttag ccaggtacga tatcccaatg ggcagcccgt ggatcaatct
     1861 ttcggttttt cgacttaaag gcaacacggt agcgcctaat cgtacagcca ggttatgtct
     1921 gggatttcgt aaaaataacc tttcataatt gatggaataa aaaaaggcgg ccccgcctgc
     1981 ctcaaaataa atattgtttc tggtgatttt atcctgggca aaagaaggat ggaaaaaagc
     2041 aatagacatg gatatgaaaa aaattctcat aaaattctaa ttaattataa cttgcctgcc
     2101 attcgataca tgcattattt acttaatgaa aggaaaagtc agatttgtaa acttcatcaa
     2161 gatcgtaata gttatcagca gaccgatatt tatgagtata gcccctattt ccatgatacc
     2221 aggttgctgt tctttctccg gtcggccaag tatcatattc aaaaatcacg taattttcat
     2281 agggtctgtt tggttccctg tcattccagt cattaagaat ataaaagttt acatctttga
     2341 gcctctgttt ttttacatct tctctgctaa acttgtaaat ttcaccacct tcgttcggcc
     2401 cctgtctgtg gttcaactat ttttttaaat ttgtaagaac atccctcatc acatgaaaat
     2461 gttggggaaa atagcagata accattttta aaagtgtata aatattccat tgagaataaa
     2521 ttattctcac tctcctcaca ttctaattct attactatga tacttccatc ctgactagta
     2581 gttaaattaa atgtccctgt attagaggga ttacttttac aaattgcatt actcgaagaa
     2641 atgtattttt gatctgagcc aaactccata gtgtagccat ccggaacatt ttcccaactg
     2701 cctgaattac taacattacc aaatctctgc tctaccaact gccaagttcc ataaatggac
     2761 atttgttgtt cattttgttg ttcatctcca catcctgcga agagaagcaa cgcaataatt
     2821 gtgattgtca tatttttcat tgcctttttg tttagttttt aattattctg gtctggaagg
     2881 ttcttccttc gactgtttgt atttttaaat aattaattcc acgatttaga ttttctgtgt
     2941 ttagaacgtg agatgttccc gaacaggttt cactgcaaat cggattgccg gaattatcaa
     3001 ttattgtaac ccttgaaatt tcatctgatg agatagtggt agtttcttga gaactgaaag
     3061 ctgctatcga gttgtcacat cccaagcacg ttctcttcaa acgattgatt ttcaaatttc
     3121 gggtctaaag attttgccac ttcattttct ttgcaggcca tcatccatac acacagtact
     3181 actattgtga aatgtttaaa atgtttcatt ttgttgcttt ttgaaagcat aaataacgat
     3241 tgtatattta aaaagaaata ctatatatta ctatacttat aaaataatct gtaaataggg
     3301 tttaattaat aattaacaga cttttttgaa ttagatgatt ttgcgcgctt tcttcaaaaa
     3361 aaagagaaga aaattctctt ccaatgccgt atcaacccct gttcgtcaca ccaggttctt
     3421 ccgccagacg gattccggca ggcagaccta tcttaatgag cgggaattct gagcatgcat
     3481 ttcagatttc cacttcacct gcttatcaat tggaataaaa tccattaatt ttgagaagtt
     3541 ggaaagaatg ttttcacgag tttttttaat aatactatca ctaattattc cttggttctc
     3601 caacgcgcag aaggtaggac tggttttaag tggtggaggt gccaaaggac tggcccacat
     3661 tggggtgatc aaagctcttg aagaaaagga aattcccatc gatttcatcg ccggaacttc
     3721 catgggcgca atcgtcggag gactttatgc cgcggggtat tcggcatcag aaattgagaa
     3781 agtagtactt tcagaagaat ttttgaattg ggtaaatggg agagaagacc cggcttacaa
     3841 ttattatttt aataagagag aagataatcc ttcatttgtt tcagtcgacc tgaaacttga
     3901 ctcaaccctt tcggcaacca ttgcctctaa tcttgccaat gatcaaatta ttaatatgat
     3961 tctggctgaa tggctggccc aggcatcaga attagctgat tataatttcg atagtctatt
     4021 tgttcctcta cgggtaatgg ccgcggatat ttttacccaa acggaagttg tgattaagga
     4081 tggaacactc aatgaagcac tcagggcttc tcttaccgtt cctttatttt accgacccat
     4141 acaggtaaaa ggaaggtact tatacgatgg aggtatttat aataactttc ccgttgatgt
     4201 aatgatagaa gactttaatc ctgatgtaat tattggttcc aacgtgtcaa taaagaaatt
     4261 cgaagattac ccatttgctg aagcagataa gcttgttaat aatgcgatcc ttttttcagt
     4321 attgaataat tcggatgtcg gtaaacttag agaacaggat atatatatcg agccggattt
     4381 aagtagtttc actccgtttg attttaacag ggcaaaggag atgatcgaca gtgggtacct
     4441 ggccactctc aatcatatag ataaaataag aaccagtgtt gccaaactta agccggagga
     4501 agaagtagaa gtagaaagag aaaatttcag aaaaaagtac aagcctctct catttggtgg
     4561 tttgagattt gaaggaattt ctaaaagaca acaatcctac gtgagccgtc aattcaattt
     4621 gaagaaggat tatcttaatc tcgaagaaat taagaaggga tattacaggc tggtaggaca
     4681 gtcatacttc aaggacattt ttccaaatat gatttacgac agggagctgc aatcttattt
     4741 ttttgaacta cgcggcaaac caaggcgagc gtttaaagtt cagctggggg gcgctatcag
     4801 tacaagacaa gtaagcaaca tatttctgga aggaagattc tatcacttta acagagtgtt
     4861 gtttgatcat agcgcacttt tttatgcagg aagtttttac cagtcggctg cctatcgctc
     4921 aaggataagc ttccctttgg ggattcaatt ttatcttgaa ccagagctga tttataatca
     4981 ctgggattac ctcgggatga gcgatttctt tattaatgct tcttcaccta ccgcgatatt
     5041 acaaactgac agaaaatatg gactaaatct tgggtttcct gtgggctcca aattcaaagc
     5101 agttatccag gccagccatt acaataatga caaccttttt ggcaacaacc gggattatat
     5161 ttccagcgat acacttgaca gggtaaaact caaagggtat cgatatgcta tagagatttc
     5221 aagaaactca ttaaacagaa agcaatatcc aaatagcggt tcaaggcttt tcctgggttt
     5281 tgactatttc ctcggagatg agttttatga gcccggaaat acatccgtcc ttaatgttga
     5341 ttcggagcaa tttcacgaat ggtacagact taagcttacc gcggagagct atttcaaatt
     5401 aggtcgtttt tctccaggct atgtttttga gacggtggta acaaaccagc cattcctgac
     5461 aaactatact ggtaccttga tagcggctcc tgctttttct ccaaataccg atagcaaaac
     5521 tctattcatt caaaatttcc gttcccatag ttacatcgcc ggaggtttga gaaatgtgat
     5581 agcggtagca agaaatattg aactgcgaat ggaaggctac gtatttaaag ctttcagaaa
     5641 aattgtggag gcagagactc agctacccgg gtacgacaat agcatagaac acctcagctt
     5701 ttcagcagct gtaggttttg tctatcatag cccattgggt ccggttagtc tgggcgggaa
     5761 ctactatgat gatcccgaga ctcagtttac ggtaatgcta catcttggct atttgctttt
     5821 taataaacgc tcatgggatt agattacttt taaaaccatt tcaaaaaaac catttatatt
     5881 cggccttctt tttgatcaag aaccaatcaa tgaaaaaatt tacaattacc tcgagaaata
     5941 aagtcaatcg ggtccccaaa cgcgcaaatt atgatgcgaa aactctttat gccattctgg
     6001 ataatatgtt tgtttgccat gtggctttta ttcttgacaa gcagcctttc attattccaa
     6061 tggcctatgg cagaagaggg aacaaaatat atttgcatgg agctaccagc agcaggttga
     6121 tgaaaatcct gaaatcagga attcccgttt gtatttgtgt tacccttctg gatggtgttg
     6181 ttttggctcg ctctagcttt cacagctcca tgaattacag gtcggcagtt gtattcggaa
     6241 cggccagttt ggtggaagaa aagaaaaaaa atgcggcatt aaaaattatt acagagcata
     6301 tcgcggaggg tcgctggaat gaagcaagga aaccaaactt caaggagtta aaagcgacca
     6361 gtgttttaga agtcatgatt gatcaggcat cctcaaaaat cagaacaggt cctcctttag
     6421 atgaaccgga ggattatgat ttacctattt gggcaggagt ggttccttta aatctcttgg
     6481 caggaaaaac agaaacggat ccgcttctga agatgaaatt acgaatacct gaaagcgtaa
     6541 agagaatcag gaaagaattt tcttaagatc tgccggagaa tagtttatgg atttcatggt
     6601 cttgttgtcg gattttctgt agacaagaaa tctaccatcg gctgccttga tatagcattc
     6661 tgttccatct ttatctttat aaaatgtaac agtagccaca gcttcgtcca tggaattgca
     6721 ggctttgctc atattggatc gctgcacttc atcaaaaaga gctttgaatt tttctccaag
     6781 tccaaattcc aaaattgcgc cgctcagcac atactgaata tcacaaaggg catcagcaac
     6841 ttctgtaatg tcattatctt ttattgcttc ttccagctcc ttcagctcct cacttaacaa
     6901 ggcaactctt aatttacatc gttcttcaga gggaatgatc ggtttctttt cgacaggatg
     6961 tttaaaagta tggtgaaaat cggccacact gttcagggct tcaggctcat ttaatttgtt
     7021 catcttgcaa ttttaagtaa tcaatcttgt aaagatatat tatatttagt tttactcaat
     7081 tgttaacgat gactgtgaca gaactgaaga attctgaggt tgaaaatctc cattctgtat
     7141 ttgaaaaaca gaaatcatca atagcggatt ttagaaaaga aggaatttct gcaagaaagg
     7201 ttcgcttaaa aaaactacgt aaatggattc tgctcaacga agctaaaatt gttaaagcgg
     7261 tgcaggcgga tttgggaaaa ccagatgcgg aagtaaaact ctcagaaatc agaccggtaa
     7321 ttgcagaaat acgaaaagca ctgctgaaca tcaatgaatg gacgggaaat cattatgtag
     7381 aaacacccct atcctatatg ggtactcgtt catacattaa atatgaaccg aaaggtgttg
     7441 ccttgattat ggctccatgg aatttcccgt tcaatctcac tattagtccg ctggtggctt
     7501 gcctggctgc tggcaatgcc gcaatcctga aaccctcgga aaaatcagca aacacttctt
     7561 ccttaattgt taaaatggtg ggagaagtat tcagtcccaa agaagtttac gcggttacgg
     7621 gtgattccga agttgccaaa cagttgctgg aatttccatt taatcacatc tttttcaccg
     7681 gtagttcccg ggttggaaag gaggtgatga aggccgccgc caaacatctg gcaggaatta
     7741 ctctggaatt aggtggcaaa tccccagcca tcgtagacgc gacatgcaat atttcagaca
     7801 ctgctgagaa attggtttgg ggaaaatttt taaactcggg tcaaacctgc attgcaccgg
     7861 attatgtgtt agtgcacagc tcaataaaag caaagctgct tgacaaaatg aagaataaca
     7921 tcaccaggtt ttttggccgt ggatcagaga aactttcaga aagccctgac tacggcagaa
     7981 taattgacgg taatcatctc ggccggctcc gtaaatttat ggaagaggcc attgaaaaag
     8041 gagcaacggt ggccgcgggc ggtgaaattg acgaagttgg gaaattcatg tctccaacaa
     8101 ttcttgaaaa tgtgactttg gatacaatgc ttttgaaaga ggaaattttt ggcccaatac
     8161 tgcccattat ttcatttgat aaaattgaag aagctgtaag catggtcaat tctaaaccaa
     8221 aaccattggc gctttatatt ttctcttcaa acaaaaagaa tattaggcgg attcttggat
     8281 caacaagttc cggtactgcc gttattaatg attgcgtact tcaattctct catcctgatt
     8341 taccttttgg gggagttaat aatagtggca ttggtaaatc ccatggattt tacggattta
     8401 aacaattttc taatgagaaa cccgtgctga ttcaaagaac cggttttacc gcagttaaaa
     8461 tgttataccc gccctacaag ggatttgtaa actggtgggt aaatgttgta accaggtatt
     8521 tctgattaaa acttttcgga aactatccgc tcatctggaa ttcggagggc aggaaaaaac
     8581 cagaattggg caggcaaaag ttacataaat gttacttctg agtattaaca ttagctaaat
     8641 caaccagatg attaaagaaa attgtcaatt acgctttgat tttatttaat tttatttaat
     8701 tttcattcct ttttctcttc acaaatggat atagtaacca gaaaacagat caatattctc
     8761 atacgcctag cgatggcaga taagaaagtg acgaaagagg agagaaaaat gatctttgat
     8821 ctggctacag agaagaattt tcccaaagaa accgtcgatt acctgtttca gcaacaagaa
     8881 cccattggaa ccctgggagc attgtcacaa aatcaaaagt ttgagtatct ctacaattcc
     8941 atttgcctga tgaaagtgga ccgaaagatt actcaaagtg aattagtctt ttgtgaagac
     9001 atcgcgatgc gaatgggttt tagaaaagat gtggtgagaa tgctgtcgga tcaagttgaa
     9061 attgaaaaga ctgactctac agattttgaa agcctaaaga gccaggtata tgcctattac
     9121 taattcttta ctgcgaacga gaaattattc gcaaagctaa attcagattc aaaccagtca
     9181 aaaattcctt cagtcaaagg catccatccc caacctgatt ttgaataatt tggaagttca
     9241 tgcctgaatt ttcatttatt aaaaaggatt gcagtatttt aaggcatgag aggtaataga
     9301 actttgttta ttgaagaata tcagattcgt ggaaatgaaa ttgacaggac tggaaaactt
     9361 tctttgcctg gtctgactaa tattatgcag gaaacggccg gtaaccatgc caaggttctg
     9421 ggtttttccg ttggagagtt acaaaagaaa gggatctcct ggcttcttta cagaatgcaa
     9481 ttaaaggtcg cgaacttgcc gcattatggg gataaaataa ggattgaaac ctggccaagc
     9541 ggaatccggg gagtatatgc atttcgtgaa tttgaaatct ggcgtgagag tgaaaaactg
     9601 ggatttgcat ctaccgcttg gcttgttttc aatttaagca atagaaaact tataaaaatt
     9661 ccgcgtgagt ttggggagaa aattccggat acggaagaag gtccgcggat tgatcttaaa
     9721 aggaaaattg ttgctccgga gaaatttgac aatgaaagca aagtaaatgt aggctggcag
     9781 catttggatt tgaataatca tacaaacaat gtctattatt attcctggat gatgggcgcg
     9841 acacccgaat ctcttcatgt tgagaaaacc ctttatgaaa tggatgcagt cttcaaaggt
     9901 gaaacgggac taggcgatga aattcgaatt gaatcatggt gtctcgatga taaaatgatc
     9961 tacagattat ctcatgcagg atcaggaaaa gaagtcatga tttcagaaac tatatggaag
    10021 tagaataata tgaaacaact ggtaataatc gtatttatac ttaacatggt aacaccttct
    10081 gccgggtata cacaaaaggg aaactattta ttaaaacctg caagggtttt tgatggaaaa
    10141 aatatgcatg aaggttggaa tgtttttgtt tccaatggaa taatatcaga ggtcggcgcg
    10201 aatataaaac catctggtac atatactacc attgaccttc ccggaacaac catgattccc
    10261 ggaatgattg agggtcattc acacattttg cttcatcctt acaatgagac aagctggaac
    10321 gatcaggtac ttaaagagtc gtttgcagaa agagttgccc gcgcaacttt acacctgaag
    10381 aattcattgc ttgccggatt caccaccctg cgtgatttgg gaactgaagg ggcaggttat
    10441 gctgatgttg gtttgaaaaa agctgttgat gaaggaataa ttcccggacc cagattgatc
    10501 atttcaaccc gtgcaattgt agccaccgga agttacggac caaaaggttt tgcagatcat
    10561 gttgatattc cccttggagc tgaggaagct gatgccggta atattgtaaa ggtagttcga
    10621 agccaaatcg gcaagggagc tgattttatt aaagtgtatg ctgactatcg atggggacca
    10681 tcaggcgaag ccatgcctac tttttccgaa gaagaaatca ggttaatggt ggaaacagca
    10741 aaaagcggtg gcagatatgt ggtggcgcat gccagctcgc atgaaggaat gcgaagagca
    10801 attttggccg gcgtggaaac cattgaacac ggagatggcg gaaccgaagc gattttcaaa
    10861 cttatgaaag aaaagaatgt tgcattttgc ccaacccttg cagccgggga tgctgttttg
    10921 caatacaatg gatggaaaaa gggatcggaa aaagagccat taagaatcac cgaaaaacgc
    10981 aaaagtttcc aacttgcatt ggagtccggt gtaaaaataa tggctggcgg agatgtaggt
    11041 gtttaccctc atggtgataa cgcgcgcgaa ctggaattga tggtagacta cggaatgcag
    11101 cctgaggatg ttttgaaagc ggttacttca ggaaatgcgc aaattctgca tatgcaagac
    11161 cggattggaa gcataaagcc gggcatgttc gcggatctgg tggctattgc aggagatccc
    11221 acgaaagata taaaagtctt aaggcaagta aagtttgtga tgaaggatgg aaagatttat
    11281 taaagcattt ttcaactttt gcagagaaga acgggctaag ccagataaac acctttaacc
    11341 aatttcacaa gattgattcc ccagtatcca ctccatggta gtctgttttt gtcagacccc
    11401 cctccgtggt ctgtcacccg acagaccact aagccaaata aaaacgattc cccagtatcc
    11461 actcactcca ggttgtctac cccattccgt agtttgtccc ccccactaaa tcaccaaacc
    11521 agacttctca cctttactat ctggcaagct taaaggccgc ccgtaacaat acattcgcgc
    11581 cgcgctcaat atcctcagga cttgaatatt catgaatact atggctgaca ccatttttgc
    11641 tgggaataaa aatcatagca gttggacaaa gcgctgccat catctgagca tcgtggccgg
    11701 caccactggg cattcgtttc acagaataac caaggtcctt cgcggaattt tcaaccaaat
    11761 tgatcaaagt tttatcaaag tgcacaggat cgaaccgcac tagtgactta atttccagtt
    11821 tcacattttc atccgtggct attttttgcg catacgcctt taaactttct tcagctagtt
    11881 tcagattaac attatcctgg tttctcatgt ccaatgtaaa tctgacttta gccggcacca
    11941 catttattaa attcggctcg acttcgataa gtccggcatt ggctacctga ccggcaatct
    12001 ctttggtaag ctgcctggca tattgaataa tggaagcggc aacaaaaccg gcatctttac
    12061 gcaaatacat cggcgtagtt cccgcatggt tcgcttcacc atgaagcgta acctcattcc
    12121 agctaattcc ctgaaccatt tcaaccgctc ctatattcac attttcaatt tccagaaccg
    12181 gaccttgctc aatatgaagc tcaaggtagg cagaagcagt aaaatcattt acttttccgg
    12241 ggccatcata accgattttc tttaattcat ccccaaaaac agatttccca tccacagatc
    12301 taattcttaa tatctcttcc acagtaattc cactctttaa gaccaggctt cccatcatgt
    12361 caggagtaaa acggacgcct tcctcattgg tgaaatttac caacccaatg ggatttgata
    12421 cagatattcc ggattctttt atagtttcaa taacttccag gcctgccaaa acacccaggc
    12481 ttccatcgta ttttcctccc ttacccactg tatccagatg agagcctaaa aaaattggtg
    12541 agtcagaaga attattcgga ttaaagatgc caaacatgtt gccgacctca tctatcgtaa
    12601 tttgcaattc aagattttcc atccaacttt tcagcaaatc ccgcgcatgc ctgtcttcct
    12661 cactgagtgt aagcctttca acacctccac ctggcaagcc tccgattttt gccatttctt
    12721 ccattctttg cattaatcgt tcaggatcga tttttggttc actcatttgc ttaaatcata
    12781 atgtagggga aacgaaatta taaattaatg tttgtaaatt gcctatcctt tcacctaaaa
    12841 taataaagcc atgttgaaga aattattata tggaatcgga ggcttattcg ttctggtaat
    12901 cgtcgcggct atagcaatcc caataatctt taaagacgat attcgtagcg ctattgaaat
    12961 tgaaattgaa aaaaatgtta atgcagatgt ttttttcaat gcagatctct ttgacattac
    13021 catgtttaga aatttcccca atctaactgt atcattgagc gagttcggcg tgattaaccg
    13081 tgctccattt gaagggcaag tgcttctggc ggtacaaaag tttgagatag aagtgaatct
    13141 caagagtgtt ttaattgaca aacagatgcg gattaaagga attcttcttg atcaaccgca
    13201 gatctatgtg aaagtgttgg aagacggaac cgctaattat gacattgcca ttgcatcgga
    13261 agatacagta acgcaggaaa cgattgaaga atccacctca gccgacaata tgacgtttgg
    13321 gatagatcga tgggaaatcc gaagtgcatt cattgaatat aatgatgcga cgatgcccat
    13381 cgaagcaaag ctgactggac tcactcattc cggcagtggt gattttaccc tttcggtttt
    13441 tgatttgaaa acccttaccc atatagatac ggttgcaata acttttgacg gagttaatta
    13501 cattgacaat cgatcttttg atgcggacct taccttaaat attgatctgg atcagtcaat
    13561 gtatacattc aaagaaaatg aaattaagtt gaatgacttt gcactggctt ttgatggatg
    13621 ggttagcatg ccgggtgaag acatcgacat ggatataaca tattctgcca cggacaactc
    13681 ctttaaaagt ttgctttccc tgattccggc aatttatact tccgacttca gctcaattaa
    13741 tacctccggg aatctcagtt tttcaggaat ggtgaaggga gtattttctg aaacaagcat
    13801 gccggcattt aaccttgcac taaacgtgag tgatgcaatg tttcaatatc ctgatctgcc
    13861 tacggccata agaaatataa atttggattt aagggttgat aatcaggatg gcgtaattga
    13921 caacacagta gtagatttac gtaagatgca tttagacttt gggaataacc cggttgacgc
    13981 cagggttctg gtaaagaatc tgaagactta tcctattgat gctcaggcta acgctcaact
    14041 cgatctggcc gaactttcca ccatgttccc aatggaaggc attgaattgc gtggcaaatt
    14101 caacctggat cttaaagcaa aaggtgtata cgacagtata aattccataa ttccagccat
    14161 agatttgcgc atggaaatga aggacgggta tgtgaagtca gcagattttc caatccctct
    14221 tgaaaatata aggcttagtt ccatagtgtc aaatcctacc gggaaaatgg cggagacgct
    14281 aatcagggtt gagaattttg gtatgaaatt gggcgaagat gagatagaag ccaggatgac
    14341 tatacaaaac ctcgatgatt atgcctggga tgtgaacatg aacggcggtc tggatattgg
    14401 cgagatgatg aaaatatttc ctcaggaagg aatgactctt accggtaaaa taatggcaaa
    14461 tataaaaaca tcaggaaaaa tgtctgacct ggacgcggag cgctatgaca aattgcccac
    14521 cagcggaact atttccattt ctgacttttc ttatactgac aatgaatatg taaccatggg
    14581 ttttggcatt aagaaagctg ttgcaaattt tgatcccagg gaaataaagt tgaattcact
    14641 gattggcaat atgggaaaaa ccgatctgga actttcagga aacatcagca actatatgaa
    14701 ttatgtatta aaagatgagg taattgtggg ccgaatgaat ttcaattctc aaatggttga
    14761 tctaaatgaa ttcatgaatg aagaggtgag cgatgaatca gtggcaccag aagataccgt
    14821 ggcactggag gttgtgcaga ttccggaaaa tattgatttc attcttgccg caaaaatgga
    14881 tcggatacta tatgatgaca tgatccttga taatgccaaa ggtagaatta tagtaaagaa
    14941 cggtgtgttg aatctggatg gtttcgggtt tgaaatttta ggagggagaa ttgttatgga
    15001 cggcgaatat gatacccgca atattgaaaa gccaaatttc gatttcgttt tggatattaa
    15061 tcagctatcc ataaaggaat ccttcaatac ctttaacact gtccagacat ttgccccggt
    15121 tgccaaaaac atgacgggca attattcaac taagttcact atctcaggtg accttggaca
    15181 agacatgatg ccactcatga attcgatatc aggctccggc ttgattaaga tcgcgcaggc
    15241 agcattaaaa gattctcagg ttatctcggg tattaccagt cttaccaaat tgagtaattc
    15301 agatgaggta agtatcaagg atttggcact ttcagtttcc attaaagatg gaaggcttag
    15361 tgtaaaacca tttgatatta aattgggaaa ttacaaaacc aatatttcag gcagcaccgg
    15421 gcttgagggt gatttggatt atgtgctgga tatggatatt cccgcaggtg aaattggaag
    15481 ccagctgaac aatactcttg cttcacttac aggatcagca cccaaagatt ccgatgtcat
    15541 aaaactgaag attggattaa agggaactta tgataaacca agaccggcat taattggttc
    15601 cggcgcgcag gcacaagttc aggagaaagt tacgcaggct gcaacagatg aagccaaaaa
    15661 gctggtgagc aaagaactca ccagcgaccc taatgcacaa aaagcagtgg attcattggc
    15721 aaaaatagtg gaggatccgg aagcaattaa aaaaatggcc gaagccgccc aaaagaaaat
    15781 tgaagatagc attaagacag tacttgctgc caaacagaaa gctattgaag acagcattaa
    15841 gaaagctgcc gcggaagagg ctcagaagaa aataaaggat ttatttaaga agaaaggtgg
    15901 caattaaaga tcgcttcttt caccaaccgt taaaacttct atgtcaaaaa taagattggc
    15961 attcggcggg attgagcccc ggccggccgg cccgtatccg agttgagatg ggatatgaac
    16021 tttcaaacgg tcgccttctt tcatatacga taaggcttcc tcaaatcccg ggatgtgaga
    16081 agtaactcca tgaacaaaga catattctct tccgtcatcc cggcttgacc aaataagcgt
    16141 gccatcttcc agggtgagga tgcaatgagt tgtgatcttt ttgccattct caacttcagg
    16201 tcctgaccca tttttaatca ttgtatactt taagccggaa tcggtagtaa tttcatcagt
    16261 tccggaatcg gaagcatctg caatctcttg cctggaggat gatgtacaag aaacaatcag
    16321 ggaaacggta aggacaagta aaaagaattt ttgcataatt attttttttc caatactaaa
    16381 acaattttgt taaaatcggc agctgcatta atcacttttc taaattcttc aaaatcttcc
    16441 accggacaac gcagctcatt ataatattct tcacaaacca gttttaaaac atttccttca
    16501 atggtgtagt tggaggtaaa gccaaaaatt gccttctcat ctttgagatg cagtacctcc
    16561 atgtttatat catccgggtt ctttaccaca tagccgtctg gaatggtgaa attaacatgc
    16621 ctgatatatt tgcggagata atcattttcc attggcatta ccctttttct tacctgatac
    16681 atttccacct gcggcccgat tatttctcca attttgaaca aaatttttga accggccttc
    16741 tcaacaattg ttgaagaagt gaattttgcc ttgataataa aggggaaatc caggggtgat
    16801 aaatttgctt ctccattttc aacttgaata gattgaaaca cagcatcttc ggccgcactt
    16861 cgaacaaagt cttcaagaat tgttctgctg tcattctcgg gaataaagga ataataaggt
    16921 tgaatggata acgcattgta gccacccatt gttcgcttaa agtcaatttg tactgaagac
    16981 attccggttt caaacttcat atcaatttcc attttgtcga aactctggtc ggcacctatg
    17041 gcagggatgt attcaacccg tccttccgcg gattcaaaat taccaagaga aaacgtttta
    17101 ataaacaacc cattattgtc agtcaaaaag aaaggaacca tcccgtacct gtattcatac
    17161 ttatccggac tcaggaattt tttggttccg ggaaagtaga aaagatattc ggagaggtta
    17221 ttccaatttt cgaactctga gtcaaaacct gtttcatccc tcccattggt taaaactatt
    17281 tccgagtcaa tctcaagttg cttgaagatg gcagcaaata atcttacaat accgttagta
    17341 ttcgtataat tacgagctat tatcatggct aattcatcaa tatctgaaag cgcgccttcc
    17401 tgaatactga tatgagtttt tacataattt tcaatttgtc tgattttact ttcaatatca
    17461 acttctttaa attcaataag actaataaat ttttgtacgg cttctttatc aagatccgag
    17521 acacccccgt aaatgacttc gtaaatacgc tgagtcgcat cattccatgt aagtatctca
    17581 cccggaccct gatcaaagtt gaaagcaagt ttatactcta ctctcatccg gcttgcagtg
    17641 tatgcggaat actcttcatt ttccaattca ggaatatatc tcacggcggc agaataatac
    17701 cgcttctgat cctttacttc ctcatttacc tgaggatacc cgttataccc tttaaattca
    17761 aatttcagat gctcgggaca gatcagaaag aattcggaat tcaggatggg aattgattct
    17821 tgcacgaatt cgcttccgaa atatcttttt ccaaccttag cagtgtattt atactctacc
    17881 tcgctcccgg cttcaagtcc ttcaacagca aaaatccgga aagcatttcc agtctcatca
    17941 tcttttattt ccttgatatt atcgctgttc aggtcctgga taccgccgga aggacttata
    18001 gatcgcgcct gaatggaaat caattcaacc gcattataca tgggtacata aatcctgtta
    18061 tgccgctgga ttccttcatc attattaacc ctgattattt tatgcacggt agagtaaatg
    18121 tataaatcgt cctcctcctc aaaatattca ttcagaagat aatcttttaa ataaagcgac
    18181 ggtaatttct catgaccagg ggtgatagaa atcctttctc tcccttcatg ccattcggta
    18241 ttcttatatt cctgagaaaa agacagagca ggaagcaaag tccatactga tattatgatt
    18301 ttcttgaaca tttatttctt ttttaggatt aatgcgtctg tataggcttc attaatttta
    18361 ttaataacgt cattccaagc cgggaatttc tctaatgcta gaattaaata ttctacaaac
    18421 acggtcttgc gaagaatcac tttacttccc aataattcat aattaattga atatcctgca
    18481 acttcattca tgaactccga attccgggga agatgttcaa tttcgtaccc gtttggaatt
    18541 tccagtgtcg taatgtaggt gtgctcatac ttgtattccg tctcaatggg atattttctc
    18601 ctctcaatat cgattaattg attaaaaaat ggtttgctaa gattcaaatt caaataaatc
    18661 tcatcgccaa tctgcttata gtaatcctga atgttgaaag aatatgttat ttctaaaggc
    18721 aaatctttat ttgtaaggtt ggtcagatta taattttcca gcaggaattt gttgcttcca
    18781 cgggtcagtt tagctttgat caattcatcc aacttagttc cggaagcaaa agttattctg
    18841 tatgtatcaa aaatcttttc ataaccgccc tcggaaagta taccatttcc aataacccga
    18901 ccgtttgata tttttatgat acttgaatct cgcttaaaat ttactttgcg gtcaattaca
    18961 ggcactgttt ccagccggta actgtcaggt ccggtctgca cgagggcttg ctttccctgg
    19021 atcatggaag aagggagtcc atactgggta tatttacttg ttgcatcaag aaattgagga
    19081 tccccatctt tgtaataagt ggctatcatg tgattatcaa caatcggcgt tggaacttcc
    19141 tcataagaat aggggatatc ccgcgagcct atccaggtga agtaggaatt gatgcctgca
    19201 attttaagca tgctgcgggt gatgcaagcc atgtccttac aatcgccata ccggttggaa
    19261 aaaatgtaat ttgcattgtg aggtataaat ccccgcatgc cttcctcgaa tgcgatgtat
    19321 tttatgttat cctgaaccca ataaaaaata gcctgtactt tttcctcctc cgtggcgagg
    19381 tcttgcgtga gtccttcaac tgtttttctt aaatcaggac tttcttcact atccagatga
    19441 gaggtaaaag tagaatacca atcaaacaaa tttttcactt ctcctaaaat cacgtgctct
    19501 tcagttcctt ttttaacttt tgaaatataa taa
//
LOCUS       DASWHX010000032         6935 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_2241109, whole genome shotgun sequence.
ACCESSION   DASWHX010000032 DASWHX010000000
VERSION     DASWHX010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 6935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6935
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2241109"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..585
                     /locus_tag="VGA21_01935"
     CDS             <1..585
                     /locus_tag="VGA21_01935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEX9649296.1"
                     /translation="GLTAFYMFRLYFIVFWGSEKSYEKQPLESPNIMLIPMIFLALGS
                     ALAGFVPFGKFISSDGIALEPHINWTVALISVGITFVGIITAALFYRKKSSTPDRIKT
                     ALGALYLGASRKFYIDEVYLFITRRIIFNMISKPIAWFDRKIVDGFMNAIGNSTRSIS
                     EDIKELQSGQLQKYAFIFLSGVLIILLTFIYLNQ"
     gene            586..2076
                     /locus_tag="VGA21_01940"
     CDS             586..2076
                     /locus_tag="VGA21_01940"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEX9649297.1"
                     /translation="MNILTIIILVPALTTLLVGLSKSRIQVRLIAAVGMGIQLILSVY
                     LLFAYLTERKAGNTAQVLFSKNILWFESLNINYAIGVDGISVAMILLTSIVIFAGIFA
                     SWEVENLYKEFFVSLILLATGVFGFFVSLDLFLMFLFFELAVIPMYLLIGIWGSGPRE
                     YAAMKLTLMLMTGSALLLVGILGIYFNSNIDGGHSFNIIELSNQSLPLGVQRVFFPFV
                     FIGFGVIGALFPFHTWSPDGHSSAPTAVSMLHAGVLMKLGGYGCLKVAIFLLPDAAQE
                     MSWIFLILTAISVIYGALGTIMQTDLKYINAYSSVSHCGLVLFALLMLNKTATNGAVL
                     QMVSHGLMTALFFALIGMLYSRTHTRIVQEMGGLMKVLPFLSVCYVIAGLASLGLPGL
                     SGFVAEMTIFVGAFQHPDAFHRIITIIAVSSIVVTAVYILRVVGTLLMGEVKNDSYNE
                     FPPAKWYEKLATTSLIIGILIVGLIPFWLSNMISDSLGIIVSRLIP"
     gene            2085..3500
                     /locus_tag="VGA21_01945"
     CDS             2085..3500
                     /locus_tag="VGA21_01945"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009194939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HEX9649298.1"
                     /translation="MELSDFLIMRHEILLITGVLLILIVDLSIPDNKKKKIPVIASFV
                     MGIITLFGFYPVDTGSLFGNMYVTDMVRLTMKNILNLGVFLIFLQSSQWLCNKENDKK
                     IAEFYLLILSSLIGVNYLISSGHFIMFYLGLELTTIPIAALASFHQYQSKSAEAGIKL
                     ILSSAFSSAILLFGISILYASLGSLYFNQMHLGSDIINVMGFIFFFSGIAFKISIVPF
                     HLWTADVYEGSPTSVTAYLSVVSKGSAIFILLLTLGWIFRPLSQVSQDILYAVAIITM
                     TIGNLFAIRQQNIKRFLAFSSIAQAGFILLGLVGGTSLGITSVIYFILIYVFSNLGAF
                     GVVIAVEQSSGKVNINDYDGLYRTNPRLSLVMMLALFSLAGIPPVAGFFGKFFLFTAA
                     AESGFYTLLLIAVLNAIISLYYYLLVVKAMFINKSDSPVAGFKSDFSTRLSLMICVVG
                     IIVTGFYSPIFEFIKELSLGI"
     gene            3503..3853
                     /locus_tag="VGA21_01950"
     CDS             3503..3853
                     /locus_tag="VGA21_01950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649299.1"
                     /translation="MSLNKFQHTIIDIDGIDCRVVEADLTKERAEFLQKLLLLNSVES
                     KIQEKPAMKEGEVTTYTIATPDITFNPIVKVYNRELRTFEGHRVTPDYWNQKTKDTEP
                     NYWDLQKKSWIKKS"
     gene            complement(3865..6306)
                     /locus_tag="VGA21_01955"
     CDS             complement(3865..6306)
                     /locus_tag="VGA21_01955"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9649300.1"
                     /translation="MIKNYLKITLRSLWRQRFFTFVNAFGLSIALAFCTLIYLFILDE
                     SRFDKFHSNKKDIFRMNEVSYLYENNSSFYVGGAQAESVRKSAWLPAALGPTLKDEMA
                     EVIRATRFNEGSVVLKHNEKIFKEDMAFVDADFFKMFSFKVLSGNPDHFFDLNEEIVL
                     TESIARKYFGNDDPVNQTIIVRIGSEEKSLTITGVLADPPSYSSLEFGVLIPSPNRSY
                     YERNMQNWNSFNSPTFVQLSPNVISENFEANLRVFTKKYFQGQIEYWRERQKIPEGEP
                     DFELGFTNLADIHLTTDVNWNKVSDPKYAYILGGIAVLILIIACINYVSLSLSRSIKR
                     RLEVGIRKVSGAQKGQLISQFLFESMLQSFVAMVIAVALIHTFLPWFNDYTLKGISLG
                     LKEWATPGIFIFILSIATGILAGSYPSFFLSKQKPISVLKKEVAKVRSRFSTFLVGLQ
                     FFLSGGLIICSVIMFKQMNFITQKDLGYNEEQVVVIPTYTGWTDEGEETVKRFRHRFA
                     GDPDIIGVSGTSASFNRGWSRNGYKVNGEQKAAFVYRVDEEYIKLLDIELIAGRNFDP
                     AIPSDKNSLIINEALAKDMGYDDPLAERLDWHEDSVGYQIIGVVKDYNFLSLEHEIDP
                     MFIMINPEDDKISTMLVKLAPGKIGEGLSKLQTGWNELVSDKPFDYSFLDEDVARQYE
                     SYSKWTKITGLSTFFAILIASLGLFGLAGINASNRTKEIGIRKVLGAGVSHILFLMTR
                     HYVVLSLIAFAISAPLSWYIMGKWLSDFEYSISLSWELFVIAMAGGLLITLLTVSYHS
                     IKSTIVNPADTLRYE"
BASE COUNT         1954 a         1342 c         1342 g         2297 t
ORIGIN      
        1 gggttaacgg ctttctatat gttccgtctt tactttattg ttttctgggg aagtgagaag
       61 tcatatgaaa aacaacccct tgagtctccc aacatcatgt tgattccaat gatttttttg
      121 gcactgggtt ctgccttggc aggcttcgtg ccgtttggta agtttattag ttctgatggt
      181 attgctcttg agccgcatat aaattggaca gttgccctaa tatcagtcgg aattacgttt
      241 gtaggtataa ttacggctgc tttattttac cgaaagaaat catcaacccc ggatagaatt
      301 aagacagctc tgggcgcact ttatttaggg gcaagcagaa aattctacat agatgaagtt
      361 taccttttca taaccaggag aattattttc aatatgatat ccaaacctat tgcctggttt
      421 gacagaaaaa ttgttgatgg atttatgaat gcaattggca attcaacccg ttcaatttct
      481 gaagatataa aagaattgca atcaggccaa ctgcagaaat acgcgttcat attcttatct
      541 ggagttttaa taatactgct aacatttatt tatctgaatc agtaaatgaa tattttaacg
      601 attattattc ttgttcctgc tctaaccacc ttgctggtgg gattaagcaa atcacgcatt
      661 caagtcagat taattgcggc ggtgggcatg ggaattcaat taatactttc tgtttatctt
      721 ctgtttgcct acctgacaga gagaaaagca ggtaataccg ctcaggtatt attctctaaa
      781 aatattttat ggtttgaaag tctcaatatc aattatgcaa tcggtgtcga tggaatttca
      841 gtggccatga ttctccttac ctcaatcgtt attttcgcgg gaatttttgc cagctgggaa
      901 gttgaaaatt tatataagga attttttgtt tcgcttattc tgcttgcaac cggcgtattc
      961 ggattttttg tatccctgga tttgtttttg atgttcctct tttttgagtt ggccgtaata
     1021 cccatgtacc tgttaatagg aatatgggga agtggcccca gggaatacgc tgcaatgaag
     1081 ttaactctta tgctaatgac cggttctgct ttgttgttag tgggcattct cggaatttat
     1141 ttcaattcta atattgatgg cggacactct ttcaatatta ttgaattgtc aaaccaaagt
     1201 cttcccctgg gagttcaaag ggttttcttc ccatttgttt ttattggttt cggtgtcata
     1261 ggcgccttgt ttccattcca tacctggtct cctgatggtc atagttccgc acccacagcg
     1321 gttagtatgt tacatgccgg agtgctaatg aaacttggtg gttacggctg tttgaaagtt
     1381 gccatttttc ttttgccgga tgcagcccag gaaatgagct ggattttcct gattctgacc
     1441 gctatctcgg tgatttatgg agccctggga acaattatgc aaacagatct aaaatatatt
     1501 aacgcctact cctctgttag ccattgcgga cttgttttgt ttgcattatt aatgctgaat
     1561 aagactgcta caaatggagc agtcctgcaa atggtttccc atggattgat gaccgctctt
     1621 ttctttgctt tgattggaat gctgtattca cgaacccata caagaattgt tcaggaaatg
     1681 gggggtctga tgaaagtcct gcctttcctt tccgtttgct atgtaattgc gggccttgct
     1741 tcacttgggc ttcccggctt aagtggtttt gtcgctgaga tgactatttt tgtcggagct
     1801 tttcagcacc cggatgcctt tcaccgtatt attacgataa tcgcagtttc atctattgtt
     1861 gtcaccgctg tttatattct cagggttgtt ggcactttat tgatgggaga agtgaaaaat
     1921 gatagttata acgaatttcc acctgccaaa tggtacgaaa aactggcaac aacaagtttg
     1981 ataattggga ttctcattgt cggcttaatc cctttttggc tgtctaacat gatcagcgat
     2041 agtttgggaa taattgtttc acgattaata ccttaagtta taaaatggaa ctcagcgatt
     2101 tcctgattat gcgccacgaa atactcctga taacaggtgt gcttctaatc ctgattgttg
     2161 atttatctat tcctgataac aagaaaaaga aaataccggt tattgcttca tttgttatgg
     2221 gcataattac tctttttgga ttctatcctg ttgataccgg aagcctgttc ggaaacatgt
     2281 atgtaactga catggttcgg ttaaccatga aaaatatttt aaacctgggt gtttttttga
     2341 tcttccttca gtcatcgcaa tggttgtgca ataaagaaaa tgataaaaag attgctgaat
     2401 tttatttgct gatcctgtca agtttgatag gagttaatta tctgatttct tcagggcatt
     2461 ttattatgtt ttatttggga ttagaattaa ccactattcc aattgctgca cttgcatctt
     2521 ttcaccaata tcaaagtaaa tctgccgagg ccggcattaa attgatcctt tcttcagcct
     2581 tttcttctgc tatcctgtta tttgggattt caattcttta tgcgtcttta ggttcactgt
     2641 actttaatca aatgcatctg ggaagtgaca taatcaatgt aatgggattc attttcttct
     2701 tttcgggcat tgctttcaaa attagtattg tgccatttca cctctggact gccgatgtat
     2761 atgaaggaag tccaacaagc gtcaccgcgt atttgtctgt tgtttccaaa ggatcagcaa
     2821 ttttcattct attgcttact ctgggctgga tattccgccc gcttagtcaa gtctcgcaag
     2881 acatcttata tgctgtcgca attattacta tgacaatagg aaatctattt gccatcaggc
     2941 aacaaaatat caaaaggttc ttagcctttt cctccatcgc gcaagccgga tttatcctgc
     3001 tgggattggt gggtggaact tcgctgggaa ttacctcggt aatatatttc attttgatat
     3061 atgtgttttc aaaccttgga gcttttgggg tagtaattgc cgtagaacaa tcatcaggca
     3121 aagttaatat taatgattat gatggactgt accgtacaaa tcctcgttta agtttggtaa
     3181 tgatgctggc tcttttttca ttagccggca ttcctccagt tgccgggttc tttggtaaat
     3241 tctttttgtt tactgccgcc gcggaaagcg gattctatac attactgcta atcgctgttc
     3301 ttaatgccat tatctctctg tactattact tattggtagt caaagccatg tttataaata
     3361 aaagtgatag tccggtcgct ggttttaaat cagattttag cactcgtctt tcattaatga
     3421 tttgcgtagt tggaattata gtcaccggat tttatagccc gatttttgag ttcataaaag
     3481 aattaagttt gggaatttag atatgtcgct gaataaattt caacatacca taattgacat
     3541 agatggaata gactgcagag tagttgaagc ggatcttacc aaagaacgtg cggaatttct
     3601 gcaaaaactt ttattactta attcagttga atctaaaatc caggagaaac ctgccatgaa
     3661 agaaggcgaa gttactacat acaccattgc aacacctgat atcactttta atcctattgt
     3721 caaagtctac aacagggagt tacgtacatt cgaggggcat agggtaacac cggattattg
     3781 gaatcagaag actaaggata ccgaaccaaa ctactgggac cttcaaaaga aatcctggat
     3841 taagaaatca tagtaaaatt tttattactc atatcgtaaa gtatccgctg gatttacaat
     3901 cgttgacttg atagaatgat agctcacggt gagcaaggta attaataatc cccccgccat
     3961 agcgattaca aacaattccc agctcagaga gatagaatat tcaaagtcgc ttaaccattt
     4021 tcccatgatg taccaggaaa ggggagccga tattgcaaat gctattaaac tgagtactac
     4081 ataatgcctg gtcataagga agaggatatg gcttacaccg gcaccaagaa cttttctgat
     4141 tccgatttct ttggttctgt tagaggcatt gattccggca agtccaaaca ggccaaggga
     4201 ggctataaga attgcgaaga acgttgagag tccggtaatc ttcgtccact ttgaatagga
     4261 ttcatattgc cttgctacat cttcgtcaag aaatgagtaa tcaaaaggct tatcggaaac
     4321 aagttcattc cagcctgtct gaagctttga taatccttcg ccaattttcc ctggagctaa
     4381 ctttacaagc atggtagaaa ttttatcatc ctcagggttt atcatgataa acataggatc
     4441 aatttcatgc tccagggaaa ggaaattata gtcttttaca actccaataa tctgataccc
     4501 aacggaatct tcgtgccaat ccaatctctc agccagagga tcatcatacc ccatatcttt
     4561 ggccagggct tcatttatta tcagagaatt cttgtccgaa ggaatagcgg gatcaaaatt
     4621 tctccctgcg atcaattcaa tatccagcag tttgatgtat tcttcatcaa cccggtatac
     4681 aaacgctgct ttctgttcgc cattgacttt ataaccattc cttgaccacc ctcggttaaa
     4741 tgacgcactt gttccggaaa ctccaataat gtccggatct cctgcaaatc gatgtctaaa
     4801 tcgcttaacc gtttcctctc cctcgtctgt ccatcctgta tatgtaggta ttaccaccac
     4861 ttgctcttca ttatacccca agtctttttg ggtaataaaa ttcatttgtt tgaacataat
     4921 tacagaacat attattaatc caccagaaag gaaaaattgc agacctacta aaaatgtcga
     4981 aaaccttgat ctgacctttg ctacttcttt ttttagaaca gatatcggct tttgttttga
     5041 aaggaagaaa gaagggtaac tgccggcaag tattcctgtg gcgatactta atatgaaaat
     5101 aaatattccc ggagttgccc attccttcag tcctagtgaa attcctttga gcgtatagtc
     5161 gttaaaccat ggcagaaaag tgtgaatcag ggccacggca ataaccatgg caacaaatga
     5221 ttgaagcatt gattcgaaga ggaactggct aataagctgc cctttctgag ctcccgagac
     5281 cttcctgatt cccacctcca gccttcgctt aatagatctg gaaagggaaa gggacacata
     5341 atttatgcaa gcaataatta aaattagcac ggcaattcca cccaggatat acgcgtactt
     5401 aggatcgctg actttattcc aattcacatc ggtcgtcagg tgaatatcag caagatttgt
     5461 gaaacccaat tcaaaatccg gctccccttc cggaattttt tgcctttcgc gccagtactc
     5521 aatttgaccc tgaaaatatt ttttagtgaa aacccgcagg ttggcttcaa aattctctga
     5581 gataacattg ggcgaaagct gaacaaacgt tggagaatta aaagaattcc aattctgcat
     5641 atttctttca taataagacc tgtttgggga aggaatcaac acaccgaatt caaggctgga
     5701 atatgagggc ggatctgcta aaactcccgt aattgttaag gacttttctt cacttcctat
     5761 cctgacaatt atggtttgat tcaccggatc atcgtttccg aaatattttc tggcaatgct
     5821 ttcggttagt acaatttctt cattcaaatc aaaaaaatga tctggattcc ctgacaaaac
     5881 cttaaaggaa aacattttga aaaagtctgc atctacaaaa gccatatctt ccttgaaaat
     5941 tttttcattg tgtttcaaaa ccacactgcc ttcgttaaat ctggttgccc tgattacttc
     6001 cgccatttca tcttttagtg ttgggcccaa cgccgccgga agccaggctg acttccgtac
     6061 actttctgcc tgagctccgc ctacataaaa ggaagaattg ttttcataca gataactaac
     6121 ctcattcatt ctgaagatat cttttttgtt agaatgaaat ttatcaaatc gggattcatc
     6181 cagaataaac aaatagataa gagtacaaaa ggccaatgcg atgctaagtc caaatgcatt
     6241 cacaaacgtg aaaaatcgct gacgccataa gcttcgaagg gttattttca ggtagttctt
     6301 aatcatggtt tttaaggtag gtatatgaag caatgatgat aaaacattcc aaaaagtttt
     6361 attttcaaca ttaaattatt ttattcttaa ttaaatctta aacatatttc gatgagttgt
     6421 tgattcgccg gattgaatta actcaaaatc tggttaaatt aactttaatc attatctgat
     6481 tttttggacc actatggagc cggaaatttt ggggcagtgg ccaatgagga ttgatgtggt
     6541 ttaccaatgc tgtagcctta aggaaatatt ttgtacttct ttaaccagtg cctccttctt
     6601 tattgttgtc gggtttctgg ctaatggcag gaaggcatat gaaaaaaaga atgaattgac
     6661 atgcagtttt tcaatcttgc aagattttgg aatattgaaa atatttagtt tcaaaatata
     6721 agaaaatggc ttataaatcg ttaattcatt ggtatatata actttggcat aaggtttggt
     6781 ttatatctat taggctaaaa ataccgccta tgtgtggttt gtgtgtgggg atgattagga
     6841 cagctccggg aagctattcc cggagctctt ttttgatttc aagcccattt atcattagtt
     6901 gtgcccccca aatagtcttg agcatctcct taggc
//
LOCUS       DASWHX010000033         7836 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_23453, whole genome shotgun sequence.
ACCESSION   DASWHX010000033 DASWHX010000000
VERSION     DASWHX010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 7836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7836
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23453"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(566..1414)
                     /locus_tag="VGA21_01960"
     CDS             complement(566..1414)
                     /locus_tag="VGA21_01960"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HEX9649301.1"
                     /translation="MHKDRWLNDFEVNVTSQNGEDGIITKIFEFIPYPDKWCVEFGAW
                     DGKFLSNCYNLLANKSWKGVMIEAQKKKYKELIRTFSGNKEVYCINKFVNFEGPGSLD
                     EILNNTPIPQDFDLLSIDIDGNDYHVWNSLHTYRPKVVIIEYNHSIPSDIEFIQKKNN
                     SVNHGSSLLSLTRLAKTKKYELVCTTLCNAIYVDEKYFKLFNIEDNSINVLFKPQYET
                     PRIFQLYDGTLMLTSEFRLFWKSPMVVKPKDLQVIPKYLRFKNDSGVSKIRRLLFRIF
                     LMLKGQ"
     gene            1668..1811
                     /locus_tag="VGA21_01965"
     CDS             1668..1811
                     /locus_tag="VGA21_01965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649302.1"
                     /translation="MKDLAPDIFRKRMLIEGYYTINIYKEATNTIHNEVGLHFVTYTV
                     VDP"
     gene            2063..5389
                     /locus_tag="VGA21_01970"
     CDS             2063..5389
                     /locus_tag="VGA21_01970"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing tetratricopeptide repeat
                     protein"
                     /protein_id="HEX9649303.1"
                     /translation="MFLGRIFVGILIGISLFLSGNVFAQKPDTVLARQLFELGNRQRA
                     AGNYDSAFLNWSWAGDIYKQNSAWTHYATILYSIGKTYSEIADYDQGLKYLEESRDLS
                     IEKIGEVSHEAAWAYYGLAWYYGAVGEYDLAYESQKKSLSIRLKLFGENNKSVVDSYN
                     NLAFIYRMQGKYDSAYNVYAKALQITLKVLGDENTITASTYFGLGWVSAALGDMRKSL
                     DLHKKALEIRLKILDPTSSAIATSYNNIGFCYNQLGDYGQASRFYQKALDLRIKRFGE
                     NHPNVASSYLNIAQLLALNGDNDRAILYQSKGIRIWSELYGIEDQEMARNYNYLGSFY
                     FNKGEYRTALKYHLEALQVAEKKEQHPFLPLTYNYLGKVYAKLGDKEKQFEYLQKAQK
                     GELEIFGPIHQNTANSYAELADFYKSIGNAEKEFEFLQKCLSARLQLFGEEHPDVAIT
                     YSRLGDFYRRKNNFDSAISNCQKGLISAVPGFNNLDWKVNPSLSGYYSRLVLLKILSD
                     KVTILAYFAQKLPAQDLLRQAFDTNYKAVQLLDTLRHGNLSEGSKQEIIANSLSTYEL
                     GIDLALSLFNNSGDKKYLKNAFSMMEKSKNFLLLVSLKNSRAQHFGEVPDSLIQAESN
                     LKMDVSYWEQRVLAARKKGENSKALEYESRLVNSKFLYQSFVNDLRRSHPQYFSLKYE
                     NAPPEIEEIQNEILSKTGALIEYFAGEDNYFAFIITKDNVALTKIPNAGNLNVIAYNF
                     RQSISDFDFISNNSSVADSMYCHSANFLFEQLVGMPLSAMNEMPAKIIIIPDGILNHL
                     NFDALLTSMPVDDTHLNYGSLKYAINDFQISLANSATILTSTGFSRKKMKPANIFAGF
                     APDYSGIAESDTLDNQFMAVLVRSGNLQLPGASEEVNLIANLVKGNAFTGAKATEKLF
                     KEVAGNYKILHLAMHGLIDDLEPLNSELVFTPVQDSINDGYLNLSEVYNLRLHSELVV
                     LSACNTGVGKIQKGEGNMSLSRAFRYAGSEGIIMSLWKVPDEATSEIMVDFYKNILNE
                     KTKDQSLREAKLAFLKKYADDPLQSHPFFWAGFVPIGIMKPVELESNYFLVISFILGT
                     MLLVLIIYSFRKRMSNRSVG"
     gene            complement(5386..6141)
                     /locus_tag="VGA21_01975"
     CDS             complement(5386..6141)
                     /locus_tag="VGA21_01975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649304.1"
                     /translation="MDSKNIHISEELLRDYSLEIISNAARARIDNHLQECDQCSAVLE
                     GLKLAGEEVLHGEDFMADLKSRNLELIQQNKYAAANPNRLGSLLKVAAVILILVAASV
                     AILLQRSPAAQDLVSQNLTQPYAAPPLERGENSLNENWQKGIEAYRSRNFESAVSFIQ
                     ESYNQEANDEKEFYLGLSLLYQQNSEPEKAAVHFENLINTRYSEQALWYKILAYYLSG
                     EVDSCKTSLEKIVSSKVHYKKKAAERLLKKLNP"
     gene            complement(6147..6716)
                     /locus_tag="VGA21_01980"
     CDS             complement(6147..6716)
                     /locus_tag="VGA21_01980"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX9649305.1"
                     /translation="MSSEIKGSFNQEALVKRLWESYVKENDKNAFGKLYSIYHNRLTL
                     YCLGKLRNRELAENASSETLIKLLQVEKPEEISDFESWLFKVAKNTCLTLLSTANRRQ
                     EILQTRHKSISDSGKPEIEKKMDADDLRELIHKTLDETNYEIWELHQMGYDNEEIGEK
                     LNMNIKTVANRKAEARRQLREKIKNTQNL"
     gene            6849..>7836
                     /locus_tag="VGA21_01985"
     CDS             6849..>7836
                     /locus_tag="VGA21_01985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649306.1"
                     /translation="MKIITLVLLAGISISAYSQTTLIANNNPGAAPGTASNPNVFTGA
                     TALTDAFTAASAGDIIYIVPGGNNYGALIITKSITIFGIGLDPDKDGAALSYMTNVTI
                     RASDVRISGITFTDLTSILLDPAGSVISNIMIDKCLFLRLLRNTNGDAGNIIIQNCIG
                     GDGITGTGDQAIQLETSYSNVRIANNIFHGNGNTQGGTLDMINGADIEHNLFISSTSG
                     SAQRAFGDVTNCNIRNNIFYGIRAQASNTFTGNNIDNNITNFCSDNTIQGGNGNVLTN
                     NLEGVDPQLVNVPFSVRYNFSDDPSLIPGSPCEGAADDGTDIGLFGGGSPMDI"
BASE COUNT         2350 a         1553 c         1503 g         2430 t
ORIGIN      
        1 gagattttat ttaacaagtg aaaaggagtg gaaatttctc gttcattttg agggatgatg
       61 tcccctagcc cttgttggtg ttcttttcac caacaagcaa accgaagttt taatgacgtt
      121 acatttacaa tcacttatca cctctagcct tggttggtgt ttgcgctggg taaatgggat
      181 ggattcacca accaaattac agtttccggc cccgcaatcc agtcgacaac cgtaaaagta
      241 acaaagtgta atcctacttc attgtggata gtatatttat cccacatgat atcccgacca
      301 cttgtggttg gcaatggtaa actgactgga tattcggtgt tggttcaaac caattaatta
      361 gaatggatat ctctaacaac ctgccatgcg aaagaattat caattgagca gccctaagtt
      421 ttcaaaaatt ctctggttaa tattagccca tacggaaact tctttctcgc gactcgttgt
      481 tatattaaat tctcacgaag gatgcacgga agggttggct ttctgtggaa atctaaggtg
      541 aacgatcgtt taacacacgc aaaaattatt ggcctttcaa catcaggaag atccggaaca
      601 aaagtcttct tattttactt actccgctgt cattcttaaa cctaagatac ttcggtatca
      661 cttgcaggtc tttgggtttg acgaccatag ggcttttcca gaacaacctg aattcagaag
      721 ttaacattag tgttccatcg tataattgaa aaattcttgg tgtttcatat tgcggtttga
      781 ataagacatt gatactgttg tcttcaatat taaacaactt aaaatacttt tcatcaacat
      841 agatggcatt gcacaacgtt gtacacacaa gctcatattt tttcgtcttt gctaaccggg
      901 taagggataa caatgaagaa ccgtgattta cactattatt tttcttttga ataaattcaa
      961 tatccgaagg gatggagtga ttgtactcaa ttataaccac cttcggtcga taagtatgca
     1021 gggagttcca tacatgataa tcattcccat caatatcgat agataataga tcaaaatcct
     1081 ggggtatcgg agtgttgttc aagatttcgt caagacttcc gggaccttca aagttcacaa
     1141 atttgtttat gcagtatact tctttatttc ctgagaaggt tcttatcaat tctttgtatt
     1201 tctttttttg ggcctcaatc atcactccct tccatgattt gtttgcaagc agattgtagc
     1261 aattgctcag aaatttgcca tcccacgcac caaattccac gcaccatttg tccggataag
     1321 ggataaactc aaaaattttg gtgataatcc cgtcttcgcc attttgagag gttacattta
     1381 cctcaaagtc gtttaaccac cggtctttat gcatgttctt tcatttgggt ttttggcata
     1441 aggaggacat caattttttg gtctttagcc acgtaacggc aaatcatgaa ttcatgatca
     1501 taaaacggcg taaggaggca aaggtatgtg attcataacc agttccgcgg aaacaattat
     1561 tacatgaaac agtttctgcg aaattcccgt gtaaaattcg tttaaattgt aatacaaatt
     1621 aaagaagaac cgacaatttt agttcagtat aaataatttg gaaaatcatg aaagatctgg
     1681 cgcctgatat ttttagaaaa cgaatgctga ttgaaggtta ttataccatt aatatttata
     1741 aggaagcaac aaacactatc cacaatgaag taggattaca ctttgtcacg tacactgtag
     1801 ttgaccctta gccttggttg gtgtttgcgc gggtaagtgg gatggattca ccaaccaaat
     1861 tacgtttccg gcccggcaac ccagtcgaca accgtaaaag taacaaagta taatcctact
     1921 tcattgtgga tagtatattt atcccacatg aatatcccgc ccacttgtgg ttacagccct
     1981 aagttttcaa aaattctctg gttaataagg gtgtaatttg ctactttaag attaccatcc
     2041 aatctcatcc aatttataac ccatgttttt aggcagaatt ttcgtaggta tcttaatcgg
     2101 tatttccctg tttctttccg gaaatgtttt tgcgcagaag ccggatactg ttttagccag
     2161 acaattattt gagctcggaa atcggcagcg agctgccggc aattatgata gcgcatttct
     2221 gaattggagt tgggctggtg acatttacaa acaaaattcc gcatggacgc attatgcaac
     2281 tatactttat tcgattggca agacctatag cgagatagct gattatgatc agggtctgaa
     2341 ataccttgaa gaatccaggg acctttctat tgagaagata ggggaagtta gtcatgaagc
     2401 cgcctgggcg tattatggac ttgcctggta ttatggagct gttggtgaat atgatttagc
     2461 ctatgaaagc caaaaaaagt cattgagtat ccgattaaaa ttatttgggg agaataataa
     2521 atccgtcgtc gacagctata acaatcttgc atttatttac aggatgcagg gtaaatatga
     2581 ttcggcctat aatgtttatg caaaggcgct tcaaatcacc ttaaaagtgt taggggatga
     2641 aaatacaatt actgcttcca cttattttgg gttaggctgg gtaagtgccg ctctcggaga
     2701 catgaggaaa tcacttgacc ttcataaaaa agcattggaa ataagattaa agatccttga
     2761 tcccaccagc agcgctattg ccaccagtta taacaatatt ggtttttgtt acaatcagct
     2821 tggagattat ggtcaggcct ccaggtttta ccaaaaagca cttgatctga gaattaaacg
     2881 tttcggagaa aaccatccga atgttgcaag tagttatctt aatattgctc aacttctcgc
     2941 actgaatggt gataacgacc gggcaatttt atatcaatcc aaagggatcc gtatttggtc
     3001 agagctttat ggcatcgaag accaggaaat ggccaggaat tacaattatc tcggatcttt
     3061 ttattttaat aagggtgaat atcgaactgc cctcaaatat catttggaag cattgcaagt
     3121 agcagagaaa aaggaacaac atccctttct cccactcacc tataattatc ttggtaaggt
     3181 atacgccaaa ttgggagata aagaaaagca atttgaatat ttgcaaaaag cacagaaagg
     3241 agaacttgag atctttggtc ctatacatca gaatacagca aattcttatg cggaattggc
     3301 agatttctat aaatccattg gaaatgcaga gaaagaattt gaatttcttc aaaaatgtct
     3361 gagcgcacga cttcagttat tcggggaaga gcatccggat gttgccataa cgtattcaag
     3421 gctgggagat ttttatagga gaaaaaataa ttttgattca gctatttcca attgtcaaaa
     3481 aggattgata tctgcagtgc caggctttaa taatttggat tggaaggtaa atccttccct
     3541 gagtggatat tattccaggt tggtactgtt gaagatctta tctgacaaag taaccattct
     3601 tgcttacttt gctcaaaaat tacccgctca ggatttgctc aggcaggctt tcgatacaaa
     3661 ctataaagct gttcaacttc ttgacaccct tcgtcatgga aatttatccg aaggctcaaa
     3721 gcaggagatc attgctaatt ctttatcaac ttatgagctt gggatcgatc tggcccttag
     3781 cctctttaat aacagcggtg ataagaaata cctgaaaaat gctttttcca tgatggaaaa
     3841 aagtaaaaac tttcttctcc tcgtctccct taagaattcc cgggctcagc atttcggaga
     3901 agtccccgat tctttgatcc aggccgaatc gaatttgaaa atggatgtgt catattggga
     3961 acaacgggtt ttagctgccc gaaagaaagg agaaaattca aaagctttgg aatatgaatc
     4021 acggttggtg aatagtaaat ttttatatca atcatttgtc aatgatctga gaagatcgca
     4081 tcctcaatat tttagcctta agtacgaaaa cgcccctccc gaaattgaag aaattcaaaa
     4141 tgagatcctt tccaagaccg gtgccctgat tgaatatttt gccggagaag ataattattt
     4201 tgctttcata ataacaaaag acaatgttgc cctcacaaaa atccccaatg cgggtaacct
     4261 taacgttatc gcctataatt tcaggcaaag catttccgat tttgatttca tttcaaataa
     4321 ttcctctgtt gccgatagta tgtattgtca ttcagcaaat tttctttttg aacaattagt
     4381 agggatgcca cttagcgcca tgaatgagat gcctgcaaaa attattatta taccggatgg
     4441 cattttaaac caccttaatt ttgatgcttt attaacctcc atgcctgttg atgacacaca
     4501 tctaaattat ggttcactca agtacgccat caatgatttt caaataagcc tggctaactc
     4561 ggcaacaatt ttgaccagca caggtttctc ccggaaaaaa atgaaacccg ccaatatctt
     4621 tgcgggtttt gcccccgatt attcaggcat tgccgagtca gacactcttg ataaccaatt
     4681 tatggctgtg ctggtgaggt ccgggaattt gcagttgcca ggtgcatccg aagaagttaa
     4741 cctgatcgcc aatcttgtta aaggaaatgc atttacagga gccaaagcca cagagaaatt
     4801 attcaaggaa gtagctggaa attataaaat tttacatctc gccatgcacg ggttgatcga
     4861 tgatttggag ccattaaatt cagaattggt ttttactccc gtgcaggatt ccattaatga
     4921 tggctacctg aatctttcag aagtatacaa tctaagactt cattcagaat tagtggtgtt
     4981 gagcgcctgc aatactggtg taggtaaaat acaaaaggga gaaggaaaca tgagtctttc
     5041 aagggctttt agatatgcgg gaagcgaagg aataattatg agtctttgga aagttcccga
     5101 cgaggcaacc tccgaaatta tggtagattt ctataaaaat attctaaatg aaaaaacaaa
     5161 agaccagtcc ctcagagaag caaaattagc cttcctgaaa aagtacgcag atgatccttt
     5221 gcaaagccat cccttcttct gggcaggatt cgttccaata ggaattatga aaccggtgga
     5281 attggagtca aattactttt tggtgatctc gttcatttta gggacaatgc ttctggttct
     5341 gattatttat tctttcagga aaagaatgtc aaatcggagc gtgggttaag ggtttaactt
     5401 cttcaataat ctttcagccg cttttttctt ataatgcact ttactggata cgatcttttc
     5461 caaacttgtt ttgcatgaat caacttcacc cgataaataa taagccaaaa tcttatacca
     5521 caaggcctgt tctgaatacc ttgtgttaat taaattttca aaatgaaccg ctgctttttc
     5581 gggttcacta ttttgctggt aaagaaggct taaccccaga taaaattctt tttcatcatt
     5641 cgcctcctga ttataacttt cctgtatgaa agaaactgcg ctttcgaaat ttctgcttct
     5701 gtaagcctca atccctttct gccaattctc atttaatgaa ttttcacctc tctccaaagg
     5761 aggcgctgca taaggttgag tcagattctg cgaaacaaga tcctgtgcgg caggagacct
     5821 ttgcagcagg atagcaactg atgccgcaac caggattaga ataacggcag ccacttttag
     5881 caaagatcct aatcgatttg ggtttgctgc ggcatactta ttctgttgaa ttaattcgag
     5941 atttctgctt ttaaggtctg ccataaaatc ttccccatgc agaacctctt cccctgccag
     6001 cttcagccct tccaaaacag cggaacattg atcacattcc tgcaaatgat tatcgattct
     6061 cgctctggct gcattactta ttatttccaa agaataatcc cgcaatagtt cttctgatat
     6121 gtgtatattt ttggaatcca tttcaatcaa aggttctgtg tatttttgat tttttccctt
     6181 agctgtcttc ttgcctcagc ttttctgtta gccacagttt taatattcat atttaatttc
     6241 tctcctatct cctcattatc ataacccatt tgatgtaatt cccagatctc ataattcgtc
     6301 tcatctaaag ttttatgaat taattccctt agatcatctg catccatctt cttttcaatt
     6361 tcaggcttgc cagaatccga aattgatttg tgccttgttt gcaatatttc ctgccttctg
     6421 ttggcagttg ataagagggt aaggcacgta tttttcgcga ccttgaaaag ccagctttca
     6481 aaatctgaaa tttcttcagg cttttcaacc tgcaaaagtt ttattaaggt ctccgatgac
     6541 gcgttttcgg ccaattcacg attccttaat ttaccaaggc agtaaagtgt tagcctgttg
     6601 tgatagatcg aatacaattt tccaaaagca tttttgtcat tctctttaac ataagattcc
     6661 cataagcgtt tcactaacgc ctcctgattg aaagaacctt ttatttcaga actcatctat
     6721 acgactattg aaactaacca aattcccggt aagatgctta aaataaagtc gtggcaaaaa
     6781 agaaaccggg aaataaagga gtttgggtag tcttattaca ttaagaacta taaccaaaag
     6841 atacgactat gaaaatcatt acactggttt tgctggcggg aatatccatt tcggcctatt
     6901 cccaaactac cttaattgca aataataatc cgggggcagc tcccggtaca gcttcgaacc
     6961 caaacgtttt taccggtgca actgcactga cagatgcatt taccgctgcc agtgccggag
     7021 atattattta tatagttccc ggtggcaaca attacggagc tttgatcatt accaagtcca
     7081 ttacaatttt cgggattggc ttagacccgg ataaagatgg ggccgctctc tcctacatga
     7141 caaacgttac catcagggca tcagatgtta gaatttcggg aataactttt acagatttaa
     7201 cctccatcct ccttgatccg gctggttcag tgatctccaa tataatgata gataaatgtt
     7261 tatttttaag attgctcagg aacaccaatg gcgacgccgg aaacataatt atccaaaatt
     7321 gtattggagg tgatggtata acaggtacag gcgatcaggc aattcaactt gaaacgtcct
     7381 attccaacgt gagaattgca aataacattt tccacggaaa tggcaatact cagggtggaa
     7441 cccttgatat gataaatggt gcggacattg aacataatct ttttattagc agtacctccg
     7501 ggagtgctca gcgggccttt ggtgatgtaa ccaattgcaa tattagaaac aacattttct
     7561 acggaataag agcccaggcc agtaacacat ttacaggaaa caatattgac aataacatta
     7621 ctaacttttg ttctgataac acgattcagg ggggaaatgg aaatgttctc actaataacc
     7681 tggaaggcgt tgatcctcaa ttagtcaatg ttcccttcag tgtcaggtat aatttcagtg
     7741 acgatccaag cttaataccc gggtctccct gcgaaggtgc agccgatgac ggaactgata
     7801 tcggattatt tggcggaggc tctcctatgg atatta
//
LOCUS       DASWHX010000034         8444 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_1136244, whole genome shotgun sequence.
ACCESSION   DASWHX010000034 DASWHX010000000
VERSION     DASWHX010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 8444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8444
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1136244"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..280
                     /locus_tag="VGA21_01990"
     CDS             <1..280
                     /locus_tag="VGA21_01990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649307.1"
                     /translation="QTMILEMVNLSFEDLGYTFTIEEIQFEYDLESFFDFYKVINAKA
                     LSERIGMNQSLLAQYIKGIKKPSVAQTRRILKGVHQIGRELSEARFLV"
     gene            complement(430..501)
                     /locus_tag="VGA21_01995"
     tRNA            complement(430..501)
                     /locus_tag="VGA21_01995"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(466..468),aa:Glu,seq:ttc)
     gene            883..1506
                     /locus_tag="VGA21_02000"
     CDS             883..1506
                     /locus_tag="VGA21_02000"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEX9649308.1"
                     /translation="MKKNWKYPERASLEFKSIQLIPLDADLHSQILFEISKPEANRGV
                     DIFEFMLNTGPFINSEEFRSYLNKMNNDPLRLAYTVFSKRLDQFVGTASIMNINITHG
                     VAELGSIWYCTKAQRTEVNTETIFTFLNYLFDTLQYRRVEWKCNNVNQRSKDAAIRLG
                     FTFEGVFRHHMVDKGKNRDTAWYSIIDSEWADIRDHFEHKLLPGNHN"
     gene            1547..2131
                     /locus_tag="VGA21_02005"
     CDS             1547..2131
                     /locus_tag="VGA21_02005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649309.1"
                     /translation="MKKLIYVITFLILIPLMALARQTPQDLAYQREIEIAAGLMELGA
                     FDSADFHLRHVIQNMKVLPTKISFYFGKNSLSLKRYKQSINWLNKYMELAGTTGQFFD
                     ESVKLLEKAEEEYRLERNQEIEFMVSELHGEKGIKCEGTETIICPVCKGLGVVIKEKN
                     FGKEYWPCPFSDARGLLSCSDYNLLIEGKLKPRN"
     gene            2133..3167
                     /locus_tag="VGA21_02010"
     CDS             2133..3167
                     /locus_tag="VGA21_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002700073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HEX9649310.1"
                     /translation="MSVLHNKVNGKELKKALRESAQKRITLSFYKYLHILNARQFRDN
                     LYREWSKMSVLGRVYVAAEGINAQISIPAEAKKVFQDWIDSSSLNNTYLNFAYEDDGK
                     SFFKLKIQVKHKILADGIDAKVFDVTSPGTHLDAATFNRLTDDPETILIDMRNHYETE
                     VGHFKNAVLPDTDTFKETLKVVEEMIAGKEDKNIVMYCTGGIRCEKASAFYKSKGFVK
                     VFQLRGGIIKYANDAKSHGIPNKFIGKNFVFDERLGERITDDIISQCHQCGELCDTHV
                     NCKNDACHLLFIQCEKCARKYGNCCSEECREFIQLPEDEQKRLRRGRKLNQQIFRKGR
                     KLKMENHFLS"
     gene            3164..3697
                     /gene="mog"
                     /locus_tag="VGA21_02015"
     CDS             3164..3697
                     /gene="mog"
                     /locus_tag="VGA21_02015"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012930152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HEX9649311.1"
                     /translation="MTDIFIGIINVSDRASKGIYEDIPGKAIKETLGEYLVSPWKDVY
                     RVIPDEQEEIEAAMCEMADDLNCSLIITSGGTGPSFRDVTPEATKRVCQKMMPGFGEL
                     MRQVSLQYVPTAILSRQTAGIRNKTLIVNLPGKPKAIRQCLNAVFPAIPYCIDLIGGP
                     YLESNPDIIRVFRPKSD"
     gene            3841..4689
                     /locus_tag="VGA21_02020"
     CDS             3841..4689
                     /locus_tag="VGA21_02020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649312.1"
                     /translation="MRKKGMNFRNIWKLPLLAVLVFAGSCESDEAVLEPEEVKNIESS
                     IEMDAAYGDTDELAGLSMVLAEGGTLGGRVTEGDDRFGCAEVTHDADLKKIIINFGEG
                     CNDLRGHTRKGVIIITYTGHRFIPGSVITVRFEDYYFDGIKVEGVRTLTNITEGQDSP
                     PKFQIVLVEGKLTFPDETFVTRNMTEIRTWIRGANILQDEFHVEGSATGVGRNGVEYA
                     MEITETLVWKVSCTLNKVFLPVDGKKIVTRGQRVFEVDFGNGECDNIVVVTSNGASAT
                     VDLSRD"
     gene            complement(4742..6103)
                     /locus_tag="VGA21_02025"
     CDS             complement(4742..6103)
                     /locus_tag="VGA21_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012930488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEX9649313.1"
                     /translation="MKNLLIPFVISPLLSVAQSDSLIIRNIFNEALLNGKSYEMLDYL
                     TNKIGSRLSGSEGAAKSVEWAKMQMDELGFDKVYLQEVMVPKWVRGKEEVARITQTDG
                     TVTTVNVCALGNSVGTGASGISGRIVEVQDFETLANLGRENVVGKIVYFSRPMDSKHI
                     ETFRAYGGAVNQRGSGPAEAAKLGAIGTIVRSMTLAFDDHPHTGSTNYGEGENKIPAI
                     AISTMDGDHLSKLLKEDPELSFYFETHCEMQEEVLSFNVIGEITGKELPAEVIVVGGH
                     LDSWDLGTGAHDDGAGCVQSIEVIRVIKSLGINNKRTIRAVMFMNEENGLRGGRKYAE
                     DVIAKNEKHIAAIESDRGGFTPRGFGMTGTPEQLIKFQSWAELFAPYMISEFVAGGGG
                     ADIGPLRPTGTPLIGYIPDSQRYFDFHHSELDVFGGVNKRELEMGAAAMAALVYLIDK
                     HGL"
     gene            6215..7009
                     /locus_tag="VGA21_02030"
     CDS             6215..7009
                     /locus_tag="VGA21_02030"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011584676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEX9649314.1"
                     /translation="MQQYHELMKHILTSAVKKEDRTGTGTLSVFGYQMRFDLQKGFPM
                     ITTKKLHLKSIIYELLWFLNGDTNIKYLNENGVRIWDEWADENGNLGPVYGHQWRSWP
                     TPDGTTVDQISLLMDQIKKNPNSRRLIVSAWNVPEVNKMALPPCHTLFQFYVADRKLS
                     CQLYQRSADVFLGVPFNIASYSLLTLMVAQVCDLEPGEFVHTFGDAHLYSNHLEQAKL
                     QLTRTPRAFPMMKINPEVKDIFSFRFEDFELLNYDPYPHIKAEVAV"
     gene            7140..7817
                     /gene="sdaAB"
                     /locus_tag="VGA21_02035"
     CDS             7140..7817
                     /gene="sdaAB"
                     /locus_tag="VGA21_02035"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009578067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HEX9649315.1"
                     /translation="MAEKSGVFDMIGPVMIGPSSSHTAGVVRIGRVGLRVLGEPVKEA
                     QIVFYNSFARTYEGHSSDKAIIGGLLDFKTDDPRIKNAVEEAKERNLKFQFKSVGNAS
                     TLHPNTVKLNLRGDVRRVEIVGESLGGGVINISEIDGFSANFSASLHSLIIKANDVKG
                     SIGYIASVLANDECNIATMSVSRKGKHGLACQVIEMDSGLRPVTLEYLRSLNWIKEVI
                     YIPDIDL"
     gene            7829..>8444
                     /locus_tag="VGA21_02040"
     CDS             7829..>8444
                     /locus_tag="VGA21_02040"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX9649316.1"
                     /translation="MNTSKMISKLFKIAVICMIVSSPVLAQENPNAWSLEKCVEYAMQ
                     NNLSMELGRLGIESNRVAWQQNKANRFPTANLNGSYGSNWGRSINPTTNDFVARQNFS
                     SSWNLSSSVTVFNWGNINNRIKQADQNLEASQYDFEKTENDVILNVVSFYVNVIFNRE
                     LVENARLQLDNTEQQLDRINKQVQVGALPLTNLLDIQAQKSTNEL"
BASE COUNT         2664 a         1593 c         1795 g         2392 t
ORIGIN      
        1 tcaaacgatg atcctggaaa tggtgaatct ttcatttgaa gatttgggct atacctttac
       61 cattgaggaa atacaatttg aatatgacct tgaaagcttc tttgactttt acaaggtcat
      121 taatgcaaag gcgctttccg agcgcatcgg aatgaatcaa agtctattgg ctcagtacat
      181 taagggaatt aagaaaccat cggttgcaca aaccaggcgc attttaaagg gcgttcatca
      241 gataggcagg gaactttccg aagctcggtt tctggtatga gtgtgttatg cttttgagtg
      301 taacctgaat cacattttta ggttgaatga aaacattcta atcatccttg caaatctacc
      361 cataggtcta cccaagcaat aaaaaaaccg ctaataatct gataattagc ggtttaattc
      421 aattcctagt agcccgtagg ggaatcgaac ccctgttacc aggatgaaaa cctggcgtcc
      481 taacccctag acgaacgggc ctttaaaaag tgttgcaaag atagacctaa ccctattatt
      541 tgcaaaacac caccggcaat ttataaattt caattccagg taaatccaaa ccatcaattt
      601 tgctaaaatt gaagtcagtt tatattaacc ttgatttgtc atgtttcaag atttggttat
      661 ttctgccgtg tccctctcct gtggagaggg atttagggtg aggttaaatt ccgagaatag
      721 aaaactcaac ttgacaaagt aggattcatc ttgattttca ttttataaaa ttcttgaaaa
      781 tggataagtc caacaggcaa agttatctca gttggcttga aattttttca taatgaaggc
      841 taattgaaag gtttattcca tttttacttt tccctgcagg caatgaaaaa aaactggaaa
      901 tatcctgaac gagcgtctct cgaatttaag tccattcaac taattccact tgatgcggac
      961 cttcattctc aaattctttt tgaaatctca aaacctgaag ctaatcgggg agttgatatt
     1021 tttgaattta tgctgaatac cggcccattt attaattctg aagagttccg gagttactta
     1081 aacaaaatga ataacgatcc tctcagactt gcatacacag ttttcagtaa aaggctagat
     1141 caattcgttg gtactgcatc tataatgaat atcaatataa ctcatggcgt ggctgagctt
     1201 ggatcaatct ggtactgcac gaaagcccag cgaacggaag tgaatacgga aaccatattt
     1261 acatttctta attacctttt cgatacgctt caataccgca gggtagaatg gaaatgcaat
     1321 aatgttaacc aaagaagcaa ggatgccgcc attcgacttg gatttacttt tgaaggcgtg
     1381 ttcagacatc acatggtcga caaaggaaaa aacagggata ccgcctggta ctcgattatc
     1441 gattcggaat gggcagatat cagggatcat tttgagcata aattattgcc tggtaaccat
     1501 aattagtcgc cctttctgcc aaattaccta tctttgtgtt ttgaaaatga agaaattaat
     1561 ttacgttata actttcctga ttttgattcc tttgatggcc cttgccaggc aaactccaca
     1621 ggatctggcc taccagagag aaattgaaat tgctgccggg ctgatggaac tgggtgcctt
     1681 tgatagtgca gactttcacc ttcggcatgt tattcaaaat atgaaagtgc tgcccacaaa
     1741 gattagtttt tatttcggaa aaaattctct gtctttaaag cgatacaaac aaagcatcaa
     1801 ctggttgaat aaatacatgg aacttgcagg taccaccgga cagttttttg atgaatcagt
     1861 taaattattg gagaaggccg aagaagaata caggctggag cgaaaccagg agatagaatt
     1921 catggtgtcg gaattgcatg gagagaaagg aataaaatgc gaagggacag aaacaataat
     1981 ctgtcccgtc tgtaagggat taggcgttgt aattaaggaa aaaaattttg gaaaagaata
     2041 ctggccctgc ccatttagtg atgcccgggg tcttttaagc tgctccgatt ataatttatt
     2101 gatagaaggt aaattgaaac ctagaaatta agatgtcagt ccttcacaat aaggtaaatg
     2161 gcaaagagct taagaaggct ttgagagaaa gcgcccagaa aagaattacc ttgtcttttt
     2221 ataagtatct gcatattcta aatgcgcggc aattccggga taatctctat agggaatggt
     2281 caaaaatgag tgtactggga agagtgtatg tagctgcaga gggtataaat gcgcaaatca
     2341 gtattccggc agaagctaag aaagtatttc aagactggat tgattcctca tctctaaata
     2401 atacttatct aaactttgct tacgaagatg atggcaaatc tttttttaaa ctgaaaatac
     2461 aagtgaaaca taagattctg gctgatggaa ttgacgccaa agtttttgat gtaacctcac
     2521 cgggaacgca tcttgacgcc gccacattta accggctcac cgatgatccg gaaacgattc
     2581 taattgacat gagaaatcat tatgagacag aagttggtca tttcaaaaat gcagttttgc
     2641 cagatacgga tacttttaag gaaaccctga aagtggtgga agaaatgatt gccggtaagg
     2701 aagacaaaaa tatagttatg tattgtaccg ggggtatcag gtgtgaaaag gcgagtgcct
     2761 tttataaatc aaaagggttt gtgaaagtgt ttcagttaag aggtggaatt atcaaatatg
     2821 caaatgatgc aaaatctcat ggaatcccaa acaagtttat tggaaagaat tttgtttttg
     2881 atgaaagatt gggggaacga ataacagatg atatcataag ccagtgtcat caatgcggag
     2941 aactctgtga tacccacgtg aattgtaaga atgatgcctg ccatttatta tttatccagt
     3001 gtgaaaagtg tgccaggaag tatggaaact gttgttctga agaatgcagg gaatttatcc
     3061 agcttccgga agatgagcag aagcgtttgc gtagaggacg aaaattgaat caacaaattt
     3121 tccgtaaggg gagaaaactt aagatggaaa accatttcct ttcatgacag atattttcat
     3181 tggcataatt aacgtttccg atcgggcaag caaggggata tatgaagaca tccccggcaa
     3241 ggccattaaa gaaaccctgg gtgaatatct tgtctcgccc tggaaagatg tttacagagt
     3301 tattccggat gaacaagaag aaattgaggc agcgatgtgt gagatggctg atgacctgaa
     3361 ttgctctttg attataacca gtggcggaac aggaccttcg ttcagagatg taactccgga
     3421 agccacaaaa agagtttgtc aaaaaatgat gcctggtttt ggtgaactga tgcggcaagt
     3481 gagtctgcaa tatgttccga cagcaattct gtccagacaa actgccggaa ttcgaaataa
     3541 aacgttaatt gtgaatctac caggtaaacc aaaagcaata aggcaatgtc tcaatgcggt
     3601 ttttcccgcg attccctatt gcatagacct gattggaggc ccatatctgg aatcaaaccc
     3661 cgatataatc agggtcttcc gtcccaaatc agattaaata aaagaaccta aattccttta
     3721 aaagaagggg tttttattaa cgcaggttac catcctgaaa attgagggat aaacattcca
     3781 aagaccccat taaacttttc aacccattct tagtctaata acaaatcaat attgaaaata
     3841 atgagaaaaa aaggaatgaa tttcagaaac atttggaagt taccattgct tgcagtgtta
     3901 gtttttgcag gctcttgcga atcagatgaa gcggtactgg aaccggagga agtaaaaaat
     3961 attgaatcca gcattgagat ggatgcggct tatggagata cggatgaatt ggctggcctt
     4021 tctatggtgt tggctgaagg aggaactctc ggcggaagag ttacggaagg tgatgaccgg
     4081 tttggttgtg ccgaggtaac ccatgatgct gatctcaaga agatcattat caattttgga
     4141 gaaggatgta atgatttaag aggtcacacc agaaagggtg taataatcat cacctatacg
     4201 ggacacaggt ttatcccggg atctgtaata acggtgcggt ttgaagatta ctattttgat
     4261 ggaattaaag tagagggcgt cagaactttg accaacatta ccgaaggaca agattctccg
     4321 cctaaatttc agattgtcct ggttgaagga aagcttactt ttcctgacga aacttttgta
     4381 accagaaata tgacggaaat aagaacctgg attcgcggcg cgaatatcct gcaggatgaa
     4441 ttccatgtgg aaggaagtgc taccggcgta ggtagaaatg gagtggaata cgctatggaa
     4501 attacagaaa ctttggtatg gaaggtatcc tgcactctga ataaggtatt tctaccggtt
     4561 gacggtaaga aaatagttac aagaggccag cgagtgtttg aagttgactt tggtaatggt
     4621 gaatgcgata acatagtcgt ggttaccagc aacggagctt cagcaaccgt tgatctgtct
     4681 cgcgattaat ttagttttgg tggttagatt atgattaagg ccggccaatt gccggctttt
     4741 tttataaccc gtgcttatca ataaggtaaa ccaatgccgc cattgctgca gcccccattt
     4801 ccaattcacg tttatttact ccaccaaata catccagttc agaatggtga aaatcaaaat
     4861 atcgttgaga atccggaata tacccgataa gaggagtacc ggttggtcgg agaggtccga
     4921 tatcagctcc accgccgccg gcaacaaatt ctgaaatcat atagggagca aacaactcag
     4981 cccaactttg aaatttaatc agttgttcag gtgttccggt catcccaaat cctctgggag
     5041 taaaaccacc acggtcagac tcaattgcgg ctatatgttt ttcatttttt gcgattacat
     5101 cctctgcata ttttcttccg ccacgtaatc cattctcttc attcataaac attaccgccc
     5161 tgatggtacg tttattatta atccccaaac tttttattac cctaattact tcaatggatt
     5221 gaacacagcc tgctccgtca tcatgagcac cggtgccaag gtcccaggaa tcaagatgtc
     5281 caccaaccac tataacttcc gcaggcaact ctttgcccgt tatttcacca atgacattaa
     5341 atgacaacac ttcttcctgc atttcacaat gagtttcaaa atagaaactt agctccggat
     5401 cctctttcag tagtttgctt aaatgatctc catccatagt gctgatagca atggctggaa
     5461 tcttattctc cccttcaccg taattagtag aacccgtatg aggatgatca tcaaaagcta
     5521 aagtcatgga tcgaacaatt gttccgattg cgcccaactt tgcggcttca gcaggacctg
     5581 aacctctttg attcacagcg ccgccatatg cacgaaaagt ttcgatgtgc ttcgaatcca
     5641 taggtctgct gaaataaaca atctttccta ccacgttttc gcgacccaga tttgccaatg
     5701 tctcaaagtc ctgaacctcg acaatcctcc cggatattcc ggaagcacct gtaccaacag
     5761 aattaccaag ggcgcagaca tttaccgttg tcacagttcc atctgtttgg gtaattcggg
     5821 ccacttcttc ctttccacga acccattttg gcaccatcac ttcttgcaga taaaccttat
     5881 caaaacccaa ttcatccatt tgcattttag cccactcaac tgacttcgct gcaccttcgg
     5941 aaccactcaa cctcgaacca atcttatttg tcaggtaatc aagcatttca taagattttc
     6001 catttaacaa ggcttcatta aatatgtttc tgataatcag agaatcggat tgagctactg
     6061 aaagtaacgg actaatcaca aaaggaatta aaagattttt cattatttgc tttttttcaa
     6121 tcaccaaagg taagtcagag aaattaatgt ggaaactaaa tttaattttt agggttaacc
     6181 tattaatttt gttcatcaac cagagtacca gataatgcag cagtatcatg aattaatgaa
     6241 acatatcctg accagcgcgg taaagaaaga agacagaaca ggaacaggaa cactcagcgt
     6301 tttcggatac caaatgcggt ttgatttgca gaaaggattt cctatgataa ctacgaagaa
     6361 attgcacctg aaatccataa tctatgaatt gctttggttc ctcaatgggg ataccaatat
     6421 taaatatttg aatgaaaacg gggttagaat ttgggatgaa tgggcggatg aaaatggcaa
     6481 cctgggtcct gtctatggtc atcagtggcg tagctggccc accccggatg gtaccacggt
     6541 tgatcagatc tctttattga tggatcaaat taagaaaaat cccaattcca gaagattaat
     6601 tgttagtgcc tggaacgtgc ctgaggtaaa caaaatggct ttgccgcctt gtcatacatt
     6661 gttccagttt tacgttgccg acagaaaact ttcctgtcaa ctttatcagc ggtcagcaga
     6721 tgtatttctt ggggttccat ttaatattgc aagttattca ttactaacgc tcatggtggc
     6781 tcaggtttgt gatttagaac ctggagaatt tgtccatact tttggtgatg cgcatttata
     6841 ctctaaccat cttgaacagg cgaaactcca gttaacaaga actcccaggg cttttccaat
     6901 gatgaaaata aatccggaag taaaagatat tttttcattc aggttcgaag actttgaatt
     6961 actgaactac gatccttatc cgcatattaa ggcagaggta gctgtttgat ttccgtacta
     7021 aatcctcatc attggtagcc gaataaggag gcttataagg aaaaattacc atttatacat
     7081 tatgtaagcc tcgccagccc tttaatcgtt aatattgcaa acaacccaat gggaggtaaa
     7141 tggccgaaaa aagcggtgtt tttgatatga taggccctgt tatgataggt ccatccagct
     7201 ctcacacagc aggtgtggtt agaattggca gggtaggttt acgggtatta ggtgaacctg
     7261 ttaaggaagc acagattgtc ttttataatt cctttgccag aacctatgaa gggcatagca
     7321 gtgacaaggc aataattgga ggtctgcttg attttaagac agacgatccg agaatcaaaa
     7381 atgccgtgga agaggccaaa gaaagaaact taaagtttca gtttaaatct gtcggcaatg
     7441 cttcaacact tcatcccaat accgttaaac taaatttacg gggagacgtt agaagggtgg
     7501 agattgtagg tgaaagttta ggaggtggag taataaacat tagtgaaatt gatgggttta
     7561 gtgccaattt cagcgcaagc cttcattccc tgattataaa ggcaaacgac gttaaaggct
     7621 ccattggtta catcgcgagt gttttagcga atgatgaatg taatattgcc acgatgtcag
     7681 tttcaaggaa aggaaaacat gggttggcgt gccaggtaat tgagatggat tccgggcttc
     7741 gacctgtaac attggagtac ctgagaagtc taaattggat aaaggaagtt atctatatac
     7801 cagacattga tttataaaac ttaactaatt gaacacatca aaaatgataa gcaaactatt
     7861 taaaattgca gttatatgca tgattgtttc ttctccggtt ttggctcagg aaaacccaaa
     7921 tgcatggtcg ctggagaaat gtgtggaata cgccatgcaa aacaacctgt caatggaatt
     7981 ggggagattg gggatagaat ccaacagggt tgcatggcag caaaataaag ctaatcgatt
     8041 tccgacagca aatcttaatg gatcttacgg gagcaattgg gggcgttcaa ttaacccgac
     8101 tacgaatgac tttgtagctc gtcagaattt ttcctcttca tggaatttaa gcagctctgt
     8161 gacagtattc aattggggta atataaacaa tagaattaaa caggccgatc aaaacctgga
     8221 ggcaagccaa tatgatttcg agaaaaccga gaatgatgtt atcctcaatg tagttagttt
     8281 ttatgtgaat gtaatcttca atcgggagtt ggttgaaaat gcaagacttc aattagataa
     8341 cacagagcaa caactggata gaatcaataa gcaggtgcaa gtgggtgcac tccctcttac
     8401 aaatttgctg gacattcagg ctcaaaaatc taccaatgag ttaa
//
LOCUS       DASWHX010000035         6105 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_1136291, whole genome shotgun sequence.
ACCESSION   DASWHX010000035 DASWHX010000000
VERSION     DASWHX010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 6105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6105
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1136291"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..280
                     /locus_tag="VGA21_02045"
     CDS             <1..280
                     /locus_tag="VGA21_02045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649317.1"
                     /translation="QTMILEMVNLSFEDLGYTFTIEEIQFEYDLESFFDFYKVINAKA
                     LSERIGMNQSLLAQYIKGIKKPSVAQTRRILKGVHQIGRELSEARFLV"
     gene            complement(381..728)
                     /locus_tag="VGA21_02050"
     CDS             complement(381..728)
                     /locus_tag="VGA21_02050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649318.1"
                     /translation="MTNEEKIKIRNIISSAFVELGRIKQILATKGHNYEYPESVFLKE
                     PEMDNQNILIYEIDGLNQLWQASEIMRDKMEDVLALEHSDFLQVSQFIESNWKFIFEG
                     LKKIEKSLNAKQI"
     gene            complement(806..880)
                     /locus_tag="VGA21_02055"
     tRNA            complement(806..880)
                     /locus_tag="VGA21_02055"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(845..847),aa:Arg,seq:cct)
     gene            1293..1499
                     /locus_tag="VGA21_02060"
     CDS             1293..1499
                     /locus_tag="VGA21_02060"
                     /inference="COORDINATES: protein motif:HMM:NF024835.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX9649319.1"
                     /translation="MKTGILVRFGSKIRALRQERNWTQEKLAELTGFHPTYIGRVERG
                     ERNLSLKNIEIFAGVFKLQIKDLF"
     gene            complement(1549..2895)
                     /locus_tag="VGA21_02065"
     CDS             complement(1549..2895)
                     /locus_tag="VGA21_02065"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009579292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HEX9649320.1"
                     /translation="MINHLVITLFIFSLAIESLFGQVKNIVLDATGQGRAPAEPSIAI
                     NPTDINNIVAGAILDKVYVTKDGGVTWSTQQLSSPYGVWGDPVIITDEKGNFYYFHLS
                     DPTGENWSSEEILDRIVVQSSGDGGDTWDEGSYTGMNHPKDQDKPWADYSPALKRLLV
                     SWTQFDKYNSADPNHKSNIHFSYSSNRGKKWSKPVRINQVSGDCLDDDLTTEGAVPVG
                     GVNGEIYIAWAHGEKIYLDRSMDKGKTWLGNDIVVSDLPGGWSIDIPGVYRCNGMPVL
                     MVDRSKLGLKGMLYVVWSDQRNGSDDTDIWITRSSNGGDRWTSAVRVNDDPAGKHQFL
                     PWPVIDQVTGYIYVVYYDRRNYDDNNTDVFLAYSTDGGVTFKNKRISEKPFTPQKDKF
                     LGDYINVAAHDGRIVPIWTYMEDGKTSIFTAILSHSDLGLPEIKRVEKKKRKNEEK"
     gene            3060..3662
                     /locus_tag="VGA21_02070"
     CDS             3060..3662
                     /locus_tag="VGA21_02070"
                     /inference="COORDINATES: protein motif:HMM:NF019408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1629 domain-containing protein"
                     /protein_id="HEX9649321.1"
                     /translation="MRCFKLETLGYGRINGKSLAIVDDISPLDSAIKLRAMIGWGESL
                     KGLHPKNAEIRIEKLKNNGALSNLIGNTTNILLVDKIMKEIIRKLEPLESKIEIFPFK
                     LFSSKGKLLSDELYHINPVGLVDCLDLEKSEIKYHEEDGKKELVRIKEYVFRKDKLEH
                     ASCIFKIPEDPSSCFIRIDLARELQKANCENLCVLEIKVS"
     gene            complement(3751..5163)
                     /locus_tag="VGA21_02075"
     CDS             complement(3751..5163)
                     /locus_tag="VGA21_02075"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEX9649322.1"
                     /translation="MFRILLLLILLVTPLLSLQAQNRDAMDSLQNLLSTQSEPLRKAS
                     LLEELSLAQYNNGLITPAIRSLKQSNRIRGVYNSKEKLIDNLLTISSYYQQLNNISMS
                     VVYLRLYSLVRQEYKEGILLNRISDLERLQGLEITALEEQRDRAVAESLELANQLVNI
                     QKKLNIFQIAAISIFIVFLTGAILTFVYLIRKNPGAAASGTPDPELFQQIQILEKEVN
                     DLKTYLHSGLYLTGQLDDLEKSSENALKKIFPDLLLYNKPRSGSGSNFLWHTIDSNKA
                     ILIIGQTNISGLNGSLISILVRKILNHVILEEKIVTPSMVLTLVDQKLRHCIGNESEL
                     AYIGCGMTYCLINLAAREMEFAGAGLHLYQIQNKKGNVLKGSLISLANSFAQEKFFNT
                     HRLTITANEGIFLSTSGILDQVGQSEDKRFGRKSFIKLLVTLADKKSSDQYYILDKVH
                     KEWKGMQPQTDDILVVGLRF"
     gene            complement(5224..>6105)
                     /locus_tag="VGA21_02080"
     CDS             complement(5224..>6105)
                     /locus_tag="VGA21_02080"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HEX9649323.1"
                     /translation="DIVPHSEGGRIIGFIFLFLSLGTYAFLIGQISSIMNNIKENRKL
                     GQFGTRFSNHVVMIGWTEFGKSVTDELLGAGRDVAIVTSDKNNVDLIREVYSTSKVFV
                     LFTDYHNVDQLTKVNIEESSNVFINLQDDTEKLVYILNMKKTYPGLKYVVTLENANLK
                     NTFLTAGVTYAISKNELASKLLASYIFEPDVAILNEEILAYADTDEKYDMKEYKVLEN
                     NPYRNKKYGTVFYDLKKECNVILVGLVKNQEGERKLFKNADDEMLVEAGDYLIMLMNK
                     IAVKKLTSVFKTSEGLD"
BASE COUNT         1820 a         1258 c         1141 g         1886 t
ORIGIN      
        1 tcaaacgatg atcctggaaa tggtgaatct ttcatttgaa gatttgggct atacctttac
       61 cattgaggaa atacaatttg aatatgacct tgaaagcttc tttgactttt acaaggtcat
      121 taatgcaaag gcgctttccg aacgcatcgg aatgaatcaa agtctattgg ctcaatacat
      181 taaaggaatt aagaaaccat cggttgccca aaccagacgc attttaaagg gcgttcatca
      241 gataggcagg gaactttccg aagctcggtt tctggtatga gtgtgttatt tgcttttgag
      301 tgtatctgaa tcacagtttt aggttgaatg aaaacattct aatcatcttt gcaagtctcg
      361 ataaaaaaag gttatctgcc tcagatttgt ttggcgttca aacttttctc tatcttcttt
      421 aatccctcaa atataaattt ccaattagac tcaataaatt gtgagacttg gaggaaatca
      481 gaatgctcaa gagctaagac atcttccatc ttatctctca taatttcgga agcttgccaa
      541 agctgattta acccatcaat ctcataaata aggatgttct gattatccat ttctggctcc
      601 tttaaaaata ctgattcagg atattcataa ttatgccctt tagtagccaa aatttgttta
      661 atcctaccaa gttcaacaaa ggcactggag attatatttc taatcttgat tttctcctcg
      721 ttggtcatac aagtctaccc ataggtctac ccattcaaat aaaaaaccgc taaataactg
      781 aatatcagct gattaagtaa tgtcctggta gccccgacag gaatcgaacc tgtatctaga
      841 gtttaggaaa ctcctattct atccattgaa ctacggggcc aatgttggaa agatgcgcaa
      901 aaataagaaa atccctgtgg aaggtggaag tatacatcgg gataaatgca tatgaaggtc
      961 gtcctacgta atcctggcga tcactccccg ttaactttca gcaaattttt cattccggga
     1021 tcgagttctt gtccaatctt gattaagtac cggaaagaat agttaatctc cccgttttca
     1081 atgcttgtca atttagactc tcacatattt tgatcttttt tagcaaattc ttgccttgaa
     1141 tatccgtttg aagaccttag ccttttaata ttgagtccaa tttttttcct gatgagttcg
     1201 atataatact ccataaaaat cccaccaata tacaaaatga acatgactca ttggctgtgt
     1261 attatgattc attgcaggta ttcctttgga aagtgaaaac aggaatatta gttagattcg
     1321 gctccaaaat cagagctttg cggcaggagc gaaactggac tcaggaaaaa cttgctgagt
     1381 taaccggatt tcatcctact tatatcggac gggttgaaag gggtgaaaga aatctttccc
     1441 taaaaaacat tgaaattttt gccggagttt tcaaacttca aattaaagat ttgttttaga
     1501 atttcaattc caaactgttg cttctctgac atacctctgg caacaatttt acttttcctc
     1561 gttctttctt ttctttttct ccaccctttt tatttcgggc aaccctaaat cggaatgaga
     1621 gagaatggcc gtaaaaatgc tggtctttcc atcctccatg tacgtccaga ttgggacaat
     1681 tctaccatcg tgtgccgcaa cattaatata gtcacccagg aatttatctt tctgaggagt
     1741 gaatggcttt tcgctaatcc tcttattctt aaaagtaaca ccgccatcag tggaataggc
     1801 caggaaaaca tcggtattgt tatcgtcgta gttacgacgg tcgtagtaaa caacgtaaat
     1861 atacccggtt acctgatcga tgaccggcca tggcaaaaac tggtgttttc cggcgggatc
     1921 atcatttaca cgaaccgctg aagtccaccg gtcgccacca ttggaagaac gggtaatcca
     1981 gatatcggtg tcatcactgc cattgcgctg gtcagaccaa accacgtaaa gcattccttt
     2041 caagccgagt ttacttcggt caaccattaa tacaggcatc ccattacatc ggtaaactcc
     2101 gggaatgtct atgctccatc cgcccgggag gtcggaaact acgatatcgt ttcccagcca
     2161 ggttttccct ttatccatgg aacgatccaa ataaattttt tcaccatgag cccatgctat
     2221 gtatatttct ccatttaccc ctcctacagg tacagcaccc tcggtggtta aatcatcatc
     2281 caggcaatct cccgaaacct gatttatgcg aacaggttta gaccattttt tccctctgtt
     2341 tgatgagtag gagaaatgta tattgctttt atggttggga tcggcactgt tgtatttgtc
     2401 aaactgtgtc cagcttacca ggagtcgctt taatgccgga ctgtaatctg cccagggctt
     2461 gtcctgatct tttgggtgat tcattccggt gtatgaaccc tcgtcccagg tgtcaccacc
     2521 atctcccgaa gattgaacta ctatgcgatc caaaatttct tcgctgctcc aattttctcc
     2581 cgtgggatcc gagagatgga aataatagaa atttcctttt tcatcagtaa tgatgaccgg
     2641 atctccccac actccatacg gagaagaaag ttgttgagtt gaccaggtaa caccgccatc
     2701 tttggtaaca tatactttgt caagaatggc acctgccact atattattta tatccgtagg
     2761 attgatggca atgctcggct cagctggcgc tcttccctgg ccggttgcat ccagcacaat
     2821 attcttcacc tggccgaata gagattctat ggccagggaa aaaatgaaaa gagtaattac
     2881 cagatgattt atcacagatc aatttttgct aaaataggaa gaaggtgtca attaaacaga
     2941 gccaaagcag cactaaatga aatgatagtt acaatatttc cagtctttgg gaaataatta
     3001 catgagataa tcaataaaaa gatcaaattt taaaatccaa aaactaaaca agtaaaacaa
     3061 tgcgatgttt taaattagaa acattaggct acggccgaat aaatggtaaa tcactggcta
     3121 tagttgacga catttcgcca cttgacagtg ccatcaaact gcgtgctatg attggctggg
     3181 gcgaaagcct aaaaggtctt catcccaaaa atgctgaaat acggatagaa aaattaaaga
     3241 ataatggagc tctttcaaat ttgattggca acacaacgaa tatattattg gtagataaga
     3301 ttatgaaaga gataattaga aaattggagc ctttggagag caagatcgag atattccctt
     3361 tcaaattatt ttcttcaaaa ggcaaattgc taagtgacga gctttaccac attaatccgg
     3421 taggtttggt tgattgtctg gacctcgaga aaagcgagat caaataccac gaagaagatg
     3481 gcaagaaaga actcgtcagg attaaggagt atgtcttcag gaaggataaa cttgaacatg
     3541 cctcttgtat attcaaaatc cctgaggatc catccagttg ttttattcgc attgatttgg
     3601 caagagaact tcaaaaggca aactgtgaaa atttatgtgt tttggagatc aaagtttcat
     3661 aaccagcagt ggagttggca aaatatatag agctgacaat taacatttaa tacggctttg
     3721 cctgacgttg aaaacgtact aagataattc ctaaaatctc aatcctacaa ccaaaatatc
     3781 atcagtttgc ggctgcattc ccttccattc cttgtgcact ttgtcaagta tgtaatattg
     3841 atcagaagat tttttgtcag caagcgtcac cagaagctta ataaacgatt tcctgccaaa
     3901 ccgtttgtct tcgctctggc ccacctggtc taaaatcccg gaggttgaca gaaagattcc
     3961 ttcatttgca gttatggtta atctatgagt attaaagaat ttttcctggg caaaagagtt
     4021 tgccaatgaa ataaggcttc ctttcaagac attacctttt ttattttgaa tttgatacaa
     4081 atgaagtcct gctccggcaa attccatttc cctggcagca agattaatca gacagtaggt
     4141 catcccgcat cctatatatg ccaactcact ttcattgcca atgcaatgtc tgagtttttg
     4201 atcaaccaga gtcaatacca tggatggagt tacaattttt tcctccagga ttacatgatt
     4261 taaaattttc cttaccagga tggaaatcag gcttccattt aacccggaaa tattggtctg
     4321 accaataatt agtattgctt tgttcgaatc aatcgtgtgc cagagaaaat tactgccgga
     4381 gccggagcgt ggtttgttat aaagcaataa atccggaaag attttcttta gagcattttc
     4441 tgaagatttc tccagatcat ccagttgacc tgttaggtat aagccggaat gcaggtaggt
     4501 tttcaaatcg tttacttctt tttcaaggat ttgaatttgc tgaaacagtt ccggatcagg
     4561 agttccggag gcggcagcac cgggattttt tctgattaaa taaacaaatg tcagaattgc
     4621 tcccgtcaga aaaacaatga atatggaaat tgcagcaatc tgaaaaatat ttaatttctt
     4681 ttgaatgttg accaactgat tggccagctc aagactctcc gcaactgccc ggtcgcgctg
     4741 ctcttcaagg gccgtaattt ccaacccctg caatcgctcc agatctgaaa ttctgtttag
     4801 caaaattcct tccttatact cttgccttac caggctgtaa agccggaggt acaccactga
     4861 catggaaatg ttattcaatt gttgataata actggaaatg gtgagaagat tatcaatgag
     4921 tttttctttg ctgttataaa ctccgcgaat ccggttactt tgtttcagtg acctgattgc
     4981 aggtgtaatc aggccattat tatattgcgc gagtgacaat tcttcaagga gacttgcttt
     5041 cctcaaaggt tcagactggg ttgaaagtaa attttgcaaa ctatccattg catcccggtt
     5101 ttgcgcctgc aatgaaagga gaggagtaac caataatatt agaagcagga gaattctgaa
     5161 catgccatta aatatagcta attcataatt ggagcctgga gattgttagc aagattgtct
     5221 gtatcaatcc aaaccctctg atgtcttaaa tacgctggta agttttttaa ctgcaatttt
     5281 gttcatcaac ataataagat aatcgccggc ttcaaccagc atttcatcat cagcattttt
     5341 aaataatttg cgctcacctt cctgattttt taccagtcca accagaatca cattgcattc
     5401 cttctttaaa tcataaaaga cagttccata cttcttgtta cgatagggat tattttccag
     5461 gaccttatat tctttcatat catatttttc atccgtatcc gcgtaagcga gaatttcttc
     5521 attaagtatg gcaacatcgg gttcaaaaat gtagctcgcc aatagctttg aagccagttc
     5581 attcttggaa attgcatacg ttactcccgc ggttaggaaa gtgtttttca ggttggcatt
     5641 ttccagggta acaacatact ttaggcccgg atatgtcttt ttcatattca gaatatagac
     5701 cagcttttca gtatcatcct gaaggttgat aaagacattg gaactttctt cgatgtttac
     5761 tttagtaagc tgatcaacat tgtgataatc tgtaaacagg acgaacactt tacttgtgct
     5821 gtatacttct cttatcaggt caacgttatt tttatcgctt gtaacaatag ctacatccct
     5881 gcctgcaccc agaagttcat ccgttacgga tttaccaaat tccgtccaac caatcattac
     5941 aacatgattt gagaaccggg ttccaaactg gcccagcttt ctgttttcct ttatgttatt
     6001 cataatgcta ctgatttgtc caattaagaa tgcatacgtt ccaagactaa gaaaaagaaa
     6061 gataaatcca attattctcc caccctccga gtgagggaca atgtc
//
LOCUS       DASWHX010000036         4134 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_1497680, whole genome shotgun sequence.
ACCESSION   DASWHX010000036 DASWHX010000000
VERSION     DASWHX010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 4134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4134
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1497680"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            157..1053
                     /locus_tag="VGA21_02085"
     CDS             157..1053
                     /locus_tag="VGA21_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009285708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9649324.1"
                     /translation="MKILITGSNGLLGQKLLALCLRKGTEVIATSLGRNRFDDSLTYL
                     TMDITNSAEVEEVIGKTKPDVIINTAAMTNVDQCEDEKERCLLLNVAAVKNLVQSCEK
                     HNSFLVHLSTDFVFDGSHGPLNENDKPNPVNFYGESKLKSELLIQESKCGWAIARTIL
                     VYGVLQNMSRSNIILWVKESLEKGIEIQVVDDQFRTPTLAEDLADGCYLIATKQAAGI
                     WNISGKDLLTPYQMAIKTAEFFNLDQNLIKRTNSSAFRQRAVRPLKTGLVIEKAQSQL
                     GYNPHSFEEGIQIVAEQMSQPN"
     gene            1145..2533
                     /locus_tag="VGA21_02090"
     CDS             1145..2533
                     /locus_tag="VGA21_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008198876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HEX9649325.1"
                     /translation="MSTSPSSGDRGQWGSSFGFIMAAAGSAVGLGNIWRFPYITGQNG
                     GGAFVFVYILCVLLIGLPLIYNEIALGRITGKSTIGAFRDTGSNKFFMIFGAILALMV
                     SFFVLSYYGVIAGWTIGYIVSEFTHFIKDFDTFRSSSALVLPFFGLFMVLTIGIVLGG
                     VSGGIEKASKILMPVLFILLIGVMIRSLTLPGAGEGVRYYLYPDFSKITGRVVLAALG
                     QAFFSLSVGWGILITYGSYLPKSQNIVKSGMWIGLMDTGVALLAGLMIFPAVFAFSKD
                     PASGTTLVFQVLPEIFDSMPAGGNVVGALFFLLLCIAALTSAISMLEVPASYLIDESK
                     WSRKKSAWTVGILAFLVGIPSALSGGASHLFTELTIPYFGGTVKTGFLDIMDAIFGEL
                     FIVVVALMTSVYAGWFTNTRKLADEIAHGSKLFNKRIAGSLTWSGLWVFFIKYVCPIV
                     IGLVLLSTTGII"
     gene            complement(2534..>4134)
                     /locus_tag="VGA21_02095"
     CDS             complement(2534..>4134)
                     /locus_tag="VGA21_02095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HEX9649326.1"
                     /translation="GSQISLFNKSKDLVFQTQADSVKDLGNGFIRISVQGKQGLIHKN
                     GYVFFEPSLRDIELLSNSIFKFRRAEGWGLMDPFQKIIISDNLTSITGYGKIAALEKN
                     GKLGLYNIEELYNQTNTQSFQFPFEDVEQLTGGEFLSFSANQEVLLDENLKEIIPAAT
                     QEISHFKNYWISKGEVTTKLFSHSGKILVDSIQNYSIYGSKLGYKRNARWRIFETAES
                     KNTEYDSLKILSDNIYVSFSEKKNLAHFENNQLLDFSISGNYKLLKQQAPLGKSFLLT
                     FDKDGVKRLYNSEGKKILAGWFDEVKPINDFLFLVEEKKLSGLSDSTGQNVLPIEYEG
                     IGTYNNGFVATLKKGKFGVFHYPSRKVIQPQFDTRPQPYGDSLVIASKNGKSGIINVN
                     NKIILPFDYTKIEFWSNTQALVSKGNNFQLKNLQNDDSISLLNLKRVKLPNAKEVIIY
                     STLRGSGVASTGIGDIIPAVFSEINYLRTENEGIFVCGKFLAETDLHIVAYFDLKGEL
                     IWRSPVSREDYYKYLCLENSAGAN"
BASE COUNT         1222 a          760 c          857 g         1295 t
ORIGIN      
        1 gtggaaggct tggatgtgat tgataaaatt gcggcagtgc caacggatca gtcggaacgt
       61 ccgacaaccg atattatcat gatcataaca ctggaagaaa ttacaaaaaa ggaaatagcc
      121 aggaaatatg ggtactccta ctcagaaaac taattcgtga aaatcctgat tacaggctct
      181 aatggattat tagggcaaaa acttcttgcc ctttgtctca gaaaaggaac cgaagtaatt
      241 gcaacttcac ttggaaggaa caggtttgat gattctctta catatttaac aatggatata
      301 accaattccg ctgaagtgga agaagtaatc ggaaaaacaa aaccggatgt cataatcaac
      361 acagctgcga tgacaaatgt tgaccagtgc gaagatgaaa aagagagatg ccttctgctt
      421 aacgtagctg cagtaaagaa tctggtacaa tcatgtgaaa aacataattc atttcttgtt
      481 catctttcta ctgactttgt ttttgatgga agtcatggac ctttaaatga aaatgacaaa
      541 ccaaatccgg ttaattttta tggtgaaagt aaattgaaat cggaactgct gattcaggaa
      601 agtaaatgtg gatgggcgat cgcgagaacc attttggttt atggcgtatt gcaaaatatg
      661 agccgctcaa atataatctt atgggtaaag gagagcctgg agaagggtat agaaatacag
      721 gttgttgatg atcaattcag aactccaacc cttgcggagg atttagcgga tggatgctac
      781 ctcattgcaa ccaaacaggc tgccggtatc tggaatatca gcggcaaaga tttactgaca
      841 ccctatcaga tggcaatcaa gactgcggaa tttttcaatc ttgaccaaaa tttgataaaa
      901 agaacaaact cttccgcttt caggcaaagg gcggtacggc cactaaaaac aggtcttgta
      961 atagaaaaag cgcaatctca gttaggttac aaccctcatt cttttgaaga aggaattcaa
     1021 attgtagcgg agcaaatgag tcaaccaaat tgagtgaatt aaccgattaa ataaaccaaa
     1081 ttttgatttc tgagtacatt ttatcatatt cataaaattt tagaaaaacc aaatttttaa
     1141 aactatgtca acgtcaccaa gttcagggga taggggtcag tggggatcaa gttttggatt
     1201 tatcatggct gcggcgggtt cagcagtagg attaggaaat atttggagat ttccctacat
     1261 aaccggacaa aatggaggcg gagcgtttgt atttgtatac atcctctgtg tattgttaat
     1321 cggattgcct ttaatatata atgaaattgc cttgggtaga attacgggta agagcacaat
     1381 aggtgcattc agagataccg gcagtaataa gttcttcatg atatttggtg caattctggc
     1441 attgatggtc agcttctttg tattgagtta ctacggagta atcgccggct ggaccattgg
     1501 atatatagta agtgaattta ctcatttcat aaaagacttt gatacattca gatcttcatc
     1561 cgctttagtt ctgcctttct ttggtttgtt tatggtacta accattggaa tcgttctggg
     1621 cggagtttcc ggaggaatag aaaaagcttc aaaaatatta atgccggtgc ttttcatcct
     1681 gctaattggg gtaatgattc gcagtttgac tttacctggt gccggagaag gagttcggta
     1741 ctatttatat cccgatttca gtaaaattac cggaagagtc gttcttgcag ctttgggaca
     1801 ggcgttcttt tccctttcag taggatgggg aattctgatt acgtatggct cctatcttcc
     1861 aaaaagtcaa aatattgtaa aatccggaat gtggattgga cttatggata ccggggtagc
     1921 gttattagcc ggattaatga ttttccccgc tgtgttcgcg ttctcaaaag atcctgcaag
     1981 tggaacaacc ctggtatttc aggttcttcc ggaaattttt gactctatgc ctgccggtgg
     2041 aaatgttgtt ggtgcattat tctttctgtt gttatgtatc gccgccctga cttccgcgat
     2101 ttccatgctg gaagttcctg cttcttacct gatagatgaa agtaagtgga gccgtaaaaa
     2161 gtcggcttgg acggtaggaa tacttgcctt tttagttggg attccaagcg cgctttcggg
     2221 aggtgcttcc catttgttta ccgaacttac aattccatac ttcggtggaa ccgtaaaaac
     2281 aggtttcctg gatattatgg atgctatctt tggtgagcta ttcattgttg ttgtagcact
     2341 catgaccagt gtttatgcag gatggtttac caataccaga aaacttgctg atgagattgc
     2401 ccatggatca aagttattta ataaacggat tgcaggaagt ttaacctggt caggcttgtg
     2461 ggtattcttt attaaatatg tatgtccgat agtaataggg cttgttcttc taagcacaac
     2521 cggaattatt tagctagttt gcgccggcgg aattttccaa acatagatat ttataatagt
     2581 cctccctgga aacagggctt ctccatatta gttctccctt gagatcaaaa tatgcaacga
     2641 tgtggagatc cgtttctgcc aggaattttc cacagacaaa aattccttca ttttcggttc
     2701 gcaagtaatt aatttcacta aaaactgccg gaatgatgtc tccaattccc gtagaagcaa
     2761 ctcctgagcc tcgtaaagtg ctgtaaataa taacctcctt agcattaggt aattttactc
     2821 tcttgagatt aagcagcgaa attgagtcat cattttgtag atttttcaat tgaaaattgt
     2881 ttcccttgct tacgagcgct tgcgtattgg accaaaattc aatttttgta taatcaaatg
     2941 gaagaattat tttgttgttt acattgatga ttcctgattt accgttctta ctggcgataa
     3001 caagggagtc cccataaggt tgaggccggg tgtcaaattg tggctggata actttccttg
     3061 aaggataatg aaatactcca aatttcccct tcttaagtgt cgcgacaaaa ccattattat
     3121 aggttccaat gccctcatat tcaataggta aaacattttg acccgtggag tcacttaacc
     3181 cggataattt cttttcttca actaaaaaca gaaagtcatt aatgggtttg acttcatcaa
     3241 accatcctgc caaaatcttt ttcccctcag aattatacaa acgtttaact ccatccttgt
     3301 caaatgtcag gagaaaactc ttaccaagtg gtgcctgttg tttaagcaat ttgtagtttc
     3361 ctgaaattga gaagtctaaa agttggttat tctcaaaatg agcaagattt ttcttttcgg
     3421 agaaggagac atagatgtta tcgcttagaa tttttaaaga atcgtattcc gtattcttag
     3481 attcggcagt ttcaaatatg cgccacctgg cattgcgttt atatccgagc tttgatccat
     3541 aaatagaata gttttgaatt gaatcaacaa gaatttttcc tgaatgggaa aataattttg
     3601 ttgtgacttc accttttgaa atccaataat ttttaaagtg actaatctcc tgagttgcgg
     3661 caggaattat ttctttaaga ttctcgtcca gtaaaacttc ctgattagcg ctaaaagata
     3721 agaattcacc gccagttaat tgttccacat cctcaaaagg gaattggaaa gactgcgtgt
     3781 tggtttggtt gtaaagctcc tcgatattgt aaagcccaag ctttccgttt ttttccaggg
     3841 cggcaatctt gccatatcca gtaattgaag tcaggttatc gctaatgatg attttctgga
     3901 atggatccat caaaccccat ccttcagctc ttctaaactt gaaaatggag tttgataaaa
     3961 gttcaatatc ccttaaggag ggctcaaaaa agacataccc atttttgtgg attaatccct
     4021 gtttgccctg tacggaaatc cgaataaatc catttcccaa atctttaacg ctatctgcct
     4081 gagtttgaaa aacaagatcc ttactcttgt tgaacaggct gatttggctc ccgt
//
LOCUS       DASWHX010000037        14212 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_3350704, whole genome shotgun sequence.
ACCESSION   DASWHX010000037 DASWHX010000000
VERSION     DASWHX010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 14212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14212
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3350704"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /locus_tag="VGA21_02100"
     CDS             <1..244
                     /locus_tag="VGA21_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019596524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9649327.1"
                     /translation="NEKGKFVIEVGDNGIGITPEHQKKVFHKFFRVPTGNIHDVKGFG
                     LGLNYVRIMVKAHEGEIKLQSEVNKGTRLKLSFPLA"
     gene            241..927
                     /locus_tag="VGA21_02105"
     CDS             241..927
                     /locus_tag="VGA21_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009581709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX9649328.1"
                     /translation="MKSKAILLVEDDINLGYVIKDNLSINGYHVHHVEDGEKGWQAFQ
                     DSKYELCILDVMLPRKDGFSLAEQIREVNKDIPILFLTAKSMKEDKITGFLKGGDDYI
                     TKPFSIEELLLRINVFMKRSQIFAEGNSTTYNIGSTEFDYKNLTVTIGDEVRTLTQKE
                     AEVLRYLISRPNSVIKRSEILENIWGEDDYFLGRSLDVFISRLRKYIKSDHTLEIVNL
                     HGVGFKLKTK"
     gene            1088..2143
                     /locus_tag="VGA21_02110"
     CDS             1088..2143
                     /locus_tag="VGA21_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009581707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA0069 family radical SAM protein"
                     /protein_id="HEX9649329.1"
                     /translation="MKSWKGRGAQIDPRNPFLKREVVSEHPEGIDEELMAERPPQEIF
                     YENPRKVINKIESPDLRLGFSINPYQGCEHGCVYCYARNVHTYWGFSAGLDFERKIIV
                     KKNAPQILASEFQKRSWKPAPVMLSGNTDCYQPLERKFKITRGLLETFLRYRNPVGMI
                     TKNQLILRDLDILKELSAMNLVHVFISITTLQEDLKRILEPRTSSIKRRFETVQQLSE
                     AGIPVGIMMAPIIPSLNDHEIPGILLKAAEYKAYTASYTVVRLNGQVGEIFRNWIFNT
                     LPDRADKVWKQIESLHGGKVNDSKFGRRMKGDGNYSKHINLLFHTSKRKFLKEIKMPD
                     LDCSRFIRPGETLALFN"
     gene            complement(2140..5214)
                     /locus_tag="VGA21_02115"
     CDS             complement(2140..5214)
                     /locus_tag="VGA21_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009577491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX9649330.1"
                     /translation="MSEELLKAIIQLFAIVAKERITEDERTNIREFLAAHLNHDTIQY
                     HMKLFDEFAQYKVHAGVELTDTDEETAEFVGDWSKITEINKQINKGLTQQQKLFLIVK
                     MIELILADGEISERQSNLIFYIGEMIRIRQKDIDDIINFVKAEEVEDLNYPNVLVIDD
                     GTGKHKWKSKRISSHDLTGVVFFLRIEATETYFMKYLGISDLLLNGSLVKPRRIYVFP
                     TGSNLRGSKVHPVYYSDVVSKFLYHEAKDQISFIAENIWFKFKTGDIGLQGINIAERG
                     GKLIGLMGASGSGKSTLLHVLNGSETPTRGRVLINEVNVHRHKERVEGVIGFVPQDDF
                     LVEELTVYQNMYYAAKLCFSQYKRENLENLINKTLKSLGLFEIKDLKVGAPLDKTISG
                     GQRKRLNIGLELLREPNVLFVDEPTSGLSSRDSENIMDLLKELSLRGKIIFVVIHQPS
                     SDIFKMFDSLIILDVGGYQVFYGNPVDASIYFKNQVNMVNKDQGSCPECGNINPEQIF
                     NIIESRVVNEYGRFTEKRKIPPLQWHETFKEKIVLPQVEIVKGVLRNSLKIPGLIKQA
                     AIFAERDVKSKISDKQYLVINLLEAPVLASFLAFLVKYYATIDVTSAHYFFRVNDNIP
                     IFFFMSIIVSLFMGLTVSAEEIIKDKKILKRESFLHLSKASYLSSKIFILFAISAIQT
                     LSFVLIGVWILEMKGMTFAYWMALFSTSCFANMLGLNISASFKSAVTIYILIPLLLIP
                     QLVLSGVVVKFDKFNPRLTPMDKVPLLGELMASRWAFEALAVTQFSDNDYNEIFFEVE
                     KRRWNSHYMSSYYVPKLESHNTEAYRHYLDPNHDSVIFAYHLSVLANELPWQLEYVGQ
                     HNAINLRRFRVSRFDSVGYNQTVNFLNQLRIMYTRRQNTAILERDSIVNQLTNTPEKE
                     AQYRQLQDEYANEEMNNLVMNRGITVIVEKDGRLIRKAYPIYKDPEPANFFDFREQFF
                     LAKKHFAAMNLDTFYFNLSIIWLMSILLYITLYYDLLKIVVRFISRTPGGEN"
     gene            complement(5382..5684)
                     /locus_tag="VGA21_02120"
     CDS             complement(5382..5684)
                     /locus_tag="VGA21_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014019605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HEX9649331.1"
                     /translation="MGKLANVHPGEILNYEFLEPLEITAYRLSKDLKIPQTRISEIVK
                     GNRRITADTALRLSKYFGNSAKFWLGLQDDFDLEEEKELKETELNEISQYGNKNVT"
     gene            complement(5697..5978)
                     /locus_tag="VGA21_02125"
     CDS             complement(5697..5978)
                     /locus_tag="VGA21_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019670432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEX9649332.1"
                     /translation="MIISFGSKEAEQIWNGIRVKKMPIEIQNVGRRKLRMLNNSQAIA
                     DLRIPPLNRLEKLTGKLSEFYSIRINKQWRIIFIWDKGNASDVEIIDYH"
     gene            complement(6210..7556)
                     /locus_tag="VGA21_02130"
     CDS             complement(6210..7556)
                     /locus_tag="VGA21_02130"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEX9649333.1"
                     /translation="MNILLYRFFRIIFRIALRFFFRKMEIKGKEHCPATGPVIVVANH
                     PATFFDPIVIAMLLKTPLFFLAKAVIFKSRFAAAFFRFFNVIPIHRAQDDPASVGKNE
                     ETFKECYQHLKQNGALMVFPEGISQSVRQLLKIKTGTARIALGAEFQNDFNLGVKILP
                     IGLNYSNQEKFQSNLFISIGEPIPVKNFEAIYRNDEREAVSQLTKLIENKIEEQIINA
                     GDKNLDKLIVNIEKLMKNRILMEWGVSKNDPEFDHEAAKRIAEAVHYFRDHDPDRFSK
                     IEKHINNYFEAIKDVDLPENIFESKRLSLKAFFLYTTYFLSGLPVFLFGFLNNIIPYQ
                     ISILVAQMSSGLKEYRGAIGFTAGTFIFLFFYGSGFYLIFHLTHIFMLALIYVLLAPV
                     SGIFAFYYRKKLLRFYYKVVFTFSRKKYLIQHLISLRNNIISQLETARKVFLQANS"
     gene            7888..8133
                     /locus_tag="VGA21_02135"
     CDS             7888..8133
                     /locus_tag="VGA21_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020532185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEX9649334.1"
                     /translation="MNIFVAKLNYKTSSDTLRAAFEAFGEVSSSKIIRDRETGRSKGF
                     GFVEMPDDTAGMEAIQKLNDTELDGRQIVVKQANPKD"
     gene            complement(8293..8424)
                     /locus_tag="VGA21_02140"
     CDS             complement(8293..8424)
                     /locus_tag="VGA21_02140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649335.1"
                     /translation="MLELIYNEESPQKQILEKGYNFIIAGKDNLAVGFTSYELNYNE"
     gene            complement(8519..8674)
                     /locus_tag="VGA21_02145"
     CDS             complement(8519..8674)
                     /locus_tag="VGA21_02145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649336.1"
                     /translation="MSKPELRKNFTVFILFFGMALIEAPQSANWLKAVFWVGTAQYYL
                     GKIAYHT"
     gene            complement(8713..9414)
                     /locus_tag="VGA21_02150"
     CDS             complement(8713..9414)
                     /locus_tag="VGA21_02150"
                     /inference="COORDINATES: protein motif:HMM:NF025687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 domain-containing protein"
                     /protein_id="HEX9649337.1"
                     /translation="MDNNQLKRTARLAGFLFFLWIVTGFYDMFFVSPKIFVAGDHVAS
                     AQNILTHELLFRSSIFSGLITSTIWIFLVWVFYQLFKSVNEHYAKLLVAFVIAQVPVA
                     FIKGGFSIATLMILKGEVLTSFEVLQRQDLAMLFLKINDYSVLALELFWGLWLFPLAI
                     LIYQSNFIPRLLGIWLIVNGTVYVLLSFTSIVFPLYKDLVFTVGMPAMFGELVLMLWL
                     LIKGIRVREEVAKPT"
     gene            complement(9437..10171)
                     /locus_tag="VGA21_02155"
     CDS             complement(9437..10171)
                     /locus_tag="VGA21_02155"
                     /inference="COORDINATES: protein motif:HMM:NF025687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 domain-containing protein"
                     /protein_id="HEX9649338.1"
                     /translation="MNSTNIRSEVSPQIYARIGGVLYLLIIGLGIFYEMIVRDSIVIP
                     GDAMATMASLKENEFFWRLGIIAEFLSSIIVFGLILIMYKLTKPVNKDLAMLAAFFNL
                     SAATIQTVYIIQLVEVLFPIGAPAYLQAFTTEQLGAMVSLSMKSHVFGFGIALLMFGP
                     YFLITGFLIYKSNYLPKFIGILYILSGVGYLVNSVMLILAPQFSGIVFMIVMPPVFIG
                     EMSLALTMLIKGVNETEWNKYASNES"
     gene            complement(10242..12848)
                     /locus_tag="VGA21_02160"
     CDS             complement(10242..12848)
                     /locus_tag="VGA21_02160"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9649339.1"
                     /translation="MNNAHSFHPPQWPVKFLRFFLKEKYFEEIEGDMEEIFYENSERF
                     SVRKAKRMYTWEMIKLLRPNLIKNLEFINYLNQYSMFKNYFKVSFRGLMKNPVNSFIN
                     VFGLAIAIGLCVFAYAFARWTFSTDQFHENKHSVYLTTFYANRDGVNQQYGTTPRPIG
                     EMLRDDFAQIKTVCRIEDRSVVMKYDDNVFHERVRYVDPEFLEMFTFPLKWGTARSLK
                     DFNSIILSEPMSVKYFGDENPIGQSILMIYGKEQSKAFKVAGVAAEFPKARTISFDFL
                     INFENFRTTDPGYNYYDWNAFVNATFIQVDKPSDLVSIKSKMEKYKSLQNDVVKEDWA
                     ISSFSFEPLATLHEQSEYIKDDISRSSKSNYVSIYFMVVIAVFMLALACVNYINIAIA
                     TAAKRLKEIGVRKSIGATRKVVIVQFLTENMVLTSFTLIIGVALGYFFFIPGFESLWH
                     FNMDFRFTDVNLWIYLPIILLVTSIASGIYPAFYISRFHAVTILKGSVKFGQRNLLTK
                     VFLCFQLIFACVFITMSVMFTQNTDYMSKRSWGYNPADALYAKVPDQSSFEKLSAIIA
                     QNPDVLSISGSTHHVGKNHKSTVLHFPDRNYEVDQLSVDSKYFETIGLELKEGRVFHD
                     FEGSDRQAVIINETLAKNIGENSIGQVFQIDTIQYEVIGVLKEFHSYSFSQTVRPIIF
                     NVADKADYRFLSLKARSGSEIQLYKSLQAGWSELFPEIPFDGGLQQDVWGFYYQEIGI
                     YKLVWKVFAFLAITLATLGLYGLVRLNVEGRIKEFSIRKVLGAGLKNISANVTNQYLV
                     LFLLALIMGAPLGQWLGTWMITFSNEYHMPITFSGTIISVMITTLVLLATVATQIWRI
                     MKSKPVIGLKME"
     gene            complement(12845..13183)
                     /locus_tag="VGA21_02165"
     CDS             complement(12845..13183)
                     /locus_tag="VGA21_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020533265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX9649340.1"
                     /translation="MKKTKLGEFEELVLLTVAVLQQDAYGVEIKRELEERLKHKLSVG
                     SIQSSLKRMEEKGFLTSQFGEATQKRGGKRKRIYATTSHGRKVLQEMKDIRAGLWSAI
                     PRLAYELKVI"
     gene            complement(13203..13937)
                     /locus_tag="VGA21_02170"
     CDS             complement(13203..13937)
                     /locus_tag="VGA21_02170"
                     /inference="COORDINATES: protein motif:HMM:NF025687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 domain-containing protein"
                     /protein_id="HEX9649341.1"
                     /translation="MEAILESNLSARQASKTARLAAALFFFLCVPLALWDQFYVPQKI
                     FVAQDPVATANNLLSNEFIFRTSIVTHLAGFLIFVMMVLLFHRIFRPVDKHLSRLMIF
                     SVLAQVPVVFVFEVFNFTALMILKSEPRLTFDVAQQQEAAYFLMRMPRYAVGAGMGKL
                     FLGLCFIPFGMLVFRSGFAPRIIGILLIIGGVGYVADCCISILLQRTDYVMVRSFLMY
                     TTVGYALALLWFLIKGVQEKKAVVHQ"
BASE COUNT         4393 a         2881 c         2766 g         4172 t
ORIGIN      
        1 aaatgaaaaa gggaagtttg ttattgaagt cggagacaat ggaataggaa ttacacccga
       61 acatcaaaag aaagtatttc acaaattctt cagggttcct accggaaata ttcatgatgt
      121 aaaaggattt ggcctgggtt tgaattacgt gcggataatg gtgaaagcgc atgagggaga
      181 aattaaatta cagagcgaag tcaacaaagg aaccagactt aaactctcat ttccattggc
      241 atgaaatcaa aggcaatttt attggttgaa gatgatatta acctggggta tgtaataaaa
      301 gataatctgt caataaacgg gtaccatgta caccatgttg aagatggtga aaaaggctgg
      361 caggcatttc aggactccaa atacgagctt tgcattttag atgtgatgct gcccaggaaa
      421 gacggatttt cactggctga acaaatccgg gaagtgaaca aagatattcc aatcctgttt
      481 cttactgcca agtcgatgaa ggaggataaa ataaccggct tcctgaaagg aggagatgat
      541 tacataacca aacccttcag cattgaagag cttttgttac gcattaatgt tttcatgaaa
      601 agatctcaaa tattcgcgga agggaattca actacttaca atattggcag tacagaattc
      661 gattataaaa acctgaccgt taccattggg gatgaagtga gaactcttac gcaaaaggaa
      721 gcggaggtat tgcggtatct gatcagcaga cccaacagcg taatcaaacg cagcgagatc
      781 ctggaaaata tttggggtga agatgattat ttcctgggtc gaagccttga cgtgtttatc
      841 tcgaggctgc gaaaatacat caaatcagac cataccctgg aaattgtgaa tctgcatggt
      901 gttggcttta agctcaaaac gaaataaggt ttacttccag atacgtgtgt cacacttttt
      961 cgacatagaa aagcaatcaa atttcaccta aacataaaga cggaagtctg ccacacgtag
     1021 atcctcccgc taacaataat actaactgaa ttagctatta tactaattat aatagtatat
     1081 tgtgttcatg aaatcctgga aaggcagggg ggcgcagata gatcctagaa atccctttct
     1141 taaaagggag gttgtttcgg agcatcccga aggaattgat gaggagttaa tggctgagag
     1201 gcctccgcaa gaaatattct atgaaaatcc aaggaaagta attaacaaaa tagagagtcc
     1261 cgatttgcgc ctcggatttt caatcaatcc gtaccaggga tgtgaacatg gctgcgttta
     1321 ttgttatgcg aggaacgtgc atacttattg gggatttagc gcgggtcttg attttgaacg
     1381 gaaaataatt gtgaagaaaa atgctcctca gattctggca tcagaatttc aaaaacgcag
     1441 ctggaaaccg gcgccggtga tgctttccgg caacaccgat tgttatcagc cgttggaaag
     1501 aaaattcaaa ataaccaggg gtttactgga aacattcctt cgttaccgca accccgttgg
     1561 tatgattaca aaaaatcaat taattcttcg cgatcttgat atcctgaaag aactgtcggc
     1621 aatgaacctg gttcatgtgt ttattagtat aactacactg caggaagatt tgaaaagaat
     1681 tcttgaacca agaacctcca gcataaaaag aaggtttgaa acggtgcagc aactgtcaga
     1741 agccggaatt cctgttggca ttatgatggc tccaattatt ccatcattaa atgatcatga
     1801 aataccaggc atcctcctga aagctgccga gtacaaagca tatacagcca gttatacggt
     1861 agtccggtta aatgggcaag taggtgaaat tttccgtaac tggattttta acactttgcc
     1921 tgatcgtgcc gacaaagttt ggaaacaaat tgaatcgttg catggtggca aggttaacga
     1981 ttcaaaattt ggaagaagaa tgaagggtga cggaaactat tcgaaacaca tcaatctgct
     2041 tttccatact tctaaaagaa aatttctgaa agaaataaaa atgccggacc tggattgcag
     2101 cagatttatc aggcccggtg aaacacttgc cttattcaat taattttctc cgccaggagt
     2161 tctgctgata aagcgtacca ctatctttag caagtcataa taaagagtaa tgtaaaggag
     2221 gatactcatt aaccagatta tggaaaggtt gaaataaaaa gtatctaagt tcatggccgc
     2281 aaaatgtttt ttggctaaaa aaaactgttc ccgaaagtca aagaaatttg ccggctcggg
     2341 atctttataa atagggtaag ctttacgaat taatcttcca tctttttcaa caataacggt
     2401 aataccacga ttcatcacca ggttattcat ttcttcattg gcgtattcat cctgaagttg
     2461 gcgatattga gcctcctttt ccggagtatt agtaagctga tttactattg aatctctttc
     2521 taaaatggca gtattctgtc tcctggtata cataattctt aactggttca ggaaattgac
     2581 ggtttggtta taacctaccg aatcaaatct tgaaactctg aaccggcgaa gatttatggc
     2641 attatgttgc cccacgtatt ccaactgcca cggcaattca tttgcaagta cagaaaggtg
     2701 ataggcaaaa atcacactat cgtgattcgg atccagataa tgtctgtacg cttccgtatt
     2761 gtgtgactcc aatttcggca cataataact gctcatataa tgtgaattcc atcgcctttt
     2821 ttccacctca aagaatattt cattataatc attatcactg aattgggtaa cggctaaagc
     2881 ctcaaaggcc catcggcttg ccattagttc tcccaaaagc ggaactttat ccattggagt
     2941 gagcctggga ttgaacttgt cgaattttac cacaacgcca ctaagaacaa gctgaggtat
     3001 taacaagagc ggaataagaa tgtaaatagt tacggccgat ttaaagctgg cagaaatatt
     3061 taaacccagc atgttggcaa aacaggaggt tgaaaataat gccatccagt aggcaaaagt
     3121 catccctttc atttccagaa tccatacccc gattaaaacg aaactgagag tttggatggc
     3181 tgaaattgca aacaagataa aaatcttaga tgataagtaa cttgccttgc tgagatgaag
     3241 aaatgattcc cgcttcagaa tttttttatc cttgattatc tcctccgcac ttaccgttaa
     3301 tcccataaat aatgaaacaa taatgctcat aaaaaagaat atgggaatgt tatcattaac
     3361 gcggaaaaaa taatgggcac tggtgacatc aatggttgca taatacttaa ccaggaacgc
     3421 gagaaatgaa gcaagaacag gagcttccag taaatttatt accaggtatt gcttatcact
     3481 gattttgctc tttacatccc gctccgcgaa gattgccgct tgctttatca ggcccggtat
     3541 ttttaatgag tttcttaaaa cgccttttac aatttctacc tgcggtaaga ctatcttttc
     3601 tttgaaagtt tcatgccatt gcaaaggggg tatttttctc ttttcggtaa atcgtccata
     3661 ttcatttaca acccggcttt caataatatt gaaaatctgt tcaggattta tattgccgca
     3721 ttccgggcag gagccctggt ctttatttac catgtttacc tggttcttga agtaaatgct
     3781 ggcgtcaaca gggtttccat aaaatacctg gtagcctccc acatccagaa taattaaact
     3841 gtcgaacatt ttgaagatgt cggatgaagg ctgatggatt accacaaaaa taattttacc
     3901 ccgaagggat aattctttaa ggagatccat gatgttctca gaatcccggc ttgaaagccc
     3961 ggaggtgggc tcatcaacaa aaagtacgtt tggttcgcgc agcagttcaa gaccgatgtt
     4021 caaccttttc ctctggccgc cgctaatggt tttgtctaat ggagcaccaa ctttcaaatc
     4081 tttgatttca aataatccaa gacttttaag cgtcttattt atcagatttt ctaaattctc
     4141 tcttttatac tgactaaaac atagtttagc cgcgtaatac atgttttggt aaacggtaag
     4201 ctcttccacc aggaagtcat cctggggaac aaatccaatg acgccctcca ctcgttcttt
     4261 atggcggtgg acatttactt cattaatgag aaccctgcca cgggttggag tttctgatcc
     4321 atttagtaca tgaagcagcg tagatttgcc cgagccgctg gcgcccatga gaccaattag
     4381 ctttccgccc ctttcggcaa tattaatgcc ctggagacct atatctcctg ttttgaactt
     4441 aaaccagata ttttctgcaa taaaagaaat ttgatctttg gcttcatggt aaagaaactt
     4501 actaactaca tcactataat acacagggtg aacctttgat ccacgcagat tgcttccggt
     4561 tgggaataca taaattctgc ggggcttaac cagacttcca tttagcagta aatccgagat
     4621 tcccaggtat ttcatgaaat aggtttctgt tgcttctatc cgtaaaaaga aaacaactcc
     4681 ggtcaggtca tgagatgaga tgcgtttgct tttccactta tgttttcctg ttccgtcatc
     4741 aataacgagt acgtttggat aattcagatc ttcaacttct tcagctttta caaaattgat
     4801 gatgtcatca atatctttct ggcgaattct aatcatttca ccaatgtaaa aaatcaggtt
     4861 actctgacgc tcagagatct cgccatcagc cagaattaac tcaatcatct ttacgattaa
     4921 gaataacttc tgctgttgag ttaatccctt attaatttgt ttattgattt cagtaatctt
     4981 cgaccagtct ccgacaaatt ccgctgtttc ttcatcggta tcggtgagtt ccacgccggc
     5041 atggacttta tactgcgcga actcatcgaa aagcttcatg tgatactgga tggtatcatg
     5101 gttaaggtgg gcggccaaaa actcccggat atttgtccgc tcatcttcag tgatccgctc
     5161 cttggctaca atggcaaata gctgaataat tgctttaagt aattcctcgc tcatttagaa
     5221 tcctgctggt atacttcgaa aatagacaaa ctcattgcga aattagcaaa ataaataccg
     5281 attccgattc aaataatcct taagtaggct ggttagaata agcaggaaag gtttcccttt
     5341 atagttggac tggtttttca cacaatataa tacaccggtt gttaggtaac gtttttattt
     5401 ccgtattgac ttatttcgtt cagctccgtt tctttcaatt ctttttcttc ttcaaggtca
     5461 aaatcgtctt gaagtccgag ccagaatttt gcagaattcc cgaaatattt gctcaatcgc
     5521 aaagctgtgt cagctgtaat tcttcgattt ccttttacaa tttcagaaat tctagtctgc
     5581 ggtattttta aatcttttga caatctgtac gcagtaattt ccaatggctc aagaaattcg
     5641 taattcagga tttctccagg atgtacattt gctagctttc ccatttgttt ctttttttaa
     5701 tgataatcaa ttatttccac gtcacttgca tttcctttgt cccaaatgaa gataattctc
     5761 cattgtttat taatccgaat gctgtaaaat tcactcaatt ttccagtcag tttttcaagt
     5821 cggtttaatg gtggaattct caaatcggca atggcttgcg agttgttcag cattctcaat
     5881 ttcctgcgtc ctacgttttg aatttcaatc ggcatttttt taactcggat tccgttccaa
     5941 atctgttcag cttctttcga gccaaatgaa ataatcatat ttctgtttta cgttactaac
     6001 gtcaaaggta ggtataaagt ttgatttttc ccaatgttgc ctaacggccc gatacatcga
     6061 cttaaactat ttttagtgac aagtgtggca catgctgatg ttaatttcat ttggatttaa
     6121 attgaggcac taccaaattt ttaccattac cttgaaaata atagaaggtt ttcaggacaa
     6181 ggaataatcc gatccttcat tcagagttat caactgttag cttgcaaaaa tactttccgc
     6241 gcagtttcca gttgtgaaat tatattattt cttaaggaaa ttaagtgctg aatcaaatac
     6301 tttttcctgg aaaatgtgaa taccacttta taataaaaac gaagaagttt cttgcgataa
     6361 taaaaagcaa aaataccgga taccggggcc agcaaaacat aaataagtgc gagcataaag
     6421 atatgggtta agtgaaaaat caggtaaaat ccggaaccgt agaagaagag gaaaataaaa
     6481 gttccggcgg taaatccgat tgcacccctg tactctttta acccggatga catttgggca
     6541 acaagaatag aaatttggta aggtattata ttattcagaa aaccaaagag gaaaaccggc
     6601 agaccagaaa gaaaataagt agtgtacaga aaaaaagctt tgagacttaa tcgtttactt
     6661 tcaaaaatat tttcgggaag gtctacgtct ttgatggctt caaaatagtt gttgatatgt
     6721 ttttcaattt ttgaaaacct gtccggatca tgatctctga aataatgcac agcttcagct
     6781 atccgttttg cagcttcatg atcaaattca ggatcattct ttgacacacc ccattccata
     6841 agaatgcggt tcttcattaa cttttcaata tttacaatca gtttatccaa attcttgtct
     6901 cccgcattaa taatttgttc ttcaatctta ttttcaatca atttcgttaa ttgacttacc
     6961 gcttcgcgct catcatttcg gtaaattgcc tcaaagttct taaccggtat gggctctccg
     7021 atggagataa acaagttgga ttgaaatttt tcctggttgg agtaattcaa tccaatcgga
     7081 agtattttta ctccaaggtt aaagtcattt tgaaattccg cgcctaacgc aattcttgct
     7141 gttccggttt taatcttgag tagttgccgc actgattgcg aaatcccttc cgggaaaacc
     7201 atcaaagcac cgttctgttt caaatgctgg tagcattctt taaatgtttc ttcatttttc
     7261 cctacggaag ccggatcatc ctgggccctg tgaatgggaa taacgttaaa aaatcgaaaa
     7321 aatgcggccg caaacctgct tttaaatata acagccttgg caagaaaaaa tagcggagtt
     7381 tttaacagca tcgcgataac aattggatca aaaaaagtag cgggatggtt agcgacgact
     7441 atcaccggac cggttgccgg gcaatgttct tttcctttta tttccatttt tcggaaaaag
     7501 aacctcaggg caattctaaa tattatcctg aagaacctgt ataaaagaat gttcatgccc
     7561 tgaaatttat gaatttgaaa tgtgttaacg caatgcagta aggattgatg gcggtaaata
     7621 ccttcaccgt caattattaa tatctttgaa attcaatagt tgaagcctaa aaaatgccgg
     7681 aatagggcaa ttttcttgca ttaaaccaaa gattatgggt acgcaatatt cgataactcc
     7741 atctgatttt atttgcgtaa atgtatttga aagtttaatt tccttatatt tttgtataga
     7801 atctgcaaaa gttaattcag gctaattccc ttagtaatta ttctggcttc ttttaacgat
     7861 cgccttttta aacttaattt ttttacgatg aacatttttg ttgccaaatt aaactacaag
     7921 acttccagtg acactcttag agctgcattc gaagcatttg gtgaggtatc ctcatcaaag
     7981 atcatcagag atcgggaaac aggacgatca aaaggtttcg ggtttgttga aatgccggat
     8041 gatacagccg gaatggaagc aattcaaaaa ttaaatgaca cagaactgga tggaagacaa
     8101 attgtagtta aacaagcgaa tccaaaggac taaaagatat aatcaatcta cctaaaggaa
     8161 tatgaaggct atctcattaa cagatagcct tttttgtatg caattattcc aattttcttc
     8221 tcagaaatgc ttgcccttga aaaataataa tgccctggga tgaagctaaa ttctatgtat
     8281 catcagttgg gactattcat tatagtttaa ctcataggaa gtaaacccaa ctgctaaatt
     8341 gtcttttcct gcaattatga aattataacc tttttcaagt atttgtttct gtggcgattc
     8401 ttcattgtag attaattcaa gcatatactc aatttgttcc tttggcaaaa ctttcccgaa
     8461 tgtgacaggt caggtctcgt agacaatttg cctgactttg ggcaattcat catatttttt
     8521 aagtatggta agctattttg ccaagataat actgtgcagt acccacccag aaaacagcct
     8581 taagccagtt ggctgactgg ggtgcctcaa tgagtgccat accgaaaaaa agaataaaga
     8641 cagtaaagtt ttttctgagt tctggcttac tcattttatg cattgactta atgagcaatc
     8701 agtctaattc aattaagttg gtttagctac ctcctctctc acgcgaatgc ccttaatcaa
     8761 taaccataac attaagacaa gttcaccaaa catggcaggc atgcccactg taaaaaccaa
     8821 gtccttgtac agcgggaata caatacttgt aaaacttaat agaacataca cagttccgtt
     8881 aacaattaac caaataccta acaaacgtgg gatgaaatta gactggtata tcaggatggc
     8941 taaaggaaag agccacaacc cccaaaataa ttccaacgcc aacacactat aatcattgat
     9001 tttgagaaac aacatggcta agtcttgtcg ttggagcact tcaaaggatg ttaaaacttc
     9061 accctttaat atcattagcg tagctatact aaatccacct ttaataaaag ccacgggcac
     9121 ctgtgcaatt acaaatgcaa ccagaagttt ggcatagtgc tcattaacgg atttgaacaa
     9181 ttgataaaag acccaaacga gaaaaatcca aatggtgctg gtaattaatc ctgaaaatat
     9241 gctcgagcga aataacagct cgtgtgtgag aatattttga gcagaggcaa cgtgatcacc
     9301 tgctacaaaa atcttcggtg atacaaaaaa catgtcgtag aatcccgtta ctatccatag
     9361 aaagaacaag aagcccgcca aacgggccgt tcttttgagt tgattattgt ccataatgct
     9421 tagtgtttat ggcttattaa ctttcattgg atgcatactt attccattca gtttcattta
     9481 ctcctttaat taacatcgtt agcgctaacg acatttctcc aataaagacg ggcggcataa
     9541 caatcatgaa tacaatcccg gaaaactgcg gtgctaatat gagcatgacg ctattaacca
     9601 gataccccac accagaaagg atgtacagaa tgccaatgaa tttcgggagg tagtttgact
     9661 tgtatattaa aaagccggta atgagaaaat aaggaccaaa cattaacaag gctattccaa
     9721 atccaaatac atgactcttc atgctaaggc ttaccatcgc tccaagctgc tcagttgtga
     9781 aagcttgaag atatgcgggt gcacctatcg gaaataacac ctctacaagt tgtatgatat
     9841 atactgtctg aatggtagca gccgacaggt tgaaaaaggc tgccagcata gccaagtctt
     9901 tgttgactgg ctttgttaac ttatacatta tcagaataag gccgaaaacg attatggatg
     9961 atagaaattc agcaatgata ccgagtcgcc agaaaaactc attttcctta aggctcgcca
    10021 tggtagccat agcatcaccc ggaataacta tgctatctct cacaatcatt tcataaaaaa
    10081 tgccaaggcc tatgattaaa agatacaaaa cgccaccaat cctggcataa atctgcggtg
    10141 atacttcact tctgatgttg gttgaattca tgtatttttt ttatagttaa ttattttttt
    10201 ttgagggagc acaaaggttt gattttcctc ttgaaatctt actactccat cttcaatcca
    10261 attacaggtt ttgatttcat tatccgccaa atctgtgtag caacggttgc caataaaaca
    10321 agcgtggtaa tcataaccga gatgatagta ccagagaagg taatcggcat gtgatactca
    10381 ttgctgaaag ttatcatcca ggttccaagc cattgaccaa gtggagcccc catgatcagg
    10441 gcaagcaaga acagaaccag gtattgattg gtcacattag ctgaaatatt tttaagaccg
    10501 gcacccagta cctttctaat actgaattct ttgatgcgcc cttccacatt gagacgcacc
    10561 aggccgtaaa gtccaagcgt tgctaatgta attgccaaaa aagcaaaaac tttccaaacc
    10621 agtttataga ttccaatttc ctggtagtaa aagccccaaa cgtcttgttg aagtccacca
    10681 tcaaatggaa tttcagggaa aagttcggac cagccagctt gaagagattt gtaaagctga
    10741 atctccgatc cacttcgtgc tttcagggag agaaaccgat aatccgcttt gtcggccacg
    10801 ttgaaaatga tggggcgtac ggtttgggag aacgaatagc tatgaaattc tttaagcacg
    10861 ccaatcactt catattgaat ggtgtcgatt tgaaatacct ggccgatgga attttctcca
    10921 atatttttgg ccagtgtttc attgataata acagcctgcc ggtcgctgcc ttcaaagtca
    10981 tgaaatactc gtccctcctt tagctcaagg ccgatcgttt cgaaatattt cgaatcgacc
    11041 gagagttgat cgacttcata atttcgatcc ggaaaatgca gcacagttga cttgtgattt
    11101 ttacccacat gatgagttga accggatatc gataatacgt cagggttttg ggctatgatc
    11161 gcactcagtt tttcaaaaga ggactgatcc ggaaccttag catacagtgc atcagccggg
    11221 ttatagcccc aactgcgctt gctcatgtaa tcggtgttct gcgtgaacat taccgacatg
    11281 gtgatgaaaa cgcaggcaaa tattaattga aaacacaaga acactttcgt cagcaggttt
    11341 cgctgaccaa acttaacact gcccttcaaa attgtaaccg catggaaccg cgaaatgtaa
    11401 aaggccggat aaattccaga ggcgatactg gtgaccagta agataatggg aagatagatc
    11461 cagaggttta catccgtgaa ccggaaatcc atgttgaaat gccagagtga ttcaaatcct
    11521 ggaatgaaaa aaaagtaccc cagtgctact ccaatgatca acgtaaacga agtaagcacc
    11581 atattctccg tgaggaactg cacaataact acttttcggg tagcccctat agattttcgc
    11641 acgccaatct ctttcaatct ttttgcagcc gtggcgatag caatgtttat gtagtttacg
    11701 caggcgagtg ccagcatgaa caccgcgata accaccatga aataaatgga tacataattg
    11761 ctttttgaac ttctggagat atcgtcttta atatattccg actgctcgtg cagtgtggct
    11821 aacggctcga atgagaatga cgaaatagcc caatcttctt tcaccacatc attttgtaac
    11881 gatttatact tttccatttt cgatttgatg gacaccagat cggaaggctt gtcgacttga
    11941 atgaacgtgg cgttgacaaa tgcattccaa tcataatagt tgtaaccagg gtcagtcgtg
    12001 cggaagtttt cgaaattgat caaaaagtca aaactaatag tgcgtgcctt gggaaattct
    12061 gcggctacgc ctgcaacctt gaaagccttg ctttgctcct tcccgtaaat catcaggatg
    12121 ctttgaccga tggggttttc atctccaaag tattttaccg acatcggttc gctcaggatg
    12181 atgctgttaa aatctttcaa tgaccgggct gttccccact tgagcggaaa ggtaaacatt
    12241 tcaagaaact ccgggtcgac ataccgtacg cgttcatgga aaacattgtc gtcatacttc
    12301 atcaccacac tgcggtcttc aattctgcac acggttttga tttgagcgaa atcgtcacgc
    12361 agcatttcac caatcggccg cggtgttgtt ccatactgct ggttcacgcc atcccggtta
    12421 gcataaaaag tggtgagata aacagaatgc ttgttttcat gaaattgatc agtactgaat
    12481 gtccagcgcg caaaagcata tgcaaacaca cacaacccga ttgctatagc cagaccaaat
    12541 acattaatga atgaattgac cgggtttttc attaaacctc taaacgaaac tttaaaatag
    12601 tttttgaaca tgctgtattg atttaaatag ttaataaact ccaggttttt gatcaggttg
    12661 ggtcgtaaga gttttatcat ctcccaggta tacattcttt ttgctttgcg aaccgaaaaa
    12721 cgctcggaat tctcatagaa gatttcttcc atgtcgcctt caatctcctc gaagtacttc
    12781 tccttcaaaa agaaccggag gaatttcaca ggccactgtg gtggatggaa ggaatgagcg
    12841 ttgttcatat caccttcaat tcataagcaa gcctgggaat ggcactccac agaccggctc
    12901 gaatgtcttt catctcttgc agcactttac ggccgtgtga ggtagtggca taaatccgct
    12961 tgcgtttgcc gccacgcttc tgagtggctt ctccaaattg agaagtgaga aaccctttct
    13021 cttccatacg tttgagtgag gattggatgg aaccaacgct taatttatgt ttaagtcgtt
    13081 cttccaattc ccgctttatc tctactccat aggcatcttg ttggagtact gctactgtta
    13141 agagtaccag ttcttcaaat tctccgagtt tggttttctt catattattt atattccagg
    13201 ttttattgat gaacaactgc ctttttctcc tgcacaccct tgatcaggaa ccatagcagg
    13261 gccagggcat atcccaccgt agtgtacatc aggaacgacc gtaccatcac atagtcagtt
    13321 cgctgcagga gaatagagat gcaacagtcc gcgacataac ccactccacc aatgatcaac
    13381 agtatcccaa taatccgcgg tgcgaacccg gatcggaaaa ccagcatccc aaaaggaatg
    13441 aaacacagtc ccaggaagag cttgcccatg ccagccccaa ctgcataccg aggcatgcgc
    13501 atcaagaaat atgcagcctc ctgttgttga gctacatcaa aagtcaaacg gggctcactt
    13561 tttagaatca ttagtgctgt gaagttgaag acttcaaaga caaagacgac cgggacctgg
    13621 gccagtacag agaatatcat aagccgggac agatgtttgt ctaccggcct gaaaatgcgg
    13681 tggaacagca gtaccatcat cacaaaaatt aagaagcctg cgaggtgggt gacgattgag
    13741 gttcgaaaaa tgaattcatt tgaaagcaga ttattagcgg tcgcaacggg atcctgagcc
    13801 acaaagatct tctgggggac atagaactgg tcccagagtg ccagggggac acacagaaag
    13861 aaaaataatg cggcagcaag ccttgctgtt ttgctggcct gcctggcaga caggttcgat
    13921 tccaagattg cttccatatt tataaattaa gacaatgggc tttcaaaagg tccgcagagt
    13981 caatataatg atattaatcg taattgtagt aaaaatagtt acatatttta ttcgtaattg
    14041 tagtaaatat ttatatttgt tcgaaactgt caaaatttcg aagataagtc caatcctata
    14101 actttttaat tatgacctga aaattgaagt tgccgaaccc cttcgctgct tgtgcggaaa
    14161 tattaacgcc tttataagaa ccataatccc aatgtaactt caaaaatact ga
//
LOCUS       DASWHX010000038         2294 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_2244288, whole genome shotgun sequence.
ACCESSION   DASWHX010000038 DASWHX010000000
VERSION     DASWHX010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 2294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2294
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2244288"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..869
                     /gene="dnaX"
                     /locus_tag="VGA21_02175"
     CDS             <1..869
                     /gene="dnaX"
                     /locus_tag="VGA21_02175"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018342565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEX9649342.1"
                     /translation="NSVDDIRNLIDQVRYPPQQGKYKVYIIDEVHMLSSAAFNAFLKT
                     LEEPPSYAIFILATTEKQKVIPTILSRCQIFDFNRIQVSDIADHLKKIAAEENVKAEA
                     EALHLIAQKADGALRDALSIFDLMVTFSSDQSITYQSIIENLHILDYEYYFKVVDYLY
                     LENISDALITLDEIFSLGFDGHNFISGLSEHLRNLLVAREPKTIHLLQVSESSHEKFR
                     KQAGSVSPSWLLSALNITAQAELNYKATKNPRLLVELSLMKIAYLNQALNPREISPDV
                     KKKSLIASQSLN"
     gene            1010..1471
                     /locus_tag="VGA21_02180"
     CDS             1010..1471
                     /locus_tag="VGA21_02180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649343.1"
                     /translation="MKSTTKLPSLKTEEDSSPNETSGQEPANPGKRREEIDPEALKSF
                     WNSFKEKRKELGRDRELNVLNQEFDLKDEEIQLQLLNPIQGDILEDFREELLDYLRSK
                     LKNDFISLKTRMVKQDAKKMIYTSKEKLDHLIQKNPQLKNLKDSLGLDNEY"
     gene            complement(1468..1878)
                     /locus_tag="VGA21_02185"
     CDS             complement(1468..1878)
                     /locus_tag="VGA21_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018344429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9649344.1"
                     /translation="MKADLSIEKVLLVDDNDTDNFISKRIIEITNFAKDVEVKSSGKA
                     ALDFLEENKNDAQYLPDLIFLDINMPIVDGFVFLYEFEKFNALIKNKCKVVILSSSDN
                     KRDIDKIVNNNYVIKFITKPLTETALREIKLMTA"
     gene            complement(2025..>2294)
                     /locus_tag="VGA21_02190"
     CDS             complement(2025..>2294)
                     /locus_tag="VGA21_02190"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEX9649345.1"
                     /translation="YGISIEELRVWNSIEDVNSLKMDQELKIMQEKMKLEKELDKTYT
                     KHEVAAGDTLYRISKLYDVSLNDLLKWNNKNSSELKIGETLLIKK"
BASE COUNT          756 a          442 c          391 g          705 t
ORIGIN      
        1 ataattccgt ggatgatatc cgcaacctga ttgaccaggt acgatatcca cctcaacaag
       61 gcaagtataa ggtttatatt attgatgagg tacatatgct ctccagtgcg gctttcaatg
      121 catttttgaa aaccctcgaa gagccgcctt cctatgcaat ttttattctg gccacaacag
      181 aaaagcagaa agttattcca acaattctct ccagatgtca gatttttgat tttaacagaa
      241 ttcaggtatc cgatattgct gaccatctta agaaaattgc ggcggaggaa aacgtgaaag
      301 ctgaagctga agccctgcat ttaattgcgc aaaaagcgga tggtgctctt cgtgatgcgc
      361 tcagcatctt tgatctgatg gtcactttct cttcagacca gagcattact taccaatcca
      421 ttatagaaaa tctccatatc ctggattatg agtactattt caaagtcgtt gattatctgt
      481 atttggaaaa tatcagtgac gctttgataa ctttagatga aatttttagt ttgggatttg
      541 acggacataa ttttatttcc ggactaagcg aacatttgag gaatctcctg gtagcccgtg
      601 aacctaaaac gattcattta ttgcaagttt ctgaatcttc tcacgaaaaa tttcgaaaac
      661 aagcgggttc tgtttcacct tcctggttat taagtgcctt aaatattact gcgcaggcag
      721 aattgaatta caaggcaacc aagaatccca gattactcgt tgagctttcg ttaatgaaaa
      781 ttgcttacct gaaccaggct ttaaatccca gggaaatctc tccagatgtt aaaaaaaaaa
      841 gcctgatagc aagccagagc ctcaattaga aaatcctttt ccaaaacctg aagaaaaaca
      901 aattcctaca gtcaaaaaag aatctaaccc gactgcttca actaaacatg aaggaatcgt
      961 aacagaatca aagaatatat acagcccgtt aagaaaagtt ccgggaaatt tgaaaagtac
     1021 taccaaacta ccttctctga aaacggaaga agattcctcc ccaaatgaaa cttcagggca
     1081 ggaaccggca aatccgggta agagaagaga agaaatagat cctgaagctc tgaaatcttt
     1141 ttggaattct ttcaaagaga aaagaaaaga actgggaaga gacagggaat taaatgttct
     1201 gaatcaggaa tttgatctca aagatgaaga aattcaactc cagttactta atcctattca
     1261 aggagatatt ttggaggatt tcagggaaga actgcttgac taccttcgct caaaactgaa
     1321 aaatgatttc atatctctaa agacaaggat ggtgaaacag gatgcaaaaa aaatgatcta
     1381 tacatccaaa gaaaagttgg atcatcttat tcagaaaaac cctcaactta aaaatctgaa
     1441 agatagctta ggtctggata acgaatacta agccgtcatc aactttattt cacgtaaggc
     1501 tgtttcggta agaggttttg taatgaactt gataacgtaa ttattgttga cgattttgtc
     1561 aatatcacgc ttgttatctg agctcgaaag aatcactact ttacacttgt tctttattag
     1621 cgcattaaac ttttcaaatt cgtagaggaa tacaaaccca tccacaattg gcatgttaat
     1681 atccaggaag atcaaatctg gcaaatactg agcatcgttc ttattttctt caagaaaatc
     1741 aagagccgct ttgccggaac tttttacctc tacatctttg gcgaaatttg taatttcaat
     1801 aattcgtttg ctgataaaat tatcggtgtc attgtcatct accaataaaa ccttttcaat
     1861 acttaaatct gctttcatgt ttgtttgcat tgctcattta ttcaaagtcc aatttaatga
     1921 agtaataata aggcaaaact aacaattcaa ataagcctta ttcaggtaag gaaatttgtc
     1981 cattattaat ttgagataag atgattatcc ctaagaaagc gtgattattt tttaatcagt
     2041 agcgtctctc caatcttcag ttcacttgaa ttcttgttat tccacttcaa caggtcattt
     2101 aatgacacat catacagttt tgaaattcgg tatagcgtat cccccgcagc tacctcatgc
     2161 tttgtgtaag ttttatccaa ttctttttcc agcttcatct tttcctgcat aatcttcaat
     2221 tcctggtcca tttttaggga attgacatcc tctattgaat tccatactct gagttcttca
     2281 attgaaattc cata
//
LOCUS       DASWHX010000039        16459 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_1140464, whole genome shotgun sequence.
ACCESSION   DASWHX010000039 DASWHX010000000
VERSION     DASWHX010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 16459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16459
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1140464"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            276..566
                     /locus_tag="VGA21_02195"
     CDS             276..566
                     /locus_tag="VGA21_02195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649346.1"
                     /translation="MIYIISGIKKTFSRRLRGFAQKAPEINSAMIYIISGIKKTFSRR
                     LRGFARKTPEINSAMIYIISGIKKTFSRRLRGFAQKTPEINCAMICDISGVL"
     gene            complement(640..2109)
                     /gene="cls"
                     /locus_tag="VGA21_02200"
     CDS             complement(640..2109)
                     /gene="cls"
                     /locus_tag="VGA21_02200"
                     /inference="COORDINATES: protein motif:HMM:TIGR04265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HEX9649347.1"
                     /translation="MIIDWNQILSIASIVIIIAYQLFILLMLLFLLLDNRRPEKTIAW
                     ALVLIMLPVAGLIAYMVMGRNLRKEKLLDRKSLMDDERVIRLRTQQLNHLLKNELEFS
                     DAVQDKKNIITFLLKSSKAILSEFNSINHFTKGSDAFESIFNDLERARHHIHLEYYIF
                     EPDSTGSSVKNILIQKARQGVEVRFIYDSVGSWGLTRKFLNEMKDAGVETREFLPVQF
                     PWFTSRVNYRNHRKIIVIDGTVGYVGGMNVADRYIDGTPKLGAWRDSHLRLEGGSVKA
                     LQLVFCVDWDFVNGSAMANVENYFPPTIIKSRKFVQIAASGPDSETSYMMETFFATMV
                     TARKNIYITTPYFLPNESILTALRTAAFAGVEIKIIIPRKSDSYMVSFASLSYIQSLL
                     KLGIRVFFYRKGFIHAKTMTVDSIISSVGTANMDYRSFDYNLEVNAIIYDEEFAREME
                     DQFNRDLEFSEEIFLDRWRKRKLTDKIRQSAARLFAPIF"
     gene            2371..3441
                     /locus_tag="VGA21_02205"
     CDS             2371..3441
                     /locus_tag="VGA21_02205"
                     /inference="COORDINATES: protein motif:HMM:NF023828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3667 domain-containing protein"
                     /protein_id="HEX9649348.1"
                     /translation="MVREFFVNYFSLDSRLGNSIKPFLFKPGLLTNRFHDGKRLSFMN
                     PVRLYIILSLFYFFVFTKAGQYLVRDKQIDPIIITRTESLDRIQGIDRDLQRKLNANF
                     DSTELAYINPKLDEPTLYNFYTSLNDSQVTIIYPLLKEAGIDSAKIFDPDKMNIVSSG
                     NTSFSVNKTESESFILNRIDFELLNRMIKDNRPDQAIYDSLKLGEITFMEDLVVRQLI
                     RINKADEETVTAYILQNVPLMMFLLIPLFALILKLFYVRRKVLYIRHMVHAIHLHSFA
                     YLIYGLSIIFMFWVVGETASTFLGISAFLWVSVYSYISFRKVYKQGWFKTLVKFLLTG
                     VVYLFLIWVFFIAELLTSALLF"
     gene            complement(3415..5202)
                     /locus_tag="VGA21_02210"
     CDS             complement(3415..5202)
                     /locus_tag="VGA21_02210"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9649349.1"
                     /translation="MRSLILFSLMVLVACSKGFGQENTLAGFQNSYLKARDHYNAARY
                     SEALEAFIPLTISDNPFQEYASFFYAMSAYNSGDRQLSKDMFLQIKKRFPNWEKMEDV
                     NLWLAKLNFENKDYFQGMYYLGVLNSEPARKNGDNMKGHYFRLESDAVILENLLESFP
                     EERSIAGTLAERISKKPLSEIDFNKLDSLVQKYNLDPAKYKLIDGEKVIKKDEYNIGV
                     LLPFMFDELQPQRGRWSNQFILDIYEGMMYGKKELDKLGIRVNLFAFDTRRDSTVTAS
                     LVAGGQLDVMDVLVGPLYTGPSKVIADYSFQKRKNIINPLSANSEITGNNPYSFLYQS
                     SSQSLAKAAADYVHGKFDNKNAAIFYGDSPQDEILANEYRKLIVEDSFTVVIFQKVSR
                     NETRIIFETLMSTYQVPDSLDIDEERETLDVLFLKPDSIGHVFAASDDLLFGSTMIST
                     IENRGDAIQLIGSEEWLQSRSVDYNVVEKLGVWFLSPNYFPTQNEKYQKFREDYLNKN
                     FELPTKNILIGYNLSLFLGMNMFKYGKYFQIGASEHEFEGGYLRTGFEIGPWNDNAWI
                     PIITMRSGEQIWLNKEQMPALSEQKSGGK"
     gene            complement(5203..6732)
                     /gene="guaA"
                     /locus_tag="VGA21_02215"
     CDS             complement(5203..6732)
                     /gene="guaA"
                     /locus_tag="VGA21_02215"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011584328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEX9649350.1"
                     /translation="MAEMILILDFGSQYTQLIARRVRELNVYCEIHPYNHYPAISDNV
                     KGVILSGSPCSVKDKDSPRVNLKEFEDIIPVLGICYGAQLIAEENGGSVAASEIREYG
                     RAKLSQIDHHYELLKELTLDTQVWMSHADTIKNLTPEFDVIAGTSSVFVAAFKHKKKE
                     VWGIQFHPEVTHTTEGKTLLRNFVVHICRCKQNWTPDIFVETTVSQLKNQLGNDKVVL
                     GLSGGVDSSVTAFLIHQAIGKNLYAIFVDTGLLRKNEFEDVIESYSDLGLNVKGIDAK
                     NKFYNALIGLSEPEAKRKAIGKTFIEVFDAEAHKITDVKWLAQGTIYPDVIESVSVKG
                     PSATIKSHHNVGGLPEKMNLKVVEPLRSLFKDEVRNVGRTLEIPDAILERHPFPGPGL
                     GIRILGEITPEKVRIIQEVDDIFIKGLKETGLYNQVWQAGSVLLPIHSVGVMGDERTY
                     ENVVALRAVSSVDGMTADWSQLPFDFLSEISSRIINRVKGVNRVVYDISSKPPSTIEW
                     E"
     gene            6931..8391
                     /locus_tag="VGA21_02220"
     CDS             6931..8391
                     /locus_tag="VGA21_02220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649351.1"
                     /translation="MNYKVMNTKGQKFKIILILIGILVATVAWSQTTEFSSSRDKKSF
                     RWSNNNILQDFNVEYRGDIELTDDDKDVKSISPGGYLEISKTTFGNRREIIIRESGGQ
                     LVREYYEGRSKVPFDPNGKAWLADILPDLVRSTGIAARSRVNRFYKKAGVNGVMDEIE
                     IISSDYVKQIYAKELYQLPLTNAELASTIIDLSRQVSSDYYLAQIMTSHASKFMKSEN
                     TSAAYMQASKNIGSDYYNAQVLTKALEDSKLSASVLKALLESASGMGSDYYMATVLQK
                     AIAEQEMNSSNLSLVVNATENMSSDSYRSTVLKEALKHPSITSDSFASLMRSIGDMSS
                     DYYISTVLKDLLDNHSGDLEQQFLNQIIQTTEDMSSDHYKSLILTQVVTEFDLSDTEF
                     KAIGESVMRMSSDYYVSTVLVKAANKENLSSNSMIAIIKASEQIASDHYKTEVLKKIA
                     PKVKKDDQRAKDAFKQAVKSIRSDHYYGQLMRYLDF"
     gene            8459..9547
                     /locus_tag="VGA21_02225"
     CDS             8459..9547
                     /locus_tag="VGA21_02225"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX9649352.1"
                     /translation="MKFIINFLSMIKRYALGLVVCSVLGLFIFFFLFYTQTGIFPDQK
                     FFIELTASIVTANFIGFILYKINIQLDKIISWRERASLRLLTGIISNYLITSVLGLSI
                     IGALIVYRNEIEFMEIFSQFRESIVKAAIIVLCIILIYSVIHMTLFSYHEYSVVQIEK
                     VKLERKQIALQFEALRNQLSPHYLFNCFNTISSLVYKDTELAETFIRGLAQTYQYVMM
                     NNENQLVKVSGEIEFVKSYYYLMKVRFDNSIDLSIRISEAVSSTLIPPLTFQILVENA
                     IKHNIFSKDAPLKIEINSINEKEILISNNILGQPKKVTSFKVGLENITRRYNFFTNRP
                     IQIDKGSEEFKVILPVIETDFLKKKNVA"
     gene            9544..10596
                     /locus_tag="VGA21_02230"
     CDS             9544..10596
                     /locus_tag="VGA21_02230"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX9649353.1"
                     /translation="MKKLFIHRPLFRIVMPPIYGTLVYFLILLINNSILSIYETFFSQ
                     EVYLSIVLTYIVMEVNHLGIYLSEKFFKSLSLRNKVLIQLGINSTLTLLVVFLTISSY
                     FTYILGFSTYLTEQIYFSVIFLVTSWMYSMINFSHMYLHLQNQELLDEETQLRQSVEA
                     EFQTYQYEMNPMLLFESLESLISLVHKNPVAAEDYIDRLSVAYRYILTNKRNEVVNVL
                     EELKAVDNLVCLQNVRYNNKIEFVYSISGDDEQRKIVPGTFTSILEMIIRRSIINDSQ
                     PIQINCFMETDGYFTLQYKSNDRLVPGYPGKNIVEKLEKTYSFLSDMPVVQVEAFGET
                     YIKIPVLEIIADEVLI"
     gene            10604..11386
                     /locus_tag="VGA21_02235"
     CDS             10604..11386
                     /locus_tag="VGA21_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009579026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEX9649354.1"
                     /translation="MKVVIIEDETPAAEKLQRYVQRYDPGIEVLAQLDSVESSIQWIQ
                     ENQSSVDLFFMDIQLLDGLSLEIFDQVNLEKPVIFTTAFDQYAIEAFKVNSIDYLLKP
                     IIFEDLSNAIKKWERLRKDISPDKSPQIGLEEYRKALAIIQGKKSYKTRFMVKLGEHI
                     RSIPTDKVAVFYAEGRNGFIRTTNGGKFVIDYKLEELETLLDPALFFRVNRTYIVNLH
                     SIKDVIVYSNSRLKVITDPVFEKEIIVSREKVGSFKEWFDGR"
     gene            complement(11703..12086)
                     /locus_tag="VGA21_02240"
     CDS             complement(11703..12086)
                     /locus_tag="VGA21_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013927624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HEX9649355.1"
                     /translation="MIITENEISYKIRGAIFKVFNELGPGLLESAYEAALMYELQKEG
                     LSVKSQVPIPLVYDNIKLEIGFRIDLLVENKVVVEIKSVENLAEVHHKQVITYLKLSG
                     KKLGLLVNFNCAQIEKAIYRKVNGL"
     gene            complement(12342..12689)
                     /locus_tag="VGA21_02245"
     CDS             complement(12342..12689)
                     /locus_tag="VGA21_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018478332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor"
                     /protein_id="HEX9649356.1"
                     /translation="MAKKESWKGREGIVYSTLEDFAYQNRKKVESFQAPLKQDLRVWL
                     DKKSRAGKKVTLVKGFKGNPDHLKSIATLLKSKCGVGGTAKDGEILIQGDFRDKVVEI
                     LQQEGYKAKIAGG"
     gene            complement(12694..13539)
                     /locus_tag="VGA21_02250"
     CDS             complement(12694..13539)
                     /locus_tag="VGA21_02250"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX9649357.1"
                     /translation="MKNIIVPTDFSKEAGYAVDAAAMLARKLGSTVQLLHIVEAPSAQ
                     SFNVTGEIRGPSTIDDVFLIKLIERVKSQLNKIVSAPLFKDVRVSYQVDVGAVVPSIK
                     KAVTKAKADLVIMGTQGVSGWNEVLIGSNTEKVVRMISCPVLTLKGALKPANLKSIAF
                     ATDMGPKQAKIVAQVKQFQTALNAKLHIVTINTPANFRSDRLTNPKLESFAKSNNLSN
                     YTLNIHNDAVEGDGIMSFALQKQVGMIAMATHGRTGLAHILSGSIAEEIVNHSKIPVM
                     TFNIK"
     gene            complement(13596..14507)
                     /locus_tag="VGA21_02255"
     CDS             complement(13596..14507)
                     /locus_tag="VGA21_02255"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009193519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046428 - 1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HEX9649358.1"
                     /translation="MSSTALKSWISALRLRTLPLALASIGMGSFLAAYAGRFNLPIFS
                     FSALTTIFLQILSNLANDYGDSAHGADHSLRKGPNRMVQSGLISKSAMRFAILVLIVL
                     SLVSGIYLLYLAFGWNGGAFLVFLLIGITAILAAIAYTAGSKPYGYSGLGDLSVLIFF
                     GLVGVLGTYYLYTGELYPSLILPALSCGFFSISVLNLNNIRDIESDKMAGKKSIPVRL
                     GRERAVIYHEVLLIAGTACALAFVIIEVKEWTAFIFVITTPLFVQNFLAVKNIQDPTK
                     LDPYLRQMAISTLIFVLVFGIGLLFSI"
     gene            complement(14507..15076)
                     /locus_tag="VGA21_02260"
     CDS             complement(14507..15076)
                     /locus_tag="VGA21_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015266269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEX9649359.1"
                     /translation="MKQLFVTILILTSCGFNSISSRPDDAIIPLDVPTFYEFKMKSIT
                     GEEISFDQYKGKKVLVVNVASKCGYTPQYEDLQKLHEQFGDKVTILGFPANNFMSQEP
                     GTNEEIANFCKTNYGVTFQMFEKISVKGNDMAPLYQWLSSKELNGWNDKSPKWNFCKY
                     LINENGELVEYYTSGVNPMSDEILQAILK"
     gene            complement(15079..>16459)
                     /gene="argS"
                     /locus_tag="VGA21_02265"
     CDS             complement(15079..>16459)
                     /gene="argS"
                     /locus_tag="VGA21_02265"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010610690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEX9649360.1"
                     /translation="NFLGYSVAKILEANGNTVHKVQIINDRGIHICKSMLAWIKFGKG
                     ETPESAGMKGDKFVGKYYVKFDQIYREEVRQLMAEGISKEDAEKEASIMKEAQELLKK
                     WEDKDTEAYSLWEKMNQWVYDGFEKTYKTMGVEFDRLYYESETYLEGKNEVLKGVESG
                     NFTRKEDGSVWVDLTDEGLDEKLLLRKDGTSVYITQDIGTAMMRFRDYPGLSRIIYTV
                     GNEQDYHFKVLFLILKKLGVEWYKGLYHLSYGMVDLPTGRMKSREGTVVDADDLMEEM
                     IITAEKHTRELGKIEGFEEEQAKDLYRILGLGALKYFLLRIDPRKRMQFDPEESIEFQ
                     GNTGPFIQYTHARIASILRKAAELNFDPRGSFSMTEINRYERDVLFLINDFENRIDEA
                     AKELSPSLIAQYVYEIAKEYNKFYSEISIFNEKDPEKIKARVQLSYITSRTIKAAMLL
                     LGIEVPDRM"
BASE COUNT         4923 a         3394 c         2994 g         5148 t
ORIGIN      
        1 aactcccgaa ataaattcag aggtgatctg cgatatcagc ggggttaaga aaaattttct
       61 cccgcagatt acgcggattt gcacagaaaa ctccggaaat aaattcagag gtgatctgcg
      121 atatcagcgg gattaaaaaa aattactccc gcagattatg cggatttgcg tagaagactc
      181 cggaaataaa ttctgcggtg atctgcgata tcagcgggat taaaaaaact ttctcccgcg
      241 gatttgcgcg gaaaactccg gaaataaatt ctgcgatgat ctacattatc agcggaatta
      301 agaaaacttt ctcccgcaga ttacgcggat ttgcgcagaa agctccggaa ataaattctg
      361 cgatgatcta cattatcagc ggaattaaaa aaactttctc ccgaagatta cgcggatttg
      421 cgcggaaaac tccggaaata aattctgcga tgatctacat tatcagcgga attaaaaaaa
      481 ctttctcccg cagattacgc ggatttgcgc agaaaactcc ggaaataaat tgtgcgatga
      541 tctgcgatat cagcggggtt ctataaaatt tgctcccgca gattacctga atttgcagga
      601 tttagcttta ctagaacaaa gccgcaaaaa aagattcggc tagaaaatag gcgcgaacaa
      661 ccgtgcggca gactgtctga tcttatcagt tagctttctc tttctccatc tgtcaaggaa
      721 tatttcctcg ctaaactcca ggtctctgtt aaactgatct tccatttccc tggcaaattc
      781 ttcatcataa ataatcgcgt tcacctccaa attataatcg aagcttcgat aatccatgtt
      841 agcagttccc acagaactga ttatgctatc cactgtcatt gttttggcat gtataaatcc
      901 ttttcgataa aaaaataccc gaattcccag tttcagcagt gattggatat aagataaaga
      961 ggcgaaactc accatatagg aatccgattt tctgggaata attattttta tttccacacc
     1021 ggcaaatgct gccgtgcgaa gtgcagtcaa gatgctttca ttaggaagaa aatacggtgt
     1081 ggtaatgtaa atatttttgc gagcagtcac cattgttgca aaaaatgttt ccatcatgta
     1141 actcgtctca gagtcaggac cactggccgc aatttgcaca aactttcggc tcttaataat
     1201 agtgggaggg aagtaatttt ccacattagc catggcactg ccattaacaa agtcccagtc
     1261 gacgcaaaaa accaattgca aagcctttac cgagccaccc tcaagtcgca aatgagaatc
     1321 ccgccaggca cccagctttg gagttccatc tatgtatcga tcggcgacat tcattccacc
     1381 cacatatcca acagtgccgt caattacaat tatttttcta tggtttctgt aatttacgcg
     1441 gctggtaaac caggggaatt gcacgggaag aaattcccgg gtttcaactc ctgcatcttt
     1501 catttcatta agaaatttcc tggttaggcc ccagcttcct acagaatcgt aaatgaatcg
     1561 cacttctacg ccttgtctgg ctttctgaat aagaatattt ttaaccgagc tccccgttga
     1621 atcgggttca aaaatataat attccaaatg aatatgatgc cgggctcttt ccaggtcatt
     1681 aaaaatagac tcgaaagcat ccgatccttt ggtgaagtga tttatggaat taaattcaga
     1741 gagtattgcc ttgctgcttt tcaaaagaaa tgtgatgata tttttcttgt cttgaacggc
     1801 atcgctaaac tctaattcat ttttcaatag gtgattcaat tgttgcgtgc gcaaccggat
     1861 cactcgctca tcatccatca ggctttttct atcaagtaat ttttccttac gcaaatttct
     1921 tcccataacc atgtatgcta ttaatccggc aaccggaagc ataatcagca ccagtgccca
     1981 tgcgatggtt ttctcgggcc tgcgattatc cagtagcagg aaaagtaaca taagcaaaat
     2041 aaacagttga taggcaatga taatgactat acttgctatt gataatatct gattccagtc
     2101 tataatcata cgggtgctta actcctaatt tacaaaggaa ggagtattgg attggcaact
     2161 atttagcatt taatgataaa aattggaaaa ttgaaagtag aaaatattta ttcgtttgca
     2221 aattcttaat ctatttgctt tatttcgccg ttgaaaaatc gaaggaaatc tgatgtatgt
     2281 ctgaattgcg gttcagacct cagcctggaa tttaattttt gcccaaagtg cgggcaagaa
     2341 aacaataaca gccaggtttc ctttgcgcaa ttggtcaggg aattttttgt taattatttt
     2401 tcattagaca gcaggctggg gaattccata aaaccttttc ttttcaaacc gggtcttttg
     2461 acaaacagat tccatgatgg aaagaggtta tcatttatga acccggtacg gctatacatt
     2521 attcttagtt tattctactt ttttgttttt actaaagcag gtcaatacct ggttcgggat
     2581 aaacaaattg acccaataat aatcacccga acagaatctc ttgataggat ccagggtatt
     2641 gaccgcgatt tgcagcgtaa gttgaatgcg aattttgaca gcacagaatt agcttacatt
     2701 aatcccaaac ttgatgaacc aactctttat aacttttata caagcctgaa cgatagtcag
     2761 gtcacaataa tctatcctct cctcaaagaa gccggcatag acagtgcaaa gatttttgat
     2821 cctgataaaa tgaacatcgt aagttccgga aatacgtcgt tctctgtaaa taaaacagaa
     2881 agtgaatctt tcatcctgaa tagaattgat tttgaactgt taaacaggat gatcaaagat
     2941 aaccggccag accaagctat ttatgattcg ttgaagctag gtgaaattac ttttatggaa
     3001 gacctggtgg taaggcaact gatcaggatt aacaaggccg atgaagagac ggttactgcc
     3061 tacatcctgc aaaatgttcc cttaatgatg tttctgctga tacctctgtt tgctttgatt
     3121 ctcaaattat tttatgtgag gaggaaagta ctatatatca gacatatggt acacgccatc
     3181 catcttcatt cctttgcgta cttaatttat ggcttatcca ttatttttat gttttgggtg
     3241 gttggtgaga cggccagtac atttctggga attagcgctt tcctgtgggt aagtgtctat
     3301 tcatatatct ccttccgaaa agtttataaa caaggatggt ttaagacgct ggtgaagttc
     3361 ttacttaccg gcgtggtata tttatttcta atctgggtat tctttatcgc agaattactt
     3421 acctccgctc ttttgttctg aaagggcggg catttgctct ttattcagcc agatctgttc
     3481 tccgcttcgc attgtgatga ttggaatcca ggcattgtca ttccagggac ctatctcaaa
     3541 accggtcctc aggtagcctc cttcaaattc atgttccgaa gctccaattt gaaaatactt
     3601 cccatacttg aacatattca ttccaagaaa caaacttaaa ttgtaaccaa tcaaaatgtt
     3661 cttggtaggt aattcgaaat ttttatttaa gtaatcttcc ctgaactttt gatatttctc
     3721 attttgagta ggaaaataat tgggagataa aaaccaaacg ccaagtttct ccacaacatt
     3781 ataatccacc gaccttgatt gaagccattc ttcggatcct attaactgaa tcgcgtcgcc
     3841 gcggttttca atggttgaga tcatggttga accaaaaagc aaatcatcag atgctgcaaa
     3901 aacgtgtccg atgctgtcgg gctttaaaaa caaaacatca agggtttccc tttcttcatc
     3961 aatatccaat gaatcaggta cctgatatgt tgacataagg gtttcgaata ttatgcgggt
     4021 ttcattccta ctcactttct gaaatatcac caccgtaaaa ctatcctcaa caatcagttt
     4081 tctatactca ttagccagga tttcatcttg aggcgaatct ccgtagaata ttgcagcgtt
     4141 cttgttatca aattttccgt gaacataatc cgctgccgct ttagccaaag attgtgaaga
     4201 agactgatat aaaaatgaat agggattatt tccggttatt tcagaattag ctgatagggg
     4261 attaatgata ttttttcttt tctgaaaaga ataatcggcg atcactttgc ttggacctgt
     4321 atataatggt cctaccaaca catccatgac atcaagttgt ccgccagcga caaggcttgc
     4381 ggtaactgta ctgtctcttc tggtatcaaa agcaaacaaa tttacccgga tgcccagttt
     4441 atccaattcc tttttcccat acatcatacc ttcataaatg tccaggataa actgattact
     4501 ccacctcccc cgctgtggct gcaattcatc aaacatgaat ggtaaaagta cgcctatgtt
     4561 atattcgtct tttttaataa ccttttcacc atcaattagt ttatattttg ccggatccaa
     4621 attgtatttc tgaaccaatg aatccagttt attaaaatca atttcactca aaggtttttt
     4681 tgaaattctt tctgccaggg taccggcgat tgaccgctct tcggggaagc tttccagcag
     4741 attttccagg ataaccgcat cactttcgag cctgaaataa tgacccttca tgttatcacc
     4801 attctttcgg gctggttctg aattcagcac acccaggtaa tacatgccct gaaaataatc
     4861 tttgttttca aagttaagtt tagctaacca gagatttaca tcctccattt tttcccaatt
     4921 aggaaatcgt tttttaatct gaagaaacat atcttttgag agttgcctgt ctccagaatt
     4981 gtaggctgac atagcataga aaaaagaagc atattcctga aagggattat cagaaattgt
     5041 caaaggtata aaagcctcaa gtgcttcaga ataccgcgcg gcgttgtaat gatcccgggc
     5101 tttcaaataa gaattctgaa aaccggctaa agtgttttcc tgtccaaaac cttttgaaca
     5161 ggcaaccaga accattaagc taaaaagaat taaagatcgc atttattccc attcaatggt
     5221 tgaaggaggt tttgagctga tatcataaac aacacggttt acacccttta ctctgtttat
     5281 tatcctgctt gagatttcac tcagaaaatc aaagggcaac tggctccaat ctgccgtcat
     5341 tccatctaca ctggatactg cccgaagtgc gacaacattc tcgtatgttc tttcatcacc
     5401 cataaccccc acagaatgaa ttggcagaag aactgaaccg gcttgccaaa cctggttgta
     5461 taaaccggtt tccttcagcc cttttataaa aatatcatcc acctcctgaa taatcctaac
     5521 tttttcagga gtaatttctc ctaagatgcg aattccaagg cccggtcccg gaaatggatg
     5581 tctttccagg atggcatccg gaatttccag cgtccttcct acattgcgaa cttcatcttt
     5641 aaaaagtgat cgcaatggtt caacaacttt caggttcatt ttttcaggta atccacccac
     5701 attatgatga cttttaatag tcgctgatgg ccctttcaca gaaacggatt caatcacatc
     5761 agggtatata gtgccttgtg caagccattt gacatccgtt atcttgtgag cctccgcatc
     5821 gaaaacctca ataaaggttt taccaattgc tttccttttt gcttccggtt cactcaatcc
     5881 aattaatgca ttataaaatt tattttttgc atcaattccc ttaacgttca aacccagatc
     5941 gctgtacgat tcgataacat cttcaaattc attcttcctg agaagaccag tatccacaaa
     6001 aattgcataa agatttttgc caatcgcctg atgaatcaga aatgcagtta cagaactgtc
     6061 tacaccacct gaaagaccca gcactacttt atcgtttcca agttgatttt ttagttgtga
     6121 aaccgtggtt tcaacaaaaa tatcgggtgt ccagttttgc ttgcaccggc agatatgaac
     6181 tacaaaattt ctaagcaaag tcttaccttc tgtagtatgt gtaacctcag gatgaaactg
     6241 aattccccaa acttcttttt tcttatgttt aaaagctgcc acaaatactg aagaggtacc
     6301 ggcgataaca tcaaattccg gagtaagatt tttaatcgta tccgcgtggg acatccaaac
     6361 ctgggtatcc agtgttagtt ctttaagtag ttcgtaatga tgatcaattt gtgaaagttt
     6421 agctctgcca tactcacgaa tttcagatgc tgccacactt cctccattct cttccgcaat
     6481 taattgtgcg ccataacata tcccaagcac cggaattata tcttcaaatt cctttagatt
     6541 tactctgggc gagtctttat ctttaaccga gcatgggctt cccgataata taactccttt
     6601 tacattatca cttatagcag gataatgatt gtacgggtga atctcacaat acacattgag
     6661 ttctctaacc cgccttgcta tcagctgagt gtattgtgac ccaaaatcaa gaattaatat
     6721 catttccgcc atctgacaaa aatactcaga ctcatcacag ttccttatat aaataagtca
     6781 ttttttgtca aaactttctc aaatattggt acttgctatt aacagttcag tccatcttta
     6841 ttacagtttg attcatctgt ttttaaacag tggtaacaat aacccaatat tgtgtcgtaa
     6901 tacttaatga aaagaaacaa ctctcaaaga atgaattata aagttatgaa tactaaaggt
     6961 caaaaattca aaataatttt aatccttatc ggaatcctgg ttgccacagt tgcctggagt
     7021 caaactacag aattctcaag ttcgagggat aagaaatctt tccgatggag caataataat
     7081 attcttcagg atttcaacgt ggaatatcgc ggtgacatcg aacttactga cgatgacaaa
     7141 gacgtaaaga gtatttctcc gggcggatac ctggaaatca gcaaaacaac ttttggaaac
     7201 cggagagaaa tcattatcag agaatcgggc ggtcagttgg ttcgtgagta ttacgaaggc
     7261 cgctcaaaag ttccgtttga cccaaatgga aaagcctggt tggccgatat tcttcctgac
     7321 ctggttcgtt ccaccggtat cgccgcacga agcagggtaa atcgttttta taagaaggcc
     7381 ggtgttaatg gagtgatgga tgaaatagaa attattagca gcgattatgt aaagcaaatt
     7441 tatgctaagg agttatacca gcttccttta accaatgcgg aactggcaag cacaattatt
     7501 gatttatcca gacaggttag ttctgattat tacctggcgc aaataatgac aagccatgcc
     7561 agtaagttta tgaagtcaga aaatacttcg gccgcttaca tgcaagccag caaaaacatt
     7621 ggaagtgatt actacaatgc ccaggtatta accaaagctt tggaagattc aaaactatca
     7681 gcttctgttc tgaaagcgct gcttgagtcc gcctccggta tgggttctga ttattacatg
     7741 gctacagttc tgcagaaagc tattgctgag caggaaatga attcctccaa cctgagtctc
     7801 gtggttaatg ccaccgagaa catgtcatca gactcctacc ggtctacggt tttaaaagag
     7861 gcgctcaaac atccgagtat aacctccgat agttttgcaa gtttgatgag atcaattggt
     7921 gatatgtctt ctgattatta tatctcaacc gttttaaaag atcttctgga taaccatagc
     7981 ggtgatttgg agcaacagtt tttaaatcaa ataattcaaa ccacggaaga tatgtcttcc
     8041 gatcattaca aatctttaat tcttactcag gtagtcactg aatttgattt aagtgatact
     8101 gaatttaagg caattggaga gtctgtaatg aggatgtcgt ctgactatta tgtttctacc
     8161 gttttggtta aagccgcaaa caaagaaaat ttgtcatcca attctatgat tgcaataatc
     8221 aaagcttccg aacagatagc atctgatcat tataagactg aggtgttaaa gaagattgct
     8281 cccaaggtta aaaaggacga ccagcgggcg aaggatgctt ttaaacaagc tgttaaaagc
     8341 attcgttctg atcattatta tggtcagcta atgcgttacc tggatttttg aacaagtcaa
     8401 tgctttgaga ttactcccta attggcgaat ttgccgtcag ggataatctg cagagcaaat
     8461 gaaattcatt ataaattttc tttccatgat taagagatat gcactcggac ttgtggtctg
     8521 ttcggtctta gggttattca ttttcttttt tctgttctat acacaaaccg gaatcttccc
     8581 ggatcaaaag ttcttcatcg aacttactgc ttccattgtt actgccaatt ttatcggttt
     8641 cattctttat aaaatcaaca ttcaacttga taaaataatt agttggagag aaagggcatc
     8701 gttacgactt cttaccggaa ttatttcaaa ctatcttata acttctgttc tgggcctctc
     8761 cattattggt gcattaatag tttacagaaa tgaaattgaa ttcatggaga tattttctca
     8821 attcagggaa agtattgtaa aagcggccat catcgttctt tgtattatcc ttatatactc
     8881 tgtgattcac atgactttgt tttcttacca tgaatattca gtggtacaga ttgaaaaagt
     8941 aaagctggag cgaaaacaaa ttgcacttca gtttgaagct ttgcgcaacc agttaagccc
     9001 gcactacctt ttcaactgtt ttaatacaat ttcttcgttg gtttacaagg atacagagtt
     9061 agcagaaact tttatccgcg ggcttgctca aacctaccag tatgtaatga tgaataatga
     9121 aaatcaactg gttaaggtta gcggggaaat tgaatttgtg aagagctatt attatttgat
     9181 gaaggtaaga tttgataata gtattgattt gagtatccgg atctccgaag ctgtttcatc
     9241 aaccttaatt cctccactta cttttcagat attagtggaa aatgcaatta agcataatat
     9301 cttttcaaaa gacgctccct taaaaatcga aattaattcc attaacgaaa aagaaattct
     9361 tatttctaac aatattctgg gtcagccgaa gaaagtaact tcatttaaag taggactcga
     9421 aaatattact cgccggtata acttttttac aaaccgtcca atacaaatag acaagggaag
     9481 tgaggagttt aaagtgattc ttccagtgat tgaaactgat tttttaaaga agaagaacgt
     9541 agcgtgaaaa agttatttat tcaccggcca cttttcagaa tagtaatgcc acccatttat
     9601 ggaacgctgg tctattttct tatattatta atcaataatt ccattctttc tatttatgaa
     9661 acattcttta gccaggaagt ttatttaagc attgtgctta cttacatagt tatggaagta
     9721 aaccatcttg ggatttattt gtctgaaaaa tttttcaaga gtcttagttt aaggaacaag
     9781 gttttgattc agctgggaat taactccaca ttaactttgc tcgtggtatt cctgaccatc
     9841 agtagctact ttacctacat acttggtttc agcacctatc tcacggaaca aatctacttt
     9901 tcggttattt ttctggtaac ctcctggatg tattctatga tcaatttttc ccacatgtat
     9961 ttgcatttac agaaccagga gttgcttgat gaagaaacac aattaaggca gtcggttgaa
    10021 gctgagtttc aaacctatca atacgagatg aatccaatgc ttttatttga aagcctggag
    10081 tcattaattt ccctggtaca taaaaatccg gttgctgctg aagattatat tgacagatta
    10141 tcagttgctt atcggtatat tttaacgaac aagcggaatg aagttgtaaa tgtcttggag
    10201 gaattaaaag cagttgataa cctggtctgt ttacagaatg ttcgatacaa taataagatt
    10261 gaatttgtat attcgatttc tggcgatgac gaacaaagaa aaattgtccc tggtactttt
    10321 acttcaattc tggaaatgat tattcgccga agcattatca atgatagtca accaattcaa
    10381 ataaattgct tcatggaaac cgatggatac tttactcttc aatataaatc aaatgaccgg
    10441 ttagttcccg gttacccagg caagaacatt gtggaaaaac tggagaagac ctattctttt
    10501 ttgagtgata tgcctgttgt tcaggtagaa gcatttggtg agacctatat aaagatccct
    10561 gtcctggaaa taattgcaga tgaagtattg atataagaga ttaatgaagg ttgtaattat
    10621 agaagatgaa acacctgcag ctgaaaagct gcagcgctat gtgcaaagat acgatcccgg
    10681 aattgaagtt ctggcccaat tggactcggt ggaaagttca atacaatgga ttcaggagaa
    10741 tcaatcttcc gtagatctgt tttttatgga catacaactc ctggacggcc taagccttga
    10801 aatctttgat caagtaaatt tggagaaacc tgttattttc accaccgcgt ttgatcagta
    10861 cgccattgag gcttttaagg tgaatagtat tgactacctg ctaaagccga taatatttga
    10921 agatctctcc aacgccatta agaaatggga aaggctccgc aaagacatct ctccagacaa
    10981 gagtccacaa ataggcttgg aggagtatcg aaaggctctt gcaattattc aggggaagaa
    11041 gagttacaag acccgtttta tggtgaaatt gggggaacac attcgttcga ttcccactga
    11101 caaagtagct gtattttatg ctgaaggaag aaatggattt atcagaacaa ccaatggcgg
    11161 aaagtttgtt attgattaca agctcgagga acttgaaact ctccttgacc ctgcactttt
    11221 ttttagagtt aaccgaacct atattgtaaa tctgcattca attaaagatg ttatagttta
    11281 ctctaattca agattgaaag taataactga tcccgtcttt gaaaaggaga ttattgttag
    11341 ccgtgaaaaa gtaggttcat tcaaagaatg gtttgatggc agataaattt tctctcgcag
    11401 atttcgcgca gaaaactctg gaagtcaaat tccgcggtga ttctgcgaga tcagcgggag
    11461 aaaaaaacct ttcccgccga ttgagcggat ttgtgcggaa agctctggaa ctattttctc
    11521 cgcggatcag cgagattcta taaaactctt ttctcccgcg gatttacgca gaaaaaactc
    11581 tttcctcccg cggattacca ggattttagc ggaaaacttt agtgaatcaa ttctgcggtg
    11641 atatgcgaga tcagcgggat taaaaaaaac tcccgcagat tgcgcggatt tgcgcagaaa
    11701 atttataatc catttacttt cctgtaaatt gctttttcta tttgtgcaca attaaaattt
    11761 acaagcaatc caagtttctt gcctgacaat tttagatatg tgattacctg tttatgatga
    11821 acttctgcaa ggttttctac ggatttaatt tccaccacaa ctttattttc aaccaataaa
    11881 tcaatccgaa atcctatttc caattttatg ttatcataaa ccagaggtat aggaacttga
    11941 gattttaccg acagtccctc cttttgtagc tcatacatca gagctgcctc ataagctgat
    12001 tcaagcaagc ccggaccaag ttcattgaat actttgaaaa ttgctcctct tattttatag
    12061 gaaatttcgt tctctgtaat tatcatttag tagaatttaa atacccatct tttatttccc
    12121 gcagattgcg cggatttgtg cagaaagctc ccgtaccatt ttttctgcgg tgatccgcga
    12181 tatcagcggg agaaaaaaac ctttcccgca gattgcgtgg atttgcgcgg aaacctatcc
    12241 aaacattttt ctctatggtg attctgcgat atcagcggga ttaaaaaaaa tctccagcag
    12301 attacgcgga tttgcgcaga aaaatcctca aaccaaattc atcacccacc ggctatcttt
    12361 gctttatacc cttcctgctg aagaatctca actactttat cccggaaatc tccttgaatc
    12421 aatatctctc catctttggc agtgccgcca accccacact ttgacttaag caaagtagca
    12481 atgcttttca gatgatcggg attcccctta aatcctttta ctaaagtaac tttttttcct
    12541 gcacggcttt ttttgtcaag ccaaacacga agatcctgtt tcaacggagc ctgaaatgat
    12601 tctacttttt tgcgattttg ataggcaaaa tcttcgagtg ttgaataaac tatcccttcc
    12661 ctacccttcc agctttcctt tttggccatt aaactactta atgttgaaag tcataaccgg
    12721 aatttttgaa tgattcacaa tttcttcagc aatactcccg cttaagatgt gagccagacc
    12781 tgttctgccg tgagtagcca tagcaatcat gcccacttgc ttttgaagtg cgaaagacat
    12841 aataccatcg ccttcaacgg catcattatg aatattgagg gtataattac tgaggttatt
    12901 gcttttcgcg aatgactcta gtttcgggtt tgttaatctg tctgatcgga aattcgcagg
    12961 agtatttatt gtaacaatgt gaagctttgc atttaaggct gtttgaaatt gtttgacttg
    13021 tgcaacaatt ttagcttgct ttggtcccat gtcggtggca aatgctatag acttaaggtt
    13081 agcgggtttc agcgctcctt ttaaagtcag cacggggcag gaaatcattc gtacaacttt
    13141 ttccgtattt gaaccaatta gaacttcatt ccagccgctt actccctgag ttcccattat
    13201 aaccagatct gcctttgctt tggtaaccgc tttcttaatg gacggtacta cagctccaac
    13261 atcgacttgg taagaaactc taacgtcttt aaaaagtggc gcggaaacaa ttttgttgag
    13321 ctggcttttt actctttcaa taagtttgat taagaaaaca tcatctatgg tggacggtcc
    13381 cctgatttct ccagtgacat taaaagattg cgctgaagga gcttcaacaa tgtggagtaa
    13441 ttgcacagtt gatcccaatt tcctggcaag cattgcagct gcatctacgg cataaccggc
    13501 ctcttttgaa aaatcggtag gaactataat attcttcata atttggttgt attaataaga
    13561 aaataaagat agccaatcta ggttgaaatc ctaaatcaga ttgaaaatag taatcctatg
    13621 ccaaacacta gcacgaaaat taaagtgctg attgccattt gccttaggta gggatcaagc
    13681 tttgtgggat cttgaatatt ttttactgcc aggaaatttt gcacaaataa aggagtggtt
    13741 atcacgaata taaatgctgt ccattcttta acttcaataa taacaaatgc cagcgcgcaa
    13801 gcggttccgg cgataagtaa aacctcatga taaatcactg ccctttcacg tcccagtctt
    13861 acgggaatgg atttcttacc ggccatttta tcagattcga tatcccttat attgtttaag
    13921 tttaacacgg aaattgagaa aaatccgcag ctaagagccg gcaaaataag gcttgggtat
    13981 aattccccgg tatacaaata ataggttcct aacaccccta ccaatccgaa aaagattaag
    14041 acagataagt cacctaatcc tgagtacccg tagggtttac ttcctgcggt gtaagcaatg
    14101 gcagccaaaa tagcggttat ccctatcagt agaaatacta aaaacgcgcc accgttccat
    14161 ccaaaggcca ggtaaagtaa atagatacca ctaaccaagc tcaaaacaat caggaccaaa
    14221 attgcgaatc gcatagcact tttacttatc aagcctgatt gaaccattct atttggacct
    14281 tttcttaaac tatggtcagc accatgggcc gagtctccgt aatcgttggc cagatttgat
    14341 aaaatctgca ggaaaattgt tgtaagcgcg ctgaaactaa aaataggtaa attgaatctt
    14401 cccgcatacg ccgccaggaa acttcccata ccaattgagg caagtgccag gggaagggtt
    14461 cttagccgta atgcggaaat ccatgatttc agagctgttg aagacattat ttcaatattg
    14521 cctgcagaat ttcatctgac ataggattca caccgctggt ataatattct acaagttccc
    14581 cattttcatt tatcaggtat ttgcagaaat tccatttggg cgatttatca ttccagccat
    14641 ttaattcttt cgaagataac cattgataga gcggagccat atcatttcct ttcacagaaa
    14701 tcttttcaaa catttgaaat gtaactccat aattagtctt gcagaaattg gcaatctcct
    14761 cattcgtgcc cggctcctgg ctcataaaat tattagcggg gaatccaagt atggtgacct
    14821 tgtctccgaa ttgctcatgc aatttttgca agtcttcata ttgaggagta tacccgcatt
    14881 tggaggctac attcacaacc aacacctttt tacctttgta ctgatcaaag gaaatctctt
    14941 ccccggttat tgatttcatt ttaaattcat aaaaagtggg tacatccaat ggaataatgg
    15001 catcatcagg tctgctggaa atactgttga atccgcagct ggttagaatc agaattgtta
    15061 caaataattg tttcatagtt acattcggtc tggtacttct attcctaata acaacatcgc
    15121 tgccttaatt gttcgtgaag taatataaga caactgaacc ctggctttta tcttttcagg
    15181 atccttttca ttaaagatcg atatttctga ataaaattta ttgtattctt tggcaatttc
    15241 atagacatac tgagcaatta atgacggaga cagctctttg gcagcttcat caattctgtt
    15301 ctcaaagtca tttatcagga acaggacatc tctctcatat cgatttatct ctgtcattga
    15361 aaaactacct cgtggatcaa agtttaattc tgccgctttc cttaatatgg aagcaattcg
    15421 ggcatgcgta tattgaataa aagggccggt gtttccctgg aactcaatgg attcttcagg
    15481 atcaaattgc attcgcttgc gcggatctat ccgtaaaagg aaatatttca gagctcccaa
    15541 acccaaaatt ctgtataaat cttttgcctg ttcttcttcg aaaccttcaa tctttcccaa
    15601 ttcccgggta tgcttttcag cggttatgat catctcttcc ataagatcat cggcatcaac
    15661 tacggttcct tcccttgact tcattctgcc ggttggtaga tctaccatac catacgaaag
    15721 atgatagaga cctttatacc attctacacc caattttttt aatatcagaa aaagaacttt
    15781 gaagtgatag tcctgttcat tgccaaccgt gtatattatc ctggataatc ctgggtagtc
    15841 cctgaatcgc atcatggccg tgccaatatc ctgggttata taaactgaag tgccatcttt
    15901 acgaagcagc aatttttcat caagtccctc atcagtcagg tcaacccaaa ccgagccgtc
    15961 ttctttcctg gtaaaattcc ccgactcaac acctttcaac acttcattct ttccttccaa
    16021 ataagtctcc gactcataat aaagtcgatc aaactcgact cccatggtct tgtaggtttt
    16081 ttcaaaccca tcataaaccc attgattcat tttctcccaa agagaatatg cttcggtgtc
    16141 cttatcttcc cattttttca acaactcctg agcttccttc attattgaag cttccttctc
    16201 tgcatcttct tttgagattc cctcggccat cagctggcga acttcctctc tatatatctg
    16261 gtcgaacttc acatagtatt ttcctacaaa tttgtctccc ttcatacctg ccgattcagg
    16321 tgtttcacct ttgccaaatt ttatccacgc aagcatagat ttacaaatat gaattcccct
    16381 gtcatttata atctgaactt tatgaactgt attgccattt gcttcaagaa ttttcgctac
    16441 agagtatcca aggaaattg
//
LOCUS       DASWHX010000040         3001 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_769872, whole genome shotgun sequence.
ACCESSION   DASWHX010000040 DASWHX010000000
VERSION     DASWHX010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 3001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3001
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_769872"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..812
                     /locus_tag="VGA21_02270"
     CDS             <1..812
                     /locus_tag="VGA21_02270"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEX9649361.1"
                     /translation="KFDNHKNMIANKELFKAIDRENLLLIRTEIQNGAEPNEKNGYGD
                     TAMHFSITKGNLEILKLLLELGGDINAKNQEGVSLLLQSIGEEKFELSHFLIENKADV
                     NLQGKESESPIHIASRLGNNGLVKKLIQKGAVVNVTDNRLWTPLHYVGYLDNPNLEVV
                     ETLIENGADLNAKDNGGRTCLHYVAMKEGKPKDIKLAGLITNQQNIYIQDNDGNTALH
                     FAAEDSSLKMVECLISKGADLSLRNSRGETPAGLAELANRKAIRKVLNSGK"
     gene            1045..1893
                     /locus_tag="VGA21_02275"
     CDS             1045..1893
                     /locus_tag="VGA21_02275"
                     /inference="COORDINATES: protein motif:HMM:NF020795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1963 domain-containing protein"
                     /protein_id="HEX9649362.1"
                     /translation="MNKVNQAREIIRKYPFQEKQLSDYAKDKIGLYFRKESRGYYYNG
                     KITKLFKVLTPEILAESFHFIINLADTSPTLKMGYSKLFGSPHLPGDWKMPGDWKGMS
                     FWFQINLEEVKPFDKEDLLPEKGIIYVFQHSSEGKCKIYFYTDTLDKLITRADIPVLN
                     YEKEQTLSFEPSFIFYIGGDAYDYSEIYNFIPKALRDDLEKLLECKMDDTDSCTRLFG
                     RPVYWQGEDEEFDEEFDEDQDFESNELALFFQDELDDAAINIWVPKSDLRNGVFENAF
                     ETFSTT"
     gene            complement(1890..2117)
                     /locus_tag="VGA21_02280"
     CDS             complement(1890..2117)
                     /locus_tag="VGA21_02280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649363.1"
                     /translation="MAFAGFSQHGKVSPDCKWMFSGSSLSSNMVLAFCSGGRSCDSWN
                     DIRHESDFRPDLSSLIKDAFVNTRFSYQFFY"
     gene            2228..>3001
                     /locus_tag="VGA21_02285"
     CDS             2228..>3001
                     /locus_tag="VGA21_02285"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEX9649364.1"
                     /translation="MKRNLVILFVFSVVVFQWSPLHSQTTNTSIENKGIVTVKGLVTD
                     EDSQTGVPGVTVMLKGTNTGTVTDRDGKYVINASESGGVLIFSFVGYATQEIELGNRS
                     VIDVVLKPDTYLLEEFVIVGYGEQRKSDITGSISSIEVENLDRLPVASIDDAMIGKAA
                     GVQISKQSGTPGTGVRVRIRGTNSISASNDPLYVVDGIPMIPESNSKVNASEDFPDYL
                     NTKSNSITDINPADIASIEILKDASATAIYGSRGANGVVL"
BASE COUNT         1024 a          541 c          640 g          796 t
ORIGIN      
        1 gaaaatttga taatcacaaa aacatgatag ccaacaaaga actttttaaa gcaatagatc
       61 gggaaaactt actcctgata aggaccgaaa tccaaaacgg tgctgaacca aatgaaaaga
      121 acggttatgg tgatacggcc atgcatttta gtatcaccaa gggtaacctg gaaatattga
      181 aattattatt ggagctggga ggagatataa atgcaaaaaa tcaggaaggc gtgtcacttc
      241 tgcttcagag tatcggagaa gagaaatttg aactgtctca ttttcttatt gagaataaag
      301 ctgacgtaaa tcttcaaggt aaagagagcg agtctcccat tcatattgct tcaagattag
      361 gtaacaatgg acttgtaaaa aaattaatac aaaagggtgc tgttgtaaac gttacggata
      421 acagactgtg gactccatta cactacgttg gctatcttga caatcccaat cttgaggtgg
      481 ttgaaacatt gattgaaaat ggcgctgacc taaatgctaa agataacggt ggcagaacct
      541 gtttgcatta tgttgcaatg aaggaaggta agccaaaaga catcaagcta gccgggctaa
      601 tcactaacca gcaaaacatt tatattcaag ataacgatgg aaatacggct cttcattttg
      661 cagctgaaga cagcagcttg aaaatggtgg aatgtctcat tagcaaaggt gcggatctga
      721 gcctccggaa ttccaggggg gaaacacctg ctggacttgc ggaattagcc aacagaaaag
      781 caatcaggaa ggttttgaac agtggaaaat aataaatgag agaggaactg atcttatgga
      841 aacaaaaact ttttttctgt tgttgcctgc acatatgcag taactttctt caattctggt
      901 ctgtgaaggt aaagacctag gcaaagaaaa attatttttc aaaattgaca aaggcaaaaa
      961 taagagtgga gaccaaaacc actcaaaaaa tggggtgaac tggaaaacca aacgactacg
     1021 ggaaaatcta aaattttaga aacaatgaac aaagttaacc aagcccgtga aataataaga
     1081 aaatatccct ttcaagaaaa acaattgagc gattatgcaa aggacaaaat tggattatac
     1141 tttcgcaaag aatcaagggg ctattattat aatgggaaga ttaccaaact atttaaagtt
     1201 ttaacacctg aaatcttggc agagagtttc cattttatta tcaatttagc tgatacatca
     1261 ccaacattaa aaatgggata ttctaaatta ttcggctcac ctcatttgcc cggtgactgg
     1321 aaaatgcctg gtgattggaa aggcatgtca ttttggtttc agataaacct ggaagaagtt
     1381 aaaccttttg ataaagaaga cctgctccct gaaaaaggga tcatctatgt ttttcagcac
     1441 tcctcagaag gaaaatgtaa aatatatttt tatacagaca cgttggacaa acttataacc
     1501 agagcagaca ttcctgttct caactacgaa aaagagcaaa cgctttcatt tgaaccttca
     1561 ttcatttttt acataggcgg tgacgcgtat gactatagtg aaatctataa cttcattcct
     1621 aaagcgctta gagatgattt agagaagttg ctggagtgca aaatggatga caccgattca
     1681 tgcacgcgtt tatttggcag gccagtttat tggcagggtg aagatgaaga atttgatgag
     1741 gaatttgatg aggatcaaga tttcgaaagc aatgaacttg ccttgttctt tcaagatgaa
     1801 ttagatgatg cggctattaa tatatgggtg ccaaaatcag atttgagaaa tggtgttttt
     1861 gaaaatgcat ttgaaacttt ttccaccact taataaaaaa attggtaaga gaaccgcgta
     1921 ttaacaaatg catctttaat caatgatgaa agatcgggtc tgaagtcgga ttcatggcgg
     1981 atgtcattcc aggagtcaca ggatctccca ccgctgcaaa atgcaagaac catattggaa
     2041 gaaagcgacg agccactaaa catccacttg cagtctgggg aaacttttcc atgttgactg
     2101 aatcctgcaa atgccatgac cattaaatat agatccggct tgttaccata ttaccagatt
     2161 ttgggagaat tgaccaccta attaacaggc attcaacata tccttgcagc caaaaaaaat
     2221 tatcacaatg aaaagaaacc ttgtcatttt atttgttttt tcggttgttg tttttcaatg
     2281 gtctcctcta cattctcaaa cgacaaatac atcaattgaa aataaaggta ttgttactgt
     2341 aaaaggcctg gtaacagatg aggattctca aacaggtgta cctggcgtta ccgtgatgct
     2401 caaaggaacg aacacaggca ctgttaccga cagagatggg aaatatgtaa tcaatgcgtc
     2461 cgagagtggg ggagtattga tattttcttt tgtgggttat gccactcagg aaattgagct
     2521 aggcaatcgg agcgtaattg acgttgtgct aaagccagac acctacttat tggaggaatt
     2581 tgtcatagtt ggctatggag agcaacgaaa atctgatata acaggctcca tatcttcaat
     2641 cgaagtcgaa aacctcgaca gattgccggt tgcaagtatt gatgatgcca tgatcggtaa
     2701 agctgcggga gtgcagatct caaaacaatc aggaaccccc ggcaccgggg ttcgtgtgag
     2761 aatccgggga acaaactcaa tttccgccag caacgatccc ttatatgtgg tggatggcat
     2821 tcctatgatt cctgaatcca attcgaaagt gaacgcgagc gaagacttcc ccgattacct
     2881 gaacaccaaa agcaattcaa ttacggatat taatccagcg gatattgctt caatagaaat
     2941 cctaaaggat gcgtccgcaa cagcgatcta tggatcccga ggcgcaaatg gcgttgttct
     3001 c
//
LOCUS       DASWHX010000041        27248 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_3353139, whole genome shotgun sequence.
ACCESSION   DASWHX010000041 DASWHX010000000
VERSION     DASWHX010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 27248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27248
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3353139"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="VGA21_02290"
     CDS             <1..193
                     /locus_tag="VGA21_02290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649365.1"
                     /translation="RNNDRLYGWTSMWMNYVYVLLPNNGKTTGLQANLNNLSDTENAV
                     LEHIKISLWLQHESICSWA"
     gene            168..1784
                     /locus_tag="VGA21_02295"
     CDS             168..1784
                     /locus_tag="VGA21_02295"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX9649366.1"
                     /translation="MSQFVLGPNLSNNDGANVDPVILWIVGALAFVVMLSAAFNYTNL
                     SIARSIRRGREVGVRKVIGANRGNVIWQFISESIVISLLALLLSAGVFILLRNQFLTI
                     DPELLEIVSLKISPGVTVGFILLAIFVGFISGILPAIFFSRISPVKVLKNAASIKGFR
                     HLNFRKALITIQYTVSLMFITAAVIGFNQYKHSLAFNLGFTTDNILNVRLQGNGAAIM
                     RKEFNEIPEITRISGSNLVPSIGNFWSASLKYQDPNDSIFVYFVQVDENYIPLHDHKL
                     IAGRNYIGKSGKFEESEIIINEKTLERFNIAGADPYKAIGEYMKLDGKDVVIIGVIKD
                     FHYGKIDTDLLPVIFRYSNDQDGYSDGYLNLKINSTDLPGTMAKIENAWKKVDTVHPL
                     NAKFYSEQIENAYGEYSAMVKVIGYLAFLAISIASMGLFGMVVFNTESRQKEIGIRKA
                     LGAGILNIMYLLSRGFLILVGFASVIALPATYLFFEKIFLSKIPYHSPIGFTEIFLGL
                     GIVGAIALLMIGSQTYKIARVNPSTVLKNE"
     gene            1972..2982
                     /locus_tag="VGA21_02300"
     CDS             1972..2982
                     /locus_tag="VGA21_02300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649367.1"
                     /translation="MASNQEIQNNFFQVLKTRIPTNHSFVDEISEALNLSTDSAYRRI
                     RGETLLTLQEITVLCKKYSISLDALLGNSTYSVTFDYYPIDNERLTLRKFLEMVSNDF
                     KEADKAGSVEVIYLARDMPIFYLFQFPEICAFKIYFWNLLYQDPNHKTEKFNLDQVDK
                     NSIDLCRFIWDEFVKVPSIEIWGVPTFDAILDEIDFLWESLLFESKDIALRVCDKVTQ
                     LLSHVKKQAELGYKFSDGNPVGGKPGNFQLYYTDFQLTHNKIMINGDIKRIYISQSLD
                     TIITTNPFFFERNYASISNALKRSTLISRVGEKERNQFINKCQRKIDILRNKIQMSDD
                     FT"
     gene            complement(3015..4691)
                     /locus_tag="VGA21_02305"
     CDS             complement(3015..4691)
                     /locus_tag="VGA21_02305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649368.1"
                     /translation="MVKSKNFLSVLILFLPLAAFAQDLSKVGKSKAFRISGGISLNNI
                     YNSANAQNPFSYYLGANLNFDFYGWSVPLSFSYSNREHSFTQPFNQYGLSPTYKWITM
                     HLGYRSLNFSNYSYAGILFKGAGIELSPDIGLKFTGFYGQLQKAIQPDSLNQTEISFQ
                     RLGYGAKLEYSFSGHSVNVSLFKAYDNFNSLEYIPQNSNVTPHENLVFTVGGASTPIK
                     GLNINFEWASSAFTEDIRSEAVEEPKSWWRHAGKLLTNRESSGYYWAGRMGLNYTFLK
                     SIVGLNYEIINPGYRTLGQYYTNNDIETLSLNFGQSVFKDKVNLTVSAGQERNNLNNN
                     EVEETSRFVFASGININFSQKVQGNGSFSTFQSYTRVRPLFDELNVVPGEPVDSLNFT
                     QVNKSANLSASWTISKSENKQQNLNAIFNYQGGVNKGSEDAPNSLGFTESISYRVNWV
                     EKESTLSLSLNSNQNSFGELKTVLLGPTASLGKIFFGKKLRSMITMAYNTSVSGGHSA
                     SSTFFARITNQISLKKKHNINSMFGYNVRSNAMNHKNGDISLSAGYSFSF"
     gene            complement(4791..5582)
                     /gene="arsM"
                     /locus_tag="VGA21_02310"
     CDS             complement(4791..5582)
                     /gene="arsM"
                     /locus_tag="VGA21_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018477585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HEX9649369.1"
                     /translation="MSNIKEIVKENYAGIVKENKSNGCCGPTCCGPEDSTLTEFSEDY
                     SNLEGYVQDADFGLGCGIPTSGAGIKEGHTVLDLGSGAGNDVFVARAIVGETGNVIGV
                     DFTPEMVRKANENKEKLGFTNVQFVLGDIESLPFQEKMVDVVISNCVLNLVEDKRAAY
                     SEVYRVLKTGGHFSISDVVVTGPLSKKMQSITELYAGCIGGAIPKEEYLRIIKEAGFT
                     NVEVNKERVISLPEGFLSNYLTADELEEYRKLRVEILSITVTGHK"
     gene            complement(5661..6200)
                     /gene="sigZ"
                     /locus_tag="VGA21_02315"
     CDS             complement(5661..6200)
                     /gene="sigZ"
                     /locus_tag="VGA21_02315"
                     /inference="COORDINATES: protein motif:HMM:TIGR02959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor SigZ"
                     /protein_id="HEX9649370.1"
                     /translation="MSTEQLWLQFHSKLKSFIYKRVAERAAAEDILQDVFLKIHLKSN
                     ELDKVNNLQGWVYTITQNQIIDYYRKSKIQSTVTEDFFVPEERTEHQHQELAKCLVPF
                     VNELPQKYKTALLKTDLGGVSQKKFAEEAGISYTGAKSQVQRARKQLKELFLNCCTIH
                     VDKYGTVTDYYDRDHCENC"
     gene            complement(6264..8519)
                     /locus_tag="VGA21_02320"
     CDS             complement(6264..8519)
                     /locus_tag="VGA21_02320"
                     /inference="COORDINATES: protein motif:HMM:NF013123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX9649371.1"
                     /translation="MKNFILVFFVFFITKVFSQATITIEDYNRAVSYLWQNLNDKRVF
                     NLRVMPSWFSDSAGVWYTTQEKNYKFFNKIEFSAMTIEPLFDQEKLAQILTDSLNKEI
                     SAGDLPITNINYIDNQHLEFSLIGKRYLLDLTNYTLTEKEESPRNLLESKSPDGKWIA
                     YVNDYNLFIKSTENGEVKQLSTAGKKNYEYASYYGWGDIITGEGGERPERFSVNWSPD
                     SKYLQTYICDLRSADKMYLLDWSVDTLYRPRLLSYYRGSPGDTAMVYMIPVLYEVSSG
                     KEIKTNLPRNTHINQVNFRWSEKPGIVYASYSERGFKRIHLLQMDLNNSNLTEIYAEE
                     SETNIDNFSYFLAEKTGKIIITSERSGWKQLYVLDLKTKELMSITNGDYYIDDVEFIN
                     EEEGWLLFLASGKQAGINPYFQQLYKISLNGKGLKNMTADDLHHEISISRDGNYFFDN
                     ISSVNKATRTVLRDTKSGKLLKEISSADIEEIVKENWQFPEIFSVTARDGKTTIYGAM
                     WKPVNFDPAKKYPVIDQTYTGPHTNMFPRSFARGLTISNQALAELGFIVVTVDGLGSA
                     GRSKEFHNYSYKNMGKNLTDHVIAIKELGRRYSWIDTDKVGIFGHSAGGFDAGHAVLE
                     FPDFYKVAVASSADHDFRMEKAWWPEMYMGWPVDSTYHEVSNITMAANLKGKLLITHG
                     GIDENVNPSATFKLAEALVKANKEFDMLIFPSQRHGYSGDYGRYFTRKKWNYFVEHLL
                     GARPIWDFEME"
     gene            complement(8560..9015)
                     /locus_tag="VGA21_02325"
     CDS             complement(8560..9015)
                     /locus_tag="VGA21_02325"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX9649372.1"
                     /translation="MSNDARVERAMPYDLPILNQISVESKMHWGYPLDWLQNWVETLR
                     ITRKFLEENHVYKISTDRIIGFCAIVKKPDAYEVIHLWIAPKYIGKGYGKKLLQETLQ
                     IVVMEKSEVIVESDPNSEKFYQSQGFKTFGQRESYPKGRFLPLMKRVFP"
     gene            complement(9143..10015)
                     /locus_tag="VGA21_02330"
     CDS             complement(9143..10015)
                     /locus_tag="VGA21_02330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649373.1"
                     /translation="MIRIRKYLVILVLIFLSASIAMPQEKAAVRVDLSFHQLNNGPPT
                     LTALIRTRVDRRYQPVENVKVKFYHSEMDELNLLGSLATDSDGKANLQPGKEFNSLWD
                     TTSFFTFIASVTDDPNFEDNDDNIEITKAKITIELLEGEEKEVKVQINKYLEDGTIEP
                     AQEVFVKLQVKRTFGTLSIGEDTYETDDEGVFTASFPEDIPGGPDGILTIMAFVEDDD
                     NLGNLKMEKEAQWGTIVSESDDSFGGRSLWASRDKAPIWLLVFPNLIIISIWGTMIYL
                     VMLIFKIKKIGKTP"
     gene            complement(10002..11306)
                     /gene="nrfD"
                     /locus_tag="VGA21_02335"
     CDS             complement(10002..11306)
                     /gene="nrfD"
                     /locus_tag="VGA21_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014771926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HEX9649374.1"
                     /translation="MNSEKIARDLMPVKFGKAGKIWVYILLAIAAFGLYAYTKQLRFG
                     LGITAMRDYASWGIYISNFVFFVAISLVGSLITAVLRLTNANWSTPLTRIAEIIAVAA
                     IVFASLIIVIDMGRPDRLLHLFAYGRLQSPIIWDVIVITTYLFISLLLLYFPLIPDLK
                     IMQKYNPPIPGFIRKIQKWMNPNWVGSADQKKLVEKAIKILSIIIIPVALAIHTVTSW
                     LFATTYRPGWDSTNFGAYFVSGAFLVGAGGVVIAMYALRRTYKLEDYITEKHFDHMGK
                     LLVLLSLLYLYFNINEYLVPAFKMKKPEVEHLTGLFTGDYAAIFWPVILLGMVIPVIV
                     LLFPKGRRPVPMFIIGILVVAGAWFKRFLIVTPTLLHPFLPMYDVPGNYLHYSPTWEE
                     WSITIGSLAGALLIVTFFVRYFPIVPIAETIHERNTKNHDQN"
     gene            complement(11315..12235)
                     /locus_tag="VGA21_02340"
     CDS             complement(11315..12235)
                     /locus_tag="VGA21_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014771927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX9649375.1"
                     /translation="MEEKHNQTRREFLKGSLLSGVGAVSLAENPFSPLLEENVKESGE
                     IVKLLSDDGHVIEIDRAYLKPVPETQHVHNSDARKGIPGKKFVMVIDLSRCKNLKKCQ
                     AACNKMHFVTEGQNWIKIYPMQDAPHTAPYWQPTTCMHCDEPPCVKVCPVDATFKRED
                     GLVLIDNERCIGCRFCMAACPYSTRVFNWDEPVLPLEVLQREYSSETSVPPKIGTVNK
                     CDFCPDMAREGKLPHCVSACPNGVFFFGDLNEDTVTNGAETFRFSQLIKDKAGYRLME
                     DLGTKPRVYYLPPANRLFPFEEGLEEEKKN"
     gene            complement(12247..12486)
                     /locus_tag="VGA21_02345"
     CDS             complement(12247..12486)
                     /locus_tag="VGA21_02345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649376.1"
                     /translation="MEFLDKKRSNLRRKYLKWILGSAAGIAIAWIFSIRRKPEVVKML
                     TKDGKLVEVPLDRLPKKTHKVNLKEMQTWIWKHNT"
     gene            complement(12474..13349)
                     /locus_tag="VGA21_02350"
     CDS             complement(12474..13349)
                     /locus_tag="VGA21_02350"
                     /inference="COORDINATES: protein motif:HMM:NF040196.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5777 family beta-barrel protein"
                     /protein_id="HEX9649377.1"
                     /translation="MKNQTNKNLSGGVKLLILLPILISNSLFAQEEEGTDADNRPVRP
                     TFESALLMDLQSVMVPNHKTLEFDIQHRFGTVEEGITDLFGIYAPANIRMGFTYTPIN
                     NLAVGFGFGKFKKYLDLSLKYAILKQRRDWSIPVSLTYYTNIARDSRDKSLFSKPVHR
                     FSYHHELIIASRLSSKLSLQVAPSFSHYNAIDSLYSNDIIAISAGGRFRFSSQGAILI
                     NYVQQITNHDDPNFDLKPNITIGLEFSTGSHAFQVFVSNYQGILPQENNTFNEYDFTK
                     GEFLIGFNITRLWSF"
     gene            complement(13363..13737)
                     /locus_tag="VGA21_02355"
     CDS             complement(13363..13737)
                     /locus_tag="VGA21_02355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649378.1"
                     /translation="MKQGIKSIMAIIFFLGTIIYSCTYDEIIPQEVEVPENVSFSIDV
                     QPIFTKSCALPGCHSAGGQQPNLTIGNAYNSLTFFGYVNLADPANSKIYQQINIGKMK
                     TYATDQDRAIILKWIEQGALDN"
     gene            complement(13739..14995)
                     /locus_tag="VGA21_02360"
     CDS             complement(13739..14995)
                     /locus_tag="VGA21_02360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649379.1"
                     /translation="MKSRLKIFMIFPALLAGCTYFENEPPPKPFVPKATTSLEAVYTT
                     TIPNSITAPFWNTLNYYEVTATTITSGEVPTDDGLFNMSGTINGTSSFNEGIDPILEL
                     KAGYDDSYLYILASWQDETYNASNGSWFLNGPSDPDKSGSTTGWTSQQNDDNIIFVFP
                     VSSNESDVWKWSLALSEPLGYALDLFNSGTGNQFDAGDKIFIRNAVDPEDNRSGPMLD
                     WDGIDNQQLDRSPGGLTFLDPGYYLLNTKPLTGDAELGDMVFQTTCSSCHGVNAVGGE
                     AKALNDRARFMRQTFTAFQAFVSSGNHDGSSYYSGLTEEERVNLYARLKAYSGIPGYL
                     IQNPTGSNSDISALSNVQLALIDEKASNGGYVVLLIRQLNTGNLDDIVFNPDNSPYTF
                     HVYLSDNDDLNKIGEMNIELTFKPKN"
     gene            complement(15050..16165)
                     /locus_tag="VGA21_02365"
     CDS             complement(15050..16165)
                     /locus_tag="VGA21_02365"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEX9649380.1"
                     /translation="MKTHRILWVSFLSTLLLLLTVRCSDDDPAPLLSISGTVTYPGIS
                     GNTAAGGSVVLLAKTANALEYNFTSIADASGNYIFDNLEAGTYYLNANYYTDNQNLGA
                     RLEGVNFNAGDDIEVVIGGVDVTENITLVSVGQTMASAMDINYDGREGGAGNATEWEF
                     DPNHTTVDFAFDFKEENSEFTGSFGRVTKLILNLDPASLSTSSLMAEVDLMSVNTRTR
                     GGRDPLWDGEFNALTESTVFTETNCIATTFGIFADATFPATVTDPQRYASFTSTSVEP
                     YGDGFIAKGNLSFSAVMSTTPSSPYGITNNPNTMVTKAAQIVFKYRTGTEDATRFYAS
                     FEGKMIMNAKADFNIFSSNIVDEPIKVYVNIQLRKNK"
     gene            complement(16274..16777)
                     /locus_tag="VGA21_02370"
     CDS             complement(16274..16777)
                     /locus_tag="VGA21_02370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649381.1"
                     /translation="MRPYAILLVIAASCITSSSIAPEVIFYYRIEIINPDPAANIIRQ
                     ELEQSFPLRKYAITNSSGNTKPFYQEFELNYDLAIDSLMPPHTFVHKMQPFSEAIIPE
                     TDSRLLKIHLFPEKEPSYSMEIYLWENSSWNFLAQTGIHPINSEESEYQELTEKFRES
                     IIQFSFK"
     gene            complement(16803..17204)
                     /locus_tag="VGA21_02375"
     CDS             complement(16803..17204)
                     /locus_tag="VGA21_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014771924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX9649382.1"
                     /translation="MKKFKLKYLLFIVAVYFMFSFTVFQEKWEVPAKYESMKNPTEGD
                     KENLAIGKQLYDKHCRSCHGKEGLGDGTKAGELETPTGDFSAEDFQKQSDGALFYKTT
                     TGRGDMPEFAKKIGSDEDRWLIVNYLRTFAE"
     gene            complement(17381..18145)
                     /locus_tag="VGA21_02380"
     CDS             complement(17381..18145)
                     /locus_tag="VGA21_02380"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009579297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HEX9649383.1"
                     /translation="MEILIFFIAHWYLSLFFQTFFLHRYAAHKAFTMSPVKEKIFYIL
                     TWIFQGSNYLSPYGYGIMHRMHHAFADTPNDPHSPKYDESIFKMMWKTKTIYAGFSNK
                     QVKPDPRFTDGIPDWHAFDRIAKSWPSRILWGTAYVIFYFFFATAWWMWLLLPIHFLM
                     SPIHGAIINWFAHKYGYRNFEVGDTSRNFLPVDFLMMGESYHNNHHKNGSRANFGFRW
                     HEIDPTYLVIKILDKVGVIKLAKQQGISLEAAKKAA"
     gene            complement(18240..18839)
                     /locus_tag="VGA21_02385"
     CDS             complement(18240..18839)
                     /locus_tag="VGA21_02385"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX9649384.1"
                     /translation="MNQTQVFLTYQTLLHSIALQILRCKADAEDAVHDAFERWLSVDD
                     NKVQNAKAYLTSMVRNISINKLRRFSFRKTESLEAVPEPHKGHIEPDLSHFDLDIDIG
                     KALSIINHKLEPLERAVVLLREVLNFDYEVLTKIVGKKADHCRQILSRAKKKLGNSKM
                     SFQINYKSNFLAEIFRKSCQSGTFTDLINYLKADLPAKK"
     gene            19181..20650
                     /locus_tag="VGA21_02390"
     CDS             19181..20650
                     /locus_tag="VGA21_02390"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX9649385.1"
                     /translation="MSKLQDFILILSATLGVFIESSFSPKFVFGIFFDIIFWFTISGL
                     YITYFWNKGSKNLRKYFLHTIYILITLFAVLFQLKNPGSGVAQIITLLTLFISGSIFL
                     NVFRQTLFLVFAILLRFGFSEFFAPMDPYFHYSLIVIVIISIVSGFFLINFEKTIRDA
                     NALFMESNQMLIELDQIYAPKYNNAGFHSHPGFEENEVMFVPPTLEIMPGTGHNSNGK
                     SVILDYPNLKVESAKKVKNDYKSELQLPFLINCLRKDLAREGITLKTQLINEFKFQDL
                     KMRCGEMVDHLHDFFLSEKEFNSGKIVIDNTTTFNEFIISSEFNWQREFSLAGIPSDL
                     RSAVYDLGGKCFYNSDQNENSRLIICLPNPQVIPGSKFSILNLHSLTDLVFVNEIINA
                     MDRFLCSLDFSVENLSRELGTSRMQLHRKVKGSFGMSPGSLFQYLRMKKAEFLLKSGY
                     SRISDIAYSLGYCDQSHFSKAFRAWSGYPPVEYMRKNQA"
     gene            complement(20696..21595)
                     /locus_tag="VGA21_02395"
     CDS             complement(20696..21595)
                     /locus_tag="VGA21_02395"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008198916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEX9649386.1"
                     /translation="MKSFSPKDLSLSEFHSYLLGAVTPRPVAFASSVDNQGNVNLSPF
                     SFFNAFSANPPILVFSPSRRGKDNTTKHTYENIREVPEVVINIANYNLVEQMSLASSN
                     YAKGVNEFVKAGLTEVKSAMVKPPRVLEAPIAFECNVNEVIQLGDKGGAGNLVICEIL
                     LMHITEEILDENEKINPVKLDAVGRLGEDWYCRVIPDSIFRVPKPTQHLGIGFDSLPD
                     EIKKSPVLSGNNLARLAGVKELPNKEFLEQIKNSVEVSDVFTRFINDKESMEYHLHML
                     GKAALERGETDLAWAYLLASKSN"
     gene            complement(21602..22873)
                     /gene="fahA"
                     /locus_tag="VGA21_02400"
     CDS             complement(21602..22873)
                     /gene="fahA"
                     /locus_tag="VGA21_02400"
                     /EC_number="3.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009582057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004334 - fumarylacetoacetase activity
                     [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetase"
                     /protein_id="HEX9649387.1"
                     /translation="MRNPNDAKLKSWVEVPKGSDFPIQNLPFGIFSTGTRNKRAGIAI
                     GNQIVDLSELAKRGYLDIPGIDTAVFSNDILNAFLEVGRKKCGEVRMKVSELLDSENS
                     ILRNNENEIREVFVNMADAQMHMPINVRNYTDFYSSKEHAINIGTMFRDPENALLPNW
                     KHLPVAYHGRASSIIISGQDIYRPKGQTKSADDPRPIYGATQRLDFELEIAFVACKSN
                     ALGDQITLANAEDYIFGFCLFNDWSARDIQAWEYVPLGPFLSKNFASAISPWIVTLDA
                     LEPFRVDGPTQEEKVLPYLAFEGSKNFDINLEVFIKPDKSEEVSVSKSNFRHMYWNVN
                     QQLAHHTVNGCNIQIGDMYASGTISGPTPDTYGSMLELAWQGTKPIRMKDGSVRKFIN
                     DGDEVILRGYCEKNGIRVGFGEVRNRVLPAK"
     gene            22992..25619
                     /locus_tag="VGA21_02405"
     CDS             22992..25619
                     /locus_tag="VGA21_02405"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018473261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEX9649388.1"
                     /translation="MSVSTKYDPAKVEDKWYKVWLENGYFSSTPNPDKEPYCIVIPPP
                     NVTGVLHMGHMLNNTIQDILVRKARMEGKEALWVPGTDHASIATEAKVVAMLKEQGIS
                     KKDISRKEFVEKAWEWTHKYGGIILEQLKKLGASCDWSRTRFTMEDDLSKMVIMTFID
                     LYNKGYIYRGYRMVNWDPIGKTALADDEVNHRDVNSKLYYIKYKIENSQNEFITIATT
                     RPETILGDTAVCINPADERYKNLAGKRALVPLINRSIPIIDDDYVTMEFGTGCLKVTP
                     AHDVNDYELGKKHKLETVDILNDDGSLNKSAQLFVGKDRFEARELIVNQLEKEGHLVK
                     TEAYVSSIGFSERTDAIIEPRLSMQWFVRMNDLVKPALENVMNDEIKLYPPKFKNLYR
                     HWMENVRDWCISRQLWWGHQIPAYYYTDNEFVVAETPGKALELAQEKTGNTKLKPSDL
                     KQDPDVLDTWFSSGLWPISVFNGLLDPQNNDIKYYYPTNDLVTAPEILFFWVARMIIF
                     GYEYKGEPPFRNVYLTGIVRDKLGRKMSKSLGNSPDPIELIKKYSADGVRTGMLFSSP
                     AGNDLLFDIKLCEQGRNFANKIWNAFRLVSGWNVDNDGFSTPNQIAIDWMNSRFNQSL
                     EELESHFRKFRISDALLTVYKLVWDDFASWYLEMIKPEYGKPIDSQTKQETIRILENI
                     LKILHPYMPFITEEIWDQLGERDNKSRIIISPWPAGGKIDQQIINKANHSFDLITAIR
                     KARNSRGISLKESFTLQLLNGDSQVGEFEIFIKKLANVSSFEKIPKSDSDGISLVVNN
                     VDYFIPLTGKVDKESEKKTLLVELKYAKGFLESVNKKLSNERFVNNAPESVVEVEKKK
                     KADTESKIRSLEESLSRLN"
     gene            complement(25910..26302)
                     /locus_tag="VGA21_02410"
     CDS             complement(25910..26302)
                     /locus_tag="VGA21_02410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649389.1"
                     /translation="MKILILTLIPTLSFAQCNLIADEKDIAGQHLLMLREYNLIFPLD
                     SLENKIMEIPVGIIMKHDSLEWLWMPLEMKINASGGFFTTYISFVDPLTGKREFCKIR
                     NTNMNYFKQIEKNTIELLRNLSLPLDEH"
     gene            complement(26310..>27248)
                     /locus_tag="VGA21_02415"
     CDS             complement(26310..>27248)
                     /locus_tag="VGA21_02415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649390.1"
                     /translation="QYGFLGFQEGSPIFVSRAGRKVNSYKSIIINEKVEKIEIFNLRF
                     TPPFETSNIETGFLRLIDFGRSSWFYKVMFSNDDFTIYYQNLWLQHNGKDYFLDSIYN
                     ADKKIHSSFSSDGKYLFVNTLNTLSDYYNPAQDNRIMVYDLSEIEQGKIGKQYIPCTH
                     CSDSYLIGDQLFFTIGRKDGYGGYSNKDIYLAPWGNLEDSVKVAANTDIIAISPDGKY
                     ILGTRFWDRQKTTAVIVDVEHKKYQMLLGRDYAERKTFYSYYKSKFAFDFKGYLIYVD
                     FPEGYPFDALKWRNEEIPDWTEKEFWQQFQHPPLEK"
BASE COUNT         8103 a         5484 c         5135 g         8526 t
ORIGIN      
        1 tcggaataac gataggttat atggatggac cagcatgtgg atgaattacg tgtacgtatt
       61 attacctaat aatggtaaaa ctactggttt acaggcaaat ctcaataatt tatctgatac
      121 tgagaatgcc gtacttgaac acattaaaat atctttatgg ctgcagcatg agtcaatttg
      181 ttcttgggcc taatcttagc aacaatgacg gagcaaacgt ggatccggta attttgtgga
      241 ttgtcggtgc attggctttt gtagtgatgt tgtcagctgc tttcaattat acaaatctgt
      301 ccatagcgcg atctattcga agaggccgtg aggttggagt aaggaaagta atcggagcga
      361 accgcggcaa tgtaatctgg caatttattt ctgaatcaat agtaatttca ctcttagcgc
      421 tactgctttc tgcgggagtt ttcattctgc tcagaaatca atttctaacc attgatcctg
      481 agctattaga aatagtatca ctcaaaataa gtccaggtgt aacagttggt tttattctcc
      541 tggccatttt tgtaggcttc atatccggca tacttcctgc aatatttttc agcagaataa
      601 gtcctgtaaa ggttcttaaa aacgctgctt caataaaagg attcagacat ctgaatttca
      661 gaaaggcttt aataactatt caatacacgg tctctctcat gtttattacg gccgcggtta
      721 ttggattcaa tcaatacaag cattcgctgg cctttaacct tgggttcacc actgacaata
      781 ttttgaatgt aagacttcag ggtaacggcg ctgcgataat gcgaaaggag tttaacgaga
      841 ttcctgaaat cactcggatt tccggatcaa acctggttcc cagtattggt aatttctggt
      901 cagcatcatt gaaataccag gatccgaatg attctatttt tgtatatttt gtccaggttg
      961 acgagaacta cattcctcta catgaccata agttgatcgc gggaagaaat tatatcggaa
     1021 aatcagggaa gtttgaagaa agtgaaatca taattaatga gaaaaccctg gaacggttta
     1081 atatagctgg tgctgatccg tataaagcaa ttggagaata catgaaattg gatggaaaag
     1141 atgtagtaat aattggtgtg attaaagact ttcattatgg gaaaatagat actgatttat
     1201 taccggtcat ctttagatat tcaaacgatc aggatggata cagcgatggg tatttaaact
     1261 tgaaaataaa ttccacggat ttacccggaa cgatggctaa aattgaaaat gcctggaaaa
     1321 aagttgacac cgttcatccg ttgaatgcaa aattttatag cgaacagatt gaaaatgcct
     1381 atggagaata ttccgcaatg gtaaaagtaa taggatatct ggcttttctt gccatctcaa
     1441 tcgcatccat gggacttttt ggcatggttg ttttcaatac ggaaagcagg caaaaagaaa
     1501 ttggaataag aaaagccctt ggggccggca ttctcaatat aatgtattta ctcagtcgag
     1561 gttttctgat tctggttggt tttgcttcgg tcatcgctct gccagccacc tatttatttt
     1621 ttgaaaaaat attcttgtca aaaatacctt accattcacc tatcggtttt acggaaatat
     1681 ttttgggttt aggaattgtg ggagccattg ccttgctgat gattggctct cagacttata
     1741 aaattgccag ggtaaatcca tcaactgtat taaagaatga gtgattaact tttcggttcg
     1801 aattagattc gggcctggat ttcaaaaggg tgggaacgaa ctttaggttc aaatctaatt
     1861 tcatttatct cattgggaat tgtatatttc ccttgccgcg tattcttact tactaattat
     1921 caccgctttt atctatgtta ttaacacaaa tttcttcaat ttgaggcaag catggcttca
     1981 aatcaagaga ttcaaaataa tttctttcag gttctcaaaa ccagaattcc caccaatcac
     2041 tcttttgtcg atgaaatctc agaagcttta aatctaagca cagacagtgc ttaccgaagg
     2101 attcgtggcg aaacacttct gacacttcag gaaataactg ttctgtgcaa aaaatatagc
     2161 atttccctgg atgcgctgct gggtaattct acttattcag taacttttga ttattacccc
     2221 attgataatg aacggctcac tttaagaaaa tttcttgaaa tggttagtaa tgactttaaa
     2281 gaagcagata aggccggaag tgttgaagtg atatacctcg ccagagacat gcccattttt
     2341 tacttattcc aatttccgga aatatgtgca ttcaaaattt acttttggaa ccttttatac
     2401 caggatccaa atcataaaac cgaaaagttt aatcttgatc aggtggataa gaactccatc
     2461 gacctatgca gatttatctg ggatgagttt gtgaaagtgc catcaattga aatctggggt
     2521 gttcctacat tcgatgctat acttgacgaa attgactttt tatgggaaag tcttctgttt
     2581 gaaagcaagg atattgcttt gcgggtgtgt gacaaagtaa cgcaacttct ttcccacgtc
     2641 aaaaaacaag ctgaattagg gtacaaattt tcagatggca atccggttgg cggaaaaccc
     2701 ggtaactttc aactatacta caccgatttt caattaacac ataataagat catgattaat
     2761 ggggacataa agcggatcta tatctcgcaa tcattggata caataattac aactaatccg
     2821 tttttctttg aaagaaatta tgcctccata tcaaacgctt taaagagatc aacgcttata
     2881 agcagagttg gcgaaaagga aaggaaccaa tttattaaca agtgccagag aaaaatcgat
     2941 attctgagaa ataaaattca aatgtctgat gattttacct gaatgtaagc ggaagggcat
     3001 taatcaggga atcgttaaaa agaaaatgaa taccctgcac tgagggaaat atctccattc
     3061 ttgtgattca ttgcgttaga cctgacatta taaccaaaca ttgaatttat attatgcttt
     3121 ttcttaaggg atatctggtt tgtaatcctt gcgaaaaaag tagaagaagc tgagtgacct
     3181 ccacttacac tcgtgttata tgccattgtg atcattgatc taagcttttt tccgaaaaaa
     3241 atctttccca gagatgccgt tggtcccaac aaaaccgtct tcaattcacc aaatgagttc
     3301 tggttagagt taagtgataa ggaaagggta gattcttttt ccacccagtt tacacggtat
     3361 gagatagatt cggtaaatcc caatgaattg ggtgcatcct cgcttccttt atttactcca
     3421 ccctgataat tgaaaatggc attcagattt tgttgtttat tttctgattt actgattgtc
     3481 cacgaggcag acaaattcgc ggatttgttt acctgggtaa aattcaacga atcaaccggc
     3541 tccccgggaa caacatttag ctcatcaaac aacggcctga ccctggtgta actttggaaa
     3601 gttgaaaagc ttccgttccc ttgaaccttt tgagaaaaat tgatgttaat tcccgaagca
     3661 aaaacaaacc gggaagtttc ttccacttca ttattattaa gattgtttct ttcctgtcct
     3721 gcactcactg tcaggttcac tttgtcttta aaaacagatt gtccaaaatt aagagataaa
     3781 gtttcgatgt cgttattggt atagtactga cctaaagttc tgtagcctgg attaatgatt
     3841 tcataattca gtccaactat cgatttaaga aaagtatagt ttaatcccat cctgcctgcc
     3901 caataatagc ccgaggattc cctgttagtc aacaatttgc ctgcatgtct ccaccaggat
     3961 tttggttctt ctactgcttc acttcgaatg tcttctgtga acgccgatga agcccattca
     4021 aaatttatgt ttaacccttt gattggagtg ctggcaccac ccacagtaaa aacaaggttt
     4081 tcatgtggag taacattact attttgaggg atgtattcca gggaattgaa attgtcataa
     4141 gctttgaaca ggctcacatt aactgaatga cctgaaaatg aatattccag ctttgcccca
     4201 taacctagtc gctgaaaact tatctcagtt tgatttaggg aatccggttg aatagccttc
     4261 tgcaattgcc cgtaaaatcc ggtaaacttc aggccaatgt caggacttag ttctatgcct
     4321 gctcctttaa ataatattcc cgcgtaactg taatttgaaa aattcaaaga cctgtaccca
     4381 agatgcatgg ttatccattt ataagttgga cttagcccat actggttaaa gggttgagta
     4441 aatgaatgct cacggttcga atacgaaaat gataatggca cactccagcc ataaaaatca
     4501 aaattaagat ttgctcccaa atagtaggaa aaaggatttt gcgcgttagc agaattgtag
     4561 atattattaa ggctgatacc tccggaaatt ctgaatgctt tggattttcc tacttttgat
     4621 aagtcctggg caaaagcagc caggggcaga aaaaggataa gaacagagag aaagtttttg
     4681 ctttttacca taaaaaagag cgtaagcatg aaactatcat gcaaacttct ctttcaatgg
     4741 tatttatatt cctaagtctg gtagtctggt aacatttgaa ggaaaaacca ctacttatga
     4801 ccggttacgg tgatactaag aatttccact ctcaattttc tgtattcttc cagctcatca
     4861 gctgttaaat agtttgataa gaagccttca ggaagtgata taactcgttc tttattcact
     4921 tcaacattag tgaaacctgc ttcttttatt attcgcagat attcctcttt tggtatggct
     4981 ccaccgatgc atccggcata tagttccgtg atggattgca tctttttaga caaggggccg
     5041 gttaccacca catcagaaat actgaagtgt ccgccagttt tgagtactcg ataaacttca
     5101 gaatacgcgg cacgtttatc ttcaactaaa ttaaggacac aattgctgat caccacatca
     5161 accatttttt cctggaatgg caggctttca atatctccca aaacaaattg aacattggtg
     5221 aatccaagtt tttctttgtt ctcattggct ttcctcacca tttccggtgt gaagtccacc
     5281 ccaattacat tgccggtttc cccaactatg gctctggcca cgaaaacgtc attacccgca
     5341 cctgatccca ggtccaaaac agtatgacct tccttgatgc ccgctcctga agttggtata
     5401 ccacacccca ggccaaagtc tgcatcctga acgtatcctt ccagatttga ataatcttca
     5461 ctgaactctg tcagagtgga atcctcagga ccgcaacagg ttggtccgca gcaaccatta
     5521 cttttatttt ctttaacaat gcctgcgtaa ttttctttta caatttcttt aatattactc
     5581 attggaattt tttgtttatg tttcttcttc catccataac gtttcaacta aaaaaaagac
     5641 gcaaggttgg caaatgcatg ctaacaattt tcacaatgat cccgatcata ataatccgtc
     5701 acggtaccat atttatctac atgaatcgtg caacaattta gaaaaagttc tttcagctgt
     5761 ttccttgccc tttgtacctg ggatttggct cccgtatagg aaatgcctgc ttcttccgcg
     5821 aatttttttt gggagacacc accaagatca gtttttaaca aagcggtttt atatttttgc
     5881 ggcaactcat taacgaaagg caccaggcat ttggcaagtt cctgatgttg atgttcggtt
     5941 ctttcttccg gtacaaaaaa atcttctgtt actgttgatt gtattttcga cttgcgatag
     6001 taatcaatta tctggttttg ggttatggta tacacccaac cctgaagatt attcacctta
     6061 tccagttcat tgctttttaa atggattttc aggaagacat cctgtaatat atcctcagcg
     6121 gccgctcgtt ccgctacccg cttataaata aaactcttca atttcgaatg aaattgaagc
     6181 caaagctgtt cagtgctcat ttttaaattc ttctcactca tttataataa cggcttcctc
     6241 tcaaaaaaga cgcaaagatt tcgttattcc atttcaaaat cccaaatcgg tctggcaccc
     6301 agcaggtgct caacgaaata gttccacttt ttccgtgtaa aatacctgcc ataatctccg
     6361 gaataaccat gccgctggct gggaaaaatc aacatatcaa attccttatt ggctttaacc
     6421 aatgcttcgg ccaatttgaa agtggcggag ggattcacat tttcatctat tccaccgtga
     6481 gttattaata gtttaccttt cagattagcg gccatcgtaa tatttgaaac ctcatgatat
     6541 gtcgagtcta ccggccagcc catgtacatt tcgggccacc aggctttttc catgcgaaaa
     6601 tcatgatctg cagaacttgc cactgccact ttgtaaaaat ccggaaattc cagcaccgcg
     6661 tgacccgcat caaatccacc tgctgaatgg ccaaagattc cgactttgtc agtatcaatc
     6721 caggaatatc ttctgccaag ttccttaatt gcaatcacat ggtctgtcag gttcttgccc
     6781 atattcttgt aagagtagtt atgaaattct tttgacctgc cggctgaacc caaaccatct
     6841 acagtgacca ctataaatcc aagctcggca agggcctggt ttgaaatagt taatccacgc
     6901 gcgaaacttc ttggaaacat attagtatgc ggaccggtat aggtctggtc aataaccgga
     6961 tacttcttcg ccggatcaaa gtttaccggt ttccacatag cgccatagat tgtagtttta
     7021 ccatccctgg cggttacaga aaatatttcc gggaattgcc agttttcctt aacaatctct
     7081 tcgatatcag ctgaactgat ctcttttaat aatttaccgc ttttggtgtc tcttaaaacg
     7141 gttctggtag ctttattgac gcttgatata ttatcaaaaa aataatttcc atcacgacta
     7201 atgctaattt catggtgcaa atcatcggcg gtcatatttt ttaaaccctt accattgagc
     7261 gaaattttgt aaagctgttg gaaatatgga tttattcccg cctgtttacc tgaagcaaga
     7321 aacagcagcc acccttcttc ttcattgata aattcaacat catcaatata ataatcacca
     7381 ttcgtaatgg acattaattc ttttgttttt agatccagaa catagagttg tttccatcca
     7441 cttctttccg aagtaattat tattttccct gtcttttcgg caagaaaata agaaaaatta
     7501 tcaatattgg tttcgctttc ctccgcataa atttccgtaa gatttgaatt gttcaaatcc
     7561 atttgaagca aatgaattct cttaaaaccc cgttctgagt aagaagcgta aactattcca
     7621 ggcttttcag accatctgaa atttacctga ttgatatgcg tattccttgg taagttggtt
     7681 tttatttctt tgcctgaact cacctcataa agtacgggaa tcatataaac catggcggtg
     7741 tcaccaggtg aacctcggta ataagaaagg agacggggac gatacagtgt atctacgctc
     7801 caatccaata aatacatttt atccgcggac ctgaggtcac agatgtacgt ctgcaaatat
     7861 tttgaatcgg gggaccaatt aactgaaaat ctttcaggac gctcaccgcc ttcgccggta
     7921 ataatatcac cccacccata ataacttgca tattcataat tctttttccc cgcagtgctt
     7981 aattgtttta cttcaccgtt ttcagttgat ttaataaaca aattgtaatc atttacataa
     8041 gctatccatt taccatccgg agatttggat tctaaaagat ttcgtgggga ttcttctttt
     8101 tcagtcaaag tataattagt cagatcgagc aaatatcttt ttccaatcaa gctaaattca
     8161 agatgttggt tatctatata gttaatatta gtaatgggca aatctccggc tgagatttcc
     8221 ttattcaatg aatctgtcag tatttgagcc agtttttcct ggtcaaacaa aggttcgatg
     8281 gtcattgcac tgaattcaat tttgttgaag aatttgtaat ttttttcctg agttgtatac
     8341 caaacaccgg cggagtcaga aaaccaggac ggcataaccc gtaaattgaa aacgcgttta
     8401 tcattcaggt tctgccaaag ataacttacc gctcggttat aatcttcaat agtaatagta
     8461 gcctgggaaa acactttggt aataaagaac acaaaaaaga caagaataaa atttttcatg
     8521 tggatgtcag tttaatctca aaattctttc ttcaattatt taaggaaata ctcttttcat
     8581 taatggtaaa aatcgtcctt taggataact ctctctttga ccaaacgttt taaaaccctg
     8641 gctttgataa aacttttcgg aattaggatc ggattccaca ataacttcag atttttccat
     8701 taccacaatc tgaagagttt cctgcaataa tttcttccca taaccttttc cgatgtattt
     8761 aggtgcaatc cataaatgaa ttacttcata agcatcaggc tttttcacaa ttgcacaaaa
     8821 tccgatgatc ctatccgtgc tgattttata aacatggttt tcctccagaa atttgcgggt
     8881 tatgcggagt gtttctaccc agttttgcaa ccaatcaagc gggtaacccc agtgcatttt
     8941 tgactctaca cttatttgat taagaatggg caaatcgtag ggcatggccc tttcaactct
     9001 ggcgtcgtta ctcatataaa gaattgctaa gtgaatttaa gcaaatatct ttataagtaa
     9061 atacctgaga agtttagggg ggaaggagag caggtcttac gacctaccct cccctacaat
     9121 cccccaaacc aaaaagagga tcttacggcg ttttgccaat tttttttatt ttgaaaatta
     9181 acatgaccag gtatatcatg gtgccccaaa tacttataat gatcaggttt ggaaaaacca
     9241 ataaccaaat tggtgcctta tcacgggagg cccaaagcga tctcccacca aacgagtcat
     9301 cactttcaga gacaatagtc ccccactgtg cctccttctc cattttcagg tttcccaggt
     9361 tgtcatcatc ttcaacaaaa gccatgattg ttaatatacc atcgggacca ccgggaatat
     9421 cttcaggaaa acttgcggta aaaacaccct catcgtctgt ctcataggta tcttctccga
     9481 ttgataatgt cccaaatgtt ctttttacct gcagttttac aaacacttcc tgagcaggtt
     9541 caattgttcc atcttctaaa tatttgttga tttgcacttt tacttctttc tcctcgcctt
     9601 caagcaattc aatggttatc ttcgccttcg taatttcaat attatcatca ttgtcttcaa
     9661 agtttggatc atcggtaacg gaggctatga aggtaaaaaa agatgttgta tcccaaagtg
     9721 aattaaattc tttacctggc tggagattag ctttgccatc tgaatccgtt gctaatgaac
     9781 caagtaaatt caattcatcc atttcagaat gatagaattt tactttcaca ttttcaacag
     9841 gctgataccg tcggtctacc ctggttctga ttaatgcggt tagagttgga gggccattat
     9901 ttaattgatg gaaagaaaga tcaacgcgta cagcagcttt ttcctggggc attgctatag
     9961 aagcgcttaa aaatatcaac acaagaatta ccaaatattt tctaattctg atcatgattc
    10021 ttagtgtttc gttcatgaat tgtttccgct atcggaacga tcggaaaata ccttacaaag
    10081 aaagttacaa ttagcaatgc tcccgcaagg gagccaatgg ttatactcca ttcttcccag
    10141 gttggggaat aatgcaaata attcccgggc acatcgtaca tgggcagaaa aggatgaagc
    10201 agagtaggag ttacaatcag gaatcgtttg aaccaagccc ctgcaacaac taatattccg
    10261 ataataaaca taggcactgg tcttcttccc ttcggaaata ataacactat caccgggata
    10321 accattccca gaagaattac aggccaaaat atggcagcgt aatctccggt gaataatccg
    10381 gtaagatgtt ctacttccgg tttcttcatc ttaaatgcag gaacaagata ttcattgata
    10441 ttgaaataca gataaagcaa actgagcaat acgagtaatt tacccatatg atcaaaatgc
    10501 ttttcagtga tgtaatcttc aagtttgtat gttcggcgaa gagcatacat cgctataaca
    10561 acaccgcctg ccccaacaag aaacgctccg gaaacaaagt aggctccaaa gttagtggaa
    10621 tcccatcccg gcctataggt tgtggcaaag agccaggagg tgactgtatg tatagccagt
    10681 gcaaccggaa taataataat cgaaagtatc ttgatggctt tttctaccag ttttttttgg
    10741 tctgcacttc ccacccagtt gggattcatc catttctgaa tttttctgat aaatcccgga
    10801 atgggcggat tatatttctg cataatcttc aaatccggaa tcaggggaaa ataaagcaac
    10861 agcaagctta tgaacaaata ggtggtaatc acgattacat cccaaattat aggagattga
    10921 agccgtccat aagcgaataa atgcaaaagc cggtctggcc tgcccatatc aattacaata
    10981 attagtgaag cgaaaacaat ggcagcaact gcaattattt ctgcgattcg ggttaagggc
    11041 gtactccagt ttgcattagt taatcgcaac accgcggtta taagtgaacc cactaatgaa
    11101 atggctacga agaatacaaa atttgaaatg tagatacccc aggaagcata atctctcatg
    11161 gcggttattc ccaagccaaa ccgcaattgc tttgtataag cgtaaagtcc aaaagcggct
    11221 attgccagga gaatataaac ccagatttta ccggccttgc caaattttac cggcattaaa
    11281 tcccgggcta ttttttccga attcatctcc attgctagtt ctttttctct tcttctaatc
    11341 cttcctcaaa tgggaataac ctgttagcag gtggcagata ataaaccctg ggttttgtac
    11401 ccaaatcttc cattaacctg tatcctgcct tgtcttttat tagttgacta aaccgaaaag
    11461 tttctgctcc gttagtaacg gtatcttcat tcaaatcacc aaaaaagaaa acaccattag
    11521 gacaagcaga cacacaatgc ggcaatttcc cttcccgagc catatccggg cagaaatcac
    11581 atttattaac agtacctatt tttggcggaa cgctggtttc ggaactgtat tctcgttgca
    11641 aaacttccag gggtaatacc ggctcatccc aattaaaaac ccgggtggaa taaggacatg
    11701 ctgccataca aaacctgcaa ccaatacatc gttcattatc aattagaacc aggccgtcct
    11761 cgcgtttgaa tgttgcatca accggacaaa cttttacaca tggaggctca tcacaatgca
    11821 tgcaggttgt aggctgccaa tagggggcgg tatgtggcgc gtcctgcatt ggataaattt
    11881 ttatccaatt ctggccttca gtaacaaaat gcattttatt acaggccgcc tgacactttt
    11941 taagattttt gcaccttgac aaatcgatta ccatgacaaa cttcttaccc ggaattcctt
    12001 tccttgcatc tgaattatgg acatgttgtg tttcaggcac cggctttaaa taggctctgt
    12061 caatttcaat aacatggccg tcatcagaga gtagttttac aatttcgcca ctttccttta
    12121 cattttcttc aagcaatggt gagaagggat tctctgccaa agaaacagcg ccaacacccg
    12181 acaataagct gcctttcaaa aattctctcc tggtttgatt atgtttctct tccatttttt
    12241 ctccggttat gtattatgtt tccaaatcca ggtttgcatt tccttaaggt ttaccttgtg
    12301 cgttttcttc ggaagtctgt caagtggtac ttccactaat ttgccgtctt tggttaacat
    12361 ttttacaacc tcaggttttc ttcgaattga aaaaatccat gctatggcaa ttcctgctgc
    12421 ggaacccaga atccatttga gatattttct ccgtaaattg cttctctttt tatctaaaaa
    12481 ctccataatc tggtaatatt gaaaccaatc aaaaattcac ctttagtaaa atcatattca
    12541 ttaaaagtat tattctcctg gggaagaata ccctggtaat tgctcacaaa tacctgaaat
    12601 gcatgtgagc ctgtgctgaa ttcaagaccg atggttatgt taggttttaa atcaaaattg
    12661 ggatcatcat ggttggtaat ctgttgcaca tagtttataa gaattgctcc ctgagaactg
    12721 aatctaaacc ggccaccggc acttattgca attatatcat tgctataaag ggaatctatt
    12781 gcattgtaat gactgaaact gggagcaacc tgaagggaga gttttgaact taatcgtgag
    12841 gcaataatta gttcatggtg gtaagaaaac cggtgaaccg gtttgctaaa caggctttta
    12901 tctctgctat ctctggcaat gtttgtgtaa taggttaaac ttacaggaat actccagtcg
    12961 cgccgttgtt ttaaaattgc atacttcaga ctgagatcca ggtatttttt gaattttcca
    13021 aatccaaatc caaccgccag attatttatg ggtgtatagg taaatcccat tctaatattt
    13081 gcaggtgcat aaatgccaaa aagatcggtg attccttctt cgacagttcc aaaccggtgt
    13141 tgaatatcaa attcaagagt tttatggttg ggaaccatta cggactgcaa atccatgagt
    13201 aaagcgcttt caaatgttgg cctgacaggt ctattatcag cgtctgttcc ttcttcttct
    13261 tgtgcaaaca aagaatttga tattaatatt ggcaataaga tgagtaattt tactcctcct
    13321 gataaattct tattggtttg gtttttcatg attttttaag atttaattgt ctaaagcacc
    13381 ttgttcaatc catttaagta ttatggcccg gtcctgatca gttgcatagg ttttcatttt
    13441 cccaatgttt atttgctgat agatcttgct attagcagga tcagccaaat tcacataacc
    13501 aaagaaggta aggctattgt atgcattgcc gatagtcagg tttggttgct ggcctccggc
    13561 cgaatgacaa ccaggaagtg cgcatgattt tgtaaatatg ggttgaacat caatcgaaaa
    13621 gctgacattc tccggaactt ccacttcctg tggaataatc tcatcataag tacacgaata
    13681 aatgatcgta cccaggaaaa atattatggc cataatgctt tttattccct gtttcatttt
    13741 aattttttgg tttgaaggtt aattcaatat tcatttctcc aatcttgttt aaatcatcat
    13801 tgtcagataa atacacatga aaagtatatg gagaattgtc cgggttaaaa acaatgtcat
    13861 caaggttgcc tgtgtttaac tgcctgatca gcaatactac gtatcctcca ttagaagctt
    13921 tttcatcaat caatgccaat tgcacatttg aaagtgcact gatgtcagaa ttactgccgg
    13981 ttggattttg aattaaataa cctggtattc cggaatacgc ttttagcctg gcataaagat
    14041 tcactctctc ctcttcggtt aatcctgaat aataggatga cccgtcatgg ttcccggaac
    14101 tgacaaaagc ttgaaaggca gtaaaagtct gtctcataaa cctggctcga tcatttaaag
    14161 ctttggcctc ccctcctacg gcattaaccc catgacagga tgagcaggta gtttgaaata
    14221 ccatgtcgcc aagttctgca tcaccggtaa gtggcttggt atttaacagg taataccccg
    14281 gatctaaaaa tgttagtccc cccggggaac gatcaagttg ctggttatct attccatccc
    14341 agtcaagcat aggaccggag cggttatctt cagggtctac tgcatttctg ataaatatct
    14401 tatctcccgc gtcaaactga ttccctgttc cactattgaa taaatccagg gcatacccaa
    14461 gtggttctga caaagcaagt gaccacttcc aaacgtcaga ttcattcgat gacacgggaa
    14521 atacaaaaat tatgttatca tcattctgct gggacgtcca ccctgttgtt gaaccagact
    14581 tatcaggatc cgagggtcca ttcagaaacc aggatccatt tgacgcattg taagtttcgt
    14641 cctgccaact cgccaatatg tacaggtaac tgtcatcata tccggctttc aattccaaaa
    14701 taggatcaat cccttcattg aaacttgagg ttccgttaat agtcccactc atattaaata
    14761 atccatcatc ggttggaact tcaccgctgg ttattgttgt ggctgtaacc tcataatagt
    14821 ttaaggtgtt ccaaaaggga gctgtaatgc tattaggaat tgttgttgta taaaccgctt
    14881 ccagcgaagt tgttgctttt ggaacaaatg gcttgggagg tggttcgttt tcaaagtaag
    14941 tgcatcccgc cagaagagcc ggaaatatca taaatatttt tagtctgctt ttcatcggct
    15001 tggggtaggt agaaaaaggt taagggagtg ttttcactcc ctggagttac tacttattct
    15061 ttctcagttg aatattaaca tatactttga ttggttcatc aacaatattt gatgagaaaa
    15121 tattgaaatc tgccttcgcg ttcattatca ttttaccttc aaagcttgca taaaatctgg
    15181 ttgcatcttc agttccggtt cggtatttaa atacaatttg ggctgctttg gttaccatcg
    15241 tattcgggtt attggtaatg ccataagggc ttgaaggagt tgttgacatt accgcagaga
    15301 aggaaaggtt tcctttggca ataaaaccat ctccatacgg ctccacgctt gtgctggtaa
    15361 aactggcata tcgctgaggg tctgtaacgg tagctgggaa tgttgcatca gcgaatatcc
    15421 caaaggtagt tgcaatacaa tttgtttctg taaatacagt tgattcggtt aatgcgttaa
    15481 actcaccatc ccaaagtgga tcacgtccgc ctcttgtacg ggtattaaca ctcattaaat
    15541 caacttcagc catcaaagag ctggtggaaa gactggctgg gtcaagattc aatatcaatt
    15601 tggttactct tccgaatgat ccggtaaatt ctgaattttc ttccttaaaa tcgaaagcga
    15661 aatctaccgt tgtatgattt gggtcaaatt cccattctgt ggcgtttccg gctcctcctt
    15721 ctcttccatc ataatttatg tccattgcac tggccattgt ttgacctaca gatacaagtg
    15781 ttatgttttc ggtgacatcc acaccaccaa ttaccacttc aatgtcatca cctgcattga
    15841 agttaacacc ttccaacctt gctcctaaat tctggttatc agtgtaataa ttagcattaa
    15901 ggtaatatgt tccggcctca agattatcaa aaatataatt cccggaagca tccgctatag
    15961 aagtaaaatt atattccagg gcatttgcag ttttagcaag taaaacaact gaacctcctg
    16021 ccgcagtgtt tccgctaatt ccaggataag ttactgtgcc tgatattgat aataatggag
    16081 caggatcatc atcactacat ctaacagtta gtaataggag tagggtactc agaaacgata
    16141 cccataatat tctgtgcgtt ttcatcttct ttttgattta ggtataataa ttatttttgt
    16201 aaggtatttt tactatcaag gaaatactat gacctcgatc aatagtcacc atgactttta
    16261 tcatccaatc caattatttg aaggaaaact gaataattga ctctctgaac ttttcggtaa
    16321 gctcctgata ttctgattct tctgaattaa ttgggtggat tcccgtttga gcgagaaagt
    16381 tccagctact attttcccac agatatattt ccatggagta agaaggttcc ttttctggaa
    16441 ataaatggat cttaagaaga cgcgaatctg tttccggaat aattgcctca ctaaatggtt
    16501 gcattttgtg cacaaatgtg tgtggaggca ttaagctgtc tatggctaaa tcataattca
    16561 gttcaaattc ctgataaaaa ggctttgtgt tacctgacga atttgtgatg gcatattttc
    16621 taagaggaaa cgattgctct aattcctgcc taatgatatt tgccgccggg tccggattaa
    16681 tgatttcaat ccgatagtaa aaaattactt ccggtgcgat ggatgaagaa gtaatgcagg
    16741 atgcggcaat taccaataag atagcatacg gccgcatcct tcttaattca atttaataaa
    16801 ctttactctg caaaagttct taagtaattc acaattaacc acctgtcctc atcactgccg
    16861 attttcttcg caaattcagg catatcgccc cggccagtag tggttttgta gaataacgcg
    16921 ccatcagatt gcttttggaa atcctctgca gaaaaatctc ccgttggcgt ttcaagttcg
    16981 ccggcctttg ttccatcacc taagccttcc ttaccgtgac atgatcggca atgcttgtcg
    17041 tataattgtt ttcctatcgc gaggttttct ttgtcccctt cagtcggatt tttcatactt
    17101 tcatatttcg cgggaacttc ccacttttcc tgaaatacgg tgaaagaaaa catgaaatag
    17161 acagccacta taaatagcag gtatttcaat ttaaacttct tcattttatt agaattttgg
    17221 tttaggaata ttttcaacaa ttattgaaag gtatccggtt tgtataatga taaaaatgat
    17281 aattatcatc taatccggtg attaataaaa cttctattct tgagatgaaa atgagaaggt
    17341 gtacttctcc agaaattcag actgaaatgt gaatttgcga ttatgcggct ttcttggcag
    17401 cttccaggct aattccctgt tgcttcgcta acttaataac tcccacttta tccagaattt
    17461 tgataaccag gtaagtggga tcaatttcat gccatctgaa accaaagttg gcccgggaac
    17521 cattcttgtg atgattgtta tggtaactct cacccatcat gagaaaatcc acaggaagga
    17581 aattgcgtga agtgtctcca acttcaaagt ttcggtaacc gtatttatga gcgaaccaat
    17641 ttatgattgc tccatgaatt ggactcatca aaaagtgaat aggaagcaga agccacatcc
    17701 accatgcagt tgcgaaaaag aaatagaaga ttacgtaagc cgttccccat aatatcctgg
    17761 agggccagga tttggcaatt ctgtcaaatg catgccagtc aggaattccg tcggtaaatc
    17821 taggatcggg tttcacttgt ttgtttgaga aaccggcata aatagttttg gtcttccaca
    17881 tcattttgaa aatggactca tcgtattttg gagaatgcgg atcattaggt gtatccgcaa
    17941 atgcatggtg catccggtgc ataattccgt agccataggg actcagataa ttggagccct
    18001 gaaatatcca tgttaaaata taaaaaattt tctccttgac gggactcata gtaaatgcct
    18061 tatgtgccgc gtacctatgc aggaaaaagg tctggaaaaa tagtgaaagg taccaatgtg
    18121 ctataaagaa aattaagatt tccataagtt aattaattgg gattaagcgg tttacaaaca
    18181 aacagtaagt ggattatatt gggagagaca attttgatta tgaattgtga cagatccagt
    18241 tacttttttg cgggtaaatc agccttgagg taattaatta agtccgtaaa ggttccactt
    18301 tggcaggatt tccgaaagat ttcagcaagg aagttactct tgtaattgat ttgaaagctc
    18361 atctttgaat tcccaagctt ctttttagcc cggctcaaaa tttgtctgca atggtctgcc
    18421 ttctttccaa caattttcgt cagaacttca taatcaaaat tcaaaacctc ccgaagcagc
    18481 acaacagctc gttccaaagg ttctaatttg tgattaataa ttgacaaagc tttgcctata
    18541 tctatgtcaa ggtcaaaatg agaaagatcc ggttcaatat gtcctttgtg tggttcgggt
    18601 accgcctcta aagactccgt cttcctgaaa ctaaatcttc taagtttgtt tatactaata
    18661 ttcctcacca ttgaggtaag ataggccttt gcgttctgca ctttattgtc atcaaccgac
    18721 agccaccttt caaatgcgtc atgcacagcg tcttctgcat ctgctttgca ccttaatatt
    18781 tgcaatgcaa tggaatgcaa taacgtctgg taagttaaaa agacctgggt ttgattcata
    18841 tcttatttac actacaaatc tacaactaca aaactataat catttagagt taattgaaag
    18901 gaatagcggt tcattgttgc aaacatttat gaatctccat ttgcctattg aagttcttta
    18961 aacaagcata tcccctattt tttctctttt tgaccctttt aagggtatct gtataccatt
    19021 tgtaatccca attttttagc gataaaaccc catgtatact gctgtcattg tatggattga
    19081 aactgtttga ttctatttgt tcaaccatat tcataattaa tcaaagttag aattatcaat
    19141 gaaaaacgcg aaattttcca atcagccaat tctcaaatta atgtcaaaat tgcaggactt
    19201 tatactcatc ctgtcagcaa cgctgggagt attcattgaa agcagttttt ctccgaagtt
    19261 tgtttttggg attttctttg atatcatatt ttggttcaca atttccggac tttatatcac
    19321 atacttttgg aataaaggaa gcaagaattt aagaaagtat ttccttcaca ccatatatat
    19381 acttatcaca ttatttgcag ttctgtttca attaaagaat cccggatctg gtgttgctca
    19441 aattattacg cttttaacac tttttatttc cggatcgatt tttctcaatg tttttaggca
    19501 aaccctattt ctggtattcg ccattctttt gcgatttggt tttagcgaat tctttgctcc
    19561 aatggatcca tatttccatt attcattaat cgtaatcgtt atcatctcaa ttgtatccgg
    19621 attcttctta ataaattttg agaaaacaat tagggatgcc aatgcattat ttatggagtc
    19681 aaaccaaatg ctgatagaat tagatcaaat ttatgcgcca aaatataata acgctggttt
    19741 tcattcacat ccaggatttg aagagaatga agttatgttt gttcctccta ctcttgagat
    19801 aatgccaggt accggacata attcaaatgg gaaatcagtt attttagatt accccaacct
    19861 gaaagttgaa tccgcaaaaa aggtaaaaaa tgattacaaa agtgaacttc aattaccatt
    19921 cctgataaat tgtctcagga aggatcttgc ccgggaagga ataaccctga aaacacaatt
    19981 aataaatgaa ttcaaattcc aagacctcaa aatgcggtgc ggagagatgg tggaccacct
    20041 gcatgatttt tttctatcag agaaagaatt taacagtggc aaaatagtaa ttgataacac
    20101 aacaactttt aatgaattca ttatctcttc agagtttaat tggcaaagag aattctcact
    20161 ggcagggatt ccatctgatt tacgttcagc tgtttatgat ttgggcggca agtgttttta
    20221 taattcggat caaaacgaaa actcacgctt aataatttgc ttgcccaatc ctcaggtaat
    20281 tcccggcagt aaattttcca ttctaaatct acattcatta actgatcttg tttttgtgaa
    20341 tgaaataatt aatgccatgg atagatttct ttgttctctg gatttttcag tagaaaattt
    20401 atccagggaa ttgggaacaa gccgcatgca gttacataga aaagtgaaag gctcatttgg
    20461 aatgagtcct ggcagtttgt ttcaatacct aaggatgaaa aaagccgagt ttcttttgaa
    20521 atccggatat tcccgcattt ccgatattgc ctactcgttg ggttattgtg accaatcaca
    20581 cttctcaaaa gctttccggg cctggtctgg ttatccgccg gttgagtata tgagaaaaaa
    20641 tcaggcctaa ttttgtctac ctgaacattt catagtttgc agtgcaccag gaagactaat
    20701 tactttttga tgctaatagg taagcccagg ctaaatcagt ttcccctctt tcaagagccg
    20761 cttttcccag catgtgcaga tggtattcca ttgattcctt atcgttgata aatcttgtaa
    20821 atacatctga cacctcaact gaatttttta tttgctccag aaattcttta ttcggcaatt
    20881 ctttaacacc cgcaagcctg gcaaggttat taccggatag caccggactt ttcttaattt
    20941 catccggcaa agaatcaaat ccaataccta aatgttgtgt gggtttcggc actctgaata
    21001 tcgaatccgg aattaccctg caataccaat cttcccctaa tctgccgact gcatcaagtt
    21061 tcaccggatt aattttttca ttttcatcca gaatctcctc agtaatatgc attaataaga
    21121 tttcacagat taccagatta cccgctcctc ctttatctcc taattgaatt acctcattga
    21181 cgttgcattc aaaagcaatt ggtgcttcca acaccctggg tggttttacc attgctgatt
    21241 ttacctcggt tagtcccgct tttacgaact catttactcc ctttgcatag ttactgctgg
    21301 caagcgacat ttgttcaaca agattgtaat tggcaatatt aattacaacc tccggcactt
    21361 cccgaatatt ttcgtaagta tgcttggtcg tattatcttt acccctccgg gatggagaaa
    21421 aaaccaggat gggcgggttt gcactgaatg cattaaaaaa actgaaggga cttaaattaa
    21481 catttccctg gttatcaaca ctactggcaa atgcaacagg tcttggagta actgcgccta
    21541 gcaaataaga gtgaaactcg gaaagtgata aatcttttgg agaaaatgat ttcattgtca
    21601 tctattttgc cggcaaaacc ctgtttcgaa cttctccaaa gccaacacgt atgccatttt
    21661 tttcacagta cccgcgtaga attacctcat cgccatcatt aataaacttt cgcacgctgc
    21721 cgtctttcat tcttattggc tttgttcctt gccatgcaag ttcaagcata gaaccatacg
    21781 tatccggtgt tggcccgcta attgttccgg atgcgtacat atcccctatc tggatattgc
    21841 aaccattaac agtatggtga gccaactgtt gattcacgtt ccagtacatg tgtctgaaat
    21901 tagatttgga taccgaaact tcttccgact tgtcaggctt tataaaaact tcgaggttaa
    21961 tgtcaaagtt ctttgatccc tcaaatgcga ggtagggcaa aactttttct tcctgagttg
    22021 gcccgtccac ccgaaatggt tcgagggcgt ccaatgtcac aatccatgga gaaatggcgg
    22081 acgcaaaatt ctttgacaaa aaaggtccaa gcggcacata ttcccaggct tgaatatctc
    22141 tggccgacca gtcattaaag agacaaaaac caaaaatata atcctccgca ttagccaaag
    22201 taatctgatc gcccagagca ttgcttttgc aagcaacaaa tgctatttcc aattcaaagt
    22261 caagccgttg agtcgcaccg taaattggcc ggggatcatc agctgatttg gtttgtcctt
    22321 taggcctata gatatcctgc ccggaaataa ttattgaaga tgcccggcca tggtaggcaa
    22381 ccggtaagtg tttccagttc ggaagcaacg cgttctcagg atccctgaac attgtaccga
    22441 tattaattgc atgctctttg ctggaataga aatctgtata attacgcaca ttgattggca
    22501 tatgcatttg tgcatcagcc atgttaacga aaacttccct aatttcattt tcattattcc
    22561 ttaagatact attttctgaa tccagaagtt cagagacctt catcctgacc tcaccacatt
    22621 tcttgcgtcc cacctctaaa aatgcattta gaatatcgtt ggaaaaaacc gccgtatcta
    22681 ttccaggaat gtcaaggtat ccgcgctttg ctaactctga caaatcaacg atttggtttc
    22741 cgatagcaat gccggccctt ttattccgtg ttcctgtact gaaaattcca aatggtaaat
    22801 tttgaattgg aaaatcagag cctttcggta cttccaccca ggatttcagt ttagcatcat
    22861 tcggatttcg cataggttca atctgtttaa atcaaaaata ggtgaataca gacaaaatga
    22921 aatgaagatg aaccactatc ccttaaattt ctttaatttt gccagcttaa tgtgtaatta
    22981 gaaaagctct gatgtccgtt tctacaaaat atgatccagc aaaagttgaa gacaagtggt
    23041 ataaagtttg gctggaaaat ggttatttct cttcaactcc gaatcccgat aaggaaccct
    23101 attgcatcgt gattccaccc cccaatgtga caggagtgct gcatatgggt cacatgctta
    23161 ataataccat tcaggatatc ctcgtgcgta aagcaagaat ggaaggaaaa gaagcgctct
    23221 gggtaccggg aaccgatcat gcttccattg ccaccgaagc gaaagtagtg gcgatgctta
    23281 aggagcaggg catttccaag aaggatattt caagaaagga atttgttgaa aaagcctggg
    23341 aatggaccca taagtacggt ggaattattc ttgaacaatt gaagaaactt ggtgccagct
    23401 gcgactggag tcgtactcga tttacgatgg aggatgactt atcaaaaatg gtaatcatga
    23461 ctttcataga tctatataat aaagggtata tctaccgtgg ctataggatg gttaattggg
    23521 atcctattgg taaaacagct ttggctgatg acgaagtaaa ccaccgcgat gtcaattcga
    23581 agttatacta tattaaatac aagatcgaaa attctcagaa tgaatttatt accattgcta
    23641 caacaaggcc ggaaactatt ttaggagaca ccgcagtttg tattaacccc gcagacgaaa
    23701 gatataaaaa ccttgctggc aaacgagctc tggttccgct gatcaatcgt tctattccaa
    23761 taattgacga cgattatgta accatggagt ttggaaccgg ttgtttgaaa gtcacccctg
    23821 cgcacgatgt taatgactat gaattgggga aaaagcataa gctggaaacg gttgatattc
    23881 ttaatgatga cggaagcctg aataaatctg cccagctctt tgtaggaaag gacagattcg
    23941 aagcccgtga attgatagtt aatcaacttg aaaaggaagg acacctggtc aaaacagaag
    24001 cttatgtaag cagtattggt ttttctgaac gcactgatgc gataatcgaa ccgcggcttt
    24061 cgatgcaatg gtttgtgaga atgaatgacc tggtaaagcc ggctcttgag aatgtaatga
    24121 atgatgaaat caaattgtat ccgcccaaat ttaagaactt gtatcgtcat tggatggaga
    24181 atgttcggga ctggtgcatt agccgtcagt tgtggtgggg tcatcaaatt ccagcatact
    24241 attataccga taatgagttt gtagtagctg aaactccggg taaagccctt gaattagcgc
    24301 aagaaaaaac cggaaatacc aaactgaaac catccgatct aaaacaggat ccggatgtat
    24361 tggatacttg gttttcctca gggctttggc caatttcggt ctttaatggt ctgcttgatc
    24421 ctcaaaataa tgacattaag tattactacc ctaccaatga cttggttacg gcgcctgaaa
    24481 tattgttttt ctgggtcgcc agaatgataa tttttggcta tgaatacaaa ggagaacctc
    24541 cattccgaaa tgtgtatcta accggaattg taagagacaa actgggcaga aaaatgtcca
    24601 aatcgttggg taattctcct gatcctattg aattaataaa aaaatattca gctgacgggg
    24661 tacgaacagg aatgcttttc agctcacctg ccggcaatga tttgcttttt gatataaagc
    24721 tctgtgagca gggaagaaat tttgcgaaca aaatctggaa tgcatttcgt cttgtttcag
    24781 gctggaatgt agataacgac ggtttttcga cgcctaacca gattgcaatc gattggatga
    24841 actcaaggtt taatcaatct cttgaagaat tggaaagtca tttccgaaaa ttcagaattt
    24901 ctgatgcgct attgacagtt tataaactgg tctgggatga ttttgcttcc tggtatcttg
    24961 aaatgattaa gcctgaatac ggcaagccta tcgattctca aaccaaacag gaaacaatcc
    25021 gaattcttga aaatattttg aaaatcctgc acccctacat gccttttatt accgaagaaa
    25081 tttgggatca actgggtgaa agggacaata aaagccgcat tatcatttca ccatggcccg
    25141 cgggcggcaa gatagatcag caaattatca ataaagctaa tcattcattt gacctcatta
    25201 cagccatccg gaaggcaagg aacagcagag gaatttccct aaaagaatcg ttcactttac
    25261 aattgctaaa tggagattcg caggttggtg agtttgaaat tttcattaag aagctagcca
    25321 atgtttcatc ttttgaaaaa atccctaaat ctgattcgga tggaatttcc ctggtggtaa
    25381 acaatgttga ttattttatt ccactaacag gcaaggtaga caaggagtct gagaaaaaga
    25441 cattgctggt agaattaaaa tatgcgaaag gattcctgga atcagtcaat aaaaaacttt
    25501 ccaatgaaag gtttgtgaat aacgcacctg aatcagtggt tgaggttgaa aagaaaaaga
    25561 aggcagatac tgaatcaaaa attaggtctt tggaagagag tttaagccgg ttgaattaga
    25621 acttgatctt taaagtccca accggagatt ccgtgtaaat actggccggg gcttagttgt
    25681 ttcaattaat gggcgggaga aaagaaagtt ttctgcgcaa atccgcgaaa tctgcgggag
    25741 aatattttac ttaatcccgc tgataccgca gatcatcgca gggaaaaaat ggttcgggga
    25801 atcttctgcg taaatccgcg aaatctgcgg gagatttttt ttagtcccgc tgataacgcc
    25861 aatcgtagca gaggccgatg ggtttggcag gccggattta actatatttt cagtgttcat
    25921 ccaatggcag agaaaggttt ctgaggagtt caatggtatt tttctcgatt tgtttgaagt
    25981 agttcatgtt ggtattcctg attttgcaaa attctctttt acctgttagc gggtctacaa
    26041 aagaaatata ggtagtgaaa aatccaccgg aagcatttat tttcatttca agaggcatcc
    26101 acagccattc caggctgtca tgtttcatta tgataccgac cggaatttcc attattttat
    26161 tctccaatga gtcaagcgga aagattaagt tatattccct gagcatcaat aaatgctggc
    26221 ctgctatatc tttttcatca gcaataagat tgcattgggc gaatgaaagg gttgggataa
    26281 gtgtgaggat gagaattttc atgaaggaat tatttttcaa gtggcgggtg ctgaaattgc
    26341 tgccagaatt ctttttccgt ccagtcgggg atttcttcgt tgcgccattt cagggcatcg
    26401 aacgggtaac cttcaggaaa gtctacataa atcagatatc ctttaaagtc aaaagcaaat
    26461 ttactcttat agtaagagta aaaggttttt cgttccgcat aatctcttcc taaaagcatc
    26521 tggtattttt tgtgttcgac atctacgatc acggcagtgg ttttctgcct atcccagaag
    26581 cgggtaccta aaatatattt gccatctggc gagatagcta taatatcagt attggctgcg
    26641 accttcactg agtcttccaa attaccccaa ggtgctaaat agatatcttt attactataa
    26701 ccaccataac catccttccg gccgatggtg aaaaacaatt gatccccaat taaataacta
    26761 tctgaacaat gggtacaggg aatgtattgt tttccgatct tgccctgttc aatctcacta
    26821 agatcatata ccatgatgcg gttgtcttgt gcagggttat aataatccga taaagtgttc
    26881 aatgtattaa caaatagata cttgccatcg ctgctgaaag aggagtggat ttttttatca
    26941 gcgttgtaga tgctgtcgag gaaataatct tttccattgt gctgaagcca gaggttttga
    27001 tagtagatgg tgaaatcatc attactgaac attactttgt agaaccaaga agatcttcca
    27061 aaatcaataa gtcttagaaa accagtttca atatttgaag tttcaaaggg cggggtaaat
    27121 ctcagattaa atatttcaat tttttccact ttttcgttaa taataatact cttatagcta
    27181 ttgactttac gccctgctct actcacaaag attggactac cttcctgaaa tccgaggaac
    27241 ccatattg
//
LOCUS       DASWHX010000042        19574 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_2990795, whole genome shotgun sequence.
ACCESSION   DASWHX010000042 DASWHX010000000
VERSION     DASWHX010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 19574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19574
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2990795"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..299
                     /gene="crcB"
                     /locus_tag="VGA21_02420"
     CDS             <1..299
                     /gene="crcB"
                     /locus_tag="VGA21_02420"
                     /inference="COORDINATES: protein motif:HMM:TIGR00494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEX9649391.1"
                     /translation="FTRFLSAFPYGTLAVNLIGCFLIGLFFGFVEKTDVNPDLRIFLM
                     TGILGGFTTFSAFSNETVELLRQGSFGYASIYVLSSLVLGILLTFAGIVLSRQL"
     gene            504..845
                     /locus_tag="VGA21_02425"
     CDS             504..845
                     /locus_tag="VGA21_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020530725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX9649392.1"
                     /translation="MVTYSLGEFEEVVLLTVAILQKNAYGVSIKLDIQKRLNRKVSVG
                     ALQSALRRMERKGYIKSHFGEATPERGGKRKRYFEITLNGKKAVSHAMEDRISLWKAS
                     PELLTELGRLI"
     gene            842..3442
                     /locus_tag="VGA21_02430"
     CDS             842..3442
                     /locus_tag="VGA21_02430"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9649393.1"
                     /translation="MIRRNWIYSIIRPFIRPEFLEDIEGDLKELYESTNATRQGLIFH
                     LKFSFEVFKLLRLELINIPFNVVPKLKLYMIPHYFKVAFRSLLKNKLTSFVSLAGLSL
                     GLACVMLAYLYVSHHTGFDDFHTRKDRIFRVVNGDMDSGEGWVKIAAPLLPQLKETIP
                     EIESFTRFEPISRGEKVLIESENKKFNEPYFVMVDPAFFEIFDFPLLIGDKSLVLNEP
                     GNVVITKRIREKLFGEANPIGKVIKADETHEFIVSGVIENPLLQSHLRYDFLISFENL
                     KNIYGENSVNSWGAYNYFGYILLNEKADPFITAEKIRDFSVTTDRSTFNFDQVNLQSL
                     SAIHFQHNRGNLLPGINPVYLWIFASISLIVLIIALANYVNLSIALSIRRAREYGVRK
                     ALGAARENLIIQFFSETLIAMFISLMLAALFIYAVLPPLNAMLGTSMTLKFDKPEFWF
                     ALTGIVTLATILASSYLAIIVSAFNPVTILKGNIKVKKGSGLQNILLGIQFLLSIVLL
                     INTFIITDQLNYIKTRNIGMAPDGVVNIPLYNKLDSVKLNTLKNELLKVSGVTGVSAS
                     TFVPGSASWHQTVSWEGQTPEEQDNAQMYLIATDKDFMRTLGLELVYGNLEDIENSST
                     PQIKYIINESGMKFLGWEDPGERKLSPFGPGNYGEIAGVVKDFNFRSLHSGMDPLLLA
                     VSDRFFHEQLSIKLQIPNLTSSLKDIEKTFVSVSGGLPFEYFFLNDKIEQLYQTENET
                     GKLIFYSTLFALLLATMGLFGIISFMIEDKTKEIAIRKILGISFFQMIFMFSKRYLLV
                     LSVAIVFAWPLSWFVMNNWLNNFAYRTGLDFEPFVIGGAVAFLMIIGVVGIKMFTIKN
                     LDPAKALKQE"
     gene            3790..4254
                     /locus_tag="VGA21_02435"
     CDS             3790..4254
                     /locus_tag="VGA21_02435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649394.1"
                     /translation="MKRISILILVTISAGIFQGCIFDKDGCTSACASNFDSSADNDDG
                     SCTGCTDPTATNYCPGADFDNGTCILPCEANQSGKVYFINNSNSNSTYDVIWDGVKIF
                     TVAPGQQSSTITVTANIQHTLVFRYTNTSNNACTPSTPVIPQCETWWFNCTG"
     gene            4697..4771
                     /locus_tag="VGA21_02440"
     tRNA            4697..4771
                     /locus_tag="VGA21_02440"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4732..4734,aa:Val,seq:tac)
     gene            complement(4785..5129)
                     /locus_tag="VGA21_02445"
     CDS             complement(4785..5129)
                     /locus_tag="VGA21_02445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649395.1"
                     /translation="MQGFLRKSAGEKSFSTKICADLREKIFLIPLISQITTERIYSNQ
                     RRSAEEKFFNPADIVDHRTENLFRDFCANPRNLREKRVFLRKSAPTAEEEFLIPLVSR
                     ITAEKWSSEASM"
     gene            complement(5218..5739)
                     /locus_tag="VGA21_02450"
     CDS             complement(5218..5739)
                     /locus_tag="VGA21_02450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649396.1"
                     /translation="MRTYYSLTNSLIRIIIILIPFFVCSCPAIDPSYTQAYYNFITLE
                     GDLFETNQNYLIENLYGINDNGYRRKFGHEGMFNNVNYFKINAWPASPLYIEYGNGDI
                     DTLTVTYGPSGVNPTSEFGKLSFVKWELNGVLIADWDFKKNPELRDSILKENKSGISQ
                     YRTTFPVPVIITK"
     gene            complement(5878..6225)
                     /locus_tag="VGA21_02455"
     CDS             complement(5878..6225)
                     /locus_tag="VGA21_02455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649397.1"
                     /translation="MKKIVIISTVFFWLISCPGESSETNETARWKKWNPLSFEPFLDT
                     NDNWGALWNPDATNLMSYATFDCARDLTFGQIAVMLDNIPNFATTQSGYTINGPQPFA
                     PISPQPSVLTSVL"
     gene            6530..10546
                     /locus_tag="VGA21_02460"
     CDS             6530..10546
                     /locus_tag="VGA21_02460"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9649398.1"
                     /translation="MKRILLFTLILISNLALGQEWLTLYQQSQTDYENDNLEAALKSA
                     LKSLSIYEKENERNNSNYCSILRHLSIISYSAGEINSAIRYGKDEIEILRGLDEVDKN
                     TLAGAINNLALFHQTLGEAHEAESLLEEVVEIYSVIENSGNELYIARGMLAISQFGQQ
                     KMDEAGDNFRVSIGFFANADDLPFDYLTILLNYGIYLVTKEEFSTALTHLSDAKNYYD
                     QFKMDATPEYVLLLRNLGRAKVNTGDAESATAELERALEISRNLYGQNDPETISIKNE
                     LALHYQRLGKTELADALSSDTDITNLDEESKLDYAISQSNLGAIRQSDGDLIKAEELY
                     LEAIEIFSIAEKEVNSYLNALQNLGRLYQTKGEFARAGDQFQKAREIATRTYGESSHF
                     YADALVNIADLYRITNNHTESGQLYLSALNVFESDIDSPQKEIAKAHEGLALHYLSMG
                     NFNKSQENFLSAINIYSNTGDKNKLLTTKSNYAVLKQAMGEYKTAETLLREIYAEVTA
                     TRKGTLEESAAAENLGTILIEAAKYAEAEEYFKIVLRIREGYGKSTPEYAKGIINLAR
                     LKVREGKYTDAEALYRNALEIYSGSLGNNDLRVANLENNMALMYQYMGNMEAAESLLE
                     RSSEKIKNQLGIDHPEYATALENRASLLQILGKREEAMPLLQQALQIDEKTLGRSHPN
                     YAFTLHNLASIYLDEKNYMKAEELFLQSVEIEKSVLGEDHPIYAGTLNNLGVLYEKQE
                     NYVKAENYFNLALSIREKVLGKDHPDYAYSQYGLAVLYLKMGKLAEARTLYETAIASY
                     LDQVQNFFPSLSEKEKSDFYTKIKPVIENFKEFAIEYAVNEDGNMSSKLIGEIYNVQL
                     ATKALLLNASNKVRNRIASSGDLKLIESFNKWIEIKENLARYYQYNLEQLDAEQVDIT
                     KLEEVANQLEKEISLQSQVFASEFDKQPVSWEQIKNKLTDNEAAIEILRVQRNTTTDT
                     VMYVGLVIKKSSDFPKMVIIKYGESLESMMYKFYKNSVTFRAEDPYSYNAFWKDLDTE
                     LEGISRVYVSADGVYNKVNLNTLRNSGSGEFILNQYEIILLSNTKELLTDLSSEAFRT
                     ATLLGYAQYQSVQPENPQPQGVFQALGSGGWLKEGIPALEGTKKEVESISGLLQSHGW
                     EFKTLETTNASESAVKNLKGQGLIHIATHGFFLEDLKIDESGDGFGVHIKNYELNPLF
                     RSGLLLAGSGETIFGTANQSQQEDGILTAYEVMNLSLENTDLVVLSACETGLGEIRNG
                     EGVYGLQRALIVAGAKGLIMSLWKVNDETTQKLMTYFYQNWLSGKTKLEAFKAAQLSL
                     KNEFNDPYYWGAFVMIGR"
     gene            10550..12616
                     /locus_tag="VGA21_02465"
     CDS             10550..12616
                     /locus_tag="VGA21_02465"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX9649399.1"
                     /translation="MYCRKIIFATSVMLFASLNAQFTLGQSAMQIVAGLDTRDSTVID
                     FAPSISADGKTMIFQSNRGINRSRYFLFQAKLDDSGRWGDVTSLDNINNFGDSLDLIG
                     GPSISFDGNTLFFFAFFAGGFGQEDIYYSVRDGETWSAPINIGGVINSSGYEGFPSIS
                     ADGKTLYYVSQNYNGPTDRDIERLNQFCTVLMKSERDESGNWGPPKKLPFPINLDCEK
                     APRIMADNKTLVFASNRPGAVGGIGDYDLYQTRLNILGEWGPPIPLNYVNTIQSEQFC
                     SISASGELMYYVQAGRDIFSVPIPQEFRQFVNTIVQGYITDMDDKSGLAAAIIVTDAL
                     TSDAIMTMSNNPSDGRYTLVLAAGKNYNIEVRKQGYSSYTTSYDLSNVTTYREISNDI
                     QLFSEAKLAVSVSDEELLETITASVTISESGGGGVLQMQSPKTGRLELNLPLGKKYHV
                     EVQKDNFEDNSFELDLTGLVMYRNYEEDVPLKPIKVEVAINVADLTNNSKVKSRVLIR
                     NKNRDETIEVEGNEMVSLRAGDRYQLEVTSDQGYAFNSTEIDLTNAASNPAIAAQSVN
                     VELQKLEANASLTLKEITFESNSFNLSEISFTELQRVVKLMRENPTLKVEIAAHTDDI
                     GSDAYNRLLSQRRAQSVVNFLIGNDISSQRFVSKGYGESQPLIPNDSEEGRARNRRVE
                     LIILAI"
     gene            12619..14790
                     /locus_tag="VGA21_02470"
     CDS             12619..14790
                     /locus_tag="VGA21_02470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649400.1"
                     /translation="MKKIIVISLLVLAGFSLSPAQRIKYKDLVPVLDSLKEDEAIPML
                     IELLQTERDNPNANFRLGLLYAKKYKATDPLKEFEKSIEYANRTKLQMIKSKAIIDNR
                     EVNRNSGWYAPLATEVKGNKVEFIIIQQQIDNEYNLADAFTNNIPPAYASFTKAVFNY
                     DKAKKTYADIAQKYASIKELCLLYNQDIETRMRQLQFQYDSTYYYFNQYNSLKAGTII
                     AYDQKLVVKPINVYRKDGLATQTDFFTQKIDIWNYRKWAEEVINEVNSSVVSLRENLQ
                     SNYEKISKSVEVMESGTDVEQFATIDKQLLFTLGRMDYRNALVPMLQYNQFLQEYLSR
                     KRELSGLESDTAIVLQTKLQLYTLLLYENIKADSLLADLVEKSQDDAKLKRHEEFLIQ
                     NFGSPEGFKKMALDEKQKVEAEYANFVTLIKQAVTKVSMAEEVSGNTITHQRMNLSLL
                     ASPNPVDSLVDNKPVTAHTELNPDGSRYLAGVIRRNTKTRHVEVFVCKVDSRSRVSWM
                     KFFDLQMDNEKSLSNHIPTAIRATQKGVLIAINSVRMDSTASMNNLVYLSDTGEELLS
                     VRVNQEASARFINYQENTRSFVITYYDPQIVNGTLEIINVTDTGTVVWDKKMIFTGTL
                     NQVLNVHEGIVLAGTGILWKSDKTELRKNQPALFIARLNLNGEILGEKMVEGFRLEKA
                     YKVNDSNISLMSSLGAAEGPPGSTDFIITNSSLELIYPRKD"
     gene            14910..17279
                     /locus_tag="VGA21_02475"
     CDS             14910..17279
                     /locus_tag="VGA21_02475"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9649401.1"
                     /translation="MLKSFLTFWAKSILRNPLFSSIKLLGFALGIACTLLLIVFSANE
                     LTYDRFHENHKSLYRIIIKTESREGVTLSAVNTAAIAESLEREIPEIKSTVRFAFPFE
                     GFLKWNETSLKSTNLFYADSSLFSTMSFNLKLGNPKLALAEPYTMVLSESLAKNLFGN
                     QNPVGETVVLDEEKQFKVTGIIEDAPSNSQIRYQALLSFNSLLDQRLFLDWDGGWNYL
                     SYMMLHPKANPNDVIKKSMPIFDREINDRLREIGIYYSMLLEPITKSHLFSEAEFDLP
                     SKGSLDQIIILSVAGLFVLIMSCVNFISLSAVQATSRLKEIGIKKVIGIPNRRLFITF
                     MLETVLLTLLALVLASIVLLLLEPGIQVYLGFGIIELLKSNAYILAIIISLVVIIGFI
                     SGFYPAFILSRFKIADAVKGKFAKPGGLPVGIKVLILFQFTITIILFILTLVVYEQRD
                     FILNKDLGFRKENIVVLPLPNLETENKMETLKNELSGIASVVNICASSEIPGNGFTSN
                     GYFPEGHSEPMMFNVVDIDAKFLETLDVGIIKGRNFMANNIADREAYMVNESLAQKLG
                     WDNPIGKTITRNGDHQVIGVVKDFHFSTLHHPVEPLIITMKPWRGYNYLLIQITGDQY
                     SESVAQMANAWNKINPQETFDYFFLDQTFESYYDSERRLGEVFLFFSIIAIVLCGSGL
                     YATVSYSLKNRIKEIGIRKILGAGTLSLGFRLGGMQLIWILAANVIAIPAAMYLAELW
                     LSRFQFTAGIGLPLWLGGVIFSFSIGILISGVQTVKAVRSNPVDILRQE"
     gene            17474..19405
                     /locus_tag="VGA21_02480"
     CDS             17474..19405
                     /locus_tag="VGA21_02480"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX9649402.1"
                     /translation="MKHKLLFIISGIVLFACSQNDHKDLSHGNKFDDQVIREIYTLQD
                     LRDTETLINFLKQQDADYRREAAAALGSVQDSTSILPLINLLHDEFVDVRLAAAYSLG
                     QIKNPAAEEGILNALDDEKVPLVMRELWEALGKCSTSTGINFLVTHNSQDSLEKEGMA
                     WALYQIGLRGLANEETTSKAGSLLDFSNLYSTRLGASHYFSRTRNIDISNLEEKVAKS
                     ALQDMSPFVRMSSARALSKASDSIRTSTLHAIIRDEPDPRVIINAINALAGVEADKEV
                     IEKCLKHNNVNVVVAAGDFISRNIALFESQLIELIDITNNWRAKATLIGAAISTTDLQ
                     DQSNAAAIEAYRKSTNNYEKAALLTALGKNSKNHSLLWTEIENSSNKAMATAGISALA
                     SIRGSADYDISLDETFINYFRNAILTGDQALVAIASGALRNPDYGYKSKINDYSFLSV
                     AKEKLTLPMDNETLQSLERTIAYFESRESPPVINEYNHPINWEIVKSISNGQRAVITT
                     TKGKIVIELLVNESPGSVDNFYSLANSHYYDGKVFHRVVPNFVIQGGCPRGDGFGGED
                     YSIRSELGPLRYKEGSLGMASAGKDTEGVQWFITHSPTPHLNGSYSIFGQVIEGMNVV
                     NAIEVGDVIEKIEFPSIDE"
     gene            19398..>19574
                     /locus_tag="VGA21_02485"
     CDS             19398..>19574
                     /locus_tag="VGA21_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009183577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEX9649403.1"
                     /translation="MSEFYQNYKIGVLGGGQLGRMMIQAGIDFNLDIHVLDPDPDAPC
                     KSLASTFTKGSFTDE"
BASE COUNT         6134 a         3609 c         4107 g         5724 t
ORIGIN      
        1 ttttcacacg tttcctgtcc gcatttccat atggtaccct ggcggtaaat ttaatcggat
       61 gttttctgat aggactcttt tttggatttg tcgaaaagac cgatgtcaat cccgatttga
      121 gaatttttct catgaccggt atattaggtg gatttactac cttcagcgca ttttcaaatg
      181 aaaccgttga attgttaagg caggggagtt ttggctatgc aagcatttat gttctttcaa
      241 gcctcgtact tggtatattg ctgacttttg caggcatagt gctgtcaagg caattataaa
      301 ctgcattacc taacgtttcc agccctccac acctcctgat ctatagtaat tcccaagaaa
      361 aaacattcgg aattgtaatt gtttatttgg tcagtaagaa acacactcaa gccatcctat
      421 cgaataatag aatcgaattc ttggcgcgga tgcaactatt aagatattca ggcgtattta
      481 tattatatac aattaggaca attatggtca cttattcact tggagagttc gaagaagtag
      541 tacttttaac agtagctatt cttcaaaaga atgcctacgg agtgtccatc aaacttgata
      601 ttcagaagag attaaatagg aaggtcagtg ttggggctct tcagtcagca ttacgtcgga
      661 tggaacggaa gggatatata aaatcacact ttggtgaggc gactcccgaa agagggggta
      721 aaagaaagcg ctatttcgaa atcactttaa atgggaaaaa agcggtgagt catgctatgg
      781 aagaccggat tagcctttgg aaggccagcc cggaattgtt gacagaactg ggcagattga
      841 tatgatcagg cgcaactgga tatactcaat tataagacca tttatcagac ctgagttttt
      901 ggaagacata gaaggagatc tgaaagaatt gtatgaatca acaaatgcga ccaggcaagg
      961 attaattttt catttgaaat tcagctttga ggtatttaaa ctattacgtc ttgagctaat
     1021 taatattccc tttaatgttg tacccaaatt aaaattgtat atgattcctc attatttcaa
     1081 agttgcgttc agaagtttgt taaaaaataa acttacttct tttgttagcc ttgcaggtct
     1141 ttcattgggt ttggcatgtg taatgcttgc atatttatac gtaagtcacc atacaggatt
     1201 tgacgatttc cacaccagga aggatcgaat attcagggta gtaaatggag acatggattc
     1261 aggtgaaggg tgggtaaaaa ttgcggctcc actgttgcct caactaaagg aaaccattcc
     1321 ggaaattgaa tcctttacca gatttgaacc catttccagg ggagaaaaag ttttgattga
     1381 aagcgaaaat aagaaattca atgaacccta ttttgtaatg gttgatcctg cattttttga
     1441 aatttttgac tttcccctgc tcattggcga taaatcactt gtgctcaatg aacccggcaa
     1501 tgttgttatt accaagcgaa tccgggagaa acttttcgga gaagcaaatc ccattggcaa
     1561 ggttataaag gctgatgaaa ctcatgagtt tatcgtttcc ggagtaattg aaaatcctct
     1621 acttcaatcc catctcaggt acgatttttt aatttcattc gaaaacctta aaaatattta
     1681 cggagaaaat tcggtgaata gctggggcgc atataattat ttcggttaca ttcttctgaa
     1741 tgaaaaagcg gatccattta ttactgcgga aaaaatccgg gacttttctg taactacaga
     1801 tagaagcact tttaattttg atcaggtaaa cctccagtca ctttcagcaa ttcattttca
     1861 gcataaccgg ggcaatttgc ttccaggcat caatccggtt tatctgtgga tttttgcttc
     1921 catttcatta atagtcctaa tcatagcact cgccaattat gtgaatttgt cgattgcttt
     1981 gtcaataaga agagcccgtg aatatggagt aagaaaagct cttggagcgg caagagaaaa
     2041 tttaattatt cagtttttta gtgaaacatt aattgcaatg ttcatttccc tgatgcttgc
     2101 cgccttgttt atctacgcgg tattgcctcc ccttaacgcc atgctgggta catcaatgac
     2161 attaaaattc gacaaaccgg aattctggtt cgcgctaacc ggaattgtga ctctggcaac
     2221 gatcctggca tcatcttacc tggcaattat tgtctccgct tttaatccgg ttactatcct
     2281 taaaggtaat attaaagtta agaaaggttc aggcttgcaa aatattctcc ttggaataca
     2341 atttctgttg tccatagtac tcctcattaa tactttcatt ataacagacc aactcaatta
     2401 tattaaaacc cggaacattg gaatggctcc ggatggagta gtaaatatac cgctatacaa
     2461 caaattagat tctgttaagc tgaatacatt gaagaatgaa ttactcaaag tatcgggggt
     2521 taccggggtc tcggcatcaa cattcgttcc cggaagtgca agttggcatc aaacagtgag
     2581 ctgggagggg caaactccgg aagaacagga taatgctcaa atgtacctga tagcgacaga
     2641 taaagatttt atgagaacat taggcctgga acttgtgtat ggaaatttgg aagatattga
     2701 aaattcttcc acaccacaaa ttaaatatat tattaatgaa agtggaatga aattcctggg
     2761 ttgggaagac ccgggagaaa gaaaattaag cccattcggg cctggtaatt atggagaaat
     2821 tgcaggggtg gtaaaggact ttaatttcag atcactacac agtggaatgg atccattgct
     2881 attagcagtg tctgacaggt tcttccacga gcaattatca atcaaacttc aaatccctaa
     2941 tctcacttca tcactaaagg atattgaaaa gacatttgta tctgtgtcag gtgggcttcc
     3001 atttgaatac ttctttctga atgataaaat tgaacaatta taccaaacag aaaatgaaac
     3061 cggcaaactc atattttact cgactctgtt tgccttattg ctggccacaa tgggactgtt
     3121 tggtattatt tcctttatga tagaagataa gacaaaagaa attgccatcc gcaagatcct
     3181 cggcatatcg tttttccaaa tgattttcat gttttcaaag agatatttgc tggtgctttc
     3241 ggttgctatt gtatttgcat ggccattgtc atggtttgtt atgaataatt ggttgaacaa
     3301 ttttgcgtac agaaccggac ttgattttga accatttgta attggaggag ctgtagcttt
     3361 tctgatgata atcggagttg tgggtattaa aatgtttact atcaagaatc tggatccggc
     3421 gaaggctcta aagcaggaat aaccaaaatt gctgcttgtt tttacactgc attttcctac
     3481 actttcttat aatctcttgt cagccggcaa cgacaatgtc cttagatttg gggtttttat
     3541 tttcagaaaa aaattgttcc actttgagtg tttccaatag ttctattgtg gggactctgg
     3601 tcaaaataaa atagggatgt cttccttgaa taatgagata gtctccaggg ttaatttcac
     3661 actataaagg ggattcccgg agcattattt tagattaatt ccggaaatta aactgactaa
     3721 tgtcataaac atttcactta tctattttgt agcttgctaa acccttgaag ttctaaatat
     3781 acttgctaaa tgaaaagaat atccatatta atactagtaa ccatctctgc cggcattttt
     3841 cagggatgca tttttgataa agacggatgc accagcgcat gtgcatcaaa ctttgattct
     3901 tccgcggata atgatgacgg ctcctgcacg gggtgtacgg atccgactgc tacaaattat
     3961 tgtcccggag cagattttga caatggaact tgtattttac cctgtgaagc caatcaatca
     4021 ggtaaagttt atttcattaa caatagcaat tccaatagca cttatgatgt tatttgggat
     4081 ggggtgaaaa tattcacagt ggctcctggc cagcaatcgt caacaatcac agtaaccgcc
     4141 aatattcagc atacgttggt atttagatat accaatacct ccaataatgc atgtacgccc
     4201 agcacgccgg ttattcctca atgcgaaacc tggtggttta attgtaccgg ataaattgta
     4261 aaattattga ataaacccgg attgttgtca tttcacggct agactaataa aaacccttgt
     4321 ggcattgcgg gtacaactac caattagcgg ttaaaattgc atgggtaagt tttattggct
     4381 aaatgtcaat gaatattgaa atcttaacag gcattggagt tgtccagtta gaaaaaaagc
     4441 cctacgccta tggagatgcg tagggcaaaa aaccagtggg atgttttttt tctaaaccct
     4501 ggtacaaagt aataaatgat tacttcctgg tggttaaatc accttgaaga gtaactgccc
     4561 gtcaaattca attgatgtga ctttttgggg gaattgtaac taaagttcaa taatttaact
     4621 ttagtacttt ttagccattt atagctttaa aaattgaaaa ctattataga tttgcaacct
     4681 gttttttctt aaaacagggc gcttagctca gctggttcag agcatctgcc ttacaagcag
     4741 agggtcactg gttcgaatcc agtagcgccc acttcagaaa acacttacat tgatgcttca
     4801 gaactccact tctctgcggt gatccgtgat accagtggga ttaaaaactc ttcttccgcg
     4861 gtcggagctg atttacgcag aaatactctt ttctcccgca gattgcgcgg atttgcgcag
     4921 aaatccctga ataaattctc tgtgcggtga tccacgatat ccgcgggatt aaaaaacttt
     4981 tcttccgcag atcggcgctg atttgaataa attctctctg tggtgatctg cgatatgagc
     5041 gggattaaaa aaatcttctc cctcagatcg gcacagattt tcgtagaaaa acttttctct
     5101 cctgcggatt tacgcagaaa tccctgcata aattttttcc gcggtgatct gcgatatcag
     5161 cgggagaaaa aaacctttcc cgcagattga gcattagtta actccttttc aagcaaatca
     5221 ctttgtaata atcaccggca cgggaaaggt tgtccggtat tgagaaattc cagatttatt
     5281 ctctttaagt attgaatctc tcagctctgg attctttttg aagtcccaat ccgcaatcaa
     5341 gacaccattc aattcccatt ttacaaatga taactttcca aattcacttg taggattaac
     5401 tccactgggc ccataggtta ccgtcaaagt atcaatatcc ccatttccat attcaatata
     5461 caagggactt gccggccacg cgttgatctt aaaataattg acattattaa acatcccttc
     5521 gtgtccaaat tttctcctgt agccgttgtc gtttatccca taaaggtttt caattaaata
     5581 attctggttt gtttcaaaaa ggtccccttc taacgttatg aaattatagt aggcctgggt
     5641 ataacttggg tcgatcgctg gacatgagca cacgaaaaat ggtataagaa taattataat
     5701 tcttattaat gaattcgtta agctgtaata agttctcatt taataattaa cttctcggtt
     5761 cgggttattt cacctgaaaa ctgaacaaga tatactcctg aattcaattt cgttaagtca
     5821 acatggtttg tgccctttac taaattgaaa gttaacaatt ttttaccact tgatatctca
     5881 taacacggat gttaacactg aaggttgtgg gctgatcggg gcaaatggct gaggcccatt
     5941 aattgtatat ccactttgag tagtagcgaa atttggaata ttgtctaaca tgacagcaat
     6001 ctgtccgaaa gtcaaatcac gggcgcaatc gaaagttgca tagctcatta gatttgtagc
     6061 atctggattc caaagagctc cccagttatc attggtatcc agaaagggtt caaaactcaa
     6121 tggattccat ttcttccacc gagcagtttc atttgtttct gatgattcac ctgggcagga
     6181 aatcagccag aaaaacacag ttgagataat gacaatcttt ttcataatag aacctgaact
     6241 ctaactttaa cgagttaata ggttaattaa taacgcacca tttgatttca gagtccatta
     6301 ttaataataa cctccctgca ggaaatgcaa tgatgtgcag ttaccggctg gatgagttgt
     6361 tttcttcata aaatacaatg aataaaatca aaaaatccgg ttttgaattt agtgttaacg
     6421 ataaaaaata atttaaaaaa ccattagtaa tttaatattc cgtttgccat tcatattttt
     6481 gataaagaat ctgtattttt tatactcatt atacttaaaa ccgccccaaa tgaaaagaat
     6541 ccttcttttc acgttaattc taatctcaaa ccttgctctc gggcaggagt ggctgacatt
     6601 gtatcaacaa tcacaaactg attatgagaa tgataatctt gaagcggcct taaaaagcgc
     6661 gttaaaatcc ttgtcaatct acgagaaaga aaatgagaga aataattcca attattgcag
     6721 tattctaagg catttatcca ttataagcta ttctgccggt gagataaatt cagcaatccg
     6781 gtatgggaaa gacgaaattg aaattttaag aggtctggat gaagttgata aaaatacgct
     6841 ggccggagca attaacaacc tcgcgctttt tcaccaaact cttggtgagg ctcatgaagc
     6901 cgaatcgctc ctggaggaag tggtggaaat atacagcgtg attgaaaatt ctggaaatga
     6961 attgtatatc gccaggggca tgcttgcgat atcacaattc ggtcagcaaa agatggatga
     7021 agccggtgat aattttagag tatccattgg atttttcgca aatgccgatg acttgccttt
     7081 tgattaccta accattttat tgaactatgg aatatacctg gtcaccaaag aggaattttc
     7141 aacagcactc acacatttat ctgatgcaaa aaattattat gaccagttca agatggatgc
     7201 tactccggag tatgtactgc tgttgcgaaa tttgggaaga gcaaaggtga ataccggaga
     7261 tgctgagtca gcaacagcgg agttggaaag ggcgctggaa atttcaagaa atctttatgg
     7321 acaaaatgat cctgaaacga tttctataaa aaatgaactt gcattgcact atcaaaggtt
     7381 gggcaaaaca gaactcgcgg atgcgctgtc ttcagatacc gacattacca atctggatga
     7441 ggaatcaaaa cttgactatg caatttcaca aagtaatctt ggggccatca gacaatctga
     7501 cggagatttg ataaaagcgg aggaattata cctggaggcg attgagatat tttccattgc
     7561 ggaaaaagag gtaaacagct atttgaatgc gctccaaaat cttggcaggc tttaccaaac
     7621 aaagggagaa tttgcaaggg ccggagatca gtttcaaaaa gcgagggaaa tagccacacg
     7681 aacatatggt gaatcaagcc atttctatgc ggatgcactg gttaatattg ccgacttata
     7741 ccgtattaca aataatcaca ccgaaagcgg gcaattatac ctctctgcac taaatgtttt
     7801 tgagtcagat atagatagcc cacaaaagga aattgccaag gctcatgagg gattggcttt
     7861 gcactacctc agtatgggga atttcaataa atcacaggag aatttccttt ccgccattaa
     7921 catatattca aatacgggtg ataagaataa attgctgaca acaaaaagca attatgctgt
     7981 acttaagcag gcaatgggag aatacaaaac ggcagaaaca ttgttacggg aaatctatgc
     8041 agaagtaacc gctacaagga aaggaaccct cgaagaatca gcagccgcgg aaaatctggg
     8101 aactatcctt attgaagcgg ccaaatatgc tgaagcagaa gaatatttta agattgtttt
     8161 aagaataaga gaaggttatg gtaaatccac ccccgagtat gctaaaggca tcattaacct
     8221 tgcgaggcta aaagtgcggg aaggcaaata cacagatgca gaagcgcttt acaggaatgc
     8281 tcttgaaatt tattccggct ctcttggaaa taatgaccta agagtagcca atcttgaaaa
     8341 caatatggcg ctcatgtatc aatacatggg gaatatggaa gctgcagaat cccttttgga
     8401 acgatcatca gaaaaaataa aaaatcaatt aggtatcgac catccggaat atgctactgc
     8461 tcttgaaaat cgtgcgtcat tgttgcaaat tttgggaaaa cgtgaggagg caatgccatt
     8521 acttcagcaa gcccttcaaa ttgatgaaaa gacgctggga aggagtcatc ctaattatgc
     8581 atttaccctg cacaaccttg cttctatcta tttggatgaa aagaattata tgaaagctga
     8641 agaacttttt ctacaatcgg tagaaattga gaaaagtgtt cttggcgaag atcatcccat
     8701 atatgcagga actctcaata acctcggcgt tttgtatgaa aaacaggaaa attatgtaaa
     8761 ggccgagaat tatttcaatc tggcattaag cattcgggaa aaagtattgg ggaaagacca
     8821 ccctgattat gcatacagcc aatatggatt ggcggtgcta tatcttaaga tgggtaaatt
     8881 ggctgaggcg cgaactttat atgagaccgc tattgcaagc taccttgatc aggtacagaa
     8941 tttctttcct tccttaagtg agaaagagaa gagtgatttt tacaccaaaa tcaaaccggt
     9001 tatagaaaac ttcaaagagt ttgctattga atatgcagta aatgaggatg gcaatatgtc
     9061 ttctaaattg atcggggaga tttataatgt gcagcttgcc acgaaagctt tgttactgaa
     9121 tgccagcaac aaagtcagga atcgaattgc ttcaagcggg gatctgaaat taattgaatc
     9181 atttaataag tggattgaaa taaaagaaaa cctggccagg tattaccaat acaatcttga
     9241 gcaacttgat gcggaacaag tagacatcac caaacttgaa gaagtagcca accaattgga
     9301 aaaggagatc tctttgcagt cccaggtatt tgccagtgag ttcgacaagc aaccggtttc
     9361 ctgggaacaa ataaaaaata aactaacgga taatgaagcg gccatagaaa tactgcgcgt
     9421 tcagcgaaat accaccacgg acacagtcat gtatgtgggc ctggtaatca aaaaaagttc
     9481 tgattttcct aaaatggtta ttattaaata tggggaatcc ctggaaagca tgatgtataa
     9541 attctataaa aactctgtca cttttagagc tgaggatcct tactcataca atgctttttg
     9601 gaaagattta gatacagaac tggagggcat cagcagagta tatgtttccg cggatggagt
     9661 ttataataag gtaaatttaa atacgcttcg taattctgga tccggagaat ttattcttaa
     9721 tcagtatgaa attattttgc taagcaacac caaagaactg cttaccgacc tttcttctga
     9781 agcatttcgt acggccacat tgctgggtta tgcacagtat cagtctgttc aaccggaaaa
     9841 tccacaacca caaggtgtat ttcaggcatt gggatcagga ggctggctca aagagggaat
     9901 ccctgcactg gagggtacta aaaaggaagt agaaagcatc tcgggtttgt tgcaatctca
     9961 tggctgggaa ttcaaaaccc tggaaaccac taatgcttct gaatctgcag tgaaaaattt
    10021 aaaggggcag ggtcttatcc acatcgcgac acatggtttc tttttagaag atttgaagat
    10081 tgatgaatcc ggtgatggct ttggggtgca tatcaagaat tatgaactga atccgctttt
    10141 caggtcggga ttattattgg caggaagtgg tgaaacaatc ttcggcacag ccaatcaatc
    10201 tcaacaggaa gatggaattc taaccgcgta tgaagtaatg aacctatctt tggaaaacac
    10261 ggacttagta gtactttcag catgtgaaac cggcttggga gaaattcgaa acggcgaagg
    10321 agtttatggg ctccaaagag cattgattgt tgcaggagca aaaggactga tcatgagtct
    10381 gtggaaagta aatgatgaaa ctacccagaa gctaatgaca tatttttatc aaaactggct
    10441 ttccggaaaa acaaaactgg aagcatttaa agcagcacaa ttatctttga aaaatgagtt
    10501 taatgatcca tattattggg gtgcctttgt tatgattgga agataaatta tgtattgcag
    10561 aaaaataata tttgcaacaa gcgtgatgct atttgcatca ctaaacgcgc aatttactct
    10621 tggccagtca gcgatgcaga ttgtagcagg tctggataca agagacagca cggttattga
    10681 ttttgctcca tccatttcag ctgacgggaa aactatgatc tttcaatcga accgtggaat
    10741 aaatcgatcg cgttattttc ttttccaggc aaaactggat gattccggcc gttggggtga
    10801 tgtgacttcc ctggataata taaataattt tggcgacagc ctggatctga ttggcgggcc
    10861 cagtatttct tttgacggaa acacgctttt cttttttgca ttctttgccg gcggttttgg
    10921 ccaggaagat atttattatt ctgtaagaga tggcgaaacc tggagtgcgc caatcaatat
    10981 tggcggggta ataaacagca gcggttacga aggcttcccc tcaatttcgg cggatggaaa
    11041 aactttatat tatgttagcc agaattacaa cggtcccacc gaccgggaca tagagcggct
    11101 taaccaattt tgtaccgttt tgatgaaatc ggaacgtgat gaatcaggaa attggggacc
    11161 accgaagaag ctaccatttc ccatcaacct tgattgtgaa aaagcaccgc ggattatggc
    11221 agacaataaa acgctggtgt ttgcatcaaa caggcccgga gctgtgggtg gaataggtga
    11281 ctacgacttg tatcaaaccc ggcttaatat cctgggcgaa tggggacctc ctattccctt
    11341 gaactatgta aatacaatcc aatctgaaca attttgttcc atttcggcct ccggagaact
    11401 aatgtattat gtgcaggccg ggcgagatat tttttctgtt cctattccac aggaattcag
    11461 gcaatttgtt aacacaattg tccagggtta catcacggat atggatgata agtccggcct
    11521 tgcagcagct attattgtta ctgatgcctt gacatcagac gcaattatga caatgagtaa
    11581 taacccttct gatggtagat atacgctggt cctcgctgcg ggtaaaaatt ataatattga
    11641 agtgcgaaag caaggttatt caagctatac tacatcttac gacctttcca atgttacgac
    11701 ctaccgggaa ataagcaatg acattcaatt gttcagtgag gcaaagcttg cggtaagtgt
    11761 aagcgatgaa gaattactgg aaacgattac agccagtgta actatttcag aatctggcgg
    11821 cggcggtgtt ttgcaaatgc aatctcctaa aacaggcaga cttgagctta acctcccgct
    11881 gggtaaaaaa taccacgtgg aggttcaaaa agataatttt gaggacaaca gctttgaact
    11941 tgatttgacc ggcttggtaa tgtatagaaa ttatgaagag gatgtgccct taaaaccaat
    12001 aaaagttgaa gttgcgatca acgttgcaga ccttactaat aattcaaaag tgaaaagtcg
    12061 tgtgctaata agaaataaaa acagggatga aacaatagaa gttgagggaa atgaaatggt
    12121 ttcattgcgt gccggcgaca gatatcagtt ggaagtaacc agtgatcagg gatatgcatt
    12181 taattcaaca gaaattgatc tgacaaatgc tgcaagtaat cctgccatcg ctgctcaaag
    12241 tgtaaatgtt gaacttcaaa agcttgaggc caatgcaagc cttacgctta aagagataac
    12301 atttgagagt aactcattta acctatctga aatttccttt acagagttgc aaagggttgt
    12361 gaaattaatg cgtgaaaatc ccacacttaa agtggaaatt gcagcacaca ctgatgacat
    12421 tggttcagat gcatacaaca gattactgtc tcagcgcaga gcccagtcag ttgtgaactt
    12481 tttgatcgga aatgatatct cttcgcaaag atttgtttca aaaggatatg gagaatcaca
    12541 accactgata ccgaatgatt cagaggaggg acgagcgaga aaccgtagag tggaattaat
    12601 aattttagca atttaagcat gaagaagata attgtgataa gtcttttggt gctggcaggg
    12661 ttcagccttt cacctgctca acgaataaaa tataaggact tggttcctgt gctggattcc
    12721 ttaaaagagg atgaagcaat cccaatgctg atagaattat tgcagactga aagagataat
    12781 cccaatgcaa acttcaggtt gggtttgttg tatgccaaaa aatataaggc cactgatcca
    12841 ttgaaggaat ttgaaaagtc aatagaatat gcaaacagga caaagttgca aatgattaaa
    12901 tcgaaggcaa tcatcgataa tcgtgaggta aaccgcaaca gtggttggta tgcccctttg
    12961 gccaccgaag tcaagggtaa taaagtggaa tttatcataa ttcagcagca aattgataat
    13021 gaatacaacc tggcggatgc cttcacgaat aatattcccc ctgcttatgc aagctttacc
    13081 aaagcagttt ttaactatga taaagctaag aaaacctatg ccgatatagc gcagaaatac
    13141 gctagcatta aggaattatg tcttctatat aatcaggata ttgaaaccag gatgcgccaa
    13201 ttacaattcc agtatgattc aacgtattat tatttcaatc aatataatag tttgaaggca
    13261 ggaacaataa tagcatacga ccagaaattg gtggtcaagc caattaatgt ttatcgaaaa
    13321 gatggcctcg cgacccaaac agattttttc actcagaaaa tcgatatctg gaattataga
    13381 aagtgggcag aggaggtaat aaatgaagta aattccagtg ttgtcagttt aagagaaaat
    13441 cttcaatcta actacgaaaa aatctcaaaa agtgtggaag taatggaatc ggggacggat
    13501 gttgaacagt ttgcaactat tgataaacaa ttacttttca cccttgggag aatggattac
    13561 cgtaatgcgt tagttccaat gttacaatac aatcagtttt tgcaggaata tttatcaagg
    13621 aaacgggagt tatccggatt ggaatcggat acagctattg tcttgcagac aaaactacaa
    13681 ctctacacat tactgctata tgaaaatatt aaggctgatt cgttgctggc tgatttggtt
    13741 gaaaaaagtc aggacgatgc aaaattaaag cgccatgaag aattccttat tcaaaatttt
    13801 ggaagcccgg aaggatttaa aaaaatggct ttggatgaaa aacaaaaagt ggaagctgaa
    13861 tacgcaaatt ttgtaactct gatcaagcag gccgttacaa aggtatcaat ggcagaagaa
    13921 gtttcaggga atacaattac gcatcagcgc atgaacctct cattacttgc cagccctaat
    13981 ccagttgaca gcctggtgga taataaaccc gtcacggctc acacagagct taatccggat
    14041 ggcagcagat atttggctgg tgttatccga aggaatacca aaaccaggca tgttgaagtt
    14101 tttgtgtgca aagtggattc aaggtcgaga gtaagctgga tgaaattttt cgatctgcag
    14161 atggataacg aaaaaagcct ctccaatcat atcccaacag ccataagggc aactcaaaaa
    14221 ggagtactga ttgctattaa ttcggtaaga atggattcaa cagcatccat gaacaacctt
    14281 gtttacttat cagatacggg tgaagaattg ctatcagtaa gggttaatca ggaagcatcc
    14341 gccaggttca tcaactacca ggaaaatacc cgctcatttg taattacgta ttatgatccg
    14401 cagatagtta atggaaccct ggaaataatt aatgtaacag ataccggaac tgtagtttgg
    14461 gacaagaaaa tgattttcac cggtaccctc aaccaggtat tgaatgttca tgagggtatc
    14521 gttttagctg gcaccggaat cctttggaag tcggataaaa cagaattgag aaaaaatcaa
    14581 ccggcgcttt ttattgccag gttgaatttg aatggagaaa ttttgggtga aaaaatggtt
    14641 gaaggtttcc ggctcgagaa agcttataaa gttaatgaca gcaacatcag tttaatgagt
    14701 tcccttggcg ccgctgaagg gccgccaggt tctacagatt tcatcatcac aaactcctcc
    14761 cttgagttga tttatccgcg aaaggattga gcaacttatt catcaacggc cgggaaatga
    14821 tcgctatttc ctgataaaaa tcagttttaa aatcttgcct tatccgtaac ttttcattct
    14881 taaaacatgt tttgaagaat aataatgtta tgctgaaaag ttttttgact ttttgggcga
    14941 aatctatcct gcgtaatccg ctcttttcgt ccataaaact cctgggcttt gctttaggta
    15001 tagcctgtac gttgttgcta atcgttttct cagccaatga gcttacgtat gatcggtttc
    15061 acgaaaatca taaatcactc tatcggatta ttattaagac tgaaagccgg gaaggtgtaa
    15121 cactaagtgc ggtaaatacg gcggcaattg cagaaagcct ggagcgggaa attccagaaa
    15181 taaaatccac ggtacgattt gcatttccat ttgaaggttt tctgaaatgg aatgagacat
    15241 cactgaaaag cactaatctg ttttacgctg attcatcact cttttctaca atgagcttta
    15301 acctgaagtt aggtaatcca aagcttgctt tagcggaacc ttacactatg gtactttccg
    15361 aatcattggc caaaaattta tttggaaatc aaaatccagt tggtgaaacc gttgtgctgg
    15421 atgaagaaaa gcaatttaaa gtaaccggta ttatcgaaga tgcaccttca aactcccaga
    15481 ttcgttacca ggcgctgctc tctttcaact cacttttgga tcagagactt tttttggatt
    15541 gggatggtgg ttggaattat ttgtcttaca tgatgctgca tccaaaagca aaccccaatg
    15601 acgtcataaa aaaatcgatg ccaatttttg accgggaaat caacgacagg ctaagagaaa
    15661 taggaattta ttactccatg ttgctggaac ccattactaa gtctcacctt ttttcagaag
    15721 cagaatttga tctgccttca aagggtagcc tggatcagat aataatctta tccgtagctg
    15781 gattatttgt tctgattatg tcatgcgtta acttcatctc acttagtgcc gttcaggcca
    15841 cctcacgctt aaaagaaatt ggaataaaaa aagtgattgg cattcctaat cgaagattat
    15901 tcataacctt catgctggaa accgtattgc ttacgctgct ggcattggtt ttggcatcaa
    15961 tagttctctt gcttttggag cccggcattc aggtttatct cggctttgga attattgaat
    16021 tactgaaatc gaatgcgtac atactcgcca tcattatcag cctagtagta attataggtt
    16081 ttatttcagg tttctacccc gcctttattc tttccaggtt taagatagca gatgccgtaa
    16141 aagggaagtt tgccaaaccc ggaggattac cggtgggcat aaaagttttg atattgttcc
    16201 aatttacaat aactatcatc ttgttcattc tcactttggt tgtttatgaa caaagagatt
    16261 tcattctgaa taaagactta ggtttccgta aagaaaatat tgttgtttta ccattgccga
    16321 atctggagac tgaaaacaag atggaaacct tgaagaatga actttcaggt attgcatcgg
    16381 tagtaaatat ctgcgcatcc tctgaaatac ccgggaatgg gtttacctca aacgggtact
    16441 ttcccgaagg tcactccgaa cctatgatgt ttaatgttgt cgatattgat gctaaatttc
    16501 ttgagacact cgatgtcggg ataattaaag gccgaaactt tatggcgaat aacattgctg
    16561 acagggaagc ctatatggtt aatgagtcgc ttgcacaaaa gttaggctgg gataacccta
    16621 ttgggaaaac aattacccga aatggagatc atcaggtaat tggtgttgtt aaggattttc
    16681 attttagcac cctgcatcat cccgttgagc cattaataat cactatgaaa ccatggcggg
    16741 ggtataatta cctgttaatt caaattacag gtgatcagta ctctgaatca gtagcccaaa
    16801 tggcaaatgc ctggaacaaa ataaaccctc aggagacctt tgattatttt ttcctggatc
    16861 agacttttga atcctattat gattctgaac gccggttggg cgaggttttt ttattctttt
    16921 ctattatagc gatagtactt tgtggctccg gcctttatgc aacggtaagt tactctttga
    16981 aaaaccgtat taaagaaatt ggaatcagaa aaattttagg agccggtacg ctatcgttgg
    17041 gattccggtt aggaggaatg caattgatct ggattttggc agccaatgtg atagctattc
    17101 ctgcagccat gtacctggca gaactttggc tttccagatt tcagtttacc gcaggcattg
    17161 gactgccttt atggctgggg ggcgttatct ttagcttcag catcggaatt ctaatcagtg
    17221 gtgtccagac tgtgaaagct gtacggtcta atcccgttga cattttgagg caagagtaaa
    17281 tttcttacta aggtattaaa gggcatacat tcctaatcat tttcccaact attgcattac
    17341 aagcaaccct gcttcaaagt catttgggaa agcctgccag gcggtttaga aagggtcgtt
    17401 taactccgtt cctttaaaaa acctttacct tttgtatatt gctattctat atgcgatgtg
    17461 agaatttaat tctatgaaac acaaactttt atttattatt tcaggaatag ttttatttgc
    17521 ctgcagtcag aatgaccata aagatttatc ccatggaaat aaattcgatg atcaggttat
    17581 cagggagatt tatacacttc aggatttaag ggacaccgaa acattaatta atttcctcaa
    17641 acaacaggac gctgattaca ggagagaagc tgccgcggct ctgggttctg tgcaggacag
    17701 cacctcaatt ctaccactaa taaatttatt gcatgatgaa tttgtagatg tgaggttggc
    17761 tgccgcctat tcacttggac aaataaaaaa tccggctgcg gaagaaggaa ttctgaatgc
    17821 acttgatgac gagaaggttc cgcttgtcat gagggagtta tgggaagctc tgggaaagtg
    17881 ttcaacttct acaggtatta actttttagt cacacataat tcccaggata gtcttgaaaa
    17941 agaaggtatg gcgtgggcac tttatcaaat tggtctgcgc ggattggcca atgaagaaac
    18001 gacatcaaag gcagggtcgc ttcttgattt ttcaaatttg tattcaaccc gattgggagc
    18061 ttctcactac ttttccagaa cccggaatat tgatatttca aatttggaag aaaaagttgc
    18121 gaaatcagcg ctgcaagata tgtctccatt tgtcaggatg tcatccgcaa gagcactgtc
    18181 aaaggcatct gactcaataa gaacttcaac tttgcacgca ataattcgcg atgaacccga
    18241 tccaagagta atcataaacg ccattaatgc cttggctgga gttgaagcgg ataaggaagt
    18301 gattgagaaa tgtttgaagc ataacaatgt gaatgtagtt gttgcagcag gtgattttat
    18361 ttcaagaaat attgcacttt ttgaaagtca attaattgag ttaatagata taaccaataa
    18421 ctggagagca aaagcaactt tgattggagc agcgataagt actaccgatt tacaggatca
    18481 atccaatgca gcagctattg aagcttatcg taaaagcaca aataattatg aaaaggctgc
    18541 actattaact gccttgggta aaaattctaa aaatcattca ttgctttgga cagaaattga
    18601 gaattcttcg aacaaggcaa tggcaacagc gggcatttcc gcgcttgcat ctattcgggg
    18661 atcagcagac tatgatatct ctttggatga gacttttata aattattttc gaaacgccat
    18721 ccttaccgga gatcaggcat tggtggcgat agcaagtggc gcattacgaa atccggatta
    18781 cgggtacaag tcaaaaatta atgactactc ttttctttca gtcgcaaagg aaaaacttac
    18841 tttaccaatg gacaacgaaa cattgcaatc gttggaaaga acaattgctt attttgagag
    18901 cagggaaagc ccaccggtaa ttaacgagta taatcaccca attaactggg agatagttaa
    18961 atcgatctcc aatggacaac gtgcggtaat tactactact aagggcaaga tagtgataga
    19021 attgttggtg aacgaatcac cgggttcggt ggataatttt tattctctcg caaactctca
    19081 ttattatgat ggcaaggtct ttcatcgtgt tgtgcccaat ttcgtaattc agggtggctg
    19141 ccccagaggc gatggttttg gtggtgaaga ttattccatc cgctcagaat taggaccgct
    19201 tcgctacaaa gaggggtctc tgggaatggc ttcggccggc aaggatactg agggggttca
    19261 atggtttatt actcattccc caactccgca tttaaacggg tcttattcaa tttttggtca
    19321 ggtcattgaa gggatgaatg tagtaaatgc catcgaggtt ggagatgtca tagaaaaaat
    19381 cgaatttcca tctatagatg agtgagtttt atcaaaatta caaaattgga gttcttggcg
    19441 gaggtcagtt gggtcgtatg atgatccagg ccggcattga tttcaacctg gatattcatg
    19501 tgcttgatcc ggaccctgat gcaccttgta aatctttggc atctaccttt acaaaaggct
    19561 catttaccga tgag
//
LOCUS       DASWHX010000043         6834 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_2613419, whole genome shotgun sequence.
ACCESSION   DASWHX010000043 DASWHX010000000
VERSION     DASWHX010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 6834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6834
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2613419"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..499)
                     /locus_tag="VGA21_02490"
     CDS             complement(<1..499)
                     /locus_tag="VGA21_02490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX9649404.1"
                     /translation="MRTKIEFLISLMIFTVLILLINSVDELGGGWYVITLIPLLMMFA
                     IIDHSLKSQKRISRQNIELKKLVASVHALKEKYLLEMRGLTLEGRTSKSMFNELNAIK
                     KTLTRTAIWAVMNTGQKIVYVNKLYCEISGYKESELIGKHHTISNDGLYEAEFWEEIL
                     QKVRKG"
     gene            complement(483..1214)
                     /locus_tag="VGA21_02495"
     CDS             complement(483..1214)
                     /locus_tag="VGA21_02495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649405.1"
                     /translation="MIIFDVKTKYMVLVHIKNDFNKLVIGRYLQVTGYPFQMVESEEE
                     AYRVIRKEKVKILMVELESVETNHLDFTVKVKTLNPCLEPVIYAINQTTLTYQETEAL
                     GYDFLLTGPLKLSELKSVSDTYNLITKFGQLKTRLAALCEGNDIFQRELSSKLVLNCK
                     NIKGELKSALKENDVNQMRSLSHKSTTLLAILGVKELDSLLSDGLDYLGNANAFIPDQ
                     FYHDLDFTINSIVNNLSKTCNENQN"
     gene            complement(1284..2480)
                     /locus_tag="VGA21_02500"
     CDS             complement(1284..2480)
                     /locus_tag="VGA21_02500"
                     /inference="COORDINATES: protein motif:HMM:TIGR04390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YaiO family outer membrane beta-barrel protein"
                     /protein_id="HEX9649406.1"
                     /translation="MIKRLIPFLLLGAVNYPALSWQQDDFYLKARNFSSSGEFDLALQ
                     QIQAAKALGITGYDYLLLESLVYGWSGNLDSANSIIDGVIKKWPESREAKETKIKFQY
                     WSTNYPEAIQLINELDTASAEMLLLKINSLILSGRKSEVLRIFEENKELIEIPELNKI
                     HKTLKKESAKNQISVDLSFAKNYTTNQNWIWTDISYSRKFNKLTFIPAITIANLYDKT
                     GLEYRLDFYTPVFSTTYIHLNLESSPDPVFPDLTLGSTLFQTLPYKFEISAGTRFFKI
                     DSIWHYIGSGSLSKYLSKNWISYKIFIHLDNPGFAPTHCLIVRRYFRENFLELSMSQG
                     PVPLNIEGVNEFTKISSRIISLGYEFPAGNTGTLKANMAWQSDQISDRNRSNRFTLSL
                     GYRFAF"
     gene            complement(2486..3886)
                     /locus_tag="VGA21_02505"
     CDS             complement(2486..3886)
                     /locus_tag="VGA21_02505"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX9649407.1"
                     /translation="MEMIYNFWNIIMLLFGVSIIGWYFVLAIISIYDLTIYLRKNRFD
                     DGAILSSQFSPSVCLIAPAYNEEKNIVDSIKSLLSLRYNNLDLIIVNDGSKDQSLKKM
                     ISAFDLEISMEKFVNILTTKKIRGIYRSKNEALKRLIVIDKENGGKSDALNAGLNFSN
                     AEYVACLDVDSIVENDAILKMIKPFLEDSGVIASGGVVRIANACEIEDGRLIDPRFPK
                     SWLPRFQVLEYLRAFLLSRLAWSRFNGLMLISGAFGMFKRDIVVKSGGYNTNTVGEDF
                     EMVVRMRTYMHAKNLRYKVVYIPDPLCWTEAPSDLKILGRQRRRWTRGMMETLKTHKS
                     LFMNPRYKILGMLSFPFWVIFEWFAPIIEVTGFITFIIFLLLNMVDLQLFLLIMMAIY
                     LFAVLNSCLALFAEEISFHKYIRKKDTLIVLLTALLEPIFYHPLNIYWSIRGNLDFLT
                     GASGWGEMTRKGFSKK"
     gene            complement(3886..4893)
                     /locus_tag="VGA21_02510"
     CDS             complement(3886..4893)
                     /locus_tag="VGA21_02510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649408.1"
                     /translation="MPELKYILPYVIWTLAGMTLLLIICIFIIRTRIEMSKFFWGKAK
                     RIIEGIVLARLFDHQTAFDSNSRHAWSLRIVRKVPGIRKKMLEYILELKKNIAGKERV
                     KLNNLLYDFDLTSVVTTKLKSGNWSEITKGLNEIKEFQNGNLYDEVMPFLYSKNRRVK
                     MAAYRALIRLLPGSNLYFLCNTDLFLSEMDQIQLHYELTKRKLEIPEFESLLHSKNTS
                     VVLFAIKMISAFQQHSAVEKLTGLLDHEEEKIAIESLKALKELNAVGITDLLMFKLFI
                     IDSSPLLIAYLETLIALAHGRNFPLLEAYRWYPDDQIRAIFNSNIRHLTEQHNLLSKA
                     S"
     gene            complement(4886..5263)
                     /locus_tag="VGA21_02515"
     CDS             complement(4886..5263)
                     /locus_tag="VGA21_02515"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX9649409.1"
                     /translation="MKKILACDDDEVLLSLLKFKLSTPDCEVITFTDGKKVLKYIKKE
                     YFDLLLIDLYMPFYSGLEIISYLRKKLNSRKPVIVLSAEGIEQTMSNAFSIGADDYVT
                     KPFSIVELKARVTRYLNKDFSNA"
     gene            5507..6637
                     /locus_tag="VGA21_02520"
     CDS             5507..6637
                     /locus_tag="VGA21_02520"
                     /inference="COORDINATES: protein motif:HMM:NF022588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HEX9649410.1"
                     /translation="MRVHLLQKMTLPTMAMMISFGALAQLAHLPRNPSPAAEVSQTVG
                     ISKITVNYSRPHVTTPGGQDRTGTIWGQQVPYGWSDPIPGFGSGNKIPWRAGANENTI
                     ITFSDDVKIGGKDLAAGSYGLFLLVEENGTATWIFSKNTQSWGSFFYDEKEDALRVTV
                     QSEDHDQTPRLTYNFTDLTGNSVTIALDWEKKRFPLKVEFDMHAIVLASITDELRSTA
                     GFTWQSYNQAANYCLNNNVGLEQGLKWAEASIANTSNFTNIGTKSQLLTKLGREAEAK
                     EIMTAALENPSATEGDYYNYGRQLIGQDKDQEALAVFEKLNKKWPNHWLAPHGLARAY
                     SALGDYKKALKYERVALERAPAGSKGFLEGFVKTLEEGKDFN"
BASE COUNT         2041 a         1372 c         1227 g         2194 t
ORIGIN      
        1 ttcccttcct caccttttgc aatatttctt cccaaaattc cgcttcatat aatccgtcat
       61 tgcttatcgt atgatgtttt ccaatgagtt cagattcttt atatccggag atttcacaat
      121 ataacttgtt cacatacaca attttctggc ccgtattcat aaccgcccat atagctgttc
      181 tggttagggt tttcttaata gcgttaagtt cattaaacat ggatttactc gttcggccct
      241 ctagtgtcag acctcgcatt tccagtaaat acttctcttt caaagcatga accgaggcaa
      301 ccagtttctt taattcaatg ttctggcgac taattctttt ttggcttttc aggctgtgat
      361 caatgatggc aaacatcatc aataaaggaa ttaatgttat tacataccag ccgccaccca
      421 gctcgtccac agaattaatc agcagaatta atactgtaaa gatcataagg gaaattaaaa
      481 attcaatttt ggttctcatt gcacgttttt gataaattat ttactatact atttatggta
      541 aaatccagat cgtgatagaa ttgatccgga ataaatgcat ttgcatttcc caggtagtca
      601 agtccgtcgg aaagcaaaga gtctaactct ttaactccca gaattgccaa aagggttgtt
      661 gatttatgac tcaggctcct catttgattt acatcatttt ctttcaacgc acttttcaat
      721 tcccctttaa tatttttgca attcaaaact aattttgacg ataattctct ttgaaatatg
      781 tcgtttcctt cgcacagtgc cgctaacctt gtttttaatt gaccgaattt agtaatgagg
      841 ttataggtgt cagaaactga cttcagttca ctcaatttca atggccctgt taatagaaaa
      901 tcataaccca gtgcttccgt ttcctggtat gtaagtgtag tttgattaat tgcataaatc
      961 acaggctcca aacatggatt caatgttttt acttttaccg tgaaatcaag gtgattggtt
     1021 tctactgatt ccaattcaac catcaggatt tttacttttt ccttacgtat aaccctataa
     1081 gcttcctctt cactttctac catttggaat ggatagcctg tcacctggag atatctgcct
     1141 ataacaagtt tattaaagtc attttttatg tgcactaata ccatatattt cgttttaaca
     1201 tcaaatatga tcatttaatc acctggattt ttagcgctat tccctaatta attgtgatag
     1261 gtttttcact taagaaaatc ttgtcagaat gcaaacctgt agcccaggga aagagtaaac
     1321 cgattggatc tattcctatc ggaaatttga tccgattgcc atgccatgtt tgctttcagg
     1381 gttccggtat ttcctgcagg aaattcataa cctaaactta ttatcctgga agaaatcttt
     1441 gtgaattcat taaccccctc aatattcaat ggcacaggtc cttgcgacat tgaaagttcc
     1501 agaaaattct ccctgaaata tcttctcact atcaaacagt gggttggagc aaatccgggg
     1561 ttatccaggt ggatgaatat tttatatgaa atccaatttt tagataaata ttttgacaag
     1621 cttcctgaac ctatataatg ccagatcgaa tctatcttga aaaacctcgt gcctgcagag
     1681 atttcaaatt tatagggcag agtttgaaaa agagtggaac ccaatgttag atcgggaaat
     1741 acgggatccg gtgaagattc aaggttcagg tgtatataag tggtagaaaa aaccggagta
     1801 taaaagtcta acctgtattc caatcccgtc ttatcataca aattagcaat agtaatagcg
     1861 ggaataaatg tcaacttatt aaactttctt gaataggaaa tgtcggtcca gatccaattt
     1921 tgattcgttg tataattttt tgcaaaactt aaatcaaccg aaatttgatt tttagctgat
     1981 tcttttttaa gtgtcttatg tattttattt agctccggga tctcaatcaa ttctttattc
     2041 tcctcaaaaa tccttagaac ttctgatttc cttcctgaca aaataaggga attgattttt
     2101 aataacagca tttcggctga tgcggtatcc aattcattaa tgagttgaat tgcctccgga
     2161 taattggtgc tccagtattg aaatttgatt ttagtttctt tcgcctccct gctttccggc
     2221 catttcttaa ttaccccgtc tattatacta tttgctgaat ctaaattgcc actccaacca
     2281 taaaccagtg attccaaaag gagataatca taacccgtta tgccaagagc tttggctgct
     2341 tggatctgct gcagcgccag gtcgaattct cctgaagaag aaaaattccg tgcttttaaa
     2401 taaaaatcat cttgttgcca ggataaagca gggtaattaa ccgcacccag caacagaaaa
     2461 ggtatgagtc tcttgatcat cgttttcatt tttttgaaaa accctttctt gtcatttcac
     2521 cccacccgga tgcccctgtc aaaaagtcca ggtttcctct tattgaccag tagatattca
     2581 ggggatgata aaatattggt tcaagaagcg cggtaagcag cacgatcaat gtgtcctttt
     2641 tcctgatgta tttatgaaat gaaatttctt cagcgaacaa tgcgagacaa gagtttaaaa
     2701 cagcaaagag gtatatcgcc atcataatca gcaggaaaag ctgcaggtcc accatgttca
     2761 gcaaaaggaa tatgataaaa gttataaatc ccgtaacttc aataattgga gcaaaccatt
     2821 caaaaattac ccaaaaaggg aaacttagca tccccagaat cttatatcgt gggttcatga
     2881 ataaagattt gtgggttttc agggtttcca tcatgccgcg agtccatctt ctcctttgcc
     2941 tgcctagtat tttcaggtca gaaggggctt cagtccagca caagggatcg ggtatgtata
     3001 ctactttgta gcgtaagttc tttgcatgca tataggttct cattctaact accatctcaa
     3061 aatcttcacc caccgtatta gtattataac cgccgctttt gacaacaata tctcttttaa
     3121 acattccaaa tgcgccggaa ataagcatga gtccattgaa tctgctccag gccagcctgc
     3181 ttaaaagaaa cgctctcaga tactcaagta cctgaaacct tgggagccag cttttcggaa
     3241 atcggggatc aattagccgg ccatcttcaa tttcgcaggc attagcaatt cgcacaactc
     3301 ctcctgatgc aattactccg ctgtcttcaa ggaatggttt tatcattttc aggatggcat
     3361 cgttttccac aatgctgtca acatccagac aggccacata ttcagcgttt gaaaaattca
     3421 gacctgcatt caaagcatct gatttgccac cattttcctt atcaataaca attaatcttt
     3481 ttaatgcttc attttttgac cggtaaattc cccgtatttt ctttgttgtt aagatattaa
     3541 caaatttctc catggagatt tccaaatcaa acgcgctaat cattttttta agagattggt
     3601 ctttgcttcc atcattcaca attatcaaat cgagattatt atacctcaat gaaaggagtg
     3661 attttatgct gtctacaata tttttttctt cattataagc aggcgcaatt aagcataccg
     3721 atggcgaaaa ctgcgatgaa agtatagctc catcatcaaa cctgtttttt cttaagtata
     3781 ttgtgaggtc ataaattgaa ataatcgcta aaacaaaata ccatccgatg atgcttactc
     3841 cgaacagcaa cattattata ttccaaaaat tgtatatcat ctccattagc ttgctttact
     3901 taataaatta tgttgttctg tcagatgtct tatattacta ttgaaaatcg ctctaatctg
     3961 atcgtcagga taccacctgt atgcctctaa caatggaaaa ttacgtccgt gtgccagtgc
     4021 tattaaagtt tccagatacg caattaataa tggtgaggaa tcaatgatga ataatttaaa
     4081 catcaacaaa tcggttatac caactgcatt gagctcttta agagctttta gagattcaat
     4141 tgctattttt tcttcctcat gatcaagtaa accggttaat ttttcgactg ccgaatgttg
     4201 ctgaaatgcg gatatcattt ttattgcaaa tagaacaacc gaggtatttt ttgaatgaag
     4261 tagtgattcg aattcaggaa tctctaattt tctttttgtc agctcatagt gcaactgaat
     4321 ctgatccatt tctgacaaga aaaggtctgt attgcatagg aaatagagat ttgaacccgg
     4381 tagcaacctg ataagagcac gatatgcggc catttttact ctcctgtttt ttgaatacaa
     4441 gaatggcatt acctcatcat ataaatttcc attttgaaat tctttgatct catttaaacc
     4501 tttggtaatt tctgaccaat tcccggattt aagcttcgtt gtgactacgg aagtcaggtc
     4561 gaaatcatac aataaattat tcagcttcac cctttccttt ccggcaatgt ttttcttaag
     4621 ctcaagaata tattccagca ttttcttccg gatgcccgga actttcctta caattctcag
     4681 ggaccatgca tgtcgggaat ttgaatcaaa agcggtttga tgatcaaaaa gtcttgccaa
     4741 aacaatacct tcaataatcc ttttcgcttt accccagaaa aatttgctca tttcaattct
     4801 tgttcgaatt atgaatatgc aaataatcag cagcaaagtc attcctgcca acgtccagat
     4861 aacatagggt aatatatatt taagctcagg cattggaaaa atctttgttc aggtaccttg
     4921 taactcgtgc ttttaattct acaatgctga aaggttttgt aacatagtca tcagcgccaa
     4981 tcgaaaaagc gttggacatt gtttgttcaa ttccttccgc ggataatact atgaccggct
     5041 tcctggaatt taatttcttc cttaaatagg aaataatttc aagcccggag taaaatggca
     5101 tgtatagatc gataagcaag agatcgaagt attctttttt tatatacttc agaacctttt
     5161 taccatccgt aaatgttatc acttcacaat ccggtgtgga aagtttaaac tttaatagag
     5221 ataacaatac ctcatcatca tcgcaagcca ggatcttttt cattttaata tgttttcact
     5281 aaatgtacat tggtaaactt tgcaaaaaac cggcgtaatg cttattaagt tagattaggt
     5341 atttcaccta tgattggtgg tcgcacttcc agcctttagt ttgcttcagc cgcagtacga
     5401 agcaaaaaat gacctgaaat caaacttaac aggcatatta atcgtaaaag gtaatatcgt
     5461 ttatcaacca ccaacctttt aaaattcacc ttttaaccca taatttatga gagtacatct
     5521 tttacaaaaa atgactttgc caacaatggc aatgatgatt agttttggcg cgctggctca
     5581 attggctcat ttgccaagaa atccgagtcc ggcagcagaa gtatcccaaa ctgtggggat
     5641 aagtaaaatt actgtcaatt attccaggcc tcatgttaca acacctggcg ggcaggatcg
     5701 tacgggaact atctggggac agcaggttcc ctacggttgg tcagatccca ttcccggctt
     5761 tggctcagga aataaaattc cctggcgtgc cggagctaat gaaaatacaa ttattacatt
     5821 cagtgatgat gtaaaaattg gcggcaaaga tttggctgcc ggttcttacg gcttgttcct
     5881 tttggttgaa gaaaatggaa cagcgacctg gattttcagt aagaataccc aatcatgggg
     5941 tagtttcttt tatgatgaaa aagaagatgc gttgcgtgta acggtgcagt ctgaggatca
     6001 tgatcaaacc ccaagactca cttacaattt tacagacctt accggaaatt ctgttactat
     6061 agccctggat tgggagaaaa agagattccc tctgaaggtt gaatttgata tgcatgcaat
     6121 agttcttgca agcattaccg atgaactgcg aagcaccgcg ggtttcacct ggcaaagcta
     6181 caatcaggcg gcaaactact gcctgaataa taatgtaggt ctggagcaag gattgaaatg
     6241 ggcagaagca tcgatagcta atacttccaa tttcacaaac atcggcacaa aatcgcagct
     6301 gctgactaaa ttgggccgtg aagcagaggc caaagaaata atgaccgctg ctttggaaaa
     6361 tccttctgca acagaaggag actattataa ttatggccgg caacttatcg gccaggataa
     6421 ggatcaggaa gcattggcag tttttgaaaa actaaataag aaatggccga accattggct
     6481 ggctccgcat ggccttgcgc gcgcatattc cgcgcttggt gattataaga aagctttaaa
     6541 gtatgaacgt gttgccctgg aaagagcgcc tgccggcagc aagggtttcc tggaaggatt
     6601 tgtaaaaacc ctggaggaag gcaaagactt caattagttt tcgttgtaaa attgctttaa
     6661 tgaaatccct ggcagaagcc ggggattttt ttattgataa ttgttctttt ctgaaaaaat
     6721 tccctggtac ctgattatta tccctttctt gtggtaactg attggattct cggcggcttt
     6781 gcaactttcg atttatgaag acttcgggtt ggtatttgca ggattgaccg gtgg
//
LOCUS       DASWHX010000044        58715 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_773333, whole genome shotgun sequence.
ACCESSION   DASWHX010000044 DASWHX010000000
VERSION     DASWHX010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 58715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58715
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_773333"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1207..2607
                     /locus_tag="VGA21_02525"
     CDS             1207..2607
                     /locus_tag="VGA21_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020532526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEX9649411.1"
                     /translation="MQDIFELYRQKRKMQFKSINPYNNGVLQAFDLFPETRWKKSLEI
                     SEEVYKSWRKTSFQQRADLFFRLSNLLKEKRVETAKLMSLEMGKVHKEAVAEIEKCAW
                     VCEYYAENAEKFLHSESIDSTADKSLVRYDPIGAVLAIMPWNFPFWQVFRFAAPSLMA
                     GNVALLKHAPNVNMCSLTIEDLFTEAGFPAGVFQSLIVDVDVVEKIINHNIVQAVTLT
                     GSERAGASVASIAGSNIKKSVLELGGSDPFIVLADANLDNAARTGATSRMLNAGQSCI
                     AAKRMIVVESVHDEFVEKFRNEINKIKVGDPLAEGVTMGPMARMDLAESLEKQLKASL
                     EKGAKLELGGSRNNCIFEPTLITNVKPGMAAFDQETFGPLATVIKARDEAEAVELANK
                     TEYGLGASLWTTDLTRAEQLSAEINSGSVFINAMVKSDPRLPFGGIKKSGYGRELSHH
                     GIKEFVNAKTVFRVDS"
     gene            2805..3761
                     /locus_tag="VGA21_02530"
     CDS             2805..3761
                     /locus_tag="VGA21_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010611596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX9649412.1"
                     /translation="MHREKIRQVYDEVGRVVVGLRGLVNRLMIGLFTQSHILLEGVPG
                     IAKTLTVSTLARVLHLDFQRIQFTPDLLPADLIGTMIYNQKQGNFEVKKGPIFANMIL
                     ADEINRSPAKVQSALLEAMQEKNVTIGETTFELDRPFFVLATQNPVEQEGTYPLPEAQ
                     IDRFMMKVNVGYPSKDEEMEVMRRMSNMKFDDSVNAVLSKDDIFSIRDEINQVTISES
                     LEKYIIELVYATRHPKEYGLTEAANYIQFGASPRASINLNKAAKAIAFFEDRDYVLPE
                     DIKDVAMDILNHRLIMNYEAEADGVSSVDIITTVLNKIPINR"
     gene            3889..5109
                     /locus_tag="VGA21_02535"
     CDS             3889..5109
                     /locus_tag="VGA21_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013454925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649413.1"
                     /translation="MKKITHLLLIPFCFALVFISCEEDKGGIGGETGAENEILVEANI
                     SGNEVWSSNFIYILAGRITVLDGATLTIEPGTVIKGQAGTGSNATALMVARGGTLIAD
                     GIATAPIIFTSIADEINPEQVAAGDFKSPNLDPDIDGLWGGVIVLGKARISASNSSGD
                     VSEVQIEGIPTSDSNGLYGGNEDNDNSGILRYISIRHGGANIGSGNEINGLTLGGVGS
                     GTTVEYIEVVANQDDGIEWFGGTVNTSNVVVWNVGDDGIDTDQSWSGTLNNFVVITPT
                     GHCFELDGPEGSYEAGHTIQNGTIIASTDDGSRFSEHLINLDNNSIVSLSNLYFFNLV
                     EGQVINRIQCVDGNCPFSGIQLLVPDGNVLEDFVYGGVVPAGIIEVSTPSVGASSALF
                     AWTWAAQVGMLNGL"
     gene            5245..8121
                     /locus_tag="VGA21_02540"
     CDS             5245..8121
                     /locus_tag="VGA21_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014020296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX9649414.1"
                     /translation="MYRLTRIALAFLSTTLPFFAAAQGIVRGIIIEDKNGEGLIGVTV
                     VVKGTTTGAITDFDGNFEIKLDPGEYDLQVSFISFETVTITGVLVQTGKVTLLNGIRL
                     KEYVKELEGVTITADVIRNSESALLTVKKKSPNLIDGISSVNFKKIGDSDAAGAVKRV
                     TGVSVEGGKYVYVRGLGDRYTKTTLNNVDIPGLDPDRNSMQMDIFPTNLIDNIMVMKS
                     SLAEMPADFTGGAVNIETKDFPDEKIFDISLGIGYNPGMHLRPDYLVYEGGKTDFLGF
                     DDGTRSLPGEARNNPVPSPVSGDSQQDVNAFLNKFNPTLGAKSKTSFMDYSLGVSFAN
                     QTSLKGDKKLGYILSTTYKSSTKFYDEVIYGEYQRLINPSLYELRYATIQNGSVGEHN
                     VLLGGLGGIALKTKNSKLKFTIMHLQNGESKAGQFFVDNDGEAVGQSGYFASSDNLEY
                     TQRGLTNLLLNGEKHSINTLWKLDWSVSPTFSKITNPDIRKTAFTQTVVDTLFSAGAG
                     GNPNRIWRYLEELNLVSKAAVSKDYKLFGESAKLKFGASHIFKMRDYEILGFDIQFFG
                     TQPDFNADPNNVLNNTNLFPNGTLYYSSGNNIPNPNQYYSSVHNTGIFVSNEFSPLAN
                     LKAIIGLRGEKYVQRHTGRDVEYASLGTGNNLDNEKVLDAFDIFPSLNLIYSLNDQQN
                     LRFSYSRTIARPSFKELSFAQIIDPITNRIFNGGLFTYPDWEGKLTETSIDNFDLRWE
                     FYIKRGELISASAFYKTFDDPIELVRIPEQQTSTEYQPRNVGDGRLFGAEIELRKNLD
                     FFAPSLENFNISGNFTYVRSQIDMTNTEFNSRKTYEKDGQEIENVRVMAGQAPYIINA
                     GFSYNNPVVGLDGGFFYNVKGATLVIVGAGLFPDVFAKPFHSLNFNMNKTLGSDQRSI
                     ITLSISNLLNSSRKEVYSAFRAKDQNFTRFEPGTTISVGWKYSF"
     gene            complement(8298..8675)
                     /locus_tag="VGA21_02545"
     CDS             complement(8298..8675)
                     /locus_tag="VGA21_02545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649415.1"
                     /translation="MLGKLLKFGLVTIFFLLLIVASCNDRRHISPQEIVEINVNLTSK
                     EIYQYDLGEIPIEGGFSIIKVAKNSRICTIKLSNKNKWVFEYQAIADFAGNDFVELRN
                     CISAGGSECSQIIHYQFYFSIER"
     gene            complement(8761..9453)
                     /locus_tag="VGA21_02550"
     CDS             complement(8761..9453)
                     /locus_tag="VGA21_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020526558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX9649416.1"
                     /translation="MRKKKYKILVVDDEPDIIEILQYNLEKEGFEVRSLIDSRETLTA
                     ALEFKPDLAILDIMMPYQDGVETCRLLRQEKELSDTYILFLTARTEEYSEIAAFETGA
                     DDYLIKPIRPRALISRINSLLTREVKRIHTAPLDNGNLVIDKMSYTVHVGDRIISLPK
                     KEFELLHFLAENPRKIFNRDALLDKVWGLDSQVLSRTVDVHIRKIREKIGDEYIATIK
                     GVGYKFEPEGKE"
     gene            9821..10090
                     /locus_tag="VGA21_02555"
     CDS             9821..10090
                     /locus_tag="VGA21_02555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649417.1"
                     /translation="MKQRMSPTSPQSLKAQVRKAALRAYADLLIDDPSDITDQHFKIL
                     LKDMSPGEIIYATSRILGKESDMVNMVIGRFNNYMEKAGSAIVNI"
     gene            complement(10142..10837)
                     /locus_tag="VGA21_02560"
     CDS             complement(10142..10837)
                     /locus_tag="VGA21_02560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649418.1"
                     /translation="MKFTTYIITFFLNFSFVFAQEITVKEESKAIEKVTYAGYETTVN
                     GSVEKHRDAWTKLLKNKGKVRKKNSLYFLEDFVFPQITDKLLTGVTQINQSRDSIVTI
                     WLGIDSSVLEEEESGKLMNEISGMLYTYVLDYKKSLVLNDIQNAERAAAFTSRQHQRL
                     LQNLENLTLRLLDAENEKRRLEESVKTMELEIEVLKQRIENNKADQEKTYEELEQIRK
                     VLEMHKERLKKLN"
     gene            10921..11823
                     /locus_tag="VGA21_02565"
     CDS             10921..11823
                     /locus_tag="VGA21_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009582072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HEX9649419.1"
                     /translation="MPKKVLIAGGSGSIGTQLTKLLLEKNFSVSWLSRENKTTEGVKT
                     YLWNYEENIIDDNALNNTEIIVNLAGAGIADKNWSKERKKILKESRIKTTNLLLNKIE
                     EKPNDVKTYISASATGYYGYDSGSIEKKEGSRFGDDFLATLTKEWEAAADGFSSKGIR
                     TVKLRTGFVVGNNQNGWEKIAKVVKTGFGAAVGSGDQYMSWVHIHDLCRIYLWAIENE
                     KAEGVYNAVSPNPVTNKEFTRQAAQALKRPFFMPNVPGFILRLMYGEIASALLGSSRI
                     SSEKIQNEGFQFDYPTLTEALREI"
     gene            11940..12692
                     /locus_tag="VGA21_02570"
     CDS             11940..12692
                     /locus_tag="VGA21_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009582073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEX9649420.1"
                     /translation="MKEEANESIKTNGADEDGESKIRNAFKDRDWNEIKSSDSWAIFK
                     VMSEFVQGFEKLSQIGPCVSIFGSARTKPGAPYYKMAEEIAAKLVRHGYGVITGGGPG
                     IMEAGNRGARSESGKSVGLNIELPHEQFSNPYIDSDKLISFDYFFVRKVMFVKYAQGF
                     VVMPGGFGTLDEMFEAVTLIQTKKIGRFPIVLVGKEYWQGLFDWIKKVAIEESANVSP
                     EDLELVNIVDNATDAVKVIDEFYSKYLLSPNF"
     gene            12701..13627
                     /locus_tag="VGA21_02575"
     CDS             12701..13627
                     /locus_tag="VGA21_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013454619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEX9649421.1"
                     /translation="MKYINTYLSLATLIILIGYIFYDHAEVEKETPARITVAEPDWNF
                     PGGAKSFDLPDTISFAGERVPLEIPDVRERLDREIHINTYWHNNTIFMIKRANRWLPQ
                     IEKILREENIPDDFKYIAVIESGLINDISEKNAVGFWQFLKGTAKDFGLTVNRDVDER
                     YDPIKSTRAACKYFKQAYDKFNNWTLVSASYDRGMRGMQNAMDNQKVDSYYDLLLNEE
                     TSRYIFRVLALKEILSHPAKYGFQVKDIHLYEPEPTKTILVSEDIPDLIEFARTQGIN
                     YKILKRLNPWLIRDQLSVSRGESFEIAIPATN"
     gene            13754..16567
                     /gene="uvrA"
                     /locus_tag="VGA21_02580"
     CDS             13754..16567
                     /gene="uvrA"
                     /locus_tag="VGA21_02580"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012929517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEX9649422.1"
                     /translation="MEIFGAREHNLKNIDLKFPRNKLVVITGISGSGKSSLAFDTIYA
                     EGQRRYMESFSAYARSFIGNLERPDVDKINGLSPVISIEQKTTSRNPRSTVGTVTEVY
                     DFMRLLFARASEAFSHVTGNKMIKQTEDQILEKILEQFKSKKITILAPVVKGRKGHYR
                     ELFLQIRKLGYNKVRIDGELHDLVPKMQVDRYKIHDIEVVIDRVEVKDKEKFRINQSL
                     NLAMKEGKGVIMLMEENGSTHYFSKFLMDPESGVSYDEPAPNLFSFNSPYGACQVCNG
                     LGAVEEITRESVIPDSNLSISRGAILPLGVYRDIWIFKKIDAILKRYKFNLTTPVKNI
                     QDEVVDILLYGDKVPVAVPSVKYPGTEWNTKFEGIINFLVKQKDTGTEAIQKWINEFT
                     ISKTCPECEGARLKKESLCFKIDGKNIAELATYDLKQLADWFRNLEDRLSESQKRIAS
                     EVLKEIRKRLGFLTGVGLEYLQLNRTLRSLSGGEAQRIRLATQIGTQLVGVLYILDEP
                     SIGLHQRDNIRLIHALQSLRDLGNTVIVVEHDKEMMVSSDYLIDIGPGAGRHGGEIVA
                     QGKPLQFLKNGSITAAYLRGDLEISTPEKRRSGNGEKIILIKASGNNLNDLTVEFPLG
                     KMICVTGVSGSGKSTLIHDTLFPILNQHFYSSRLEPLPFAGVKGLEKIDKVIEVDQSP
                     IGRTPRSNPATYTGVFSDIRSLFSELPEAKIRAYKPGRFSFNVKGGRCETCEGAGLKL
                     IEMDFMPNVHVPCETCKSKRYNRETLEVRFKGKSIADVLDMTVDDAVIFFENQPRILR
                     KIKTLEEVGLGYISLGQHATTLSGGEAQRVKLATELSKKDTGKTFYILDEPTTGLHFQ
                     DIRHLLDVLNQLVDKGNTVLIVEHNLDVIKVADYIIDLGPEGGEEGGRIVALGTPESL
                     VKSGKGYTSKYLKSELKNKSATV"
     gene            16691..17995
                     /locus_tag="VGA21_02585"
     CDS             16691..17995
                     /locus_tag="VGA21_02585"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014796683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX9649423.1"
                     /translation="MNSQKLSIPDTTKKRIIIIGGGFAGINLLKNLKHQNFQLVLIDK
                     NNFHTFQPLLYQVATAGLEPDSIAGPLRKQISGNYEYYFRMASVLDIDPVTKKVLTDL
                     GEINFDILIIATGTVSNFYGNKSVEQNAFPLKEVNDALNLRSHILQNFEHAVLIDDQE
                     ELEKYMNIVVVGGGPTGVEVAGALGELKRHVLPKDYPELDFSGMNIYLVEGLEKLLAG
                     MSEKAGTTAVKSLRKFSVNVVLNTMVKEFDGNRVLLSNENAIETRTVIWAAGVKGTIF
                     NGISKESQLKGRILVDRFNKVRGTENIYAVGDISVMQTQAYPDGHPMLAQVAIQQGRL
                     LGRNLKRIEKSLNPVPFEYKDKGSMATIGRNKAVADLPGNMHFGGLIAWMIWMFIHLV
                     SIIGFRNKIVILSNWVWNYFTYDRGTRLIIRKFIPNWEKKSS"
     gene            18050..19102
                     /locus_tag="VGA21_02590"
     CDS             18050..19102
                     /locus_tag="VGA21_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017730383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX9649424.1"
                     /translation="MISLSKNKVYWIFQIVGWTVYTILNILGGAANYGGNIYASKPAF
                     LVLFISEGIIFFLITHYYRNLITKRKWLNDGKIRIFYKVIVSSIIMGLLIYLFRVVVS
                     QVTSIADPKLYKLATMLGLTSINTIILFVWSLFYFVYHYFERFSASLKYEAALNEIAL
                     NQLKSQLNPHFIFNALNGIRALVEDEPAKAKKAITQLSNILRNSLVTDKKRLTNFDEE
                     LNTVKDYLGLESIRYEERLQTFYDIDPKSGRFSVPPLMLQTLVENGIKHGISTLKKGG
                     VIKLTTVVKDSRMVIRIRNSGQYLNGKSTKRKEGYGISNTLQRLNLIYGNEATFRIGN
                     EDHNTVLTEITLPLNI"
     gene            19099..19818
                     /locus_tag="VGA21_02595"
     CDS             19099..19818
                     /locus_tag="VGA21_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009185057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEX9649425.1"
                     /translation="MKALIIDDERLARKELLSLLEEHKQIEVVGEAANADEAYEMVNR
                     LNPDLLFLDIQMPGKSGFQFLESLDSVPMVIFITAYDEYALKAFEVSAIDYLLKPIQN
                     ERLAEAISKVSKFNLANDSNAKSDHKLKLTDRVFVKDGDRCWFVSLADIRLFESDGNY
                     IKVYFETFKPMIHKSLNALDERLDQRSFFRASRKHIINLSWLDSIEPWFNGGLMAKLK
                     GGEKIEVSRRQAAKFKDIMSL"
     gene            complement(19829..20200)
                     /locus_tag="VGA21_02600"
     CDS             complement(19829..20200)
                     /locus_tag="VGA21_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009581771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649426.1"
                     /translation="MDSAEKKTYLLLLSVIFHYHGLDKEEEELLNEKAELIHGREELK
                     WVDEFISKDYIHAFENAREYLKIEFPKIKKSNQIKYMEETWRANLSKGFITEMEARAM
                     VKLARDWGIEKELLEYARKTT"
     gene            complement(20245..20997)
                     /locus_tag="VGA21_02605"
     CDS             complement(20245..20997)
                     /locus_tag="VGA21_02605"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019949246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEX9649427.1"
                     /translation="MKIEFNFNGEKYAADLSNPLDISIPLREGESNPNCYWAEPPVFE
                     TIVSGDFIGSVSEGGPVNYQKISITPHGNGTHTECYGHISADGATINGTLKKFHFVSE
                     LVSIEPILMGDDSVITWESLEAKISNTNIEGLIVRSLPNSVGKLARRYSGTNPPYFDP
                     KALKMLANRNFLHLMVDLPSVDREVDGGKLLAHRNFWNFPAATRGNATITELIFVPDS
                     VPDGLYLVNLQIISLETDACPSKPLLFPMSKI"
     gene            complement(20987..22135)
                     /gene="hemW"
                     /locus_tag="VGA21_02610"
     CDS             complement(20987..22135)
                     /gene="hemW"
                     /locus_tag="VGA21_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017730380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HEX9649428.1"
                     /translation="MAKFLAKVVGIYFHIPFCKQACYYCDFHFSTDNSIMVEMVECML
                     AEITLQNSYLASDLKTIYFGGGTPSLCSPKEIEKILNAVYSNYNVDEQAEITIECNPD
                     DLNDSYLNELKLLGINRLSIGIQSFDDKELRLMNRAHDRSQALISLQLAREAGFNNIS
                     VDLIFGIPGGSLNMIRNNLNKLIEIRPEHISTYGMTIEPRTVFGNWVKKGKLVEEGED
                     QSAIQYEVIMATLKDHGYEHYEISNFALPGYESRHNSNYWKGATYLGIGPGAHSYNGE
                     SRQFNIRNNRKYIASIRKGIIPMEREVLSRSQKINETIMTSIRTKWGCDLVSLKTKFD
                     YDLYSIHKKYLEQILAEDYIIVSEGMLVLTDKGKLIADHITEKLFHED"
     gene            22177..23214
                     /locus_tag="VGA21_02615"
     CDS             22177..23214
                     /locus_tag="VGA21_02615"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HEX9649429.1"
                     /translation="MPKDLSITNFILPFLWSAIISLYAVPSVVELAWKKGLMDSFEKR
                     KIHHLLTPRLGGVAIFAGTFSGILIFTRIDNFLQYFLVSILLLFFLGVKDDLNEIKTW
                     KKLIVQILATSLLIYESEFMLTSFKGLLGFDYFYLPVQYALTIIYIVAVTNAINFIDG
                     LDGLAASIGVIISLILGFYLLRFNSMLAIACICLGGSLLGFLRYNIKESAVFMGDSGS
                     LITGFMVAVLSLSLMETTKDASGIVIAFSLLMLPVTDLVRVVFLRIFQRTSPFTPGRD
                     HIHHILIDSGFTKRKTLLIILSITSFSSLTGIAMVSIFHFDPNRAIFVLFVFSILITI
                     YCEFKRRSLFR"
     gene            23228..24307
                     /locus_tag="VGA21_02620"
     CDS             23228..24307
                     /locus_tag="VGA21_02620"
                     /inference="COORDINATES: protein motif:HMM:NF025459.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4271 domain-containing protein"
                     /protein_id="HEX9649430.1"
                     /translation="MLITFSGYAQQSDSTRIQSPILDLSYGWMENKNGKFELIPFNKK
                     SNAIHFYIRRNILAEGEFLMQIPEGSSLWIDNRLFEFFENDTTFIFSTRQFLAGNSER
                     LISIYGKNLNRTNLQTKLIKTTFRQMREISPRPRKSDQKFLLISFLSGLVLTAIFNMN
                     THGTLQGYFSINRTFNLTNREELIFKGKLFSAPNPIFYLVVSFYSSFMVVALLLLTPD
                     TFSINFRINTFTSGQLLVFQSIVMGGFYIWIVVKWMLVRYIGGLFRLTEFSTIHFFNY
                     ERFSFLFLLFMASCLVACFFAFGNETFIYSFMVSLIVLAATFRILLMLLKLLKGSPYK
                     FLHLFSYLCATEIMPYLFAIKLILI"
     gene            24344..25135
                     /locus_tag="VGA21_02625"
     CDS             24344..25135
                     /locus_tag="VGA21_02625"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009186000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HEX9649431.1"
                     /translation="MVKRSEGLFPVERLFAVKSILVSQPKPQDVNSPYFALAKKYTLK
                     VDFRPFIQVEPVTINDFRKQKIDILEHSAIIFTSRNAVDHFFNMCTQMKIDVPSDMKY
                     FCISEQTSNYLQKYIVIRKRKIFTGDKTAMDLIEILKKHKNERYLFPCSNIRKDDIPG
                     YMKEQGYNFREAILYKTVASDLSDLAEIKYDVIAFFSPSGINSLFVNFPKFKQNNTRI
                     AAFGPATAEAVNKAGLILDIKAPLPVAPSMTGALELYIKKANKLG"
     gene            25191..26150
                     /locus_tag="VGA21_02630"
     CDS             25191..26150
                     /locus_tag="VGA21_02630"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HEX9649432.1"
                     /translation="MRAHSLAVFILISFGFGLEVLGQQDPQFSQYMFNSLHYNPAYAG
                     IEGVARITAVHRTQWLGFSTSFDGNGGNPNTEILNFSLPIRKLRGGAGVELSNDNLGP
                     QNNFKALATYAFHYPIKEATISIGIRAGIFAQTIDYDQFRPVQPDDPLLVNKSGKTSQ
                     VKPDLAAGIWYSAPKYYLGVALNHLLQSQFDFGVAGQTSPLEPNLTITAGYISKAGYN
                     FTLTPSVLVKTDLNSYSFDLGLVGTFNQKMWVGGAFRQSEAAILLLGYSFLKDRALKV
                     GYSIDLIIKDREAKQPTSHELMVSYLLPFSNEPRKAIKTPRFN"
     gene            26356..27408
                     /locus_tag="VGA21_02635"
     CDS             26356..27408
                     /locus_tag="VGA21_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017730374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HEX9649433.1"
                     /translation="MENHFSIRKTLYFLAVIGCLSWITGCGLLGIGGGGRGDRGELVG
                     VPDREGWVMTIPFGMVPIPAGTFHMGQADEDVAHSQINFNKQITIGSFYMDDTEITNN
                     EYRQFMNEIIEDSLSVLGEDFVMRELYPDTTVWVKDFTHHMGDPMMAYYYWHPAFDEY
                     PVSGVDWEAAKYFCNWRTQYLNDYRQSVGDFPMPNFRLPSEAEWEYAARGGRDMAKYP
                     WGNPYIRNSKGCMLANFKPGRGNYYDDGFAYTSPVMSYFANDYGLYDMAGNVSEWAED
                     AFNPASVPLVWDLNPTYFDVNEPKKVIRGGSWKDVAFFLETGTRTFEYKDSTRAYIGF
                     RCAMTHLGRSSGREFQ"
     gene            27470..28321
                     /gene="gldL"
                     /locus_tag="VGA21_02640"
     CDS             27470..28321
                     /gene="gldL"
                     /locus_tag="VGA21_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009194427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldL"
                     /protein_id="HEX9649434.1"
                     /translation="MSSKKGGFVELLFVTIMPKVYGIGAAVVIVGAMFKILHLPGAAA
                     MLGIGLTTEAIIFFLSAFEPKHSELDWTKVYPELAEDYQGGAGAPRIANKKDSGDSVT
                     KKLDHMLESAKIGPELIDSLGKGMSNLSKNVGQMASLSNAAVATEEYSKNVGNASKAL
                     VDMSKSYATTASAMSQMANASQDAKEYHSQVQNITKNLGALNAVYEMELNDANSHIKA
                     MNKFYSNVAAVMESMEKAGKGSERFSEEMQKLTSNLTSLNNIYGNMLTAMRSGGGQSS
                     AAPKAQG"
     gene            28376..29950
                     /gene="gldM"
                     /locus_tag="VGA21_02645"
     CDS             28376..29950
                     /gene="gldM"
                     /locus_tag="VGA21_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017730372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldM"
                     /protein_id="HEX9649435.1"
                     /translation="MAGGKETPRQKMIGMMYLVLTALLALQISNSVLEKFIFIDQTLQ
                     KSVAENVGKNNKTIGRIEAEVKKKGDREGDVKALEKAKQVRQMTAALLSDLQEYRDEM
                     ARITGGYDDNGNLIGSKDYDKVGTWMIHGGKGKELKDRLNKYSTDLGSVTGESFLPLA
                     RDAKEIDLAKNDPDQKDKNFSEYYFENTPTAAGMATISQMETEVLSYEQLALDKIAEE
                     VGAKDVSFDQIRPMVLPVSNIVAAGAKFDGDLFIAASSSAVDPEMTFEGKELEVVDGR
                     GRISFVATPGAYDKDGYAKKTFKATITLSDSTYDVEHEYIVAKPVIQIQSASISSLYL
                     NCGNELNVQVPALGSAYNPSFSTTGAENVKGAQKGIVTVIPTARKVTLTVRSDGNLIG
                     SQDFGVRPIPKPILEISERGRPINLKTGVSAPGPRQINIRAVPDETFLAQLPKDARYR
                     VSEWEITLAAGARAVHSERVRAEQFAMPQTILSRVQPGMRIVIEVKRLQRQNFQNKVE
                     DVSLAGLSNIFTININ"
     gene            29953..30825
                     /gene="gldN"
                     /locus_tag="VGA21_02650"
     CDS             29953..30825
                     /gene="gldN"
                     /locus_tag="VGA21_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013454680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldN"
                     /protein_id="HEX9649436.1"
                     /translation="MKNVKLILGWVLIMILVTPAAYSQETVSEYDPNSVKQIPKYEQF
                     YKKRVWRRMDLKEKQNQAFFAFNNEITKIIIEAVKAGTLQPYKNDSLKTMMTLEEFNE
                     NMKLPGIDDEEDDIFGGDTGGGWGDDAGGGWGDTGEGEGAVEEEVVAVSTEFLPNEVT
                     LMTLCEDQIFDKRRSTLYFDIMAITITIPGDRYETGINKDVGTFKYSDLVRLFKSMPE
                     EAIWFNRQNSAEHRNLSDAFELRLFSARVIKVDNPRDQYISDVYSRYPKEHIMASQWL
                     ELELMEREHNLWEY"
     gene            complement(30822..32660)
                     /gene="uvrC"
                     /locus_tag="VGA21_02655"
     CDS             complement(30822..32660)
                     /gene="uvrC"
                     /locus_tag="VGA21_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014771328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEX9649437.1"
                     /translation="MKVNLLNAKEYALLPPDPGVYEFLNKASKVIYVGKAKNLKKRVS
                     SYFTKSLFENRKTLKLVSEARFIEFTIVNSEFDALLLENNLIKQNQPKYNIQLRDDKS
                     FPYILITNERFPRIFSTRRVIKGSGNYYGPYSSVVAMNNVLDLIRSLYTIRTCRLDLS
                     ERNIKAKKFKVCLEYHIGNCQGPCEGLQMEENYAKDMEQATAILKGNIGLVEKHFKEE
                     MKRYAVSLEFEKAQMLKEKLSLLTKFQSKSLVVNRKISDADVFTIISDENYAYLNYLR
                     IKNGAIISSQTREVKIPLDENESDILTSLVFDIQKTFSYPSTTIISNRPLTVLPDAYK
                     NFVPLRGDKRKLVELSLKNAFQYKRERQRQYEKESKDPETLIRLMNDLRLSEVPKHIE
                     CFDNSNFQGTTPVAAMVCFREGKPSKKEYRHFNIKTVQGPDDFASMREVVTRRYRKVK
                     NTGETFPNLIIIDGGKGQLNAAFEALRDLGLDSNIHLIGIAKRLEEIYTIGDQFPLHL
                     SKSSGSLKLIQQIRDETHRFAITFHRSKRSKQLTRSELENIEGIGPQTISILLHRFKS
                     IKKLNEAAPLEIEKAVGKARAQRILEYYQKKRKSKSSLKSRISKYS"
     gene            complement(32677..34974)
                     /locus_tag="VGA21_02660"
     CDS             complement(32677..34974)
                     /locus_tag="VGA21_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010611530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEX9649438.1"
                     /translation="MKATNQNSVSEKNIWRRIIFAAWTIAFLLLVVVPFYFYSVAIDL
                     GGFYGGMPGLQALENPENDLSSELLTSDGVSLGKYFRYNRSQVTYEQLSQNLINALIA
                     KEDIRFENHSGVDLWAFLRAVAFLGSRGGGSTITQQLAKKIYNTMGEEMEGSVRKFGY
                     YPRRIVAKSKEWLIAIQLEKTFTKEEILALYLNTVPFGSLAFGIKAASETFFNKTPDS
                     LSINEAALLIGLLESPSRNNPRYHPEMAKARRNVVLVQMVKYGYISQEQFDTLSVQDI
                     VLDYRVASHKEGLAPYFRNVILFDLLDFCRERGIDLYESGLKIYTTIDSRLQLYAEEA
                     VTEHMDTLQRIFNEHWEGKNPWIDRDGREIKGFIQDVAKRTERYRRLVEKYGEGNDSI
                     RIIMNTPREMTVFSYRGDIDTLLSPLDSIRYYKRFLHAGFMAMDPFTGHIKAWVGGIN
                     YNYFQYDHVRQGKNQPGSTFKPIVYTSAIENGYYPCYQAEDVPKTYVIPGNKEPYRPK
                     NAENKWSGQTLTLRNGMAQSKNSITVHITNEIGAESVVNAAHRLGITGNMEPVLSIGL
                     GTHDVSVYELVGAYATFVNRGTWTKPFYISRIEDKYGNVLQNFVPTRREAISEETAYL
                     MLHMLKGTIQEPGGTGRRLDYKYHLLDDGNEIGAKTGTTQNYSDGWFMGVTKDLAAGA
                     WVGGDDRSIHFRNIEYGQGAVMALPIWALFMQKVYSNQDLPYTKGPFPRPTFSLNVEL
                     DCSKYGAPGQRDSIDFQKPLEDIRD"
     gene            complement(34961..37531)
                     /locus_tag="VGA21_02665"
     CDS             complement(34961..37531)
                     /locus_tag="VGA21_02665"
                     /inference="COORDINATES: protein motif:HMM:NF024579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9649439.1"
                     /translation="MRIKKNTIITALIIVAAMYSSGCSSEKNTLLSISYHNLTAHYNA
                     YYYARNGIRSVESEILKNHKDDYDHFLPVYPPLDTTFTKQFEETLNDVVKKTSLAIVN
                     HGNSKWVDDSYDLLGLARLYTGEFESAIETFKWVNKNSKDGNMRHLSLIHLCRTFVEY
                     GELNNAEAVSDFLEKEDLNKRNLKKLYLTRAYLYQELEDLDKMVYNLSLVVPLLKKKE
                     GSAKIYFIIGQIYQHLGFQGEAFNNYRKCLANNPTYELSFFAKLNMAQVTELSNTSDV
                     KRVRKYFKKLVTDAKNIEFQDKIYYEMGEFELKQGNVDLAFENYTLSVQNSISNQRQK
                     GLSYLRMGQVNYDSLRNFEKAQSYYDSTIQVMPNTFEYYEEVVKRKDVLDRFVTKLKT
                     IQLQDSLLALSRLDTAELSATFINQVKSRIENEKKEIASKKTDAGDNTVTNTFQTQGI
                     NTSSLTWYFTNPSAISLGQNEFRKTWGGRPLEDDWRRSSKSAAIVSRSNPGSSISAEE
                     ESKPTVVSEDQLIASEVASMIKNVPFSKEDKESALAKIEEAYYQLGKIYHLELHEADN
                     AITNYRTFLSRFPESEFEPEVLYTLYLILKEKEDPQYISYENQLKQEHPNSTYTRLIV
                     NPNYALESSVELGELRQLYAKAYEQFDNGNFGSARILISEALASYDENEFYASFRLLD
                     ILITGKSEDLALYKNRLRAFIENYPDSDVTTFAKTLLEKAENFEQGIERAMQARFIMF
                     TQDEHYFVILVNTNDEGILTKLTVKVDSVNREIFQDQNLKTGILDFEEGWRMIVVSEL
                     NSKSTAVDFYDKILPQINLPAELRTVKFHNFIITKDNFDILYNSKDLDAYLKFFNESY
                     "
     gene            37625..38491
                     /locus_tag="VGA21_02670"
     CDS             37625..38491
                     /locus_tag="VGA21_02670"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013454684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX9649440.1"
                     /translation="MKPKKTFSNWLFNRYFLIIRNEEDFAEKRSVSFTYAKVIVLGFS
                     FLVVVMILSFFLVETLLAQWFDPRHAQMEANRQLIRLTLELDSLESEVQKKDLFIHNF
                     QNIVAGRINNEGADGDTENNNIDELQAKIDEMDYQAIDSQFRKQFETNEVSLLTYNSG
                     ERAELQELFLFSPIDGIISQKYNVRNEHYGVDVVAKKNEPVKCVADGTVILASWTQDS
                     GYIIAVQHRGNLISVYKHNDVLLEKVGNFVSAGEIISISGNTGELTDGTHLHFELWYN
                     GNPVNPEEFVTF"
     gene            38502..38933
                     /locus_tag="VGA21_02675"
     CDS             38502..38933
                     /locus_tag="VGA21_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009581786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEX9649441.1"
                     /translation="MFNKDEKKVAEEISNSSNIIGKGTILEGNIETYGNIRIEGKVIG
                     NVKTKSKIALGQSCKVEGNILAQNAEVAGEVAGRIEVGEQLLLRPSAVINGDIITNKL
                     IVESGATFNGGCKMGVKIKEIRIGENGQSATIPSKEAYKAV"
     gene            38941..39126
                     /locus_tag="VGA21_02680"
     CDS             38941..39126
                     /locus_tag="VGA21_02680"
                     /inference="COORDINATES: protein motif:HMM:NF021073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HEX9649442.1"
                     /translation="MKYSGLAFQMMATAGIATWIGYKIDQYLELRFPAFTISLLLVSL
                     IGVIYWLIKSVTNNSEK"
     gene            39123..39527
                     /locus_tag="VGA21_02685"
     CDS             39123..39527
                     /locus_tag="VGA21_02685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649443.1"
                     /translation="MRKSFLSFLINHTILTLLLAVLFIAIKIMFPGFPIPDSIFIIFV
                     FFFVFVLMVHGLLLRVAEKKAQVFIRVFMVMTFVKLFVYLGFISILILLYKSDAKGIV
                     IVFGALYFVNLAHEITAILRHLDQKKSEQREV"
     gene            39621..40691
                     /gene="atpB"
                     /locus_tag="VGA21_02690"
     CDS             39621..40691
                     /gene="atpB"
                     /locus_tag="VGA21_02690"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015264456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEX9649444.1"
                     /translation="MTERILTLKKARKYLLFSLLICSFLLANSTAFAQEEHAESESFD
                     AGKMIMEHILDSHEWHVFTFGHFHGTVPLPVILYSPTNGLAIFMSSKFHHGEETYAGY
                     KYEEGKIESLDGNVFYDISITKNVVAMLFSMALMCWVLISVANTYKRSPNTAPKGVQS
                     LLEPIIIFVKDEIAKPSIGEERYQRFLPFLLTVFFFIWLNNMLGLVPIIPGGANLTGN
                     IAVTMVLAMFTFVITTFSTNKGYWVHIFNTPGVPWWLKFPIPIMPLVEFMTVFTRPSV
                     LLIRLFANITAGHIIALVFVSMIFIFGEINIFLGYGVGVFSVLFNVAMTFLELLVGFI
                     QAYVFTLLSALYFGMAKVEEHH"
     gene            40718..40963
                     /gene="atpE"
                     /locus_tag="VGA21_02695"
     CDS             40718..40963
                     /gene="atpE"
                     /locus_tag="VGA21_02695"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020528973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HEX9649445.1"
                     /translation="MSLFILLDAAIGYMGAGIGAGLAAIGAGIGIGRIGGSALESIAR
                     QPEAIGDIRANMIVTAGLIEGVAIFAVIVSVLIIFLT"
     gene            41038..41532
                     /gene="atpF"
                     /locus_tag="VGA21_02700"
     CDS             41038..41532
                     /gene="atpF"
                     /locus_tag="VGA21_02700"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007653343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEX9649446.1"
                     /translation="MELITPDLGLVVWMTISFSLVLFILTKFAWKPIVQTLKIREEAI
                     SEALKSADLAKEEMSKLKADNEKLLEAARLERDKILKEAIAAGNKLKDEAKEDSQRIG
                     KKMIEDARVTIQNEKKTAMEDVKKQLSQFSMEIAEKILRRELENKKAQQKLVNEYIKD
                     MKVN"
     gene            41534..42067
                     /gene="atpH"
                     /locus_tag="VGA21_02705"
     CDS             41534..42067
                     /gene="atpH"
                     /locus_tag="VGA21_02705"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013454691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HEX9649447.1"
                     /translation="MSARAASRYAKSLLELARDKGKLDEVHQDMLLFNKVCDENFAFV
                     LMLRNPIINHPKKRAVLHGLFKSKTDPLTMAILDITTRKNREKLLPMIAKEFHYQYNI
                     ERGIQEASITTTFELDDSLRKKFNEIIIEISKAKTLSLEEKIDENIIGGFLLKVGDKQ
                     VDESISNKLKQLRLKLS"
     gene            42100..43680
                     /gene="atpA"
                     /locus_tag="VGA21_02710"
     CDS             42100..43680
                     /gene="atpA"
                     /locus_tag="VGA21_02710"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015264452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEX9649448.1"
                     /translation="MADIRPDEVSAILREQLSNFRSVAELEEVGTVLTVGDGVARIYG
                     LSKVQSGELLEFENGLQALALNLEEDNVGAVILGDSRSIKEGDTVKRTKKIASIRVGD
                     KMCGRVVDTLGKPIDGKGAIDGELFEMTLERKAPGVIYREPVSEPLQTGIKAIDSMIP
                     IGRGQRELIIGDRQTGKTAVAIDTIINQKEFYEKGETVYCIYVAIGQKASTVASVVSS
                     LQRHGAMDYTIIVSATASDPAPMQFFAPFAGAAIGEYFRDIGKPALVIYDDLSKQAVA
                     YREVSLLLRRPPGREAYPGDVFYLHSRLLERAAKINRSDAIAKQMNDLPASILDKVKG
                     GGSLTALPIIETQAGDVSAYIPTNVISITDGQIFLETDLFNSGIRPAINVGISVSRVG
                     GNAQIKSMKKVAGTLKLDQAQFRELEAFAKFGSDLDKATQLTIDRGRRNLEVLKQGQY
                     APVPVERQVAIIYASTKGLIDKVPINRVREFEAEFLTILEAQHRQALDTIKAGKLDDD
                     VLETLGKVAKELVPKFAK"
     gene            43697..44560
                     /gene="atpG"
                     /locus_tag="VGA21_02715"
     CDS             43697..44560
                     /gene="atpG"
                     /locus_tag="VGA21_02715"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009581793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HEX9649449.1"
                     /translation="MANLKEVKNRISSVISTQQITKAMKMVAAAKLRKAQDKIIQMRP
                     YAQKLNSILQNVASGNLNVDDNDFVQVREERSVLLIVITSDRGLCGAFNSNVSKAALR
                     RMNYLEEQNGSTVTILPLGKKANDFFTKRKAKKVVDFLSIFSELNFESARKVADFVMN
                     DFRKKEFDRVEIIYNQFKNVATQILTTEQMLPIQQVGGEGGRTLDYIFEPSKEYIVRE
                     LIPTSLKIQLYRALLESNAAEHGARMTAMDKATENAGDLLDNLRLDYNRTRQAAITKE
                     LSEIVAGADAL"
     gene            44615..45448
                     /locus_tag="VGA21_02720"
     CDS             44615..45448
                     /locus_tag="VGA21_02720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HEX9649450.1"
                     /translation="MKAIARTFNSLSLIIVAGACISSIFISRVLESELSLLSIISLGI
                     SVWIIYTFDHYIDAKQLNNIKGKPRHQFHLENLALIKFLLIAAVCVNTVILIFLPNEV
                     LIMGIILLLFITGYFMIIKLPIQYIGVFKELIIAAIYSAGIFIPAIATHSKTVSISII
                     PLYLVFFLLAWINLLLFSWFDFEYDRRAGFASLVIVLGRDFSNKIIWILLIIFYILWI
                     WTFYFFTSGSFPGELKWITIAMAIILSAMQNNKRLFYKGENYRLVGDAIFLLPLMALL
                     K"
     gene            45445..46071
                     /locus_tag="VGA21_02725"
     CDS             45445..46071
                     /locus_tag="VGA21_02725"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX9649451.1"
                     /translation="MNSYADFSGIAFAYDYLARIFFGDTIKRCQIWTLKSLQENATVL
                     IPGGGTGWILEEIDKLNIPLEIVYVEPSKGMMRRSKERKTNNLKIHYINEMNGSSSTE
                     FDKILHFFYLDLFNEVNLEAEIISARNLLKPKGFLLFADFQLNRKTLPVWQKSLSFIM
                     HQFFRRVTGMESNKLKDLHSAILKSGFNLVEEKFFYGNFISGKIYQKI"
     gene            complement(46068..47423)
                     /locus_tag="VGA21_02730"
     CDS             complement(46068..47423)
                     /locus_tag="VGA21_02730"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX9649452.1"
                     /translation="MSRSAALIFFILIISYSAASAQSIDLRRGLMAHYSFDNDFSDQS
                     EFGSHGTSYGATLDPEFRCGNGVAKFNGLDDYIDFGNPDQLNMPYSGLTVSFLFYPTK
                     LNPEDFQLIVGKWGFDAKKDQFAVFINVQNLLTFAVADGKEFGYGVYSKTIFEPFQWY
                     HIILVWNRSRKVGILVNGEMDKTGEQTGSGYNSNSEITLKAGRQVVRQNRPFEGYLDE
                     IRIYNRSLSLEEMKALYLRDVLPCNQFTLKGTVYNQKSRNPIGGANIVFEDLDTGSEY
                     VNISSQDQSGYYETILPIGYQFGFYAEAKDYISINDSISTARVRKNSVIEKDLYLVPI
                     EIGGTVRLNNIFFDLDKAILKDESFPELDRLLKIFEQVPGLVIEIGGHTDSQGSDEYN
                     RVLSDARANAVRTYLLEHGIGEDKVTAVGYGESEPIDTNDTEEGRAQNRRVEFKIISK
                     D"
     gene            complement(47487..47924)
                     /locus_tag="VGA21_02735"
     CDS             complement(47487..47924)
                     /locus_tag="VGA21_02735"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEX9649453.1"
                     /translation="MHNFKENAEVSIGMEKYRSKIIIRNHELIADEPFESGGQNLGPN
                     PYDFLLAALGSCTAMTLRMYADRKEWNLTGVNVKLGHEKIHAEDCKDCESTEGKIDTI
                     SRVIYLEGELTDEQRKRLLEIANKCPVHKTLHSEIKVRSSLGE"
     gene            48084..49253
                     /locus_tag="VGA21_02740"
     CDS             48084..49253
                     /locus_tag="VGA21_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008623479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649454.1"
                     /translation="MNLVEVLNPKQEKLFIQFAVQHYRNEPKWIRPLDKDIRFIFNKK
                     KNKSYRNGGESIRWLLYNNDEIIGRIAAFYNPKLAGRDNEQPTGGVGFFECIDDQNAA
                     SMLFEVSKDWLKDKGMEAMDGPVNFGDRDAWWGLLVEGFNEEPNYRCNYNYPYYQPLF
                     ENYGFQLYFRQLTYARKTADPLSPKLHIKAEMISSDPGYSFAHFRKKQYKRFAQDFMN
                     IYNQAWAGHVGVPKLTPAQAELSMKLLLPIVDEKIIWFGYYKNEPVAFFIMLPEVNQI
                     FKYVRGKLDIMGKLIFLWHKWNKTCRKMIGVVFGVVPEHQGKGVEGAIIMAARKMVQD
                     DYARYEDFEMNWIGDFNPKMIRVVEQVGAHVSKVHNTYRYLFDRNVPFKRMPIKN"
     gene            49254..49841
                     /locus_tag="VGA21_02745"
     CDS             49254..49841
                     /locus_tag="VGA21_02745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649455.1"
                     /translation="MGKWYKVSRKKRRFLLKNLGKGLLWLAAIVLLFVIIKRYVPIND
                     TSWIYKLYDKPLLVYLIFLASEIIVGIIPPEIFMIWALKSTTAKFYVVDIILLSTISY
                     FAGILGYFIGKWFHDSRFYTFLHGKYLYQYEKFLKRFGGFLVIVAAATPLPFSGICVL
                     VGSVKFNFKKFLLFASARFIRFALYGYIIWQANTI"
     gene            complement(49844..50986)
                     /locus_tag="VGA21_02750"
     CDS             complement(49844..50986)
                     /locus_tag="VGA21_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013927348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HEX9649456.1"
                     /translation="MRVYLDNASTTPLDTEVIAAMEPFLKTHFGNPSSIHSHGRESRS
                     AIEKARKVIAELLNVSPSEIFFTSGGTEADNSVICGIVHSLGVKNVISSRIEHHAVLH
                     TLDHISSQGLANIHYVNLDEKGHIDYGHLAELLNQNPNSLVTLMHANNEIGNISDLEY
                     IGNLCKESDSYFHSDTVQTVGHYVHNLQLLNLHSMVGSAHKFHGPKGIGFMYIRGGLK
                     IDPYIHGGSQERNMRGGTENVYGIVGLAKALEICYRDMNKHRSHIENLKTLLISKLDG
                     QLDGVSYNGDSANLEKSNYKVLNVCLPESPENEMLLFNLDIGGVSASGGSACTSGSNL
                     GSHVLKAIGADPERGAVRFSFSKLNTEDEILFAAERLSELYVTSQE"
     gene            complement(50990..52399)
                     /gene="glmM"
                     /locus_tag="VGA21_02755"
     CDS             complement(50990..52399)
                     /gene="glmM"
                     /locus_tag="VGA21_02755"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009583285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEX9649457.1"
                     /translation="MPENVIVALISSISGIRGTIGGKPGEGLSPVDVVNFTSAYGTWI
                     NKTEQNKIVTLGRDARPSGLMVKNLVSSTLTSMGISVIDLDFATTPTVEMAVTGSNAG
                     GGIIITASHNPENWNALKLLNSKGQFLSPAEAAEIFSIAESGNFEYSQVNQLGSISRS
                     EEWLNYHINKILELKLVSPEKIKALAPRIIVDVVNSVGSIAVPALLQRLGITEFKILN
                     GEMNGHFTHNPEPLPENLGELSSEVVKGNFDLGIAVDPDVDRLSFVCEDGTFFGEEYT
                     LVSIADYWLSHYPGNTVSNLSSSRALRDITLKHGGKYSAAAVGEINVVEEMLKTNAVV
                     GGEGNGGVIIPDLHYGRDALAGIALFLSQMAASGKKVSEIRSQYPDYIISKNKILLDP
                     SIDVDEVLSQFHILHKNEDINTLDGIKVDFADYWIHLRRSNTEPIIRIYSEAKSAETA
                     RKLAATTINQIENIIKAKV"
     gene            52632..52862
                     /locus_tag="VGA21_02760"
     CDS             52632..52862
                     /locus_tag="VGA21_02760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649458.1"
                     /translation="MSVLSVPLTPDLEQAINNLLKWGVASNRADAARKAILKLEEDTA
                     IQVVLESEQAALEGKLISGNLRGFIKKSSTKK"
     gene            complement(53017..53217)
                     /locus_tag="VGA21_02765"
     CDS             complement(53017..53217)
                     /locus_tag="VGA21_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017730345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649459.1"
                     /translation="MTTLLLAVGIVALFFILMSVRLIFLKNGEFKGTCASQSPFLNKE
                     GVTCSYCGKKVNEGEACAKEAS"
     gene            53305..54093
                     /gene="mazG"
                     /locus_tag="VGA21_02770"
     CDS             53305..54093
                     /gene="mazG"
                     /locus_tag="VGA21_02770"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009194032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HEX9649460.1"
                     /translation="MGKINKTTDNRRQEKLKAFDRLLTIMDELRENCPWDRKQTNESL
                     RHLTIEETYELSGSILENDYNEIKNELGDLMLHLVFYARIASEKNEFDIGDVLNSVCD
                     KLIKRHPHIYGDVEAKDEKTVKENWEKIKLKEGNSVLGGVPKSLPAMIKAMRIQEKAR
                     GVGFDWQDKKQVWEKVMEELGEFLKEKNKERKFREFGDLLFSLINYSRFEDINPEESL
                     ERTNQKFIKRFSYIEDRIREKGKNISEATLSEMDKYWNESKKFN"
     gene            54268..55812
                     /locus_tag="VGA21_02775"
     CDS             54268..55812
                     /locus_tag="VGA21_02775"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009583290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HEX9649461.1"
                     /translation="MNASEDNLLKKVIAHSKEYGFIFPSSEIYDGLGAVYDYGQNGAL
                     LKNNIKEYWWRAMVQLHENIVGIDSAIFMHPTVWKASGHVEAFNDPLIDNKDSKKRYK
                     ADNLVEDHIEKIRGKISKEIEKAEKRFGAEFNKEMFLETNPRVKEYSEKIKTIEDRLR
                     GALGSGDLAGLKKLIDDLEIKDPVSGSGNWTEVRQFNLMFATDMGSVAGESNKIYLRP
                     ETAQGIFVNFLNVQKSGRMKIPFGIAQIGKAFRNEIVARQFIFRMREFEQMEMQFFVK
                     PGEEMKWYEFWKKKRIQWHKNMGISQEKYRFHNHINLAHYANAACDIEFEFPMGFKEL
                     EGIHSRTDFDLKAHEEHSGKKLQYFDQEENRSYVPFVIETSIGLDRMFLAILSSSYKE
                     EKLEDGSERVVMNIPMFLSPVQVAILPLVKKDGLPEIATEIMNELKYSFQCTYEEKDT
                     IGRRYRRQDAIGTPLCVTIDQQTKQDQTVTIRFRDSMEQKRIKIGELNSVISELVSLK
                     SFLRKL"
     gene            56050..56952
                     /locus_tag="VGA21_02780"
     CDS             56050..56952
                     /locus_tag="VGA21_02780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649462.1"
                     /translation="MKESYSPRAALPLVFCTFIFSCSVLQENRINEELLDNFPVPDGR
                     SYGLVISYEVESLSFSEFSGGDPPQELALQSEPAVSRSKIKTVIYQDGSSDWLIKNVK
                     PRNPPVEFHNIPKSDYDEISTIRIINNQGYFINGNGDLIHKQRIDVPSFAELILDVQN
                     SNNKNINARVSEVLSGTIFLSEQRLSELREINLVEELPSGLIKIKDLELYNKNFNSRT
                     QEEELIYETYGDPITGLVVGGKVLNQNGELISYVVYKYSEAGMGSILEMIHYKEFHSG
                     DDEGTEVGILNNYFENIVIENHLN"
     gene            56959..58182
                     /locus_tag="VGA21_02785"
     CDS             56959..58182
                     /locus_tag="VGA21_02785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649463.1"
                     /translation="MKILRIILCFAITFLANQISQSQDRPVIWVHGLNDNNTFWANMA
                     TVFNTERRMTSSNLNYGTGNGVVNFATQIRNNNIARTSNIVIGHSMGGVALREVNRVN
                     SNFFGGAITFGSPLDGARIVNSWANGSFGSFIQKSTKELTAGPLAEYGVDVKLLDYLG
                     RKWLGATLSLIPRDFIMNQFNNLMTGQSAADLAENSPYYSGVKNFYSGIPKINVFGNE
                     NAPAMQRLASISLSNGQNDTDVIGLISSAKSVYTAFININNTRALLNPLHAPIYYARS
                     YQWKRGLDFFNKSLEPGWAGLIGSTRTETTTYTYETYVCDEDEFNYNNQLERIETDCW
                     QTVTKTYTKTIIEPSDGLIHKSSQIGAKSSWKGTAVEALGVNHLEMGRHWESERILRQ
                     IFDGSIHPNISIPRR"
     gene            58179..>58715
                     /locus_tag="VGA21_02790"
     CDS             58179..>58715
                     /locus_tag="VGA21_02790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649464.1"
                     /translation="MNNNFYLRLLFLTAILISCRDHFEIEIDQNGAVLKTSHLWTKHL
                     VNEGDELFTFITPFELGETVTLMGADFKEQALRKLDINNGNQIWEWNEFNFEDRWDVY
                     YYHSFDNKLIVPNGANFYSIDIKTGQTIWKEKLPYQVNRSVAGYRENYFFSGYLYEIE
                     TRPTIFKGNLYTGEYAPFL"
BASE COUNT        18205 a        11160 c        11941 g        17409 t
ORIGIN      
        1 caaattaccg aaagcgctct gacatcaccg caaaagtgcg attgtatttc gctcctatga
       61 atttaacttt tattactcac ctattcctgg attttcaaaa aaatataaag actcctttca
      121 acaaattgaa attaagcaat aaatttagat agtagaaata cactgtacct ctctactcca
      181 tattcccccc gcccttgttg gtgttcttca ccaacaagcg ttgtgctaca caaatcacgg
      241 cattagcagc atccgactct gagttaattc cggtttattc aaagatcaaa atgctaaatg
      301 cggtattgca taatacctga gatggcagta agtaattaca ggcttgcttg taaagaatat
      361 tctttcccaa ttagctctgg ctacctcgta tagcgattta ttacgcttgt tggtgaagaa
      421 caccaacaag gtctagggtt gtttataatt tgtcatcgca acacaagttt aattaaactt
      481 ggtcaactga agggaggaga aatcttccct tctttcaacc tcttgtgttg cacttattag
      541 ttgaacttag aattaaggaa tatccctaat taccctaatc ccgattacct catttacccc
      601 acagggaatt aaggaattaa ggaattaagg aattaaggaa ttaaggaatt aaggaattaa
      661 ggaattaagg aattaaggaa ttaaggaatt aaggaatatc ctaatcccta attaccctaa
      721 tccccaatta ctcatttacc cccactccct aattacccta atccccgatt acctcattta
      781 cccaacaggg aattaaggaa ttaagtgatt gagtgattga ggaattaagg aactggggaa
      841 acccgttcat ccctaattac cctaatcccc aattacccta atccctaatt accctaatcc
      901 ctaattacac taatccctaa taaccctaat ccctaattac ctcatttacc caacagggaa
      961 ttaagtgatt aagtgattgg ggaattaagg aactggggaa acccgttcat ccctaattac
     1021 ctaatccccg attacctaat ccccaattac ctaatcccta attacccaat ccctaaaaac
     1081 cctaatcccc aattacccta atccctaaaa accctaatcc ccaattacct catttaccca
     1141 tttaccccat ttaccccatt taccccattt acccaacaag gaattaagtg attggggaaa
     1201 cggtttatgc aagatatttt tgaactttac agacaaaaaa gaaagatgca gttcaaaagt
     1261 attaatccat acaataacgg tgtattgcag gcctttgatc tgtttcctga aacccgatgg
     1321 aaaaaatccc tggaaatatc agaagaagtc tacaagagtt ggcgaaaaac ttcatttcag
     1381 caaagagccg atctgttttt ccggttatca aacttattga aagaaaaaag ggtggaaact
     1441 gccaaattga tgagccttga aatgggcaag gtacacaaag aggcagtagc tgagattgaa
     1501 aaatgtgcct gggtatgcga gtattatgct gaaaatgcgg agaaatttct tcattctgaa
     1561 agcattgata gcacagcaga taagagcttg gtcagatatg atcctatagg agcagtgctt
     1621 gcaatcatgc catggaactt tccattctgg caggtattca gatttgccgc cccatcttta
     1681 atggctggca acgtagcatt actcaaacac gcacctaacg ttaacatgtg ttcactcacc
     1741 atagaagatc tatttactga agcggggttc ccggcaggtg tgttccaaag tttaatagtt
     1801 gatgtggatg tagttgaaaa gataattaat cacaatattg ttcaggcagt gacacttacc
     1861 ggcagtgaaa gagcaggtgc ctctgttgct tccattgccg gaagcaatat taaaaaaagt
     1921 gtattggaac tcggcggctc cgatccattc atagtgctgg cagatgcaaa tcttgacaac
     1981 gctgcaagaa ccggtgccac atcaagaatg ctaaatgcag ggcagagttg tattgccgcg
     2041 aagagaatga tcgtagttga atccgtgcac gatgaatttg ttgaaaaatt cagaaatgaa
     2101 atcaataaga taaaagttgg agatccctta gctgaaggtg ttactatggg accgatggca
     2161 agaatggatc tcgcggaaag cctggagaaa caattaaagg cttcattgga aaaaggagca
     2221 aaattggaat tgggtgggtc acggaacaat tgcatttttg aaccaacatt aattaccaat
     2281 gtaaaaccgg gcatggccgc atttgaccag gaaaccttcg gaccgttggc caccgtgatt
     2341 aaagccaggg atgaggcaga ggccgttgaa ttggccaaca agacagagta tgggctggga
     2401 gcatcacttt ggactaccga tcttaccagg gctgaacaac tgagtgctga aatcaattca
     2461 ggcagtgtct ttataaatgc catggttaaa tcggatcccc ggttgccttt cggcggcata
     2521 aaaaagtcag ggtatggccg tgaactttct catcatggca ttaaggagtt tgttaatgca
     2581 aagacagtat ttcgcgtgga ttcataaaat ttgttaaaaa atcctccgtt tggaatattg
     2641 aagataccct ttagcctgat tttcgcggtg atataaataa tattgcctta gatttacgcc
     2701 cgcgattttg gaaaaacgcg aaggaaatca tttagttaat aaaagataag aggatggaag
     2761 aagccgcaac taaatttgaa ggtataaacg aagagcagag aaaaattcat cgggaaaaaa
     2821 taagacaggt ctatgatgag gtgggcagag tagtagtagg gctcagaggc ctggttaacc
     2881 gcttgatgat aggtcttttt acgcaaagtc atatcttgct ggaaggtgtt cccggaattg
     2941 ccaaaacttt aacggtatca acgcttgcca gagttctgca tcttgatttt cagagaatcc
     3001 aatttacccc cgacttactt cctgcagacc taatcggtac catgatctat aaccagaagc
     3061 aaggaaattt tgaagtaaaa aaaggcccca tctttgcgaa tatgattctg gcagatgaaa
     3121 taaacaggtc gccggctaaa gttcagtcag cgctccttga agcgatgcag gaaaaaaatg
     3181 tcaccattgg agaaacaaca tttgaacttg acagaccctt ctttgtcctg gctactcaaa
     3241 atccggttga gcaggaagga acttatccat tacccgaagc gcagatagac agatttatga
     3301 tgaaggtaaa tgtaggttat ccaagcaaag atgaagagat ggaagtaatg cgacggatgt
     3361 ctaacatgaa atttgatgat tccgtaaatg ccgtactttc aaaggatgat attttttcaa
     3421 ttcgggatga aattaaccag gttaccattt cggagtccct ggagaaatat atcattgaac
     3481 tcgtatatgc caccaggcat cctaaggaat atggtttaac cgaagcagca aactacattc
     3541 aatttggcgc gtcaccaaga gcaagtatta atcttaacaa agcagccaaa gcgattgctt
     3601 tttttgaaga cagagattat gttttgccag aggacatcaa ggatgtggcc atggacattt
     3661 tgaaccacag gttgataatg aattacgagg ccgaagcgga tggcgtttcc tccgttgaca
     3721 ttattaccac cgtgttaaat aaaatcccca ttaaccgcta aattcagcta ttgaattaat
     3781 gttgagttaa cacttgtttt aagtgtttaa tgatttcgta ataaagcatt tcccatcaat
     3841 taacattatt gttaaatctt tgttggcaaa tagaaactaa agccaacaat gaaaaaaata
     3901 acacatcttt tactgatacc attttgtttc gcattggtat ttatttcatg cgaagaagat
     3961 aaaggaggaa ttgggggaga aaccggagca gaaaacgaaa ttctggttga agccaatatt
     4021 tcaggtaatg aagtttggtc aagcaacttt atttacatac ttgccggacg gataaccgtt
     4081 ctggatggag caacacttac catagaacca gggacggtta tcaagggaca agccggcacc
     4141 ggttcaaatg cgaccgcttt aatggtagcc aggggtggca ctttaatcgc ggatggaatc
     4201 gcgaccgctc ctataatttt tacttctata gcagatgaaa ttaatccgga acaggtagcg
     4261 gctggagatt tcaaaagccc taacctggat ccggatattg acggtctgtg gggcggtgtt
     4321 attgtactgg gaaaagcacg gatttccgca tccaactcca gtggagacgt aagtgaagtg
     4381 caaatcgaag gtataccgac cagtgatagt aatggtttat acggcggcaa tgaagataat
     4441 gataattcag gaatcctaag gtatatatcc attcgtcatg gaggtgcaaa cataggttca
     4501 ggaaacgaaa taaatggctt aactctgggt ggagttggtt ccggaactac tgttgagtac
     4561 atagaagtag tagccaacca ggatgatgga attgagtggt ttggcggaac ggtaaatact
     4621 tcaaacgttg tagtgtggaa cgtaggggat gacggaattg acactgatca atcatggtcg
     4681 ggtacgctga ataattttgt tgtgataaca cccaccggac attgctttga gttggatggc
     4741 cctgaaggct cttatgaagc cggtcataca attcaaaatg gaaccataat cgcgtcaaca
     4801 gatgatggga gcaggttttc agaacacctg ataaatcttg ataataactc catcgtttcc
     4861 ctgagtaatt tgtatttctt caacctggtt gaaggtcagg tgattaacag gattcaatgc
     4921 gtggatggga actgcccttt cagtggaatt caattactgg taccagatgg caatgtgctg
     4981 gaagattttg tatacggtgg cgtggtaccg gcgggtatta ttgaagtatc tacgccctcg
     5041 gtgggagcaa gttcagcctt gttcgcgtgg acctgggctg cacaagtggg tatgctaaat
     5101 ggcttgtaga atttataaga tacgtatcat aaataaagaa atttcaaata ataacttgat
     5161 tctcaaagct tgcttcccca ggaggcaggc ttttgatgat ttaataataa tagaaaaatt
     5221 tcaatcactt tattccaaaa ttgaatgtac agactgacca gaatcgcact tgcctttttg
     5281 tctacaacct tacccttttt cgctgccgcg cagggtatag tgcgcggaat aataattgaa
     5341 gataaaaatg gagaaggact gattggtgtt acagtagtcg ttaagggaac gacaacaggt
     5401 gccataacgg attttgacgg caactttgaa attaaattgg atccgggtga atacgattta
     5461 caagtttcat tcatttcatt tgaaacggtt acaataacgg gagtattggt gcaaacaggt
     5521 aaagtaactt tactcaatgg aatccggttg aaagaatatg tgaaagagtt ggaaggcgtt
     5581 accataaccg ccgatgttat tcgaaattca gagtcagcgt tactgacagt aaagaaaaaa
     5641 tcgccaaatc ttatcgatgg aatatcttcc gttaacttta aaaaaatagg cgattctgat
     5701 gccgcgggtg ccgtcaaaag agttaccggg gtttcagttg aaggtggtaa gtatgtttac
     5761 gtaagaggtt taggagacag gtacacaaaa acaaccttaa ataatgtaga tatacctggc
     5821 ttagatccag acagaaacag tatgcagatg gatatttttc caaccaactt gattgacaat
     5881 ataatggtaa tgaaatcctc tctagctgaa atgcctgcag atttcacagg cggcgcagta
     5941 aatattgaaa caaaggattt tccggatgaa aaaatattcg atatttcact gggaattggt
     6001 tataacccgg gaatgcacct aagacctgat tacctggtct atgagggcgg aaaaacagat
     6061 ttcctcggct tcgatgacgg aaccagatct ctgcctggag aagcgagaaa taaccctgtt
     6121 ccctcacctg ttagcggtga ttctcagcaa gatgttaacg cgtttttaaa caaatttaac
     6181 ccaaccctgg gggcaaaaag taaaaccagc tttatggact acagcctggg agtttccttc
     6241 gccaaccaaa cctccctgaa gggagataag aaactcggat atatcctttc aactacctat
     6301 aagagttcga ctaaatttta tgacgaagtc atttatggtg aataccagcg gttgataaat
     6361 ccttctttat acgaattgag gtacgcaaca atacaaaatg gatccgttgg ggaacataat
     6421 gtattgctgg gcggcctggg aggaattgct ctaaaaacca aaaactcaaa gttgaaattt
     6481 accatcatgc atcttcaaaa cggagaaagt aaagctggcc aattcttcgt ggataacgat
     6541 ggagaagcag taggtcaatc aggttatttt gcatcctcgg ataatcttga atatacccaa
     6601 cgaggactaa ctaacctgtt attgaatggg gagaaacaca gtataaatac attatggaaa
     6661 ttggattgga gcgtatcacc cactttttca aagataacaa acccggatat tcggaaaacg
     6721 gcatttacac aaacagttgt agatacccta tttagtgccg gcgccggagg taacccaaac
     6781 cgaatttggc gatacctgga agagttaaat cttgtatcaa aagccgcggt ttcaaaagat
     6841 tataaacttt ttggggaatc agcaaaacta aaatttggtg ccagccatat tttcaaaatg
     6901 cgcgattatg aaatactagg cttcgacata cagtttttcg gaactcagcc ggatttcaat
     6961 gccgatccca ataacgtttt aaacaatact aatttatttc ccaatggaac tttatactac
     7021 tcatcaggca ataatatccc aaatccaaat caatattact catcagtaca taatacgggg
     7081 atttttgtat ccaatgagtt ttcaccactc gctaatttaa aagcgattat tggattgagg
     7141 ggagaaaaat acgtacaaag acacacagga agggacgttg aatatgcaag tctgggaacc
     7201 gggaataatc tggataacga gaaagtgttg gatgcttttg atatctttcc ttcattaaac
     7261 ctgatttatt ccctcaacga tcagcaaaac ctcaggtttt cgtattcaag aacaattgcc
     7321 aggccttcat ttaaggaatt atcattcgct caaattatag accctatcac caatcgaata
     7381 ttcaatggtg gtttatttac ttatccggac tgggaaggga aactcaccga gaccagcatt
     7441 gacaactttg atttgagatg ggagttttat atcaaaaggg gagaactaat ttcggcaagt
     7501 gctttttata aaacttttga tgatcccata gaattggtta gaattccgga acaacaaacc
     7561 agcactgaat accagcctcg aaatgttggt gacggccggt tgtttggtgc tgaaatagaa
     7621 cttagaaaaa accttgattt ttttgcgcca tcattggaga atttcaacat cagcggaaat
     7681 ttcacctatg tccggtcgca aatcgatatg accaatacag aattcaattc aagaaaaaca
     7741 tatgaaaagg atggtcagga aatagaaaat gtccgggtaa tggcaggcca ggctccctac
     7801 atcattaatg caggcttttc ctataataat ccggtagtcg gtttggatgg aggttttttc
     7861 tacaacgtaa aaggggccac tctcgttatt gttggtgccg gtctcttccc ggatgttttc
     7921 gcgaagccat ttcatagttt gaactttaat atgaataaaa ctctgggctc tgaccagaga
     7981 tcaatcatta ccctgagtat ttccaattta cttaatagtt caaggaagga agtttattca
     8041 gcatttagag caaaagatca aaacttcaca agatttgagc cgggaacaac aataagcgtt
     8101 gggtggaaat actcattctg aacaggtccg gaacgaattc caaacgctgc ggataatgcc
     8161 tcacggccgt cttcagttta caagtgtcat gccaactgat tggtaacagg tttttggtgg
     8221 cagctgatca gcgatgtcac taccggaacg acctgctaaa ttgtctaaga tggagaaatt
     8281 agcattgtcg ttggaatcta cctttcaata gaaaagtaaa attgataatg tataatttgt
     8341 gaacattcag aaccacctgc gctaatacag tttctcagtt ccacaaaatc atttcccgca
     8401 aaatccgcaa tagcttgata ttcaaaaacc catttattct tatttgaaag tttgatcgta
     8461 caaatccttg aatttttagc aactttaata atcgaaaatc cgccttcaat cgggatttct
     8521 cctaaatcat actgataaat ttctttagag gtaagattaa catttatttc tactatttcc
     8581 tgaggtgaaa tatgtcttcg gtcattacaa gacgctacaa tcaataacaa aaagaatatc
     8641 gtaaccaatc cgaattttag caacttacct agcatattca attgcattta ttatttgaca
     8701 ccatattggc caaattggtt gttaagaatt tagtctgtta cgaatatatt cggatcaatt
     8761 ctactccttt ccttcaggtt caaatttata tcctacccct tttatggtag caatatattc
     8821 gtcaccgatt ttttccctga ttttcctgat atgaacatca acagttctgg aaagaacctg
     8881 ggaatccaag ccccaaactt tatcaagcaa ggcatcccgg ttaaatattt tccggggatt
     8941 ttcagcgaga aagtgcaaga gttcaaattc tttttttggc aatgaaataa tcctatcgcc
     9001 tacatgcacg gtataactca ttttgtcaat aaccaaatta ccattatcaa gaggagccgt
     9061 atgtatccgc ttaacttcac gggttaatag cgaattaatc ctgctgatta acgcccgtgg
     9121 cctgattggt tttattaagt aatcatcagc gccggtttca aatgcggcaa tttctgaata
     9181 ctcttccgtt cgggctgtca gaaataaaat gtaagtatca cttaattctt tttcctggcg
     9241 taataacctg cacgtttcaa ctccatcctg gtagggcatc atgatatcaa gtatggctaa
     9301 atccggtttg aattcaagtg ccgccgtaag tgtctccctg ctgtctatta atgatcttac
     9361 ctcaaatccc tccttttcaa gattgtattg caatatctca atgatatcgg gttcatcatc
     9421 aacaaccaga atcttgtatt tctttttcct caacggtaat tatggaattt ttctctttac
     9481 aaagaaacca gctaagtgtt attaggactt tatcagactg ttaagtttaa tttaattaaa
     9541 aaaaaggaat gtggattgct gatttaaagg caattcgata atcatttagg aacactgttt
     9601 agcccggatg tttctttctt aacattcact taacatataa ttttccagca gataattcaa
     9661 cgataatagg acacaataat tttgtttgaa catttaaagg aactttcatc ctttatagaa
     9721 attaatttca ttgggtttgt acataagcag gaaaaggtat tttttcctga attcaggact
     9781 tgaatcttag aatcttccgg gttaaattag aatactatta atgaagcaaa gaatgagtcc
     9841 cacgtctccc caatcattaa aggcgcaggt tagaaaagct gccctgagag cttacgcaga
     9901 tctgcttatt gatgatcctt ctgatattac cgatcagcat ttcaagattc ttttgaaaga
     9961 catgagtccc ggcgaaatta tttatgccac ttccaggatt ctgggaaagg aatccgatat
    10021 ggtgaatatg gtaataggaa gatttaacaa ttatatggag aaggcgggaa gtgcaattgt
    10081 taacatttaa gtaaaagctg attttttatt cccggaaatt tgttgtctcg gttggtcgaa
    10141 gttaattcaa tttcttaagt cgttctttgt gcatctccag caccttcctg atttgctcca
    10201 gttcttcgta tgttttctcc tgatctgcct tattattttc aatccgctgt ttcaaaactt
    10261 caatctccag ttccatggtc ttcacacttt cttcaagcct tcgcttttca ttctcggcat
    10321 caagcagccg caatgtcagg ttttccaaat tctgtaataa cctttgatgt tgacgactgg
    10381 taaatgctgc cgccctctca gcgttttgaa tgtcatttaa tacaagtgat ttcttataat
    10441 ccaaaacgta ggtgtacaac atcccgctaa tttcattcat tagcttgccc gattcttctt
    10501 cttcaagaac tgaagaatca attcctagcc aaatggtgac aatcgaatcc cgcgattgat
    10561 taatctgggt aacccccgtc agcaacttgt ccgtaatctg agggaataca aaatcttcta
    10621 aaaagtacaa ggaattcttt tttctaacct ttcccttatt tttcaataac tttgtccacg
    10681 catccctatg cttttcaaca ctgccattaa cagtcgtttc gtagccggca taggtcactt
    10741 tctcaatggc cttactttct tcttttactg taatttcctg ggcgaagacg aaactaaaat
    10801 tcagaaaaaa ggttattatg taagtagtaa atttcattgc attatgcttt tatgcaagct
    10861 ataaaatttc atcgtagagt aaaatcaaaa taccatcatt aacaatgaat gctcattgat
    10921 atgcctaaaa aagttttaat tgccggtggt tccggtagta tcggcactca actaaccaaa
    10981 ttattgctgg agaaaaactt ttccgtttca tggttaagcc gggaaaataa aaccacggaa
    11041 ggagtaaaaa cctacctttg gaattatgaa gaaaatatca ttgatgacaa tgctttaaac
    11101 aacactgaaa tcattgttaa cctggcaggt gcgggaattg ccgataagaa ttggagtaag
    11161 gaacgaaaga aaatactgaa ggaaagcaga attaaaacca cgaatttatt gctcaacaaa
    11221 attgaagaaa agccaaacga tgttaaaacc tacatatcag cgtcggctac gggatattac
    11281 ggctacgaca gcgggtcaat agaaaagaaa gaaggaagca ggtttggcga cgacttcctt
    11341 gcaaccttaa ctaaagaatg ggaagctgca gctgatggct tttccagcaa aggcatccga
    11401 actgtgaaat tgagaacagg ttttgttgta ggcaataatc aaaatggatg ggagaaaatt
    11461 gctaaagtag taaaaacagg atttggtgct gcggtaggtt ccggagatca atatatgtcg
    11521 tgggtacata tacatgattt atgcagaatt tatttatggg cgatagaaaa tgaaaaagca
    11581 gaaggcgttt acaatgcggt gtcaccaaac cccgttacaa ataaagagtt tacacgacag
    11641 gcagcacaag ccttaaagag accctttttc atgccaaatg ttcccggctt catactgaga
    11701 ttaatgtacg gtgagattgc atccgctcta ttaggcagtt ccaggatttc atcggagaag
    11761 attcagaatg aaggatttca atttgattac ccaacactga ccgaagcatt gcgggaaatc
    11821 tgatgagcaa aatttcaaat agtggacgaa cattaactgc aaattattgt aaattgattt
    11881 tataattcaa gggaattgca acctgggaac tgcggaaaga aagacttaaa tcgattaata
    11941 tgaaggaaga agcaaatgaa tccataaaaa ccaatggagc tgatgaagac ggtgaaagca
    12001 aaatcagaaa cgctttcaaa gacagggatt ggaatgaaat aaagagttca gattcgtggg
    12061 ccatctttaa agttatgtca gaatttgttc agggatttga gaaattgtcc caaataggac
    12121 cgtgtgtttc tatttttggt tccgcaagaa caaagcctgg tgcaccgtat tataaaatgg
    12181 ctgaagaaat cgctgccaaa cttgttcggc atggctatgg ggtaataacg ggtggaggtc
    12241 cgggtattat ggaagcgggt aatcgcggag cacgcagcga gtcgggaaaa tccgtgggct
    12301 taaatattga attgcctcat gaacaattct caaatcccta cattgattcg gataaattga
    12361 tctccttcga ctattttttt gttagaaaag taatgttcgt aaaatacgcc cagggatttg
    12421 tagtaatgcc gggaggcttt ggaactctgg atgaaatgtt tgaagctgta actttgatcc
    12481 agacaaagaa gattggaagg ttcccaattg ttcttgttgg caaggaatat tggcagggat
    12541 tgtttgactg gataaagaaa gtggccattg aggaaagtgc aaacgtaagt cccgaagatt
    12601 tggaactggt gaatattgta gacaatgcca cagatgctgt caaggtgatt gatgaattct
    12661 attccaaata cctgttgtct cccaacttct aattttcagt ttgaaataca taaatacata
    12721 cctttccctc gcgaccctga taattctgat tggatatatt ttctatgatc acgctgaggt
    12781 cgaaaaagaa actcccgcac gaattacagt ggcagaacct gattggaact ttcctggtgg
    12841 tgctaaatcc tttgaccttc cggatactat ttcattcgcc ggagaacggg ttcccctcga
    12901 aatacctgat gttcgtgaac ggctcgacag agaaatccat atcaatacat actggcacaa
    12961 caataccatt ttcatgatca aacgggcaaa caggtggctt cctcagattg aaaaaattct
    13021 tcgtgaagaa aacattccgg atgatttcaa atacatagca gttatcgaaa gtggattgat
    13081 caatgacatt tccgaaaaga atgcagtcgg tttttggcaa ttccttaaag gaacggcaaa
    13141 agattttgga ttaaccgtga atcgtgatgt tgatgaaaga tatgatccaa tcaaatcaac
    13201 tcgtgctgcc tgcaaatatt ttaaacaggc gtatgacaaa tttaacaatt ggacgctcgt
    13261 atcagccagc tacgacagag ggatgcgggg aatgcaaaac gccatggata accaaaaggt
    13321 agactcttac tatgacctgt tattgaatga agaaacctcc agatatattt tcagagtatt
    13381 ggccctgaaa gaaattttgt ctcatcccgc caaatatggt ttccaggtaa aagatattca
    13441 tttgtatgaa cctgaaccaa caaaaaccat tttggtttct gaagacatcc cggacttaat
    13501 agaattcgcg cgaactcagg gaatcaatta taaaattctg aaaaggttga atccatggtt
    13561 aatcagagat caattatccg tttctcgtgg tgagtcattt gaaatcgcga tacctgccac
    13621 aaattaattt tctaatacct gaacttgctc ttattatttg taattttgcg cacaatttca
    13681 gcaagtgggg gcgagaaaaa ctaaatccgg aacgaactca accaatggct ttgacgcgac
    13741 tggccatgaa tcgatagaga ttttcggtgc cagggagcat aatttaaaga atatcgatct
    13801 caagtttccg cggaacaaac ttgtagtaat caccggaatc agcggcagcg gaaaatcttc
    13861 cctggccttt gataccattt atgcggaagg acagagaaga tatatggaaa gtttttctgc
    13921 ctatgcacgc tccttcatcg gaaacctgga acggcctgat gttgataaaa taaatggatt
    13981 aagtccggtt atatccatcg agcaaaaaac tacatcccgg aatccaagat caacagtagg
    14041 cactgtaact gaggtatatg atttcatgcg gctccttttt gcaagagctt ccgaagcatt
    14101 ctctcatgtt acgggaaaca agatgattaa acaaacagaa gatcaaatcc ttgaaaagat
    14161 cctcgaacaa tttaaatcca aaaaaatcac aattctcgcg cctgttgtaa aaggaagaaa
    14221 aggtcactac cgtgaattgt ttcttcaaat ccgaaaattg ggatataaca aggtacgtat
    14281 tgatggggaa ctccatgatt tagtacctaa aatgcaggta gaccggtata aaattcatga
    14341 catcgaagtc gtaattgaca gggttgaggt aaaagacaaa gaaaaattca gaatcaatca
    14401 atccttaaat cttgccatga aggaaggaaa aggggtgatt atgttaatgg aggaaaatgg
    14461 ctctactcat tacttctcga aatttcttat ggatccggaa agcggagttt cttatgatga
    14521 gcctgctcct aatctttttt catttaactc accttatggg gcttgccagg tttgtaacgg
    14581 gctcggagca gtggaggaaa ttacccggga atcggtaatt cctgatagca atctaagcat
    14641 tagcagggga gctattttac ccctgggtgt ttacagagac atctggattt ttaagaaaat
    14701 agatgcaatt ctgaaaagat ataaattcaa cctgacaact cctgttaaaa atattcagga
    14761 tgaagtagtc gatattttac tatacggaga taaagtcccg gtggcagttc cttcagtaaa
    14821 atatccgggt acagaatgga atacaaaatt tgaaggtatc atcaatttcc tggtaaagca
    14881 aaaagatacg ggtaccgaag caattcaaaa atggattaat gaatttacaa tttccaaaac
    14941 ctgcccggaa tgtgagggag cccgacttaa gaaagaatcc ctttgcttta aaattgatgg
    15001 caaaaacatc gccgaacttg ctacttatga tctaaaacaa ctagcggatt ggttcaggaa
    15061 tcttgaggat agactttcag aaagtcaaaa aaggattgca tctgaagtgc ttaaggaaat
    15121 cagaaagcgg cttggttttt taaccggtgt aggtctggaa tatcttcaac tcaacagaac
    15181 tctcagaagt ttatccggag gtgaagccca gcgaatcaga ttagcgaccc aaatcggtac
    15241 ccagttagtt ggggtgttat acattcttga tgagcccagc attggattgc atcaaaggga
    15301 taatattcgg ctcattcatg cacttcaaag cttaagagac ctgggcaaca cggtaattgt
    15361 cgttgagcat gataaggaaa tgatggtttc ctcggactac ctcattgata ttggcccggg
    15421 agccggaagg catggtggag aaattgttgc tcaaggaaag cctttgcaat tccttaagaa
    15481 cggcagtata acagccgctt atttacgggg tgatttggaa atttcaaccc ccgaaaaaag
    15541 aagatcagga aatggagaga agataattct gataaaagct tcgggcaata atttgaatga
    15601 tctcacggtt gaatttccct tagggaaaat gatttgtgta accggagttt caggaagcgg
    15661 aaaatctacg ctcattcatg acactctgtt cccaattctg aatcagcatt tctactcatc
    15721 aagattagaa cctttgccct ttgccggtgt taaaggtctt gaaaagattg ataaagttat
    15781 tgaagtggat caatctccca ttggcagaac gccgcgatca aatccagcta cctatacagg
    15841 tgtattttcc gacattcgat ctttgttttc agaacttccg gaagctaaaa tccgcgctta
    15901 taaacccgga cggttttcgt tcaatgtgaa gggcggaaga tgtgaaacct gtgagggtgc
    15961 cggattaaaa ttgattgaaa tggacttcat gccgaatgta catgtccctt gcgagacttg
    16021 caaaagcaaa cgctataacc gtgaaaccct tgaagtaaga ttcaaaggga aatccattgc
    16081 agatgtcctg gacatgaccg tggatgatgc ggtaatcttc tttgaaaatc aaccaagaat
    16141 acttcgcaaa ataaagactc ttgaagaagt gggacttgga tacatctccc ttgggcaaca
    16201 tgcaaccacc ctttcggggg gagaagcaca gcgggttaaa ttagcaacag aattgtcaaa
    16261 gaaagatact ggtaaaactt tctatattct ggatgaacct actaccggtt tgcattttca
    16321 ggatatccgg catttgcttg acgttctcaa tcaattggta gataaaggga atacggtttt
    16381 gatagttgag cataaccttg atgttatcaa agtggctgat tacattatcg atcttggacc
    16441 cgaaggcgga gaagaaggcg gaaggatagt tgcactagga acaccagaat cactggttaa
    16501 atcaggcaaa ggctatacct caaaatatct taaatctgaa ttaaagaaca aaagcgctac
    16561 cgtgtagtag cgctttcact caaccaaaac aaatattaaa actatttgaa ctgtcaaata
    16621 tataaatgga ttttttacaa aaaggttaca tcaatttgaa tctttttgaa aaaaatagtt
    16681 aacactaaag atgaattctc aaaaactttc aataccggac acaacaaaaa aaagaattat
    16741 aattatcggc ggtggatttg caggaatcaa tctcctcaaa aatttgaaac atcagaattt
    16801 tcaactggtt ttaatagata aaaataattt ccacactttt cagcctttat tgtaccaggt
    16861 agcaacagca ggactcgagc ccgactcaat tgcagggcca ttaagaaaac aaatcagcgg
    16921 gaattatgag tactacttca gaatggcttc agtgttggac attgatccgg taacaaagaa
    16981 ggtgctgact gacctggggg agatcaattt tgatattcta atcattgcaa ctggaacagt
    17041 atcaaacttc tatggaaata aatcagtgga acaaaatgca tttccattaa aagaggtaaa
    17101 cgatgcatta aatctgagaa gtcacattct gcaaaacttt gaacatgccg tactgattga
    17161 cgaccaggaa gagctggaaa aatatatgaa tattgttgtg gtaggaggtg ggccaaccgg
    17221 agtggaggtg gcaggagcgt tgggagaatt gaaaagacat gttctgccaa aagattatcc
    17281 ggaattggat ttttcaggaa tgaatatcta cctggttgag ggtcttgaaa agctgctggc
    17341 aggaatgtca gaaaaagcag gaacaacagc cgtaaaatca cttagaaaat tctcggtaaa
    17401 tgtggtgctc aatacaatgg taaaagagtt tgatggcaat cgagtgctgc tgtccaatga
    17461 aaatgctatt gaaaccagaa ccgttatttg ggctgcagga gttaaaggaa caattttcaa
    17521 tggtatttcg aaagagtcac aactcaaagg aagaatattg gtagatagat ttaataaagt
    17581 acggggaacc gaaaatatct atgccgtggg tgacatctcc gtaatgcaga cgcaagcata
    17641 tcctgacggc catccaatgc ttgcccaggt ggcaattcaa caaggaagac tcctgggaag
    17701 gaatctgaaa agaattgaaa aaagcttaaa tccggtacct tttgaatata aggataaagg
    17761 tagcatggca acgattggaa ggaataaagc ggtggctgat ctgcccggca atatgcactt
    17821 tggcgggttg attgcctgga tgatttggat gttcattcac ctggtttcaa taattggttt
    17881 ccggaataaa attgtgattc taagtaactg ggtgtggaat tatttcacgt atgaccgggg
    17941 aacgcgactg attattcgaa aatttattcc taattgggaa aagaaaagtt cataaccatt
    18001 tcaacaaatt ttgcagccaa actttttcta aatgcgtatc ttagcttaaa tgatttcact
    18061 ctcaaagaac aaggtttatt ggattttcca gattgtgggc tggacagttt acacgattct
    18121 taatattctg ggtggtgccg caaattatgg cggaaatatt tatgcatcaa aacctgcctt
    18181 tctggttctt tttatttcag aaggaataat tttcttcctt attacacatt attatcgaaa
    18241 tctgattact aaacgcaaat ggctaaatga tggtaaaata agaattttct ataaggtgat
    18301 agtctccagt attattatgg gactcttaat atatctgttt agagttgttg tttcacaggt
    18361 gacttcaata gcagatccca aactgtataa attagcgacc atgcttggtc tcacttccat
    18421 taataccatt atcttatttg tttggtcact tttttatttc gtttaccact actttgaaag
    18481 gtttagcgca agcctgaaat acgaagccgc gttaaatgaa atcgccttga accaacttaa
    18541 atctcagctg aatccgcatt ttatatttaa tgccctaaat ggaattcggg cattggttga
    18601 agatgaaccc gccaaagcta aaaaagcaat tacgcaatta tcaaacatcc ttagaaattc
    18661 attggtcaca gataagaaac ggttaacgaa ttttgatgaa gaattgaata cagtgaaaga
    18721 ttatcttggg ttggaaagca tccggtatga agaacgactt caaacttttt atgacattga
    18781 tcctaaatca ggaagatttt cggttccacc tttaatgctg caaactttag ttgagaatgg
    18841 aattaagcat ggtatctcta cactcaaaaa aggtggtgtt attaaactga caactgtggt
    18901 taaagattca cgaatggtta tcagaataag aaactcagga caatatttaa atggcaaatc
    18961 aacaaaaaga aaagaaggat atggaataag caacaccttg caaaggctaa atcttattta
    19021 tgggaatgaa gcaacttttc gaataggaaa cgaggatcac aataccgtat taactgaaat
    19081 aaccctaccg ctcaatatat gaaagccctt ataatagatg atgaaagact ggcacgaaag
    19141 gaattactaa gcctccttga agaacataaa caaattgaag ttgtaggtga agcagctaat
    19201 gccgacgaag cttatgaaat ggtaaataga cttaaccccg acctcctttt cctcgatatc
    19261 caaatgccgg gaaaatccgg atttcaattc ctcgaatcat tggattcggt gcctatggta
    19321 atttttataa ctgcctacga tgaatatgca ctaaaggcat tcgaggtcag cgccattgat
    19381 tatttactaa aacctatcca gaatgaaaga ctggcggagg caatctccaa agtttcaaaa
    19441 ttcaatctgg ccaatgattc aaacgcaaaa agtgatcata aactgaaatt gacagacagg
    19501 gtatttgtta aagatggtga tcgctgttgg ttcgtaagtc tggcagatat ccggttattc
    19561 gaatccgacg ggaattatat caaagtttat tttgaaacat ttaaaccgat gattcataaa
    19621 tctctaaatg cccttgatga acggctggat caacgatctt ttttccgcgc aagtagaaag
    19681 cacatcataa acttgtcatg gttagattcc atcgaaccct ggtttaatgg aggacttatg
    19741 gcgaaactga aaggtggaga aaaaattgag gtaagccgaa ggcaagctgc gaagtttaaa
    19801 gacataatga gtctgtaagg cagatccatc aagttgtttt tcttgcatat tccagcaatt
    19861 ccttttcaat gccccaatcc ctggcaagtt tgaccatggc ccgtgcctcc atttctgtaa
    19921 tgaatccttt agaaaggttt gctctccagg tctcttccat atacttaatc tggttcgatt
    19981 tttttatctt cggaaattct attttaagat attcacgggc attctcaaag gcatgtatgt
    20041 agtctttaga aataaactca tcaacccatt tcaactcttc tcttccatgt atcaactcag
    20101 ctttttcgtt taataattcc tcctcctcct tatcaagacc atgataatgg aaaatcacag
    20161 atagcaaaag caagtatgtt tttttctcgg cagaatccat tggactggtt tttcaaaagt
    20221 agcaatgaaa atgagtaatc aaaattaaat ctttgacata ggaaaaagca agggctttga
    20281 agggcaggca tccgtttcaa gactgattat ttgcagatta acaagatata ggccatcggg
    20341 tactgaatcg ggcacaaaaa tcaattcggt aattgtcgca tttccgcggg ttgcggcagg
    20401 gaaattccaa aaattacggt gggccagaag tttcccgccg tcaacttccc tgtcaaccga
    20461 aggcaaatct accatcaaat gcaaaaagtt tctgtttgca agcatcttca acgccttggg
    20521 atcaaagtat ggcgggttgg ttccggaata ccttcgggct aattttccta ccgaatttgg
    20581 taatgacctc acgattaaac cttcaatatt cgtattggaa atttttgcct caaggctctc
    20641 ccatgttatt accgaatcat ctcccatcaa aatgggttca atggaaacca attcagaaac
    20701 gaagtgaaat tttttgagag taccattaat cgtcgcacca tcggcagaga tatgtccgta
    20761 acattctgta tgagttccat ttccatgcgg agttatggaa atcttttgat aattgaccgg
    20821 gccgccttca gagacactgc cgataaaatc acccgaaact attgtttcaa atacgggagg
    20881 ctctgcccaa taacaattcg gattactttc tccctccctc aaaggaatgg agatatctaa
    20941 agggttgctt aaatctgcag cgtatttttc tccattaaaa ttaaattcaa tcttcatgaa
    21001 aaagtttttc tgtaatatga tccgcgataa gtttgccctt atcggttagc accaacatgc
    21061 cttcactcac aataatataa tcttcagcta atatttgctc caaatatttt ttgtgaatgg
    21121 aatataagtc ataatcaaat ttagttttta atgaaaccaa atcacaaccc catttcgtcc
    21181 ggatggaagt catgattgtc tcattaattt tttgactccg cgaaagaacc tcacgttcca
    21241 ttggtattat tccctttctg atggaggcta tatattttct gttatttcga atattaaatt
    21301 gtctggactc accattataa ctgtgtgcgc caggcccaat cccaagataa gtggctccct
    21361 tccaataatt gctgttatgc ctcgattcat atcctggcaa tgcaaaattg gaaatctcat
    21421 aatgctcata cccatgatct ttcaaagttg ccatgatcac ctcgtattga atagcacttt
    21481 gatcttcacc ttcttcaacc agtttgcctt tcttaaccca attgccaaac acagtccttg
    21541 gttcaatggt cattccgtaa gtgctaatgt gttcaggcct gatttcaatt aacttgttca
    21601 gattatttct tatcatattt aatgaaccac ctggaattcc aaaaatcaaa tcaacactta
    21661 tattattaaa acctgcttcg cgggctaact gaagagaaat taatgcctga gatctgtcat
    21721 gagcacgatt cattaatctc aactccttgt catcaaaaga ctggatacca atgctgagtc
    21781 gattaatgcc cagtaatttc aattcattta agtatgaatc attcaagtca tccggattgc
    21841 actctattgt tatttcagcc tgctcgtcaa cattgtaatt actataaaca gcattcagaa
    21901 ttttttctat ttcttttgga gaacacaaag atggggttcc accaccgaag taaatagtct
    21961 taaggtcact tgctaaatat gaattttgta aagtgatttc agcgagcatg cattcaacca
    22021 tttctaccat gatactattg tcggtactaa aatggaaatc acagtagtaa caggcttgct
    22081 tacaaaaagg tatatgaaag tatattccca caactttagc taagaatttt gccaatttaa
    22141 tacacagtta ttaaagttac cggttaaccc gttatcttgc caaaagattt gagcataaca
    22201 aatttcatat tacccttttt gtggtctgca attatttccc tttacgctgt tccttccgtt
    22261 gttgaattgg cgtggaaaaa gggattaatg gattcatttg aaaagcggaa gattcatcac
    22321 ttacttactc ccaggcttgg tggagtagcc atttttgccg gtaccttttc tggaattttg
    22381 atatttaccc ggattgataa ttttcttcag tacttccttg tttcaattct gctgttattc
    22441 tttctcggag ttaaagatga tttaaatgaa atcaaaacct ggaaaaaatt gattgttcag
    22501 attttagcca cttctctcct gatttatgaa agtgaattta tgctgacttc cttcaaggga
    22561 ttacttggct ttgactactt ttacctcccg gttcaatatg ccctcacaat aatatatata
    22621 gtagcggtaa ccaatgcaat caattttata gatggattag atggactggc ggcgtcaatt
    22681 ggtgttatca tttctttaat ccttggtttt taccttttaa gatttaattc aatgttggca
    22741 atagcctgca tttgcctggg tggaagtctt ctgggattcc tgcggtataa tattaaagaa
    22801 tcagcagtct ttatgggcga ctccggttca ttgatcactg gatttatggt tgctgtatta
    22861 agtctaagtt tgatggagac taccaaagat gcctccggaa ttgtgattgc cttttcatta
    22921 ctgatgctgc ccgtaacaga tctggtcagg gttgtatttc tgcgtatatt tcaacggacg
    22981 tcaccattta cgccagggcg ggatcacatt catcacatat taattgattc tggttttacg
    23041 aaaaggaaaa ccctgctgat aattctttca ataacatcgt tctcgtcgct cacaggaatt
    23101 gccatggtta gcattttcca ttttgatcct aaccgtgcta tttttgtctt atttgtattt
    23161 tcaattttga ttacaatcta ttgtgaattt aaacgcagga gtttatttcg gtaaattttg
    23221 gatcttgatg ctgataacct tttccggtta tgctcagcaa tcagactcca cccggattca
    23281 aagcccaatc cttgatttaa gttatgggtg gatggagaat aaaaatggca aatttgaatt
    23341 aatccccttc aataaaaaaa gtaacgcgat ccacttttat atcagaagaa atattttagc
    23401 agaaggtgaa tttcttatgc aaattcctga gggaagttca ttgtggatcg acaacagatt
    23461 atttgaattt tttgaaaatg atacaacatt catcttttcc accaggcagt tccttgccgg
    23521 aaattccgaa cggctgattt ctatttatgg aaagaacctg aatcgtacca acctgcaaac
    23581 gaaattgatt aagactactt ttagacaaat gagagagatt tcacctcgac cccgaaagtc
    23641 agaccagaag tttcttctta tatcatttct ttcaggcctt gtcctaactg caatctttaa
    23701 tatgaatacc cacggaaccc tacaggggta tttttcaatt aaccgtactt tcaaccttac
    23761 aaatcgcgaa gagctaatat ttaagggcaa gctatttagc gccccaaacc cgattttcta
    23821 tttggtagtt agtttttatt cttccttcat ggttgttgca cttttgcttt taacaccaga
    23881 taccttttcg ataaatttta ggattaatac atttacttcc ggacaacttc tggtatttca
    23941 aagcatagta atgggaggat tttatatttg gatcgttgtt aaatggatgc tggttagata
    24001 tataggcggt ttatttcgat taactgaatt ctcaacaatc cattttttta attacgaaag
    24061 attctcattc cttttcctat tgtttatggc atcttgcctg gttgcttgtt tttttgcctt
    24121 tgggaatgaa accttcattt attcttttat ggtttcactt atagttttgg cagccacttt
    24181 ccggatactc cttatgctgc ttaaattatt gaagggctca ccctataaat ttctgcattt
    24241 attttcatac ctttgcgcca cagaaataat gccctatttg tttgctataa agttgatttt
    24301 aatctaacga ttaattggag taagaataga ccctatggat gttatggtaa aaagaagcga
    24361 ggggttgttt ccggtagaaa gactttttgc ggttaaaagt atactggtat ctcaaccgaa
    24421 accccaggat gttaattcac cttatttcgc ccttgcaaag aagtataccc tgaaagttga
    24481 tttcagacct tttattcagg ttgaacccgt caccataaat gactttcgta agcagaagat
    24541 tgacattctc gagcattcgg caataatctt tacaagccgg aatgcagtgg atcacttctt
    24601 taatatgtgt actcaaatga agattgatgt gccatcagac atgaagtatt tttgtatctc
    24661 ggaacaaaca tcgaattacc ttcagaagta tattgtaatt agaaaaagaa aaatttttac
    24721 aggcgataaa accgcgatgg acctgatcga aattctgaag aaacataaga acgagagata
    24781 cctgttccct tgctcgaata tcaggaaaga tgacatcccg ggttatatga aagaacaagg
    24841 gtataatttc cgtgaagcaa ttctttataa aactgttgca agtgatttat cagaccttgc
    24901 cgaaatcaag tatgatgtaa ttgccttctt cagcccgtca ggtataaata gtttgtttgt
    24961 caattttcca aaattcaaac aaaacaatac aagaattgca gcattcggtc cagcaaccgc
    25021 cgaagctgta aataaggctg gcttaattct tgatataaaa gctccgctac cagtggcacc
    25081 atccatgacc ggagccttgg agctttatat aaaaaaagca aataagttag gctgaaaatt
    25141 gcccgcaata agaaactaat tcaattagtt cctaatttta gaacttagaa atgagagcgc
    25201 actcacttgc tgtttttatc cttataagct ttggatttgg acttgaagtt ctgggccaac
    25261 aggatcccca attctcccaa tacatgttta actcgcttca ctataatccg gcttacgctg
    25321 gaattgaagg agtggcaaga attaccgcgg ttcacagaac tcaatggttg ggttttagta
    25381 catcctttga cggaaatgga ggaaacccga acaccgaaat attgaacttc agtttaccaa
    25441 tccggaaact tcggggcgga gccggcgtag aactgtcgaa tgataacctc ggcccacaga
    25501 acaattttaa agctttggca acctacgcat ttcattaccc aattaaagaa gctacgatta
    25561 gcatcggaat tcgggcaggt atttttgctc aaactataga ttacgatcaa ttccgacctg
    25621 ttcagcccga cgatccatta ttagtgaata aatccggcaa aacttcacaa gttaaacctg
    25681 atttggccgc tggcatttgg tatagtgcac caaaatacta ccttggagta gcattaaatc
    25741 acctgctcca atcccaattt gatttcggtg ttgccgggca aacaagtccg ttagaaccaa
    25801 atctcacaat aaccgccggt tatatttcca aagcgggcta caatttcacc ctcactccat
    25861 ccgtcctggt gaaaacagac cttaactcct attcattcga tttggggctg gtgggtacat
    25921 ttaaccagaa aatgtgggtt ggaggtgcct tcaggcaatc ggaagctgca atattattgc
    25981 tgggatatag ttttctgaag gatagggctc tgaaggtagg ctattccatc gacttaataa
    26041 tcaaagaccg cgaggccaaa cagcctactt cacatgagtt gatggtttca tatcttttac
    26101 ccttctcaaa tgagcccaga aaggccataa aaacccccag attcaattaa taagaataac
    26161 ttaatatttt gctttttaag tcaaatttgt acaatttgga gaagtaaaaa ttctttacta
    26221 gttaaaatta attataattg cttctaaaaa ttttcccatt aattcatcaa ggaattaata
    26281 ttttggtttt attttaggca ataaaataag ttaacttgga gttaattgtg taccaagttt
    26341 aattctgaaa gcgctatgga gaatcatttc agtataagaa aaaccttata ctttctggca
    26401 gtcattggtt gtctttcctg gataaccgga tgtggtcttc tgggaattgg aggtggagga
    26461 agaggtgacc gtggggagtt ggtaggtgtt ccggatcggg aaggttgggt aatgacaata
    26521 cctttcggta tggtaccaat tcccgcagga acttttcata tggggcaggc tgatgaagac
    26581 gttgctcatt cacagattaa cttcaacaag caaataacaa taggcagctt ctacatggac
    26641 gatactgaaa tcaccaataa tgagtatcgc caattcatga atgaaataat tgaagattca
    26701 ctaagtgtgc tcggcgaaga tttcgtcatg cgtgagttgt acccggacac cacagtttgg
    26761 gtaaaggatt tcacgcatca tatgggtgat cctatgatgg cttattacta ttggcatcct
    26821 gcatttgatg aatacccggt ttccggggta gactgggagg cagcaaaata cttctgcaac
    26881 tggagaaccc agtatttaaa tgactacaga caaagtgtcg gggatttccc gatgcctaat
    26941 tttcgtctcc cttcagaggc tgaatgggag tatgctgcaa ggggaggacg ggatatggca
    27001 aaataccctt ggggtaatcc ctacataagg aattccaaag gatgcatgct agccaatttc
    27061 aaacctggaa gaggaaatta ttatgatgat ggatttgctt atacctctcc tgttatgtct
    27121 tactttgcaa atgattatgg attatatgat atggcaggta atgtgtccga atgggctgaa
    27181 gatgccttca atccggcctc agttcccctg gtatgggacc tgaatccaac ctattttgat
    27241 gtaaatgaac ctaaaaaggt aattagaggc ggatcatgga aggatgttgc atttttcctt
    27301 gaaactggta ccagaacctt tgaatataaa gattcaacac gggcatatat aggatttaga
    27361 tgtgcaatga cccatttagg tcgttcttcg ggtagagaat ttcaataatt tttataactt
    27421 gtaaacacat aatactttta agtcttaatc aatctaaact ttaaacaaca tgagtagtaa
    27481 aaaaggcggt ttcgttgagc ttctttttgt aaccattatg cccaaggtct atggtatcgg
    27541 ggcggcggta gttattgttg gtgctatgtt taaaatattg cacttaccag gcgctgctgc
    27601 tatgttgggt attggcttaa ccacggaagc aattatcttc ttcttaagtg cttttgagcc
    27661 caagcactct gagcttgact ggacaaaagt atatcccgaa ttagcggagg attatcaggg
    27721 tggtgccggg gcccccagaa ttgccaacaa gaaagatagc ggagattccg taaccaagaa
    27781 acttgatcac atgctggaaa gtgccaagat agggccagag ttgatcgaca gtttgggaaa
    27841 gggaatgtcc aatctctcca aaaatgttgg tcaaatggct tcactttcta atgccgccgt
    27901 tgctactgaa gaatattcca agaatgtggg taatgcatcc aaagcattgg ttgatatgag
    27961 caagtcttat gcaacaaccg ccagcgcaat gtctcaaatg gcaaatgcct ctcaggatgc
    28021 gaaagagtat catagccagg ttcaaaatat tacgaaaaac ctgggagcac taaacgcagt
    28081 ctatgaaatg gaattgaatg atgcgaatag ccatattaag gctatgaata aattctattc
    28141 taacgtagct gcggttatgg aaagtatgga aaaagcaggt aaaggatcag agaggttttc
    28201 tgaagaaatg caaaaattga cctcaaacct gacttctctt aataacattt atggcaatat
    28261 gcttaccgcg atgcgaagtg gtggcgggca atccagcgcc gcacctaaag ctcagggata
    28321 agcggattat cgagaaatta tttttttatt tcaggcaaat taaaatgaat taattatggc
    28381 aggtggcaaa gaaactccta gacaaaagat gattgggatg atgtatctgg tgctaacggc
    28441 tttattggcg ctccagatta gtaactcggt gcttgagaaa tttatcttta tcgaccagac
    28501 gcttcaaaag tcagtagctg aaaatgttgg gaaaaacaac aagaccattg gaaggattga
    28561 agcagaggtc aagaagaagg gtgaccggga gggtgatgtc aaggctttgg aaaaagcgaa
    28621 acaggtgcgt caaatgacag cggcattgct cagtgatttg caggagtata gggatgaaat
    28681 ggcacgaatt accggaggtt acgatgataa tgggaacctt ataggttcca aggattatga
    28741 caaggtaggt acctggatga ttcacggggg gaagggtaaa gaattaaaag accggcttaa
    28801 taaatattct acagacttgg gatccgttac gggagaatca tttcttccat tagccaggga
    28861 tgcaaaggaa attgatttag ccaaaaatga tcccgaccaa aaggataaga atttctcaga
    28921 atactatttt gagaatactc caactgctgc cggaatggca accatcagtc agatggaaac
    28981 cgaggttctg agttatgagc agctggcatt agataaaatt gcagaagaag taggagccaa
    29041 ggatgtaagc tttgaccaaa tacgaccaat ggttcttcct gtttccaaca tagttgcagc
    29101 cggtgcaaag tttgatggtg accttttcat agcagcctcc tcatcagcag ttgatcctga
    29161 aatgaccttc gaaggaaagg aacttgaagt ggttgacggc cgtggaagaa tttcatttgt
    29221 tgcaacgccc ggagcttatg ataaagatgg atatgcaaag aagactttca aagcaactat
    29281 tacattaagc gactctacct acgatgttga acatgaatac atagtggcaa agcctgtaat
    29341 tcaaattcaa tcggcatcaa tttcttcgtt atacctgaat tgcggaaatg aactgaatgt
    29401 gcaagttccg gctttaggat cggcttacaa tccttcattc tctacaacag gagcggaaaa
    29461 tgtgaaaggc gcgcaaaagg gcattgtaac cgttattcca acggcaagaa aagtaaccct
    29521 gacagtaaga agcgatggca acttaattgg aagtcaggat tttggtgtca gaccaattcc
    29581 aaagccaatt ctcgagattt ctgaaagagg acgccctatc aatctcaaga ccggtgtttc
    29641 agccccaggt ccaagacaaa ttaatatcag agcggttcct gatgagactt ttctggctca
    29701 attgcctaaa gatgcgagat acagagtttc tgaatgggaa ataacccttg cagccggcgc
    29761 aagagcagtt catagtgaaa gagttcgcgc tgaacaattt gcaatgccac aaacaattct
    29821 gtcaagagtg caacccggaa tgagaattgt tattgaagta aagaggcttc aaaggcagaa
    29881 tttccaaaat aaagtagaag atgtatcact tgccggattg agtaatattt tcaccataaa
    29941 tattaactaa ttatgaagaa tgtgaagttg attttaggtt gggtactaat tatgatcctg
    30001 gttacgcctg cagcttattc ccaggaaact gtttccgaat acgatccaaa ttctgtaaaa
    30061 caaattccaa agtacgaaca gttttacaag aaaagagtgt ggcgtagaat ggatttgaaa
    30121 gaaaagcaaa atcaggcatt ttttgccttt aataatgaga taaccaaaat tattattgaa
    30181 gcagttaaag cgggcacatt gcagccatat aagaatgatt ctctgaaaac catgatgact
    30241 ttggaggaat ttaatgagaa tatgaaattg ccgggaattg acgatgaaga agatgatatt
    30301 tttggaggtg atactggcgg cggctggggt gatgatgccg gtggcggctg gggtgataca
    30361 ggcgaagggg aaggagcagt tgaagaggaa gtagttgcag tttctacgga attccttcct
    30421 aacgaggtaa ccttaatgac tctttgtgaa gaccagatat ttgataagag aagatctaca
    30481 ttgtactttg atattatggc tatcaccatt acgattcctg gtgacaggta tgaaacaggt
    30541 ataaacaagg atgtcggcac ctttaaatac tcagatttag taaggctttt taagagtatg
    30601 ccggaagaag ccatctggtt taatcgtcag aacagtgcgg aacatagaaa cctgtctgat
    30661 gctttcgaat tgcgattgtt cagcgccagg gttatcaaag tggacaaccc aagggatcaa
    30721 tatatctcag atgtttattc caggtaccca aaagaacata ttatggcttc ccaatggctg
    30781 gagctcgagt taatggagcg tgagcacaat ctttgggagt attaagaata tttagaaatt
    30841 cttgatttca gggaagattt actcttcctt tttttttgat aatactcaag aattctttga
    30901 gccctggctt tgccaaccgc tttctcgatt tctaatggag cagcctcatt tagttttttt
    30961 atagacttaa atctatgaag taagattgaa atggtttgtg gtccaattcc ttcgatattt
    31021 tctaactctg atcgagttaa ttgtttactc cgcttacttc tgtgaaaagt gatggcgaac
    31081 ctatgggttt catctcttat ttgctgaatc agcttcaggg aacccgagga cttacttaaa
    31141 tgtaaaggaa actgatcgcc tattgtatat atttcttcca gccttttggc tattccaatt
    31201 aaatggatat tactatcaag acctaaatct cgtaaggctt cgaaggctgc atttaattgg
    31261 cctttgccgc cgtcaattat tatcaggttt ggaaatgttt cacctgtatt cttaactttc
    31321 ctgtatctcc gtgtaactac ctccctcatg ctggcaaaat catcaggtcc ctggacagtt
    31381 ttgatgttga aatgtctgta ctcctttttt gatggtttac cttccctgaa acaaaccata
    31441 gctgcaacgg gagtagttcc ctggaaatta gaattgtcaa aacactcaat gtgttttggt
    31501 acctcactta gcctcaaatc attcatcagt ctaataaggg tttctggatc ttttgactct
    31561 ttctcatatt gcctttgtct ttcccgcttg tattgaaatg cattcttcaa agaaagttca
    31621 accaactttc ttttatcccc gcgcagcgga acgaagtttt tatatgcgtc tggcagaaca
    31681 gtcagtggtc tattggaaat aattgtggtt gaaggataac taaatgtctt ttgaatatca
    31741 aaaaccaaag aagttagaat gtccgattca ttttcatcca gtggaatttt aacttccctt
    31801 gtttgtgagg aaattatggc gccattctta atacgcaaat agttgaggta ggcataattc
    31861 tcatcagaaa ttatggtgaa gacatccgcg tctgaaattt tcctgttaac aaccaaagat
    31921 ttggactgga actttgtcaa gagtgataat ttctctttta acatctgggc tttttcaaat
    31981 tccagagaca ccgcataacg cttcatttct tccttaaaat gcttttctac taatccaatg
    32041 ttgcctttta atattgctgt tgcctgctcc atatcctttg cataattctc ttccatttgc
    32101 aaaccctcgc acggtccctg gcaatttcca atatgatatt ccagacaaac tttaaatttc
    32161 tttgccttaa tatttctctc acttaaatcc agtctgcagg ttctgattgt ataaaggctt
    32221 cgaatcaggt ccagtacatt gttcattgct acaacactgg aatagggacc gtagtaattt
    32281 ccgcttcctt taatgactct gcgtgtactg aatattctgg gaaatctttc gttggtgatg
    32341 aggatgtaag gaaaggattt gtcgtctctc agctgaatat tatactttgg ctgattttgc
    32401 tttattaaat tgttttcaag aagcagtgca tcaaattctg aatttacgat agtaaattca
    32461 atgaacctgg cttcagagac aagtttcaga gttttccgat tttcaaacag ggatttggtg
    32521 aaataactgc taaccctttt cttcagattc ttagctttcc ctacataaat aaccttgctt
    32581 gctttattca gaaattcata aaccccaggg tcgggaggaa gcagggcata ttccttggca
    32641 ttaagtaaat ttaccttcat gtggaatcca aaaaatctaa tcccgaatat cctctaacgg
    32701 tttttgaaaa tcaattgaat ctctttgacc aggggctcca tattttgagc aatccagttc
    32761 aacatttaag gaaaatgtgg gtcttgggaa aggacccttt gtgtatggta aatcctgatt
    32821 ggagtatact ttttgcataa acagggccca gattggcaag gccattacgg ctccctgtcc
    32881 atactctatg ttgcgaaaat ggatactgcg atcatcgcca cccacccatg caccggcggc
    32941 gagatccttt gttacaccca taaaccagcc atcagagtaa ttttgggtgg ttccggtttt
    33001 ggcaccaatt tcattgccat catcaagaag atgatattta taatcaagtc gtctcccggt
    33061 tcctccaggc tcttgaatag ttcctttcaa catgtgaagc atcaaatatg cagtttcttc
    33121 gctgattgcc tctcttcgcg tagggacaaa attttgcagt acattgccat atttatcttc
    33181 tattcttgaa atatagaatg gctttgtcca ggttcctctg ttcacaaagg tcgcgtaggc
    33241 tcccaccagc tcatacacag acacatcatg cgtaccaagg ccaatagaca gcacgggttc
    33301 catattgccg gttatgccaa gcctgtgagc cgcattcacc accgattcgg cgcctatttc
    33361 attagtaatg tggacggtaa tgctattctt ggattgagcc attccatttc ttaatgtaag
    33421 agtttgtcca gaccatttat tttcggcatt ctttggtctg tacggttctt tattgccagg
    33481 gatcacgtag gttttgggaa catcctcggc ttgataacat ggatagtaac cattctctat
    33541 cgcagatgta taaacaatgg gtttgaaagt agatccgggc tggttcttgc cttgtcgtac
    33601 gtgatcgtac tgaaaataat tataattgat tcctccaacc caggccttga tgtggccggt
    33661 aaatggatcc atggccatga accctgcatg caaaaatcgc ttgtaatatc taatggaatc
    33721 caggggactc aataaggtgt caatatctcc acggtaagaa aatacggtca tttctctggg
    33781 cgtattcatg ataattctga tggaatcatt tccttctccg tatttttcaa ccaaccgtct
    33841 atacctttcg gttctcttag ctacatcctg aataaatcct ttgatttccc ttccatctct
    33901 gtcaatccag ggatttttgc cttcccaatg ttcattaaaa attctttgca gagtatccat
    33961 atgctctgtc accgcttctt ccgcataaag ctgcaacctt gagtcaatgg tggtatagat
    34021 tttcaacccg ctttcataca aatcaattcc tctctcacgg cagaagtcga gcagatcaaa
    34081 taaaattaca ttgcgaaagt aaggagcaag accttcctta tggcttgcaa ctctatagtc
    34141 gagcacaatg tcctgaacgc ttaaggtatc aaattgctcc tggcttatgt atccatactt
    34201 taccatttgt accagaacca catttcttct tgctttggcc atctcggggt gatacctggg
    34261 attatttcgt gacggagatt caaggagacc aatcaacaag gctgcctcat ttatggacaa
    34321 actgtcgggt gtcttattga agaaagtttc agatgctgct ttaattccaa acgcaaggct
    34381 tccaaaagga acagtattca ggtaaagtgc aagaatttct tccttggtaa aagttttttc
    34441 aagttggatg gcaattaacc actctttgct tttggcaact attctccttg gataataccc
    34501 gaattttctc accgaaccct ccatttcttc tcccattgta ttataaattt tcttggcgag
    34561 ctgctgtgtt attgtactac ctccgcctct tgaacccaaa aaagcgactg cacgtagaaa
    34621 agcccataaa tccacgccgc tatgattctc aaaacgaatg tcttcttttg cgattaacgc
    34681 attaatcagg ttttgcgata gttgttcata agtcacctgg ctgcgattat acctgaaata
    34741 tttccccagt gacactccat ctgatgtaag cagttccgat gaaagatcgt tctccgggtt
    34801 ttctaaggcc tgcaatcccg gcattccgcc ataaaatccc cccaggtcta tcgcaactga
    34861 ataaaagtaa aagggaacaa ctacaagcag caagaaggca atggtccagg cggcaaaaat
    34921 gatgcgccgc caaatatttt tttctgaaac actgttttgg ttagtagctt tcattgaaaa
    34981 atttcaggta agcatcgaga tcctttgaat tataaagtat gtcgaagtta tctttagtga
    35041 tgataaagtt atgaaatttt actgtcctaa gctcggccgg cagattaatt tgcggaagaa
    35101 ttttgtcata aaagtccaca gctgttgact ttgaattcaa ttcacttact acgatcatcc
    35161 tccagccttc ttcaaaatcg agtatccctg tttttaagtt ttgatcctgg aatatttcac
    35221 gattaacaga gtcaactttt acggtcagct tagttaaaat gccttcgtca tttgtgttca
    35281 ccaggattac aaaataatgt tcatcctgag tgaacatgat gaatcttgcc tgcatggctc
    35341 tttcaatgcc ttgctcaaaa ttttctgctt tttcaagcaa tgttttggca aaagtggtta
    35401 catctgagtc aggataattt tcaatgaaag ccctgagcct gtttttataa agcgcaagat
    35461 cctctgattt cccggtaatt aaaatatcca ggagtcggaa acttgcatag aattcatttt
    35521 catcataaga agcaagggct tcacttatca agattcgggc cgagccaaaa ttcccattgt
    35581 cgaactgttc ataggccttt gcgtataatt gtctcaattc gccgagttca acagaagatt
    35641 ccaaggcata attaggattc acaattaacc ttgtgtaagt agaattagga tgctcttgct
    35701 ttagttgatt ttcgtaggat atatattgtg gatcttcttt ttctttcaag atcaagtata
    35761 aagtataaag aacctccggc tcaaactctg attcggggaa ccgggaaaga aatgttctat
    35821 agtttgtaat agcattatca gcttcgtgta gttcgaggtg ataaatttta cccaattgat
    35881 aatacgcttc ttcaatttta gccaatgctg attctttatc ttctttacta aagggaacat
    35941 ttttaatcat acttgccact tcagatgcta ttaattgatc ttcagaaaca acagttggct
    36001 tactttcttc ctcagctgag attgatgatc cggggttgga tctggaaaca atagcagcac
    36061 tctttgaact ccttcgccaa tcatcttcca atggccggcc tccccaggtt tttctgaatt
    36121 cattctgccc gagactaata gcggaaggat ttgtaaagta ccaggttaag gaggaagtat
    36181 tgatgccctg agtttggaaa gtgtttgtaa ctgtattgtc accggcatca gtttttttgg
    36241 aagcgatctc ctttttctcg ttttcaattc ggcttttaac ctgatttatg aaagttgcgg
    36301 acaattcagc tgtgtctaat cttgaaagcg caagcagact atcctgaagc tggatggtct
    36361 taagtttggt aacaaacctg tctaatacgt cttttctctt cactacttct tcataatact
    36421 cgaaggtatt cggcattacc tgaatagtac tgtcatagta ggactgagct ttttcaaagt
    36481 ttctgagaga atcatagtta acttgcccca ttctcagata tgataatcct ttttgtcttt
    36541 ggttagaaat actattttgc acagaaagag tgtagttttc aaatgccaga tctacattcc
    36601 cctgcttaag ttcaaactct cccatttcat aataaatctt atcctgaaac tcaatgtttt
    36661 ttgcatcggt cacaagcttt ttaaaatact tccgcaccct ctttacatct gatgtgttcg
    36721 ataactctgt aacctgggcc atattcaatt tcgcaaagaa agagagttcg taggtaggat
    36781 tattggccag gcatttccgg taattattaa aagcctcgcc ctggaatccc aggtgttggt
    36841 aaatctgacc aattatgaaa tagattttag ctgatccttc tttcttttta agcaggggaa
    36901 caaccaaaga taaattgtac accattttgt caaggtcttc gagctcctga tagagataag
    36961 cccgcgtcag atatagtttt ttcaaattcc ttttatttaa atcttccttt tcaagaaaat
    37021 ctgagacagc ttccgcgtta tttagctctc cgtattcaac aaaagttcta caaaggtgaa
    37081 tgagagacag gtgccgcatg ttgccatcct tgctattctt attcacccat ttaaaggtct
    37141 caattgcact ttcaaattca cccgtgtaca gccgggctaa tccgagaaga tcatagctgt
    37201 catccaccca tttactgttg ccatgattta cgatagccag cgaggtcttc ttaaccacat
    37261 cgttgagagt ttcttcaaat tgtttggtaa aggttgtatc aagcgggggg taaaccggga
    37321 ggaaatgatc ataatcgtct ttgtggtttt ttagtatttc actttcaacg cttctgattc
    37381 cattacgggc gtagtaatac gcattgtaat gtgctgttag attatgataa gagatgctaa
    37441 gcagtgtgtt tttctctgaa gaacaaccgg acgaatacat tgctgcaact attattaaag
    37501 ctgttattat ggtatttttc tttattcgca ccttaatttt tcacacaaag tataaacgca
    37561 aaaatttagt agatcaatac aatttgaata cggtaatccc tatttttgtt aaaattttaa
    37621 cgggttgaaa ccgaaaaaga cattttcaaa ttggctgttt aaccgctact ttctaataat
    37681 tagaaatgaa gaagactttg ctgaaaagag gtcagttagt ttcacctatg caaaggtaat
    37741 agttttgggg ttttccttct tagtagtggt aatgatattg agcttctttc tggtagaaac
    37801 tttgcttgca caatggttcg atccaaggca tgcccagatg gaggcaaaca ggcagctgat
    37861 ccggcttacc ctggaattgg acagccttga aagtgaagtt caaaaaaagg atttgttcat
    37921 tcataacttc caaaatattg tagccggaag aattaataat gaaggagcgg acggagacac
    37981 tgaaaataat aacattgatg aacttcaggc caagatagat gagatggatt atcaggccat
    38041 tgactcgcaa ttcaggaagc agtttgaaac caatgaggtt tcattattaa cttacaattc
    38101 cggcgagagg gctgaattac aggagttgtt tcttttttct cctatagatg gtattatttc
    38161 tcaaaaatat aacgttagaa atgaacacta tggtgtagac gttgttgcca agaagaatga
    38221 acctgtgaag tgcgttgctg atggaactgt cattttggca tcatggacac aggattcggg
    38281 atatattatt gcggtgcagc acagaggcaa tttaatatca gtttacaagc ataatgatgt
    38341 attattggaa aaagttggta attttgtcag cgccggagaa atcattagta tttccggtaa
    38401 cacaggcgag ttgaccgatg ggacccattt gcatttcgaa ttgtggtata atggtaatcc
    38461 tgtaaaccca gaagaatttg taacatttta aagactgtgg tatgtttaac aaagatgaaa
    38521 aaaaagtagc cgaagaaatc agcaactcca gcaacatcat tgggaaggga accattctgg
    38581 agggaaacat tgaaacctat gggaatatcc ggattgaagg aaaagtcata ggtaatgtca
    38641 aaaccaaatc aaagatagca cttggtcagt cttgcaaagt agagggcaat atcctggctc
    38701 aaaatgcgga agtagccggt gaagttgctg gccggattga agttggagag caattattgc
    38761 tgagaccttc agcagtaata aacggggaca ttattaccaa taaattaatc gtcgagtcag
    38821 gtgccacttt taatggagga tgcaagatgg gggttaagat caaggaaata cgcattggtg
    38881 aaaatggaca atccgcaaca atcccatcaa aagaagccta caaagcagta taacgactat
    38941 ttgaagtatt ccggtctggc ttttcaaatg atggccaccg ccggaattgc cacatggatt
    39001 ggctacaaaa ttgatcaata tctggaatta agattccccg cgtttaccat ttctttactc
    39061 ctggtttcac tcatcggtgt gatttattgg ctgataaaat ctgtaactaa taattccgaa
    39121 aaatgaggaa gagtttcctt tcattcctga ttaatcatac tattcttact cttcttctcg
    39181 cagttttgtt catcgctata aaaattatgt ttccaggctt tccaattccc gattcaatat
    39241 tcatcatatt cgtgtttttc tttgttttcg ttttaatggt tcatgggtta ttactaagag
    39301 ttgcagagaa gaaggcgcag gtatttatta gagtgtttat ggtaatgacc ttcgtaaagt
    39361 tatttgtcta cctgggtttc atatccatcc tcattctgct ttacaaaagt gatgcgaaag
    39421 gaattgtcat tgtttttggt gccttgtatt ttgtcaatct ggctcatgaa attaccgcga
    39481 tattgaggca cctggatcaa aagaaaagtg agcagagaga agtataaaat cccttccaaa
    39541 aattaaatca ctataacttt tgccttattt attattgctc taaatttgca ctttgatttt
    39601 cagaccccta aagcctgcga atgacagaga gaattttgac ccttaaaaaa gcccgaaaat
    39661 acctcctctt ttctctgtta atttgctctt ttttattggc aaactctacc gcttttgcac
    39721 aggaagaaca tgcagaatca gagtccttcg atgcgggcaa gatgatcatg gaacacattc
    39781 tggactcgca tgaatggcat gtttttacct ttgggcattt tcatggaact gtacccttgc
    39841 cggttatctt atactcccct actaatggat tggctatttt catgtcaagc aagtttcatc
    39901 acggagagga aacttacgct gggtataaat acgaagaagg aaaaattgaa tcattggatg
    39961 gaaatgtttt ttatgatatc tcaattacca aaaatgtagt ggcaatgctt ttcagcatgg
    40021 cattgatgtg ctgggtactc atctctgtcg cgaatactta caaacgaagc cccaacaccg
    40081 cgcctaaagg cgtccagtca ttgcttgagc caataattat ttttgttaaa gatgaaatag
    40141 caaagccatc cattggcgag gaaagatatc aacggttttt acccttcttg cttactgtgt
    40201 tcttttttat ttggttgaat aatatgcttg gcttagtgcc catcattccc ggcggagcta
    40261 accttaccgg gaacatcgcg gttaccatgg tcctggctat gttcaccttc gtgatcacta
    40321 ctttcagcac caacaaagga tactgggttc acatttttaa tacacctggc gttccctggt
    40381 ggttaaagtt tcccatacct attatgcctc ttgttgaatt tatgactgtt ttcaccaggc
    40441 cctcggtatt gctcatccgt ttgtttgcca atattaccgc aggtcatatt atcgcgttgg
    40501 tttttgtttc catgattttc atttttggag aaataaatat tttcctgggc tatggagtag
    40561 gagtattttc cgtattattt aacgtggcta tgactttcct ggagttactt gtggggttta
    40621 ttcaggctta cgtatttaca ctgctatccg cattatattt cggaatggcc aaagttgagg
    40681 aacatcatta aatgtgtaat ttctaaaatt tatatatatg tctttattta ttttattaga
    40741 tgctgcaatc ggttacatgg gagctggcat cggtgccggt ctggctgcaa taggcgctgg
    40801 aattggtatc ggtcgaattg gcggtagtgc gctggagtca atagcaaggc aacccgaagc
    40861 gattggtgat attcgtgcca acatgattgt aactgccggt cttattgaag gtgttgcaat
    40921 tttcgcggtt atcgtatcgg tattaattat tttccttact tagaaaaggg aatgcagcat
    40981 gcattattta caataggtat taatgcatgc tttttaagaa cttaagaaaa aagctatatg
    41041 gaactgatta cccctgattt aggattagtt gtctggatga caatttcatt cagtcttgtc
    41101 ctttttattc ttacaaaatt tgcctggaag ccaattgttc aaaccttgaa aatcagggaa
    41161 gaagccatat cggaagcttt gaaatcagcg gatcttgcca aagaggaaat gtctaaactt
    41221 aaagctgata atgaaaagct tttagaagca gccaggctgg agagggataa aattctcaaa
    41281 gaagccattg ccgcaggcaa caaactgaag gatgaagcca aggaagattc tcagaggatc
    41341 ggcaaaaaaa tgatagagga tgcccgggtt actattcaaa atgaaaaaaa gactgcaatg
    41401 gaagatgtga agaagcaact ctcgcaattt tcaatggaaa tagctgaaaa gatcttacga
    41461 agggaattgg aaaataagaa agcccagcaa aaactggtga atgaatatat taaagatatg
    41521 aaggtaaatt agaatgtcag cccgagcagc atcacgatac gcaaagtcat tgctggaact
    41581 tgccagggac aagggcaaac tggatgaggt tcatcaagac atgttgcttt tcaataaagt
    41641 atgtgatgaa aatttcgcct ttgtcctgat gctccggaat ccaattataa accatcctaa
    41701 aaagcgtgca gtattacatg ggctttttaa atctaaaacc gacccattaa caatggcaat
    41761 tctggacatt actaccagga aaaaccggga aaaattgttg cctatgatcg cgaaagagtt
    41821 ccattaccaa tacaacattg aaagaggaat tcaggaggct agtattacta caacttttga
    41881 gcttgatgat tctctccgga agaagttcaa cgaaattata atagagataa gtaaagccaa
    41941 aacattatca cttgaggaaa aaatagatga aaatattatt ggtggatttt tacttaaggt
    42001 tggcgataag caagttgatg aatccatatc caataaactt aaacaattac gattgaagct
    42061 cagttaaaag cgagagagaa aaattcaaac aataaaaaaa tggcagacat aagaccagat
    42121 gaagtatcgg caatattaag agaacaatta tcgaatttta gaagtgtagc tgagctggag
    42181 gaagtaggta ccgttctaac tgttggtgat ggtgtggcaa ggatttatgg gttgtcaaag
    42241 gttcagtcag gtgaattgct tgagtttgaa aacggactcc aggcattggc ccttaacctg
    42301 gaagaagata atgttggagc tgtaatcctt ggtgattcaa gatccattaa ggaaggagac
    42361 accgtaaagc gaaccaaaaa gattgcttct atcagggttg gtgataaaat gtgcggccgg
    42421 gtagttgata ctcttggaaa acccattgat ggtaagggcg caattgatgg ggaacttttt
    42481 gagatgactc tggagagaaa agctcccgga gttatttaca gagagccggt gagtgagccg
    42541 cttcaaaccg gaattaaagc gattgattcc atgattccga ttggccgggg tcagcgcgaa
    42601 ttgataatcg gagataggca aaccggcaag accgctgttg ccattgatac cataattaat
    42661 cagaaagagt tttacgaaaa aggtgagact gtatattgca tatatgttgc cattggccaa
    42721 aaagcgtcca cagttgcttc tgttgtttct tctcttcagc gccacggcgc aatggattat
    42781 acaatcatag tttccgctac cgcttctgat cctgcaccaa tgcagttttt tgcaccattt
    42841 gccggggccg caattggtga atatttcaga gatattggca aacctgctct ggtaatttat
    42901 gatgatttat ctaagcaagc tgtcgcctac cgtgaagttt ctctgttgct cagaagaccc
    42961 ccgggacgag aagcatatcc tggagatgta ttttacctcc acagcagact tcttgaaaga
    43021 gcggcaaaaa tcaaccgaag cgatgccatc gctaaacaga tgaatgatct gcctgcttca
    43081 attctggata aggttaaagg cggtggatcc ttgacagcgc ttcccatcat tgaaacacaa
    43141 gccggtgatg tatcagccta cattccaact aatgttattt caataaccga tggtcagata
    43201 tttcttgaaa cggacctgtt taattcaggt atcagacccg cgattaatgt gggtatttct
    43261 gtatcgcgag ttggaggtaa tgcgcagata aaatcaatga agaaagttgc cggcactttg
    43321 aaacttgatc aggcgcagtt cagggaactt gaggcattcg ccaaatttgg atctgacctt
    43381 gataaagcta cgcaactcac cattgacaga ggacgtagaa acctcgaagt tttgaaacaa
    43441 ggccagtatg cgcccgttcc ggttgaaaga caagttgcta ttatttatgc ctccactaaa
    43501 ggactgatag ataaagtgcc tattaataga gtgagagaat ttgaggctga atttttgacc
    43561 attcttgagg cacagcaccg acaggcactt gatacgataa aggcaggaaa acttgatgat
    43621 gatgtattag aaactttggg aaaagtggca aaggagttag ttccaaaatt cgcaaagtaa
    43681 agttaaaaaa aagggcgtgg caaacttaaa agaagtaaaa aaccggatta gttccgttat
    43741 ctcaactcag cagataacca aggccatgaa aatggtagcc gcggccaagt tgagaaaagc
    43801 tcaggataag attattcaaa tgcggcccta tgcccaaaag cttaactcca ttctgcaaaa
    43861 tgtagcttca ggaaacctaa acgtagatga caacgatttc gtccaggtaa gagaagagcg
    43921 cagcgtcctg cttattgtaa tcacttcaga cagaggatta tgcggtgcat ttaattcgaa
    43981 tgtaagcaaa gctgctctta ggcggatgaa ttatcttgaa gaacaaaatg gctcgacagt
    44041 aacaattctt cctctgggaa aaaaagcaaa tgatttcttt acaaagagaa aggcaaaaaa
    44101 agtggtggac ttcttatcta ttttctccga gttaaatttt gaaagcgccc gaaaagttgc
    44161 tgatttcgtt atgaatgact tccgcaaaaa agagtttgat agggttgaga tcatttacaa
    44221 tcaatttaaa aacgttgcca cccaaatttt gaccacagaa caaatgcttc ctattcaaca
    44281 agtgggagga gaaggcggaa gaactcttga ttatatcttc gaaccctcca aagaatacat
    44341 tgttcgtgaa ttaattccaa cttctttaaa aattcaactt tacagggcat tgctggaatc
    44401 aaatgcagct gaacatggtg cgcgaatgac cgccatggat aaagccactg aaaatgcagg
    44461 tgaccttctg gataacctgc gattggatta caatagaaca agacaagctg ctattaccaa
    44521 agagctaagc gaaattgtag caggagcaga cgcactctga taattaatta tggtttaatt
    44581 attaccttcc tataaggctt aataacttag gcttatgaaa gctattgcca gaactttcaa
    44641 ttccctcagc ctaattatag ttgctggtgc atgcatctcc agcatattca tctccagagt
    44701 tcttgaaagc gaactttccc tcctgtcaat aatttccctt ggtatttcag tttggattat
    44761 ctacaccttt gatcattaca ttgatgcgaa acaacttaat aatattaaag ggaagccccg
    44821 tcatcaattt catctggaaa acctggcctt aatcaagttc ttactgatag ccgcagtatg
    44881 tgtgaatact gtcatcttaa tttttctacc aaatgaggtt ttaataatgg gaataatatt
    44941 gctacttttc ataacaggat acttcatgat tattaagtta ccaattcaat acattggtgt
    45001 ttttaaggag ctaattattg cggctattta ttctgcggga atttttattc cggcaatagc
    45061 aacccacagt aaaactgttt caatttcaat aatcccttta tacctggtat tcttccttct
    45121 ggcctggatt aatttattgc tattctcatg gtttgatttt gaatatgatc gacgggcggg
    45181 ctttgcgtcc ctggttattg tattaggaag agatttttca aataagataa tctggattct
    45241 cttgataatc ttttatatac tttggatttg gactttttat ttcttcacaa gtggatcatt
    45301 tccaggcgaa cttaagtgga taacaatagc aatggcaatt atcctgtcag caatgcaaaa
    45361 taacaagcgg ctattttaca agggagagaa ttatcgatta gttggtgatg caatttttct
    45421 tctgccactt atggctctcc tgaaatgaat tcttacgcgg atttttctgg aattgccttt
    45481 gcttatgatt acctggccag gatatttttt ggtgatacaa ttaagagatg tcaaatttgg
    45541 actctgaaaa gtcttcaaga aaatgccact gttttgattc ccggaggtgg cacaggctgg
    45601 attcttgaag aaattgacaa actgaatatt ccattggaaa ttgtgtatgt tgaaccttca
    45661 aaaggaatga tgagaaggtc aaaagaaaga aaaaccaata atctgaaaat tcactacata
    45721 aatgaaatga atggaagcag ctccacagaa tttgataaaa ttcttcactt cttctatctg
    45781 gatctattta atgaagtgaa cctggaagca gaaattatca gcgcacgaaa tttattgaaa
    45841 cctaaaggct tcctcctttt cgcagatttc caattaaatc gtaaaactct tccggtatgg
    45901 caaaagagtt tgtcttttat tatgcatcag tttttcagaa gggttaccgg tatggagtcc
    45961 aacaagctaa aggatttaca ttccgcgata cttaagagcg gatttaatct tgttgaagag
    46021 aagttttttt atggaaactt tattagcgga aagatttatc aaaaaattta atcctttgat
    46081 attattttga attcgaccct tcgattctga gccctgcctt cctctgtatc attggtgtct
    46141 atgggttcgg attcgccata tccgacggcg gttactttat cctctccaat gccatgttca
    46201 agcaggtaag ttctgactgc atttgccctg gcatcggata aaactctgtt atattcatcc
    46261 gatccctggg aatctgtatg tccgccgatt tcaatcacca ggcctggaac ttgttcaaaa
    46321 attttcagta gtctatctaa ttcagggaat gactcatctt ttaagatcgc tttatccaaa
    46381 tcaaagaaaa tattattcaa tcgtacggtt ccccctattt caatgggcac caggtacaga
    46441 tctttttcaa ttactgaatt ttttctaacg cgggctgtac ttatactatc atttatcgaa
    46501 atataatctt tagcttccgc gtagaaacca aattgatagc cgattggcaa aatagtttcg
    46561 taatacccgg attggtcttg tgaactgata tttacatatt cacttccagt gtctaaatct
    46621 tcaaaaacaa tatttgctcc accgatcgga ttacggcttt tctgattata aacggttcct
    46681 ttcaaagtaa attgattgca aggtaagaca tccctgagat acagcgcttt catttcttcc
    46741 aggctgagag atctgttata tatgcgtatc tcgtccaaat aaccttcaaa tgggcgattt
    46801 tgccttacga cctgccgccc ggctttaagc gtaatttccg aattggaatt ataaccgctc
    46861 cctgtctgct cccctgtttt gtccatctca ccattcacta agatcccaac ttttctggag
    46921 cgattccaaa ccagtataat atgataccat tgaaatggtt caaaaatggt tttgctataa
    46981 acaccatatc cgaactcctt tccatcagct acggcaaaag taagcaggtt ttgaacatta
    47041 ataaagactg caaactggtc ctttttggca tcaaatcccc acttaccaac tatcagctgg
    47101 aaatcttcag gatttaattt tgtagggtaa aataagaatg aaaccgtcaa acccgaataa
    47161 ggcatattca attgatcagg attaccaaag tcaatataat catctaatcc gttgaatttg
    47221 gctacaccat tgccacacct aaattccgga tcaagggttg ctccataaga ggttccgtga
    47281 ctgccaaatt cgctctggtc agaaaaatca ttgtcaaaac tgtaatgtgc catcaatccg
    47341 cgccttaaat caatactttg tgcggatgcg gcagaatagg aaattatcag aatgaaaaaa
    47401 attaaagcag cacttctgga catagattcg tatttaaatc taaaatacga aaactgagct
    47461 ttttaatcca aataaaaatc tctcagttat tcaccaagtg agcttcttac tttaatttca
    47521 gagtgcagtg ttttgtgaac cggacattta ttggctattt caagcagcct ttttctttgt
    47581 tcatcggtta attctccttc aaggtagatt actcgcgaga tagtgtctat tttaccctcc
    47641 gtagactcac aatctttaca atcttcagca tgtatttttt catgtcccag tttaacattt
    47701 acgccagtta aattccattc ttttctatct gcatacattc ttaaagtcat tgcggtgcaa
    47761 gaaccaaggg cagcaagcag aaaatcatag ggatttggac cgagattttg acctccggat
    47821 tcaaagggtt catcagcaat tagttcatga tttcgaatga taattttact cctatatttt
    47881 tccatgccga ttgaaacttc ggcgttttct ttaaaattgt gcattgttta atgtttaggg
    47941 caataataat aatgcagccg gaatccacaa atgattttac cccattagtg gggaatttca
    48001 aaattaaatt ttgtgccttt tcttatttat attgcagact caaaacctaa aggattttca
    48061 tcccaaggta attatcccca gcaatgaacc tagttgaggt cttaaatcca aaacaagaga
    48121 aattgtttat acagtttgcg gttcagcatt accggaatga acctaaatgg attaggcctc
    48181 ttgacaaaga cattcgtttt attttcaata aaaaaaagaa taaatcttac cggaatggag
    48241 gtgaaagtat ccggtggcta ttatataata acgatgaaat aattgggaga attgccgcat
    48301 tttacaatcc aaagctggct ggaagagata acgaacaacc cacaggagga gttggttttt
    48361 ttgaatgcat tgatgatcaa aatgccgctt ccatgctgtt cgaagtttca aaagactggt
    48421 tgaaagacaa aggaatggaa gctatggacg ggccggttaa ttttggtgac agagatgcct
    48481 ggtggggtct tcttgtggag ggatttaatg aagagcctaa ttaccggtgc aattataatt
    48541 acccgtatta ccagccgcta tttgaaaatt atgggtttca actttatttc aggcaattaa
    48601 cctatgcaag aaaaacagcg gatccattat ctccaaagct gcacatcaaa gcggagatga
    48661 tatcctctga tccaggatat agttttgcac atttcagaaa aaaacaatat aaaaggtttg
    48721 ctcaggattt tatgaatatc tataatcagg cctgggccgg tcatgtagga gttccgaaat
    48781 taactccggc tcaagcggaa ctttcaatga aactgttatt acccatagtt gatgagaaaa
    48841 ttatttggtt tggatattat aagaatgaac cggtggcatt tttcattatg ctgcccgaag
    48901 tcaatcaaat attcaaatat gtcagaggga aattggatat aatggggaag ttgatttttc
    48961 tatggcataa gtggaataag acatgccgca aaatgatcgg agttgttttt ggagtggtgc
    49021 cggagcatca aggcaaaggc gtagaaggtg ccataataat ggcagccagg aaaatggtac
    49081 aggatgatta cgcccggtat gaagacttcg aaatgaattg gattggagat tttaatccga
    49141 aaatgattcg tgtggttgag caggtgggag ctcatgtttc aaaagtgcac aacacctata
    49201 gatatttatt tgatcgaaac gtgcctttta aaagaatgcc aataaaaaac taaatgggga
    49261 agtggtataa agtatcaaga aagaaacgaa gatttcttct aaagaatctg ggcaaaggtt
    49321 tgctgtggct ggccgctatt gtccttctct ttgtcataat caaaagatat gttccaataa
    49381 atgatacatc ctggatatac aaattgtatg ataagccgct attggtttat ttgattttcc
    49441 tggcttcaga aattattgtt ggcattatac ctcccgaaat atttatgatt tgggcattga
    49501 agtccacaac agctaaattc tatgttgttg atataatttt actaagcaca atttcctatt
    49561 tcgccggcat actgggatac tttataggta aatggtttca cgattcaaga ttttacacat
    49621 ttctgcatgg aaagtatctt tatcagtatg aaaaatttct aaaacgattt ggcggattcc
    49681 ttgtaatagt tgccgcagcc acacctttgc ctttttcggg gatttgcgtc cttgtaggaa
    49741 gcgttaagtt taacttcaag aagtttcttc tttttgcgtc ggcgcgattt attcggtttg
    49801 ccctttatgg ttatataatc tggcaggcaa acacaatctg aggttattcc tgggaagtaa
    49861 cgtaaagttc agacaatcgt tcggctgcaa aaaggatttc atcttcggta tttaacttgc
    49921 taaaagaaaa ccgcactgcg ccccgttccg gatccgcgcc aatcgcttta agaacatggg
    49981 acccaaggtt tgacccgctt gtacatgcgc tgcctccgct tgcactaact ccaccaatat
    50041 cgagattgaa aagcagcatt tcattttccg gagactctgg gaggcaaaca ttcagaactt
    50101 tataatttga cttttcaaga ttagccgagt caccattgta gcttacacca tcaagctggc
    50161 catcgagttt actgatcaat agagttttca aattttcaat atgagaccgg tgtttattca
    50221 tatccctgta gcaaatttca agtgctttgg caagacctac aattccatac acattttctg
    50281 tcccacccct catatttctt tcctgggaac ctccatgtat gtagggatca atttttaacc
    50341 cgccccgaat atacataaat ccaatccctt tgggtccatg gaacttatgt gccgagccaa
    50401 ccattgaatg aagattaagt aactgtaaat tatgaacata gtgaccaaca gtttgaactg
    50461 tatcggagtg aaaatacgaa tcagattcct tgcaaagatt tccaatatat tccaaatcac
    50521 tgatattgcc tatttcatta ttggcatgca taagagtcac caatgaattg ggattttgat
    50581 ttaatagctc agctaaatgg ccataatcaa tatgtccttt ttcatccagg ttcacgtagt
    50641 ggatattggc aagcccctgg gatgaaatat gatccaaggt atggagtacg gcgtggtgct
    50701 caattcttga acttataaca ttcttaacac cgagagaatg aacaattcca cagataacag
    50761 aattgtcggc ctcggtgcct ccagaggtga agaagatttc agatggtgaa acatttagaa
    50821 gctcagcaat tactttcctg gccttctcaa tggctgacct tgattccctc ccatgagaat
    50881 gtatggaaga agggtttccg aaatgtgttt ttaaaaatgg ctccatagca gcgatcactt
    50941 cagtgtccag cggagtagta gaggcattat ctaaataaac tcgcataatt tatacttttg
    51001 cttttataat attttcaatt tgattgatgg tagtagcagc caatttcctg gctgtttcag
    51061 cgcttttggc ctcggaataa atacgaatga tcggttcagt attcgatctt cttaaatgaa
    51121 tccaatagtc ggcaaagtcc acttttattc catccaatgt atttatgtct tcgttcttgt
    51181 gaaggatgtg aaattggctt aatacttcgt cgacatcaat ggaaggatct aacagaattt
    51241 tgtttttgct aatgatatag tcaggatact gggatcggat ttcgctgact ttcttcccgg
    51301 atgctgccat ctgacttaga aaaagagcaa ttccagccaa tgcatccctg ccataatgca
    51361 agtcaggtat tattactccg ccattgcctt cgccgccaac caccgcatta gttttcagca
    51421 tttcttccac cacgttaatt tctccaactg cagctgctga atacttaccg ccatgcttta
    51481 gagtaatgtc tctaagcgct cttgaggaag aaagattgct taccgtattt ccaggataat
    51541 gggacagcca atagtccgcg atggagacaa gggtatattc ctcgccaaag aatgttccgt
    51601 cttcacaaac aaaagaaagc cggtcaacat ccggatccac agctattccc aaatcaaaat
    51661 ttccttttac cacttcggaa gataactcac caagattttc aggcaaaggc tccgggttat
    51721 gggtaaagtg tccgttcatt tcaccattta aaattttaaa ttcagtaatt cccaatcgtt
    51781 gcaaaagagc cggaactgcg atggaaccca ccgaatttac cacgtcaaca ataatgcggg
    51841 gtgccaatgc cttaattttt tctggagaaa ccaatttcaa ttcaagaatt ttattgatat
    51901 gataattaag ccactcttct gacctggaaa tacttcctaa ttgatttacc tgactgtatt
    51961 cgaagttgcc cgattctgca atgctgaaaa tttctgcggc ttcagcaggt gataaaaact
    52021 gacctttcga attcagcagt ttcagtgcgt tccaattctc cggattatga ctcgccgtta
    52081 taatgatacc tccgcccgcg ttacttccgg tcaccgccat ttcaaccgtc ggtgttgtag
    52141 cgaaatctaa atcaataaca gaaataccca tcgaagttag ggtggaggaa actaaatttt
    52201 tcaccatgag ccccgagggt ctggcatctc ttcccagtgt cacaatctta ttttgctccg
    52261 ttttgtttat ccatgtaccg tatgcgctgg taaaatttac aacgtccacg ggagaaaggc
    52321 cttcgccggg cttcccaccg atggttccgc gaattcctga tatggaacta attaaagcca
    52381 cgattacatt ttcgggcaaa gttagctatt ttttcggggg aaggaaaccc gaaattagtg
    52441 tcagaggact gaaagccatg gaatttctga tgctgacagg cggaacccca aaagcagtca
    52501 aaaccctaac gtttttccag tatgcttgtt taagccagtt accggatctg aaacccgtat
    52561 gcaggttttt cattattagt attttaattt tcagtttaac tatatatttg tcaataatct
    52621 attaaaatat aatgagtgtt ctatccgttc ctcttacacc tgatctcgaa caggcaatta
    52681 ataacctgct aaagtggggt gtagcaagta atcgcgctga tgctgcccgg aaagctatcc
    52741 taaaacttga ggaagatacc gccattcaag ttgttcttga aagtgagcaa gccgcactcg
    52801 agggaaaatt gatttcagga aacctgagag gttttatcaa aaaatcttcc actaaaaagt
    52861 gaagatttat ttcaccccgc ctttcttaaa gcaatataaa ttcacttgac cccccacaga
    52921 aactaatctt tgaaaggtta gtttctcttc aaaaaacaca aataatatcc taaaaatcta
    52981 atgattgtaa aggtctggaa attcagcaaa cttagcttaa gaagcctctt tggcgcacgc
    53041 ttcgccttca tttacttttt taccgcaata gctgcaggta acaccttctt tatttagaaa
    53101 tggactctga gaagcgcatg tgcccttaaa ctcaccgttc ttcagaaaga tcaatcgcac
    53161 cgacatcaaa atgaagaaca gcgcaacaat tccgacagct aaaagaaggg tggtcataaa
    53221 tcaatatatt tgactttcaa aattacggtt attcacaata aataacattc aggataactt
    53281 tatagtttca cattttcaaa aaagatgggt aaaatcaata aaacaacaga caacagaaga
    53341 caggagaaac tcaaggcttt tgacagactt ttgacaatca tggacgagtt gagagaaaat
    53401 tgtccctggg ataggaagca aacgaatgaa agcttgcggc atctcaccat tgaggaaact
    53461 tatgaattat cgggttcaat tctggagaac gattacaatg aaattaagaa tgagttgggt
    53521 gatttaatgc ttcatcttgt cttctatgca cgaatagctt cagaaaaaaa tgaatttgat
    53581 attggcgatg ttcttaattc agtgtgtgat aaattaataa agcgtcatcc tcatatttat
    53641 ggtgatgttg aggcgaaaga tgagaaaaca gtaaaggaaa attgggagaa gattaagctg
    53701 aaagaaggta attcagttct tggtggagtt cccaaaagtt tgccggccat gataaaagca
    53761 atgagaatac aagaaaaagc ccgcggagtt ggatttgatt ggcaagataa aaaacaggtt
    53821 tgggaaaaag ttatggaaga gctaggggaa tttttgaagg agaaaaataa ggaaaggaaa
    53881 ttcagagagt ttggtgatct cttattcagt ttaattaatt actccaggtt tgaagatatt
    53941 aatccggaag aatctctgga aaggaccaac caaaaattta ttaaacgatt ttcttatata
    54001 gaagaccgga tcagggaaaa gggtaaaaat atttctgaag ctaccttgtc tgaaatggat
    54061 aaatattgga acgaatcaaa aaaattcaac tgataattac caaccaattt actcagaaat
    54121 ctgtaggtgc ataccagggt tatactcagt ttactttgtt ttacaagaag attgcagaac
    54181 caatgaccat gttgggaatg gcatctgggc gagagaaata tttttttttc gaagtttgaa
    54241 gtctggtgat tatctatctt tgcgaccatg aatgcatccg aagacaatct gctgaaaaaa
    54301 gttattgccc actccaaaga gtacggattc atttttcctt ccagtgaaat ttatgatggc
    54361 ctgggtgcag tttatgatta cggtcagaac ggggctttac taaaaaataa tataaaagaa
    54421 tactggtggc gagcgatggt gcagctccat gagaatattg ttggcattga ttcggcaatt
    54481 tttatgcacc ctaccgtgtg gaaagcctcc ggtcatgtgg aagcttttaa tgacccctta
    54541 attgataata aagattcaaa gaaaagatac aaggcggata atcttgttga agatcatata
    54601 gaaaaaatcc ggggaaagat tagcaaagaa attgaaaagg cggaaaaaag attcggcgcc
    54661 gaattcaata aggaaatgtt ccttgaaacc aatccccgcg ttaaagaata cagcgagaag
    54721 attaaaacaa ttgaagaccg tcttagaggg gctttgggaa gcggcgacct ggcgggattg
    54781 aagaaactta tcgatgatct cgaaattaag gatcctgttt ccggttcagg aaactggacg
    54841 gaagtgagac aatttaatct gatgttcgca accgatatgg gctcggtagc cggtgaatct
    54901 aataagattt acctgcgccc tgaaacagcc caggggattt ttgtcaattt tctgaatgtg
    54961 cagaaatcag gcaggatgaa aattcccttt ggaatagccc aaattggaaa ggccttcaga
    55021 aatgaaatag tcgcccgtca gttcattttc agaatgcgtg agtttgaaca aatggaaatg
    55081 caattcttcg tgaagccagg agaggaaatg aagtggtatg aattctggaa gaagaaaaga
    55141 attcaatggc ataagaacat gggaatttca caagaaaaat atcgcttcca taatcatatt
    55201 aacctcgccc attacgcgaa tgcagcttgc gatattgagt tcgaatttcc tatgggattt
    55261 aaggaactgg aaggtatcca ctccagaaca gattttgatt taaaagcgca tgaagaacat
    55321 tcaggaaaaa aactccagta ttttgatcag gaagaaaacc ggtcatatgt tccctttgtg
    55381 attgaaacct ccatcggcct tgacagaatg tttctggcta tattgagctc atcttataaa
    55441 gaagaaaaac tggaagatgg cagcgaacgg gttgtgatga atatacccat gttcttatct
    55501 ccggtacagg tggcaattct accccttgta aaaaaagacg gactgcccga gattgccacc
    55561 gaaattatga atgaacttaa atatagtttt caatgtactt atgaagagaa agataccata
    55621 ggccggagat atcggcggca ggatgccatc ggtacgcccc tgtgtgttac gattgatcaa
    55681 caaaccaaac aggatcaaac tgtcaccatt cgtttcaggg attccatgga acagaaaaga
    55741 attaaaatcg gtgaattgaa ttcggtaatt tcagaactgg tttccctaaa atcttttctc
    55801 agaaaattat gacgcggtcc accaaactct gaaaaacttt gtgtccttgt gcctttgtgg
    55861 ttaactaaaa ttctgaaaaa actttgtgtc cttgtgcttt tgtggttaac taaaatcctg
    55921 aaaaactttt taccctcgta gctaaaaaat acttagcgga atattaaata attttctata
    55981 ttgggcacta gataggtcaa aagatagatc aatagattta gctaaacccc gaaagttaaa
    56041 tctactacaa tgaaagaaag ctactcccca agagcagctt tacctttagt tttctgcaca
    56101 ttcatttttt catgctcagt ccttcaggag aatcgcatca atgaagaact cctggataat
    56161 tttccggttc cagacggacg gagttacggc ttggtaatca gttacgaagt cgaatcgctg
    56221 tcgttctcgg aattttcagg tggagatccg ccgcaggagc tggcgctgca gtctgaaccc
    56281 gcggtttcac gaagtaaaat caaaacagtg atttatcagg acggaagctc cgactggttg
    56341 attaaaaatg taaaaccgcg taatcctccg gttgaatttc ataacatacc taaaagtgat
    56401 tacgatgaga tatccacaat ccgaattatc aataaccaag ggtatttcat aaatggaaat
    56461 ggagatttaa ttcataagca aagaatcgat gttccctctt ttgctgagtt aattctggat
    56521 gtacaaaatt ccaacaataa aaacataaat gccagggttt cagaggtgtt aagtggaaca
    56581 atatttcttt cggaacaaag gctttctgaa ctaagggaaa tcaacctggt tgaagaatta
    56641 ccttctggtc ttattaaaat taaggatctt gaattatata ataaaaactt taatagcagg
    56701 acgcaggagg aagaattgat ctatgaaact tatggtgacc ccataaccgg attagtggtt
    56761 ggaggcaagg tgcttaatca aaatggggaa ctgatttctt atgttgtata taaatatagt
    56821 gaagcgggca tgggttctat cctggagatg attcactaca aagagtttca ttctggtgac
    56881 gatgaaggta ctgaagttgg catattgaat aattacttcg aaaatattgt gatcgaaaac
    56941 catttaaatt aaaacctcat gaagattcta agaataattt tatgttttgc aataacattt
    57001 ctggcaaacc agattagcca aagtcaagac agaccggtta tctgggtgca tggccttaac
    57061 gataataaca ctttttgggc taatatggcc actgttttca atacagaaag aaggatgaca
    57121 tcttctaatc taaattatgg cactggtaat ggggttgtaa attttgccac acagattcgc
    57181 aataataata tagcaagaac gagcaacatt gttatcggtc atagcatggg tggtgttgcc
    57241 cttagggaag ttaatagggt gaattctaac ttctttggcg gagcaattac ctttggttcc
    57301 cctttagatg gagccagaat agtgaattcc tgggctaatg gatcgtttgg ttcatttata
    57361 caaaaatcta caaaagagct tactgccggg cctctcgctg aatacggcgt ggacgttaag
    57421 ttattagatt atctgggacg aaaatggttg ggagctacgc tttctttaat tccccgggat
    57481 tttattatga atcaatttaa caatttaatg accggtcaat cagccgctga tttggcagaa
    57541 aacagtccct attattccgg agtgaaaaat ttctatagcg gtattccaaa aataaatgtt
    57601 tttggaaacg aaaacgcccc tgcaatgcag agattagcat cgatcagtct gagcaacgga
    57661 caaaatgaca cggatgtgat agggctgatt agctctgcaa aatcagtata tacagccttc
    57721 attaacataa ataataccag agcgttatta aaccccttgc atgctccgat ctattatgcc
    57781 cgttcttatc agtggaaaag aggcctggat ttttttaata aaagtctgga acccggctgg
    57841 gcgggtctta taggctctac ccgaaccgaa acaaccacat atacctacga aacttatgta
    57901 tgtgatgagg atgaattcaa ttataacaat caattggagc gaattgaaac tgattgttgg
    57961 caaacagtta ctaaaactta cacaaaaact atcattgaac ccagcgacgg cttaattcac
    58021 aaaagctcac agatcggagc gaaatcatca tggaagggta cagcggtaga agcgctcgga
    58081 gtgaatcatc tggagatggg gagacattgg gagtcggaga gaatattacg ccaaattttt
    58141 gatggatcaa tccatcctaa tatttcaata cccaggagat gaacaataat ttttatttac
    58201 gactactttt tcttactgca atactaatta gttgcagaga tcatttcgaa attgaaattg
    58261 atcaaaatgg cgctgttcta aaaaccagtc atctttggac taaacactta gtaaatgaag
    58321 gtgatgaatt gtttaccttc attactccgt ttgaacttgg agagacagta actttaatgg
    58381 gcgctgattt caaggaacag gctttacgaa aattggacat aaataatgga aatcaaatct
    58441 gggagtggaa tgaattcaat tttgaagacc gatgggatgt gtattattat cattctttcg
    58501 ataataaatt gattgttcca aatggtgcaa atttttattc aatagatata aaaacaggcc
    58561 agaccatttg gaaagaaaaa ctcccctatc aggtgaaccg ctctgttgcc ggctacaggg
    58621 aaaattattt tttctccgga tacctgtacg aaattgaaac ccggcccaca attttcaaag
    58681 gcaacctata tacgggagaa tatgctccat ttctt
//
LOCUS       DASWHX010000045         8891 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_3355009, whole genome shotgun sequence.
ACCESSION   DASWHX010000045 DASWHX010000000
VERSION     DASWHX010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 8891)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8891)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8891
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3355009"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(516..845)
                     /locus_tag="VGA21_02795"
     CDS             complement(516..845)
                     /locus_tag="VGA21_02795"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018472844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HEX9649465.1"
                     /translation="MKQENFFEDVYEVVKLIPKGRATSYGAIAQYLGLKSSARMVGWA
                     MNSAHDRDDVPAHRVVNRDGLLTGRNHFATPTRMEKLLKKEGIRVKNNQIFDFQKVFW
                     DPMQELM"
     gene            complement(858..1796)
                     /locus_tag="VGA21_02800"
     CDS             complement(858..1796)
                     /locus_tag="VGA21_02800"
                     /inference="COORDINATES: protein motif:HMM:NF025598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HEX9649466.1"
                     /translation="MSKINNKILIVVLLVLAGAYLGNKYLGSKGERNFKNVLVDLDTA
                     LIDRILLTPPPSKGGEVLINKQSPGKFTVTKDNLIDEGDIGMIRSMLSAHIEMKPERL
                     VSNSSDKWAEYEVSDSTGVNVKMYSGTKKLSEIIVGKFDFQQATRTMSTMVRISNEEQ
                     VYAVDGFLSSIFNVEFDALRDKTFLKANNDEIQLVKLDYPADSSFSLALENGEWQLDG
                     QPADSASCVKFTNGLMFLNLRDFVNDFEPAKNELLYKLTIEGDSISTIIVDCYKQSDQ
                     YILHSTLNENAYFNPGVNNIFEKLFPPKSKFYPNPE"
     gene            complement(1789..3309)
                     /locus_tag="VGA21_02805"
     CDS             complement(1789..3309)
                     /locus_tag="VGA21_02805"
                     /inference="COORDINATES: protein motif:HMM:NF021351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HEX9649467.1"
                     /translation="MRTKKSYYTTLTLVIAIIVFINFLSADFFARLDLTENGRYTLSK
                     ATKDIISNLDEPITIRAYFTENLPPNIAQTRKDFRDMLIEYSNRSDNNVVYEFINPNE
                     SQKVEQEANQAGISPVMINVREKDQMKQQKAYLGAIVQKGNQQEVIPFLQPGEAMEYA
                     LTTSIKKLSVQNKPSIGLIQGHGEPGVQGMQQAGFELGVLYNFEDHVMTDTTVIPSKF
                     RTIAIISPQDTIPQSHFVQIDNFIRQGGRILVGIDRVNGDLQNAYGSSMSTGLESWLA
                     AKGINVENKFLIDASCAAVNVQQQQGSFRFNTQIQFPYLPVINTFSDHAITKGLEGVL
                     LPFASPLTFSGDSSVQFTPIAFSSAKSGTAPVPAYFDIQKQWQESDFPLEELIVGATL
                     EGTISGNPASKMVVIGDGDFPINSRSGGQGQPLQRDNVSLFVNSIDWLSDDTGLIELR
                     TKGVTFRPIDQITDSTKTLLKYVNFLLPIILIIAYGLIRMQIKRSLRFKRMEMSYE"
     gene            complement(3313..4038)
                     /locus_tag="VGA21_02810"
     CDS             complement(3313..4038)
                     /locus_tag="VGA21_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002705894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX9649468.1"
                     /translation="MRPIWIIAKKELQSFFDSLIAYIMIVLFLGFSGFFTWLYGSDVF
                     LVGQANLNGFFSIAFWTLFFFIPAITMRTLAEERKTGTIELLLTKAVTDRQVILGKFL
                     ASLLLVVIALAFTLPYYITLASIGSVDHGAILTGYLGLIFLSAAYISIGLFTSSLTGN
                     QIVAFLLALFIGLFFHLIFDVLAGNFTGFTGQLFTTLSLTSHFDSMSRGVLDSKDIIY
                     FLSIIVIGVFLSELNLAKRNLAN"
     gene            complement(4051..4974)
                     /locus_tag="VGA21_02815"
     CDS             complement(4051..4974)
                     /locus_tag="VGA21_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002705893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX9649469.1"
                     /translation="MDIKIENLTKNYGPQVAVDDISFEVKAGEILGFLGPNGAGKTTT
                     MKMITNYIAIDHGDILLGGKSILNEGDTLRRHIGYLPEHNPLYHDMPVIDYLKFSAAL
                     QGVYKSEITDRIKEMVKTCGLNAEKHKKIGELSKGYRQRVGLAQAMIHNPDVLILDEP
                     TSGLDPNQIVEIRKLIREIGKEKTVILSTHILPEVEATCDRILIINKGRIVADGTAET
                     LKKQAHGQELIITTIDEGNPADIKNALLELSAVASVTNIDTNRFEIQSKPDKHSAREV
                     FQLCVGKGWVITELTPTQTKLEDIFRNLTTN"
     gene            5229..5666
                     /locus_tag="VGA21_02820"
     CDS             5229..5666
                     /locus_tag="VGA21_02820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649470.1"
                     /translation="MKTGTIKFLIAISLIASAISCKNKNKISPQDAAKTQISKTWILD
                     TGTAVTLEGEDVTADYFFFEITFTAAGTYATQNGIPAFNANGTWSFAGESLTQINRDT
                     DISTTITLTDNDLLMTFTLSESQLGSRTTSLAGEYIFKLVKKN"
     gene            5869..6762
                     /locus_tag="VGA21_02825"
     CDS             5869..6762
                     /locus_tag="VGA21_02825"
                     /inference="COORDINATES: protein motif:HMM:NF023805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M57 family metalloprotease"
                     /protein_id="HEX9649471.1"
                     /translation="MKNFRRLIILVLFGIILFSCNIEEVEKTEFVFETSRVLEDYEMP
                     NEDIELLQQLGFAGSSGIVMEKTQIWNGGATYIYYLMEGDIEIRKDLLKSMVDELPKN
                     GKVSQYRTANLVGGPFPRTIRVIGMNMNNTTLQQGLTMAIQNYNNENINLNFTLEFRT
                     VTNIWQSISAVNDSDILAQQYSGSAGGNSGFPSGGNPYANVNVSTSTASFGTNVCENV
                     FTHEIGHTIGLRHTDFFNRSISCGGSFNNEGDTGVGAIHIPGTPATTSIDMNSIMLSC
                     FNAGVTGEFSNFDVTALEGIY"
     gene            6772..7140
                     /locus_tag="VGA21_02830"
     CDS             6772..7140
                     /locus_tag="VGA21_02830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649472.1"
                     /translation="MKRKSIIIVLVSLLFACKNEELTELDKNTKVITENAQIGKGNVY
                     TYTIGTSIPTEGDAIISKQANYFDVSEITTGASGVIYQYKPKPDFLGKDNVELIQTFS
                     SGGAVFGRNIIKISITVATD"
     gene            complement(7277..7849)
                     /locus_tag="VGA21_02835"
     CDS             complement(7277..7849)
                     /locus_tag="VGA21_02835"
                     /inference="COORDINATES: protein motif:HMM:NF039496.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5655 domain-containing protein"
                     /protein_id="HEX9649473.1"
                     /translation="MAKTSQQIEQEFIEGLKLSTGKDLKTWLSMVKKQGMEKRNDMIN
                     WLKKDLGFGHMNASLLIGIYLNDGKPVYANADNLLKDQFEKKEHLKPLYDQLIKEVQK
                     EIKDTQVTAKKTYVSLHKKKEFAAINIKSNELRVGMDLGDTPFSDYLQKSKLTGPMPR
                     ISHMVIIQDKKDINSKLLGLIQKADKRING"
     gene            8156..8240
                     /locus_tag="VGA21_02840"
     tRNA            8156..8240
                     /locus_tag="VGA21_02840"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8190..8192,aa:Ser,seq:tga)
     gene            complement(8486..>8891)
                     /locus_tag="VGA21_02845"
     CDS             complement(8486..>8891)
                     /locus_tag="VGA21_02845"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEX9649474.1"
                     /translation="VALDWTFKMGLVTDDFLIYLLLITRLIVMFIIFQIGISAIYYFA
                     PAVKYKWGFFSTGSIIATILSLAISYGFSYYISQFGTYNKFYGSIGALIALMIWFFFL
                     SVIMLVGYEVNASIHKVQMEVKDLEDGKVAVV"
BASE COUNT         2725 a         1716 c         1688 g         2762 t
ORIGIN      
        1 gcatgagcgc attgaatata aagaatcctg aaaagtttga gagagttcag tatatgaaag
       61 cgctaagtac atattgtgtt tgaagccgca aagagatagg agtaaaaaca catttttatt
      121 aatacatatc tgttttccgc gaaatctgcg ggagaaaaag gagtttattc tgttcaaatt
      181 tcaaccgcag aaaaaaagcg aaatactttt tttattaatc cagctgatat caaagatatt
      241 gcagaattga ttcactgagt tttctgcgca aatccgcata atctgcagga gaaaggaaat
      301 taaacatccc tcgaaatacg ctgattaccg ctgataattc tggatgtttt ttgacggaat
      361 gtatatttta ttttctgcgc aaatccgcga aaactgctgg agaaaagaaa ttaaacatcc
      421 gcagatcatc cgcagaattg attcactgag tcttctgcgc aaatccgtga aatccgcggg
      481 agaaaaagaa ttatatatgc ctcatggaat ttcatttaca tcagttcctg catcggatcc
      541 caaaacactt tttggaaatc aaaaatctga ttatttttaa ctctgattcc ttcttttttc
      601 aaaagctttt ccattcgcgt cggtgtggca aagtgattcc tgccagttaa taatccatca
      661 cgatttacaa cccggtgtgc aggtacatcg tccctgtcat gcgctgaatt cattgcccag
      721 cctaccattc ttgcactgct tttcaaccca agatattgcg caatggctcc ataactggtt
      781 gctcgtcctt tcgggataag tttcacaact tcatatacat cctcaaaaaa attctcctgc
      841 ttcattacag gatgaattta ttcgggattt ggataaaact tgcttttagg tgggaataat
      901 ttttcgaaaa tgttatttac tccagggttg aagtaagcat tttcgttcag cgttgaatgg
      961 agaatatact gatctgactg tttatagcaa tcaacaatta ttgtactaat actatctcct
     1021 tcaatggtca gcttatatag cagctcattt ttagcgggtt caaaatcatt tacgaaatct
     1081 ctaagattca ggaacatcaa cccgtttgta aattttacgc aggatgcgga atcagccggc
     1141 tgaccatcca attgccattc accattttcc agtgctaaag aaaaactgga gtctgcagga
     1201 taatcgagct tcaccaattg aatctcatca ttgtttgctt tcagaaaagt tttatcccgg
     1261 agggcatcga attcaacatt aaatattgaa gataaaaaac catccacagc atatacttgc
     1321 tcttcgttgg aaatccttac cattgtagac atagttcggg ttgcttgttg aaagtcgaat
     1381 ttcccgacaa taatttctga caactttttg gtgcccgaat acatcttcac attcactccg
     1441 gtagagtcac tcacttcata ctccgcccat ttatcagacg agttggaaac caggcgttcg
     1501 ggcttcatct caatatgagc tgaaagcata cttcgtatca ttccaatatc accttcatca
     1561 ataaggttgt ctttggttac tgtaaatttt cccggtgatt gtttgttaat aaggacttca
     1621 cctcctttgg aaggtggtgg ggtaagcaga atccggtcaa tgagagcggt atccagatca
     1681 acaagaacgt ttttgaaatt tctttctcct ttgctgccca agtatttatt ccctaaataa
     1741 gctcccgcca gcactagtaa tactactatc aaaattttgt tattaatctt actcatagct
     1801 catttccatt cttttgaacc gtaaacttct tttgatttgc atcctgatta aaccataagc
     1861 aattattaaa attataggta acaggaagtt cacatatttt aataatgtct ttgtgctatc
     1921 agtaatctga tctatgggcc tgaaagtgac gcctttggtc cttagctcga tcagcccggt
     1981 atcatctgag agccagtcaa tactgttcac aaacaggctc acattgtctc tttgaagcgg
     2041 ctgcccctgc ccgcctgagc gggaattaat gggaaaatcg ccatctccta ttaccaccat
     2101 tttggatgcc ggattgccgc taatagttcc ttctagtgtg gctccaacta tcaattcttc
     2161 caggggaaag tcactttcct gccattgttt ctggatatca aaatatgcag gaacaggagc
     2221 ggtccccgat ttcgccgaac taaaagcaat aggggtgaat tgtacagatg agtctccact
     2281 gaatgtaagc gggctggcaa aaggtaaaag tactccttct aaaccttttg taatggcatg
     2341 atcagagaat gtgtttataa ctggcaaata tggaaactgg atttgtgtgt taaacctgaa
     2401 agatccttgc tgctgttgaa cgttaacagc tgcacaactg gcatcaatca gaaatttgtt
     2461 ttccacattg attcctttgg cggctagcca gctttccaat ccggtactca tagaactacc
     2521 ataggcattt tgtaaatctc cgtttacgcg gtctattcca accagtatcc gacccccctg
     2581 ccggataaaa ttgtcaatct gcacaaaatg actttgcgga atagtatcct gcggtgagat
     2641 aatcgcaatc gttcggaatt ttgaggggat aacggtggta tcagtcatta catggtcttc
     2701 aaaattataa agaactccca attcaaaacc cgcctgttgc atgccttgta cgccaggttc
     2761 accatgccct tgaattaagc caatggaagg tttgttttga actgataatt tcttgatact
     2821 ggtagtcagg gcatattcca tggcttcccc aggctgtaaa aaaggaataa cttcctgctg
     2881 gtttcctttc tgcacaatcg ccccgaggta tgccttttgc tgcttcatct gatctttttc
     2941 ccgtacattg atcattaccg ggctaatacc tgcctggtta gcctcttgtt ctaccttctg
     3001 actctcattt ggattgataa attcatatac cacattatta tcggagcggt tagaatactc
     3061 gatcaacatg tcccggaaat cctttcgtgt ctgtgcaatg tttggcggaa ggttctcagt
     3121 gaagtaagcc cttatagtta tcggttcatc aagattgcta ataatatctt tggtcgcttt
     3181 ggacaaagtg tacctgccat tttcggtcaa atccagccgc gcgaaaaaat ccgcagaaag
     3241 gaaattgata aaaacgataa tggcaatcac cagggttaaa gttgtgtagt atgatttctt
     3301 ggttctcatt tcctaattgg ctaagtttct tttggcaagg tttaattcag atagaaaaac
     3361 tcctatcaca attatgctca gaaaatagat aatatccttt gagtcaagaa caccccttga
     3421 catggaatca aaatgggaag tcaagcttaa ggttgtgaac aattgtccag tgaaaccagt
     3481 aaaatttccc gccaatacat caaatatcag gtggaaaaaa agacctataa aaagtgctaa
     3541 taagaaagca acaatttgat ttccggttaa actactggta aataacccaa tgcttatgta
     3601 tgccgcactc agaaatatta gtcccagata acctgtgaga atagcaccgt gatctactga
     3661 gccaatgctg gctagtgtta tatagtatgg gagggtgaag gcaagggcta taactaccag
     3721 cagaagactt gcgaggaatt taccaaggat aacttgccta tcggttaccg ccttggttaa
     3781 taataattct atggttccgg ttttcctttc ttcagccagg gttcgcatgg ttatggcagg
     3841 tatgaagaag aacaatgtcc aaaaggcaat gctaaaaaaa ccattcaggt tggcttgccc
     3901 caccaggaat acatccgaac cataaagcca ggtaaaaaat ccgctgaagc caaggaacaa
     3961 aactatcata atgtatgcga ttagtgaatc aaaaaatgat tgaagttctt tttttgctat
     4021 tatccaaatc ggtctcattt attcattctt ttaattagta gtaagatttc tgaaaatatc
     4081 ttcaagtttg gtttgggttg gggtaagctc ggtaattacc catccctttc ccacgcataa
     4141 ttgaaatact tccctggcag aatgcttatc cggtttactt tgaatttcaa accggtttgt
     4201 atcaatattt gtaactgacg caaccgcact caactcaagc aatgcatttt ttatatccgc
     4261 aggattgccc tcatcaattg ttgtgattat taattcctgt ccatgcgctt gtttctttag
     4321 ggtttcagca gtaccatctg ccactattct tcctttgtta atgatgagaa tcctgtcaca
     4381 agtggcttcc acctcaggaa gaatatgggt gctgagaatt actgtctttt cttttccaat
     4441 ttccctgatt aattttctga tctccacaat ctggttagga tcaagtccgg aggtcggttc
     4501 atccagaatg agaacatcgg gattatgtat catggcctgg gctaatccaa cgcgctgccg
     4561 gtatcccttt gataactctc caattttctt gtgtttttcc gcattcagac cacaggtctt
     4621 taccatttcc ttaatgcggt ctgtaatttc acttttataa actccctgca gagcagcaga
     4681 aaatttcagg tagtcaataa caggcatatc atggtagagt ggattatgct ccggcaggta
     4741 tcctatatgc ctgcggagcg tgtctccttc attgagaatg gacttccccc ccaaaagaat
     4801 atcaccgtgg tcaatggcta tataattggt aatcattttc atagttgtgg tctttcctgc
     4861 tccgttaggg ccaagaaacc caaggatttc accagccttt acttcaaagg agatatcatc
     4921 cacggccacc tgtggaccat agttcttggt taaattttca atctttatat ccataagtta
     4981 tggcattttt gtcgggcaca ctactcctca aaatgggccg taaaagtaag taaaatgatt
     5041 agaaaatgtg aacctctttt tcaggttttt aacatttttt aagaatcatt ggtgtaatcg
     5101 gggcaaaaat tgttattgaa gccggacgcc ataggacatt tgatacctat ataccagtct
     5161 ttgggagatt tgtaccagtt ctcttgcctt cacacttgct aaatttgttg ctcaaataac
     5221 aaaaaattat gaagactgga acaataaaat ttctgattgc gatttctctg atagcttcgg
     5281 ccatttcttg taaaaataaa aataagataa gtccacaaga tgccgctaaa acccagattt
     5341 caaagacctg gattctggat acaggcacag cagttaccct ggagggcgaa gatgtaaccg
     5401 ctgattattt cttttttgaa attacattca ccgcagccgg tacctatgcc actcaaaacg
     5461 gaatcccggc gttcaatgca aatggaacct ggagttttgc aggggaaagt ctaacccaga
     5521 ttaacaggga tactgatatt tcaacaacaa ttacactcac tgataatgac ttgcttatga
     5581 cattcacttt gagcgaatca caattgggaa gccgcaccac ttcccttgca ggagagtata
     5641 ttttcaagtt agtgaagaaa aattaataaa tcttccgttg cggagtaacg gcttgttata
     5701 ggcaataaaa tgtgctattt attaacgttc agagtgtttt cctactctga caacctgttt
     5761 tattacggaa aaaatctaaa aggaaagcac actttatgcc attacaggag aagtatttga
     5821 cttgaacact ccttaattaa agtagagcga aactaaaact tagaaaaaat gaaaaacttt
     5881 agaagattaa taatcttagt gctatttgga ataatattat tctcatgtaa tattgaagag
     5941 gttgaaaaaa ccgaattcgt atttgaaaca tctagggtac ttgaggacta tgaaatgcca
     6001 aatgaagata tcgaattact ccaacagtta gggtttgctg gtagttcagg tattgttatg
     6061 gaaaaaaccc aaatttggaa tgggggagct acctatattt attatctaat ggaaggggat
     6121 attgaaatta gaaaagactt gcttaaatcg atggttgatg aattaccaaa aaatggaaaa
     6181 gttagccaat atagaacagc gaatttagtt ggagggccat ttccaagaac aataagagtt
     6241 attggtatga atatgaacaa tactacacta cagcagggct taactatggc aattcagaat
     6301 tataataatg aaaacataaa tttaaatttc actttggaat ttagaacagt tactaatatc
     6361 tggcaaagta tttcagccgt aaatgatagt gacattttgg cccaacaata tagtggatct
     6421 gctggtggca attctggatt tccatcggga ggaaatcctt atgcaaatgt caatgtttct
     6481 acttcgacag caagttttgg aacgaatgta tgtgaaaacg tttttaccca tgagatagga
     6541 catacaatcg gattaagaca tactgacttt ttcaatcgtt ctatttcatg tggtggttct
     6601 ttcaataatg aaggggatac tggcgttggt gctattcata ttcctggtac ccctgctact
     6661 actagtattg atatgaattc aataatgtta tcttgcttta atgcaggagt tactggagaa
     6721 tttagtaact ttgatgtaac cgcacttgaa ggaatttatt aataaaatat tatgaaacgc
     6781 aaatcaatta ttatcgtact ggtatcattg ttatttgctt gtaaaaatga ggaattgaca
     6841 gaactcgata aaaacacaaa agttattact gaaaatgccc aaattggcaa aggaaatgtt
     6901 tatacatata caattggaac atcgatccct actgaaggag atgcaataat atcaaaacag
     6961 gcaaattact ttgatgttag tgaaattaca acaggggcat ctggtgtaat ttatcaatat
     7021 aagcctaaac cagatttcct tggaaaagac aatgttgaat taatacaaac atttagtagt
     7081 ggtggggctg tatttggcag aaatatcatt aagatttcta ttacggtagc aacggattaa
     7141 taaagcccat aacactgtat aagaaaacat aggttgagta gtggggaaag aaatttcatc
     7201 actactttat tatttcagag caattgggaa aagacatcaa tttctgccac ctacgtttct
     7261 tatactcacc aaagtcttag ccgtttatcc gtttatcggc tttctgaatt aatcccagca
     7321 gcttagaatt gatgtctttt ttgtcctgaa taattaccat atgggagatt ctgggcatag
     7381 gtcctgtgag ttttgatttt tgaagatagt cgctaaaggg tgtgtcacca agatccattc
     7441 ccactcgcag ttcattggat ttaatattga tggctgcaaa ttcttttttc ttatgcagcg
     7501 atacataggt tttcttggca gtaacctgag tatctttaat ttctttctga acttccttta
     7561 tgagctggtc atagaggggc ttcagatgtt ctttcttttc aaactgatct tttaacaggt
     7621 tgtctgcatt cgcataaacc ggttttccat cattaagata aattccaatc aggagggaag
     7681 cattcatgtg accgaatccc aggtctttct tcagccagtt aatcatgtcg tttctctttt
     7741 ccatgccctg ctttttaacc attgaaagcc aggtcttgag atccttacct gttgaaagtt
     7801 ttaatccttc gataaactcc tgttcaattt gttgtgatgt ttttgccata ataagattta
     7861 tttattactc taaaatgttg aatagcaatt tcagggaatc ctgctctaat gtattggaaa
     7921 aaatttacca ggagcagagg ctggcaggtc aattccaaaa ttaggaatca tatctattcg
     7981 agttaaggta tcgtgtgaag gaataatatg attaaggttg aaaatagtgt gaacctcata
     8041 aactatttaa atacaaagtt tcccgtgcga tccaaaacta accgttatat caaaatttga
     8101 aggaaaaagt tgtctttgtt aaaacggaaa aatcagatta cttttgccgt cttacagagg
     8161 agtggcagag cggtcgaatg cggcggtctt gaaaaccgtt gaaccgagag gttccggggg
     8221 ttcgaatccc tcctcctctg caaatagcaa aagaatccct cctcaagatt tagtagaatc
     8281 cggagttaat tttttgaaat ttatttagtc acagatttac tgatcccttg gtgataaacc
     8341 aggaactttt cagttttacg ctgatgtcac ctgataaatt gattttgaac cattattttt
     8401 atttttgaac agagttttaa ttagataaca acaaacccaa ttcgctaaac gaatttatta
     8461 aagagtaaag tacaaggata tagttttaca caactgcaac cttcccatcc tccaaatctt
     8521 taacttccat ctgtactttg tggatactgg cattaacttc ataaccaacc agcataatta
     8581 ctgaaaggaa gaaaaaccag atcataagag caattaatgc tccaatagaa ccgtaaaatt
     8641 tattgtacgt tccgaattga cttatatagt aagaaaagcc ataggaaatt gccagggaaa
     8701 gtatggttgc aatgatggag cctgtgctga aaaaacccca cttatattta actgcaggtg
     8761 caaaataata aatagccgaa attccaatct gaaaaatgat aaacataacg ataaggcggg
     8821 taataaggag aagataaatg aggaagtcat ctgtaaccag gcccatttta aatgtccagt
     8881 caagggctac t
//
LOCUS       DASWHX010000046        83667 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_3355364, whole genome shotgun sequence.
ACCESSION   DASWHX010000046 DASWHX010000000
VERSION     DASWHX010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 83667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 83667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..83667
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3355364"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            119..1000
                     /locus_tag="VGA21_02850"
     CDS             119..1000
                     /locus_tag="VGA21_02850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649475.1"
                     /translation="MNKGNKVRYVTNTKIDSLISLIDTLIGTIDSIGKSFEGKSESEL
                     GILKLVYLDILKRTVPNLHSICILLGQYKAYNQYKISIFLLLRTCLSDSLLGLYLSTY
                     RMDKNSFENEVNVIDRSYFVAMKKFLKEEPRYKIPVASDTDLTKEFENNFSNLRKGHP
                     EFFKKDKSLKSNKELRSRSKKELFAIKRGEKPDMSYSPTDENFYQRLIKFRALKAYGY
                     IYLLIKFFAQYHHYAFKTRSLIESNREFEFNKIVLSISFLYATTDTFGNLLDLDKKLT
                     APLKKRIEEFLTFKTNS"
     gene            complement(1117..1323)
                     /locus_tag="VGA21_02855"
     CDS             complement(1117..1323)
                     /locus_tag="VGA21_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011586287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX9649476.1"
                     /translation="MAKRAINRIKIVLVEQGRTNKWLADKLGKNATTVSRWCTNDMQP
                     SLETLVQISNVLNVNVRELLVSTK"
     gene            1642..1839
                     /locus_tag="VGA21_02860"
     CDS             1642..1839
                     /locus_tag="VGA21_02860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649477.1"
                     /translation="MRYVDLPESEFQKLLQQIEEDCDEYGDDTPLLTLQQVILEELRQ
                     KLEEDKMKWKKFPVWNLFKRQ"
     gene            complement(2153..2794)
                     /locus_tag="VGA21_02865"
     CDS             complement(2153..2794)
                     /locus_tag="VGA21_02865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649478.1"
                     /translation="MRKIITILTFLTVSVSGYGQTIEDFFTGHNYPVAWLGIDFSQVQ
                     LVGDFSHFGGHGPKDPGDLQIDYFPSWNRLLMNEPDKYNLKEFFRRPDLEININMVMA
                     RNAKADTDNMLTYSPKYFRKDDIRQIIESYDLEGEVGIGIVFIAESLNKIDTEAYFHV
                     IVLNMTNKEVLLSERLRGQPSGFGIRNYWAGAIFEILKNIDTRYYRLWRSKYM"
     gene            2915..3262
                     /locus_tag="VGA21_02870"
     CDS             2915..3262
                     /locus_tag="VGA21_02870"
                     /inference="COORDINATES: protein motif:HMM:NF021182.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HEX9649479.1"
                     /translation="MEQFKKRDIKLKGWDTENNLLMRLNSIDCKKGLLYKEHFLLLQY
                     TGLLDKSENEVYDGDILLIASKKFLVFWDSERSGWGLAENPQLKNKKPLLARDVSKST
                     RLCSFLESPQSFG"
     gene            complement(3562..3798)
                     /locus_tag="VGA21_02875"
     CDS             complement(3562..3798)
                     /locus_tag="VGA21_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020530212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEX9649480.1"
                     /translation="MLDVRVKVTEILVEKLGIAPTEVTPDANLIKDLGIDSLDYAELV
                     MEFEQAFNIRIPDNDAEQLQTVNEAVKYIEKKLG"
     gene            complement(3867..5213)
                     /locus_tag="VGA21_02880"
     CDS             complement(3867..5213)
                     /locus_tag="VGA21_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009578264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9649481.1"
                     /translation="MDSKKFKSTVIIRILLIITTSILLYETLGGDNIFLTPILFLFLL
                     VIQLISLIKFLDKKNSEIASFLDSIEFDDDTASFTTSEDAEPLNKINQEFNKIILKFK
                     SVRREKEADYQYMKNIVQHVGIGLITFEKDSGQIQIMNAAAKKVLRVSNLVNIRELKS
                     LNENLVDIILRLKTGGRDLIKLEVGGDLIQLAVYAIELNLRGKEYKLVSLQNIHSELE
                     EKEMEAWQNLVRVLTHEIMNSITPIQSLAATVESEIESQIKTSKLNGEQFEDMQLAVQ
                     TIQKRSQGLIRFIQDFRNLTHTPKPKLKTIKVTQMLDEIGTLMQKELDQNKIYFIKSL
                     QPADLKINADKELIEQVLINLIKNSIQAFDEQNERIIEMRAYKTDKGRPVISVKDNGS
                     GIDEEALQRIFIPFYTTKKSGSGIGLSLSRQIMRQHQGSITVKSKLDEGTEFSLKF"
     gene            complement(5203..6582)
                     /locus_tag="VGA21_02885"
     CDS             complement(5203..6582)
                     /locus_tag="VGA21_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019597951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX9649482.1"
                     /translation="MEEKALGKILIIDDDEDVLLAAKLLLKKHAHQVIIEKNPKKIPF
                     LLNNDTYDVILLDMNFSKDTTSGKEGFYWLKEITHKDPNAVVVLITAFGDVEMAVKAL
                     KEGATDFILKPWQNEKLLATLSSAIKLRQSYREVDQLRKAKKQLEEDINQPFKDILGN
                     SSAIKKIFDLIEKVAATEANVLILGENGTGKELVARAIHQRSLRKDNVFIGVDMGAVT
                     ETLFESELFGHKRGSFTDAKEDRPGRFEIANKGTLFLDEIGNLSLPLQSKLLSVLQKR
                     EIIRVGTNQPVSIDIRLICATNLPILEMVKENKFRQDLLYRINTVEIHLPPLRDREED
                     IPMLAEHFIQNYGKKYRKPGIRISESAIKKLQKYSWPGNVRELQHAIERAVIMSDGDT
                     INPDAFFFLNHAQINTTDQSDNYNLDEVEKQVILKAINKFDGNISKAAKELGLTRASL
                     YRRLEKHGL"
     gene            7024..7683
                     /locus_tag="VGA21_02890"
     CDS             7024..7683
                     /locus_tag="VGA21_02890"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX9649483.1"
                     /translation="MIITSTTEQKNPNYHPIKLALIQHDILQRKLFKRLLESLQRFQE
                     IRSYSTCEELIKLSDSERPDVIVIDPESHFIGLTESFRKVKRQLPSANIVMVIDDESD
                     FVFDALNSGVCGFIRRGSTKDEILNAVVEAANGGAPLTSKIARMVVESFQKNLETPLT
                     ARETEVMELLSRGKSYSIIADELFIHKETVKSHIKNIYVKLKVKSKAEAIDRAMKDRL
                     I"
     gene            complement(7676..8443)
                     /locus_tag="VGA21_02895"
     CDS             complement(7676..8443)
                     /locus_tag="VGA21_02895"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009195896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HEX9649484.1"
                     /translation="MVKDLSPLPPGKKVYFVSDLHLGSPDYSESLKREKLMVKWLESI
                     SADAHSIFFLGDIFDFWFEYKHVIPKGFTRFLGKVSQLADYGIDIYFFVGNHDMWMFD
                     YFPRELNIPVVKSNMTLKIDGSILLVGHGDGLGQGDTGYKILKKFFSNPFCQWLFARI
                     HPNLGFRIANYWSVKSRKSGEKEISHEKDRLLQYCVNLEKEAHHDYYIFGHRHKPDTY
                     EISESSTYINLGDWIDNFSYGVYASGRVELKTYQAAK"
     gene            complement(8459..10534)
                     /gene="ftsH"
                     /locus_tag="VGA21_02900"
     CDS             complement(8459..10534)
                     /gene="ftsH"
                     /locus_tag="VGA21_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015266230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEX9649485.1"
                     /translation="MSDKKKMMSKGPQKPNYQVWIIVILLAVILGVTYLNKSTSMVTI
                     DPYRFEEMLLSNDVQKVVLIKNQQYVEITLKPGALQNAKYQTELDSSNPFGVSSGPHY
                     RYEIPSVEVFNENFKDVMNKLPRDQQITYTTEEKSDFTQFISTWGFLLLLLFGFWFLM
                     RRMTGGGGPGGQIFNIGKSKAALFDAENKVKITFDNVAGLDEAKEEVKEIVDFLKTPA
                     KFTRLGGKIPKGALLVGPPGTGKTLLAKAVAGEAAVPFFTLSGSDFVEMFVGVGAARV
                     RDLFKQAKEKAPCIVFIDEIDAIGRTRGRGQVPGSNDERENTLNSLLVEMDGFSTDSG
                     VIILAATNRPDVLDSALLRPGRFDRQISIDKPDIAGREAIFKVHLKPLKLSKDVDPKK
                     LAAQTPGFAGAEIANVCNEAALIAARKDRKAVEMEDFHDAIDRVIGGLEKKSKIISPE
                     EKKIVAFHEAGHAVAGWFLEHADPLVKVSIVPRGIAALGYAQYLPKEQFLYQTEQLFD
                     EMCMTLGGRAAEEIVFNKISTGALSDLERVTKMAYSMVTIYGMNDKIGNVSYYDSKQS
                     DYTFTKPYSESTAQIIDQEVKKIISDAFARTKELLKDKREQLNIVATELLEKEIIFQT
                     DLERLIGKRPFNTSTTYQAYTQKSDSLNGTPAENVKPEIPVKDDDKEKPEHLSSDESE
                     EINKESSEN"
     gene            complement(10590..10961)
                     /gene="rsfS"
                     /locus_tag="VGA21_02905"
     CDS             complement(10590..10961)
                     /gene="rsfS"
                     /locus_tag="VGA21_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012927541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEX9649486.1"
                     /translation="MRRKNNPLPSDKLSEIVVRGMQEKKAADILVMDLRNVPNSVADY
                     FVICSGNSDTQIEAISDSVEGVTLKEGFERPYHIEGKKNLEWVLLDYINVVVHIFKKD
                     KRTFYGLENLWGDAVALEIVA"
     gene            11028..11771
                     /locus_tag="VGA21_02910"
     CDS             11028..11771
                     /locus_tag="VGA21_02910"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HEX9649487.1"
                     /translation="MGKKVIYVPTCHSTNLLAEQVLADKGNYEGTVVITDHQTMGRGQ
                     FDREWETEPGKNLTLSVILKPSFLHLESQFLLNMFSSLAVKKTVSFFAGYSEIKWPND
                     ILINNRKISGILIKNVLKGKTITNSILGIGLNVNQEKFTNENAASLRGMTRKQHDLSV
                     VFSSLMENLESYYLKLRESKCKALENEYHNSLFGLEKEIHFESEGEFKGIIKGVDKKG
                     QLIVEVSGKNQKFNLNQIKIHSKGWLGEK"
     gene            11818..13131
                     /gene="ahcY"
                     /locus_tag="VGA21_02915"
     CDS             11818..13131
                     /gene="ahcY"
                     /locus_tag="VGA21_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011584013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HEX9649488.1"
                     /translation="MIETQRKYKVKDVSLADWGRREIKLAESEMPGLMAIREEFGSKK
                     PLKGARIAGCLHMTIQTAVLIETLIELGAEVTWSSCNIFSTQDHAAAAIAAAGIPVFA
                     WKGMSEEEFNWAIEQTLFFGEGEDRKPLNMILDDGGDLTNMVLDNYPELVSGIRGISE
                     ETTTGVHRLQEREKNGSLPLPTINVNDSVTKSKFDNKYGCKESLVDAIRRATDIMMAG
                     KVAVVAGYGDVGKGSAASLRGAGARVIVTEIDPICALQAAMDGYEVKRMETAVAEANI
                     VVTATGNKDIVTSQIFKLMSDKTIVCNIGHFDNEIDVAWLNKNFGSTKEEIKPQVDKY
                     TIDGKDIILLAEGRLVNLGCATGHPSFVMSNSFTNQVMAQMELWNNINKYENKVYTLP
                     KHLDEKVAKLHLAKIGVELTTLTPEQAKYIGVEVNGPFKQEYYRY"
     gene            13423..14010
                     /locus_tag="VGA21_02920"
     CDS             13423..14010
                     /locus_tag="VGA21_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015264145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX9649489.1"
                     /translation="MGESIQQLNKAGKDKLFEMELLPHLSSLYNFAYSLTSNQDDSDD
                     LVQETFLKAYKFLNSYQSGTNAKAWLFRILKNSFINDYRKSSKEPPKVDYQEVESYYN
                     SDDVDIKMTTDLRVDAVNGMMGDEVAMALNNLDVDYRTIIILCDLEGFKYEEMAKILD
                     IPIGTVRSRLHRARQLLKEQLLEYSQKMGFTKRKN"
     gene            14013..14306
                     /locus_tag="VGA21_02925"
     CDS             14013..14306
                     /locus_tag="VGA21_02925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HEX9649490.1"
                     /translation="MTHEISRWIKKNLVTRYIKTECPERRKCLQALEEILDNEAPEDM
                     ESEYKAHIDQCWSCFQDYKLERAIRQLIKIKLERKPVPDKLLEVIKTRISESE"
     gene            14293..14880
                     /gene="gmk"
                     /locus_tag="VGA21_02930"
     CDS             14293..14880
                     /gene="gmk"
                     /locus_tag="VGA21_02930"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009055377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEX9649491.1"
                     /translation="MNQNRSGFKGKAIIISAPSGSGKTSLARNLLKSRNDLDFSVSVC
                     TRPPRLNEAEGVDYYFISNEEFKEKIAKGEFIEWEEVYNGMFYGTLKSEIQRIWTNKK
                     HVLFDVDVKGALKLKKHFGADALAIFIKAPSIEEIERRLTKRMTENSTSMKIRFDKIK
                     LELTFEKEFDVTVLNKEIEEANRNILSIVEKYLAS"
     gene            14877..15449
                     /gene="nadD"
                     /locus_tag="VGA21_02935"
     CDS             14877..15449
                     /gene="nadD"
                     /locus_tag="VGA21_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013926486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HEX9649492.1"
                     /translation="MKVGLFFGSFNPIHTGHLIVAQAVLEQPGINQIWFIVSPRNPLK
                     KNANLLHEFDRLDLVNAAIWDNPKMKAKDIEFRLPKPNYTINTIIALKEKHPKHEFFL
                     IIGQDNLSQFPRWKNHKEILKEVHLYVYPRPNAQNSELDKHSKVKFIEAPLLDISATL
                     IRKSVKKGRSIKYLVPETIEEMIRRKKFYL"
     gene            complement(15451..16011)
                     /gene="frr"
                     /locus_tag="VGA21_02940"
     CDS             complement(15451..16011)
                     /gene="frr"
                     /locus_tag="VGA21_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013455134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEX9649493.1"
                     /translation="MEEVIMLLDEAKSLMEKALQHVNGELAKIRAGKATTSMLDSIRV
                     QYYGNATPLNQVASLSTPDARSIFIKPWEKSLVSEIERSIINSDLGLNPHNDGEQIII
                     NIPILTEERRVQLVKQAKHEGEQGKISIRNARHEVLHSIKALKDEGIAEDEIRKGEEK
                     IDELTSTYTKKIDDLIANKETDILHV"
     gene            complement(16015..16719)
                     /gene="pyrH"
                     /locus_tag="VGA21_02945"
     CDS             complement(16015..16719)
                     /gene="pyrH"
                     /locus_tag="VGA21_02945"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013455135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEX9649494.1"
                     /translation="MKYKRILLKLSGEALMGTRSFGIDPKRLLQYAEEIKKVSEMGVE
                     IAIVIGGGNIFRGSDAERMGIDRVQGDYMGMLATVINGMALQSALEEQGIYTRLMSGI
                     RMDQVCEPFIKRRAVRHLEKGRIVIFGAGTGNPYFTTDSTASLRAIEIEANVVLKGTR
                     VDGVYTADPEKFPDAERYTSLTFQDAYTKGLNIMDMTAFTLCMENNLPIIVFDMNKPG
                     NLLDVIIGKEVGTLIN"
     gene            complement(16792..17295)
                     /locus_tag="VGA21_02950"
     CDS             complement(16792..17295)
                     /locus_tag="VGA21_02950"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEX9649495.1"
                     /translation="MHTIVFESQDKVEFNVEKFDGKWLLNGSEVTINILPINPYYYNI
                     LIDCRSFNLEVLTLNGNSNTPVLRVNNSIISGELRTTLDELLNKMGMYKTIAASVGEI
                     KAPMPGLILQILVKSGMSIKKGEPILILEAMKMENIIKSPVDGIIKNIKAKEKESVAK
                     NQILIEF"
     gene            17478..17562
                     /locus_tag="VGA21_02955"
     tRNA            17478..17562
                     /locus_tag="VGA21_02955"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17512..17514,aa:Tyr,seq:gta)
     gene            17625..17697
                     /locus_tag="VGA21_02960"
     tRNA            17625..17697
                     /locus_tag="VGA21_02960"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17658..17660,aa:Gly,seq:tcc)
     gene            17716..17787
                     /locus_tag="VGA21_02965"
     tRNA            17716..17787
                     /locus_tag="VGA21_02965"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17748..17750,aa:Thr,seq:ggt)
     gene            17877..19064
                     /gene="tuf"
                     /locus_tag="VGA21_02970"
     CDS             17877..19064
                     /gene="tuf"
                     /locus_tag="VGA21_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010611224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEX9649496.1"
                     /translation="MAKETYNRSKPHVNIGTIGHVDHGKTTLTAAITKVLSAKGLAQI
                     RDFSSIDNAPEEKERGITINTAHVEYQTDKRHYAHVDCPGHADYVKNMVTGAAQMDGA
                     ILVVAATDGPMPQTREHILLARQVGVPALVVFMNKVDLVDDEELLDLVEMEVRELLTF
                     YEFPGDSIPVIRGSALGGLNGEAKWVEKIQELMNAVDEHIPIPERLIDKDFLMPIEDV
                     FSITGRGTVATGRIERGVINSGDGVDILGMGAENLKSTVTGVEMFRKILDRGEAGDNV
                     GLLLRGIEKDEIRRGMVICKPGSVTPHSLFKAEIYVLSKEEGGRHTPFFNKYRPQFYL
                     RTTDVTGEIKLPDGVEMVMPGDNVTIEVNLINKVAMEKGLRFAIREGGRTVGAGQVTE
                     ILD"
     gene            19191..19263
                     /locus_tag="VGA21_02975"
     tRNA            19191..19263
                     /locus_tag="VGA21_02975"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19224..19226,aa:Trp,seq:cca)
     gene            19283..19474
                     /gene="secE"
                     /locus_tag="VGA21_02980"
     CDS             19283..19474
                     /gene="secE"
                     /locus_tag="VGA21_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009194212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEX9649497.1"
                     /translation="MQKLIEFFKESYDEMRFKVSWPKYRELQSSSVLVLVASLIFALI
                     IGVMDLGFENILDWFYREF"
     gene            19487..20044
                     /gene="nusG"
                     /locus_tag="VGA21_02985"
     CDS             19487..20044
                     /gene="nusG"
                     /locus_tag="VGA21_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010611223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEX9649498.1"
                     /translation="MTEVKWYVIRVLSGQEKKVKAYLETEIERENLKEYIPEVMIPSE
                     KVYEMRNGKKRVRERNFFPGYVLVQANLEYGEAKHVVISIPGVIGFLGANSTSTSKEP
                     VPLRQSEINRILGKVDEVDEMVEKLDRPYIVGEGVKVMDGPFSGFTGTVEEVFEERKK
                     LNVMVKIFGRNTPVELNYMQVEKLD"
     gene            20045..20497
                     /gene="rplK"
                     /locus_tag="VGA21_02990"
     CDS             20045..20497
                     /gene="rplK"
                     /locus_tag="VGA21_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017732377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEX9649499.1"
                     /translation="MAKEIEGFLKLQVMGGSANPSPPVGPALGSKGLNIMDFCKQFNA
                     RTQDKQGTLIPVLITIYKDKSFDFVVKTPPAPVLILKAANLKKGSAEPNRNKVGSLTW
                     NQCKEIAETKMQDLNAFTIHSAMKMIAGTARSMGITVTGTPPREANVN"
     gene            20499..21197
                     /gene="rplA"
                     /locus_tag="VGA21_02995"
     CDS             20499..21197
                     /gene="rplA"
                     /locus_tag="VGA21_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015264127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEX9649500.1"
                     /translation="MTKLTKNQKNVLENFDPMKSYSLEAASKLVKEITTTKFDSSVDL
                     DIRLGVDPRKADQMVRGVVALPHGTGKQVKVLALVTPDKENDAKEAGADYAGLDDIIK
                     KIEGGWIDFDIIVTMPTVMAKVGKLGKILGPRGLMPNPKAGTVTLDVAKAIREVKAGK
                     IDFKVDKFGIIHASIGKVSFTEDKILDNARELIHTISKLKPASSKGTYFRSISLSSTM
                     SAGISIDKSSIVGI"
     gene            21204..21737
                     /gene="rplJ"
                     /locus_tag="VGA21_03000"
     CDS             21204..21737
                     /gene="rplJ"
                     /locus_tag="VGA21_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010856305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEX9649501.1"
                     /translation="MIREEKATIINELSEKLKENNNYYFTDATGMTVAQINDFRRMCH
                     KVDIDYKVYKNTLIIKALDTLKFDYSPLYEKALKGATGILFSKEIANTPAKLIKEFRK
                     KSNLDKPLFKGAYINQDVYIGENQLDTLSNLKSREELIGEIIAILRSPAQNVISALQS
                     GKNKLAGIVKTLSEKTN"
     gene            21774..22157
                     /gene="rplL"
                     /locus_tag="VGA21_03005"
     CDS             21774..22157
                     /gene="rplL"
                     /locus_tag="VGA21_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEX9649502.1"
                     /translation="MADLKAFADQLVNLTVKEVNELATILKEEYGIEPAAAAAPVMVA
                     GGGAASAEGGAEEKSSFDVILKSPGGAKLAIVKLVKELTGLGLKEAKDLVDGAPKAVK
                     EGLAKDEAEGLKKQLEEAGAEVELK"
     gene            22288..26151
                     /gene="rpoB"
                     /locus_tag="VGA21_03010"
     CDS             22288..26151
                     /gene="rpoB"
                     /locus_tag="VGA21_03010"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009581864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEX9649503.1"
                     /translation="MATKKSNRISFSNIKSVIDYPDFLDVQLRSFKDFFQIETPAEKR
                     TQEGLYKVFCENFPISDSRENFVLEFIDYLIDPPKYSVDECIDRGLTYSVPLKAKLRL
                     SCNDEDNEDFETVEQEVFLGNIPYMTEKGSFVINGAERVIVSQLHRSPGVFFAQSKHT
                     NGTKLYSARIIPFKGSWIEFATDVNNVMYAYIDRKKKFPVTTLLRAIGYGTDKDILDL
                     FGLSEEVSATKANLKKVVGRKLAARVLKTWTEDFVDEDTGEVVSIDRNEVLLERDSVL
                     TKEDVELIIESDTKSIILHRDDVNITDYNIIFNTLAKDNSNSEKEAVEQIYRQLRNTE
                     APDEQTARDIIQSLFFSDKRYDLGEVGRYRINRKLGIDIGFEQRVLTKGDIILIVKYL
                     IGLINSKAVVDDIDHLSNRRVRTVGEQLYTQFGIGLARMARTIKERMNVRDNEDFKPV
                     DLINARTLSSVINSFFGTNQLSQFMDQTNPLAEITHKRRMSALGPGGLSRERAGFEVR
                     DVHYTHYGRLCTIETPEGPNIGLISSLCVHAKVNHMGFIETPYRKVEKGLVSTNVIFL
                     TAEEEDTHNIAQANAKIDDKGKFVNKRVKARYEGDFPVVEPKKVSYMDIAPNQIVSVA
                     ASLIPFLEHDDANRALMGSNMQRQAVPLMKPEAPIVGTGLERRVAEDSRALILAEDEG
                     VVEYSDATKITIRYKQTAEQKSIAFEDDLKIYDLVKFRRTNQDTCINLKPIVKKGDSV
                     KSGTPLCEGYATQNGELALGRNLQVAFMPWQGYNFEDAIVISERVVRDDTFTSIHIEE
                     FELEVRDTKRGEEELTSEIPNVSEEAVKNLDENGIIRIGAEVKEGDILIGKITPKGET
                     DPTPEEKLLRAIFGDKAGDVKDASLKAPPSLRGVVIETKLFSRPKKDKDLRSKSKKDV
                     EILKSKYSNDLRTLRAEMIDKLAALLHGKVSEGVNHKFGESLISKGTKFTAKVISNNI
                     FPEKNVFRDESNYNVPEEVNLISDINLDGWTKDSKTNKLIERLVKNYMAARNKIAGEF
                     KRERFTLEVGDELPAGIVQLAKVYIAKKRKLKVGDKMAGRHGNKGVVAKIVREEDMPF
                     LEDGTPMDIVLNPLGVPSRMNLGQIYETVLGWAGMKLDRKYATPIFDGATIEEVSSEL
                     NEANLPEFGRTYLYDGLSGDKFDQQVTVGVIYMLKLGHLVDDKMHSRSIGPYSLITQQ
                     PLGGKAQFGGQRFGEMEVWALEAFGAANVLQEILTIKSDDVIGRAKAYEAIVKGENMT
                     KPNIPESFNVLVHELRGLALEITLE"
     gene            26185..30498
                     /gene="rpoC"
                     /locus_tag="VGA21_03015"
     CDS             26185..30498
                     /gene="rpoC"
                     /locus_tag="VGA21_03015"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015264123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEX9649504.1"
                     /translation="MSFRKNKKINTDFSTVTISLASPESILETSHGEVTQPETINYRT
                     YKPEMGGLFCERIFGPVKDWECHCGKYKRIRYKGIICDRCGVEVTEKKVRRERMGHIE
                     LVVPVAHIWYFRSLPNKIGYLVGLPTKKLDQIIYYERYVVIQAGIKVEDGVSYLDYLT
                     EDEYLDILDKLPRENQLLEDSDPNKFIAKMGAEALEMLLSRLNLDVMSYDLRHQAATD
                     TSQQRKAEALKRLRVVEAFRDANTRIENRPEWMVIKMVPVIPPELRPLVPLDGGRFAT
                     SDLNDLYRRVIIRNNRLKRLIDIKAPEVILRNEKRMLQEAVDSLFDNSRKVNAVRSDG
                     NRALKSLSDMLKGKQGRFRQNLLGKRVDYSGRSVIVVGPELKLHECGLPKDMAAELFK
                     PFIIRKLIERGIVKTVKSAKKIVDRKDPVVWDILENVLKGHPVLLNRAPTLHRLGIQA
                     FQPKLIEGKAIQIHPLVCTAFNADFDGDQMAVHVPLGHEAILEASLLMLSSHNILNPA
                     NGAPITVPSQDMVLGLYYITKGRKGTKQSPVKGEGLSFYDPTEVIIALNEGRLNKHAN
                     IKVRVKIRNEKEELEIKVIETVAGRVIFNQFIPDEVGYVNELLTKKKLQKIISDVHKI
                     AGTAKAAKFLDDIKDLGFQSAYKGGLSMGLNDVKIPESKTKLIEQAKKEVDVVWNNYM
                     MGLITDNERYNQVIDIWTRINSQITNALMTQLEEDDQGFNSIYMMMHSGARGSREQIR
                     QLGGMRGLMAKPQKNLQGSVGEIIENPILSNFKEGLDVIEYFISTHGARKGLADTALK
                     TADAGYLTRRLVDVAQDLVINEADCGTLRGLVVTALKDNEDIVEPLSERILGRVSVHD
                     IYDPLTEELIVKFGEEITEEIAKKIDETSIEEVEIRSLLTCETRKGSCAKCYGRNLAT
                     GRMVERGEAVGVIAAQSIGEPGTQLTLRTFHVGGTASNIAVEARIRAKFSGTVEFEDI
                     RSVTTKNSEGEKVEVVMGRSGEIRIVDKKSKRVLMTNHVPYGAFLVVKSGVKVEKGDE
                     LCFWDPYNAVILSEVEGTIDFDAIEEGITFREESDEQTGHREKVIIDTKDKTKNPSIV
                     IKKGKEVKSYNIPVGAHLVVDIKDKVIPGQVLAKIPRIMGKSRDITGGLPRVTELFEA
                     RNPSNPAVVSEIDGVVTYGAIKRGNREIFIESKDGVKKRYLVSLSKHILVQDNDYIRA
                     GDPLSDGAITPADILAIKGPTAVQEYLVNEIQEVYRLQGVKINDKHIEAIVSQMMQKV
                     EIIEAGDTNFLQGQTVDKFAFQEENDSVLDVKVVTDPGDSESLKAGQIVSSRKLRDEN
                     SSLRRRDLKIVAVRDAEAAVSRPTLQGITQASLGTESFISAASFQETTKVLSEASIRG
                     KIDLLNGLKENVIVGHLIPAGTGQKRFKNLIVGSQEEYDTLIASKEEFNREKAEEVTQ
                     D"
     gene            30498..30824
                     /locus_tag="VGA21_03020"
     CDS             30498..30824
                     /locus_tag="VGA21_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018476837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HEX9649505.1"
                     /translation="MADSKENKAKQNQINIELSEEIAEGIYANLAMIAHSNSEFVIDF
                     IRLMPGVPKAKVKSRIVITPEHAKRLLHALGENISKYEDTFGPIKKTDETPKFPINFG
                     GTVGEA"
     gene            30993..31367
                     /gene="rpsL"
                     /locus_tag="VGA21_03025"
     CDS             30993..31367
                     /gene="rpsL"
                     /locus_tag="VGA21_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006795815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEX9649506.1"
                     /translation="MPTIQQLVRKGRKKLTSKSKSPALDSCPQKRGVCTRVYTTTPKK
                     PNSAMRKVARVRLTNQKEVNAYIPGEGHNLQEHSIVLIRGGRVKDLPGVRYHIIRGAL
                     DTAGVSGRLQRRSKYGAKRPKK"
     gene            31379..31846
                     /gene="rpsG"
                     /locus_tag="VGA21_03030"
     CDS             31379..31846
                     /gene="rpsG"
                     /locus_tag="VGA21_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013408685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEX9649507.1"
                     /translation="MRKAKPKKRYILPDPKYHDTLVSKFVNCLMIEGKKNKAYNIFYG
                     AIDLVEDKLKENGLEAWKKALNNITPSVEVKSRRVGGATFQVPLEVRPERKISLGIKW
                     LIKYSEARGEKTMTEKLAAEIIAATKGEGGAVKKKDDTHRMAEANKAFSHFRL"
     gene            31850..33982
                     /gene="fusA"
                     /locus_tag="VGA21_03035"
     CDS             31850..33982
                     /gene="fusA"
                     /locus_tag="VGA21_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014798822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEX9649508.1"
                     /translation="MAKKELRRLRNIGIMAHIDAGKTTTTERILYYTGLSHKIGEVHD
                     GAAIMDWMEQEQERGITITSAATTTSWKYPTDQGHTVDKTEEYMINIIDTPGHVDFTV
                     EVERSLRVLDGAVALFCAVSGVEPQSETVWRQADKYKVPRICFVNKMDRSGADFFNVV
                     SEIKSKLGANPIPLQVPIGAEETFKGVVDLITDKAIVWNDDDLGMTYKVIDLPEDLKE
                     TVFEWKQKLVENVAEYDDVILEKFFDNPRSITEKELRAAIRKAVIDMKIYPVFCGSAF
                     KNKGVQAVLDAVCSYLPSPIDLPPVIGANPYSGKEEERKPDANGPFAALAFKIATDPF
                     VGRLCFFRVYSGTLNAGSYVLNMRTEKKERISRLMQMHSNKQNPIDVVEAGDICAGVG
                     FKDIKTGDTLVDEKHPVVLESMSFPEPVIGYAIEARKQADVDKLSIAISKLVEEDPTL
                     TVMTDHETGQTVLKGMGELHLEIILDRLKREFKVEVNQGAPQVAYKEALTKRIEHKEV
                     YKKQTGGRGKFADISFEIGPREDNIPGLKFENAIVGGAIPKEFIPAVQKGFEQAMVNG
                     PLAGYPIDSMHVRLFHGSYHDVDSDSLSFEMAARIGFKDSSKKASPIILEPIMAVEVV
                     TPDEYTGPVTGDLNKRRGIMKGMDSKGGAKVIKADVPLSELFGYVTDLRTISSGRASA
                     SLTFSHYNPVPKNLSESIIEKVKGAPVN"
     gene            33991..34296
                     /gene="rpsJ"
                     /locus_tag="VGA21_03040"
     CDS             33991..34296
                     /gene="rpsJ"
                     /locus_tag="VGA21_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005782187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEX9649509.1"
                     /translation="MNQKIRIKLKSYDHNLVDKSSEKIVRAVKTTGAIVSGPIPLPTE
                     KEKFTVLRSPHVNKKSREQFQLCTYKRLVDIYSNSAKTVDALMKLELPSGVDVEIKV"
     gene            34474..35106
                     /gene="rplC"
                     /locus_tag="VGA21_03045"
     CDS             34474..35106
                     /gene="rplC"
                     /locus_tag="VGA21_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015264117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEX9649510.1"
                     /translation="MSGIIGKKIGMTSIFDAEGRNVPCTLIQAGPCIVTQIRNKETDG
                     YNAVQLAYQDVKDKHTTKALKGHFAKANTTPKRKVVEFRDFRIEFETEVKVGLEVKVG
                     EVFKEGEFLDAIGTSKGKGFQGVVKRYNFGGVGEATHGQHNRLRAPGSIGGSSFPSRV
                     FKGMRMAGRMGGNRVKAINLKVMKIIPEQNLILISGSVPGAINSTIILEK"
     gene            35111..35737
                     /gene="rplD"
                     /locus_tag="VGA21_03050"
     CDS             35111..35737
                     /gene="rplD"
                     /locus_tag="VGA21_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013455152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEX9649511.1"
                     /translation="MNLSVYTHSGSDTGRKISLVKEIFGAEPNDHAIYLDVKQFLANQ
                     RQGTHKSKQRNEVSGSTRKLRKQKGSGAARVGSIKSPLFRGGGRVFGPQPRDYSFKLN
                     KKLKKLARISALSYKAKDKAITVVEDFTFDEVKTRNYVNMLNSLSLGDKKTLLVMPKS
                     DKNVILAGRNIKKAAITTADNLNTYDVLNADNLLVIESSLDRIKELLK"
     gene            35737..36024
                     /gene="rplW"
                     /locus_tag="VGA21_03055"
     CDS             35737..36024
                     /gene="rplW"
                     /locus_tag="VGA21_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014020623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEX9649512.1"
                     /translation="MSVLIKPLVTEKMSALNEKGQYGFVVNKKANKIQIRQEIEKTYG
                     VKVQSVRTMRYGGKEKSRYTKATVIVGKTPSIKKAIVQVADGEVIDFYGGI"
     gene            36037..36858
                     /gene="rplB"
                     /locus_tag="VGA21_03060"
     CDS             36037..36858
                     /gene="rplB"
                     /locus_tag="VGA21_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009578711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEX9649513.1"
                     /translation="MAVKKLKPTTPGQRFRVASTSKELTNNNPEKSLTRSSRKTGGRN
                     NQGRMTMRYIGGGHKKRFREIDFKRNKVGIPGMVKTIEYDPNRSARIALIFYPDGEKR
                     YILAPAGLKIGQQIISGPEVAPELGNALPLLKIPLGTIVHNVELKPGKGGSMARSAGS
                     YAQLLAREDKYATLKLPSGEMRMALVNCYATVGSVSNSDHMNESLGKAGRNRWLGRRP
                     RVRGVAMNPVDHPMGGGEGKASGGHPRSRNGKYAKGLKTRNRNKYSNKLIIGKKK"
     gene            36858..37124
                     /gene="rpsS"
                     /locus_tag="VGA21_03065"
     CDS             36858..37124
                     /gene="rpsS"
                     /locus_tag="VGA21_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010856292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEX9649514.1"
                     /translation="MSRSLKKGPYIDFRLQKKVDSMNESGKKSVIKTWSRRSMISPDF
                     VGHTFAVHNGNKFIPVYITENMVGHKLGEFAPTRTYRGHAKKTA"
     gene            37124..37648
                     /gene="rplV"
                     /locus_tag="VGA21_03070"
     CDS             37124..37648
                     /gene="rplV"
                     /locus_tag="VGA21_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019946169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEX9649515.1"
                     /translation="MEAVAKLNNVPTSPRKMRLVADLIRGQKVSNALNILKFERKNGA
                     PRLEKLLLSAISNWQNKFEDVKLEEAELFVKEIRVDGGRMLKRLRPAPQGRAHRIRKR
                     SNHVTLVIDSLNKLEVKEKVEKTQTEEKQKPVKKEKKKAKTVKAKTGKATTKTKKIVK
                     KSTKKTEKKTKGKK"
     gene            37648..38379
                     /gene="rpsC"
                     /locus_tag="VGA21_03075"
     CDS             37648..38379
                     /gene="rpsC"
                     /locus_tag="VGA21_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009578714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEX9649516.1"
                     /translation="MGQKVNPVGLRLGIIKGWDSNWYGGGTFSDKLVEDQKIRKYVNA
                     RIPKGGIAKIVIERTLKRITLTIHTARPGVVIGKGGSEVDKIKEELKKITRKDVQINI
                     FEIKRPELDAKLVGESIAQQLEARISYRRAMKQAIASSMRVGAQGIKIKVSGRLGGAE
                     MARTEQYKEGRIPLHTLRADIDYAISEAQTVYGKIGIKVWVFKGEVYGKRDLSPNVGV
                     SSSTSGGAQSAGSRRKESGGRRKRR"
     gene            38396..38821
                     /gene="rplP"
                     /locus_tag="VGA21_03080"
     CDS             38396..38821
                     /gene="rplP"
                     /locus_tag="VGA21_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019946167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEX9649517.1"
                     /translation="MLQPKRTKFRRMQKGRVRGIAQRGHTIAFGSFALKSMEPAWITS
                     RQLEAARVAVTRAMKRQGQVWIRIFPDKPITKKPAEVRMGKGKGAPEYWVAVVKPGTI
                     LFESAGVPIALAKESMALAAQKLPIRTKFVVRKDYTESK"
     gene            38821..39015
                     /gene="rpmC"
                     /locus_tag="VGA21_03085"
     CDS             38821..39015
                     /gene="rpmC"
                     /locus_tag="VGA21_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017732358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEX9649518.1"
                     /translation="MKPAEIKKLSSEELREKIKGEKESLQKMKFSHAISPIENPMKIR
                     EAKKLIARLKTELTAKGDAN"
     gene            39017..39277
                     /gene="rpsQ"
                     /locus_tag="VGA21_03090"
     CDS             39017..39277
                     /gene="rpsQ"
                     /locus_tag="VGA21_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015264108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEX9649519.1"
                     /translation="MQATRNLRKERIGEVISNKMDKSITVAVRRKVKHPIYGKFIRKT
                     TKFMAHDEKNECGIGDTVKIMETRPLSKSKRWRLIEIIEKAK"
     gene            39280..39648
                     /gene="rplN"
                     /locus_tag="VGA21_03095"
     CDS             39280..39648
                     /gene="rplN"
                     /locus_tag="VGA21_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003247583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEX9649520.1"
                     /translation="MIQQETKLIVADNSGAKEVLCIRVLGGTRKRYASLGDKIVVTVK
                     SALTSSALKKGTVSKAVVVRTKKEVRRKDGSYIRFEDNAAVLLNPQDEPRGTRIFGPV
                     ARELREKQFMKIVSLAPEVI"
     gene            39651..39998
                     /gene="rplX"
                     /locus_tag="VGA21_03100"
     CDS             39651..39998
                     /gene="rplX"
                     /locus_tag="VGA21_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009183370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEX9649521.1"
                     /translation="MERRYNKQPKLHIIKGDTVMIISGNDKGKKGKVLEVLVSKNRAI
                     VEGMNIVSKHVKPSAKNPDGGIRKTEAGIHLSNLMVIEPSSGEPTRTGRKKNDKGKLQ
                     RYSKKTGEFITNG"
     gene            39991..40545
                     /gene="rplE"
                     /locus_tag="VGA21_03105"
     CDS             39991..40545
                     /gene="rplE"
                     /locus_tag="VGA21_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010611204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEX9649522.1"
                     /translation="MANPRLKELYKKEIVPSLKEKFQYKSLMEVPKIQKISINKGIGE
                     AVSDKKLVDVGVEELTAISGQRAVATLSKKAISNFKLREEMPIGARVTLRGDKMYEFL
                     DRLMNVALPRVRDFRGVNDKGFDGRGNYTLGVKEQIIFPEISIDKVNKINGMNITIVT
                     TAKTDKESYELLKAFGMPFTNRNN"
     gene            40549..40818
                     /gene="rpsN"
                     /locus_tag="VGA21_03110"
     CDS             40549..40818
                     /gene="rpsN"
                     /locus_tag="VGA21_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009284809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEX9649523.1"
                     /translation="MAKESVKARERKRRVLVIRYAAKRKALKAAGDYQGLDKLPRNAS
                     PVRLKNRCKLTGRPKGYMRKFGISRVTFREMASTGMIPGITKASW"
     gene            40911..41309
                     /gene="rpsH"
                     /locus_tag="VGA21_03115"
     CDS             40911..41309
                     /gene="rpsH"
                     /locus_tag="VGA21_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010611202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEX9649524.1"
                     /translation="MTDPIADFLTRIRNAVKANKRIVDIPASNIKKEITKVLFEKGYI
                     KNYKFEDGVNYQGNIKIALKYNPVTKTSALVQLKRVSKPGLRKYSKGADAPRVLNGLG
                     IAILSTSQGVMTDKEARQKNIGGEILCHVY"
     gene            41322..41873
                     /gene="rplF"
                     /locus_tag="VGA21_03120"
     CDS             41322..41873
                     /gene="rplF"
                     /locus_tag="VGA21_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017732351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEX9649525.1"
                     /translation="MSRIGNKIIEIPKDVTIEVKEGNISVKGPKGSLSLEMDSDFKLN
                     QEEGSATLKRPTEQKRHKALHGLYRSLINNMVVGVTNGYKKELEVIGVGYKAAVQGKV
                     LELSLGYSHNIYFGIPEDLKISAETVKGKNPTITIEGIDKQLVGQVAAKIKSLRKVEP
                     YKGKGIRFVGEYVRRKAGKTAAK"
     gene            41881..42231
                     /gene="rplR"
                     /locus_tag="VGA21_03125"
     CDS             41881..42231
                     /gene="rplR"
                     /locus_tag="VGA21_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010611200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEX9649526.1"
                     /translation="MAVTKLVRRRKIRYGIRNKVSGTAERPRLSVFKSNRAIYGQLID
                     DAKAETIVAFNSSKLDLKSNNTVASSKDVGKKLAELAIDKGIKKVVFDRGGYKYHGQI
                     KALAEGAREGGLEF"
     gene            42243..42761
                     /gene="rpsE"
                     /locus_tag="VGA21_03130"
     CDS             42243..42761
                     /gene="rpsE"
                     /locus_tag="VGA21_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016196837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEX9649527.1"
                     /translation="MLQQNIRTVKASEIDLKERVVAIKRVAKVVKGGRRFSFSAIVVV
                     GDGNGVVGYGLGKANEVTDAITKGIDDAKKNLVKVPIHKHTVPHEAIGKFGGGRVLLR
                     PAAPGTGVIAGGAMRAVLESAGVRDVLGKSKGSSNPHNVVKATFDALTRMRDAYTVAQ
                     HRGVQLSKVFNG"
     gene            42768..42947
                     /gene="rpmD"
                     /locus_tag="VGA21_03135"
     CDS             42768..42947
                     /gene="rpmD"
                     /locus_tag="VGA21_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019596883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEX9649528.1"
                     /translation="MAKVRISQVRSTIKRPERQKRTIKALGLGKINRMVEVELTPQIQ
                     GMINKVSHLVSVTEL"
     gene            42949..43398
                     /gene="rplO"
                     /locus_tag="VGA21_03140"
     CDS             42949..43398
                     /gene="rplO"
                     /locus_tag="VGA21_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018476858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEX9649529.1"
                     /translation="MKLSTLKPAKGSVRKSKRVGRGQGSGRGGTSTRGHKGAQSRSGY
                     SSKAGFEGGQMPLQRRVPKFGFKNNNKIEFKAINLDTLENLASQTKVASIDTGLLIKN
                     GLAAKKDKVKILGRGELKTKIEVKAHAFSNSAIEAIEKAGGKAITIE"
     gene            43395..44717
                     /gene="secY"
                     /locus_tag="VGA21_03145"
     CDS             43395..44717
                     /gene="secY"
                     /locus_tag="VGA21_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017732346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEX9649530.1"
                     /translation="MKNFFTTIKNIFSIEELRTRIINTIGYLLIFRLGSYIVLPGIDP
                     VILKSITGDPTGIFGLLNTFLGGAFQRASIFALGIMPYISASIVVQLLTVAVPYFQKL
                     QKEGESGRKRINQITRVLTIAITFMQAFSYLSVTIPAEAITISPFLFKASSIIILVTG
                     TMFAMWLGERITDRGIGNGISMLIMIGIISRLPSQLVAEGLSKGMPGALMFILELVTL
                     FFVVMAVVMFTQAIRRIPVQYAKQVVGNKVYGGQRQYIPLKVNSAGVMPIIFAQALMF
                     IPPLIAGLWADNDISIYIGTVFNDTTSWQYTLVFGILILVFTFFYTAITVNPNQIADD
                     MKRNGGFIPGVKPGKQTSEFIDNILTRITFPGSVFLAIVAIMPTFAVLAGVTPGFSQF
                     YGGTSLLIMVGVILDTLAQIESYLLMRHYEGMMKSGRIKGRSQNVAVA"
     gene            44724..45503
                     /gene="map"
                     /locus_tag="VGA21_03150"
     CDS             44724..45503
                     /gene="map"
                     /locus_tag="VGA21_03150"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002695073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEX9649531.1"
                     /translation="MIHYKTAEEVELIKESAEILSRAHGEVASMIRPGVKTIDLDHRA
                     ESFIKDQGGKPSFKNYKSFPFSLCISLNENVVHGFPGKYELQEGDIVSIDCGVFYKGF
                     HSDCAYTYPVGPVSPDKEELLKITEESLYEGIRNAKAGNRIGDISFSIQEYTEKRGFS
                     VVRELVGHGIGKNLHESPEVPNYGKRGNGPKIKPGLVIAIEPMINMGRRNVVQENDGW
                     TIRTQDREPSAHFEHTVAIFEGKTEILTTHKYIEENFVMNG"
     gene            45496..45714
                     /gene="infA"
                     /locus_tag="VGA21_03155"
     CDS             45496..45714
                     /gene="infA"
                     /locus_tag="VGA21_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017185218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEX9649532.1"
                     /translation="MAKQASIEQDGTIIEALSNAMFRVELENGHQVIAHISGKMRMHY
                     IKILPGDKVKLEMSPYDLTKGRIVYRYK"
     gene            45723..45839
                     /gene="ykgO"
                     /locus_tag="VGA21_03160"
     CDS             45723..45839
                     /gene="ykgO"
                     /locus_tag="VGA21_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014790280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HEX9649533.1"
                     /translation="MKVKASVKKRSADCKVIRRKGKLYVINKKNPRFKQRQG"
     gene            45854..46234
                     /gene="rpsM"
                     /locus_tag="VGA21_03165"
     CDS             45854..46234
                     /gene="rpsM"
                     /locus_tag="VGA21_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011586488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEX9649534.1"
                     /translation="MARISGVDIPDSKRGEVGLTYIYGIGRRSAQKILTKAGVDWNKK
                     VSEWNDEESNKIRSIITDNFRVEGSLKSEVQLSLKRLLDIGCYRGLRHRKGLPVRGQK
                     TKNNARTRKGKRKTVANKKRAPKG"
     gene            46249..46641
                     /gene="rpsK"
                     /locus_tag="VGA21_03170"
     CDS             46249..46641
                     /gene="rpsK"
                     /locus_tag="VGA21_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012930219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEX9649535.1"
                     /translation="MAEKRKDKARKRVVHVEAVGQVHIQATFNNIIISITNMQGQVVS
                     WASAGKMGFKGSKKNTPYAAQVAASDCSQKAFDLGLRKAEVFVKGPGSGRESAIRTIQ
                     STGIEITTIKDVTPLPHNGCRPPKRRRV"
     gene            46653..47255
                     /gene="rpsD"
                     /locus_tag="VGA21_03175"
     CDS             46653..47255
                     /gene="rpsD"
                     /locus_tag="VGA21_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019596889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEX9649536.1"
                     /translation="MARYTGPKAKVARRFNDPIFGPSRAVQKKAYPPGQHGRNRRRKQ
                     SEYSLQLMEKQKAKYTYGVLERQFENLFHTAAAKPGITGEVLLQLLEARLDNTVYRLG
                     IAPTRRAARQLVLHKHITVNGKVVNIASFEIRIGDVVAVREKSKSLLAITESLAANPK
                     KFPWLEWNASALEGKLISMPIREDIPENIQEQLIVELYSK"
     gene            47297..48286
                     /locus_tag="VGA21_03180"
     CDS             47297..48286
                     /locus_tag="VGA21_03180"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010611192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEX9649537.1"
                     /translation="MSILAFQMPEKVVMEKSDDFHGLFTFKPLEKGYGVTIGNALRRI
                     LLSSLEGYAITGVKIPGVLHEFSTVEGIVEDVSEVILNLKMVRFKKIAESIDSKIIVS
                     VKKQSVLTAGDLIRFTNSFEILNPDQVICNLDESVNLELELTVEKGRGYLPAEENKPS
                     EQIFGFIPIDAVFTPIKNVKFSVENTRVEQKTDYEMLILDIETDGSIHPENALKGAAN
                     ILIQHFMLFSDQNMVLEPTDSGEPEAVDEEMLHMRKLLKTPLNDLDLSVRAYNCLKAA
                     DVKTLGDLVRLEISDMMKFRNFGKKSLAELEQLIAEKNLTFGMDLSKYKLEED"
     gene            48292..48915
                     /gene="rplQ"
                     /locus_tag="VGA21_03185"
     CDS             48292..48915
                     /gene="rplQ"
                     /locus_tag="VGA21_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020532206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEX9649538.1"
                     /translation="MRHGKKFNHLGRTSSHRKSMLSNMASSLILKKRIKTTVAKAKAL
                     RLYVEPLLTKAKSDTTHSRRVVFSYLQNKYSLQELFGDIASRIAERPGGYTRILKTGN
                     RLGDNAEMCIIELVDYNEGLLKTEKSAKSKTRRGRSKKKYSDSEDTMTTAEVVEETTA
                     AVEVVAEEESVETKKPEASSETQEGTTDDESKQNPEAEGSDEKNKEN"
     gene            48996..50111
                     /gene="carA"
                     /locus_tag="VGA21_03190"
     CDS             48996..50111
                     /gene="carA"
                     /locus_tag="VGA21_03190"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017732338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEX9649539.1"
                     /translation="MKIASRKKAYLVLQDGLILQGISIGKSGTRGGEICFNTGMTGYQ
                     EIYTDPSYFGQIIVSTAAHIGNYGAMSTEQESSGAKISGLVVNEFSQVYSRETADESL
                     QNYLERNEIVGIADIDTRKLVRHIRSKGAMNAIISSELEPDALKTELNKIPSMEGSEL
                     SSVVSTKEPYFVGESKNSFRIAALDLGIKKSILTNLVERGCLVQVFPAKTSFTDMEKW
                     DPDGYFISNGPGDPAAMEYAVETVKEVLNKNIPLFGICLGHQILARACGISTYKMHHG
                     HRGLNHPVKNLNTGRSEITSQNHGFCVNGDETSKNKNLKITHVNLNDKTIEGFEVLNK
                     KAFSVQYHPEASPGPHDSRYLFDNFIEMVKKSKKINV"
     gene            50108..51382
                     /gene="eno"
                     /locus_tag="VGA21_03195"
     CDS             50108..51382
                     /gene="eno"
                     /locus_tag="VGA21_03195"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009285777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEX9649540.1"
                     /translation="MSFIEEIIARQIFDSRGNPTIEVDVLTENGFIGRAAVPSGASTG
                     SHEAVELRDGGPNYMGKGVLKAVKNVNELISPELIGMPVMEQTLIDNLLIQLDGSPNK
                     SKMGANAILGASLAVAKAAAQESGQPLFRYVGGVNACTMPIPMMNILNGGSHADNSID
                     FQEFMIMPMKASSFSEALRMGTEVFHNLKKVLTSKGLSTNVGDEGGFAPNIKSNVEAI
                     ELVLISIEKAGYKPGEDIFIALDAASSEFYDIKKSRYILKKSTREELSSSELAAFWKE
                     WVDKYPIVSIEDGMAEDDWDGWKELTNVIGSKVQIVGDDLFVTNVERLQKGIDEGIAN
                     SILIKVNQIGTLTETINAVNLATRNSYTSVMSHRSGETEDNTIADLAVALNTGQIKTG
                     SASRSDRMAKYNQLLRIEEELGEVAYFPNKLK"
     gene            51455..51748
                     /locus_tag="VGA21_03200"
     CDS             51455..51748
                     /locus_tag="VGA21_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017733812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEX9649541.1"
                     /translation="MRVKLPAYLKNFYFIIAVFFLIWMLFVDSNDVFSQLKLRKKVTT
                     LEQEKEYYLEKIEEVKKDREELLSNQELLEKFAREKYLMKKKTEDIYLVVEEK"
     gene            51754..52290
                     /locus_tag="VGA21_03205"
     CDS             51754..52290
                     /locus_tag="VGA21_03205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649542.1"
                     /translation="MKKFLLVPFILVSFPAICQYDQLIGKDVPLIERFYVGGGFGLGF
                     SSFSDYVAVSPNIGYRITERGSIGIGVLYQYRNYKAYDVKTDDWGGSIFTRYKVFGPA
                     FAAAEYEYINFEFVTATLTTFRGDYSSFMVGAGIAQPITRNVSFIISAMYNLTYRETD
                     GGPYSSPWNLRVGISAGF"
     gene            complement(52287..53354)
                     /locus_tag="VGA21_03210"
     CDS             complement(52287..53354)
                     /locus_tag="VGA21_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019597589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HEX9649543.1"
                     /translation="MVIPARLKEFCTNVFLKMGCSPIDANLAADVLISADLRGIDSHG
                     SARLPGYVRLWEAGRINSKPVIRITHETPGTAVVDGDRGLGLVVSPFAMEIAIKKAKK
                     VGTGWVAVRNSNHFGIAAYHSMLALQHDMIGISMTNASPLVAPTFSKERLLGTNPISV
                     AIPAGTEPPYVADFATTTAANGKLEILQRQGGKAPIGWVQTKEGLPSQDPNEVQNGGA
                     LLPLGGIRETGGHKGYILGSLVDIFSAILSGANYGPWVPPFVSFLPVRNDLVGDGIGH
                     FFGVMRIDAFRDAKEFKENMDKWIGRFRNAQTIDGQERVLIPGDVEREEEIKRLKNGI
                     NLDPKVEESLKVLAEKFGLDF"
     gene            complement(53363..54304)
                     /locus_tag="VGA21_03215"
     CDS             complement(53363..54304)
                     /locus_tag="VGA21_03215"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HEX9649544.1"
                     /translation="MKTRVGIVDLGTNTFHVLVAEMEGMEYSFIHKEKIHVALGQGGI
                     NTGVITPESANRAFSAALHIKNLLDLYKVERTKCVATSALRSSGNSSEIIDQLTGITG
                     MDIEIISGEKEAAYIFEGVSLALKMDEADGLIMDIGGGSVEFVIGNSSGIKWKKSLEI
                     GAQRLSDKFQENDPILPGEIRNVEQYLIEKLTPLYQALNVYKPKILIGSSGTFDTLSE
                     IYRRKNDIAIVPDQTELPLSIEGMKDIQLEIISKNRAQRLQISGMIEMRVDLIVVASI
                     LIQSVIMMNSFEGVRVSSYSLKEGIFSEFLKSQKYLV"
     gene            54435..56018
                     /locus_tag="VGA21_03220"
     CDS             54435..56018
                     /locus_tag="VGA21_03220"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009197594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HEX9649545.1"
                     /translation="MAKKTSKSKSKWVTSKEKFALLERKNKQALLGGGEDRIIAQHNK
                     NKLTARERIELLIDNGTFNEIGKFVKHRSKDFGLDKVQVLGDGVITGYGLVNGRSVYV
                     FSQDFTILGGSLSETHAEKIVKLMDLALKNGIPLIGLNDSGGARIQEGVVSLGGYADI
                     FYRNTLASGVIPQISAVMGPCAGGAVYSPAITDFILMVEHTSYMFVTGPNVVKSVTQE
                     EVSAEELGGTSTHSTKSGVTHFSCANEVECIQQIKQLLSYMPQNCEEQPPLFEYDPSE
                     ELRPDLDTIIPDNPNQPYDIREVIDQIIDGNTFLEVHENFAENIVVGFARLAGRSIGI
                     VGNQPSVLAGVLDINSSIKGARFVRFCDSFNIPLLVLVDVPGFLPGTDQEWNGIITNG
                     SKLLYAFSEATVPRITVIVRKAYGGAYDVMNSKHIGADLNYAWPSAEIAVMGAKGAAE
                     IIFRKEIQSSKDPQKKLDEKIDEYTKKFAHPYIAAARGFIDEVIYPRETREKLIGAFK
                     MLENKVDKIPSKKHGNIPL"
     gene            56023..56724
                     /locus_tag="VGA21_03225"
     CDS             56023..56724
                     /locus_tag="VGA21_03225"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9649546.1"
                     /translation="MNEKVIIVTAASKGIGRACAIKLAEQGCKLSLMSRSEEISEVLP
                     GKDFQATIGDVGKLEDLEKLVAHTMSKFGRVDGVVNNTGHTAKGDLISISDQEWQQAF
                     EIAFLNTVRMARLVTPLIQRQGTGSIVNISSFAAKEPSLLFPTSSVIRAALSNYAKLY
                     ATRFGHRKIRMNNVLPGYVDSYPLDKETIKTIPMGRPAKTEEIANLVAFLISDESSYI
                     NGQSVVIDGGLNKSF"
     gene            56727..57806
                     /locus_tag="VGA21_03230"
     CDS             56727..57806
                     /locus_tag="VGA21_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009578745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HEX9649547.1"
                     /translation="MDKKSEKFLYQYLNNASPTGFESSGQQIWLDYIKSYVNKYFTDT
                     YGTAVGVINPDAKYKVVIEAHADEISWFVNYITKEGYIYVIRNGGSDHIIAPSKRVNI
                     HTDKGIVKGVFGWPAIHVRDKDSEKAPALDNIFIDVGCHSKKEVEQLGVHVGCVITFE
                     DELTTLNKRYLVGRALDNRIGGFMIAEVARRLKEKKKKLPFGLYVVNSVQEEIGLRGA
                     QMIAQKISPDLAIITDVCHDTQSPMYNKIKSGDQKSGDGPVLTYGPAVQNNVLKFIIE
                     VAKKNKIPFQRQAVSRSTGTDTDSFAYSNEGVASALISLPLKYMHTTVETVHFADIEN
                     VIKLIFETVLSVKPGQNFKYLNKAT"
     gene            complement(57799..60807)
                     /locus_tag="VGA21_03235"
     CDS             complement(57799..60807)
                     /locus_tag="VGA21_03235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649548.1"
                     /translation="MNCWNSIFSFLLIFDLFCACQYSNAQTKTWINSSLDGDWNNPVN
                     WTPPGTPLSTDVVYFSASSSNYDAKLSASISLAGLYITDFTGTIDLDGNDVSIFGEVE
                     ITSGSIFSSIGAPFLIIFNSGTTRFENTSLEVITYSLSSDVYLSGNEFIESVYFSKSG
                     NADNYSAGDNIFYTNSVLEISGAGNLIMGNLSGDTFEDLIDLNNYGTGTLWLGYSGTS
                     NFSSTISIDNAVGNIHFGNATSQINFNPGANIKISNFTGGTLTLQSIQDFNTDLMDLI
                     LTDGATLNIKSCIFNRPIQIESPRFFTSFSRFRNSLSVEKTGDLDDASLGGNQYDGDL
                     SITNSSNYFILFGANAATPPDVYNGQVTLNNYGSLSRIFMSGYSSGNVFNGPVIVNQE
                     STGGGGYIRFSEFSSSSSTFNNSISVNCTSGAGVYLGNAGSVTLNPGAIVNIGSTGFE
                     TGTLRLRNVSQFGSASTYNLSLTGSSTLNFINTIWNAPVIVETPILLLEGNSFNNSSS
                     FKTTGTINSYCPGANTFNSNVTFENAGTARLRLAYDPGKPDDFNGNLTLIKSNTGFLQ
                     PCYNSDCTIAGDIIINTNSSSPAFGFSELSGTTILDGSTNQNFVRLDGTSNIPSFRNL
                     EINKSGGTASLGFRLQILDFLSLVNGIIETNSFDVTIENGANSSTGNNGSFIEGTVAK
                     RGNIPGDFTFPTGDAGIWAPVSISILTGGTSSNLFAASYHYQEPVDMANLDAAINHVS
                     SLEYWTLNRLSGTATGDVTFFWKDANQSDIDVLSSDLIISKYSGTGPWQNIGGSITPG
                     SSIGAGGEGGITANVTSFSTFTFGKATGITGLPVNEIELSTNKLGSQISLDWKTNPTS
                     IYIAYEIQKSLNGSEFQTFAEVTPVNGKSSYSYMHLDEVPGQAYFRILGISDGNKQKF
                     SNVLFLSNSGKFDFHIIKSESIYSLILENFNGRNLAMKIVDQTGREVYTKQFNDPEIR
                     IELPTLISNLYIVTLTGDGVFRSKKILIIR"
     gene            complement(60875..62107)
                     /locus_tag="VGA21_03240"
     CDS             complement(60875..62107)
                     /locus_tag="VGA21_03240"
                     /inference="COORDINATES: protein motif:HMM:NF012497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEX9649549.1"
                     /translation="MKSESLLLKKSQLKEGAKITLPPSKSESNRYLMINALSGSISTL
                     LNLSSSRDTLIFQRALTNSGKTIDVGDAGTAMRFLTAYFAVTGKRKILKGSERMHKRP
                     IGDLVEAFKKLGANIKYLGSKGFPPIEIADFVHSGKNKVQISGGISSQFISALMMIGP
                     KLDGGLTITLAGKVVSKPYILLTSSLMGQFGIKPIVKASSIFIKEGKYRKRKVKVDGD
                     WSAASYWYSLFRLSNAKRILLTGLSHHPRQGDAKLISIMKGFGVRSRPIPGGYELTRI
                     KFRQRKIFDFIENPDLAQTIIVLCALLKVRAKFNGLDTLSIKETDRIKAIQKELSKIG
                     VAFLKNQDNSYSLSFLTISGELPKQIRIRTYEDHRMAMAFAPLMLFTDVLIENPSVIN
                     KSYPGFWNQLESVMEFAN"
     gene            62220..62399
                     /locus_tag="VGA21_03245"
     CDS             62220..62399
                     /locus_tag="VGA21_03245"
                     /inference="COORDINATES: protein motif:HMM:NF024143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEX9649550.1"
                     /translation="MGSTGKAFALLVGLAAGAALTVFAFSKKGKKTREEIARKFNEIA
                     DGVKTSVRKSLTKVS"
     gene            complement(62396..63442)
                     /gene="aroB"
                     /locus_tag="VGA21_03250"
     CDS             complement(62396..63442)
                     /gene="aroB"
                     /locus_tag="VGA21_03250"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEX9649551.1"
                     /translation="MLPENLMFTSSVSNDLEGFFSKNHFSRTGILVDENTKKHCLPLI
                     SSAVSHCGIIEIKSGEVYKNVETCNHIWTSMTNAGFDRSSLLINLGGGVICDMGGFCA
                     ATFKRGIEFINIPTTLLAQVDASIGGKTGIDFLSLKNQLGVFSEPFAVIIDPVFLSTQ
                     DKRHVKSGFAEIIKHSLIADNGYFQYLLTTSFDEIKWDKVIQRSIEIKHSFVVQDPFE
                     EEVRKSLNFGHTIGHALESYYMEKNNQLLHGEAVAAGLLCESFVSALKLGLPDPALQD
                     ISKLIVHNFGCRKISPESQPEIMNYLKNDKKNIEGRILMTLISGIGKVKVNVEVSVEE
                     IEKSFSFYNHLVKA"
     gene            complement(63445..64509)
                     /locus_tag="VGA21_03255"
     CDS             complement(63445..64509)
                     /locus_tag="VGA21_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004156191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-deoxy-7-phosphoheptulonate
                     synthase/chorismate mutase type II"
                     /protein_id="HEX9649552.1"
                     /translation="MESFFRKEKPFIIAGPCSVESYEQLALVAGALKEMNIPLLRAGS
                     WKPRSRPDTFEGLGVLALEWLGKIRNEFEIKVTTEIANPYHVEQALKYEIDVFWIGAR
                     TSVNPFVVQEIAEALSGIQRPVLIKNPINPDLGLWLGSIERIEKKTKDVAAVHRGFSS
                     FQPSRFRNIPLWQIPLELRRIRPDLPLICDPSHIAGKRSMILEIAQKSMDLNYDGLMV
                     EVHPDPETALSDSEQQITPTSLGELLNRLEIRSENFTDPLFLDLLEELREKINIADRD
                     LIEALSTRMKLVEQIGDYKKENNVAVFQLERWKEILKSRPEWAKVLGLSPRFIEELYK
                     VIHEESIRIQTAINSKKESK"
     gene            64611..65792
                     /locus_tag="VGA21_03260"
     CDS             64611..65792
                     /locus_tag="VGA21_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009578755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HEX9649553.1"
                     /translation="MDLKTYVDFDNTEVAFSSKSNRKLFKARLLFWVVNHPFLAAIST
                     GLVRVVIFFGLPVNWIIKPTVFEHFCGGETIKESEGSIAELASFNIKTILDYSVEGED
                     SEEDFETTKRETLRTIEKAAQNSNMAFCVFKPTGLASQIIMEKVQLRKNLTDEEGRNW
                     QKAAERFDAICNAASEMKIPILIDAEDSWIQDPIDKLAMDLMRKYNRETAIVFNTYQL
                     YKKNALQDLKRDCERARTEKFFLGAKLVRGAYMEKERERAEEKGYPDPINESKEDTDR
                     DYNAALEFCIENIGQLYLFSGSHNEYSNYYLTLLMEKQGLAKNDKRIFFGQLYGMGDH
                     ISFNLAAAGYNVAKYVPFGPVKSVMPYLFRRAEENTSVKGQSSRELTLIRKETLRRKS
                     G"
     gene            65838..67568
                     /gene="lysS"
                     /locus_tag="VGA21_03265"
     CDS             65838..67568
                     /gene="lysS"
                     /locus_tag="VGA21_03265"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009578759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEX9649554.1"
                     /translation="MELSEQELIRRQAKAELEKLGINPYPPEEYPVTTTSGEVTSGFE
                     ANPEAFKDLSIAGRIMSRRIMGSASFFEIQDSSGRIQAYVRRDDICPNDDKTFYNTVF
                     KKLLDIGDIVGIHGFAFKTQTGELTIHVSELKILSKSLRPLPIVKETVEVDGSIKVHD
                     AFKDPEARYRQRYVDLIVNPQVREVFRKRTRLMNSMRRFLDEKGYLEVETPVLQPLYG
                     GAAARPFKTHHNALDSTLYLRIANELYLKRLIVGGYDGVYEFSKDFRNEGMDRFHNPE
                     FTQIELYVAYKDYNWMMELVEAMIEKVALDLHGTTVVTVGDNKIDFRKPWKRYSMFEA
                     IQHFTGVDISKMDELELRKTAKEMGIEVEATMGKGKIIDEIFGEKCEPKLIQPTYITD
                     YPLEMSPLAKKHRSVDGLVERFEAIANGKEICNAFSELNDPIDQRQRFEMQLELGLRG
                     DEEAMMMDEDYIRALEYGMPPTAGLGVGIDRLTMMMTNSHSIQDVLFFPQMKPEKKPG
                     VSNDAELEQLGINAGLIPAIRKLGINTIQQIKSSNPNKLFNDVCGTRKKMKLDVANPT
                     LEEVKKWIES"
     gene            complement(67569..70145)
                     /locus_tag="VGA21_03270"
     CDS             complement(67569..70145)
                     /locus_tag="VGA21_03270"
                     /inference="COORDINATES: protein motif:HMM:NF040057.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative LPS assembly protein LptD"
                     /protein_id="HEX9649555.1"
                     /translation="MYILRTSFSFLLFFLFSFTGFAQIPVQKPILNQADTLQSSLNAP
                     NDSISADSVVRKGDIETTVKYFAVDSIRFSVDAKHVYLYGNARIEYGEIVLTGERIEV
                     DYNDNLLIANPVIDSTGKMIGKPVFQEGESRYETTGIKYNFKSKRAYISGVVTEESEG
                     FMHGDEVKKDELDHLYIRNAKYTTCNLEHPHYYIQARRLIVVPSEKVVAGPFNLNIND
                     VPTPIGFPFGMFPAPDQRASGVIFPTYGEEVRRGFFVRNGGFFWAINDYVNFAVTGDA
                     YTTGSYGINGSSTYKKRYKYTGSLRWNYTSTKLGEEGREAVSKDFRINWSHTPQNYGS
                     SRFSININAATNTFNQNNALQIEDNINRTLNSSISYTKSFTGTPFSMGLNSRFTQNLT
                     TKEVTFLLPDLSVNMQDQFPFRKRNQSPRNVIERIKFRYTLSGRNQLNNRISRDSVSA
                     FNFENMGDLFGAAKRGLSHSIPISTSMSLFKFFTLTPSFNYTERWYFERFDYVDDGQT
                     KVKSDTSSGFYRVYDYSTNASMTTRLYGTLLFKKGYLRAIRHTLIPTVSVSYRPDFGK
                     QKFGYYEQYDDANGNLKYANRFESAIYGVPGTGRNGSIGFSLGNNLEAKVLDKSDSLQ
                     KTKKVTLLNNLSVSSGYNFLAERYRLSNISISTATSLFGGKLNASINGSIDPYVYRED
                     DAGNQVKLDEYTWEHGKGIGQLSSLTIGLSSSLNPATFSTKEKIEKSKAGDAEKEFML
                     NNPNLYVDFDLPWNLSANYSLTRSKIGLADPNITQSLSFNGDLRITQNTMITFTSGYD
                     FESKEFTQTQFSLTRNLHCWEMLFTWTPFGRFESYDFTIRVKSSMLQDLKINRRRSFF
                     DR"
     gene            70221..71000
                     /locus_tag="VGA21_03275"
     CDS             70221..71000
                     /locus_tag="VGA21_03275"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018477076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEX9649556.1"
                     /translation="MKNITVISILSAIILLGSFTPLELRKMKVSRIVVDAGHGGQDPG
                     THGDFSKEKDVALKIALKFGRYVETLLDEVEVIYTRSDDRFLELEQRAQIANKNGADL
                     FISIHCNAIPRTEIHGTETYVMGLDKVEGNLMVAKRENSVILQEDNYEEVYEGFDPNS
                     PEANAIFALYQSAYLESSLKLAYKVETQFKTRVGRYSRGVKQAPFWVLWRTSMPSVLI
                     ETGYLTNPNEEKFLNDELKQDYIASAIYRAFRDYKKELDSM"
     gene            71011..71967
                     /locus_tag="VGA21_03280"
     CDS             71011..71967
                     /locus_tag="VGA21_03280"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEX9649557.1"
                     /translation="MKYSKEIKVGFFSVLTIAMLYLGVNYLKGVDFFSSVSRYYVMYD
                     DVGGLQVSNPVKINGFTVGRVSDISILQEKQNKILVEIEIKEEIILGDTTLALLDVEF
                     LGAVNIILEVGDISNPLSSGDTLRPKLDQGLEDLLRSSALPVADNLQGTIRKINTFLD
                     DLSGNLEHLGPALENISGAARNVKTITAGSNQEKLSDLLDNLNSTAVELKATIKKIDP
                     LVQNFTQVADSLKSLQINETLAKMNTTLEGLDQTLTDIREGDGTISRLIKEDSLYVQL
                     NQTLQNLDKLLIHFDEFPKDFLSPLGRSSKKIQKRIEKEGNN"
     gene            72025..73653
                     /locus_tag="VGA21_03285"
     CDS             72025..73653
                     /locus_tag="VGA21_03285"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009185010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HEX9649558.1"
                     /translation="MNLEFNRNEDKYKMLVSELKRKLAEIKLGGGKSKIDSQHKKNKL
                     TARERIEYLVDPASDFLEIGAFAADGMYKEVGGCPSAGVVTGIGYISGRQCVIVANDA
                     TVKAGAWFPMTAKKNLRAQEIAMENRLPIVYLVDSAGVFLPMQDEIFPDKEHFGRQFR
                     NNARMSSMGIIQIAAIMGSCVAGGAYLPIMSDEAMIVNKTGSIFLAGSYLVKAAIGEE
                     IDNETLGGATTHCEISGVTDNKYETDQDALDAIKDIFSKLGNFDKAGFNRIKSKPPIE
                     NQEEIYGLIPEDGLKPYDTMQIIRRIVDNSEFQEYKGLYGKTIICGTGRIDGWSVGIV
                     ANQRKVVKSKKGEMQMGGVIYSDSADKAARFIMNCNQRKIPLVFMQDVTGFMVGSRAE
                     QGGIIKDGAKMVNAVANSVVPKFTFIIGNSYGAGNYAMCGKAYDPRLIFAWPSAQIAV
                     MSGNSAAKTLLQIKISAFEAKGKKLSKEEQEQHLKEIKDTYNSQLNPYYASARLWVDG
                     IIDPLETRKIISMGIEAANNAPLSNKFNVGVIQT"
     gene            73708..74538
                     /locus_tag="VGA21_03290"
     CDS             73708..74538
                     /locus_tag="VGA21_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015812358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HEX9649559.1"
                     /translation="MNKKEIKALVSLLDDDDKEVLINVEQKLLSLGGDVIPLLESEWE
                     YNFNPTVQRKIEDIIHVLQFENLLDRFHRWKNQKGKDLLTGMWLVATYQYPDLELENL
                     TKELEQIYYEVWTEFKPDLYPYDQVKVLNGVLFSKLRFSANTKNFHSPSNSMINSVLE
                     TRKGNPISLAVVYLLIARKLNMPVYGVNLPNLFILTFKNKETQFYINAFNRGLIFSRE
                     DIDNYISELHLNKQDTFYEPCTNLDIIQRVLRNLMVSFEKIGDHYKVDEVKRLLNSIL
                     "
     gene            74861..75886
                     /locus_tag="VGA21_03295"
     CDS             74861..75886
                     /locus_tag="VGA21_03295"
                     /inference="COORDINATES: protein motif:HMM:NF024829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEX9649560.1"
                     /translation="MKTGKFYLSFLPIILLLSCMNDSDKKMEVISVDVAEARIDSIAK
                     KLVAAGKVVAEKEFLIPSPMRGSVKEIIARNGLWVDSGAVLIKLKDDYFRMDLEKAKA
                     AKSAKEIVFQKMTLDYVDINDWESEKGQDVRKMLRSYSGLEVAEINLQEALEALDNTS
                     IRTPVGGIVDQLVVQEDEYLSDGQLLCKVYHPAHMEVITFFPEEEGIYLYKDQEASVY
                     IDDAIESAPGIIKWVDNKPNENGMIKVLISLEGDNLLPDKPARVETISKYWAGMLIPV
                     DGVLLKNGKPAIYKEVEGKAIFQFVELGMASGQEIEITSGLLLNEKVIISNLNLLESN
                     SQVTINN"
     gene            complement(75892..76476)
                     /locus_tag="VGA21_03300"
     CDS             complement(75892..76476)
                     /locus_tag="VGA21_03300"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEX9649561.1"
                     /translation="MTGRQTIFFLLLLINAQGFPQGKKYVEDFTLKDAREGKNYSLSN
                     FRNAKGILVIFNSTECPYSLHYDSRIGALAKKYSDHGISFLMINSNFGSKESAESLDA
                     IKSHANTLDFPYLADIAQDAMNKFGARKNPEVFLLKPENGKFGIFYRGAIDDNPQVEH
                     DVRTPYLDNAINQLLLNRNANPAFVNPAGCIIKK"
     gene            complement(76473..77132)
                     /locus_tag="VGA21_03305"
     CDS             complement(76473..77132)
                     /locus_tag="VGA21_03305"
                     /inference="COORDINATES: protein motif:HMM:NF013786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HEX9649562.1"
                     /translation="MASQLSRTYMPVIHKEENSEYRWGIWQIEESESELLRDLPSDED
                     LRNSLATIKVFNRKIESIATRALLKYLASDAGVRYNGIWKDKHGKPFLIDEKTHISIS
                     HSYPYAAAMLNLNDPVGIDLEHYNKKILKLETKFMSEQEIFDSNKDPGKMILVWAAKE
                     ALYKSYGRKNLSFKDNIEILPFEMLKEGILKSKVKRHDEVRFYELEYKLFPDFVVCYT
                     L"
     gene            77505..78230
                     /locus_tag="VGA21_03310"
     CDS             77505..78230
                     /locus_tag="VGA21_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009583150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HEX9649563.1"
                     /translation="MKITPLEIRQKTFEKVFRGLDKDEVQAFLNTLSQEWEKVNQECK
                     ELKIRLETSEREVQKLREVESSLFKTLKTAEDTGANLIDQATKTADLHMKETQMKTEG
                     LMNEAKSKAKELMEEAEDYARNTLVKMEDELKNLADSYRDLESQRENLIDELARLTNE
                     SLEKLNRSRAKMKSFDVSSYLKKTKVESFMASIKEDQLGNKTISQEPSQKVQHKAEKS
                     PDSGSPSKGEEQETNSQSFFDNI"
     gene            78230..78592
                     /gene="folB"
                     /locus_tag="VGA21_03315"
     CDS             78230..78592
                     /gene="folB"
                     /locus_tag="VGA21_03315"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HEX9649564.1"
                     /translation="MRSKWVISLRGIKIKAFHGLYEFEKKEGNNFLVDVSVKLTDDQL
                     TISDLDSTVNYENLYKIVVEQMKIPTDLLEEVVKRIGEKILEEYKIVSRVEVSLAKLN
                     PPMMDDCAEAKVTIKLSR"
     gene            complement(78595..78897)
                     /locus_tag="VGA21_03320"
     CDS             complement(78595..78897)
                     /locus_tag="VGA21_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009583148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HEX9649565.1"
                     /translation="MSEKIMPVSFTDRAVAEVKNIISSKKIPVDYGLRVGVRGAGCAG
                     VSFLLGFDRKKDQDDEFELEGIRIYIEKKSTLYLAGQQVDFYNGDEARGFIFTAEK"
     gene            78972..79493
                     /locus_tag="VGA21_03325"
     CDS             78972..79493
                     /locus_tag="VGA21_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009578293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649566.1"
                     /translation="MDTLIEFAKLLVPAGLVLYAMYLVVKSFLEKDFEKKVVDLRTKN
                     QETVLPIRLQAYERMTLFLERITPSNLMPRIKTGTLNAKEFQQVLLNEIRAEFNHNAA
                     QQVYMSQEAWDMIKNAKEDLIVTINNSAAQLSDEASESDLSRKIFETTMETELDAIGN
                     ALRFIKDEIQQYF"
     gene            79500..79922
                     /locus_tag="VGA21_03330"
     CDS             79500..79922
                     /locus_tag="VGA21_03330"
                     /inference="COORDINATES: protein motif:HMM:NF018508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HEX9649567.1"
                     /translation="MEKEGFGNEHFEKAAEKARKIAGNKDRLIDLVTGVTQKITAELK
                     GKFGAARLKEKLNILVRMIRAYVNGEYKMMPWKALVSLAAALIYFITPLDLIPDFIPV
                     TGFLDDFTVIIWVFRSFQSEIDEFIEWEKDSINTSGGE"
     gene            79912..80562
                     /locus_tag="VGA21_03335"
     CDS             79912..80562
                     /locus_tag="VGA21_03335"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX9649568.1"
                     /translation="MENKVAIIAGASGLIGGHLVQLLLNSDYESIISVSRRKLDLEHP
                     KLVQKIVNFDQLHTLENELAADHIFCCLGSTIRKAGSKEKFILYDYTYPVELATITRT
                     NGASQFHIVSSLGAKKKSLFFYNRVKGDVEEAISKMDFRSIHIYQPSLLLGNREEFRT
                     GEKMGEYISYLFYPLYGLIKNYKPVKASKVANIMFAMAQLDHPGLHFHYSKEIQSY"
     gene            80565..81017
                     /gene="dtd"
                     /locus_tag="VGA21_03340"
     CDS             80565..81017
                     /gene="dtd"
                     /locus_tag="VGA21_03340"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017732127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HEX9649569.1"
                     /translation="MITVIQRVSEANVAIEGKVTAQIGHGLLLLAGFEEADTEADLEW
                     MGSKIVNLRIFPDENEVMNKSIMDSGGDILVVSQFTLHASTKKGNRPSYSKAAKSEVA
                     IPLYNTFLNQLEKVLGKKVASGEFGADMKVYLTNDGPVTIIIDSKDSK"
     gene            81026..81355
                     /locus_tag="VGA21_03345"
     CDS             81026..81355
                     /locus_tag="VGA21_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013929040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HEX9649570.1"
                     /translation="MTLREAQMEVDKWVTTKGVRYFNELTNMTILTEEVGEVARIISR
                     KYGDQSFKKREQKANLDDELADVLWVLICLANQTGVDLTDAFKDNLNKKNKRDATRHQ
                     RNKKLKK"
     gene            81352..82230
                     /locus_tag="VGA21_03350"
     CDS             81352..82230
                     /locus_tag="VGA21_03350"
                     /inference="COORDINATES: protein motif:HMM:NF019533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HEX9649571.1"
                     /translation="MKSHQLLIIMILPFAGCQEKSGTINDVYVSEMESWKERRLEAVT
                     GAEGWATLIGLHWLSQGETTMGSDSSRTIILPAEVPAYLGSFFVQGEQVIFSAADNSL
                     ITVADSTITTIHIDSNRNPVFEWETFSWYVIKRSERLGLRMRNTRSPEVLNFKGLNYF
                     PLDPEWIVKARFELHEPPRTKIIQTVLDMTIEYPNPATLFFEYRGEEYSLEALDEGGD
                     KYFLIFSDETSAVETYGAGRYMYISKSPVDGYLTLDFNKCYNPPCAFTPYATCPIPPP
                     ENQLKISIKAGEKDYH"
     gene            82643..83650
                     /locus_tag="VGA21_03355"
     CDS             82643..83650
                     /locus_tag="VGA21_03355"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEX9649572.1"
                     /translation="METVYFLGIDIAKKKFDGALTVDGKAFHQVESGNTCKEIKALFK
                     DLKKKFSISYSQLVVCMEHTGIYCRPLLEFLTENHIKVCVESSIQIKRSQGMVRGKTD
                     KIDAQRISSYAYRNRENLRFWTPQRLIIQKLKAMLVTRERLIRVKTELYVPINECEEF
                     IEESIHQMMNKACQNTINRIERDLDNIEKMIDDLVTEDAQLNHQYQLATSVTGIGKIT
                     ALHMIVSTGEFTRIQEAKKFACYSGVAPFEHSSGSSIRGKTRVSKMANMTIKKLLHLG
                     AMSAARYDHELQAYYQRKVLEGKNKMSVINAVRNKLISRVFACIKNNRKYQKIYKHAL
                     A"
BASE COUNT        27179 a        15310 c        17797 g        23381 t
ORIGIN      
        1 ttactgttta aaggccgtat atcccggtcc aaggaaactg gcgagatgtt aattaggatt
       61 ttatcaattg attttattct tcattaattt tacttgacga aagatataat tgaccaatat
      121 gaataaggga aacaaagtaa ggtatgtaac caatactaaa atagattcgc taataagctt
      181 aatagatacg ctaatcggca ctattgattc aatcggaaaa agttttgaag gaaagtcgga
      241 atctgaatta ggaatactga aattagttta cttagatatt cttaaaagaa cagttcctaa
      301 tcttcattca atatgtattt tgttaggaca atacaaagct tacaatcaat ataaaatttc
      361 gatttttctt ttacttcgaa cttgcctttc agattcctta ctaggtttat atttatcaac
      421 ctaccgtatg gataaaaatt catttgaaaa tgaggtaaat gtaattgata gatcatactt
      481 tgtagcaatg aaaaaatttc tgaaggagga acctcgctat aaaataccag tcgcatctga
      541 tactgatcta actaaggaat ttgaaaataa tttttcaaat ttaagaaaag gccatccaga
      601 gttctttaaa aaagataaat ctcttaaaag taataaggag ctaagatcaa gaagtaagaa
      661 ggagttattt gcaattaaac gtggagaaaa acctgatatg agttattctc ctaccgacga
      721 gaacttttat cagaggctga ttaaatttag agctttaaag gcatatggat atatatactt
      781 attgataaag ttttttgccc aatatcacca ctatgctttt aaaacaaggt ctctaattga
      841 gagcaataga gaatttgaat ttaacaagat tgttttgtcg atatctttct tgtatgcaac
      901 aacagatacc tttggaaatc tccttgattt agataaaaag ttgacggctc ctctaaaaaa
      961 aagaattgaa gagtttttga catttaaaac taactcttag ccttggttgg tgttttgcgc
     1021 agggtaaatg gggtggattc accaaccaac ttacccaata gggactccta cggagtatcc
     1081 cactaaaaat ccacggctca tcactttttt tcttcttcac ttcgttgaca ccaacaactc
     1141 cctaacattc acattcaaca catttgagat ctgaaccagc gtctccaggg aaggctgcat
     1201 gtcgttagtg caccaacggg aaacggttgt agcatttttt cccagtttat cagcaagcca
     1261 tttgttagtc ctgccttgtt caactaacac aatcttaatt ctattaattg ccctttttgc
     1321 catcttgatg cacttataat taattagaat atccttttac atcaaatata tagtctgaga
     1381 atcaaacagt tagattgtgt aaaagactaa tagaatagcg ttattattaa ttaatatgtc
     1441 gaataaaata attttaatat atatttgaac tattgtatga accttttgta ttgttattga
     1501 aaactcaatt ccagccggct gaatgaactt aaaaaccgct aactatccac ataaaactcc
     1561 ctttcgtccc atggtagaga aaagcctggt catccggctc caggccttcc tcagaaacac
     1621 acaaaacctc ttttagaaaa tatgagatac gtagatctac cagaaagcga attccaaaaa
     1681 ctactacagc aaattgaaga agattgcgat gaatacggtg atgatacacc actgttaacc
     1741 cttcagcaag tcatactgga agaactacgc caaaagctcg aagaagataa aatgaaatgg
     1801 aaaaagttcc cggtctggaa cctctttaaa cgacaatgat ccacaaaaaa aagccagggc
     1861 tcatacgccc cggccttcat ttaaaacatg ataatttatt caatgtgcaa tgtatatatt
     1921 ttttgaatat ccacaataga aagattatcc caaaaactgg taatatattt tcgtctcagc
     1981 gacatcaata gattaataat tggacctcca aaaaaaggat aaatcccaaa actatacgag
     2041 tctcatacta ggcatcacat aaaactcaat ctcctgattg acaacacata tccaaattac
     2101 tcaataaaca ataaatccag caaagtttct ctgcccatat gaacacccac tttcacatat
     2161 acttcgatct ccaaagccgg taatagcgtg tatcaatatt tttcagaatt tcaaaaatag
     2221 cacccgccca ataatttcgg atgccaaatc cggatggttg gccgcgaaga cgttctgata
     2281 acaaaacttc tttattagtc atgttaagca caattacgtg aaaatacgcc tctgtgtcga
     2341 tcttattcaa tgactctgca atgaagacaa ttccaatccc gacttcacct tccaaatcat
     2401 aagattcaat aatttgccgg atgtcatcct ttctgaaata cttcggactg taggtcaaca
     2461 tgttgtcagt atcagctttg gcattgcgag ccataaccat attaatatta atttctaaat
     2521 ccggccggcg aaaaaattct tttaaattat acttatcggg ttcgttcatt agcagcctgt
     2581 tccagcttgg gaaataatca atctgcaaat ctccgggatc cttcgggccg tgcccgccaa
     2641 aatgagagaa gtctccaact aactgcacct gggaaaaatc aatacccagc caggcaaccg
     2701 gataattgtg tccggtaaaa aaatcttcaa tggtttgccc gtatcctgaa acacttacag
     2761 ttaaaaaagt gagtatggta attatctttc tcatgagtcg aatgattttc tggttctgat
     2821 gaatttccaa atcttaattt cataaaatta atttatcttg gcttaagatg atagtttttg
     2881 gttttaaata acggacaata tttacagatt gctgatggaa caatttaaaa agcgtgatat
     2941 caaattaaaa ggttgggata cggagaataa tctcttaatg cggctaaact ctattgattg
     3001 caaaaaagga ctcctgtaca aagaacattt tctgctttta caatacacag gtcttcttga
     3061 taaatctgaa aatgaggttt atgatggaga catacttcta atcgcttcaa aaaaatttct
     3121 tgtgttttgg gactccgagc gttctggctg gggactggca gaaaatccgc aattgaaaaa
     3181 taaaaaaccg ctgcttgcga gggatgtatc caaatcgacc agactctgca gcttcctcga
     3241 atccccgcaa tctttcgggt gacgggttac taaaccatac taaaccagga ttaatttaaa
     3301 atgtcaaccc caaaatggtg agagttactt ttaataacga taccgggaaa gaattggagg
     3361 attttgttat tcgtgatcta agagactcaa ttatcaggga actggaaggt ctaaaatgtg
     3421 ctaaatgtaa aaaggatagt caagtacggg taactcttag aaagaataaa ctaaacggga
     3481 tgagggtaac tgtgaatgcg tgctgtgagg aattcaatca agaaattagt gaagtcttgt
     3541 tactcaacca ccctgcctct cctagcccaa tttcttctca atatacttaa ccgcttcatt
     3601 tacggtttgc aactgttcag cgtcattatc cgggattcgg atattaaaag cctgctcaaa
     3661 ttccatcact aactccgcgt aatccaacga gtcaattccg aggtctttta taagattggc
     3721 gtctggtgtg acttctgtgg gtgcaatacc taatttttca accagaattt cagttacttt
     3781 aacgcgaacg tctagcataa agggtgagtt aataaatccg gtggtaaaac tataaaaact
     3841 ttacttattt aaaaatgtct cccgggttag aacttaagcg aaaattcagt cccttcatca
     3901 agttttgatt taacggttat tgaaccctga tgttgtctca ttatttgacg ggaaaggctt
     3961 aatccgatac cagacccact cttcttagtt gtgtagaaag gaataaatat ccgttgcaaa
     4021 gcttcttcat caattcctga accattgtct ttgaccgaaa tcacgggcct tcctttgtct
     4081 gttttatagg ctcgcatttc aatgatccgc tcgttttgtt catcgaatgc ctgaatggaa
     4141 ttcttaatca gatttatgag gacctgctca atcaattctt tatctgcatt tatttttaga
     4201 tcagccggct gcaaagattt aatgaaataa attttatttt gatccagttc tttttgcatg
     4261 agagtaccga tttcatccag catttgtgtg accttaatag ttttgagttt tggtttgggg
     4321 gtatgagtca aatttctaaa atcctggata aaccggatta gtccctggct tcgcttttga
     4381 atagtctgaa ctgcaagttg catatcttca aattgttccc cattcagttt ggaggttttt
     4441 atctgcgatt cgatttcaga ttcaacggtt gcagcaaggg attgaatggg tgtgatcgaa
     4501 ttcatgattt cgtgagttaa aactctcaca agattttgcc aggcttccat ttctttttct
     4561 tcaagttcgg aatgaatatt ttgcagactc acgagtttat attcttttcc tctcagattt
     4621 aattctatgg catacaccgc aagttgaatc aggtctcctc caacttcgag ttttattaag
     4681 tccctgccac ctgtttttaa tctcagaatg atatctacaa ggttttcatt caaagatttc
     4741 agttcacgaa tattaaccag gttagaaaca cgcaatactt tttttgcggc cgcattcatg
     4801 atctgaatct gtccggaatc tttctcaaag gttatcagtc cgattccaac gtgctgcaca
     4861 atatttttca tatattgata atccgcttct ttctcccggc gcactgattt aaacttcaat
     4921 atgattttat taaactcctg gttgatttta ttcagaggct ccgcatcttc agaagttgtg
     4981 aatgatgcgg tatcatcatc aaattcaata gagtcaagga atgaagcaat ttcggaattt
     5041 ttcttatcaa gaaatttaat aagcgatatt agctgaatca ctaaaagaaa taagaatagt
     5101 atgggagtaa gaaaaatgtt gtcacccccc agagtttcat aaagtaaaat agaagtggtt
     5161 attatcaaaa gaattctgat aataaccgtt gatttaaatt tcttagagtc catgcttttc
     5221 cagcctcctg taaagagaag ctcgtgttaa gcctaattct ttagcagctt ttgagatgtt
     5281 tccgtcaaat ttgtttatgg ccttaagaat aacctgtttc tcaacttcat ccaggttgta
     5341 attatcagat tgatcagttg tatttatttg tgcatgattc aggaagaaaa atgcgtccgg
     5401 attaattgta tctccatcgc tcataatcac cgctctttca atagcatgct gaagttcacg
     5461 tacatttccc ggccacgaat atttttgtaa tttttttatc gcgctttctg aaattcttat
     5521 cccgggcttt cgatattttt ttccatagtt ctgaataaag tgctccgcaa gcataggaat
     5581 gtcttcttct ctatcgcgaa gcggtggcag gtgaatttct acggtgttta ttctgtagag
     5641 caagtcttga cggaatttgt tttcttttac catttccagg attggcaagt tagtagcgca
     5701 aattaatcgg atgtctatag aaaccggctg gttagtaccc actcgaataa tttctctttt
     5761 ctgaagtaca ctgagcaatt ttgattgtaa aggaaggctg agatttccaa tttcatcaag
     5821 aaacaaagtt cccttgttgg cgatttcaaa tcttcccggt ctatcttcct tcgcatcggt
     5881 aaaagaaccc cttttgtgtc caaataattc gctttcgaac aaggtctctg taacggctcc
     5941 catatcaacg ccaataaata cattgtcttt tcttaatgaa cgctgatgaa ttgcccgggc
     6001 caccaactcc ttacctgtac cattctctcc caggattaac acattggctt cggtggcggc
     6061 cactttttca atcaggtcaa aaattttttt gatcgctgaa ctgttgccga gtatgtcttt
     6121 aaagggttgg tttatatctt cttcgagttg ctttttcgct tttctgagtt ggtccacttc
     6181 tctgtaagac tgcctcaatt taatcgctga tgataaagtg gccagaagct tttcattttg
     6241 ccatggctta agtatgaaat ctgttgcacc ttcctttaat gcttttacag ccatctccac
     6301 atcaccaaac gcggttatca acaccaccac cgcattcgga tctttgtggg ttatctcttt
     6361 cagccaataa aagccttcct tgccacttgt cgtgtccttg ctgaaattca tatccagcag
     6421 aatcacatca taagtgtcat tgttaagcag gaatggaatt ttcttagggt tcttttctat
     6481 gataacctga tgggcgtgtt ttttcaacaa aagtttcgct gccaacaaca catcttcatc
     6541 atcatcaata ataaggattt taccgagtgc tttttcttcc acgtctttaa ttcgggttta
     6601 caggttttct gaatttcaat aaaaattatg ccacctgatg taacttacac taagtcagca
     6661 tgttaataaa acaatttgat cagattgaag accttaacat tacaaagagt gtctcaaaat
     6721 cggacacaat ttagataaaa ttcaaataca aatggcgata attactaagg gatatttcct
     6781 gcaatcgggt ggggaaagaa ttaataaaga ttgtattttc attaatttaa agaaatatta
     6841 tattgcattg ttgttattgg atgaacaata attagtccta tttattttga gtttttgacc
     6901 ttgggggcat cgtttgaaca cgatgcctct ttttattgtc caaagttgct ctttcgttga
     6961 ttatttcgat atgtggcagt gattttgcgg tactttctta tctacacctc aaaattgctg
     7021 cctatgataa taacctctac gactgaacaa aaaaatccaa actaccatcc catcaaatta
     7081 gcgcttatcc agcatgacat tctgcaaaga aagttattca aaagattgct ggaatccctg
     7141 caaaggttcc aggaaatcag aagttattca acttgcgaag agttgattaa attatctgat
     7201 tccgaaaggc cggatgtaat tgttattgat ccggaatctc attttattgg tttgactgag
     7261 tccttcagaa aggttaaacg acaattgccg tcagcaaata ttgttatggt aattgacgat
     7321 gagagtgact tcgtatttga tgctctgaat tccggagttt gtggtttcat caggagggga
     7381 tcaacgaaag atgaaattct gaatgccgtg gttgaggcgg ctaacggggg agctccgctc
     7441 acatcaaaaa ttgccagaat ggttgtagaa tcattccaga aaaatctgga gacaccatta
     7501 actgcgcgag aaactgaagt aatggaattg ctgtcaagag ggaaaagtta ttcgataatt
     7561 gcggatgaat tatttattca taaagaaacg gttaaaagtc atataaaaaa catctacgta
     7621 aaacttaaag taaaaagcaa ggcggaggca attgacaggg cgatgaaaga cagacttatt
     7681 tagcagcctg gtaagttttt agttccaccc ttccggaggc ataaactcca taagagaagt
     7741 tatcaatcca atcgccaagg ttgatataag ttgatgattc gcttatttcg taggtgtcgg
     7801 gtttatgtct gtgcccgaaa atgtagtaat catgatgcgc ttctttttca agattgacac
     7861 aatattgaag caggcgatcc ttttcgtgag agatttcttt ttcacccgat ttcctgcttt
     7921 tgacggacca ataattggcg attctaaatc caagatttgg atggattctc gcaaaaagcc
     7981 attggcaaaa aggattgctg aaaaactttt taagaatctt atacccggta tctccctgtc
     8041 cgagtccatc accatgacca accagtagaa tgctgccatc aatttttaaa gtcatgttac
     8101 tttttacaac agggatattt aattctctcg ggaaataatc aaacatccac atgtcatgat
     8161 ttcccacaaa gaaataaatg tcaatcccgt aatctgccag ttgacttacc ttacccagaa
     8221 atcgggtaaa gcctttcgga attacatgtt tgtattcaaa ccaaaagtca aaaatatctc
     8281 caaggaaaaa gattgaatgt gcatcagctg aaatagattc cagccatttc accattaatt
     8341 tttctctttt taaactttcg gagtaatcag gactacccag atggagatcg gaaacgaaat
     8401 aaaccttctt gcctggaggt aaaggagaaa gatctttcac catctatgat tatcagggtc
     8461 aattttcaga tgattcttta tttatttctt ctgattcatc actactcaaa tgttccggct
     8521 tttctttatc gtcatccttt acaggtattt ccggcttcac attttcggca ggagttccgt
     8581 tcagggaatc tgacttttgg gtataagcct gataggtagt acttgtattg aaaggtctct
     8641 ttccaattag cctttccaaa tccgtttgga atataatttc cttttcaaga agttctgtcg
     8701 cgacgatatt taattgctct cttttgtctt tcagcagctc ttttgttcgg gcaaatgcgt
     8761 cagagattat tttttttact tcctgatcta taatttgggc agttgattct gaatagggtt
     8821 tggtaaaagt gtaatctgat tgtttggaat cataatagga aacatttcca atcttatcat
     8881 tcattccata aatagtaacc atgctataag ccattttggt tacacgttcc agatcgctta
     8941 aggcacccgt agaaatctta ttaaatacaa tttcctctgc ggctcttccg cccagtgtca
     9001 tacacatttc atcaaaaagt tgttcagtct ggtaaaggaa ttgttccttt ggcaaatact
     9061 gggcataacc aagtgctgca attcctctgg gaacaatgct taccttaacc agcggatctg
     9121 catgctcaag gaaccagcca gccactgcgt gccccgcttc atggaaagcc acaattttct
     9181 tttcttcagg agaaataatt ttgctcttct tctctaatcc acctataacc cggtcaattg
     9241 catcatggaa gtcttccatt tcaaccgctt ttctatcttt tctggctgca attagtgccg
     9301 cttcattgca gacattggca atttccgctc ctgcaaaacc tggtgtttga gcagctagtt
     9361 tcttaggatc aacatccttt gaaagcttta gtggtttcag atgtacttta aaaatagctt
     9421 ctcgcccggc aatatcaggc ttgtcaatac ttatctgacg gtcaaaacga ccgggtcgca
     9481 gtaatgctga atccagaacg tctggccggt tggtagctgc aagaataatt actccggaat
     9541 cagtgctgaa gccatccatt tcaaccaaca atgagttaag ggtattttca cgctcatcat
     9601 ttgaacctgg aacctgacct ctgccgcgtg ttcttcctat tgcatcgatt tcatcgataa
     9661 agactatgca gggcgctttt tcttttgcct gtttgaagag gtctctaacc cgggccgcac
     9721 ctactccaac aaacatttcc acgaaatcgg aacctgacaa agtaaagaat ggaacagctg
     9781 cttcccctgc aactgctttg gcaagcaacg ttttccccgt gcctggaggt cctaccagca
     9841 aagcgccttt tggaatttta cctcccagcc tggtaaattt tgcaggtgtt ttaaggaaat
     9901 caacaatctc tttaacctct tctttggctt catccagtcc ggcaacatta tcgaatgtga
     9961 tctttacctt gttctctgca tcaaagagag ctgctttaga tttcccaata ttaaatattt
    10021 gtccccccgg acctccgcct cctgtcattc ttctcattag gaaccagaat ccgaataaca
    10081 gcagcagcag gaacccccag gtagatatga attgagtgaa gtctgatttt tcttccgtag
    10141 tataggtaat ttgctgatcc cttggaagtt tgttcattac gtctttaaaa ttttcattaa
    10201 acacttctac tgaaggtatt tcatatctgt aatgaggtcc gctggaaacg ccaaaaggat
    10261 tactgctgtc taactcagtc tggtatttgg cgttttgcaa tgcgccgggc tttaatgtta
    10321 tttcaacata ttgttggttc ttgatcaata caactttctg aacatcattg ctaagcagca
    10381 tttcctcaaa ccggtaagga tcaatggtta ccattgaggt agatttatta agataagtta
    10441 ctcccaatat cactgccagc agtattacaa taatccaaac ctgataattt ggcttttgag
    10501 gtcctttcga catcattttt ttcttatcgc tcatcccagg tttatttcta aaatatcttt
    10561 ttcataacgg ttttcaatac cagaagtttt taagcaacaa tttcaagggc aacagcatct
    10621 ccccacaaat tttcaaggcc gtaaaaagtt ctcttatctt ttttaaaaat gtgcaccaca
    10681 acattgatgt aatccaacaa gacccattcc aggttcttct tcccttcaat gtggtaaggt
    10741 ctctcaaagc cctctttcag ggtcacccct tccactgaat cagaaattgc ttcaatctga
    10801 gtatctgaat tgccagaaca aatcacaaaa taatctgcca ctgagtttgg gacattgcgt
    10861 aaatccatca ctaaaatgtc ggctgctttt ttttcctgca tccccctcac aactatctca
    10921 ctcaacttat cggagggcag aggattattt tttctcctca ttcaatacct ttgatttcta
    10981 caaaactacg aaaatccttg tataaaaatc ctgccaatac actatttatg ggcaaaaagg
    11041 tcatttacgt gccaacttgt cattcaacga atttgctcgc tgagcaggtt ttggcagata
    11101 agggcaatta tgaaggcact gtggtaatca ctgatcacca gacaatgggc aggggacagt
    11161 ttgatagaga atgggaaacc gaaccgggga aaaacctgac gctttctgtt attttgaagc
    11221 caagctttct gcatcttgaa tcccagtttc ttctgaacat gttctccagt ctcgcggtga
    11281 aaaagactgt ttccttcttt gcgggatatt ctgaaattaa atggcctaac gatatactta
    11341 ttaataacag gaaaatatcc ggcattttaa taaagaatgt tctgaaagga aaaacgataa
    11401 ctaactcaat ccttggaatt gggctaaacg tgaaccagga aaagttcacc aatgaaaatg
    11461 cggcttctct ccggggaatg acaagaaagc aacatgattt atccgttgtt ttttcaagcc
    11521 tcatggaaaa ccttgaatct tattatttga agttaagaga atcaaaatgc aaagcactgg
    11581 agaatgaata tcataattct cttttcggcc tggaaaaaga aatccatttt gaatctgaag
    11641 gagagtttaa agggattatc aaaggggttg acaaaaaggg ccaattgatt gtggaagttt
    11701 cagggaaaaa tcaaaagttt aacctgaatc aaataaaaat acattccaaa ggctggttag
    11761 gagaaaagta gtgaatattg tatttttgca cgctttaaga acaaaaaact atcaacaatg
    11821 attgaaacac agcgtaaata caaagttaag gatgtaagtc ttgcagattg gggcaggcga
    11881 gaaataaaac tggccgaatc agagatgccg ggactcatgg caatcaggga agaattcgga
    11941 tccaaaaaac cgttaaaagg agcaagaatt gccgggtgtc tgcacatgac aattcaaacc
    12001 gctgtactaa tcgaaacttt gattgaattg ggtgcggaag ttacatggtc atcttgcaat
    12061 atattctcaa cccaggacca cgcggctgcc gcaattgctg cagcaggaat accggtattt
    12121 gcatggaaag gaatgtcgga ggaagaattt aactgggcca ttgagcaaac attatttttt
    12181 ggagaaggcg aggaccggaa gccgcttaac atgattctgg atgatggtgg tgatttaacc
    12241 aacatggttt tagataatta tccggaactc gtttcaggca ttaggggcat ttcagaagag
    12301 acaaccactg gcgttcatag attgcaagag cgtgaaaaaa atggaagctt accgcttccg
    12361 acaattaatg taaatgattc agttacaaaa tcgaaattcg ataataagta tggttgtaaa
    12421 gaatctcttg ttgatgccat cagaagggcc accgatatta tgatggcagg taaagttgcg
    12481 gtggtagccg gatatggtga tgttggaaaa ggatccgccg cgtctttaag aggtgctgga
    12541 gccagagtta ttgtaacaga gattgatcct atttgtgccc tgcaggcagc aatggatggc
    12601 tatgaagtta aaaggatgga aactgcagtt gccgaagcca acatcgttgt aacagctacc
    12661 ggaaacaagg acatcgttac aagtcaaata tttaagttga tgtctgataa aactattgtt
    12721 tgtaacatag gtcattttga caatgaaatt gatgttgcat ggcttaataa gaattttgga
    12781 tcaaccaaag aagagatcaa acctcaggtt gataaataca ccattgatgg caaggatata
    12841 attcttttag cagaggggcg attggttaac cttggttgtg caacaggtca tccatctttt
    12901 gttatgtcca attcatttac taaccaggtg atggctcaaa tggaactctg gaataatatt
    12961 aataaatacg aaaacaaggt ttatacattg ccaaagcatt tagatgagaa ggtcgccaaa
    13021 ttacacctgg ctaaaatagg ggttgagtta actacgctta cccctgaaca ggcgaagtat
    13081 attggagttg aagtcaatgg tccatttaaa caggaatatt atcgctatta atttttaccc
    13141 acataaaatc agctagccaa agttttgcac tttggctttt ctctttttca gccagaatga
    13201 ttttttgtct tatccgcaag taagtttcta ttgccagaaa cttaccagag aatgatccat
    13261 aatttttgcc acaaatagaa tttggtaaaa atgaaccagg aagtgatttc ttctttatag
    13321 ttaaataacc acagcgtatt aacttacaat cctgaattat ttcatcctaa aggtggccac
    13381 attctgaagt tatcgttcgg ttttaggtta tttttgattg caatgggaga atcaattcag
    13441 cagctaaaca aggccgggaa agataaatta ttcgaaatgg aacttttgcc acaccttagt
    13501 tctctctata attttgcgta tagtttaact tctaatcagg atgattcgga cgacctggta
    13561 caggaaacct tcttaaaagc ctataaattt ctaaattcct atcaatccgg aactaatgca
    13621 aaagcatggt tgtttcgaat attgaagaac tcattcatta atgattatag gaaatcttca
    13681 aaagaacctc ctaaagttga ttatcaggaa gtggaatctt attacaattc agacgatgtg
    13741 gacataaaga tgacaaccga cctaagggtg gatgcagtaa atgggatgat gggtgatgaa
    13801 gtggcaatgg ctctgaataa tctggatgtt gactatagaa caataataat tctctgtgac
    13861 ctggagggat ttaagtatga agaaatggca aaaattcttg atattccaat tggaactgtg
    13921 agatcaagat tgcacagggc tcggcagctt cttaaagaac aattgcttga gtattctcaa
    13981 aaaatggggt tcacaaaaag gaaaaactaa acatgactca cgaaatatca aggtggatta
    14041 agaaaaatct ggtaaccagg tatattaaaa ccgaatgtcc tgaaagaaga aagtgtctgc
    14101 aagctttgga ggaaattctg gataatgaag cgccggaaga tatggagtct gagtataaag
    14161 ctcatattga ccaatgctgg agttgttttc aggattataa actggaacgg gcgattcgac
    14221 agctaatcaa gatcaaactt gaaaggaaac ctgttccaga taaacttctg gaagttatta
    14281 aaacaagaat tagtgaatca gaatagatca ggcttcaaag gcaaagccat tattatatca
    14341 gccccctccg gatccggaaa aacttcattg gcaagaaatc ttcttaagtc ccgaaacgat
    14401 ttggatttct cggtttctgt ttgcaccagg cctccaagac tcaacgaagc agagggcgta
    14461 gattattatt ttatttcgaa tgaagaattc aaggagaaaa ttgccaaagg agaatttatt
    14521 gaatgggaag aagtgtataa tggaatgttt tacggtacgc taaaatctga aattcaaaga
    14581 atatggacca acaaaaagca tgtcttattt gatgtcgatg taaaaggtgc actaaaactt
    14641 aaaaagcact ttggtgcgga tgcacttgct atttttatta aggctccctc aatagaagaa
    14701 attgagcgaa gattaacaaa aagaatgact gagaattcta cgtcaatgaa aatcaggttc
    14761 gataaaataa aattagagct gactttcgaa aaagagtttg acgtaacagt attgaacaaa
    14821 gaaattgaag aggcgaacag aaatatattg tcaattgtcg agaaatatct tgcttcatga
    14881 aggttggatt attttttggt tcatttaacc ccattcatac aggtcactta attgtagccc
    14941 aggcagtact cgagcaaccc ggcatcaatc agatttggtt tattgtttct ccccgaaacc
    15001 cattgaagaa gaacgcgaac ctgcttcatg aattcgacag gctggattta gtaaatgcag
    15061 caatttggga taatccaaaa atgaaagcta aggatattga attcagatta ccaaagccca
    15121 actacacaat caatacaata attgctttga aggagaaaca ccctaagcac gaattcttcc
    15181 tcataattgg acaggataat cttagccaat ttccaaggtg gaaaaatcat aaagaaattt
    15241 tgaaggaagt acatttatat gtgtaccccc gtcccaatgc tcagaattct gaactcgata
    15301 aacattctaa agtaaaattc attgaagcgc ctctcttgga tatctctgca actttgatca
    15361 gaaaatctgt aaaaaaagga agatcgatta aatatcttgt tccggaaaca atagaggaaa
    15421 tgatcaggcg aaagaaattc tatctctgag ttagacgtga agtatgtccg tttctttatt
    15481 tgctatcaaa tcgtcaattt tttttgtgta cgtcgaagtc agctcatcta ttttttcttc
    15541 accttttcta atttcatctt ctgcaatgcc ttcatctttt aatgctttaa tcgaatgaag
    15601 tacctcatga cgtgcattcc ttatgctaat ctttccttgc tcgccttcat gtttagcctg
    15661 tttcactaat tgaaccctgc gttcttcggt taagattggt atattgataa taatctgctc
    15721 tccatcgttg tggggattca atcctaaatc agaattgata atagaccttt caatctctga
    15781 taccagactt ttctcccagg gttttataaa tattgatctt gcatcaggcg tgcttaatga
    15841 agcaacctgg ttaagaggcg ttgcattgcc gtaatactgt actctgatac tatccagcat
    15901 tgaggtcgtc gctttcccgg ctcttatttt tgcaagctct ccatttacat gttgcagagc
    15961 cttttccatc aaagatttcg cctcatctaa taacataata acttcttcca tggcttagtt
    16021 aattaaggtt ccaacttctt tgccaattat tacatcaagt aaattccccg gtttattcat
    16081 gtcaaaaaca atgataggaa gattattctc catacatagg gtgaaggctg tcatatccat
    16141 tatgttaaga ccctttgtgt atgcgtcttg aaatgttagg ctggtatacc gctctgcatc
    16201 aggaaatttt tcaggatcag ctgtgtaaac tccatcaacg cgtgtgccct tgagaaccac
    16261 attcgcttca atttcaatag ccctcaggct tgcagtagag tcggttgtga aataaggatt
    16321 tcctgtgcca gctccaaaaa taacaatccg gcctttttca agatgccgga ctgcccttct
    16381 ctttatgaat ggttcacaaa cctgatccat tcttatgcct gacattaatc tggtataaat
    16441 accttgctct tcaagggcgc tttgaagggc catcccatta ataacagtgg caagcatacc
    16501 catataatct ccctgcacgc ggtcaattcc catcctctca gcatcacttc ccctgaatat
    16561 gttgcctccc ccaataacaa ttgcaatctc aacccccatt tcactaacct tctttatctc
    16621 ttcagcgtat tgtagcaatc gcttgggatc aataccaaag gatcttgtgc ccatcagggc
    16681 ttcaccactg agttttagca gaattctttt atacttcatg ttttggaaag gaaatgatac
    16741 atttttttgc aaatataaga ctcaacctct tttaaaagtg cttaagcttt tttaaaattc
    16801 aatcaaaatc tgattctttg ccacgctttc tttttctttc gcctttatat ttttgatgat
    16861 tccatcaact ggagatttaa taatattctc cattttcatg gcttcaagta taaggatagg
    16921 ctcccctttc ttaatggaca ttccactttt caccagaatc tgcaaaatta acccaggcat
    16981 tggagctttg atttcaccaa cagaggcagc aatagttttg tacatcccca ttttattcag
    17041 caactcatca agggttgttc ttagctcgcc agaaataata ctattattaa cccttaagac
    17101 gggagtgttg gaattgccat taagggtgag tacttctaaa ttgaaagacc tgcaatcaat
    17161 cagtatatta tagtaataag ggtttattgg aagaatatta atagtcactt cggatccgtt
    17221 taagagccat tttccgtcaa acttttcaac gttgaactcc accttatcct gagattcgaa
    17281 cacaattgta tgcatctgca atttaagttg ggtgatttgc taaaatacga ataattccat
    17341 cgcttgaaaa ccaagaagat gccaaagaag ctcaacttca aaattgaatt caaaaatttg
    17401 aataaaataa atttaaatct atttttgcaa ctccaaaatt gagggtaacc agactatcct
    17461 ttttattagg aactgaaggg gggataccga agcggtcaaa cggggcagac tgtaaatctg
    17521 ttggcgcagc cttcgtaggt tcgaatcctg ctccccccac cagaaagcat tcggtagctg
    17581 gaatttggta atattatatt aaattcgcag tccgattatc ataagcggga gtagctcagt
    17641 tggtagagcg acagccttcc aagctgtagg tcgcgggttc gaacctcgtc tcccgctctg
    17701 tgaaggctta gtaaagccga tgtagctcag gggtagagcg cttccttggt aaggaagagg
    17761 tcaagggttc aaatcccttc attggctcgg ataaagtcgg aaaattttgt agaaataaat
    17821 agtaatcata aaatacactt aaaaattgaa ttaattaaaa ttaggaattt tcaaatatgg
    17881 ctaaagaaac ttataaccgc tcaaaaccac acgtgaacat tggaactata ggtcacgtag
    17941 atcatggaaa aaccactctg actgctgcca ttaccaaagt tctttcagcg aaaggcttgg
    18001 cccaaataag agacttttct tcgattgaca acgctccgga ggaaaaggaa agaggtatta
    18061 caattaatac cgctcatgtt gaatatcaga cagacaaaag acattatgct cacgttgatt
    18121 gtccaggtca tgctgactat gtgaaaaaca tggtaacagg tgccgctcaa atggacggcg
    18181 ccattctggt tgtagcagca acagatggac caatgcctca aaccagagag cacatcttat
    18241 tggcaagaca ggttggagta cctgctttag tggtgtttat gaataaagtt gaccttgttg
    18301 acgatgagga attgttagac ttggttgaaa tggaagtgag agaactatta acattttatg
    18361 aatttcccgg agactctatc cctgtaatca ggggttctgc attaggagga ttaaatggcg
    18421 aagctaaatg ggtagaaaaa atccaggaat tgatgaatgc cgttgatgag catattccaa
    18481 taccagagag acttatcgac aaggacttcc ttatgccgat tgaagatgtt ttctcaataa
    18541 ctggtagagg tactgttgct accggtagaa tcgaaagagg agtaatcaat tccggcgatg
    18601 gcgtagatat tcttggaatg ggtgcagaaa acctgaaatc caccgttact ggcgtagaaa
    18661 tgttccggaa aatccttgat agaggcgagg cgggtgacaa tgtcggtcta ctcctccggg
    18721 gaattgaaaa ggatgaaatt cgaagaggaa tggttatctg taaaccaggt tctgtaacac
    18781 ctcattcact cttcaaagct gaaatctacg tattatcaaa agaagaaggt ggaagacata
    18841 cgccattctt taataagtat aggccgcagt tttatctccg aactactgac gtaacgggtg
    18901 aaattaaatt gcccgatgga gttgaaatgg tgatgcctgg agataatgtt accattgaag
    18961 ttaacctaat taacaaggtt gcaatggaga aaggacttcg atttgcaatt agagaaggag
    19021 gcagaacggt aggggccggt caggtgaccg aaatccttga ttaaggcagg ggaataagct
    19081 ctttcaatag ttagtataac agacgcgttt ctgcataaaa aagtgcgaaa cgcgtttgtg
    19141 ttatagcata tattatgata cctttgcagt cccttttggg ttgctcaaag acgggtgtag
    19201 ctcaattggt agagtggtgg tctccaaaac cacaggttgg gagttcgagt ctctccaccc
    19261 gtgcaagtaa aaaccggtaa tgatgcagaa gctaattgag ttctttaaag agtcgtatga
    19321 cgaaatgaga tttaaggttt cctggccaaa atacagggaa cttcaaagta gctcggtatt
    19381 ggttttggtg gcctccttaa tattcgctct aatcatagga gtaatggatt tggggtttga
    19441 aaatatattg gactggtttt accgcgaatt ctaaaatagc atggagatga ctgaagtgaa
    19501 gtggtacgta atccgtgtct taagcggtca ggaaaagaag gtaaaggctt acctggaaac
    19561 tgagattgaa cgggaaaatc taaaggaata cattcccgaa gtaatgattc cttcagagaa
    19621 ggtatatgaa atgaggaatg gaaagaaaag ggttcgcgaa agaaatttct ttcctggtta
    19681 tgtattggta caggctaatt tggaatatgg agaagcaaaa catgttgtca taagcattcc
    19741 gggagtaatt ggttttctcg gagcaaacag cacaagtact tcgaaagaac cggttcccct
    19801 gagacaaagt gaaataaaca ggattctggg taaggttgac gaggtggatg aaatggttga
    19861 aaagctggat cgcccatata ttgttggtga aggagttaaa gtaatggatg gtccgtttag
    19921 cggttttacc ggaaccgttg aggaagtttt tgaagaaaga aagaaattaa acgtaatggt
    19981 taaaatcttc ggaagaaata cacccgttga acttaattat atgcaagttg aaaaattaga
    20041 ctaaatggct aaggaaattg aaggtttttt gaaattacag gtaatgggcg gctcggctaa
    20101 cccatctcct cctgtaggac cagcattggg tagcaaagga ctcaacatca tggatttctg
    20161 caaacaattt aacgcaagaa cccaggataa gcagggtact ttgatccccg tcctgatcac
    20221 aatatataaa gataaatcct tcgatttcgt tgtaaaaact ccgccggctc cggttttgat
    20281 actgaaggcg gctaatctga agaaaggtag tgctgaaccc aacaggaata aggttgggtc
    20341 attaacctgg aatcaatgta aagaaattgc cgaaaccaaa atgcaggatt tgaatgcgtt
    20401 tactattcat tccgcaatga aaatgatagc gggtactgca agaagcatgg gtataactgt
    20461 aaccggaact cccccgaggg aagctaatgt taactaggat gacaaagctg acaaaaaacc
    20521 aaaaaaatgt gcttgagaat tttgatccaa tgaaatctta ttctctggag gcagcatcaa
    20581 aactggtaaa ggaaatcacc actaccaaat ttgattcatc agttgacctg gacattcgcc
    20641 tcggagttga tccgcgcaaa gccgaccaaa tggtgcgcgg agtagttgct ttgccccatg
    20701 gaaccggtaa gcaagttaaa gttcttgctt tggttactcc ggataaagag aatgatgcca
    20761 aagaggccgg tgcagattat gcgggattag atgatatcat aaaaaaaatt gaaggaggat
    20821 ggatcgattt cgatattatc gttacaatgc caactgttat ggcgaaagtt ggtaagctgg
    20881 ggaaaatcct gggaccaaga ggccttatgc caaatccaaa agccggtacg gtaactcttg
    20941 atgtcgcaaa agctatcagg gaagtcaagg ctggtaaaat tgatttcaaa gtagacaaat
    21001 tcgggatcat tcacgccagt attggaaaag tctcttttac ggaagataaa attcttgata
    21061 acgcaagaga gcttattcac acaatttcca aacttaagcc ggcatcttca aaaggcactt
    21121 acttcaggag cataagtctt tccagcacaa tgagtgcagg aatttccatt gataaaagtt
    21181 caattgttgg aatttaaaaa gtcatgatac gagaagaaaa agcaacaatt attaatgaac
    21241 tctcagaaaa gctgaaggaa aataacaatt attattttac agatgctaca ggtatgaccg
    21301 ttgctcagat aaatgatttc aggagaatgt gtcacaaggt tgacattgat tacaaagtgt
    21361 ataaaaacac tttaattatc aaggcactgg acacccttaa atttgattat tccccgcttt
    21421 atgaaaaagc ccttaagggt gcaacaggaa ttttgttttc aaaggaaatt gccaatactc
    21481 cggctaagtt gattaaggaa tttagaaaaa agagcaattt ggacaagcct ttattcaagg
    21541 gggcatatat caatcaggat gtttatatag gcgagaatca actggatact ctaagtaatc
    21601 tcaagtccag ggaagaactt attggtgaga taattgccat tctccggtct cctgctcaaa
    21661 atgttatttc agcacttcaa agtggaaaga ataaacttgc cggtatcgtt aaaactttat
    21721 cagaaaaaac aaattaataa atcattaaag caaatatcta aatcaaacta aaaatggcag
    21781 acttaaaagc attcgctgat cagcttgtga acttaaccgt gaaagaggtt aatgagttag
    21841 caacaattct taaggaggag tatggtattg aaccggcggc agcggcagct ccagtaatgg
    21901 ttgctggtgg aggcgcggca tctgcagaag gtggagcgga agaaaaatca tcattcgatg
    21961 taattctaaa atctcctggt ggagccaagc tggctatcgt gaaactcgtt aaggaactga
    22021 caggacttgg gttaaaagaa gccaaagacc tggtagatgg tgcaccgaaa gcggtaaagg
    22081 aaggattggc taaagacgaa gctgaaggct tgaaaaagca attagaagaa gcaggagcgg
    22141 aagtagaatt aaaataaaat ttggacaacc tgctgaacgg tagtctaact gcagttagac
    22201 cctgattttc gtcggggtct tttgctgttt gtagatgttt cagttttcta tgaataaaac
    22261 atttttttaa actaaataaa aattgccttg gcaactaaaa aatcaaacag aataagtttc
    22321 tccaacataa aatctgtaat tgattatccc gattttctgg atgttcagtt gaggtctttt
    22381 aaagactttt ttcagattga aaccccagcg gagaaaagaa cgcaggaagg gttgtacaaa
    22441 gtattttgtg agaattttcc tatcagcgat tcccgggaaa attttgtact ggaatttatt
    22501 gattacttaa ttgatcctcc caaatactcc gttgatgaat gcattgacag aggattaacc
    22561 tattcagttc ctttaaaagc aaagcttcga ctttcttgta atgatgaaga taatgaagat
    22621 tttgaaacag tagagcagga agtcttttta ggaaatattc cctacatgac cgagaaaggt
    22681 tctttcgtaa tcaatggagc agaaagagtt attgtcagtc agcttcatcg atcaccagga
    22741 gtattctttg cccaaagcaa acatacaaat ggtacaaagc tgtattcagc tcgaataatt
    22801 cctttcaaag gatcctggat agagtttgct accgatgtca acaatgtgat gtatgcctac
    22861 atcgacagaa aaaagaaatt cccggttacc acacttctca gggcaatcgg atatgggact
    22921 gataaagata ttcttgattt gtttggattg tctgaagaag tttcagccac aaaggcaaat
    22981 cttaaaaaag ttgtaggaag gaaattggcg gcacgtgtcc ttaaaacctg gactgaagac
    23041 tttgtggatg aagatactgg cgaggttgtt tctattgaca gaaacgaagt attgcttgag
    23101 cgggactcgg tgctaacaaa agaagatgtc gaactgataa ttgaatccga tacaaaatca
    23161 attattctcc atcgggatga tgtcaacatc acagactaca atatcatttt caatactctg
    23221 gctaaagaca attcgaactc agaaaaggaa gcggttgagc aaatttatcg gcagttaaga
    23281 aatactgaag cccccgatga acagactgca agagatatta tccaaagtct tttctttagt
    23341 gataagagat atgatctggg cgaagtagga cgatacagaa ttaataggaa actcggaatt
    23401 gatataggat ttgagcagcg agttctaaca aaaggtgata ttatccttat tgttaaatac
    23461 cttattgggt taattaactc taaggcggtt gtagatgata tagatcacct gagcaacaga
    23521 agggttcgaa ccgtaggtga gcagctttat acacaattcg gaattgggtt agccagaatg
    23581 gccagaacca ttaaggaaag gatgaatgta agggataacg aggattttaa accagtcgat
    23641 ctcataaacg cgagaacact ttcctcagtg attaattcat tcttcggcac aaatcaactc
    23701 tctcaattta tggaccagac aaatccattg gctgagataa ctcataaaag gagaatgtct
    23761 gcgctaggcc caggtggtct ctccagagaa cgcgcaggat ttgaagttcg cgacgtgcat
    23821 tacacccatt atggaagatt gtgtaccatt gaaactcccg aaggaccaaa catcggtttg
    23881 atctcttccc tttgtgtaca cgccaaggtg aatcatatgg gattcattga aacaccgtac
    23941 cgtaaagttg agaagggact agtttcaacg aatgtgattt ttcttactgc tgaagaagaa
    24001 gacactcaca acattgctca ggcaaatgct aaaattgatg acaaaggtaa attcgttaat
    24061 aaaagagtaa aggcaagata tgaaggagat tttcccgtcg tagagcccaa aaaggtttct
    24121 tatatggata ttgctcctaa ccagattgtg tctgttgcgg cttcgcttat tccattcctg
    24181 gaacatgacg atgcaaacag agcgctcatg ggatcaaata tgcaaagaca ggccgttcca
    24241 ttgatgaagc cggaagctcc cattgtggga accggccttg aaagaagggt agccgaagat
    24301 tcaagagcgt taatcctcgc tgaagatgag ggagttgtgg aatattctga tgccactaaa
    24361 ataacaatca ggtacaagca aaccgcggaa caaaaatcca ttgcctttga agatgatctc
    24421 aagatttatg acctggtcaa attcagacga actaatcagg atacctgcat caaccttaag
    24481 cctattgtta agaaaggtga tagtgtgaaa agtggaacac cactttgtga aggatatgcc
    24541 acgcaaaatg gtgaactggc tctgggaagg aatttgcagg ttgcattcat gccctggcaa
    24601 ggatataatt ttgaagatgc tattgttata tccgaaagag ttgtgcgaga tgataccttt
    24661 acttctattc acattgaaga atttgaactc gaagtaaggg acaccaagag aggagaggaa
    24721 gaattgacct ctgaaattcc aaatgtgagc gaagaagctg taaagaattt agatgaaaat
    24781 ggaataatta ggattggagc ggaagtaaaa gaaggagata ttcttatagg gaaaattact
    24841 ccaaaaggag agacagatcc aactccggaa gaaaaactat tgcgggcaat ttttggtgat
    24901 aaagccggcg atgtaaaaga tgcatcattg aaggcgcccc cttctcttag aggagtcgtt
    24961 attgaaacaa aacttttctc cagaccaaaa aaagataaag atttaagatc caaatcaaaa
    25021 aaagatgtag aaatactaaa gtcaaaatac agtaatgatt taagaacatt aagggcagag
    25081 atgatagata agctcgctgc tctgctgcat ggaaaggtgt ctgaaggagt aaatcacaaa
    25141 tttggcgaat ccctgatttc aaagggaaca aagtttaccg caaaagtaat ttcaaataat
    25201 attttccctg aaaagaatgt tttcagagac gaaagtaatt ataatgttcc tgaggaagta
    25261 aatcttatta gcgatataaa tcttgatggg tggaccaaag attcgaaaac caacaagttg
    25321 atcgaaaggc tggttaaaaa ttatatggct gcccgcaata aaatagctgg tgaattcaaa
    25381 agagaaagat ttacacttga agttggtgat gaactccctg ctggaattgt tcaattggct
    25441 aaagtgtata ttgccaagaa gcgtaagctg aaagttgggg ataaaatggc aggtcgtcac
    25501 ggtaataaag gtgttgtggc taaaatcgtg agagaagagg acatgccttt tcttgaagat
    25561 ggaaccccga tggatatagt gctaaatccc ctgggtgtgc cttcaaggat gaacctgggt
    25621 cagatttatg aaaccgtgct tggttgggct ggaatgaagt tagatcgaaa atatgccaca
    25681 ccaatttttg acggtgctac tattgaagaa gtttccagtg aattgaatga agctaattta
    25741 ccagagttcg gccggaccta tttatatgac ggattaagcg gagataagtt tgaccaacaa
    25801 gttacagtag gtgtgattta tatgcttaaa ctgggacacc ttgttgatga taaaatgcac
    25861 tccagatcaa ttggacctta ttcactgatc acacaacaac cacttggcgg aaaagctcag
    25921 tttggcggtc aaagattcgg tgaaatggaa gtatgggccc ttgaagcttt tggagcggcc
    25981 aacgtcctcc aggaaatcct aaccataaag tctgatgatg taattggtcg cgcaaaggct
    26041 tatgaagcta ttgtgaaggg agagaatatg acaaaaccca atatccctga atcattcaat
    26101 gtgttggttc atgaattaag gggccttgcc ttagaaataa cattagaata agaaattata
    26161 aatcataaat aaagccattt aaatatgtct ttcaggaaaa ataaaaaaat taacactgat
    26221 ttctcaacag ttactataag ccttgcatct ccagaatcaa ttttggagac ctcacacggg
    26281 gaggtaactc agccagagac cataaattac cggacctaca agcctgagat gggaggactc
    26341 ttctgtgaga gaattttcgg gcctgtaaaa gattgggaat gccattgtgg caaatacaag
    26401 agaatcagat acaaaggaat catttgtgat cgttgtggag ttgaagtgac agagaaaaaa
    26461 gtccgccggg agcgaatggg tcatattgag ttggtagtcc ctgttgcaca tatctggtat
    26521 tttaggtcct tacctaataa aataggctat ctggttggac tccccaccaa gaaactggat
    26581 caaataattt attacgagag atacgttgtt attcaggcag gaataaaagt tgaagacggc
    26641 gtttcatatc tggactacct gaccgaagat gaatatctgg acatactgga taagctgccc
    26701 agggaaaatc agctgcttga agattctgat ccgaataagt ttatcgcaaa aatgggagct
    26761 gaagctctcg aaatgctcct tagcagacta aacctggatg taatgtctta cgatttaaga
    26821 caccaggctg ctaccgacac aagccagcaa agaaaagctg aagctcttaa gagattgcgc
    26881 gtggttgagg catttagaga tgcaaataca aggatagaaa accggccgga atggatggtc
    26941 ataaagatgg taccggttat tcctcctgag cttcgccctc ttgttccttt ggacggaggc
    27001 agatttgcaa cctctgattt gaatgactta tacagaaggg taatcattcg caataacagg
    27061 cttaagagat taattgatat taaagctccc gaagtaattc ttcgaaacga aaaaagaatg
    27121 ctacaggaag cagtagatag tttgtttgat aactcacgaa aggtaaatgc ggttagatct
    27181 gatggaaaca gagcgctgaa atccttgagt gatatgctga aagggaaaca aggcagattt
    27241 aggcaaaacc tccttggtaa aagagtagat tattctggtc gttcagtaat agttgtcgga
    27301 cctgaattga aacttcatga atgcggacta ccaaaagata tggctgcgga attatttaag
    27361 cctttcatta tccggaaact tattgaaaga ggaatagtta aaacagtaaa atccgctaaa
    27421 aaaatcgttg atcgtaaaga tccggtagtt tgggatattt tagaaaacgt tcttaaagga
    27481 catcctgtat tgcttaatcg tgctccaaca ttgcaccgat taggtattca ggcatttcaa
    27541 cctaaactaa tcgaaggcaa agctatccag atccatcctc tggtgtgtac agcatttaac
    27601 gccgattttg atggtgacca aatggcagtt catgttcccc ttggccatga agcaatcctt
    27661 gaagcatcgt tattgatgct ttcatctcac aatattttga atccagctaa tggtgctcct
    27721 attacagtac catcacaaga tatggtactt ggcctttatt acattacaaa aggtaggaaa
    27781 ggaacaaagc aaagtccggt taaaggagaa ggattatctt tttatgaccc aacggaagtg
    27841 ataatcgctc ttaatgaggg aaggctcaac aaacacgcta acattaaagt ccgagtaaaa
    27901 ataagaaatg aaaaagagga actggagatc aaagtgattg aaaccgtcgc agggcgagta
    27961 atttttaatc aatttatacc tgatgaagtg ggatatgtaa atgaattgct taccaagaaa
    28021 aagcttcaga aaattatctc tgacgtacat aaaattgccg gaacagccaa ggccgcaaaa
    28081 tttttggatg atattaaaga tcttggattc caatcagcat acaaaggcgg attatcaatg
    28141 ggactgaacg atgtaaaaat tccggagtcc aagaccaaac taatcgagca agcaaagaaa
    28201 gaagtagatg ttgtctggaa taattatatg atgggtttaa ttactgataa tgaaagatac
    28261 aatcaggtaa ttgacatttg gactcgaatt aattcccaaa ttaccaacgc cctaatgact
    28321 cagttggaag aggatgatca gggattcaac tccatataca tgatgatgca ttccggtgcc
    28381 cgcggttcaa gagaacaaat caggcagctg ggaggcatga gaggtttaat ggcaaaacct
    28441 cagaagaacc ttcagggatc agttggtgaa attattgaaa acccaattct atcaaacttt
    28501 aaggaaggat tggatgtaat agaatacttc atttcaaccc atggggccag aaaaggtctg
    28561 gcagacacgg ctctgaaaac ggcagatgct ggttacctta cgcgacgatt ggttgacgta
    28621 gcccaggatc ttgtaattaa tgaagcagat tgcggaactc tgagaggact ggtggttaca
    28681 gcacttaaag ataatgaaga catcgttgaa ccgctgtcag aacgaatatt gggtcgtgtt
    28741 agtgtgcatg atatatatga tccattaaca gaggaattga ttgtaaaatt tggtgaagaa
    28801 atcactgaag agattgcaaa aaaaattgat gaaaccagca tagaggaggt tgaaatccga
    28861 tcacttttaa cctgcgaaac aaggaagggc tcttgcgcta aatgctacgg tagaaatctg
    28921 gctacaggaa gaatggtaga aagaggcgaa gctgttggag taattgctgc tcaatccatt
    28981 ggagaacccg gcactcagct gacactccga actttccacg ttggtggaac cgcatcaaac
    29041 attgcagtgg aagccagaat tcgcgctaaa ttttccggca ccgtagaatt cgaagatatc
    29101 aggtcagtta caaccaaaaa ttctgaagga gaaaaggtag aagttgtgat gggtcgatct
    29161 ggcgaaataa gaattgtcga taaaaagtct aaaagagttt tgatgaccaa tcacgttccc
    29221 tacggagcat tccttgtagt taagagcggt gtaaaagttg aaaaaggtga tgaactgtgc
    29281 ttctgggatc catataacgc cgtaatcctg tcagaagtag aaggaacgat tgattttgac
    29341 gcgattgaag aaggtataac ctttcgtgaa gaaagtgatg agcaaactgg tcacagggaa
    29401 aaagttatta ttgatacaaa agacaaaacg aagaatccct ctattgtcat caagaaagga
    29461 aaagaagtga agtcttacaa tattccggta ggcgctcacc tggtagttga tattaaggat
    29521 aaagtaatcc caggacaggt tttggcaaaa attccaagaa taatgggcaa gtcaagagat
    29581 attaccggag gtttgccgcg ggtaacagaa ttattcgaag cacgaaatcc ttcaaacccg
    29641 gcggtagtaa gtgagattga cggagttgtc acatatggtg ctataaagag agggaacagg
    29701 gaaatcttca tagaatcaaa agatggtgtt aagaagagat atttggtttc tctttcaaag
    29761 catattcttg ttcaggataa cgactatatc agggcaggag atcctctgtc tgacggagcc
    29821 attactcccg ccgacattct tgctatcaaa ggtcctacag ccgtacaaga gtatctcgta
    29881 aatgaaattc aggaagttta cagacttcaa ggtgttaaga taaatgacaa acacatagag
    29941 gcgattgtga gccaaatgat gcagaaagtg gaaattattg aggctggtga taccaatttc
    30001 ctccaggggc aaacggttga taaatttgcc ttccaggaag aaaatgattc tgttcttgat
    30061 gtgaaagtag taactgaccc tggtgattca gaatcattaa aagcgggaca aattgtttcc
    30121 tcacgaaaat tgcgtgacga aaactccagc ttaagaagaa gagatctaaa aattgttgca
    30181 gttcgtgacg cagaagcagc cgtaagccgt ccaacattgc agggaatcac ccaggcatca
    30241 cttggaacgg aaagcttcat ttcggcagct tcattccagg aaacaaccaa agtgctgagc
    30301 gaagcatcaa tccgtggtaa aatagatctc ctcaatggat tgaaggaaaa cgtaattgtt
    30361 ggtcatctta ttccggccgg aactggtcag aaaagattca agaatcttat cgttggttct
    30421 caggaagagt atgacacatt aatagcatcc aaggaagaat tcaatagaga aaaggctgaa
    30481 gaagtaactc aggattaatg gcagattcaa aagaaaataa agcaaagcaa aaccaaatca
    30541 acatagagct ttcagaagaa atcgctgaag ggatatatgc gaatttggcc atgattgccc
    30601 actcaaatag tgagtttgtg atagatttta tccgcttaat gccaggagta cccaaagcaa
    30661 aagtgaaatc cagaattgta attactccgg aacatgctaa gcgactcctt catgctcttg
    30721 gtgagaacat ttcaaaatat gaagacacct ttggccctat taagaaaacg gatgaaaccc
    30781 caaaatttcc aataaatttt ggaggcactg ttggagaggc ttgagtaaaa ataattcggt
    30841 cagtcggtgg ttgcatttcg tgtttactgc agctctgttt tgtaaataaa tttgaattat
    30901 gaggcttgta aactagcatt taaagctata cctttgcaat ccgaaatttg ggcatttatg
    30961 cccgtttttg attataaata aaagattctt taatgcctac catacaacag ttagtaagaa
    31021 agggaaggaa aaagctgacc tcaaaatcga agtcacctgc gcttgattca tgtccacaaa
    31081 agagaggagt gtgtacccgc gtttacacca ctactcccaa aaagccaaat tccgcgatga
    31141 gaaaagtggc cagggttaga ttgacaaatc aaaaagaagt caatgcctat ataccaggtg
    31201 aaggacataa cttgcaggaa cactccatcg tgttgatcag aggcggcaga gtaaaagatc
    31261 ttcccggagt aaggtatcat ataattcggg gcgctctgga taccgccgga gtaagcggca
    31321 ggttgcaaag aagatccaag tacggtgcca aaagacccaa gaagtaattt attgagtaat
    31381 gagaaaagca aaaccaaaga agagatacat tctgcctgac ccaaagtacc atgatacttt
    31441 ggtttctaaa tttgtgaact gtttaatgat tgagggcaag aaaaataaag cgtacaatat
    31501 tttctatgga gctatagatc tggttgaaga taaattgaaa gaaaatggtt tagaggcctg
    31561 gaaaaaagca ttgaataata taacgccctc agttgaggtt aaaagcagga gagttggagg
    31621 tgcaactttt caggttccac ttgaagttag accggaaaga aagatatcat tgggcatcaa
    31681 atggctaatt aaatactcag aggctcgcgg agaaaaaaca atgaccgaaa agttagccgc
    31741 agaaataata gcggcaacca aaggtgaagg tggagcggta aagaagaaag acgatactca
    31801 tagaatggct gaagccaata aggcattttc acacttcaga ttataatcaa tggccaagaa
    31861 agaactcaga agattaagga acattgggat tatggcccat attgatgcag gtaaaacaac
    31921 cactaccgaa agaattctat attataccgg attaagtcac aaaattggtg aagtacatga
    31981 tggcgctgca ataatggact ggatggagca ggaacaggag cgtggaatta ctatcacatc
    32041 agcagcaact actacctcct ggaagtatcc aactgatcaa ggtcatacag tagataagac
    32101 cgaggagtat atgattaata taatagatac tccaggtcac gtagacttca ctgttgaggt
    32161 tgaacgatcc ctaagagtat tggatggcgc tgtggcatta ttttgtgctg tctcaggtgt
    32221 ggaacctcaa tctgaaacag tatggagaca agcggataaa tataaagtac ccaggatttg
    32281 ttttgtaaat aaaatggaca gatctggtgc tgatttcttc aacgttgttt ctgaaatcaa
    32341 atccaaactg ggagcaaatc ccatcccttt acaagttcct attggagcag aagaaacttt
    32401 caaaggagtg gtagacctca ttaccgataa ggcaattgtg tggaacgatg acgatttggg
    32461 aatgacatat aaagtgattg atcttcccga agatcttaaa gaaaccgttt ttgaatggaa
    32521 gcaaaaactt gttgaaaatg tagcggaata cgacgatgtg attctggaga agttcttcga
    32581 taacccaaga tcaataactg aaaaagaatt aagagcagct atccgcaaag cggtgataga
    32641 tatgaaaatc tatcctgtct tctgtgggtc agctttcaaa aataaaggtg ttcaggctgt
    32701 gctcgatgct gtttgttctt acttaccatc acccattgat ttgccccctg taataggggc
    32761 taatccatat tccggtaagg aagaagaaag aaagccggat gccaatggtc ctttcgccgc
    32821 tttagcattc aagattgcaa cggatccatt cgtgggcagg ctttgctttt tccgtgttta
    32881 ctcaggtaca cttaatgcgg gttcttatgt tttgaacatg cgaacagaaa aaaaggaaag
    32941 aatttcgcgt ttaatgcaaa tgcattcaaa caaacaaaac ccgatagatg ttgttgaagc
    33001 tggggatatt tgtgcgggtg ttggatttaa agatattaaa accggagata ctttggttga
    33061 tgagaaacat ccagttgttc ttgaatcaat gagtttcccg gaacctgtta tcggttacgc
    33121 aattgaagcc aggaagcaag cagatgtaga taaacttagt attgcaattt caaaattagt
    33181 ggaagaagac ccaactttaa ccgttatgac tgaccatgaa acaggtcaaa cggttttaaa
    33241 aggtatgggc gagcttcacc ttgaaattat tcttgacaga cttaaaagag aatttaaagt
    33301 tgaggttaac cagggggctc cacaagttgc ctacaaagaa gctttaacga aaagaataga
    33361 acataaagaa gtttataaga agcaaaccgg aggtagagga aagtttgctg atatctcatt
    33421 cgagattgga cccagggaag ataacattcc gggattaaaa tttgaaaatg caatagtagg
    33481 tggcgcaata cccaaagaat ttatccctgc tgtccaaaaa ggatttgaac aggcgatggt
    33541 taatggtcca ttggcaggct atcctataga ttccatgcat gtaagattat ttcatggttc
    33601 ctaccatgac gtggattctg actccttatc ttttgaaatg gctgcccgga ttggctttaa
    33661 ggattcgtcg aaaaaagcat ctccaatcat actcgagccc attatggctg tcgaagtagt
    33721 tacacctgat gaatatacag gacccgttac cggtgacctc aataaaagga gaggaataat
    33781 gaaaggaatg gattccaagg gtggagctaa agttattaaa gcggatgttc cattatcaga
    33841 acttttcggc tacgttaccg atttgagaac catctcatcg ggcagagcat cggcttcatt
    33901 gacattctca cattacaatc cggttccaaa gaacctgtcg gaatctataa tagaaaaggt
    33961 aaaaggtgca ccagttaatt agcaatcaaa atgaaccaga aaattcgaat taaattaaag
    34021 tcttacgacc acaaccttgt ggataaatcc tctgaaaaaa tcgtaagagc agttaaaaca
    34081 accggtgcaa tcgtaagtgg acccattcct cttccaaccg aaaaggaaaa gtttacagta
    34141 ctgcgttctc cacacgtaaa taaaaaatcg agggaacagt ttcaactttg tacatataag
    34201 cgtttggttg acatatattc aaacagtgca aagaccgttg atgcgttgat gaaacttgaa
    34261 cttcccagcg gggttgatgt agaaataaag gtctgataaa aacaatattg taaatcaaag
    34321 agaaccaatc agttaagctt ggttctcttt tttatgggct aaaacaaatt taaaaggttc
    34381 gctttggcct aaatctatta attgctatct ttgcagtcca tttttgaggt atttctcatt
    34441 ttgtgacaaa aacaattaaa agtctgaagt aagatgtcag gaattattgg aaaaaaaatc
    34501 ggaatgacta gcatttttga cgctgaagga aggaatgtgc cctgcactct cattcaggct
    34561 ggaccttgta ttgttacaca aattagaaac aaagaaactg acggatacaa tgcagttcaa
    34621 ctggcctatc aagatgttaa agataagcat acaacaaagg ctttaaaggg acattttgct
    34681 aaagcaaata caacgcccaa aagaaaagta gtggagttta gagacttccg aatagaattt
    34741 gaaaccgaag tcaaggttgg gcttgaagtt aaagttggtg aagtttttaa agaaggtgaa
    34801 ttcctggatg ccatcggaac ctccaaaggg aaaggatttc aaggcgttgt aaagagatat
    34861 aatttcggag gtgttggcga agctacacac ggtcagcaca atagactcag ggcaccgggt
    34921 tcaattggtg gctcctcctt cccatcaaga gtatttaaag gaatgagaat ggcgggaaga
    34981 atgggaggaa atcgtgtgaa ggcgataaac ttgaaagtaa tgaaaattat tcctgagcaa
    35041 aatttaattc tcattagtgg ttcggttccg ggtgcaatta attcaactat aattttagaa
    35101 aaataaaaaa atgaatcttt cggtatatac tcatagcgga agcgatacgg gcagaaagat
    35161 tagcctggtt aaagagattt ttggtgccga gccaaatgac cacgctatct atctggatgt
    35221 gaagcagttt cttgccaatc aaaggcaggg cacccacaaa tccaaacagc gaaacgaagt
    35281 gagcgggtca acgcggaagt taagaaaaca gaagggaagc ggtgcggcaa gggttggcag
    35341 tattaaatct ccgttgttca gaggaggggg aagagttttc ggaccacaac cgcgagacta
    35401 tagctttaag cttaataaga agctaaaaaa attagccagg atttcagcgc tttcatataa
    35461 agcaaaagat aaagcaatta ccgttgttga ggattttacc tttgatgaag ttaaaacccg
    35521 gaattatgtc aacatgttga attcgctttc ccttggagat aagaaaactc ttcttgtcat
    35581 gcccaaatca gacaagaatg taattctggc cggtagaaat attaaaaagg ccgcaataac
    35641 aacagctgat aacctgaata cttacgatgt tctcaacgcg gataacctat tggtgatcga
    35701 aagttcattg gaccggataa aagaattatt aaaataatga gtgtattaat taaaccattg
    35761 gtcaccgaga aaatgtcagc tcttaatgag aaaggacaat atggatttgt tgtaaacaaa
    35821 aaagcaaata agatccaaat aaggcaggaa attgaaaaga cctatggtgt gaaagtgcag
    35881 agtgtacgaa ctatgagata tggtggaaaa gaaaagtcac gctatacaaa ggcaactgta
    35941 atcgttggaa aaactccatc aataaaaaaa gctatcgtcc aggtagcaga tggggaggta
    36001 atagattttt atggaggtat ttaaaagaat cagagaatgg ctgttaagaa attgaaacca
    36061 acaactcccg gacaaagatt tcgagttgct tctacaagca aggaactcac aaataataat
    36121 ccggaaaaat ctctgaccag atcttccagg aagacaggag gaagaaacaa ccagggacgg
    36181 atgacaatgc gatatatagg tggaggacac aagaagagat tccgtgaaat cgattttaag
    36241 agaaacaagg ttggaattcc gggtatggtt aaaacaattg aatacgatcc aaatcgttct
    36301 gctcgaatcg ctttaatatt ttatccggat ggagagaaaa gatatatcct tgcccctgca
    36361 gggttaaaaa ttggtcaaca aattatttcc ggaccagagg tcgctcccga attaggcaat
    36421 gcacttccgc tgttaaaaat tcctcttggt accattgttc acaatgtaga acttaagccg
    36481 ggtaaaggtg ggtcgatggc aaggtcagca ggaagttatg cccaacttct tgcaagggaa
    36541 gataaatatg caacgctgaa attaccttcc ggtgaaatga gaatggctct tgttaattgt
    36601 tatgctacag tcggttcagt gtcgaattcg gatcatatga atgaatcgct gggaaaggcg
    36661 ggcagaaata ggtggctggg cagaagacca agagtgcggg gcgttgcaat gaaccccgtc
    36721 gatcatccaa tgggtggcgg agaaggaaaa gcatcaggtg ggcatccccg gtcgagaaat
    36781 ggaaaatatg cgaaaggtct taaaaccaga aatagaaata aatattctaa taaactaata
    36841 atagggaaga agaaataatg tcaagatcgc ttaagaaagg tccttatata gacttcagac
    36901 tccaaaagaa ggtggattca atgaatgaat ctggaaagaa atctgtcatc aaaacctggt
    36961 caagaagatc catgatttca ccggactttg taggccatac tttcgcggtg cacaatggca
    37021 ataaattcat accggtgtat atcaccgaaa atatggtagg acataagttg ggcgaatttg
    37081 ccccaaccag aacctaccgg ggtcatgcca aaaaaacagc gtaatggagg cagtagcaaa
    37141 attaaataac gtacccacct cacctcgtaa gatgaggctt gtagcagacc ttattcgagg
    37201 tcagaaggta agcaatgcct tgaatattct gaagtttgaa agaaagaatg gagcgcccag
    37261 gctggagaaa ttgctcttat ctgcaatctc gaactggcaa aataaatttg aagatgttaa
    37321 actggaggag gcggaacttt ttgttaagga aatcagggtc gatggcggaa ggatgctcaa
    37381 gagattaaga ccagcacccc agggaagggc ccacagaatc aggaaaagat ctaaccatgt
    37441 cacgcttgtg atcgatagct taaacaagct tgaagtgaaa gagaaagtgg aaaagacaca
    37501 gactgaggag aagcaaaaac cggtaaaaaa ggaaaagaag aaagccaaga ctgtgaaagc
    37561 aaaaacaggg aaagcaacaa cgaagacaaa aaagattgtt aagaaatcaa ctaagaaaac
    37621 agagaagaaa accaaaggca agaaataatg ggacaaaaag ttaatccggt aggcttaaga
    37681 ttaggaatta ttaaaggttg ggattccaat tggtacgggg gaggtacatt ctctgataag
    37741 ctggttgaag atcagaaaat aagaaagtat gtaaatgcaa ggatacctaa aggaggtatt
    37801 gccaaaattg ttatagaaag aaccctgaag agaataactc ttacaattca caccgccaga
    37861 ccaggtgtag tcattggaaa aggaggaagc gaagttgata agattaagga agagttaaag
    37921 aaaataacca ggaaagatgt tcagataaat atttttgaaa taaaaagacc agaacttgat
    37981 gcaaagttgg ttggggaatc aatcgcccag caattggaag caaggatttc atatcgaaga
    38041 gcgatgaaac aagcaattgc ttcatcaatg cgagtgggag ctcagggtat caaaataaaa
    38101 gtatccggaa gattgggtgg agctgaaatg gcccgaactg aacagtataa ggaaggtaga
    38161 attccgcttc atacattacg tgcagatatc gactatgcaa tttctgaggc acaaacagtg
    38221 tacgggaaaa ttggcattaa agtttgggta tttaaaggtg aagtatacgg taagagagat
    38281 ttatctccca acgtgggcgt atccagttca acctcaggcg gcgcgcaatc agcaggttca
    38341 cgaagaaaag aatccggtgg aagaagaaaa agacgataat tagaaaagag aaatcatgct
    38401 ccaaccgaaa agaacaaaat tcagaagaat gcaaaaaggc cgggtgcgag gtatcgcgca
    38461 aagaggacat acaatcgcct ttggatcatt tgcattaaaa tccatggaac cggcctggat
    38521 tacaagcagg caacttgaag ctgcaagggt ggcagtaacc agggcaatga aacggcaagg
    38581 ccaggtttgg attcggattt ttcctgataa gccaattaca aaaaaacctg ctgaggtccg
    38641 tatgggtaaa ggaaaaggag ccccggaata ttgggtagca gttgtaaagc ctggaacaat
    38701 tctttttgaa tcagcaggag tgccaatcgc tttggctaaa gagtcaatgg ccctggcagc
    38761 acaaaaatta ccaataagaa caaaatttgt tgttcgtaaa gattataccg agagcaaata
    38821 atgaaaccag cagaaattaa aaaactttca tcagaagaac tgagagagaa aataaaaggc
    38881 gaaaaggaat cgcttcagaa aatgaaattt tctcatgcaa taagccctat tgaaaaccct
    38941 atgaaaattc gtgaggcaaa gaaacttata gcaagactta aaactgaatt aacagcaaaa
    39001 ggggatgcca actagtatgc aagcaacaag aaatttacga aaggaaagaa ttggtgaagt
    39061 aattagcaac aagatggata agtccattac agttgcggtg agaagaaaag tgaagcatcc
    39121 tatttatggc aaattcatcc gaaagaccac caagtttatg gctcatgatg agaaaaatga
    39181 atgtggcatc ggtgatactg taaaaataat ggaaaccaga cccctgagca aaagtaaaag
    39241 gtggcggtta atagagataa ttgaaaaggc aaaataaaaa tgatacagca agaaacaaag
    39301 ctgattgtag cggataacag cggagcaaag gaagtgcttt gtattcgtgt gcttggcgga
    39361 acccgaaaga ggtacgccag cctgggtgac aaaatcgtag tcaccgttaa atccgcatta
    39421 acctcaagcg cgttgaaaaa gggaacagtt tccaaggccg tagttgtacg aaccaaaaaa
    39481 gaagtccgca gaaaggatgg atcgtatatc agatttgaag ataacgctgc tgtcttgctt
    39541 aacccgcagg atgagcctcg gggaacaagg atattcggcc ctgttgcaag agagttgcga
    39601 gagaaacagt tcatgaaaat tgtatcactg gcaccagagg taatttaaaa atggaaagga
    39661 gatataacaa acagcccaaa cttcatatca ttaaaggtga taccgtaatg attatatccg
    39721 gaaatgacaa ggggaaaaaa ggcaaagttc tggaagttct tgtttcaaaa aacagggcta
    39781 ttgttgaagg gatgaatatt gtgagcaaac acgtaaaacc ctcagcaaag aatcctgatg
    39841 gaggcatccg gaagactgaa gctggtatcc atttgagcaa ccttatggtg atagaacctt
    39901 cctccggtga accaaccaga accggaagaa aaaagaacga taaaggtaaa cttcaaagat
    39961 attcaaagaa aacaggagaa tttattacca atggctaatc caagactaaa agaattatac
    40021 aaaaaggaaa tcgtcccgtc tctgaaggaa aaattccaat acaaatcttt aatggaagtt
    40081 ccaaagattc aaaagatttc cattaacaag ggtatcgggg aagctgtatc cgacaagaaa
    40141 ctggttgacg taggcgttga agaacttact gcaatctcag gacaaagggc ggtagctact
    40201 ttatcaaaga aagcaatctc aaacttcaaa cttcgtgaag aaatgcccat cggtgccaga
    40261 gttacccttc gtggagataa aatgtatgag tttcttgaca gactcatgaa tgttgctctt
    40321 cccagagtgc gcgacttcag gggagttaat gacaaagggt ttgatggaag ggggaactat
    40381 acacttggtg ttaaggaaca aataattttt cctgaaatca gtattgataa agtcaataag
    40441 ataaacggaa tgaatattac aatcgtgaca acggcaaaaa ccgacaaaga aagttatgaa
    40501 ttgcttaaag cattcgggat gcctttcact aacagaaaca attaacaaat ggccaaagaa
    40561 tcagtaaaag ctagagagag aaagagaaga gtccttgtca tcaggtatgc ggcaaaaagg
    40621 aaggctctga aagcggcagg cgattatcag ggcttggata agttgccgag aaatgcatcc
    40681 ccagtgcgct tgaaaaaccg gtgtaagtta accggcaggc ccaaaggcta tatgagaaaa
    40741 ttcggcattt caagggtaac ttttagagag atggcaagca caggaatgat acctggaata
    40801 acgaaggcca gctggtaaaa gaatgccatt cggctttaaa ttgacagtaa aatgaaataa
    40861 ctttgcaggc ccaaaatggg tgtgcatttt tgctttataa atgaaatgag atgacagatc
    40921 ctattgcaga tttcttaacc cggattagaa acgctgttaa ggcgaataag aggattgtgg
    40981 atattcctgc ttcaaacata aagaaggaaa taaccaaagt actctttgaa aaaggctata
    41041 taaagaatta taagttcgag gatggagtta actatcaggg aaatataaaa atcgctctga
    41101 agtataaccc cgtcaccaag acatcggcgt tggttcaact gaaaagggta agtaagccag
    41161 ggcttaggaa atattcaaag ggtgctgatg ctcccagagt gctaaacggc ttgggtattg
    41221 caatcttgtc tacctcacag ggggttatga cggataaaga ggcacgacaa aagaatattg
    41281 gcggtgagat attgtgtcat gtttattaat caggaataag aatgtcgcga attggaaata
    41341 aaataattga gattcccaaa gatgttacca ttgaggttaa agaaggtaac atttcggtta
    41401 aaggaccaaa aggctcacta tccctggaga tggattctga ctttaaacta aatcaggaag
    41461 aaggctctgc aactttgaaa cggccaactg agcagaaacg acataaggct ttacatggtc
    41521 tctacagatc actgattaat aacatggttg tcggagtaac aaacggatat aaaaaagagc
    41581 tcgaggtgat tggagtggga tataaagcag cagttcaggg gaaagtactt gaacttagtc
    41641 tcggttattc tcataatata tactttggaa ttcctgaaga cctgaaaatc tcagccgaga
    41701 cggtgaaggg taagaatcct actattacta tagaaggcat tgataagcaa ttagtgggtc
    41761 aggtagctgc gaagattaaa tctttaagga aagtagagcc atataaaggc aagggcattc
    41821 gatttgttgg tgaatatgtt agaagaaaag caggtaaaac tgcagcaaaa taagtgagca
    41881 atggcagtaa ctaagctagt aagacgcaga aaaatacgct acggtatcag gaacaaagta
    41941 agtggcacag cggagcgacc aaggttatct gtatttaaaa gtaacagagc tatctatggt
    42001 cagttaatag atgatgccaa agctgagaca atagtagcct ttaactcatc gaaacttgat
    42061 ctgaaaagca acaatacagt tgcatccagc aaggatgtgg ggaaaaagct tgctgaacta
    42121 gcgattgaca aaggaataaa aaaagttgtt tttgatcggg gaggatataa ataccacggg
    42181 caaataaagg cattagctga gggtgcccgt gaaggaggac tagaatttta aattataaaa
    42241 ccatgttgca acaaaatatc agaacagtaa aagcaagcga aatagatctc aaagaaaggg
    42301 ttgttgctat taaaagagta gccaaggtgg ttaagggagg tcgtcgtttc agtttttctg
    42361 caattgtggt tgtcggtgat ggcaatggtg ttgtaggata tggactcggg aaagctaacg
    42421 aagtaacaga tgcaatcaca aaaggaattg atgatgcaaa aaagaaccta gtaaaagtac
    42481 ctattcataa gcatacagtt ccccatgagg caataggtaa gtttggtgga ggacgggtat
    42541 tgcttagacc agcagccccc ggaacaggtg taattgcagg gggtgctatg cgagcagttt
    42601 tggaaagtgc cggagtaaga gacgtgttag gaaaatcaaa aggttcttca aaccctcata
    42661 acgtggttaa agcaactttt gacgcattaa ccagaatgag agatgcatat accgtagcac
    42721 agcaccgcgg agttcaattg agtaaggtat ttaatggata acaagagatg gccaaagtaa
    42781 gaatatcaca agtaagaagc actattaaaa gaccggagcg tcagaaaaga accatcaaag
    42841 ctcttgggtt aggaaagatt aatagaatgg tagaagttga gttaactccc cagattcagg
    42901 gaatgataaa taaggtaagc catttggtat ctgtaacaga attataaaat gaaattatca
    42961 acattaaagc cagctaaagg ctcggtcaga aaaagtaaac gagttggcag aggtcagggt
    43021 tcaggtagag gaggaacgtc tactcgaggg cataagggtg ctcaatcacg ttcaggatat
    43081 tcaagcaaag ctggatttga aggaggacag atgccacttc aaaggcgagt tccaaagttt
    43141 ggatttaaga ataataataa aattgagttc aaggcgatca atctggatac gcttgagaat
    43201 cttgcgtctc aaaccaaagt agcttccatt gacaccggat tattgataaa aaatggactt
    43261 gctgcaaaaa aagataaagt gaagattctt ggacggggtg agttgaaaac gaaaattgaa
    43321 gtaaaagcac atgccttttc taattcagct atagaggcga tcgaaaaagc tggaggaaag
    43381 gccataacta tagaatgaag aatttcttta caaccataaa gaacattttt tccattgagg
    43441 aattgaggac acggatcatc aataccattg ggtatttatt gattttcaga cttggttctt
    43501 acatagtttt gcctggaatt gatccagtca ttctaaaatc aatcaccggc gatcctaccg
    43561 gtatttttgg attattaaac accttccttg gaggcgcttt tcagcgcgca tccatttttg
    43621 cgttgggaat aatgccctat atatccgctt caattgttgt ccagttgtta acggtcgcag
    43681 ttccttattt ccaaaaactt caaaaagaag gagaatcagg aagaaaacga atcaaccaga
    43741 taacaagagt tctgaccatt gctataacat ttatgcaggc tttcagttac ttgtcagtaa
    43801 caattccggc tgaagcaatc actattagcc cattcttgtt caaagcatca tccataatta
    43861 tccttgttac cggaaccatg tttgccatgt ggcttggtga gagaataacc gataggggaa
    43921 taggtaacgg tatttcaatg ttgataatga ttggtatcat ttcaagattg ccatcccaat
    43981 tggtggccga aggcctttca aaggggatgc caggggcttt aatgtttatt cttgaattag
    44041 taaccctgtt ttttgtcgtt atggccgtag taatgtttac ccaggcaatt aggagaatcc
    44101 cggttcaata tgccaaacaa gtggtaggaa acaaagtgta tggggggcaa agacaatata
    44161 taccattgaa agttaattcg gcaggagtaa tgcccataat atttgctcag gcgttaatgt
    44221 tcataccgcc tttgatagca ggcttatggg cagacaacga tatttccatt tacataggca
    44281 ctgtcttcaa tgacacaact tcctggcaat atacgcttgt gtttggaatc ctgattttag
    44341 tatttacatt tttctatacc gcaatcactg ttaatccaaa ccagattgca gacgatatga
    44401 aacgaaatgg agggtttatt cccggagtaa aaccagggaa gcaaacttcc gaatttattg
    44461 ataatatatt aacaaggata acatttcctg gatccgtttt tcttgccata gtagctataa
    44521 tgcccacctt tgctgtgctt gccggagtaa ctcccggatt ttcacagttt tatggtggaa
    44581 catcgctttt gattatggtt ggggttatac ttgataccct ggcgcagatt gaaagttatt
    44641 tactaatgag gcattatgaa ggaatgatga agtcaggaag gataaaagga cgttctcaaa
    44701 atgttgctgt tgcttaatta gtaatgattc attacaaaac agcggaagaa gttgaactga
    44761 taaaggaaag tgctgaaatt cttagcagag ctcatggtga agttgcttcc atgattagac
    44821 ctggcgtgaa gaccatcgac cttgaccata gagctgagtc ttttattaaa gatcagggtg
    44881 gtaaaccttc atttaagaat tacaaatcct tcccattttc attatgcatt agtttaaatg
    44941 aaaatgtggt tcacggcttt cccgggaaat atgaacttca agagggagac attgtttcca
    45001 ttgattgtgg cgtcttttat aaaggatttc atagtgattg tgcatacacc tatccggtag
    45061 gtccagtttc tccggataag gaagaattat taaaaattac tgaagaatct ctttatgagg
    45121 gcatacggaa tgcaaaagca ggaaaccgca ttggtgacat tagtttctca attcaggaat
    45181 atacagagaa acgaggattt tcagtagtgc gcgaattggt tggtcatggt attggaaaaa
    45241 acctgcatga atctcctgaa gtacctaatt atggtaaaag aggcaatggg cctaaaataa
    45301 agcccggatt ggtaattgcc attgaaccca tgattaatat gggccgaaga aatgtggttc
    45361 aggaaaatga tggatggaca ataagaaccc aggacaggga accatctgct cactttgagc
    45421 atacagtagc tatttttgag ggtaaaacag aaatattgac gacacataaa tatatagaag
    45481 agaattttgt aatgaatggc taaacaagca tcgatagagc aagacggaac aataattgaa
    45541 gcattatcca atgcaatgtt tagagttgaa ctggaaaatg ggcaccaggt aattgcccat
    45601 atctccggta aaatgagaat gcattatata aaaatattac ctggcgacaa ggtcaaactg
    45661 gaaatgtctc cctatgatct tacaaaagga agaattgttt acagatataa gtagagttag
    45721 tcatgaaagt aaaagcatca gtaaaaaaga gaagcgcaga ttgtaaagta atcaggcgga
    45781 agggaaagct ttacgtgata aacaaaaaga acccaagatt taagcaacgt caaggttaaa
    45841 atattagaat actatggcac gaatttcagg tgtagatatt ccagatagta aaagaggtga
    45901 ggtaggactc acttacattt atggtatcgg aaggaggtcg gctcaaaaaa tattaacaaa
    45961 agcaggagtt gactggaata agaaagtgag cgagtggaat gatgaagaat caaacaagat
    46021 tcgatcaatc attacggata atttccgcgt cgaaggttcc ttaaaatccg aagtgcagct
    46081 aagtctcaag cgattgttag acattggctg ttacagagga ctccggcatc gtaaaggact
    46141 tcctgttcgt ggacagaaga ctaagaataa tgcaaggact agaaaaggca agagaaaaac
    46201 agtggcaaat aagaaaaggg cacctaaggg ttaatagtta ataaagcgat ggctgaaaaa
    46261 agaaaagaca aagcaagaaa gagagtagtt catgttgaag ctgtgggtca ggttcatata
    46321 caggcaactt tcaacaatat tattatttcc attaccaata tgcagggcca ggtagtttcc
    46381 tgggcatcag caggtaagat gggatttaag ggttccaaaa agaacacgcc atacgcggct
    46441 caggtagcag cctctgactg ttctcaaaaa gccttcgacc ttggcttaag aaaagcggaa
    46501 gtatttgtta aaggtcctgg atcaggaagg gaatcagcaa tcagaactat tcaatcaact
    46561 ggaattgaaa ttacaaccat aaaagatgtt acccctcttc cacataacgg ttgcagacct
    46621 ccgaaaagaa gaagagttta aaaagaaata aaatggcaag atatacagga ccaaaagcaa
    46681 aagtagcacg aaggtttaat gatccaatct ttgggccaag cagggcagtt cagaagaaag
    46741 cataccctcc cggccagcat ggaagaaaca ggagaaggaa acagtcggaa tactcgctgc
    46801 aattgatgga aaaacaaaaa gcgaaataca catacggcgt tcttgaacgt cagttcgaaa
    46861 acctttttca taccgcggct gcaaaaccag gtattaccgg agaggttttg cttcaattac
    46921 tggaagccag gctggataat accgtttaca gacttgggat tgcacccacc aggagagctg
    46981 ccaggcaact agttttacac aagcacataa ccgtaaacgg caaagtcgta aacatagcct
    47041 cttttgaaat aaggattggt gatgtcgttg cagttagaga aaaatcaaaa tcattgttgg
    47101 caataactga atctcttgct gccaatccaa agaaatttcc ctggcttgag tggaatgcct
    47161 ctgctctcga aggcaaactt attagtatgc caatcaggga agatattcct gagaatattc
    47221 aggaacaatt aatcgttgaa ctttattcta aataataatt atctctgttg aagagaaaaa
    47281 tcaaataact aaaaatatgt caatacttgc atttcaaatg cctgaaaagg tggtaatgga
    47341 aaaatccgat gatttccatg gattatttac ttttaaaccg ctggaaaaag ggtatggtgt
    47401 caccattggt aatgcactaa gaagaatatt attgtcctcg ttggagggct atgcaataac
    47461 aggggtaaaa ataccaggtg ttttacatga attttctact gtggaaggaa tagttgagga
    47521 tgtgtccgaa gtgatcctta atttgaaaat ggtgagattt aagaaaatcg ccgaaagcat
    47581 agacagcaaa ataatagtga gtgttaaaaa gcaaagtgta cttactgccg gtgatcttat
    47641 caggtttaca aactcctttg agatactaaa ccctgaccag gtaatttgta atctggatga
    47701 atctgttaat ttggagcttg aattaacagt tgaaaaggga agaggatatt tgccagcgga
    47761 agagaacaaa cctagcgagc aaatttttgg gtttattccc attgatgcgg tttttacacc
    47821 tatcaaaaat gtgaaattta gtgtggaaaa tactcgtgtt gaacaaaaga ccgattatga
    47881 aatgttgatt ctggatattg aaaccgatgg ttcgatacat ccggaaaacg cactgaaagg
    47941 agcagcaaat atcctgattc agcattttat gctgttctct gaccaaaata tggtgcttga
    48001 accaacagat tccggcgaac cagaggctgt ggatgaggaa atgctccaca tgagaaaatt
    48061 acttaaaacc ccgctgaacg acctggattt atctgtaaga gcttacaact gtttgaaggc
    48121 tgcagatgtg aaaaccttgg gagatcttgt gagactggaa atttctgaca tgatgaaatt
    48181 tagaaatttt ggtaagaaat cacttgccga actcgagcag ttgattgctg aaaagaacct
    48241 cacttttggg atggatctct caaaatataa acttgaagaa gactaagaca aatgagacac
    48301 gggaagaaat tcaatcatct aggcaggact tcctctcaca ggaaatcaat gctttctaat
    48361 atggcttcct cattgattct aaagaaaaga atcaaaacta cagtagccaa ggcaaaagca
    48421 ttgcgcctgt atgtggaacc ccttttgaca aaggcaaaaa gtgatacgac acactcaaga
    48481 cgagttgtat tcagttattt gcagaataaa tactcattac aagaattatt tggcgatata
    48541 gcttcaagga ttgctgaacg tccgggggga tatacacgga ttctgaaaac tggaaatcgt
    48601 ttaggtgata atgctgaaat gtgcatcatt gaacttgttg attacaacga aggtctgctg
    48661 aaaacagaga aatcagctaa atcgaagacc aggagaggtc gatcaaagaa gaagtactca
    48721 gattcggaag atacaatgac cacagctgaa gttgttgagg aaaccactgc cgcagtcgaa
    48781 gtggttgcag aagaagaaag tgtcgaaact aagaaaccag aagcttcatc agaaactcag
    48841 gaaggaacta ctgatgatga atcgaaacag aacccggaag cagaagggtc tgatgaaaag
    48901 aataaggaga attaatgcgt aaattgcagt taaatagaga aagggattag agatttatct
    48961 aatccctttt tatttttggg gccaaatcct atccaatgaa aatagcatcc aggaaaaaag
    49021 catatctggt tctgcaggat ggtttgattc ttcagggtat ctcaattgga aaatccggaa
    49081 ccaggggtgg agaaatctgc tttaatacag ggatgacagg ataccaggaa atttacaccg
    49141 atccctctta ttttggccag ataatagtta gtacagctgc tcacattggc aattatggag
    49201 caatgagtac tgagcaagag tcatcaggcg ctaaaatcag cggattagtt gtaaatgaat
    49261 tttcccaggt ttattcccgg gaaactgcag acgaaagctt gcaaaattac ctggaaagaa
    49321 atgaaattgt aggaattgcc gacatcgata cacgtaagtt agtaaggcac attcgcagca
    49381 aaggtgcaat gaatgctatc atctcatcag aacttgagcc ggatgccctt aaaaccgaac
    49441 ttaacaaaat tccatctatg gaagggtctg aactttcatc ggtggtgtca accaaagaac
    49501 cctattttgt tggcgaatca aaaaattcat tccgaattgc tgcccttgat cttgggataa
    49561 agaaaagtat tttgactaat ctggttgaaa gaggatgcct tgttcaggta tttccagcca
    49621 aaactagctt tacggatatg gagaaatggg atccggacgg ttatttcata agcaatggtc
    49681 ctggggatcc tgctgctatg gaatacgcgg tagaaacagt taaggaagtg ctcaataaga
    49741 atattccctt gtttggaatc tgccttggcc atcaaattct tgcccgtgca tgcggaataa
    49801 gtacttacaa gatgcaccat ggtcaccggg ggcttaatca cccggtgaag aatttgaaca
    49861 ccggaagaag tgaaatcaca tctcagaatc atgggttctg cgtgaacgga gatgaaacca
    49921 gtaagaataa gaatttaaaa attactcacg ttaaccttaa tgacaaaacc atagaagggt
    49981 ttgaagtatt gaataagaaa gcgttcagtg ttcagtatca tccggaagcc tcgccaggac
    50041 cacacgactc ccgttacctg ttcgacaact tcatcgaaat ggtaaaaaaa tctaaaaaga
    50101 ttaacgtatg agctttattg aagaaataat cgcccgacaa atttttgatt cgagaggtaa
    50161 tcctacaatt gaagttgacg ttctgaccga aaatggattc ataggacgtg ctgcagtccc
    50221 atctggtgct tccacaggaa gccatgaagc ggttgaatta cgagatggag gaccgaatta
    50281 tatgggaaag ggtgtattga aagccgtaaa gaatgttaat gaacttattt cacctgaatt
    50341 gatcggaatg ccagttatgg agcaaacttt gattgataac ttgttgatac aattggatgg
    50401 ctcacccaac aaaagcaaaa tgggtgctaa tgccatttta ggggcctcac tggctgttgc
    50461 aaaagcagct gctcaggaat caggtcaacc tttattcaga tatgtcggtg gcgtaaatgc
    50521 ttgcacaatg ccaattccta tgatgaacat tttaaatggt ggaagccacg cggataactc
    50581 aattgatttt caagagttca tgattatgcc aatgaaggct tcaagttttt ctgaagctct
    50641 gagaatgggt accgaagtgt tccacaatct gaagaaggta ttaacctcca aggggttgtc
    50701 gactaatgtt ggagatgaag gaggatttgc tcctaatata aaatctaatg tagaggccat
    50761 tgaattggtt ttgattagta tcgagaaagc gggttacaaa cccggtgaag acatctttat
    50821 tgcgctggat gctgcttcat ccgaatttta tgatattaaa aagtcaaggt acattctcaa
    50881 aaaatctacc agggaagaac tatcatcatc ggaactggct gcattctgga aggagtgggt
    50941 agataaatac cctattgttt caatcgagga tggtatggcc gaagatgatt gggacgggtg
    51001 gaaagaactc acgaatgtaa ttggcagcaa agttcagatt gtgggtgatg acctttttgt
    51061 gaccaatgtt gaaaggttac aaaaaggaat tgatgagggc attgccaata gtattctgat
    51121 taaagttaac cagatcggaa cgctaacaga aacaattaat gcggtgaatc ttgccacccg
    51181 caactcctat acttcggtaa tgtcccatcg atccggagaa acggaagata ataccatagc
    51241 tgatttggca gtggcactta ataccggtca gattaaaacc ggatcagcct ccaggtcaga
    51301 cagaatggct aaatataatc agcttttgag aattgaagag gagcttgggg aggtggccta
    51361 ttttccaaat aaattgaaat aatcagggaa gtaatagtaa ttccttttta ttttactgtt
    51421 attaatatta tttttaggat aatttgaata tgatatgaga gtcaaattac ctgcctatct
    51481 caagaatttc tactttataa tcgcggtatt ctttttgatc tggatgcttt ttgtcgattc
    51541 aaatgatgtt ttttcccaac ttaaactcag aaaaaaggta actaccctcg aacaagaaaa
    51601 ggaatactac ctggagaaaa ttgaagaagt gaaaaaggat cgggaggagt tgctgagtaa
    51661 ccaggaactt cttgaaaaat ttgcccggga aaaatatctg atgaagaaaa agacagaaga
    51721 tatctatctt gtagttgagg aaaagtagaa ttaatgaaaa agtttttgct ggtacctttt
    51781 atcctggtct cattcccggc tatatgtcaa tatgatcaat tgatcggaaa agatgttcca
    51841 ttaatagaaa ggttttatgt gggtggcggc tttggtctgg gatttagcag tttctcggat
    51901 tatgttgccg tctctccaaa tattggatac cggattacag aaagagggtc aattggtatt
    51961 ggagtattgt atcagtatag aaactataaa gcctatgatg taaagactga tgattggggc
    52021 ggcagtatct ttacaaggta taaagttttt ggcccggctt tcgccgcagc agaatacgaa
    52081 tatataaatt ttgaattcgt aactgcaaca ctaaccacat ttcgtggtga ctattcaagt
    52141 ttcatggtag gtgcaggaat tgctcaacca ataacacgga acgtttcttt catcatttct
    52201 gcgatgtaca accttactta cagggaaact gacggtgggc cgtattcctc accctggaat
    52261 ttacgcgtag gaatatctgc aggtttttaa aaatcaagtc cgaatttttc cgccagtact
    52321 ttaagtgatt cttcaacctt aggatccagg ttaatcccgt tttttagccg tttaatttct
    52381 tcctctcgct ccacatcgcc tggtattaat accctttcct ggccgtcgat ggtttgtgca
    52441 tttctgaacc tgccaatcca tttatccatg ttttccttga attcttttgc gtctctgaat
    52501 gcatcaattc tcattactcc aaaaaaatga cctatgccat caccaaccaa atcgttcctg
    52561 actggcagga agctgacaaa cggcggcacc cacggaccat aatttgcgcc agataaaatt
    52621 gctgaaaaaa tatctaccag ggaacccagg atatagcctt tgtggccgcc tgtttctctg
    52681 attccgccta aaggcaataa tgcgccgcca ttttgaactt cgttaggatc ctgagagggg
    52741 aggccttctt ttgtctgcac ccaacctatt ggagcctttc caccctgtcg ctgcagtatt
    52801 tcgagctttc cattagcggc ggttgtggta gcaaaatcag caacatatgg aggttctgta
    52861 ccggcgggaa tcgccacaga aattggattc gttccaagta gtctttcttt agagaatgtc
    52921 ggagcaacga gtgggcttgc atttgtcatt gagattccaa tcatgtcatg ctgcaaggca
    52981 agcattgaat ggtaggcggc aattccaaaa tgatttgaat ttctgactgc cacccatccc
    53041 gttccgactt tttttgcttt ctttattgca atctccatgg cgaatggtga cacgaccaaa
    53101 ccaagtcctc tatcaccatc aactactgcc gtgcccggtg tttcatgagt tattctgatc
    53161 accggcttac tattgattcg acctgcttcc catagtctaa catatccggg aagccgggca
    53221 gatccatgcg aatctatccc acgaaggtct gctgaaatca aaacgtctgc tgcaaggttt
    53281 gcatcaatgg ggctacatcc cattttaaga aagacgttgg tgcaaaattc tttaaggcgg
    53341 gcaggtataa ccatgtttga aattacacca gatatttttg tgatttcaaa aattctgaaa
    53401 aaattccctc ttttagagaa taagaggaaa cccgaactcc ttcaaatgag ttcatcatta
    53461 taactgattg gattaaaatt gaagcaacca caataagatc tacccgcatt tctatcatcc
    53521 ctgaaatttg cagtctttgg gctctgtttt tagagattat ttcaagctga atatctttca
    53581 tcccttcaat ggacaatgga agttcggttt ggtccggaac aatcgcgatg tcattttttc
    53641 tcctgtaaat ctcactgagg gtgtcaaatg tgccggaaga tcctataagg attttaggtt
    53701 tgtaaacatt gagggcctga tacagaggag taagcttttc aatcaaatat tgttcaacat
    53761 ttctgatttc accgggaaga ataggatcat tttcctgaaa tttgtcgctt aatctttgtg
    53821 cacctatttc aaggcttttc ttccatttta ttccagagga gttcccaata acaaactcaa
    53881 cacttccgcc tcctatgtcc attatcaggc catctgcttc atccattttc aatgctaatg
    53941 aaacaccttc aaagatataa gccgcttcct tttcacccga aatgatctca atatccattc
    54001 ccgttattcc agtcaactgg tcaattattt ctgacgaatt tccggaactt ctgagtgcgg
    54061 aagtagccac acattttgta cgctcaactt tgtacaagtc aaggagattc ttaatatgaa
    54121 gagcggcaga aaatgctctg tttgctgact caggggttat aacacctgta ttgattccac
    54181 cctggccaag agcaacatga attttttctt tatggataaa tgaatactcc atcccctcca
    54241 tttccgctac cagaacatga aaagtattcg ttccaagatc aactattccg actcttgttt
    54301 tcataaaaaa tttgacgcga atatagtgtg aagttgagat tccgtcaatt caagtccttt
    54361 atttaatcat tatggaattg cagggctaaa ttctccaaag tttcttaatt tggctttcta
    54421 cattacaaaa tcatatggca aagaaaacct ccaaatcgaa gtctaaatgg gtaacatcca
    54481 aagagaagtt cgccttgctt gaaaggaaaa acaagcaggc tttgcttggc ggaggtgaag
    54541 acaggattat tgctcaacac aacaagaaca aattaactgc cagggagaga attgaattac
    54601 tgattgataa cggaactttt aatgaaattg gaaaatttgt caaacatcga tcgaaagatt
    54661 ttggacttga taaagtacag gtattgggtg atggggttat taccggctat ggattggtga
    54721 atggaagatc tgtctacgta ttctcgcagg attttacaat tttgggaggt tcgttatcag
    54781 aaactcatgc ggagaagatt gttaaactga tggatcttgc actaaaaaat ggaattccct
    54841 taattgggtt aaatgattca ggaggcgcga gaattcagga aggggtagta tctctggggg
    54901 gctatgctga tattttctac cggaacacgc tggccagcgg cgtgatacca cagatatctg
    54961 cggttatggg cccatgcgcc ggtggtgccg tttatagtcc tgcaatcaca gattttattt
    55021 taatggttga gcacacctct tatatgtttg tgacaggacc aaacgttgta aagtctgtta
    55081 cacaagaaga agtttctgct gaagaattag gtggtacttc tacgcacagc accaaaagtg
    55141 gtgtaactca tttttcctgt gccaatgagg tggaatgtat tcaacaaata aagcagttgc
    55201 ttagctatat gccccaaaac tgtgaagaac aacctcccct gtttgaatat gacccttctg
    55261 aagaattacg gcctgatttg gacacaataa ttcctgacaa tccaaatcaa ccatatgata
    55321 tccgtgaagt aattgatcaa atcattgatg gaaatacatt cctggaagtg cacgaaaatt
    55381 ttgctgaaaa tatagtagtg gggtttgcca gactggctgg tcgaagtatt ggcattgtgg
    55441 gaaatcagcc ttcagtactt gcgggtgtat tggatatcaa ctccagcatc aaaggcgcaa
    55501 ggtttgtgag attttgtgac agcttcaata ttcccttgct ggtattggtt gacgtaccag
    55561 ggtttttacc aggaactgac caggagtgga atggtattat tactaatggc tcaaaattgc
    55621 tttacgcatt cagtgaagct accgttccac gtataactgt cattgtcagg aaggcatacg
    55681 gcggagcata cgatgtgatg aattcaaagc atattggagc cgaccttaat tatgcctggc
    55741 caagtgcaga gattgccgtt atgggtgcca aaggtgcagc cgagattatc tttagaaagg
    55801 aaattcaatc ttctaaagat cctcagaaaa agctggacga gaaaatagat gaatacacca
    55861 aaaaatttgc tcatccttat attgccgccg ccaggggatt tattgatgaa gtaatttacc
    55921 ccagggaaac aagagaaaag ttaattggag ctttcaaaat gctggaaaat aaggtggata
    55981 agattccaag taaaaagcac ggtaatatcc ccttgtagtt ttatgaacga gaaagttatt
    56041 atagtaacag cagccagcaa aggaatcggc cgggcttgtg caattaaatt ggctgaacaa
    56101 ggttgtaaac tttcgctgat gtctcgatcg gaggaaattt cggaagttct tccaggaaaa
    56161 gattttcagg caactattgg tgatgtagga aaattggagg accttgaaaa actggtagct
    56221 catacgatgt caaaatttgg cagggtagac ggtgtggtta acaacaccgg acatacagcc
    56281 aagggagatt taatttctat atctgatcag gaatggcagc aggcttttga gattgcattt
    56341 ctcaacacgg taagaatggc acgacttgtg actccgctta ttcagaggca gggtacaggt
    56401 tcaatagtaa atatttcaag ttttgcagct aaagaaccat ccttgctatt tcccacttct
    56461 tcagtaatca gggctgcttt gagcaattat gctaaactgt acgctacccg gtttggacat
    56521 aggaaaatcc ggatgaataa cgtccttcca ggctatgtgg actcgtatcc cctggataaa
    56581 gaaacaataa aaacaatccc aatgggaagg ccggccaaga ccgaggaaat agctaattta
    56641 gtcgcatttt taatatcaga tgaatcatct tatatcaatg gtcaatcagt tgttattgat
    56701 ggcggactaa ataaatcctt ttaattatgg acaagaagag cgaaaagttt ttatatcaat
    56761 atcttaacaa tgcatcgcca accggctttg agtcttccgg tcagcaaatt tggctggatt
    56821 acataaagtc ttatgttaac aaatatttta cagatacata tggtacagca gtgggagtaa
    56881 tcaaccccga tgcaaaatat aaggtggtta ttgaagccca cgcggatgag atttcctggt
    56941 ttgtaaatta cattactaag gaaggttaca tttatgtaat aagaaatgga ggttcagatc
    57001 acattattgc accatccaag agggtaaata ttcacactga taaaggaatc gttaaaggag
    57061 tttttggatg gccggctatt cacgttcggg ataaagattc agaaaaagca cctgcccttg
    57121 ataatatctt catagacgta ggatgccatt caaagaaaga ggtagaacaa cttggagttc
    57181 atgttggatg cgttataacc tttgaagatg aactcacaac ccttaataaa cgataccttg
    57241 tgggccgggc tctggataac aggatcggcg ggttcatgat tgccgaggtt gcaagaaggc
    57301 tgaaggaaaa gaaaaagaaa cttccgtttg ggctgtatgt agttaattcg gttcaggaag
    57361 aaatcggctt gagaggtgcg caaatgattg ctcaaaaaat tagtcccgac cttgccatca
    57421 taacggatgt ttgtcatgac acgcagtcac ccatgtataa taaaataaag agcggagatc
    57481 aaaaatcagg tgatgggccg gttttaactt acggacctgc tgtgcaaaac aacgtgttga
    57541 aattcattat agaggttgca aagaaaaaca agattccatt tcaacgacaa gcggtgtccc
    57601 ggtcaacagg tacggatacc gactcttttg cttattcaaa tgaaggtgta gcatcggcgc
    57661 tgatttctct tcctcttaaa tatatgcaca caacagtgga aaccgtgcat tttgctgaca
    57721 ttgagaacgt aattaagctt atttttgaaa ctgtgttgag tgttaaacca ggtcagaatt
    57781 ttaaatacct aaataaggct acctgataat aagtatcttc tttgatcgaa aaactccgtc
    57841 tcctgttaaa gtaacaatat ataaattgct aatcaaagtt ggaagttcaa ttcggatttc
    57901 gggatcattg aattgcttag tgtaaacctc acggcctgtt tgatcaacta ttttcatggc
    57961 taaattcctt ccattgaaat tttcaaggat taaagaataa atactttcac tttttataat
    58021 atgaaaatca aacttaccgg aattagacag gaataaaaca ttggagaatt tctgtttatt
    58081 tccatcggaa attccaagta tcctgaaata agcctggcct ggaacctcgt caagatgcat
    58141 atagctgtaa gacgatttac cattgacagg ggtaacctct gcaaaggtct gaaactcgct
    58201 cccatttaaa cttttttgaa tttcatacgc aatataaatg ctcgtcggat tagttttcca
    58261 gtctaaggat atctggctgc caagcttatt ggtagaaagt tcaatttcat taacaggaag
    58321 gccggtaata cctgttgcct tcccaaacgt gaaagtgctg aacgaagtta catttgcggt
    58381 tatgccacct tctcctcctg cacctatgct gctgccggga gtaatacttc caccaatatt
    58441 ttgccagggt ccggttccag agtattttga tataattaga tcagatgaca agacatcaat
    58501 gtcactttgg ttggcatctt tccaaaaaaa agtaacatcg cccgtcgccg taccgctcag
    58561 ccggtttaag gtccaatact ccagactgct cacatgatta attgcagcat caagatttgc
    58621 catgtcgaca ggttcctgat aatggtaaga agctgcaaat aagtttgagg aagttcctcc
    58681 ggtcaggata gaaatggaaa caggtgccca gattcccgca tctccggtgg gaaaggtgaa
    58741 gtctcccggg atatttcctc ttttggcaac ggttccttca ataaagctgc cattattgcc
    58801 tgtggaagaa tttgcgccat tttcaattgt aacatcaaat gaattggttt caataatgcc
    58861 attcactaaa gacagaaaat ctaaaatctg tagcctaaac ccaagtgaag ccgtgcctcc
    58921 tgatttgttt atttccaaat ttcgaaaaga agggatattt gaagtcccat caagacgaac
    58981 aaagttttga tttgtggatc catccagaat agttgttccc gataactcgc taaatccgaa
    59041 ggctggactt gaagaatttg tatttatgat aatatctcca gcaattgtac aatcactgtt
    59101 ataacacggc tgcagaaaac ctgtattgga tttaataagc gttaagttac cattaaagtc
    59161 atctggttta cctgggtcgt atgccaatct taggcgagct gttccggcat tctcaaaagt
    59221 tacatttgag ttaaacgtat ttgcacccgg gcagtaacta tttattgtac cggttgtttt
    59281 gaaagaggaa gaattattaa aagaatttcc ttccaggagg aggatgggtg tttccacaat
    59341 taccggagca ttccaaatgg tgttaataaa atttagcgtt gaggaaccgg taagggaaag
    59401 attataggtt gaagcagatc caaattgaga tacatttctg agacgcagag tgcctgtctc
    59461 gaatccggtt gatccaatat tgacaattgc gccagggttt aatgttacac ttcctgcgtt
    59521 gccaagataa acccccgcgc cactggtaca attaacagat atgctattat taaaggtgga
    59581 actggatgag gaaaattccg agaagcgtat atatccccct ccgcccgtac tctcctgatt
    59641 tactataacc gggccattaa aaacattgcc ggaactatag ccgctcatga agatcctgga
    59701 taaggaaccg taattattta aagttacctg accattataa acatctggcg gagtagccgc
    59761 gttagcaccg aaaaggataa agtaattgct tgaattggta atacttagat caccatcata
    59821 ttggttgcct cctaagctgg cgtcatcaag atctccggtc ttctccacag ataaagagtt
    59881 acggaacctg ctaaatgaag taaagaatct tggtgattca atttgaatag gacgattaaa
    59941 tatgcacgat tttatgttaa gcgtagctcc atctgtcagt atcaaatcca ttaagtctgt
    60001 attgaaatcc tgaatgcttt gaagagttaa agtgcctcca gtgaaattgg aaattttgat
    60061 attggctccg ggattgaaat ttatttggga agtggcattt ccgaaatgaa tatttcccac
    60121 tgcgttatca atacttatag tagaactaaa attggaagta ccgctataac ctagccagag
    60181 agtgccggta ccataattat taaggtcaat taaatcttca aaagtatctc ccgacagatt
    60241 tcccatgatc agattgccgg cgccggaaat ttccagcaca gaattagtgt aaaaaatgtt
    60301 gtcacctgcc gaataattat ctgcatttcc cgatttgctg aaataaaccg attcaataaa
    60361 ttcatttcca cttaaataaa catcggaact caatgagtaa gtaataactt ctaagcttgt
    60421 attttcaaat ctggttgttc ctgaattgaa aataattagg aagggagcgc caatggaaga
    60481 aaatattgat cctgaggtaa tttctacttc accgaaaata gaaacatcat tgccatcaag
    60541 gtctatggtt cctgtgaagt ctgttatata caatcctgcc agtgaaatgg atgctgacaa
    60601 cttcgcgtca taatttgaac tggaggcgga aaagtaaact acatccgttg ataatggtgt
    60661 ccccggggga gtccaattta ccggattatt ccaatctcca tccaatgatg aatttatcca
    60721 ggttttagtt tgggcattag agtactggca ggcgcaaaac agatcaaaaa tcagtaaaaa
    60781 ggaaaatata ctattccaac aattcatagt ttaaatatat attaattaat acatgaatag
    60841 tataaaaaga taggtaaaaa attacacctg ttatttaatt tgcaaattcc ataacagatt
    60901 caagctgatt ccaaaaacct ggatatgatt tgttaataac tgagggattc tcaatcaaaa
    60961 catcagtgaa gagcataaga ggggcaaatg ccattgccat tcgatgatct tcataagtgc
    61021 ggattcggat ttgctttggt agttcaccag aaatagttaa aaagcttaaa gaatatgaat
    61081 tatcctgatt ttttaaaaat gccacaccaa tttttgaaag ttctttttgt atggctttga
    61141 ttctatcggt ttctttaatg gagagggtat ccaatccatt gaactttgcc cttactttaa
    61201 gtaaggcaca caaaactata atagtttgag ccaggtcagg attttcaata aaatcgaaga
    61261 tctttctctg tctaaattta atcctggtca actcatatcc acccggaata ggtcgagatc
    61321 gtactccgaa acccttcata attgagatta attttgcatc accctgccgt gggtgatggc
    61381 taagaccagt caaaagaatc cttttagcgt tggaaagcct gaacaatgaa taccaatagc
    61441 ttgccgctga ccaatctcca tctactttta ctttacgctt ccggtatttt ccctctttga
    61501 taaaaatgct acttgccttc acaattggct ttattccgaa ttgccccatc agcgaagaag
    61561 taagcagaat atagggtttg ctaacaacct ttcctgctaa agttatagtt agaccgccgt
    61621 ccaattttgg accaatcatc attaatgccg agataaactg actgcttatg ccaccgctta
    61681 tttgaacttt atttttcccc gagtgcacaa aatcagcaat ttctattgga ggaaaccctt
    61741 tgctgcccag atacttaatg ttagcaccta acttcttaaa tgcttccacc aggtccccaa
    61801 ttggcctctt atgcattctt tctgaacctt tcaatatttt acgtttgccg gtaacagcaa
    61861 agtaagcagt cagaaatctc atggccgttc cggcatctcc aacgtcaatt gttttgcctg
    61921 agttggtcaa agctctttgg aaaataagtg tatcccgcga ggaagataaa ttcaggaggg
    61981 ttgaaatact acccgacaat gcattaatca ttaaataccg gttgctttca cttttggagg
    62041 gcggaagagt aatttttgct ccttctttta attgggattt ttttaagagc aaggattcac
    62101 ttttcatgat taagtaaagt cagaaaggtt tagtagaatt ttttcgtgca aatttggcaa
    62161 cttttttgca tatttaggtg ttaaatataa ataacaaatc aaagttaatt agaaagtaaa
    62221 tgggttcaac gggtaaagct tttgcgttat tagtaggatt ggcagcaggt gctgcgctta
    62281 cggtattcgc tttttcgaaa aaagggaaga aaactcgtga agagatcgca aggaagttta
    62341 atgaaattgc tgatggagta aaaacttcag tcaggaaaag cctgacaaag gtatcttagg
    62401 ctttaaccag atgattgtaa aaagaaaaac ttttttcaat ttcttcaacg gaaacttcaa
    62461 cattcacctt cacctttcca attccggata ttagggtcat tagtatccgg ccttcaatat
    62521 tttttttatc gttttttaaa taattcataa tttcaggctg gctctcaggt gagatttttc
    62581 tgcaaccgaa attatgaacg attaatttgg agatatcctg caaagccgga tccggtaagc
    62641 ccagtttgag cgcagataca aagctttcac acaaaaggcc tgcggcaact gcctctccgt
    62701 gaagaagttg gttatttttt tccatataat aactttcgag tgcatgccca attgtatgcc
    62761 cgaaattaag cgattttctg acttcttctt caaagggatc ctgaacaaca aagctgtgct
    62821 taatttcaat actcctttgg atgaccttat cccacttgat ttcgtcaaag gaggtggtaa
    62881 gtaaatattg aaagtatcca ttatccgcga taaggctgtg cttaataatt tccgcaaagc
    62941 cagactttac atgtctttta tcctgcgttg ataaaaaaac aggatcaata atcactgcaa
    63001 aaggttcact gaaaacaccc agttgatttt taagggacaa gaaatcaatg ccggtttttc
    63061 caccaatact ggcatccacc tgtgccagca atgttgtagg aatattaata aattctattc
    63121 cccttttaaa agttgcggcg caaaatccac ccatatcgca aattacaccc ccacccaaat
    63181 tgattagcag ggaagagcgg tcaaagcctg cgttagtcat tgatgtccat atgtgattgc
    63241 aggtttccac gttcttataa acctcccctg acttaatttc gatgattccg caatggctta
    63301 cggcagaaga aatcaaagga aggcaatgct ttttagtatt ttcatccacc agaattccgg
    63361 ttcttgaaaa atgattcttt gaaaaaaacc cttcaagatc atttgaaacg ctggaagtaa
    63421 acatcagatt ttcaggtagc atttctactt cgactctttt ttggaattga ttgcggtctg
    63481 aatcctgatg gattcctcgt gaataacttt atacaactcc tcaataaatc ttgggcttaa
    63541 ccccaaaact tttgcccact caggcctgga ttttagaatt tccttccaac gttcaagttg
    63601 aaagacggcc acattatttt cttttttgta gtcacctatt tgctcaacta atttcattct
    63661 tgttgaaaga gcttctatga ggtcccggtc tgctatattt atcttttccc ttaactcttc
    63721 gagaagatca agaaacagtg gatctgtaaa gttttcagaa cgtatttcaa gtctgttcaa
    63781 tagttcacca agacttgtgg gcgttatttg ttgttccgaa tcgctaagcg ccgtttccgg
    63841 gtcagggtgc acttctacca tcaagccatc ataattaaga tccatacttt tttgagcaat
    63901 ttctaaaatc atacttctct ttcccgctat atgactggga tcgcatatga gaggcaaatc
    63961 cggccttatt cttcgcaatt caagaggtat ttgccaaagt ggaatgttcc tgaatctgct
    64021 gggttgaaaa cttgaaaaac ccctgtgaac cgcggcaacg tctttggttt tcttctctat
    64081 tctctctatg gatcccagcc ataaacctaa gtcgggattg ataggatttt ttatcaggac
    64141 aggcctttga attcctgata aggcctcggc gatttcctga accacaaaag ggtttactga
    64201 agtccgggcc ccaatccaaa acacatcgat ttcatatttt aaagcctgtt caacatgata
    64261 aggatttgcg atttccgttg tcaccttaat ctcaaattcg ttgcgaattt tgcccagcca
    64321 ttcaagcgcc agaactccaa ggccttcaaa tgtatccggt cttgagcggg gtttccatga
    64381 acctgctctt aacaaaggaa tgttcatttc ctttaacgct cctgccacaa gcgctaattg
    64441 ctcatagctt tctacgctgc atggacctgc gataataaaa ggtttttctt tcctgaaaaa
    64501 tgattccaaa atgatgaatg ttaactaaaa actaagttag ttttgggaga tcgtacctcc
    64561 taattaataa gtagattctt ttatatttgc gcgattcaaa acccgggttg atggacttaa
    64621 aaacttatgt agatttcgat aacaccgagg ttgctttttc ttcaaaatca aaccgaaaac
    64681 tttttaaagc caggcttttg ttttgggttg taaatcatcc ctttctggca gcaatttcaa
    64741 caggcttggt cagagtcgtt atttttttcg gactgcctgt aaattggatt attaagccta
    64801 ccgttttcga acatttctgt ggtggtgaaa ccattaaaga gagtgaaggg tcaattgcag
    64861 aattggcatc attcaatatt aagacaatcc tggattactc tgtggaaggc gaggactctg
    64921 aagaggattt tgaaacaacc aaaagagaga ctttaagaac cattgaaaag gctgcccaaa
    64981 attcaaacat ggctttttgt gttttcaaac ccaccggttt agccagtcag ataattatgg
    65041 aaaaagttca gctcaggaaa aatctaactg atgaagaagg aagaaactgg caaaaagccg
    65101 ccgaaagatt tgatgcaatt tgcaatgcag cgtctgaaat gaagattcca atattaattg
    65161 atgctgaaga cagctggatt caggatccca ttgataagct agccatggat ttgatgagaa
    65221 agtataacag ggagactgct attgtattca acacttatca actttataag aagaatgcgt
    65281 tgcaggatct aaaaagagat tgtgaaagag ccagaactga aaaatttttt ctgggtgcga
    65341 agctggttcg cggtgcttac atggaaaaag aaagagaaag agctgaagaa aaagggtatc
    65401 ctgatccaat aaacgaatca aaagaagata cggacaggga ttataatgct gcgttagaat
    65461 tttgcattga aaatattggg caattatact tgttttcagg ttctcataat gaatactcaa
    65521 attattacct gaccctttta atggaaaaac aaggattggc gaagaatgac aaacggattt
    65581 ttttcggcca attgtatggt atgggggatc atatttcttt caatttagcg gccgcaggtt
    65641 ataatgtggc aaaatatgtc ccctttgggc ctgtcaagtc agttatgccc tatcttttcc
    65701 gtagagcgga ggaaaatact tccgtgaaag gtcagagttc ccgtgaactt accttaatcc
    65761 gtaaagaaac acttcgacgg aagtccggat agggcatttt ggggcaaaag taataacttt
    65821 gcagtcaatt tttaagcatg gaattaagcg aacaggaatt aatcagacga caggctaaag
    65881 ctgaattgga aaagttggga ataaatcctt acccacctga ggaatatccg gtcaccacca
    65941 cttccggaga ggtaaccagc ggatttgagg cgaatcccga agcctttaaa gatctaagta
    66001 tagccggccg aatcatgagt cggcgaatta tggggtcggc gtcttttttt gagattcagg
    66061 attcatccgg tagaattcag gcttatgtta gaagagatga tatatgccca aatgatgata
    66121 aaacttttta taacacggta ttcaaaaaac tcctggacat tggagacatt gttggaattc
    66181 atggatttgc tttcaaaacg caaaccggtg aattaacgat tcacgtaagt gaactcaaga
    66241 ttctcagcaa gtctttgcgc ccgctgccaa ttgttaaaga aacggtagag gttgatggaa
    66301 gcataaaagt gcacgacgca tttaaagatc cggaggcaag atacaggcag cgatatgttg
    66361 atttaatcgt aaatccgcaa gttcgggaag tattcaggaa gcgaacccgg cttatgaatt
    66421 ccatgcggag gtttcttgat gaaaaaggat atcttgaagt tgaaactccc gtcctccagc
    66481 ccttatatgg cggggcagct gcccgaccct tcaagactca tcacaatgcc ctcgattcaa
    66541 ctttgtattt gagaattgcc aatgaattgt atctaaaacg cctgattgtc ggcggatatg
    66601 acggcgtcta tgagttctcc aaagattttc gcaatgaagg tatggataga ttccataatc
    66661 ctgaattcac ccagatcgaa ttgtatgttg cctataagga ttacaattgg atgatggaat
    66721 tggtagaagc gatgattgaa aaagttgcct tggatctaca cggcactaca gtggtaacag
    66781 ttggtgataa taaaattgat ttcaggaaac cctggaaaag atactccatg tttgaagcga
    66841 ttcagcattt tacaggcgtc gacatatcta aaatggatga acttgaatta agaaagacag
    66901 ctaaggaaat gggtattgaa gttgaagcca caatgggcaa agggaaaatt attgatgaga
    66961 tttttggtga gaaatgtgaa cccaaactaa tacaacctac ctacataaca gattatccgc
    67021 ttgaaatgtc accgcttgca aaaaagcaca gatcggttga tgggctggta gaaagatttg
    67081 aagcaatcgc taacggaaag gaaatatgca acgcgttttc cgaattgaat gatccgatag
    67141 atcagcgtca acgttttgaa atgcagttag agctgggctt aaggggggat gaggaagcta
    67201 tgatgatgga tgaagactat ataagagcgc tggaatatgg aatgccccca accgcagggt
    67261 taggagttgg tattgaccgt ctaaccatga tgatgaccaa ttctcactct atccaggatg
    67321 ttttattttt cccgcaaatg aaacctgaga aaaaacccgg ggtttccaac gatgccgagc
    67381 tcgaacaact gggaataaat gccggactga ttcccgcaat ccgcaaatta ggcattaata
    67441 ctattcagca aataaaatct tccaatccaa acaaattatt taatgatgtt tgcggaacgc
    67501 ggaagaaaat gaaattagat gttgccaacc ccactttaga ggaagtaaaa aaatggatag
    67561 aaagttgatt acctgtcaaa gaagcttcgc cgtctgttga tttttagatc ctgaagcatg
    67621 gaggatttaa ctctgattgt aaaatcgtaa gattcaaatc tgccaaaagg tgtccatgta
    67681 aaaagcattt cccagcagtg caggtttcgc gttaaagaga attgagtttg agtgaactcc
    67741 ttactttcaa aatcgtatcc cgatgtaaat gttatcatgg tgttttgagt aatccgcaaa
    67801 tcaccattaa atgataatga ttgagtgata ttcggatctg ctaaccctat tttgcttcga
    67861 gtaagactgt aattggcact cagattccat ggcaaatcga aatcaacata cagatttgga
    67921 ttgtttagca taaactcttt ctccgcgtca ccggccttgg atttttctat tttttccttg
    67981 gttgaaaaag tggcgggatt caggcttgag ctcaggccaa tggttaatga acttagttgt
    68041 ccgattccct ttccgtgctc ccaggtatac tcatctaatt ttacctgatt acccgcatcg
    68101 tcttctctgt acacataagg atctattgaa ccattgatag aggcatttaa cttgcctccg
    68161 aaaagacttg ttgcagtgga gatggagata ttacttaatc gataccgctc cgcgaggaaa
    68221 ttatatcctg aactcacgga taaattatta agcaaggtaa ctttttttgt tttttgcaaa
    68281 gaatcacttt tatccagcac tttagcttcc aggttatttc ccaggctgaa ccctatactt
    68341 ccgtttcgtc cggttcctgg tactccataa attgcggact cgaacctgtt agcatatttc
    68401 aggtttccat ttgcatcatc atactgttca taataaccga atttttgctt accgaaatca
    68461 ggccggtagg aaacagaaac cgtgggaatt aatgtatgcc ttatcgctct caaatatcct
    68521 ttcttaaaca acaacgttcc atacaaacgc gtggtcatgg atgcatttgt ggagtaatca
    68581 tatactctgt aaaaaccaga tgaagtgtct gatttgacct tggtttgccc atcgtccaca
    68641 taatcaaatc tttcaaaata ccatctttcc gtgtaattga aactgggggt aagtgtaaag
    68701 aatttaaaca aactcatgct ggtggatatg ggaatggaat ggctcagccc tcttttagcg
    68761 gcaccaaaca agtctcccat gttttcaaag ttgaaggcag agacactgtc tcttgaaatt
    68821 ctgttgttca attggtttct gccggaaagg gtatacctga atttaattcg ttcaataaca
    68881 ttccggggac tttgatttcg ttttctaaat ggaaattggt cctgcatgtt tacactcaaa
    68941 tcgggaagta aaaaggttac ctcctttgtt gtaaggtttt gagtaaatct actattcaat
    69001 cccatactga agggagtccc ggtaaatgac ttggtataag agatggaaga gttgagggtt
    69061 ctgttgatgt tgtcttcaat ttgcaaggca ttgttttgat taaatgtgtt tgttgcagca
    69121 tttatgttga ttgagaatct acttgaacca taattttggg gtgtatgaga ccaattaatt
    69181 ctaaagtctt tagatactgc ttcacgtcct tcctcgccca atttcgtaga agtgtaattc
    69241 caccttaagc tgccggtata tttgtatctt ttcttatagg ttgatgaacc attaatccca
    69301 tagctgccgg tggtataggc gtctccggta accgcgaagt tcacataatc attgatagcc
    69361 caaaagaaac cgccattacg gacaaaaaat cctcggcgta cttcttcgcc ataagtagga
    69421 aatataacac cggaagccct ttgatcaggc gccggaaaca ttccaaacgg aaaacctatt
    69481 ggtgtgggaa catcattaat attgagatta aacggacctg ccacaacttt ttccgatggg
    69541 acaacaatta accttcgtgc ctgaatgtaa taatggggat gttcaaggtt gcatgttgta
    69601 tactttgcat ttctaatata aaggtgatcc agttcatctt ttttcacttc atctccatgc
    69661 ataaaaccct cactttcttc ggtaaccact ccggatatgt aagcacgttt cgatttgaaa
    69721 ttatatttga tgcctgtagt ttcatacctc gattcacctt cctgaaaaac cggcttgcca
    69781 atcatttttc ctgttgaatc aataaccgga tttgctatta acaggttatc gttataatcc
    69841 acctcaattc tctcaccggt gagtactatt tcgccatact caatccttgc atttccatat
    69901 aaataaacat gcttggcgtc aacactgaat cgaatggaat ccaccgcgaa atatttaaca
    69961 gttgtttcaa tatctccctt tcggactaca gaatctgcgc taatagagtc attaggagca
    70021 tttagagagc tttggagggt atcagcctga ttcagaatcg gtttttggac aggaatttgt
    70081 gcaaaccctg taaaactgaa tagaaaaaat agcaggaaag aaaaagaagt tcttaaaata
    70141 tacacccgtc ttttcagttt ttaacaaaaa tttacaactt tgtgtaaagt tatttcaata
    70201 acgataaaca tgggaatatt gtgaagaata ttaccgtaat ttcaatactc tccgcaataa
    70261 tcttgctagg ctcgtttact ccacttgaac tcagaaaaat gaaggtgagc aggattgtag
    70321 tggatgcagg tcatggaggt caggatcccg gaactcatgg cgatttttcc aaagagaaag
    70381 atgtcgcatt gaaaattgcc cttaaatttg gcagatacgt agaaacactc ctggatgagg
    70441 tggaggttat ttatacccgc agtgatgacc ggttcctgga actggaacag cgcgctcaaa
    70501 tagcgaacaa gaacggagct gatttattta tctcgattca ttgcaatgct ataccccgga
    70561 ctgaaataca tggaaccgaa acatatgtta tgggtttgga taaagtcgaa gggaatctga
    70621 tggtggccaa gcgggaaaat tctgtaattc ttcaggagga taattacgaa gaagtttatg
    70681 agggctttga tcctaattca cctgaagcca atgcaatctt tgccttatat cagtccgctt
    70741 atttggaaag cagcctgaaa ctcgcgtaca aagtggaaac tcagtttaaa acgagagtag
    70801 gcagatatag ccggggagtg aagcaggctc ccttttgggt attatggaga acctcaatgc
    70861 caagtgtttt gatagaaacc ggctatctta cgaatccaaa tgaagaaaaa ttcttgaatg
    70921 atgagctaaa acaggattat attgcgtctg caatataccg ggcgttcagg gattataaaa
    70981 aagagttgga ttcaatgtaa aagtcatttc gtgaaatata gcaaagaaat taaggttgga
    71041 tttttttcag ttttaactat tgccatgttg tatttgggag tgaactatct caaaggggta
    71101 gatttctttt ccagtgtgag ccgctactat gtgatgtatg acgatgtagg gggattgcag
    71161 gtttcaaatc cggtaaaaat aaatggattt accgttggcc gggtgagtga tataagtatt
    71221 ctgcaagaaa aacagaataa gatactggtg gaaatcgaaa ttaaagagga aataatctta
    71281 ggagatacaa ctttggcttt attggatgtt gaattcctgg gagcagtaaa cattattctg
    71341 gaggttggag atatttcaaa tcccttaagt tccggagata cattacgacc caaattggat
    71401 caaggcctgg aagatttatt acgatcaagt gcgcttcccg ttgccgataa ccttcaggga
    71461 accattagaa aaattaatac atttctggat gacctttccg gtaatctgga acacctcgga
    71521 cctgccttag aaaatatttc cggtgcagcc aggaacgtaa aaacgatcac agccggttct
    71581 aatcaggaaa agctcagcga tttacttgat aatctaaatt caaccgcggt agaattgaaa
    71641 gccacaataa aaaagattga tcccctggtg cagaatttta cacaggtcgc tgattccctg
    71701 aaaagccttc agataaatga aacccttgcc aaaatgaata caactcttga aggcctcgat
    71761 caaaccctaa cagatatccg cgaaggggat ggaacaatat caagattaat taaggaagat
    71821 tcgctatacg ttcaattgaa tcaaaccctg caaaatctcg ataaactgct cattcacttt
    71881 gatgaatttc cgaaagactt tttatccccc ctgggacgca gcagtaagaa aattcaaaaa
    71941 cgaatagaga aagaaggaaa caattagttc aaattgtttt tttgatttaa ttttctcaat
    72001 aataaattca caaaccataa ttggatgaac cttgaattta atcgtaatga ggataagtat
    72061 aaaatgcttg tttctgaatt gaaaaggaaa ttggcggaga ttaaacttgg aggtggcaaa
    72121 tccaaaattg acagtcagca caaaaaaaat aaacttaccg caagagaacg aatcgaatac
    72181 ttagtcgatc cggcttctga ttttctggaa attggcgctt ttgctgccga cggtatgtac
    72241 aaagaagtag gagggtgccc atcggcaggt gtggttaccg gtatcggata catatcaggc
    72301 agacaatgcg tgattgtggc aaacgatgca acggtaaagg ccggagcctg gtttcccatg
    72361 acagccaaga aaaacctgcg cgcccaggag atagccatgg agaaccggct tccaatcgta
    72421 tacctggtgg acagtgccgg ggtattttta ccaatgcagg atgaaatttt cccagataaa
    72481 gagcattttg gccggcaatt ccggaataat gcccggatgt cttcgatggg tatcatccag
    72541 attgctgcca taatgggtag ttgtgtcgcg ggaggtgcct acctgccaat tatgagtgat
    72601 gaggccatga ttgtaaataa aaccggatcc atttttctgg cgggatcata cctcgttaaa
    72661 gcggcaattg gagaagaaat tgataatgaa acgctgggtg gagcaaccac acattgtgag
    72721 atttccggag ttacagataa taaatatgaa acagatcaag acgctcttga tgcaattaaa
    72781 gatattttct caaaattagg gaacttcgat aaagctggtt tcaacagaat taaatccaaa
    72841 cctccaattg aaaatcagga agaaatttac ggacttatcc ctgaggacgg tttaaagcct
    72901 tacgacacca tgcaaatcat cagaaggatt gtcgataatt ccgaatttca ggaatacaag
    72961 ggattatatg gaaaaacaat tatttgtgga accggaagga ttgatggctg gtctgtgggg
    73021 atcgtggcca atcagagaaa agtggtgaaa tccaaaaaag gtgaaatgca aatgggaggg
    73081 gtgatttact ccgactcagc agacaaggca gcaaggttta ttatgaattg caaccagaga
    73141 aaaattcccc ttgtatttat gcaggatgta acaggcttca tggtgggcag ccgcgctgaa
    73201 cagggaggca taattaaaga cggtgccaag atggtaaatg ccgtggccaa ttcggtagtt
    73261 ccaaagttta cttttattat aggaaattcg tatggcgccg gaaattatgc aatgtgtggt
    73321 aaggcgtatg accccaggct gatctttgca tggccatcgg cccaaattgc cgtgatgagc
    73381 ggaaactctg cagccaaaac cctgctccaa attaaaatat cggcatttga agcaaaagga
    73441 aagaaattaa gtaaagaaga acaggaacag catttgaaag aaatcaagga cacgtataat
    73501 tcacaactga acccctatta tgcttcagcc agattatggg tagatggaat tatcgatcct
    73561 ctggagacgc gaaaaattat ctcaatggga atagaagcgg caaataatgc gcccctctcc
    73621 aataaattta atgtgggcgt gatccagacc tagtgttttt ttgcttcttt atttgtaatt
    73681 tttcatccga ctgtggcaaa ggaaaaaatc aataaaaaag aaatcaaggc tttggtatca
    73741 ttgcttgacg atgacgataa agaggtgctt atcaatgttg aacaaaaact gctttcccta
    73801 ggaggcgatg taatcccgct gcttgaatca gaatgggagt ataatttcaa tccgacggtt
    73861 caaagaaaaa ttgaggatat aattcatgta cttcaatttg aaaacttact cgaccggttt
    73921 caccgctgga aaaatcaaaa aggtaaagat ttgttaaccg gcatgtggct ggtagcaaca
    73981 taccagtatc cggacctcga actggagaat ctaaccaagg agctggaaca aatctattat
    74041 gaagtttgga cggaattcaa accggatttg tatccttacg atcaggtaaa ggttttgaat
    74101 ggagtattat ttagcaaact taggttcagt gccaatacaa agaattttca cagcccaagc
    74161 aacagcatga taaacagcgt tttggaaacc aggaaaggta atcccattag tctcgccgtt
    74221 gtttatctct taattgcaag aaaactgaat atgccggtat atggagtaaa cctacccaac
    74281 ttgtttattt tgacattcaa gaataaggag acacaattct atattaatgc atttaacagg
    74341 ggactgatct tttcccggga ggatattgat aattacattt cagaattaca tctcaataaa
    74401 caagacacct tttatgagcc ctgcaccaac ctggatatca tccagcgtgt gctccgaaat
    74461 ttgatggttt cattcgaaaa aatcggagac cattataaag tagatgaggt gaaacggctt
    74521 ttgaactcca ttctctaaaa aaaatctcat tagaattttc ttgtaactca aatatggaaa
    74581 attcatgaaa aaggattatc tattgaaact aacttagcta acgtataaaa aatagttgaa
    74641 tttctggtag tgtctcagtt ggaatcgtta aacgtgtgcc acactttttc gacttgaaaa
    74701 acaatcaatt tcaactaata aagacggaag tgcggcacac gtagatacta agacactacc
    74761 gaatttctgt ttcccttgaa aataatttct atttgaagta acttagctga gttttctggt
    74821 aggcagttaa cccatttctc ttattaaaaa ttaaagcatt atgaaaaccg gaaaatttta
    74881 tttatccttt cttcccataa ttcttcttct gtcttgcatg aatgacagcg acaagaaaat
    74941 ggaagtcatt tcagttgatg tagcagaagc aagaattgat agtattgcaa agaagcttgt
    75001 agccgctgga aaagttgttg ctgaaaagga atttcttatt ccttccccaa tgcgggggtc
    75061 tgtcaaagaa attattgccc gaaacggtct ttgggtagat tcaggagcag ttttaattaa
    75121 actgaaggac gattatttca ggatggattt ggaaaaggcc aaagcggcaa aatcagctaa
    75181 ggaaattgtt ttccaaaaaa tgacgctgga ctatgtggat atcaatgatt gggaaagtga
    75241 aaaaggacaa gacgtcagaa agatgttacg ttcctatagt ggattagaag tagctgaaat
    75301 taatttacag gaggctctgg aggccctgga caatacgagc atcagaaccc cggtgggtgg
    75361 gatagtagat cagttagtgg tgcaggaaga cgagtatctt tctgatggac aactcctttg
    75421 caaagtgtac caccctgcgc acatggaagt tattaccttc ttccctgaag aggaggggat
    75481 ttatttgtat aaggaccagg aggccagcgt ttatattgat gatgccatag aatctgcacc
    75541 tggaatcata aaatgggtag ataataagcc caacgaaaat ggtatgatta aggtattgat
    75601 ttccctggaa ggagataatc ttttacctga caagccggca cgcgtggaga caatttcaaa
    75661 atactgggca ggtatgctta ttcccgttga tggggtttta ttgaaaaacg gaaaacccgc
    75721 tatttataaa gaggtagagg gtaaagcaat ttttcaattt gtggaacttg ggatggctag
    75781 tggccaggaa attgaaatta ccagtgggtt attattaaat gaaaaagtaa taatttccaa
    75841 tttaaacctg cttgaaagta attcgcaggt tacgataaac aattaggaaa attatttttt
    75901 gattatgcat ccggccgggt ttacaaaggc aggatttgca tttcgattca gcaaaagctg
    75961 attaatggca ttatccagat acggcgttct tacatcatgt tcaacctgag gattgtcatc
    76021 aatcgcgcca cggtaaaaaa tcccaaattt tccattttca ggtttcaata aaaagacctc
    76081 aggattcttt cttgccccaa atttattcat ggcatcctgg gcgatgtcag ccagataggg
    76141 aaagtctaaa gtgttagcgt gagatttaat tgcatcaaga ctctctgcgg attccttgct
    76201 gccaaaattt gaattaatca tcagaaatga aattccatgg tccgagtatt ttttggcaag
    76261 tgctcctata cgtgaatcgt aatgcaatga atatgggcat tccgtactat tgaaaattac
    76321 taaaattcct ttggcattcc taaagttgga aagagagtag ttcttaccct ctcgtgcatc
    76381 cttgagggta aaatcttcca catatttttt tccctgggga aatccttgcg cgtttatgag
    76441 taataaaaga aaaaatatcg tttgtctgcc ggtcatagtg tgtaacaaac aacaaagtca
    76501 ggaaataact tgtattccaa ttcgtaaaac cgaacttcat cgtgtctctt tactttggat
    76561 ttcaatatcc cctccttaag catctcaaaa ggaagtattt caatattatc tttgaaagaa
    76621 agatttttcc tgccatagga tttgtaaaga gcttctttgg ctgcccatac aagaatcatc
    76681 tttcccggat ctttattaga atcgaaaatt tcctgctcag acataaactt ggtttctaat
    76741 ttcagtatct tcttattgta atgttcaaga tctatgccaa caggatcgtt gagattcagc
    76801 atggctgctg catagggata actatgagaa attgaaatat gagttttctc atcgataaga
    76861 aagggtttgc catgcttatc tttccaaatt ccattgtagc gaactcctgc atcagaagcc
    76921 aggtacttta gtaaagcccg tgttgcaatc gactctatct ttctgttaaa cacctttatg
    76981 gtagccaggg agtttctaag gtcttcatcc gaaggcaaat cccgcagcag ttcagattcg
    77041 gattcttcaa tttgccatat tccccatcga tattcagaat tctcttcttt atgaattacg
    77101 ggcatgtagg ttcgacttaa ttgggaagcc aatttatttt atttttgcca cttatggaaa
    77161 gttagccatg aaactctgtc tccataattt ctctctaaac tgcttttatt cagcccattt
    77221 atgcccggat agcaaacatt ttgtaacttg tacctgggaa aaagcgatca aatattaact
    77281 cgggatgagt gaaataaact catttaaagt aatagatgaa ggtttttgaa agttcagagt
    77341 agaaaattgg gtagtgcctc agtttggaat cattatacgt gtgccacact ttttcgacat
    77401 gaaaaacaat caatttcgtc taataaagac ggaagtgtgg cacacgtaga tattaatgat
    77461 cttaactgag accactaccg aaaaatggga taaatgcgat aataatgaaa ataactcctc
    77521 ttgaaatcag acaaaaaaca tttgagaagg tattccgcgg tcttgataag gatgaagttc
    77581 aggcatttct gaatacgctc tcacaagagt gggaaaaagt gaatcaggaa tgcaaagagc
    77641 tcaagatccg tttggagacc agtgagaggg aggttcaaaa acttagagaa gtggaaagtt
    77701 ctcttttcaa gactcttaaa acagctgagg atacaggcgc aaaccttatt gaccaggcaa
    77761 ctaaaaccgc ggacctgcac atgaaggaaa ctcagatgaa aacagaaggt ttaatgaatg
    77821 aagcaaaatc caaggcaaag gaactcatgg aggaagctga ggattatgcc cgcaacactc
    77881 tggtaaagat ggaggatgag ctcaagaacc ttgctgatag ctacagggat ttggaaagtc
    77941 agcgggaaaa tttaatcgat gaattagctc gtctcacaaa tgagagcctg gaaaaactca
    78001 acagatcaag ggctaaaatg aaaagttttg atgtcagctc ctatttgaaa aaaacaaagg
    78061 tagagtcatt catggcttcc attaaggaag atcaattagg aaataagact atttctcaag
    78121 aaccatcaca aaaggttcag cataaagcag aaaaatcgcc tgactccggg tctccatcga
    78181 aaggcgaaga acaagaaacc aattctcagt ctttctttga caatatttaa tgagatcaaa
    78241 atgggttatt tccctaaggg gaataaagat taaagccttc catggccttt atgaattcga
    78301 aaaaaaagaa ggaaataatt ttctggtaga tgtgagtgta aagctgaccg atgatcaact
    78361 aaccatttca gatttagatt ctaccgtcaa ttatgaaaat ctgtacaaaa ttgtggtgga
    78421 gcaaatgaaa atccccactg atcttctgga agaagttgtg aaacgaatcg gagaaaaaat
    78481 tctggaagaa tataaaattg tttcccgggt tgaagtgagt ttagccaaat taaatcctcc
    78541 aatgatggat gattgtgccg aagcaaaagt tactataaag ctttcaaggt gagatcattt
    78601 ttctgcagtg aagatgaatc ctctggcttc gtcgccattg tagaaatcaa cctgttggcc
    78661 agccagataa agagttgatt ttttttcaat gtaaattcta atgccttcca gttcaaattc
    78721 atcatcttgg tccttcttcc tgtcgaaccc taataaaaaa gaaacgccgg cgcatcctgc
    78781 tccacgaaca cccacgcgca agccataatc aaccggtatc tttttagagg aaatgatatt
    78841 cttcacttcg gcaacagccc tgtctgtaaa actaactggc attatctttt ctgacatccc
    78901 ttaatctgag tatatttaat tttcagaata attattgcaa atatactact ataaattaag
    78961 ttaatttctt tatggataca ctgattgagt ttgccaaatt attagtgccg gcagggttag
    79021 ttttgtacgc tatgtacctg gtcgtgaaat cttttttgga gaaagatttt gaaaaaaaag
    79081 tagtagatct cagaaccaaa aatcaggaaa ccgtacttcc gattcgtctt caggcttatg
    79141 aaagaatgac tttgttcctg gaaagaataa caccaagcaa tttgatgccc aggataaaaa
    79201 ccggcaccct taatgcaaaa gagtttcaac aagttttact taatgaaatc agagctgaat
    79261 ttaaccataa tgctgctcag caggtttata tgtcgcagga agcctgggac atgataaaga
    79321 acgccaagga ggatttaatt gtaaccataa ataacagcgc ggcacagtta tcggatgaag
    79381 catcggaaag tgatttaagc aggaagattt ttgagacaac catggaaact gaactggatg
    79441 caataggaaa tgcccttcgg ttcattaagg atgagattca gcaatatttt taattagcaa
    79501 tggaaaagga gggtttcggt aacgagcatt ttgaaaaagc agcggagaag gcaagaaaaa
    79561 ttgcgggcaa caaagaccgg cttatagatc tggtaacagg agtaacacaa aaaattacgg
    79621 ctgaattaaa aggtaaattc ggcgcggcaa gacttaagga aaagctgaat atcctcgtcc
    79681 gcatgattag agcctatgta aacggtgaat acaaaatgat gccgtggaaa gccctggttt
    79741 cgctggcggc cgcattgatt tattttatta cacctctgga tttgattccc gactttattc
    79801 ctgttacagg cttcttagat gatttcacgg ttatcatctg ggttttcaga tctttccagt
    79861 cagagattga cgaatttatc gaatgggaaa aggacagtat aaatacctct ggtggagaat
    79921 aaggttgcga taatagctgg agcatcgggt ttaatcggag ggcatctcgt gcagctcctc
    79981 ttgaattcag attacgaatc gattatctca gtttccagga ggaaactgga tctggagcac
    80041 ccaaagctgg ttcagaaaat cgtaaacttt gatcagctcc atacgctcga aaatgaactt
    80101 gcagcggatc acatattttg ttgcttgggc tctacaatca gaaaggccgg ctcaaaggaa
    80161 aagttcatcc tttacgacta tacctatcct gtggagttgg ctactattac ccgaactaat
    80221 ggagcctcac aatttcacat tgtttcatcc cttggagcaa aaaagaaatc cctgtttttt
    80281 tacaaccggg ttaaaggtga tgtggaagaa gcaatttcaa aaatggattt caggtcgata
    80341 catatttatc agccatcatt attattggga aacagggaag aattccggac tggtgaaaaa
    80401 atgggcgagt atatttccta cctcttttat cccctatacg ggttgattaa aaattacaaa
    80461 cctgtaaagg catccaaagt agccaatatc atgtttgcta tggcccaact agatcatcct
    80521 ggattacatt tccattattc taaagaaatc caatcatatt aatcatgatt acagttattc
    80581 agcgggtctc agaagcgaat gtggcgattg agggaaaagt aaccgctcaa atcggacatg
    80641 gattgttatt attggcaggt tttgaggaag ctgataccga agctgattta gagtggatgg
    80701 gctcaaaaat tgtgaatctg cggatattcc cggatgaaaa tgaagttatg aataaaagta
    80761 ttatggattc cggtggagat attttggtag tatcgcaatt cactttgcat gcaagcacaa
    80821 aaaaaggaaa caggccctcc tatagcaaag cagccaaatc tgaagtggct attccattat
    80881 ataatacttt tctgaatcaa cttgaaaaag tattgggaaa gaaagtagca agtggtgaat
    80941 ttggcgctga tatgaaagtt taccttacca acgatggtcc cgttacaata attatagatt
    81001 caaaagatag taaataatga aagaaattac attgagagaa gcccaaatgg aagttgacaa
    81061 atgggttact accaagggag ttcgttactt caatgaactt acaaacatga ctatcctaac
    81121 tgaggaagtt ggtgaagtgg cgagaataat ctcccgcaag tatggtgacc aatcctttaa
    81181 gaagcgcgag cagaaagcaa acctggatga tgaattggct gatgttttgt gggtattgat
    81241 atgtctggcc aatcaaacgg gtgtggacct tacggatgcc tttaaagata acttgaataa
    81301 aaagaacaaa cgggatgcaa ctcgtcatca aagaaataaa aaactgaaga aatgaaaagt
    81361 caccaactgt taattattat gattttgccg tttgccggct gccaggaaaa atcgggaaca
    81421 atcaatgatg tttacgtaag tgaaatggaa tcctggaagg agcgccggct ggaagccgtc
    81481 accggagcag aagggtgggc aacattgatc ggtttgcact ggctttctca aggggaaact
    81541 actatgggat ctgattcttc ccgtacgatt attttgcctg ctgaagtgcc tgcatatctt
    81601 ggttcttttt ttgtccaggg agagcaggta attttttcag ccgcggataa ttccctgatt
    81661 accgttgccg attctaccat tactaccatc catatagact cgaacaggaa tccggtattt
    81721 gaatgggaaa ctttttcctg gtatgtaatt aaaaggtcgg agcgtttggg attgcgaatg
    81781 cggaatacca ggtctccgga agttttgaat tttaaaggat tgaattattt tccattggat
    81841 ccggagtgga ttgtaaaagc acggtttgaa ttgcatgaac cgccaagaac taaaattatt
    81901 caaaccgtat tggacatgac tattgagtat ccaaatccag ccacgctctt tttcgaatat
    81961 cggggtgaag aatattccct ggaggcattg gatgaaggag gtgataaata ctttctcatt
    82021 ttttcagatg aaaccagtgc cgtggaaact tatggtgccg gcagatatat gtatatctcg
    82081 aaatctccag ttgacggata tctgaccctg gatttcaata aatgttacaa tcctccatgc
    82141 gcatttacgc cctatgcaac ttgtccaatt ccgccacctg aaaaccaact taagatttca
    82201 ataaaagcag gcgagaaaga ttatcactaa tttttatgga atccggctga taacgcggat
    82261 catcgcagag aaaaaatggt tcagggaaac ttctgcataa atcggcctaa tctgcgggag
    82321 attttcttaa tcccgctgat atcgcagatc acctttagta ttgtattaag cttaaaggca
    82381 cagccttatt attactatca cctttttctt ttttagttct tttttctttt tgaagggatt
    82441 gtgaaattgt ggataacgtt aacaactgac tgaggcgatt taagcagatt aacaggttag
    82501 agacctattc tgcgattcaa tcctcagtca gttttctaaa caagcatcaa atagaaattt
    82561 aggttcgagg cctattatta aggttttgaa agtgtttaga agcgttaacg gataaacatc
    82621 aatttatcta aagacaaatt taatggaaac tgtttacttt ttggggattg acatcgcaaa
    82681 gaaaaagttt gatggcgccc taaccgtgga cggcaaggcc tttcaccaag ttgaatcagg
    82741 aaatacctgc aaggaaatca aagccttgtt caaggatttg aaaaagaaat tttccatatc
    82801 gtattctcaa ttggtagtat gtatggaaca caccggcatt tattgccgcc cattactgga
    82861 gtttttgaca gagaatcaca tcaaagtatg cgttgaatcc agtatccaga tcaagagatc
    82921 ccagggtatg gtgcggggga aaacggacaa aattgacgct cagcgtatat catcttacgc
    82981 ctatagaaac cgggaaaatc tccgtttttg gactccccaa agattgatca ttcaaaagct
    83041 aaaggcaatg ctggtaaccc gggaacgcct cattagagtc aagactgaac tctacgtacc
    83101 cataaacgaa tgcgaagaat ttattgaaga atctatccac caaatgatga acaaagcttg
    83161 ccagaacaca atcaatagaa tcgaacgaga tcttgacaat attgaaaaaa tgatcgatga
    83221 tctggtaaca gaagacgctc aactaaacca ccagtatcag ttagctactt ccgtcacagg
    83281 aattggcaag attacagcac tacatatgat tgtctctacg ggggagttta ctcgaattca
    83341 agaggctaaa aaatttgcct gctattcggg cgtagcgccc tttgaacaca gctctggaag
    83401 ctctatacga ggaaagacaa gggtgtcgaa gatggccaac atgacgatca agaagttgtt
    83461 acaccttggc gccatgtcag cagctcgata tgatcatgaa cttcaggcct actatcaaag
    83521 gaaggtatta gagggaaaaa acaaaatgag tgttatcaac gctgtgcgta ataagttgat
    83581 aagtcgtgtt tttgcctgca taaaaaacaa cagaaaatat caaaagattt acaaacatgc
    83641 ccttgcataa aacatagaaa tcgcaga
//
LOCUS       DASWHX010000047         5822 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_1509464, whole genome shotgun sequence.
ACCESSION   DASWHX010000047 DASWHX010000000
VERSION     DASWHX010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 5822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5822
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1509464"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1231
                     /locus_tag="VGA21_03360"
     CDS             <1..1231
                     /locus_tag="VGA21_03360"
                     /inference="COORDINATES: protein motif:HMM:NF019311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HEX9649573.1"
                     /translation="IPETGFPGVDWESCMTMNDNWGYNKNDNNWKSTKELIRNLTDIA
                     SKGGNFLLNVGPTAEGIFPEESVNRLKEIGAWMEINGESIYGTQASPFTGIKWGKVTC
                     KSVNGSSTLYLHVFDWPEDGKLQLHGITNRPTSAKLLAGGGRIIAKRMEAGIEISLPA
                     QPPDSINPVIKLSLSGTLDVINPPGIITRDYIFIEKLTVVLEMDEQFDLRYTLNGPLP
                     TIQDPVYDSLGIIIEETTNLKVRKFNYETAMSDVTEKLFTRVQPNPSLNTLRRTPGVL
                     LNYYEGNWDVLPDFNKLDHIEQMESQNFGVSLKLREEYFGLEFLGYVNVPERDVYYFY
                     LSSDDGSNLYIDAKLIVENDGLHGMKEASGAVALEAGPHLIKVIFFEKTGGDDLKVEW
                     HSSRFNKQTIADSLLFY"
     gene            complement(1220..1777)
                     /locus_tag="VGA21_03365"
     CDS             complement(1220..1777)
                     /locus_tag="VGA21_03365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649574.1"
                     /translation="METLFSIAELPDGIVAGLLSGVFASAIVSLVTYRLVLGRNRKKL
                     KEKFSLPAGEYNCYSFEKILPDIEDETSANFKRSITPNGGKADIVYESENILKITLKE
                     RDGYEWSGKIFMQDETVGNIVWRYTNLPLQRDKEWHWFGLKKLLVREEEHFVYLYIIG
                     EVSEGFGNEVLMKKKIRGVRNGLVK"
     gene            complement(1964..4729)
                     /locus_tag="VGA21_03370"
     CDS             complement(1964..4729)
                     /locus_tag="VGA21_03370"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX9649575.1"
                     /translation="MKRAFILIFFIPFFISAQKKPLDHSVYDIWKSINNPSISMNGEW
                     VVYSIDPEQGDGDLFIQQPSKNSTSVFPRGKRGRISFDNNHIIFTIIPHADTVLNMKR
                     RKVKKDEMPRDSLGIFNTKSISVTKIPGISDVKVPEKWSGIIAFRIFPAQPEGNGKPK
                     SKKVNGEGLGLIIMDLNTGRQDTVPGVTDYVFAREAKELMMISGGKDSTMLPGVYRWN
                     FSNNQLEPIFRSKGKYKSVTLDEKGGNGAFIADLDTTKAKVRPWNLYVWKKGVDSAKL
                     VAKNGMATFPQGWIISDNANLDFSKSGNRLFFGTAPPPLHPDTTKLPEELAVVEVWNY
                     KDQRLYTQQNISLNQDKLRSYTTMFNINDGKISQLEQEDMNQVRMTREKDMDFAIGLA
                     GNEYQKFMSWQGRTYSDIYLVNMKTGSREKILTKKSGFFNLSPSAKYIYWYSEIDSAH
                     FTYSIATKKVSQITNNNSVLFYNELNDVPDHPGSYGNGGWTKDDKEILIYDRFDIWSV
                     DPENKRTPVNLTKTGRSNNTRYRIRDLDEELTYIDPGTPLLLESFNEVDKSEGFYSFS
                     LKDLKAPKELIKGNFSLSVVEKSQGSDGLLFTRETFQKFPDLQFSDLSFLNPKQITET
                     NPQQQEYLWGTVESYYWTSLDGKKLEGLLFKPENFDPSKKYPLIVNFYERSSNGLNSH
                     RIPYAHRSTINYPYYLSNGYVIFNPDIPYRIGYPGESAFNSVIPGVSSLIDQGFVDKE
                     RIGVQGHSWGGYQIAYLVTKTDIFKCAESGAPVVNMISAYGGIRWGTGLSRMFQYEHT
                     QSRIGGTLWETPVRYIENSPIFFIDKINTPLLIMHNDNDSAVPWYQGIEFFVALRRLG
                     KPAWMLNYNGEPHWPLKPANRKDFVQRMSQFFDYYLKDAPMPVWMDEGIPAINKGIDY
                     GLEPSKQ"
     gene            complement(4809..5321)
                     /locus_tag="VGA21_03375"
     CDS             complement(4809..5321)
                     /locus_tag="VGA21_03375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649576.1"
                     /translation="MTEYDEKRSFKLKPLSKDGIETALNKAEHYRLLNQPLLAESICR
                     DILMVESENQKAAIFLLLSLADQFGQSKTPKEALEIAGNLKDPYHQKYYGGIVHERQG
                     MAALSSAIPGSNFDAFEWYLEAMELFEEASAIQPANMEPVMRWNTCARVIMNFNLKQR
                     PKDDSHPMLE"
     gene            complement(5447..5806)
                     /gene="frdD"
                     /locus_tag="VGA21_03380"
     CDS             complement(5447..5806)
                     /gene="frdD"
                     /locus_tag="VGA21_03380"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: protein motif:HMM:NF003977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HEX9649577.1"
                     /translation="MSKRLNTSDPLWWTFFGAGGALSALFLPVLIALFGIAIPLGLIE
                     PPSYEKINSLMSPVLSRLVLFAVLSTSMFHWAHRFRFTLYDGLQLKRFELWIMFICYG
                     TAIVVSIILAIILWNFN"
BASE COUNT         1694 a         1180 c         1126 g         1822 t
ORIGIN      
        1 aattccggaa accggttttc caggagtgga ttgggaaagt tgtatgacaa tgaatgacaa
       61 ttggggctat aataagaatg acaataattg gaagtcaacg aaagagttga taagaaatct
      121 tacggatata gcttcaaagg gaggaaattt tttattgaat gtcggaccaa cggcggaggg
      181 cattttccct gaagaaagcg ttaatcgcct gaaagaaatt ggtgcatgga tggaaattaa
      241 tggggaatct atttatggta ctcaagcgag tccttttacg ggaattaaat ggggtaaagt
      301 cacctgtaag tccgtgaatg gtagttctac gctgtactta catgtatttg actggcctga
      361 agatgggaaa ttgcaacttc atggaataac caaccggcca acatcggcaa aacttttggc
      421 tggcggcggt cgaattatag caaagcgaat ggaagctgga atagaaatta gtttgcctgc
      481 acagccgccc gattcaatta acccggtgat caaattaagt ttaagcggca cactggatgt
      541 catcaaccct cccggaataa ttaccaggga ttatattttt attgagaaac tcacagttgt
      601 actggaaatg gatgagcagt ttgatttaag gtataccctg aatgggccat tgccaacaat
      661 tcaggatcct gtttatgact cccttggaat cataattgaa gaaacaacca atttgaaagt
      721 caggaagttt aattatgaaa cagctatgag tgatgtgacg gaaaaattat ttacccgcgt
      781 acagcctaac ccttcgctca atacattacg ccgtactcca ggcgtattac tcaattacta
      841 cgaaggtaat tgggatgtat taccggattt caataagctg gatcatattg agcagatgga
      901 atctcaaaac tttggtgttt cattaaagct tagggaggaa tattttgggc ttgaatttct
      961 gggttacgta aatgtgccag aaagagatgt gtattatttc tatttatctt ccgatgatgg
     1021 cagtaattta tatattgatg ctaaacttat agtggagaat gacggattac atggaatgaa
     1081 ggaagcctcc ggggcagtcg ctctcgaagc cggaccacat ctgataaagg taattttctt
     1141 tgaaaaaacc gggggcgacg atttgaaagt ggagtggcat agttcgcgat tcaataaaca
     1201 aaccatcgcc gattctttgc tattttacta aaccatttct aacacctctg atttttttct
     1261 tcatcagaac ctcattgcca aatccttccg atacttctcc tattatatac aagtaaacga
     1321 aatgttcttc ttccctaacc agtagcttct tcagcccaaa ccaatgccat tccttgtctc
     1381 tttgcagagg caggtttgtg tatctccaca caatatttcc tacggtttca tcttgcataa
     1441 aaattttgcc gctccattcg taaccgtctc tttctttcaa tgttatcttt aaaatattct
     1501 cactctcata aactatatct gctttacctc catttggtgt aatagatcgc ttaaaattag
     1561 ccgatgtttc atcttcaatg tcggggagaa tcttttcaaa actataacaa ttatattctc
     1621 ccgccggaag cgagaatttt tccttcaatt tctttctatt tctacctaat accaaccgat
     1681 aagtgactaa tgaaacaatc gcactggcga atactcctga tagcaatccg gcaactattc
     1741 cgtcaggcaa ttcggcaata ctaaataatg tttccataaa aaaaagttag gttgaatttt
     1801 acagagctcc aattacctta ccagcctgcc tgataagcgc gtcatttttt tcaaaatgtg
     1861 gatttattaa aaagtatggc tataccttcc ttaaataaaa aggtggcggg ttaagaaaat
     1921 tatttaataa gattgctatt atgaaaactt ttttcccaaa atgctattgt tttgatggtt
     1981 ctaaaccata gtctattcct ttattaattg ctggaattcc ttcatccatc caaaccggca
     2041 tcggagcatc tttcagataa taatcaaaga actgcgacat ccgctgtacg aaatctttcc
     2101 ggttagccgg ctttaacggc cagtgcggct ctccattata gttcaacatc caggctggct
     2161 ttccaagacg tcgtaatgct acgaagaatt caatgccctg gtaccagggt acggctgaat
     2221 cattgtcgtt atgcataata agaagcggag tatttatctt gtctataaaa aaaatgggtg
     2281 agttttcgat gtatcgaacg ggtgtttccc acaaagtacc gccaattctg ctttgggtgt
     2341 gttcatattg aaacatcctg cttaacccgg taccccaacg gatgccaccg tacgcactta
     2401 tcatattcac cacaggtgct cccgattctg cacacttgaa aatatccgtt ttcgttacca
     2461 ggtacgctat ttgataacca ccccagctat gaccctgaac tcctattctt tctttatcaa
     2521 caaatccttg atcaattaaa gaggagactc cagggattac tgagttaaag gcgctttctc
     2581 caggataccc tattctataa ggaatatcag gattaaatat cacgtagcca ttgctcaaat
     2641 agtagggata gttaatagta gaccggtgag cgtatggtat tcgatgagaa ttcaacccat
     2701 tggagctacg ttcataaaaa tttactatca ggggatattt ttttgaagga tcaaaatttt
     2761 ccggtttaaa taacaagcct tccagttttt ttccatccag agaagtccag taataggatt
     2821 caacggttcc ccacaaatac tcctgttgtt gtggattggt ttcagtgatt tgtttagggt
     2881 tcaggaatga taaatcagag aattgaagat cagggaactt ttgaaaagtt tcacgtgtga
     2941 acaacaatcc atcacttccc tgactttttt cgactactga taagctgaaa tttcctttta
     3001 tcaattcttt gggagctttc aggtctttca aagagaatga ataaaaccct tcggacttat
     3061 ccacttcgtt gaatgactcc agcaataagg gcgtacccgg atcgatgtat gtgagttctt
     3121 catcaagatc tctgatcctg taccgggtat tattacttcg accggtttta gtaagattta
     3181 ctggtgttct tttattttca ggatctacag accagatatc aaagcgatcg taaatcagaa
     3241 tttctttatc atcttttgtc caaccgccgt tgccataaga gcctggatga tcaggtacat
     3301 cattcagttc attataaaaa agtaccgaat tattatttgt gatttgggaa actttctttg
     3361 tagcaataga ataggtaaaa tgcgctgaat caatttcaga ataccaatag atatattttg
     3421 cggaaggcga caggttaaaa aatccggatt ttttggttaa gattttttcc ctcgatcccg
     3481 ttttcatatt caccaggtaa atgtccgaat aagtccttcc ttgccagctc atgaattttt
     3541 gatattcatt gccagccaat ccaatggcaa agtccatatc cttttcccgt gtcatgcgta
     3601 cctgattcat atcttcctgt tcgagctggg agatttttcc atcatttata ttaaacattg
     3661 tagtgtaaga tctcagctta tcctggttaa gacttatatt ttgctgtgta tacagacgtt
     3721 gatctttgta attccacact tcaactaccg cgagttcctc gggtaatttt gttgtatcgg
     3781 gatggagcgg gggaggagct gtaccgaaga acaaccggtt tccactcttt gaaaaatcaa
     3841 gattagcatt atcacttatt atccatcctt gaggaaaggt tgccattcca tttttcgcaa
     3901 ccaattttgc agaatccact cctttcttcc atacgtataa gttccatgga cgaactttcg
     3961 ctttagttgt gtccaaatca gcaatgaacg caccattgcc gcctttttca tcaagggtaa
     4021 ctgatttgta ttttcctttg ctcctgaaaa tcggttccag ttgattattg ctaaagttcc
     4081 atcggtagac accgggcagc attgtactgt ctttccctcc ggaaatcatc atgagttcct
     4141 ttgcttcgcg ggcaaacacg taatctgtta ctcctggcac agtatcttgt cttccggtgt
     4201 tcaggtccat tattatcaac ccaagtcctt cgccattaac tttttttgat ttgggttttc
     4261 catttccttc aggttgagca gggaatattc tgaaagcgat aattccgctc catttttccg
     4321 gaacttttac atcgctaatc ccgggaatct ttgttaccga gattgatttt gtattgaaaa
     4381 tacccaggct gtctctgggc atttcgtctt tcttcacttt tcttcttttc atattcagaa
     4441 cagtgtccgc atgaggaatt attgtgaata tgatgtgatt attatcaaac gaaattctac
     4501 cacgcttacc gcggggaaaa acggaagtgc tgtttttgga aggttgctga ataaataaat
     4561 caccatctcc ctgttcagga tcaatcgaat acactaccca ctcgccattc atggagatgg
     4621 atgggttgtt tatggacttc caaatgtcgt atacggaatg gtccagaggt tttttttggg
     4681 cactaatgaa aaatggaata aaaaagataa ggataaaagc acgtttcatg ggaagggaat
     4741 taaattgaat attttacaaa cacttcataa gagaaatctt aagcctatca aaatatggaa
     4801 atgaaatatc actccaacat ggggtgggag tcatctttag gacgctgctt caggttgaaa
     4861 ttcataatta cccgggcaca ggtattccac cgcataaccg gttccatatt ggcaggttga
     4921 attgctgatg cttcttcaaa caattccatc gcctccagat accattcaaa ggcatcaaag
     4981 tttgaacccg gaatagcaga acttagagca gccatacctt gtcgttcgtg tacaattccg
     5041 ccgtaatatt tttgatggta aggatctttc agattgccgg ctatttctag tgcttccttt
     5101 ggagtttttg attgcccaaa ttgatcagcc agggacaaca gcaaaaaaat tgccgccttt
     5161 tgattttcag attctaccat aaggatatcc ctgcagatac tctccgctaa taggggctga
     5221 ttaagcaggc gataatgttc agctttattc aaagcagtct ctattccatc tttggaaagg
     5281 ggcttcagct tgaaagatct cttctcatca tattctgtca taatttctga atttagtaat
     5341 ttaaacgcaa cggaatggca aattcaactt ttgcccaaca agtacggtat ttatgcatat
     5401 aatttacgag gtgtggaacc aatttgcaac accgttattg atgtattcaa ttgaaattcc
     5461 ataagattat tgccagaatg attgatacga ctattgcggt cccgtaacaa atgaacataa
     5521 tccaaagctc aaaccttttc agttgtagtc catcgtatag ggtaaatctg aatcgatgtg
     5581 cccaatgaaa catagaagtt gacaaaacag caaaaagtac gagtctgctc aaaaccggac
     5641 tcattaacga attaattttc tcataggagg gaggctctat taatcccaat ggaattgcga
     5701 tgccaaacaa tgcaatgaga accggaagaa aaagcgctga aagtgctccg cctgctccga
     5761 agaatgtcca ccacaaggga tccgatgtat ttagtctttt gctcattttt caacttattt
     5821 ta
//
LOCUS       DASWHX010000048         3840 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_32415, whole genome shotgun sequence.
ACCESSION   DASWHX010000048 DASWHX010000000
VERSION     DASWHX010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 3840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3840
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32415"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..785)
                     /locus_tag="VGA21_03385"
     CDS             complement(<1..785)
                     /locus_tag="VGA21_03385"
                     /inference="COORDINATES: protein motif:HMM:NF027232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEX9649578.1"
                     /translation="MKRLLPILLLFLFSSLIFAQNQLTSQTLSGLKLRNIGPAIMSGR
                     IVDLAVVESNPITFYVATATGGLWKTTNNGITFTPVFQNEGTHSIGAIAVHQKSSKFV
                     WVGTGERANRQSSSWGDGIYLSSNSGRSWKNIGLKDSHHIGRIVLHPEDTGVAYVAAM
                     GHLWGPNPERGLYKTSDGGKSWVQLLYVDENTGVVDVAMDPSDPNILYCATYQRRRTP
                     YGFDGDGPGSALYKSADAGKTWRKIHNGLPAGEYGRIGISIYR"
     gene            complement(1050..3008)
                     /locus_tag="VGA21_03390"
     CDS             complement(1050..3008)
                     /locus_tag="VGA21_03390"
                     /inference="COORDINATES: protein motif:HMM:NF022623.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HEX9649579.1"
                     /translation="MASMKLMFQYLLTAIVLVQLGCIPEEKILFSLSSSEVMQIRDAR
                     FVQVSSHDTTGGNNDRINIHAGKTAEIFNVSGAGLITRIWITVDSRDPHFLRRILLRM
                     YWDDESYPSVEVPIGDFFGSPFEYRHFAAENIGMTSGGYYCYFPMPFNNRAKIEIVNE
                     TGEEIYAFYYQIGYYQMPAQFDDNTGYFHAYWNREARTQNDNNYIALKAVGNGQFAGI
                     NFSSQSYNSSLFYLEGDEMFYVDGEEEPSIYGTGMEDYFTSGWYFKNGEFAADYHGLV
                     MKDGEKGRITAYRHHIRDAIPFKDSIEISFEHGHANEQAVDFSTVAFWYQIEPHRKFP
                     AIPVAGQRIPLRRPVPNGAIEAESLVGGGNNTLAVEDMSEYGSDWSGNKQVVLSPGAN
                     TSVTLTLSNLREKTYDVDVYGTKGHGYGKFEIRTSGKNKKVMLDGTGEELLPLDKVTI
                     EDVPVQDGKILLEFISEHEGNKAGIDAFVLTPIRGYIPEWYMIGPFPNPRESDYVRHG
                     LDSVYGPEKEIDLKAKYIGAEGQQVSWERINGKNAGYGMALWSRYNPYEFVVCYALTY
                     VFSPEEKEVSLLFSSDDGSKIFLNNEEIFRFMEVNIAYPDQFEIPLNLKAGWNKLLIK
                     VENNFGGYAFFARLLDPKNEHKTSINAR"
     gene            3169..>3840
                     /locus_tag="VGA21_03395"
     CDS             3169..>3840
                     /locus_tag="VGA21_03395"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9649580.1"
                     /translation="MNILLAIAFIFNCHGPDAKQLLDSARKARNTQPAIAINISMYAL
                     EISGKSGDSVLMAEAYRLLGLLHSDLGDNREAMVHYRKSIDISEAINYGEGIRKAYNN
                     LGVIQLEMGKYQESLNSHYKSLRMKEEANDSLGISYSLSNLGLLHHKFKDYQNAEKFF
                     LKSIRIRERFERTDLLAKGYNRLADLYFEMNLDDKALAFWNKSYQINQEIMNPEMLSE
                     NYLGFA"
BASE COUNT         1122 a          832 c          714 g         1172 t
ORIGIN      
        1 ttgcggtaga tgctgattcc aattcttcca tactcacccg caggtaaacc gttgtgaatc
       61 ttccgccacg tttttcctgc atccgcagat ttatataaag cactgccggg gccgtcgcca
      121 tcgaaaccat agggtgttct tcttctctga taggtggcgc aatacaaaat gttcggatca
      181 gagggatcca tggcaacgtc tactactcct gtattttcgt ccacatataa aagctgtacc
      241 caggattttc ctccgtcaga agttttataa agtccacgct cgggattcgg cccccaaaga
      301 tgtcccattg cagcaacgta agcaacaccg gtgtcttccg gatgcagcac tattcttcct
      361 atatgatgag aatctttcaa gccaatattt ttccagcttc tgccactatt ggaggaaaga
      421 taaattccgt caccccagga ggagctttgc cggttggctc gctcaccggt acccacccac
      481 acaaattttg aactcttttg atgaacggct atggctccaa tagaatgtgt tccttcattc
      541 tgaaaaacgg gagtgaaagt tataccgtta ttagtggttt tccataaacc tcctgttgct
      601 gtcgctacat agaaagtgat tggatttgat tctaccacag ccaaatcaac tatccgcccg
      661 ctcattatag ccgggccaat gttgcgaagc ttaagtccgg aaagagtttg tgatgttaac
      721 tgattttgag cgaatatcag actgctgaaa agaaagagaa gtaatatggg taataaacgt
      781 ttcatgggaa taacatttta tggaaaggta atttaatgta ataaggttaa gtttcataga
      841 agcgtttcga tgaatggcat tagaaactta tgaaaccaaa ctcaattccc catcagtgat
      901 aaatacacaa atatttgcaa agtcagttgc cggtcttccg ctactccttc gttgttcgtt
      961 ttcctatctc atccgtaatt tatttccctg agcattttgc cttttttaat ttctgcccga
     1021 gccgtggatt ttttttattg aatttatgct tatcgggcat tgatactggt tttatgttca
     1081 ttctttggat cgagcaacct ggcaaaaaag gcatagccgc caaaattatt ttcaacttta
     1141 attaagagtt tattccaacc tgctttcaga ttcagcggta tttcaaactg gtccggataa
     1201 gcgatattaa cttccataaa tctaaatatt tcctcattgt tcaaaaatat ttttgaacca
     1261 tcgtcgctgc taaataataa tgacacttct ttttcttcag gtgaaaaaac ataagtaagt
     1321 gcataacaaa ctacaaactc ataggggtta taccggctcc ataacgccat gccatatcct
     1381 gcatttttac catttattct ttcccaggaa acttgttgcc cctccgctcc aatatatttc
     1441 gctttcagat caatttcctt ctcgggccca tacacagaat caagtccatg ccttacataa
     1501 tctgactccc tgggattcgg aaatggtcca atcatatacc attccggaat atatccgcga
     1561 atgggtgtta aaacaaaggc atctatcccg gctttattcc cctcgtgctc agaaataaac
     1621 tcaagtaaaa ttttaccatc ctgaacaggc acatcttcaa tagtgacctt atccaggggt
     1681 agtagttctt caccagtacc atcaagcatt acctttttat ttttgccaga agttctgatt
     1741 tcaaattttc cataaccgtg tcctttcgtt ccatacacat caacatcata tgttttctct
     1801 ctcaaattac tcagggttag cgtcacggat gtgtttgcgc caggtgataa tactacctgt
     1861 ttattgccgc tccaatcact gccatattct gacatgtctt ctactgccaa agtattatta
     1921 cctccgccaa ccaaagactc cgcttcaatt gctccatttg gcacaggcct gcgtaaggga
     1981 attctttgac ctgctacagg gatagcaggg aatttccggt gtggttctat ctggtaccag
     2041 aaagctacag tgctgaagtc tactgcctgt tcattggcgt ggccatgctc aaatgaaatt
     2101 tcaatactgt ctttaaaggg aattgcatcc ctgatgtgat gccggtaagc cgtaattcta
     2161 cccttctcgc cgtctttcat tacaagccca tgataatcag ctgcaaattc tccgtttttg
     2221 aaataccagc cactggtaaa atagtcttcc atgccggttc cataaattga aggttcttct
     2281 tcaccatcga cataaaacat ttcatcccct tcaagataaa ataaacttga attataggat
     2341 tgactgctga aatttattcc tgcaaactga ccatttccaa cagctttcag ggcaatatag
     2401 ttgttgtcat tttgagtgcg tgcttctctg ttccaataag cgtgaaaata tccggtgtta
     2461 tcatcaaatt gggcaggcat ttgataatat cctatttgat aatagaaggc ataaatttct
     2521 tcaccggttt catttacgat ttcaattttt gcccggttat taaatggcat cgggaagtag
     2581 cagtaatatc cgccgctggt catgccgata ttttcagccg cgaaatgtcg gtattcaaac
     2641 ggactcccaa aaaaatctcc gataggaact tcaacggaag ggtaactttc atcatcccaa
     2701 tacatacgaa gcaaaattct tctaaggaag tgagggtcgc gggaatcaac ggtaatccag
     2761 attcgggtga taaggccggc tccggaaaca ttaaatatct cagcggtttt gccggcgtgt
     2821 atattgatcc gatcattatt gccgcctgta gtatcgtgac ttgatacctg aacaaacctg
     2881 gcatcacgga tttgcatcac ctccgatgaa cttaaactga aaagtatttt ttcctcagga
     2941 atgcaaccta actgaacaag gactattgca gtcagaaggt attgaaacat caatttcata
     3001 ctggccatgg caaactaaat taagaataat tgtagaattc attccgtcag aatctcctga
     3061 attcttcata aaaacagtac aagtgtaagg tcaccagtcc ctattagaat aatataatag
     3121 tacataatat aaattacatt atttttgatt tctatttgaa aaaatcaaat gaacatacta
     3181 ttagctatag catttatatt taattgtcat ggtcctgatg cgaaacaatt acttgacagt
     3241 gccagaaagg ccaggaatac tcagccggcc atagccataa atatttcaat gtatgcactt
     3301 gaaatatcag gaaagtccgg ggattctgtc ctaatggcag aagcgtaccg tcttctggga
     3361 cttcttcata gcgatttagg tgacaacagg gaggccatgg tacattatcg gaaatcaatt
     3421 gacatttcag aagctattaa ttatggagaa ggaattcgaa aagcttataa taatctgggc
     3481 gtgattcaat tagaaatggg taagtaccag gaatctttaa attcccacta taaatccttg
     3541 cgaatgaagg aggaggcaaa tgacagtctg ggaatcagct attcactgag taacctgggg
     3601 ttgctccatc ataagttcaa agattatcag aatgcagaga aattcttttt gaaatcaatt
     3661 cgaattcgtg aacgctttga acgtaccgat ttgctcgcca aaggatataa tcgattagct
     3721 gacctctact ttgaaatgaa tttggatgat aaagctcttg ccttttggaa caagtcttat
     3781 caaatcaatc aagaaattat gaatccggaa atgctttcag aaaactatct tggatttgca
//
LOCUS       DASWHX010000049        39460 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_32923, whole genome shotgun sequence.
ACCESSION   DASWHX010000049 DASWHX010000000
VERSION     DASWHX010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 39460)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39460)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39460
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32923"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            628..1842
                     /locus_tag="VGA21_03400"
     CDS             628..1842
                     /locus_tag="VGA21_03400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649581.1"
                     /translation="MKKSINILILLVLISCQVQEKLETNPGTIKFSMQSVVRNEGGRI
                     TETPTPASVLISIEDESGNTVEDNKKLTLYSLGQGFVSESLQLIPGSYKLTQFLILNE
                     DDGVIYATPQEGSELAEFVNDPLPIEFTISEDENTLVTPQVLAVHPEDTPESFGFASF
                     GFEVVAPRLKKVIYTSIFGIHIRHFSYNGELLAEIAEKGCQTCSSGNFEYYGSVNFEY
                     SPDKKLIKRTYVNKYGVVTPYFINEYSGGLLVKRKIFGPSYEYEQQYTYDSNTHLISM
                     TESTGRLHEFELSLESDKLIDKLFLNFQIPESLRFITEFSFDGKRATAFPITGSEEII
                     DIYGYPIGQNVIGIRQKDLNTITGEVNYECYASIQYEYNSLGLPVKATVSNPDGCIFE
                     IEDGTVIEFFYE"
     gene            2007..2399
                     /locus_tag="VGA21_03405"
     CDS             2007..2399
                     /locus_tag="VGA21_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014774019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HEX9649582.1"
                     /translation="MTFEKLSKCEEAIGKAIVTSAFKVHSELGPGLLEKIYEVCLAHE
                     LRKAECEVTRQLEIPIHYDGITFDEGLRLDILVAKKVIVEIKAVDQVNPVWQAQVLSH
                     LKLTGLRLGYLINFNVPLIKDGIKRIIL"
     gene            complement(2623..3678)
                     /locus_tag="VGA21_03410"
     CDS             complement(2623..3678)
                     /locus_tag="VGA21_03410"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015264180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEX9649583.1"
                     /translation="MTLKRRLNRTVGVVGTGSFGTAISNILAANSDVLLYGRNAEGVD
                     EIRKARVSAGQQLNEQVRLTSDIAEIATTCSVIFPIVSSAGFREMMQGLGPLLHPYHI
                     LIHGTKGLDIQMTEKEIQNRRKFDVEKIKTMSQVILDESPVVRVGCLAGPNLARELAD
                     KQPAATVVASHFEEVIHEGQRLLRNDRFRVYGNEDLRGIELAGVLKNIIAIASGALSG
                     LGLGENARALLVSRGMVEMIHLGKALGGEVHPFLGIAGIGDLVATCASNLSRNFTVGY
                     RLARGETLQQILSTMEEVAEGVNTVHIMKFAADYLKVRAPITEALYQVLFGKLSVERA
                     LEGLMRFPLHADIDFLD"
     gene            complement(3653..5362)
                     /locus_tag="VGA21_03415"
     CDS             complement(3653..5362)
                     /locus_tag="VGA21_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009583547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEX9649584.1"
                     /translation="MDNTIIKKPGKTRKKGFEPIHSGPRDWSVFQLSRNKKTFIREVT
                     ELAIKRIKKQTPTKESLIEELETTLYREKLRIKRYPWKVDPPDEAVFWEGVKQKLLEL
                     SSTTNGRDNKVEEEILQSIVSRYANEISGNFKASSYRVARAIATFGFRRLLNAARIKK
                     FGAIFRRDLTLRDKINIVGHTEQIRKLAKKGTVIMLPTHFSNLDSVLIGWVIHELGLP
                     PFIYGAGLNLFNIGIFAYFMNSLGAYKVDRRKKNLPYLETLKAYSSLAVKKGAHSLFF
                     PGGTRSRSGQIEDSLKLGLLGTTIEAQREIYQDNPGPDARKIFIFPVVINYHFVLEAP
                     LLINDYLRLRGQEKYYVENDNLSNSYNILKFMIKFFTKGSDISVSIGHGVDVLGNYVD
                     DEGNSTDKRGKIIDSREYFVYEGRVTENKQREEEYTRMLAKAIVSEFHISNRVFSSHI
                     IAFVAFEMIKNKYPQFDLFNLLRIPEDEIEIPYEEFRQNVKRLRKKIFKMKKKGIVGA
                     APHLEGDVDEVINHGLSNLGLYHTLRPLKKNKKGNIITMDLARLYYYHNRLKGYDLEK
                     KIK"
     gene            complement(5488..5820)
                     /locus_tag="VGA21_03420"
     CDS             complement(5488..5820)
                     /locus_tag="VGA21_03420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649585.1"
                     /translation="MLIIILKGRKHPTPVNQIRRISLGNKKLLGPIVTGGIIFPLSSL
                     AFLGNISNPFLMMTLMIAGLFLLLYGFGGSAAISIETTGGTHHYFIRKTGKNLEDFIK
                     YYNSEINQ"
     gene            6051..8870
                     /locus_tag="VGA21_03425"
     CDS             6051..8870
                     /locus_tag="VGA21_03425"
                     /inference="COORDINATES: protein motif:HMM:NF024974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HEX9649586.1"
                     /translation="MILGLRQLFSFLKKFLIPVLAISIFVTEARATHLRAGEITVQRI
                     SCQELTFRITITVFTDIGPQVTAKFGQGELDFGDGTVELTPRQENPLDLGNDVGYNQY
                     VTEHTYQGNGIYTITYTEPNRNDEILNMSNSVNTKFHLETVIFLDPFLGCNNTPVLLV
                     PPIDRGCTQNAFYHNPGAFDFEGDSLSYEFVIPKSEKGINVLDYVFPNHASFNGMDED
                     GIGPATLTLNPSTGEIVWNSPGQPGEYNIAFKVTEWRIIDGIAFKLGHVTRDMQIIVE
                     DCDNERPEIVVPEDVCVIAGEIVQGVAIGSDPDGDPVKVEAFGGPFEVTPAAVTIPAN
                     PGFVPTPTQLVFEWQTDCNHIRIQPYQVEFKVSDNPPTGPTLVEFGTWNITVVGPPPV
                     LLCANPELMTGRNIRLDWEAYKCDNAETIQIWRRVDSFDFDPGECVTGMPLYAGYELI
                     KIVPATQNFHMDLNLDVGASYCYRLVAVFPAPGGGLSVVSNEVCADPILADAPVITHV
                     TVEVTDEIDGQINVSWTAPFQLDPIQFPPPYRYDVYRAAGLIGETELVLVGSTSDTTI
                     TDSGINTVELPYNYRIYLFDVNDSPVDTSAVASSVRLTPQPLIKEIQLNWIASVPWSN
                     QVQDFPYHYIYRDNADSGDPAKLQLIDSVNVLLNGFTYSDTGFNGVELKETTEYCYYI
                     ETMGSYGNPLIAAPQRNLSQIICTQPSDTIPPCAPLVSIDTIDCENFKLTELCNFNTY
                     SNTISWNEPQECADDIRSYNIYFSRTGQEGSFNLINNITETNFVHDNIPSFAGCYLVT
                     SVDRSGNESDFSEMVCNDNCPNYVLPNVFTPNGDGYNDVFGAFNQTNLPSEDEPGSML
                     PDLSKCPRFVRAVNFMVYNRWGKEVFKATSAGENSLFINWNGKNSDGSDASAGTYYYV
                     AEVTYDVLNPRESVRILKGWIQLVR"
     gene            8874..9272
                     /locus_tag="VGA21_03430"
     CDS             8874..9272
                     /locus_tag="VGA21_03430"
                     /inference="COORDINATES: protein motif:HMM:NF016056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase DCC family
                     protein"
                     /protein_id="HEX9649587.1"
                     /translation="MNKPVILFDGVCNFCNGSVNFIIKRDPKAKFSFCPLQSDEAINL
                     MIPFNMDPRASDTIILLRNNKLYIKSRAALEIIKELTGLWPLLYGLIIVPPFIRNFFY
                     SILAKNRYKWFGKMDACMVPSGEIRNRFIS"
     gene            9325..10197
                     /gene="fabD"
                     /locus_tag="VGA21_03435"
     CDS             9325..10197
                     /gene="fabD"
                     /locus_tag="VGA21_03435"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009185375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEX9649588.1"
                     /translation="MKAYIFPGQGSQFSGMGKDLFESNSIAKNLFEKANEVLGFKITE
                     VMFAGNDEELKQTRVTQPAIFLHSVILANISESFKPDMVAGHSLGEFSALVASGVLSF
                     EDGLSLVRKRAMAMQRACEINPSTMAAIIGLDDKKVEDICDSIDDIVVPANYNCPGQL
                     VISGSKSGIDRACKLMTEAGARRALPLNVGGAFHSPLMEPARVELEAAILETNFNKPS
                     CPVYQNVNAKHSMKIDEIKINLIAQLTSPVRWTQTIQNMAKEGAKEFIECGPGKVLQG
                     LVRKISPDSLTASI"
     gene            complement(10180..10686)
                     /gene="folK"
                     /locus_tag="VGA21_03440"
     CDS             complement(10180..10686)
                     /gene="folK"
                     /locus_tag="VGA21_03440"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEX9649589.1"
                     /translation="MMDGLFLLLGSNIEDQNYFIEKARMELTRIFGPPIINSSIYKTE
                     PWGNKNQAWFLNQVVIHEFQVLNHQNLLTEIKEIETIVGRKQRDRWGAREVDIDILYW
                     YDEIFQDESLKIPHPQIAGRKFTLVPLVEIAPDKMHPLLGKTQSQLLMECKDTSEVRI
                     QRLSDTGG"
     gene            10769..12517
                     /gene="sppA"
                     /locus_tag="VGA21_03445"
     CDS             10769..12517
                     /gene="sppA"
                     /locus_tag="VGA21_03445"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009583687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HEX9649590.1"
                     /translation="MNFLKTLMAVLVGLILYTGIMFFVLLGIISIAAQEEEVKVEENS
                     VLKLNLNRPILERAIDDPFGIPFFSIPIESTSGLVELKRAISNAKDDPRIKGIYLEAP
                     FVITGFGKLEELRKAIEDFKTSGKFVIAYSDFYTEGGYYISTIADEIYVNPIGIFEFN
                     GLSAEITFYKEMLAKLEVDPVIFRVGDYKSAVEPFLRSNMSEENREQYKSFINSIYAE
                     IINKMAESRRISPQVLRQMSDDLEIRLVLNAKERGLVDDLIYQDQVFDIIRDKIGITD
                     DKEINFISANRYHKSIKSKYSQNKIAVIVASGEIVMGRGEQDMVGAEVFVKEIRQAKD
                     NNRIKAIVLRINSPGGNYIASDILWREIKQAAKIKPVIASLSDYAASGGYYLAMACDT
                     IVSDYSTITGSIGVFRIQFTIQDFLKNKLGITSDRVNTGNYSDMNSVLRPMTDAERQI
                     IQNEVDEVYEVFVSKAAEGRGLSEDSIRKIAGGRVWTGAQALENGLVDLLGNFEDAVK
                     LAAQIAGVENDYSIVYYPEQKSLLEEILYEMGNEAEVKFLKQEMGILYPYVEQLKKLE
                     YLQGIQARMPAELTIY"
     gene            12563..13549
                     /gene="bioB"
                     /locus_tag="VGA21_03450"
     CDS             12563..13549
                     /gene="bioB"
                     /locus_tag="VGA21_03450"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015266361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HEX9649591.1"
                     /translation="MAKIRNDWSRDEISGIFNSPILDLVYRAASVHRKYHEPREVQVC
                     TLLSVKTGGCPEDCAYCPQAARYHTEVKIHKLLPVVEVIESARKAKESGSTRFCMGAA
                     WREVRDNKDFDSVLEMVKGVNSLGMEVCCTLGMLTIEQARKLKDAGLYAYNHNLDTSE
                     EFYGEIITTRNYEDRLNTLSHVRNAKISVCSGGIIGLGENLNDRIGMLHTLATLPKHP
                     ESVPVNALVPVEGTPLEDQEKVSVWEMIRMIATARIIMPRAIVRLSAGRVRMTMEEQA
                     LCFLAGANSIFSGDKLLTTPNPEESADKEMFQILNLKPRKAFKNAPEIPSLM"
     gene            13556..14680
                     /locus_tag="VGA21_03455"
     CDS             13556..14680
                     /locus_tag="VGA21_03455"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HEX9649592.1"
                     /translation="MKQGNVLQDKISRRLAENNLRVLTTSLPGIDFISNDYLGLARSQ
                     KMAGKISEYISKIPYRKNGSTGSRLLSGNFEELEKLEERIADFFYVEAALFLPTGYQA
                     NLALISALGGKDDTLIYDELSHASIKDGARLSLARKYSFRHNDSDDLKRKLSQSSGNK
                     YVIVESIYSMDGDHANLISILNITEENEAFLIVDEAHSTGLTEKNGAGMCVEMDIANR
                     IFARIHTFGKAAGVHGGAICGSNLLRDYIINFSRPFIYSTGPSLHQVISVQASLDELE
                     NADDARAALNDRITFFHDEIKKMNSKLQFLKSNSPIQAIIIPGNDEVKRIAGKILEQG
                     FQVKPILAPTVKRGTERIRICLHSFNTEEEIRKLISVVSG"
     gene            14677..15294
                     /gene="bioD"
                     /locus_tag="VGA21_03460"
     CDS             14677..15294
                     /gene="bioD"
                     /locus_tag="VGA21_03460"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013453161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HEX9649593.1"
                     /translation="MNYFVTAIGTDSGKTVVSAVLCEALKAGYWKPIQAGEPRDSELI
                     KELVSDKTIKIHPEKYLLTQPASPHAAARHDGIKISLSGFSSPWSEGNLIIEGAGGIL
                     VPLNDQDLVIDLVKQFRAKIILVCDLYLGSINHSLLTIEALKSRNLDVKGIVFNGFNR
                     QPFEMESMEIILHKSGFKKLLVMNKESKITPDLIKSWAKQILETL"
     gene            15291..16565
                     /gene="bioA"
                     /locus_tag="VGA21_03465"
     CDS             15291..16565
                     /gene="bioA"
                     /locus_tag="VGA21_03465"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009577742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HEX9649594.1"
                     /translation="MTWSAKDQKYLWHPFSPLQPNSPHLIVTRGEGCYLILEDGRRIL
                     DGISSWWVNLHGHGNKKIAEAIKDQALKLEQVIFAGFTHEPAISLAEKVLFLLPGNQA
                     KVFYSDDGSTSVEVALKIAIQYWENQRINKSRIIALESAYHGDTFGAMAVGARGIFTK
                     PFQSRLFDVDFISTPGLNEGDKVISEFKRAISGNDTAAFIFEPLVQGAGGMLMHEPEI
                     LNEMIAMAQKQDILCIADEVMTGFGRTGKMFASDYLTSKPDFFCLSKGLTGGTLPLGI
                     TTCSDKVASQFPENNFEKTFYHGHSFTANPISCAAALASLEILTSKDCLTSIEQISRL
                     HADFIKKNSFDFLCNLRHRGTILAMDAKTSDVPSYQNPIRKKIYDFFIERNILLRPLG
                     NTIYILPPYIITEIELAGIYAAIREFENTLKQ"
     gene            16618..18174
                     /locus_tag="VGA21_03470"
     CDS             16618..18174
                     /locus_tag="VGA21_03470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649595.1"
                     /translation="MKSIIPFLLTIFISNGIFSQVEDLGQIEFPNSGSAEAQESFIRG
                     VLLLHSFEYEDAAEEFRSAQKTDRDFALAYWGEAMTYNHPIWLQQDKAKALSSLERFE
                     KTSEERLAKTKSEKEKMWLESIEILYGNGTKEENDFEYEDFMKSFSGKFPDDLEIASF
                     YSLSILGTCHGGRDIPSYHRAAAVAEKIYEINPNHPGALHYIIHSYDDPENAVKALDA
                     ANNYSKVAPSASHALHMPTHIFVALGMWEDVVRENIRSANAADARRERKNLGLDARGY
                     HALWWLEYGYLQLGKVNKAREMLTQMHKEALESGSHRTRMHFSVMKAAFLVETDNWSE
                     DVLSMKVDLAGMNNWVKVLNGFLEGYGTIRNNKIERAKSILDDLKKINTGISSFSEYD
                     LNEKEAAIMVSILESYYLFESGKTTEGINLLKKAREAEESLSYSFGPPAIPKPSHEIL
                     GEIYLHERNFELAESNFRISLERAPQRVMSLKGLYAALKGLGKSGQAEEVLAEIKKIR
                     KSADQDVYPN"
     gene            complement(18200..20215)
                     /locus_tag="VGA21_03475"
     CDS             complement(18200..20215)
                     /locus_tag="VGA21_03475"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008623283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEX9649596.1"
                     /translation="MKNLSVLLLIISFSAKGQISDKLEPIDVFDLEYVSDPQISPDGN
                     RIIYTRNFKDIMSDRNLSNLWVINFDGTNNRPITTGIQNDYNPIWSGDGRQILYMSNK
                     EGSSQLYLRWLDTGAESKLTNLQNSPGGISWSRDNKFIAFSMFVEEPEKPFVKMPAKP
                     DGAKWNDAPKYIDDISYRGDGRGYLKQGYNQLFTLPADGGTPRQITSGPYNFNNPEWT
                     ADGKNLICTSNMHENRELEEARNSEIYSVSVAGGKITPLTTRKGPDGGPEISPDGKLI
                     AYSGFDDRYQGYQLTQLYVMSPEGTNSRLISKGFDRDIQEYYWSGDNKGLYIAYDDVG
                     NTKIAYMDLNGKVSELTNYVGGLSLGRPYTAGTFSVTATGRYAYTLGGPDHPSDLGAG
                     ERNNVNRLTRLNDDLFAYKKLGKVEELWWESSFDKKRIHGWIVTPPDFDPSKKYPLIL
                     EIHGGPFAAYSPFFAAEIQLFAAAGYVVLYTNPRGSSSYGEEFGNLIHHDYPNHDYED
                     LMSGVDAAVAKGYVDETQLYVTGGSGGGVLTAWIVGHTNRFRAAVVAKPVINWYSFVL
                     YSDGIPFFYKYWFPGTPWENLEQYMRRSPISYVGNVTTPTMLLTGESDFRTPIAESEQ
                     FYAALKIQKVETAMVRIPGASHGIASRPSNLIAKVQAILGWFEKYKE"
     gene            complement(20301..20813)
                     /locus_tag="VGA21_03480"
     CDS             complement(20301..20813)
                     /locus_tag="VGA21_03480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649597.1"
                     /translation="MERNKFLSLAGAGALGISFAPVISLARQNQRPEPYKQELTLDFV
                     AAGHTDLTKVKEMLGEYPNLLNCTWDWGGGDFETAIGGAGHMGNIEIAEYLIGQGART
                     NIFVLTMLGKTELVRPILEEYPSLINAKGPHGLTMLHHAEKGGEKAKELYDYLVSKGL
                     TTKKVDLYKK"
     gene            complement(20891..22054)
                     /locus_tag="VGA21_03485"
     CDS             complement(20891..22054)
                     /locus_tag="VGA21_03485"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018620923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEX9649598.1"
                     /translation="MIFNVETRSKSLLTELCCSRCSATYSPSELYGLSPCCKATLINK
                     YELVLSREVLKDKPWTMWRYQELLPVLNPENIITMGEGGTPIFSLNSKIDGLEKQILV
                     KDEALNPSGSFKARGMSVAISKAKEFGIKKCITPSAGNAGVAMSAYCARANIQSTVVF
                     PAITPKIYIDECKAYGAEVILVDGLISECGKKVMEINSMGEYFDVSTLKEPYRIEGKK
                     TMGYEIAEAMNWKLPDAIVYPTGGGTGLIGIWKAFRELQQLGWIEGLLPKMIAVQSEI
                     CSPVVDAWENKAINPSRYQASIALGLNVPNPFGLELIMQCLKESGGAAVAVSENEIRL
                     ALTGLTKKEGILVSPEGASAFAGLVKLVESGFITPSAQILILNTGSGMKHLVN"
     gene            complement(22114..23016)
                     /locus_tag="VGA21_03490"
     CDS             complement(22114..23016)
                     /locus_tag="VGA21_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007659329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX9649599.1"
                     /translation="MISFRHQVFVEVARQLSFSRASQNLFITQPAVSNHIKALEDYYK
                     TALFFRNKNSIGLTPAGAILYKNLAVMLDKQKEIELELSKVSKNFEPQIELLLGASTT
                     ISLYVIPPVLSLYLQKNPGFRVTLLNRNTEKIIEALANNEIDLGIIESHHKESSFDYS
                     QFMTDEVIPVCSKKSFLTKKSNISLSELKSTPIALRERGSGTLEVVKSELEKKGISLS
                     NLEVIVRLGGTEGLKNFILADQCLGFLPRRSVMKELKSGELVEIKIQGLSISRKFQFI
                     QRKGQRSMPVYKEFVRFARSYYNK"
     gene            23084..24982
                     /gene="nagB"
                     /locus_tag="VGA21_03495"
     CDS             23084..24982
                     /gene="nagB"
                     /locus_tag="VGA21_03495"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020526869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006046 - N-acetylglucosamine catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HEX9649600.1"
                     /translation="MKNTRFEHLPTKIFNSNKDASRYAALEISDLIRKKAANSEICVL
                     GLATGSTPISIYNELVRLHKEEKLSFANVITFNLDEYYPMQPHALQSYVRFMHEYLFN
                     QIDINKNNIHIPDGTLSKSRVQSYCKAYEKKIAEYGGIDLQILGIGRTGHIGFNEPGS
                     DINSETRLITLDSITKTDAASDFFSEENVPYQAITMGVGTIMKSGRILLIAFGEGKAG
                     IIKKAVEGKVDEQVPASYLQKHHKAEVVLDVAAADELVRIKSPWLVQTCKWDDKLIKK
                     AVCWLSLKLDKPILKLTDRDYIDNGMDNLLIERGSAYKINIDIFNKLQHTITGWPGGK
                     PNVDDTYRPERAAPSKKRILIFSPHPDDDVISMGGTFIRLFEHGHDIHVAYQTSGNIA
                     VFDDDVIRFADFSNEFNKLFKVKDPQLTKLFQRVSNFINKKKPGEIDNEEVRKIKTLI
                     RRSEARSACRYIGLDINNAHFLDLPFYETGTVKKKPLGEKDITITVNLLRQLKPHQIY
                     AAGDLSDPHGTHRVCLEAIIGALEACKKDSWLKNCYVWLYRGAWQEWGIHDIEMAVPL
                     SPGELLKKRKSIFKHQSQKDSALFPGPDEREFWERAEARNRATADIYNKLGLPEYEAI
                     EGFKRYYF"
     gene            25050..25487
                     /locus_tag="VGA21_03500"
     CDS             25050..25487
                     /locus_tag="VGA21_03500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649601.1"
                     /translation="MKFSELISMFRAGKAGAKSHMKNLIEMAAADGNFDDVEYNLLKQ
                     IAKKHKVSEKELKKIKDNPQNVAFEVPSDPKEKFDQLYDLVNMMVIDNYVDREEVNLC
                     LIFAGKFGYKKERIVELVDSIKDNIKNNQDQEATRKRLDWLLN"
     gene            complement(25480..26358)
                     /locus_tag="VGA21_03505"
     CDS             complement(25480..26358)
                     /locus_tag="VGA21_03505"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009581830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HEX9649602.1"
                     /translation="MEKEKLSLQQVLDMGAPEVIRYAVPVMIMLVLAEWGISVYQKRD
                     LYDKKDLFAAATIGIGNLIVSAIIKTALFASVLFFLNLVPWSIPPVWWSYILCLVAID
                     FCRYWAHRIAHENRFWWATHVTHHNSEKYNWSVSFRLSWVQHIKFIFFVPVALMGFHP
                     VIFFICHQIEVLYQFWIHTEYIRKLPRPIEYIFTTPSHHRVHHATNEKYMDKNYGSTL
                     IIWDRMFGTFQPEEEQAIYGITKPVGTYNPITLVFHEWRDIYKDVSKARNLSEARKYI
                     FGRPSELAKESEKATN"
     gene            complement(26479..26658)
                     /locus_tag="VGA21_03510"
     CDS             complement(26479..26658)
                     /locus_tag="VGA21_03510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649603.1"
                     /translation="MILLSRISQCNSLARVITEKQFILDFIPEVYFRNEKYYSEGNLV
                     CRNTNTPARLWLWRK"
     gene            26936..27559
                     /locus_tag="VGA21_03515"
     CDS             26936..27559
                     /locus_tag="VGA21_03515"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HEX9649604.1"
                     /translation="MKKLFTILLTGLMIVGMTIQSHSQGFQFGIKGLAQVSSIHGLDD
                     TGVSDAKSKITTGIGLFATIKLSKIGVQPELQLLKQGSKFEDSGGTEYNQKMTYFKVP
                     VMINLYIVKQFHLEAGPYFGVLLAAEQKLTSDPLNLGTGPIDNKDNFKSTDVGIAAGV
                     GADIKRLNLSFRYMLGLTDINDSSGTTEKLKNGIFQFGIGISIINKD"
     gene            27715..28323
                     /locus_tag="VGA21_03520"
     CDS             27715..28323
                     /locus_tag="VGA21_03520"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX9649605.1"
                     /translation="MVKDETTEQSILKSARETFKTRGFSGAKMAEIAKEAGINKALLH
                     YYFRSKQNLFDTVFAEAVSVLIPKLIALLQSDLPLDVKIYKIVDYYYLTLSKNRDLPI
                     FVLSEIRTNPGFMLHMLKENGMDHLVLDKQIEQEISKGNMIPVSTPEFFSNVMGLILF
                     PFIVEPLIKGLFQMSEIDFQNFLEKRRHELPSQILKLFKLKS"
     gene            28320..29591
                     /locus_tag="VGA21_03525"
     CDS             28320..29591
                     /locus_tag="VGA21_03525"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX9649606.1"
                     /translation="MTSIKSKLILLAVAAVLWLDLNGQDLSLQDCYHAARNFSPVKKQ
                     QPNIAQISSLVMESLKKEKLPQIFANGQYTYQSDVVSFPDFPGINRPDIPKNQYRISL
                     DINQSLFAGNRINKMVETEKARLQVDQQELEVKLSGIKSSVNELFFAALSLQEKEKLY
                     DIIIGDLKTRISLIQSLIRNGVLLPGNEKSLLIEIKRAEQEKTGLYFQKSGVLRMLEE
                     KTGLEISSERNLVVPEFQFVQSNNLKREEKLLFKYSHATLQEQMQLTNLSRYPKLSAF
                     GSLGIGNPNPVNFFETGTSEFYLVGLKLSWNIWDWNNSKRAKQILQLQQQNLNEEERD
                     FDLNVFTQLAGANTNLEKLRTLLISDKEILELQKEITEEAFSRFREGVITPSEYSTEL
                     NRQIQAQLKLKMREIEISQAIVNILTLTGHI"
     gene            29607..30500
                     /locus_tag="VGA21_03530"
     CDS             29607..30500
                     /locus_tag="VGA21_03530"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEX9649607.1"
                     /translation="MKRNIKIVLFLLPLASCNYNAELADAYGNFEADETVISAETAGK
                     LLYLNVQEGTEIPSGLDVALVDTTQLFLKKEQLKAGINAIISKVQPVQSQINVLIEKR
                     RNIAREKNRIEKLLADSAATRQQWDDISGQLELVDREMTATRERLESANKGILSEIKS
                     LEVQIAQINDQIVKSLVLNPAQGTVLQKFAEPGEVTAFGMPLYKVALLSQLYLRAYIS
                     GNQLATLSMGQVVSVGVDDGEGGIKNYPGRITWISSKAEFTPKVIQTREERVNLVYAI
                     KILVENDGLLKIGMPGEVYFN"
     gene            30506..31423
                     /locus_tag="VGA21_03535"
     CDS             30506..31423
                     /locus_tag="VGA21_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015030403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9649608.1"
                     /translation="MVIRVQEISKHFKTVQALKNISFQVAEGEVFGLIGPDGAGKTTL
                     IRIMATLLKQDAGNITLAGYNNIEDYEQVRNLIGYVPGKFSLYQDLTVEENLNLFATI
                     FGTTLQENYYLIKDIYQMLEPFKKRRAGNLSGGMKQKLALSCALIHKPKILLLDEPTT
                     GVDPVSRKEFWEMLGRLKNEGITILVTTPYMDEAAKCDRVGLIQTGNILSIDTPQNII
                     TGFPLKIYSVRSLKRVELLHHLRDMPNAHSANLFGDSIHYIQKMPEPDELTLEAALKQ
                     AGYLDAKVERIQPVIEDCFIELMKSQIAA"
     gene            31423..32139
                     /locus_tag="VGA21_03540"
     CDS             31423..32139
                     /locus_tag="VGA21_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020529923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9649609.1"
                     /translation="MVIEVENLVKKFGDFTAVDNISFEVKEGEIFGFLGANGAGKTTA
                     IKMLCGLMAPTSGRGVVAGFDIKTQREKVKRRIGYMSQKFSLYDDLSSYENIMFYGGI
                     YGIRRKELKKKAERLIRKLGLESVENKLLKSLPPGWKQKLAFSVALLHEPSVIFLDEP
                     TGGVDPVTRRQFWELIYETATEGKTIFVTTHYMDEAEYCDRVSIMVNGRIDAMASPAE
                     LKQNFEVTSMEDVFLKLARS"
     gene            32136..33239
                     /locus_tag="VGA21_03545"
     CDS             32136..33239
                     /locus_tag="VGA21_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019944462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9649610.1"
                     /translation="MKRFAGFFKKELIHIIRDPRTLIILFGMPFVQILLFGYAITNEV
                     KNARIAILDNSKDEVSFKLIDKILSSGYFTMSEYLQTSAEIDHAFKQGEVKLAVVIPE
                     RLSEKLKRNELVQIQLISDATDPNTGNTLINYTSAIIRDFEFSRSENLRPVLINPEIK
                     MRYNPGLKSVYLFIPGIISVILMLVSTMMTSISLTREKELGTMEVLLASPMKPAQVII
                     SKVIPYLFLAFGNALLILALGYIVFDVPVKGSLTLLLFESLLFIAVALSLGILISTKT
                     DSQQVALMISLFALMLPTIILSGFIFPVENMPVILQIISNIIPAKWFIVIVKGIMLKG
                     IGIAELWKETLILVGFIFVLIIASVKNYKVRLE"
     gene            33236..34348
                     /locus_tag="VGA21_03550"
     CDS             33236..34348
                     /locus_tag="VGA21_03550"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9649611.1"
                     /translation="MRVLLIIIQKEFKQIFRNRGMLPIIFVLPIIQLLILANAATFDI
                     KNIKIRIVDKDLSSESRRLVSKFEGSPYFLVEKWSLSSFNNEYDLDVGTVDLILEIPN
                     HFGRDLYETGFSSVQLLVNAIDGIKAGLGTNYAQSVMVDFLGEFGATAPGSKIAKPIE
                     IIPANWYNEELNYKEFMVPGILVLLVTMIGAFLTSMNIVREKEIGTIEQLNVTPIHKT
                     QFILGKLAPFWVLGLFVFGFGLLVGKILYDIPIEGSLWVLFAFAGVYLFTVLGIGLFV
                     STVTNTQQQAMFISWFFLVIFILMSGLFTAIENMPMWAQKITYFNPVRYFMEVVRLVL
                     LKGAGFKEVQRHFVIMAVFSTAVISLAVLRYRKSSG"
     gene            34406..35557
                     /locus_tag="VGA21_03555"
     CDS             34406..35557
                     /locus_tag="VGA21_03555"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEX9649612.1"
                     /translation="MSIKNTRREWLKSAALLAAGLSTANFTLSNPTKTPVYNPYTGEW
                     MLNLPDQGLRARLNYNENPYGPSEKALQALRDALGESSRYAFSMADQFKEMIAEKEGV
                     SADHIFLSAGSLEFLALTALAHGLEGGNIISAYPTFQSLMDTAAAFDCEWKRIPVDSN
                     YKHDLGRMEEAVNSNTKLMYICNPNNPTGTFLDTAELAEFCKRVSNRVTVFVDEAYTE
                     FHPDPESITVKHLVKEGHNVIIAKTFSKIHGFAGLRVGYAIAQPAKIRKIEAYRLQMT
                     TMTGPSIAAAMASYQDQEFMNFCRKKNTMAREQTYTILKDQGYEYVPSHTSFMIFRIN
                     TEPKSFLKGMRDQGVGVRSWTFHNKDWCRVSMGTVEEMEIFGNALKMIS"
     gene            complement(35708..36193)
                     /locus_tag="VGA21_03560"
     CDS             complement(35708..36193)
                     /locus_tag="VGA21_03560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649613.1"
                     /translation="MKTRILTLIALFILPILGHSQKKLTDKDITGTWKLVIVMEEDNE
                     DNEDDEDYIDREVDESFLGHVISSGVKALVANIMEEIDIRFEFLKNGRLEIYAMGERE
                     DENDNRWFINSKGELIITEEGDQYDEDEIWLMKDDKLVAFKRRGTKLKEKNVYLERID
                     K"
     gene            complement(36299..37678)
                     /locus_tag="VGA21_03565"
     CDS             complement(36299..37678)
                     /locus_tag="VGA21_03565"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX9649614.1"
                     /translation="MSNSEYIHSLILYLTQGAGALILAIILYRFYISYRHKYLKSWCL
                     SWIGFGIYLIGIAISYSLIEVIPPDNGIRIFITLIGLFFGYFQLVFLTKGTYEVATKG
                     AVKNRIVVLSLILVLVASLVFVFVNIQDPNALGRILTRAGFRSLISGVSFMVAAFMIY
                     RSTMIHRGLGVKLVFWMFLIYGLQQFHYAIAIIMGYMGNHTLFGFTIYLGVADLLIQS
                     LMGVGMIVWLLEDESRELKKANKDLDSLVYSTSHDLRAPLASVLGLLNVARLEKDQSK
                     VSEYFGMIEERINKLDSIISDIMVFSQNNKGALRIRKINIRTAINETVEDLKFSEGFN
                     SIKVEVDIKPDFEFQTDQERFKIILNNLISNSIKYRGRNEPFIRISAHIARENLILEI
                     EDNGIGIEEAHIGRIFEMFYRASENTGGSGLGLYIVQEAILRLGGTIKVTSVPMKGTK
                     FTLELSALK"
     gene            37807..38658
                     /locus_tag="VGA21_03570"
     CDS             37807..38658
                     /locus_tag="VGA21_03570"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9649615.1"
                     /translation="MGPLPEKNPLKARRISNSKHTAWVRDRRWKVAKVEDFEIENGLR
                     MRVYKPEMPTNGKVICYFHGGGFVIGGLDSHDNICRRLCQSARVNLYSIDYRQGPEFK
                     FPAANEDAIAAIDWVRKQVVDGVATTDLLVAGDSAGGNLAAFASLHYPDLIHKQILIY
                     PVLDFIKPKPYHQIKYSFFNSENDASWYAKHYLNDEKEKLDSRLSLLTRKDLAKSPET
                     MIVIAEHDILKFEAIEFAKKLISLGIKVDVKKYAGMVHGFLNFPRWASKAKTTFEDIA
                     EFILKKD"
     gene            38684..39358
                     /locus_tag="VGA21_03575"
     CDS             38684..39358
                     /locus_tag="VGA21_03575"
                     /inference="COORDINATES: protein motif:HMM:NF015331.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX9649616.1"
                     /translation="MIATGLTFATKRNFFLLISTIALGSAYGQDVPWEFAQDKEGIEV
                     YTRQPGGSKFKEFKGITEIKLSLHSAIALLTDVNTMPEWVSDCESTKLIEKKENKVIY
                     HMVIGVPFPFDDRDMVQELTFIYDKETRNMEINLGQNDEIMDPLRGVVRMPVSVGKWT
                     FEHLGKEKYRVTFEYLSDPGGALPAWLVNPFLIRMPYKTLLNFKERVNYPQYKSAYFD
                     WLGATK"
BASE COUNT        12195 a         7529 c         8125 g        11611 t
ORIGIN      
        1 atttttccat tatagtcatc cgcctttgtt gaaaaggctg gaggccatca aatcgcaaga
       61 atgagcaaga tagtgtcttc gtgcccttgt ggtaggattt aattaaccgc caagtcacaa
      121 agtcaccaaa tattcgagga gtaagttgaa agcagaaatt agtgtcctgg tgtcccttgt
      181 ggcaaagatt catttagtca cgaagaacac aaagtcacca aagtattcca gaagtaagtt
      241 gaaagcaaaa aactagtgtc ttagtgccct tgtggcacgg atttaattaa ccacgaaggc
      301 acaaagtcac caagtattcc aggagtaagt tgaaagcaga aaagtagtgg cttagtgccc
      361 ttgtggcaca gatttaatta accacgaatg cacaaagtca ccaaagtatt ccagaagtaa
      421 gttgaaagca aaaaagtagt ggcttagtgc ccttgtggca aggacttaat tagccacgaa
      481 gaacacaaag tcaccaagta ttcggagata aattagtgtc ttagtgccct tgtggcacgg
      541 attaatccaa aaaaataata ataaagagta ttagtaatac aataattcca tatttatatt
      601 tgaaattgaa tttatcacgt aattaatatg aaaaaatcaa ttaacatact tatcctgctt
      661 gttctcattt cctgccaggt tcaggaaaaa ctcgaaacca accccggcac cataaaattc
      721 tccatgcaat ccgttgtccg gaatgaaggc ggaagaatta cagaaactcc aactccggct
      781 tcagttttaa tttccattga agatgaatcc ggaaacacgg ttgaggacaa caagaaacta
      841 acactatatt ctttgggtca gggttttgtg agtgaatcat tgcaattgat tcccggcagt
      901 tataagctta ctcagttctt gattctgaat gaggatgatg gagtgattta tgccactccg
      961 caggaaggct cggaattagc ggaatttgta aacgaccctc ttcctataga attcaccatt
     1021 agtgaagatg aaaatacgct ggtaacgcca caggttttgg cggtacatcc ggaagataca
     1081 cccgagagtt ttggcttcgc gagttttggg tttgaagtcg tggcaccaag gctaaaaaaa
     1141 gtcatatata catctatatt cggtatccat ataagacatt ttagttataa tggagaatta
     1201 ctagctgaaa tagccgagaa aggatgtcaa acatgtagtt caggtaattt tgaatattat
     1261 ggttcagtga atttcgaata ttccccggat aagaaattga taaaaagaac ctatgttaat
     1321 aaatatggtg tggtcacccc atatttcata aatgagtatt caggtggatt attagtaaag
     1381 cggaagattt tcggtccctc ttatgagtat gagcagcaat acacatacga tagcaacact
     1441 catttaatat caatgactga aagtacaggc aggttgcatg aatttgaatt gagcctggag
     1501 tcagataaat taatagataa attattcttg aactttcaaa ttccagaaag tctgcgattt
     1561 attacagagt tttcttttga tgggaaaaga gcaactgcat ttccaattac aggctcagaa
     1621 gaaataattg acatttatgg atatcctatt ggacagaatg tgatcggtat caggcagaaa
     1681 gatttaaata caataactgg agaagtcaat tacgaatgtt atgcatcaat tcaatatgaa
     1741 tataactcat taggcttgcc tgtgaaagca accgtttcaa atcccgatgg ttgcattttt
     1801 gaaatagaag atggtaccgt gattgagttt ttctatgagt agcaatttca aatcagcaat
     1861 atgaaaaggt taaaagtatt tcttttaata actcttgcct cctgccaggt tcaggaaaaa
     1921 ctggaaaaca cctgcattag taaaattctt ttagaataat caaaatacct tagtgccttt
     1981 gtgtcttggt ggctgagaat tgaaatatga ccttcgaaaa attatcaaag tgcgaggaag
     2041 caataggtaa agcaatcgta acgagcgcat tcaaagttca ttcagagctt gggccaggtc
     2101 tgctagagaa aatttatgag gtctgtcttg ctcatgaatt gagaaaggca gaatgtgagg
     2161 taacccgtca attagaaatt cctattcatt atgatggaat tacatttgat gagggactaa
     2221 ggctggatat ccttgttgcg aaaaaagtaa tagtcgaaat aaaggcagtg gatcaggtca
     2281 atcctgtgtg gcaagcgcaa gtattaagtc atctgaaact tacaggtctc cgattgggtt
     2341 atctaattaa ctttaatgtt ccattgatca aggatgggat aaaaaggatt atcctataac
     2401 cttagtgtcc tggtgccctt gtggcgagga tttaattaac cattaataac acaaagtcac
     2461 taagtattcc cgtagaaaat tgaaagcaaa tactagtgtc ttcgtgccct tgtggagagg
     2521 atttaattaa ccacgaaggc gcaaagtcac caggtattcc agaagtaagc tgaaatgctg
     2581 aaatcagaaa actagtgtcc tggtccttgt ggcaaggaat ttctaatcca aaaaatcaat
     2641 gtcagcatgc agcggaaacc gcatcagtcc ttctaatgct ctttcaacac tcagcttacc
     2701 aaacaaaacc tggtaaaggg cttccgtaat gggagctcta accttcaaat aatcagcggc
     2761 aaatttcata atatgaactg tgtttactcc ctcagcgact tcttccatag ttgatagaat
     2821 ctgctgaagc gtttcacccc gcgcaagcct gtaaccaacg gtaaagttcc tgctcagatt
     2881 actggcacaa gtggccacca aatccccgat tcctgcaata ccaagaaagg gatgtacctc
     2941 accacctaac gcttttccaa ggtgaatcat ttctaccatg ccccggctaa ccagcaatgc
     3001 ccgggcattt tctcccaagc caagaccaga taaagcccct gaagctatag caataatatt
     3061 tttcaagaca ccagccagtt caataccccg aagatcttca tttccataca ccctgaacct
     3121 atcattacgc agcaatcgtt ggccttcatg aataacttct tcaaagtggc tggctaccac
     3181 cgtcgccgca ggttgcttgt cggcaagttc acgggcaaga ttcggtccag ccaggcaacc
     3241 cacgcgtaca acagggctct catccaggat aacctgtgac atggtcttaa tcttttccac
     3301 atcaaatttt cttctatttt gaatctcttt ttcggtcatc tggatatcca gtccctttgt
     3361 tccgtgaatt agtatatgat aagggtgaag caaaggaccc agcccttgca tcatttctct
     3421 aaatcctgcg gaggaaacta ttgggaaaat aacactgcag gtagtggcta tttctgcaat
     3481 atcactggta agccgaacct gttcgttaag ctgttggccc gcacttactc ttgctttccg
     3541 aatttcgtcc acaccttcag catttctccc atagagtaat acatctgaat tagcagcaag
     3601 aatattggat attgcagtgc cgaaactgcc cgtacctaca acacctacag tcctatttaa
     3661 tcttcttttc aaggtcataa ccctttagtc ggttgtgata ataataaagc cgggctaaat
     3721 ccattgttat gatattacct tttttgttct tctttagagg tcgcaaagtg tgatacaatc
     3781 caaggttact cagcccatga ttaataacct catctacatc gccttccaga tgtggagcag
     3841 cacccactat tccctttttt ttcattttaa aaatcttctt tcgtaaccgc tttacatttt
     3901 gcctgaattc ttcataaggt atttcaattt catcttcagg gattcgaagc aaattaaaca
     3961 aatcaaactg tggatattta tttttgatca tttcgaatgc tacaaaggca attatgtgac
     4021 ttgagaagac tctgtttgaa atatggaatt ccgaaacaat tgccttggca agcatccggg
     4081 tatattcctc ttccctttgt ttgttttcag taactcttcc ttcatagaca aaatattccc
     4141 ttgaatcaat tattttacct cttttgtccg tgctattacc ttcatcatct acatagtttc
     4201 cgagaacatc cactccatga ccaatggaaa ctgaaatatc tgatcctttg gtgaaaaatt
     4261 taatcatgaa cttgagaata ttatatgagt tggaaagatt gtcattctca acataatatt
     4321 tctcctgccc gcgaagtcga aggtaatcgt taattaacaa tggcgcttca agcacaaagt
     4381 gataattaat tactaccggg aaaatgaaaa tttttcttgc atcaggtcct ggattgtcct
     4441 ggtagatttc acgctgggct tcaattgttg ttcccagcaa acccagtttt agggaatctt
     4501 caatctgtcc tgaccttgat cgggttccac ctgggaaaaa taaactatga gcccctttct
     4561 ttacggcaag acttgaataa gccttgagag tttccaggta gggtaaattc tttttcctcc
     4621 tgtccacttt ataagcgcca aggctattca taaaataagc aaatatgcca atattgaaca
     4681 ggtttaagcc ggcgccgtaa ataaatggcg gtagcccaag ttcatgaata acccagccaa
     4741 taagtacaga atcaagatta gagaaatgcg tgggcaacat gatcacggtt ccttttttgg
     4801 caagttttct gatctgctca gtgtggccta caatgttaat tttatcccgc agtgtcaaat
     4861 ccctgcgaaa tatggcgccg aacttcttga ttcttgccgc atttaacagg cggcggaaac
     4921 caaaagttgc tattgctctt gctacccgat acgatgatgc tttgaagttt cccgagattt
     4981 cattagcgta cctgctgaca atggactgaa ggatttcttc ttcaacttta ttgtctctgc
     5041 cattggtagt agatgatagt tccaataact tctgtttaac tccctcccaa aaaacagctt
     5101 catctggcgg atcaactttc catggatatc tcttgattct gagcttttct ctgtaaagtg
     5161 tagtttccag ttcttcaata agtgattcct ttgttggagt ttgtttcttg attcttttta
     5221 tagcaagttc tgtaacttcc cgaataaaag tcttcttgtt tctggacaat tgaaagacag
     5281 accaatcccg gggtcctgaa tggattggct cgaaaccctt cttgcgagtc tttccaggct
     5341 tttttataat tgtattatcc attattgaat gaaggggttt atcaacttag agccactaat
     5401 ttattaaata agggctaacc ttagcctatt gtatttttat tagtttatgt tattttatac
     5461 aaaccgctaa gtgttgcgtt actccctcta ttgatttatc tcactattat agtattttat
     5521 gaaatcctct agattctttc cggttttccg aataaaataa tgatgagttc cgccggtagt
     5581 ttctatagaa atggcagccg agccaccgaa gccataaagc aaaagaaata accccgcgat
     5641 cattagtgtc atcatcaaga agggattgga aatgtttccc aaaaaggcaa gacttgaaag
     5701 tggaaaaata atgccacctg tgacaatagg accaagtagt tttttattac caagtgaaat
     5761 tctcctaatc tggttaaccg gcgtaggatg ttttcttcct ttcaagataa ttattaacat
     5821 atcttctgta aaatacagac agttactctg atcgccattt tttttgagga aacatatggc
     5881 tttaaccgat gggtcataca aaggattatt attcaatctt tttgaatggt aatgatggag
     5941 ctaatttcct aaatttttcc atgatgagca tgttttattg tatctgttaa aggttacttt
     6001 tagcgagttt gcacgatatt tgttcaaaca atggcctgcg gaggcaatta atgattttgg
     6061 gcttgagaca attgttttct tttctgaaaa aattcctaat cccggtactc gcaatttcaa
     6121 tatttgtaac tgaagcccgg gcaacccatt taagggcagg ggagattact gtccaaagaa
     6181 tttcctgtca ggagcttact tttcgaatta caataacagt atttactgat attggtcctc
     6241 aggttacagc aaaatttgga cagggtgagt tggattttgg tgacggcacc gttgagttaa
     6301 ctccgcgtca ggaaaaccct ctggatttgg gaaacgatgt tggctacaat cagtacgtaa
     6361 cggagcacac ttaccaaggt aatggtattt atacgataac atataccgaa ccgaaccgta
     6421 atgatgaaat attgaatatg agcaattcag tgaatacaaa gtttcatctc gaaaccgtta
     6481 tttttctgga tcctttttta gggtgtaata atactcctgt cctgttagtg ccccccatag
     6541 acagaggttg tactcagaat gctttctacc ataatccggg agcttttgat tttgaaggag
     6601 atagcctttc ctatgaattt gttattccaa agtcagaaaa aggaattaac gtactggatt
     6661 atgttttccc gaatcatgct tcattcaatg gaatggatga agatgggatt ggtcccgcca
     6721 ctttgactct taatccttcc accggagaaa tagtttggaa ttctcccgga caacctggtg
     6781 aatataatat cgcattcaag gttacagaat ggagaattat tgatggtatc gcatttaaac
     6841 tgggtcatgt aaccagggat atgcaaataa ttgtagagga ttgcgataat gagagacctg
     6901 aaatagtagt tccggaagat gtttgcgtta ttgccggcga aatagtgcaa ggtgttgcca
     6961 ttggttctga tcctgatggc gatcctgtta aagttgaggc ctttggaggg ccttttgaag
     7021 tgacccccgc tgccgtaacc atacctgcaa atcctggttt tgttccaacc cctacccagt
     7081 tagtttttga atggcaaacc gattgtaatc acatccgaat tcagccctac caagttgagt
     7141 ttaaagtgtc tgataatcct ccgaccggtc caactctggt ggaattcggt acctggaaca
     7201 tcaccgttgt gggcccgcct ccggtattac tatgtgcaaa tccggaactg atgaccggaa
     7261 gaaatatacg actggattgg gaggcttata aatgtgataa tgcagaaact attcaaattt
     7321 ggagacgagt tgatagtttt gactttgatc ctggtgaatg tgttaccggc atgcctttat
     7381 atgcagggta tgaattgata aagatagttc cggctactca gaactttcat atggatttga
     7441 acctagatgt gggtgcttca tattgttacc ggttagtagc tgtatttcca gcgccgggtg
     7501 gtgggcttag tgttgtaagt aatgaagttt gtgcagatcc aatcctggcc gatgcgccag
     7561 tcattacgca cgtaacggta gaggttacag atgaaattga cggacagatt aatgtaagtt
     7621 ggacggcccc atttcagctt gacccaatcc aatttcctcc accatatcga tatgatgttt
     7681 accgggcagc cggattgatc ggggagacag agcttgtcct tgtaggttca acaagtgaca
     7741 ccactataac tgattcgggg ataaacaccg tagaattacc ctataattac agaatatatc
     7801 tttttgatgt aaatgatagc cccgttgata cctccgcggt tgcttcttcc gttcgtttga
     7861 ctccacaacc gctaattaag gagatacagc ttaactggat agcttcggtt ccatggtcaa
     7921 atcaggtgca ggatttccct taccattaca tttacagaga caatgctgac tcaggcgatc
     7981 ctgctaaact ccagctcatt gattcagtca atgttcttct aaatggattt acatatagtg
     8041 acacaggatt taatggagtt gaattaaagg aaaccacaga gtattgttac tatatagaaa
     8101 ccatgggatc atacgggaat ccactcatcg ccgcaccaca aagaaacctg agtcagatta
     8161 tttgtacgca gcccagcgat actattccac cctgtgctcc tcttgtgagc atagatacca
     8221 tcgattgtga aaattttaaa ttaactgaac tgtgtaattt caacacctat tcaaatacaa
     8281 tttcgtggaa tgaacctcag gaatgtgcag atgacatccg aagctacaat atctacttta
     8341 gcagaaccgg ccaggaaggt tcatttaatt taattaataa cattacagag acaaattttg
     8401 tgcatgataa tattccttcc tttgccggat gttacctggt cacatcagtg gacaggtcag
     8461 ggaatgaatc tgatttcagt gaaatggttt gtaatgataa ttgtccgaat tacgttttgc
     8521 ccaatgtttt taccccaaat ggggatggat ataacgatgt attcggagct tttaatcaaa
     8581 ctaacttgcc tagtgaagat gagccgggta gcatgttgcc tgatctttct aaatgccctc
     8641 gcttcgtgag agcagtgaat tttatggttt ataacagatg gggcaaagaa gtctttaaag
     8701 caacatctgc aggtgaaaat agtttgttta tcaattggaa tggaaagaat agtgacggat
     8761 ccgatgcctc ggccggaact tattactatg tagctgaagt tacttacgat gttctgaatc
     8821 ccagggaatc agtaaggatt ctgaaaggat ggatccagct ggttcgttaa taaatgaata
     8881 agccagttat actatttgac ggtgtatgta atttttgtaa tgggtcggta aactttatta
     8941 tcaaaagaga tcctaaagcg aagtttagtt tttgcccttt acaatcagac gaggcaatta
     9001 atctaatgat ccccttcaac atggatcccc gtgcatctga tacaattatt cttctccgga
     9061 ataataaact ttacatcaaa agccgggcgg ctctcgaaat aataaaagaa cttaccggat
     9121 tgtggcctct tttgtatgga ttgattatag ttccgccttt tatccgcaat ttcttttatt
     9181 caattttagc caaaaatcgc tataaatggt ttggaaagat ggatgcatgt atggtgccct
     9241 ccggcgaaat caggaatcga ttcatttctt aattcgttgg gaagaaaaat aagcctattt
     9301 ttgatgcctt acaaaatcat tttcatgaag gcgtatatat ttccgggtca gggttctcaa
     9361 ttttcaggaa tgggaaaaga tttatttgaa tcaaattcaa tagcaaagaa tctttttgaa
     9421 aaagcaaatg aggtgttagg atttaaaatc actgaagtga tgtttgccgg taacgatgag
     9481 gagttgaaac aaaccagggt gacgcagccg gcaattttcc tgcactccgt tattttggct
     9541 aatatttcgg aatcatttaa acctgatatg gttgcaggtc attcattggg cgagttttcg
     9601 gcacttgttg ccagcggtgt actttcattt gaagatggtt tgagcctggt gcgcaaacgg
     9661 gcaatggcca tgcaaagggc ctgtgaaatc aatccttcaa caatggcagc aatcatagga
     9721 cttgatgata agaaagttga agatatctgc gactccatcg acgacatagt tgtacccgca
     9781 aattataatt gtccgggaca attggttatt tcaggatcaa aatccggaat tgacagagca
     9841 tgcaaactta tgacagaagc cggagcccga agagcattac cgctcaatgt aggtggcgcc
     9901 ttccactcac ctttaatgga gccggcaaga gttgagttgg aagctgcaat tttggaaact
     9961 aatttcaata agccaagctg tccggtttat cagaatgtca atgcgaaaca ctcaatgaaa
    10021 attgatgaga taaaaatcaa tcttatcgct caactgacat cacctgtcag gtggactcag
    10081 acaattcaga atatggcaaa ggaaggagct aaagaattta ttgaatgcgg acctggaaaa
    10141 gtattgcaag gtttggtaag aaaaatttct cctgactcct taaccgccag tatctgataa
    10201 tctttgaatt ctgacctccg aagtatcttt gcactccatt aacaactgcg actgagtctt
    10261 acccaaaaga gggtgcatct tatcaggagc aatttcaact aatggcacta atgtaaattt
    10321 ccggcctgca atctgtggat gcggtatttt cagactttca tcctgaaaaa tctcatcata
    10381 ccagtaaaga atatcaatat ccacttcccg cgccccccat cgatctcttt gttttcttcc
    10441 aacaattgtc tcaatttcct taatttctgt aagtaaattc tggtgattta aaacttgaaa
    10501 ttcatgaatt accacctggt tcaggaacca ggcttgattt ttattgcccc atggctctgt
    10561 tttgtagatg gaagagttaa ttatgggcgg gccaaaaatc ctggttaatt ccattcgggc
    10621 cttttcaata aagtaatttt gatcctcaat gttgctccct aaaaggagaa ataatccatc
    10681 catcatcttt tccaccagat agtccaactt taaagtaata tagttagaac atacatctat
    10741 ttttgctgtt agtaatctta tttaagcgat gaattttctt aaaacattaa tggctgtgct
    10801 ggtaggactt atcctctata caggaatcat gttctttgta ctattaggca ttatttctat
    10861 tgctgcccag gaagaagaag ttaaagtgga ggagaactcc gtgttaaaac taaatctcaa
    10921 ccgaccaatc ctggaaagag ccatcgatga cccattcggg atcccatttt tctctattcc
    10981 gattgaaagc acaagtggac ttgttgaatt gaagcgcgcc atttccaacg ccaaagatga
    11041 tcccaggata aaggggattt atctcgaagc acccttcgta attacagggt ttggcaaact
    11101 ggaagaatta agaaaagcta ttgaggattt caaaacttcc ggaaagtttg taattgcata
    11161 ttccgatttt tacacggaag gcggatacta tatttcaaca atcgcggacg aaatatatgt
    11221 taaccctatt ggcattttcg agttcaatgg ccttagtgcc gagatcactt tttataaaga
    11281 aatgctggca aaacttgaag ttgacccggt aatattcagg gtgggtgatt ataaaagtgc
    11341 agttgaacca tttctgagaa gcaacatgag tgaagaaaac agagaacagt ataaatcgtt
    11401 tatcaattcc atctatgctg agataataaa taaaatggca gagtcaagaa ggatttcgcc
    11461 acaagtcttg aggcaaatgt cagatgatct ggaaatcaga ttggtactta atgccaaaga
    11521 aaggggattg gtagatgatc taatttatca ggaccaggta tttgatataa tacgtgataa
    11581 aataggaata accgatgaca aagaaataaa ttttatttca gcaaacagat atcataaatc
    11641 aataaaaagt aaatattctc aaaacaaaat tgcagtaata gtcgcttccg gagaaattgt
    11701 catgggcaga ggcgagcagg atatggttgg tgccgaagtt tttgtaaaag aaataagaca
    11761 agcgaaagac aacaaccgga tcaaggcaat tgtcctgcgg ataaattccc cgggaggaaa
    11821 ttacatagca agcgatattc tctggcggga aattaagcag gccgcgaaga ttaaacccgt
    11881 tatagcaagc ctttcggatt atgcggcatc cggcggttac tatctggcaa tggcctgcga
    11941 tactattgtc tcggattact caaccattac cgggtccatt ggtgtattta gaatccagtt
    12001 tactattcag gatttcctca agaacaagct gggtattaca agtgacaggg taaacacagg
    12061 taattattcc gacatgaatt cagtgctgcg tcccatgacg gatgcggaaa ggcaaatcat
    12121 tcagaatgaa gttgatgaag tgtacgaagt ctttgtttcc aaagctgcgg aaggaagagg
    12181 gttaagcgaa gattccatta gaaaaattgc cggcggcaga gtatggacag gagcgcaagc
    12241 cctggaaaat ggtttggttg atttgctcgg aaattttgaa gacgcggtaa aacttgcagc
    12301 ccaaatagca ggagtagaaa atgactattc aattgtttat tatcctgagc aaaagtcact
    12361 tttggaagaa atcctttacg aaatgggaaa tgaagccgaa gtcaaatttc ttaaacaaga
    12421 aatgggtatt ttgtatcctt atgtggaaca attaaagaaa ttggaatacc tccagggcat
    12481 acaggccagg atgcctgcag aattaactat ttactagcct cccaaatctc catatttttc
    12541 ataaatgtct tacttttagt ctatggctaa aattagaaac gattggagtc gtgacgaaat
    12601 aagcgggatt ttcaactcac ctattcttga tttagtttac agggcagcat ccgtccacag
    12661 aaagtatcat gaaccacgag aggttcaagt ctgtactttg ctttcggtga aaaccggtgg
    12721 ttgtcctgaa gattgtgctt attgtccaca ggcggccagg tatcataccg aagtcaaaat
    12781 ccataagttg cttccggtgg tggaagtaat agaatcagcc agaaaggcga aagagtcggg
    12841 gagtacaaga ttctgtatgg gcgcggcgtg gcgggaggtg cgggataaca aggattttga
    12901 tagtgtatta gaaatggtga aaggagtaaa ttctttagga atggaagttt gttgtacact
    12961 gggtatgtta acaattgaac aagcaagaaa gttaaaggat gcgggtttat acgcatataa
    13021 ccataatctg gacacaagtg aggaattcta tggcgaaata attactaccc gcaattatga
    13081 agacaggttg aatactcttt ctcatgtcag aaatgcaaag atttcagttt gctccggtgg
    13141 tatcataggc ttgggggaga atctgaatga tcgcattggc atgctgcata cactggcaac
    13201 attaccaaaa catcctgaat ctgtcccggt caatgccttg gttccggttg aaggtacgcc
    13261 actggaagat caggaaaaag tttcagtttg ggaaatgatt cgtatgattg caacagcaag
    13321 aattataatg cccagagcaa tagtgagatt atcggccggc cgggtaagaa tgacaatgga
    13381 agaacaagca ttatgctttc ttgccggcgc aaattcaatt ttttccggtg ataaacttct
    13441 gacaactccc aatcctgagg aatctgccga taaggaaatg tttcagatat taaatcttaa
    13501 gccgaggaag gctttcaaga atgctcccga aattcccagc ttaatgtaag tttctatgaa
    13561 gcagggaaat gtgcttcagg ataaaattag ccggagattg gctgaaaata acctgcgggt
    13621 cttaacaaca tcccttccag gaattgattt catttcaaat gattacctgg gtttagcgag
    13681 atctcaaaaa atggctggaa aaatttcaga atacatttct aaaatacctt acagaaagaa
    13741 tgggtccacg ggttcgcgtt tgctttcagg taattttgaa gagttggaaa aattagaaga
    13801 aagaattgct gacttttttt atgtcgaagc cgcacttttt ttacccacag gttatcaggc
    13861 caacctggcc ttgatttcag ctcttggagg caaggatgac actttaattt atgatgaact
    13921 ttcccatgct tccattaaag acggcgccag acttagcctg gcaagaaaat attcatttcg
    13981 acacaatgat tcagatgacc ttaaacggaa gctcagccaa agcagcggca ataagtatgt
    14041 aattgtagaa tccatttata gtatggatgg cgaccatgct aatcttattt caattctcaa
    14101 cattactgaa gaaaatgagg catttttaat cgtggatgaa gcgcattcta ctggcttaac
    14161 agaaaaaaat ggagcgggca tgtgtgttga gatggatata gccaatcgaa tctttgctcg
    14221 tatacatacc ttcggaaagg cagcgggagt ccatggtgga gccatctgcg gaagtaattt
    14281 actcagggat tacataatta atttttcaag accatttatt tattcaaccg gaccatcgct
    14341 tcaccaggtc atttccgtgc aggcttcgct ggatgaattg gaaaatgcgg atgatgccag
    14401 ggcagcattg aatgatcgaa ttacgttttt tcatgatgaa ataaaaaaaa tgaattcaaa
    14461 acttcaattc ctgaagagca atagcccaat tcaggcgatt ataattcctg ggaatgatga
    14521 agtaaaaaga atagcaggca aaattttgga acaaggattt caggttaaac ctattctggc
    14581 tcccactgtt aaaagaggta ctgaacgaat aagaatatgt ctccattcat ttaataccga
    14641 agaagaaatc agaaaattaa tttcagtagt atcaggatga attactttgt aaccgcaatc
    14701 ggaacggata gtggcaaaac tgttgtcagc gctgttttgt gtgaagctct taaagctgga
    14761 tactggaaac ccattcaggc gggtgaaccg cgggattctg aattaattaa agagttggtt
    14821 tctgacaaga caatcaaaat tcatccggag aagtatttat taacccagcc ggcatctccg
    14881 catgctgctg ccaggcatga cggaattaaa atttctcttt ctggcttttc gtcaccttgg
    14941 tcggagggta atcttattat cgaaggtgcc ggtggcatat tggttccgct caatgaccag
    15001 gatttggtta ttgatttggt aaaacaattt cgtgcgaaaa ttattcttgt atgtgacttg
    15061 tatcttggaa gcataaatca cagtctttta acaattgaag ctttgaaaag cagaaatctt
    15121 gacgtaaaag gaatagtatt caatggattc aatcggcaac cctttgaaat ggaaagcatg
    15181 gaaattattc tgcacaaatc cggttttaaa aaactgctgg taatgaataa ggagagtaaa
    15241 attacaccgg acctgattaa aagctgggcc aaacagatcc tggaaacctt atgacgtggt
    15301 ccgccaaaga tcagaaatat ttgtggcatc ctttttcgcc gttacaaccg aattccccgc
    15361 acttaatagt tacgcgtggg gaggggtgtt atttgatttt agaggatggc aggcgaattc
    15421 ttgacggaat ttcaagttgg tgggtgaatt tgcacggtca tggtaataag aaaattgctg
    15481 aggcaattaa agatcaggca ttgaaattgg aacaggtaat ttttgccggc tttactcatg
    15541 aacctgccat cagcctcgct gaaaaagtgc tttttctatt gcccggtaat caagctaaag
    15601 tattttattc tgatgatggg agtacttccg tagaagtagc attaaagata gccatccaat
    15661 actgggaaaa tcagagaatt aacaaatcca ggattatagc ccttgaaagt gcataccatg
    15721 gtgacacatt tggagcaatg gcggtgggag caaggggtat ttttacgaaa ccatttcaaa
    15781 gtcgcttatt cgatgttgat ttcatttcaa cacctggcct gaatgaagga gataaagtaa
    15841 tctcagaatt caaaagggcc atttccggaa atgataccgc ggcttttata ttcgagcccc
    15901 tggtgcaggg agctgggggt atgttaatgc atgagccaga gattttgaat gaaatgatcg
    15961 caatggctca gaaacaagac atattatgta ttgctgatga agttatgact ggatttggac
    16021 gaacagggaa aatgtttgcc agcgactacc tgacttctaa accggatttc ttttgcctgt
    16081 cgaaaggatt gaccggaggt acgcttcctt taggtattac tacctgttct gacaaagtag
    16141 cgagccaatt ccctgaaaat aactttgaga aaacatttta tcatgggcat tcttttaccg
    16201 caaatccaat ttcctgtgct gcagctttgg caagcctgga aatcttaacg agcaaggatt
    16261 gtctaacgtc tattgaacag atttcaagat tgcatgctga tttcattaag aaaaattctt
    16321 tcgatttcct atgtaactta aggcatagag gaaccatttt agctatggat gcaaaaacat
    16381 ctgatgtacc gtcctatcag aatccgatta gaaaaaaaat ttatgacttt tttattgaaa
    16441 ggaatatttt gctgcgtcct cttggcaata ccatttatat tttgcctccg tacattataa
    16501 ctgaaattga attggcagga atttatgctg caattagaga atttgagaat actttgaaac
    16561 agtaattgta gattacaaag cgaataattc ttaaattaaa cattaaatcc accatttatg
    16621 aaaagcataa ttcctttcct gctgaccatt tttatctcca acggtatttt ttctcaggtg
    16681 gaagacctgg gacaaattga attccccaat tcgggaagtg cagaagctca ggaaagtttt
    16741 attcgtggcg tattattact acatagtttt gaatatgaag atgcagctga ggagtttagg
    16801 agcgcccaaa aaactgatcg tgattttgca ttggcgtatt ggggtgaagc tatgacctat
    16861 aaccacccga tctggctcca gcaagataag gcgaaggcac taagttcact tgaaagattt
    16921 gaaaaaacct cagaagagcg gttggcaaaa accaaatcag aaaaggagaa aatgtggctt
    16981 gagtccatag aaatattata cggaaatgga actaaagaag aaaacgattt tgaatatgaa
    17041 gattttatga aatcattttc cgggaaattt cctgacgatt tggaaatcgc ttccttttac
    17101 tccctctcaa ttcttggaac ttgtcatggt ggacgggata taccttccta tcacagggct
    17161 gccgcagtag cagaaaaaat ctacgagata aacccaaacc atccgggagc tcttcattac
    17221 attatacatt cttatgatga tccggaaaat gcggtcaaag ctctggatgc cgctaacaat
    17281 tattccaaag tggcgccgtc cgcgtcccat gcattgcata tgccaaccca catctttgtg
    17341 gccttgggaa tgtgggagga tgtggtaagg gaaaatatcc ggtctgcgaa tgcagcggat
    17401 gcaagaaggg aaagaaaaaa tctcggcctt gatgcccggg gatatcatgc cctctggtgg
    17461 cttgaatatg ggtatttgca attgggtaaa gtaaataagg ctcgtgaaat gcttacccaa
    17521 atgcataagg aagctttgga aagtggttca caccgaacca gaatgcattt ttcggtaatg
    17581 aaagcggctt tcctggtaga aactgacaat tggagcgagg atgtattaag tatgaaagtt
    17641 gatttggccg gtatgaataa ctgggtgaaa gtactaaatg gatttttgga aggttatggc
    17701 acaatcagga ataacaagat tgaaagggca aagagtattt tggacgacct taagaaaata
    17761 aatacaggca ttagtagttt ttctgaatat gacttaaatg aaaaggaagc tgcaataatg
    17821 gtttcgattc ttgagagtta ttatttattc gaatctggta agacgacgga aggcataaat
    17881 ttgctaaaaa aggcccgtga ggcggaagaa agccttagct attcattcgg cccgccggct
    17941 ataccgaaac catctcatga aattctgggt gaaatttact tgcatgaaag aaattttgaa
    18001 ttagctgaat caaattttag aatttcactg gaaagggcgc cacaaagagt aatgtctctc
    18061 aaagggttat atgctgcatt aaaaggactg ggcaagtccg gccaggcaga agaagtgttg
    18121 gctgaaatta agaagatcag gaaaagtgcg gatcaggatg tctatcccaa ttagaatcaa
    18181 gcatcctgat ccaaattatc tattctttat atttttcaaa ccacccaaga attgcctgaa
    18241 cttttgcaat caggttactg ggtctggaag caattccatg actggcgcct ggaattctaa
    18301 ccattgcagt ttcaaccttt tgaattttca aggccgcata aaattgctct gattcagcaa
    18361 tcggtgttct gaaatcagat tcacccgtaa gcaacatggt aggggtagta acattgccca
    18421 cgtaggaaat aggagatctg cgcatgtatt gctcaaggtt ttcccatggc gtgcccggaa
    18481 accaatactt ataaaagaaa ggtattccat ctgagtaaag cacgaaacta taccagttta
    18541 taacgggctt tgctacaaca gcagccctga agcggttcgt atggcctaca atccatgcag
    18601 ttagaactcc gccgccgctt ccaccggtaa catatagttg agtctcatca acgtaacctt
    18661 tggcaacagc agcgtcaact cctgacatca gatcttcata atcgtgattg gggtaatcat
    18721 gatggattag atttccgaat tcttccccat agctgctact gcctcttgga ttagtgtaaa
    18781 gaactacgta tcctgccgcc gcgaaaagct gaatttcagc cgcaaagaat ggactgtaag
    18841 cggcaaatgg tccgccatga atttctaaaa taagcgggta tttctttgaa ggatcaaaat
    18901 ctggcggtgt tacaatccag ccatgaatcc gcttcttatc aaatgacgat tcccaccaca
    18961 gttcttcaac tttgcctaac tttttgtaag cgaacagatc atcgttcaga cgagttaatc
    19021 tattgacgtt attccgttct cccgctccta aatcagacgg atggtccgga ccacctaaag
    19081 tatatgcata tctgccagtt gcagttacag aaaaagttcc cgcggtataa ggcctgccaa
    19141 gggaaagccc gcctacataa ttggtgagct cagaaacctt tccgttcaaa tccatatatg
    19201 cgatctttgt attccctaca tcatcgtaag caatataaag ccccttatta tctccgctcc
    19261 agtaatactc ctgaatatcc cgatcgaatc ctttactgat aagtctggag ttggttcctt
    19321 caggagacat aacataaagt tgcgttagtt ggtacccctg gtaccgatca tcaaatcctg
    19381 agtaagcaat taatttgcca tcgggagaaa tttcaggacc gccatcgggt ccctttctgg
    19441 tggtaagcgg cgtaatttta ccgcctgcaa cgcttacaga atatatttct gaatttcttg
    19501 cttcttctaa ttcccggttc tcatgcatat tcgatgtgca aattaaattt ttgccatctg
    19561 cagtccattc aggattattg aaattgtacg gaccagaagt tatttgcctg ggtgttcccc
    19621 catctgccgg cagagtaaat aattgattat atccctgttt aagatatccg cggccgtcac
    19681 cccggtaaga aatatcatca atgtattttg gagcgtcatt ccattttgca ccatcgggtt
    19741 tagcaggcat tttcacaaat ggtttttccg gttcttcgac aaacatggag aaagcaataa
    19801 atttattatc ccgtgaccag ctaatgccgc ccggtgaatt ttgaagattg gttaacttac
    19861 tttctgctcc tgtatccagc cagcgcaaat aaagttgaga actgccctcc ttgttcgaca
    19921 tatataaaat ctgacggcca tcaccagacc atattgggtt gtaatcattt tgaatacccg
    19981 tagtgattgg ccggttattg gtgccgtcaa aattaatgac ccagagattt gataaatttc
    20041 tgtccgacat aatgtcttta aaatttcgag tgtagataat tctgtttcca tcgggagata
    20101 tttgagggtc cgatacatac tccaaatcga atacatcaat gggttcaagc ttgtctgaaa
    20161 tttgcccttt agcagaaaaa gaaataatga gtaaaagaac cgatagattt ttcatattgt
    20221 ggttaagtta agtaggcaat ttatgaacga aagtgtgaat tgccgggaaa agggcaatca
    20281 ctccgtgtca atacagtatt ttatttctta tatagatcaa ctttttttgt tgtcagccct
    20341 ttggaaacga ggtagtcata aagttccttt gctttttcac cccccttttc tgcatgatgt
    20401 aacatggtga gaccatgagg gcctttcgca ttaataagac ttgggtattc ttccaaaatg
    20461 ggtcttacaa gttccgtttt tccaagcatt gtcagcacga aaatgtttgt tcgtgccccc
    20521 tggcctatca ggtattctgc aatttcaata ttacccatat gaccagctcc gccaattgca
    20581 gtctcaaaat ctccaccgcc ccaatcccag gtgcaattca ataaattagg atattctccc
    20641 aacatctctt taacctttgt taagtctgtg tgaccagcgg ctacaaaatc tagtgtaagt
    20701 tcttgcttgt aaggctcagg ccgctgattc tgcctggcca aagaaattac cggtgcaaag
    20761 ctaattccca aagcccctgc gccggcaagg gataaaaatt tgtttctttc catcgtaaac
    20821 ttttttaaat aagttaacga aaattatttg ctgaaaatgg aatttttatt taggcggaga
    20881 ttaaattttt tcaatttacc aaatgcttca ttccgctccc ggtatttagt atcagaattt
    20941 gtgcggatgg cgtgatgaat ccgctttcaa ccaatttgac taacccggca aaagcggaag
    21001 caccttcggg agatacgagt attccttctt tttttgtgag tcccgttagt gcaagccgta
    21061 tctcattttc agaaacagct actgcggcgc ctccagattc tttcagacat tgcataatca
    21121 gttctaagcc aaatggatta ggaacgttaa gccccaacgc aatggaagcc tggtagcgag
    21181 agggatttat agctttattt tcccaggcat ccacaaccgg actgcagatt tcgctttgca
    21241 ctgcaatcat ctttggtaaa aggccttcaa tccaacctaa ttgctgcaat tcacggaatg
    21301 ctttccagat accgatcagt cctgtaccac cgccggtggg ataaacaatt gcatccggca
    21361 atttccaatt catggcctct gcaatttcat agcccatggt tttctttcct tcgatccggt
    21421 agggttcttt aagagttgat acatcgaaat attcgcccat tgaattaatc tccatcactt
    21481 tcttaccgca ttcgctgata aggccatcaa ctaatattac ttctgcacca taggctttac
    21541 actcatcaat ataaattttt ggggtgatgg caggaaatac aaccgttgat tggatgttcg
    21601 ctcttgcgca ataagcagac atagcaactc cagcatttcc agcagacggc gtgatgcatt
    21661 tttttatccc aaattccttt gctttagaga tcgcaacact catccccctt gctttgaatg
    21721 aaccggaagg attcaaggct tcatccttaa caagaatttg tttttctaag ccgtcaattt
    21781 tcgaattcag tgaaaatatt ggtgtaccgc cttcacccat ggtgatgata ttttcaggat
    21841 ttaacacggg taatagctcc tggtatcgcc acatggtcca gggtttgtct ttcaaaacct
    21901 ctcttgaaag aaccaattcg tacttattta taagagttgc tttgcaacat ggagatagtc
    21961 cgtaaagctc agagggtgaa taggttgcag aacatcggga gcaacatagt tctgtcaata
    22021 gtgattttga cctcgtttcg acattgaaaa tcatccccca aagtttgttt gatgtaataa
    22081 attttgcaaa tgaccttttg ctataggcta taactatttg ttatagtaac ttctggcaaa
    22141 tctcacgaac tccttataca caggcatgct tctttgtccc ttgcgttgaa taaactgaaa
    22201 tttccttgaa attgataaac cctgaatttt tatctcgacc aattcaccgg atttaagttc
    22261 tttcataacg gagcgacggg gaagaaaacc aagacattgg tctgccagaa taaaattctt
    22321 aagtccttcc gtgcctccta accttactat cacttccaga ttagataatg agatcccttt
    22381 tttttccaat tcggatttca ctacttccaa agtgcctgaa cccctttccc tcaatgcaat
    22441 tggtgtactt ttcaattctg acaacgatat attcgatttt ttagtcagaa aactcttttt
    22501 ggagcagact ggaattactt catccgtcat gaattgggaa taatcaaaag agctctcttt
    22561 atgatggctt tcaataattc ccaggtcaat ttcattattc gcaagtgctt caataatctt
    22621 ttcagtattt ctattgagta aagttactct gaatccggga ttcttttgaa gatataaaga
    22681 taacaccggc ggaattacgt acaatgaaat agtggtactg gcccccagca gtaattcaat
    22741 ctggggctcg aaatttttac tcacctttga aagttcaagc tcaatctctt tttgcttatc
    22801 taacattaca gcaagattct tatataatat agcgcctgcc ggagtaagtc caattgagtt
    22861 tttgtttcta aagaacaggg cagttttata ataatcttcc aatgccttaa tatgattact
    22921 gactgcgggc tgagtaataa acaaattctg actggcacgt gaaaagctaa gctgccgggc
    22981 tacttccaca aaaacctgat gccggaaaga aatcatttaa tacttattgg ttaaccaaaa
    23041 ttaggagttt aattttagag aatttcaatc ttaattcaat accttgaaaa atacccgatt
    23101 cgagcactta cccacgaaaa tttttaattc aaataaggat gcttcccgat atgcggccct
    23161 ggaaatttct gatttgattc gaaagaaagc agcaaatagt gaaatctgtg tccttggatt
    23221 agccacaggt tcaactccaa tttctatcta taatgaactg gttcggcttc ataaagagga
    23281 aaaattaagc ttcgccaacg ttattacttt caatcttgat gaatactatc caatgcagcc
    23341 tcatgcgctt caaagttatg tcaggtttat gcatgaatat ctatttaatc aaatagacat
    23401 aaataagaac aacattcata taccggatgg tacgctgagt aagtccagag tgcaatctta
    23461 ttgtaaggca tacgaaaaga aaattgcaga gtacggtgga atcgatttgc aaatcctggg
    23521 tatcggaaga acgggtcaca ttggatttaa tgaaccaggt tctgatatca actcagagac
    23581 caggctgatt acattggatt cgattacaaa aaccgatgct gcaagtgatt tttttagtga
    23641 agaaaatgtg ccttaccagg caattactat gggagttggc acaattatga agtctggaag
    23701 gattttacta attgcttttg gagaaggtaa agcaggcata attaagaaag ctgttgaagg
    23761 aaaagtggac gagcaagtac cggcgtctta tctgcaaaag catcacaaag ccgaggtggt
    23821 gcttgatgtg gctgcagctg atgaattggt tagaattaaa tctccgtggc tggtacaaac
    23881 ctgcaagtgg gatgataaac ttattaagaa agctgtttgc tggctctctt taaaacttga
    23941 caaacccatt ctgaagttaa cggacagaga ttatattgac aatggaatgg acaacctctt
    24001 aattgaacga ggctcagcat ataagatcaa tattgatatc ttcaacaaat tgcagcacac
    24061 tattacaggc tggccgggag gtaagcctaa tgtggatgat acttaccgac ccgagcgagc
    24121 tgcgccatca aagaaaagga ttttgatatt tagtcctcac cctgatgatg acgttatttc
    24181 catgggtgga acttttatca gactgttcga acatggtcat gatattcatg ttgcctacca
    24241 aacatccggc aacattgcgg tttttgatga tgatgtcatc aggtttgcgg atttttcaaa
    24301 tgaattcaac aaacttttta aagtaaagga tccacagtta acgaaacttt ttcagcgagt
    24361 aagcaatttc attaataaga agaaaccagg cgaaattgac aatgaggagg taagaaaaat
    24421 taaaacattg atcaggagga gtgaggcgag atctgcctgc aggtatattg gtcttgatat
    24481 aaataacgct cattttctcg accttccatt ctacgaaacc ggaacggtta agaaaaagcc
    24541 attgggagaa aaagacatta ccataactgt taatttgctt cgtcagctaa agccacacca
    24601 aatctacgcc gccggtgact tgtctgatcc ccatggaact cacagggttt gccttgaagc
    24661 aattattggc gctcttgaag cgtgtaagaa agattcatgg ctgaaaaatt gttatgtatg
    24721 gctgtacaga ggtgcgtggc aggagtgggg tatccatgat attgaaatgg cagttcccct
    24781 aagtcccggt gagttgctga agaaaagaaa atcaattttt aagcatcagt ctcaaaaaga
    24841 ttccgcgctt tttcctggtc ctgatgaacg cgaattttgg gagcgtgccg aggcccgaaa
    24901 ccgggcaact gcagatattt acaataaatt aggtctgcct gaatatgaag ccatagaagg
    24961 attcaaaaga tattattttt agtaaagaaa ttacttcatt caatagacat aattttctat
    25021 cttcactgaa ttatagacct cttaaaccca tgaaattttc cgaattaatt tccatgtttc
    25081 gagcaggcaa agccggagcc aaaagccaca tgaaaaattt aattgaaatg gcagccgcgg
    25141 atggcaattt tgatgatgta gaatataatc tgctcaagca aatagcaaaa aaacataaag
    25201 tttccgaaaa agaattgaaa aaaattaagg ataatccaca gaatgttgct tttgaagttc
    25261 catccgaccc caaagaaaag tttgatcagc tttatgatct tgttaacatg atggttatag
    25321 acaattatgt agacagagag gaggtaaacc tatgcctgat ttttgccggc aagtttggct
    25381 ataaaaaaga aagaattgtt gaactggttg attctatcaa ggataatatt aagaataacc
    25441 aggatcagga agcaacaagg aaaaggctcg actggttgct taattagtgg ccttttcaga
    25501 ttctttggct aattcactag gccttccaaa gatgtacttt ctggcttcac tgagatttct
    25561 ggcctttgaa acatccttat aaatgtccct ccattcatgg aaaaccagag taatcggatt
    25621 ataggttcct accggttttg ttataccata tatcgcctgt tcttcctcag gttgaaaagt
    25681 tccaaacatt ctgtcccaaa taattagcgt agacccataa ttcttgtcca tgtacttttc
    25741 attggtcgca tggtgaactc tgtgatgtga aggtgtggtg aaaatgtatt ctatcggacg
    25801 aggtagtttt cgaatatatt cagtatgaat ccaaaattga taaaggactt ctatttggtg
    25861 gcaaataaaa aatattaccg gatgaaaacc catcagggca acaggtacga agaaaataaa
    25921 ttttatatgc tgaacccagc ttaatcgaaa ggaaaccgac caattatact tttccgaatt
    25981 gtgatgtgtt acatgagttg cccaccagaa cctgttttca tgtgcaatcc ggtgcgccca
    26041 atatctgcaa aaatcaatgg caaccagaca taggatataa gaccaccaaa ccggaggaat
    26101 gctccatgga accagattca ggaagaaaag aacggatgca aataaggctg tcttaattat
    26161 ggcactcacg ataaggttcc cgattccaat agttgctgca gcaaacagat ctttcttatc
    26221 gtataaatcc cttttttgat aaacagaaat accccactca gctaaaacaa gcatgatcat
    26281 aacaggaaca gcatacctga taacttcagg ggcccccata tctaatactt gctgcaagct
    26341 tagtttctct ttttccataa ttgacaaaag taaggcttag ccttcagaac tattagccaa
    26401 aaccatcaaa ctttaaaaaa catttcttat ttcaacattt agaatacttc atttacccca
    26461 aagtagatct cgttactttt actttcgcca aagccaaagt ctggccggag tattggtatt
    26521 tctgcaaacc aggtttcctt cagaataata cttttcattt ctgaaatata cctcgggtat
    26581 aaaatcaaga atgaattgtt tttcggtaat aaccctggcc agcgaattgc attgtgaaat
    26641 ccgtgaaagt agaatcactc ccccgggttc taaatagaaa ccgaccttcg ccaccaattc
    26701 ctactgtgtt tccggcgcgt aacttagcaa gggattaata gtataactgg catcatttta
    26761 tttggacaag gaacaaagtt gaattcagga ttaggcgcaa aataagaaaa ttgtccaatt
    26821 taggacacca gatgaaataa acaaccttga ttttctaatt caaaaatgaa ttaacaagat
    26881 tacctttgtc tgacctatgc gtatgaacat ttgtttaaac taaagtgatc gtcttatgaa
    26941 aaaattattt accattttat taacagggct gatgattgtc ggtatgacaa tacaaagtca
    27001 ttcccaggga tttcaatttg ggattaaagg tcttgcccaa gtatcatcta tacatgggtt
    27061 agatgataca ggggtatccg atgctaagag caagattact acgggtattg gtttgtttgc
    27121 gaccataaaa ctgagcaaaa ttggcgttca gccggaattg caattattga agcaaggcag
    27181 caaattcgaa gattcgggag gcactgaata caaccagaaa atgacctact tcaaagtgcc
    27241 ggttatgatt aacttatata tagtaaagca gtttcatctt gaagcaggtc catattttgg
    27301 agttctgttg gctgctgaac aaaaactaac ctccgaccct ttgaatttgg gaaccggccc
    27361 gattgacaat aaagacaatt ttaaaagtac ggatgtaggc attgcagccg gagttggtgc
    27421 agatatcaaa aggttgaatc tttccttcag gtatatgctg ggactaacag atatcaatga
    27481 ttcctcaggg accactgaga agcttaaaaa tggaatcttc cagtttggaa ttggcatcag
    27541 cattattaat aaagactaga ttttattact gggataatat ttcagagggg cttttcagcc
    27601 ccttttttat tgccataccc ataaataaag tattatttcc caaaaaattt cttgcctaaa
    27661 attaaaataa ttcagtatct ttaaccgttt ggttaaatta tatagttaaa ctaaatggtt
    27721 aaagacgaaa ctaccgaaca atctattcta aaatctgcca gggagacatt taagaccagg
    27781 ggattttcag gcgctaaaat ggcagaaatt gcaaaggaag caggaataaa caaggctttg
    27841 ttgcactatt actttagatc caagcaaaac ttgtttgata cagtcttcgc tgaagccgtc
    27901 tcagttttaa ttcccaagtt gattgccttg cttcaaagcg acctcccact tgatgttaaa
    27961 atctacaaga ttgttgacta ctactacctg acgctttcaa aaaatcgcga tcttccaatt
    28021 tttgtattga gtgaaatcag aaccaacccg ggctttatgc tacacatgct caaggaaaac
    28081 gggatggatc atttagttct ggataaacag atcgagcagg aaatcagcaa gggaaacatg
    28141 ataccagtct caactccgga attcttttcc aatgtaatgg ggctgatatt atttccattc
    28201 atagtagaac cgctgataaa agggttattt caaatgtctg aaatagattt ccaaaatttc
    28261 ctggaaaaaa gaagacacga gcttccatct caaattctaa aactgtttaa gttaaaatca
    28321 tgacaagcat aaaatcaaag ttgattttgc tggccgtggc tgctgtgcta tggctggatc
    28381 taaatggaca ggatctttct ttgcaggact gctatcacgc tgcccggaac tttagtccgg
    28441 taaaaaagca gcagcccaat attgctcaaa taagcagcct ggtaatggaa tcgctgaaga
    28501 aagaaaagtt acctcagata ttcgcgaacg gtcaatatac ataccaatcg gatgtagttt
    28561 catttcctga tttcccggga attaatcggc cggatattcc caagaatcaa taccggattt
    28621 cattagacat aaatcagagt ctctttgccg gtaatcggat caataaaatg gttgaaacgg
    28681 aaaaggcaag gctgcaagtg gatcaacaag aactggaagt aaagctttcc ggcatcaaaa
    28741 gctcagttaa tgaattattc tttgctgctc tttccctaca ggaaaaagag aagttatacg
    28801 atatcataat cggtgatctt aagacccgaa tcagtttaat ccaatcgctg attagaaatg
    28861 gcgttcttct tcccggaaat gaaaaatctt tgctgattga aattaagagg gcagaacagg
    28921 aaaaaaccgg attgtatttt cagaagtccg gtgtattgag aatgctggaa gagaaaaccg
    28981 gattggagat ttcttctgaa agaaatttgg ttgttccgga atttcaattc gtgcaaagta
    29041 acaatttgaa gcgtgaagaa aagttgcttt tcaaatattc tcacgcaaca ttgcaagaac
    29101 aaatgcaatt aacaaatctg agtcgctatc ctaaactttc agcattcgga agcctgggaa
    29161 ttggaaatcc caatccggtg aatttttttg aaaccggcac ttctgaattt tatctggttg
    29221 gactaaagct tagctggaat atctgggact ggaacaattc gaaacgggca aagcaaattc
    29281 ttcaactgca gcagcaaaac ctgaacgaag aagaaaggga ttttgatttg aatgtattca
    29341 ctcaattagc cggagccaat accaatcttg aaaagctccg gactttgctt atttccgaca
    29401 aggaaattct tgaactgcaa aaggaaatta ccgaagaagc attctcgaga ttccgggagg
    29461 gtgtaattac tccttccgaa tactcaacgg aattaaacag gcaaattcag gctcagttaa
    29521 aactaaaaat gcgagaaatc gaaatctctc aggctatagt gaacatactc acattaacag
    29581 gacatattta aatacacttt taaattatga aaaggaatat caaaatagta ttgtttctcc
    29641 ttccactggc ttcctgcaat tataacgctg aattggctga tgcttatggc aattttgaag
    29701 cagacgaaac agttatttcc gcggaaactg ccggtaaact attgtacctg aatgttcagg
    29761 aaggtactga aataccgtca ggtttagatg tagccctggt ggatacaacg caacttttcc
    29821 ttaagaaaga acaattaaaa gcgggtataa acgcgataat aagcaaggtt cagcctgttc
    29881 aaagtcagat caacgtttta atagaaaaac gaagaaacat tgcgcgtgaa aagaacagga
    29941 tagaaaagtt attggccgat agtgcagcaa cccggcaaca atgggacgac atttcgggtc
    30001 aactggagtt ggttgacagg gaaatgactg ctacccggga aaggcttgaa tcggcgaata
    30061 aaggaatcct ttccgaaata aaatctttgg aagtgcaaat tgcccagatc aatgatcaaa
    30121 tagttaaatc acttgtccta aatcccgcac agggaactgt tttgcaaaaa tttgccgagc
    30181 ccggcgaagt cactgcattt ggaatgcccc tgtataaagt tgccttgctg agtcaacttt
    30241 acctgcgagc ctacatcagc ggtaatcagt tggctacatt gtcaatgggc caggttgtct
    30301 ctgttggtgt tgatgatgga gaaggcggaa taaagaatta ccctggaaga ataacctgga
    30361 tatcatcaaa ggcggaattt actcctaaag ttatacaaac cagggaagag cgagttaacc
    30421 tcgtgtatgc tatcaagatt ttggttgaaa atgatgggtt attaaaaatt ggtatgcccg
    30481 gtgaagttta ttttaattaa taattatggt tataagggtt caggaaataa gcaaacattt
    30541 taagacggta caagcgttaa aaaatatttc gttccaggta gcggaagggg aagtctttgg
    30601 acttattgga ccggacggtg ccggcaagac tacccttatc cgaattatgg caacactgct
    30661 taagcaagat gccggaaata ttacgctggc cgggtacaat aatatagaag attatgagca
    30721 ggtgagaaac ctgattggat acgtccccgg taaattttcc ctttatcagg atttaacagt
    30781 agaagaaaac ctgaacctgt ttgccactat tttcggaact actcttcagg aaaactatta
    30841 cctgataaag gacatttacc agatgcttga accgtttaaa aaacgtcgtg ccggaaactt
    30901 aagcgggggg atgaaacaaa aacttgcttt aagctgtgcg ttgatccata aaccaaagat
    30961 tcttctcctg gatgagccaa ctacaggtgt ggatccggtt tcaagaaaag aattttggga
    31021 aatgcttggg aggttgaaaa atgaaggaat aactattctg gtcacgacgc cttatatgga
    31081 tgaggccgct aaatgcgaca gagtgggttt aatccaaact ggaaatattt tgtccataga
    31141 tacaccccaa aatataataa caggatttcc cctgaagatt tattctgtca ggtcattaaa
    31201 acgggtagaa ctacttcatc atttaagaga tatgccaaat gcgcattctg caaatctttt
    31261 tggagattcc attcattata tccagaaaat gccggagcca gatgaattaa ctctggaggc
    31321 tgctttgaaa caggcgggtt atctggacgc gaaagtggaa cggattcaac ccgtaataga
    31381 ggattgtttt atagaattaa tgaaatctca aatcgcagcc taatggttat agaagttgaa
    31441 aacctggtga aaaagtttgg tgatttcact gctgtggaca acatcagttt tgaagttaag
    31501 gaaggagaaa tttttggttt ccttggtgca aatggagctg gtaaaactac ggcaataaag
    31561 atgctatgcg gtttaatggc tccaacatca ggtaggggtg tggtggccgg attcgatatt
    31621 aaaacacaaa gagaaaaagt caaaaggaga ataggataca tgagccaaaa gttttcgttg
    31681 tacgatgatc tctcatccta tgaaaacatc atgttctatg ggggtattta tggtatccgc
    31741 aggaaagagt taaagaaaaa ggcggagcgt ttaatcagaa aattagggct cgaatcagta
    31801 gagaataaac tcctgaaaag tcttccaccc ggatggaaac agaaacttgc attttcagtg
    31861 gctttacttc atgagccgag cgtaattttt ctggatgaac ccaccggcgg cgtagatccc
    31921 gtcacaagaa gacaattttg ggaattgatt tatgaaactg ccacagaagg aaaaaccata
    31981 ttcgtgacca cccattatat ggatgaggcg gagtattgtg atcgggtatc tataatggta
    32041 aatggaagga ttgatgccat ggcatctccg gcagaattaa aacagaattt cgaagttacc
    32101 agtatggagg atgtttttct gaaacttgca aggtcatgaa acggtttgcc ggttttttca
    32161 aaaaagaatt aattcacatc attcgtgatc cccgtaccct tattatcttg ttcggaatgc
    32221 cttttgttca aatattatta tttgggtatg caataactaa tgaagtgaag aatgcccgaa
    32281 tcgccatact tgataactct aaggatgaag tcagttttaa actcattgat aaaattctta
    32341 gcagcgggta tttcactatg tcggagtatt tgcagacttc agctgaaatt gaccatgcat
    32401 ttaagcaagg tgaagtaaag cttgcagtgg tcattcctga aagactttcg gaaaaattga
    32461 aaagaaatga gttagttcag attcagttaa tatctgatgc caccgatccc aatacgggca
    32521 acacacttat caattatact tccgcaataa tacgggattt tgaattttca agaagtgaaa
    32581 acttacgacc tgttttgatc aacccggaaa taaaaatgcg ttataatcca ggtttaaaaa
    32641 gcgtttatct cttcattccc ggaattatct cggtaattct aatgctggtt tccacgatga
    32701 tgacttccat ttctttaaca agggagaaag aactgggaac aatggaagtg ctgcttgctt
    32761 cgccaatgaa accggcccag gtgataattt ctaaagtaat cccatacctt tttttggctt
    32821 ttggaaatgc attgctcatc ctggccttgg ggtatatcgt atttgatgtt ccggttaagg
    32881 gaagtttaac acttctctta tttgaaagtc ttttgtttat agccgtagca ttgtctttag
    32941 ggattctcat ttctacaaaa accgactcac agcaagttgc tctgatgatt tctctttttg
    33001 cgctgatgct gccaaccata atattatcag gttttatttt tccagttgaa aatatgccgg
    33061 ttatccttca gataatttcg aacatcatac cggcaaaatg gtttatcgtg attgtgaagg
    33121 gaataatgtt gaaggggatt ggtattgctg aactttggaa agaaaccctg atacttgtgg
    33181 gatttatttt cgttctgata attgcaagtg taaaaaacta taaagtgaga ttggaatgag
    33241 agttttgctt atcatcattc aaaaggaatt caaacagata ttccggaacc ggggtatgct
    33301 tccaatcata tttgtattgc cgatcattca attattaata ttggctaacg cggcaacttt
    33361 tgatattaag aacataaaga ttagaatagt tgataaagat ctaagttccg aatcccgaag
    33421 gctggttagc aagtttgaag gttctccata ttttttagtt gaaaaatgga gtttgagcag
    33481 tttcaataat gaatatgatc tggatgttgg tacggtagat ttaattctgg agattcccaa
    33541 tcattttgga agagatttat atgaaacagg atttagcagt gttcaattat tggtgaatgc
    33601 catcgatgga atcaaagccg gcctgggaac caactatgca caatctgtaa tggtggattt
    33661 ccttggtgag tttggtgcca cagcaccagg aagtaagatt gctaaaccca ttgaaattat
    33721 cccggctaat tggtacaatg aagaactgaa ttataaggag ttcatggttc cgggtatatt
    33781 ggttcttctt gttaccatga ttggcgcatt tcttacttcc atgaacatag tgcgagaaaa
    33841 ggaaattggc actattgaac aattgaatgt tactccaata cataaaacgc aatttatttt
    33901 gggtaaactg gctcctttct gggttcttgg attgtttgta ttcggatttg gattattggt
    33961 aggaaaaata ctctatgata tccccatcga agggagttta tgggtactgt ttgcattcgc
    34021 aggtgtctat ctttttactg ttttgggtat tggattattt gtatcaacag ttacaaatac
    34081 tcagcaacaa gccatgttca tctcatggtt ttttctggta atctttattt taatgagtgg
    34141 attgtttaca gcaatagaaa acatgcctat gtgggcacag aaaataacgt actttaatcc
    34201 tgtaaggtat ttcatggagg tagtcaggct ggtattgctg aagggtgcgg gatttaaaga
    34261 agttcaaagg cattttgtaa taatggctgt tttttcaact gctgtcattt cgctggctgt
    34321 gttgagatat cgcaaaagct ccggctaagt ctgttttcct tcggaattat ttcataaatt
    34381 acttttgaaa tcaaaattct gtaccatgag cattaagaat acccgcaggg aatggttgaa
    34441 gtctgccgcg ctgctggctg caggtctgtc aaccgcgaat tttactcttt caaaccccac
    34501 aaagacacct gtttataatc catatacggg ggaatggatg ttgaatttgc cagaccaggg
    34561 gttaagagcc cggcttaatt acaacgagaa tccatacggt ccttctgaaa aagctttaca
    34621 ggcattaagg gatgcacttg gtgaatcaag cagatatgct ttttcaatgg ctgaccaatt
    34681 caaagaaatg attgccgaaa aagaaggagt ctccgcggat catatttttc tttctgcagg
    34741 ttccctggaa tttctggcat taacggcatt ggcgcatgga ctggaagggg gaaatatcat
    34801 ctctgcttat ccaacctttc aatcactgat ggataccgcg gctgcatttg actgtgaatg
    34861 gaagcgaatt cctgttgata gtaattataa acatgattta ggaagaatgg aggaggcagt
    34921 caacagcaac actaagctga tgtacatctg caatcctaac aatcctacag gaacgtttct
    34981 ggatacagcc gaacttgcag aattttgcaa gcgggtttcc aaccgtgtaa ccgtatttgt
    35041 tgatgaagcc tacacagaat ttcatcccga tccggaatca atcactgtga aacacttagt
    35101 taaggaaggt cacaatgtta ttattgcaaa aactttctcc aagatccatg gatttgcggg
    35161 attaagagtg ggctatgcaa tcgcgcagcc ggctaagata agaaaaattg aagcttacag
    35221 gttgcaaatg actaccatga ccggaccttc cattgctgca gccatggcta gctatcagga
    35281 tcaggaattt atgaatttct gccggaagaa aaataccatg gcacgggaac aaacttatac
    35341 aattcttaaa gatcagggtt atgagtacgt tccttctcac acaagtttta tgatcttccg
    35401 aattaatacg gagcccaaat cattcctgaa aggaatgcgg gatcagggag taggagtacg
    35461 cagttggact tttcacaata aagactggtg ccgtgtaagt atgggaactg tggaagaaat
    35521 ggaaattttt gggaatgcgc tgaaaatgat tagttaagat tgaaattgaa tatttacatt
    35581 ttcccgaagg gaacccggga attccatgtt tgagcctgcc tggaaacaga caggaccgaa
    35641 tgggttgagt tgggagagac aaattcaatc ttcaagaacc aggtaatttt gttggataat
    35701 gacaccctca tttatctatt cgctccaaat aaacattctt ctcctttaac ttggtaccgc
    35761 gtcttttaaa ggctactaat ttgtcatcct tcatcagcca aatttcatct tcatcgtact
    35821 ggtcgccctc ttcagtaatg attagttccc cttttgaatt aatgaaccaa cggttatcat
    35881 tttcatcttc tctttctccc attgcataaa tctctaacct gccatttttc agaaattcaa
    35941 accggatatc tatttcctcc ataatattcg caacgagtgc ttttaccccg cttgaaatta
    36001 catggcccag gaaagattca tcgacttccc gatctatata gtcttcgtca tcctcattat
    36061 cttcattatc ctcttccatt acaattacaa gtttccaggt gcctgttatg tctttatcag
    36121 tcagtttttt ctgtgaatgc ccaagtattg gaagtataaa cagggcgata agtgttaata
    36181 ttcttgtttt catggtctga aatttaatta atagactggt ttttccgcgt tttgttacaa
    36241 caagacattc aatatagatt taacgtatag gctgtgaaat tggttagaaa ccgaagactt
    36301 attttaatgc ggaaagttca agagtaaatt tggttccctt cataggcact gaggttacct
    36361 tgatagtgcc accaagcctc agtatagctt cctgaacaat atacaatccc aaaccagacc
    36421 cgcctgtatt ttcagaggct ctgtaaaaca tttcaaaaat ccttccgatg tgagcttctt
    36481 caatcccaat tccgttatcc tcaatttcca gaattagatt ttcccttgcg atatgggctg
    36541 atattctaat gaatggttcg tttctacccc ggtattttat agaatttgaa atcaaattat
    36601 tcaaaatgat tttgaatctt tcctgatcgg tttgaaattc aaaatctggt ttgatatcca
    36661 cctcaacctt tatggagtta aacccttctg aaaactttaa atcttcaacg gtttcattaa
    36721 ttgccgtcct aatatttatt tttctgattc ttagcgcacc cttattattc tgtgaaaaaa
    36781 ccatgatatc gctgattatg ctatccagct tattgattct ttcttcaatc attccgaaat
    36841 attctgaaac cttagattga tccttttcaa gtcttgccac atttaacaat cccaaaaccg
    36901 acgccagcgg tgctcgtaaa tcatgactgg tgctgtaaac cagactgtca agatccttat
    36961 tcgctttttt aagttccctg ctttcgtctt cgagtagcca cactatcatt ccaactccca
    37021 ttaaagattg aattaacaaa tccgctacac ccaggtaaat tgtgaaacca aataaggtat
    37081 gattacccat gtatcccatt ataattgcaa tcgcataatg aaattgctgg agcccataaa
    37141 tcaagaacat ccaaaagaca agtttaacac caagaccacg atgaatcatg gtactccggt
    37201 agatcataaa tgcggctacc ataaaagaaa ctccacttat cagggatcta aacccggccc
    37261 gggtaaggat tcttccgagg gcatttgggt cctgtatatt aacaaaaaca aaaaccaatg
    37321 atgcaacaag aacaagaatt aatgaaagaa caacaatcct attttttacc gctcctttag
    37381 ttgcaacttc gtaggttccc ttcgttagga aaaccaattg aaagtatcca aagaaaaggc
    37441 cgattaatgt tataaaaatt ctaatcccat tatccggagg aattacttca attaaagaat
    37501 aagaaattgc aataccaatt aaatatatcc caaagccaat ccaactgaga caccaggatt
    37561 taaggtattt gtgcctgtaa ctaatataga atctgtaaag gataatcgcg agaattaatg
    37621 cccctgcccc ttgagtcaga taaagtatca atgaatgaat atattcggaa ttggacacca
    37681 ggagtcgatt tttttggaaa acccggggta atattacaat atttttaatt ttaaacgata
    37741 gtgaatttta tgacaggcaa tctctctctc aaattgagga tcttcatttg gctatcgcaa
    37801 tgcataatgg gacctttgcc cgaaaagaat ccacttaaag ccaggagaat ttcaaactcg
    37861 aaacatactg cctgggtcag agatagaagg tggaaggtgg ctaaagttga ggactttgag
    37921 atagaaaatg gattaagaat gagggtttac aagccggaaa tgccaaccaa tggcaaggta
    37981 atttgctatt tccatggagg cggatttgta attggtggcc tggattccca tgataatatt
    38041 tgcagaagat tatgccaatc tgcaagggtt aacctttatt caattgatta tcgccaggga
    38101 cccgaattta aatttccagc tgcaaatgaa gatgcgattg cggcaatcga ttgggttagg
    38161 aagcaagttg ttgatggtgt tgcaacaact gatttattag tggcgggtga tagtgccggt
    38221 ggaaacctgg cagcatttgc ttccctgcat tacccggatt taattcataa gcagatattg
    38281 atttatcccg tccttgattt cattaagccc aaaccatatc accagataaa atattcattc
    38341 tttaactcag aaaatgatgc cagctggtat gcgaaacatt atttaaatga tgaaaaagaa
    38401 aaattagatt ccagattgtc attattaacg cgaaaggatc tggctaaatc tccggagaca
    38461 atgattgtta tcgcggagca tgatattctt aaatttgaag ctattgaatt tgccaagaaa
    38521 ctgatttctc tgggtattaa ggtggatgta aaaaaatatg ccggaatggt tcatggcttt
    38581 cttaactttc cacgatgggc atcaaaagct aaaacaactt ttgaggatat tgcagaattt
    38641 attttgaaaa aagattaatt attttgagaa gagtcaaata aaaatgattg cgactggttt
    38701 aactttcgcg acgaaaagaa atttcttttt gcttatatca actattgcac tgggctctgc
    38761 ttatgggcaa gatgtgccat gggaattcgc tcaggataaa gaggggatag aagtttatac
    38821 ccgacagccg ggaggatcca agttcaagga atttaaaggg ataacggaaa ttaaactttc
    38881 attacacagc gcaattgctt tactcactga tgtaaacacc atgccggaat gggtttcaga
    38941 ttgtgaatcc actaaattga ttgaaaagaa agaaaataaa gttatttacc acatggtaat
    39001 cggagttcct tttccatttg atgacagaga tatggttcag gaacttacat ttatttatga
    39061 caaagaaacg cgtaacatgg aaatcaacct cgggcagaat gatgagataa tggatccctt
    39121 gagaggagtt gtaagaatgc ccgtctctgt tggcaaatgg acctttgaac atttgggaaa
    39181 agaaaagtac cgggtcacct tcgaatattt gtcagatccg ggtggcgcgc tacctgcctg
    39241 gcttgttaat ccctttctta tcaggatgcc atacaaaaca cttttgaatt tcaaagaaag
    39301 agtaaactat cctcaatata aatctgctta ttttgattgg cttggcgcaa caaaataaga
    39361 ttctttttcc ttaatttaca gattaaaatc taatgatgat cgacttaagg agcgacacac
    39421 tgacaaagcc atcaaaggag atgctggaag ccatgatggc
//
LOCUS       DASWHX010000050         7975 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_1512048, whole genome shotgun sequence.
ACCESSION   DASWHX010000050 DASWHX010000000
VERSION     DASWHX010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 7975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7975
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1512048"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(73..1221)
                     /locus_tag="VGA21_03580"
     CDS             complement(73..1221)
                     /locus_tag="VGA21_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009577517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9649617.1"
                     /translation="MTYNRELLSTEIESEDPKILIVDDKPENLVALERLLKNLGVRLY
                     KALSGNEALKLTLHNDFALALLDIQMPGMDGYDLAEILRSDDKTASLPFIFISAIYTD
                     HINIFKGYEKGAFSYITKPFEPKILINKVEFFIEKYRQEHALTKAYTILERKVDELKT
                     LNEELDAYSYSVAHDLKSPLRAISSYSEILYSKVKNESDKEGQQIVETIRNISKGMST
                     LIDDILDLSRLNKFEKKNQNINMGDLFTTVFEELKNNVKKRNIKFVKGDMKPVKGDVR
                     MIRQLVTNLLSNALKYTRTKKTTLIEVGGIEMENEYVYFVEDNGVGFNDKYKNKLFHV
                     FERLHKPSEFEGNGVGLAICKRVVKRHNGRIWAESKLNKGTTFYFSFG"
     gene            complement(1232..4744)
                     /locus_tag="VGA21_03585"
     CDS             complement(1232..4744)
                     /locus_tag="VGA21_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009577516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9649618.1"
                     /translation="MKLNIDTLGKKIGLGFLLLFGVFGVSVTLTILQIRKIEAREKNN
                     IDFEVPSVKHGLIALNGINSSIASLRGWILFNDSKFKEERKKVWQNEIRPSMSRLSTI
                     SSSYWGKTNIDKLASLFSMIEKLESYQEQAEELETAQAEILSEEKIVPLAQEVNTILN
                     QIIDDKEIQMNDALLESEKQIAILSRMQWVLLLSGLLISFISGLIITKSITTPISGAV
                     DVAKDISDGNLETEVNVGGSVELRVLSTALMGMRDSLKSKARESELYSWRTRGQNELY
                     KVMTSYNDVQELANSIISFLSEYISSNIGALYLANDNYTELNLTGKYSFYENEKSQNK
                     FVMGEGVIGQVAITQKKLSIGEMEPKDISIKTSLVQASPKYLFIVPFLFEGKTLGILE
                     FGSVHSFTKEKEEFIESVLESISIAINSAIADQKIKELLEETQQQSEELQTQQEELQQ
                     SNEELEEQTQKLKEQQEELQATNEELEEQSQALEEKNKDLSTAREDIELKAKQLEVGS
                     KYKSEFLANMSHELRTPLNSLLILSNDLLQNRDNNLNEEQLESASVIAKSGYDLLNLI
                     NEILDLSKIEAGKMGLNIEEFRISDLADNLNKEFKVQAREKGLNLEFELDGELPSTIR
                     SDQKRIEQILKNLITNAIKFTHHGTVKISFRSDAGNNLVIQVLDTGIGIPRDKQDVVF
                     EAFQQLEGGTARKYGGTGLGLSISRELSKMLGGRISLESEVNKGSSFTLILPIKFGPV
                     TLKETKDPHMFERSQPEPESNSSFLNYPSIPDDRDNISSDDHVVLIIEDDEKFCTIIA
                     EQARKKGFKYLAASTGEDGLVLTQKFKPHAIILDLKLPGIDGQTVLRTLKGNPDLRHI
                     QIHIISGMDRSLDSVRSGAVQFMVKPVNRRQLSKVFNRIEDFINRKMKSLLILEDDEN
                     LRKSIRKLVGNGDVECLEAGSAREAIDLFMTKTIDCVVLDIGLPDKSGFELIHDLEDR
                     GIHIPPIIVYTGKELSKEENEELRQYSETIIVKGVKSEERLLDETALFLHRTVNNLPK
                     GKQQMIESLYDTEKIFNEKKILVVDDDMRNVFALSKVLRERGMKITKADNGVTALDLL
                     DKDPEIDLVLMDIMMPEMDGYECIENIRRKKRFEALPIIALTAKAMKDDRQKCLDAGA
                     NDYIAKPVDVDKLLSLMRLWIQKL"
     gene            complement(4741..5337)
                     /locus_tag="VGA21_03590"
     CDS             complement(4741..5337)
                     /locus_tag="VGA21_03590"
                     /inference="COORDINATES: protein motif:HMM:NF013502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HEX9649619.1"
                     /translation="MNKVREYDAITIGASAGGMNLVQDMASKLPKDFSMAVILVQHIA
                     NNSDGGWITLLDSTSPLTIKEAEEKEQIERGKIYIAPPNYHLLVENDFTFSLTVDEKV
                     NYARPSIDVFFETASWVFGNRLVGIILSGGNSDGAKGLKSIKDRGGFTIVQDPKSAES
                     PSMPLAAINLVKPHAILNPNEIWEFLLDIHKQIQSDKT"
     gene            complement(5330..6199)
                     /locus_tag="VGA21_03595"
     CDS             complement(5330..6199)
                     /locus_tag="VGA21_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009577515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HEX9649620.1"
                     /translation="MKGNLNSLKVSSKVEPCDIEIELLLEAIFQTYGYDFRDYSRAHI
                     KRRILHRLRLSQIESISLMQHRLLREPGFASDLIRDFSINVTEMFRDPEFYQTLRERI
                     IQILKTWSFLKIWHAGCSTGEEVYSTAILLMEEGLYDRVQIYATDFNTNSLKTAKEGI
                     FPIERIKKYTDNYDLSRPKGRLSDYYLARYDSAIINNSLKKNIIWANHNLVTDHDFTE
                     TNMIMCRNVLIYFNRKLQDKVIALFYSSLVKGGILCLGNKESLINSSFKDYFEEVDKK
                     HKIFKKKYSLSDE"
     gene            complement(6499..6572)
                     /locus_tag="VGA21_03600"
     tRNA            complement(6499..6572)
                     /locus_tag="VGA21_03600"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6536..6538),aa:Arg,seq:tct)
     gene            complement(6728..6802)
                     /locus_tag="VGA21_03605"
     tRNA            complement(6728..6802)
                     /locus_tag="VGA21_03605"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6765..6767),aa:Pro,seq:tgg)
     gene            complement(6834..7211)
                     /locus_tag="VGA21_03610"
     CDS             complement(6834..7211)
                     /locus_tag="VGA21_03610"
                     /inference="COORDINATES: protein motif:HMM:NF017451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HEX9649621.1"
                     /translation="MFSFEKLSVYKRAHLQNIKVLQILTLAVGIDPFLKNQMKRASMS
                     IILNMAEGNGRESWKEKNYFLTVSRGSAFECVAILDTLKALEHINDQQYNELYTEYEE
                     ISRMLIGLKKSLRRSSVQSTTKL"
     gene            complement(7344..7428)
                     /locus_tag="VGA21_03615"
     tRNA            complement(7344..7428)
                     /locus_tag="VGA21_03615"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7392..7394),aa:Ser,seq:gct)
     gene            complement(7516..>7975)
                     /locus_tag="VGA21_03620"
     CDS             complement(7516..>7975)
                     /locus_tag="VGA21_03620"
                     /inference="COORDINATES: protein motif:HMM:NF025420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4249 family protein"
                     /protein_id="HEX9649622.1"
                     /translation="PAPKDCCATCWIEETYNNGVSILSDFNLDGNTVNWPVAFIEDDG
                     GRFMEKYLVNVKQQSLTKEAYQFLNLLNNQLGISGNIFDPPPATIRGNILSIDNPDES
                     TIGYFRVSDISNYSIYVERSVLEEHQKERVINDDCQILRNSFIFPPSYWE"
BASE COUNT         2164 a         1714 c         1328 g         2769 t
ORIGIN      
        1 acaagggcca ggccgacacc tgttccttga aaatttgatt gtttatgaag tctttgaaac
       61 atgcggaata atttatccaa aagagaaata aaaagttgta cccttattga gtttactctc
      121 agcccaaatt cttccattat gtcgctttac gacccttttg caaattgcca gaccaactcc
      181 atttccctcg aactcagaag gtttatgtaa cctttcaaat acatgaaaaa gtttattctt
      241 gtatttatcg ttgaagccca caccattatc ttccacaaag tatacatatt cattttccat
      301 ttcaattcct ccaacttcaa ttaaagtagt ctttttggtt cgcgtatatt ttagggcgtt
      361 tgataataag ttagttacta attggcgaat cattctaacg tcgcctttaa cgggtttcat
      421 gtctccttta acgaatttta tgttcctttt tttgacgtta tttttcaatt cttcaaaaac
      481 tgtggtaaaa agatcaccca tgtttatgtt ttgatttttc ttttcaaact tgttcaacct
      541 tgatagatcg agtatatcat caattagggt tgacattcct tttgaaatat tccttattgt
      601 ttcaacaatt tgttgtcctt ctttatctga ctcatttttg acctttgagt atagtatttc
      661 tgaataggat gaaatggctc ttaaaggaga ttttagatcg tgagccactg aataactgta
      721 ggcgtccagt tcttcattaa gcgttttcag ttcatcgact tttctttcca gaattgtata
      781 ggcctttgtc aacgcgtgtt cttgtctgta tttctcaatg aagaattcta ctttgttaat
      841 taggattttg ggctcgaaag gttttgttat ataactaaaa gctccttttt catatccctt
      901 aaaaatatta atatggtcag tgtaaattgc cgaaataaat atgaaaggca acgatgcagt
      961 tttatcgtcc gatcttaata tttctgccaa atcatatcca tccattcccg gcatttgaat
     1021 atcgagtaat gcaagtgcaa aatcattatg aagtgttagc ttaagtgctt cattgccact
     1081 aagcgctttg tataacctta cgccaagatt tttgagtagt ctttcaaggg ctaccagatt
     1141 ctcaggctta tcatccacaa taagtatttt cggatcctcc gattcgattt cagttgaaag
     1201 taactcccta ttataagtca ttagcatttc cttacaattt ctgaatccat agtctcatta
     1261 gtgacaataa cttatcaacg tccacaggct ttgctatgta gtcattggct cctgcatcaa
     1321 ggcatttttg acgatcatcc ttcatggctt tagcagttaa agcaattatc ggcaaggcct
     1381 caaaacgttt tttccttcga atgttttcta tgcattcgta gccatccatt tcaggcatca
     1441 taatgtccat caataccaaa tctatttcgg gatccttgtc aagcaaatcc aatgcagtca
     1501 ccccgttatc tgcctttgtg attttcattc cacgctccct gagaactttg gaaagggcaa
     1561 agacattgcg catatcatca tccacaacaa gaattttctt ctcattaaat attttctcag
     1621 tatcgtataa actttcaatc atttgctgtt tccctttcgg gagattgttc acagtcctgt
     1681 gcagaaataa agcagtctca tccaacagtc tctcctctga ttttacccct tttacaatta
     1741 ttgtctcaga atattgacgc aattcttcgt tttcctcttt agaaagttcc ttccccgtat
     1801 atactataat tggtggtatg tgaattcctc tatcctcaag atcatgaatt aattcaaatc
     1861 cacttttgtc tggtaatcct atatcaagaa ctacacaatc aattgtcttt gtcataaaaa
     1921 ggtcgattgc ttccctggca ctgccggctt caagacattc cacatcacca tttccaacca
     1981 attttcttat tgatttccga agattctcat catcttcaag tattaacaaa ctcttcattt
     2041 ttcgattaat gaaatcttca atacggttaa aaactttaga taattgccgg cgattaacgg
     2101 gttttaccat aaattgtaca gcgcctgatc taacagaatc caaagatcta tccattccag
     2161 aaataatatg tatttgaata tgcctcaaat ccggattacc ctttagtgtt ctcagtactg
     2221 tttgcccatc aattccaggc aattttaggt caagtataat tgcatggggt ttaaattttt
     2281 gtgtgaggac cagaccgtct tcacctgtgg atgctgctaa gtacttaaat ccttttttcc
     2341 tcgcctgttc agcgattatt gtacagaatt tttcatcatc ttcaatgata agaactacat
     2401 gatcatcgga actaatatta tcgcgatcgt ccggtatgct tggataatta agaaaacttg
     2461 aattagactc aggttcaggt tggctccttt caaacatgtg cgggtccttt gtttccttta
     2521 aggtgacggg accaaatttg atcggcaaaa tcaaggtgaa agatgaacct ttgtttactt
     2581 cactttcaag gcttatcctt cctccaagca ttttcgacaa ttcccttgaa atggataatc
     2641 ctaatccagt tcctccatat tttctggcag taccgccttc caattgctga aatgcttcaa
     2701 aaactacatc ttgtttatct cttggaattc caatccccgt atccagaact tgtataacta
     2761 aattattacc ggcatcactt ctaaaggata tttttactgt cccatggtgc gtgaatttaa
     2821 tagcattagt tataaggttt ttgagaattt gttcaatcct tttttgatct gagcgtattg
     2881 ttgagggaag ctcaccatca agttcaaatt ctaaattgag tcccttttcc cttgcctgaa
     2941 ctttaaattc tttatttaga ttgtctgcca gatccgaaat tcggaattct tctatattca
     3001 aacccatttt ccctgcttca attttagaca gatccaaaat ttcatttatt aagttgagta
     3061 aatcgtaacc actttttgca atgacggaag cactttccag ttgctcctcg tttagattat
     3121 tgtccctgtt ttgtaaaagg tcgtttgata aaatcaaaag actattaaga ggcgtgcgta
     3181 attcatggct catattagca agaaactcgg atttgtactt actaccaact tcaagctgtt
     3241 tagctttcag ttcaatatct tcgcgcgctg ttgagaggtc tttgtttttt tcttcaagag
     3301 cctgagattg ttcctcaagc tcttcatttg ttgcctgtaa ttcctcttgc tgttccttca
     3361 gcttttgcgt ttgctcctct aactcttcat tggattgttg tagctcttcc tgctgggtct
     3421 gtaattcttc gctttgctgt tgtgtttctt caagcaattc tttgatcttc tggtccgcaa
     3481 ttgccgaatt aattgctatt gaaatacttt ccaatactga ttcaataaat tcctcctttt
     3541 ccttcgtaaa agagtgcaca ctgccgaatt ccaggattcc caatgttttc ccctcaaata
     3601 agaatggaac tatgaatagg tattttgggc tagcttgaac aagggaagtc ttaatgctaa
     3661 tatctttcgg ttccatttct cctattgaaa gttttttttg ggtaattgct acttgcccta
     3721 taactccttc gcccatcaca aatttattct gggatttttc attttcgtag aaggaatact
     3781 ttccggttaa gtttagttca gtatagttgt cattcgccaa atacaaagca cctatattgg
     3841 aagatatata ttcagacaaa aatgatatga tgctgtttgc cagttcttgg acgtcattat
     3901 agctggtcat tactttgtaa agctcattct gccctcgtgt tctccaacta taaagctcgg
     3961 attccctggc tttcgatttg agggaatccc gcattcccat tagtgctgta cttagtacac
     4021 gtagctccac tgaacctccg acatttactt cggtctccag atttccatct gaaatatctt
     4081 tcgcaacatc caccgcacca gatatcggtg ttgttataga tttcgttata atcagacctg
     4141 aaataaaaga tattaagagc ccggacaaca gcagcaccca ttgcattctt gacagaattg
     4201 caatctgctt ttcagactcc aacaatgcat cattcatctg aatttcctta tcgtcaatga
     4261 tctggttaag aattgtgttt acttcttggg caaggggtac tattttttct tctgataaga
     4321 tctctgcctg ggccgtttct aattcctccg cttgttcctg gtagctttct aatttttcta
     4381 tcatcgaaaa aagtgaggct aatttgtcaa tatttgtttt accccagtat gaagaagaga
     4441 tggtgcttaa tctgctcatc gaaggacgaa tctcattttg ccataccttt ttcctctctt
     4501 ccttgaattt tgagtcgtta aacagaatcc agccccggag ggaagcgatt gagctattaa
     4561 taccgtttag tgcgattaac ccatgcttaa cagaaggaac ttcgaaatct atgttatttt
     4621 tttctctcgc ttcgattttt cttatctgca aaatggtcag ggtgacagac actccgaaca
     4681 ctccgaacaa aagcaaaaat cccaagccta ttttttttcc taaagtatct atgttaagtt
     4741 tcatgttttg tcggattgaa tttgtttgtg aatatctaaa aggaattccc atatttcatt
     4801 gggatttaga atagcatgag gttttaccag attgatagca gctaatggca ttgaagggga
     4861 ttctgctgac tttggatcct gaacgattgt aaaccctcct ctgtctttta ttgattttag
     4921 cccttttgcc ccatcggaat ttccgccact caatatgatc cctaccagtc tattaccgaa
     4981 tacccaactt gccgtttcaa aaaatacatc aatggatggc ctcgcataat taaccttctc
     5041 atctacggta agggaaaatg taaaatcatt ttcgactagc aaatggtaat ttggaggagc
     5101 tatataaatc ttcccccttt caatttgttc tttttcttct gcttccttta tggttaacgg
     5161 actcgtgcta tccagcaaag ttatccatcc gccgtctgaa ttatttgcaa tatgctgtac
     5221 aagtattaca gccatggaaa agtcttttgg taattttgat gccatgtcct ggacaaggtt
     5281 catcccgccg gcagaagctc caatagttat tgcatcatat tccctaactt tattcatcgc
     5341 ttaaagaata tttcttctta aaaattttat gctttttatc cacttcttca aagtagtctt
     5401 taaagcttga attgatcaaa ctctctttgt tgccaagaca aaggatccct ccttttacca
     5461 agcttgaata aaacaaggct attaccttat cctgcaattt cctgttaaaa tagatcaaca
     5521 catttctgca cattatcata ttagtttcgg tgaaatcatg atcagtcacc aggttatgat
     5581 tagcccatat aatgtttttc ttaagggaat tatttatgat agcagaatca tatcttgcca
     5641 ggtaataatc tgataacctt cccttgggcc tggagagatc ataattatct gtgtatttct
     5701 taattctttc tatcgggaat attccctcct tagccgtttt taaggagttt gtgttaaaat
     5761 ccgttgcata gatttgaact ctgtcgtaca atccctcctc cataagtaat atagcggttg
     5821 aatatacctc ttctcctgtg gaacatccgg cgtgccatat cttaaggaat gaccatgttt
     5881 taagaatctg gattatcctt tccctcaacg tttgataaaa ttccggatcc cggaacattt
     5941 cagtaacatt gattgaaaag tccctaatta agtcggatgc aaaacctggc tcgcgaagca
     6001 gcctatgttg catcagcgaa atgctttcaa tttgggacaa ccgcagccga tggagaatcc
     6061 ttcgtttgat gtgagccctg gaataatccc tgaaatcata tccatacgtt tgaaaaattg
     6121 cttcgaggag aagttcaatt tcaatgtcgc agggttctac cttagaagat acctttaaac
     6181 tgttaagatt tcctttcact taaatttggt tctttaagga gtatatctca aaccaagctt
     6241 taaggaaata gtttgtggta agtcttacag gtagtaattt aacgcttttt ttggcaaaag
     6301 atatgcttcg atgtgtattt ttagtgaagc tttaacccgt taaatccact gatttttttt
     6361 agtaatcggg taattattcc cctccacctg aaattagtcg gactgggggt tgcttgctgg
     6421 attgcttatt tcttgcgtta ttccgcaagg cttcctgctc ttcaaaaaga aaaataattt
     6481 tctcttcttt tttctctgtg attccgccgc gattcgaacg cggaacctgc tgcttagaag
     6541 gcagctgctc tatccagttg agctacggaa ccaaagtgag tgtgcgtgtt tagagttaag
     6601 ttttaaaagt ttatggttta gggggtttag ggtttagagg ttagagggca ttaaaacctt
     6661 aactcttaac tcttaacttc taactcctaa ctcccaactc ttaactccta actcctaact
     6721 cccattgtcg gggtggcagg attcgaacct gcggccccct gctcccaaag caggtgcgct
     6781 aaccggactg cgctacaccc cgtagttgaa gttatgtgtt aagagtttag agttcatagt
     6841 tttgttgttg actgcacgct actccttctt aaacttttct tgaggccaat tagcatcctg
     6901 gatatttctt catattcagt atacaattcg ttgtattgct gatcattaat atgttccagc
     6961 gctttcagag tatccagaat tgctacgcat tcgaaagccg aacccctaga taccgtcagg
     7021 aaataattct tttctttcca cgattcccga ccatttccct cggccatatt caaaattata
     7081 ctcatacttg ccctcttcat ctggttcttc aaaaaaggat caatccccac tgccaaggta
     7141 agaatttgca acactttgat attttgtaag tgcgccctct tatatacact taacttctca
     7201 aaactaaaca tcaaacaaag agcgattcgt caaaataaaa agtttttagt gtcttaattc
     7261 gcagcactta actcttaact cctaactcct aacccttaac tcttaactct taactcctaa
     7321 ctcttaactc ttaactcaac tagcggagag agggggattc gaacccccgg tacctaatgg
     7381 tacggcagtt tagcaaactg ctggtttcag cctctcaccc acctctcctg gttaaaaacg
     7441 actgcaaata taataaaccc tgatcgacct tgaacaatct ttgaatttta attaaatgaa
     7501 cacccggtaa aaaaattatt cccagtagct tggcgggaag ataaatgagt ttctcaaaat
     7561 ttggcaatca tcgttgataa cgcgctcttt ttgatgctcc tccaatactg accgctccac
     7621 atatatggag taattggata tatccgacac cctgaaatat ccgatagtac tttcatccgg
     7681 attatcaatg ctcaggatat tcccccgaat tgttgccggt ggcgggtcaa aaatatttcc
     7741 tgaaataccc aactgattat ttagcaaatt gagaaactgg tatgcttctt tagttagtga
     7801 ttgctgttta acattaacaa ggtatttttc catgaatctt ccgccatcat cctcaataaa
     7861 cgcgacaggc cagttcaccg tatttccatc aaggttaaaa tcagacaaaa tgcttacccc
     7921 attgttatag gtttcctcta tccagcatgt cgcgcagcaa tctttaggag caggc
//
LOCUS       DASWHX010000051         7836 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_779860, whole genome shotgun sequence.
ACCESSION   DASWHX010000051 DASWHX010000000
VERSION     DASWHX010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 7836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7836
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_779860"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            656..1447
                     /locus_tag="VGA21_03625"
     CDS             656..1447
                     /locus_tag="VGA21_03625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649623.1"
                     /translation="MFKAIVPASQVIVDGPIVLKKSKEKLRLLKSQPKRKIEGPERKL
                     DSKSLGFGKIDFQTLQIRFTNALFEFWSNPRLRVGLVFVLVIAPLSKFSYLIFPKEGF
                     GLYLVNNEFFSIPNLIEGTHWYFRSLFYFFFSINEFLTPLLTIIGIYFLFPKNYYPSY
                     LCGVPAGYYFALLIKRITVNSDLGFHSPVALSIVLSAILFTVFLFYMSDKMLFKTNHR
                     KRAIEARILGLINMPGMSWKDKEALLKREAKDAMKANNELFNRAG"
     gene            complement(1450..1800)
                     /locus_tag="VGA21_03630"
     CDS             complement(1450..1800)
                     /locus_tag="VGA21_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013452901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEX9649624.1"
                     /translation="MKFIIKLLLTALAVLLISYLLPGVHTDDYGVAVLVALLLAILNV
                     TVKPLIILLTIPITFLTLGLFLLVINAVIILLADSIIDGFYVNGFWWALLFSLILSLL
                     VSFFDKELNKENRR"
     gene            1902..2600
                     /locus_tag="VGA21_03635"
     CDS             1902..2600
                     /locus_tag="VGA21_03635"
                     /inference="COORDINATES: protein motif:HMM:NF022441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HEX9649625.1"
                     /translation="MKRLLLPIFVLIAFAAISQGRGDFTREVSEFRKIKAGNGVKVNL
                     LQGKSGQITIHSHGIAQEDIITEVNGKTLKLSIDAWSQLREKNKRWEVEIDVPVEKLD
                     EITSVTGARVVGDFKLSGRKILITGNTGGELYLDLEMEEVIVNASTGSILKLKGQTDY
                     LEVTSNMGSEVEAYTLHANYVLAKASLGGTLEVYARNEINSNASFGGVIEVTGNPSRT
                     HTRKTLGGEVSGVR"
     gene            complement(2632..3174)
                     /locus_tag="VGA21_03640"
     CDS             complement(2632..3174)
                     /locus_tag="VGA21_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009582006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEX9649626.1"
                     /translation="MTREEARELLHSFTESESLLRHARSVELVMEAYAKKLNEDTEEW
                     AIAGMLHDADYEKYPDQHPNIIVSKLKQIGEANLAHAISAHYTKWGVKYETLLDKALL
                     ACDELTGFIIACSHVRPDKIEGLQSKSVIKKLKDKSFAAKVEREEVQAGAELLGVELP
                     DHIDFIISVLQENKKELNLI"
     gene            complement(3171..4337)
                     /locus_tag="VGA21_03645"
     CDS             complement(3171..4337)
                     /locus_tag="VGA21_03645"
                     /inference="COORDINATES: protein motif:HMM:NF026288.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9649627.1"
                     /translation="MQRNAGIILSIGLALIFSNGMGQTDSLENALQNTTGAAKVKTLN
                     ELYKVFINSEPDKALKYTVDAFELAKEIEDERGIATCYNNMGVFYKNQGTLDKALDYY
                     LKALSLGQTIHDKDLIANLYNNIGTIYSIKKEYRQAEDYYKRSYNILDSLGNKINMVG
                     SLNNLGNVYAETGRSSEAIEYYRQAVEIYSESGQTRNSHEPLTSIGNVHFRNADYDKA
                     LEYFNQAFEIEKENNNLFGQARALHDMGLTHLRKENYGEALLTVESALEIADQLGAVP
                     ILEVIYKALAEIYYTTGDIKRAYESIILYEQMRDLQFNETTSRKLAELDVAYEFERLE
                     KEIEELRQENEINSLRAENSRVVILVGIMGTLIIVGALFVYYATRKKTKKKATT"
     gene            complement(4406..5212)
                     /locus_tag="VGA21_03650"
     CDS             complement(4406..5212)
                     /locus_tag="VGA21_03650"
                     /inference="COORDINATES: protein motif:HMM:NF024141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9649628.1"
                     /translation="MIRLFNIEWIKLKNYKTFWVLGGLYLFLITFIPISVLEFMKWLE
                     SKGVEFEEFSPSKIPILHFPDIWQNITYLSVFFKFFIAVIIIISITNEFSYKTIRQNI
                     IDGLSRMDFLKSKLTAIVAICLISAVYLFLICLITGLIYTPRLIQGEILEYSQFIFAY
                     FLDILFFLIFALLIGNTFKKAGISIALVVMIIPIEYIFSANLPDEISVVGDYLPMHVM
                     NSIIQLPFTKYGFMEIQDYISPEAVALSFAYIALFVYLNYVLLKKRDLTS"
     gene            complement(5209..6105)
                     /locus_tag="VGA21_03655"
     CDS             complement(5209..6105)
                     /locus_tag="VGA21_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013453516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9649629.1"
                     /translation="MPDYILELENLSKRYGRIKAVDELYLKVEKGNIFGILGPNGSGK
                     TTTLGVVLDVVNRTGGNFKWFGEEPTTAHRKRIGAILETPVFYPYLSGYKNLQLIAQI
                     KEVPNEKIEPVIAKVNLADRQHDKFRTYSLGMKQRLAIAAALLCDPEVMILDEPTNGL
                     DPQGIADIRQLIIQIGKEGKTIILASHLLDEVQKVCTHFAVLKNGKKLFQGSVEDINK
                     GMNEVEIKSEDSRILELLKNFNGVKTVIQEGNTYTIKLNDGLSSTDINKYLFENGIVA
                     SHLVTLRKSLEKQFLEILEESE"
     gene            complement(6107..6583)
                     /locus_tag="VGA21_03660"
     CDS             complement(6107..6583)
                     /locus_tag="VGA21_03660"
                     /inference="COORDINATES: protein motif:HMM:NF025426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4252 domain-containing protein"
                     /protein_id="HEX9649630.1"
                     /translation="MIRSLLLSSALLLAMPSVKAQTKETNDFHHAHPDAFTLFFYKST
                     IRMLDPTNSDEYFNMIKDIERAKFLRFNESEISDSDIQNYIASIKEVSFEEGMTMRQG
                     AGTANVFIRDAVSEKSGIIFIMKADDQVFILDIVGKVQVSKLMELTKKINIFESLF"
     gene            complement(6688..7218)
                     /locus_tag="VGA21_03665"
     CDS             complement(6688..7218)
                     /locus_tag="VGA21_03665"
                     /inference="COORDINATES: protein motif:HMM:NF025426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4252 domain-containing protein"
                     /protein_id="HEX9649631.1"
                     /translation="MKRIILFFMVMGFAGVLKAQNDAIDRFFSKYIDDPEFTYAVVTG
                     KMFGLFTHMEGESENDKALLDAISSVKGLKVLAIEDTERGTALYTEAFTIIPAREYEE
                     LMTVRDEDGNMKFLIKEAKGIISELLMIRGATHDFMIVSLVGNIDLKSISKLARHVNI
                     DGFEHFEKMEKKEEKH"
     gene            complement(7232..7765)
                     /locus_tag="VGA21_03670"
     CDS             complement(7232..7765)
                     /locus_tag="VGA21_03670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649632.1"
                     /translation="MDSKRIETLLDRYWSCVSTLEEEEELKKFFNSDEVPERFKEYEN
                     LFKYFEAEKKSSGLGDSFDRELAQRIRLLKPSNSSGKIFFNYLKVAAVIAMVLVASFL
                     FRQNLAPENRPDLMGTYEDPQEAYEETKRVLMLVASKLNKGRKYTETIGAFNEAEEKI
                     KGGEDETDNNENKDIQL"
BASE COUNT         2325 a         1519 c         1473 g         2519 t
ORIGIN      
        1 ttatgtgatt ttggttttac ttgtttgctt gaattaggat acaaaatgct gcaggaagaa
       61 aaggaaagcg gcaggcatat tagagttatt aagaacagat taagaataag agactttcgt
      121 ttcatctcag cttatttgat agagtaatag tacgattttg gtcaacttca aataaaaaac
      181 ttacaatgca tagagtaatg tgaaaaacta tgtctaattt caatgatgat tatcaatttc
      241 gtggtgatta tttaattaaa aatattaaat taaacccagc acagtgttga actaatcaaa
      301 tatatgataa gaggagtaat acttatttga gaagtattaa taggattaca ataatagtca
      361 ccacatgtta ttattatcta ttaaactgaa agaattgaat ttattgaagt aaccttctct
      421 cattatttgt aatttatgtg aatccttaga actttaaata tataatagtt acaagcccat
      481 gaaaccatca aaatttgtaa atgaactggt cattggtatc ttcggggaaa attttttcac
      541 ccgaaaacca aatttatcat gcgaacaaga gcaatcaaaa gaatcatttt ttcggcactc
      601 attattggcg ccggtacctt cttatgggca aaagagccaa gaaataagcc aacagatgtt
      661 caaagctatt gtccctgctt cccaggttat tgtggatggc ccgattgtgt taaagaaaag
      721 caaggagaaa ttgcgtttgt tgaagtctca gccaaaacgg aagattgaag gtcctgagag
      781 gaaactagat tctaaaagtt tagggtttgg aaaaattgat ttccagaccc ttcaaattcg
      841 ctttaccaac gccttattcg aattttggtc aaatccaaga ctaagagttg gtttagtatt
      901 tgtacttgta attgcacctc tttcaaagtt ctcttacctt atttttccga aagaaggctt
      961 tgggctttac ttagtaaata atgaattctt ttctattcca aatttgattg aaggtactca
     1021 ctggtacttt aggtctttat tttatttttt cttttctata aatgaatttt taaccccatt
     1081 gcttaccatt attggcatct attttctatt tccaaagaat tactatccat cctatttatg
     1141 tggtgttcct gctggttatt atttcgcact tcttattaaa agaattactg tgaattcaga
     1201 ccttggattt cattcaccag ttgccctctc aattgttctt agtgcaattc tcttcacggt
     1261 ttttcttttc tacatgagtg ataagatgct attcaaaaca aatcatagaa aacgagccat
     1321 cgaagccaga attcttggct taattaatat gcctggaatg tcatggaaag ataaggaagc
     1381 actactgaag agggaggcaa aggatgccat gaaagcaaac aatgaattgt tcaacagggc
     1441 aggatagtac tatcttctgt tttccttgtt tagctcttta tcaaaaaagg aaacaagcag
     1501 agagagaatc aggctgaata acaaagccca ccaaaatccg tttacataaa atccgtcaat
     1561 tatcgaatcg gctaataaga taattactgc atttatgact aataaaaata atccaagtgt
     1621 caaaaaagtt atagggattg tcagaagaat aatcaaaggt tttactgtaa cattcagaat
     1681 tgccagtaag agggccacca acactgcaac gccataatca tccgtgtgaa cccccggcag
     1741 cagataggaa ataagtaaaa cagccagggc cgtaagtaag agtttgataa tgaatttcat
     1801 atgctaaaat tatcaaaaat atcggggcat aatgcaacta attagctttc attttagtct
     1861 tataaagagt aaggttcaat tcatcattta aaaacctgat tatgaaaaga ttattattac
     1921 ctattttcgt gcttatcgct ttcgcggcaa tttctcaggg aaggggtgac tttacccgtg
     1981 aggtaagcga gttcaggaaa ataaaagccg gcaatggagt gaaggtaaat ctactgcagg
     2041 gaaaatccgg ccaaataacc attcattcgc atggcattgc tcaggaggat atcataactg
     2101 aagtcaatgg taagacatta aagctaagta tcgatgcctg gtcacaattg agggagaaaa
     2161 ataagagatg ggaagttgag attgatgttc cggtggaaaa gcttgacgag ataacttctg
     2221 ttacaggtgc ccgggtggtt ggggacttca aattgtccgg gaggaaaatt ctcataaccg
     2281 gtaataccgg tggcgagctt tacctggatc ttgaaatgga agaagtgatt gttaatgcat
     2341 ctaccgggtc cattcttaag ttaaaaggac aaaccgacta tcttgaagtt acctctaaca
     2401 tgggttctga agtagaggct tatacgcttc atgcaaatta tgttctggcg aaagcgagct
     2461 tgggagggac tctggaagtt tacgccagga atgaaatcaa ctcaaatgca tcgtttggcg
     2521 gggtaattga agtaaccggt aatccttccc ggacacacac gcggaaaact ctgggtggtg
     2581 aagtgagtgg tgtccgataa atgatttgag ttactcacag ggaagagcta gtcaaattag
     2641 gttcagctct tttttatttt cctgaagtac agaaataata aagtcaatat ggtctggtag
     2701 ttctacccct aacaattcag ctcctgcctg cacttcttcc cgttcaactt tggcagcgaa
     2761 acttttatcc ttcaatttct ttataactga tttcgactga agaccttcga ttttatcagg
     2821 tcttacatgt gaacatgcga taataaatcc tgtaagttca tcacaggcaa gcaaagcttt
     2881 gtccagcaaa gtttcgtact taactcccca ttttgtgtaa tgcgcggaga ttgcatgggc
     2941 aaggttcgct tcacctattt gcttcaattt actcacgata atgtttggat gttggtcggg
     3001 atatttttca taatcggcat catgcagcat tccggcaatt gcccattctt cagtatcttc
     3061 atttaacttt ttcgcgtagg cttccatcac cagttcaaca cttcgtgcat gcctgagcaa
     3121 actttcggat tccgtaaagg aatggagtaa ttcgcgtgcc tcctcacgag tcatgtagta
     3181 gctttctttt tcgttttctt tctggttgcg taatagacaa aaagggcgcc aacaataatg
     3241 agcgtaccca taataccaac aagtattact acacggctat tttcagcccg caaagagttt
     3301 atttcatttt cctgcctgag ctcctcaatc tctttttcca acctttcgaa ttcataggca
     3361 acatcaagct ctgccagttt cctgctggta gtttcattga attgcagatc acgcatttgt
     3421 tcgtagagaa tgatgctttc gtaagcgcgt tttatatcac ctgtggtgta atatatctct
     3481 gctaacgctt tgtatatcac ttcaagaata ggtactgcgc ccagctggtc agcaatttcc
     3541 aaagcagatt cgacggtaag taatgcctcc ccgtaatttt cttttctcag atgggttaaa
     3601 cccatatcat ggagcgcacg cgcttgccca aataaattgt tattctcttt ttcaatttcg
     3661 aatgcctgat tgaaatactc cagggcttta tcgtaatccg cgtttctaaa gtgaacattt
     3721 cctatgctgg ttaagggttc atgagaattc ctggtttgtc cgctttcaga ataaatttca
     3781 actgcctgac gatagtattc aatagcttcc gaacttcttc cggtttcggc atatacatta
     3841 ccgagattat ttaatgatcc aaccatgtta atcttgtttc ccaagctgtc taagatgttg
     3901 tacgatcttt tgtaataatc ttcggcttgc ctgtattctt tttttattga ataaatggtt
     3961 ccgatattat tgtaaagatt agcgatgaga tccttgtcat gaattgtttg tccaaggctt
     4021 aatgctttca aataataatc aagagcttta tcaagcgtgc cctgattctt gtagaaaacg
     4081 cccatattgt tatagcaggt ggcaatgccg cgttcatcct caatttcctt ggcgagctca
     4141 aaggcatcaa ctgtatattt caatgctttg tcgggctcag aattgataaa aactttatat
     4201 aattcgttca gggttttgac ttttgcagct ccggttgtgt tttgaagggc attctccaga
     4261 ctatcagttt gccccatacc attagaaaat atcaaggcaa gtccaatgga gagtattatt
     4321 cctgcatttc tttgcataaa ggttgatatt acagtttcct ctaaaattag cgggtttgca
     4381 ataagaaaaa aaggaatttg gtaattcagc tggtaaggtc tctctttttc agcagaacat
     4441 agttcaaata cacaaataaa gcaatgtagg cgaaagacaa agcaaccgcc tccgggctaa
     4501 tataatcctg aatttccatg aaaccatact tggtgaacgg cagttggatt atactattca
     4561 tcacgtgcat aggcaggtaa tctccaacaa ctgagatttc gtctggaaga ttggcggaga
     4621 aaatatattc aatgggaata atcattacga ctaaagcaat tgagatcccg gccttcttaa
     4681 aagtattgcc aatcaaaagg gcaaaaatca gaaagaacag aatgtccaga aaataggcga
     4741 aaataaactg gctgtattca agaatctcgc cctggataag cctgggtgtg taaattaatc
     4801 ctgttatcag gcatatcaag aaaagataaa cggcgctaat caggcatata gctactattg
     4861 cggtgagttt tgatttcaaa aaatccattc tgctcaagcc atcaataata ttttgtcgga
     4921 tagtcttgta actaaactca ttggttatcg agataataat aataaccgcg atgaaaaact
     4981 taaagaagac tgacaggtag gtaatatttt gccaaatatc cggaaaatgc agaataggta
     5041 tttttgaggg cgaaaattct tcaaattcaa cgcctttgct ttccagccat ttcataaact
     5101 ccagcacact aattggaata aaagttataa ggaatagata gagcccaccc aatacccaaa
     5161 aggttttata gttttttagc ttaatccatt cgatgttgaa aagtctaatc attctgattc
     5221 ttctaaaatt tccagaaact gtttctcaag agacttcctt aatgtaacca ggtgagaagc
     5281 aacaattcca ttttcaaaca aatatttatt gatatcggta gaggaaagcc cgtcatttag
     5341 tttaatggtg taggtattgc cttcctgaat cacagtttta accccattaa aatttttcag
     5401 caattcaaga atcctggaat cttcggattt aatttcaact tcgttcattc ctttgttaat
     5461 atcctccacc gatccctgga agagtttctt tccattctta agaactgcaa agtgtgtgca
     5521 aactttttga acttcatcaa gaaggtgact tgccaaaata attgttttgc cttcttttcc
     5581 gatttgaatt attagctggc ggatatcggc gataccctgc gggtccaagc cattagtagg
     5641 ttcatccagt atcatcactt ccggatcgca aagcaatgcc gcggcaattg ccagtcgttg
     5701 tttcatacca agagaatatg tccggaattt gtcatgttga cggtcggcca ggtttacttt
     5761 ggcaattaca ggctctattt tttcgttggg aacttcctta atctgagcaa tcagctggag
     5821 atttttataa ccggagaggt atgggtaaaa aaccggagtc tcgaggattg caccaattcg
     5881 ctttctgtga gctgttgttg gttcttctcc aaaccatttg aaatttccac cggttctgtt
     5941 tactacgtca agaacaacac caagggttgt agttttacca ctcccgttag ggccaaggat
     6001 gccaaagata tttccttttt ccaccttgag gtaaagttcg tcaacggctt ttatcctgcc
     6061 gtagcgtttg gagagatttt caagttcaag aatataatcc ggcattttaa aatagtgatt
     6121 cgaaaatgtt aatttttttg gtaagttcca tcaattttga aacctgcact ttcccaacaa
     6181 tatcaagaat gaacacctgg tcgtccgctt tcatgataaa gataattccg cttttttcac
     6241 taacggcatc cctgatgaag acattagcgg ttccagctcc ctgtcgcatt gtcattcctt
     6301 cttcaaaaga tacttcctta atgctggcaa tataattctg aatatctgaa tcagagattt
     6361 cagattcatt aaatctcagg aacttagcgc gctcaatgtc tttgatcatg ttaaagtatt
     6421 catcggaatt ggtaggatcc agcatcctga ttgtgctttt gtaaaaaaac aacgtgaatg
     6481 catccggatg tgcatggtga aaatcattgg tttccttggt ctgagcctta acagatggca
     6541 ttgccagtaa caatgcagaa ctgaggagta gcgagcgtat caattcctgt aaatttttga
     6601 cctgaaagtt aggaaaattt tagctattgg atggaggatt ggaaaaaatg aggagcattg
     6661 gtaaatgctc ctcataactg gttattttca gtgtttctct tcttttttct ccattttttc
     6721 aaagtgctcg aacccgtcaa tattcacgtg tcgtgccaat ttagaaatgg atttcagatc
     6781 gatatttccc acaaggctta caatcataaa atcgtgagtg gcacctcgta tcatgagcag
     6841 ttcactgatt attccctttg cttcttttat cagaaacttc atgtttccat cttcatcgcg
     6901 aacagtcatt agctcctcgt attccctggc tgggattatt gtaaatgctt ctgtatataa
     6961 agctgtgcct ctttcagtat cttcaatggc aagaacctta agtcctttca cagaactgat
     7021 ggcatcaagc aatgccttat cattttctga ttcaccttcc atgtgagtaa ataaaccaaa
     7081 cattttcccg gttaccaccg cataggtgaa ctcgggatcg tcgatatatt tgctgaagaa
     7141 tcgatcaatt gcatcatttt gagctttcag cactcccgca aagcccataa ccataaaaaa
     7201 caaaattatc cttttcattt ttgaatttaa tttaaagttg tatgtcctta ttttcattgt
     7261 tgtctgtttc atcttcacct ccttttatct tttcctcggc ttcattgaac gccccgattg
     7321 tttcagtata ttttcgtccc ttgtttaact tggaagccac caacatcaat actcttttgg
     7381 tttcttcgta tgcttcctga gggtcctcat aggtgcccat tagatcaggt ctgttctccg
     7441 gtgccaggtt ttgcctgaac aagaaggaag caacaagtac catcgcaata acagcagcca
     7501 ctttcaaata attaaaaaag atttttcccg aactgttgga aggtttcagc agtcttattc
     7561 tttgcgcaag ctctctgtca aatgaatctc ccaggcctga agattttttt tcggcctcaa
     7621 aatatttgaa taagttttcg tactctttaa atctttcagg tacttcatca gagttgaaaa
     7681 actttttcaa ctcttcttcc tcttccagtg tagaaacaca ggaccagtac cggtccagta
     7741 acgtttcaat tcttttagag tccatatgaa ttaatgttaa gtaggttctc tttaatactt
     7801 ttccttgccc gaaacagatt cactttaacc tggttg
//
LOCUS       DASWHX010000052         7603 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_2620436, whole genome shotgun sequence.
ACCESSION   DASWHX010000052 DASWHX010000000
VERSION     DASWHX010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 7603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7603
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2620436"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            86..156
                     /locus_tag="VGA21_03675"
     tRNA            86..156
                     /locus_tag="VGA21_03675"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:118..120,aa:Cys,seq:gca)
     gene            435..920
                     /locus_tag="VGA21_03680"
     CDS             435..920
                     /locus_tag="VGA21_03680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649633.1"
                     /translation="MNKNNPKLYHHNVFVTIIFTGLLAGLLDGAAAVIHVTILGSTQP
                     MKVFNYIASGIFGDAAFSGGIAMTYFGIFFHLGIATIWTALFFLFYKVFIKVTGNWLI
                     LGILYGIIVWMGMNLVVLPLSNTPPRTFSINNTMIGVLILITCVGIPVAYSSFKFQNK
                     R"
     gene            986..1837
                     /locus_tag="VGA21_03685"
     CDS             986..1837
                     /locus_tag="VGA21_03685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649634.1"
                     /translation="MKICFATSEKTLSLTPSDRSLFEAMQRNDMEVSVSAWSDRKINW
                     SEFDVIIIRSCWDYHLRIGQFNSWLNELDRLKIKVFNPIEVLRGNLDKKYLGDLQEKG
                     VRIPTTLWFEAEAKLDCKDILRSQGWQEAVLKPNIGATAYGVRKISLKTSENLFIDNN
                     PEFLNGFMVQKFIPEIETQGEWSIIFFGKEYSHSVIKKTNRGEFRIQYEYGGSFKPAN
                     PAQGVISEARSILKYYPDNLLYARIDGVLAGNKFYLMEAELTEPELFISERVFADRFV
                     SRMRELL"
     gene            complement(1970..2947)
                     /locus_tag="VGA21_03690"
     CDS             complement(1970..2947)
                     /locus_tag="VGA21_03690"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009195066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex E1 component
                     subunit beta"
                     /protein_id="HEX9649635.1"
                     /translation="MREIQFREALREAMVEEMRKDKSVFLIGEEVAEYNGAYKVSQGM
                     LDEFGPDRVIDTPISELGFAGLSVGAAMGGLRPIVEFMTFNFSLVAIDQIINSAAKML
                     YMSGGQVPVSIVFRGPTGNAGMLSAQHSQNFENWYANTPGLKVVVPSNPYDAKGLLKS
                     SIRDDDPVIFMESELMYGDKGAVPEGEFLIPIGQAKVVKEGKDVTLVSFGKIMKVAIE
                     ASTEMEKHGVSAEIIDLRTVRPIDYAAVVESVKKTNRLVIVEESWPLSSISTEITYHI
                     QKHAFDYLDAPVQRINNMDAPLPYASTLIEEILPNVKRTVEAIKSVMYI"
     gene            complement(3218..4984)
                     /locus_tag="VGA21_03695"
     CDS             complement(3218..4984)
                     /locus_tag="VGA21_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013453036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649636.1"
                     /translation="MRKLFYVVLIFATLSMSGCALNKMIKAAKDQDLTVVPNPLEVHA
                     DTVAFEISAVLPTKMLQKGKIYTVNSFYKYGDAEIAFEGIEFQADQFASSSTEQPRLS
                     KKFSFAYTDAMKSGEVQMMGVALDPKNGKTKESERLKVADGVITTSKLIQKTYFAAYA
                     PHGYNNAEEMEPINVNFYFDQGKSNLKRSEINSDRGKYFQGFIAEKNVTRTVTITGTH
                     SPEGAERINSRLSPDRAKTIEDYYKAQMKRYDYKGMADSINFILKPIIRDWADFMTAL
                     ASYEGITSEEKSAYQNIINGSGTFEEKENGMHKLPTYRKVFRDVYPGLRTAKTDILTV
                     KDKKSDAEISVLSKQVAQNETSADTLSVEELMYGATLTPSLTEKEAIYKAAIAKSDDW
                     NSHNNLGAVYVQMAIENPSEATSYADMAVTQLQISINKQESAEAYINLGSAQLMQGNI
                     RAALASLRTSGTKNPSGDNARGANGVLGALEAMTAQYDRAVIALSNSQETAVNLFNKG
                     LAQLLNKDFNNALASFQEAIQKDGNLALASYGAAVANARLQNEARVIENLTNAVTKDP
                     SLKELALNDLEFRNFNISSALN"
     gene            5132..5347
                     /locus_tag="VGA21_03700"
     CDS             5132..5347
                     /locus_tag="VGA21_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC family transcriptional regulator"
                     /protein_id="HEX9649637.1"
                     /translation="MRKIVDFFEDKAFGVCARLGEKFGIATSSIRLFFIYSSFITFGS
                     PVIIYLGLAFIMNIRKHLRRRWSMVWN"
     gene            complement(5344..6330)
                     /locus_tag="VGA21_03705"
     CDS             complement(5344..6330)
                     /locus_tag="VGA21_03705"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX9649638.1"
                     /translation="MPLVTIAILNYNGVSHLETFLPAVLKHSTGSELVIIDNGSTDAS
                     AKFIQENYPQVKLIQLNKNYGFCEGYNQGLKELSSDYYILLNSDVEVTPGWIDQIIEM
                     MESDNKIAACQPKILSYSERSKFEYAGAAGGFIDWLGYPFCRGRIFNHLEEDFGQYDE
                     PMQVFWASGACLFIKSHLFHEMGGFKSEFFAHMEEIDLCWRLQNAGYAIWCNPKSRVF
                     HLGGGTLDRFNPEKTYLNFRNNLATVFSNMTILQLFLVLPMRVVLDLVAAASFLFTGG
                     FRHFWAVFRAYFSLPGLVKKRGSTGSGQVRLYPSIVWATYIRGIRKFSDLKG"
     gene            complement(6330..6662)
                     /locus_tag="VGA21_03710"
     CDS             complement(6330..6662)
                     /locus_tag="VGA21_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009581560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEX9649639.1"
                     /translation="MFDMMKMMGKMKEVQVKIKEAQENLVHIKVTGESGGGMVRVTVN
                     GKKQLVKIEIEDDIFDKNDKELMQDLVVAATNKAIEEAEIRAKEELKKSTQGILPDIP
                     GFDLNNLM"
     gene            complement(6691..>7603)
                     /locus_tag="VGA21_03715"
     CDS             complement(6691..>7603)
                     /locus_tag="VGA21_03715"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type B sorting domain-containing protein"
                     /protein_id="HEX9649640.1"
                     /translation="EYLDSAIVCGQDYCYQILANFQGGGQAISVIACVTSFSTAQPDP
                     VTDVSVNVNNLVVEIEWPQPLNFTPDQYFLEASFDNSGFSLIRTLTTNFLSGLDSAGC
                     FRVYYQDLCGNTSARSPVGCQIMASFTETLNVLHISWNDYTGWESGVSNYRLEVFNQN
                     MDLISNYDAGLQNQFQLDLTLINEQVLIFKVIAVPVNGSLSDISSGLIVYIREPVISL
                     PRAFTPDQDGLNDTYQVYGLFIDTIAFLVFDRWGNIVFSEKELGSGWDGTIDGKPAPA
                     GAYVYNVEVTDFAGRTFKRSGSFLLLR"
BASE COUNT         2241 a         1591 c         1472 g         2299 t
ORIGIN      
        1 ttaaggagtt gaagaagaag ggagaaacgc ttacttttgc ttacttgttg ctctcatata
       61 ttgaagttta aaaatgataa attaaggtcg cgtggccgag tggctaggca gaggtctgca
      121 aaacctttta cagcggttcg aatccgctcg cgacctcaac cagaatccaa ccccttcgct
      181 atgcgaaagg gttttttaat tcccgggagg gaataaaatg tttgtaggca gtatgctgga
      241 attcaaatcg tcttttattt tgatttcctt ccggtaaata aaaaactatt gcaaatgaag
      301 ttggattttg gttgaagagt ccaccggaac cgggaacatt aacaattttc caagcccagt
      361 taaggctata atttgaaaaa aaattaaaat gaaataagga atttattttt tagaaaattt
      421 tttgagcaaa agtcatgaat aagaataatc caaagctcta tcatcataat gtttttgtta
      481 caattatatt cacaggattg ctggctggac ttcttgatgg tgccgcggct gttattcacg
      541 ttacaatact gggcagcacg caaccgatga aagtgtttaa ttacatcgcc agcgggatat
      601 ttggtgacgc cgcattttca ggagggatag caatgacata ctttggaatt ttctttcact
      661 tgggaattgc cacaatatgg accgccctgt ttttcctgtt ttataaagtt tttataaagg
      721 taacaggcaa ttggctcatc ctgggaattc tctacggaat aatagtatgg atgggaatga
      781 atctggtagt attaccctta agcaatacac ctcctcgtac attttcgatt aataatacga
      841 tgattggggt attaatacta ataacctgtg taggaattcc tgttgcttat tcttcattta
      901 aatttcaaaa taaaaggtga tcggaaattc agggaagacc taagaattta gcaaaagcta
      961 attatgttcc attatcttgc atctcatgaa aatatgcttc gccacatcag aaaaaacttt
     1021 gtcccttact ccttctgatc gaagtctttt tgaagctatg caaagaaatg atatggaagt
     1081 aagcgtatcc gcctggagcg ataggaaaat aaattggtct gaatttgatg tcattataat
     1141 ccgctcatgc tgggactacc atctacggat tgggcaattc aactcatggt tgaatgagct
     1201 ggatcggttg aaaatcaagg tgttcaatcc cattgaagtg ctaagaggaa atttagataa
     1261 gaaatacctt ggagatttac aggagaaggg agtccggata cccactacat tatggtttga
     1321 ggcagaagct aagcttgatt gcaaagacat attacggtct cagggttggc aggaggcggt
     1381 tctgaaacca aacatcggag ccactgctta cggcgtcaga aaaatttccc tgaaaacatc
     1441 agaaaattta ttcatcgaca ataatcctga attcttgaat ggtttcatgg ttcagaaatt
     1501 tattcccgaa attgaaactc agggcgagtg gtctataatt ttctttggaa aagaatacag
     1561 tcattcggta ataaaaaaaa caaatcgcgg tgagtttcga atccagtatg agtatggagg
     1621 atcctttaaa cccgctaatc ctgcccaggg agtaattagt gaagcacgaa gtatattgaa
     1681 gtattatccg gataatttat tgtatgcacg gattgatggt gttctggcag gtaataaatt
     1741 ttacctgatg gaagccgaac ttaccgaacc tgaattattt atttcggaaa gggtctttgc
     1801 ggaccggttc gtgtccagaa tgcgggaatt gttataaccg ctgagcgagg catattctcc
     1861 accgaaataa aatttgtttt agtggtaatg aaaaatggct gcacttctga cattacaaat
     1921 aagagttccc gggtatccgg gaagcaaaaa ggaaatcctc agtatccgct taaatgtaca
     1981 tcacggactt gattgcctca acggttctct taacgttagg aagaatttct tctatcagag
     2041 ttgaggcata gggcagggga gcatccatgt tgttaattct ttgaacaggc gcatccagat
     2101 aatcaaacgc gtgcttttga atatgatagg taatctcggt agagattgac gatagtggcc
     2161 aggattcttc aacaatgacc aaccgattgg ttttcttaac agactcaacc accgcagcat
     2221 aatcaatagg ccttaccgtc ctcaaatcaa taatctccgc cgagactcca tgtttttcca
     2281 tttctgttga tgcctcaatt gcaactttca taatcttccc aaatgaaacc agggtaacat
     2341 cttttccttc tttcaccact tttgcctgac caattggaat gagaaattca ccctccggaa
     2401 ctgcaccttt atcaccatac atcaattctg actccataaa aataaccgga tcatcatccc
     2461 ggatggagga tttcaataag ccctttgcat catatggatt tgatggaacc accactttta
     2521 atccaggagt gttcgcgtac caattttcaa aattctgcga gtgttgtgca cttaacatcc
     2581 ccgcattacc ggtgggaccc ctgaaaacaa tggaaaccgg cacctgacct cccgacatgt
     2641 acagcatttt agctgcacta ttgatgatct ggtcaatggc aacaagactg aagttgaatg
     2701 tcatgaattc aacaattggc cgtaagcctc ccattgcagc tccaacgctt aagcctgcga
     2761 agccaagctc tgaaatgggt gtatcaataa ccctgtcagg tccgaactca tccagcatgc
     2821 cctggcttac cttataggcg ccattgtact ctgctacttc ttcaccaatt agaaaaacac
     2881 tcttgtcttt cctcatttct tcgaccatag cctcgcgcaa agcttctcta aattgaattt
     2941 ctctcatctg gatttggaat tcgggttata caattttcat acggggagaa caaaaatact
     3001 gggtgtgagg caattaacaa acaaaagcaa aaaaaaggta aactctcagt ttgataaaaa
     3061 tgctacccaa ctgatatgca ctttgactga ggctttaggg aattttctaa gatcaatcta
     3121 aatatttatt ggtgtgttgc agggaaggtt taaaaatgag tcatcaaaaa aagccctggt
     3181 gggtttgcca gggcttttcg ataagtaatg agaaaaatta attcaacgca ctgctgatat
     3241 taaaattcct gaattcaagg tcatttagag ccagttcttt caaagaagga tccttggtta
     3301 ctgcattagt caggttttca ataaccctgg cttcattctg caatcgcgcg ttggcaactg
     3361 cagctccata acttgccaga gctaaattac catccttctg aattgcttcc tggaaggagg
     3421 ctaatgcatt attgaagtcc ttatttaaaa gctgagccag acctttatta aacaggttaa
     3481 ccgcagtctc ttgtgaattg gaaagggcaa ttaccgcccg gtcatattgc gcggtcattg
     3541 cttccaatgc tcccagtaca ccattagctc cacgtgcgtt gtcaccagat ggattcttgg
     3601 taccggaggt tcttaatgat gccaaagcag cgcgtatatt gccttgcatc aattgagctg
     3661 atcccaaatt aatataggcc tcagcacttt cttgcttgtt aattgaaatc tgcaactggg
     3721 ttacagccat atccgcataa ctagttgctt cagatggatt ttcaatggcc atctgcacat
     3781 atactgcacc caaattgttg tgagaattcc aatcgtcgct ttttgcgata gcagctttat
     3841 aaatggcttc tttttccgtc aggcttggag taagagttgc cccatacatt aattcttcaa
     3901 cagataaagt atccgctgaa gtttcattct gagctacctg cttggaaagg acagaaattt
     3961 ctgcatcgga ttttttgtct tttacggtaa ggatatcagt tttcgcggtt cgcaaacccg
     4021 gataaacatc cctgaatact tttctataag taggtagttt atgcatccca ttttcctttt
     4081 cttcaaatgt tccgcttcca ttaataatat tttgataagc agatttctcc tcgcttgtta
     4141 ttccttcata tgaagccaat gcagtcatga agtctgccca atctctgatt ataggtttaa
     4201 ggatgaaatt gatactgtct gccattcctt tataatcata tctcttcatc tgagctttgt
     4261 agtaatcctc aatcgtcttg gctctatcag gtgatagccg ggagttaatt ctttcagcac
     4321 cttcaggtga gtgagtacct gtaatagtta ctgttctggt aacatttttc tcagctataa
     4381 agccttggaa atacttgcct ctgtcactat tgatttcgga ccgtttcagg ttagatttcc
     4441 cctgatcaaa ataaaaatta acgttaatag gctccatttc ctcagcatta ttgtacccat
     4501 ggggagcata agcggcaaaa taagttttct ggatcagctt ggaagtagtg ataaccccat
     4561 cagctacttt caggcgctca gattctttag ttttgccgtt tttgggatca agtgccacac
     4621 ccatcatctg cacttcacca cttttcatgg catcagtgta tgcaaagcta aatttctttg
     4681 atagccttgg ttgttcagtt gagctgctgg caaattgatc ggcctgaaat tcgatgcctt
     4741 caaaagcgat ctctgcatcg ccatatttat agaaggaatt cactgtataa attttaccct
     4801 tttgtaacat cttggtgggc aatacagcag aaatttcaaa ggcaaccgta tctgcatgga
     4861 cttcaagcgg atttgggaca acggtaaggt cctgatcttt ggcggcttta atcattttat
     4921 tcagagcgca acccgacatg cttaaggttg caaaaatgag taccacatag aataattttc
     4981 tcatcggatt atatagtttg attttatctt tggttgaatt aattgcgaaa ttatcgtgaa
     5041 tctggcatta atgcaattta aggccataaa aataatgttt tattttagcc gaacagacaa
     5101 tataagtata tttgcttaat tggttctaaa tatgagaaaa atagttgatt tttttgagga
     5161 taaagcattc ggagtttgtg ccaggctggg tgaaaaattt ggaatagcaa cttcctctat
     5221 ccgcctcttt ttcatttaca gctcattcat aacttttggc tcccctgtaa ttatttacct
     5281 tggtttagct tttataatga atatcagaaa acaccttaga agaaggtgga gtatggtctg
     5341 gaattagccc tttagatctg aaaatttcct aatacctctt atgtaggttg cccacacgat
     5401 gctgggatag agcctgacct gaccactccc tgtggatcca cgttttttca ctagtccggg
     5461 tagtgagaaa taagctctga atacagccca aaaatgccta aatcctcctg taaaaagaaa
     5521 acttgccgcg gcaactaaat ccaatacaac tctcattggg agtaccagga aaagttgcag
     5581 tatggtcata ttgctaaaaa ctgtcgccag attgtttctg aaattcaaat aagtcttttc
     5641 ggggttgaac ctgtccaatg tgcctccgcc taaatgaaaa accctgcttt tgggattgca
     5701 ccaaatagca taacccgcat tttgtaagcg ccagcataaa tctatttctt ccatatgggc
     5761 aaaaaactcc gatttaaacc cacccatttc atggaataaa tgcgatttga taaaaagaca
     5821 ggccccggaa gcccaaaaaa cctgcatcgg ctcatcatat tggccaaaat cttcttctaa
     5881 atgattaaaa atccttcccc tgcaaaaagg ataccccagc cagtcaataa atccgcctgc
     5941 tgcacccgca tattcaaatt tcgaacgctc tgaataggaa agaatcttag gttggcacgc
     6001 agcaatttta ttgtcacttt ccatcatttc tatgatctga tcaatccatc ccggggtcac
     6061 ttccacatca gaattcaaaa gtatatagta atctgatgac agttctttaa gaccctggtt
     6121 atatccctca caaaacccat aattcttgtt gagttggata agtttcacct gcgggtaatt
     6181 ttcctgaata aactttgcag aagcatcggt tgatccatta tcgataatca ccaattcaga
     6241 tcccgtgcta tgctttaaaa cagcaggaag aaaagtttcc agatgagaga caccattata
     6301 attgagaata gcaatggtga ctaatggcat tacatgagat tattgagatc aaagccgggg
     6361 atatccggaa gaattccctg agtgcttttc ttgagctctt ctttagcccg gatttctgct
     6421 tcttcaatgg ctttatttgt tgcagctacc accaggtcct gcatcagctc tttatcattt
     6481 ttgtcgaaga tatcatcttc gatttcaatt ttaaccagtt gcttctttcc attaacggtt
     6541 acccgaacca taccaccacc actttctccg gtaactttta tatgaacaag attttcctgc
     6601 gcttctttta tctttacctg aacctccttc atctttccca tcattttcat catatcaaac
     6661 ataatcttcc ttttttgtaa atttagatat ttagcgtaaa aggaggaaac ttccggaacg
     6721 tttgaaagtt cttccggcaa aatctgtcac ttcaacgtta tagacataag cgcctgcagg
     6781 agcaggtttg ccatcaattg tgccatccca accactgcct aattcttttt ctgaaaacac
     6841 aatattgccc cagcgatcaa acaccagaaa tgcaatggtg tcaataaata agccatatac
     6901 ctggtaggta tcgtttaaac catcctgatc cggggtgaag gcacgaggta agctgataac
     6961 aggttcccgg atgtatacta tcaggccgga agagatgtca ctcaagcttc cattgaccgg
     7021 aacggctatc actttgaaga tcaatacttg ttcattaatt aatgttaaat cgagttgaaa
     7081 ttgattttgc agtcccgcat cgtaattgga aattaaatcc atattctgat tgaaaacctc
     7141 aagcctgtaa ttgcttactc cggattccca gcccgtataa tcattccagg aaatgtgtaa
     7201 gacattcagg gtttcagtaa atgaagccat gatttggcag ccaaccggac ttctggccga
     7261 agtattaccg cacaaatcct gataatacac ccggaaacat cctgcggaat ccaatcctga
     7321 caaaaaatta gttgtaagtg tgcggatcag gctgaatccg gaattatcaa aacttgcttc
     7381 aagaaaatat tggtccggtg taaaattcaa aggctgaggc cattcgattt caacaaccag
     7441 gttattaaca ttaaccgaaa catcagttac cggatcgggt tgggcagttg aaaaagacgt
     7501 tacacatgct ataacagaaa ttgcttgtcc gccaccctgg aaattcgcca aaatctgata
     7561 acagtaatcc tggccacaaa cgattgcaga atccagatac tcc
//
LOCUS       DASWHX010000053         6089 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_1891328, whole genome shotgun sequence.
ACCESSION   DASWHX010000053 DASWHX010000000
VERSION     DASWHX010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 6089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6089
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1891328"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..939)
                     /locus_tag="VGA21_03720"
     CDS             complement(<1..939)
                     /locus_tag="VGA21_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008202438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HEX9649641.1"
                     /translation="MTGKKEMMKSLGEVNASVDAMNKKGFWRKLFAFMGPAYLVSVGY
                     MDPGNWATDIAGGSQFGYKLIWVLLMSNIMAILLQSLSARLGLIRKMDLAQASRATYN
                     KFVNFCLYILAEIAIAAMDLAEVLGMAIGLQLLTGLPLLWGVSITVADTLLLLLLYQY
                     GMRKMEAFIVSLVAIIGGAFFVEMILAKPDMSELVTGFIPSLPNDTALYIAIGIIGAT
                     VMPHNLYLHSSLVQTRRIDNSDKGLWDAIKFNIIDSAIALNMAFFVNAAILVLAAATF
                     YEIGLYQVEEIQDAHKLLAPLLGTEWAAILFAVALIA"
     gene            complement(941..1603)
                     /locus_tag="VGA21_03725"
     CDS             complement(941..1603)
                     /locus_tag="VGA21_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009186417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEX9649642.1"
                     /translation="MMKLSYSEENYLKAIYHLSENRNQGVNTNAIADSLNTKAASASD
                     MIKKLAAKKLISYKKYYGVKVTDTGKKVALQIIRKHRLWEVFLVQKLNFNWDEVHEVA
                     EELEHIKSPLLIQRLNEFLGDPKFDPHGDPIPDETGTIPSKPSIPLSQLITGKSGIIT
                     SVKDSDSSFLKYLDKMEMKIGSRIKVLDVIEFDNSMEILIDDKRKINVSNEVSGNLLM
                     KI"
     gene            complement(1675..2784)
                     /locus_tag="VGA21_03730"
     CDS             complement(1675..2784)
                     /locus_tag="VGA21_03730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649643.1"
                     /translation="MQKSIVIASILKPVSDTRMYEKFAQTLSMNSKYKVIIVGFASKK
                     IPIGEGITLVPLFQFSRLSIQRLLAQFRFFFFLTNTKPDLVIINTHELLGIAILAKYI
                     YGIKVIYDVRENYFRNVAYGRSLPILIRWPLALAIRLTESFYSLFADFFFIAEAGYAK
                     ELPFIKSKFVVLENKVSKLVINKIPITRNPSDNKFLVCGTISEEYGIFEAIDFVSKLR
                     TRIPEASLKIVGYCANGRLWKKIAKRIAGLEYVAVIGGDELVPHEIVLHSMAETNYIL
                     MPYENLPQIRHCFPTKIFEAVALNCPILISYGETWNSFITNYSAGLQVDFHQVIPIEV
                     IHEARSHQFFTKSIPDTIYWESQENLLQEAVIKLF"
     gene            2820..3632
                     /locus_tag="VGA21_03735"
     CDS             2820..3632
                     /locus_tag="VGA21_03735"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018474017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HEX9649644.1"
                     /translation="MQMQEIIERAWEDRSLLKEKTTVETINSVIEQLDKGLVRVAEPS
                     DGGWKLNEWVKKAVILYFPIQEMKVIEVGPFEFHDKIKLKSNFKDLGVRVVPHGLARY
                     GSYLARGVIMMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSGGVGIGGVLEPV
                     QAAPVIIEDNVFVGSRAIIVEGIRVGKEAVIGANVTLTGSSKILDVTGRETKEYKGYV
                     PERSVVIPGSYTKEFPSGNYNVNCALIIGKRKESTDKKTSLNQALRENDIAV"
     gene            3697..4290
                     /locus_tag="VGA21_03740"
     CDS             3697..4290
                     /locus_tag="VGA21_03740"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9649645.1"
                     /translation="MKRTIQIILLCLLITACRDEGSVQGDQLFEQGKYEEAIAKYDEY
                     LRMKPSHLKTIYNRGRAYEELGIYEKARVDFTRVLEIDPDNVSAYLSLGNYFYRFEEY
                     SNAYFYYDLAIERDGNSAAGYLLRGKTSHKMGQFRDAISDYNAAIRIDRELGEAYLSR
                     GAIEIIQNQNKRACSDFQMANSLGVRDAAEAMRKYCQ"
     gene            complement(4292..5179)
                     /locus_tag="VGA21_03745"
     CDS             complement(4292..5179)
                     /locus_tag="VGA21_03745"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010610645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HEX9649646.1"
                     /translation="MKNISVIGAGTMGNGIAHVFAQNGYAVTLIDVQTTALEKAIQTI
                     RKNLDRQVNKEIISEDDKKKILARIVTESDLAKAGGSDLIIEAATENVDLKLKIFRQL
                     DETAGKDTVLASNTSSISITKIASVTQRPDKVIGMHFMNPVPVMKLVEVIRGYNTSNQ
                     VTKNIMELSEKLGKIPVEVNDYPGFVANRILMPMINEAIYSLHEGVAGVSEIDTVMKL
                     GMAHPMGPLQLADFIGLDVCLSILRVLYDGFGNPKYAPCPLLVNMVHAGHLGVKSGSG
                     FYEYTPGSKDLAVASMFRK"
     gene            complement(5238..5768)
                     /locus_tag="VGA21_03750"
     CDS             complement(5238..5768)
                     /locus_tag="VGA21_03750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649647.1"
                     /translation="MKTCLPTVLLLLPLIVFAQNDLDEKKGFQDITLGSDPKVYQGIN
                     LKKTTKDENFKKNSKDENIPEVDLYQAKGGYYQDVAGIKIHKFIVKAFDNRIYEIEIT
                     AEKDPDFYKGLESRYGPGNYSMPTNSYIWSGKEVRLSFKSESKKKVKLTYFLFAVNEW
                     IKEKEKENAKSIADDF"
     gene            complement(5837..>6089)
                     /locus_tag="VGA21_03755"
     CDS             complement(5837..>6089)
                     /locus_tag="VGA21_03755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649648.1"
                     /translation="KSLRKVEISSLMEQRTKLMGLSEEEIIKLLGNPDETNLYQRTQK
                     FLVYYTSPGPKCDMPEAGASRFEIRISALHVVNEVVHYP"
BASE COUNT         1811 a         1204 c         1239 g         1835 t
ORIGIN      
        1 tgcaataagc gcgaccgcaa atagtatcgc tgcccattct gttccaagta gcggtgcgag
       61 taatttgtgc gcatcctgta tttcttccac ctggtacaaa ccaatttcat aaaaagtggc
      121 agccgcaagc acaagtattg ctgcatttac aaaaaatgcc atgttcaggg caattgcaga
      181 atctataatg ttgaacttga ttgcatccca caatcctttg tcagaattgt caatccggcg
      241 ggtttgaaca agcgatgaat gcaggtagag attatgcggc atcactgtag ctccaataat
      301 tcctatggcg atatatagcg cggtatcgtt gggaagagaa ggtataaacc ccgtgaccag
      361 ctcactcata tctggttttg caagtatcat ctcaacaaaa aatgcacccc caatgatggc
      421 tacaaggctt acgatgaagg cttccatttt tcgcattcca tattgatata aaagcaaaag
      481 cagtagagtg tccgcaacag tgatgcttac tccccataat agtggaagtc cggtgagtaa
      541 ctgtaaacca attgccattc ccagaacttc agcaaggtcc attgctgcaa tggcaatttc
      601 tgcaagaatg tataaacaga aattgacaaa tttattatag gtagcgcggg aggcttgggc
      661 aagatccatt ttgcgaatta accctaacct ggcacttaag ctttgcaaaa gaatcgccat
      721 gatgttgctc atcagcaaca cccaaatcaa tttataaccg aactgacttc cgccggctat
      781 atcggtcgcc cagtttcccg ggtccatgta tccaacagag accaggtaag ccggccccat
      841 gaaagcgaat agctttctcc agaaaccctt tttgttcatc gcatcaacgg aagcattaac
      901 ttcacccagc gatttcatca tttccttctt acccgtcatt tcaaatcttc attaacaggt
      961 tacctgaaac ttcattggag acattgattt tccttttgtc atcaattaga atctccatac
     1021 tgttgtcaaa ttcaattaca tctaaaacct ttattcttga accaattttc atttccattt
     1081 tatccagata tttcagaaag ctgctgtcac tatcctttac cgaagtaatt attcctgact
     1141 tccctgtaat gagctgactt aggggaatgg aaggtttaga aggaatggtt ccggtttcat
     1201 cagggatggg atctccatga ggatcgaatt ttggatctcc tagaaactca ttcaatctct
     1261 gtattagcag gggagacttg atgtgttcta attcctcggc aacttcgtgc acttcatccc
     1321 aattaaaatt cagtttttga accaggaaaa cttcccataa cctgtgtttc cttataatct
     1381 gaagtgcaac tttcttaccg gtatccgtaa ccttaacccc gtagtacttc ttgtaactga
     1441 tcaatttttt tgccgcaagc ttttttatca tgtcgctcgc tgacgccgct ttggtattca
     1501 gcgaatctgc tatagcattg gtgtttactc cctggtttct gttttccgaa aggtgatata
     1561 tagctttaag atagttctct tccgaataac tcaatttcat aatcgtaatg ttggtgcaaa
     1621 tgtaaaatta atttagacaa aactaaaata atttttttga atagtaaaaa aaaatcagaa
     1681 aagtttaatt accgcttcct ggagtagatt ttcctgggat tcccaatata tggtatcagg
     1741 aattgatttt gtaaaaaact gatgcgacct tgcttcgtgt atcacttcaa tagggataac
     1801 ctgatgaaaa tccacctgta agccggctga ataattggta atgaaactat tccaggtttc
     1861 tccatacgaa ataaggatag ggcagttaag ggcaactgcc tcaaaaatct tggtcggaaa
     1921 gcaatgcctg atttgaggta aattctcata gggcataagg atataatttg tttccgccat
     1981 ggaatggaga acaatttcat ggggaaccaa ttcatccccg ccgataaccg ctacatattc
     2041 aagcccagca attcttttgg caattttttt ccataacctt ccattggcac agtatccgac
     2101 tatcttcagc gaagcttcag gaattctggt tcttaattta gaaacaaaat caatggcttc
     2161 gaaaattcca tattcttccg aaatagttcc gcagacaaga aacttgttat cagatggatt
     2221 tctggttatg ggaattttat tgattaccaa tttggatact ttattttcta atacaacaaa
     2281 tttgcttttt atgaatggta actctttggc atagccagcc tcggcgataa aaaagaagtc
     2341 tgcaaaaaga gaataaaatg attcagtcag gcgaatagcc aaagccaatg gccaccggat
     2401 taatattgga agtgaccttc cgtaagctac attgcgaaag tagttttcgc gcacatcgta
     2461 aatcacttta attccataga tatattttgc cagtatggcg atgccaagaa gttcatgcgt
     2521 atttatgata accagatcag gttttgtatt ggtgagaaaa aaaaagaatc tgaattgtgc
     2581 taaaagcctt tggatgctga gcctcgaaaa ctggaataaa ggcaccaggg taattccttc
     2641 accaatgggg attttttttg aagcaaaccc aacaataatt accttatatt tggaattcat
     2701 ggaaagagtt tgtgcaaatt tctcatacat cctggtgtcg gaaacaggtt taagaatgga
     2761 tgctatgaca attgattttt gcacaatgcg acaagttagc ttttaattag aatttgtaaa
     2821 tgcagatgca ggagataatt gaaagagcct gggaagacag atcgcttctg aaagaaaaga
     2881 cgaccgttga aacaatcaat tcggtaatag aacaattgga taaaggactg gttcgcgttg
     2941 ctgagccttc tgatggtggc tggaagttaa atgaatgggt gaagaaagcc gtgattttgt
     3001 actttcccat tcaggagatg aaagtaatag aggttggacc ctttgaattt cacgacaaga
     3061 taaaattgaa atcgaatttt aaagatcttg gagtccgtgt agtacctcat ggtcttgcaa
     3121 gatacggatc ttacctggcg aggggagtta tcatgatgcc ttcatacgtg aatatcggag
     3181 cttatgtgga tgagggtacg atggtcgaca cctgggcaac ggttggaagt tgtgcgcaaa
     3241 ttggcaagaa tgttcacctt agcggtggag ttgggattgg tggggtactg gaaccggttc
     3301 aggctgcgcc ggtaataatt gaagacaacg tgtttgtagg atcacgtgct attattgttg
     3361 aaggaattcg cgtgggaaag gaagccgtga ttggtgccaa cgttaccctt accggaagtt
     3421 ccaaaatact ggatgtgacc ggcagggaaa caaaggaata taaagggtat gttcccgaaa
     3481 gatcggtagt tataccggga tcgtatacaa aggaattccc gtctgggaac tacaatgtaa
     3541 attgcgcatt aataattggc aagagaaagg aaagcacaga caagaagact tcattaaatc
     3601 aggcattaag agagaacgat atcgctgtat aatttttttg cactattttt gccagcttaa
     3661 aagcgacaag gaaaagaaat tggtgtcata agattcatga agagaacaat tcaaataatt
     3721 ttgctttgct tattaataac cgcttgcagg gatgaaggtt cagtgcaggg agatcaatta
     3781 tttgaacaag ggaaatacga agaagcaatc gcaaagtatg atgaatattt gagaatgaag
     3841 ccttctcatt tgaaaacaat ttacaatcgc ggccgcgcgt atgaagagtt aggtatttac
     3901 gaaaaagcta gagtagactt tacccgagtt ttagaaattg atcctgataa tgtaagcgcg
     3961 tatctttcct taggtaatta tttttataga tttgaagagt attcaaacgc gtatttttac
     4021 tatgatctgg ccatagagcg tgatggaaac agtgccgcgg gatatctcct tcgcggcaaa
     4081 acgtcccata agatggggca attcagggat gccatttcgg attacaatgc cgcaattcga
     4141 attgataggg aattgggaga agcctactta tcccgtggag ccattgaaat cattcagaat
     4201 cagaataaaa gagcttgctc tgactttcag atggcaaatt ccctgggtgt cagggatgca
     4261 gccgaggcaa tgagaaaata ttgtcagtag tttattttcg aaacatggaa gccaccgcca
     4321 gatcctttga tccgggcgtg tactcgtaaa accctgaacc ggatttcact ccaagatggc
     4381 ccgcgtgtac catatttacc aacaaaggac aaggagcgta ttttggatta ccaaagccat
     4441 cataaagcac ccgcaaaata cttaagcaaa catccaggcc aataaaatct gcgagttgca
     4501 gcggccccat aggatgagcc atgccaagtt tcattaccgt gtcaatttca gatactccgg
     4561 caactccttc atgcaaggaa taaatagctt catttatcat tggcatcagt atccggtttg
     4621 cgacaaaacc tggataatca tttacttcaa ccggaatttt ccccagtttc tcagataact
     4681 ccatgatgtt cttcgttacc tggttggacg tattatatcc cctgattact tccaccaatt
     4741 tcattacagg tacaggattc atgaaatgca ttccaataac tttatcaggt ctttgcgtta
     4801 ccgacgcgat tttcgtaatt gaaatagaag atgtatttga agcaaggaca gtgtctttac
     4861 cggcagtttc atccaactgc ctgaatattt tcaatttcaa atcaacgttt tccgtagctg
     4921 cttcaataat caaatctgaa ccgccggctt tggcaagatc tgattcggtt acgattctgg
     4981 ccaggatttt cttcttatca tcttcagaaa tgatttcctt attaacttgc ctgtcgaggt
     5041 ttttacgaat ggtctgaata gctttttcga gcgccgtagt ctgcacatca ataagagtta
     5101 cggcataacc gttttgagca aaaacatgcg ctatgccatt acccattgtt cccgctccaa
     5161 taactgagat gtttttcatc gcgataaatt tgcctcgcta agatatgaag atttgagttg
     5221 ccgggaaaat gactttctta aaagtcgtcg gcaattgatt ttgcgttttc tttttccttt
     5281 tctttgatcc actcatttac tgcaaaaaga aaataggtga gtttcacttt tttctttgat
     5341 tctgatttaa aagagagtcg cacttctttt ccgctccaaa tataggaatt ggttggcatt
     5401 gaataatttc ctggtccata tctggactca agtcctttgt aaaagtcagg gtctttttca
     5461 gcagtaatct caatctcata aatcctatta tcaaaagcct taacaataaa tttatggatt
     5521 ttgatccctg ctacatcctg gtagtatccg ccttttgcct ggtataaatc aacttcagga
     5581 atgttttcat cctttgagtt tttcttaaaa ttctcatctt ttgtggtttt cttcaaatta
     5641 attccctggt aaacttttgg atcgctgccc agggtgatat cctgaaaacc tttcttctca
     5701 tcaagatcat tctgagcaaa gacgattagc ggaagtagta ataatactgt aggcagacaa
     5761 gttttcattt cgattcaagt aatctttatc caaaggtaag aaaattaaca gggaatgaaa
     5821 tgtaaatacc ggataatcat ggatagtgaa caacctcatt aactacgtgg agcgcgctaa
     5881 tcctgatttc aaaccgtgaa gcaccagctt caggcatatc gcatttaggt ccgggagacg
     5941 tgtagtagac cagaaatttc tgcgtccttt ggtacaagtt tgtttcgtca ggatttccca
     6001 atagtttgat tatttcttct tccgaaagtc ccattagttt tgtccgctgt tccattagtg
     6061 atgaaatctc taccttcctt aatgacttg
//
LOCUS       DASWHX010000054        61421 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_36458, whole genome shotgun sequence.
ACCESSION   DASWHX010000054 DASWHX010000000
VERSION     DASWHX010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 61421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61421
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36458"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..810)
                     /locus_tag="VGA21_03760"
     CDS             complement(<1..810)
                     /locus_tag="VGA21_03760"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009581369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX9649649.1"
                     /translation="MSACGEKDYLVVIHTEYGDMKAILYEETPQHKENFVKLVKEGFY
                     DSLIFHRVIQGFMIQGGDPDSKNAGRGQRLGSGGPGYTIPAEIRTGFFHEKGALSAAR
                     LSDQINPGKESSGSQFYIVQGQTYAPDQLNADNVKYGKMVGLLNRMFYEGKNPELLQE
                     FIALQQAGDMEALKRKTFESLSIIEQEYGKQTFPEISSRQIEAYTKNGGALHLDGEYS
                     VFGKVVEGLDVIDKIAAVPTDQSERPTTDIIMIITLEEITKKEIARKYGYSY"
     gene            complement(861..1997)
                     /locus_tag="VGA21_03765"
     CDS             complement(861..1997)
                     /locus_tag="VGA21_03765"
                     /inference="COORDINATES: protein motif:HMM:NF024575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEX9649650.1"
                     /translation="MRFEEIPGLPEVKKSLLKSVKEEKVAHAQIFAGIEGGANLALAR
                     AYISYLYCEKRTDSDSCGECPSCQKNRKLVHPDVHFVYPILNTKDGKKIDPISKAYIK
                     EWRSFLPSNPYPVYEDWVSFFGGENKKAIIYREESREIIKDLTLVSFEGNFKTMLIWL
                     PELMHISAANGILKILEEPPPKTLFILVSNQASQVISTILSRCISINVRPFFDKEIAQ
                     FLVDIGMGAEKAGQISHLADGSLNMAFKLEENLEDDNHTRLIIWMRACFEFNVMEMVN
                     GSDTFSKRSKISQKSLFEYGLNMLRESLVIGFSSSIQLAIESEKAFIRDFSKIIDWEL
                     IEEMSKELESAIVYLERNANARIVYLDLSLRFASLFKQIKTVKS"
     gene            complement(1998..3014)
                     /locus_tag="VGA21_03770"
     CDS             complement(1998..3014)
                     /locus_tag="VGA21_03770"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9649651.1"
                     /translation="MKPHSGNIYLILAFTFLIGNKFDSVKAQDKGAEFLAIARDNLRE
                     GDRFSALITLDSALLKSPKYADAYLLRATVRTEIQNLEGAIEDLSNVIRIDPGRAEAY
                     YRRAMVYSDLGYHRDYCLRDLSEAITLDRKQSRYYVERARIRASSINTYNGNREYDLA
                     IDDINRAIAVEPRNGRLYYLRAGYKLKLEERDQALQDYSSAIELVPDNARFYSDRGLL
                     YLVLENYEKSALDYRKAISLDPENPEYYRNLAHAFYNYGNFRSSINAFSESINLLIKK
                     IRDNSSKEEMAKITDELKEVYLMRGSAFANMGREGEACDDFGRSRELGERRALNYMRK
                     YCGK"
     gene            3118..4419
                     /gene="rmuC"
                     /locus_tag="VGA21_03775"
     CDS             3118..4419
                     /gene="rmuC"
                     /locus_tag="VGA21_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009579182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEX9649652.1"
                     /translation="MEIVWFILGIVLGGVVSWFLATYRFKSNLSSNETAVKVYDERIK
                     DMIALLENARDEVKRERDKVITLSNRLSGNEADYRNLKERFEQQKEEVAKLQERFSIE
                     FKNLAQDILEEKSRKFTIQNKENLEGILKPLSERIIEFEKTVQQNSKESIEWNSALRE
                     QIKSLKELNIQVTKEAENLTKALKGDNRAMGAWGEIILESILENSGLVKDREYIVQSV
                     FVHDDGRRYQPDVVINLPDDKHLVIDSKISLVSYERFVNAETKEDTKIAIKSHLTSIR
                     SHIRDLSSKNYQTLYGVKSLDFVLMFIPIESAFSLAVQNDSYLFNEAYDRNIVLVSPT
                     TLIATLRTIANIWKNEYQNRNALEIARQGGELYDKFVNFTEDLLTLGKHLDQTQKHYS
                     SAMKKLVEGQGNLISKATKIKELGAKTRKSIDQRLIERSED"
     gene            4442..4816
                     /locus_tag="VGA21_03780"
     CDS             4442..4816
                     /locus_tag="VGA21_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020528529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES family protein"
                     /protein_id="HEX9649653.1"
                     /translation="MSNQAKLQKLIIVGDRVLIKPKKMSERTSSGLYLPPGVQEKEKI
                     QSGYIMKVGPGYPIPVPAEEDEAWKEKDEQIRYIPLQTKEGDLAIFLQRGAIEIMYEG
                     VKYLIVPQSSILMLERDEELFS"
     gene            complement(4895..6133)
                     /gene="rocD"
                     /locus_tag="VGA21_03785"
     CDS             complement(4895..6133)
                     /gene="rocD"
                     /locus_tag="VGA21_03785"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015264421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004587 - ornithine aminotransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HEX9649654.1"
                     /translation="MLELEISAQQAIELEDKYGCHNYHPLPVVLSKGEGVFLWDVEGK
                     KYYDFLSAYSAVNQGHCHPRLVKALINQARELTLTSRAFYNDKLGLTEKFVCEYFGYD
                     KALFMNSGAEANESAIKLARKWGYMVKGIPANKAVIVAVGRNFHGRTTTIISASTDPV
                     ARANFGPFMPGFEIIPYDDISALEGALQNSNVCGFWVEPIQGEAGVYVPSDGYMAKVA
                     ELCHRYDVLLMADEIQTGIGRTGKLLACDHENVHPDILILGKAISGGMMPVSLVLASD
                     AIMNTIKPGEHGSTFGGNPLACAVTMEALQIVKDENLSQNAQKMGEIFRSRMEMLVDK
                     FDIIKLVRGKGLLNAVVINDSEAGDTAWNICMKLKEKGLLAKPTHGNIIRFAPPLIIA
                     KDQILDCSAIIEDTFDEMFG"
     gene            6255..6497
                     /gene="rpmB"
                     /locus_tag="VGA21_03790"
     CDS             6255..6497
                     /gene="rpmB"
                     /locus_tag="VGA21_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009053262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEX9649655.1"
                     /translation="MSRVCQVSGKRPMVGNKVSHANNKNKRRFYPNLHTKRFYIPEED
                     RWVTLKVSASTMRTINKNGISKVLREAKKNGLYTGK"
     gene            6524..6706
                     /gene="rpmG"
                     /locus_tag="VGA21_03795"
     CDS             6524..6706
                     /gene="rpmG"
                     /locus_tag="VGA21_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011584052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEX9649656.1"
                     /translation="MAKKGNRVQVILECTEHKNSGQPGTSRYITTKNRKNTPDRMELK
                     KYNPILKKYTLHKEIK"
     gene            6710..6865
                     /locus_tag="VGA21_03800"
     CDS             6710..6865
                     /locus_tag="VGA21_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009579192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4295 domain-containing protein"
                     /protein_id="HEX9649657.1"
                     /translation="MAKKVVATLKNKEAKNFAKVIRMVRSEKTGAYTFKEEIVPQEDV
                     QKVLAKK"
     gene            6972..7934
                     /gene="ftsY"
                     /locus_tag="VGA21_03805"
     CDS             6972..7934
                     /gene="ftsY"
                     /locus_tag="VGA21_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018471880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEX9649658.1"
                     /translation="MAAFKGLFSPDKKETLDKGLEKTKENFLTRISKAVLGKSAVDEE
                     VLDNLEEILISSDVGVQTTIKIIQKIEERVARDKYLNASELDKILREEIGKLLEENNT
                     GDLTDFEIPKTAKPYVILVVGVNGVGKTTTIGKLAAQFKRIGKSVVLGAADTFRAAAV
                     DQLKQWGNRVGVPVVAHGQNTDPASVAYDTVKTAMDQGADVVIIDTAGRLHTKINLMS
                     ELSKIKRVVQKFRPDAPNEVLLVLDGSTGQNAVIQAREFTKATDVTAIAITKLDGTSK
                     GGVVIGISDEFKIPVKYIGVGEKPEDLQVFNKAEFVDSLFSERN"
     gene            complement(7931..8632)
                     /locus_tag="VGA21_03810"
     CDS             complement(7931..8632)
                     /locus_tag="VGA21_03810"
                     /inference="COORDINATES: protein motif:HMM:NF039966.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6048 family protein"
                     /protein_id="HEX9649659.1"
                     /translation="MIIRILFLILIGVPVTVFAQSKWVPSAVRVGVDLYDIVLTTVKS
                     NRTIYEFNADVDFHKYFFSFDYGIFEGSESNVIYDYSSKGYFWRLGLDINLTPYEKSR
                     SVVFFGLRYAEAAFDDKVQFDFNSYYFGNGRVIRENKDLYATWYEMVMGLKVPIWKFV
                     QLGLTGRMKFGRNMKTSNSLESLLVPGYGRGISRTEFAINYHLFFRIPFRNKPIPPKP
                     ERKKAAVEAATQSVN"
     gene            complement(8622..9083)
                     /locus_tag="VGA21_03815"
     CDS             complement(8622..9083)
                     /locus_tag="VGA21_03815"
                     /inference="COORDINATES: protein motif:HMM:NF040109.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6452 family protein"
                     /protein_id="HEX9649660.1"
                     /translation="MRKESLIIRLAFTLALLLIVFVSCRRAQNCTPVSRDFVIIQFVD
                     SLGVPKNEILTITSAASDSILVTANQITTQFNLPLDPFRDNVTYLVTQSGVENWITLG
                     YRNAVFINSDECGPEFVFSDLAIWGTSYDSVRLVNTRILEGIDVNVRIYDY"
     gene            9190..10503
                     /gene="rimO"
                     /locus_tag="VGA21_03820"
     CDS             9190..10503
                     /gene="rimO"
                     /locus_tag="VGA21_03820"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017731175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HEX9649661.1"
                     /translation="MKTKARKNKVNIVTLGCSKNLVDSEVLLTQLRGNKIDVTHENQE
                     DDANIIIINTCGFIDNAKQESVDTIIRYADAKKEGLIDKVFVTGCLSQRYKEDLEREI
                     PEVDAFFGTRELPQLLRRFKASYKYELLGERTTTNTHYAHMKISEGCDRPCSFCAIPL
                     MRGNHISRPIEELVTEARNLARNGVKELLLIAQDSTFYGIDLYGKRKLAELMSRLSDI
                     DGLEWIRLHYAFPTGFPKDVLKVMASKENICNYLDIPLQHGSSKMLQLMRRGITREKT
                     ELLLQEIRATVPEIAIRTTLIAGHPGETEFDFEEMKGFVEKSRFERLGVFTYSHEEGT
                     HSFSMQDDVPAAVKQVRADAIMDLQEKISFELNQAKVGKEFKVLIDRIEGANYVGRTE
                     YDSPEVDNEVIISSENNYLRIGDFSMIKITRAEEFDLFGIPVQPA"
     gene            10519..12081
                     /gene="bshC"
                     /locus_tag="VGA21_03825"
     CDS             10519..12081
                     /gene="bshC"
                     /locus_tag="VGA21_03825"
                     /inference="COORDINATES: protein motif:HMM:NF021586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis cysteine-adding enzyme
                     BshC"
                     /protein_id="HEX9649662.1"
                     /translation="MRVEKIELVKTNRFSQILINYLKQDDVFLEQFYDLYPSINNFEK
                     QIRAKIFAKSIRDELFQSLTEQYEGYQLSDKAQINLQSLKKENTFTITTGHQLSIFTG
                     PLFFHYKIITVINLCRKLSQKYPNYNFVPVFWMATEDHDFDEIAKVNIGGIDITWDTV
                     QAGPVGKFDPTGLKAVLGKIPADVGIFQESYLNESTLSNAVRNYVDEIYGKEGLVVID
                     GDDKRLKTAISDLIIEDCCSNNVNRLSEASSSELEKLGYKQQVFPREINFFFLKDNFR
                     GRIVKEDSMYKVLGSRESWTEGEFKEAIQKEPWKFSPNVLLRPLYQESILPNLAYIGG
                     PAEIAYWLQLKSSFDHYQVPFPILVPRNFCLLIKKDVEKKLAKTGMKIQELFQNKELV
                     IKNHVKKISKSNLTTSNEKSEVEKVWDELANRVKGLEPTLEITSLAFKAKSLKSLEVI
                     EKKMIRAEKRRQSTAVNQIEWIYGVLFPKNNLQERYDNYLTFLPDMPNLIEDLLIHLD
                     PLDFRFHILIQE"
     gene            12078..12635
                     /locus_tag="VGA21_03830"
     CDS             12078..12635
                     /locus_tag="VGA21_03830"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEX9649663.1"
                     /translation="MTKKEIRKKYLEKRKELGQAKIDLDKKILLKFFSSFSFIGNQTI
                     HIYLPMEKFNEVNTNLLIEELKRRGQRIVVPKIIGEEIISIEINDKTSYRETTWEIME
                     PLDGTEVKPEAVDVVIVPLLAFDTNGNRVGYGKGFYDRFLKKCRSDSLKIGLSFFPGE
                     ETIEDLSEADFQLTHCVTPDRIYEF"
     gene            complement(12623..13309)
                     /gene="mnmD"
                     /locus_tag="VGA21_03835"
     CDS             complement(12623..13309)
                     /gene="mnmD"
                     /locus_tag="VGA21_03835"
                     /inference="COORDINATES: protein motif:HMM:NF033855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016645 - oxidoreductase activity, acting
                     on the CH-NH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD"
                     /protein_id="HEX9649664.1"
                     /translation="MHKVEVIITKDGSSSLFVPSLDETYHSRHGAITESVHVYIQHGL
                     YERVESANLSTVDIFEVGFGTGLNTLLTILACLKRDITLNYTTIEPFPLDKATLDKLN
                     YHSLLSQPEANDWFNWLHNTEWDQIQSRGKISFIKTKDRLETFHTEKKFEIVYFDAFA
                     PEKQPELWKPEIYKKIYSMMNESGVLTTYCAKGQVKRDLLMAGFMVENLPGPPGKREI
                     IRATRPPQNS"
     gene            complement(13309..13788)
                     /gene="ispF"
                     /locus_tag="VGA21_03840"
     CDS             complement(13309..13788)
                     /gene="ispF"
                     /locus_tag="VGA21_03840"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015265334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HEX9649665.1"
                     /translation="MNIRIGIGYDVHALEEGREFWLGGIKINHYKGASGHSDADVIIH
                     AICDALLGALSLGDIGKYFPDTDPQYKNIDSKKLLVQVVEMVTSRGYIVGNVDVSLCL
                     EKPKVGPYLPGMIKTLCAILKIRETDVSIKATTSEGLGFVGREEGVEAHAVVLISKN"
     gene            complement(13788..15029)
                     /locus_tag="VGA21_03845"
     CDS             complement(13788..15029)
                     /locus_tag="VGA21_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020606948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX9649666.1"
                     /translation="MIQFENFTLENGLRVIVHEDPMTQIAVLNILYNVGSRDENENQT
                     GFAHLFEHLMFGGSENIPSYDEALQMAGGENNAFTSPDFTNYYLTLPSFNIETGFWLE
                     SDRMLSLSFDPHVLEVQRKVVVEEFKQRYLNQPYGDLWLKLRPLCYRVHPYRWPTIGK
                     EIEHIENATMDQVKSFFKKNYVPNNATIVVAGNVKTEEIRSLSEKWFGDIPAGNINGK
                     MLPQEPEQKEPRFLQIESKVPAHSITKAYHMPARFDPGYYPADLLSDILGRGKSSRLH
                     NQLVQVNQIFSSINAYLTGSLDPGLLVIEGKLNPGVEMESAEDEISKIINKLKSELIE
                     DSELEKVVSQAESTMVFSEVEILNRAMNLAFFDLTGDAGNINLELKKIKKVSAADIRE
                     SANYILKDENCSTLHYFSKPN"
     gene            15117..17090
                     /locus_tag="VGA21_03850"
     CDS             15117..17090
                     /locus_tag="VGA21_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009579208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase E1 component subunit alpha/beta"
                     /protein_id="HEX9649667.1"
                     /translation="MTFNRKGLTNQTLIALFTGLLKPRMIEEKMLILLRQGKITKWFS
                     GIGQEAISIGAVNAVESDEYILPMHRNLGVFTGRNIPLNRLFAQFQGKLSGFSKGRDR
                     SFHFGTNEFHIVGMISHLGPQMSVADGIALADKLSGVKKVTLVFTGDGGTSEGDFHEA
                     INVAAVWDLPVIFIVENNGYGLSTPSSEQFRFKSFTEKGPAYGIEAYSINGNNVIDVY
                     REVKKLATSIRDNPRPVILEALTFRMRGHEEASGTKYVPKKLMDHWAKKDPVINYQKF
                     LIEEKILDKEKIAEIRDSISKEIESGLKIAYEEAMPEPNSETELADLYKPFHDKITSP
                     NKKTSSKRFVDAVSDGLKAGLVKFPNLIFMGQDIAEYGGVFKVSEGFVEQFGKKRIRN
                     TPLCESAILGIGLGLSIKGFKSVIEMQFADFITSGFNQVVNNLAKSHYRWGQNSDVVI
                     RMPTGAGVAAGPFHSQSNEAWFFHTPGLKIVYPSNPFDAKGLLIRAIEDPNPILYFEH
                     KVLYRSIADEIPDDYYSIEIGKGKLIREGSDFSIITYGMGVHWAKRIVSEKNYSADIL
                     DLRSLMPWDKEAVEKTITKTGKVLILHEDTLTGGIGGEIAAWIGENCFRALDAPIMRL
                     GGLDTPIPFSPPLELNFLPDGRFRQKIEELLEF"
     gene            17158..19944
                     /locus_tag="VGA21_03855"
     CDS             17158..19944
                     /locus_tag="VGA21_03855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649668.1"
                     /translation="MISLISIFFSLRGAFKYLKGKARLIGFGLIAIFFLTGTGNLAAQ
                     DDRPAKPRKEKVKRSKPRGERSANKNPRVRETRFKMQMKKGDRPENRDLTGRRVKRNQ
                     RTKGPQINYSNYKPASRRTQYKNKPGFSSAPQRIRTASRKGEHSPRGDISGHRIKVSR
                     SPKPSNRLTFNAPNPNVVKRGKSREGRKTYRSTYVRSVSGQNRGKPPFSSAPNPYVAR
                     SKKSREGRTRYASPSIRSRTAQDARSRNSTSAGGVKPPKSTGGTILSRRPSTTVRTSR
                     TQPGDRAKSFSRKQGPVKSISGNIRNNRPSNPFAVANTKGKRSGDVARNRDITGRRLR
                     ENKRSPGKHITKVAPNPYLKRSERGESGVSNKKQLVKAGRTSKPGDHFAKTSFGGVRT
                     STRSGETATNRSASGKNIRTKNLRSGRPVFAGKQYPGYMTATKRGEGGFSGKSGKIRS
                     STRPRERAGVQPGGFKTATARGERSTNRDIAGKRLRARNKESRTTQIVGLSQKRTLSR
                     SGELMNNGGRSLESRPPGKGTLQGGTFSGTLRAGKGTNAGPGAKYSGNVRSFKPPKGG
                     GSITRLRNNKGNPLAARKPGKGTIAGGRYSGNISKGPASKPGKGAIHGGNFSGNLRSR
                     KGFYTTEKAYGLRTEKERLHNNKGNAIDQKMVGRGTIQGSAFRGKLSAKSNKPGKGAI
                     AGNRYSGNLRKGPATKPGRGTIQGGNYRGNLRSRKGFYTNERTYAVRTQIDERLHNNK
                     GNPLDGRQVGRGTIQGATYRGNLRADKSQKPGKGAIAGYRYQGNIRAKKDLERSEGTR
                     YKGRLSARTATPKIQPQGQEYRGKMRASRGYDHQKAGDALKQTRAWYQIGTLRADKYA
                     GNYRYKSPTRKDQHISSAFTGFNPGNAKKENDRFFKFKIWFAKLFRNETPRHLKNKGR
                     KPRYDRGESDIWYR"
     gene            complement(19947..20351)
                     /locus_tag="VGA21_03860"
     CDS             complement(19947..20351)
                     /locus_tag="VGA21_03860"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017731283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEX9649669.1"
                     /translation="MPIIESKYLGNLRCESIHLKSEQKLITDAPVDNHGKGEAFSPTD
                     LLCCSLGTCMMTTMGIKAQNFDLDIQGLSVELEKFMSSSPRKVAKAVIRMKWTNPVSD
                     PDLIKILKDTALNCPVALSLSDLLEQEVIFEF"
     gene            20435..21157
                     /locus_tag="VGA21_03865"
     CDS             20435..21157
                     /locus_tag="VGA21_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008198703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649670.1"
                     /translation="MKKTLPLILFLLFFAFHAFSQSGRQEFEYTREFTWGINKNTSGG
                     LIGGFVFKHSKAVSPRMFRTIGLEIVNIKHQKEVRYNSQFTGNFFIWGKENYLYSLRF
                     QYGRDLIIFRKAPQQGVQIMAISAIGPSIGILAPYYIEYLQGNNAIREQYDPDRHNFQ
                     NILGTGYVFQGLGESKLRAGANLKLGISFEFGTFKTSVTGFEVGFLADGYLGDLVIMP
                     KAPENRNLYPTAYISLFYGTRK"
     gene            21219..22106
                     /gene="lipA"
                     /locus_tag="VGA21_03870"
     CDS             21219..22106
                     /gene="lipA"
                     /locus_tag="VGA21_03870"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013454931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HEX9649671.1"
                     /translation="MIELPVVEKDGAHKKRKPDWLRVKLPIGKEYAHVRKVVDDHKLH
                     TICESGNCPNMGECWGAGTATFMILGNVCTRSCTFCAVATGRPPEYDEGEPQRVAEAI
                     RLMKVKHAVITSVNRDELKDRGAEIWYQTVVKTKQLNPETTIETLIPDVKATWDSLYR
                     MISAGQEVVSHNMETVERLYKRVRPQAKYERSLEQILKTKEYGKRTKSGIMLGLGETD
                     EEVFKAMDDLVAHGTDILTLGQYLQPTKMHIEVVEFIHPDKFAFFKEEGLKRGLKYVE
                     SGPLVRSSYHAERHVDVPV"
     gene            22168..24180
                     /locus_tag="VGA21_03875"
     CDS             22168..24180
                     /locus_tag="VGA21_03875"
                     /inference="COORDINATES: protein motif:HMM:NF012931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HEX9649672.1"
                     /translation="MNRIVIYILLLSFSKLSFAQPQLSLMPWPEEVVFGRGQMRLTSD
                     FTISVSQNATDKLCRYATRILRRIDGRSGMFFKQETVANDRVNPNAALLIQTTRPGLT
                     QLGEDESYHLRIENNQIFISAETEIGAMRALETVVQLLRVDSRGFYFPELTINDQPRF
                     PWRGLLIDVGRHFMPINVIKRNLDGMAAVKMNVLHLHLSEDQGFRIESKIYPKLHELG
                     SNGEYFTQEEIKEIIRYADDRGIRVMPEFDMPAHASSWLVGYPELASAPGPYEIETGF
                     GVKDPVMDPTSPVTYVFLDQFIGEMAALFPDPYFHIGGDENNGVQWNASQSIQKFMRE
                     NSIANNHELQSYFNEKILSFLQKYRKKMVGWDEIYQPGISKDIVIQSWRGKDALFESA
                     FNGYKTFLSNGYYIDLAHPAINHYLNDPLPEDHPLPDDKVGNILGGEATMWSELITKE
                     TIDSRIWPRTAAIAERLWSPGYIRDEREMYRRLDDISIDLEELGLMHLRNQDVLLRRI
                     ANGQQIHALKELIGLLEPVKGYERHNRGVSLTTHSPLTRLPDAAVPDSKPARDFRFLV
                     EDYLQSGEENLRQEIFNQLMFWKEIHKKLEPITVRAPLAEDALKYSRDIESLCAIGVQ
                     AVSVNMLHSQRWKDEVSMLLDAVNREKRECEIMIVGAIEALVKVAK"
     gene            24189..25343
                     /locus_tag="VGA21_03880"
     CDS             24189..25343
                     /locus_tag="VGA21_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018476750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9649673.1"
                     /translation="MIPRRILPAIVVSQFAGTSLWFAGNAVISDLQTSLNLPAESVGW
                     ITNAVQLGFIAGTLIFAFFSFADRFSPVRIFLLCSIIGGIFNLGILISENLFSLLAFR
                     FLTGFFLAGIYPVGMKIAASWYKESLGKALGWLVGALVIGTSFPHLVKGLHFSPDWQW
                     VIILVSIVAISGGLVIYFLVPDGPLTARTASFRFNVLAKAFQSRDFRSAAFGYFGHMW
                     ELYTFWAFTPVLIQYYAQQHPLPVSTPVLSFITIGIGGVGCILGGLTSIKKGSARVAF
                     AMLLTSGLCCLFSVFIIEFPTPLFFFFLLIWGFSVVGDSPQFSTLSAMTAPPEYVGTG
                     LTIVNSIGFAITIISISLVNSGSGFIDFKYLFLLLLPGPIFGLWSIRKLV"
     gene            complement(25493..25888)
                     /locus_tag="VGA21_03885"
     CDS             complement(25493..25888)
                     /locus_tag="VGA21_03885"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX9649674.1"
                     /translation="MLIGEVSKKTGFSKDTIRFYEKMGLIEISHRERRPNNYKEYSER
                     IIDRLQTIKNLKDFGFTLNETKELIQAWEMGIFDCSDEMPRMLDKIRSIENQIRNLKL
                     IKESLERSIQNCPEDCNIVNALEPISRGR"
     gene            25970..26365
                     /locus_tag="VGA21_03890"
     CDS             25970..26365
                     /locus_tag="VGA21_03890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649675.1"
                     /translation="MKQLFIIPVLLLSVNLMAQNKSTNMEKVSVKPRTMSCKLTSPEL
                     QERKITIIANLKAQILEKKEIDNGYTYKFSGTDAIIDELSQFIKTERKCCSFFDFTIE
                     IEGVEDGNIWLAITGPEGVKDFIKEDLDL"
     gene            complement(26568..29453)
                     /gene="gcvP"
                     /locus_tag="VGA21_03895"
     CDS             complement(26568..29453)
                     /gene="gcvP"
                     /locus_tag="VGA21_03895"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008624248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HEX9649676.1"
                     /translation="MSQNQHLSDNFVNRHNGPSEDEVAEMLKTVGAASLDELTEQTVP
                     RGIRLDRNLNTPEAQTEFEFLNHLKALAIKNKIYKSYLGMGYYGTITPGVIKRNILEN
                     PGWYTAYTPYQAEIAQGRLEALINFQTTVMDLTGMEIANASLLDEGTAAAEAMAMLQS
                     LRKGERQNSEEFFIDEEVFPQTIDVLKTRSKPLGVTLNIKSLDQLDVTSGSIFGIMLQ
                     YPNNSGKAIDYRSVIEAAHDKGIMVVVASDLLALTLLTPPGEIGADVVVGSSQRFGVP
                     MGYGGPHAAFFATKDEFKRQIPGRIIGVSVDSQGKPAYRMALQTREQHIRREKATSNI
                     CTAQVLLAVMAGMYAVYHGPRGLKGIATRIHSLTQALAKSLESLGFVNKNETYFDTLR
                     VGLPDGVNQSDVKKNAESAMMNFRYFENGDIGISLDETTNTADVSNILNCFSETGGNA
                     LRLDTIAEGTFPDSLTRQSDYLTHPVFHSYYSEHEMLRYIKKLENKDLSLAHSMISLG
                     SCTMKLNATTEMEPVTWPEFGNIHPFAPINQARGYTEMFSKLSEWLSDITGFPGVSLQ
                     PNSGAQGEYAGLMVIRSYHESKGEGKRNVTLIPSSAHGTNPASAVMAGTQVVIVKCDD
                     KGNIDLDDLKAKAEQYREQLVALMVTYPSTHGVFEESIKDVCKIIHDNGGLVYMDGAN
                     MNAQVGLTSPAEIGADVCHLNLHKTFCIPHGGGGPGMGPICVAEHLTPFLPGNPVIKT
                     GGEKAINAISAAPFGSSSIMVISYAYIAMMGRDGLKKATQLAILNANYMKERLADHFP
                     ILYTGKNNRCAHEMIVDCRAFKKFGVEVEDIAKRLMDYGFHAPTVSFPVPGTLMIEPT
                     ESESKAELDRFCDALITIREEVREIEQGNADPENNVLKNAPHTLEMSLKEEWDYPYSR
                     SKAVFPLDFVKESKFWPSVGRIDNAYGDRNLFCACVPVEEFKEGVSA"
     gene            29653..31170
                     /locus_tag="VGA21_03900"
     CDS             29653..31170
                     /locus_tag="VGA21_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009579217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEX9649677.1"
                     /translation="MKNKPILLLIAILFSALTGYAQADQSLIYSETINNDDMYDKLSI
                     IASDALEGRETGERGQKMAAAFIQYNFEKMGLIGPVKDSENPYFQNVPLVTSKPGDVW
                     LRVKGKDLKNFEDILYLGNASLDYKDKEVILAGNGQDADYEAVDVNGKSVIIYLGEGT
                     RRGVSNKAYENGAEFVLLVPAGNDDDFRNYIGRMSRFARGGGLSLDLPNREASKGAVF
                     ISSAIASDILGTSDDKIQKALASEDKNQLKKIPTGKFTFNSEMVTEKITSENVLGFLE
                     GTDKKEELIIITAHYDHIGRNGDQINNGADDDGSGTTSVIEIAEAFVAAKKNGNGPRR
                     SILFMTVTGEEKGLLGSQYYTANPVFPLKNTVVNLNIDMVGRVDPKHVENPDYVYLVG
                     SDRLSSELHEISEKVNETFSQLDLDYTYNDENHPERIYYRSDHWNFAKNNVPIIFYFN
                     GTHEDYHRPTDTIDKINFDMLVKRARLVYHTAWVLSNRDERLVVDKVQETTIDTR"
     gene            complement(31184..32431)
                     /locus_tag="VGA21_03905"
     CDS             complement(31184..32431)
                     /locus_tag="VGA21_03905"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009284892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX9649678.1"
                     /translation="MGFSNFKLNKQLLTAIAEAGYNLPTAIQTKAIPLIQAGHDVIGI
                     AQTGTGKTAAYLLPLLKKLNYAQGKSPRALILAPTRELVIQIEENARTLSAYTDLRMA
                     GIFGGKGIRQQIDLVKTGIDLLIATPGRFWDVYKSGELITKELKTMVLDEADRMMDMG
                     FAPQLRRIFEVVPAKRQNLLFSATMPEKVNAFCNEFLVFPFKAEVTPESTPAENVVQK
                     KLSVPNFKTKLNLLLNLLENENYSRLLIFVKTKQSANNIYKFLLRKKIGEVKVIHSNK
                     DQNFRLNAINQFSNGELRVLVATDVAARGIDVAEISHVINFDVPVQYEDYVHRIGRTG
                     RASAKGDAITFVSPADEYHIRKIEKLIKAKIPLLNLPSDIKVEMTPFEEQQVMNREID
                     KQKKSEDPTFRGAFHLKLKRKRK"
     gene            32508..33845
                     /locus_tag="VGA21_03910"
     CDS             32508..33845
                     /locus_tag="VGA21_03910"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009579227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEX9649679.1"
                     /translation="MVSDILILNGKIVNDGTITEKDILVRNEKIEQIAGDLSSIPAKK
                     IINAAGKIILPGVIDDQVHFREPGLTHKASILTEAKAAVAGGVTTYMEMPNTIPNTLT
                     QELLEQKYQIASQKSLANYSFFLGASNDNLEEVKKTDPNSVCGIKVFMGSSTGNMLVD
                     NEKTLEAIFREAQVLVATHCEDEGTIRKNMAAFTEKYGEEIPIEAHPLIRSHEACFKS
                     SSLAVELAKKYDTRLHILHISTAKELELFKRGPVENKRITSEACIHHMWFDDQDYATK
                     GTLIKWNPAVKTEADKIAIFQGLLDGVIDIVATDHAPHTLEEKQNKYLNAPSGGPLVQ
                     HSLVAMLEFYHRNKISLEQIVEKMCHNPAKAFQIEDRGFIKEGYFADLVVVDLDNPWT
                     VSKENILAHCGWSPFEGTEFKSNISTTLVSGHPAFENGMFNQSKTGRRITFDR"
     gene            33908..33983
                     /locus_tag="VGA21_03915"
     tRNA            33908..33983
                     /locus_tag="VGA21_03915"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:33943..33945,aa:His,seq:gtg)
     gene            34252..34506
                     /locus_tag="VGA21_03920"
     CDS             34252..34506
                     /locus_tag="VGA21_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014020114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649680.1"
                     /translation="MEEYQTFEKLSLHDKIKTLYVKGTFITSIRYYEYKINLYLFNGI
                     YLEVFYHHKKDQIEKIEQLQRSNTRMKFYLDQVKLCGLPI"
     gene            complement(34676..35290)
                     /gene="coaE"
                     /locus_tag="VGA21_03925"
     CDS             complement(34676..35290)
                     /gene="coaE"
                     /locus_tag="VGA21_03925"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEX9649681.1"
                     /translation="MIKHFQVGVTGGIGSGKTVVTKIFTVMGIPVYDSDSRAKQLMSE
                     DPELKSEIIRAFGDESYGVHGLDRKFIAAKVFNNPAELERLNQMVHPKVGRDYSKWLT
                     EQTSNIVINEAALLYEKDIAKRLNFLIVVTAPDIIRIERVLKRDNRTESQIREIISNQ
                     WPQEKSSLLADAVIVNDNTSALIPQVLDIYETIKRKASLQNKLA"
     gene            complement(35283..36239)
                     /locus_tag="VGA21_03930"
     CDS             complement(35283..36239)
                     /locus_tag="VGA21_03930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649682.1"
                     /translation="MVKNIWHGIKNIIIPDRVANWKVIILCIIGATTFWFFNSLNKSY
                     TTTISYPINLIFDESKYIAVSQLPSRVQINLTGGGWNLIRRTNWFNIEPIILVLEDPL
                     QRRVAGNSFRSSISDQLDEFQLNFIVTDSLALNIENKIDRKLKLKIDSASISLDENHY
                     LISSISLSVDSAIFTGPESMMTLLNNEFLVEIPEQAIDGDYLQMVIIKTGADHLFSVN
                     PPNVGVSFEVRRFLRVSKTVKSTLINFPADSSWYVRNPNVTISFMVREDRAATDSSAF
                     EIILDLSRMARADSTIQPLIRKYPGDVLDINIESGRVKVSHD"
     gene            complement(36257..36568)
                     /gene="yajC"
                     /locus_tag="VGA21_03935"
     CDS             complement(36257..36568)
                     /gene="yajC"
                     /locus_tag="VGA21_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013454046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEX9649683.1"
                     /translation="MIHPILAQANGQGSGYSSIIFFAAIALIFYFFMIRPQQKKQKQQ
                     KKFLEEVKKGDLVITIGGLHGKVLNVENDTIVLEVDKGLKLTFEKSSISMEASKKRQT
                     P"
     gene            complement(36671..37237)
                     /locus_tag="VGA21_03940"
     CDS             complement(36671..37237)
                     /locus_tag="VGA21_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009580839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HEX9649684.1"
                     /translation="MTRLLSATLFLAAVIFAGCDSKTSEKRIAELQDKLAEVENGGTA
                     TTGTATPASTTEAAAETTDSNIKPDGPLPAFEWIGGEEHDFGNITDGDVVQHTFLFKN
                     TGEAPLIIEKAQASCGCTVPAWTEEPVAPGATGEVQVKFDSKGKPNVQNKTVTITANT
                     YPKISRLRIKTFVNPKTSADAADGPVRN"
     gene            complement(37270..37575)
                     /locus_tag="VGA21_03945"
     CDS             complement(37270..37575)
                     /locus_tag="VGA21_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013454044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEX9649685.1"
                     /translation="MSNKSNAFLAFLLGAATGAILGILYAPDKGSNTRDKLTYRLDKY
                     KKTLEDMLEDLISGKDDLPKSKAKSEGEKVINDAKEKAEKLLQDVDNLIGHIRNENK"
     gene            complement(37583..38725)
                     /gene="nusB"
                     /locus_tag="VGA21_03950"
     CDS             complement(37583..38725)
                     /gene="nusB"
                     /locus_tag="VGA21_03950"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEX9649686.1"
                     /translation="MLNRRILRIEVLQTTFAYLQGKNSLKQMARESLAANLGPDMDTM
                     NNEDIRMLAVRQREALKIFDDKIEEVNPKCSHKVKEIEKLVNEAFIKYENDLKKDFKF
                     LKKRMLLDIEGTRTTYLRILQLLVEFKNVASTIKSNFGTPPENFVKNLLIRKIEYDNS
                     VQSAILASGISWTSHKEKVREWYRDIIRKDSQFDEYLKLDKTDFIADKKLIDHLLRDV
                     ILRNQVIGAFFEEEDLRWSEHEAVVKGMVKKSLKEIEEEKEFPRFSISMDWEDDKEFF
                     EVLFDRVVEEERNIEALIGRKVKNWELERLALIDKIILSLAVAELKNFPNIPVKVTIN
                     EYIEVSKRYSTPKSKEFINGVLDGITQELKSQGLIKKSGRGLLDNK"
     gene            complement(38774..39874)
                     /locus_tag="VGA21_03955"
     CDS             complement(38774..39874)
                     /locus_tag="VGA21_03955"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017731303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HEX9649687.1"
                     /translation="MEIKEAVEKSNSSLFEEVTRLGHEQVVFCNDPGTGLKAIIAIHN
                     TVLGPSLGGTRMWAYNSESEALNDVLRLSRAMTFKAAISGLNLGGGKAVIIGDARTMK
                     TEALMRRFGKFINSLGGKYYTAEDVNMKTRDMEYIHMETKYVTGLPKSMGGAGDPSPV
                     TAYGTYVGMKAMAKKVFGSDSLEGKKVMVQGAGQVGGYLVEHLLKENAIVYLSDIFEE
                     KAKAIATKGKVKVIEHDLVYETDADIYSPCALGATLNDETIPKLKCAIIAGSANNQLA
                     DEIKHGYMLMDQKIAYTPDFLINSGGLINVYLEYLGNHNYERAYQMADYIYDTCINIA
                     VFSDDNNVSPQEAAMEMANKRIKSIGNIKLTF"
     gene            40022..41812
                     /locus_tag="VGA21_03960"
     CDS             40022..41812
                     /locus_tag="VGA21_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009580844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9649688.1"
                     /translation="MKELSYLNKYLLKYKYHFILGIIFTIITNIFQIIPAQMVRHSLD
                     LIIANIDLYRNYAGLNLQNSYYDIFARYILIYGGIILLMALLRGFFLFLVRQTLIVMS
                     RLIEYDLKNEIYRHYQELPTSFYRRNSTGDLMNRISEDVSKVRMYLGPSIMYGINLIT
                     LFAILIPYMLTISVKLTLFALLPLPILSFSIYFVNNIINRRSEAIQKQQSKLSTFVQE
                     AFSGIRVLKSFVREEDSSRKFEEENTKYKNKSLRLTMVNSLFFPIVMALIGLSVILTV
                     FVGGNEVISGALTFGNIAEFILYVNYLTWPVTALGWISSITQRAAASQRRINEFMLVK
                     NDITSEKNIQQEIRGDIEFNGVSFTYPDSGIVALSSLDFKLNSGESLAIIGTTGSGKS
                     TVANLLCRLYDPTEGKITIDEINLKDYHLGSIRSQIGYVPQDVFLFSDTIFNNIAFGS
                     MEIPPEKVMQAARDADLYGNVMDFPDQFNTRVGERGITLSGGQKQRVSIARAIVRDPK
                     ILILDDSLSAVDTKTENTILKSMKLLMKDRTTIIISHRVSSAKLADKIIVLDDGVIVE
                     QGTNESLLKSDGVYRSLYEKQLLAEELLED"
     gene            complement(41813..42349)
                     /locus_tag="VGA21_03965"
     CDS             complement(41813..42349)
                     /locus_tag="VGA21_03965"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX9649689.1"
                     /translation="MRIVIMFILIGVFSLHAFTQKAGYVCPPCGCSNDGKVFKEPGKC
                     PACSMTLLNKDNKSEGLNYTNLDPEEVCELQSRVTNVLFLDVRTEGEFNRSPLGRIKD
                     AKHIHVEELGSRIKELAEYKNKEIIVYCLVGIRSARASEYLAENGFTKVNNMLGGMEL
                     WNQSRDETVKYKNTLLDR"
     gene            complement(42653..43213)
                     /locus_tag="VGA21_03970"
     CDS             complement(42653..43213)
                     /locus_tag="VGA21_03970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649690.1"
                     /translation="MKIICPVFIPNEYPYQGIGIKVGDPFAVTYKLYVSKFLAMEIAV
                     GHGASFVYKKFYQDNFSNFEFLDTLNYLSHSVKNDLVGQFRIMIHSEIKSLEGLDWYV
                     GGGYQFKNTKILYRYNDPDTNGFTMEQETRREQFFYGPDIFLGLEFANFAIPVSIFFE
                     GNMFLILEANGLGPRFQAVAGIRYIF"
     gene            complement(43400..45175)
                     /locus_tag="VGA21_03975"
     CDS             complement(43400..45175)
                     /locus_tag="VGA21_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010609726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9649691.1"
                     /translation="MFLEQENISGRVIDFQVLKRLLKFVGPYRFKFYLLIFLTLTLAI
                     LAPLRPYIVQLTLDNDVMKGDYAGLRFMMLILVGLLVVNAIVQYIHTYLSGWLGQFII
                     RDIRIQLYKHLLSLKLKFFDNTPIGRLVTRTISDVETLLDVFSEGLAAMIGDLLQLVF
                     ILGLMFYTDWRLALVSLASFPILIISTYIFKEKIKVAFNQVRNAVANLNSFVQEHVTG
                     MSIVQIFNSEERELQKFKNINRDHKRANIRSVMYYSIYFPVAEVIGAAGIGLLVWYGA
                     KGVINFEQTGITLGMLVAFIMYINMFFRPIRMIADRFNTLQLGIVSTSRIMKLLDSKD
                     YIPDEGTTQVDKIHGAVNFKNVWFAYNTTDYVLKDISFKVNRGETIALVGATGAGKTS
                     VINLLNRFYEINRGTITVDDINIRDFPLDNLRKNIGVVLQDVFLFSDTIRQNITLGNS
                     EITDEDIWKAADSVGAKKFIERLPGQLDYNVMERGSTLSVGQRQLISFIRAMVYDPKI
                     LVLDEATSSVDTETEEMIQEAISKMMEGRTSIVIAHRLSTIQNATRILVFEKGEIKEE
                     GSHEELLTKSGLYSQLHKMQYKEAG"
     gene            complement(45196..45951)
                     /gene="truA"
                     /locus_tag="VGA21_03980"
     CDS             complement(45196..45951)
                     /gene="truA"
                     /locus_tag="VGA21_03980"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017731309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEX9649692.1"
                     /translation="MRYFFEISYKGTNYHGWQIQKNALAVQQVFNESLSILLREPVET
                     LGSSRTDTGVHASQQFFHLDTTKIIEPEILISKMNSLLPGDISVKSIRPVKPNASCRF
                     DATSRSYEYHIIKRKDPFLSWTAVEIRKPLDVDAMNLAATYLIGKKSFKSFCRNSMAV
                     PGHDCDVSRAIWKENGDSLIFLITANRFLRGMVRSIVGTLIMVGDGSITSREFAKILN
                     SKDRKLAGPAAPAKGLCLTKVKYPPGIFKVKSS"
     gene            complement(45958..47157)
                     /locus_tag="VGA21_03985"
     CDS             complement(45958..47157)
                     /locus_tag="VGA21_03985"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007653018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEX9649693.1"
                     /translation="MKSAYIVDIIRTPIGKFAGSLASVRPDDLAAHVIIKLLERNQNV
                     DPSLIEDVIFGAANQAGEDNRNVARMALLLAGLKIETGGVTVNRLCASGLQAIMDASR
                     AVQLGEGKIYIAGGSESMSRAPFVMSKAESAYSRNVEIFDTSIGWRFANPKLVDLFYP
                     FSMGETAENVASQWKISRTEQDEFAHSSQLKYSLAHQGSKFDAEIVSVEILRKKEEVV
                     IFAKDEQPRLSSMQVLAGLRPVFKKDGTVTAGNSSGINDGAAACLVVSEETLKELDLK
                     PMARIVSVAIAGVNPDTMGIGPVPATRKALKRAGLKINDLDLIELNEAFASQSLACIR
                     DLELNPEIVNVNGGAIAIGHPLGCSGARISGTLIHEMQKRTKAKYGLATMCVGVGQGA
                     AVIYEKC"
     gene            47230..47712
                     /locus_tag="VGA21_03990"
     CDS             47230..47712
                     /locus_tag="VGA21_03990"
                     /inference="COORDINATES: protein motif:HMM:NF025491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4293 domain-containing protein"
                     /protein_id="HEX9649694.1"
                     /translation="MLQRIQTVFLFLVSLLMVVDVFSPIWININIETGESHILYSAFY
                     EFIPATGEPAQNTFFPYTIVSLTALIAAGIAFYEIFQYKDRLTQMKLGALNSIIMTVC
                     LGCSVWFATDGQQTWMPRNTGSYGYGLFLPAVAMILNIIANRFIRKDEKLVRSVDRIR
                     "
     gene            47803..49695
                     /gene="htpG"
                     /locus_tag="VGA21_03995"
     CDS             47803..49695
                     /gene="htpG"
                     /locus_tag="VGA21_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009580859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HEX9649695.1"
                     /translation="MAEKGTISINTENIFPIIKKFLYSDHEIFLRELVSNAVDATQKL
                     KRLSALGELKEDIGDQDLTIEVKFDKKKKTITVSDMGIGMTLDEIKKYINDIAFSGAT
                     DFLEKYKDKEAAQQFIGHFGLGFYSSFMVSKKVEIFTRSYQPGAKPAYWECDGTTEFS
                     ISDSEKKDRGTDIVLHIAKDSEEFLDEFRLKGILEKYCRFLPVKIKFGTKEEKVPDGK
                     DKDGKEKFKNVTVDTIINNPVPLWTRTPADLKDEDYLKFYKDLYPFQEEPLFWIHLNV
                     DYPFNLTGILYFPKIKNDFEGHKNKIQLYSRQVFITDEVKDVVPEFLMLLHGVIDSPD
                     IPLNVSRSFLQSDSNVKKINAHITKKVADKLSELFKKDRESFEKKWDDIAIFVKYGMI
                     SEEKFYDKAKDFCLLKNTESKYSTIEEYKKKVKDNQTDKNKELIFLYTTNPEKQDTYI
                     SAARKRSYDILMMNGAIDSHFINNLEQKLEKTHLRRVDSSTTDKLVDKDEKVESVLSE
                     EDTKKVKEIFEKAINNPSMILSVESHAPDEMPVIITQSEFMRRMSDMAASGGMGFMGN
                     MPKELNIVVNANHSIISKILKSRKEEKKTELAKQAYDLALLSQNMLDGADLTNFIQRS
                     VQLASD"
     gene            49761..50609
                     /locus_tag="VGA21_04000"
     CDS             49761..50609
                     /locus_tag="VGA21_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009578964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649696.1"
                     /translation="MKLNFKYLVGIPLTALSYLAFGQEDKKVDVEELFRIDTPITIEL
                     EEDEFERVEPKKKKQKRNTFFGIKTKKGFTKNGFGDNVVLELFYYLKDPIKIDPYVNE
                     IYWFDFRRKAIRAGKNVDPKYGVVLHGPYKKMKGKQVLEEGYYYEGAKHGRWTTYNND
                     NILITKTKYHKGWYKESLVSFYDLEHTQLREVIPVQYGKRHGEYFYFHPNGNIAVSGK
                     YENDVKIGVWTEFYEIRGRRKKMIQYTEDHYNKKFQPVTLKEWNDRGQLIYDRETYGF
                     GLPDTN"
     gene            complement(50606..50950)
                     /locus_tag="VGA21_04005"
     CDS             complement(50606..50950)
                     /locus_tag="VGA21_04005"
                     /inference="COORDINATES: protein motif:HMM:NF009150.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HEX9649697.1"
                     /translation="MSKTSAGKEGELLAINYLKSKGYDILRTNYRAGRNEIDIIARMD
                     SFVVFVEVKYRSTIKYGYPEEMVSGDQEERIREAAELYLETENWKGNIRFDIIAIHKK
                     KECAIFHIRDAF"
     gene            51086..52756
                     /gene="pgi"
                     /locus_tag="VGA21_04010"
     CDS             51086..52756
                     /gene="pgi"
                     /locus_tag="VGA21_04010"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015030736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEX9649698.1"
                     /translation="MKPGKISMLPKKNPTQTKAWKELKSHFQKVAPLRMVNMFKENPN
                     RAKRFTLQHDGLLLDFSKNRITEETMDLLIDLANEVQLKQAIQAMFTGAKINETENRS
                     VLHTALRKSTDNNLEIDGLHISEAVENVLQKVEKFSNKITTGNWKGFSGKRISDIVNI
                     GIGGSDLGPAMVCEALTPYKIKNINTHFVSNIDSSHLTETIKKVNAETTLFVISSKTF
                     TTQETMTNAWSARKWFLESATEADIAKHFVAVSTNKAATSQFGIEESNVFEFWDWVGG
                     RYSVWSAIGLTVACGLGFENFKKFLDGAASMDKHFLDKPFRENIPVILALISIWYVNF
                     FNCTSEAILPYDQYFDKLVDYLQQVIMESNGKSVDRNGERVSYNTCPVIWGAPGTNSQ
                     HSFFQLLHQGSQVIPCDFITTMKSQNPLGDHKRKLLANFFGQTRALMVGKSREEVSEE
                     MQRMGFTDSEIERIGPHKVFEGNRPSNSIIIEELNPYSLGMLLAMYEHKVFVQGIVWN
                     IFSFDQWGVELGKQIAKLILPALEKDNEISTDGSTQSLISHFKNIAKQ"
     gene            52757..53728
                     /locus_tag="VGA21_04015"
     CDS             52757..53728
                     /locus_tag="VGA21_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018341983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I phosphomannose isomerase catalytic
                     subunit"
                     /protein_id="HEX9649699.1"
                     /translation="MAEFYPLKFHPIFKSKIWGGQKIKTILGKDFGQLKNCGESWELS
                     CVEGNISIIANGIFAGRALDSLIQLYPNEIMGSRIKFENFPLLIKFLDANDDLSIQVH
                     PNDKLARKKHNGYGKTEMWYIIDADHGARMYVGFNRNLTREEFIQKAEAGEVTDWVCT
                     EEVMPGDTFYIPAGRIHSIGKGILLAEIQQTSDITYRIFDFNRMGDDGKPRDLHISNA
                     ADALNFNDVGTTFKTKYNATEPYAVLVKSPYFTTIRLTGEKSLKVNLNNENSFRIYIV
                     INGEGQINWAGNSMTISLGEVILIPASIREYELRTGMNFCGLEVYIS"
     gene            53731..54447
                     /gene="lipB"
                     /locus_tag="VGA21_04020"
     CDS             53731..54447
                     /gene="lipB"
                     /locus_tag="VGA21_04020"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009577729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HEX9649700.1"
                     /translation="MYTTKNKKVKIIDLGLMDYRESWQYQESVFKDVIDKKTKNRSLP
                     VNSQVLVPNFLIFVEHPHVYTMGKSGDEKNLLLNESQLKEKNAQYYKINRGGDITYHG
                     PGQIVGYPILDLDNFFTDIHKYLRMLEEAVIRTLMNYGLEAGRIDGLTGVWFDHISLK
                     NPRKICAIGVKSSRWVTMHGFSFNINTDLQYFNYIIPCGINDKAVTSLSKELGAKQNL
                     EEVKIILTKHLVDLFEMEII"
     gene            54451..55173
                     /locus_tag="VGA21_04025"
     CDS             54451..55173
                     /locus_tag="VGA21_04025"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012472353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEX9649701.1"
                     /translation="MNIQDLKNILLIDIETVGMAETFDQLHPRFQKEWERKSKFLLNS
                     DTMTPQELFEERAGIYSEFGKIVVIGLGLFNLSPEKELSLRVKALAGVDEKKLLEDFK
                     HLIESKFSADTLSLCAHNGKEFDFPYIIRRMLVHGITLPGAMDYSGKKPWEIRHIDTM
                     DLWKFGDRKSFTSLELLAALFGIDSSKNGIDGSKVNETFYKEKNLDKIIEYCKEDIVV
                     TGQVFLRLNALPSIKNENIHLL"
     gene            55194..57350
                     /gene="rnr"
                     /locus_tag="VGA21_04030"
     CDS             55194..57350
                     /gene="rnr"
                     /locus_tag="VGA21_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008202889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEX9649702.1"
                     /translation="MGRKKKKDHSNKKIKKVRRSDIANDIQQLLNSNLSSALSIKQIT
                     KKLGYRDKIQKNMVPETLFDLERSGSVQRLRNGSFKSTREAKLLTGVVDHVNPKFAYI
                     ISEESEDDIWVRTDDLNFAINGDTVSVQVYPQKHGKKPEGKVKQIIQRGKDEWVGRVE
                     ISKNYAFVIPDNKRIYCDVFIASEDINKARSRDKVIVKILNWPSAEKKNPKGEIIRVL
                     GPAGENEAEIHSIMAQFELPFEFPDKVLAESERIPEKISRDEISSRRDFRNILTFTID
                     PEDAKDFDDAISLQTLENGNTEIGVHIADVTHFLREDTVLDKEAFDRATSVYLVDRTI
                     PMLPERLSNGVCSLNPNEDKLTFSAVFEMDDKATVKRQWFGKTIIHSDKRFAYEQAQE
                     IIESGSGEFSDELVKLNELARKLRKDRFANGAINFETIEVKFRLDEKGKPLEVYPKER
                     KDAHKLIEEFMLLANKKVAEFIFKKKSGGNKSSTFVYRTHDSPDPDKIKAFSIFARKF
                     GYQMKLEESSISRSLNSLIDAIEGKPEQNVLESLAIRTMAKAKYTTEPDPHFGLAFMH
                     YTHFTSPIRRYPDVMVHRLLFLYLQNGKSPDEEEYEDKCEHSSEREKRAAEAERASIK
                     YKQVEFMQTRLGEEFDGIVSGVTEWGVFVEMIETKCEGMIRISEIRDDFYEFDEENFR
                     IVGVRNKRIITLGDDIKVKVVKTDIDRRTIDLEMIN"
     gene            57354..58316
                     /locus_tag="VGA21_04035"
     CDS             57354..58316
                     /locus_tag="VGA21_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017730089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEX9649703.1"
                     /translation="MLKEFQEAIEKEINSLTFQKPGELYEPILYIMQLGGKRLRPLLC
                     LLSYNLFAQEWKKILKPALALEIFHNFTLMHDDIMDNAPIRRGKQTIHKKWNSNVGIL
                     SGDVMLVKAYEHFTTVEGKYLLSVLNSFNKCATRVCEGQQMDMNFETQPAVSEKNYLS
                     MIEKKTAALLGFSMELGGILGGQNSEVTEKLYNYGRNLGLAFQLKDDLLDVFGDEQFG
                     KQSGGDIISNKKTFLLIKVLQKASGTDRKTLLYWLDQKEFDSEEKVKEVKAIYTRLKI
                     REEAESRINGYFDLATENLRYFKANKNAWELLFSFTGNLKSREK"
     gene            58313..58936
                     /locus_tag="VGA21_04040"
     CDS             58313..58936
                     /locus_tag="VGA21_04040"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013453641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEX9649704.1"
                     /translation="MSQTVILMIIIGLVSFVAWKQPAKMEKWILNPYKAFHNKEYFRF
                     ITSGFIHANWGHLIFNLFTLFFFGRNVEYTFIGLMGPATGILAFWILFITAVVVSDLP
                     SFFKFRNIPNYNSLGASGGVSAFVFSSIIFYPLQPIYIMFIPIGIPGFILGALYLLYS
                     YYQGKQLGDNINHDAHLFGALYGVLFTVIVFPQSISLFFEQIKNFSF"
     gene            59125..59952
                     /locus_tag="VGA21_04045"
     CDS             59125..59952
                     /locus_tag="VGA21_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013454627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9649705.1"
                     /translation="MYKLIVDANKPSFGLNFKELWAYRDLILILIYRDLRVRYTQTFL
                     GLSWAIIQPLATLLIFTMVFERAIGIDTGDIPYPLFALCGMSAWTYFAFVLNNSGNSI
                     ISSQEMIKKIYFPRLIIPLSKSMVGLVDFIIALLLIGCFMIFYGITPGNKFLYLSIFI
                     ALTIISALSVGIWISALTIRYRDLQHVVPFLVQFGLYATPVGYPSELIVKNLPDWAVT
                     LFYLNPMAGIVEGFRWTILGGSPPSVNVYYSFGVAILLFISGLYFFKKVERVMADII"
     gene            59949..61139
                     /locus_tag="VGA21_04050"
     CDS             59949..61139
                     /locus_tag="VGA21_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009194525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9649706.1"
                     /translation="MTDFAIKVRGLGKRFHTGKSKSADFRTSIGYQIKSIFRVGKMEK
                     ESFWALQDINFEIARGESLGIIGCNGAGKSTLLKILSRITAPTTGMFEINGRVSSLLE
                     IGTGFHMELTGRENIYLNGTILGMKRKEIRKKFDEIVSFSGVEKFLDTPVKHYSSGMK
                     VRLAFSVAAHLEPEILIVDEVLAVGDAAFQKKCLGKMSEVTRNEGRTILFVSHNMNAL
                     KSICSKGIVIRNGKIDLISDISNSINRYSANEETLTFSRTLKNAFIGDGIYIEDFTLS
                     KHTFEVFSDMEIELKMRFLEENMVSELALLINNETGERVAIIDMREYLEGKKTRSLEF
                     QGVINKMPLLEGHYSIGLYLLSNLATGNYQKLIKFSITGKETKIPSFPLNVRGYVELE
                     NKFN"
     gene            61140..>61421
                     /locus_tag="VGA21_04055"
     CDS             61140..>61421
                     /locus_tag="VGA21_04055"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HEX9649707.1"
                     /translation="MEKYFSQFGQDEYLDKKIFKKREKGFFIDIGAHDGITYSNSLFF
                     ERYRKWAGICIEPNPDVFLEMAKLRSSLNIEACIGTKHGEVDFLKVSGYA"
BASE COUNT        18761 a        12168 c        12369 g        18123 t
ORIGIN      
        1 gtaggagtac ccatatttcc tggctatttc cttttttgta atttcttcca gtgttatgat
       61 catgataata tcggttgtcg gacgttccga ctgatccgtt ggcactgccg caattttatc
      121 aatcacatcc aagccttcca caaccttgcc aaaaacagaa tattcaccat ccagatgaag
      181 tgcccctcca ttcttagtgt aagcttcaat ttgccgggat gaaatttctg gaaaagtctg
      241 tttaccatac tcctgctcta taattgacag cgactcaaat gttttacgct tcagtgcttc
      301 catgtcgccg gcttgctgca atgcaataaa ctcctgtaat aattccgggt tcttcccttc
      361 ataaaacatc ctgtttagaa gccccaccat tttcccatat tttacattat ctgcattcag
      421 ctgatcaggg gcataggttt gcccctggac aatatagaac tggctgccac tggattcttt
      481 ccctggattt atttgatcac taagtctggc tgcagacaat gctccttttt cgtgaaaaaa
      541 tccggttcgt atttcagccg gaatcgtata tcccggccca ccggatccca atctttgtcc
      601 ccggcctgca ttttttgaat ccggatcacc accctggatc atgaaaccct ggattaccct
      661 gtgaaaaata aggctgtcat aaaatccttc cttgaccagt ttaacaaaat tttctttgtg
      721 ttgaggagtc tcttcatata atatggcctt catatctccg tattccgtgt gtatcacgac
      781 caggtaatct ttttctccgc aggcactcat taataaaagg gttaatgtga ataaaagtaa
      841 atatctgaat ttcataagtt ttaacttttt acagttttta tctgcttaaa caatgatgca
      901 aacctcaaag ataagtctaa atatacaatt ctcgcattgg cgtttctctc caggtaaaca
      961 attgccgact ccaactcttt tgacatttcc tcaatcagtt cccagtcaat aatttttgaa
     1021 aaatcccgga tgaatgcttt ctctgactcg atggcaagct ggattgagct gctaaaacca
     1081 attaccaaag actcgcgcag catattcagc ccgtattcaa ataaactctt ttgagaaatc
     1141 ttcgatctct tcgaaaaggt atcgctccca ttcaccattt ccattacatt aaattcaaaa
     1201 caagctcgca tccatatgat caatcgtgta tgattatcat cctccagatt ttcttccagc
     1261 ttaaatgcca tattcagact tccatccgcc aggtgagata tttgtccggc cttttcagca
     1321 cccattccaa tatcaaccag gaattgagca atctctttgt caaaaaatgg acgaacattt
     1381 attgagatac aacgcgaaag tatggtggat ataacctgcg aagcctgatt ggatacaaga
     1441 atgaaaagtg tttttggagg gggttcttcc agaattttta atatgccgtt tgcagctgaa
     1501 atgtgcatca actccggaag ccagattagc atagtcttaa agttaccttc aaaggaaacc
     1561 agggttaaat ccttaattat ttctctgctt tcctcccggt aaataattgc ttttttattt
     1621 tcaccaccaa aaaaagatac ccagtcttca taaaccggat aaggattgga tggcaggaaa
     1681 ctacgccatt ccttaatata tgccttgctt ataggatcta tctttttgcc atccttggtg
     1741 ttaagaatgg gataaacaaa gtgaacatca ggatggacaa gttttcgatt tttttggcat
     1801 gaagggcatt caccgcatga atcagaatcg gttctttttt cgcaatataa atagctgata
     1861 tatgcccggg caagggcaag gtttgctcca ccttcgattc cggcaaagat ctgtgcatga
     1921 gcaaccttct cctcttttac ggattttaac agggattttt ttacttcagg aagacctggg
     1981 atttcctcaa atctcatcta cttgccacaa tattttctca tataattcaa tgcccttctt
     2041 tctcccaatt ctcttgatct cccgaaatca tcgcaagctt ctccttcacg acccatgttg
     2101 gcaaatgcag aaccgcgcat taaataaact tctttcaatt catcggtaat ttttgccatt
     2161 tcctctttag aactattatc tctgattttt ttaattaaca aattaataga ctccgagaaa
     2221 gcattaatag aagaccggaa atttccataa ttataaaatg cgtgtgccag gtttctgtag
     2281 tattccggat tttcaggatc caatgaaatc gctttgcgat agtcaagggc ggatttctca
     2341 taattttcca gcaccaaata tagcaggccg cgatcagaat aaaacctggc attatcagga
     2401 acaagctcaa tagcgctgga gtagtcctgc aaagcctggt ctcgttcttc cagtttcaat
     2461 ttgtaacccg cccttaaata gtagagtctg ccgttgcgcg gttcaacggc aatagccctg
     2521 ttaatgtcat ctatagccag gtcatattcc cggttaccgt tataagtgtt gattgaggaa
     2581 gctctgatac gcgcccgttc tacataatac cgggattgtt ttctatctaa agtaattgct
     2641 tctgataaat cacgaaggca gtaatcgcgg tgatatccga gatcagaata aaccatcgcc
     2701 cttcggtaat aggcttcagc ccttcccgga tctattctga ttacatttga aagatcttct
     2761 atagcacctt caagattctg aatttctgtt ctgacggtcg ccctcaacaa ataagcatcg
     2821 gcatatttag gagatttcag caaagcgcta tcaagagtta tcagtgcaga aaatcgatca
     2881 ccttccctaa gattatccct cgcaattgca agaaattccg cacctttatc ctgagcttta
     2941 actgaatcaa acttgttacc aatcaaaaaa gtaaaggcaa gaatcaggta tatgttccct
     3001 gaatggggtt tcatgtttgc aaaggtcaga aacctttaat atttaaacaa ccaaaagata
     3061 aattatcctt tttttgataa cttgccaaat agcatataaa taaccattga ccgcattttg
     3121 gaaattgtat ggttcatttt agggatagtt ctcggtggcg tagtttcatg gttcctggca
     3181 acttatcgat ttaaaagtaa tctgagttca aacgaaactg ctgtaaaagt ttacgatgag
     3241 cggatcaagg atatgatcgc tctgcttgaa aatgcccgcg atgaggtgaa acgtgaacgt
     3301 gacaaggtga tcactctttc gaacagactt tcaggcaatg aagccgacta tcgtaacctc
     3361 aaagaaagat ttgaacaaca aaaagaagag gttgccaaat tgcaggaaag gtttagcatt
     3421 gaatttaaga atctggcaca ggatattctc gaagagaaat caagaaagtt tacaatccag
     3481 aacaaggaaa atctggaagg tattctgaaa cccctgagtg aacgcattat cgagtttgaa
     3541 aaaactgtgc agcagaattc caaagaaagt attgaatgga attctgctct gcgtgaacaa
     3601 ataaaatcgc tgaaagaact gaacatccag gtgacaaagg aagccgagaa tcttaccaaa
     3661 gccctaaaag gagataaccg ggccatgggc gcctggggtg agattatcct ggaaagcatt
     3721 ttggaaaatt ccggactggt aaaagacagg gagtatattg tgcaaagtgt gtttgttcat
     3781 gatgatggac gccggtacca gccggacgtg gtaatcaacc ttcctgatga caagcatttg
     3841 gtaatagatt ccaaaatctc actggttagc tacgaaagat ttgtgaatgc tgaaacaaaa
     3901 gaggatacga aaatagccat taaaagtcac ctgacctcca tacgttccca catcagggat
     3961 ttaagcagta agaactatca gactttgtat ggtgtcaaaa gtcttgattt tgttctgatg
     4021 tttattccta ttgaatcagc cttcagtctt gcggtccaaa atgattctta tttattcaat
     4081 gaagcttatg atagaaatat tgttttggtg agcccaacca cgttgattgc aaccctgcgt
     4141 accatcgcta acatctggaa gaatgaatac caaaacagaa acgccctgga gattgcccgt
     4201 cagggaggtg agttgtatga caagtttgtt aacttcactg aagatttgtt gacactgggc
     4261 aagcacctcg accagacaca aaaacactat agctctgcca tgaaaaagct ggtggaaggt
     4321 cagggaaatt tgatttcaaa agctaccaag attaaagaac ttggtgctaa aacccgtaaa
     4381 tccattgatc aacggctcat agaacgatcc gaagattaaa gtaattcata acttattatt
     4441 tatgagtaac caggcaaagc ttcaaaaact tatcatcgtt ggagacaggg ttttaataaa
     4501 accaaaaaag atgtcggagc ggacatcaag cggattatat cttccacccg gcgttcagga
     4561 aaaagaaaaa atccaaagcg ggtacataat gaaagttggc cctggctacc cgattcccgt
     4621 gccggccgaa gaggatgagg catggaaaga gaaagacgag caaatcagat acatcccatt
     4681 gcagaccaag gaaggtgacc tggcaatttt cttacaacgc ggtgccatcg aaattatgta
     4741 tgaaggcgtt aagtatctta tagtaccgca gtcttcgatt ttgatgctgg aaagagacga
     4801 ggaattattt agctagtttt ggatcataat ggagcctgct tctgttaatt cgggaactta
     4861 agattttgat aaaagaggta ttttcacaat tgaattatcc aaacatctca tcaaaggtat
     4921 cctcaattat ggcactgcaa tccaaaatct gatctttagc aatgatcaaa ggtggtgcaa
     4981 accgaataat atttccatga gtgggtttag caagcagtcc tttttctttc aacttcatgc
     5041 aaatattcca ggccgtatct ccagcctcac tgtcattgat aaccacagca ttaaggagcc
     5101 ctttaccgcg caccagttta atgatatcga atttatcaac caacatttcc atccggcttc
     5161 tgaaaatttc acccatcttt tgagcatttt gggaaagatt ctcatccttc actatttgca
     5221 aagcttccat cgtaaccgca cacgccagag gatttccacc gaatgttgat ccgtgctcac
     5281 ctggctttat agtgttcata atggcatcgg aagcaagaac caaagacacc ggcatcatgc
     5341 ctcccgaaat tgctttcccc agaattaata tatccggatg gacattttca tgatcgcagg
     5401 caagtagttt tcctgttctg ccaatcccgg tttgaatctc atcggccatc aatagcacat
     5461 cataccggtg gcataattca gcaacttttg ccatatatcc atcagatgga acgtaaactc
     5521 cggcttctcc ctggatcggt tcaacccaga aaccacaaac atttgaattc tgcaaagcac
     5581 cctccagtgc tgagatatca tcataaggga taatctcaaa ccccggcatg aagggtccaa
     5641 aattagcgcg cgccaccggg tcggtagaag cggagattat ggtcgtggtt cgaccatgaa
     5701 aatttctccc aaccgcaaca attactgcct tattggccgg aattcccttc accatataac
     5761 cccattttcg ggcgagtttt attgcagatt cattcgcctc cgcccccgaa ttcataaata
     5821 gagccttatc gtaaccaaaa tactcacaga caaacttttc agtcaaacca agcttatcat
     5881 tgtaaaaagc tcttgaagtt agagtcagtt cccgcgcctg gttaatcaga gctttaacca
     5941 atcttggatg acaatgccct tggtttacag cactatatgc tgaaagaaaa tcatagtatt
     6001 ttttgccttc cacatcccac aagaagactc cttctccttt tgataaaaca acaggcaagg
     6061 gatgataatt atgacacccg tacttgtctt ccagttcaat agcttgctgg gctgatattt
     6121 ctaattctag catagtatta gcgcgattta tggtgataaa aatacgaaat tggcaatcca
     6181 tagcataata tgcggggtct gagtttgaga taattgaaaa agtttcgata tttgcactcc
     6241 taaaaatctt tgtgatgtca agagtttgtc aagtttcggg aaaaaggcca atggtgggga
     6301 ataaagtttc tcatgctaat aacaagaata aaagaaggtt ttacccaaat cttcatacca
     6361 agagattcta tattcctgag gaagaccgat gggtaactct taaggtttcc gcctccacca
     6421 tgaggactat caacaagaat ggcatttcaa aagttttacg ggaggccaaa aagaatgggc
     6481 tttatactgg aaaataggtt ttaatttagg agataaatag aaaatggcaa agaaaggtaa
     6541 cagagttcag gtaatccttg agtgcaccga gcataagaac agcgggcaac ccggtacatc
     6601 ccgatatatt actaccaaga ataggaaaaa tacccctgac aggatggaat taaagaagta
     6661 caatccgatt ttgaagaagt ataccttaca taaagaaatt aagtaaatca tggcaaagaa
     6721 agttgttgca accttaaaga ataaagaagc taaaaacttc gccaaggtaa tcaggatggt
     6781 gcgttctgaa aaaaccggag cctacacttt caaagaggaa atagttcctc aggaggatgt
     6841 tcaaaaagta ttggctaaaa aataaaggcc agcctaaaat ttttgttcaa agtcccgctt
     6901 cgggactttt ttgtttgttg caggttgcaa cgactgtgtt aaacccataa ttttattgac
     6961 aggcaaaaac aatggctgca ttcaaaggat tattttcacc ggataaaaag gaaacgcttg
     7021 ataaaggtct ggaaaagaca aaagaaaatt tccttacccg cataagtaaa gcagtactcg
     7081 gaaaatctgc tgtagatgag gaggtattgg ataaccttga ggaaattctt atttcttcag
     7141 acgtgggagt tcaaaccact atcaaaatta ttcagaagat agaagaaagg gttgccaggg
     7201 ataaatatct taatgcttca gagctggaca agattttacg agaagaaata ggaaagctcc
     7261 tggaagaaaa taataccggt gatttaacag atttcgaaat tccaaaaaca gcaaaaccct
     7321 atgtaattct tgtggttgga gtaaatggag tcggtaaaac tacgacaatt ggcaagctgg
     7381 cagcccaatt taagagaatt ggaaaatctg tggtactggg ggcggctgat actttcaggg
     7441 cggcagcggt agaccagtta aaacaatggg gaaacagagt tggcgtacct gttgtcgcgc
     7501 atggtcagaa tacggatccg gcatctgtgg catacgatac tgtgaaaact gcaatggacc
     7561 agggagcgga tgtcgtaatc atcgataccg cggggaggct tcacacgaag ataaatttaa
     7621 tgagtgaact ttctaagatt aaaagagttg ttcagaaatt cagaccggat gctccaaacg
     7681 aagtgttact ggttctggat ggcagtaccg ggcaaaatgc agttattcag gcaagagaat
     7741 ttacaaaggc taccgatgtt accgccatag ctattacaaa actcgatggc acttccaagg
     7801 gaggtgttgt tattggaatt tctgatgaat ttaagattcc ggttaaatat ataggtgtag
     7861 gtgaaaaacc ggaagacctg caagtattca ataaagctga atttgttgat tcattgttct
     7921 cagaaaggaa ttaatttact gactgagttg cggcctcaac ggctgccttt tttctttcag
     7981 gcttaggtgg aattggcttg ttcctgaatg gaattctaaa aaacaagtga taattgattg
     8041 cgaattctgt ccggctaatt ccacggccat accccggtac cagtagtgat tccaggctgt
     8101 tgctggtctt catgttcctt ccgaatttca ttcttcctgt cagacccaat tggacaaact
     8161 tccaaatcgg cactttcaat cccatcacca tttcgtacca ggtcgcgtac aaatctttat
     8221 tctcccttat aacccttcca tttccaaagt agtaggaatt gaaatcgaat tgcaccttgt
     8281 catcgaaggc cgcttccgca taacgaagac caaaaaaaac gacactcctg cttttttcat
     8341 atggagttag attaatatca agtcccaatc tccaaaaata tcctttggat gaatagtcgt
     8401 atatcacgtt cgattcactc ccttcaaaga taccatagtc aaaggagaaa aaatatttat
     8461 gaaaatctac atccgcatta aattcatata tggtgcgatt cgattttacg gttgtaagaa
     8521 cgatatcgta taaatctacc cctactctaa ctgcacttgg aacccatttg ctttgagcaa
     8581 atacagttac cggaacgcct atcaggatta ggaataaaat tctaataatc ataaatccga
     8641 acatttacat cgataccttc taagattcgc gtgttcacaa gtcttacaga gtcatatgaa
     8701 gtgccccaaa ttgccagatc agagaacaca aactcaggtc cacactcatc tgaattgata
     8761 aaaactgcgt ttctgtatcc cagcgtgatc caattttcta cgccggattg ggtcaccagg
     8821 taagtaacat tgtcccggaa gggatcgagg ggtagattaa attgggtagt aatctggttc
     8881 gccgtaacca aaatgctgtc agaagcagcg ctggtaattg taagtatttc gtttttgggt
     8941 actcccaggg aatcaacaaa ttgaattatc acaaaatctc tggataccgg agtgcaattt
     9001 tgtgcccttc tgcatgaaac gaaaacaatc aacaacaatg caagggtgaa tgctaatctt
     9061 attataaggc tttcttttct caatccttca aaattcaaat ataagacact ttgaaaccaa
     9121 acgagtaatt cccttatttc ccttcctgaa tgccaaaata aactaatttt gcagattcat
     9181 agagaagagt tgaaaacaaa ggcaagaaaa aataaagtaa atattgttac cctgggatgt
     9241 tctaagaacc tggtggattc agaagtatta ttaacacagc taagagggaa caagatagat
     9301 gtgactcatg aaaaccagga agacgatgct aatatcatca taataaatac ttgcggattt
     9361 attgacaacg caaagcagga atcggttgat acaattatcc ggtatgcaga tgccaagaag
     9421 gaaggtctga ttgataaagt ttttgttaca ggctgtctgt ctcagcgata taaggaagac
     9481 ctggaaaggg aaattccgga agttgatgct ttttttggca ccagggaact tccacaatta
     9541 cttagaagat ttaaagccag ctacaagtat gagttgttgg gtgagcgtac tacaactaac
     9601 acccattacg ctcacatgaa aatttctgaa ggatgtgaca ggccttgttc attttgcgca
     9661 attcccttaa tgcgtggaaa tcacattagc agaccgatag aagaattggt cacagaagcc
     9721 cgtaacctgg ccagaaatgg agtaaaggaa ttattattga tcgcgcagga ttcaactttt
     9781 tatggaattg atctttatgg caaacgaaag ctggcagagt taatgagcag gctgtcggat
     9841 atagatggtt tggaatggat tcgtttgcat tacgcatttc caaccgggtt tccaaaagac
     9901 gtactgaaag tgatggcctc aaaagaaaat atttgcaact atcttgatat tccgcttcaa
     9961 cacggctctt ccaaaatgct gcaattgatg cgaagaggca tcacccgcga aaaaacagaa
    10021 ttgcttctgc aggaaattag agccacagtc ccggagattg ccatacgcac caccctgata
    10081 gcgggtcatc cgggcgaaac tgaatttgat tttgaagaaa tgaaaggatt tgtggaaaag
    10141 tcaagatttg aacgtttggg cgtattcacc tactctcatg aggaaggaac tcattctttc
    10201 tccatgcagg atgacgtacc ggcagccgta aagcaagtcc gggctgatgc tataatggat
    10261 ctccaggaaa aaatcagctt tgagttaaac caggctaaag ttggcaaaga atttaaagtc
    10321 cttattgaca gaattgaggg agcgaattat gttggcagga ctgaatatga ttctccggaa
    10381 gtagacaatg aggtaatcat ctcttcggaa aacaattatc tgaggattgg cgatttttcc
    10441 atgattaaga ttacgagagc tgaagagttt gatttatttg gaatacctgt tcaaccggca
    10501 taattattat atttggcgat gcgtgttgag aaaatagaat tggtgaagac aaatcggttt
    10561 tcgcaaatcc tgattaatta tctgaagcaa gatgatgtct tccttgaaca attttatgac
    10621 ctctaccctt ccatcaataa ctttgaaaag caaatcaggg caaaaatatt tgccaaatct
    10681 attcgtgatg aattgttcca atcactaacc gagcaatatg aaggttatca gctaagtgat
    10741 aaagctcaaa taaatcttca aagccttaag aaagaaaata cttttactat tactacaggg
    10801 catcagctta gtatttttac gggcccgctt ttttttcatt ataaaataat tactgtaatt
    10861 aacctatgca ggaagctttc ccaaaaatac ccgaactata attttgtacc tgttttttgg
    10921 atggcgacag aagatcatga tttcgatgaa atcgcaaagg tgaatatcgg aggcatagat
    10981 attacgtggg ataccgtaca ggcaggtccg gtaggaaagt ttgatcccac cggcttaaaa
    11041 gctgtgctgg gtaaaattcc ggccgatgtg ggtatctttc aggagagtta tttgaatgaa
    11101 tcaacgcttt caaatgcagt acggaattac gtagatgaga tttatggaaa ggaaggtttg
    11161 gtagttattg atggtgatga taaaaggctt aaaactgcaa tcagcgattt aattattgaa
    11221 gactgctgca gcaataatgt taacagatta tccgaagctt cttccagtga acttgaaaaa
    11281 ttaggttaca aacagcaggt atttcctcgg gaaataaatt tcttttttct gaaagacaat
    11341 tttaggggca gaattgtgaa agaagactcc atgtataagg tgttgggctc acgtgaatcc
    11401 tggactgaag gagaatttaa agaagctatt caaaaagaac cctggaaatt cagtccgaat
    11461 gtgctattaa gacctttgta ccaggagtct atactgccta acctggctta tattggagga
    11521 cctgcggaaa ttgcatactg gctccaatta aaatcttcgt tcgatcatta tcaagtgccg
    11581 tttccgattc tcgttccccg gaatttttgt ctgctgatta agaaggatgt agaaaaaaag
    11641 cttgctaaga ccggaatgaa aatccaagag ctctttcaga ataaagaatt agttattaag
    11701 aatcatgtta aaaaaatatc caaatcaaat ctaaccacat ccaatgaaaa gagtgaggtc
    11761 gaaaaggttt gggacgaatt ggcaaacagg gtgaaaggtt tggaaccaac tttggaaatt
    11821 acctccctgg catttaaagc aaagtctctg aaaagccttg aggtgattga aaagaaaatg
    11881 atccgtgcgg aaaaaaggcg acaatcaacc gcggtaaatc agattgaatg gatctacggc
    11941 gtactcttcc caaaaaataa tcttcaggaa cggtatgaca attacctgac ttttctgcct
    12001 gatatgccca acctcattga ggacctttta attcatttgg atcctctaga tttcagattc
    12061 cacattttaa tccaggaatg acaaaaaaag aaattcggaa aaaatacctg gaaaaacgaa
    12121 aagaactcgg acaggccaaa atcgatcttg acaagaaaat tttactgaag ttcttttctt
    12181 ctttttcttt catcggaaat caaacaatcc acatttattt acctatggaa aagttcaacg
    12241 aggtgaatac aaatttgctt attgaggaat taaaaaggag aggtcaaagg attgtagttc
    12301 caaaaattat cggtgaagaa ataataagta ttgaaattaa tgacaaaaca agctacaggg
    12361 agacaacatg ggaaattatg gagccgttag atggcactga agtgaaacct gaagccgttg
    12421 atgtggtaat tgttcctctt ttagcttttg ataccaacgg aaacagagtg ggttatggaa
    12481 aaggattcta tgacaggttt ctgaaaaaat gcaggtcgga tagtttaaaa attggtctga
    12541 gtttttttcc tggtgaagaa acgattgaag atctttctga agcagatttt cagttaaccc
    12601 attgtgtaac ccctgaccgc atttatgaat tctgaggggg cctggtcgcc cttataattt
    12661 ctcttttccc gggaggtccc ggcagatttt caaccatgaa cccagccatc agcaaatctc
    12721 tttttacttg accttttgcg caataggtag ttagtactcc tgattcattc atcattgaat
    12781 aaattttctt gtaaatctcc ggtttccaga gctccggttg tttttcaggt gcgaaggcat
    12841 cgaaatacac tatttcaaac tttttttcag tatgaaaagt ttccagcctg tctttggttt
    12901 ttatgaaaga aatttttcca cgcgactgaa tttgatccca ttccgtgttg tgaagccagt
    12961 taaaccagtc atttgcttca ggttgggata gtaaagaatg atagttgagc ttatcaagtg
    13021 tagctttatc aagaggaaaa ggttctatag tggtgtaatt caaagttata tctcttttca
    13081 ggcacgcaag aatggtgagc aatgtattaa gtccggtccc aaacccaacc tcaaaaatat
    13141 ctaccgtcga taagttggcc gattcaaccc tttcatacaa accatgctga atataaacat
    13201 gtactgattc tgttatcgct ccatgccgtg agtgatacgt ttcatccaac gagggaacaa
    13261 atagggatga cgatccatcc ttagtaatta taacctcaac cttatgcatt aatttttgga
    13321 gataagtacc accgcatgag cttcaacacc ctcttccctg ccgacaaatc ccaatccttc
    13381 agatgtagtc gctttaatac taacatccgt ttccctaatt ttcagaattg cgcaaagtgt
    13441 tttgatcatc ccgggcaaat aaggccctac tttgggtttt tcaaggcaaa gagaaacatc
    13501 cacatttcca acaatgtacc cccgtgaagt taccatttcg actacctgaa ccaataattt
    13561 cttactgtct atattcttat attgaggatc agtatcagga aaatattttc caatatcacc
    13621 caggcttaga gcacccaata atgcatcaca aattgcatga ataattacat cggcatctga
    13681 gtggccgctt gcacctttat aatggtttat ttttattcct ccaagccaaa actcccgccc
    13741 ttcttccaat gcatgaacgt cataaccaat acctattcga atattcatca attcggtttt
    13801 gaaaaataat gcaaagttga acaattttca tccttcaaaa tataattcgc cgattctctg
    13861 atgtctgcag cagaaacttt cttaattttc ttcagttcta aattaatatt tccagcgtct
    13921 ccggttaaat caaagaaagc cagattcatg gcacggttca gtatttctac ttcggaaaat
    13981 accatagttg attcagcctg actcactact ttttccaact cagaatcttc aatgagttcc
    14041 gattttaact tatttataat ttttgaaatc tcgtcttccg cagattccat ttcaactcca
    14101 ggatttaact tgccttcaat cactaacaaa cccggatcca gtgaacctgt gaggtaagcg
    14161 tttatagagg aaaaaatttg attaacctga acaagttgat tgtgaagcct ggaagatttc
    14221 ccgcgtccca aaatatcact taataagtct gccggataat atcccggatc aaaccgtgcc
    14281 ggcatatggt aagcttttgt aattgaatgg gcaggtactt ttgactcaat ttgcagaaac
    14341 cttggttcct tttgctccgg ttcctgtggc aacattttac cgtttatatt tcccgcggga
    14401 atatcaccaa accatttttc acttaagctt ctaatctctt ccgtttttac atttcctgcc
    14461 acaactattg tcgcattatt tggtacgtaa ttttttttga agaaactctt cacctgatcc
    14521 atagtggcat tctcgatatg ctcaatctct ttaccaatag tcggccaccg gtatgggtgc
    14581 acccggtaac aaagaggtct caacttgagc cataaatcgc cataaggttg gtttagatac
    14641 cgctgcttaa attcttcaac cactaccttt cgttgaactt caagaacgtg aggatcgaac
    14701 gaaagactca acattctatc cgattccaac cagaagccgg tttcaatatt aaatgatggc
    14761 agagttaaat agtagttggt aaaatcagga ctcgtaaatg cattgttttc accaccggcc
    14821 atctgcaatg cctcatcata agaaggaata ttttctgagc ctccaaacat cagatgttca
    14881 aacaagtgtg caaagccggt ctgattttca ttttcatccc gggaacccac gttatagaga
    14941 atatttagaa ccgcgatttg agtcatagga tcctcgtgta ctataacacg aagaccattt
    15001 tcaagtgtaa aattttcgaa ttgtatcatt cagaagggtt ttcaaccaaa aatataataa
    15061 gccttactat ttggagaacc tatcaatgaa ttaattttgt caccattgac attaaaatga
    15121 cattcaaccg caagggactt accaaccaga ctttgattgc gctgttcacc ggcttgctca
    15181 agccgaggat gattgaagaa aagatgctta ttctgctccg gcagggaaaa atcaccaaat
    15241 ggttttccgg aattggtcag gaagcaattt ccatcggcgc tgtaaacgcg gttgagtctg
    15301 atgagtatat acttcctatg catagaaatc tcggagtctt tacaggcaga aatattcctt
    15361 taaacagact ctttgcccag ttccagggaa aattatcggg attcagcaaa ggccgggatc
    15421 gttcatttca ttttggcacc aacgagtttc atattgttgg catgatttcg cacctggggc
    15481 cacagatgag tgtggctgat ggaattgctc ttgctgataa attatcgggt gtaaaaaaag
    15541 taacccttgt atttaccggg gatggcggaa catccgaagg agactttcac gaagctatca
    15601 atgttgctgc agtttgggat ttgcccgtaa tttttattgt tgaaaataac ggttatggac
    15661 tctctacgcc aagcagcgaa caattcagat tcaaaagttt tacagaaaaa ggaccggcat
    15721 acggaattga agcctactct attaatggca acaatgtcat tgatgtttat cgtgaagtta
    15781 aaaaactcgc cacctctatt cgcgacaatc caagaccggt aattttggaa gctctcactt
    15841 tcagaatgag gggacatgaa gaggcttcag gtactaaata tgttcccaaa aaactgatgg
    15901 atcattgggc gaaaaaagat cccgttatta attaccaaaa atttttgatt gaagaaaaaa
    15961 ttcttgacaa ggaaaaaatt gcggaaatcc gggactcaat cagcaaagaa attgaatcgg
    16021 gattaaagat tgcctatgag gaagccatgc cggaaccgaa ttcagaaact gagctcgccg
    16081 atttgtacaa accgttccat gataaaatta catctccaaa taaaaaaact tcttcaaagc
    16141 gattcgttga tgctgtgtcg gatgggctga aggccggcct ggtaaaattt cctaatctaa
    16201 tctttatggg tcaggacatt gccgagtacg gcggagtgtt caaagtgtct gaaggttttg
    16261 ttgagcaatt cggcaagaaa agaattcgaa atactccgct gtgtgagtcc gctattttgg
    16321 gaatcgggtt agggctatct ataaagggat ttaagtcggt tattgaaatg caatttgcag
    16381 atttcataac atcaggattt aaccaggttg tcaataacct cgccaagtct cattatcgat
    16441 ggggtcaaaa ttctgatgtt gtaattagaa tgccgacagg cgcaggagtt gctgcaggac
    16501 cgtttcactc ccaatccaat gaagcctggt tttttcacac cccgggatta aaaattgtct
    16561 acccttctaa tccatttgat gccaaaggac tactaatcag ggctattgag gatccaaatc
    16621 cgatcctgta ttttgaacat aaagtattgt accgatctat tgccgatgaa atccccgatg
    16681 actattattc aatagaaatc gggaaaggta aactcatccg ggaaggaagt gattttagta
    16741 taattaccta tggcatgggt gttcactggg caaaaagaat tgttagtgag aaaaattact
    16801 ccgctgatat ccttgatctc agaagtctta tgccctggga taaggaagct gtagaaaaaa
    16861 ctattacaaa aaccggtaaa gttttaattc ttcatgaaga tacacttacc ggaggcattg
    16921 gtggtgaaat tgcggcctgg attggcgaaa actgttttag agctttggat gcaccaatta
    16981 tgcgcctggg tggccttgat actccaatcc cgttctcccc acctctggag ctaaacttcc
    17041 ttcctgatgg gcgattccgg caaaaaattg aggaattgct tgaattttaa taatttagag
    17101 gatattcgcg gtcaattttc atacggtttc tcaaaaatca atcgttattt actttttgtg
    17161 ataagcctta tttccatatt tttttctctt cgtggagcct tcaaatattt gaaaggcaag
    17221 gcacggctaa ttggttttgg actaatcgct attttcttcc tgacaggtac ggggaacctt
    17281 gcagcacagg atgacagacc tgccaagccc aggaaagaaa aagttaagcg atcaaaaccc
    17341 cggggtgaaa gatcagcaaa caaaaatcca cgtgtccggg aaacccgctt taaaatgcag
    17401 atgaaaaaag gagaccggcc ggaaaaccgg gatctcaccg gcagaagagt taagaggaat
    17461 caacggacaa aaggacctca gataaattat tccaattata agcctgcaag tagacgcacc
    17521 caatataaga ataaacccgg attctcctct gcaccacaaa gaatacgaac cgccagcaga
    17581 aaaggggaac acagtccgag aggggatata agtgggcaca gaattaaagt gtcacggagt
    17641 cccaaaccca gcaatcggct tacatttaat gcacccaatc ccaatgttgt caaaagaggt
    17701 aaatcaagag aaggtagaaa gacgtatcgt tccacttatg ttcgttctgt tagtggccaa
    17761 aaccgtggca aacctccttt ttcttcagcc cctaatcctt atgtggccag gagtaagaaa
    17821 tcaagagaag gtagaaccag atatgcttcc ccatccattc ggtcaagaac tgcgcaagat
    17881 gcccgttcac ggaattcaac ctcagcaggg ggtgtaaagc caccgaagag taccggagga
    17941 actattctgt caagaaggcc ttccactact gtcagaacct cccgaacaca accgggtgac
    18001 cgcgcaaaat ctttttcgag gaagcaaggt ccggttaaat caatttctgg aaacatcagg
    18061 aataaccggc cttctaatcc atttgcggtt gcaaatacaa aaggaaaaag aagtggcgat
    18121 gtagcacgaa acagagatat aacaggaaga agactgcggg aaaacaaacg atctcctggt
    18181 aagcatatca ccaaagtagc tccgaatcca tatctgaaac gcagtgaaag aggcgagagc
    18241 ggcgtttcaa ataagaaaca attggtaaaa gctggcagaa cctccaaacc gggagatcat
    18301 ttcgcaaaaa cttcgtttgg cggagtacgt acttctactc gttccggaga aactgcaacc
    18361 aatagatccg cctccggaaa aaatataaga actaagaatc tgaggtcagg cagacctgtt
    18421 tttgccggca agcaataccc cggctacatg actgcaacca aaagagggga aggaggtttc
    18481 agtggcaaat caggcaagat acgtagttct acacgacccc gggaacgggc tggagtacaa
    18541 cctggaggat ttaaaacagc tactgcccgt ggggaacgat ctaccaaccg tgatattgcc
    18601 ggaaagaggc tgagagcaag gaataaagaa agcaggacta cccaaattgt cggactgtct
    18661 caaaagagaa cccttagcag gtcgggagaa ttaatgaata atggcggcag gtcgctggag
    18721 agccggccgc ctggcaaggg tacacttcag ggaggcacat tttcaggaac tcttagagcc
    18781 ggaaagggaa ccaatgcagg gccaggtgca aaatattcag gaaatgttcg atcatttaag
    18841 cctcctaaag gtgggggtag cattacccgg cttagaaata acaaaggaaa tccattagct
    18901 gcacgcaaac ctggaaaagg gacgattgcg ggaggcagat attccggcaa catcagtaaa
    18961 ggcccggcct cgaagcctgg aaaaggggcg attcacggcg gaaatttcag tgggaacctg
    19021 agaagcagaa agggattcta caccacggag aaagcttatg gccttcgcac cgaaaaagaa
    19081 aggttgcata acaataaagg aaatgccatt gaccagaaga tggttggacg tggtactatt
    19141 cagggttccg catttcgtgg taaattgtct gccaaatcaa acaaaccggg aaaaggtgca
    19201 attgccggca acaggtattc aggcaatttg cggaaaggtc cggcaaccaa acctggcaga
    19261 gggacgatcc agggtggcaa ttaccggggg aatcttcgaa gccgaaaagg attctatacc
    19321 aatgaacgaa cctatgcagt tcgcacccaa attgatgaac ggctgcacaa taataaaggt
    19381 aatcctctgg atggaagaca agtgggtcgc ggaacaattc agggagcaac ctatcgtgga
    19441 aatctaaggg cagacaaatc acagaagcca gggaaaggtg cgattgcagg ttatcgctat
    19501 cagggaaata tcagggcaaa aaaagatctt gaaagaagtg aaggaacacg atataaagga
    19561 cggctctcag cacgcaccgc cacacccaaa attcagccac aagggcagga gtatcgcggc
    19621 aagatgagag cttctcgcgg ctatgatcac caaaaagcag gcgatgcact taagcaaaca
    19681 agagcctggt accaaatagg aacattgcga gcggataagt acgctggaaa ttaccggtat
    19741 aagtcaccaa ccagaaaaga tcaacatatt agcagtgcct ttacaggatt caacccgggg
    19801 aatgctaaga aagaaaatga tcgttttttc aaattcaaaa tctggtttgc aaaattattc
    19861 aggaatgaaa ctccacgaca tttgaaaaac aaaggaagga agccgaggta tgatcgtggt
    19921 gaaagtgata tctggtaccg ctaaaatcag aattcaaaaa ttacttcctg ctcgagcaag
    19981 tcacttaagc tgagtgcaac agggcaattt aacgcggtgt cttttagtat cttaataagg
    20041 tctgggtctg aaacaggatt tgtccacttc atccggatca cagccttagc cactttcctg
    20101 ggtgatgaag acatgaattt ctccagttcc accgacaatc cctgaatatc caaatcaaaa
    20161 ttttgagctt ttatccccat agtagtcatc atgcatgtgc caagtgaaca acataataaa
    20221 tcagtgggtg aaaatgcctc cccttttcca tgattgtcta ctggtgcatc ggtaatcaac
    20281 ttttgttcac tttttaaatg aatagattca cacctcaaat tccccaggta ttttgattcg
    20341 attatcggca tgttttttgt ctttttaggt ttcagaattg gctataattt aacaagagtg
    20401 taatttagtc attataaata gatttcggtt tgcggtgaaa aagactcttc ctttaatttt
    20461 attcctgcta ttttttgcgt tccatgcatt ttcccaaagt ggaagacagg aatttgaata
    20521 tacaagggaa tttacatggg gtatcaataa aaatacttcc ggaggtctga ttggcggctt
    20581 tgttttcaag cattccaagg cggtttcacc caggatgttt agaacaatcg gattagagat
    20641 tgtcaatatt aagcatcaaa aggaagtccg gtataactcg caattcacag gtaacttttt
    20701 catctggggc aaggaaaact atctgtattc attgcggttc caatatggaa gggatttaat
    20761 tattttcagg aaagccccac agcagggcgt gcagattatg gcaatttctg ctataggtcc
    20821 atcaattgga atcctggctc cctattatat cgaatacctt cagggaaata atgcaataag
    20881 agaacaatat gatcccgata ggcacaactt ccagaatatt ctgggtaccg gatatgtttt
    20941 ccagggtctg ggagaatcaa aattgagggc cggagccaat ctaaagcttg gaataagttt
    21001 cgaattcgga accttcaaaa caagcgtaac cggtttcgaa gttgggttcc ttgcagatgg
    21061 ctatttgggt gatttggtga ttatgcccaa ggctccggaa aataggaatc tttacccaac
    21121 cgcttacatt tcactctttt acggaacccg taaataagga ctttttgact tacatttccg
    21181 agggttaaca cttaatttta cagtatagat tatgaataat gattgaactg ccggtagttg
    21241 aaaaagacgg tgctcataag aagcgaaaac cagattggct aagggttaaa ttacctattg
    21301 gcaaagagta tgcgcatgtt cgcaaagtgg tggatgatca caaactgcac acaatttgtg
    21361 aaagtggaaa ttgtcctaac atgggtgaat gctggggcgc aggaaccgca acgttcatga
    21421 tccttggtaa tgtatgtacg cgaagttgta ctttttgtgc agtagcaacc ggccggccgc
    21481 cggagtacga tgaaggggaa cctcaaagag ttgcagaagc catcaggctt atgaaagtta
    21541 aacatgcggt cattacatct gtaaaccgtg atgaactaaa agacaggggc gctgaaatct
    21601 ggtaccagac tgttgtgaaa acaaaacaat taaatcctga aactaccatt gaaacactga
    21661 tacctgatgt aaaggcaaca tgggattcct tgtacagaat gatctccgcg ggccaggaag
    21721 ttgtatctca taatatggag acagtagaac gtctctataa aagagtaaga ccgcaggcta
    21781 aatatgaaag aagtcttgag caaattctga agacaaaaga atatggaaaa agaactaagt
    21841 ccggaattat gcttggcctt ggagaaacgg atgaagaagt atttaaggca atggatgatc
    21901 tggttgcgca tggaactgac attcttacgc tgggccaata tttgcagcca accaaaatgc
    21961 acattgaggt tgttgaattt attcaccccg ataagtttgc tttttttaaa gaagagggat
    22021 tgaaaagggg tctgaagtat gtggaatccg gacccctggt tagatcgtca tatcatgctg
    22081 agcggcatgt agatgttcct gtctaaacct tcttttctat tttaatccct ttccttagat
    22141 ttagggaatc caatccatat tcttgttatg aatagaatag taatttatat cctcctcctt
    22201 tctttttcca aactatcttt tgctcaaccc cagctttcgc tcatgccgtg gcccgaagaa
    22261 gttgtttttg gccggggaca aatgcggcta accagcgatt tcactattag cgtgtcacaa
    22321 aatgcaaccg acaaattatg cagatatgct acacgtatct taaggcgtat tgatggcagg
    22381 agcggaatgt tttttaagca ggaaactgta gccaatgacc gggtaaaccc aaacgccgct
    22441 ttattaattc aaacaaccag gcccgggctt actcaattag gagaggatga gtcttaccat
    22501 cttcgaattg aaaataatca aatatttatt tccgctgaaa cagagatcgg agcaatgcgg
    22561 gctcttgaga cggtagtaca attactgcgg gtggattcca ggggatttta ttttccggaa
    22621 ttaacaataa atgatcagcc acgattcccc tggcgtgggc ttttgatcga tgtgggaagg
    22681 catttcatgc caatcaatgt tataaaacga aaccttgatg gaatggccgc ggttaaaatg
    22741 aatgtgctgc acctccacct ttctgaagat cagggattca gaattgaaag caagatatat
    22801 cctaaacttc atgagcttgg ttcaaatggt gaatacttta ctcaggaaga gataaaagaa
    22861 ataatccggt atgcggatga tcggggcatc cgggtaatgc ctgagtttga catgcctgca
    22921 catgcttctt cgtggctggt aggttatccc gaactggcta gcgctccggg tccgtatgaa
    22981 attgaaacag gatttggcgt aaaggatccc gttatggacc ccaccagccc ggtaacctat
    23041 gtgtttctgg atcaatttat tggagagatg gctgccctgt tccctgatcc ctatttccat
    23101 attggaggag atgaaaacaa cggcgtacaa tggaatgcca gtcaatcgat tcaaaaattt
    23161 atgcgtgaga attctatcgc gaataatcat gagttgcaaa gttatttcaa tgaaaaaatt
    23221 ctttccttcc tacagaaata ccgcaaaaaa atggtggggt gggatgaaat ttatcaacct
    23281 ggaatttcca aagatatagt tattcaatcc tggcgcggta aggatgcatt gtttgaatcc
    23341 gccttcaatg gttataagac atttctttcc aatggatatt atattgacct ggcacatccc
    23401 gccattaacc attatctcaa cgacccattg ccagaagatc atcctttacc agacgataaa
    23461 gttggaaata tactgggggg tgaagctact atgtggtcag aacttattac aaaggaaacc
    23521 attgattcca gaatctggcc cagaacggcg gcgattgcgg agaggttatg gtcacctggc
    23581 tatatcaggg atgaaaggga aatgtatcgc aggttagatg acatttctat tgacctggaa
    23641 gaattgggcc tgatgcacct gagaaaccag gatgtgttgt tgagaagaat agccaatggt
    23701 cagcaaattc atgcgctcaa agaattgata ggacttcttg agccggttaa aggatacgaa
    23761 aggcacaacc gtggagtttc gctcactacc cactcccctc ttaccagact tcctgatgcg
    23821 gcggtgccgg attcaaaacc agccagggat ttcagatttt tggttgaaga ttatcttcaa
    23881 tccggggaag agaatttaag gcaggaaatt ttcaatcagt tgatgttttg gaaagaaatt
    23941 cacaaaaaac ttgaaccaat aaccgttcgt gcaccccttg cggaggatgc tttgaaatat
    24001 tccagagaca ttgaatcatt atgtgccata ggtgtacagg ccgttagcgt aaatatgctc
    24061 cattctcagc gttggaagga tgaagttagt atgctgctcg atgctgtcaa tagggagaaa
    24121 agagaatgcg aaatcatgat cgttggggct attgaagctt tagtcaaagt ggccaaataa
    24181 ttaattaatt gatcccgcga cgaattcttc cggcaatagt tgtttcgcaa ttcgcgggta
    24241 catcactttg gtttgcagga aatgccgtaa tttctgacct gcaaacatcc cttaatctgc
    24301 cggcagaatc ggttggttgg atcaccaatg cggtacagct cggttttatt gccggcactt
    24361 taatttttgc ctttttttca tttgccgata ggttctcacc ggtaagaatt tttctgcttt
    24421 gttccattat cggtggtatt tttaacctgg gcatattaat ctcagaaaat ttattttccc
    24481 tcctggcttt ccgctttctc accgggttct ttctggcagg tatttatccg gtgggaatga
    24541 aaatcgcggc aagctggtat aaagaaagtc ttggcaaagc tttgggttgg ctggtaggtg
    24601 cactggtaat tggaacttca tttccacatc tcgttaaagg tttgcatttt tctcctgatt
    24661 ggcaatgggt aatcatactt gtatcaatag ttgccatatc cgggggactg gtgatatatt
    24721 tccttgttcc tgatggcccg ttaacagcga gaaccgcctc attcagattt aatgttctgg
    24781 ctaaggcgtt tcaaagcagg gatttccgat cggcggcctt tggttacttt gggcacatgt
    24841 gggaactgta tacattttgg gcgttcactc ccgtacttat ccagtattac gcgcagcaac
    24901 atcccttgcc tgtttctact cccgtcttgt cattcataac cattggtatt ggcggggttg
    24961 gctgtatcct gggtggtctg acctctataa aaaaaggaag cgcgagagtt gcttttgcca
    25021 tgcttttgac ttcaggactt tgctgcctgt tttcagtttt tattattgaa ttcccaacac
    25081 ccctgttctt ttttttcctg ctgatttggg gattcagcgt tgtaggtgat tcacctcagt
    25141 tttcgacact ttctgccatg accgcacctc cggaatatgt gggtaccgga ttgacaatcg
    25201 tgaacagcat cggttttgct attaccataa taagtatctc gcttgttaac agcggatcgg
    25261 gctttatcga tttcaaatac ctgtttttac tcctccttcc cgggccaatt tttggacttt
    25321 ggtcgattag aaaacttgtg taacaagtcg ctaaattgat acttttatcg attttttctt
    25381 gctgtctgca taatttcccg ggacaggaat tgggcgaaat tatttaccat tcgggtgact
    25441 ttgctaatac ttcaaaatta gaatttttga taaatagcct ggcttcccaa tactaccttc
    25501 cccttgaaat tggttctagt gcatttacta tgttacaatc ttcagggcaa ttttggattg
    25561 agcgctcaag actttctttt atcaatttca aattacggat ttgattctca atggatcgga
    25621 ttttatcaag catcctgggc atttcatctg agcagtcaaa aattcccatt tcccaggctt
    25681 gtataagttc ttttgtttca tttagagtaa atccaaaatc cttcaagttt ttgattgttt
    25741 gcaaccggtc aattattctt tccgaatatt ccttgtaatt attcgggcgc ctttcacgat
    25801 gacttatttc aatcaatccc attttctcat aaaaacgtat cgtgtctttt gaaaaccctg
    25861 ttttttttga tacctcccct attaacataa aatattttct taaaatcgct tgacagtgga
    25921 ctatactcca ccgtctatat ttgcatcaaa attaaaaaat aataatcaaa tgaaacaact
    25981 atttattatt ccggttttat tactttcagt gaatctgatg gcccaaaaca aatcaacgaa
    26041 tatggaaaaa gtatccgtca aaccgcgtac tatgtcatgc aaacttacca gccctgagct
    26101 gcaagaaagg aaaattacta ttatagcaaa tcttaaagct cagattctcg aaaaaaagga
    26161 aattgataac ggatatacct ataagttctc cggaactgat gctatcattg acgaattatc
    26221 tcagttcatt aagacagaaa gaaagtgttg ctcctttttt gattttacta ttgagattga
    26281 gggtgttgaa gatggtaaca tttggcttgc cataaccggg ccggaaggcg taaaagactt
    26341 catcaaagaa gatctggatt tatgatcggg attttatgat ctggatttat aataataaag
    26401 attattgctg aaccttcctg gaaaagattc ctgggtagta attcgatatt agttaaaatc
    26461 gaattactat ctccttgatt catccaaaag tctttcatag tttttcagcg gatggactct
    26521 aaaattgatt gggaacttaa tgattcccaa aactgctgtt ggttgaccta cgctgaaacc
    26581 ccttccttaa actcttccac aggcacgcag gcacagaaaa gatttctgtc gccataggcg
    26641 ttgtcaattc tgccaaccga aggccagaat ttgctttctt tgacaaaatc cagcggaaat
    26701 actgccttag atcgtgaata ggggtaatcc cactcctcct tcaaactcat ttccaaagtg
    26761 tgcggggcgt tcttaagtac attattttcc ggatcagcat ttccctgttc aatttctctt
    26821 acctcttcac gtatggtaat taacgcatca cagaaacgat ccaattcagc ttttgattcg
    26881 ctttcggtag gttcaatcat aagggtgccg ggaacaggaa atgaaacagt tggcgcatga
    26941 aagccgtagt ccattaatcg cttggcaata tcttctactt caaccccaaa ttttttgaaa
    27001 gcccggcaat ccacaatcat ctcatgggcg caccggttat ttttccctgt atatagaatc
    27061 ggaaagtgat ccgccaatct ttctttcata tagtttgcat tcagaatagc cagctgggtc
    27121 gctttcttta acccatccct tcccatcatt gcaatatatg cataagaaat taccattata
    27181 ctcgaactcc caaagggcgc ggcagaaata gcgttgatag ctttttcacc accagtctta
    27241 atcactggat ttccgggtaa gaatggagtt aaatgctccg ctacacaaat tggtcccatg
    27301 cctggtccgc cgccgccatg aggaatacag aaagtcttat gtaaattaag atgacaaaca
    27361 tcggcaccaa tttccgccgg acttgtaagt cctacctggg cattcatgtt ggctccatcc
    27421 atataaacca gtccaccgtt gtcgtgaatt attttgcata catcctttat actttcttca
    27481 aaaactccat gggtagaagg ataagtcacc atcaatgcta ctaactgttc cctatattgc
    27541 tctgcctttg ctttcaagtc atccagatca atatttcctt tgtcatcaca tttaacgatt
    27601 accacctggg ttccggccat tactgcactg gccggatttg taccgtgtgc cgaagaagga
    27661 ataagtgtga cgttcctttt gccttcacct ttagattcat gatacgaccg aattaccatt
    27721 aacccggcat actcaccctg agctcctgag ttgggttgga gcgacactcc cggaaaacca
    27781 gtaatatcac tcaaccattc ggaaagcttt gaaaacatct ctgtgtatcc tcgtgcctga
    27841 tttatcggtg caaagggatg gatgttccca aattcaggcc aggtaacggg ttccatttct
    27901 gtcgttgcat tcaatttcat cgtacaggaa cctagtgaaa tcatggagtg agccagtgaa
    27961 agatctttat tctccaattt cttgatgtac cgcaacattt catgttcgct ataataagag
    28021 tgaaaaaccg gatgggtaag gtaatccgat tgtcttgtta atgaatccgg gaaagtaccc
    28081 tctgcaattg tatcgagcct caaggcattt ccaccggttt ctgaaaaaca gttcaaaatg
    28141 ttacttacat ctgcggtgtt ggttgtttca tcaagactta ttcctatatc tccattttca
    28201 aagtaccgga aattcatcat agcagattct gcattctttt tcacatcact ctgatttact
    28261 ccatcgggta atcctaccct cagggtatcg aaataagttt cattcttatt tacaaatcca
    28321 aggctttcca gcgattttgc caaagcctga gtaagagaat ggatcctggt ggcaatgcct
    28381 ttgagccctc gcgggccatg ataaacagcg tacattccag ccattactgc cagcaatacc
    28441 tgagccgtac agatattgga agttgccttt tctctccgga tatgttgttc ccgtgtttga
    28501 agcgccattc ggtacgcggg tttgccttgc gaatcaacag acactccgat tatccggccc
    28561 ggtatctgcc gtttgaattc atccttagtg gcaaagaaag ctgcatgcgg tccaccgtat
    28621 cccatcggaa ccccaaatcg ctgtgaagaa cccacaacaa catctgcgcc tatttccccg
    28681 ggaggcgtca aaagtgttaa tgccaacaaa tctgaagcaa ccacaaccat aattcctttg
    28741 tcatgggctg cttcaatgac ggacctgtaa tcgatagcct ttccgctgtt atttggatac
    28801 tgaagcatga ttccaaaaat tgaacccgag gttacatcca attggtccaa agattttatg
    28861 tttaacgtca cccccaaagg tttcgatctt gttttcaaaa catcgatcgt ctgcgggaat
    28921 acttcctcat caataaaaaa ttcctccgaa ttctgtcttt ctccctttct caaactttgg
    28981 agcatggcca ttgcttccgc cgccgcagtt ccttcatcaa gtaaagaagc gtttgctatt
    29041 tccattcctg ttaagtccat caccgtggtc tggaaattaa ttaacgcctc cagtctgccc
    29101 tgagctattt cagcctgata aggagtatat gcggtgtacc accccggatt ctccagaata
    29161 tttcttttta taacaccagg agtaattgta ccatagtatc ccatgcccag gtatgatttg
    29221 tatattttat tcttgattgc aagagctttc aaatgattaa ggaactcaaa ttctgtttga
    29281 gcttcaggag tattcaaatt gcgatcaaga cggattcccc gtgggaccgt ttgctcagtg
    29341 agttcatcca gagaagcagc tcccacagtt ttcagcattt ctgctacttc atcctcactg
    29401 ggtccattgt gtctgttaac aaagttatcg cttaaatgtt gattctgact catatcgaaa
    29461 ttcctccaat ttttgaagtt ttttagggct tgaaatttgg cacaaatgta ctgtaaattg
    29521 aattgaataa ttcttctttt gctcattttg aatcaattca aacaggagct aaccactaat
    29581 aaaaaatctg gttgaaataa agaatcaatg cattactttt gagccgttta acaaccttgt
    29641 aataaaccct gtatgaaaaa caaacctata ctgttgctga ttgcaatact attcagcgcg
    29701 ttgaccggat atgctcaggc ggatcagtct ttgatttata gcgaaaccat aaataatgat
    29761 gacatgtatg acaagctttc cataattgca tcagatgcat tggagggtag agaaaccggc
    29821 gaaaggggtc agaaaatggc ggcggctttt attcaatata attttgaaaa aatgggcctg
    29881 attgggccgg ttaaggattc ggaaaatcca tactttcaaa atgttccctt agtaaccagc
    29941 aagccgggag atgtatggct tcgggtaaaa gggaaagatc ttaagaattt tgaagatatt
    30001 ctttatttag gaaatgccag ccttgattat aaagataagg aagttattct ggcaggtaac
    30061 ggacaagatg ctgattatga agcagtcgat gtaaatggaa agagcgttat catttactta
    30121 ggtgaaggca ccaggcgtgg tgtttcgaac aaagcttatg aaaatggagc tgaatttgtg
    30181 ctattggtcc cggctggcaa tgatgatgac tttcgaaatt acattggaag gatgtcacgt
    30241 tttgccaggg gaggcggatt atcccttgac ttgcccaacc gggaggcatc aaaaggtgcg
    30301 gtttttattt cttctgcaat cgcatcggat attcttggta cttccgatga taagattcaa
    30361 aaagcgctgg cttctgaaga caaaaatcaa ttaaaaaaaa ttccaacagg taaattcact
    30421 tttaattcag aaatggtcac tgagaaaata acatctgaaa atgttttagg atttcttgaa
    30481 ggaactgata aaaaagagga gttaataatt attacggcac attatgatca cataggaaga
    30541 aatggggatc aaattaataa cggagcggat gatgatggtt cgggaactac ctcggtaatt
    30601 gaaattgcag aagcatttgt ggcagcgaag aaaaatggta atgggccgcg ccgctccatc
    30661 ttgtttatga cggtaaccgg tgaagaaaag ggattgctgg gatcacagta ttataccgct
    30721 aacccggtat ttccgcttaa aaatacagtg gttaacctta atattgacat ggttggaagg
    30781 gttgatccca agcacgtaga aaatccagat tatgtttatc tggttggatc tgaccgacta
    30841 tcctccgaac tgcatgaaat aagcgaaaag gtaaatgaaa cctttagcca attggatctc
    30901 gattatacgt ataacgatga gaatcatcct gagagaatct attatcgctc cgatcactgg
    30961 aattttgcca aaaacaacgt ccctattatt ttctacttta atggtacaca tgaagattat
    31021 catcggccta ccgatactat tgataagata aatttcgata tgctggttaa gcgggctcga
    31081 ttggtatatc atacagcatg ggtactttcc aacagggatg aaaggttggt tgtggacaag
    31141 gtccaggaaa ctactattga caccaggtag caatcgacgg aactcatttt cttttccttt
    31201 ttaattttag atgaaatgct cctctaaagg tggggtcttc actctttttc tgcttatcaa
    31261 tttcccggtt cattacttgc tgttcctcaa atggagtcat ctctactttt atatcagatg
    31321 gcaaattcaa taatggaatc tttgccttga taagtttctc aatttttctg atatgatatt
    31381 catcggccgg acttacaaac gtaatggcat cgccttttgc agaagctctc cctgtccttc
    31441 cgattcggtg aacataatct tcatattgaa cgggaacatc aaaattgatc acatgactta
    31501 tttcagctac atcaattcca cgcgcagcaa catctgttgc gactaaaact ctgagttcac
    31561 cattactaaa ttgattaatt gcattcaatc tgaaattctg atctttatta gaatgaataa
    31621 cttttacctc tccaattttt tttcgcagca aaaatttata aatgttattg gccgattgct
    31681 tggtcttcac aaaaatcaac agccgggagt aattttcatt ctccaataaa ttcagaagaa
    31741 gattaagttt tgttttgaaa tttggaacac tgagtttttt ctgaacaaca ttttccgccg
    31801 gagtagattc gggtgtcact tcagctttga atggaaaaac cagaaattca ttgcaaaaag
    31861 catttacttt ttccggcatg gtagccgaga ataacagatt ttgtctcttt gccggtacaa
    31921 cttcgaaaat ccgcctgagc tgaggagcaa aacccatatc catcattctg tccgcttcgt
    31981 ccaaaaccat tgttttcaat tcttttgtaa tcagctcacc ggacttgtaa acatcccaaa
    32041 atcttccagg cgttgcaatc aggaggtcaa ttcctgtttt taccaaatca atctgctgcc
    32101 tgattccttt tcctccgaat atccccgcca tcctcaaatc ggtataggcc gacaatgtcc
    32161 gcgcattttc ttcaatctgg ataaccaact ccctggtagg cgctagtatc agggctcttg
    32221 gcgatttacc ttgagcataa tttaactttt ttaataacgg taacagataa gctgcagttt
    32281 tcccggtacc cgtctgtgca attccaatta catcatgccc tgcctggata agtggaatgg
    32341 cttttgtctg aatggccgta ggtaagttgt atccggcttc cgcaattgcc gttaataatt
    32401 gcttgttgag tttgaaattg ctgaatccca ctacttagct caaattggaa tagcgctaaa
    32461 atagataatt acattcaatc gcttatcttt aaattaaaaa gaaacaggtg gtatccgata
    32521 ttctcatact caatggaaaa attgttaatg atggaactat taccgaaaaa gatatcctgg
    32581 taaggaatga gaaaattgaa caaattgctg gtgatcttag ttctattcca gccaaaaaaa
    32641 ttataaatgc cgccggaaaa attattttgc cgggagttat tgatgatcag gttcattttc
    32701 gagaaccggg attaacacat aaggccagta tacttactga agctaaagct gctgtggcgg
    32761 gtggtgtaac tacctatatg gaaatgccta acactattcc caacacatta acacaggagt
    32821 tgcttgaaca aaaatatcag attgcaagtc agaaaagcct tgctaactat agcttctttt
    32881 tgggagcttc taatgataac cttgaggaag ttaagaaaac agatcctaat agcgtatgtg
    32941 gaattaaagt ttttatgggt tcatcgaccg gcaatatgct tgtcgacaat gaaaaaactc
    33001 ttgaagctat ctttcgggaa gcacaggttt tggttgctac ccattgtgaa gatgaaggaa
    33061 ccatcagaaa aaacatggca gcatttactg agaagtatgg agaagagatt cctattgaag
    33121 cgcatcctct aatcagaagt catgaagctt gcttcaaaag ttcttcatta gctgtcgagc
    33181 tggccaaaaa atacgatacg aggttgcata ttcttcatat ttctactgca aaggaattgg
    33241 agctatttaa acgcggtcct gtggaaaaca aaagaattac ttctgaggct tgtattcacc
    33301 atatgtggtt tgatgaccag gattacgcaa ccaagggaac cttaataaag tggaatccgg
    33361 cagtgaaaac tgaagcagac aaaattgcta ttttccaggg gttactggat ggtgttattg
    33421 atatagtcgc gacggatcac gcgccacaca ctttggaaga aaaacaaaat aaatatctta
    33481 atgccccatc aggcggcccg cttgtgcagc atagcctggt tgcgatgctt gaattttatc
    33541 atcgaaataa aatttccctt gaacaaattg tcgagaaaat gtgtcataat cctgcaaagg
    33601 cttttcaaat tgaagaccgg ggatttataa aagaaggtta ttttgctgac ctggtggtag
    33661 ttgatttgga taatccctgg acggttagta aagaaaacat cctggcgcat tgcggctggt
    33721 caccctttga aggcactgaa ttcaaatcaa atatctccac caccctggta agcgggcatc
    33781 ctgcatttga aaacggaatg tttaatcaaa gtaaaacagg aagacgaatt acctttgatc
    33841 gctaatcaaa ctctccgtac tttatttgag aattgaacat gtctgcttac atttgcagtc
    33901 ctaaaacggt ggatgtagct cagcaggtta gagcactaga ttgtggttct aggggtcgcc
    33961 ggttcgatcc cggtcttcca cccaaagaga agcttccgtt accggaggct ttttttgttt
    34021 catctctttc ccaatgaact ggagtaaaca tcatataata acatcagcgt gtgccacact
    34081 tccacttttt attttaggtg aaaattgatt gtttttatgt cgataagtgt ggctcacgca
    34141 aagcttccat tttaaattaa taccataaga ttaaatttaa attggtctga aatttggatg
    34201 ctttttattg ttaaacattt tttgtaattt ggaattaaag cgagattgaa agtggaggaa
    34261 tatcaaacat ttgaaaaact ttcattacat gataagataa aaactctgta tgtaaaggga
    34321 acttttatca catccatccg gtattatgag tacaaaataa atttatatct gttcaacgga
    34381 atttacctgg aggttttcta tcatcataag aaagatcaga ttgaaaaaat agaacagctt
    34441 cagcgttcaa atacccggat gaaattctat ctggaccagg taaaactgtg cggattgccc
    34501 atttaaatcc ttttcattca cccatcactt attttttcct tttaacattt tttaggaaac
    34561 aattttccct caatttccgt catagtataa ggttaggtaa gtaaaaaggt cagaaaaatt
    34621 atcatagttt aggattaggt aatcatatat taagactttt tactgggttg ttttattagg
    34681 ccagtttgtt ctgcaaactg gccttccttt ttattgtctc ataaatatcc agcacttgag
    34741 gtattaatgc tgaagtattg tcattcacaa ttactgcatc ggccaacagg ctggatttct
    34801 cttgcggcca ctgattgctt attatttccc tgatttggga ttctgttctg ttatctcttt
    34861 ttaagacacg ttcaattcga attatatcgg gtgcagtaac cactataaga aagttcaatc
    34921 gctttgcaat atctttttca tacaacaaag cggcttcatt tataacaata tttgatgttt
    34981 gttcagtcag ccatttacta taatcccggc caactttagg atgaaccatt tggtttaacc
    35041 tctccagttc tgcggggtta ttaaaaactt tggcagcaat gaatttcctg tcaagaccgt
    35101 gaactccata agattcatct ccaaaagctc gtataatttc agattttagt tccgggtctt
    35161 cactcattaa ttgttttgcc ctggaatcag aatcatagac aggaattccc ataactgtga
    35221 atatctttgt tactacagtt tttccagaac ctattccacc ggtaacacct acctgaaaat
    35281 gtttaatcat gagatacttt aacccggcca gattctatgt taatatcaag gacatcgcct
    35341 gggtattttc tgattaaggg ttgaattgtt gaatctgccc gggccatgcg ggaaagatct
    35401 aaaatgattt cgaaggctga tgaatcagtt gcagctcgat cttctctaac cataaaagag
    35461 attgtaacat ttggatttct tacgtaccat gaactgtcgg caggaaagtt tattaaagtt
    35521 gattttacag ttttagaaac ccggagaaat cgacgaacct caaatgatac tcccacattt
    35581 ggtggattta ccgaaaacag gtggtctgct cccgttttaa taattaccat ttgcaagtaa
    35641 tctccatcaa tggcctgctc cggaatctct acaagaaact cattattcaa caaagtcatc
    35701 attgattcag gaccagtaaa tattgctgaa tcaacagata acgatattga actaatcagg
    35761 taatggtttt catccaatga aatggacgcg ctatcgatct taagtttcaa tttcctgtca
    35821 attttgtttt caatatttaa tgccagtgaa tcggtaacaa tgaagttaag ttgaaattca
    35881 tcaagctgat cgctaatgct gctcctaaaa gaatttcctg ccaccctcct ttgcaaaggg
    35941 tcttccaaaa ccaggataat aggttcgata ttaaaccaat ttgttctcct gattaagttc
    36001 caaccaccac cggttaagtt aatttggacc cggcttggaa gctgggaaac tgctatgtat
    36061 ttcgattcgt caaatatcag gtttatcggg taacttatag tggtggtgta tgatttgttt
    36121 aaggagttga aaaaccaaaa tgtggttgct cctattatac acagtattat tactttccaa
    36181 ttggcaaccc gatcgggaat tatgatgttt ttaattccat gccatatgtt cttaaccatt
    36241 tccaacaaaa tatcaattag ggggtttgtc gctttttgct ggcttccatg ctgatgctgg
    36301 atttttcaaa tgtgagcttc agacccttat caacttcaag cacaatggta tcattctcca
    36361 cattgagtac tttaccatgc aatccgccga ttgttattac taaatctccc ttttttactt
    36421 cctccaaaaa ttttttttgc tgcttttgtt tcttttgctg cggtcttatc atgaaaaaat
    36481 aaaaaataag ggcaattgct gcaaagaaaa ttattgatga atagccactt ccctggccat
    36541 ttgcctgtgc aagaatggga tgtatcattt tatattattt taaggattgc actaaaatac
    36601 gatttgcact aaactaaaag aacttgtaat ccgaaaatta caagttcttt tttctaaaca
    36661 agatttgtcc ttaattcctt accggaccgt cagctgcatc ggcagatgtt ttaggattaa
    36721 caaaagtctt aatccttagt ctgctaatct ttggataggt attagccgtg atggtaacgg
    36781 tcttgttttg cacgttaggc tttcctttac tatcaaattt tacctgaact tcgcccgttg
    36841 cgcctggagc aacaggttct tctgtccatg ctggcacagt acaaccacaa gatgcctggg
    36901 ctttttcaat aattagagga gcttcaccgg tattcttgaa aagaaaggta tgttgcacaa
    36961 catccccgtc ggtaatatta ccgaaatcat gttcttcgcc gccaatccat tcaaaagcag
    37021 gaagcgggcc atcaggtttt atattagaat ccgtggtttc cgcagcagcc tcagtagtgc
    37081 tggcgggcgt tgcggtgccg gtagttgccg taccgccatt ttcaacttcc gcaagcttat
    37141 cctgcaattc ggcaattctc ttttcagagg tcttgctgtc acagcctgcg aaaatcacag
    37201 ctgctaaaaa taaggttgcg cttaataatc ttgtcatctt cattttgcta ataaaatttc
    37261 agttaaacat catttattct catttcggat atgaccaatc aaattgtcaa catcctgcaa
    37321 taatttctct gccttttcct tggcgtcatt gataactttt tcaccttctg atttcgcttt
    37381 cgattttggt aaatcatctt taccactaat taaatcttca agcatatcct caagagtttt
    37441 cttatactta tccaaccggt aagtaagttt atcacgggta tttgatcctt tatccggtgc
    37501 ataaagtatt cctaatattg caccggttgc agcacccagt aaaaaggcca gaaatgcatt
    37561 cgatttattg ctcatgtttc ttttacttat tgtccaacaa acccctgccg ctttttttga
    37621 ttagtccctg agatttcaat tcctgagtta ttccatccaa aaccccattg ataaactcct
    37681 tgcttttagg ggtgctatat ctcttcgaaa cttcaatata ttcatttatc gtcactttga
    37741 cggggatgtt tgggaaattt tttaattctg ccaccgctaa actaagtatg attttgtcta
    37801 ttaatgcaag tctttccagt tcccaatttt taacctttcg tcctatcaac gcttcgatat
    37861 ttctttcttc ttctactacc cgatcaaaca aaacttcaaa gaactcttta tcatcttccc
    37921 aatccattga tattgaaaat cgggggaact ccttttcttc ctcgatttcc tttagggatt
    37981 tctttaccat ccctttaact actgcctcat gttctgacca tcttagatct tcttcttcaa
    38041 aaaatgcacc aattacctgg tttcttaaga taacatcacg aagcaaatga tcaatcagtt
    38101 ttttatctgc aataaaatcg gttttatcaa gttttagata ctcgtcaaac tgactgtctt
    38161 ttcttataat atcccgatac cattccctaa ctttttcttt atgggaagtc catgatattc
    38221 ctgatgctaa tatggcactc tggactgaat tatcatattc aatcttcctg attaacaaat
    38281 tcttaacaaa attctccggt ggagttccaa aatttgactt aatagtggag gcaacgtttt
    38341 tgaattcaac caggagttga agtatccgaa ggtaggttgt gcgggttcct tcgatatcca
    38401 gaagcattct ttttttgagg aatttgaaat cctttttcag gtcattttca tacttgatga
    38461 acgcttcatt aacaagtttc tcaatttcct tgactttgtg gctgcatttt ggatttactt
    38521 cttcaatttt atcatcaaag atttttagtg cctccctttg tctgacagcc agcattcgaa
    38581 tgtcctcatt gttcatggtg tccatgtccg gtccaaggtt tgcagccaat gattcgcgag
    38641 ccatttgctt aagggaattc tttccctgta agtaagcaaa ggtggtttgc aagacctcta
    38701 tacgcaggat tctcctgttg agcatttcaa ataatgttaa agaacgaagt tttatttagt
    38761 tacagttttt ttcttaaaaa gtgagtttaa tatttcctat tgacttaatg cgtttattgg
    38821 ccatttccat ggctgcttct tgtggagaaa cattattatc atctgagaat accgcaatgt
    38881 tgatgcaagt gtcatagata taatcggcca tctggtaagc tctttcgtaa ttatgattgc
    38941 ccaggtattc caaataaaca ttaatcaagc cacctgagtt aatcagaaaa tcgggtgtat
    39001 acgctatttt ttgatccatc agcatgtatc catgtttgat ctcatcagcc agctggttat
    39061 tagctgatcc tgctattatg gcgcatttta gtttaggtat tgtttcatca ttgagagtgg
    39121 ctcctaatgc gcagggagaa tagatatctg catcagtttc gtacactaaa tcatgttcaa
    39181 taaccttcac ctttcctttt gtcgcaattg cttttgcttt ttcctcaaag atatccgaca
    39241 gataaacaat tgcattttct ttcagtaagt gttccactaa atatccccca acctgtccgg
    39301 caccctgtac catcactttc tttccttcaa ggctgtcaga tccaaatact ttctttgcca
    39361 ttgccttcat acccacataa gtgccgtaag cagttaccgg cgatggatca cctgctccac
    39421 ccattgattt tggcagcccg gtaacatatt ttgtttccat atggatatat tccatgtcac
    39481 gggttttcat atttacgtct tccgcagtgt agtatttacc tccaaggctg ttgataaact
    39541 tgccaaatct tcgcatcaaa gcttcggttt tcatcgttct tgcatcacca atgatgacag
    39601 cttttccacc acccaggtta agtccgctga ttgcggcttt gaacgtcatg gcgcgggaaa
    39661 gtctaagtac atcattcaac gcttctgact cagagttgta ggcccacatc ctggttccgc
    39721 ctaaggatgg accaagcaca gtattgtgaa tggctatgat tgctttaaga cctgttcccg
    39781 ggtcgttgca gaagacaacc tgctcgtgac ccagccgggt cacttcttca aacaaggagg
    39841 aatttgactt ttcaaccgct tcctttattt ccatcgtccg tgctttaatt ttttattgta
    39901 tttttgttcc tatgccgtgt ctcaggtcta aaaaaggtaa cgcaaaagta gtcttttttg
    39961 ttaagttaaa aaataccgat ttgagtttga aaagtacgtg ggaagcgggt atttataaga
    40021 aatgaaggaa ttatcatacc tcaataaata tcttctaaaa tataagtatc atttcatact
    40081 cgggataatc ttcaccatta tcaccaatat ttttcagata ataccagccc agatggtgag
    40141 acattctctt gacctgataa ttgccaatat tgatttatac cggaattatg ccggattgaa
    40201 tctgcagaat tcatactatg atatttttgc caggtacatt ttgatttatg gcgggattat
    40261 tttactcatg gcacttttgc ggggtttctt ccttttcctg gtcaggcaaa ctctgattgt
    40321 gatgtccaga ctaattgagt acgacctgaa aaatgaaatt taccggcatt atcaggaact
    40381 tccaacctcc ttttacagga gaaacagcac cggagatttg atgaacagaa tatctgaaga
    40441 tgtaagcaaa gtgagaatgt acctgggacc atcaatcatg tatggtatta acctgattac
    40501 gctcttcgcc attcttattc catacatgtt aacaatcagt gtaaaactaa ctctgtttgc
    40561 tttgctgccg ttacctattc tttctttcag catttatttt gtaaacaaca ttataaacag
    40621 aaggtcagag gcaatacaaa agcagcaatc caaattatca acgtttgtgc aggaagcctt
    40681 ttcaggaatt cgtgttttga aatcttttgt cagagaagaa gattcatcca gaaaatttga
    40741 ggaagagaat acaaagtaca agaacaaatc cctgagactc acaatggtta actcattgtt
    40801 tttcccgatt gtaatggcgc taattggctt gagtgtaatc ctcacagtat ttgttggcgg
    40861 aaatgaggtc ataagcgggg cattgacttt tggaaatatt gcggaattca ttttgtatgt
    40921 taattacctt acctggcctg taactgccct gggttggatt tccagtatta cccagcgagc
    40981 tgctgcttct caaagaagaa tcaatgaatt tatgctagtc aaaaatgata ttacaagtga
    41041 aaagaatatt cagcaggaaa taaggggaga tattgagttc aacggagtat cattcaccta
    41101 tccggattcc ggtattgttg cgctcagtag tcttgacttc aaactcaaca gcggggaatc
    41161 tctggccatt attggaacta ccggatcggg taagagtacg gttgctaatt tgttatgccg
    41221 tttatatgat cccactgagg gtaaaatcac tattgatgaa attaatctaa aagattatca
    41281 tctcgggtcc atcaggagcc aaattggtta tgttccgcag gatgtgttcc tctttagtga
    41341 caccatcttt aataatattg cattcggctc tatggagatt cctcctgaga aagtaatgca
    41401 ggctgccagg gatgctgatc tttatggtaa tgtgatggat ttccccgacc agttcaatac
    41461 ccgcgtgggt gagcgcggaa tcacactctc cggcggacaa aagcaacgag ttagtattgc
    41521 ccgtgccatc gttagagatc caaaaattct tattttggat gacagtttga gtgcagttga
    41581 tactaagacg gaaaatacta ttctgaaaag catgaaactt ctcatgaagg atcgcaccac
    41641 cattattatt tctcaccggg tatcaagtgc taaactggcg gataaaataa ttgtactgga
    41701 tgatggggta attgttgagc aaggcaccaa tgaatcgctt ttgaagagtg acggtgtgta
    41761 caggagtctt tacgagaagc agttgcttgc ggaagagctg cttgaagact agctatctat
    41821 ccagtagcgt atttttatac tttacagttt catcccggga ttgattccac agctccattc
    41881 cgccaagcat attatttact tttgtaaagc cgttttctgc caaatattcg gaagctcttg
    41941 cactgcgaat tcccaccagg cagtagacta ttatttcctt attcttatat tctgccagtt
    42001 ctttgattcg agaacccaat tcttctacat gaatgtgttt tgcatctttt atcctgccca
    42061 aaggagatcg attaaattca ccctccgtac gaacgtccag gaacaggaca ttagttactc
    42121 tcgactgcaa ttcgcacact tcttcaggat caagatttgt gtaattaaga ccttcgcttt
    42181 tattgtcctt gttcagtaat gtcattgagc aggccggaca cttgcctggc tccttgaata
    42241 cctttccatc atttgaacat ccgcatggag gacaaacgta tcccgccttt tgggtaaacg
    42301 cgtgcaatga aaaaactcca attaagatga acattattac aattctcata taattaaagg
    42361 ttgggttttg atttttctat cttattcatt ctttatcgtg tttaccgggt ttatggatgc
    42421 ggcactccat gaacggtgaa ttacgctaat tgctacaaca atcattgcaa taactgttgc
    42481 cataataaaa tttaaataat tgatttcaac acggtatgta aaattttcaa gccatgcatc
    42541 cattacaaat aaagcaaatg gagtggaaaa aaataaccca ataaggacga ttcgccgaaa
    42601 atcatacaaa atcaggagga taattatgta cgtaaagaaa atcataagtg gttcagaaga
    42661 tgtacctgat tccagctacg gcctgaaatc ggggtccaag gccatttgcc tccagtatca
    42721 ggaacatatt cccctcaaaa aatatggaaa cgggtatggc gaaattcgca aattccaatc
    42781 ccaggaaaat atcagggccg taaaagaatt gttcccttct ggtctcctgc tccatggtaa
    42841 acccatttgt atcggggtca ttatagcgat agagaatctt ggtgtttttg aattgatagc
    42901 ctcctccaac ataccagtcc agaccttcaa gagatttgat ttcactatgg atcataattc
    42961 tgaattgtcc aactaaatca ttctttaccg agtgagataa gtaattcagg gtatccagaa
    43021 attcgaagtt tgagaagtta tcctggtaga attttttata aacaaaactt gctccatgcc
    43081 caacagctat ttccattgcc aggaatttgc tgacataaag tttgtatgta actgcgaatg
    43141 gatcgccaac ttttattcct attccctggt atgggtattc attaggtata aataccggac
    43201 atataatttt cattttcctg ttatgaggca tttttaccgc actccttttg cgaacagacc
    43261 tctgtgcgtc tgcttcgttg ccaagcaaaa tcaggaaaaa aggaaagata aaaaatagaa
    43321 ttattttgta ccctcttttg cgcaaaattg acaaaattta tttctacatg aatataaaga
    43381 agctttaatt agtaacctat taacctgcct ctttatattg catcttgtgt aactgtgaat
    43441 acaaaccact tttggtcaac aattcttcat gcgagccttc ttctttaatt tcaccctttt
    43501 caaaaactaa aatcctggtt gcattttgaa ttgttgaaag tctatgtgca ataacaatag
    43561 aagttctgcc ctccatcatt tttgaaatgg cctcctgaat catctcttcg gtttcggtat
    43621 ccactgaaga ggttgcttca tcaagaacaa gaatttttgg atcataaacc attgccctta
    43681 taaatgaaat tagctgacgt tgacctaccg aaagggttga gcctcgctcc atcacattgt
    43741 aatccaattg tccaggcaag cgttcaataa attttttagc acccacggag tcggccgctt
    43801 tccaaatatc ttcatcagta atttcactgt ttcccagggt tatgttttga cgaattgtat
    43861 cagagaaaag aaaaacatcc tgaaggacaa ctccaatatt cttccttaaa ttatccaaag
    43921 ggaaatctct gatattgatg tcatccacgg taatagtacc cctgttgatt tcataaaacc
    43981 tgtttaggag gttgataaca gaagttttgc cggctcctgt cgcgccaacc agggcaattg
    44041 tttcacctct gttcactttg aaggaaatat ctttcaatac ataatcagtg gtgttgtagg
    44101 caaaccaaac atttttgaaa tttaccgccc catgaatttt atcaacctgg gtagtccctt
    44161 catcgggaat ataatcttta ctatccaata gtttcatgat tctggatgtg cttactatcc
    44221 cgagctgtaa agtattgaac cggtcggcta tcattcggat tggcctgaag aacatattaa
    44281 tatacattat aaaagctaca agcattccga gagtgattcc tgtttgctca aaattgatta
    44341 cacctttagc tccataccaa actaataaac ctattcccgc cgcaccgatt acttctgcca
    44401 caggaaaata aatggagtaa tacattactg aacggatatt ggctcgctta tgatcccgat
    44461 taatattttt aaacttttgc aattcccgct cctcactgtt aaatatctga acaatactca
    44521 ttccggtgac gtgttcttga acaaaagagt taagatttgc aaccgcattt cttacctggt
    44581 tgaaagcaac tttaattttt tccttaaaga tgtaagtact aataatgagg ataggaaagc
    44641 ttgccagact gacaagagcc agtctccagt cagtataaaa cattaatccc aagatgaaaa
    44701 caagttgcaa taaatctcca atcatggctg ccaatccttc actaaataca tccagcaaag
    44761 tttcaacatc agatatagtc ctggttacca gcctgccaat tggggtgtta tcaaagaatt
    44821 taagcttaag ggaaagcaag tgtttatata actgaatgcg gatatccctg attatgaatt
    44881 gtcctaacca gccggacaag taagtgtgga tatactgaac tatggcattg acgacgagga
    44941 gtcctactaa tataagcatc ataaacctga gcccggcata gtctcctttc ataacatcgt
    45001 tatccagagt caactgaacg atgtaaggcc gcaaaggggc aagtattgcc agggttaagg
    45061 tcaggaaaat gagtaggtaa aacttaaaac gatatggccc gacgaatttg aggagccttt
    45121 tcagcacctg gaaatcaata actcggccgc taatattttc ctgttccaaa aaaatgaatg
    45181 tttaaacacc caaagttaag aacttttcac tttaaatata cctggaggat atttaacctt
    45241 tgtcaggcat aaaccttttg ccggtgcggc cggccctgct aatttccgat ccttgctatt
    45301 taggatttta gcaaattccc tgctggttat agacccatca ccaaccatta tcaaagttcc
    45361 cactattgat cgtaccattc cccgtagaaa cctgtttgct gtaattaaga aaataaggct
    45421 atcgccattt tctttccaaa tagcccggct gacatcacaa tcgtgtccgg gaacagccat
    45481 gctattacgg caaaagcttt taaaactctt tttgcctatc aagtaagttg ctgccagatt
    45541 cattgcgtca acatccaggg gcttcctgat ctctactgct gtccaggaaa gaaatggatc
    45601 ttttcttttt attatgtgat attcataact tcttgaagta gcatcaaacc ggcagctagc
    45661 atttggttta actggtcgaa tacttttaac tgagatgtcc ccgggcagta aagaattcat
    45721 tttagaaatt agtatttccg gttctataat tttggtggta tccagatgaa agaattgttg
    45781 tgaagcatgc actcccgtgt ccgttctgct gcttccaagt gtttcaaccg gttcacgcaa
    45841 aagaatactc agagattcat tgaaaacctg ctgaacagca agcgcatttt tctgaatttg
    45901 ccatccatgg tagttagttc ccttatagga gatttcaaaa aagtaccgca tgcttcgtca
    45961 gcatttttca taaattaccg cagctccctg gccaacgccg acacacatgg ttgccaatcc
    46021 atattttgct ttggttcttt tttgcatttc gtgaataagg gttccgctta ttctggcacc
    46081 actgcatcca agggggtgtc cgattgcgat agcaccacca ttaacattta caatttcggg
    46141 attcaattct aaatcccgga tgcaggcaag cgactggctg gcaaatgctt catttaattc
    46201 aatcaaatcc aaatcattta ttttcaatcc cgctcttttc aaagctttcc tcgtcgccgg
    46261 taccggacca attcccatgg tatccggatt taccccggcg atagccactg atacaatccg
    46321 ggccatcggc ttaaggtcca attctttcag agtttcttca cttacaacca ggcatgccgc
    46381 tgctccgtcg tttattcctg aagaattccc tgccgtaaca gttccatctt ttttgaaaac
    46441 aggtcttaat cctgccagaa cctgcataga ggagagccgc ggctgctcgt cttttgcgaa
    46501 aattacgacc tcctctttct ttcttagtat ttctacgcta acaatttcgg catcaaattt
    46561 tgaaccttga tgagcaaggc tatacttgag ttgagagcta tgtgcaaact catcttgctc
    46621 agtacgggaa atcttccatt ggctcgcgac attctcggca gtttcaccca tactaaaagg
    46681 ataaaacaga tcaaccagtt tcggattagc gaatcgccag ccgatgctgg tgtcaaaaat
    46741 ttcaacattc cttgaatacg ctgattccgc tttgctcatt acaaaggggg ctcgcgacat
    46801 gctttccgaa cctccggcaa tataaatttt accttctcca agctgaacag ccctcgaagc
    46861 atccataatg gcttgcagcc ctgatgcgca cagacgatta acagtgactc caccggtttc
    46921 aattttcaac cctgccaaaa gcaatgccat ccgggcaaca tttcggttgt cttcgccggc
    46981 ttggttcgcc gctccaaaaa ttacatcttc gatcaaagat ggatcaacgt tttgatttct
    47041 ttctaacagc ttaataataa catgagcggc caggtcatca ggtctcactg acgcaagcga
    47101 tcctgcaaat ttcccgattg gagttctgat aatatctact atatatgctg atttcattga
    47161 gttatgcgca ggattggaaa ccaaaaggta atacctttat tttgtatcgc aatttaaccc
    47221 aacgcataca tgttacaaag gatacaaact gtatttctat tcctggtatc tctgcttatg
    47281 gtagttgatg ttttttcacc catttggata aatattaata ttgaaaccgg ggaaagccac
    47341 attctctata gcgcatttta tgaattcatt cctgcaacag gtgagcccgc acaaaatact
    47401 tttttccctt atacaattgt tagcctcact gccctgatag ctgccggaat tgcattctac
    47461 gagattttcc agtacaagga ccgactaacc caaatgaagc tgggagctct taactctata
    47521 attatgacag tttgcctggg ctgttcagtt tggtttgcta cagacggcca acagacctgg
    47581 atgccaagaa acaccggcag ctatggctat ggattatttc taccagctgt cgccatgatt
    47641 ctgaatataa ttgccaacag attcatcaga aaagatgaaa aactggtcag atcggtggat
    47701 agaatccgct aagactgtca tagtgtcact tatgagggtg aattgataga atggaatgga
    47761 ttttgaaaaa gcgacactag ttaatcacga actatataag caatggctga aaaaggcaca
    47821 atatcaataa atacggaaaa tatttttccg ataataaaga agtttcttta ttccgatcat
    47881 gaaatcttcc tgagagaatt ggtttcaaat gctgtagatg caactcaaaa gcttaaacgt
    47941 ttgtcggccc tgggcgaact taaagaagac attggggacc aggatctgac tattgaagtg
    48001 aagttcgata agaagaaaaa gactatcacg gtgagtgaca tgggaattgg aatgacgttg
    48061 gatgagatta agaaatacat taatgacatt gcgttttccg gtgctacaga tttcctggaa
    48121 aaatataaag acaaagaagc cgcacagcaa ttcatcggac attttgggct tggtttttat
    48181 tcttccttta tggtctctaa gaaggttgaa atttttacca gaagttatca gcctggtgca
    48241 aaacctgctt actgggaatg tgatggaaca actgaatttt caatttctga ttcagaaaaa
    48301 aaagatagag gaactgacat cgtcctccac atcgcgaaag attcggaaga gtttttagat
    48361 gagttcagat tgaaaggaat cctggaaaaa tattgcaggt tcctgccggt taaaataaaa
    48421 ttcggtacaa aagaagagaa agtacctgat ggtaaagaca aagacggtaa agaaaaattt
    48481 aaaaatgtta ctgttgatac cattataaat aatcctgtac ctctttggac cagaacccct
    48541 gcagatctga aggacgaaga ttatctgaaa ttttataagg acttgtaccc ctttcaggag
    48601 gaaccattat tctggattca tctaaatgtt gattatcctt ttaacctgac gggaatttta
    48661 tattttccga aaatcaaaaa tgattttgag ggtcacaaga ataaaattca actttattca
    48721 cgccaggtat ttattactga tgaagtgaag gatgttgtcc cggaattcct aatgttgttg
    48781 cacggagtta ttgattctcc cgatattccg ttgaatgttt cccgtagttt cctgcaatcg
    48841 gacagcaatg taaaaaagat caatgcccac attaccaaaa aagttgccga taagctttcc
    48901 gaacttttta agaaagacag ggaaagtttt gaaaagaaat gggatgacat cgccatcttt
    48961 gtgaaatatg ggatgattag cgaggagaag ttctacgata aggctaaaga cttttgctta
    49021 ttaaagaata ccgaatcaaa atactccacc attgaagagt ataagaaaaa agtaaaagat
    49081 aatcagacgg ataaaaacaa agaattaatc tttttgtata caacaaatcc tgaaaaacaa
    49141 gacacttata tatcagctgc gcgaaagcgg tcctatgata tactgatgat gaacggagct
    49201 atcgactcac atttcatcaa taatcttgaa cagaaattag aaaagaccca tctccgaagg
    49261 gtggattcat ccacaacaga taaattggtt gataaagatg aaaaggtgga aagcgtgctt
    49321 tctgaagagg atactaaaaa agtgaaagaa atatttgaaa aggccataaa caatccatcc
    49381 atgattcttt cggttgaatc gcatgcgccc gatgaaatgc ctgttattat aactcaatct
    49441 gaattcatgc ggagaatgag tgatatggca gcctcggggg gaatgggttt tatgggcaac
    49501 atgccaaagg aactcaacat cgtagttaat gcaaatcata gcatcatcag taaaattctg
    49561 aaatcaagaa aagaggaaaa gaaaacggaa ttagcaaaac aggcttatga tttggctctg
    49621 ctttctcaga atatgctgga tggtgccgat ctgaccaatt tcattcaaag aagcgtgcaa
    49681 ctggcatccg actaactgtt tacttaattg aagtaataat tggttttaca aggcgttaaa
    49741 taattagtaa ttttggtgtt gtgaaactga atttcaaata tttagtcggt atccctttaa
    49801 ctgccttaag ttacctggct ttcggccagg aagacaaaaa ggtggatgtt gaagaacttt
    49861 ttcgtataga cacgccaata actattgaat tggaggaaga tgaatttgag cgtgttgaac
    49921 ctaaaaagaa aaagcaaaaa aggaatacat tttttggcat caaaacaaaa aaaggattta
    49981 ctaaaaatgg ctttggtgat aacgtggtac tggaactatt ctattacctg aaagacccaa
    50041 taaaaattga tccttatgta aatgaaatat attggtttga tttcagaagg aaagcaattc
    50101 gagccggtaa gaatgtggat cctaaatatg gagtggtgct acatggccct tataagaaaa
    50161 tgaagggaaa gcaagttctt gaggaaggat attattatga aggggctaag catggaaggt
    50221 ggactactta taacaacgat aatatattaa ttaccaagac caaatatcat aaaggctggt
    50281 acaaggagtc cctggtttcc ttttatgatc tggagcatac tcaattgcga gaagtaatac
    50341 cagttcagta tggtaaacga catggagaat atttttactt tcatcctaat ggcaatattg
    50401 ctgtgtccgg aaaatatgag aacgacgtca agattggagt atggacagaa ttttatgaaa
    50461 taagaggaag aaggaaaaag atgattcaat atactgaaga ccactacaat aagaaattcc
    50521 aacctgttac tctaaaagaa tggaacgatc gaggtcagct tatttatgat cgcgaaactt
    50581 atggttttgg cttgccggat acgaactaga atgcatcccg gatatgaaag atggcgcact
    50641 ccttcttttt gtgaatggct ataatatcaa accggatatt acctttccaa ttctcagttt
    50701 ccaaatacaa ctccgcggct tccctgattc tctcttcctg atccccgcta accatctcct
    50761 ccggatatcc gtattttatt gttgatcggt attttacttc cacaaaaaca acaaaactat
    50821 ccattctcgc gatgatatca atttcatttc tcccagcccg atagtttgtc ctcagtatat
    50881 cataaccttt tgattttaag taatttatcg caagtaattc tccctctttc ccagcagagg
    50941 tcttcgacat taaatcacat ttattgttgt ttttatgcaa tttaaagcaa aaagatgata
    51001 cgtttccggt cagtatcaat gtaattttca atttcagctt catactaagc ccttcacgat
    51061 gttcccggct atatttacag ttgaaatgaa acccggtaaa atatcgatgc ttccaaagaa
    51121 aaatccaact caaaccaagg catggaaaga attgaaatcc cactttcaaa aggtggcgcc
    51181 actgaggatg gtgaatatgt ttaaagaaaa tccaaatcga gcgaaaagat tcacgctgca
    51241 acacgatggc ttgcttctgg atttttcaaa aaacagaatt actgaagaaa caatggatct
    51301 cttaattgac ctggctaacg aagttcaact gaagcaagct attcaagcta tgttcacagg
    51361 agcaaagatt aatgaaactg aaaataggtc agtcctgcat actgctttga gaaaaagtac
    51421 ggataataat cttgaaattg acggattaca catttccgag gccgtagaaa atgtattaca
    51481 aaaggtcgaa aaattttcga ataaaataac aaccggaaac tggaaaggat tttcaggaaa
    51541 acgaattagc gatattgtta acattggcat cggaggttct gatttgggtc ccgcaatggt
    51601 ttgtgaagca ttaacacctt acaaaatcaa aaatattaat acccattttg tctccaatat
    51661 tgatagttcg catcttacgg aaacaattaa aaaagttaat gcggaaacca cgttgtttgt
    51721 aatttcttca aaaactttta caactcagga aactatgacc aacgcctggt cagcgagaaa
    51781 atggtttctg gaatcagcaa ccgaagcaga catagccaaa cactttgttg cagtttctac
    51841 caataaagcg gcaacttcgc aatttggcat cgaagagtct aatgtttttg aattttggga
    51901 ttgggtaggt ggcaggtatt cggtttggtc agcgattgga ttaaccgttg cctgcgggtt
    51961 aggctttgag aatttcaaaa agtttcttga tggtgccgct tccatggata aacatttttt
    52021 ggataaaccc ttccgtgaaa atattcctgt tattcttgca ttgattagca tctggtatgt
    52081 gaattttttt aattgcacta gtgaagcaat cctgccctat gatcagtatt ttgataagct
    52141 ggttgattat ttgcaacaag taatcatgga aagtaatggt aaatctgttg atcgtaatgg
    52201 cgaaagagta agctataata cttgtccggt tatttgggga gctccgggta caaatagcca
    52261 acattcattt tttcagctgc ttcaccaggg tagtcaagtc ataccatgcg attttattac
    52321 cactatgaaa tctcagaatc ctttgggcga tcataagagg aaactattag ccaatttttt
    52381 tggacaaaca cgggctttaa tggttggcaa gtcgcgggag gaagtaagtg aagagatgca
    52441 aaggatggga tttactgatt cagaaataga gagaattggt ccacataagg tatttgaggg
    52501 aaatcggcca agcaacagta ttatcattga agaactaaat ccatattcac ttggaatgct
    52561 gctggccatg tatgagcata aagtatttgt gcagggtatt gtatggaata ttttcagttt
    52621 tgaccaatgg ggtgttgaat tgggaaagca aattgccaaa ttaatattac ctgccctgga
    52681 aaaggataat gagataagca ccgacggcag tactcaatca ttaatttcac attttaagaa
    52741 tatagccaaa caatagatgg cagaatttta tccgttgaag tttcatccga ttttcaaatc
    52801 aaaaatttgg ggtggacaaa agattaaaac catattaggt aaagattttg gtcagctcaa
    52861 aaactgcgga gagtcgtggg aattatcttg tgtggaagga aatataagta ttatcgcaaa
    52921 tggaattttt gcgggcagag cgcttgattc tctcattcag ctatacccaa acgagataat
    52981 gggctcccgt attaagttcg aaaattttcc tttgctaata aagttccttg atgccaatga
    53041 tgatctttcc atccaggtac atcccaatga caaacttgcc aggaagaagc ataatggata
    53101 tggcaagacg gaaatgtggt atattattga tgctgatcat ggcgcccgca tgtatgtagg
    53161 tttcaacaga aatttaacca gggaagaatt cattcaaaaa gcagaggctg gtgaagttac
    53221 ggattgggtt tgcactgagg aggttatgcc cggtgatact ttttatattc cggcaggaag
    53281 aattcattcc atcggaaagg gaattttact ggctgaaatt caacaaacct ctgacattac
    53341 ttaccggatt tttgatttta accgaatggg tgatgatggc aaaccacggg acctgcatat
    53401 ttccaatgca gcggacgcct taaactttaa cgatgtcgga accactttca aaacaaaata
    53461 taatgctacc gaaccttatg ctgttttggt gaaatcccct tacttcacta caattcggct
    53521 tacgggagag aaatcactaa aagtaaatct gaacaatgag aattctttcc ggatttacat
    53581 tgttattaat ggagaaggcc aaattaattg ggctggtaat tcgatgacaa tttctcttgg
    53641 agaagttatt ctgatacccg caagtatccg ggaatatgag ctaagaacag gaatgaattt
    53701 ttgtggtttg gaagtttaca tttcatagct gtgtacacaa cgaaaaacaa gaaggttaag
    53761 atcattgatc tgggactaat ggattataga gaatcctggc aataccagga gtcggtcttt
    53821 aaagatgtga tagataaaaa aacaaagaat cgcagtctgc ctgtaaattc acaggtgctg
    53881 gtccctaatt tcttaatttt tgtggagcac ccacatgtat ataccatggg caaaagtggt
    53941 gatgaaaaga atcttttgct aaatgaatca caactcaaag agaaaaatgc tcagtattac
    54001 aagattaacc gcggcggaga tatcacctat cacggacctg gccagattgt agggtatcca
    54061 attctcgatt tagataattt ctttacagac atccacaaat atctccgaat gttggaagaa
    54121 gcagttattc gcacactgat gaattacgga ttggaagcag gaagaattga tggattaacc
    54181 ggcgtctggt ttgatcatat ctctttgaaa aaccccagaa agatttgtgc tatcggagta
    54241 aaatcaagca gatgggttac tatgcacggt ttctccttta atatcaacac agatctccaa
    54301 tacttcaact atataattcc ctgtggtatt aacgacaaag cagttacaag tttgtcaaag
    54361 gaattgggtg caaagcaaaa tttagaggag gtaaaaataa ttctcaccaa acatttagta
    54421 gatttgtttg aaatggagat tatttaattc atgaacatac aggacttaaa aaatatttta
    54481 cttattgata tcgaaacggt cggtatggct gaaaccttcg atcaactgca tccccggttc
    54541 caaaaagaat gggaaaggaa atctaaattt ctgctaaaca gcgataccat gacgccgcag
    54601 gaattatttg aggaaagagc aggaatttat tctgagtttg gtaaaattgt ggtaattggc
    54661 ctggggttat ttaacttatc accagaaaaa gaattgtctc tgagagttaa ggctcttgcc
    54721 ggcgttgatg aaaagaaact ccttgaggat ttcaaacatc taattgaatc gaaattttct
    54781 gctgatactc tttccctttg tgcacataat ggaaaagaat ttgattttcc ttatattatc
    54841 cggagaatgt tggttcatgg gataacactt ccgggtgcta tggattattc cggaaagaaa
    54901 ccatgggaaa tcaggcatat tgatactatg gatttgtgga aatttggtga cagaaaaagt
    54961 ttcacttctc tggaacttct tgccgcttta tttggaattg attccagcaa gaatggaatt
    55021 gacggcagta aagtaaacga aaccttttac aaggaaaaaa atctggataa aataattgaa
    55081 tattgcaagg aagatatcgt tgttacagga caagtatttc taagactaaa tgccctgcct
    55141 tcaatcaaaa atgaaaacat acaccttctg taacaggaat tcaatacaac agaatgggca
    55201 ggaagaaaaa aaaggatcat agcaataaga aaattaagaa ggtcagaaga tcagatatag
    55261 cgaatgacat acaacaactt cttaattcca acctttcatc agccctatct ataaaacaaa
    55321 taaccaagaa attaggctac cgcgacaaga tccagaagaa tatggtgccc gaaactctat
    55381 ttgacctgga gaggtccggt tcagtgcagc ggcttcgcaa tggcagtttc aaaagtacac
    55441 gagaagctaa actgctaacc ggagttgttg accatgttaa tcccaagttc gcttacataa
    55501 tatctgagga aagtgaagac gatatctggg tccgaactga tgatcttaat tttgccatca
    55561 atggagatac ggtcagtgtc caggtttatc ctcaaaaaca cggaaaaaaa ccggaaggaa
    55621 aagtaaagca aattatccaa cgcggaaaag atgagtgggt tggcagagta gaaatttcaa
    55681 aaaattatgc gtttgtaata cccgacaata agagaatcta ttgtgatgtt tttattgctt
    55741 ccgaagatat taataaggcc agaagtcgcg ataaggtaat cgtaaaaata ttaaactggc
    55801 cttcagccga aaagaagaat ccgaaaggag aaataatcag agttcttggc cctgccggag
    55861 aaaatgaagc ggagatccat tcaatcatgg cacaatttga gttaccattt gagttccccg
    55921 ataaagtatt agcagaaagt gaaagaattc cggaaaaaat ctccagagat gaaataagct
    55981 cccgaagaga tttccgaaat attttaactt ttaccataga tcccgaagat gccaaggatt
    56041 ttgatgatgc gatttcattg caaacactgg agaatggtaa cacggaaatt ggagtgcaca
    56101 ttgctgatgt tacccatttc ctccgggaag ataccgttct tgacaaagaa gcctttgacc
    56161 gggctacctc cgtatatctg gtggatcgga ccatacccat gcttcccgaa cgattatcca
    56221 atggagtctg ctccctgaat cccaacgaag acaaattaac tttttcagcg gtctttgaaa
    56281 tggatgataa ggccaccgtg aaaaggcagt ggtttggaaa aaccatcatc cattcagaca
    56341 aacggtttgc ttatgaacaa gcccaggaga ttatagaaag cggcagtgga gagttcagtg
    56401 atgaactggt taaattaaat gagcttgcca ggaaattaag aaaggatcgt ttcgccaatg
    56461 gtgcgataaa ttttgaaacc attgaagtaa aattcaggct tgatgaaaaa gggaagccac
    56521 tggaagtgta tccaaaggag cgaaaagatg cacataagtt gattgaagaa tttatgttac
    56581 tggccaataa aaaggtggct gaattcatct ttaaaaagaa atccggagga aacaagtcat
    56641 cgacttttgt ttatcgcacc catgattcgc ctgatccgga caaaattaaa gccttctcaa
    56701 tctttgccag aaaattcggg tatcagatga aactcgaaga aagttccatc agtagatcac
    56761 ttaactcttt gatcgatgcc attgaaggca aacctgagca aaatgtgctg gaaagtctgg
    56821 ctatccgcac gatggcaaaa gctaaatata ctactgagcc tgatcctcat ttcggtctgg
    56881 cctttatgca ttacactcac tttacaagtc ctatccgccg gtatcctgat gtgatggttc
    56941 accggttact ttttctttat ctgcagaatg ggaaatcccc tgacgaggaa gagtatgaag
    57001 ataaatgtga acacagcagc gaaagggaaa aaagagctgc cgaggctgaa cgtgcctcaa
    57061 ttaaatataa acaggttgag tttatgcaaa cccggttggg tgaagagttc gatggcatcg
    57121 tcagtggcgt taccgaatgg ggcgtattcg tggagatgat cgaaaccaaa tgtgaaggaa
    57181 tgatccggat ctcggaaatt agagacgatt tttatgagtt tgatgaagag aatttcagga
    57241 tagtaggagt acgcaataaa cgcataatca ctcttggtga tgatattaaa gttaaagtag
    57301 ttaaaactga tatcgacaga cgaaccatcg atctggaaat gatcaattga ataatgctga
    57361 aagaatttca ggaagccatt gaaaaagaga taaattccct cacgttccag aaacctgggg
    57421 aattatacga gccaattcta tatatcatgc agttgggcgg aaagcgatta agacccttgc
    57481 tttgccttct ctcttacaac ttattcgctc aggaatggaa aaagatccta aagccggcac
    57541 tagcacttga aatatttcac aatttcacct taatgcacga cgacatcatg gataatgctc
    57601 caatacggag aggtaaacaa acaatccaca aaaaatggaa ttccaatgtc ggcattttat
    57661 caggggatgt aatgctggta aaagcctatg aacattttac caccgtggaa ggcaagtatc
    57721 ttctcagtgt tctgaattca tttaataaat gtgcaacccg ggtgtgtgaa ggccagcaaa
    57781 tggacatgaa ctttgaaact cagccggctg tttctgagaa gaattatctt tcaatgattg
    57841 aaaaaaaaac ggcagccttg cttggattta gcatggaatt gggcggtatt cttggtggtc
    57901 aaaatagcga ggtaaccgaa aaactttaca actatggccg caatcttgga ctggcttttc
    57961 aactgaagga tgatctcctc gatgtgtttg gggatgaaca atttggtaaa caatccgggg
    58021 gtgatatcat cagcaataag aaaacctttc ttctgataaa agtcctgcaa aaggcatcag
    58081 gtaccgatag gaaaacactc ttgtattggc tggatcaaaa agaatttgat agcgaagaga
    58141 aagtcaaaga agtgaaagca atttataccc gcctgaaaat cagggaagaa gcggaaagca
    58201 gaattaatgg ctactttgat ctagcaacag aaaatctccg gtattttaaa gcaaataaaa
    58261 atgcctggga attattattt agtttcactg gcaatttgaa aagtcgcgaa aaatgagtca
    58321 aacggtaata ctaatgatca ttattgggtt ggttagcttt gtagcctgga aacagcccgc
    58381 caaaatggaa aaatggattc ttaatcctta taaggctttt cacaataagg aatattttcg
    58441 atttattacc agtgggttta ttcatgccaa ttggggtcac cttattttca atctttttac
    58501 attatttttc tttggaagaa atgtggagta tacctttatt ggtttaatgg gtcctgctac
    58561 cggaatcctc gccttttgga tcctgtttat tactgctgta gtagtttccg atcttccctc
    58621 cttctttaaa tttcgaaata ttcctaacta caattccctg ggtgcttcag gcggagtttc
    58681 tgcattcgtt ttctcaagca ttatatttta tcccttacag cctatatata tcatgttcat
    58741 tccgattgga atccccggat ttattttggg cgctttgtac ctgctttatt cctattatca
    58801 gggaaaacaa cttggcgaca acattaacca tgacgcccac ttatttggcg cactttacgg
    58861 agttttgttt acagtaattg tgttcccaca aagcatttct ttattttttg aacagattaa
    58921 aaatttcagt ttctagcgaa aaaattagta tttaaatcgc atccccccgg catgtgtatt
    58981 ggaaggagaa aataaatgat tccgattact atgtatgttt tattgcatat ttcaaatatt
    59041 atatattaat attattggta tcattacagc ttgaaaatca gcaaatttta gggctgcgtt
    59101 cctattgaaa atcaataagt aaaggtgtat aaactcatag tagatgccaa caagccatct
    59161 tttgggctga attttaaaga gttatgggca tatcgcgacc tcattcttat cttaatttat
    59221 cgtgatttac gagttagata tacgcaaaca tttcttggat tatcctgggc gattattcaa
    59281 ccgcttgcaa cattattaat ctttactatg gtttttgaaa gggcaattgg aatagataca
    59341 ggtgatattc cttatccatt atttgcgtta tgcggtatgt cggcatggac ctactttgca
    59401 tttgtgttga ataattccgg gaattcaatt atttcttccc aggaaatgat taagaaaatt
    59461 tattttccgc gccttatcat tcctttaagt aaatctatgg tggggcttgt tgattttata
    59521 attgcacttc tgctaattgg ttgttttatg atcttctatg ggattactcc tggcaataaa
    59581 ttcttgtact tatcaatttt tatcgcgctt actattattt ctgcattaag cgtcggtatc
    59641 tggatcagcg ccttaacaat tcgttaccgg gacttgcaac atgtagttcc ctttcttgtt
    59701 cagtttgggt tatatgcaac accggttgga tatccctctg aattaattgt taagaatttg
    59761 ccggattggg cagtcacttt attttatctg aacccaatgg caggtatagt tgaaggtttc
    59821 aggtggacaa ttctgggagg ttctcccccc agtgtaaatg tgtattactc ttttggcgtt
    59881 gcaatattac tatttatttc aggtctctat ttttttaaga aagttgaacg ggtgatggcc
    59941 gatattatat gactgatttt gcaatcaaag taaggggttt aggaaaaaga ttccatacgg
    60001 gcaaaagcaa gagtgcagac tttcgcactt ctattggtta tcagataaaa tccatattta
    60061 gggttgggaa aatggagaag gagagtttct gggcattgca ggatattaac tttgaaattg
    60121 cacgcggtga gtctctggga ataattggct gtaatggcgc ggggaaaagt acgttgctaa
    60181 aaatattgtc aagaataaca gcgccaacaa cgggaatgtt tgaaattaat ggaagagtgt
    60241 cttccctgct ggagattgga acaggttttc acatggaatt aacaggcaga gaaaacattt
    60301 acctgaacgg aactatttta gggatgaaga gaaaagagat tagaaaaaag tttgatgaaa
    60361 tcgtatcctt cagtggcgta gaaaaattcc ttgatactcc tgttaaacat tacagttccg
    60421 gaatgaaagt gcgattagcg tttagtgtgg ctgcacattt ggaaccggag atacttatcg
    60481 tggatgaagt tctggcggtt ggcgatgccg cgtttcagaa aaaatgtctt ggcaaaatga
    60541 gcgaagttac cagaaatgaa ggcagaacta tattgtttgt cagtcataac atgaatgcgc
    60601 taaaaagcat ttgttcaaaa gggatcgtca tcagaaatgg taaaatagat ttgatatcag
    60661 atatctcaaa ttcaataaac cgttattcag ctaatgaaga aactttaacc ttttcaagaa
    60721 ctttgaagaa tgcttttatc ggtgatggta tttatattga agatttcact ctttcaaaac
    60781 atacctttga agtgttttct gatatggaaa ttgaattgaa gatgagattc ctcgaggaaa
    60841 atatggtttc agagttggct ctattaatca ataacgaaac tggagaaagg gtagccatca
    60901 ttgatatgag agaatattta gaaggcaaga aaacaagatc tttggaattt cagggagtaa
    60961 ttaataaaat gccgttgttg gaaggtcatt attcgatagg gttatatctt ctttcaaacc
    61021 ttgcaacagg aaattaccag aaactcatca aattctccat cactgggaaa gaaactaaaa
    61081 tcccttcatt tcccttaaat gtgcgaggtt acgtggagtt agaaaataaa ttcaattaaa
    61141 tggaaaagta tttttctcaa tttggacaag acgaatattt ggacaaaaaa atttttaaga
    61201 aaagagaaaa aggtttcttt attgatatcg gagctcatga tggaatcacc tacagtaata
    61261 gccttttttt tgaaaggtac aggaaatggg ctggaatatg cattgaacct aatcctgatg
    61321 tatttcttga aatggcaaaa cttcgttcca gcttgaatat tgaggcatgc atcgggacta
    61381 agcatggtga agtggatttt ctaaaagttt ccggttatgc a
//
LOCUS       DASWHX010000055         5369 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_407725, whole genome shotgun sequence.
ACCESSION   DASWHX010000055 DASWHX010000000
VERSION     DASWHX010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 5369)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5369)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5369
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_407725"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1027
                     /locus_tag="VGA21_04060"
     CDS             <1..1027
                     /locus_tag="VGA21_04060"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX9649708.1"
                     /translation="EYNDFISVIIACRNEEENITKIIESINLQDYPVKNFEVIIVDDH
                     STDKTPLLVAEFLDKTSFSLKLISLTGTTGKKSAISEGINNSQGEIIIITDADCKRKN
                     GWLRSMLNKHKSGNNDLSFGLVKFWESSSFFSKIVSIEQTSLVGAGAALWRLGVPVMS
                     NGANMIFSKCAFQQVDGYSGNLHIPSGDDEFLLSKFRAHNLKIGFVDDIDTVIETAAP
                     SGIVELLNQRKRWAGKWKASLSLSLKLVAIVVFFLQIFWLVSLIAFFAGKVELAYFLL
                     GMLLKAFFDFIFLKDISARLNKELHLLPFIFLEVVYPLYVIFFSLISNFGAYSWKGRK
                     YSHFINS"
     gene            1038..2264
                     /locus_tag="VGA21_04065"
     CDS             1038..2264
                     /locus_tag="VGA21_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009581955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HEX9649709.1"
                     /translation="MDKLTALQNKFNTKELELGSLLEITQAINNNLPEDALYKIFNFT
                     LRANLHIKKLVLYVHGEKWETKVQFGTQNNFSAIELPKHILKIQEPHKLDSVDGSEFN
                     EFDLIIPVLHKDKKLAFLLTQGVGGENGQAGQASTIYLQAITNIIIVAIENKRLARRQ
                     LEEEAFRKELEIAKDVQKFLFPENLPYGVRLKIEASYLPHHTVGGDYYDYIPINQNQF
                     LICIADVSGKGIPAAIIMSNFQASLRTLIRQTPNLSEIIEALNFQILEHAKGENFITF
                     FAAIYDHHLKTMVYVNSGHNHPILYDKENGIRLLDEGSTVLGVFHPIPFINEGFITEL
                     ENFLLFLYTDGVTEVSNEKGEEFGSDRLKEFILKNKDADLKEIHKLLISELERYKGKN
                     NYNDDITILSCKMQGS"
     gene            2267..2905
                     /locus_tag="VGA21_04070"
     CDS             2267..2905
                     /locus_tag="VGA21_04070"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX9649710.1"
                     /translation="MSRLYWHKTPPIFSKLFPSFLWHKSREEKTIFLTFDDGPIPEVT
                     DFILSELEEYGAKATFFCVGHNIEKYPDTFQRTIHFGHSIGNHTHNHLNARKVSPAEF
                     VDNISLCQSEINKQGYFRIHKLFRPPYGMLRKRNTSRIRENYQIVMWDVLSGDFDSRL
                     GQEKCLSNTIRSTENGSIIVFHDNEKSGRKIKYVLPRFLSYFAEREYKFKAI"
     gene            2902..4014
                     /locus_tag="VGA21_04075"
     CDS             2902..4014
                     /locus_tag="VGA21_04075"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX9649711.1"
                     /translation="MIFILVIVSIVILSELILLGSVLLLKDELFASEKEEFVSVLISA
                     RDEERFIGGVIQSLLNQDYNYDKFEILIGDDNSSDSTASVVKSFADKNKNVHCFPIKA
                     EDKRTGGKAGVLDILIEKTQGEHILIADADMVYPRSWIRAMSAHLAQGQLVSAFTKVE
                     GKGIWNQWQNLDWLLNLSGVKMLSKFGFDLTAIGNNMGFSKSSYRRIPGFGKMKGFLT
                     EDMDLLLQMKSVSVPAKFIFSKQTLGSTQPSENLTALIAQRKRWMSGAFKAPIWLIAG
                     WFLRVILFPTVLVLCVFHLEAGFLIFLIHMVLQCGVLLLLKDKLDIKISVIALITFDL
                     FYLFLNLVSLTGFLGRKKISWKGRLYSKNELMHTEV"
     gene            4016..4786
                     /locus_tag="VGA21_04080"
     CDS             4016..4786
                     /locus_tag="VGA21_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009581952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEX9649712.1"
                     /translation="MFNFIDSHAHIYLEEFDSDIDAVIRRSQAVGVNKVYMPNIDPGT
                     VARMNSLAAGYPEFCYPMMGLHPCSVDENFENNLTLIESNLRSKKYFGVGEIGTDLYW
                     EKKFWEQQKIAFQFQLKLAKEMKLPAIIHCRESLEETIGMVEQMKDENLNGVFHCFTG
                     SIEQANRISALGFYLGIGGVITFKNGGLDRIIPNIDLSGIILETDSPYLAPVPYRGKR
                     NEPSFISLIAERISNICGISIEEVSRVTTKNSQALFGT"
     gene            4783..>5369
                     /locus_tag="VGA21_04085"
     CDS             4783..>5369
                     /locus_tag="VGA21_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014021263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEX9649713.1"
                     /translation="MKYIKTAINTATPKKPTSSLLLIYTGGTMGMGYDESGTLVPFDF
                     DSILDKVPSLRTFQIQINVIAFDPPLDSSSIGPDHWALLATIIKEHYSSHDGFVILHG
                     TDTMAYTASALSFMLENLNKPVIFTGAQLPISARRSDARENLITAIELASAKAGRNPQ
                     VPEVCIYFNYLLFRGNRVKKVESNHFDAFRSENYPA"
BASE COUNT         1671 a          939 c         1109 g         1650 t
ORIGIN      
        1 cgaatataat gatttcattt ccgtaataat tgcatgtcga aatgaggaag aaaatattac
       61 taaaattata gaatctatta acctgcagga ttacccggtc aaaaattttg aagtaataat
      121 tgtagatgat cattcaaccg acaaaactcc gcttttagtt gcggaatttc ttgacaagac
      181 ttcctttagt ttgaaattga tttctttaac cggcacaacc gggaaaaaat ctgccatttc
      241 tgaaggcata aacaactctc aaggtgaaat tattatcatt actgatgcag actgtaagag
      301 aaaaaatggt tggctgagat ctatgctgaa taaacataaa tcggggaaca atgatttatc
      361 atttggtttg gtaaaatttt gggaatcgag cagtttcttt tcaaaaattg tatcgattga
      421 acaaacttcg ttggtaggcg caggggcagc attatggcga ctgggagttc cggttatgtc
      481 taacggagct aacatgattt ttagcaagtg tgcctttcag caggttgacg gttattcagg
      541 aaatcttcat attccttcag gggatgatga atttcttctg tcaaaattca gagctcataa
      601 tttaaagatt gggtttgttg acgatatcga taccgtgatt gagactgctg ctccatccgg
      661 gattgtggaa ctattgaatc agcgaaagag atgggctggt aaatggaaag cctctttaag
      721 tctgagtctt aaattagtgg caattgtagt tttctttctg cagatatttt ggttagtctc
      781 attaattgca ttttttgccg gaaaggtaga actagcatat tttttgcttg gaatgttgct
      841 taaggctttt tttgatttta tattcctgaa ggatatatcg gccaggttaa acaaggaatt
      901 gcatctcctg ccgtttattt tcctggaagt tgtgtatcca ctttacgtta tatttttttc
      961 tttgattagt aattttggag catacagttg gaaagggcga aaatattccc atttcatcaa
     1021 ttcttaatag aaacattttg gataagttaa ccgccttaca aaataaattc aacacaaaag
     1081 aattagagtt aggttctctt ctggaaatta cgcaggcaat caacaacaat ctccctgaag
     1141 atgcccttta taaaatattt aacttcacct taagagcgaa tttacatatc aagaaacttg
     1201 ttctttatgt tcatggagag aaatgggaaa cgaaagttca atttggaact caaaacaatt
     1261 tctccgcgat agagctcccc aaacacattc tcaaaattca ggaaccccat aaattggata
     1321 gcgtagatgg ttcggaattt aatgaatttg atttgataat tcccgttctt cacaaagaca
     1381 agaagctcgc gttccttctt acccagggag ttggaggtga aaacgggcaa gcgggtcagg
     1441 ccagcactat ctatctccag gcaataacca acataattat tgttgctatt gaaaataagc
     1501 ggttggcaag aagacaactt gaggaagaag cctttagaaa ggaattggaa attgcaaagg
     1561 atgtacaaaa atttcttttt cctgaaaatc taccttatgg cgttcgcctg aaaattgaag
     1621 ccagttatct gccgcatcac actgtgggcg gggattatta cgattacata cccatcaatc
     1681 agaaccaatt tcttatttgt attgcagatg tgagcggcaa aggaattcca gcggcaatta
     1741 taatgtctaa cttccaggct tctttgcgga ctttaatcag gcaaacaccc aacctatctg
     1801 agataataga agctctgaac tttcagatcc tggaacatgc caaaggggaa aactttatca
     1861 ctttttttgc cgcgatatac gatcatcatt tgaaaacaat ggtttatgtt aactctggcc
     1921 acaaccatcc catcttgtat gacaaggaaa atggaatcag actactggat gaaggttcta
     1981 cggttcttgg agtttttcat cccattccat ttattaatga aggattcatc actgaattgg
     2041 aaaactttct gttatttctg tatactgacg gagttaccga agtctccaat gaaaaaggag
     2101 aagaattcgg atcagatcgt ttaaaggaat ttattctgaa aaacaaagat gccgacctga
     2161 aagaaataca taaattattg ataagtgaat tggaaagata taaagggaaa aacaattata
     2221 atgatgatat tactatcctt tcgtgcaaaa tgcaaggttc ctgaatatgt caagattata
     2281 ctggcacaaa acgccaccta ttttttcaaa attgtttcct agttttttat ggcataagtc
     2341 aagggaggaa aagacaatat ttctgacttt tgacgatgga ccgattcccg aagtaactga
     2401 ttttatcctt tctgaactgg aagagtatgg agcgaaagca acattcttct gtgtaggaca
     2461 taacattgaa aaatacccgg acacttttca aagaaccata catttcggcc attcaattgg
     2521 aaatcatact cacaatcatt tgaacgcgcg aaaggtaagc cctgcagaat ttgttgataa
     2581 tatttcactt tgtcagagtg aaataaataa gcaaggctac ttccggattc ataaattatt
     2641 cagacctcct tatggcatgt tgcgaaaaag aaataccagc agaatccggg agaattatca
     2701 aatagtaatg tgggatgttc tttccggaga ttttgattcc aggcttggtc aggaaaagtg
     2761 tctgtcaaat acaatcagaa gtaccgaaaa cggatctata attgtctttc atgataatga
     2821 aaaatcagga agaaaaatta aatatgtttt gccgcgcttc ctgagctatt tcgcagagcg
     2881 ggaatacaaa ttcaaagcga tatgattttt attctggtta ttgtaagcat tgttatttta
     2941 tcagaattga ttcttcttgg ctcggttctt ttgcttaaag atgaactgtt tgcatctgaa
     3001 aaagaggaat ttgtgtcggt gttgatttct gcaagggatg aagagagatt tattggcggg
     3061 gttattcaaa gtcttctcaa tcaggattac aattatgaca aatttgagat ccttattggt
     3121 gatgataact ccagcgatag cacagcctca gtagtgaagt cgtttgcaga caaaaataag
     3181 aatgtgcatt gtttcccgat taaagcagaa gacaaacgaa cagggggtaa ggcaggagtt
     3241 ttagatattc tgatcgaaaa gactcaaggt gagcatatcc tgatagcgga tgctgatatg
     3301 gtttatccgc ggtcctggat tagggcaatg tctgcacacc tggcccaagg tcaattggtt
     3361 agcgcattta ctaaagttga agggaaggga atctggaatc agtggcaaaa tcttgattgg
     3421 ttgctgaatt tatccggagt taaaatgctc tcaaaatttg gctttgacct caccgcgatt
     3481 ggcaataata tgggtttttc aaaatcatcg taccggcgaa ttccaggctt tggaaaaatg
     3541 aaaggatttc tcaccgagga tatggattta cttttgcaaa tgaaatctgt ttctgttccc
     3601 gcgaaattca ttttctcaaa acaaacattg ggttcaactc aaccgagtga aaatctaact
     3661 gctttaattg cccaaaggaa gcggtggatg tcgggagctt ttaaagctcc aatttggtta
     3721 attgccggct ggttcctgag agtgatttta tttccaacgg tcctggtttt gtgtgttttc
     3781 catttggaag ccggctttct tattttctta attcacatgg ttctccaatg tggggtatta
     3841 ttactgctga aagacaagtt ggatattaag atttcggtta tagctcttat tacgttcgat
     3901 ttattttact tgtttcttaa cttagtaagc ctgacaggtt ttttagggag aaagaaaatt
     3961 agctggaagg gaagattata ttctaagaat gagttaatgc atacagaagt ttgaaatgtt
     4021 caatttcatt gatagtcatg cccatattta ccttgaagaa tttgattctg atattgatgc
     4081 cgtaattcga agatcacaag cggtcggtgt aaataaggtg tacatgccca acattgatcc
     4141 tggaacggtt gccagaatga attctctagc cgccggctat ccggaattct gttatccaat
     4201 gatgggcttg catccctgct cagttgatga aaattttgaa aacaacctca ctcttattga
     4261 gagcaatctt agaagtaaga aatattttgg cgttggcgaa atcggtacgg acttgtattg
     4321 ggaaaaaaaa ttctgggaac aacaaaagat agcttttcaa tttcaattaa agctggccaa
     4381 agaaatgaaa cttccggcaa ttattcactg cagggaatcc ctggaggaaa ctattgggat
     4441 ggtggaacag atgaaagatg aaaacctaaa tggagttttt cattgcttta ccggttcaat
     4501 agaacaagca aacagaattt ctgctttggg cttttacctg ggcataggcg gtgtcataac
     4561 ttttaaaaat ggcgggctgg atcgcataat tccaaatatt gatttgtctg gaatcatcct
     4621 ggaaacagac agcccttacc tggcacctgt tccctacagg ggaaaacgca acgagccatc
     4681 atttatctcc ttgattgccg aaagaatttc taatatttgt ggaatttcaa ttgaagaagt
     4741 gagtcgtgtt accacaaaaa attcacaagc cctttttgga acatgaaata tatcaaaact
     4801 gctattaata ccgcaacgcc aaaaaagcca acgtcttctc ttttgctgat ttacaccggc
     4861 gggacaatgg gaatggggta tgatgaatcc ggaactcttg tcccatttga ctttgatagt
     4921 attctagaca aagttccatc cttgagaact tttcaaattc agataaatgt catcgcattt
     4981 gatccacctt tagattcttc aagtattgga cccgatcatt gggcattact tgcaaccata
     5041 ataaaagaac actatagttc acacgatggg tttgtgattt tacatggaac cgatacaatg
     5101 gcgtatacgg cttcagctct aagctttatg ttggaaaatc ttaataaacc ggtaattttt
     5161 acaggggctc agcttcccat atccgcaaga agatcagatg ccagagaaaa tttgattacc
     5221 gccattgagt tagcttctgc aaaagcgggt cgtaatccgc aggtgcccga ggtctgtatt
     5281 tatttcaatt acttactctt tcgcggtaac agggtgaaga aagtggaaag caatcatttt
     5341 gacgcatttc gatcagaaaa ttatccggc
//
LOCUS       DASWHX010000056         8020 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_1518027, whole genome shotgun sequence.
ACCESSION   DASWHX010000056 DASWHX010000000
VERSION     DASWHX010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 8020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8020
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1518027"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..964
                     /locus_tag="VGA21_04090"
     CDS             <1..964
                     /locus_tag="VGA21_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013453217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX9649714.1"
                     /translation="IPVSVESLKPRSISKYIETTGTVYSSQEGVMKSELAGLFMLQLN
                     PSTGKFYALGDIVRKGQVIVTLVDREFENNLQYDAKKLNLEVSKNNLDKQQSLYDKGG
                     VTLTELKNASIEYVNAKYSFENAQIQRDKMSVKAPFDGVIVKLPHHTIGVRIDQGQDL
                     FKVMNYDLLLMDIKLPEKYLQEIRINQLVHITNYNLPNDTLVGRITQISPVIDTETRT
                     FESVLKIENKKLQLRPGMFVKAAILSEKRDNTIVIPKETIISRQNGSVVFTVENGIAS
                     ERIISTGLENQDLIEVIEGLKVNDRLVISGFETLRDKSKVSVIR"
     gene            983..4123
                     /locus_tag="VGA21_04095"
     CDS             983..4123
                     /locus_tag="VGA21_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013453216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX9649715.1"
                     /translation="MKKIITLAVNYPVSILMLVLAVILLGSISFGKLGIELFPDLKNP
                     TLFIEMKAGIRPPSEMEKQFVEDIESVAIRQNGAVEVSSVCQTGYARVTVHYDLGKDM
                     DEAFLDLQKALSTFSTNEDIEEFAITQFDPNASPVMILGVYNPESDDFDALRKVAETN
                     FRNQLIRLPGIAEVKISGAQDKEVVIETDPHQLASFGLTVDDLVMRIQSYNRNVSGGF
                     IEELGKKYVIKGLGIIVKPEDLEELVVGYKQQDLPVGLPSQETPNPQTAVSDRVPLLL
                     REVATVNMLDKEASSIVRVNLKRSLGLSIFKETKYNTINAVNELTEVLEELQRTVPGY
                     EFVIIQNQGSFIQQAVNEVEESGLYGALFAVIVLFVFLRRLGATLIISLAIPVSIIAT
                     FNLMYFNGLTINVMTLGGLALGAGMLVDNAIVVVESIFRKREEGLNFREAAIQGTAYV
                     SGAITSSTLTTIIVFLPIVYLQGPSGELFKDQAWTVAFSLLSSLVVAILVIPMLFTQF
                     FRKQNQKSETKALHFRGYRNFLNKVLQYRFVFIVLSILLVAGSVWLIPVIGSEYMPSA
                     GSRAVSVDISLSNGTPLARTSKTIAQVEAQLYELFSGQVEKIYSHIGPQQSQSAVVND
                     NVYQENRANLKIIFNKESHFEMAGIINILSNYFVNIPGLEATFSNDESALNSITGEDE
                     MPLVVDIAGSEINILRRIADSVLIILNRNHDIYDPVSNLEEGTPQVQVDVDKYRAGLF
                     NLSIEEVLTQVQDHLQGKKAGTLEQDGIIQDIQIKIPDLALSDLENLKIKKDQTELRL
                     GEIADIMVNNSVNSIYRKNQDRIVTVGARVNDDRPYDQIVKSIEEEFGNISLPPQYKI
                     EVSGQEKRRKESMDNLIFSLMLSILLVYMVLASQFESLLHPFTILLTIPLAGVGGLAA
                     FYILGLPLNMMAVIGLIMLTGIAVNDSIILVDAINKNKNSGFPLREAILDAGQKRIRP
                     IIMTSLTTILALLPLTFGFGESAALRSPMAIAVIGGLVTSTLLTLVVIPCYYETIEKI
                     YQRMKNFGTRKATADA"
     gene            4116..>8020
                     /locus_tag="VGA21_04100"
     CDS             4116..>8020
                     /locus_tag="VGA21_04100"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX9649716.1"
                     /translation="MPNLGILGVIIKRKVLVSMIFVGLSLLGYISYKKLPIELFPNVE
                     LPILIIQVNSATDVDPKYMEQQAIIPLEGAVGTLEGIAEINSTANSRQGLIFISLEQD
                     VNIKYAYLKLEQKVNSVRASLGQDFLVNIIKIDTEQLTNVFMSVQVLGSGGVDRVRNI
                     TDRYIVNRLANIDGMGGVEVFGGRQKTLEIIMNDALCEANGITPNAINDVLARNSNQS
                     AFAGIVRQNGNRVFVNVTAELKDIKNIQNLVVSEKGPLLLRDVAEVYFGVKEQASLSR
                     VNGKDAVSIRLSRDTQANVIALSEITSKVIDEINEDIAQLDAEIVVQSNNAEIMVNNI
                     EQIKSLALVGGLLAVFVLWIFLRRLRFILSIALAIPISVFTAFNLFYAYNISVNSLTL
                     IGMALAIGMLLDNSVVVIENIYRHLANKKSADAAVVEGTKEVMRSVIAATLTTITVFL
                     PFVFSSNFLITIIGTHIGVSIISTLIVSLVVALVLIPMLAHAAINKEKSLGSPLIKNS
                     SIRQRLVQIYVIFLKASFRKPALTIIGGVFLFFVTIGLSLIISFASINEVENKELKMF
                     VTMPEGSTLDNTDLKVRSIEEKVMRIPAVEKVISQISEGNSVITVQLVEEFSDLDTIS
                     IFDMKVRLSAISREYDDLEISLDKQPASSSQSRRPSSSMDFERLLGLGSEQERILISG
                     QDFELMEAFAHDINIFVSNLQSIENSSINVSPSRPEAHLIFNQVRMSDLGITQNDVAA
                     GLNDFQSSFTSGSRFVSGDEEYEITIRTENKETPRTKGFKELTVMPVVNAEGATHELS
                     DFSAVIYANGDSEIIRVNQEKQIEITYSFIEEVLASEDLLEASRIEIDQIVNGVNIPS
                     GLNVDVIHEEQLFDDFYFLITAALILILMILASVFESLYLPIVIMFTIPLSAIGAFLG
                     LTLTGNSLLNANTLVGFLILLGVVVNNGIILIDYSRILRHRGYSKYRALMMSGISRLR
                     PILITSITTIVAMLPLAMGEAEYVVNIGQPFAITVMGGLAFSTVLTLVYIPTMSASLE
                     NVIDWFRNLNPIIKVIQFALLILIVFFIYTEINGLLWQLIWFVSGVILIPGGTYFVMT
                     ALKRAYSNMIKEGESMNIEIQHLTKVYGRDKQMIREWKGNKNLYDMRGERLVSLRLFF
                     QNLIWQGALAAFLAYFIYFYLDKAFWQLIFMLALHKLLLYIIDEIILLSAPARKKLAL
                     ILSRIVYWGFPVFSSIYIYQNIYVKAQGVILIVLWFIGLILVGAAGRHAAKPVKPDSV
                     KGRFKFMKRIYYRILNAIPFIGRKKEQFKALSGVSLSIG"
BASE COUNT         2353 a         1593 c         1773 g         2301 t
ORIGIN      
        1 aattcccgtg tcggttgaaa gcctgaagcc gcgatcaata tcaaaatata ttgaaacaac
       61 cggtaccgta tattcctccc aggaaggtgt gatgaaatct gagttagcgg ggttatttat
      121 gctccagctc aatccttcaa cgggcaaatt ttatgccttg ggtgatattg tcagaaaagg
      181 tcaggtaata gtaacgttgg tggacaggga atttgaaaac aatttacaat atgatgccaa
      241 gaaactgaac ctggaagttt ccaagaacaa tcttgacaaa caacagtccc tttacgacaa
      301 aggtggcgta acacttacag agttgaaaaa tgcttccatt gaatacgtca atgcaaagta
      361 ttcctttgaa aatgcccaaa tccaacgtga caaaatgagt gttaaagctc cttttgatgg
      421 ggtaatcgta aagctgccac atcacacaat tggtgttcgg atcgatcaag ggcaggatct
      481 tttcaaagta atgaattatg acttgttgct gatggacatt aaacttcctg aaaagtattt
      541 gcaggaaatc aggattaatc aactggttca cattactaac tataaccttc ccaacgacac
      601 cctggttgga agaattaccc agatctctcc cgtcatagat actgaaacga gaacatttga
      661 aagcgtttta aaaattgaaa ataagaaact gcaactaagg ccaggaatgt ttgttaaagc
      721 ggcaatcctt tcagaaaaac gagacaacac aatagttatt ccaaaggaaa ctatcatctc
      781 caggcaaaat ggcagtgtag tttttactgt ggaaaatgga attgcttcgg aaagaataat
      841 ctctaccggt cttgaaaacc aggatttaat tgaagtgata gaagggctga aagttaatga
      901 tcggcttgta atatccggtt ttgaaactct gcgtgataaa tcaaaggtta gtgtaattcg
      961 ataaaatcaa tttctattcc cgatgaaaaa aattattaca ctagctgtta attacccggt
     1021 ttccattttg atgctggtac tggcggttat cctattgggt tcaatttcat ttgggaaact
     1081 ggggatcgaa ttatttcctg acctgaagaa ccctacactc tttatagaaa tgaaggccgg
     1141 aatacgaccc ccgtctgaaa tggaaaagca gtttgtggag gacattgaat cagttgccat
     1201 caggcaaaat ggcgcagttg aagtaagttc agtttgtcag accggctatg cacgcgtaac
     1261 tgttcattat gatttgggga aagatatgga tgaagctttt ctcgatttgc aaaaagctct
     1321 ttccaccttt tcaaccaatg aggatataga ggaattcgcg attacccagt ttgatccaaa
     1381 tgccagcccg gtgatgatcc tgggagttta taatcctgag tctgatgatt ttgatgcact
     1441 cagaaaagtt gctgaaacca atttcaggaa tcaattaatt cggctgccag gaattgctga
     1501 agttaaaata tcaggtgcgc aggataaaga agtggttatt gaaacagacc cgcaccagct
     1561 ggcttcgttt ggtcttactg ttgatgacct ggtaatgaga attcaaagct ataatcgaaa
     1621 tgtatccggt ggattcattg aggagcttgg aaaaaagtat gtcatcaaag gtctgggaat
     1681 aatagttaaa cccgaagatc tcgaagaact cgtagttggt tataaacaac aggatctccc
     1741 agtgggttta cccagccagg aaacgcccaa cccgcaaacc gcagtttcag accgggtgcc
     1801 attactacta agagaagtgg caacggtcaa catgctggat aaggaagcaa gcagcatcgt
     1861 cagggtgaat ttgaaacgta gccttggcct gtcaatattt aaggaaacca aatacaatac
     1921 cataaatgca gttaatgaat tgacagaagt tctggaggaa ttgcagagga ctgttcccgg
     1981 gtatgaattt gtaattattc aaaaccaggg aagttttatt caacaggccg tgaatgaagt
     2041 tgaagagtca ggattatacg gtgctttatt cgcagttata gtgctgtttg tttttctgag
     2101 aagacttggc gcgacactta tcatcagcct ggcaatacct gtttcaataa ttgccacctt
     2161 caatttgatg tatttcaatg gtctcaccat caatgttatg accctcggcg gtctggcact
     2221 cggcgccgga atgctggttg acaatgcaat tgtggttgtg gaaagcattt tcagaaaaag
     2281 agaagaaggc ctgaatttca gagaagcagc catccagggt actgcctacg taagtggagc
     2341 cattacctct tcaaccttga cgactattat agtatttcta ccaatagtat atcttcaggg
     2401 accatcgggt gagttattca aagaccaggc ctggactgtt gctttttctt tgctttcgtc
     2461 tcttgtggtc gcaatcctgg taattccgat gctcttcacc caattcttta gaaaacaaaa
     2521 ccaaaaaagc gaaacaaagg cattgcactt caggggttat agaaattttt taaacaaggt
     2581 cttgcaatat cgtttcgttt ttattgttct ttcgattctt ttggttgccg gttctgtttg
     2641 gctgattcct gtaatcggga gcgaatatat gccatcggca ggatcaagag cagtttcggt
     2701 tgatattagc ctttcaaatg gaacaccatt ggctcgtact tctaaaacta tagcgcaggt
     2761 ggaagcgcaa ttgtacgaat tattctcagg ccaggtagaa aagatatatt cgcatattgg
     2821 cccgcagcaa agccagtccg cagtggtaaa tgataacgtg taccaggaaa acagggcaaa
     2881 cctgaagata atcttcaaca aagaatcgca ctttgaaatg gcgggcatca tcaatatcct
     2941 ttccaactat tttgttaaca ttcccgggct ggaagctaca ttttccaatg atgaaagtgc
     3001 tctgaattct ataacaggtg aagatgagat gccattggtc gttgacattg caggatcaga
     3061 aattaacatc ctgagacgta tagctgattc ggttttgatc atccttaaca gaaatcatga
     3121 tatttatgat ccagtctcaa accttgagga gggtactccc caggttcagg tggatgttga
     3181 taaataccgc gccggacttt tcaatttaag tatcgaagaa gtgctgaccc aggttcagga
     3241 tcacttgcag ggaaagaaag ccggaaccct tgagcaggat ggtataatac aggatatcca
     3301 aataaaaatt cctgacctcg cacttagtga tctggagaat ttaaaaataa agaaagacca
     3361 aactgaactc aggctgggtg agatagctga catcatggtg aataattcag tcaattccat
     3421 ttaccgcaaa aaccaggacc gaattgtaac ggttggcgct cgcgtcaatg acgaccggcc
     3481 ttatgatcag attgtaaaaa gtattgaaga agagtttgga aatatttccc ttccgcccca
     3541 atacaaaatt gaagtctccg gccaggagaa aagaagaaag gaatctatgg ataacctcat
     3601 tttttccctg atgctttcca ttcttttggt atacatggtt ctcgcctcgc agtttgaatc
     3661 tttgctacat ccttttacaa tcctcttaac gattccgctt gccggagtcg gaggcctggc
     3721 agccttttat attcttggcc tgcccttgaa catgatggcc gtgatcggtt tgatcatgtt
     3781 aaccggaatt gcagtaaatg attcaataat ccttgtggat gctataaata agaacaagaa
     3841 cagtggtttt ccactaaggg aagctatcct ggatgcaggt cagaaaagaa tcagacccat
     3901 catcatgacc agccttacaa ctattctcgc acttttaccc ctgacatttg ggtttggtga
     3961 aagtgctgct ctgcgatctc caatggctat cgccgtaatt ggcggacttg ttacctctac
     4021 ccttctgacg ctggtggtaa ttccatgcta ctacgaaacc attgaaaaga tttatcagcg
     4081 gatgaagaat tttggaacca gaaaagcaac tgctgatgcc taacctcggg attcttggag
     4141 ttatcataaa gaggaaggtc cttgtttcca tgatatttgt cgggctttcc ttgctgggct
     4201 atatctccta caagaaattg ccgatagagc tttttccaaa tgtagaatta cccattttaa
     4261 tcattcaggt taattcggca acagatgttg atccaaaata catggagcaa caggctatca
     4321 ttccgcttga aggagcggta ggtacgctgg agggcatcgc cgaaatcaat tcaacagcaa
     4381 acagccgcca gggccttatc tttatttctt tagaacagga tgtgaacatc aagtatgctt
     4441 acctgaagct tgagcaaaaa gtaaatagtg tcagggcaag tctcggccag gattttctgg
     4501 taaacatcat caagatcgac accgagcaat taaccaacgt cttcatgagc gttcaggttc
     4561 ttggaagtgg tggagtcgat cgggtacgca atattacaga caggtatatc gtcaacaggc
     4621 tggccaatat tgatggaatg ggcggtgttg aagtttttgg tggccgccaa aagacactgg
     4681 agataattat gaacgatgcc ctgtgcgaag cgaacggcat cacaccaaat gccataaatg
     4741 atgttcttgc ccgtaattca aaccagagtg cgtttgccgg gattgtgagg caaaacggaa
     4801 acagggtgtt cgtcaacgtt acagccgaat tgaaagacat aaagaacatt caaaatctgg
     4861 ttgtgtctga gaaaggaccc ttgctgctca gggatgttgc cgaagtttat tttggagtta
     4921 aggaacaagc ctctttaagc cgggttaacg gcaaagatgc cgtctcaatt cgactttcaa
     4981 gggataccca ggctaatgtt attgccttgt cagaaataac ttcaaaggtt attgatgaaa
     5041 tcaatgagga tattgcgcaa ttggatgcag agattgtggt gcaaagcaat aatgcggaga
     5101 taatggtcaa caacatcgag caaataaaaa gtcttgcttt ggttggcggg ctattggctg
     5161 tttttgttct gtggattttt ctacggcgtc taagattcat cctaagcatt gcccttgcaa
     5221 ttccgatttc agtgtttaca gcttttaatc ttttctacgc atacaacatt tcggtaaaca
     5281 gcctgacctt gattggtatg gctctggcaa tcggaatgct tttggataac agcgttgtgg
     5341 ttattgaaaa tatttaccgg catcttgcta ataagaaatc tgctgatgcc gccgtagtgg
     5401 aaggaacaaa agaggtgatg cggtctgtaa ttgcggctac actaacaact attaccgtat
     5461 ttctcccttt tgtcttttct tcaaattttc tcattacaat cattggtaca cacattggcg
     5521 tctcaattat ttctactttg attgtctccc tggttgttgc cctggtgctc attccaatgc
     5581 ttgcgcacgc cgcaattaac aaagagaaga gtttgggttc gccattaatt aagaattcat
     5641 caatccgaca gcggcttgtt caaatttatg tgatcttcct gaaagccagc ttccgtaagc
     5701 ctgccctgac tatcattggc ggcgtcttct tattctttgt aaccattgga ttaagtctga
     5761 tcatcagctt tgccagcata aatgaagttg agaacaaaga actaaaaatg tttgtcacca
     5821 tgcccgaagg ctccacgctt gataacactg atttgaaggt tagaagtatt gaggaaaagg
     5881 taatgagaat acctgctgtg gaaaaagtaa tcagccagat ttccgaagga aattcagtaa
     5941 taaccgtgca actggtggaa gaatttagtg acctggatac catttcaata ttcgatatga
     6001 aagtccggct ttctgccatc tccagggaat atgatgatct ggagatttcc ctcgataagc
     6061 agccggcttc aagttcacaa tccaggagac cttccagttc catggacttt gaaagactcc
     6121 tcggtttagg atctgagcaa gaaaggatcc ttatctccgg acaggatttc gagctgatgg
     6181 aggcctttgc acatgatatc aacatttttg tgagcaatct tcaatccata gaaaacagct
     6241 caatcaatgt aagtcctagc cggcccgaag cgcatttgat tttcaatcaa gtgcgaatga
     6301 gtgacctggg aattacacaa aatgacgtcg ctgccggcct gaatgatttc cagtcctcat
     6361 ttacctcggg atcaagattt gttagcggag atgaggagta cgaaatcaca atccgtacgg
     6421 agaacaagga aactcccaga acgaaaggtt ttaaggaact aacggtgatg ccggttgtaa
     6481 atgcagaagg tgcaacccat gaactttcgg atttcagcgc tgtaatttat gcaaacggag
     6541 atagtgaaat catacgggta aaccaggaaa agcaaattga aataacctac agtttcatcg
     6601 aggaagtgct tgcttcagaa gacttacttg aagcctccag gatagaaatc gatcaaattg
     6661 tgaacggtgt taatattcct tcaggattaa acgttgatgt tattcatgaa gaacaacttt
     6721 ttgatgactt ctatttcctc ataactgccg cactgatcct aattctaatg atcctggctt
     6781 ctgtatttga atcactctat ttgccaattg tgatcatgtt tacaatacct ctttccgcaa
     6841 taggtgcatt tcttggcctt accttaacag gcaactcatt attaaatgca aacacactgg
     6901 ttggttttct tattctgctc ggagtagtcg ttaacaacgg tatcattcta attgactact
     6961 ccaggattct ccgacacagg ggctattcaa aatatcgtgc attgatgatg agtggcattt
     7021 cgagattaag acctattttg attacttcaa ttacgacgat tgttgccatg ctccccctgg
     7081 caatgggtga agctgagtat gtagtaaata ttggtcagcc ctttgccatt accgtaatgg
     7141 gtggccttgc cttcagtaca gtactcacgc ttgtttacat ccctaccatg agcgcaagcc
     7201 ttgaaaatgt tattgactgg ttcaggaact tgaatccaat catcaaagta attcagtttg
     7261 cgcttttaat cctcatcgtt ttcttcatct acactgaaat taatggcctg ctctggcagt
     7321 tgatttggtt tgtgtcggga gtaatcctta ttccgggcgg aacctatttt gtaatgaccg
     7381 cactgaagcg ggcatattca aatatgatta aggaaggtga atccatgaat attgagattc
     7441 agcacctaac caaagtctac ggccgtgaca aacaaatgat aagggaatgg aaaggaaata
     7501 agaatttgta tgatatgaga ggggaaagac tggtttcctt acgacttttt tttcaaaatc
     7561 tgatttggca gggcgctctt gcagcctttc tggcatactt tatctatttc tacctggaca
     7621 aagcattctg gcagttgatc ttcatgctgg ccctccacaa gctgctgctg tatattattg
     7681 atgaaattat cttgctgagt gccccggcca ggaaaaagct tgctttaatt ctaagtagaa
     7741 ttgtttattg gggctttccg gtatttagtt ccatttatat ttatcagaac atctacgtga
     7801 aagcccaggg tgtaatcctg attgttttgt ggttcatagg attgatcctg gtgggtgcag
     7861 ccgggagaca tgcggccaaa cctgtaaaac cagactcggt taaaggtaga ttcaaattca
     7921 tgaagcggat ttattaccgg atactgaatg caattccatt cattggccgt aagaaagaac
     7981 agtttaaggc actttcagga gtgagtttat ctattggtca
//
LOCUS       DASWHX010000057        19346 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_788662, whole genome shotgun sequence.
ACCESSION   DASWHX010000057 DASWHX010000000
VERSION     DASWHX010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 19346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19346
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_788662"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(134..532)
                     /locus_tag="VGA21_04105"
     CDS             complement(134..532)
                     /locus_tag="VGA21_04105"
                     /inference="COORDINATES: protein motif:HMM:NF014366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649717.1"
                     /translation="MEAENLGKTYKKKMPIFWWTHRWVHIKFISRELTSMFVAFYSIL
                     FLFFINAVSSGPTEYDAFLEKLESGGFIAFHIIAFLILIFHSVTWFNLAPKAMVVKIG
                     KQRVPGVVVALSNYAGWVFISILIIWLVIK"
     gene            complement(540..1283)
                     /locus_tag="VGA21_04110"
     CDS             complement(540..1283)
                     /locus_tag="VGA21_04110"
                     /inference="COORDINATES: protein motif:HMM:NF009051.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HEX9649718.1"
                     /translation="MKDTITLEVFRYRPEEDPEPAFQNYEVPLKKDWVILDALNYIKD
                     QIDGTLSFRWSCRMGVCGSCGMVVNGTPVLTCATFIEKFAPGPIRIEPLTNFPVVRDL
                     IIEMTDFMDKLKAVKPWIIREKEDTELKQEYLQTPDQLDDYKQYSMCINCMLCYSACP
                     VYGLEPEFIGPAAIALAQRYNMDNRDSGSKERMEVLNSEEGLWQCTFVGECSAVCPKD
                     VDPAGAIQRYKLAGAANFWQSFLLPWSKK"
     gene            complement(1290..3053)
                     /gene="frdA"
                     /locus_tag="VGA21_04115"
     CDS             complement(1290..3053)
                     /gene="frdA"
                     /locus_tag="VGA21_04115"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: protein motif:HMM:NF006686.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HEX9649719.1"
                     /translation="MELTRHECDILIIGAGGAGLRAAIAAAEENPNLSVGIISKVYPM
                     RSHTVSAEGGAAAVIKSTDSPDLHAYDTISGGDWMSDQDAVEAFVNEAPKEMIRLEHW
                     GCPWSRMDDGSVAVRPFGGMKTERTWFAADKTGFHMLHTLFQTTLKYNKLQRYDEWFV
                     TNLLVDEGRCQGAMAIELATGKIHYIAARAVIVCTGGCGKVFPFTTNAAIKTGDGMAL
                     AYRAGVPLKDMEFVQYHPTGLPFTGILITEAARSEGGYMINKDGYRYLQDYDLGTPQP
                     KPVLRTMELGPRDRLSQAFVKEQEKGRTLAGPYGHYVHLDIRHLGEKIINSKIPFVRE
                     LCLKYENIDPVKEMIPVRPVVHYMMGGIDTNIEGATKLEGLYAAGETACVSINGANRL
                     GSNSLTELLVFGARAGQNAANFATTKGRFSSALPSLATDEVKRLKENYLEKKEGSEKI
                     ANLRKEMHVCMESGAGIYRTEDSLKGSQAIVMELQERFKNIIISDRSSTFNTELTSAI
                     ELSHLLDIAEVVIQSALHRKESRGSHQRTDYAARDDKNFLAHSLAFRKENAPPDIQYA
                     PVTITRWPPAERVYGRETKTN"
     gene            complement(3331..3861)
                     /locus_tag="VGA21_04120"
     CDS             complement(3331..3861)
                     /locus_tag="VGA21_04120"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX9649720.1"
                     /translation="MTREEIIGQLQDSYKSFTAFVSSLTEKQFLFSPAPDKWTSGQQA
                     KHLLMSIKPLNMALSLPDWFLKIYVGKANRPSKTYEELVEKYKYKLSQGGTASSPYIP
                     KEVPVESQRIITRQIRTNVDKLCKKLERYSEERLDTLILPHPLLGKVTLREMMYFTIH
                     HARHHQNSIEQNLHNK"
     gene            3962..5284
                     /locus_tag="VGA21_04125"
     CDS             3962..5284
                     /locus_tag="VGA21_04125"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEX9649721.1"
                     /translation="MDKDRGFKRAIKLPHATALVAGTIIGASIFVQPSEITGQVHSVG
                     GVFIVWIVAGILTFFGALVCAELSSIFTKTGGVYTYLKEAFNPAMGFLWGWAMFWSMH
                     TGIIAAVAVIFARYTGYFFPLNDLEIKLVAIILILFHSAVNYFGVKHGSMLQTLVTAG
                     KVLVIGIIILAGFAIGSKLPNHFEAGSFESMSTSPGDILSALIAGLFAFGGWHMVTYN
                     SEETVDAKRTIPKALLWGTLIVTVCYLALNTVYLYVLPLDKVASSTKIAADTADYLMG
                     SGGGMFMAGLVMFSTFGSISGIILTGPRVYYSMAQDGLLFKWVGVLHSRYGTPHRAIF
                     LQAIWASILVFTGTYRELFTRVIYTEWIFFGLMAIGLILLRKRKDISRGYSIFGYPWI
                     PVIFIICCFTIVFNHILNEPVESLTGLSYVLAGLVVYFFLKFNKKSIT"
     gene            5281..6258
                     /locus_tag="VGA21_04130"
     CDS             5281..6258
                     /locus_tag="VGA21_04130"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX9649722.1"
                     /translation="MIIDFHNHFYPPAYIDAIKKNSSFYKVTYDEDENPVIHSPGDYN
                     IVVPGHRLVEVRSKVLKDAEVDMQVLSFTTPGTIVEPAERSVELARMVNDTFGEIIKT
                     NADQFTALATLPLNAPEACAGEMERAINEVGLKGVMLYSNANGVALADKRFWPMYEKA
                     NEMEAVLYIHPTYPVGVEAMEDYMLMPLIGFCFDTTLAAAKLVFEGIPRRYPKIKWVL
                     GHLGGAIPYLAERLDRGYEAFPECRVNIDQKPSEYLKKFYFDTVNFDLNALNLAVEFA
                     GVDQILAGSDYPHQIGSLKKMKESLKNLNISKSEMEMIQSENTKKLLGL"
     gene            complement(6255..7085)
                     /locus_tag="VGA21_04135"
     CDS             complement(6255..7085)
                     /locus_tag="VGA21_04135"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HEX9649723.1"
                     /translation="MLNYIRQSLRRKKARRVTQEYPPIVKTYHLENLGPIEFANWDNP
                     LAPEFVITQSNVDFFSKFIKEGDLVIDIGANFGDTTVPMGLSAGVGGLTLGFDPNPYV
                     FKILEKNSSLNYGKYNLVPLPYAISKEEAEFYFISSEASFANGGISPTKDSIHGKFIY
                     PEKIIGIKLSDFLEKEYPDWMSKLSFIKIDTEGYDKEIIKSISDLLAKYKPTLVAESF
                     TKNSDAEKIELYSVIEDLGYDIYYFSDFVMDAKIVPLKSKSDITLYKQTINIYATPKP
                     "
     gene            complement(7366..9954)
                     /locus_tag="VGA21_04140"
     CDS             complement(7366..9954)
                     /locus_tag="VGA21_04140"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HEX9649724.1"
                     /translation="MKNRNLVMIFVIVFLLSSFSLMAQKPDKKGNVYVDNSGVMRWEN
                     TREEVKGFGINYTVPFAHAYRSAGKLNVDREKAIDDDVYHFARLGFDLYRIHVWDTEI
                     SDTLGNLLDNEHLKLFDYLLFKLNERGINYVITPIAFWGNGWPEPDEKTPGFAYKYGK
                     GDCLTNADAIKAQENYLNQFLNHINSYTGVAYKEEPNMIAFEVSNEPHHREEPEKVTA
                     YIQKMVDAMRKTGCKKPIFYNISHSVHLAEAYFNADIQGGTFQWYPTGLGFQKELGGN
                     LLPNASNYEIPFDDVIKKNQGAKLVYEFDGADVGRAYIYPAIARSFREAGIQIATHFA
                     YDPTYLAFANTEYNTHYMNLNYTPSKALALMISGEIFHKIPMNKSYGLYPKNNSFDGF
                     RVSYEEDLAEYVTERKFIYTNTTSTMPANTSRLEQIAGTGNSPLVKYGGNGAYFLDEV
                     DKDVWRLEVMPDAVWVSNPFGRNSLKKVVGIIKWREWPMTLDIPALGENFTVQPLNGG
                     NEYRPEVNGKSFMVKPGTYLVARNGAKMNLTATDSWKNIKLGEFVAPESTVDRTYLLH
                     EPISETASGNEIQISAIAISNQVIKSVQLMVFAGFRPRILEMEETTPYTFTVTIPSDM
                     VKEGYVRYNLVITTESSSFTYPAGQEGRPFDWDFHDRSTYEVRVVNKSNSINIFNAIS
                     DNDYLARRWVRGIQQIPGIEPGVAEYQANIEKLHTPDNENVGAPEIHDYSFRYFFGDK
                     IKGRKDELDSAQKIIFRGRSLNDKPCTIQLALVLRDGSSWGGTIIVEPQRASYELSIS
                     DLRPVKTVTLPRPYPTFLPYYFEHEPSRFDISKAESLQFSIGPGIPESDRTVRHGIAI
                     ESVWIK"
     gene            complement(9962..12445)
                     /locus_tag="VGA21_04145"
     CDS             complement(9962..12445)
                     /locus_tag="VGA21_04145"
                     /inference="COORDINATES: protein motif:HMM:NF014850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HEX9649725.1"
                     /translation="MFCNVRRLFILFFLAISIPTFANNPEKRERYLMDFNWKFIQQDI
                     DGAQNPGFNDGDWRTLDLPHDWSVEGEFNRDAPGGGRVAYLPTGIGWYRKSFDLPVSA
                     AGNYVWIEFDGVYMNSTVWINGKELGMRPFGYIGFAYDLTPHLRPGKNTIAVRVDNSK
                     QPNSRWYSGSGIYRHVWLNITDNLRIDFLGTHIITSNITDDKADIIINTRLVNNHSSR
                     KSGILRTVIIDQTGKEVKRFDLGYSVNSGQSTMVKNKNTVPSPKLWGLKNPHLYTLHQ
                     MVMEGNRIVDDQVTVFGIRKIEYDSKRGFLLNGEQTKMKGVNLHHDGGAVGAAVPERV
                     WERRLIKMKEMGVNAIRTAHNPPAPEFLDLCDKLGFLVMDEPFDEWTHGKVEFGYNKY
                     YKEWSQRDLIDFIRRDRNHPSVVMWSAGNEIGEQSVDGGEEILKPLIETFHREDPTRP
                     VTTGNDHIAADGRTAKLPFLNMLDIVGYNYMDRWHERREIYNSEDKINNPNWKMVGTE
                     SASVRNIRSEYKVTDDIEATTYHYTSGMIRAAELWKFVSMHDYVIGDFMWTGIDYLGE
                     ARWPDKNASSGELDLAGFEKDGFYFYQSQWTEDPVLHVFPHWNWKGKEGRFLQVVAYT
                     NCDSVLLYLNDKLIGTRVLEFPRQGTSGGWNRYERPYLRTTTADLHASWDVPYEPGTL
                     RAVGKRAGKLVSIYEIKTTGEPAAIRLSADRQLIKSDKRDVVHVKVEIVDENGLVVPT
                     ASNEIEFSVDGVGKLIAVDNGDPSDHDSFQHNKRKAFNGLALAIIQSSDESGTIRIKA
                     TSPGLKDSNMEVKTDNSIQACFNYTKAIK"
     gene            complement(12597..14435)
                     /locus_tag="VGA21_04150"
     CDS             complement(12597..14435)
                     /locus_tag="VGA21_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015029741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HEX9649726.1"
                     /translation="MKKFISILCLFISTGTFSQVTRVEPPNWWVGMKDESVELLLYGK
                     EISDLTAEVEYKGVKITKVTPAESKNYLFLELSIAENTQAGEVSIKLNSGRRTVHTFQ
                     YQMQNRNQNPASLTGFNSSDVIYLITPDRFSNGNPDNDIVKGMRETSLNRQEDYARHG
                     GDLRGIIDHLDYLDEMGFTAIWSSPVLENNMKQQSYHGYAITDFYKVDPRFGTLDEYK
                     ELATKAKARGIKLIFDGVVNHSGSGYWWMEDLPFSDWINYQDSMRVTNHRRTVNEDLY
                     AAQVDRELMTRGWFVPSMPDLNQTNPHMAKYLIQNSIWWIETLGLGGIRQDTYPYSDQ
                     SFLRDWTCSIMNEYPNFSIVGEEWSTNPLIVAYWQQGSAIKGRVQGCLNSTMDFPMQS
                     NLVQALNEKESWGNGLVKLYDGLANDFIYANPQDLLVFGDNHDMDRIFTQLNEDVELT
                     KMALTYLLTVRGIPQIYYGTEILMQNSAKRGDHGLIRSDFPGGWPGDDINGFTATGLN
                     DHQKNTQEYLKALLNWRKDKKVIQTGKTLHFAPSDGVYVYFRYNESDIVMVILNKGQA
                     SRLNLLRFTEILGDKKNAINPFTGIYTDISGSIQIPAKSTLILEIK"
     gene            14579..18610
                     /locus_tag="VGA21_04155"
     CDS             14579..18610
                     /locus_tag="VGA21_04155"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HEX9649727.1"
                     /translation="MILRLAFLSILISGICNEIMAQSSVLFNSIDQTNGLSNGSVTKI
                     VQDAKGFIWIGTKNGIDRYDGLKFRIYDTYNSGLGANDISNIFIDDKQRMWVSTIGGG
                     LSIYDQAKDEFYTFQFDQGNPKSISSSDVQVIEQDREGNVWIGTEHGLNKFMEADSSF
                     EKFKYSERNSLFSNRVMAIFEDESENLWVGTFGGGLSLLNKQSGKLEIKHPIGNRSLE
                     LKFIHQINSLEPGKLLIATSGTGLIEYDIEKNFISSFLPEPFNRIPIVRSVYHGSGNT
                     LWVATDGEGLLKVTFQDGKKVIKQFINKTGNPNTLNSNALYTVFEDNSRNIWIGTAWN
                     GLNVLGNESENLNFYWSDFKGESQKGILSIYADDWKLYFGTDGSGLSTYDLISKGNAI
                     NGNFDHYYIQFIEKDKDGSLWIGTFKNGLIHLNNGEVEQFKHDAENKFSISHNDVRDI
                     LQLSNNKYLVATWGGGLDLFNSLEKTFTSNKNSQSEPGSIGSDNVISLLAADDGKVWV
                     ATFGGGLNLFDPVTETFEKIRIHIKDEAQLNGNNMLTLLKDSNNDLWLGAWGDGLIKW
                     NQNSKDFTRFTTKEGIADNTITALLEDQNGRVWMSTKNGISSLNINGNEINSYPLLKG
                     EYHINSSFAAKNGQLFFGNTKGAISFNPDRLAAGNQNPEIVFTELRIFDKQIQATEGV
                     IESQILFQDRIVLSHNQNAITFEFAALNYPFSNQFEYSYFLEGITSDWVNLGNQRSIT
                     LTNLMPGNYTLMVGGANMPKSYRNFASIDIEILNPWWKEWWAIATYFILFLILLYGFR
                     HYAVKWEKMKHSLKLEKISREKEAELHDIKMRFFTNISHEIRTPITLMLGAINRMEEK
                     GVLENLQKKALVSLRRNGNRLSQLLTELLDFRKLETAGFHLKISQGNLVKFAREVSLS
                     FNDIALQKKIDFQFQADAERIEAWYDKDQMEKVLFNLLHNAFKYANKNGEIRLKVERD
                     DENIFIRILNTGFPIPSEELRDVFKRFYQSDNTLSSKKTGTGLGLAIAKEIVHLHSGE
                     LLVESGKRVTTFTVKLMFGNDHFSPAELIEKSNSEDISNFYLEESEIQPADIRIKDEE
                     LFAGKRVLVVEDNHEVRKYIVEILQTDFTVMEAANGIEALDALHSEMPDLIICDVVMP
                     EMDGITFTSKIKSNRKTSHIPVIILTARTSLIYKKEGYETGADEYITKPFSESLLRTR
                     IRSIIRNRELIKMKLQNEFITEPKELAISTPDQQFLEDMMKIIEQNIDSQELKAEFIC
                     RELAMSHSVVYKKVKALTGLSLIEFIRDFKLKRAAQLMAKYQFSIVDACHKVGFSDRK
                     YFTQIFKSKFGMTPSEYIKVNLN"
     gene            18791..>19346
                     /locus_tag="VGA21_04160"
     CDS             18791..>19346
                     /locus_tag="VGA21_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015029746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015926 - glucosidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HEX9649728.1"
                     /translation="MKLTGITLLFLTAGILFLTCGNQDMPIEFKPEIPEEYYLGADLS
                     YVNEMLDCGGVYRENGNEVDPYALFAGKGASLVRVRFWNNPDWTQYSDFGDVYLTILR
                     SKAQGMAVLLDYHLSDTWADPAKQIIPKAWSEIDDMEILGDSVYNFISSTLRVLGNAN
                     LLPEFVQVGNETNNEILQPGEYTDY"
BASE COUNT         5579 a         3937 c         3915 g         5915 t
ORIGIN      
        1 aatcccaatg gaattgcgat gccaaacaat gcaatgagaa ccggaagaaa aagcgctgaa
       61 agtgctccgc ctgctccgaa gaatgtccac cacaagggat ccgatgtatt tagtcttttg
      121 ctcatttttc aacttatttt atcaccagcc agataatcaa aatcgaaatg aaaacccacc
      181 cggcataatt ggaaagcgcg actaccactc ccggtactct ttgtttccca atttttacaa
      241 ccattgcctt tggggcaaga ttaaaccagg tcacactatg gaatatcaaa atcaggaaag
      301 cgataatatg aaaagcaata aatccacctg actcaagttt ttctaaaaat gcgtcatatt
      361 ctgtggggcc tgaacttacc gcattaataa aaaacaaaaa taagattgaa taaaatgcaa
      421 caaacatgct ggtcagttcc ctggaaataa atttaatatg tacccatcgg tgcgtccacc
      481 agaaaattgg cattttcttt ttgtaggttt tgcctaaatt ttcagcttcc ataagccaat
      541 tattttttgc tccagggcaa taaaaacgat tgccaaaaat ttgcagctcc agccaactta
      601 tatctttgta tggcgcctgc tggatcaaca tcctttgggc atactgcaga acattctccc
      661 acgaaagtac attgccacaa cccttcctca gagttcaata cttccattcg ttcttttgat
      721 cctgaatcac ggttgtccat gttgtacctc tgggcaagtg caatagcagc cggtccaata
      781 aattcaggtt ccaggccata aaccggacaa gctgaataac aaagcataca gttaatacac
      841 atactgtatt gcttgtagtc atccagctga tcgggggttt gtaaatactc ttgctttagt
      901 tcggtatctt ccttttcacg gatgatccag ggtttaaccg ccttcaactt atccatgaaa
      961 tcagtcattt caataatcaa atcccttacc acaggaaagt ttgttaacgg ttcaattcga
     1021 atcggacccg gagcaaattt ttctataaaa gtggcgcagg ttagaacagg agtgccgtta
     1081 acaaccatgc cacaacttcc gcaaacgccc atccggcaag accacctaaa gcttagcgtt
     1141 ccatcaattt gatcttttat ataattcaag gcgtcaagaa taacccaatc cttcttcagg
     1201 ggaacttcat aattttgaaa tgcgggctcg ggatcttctt cgggacggta tcgaaaaact
     1261 tctaacgtta tggtatcttt cataatcaat taattcgttt ttgtttctct gccataaact
     1321 ctttccgccg gcggccatcg cgtaatagta acaggtgcat attgaatatc cggcggtgca
     1381 ttttctttcc tgaaagccag ggagtgagca aggaaatttt tatcatctcg ggcagcataa
     1441 tcagttctct ggtgtgatcc cctggactcc tttcgatgca gggcggattg aatcacaact
     1501 tcagcgatat caagcagatg ggacagctca atggcggacg tgagttctgt attaaaagta
     1561 gaactgcgat cggagataat aatatttttg aatctttcct gcaactccat aactatcgcc
     1621 tgcgatcctt ttagcgaatc ttctgtgcga taaattccgg cacccgattc catacaaaca
     1681 tgcatctcct ttcgaaggtt ggcgatcttt tccgatccct ctttcttttc caaataattt
     1741 tccttgaggc gcttcacttc atcggttgcc agggagggta acgctgaact aaatcttcct
     1801 ttagtcgtag caaaattagc agcattttgg ccggcccttg ccccgaaaac aagcaattca
     1861 gttagggaat tagaccctaa cctgtttgct ccgtttatgc taacgcacgc ggtttcgccg
     1921 gccgcgtaca aaccctccag tttagtggca ccctcaatat tagtatcaat acctcccatc
     1981 atgtaatgga caaccggtct tacaggaatc atttccttta ccggatctat attttcgtat
     2041 ttcaggcata attctcttac aaacggaatt ttgctgttta tgatcttttc tccaaggtgt
     2101 cttatatcaa gatgaacgta atgcccgtat ggtcctgcga gtgttcttcc tttctcctgc
     2161 tcttttacaa aagcctgact caacctgtcg cgcggaccta attccatagt tcgcagaacc
     2221 ggttttggct gcggagtacc caaatcataa tcttgcaggt aacgataccc gtctttatta
     2281 atcatgtacc cgccctcgga tctggctgct tcggtaataa gaattccggt gaatggcaaa
     2341 cctgtagggt gatattgaac aaattccata tcttttaatg gaacaccggc ccggtaagca
     2401 agtgccatcc catccccggt tttaattgca gcattggtgg tgaatgggaa aacttttcca
     2461 catccgcctg tgcaaacaat tacagcgcgg gctgcgatat aatggatctt acctgttgcc
     2521 aattctatgg ccattgcccc ctggcaacga ccttcatcca ccagcagatt tgtcacaaac
     2581 cattcatcat atctttgcag tttattgtac ttcagagtgg tttggaagag cgtgtgaagc
     2641 atatgaaacc cggttttatc ggcggcaaac cacgtgcgtt cagttttcat cccaccaaaa
     2701 ggcctgacag ccacagaacc atcgtccatc ctggaccatg ggcagcccca atgctccagc
     2761 cggatcatct cttttggggc ttcattaaca aatgcctcca ctgcatcctg gtcactcatc
     2821 caatcgccgc cagaaatagt gtcgtaggca tgaaggtcgg gactgtcggt agatttgatt
     2881 accgccgctg ctccaccttc agcagaaaca gtatggctgc gcatgggata aactttggaa
     2941 attattccaa cggagagatt gggattttct tcggcggcgg caatcgcggc acgtaatccc
     3001 gcgccaccgg ctccaatgat taggatatca cattcgtgac gggttaattc catatcttga
     3061 tgaaataaag attcattccc gccctataat cattcataaa atttattgga aggtgataaa
     3121 ttcttccacc aatattttag ccctttcaaa tccaaatact aatcaatagc gacgggttta
     3181 gttcaaaaca agcaatgagt ttaaagaaaa atatgggcaa aatcaaaccg ctcatccagt
     3241 aaaagttatg aacggctgaa attcaattag ttgtggtgaa gcagggttgc gaaaaggagg
     3301 tgagagtttc taaagtcacg tctcaaaaga ttatttatta tgaagatttt gttcaattga
     3361 attttgatga tgcctggcgt ggtgaatagt aaaatacatc atctccctaa gggtgacctt
     3421 tcccaataat gggtgaggaa ggatcaaagt atccaatctt tcttcggaat acctttctaa
     3481 ttttttgcaa agtttatcaa cattagttct tatttgccgg gtaattattc gctgactttc
     3541 aaccggaact tcttttggaa tatagggact ggatgcagtg cctccctggc tgagtttgta
     3601 tttgtatttt tcaaccaatt cctcgtaggt tttcgatgga cgatttgctt tgccgacata
     3661 tattttgaga aaccaatccg gaagtgaaag agccatgttg agaggtttaa tactcattaa
     3721 caaatgtttt gcctgctgtc ctgaagtcca cttgtccgga gcgggggaga aaagaaattg
     3781 cttttcagtt aaagaagaaa caaaagcggt gaagctttta taagaatcct gcaactgccc
     3841 aataatttct tcccgggtca tttaaaataa aaatagggag aaagaactaa caatcagctg
     3901 ttccttagac aaaatattta tttcgaacct ttttcttata tttctaaatt acttttttga
     3961 aatggacaaa gacagaggat ttaaacgagc tataaaactt cctcatgcaa cagccctggt
     4021 tgccgggacc atcatcggtg cttccatatt tgtacaacct tcagaaatta ccggacaggt
     4081 tcattcagtc gggggagttt tcattgtctg gattgttgca ggtattctta cattttttgg
     4141 agctttggtt tgcgcggagc tttcatccat tttcactaaa accggaggtg tttataccta
     4201 cctgaaggaa gcatttaatc cggccatggg attcctgtgg ggttgggcaa tgttctggag
     4261 catgcacacc ggaattatcg ccgcggtcgc ggtaattttt gcaagatata ccggttattt
     4321 ttttcctttg aatgacctgg aaataaaact tgtggcaata atcttaattc tatttcattc
     4381 tgcagtcaat tattttggag ttaagcatgg cagcatgctt cagactctgg ttacagcagg
     4441 caaagttctg gttattggga ttattatcct ggcaggattt gccattggct ccaaattacc
     4501 taatcatttt gaagcaggat cttttgaaag catgtcaacc tcacccggag atattctgtc
     4561 ggcattaatt gccggacttt ttgcatttgg tggatggcac atggttacct ataattccga
     4621 agaaacagtg gatgccaaaa ggacgattcc aaaagcactt ttatggggaa ctttgattgt
     4681 tacagtctgt tatcttgcat tgaatacagt atatctatat gtattacctc tggataaggt
     4741 tgcttcttca acaaaaattg ccgcggacac agcggattac ctgatggggt cgggtggtgg
     4801 aatgtttatg gctggcctgg ttatgttttc aacatttgga tcgatctccg gaatcattct
     4861 aacgggtccg cgggtttatt attcaatggc acaagacgga cttttattta agtgggtcgg
     4921 agttttacac tcaagatatg gtactccgca cagggctatt tttttgcagg ctatctgggc
     4981 atccattctg gtatttaccg gaacttacag ggaattattt accagagtaa tttacacaga
     5041 atggatcttc tttggcctga tggctattgg cctgattctt cttcgaaaaa gaaaagacat
     5101 aagccgcggt tattcaattt ttggttaccc ctggatcccg gtaatattta ttatttgctg
     5161 cttcaccatt gtttttaatc atattttgaa tgaaccggtc gaaagtctta ccggactttc
     5221 ttacgtactt gcgggtttag ttgtatattt ctttttgaag tttaataaga aatctatcac
     5281 atgatcattg actttcacaa tcatttttat cctccggctt atattgatgc aataaagaaa
     5341 aattcatctt tctataaagt tacctatgac gaagacgaga atccggtcat ccatagtccc
     5401 ggggattaca atattgtagt tccgggccac cggttggtag aagtacgatc caaggtattg
     5461 aaagacgctg aggttgatat gcaagtactt tcttttacta ctcccggcac catagtggaa
     5521 ccggctgaaa gatctgttga gctggcaaga atggtaaatg acacctttgg tgaaattatt
     5581 aaaacaaatg ccgatcaatt tactgctttg gctacattac cgctaaatgc tccggaagca
     5641 tgtgccggtg aaatggaaag agcaattaat gaggttgggc taaaaggcgt tatgctctat
     5701 tccaacgcca atggtgttgc actggctgat aaacgcttct ggcctatgta tgaaaaggcc
     5761 aatgaaatgg aagcagtatt gtacatacat cctacgtatc cggttggagt tgaggccatg
     5821 gaagactaca tgctgatgcc cctgatcggt ttttgttttg acactacttt ggctgcagcc
     5881 aaacttgttt ttgaaggcat tccaaggcgc tatcccaaaa ttaaatgggt acttggacat
     5941 cttggtggtg caattcctta tctggctgaa cgacttgaca gaggatatga agcttttccc
     6001 gaatgccggg tgaacatcga tcagaaaccc agtgaatatt taaagaaatt ctattttgat
     6061 acggtaaatt ttgatttgaa tgccttaaat cttgctgttg agtttgccgg agtcgatcag
     6121 attttagcag gcagtgacta cccgcatcaa attggaagtt tgaagaaaat gaaagaaagt
     6181 ttgaagaatt taaatatttc gaaatctgaa atggaaatga ttcagagtga aaataccaaa
     6241 aaacttttgg gtctctaagg cttgggggta gcgtagatat ttattgtttg cttatataaa
     6301 gtgatgtcac ttttgctttt caatggaaca attttagcgt ccatcacaaa atcactgaag
     6361 taataaatat cataacccaa atcttcaatc accgaatata gttcaatttt ttctgcatcc
     6421 gaattctttg taaagctttc ggcaacaaga gtgggtttgt actttgctaa taaatctgaa
     6481 attgatttaa ttatttcctt atcgtaaccc tctgtatcaa ttttaataaa tgacagctta
     6541 ctcatccagt ccggatactc tttctccaaa aagtcactaa gtttaatacc gataatcttt
     6601 tcgggataaa taaattttcc atgaatgctg tctttagtag gagaaattcc accatttgca
     6661 aaagaagcct ctgacgaaat aaaataaaac tcagcttctt cttttgagat ggcataagga
     6721 agtggaacta ggttatactt tccataatta agacttgaat tcttttcaag aatcttgaaa
     6781 acatatggat taggatcaaa tcccagagtt aatccaccaa cacctgcaga caagcccatt
     6841 ggtaccgtag tgtctccaaa attggcacca atatcaatta ccagatcacc ttctttaatg
     6901 aattttgaaa agaaatcaac attagattgt gtaatcacaa attccggtgc caatgggtta
     6961 tcccaattag caaattctat tgggcccaaa ttttccaggt gataggtttt gacaatcggc
     7021 gggtattcct gggttacccg ccttgctttc ttcctcctga gggattgcct aatgtaattt
     7081 agcatgctaa attgtctcaa ctaattaact tgtgaagata aatgaaattg aattaattgt
     7141 gagatcattt ttatcaataa atactactaa aataaataga tttgattatg ataggtgatt
     7201 cagggaactt ttgttaatct actttggtaa tctttttttc cgatgcagtt tttcaataaa
     7261 tttccttact gattgaattt atgagaggag tattattctt cattttgatt ataacagaag
     7321 tatcttttca agggcaggtc tgcttcggat tccaaggggg tccggtcatt taatccatac
     7381 gctttcaata gcaatcccat gtcggacggt tcgatcactt tctggaattc cagggccaat
     7441 ggagaactgc aaactttctg ctttgctgat atcaaacctg gatggttcat gctcaaaata
     7501 atacggcaag aaagttggat agggcctggg aagagttact gtctttacag gtctcaagtc
     7561 actaatggat aattcgtaac tagccctttg cggttcaact ataattgtgc caccccaaga
     7621 cgaaccatcc cttaaaacta aagccaattg gatagtacaa ggtttatcat tcagggatct
     7681 gcctctgaag attatttttt gagctgagtc caattcatct ttccttcctt taattttatc
     7741 accgaaaaaa taccggaaac tatagtcatg aatttccggg gcgccaacat tttcattgtc
     7801 cggagtatgt aatttttcaa tgttcgcctg gtattcggcg acacccggtt ctatacccgg
     7861 aatttgttga attcctctta cccatcgtct ggccaggtaa tcattatcag aaatagcatt
     7921 gaaaatgtta atagaattcg atttattcac aacccgaacc tcgtaagtac ttcgatcatg
     7981 aaaatcccaa tcaaaaggcc ttccttcctg acctgccggg taagtgaatg atgatgactc
     8041 ggtggttatt accagattat agcgaacata accttcttta accatatcgg atggaattgt
     8101 tacagtgaaa gtataaggag tagtttcctc catttcaagg attctgggtc gaaatccggc
     8161 aaataccatt agctgaaccg atttaattac ttgattagaa atagcaattg cagagatctg
     8221 aatttcattt cctgacgcag tttcggagat gggttcatgc agtaagtaag tcctgtcaac
     8281 ggttgattcc ggtgctacaa attctcctaa tttgatattc ttccaggaat ctgttgcagt
     8341 gagattcatt ttagctccat tccttgcgac cagatacgtt ccgggtttca ccatgaaact
     8401 tttgccgttc acttcagggc ggtactcatt cccgccattt agcggttgga cggtaaaatt
     8461 ttcaccgagg gcaggaatat ccagggtcat tggccattca cgccatttga ttatgccaac
     8521 aactttcttc aggctgtttc ttccaaaggg attacttacc caaactgcat ctggcattac
     8581 ctcaagccgc caaacatcct tatccacctc atcaagaaaa tatgcgccat ttccaccgta
     8641 tttcacaagg ggcgaattgc cggtccccgc gatttgttcg agcctggaag tgttggcagg
     8701 catagttgat gtggtatttg tgtagatgaa ttttctttct gttacatact cggcaagatc
     8761 ctcttcataa ctgaccctga acccatcaaa agagttattt ttcggatata atccatagct
     8821 tttgttcatt ggtatcttat gaaagatttc cccggaaatc attaaagcca gcgcctttga
     8881 aggtgtataa ttcaaattca tataatgcgt attatactca gtgtttgcaa aagccaggta
     8941 agtgggatcg taggcaaagt gtgttgcgat ctggatgccg gcttccctga aacttcttgc
     9001 tattgctggg tatatatacg ctctgcctac gtcagctcca tcgaactcat aaaccagttt
     9061 tgcaccctgg tttttcttta taacatcatc aaatggaatt tcataattgc tggcatttgg
     9121 caggagattg ccacccaact ccttttgaaa tcctaacccg gttgggtacc attgaaaggt
     9181 tccgccttgt atatcagcat tgaaataggc ttctgccaga tgaacgctgt ggctgatatt
     9241 ataaaaaatc ggtttcttgc acccggtttt tcgcatagca tcaaccattt tttgaatata
     9301 agctgtgact ttttccggct cttcacgatg gtgtggttcg ttgctaactt caaatgcaat
     9361 catgttaggt tcctctttat acgccactcc tgtgtatgaa ttaatatgat tcaaaaactg
     9421 attaagataa ttctcctggg cttttatggc atccgcgttg gtaagacaat cgccctttcc
     9481 atatttgtac gcaaatccgg gagttttctc atcgggctcc ggccagccat ttccccaaaa
     9541 agcgatgggt gttattacgt agttaatccc tcgttcattt aatttaaaca agagatagtc
     9601 aaacaattta aggtgttcat tatccaaaag gttgccgagg gtatcgctaa tttcagtatc
     9661 ccaaacatga attcggtata aatcaaatcc gagccttgca aaatggtaca catcatcgtc
     9721 aattgccttt tctctgtcaa cattgagttt acccgcagat ctgtaggcat gagcaaaagg
     9781 cacggtataa ttgattccaa agccttttac ttcttccctg gtattttccc atcgcataac
     9841 cccactatta tcaacataga catttccttt tttatctggt ttttgagcca ttaaagaaaa
     9901 actactaagt aaaaatacga ttacaaaaat cattaccagg tttctatttt tcacaagatt
     9961 gttatttaat tgctttggta taattaaagc atgcctgaat tgaattgtcg gttttcactt
    10021 ccatattgga atcttttagc cccggagagg ttgctttaat cctaatcgtt cctgattcat
    10081 ctgaagattg aataatggca agagcaagac cgttaaatgc cttgcgttta ttatgctgga
    10141 atgaatcatg gtcggatggg tcgccattat caacggcaat aagtttgccg acaccatcca
    10201 ctgaaaattc aatctcattg gatgcggttg gaactaccaa tccgttttca tcgacaattt
    10261 caaccttaac atgaacgaca tcgcgtttat cagattttat cagttgacga tcagcggata
    10321 accggatagc ggcaggctcc ccggtagttt tgatctcgta aatagaaacc agcttgcccg
    10381 ccctttttcc aactgcgcgt aaagtgccgg gttcataggg aacatcccag gaagcatgaa
    10441 ggtcggcagt agtagttctc aggtaaggtc tttcataccg gttccaacca ccggaggttc
    10501 cttgtcttgg aaattccaaa accctggtac caatgagttt gtcattcagg tataacaaaa
    10561 cactgtcaca gtttgtatat gccactacct gcaggaaccg gccttccttg cctttccagt
    10621 tccagtgtgg aaatacgtgt aatacgggat cttcagtcca ttggctctgg tagaagtaaa
    10681 acccatcttt ttcaaacccc gctaagtcaa gttcaccgga gcttgcattt ttgtcaggcc
    10741 atctggcttc gcccaggtaa tcaatacctg tccacataaa atcgccaata acgtaatcat
    10801 gcattgagac aaatttccat aattctgctg cccggatcat cccggaggta taatggtaag
    10861 tggtggcttc aatatcatct gtcactttgt attcactacg gatatttctg acagatgcgc
    10921 tttccgtgcc caccattttc caatttgggt tattaatttt atcctcgctg ttataaattt
    10981 cccggcgctc atgccagcga tccatataat tgtagcccac gatatccagc atattcagaa
    11041 atggtaattt tgctgtgcgg ccatccgcgg caatatgatc gtttcccgtt gttacgggcc
    11101 gcgttgggtc ttccctgtga aatgtttcaa tcaatggctt caggatttct tcgccaccat
    11161 caacactttg ttcaccaatt tcatttcctg cactccacat tacaactgag ggatgatttc
    11221 tgtctcttcg tataaaatct attaggtcgc gttgagacca ctctttatag tatttgttat
    11281 acccaaactc aacttttccg tgtgtccatt cgtcaaaagg ttcatccatt accaggaagc
    11341 caagtttatc acaaaggtca aggaattcag gtgcgggagg attatgcgcg gtgcgtattg
    11401 cattgactcc catctctttc atttttatta gtcttcgttc ccaaactctt tctgggaccg
    11461 cggcaccaac tgccccgcca tcatgatgaa gattaactcc cttcattttt gtttgctctc
    11521 cgttcaacag aaatcctctt tttgaatcat actctatttt cctgatcccg aaaacagtaa
    11581 cctgatcatc aacaatccta tttccttcca taaccatttg atgcaaggta tacaagtgtg
    11641 gatttttcag tccccagagt ttaggagatg gaacggtgtt tttatttttt accatggtgg
    11701 actgccctga atttacagaa taaccgagat caaatctttt aacttctttg ccggtttgat
    11761 ctataattac tgttcttaga attccgcttt tacgggaaga atgattatta accagacgag
    11821 tattaataat tatatcagcc ttatcatcag taatatttga agtaataata tgagtaccca
    11881 ggaagtcaat gcgcaggtta tctgtgatgt taagccagac gtggcggtaa atacctgacc
    11941 cggaatacca tcgcgaattg ggttgtttcg aattatctac acgaaccgca atcgtatttt
    12001 tacctgggcg caagtggggt gtcagatcgt atgcaaatcc aatgtatcca aaaggacgca
    12061 ttcctaattc ttttccattg atccaaaccg tactattcat atacacgccg tcgaattcaa
    12121 tccagacata gttgccggcg gcactgacag gcagatcaaa actctttcgg taccagccaa
    12181 ttccggttgg gaggtaagca actctgccgc ctccgggagc atctctgtta aattcacctt
    12241 caacgctcca gtcgtggggt aaatccaggg ttcgccagtc gccgtcatta aaccccggat
    12301 tctgggcacc atcaatatcc tgttgaatga atttccagtt aaaatccatc aggtagcgct
    12361 cccttttctc cggattgttg gcgaaagttg ggatggaaat agcaagaaaa aatagtatga
    12421 ataaccttct gacattgcaa aacataatgg agttgtttta aggtcaaaat aatagcagtg
    12481 agcctggaaa ttcaaataaa cagtagccct ggaaggctgt gatttcatct aattgaaggg
    12541 gtattttaga tttgcttttg atctcaaata attgctatca ggagcatccg agcggattat
    12601 tttatctcca atatcagggt tgattttgcg ggaatttgaa tggaaccgga tatgtctgta
    12661 tatatgcctg tgaatgggtt tattgcattc tttttatctc ccaatatctc agtgaatcgc
    12721 agcaaattca gtctggacgc ttgtccctta ttcaaaatta ccatcacaat atctgactcg
    12781 ttgtatctga aatagacata tacgccatca cttggtgcaa aatgaagggt tttgccagtt
    12841 tgtattacct tcttgtcttt tctccaattg agcaaagctt tgagatactc ctgtgtattc
    12901 ttctggtgat cattcagccc tgtcgcagtg aaaccattaa tatcatctcc aggccatcct
    12961 cccgggaaat cagatcgaat caaaccgtga tcaccacgct tggctgaatt ctgcatcaga
    13021 atttccgtac catagtaaat ttggggaatt ccccggacgg ttagtaaata agtcaatgcc
    13081 attttggtga gttcaacatc ttcattaagc tgggtaaaaa tacgatccat atcatgattg
    13141 tctccgaaaa ccaacaagtc ctgaggattc gcataaatga aatcattggc caatccatcg
    13201 tatagtttta ccagcccatt tccccagctt tctttttcat tcaatgcctg aacaaggttc
    13261 gattgcatcg ggaaatccat ggtgctattc aaacaaccct gtacccggcc ctttattgct
    13321 gagccttgct gccaataggc aacaatcagc ggatttgttg accattcttc acctacaata
    13381 ctaaagtttg gatattcatt cattatggag catgtccagt cacgaagaaa cgattgatct
    13441 gaatagggat atgtgtcttg ccggatccca cccagtccca aagtttcgat ccaccaaatg
    13501 ctattttgaa taagatattt agccatgtgc gggttagtct ggtttaaatc cggcattgaa
    13561 ggaacaaacc agccccttgt cataagctcc ctgtctacct gggccgcata aagatcttcg
    13621 ttgacggttc ttctgtgatt ggtcacacgc atggagtcct gataattaat ccaatcagaa
    13681 aatggcaaat cttccatcca ccagtaaccg gaaccggagt gatttacaac tccatcaaaa
    13741 attaatttaa ttccgcgggc ttttgctttg gtggcaagct ctttatattc atccaaagtg
    13801 ccgaatcttg gatcaacttt gtagaaatcg gtgatggcat atccatggta agactgctgt
    13861 ttcatattgt tctccagaac cgggcttgac catattgcag taaatcccat ttcatccaga
    13921 tagtcaagat gatcgatgat accgcggagg tcacctccat gtcttgcata gtcttcctgc
    13981 cggttaaggg aagtttccct catccctttt acaatatcat tatcaggatt gccgttcgaa
    14041 aaacggtccg gagtgataag gtatatcaca tcagaagaat taaatccggt aagagaagca
    14101 ggattttgat tgcggttttg catctggtat tggaatgtat gaactgtcct tctcccgctg
    14161 tttagtttta tagaaacttc accggcttgc gtattttctg caatggaaag ttccaggaaa
    14221 agataattct tgctttctgc cggcgttact ttcgttattt ttaccccttt gtattcaacc
    14281 tccgcagtaa ggtcacttat ttctttacca tagagcaaca attcaacgct ttcatctttc
    14341 attcctaccc accaatttgg cggctcaaca cgtgtaactt gtgagaaagt ccctgtcgaa
    14401 ataaatagac ataatattga aatgaatttc ttcataataa ttattcagca ttaatgattt
    14461 gatgttatac cttcataaat atccggaaaa tagtcttttt agaagagcca ttcatataaa
    14521 gataagtttt aaaaaagtgt tttatcacgt aaattatggg taataaattc ccatttccat
    14581 gattctgaga cttgcgttcc tttcaatcct tatttcaggc atttgtaatg aaattatggc
    14641 tcagagctcg gtacttttta attctataga tcagacaaac gggctttcta atggttcggt
    14701 aaccaaaatc gttcaggatg ctaaaggatt tatttggatt ggaaccaaaa atggaatcga
    14761 ccggtatgat ggattaaaat tcaggatata tgatacgtac aactccggtc tgggtgcaaa
    14821 tgacatcagc aatattttta ttgatgataa acaacggatg tgggtaagta caattggtgg
    14881 tgggcttagc atttatgatc aggctaaaga tgaattctat acttttcaat ttgatcaggg
    14941 taacccaaag tcgatttctt caagtgatgt ccaggtaatt gaacaggatc gggaagggaa
    15001 tgtttggatt ggtacagagc atggtttgaa taagttcatg gaggctgatt catcttttga
    15061 gaaattcaaa tacagcgaaa ggaattcact cttcagtaac cgggtgatgg caatttttga
    15121 ggatgaaagt gaaaatttgt gggttggaac gtttggtggg ggtctcagtc tgttaaataa
    15181 acaatcgggt aagttagaga taaagcatcc catcggaaat cgatctcttg aactgaaatt
    15241 tattcatcaa ataaattccc ttgaaccggg caaacttcta atagccacaa gtggaaccgg
    15301 tttgattgag tatgatattg aaaaaaattt catttcaagt tttcttcccg aaccttttaa
    15361 taggattcct attgtgaggt ctgtctacca cggtagtggg aatactcttt gggtggcaac
    15421 tgatggagaa ggtttattga aagttacttt ccaggatggc aaaaaagtga tcaaacaatt
    15481 cattaataaa accggcaatc ccaacaccct gaatagtaac gcgctgtata cggtttttga
    15541 agataattcc cggaatattt ggataggcac tgcctggaac ggactaaatg ttttgggcaa
    15601 cgaatccgaa aatctgaatt tttattggag tgactttaaa ggagagagtc aaaaaggcat
    15661 cttaagtatt tatgctgatg attggaagtt atatttcggc acagatggat ctggcctgag
    15721 tacctatgac ctgatttcca aagggaacgc gattaatgga aactttgatc attattatat
    15781 tcaattcatt gaaaaggaca aagacggatc cctttggatt ggcaccttta agaatggatt
    15841 gattcacctc aacaatggag aggttgagca atttaaacat gatgcggaaa ataaattttc
    15901 aataagtcac aacgatgtaa gggacatact tcaattgagt aataataaat acctggtggc
    15961 tacctggggc ggtggtctcg atttgtttaa ttcgctggaa aaaaccttta catccaataa
    16021 aaatagccaa tctgaacccg gatcaattgg cagtgataac gttatttcac ttttggccgc
    16081 ggatgatggt aaagtgtggg tggccacatt tggaggtggg ctaaacttat ttgatcctgt
    16141 cacggaaact tttgagaaga tcaggatcca tataaaagat gaggcccaat tgaatggcaa
    16201 caatatgctc actttgctga aagactctaa taatgatttg tggcttggag cctggggaga
    16261 tggtttgata aagtggaatc agaattccaa agatttcacc aggtttacta caaaagaagg
    16321 gattgctgat aacactataa cggcgctatt ggaagatcaa aacggaaggg tttggatgag
    16381 cacaaagaat ggcatttcca gtttgaatat taatggcaat gaaatcaact cgtatccttt
    16441 actgaaaggt gaatatcata tcaattcttc cttcgcggcc aagaatggac aattattttt
    16501 tgggaatacc aaaggggcga tttcatttaa tccggacagg ttagctgccg gaaatcaaaa
    16561 ccctgagatt gtctttacag aattaaggat tttcgataaa caaattcaag caacagaagg
    16621 ggtaattgaa agccaaatac tgtttcagga tagaatagtt ttatctcata atcaaaatgc
    16681 aattactttt gaatttgcag cacttaatta tcccttttcg aatcagtttg aatactcgta
    16741 tttcctggaa ggtattacca gcgattgggt aaatcttggt aaccagaggt caattaccct
    16801 gacaaatctt atgcctggta attatacgct aatggtaggc ggagcaaata tgccaaaatc
    16861 ttaccgaaat tttgcaagca ttgatattga aattttgaac ccgtggtgga aagaatggtg
    16921 ggcaatagcg acttacttta ttttgttcct gattcttttg tatgggttca gacattatgc
    16981 agtgaagtgg gaaaaaatga aacacagttt gaagttggag aaaatttcaa gggagaaaga
    17041 ggcggaactt catgacatca aaatgcggtt tttcaccaac atttcccatg agattcggac
    17101 tcctattacc ctaatgctag gagcaataaa caggatggaa gaaaaaggcg tactggaaaa
    17161 cctccagaag aaagcgttgg tcagtctaag gaggaatggc aaccgtcttt ctcagctgct
    17221 taccgagttg cttgatttcc ggaaactgga aaccgccgga tttcacctta aaataagcca
    17281 gggtaacctg gtgaaatttg cgcgggaagt atcattatcg ttcaatgata ttgcactaca
    17341 aaagaaaatt gactttcaat tccaggctga cgctgaaagg attgaagcct ggtatgacaa
    17401 agatcagatg gagaaagttt tattcaattt attgcataac gcgtttaaat atgcaaataa
    17461 gaatggagaa atcagattaa aagttgagcg ggatgatgag aatattttta tccggattct
    17521 aaataccgga tttccaattc catccgagga acttcgcgat gtgttcaaga ggttttacca
    17581 gagtgataat acactttcat caaaaaaaac cggaaccgga ttaggtcttg ccattgcaaa
    17641 ggaaatcgtt cacttgcact ctggcgaatt acttgttgaa agtggaaaac gcgtgaccac
    17701 ctttacagtg aagttaatgt ttggaaacga tcattttagc cctgccgagt tgattgagaa
    17761 atcaaacagc gaagatatat caaactttta tttggaagaa tcagaaattc aaccggctga
    17821 tatcaggatt aaagacgaag aattgtttgc cggtaaaagg gttcttgttg tggaggataa
    17881 tcacgaggtg agaaaatata ttgtcgaaat tctgcaaaca gacttcacgg taatggaagc
    17941 agccaatgga attgaagccc ttgatgcttt gcattccgaa atgcctgatt taataatttg
    18001 tgatgttgtg atgcctgaaa tggatggcat tacattcacc agcaagataa aatcaaacag
    18061 gaaaaccagt catatcccgg tgattattct tacagcccga acctccttga tttataagaa
    18121 ggaaggttat gaaaccggtg ccgatgagta tatcaccaaa cctttcagtg agtcattgtt
    18181 gcgaacaagg atccgtagta ttatccgtaa ccgggaatta ataaagatga agctgcagaa
    18241 tgagttcatc actgaaccaa aagagttggc tattagcact cctgaccagc aatttcttga
    18301 agatatgatg aagataatag aacagaacat tgatagccag gagttgaaag cagaatttat
    18361 ttgccgggaa ctggcaatga gtcattcggt ggtttacaag aaagtaaaag ctcttacggg
    18421 attatctttg attgaattta tcagggactt caagctgaag cgagcggccc agttgatggc
    18481 aaaatatcaa ttcagtattg tagatgcatg ccataaagtt gggttttcag atagaaagta
    18541 tttcacccaa atctttaaga gtaagtttgg tatgacacct tccgaatata ttaaggtgaa
    18601 tctaaattga tatattctaa acgaattcag cccccatttt agccataaag aaacaccaca
    18661 tcttttcttc aaaattgtaa ttttaaccac aaccgtgaaa aagcgaattt ctcttagact
    18721 tacctatatc agccttgtat taattctact gtttggctgg ctaatggcaa tactattaaa
    18781 tcttatataa atgaaattga caggaattac gctcctattt ttaactgctg gtattctttt
    18841 tctaacctgt ggcaaccagg atatgccaat tgaattcaaa ccggaaatac cggaggagta
    18901 ttatctgggt gcagaccttt cctacgttaa tgagatgctt gactgtggtg gagtttaccg
    18961 ggaaaatggt aatgaagttg atccctatgc tttgtttgcc ggcaaaggag caagcttagt
    19021 tcgggtaagg ttttggaata accctgactg gacgcaatac tctgattttg gtgatgtcta
    19081 tttaaccatt ttaagatcaa aggcacaggg aatggcggtg ctgctggatt atcacttatc
    19141 ggatacctgg gcggatccag ccaaacaaat aattcccaaa gcatggagcg aaattgacga
    19201 catggaaatc ctgggagatt ctgtttacaa tttcatttca tctacgttaa gagttttggg
    19261 taatgctaac ctgcttcctg aatttgttca ggtaggtaat gaaactaata atgaaatact
    19321 tcaacccggg gaatatactg attaca
//
LOCUS       DASWHX010000058        34737 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_43605, whole genome shotgun sequence.
ACCESSION   DASWHX010000058 DASWHX010000000
VERSION     DASWHX010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 34737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34737
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43605"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(127..1152)
                     /locus_tag="VGA21_04165"
     CDS             complement(127..1152)
                     /locus_tag="VGA21_04165"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9649729.1"
                     /translation="MKSYIPILSLLSLGLLCCSNPAERAQDLLKVGNEHLSRGELAKA
                     EDYYNQAITEFPEFPHALNNLGIIRFKQQRYYLALELYDKAIALKPDFPDAYLNRSAA
                     LYEIGLFESSLRDIEIVEEITPDTFHINFRKGLIYTQLKKYDSAINYFSLAITYRSDN
                     PELWTNRGTVYYYKNQLNRAISDLTKALNLDTTFSPALNSIALVYLRKGNLDSAEINI
                     KRALMLQPKDSYYLNNYGLIVFRKGDINSALKLINQSIVINPDNGWAYRNKALVYLEQ
                     KDYNRALQLFYEASIRGGSVESLFSFWADAFYRSGDLKSACKKLDEARERNEDFAFLA
                     WQSECIN"
     gene            1435..2490
                     /locus_tag="VGA21_04170"
     CDS             1435..2490
                     /locus_tag="VGA21_04170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HEX9649730.1"
                     /translation="MLLLGSIVALVVSFYLLAQVSDKYFIESLDKIAHRFNMSHDMAG
                     ATLMAIGSSAPELFVAIIALVKPGDHGEIGIGTIVGSAVFNILGIIGAVAVVRKSVLA
                     WQPVLRDLIFYSISIITLIVVFYDSQIELYEGAILIFIYGFYLLAVSKWRKLFPYEEL
                     DTIEEEEEGEGEEEAPVWEKVFRPFDYAIDKLFPPIRYYYGVFFISIIVIALLSWVLV
                     ESAVIISEMLNIPKVVIALTVLAIGTSVPDLMSSVIVAKQGRGGMALSNAVGSNIFDI
                     LICLGLPWLLIISFSKTPLPVKTDDLFVSVILLFSSVLIILALLVINNWSIGKKAGIF
                     LLLLYLAYVIREFYLAI"
     gene            complement(2537..5935)
                     /locus_tag="VGA21_04175"
     CDS             complement(2537..5935)
                     /locus_tag="VGA21_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009581850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX9649731.1"
                     /translation="MVKFVTRFTLCLIAVFLYQQAESNIFLEQDTVKSDSTKKEKKEK
                     KDLPLEVGRHIKFNTREGSWISVDISPDGSTIVFDLLGDLYTMPAAGGKATRISSGLA
                     FDSHPRYSPDGKKIVFISDRSGAENVWIAKLEPDTIKFEAISEGKNNSYQSADFSPDS
                     KYIVASKGGALLGVPKLWLFHVDGGSGTGLIEGPTNLKTVEPAFSPDGRFIYYSRRFG
                     SWNYNAQFPQYQIAIYDRETGESETITSRYGSGFRPTPSPDGKWLAYGTRHDDKTGLI
                     IRNLATGDEKWLAYPVQRDDQESIATMDVLPGMSFTNDNQFLICSYGGKLFKIPVNGG
                     SAVIIPMDIDVDLELGPELDFKYPISDSTEFIARQIRDPAVSPDGKKAAFTVLDKIYV
                     MDYPDGKPVKLTNSNDVEAMPAWSPDSKWIAYVTWSNEGGHIYKVTVSGKPSPVRLTQ
                     TAALYSTPAWAENNRIVYVRGAAQIYKESVDPFNSGTSVDLGWVSANGGPERFITKTR
                     GRLTPHFVKGQDRIYLYQGGTNGKGLVSIRWDGTDEKAHLKVTGITTFGAQNPSTADL
                     IMMAPEGDQAMAQVNNDVYVVTVPYVGGETPKVSVSDPDKAQFPSRKVTDIGGEFPTW
                     GSDGKKVYFSLGNAFFKYDITAAKVYEDSVELAEEQKKLEEEKKEAEKESKDEEKIDE
                     DKKEEKKDDEKKEEGYKPGEIRIRVSIPRDIPREQILLTGARLITMKGDEIIERGDIW
                     IENNRIKAIGASGSIEVPSNVTRMDMTGKTIIPGFVDTHSHMWPEWGIHKNQIWIYAA
                     NLAYGVTTTRDPQTATTDVLTYSDHVTAGNMMGPRIFSTGPGVGFWANNIQDLDHAKK
                     VLRIYSDYYDTKTIKMYLVGNRQQRQWVIMAAKELGLKPTTEGALDLKLDFTQIIDGY
                     PGHEHSFPIYPLYNDVVQFVTQSRTTYTPTLLVSYGGPWAENYYYATETVHDDPKLSY
                     FTPHSELDSKSRRRGSWFMKEDHVFEDHAKFVKDLVEAGGLAGVGSHGQLQGLGYHWE
                     IWSMQSGGTSNHNMLKVATILGATGIGLNGDIGSLEMGKLADLIVLDKNPLEDIRNTN
                     SILFVMKNGRLYEGNTLNEVYPLKKDAGKFWWKQPEPGNIPGIR"
     gene            6052..7488
                     /locus_tag="VGA21_04180"
     CDS             6052..7488
                     /locus_tag="VGA21_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013409192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HEX9649732.1"
                     /translation="MKIIVCIPFLLWISCTPRKISAPAPVFPIPSPEQFTWHNLEFYA
                     FIHCSINTFTDKEWGFGDENPKLFNPDSLDVKQWVDVFKEVGMKAVIITAKHHDGFCL
                     WPSAFTEHSIKNSLYKNGRGDILKELSDACKKVGLKFGIYLSPWDRNHSRYGHPEYID
                     YYRNQLKEIFTNYGPVFEMWFDGANGGDGFYGGSNEVRRIDRATYYNWPGTIKMVQEM
                     EPKILFFSDAGPDIRWVGNERGIAGQTNWNTISADTLYAGKPGITELLNTGSEEGINW
                     IPAEVDVSIRPGWFYHESEDTKVKTPEELFAIYLTSVGRGSNLLLNVPPDKHGRIAKE
                     DIRSLRGLKELLDEKFSRKIVASHITSSNYRGNSKNYRPENLIDSDYDTFWSTDEVEI
                     NATIEIGFEDLTEINFIVIKETIQLGQRIKKFTIEAKVGADWKKVAEGTTIGYKRILP
                     LAGIQTNKLRIKVVDAKACPVISEIEIY"
     gene            7497..7883
                     /locus_tag="VGA21_04185"
     CDS             7497..7883
                     /locus_tag="VGA21_04185"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold thioesterase"
                     /protein_id="HEX9649733.1"
                     /translation="MDDLRKKIQTDPYLNFLGIELEELSDGYAICSLSLSKDMVNFMG
                     VPHGGLLFSLADAALSAASNSDHYPSYAIEVNGSFLAKVSIGDTLTAKSVRIHQTHKT
                     AVYKMEIFHLEKIIAVFRGTVYRSKK"
     gene            7880..8524
                     /locus_tag="VGA21_04190"
     CDS             7880..8524
                     /locus_tag="VGA21_04190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9649734.1"
                     /translation="MKLFLLSGLGADSRVFDFLDLPGIELKPVEWLMPLPKERISDYA
                     GRLSEALKPYEPVNLLGVSFGGIIAQEMATHLDTRKVFIISSIKSSAEVGLAFHITKR
                     LGILRLISPKKMKQSMMKMGPWLFGLEKGSEVKLFRNITTDTNEEFLKWAVCQLHGWK
                     TSSSNLDVIHIHGIKDKMFPINKIKNFIPIEDGGHFMIVNRAEEISKIILEKIK"
     gene            complement(8549..8974)
                     /locus_tag="VGA21_04195"
     CDS             complement(8549..8974)
                     /locus_tag="VGA21_04195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649735.1"
                     /translation="MDTSKFIKGTIVGGVAYFFLGFLIYAVLLEDFFINNAGTAIGVT
                     KTEMEWWPLVLGNLSLAALFSYIFLKWANITSFGDGLKAAGILGFMIGLSFDMVMYDT
                     SNIMNLTAAFVDVIAFTVMTAIGGGVIGAVLGMGAKSAS"
     gene            9377..17776
                     /locus_tag="VGA21_04200"
     CDS             9377..17776
                     /locus_tag="VGA21_04200"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9649736.1"
                     /translation="MKRREPLAIIGIGCKFPGDINSPDDFWKLLIEKRDALTRIPADR
                     WDADGLYAPEFRRAGKISVNRGGFVRDVDKFDAGFFGISPMEASRMDPQQRMLLEVSY
                     EAVQDAGIRLNDLEGKKIAVVIGISAHDYGDIQNSPSERVNIGAHTNLGSALCITANR
                     ISYCYNLKGPSFAIDTACSSSLNAIHMACRSIWDGDAEGAFAGGVNSILKPEPQMGFS
                     KGGFLSPNGTCFSFDDRGDGYIRSEGAGLVFIKPLVQAEADNDPIYAVIRGTAINQDG
                     ATKGISVPNPIAQQELLVEAYKDAGVSPHEVTFVEAHGTGTFVGDPIETNSIGKVIGG
                     KRKTVCYIGSVKSNIGHLEPASGIAGLAKMALALKNETIPPNIHFKKGNPNIPFDDMM
                     LKVPTEPIKWKDNGKPRYGGVNSFGFGGSNAHVILESIRQNIQKNDKPGLQIATISAR
                     SQDALTALTKDFIAYLKDKKNKASLSDICYSTAVRSTHHDIRLAVVAESKKELARNLK
                     IFLAGESLTEIAEGQALEIKDKIAFVFSGQGPQWWGMGRQLFENEPLFRETIIKLDKM
                     LGKHASWSLIEELTKDEKTSRISETNIAQPAIFSIQIALYEMWKALGITPSAVVGHSI
                     GEVAAGYASGALTLEQAVKLIFHRSRVQHKATDKGRMLAVGLPLEEAVKLINGNEGRV
                     SVGAVNGPSMVVLSGDTDVIEEIAEVLDKKDVFQRLLAVNVPFHSHHMEPLKNELITS
                     LGSFKTTSTQVPFYSTVEGKKVKGEDLNPTYWFRNVREPVYFTSAIESLINDGFDTFI
                     ELGPHPIHAIGINDLISQKKSKALVVPSLRRNENEKRTFLSSVAALHTWGCQIDWKLF
                     YGNDNGFVKLPRYPWQKESFWLETEEGKRLRLGTIEHHPHLERKTSSPRESHNILWNV
                     NLDKRSYPYIEDHKVQGPIVFPGAGHVDLVLGAAMASFGDKFISLEDINFENALFLPD
                     SGEPPEIQIDISHDAGDYYIYTKPRGIEAPWTMCSNGKMNHTGEKFKSIPINLNEVRK
                     RTIIPVPVKAMHDELLESGLYLGPSFRAIKKLWRSDGHWESLSEIEVHESIRSEFLQF
                     NIHPGVLDSCFQTVFGIFNEREDVNKKMGVYVPRHIDKIKFYTKPNSFKLVVHGRLRE
                     WTDEYALGELWIFNTDGSLVAEFHGFLSQYLKGSRGETGSEQEKWFYEYNWVMKPRAD
                     QELLRNPGNYFQSPTKIKSKVKETIKKVVTRPEQREYYKHYEPEQYALTIEYICTSLR
                     ELGFSFKTGQKADLKYLIDTYKIIPQHFRLFHHMFNLLKDAGIVKGKDGHYEIVKNPK
                     ERDVNSWMDDLQKKYPQFHHESTLLGRCGPHITGVLQGTVDPIELIFPEDKWDAIVKY
                     YVEGFAFRKYNEMAARVVKELLARLPKDQTLRILEVGAGTGGMTQAILPLLPADRTEY
                     IFTDLSHMFMLKAQQRFSNYPFVQYKIMDIENDPVEQGFTQNSYDLIIASDVIHATRN
                     LGISLGNVKKLLASEGVLMMLEVTNSPVYLDFIFGMTEGWWLFEDTDIRPTHATMDPA
                     RWKMVLEKNKFNDVAIYSDFERNDSSCQSVIVARSPRIDLSKLVKENPGKKARANWLV
                     FADDKGVSDKIMNHLKKLNKNCIVIHRGGDFSESTKNEFTIDSLDQEGADKLIEYISD
                     EDNFQGLIFAWGLDSADNNKLSESTIIKAEEDSSAMMMNIMRKLNKMNYEKNPDIWVL
                     LRGTQVVGDSPTTINLSQQGLRGVSRVIVNEFPNYKTSLVDFSSQVKEEEIQSFIDEL
                     FNDEHVDEIAFRGKKRYVNRLERLSTRKIANLSRKTVNAEGTPYVAGISEYGVLDNIS
                     LRQTSLRKPEEDEVEIRVKASALNFRDIMIAMGLLSDAAVEGGLFGRTFGLECAGVVS
                     AVGKKVKSVKVGDAVMATAPSCIAGFAYPKGSHVVKKPKNISWTEAASLPVVYTTAYF
                     SLVYHCRLQKGEKILIHAAAGGVGIAAIHIAKAIGAEIYATVSSPDKKAYIRKIGVKS
                     ENIMNSRTLDFADEIMEKTGGQGVDVVLNSLSGEAIYKSVKCLSPYGRFVEIGKTDIY
                     RNSKLGLQPFGNNLTYFGVDVDRLFKQKEKFGGKLFQDSIDYFVKNKFPVHPVKVFSI
                     DNLADAFQHMAGARHIGKIIIEMKGEVQISPPREIAFDANGSYLLTGGASGFALSVAN
                     WMTTKGARHLVLMSRSGPKTNSEKEIVARMKKRGVKVMIAKGDVSNKKDVDRIFAEIK
                     KEMPPLKGIQHAAMVLDDGGIPEIDHLRYLKVFKPKAIGCWHLHNASKALKLDHFISY
                     SSISAIYGNPGQVSYVGGNSFLDNFSSYRRSLGLPATTVNWGVIGDVGFVARSGKVGG
                     LLYKQGWKAFTLDQALGILEQMLLNNPIQRVATDSDWEMIGNFYPHSATSSRFAHLVK
                     EKELGGAGGVGQGDCALKATLLESSSEEKPEILLTALKDTFARVIGTTADKVETSQPV
                     TKYGLDSLMANQIRNWIQSNIAVDYSMMRIMRGPTMEEMTAQILDELTGQTGSSNGLL
                     EERTELDKWIIRTKRVENPRLRLFCLPYFAGGASIFSSWHEFLPEDIEVCAIQFPGRE
                     ERGDEKAYDDVFALVKKLAEVIEPLLTAPIAFYSHSSGAGIALELARHLRKTNDVNPV
                     KFIVGGWRAPHLSSPFKFLNAIKEDEVYKEKNIPNIKNHLRSLEIPDSVIDNKEVFGE
                     MLPSLRADILLGKKYKYYEDEPLPCPLVGVAGTKDTVFTEDQIKAWSKHTSSEFTFKK
                     INGSHLFCRDNKEELLEFLSHELVDNVIA"
     gene            17781..18524
                     /locus_tag="VGA21_04205"
     CDS             17781..18524
                     /locus_tag="VGA21_04205"
                     /inference="COORDINATES: protein motif:HMM:NF013786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HEX9649737.1"
                     /translation="MKSVKEVITSPFELLRKFNSAIGQGEVHLYACKLDPGSERINYL
                     ETLLSEQEKKNVSFFSFEKDQIAYTASQGFLRLVVAFYLDIDGSEVKFAKHRKGKPYL
                     LNDTSLLYNISNSGDYVIIGISRHGEIGIDLEKIRPLDDFEDLVHKNFTPREREYIGK
                     VPTETINRFFKLWTLKEAFLKAIGEGMRLTPDNLEFLIENGKFKLHTGPGFYEFDDWH
                     FSDITIGNNYLGSLAHMKNPVYVSEIRYL"
     gene            18521..20323
                     /locus_tag="VGA21_04210"
     CDS             18521..20323
                     /locus_tag="VGA21_04210"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEX9649738.1"
                     /translation="MNYAVTRTFDLLNQYQEKFPDKPDALAGIENKKWVTFSSKEYIE
                     NSNLLSYGLLALGIKKGDVVATITNNRPEWNFVDMGIAQVGAIHLPIFTTLDSEGYKH
                     ILTQSRPKVIFLSNASLAVKIEFLKNDLPETSLFTFDKEENFDHWLKIIESGKQNEAI
                     LKDQVESIKKEILPKDWVTLLYTSGTTDNSKGVMLSHENLMTNAAAAAEVFNLKPEHK
                     YLSFLPLCHVGERMANYQTQLTGCSLYYAENIGTIARDLKDLKPNGFGAVPRILEKVY
                     DRIMEKAEKLTGLKRWLFFWAMRLGYKYDPSVTKGFGYNCQLWLANKLVFSKWREALG
                     GNVQFIGVGGAMLQEKIERVFWAAGIKLLNMYGLTETSPIITINRAKGPLLKVGTVGA
                     PIKDVEVKIVDDGEIVCRGPNVMLGYYNNETATKEVIDSDGWFHTGDIGIFEDGKFLR
                     ITDRKKEIFKLSNGKYVQPQTIENKLKESPFIDQCMALGVGEKFASALISPNKEALKS
                     WAKTNNINESNVQTLVRLKPVIEFYKTIVDEANNSFDKDEKLSRTKLIGDEWTPDTGE
                     LSPTLKLKRRVITEKYRNLINEIFLKDERFRFKL"
     gene            20320..21486
                     /locus_tag="VGA21_04215"
     CDS             20320..21486
                     /locus_tag="VGA21_04215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649739.1"
                     /translation="MKIVEVQNNKHENQFLQVARELYKMDPNWICPLDNDIRSVFNPL
                     ENSYFNHGQATRFILENNNNVIGRIAAFIDKKTADNSELPAGGCGFFECVNDQAAADL
                     LFNSAKEWLLQRGMQAMDGPINFGETNKYWGLLVDGFSQPAYQIAYNHPYYQKLFEDF
                     GFKIYYKQEGFHFYLDREIPPRFWKIASWVAKKPDYSFEYLQFSKVEKYVKDFTKVFN
                     QAWADFKEDFEPLEEAYVRNFIMKAKVIAEEKFIWFAYNKGEPIAVYLMIPDVNQIFK
                     GFKGKLNLLNKLKVLYGIKTKKITRARGILMGVIPSFQGLGIESAFIYHLNEVFKKMP
                     HYKEIEFSWVGDFNPAMRKLWLSVGAEPTKQYITYRYLFNREAEFNRYPIPVEK"
     gene            21565..21915
                     /locus_tag="VGA21_04220"
     CDS             21565..21915
                     /locus_tag="VGA21_04220"
                     /inference="COORDINATES: protein motif:HMM:NF016854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HEX9649740.1"
                     /translation="MPFDQNLADRIHTILNSRKIKAVEKNMMGGLTFMVNDKMCVGIV
                     KEDLMARVGPYHYGKALKMKGAREMDFTGKPMKGFVFVAPDGIDLDEDLEKYLPMALD
                     YNKEAKSSKRKKIR"
     gene            22188..23366
                     /locus_tag="VGA21_04225"
     CDS             22188..23366
                     /locus_tag="VGA21_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009186764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEX9649741.1"
                     /translation="MADKAFITPKVLKWARESARMTEETAAAKVSVTIEKVKEWEAGA
                     SQPTIRQAQTLAKAYKRPFALFFLPEIPRDFQPLQDFRKSGSKAFTTSSVFIIREIQQ
                     KQTWISDVYSENQEEKLPFVGRFSLNDNPQKVAQDILSTLKINPATYKSDNPIKEWID
                     AAETNGIFVSRTSFIHSRLKLDSEELQGFAIADPHAPFIFVNSDDWNAPQLFTLVHEL
                     AHIWIAETGISNEIEPDLKHKDKFHTVELFCNGVAANALMPKEIVLSFDSSSFQTSKD
                     VFKVANQLGVSSFALLVRVLNLNIISTPAYQKLKRQADIDYADYLRRKAEKKARQKEK
                     EKPGGPNYFLLQLNRNSRLFTQTVLDAFRGGFIEPTLASNLLNVQVNKFQKLESQLFR
                     "
     gene            23363..23869
                     /locus_tag="VGA21_04230"
     CDS             23363..23869
                     /locus_tag="VGA21_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020599146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4411 family protein"
                     /protein_id="HEX9649742.1"
                     /translation="MSINANKYCLDANVLIQAWQKYYNPKFCLDYWNILIELGKQDRI
                     FTPELVSEEITRTEDDLSKWLKGSKIPIKKISEPVTICLQRIYSTNPIHQNLVDNTKA
                     RSLADPWLIAHALYENATVVTKEEKITALNSSRIKIPNVCDNMGVRWINDFQLIDELE
                     IKFKCSLT"
     gene            complement(24035..25489)
                     /locus_tag="VGA21_04235"
     CDS             complement(24035..25489)
                     /locus_tag="VGA21_04235"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HEX9649743.1"
                     /translation="MILPELKLNLNFHIILILIATSSCMDPATKSEITRPNIVVILVD
                     DMRWDEFGLAGHNYIKTPNIDRIAREGIYFRNAFTTTPLCSPSRASFLTGQYAHTNGI
                     TDNLARNEQSHRLKTFPLTLDSAGYETAFIGKWHMGNDDSQRPGFDTWVALEGQGEAI
                     NPNLNINGDKKNIEGYVTDVLTEFSTEFISAQRSQPFLLYLSHKALHPNMKQKDDGSV
                     EMIGSGGFIPAERHTGMYGDKVFQRRPNAYLPPKDKPSLMRQIDALPQLGRETATDEK
                     TIRERSEMLMAVDESLGRIIDQLEESNLLENTIIVFTSDHGYWYGEHGLSVERRLAYE
                     EAIRIPMLIRYPRVFEPGQTNERMILSIDLAPTLLDLAGLIPGAHIHGRSMVPLMNGK
                     TQDWRSSFLIEYYSDTVFPRILKMGYKAVRNERYKYIRYLDLKNMDELYDLTEDPYEL
                     NNIHDAPGSEEVLKVMKAELNRLLLKTDGAQIGS"
     gene            25754..25975
                     /locus_tag="VGA21_04240"
     CDS             25754..25975
                     /locus_tag="VGA21_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013454458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HEX9649744.1"
                     /translation="MKKNMGTADRIIRLIIAAILGGLFYAGAISGAFGIVLLVLAVIF
                     ALTSMVSFCPIYKILGTNTCPTETSKEKL"
     gene            26026..26550
                     /locus_tag="VGA21_04245"
     CDS             26026..26550
                     /locus_tag="VGA21_04245"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX9649745.1"
                     /translation="MLNFDFNPFPILETTRFSLRPISEIDIDEIFELRSDPGVNKYLS
                     RKTARDKKEALEFIKSIIAGIFAGEWIYWGISFKDEQSLIGTICLWNISKEKKEAEIG
                     FELLQSFQGNGFMKEIAPAVTRFGFEKIRLLTINAITHPKNKRSIRILESLGFTYLKQ
                     ESAKESLFIKKAFN"
     gene            26641..27291
                     /locus_tag="VGA21_04250"
     CDS             26641..27291
                     /locus_tag="VGA21_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002700422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEX9649746.1"
                     /translation="MNSEIMELLMGNREWVERTHQKFPDFFENLAKGQSPKFLWIGCS
                     DSRVPATEVTNALPGSIFVQRNIGNIVVHTDSNLLSVVHYAIKVLKVKHIIVCGHYGC
                     GGVLSAMGNNHFGFLDNWLVHIKDVYRLHKPELDAIEDTTKRGDRFVELNVKEQVNNL
                     SMVSFIQEEWESGEYPYIHGWVYSLNDGLIRNLGISINSSEHLDEVYQYSAMKRTE"
     gene            27320..27865
                     /locus_tag="VGA21_04255"
     CDS             27320..27865
                     /locus_tag="VGA21_04255"
                     /inference="COORDINATES: protein motif:HMM:NF019614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HEX9649747.1"
                     /translation="MGVYISILRGINVSGQKLIKMVDLVKHFQSLGMKQVRTYIQSGN
                     IIFQCSDSAPGKIALKIEKKIKKEYGFDVPVIVKSAKDIEKTLNNNPFLKRGESSNQL
                     YVTFLSDVPKRELVNSINAKQYLPEEFIWDKDRIYFFSPHGYGNAKMNNNFFENKLKL
                     KATTRNWKTVIKLFELAAQAD"
     gene            27965..28483
                     /locus_tag="VGA21_04260"
     CDS             27965..28483
                     /locus_tag="VGA21_04260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649748.1"
                     /translation="MKRIYFYFFGILLISACEDDDKYFQNFENTRDASVPAVTSYDGS
                     LKVSLNDENNIPITKLDLRIHLEYSKALSTTTTHYYKVQGTGLYSCNSSVNGDAIISE
                     GIFSVRKSDPERFIALTFSGGKLANQTLRLNLSEIDGNEISGKWFNVLDSEGGTLFGN
                     TDCQRGLLSLPK"
     gene            complement(28535..29848)
                     /locus_tag="VGA21_04265"
     CDS             complement(28535..29848)
                     /locus_tag="VGA21_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015330855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX9649749.1"
                     /translation="MKTRNIITILLMAVCTMSYAQYPLIPAAAQSRPVLLKGATAHLG
                     NGQVIQNSAIGFENGKLTIVADATAVRIDETGYEVIDVSGKHVYPGFIIPNSSLGLGE
                     VGNVRSTNDLNEEGDYNPNVRSIVAYNADSEMIPTYRFNGILMAQITPQGGTIPGGSS
                     IVQLDAWNWEDAAYKLDEGIHLNWPGKRRGNFDFTTFTFSFTDNENYPRQVQELEVLF
                     RDATSYTKLVAPKAPNLKLEAMKGLFDGTKTLYIHSNNAKEIVESIRFAKEQGVQKIV
                     LITADQALFVTSFIKENNIPVIIEEVHNTPSTSGTDYDLPYRLPSMLQKEGIKVSLGY
                     SQGSAPQSARNLMFTAGTVAAFGGEKEEALKMITLNTAEILGIDDKVGSLAVGKDATL
                     FVSEGDALDMKTSILNQAYIQGRRITLDGMQQSLYKKYKEKYGIK"
     gene            complement(29867..32902)
                     /locus_tag="VGA21_04270"
     CDS             complement(29867..32902)
                     /locus_tag="VGA21_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012926366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX9649750.1"
                     /translation="MRKNLLLLFGLLFWQGNLLAQETFPVNDVQDVRTKAYALTNATI
                     YVDYQTKIDKGTILIRDGKIENVGANLSIPAGYIQVDLAGKFIYPSMIDPFTSYGLPE
                     VERRRGGGFGGREQINSDTKGAYNANEAIKSEYNASDEFSPDSKSGKSLRDIGFGSAL
                     TFRADGLARGTGAFVTLGEETANKELLVSVAAANYAFDKGTSSQQYPSSRMGFTAVLR
                     QTRFDAEWYNSLPNKTFTDKSLDAWLTTQRYPQLFEVSNWKEILRADKLGDEFGTQYI
                     IKGNGDEYRRINEIKATNASIILPINFPEAVDVDDPVDALRVSLEDLKHWELAPANPA
                     ALEKAGINFALTTDDLRAKNNFWTNLRKAVKYGLSEQGALKAMTFTPANLLRVYDKVG
                     SLAAGKYANFIITNGNIFSDDAIIFENWIQGKPFVLKTDDKDYSANYTLSVNNSSQSL
                     EVSGEAGKNTFKIKVNDSTNISVDAKIDGDLITLLYKPDKDAKGMVRLSGWFSGTPGQ
                     QGSGWNGKGQLVNGNWIDWSATFASAASGGNNRKDNSSDDTPSDLGKVVYPFMAYGNE
                     TVPVVETLLIKNATVWTNESDGILENTDVLLQNGKIARIGKDLTVAGAKIVDGTGKHL
                     TSGIIDEHSHLANDGINDVVSNSGMVRMKDVIDSEDIGIYRALAGGVTAAQILHGSAN
                     PIGGQSGLVKFRWGKSPQEMLIENADEFIKFALGENVKRSTNAQSIRFPQTRMGVEQV
                     YMDAFTAARDYDKQMKAYNALKNKSGVVKPRRDLAMETMAEIINKQRFISCHSYVQSE
                     INMMMKVAGKFNFNVNTFTHILEGYKVADKMAAHGAGGSTFADWWAYKWEVRYAIPYN
                     PALMAGAGVTVAINSDDGEMIRRLNQEAAKSVKYAGMSEEEAWKMVTLNPAKLLHLDN
                     RMGSVKVGKDADVVLWNENPLSIYAKPLKTIVDGVVYFDVEKDMEMRKWIEAERARLI
                     AKMKDAKAGGARTARPFSRPQRDFHCEDFTGYEYLFENK"
     gene            33053..33880
                     /locus_tag="VGA21_04275"
     CDS             33053..33880
                     /locus_tag="VGA21_04275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649751.1"
                     /translation="MKKILNFRLIIILMLIGACHSRDKSLKEDLALKDTLTGGWITSN
                     SNANPLGFVFLPNGRFEAENFARGPSGVWILRSDSLIILMRTVEQDQKFSFKMRFENG
                     ELLLNNSSDQMRFSRRPYGVNPVFLSDRFESITRGINQRNLNQAENIMELPASGNSEG
                     SQILGLWENENTPEKLAATEPVKDNVFAGFSEYYFIEGHLICKKGPMENLLFENNEIV
                     LWTDSLLNILQPTEIDLKSKAEVELEKVSRYLKAYGIHEDNGVFFRIEESGVSAPDS"
     gene            complement(33944..34141)
                     /locus_tag="VGA21_04280"
     CDS             complement(33944..34141)
                     /locus_tag="VGA21_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013454244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDCCVxC domain-containing (seleno)protein"
                     /protein_id="HEX9649752.1"
                     /translation="MKKLITFESVITCPVCGHQKKEIMPEDACQFFYECENCNTVLRP
                     AAGDCCVFCSYGTVKCPSKQS"
     gene            complement(34345..34686)
                     /gene="arsC"
                     /locus_tag="VGA21_04285"
     CDS             complement(34345..34686)
                     /gene="arsC"
                     /locus_tag="VGA21_04285"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007660417.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HEX9649753.1"
                     /translation="MVTIYHNPRCMKSRQTLALLEKREKNIEIIQYLKDTPDSKELEG
                     ILDKLDLDAEDIVRKGETLYKEKFKGKQLSRKQWIEALVKNPVLIERPIVVKGRKAVL
                     GRPPENVSKLY"
BASE COUNT        10627 a         6834 c         6996 g        10280 t
ORIGIN      
        1 tagattggat aatcaggaat tcgggcaatt gtgaagatga tctggaaaaa ccatcggtta
       61 tgattaagga atctaaaacc agaaaaataa gagaagcggt taatgaattg ttaagcggac
      121 aattggttag ttaatgcatt cggactgcca cgccaggaat gcaaagtctt catttcgttc
      181 ccgcgcctca tccaattttt tacaagctga tttcaaatct ccggaccggt agaacgcgtc
      241 tgcccaaaag gaaaaaaggg attccacact ccctccccta atggaagctt cataaaaaag
      301 ttgtaatgct ctattataat ccttttgttc cagatagacc aatgctttat ttcgataagc
      361 ccaaccatta tccggattaa ttacaatgct ttgatttatc agcttaagcg cactatttat
      421 gtctcctttc ctgaatacaa taagtccata gttattgagg taatatgaat ctttaggttg
      481 cagcattaag gctcttttta tatttatttc tgcactatcc agattccctt ttcgtaagta
      541 aaccaaagcg atggaattta atgcaggtga aaaagttgtg tccagattca gagctttggt
      601 taaatcggaa attgcccggt tgagttgatt cttataatag taaactgttc ccctgtttgt
      661 ccataactcc ggattgtctg accggtaggt tattgccaag ctaaagtaat ttatagcgga
      721 gtcgtacttc tttaactggg tataaatcaa accctttctg aagttaatat gaaaagtgtc
      781 gggagttatt tcttcaacaa tttcaatatc ccgtaatgac gattcaaata gccctatttc
      841 gtagagagcc gccgatcggt tcagataggc gtcaggaaaa tcaggtttaa gagcaatcgc
      901 cttgtcataa agttccaggg ccaggtaata tctctgctgt ttgaacctga taatcccaag
      961 attattcaac gcatggggga attctggaaa ttcagtaatg gcctggttat aataatcttc
     1021 cgctttggcc aattcacccc tgctaaggtg ctcattgcca acttttaaca agtcctgagc
     1081 acgttcagcc gggtttgagc aacacaataa cccgaggctc aatagtgata atatcggtat
     1141 ataacttttc aaaatatggg tgaaatggta caatattacc tgataatggg atcaaattac
     1201 aactaatcca gactccttgc ctataccttt acactcgcag taaaactcag gtaattctac
     1261 tctataaggc cggataatca aggattttcc cggccttatt ctttttacag acatgaagat
     1321 tttatcagat tttatgtaga ggaaaggtaa aaacaattat cttttgaagt taatcgaaaa
     1381 gctttaattt agactcaact gtaaattatc caataatcct atcaaccgtc gcctatgctt
     1441 cttttaggga gtattgtagc cttagtggtg tctttttatt tactcgccca ggtaagtgat
     1501 aaatacttca ttgaatccct cgataagata gctcacagat tcaacatgtc acatgacatg
     1561 gcaggggcaa ctttgatggc aataggatcg agtgctcctg aactttttgt ggccatcatt
     1621 gctttggtaa agccaggaga tcacggagaa atagggattg gcaccatagt aggttcggcg
     1681 gtttttaaca tcctgggaat tattggtgca gttgccgttg tacgaaaatc cgttttggcc
     1741 tggcagcccg tcttaagaga cttgatcttt tattcgatct caataattac cctcattgtt
     1801 gttttctatg attctcaaat tgaattatac gaaggagcca ttctgatttt catttatgga
     1861 ttttacctgc ttgctgtttc caagtggcgc aaattgtttc cctatgagga attggacacc
     1921 attgaggagg aagaagaggg agagggagaa gaagaggcac cagtttggga gaaagtattc
     1981 cggccgtttg attatgcaat tgataaactt tttcccccga tcagatatta ttatggggtt
     2041 ttctttattt caataatagt aattgcactg ctaagctggg tgctcgttga aagtgctgta
     2101 ataatttcag aaatgctaaa tattccaaaa gtggtaattg cattaacggt tttggcaatt
     2161 ggcacttcag tacccgactt aatgtcctct gtaatagttg caaagcaagg cagaggtgga
     2221 atggcgctaa gcaatgccgt tggatccaat atttttgaca ttcttatatg tttggggctt
     2281 ccctggttac ttattattag tttttccaaa actccattac ctgtaaagac ggatgatttg
     2341 tttgtcagcg ttattctgct tttttcttcc gtattgataa ttctggcatt attagttatc
     2401 aacaactggt ctattggcaa gaaagccgga atattcttat tgctacttta cctagcctac
     2461 gttatacgag aattttacct ggcgatataa aaactcccgg aaatctctgg aggctttttc
     2521 atcaacgaaa gattatttac cttattcccg gtatgtttcc cggttcaggt tgtttccacc
     2581 aaaactttcc ggcatccttc tttagagggt atacttcgtt taaggtattt ccttcataaa
     2641 gccggccatt cttcatcaca aataatatgg agttggtgtt tcgaatgtct tccagggggt
     2701 tcttatcaag tacaatcaaa tctgccagtt tacccatttc caaagatcct atatctccgt
     2761 tcaaccctat tcctgtagca cccaaaattg ttgctacctt caacatattg tggttggatg
     2821 ttccacccga ttgcatactc caaatttccc agtggtaacc aagtccctgt aattgtccat
     2881 ggcttccaac acctgcaaga cccccagcct ctaccaaatc tttaacaaat ttggcatgat
     2941 cttcaaatac atggtcctcc ttcataaacc agcttccccg tcgtcttgat ttactatcta
     3001 attcagaatg aggagtgaaa tatgaaagct ttggatcgtc atgaaccgtc tccgttgcgt
     3061 aataataatt ctctgcccat ggccctccat atgaaaccaa taatgtggga gtgtatgtgg
     3121 ttctcgattg ggtaacaaac tgaactacat cattataaag gggataaatt ggaaatgaat
     3181 gttcgtgtcc cgggtaacca tcgataattt gggtgaaatc cagcttcaaa tccaaagctc
     3241 cctcagtggt tggttttaat ccaagctctt tcgctgccat aattacccat tgcctttgct
     3301 ggcggttgcc taccaggtac attttgatcg ttttagtatc atagtaatca ctgtaaattc
     3361 tgagaacttt ttttgcgtgg tcaaggtcct ggatattatt tgcccaaaag ccaacgccag
     3421 gcccggtaga aaatattctt ggtcccatca tattgcctgc cgtaacgtgg tcagaataag
     3481 ttaaaacatc ggttgtcgcg gtctgaggat ctctggttgt tgtgacaccg taagccaggt
     3541 tggcggcata tatccaaatt tgatttttgt gtattcccca ttcgggccac atatgcgagt
     3601 gagtgtctac aaaacctgga attatcgtct ttccggtcat gtccatccgg gtcacattgg
     3661 aaggcacttc aatactacca gacgccccaa tcgcctttat ccgattattt tcaatccaaa
     3721 tatcaccacg ctcaataatc tcatcgcctt tcatcgttat gagtctggcc ccagtaagca
     3781 aaatttgttc ccttggaata tccctcggaa tagaaactct tattctgatt tcacctggct
     3841 tatatccttc ctcctttttc tcatcatctt tcttttcctc ctttttatct tcatcaattt
     3901 tctcctcatc tttactttct ttctcagctt cctttttctc ttcttctaat tttttctgct
     3961 cttcagccaa ttcaacacta tcctcataaa cctttgcagc agttatatca tatttaaaaa
     4021 acgcgttgcc cagagaaaaa taaactttct ttccatcact tccccaggtt ggaaactccc
     4081 ccccaatatc agtaactttc ctggagggaa attgcgcctt gtccggatct gatactgaaa
     4141 ctttgggagt ttcccctcca acatagggta ctgtaacaac atagacatca ttattcacct
     4201 gtgccattgc ctggtcccct tccggggcca tcataattaa atcagccgtg cttgggttct
     4261 gggctccgaa agtcgtaata cccgtaactt ttaaatgtgc cttctcatca gttccatccc
     4321 atcttatgga aaccaatccc ttaccattag ttcccccctg atataaataa atcctatcct
     4381 ggcccttcac aaaatgtggt gttaatcttc ctcgtgtttt tgtgataaat ctctcaggac
     4441 ccccattcgc tgaaacccaa cccaaatcaa cacttgtgcc tgaattaaat ggatctacag
     4501 attccttata aatctgtgct gcgccgcgta catatacgat cctgttattt tctgcccagg
     4561 ctggagttga gtatagcgct gcagtctgag tcaatctaac cggagatggc ttccctgaaa
     4621 cagttacttt atagatatgt ccaccttcat tagaccatgt gacataagca atccattttg
     4681 aatccggaga ccaggcaggc attgcttcaa catcgtttga gtttgtgagt ttgacaggct
     4741 ttccatcagg atagtccatc acatagattt tatcaagtac agtaaatgcc gctttttttc
     4801 catcaggcga aacagcagga tcacgaattt gccgggcgat aaattcagta ctatcggaaa
     4861 tcgggtattt aaagtccagc tcaggaccca attccaagtc aacatcaata tccataggaa
     4921 taataactgc actccctcca ttaaccggaa tcttaaacaa tttgcctcca taggaacaaa
     4981 ttagaaactg attatcattt gtgaacgaca ttcctggtaa aacatccatg gtggcgatag
     5041 attcctgatc atccctctga acaggatagg ctaaccactt ctcatcacca gttgccagat
     5101 ttctgataat caatccggtt ttatcatcat gtctggttcc atatgcgagc catttaccat
     5161 ctggacttgg tgttggcctg aatcctgaac catatcttga tgttatggtt tcagactctc
     5221 ccgtttcccg atcataaata gcaatctggt actggggaaa ttgtgcatta tagttccatg
     5281 atccaaatct tctggaatag tatataaatc ttccatccgg actgaaggca ggttccactg
     5341 tttttagatt agttggtcct tcaatcaagc cagtccctga tccaccatca acatgaaaaa
     5401 gccacaattt tggaactccc aaaagtgctc ctcctttgga tgcaactata tatttgctat
     5461 caggagaaaa gtcggcactc tggtaagaat tgtttttccc ttcactgatg gcttcaaatt
     5521 taatggtatc aggttcaagt tttgctatcc aaacattttc cgccccactc ctgtcggaaa
     5581 tgaagactat ttttttaccg tcagggctat atctgggatg cgaatcaaaa gccaggccgg
     5641 atgaaattct ggttgcctta ccaccagctg ccggcatagt atacaaatca cccaataaat
     5701 caaaaactat tgtgctgcca tccggactaa tatcaacaga aatccaggaa ccttcccggg
     5761 tattaaattt gatatgtcgc ccaacttcaa gcgggagatc ttttttctct ttcttttctt
     5821 ttttggtgct gtccgatttt acggtatcct gctcaagaaa aatatttgac tcagcctgct
     5881 ggtacaaaaa taccgctata agacaaaggg taaaccgtgt tacaaatttt accatgatgt
     5941 ttaatttaaa ggttttatct tcggaaatgt tttcaataat aatgaatgac aaataaagga
     6001 aaaaaatccg ggcattataa cctcaagcgc ctattaaagt tgagaaatgc tatgaagata
     6061 attgtctgta ttccattttt gctttggatt tcctgcactc ctcgaaagat ttcagctcca
     6121 gctcctgtct ttcccattcc tagtcctgaa caatttacct ggcacaacct cgaattctat
     6181 gctttcattc attgttcgat aaacacattt actgacaaag agtggggatt tggtgatgag
     6241 aatccaaagt tatttaatcc tgactccctt gatgtaaagc aatgggttga tgttttcaaa
     6301 gaagtgggaa tgaaggcggt tattattaca gcaaagcacc atgacgggtt ttgtctctgg
     6361 ccaagtgctt ttacagagca ttccattaaa aatagccttt ataaaaatgg ccggggagat
     6421 attcttaaag aattatccga tgcttgcaaa aaggtgggtt taaagtttgg aatttaccta
     6481 agcccctggg atagaaacca ctctcgatat ggacacccgg agtacattga ttattaccgg
     6541 aatcaattga aagaaatatt tactaactac ggtccagttt tcgagatgtg gtttgatggc
     6601 gcaaatgggg gtgacggatt ttacggtgga agcaatgaag ttagaagaat tgaccgggcc
     6661 acttactata attggcctgg tacaatcaaa atggttcagg aaatggaacc aaagatactt
     6721 ttcttcagtg atgccggtcc ggatattcgt tgggtaggca atgaacgtgg cattgcaggt
     6781 caaacaaact ggaatacaat ttccgcggat actctttatg caggcaaacc tggaattaca
     6841 gaattactta acacagggtc tgaagaaggt atcaactgga ttcctgcgga agtggatgta
     6901 tccatcaggc ccgggtggtt ttatcacgaa tcggaagaca ctaaggtgaa aactcctgaa
     6961 gaactatttg ctatttattt gacatccgta ggccggggtt caaatcttct tcttaatgtg
     7021 ccacctgaca aacacggtag gatagccaaa gaagacattc ggtcgctgag gggcttgaaa
     7081 gaacttttgg atgaaaaatt tagccggaag atagtagcat cccatattac atcaagtaac
     7141 tataggggca attcaaaaaa ttatcgcccg gaaaatttga ttgactcaga ttatgatacc
     7201 ttttggtcaa ccgatgaggt ggagattaat gccactatcg aaattggatt tgaagatttg
     7261 actgaaatta attttattgt gataaaagaa acaattcaac ttggacagcg aataaaaaag
     7321 tttacaatag aagcaaaggt tggtgctgac tggaaaaagg ttgcagaggg tacaacaatt
     7381 ggatataaga gaatcctgcc tttagccggt attcaaacaa ataaactaag aataaaagtg
     7441 gttgatgcaa aagcctgccc ggtaatatcc gaaattgaaa tatattaaat cttattatgg
     7501 atgatttaag aaaaaaaatt caaacggatc cttatttgaa tttccttggt atagaattag
     7561 aggagttgag cgatgggtat gcaatttgtt cattatcatt atccaaagat atggtgaatt
     7621 ttatgggcgt tccacatggt ggtcttttgt tttcactggc cgatgcagct ttgtctgcag
     7681 cgtcaaattc cgaccactac ccatcttacg ccatagaggt aaatggatca tttctggcga
     7741 aggtttctat aggtgatacg ttgactgcca aatcggtgag aattcaccaa acccataaaa
     7801 cggctgttta caaaatggaa atttttcatt tggaaaaaat catcgcagtt ttcagaggta
     7861 cagtttatcg aagcaaaaaa tgaagttgtt tctcctcagc ggattgggag ccgactcacg
     7921 tgtttttgat ttcctggacc ttccaggaat cgaactgaaa ccggttgaat ggctaatgcc
     7981 attaccgaag gaacgaattt ccgattatgc cggaagactt tctgaagctc tgaagcctta
     8041 tgagccggtg aatcttcttg gtgtatcgtt tggtgggata attgcccagg agatggcaac
     8101 gcatctggat acccggaaag tttttattat ctcaagcata aaaagttctg cggaggttgg
     8161 tttagccttt catataacca aaagacttgg aattcttaga ttaattagcc caaaaaaaat
     8221 gaagcaaagc atgatgaaaa tgggaccgtg gctttttggt ctggaaaagg gatcggaagt
     8281 aaagttattt aggaacatta ccacggatac taatgaagag tttctgaaat gggcggtttg
     8341 tcaattgcat ggttggaaaa catcgtcttc aaatcttgat gtaattcata tccacgggat
     8401 taaggacaag atgtttccca taaataaaat aaagaatttt attcctattg aagatggcgg
     8461 ccatttcatg attgtaaaca gagctgaaga gatcagtaaa attattttgg aaaagataaa
     8521 atagcccact aaccgtgggc ttgcactgtt agctggctga tttagcaccc atccccagaa
     8581 ccgcgccaat tactcctcca ccgatggcgg tcattaccgt aaaagcaata acatctacaa
     8641 atgctgcggt aagattcatg atattggatg tatcatacat aaccatatca aaagacaatc
     8701 cgatcataaa cccaagaata cctgctgcct ttaatccgtc tccaaaagag gtgatgttag
     8761 cccactttaa aaagatatag gaaaataatg ccgccagtga taaattacct aaaaccagtg
     8821 gccaccattc catttcggtt ttagttactc caattgcggt tcccgcattg ttgataaaga
     8881 agtcttccaa tagaactgca taaattagaa acccgagaaa gaagtatgct actccaccaa
     8941 caattgttcc tttgatgaat tttgatgtat ccatagtatt tttgtttgtt ttgatttaac
     9001 tgacagaaat ttagatttaa aattagataa ttcaatagca aattgacggt ttctaagtga
     9061 acctgctttg agaaggcctc tttgcaattg aaattgctca aattaagaca taatagagtc
     9121 ctatagttct tttcattaaa gcagccgatc acttaaattt acaactttga cggccagttc
     9181 atctttatgc gttgagccaa attcaaatgg acacttaaca ataatcatta aaaaacaacc
     9241 aaatcactcc ctaaagcaga atttactgat aatttccttt taagaatcga gatttcaagg
     9301 gtaaaattgc agtcctgttt aaagtgggga aaaactaatt agacacacaa ccaataaaac
     9361 tcaagaaaaa gagaatatga aaagaagaga gcctttggcg ataattggaa ttggctgcaa
     9421 attccctgga gatataaatt cacctgatga tttttggaaa ctgttgatag agaaaagaga
     9481 tgctctcacg agaattcctg ccgacaggtg ggatgccgac ggtttatatg cgccagaatt
     9541 cagaagagca gggaagataa gtgtaaatcg cggtggcttt gtcagggatg ttgacaagtt
     9601 cgatgctggc tttttcggaa tcagccccat ggaagccagt cgcatggatc cgcaacaacg
     9661 tatgctcctt gaggtctctt acgaagcggt gcaggatgca ggcatcagat taaacgattt
     9721 ggaaggaaag aaaatagcgg tagtaatagg aatctccgct catgattacg gtgacattca
     9781 aaactcaccc tctgaaagag ttaacatagg ggcccacacg aatttagggt ctgcattgtg
     9841 cattaccgca aatcgaatct cgtattgcta taacttaaag ggtccgagct ttgcaattga
     9901 tacagcctgt tcatcgtcat tgaatgcgat tcatatggct tgccgctcaa tctgggatgg
     9961 cgacgccgaa ggggcatttg caggtggtgt gaattcaatt cttaaacctg aaccccaaat
    10021 gggttttagc aagggaggat ttttatctcc aaatggaact tgcttttcat tcgacgatcg
    10081 cggtgatgga tatataagaa gtgaaggagc aggtttagtg tttataaaac ccctggttca
    10141 ggccgaagca gataacgatc ccatatatgc ggtcattaga ggtacggcaa taaatcagga
    10201 cggagcgacc aaaggaattt cagttccgaa tccgatagca cagcaagagc ttttagtgga
    10261 agcctataaa gatgcaggtg ttagcccgca tgaagttacc tttgtggaag cccatggaac
    10321 cggaactttc gtcggagatc cgatcgaaac aaattcaata ggaaaagtga tcggcgggaa
    10381 acgaaaaact gtttgttata tcggttctgt taaatcaaac atcggacacc ttgaacctgc
    10441 atccggaatt gctggtctgg ccaagatggc gcttgcactc aaaaatgaaa cgatccctcc
    10501 aaacatccac tttaaaaaag gcaatccaaa tattccattt gatgatatga tgttgaaggt
    10561 tcccacagaa ccaataaagt ggaaagataa tggcaaacca aggtacggcg gagtaaactc
    10621 attcggattt ggaggttcaa atgctcacgt aattctggaa agtataaggc aaaatatcca
    10681 gaagaacgat aagccaggat tacaaattgc aacaatatcc gcacgtagtc aggacgcgtt
    10741 aactgctctg acaaaagact ttatcgccta ccttaaagat aagaagaaca aagcaagtct
    10801 tagtgatata tgctattcaa cagctgttcg ctccacgcac catgatatca ggctcgccgt
    10861 tgtagcagaa tcaaagaagg aacttgcccg gaatctgaaa atctttctgg caggggaatc
    10921 ccttactgaa attgctgaag gtcaggcgct cgaaataaaa gacaaaattg cgttcgtttt
    10981 ctcagggcaa gggccacaat ggtggggaat gggccgtcaa ctttttgaga atgaacctct
    11041 ttttcgcgag accataatta agttggataa gatgcttggt aagcacgcca gctggtctct
    11101 tattgaagag cttacaaaag atgagaaaac ttcccggata agcgaaacaa acattgcaca
    11161 accggctata ttctcaatcc agattgcgct ttatgaaatg tggaaagctt tgggcatcac
    11221 cccctcagcg gttgtaggac atagcatcgg tgaggttgca gccggttatg cttcaggagc
    11281 attaactctt gagcaagctg taaaattgat ttttcaccga agccgcgtgc aacataaagc
    11341 aaccgacaaa ggcagaatgc ttgctgtagg tttacccctt gaagaagcag taaaattaat
    11401 caatggtaat gaaggaagag tttcagttgg tgccgtaaac ggaccttcaa tggtagtgct
    11461 ttcaggagat acagatgtta ttgaagaaat tgccgaagtc ctcgataaga aagatgtttt
    11521 tcaacgtctg ctggctgtaa atgtaccctt ccacagccac cacatggagc cattgaaaaa
    11581 tgaattaatt acctcccttg gttcattcaa aactacttca actcaagttc cattttactc
    11641 gacggttgaa ggtaaaaaag tgaaaggtga agatcttaat ccaacgtatt ggtttcgaaa
    11701 tgttcgggag ccggtttatt tcacttctgc aattgaatct ctaatcaatg acggatttga
    11761 tacctttatt gaacttggcc cccaccctat acatgcaatt ggcattaatg atctgattag
    11821 tcaaaagaaa tctaaagctt tagtcgtacc ttctttacga aggaatgaga atgaaaagag
    11881 aacctttctt tcttcagtag cggcattgca tacatggggt tgccagattg actggaaatt
    11941 attttacggt aatgataatg gctttgttaa acttccccgg tacccctggc agaaggaatc
    12001 tttttggtta gaaaccgaag aaggaaaacg attaaggttg ggcaccattg aacatcatcc
    12061 tcacctggaa agaaaaacct ccagcccaag agaaagccat aatatcctgt ggaatgtaaa
    12121 tctggataag agatcttatc catatatcga agatcacaaa gtacagggac ctatagtatt
    12181 tccaggagca ggtcatgtag atcttgtgct gggagctgca atggcatcat ttggtgataa
    12241 atttatttct ctggaagaca tcaatttcga aaatgctttg tttttacctg actccggtga
    12301 gccacctgaa attcaaattg acatttccca cgacgcgggc gattattata tctatacaaa
    12361 accccgcggt atagaagccc catggaccat gtgctccaat ggcaaaatga atcatacagg
    12421 agagaaattc aaatcaatcc cgattaacct gaatgaagtc cgtaaaagaa caataatacc
    12481 tgttccggtt aaagcaatgc atgatgaatt acttgaaagc ggcttatatc tgggtccaag
    12541 tttccgcgcc atcaaaaagt tgtggagaag tgatgggcac tgggaatctt taagtgaaat
    12601 tgaagtgcat gaaagcattc gatctgaatt tctccagttc aatatacatc ccggcgttct
    12661 tgatagctgc ttccagaccg tgtttggaat ctttaatgaa cgggaagatg ttaacaagaa
    12721 gatgggcgta tatgtacctc gtcatatcga caaaataaaa ttttatacaa agccaaattc
    12781 gttcaaattg gttgtccacg gtagattacg cgagtggact gatgagtatg ctttgggaga
    12841 actttggatt ttcaacaccg atggcagttt ggttgccgaa ttccatggat tcctttcaca
    12901 atatcttaaa gggtcaagag gagaaaccgg cagtgagcag gaaaaatggt tttatgaata
    12961 caattgggtg atgaagccaa gagcagatca ggaactgctt aggaatcccg gtaattattt
    13021 ccaatccccg actaaaatta agagtaaagt aaaagaaact ataaaaaaag ttgttacacg
    13081 gccggagcaa agagaatatt ataagcacta tgagccggaa caatatgccc tcacgatcga
    13141 atatatctgc acatccctga gggaattggg tttttccttc aaaaccggac aaaaagcgga
    13201 ccttaaatat ttaattgata cttataaaat tattccacaa catttccgcc ttttccatca
    13261 catgtttaat cttctgaaag atgccggaat tgtaaaagga aaggatggtc attatgaaat
    13321 tgtcaagaat ccgaaagaac gggatgtgaa tagctggatg gatgatctgc aaaagaaata
    13381 tccacagttt catcatgaat ccaccctgct tggcagatgt ggcccacaca tcacgggggt
    13441 tttgcaagga acagttgacc ccatcgaatt gatcttccct gaagataaat gggatgcaat
    13501 tgtaaaatat tatgtggaag gatttgcttt caggaaatac aatgaaatgg ccgcgagggt
    13561 tgtcaaagaa cttttagcca ggcttcctaa agatcaaact ctgagaatac tggaagtagg
    13621 tgccggcacc ggcggtatga ctcaggcaat acttcctcta ctccccgcgg atcgtacgga
    13681 atacattttc actgaccttt ctcatatgtt tatgctaaag gcgcagcaaa gattttctaa
    13741 ctatccattt gttcaataca agattatgga tatcgagaat gatccggttg aacaaggatt
    13801 cacccaaaat tcctatgacc tgataatagc atccgatgta attcatgcta ccagaaacct
    13861 gggaatttct cttggcaatg tgaaaaagct gcttgcctct gaaggagtcc taatgatgct
    13921 ggaagtaaca aattctcccg tttacctgga ttttattttc ggcatgacag aaggttggtg
    13981 gttatttgaa gataccgata tccggccaac gcatgcaacg atggatcctg ctcgctggaa
    14041 aatggtcctc gaaaaaaata agtttaacga tgtggctatt tacagtgact tcgaaaggaa
    14101 tgattcctca tgtcagagcg ttatagtggc ccgctctccc aggattgatc tttccaaatt
    14161 agtgaaagaa aatcccggaa aaaaagccag agctaattgg cttgtctttg cagatgataa
    14221 aggagtttct gataagatta tgaatcatct taagaagcta aataagaatt gtatagttat
    14281 acaccggggt ggtgattttt ctgaaagtac taaaaatgaa tttactatcg atagtcttga
    14341 tcaggaaggt gctgacaaac taatcgaata tatctcggat gaagacaatt tccagggctt
    14401 gatttttgcc tggggattag attctgctga taacaataag ctatctgaat caactatcat
    14461 caaagctgaa gaagattcat cagcgatgat gatgaatata atgagaaagc ttaacaaaat
    14521 gaactatgag aaaaatccgg atatctgggt actactacga ggtacgcagg tggtgggaga
    14581 ttctccaaca acaataaatc tttctcagca aggacttcgg ggagtcagcc gggtaatagt
    14641 aaatgaattc ccaaattaca aaacctcgct tgtagacttc agttcacaag taaaagagga
    14701 ggaaattcaa tcttttattg atgaattatt taatgatgag catgttgatg aaattgcatt
    14761 ccgtggaaaa aaaagatatg taaaccgcct cgaaaggcta tcaacaagaa aaatagcaaa
    14821 cttatcacgg aaaaccgtta acgctgaagg aactccatat gttgcaggta tttcagaata
    14881 tggagtcctt gataatatca gcctgcgtca aacctccctg agaaagccgg aagaggatga
    14941 agtggagatt agagtaaaag catctgcttt gaatttcagg gatatcatga tagcaatggg
    15001 gctcctgtca gatgccgcgg ttgaaggcgg attatttgga agaacatttg gattggaatg
    15061 cgcgggtgtt gtgagcgcgg taggaaaaaa ggttaaaagt gtaaaagtgg gtgatgctgt
    15121 tatggctact gccccttcct gtattgccgg gtttgcatat ccgaaaggtt ctcacgtagt
    15181 taagaagcca aaaaatatta gctggactga agccgcaagt ttgcccgttg tctatacaac
    15241 tgcatacttc tccctggttt atcattgcag gcttcaaaaa ggtgaaaaaa tattgatcca
    15301 tgcagctgcc ggtggtgttg gcatagctgc cattcatatt gccaaagcaa tcggagctga
    15361 aatctatgct acagtaagca gtccggataa gaaagcttat atccggaaaa ttggagtgaa
    15421 atctgagaat ataatgaatt cccgtacgct tgatttcgct gacgaaatta tggagaaaac
    15481 cggaggccag ggagttgatg ttgtgcttaa ttccctatcc ggtgaagcta tttataaaag
    15541 tgttaaatgc ctgagtcctt atggcagatt tgttgaaata gggaaaaccg atatctaccg
    15601 gaatagcaaa ctgggtttgc agccatttgg aaataacctt acctattttg gcgtggacgt
    15661 cgacagatta ttcaaacaaa aagaaaaatt tggtggcaaa ttattccagg attcaattga
    15721 ttatttcgta aagaataaat tcccggttca tccggtaaaa gttttttcca ttgacaacct
    15781 tgctgacgcc ttccaacaca tggccggcgc aagacacatt ggaaaaataa ttattgaaat
    15841 gaaaggtgaa gtgcaaatct cacctcccag ggaaatagcc tttgatgcaa acggttctta
    15901 cttgttaacc gggggcgctt ccggatttgc actttcagtt gcaaactgga tgacgaccaa
    15961 aggtgcacga catcttgttc ttatgagccg cagtggaccc aaaaccaatt ctgaaaagga
    16021 aattgtagcc aggatgaaga agcgaggtgt gaaagtaatg attgccaaag gtgatgtctc
    16081 caataaaaag gacgttgata gaatttttgc ggaaattaaa aaggaaatgc ccccgttaaa
    16141 gggaattcaa catgcggcaa tggttctgga cgacggcgga atacccgaga ttgatcatct
    16201 gcgatacttg aaagtgttta agcctaaagc tataggatgc tggcatttac acaacgccag
    16261 caaggcatta aaacttgacc attttatttc ctattcttcc atatccgcga tctatggaaa
    16321 ccctgggcag gtgagttacg tcggtggaaa cagtttcctc gataattttt catcatacag
    16381 acgttctttg ggtctgcctg caacaactgt aaattgggga gttattggtg acgtaggttt
    16441 tgttgcgcgc agtggtaaag ttggaggttt gctgtataaa caaggctgga aagccttcac
    16501 gctcgatcaa gctttgggaa tattagaaca aatgcttctg aataacccca tacaacgcgt
    16561 tgctaccgac tcggattggg aaatgattgg aaacttttat ccccattcag ctacatcaag
    16621 caggttcgcc catcttgtaa aagagaaaga gttaggcggc gctggcggag ttggtcaggg
    16681 tgattgtgca ctcaaagcaa ctttgcttga atcaagtagc gaagaaaaac cggagatatt
    16741 gctaaccgcg cttaaagaca cattcgcgag agtaatagga accacagcag ataaagtaga
    16801 gacttctcaa cctgttacca aatatggtct tgattctctg atggcgaatc aaattcgaaa
    16861 ctggatccaa agtaacattg ccgtagatta ttccatgatg cgaattatgc gtgggccaac
    16921 aatggaagaa atgaccgctc aaattctgga tgaattgaca ggccagaccg gctccagtaa
    16981 tggactattg gaagaacgga ccgaattgga taaatggata attagaacca agcgggttga
    17041 aaacccaaga ctccggttat tctgcctgcc atattttgca ggaggcgctt caattttctc
    17101 aagctggcac gagtttctac cggaagatat tgaagtctgc gccattcagt ttcccggccg
    17161 tgaagaacga ggtgatgaaa aagcttatga tgacgtgttt gccctggtga aaaaactggc
    17221 agaagtcata gaaccgttac ttacagcgcc tatagctttt tacagtcaca gttccggagc
    17281 tggtattgcc cttgagttag cccggcattt gcgaaaaacc aatgatgtaa atcccgtcaa
    17341 attcattgtt ggcggatggc gggctcctca cctgagcagc ccattcaaat ttctgaatgc
    17401 aattaaagag gatgaagttt ataaggagaa aaatattcct aacataaaga atcatttgcg
    17461 ctcattggaa attccagatt ctgtaattga taataaggaa gtgtttggtg aaatgcttcc
    17521 ttcactcaga gcggatatcc ttttaggaaa gaagtataaa tactatgagg atgagccgct
    17581 gccttgtcca ctggttggag tcgccggtac aaaagatacc gtatttaccg aagaccagat
    17641 aaaagcctgg tcaaaacata cttcttctga atttacattc aagaaaatca atgggagcca
    17701 tcttttttgc cgggacaata aagaggaatt attggagttc cttagccacg aattagtgga
    17761 caatgtaatt gcgtagactg atgaaatctg taaaagaagt catcacctcc ccttttgaac
    17821 tgctccggaa gtttaattca gcgattggac agggagaagt tcatctttat gcctgcaagc
    17881 tggatcccgg tagcgagaga attaattacc tcgaaaccct gctttctgaa caggagaaaa
    17941 agaatgtttc cttttttagt tttgagaaag accagattgc ttataccgca agccagggat
    18001 ttctacggct ggtagttgct ttttatctcg atatagacgg cagtgaagtt aaatttgcaa
    18061 aacacagaaa ggggaagcca tacctgttaa atgacacatc tcttttatac aatatttcta
    18121 actcaggaga ttatgtgatt atcggtattt cacggcatgg agagattgga atagaccttg
    18181 agaagattcg acccctggat gactttgaag atctggttca taagaacttt actcccagag
    18241 aaagagaata tattggtaaa gttcccaccg aaactataaa caggttcttc aaattatgga
    18301 cccttaaaga ggctttcctg aaagcaattg gcgaagggat gagactaaca ccggataacc
    18361 ttgagttttt gattgaaaac ggtaaattta aattgcacac gggaccagga ttttatgaat
    18421 tcgacgactg gcattttagt gatatcacta ttggaaataa ttaccttggg tcattagctc
    18481 atatgaagaa cccggtttat gtttctgaaa taaggtatct atgaattatg ccgtaacgag
    18541 aacctttgat ctcttaaatc aatatcagga aaaattccct gataaacctg acgccctcgc
    18601 aggaattgaa aacaaaaagt gggtcacctt tagttccaaa gaatatattg aaaattccaa
    18661 cttgctcagc tatggccttt tggcgcttgg aattaagaag ggtgatgttg ttgccactat
    18721 aaccaataac agacctgaat ggaattttgt ggatatggga atcgctcaag ttggagctat
    18781 ccatttgcct attttcacca ccctggattc agaaggttat aaacatattc tcacacaatc
    18841 acgacccaaa gttattttcc tatctaatgc ctctctggct gtaaaaattg aatttctcaa
    18901 aaatgacttg cctgaaacaa gtttattcac ttttgataaa gaagagaatt ttgatcattg
    18961 gttgaaaata atcgaaagcg gaaaacagaa tgaagctatc cttaaagatc aggttgaatc
    19021 gataaagaaa gaaatcttac caaaggattg ggttactctc ttatatactt cgggaacaac
    19081 tgataattca aaaggagtga tgctctcaca tgaaaatctt atgactaatg ctgctgctgc
    19141 cgctgaagtg tttaatctaa agccagagca taaatatctt agttttttac ccttatgcca
    19201 tgtgggtgaa agaatggcga attaccaaac tcaacttaca ggctgtagct tatattatgc
    19261 ggaaaatata ggaacaatag ccagagatct aaaggacttg aagccaaatg gcttcggtgc
    19321 cgttccccgg atccttgaga aagtttatga caggataatg gaaaaagcgg agaaactgac
    19381 aggcttgaag agatggcttt tcttttgggc catgcgttta gggtataaat acgatccatc
    19441 tgtcacaaag ggtttcggat acaactgcca gctatggttg gcaaataaac tggtcttttc
    19501 aaaatggcgt gaagcgctgg ggggcaatgt tcagtttata ggagttggtg gggcaatgct
    19561 gcaggaaaaa atcgagagag tcttctgggc agcgggaatt aagttactta atatgtacgg
    19621 tttaaccgaa acatctccca taataactat caaccgggca aaaggaccat tactaaaggt
    19681 aggaacagtt ggagccccca ttaaggatgt tgaggttaaa attgttgatg atggtgagat
    19741 tgtatgccgg ggtcctaatg ttatgttagg ttattataat aatgaaactg caacaaaaga
    19801 agtaattgat tccgatggct ggtttcatac cggagacatt ggtatttttg aagatggtaa
    19861 attcctcaga attacagaca ggaaaaagga gatattcaaa ctctccaatg gcaaatatgt
    19921 acagccacaa acaatagaaa ataagttgaa agaatctcct ttcattgatc agtgtatggc
    19981 ccttggtgtt ggtgagaaat ttgccagcgc tttaatttct ccaaataagg aagcattgaa
    20041 gagttgggct aagactaaca atattaacga aagtaatgtt caaactctgg tacgattaaa
    20101 accggtgatt gagttttata aaacaattgt tgatgaggca aataattcct ttgacaaaga
    20161 tgaaaaactg agtcgaacga aacttattgg tgatgaatgg actcctgata ccggagaact
    20221 ctctcctacc ctgaagctca agcgtcgtgt aattactgaa aagtatagaa atttgatcaa
    20281 tgaaatcttc ctgaaggacg aaagattccg gtttaagtta tgaaaattgt tgaagtacaa
    20341 aacaataaac acgaaaatca gtttctccag gtagccaggg aattatacaa aatggatccc
    20401 aattggatct gcccgctgga taatgatatc aggagtgttt ttaatccgct tgaaaattcc
    20461 tatttcaatc atggccaggc taccaggttt atccttgaaa ataataacaa tgttatagga
    20521 agaatagcgg cattcattga taagaaaacc gctgataatt cagaattgcc tgccggcggg
    20581 tgtggtttct ttgaatgcgt gaatgatcag gccgcggcag atctcttatt caattcagca
    20641 aaagaatggt tgctgcaaag gggcatgcaa gccatggatg gaccaataaa ttttggagaa
    20701 accaacaaat attggggatt gctggtggat ggtttctcac aacctgctta tcaaattgca
    20761 tacaatcacc cctactacca gaaattattc gaagactttg gattcaaaat ttactataag
    20821 caggaaggat tccattttta tctggatcgg gaaataccac ccagattctg gaaaattgca
    20881 agttgggttg caaaaaaacc ggattacagc tttgagtatt tgcaattctc caaagtagag
    20941 aagtacgtaa aagattttac aaaggttttt aaccaggcat gggcagattt caaagaagat
    21001 tttgaacccc tggaagaagc gtatgttcgt aattttatta tgaaagcgaa ggttatagcg
    21061 gaagaaaaat ttatctggtt tgcctacaac aaaggagagc caattgcagt ttacctgatg
    21121 atccctgacg taaaccagat ttttaaaggc ttcaagggaa aactaaacct gctaaataaa
    21181 ttgaaagtgt tgtacggaat taaaacaaaa aaaataaccc gtgcaagagg tatcctcatg
    21241 ggagttatac ccagtttcca gggtttgggc atcgagtctg cctttattta ccacctgaat
    21301 gaagtcttta agaaaatgcc acattacaaa gagatagaat tttcatgggt tggagacttt
    21361 aatcctgcaa tgcgtaaatt atggctttcc gtgggagccg aacccaccaa acaatatatc
    21421 acatatcgat atttatttaa ccgtgaagct gaattcaacc gttaccccat tccggttgaa
    21481 aaataaattc agatttaatt gcattagctg aaaataagtt aaaatattct cgctatcttc
    21541 cttctaacct gaataaaatt aactatgcct tttgatcaaa accttgcaga tagaatccac
    21601 actatcctga attcaagaaa aataaaagcg gtggaaaaga atatgatggg tggtcttaca
    21661 tttatggtga atgataaaat gtgtgttgga attgtgaaag aggaccttat ggcaagagtg
    21721 ggcccatatc attatggtaa agcacttaaa atgaaaggtg ctcgcgaaat ggattttacc
    21781 ggcaaaccta tgaaaggatt cgtatttgtt gccccggatg gaattgattt ggatgaagat
    21841 ctggagaaat atcttcctat ggcacttgat tataacaagg aagcaaaaag ttcaaagaga
    21901 aaaaaaatta gatgaacttg aacttcagga aattaattgg aaattacttc ctcccctatg
    21961 aacctgttgg cggtcattcc ccatttttcc ccaaggaaaa taatttctcg tgctaacgag
    22021 agcgacaccg aaagattaat ctacataaat ttgtattggt tcattgaaag acccttgatt
    22081 ttgaaatgaa aataaaaacg ttatcagaat gcgaaaaaga agggatacat tctaactcta
    22141 atattttgac aaaaacgact aaaaaaaatt gtattttgac accgtaaatg gcagataaag
    22201 cattcataac accaaaagtt ctaaaatggg caagagaatc tgcaagaatg accgaagaaa
    22261 ccgctgctgc taaagtttct gtgaccattg aaaaggtaaa agaatgggaa gcaggggcaa
    22321 gccagccgac tattagacaa gcacaaacat tagccaaagc atataaaaga ccttttgcct
    22381 tgttttttct gccagaaatt cctcgtgact ttcaaccatt acaagacttt agaaaatcag
    22441 gttcaaaagc ttttacaact tcttcagttt tcataattcg agaaattcaa caaaagcaaa
    22501 cttggattag tgatgtgtat tcagaaaatc aagaagaaaa attacctttt gtagggcgct
    22561 tttctctaaa tgacaatccg caaaaagtag ctcaagacat cctatcaaca ttaaaaataa
    22621 atccggcgac ttacaaatca gacaatccaa taaaggaatg gatcgatgca gcggaaacaa
    22681 acggaatttt cgtttcaagg acaagcttta tacactcacg acttaaatta gattcagaag
    22741 aattgcaagg ttttgcaatt gcagatcctc atgcaccatt catttttgtt aattctgacg
    22801 attggaatgc accacaactt tttacactag ttcatgaatt agctcatatt tggattgcgg
    22861 aaaccggaat ttcaaacgag attgaacctg acttaaaaca caaagacaaa tttcatactg
    22921 ttgaattatt ttgcaatgga gtggcagcaa atgctctaat gccgaaagaa attgtattaa
    22981 gctttgactc ttcatcattt caaacttcaa aagacgtttt caaagttgcc aatcaattgg
    23041 gtgtaagttc atttgcatta ttagttagag tcttaaatct taatattatt tcaactcctg
    23101 cttatcagaa actaaaaaga caagcagata ttgattatgc tgactattta agaagaaaag
    23161 cagaaaagaa agctagacaa aaggagaaag aaaaaccagg tggaccaaat tattttttac
    23221 ttcaattaaa tagaaacagt aggttattta ctcaaactgt gttagacgca tttcggggtg
    23281 gttttatcga gccgacattg gcgagtaacc ttttaaatgt tcaggtaaat aaatttcaga
    23341 aactagaatc ccaattgttt agatgagcat aaacgcaaac aaatattgtt tggatgccaa
    23401 tgtattaatc caagcatggc aaaaatacta caatccaaaa ttttgtctcg actattggaa
    23461 catcttgatt gaacttggta aacaagacag gatttttact cctgaattag tatccgagga
    23521 aattacaaga accgaagacg acctttcaaa gtggttaaaa ggaagtaaaa ttcctattaa
    23581 gaaaattagt gagcctgtaa caatttgctt gcaaagaatt tattcaacta acccaattca
    23641 tcagaactta gttgacaata caaaagcccg ttctttggct gacccttggt taatagcaca
    23701 cgctttgtat gaaaacgcaa ctgtggttac aaaagaggaa aagataactg ccttaaattc
    23761 tagcagaata aaaatcccaa atgtatgtga caacatggga gtgagatgga taaatgactt
    23821 tcagctcata gacgaattgg aaataaaatt taaatgttcg ttgacttaga tgagacgcaa
    23881 ttatttcatc aattataaaa atatcacctc ccgaattgaa tggacgtttg aagaattctc
    23941 cattacaaag aagttttatc aggtaaaata tttaaaatac cgaagtttga gcgattgccg
    24001 gcctgaatta ataaggtttc cggtttttat ttttttaaga ccctatctgt gcaccgtcag
    24061 ttttcaacag aagtctgttt aattcagctt tcataacttt cagcacttct tctgaacctg
    24121 gggcatcatg gatattattt aattcgtacg ggtcttcagt caaatcatat aattcatcca
    24181 tattttttag atccaaataa cggatgtatt tatatcgctc atttctcacc gccttgtacc
    24241 ccatttttag aattcgtgga aatacggtat cactatagta ttcaatcaaa aaagaacttc
    24301 tccaatcctg agtttttcca ttcatcaggg gaaccattga tcgcccgtgt atatgggctc
    24361 caggtataag tccggccaga tcaagcaagg tcggagcaag atcaatgctc agaatcattc
    24421 tttcattggt ttgcccgggt tcaaagaccc tggggtacct aatcaacata ggaattctta
    24481 tcgcctcttc atatgcaaga cgtctttcaa cactcaagcc gtgctcgcca taccaatacc
    24541 catgatcaga agtaaataca attatagtat tctccaacaa attgctttct tctaattggt
    24601 caatgattct gccaagactt tcatccacgg ccatcagcat ttcagacctt tctcgtattg
    24661 ttttttcatc agtggctgtt tctcttccca attgaggtag tgcatcaatt tgccgcatca
    24721 acgatggctt atctttggga ggtaaatacg cgtttggtct gcgttgaaat actttatcgc
    24781 cgtacattcc tgtgtgacgc tctgcaggaa taaatccgcc ggagccgatc atttcaacac
    24841 ttccatcatc cttttgcttc atatttggat gcagcgcctt atgagacaaa taaagtagaa
    24901 aaggctgaga tcgctgggcg gaaataaact ccgtgctaaa ctcagttaaa acatctgtaa
    24961 catatccttc tatatttttt ttgtctccgt taatatttaa attaggattg attgcctcac
    25021 cctgtccttc cagcgcaacc catgtatcga atccaggccg ttggctatcg tcattcccca
    25081 tgtgccactt gcctataaat gcagtttcat aacccgcgct atccagagta agcggaaaag
    25141 ttttaagcct atgactttgt tcattccggg caaggttatc agttattcca ttcgtatgcg
    25201 catattggcc agtgagaaaa cttgcgcgac ttggagaaca caaaggcgtg gtagtaaaag
    25261 catttctaaa gtagattcct tcccgggcaa ttctatcaat attgggagtt ttaatatagt
    25321 tgtgtcctgc caaaccaaat tcgtcccatc tcatgtcatc aaccagaatc acaacaatat
    25381 ttggcctggt gatttcggat tttgttgctg gatccatgca gcttgaagta gcaatcaaaa
    25441 tcagaattat gtgaaaattt aggttaagct tcaattcggg taaaatcatt ttggatagta
    25501 attttgctaa tgaagaggta aatacctagc gaattttgca aattttaaag gacttatggg
    25561 taacatactc aaataatcaa ttgagaatcc ctgaaaattt taggaatgtg acaaaagtca
    25621 ctgtaaagtt gattttcctc caatatcttt aaaactaatg agcagaggtg ttaaactcgt
    25681 tttagcttgt tttctttgct tggtagtaat taccggactg gtttacatag ttcaatattt
    25741 aattagttag aagatgaaaa aaaatatggg tacggctgat cgaattatca gactgataat
    25801 cgcggcaatt ttaggaggtt tgttttatgc cggtgctatt tctggagcat ttggcattgt
    25861 attgcttgtt ctggctgtaa tctttgctct taccagcatg gtgagttttt gtcccattta
    25921 taaaattctg ggtacaaata cttgcccaac agaaactagt aaggagaagt tataaattct
    25981 gcaagttaag tcccggatat tggtgcaaat ctttaattta gctttatgct gaattttgat
    26041 tttaatccat ttcccattct ggaaactacc cggttttcac tcagacctat ttctgaaatt
    26101 gatattgatg agatttttga gcttagaagt gacccgggag tcaacaaata cctgtcacgc
    26161 aaaacggcaa gggataagaa agaggccctg gaatttatta aatccataat tgcaggaatt
    26221 tttgccggcg aatggattta ttggggaatt tcctttaaag atgaacaatc attaatagga
    26281 acaatttgtc tatggaatat ttcgaaagaa aaaaaagaag ctgaaattgg ctttgaatta
    26341 ttacagtcat ttcagggaaa cggattcatg aaagaaattg cgcccgctgt aacgcggttt
    26401 ggattcgaga aaattcgact actcaccatt aatgctatca cacatcccaa aaataaaagg
    26461 tcaattagaa ttttagaaag cctcggtttt acctatctta agcaggagtc agctaaggaa
    26521 agtctattca tcaaaaaggc tttcaattga aattcaataa aaatcattcc aattcgaata
    26581 atttttgttg tctgattcag caaaaaagtg cattttcata acagaacatc cagaccaatt
    26641 atgaacagcg aaataatgga attattaatg ggtaaccggg agtgggttga aaggacacat
    26701 cagaaatttc cagatttctt tgaaaatctc gctaaaggtc agtccccaaa atttttgtgg
    26761 attggttgtt ccgacagcag ggttcctgct acagaggtta ctaatgcttt acccggatcc
    26821 atttttgtgc aaaggaatat tgggaatata gtggtacaca cagattcaaa tttattaagt
    26881 gttgttcatt acgcgataaa ggttctaaaa gtaaagcaca tcattgtttg cggacattac
    26941 ggctgtggtg gggttttgtc agctatgggt aataatcact ttggctttct ggataactgg
    27001 ctggttcaca taaaagatgt ttaccgctta cataaacctg aactcgatgc aattgaagat
    27061 actaccaaga gaggagatcg gtttgtggaa ttgaatgtga aagagcaggt caataatctc
    27121 tccatggtat cttttatcca ggaagaatgg gaatcgggcg agtatcctta cattcatggc
    27181 tgggtataca gcttaaatga tggactgata agaaacctgg gaatttctat caactcttcg
    27241 gaacatttgg acgaggtgta ccagtattcc gcaatgaaaa ggactgagta atttttgatg
    27301 tcgcattaaa cacatccagg tgggggttta tatttctatt cttcgcggta ttaatgtaag
    27361 tggtcagaag ttaattaaga tggttgacct ggtaaaacat tttcaatcgc ttggcatgaa
    27421 gcaggtcaga acttatatac aaagcggcaa tataattttt caatgctctg attccgctcc
    27481 cggcaagatt gccctaaaga ttgaaaaaaa aatcaaaaaa gagtatggtt ttgacgttcc
    27541 ggtaattgtg aaaagtgcaa aagacatcga aaaaactcta aacaataatc ccttcctgaa
    27601 gcggggtgaa agtagcaacc aattgtatgt aacttttcta tcggatgtgc ccaaaaggga
    27661 actggttaat tctattaatg cgaaacaata tttgccggaa gagtttatct gggataaaga
    27721 tcgaatctac tttttctctc ctcatggata tggaaatgca aagatgaata ataatttctt
    27781 tgaaaataaa ttgaaattga aagctacaac cagaaactgg aaaacggtga ttaagctgtt
    27841 tgagctcgcg gcgcaggctg attaaccatt ctttttgggc cttctacttc aactttacta
    27901 aaatttagtt tttatcccat ttaaaaagcg gtaaattcaa tagtctaaca aaattagcct
    27961 ggtcatgaaa agaatttatt tttacttttt tgggattttg cttatttccg cgtgtgagga
    28021 tgacgataag tattttcaga attttgaaaa tacccgggat gcatctgttc ccgctgttac
    28081 ctcttacgat ggatcgctta aagtaagttt aaatgatgaa aataacattc caataactaa
    28141 gctggatttg agaattcacc tggaatatag taaggcacta agtacaacta ctacgcatta
    28201 ctataaggtt cagggcaccg gactctacag ctgcaattcc tcggtgaatg gagatgcaat
    28261 tatatccgaa ggaatttttt cagtcagaaa atcagatcct gaaagattca ttgcgctgac
    28321 attttctggc ggcaaactgg ctaaccagac cttgcggcta aatctttctg aaattgatgg
    28381 aaatgaaatt tcaggtaaat ggttcaatgt tctcgattca gaaggtggaa ctttatttgg
    28441 aaatacagac tgtcaaagag gattgctatc tctcccaaaa taagaactta aaccaactaa
    28501 aaaaaagccc cacctggtaa tggggctttt ttaattactt aattccgtat ttttctttat
    28561 acttcttata taaggattgt tgcattccat ccagggttat tcttcttccc tgaatgtacg
    28621 cctgatttaa aatgctcgtc ttcatatcca gggcgtctcc ttcagaaaca aataaggtag
    28681 cgtctttccc aaccgcaagt gatccaacct tgtcatcgat cccaagtatt tccgcggtgt
    28741 taagcgtaat catcttaaga gcttcctcct tttctccacc gaaagcagca acagtgcctg
    28801 ccgtaaacat cagatttctt gcactttgcg gtgctgatcc ctgtgaatac ccaaggctca
    28861 cctttatacc ttctttctga agcattgatg gcagccggta tggaaggtca taatctgttc
    28921 ctgaagtgct tggagtatta tgaacttcct caattattac cggaatatta ttttccttga
    28981 tgaaacttgt cacgaacaga gcctggtccg cggtaatcag aacaattttc tgcactcctt
    29041 gctctttcgc aaaacgaatg ctttccacaa tttctttagc attgttactg tgaatgtata
    29101 aggttttggt tccatcgaat aaccctttca tcgcctcaag ttttaaattg ggagctttag
    29161 gggcgaccaa tttcgtatat gaagtggcgt ctctaaacaa tacttccagt tcctgaactt
    29221 gcctggggta gttttcatta tcagtaaaag aaaaagtaaa agtagtaaaa tcaaaattcc
    29281 ctctgcgttt gccgggccag ttcaaatgga ttccttcatc taatttgtag gcagcatcct
    29341 cccaattcca tgcatctaat tgtacaatgg aacttccacc tggtatcgtt cctccctggg
    29401 gcgtaatttg tgccattaat ataccattga acctgtaagt gggtatcatt tctgaatcag
    29461 cattgtaagc aacgattgat cttacattcg gattataatc tccctcttca ttcagatcat
    29521 tcgtagatcg aacattacct acttcaccca ggccaagtga cgaattggga ataataaacc
    29581 cggggtaaac atgcttgcca ctaacatcta ttacttcata gccggtttca tcaatccgga
    29641 cagctgttgc atcagcaaca atagttaact tgccattttc aaatccaata gcagaatttt
    29701 ggataacctg cccattaccc aaatgcgctg tcgccccctt aagcaatacc ggcctgctct
    29761 gggcagccgc gggaatcaaa gggtattgag cgtatgacat tgtgcataca gccatcaata
    29821 gaattgtaat tatgtttctt gttttcatga tattcatttc ctcaggttat ttattttcaa
    29881 acaaatattc gtagccggta aaatcttcgc aatgaaaatc tctttgaggt ctgctaaaag
    29941 gtctggcggt tctggcaccg ccggcttttg catctttcat tttagcgatc agccttgccc
    30001 gctccgcttc aatccacttt ctcatttcca tatctttctc tacatcgaaa tacaccacac
    30061 cgtcaacaat ggttttcaat ggttttgcat aaatactcag cggattctca ttccataaaa
    30121 caacatcagc atcttttcca acttttacac ttcccattct attatccaga tgtaaaagtt
    30181 tagcagggtt tagagtaacc attttccagg cttcttcttc agacatacct gcatacttca
    30241 cagattttgc ggcttcctgg tttagtcttc gaatcatttc accatcatca gaatttattg
    30301 ccaccgttac tccggcaccg gccatcaaag cggggttata tggaatagca tatcttactt
    30361 cccatttata tgcccaccaa tcagcaaacg ttgagccgcc ggcaccatgc gcggccatct
    30421 tatcggcaac tttgtaaccc tccaaaatgt gggtgaacgt gtttacatta aaattaaatt
    30481 tcccggctac tttcatcatc atatttattt cagattgtac ataagagtga caactgataa
    30541 acctttgctt gttgatgatt tccgccatag tttccattgc taaatcccgg cgtggtttca
    30601 ctactcctga cttattcttc agcgcattgt acgctttcat ttgtttatca tagtctcttg
    30661 cggcagtaaa tgcgtccatg taaacctgct ccactcccat tctggtttgc gggaagcgaa
    30721 tcgattgtgc atttgttgat cgcttcacat tttcacctaa agcaaacttt ataaattcgt
    30781 cagcattctc tatcagcatt tcctgaggag attttcccca tcgaaatttc actaacccgc
    30841 tttgaccacc tattgggttt gccgagccat gcaaaatctg tgctgcggta acaccaccgg
    30901 caagagccct gtatattccg atatcttcag agtcaattac atctttcatg cgaaccattc
    30961 cggaatttga gactacgtca ttaattccat cattcgcaag gtgagaatgt tcatcgataa
    31021 ttccggaagt caaatgttta ccggttccat caactatttt tgcaccggcc actgtaagat
    31081 ccttaccaat cctggcaatt ttgccatttt gtaataatac atccgtattc tctaaaatgc
    31141 catccgactc attggtccaa acagttgcat tcttaataag cagcgtttca actaccggta
    31201 ctgtttcatt accataagcc atgaaagggt aaacgacttt ccctaaatct gacggggtat
    31261 catcagagga attatctttc ctgttatttc ctccggaagc ggcagatgca aaggtagccg
    31321 accaatctat ccagtttcca ttcacaagct gtcctttgcc attccaacct gaaccttgtt
    31381 gtccgggagt accggagaac catcctgaaa ggcgtaccat ccccttcgca tctttgtccg
    31441 gtttgtacaa taaggtaatc agatctccgt caattttagc atccacgcta atgtttgtac
    31501 tgtcattaac ttttatcttg aaggtatttt tacccgcttc acctgaaacc tctaaactct
    31561 gtgaagaatt atttacagac aatgtataat tcgcgctata atctttatca tctgttttta
    31621 gtacaaatgg ttttccctgg atccagtttt caaagattat cgcatcatcg ctaaatatat
    31681 tcccattcgt gattatgaaa ttggcatatt taccggctgc aaggcttcct actttatcat
    31741 ataccctcag caagttggcc ggagtaaacg tcattgcttt aagagctcct tgttcgctca
    31801 ggccatattt aacagctttt ctcaggttcg tccaaaaatt attttttgct cttaagtcat
    31861 cagtggtgag agcaaaatta attcccgctt tttctaatgc agccggattc gccggggcaa
    31921 gttcccaatg ttttaagtct tccagtgaaa ctctcaatgc atcaaccgga tcatcaacat
    31981 caactgcttc aggaaaatta atgggcaata taatcgaagc atttgttgct ttgatttcgt
    32041 taatcctacg gtattcatct ccattacctt ttatgatata ttgagttccg aattcatcac
    32101 ctaatttgtc agctctgagt atttccttcc agtttgaaac ttcaaataat tgggggtatc
    32161 gctgagttgt caaccatgca tccagtgatt tatccgtaaa agttttatta ggaagactgt
    32221 tataccattc ggcatcaaaa cgtgtttgcc tcaacacagc tgtaaaaccc attctgctgc
    32281 tggggtattg ctgacttgaa gttcctttgt cgaaggcgta gttggcggcc gccactgaga
    32341 ccagaagttc cttgttggca gtttcttcac ctaaagtaac gaaagctccc gttcctctgg
    32401 ccaacccatc tgctctaaaa gttaaagcgc tgccaaatcc aatatcgcgc aatgattttc
    32461 cagacttgga atcaggacta aattcatctg aggcgttgta ttcagattta atcgcctcat
    32521 ttgcattgta cgctcctttt gtatcagaat taatttgttc acgacctcca aatcctcctc
    32581 cacgccttcg ctccacttcc ggaagtccat aactggtgaa tgggtcgatc attgatggat
    32641 aaatgaactt tcctgccaaa tccacctgga tgtaacctgc aggaatagag aggtttgctc
    32701 caacattttc aatcttacca tcacgtatca ggattgtccc tttgtcgatt ttagtttggt
    32761 aatcgacata tatggttgca tttgtcaggg cgtaagcttt agttcttaca tcctgcacgt
    32821 cattgaccgg aaatgtctcc tgggccagga gatttccctg ccaaaacagc aagccaaata
    32881 gcagtaataa attttttctc atgatttcat taagttagtt aattaaagag gcgaaagtta
    32941 attaaaatct ttagtatcct tggtcgtaat ttgggattcc cgattggaat tttgacgaat
    33001 ggtttatact gattatcaaa gtctgacaat aatataattc gattgcacta aagtgaaaaa
    33061 aatcctcaat tttaggctaa taattatcct gatgctgatt ggcgcatgcc attccaggga
    33121 taaatcgctc aaagaagatt tagcgcttaa ggatacgcta accggcggct ggattacttc
    33181 aaatagcaat gcaaatcccc taggatttgt ttttctaccc aatgggagat tcgaagctga
    33241 aaactttgcc aggggaccaa gtggagtttg gatactccgg tcagattcgt taattatttt
    33301 aatgagaacc gttgagcaag atcaaaagtt ttcttttaag atgaggtttg aaaatggaga
    33361 gttgcttttg aacaactcct cagaccagat gagattttcg aggcggcctt atggagtaaa
    33421 tcctgttttc ctaagtgata gatttgagtc tattaccagg ggaataaatc aaagaaacct
    33481 gaaccaagcc gaaaatatta tggagcttcc agcttccggt aattcagaag gtagtcagat
    33541 tttaggttta tgggagaatg aaaacacccc ggaaaaactt gcagctacag aaccggtaaa
    33601 agacaatgta tttgccggct tctctgaata ctattttatt gaagggcatc tgatttgcaa
    33661 aaaaggaccc atggagaatc ttctttttga gaacaatgaa attgttctgt ggactgactc
    33721 cttgttaaat attttacagc ccaccgaaat tgatttgaaa tctaaagcag aagtagaatt
    33781 agagaaagtc agcagatatt tgaaagcata tggaatccat gaagacaatg gagtattttt
    33841 caggatcgaa gaatccggag tttcagcgcc agattcctga ggtgaggaaa catattagcc
    33901 tgaaaagacc tcatctagct gaacaagtag atttaccact gatttaactt tgttttgaag
    33961 gacactttac tgtcccgtat gaacagaata cgcagcaatc tcctgctgca ggccttagta
    34021 ccgtattaca attttcgcat tcatagaaaa actgacaagc gtcctccggc ataatttctt
    34081 tcttctgatg accgcagacc ggacacgtga ttacagactc aaacgtgatt agctttttca
    34141 tgcttgcttt cttttcaaaa accctttttg ttccgattca tggagtaatt gtgaatgttc
    34201 aatttcatta atagacttta tccttaaacc tatgcgatag ttttttaact gaaatgatgg
    34261 tgtcaggtca gaataaaatc ttgcgaatgg attcccatga ataaaaaaat tcttaagagc
    34321 cagagttttt attgataatt tttgttagta gagtttggaa acattttcag gcggtcttcc
    34381 taatacggct tttctccctt tcaccacaat gggtcgctca atcaaaaccg gattcttaac
    34441 caaggcttca atccattgct tcctggataa ttgctttccc ttaaatttct ccttataaag
    34501 ggtctcgcct ttgcgaacaa tgtcctccgc atccagatct aacttgtcca ggattccctc
    34561 cagttctttt gagtcaggtg tatcttttaa atattgaatt atttcaatgt tcttctccct
    34621 cttttcaagc aaggcgagcg tttgcctgct tttcatgcac ctgggattat ggtatatggt
    34681 aaccatcaag agtgattgtt agagcgtacc gtaactataa tttctcaatc acaaaat
//
LOCUS       DASWHX010000059         5085 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_1899021, whole genome shotgun sequence.
ACCESSION   DASWHX010000059 DASWHX010000000
VERSION     DASWHX010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 5085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5085
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1899021"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2031
                     /locus_tag="VGA21_04290"
     CDS             <1..2031
                     /locus_tag="VGA21_04290"
                     /inference="COORDINATES: protein motif:HMM:NF013717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HEX9649754.1"
                     /translation="DFWMPSYPGSEAAVLLAMAKIILEENLYDRRFVEDWVNWKEYLK
                     KEFSGEAVSFENFIRKLIEVYKEYTPEYAEKESGVKAEMIISISREIGKAGSRFASHN
                     WRAAGSGNLGGWAVARCLHFLNVLTGSVGTEGGTSPNGWNKFKPTFFDNPPAQKFWNE
                     LHFPKEYPLAFFEMSFLLPHFLKDGRGKLDTYFTRVFNPVWTYPDGFSWIEALTDESK
                     VGMHIALTPTWNETAYFADYVLPMGLASERHDINSYETHGGVWIAFRQPVLREVARKE
                     GRKFTHTYEVNPGEVWEEDEFWIELSWRIDPDGSLGIKKHFLSPYRENEKITVDEYYQ
                     FIFENTKGLKEKAATEGLTALDYMRKYGAFEVEKSSYSKHLEKLSEQELRDSQLDPDS
                     GTISKNGKAIGVHVNGKSVTGFPTLSRKQEFFSQTMMDWKWPEYTIPTYIKSHVHPEK
                     LNPENGEYPLIPTFRLPVLIHSRSANAKWLVEIANRNPVWMHNLDARKFGLKNGDLVR
                     ITTDIGYFVDKVWISESIKPGIIACSHHIGRWRRQQDQGNQFGSQTVEIKNLGDGKWK
                     MKKIANVKPFRSSDKDSGRIFWSDGGVHQNITFPVHPDPISGMHCWHQKVKVERAHTE
                     DSYGDIFVDTGKSFAIYKEWLEMTRPAPGPDGLRRPTWFARALRPADETYYV"
     gene            2048..3784
                     /locus_tag="VGA21_04295"
     CDS             2048..3784
                     /locus_tag="VGA21_04295"
                     /inference="COORDINATES: protein motif:HMM:NF013109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HEX9649755.1"
                     /translation="MNKASGIKRIFPFLTWFPLVGRGTLRFDLMAGLTGAVVVLPQAI
                     AFSLIAGLPPIYGLYTAMIIPFVFALFGSSLHMVAGPTTTVSIAMFTSVSLFATRMTE
                     DFIAIALLLTVMVGLIQFILGLVRMGRLVNFISRSVLTGYTAGAAILIASHQLESVTG
                     LKVHPTASFIESWGFMLTHLKDINPYSFGIGIITILIALIFLKISSKLPHLFISMVLS
                     SLAAYFLADSSWQIPLVGYIPSSLPHFEVPANFNFDTINNLLPGAFAVAILGLIEVVT
                     ISRTIAIKSRQSLDSNKEFMGLGLSNMVGGFFSSYAGSGSFTRSALNYELGAKTPMSS
                     IFASLILMAIVLFITPLAEHLPIPTIGGIILIIAFKLIDFKSISQISKASKSEIAVLT
                     ITFLSTLLFDLQFAILVGVFFSLSFYLMRTTTPAVIPIAPNPINTRRKFMNAKVNNLD
                     ECPQLLIVRIDGSLFFGAIEHIKNKLIALRSNSRKRILIVCSGINFVDVAGAEFLARE
                     AESWRSEGGEFYLCSLKKKVRDFLAKGYSERIGMENIYTNKEGAISSIYEKLDRPTCE
                     ACPVKIFLECPQ"
     gene            4095..4634
                     /locus_tag="VGA21_04300"
     CDS             4095..4634
                     /locus_tag="VGA21_04300"
                     /inference="COORDINATES: protein motif:HMM:NF024978.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4136 domain-containing protein"
                     /protein_id="HEX9649756.1"
                     /translation="MKTVGIFLLFIGIMLGCAPVKTKTHFDDKFDFSSYKTFCWLSGC
                     EFVYEGPQRYNDSLAIDKIRHAVIQELLSKGLTQDENNPDILIDFHIIIEEKTNIITH
                     RAQYEDERGYAWYPPSYEVYNYLKGSLIIDMIDNSQSRMVWRSHSVGYLEAFPVITDQ
                     QIERSVREALKDFPPVFNN"
     gene            4802..5083
                     /locus_tag="VGA21_04305"
     CDS             4802..5083
                     /locus_tag="VGA21_04305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649757.1"
                     /translation="MNIARMIKDLKEHYDNPDRKVVLDIVASSRSEWQGSVVPLNEKI
                     NNLSKLIHHLERNYTYVPSKLYDSLVRMNQNYNRIVHRNFKQEYSANVA"
BASE COUNT         1492 a          998 c         1106 g         1489 t
ORIGIN      
        1 gatttctgga tgccttccta tccgggttcc gaagcggctg tgcttctggc aatggcaaaa
       61 ataattctgg aagaaaattt atatgatcgc agattcgtag aagattgggt gaactggaaa
      121 gagtatctga aaaaagagtt ctccggagaa gcagtttcgt tcgagaattt tatcaggaaa
      181 ctgatagagg tttataaaga atacactcct gaatatgcgg aaaaggaatc aggtgtaaaa
      241 gcagagatga ttatttcaat ctccagggaa ataggcaaag caggatcacg gtttgccagt
      301 cataactgga gggcagcagg ttctggaaat ctggggggat gggcagttgc acgttgtctg
      361 cattttctaa atgtgctaac cggcagcgtg ggtaccgaag gaggaacctc cccaaatggc
      421 tggaataaat tcaaacctac gttttttgat aatccaccag cgcaaaaatt ttggaatgag
      481 ttgcatttcc caaaggaata tcctttggct ttctttgaaa tgagttttct gcttccccat
      541 tttttaaaag atggccgtgg aaagcttgac acttacttta caagagtatt taaccctgtc
      601 tggacttacc cagacgggtt cagctggatt gaagcgctga ctgatgagag taaggtgggc
      661 atgcatatcg ccctaacacc aacctggaat gaaacagcgt acttcgccga ttatgtgttg
      721 cccatgggac ttgcttcgga acgacatgat atcaacagtt atgaaacaca tggtggagtc
      781 tggattgcat tcaggcagcc tgtacttagg gaagtagcgc ggaaagaagg gcgtaaattc
      841 acccatactt atgaagtaaa tcccggcgaa gtttgggaag aagatgaatt ttggatcgaa
      901 ttatcgtggc gtatagatcc cgatggcagc cttggtatta aaaaacactt cctttctcct
      961 tacagggaaa atgaaaagat tacggttgac gaatattatc aattcatttt tgagaacacg
     1021 aagggattaa aagaaaaagc ggctaccgaa gggcttacag cattggacta tatgcgtaag
     1081 tatggtgctt tcgaagtgga aaagtcatct tacagtaaac acctggaaaa actttcagaa
     1141 caggaattga gagatagcca actggatcct gattccggta ctatcagtaa gaatggcaaa
     1201 gctattggcg tgcatgttaa cggaaaaagt gttaccggat tcccaacgct ttccaggaaa
     1261 caggagtttt tctctcaaac catgatggac tggaaatggc cggaatatac tatccccact
     1321 tacatcaaaa gtcatgttca tccggaaaag ctaaatccgg aaaatggaga atacccatta
     1381 atccctacct tcaggttgcc ggttttgatt cattcccgat cggctaatgc caaatggctg
     1441 gtagagattg ccaatcgcaa tccggtgtgg atgcacaacc tggatgcacg taagtttggg
     1501 ttgaaaaacg gagacctggt aagaatcacc accgatattg gatacttcgt tgacaaagtt
     1561 tggatatctg aatccatcaa gcctggcatt attgcctgtt cacatcacat tggaagatgg
     1621 cgcaggcagc aggatcaggg aaatcagttc ggcagtcaga ctgtggaaat aaaaaatctc
     1681 ggtgacggta aatggaaaat gaaaaagatt gccaatgtga agcctttcag gagttctgat
     1741 aaggattccg gaagaatttt ctggagcgat ggcggagttc accaaaacat tacttttccg
     1801 gtgcatcccg atccaatcag tggaatgcat tgctggcatc aaaaagtaaa ggtggagcga
     1861 gcccatacgg aagattcgta tggtgacatt tttgtggata cggggaaatc ttttgcaata
     1921 tataaggagt ggttagaaat gacccggccc gctccgggcc cggatggctt acgccggcct
     1981 acctggtttg ccagggctct tcgacccgcc gatgaaactt attatgtata atttcttaaa
     2041 ctgaactttg aacaaagctt ctggaataaa acggattttc ccttttctga cctggttccc
     2101 attagtaggc cggggaacac tcaggtttga tctcatggca ggcttaaccg gggccgtagt
     2161 agttttacca caagctattg ctttttcact tattgcaggg ttacctccta tctacggttt
     2221 gtacaccgcc atgattatcc cctttgtttt tgctttgttt ggatcttcat tgcatatggt
     2281 tgccggacct actacaactg tttccattgc catgtttact tcagtaagtc tttttgctac
     2341 gcgaatgact gaagacttta ttgccatcgc cttattactt acagtcatgg ttggcctgat
     2401 tcaatttatt ctgggcttag tgcggatggg ccggttggta aattttattt ccaggtcagt
     2461 actaaccggt tatacagcgg gagcggcaat tttgatcgct tctcatcaat tggaaagtgt
     2521 gacaggactc aaagtacatc caacagcttc cttcatagaa tcctgggggt ttatgctaac
     2581 acatttaaag gatattaatc cttattcctt tggaataggc attattacta tactaatagc
     2641 attgattttt ctaaagatct catcaaaact tccccatcta tttatctcca tggttttaag
     2701 ttcacttgct gcttattttc ttgccgattc atcctggcaa attccattag ttggatacat
     2761 tccctccagt ttgcctcact ttgaagttcc tgccaatttt aattttgaca ccataaataa
     2821 tttattgcct ggtgcatttg cggttgcgat tctaggctta atagaagtag taacaatttc
     2881 cagaaccata gcaattaaaa gcaggcaatc attggatagt aacaaagaat tcatggggct
     2941 tggattgtct aatatggttg gaggtttttt ctctagctac gcaggatcag gttcttttac
     3001 acggtcagcc ttaaactacg aattgggcgc taaaacacct atgtcgtcta tatttgcctc
     3061 tttgatttta atggctatcg ttctttttat tacaccgctg gccgagcatt taccgattcc
     3121 gaccattgga ggaataattt taataattgc cttcaaactg attgatttta aaagcatttc
     3181 ccaaatttcc aaagcgagta aatcagaaat cgcggtactc actatcactt ttctttccac
     3241 tttgctattt gatttacaat ttgctatcct ggttggtgta tttttctccc tgagctttta
     3301 cttaatgaga acaacaactc ctgctgtaat tcccattgcc ccaaacccca ttaataccag
     3361 gagaaaattc atgaatgcaa aggttaataa ccttgacgaa tgtcctcaat tactaattgt
     3421 acgtattgat ggatcccttt tctttggtgc aatagagcac ataaaaaata aattaattgc
     3481 tttgcgcagc aattcacgca aaagaattct aattgtttgc agcggaatta attttgttga
     3541 tgttgccggg gctgaattcc tggcccggga ggcagagtca tggcgaagtg aaggaggtga
     3601 gttttatctc tgcagcctaa agaaaaaagt gcgggacttt ttagccaagg gatattcgga
     3661 aaggattggt atggaaaata tctatactaa taaggaaggc gcaatctcat ctatctatga
     3721 aaagctggat agacccactt gcgaagcctg cccggtgaag atttttttgg agtgcccgca
     3781 atgattcatg gtttatcaag aattaaaaga cggcttcaac attagcaatt gcttttttgg
     3841 tgaggcagca ccttgacatc acagataccg aaccaggttc ggcatgtcgg gataggtgac
     3901 agccattcca attgctttaa tcacatttcc gaaaaagata agatgagcga gaaagtggac
     3961 cagtggttta accaggtttg aattcccgtg ttaatctctt ccggaaaata atattatgat
     4021 caaagtcagc caggtaccta tctatcatca gcaaaattca tttgtcgaaa tcttactttt
     4081 attcaaaaag agagatgaaa acagttggca tctttttact atttatcgga attatgctgg
     4141 gatgtgcccc ggtaaaaacc aaaactcatt ttgatgataa gtttgatttc tccagttaca
     4201 aaaccttttg ctggctaagc ggatgtgagt tcgtttatga aggaccacaa cggtataatg
     4261 atagtctggc gattgataaa ataaggcacg cagtgataca agaattactg tcgaaaggtt
     4321 tgactcagga tgaaaacaat ccggatattc ttattgactt ccacattatt attgaagaaa
     4381 aaaccaacat aattactcat cgcgcacaat atgaagatga aagaggctat gcctggtatc
     4441 ccccatccta cgaggtgtac aactacctga aaggatctct tatcattgat atgattgata
     4501 acagtcaatc ccgaatggta tggcgatccc attctgtggg ctacctggaa gcctttccgg
     4561 ttataacgga tcaacaaatt gaacgatccg taagagaggc cttaaaagat tttcctcctg
     4621 tttttaataa ttaactccca gtttcccaga taagaaaggc cagtgccttc ctgctcaata
     4681 atctgaagct aatctgaagg tggatgcatt aggtaaaacc cttatttata tacattagga
     4741 ttttcccggt tccggttgga tcagataacc acgaattttg attaaacaaa atttgaaaat
     4801 catgaatatt gcacgaatga taaaagactt gaaagagcat tatgataacc ctgaccgcaa
     4861 ggtagtttta gatattgttg ccagttccag gtctgagtgg cagggttccg tggttccact
     4921 taatgagaag attaacaatc taagcaaact tatccatcat ttggagcgga actacaccta
     4981 tgttccttct aaattgtatg actccctggt aaggatgaat cagaattaca accggattgt
     5041 tcacaggaat ttcaagcagg aatattctgc aaatgtggcc tgaaa
//
LOCUS       DASWHX010000060         6859 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_1522029, whole genome shotgun sequence.
ACCESSION   DASWHX010000060 DASWHX010000000
VERSION     DASWHX010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 6859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6859
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1522029"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            576..1994
                     /locus_tag="VGA21_04310"
     CDS             576..1994
                     /locus_tag="VGA21_04310"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX9649758.1"
                     /translation="MKLQTCFLLFFTFISCSNPPEAESFLNPEIDALFSEWNSQGSPG
                     CALAVIQNGEIIYKKGYGMADLEHNIPIQPSTVFYCGSVSKQFVTLSILLLEEQGKLN
                     LDDDIRKYFTDFPDYGDTITIRNLIHHTSGIRDYLTLWDLTGRDYLDHISKHEVYELI
                     KRQKELNFVPGDRYLYSNSCYFMLAELVKMISGETLKDFAAKNIFEPLEMTNSHFHDN
                     YRHLIPNRAFSYAADGASYSNLIMRFDQVGSGGLYSSVEDLFLWDQNFYHNKLGKGSQ
                     SLIDKMHEEGTLNSGASAGYAFALVNGNYRGLRTVSHTGSLAGYRAVLTRFPDEHFSV
                     IILSNLASFNPARKAEEVAEIYLEDKMINESDSGSEVEGESGSEVEENTPEITDLLIF
                     QGRYFSEELEVYYTLSSEENKLFFAIGNLEPRPLVPAGKDRFSGEDGYPDFRFQKNSQ
                     GLVKGFVLDAGRVTNLKFTKVD"
     gene            complement(2079..2411)
                     /locus_tag="VGA21_04315"
     CDS             complement(2079..2411)
                     /locus_tag="VGA21_04315"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEX9649759.1"
                     /translation="MSKKNFTYIWEYLVKDNFLSEFITAYGPEGDWVQLFRKAGGYIK
                     TNLHRDTTNPNRFITVDFWKTRNDRDNFRKRFSDEFEKLDQRCEQFTESEKFLGDFDS
                     ISDHDPVD"
     gene            complement(2423..3739)
                     /locus_tag="VGA21_04320"
     CDS             complement(2423..3739)
                     /locus_tag="VGA21_04320"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HEX9649760.1"
                     /translation="MKRIKELAIQFWTLLTAAGPEGQRFRKRMAIILGLIIIGLWVNM
                     VLRANRGHGSQYDDFTGFSQDLIFDRINIYKEYAFESTSIGKYPPFFGLVYAPVAIFP
                     LVIGASIWFWFSIVLVVYAGKSVAMGVSRLSGETGQVNPLLWSIPLALGSIQIITNLE
                     TAQVNILIFSLVAFAIARFADKKDVQAGLLLGMATAIKLTPGLFVVYFAYKGAWRVVL
                     WAAIGGAICWFMVLPVVMGFTYYYDVMQSWVMMVGSFVSDGASAEGIAGFRHTNQSLS
                     AAFHRFFTETPAGGGIENFYINIVRIPHETAGIIVKSLNVAILIVLAWICRTPLGDRK
                     DPRLPLELSLVMIATLFLSPISWINHYVVMILAFGAAVYYIRAREYQDLNRKMMLQAL
                     IIAATLIMVSPRISQAFSLPFFGAVILAVALAKGILRGKKEMIAKE"
     gene            3801..4400
                     /locus_tag="VGA21_04325"
     CDS             3801..4400
                     /locus_tag="VGA21_04325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649761.1"
                     /translation="MIKRKLTKAILLIIVFSACSLDKPKYSENVVKDFNLGWWSDTRH
                     TALFLNNDHTEYGGREIVPETVAEIGYNQDFIIVKQHPNMFKEIEDRVLSNETETGYE
                     IKNAADTVYLPEEFVFSENGSLYLKKQGRDELDYLFPYRDVTYYYIIDIRNYRLRFWA
                     MDGNVFRFDNEIDFQMKRLQLAIPDEVTFTHVDISDESE"
     gene            complement(4407..5210)
                     /locus_tag="VGA21_04330"
     CDS             complement(4407..5210)
                     /locus_tag="VGA21_04330"
                     /inference="COORDINATES: protein motif:HMM:NF024962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649762.1"
                     /translation="MKPLGILLSKASVLLFVFIFFACDSSEDPGINCAVSGLSLMETS
                     TTNSMCGQPDGSLSVSGSGGMEPYMFSLNGVDFQALGNFTQLTSGNYTITIRDDNGCT
                     ASVSVIVQDETTLEATVSSTKTGCGQTSGSLDISAIGGTPGYMYKIGNSAFQDSESFT
                     NLAPGNYTVTVMDAEGCETSVSHRLLSTASWMQHILPIINTRCALSGCHDGNSGLPNW
                     TDLATVQQNAQRIKSRTGAREMPPAGAQQLTEDQISMIACWVDDGAMNN"
     gene            5385..>6859
                     /locus_tag="VGA21_04335"
     CDS             5385..>6859
                     /locus_tag="VGA21_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020597060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX9649763.1"
                     /translation="MRRVILITAIIAAPIWLNAQTQLSKKKIESLKSEAGEKVHSRAK
                     EAQVINDMIFSFAELGFQELRTSEYVTGILERNGFTIERGVSGMPSGWVAKWGSGKPV
                     IAIGTDIDCIPKASQKPGVAYHDPIIEGAPGHGEGHNSGQAVNVTAALVVKEIMEREN
                     LSGTIVVWPGVAEELVAGKSYFVRDGYFKDVDICLFTHVSNNLGVTWGGGAGTGLVSA
                     IFNFYGESAHSAGSPWRGRSALDAVELMNVGWNYKREHLNPLVRSHYVIVDGGDQPNV
                     VPSTASVWYYFREMTYPEIQKLYSMGIKMAEGAAMMTNTTMDYRILGNAWPRHFNKPI
                     AEATYENIKQVGLPTWSKEDNMLAKALQKEVGSDTTGLNIELSELQGPPRTPQSGGSD
                     DIGDISWVVPTITLRYPANIPRLQGHHWSSAVAMATPIAHKGVVAGAKVMAMTMIDIL
                     TKADLVKAAWDYFSEQTRDQKYEPFITAEDKPATYINANIM"
BASE COUNT         1992 a         1453 c         1566 g         1848 t
ORIGIN      
        1 cgaggagcaa attcctatgc gcgaggagca aattcctatg aacgaggagc aaaaaccctc
       61 gagcgaggag gtaaaaccct cgaccgaggg ggtcagaccc cctcgcgagg cgcctggctg
      121 gctggaaacg gcgtttttta attcatgctt attaccatgc tggcacgcga ttgcatcaat
      181 tgcatcgtca agccgatgaa ccttgcattt gtaatggttt cattctacaa aaccatcaag
      241 ttttcctgct tcccgtcctt ttagatgtgc cacacttttg ctgacattga aaatcaatca
      301 attttcaccc aaaataaaaa gtggaaggct acatgataat agcattttat tacaaatcat
      361 caaaggggcg atttatcttc ttgaaactat aatcagccac atgcgcataa atctccgtgg
      421 tactgccgct attattccct aaaattttcg aataacctta aatcggttcc gttttcaagt
      481 aaatgcgtcc cgtaagaatg ccgcaaccaa tgtaaagtca cctgaactga aattatttct
      541 taaattaccc ttcagaattt atcccgatta acaatatgaa actccagacc tgttttcttc
      601 tgttctttac ctttatctct tgttcaaatc cgcctgaagc cgaaagtttt ctaaaccctg
      661 aaattgatgc gctcttttcg gaatggaact cacaaggcag ccccggttgc gcgcttgctg
      721 taattcaaaa tggggaaatt atttacaaaa aagggtacgg catggccgac ctcgaacata
      781 atatccccat ccagccatca acggtttttt actgtggctc ggtttccaaa caatttgtta
      841 cactaagtat tttacttctg gaagaacaag gcaaactgaa tctcgatgat gatatccgca
      901 agtattttac cgacttcccc gactatgggg acactataac gattcgcaat ctcatccatc
      961 acaccagtgg catccgggat tatcttacgc tttgggacct gaccggaagg gattacctgg
     1021 atcatattag taagcatgaa gtgtatgagc tgatcaaaag acaaaaggaa ctgaactttg
     1081 tgccgggcga tcgctatctc tatagcaaca gttgttactt catgcttgcg gaactggtaa
     1141 aaatgatttc cggtgaaacg cttaaagact tcgctgccaa aaatatcttt gaacctcttg
     1201 aaatgaccaa ttcacatttt catgataatt accggcacct gatcccaaat cgcgccttta
     1261 gttatgccgc tgatggggcg agttacagca atctcattat gcgtttcgac caggtaggca
     1321 gcggtggcct ttattcaagt gttgaagatt tgtttttgtg ggatcagaat ttctatcaca
     1381 acaaactggg taagggatcg cagagtttga ttgataaaat gcacgaagag ggaacattga
     1441 attccggtgc aagcgcagga tatgctttcg cgctggtaaa tggcaattac cgcggacttc
     1501 ggacggtaag tcatacgggt tcgctggcag ggtaccgggc tgtactgact cgatttcctg
     1561 atgaacactt ttctgttatc atcctcagta acctggcttc atttaatccc gccagaaaag
     1621 cggaggaagt cgcggagatt tacctggaag ataagatgat taatgagagt gatagtggga
     1681 gtgaagttga gggtgagagt gggagtgagg tagaagagaa tactcccgaa ataactgact
     1741 tgctgatttt tcaaggcagg tatttcagtg aagaactgga agtgtattat acgctttcat
     1801 ccgaagaaaa taaactattt tttgctattg gaaatcttga accccggcca ttagttcccg
     1861 cagggaaaga tcgtttttcc ggcgaagatg gatacccgga tttcaggttt cagaagaatt
     1921 cacagggatt agtgaaaggt ttcgttttag acgcgggtag agttacgaat ttgaaattca
     1981 ccaaggtgga ttagaatagt gaaacagtga acagtgaaac agtgagcagt gaaacagtga
     2041 gcagtgagca attccctgtg aaggaataat agcatcagtc aatcaaccgg gtcatgatct
     2101 gaaatcgaat caaaatcacc caagaatttt tctgattcgg tgaattgttc gcagcgttga
     2161 tcgagctttt caaattcatc tgaaaatcgc tttctgaaat tatcgcggtc attcctggtt
     2221 ttccaaaagt caacagtgat aaacctgttg ggatttgtgg tatcccggtg aagattggtt
     2281 ttaatataac cgccggcttt tctgaataat tgtacccagt caccctcagg accataggcc
     2341 gtaatgaatt cactcagaaa attatctttc accaaatact cccaaatgta ggtgaagttt
     2401 tttttcgaca taattcagag gattattctt tggcaatcat ttcctttttt ccccgcaaaa
     2461 ttcctttagc aagagcaact gcaagaatta cggctccaaa gaaaggtaat gaaaaggcct
     2521 gggaaattcg tggagaaacc ataattaatg tagcggcgat aattaatgcc tgcagcatca
     2581 tcttgcgatt gagatcctgg tattccctgg ctcgaatgta atatacagcg gctccaaaag
     2641 ctagtatcat tactacataa tggttgatcc aggaaatggg agagaggaac aaggtggcga
     2701 tcatcaccag gctgagttcc aggggaagcc ggggatcttt tctgtcacct aatggtgtgc
     2761 ggcaaatcca tgccagcacg ataaggatgg cgacatttaa tgattttacg ataatgccgg
     2821 cggtttcatg aggtattctt acgatgttga tgtaaaaatt ctcaattccg cccccggcag
     2881 gagtctctgt aaagaatcgg tgaaaggcag cgcttaatga ctggttcgtg tggcgaaacc
     2941 cggcaattcc ttccgcgctt gcaccgtcgg aaacgaaact ccccaccatc attacccagg
     3001 attgcatcac atcgtagtaa tacgtaaacc ccatcacaac aggtaaaacc ataaaccagc
     3061 agattgcccc gccaatagca gcccagagaa ccacccgcca tgctccttta taagcgaagt
     3121 aaacaacaaa aagcccgggt gttaatttta tcgccgtagc catacccagc agcaacccgg
     3181 cctgtacatc tttcttatcg gcaaaacggg caattgcaaa agcgaccagg gagaaaataa
     3241 gaatattcac ctgggctgtt tccaggtttg tgattatctg aatggatccc agggccagcg
     3301 gaatcgacca caacaatgga tttacttgtc ctgtttctcc ggaaagtctg ctaacaccca
     3361 tcgccaccga cttaccggca tacacgacca gaacaatact aaaccaaaac caaatggagg
     3421 ctccaattac aagtggaaaa atagcaacag gcgcataaac caaaccaaag aagggtgggt
     3481 actttcctat agatgtgctt tcaaaagcat attccttata aatattaatg cggtcgaaga
     3541 tgagatcctg gctgaacccc gtaaagtcat cgtactgact gccatgaccg cggttggccc
     3601 gcagcaccat attcacccaa agaccaatga ttataagtcc gagaataatg gccatccgct
     3661 ttctgaacct ttgcccttcc ggtccggcgg cggtaagcag ggtccagaat tgaatagcta
     3721 attctttgat tcgtttcatc aacgccaatt tctaattgtt tttttacctt tggggcaaag
     3781 ttatttattc tgaagtaaaa atgatcaaaa ggaaattaac caaggcaatt ttattgatta
     3841 ttgttttttc agcgtgcagt ctggacaaac ccaaatattc tgaaaatgtg gtaaaagatt
     3901 ttaacctcgg gtggtggagt gatacccggc ataccgcact ttttctaaac aacgatcaca
     3961 cggaatatgg gggacgggaa attgtgcctg aaactgtcgc ggagattgga tacaatcagg
     4021 atttcataat tgtaaaacaa cacccgaata tgttcaagga aatagaagat cgggttttga
     4081 gtaatgagac tgaaaccgga tatgaaataa agaacgccgc tgatacggtt taccttcctg
     4141 aagaatttgt gttttctgaa aacgggagtt tatatctcaa aaagcaagga agggatgaat
     4201 tagattattt atttccctac agggatgtta cctattatta tatcatcgat atccggaatt
     4261 acaggcttag gttctgggcc atggatggaa atgttttcag gtttgataat gaaattgatt
     4321 ttcagatgaa acgccttcag ctggcaattc ccgatgaagt aacgttcacc cacgttgaca
     4381 tttctgatga atcagagtag ttctactcag ttattcatgg cgccatcatc gacccagcag
     4441 gcaatcattg atatttgatc ttcagttaat tgttgcgcgc cggccggtgg catttccctt
     4501 gcaccggttc tgctttttat tctttgggcg ttttgctgaa cagtggcaag atcagtccag
     4561 ttaggcagtc cgctgtttcc atcgtggcac ccggaaaggg cgcagcgtgt attaataata
     4621 ggcagaatat gctgcatcca gcttgcggta gataacagcc tgtgggaaac ggatgtttcg
     4681 catccttcag catccattac ggttaccgta taattacctg gtgccagatt ggtaaagctt
     4741 tccgaatcct gaaaagccga gttaccaatt ttgtacatat aaccgggagt accaccgatc
     4801 gcggaaatat ctaaacttcc tgatgtttgc ccgcaacctg tctttgtgct tgaaacagtt
     4861 gcttccaacg tagtttcatc ctgaacgatt acagacacgg acgcggtgca cccgttatca
     4921 tcccggatag ttattgtata atttccggaa gtcagttggg taaaattacc caacgcttga
     4981 aaatcaactc catttaaact gaacatgtac ggttccattc cgcccgatcc tgacactgaa
     5041 agacttccat cgggctggcc acacattgaa tttgtggtgg acgtctccat aagcgataga
     5101 ccggataccg cgcaattaat cccgggatcc tccgaagaat cacaagcaaa gaaaataaaa
     5161 acaaatagca atacgctggc cttactaagc agaattccaa gtggtttcat aggttacaag
     5221 gtgtttggac taaaagttat cctttcatca aaattcatac taatcatcag tgtttaaaca
     5281 tatatttcta aaattaatcc ctattgggct aatttttttg aaaggatagg tgatttttta
     5341 acgcctggcc cttaatttaa tgtaaaatct aatctactaa acccatgaga agagttattc
     5401 taattaccgc gattattgcc gctccaatat ggttaaatgc ccaaacccag ctttcaaaga
     5461 agaagattga atccctgaaa agtgaggctg gtgaaaaagt tcatagccgc gctaaagagg
     5521 cgcaggtgat aaatgacatg atattcagct tcgctgaact gggatttcag gagctgcgga
     5581 cgtcggagta tgtaacagga attcttgaaa gaaatggctt cactattgag cgtggtgttt
     5641 ccggaatgcc cagcggctgg gtagcaaaat ggggaagtgg taagccggta atcgcgattg
     5701 gtacagacat tgactgcatc cccaaggctt cacaaaagcc gggggttgcc taccacgatc
     5761 ctattattga gggtgcccca ggtcatggtg aaggacataa ttcgggacag gcagtaaatg
     5821 ttacagcggc actggtggtt aaagaaatta tggaacggga aaatctttcc ggtactatag
     5881 ttgtttggcc gggagtggcg gaagaattag ttgcaggtaa atcctatttt gtacgcgatg
     5941 gatattttaa ggatgtggat atatgcctgt tcactcatgt aagtaataat cttggtgtta
     6001 cctggggggg aggcgcaggt accggactgg tgtcggctat ttttaatttt tatggagagt
     6061 ctgctcatag tgcaggatct ccctggaggg gtagaagtgc gcttgatgca gtggagttga
     6121 tgaatgttgg ctggaattat aaacgcgaac atctgaaccc attggttcgc agtcactacg
     6181 taattgtgga tggcggtgac cagcctaatg ttgttccttc cactgcgtcc gtatggtatt
     6241 atttccgtga aatgacctat ccggaaattc agaaattgta ttccatggga attaaaatgg
     6301 cggaaggtgc agccatgatg actaatacaa ctatggacta ccgcattctg ggaaatgcct
     6361 ggccccggca ttttaataaa ccaatagctg aagctactta tgaaaacata aagcaggttg
     6421 gattgccaac atggagtaaa gaagacaata tgctggcaaa agccctgcaa aaagaagtcg
     6481 gttctgatac caccggtttg aacattgagc tgtcagagtt acaaggaccg ccgcgcacgc
     6541 cgcagagcgg tggaagtgat gatataggtg atatttcctg ggtggtgccg acaattacct
     6601 tgcggtaccc ggccaatatt ccccgcttgc agggacacca ctggtcaagt gctgtggcga
     6661 tggcaacccc cattgctcac aaaggagtgg tggccggagc aaaagtgatg gcaatgacca
     6721 tgattgatat tcttacaaaa gctgacctgg taaaggcggc ctgggattat tttagcgagc
     6781 agaccaggga tcagaaatat gaacccttta tcactgccga agacaagccg gccacgtata
     6841 ttaatgctaa tattatgaa
//
LOCUS       DASWHX010000061        10383 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_3369099, whole genome shotgun sequence.
ACCESSION   DASWHX010000061 DASWHX010000000
VERSION     DASWHX010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 10383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10383
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3369099"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1197
                     /locus_tag="VGA21_04340"
     CDS             <1..1197
                     /locus_tag="VGA21_04340"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX9649764.1"
                     /translation="YPAILLSRFNPSQTLKAGGSSQLVGKSARKILITFQFGITIILL
                     SGTYIIYNQTLFSINKDLGFDKSEVMVISIDISQIITNNPKPFQDAVAKIPGVELTSV
                     GMDALGPGSTNNSGNLTPEGSINPVLTTIFNADMNFLRTYGIEVLVGRDFNPELASDS
                     SAIIVNEEFVRQAGWNDPLGKKVVWGRGAGQPVIGVTKDFNFQDLRNKVNPVLITINK
                     WGFWNLAVRISTDNVSETMNRINQAWLNFENELPLEYYFVDERFARYYQSDERLLNII
                     GLFSLLSVFVTCLGLYGLTSFVVEQRLKEIGIRKVLGASSVSVTWLINKQFSSMLLIG
                     LALGVPITIWLGNLWLNDFAYKIRIEWWIFLGSGALTFGIALFTVSTKAIKAAFMNPV
                     DTLRYE"
     gene            1425..3815
                     /locus_tag="VGA21_04345"
     CDS             1425..3815
                     /locus_tag="VGA21_04345"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9649765.1"
                     /translation="MLKNFIKIALRNLLRQKGYSFINIFGLTAGITVSCLLFLYVWDE
                     LSYDKFHSNSHRIYRIIEKLASPDGDRLIGQTSPALEEALVSIPEIENTTSLYQLGGH
                     VNFFIGEERFLERNWKIVTPTITSVFDLDFIEGNPETAFNQPGLIISEQSAIRLFGTK
                     NVMGKNLKESEFGDLTITGIIKDLPSNSHLKLDFLFPIANLPQNLIQRMKSWDRYGAF
                     TYVLVNDGADIPAVEQKIEEISNPHWDANPARGGIWMQPIEDIYFGSDKIEFGSEITR
                     GQKFYVNLIAIVGIFIILLACINYTNLATARASQRSREIGMRKVNGALRWQLMLQFLG
                     ESVIIALFAFFLSLGAVDLLMPSFNQLTGKYFELRFALSQSVWLILLAISGFVGLAAG
                     AYPALYLSKLSPVKSLKGELRSGGKNLWFREGLVIFQFFISIIMLVSTIVVSQQVNYI
                     RNKELGFEKDNMLVIDINSGGVRRSFEAMKNEFSKIPGVTHVASSSRVPGEWKNISQV
                     NVNSALPGIDSIRSYFMCFDAEIISTYGMELAEGRNFSNNVQADSLTLIINETAARQL
                     GLEDPINKEIYVSFGQIVRMKVIGVLKDFNFQSLHDKVEPLIIGFWSNPVTSIDYFSL
                     RFNKDEIQNVIKAAAVVHGKFDQTTSMEYHFLDQQLAQFYLEETRQAKVFSIGSFLAI
                     IIASMGLLGLASFMMERRLKEIGIRKILGATKSSLFFRFNRIFMIQVVIAFVLSLPVS
                     FLLMDKWLSFFAFKIELTVLTFLLPGLAALVVAFLVAGFHITKASLTNPINILKTE"
     gene            3816..6167
                     /locus_tag="VGA21_04350"
     CDS             3816..6167
                     /locus_tag="VGA21_04350"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9649766.1"
                     /translation="MLRNFFFITIRNFWKQKFYSSINIFGFSVGLTTVILILLYVTHE
                     LSFDRFHVKSARLARVVENQYYSGQPVFPVAVTPIPLGPSLVEDYAEINQFARLMNTD
                     LLFEKEGEKYPESGIYVDPAFLNMFSFPLLYGEKETALASLENIIIDEELTKKYFGDE
                     NPVGKTIIVDGEEKVVSAVMKNFPKNTHIQANFIRSFESSALSQAQMQNWRSNTLFTF
                     IEVNQGVEVEELSQKISGQIKKFNTNSITEIYLQPITDIHLGEVQFVAEMGNKGNAVY
                     VNMFILIAVIILLIACINFMNLSTAKSVNRAKEVGLRKTLGAHRSHLIYQFMGESVFM
                     TLLAMIIAIFLADLALPFFNTLANTDLSINSISSATGTPLLVWGISLTLLTGIIAGSY
                     PAFFLSRFNPAYVLKGSFKGKREGSILRKVLVTIQFGISILLVIGTLVVLDQLNFMKT
                     MRLGINKENVIYVPQISNNNDVFKDQIMSLTSVKGIGFSNQHPAYVENSSSGFVWPGK
                     DPDQVALFHLQSVDGGYIEAMGLELAEGRNFNKHLPGDTASIIINEVAAKIMNMENPV
                     GQKLTEPFDITIIGVVKDFHFKSAHSRIEPLIMFTGRNNYNRTFIKIDGNNVSESVSQ
                     VKAVWNKLNQEQAMPYRFLDEDYEKLYQSEERTSKIFTNFTLLAFLISGLGLFGLSSF
                     TAEQRLKEISIRKVMGATSLKLFWLVSTDLTRLSIISILIAVPVGWYWMNSWLEGFAY
                     RIEISPVIFIVASLVGIIITFLTVSFQSLKVSASNPVNHLRNE"
     gene            6221..7168
                     /locus_tag="VGA21_04355"
     CDS             6221..7168
                     /locus_tag="VGA21_04355"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HEX9649767.1"
                     /translation="MKSVYIGILAVLVSFSAFSQKTKSDTFSDVTTIRLSASSGSAKF
                     IKSSGKEINVTLEYTYSDDEFEYKMEKNGSTLNLEEKFHRNYTRGNSHWSLEIPDGMD
                     VRFNSGSGDLEIDGLEIDIKSNQGSGSILASDTKGEFRLTTGSGDHEVRNHTGDLDLT
                     TGSGDIIISASSGELNFTTGSGDIEVNNMKGNLSATVGSGNIDARGIELLDDGIFTSG
                     SGDVDVRLNAATGASLSIVSGSGDATLDFGGQTINATITMEANERNGRIVAPFKFDTE
                     EVIERSGFSNNTIRKTVKLGSGNADVRISSGSGTAEVRK"
     gene            7605..8861
                     /gene="hutI"
                     /locus_tag="VGA21_04360"
     CDS             7605..8861
                     /gene="hutI"
                     /locus_tag="VGA21_04360"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014796167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HEX9649768.1"
                     /translation="MQTLIINIEGLVQVRDSTVIKVSGREMQELPVIQNAFLLLNKTI
                     IEAFGEMKDCPEKNNKQVIDASNRFVLPSFIDSHTHIVFAKSREEEFVDRIKGLSYQE
                     IAAKGGGILNSAKRLQKTSEEDLFRMTSERVKEIMMLGTGALEIKSGYGLTTQDEIKM
                     LRVARRIEAEFPILVKKTFLGAHAIPREYANRRDYIEEVINEMIPEIVDQKLAQYIDV
                     FCEEGFFTQDESVEILLAGKRHGLTPKVHANQLNRSGGVQAGVKTGAISVDHLECVGD
                     EEIKLLQGTATMPTLLPGAAYFLNTDFPPARKMIDNGLPVSLASDYNPGSSPTGSMPQ
                     MISMACINMKMTPEEAINAATINAAYAMQVSDLAGSITTGKLASVIITKKIPSISFIP
                     YAFGSNWIDKVFHKGKETNYSISSFS"
     gene            8979..9851
                     /gene="sucD"
                     /locus_tag="VGA21_04365"
     CDS             8979..9851
                     /gene="sucD"
                     /locus_tag="VGA21_04365"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020602619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HEX9649769.1"
                     /translation="MSVLVNKNSKVVVQGFTGSEGTFHSEQMIEYGTNIVGGITPGKG
                     GQVHLGKPVFNTVEDAVRGVGADVSVIFVPPAFAADAIMEAADAGIKVIVCITEGIPT
                     RDMIIAKEYLKGKDVTLVGPNCPGVITPGEAKVGIMPGFIHQKGSIGIVSRSGTLTYE
                     AVDQITKAGLGQTTCIGIGGDPIIGTTTKDAVMMLMNDPETKGIVMIGEIGGSMESDA
                     ANWIKENGTKPVVGFIAGQTAPPGRRMGHAGAIIGGAADTAAAKMEIMKKCGIHVVES
                     PADIGDTMLKALGK"
     gene            complement(9856..10227)
                     /locus_tag="VGA21_04370"
     CDS             complement(9856..10227)
                     /locus_tag="VGA21_04370"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX9649770.1"
                     /translation="MHNAIGWFEIPAKKLARAKKFYEAIFEVQMQEMNLGDGLKMALF
                     PVEPGTVGGAVCEHKDFYHPGEQGPLVYLNANPDIDKVLERIEKNGGKIFKQKTHISE
                     EYGYMAVFFDPEGNRIALHSK"
BASE COUNT         3177 a         1881 c         2238 g         3087 t
ORIGIN      
        1 tatccggcaa ttttactgtc ccgattcaac ccttcacaga cactaaaggc gggcgggagt
       61 tcgcaattag tgggaaagtc tgccaggaaa atattaatta catttcaatt tggaattact
      121 ataattctac tttccggaac ctacataata tataatcaga ccttatttag cataaacaaa
      181 gacttagggt ttgataagag tgaggttatg gttatttcaa tagatatttc tcagatcata
      241 actaataatc cgaaaccttt tcaggatgct gtagcaaaga ttccaggggt agaattaaca
      301 tcagtaggta tggatgccct gggtccgggc tcaactaaca actccggaaa tttgactccg
      361 gaaggctcaa tcaacccggt tctaacaaca atctttaatg ctgacatgaa ttttctgcga
      421 acctatggca tagaggtatt ggtaggaaga gattttaatc ctgaattagc gtctgatagc
      481 agtgcaatta ttgtaaacga ggaatttgtg cgccaggcag gctggaatga tccattaggc
      541 aaaaaggttg tctggggcag gggtgccggt cagcctgtta taggtgttac caaagatttt
      601 aattttcagg atttgaggaa taaagttaat cctgtactta tcactattaa taaatgggga
      661 ttctggaatc ttgctgttcg tatttctacg gataatgtct ctgaaacgat gaaccggatt
      721 aaccaggcct ggttgaattt tgaaaatgaa ttacctcttg aatattattt tgtggatgaa
      781 agatttgcca gatattacca gtctgatgaa agattattaa acataatagg attattttca
      841 ctcttaagcg tatttgtaac ctgccttggt ctttacggcc taacctcgtt tgttgtagaa
      901 cagcggctta aagaaatagg aatcagaaaa gttcttggtg catcatccgt tagcgtaacc
      961 tggttaataa ataagcaatt cagtagcatg ctcttaatag ggttggctct aggagttcca
     1021 attacgatat ggttgggaaa tttgtggctg aatgatttcg cttataaaat ccggattgaa
     1081 tggtggattt tcctgggatc cggtgcctta acttttggaa tcgctttatt tacagtttca
     1141 accaaagcaa taaaagcggc attcatgaat cctgttgata cattacgata tgaataaatc
     1201 attgttggaa ggaaaaaaac cccctgtagt cccccttaaa acaaggggga caattttccg
     1261 ggtactttcc cctccttatt ttaaggaggg ggcaggggtg gttcggaaaa ataagatgaa
     1321 caattttctg gcaggggtaa tttcccctcc ttattttaag gaaggcttgc cgaccgaagg
     1381 ttgggttagg ggaggtttga tttgaataat taaatataaa taagatgtta aaaaatttta
     1441 taaagatagc cttgaggaac cttttacgac agaagggata ctcttttata aacattttcg
     1501 gactcaccgc cggcattacg gtaagctgtc tgctcttttt gtacgtgtgg gatgaattga
     1561 gttatgataa atttcattcc aattctcata gaatctatag aataatagaa aagttggcct
     1621 cccctgatgg agatcgtctg ataggccaga catcacccgc gctggaagag gcacttgtct
     1681 ccattcccga aattgaaaat accacgagtc tttaccaatt agggggccac gttaattttt
     1741 ttattggtga agaaagattc ctggagcgaa attggaaaat tgttaccccg actattacct
     1801 ctgtttttga tcttgatttt attgaaggaa atcctgagac agcatttaat cagcccggac
     1861 ttataatttc agaacaatct gcaataaggc tgttcggaac aaaaaatgtg atgggaaaaa
     1921 atcttaaaga atcagaattc ggagatttaa ctatcacagg gattattaaa gacttaccct
     1981 caaattccca tctcaaactt gattttctgt ttccgattgc aaaccttcct caaaatttaa
     2041 ttcagcgaat gaaaagctgg gatcgatatg gagcttttac atatgtcttg gtaaatgatg
     2101 gagcagacat ccccgctgtt gagcaaaaga ttgaggaaat atccaatcct cattgggacg
     2161 ctaatccggc aagaggtgga atctggatgc agccaattga agatatttat tttggctcag
     2221 ataaaattga attcggttcc gagataacaa gaggacaaaa attctatgta aacctgattg
     2281 caattgttgg catcttcatt atcctccttg catgtataaa ttatacaaat ctggctacgg
     2341 caagagcttc tcaacggtca cgtgaaatcg gaatgagaaa agtaaacggg gcattaagat
     2401 ggcaacttat gttgcaattt ttaggagaat ctgtcatcat cgcacttttt gcatttttcc
     2461 tgtcacttgg tgcggtcgat ttgttaatgc cttctttcaa tcaattaaca ggtaaatact
     2521 ttgagctccg ctttgcacta agccaatcag tctggcttat cctattagct atttcaggat
     2581 tcgttggcct tgccgcgggc gcttatccag ctttatattt aagcaaactt tctccggtaa
     2641 aatcattaaa gggagaatta agaagtggag gtaaaaattt atggttccgg gaaggtctgg
     2701 ttatttttca gttttttatt tctatcataa tgcttgtatc gacaattgtg gtttcgcaac
     2761 aagtgaacta tatccggaac aaagaactcg gttttgaaaa agacaatatg ctggttattg
     2821 atataaacag cgggggcgta cgtcgaagtt ttgaagcaat gaaaaatgaa ttttcaaaga
     2881 tacccggagt aactcatgtg gcaagttcat ccagggttcc cggggaatgg aagaacataa
     2941 gccaggtaaa cgttaattca gcattgccgg gaattgattc catcaggagc tactttatgt
     3001 gttttgatgc ggaaataatt tcaacttacg gaatggagtt ggcggaagga agaaatttta
     3061 gcaacaatgt gcaggcagat tccttaactc taataattaa tgaaactgca gccaggcaac
     3121 tcgggctgga agacccaatc aataaagaaa tctatgtcag ttttggacaa atcgtaagga
     3181 tgaaagtaat cggtgttttg aaagatttca acttccagtc ccttcatgat aaagtggaac
     3241 cgttaataat tgggttctgg tcaaacccgg ttaccagcat cgattatttc agtttaagat
     3301 ttaacaaaga tgaaatacaa aatgtgatta aggcggcagc ggttgtacat ggcaaatttg
     3361 accagacgac ctcaatggaa tatcattttc tcgatcagca actggcgcaa ttttacctgg
     3421 aagaaacccg gcaggctaaa gtgttttcga taggctcatt cctggctatt ataattgcaa
     3481 gcatgggttt gctggggctt gcttctttca tgatggaacg acggctgaaa gaaattggca
     3541 ttaggaaaat tttgggcgct accaaatcct ctctcttttt ccggttcaat agaattttta
     3601 tgattcaggt tgtgatagca tttgtcctat ccctgccggt atcattcttg ctgatggaca
     3661 aatggctaag cttttttgct tttaagattg aactaactgt ccttacattt ctgctgcccg
     3721 gattagcggc tttggtggtt gcatttctgg tagcaggctt tcatattacg aaagcttctc
     3781 taacaaatcc gataaacatt ttaaagacag aataaatgct gagaaatttt tttttcatca
     3841 ccattagaaa cttttggaaa caaaaatttt actcatcaat caatatattc ggatttagtg
     3901 tcgggttaac tacagtaatc ctgattcttc tgtatgtaac ccacgaactg agttttgaca
     3961 gattccacgt aaagtcagca cgacttgcaa gggttgtgga gaaccaatat tattccggac
     4021 aaccggtttt tccagttgca gttactccaa ttcctttagg tccttctttg gttgaagatt
     4081 atgctgagat aaatcagttt gcgcggctga tgaataccga tcttttgttc gagaaggaag
     4141 gggaaaaata cccggaatcc ggaatttacg ttgacccggc ttttcttaat atgtttagct
     4201 ttccattact ttatggtgaa aaagaaacag cgcttgcttc tctggaaaat atcattattg
     4261 atgaagaact gacaaaaaaa tactttggtg atgaaaatcc ggtaggcaaa actattatcg
     4321 tggacggaga agaaaaagtt gtttctgccg tgatgaagaa ttttccaaag aatacgcata
     4381 ttcaggcaaa cttcattcga tcatttgaat cgagcgcact atcacaggca caaatgcaaa
     4441 attggaggtc taatacctta tttacattta ttgaggtgaa tcaaggagtt gaggttgaag
     4501 aattatctca aaaaattagc ggccaaatca aaaagtttaa tacaaattct atcactgaaa
     4561 tttatttgca gcccataacg gatattcatt tgggtgaggt tcagtttgtt gctgaaatgg
     4621 ggaataaggg aaatgctgta tatgttaata tgtttatcct gatagcagta ataatactac
     4681 tgattgcctg catcaacttc atgaatttat ctactgcaaa gtctgtaaac cgggcaaaag
     4741 aagtgggatt aagaaagact ttaggggctc acagatccca cctgatttat caatttatgg
     4801 gagaatctgt ttttatgacc ttattggcaa tgatcattgc catatttctt gccgatctgg
     4861 cactgccttt ttttaataca ttggcgaata ctgatctttc aataaactcc atttcaagcg
     4921 ctaccggaac accgctcctg gtttggggaa tctcattaac gctgttaacc ggaataattg
     4981 ccggaagtta tcccgccttt tttctatccc gatttaatcc cgcgtatgtg cttaaaggat
     5041 cattcaaagg gaagcgggag ggatcaatcc tcagaaaagt gttagtcact attcagtttg
     5101 gcatctccat attactggtc ataggaacac ttgttgtact tgatcaactc aatttcatga
     5161 agaccatgag gcttggaata aataaggaga atgtgattta tgttccgcaa ataagtaata
     5221 ataacgatgt gtttaaagat caaatcatgt ctttgacatc tgtgaaagga attggtttct
     5281 caaaccagca tcctgcttat gtcgaaaact catcatctgg ttttgtctgg ccaggcaaag
     5341 atcctgacca ggtcgcgcta ttccatttgc agtcggtaga tggtggttat attgaagcca
     5401 tgggacttga attagcagaa ggaaggaact ttaacaagca tctgccggga gatacagctt
     5461 ccataataat taatgaagta gctgcaaaga ttatgaacat ggaaaatccg gtgggtcaga
     5521 aattaacaga accctttgat ataacaataa ttggagtcgt taaagatttc catttcaaat
     5581 cagcacattc ccggattgaa ccacttatta tgtttaccgg caggaataat tataacagaa
     5641 catttattaa aatagatgga aataatgttt cggaatctgt cagccaggtt aaagccgttt
     5701 ggaataagct taatcaggag caagcgatgc cttataggtt tttggatgaa gattacgaaa
     5761 aactttatca atcagaagaa agaaccagta aaatctttac aaattttacc ttactggcat
     5821 tccttatttc aggcctcggc cttttcggtc tttcatcttt tacagctgaa caacgcttaa
     5881 aggaaattag tatccgtaaa gtgatgggcg cgacttcgct aaaattgttc tggctggtaa
     5941 gtacagacct cacccgatta agcataatat caattttgat tgcggtgccg gtggggtggt
     6001 attggatgaa ttcatggctg gaaggatttg cctatagaat tgagatttca ccggtaatat
     6061 ttattgtagc atcattggtt gggataatta ttactttcct aaccgtaagt ttccaatcct
     6121 taaaagtttc agccagcaat ccggtaaatc atttgagaaa tgaataatta catgcaacct
     6181 tttacaaacc ggatggtata tagagaaaca ataacacaat atgaaatcag tatatatagg
     6241 aattttagcg gtattagttt ctttttcagc tttttcacag aaaacaaaaa gtgacacttt
     6301 ttccgatgta acaaccatcc ggctatcagc aagttcaggt tcagccaaat tcataaaatc
     6361 ttcaggaaag gaaattaatg tgactctgga atacacctat tcggatgatg aatttgaata
     6421 taaaatggaa aaaaacggga gcacattgaa tcttgaagaa aaatttcaca gaaattatac
     6481 ccggggtaat tcccactgga gtcttgaaat tcctgatggg atggatgtac ggtttaattc
     6541 cggttcgggt gaccttgaaa ttgatggcct ggaaatagat atcaaatcta atcaaggatc
     6601 agggtcaatt cttgccagcg atacgaaagg agaattcagg cttactaccg gatccggaga
     6661 tcatgaagta agaaaccata caggagactt agacttgaca accggttcgg gagatattat
     6721 aatatctgcc tcatcgggag agttgaattt taccaccggc tcgggagata ttgaagtcaa
     6781 taatatgaag ggtaatttat ccgctaccgt tggcagtggc aatatagatg cccggggaat
     6841 tgaacttctt gatgacggaa tattcacctc cggttcaggt gatgttgatg tacgattgaa
     6901 tgcggctacc ggcgcttcat tatctattgt aagcgggagt ggtgacgcga ctcttgattt
     6961 tggagggcag acaataaatg ctaccataac aatggaggca aacgaaagaa atggaagaat
     7021 tgtcgcgccg tttaaatttg atacggagga ggtaatagaa agatcgggtt ttagtaataa
     7081 tacgatcaga aaaaccgtca agctcggatc aggtaatgcc gatgtgagaa tttcatccgg
     7141 gtcaggcacc gcggaagtta gaaaatagtc tatgtgtcaa ctaatatttc agtaggtcat
     7201 taagcgtttt tagagaaagt tgaagttctg aacttcctat tttccctatt ctctctttaa
     7261 gtctgttcct ggtacagaac gaataaggaa agattaacat ctcagattcc ttttttaaac
     7321 catttgtatt tgatggcatc gaaacagggt ttcccttgta tcttttttag ccgggtagta
     7381 agtggcccag gcaatttgct ctaggaaggg aaagtgacct ggccgtctcc gataattctt
     7441 caaggaattc gtccggaagg gaactggtaa aggcggccat attgattaaa ttttccacct
     7501 aaattgcaaa taccgtgatc cccatttagt ccatatatat attaataaag tttttttgat
     7561 caagataata gcattatcat tatctgtgaa tagtacgtac tttaatgcaa acattaatca
     7621 tcaacattga gggccttgtt caggtacgcg acagcactgt aataaaagtt tccgggaggg
     7681 aaatgcagga attacccgtc attcaaaacg ctttcctgtt attgaacaaa actattattg
     7741 aagcatttgg tgaaatgaag gattgtcctg agaagaacaa taaacaggtt attgatgcaa
     7801 gcaatagatt tgttttacca agctttattg attcccacac ccacatagta tttgccaaaa
     7861 gcagggaaga ggagtttgta gacagaatta aaggattgtc ttatcaggaa attgcggcaa
     7921 aaggcggagg cattctaaat tctgcaaaga ggttacagaa aacttcggaa gaagatctgt
     7981 tcaggatgac atctgagcgg gttaaagaaa ttatgatgct gggaaccggg gcgctggaaa
     8041 taaaaagtgg ctatggatta accactcagg atgaaattaa aatgttacgc gtggccaggc
     8101 ggattgaagc agaatttccc attcttgtta agaaaacatt tcttggtgct catgctattc
     8161 ctagagaata tgcaaatcgc agggattata ttgaggaggt aatcaatgaa atgattcccg
     8221 aaatagtaga tcagaaactt gcccagtaca tcgatgtttt ttgtgaggaa ggttttttta
     8281 cacaagatga atcagtcgaa attttgctgg ccggtaagcg ccacgggtta acccctaagg
     8341 tccatgccaa ccagttaaat cgttcaggag gagtccaggc aggagtgaag acaggcgcta
     8401 taagcgttga ccatcttgag tgtgtaggtg atgaagaaat caaattattg cagggtaccg
     8461 ctacgatgcc aactctgctg cccggcgcgg cttactttct taatacagat tttcctccgg
     8521 ccagaaaaat gatagacaat ggcttgccgg tttctcttgc ttctgattac aatcccggaa
     8581 gcagtccgac aggcagcatg ccacagatga tctccatggc ctgcattaac atgaaaatga
     8641 ctcctgaaga agcaataaac gcggcaacaa ttaatgccgc ctacgctatg caggtttccg
     8701 atctggcagg atctattaca actggaaaac tggcctcggt tattattaca aaaaaaatcc
     8761 cttccataag ttttatccct tatgcctttg gaagtaactg gattgacaaa gtatttcaca
     8821 aagggaagga aacgaattac tcaatcagtt catttagtta aatgtcaaca agttccggag
     8881 aatgttgaca atcttacttt ttgttactca taatattgct aaacctctca aaaaccctat
     8941 ttttgactta aagaatcaaa cccttaatcc ctttaattat gagtgtatta gttaacaaga
     9001 attcaaaagt cgtggtgcag ggttttacag gttccgaagg aacatttcat tctgaacaaa
     9061 tgatagaata tggtacaaac atagttggcg gtattactcc gggcaaaggt ggacaggttc
     9121 atttgggaaa gcccgttttt aatacggttg aagatgctgt tcgtggggtc ggggctgatg
     9181 tctctgtgat ttttgtacca ccggcatttg ccgccgatgc tatcatggaa gcagctgacg
     9241 ctggaattaa agttattgtt tgtattacgg aaggtatccc aaccagggat atgattattg
     9301 ccaaagaata tctgaaagga aaagatgtaa ctctggttgg ccctaactgc ccgggtgtta
     9361 ttactcccgg tgaagctaaa gtaggaatca tgcctggatt cattcatcaa aaaggatcta
     9421 tcggaattgt ttcccgatcc ggaacactaa cctacgaggc cgttgatcaa ataactaaag
     9481 caggacttgg gcaaaccact tgcattggaa taggcggtga tccaataata ggaaccacca
     9541 ccaaagatgc ggttatgatg ctgatgaacg atcctgaaac taaaggaatt gttatgattg
     9601 gtgaaatagg tggaagcatg gaatcagatg ccgccaattg gattaaggaa aacggcacca
     9661 agccggtggt tggattcatt gcgggacaaa ctgctcctcc gggaagaaga atgggtcatg
     9721 cgggtgcaat aattggtgga gcggcggata cagcggctgc aaagatggag atcatgaaaa
     9781 agtgtggcat tcatgttgtg gaatcaccgg ctgatatagg ggatacgatg cttaaagctc
     9841 tcggtaaata attttttatt ttgaatgcag ggcaattctg ttgccttcag gatcaaaaaa
     9901 taccgccata tatccatatt cctcagagat gtgtgttttc tgcttaaaaa tttttccgcc
     9961 attcttttca attctttcca gtaccttatc aatgtccgga tttgcattaa gataaaccag
    10021 cgggccttgc tctccaggat ggtaaaaatc cttatgctca caaacggctc caccaacggt
    10081 tcccggttca acgggaaata gagccatctt gagaccatcg cctaagttca tttcctgcat
    10141 ctggacttcg aagatagcct cataaaattt ttttgctctt gccagcttct ttgccggtat
    10201 ttcaaaccat ccaatcgcgt tatgcataat ttatgatgtt ttaatacttc cattttacca
    10261 aaaattcagg attttatcaa cctaatagta aaaatacttc tacaataaca ttagaaattg
    10321 tcccctttgt tttaaggggg accatagggg gtttaaatac tatttttaga accgcccctg
    10381 ccc
//
LOCUS       DASWHX010000062         9422 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_414068, whole genome shotgun sequence.
ACCESSION   DASWHX010000062 DASWHX010000000
VERSION     DASWHX010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 9422)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9422)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9422
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_414068"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(134..1354)
                     /gene="mraY"
                     /locus_tag="VGA21_04375"
     CDS             complement(134..1354)
                     /gene="mraY"
                     /locus_tag="VGA21_04375"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010611245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEX9649771.1"
                     /translation="MLYYLFDYLDKNFDLLGAGVFKYISFRSGGAVIVSLLITVFFGK
                     QLIEILRKKQVSESVRDLGLEGEINKRGTPTMGGIIIIAAILIPTLLFAKLDNVYVLL
                     LLISTLWMGAIGFLDDYIKIFKKNKEGLAGRFKIIGQIGLGLIVGLTLYFNDNVVVRE
                     FKEKQVVVQSQNVEETLPEFVDVKSTKTTIPFLKDNELDYEILVSFLGKDFTWIFYVL
                     VVIFIVTAVSNGANITDGLDGLAAGTTAIIAVTLAALAYVSGRFDFSSYLNIMYIPNT
                     GELVIFCAALVGACIGFLWYNAFPAQVFMGDTGSLSLGGIIAVLALTIRKELLIPLLC
                     GIFLIENLSVVIQVFWFKYTKKKYGEGRRVFLMSPLHHHYQKKNIPEPKIVTRFWIVG
                     ILLAIMSLATLKLR"
     gene            complement(1354..2817)
                     /locus_tag="VGA21_04380"
     CDS             complement(1354..2817)
                     /locus_tag="VGA21_04380"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017731844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEX9649772.1"
                     /translation="MPVLKDILKKVEITSITGKLETEILEVNFNSQSIRKGDLFVAIK
                     GIQVDGHKFIEQAIKNGAVAIVCESYPKKLNEEITYVSVKDSPLALSIISSNYYGNPS
                     GKLIVVAVTGTNGKTTIVSILFQLFRKLGYDVGLISTVGNRINDEIIPTTHTTPDALI
                     INALLDQMVQKGCTHCFMEVSSHAIVQQRVAGLRFTGAIFSNVSHDHLDYHKTFEDYI
                     KAKKKLFDKLPSSAFALVNVDDKRGPVLLQNTKALKGTYSLKNKSEYKAKIKSNSLRG
                     LELEIGGKDVWFKLMGDFNAYNLLAAYSAAILLGEENDEVLKHLSDVNPAPGRFEQVA
                     NTSNITAIVDYAHTPDALQNVLTTINSFRSGKEKLITVIGCGGNRDKAKRPIMAAIAC
                     DLSTTVIFTSDNPRDEDHNEIIKDMREGITGARKKKTIVLPKREDAIKTACLLAEEGD
                     IILVAGKGHEKYQEIKGVKYPFDDIEVVHRILNMFKK"
     gene            complement(2817..4910)
                     /locus_tag="VGA21_04385"
     CDS             complement(2817..4910)
                     /locus_tag="VGA21_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013455404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEX9649773.1"
                     /translation="MNIKGSILIRARIAFLVMFMFALAVVYRIIILQFVEGEQWSEIS
                     RKVSLQYKTVSATRGNIFSDNGSLLATSVPYYRVAMDPTRPSNEVFQKEIDSLALKLA
                     RTFREKSPQEYKREITNARVTGRKYIFLTRDEIDYQEKKKMEEWPVFRYGRLDGGVIF
                     EKVDKRFMPFSYLAYRTIGYINENNEGAGLEYSFNNYLAGKDGEALFQKISGGSWKPV
                     PDAHSLRPSEGYDIETTIDVNLQDVAQAALLKSLSAHHADYGALIVMEVKTGEIKAMS
                     NLSRTANGKYAELYNYAMGRHGLREPGSTFKLATMIALLEETDIKLEDTIQTGKGTYK
                     IYNETVRDHEEGGFGTISIQKSFEVSSNIAMAKLAEKYFTSKPSKFFEYLKQLHLTEP
                     LGFQMEGEGKPVVKEPKDWSGITLPWMAHGYGLELTPIHTLTLYNAVANNGTMIKPII
                     VKSIQKADKVKEKFESEILVKKICSDKTLNKLRILLEGVVNSGTASNIKNSHYQIAGK
                     TGTAQLLRNGRHSRNYMTSFVGYFPAEAPLYSAIVVIENPKGFWQYGSNVAAPVFKEV
                     ADKIFASRIEMHDPIPKEYRAELGVFPVIQAGYKADLELICNELGISNHAKTNDEWVR
                     AARNSNSVDWVSDKVTKALVPNVTGMTLRDALFLLENKGMQVEFKGKGRVTKQSEIPG
                     KKIVKGDKILLTLGS"
     gene            complement(4907..5269)
                     /locus_tag="VGA21_04390"
     CDS             complement(4907..5269)
                     /locus_tag="VGA21_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009194705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsL-like putative cell division protein"
                     /protein_id="HEX9649774.1"
                     /translation="MENKFKINKQKGESRSLFRRMENLLKANDLFEDGIPVNYLPRIL
                     FVAFLLIIYIWNGHQAERMVRKIDRMQGEVEDLRADFTTLKADYMYARLQSEVAKRVA
                     KMGLVESENPPYKIELKK"
     gene            complement(5276..6193)
                     /gene="rsmH"
                     /locus_tag="VGA21_04395"
     CDS             complement(5276..6193)
                     /gene="rsmH"
                     /locus_tag="VGA21_04395"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009284663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEX9649775.1"
                     /translation="MGFAMSKYHTPVMLYETLEGLKVNPSGIFVDLTFGGGGHSAAIL
                     STLTSGKLFAFDQDPDARENAKAMQSKNFTFIDSNFRYFAQYLKMHKVTEVDGILADL
                     GVSSHQIDTPERGFSTRFESELDMRMNRDAEKSALTVINEYSEEKLINILKSYGEIQN
                     ARQIAKSIIAARIKNKISTVEELKTILNAFAPRGRESKFFAKIFQGIRIEVNDELVAL
                     KEMLIQSSGVLKKIGRLVVISYHSLEDKLVKNFMQSGNFRGELEKDIYGNIIRPFNPV
                     TRKPLVPSQTELIENKRSRSAKLRVAKKN"
     gene            complement(6226..6669)
                     /gene="mraZ"
                     /locus_tag="VGA21_04400"
     CDS             complement(6226..6669)
                     /gene="mraZ"
                     /locus_tag="VGA21_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017731840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEX9649776.1"
                     /translation="MPFFTSEYECKLDAKGRLVLPAKIKSSLPEATENEIVIRRGFEP
                     CLVLYPLVEYKKIFSKVAGLSEFNEEFRTLQRNFFRGNSMAELDGNGRFLIPKPMLRH
                     AQIEKHAIVVGMGNRVEIWNPKLYEEYLIKDQSKFSELAQKYLAD"
     gene            complement(7007..7384)
                     /locus_tag="VGA21_04405"
     CDS             complement(7007..7384)
                     /locus_tag="VGA21_04405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649777.1"
                     /translation="MDSNDYPVKGDNVIKVNTSKSDQENFDDFSRHLVSKGFSFESRD
                     SELLLLVTNSKEADFQIHHKFIVSFRSETIYVRSEIRLGYVEGSGWESWYYTDVWNSV
                     SKAFNYFFPFIYEYSKDIEFEKV"
     gene            complement(7464..7769)
                     /locus_tag="VGA21_04410"
     CDS             complement(7464..7769)
                     /locus_tag="VGA21_04410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649778.1"
                     /translation="MASTIFSEATSTEIIEKTGAEGFIETKNGIHIAGNITKEAIKKI
                     EKQTGKKVVTHKNYKELDNPEIRKELIQKTPDEEKDFDETLKKLLNVPPPKRDKKDD"
     gene            complement(7747..8400)
                     /locus_tag="VGA21_04415"
     CDS             complement(7747..8400)
                     /locus_tag="VGA21_04415"
                     /inference="COORDINATES: protein motif:HMM:NF014548.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
                     /protein_id="HEX9649779.1"
                     /translation="MSKKIKKGNPKIAIFEGEEIRRIAVDGEWYFSIEDVIRVLTDSV
                     NPKDYINKLRKRNESLREGWGQFVHTLPIKTKGGTQNVNCASTEGVFRIIQEVPSKKA
                     EPFKRWLARVGKERLDEIEQPAKAIERAKGYYLAKGYIPKWVQTRLTGIDTRHTFTDS
                     LKDSGIKESYEYAILTDELYKSSFGFSTTEYKKHKGLSKKDSLRDNMTPRIGFNDFQ"
     gene            complement(8548..>9422)
                     /locus_tag="VGA21_04420"
     CDS             complement(8548..>9422)
                     /locus_tag="VGA21_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002702051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEX9649780.1"
                     /translation="DRIMGYIVRRKGKQMRPMFVFLTAGMCGGITESTYRGASLIELL
                     HTATLVHDDVVDESNFRRGFFSINALWKNKIAVLVGDYLLSRGLLLSVDNNDHEILRI
                     VSNAVREMSEGELLQIEKARNLDITEEVYYDIIRQKTASLIASCCAVGSSSAGQNGEQ
                     VELMRKFGEKVGMAFQIKDDLFDYGENEIGKPVGIDLKEKKMTLPLIYSLLQSSWSDK
                     RRIINIVKNQSEDKKKLKEVIDFVNQKGGIDYAKSVMGKFHQEALSLIGDIRESEYKT
                     SLIHLLQFTIERTK"
BASE COUNT         2546 a         2102 c         1646 g         3128 t
ORIGIN      
        1 tccaattctt ttttaaattc ttctttaatt gatcctgaat cggagacaaa cacttcatat
       61 cccgtggcta tcgccaatat tgcggcccca acaccacttt ctccagcacc caatatggtt
      121 atcttctttg ccattatctt aatttcaatg ttgccaggct catgattgcc agaagtatcc
      181 ccacaatcca gaacctggtc actattttcg gttccggtat atttttcttt tgataatgat
      241 gatgcaatgg tgacatcagg aatactcttc gcccttcccc atatttcttc tttgtgtatt
      301 taaaccagaa aacctggatc accactgata gattttctat taaaaagatt ccacacaaca
      361 agggaatcag gagttctttg cgaatcgtga gggcgaggac tgcaatgatt ccgcccagac
      421 ttaaactgcc tgtatctccc atgaagacct gtgccgggaa tgcgttgtac cataagaatc
      481 caatgcaggc tcctaccagt gcagcacaga aaattaccaa ctcacctgta ttggggatgt
      541 acattatatt caggtaagaa ctgaaatcaa agcggcctga aacataggcg agtgctgcaa
      601 gggtaacggc aattattgcc gtagttcccg cagccagacc atcaagcccg tcggttatat
      661 tggcaccatt tgaaacggca gtcactataa agattaccac cagaacataa aaaatccagg
      721 taaagtcttt tcctaaaaag gaaacaagga tttcataatc aagttcattg tctttaagaa
      781 atgggatggt tgtcttggtg gattttacat ccacaaattc aggtagcgtt tcttcaacat
      841 tctgactttg aacaaccact tgtttttctt tgaactctct tacaaccaca ttatcattaa
      901 aatagagcgt caggccaaca ataagtccaa gaccaatttg cccgataatc ttaaatctgc
      961 cagccaggcc ttccttgttt ttcttaaata tttttatgta atcatcaagg aatccgattg
     1021 ctcccatcca taaggtggaa atcagtagta acaagacata aacattatcc agtttggcaa
     1081 ataacaaagt tggaatgagg atagcggcga taataataat gccgcccata gtgggagttc
     1141 cccttttatt aatttctcct tcaagaccaa ggtcgcgaac agattcacta acctgctttt
     1201 tccgaagaat ctcaataagc tgttttccga aaaatacagt tatcagtaat gaaacaatta
     1261 ccgctcctcc ggaccggaat gaaatgtatt tgaatacacc ggctccgagg agatcaaaat
     1321 tcttatccaa ataatcaaac aggtagtaga gcattacttc ttaaacatgt ttaaaattct
     1381 atgaaccact tctatatcat caaatgggta ttttacgccc tttatttcct ggtatttttc
     1441 atggcctttt ccggcaacca atatgatgtc accttcctcg gcaagcaggc atgcggtttt
     1501 aatcgcatct tcccgcttag gcagaacaat tgttttcttt ttcctggctc ctgtaattcc
     1561 ttcgcgcata tctttgataa tctcattgtg atcttcatct ctcggattat cagatgtaaa
     1621 aattacggtt gttgagagat cgcatgcaat tgccgccatg attggtcttt tggctttgtc
     1681 acgattgccg ccacatccta ttactgtaat taatttttct ttgccacttc tgaaagaatt
     1741 aattgtggta agaacatttt gcaatgcatc cggcgtatgg gcataatcga caatggcagt
     1801 gatattcgaa gtattggcca cctgctcaaa acgtcccggg gccggattca cgtctgataa
     1861 gtgtttcagc acctcatcat tttcttcacc caaaagaatt gcagcgctgt aagcggcaag
     1921 tagattatac gcattgaaat cccccatgag tttgaaccag acatctttgc cgcctatctc
     1981 aagttcaagc ccgcgcaggg agttgctctt aatctttgcc ttgtactcgc ttttgttttt
     2041 tagggaatag gtgcctttca gcgccttggt attctgcaaa agtaccggtc ctcgtttatc
     2101 atctacatta accagggcga atgctgaaga cggaagttta tcaaataact ttttctttgc
     2161 ctttatgtaa tcttcaaatg ttttgtggta gtcaaggtga tcatgtgaaa cgttcgaaaa
     2221 aattgcaccg gtgaacctga gtccggcaac acgttgctga acaattgcat gactgctcac
     2281 ttccatgaaa caatgagtac agcctttttg aaccatctga tcaagcaaag cattgatgat
     2341 taaggcatca ggcgtagtat gtgtggttgg gataatttca tcattgattc tatttcccac
     2401 agttgagatt aatccgacat cataacctaa ttttctgaac aattgaaaaa gtatggaaac
     2461 aatagtagtt ttaccattag tgccggttac cgccactacg atcaatttcc cgcttggatt
     2521 tccgtaataa tttgaggaaa ttattgataa agccagcggg ctatccttta cagaaacata
     2581 tgtaatttct tcatttaatt ttttggggta agattcacaa acgatggcta ccgcgccatt
     2641 cttaattgcc tgctcaataa acttatgacc atcaacctga attcctttta tggcaacaaa
     2701 cagatcaccc ttgcgaatgc tttgtgaatt gaagttcact tccagaattt ccgtttccaa
     2761 ctttccggtt atagaagtta tttccacttt tttcaatatg tctttcaata cgggcatcac
     2821 gatccgagtg ttaatagtat tttatctcct ttaacaattt ttttcccggg aatttccgat
     2881 tgttttgtta ctcttccctt acccttaaat tccacctgca ttcctttgtt ctcaagaaga
     2941 aacaaagcat ctctcaaagt cattccggtt acattgggca cgagagcttt cgttacttta
     3001 tcagagaccc aatccaccga gttggaattt ctggctgccc gcacccattc gtcattggtt
     3061 ttggcgtgat tggaaattcc caattcattg caaatcagtt caagatccgc tttgtagccg
     3121 gcctgaataa ccggaaacac gcccaattca gcccgatatt ctttcggaat tggatcatgc
     3181 atttcaatcc gcgaagcaaa aattttatca gcaacttctt taaataccgg tgcggccaca
     3241 ttgcttccat attgccagaa tccctttgga ttttctatta ctacgatggc actatataat
     3301 ggtgcttcgg ccggaaaata accaacgaag gaagtcatat agtttcggga atgccttccg
     3361 ttcctcagaa gctgagcagt tccggtttta ccggcaatct ggtaatgtga atttttaata
     3421 ttgctggctg ttcctgagtt cacaactcct tccagtaaaa ttctcaactt atttaatgtt
     3481 ttatccgagc atattttttt aacaaggatt tcagattcaa atttctcttt tactttatcc
     3541 gctttttgaa tagattttac aataatgggt tttatcatgg ttccattatt tgcaactgca
     3601 ttatataaag ttaaagtgtg gattggtgta agctctaatc catatccgtg agccatccag
     3661 ggaagagtaa ttccactcca atccttcggc tctttcacaa ccggcttgcc ttccccttcc
     3721 atctgaaagc cgagaggctc ggtaaggtgc aattgtttca gatattcaaa gaattttgaa
     3781 ggctttgaag taaaatattt ctccgcgagt ttagccatgg caatgttgga agaaacttca
     3841 aatgattttt gaatgcttat ggttccaaat ccaccttctt catgatcgcg gacagtttcg
     3901 ttataaattt tatacgttcc ttttcccgtt tgaattgtgt cttccagttt aatatcagtt
     3961 tcttccagta atgcaatcat tgtggccagc ttgaaagtgg acccaggttc gcgcaatcca
     4021 tgcctgccca ttgcataatt atagagttca gcatatttgc cattcgcagt tctgctcagg
     4081 ttcgacatcg ctttgatttc tcctgttttc acttccatca cgatcaacgc tccataatca
     4141 gcatggtgag cgctaagaga cttcagcagt gctgcctggg ctacgtcctg caggttaaca
     4201 tcaatggtag tttctatatc atacccttcg gatggtctca gggaatgagc atccggaacg
     4261 ggcttccaac tacctccgga aattttttga aacaatgctt cgccatcttt tccggccaga
     4321 taattattaa agctgtattc caatcccgca ccttcattat tttcatttat ataaccaatg
     4381 gttctgtatg ccaggtaaga aaatggcata aatctcttat ctactttttc aaagattacg
     4441 ccaccatcca gccggccgta tcggaaaact ggccattctt ccattttctt tttttcctgg
     4501 taatcgattt cgtcccgggt caggaaaata tactttcgtc ccgtaaccct ggcattggtt
     4561 atttcccgtt tgtattcttg tggagatttt tctctaaagg ttcgtgcaag ttttagggcc
     4621 agcgaatcaa tttctttctg gaacacttca ttactgggcc ttgtgggatc catcgccacc
     4681 cggtaataag gaactgaggt cgctaataag cttccgttgt cagagaaaat atttccacgc
     4741 gtagccgata ctgttttgta ttgtaagctc acttttctgg agatttctga ccactgttcg
     4801 ccttcaacaa attgcagtat gatgattctg tataccaccg cgagtgcaaa catgaacatc
     4861 accaggaaag caatgcgggc gcggattaat atggaacctt tgatattcat tttttaagct
     4921 caattttata tggaggattc tcactttcca ccaaacccat tttggccaca cgtttagcaa
     4981 cctctgattg cagtcgtgca tacatgtagt cggctttcag cgttgtgaag tcggccctca
     5041 aatcttccac ttcaccttgc atccgatcaa ttttccgaac cattctttct gcctgatgcc
     5101 cattccagat ataaatgatg agtaagaaag cgacaaaaag aattctgggc aggtagttta
     5161 ccggtattcc atcctcaaac aggtcattcg ctttaagaag attttccatt cttcggaaca
     5221 agctcctgga ttctcctttt tgtttattga ttttaaattt gttctccact tcttttcaat
     5281 tttttttcgc aactcttaac tttgcgctgc gagatctttt gttttcaatt aattccgttt
     5341 gtgatggaac caagggcttt cgcgttaccg gattaaatgg ccggattatg ttaccatata
     5401 tatctttctc caactcacct ctaaagtttc cgctttgcat aaagtttttt accaatttgt
     5461 cttccaggga atggtaggaa attactacca gacgaccgat ttttttcaac acgccggatg
     5521 attgaataag catttccttc agagcgacca gttcatcatt tacttcgatt cggattccct
     5581 gaaagatttt tgcaaaaaat ttagattccc tgccgcgggg tgcaaatgca ttcaaaatag
     5641 tttttaattc ttcaacagtg cttatcttgt tttttattct cgcggcgata atggatttag
     5701 caatctgtcg cgcattttgt atttctccat aagatttgag aatgttgatt agtttttcct
     5761 ctgaatattc attgattact gttaacgcgg atttctccgc gtcccggttc atacgcatat
     5821 ccaattcgga ttcaaagcgg gttgagaatc ctctttccgg tgtatctatc tggtgtgaag
     5881 aaactccaag gtcagccagt attccgtcaa cctcagttac tttatgcatt ttcaggtatt
     5941 gcgcgaaata cctgaagttg gaatctataa aggtaaaatt ttttgattgc atcgcctttg
     6001 cattttcgcg ggcatcggga tcctgatcaa aggcgaataa ttttccggaa gtaagcgtgg
     6061 agagaatggc ggcagaatgt ccacccccgc cgaaggtaag gtcgacgaaa attcctgagg
     6121 gatttacttt cagcccctca agggtttcat acaacattac aggggtgtgg tatttactca
     6181 tagcaaaacc catattctac ttatttatta attagcccat atacattagt cagccaaata
     6241 tttttgcgca agctcagaaa actttgactg gtcttttatc agatattctt cgtaaagttt
     6301 ggggttccag atttcaaccc tgttacccat ccctactaca attgcatgct tttcaatttg
     6361 ggcatgcctc agcattggct ttggaatcag aaatctccca ttgccatcaa gttccgccat
     6421 tgaattcccc ctgaaaaaat ttctttgcaa agtccggaat tcctcattga attcactcag
     6481 tcctgctact ttcgaaaaaa tcttcttgta ttcaaccagg ggataaagaa cgaggcacgg
     6541 ctcaaaccct cgtctgatga ctatctcgtt ttcagttgct tcaggcagac ttgatttaat
     6601 ctttgctgga agaaccaaac ggcccttcgc gtcaagctta cactcgtatt cgctggtaaa
     6661 aaatggcatt cccgggtatt attaataagg tacctgtcac aaacttgcat attattcatg
     6721 acattttcaa ccacttttaa ccacttttca ccactttttt acattattga gcacaattta
     6781 ttctaaaaat gacccaaaat gggcgtattg ccaggacagg aacgcttaat tgggaagatt
     6841 accggagttg atcccgggtg tatagtttag ctccttttgg tctcaaaatc tgcactttat
     6901 gcatgtgaaa aggaagcctt cttgggaatt tcaggatgcc tggcatgtca gaacactaga
     6961 acacttaagg taacgatata ttaatataca ctggattagg ttgctcttat actttctcaa
     7021 attcaatatc tttactatac tcatatataa atgggaagaa ataattaaat gccttagata
     7081 cggagttcca aacatcagtg taataccacg attcccatcc agagccctca acatatccta
     7141 atctaatttc tgaccttaca tatatagtct cgctcctaaa actaacaatg aatttatgat
     7201 gaatttgaaa atcagcttcc tttgaatttg ttaccaacaa gagtagttct gaatccctac
     7261 tttcaaatga aaaccctttt gataccagat gccttgaaaa atcgtcaaaa ttctcctgat
     7321 cagacttcga ggtattaact ttaataacat tgtctccttt aacagggtaa tcattactgt
     7381 ccaattgata agtaaatttg taggtaccac aagcactcaa taacataatt agaggtacca
     7441 atttcttcac taaatctgac actttaatca tctttcttgt cacgcttagg tggcggaacg
     7501 ttaaggagtt ttttgagagt ttcatcaaaa tccttttctt catctggggt tttttgaatt
     7561 agctcttttc ttatttctgg attatccaac tctttataat ttttatgagt tactaccttt
     7621 ttgcctgtct gtttttcaat cttcttgata gcctccttag tgatattgcc agcaatatga
     7681 attccattct tagtttcgat aaatccttcc gctccagtct tttcaattat ttccgttgaa
     7741 gttgcttcac tgaaaatcgt tgaagccaat tcgaggtgtc atattatctc tgaggctatc
     7801 cttttttgat aatcccttat gctttttgta ttcagttgtt gaaaaaccaa aagaagattt
     7861 atatagttcg tcagtcaata ttgcatattc atagctttct tttatcccac tatcttttaa
     7921 agaatcagta aaagtatgtc tggtatctat tcccgttaat ctggtttgaa cccatttcgg
     7981 gatataacct ttggctaaat aataaccttt tgccctttct atggcttttg ctggttgttc
     8041 tatttcatct aacctttctt tgccaaccct tgcaagccat cttttaaatg gctccgcttt
     8101 tttggatggc acctcttgaa ttatcctaaa aaccccttca gtactggcac aattaacatt
     8161 ctgtgtgcct cccttggtct tgattggaag ggtatggaca aattgtcccc acccttcacg
     8221 tagggattca tttcttttcc ttaacttatt tatatagtct tttgggttta cagagtctgt
     8281 tagcactcta ataacatctt caatggaaaa ataccattcc ccatctactg caattcttct
     8341 tatttcttca ccttcaaaaa ttgcaatctt tggatttccc tttttaattt tcttactcat
     8401 ttgctaatta atgctgcatg ctttgaacgt cccttcactc ttacttaaaa ttaaattaaa
     8461 tctttcatct ccataagctt gtccggtatt ttagcttgaa attgttcaaa aagactttga
     8521 tgttgaagaa gtcctcgaaa gtgaatatca tttcgtgcgt tcaatagtaa actgcagcag
     8581 gtggatcagg gaagttttgt attcagattc ccggatatct ccgatcagag acagggcttc
     8641 ctggtgaaat ttccccatca cgcttttggc ataatcgatc ccgccctttt ggttaacaaa
     8701 atcaatcact tccttaagct ttttcttatc ctcgctttgg ttcttaacaa tgttgataat
     8761 tcttcttttg tctgaccaac ttgattgcag taatgagtaa attaatggaa gagtcatttt
     8821 cttttctttc agatcgattc ctaccggctt tccaatttca ttttctccgt aatcaaataa
     8881 atcatctttt atctgaaatg ccattccaac cttttcaccg aactttcgca ttaactccac
     8941 ttgctcccca ttctgaccgg cagatgatga gccaaccgca caacaggaag caatcagtga
     9001 agcggttttt tgcctgatga tgtcatagta aacttcttcg gtaatatcca ggttccgcgc
     9061 cttttcaatt tgtagcaatt caccttcact catttcacga acagcgttgg atacaattcg
     9121 caaaatttca tgatcgttat tatctacgga caatagcagg ccacgactta acaagtaatc
     9181 acccacaagt acagctattt tatttttcca aagagcattg atggaaaaga atcctcttct
     9241 gaaattagac tcatccacga catcatcatg tacaagtgta gccgtgtgca atagttctat
     9301 aagcgaagct ccgcggtagg tagactccgt aatgcccccg cacattccgg cagttaaaaa
     9361 aacaaacatg ggtctcatct gcttcccctt ccttctaaca atgtatccca taatcctgtc
     9421 ca
//
LOCUS       DASWHX010000063         2820 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_45612, whole genome shotgun sequence.
ACCESSION   DASWHX010000063 DASWHX010000000
VERSION     DASWHX010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 2820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2820
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45612"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..863)
                     /locus_tag="VGA21_04425"
     CDS             complement(<1..863)
                     /locus_tag="VGA21_04425"
                     /inference="COORDINATES: protein motif:HMM:NF024869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HEX9649781.1"
                     /translation="MRDLVEGIKKWERSNKQFCIATVIETWHSAPRPRGSSMIISSNR
                     EMMGSVSGGCVEKSVVEATKEIFENSKPKLLAYGVVDEDAWRVGLSCGGRIEVFVEKF
                     PSIISEGEKKLWQKLEHKLRSNEAVVWVSEINNEISKHYLVEAAGHNNKNLIHDEAIK
                     LLERGTSQIFEIDDKRYFAQVFSKRKRLLMIGSAHITSELLRLANIYDFETIVIDPRD
                     TFATKTEQVVKPDRLIVKWPQEVLENLYPDKNTFAVILSHDPKIDDAALEILLKKEIP
                     YIGALGSKKTH"
     gene            960..1232
                     /locus_tag="VGA21_04430"
     CDS             960..1232
                     /locus_tag="VGA21_04430"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HEX9649782.1"
                     /translation="MAKVRFTSVLKNFFPEIKEENIDATDVSDLIVKINNKYPGISDY
                     LLDESGKLRGHVCLYIRDEVIPRDKKPELILKPNDQVLIYQAISGG"
     gene            1236..2294
                     /locus_tag="VGA21_04435"
     CDS             1236..2294
                     /locus_tag="VGA21_04435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEX9649783.1"
                     /translation="MKLLACTDKGLIEFENVSGKFQFGRIHFSGQPVAYLDCYDEVWR
                     VAINHKHWGSKIHKSEDSGQSWQELNAPKFSSILSQNNQATLRSIWNITAHNKEEFYV
                     GVEPAAIFHSRDGGNSYDFMEGLWNHPTRKSWIGGGKGSTKPFLHHIIVDPANHNHLF
                     AAVSCAGVLESYDNGKSWKMINEGIRADFLPNPEAEAGHDPHNLKMSQKNPLVIWQQN
                     HCGIFRTENGGKHWNDLTPNIETSGYGFALAIDAKDDNRAWVIPCESDSMRIAPGNKL
                     SVYSTSDAGTTWRRLSNGLPQDGVFDIVLRHGLDIQNSHLAFGTNNGNLYHSPDFGEN
                     WNIITQNLSAVRCVKLLA"
     gene            complement(2286..2717)
                     /locus_tag="VGA21_04440"
     CDS             complement(2286..2717)
                     /locus_tag="VGA21_04440"
                     /inference="COORDINATES: protein motif:HMM:NF013738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HEX9649784.1"
                     /translation="MSRIENLYQEKIMELAKSSIHFFELKTPSHVFTAYNPLCGDKFT
                     FYLLAENGKIKEASFSGFGCAISKASSSLLVSKLIDNELTNLSMLVNDFLITIDSSSR
                     GKIPDVVYAPFLAVREFPERISCVTLGWKELENQLKQNNLS"
BASE COUNT          843 a          562 c          555 g          860 t
ORIGIN      
        1 tcatgggttt ttttgctgcc caaagctccg atatatggaa tttctttttt cagcaaaatc
       61 tctaatgctg catcgtcaat tttagggtca tgacttagaa ttacggcaaa agtattttta
      121 tccggataca gattttccag gacttcctgc ggccacttaa caattagccg gtctggtttt
      181 actacctgct cggtttttgt agcaaaagtg tcgcgagggt ctatgactat ggtttcaaag
      241 tcataaatat ttgccaatct caggagttct gaagtaatat gagccgagcc gatcatcagc
      301 agcctttttc ttttggaaaa tacctgggca aaatatcttt tgtcatcgat ttcaaaaatt
      361 tgacttgtgc cacgttccag caatttaatg gcttcatcgt gaatcaggtt tttattatta
      421 tgcccggcag cttccaccag gtaatgcttc gatatttcat tattgatttc tgaaacccaa
      481 accacagctt cattggaacg aagtttatgt tccagttttt gccacaactt tttctcccct
      541 tcagaaataa tggatgggaa tttttctacg aaaacttcta ttctccctcc acaacttaat
      601 cctaccctcc atgcatcttc atcgaccaca ccataagcca acaactttgg cttgctgttt
      661 tcaaatatct ctttggtcgc ctctactacg cttttctcaa cgcatccgcc actaactgac
      721 cccatcattt ctctgtttga agaaataatc atcgaagagc cccgcggtcg tggcgcggaa
      781 tgccaggttt caattactgt cgcgatacag aattgcttat tgctgcgctc ccattttttt
      841 attccttcaa ctaaatctct caaagtttaa tattttcctg aaatttactg attaacaaaa
      901 tatttaatga atattcgaca attaattatg aaatataata atatagtata ttagcaataa
      961 tggcgaaggt taggtttact tccgtattaa aaaatttctt tcctgaaatc aaggaagaaa
     1021 atattgatgc gactgatgtt tcggatttga tagtaaagat taacaataaa tatccaggca
     1081 ttagcgatta cctgcttgat gaaagcggaa aactaagagg ccatgtttgt ttatatatac
     1141 gtgatgaggt aattccacga gacaagaaac cggagttaat tctgaagcca aatgaccagg
     1201 tcctgattta ccaggcaatt tccggagggt aacttatgaa acttttggct tgtacggata
     1261 aaggcctgat tgaatttgaa aatgtatctg ggaaatttca atttggaaga atacatttct
     1321 caggacaacc ggttgcatat cttgattgtt atgatgaggt atggcgtgtg gccatcaacc
     1381 ataaacactg gggttccaaa attcataaga gtgaagacag tggccaatca tggcaggagt
     1441 tgaatgctcc gaaattttct tccatacttt ctcaaaataa tcaggccaca ctccgatcaa
     1501 tatggaatat caccgctcat aataaggagg aattttatgt tggggttgaa ccagcggcaa
     1561 tttttcatag tcgtgatggc ggtaattctt atgatttcat ggagggttta tggaatcatc
     1621 ctacaaggaa atcctggatt gggggcggca aaggttcaac aaaacctttc ctgcatcaca
     1681 tcattgtaga tcctgcaaac cataaccacc tttttgcagc ggttagttgc gcgggcgtac
     1741 tggaaagtta tgacaatgga aaatcatgga aaatgattaa tgaaggaatc agagcggatt
     1801 ttcttccaaa tcctgaagca gaggcaggtc atgacccgca caatctgaaa atgtcgcaga
     1861 aaaatccatt ggttatttgg cagcagaatc actgcggaat ttttcgaaca gaaaacggtg
     1921 gaaagcattg gaacgatctc acgccaaaca tagaaacttc aggctatgga tttgcccttg
     1981 ccattgatgc caaagatgat aatagagcct gggtaattcc atgcgaaagc gatagtatga
     2041 gaattgcccc gggcaataaa ctttcggttt attcaacttc agatgccgga actacctgga
     2101 gacgcctttc caatggtttg ccacaggatg gcgtgtttga tattgtgctg cgccatggat
     2161 tggatataca gaattcccac ctggcgtttg gaaccaataa tggaaactta tatcattccc
     2221 cggactttgg agaaaattgg aatataatta ctcagaattt aagtgccgtg aggtgcgtaa
     2281 aactactagc ttaggttatt ttgtttcagc tgattttcca attccttcca acctaaagtg
     2341 acacagctaa ttcgttcggg aaattccctg acagccagaa atggagcata gacaacgtcc
     2401 ggaattttac cacgcgaaga tgaatcaata gttataagaa aatcattcac aagcatggaa
     2461 aggtttgtca attcgttgtc aattaattta gaaaccagga gcgacgatga agctttagag
     2521 atggcgcacc caaagccgct gaaggacgcc tcctttattt tcccgttttc tgctagaagg
     2581 taaaatgtaa acttatcacc gcataatggg ttatatgcag taaagacgtg cgaaggagtt
     2641 ttcaattcaa aaaaatggat tgagcttttc gccaattcca ttatcttttc ctgataaaga
     2701 ttttcaattc tgctcatgta aaaaaacttt ctgccttttc caaaacggaa accaatttat
     2761 ctacttcgtc ttttgtgtta taaatagcaa atgaaattct gcaggtaccc tgaatattta
//
LOCUS       DASWHX010000064         2273 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_3009817, whole genome shotgun sequence.
ACCESSION   DASWHX010000064 DASWHX010000000
VERSION     DASWHX010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 2273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2273
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3009817"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1722)
                     /locus_tag="VGA21_04445"
     CDS             complement(<1..1722)
                     /locus_tag="VGA21_04445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649785.1"
                     /translation="MKQFLITIALLSFASLCLGQTKKALKEIEKNEWQKAEQALIKGL
                     QKDSLDALSHHVYSILLSNGWAPQFDIVKSSEYNKKAQKLFSNLDEKELSKLEKSDLS
                     QRVLESQKKFIDSVAFILALQKGTEQGYINFIELFPDARQIHQAIKARDRIAFTETQN
                     TNTITSYRNFLNKYPDADQVPKAQEIFDKLLYEEQAKNADLEKMISFLKEYPDNPYNQ
                     EIEFNVFKQISGFFSEENLFQFIETLPESKYAQLAYEFLYHLKKDNRTRWEFLTQNSK
                     YSKADSLRTLLENESGFLIPIIENQRVGFINSQGNMVISPDFQDPNNALKCSLISGDL
                     IQVKNGSQISLFNRSKDLVFQTQADSVKDLGNGFIRISVHGRQGLIHKNGYVFFEPSF
                     RDIELLSNSIFKFRRAEGWGLMDPFQKIIFSDNLTSITGYGKIAALEKNGKLGLFNIE
                     ELYNQTNAQPSQFPFDDLEQLPGGQFLCFNGSKEVLLDENLKEIIPAATQEIIHFKNY
                     WISKGEVATKLFSNSGKILIDSIQSYSIYGSKLGYKRNGRWRIFETAESKNTEFDSLK
                     ILSDNVYI"
     gene            1934..2008
                     /locus_tag="VGA21_04450"
     tRNA            1934..2008
                     /locus_tag="VGA21_04450"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1969..1971,aa:Pro,seq:agg)
BASE COUNT          645 a          422 c          445 g          761 t
ORIGIN      
        1 tatatataca ttatcgctta aaatttttaa agaatcgaat tccgtattct ttgattcggc
       61 agtttcaaat atcctccacc tgccattgcg tttgtatccg agctttgatc cataaataga
      121 atagctctga attgagtcta tgagaatttt tcctgaattg gaaaataatt ttgttgcgac
      181 ttcacctttt gaaatccaat aattcttaaa gtgaataatc tcctgagttg cggcaggaat
      241 tatttctttc agattctcgt ccagtaaaac ttccttgcta ccattaaaac ataagaattg
      301 cccgccaggt aattgttcta aatcatcaaa tgggaattgg gaaggctgtg cgttggtttg
      361 gttgtaaagc tcttcgatat tgaaaagccc aagctttccg tttttttcca gggcagcaat
      421 tttaccatat cctgtaattg aagtcaggtt atcactaaag atgattttct ggaatggatc
      481 catcaagccc catccttcag ctcttctaaa tttgaaaatg gagtttgata aaagttcaat
      541 atccctaaag gagggctcaa agaagacata cccatttttg tggattaatc cctgtctgcc
      601 atgcacggaa atccgaataa atccatttcc caaatcttta acgctatctg cctgagtttg
      661 aaaaacaaga tccttgctcc tgttgaacag gctgatttgg cttccgtttt taacctggat
      721 caaatcacct gaaatcaagc tgcactttaa cgcattattg gggtcttgaa agtccggaga
      781 gataaccatg ttaccctggc tgttaataaa tccgaccctc tgattctcta taataggaat
      841 cagaaatccg gattcattct ctaataatgt ccgcaatgaa tctgctttgg aatatttcga
      901 attttgcgtt aaaaattccc aacgagtccg attgtctttt tttaagtgat aaaggaactc
      961 gtacgctaat tgagcatatt tactttcagg taaagtttct ataaactgaa atagattttc
     1021 ttcactaaag aatcctgaaa tttgtttaaa tacattgaat tcaatttcct ggttgtatgg
     1081 gttatcagga tattccttca gaaaggaaat cattttttcc aggtcggcgt ttttagcctg
     1141 ttcttcatac aggagtttat caaagatttc ctgtgccttt ggaacctggt ctgcatccgg
     1201 gtatttattg agaaaatttc ggtatgaagt tatggtgttt gtgttctgag tttcggtgaa
     1261 ggcgatgcga tcccttgcct ttatggcctg atgaatttgc cgggcgtcag gaaacaattc
     1321 aatgaagtta atgtatccct gttcggttcc tttttgcaat gctaaaataa aagctacgct
     1381 atcaataaac tttttctgag actcaaggac acgctggctg aggtctgatt tttcgagctt
     1441 tgacaactcc ttttcatcca gattgctaaa taatttttgc gccttcttat tatattctga
     1501 ggacttaaca atatcgaatt gtggcgccca tccattagat agaagaatag aatatacatg
     1561 atgcgaaaga gcgtcaagag aatctttttg cagtcccttt atcaaagctt gctctgcttt
     1621 ttgccattca tttttttcta tttccttgag cgctttcttt gtctggccaa gacacaaaga
     1681 agcgaatgat agaagcgcta tggtgattaa aaattgcttc atgggggaac aaatttatta
     1741 atttttgact gggaaatgac tgctacctaa tattaacctg ataaaatgaa attttattgc
     1801 ccaaaatgag cggataggga aacttgcgaa atgtgagtct taaaaaatgg ccggaaatga
     1861 ttaatattgc aactcgattt ttaataaggc aatttgattt ttttgtcatt ttgaaattga
     1921 attattaata actcgggatg tggcgcagcc cggttagcgc acctcgttag ggacgagggg
     1981 gccgctggtt caaatccagt catcccgact tgttattgtt gaaggaactt tgatttaata
     2041 aatggatcaa ggggaaaggc gcacctcgtt agggatcccg gcaggccggg actggttcaa
     2101 atccagtcat cccgacttga tattgttcat ggactttgat ttaaagaaag gagaattgat
     2161 aatgagtttg tctatcttct tcatttattc atttctaatc attgctctgt aggagcattc
     2221 tgtttgtagt tatcgcagaa tacaacaacc gtaagctccg taggagcgac ctg
//
LOCUS       DASWHX010000065        22344 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_2634394, whole genome shotgun sequence.
ACCESSION   DASWHX010000065 DASWHX010000000
VERSION     DASWHX010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 22344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22344
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2634394"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(67..2445)
                     /locus_tag="VGA21_04455"
     CDS             complement(67..2445)
                     /locus_tag="VGA21_04455"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9649786.1"
                     /translation="MLKNYITTAIRNLAKNRIYTLINVSGLTVGIAAVMFILIYVNYE
                     TGYDVQFNNDNKVYRLIRNFNTSTGSRDVGITSGPYATALLNDFPGRIKETLRVTSSS
                     SLITYDEKSFMEEKVFFADSNFFNFFKIPVIKGSTEKALLGNSILISSSMSAKYFGDT
                     NPVNEILQIDNEYLFTITGVFDETRIQSHLQFDFLGSLYYAQNFKYFDDWWSNDFSTY
                     LMLDEGAQIGEINAGLPAFTEKYFGDDWKRGVGNGLSLKTEALEDIYFNNETSFDFVL
                     HGNKKVVYLFSGIAAIVLLIAAFNFVNLSTALSVSRYVEIGIRKASGASRNQIIIQFL
                     MESILLVSCSTLLAFLALETLYPLFLSVVGKAITNPITIELGILLALFVSIVIGILSG
                     LYPAIILSGFSTGKVLKGKPDSGHRGQVLRRGMVVLQFTFSIFLIFATWITWDQLKFV
                     QSKNLGFSYEDRLFFPINNNEFQQKKEVFKQNVLKLPGVESLTFATGEPGGFHDFNVF
                     NVEGQEGTVSMRTAFTDLDYLTTLDLNLIAGRNFSRDFPSDLTDAIILNEEALKTLDW
                     TPEDAIGKKIENIFRDTIPRTVIGVVNNYHFRSLKEKINPLVISITSNHYVGIIKFKT
                     KDLQKLVHDLTGEWNLYADGFPFAFNFLDDRIEQLYLDEKNQSQLFLIFCSLAIFIAC
                     MGLFALSILAAEKRMKEMAIRKVHGARVSQIVAKLNFEFIILVLISTVIMIPFGWYLM
                     DKWLQNFEYHIEIGWIYFMGTALFVVILSLITVSYKAFRVAGLNPAIVLRYE"
     gene            complement(2738..3127)
                     /locus_tag="VGA21_04460"
     CDS             complement(2738..3127)
                     /locus_tag="VGA21_04460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649787.1"
                     /translation="MLKYSRILFWLPRIMTILAILFVSIFALDAFQPELTVTEQWGGF
                     VIHLVPSYLLTVILIVAWKWEYVGGILFLVIGLLATPYVFLGNYQVNNSLGQSILVVL
                     SVTIPFAIVGLLFLVNHFRNKKFQSAK"
     gene            3384..3848
                     /locus_tag="VGA21_04465"
     CDS             3384..3848
                     /locus_tag="VGA21_04465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649788.1"
                     /translation="MLDFRTAYYFPWTMIIISPFLVLFGGVLIVEQIYLGVLLVVTSL
                     LFLTTHYRFKIDEKRKEYREYLWILGFKHGKTIAYEKVISLYFTKSRMKQTINSRVSS
                     MTVSNEVYNGFIQFDGDKKVHLFTKGKKQKLVDCLISISKFLKTEVKDLSGS"
     gene            complement(3988..4935)
                     /locus_tag="VGA21_04470"
     CDS             complement(3988..4935)
                     /locus_tag="VGA21_04470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX9649789.1"
                     /translation="MRSLILFCAFAFLMSACGTSTSDESAETPDSTTVAKYSYSNSFE
                     MGDPAYLTLVRNWNDALEAGNIDEAFSYLADSIHVILANGDRFDTTKDSLRVVVDQML
                     ATMSDIKIAYIAGASVRSLDRNESWVLSVTDESMTNEEGENRVIAHELYRIEEGKIRS
                     IYAYAQIPSEADQIPDYTSDEYLYSGSFEMGNSENEVVVDDFLKAFETLDFSEIGNYF
                     ADSVTLFFADGFFRNTVRDTVIADIMRYVENKQITLNRHASMALRSVDLNEEWVLVWI
                     DETTSGPDGEENYILHEAYKLVNGKIRVMRQSAREYVEE"
     gene            complement(5135..5836)
                     /locus_tag="VGA21_04475"
     CDS             complement(5135..5836)
                     /locus_tag="VGA21_04475"
                     /inference="COORDINATES: protein motif:HMM:NF024965.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEX9649790.1"
                     /translation="MRKIYVIILATLSTVTAFAQQRVNAREILREIDEGKSVSFKDVE
                     IEGNLDFTDLRNRRHKDSSFSWFGNSNDSYESTVEASVTFINCTFLDDVIAYYHLERN
                     DDTFIAHFDDDVVFKNCTFKRKSEFKYSEFAENVDFSGSVFNREANFKYAEFSELPFF
                     NDAEFRDDANFKYAEFPNGANFESATFLELANFKYTKFRTPVNFKNISFEGYEDFKYT
                     KVDGRSFTSYLLDNR"
     gene            6026..6721
                     /locus_tag="VGA21_04480"
     CDS             6026..6721
                     /locus_tag="VGA21_04480"
                     /inference="COORDINATES: protein motif:HMM:NF014724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEX9649791.1"
                     /translation="MRNNIHLVIHYLIFIACILAISGCKYYYGAFYTDPQKCFGDALR
                     EKPYDIVIVPGFPSDSGKINTTLSERILWADYLYKNGIANQILFSGSAVYTPFIEGEI
                     MRLFALQLGIDENEIKVETKALHSTENLYYGFKMASESGYEKIAFATHPAQSSFLKQF
                     RRKFNLDLDFIPIVADSIKNIKYSFQSIDLSKIFVEDFIPLNERQGLFKKLRGTRGGR
                     VKKEIRKEKRSND"
     gene            6879..9251
                     /locus_tag="VGA21_04485"
     CDS             6879..9251
                     /locus_tag="VGA21_04485"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX9649792.1"
                     /translation="MIKNYLTISFRHLLKNKVYVIINAVGTGIAIACGLTAYLLIGYN
                     YEFDDYFNGKKAQNIVKVMHHFKTSGGNSDQELAIPVGLSERVFQEVSGIENVTRFCN
                     QHGILSSEHDSFFENIRFADASFFDMFHPELKYGSYKNFKARETVYLTEPIAKKHFGD
                     IDPTGKPVTIEINERLYEVLVGGVFEKLPLNISFTLDVLLRIELYFDANSISSNSLDH
                     YTSALFSLTDSKNTRFAGEQLNIYLSEINASDKERISTSFELIPFERKVINGEVSASD
                     LRLPIPTMALLVFGILATIILLIACFNFTNTTLALTNTRFKEIGVRKVVGARKSGIVI
                     QSLIETTLTILVAISLGVLMAQIIVPRFATMWRLEFGLGDLSTTNFIMIIGLLVMAIV
                     TLAGIYPAFQNSNLKPIELLKGKRKIRGTNIFSQTFLVLQFSLSVIMLIAGVVFTQNA
                     QYQKELDFGYDYKDVIAITLYGEDDFTRFKNQISGFSQIKEIAGAKNPIGPYTALKGR
                     FTIDTTQFTSDLHFIGDNYFNAIGLNLSRGRNFNAEMISDRESSLIVDENFVSNHNLA
                     NPVDARVIFDEKPYRIIGVVENHLSGLKKERKTEHVFALAVNTDFKSMLIKTIGGENL
                     AIFTYLEKEWRKINPAKPFLGIMQEEILFEEANGYNNNLTQIFFFLTTLGSLLSAFGI
                     YALAKLNVQLRFREIGIRKTLGSGVSNIIKLLNREFALILTIAAIAGGFIGYFMTNAL
                     LDDLYTYHLEISIFTLLVSGVSIFIIGIISTSSTIFRAANTNPIEILKDE"
     gene            9709..10287
                     /locus_tag="VGA21_04490"
     CDS             9709..10287
                     /locus_tag="VGA21_04490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649793.1"
                     /translation="MDDLPVKVALPLFRKEFNLDIPGGDDEIVRIVIGRIVLYIPNFE
                     TRKKIVLLHDIHHLATGYSAFFKGETEISAWELSAGCYNNWVAFIINIYAFMIGLIIN
                     PTKVWRAWVRGRNTRTLYFMGYHYDELLQKTVGAIKKELGLMSGINHRRLLKTIVSFI
                     LMIIIGLAVSILSILLVPLTIFFTLYIRLKSG"
     gene            10284..11825
                     /locus_tag="VGA21_04495"
     CDS             10284..11825
                     /locus_tag="VGA21_04495"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEX9649794.1"
                     /translation="MKKLKTDVLIVGAGPTGLMAANQLQRFGIDFMIIDKKSGPTMES
                     RAIAVTARSLEIYEQMGLSDTAIQEGQRIEIFNLYVKGKPRVSVKIGEIGKGLSDFSY
                     MLAFEQSKNEVLLSRNLYNHGKSIHWNYEFVEIKENQDNVTVLARNPDIEVEITALYL
                     IACDGAGSPIRQHLNFSFRGGTYKHKFFVADAILKWSIPYDQLIISPGTENFCAFLPL
                     QGNHTYRIIGTLPKSYFNTETVTFHDIREVVIRTLGIEIEFEQVNWFSTYKLHHRGVN
                     KFDNNRVFLAGDAAHIHSPAGGQGMNTGLQDAYNLCWKLALVLKGQAFESLLKTYNEE
                     RLPFAKWLLRFTDRGFNMMTNRNFFIRKFRELIAINLVKWVPRVGFIRPVIFKVLSQI
                     WYSYHGFSLAQSSTRQNLKFVSGDRLPYFPGEKFYSLFKNPSFHLLCLTNTSISDIQK
                     QNLQDSFPFHIKIIEQKINEMWNKLGVVSELYIVVRPDNYILFIADSIHKNILEHYFK
                     DFPKC"
     gene            12237..13268
                     /locus_tag="VGA21_04500"
     CDS             12237..13268
                     /locus_tag="VGA21_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018474467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX9649795.1"
                     /translation="MRSIQITIKDLAKELGISPSTVSRALKDHPDISKETKRQVNELA
                     ERLNYQPNNIALSLRQRKTNTIGVVIPEIIHFFFSTVISGIEDIAYKAGYSVIIAQSN
                     ESYDRELKDTKALFNGKVDGMLISISRETSDFGHFESLMERGLPLVFFDRVCDAIITD
                     KIIIDDKEAADQATQHLIDQGCRRILHLSGPKSLLIGQQRLGGYLQALERNKIPVSDE
                     LIIDCGHGTEIEAAQKVHEFLQKYPAFDGVFATNDVAAMGAMMALKEKNVKIPEEVAV
                     IGFGNWRFGALTDPPLSTVEQPGYEMGCEAAKHLIRQIELKDDESFEATTKILKTRVI
                     PRKSSLRNS"
     gene            13365..15803
                     /locus_tag="VGA21_04505"
     CDS             13365..15803
                     /locus_tag="VGA21_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014773787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HEX9649796.1"
                     /translation="MQKTITRNKTLNDGDHYRSLGTLQSYSKTNLGIEGSTSHGSFII
                     TFYNESIVRIRVCEEDFEEDFSYSVIAIPENPEFIVEEINGEIILSTGKLVVKIMKDP
                     LRISFLNLQGSVICADDKAFGTSWLGTQVTTYKELQKGERFIGLGEKTGPLDKQGKGY
                     ENWNTDAFGYGVDTDPIYSSIPFYIGIHHNLQYGIFFDNSFKSHFNFGASNNRFSSFS
                     ADGGEMNYYFIYGETITDVIFQYTKLTGRIELPPIWSLGYQQCRYSYSSEEEVLRIAD
                     TFRMKDIPADAIVLDIHHMDKYKIFTWNPATFKNPEKLIAKLKQKGFHAVVICDPGIK
                     ISADYHAYKKGIENDIFLKYPDGALYEAQVWPGWCHFPDFSKAECRELWSQLLQHYTQ
                     AGVTGFWNDMNELATWGQMMPEMIQFDYEGKKASLRRMKNLYGLLMAGATQTAARKQL
                     NGKRPFNLSRSGFAGIQRFAALWTGDNTSSEEHMLLGVRMVNNLGISGVPFTGYDVGG
                     FTSDASPELFARWISVGAFSPFFRGHSVINSRSAEPWTFGERVLEISRNYIKLRYKLL
                     PYIYSSFYEASINGLPVCRSLVINYPHEAKIFHQDFQNQYLFGQNIMVAPAPGNQELS
                     RVFLPEGLWYDLFTDKVFNGTEEIIVESPLEKLPLFVKGSGILFLQSEIASTTQKPDD
                     TLEVHIYNGTLESYFTYYEDDGDSYQYLENEFFRRMIRFNPENRSICFEKSEGELNSK
                     FKTLRVFLHGMKNIDKINVDGKILELQVRDFQFIRPISCFDPIHSGKDYEITINNLPY
                     FDLPNNGGKTEIHY"
     gene            15818..17185
                     /locus_tag="VGA21_04510"
     CDS             15818..17185
                     /locus_tag="VGA21_04510"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEX9649797.1"
                     /translation="MKSIKQIVFPAFIVFLAFSFSCTAPTKEKEPVEITSTYPDKIKN
                     LTIYEVNVRQMTPEGTFKAFETHLPRLKKMGVGILWFMPIHPIGIENRKGSLGSYYSV
                     RDYRGVNPEFGTIEDFKNLLEKSHEMGFEVIIDWVGNHTSWDHAWMAEKPDFYTRDST
                     GNVIPPVADWSDVADLNYDNKELWDYMISDMKYWTNEIGIDGFRCDAAPWLPGEFWVK
                     AIQELEAEKDLLMLAEGEGPEWHEAGFDMTYAWEYHHIMNGIVRGEKNVVDLAEYMEK
                     ELSSYPNGGYRLIFTSNHDENSWAGTVKERFGDAVKTFAALSFTYYGYPLIYSGQEAG
                     MDKRLRFFDKDTISWEDLSMQSFYHELIQLRKDDPAIWNGDEGGNPEIINIDNLENVL
                     AFSRISGTNSVIGFFNLSDTEQSFSVKESVIGDYVDLISDNQYYINGQAPITLPPWGF
                     MILSN"
     gene            17189..18577
                     /locus_tag="VGA21_04515"
     CDS             17189..18577
                     /locus_tag="VGA21_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009578086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9649798.1"
                     /translation="MEEKLHAEQELSLSSEKPALSFWQIWNMSFGFLGIQFGFALQNA
                     NVSRIFETLGASQDELPILWLAAPVTGLLVQPIIGYFSDRTWHPRWGRRRPFFAVGAI
                     LASLALCIMPNAGLLWIAAGTLWLMDASINISMEPFRAFVGDMLPGHQRTRGFAMQSF
                     FIGIGAVVASMLPYILTNWLGVSNTAPAGEIPNSVKWSFYMGAIAFFSAVMWTVSRTK
                     EYPPDDLEKLKADNKNLSSVQILRESFEGIFQMPKTMAQLAVVQFFSWFALFSMWIYT
                     TSAVTKHIYGTSDTSSALYNEGADWVGIMFATYNGVAALTALMLPWLAKVTSRKITHL
                     ICLCCGGLGLLSFYMIKDPNLLIMSMVGVGIAWASILSVPYAMLSSSLPAKKMGYYMG
                     VFNFFIVIPQIVAAGILGFMLRNFLDGAAINAIIVGGASMILAGFLSLIVDDKEEVHL
                     SEEIPGTHKPES"
     gene            18866..19069
                     /locus_tag="VGA21_04520"
     CDS             18866..19069
                     /locus_tag="VGA21_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018620089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoisovalerate dehydrogenase"
                     /protein_id="HEX9649799.1"
                     /translation="MSEIIFIVEESAEGGFEAKALGESIFTEAESMEELKENIREAIR
                     CHYDENAPKVVRLHYVKEEVFAA"
     gene            19329..19493
                     /locus_tag="VGA21_04525"
     CDS             19329..19493
                     /locus_tag="VGA21_04525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649800.1"
                     /translation="MNEAGYAWDFHPRKNLKPFPNLYTYFQVMFFYEFNSHLYLTSFW
                     DIFGFQELEE"
     gene            19613..19810
                     /locus_tag="VGA21_04530"
     CDS             19613..19810
                     /locus_tag="VGA21_04530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649801.1"
                     /translation="MRNNLAPAKALIATYSFDPLIGMTSQTDTNSQTKWFYYDSNGRL
                     SLIKDQNGDIVTQHEYNFVVK"
     gene            19932..21473
                     /locus_tag="VGA21_04535"
     CDS             19932..21473
                     /locus_tag="VGA21_04535"
                     /inference="COORDINATES: protein motif:HMM:NF037015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEX9649802.1"
                     /translation="MCKLIFSRAFFIIPFLLLAVASSCRNETMDDTDPTVSITNLMEN
                     QKVRGKITVNFSPMDEHLDKVYFFIDNVSLKTLTHSPWIMDIDTEDFEDGNHAIKVIA
                     ADANSNSSTAEVNVEFKNLMLTVEIGNSFEEGQNSYIIIYDMEGDAVDYRLIENNSTF
                     KFPYPDDYEGWDFNATYFTVNNDNFGQSFYSETIQRCSAPRWVFPDLTDDEDYYEQNI
                     SLINVPDHDEIYASNGFQYNGIGSNNTTKLYTLVEPDNLFVGLIEGQAARYKWIPQVA
                     AGSIEIDLTAMQEMLTGSVSAQVPVSSLYVKGNINNPGGLLSYFLSSSTNEHFFYADV
                     FDTYSVSLRSVMPNLKTLYQVITDDLPISFQAPSFDANLTSANQEIFNFNTAGDFDYF
                     SVYWYHYDYDPMTNYQSAHFETFGGPGDEAKYFPLPEVLKNVYPDFDPSLLVPGGISF
                     QENNVLSGYAHFSFLYNTDQNYPVPTRIDAYSIEPDPNGGRVSKPGYVKVKRWGKSRR
                     FNQTF"
     gene            21704..21907
                     /locus_tag="VGA21_04540"
     CDS             21704..21907
                     /locus_tag="VGA21_04540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649803.1"
                     /translation="MNSMKGITFITDETHKKRFVQIDLEQLEKYQHEIEDLLDVIIAE
                     SRKNDEQVSWEEIKAQLKAEGKL"
     gene            21907..22194
                     /locus_tag="VGA21_04545"
     CDS             21907..22194
                     /locus_tag="VGA21_04545"
                     /inference="COORDINATES: protein motif:HMM:TIGR02385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEX9649804.1"
                     /translation="MYQITFRKKAGKELLRLSSQIVKKAAKSIDSLSKNPRPKGSKKL
                     KASDENMWRIRIGDYRVLYTIDDQVKIIDIRKVGHRKDRGCSTNCVKIKNY"
BASE COUNT         7037 a         4245 c         4391 g         6671 t
ORIGIN      
        1 ccctgtagtc ccacttaaaa caaggggaca atttcctggg gaatttacac tttagaaaat
       61 ttcagatcat tcgtaacgaa gcacaattgc cggatttaag ccagccaccc taaaagcttt
      121 gtagctcact gtaattaaag aaaggatgac tacaaagaga gcagtaccca tgaaatatat
      181 ccagccaatt tcaatgtgat attcaaagtt ttgcaaccat ttatccatca ggtaccagcc
      241 aaacggaatc ataataaccg tacttatcaa aaccagaatg ataaattcaa aattaagttt
      301 ggctactatt tgcgaaaccc tggctccatg aacctttctt atcgccattt ctttcatgcg
      361 cttttcagct gcaagaattg acagtgcaaa gagacccatg caggcaatga agatggccag
      421 actacaaaat atcaggaata actgcgattg gtttttctcg tccaggtata attgctcaat
      481 ccggtcatcc aggaagttaa atgcaaaagg aaatccatct gcataaaggt tccattctcc
      541 agttaagtcg tgcactagtt tttgaagatc ttttgtcttg aattttatga tacctacata
      601 atggttggaa gtgatagaaa ttaccagcgg atttattttc tcttttaatg accggaaatg
      661 ataattattt acaacaccaa ttacagtcct gggaatcgta tcacgaaaaa tattttcaat
      721 cttttttcca atagcgtctt caggtgtcca atctaatgtt ttcaatgcct cctcatttaa
      781 gataattgca tcggtcaggt ctgaagggaa atctctgcta aaatttcgtc cggcaattag
      841 attgagatcc agggtagtca ggtaatccag gtctgtgaac gccgttcgca ttgatactgt
      901 cccctcctgc ccttcgacat taaaaacatt gaaatcatga aaaccaccag gctcaccggt
      961 tgcaaacgtc agactttcaa ctcccggcag cttcagcacg ttttgtttga acacttcttt
     1021 cttttgttga aattcgttat tgttaattgg gaagaataac ctgtcttcgt agctaaatcc
     1081 aagatttttg ctttgaacaa attttaattg gtcccaggta atccatgtgg cgaaaatcag
     1141 aaatatcgaa aaagtaaatt gcaaaactac catgcccctt cgcaaaacct gaccccggtg
     1201 tcctgaatcc ggcttacctt ttaagacttt tcctgtagaa aatccggaaa gtataatcgc
     1261 cggataaagc ccggataaaa ttcctataac tatcgaaaca aatagggcta acagtattcc
     1321 taattcaatg gttattgggt tagttatggc tttgccgact actgataaaa acaagggata
     1381 tagagtttca agagccagga aggctagcag ggtggaacaa gatactaaca gaattgactc
     1441 catcagaaat tgtattatta tctgattacg acttgccccg gatgcctttc tgattccaat
     1501 ttcaacatac ctggagactg ataatgccgt acttagattt acgaaattaa atgcagcgat
     1561 gagtaaaact atggctgcaa tccctgaaaa caggtacaca acttttttgt ttccgtgcag
     1621 cacaaagtca aagctggttt cattattaaa ataaatatcc tccagtgctt cggttttcaa
     1681 tgacaaacca tttccaacgc ctcgtttcca gtcatcaccg aaatatttct ctgtaaatgc
     1741 aggcaaaccg gcatttattt ccccgatctg agcaccttca tccaacatta aataagttga
     1801 aaaatcattc gaccaccaat catcaaaata tttgaagttt tgtgcataat aaagtgagcc
     1861 caggaaatca aattgaaggt gagattgaat ccgcgtttca tcaaaaactc cggtgatggt
     1921 aaacaagtat tcattgtcga tttgcagaat ttcatttacc ggattagtat caccaaagta
     1981 ttttgcagac attgatgatg aaatcagaat agaattgccc aaaagagctt tttcagttga
     2041 tcctttgatt acaggtattt tgaagaaatt aaaaaagtta gaatccgcaa aaaatacttt
     2101 ctcctccata aaagattttt cgtcataagt aatcagtgaa gaggaagacg ttactcttaa
     2161 tgtttctttt atgcgtcccg gaaaatcatt aagcagtgca gtggcataag gtccggaagt
     2221 aattcccaca tctctgctcc cggttgaagt attgaaattt cttattagcc ggtaaacttt
     2281 gttatcatta ttaaactgaa catcataccc ggtctcatag tttacataaa ttaaaatgaa
     2341 catgacggcg gcaatgccaa ccgttaagcc agatacattt attaaagtat aaatccggtt
     2401 tttggcaaga tttcggattg cagtggtgat gtagttttta agcatacctt tgaattttag
     2461 tagtggtgtc tcagaacatc aacgtgtaac aacacttcac tttttatttc aggtgaaaat
     2521 tgactgattt tcaatgtcgg gaaaagtgct gcacatgtaa aaggctcgtt tctaagcatg
     2581 cgctctgaat tttcgtgtta aacacacgaa ttatgccagc aagatatttc aagtatattt
     2641 aattagtaat cagatattta tgaagaagtt atccaacaac cagtgtttca ctgtgaaaca
     2701 attgtgtttc cccctgaaca atgatttcgc ttttcgtcta tttagctgat tgaaacttct
     2761 tattcctaaa gtgattcacc agaaagagca gacctacaat agcaaacgga atagtaactg
     2821 acaggacaac caagatactt tgtcccaggg agttgtttac ctgatagttt cccagaaaca
     2881 cataaggagt tgccaataat ccaataacca ggaaaagaat gccgccaacg tattcccatt
     2941 tccaggcaac aatcaggata acggtcagca aataagaagg aacaagatga ataacgaaac
     3001 ctccccattg ctcagtaacg gtcaattcag gttgaaatgc gtccagggca aaaatactta
     3061 caaaaagtat ggccaaaatc gtcataattc taggcagcca gaatagtatg cgagaatatt
     3121 taagcatatt agctcaattt tggttacttc aaagattgta agccgggata aattcaacaa
     3181 tgatatttat caggttcggt aatttgatat gccaaaacta aggtacgaaa gttttatttt
     3241 gatctgtcga atttagcttc tcaaaatccg gaatatttta gttaaatact tcccggcatc
     3301 aaaataataa ccggtctact cggctaaggc atggttggag ttaaaatgtt atttttgcta
     3361 tttattatca aattagattg aaaatgctgg attttagaac cgcttattat tttccctgga
     3421 caatgataat tatttctcct ttcctggtat tattcggcgg agttttgatt gttgagcaaa
     3481 tttatctggg ggttttattg gtggtcacaa gcctcctctt cttaactacg cactaccgat
     3541 tcaaaattga tgaaaaaaga aaagagtacc gcgagtattt atggatttta ggatttaaac
     3601 atgggaaaac gatcgcatac gagaaagtaa tttcattata ttttaccaaa tccaggatga
     3661 agcagaccat aaattcaaga gtttcatcca tgacagtgag taatgaggta tacaatggtt
     3721 ttatacagtt tgatggagat aaaaaggtgc acctttttac caagggcaag aagcaaaaat
     3781 tagttgattg cttaatttcg atcagcaaat tcttaaaaac cgaggtaaag gatttatccg
     3841 gaagttaagg gatttactgg tttgccaaat agtaaaaata ctaggttcag tacataatca
     3901 gggattatca aaattgatta tcaggcaaag agcaaagaaa acccgggaac gacttcccgg
     3961 gggctcatca gcattcttat caaattttca ttcttcaaca tactcccggg cagactgtcg
     4021 cattaccctg atcttaccat tcaccagctt gtatgcctca tggagaatat agttctcttc
     4081 accgtccgga cccgatgtgg tttcatctat ccaaaccaga acccattcct cgtttaagtc
     4141 tacgcttctc agggccattg atgcatgtct gttcagagtt atttgcttat tttctacata
     4201 tctcataata tcggcaatca cggtgtctct aactgtattt ctgaagaacc cgtctgcaaa
     4261 gaaaagggtt acgctgtctg caaagtagtt tccaatttca ctaaagtcta gagtttcaaa
     4321 tgccttcaga aaatcatcca ccacaacttc attttccgaa ttccccattt caaaagaacc
     4381 agagtataaa tactcatcac tggtataatc aggtatctgg tcggcttcag atggaatttg
     4441 agcataagca tatatagatc ggattttccc ctcctcaatc cggtataatt catgtgctat
     4501 aactctgttt tctccctctt catttgtcat tgattcatcg gtgacggata gcacccaaga
     4561 ctcattgcga tccaggcttc ttactgaagc tccggcaata taagcgattt taatatcgga
     4621 catggtagct aacatttgat ctaccactac ccttagtgaa tctttcgtag tatcaaaccg
     4681 gtctccattt gctaaaataa catggattga atctgctaaa tatgagaaag cctcatcaat
     4741 attccctgct tcaagagcat cattccagtt tcgcacgagg gttagataag cagggtcgcc
     4801 catttcaaag gaattggaat agctgtattt cgcaactgtt gtggaatcag gagtttcggc
     4861 tgattcatcc gaagtagaag tgccacatgc actcattaaa aatgcaaaag cacaaaacaa
     4921 aattagtgat ctcatttaat taggtatttt tgtgaaatct caatttatgg gaaacccaat
     4981 taagagtcaa tataaaacta atcccggtta gtattcttat ctatttcaaa gtgatatatt
     5041 caattaaacc ttgaaagagt cctagttgga tggttgcaaa tattattgct aaaaagtgcc
     5101 tggagatcaa atttattata aaggggttag cttactatct attatccagc aagtatgaag
     5161 taaaactcct tccatctact ttcgtgtatt taaaatcttc ataaccttca aaagaaatat
     5221 tcttgaaatt aaccggagtc ctgaacttcg tatacttaaa gttcgccaac tctaaaaagg
     5281 tagcggattc gaaatttgca ccgtttggaa attcagcata tttaaaattc gcatcatccc
     5341 tgaactctgc gtcattaaaa aagggcaatt cagaaaattc ggcgtatttg aaatttgctt
     5401 cacgattgaa aaccgatccg ctgaagtcaa cattttcggc aaattcagaa tatttaaatt
     5461 cacttttccg tttaaaagta cagttcttaa aaaccacatc atcatcaaaa tgagctatga
     5521 atgtgtcatc gtttctttcc aaatggtaat aggcaataac atcgtccagg aaagtgcagt
     5581 ttatgaaagt tacggaggct tccacagtac tttcatacga atcattacta ttaccaaacc
     5641 aactaaatga agagtcttta tgccgacgat tacgaagatc tgtaaagtcg agattaccct
     5701 caatctcaac atctttaaaa gagactgatt tgccttcatc aatttctctc aaaatttccc
     5761 gtgcattaac tcgctgctgg gcaaatgctg ttactgtaga aagggtggcg aggatgatca
     5821 cataaatctt tcgcatgatt ttattttagt ttgattttct taatttctaa ccaataatgt
     5881 gccagtaagt caaaatcact cagaagaacg aaaaatgaac attgaggtta ggaggaagca
     5941 tttctgctgt ccgcttctga tcgttaccgg acgattttct ttgaagtcaa tctgaaaata
     6001 actaaactgg aagcctgtcg acatcatgcg aaataacata catctcgtca tccattactt
     6061 aatttttatt gcatgtatcc tggcaatcag tggctgcaag tattactacg gagcctttta
     6121 tacagatcca caaaaatgtt ttggcgatgc cttaagagaa aagccctatg atatagtaat
     6181 agtacctggt ttcccatcag attccggaaa aataaatact acattatctg aacggatttt
     6241 atgggcagat tatttatata aaaatggaat agcaaatcaa atactttttt caggatcagc
     6301 tgtttataca ccttttattg aaggtgagat tatgcgacta tttgcattgc aacttggcat
     6361 tgatgaaaat gaaattaaag ttgaaaccaa agcgcttcat tccactgaga atctttatta
     6421 tggattcaag atggccagtg agtctggtta cgaaaaaatt gcctttgcga cacatccggc
     6481 tcaatccagt ttcctaaaac aattcaggag aaaattcaat ttggatttag attttattcc
     6541 tattgttgcc gattcgatca aaaacatcaa atactctttc cagtccatag atctatcaaa
     6601 aatttttgtt gaagatttta ttccattaaa tgaacgacaa ggattattca aaaaacttcg
     6661 gggtacaaga ggcggtaggg taaaaaaaga aataaggaag gaaaagcgta gcaatgacta
     6721 acgacagatt ttcatgcgtt aaataatatg caatgaatag aattttgcca tttatcatgg
     6781 tcaaaaccca ccacccgaat ttattaaatt tgtccgcttt gctgtaacac atcctgtgtt
     6841 gttgttgtca aagtattatt cacacatatc ctgtatccat gataaagaat tatctgacta
     6901 tctcatttcg ccatctcctc aagaacaagg tttatgtaat aatcaacgcg gttggaaccg
     6961 gaattgctat tgcctgcggt cttacggctt atctactcat tgggtataat tacgagtttg
     7021 atgactactt caatggaaaa aaggctcaaa atatagtgaa ggtgatgcac cattttaaaa
     7081 catccggggg aaattcagat caggagttgg caattcctgt ggggcttagc gaaagggttt
     7141 ttcaggaagt ttccggaatt gaaaatgtca cacgattctg taatcaacat gggattctga
     7201 gttcggaaca cgattctttt tttgaaaata tcagatttgc agacgcttct ttttttgata
     7261 tgttccatcc tgaactgaaa tatggttcgt ataagaattt taaagcacgt gaaacagttt
     7321 acttgacaga gccaattgca aaaaaacatt ttggtgatat tgatccaaca ggcaaaccgg
     7381 taaccataga aattaatgaa cgcttatatg aggttttggt tggaggagtt tttgaaaaac
     7441 tgcccctgaa catcagcttt actcttgacg ttctgctcag aatcgaatta tattttgacg
     7501 caaattcaat ttcttcaaat tctcttgacc attacacatc tgctttgttt tcgttaaccg
     7561 atagtaaaaa caccaggttt gcaggcgagc aattgaatat atatttaagt gaaattaatg
     7621 ccagcgataa agaaagaatt agtaccagtt tcgaattgat accctttgaa agaaaggtaa
     7681 ttaacggaga ggtatctgcc agtgatttaa ggttacctat tcccacgatg gctttactgg
     7741 tatttggaat tttggcaacc ataattttgc taatcgcctg cttcaatttt acaaatacta
     7801 ctcttgcact tacaaatacc aggtttaagg aaattggagt aagaaaagtt gtcggagcac
     7861 gaaaaagtgg aattgtcatt caatctctca tagaaacaac gttaaccatc cttgttgcaa
     7921 ttagtttagg ggtattaatg gctcaaatta ttgttcccag gtttgctacc atgtggcgat
     7981 tggaatttgg gctgggcgat ctaagtacta ctaattttat tatgataatc ggattattgg
     8041 taatggcgat tgtcactttg gccggaattt atcccgcatt tcaaaacagt aatcttaagc
     8101 caatcgaatt gctgaaagga aaaagaaaaa tcaggggaac caatattttt agccaaacct
     8161 tccttgttct gcagttttcc ctttccgtga taatgctaat tgcaggagtt gtgttcaccc
     8221 aaaacgcgca atatcagaag gagcttgatt ttggctatga ttataaagat gtgatagcaa
     8281 ttactcttta cggtgaagat gattttaccc gattcaaaaa tcagatttca ggattttcac
     8341 agattaaaga aattgcagga gctaaaaacc caatcggacc ctacaccgcg ttaaagggta
     8401 gatttactat tgataccact caattcacct ccgatctcca ttttattggt gataactact
     8461 ttaatgcaat tgggttaaat cttagccgcg gcaggaattt taatgctgaa atgatctcag
     8521 atcgtgaatc ttccttaatt gttgacgaaa atttcgtttc aaatcataac cttgccaacc
     8581 cggttgatgc cagggttatt tttgatgaaa aaccttaccg gataattggt gtagttgaaa
     8641 atcacctcag tggattaaaa aaagagcgaa aaacggaaca tgtttttgca ttggcagtga
     8701 ataccgactt caaatcaatg ttaattaaga ctattggagg cgaaaatctc gccatattta
     8761 catacctgga gaaggagtgg agaaaaatta accccgcgaa acccttcctt ggtattatgc
     8821 aggaagagat tttgtttgaa gaggccaatg ggtacaataa taacctcaca caaatattct
     8881 tttttctaac cacattaggg tccttgctct ctgcatttgg gatctatgcc ctggccaagc
     8941 ttaatgtaca actgcgattc cgggaaatcg ggattcgaaa aaccctgggt tcaggagtaa
     9001 gtaatatcat aaaactacta aacagggaat tcgcattaat tttaaccatc gcggcaattg
     9061 ccggggggtt cattggatat tttatgacca atgctttact tgatgactta tatacttatc
     9121 acctggagat aagtattttt acactccttg tctcaggagt aagtattttc atcattggaa
     9181 ttatttctac cagtagcaca atcttccggg ctgctaatac aaatcccata gagatactaa
     9241 aggatgagtg attctgacaa atttcctgag attcaggtta taaatcagat tatatgttct
     9301 caacttcctg tttcagagtc taattctgat ttaaaattca tatcatttga ttttgatcct
     9361 gccaatccaa tttcctctga aatagatttt catgcaatcc aggcacagcg tagagtttat
     9421 catattcatg aaatccaatt ttctccaccc ttctcgagta ttaagaagaa agaattcttc
     9481 gtccattttt gtaatcaatc ataataggtg aaaatgcttt tggttcactt ctatataaaa
     9541 aaaaattcga caaaggcaaa ttaagtttct aattttattg atattggaag cctttgttgc
     9601 attatatatg gttcttgtaa atgaaatcat ttagcaaata ttttgggagc atgaattcaa
     9661 tcttcaatca tatataacca gtatttaaaa taaaataatg tccattgtat ggatgacctt
     9721 cccgtaaaag ttgctttacc gttattccgc aaagagttca atttagatat tcctggtgga
     9781 gatgatgaga ttgtcaggat tgttatcggc agaatcgttc tttatattcc aaatttcgag
     9841 acacgcaaaa aaattgtcct gcttcatgac atccatcatc tggcaaccgg ttactcagca
     9901 ttcttcaaag gtgaaactga aatcagtgca tgggagttat ctgcaggatg ttacaataac
     9961 tgggtagcct tcataattaa tatttacgct ttcatgatcg gattaattat aaatccgact
    10021 aaggtttggc gtgcctgggt aagaggtaga aatactcgta ccttgtactt catgggttac
    10081 cattacgatg aacttcttca aaaaacagtt ggtgcaatta aaaaagagct gggattaatg
    10141 agtgggatta atcaccgaag attactgaaa acaattgttt cattcatcct tatgataatt
    10201 attggcttag ctgtttcaat tttatctatt ttactcgttc ccctcacaat tttcttcacc
    10261 ctatatatcc ggctgaaatc aggatgaaaa aactaaagac agatgtcctg attgttggcg
    10321 cgggccctac cggactcatg gctgcgaatc agttgcagcg atttggtatt gattttatga
    10381 tcattgacaa gaaatcaggt cctactatgg aatcccgggc aatagcggta actgcgagaa
    10441 gccttgaaat ttatgaacaa atgggcttgt cagatacggc aattcaggaa ggacaaagaa
    10501 ttgaaatttt taatttatat gtaaaaggca aaccaagagt cagcgttaag ataggtgaaa
    10561 ttggcaaagg actttccgat ttcagttata tgcttgcctt cgagcaaagt aaaaatgagg
    10621 tgttgctttc ccgcaatttg tataaccacg gcaaatcaat tcattggaat tatgaatttg
    10681 ttgaaataaa agagaatcag gataacgtaa cagtactcgc cagaaatcca gacattgagg
    10741 tggagataac cgctttgtac cttattgcat gcgacggagc cggaagtcca atcagacaac
    10801 acctcaattt tagtttcaga ggcggaacct ataagcataa attttttgtg gcggatgcca
    10861 ttctgaagtg gtccattccc tatgatcaac taatcatttc ccccggaaca gaaaatttct
    10921 gcgcctttct tccgctccag gggaaccata cttacaggat tatcggcaca cttcctaaat
    10981 cttatttcaa tacggaaaca gttacttttc atgatatacg ggaagttgtg ataagaacac
    11041 tcggtattga aatagaattc gagcaagtga actggttctc aacatataaa cttcaccatc
    11101 gtggggttaa caagtttgat aataaccggg tttttttggc cggtgatgct gcccacattc
    11161 atagtcctgc cggtggccag gggatgaata cgggattaca ggatgcctat aacctgtgtt
    11221 ggaaactggc tttggtatta aaaggccagg catttgaatc tttgctgaag acttataatg
    11281 aagagcggct tccttttgca aaatggttgc tcagatttac cgaccggggt ttcaacatga
    11341 tgaccaacag aaattttttc atccggaaat tcagggaact tattgcaatt aatcttgtca
    11401 aatgggtgcc aagggtgggt tttataaggc cggtaatttt caaagtgctt tcacaaatct
    11461 ggtattcgta tcatggtttc agcctggcgc aatcgtcaac ccgccagaat ctaaagtttg
    11521 tttcaggtga tcgacttcct tatttcccgg gcgaaaaatt ctattccttg ttcaaaaatc
    11581 cttcctttca cttgttgtgt ctgacaaata cttcgatttc tgacattcaa aaacaaaatc
    11641 ttcaagactc ctttcctttc catataaaaa ttatagaaca gaaaataaat gagatgtgga
    11701 ataaattagg cgtagttagt gaattgtata ttgtagtcag gcctgacaat tacatactat
    11761 ttatagcgga ttccatacac aaaaacattc ttgagcatta cttcaaagat tttccaaaat
    11821 gctgattctg atacccgcat gtaacctgtc agaataatct tctgtctaag aaacatggtt
    11881 tgcacatttt gatgaaaagc aagcagctgc attatctatc tttttaatta ttggacttac
    11941 cctcctcgta ataaaactta acagtccttt tggttggatc ttctggctgg gtctatatct
    12001 cgttgccaca ggtttcattg ggttatcttt ttgtcttgga ttttttcttc tgagcaaaag
    12061 agctggatgg aaattcttga ttctctgaat gctgagaatt aatttcccgc taagttattt
    12121 tcccatgaat atttgattcc ctgtttattc ttaattgtca aaatctgatt atctcaaaga
    12181 ttgttattgt tttattttaa tgctctaaag cgactgaaat tcgttacttt tactttgtga
    12241 ggagcattca aattacaata aaagatttag caaaggaact tggaatctcc ccttcaacag
    12301 tttccagggc attgaaagat catccggata tcagcaaaga aaccaaaaga caggtaaatg
    12361 aacttgcaga aagactgaat taccaaccta ataacattgc actgagtctc cggcaaagga
    12421 aaaccaacac aataggagta gttatacccg agataattca ttttttcttt agtaccgtta
    12481 tcagtgggat tgaagatata gcttacaaag caggttacag cgtaattatt gcacagtcaa
    12541 atgaatctta tgacagggag cttaaagaca ctaaagcttt atttaacggg aaagttgatg
    12601 gtatgctgat ttccatttcc cgtgaaacca gtgacttcgg tcattttgaa agtttgatgg
    12661 aaagaggcct gccactcgta ttctttgaca gggtatgcga tgctataatt accgacaaga
    12721 taataatcga tgataaggag gccgcagatc aagctactca gcacttaatt gatcaagggt
    12781 gccgtcggat tttacatctt tctggcccta aaagtttact gataggtcaa caaaggcttg
    12841 gagggtacct gcaagctttg gagagaaata aaattcccgt gtctgatgaa ctgattatcg
    12901 actgcggaca tggaactgaa atagaagctg ctcagaaagt tcacgaattt cttcagaaat
    12961 accctgcatt cgacggtgtt tttgctacca atgatgttgc agcaatgggt gctatgatgg
    13021 cactaaagga aaaaaacgta aaaatacccg aagaggtagc agtcattggt tttggaaact
    13081 ggagatttgg cgcattaacc gatccgccac tttctaccgt agaacaaccc ggctacgaaa
    13141 tgggttgcga agcagcgaaa catttgattc gccagataga actgaaggat gacgaatcct
    13201 ttgaagcgac tacgaaaatt ctgaaaacca gggtaatccc cagaaaatca tcgctacgaa
    13261 attcctaaaa cgtaaccgca atcgtttgca agaaattttt gacgttaata ttcacttcta
    13321 aagattgaat ataattacct taagagctta aaaatttacc tcctttgcaa aaaacgatca
    13381 ccaggaataa gactttgaat gatggtgacc actacaggtc tctgggaact cttcaaagtt
    13441 acagcaaaac caatttaggg attgaaggct caacctcaca tggatccttt ataatcactt
    13501 tttacaatga atcaatagta aggatacggg tttgtgaaga agattttgaa gaagacttca
    13561 gctattctgt catagcaatt cctgaaaatc cggaatttat tgtcgaagaa ataaatggag
    13621 aaattattct ctccacaggc aaactagtgg ttaagatcat gaaagatcca cttcgaattt
    13681 cattcctcaa cctgcagggc agcgtaattt gtgcagatga caaggcattc ggaacttcat
    13741 ggctgggtac gcaggttaca acctacaagg aattacagaa aggggaaaga ttcataggcc
    13801 tcggggagaa gaccggacct ctggacaaac agggaaaggg ttacgaaaat tggaatactg
    13861 atgcatttgg atacggagta gataccgatc ctatttattc ctccatccct ttctacatag
    13921 gaattcacca caatctgcaa tacggaatat tttttgataa cagcttcaaa tcacatttta
    13981 atttcggtgc ttccaataat agattttcaa gtttctccgc agatggtgga gaaatgaatt
    14041 attatttcat ttacggggaa acaataacag acgtcatttt tcaatacaca aagctgaccg
    14101 gaagaattga attaccgcct atatggtcat tgggttacca gcaatgcagg tacagttatt
    14161 cttccgaaga agaagttttg agaattgccg atacttttcg catgaaggat atcccggcag
    14221 atgctattgt tttggatatc caccatatgg ataagtataa aattttcacc tggaacccgg
    14281 caactttcaa aaatcctgaa aaattaattg caaaacttaa acagaaaggt tttcacgcgg
    14341 tagttatttg cgacccggga attaaaattt ctgctgacta ccacgcttac aaaaaaggaa
    14401 ttgaaaatga tatattcctg aaatatcctg atggtgcact atacgaagca caagtttggc
    14461 ctggttggtg tcattttcca gacttttcga aagcagaatg ccgcgaactc tggtcgcagc
    14521 ttctgcaaca ctatacacaa gcgggtgtaa caggtttttg gaatgatatg aatgaattgg
    14581 caacctgggg acaaatgatg cctgaaatga tccaatttga ttatgaaggc aagaaggctt
    14641 cactccggcg aatgaaaaat ctatacggtt tgttaatggc aggagccaca caaacagcag
    14701 ccagaaaaca actcaatggg aagcgaccct ttaatctgag ccgctcaggg tttgccggga
    14761 tccaacgctt tgcggcactt tggaccggcg ataacacttc ctcagaagaa cacatgctgc
    14821 tgggagttcg catggttaat aaccttggaa tttccggtgt tcctttcaca ggatatgatg
    14881 taggggggtt taccagtgat gcaagtcctg aattatttgc ccgctggatt tctgttggcg
    14941 ctttttctcc attcttccga ggacattccg tcattaatag tcgcagcgcc gaaccctgga
    15001 cattcggtga gcgtgtttta gaaattagca gaaattatat aaagctcagg tataaactct
    15061 taccgtacat ttactcttcg ttttacgagg cttcaattaa cggcctcccg gtttgccgat
    15121 cactggtaat taattatccc catgaggcca aaatttttca tcaagatttt caaaatcaat
    15181 atttgtttgg tcagaacata atggttgccc ctgctcccgg caatcaggag ttatcccggg
    15241 ttttccttcc agaaggccta tggtatgatc tgtttacaga taaagtattt aatggcactg
    15301 aagagattat tgtagagtct cctttggaaa agttgccgct atttgtaaaa ggttcaggga
    15361 ttctgtttct tcaatctgaa attgcctcca ccacccagaa accggatgac accctcgagg
    15421 ttcacattta taacggcacc ttagaatcct attttactta ttacgaagat gatggagact
    15481 cataccaata cctcgagaat gaattcttca ggcgaatgat tcgattcaat ccggaaaaca
    15541 gatcaatttg ttttgaaaaa tccgaaggcg aacttaattc gaaatttaaa acactcaggg
    15601 tatttcttca tggaatgaaa aatattgata aaataaatgt agatggcaaa attctggagt
    15661 tacaagtcag ggattttcaa tttataagac ccatctcatg ttttgatccg attcattccg
    15721 gaaaggatta tgaaatcacc ataaacaatt taccctattt tgaccttcct aataatggtg
    15781 gcaaaaccga aattcattat tagataaatt ttaatttatg aaaagcataa aacaaatagt
    15841 atttcccgca ttcatagtat ttcttgcatt tagcttttcc tgcactgcac caaccaagga
    15901 gaaagaacct gtagaaatta cctccactta tcctgataag attaagaatc tcaccatcta
    15961 tgaagttaat gtccggcaaa tgacgcccga aggaaccttt aaggcatttg aaactcatct
    16021 ccccaggcta aaaaaaatgg gcgtcggaat tttatggttt atgcccattc atcccatcgg
    16081 cattgaaaac aggaaaggca gtttaggaag ttattattca gtaagagact atcgcggagt
    16141 taatccggaa ttcggaacca ttgaagattt taagaacctg cttgagaaaa gccatgagat
    16201 gggattcgaa gttatcattg actgggtggg aaatcacacc tcctgggatc atgcgtggat
    16261 ggcagaaaaa ccagacttct atacccgcga ttcaacaggt aatgtaattc ctcctgttgc
    16321 ggattggtca gacgtggcag accttaatta tgataataaa gaattatggg attatatgat
    16381 ctctgatatg aaatactgga ccaatgaaat aggtattgat ggattccgct gcgatgccgc
    16441 accctggctg ccgggagaat tctgggtgaa ggcaattcag gaattagaag ctgaaaaaga
    16501 tttattgatg cttgcagaag gtgaaggacc cgaatggcat gaggctggat ttgatatgac
    16561 ttatgcctgg gaatatcatc atattatgaa tggaattgta agaggagaaa agaatgttgt
    16621 tgacctcgcc gaatatatgg aaaaagaatt gagctcttat cccaatggtg gctacagatt
    16681 aatctttact tccaatcatg atgaaaattc ctgggcaggc acggtaaaag aaagatttgg
    16741 cgatgcggta aaaacttttg ccgcactttc atttacctat tatggctatc ctctgattta
    16801 tagtggacag gaagcaggaa tggataaaag acttcgtttt tttgacaaag acaccatcag
    16861 ctgggaggat ctgagtatgc aaagtttcta ccatgaactc atacaacttc ggaaagatga
    16921 tccggcgatc tggaatggag atgaaggtgg caatcctgag atcataaata ttgataatct
    16981 ggaaaacgtg ctggcattct ccagaatatc cgggaccaac tcagtcattg gttttttcaa
    17041 tctttcagat actgaacaaa gctttagtgt aaaggaatcg gtaattggag attatgttga
    17101 tttgatttcc gataatcagt attatattaa cggacaggcg ccgataacac ttcccccctg
    17161 gggatttatg attctttcaa actagatcat ggaagagaaa cttcatgctg aacaagaatt
    17221 atctttatca tctgaaaaac ctgcgctaag cttctggcaa atctggaaca tgagtttcgg
    17281 attcctggga attcaattcg ggttcgcgct tcaaaatgcc aatgttagca gaatctttga
    17341 aacgctgggt gcttcccagg acgaactccc aattttatgg ctcgctgcac ctgtaaccgg
    17401 gttactggtt cagcccatca ttggatactt cagcgatcgt acctggcatc cgcggtgggg
    17461 aagacgaaga cctttctttg cggtaggtgc gatattggca tcattggcgc tctgcataat
    17521 gcctaatgca gggttgttat ggattgcggc cggaactctt tggctcatgg atgcatctat
    17581 caatattagc atggagccct ttcgcgcctt tgtgggggac atgctgcctg gtcatcaacg
    17641 cacaagaggt ttcgcgatgc aaagcttttt tataggaatc ggagccgtgg ttgcttctat
    17701 gcttccctat attctcacca attggcttgg cgtttccaac accgcacctg ccggtgaaat
    17761 acctaactcg gttaaatggt ctttctacat gggagcaatt gcattcttct ccgctgttat
    17821 gtggacggtt agccgaacaa aagaatatcc tccggatgat cttgaaaaat tgaaagctga
    17881 caataaaaac ctcagttcgg ttcagatact tagggaatca ttcgaaggga tttttcagat
    17941 gcccaagaca atggcacaac tcgctgtcgt acaatttttc tcctggtttg cccttttttc
    18001 tatgtggatt tatacgacct cagccgttac caaacatatt tacggaactt cagatacaag
    18061 ttccgcttta tacaatgagg gagccgactg ggtgggaatc atgtttgcta cctacaatgg
    18121 cgttgcggca ctgacggccc tcatgctacc ctggctggca aaagtaactt caaggaaaat
    18181 cacacattta atctgcttat gttgcggagg cctgggtctt ctctcctttt atatgattaa
    18241 agatcccaat ttactgatta tgtctatggt gggagttggc attgcctggg caagtattct
    18301 ctcggtgccc tatgctatgc tttccagttc actccccgct aaaaagatgg gatactatat
    18361 gggcgttttc aattttttta tcgtaatacc ccagattgtt gcggctggaa tcctgggatt
    18421 tatgcttcgg aattttctgg acggagcagc aatcaatgca ataattgtgg gtggtgcttc
    18481 aatgattttg gcaggtttcc tgagcctgat tgttgatgat aaggaagaag tacatctcag
    18541 tgaagagatt cccggaactc acaaaccgga atcttgattt gagtgaagaa ttcattcagg
    18601 ccctacgctc cggtttggtg tactacgtgc cagccaaatc cgggccagtg tgatatgcaa
    18661 atttaaagca acacagcctg agaaaagttc aataccgggc tggccatcgt tatatcggca
    18721 ttaccgaatt atacttacag aatgaaatag aaagattaca agaaggaaat taaccggtgt
    18781 catccgttag gatagtggtg gcaaattgcc ttcaagatga aaaaagagag ctatgaaatc
    18841 attacctttg agaaagatca gaattatgtc agagatcata ttcattgtgg aagagtcagc
    18901 tgaaggagga tttgaagcaa aagcattagg tgaatcgata tttaccgaag cagaaagcat
    18961 ggaggaattg aaggagaata tccgggaagc gatccggtgc cattatgatg aaaatgctcc
    19021 caaagtagtg cgtttgcatt atgtgaaaga agaagtcttt gctgcatgaa attgcctcgc
    19081 aatgtatcag gtcaagaact gattaagaag ctaaagaaac taggttacct gcatcagtcg
    19141 tgatcgggct cccaaactat ggcctcgttt gtaacgaggt gcccagcgag tagtgagatt
    19201 gtatcgcaca cataaaaatg aaaaatactt caacgctatt gaagccaaat aaaataaaaa
    19261 aaactaaatg aatttagaat tcaataataa ttttactgcc agtcgtctgg ctttaaccgc
    19321 attgatgcat gaacgaggct ggatatgcat gggacttcca tcctcgtaaa aacctcaaac
    19381 cattccccaa tctctatacc tattttcagg tgatgttttt ttatgaattt aatagccatc
    19441 tttacctgac aagtttttgg gatatttttg gcttccagga acttgaagag taagattctt
    19501 cctgctgttt gctgctttcg taagacccga tttgagctcg cttcatactt taaatcagag
    19561 tgttctggac aatcccacaa gtgaaagtca aattttaact gaacttgttg ctttgcgcaa
    19621 caatttagct ccggctaaag cattaatagc tacctactct tttgatccat tgattggcat
    19681 gacatcacaa accgatacga acagtcaaac taagtggttt tactatgact ccaatggccg
    19741 gttatcttta atcaaggatc aaaatggcga tatcgtaacc cagcatgaat acaactttgt
    19801 agtaaaataa aaattctgcg caacattttt caaactaaaa tcaaaacaga tatgagagaa
    19861 taattaacca atcaaaaact tcgtagaatt cctgggaaaa aattttgtga aacgtctaaa
    19921 ccaatttaac aatgtgtaaa ttaatttttt ccagagcttt ttttataatt ccgtttttac
    19981 ttttagcggt tgcttcctct tgccgcaatg aaaccatgga tgataccgac cctacggttt
    20041 ctataacaaa cctgatggag aatcagaagg taaggggaaa aattaccgtg aatttctctc
    20101 caatggatga gcatctcgat aaagtttatt tctttattga taatgtgtca ttgaaaacgt
    20161 taacccattc tccgtggata atggatattg ataccgaaga tttcgaagat ggtaatcatg
    20221 caataaaagt aatagcagcc gacgctaata gtaactcttc aacggccgaa gttaatgtag
    20281 aattcaaaaa cctgatgctg actgttgaaa ttggcaattc ctttgaagaa ggtcaaaaca
    20341 gttatataat aatttatgat atggaaggtg atgccgtaga ttatcgcctg atagaaaata
    20401 attcaacatt taaatttcca tacccggatg attatgaagg atgggatttt aatgcaactt
    20461 attttacagt taacaacgac aatttcgggc aaagttttta ttcagaaacc attcagcgtt
    20521 gcagtgcgcc tcgctgggtt tttcctgatc ttaccgatga tgaagattat tacgaacaaa
    20581 atatatcgct cataaatgtt cctgaccacg acgaaattta tgcaagcaac gggtttcaat
    20641 acaatggaat cggttcaaat aatactacaa aactttatac gctggtcgag cctgataatt
    20701 tattcgttgg tttaatagag ggccaggcag ccaggtataa atggattccc caagtagccg
    20761 ccggcagcat agaaattgat ttaactgcaa tgcaggaaat gctgaccggg agtgtgagcg
    20821 cccaggtgcc tgtttcaagt ttatatgtta aggggaatat aaataaccca ggaggtttgc
    20881 tctcttattt cctttccagt tcaaccaatg aacacttttt ttatgcagat gtatttgata
    20941 cctactcagt ttccttacgc tctgtcatgc caaatcttaa aactctatac caggtaatta
    21001 cggatgattt acctatctct tttcaggctc cgtcatttga tgccaaccta acctccgcca
    21061 accaggaaat ctttaacttt aatactgccg gagattttga ttatttttca gtgtattggt
    21121 atcattatga ttatgatcct atgacaaatt accaaagcgc acatttcgaa acattcggag
    21181 gcccggggga tgaggccaaa tattttcctc tgcctgaagt tttaaaaaat gtatatccgg
    21241 atttcgatcc ttcattactt gtgcccggtg gaatttcatt tcaagaaaat aatgtattat
    21301 caggatatgc gcactttagt ttcttgtata atactgatca gaattacccg gtgccgacac
    21361 gtattgatgc ctactcaatt gaacccgatc ccaacggcgg gcgcgttagt aaaccgggat
    21421 atgtaaaagt taagcgatgg ggcaaatctc gaaggtttaa ccagacattt taaatcgggc
    21481 taatcaaaaa acctgcgagg tttgcttaat ttaaactctg catatttcct acatatacca
    21541 aaacctgaga gaagttcagt acccggccgg ccatcgttat atcggcatta ccgaattata
    21601 cttactggat aaaatggaag gattacaaga ggaaattaac cggtatcatc cgctaggaaa
    21661 actcaaacaa ttaattaaat tgttaacttt aaacagagaa ataatgaata gtatgaaagg
    21721 aataacattc atcactgacg agacacacaa aaagcgcttt gttcaaattg acttggagca
    21781 gcttgagaaa tatcagcacg aaatagagga tttactggat gtaatcatag cggagagcag
    21841 gaaaaatgat gaacaagtaa gttgggaaga gattaaagca caattgaaag ctgaagggaa
    21901 gctgtagtgt atcaaatcac cttcaggaaa aaagcgggca aagagctttt gaggctatcc
    21961 agtcaaatag tgaagaaagc ggccaaatcc atagacagtc tgtccaaaaa tccccgcccc
    22021 aaaggaagta aaaaacttaa agctagtgat gaaaatatgt ggcggatcag gatcggagat
    22081 tatagagtct tgtatacgat agatgatcag gtgaagatta ttgatattag gaaagtgggg
    22141 catagaaaag acagagggtg ttcaacgaac tgtgtcaaga taaaaaatta ttaacttgat
    22201 gcaaattatg aaagaaaaca tgaacatgga agagtttcgc aaacaagcag tggaacttct
    22261 tcgacaagga aaaccgttaa cgggaaaaga tggggtaatg acgcccctga taaagcaggt
    22321 tctggaagct tcgttggagg gtga
//
LOCUS       DASWHX010000066        12764 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_3010576, whole genome shotgun sequence.
ACCESSION   DASWHX010000066 DASWHX010000000
VERSION     DASWHX010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 12764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12764
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3010576"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(538..1785)
                     /locus_tag="VGA21_04550"
     CDS             complement(538..1785)
                     /locus_tag="VGA21_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009581580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEX9649805.1"
                     /translation="MTDFSPQTLLLLILIIITISFVFGKILDFLNIKVQKKKVPEPMK
                     DYYDQEKYEKSQEYLKVNTRFSFWSSFFSFILSFTVIAIGIFGWLDGHLRNYIENPLL
                     LSLAFFGLIFIVSDILGTPFQLYGTFVIEEKFGFNKVTPSLFIKDKLKGYLLGILLGG
                     PLLALLIYLIQTFGPDFWIYFWAAVSLFTLFMNFFYTTLILPLFNKLMPLEEGELRQA
                     IQDYSNKVGFPLKNIFVMDGSKRSKKANAFFSGFGRKKKVVLFDTLIENHSVHELVAV
                     FAHEVGHYKKKHITWGYLTSTIQVGITLFIMSYLIFNANLSIALGGNQLAIHLNLIAF
                     AILFSPISGITGIFMNMLSRKNEFEADNYAATTFEGEFLKTALKKLSADNLSNLEPHP
                     AYVFFHYSHPPLLKRLEAIKSQE"
     gene            complement(1806..2879)
                     /locus_tag="VGA21_04555"
     CDS             complement(1806..2879)
                     /locus_tag="VGA21_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009579033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HEX9649806.1"
                     /translation="MHKQKFSRFASINSIITLIGVMSVILFSSKPVKAQDPQFSQFYS
                     APLYLNPAFVGSTNWTRVGANFRTQWPAIDANFVTLSAYADHYLDDQNSGIGIIITGD
                     REGLAGLRSNSIGLQYSYQLPISKIFTVRAGVHVAYVFRDVNFGKLTFGDQYDQNGFI
                     SVPTAETFNTGARKSFLDLGFGGLLYSENIWLGVSAFHMNRPNQSLIGESSKLPVKYS
                     VHAGYKFFFKPGVMGQGIYATPQERSLAPTIQYRKQGIFSQLDIGLYFTYEPVLFGLW
                     YRGVPYKQFDGFTNNEALVFMVGFEKKAPKDALYIGYSYDFTISQLGASAGGAHEIAI
                     SYSFFTGDPRKPPKNVRQIPCRR"
     gene            3052..8241
                     /locus_tag="VGA21_04560"
     CDS             3052..8241
                     /locus_tag="VGA21_04560"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HEX9649807.1"
                     /translation="MPERIVKSVVLICLVFICCSAVGQARSYSDLNWAFGNSNQWISF
                     NKSNSNPVLLSGKGSQGVGGAATASHPVTGDLLFYTDGVTVFDASHQVMTGGLGLAGN
                     SSANQPVAICAVPGLPNQYYVFSNSADYITPGTIFYTVVDMNQFGNAVFPMPALGEVN
                     ASIKAKATSLNNTAEAMIIVSKSDLSGFWLISKRSGNNDAYDVLDISNGLENITGAGS
                     FNLGDPTIGGNISYNEATGRVAVSSQIENRNIQILQFDNATGILAFDSEVLNTGYNNL
                     SGEAIYDTEWSGNGNYLYISIHGGGGFNGDLLQYNYNNPLNSLTSILPSVITNSYGLK
                     MAPDGNIYHLFQSGAEVRLARINEPDSIAAQTNYELNPLGSINFNGLQFPEFLPEYDL
                     MITADFTFEGLCANVPTHFFPEVTPVADSILWDFGDGTGANDYSPIHTFDGQATPYTV
                     GMTAFVSGQSMNITKQVNILAFDVQITLVADTVACKEEFPPPRGSSTPTQFSVTASFP
                     NAQPTSIVWSNGDTGATLTPDSAGFYYVVATLGTCSISASVNVQEYGLMTNTAAIWYF
                     GQEAGIDFNDIPPTALSDGVMTASEGCTAVSDQNGQILFYTDGSSVFDQDHNEIATNI
                     GGDPDATQSVMAVPFPSDQTLYYIFTAEAVYGTNTFRVKYSIFDLKLNNGTGDIIKDN
                     VVLFEKSTERLTSNGGWLILHEFGNNTFRAYPILASGIGTPVLSSIGSVHSASIERNG
                     HGYMKLSQDNSKLAIALSNNDNSNFVEMFNFDPSTGELSNYTQLDLGAQGAIGQVYGI
                     EFSPGGNKIFATVKNSGSSLIYEYRADTLMLASFIGTLPATPFAGEFGSIQYAPDGQL
                     YVAINGSQNLGVISVQEDVTQLSTFNAIGFTLAAGKQSLLGLPNFVQNFATQTGVPGM
                     SLPSLACVNNPVDLVGNASSIIDEFEWTILSPSGGPVFSSQNQTDTFTPTVAGTYTVN
                     LRVFNRCGLDQSFSQPLDVIDNPPPPDLLSLEIICDDNGVTLQALIVDDPALTYAWST
                     GETSKDITVLQQGNFTVTITNPQGCTSSATVLVLDARPQVELGPALTVCQGDFVTPLN
                     AGVTGATYAWTLNGGSPSSAQTRAVDTSVPGVFKFVLFVQDPAPFSCSTTDSVTVTVL
                     PDPVVNVTVTPSPGCNMNQGQIDINITSTGFYSYTVSGPTPSFASNFNGPTNFNLTNL
                     AVGFYNIVVTDEISGCSYIEVTSIVDAATPFAIQSATSGVGCGMVPVTVNLSGTPAFP
                     VTYSLTNQATGVITNGNGNTDPFTTSNVDPGNYTLLVTDAGICTQSFGPFNVTEGPKV
                     TAELVLDNCTNPATLTVITNSSNPVYSWTGPILTPSPTSNKATVEIPNLTATYSVTVA
                     DLGPLCDSTVTITVTPIPFQSASITSGGTSCDGQQTLTANVTPPGNYSFLWIRDGNNL
                     GGQVGQFMIATVSGEYTVRVRNQQSGCDLVTNPLTVEIFEPITVSINSGPLCDNGEPF
                     TLKADAITTADTVIYEWSRNGSVIAGQVADSLQSTTNGIYEVTATTEDGCSVSSSYTV
                     TLSPVNVITLPEASTICPLDSDPLLNSVELDAGTGFTQYMWFDNNGNIVSNNQVYTAS
                     IEGKYTVIGTNQFGCTTEASSVVIEDCVPRVFGPNAFRPKGTNDEFFLFTKYIIDFEI
                     FIYNRWGGLVYHSNQEDFRWDGIFEGRLLPAGTYAYKVRFKSEFNPLRGMMEKHGGVT
                     LLR"
     gene            8260..8616
                     /locus_tag="VGA21_04565"
     CDS             8260..8616
                     /locus_tag="VGA21_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009196995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEX9649808.1"
                     /translation="MKKISYILLILIFSAPIINAQAQVLKTNLTITVLDDNGNIQEGA
                     TVILYKNETDYRESKNPAIEKQVTDKKGRVKFKDLDTMAYFVEVRKGDLNNDGLGAQT
                     GELVAGRTNKANIIIQ"
     gene            8636..9040
                     /locus_tag="VGA21_04570"
     CDS             8636..9040
                     /locus_tag="VGA21_04570"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEX9649809.1"
                     /translation="MRIPIQVRYSDFDMMGHLNNSAYATYFELARLQFLMSLFEQNSF
                     KWTDVVANLNIDYRIPVVPQSKPIMYISCSRIGNSSYDLEYKVVEETDETHLYAEGKT
                     VQVCVDPKTQTSQKLPDPAKEILSNVLVPKKE"
     gene            complement(9332..9493)
                     /locus_tag="VGA21_04575"
     CDS             complement(9332..9493)
                     /locus_tag="VGA21_04575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649810.1"
                     /translation="MSNKTRSILRLISILMILIPVLDKLDIMVIPALSDYVFWMVVAA
                     SGILLLTSK"
     gene            9572..10510
                     /locus_tag="VGA21_04580"
     CDS             9572..10510
                     /locus_tag="VGA21_04580"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008624226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEX9649811.1"
                     /translation="MEKAVTILKNGGLVAIPTETVYGLAGNALDERVVSRIFSAKKRP
                     QFDPLIVHCSSIGQISHYATLENPQARLLAEKFWPGPMTLVLPKKDVVPYLVTSGMDT
                     VAMRIPAHEMTLHLLRQLDFPLVAPSANPFGYVSPTTPDHVNDNLGDVVDYILDGGPC
                     QLGIESTIILFDNKIPTVLRSGSLSVEKIQTIIGEINVVTHSSSNPSAPGMLKSHYAP
                     GKKLVLGNIELLLGMPDYDRVGVLSFREKFEHPKIVSQIILSENGDLEEAATNLFSGL
                     RSLDKMDINFILAETVPNHGLGRAINDRLKRGASDK"
     gene            10510..11694
                     /locus_tag="VGA21_04585"
     CDS             10510..11694
                     /locus_tag="VGA21_04585"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEX9649812.1"
                     /translation="MKTVDSINFKNKKALIRVDFNVPLNNELQITDDTRIKSAIPTIN
                     KILSDGGSAILMSHLGRPKDGPNPKYSLKSLLIDLQSRFSGAVYFSSDCIGEEPQKLA
                     RNLKPGELLLLENLRFHPEETKGELSFARQLAALGDVYVNDAFGTAHRAHASTTVVAQ
                     FFQDKVCGYVMAAEIENADRVLHHSEKPFTAVMGGAKISDKILVIEKLLDKVNNLLIG
                     GGMAYTFFKALGGNIGNSLLEPDKLDLAKELIGRAKAKNVNLELPIDSIIANKFENNA
                     EHKVAKNISIPDGWIGMDIGPESRKVFSNIIINSRTILWNGPMGVFEMHNFTAGTIEI
                     AEAVVSATEKGAYSLIGGGDSASAINNLGFGDKVSYVSTGGGALLEYMEGKLLPGILA
                     LE"
     gene            11890..12606
                     /locus_tag="VGA21_04590"
     CDS             11890..12606
                     /locus_tag="VGA21_04590"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX9649813.1"
                     /translation="MKVRNWFALLLFIFPQDTAGQVWKLFETEQIHQKRAESGKNYFP
                     FLNESSFAIGLYELKSKVIDNQPVHHEDEVYFIVSGKAKLVAGDSTFDVSAGSIAFVR
                     AGVSHRFVEIEEDFSALVIFTKTPTKTGDADALAFELDKLRQPRNPEENVWNQFLFVS
                     TIRMGLYLLPQSLGGDQPMTHNVDEINIVTNGDATLVVGEEKIPVKMGSIVWVKEGNP
                     HSFTNLSEDFDVLIAFHIKP"
BASE COUNT         3830 a         2608 c         2623 g         3703 t
ORIGIN      
        1 gaatacttgg tgactttgtg ccttcgtggt taattaaatc cgtgccacaa gggcactaag
       61 acactacttt tttgctttca acttacttca gaatactttg gtgactttgt gccttcgtgg
      121 ttaattaaat ccgtgccaca agggcactaa gacacttagt tttttgcttt caacttactt
      181 ctggaatact ttggtgactt tgtgtttctt cgtggctaaa tgaatctttg acacaaggga
      241 caccaggaca ctaatttctg ctttcaactt actcctcgaa tatttggtga ctttgtgcct
      301 tcgtggttaa ttaaatccgt gccacaaggg cactaagaca ctagtttttt gctttcaact
      361 tacttctgga atactttggt gactttgtgt ttcttcgtga ctaaatgaat ctttgccaca
      421 agggacacca ggacactaat ttctgctttc aacttactcc tcgaatattt ggcgactttg
      481 tgccttcgtg gttaattaaa tccttgccac aagggcacga agacactata atcttgctca
      541 ttcttgcgat ttgatggcct ccagcctttt caacaaaggc ggatgactat aatggaaaaa
      601 tacataggcc gggtgcggct ccaggtttga caaattatca gcgctcagtt tcttcaacgc
      661 ggttttcaga aattcgccct cgaaggtggt agccgcgtag ttatctgctt caaactcatt
      721 tttcctgctc agcatgttca taaatatccc cgtaattcca ctaataggtg aaaacaaaat
      781 ggcgaaggca atcagattca ggtgaatggc aagttggttt ccacctaatg caatgctcag
      841 gtttgcattg aaaattagat acgacatgat aaacaatgta attccaacct gaattgttga
      901 agtgagatat ccccaggtga tatgtttctt tttataatga cctacctcat gcgcaaagac
      961 cgcgaccaat tcatgaactg agtgattttc gatcagcgta tcgaaaagaa caactttctt
     1021 cttccttcca aatccagaaa aaaatgcatt ggcttttttt gatcgttttg atccgtccat
     1081 aacaaaaata ttcttcaaag gaaatccgac cttattacta taatcctgga ttgcctggcg
     1141 aagttctcct tcttcaagag gcataagttt attgaataat ggcagtatca gcgtggtata
     1201 aaagaaattc atgaaaaggg taaataaaga aactgctgcc cagaaataaa tccagaagtc
     1261 agggccaaag gtttgaataa ggtaaattaa cagagcgagc aatggacctc caagcaatat
     1321 tcccagcagg tatcctttca gcttatcttt gatgaatagt gatggagtaa ctttgttaaa
     1381 accaaatttc tcttcaatca caaatgtgcc atatagctga aacggtgtcc caagaatatc
     1441 tgagacgata aatatcaaac caaaaaatgc aagcgataat agcaaaggat tttcaatgta
     1501 attccgcaag tgtccatcca accagccaaa gatgcctata gcaattactg taaaacttaa
     1561 aataaaagaa aaaaatgatg accagaatga gaatctggta ttgaccttca aatattcctg
     1621 agacttctca tacttctcct gatcatagta gtctttcatt ggttcaggaa cctttttctt
     1681 ctgcactttg atattcaaaa aatccaggat cttgccaaag acaaagctaa tggtgattat
     1741 tataagtatt aataagagaa gggtttgggg actaaaatcg gtcatcccgc aaagttaagg
     1801 caaaactagc gacgacaagg tatctgccta acattcttag gcggcttgcg tggatctcct
     1861 gtgaagaaag aataactgat tgctatctca tgggctccac ccgcgctggc tcccaactga
     1921 ctaatggtaa aatcataact atagccaata tacaaggcat cctttggtgc ttttttttca
     1981 aaacctacca tgaataccaa tgcttcatta ttggtgaagc catcaaattg cttataagga
     2041 acaccgcgat accataaccc aaaaagtacc ggttcataag taaagtacaa accaatatca
     2101 agttgagaga atatgccctg ttttctgtat tgaatagttg gagccaaact tctctcctgc
     2161 ggagtagcat aaataccctg gcccattaca cccggtttaa aaaagaattt atagccggcg
     2221 tgaacagaat acttaaccgg taacttgctg ctttccccaa tcaatgattg attaggtcgg
     2281 ttcatatgaa aggctgatac tccaagccag atattttccg aataaagtaa tcctccgaat
     2341 cccaggtcca aaaaactttt ccgggcaccg gtattaaatg tttccgcggt gggcacactg
     2401 ataaaaccat tttgatcata ttgatctccg aaggtgagtt ttccaaaatt aacgtcacga
     2461 aacacataag caacatgaac tccggctctc actgtgaaaa ttttagagat tggcaattgg
     2521 taagaatact gcaaaccaat actgttggag cgaagtccgg ccagaccttc cctgtctcct
     2581 gtaattatta ttccgattcc actattttga tcatccaaat aatgatcagc gtaggcagac
     2641 aaagtcacaa aattcgcatc gatggcaggc cattgagttc tgaagttggc tccaactctt
     2701 gtccaatttg ttgaacctac gaaggcaggg ttgaggtata agggtgcgga atagaattgt
     2761 gaaaattgag gatcctgagc tttaacaggc tttgacgaaa acaagattac cgacatcact
     2821 ccaattagag taataatcga attaatggaa gcaaatcggc taaatttttg cttatgcatc
     2881 aaattaacgg ttcaatttta gcgttatcat cctgtaaaat ggcctaaaat tataaatctt
     2941 aaatataaac gaagaattat tctttattgt attattttag aaatcacttt ttgcaaaccc
     3001 ttttgaatga attgctgagc tattaataaa agcaactgaa aaccagaaaa aatgccagaa
     3061 aggatagtta aaagtgttgt attaatttgc cttgtattca tttgctgcag tgctgttggt
     3121 caagcccgga gttattccga tctcaactgg gcttttggaa attccaatca atggatctcg
     3181 ttcaataaat caaattcaaa cccggtttta ttatcgggta aaggttccca aggagttgga
     3241 ggtgcggcta cagcaagcca cccggtaact ggagatcttc tcttttatac cgatggggtt
     3301 accgtatttg atgcatcaca ccaggtaatg acaggagggc ttggattagc aggcaacagc
     3361 tctgcaaatc aaccagttgc catttgtgca gtaccgggtc tgccaaacca atattatgtt
     3421 ttcagcaact cagcagatta tataacccca ggcactatct tctacaccgt agttgatatg
     3481 aaccaatttg gaaatgctgt atttccaatg cctgcattgg gagaagtaaa tgcttccata
     3541 aaagcgaaag caaccagtct caacaatacc gctgaagcta tgattattgt gagcaagtct
     3601 gacctttccg gattttggct tatctcaaaa cgcagtggca ataatgatgc gtatgatgta
     3661 cttgatatta gcaacggttt ggaaaacatt acgggagcag gttccttcaa cctgggtgac
     3721 cctactattg gtggaaatat ttcttataat gaagcaacag gaagagttgc ggtttcatcc
     3781 caaattgaaa atagaaatat ccagattttg caatttgaca atgcaactgg cattcttgct
     3841 tttgattctg aggtcttaaa tacaggatac aataatttat ctggtgaagc catctatgat
     3901 acagaatggt ccggcaatgg taattactta tatatttcca ttcatggcgg aggcggattt
     3961 aatggtgact tgcttcagta taattacaat aatccgctca attcactaac cagcatattg
     4021 ccgtcggtca taaccaacag ttacggattg aagatggctc cggatggaaa catttatcat
     4081 ttatttcaaa gtggcgcgga agtaaggttg gccaggataa acgaaccgga tagcattgct
     4141 gcccaaacta attacgaatt aaatccgctg ggaagtatca acttcaatgg attgcaattt
     4201 ccagagttcc ttccggaata cgatttgatg attacggcgg attttacttt cgaaggatta
     4261 tgcgctaatg tgcccaccca cttttttcct gaggtaactc cggtggcgga ttctattctg
     4321 tgggattttg gtgacggcac tggagccaat gattacagtc ctattcatac atttgatgga
     4381 caggccactc catataccgt tggcatgacc gcttttgtgt caggacaatc tatgaatata
     4441 accaagcagg taaacattct tgcatttgat gtacaaatta ccctcgtagc cgacacagtc
     4501 gcctgtaaag aagagttccc tccgccgaga ggttcatcca cacctactca attttctgta
     4561 actgccagtt ttcccaatgc tcagcctact tcaattgttt ggtccaatgg agataccgga
     4621 gcaaccttaa ctcccgatag tgcagggttt tattacgtag ttgctaccct cggaacatgc
     4681 tccatatctg caagtgttaa tgtgcaggag tatggactta tgacaaatac ggcagcgatc
     4741 tggtattttg gacaagaagc tggaattgat tttaatgaca taccacctac cgcgttatcg
     4801 gacggggtta tgaccgccag tgaaggatgt actgcagtta gtgatcaaaa tggacaaata
     4861 cttttctata cagatggatc ttcagtgttt gatcaggatc ataatgaaat tgcaacaaac
     4921 ataggaggtg atccggatgc gactcaaagc gtaatggcgg tacccttccc cagcgatcaa
     4981 acactttatt acatattcac tgccgaagct gtttacggca ccaatacttt cagagtaaaa
     5041 tattccattt ttgatttgaa attgaataat ggaacgggcg atatcatcaa agataatgtt
     5101 gtgctttttg agaaaagtac agagcggctt acctcaaacg gaggttggct catccttcat
     5161 gaattcggta ataatacctt cagggcttat ccaattttag cttccggaat tggtactccg
     5221 gtgctttcaa gcattggctc cgttcatagt gcatctattg agcgcaacgg acatggatat
     5281 atgaaattat cgcaagacaa ctccaaactt gcaatagcat tatccaacaa tgacaacagc
     5341 aattttgttg agatgttcaa ttttgatcct tcgaccggtg agttatccaa ttatactcaa
     5401 ctggatttgg gagcacaagg agctattggc caggtgtacg gaattgaatt ttctccaggt
     5461 ggaaataaaa tatttgctac ggtaaaaaat tctggcagtt ccctgatcta tgaataccgg
     5521 gcagatacat tgatgctcgc atcgtttatt ggaacccttc cagccacacc atttgccgga
     5581 gaattcggtt ctattcaata tgcacctgac ggacaacttt atgttgccat taacggaagt
     5641 caaaacctgg gtgtcatctc agttcaagaa gatgtaacgc aactttccac atttaatgcc
     5701 atcgggttta cgttagctgc aggtaaacaa agtctgttgg gactgcctaa ctttgtacaa
     5761 aatttcgcaa cgcaaaccgg agtaccagga atgtccttgc catctttagc gtgtgtaaac
     5821 aacccggtag atttagtagg caatgcttct tctattattg atgaatttga atggactatt
     5881 ctaagtccct ccggcgggcc ggttttcagt tctcaaaacc aaactgatac atttactcca
     5941 actgttgccg gaacttatac cgttaacctg cgtgttttca atcgttgtgg cctggaccaa
     6001 tcattttctc aacctttgga tgtaattgac aatcccccgc ctcctgattt gctgagcctg
     6061 gaaataattt gtgatgacaa tggcgtaact cttcaggcat taatagttga tgatcctgcc
     6121 cttacctatg catggtccac cggggaaacc tcaaaagata ttactgttct gcaacaaggt
     6181 aattttacgg taacaatcac aaatccacag ggatgcacaa gttcggccac agtacttgtt
     6241 cttgatgcca gaccgcaggt agaacttgga cccgcactga ccgtttgtca gggagatttt
     6301 gttactccgc taaatgctgg cgttaccggt gcaacttatg cctggaccct caatggcggc
     6361 agtccttctt ccgctcagac aagagctgta gatacttccg taccaggagt gtttaagttt
     6421 gtcttgtttg ttcaggatcc agcccctttc agctgttcta caacggattc cgtaaccgta
     6481 actgttcttc ctgacccggt ggtgaatgtt acggtaacac cttcccccgg ctgtaatatg
     6541 aaccagggac aaattgatat taatataact tcaacaggtt tttattccta tacagtttca
     6601 gggcctactc cttcatttgc cagcaatttc aatgggccaa ccaactttaa ccttaccaac
     6661 cttgcagttg gcttttacaa catcgtggtt acggacgaaa tcagtggctg cagttatatt
     6721 gaagtaacta gcattgtgga tgctgccacg ccatttgcaa ttcaatcggc aacctccggt
     6781 gtgggatgtg gaatggtacc ggtaacagtt aacttatccg gaacaccggc cttcccggta
     6841 acttattcat taacaaatca ggcaacagga gttataacca acggcaatgg gaatactgat
     6901 cccttcacaa cttcaaatgt cgatccaggc aattataccc tattggttac cgatgcagga
     6961 atttgtacac agagtttcgg gccatttaac gtaactgaag gtccaaaggt cactgcggaa
     7021 cttgtcctgg ataattgtac taatcctgcc acgcttacgg ttattacgaa ctcctcgaat
     7081 ccggtgtata gctggaccgg acccatttta actccaagtc ccacatcaaa taaagccact
     7141 gtagaaattc ctaaccttac tgcaacttat tcggtaacgg ttgcagattt aggaccactt
     7201 tgcgacagca ccgtgaccat tacagttaca ccgatacctt tccaaagtgc ctcaataact
     7261 tccgggggta catcctgtga tggacagcaa accctaaccg ccaacgtgac tccacctggc
     7321 aattattcat tcctttggat tcgggatgga aataatctgg gtgggcaggt tgggcaattt
     7381 atgattgcta cagtttcggg agagtatacg gttagagtca ggaatcagca atcaggctgt
     7441 gatttggtta ctaacccttt gaccgtggaa atttttgagc caataacagt ctccattaat
     7501 tccggacctc tgtgcgacaa cggtgaaccg tttaccttga aggcagatgc aattacaact
     7561 gccgatactg ttatatatga atggtcaaga aatggaagtg ttattgcggg gcaagttgca
     7621 gattctctgc aatcaacaac aaatggaatt tatgaagtga cagcaaccac cgaagatggc
     7681 tgttcagttt cttcatccta cacggttacc ctgtcaccgg taaatgttat tactctaccg
     7741 gaggcttcta ctatatgccc gctggattcc gatccattat tgaattcagt agaacttgat
     7801 gctggcacag gcttcactca atacatgtgg tttgataata atgggaatat agtttcaaac
     7861 aaccaggttt acacagcatc cattgagggt aaatatacgg taataggcac caatcagttt
     7921 gggtgtacta ctgaagcatc aagtgttgtg atcgaagatt gcgttccccg cgtattcgga
     7981 cccaatgcat tcagacctaa gggcacaaat gatgaattct tcttattcac caagtatatt
     8041 attgattttg agatatttat atacaacaga tggggtggct tggtttatca ttccaaccag
     8101 gaggatttta gatgggatgg aatattcgaa gggagattgt tacccgccgg gacttatgct
     8161 tataaagtac gattcaaaag tgaatttaat ccactgcggg gaatgatgga aaagcatggc
     8221 ggagttacat tattgcgtta ataatccgat atttgtctta tgaagaaaat atcttacatt
     8281 ttactgattc tgattttttc agcaccaatc atcaatgcgc aagcacaggt tcttaaaacc
     8341 aatctgacaa tcacagttct ggatgacaat ggcaatatcc aggaaggcgc tacagtaatc
     8401 ctttacaaga atgaaacaga ttatcgcgaa agtaagaatc ctgccattga aaagcaggtg
     8461 acggataaga agggcagagt caaattcaaa gatttggata cgatggccta ttttgtggaa
     8521 gtcaggaaag gcgatcttaa caatgatgga ttaggtgctc agaccggtga acttgtggcg
     8581 ggaagaacga ataaggcaaa tattattata cagtaatccg ttcctcccga gctttatgcg
     8641 tataccgatc caggtccgtt attcagattt tgatatgatg ggccacttga ataattctgc
     8701 ctatgccaca tattttgagc tggccaggct gcaatttcta atgtccttgt ttgaacaaaa
     8761 ctcctttaaa tggacagatg tagtagcgaa ccttaatatt gactatcgaa taccggttgt
     8821 acctcagagt aaacccatca tgtatatctc ttgttccaga attggtaatt ccagttacga
     8881 tcttgagtat aaggttgttg aggaaactga tgaaacacac ctttatgctg aaggtaaaac
     8941 ggttcaggtt tgtgtggatc caaagacaca gacatcacaa aaattaccgg atccggcaaa
     9001 ggaaattttg agtaatgttt tggttcctaa aaaagaataa ccccggattt tggggccggg
     9061 gttaactatc aaccttaaag tttggggtaa atactaccag agataatact cattcaaatt
     9121 tactaatcac tgtcccctaa actttgcctt ttccctatta ttagcatcta tgtaagattt
     9181 ccgtacgatt tatccaaaat gaagtttatt cctccatata attataatct gaaaaaaata
     9241 tagcttttaa cctggattaa aattgagcgg aattcatgta ataaataatc agtttattat
     9301 atattcctga gtgaaaatag taaaaactgc tctattttga ggtaagaagc aaaattcctg
     9361 aagccgctac aaccatccaa aacacataat cagacaaggc cggaataacc ataatgtcca
     9421 atttatccag caccggtatc aaaatcataa gaattgaaat caatctgagg atagaacgcg
     9481 ttttattgct cataattatt ttagttagtt gggcacaaat taataatttg gagtctaatt
     9541 cttcaaacta atcgcggaaa taggcactga tattgaaaaa gccgtaacca ttttgaaaaa
     9601 tggcggcctg gttgcaatcc caactgaaac ggtatacggg ctggcaggta atgcattaga
     9661 tgagagggtg gtttcaagaa ttttcagcgc aaagaaacgg cctcaattcg atccattgat
     9721 agttcattgt tcaagtattg gccagataag ccattatgcc accctggaga atccacaggc
     9781 caggctttta gcagaaaaat tctggccggg ccccatgaca ctggttctgc caaaaaaaga
     9841 cgttgttccc tatttggtaa ccagtggaat ggatacggtt gctatgcgga taccagcaca
     9901 cgaaatgaca ttacatttat tacggcaact tgattttcca ttggttgccc cgtccgccaa
     9961 tccttttgga tatgtaagtc cgactactcc ggaccatgtg aatgacaatt tgggtgatgt
    10021 cgttgattat attcttgacg gcggaccttg ccaacttgga attgaatcaa caataatttt
    10081 gtttgacaat aaaattccga ctgttctgcg aagcgggagt ttatcggtgg aaaaaataca
    10141 aacaattatt ggagagataa atgtggtcac gcattcttcg tcaaatccct ctgcacccgg
    10201 gatgcttaag tcgcattacg ctccaggaaa gaaattagtg ttagggaata tagaactttt
    10261 gcttggaatg cccgattatg atcgcgtcgg agttctttct ttcagggaga aatttgaaca
    10321 ccccaagatt gtcagccaga tcatattatc agaaaatggc gatttggaag aagccgctac
    10381 caaccttttt tcaggtcttc gatctctgga taaaatggat attaatttta tacttgcaga
    10441 aacggttcct aatcatggct taggccgggc aataaacgat cgacttaaac gcggagcatc
    10501 tgataaataa tgaaaacggt agattcaata aatttcaaaa ataagaaagc ccttattcga
    10561 gtagacttca atgttccctt aaacaatgaa ctacagatta cggatgatac cagaattaag
    10621 tcagctattc caacaattaa taagatactt tctgatggcg gatcagcaat cctgatgtct
    10681 cacttgggaa gaccaaaaga cgggccaaat ccaaagtatt ccctcaaatc tctattaatc
    10741 gatttgcaat cgcgcttcag tggtgcggtt tactttagtt ccgattgtat cggcgaagaa
    10801 ccgcaaaagt tagccaggaa tttaaaaccc ggggaattat tattgttaga aaatcttcgc
    10861 ttccatccgg aagagaccaa aggagaattg agcttcgcca gacaattagc tgccttgggt
    10921 gatgtatacg taaatgatgc atttggcacc gcgcacagag cccatgcttc cacaacggtc
    10981 gtagcccaat tttttcagga taaagtttgc ggatatgtga tggccgctga aattgaaaat
    11041 gcagatagag ttttacatca ttcggaaaaa ccttttaccg ccgtaatggg tggcgccaaa
    11101 atttccgaca agattttggt tattgaaaaa ctgctggata aagtgaataa tctccttatt
    11161 gggggtggga tggcatatac attctttaaa gctttgggtg ggaatattgg caattcctta
    11221 cttgaacctg ataagctgga tttagcaaaa gaactgatag gaagagcaaa agcaaaaaat
    11281 gtgaacctgg aactcccgat tgattccatc attgcaaata aatttgagaa taacgccgaa
    11341 cataaagttg ccaaaaacat ttccattcca gatggctgga ttggaatgga tatcggtccg
    11401 gagtcccgga aagtattttc taacattatc attaactcca ggacaatttt atggaatggc
    11461 ccaatgggtg tttttgaaat gcataatttt actgccggaa ccattgaaat tgcagaagcc
    11521 gttgtttcag ccacagaaaa aggcgcttat tcattaattg gaggtggaga ttctgcttct
    11581 gcaataaata acctgggatt tggagacaag gtttcctacg tttctaccgg cggaggtgca
    11641 ctcctggaat atatggaagg caaattactt cctggtatcc ttgctctcga ataaaatgag
    11701 atcaatccgg aatcaacgga agatttgcct gcgacagaat cattgacctc tttataagaa
    11761 attaaagcgg aatttctaaa gttggaagat ggattggaaa ttaatttgga tgaaataatt
    11821 aaattcaatg gcctggcaat acctgatatt tgctgatgtg gctttaaatt tgagttaata
    11881 gtgaatgcaa tgaaagttag aaattggttt gccttactat tatttatctt tccccaggat
    11941 acagccgggc aagtttggaa attatttgaa acggaacaaa tccatcagaa gcgggctgaa
    12001 tccggaaaga attattttcc atttctcaat gagtcttctt tcgcgattgg tctgtacgaa
    12061 ttaaagtcta aagtaattga taatcagcca gttcaccatg aagatgaagt ttattttatt
    12121 gtatccggaa aagcaaaact agtagccggc gacagcactt ttgatgtctc cgccgggagt
    12181 atcgcctttg taagagctgg tgtttcgcac aggtttgttg aaattgaaga agacttctct
    12241 gcacttgtta tattcaccaa aacaccaaca aaaacaggcg atgctgatgc gttggctttt
    12301 gagcttgata agttaaggca accaagaaat ccggaagaga acgtctggaa ccagtttctt
    12361 tttgtttcaa ccatacgaat ggggttgtac cttcttcctc aatctctggg tggcgaccag
    12421 ccaatgactc ataatgtgga tgaaattaat atcgtgacaa atggtgacgc gacgctggtg
    12481 gttggggaag aaaaaattcc ggtaaaaatg ggatcaattg tttgggtgaa ggaaggcaac
    12541 cctcattcct ttacaaatct aagtgaagat tttgatgtcc tgattgcctt tcatatcaaa
    12601 ccctagttac cgaaaaaccc ttcttccggg gcatccgaaa atgcatcagc cctaaacctc
    12661 catgaaccat ccacttgttt gcgtgcaacc gtaataaatt taccaatcga tcttccctgg
    12721 acaccctctt tattcatcct gtctaattta tagtagccta tatc
//
LOCUS       DASWHX010000067        17220 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_2272673, whole genome shotgun sequence.
ACCESSION   DASWHX010000067 DASWHX010000000
VERSION     DASWHX010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 17220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17220
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2272673"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..640)
                     /gene="trpA"
                     /locus_tag="VGA21_04595"
     CDS             complement(<1..640)
                     /gene="trpA"
                     /locus_tag="VGA21_04595"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF012511.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEX9649814.1"
                     /translation="MDQVRNILVNSSLNSTLQRARLLSIYFTAGYPNAADTSQILLSL
                     QEGGVDIVEIGFPFSDPIADGPVIQKSSQRAIQNGITLKKIFSQLEQVKDEVRIPVIL
                     MGYLNPVLRFGMEQFCTKCNQTGVSGVIIPDLPISEFMTDHFEIFKQNNLSAIFLVTP
                     TTSEERIRLIDSISSSFIYAVSDSAITGVKKDLTEHQLQYLKRLSEMNLKHPV"
     gene            complement(627..1832)
                     /gene="trpB"
                     /locus_tag="VGA21_04600"
     CDS             complement(627..1832)
                     /gene="trpB"
                     /locus_tag="VGA21_04600"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013453187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEX9649815.1"
                     /translation="MFQLKTCTGQYDVDENGFYGSYGGSYIPELLHNNIQELKENYLR
                     IISQHKFKDDYEKILTDFVGRPTPLFFAEKLSNQTGARVYLKREDLAHTGSHKINNAV
                     GQVLLARALGKTNIIAETGAGQHGVATATVCALAGIPCKVFMGKIDMKRQQANVDRMK
                     MLGAEVLPVSSGAMTLKDATNEALRYWISHPYDTHYLIGSVVGPHPFPDMVARFQSVI
                     SWEIKKQLTKSEGRENPDYIIACVGGGSNAAGAFYHYLDRDEVALVGVEAAGEGLMSG
                     RSAATLKLGKAGILHGSKSFILQNNDGQIAEAHSVSAGLDYPGVGPLHAYLVEKKRIK
                     LLSATDSEAIAAGINLSRLEGIIPAVESAHALAALVKLKFKPNDVVVLNLSGRGDKDL
                     EIYKSYGPG"
     gene            complement(1819..2463)
                     /locus_tag="VGA21_04605"
     CDS             complement(1819..2463)
                     /locus_tag="VGA21_04605"
                     /inference="COORDINATES: protein motif:HMM:NF012900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEX9649816.1"
                     /translation="MSDLKLKVCGMKDVNNIRCLSQLPIDFIGFIFYKHSPRHVETDD
                     KEFVETVQTMPNSIKKVGVFVNEKPQKILETGSRLSLNVIQLHGEESPATCKLLKKEG
                     FLVIKVIKIENNLPIELEAYENICDYLLFEAKGLMPGGNNKTFDWKILEAFSFQAPVF
                     ISGGISFSNFSEALKIPIDNLFALDINSAFEIYPGFKDVSKINKLLSKLKNVPA"
     gene            complement(2456..3271)
                     /gene="trpC"
                     /locus_tag="VGA21_04610"
     CDS             complement(2456..3271)
                     /gene="trpC"
                     /locus_tag="VGA21_04610"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007661310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HEX9649817.1"
                     /translation="MAILDKILKFKRREVAEKKSLITVKYLENSIYFNSQTLSLKKYV
                     LRADKSGIIAEIKRKSPSRGILNTNVSMEQTSIGYMQAGASAISVLTDSNFFGGFADD
                     LTTARKFNFCPILRKDFILDEYQVIESKSIGADAILLIASGLDSGKLKSLASLAHSLG
                     LEVLFEIHSEKELKKIGEQEIDLVGINNRNLDTMETNLSKSEELVSKIPEGLTKVSES
                     GIKTPDDVLHLKELGFHGFLIGEQFMIHPRPEEACCEFIEKVLVKQKNPISHE"
     gene            complement(3258..4283)
                     /gene="trpD"
                     /locus_tag="VGA21_04615"
     CDS             complement(3258..4283)
                     /gene="trpD"
                     /locus_tag="VGA21_04615"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009184002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEX9649818.1"
                     /translation="MKELLNELFSYKTISRENAYGVMLRIAKGDFSNSLVASFLTIYN
                     MRSITLDELAGFREALQEMCVPVNLSAYDPIDLSGTGGDGKNTFNISTLASFIVSGAG
                     QAVVKHGNNGVSSICGSSNLLSHFGYEFSSDKDTLRRSLDAAGICYLHAPLFHPALKN
                     IATVRKELGVKTFFNMLGPMINPASPKKQITGVFSLELARLYGYLYQNTDKKYQIIHS
                     LDGYDEISLTSDFKIIHAGGESIISPGMLGFDQISPKAINGGNSIEKSADIFLKILAG
                     KGTTEQNNVAISNAGLALYCGKELSSFTEGISIAKESLESGKALESFKKFLSPEKTFM
                     HISHGNT"
     gene            complement(4280..4855)
                     /locus_tag="VGA21_04620"
     CDS             complement(4280..4855)
                     /locus_tag="VGA21_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017733649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEX9649819.1"
                     /translation="MKILVLDNFDSFTYNLVDIIRKGNHNASCSVFRNNEIILDDCRN
                     YDKILIGPGPGLPNSAGIVPQVIQELGPCKDILGICLGHQGIAEAYGAQLFNLDSVLH
                     GITNKIKVIDSAELLFKDLPAEFNAGHYHSWSVLPESVGDNLVVTAIDENGLIMGLRH
                     SQFSVRGLQFHPESIMTEQGEKIIMNWINGQ"
     gene            complement(4852..6264)
                     /locus_tag="VGA21_04625"
     CDS             complement(4852..6264)
                     /locus_tag="VGA21_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009580663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HEX9649820.1"
                     /translation="MKKNLKIIQKRILADTLTPVIIYLKLRDLYPYTLLLESSDYESS
                     VNSKSIICASPISRFVVKNNTLNVESPGGDKIFHQFQNKRELLDLLTESFSSFHVDSK
                     ENEIGCNGFFGYMTFDSVKFFEPVSFNKPCDENSIIPEMIYAAYRFVIVADHFKNEIL
                     LIENLIGNQESELEALIAIIRNRNFPMYTFQSNGEEKSYFRDADFLEIVSKGKQHCQL
                     GDVFQVVLSRKYSRPFLGDDFNVYRALRSINPSPYLFYFDMGEFRIFGSSPEAQLTIK
                     NNKAAIFPIAGTYERNGDPEFDKKEAEKLLLDEKENAEHVMLVDLARNDLSRSSSNVS
                     VESFKEIQYFSHVIHMVSRVTASLGTDEFNFDIVADTFPAGTLSGAPKIRAIELISEL
                     EDSPRGYYGGAIGYISFNGEYNHAIMIRSFLSKNNQLTYQAGAGIVAKSNPDKELDEV
                     NHKLNALRQAIENATKINYL"
     gene            6634..8988
                     /locus_tag="VGA21_04630"
     CDS             6634..8988
                     /locus_tag="VGA21_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015333521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HEX9649821.1"
                     /translation="MNRILLAAIFLAFVTISRAQSYLPRWNDSRVKVETLNLGSYAFN
                     LSDVELLESPFKEAMQVTAAYLLKVEPDRLLSQFRSNAGLEPKGEIYGGWESAGLAGH
                     SLGHYLSSCSMYYAVSKDTRYLERVNYMVEQLEETQKARGTGYVGAIPNEDSVWAEVK
                     KGNITARDFYLNGAWSPFYTLHKIMAGLLDAYLYTDNQKALEINIGIADWTGDLISGL
                     SDSLRWKMLICEYGGMNETLANTYAFTGNKKYLELSRKFQDHHILDSLGMEKNVLPGK
                     HSNTQIPKVIGSARRYELTGNSQDIVTTLFFWNVMVNMHSYANGGNSNYERLSAPGRL
                     NDELTDNTTETCNTYNMLKLTTHVFAISPMSWQMDYYERALYNHILASQNPNNGMVCY
                     FVPLRMGSRKRYSNEFNNFTCCHGSGMENHVKYGEAIYSHSGDGKLFINLFIPSKLSW
                     HEKGVIIHQDTKFPEEDKVRIRIEAERSISFPILIRRPEWVTDNFRVSINGKEIKEYA
                     GSGNGYLHLEQKWKSGDEIIVELPMKLYSEPMPDNPNRVALFYGPVLLAGNFGETEPD
                     PEIGIPSFITGNPDVETWLDKRDEKSLLFSTGKMSDSSKVNLIPFYETIDEFYSVYWD
                     VYHPIRWKEELRRMEDEKQRKLNIENRTIDMLIIGDKESEERQRFEGVEVRTGENHRK
                     SFIRTNRNGSFSFISKIKQGSENTLHCIYWGEDNRGRAFDILIDGELLASVELVIYKG
                     SKFHEITYPIPPSLTEGKTEVKIAFQAKENNIVGPVYGIRMLGN"
     gene            9222..11735
                     /locus_tag="VGA21_04635"
     CDS             9222..11735
                     /locus_tag="VGA21_04635"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HEX9649822.1"
                     /translation="MNKNHNKIQEPDTIKYSCPMKCEGDKTYSKPGKCPVCNMNLIPF
                     NEQKSEGNTCCHHEPGHQVHLHDDHDSHCHMEEKPVQSSGKNEQREGAGKYYCPMRCE
                     GDKMYDEPGDCPVCGMHLQQEVKLSSPKSKTRKTTYTCPMHPEVKSDKPGSCPKCGMD
                     LVPEKPDEAAESDEEKSYKKTLRKFWLAVAFTSPVFVIAMSEMVNIDLENIASRKIWG
                     WIQFGLSTPVIFYSSWVFFKRGWSSIRRRSPNMWTLISLGTGAAYFFSIIALIAPQIF
                     PPQFRDIQGNVHLYFEAATVILTLVLLGQVMELRAYSKTNSAIKSLLNLVPPVARKMV
                     NGTEHEIPLAEVKVGDTLRVKPGEKIPVDGTINDGNAVVDESMITGESIPADKTPGDK
                     VTGGTINGKSSFEMKADKVGSETLLAQIIEMVNQAGRSRAPIQKVADIVAKYFVQIVI
                     AIAIITFVVWSIFGPEPAYVYAFVNTVSVLIIACPCALGLATPMSIMVGTGRGAQSGI
                     LVKDATAIEEMNKINVLVIDKTGTITEGKPRLKNYKSLGAYEEKAILKFAASLDAYSE
                     HPIADAIVKGAKEANLELEKLEEFESVSGKGVQAMFRGKRIGLGNKRLVQDFNVAIDE
                     EVSKLVEGWQLAGETVMFLMVENKIEGIISVADKIKSTSAAAIKSLQEMGVKVYMLTG
                     DNEFTARAVANEINLDGYESECLPEDKHKKVKNLQDQGFKVAMAGDGINDAPALAQAN
                     VGIAMGTGTDIAMQSAQITLVKGDLTGIARAYELSKKVMRNIKQNLFFAFVYNTIGVP
                     LAAGVLFPFFGVLLSPMIAAAAMSFSSVSVIGNALRLKQ"
     gene            11871..12218
                     /locus_tag="VGA21_04640"
     CDS             11871..12218
                     /locus_tag="VGA21_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013408123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HEX9649823.1"
                     /translation="MEKSFSFAVKVVELVKILSKEKNEYVMTKQILRSGTSIGANVSE
                     GLGGSSKKDFVSKMYIAYKEAKETIYWLNLLHATNYLNRDNFNSFRDDCEEIIKILYS
                     ILKTSRKNINSGN"
     gene            complement(12326..13549)
                     /locus_tag="VGA21_04645"
     CDS             complement(12326..13549)
                     /locus_tag="VGA21_04645"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HEX9649824.1"
                     /translation="MKCKFAVLSLFYVLLAITAVAQEKDSTKGYRKYDLLPAISYAPE
                     TGLTLGIIGYRYLRLGKKYPTRQSNIDFVAVYTTRNQIIVESNWDIFTDGDKFRFRGF
                     AGYNLYPDRNYGLGNNASAFIREYELDNALKTDSTDLNYLNFSIQRITFKPVFLMKLR
                     DKIYGGVRFDLEYQYAMKIIPDQYTFLREGSAISLMQNNNTGFRSGVGLALVYDSRDN
                     ILNARNGIFIDYSALFYGKLLGSDYTYSYTRIDARKYFNPVKNHSIALRANFIFSRTD
                     EMVIPMRGLARVGGHDFMRGYFRGTFQDKNLASFEAEYRLPFWSDDVDAPLYKIWKRL
                     GMVAFVSGAQVYGYSGNFAFDKFNYAAGTGLRVLFNPESRVNIRIDYSWGLSKDSAGP
                     GSRQTGLYFFLSEAF"
     gene            13881..14927
                     /locus_tag="VGA21_04650"
     CDS             13881..14927
                     /locus_tag="VGA21_04650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649825.1"
                     /translation="MNTTRTICTIFSLLISVLLINCSGERSNQDEQSEQPDVQAMLEV
                     KEWPVPWEDSRPRDPYVAPDGKVWFCGQAGNYLASFDPATEEFKRFEVPERSNPHNLI
                     IDDEGFVWYAGNLNAHIGKLDPADGSITQFPMPDPEARDPHTLVFNQEGNIWFTVQGG
                     NFIGYLNTTTGEVKLVSVPTPKARPYGIKMDPQNQPWVVLFGTNKLATIDPSTFELKE
                     IELPDSNSRPRRLEIKEGLIYYADYSKGYVGRYDTNTGSFAEWPLPGGRDSRPYGTAM
                     DHQGIIWIAEAGLDPNRLVGFDPGNGSFVGITEIPSGGGVIRHMYFYPQKREIWFGTD
                     TNNLGRAVVSAAGS"
     gene            complement(15067..15819)
                     /locus_tag="VGA21_04655"
     CDS             complement(15067..15819)
                     /locus_tag="VGA21_04655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649826.1"
                     /translation="MKKIIVGCIKAGLVMIFLITGTTVSAQPAQIGLFSDSVMRTKTI
                     RIENSLVITDYGSFTPDVIRYIEFVDKDITQRDLYKQLKALNVRFYFSPIFLAVVKLH
                     NNKRFSGYVLAANEGKVTLFRRWTKKTFPLSDIEHLTIYENKSGEFWGGFGYGVIGQL
                     VAGATLIAITAPTGEFLFFAAIGMVTGNLVAGLAVGSIVGHAIMTSKVNASKKRYVVT
                     GENLKEIESILTKHQTMKNESTKWMYKKMTAQ"
     gene            16073..16732
                     /locus_tag="VGA21_04660"
     CDS             16073..16732
                     /locus_tag="VGA21_04660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649827.1"
                     /translation="MKKITIHLCLLIGGSLLTLNLFGQDRSNDFLFPGKNKFQATIST
                     GIPYVALIEGTYGITRRFAAGIIVGTTPNVIGYGIRLRAQLYQPNENFRVYFRMPVFY
                     YASTKNFGNEPWMLSWPVIAAEWKIEDLVMLSFGVGVVEAHCIDALLAGMNHHDEPID
                     MGFEGGFWNTVNGGIAWPLSKSLTIQAETSIVLEGWKIAGSNYVGGPPVIVVLGLTKN
                     F"
     gene            complement(17004..>17220)
                     /locus_tag="VGA21_04665"
     CDS             complement(17004..>17220)
                     /locus_tag="VGA21_04665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649828.1"
                     /translation="RFQSYLNTGQQGDTYLKIIGENQYEWGFEGRVKYLITYNPDAQT
                     WLETGEYSADNGTSWNKFIEMNLKKVK"
BASE COUNT         5158 a         3497 c         3547 g         5018 t
ORIGIN      
        1 atacgggatg tttcagattc atttcagaaa gtcttttcaa gtattgcaat tgatgttctg
       61 ttaaatcttt tttcaccccg gtaatggccg aatcagaaac agcataaata aaggaacttg
      121 agatagaatc gattaatcga attctttctt cagatgtagt tggggtcact aaaaaaatgg
      181 ctgacagatt attttgtttg aaaatttcaa aatgatctgt cataaattcc gatattggaa
      241 ggtcaggtat tattactccg gaaactccgg tttgattaca ttttgtacag aattgctcca
      301 ttccaaaccg gagcacagga ttcagataac ccattagtat tacgggtatt cttacttcat
      361 ctttaacctg ttccaattga ctaaagattt tctttagtgt gattccattt tgaattgccc
      421 gctgtgaact tttctgaatg acaggtccat cggcaattgg atcactgaag ggaaatccga
      481 tttcaacaat atctacaccg ccttcctgga gagatagcaa aatttgtgaa gtgtccgctg
      541 catttggata gcctgcagta aaataaatgg aaagcaatct cgccctttga agagtagagt
      601 taagggaaga atttaccaat atgtttctaa cctggtccat agcttttgta tatttccaaa
      661 tccttatcac ccctgcccga tagatttaat accacaacat cgttcggctt aaacttcagc
      721 tttactaaag ccgccagcgc gtgagctgat tcaactgcgg gaattattcc ctccaaccga
      781 gataaattta tcccggctgc aatcgcttca ctatctgttg ccgataacaa tttgatgcgt
      841 tttttctcaa ccaggtatgc atgcaatggt cccacgcctg gataatccaa tccggcgctt
      901 acagaatgag cctctgctat ttgaccatca ttgttctgca ggataaaaga ctttgagccg
      961 tgaagaattc ctgctttgcc cagttttaat gtggcggcgg acctgccact cattaatcct
     1021 tcaccggctg cttccactcc aaccaaagct acttcatcgc gatccaggta gtgataaaaa
     1081 gctccggcgg cattgctacc cccgcccaca catgcgatga tataatctgg attttcccgg
     1141 ccttctgatt tggtcaactg tttttttatt tcccaactga ttacagattg aaatctggca
     1201 accatgtcgg gaaaagggtg gggacctaca acagacccaa ttaaataatg tgtgtcataa
     1261 ggatgactta tccaataacg caaagcctca ttggttgcat ccttcaaagt catagcgcct
     1321 gaagaaaccg gtaatacttc agctcccagc attttcatcc gatccacgtt tgcttgctgc
     1381 cttttcatat caatcttacc cataaatact ttgcagggta tgcctgccag cgcgcatact
     1441 gttgcggttg caacgccatg ctgcccggcc cctgtttccg cgataatatt ggttttcccc
     1501 aatgctcgcg ccagcaaaac ctgacctact gcattgttta ttttatgtga tccggtatga
     1561 gccaggtctt cccttttcag atatacgcga gctcccgttt gattactcaa tttctccgcg
     1621 aaaaacaggg gagtgggtct gccaacaaag tctgtcaata tcttttcgta atcgtcctta
     1681 aacttgtgtt gtgagataat ccttagataa ttttctttga gctcctgaat gttgttatgt
     1741 aaaagttccg ggatataact accaccataa gaaccataga acccattttc atctacatca
     1801 tattgtccgg tgcaggtctt aagctggaac attttttagt ttacttagca gtttattaat
     1861 ctttgaaacg tctttaaacc ccggataaat ttcaaatgca ctgtttatgt caagtgcaaa
     1921 taaattatca attggaattt ttaatgcttc tgaaaaatta ctgaatgaaa tccctccact
     1981 tatgaaaaca ggtgcttgaa aagagaatgc ttccagaatt ttccagtcaa atgttttgtt
     2041 attaccaccc ggcattaatc ctttggcttc aaacaagagg tagtcgcaga tgttttcata
     2101 agcttctaat tcaatgggca ggttattttc aatcttgatc acttttataa ccaggaaacc
     2161 ctctttcttt agaagcttgc acgttgcggg agattcttcc ccgtgaagtt gaataacatt
     2221 taatgacagc cgacttcccg tttcgaggat tttttgaggt ttttcgttta caaaaacccc
     2281 cacttttttt atgctgttcg gcatagtctg aacagtttca acgaattcct tatcatctgt
     2341 ttcgacgtgt ctgggagaat gcttataaaa tataaatcct atgaaatcta tcggaagctg
     2401 tgataaacaa cgaatattat tgacatcctt cattccgcaa acttttaatt ttagatcact
     2461 catgactgat cggatttttt tgtttgacaa gaactttctc tataaattca cagcaagctt
     2521 cttcaggccg gggatgaatc ataaattgct ccccaatcag gaaaccatgg aatcccaatt
     2581 cttttaagtg taatacatca tccggagttt taattccact ctctgaaact ttcgttaaac
     2641 cttcaggaat ttttgaaacc agctcttccg acttagataa atttgtttcc atggtatcca
     2701 ggttgcggtt gtttattccc actaaatcaa tttcctgctc gccgattttt tttaattcct
     2761 tttctgaatg aatttcaaat aaaacttcca aacccaggga atgggctaaa gaggcaagac
     2821 tctttaattt tcctgaatca agacctgatg caattagcaa aatggcgtca gcgcctatac
     2881 tcttactttc tattacctgg tattcatcaa ggataaaatc tttacgaagg atggggcaga
     2941 aattaaattt tctggcggta gtcagatcat ctgcaaatcc accaaaaaag tttgaatctg
     3001 ttaagactga aatcgccgaa gcaccggcct gcatatatcc aattgaagtt tgctccatgg
     3061 aaacattggt gttaagaatt cctcttgaag gagatttgcg ttttatttca gcaataattc
     3121 cggatttgtc ggcccgcagg acatattttt ttaaactcaa tgtctgagaa ttgaaataga
     3181 tggaattctc cagatatttt acagtgatca gagatttttt ttccgccact tcgcgccgct
     3241 taaatttcaa tattttatca agtattgcca tgagatatat gcataaaagt tttttctggt
     3301 gaaagaaatt ttttaaatga ttccaaagcc tttcctgatt ctaatgattc tttggcgatg
     3361 gaaatgcctt ctgtaaaaga actcagttct ttcccacaat aaagtgcaag gccggcattg
     3421 gaaatcgcaa cgttattttg ttctgtggtg cccttacctg ctaatatttt taaaaaaata
     3481 tctgctgact tctcaatcga attgccgcca ttaattgctt taggcgatat ttgatcgaag
     3541 cccagcattc cgggtgaaat aatactctct ccccctgcat gaataatttt gaaatccgat
     3601 gttaatgaga tctcatcgta gccatccaga gaatgaatta tttgatattt cttatctgtg
     3661 ttctggtata aatacccata aagccttgct aactcaagac tgaatactcc cgtaatttgc
     3721 ttttttggtg atgcaggatt aatcatcggg ccaagcatat tgaaaaaagt ttttactcca
     3781 agttcctttc ttaccgttgc aatatttttc agggcagggt gaaacaaagg agcatgcagg
     3841 tagcagatac cggcggcatc aagacttcgt cttaaggtat ccttatccga actgaattca
     3901 taaccaaagt ggctcagtaa atttgaagaa ccacaaatag aagatactcc gttatttcca
     3961 tgtttcacta cggcttgccc tgccccggaa actataaagg aagcaagcgt tgaaatattg
     4021 aaagtatttt taccatcgcc cccggttccg cttagatcta ttggatcata ggctgacaag
     4081 tttacaggca cgcacatttc ctgaagggcc tccctaaatc cagccagctc atcaagagtg
     4141 attgatctca tattataaat agtgagaaat gaagcaacca gggaattgct gaaatcgcct
     4201 ttcgcaatcc ttagcattac tccataagca ttttctctcg aaatggtctt gtaggaaaat
     4261 aattcattta ataattcttt cattgtccat ttatccagtt cataattatt ttttcgcctt
     4321 gttctgtcat gatagattcc ggatgaaatt gcaacccgcg aactgagaat tgtgaatgtc
     4381 tgagccccat gatcaaccca ttttcatcga tggcagttac aacaaggtta tcgccaacac
     4441 tttccggcag caccgaccag ctatggtaat ggcctgcatt aaattccgcg ggaaggtcct
     4501 tgaagagtaa ttcagcggag tcaattactt tgatcttatt ggtgatcccg tgcagtacag
     4561 aatcaagatt gaataattga gctccgtagg cttccgctat tccctgatgg cccaggcaga
     4621 tacctaaaat atctttgcaa ggtcccagtt cctgaataac ctgcggaact attcctgctg
     4681 agtttggcaa accaggtccg gggccaataa gaattttgtc ataattcctg cagtcatcaa
     4741 gaatgatttc attatttcta aacacgctac aggaagcgtt gtggtttccc ttcctgatga
     4801 tatcaacaag gttgtaagta aaagagtcga aattatccag caccagaatt ttcataggta
     4861 attaattttt gtagcgtttt caatcgcttg ccttagcgca tttagtttat ggttaacctc
     4921 atctaactct ttgtcaggat ttgattttgc cacaataccg gcccctgcct gataagtcag
     4981 ttggttattt ttgcttagaa aagaccgaat cattattgcg tgattatact caccattgaa
     5041 actgatataa ccaatcgcac cgccataata tcctctggga gaatcttcaa gttctgaaat
     5101 cagttcgatg gctcttattt ttggagctcc cgataaagtt cccgccggaa aagtatctgc
     5161 aactatgtca aaattgaatt catcggttcc aaggcttgca gtaacccttg aaaccatgtg
     5221 gattacatgg gaaaaatact ggatttcctt aaatgactcc acggatacat tggagctgct
     5281 tctgctcaaa tcatttctgg ccaggtcaac caacattacg tgctccgcat tctctttttc
     5341 atcgagcaaa agtttttctg cttccttttt gtcaaattcc ggatcgccat ttctttcata
     5401 agtgccggca attgggaaaa tggccgcttt gttattctta attgttaatt gggcttcagg
     5461 cgacgatccg aaaatccgaa attcacccat atcaaaatag aataaatacg gagatggatt
     5521 aatggagcga agtgccctgt aaacattgaa gtcatcaccc aggaaaggtc gtgaatactt
     5581 tcgtgataat acaacctgaa agacatctcc cagctgacaa tgttgctttc ccttgctcac
     5641 aatttccaaa aaatcagcat ccctgaaata acttttttct tcaccattgg actgaaaagt
     5701 atacataggg aaattcctgt tcctgatgat cgcgattagg gcttcaagct ccgactcctg
     5761 atttccaatg agattttcaa tgagcaggat ttcattctta aaatgatcag ctacaattac
     5821 gaaccggtag gcggcatata tcatttcagg aattatggaa ttttcatcac agggtttgtt
     5881 aaaagaaaca ggttcaaaaa attttacgga atcaaaggtc atataaccaa aaaaaccgtt
     5941 gcacccaatc tcattttctt ttgaatcaac atggaaggat gaaaatgact ctgttagcaa
     6001 atcaagcaat tctcttttgt tttggaattg atgaaaaatt ttgtccccgc ccggactctc
     6061 aacatttagt gtgttatttt tcacaacaaa tctgctgatg ggtgaggcac aaattataga
     6121 tttgctgtta acggaacttt cataatcaga actttccaat aacaatgtat aagggtataa
     6181 gtcccttaac tttaaataaa ttattaccgg cgttaatgta tcggctaaaa ttctcttttg
     6241 aataatttta agattcttct tcataatcag aaaataaaaa aggcttcctc agtggtgagc
     6301 aagccttttg tcggacatac ctataaaatg ctcacctgaa ccagtttgaa atccaccacc
     6361 atttttttat aatcaccatc atttctttaa cataaaaaaa gcctgcgtga ggcaggctta
     6421 tatcttatta atcaataaaa ttcccgggtc acgccttaat agttagcatt ttccaccacc
     6481 agattttatt gaacgatttc ttaaccatag tccaaatgta attggcaatt tacttaaaac
     6541 aacatttttt tagaattttt cataaaatgg aaggtctttc gctttcttat gcagattttt
     6601 cataatttca attatcacga tcaaaaacct gccatgaata gaattttact tgcagcaatc
     6661 ttcctagcat tcgtaacaat ttcccgtgcc caatcctatt tgccccggtg gaatgattcc
     6721 cgggttaaag tagaaacatt gaatttggga agctacgcgt ttaatttgtc agatgttgaa
     6781 ctactggaaa gtccattcaa ggaggcaatg caagttacgg cggcctatct gcttaaagta
     6841 gagccggaca ggttattatc tcaattcagg tcaaatgccg gcctggaacc aaagggtgaa
     6901 atttatggcg ggtgggagtc tgcaggtctg gcaggccaca gtttgggaca ctatctgtca
     6961 tcatgctcca tgtattacgc ggtatcaaaa gatacgcgat acctggaacg cgtgaactat
     7021 atggtggagc aattggaaga aactcaaaaa gcgagaggta cggggtatgt gggcgccatt
     7081 cccaacgagg atagcgtttg ggcagaagta aaaaagggaa atataactgc ccgtgatttt
     7141 tatctgaatg gagcctggtc gcctttctac actttgcaca aaattatggc cggtctgctg
     7201 gatgcgtatt tatacactga caaccagaaa gcattggaaa taaatattgg aattgccgat
     7261 tggaccggag atttaatttc cggattaagt gactcgctca ggtggaaaat gctgatatgc
     7321 gaatacggag gaatgaatga aacattggca aatacatacg cattcaccgg taataaaaag
     7381 tacctggaac tgtcaagaaa atttcaagac catcatatac ttgattcatt gggaatggaa
     7441 aaaaatgttc tcccgggaaa acattccaat acgcagattc ccaaagtaat tgggtccgcc
     7501 cgcagatatg aattaactgg taactcacag gatattgtaa caactttatt tttttggaat
     7561 gtgatggtga atatgcattc ttacgcaaac ggaggcaata gcaactatga aagattaagc
     7621 gctccgggca ggctcaatga tgaattgact gacaatacaa cagaaacctg caacacctat
     7681 aatatgctta aactgacaac gcatgttttt gctatctctc cgatgtcatg gcaaatggat
     7741 tactatgaaa gagccttata taaccacata cttgcttctc agaatccaaa caacggaatg
     7801 gtgtgctact ttgtaccact aagaatggga tcccggaaaa gatatagcaa tgaatttaat
     7861 aattttacct gctgccacgg atccggaatg gaaaatcatg tgaagtacgg tgaagcaatt
     7921 tacagtcatt caggagatgg aaaattattt attaacctgt ttataccttc caaattaagt
     7981 tggcatgaaa aaggagtgat aattcatcaa gacaccaaat ttcccgaaga agataaagtg
     8041 aggatcagga ttgaagccga gagatccata tcatttccca ttttaatccg ccggcctgag
     8101 tgggtaaccg ataactttag agtttcaata aatggcaagg aaataaagga atatgccggc
     8161 tccggtaacg gatatttgca tttggagcaa aaatggaaat ctggcgacga aattatcgta
     8221 gaattaccaa tgaaactata ttctgagccc atgccagaca atcccaatag ggttgccctc
     8281 ttctacggac cggtattgct ggcaggaaac ttcggagaga cagaaccgga tcctgaaata
     8341 ggtatcccat catttattac cggaaatcca gacgttgaaa catggttgga taagcgagat
     8401 gaaaaatcac tgcttttcag tactggaaag atgagcgata gcagcaaagt aaaccttatc
     8461 ccgttctatg aaaccattga cgaattttac agcgtctact gggatgtata tcatccaatt
     8521 cgatggaagg aagaactcag gagaatggag gacgagaaac aaagaaaact aaacattgag
     8581 aaccgtacga ttgatatgct gattattggg gataaggaat ctgaagaaag acagcgattc
     8641 gagggagttg aagtgagaac aggcgaaaat caccggaagt cgtttattcg tacaaatagg
     8701 aatggaagtt tttcttttat ttcgaaaatt aaacagggct cggaaaatac gcttcattgt
     8761 atttattggg gcgaagataa ccggggtcgc gcttttgata ttcttattga tggtgaactc
     8821 ctggcctccg ttgaacttgt aatatataag ggaagcaaat ttcatgagat cacttaccct
     8881 attccgccat cattaacaga gggaaagact gaagtaaaga tcgcttttca agcgaaggaa
     8941 aataatattg tggggccggt ttatggaatc aggatgttag gtaattaaac aatacctcct
     9001 cctaaatctt tcatatcctt aagactatga gctgatcaca ggaaagacgt ggtttcgaaa
     9061 tgtgaatttt gtactattct tacccaggga tagcatttcc ttccttatct gatttcctgt
     9121 cctgaacaat aaactttgta aatcaattag ggaattatgt aacaagcagc agaggctgta
     9181 ttgctaattt tgtctataaa tcaggagtaa aatcaagact aatgaataag aatcataata
     9241 aaattcagga gccagacaca ataaaatatt cctgccccat gaaatgtgag ggggataaga
     9301 cttacagcaa accagggaaa tgcccggtgt gcaatatgaa tttaattcct tttaacgaac
     9361 agaaatcaga agggaatact tgttgtcatc acgagcccgg ccaccaggtg catctccacg
     9421 acgatcatga tagtcattgc catatggagg aaaaaccagt gcaatccagt ggcaagaacg
     9481 agcaaaggga aggagccggg aaatactatt gtcctatgcg ttgtgaaggc gataagatgt
     9541 atgatgagcc tggcgattgt ccggtttgtg gaatgcattt acaacaggaa gtcaaacttt
     9601 catcaccaaa aagtaaaact cgtaaaacca cgtacacttg tccgatgcat ccggaagtta
     9661 agagtgataa gccaggcagt tgcccgaagt gcggaatgga tttagttcct gaaaagcctg
     9721 atgaggctgc agaaagtgat gaagaaaaat cgtataagaa gacactacgc aaattttggc
     9781 ttgcggtagc atttacgtcc cctgtatttg taatcgccat gtcagaaatg gtaaatatag
     9841 atctggaaaa tattgcttcc aggaaaatat ggggctggat tcagtttggt ttatccactc
     9901 ccgttatttt ttattcctcc tgggtatttt tcaagcgagg atggagttcc attagaaggc
     9961 gttcacctaa tatgtggacg ttgatttccc tcggtaccgg agctgcttac ttttttagta
    10021 taatagcttt aatagcgcca caaatttttc caccccaatt cagagacata caggggaatg
    10081 tacatttata ttttgaagcg gcaaccgtta ttcttacgct agtcttgttg ggacaggtga
    10141 tggaattgcg agcctatagc aaaacgaact cagctattaa gtcattactt aatcttgtac
    10201 cacccgttgc aagaaaaatg gttaatggca ctgaacacga aatcccgttg gccgaggtga
    10261 aggttggaga tactttaaga gttaagcccg gagaaaaaat cccggtggat gggacgatca
    10321 atgatggtaa tgcagtggtc gatgagagca tgatcaccgg agaatctatt cctgcagata
    10381 aaaccccggg agataaagtt accggcggaa caatcaacgg taaatcttct ttcgaaatga
    10441 aagctgataa agtgggaagt gaaactttgc tggcacaaat tattgagatg gtgaaccagg
    10501 ccggcaggtc acgggctcct atccaaaagg ttgcggatat tgttgcgaaa tattttgttc
    10561 agattgtaat cgctattgcc atcataactt ttgttgtctg gtcaattttc ggtccggagc
    10621 ctgcctacgt ctatgcattc gttaatacgg tttcagtgct aattattgct tgtccttgtg
    10681 ccctcggact cgcaacacca atgtctatta tggtggggac tggaagagga gctcaatccg
    10741 gaattctcgt taaagatgca acagcaattg aagaaatgaa taaaattaat gtgctggtaa
    10801 ttgacaaaac cggaaccatt acggaaggca agccaaggct aaaaaattac aaatcactgg
    10861 gagcttatga ggaaaaggca attctgaaat ttgcggcttc tcttgatgcc tacagtgaac
    10921 acccaatcgc ggatgcaatt gtaaaaggag cgaaggaagc taacttggaa ctggagaagc
    10981 tggaagagtt tgaatccgtt tcaggaaagg gcgtgcaagc catgttccgc ggcaaaagga
    11041 ttggcctggg gaataaaaga ctggttcaag attttaatgt tgccatcgat gaggaagtga
    11101 gtaaactggt tgaaggatgg caattggccg gagaaacagt catgtttctg atggtcgaaa
    11161 acaaaattga gggaataatc agtgtagcag ataaaattaa atccacatcc gcggctgcaa
    11221 ttaagtcatt gcaggagatg ggggtaaaag tatatatgtt gaccggtgac aatgaattta
    11281 cggccagagc ggttgcaaat gaaataaatc ttgatggcta tgagtcggaa tgtttgcctg
    11341 aagacaaaca taaaaaggtg aaaaacctgc aagatcaggg atttaaggtt gcaatggcag
    11401 gcgatggtat taatgacgcg ccagcgctgg cccaggcaaa tgtgggcatt gcaatgggca
    11461 caggtacgga tattgcaatg caaagtgcac aaattaccct ggtgaaagga gatttgactg
    11521 gaattgcacg ggcttatgag ctcagtaaaa aagtaatgcg aaatatcaag cagaatttat
    11581 tttttgcttt tgtttataac acgataggag tgccgcttgc tgccggcgta ttgtttccat
    11641 tctttggtgt cttgctgagc ccgatgatcg cggccgcggc tatgagtttt agttcggttt
    11701 cggtgattgg aaatgcgctg aggctgaaac agtgaaacag tgaagcagtg aaacagtgag
    11761 caggaagcag gaagcagtag ggttaatagt aattttttag taatttgcac tattcatata
    11821 aatattatac ctaatcaaat atcctaatag tgaagaataa aagtatcata atggaaaaat
    11881 ctttctcctt cgccgttaag gttgtggagt tagtaaaaat actatctaaa gaaaagaatg
    11941 aatatgttat gactaaacaa attctccgaa gtggaacttc tatcggggca aatgtttcag
    12001 aaggtttggg gggttcatca aaaaaagact ttgtctcaaa aatgtatatt gcttataaag
    12061 aggctaagga aacaatttat tggcttaatc tgttgcatgc aactaactac ctcaaccgtg
    12121 ataacttcaa ttcctttagg gatgattgtg aggaaattat taagatactg tattccattc
    12181 tgaaaacatc aagaaaaaat atcaattcag gaaactgatt ggctcactgc tcattgtttc
    12241 actgcctcac tgattcactg cctcactgcc tcactgtttc actgtttcac tgattcactg
    12301 cctcactgcc tcactgcttc actgcctaaa aggcttccga caagaaaaaa tataatccgg
    12361 tttgcctgct gccggggcct gcagaatctt tagataatcc ccagctatag tctatgcgta
    12421 tatttactct ggattccgga ttgaaaagta cacgcagacc tgttcctgcc gcataattga
    12481 atttatcaaa agcaaaattg ccactatatc catagacctg ggctcctgaa acaaacgcaa
    12541 ccatcccaag gcgtttccag attttgtaca gcggggcatc cacatcatca ctccagaagg
    12601 gaagccggta ttccgcttcg aaagaagcaa gattcttatc ctgaaaagta ccccggaagt
    12661 atccgcgcat gaagtcatga ccgccgaccc tggctaaacc gcgcatggga ataaccattt
    12721 catctgttct gctgaatatg aaattagcgc gcagtgcaat gctatgattt tttaccgggt
    12781 taaaatactt tcgtgcatca atgcgggtgt atgaataggt ataatcacta cctaacagct
    12841 taccgtagaa caaagcactg taatcaataa aaatcccatt cctggcattc agtatgttgt
    12901 ccctggaatc ataaaccagc gccaggccga ctccggaacg gaatccggtg ttattatttt
    12961 gcataagcga aatggcagat ccttcacgaa gaaaagtata ttgatcggga ataattttca
    13021 tagcgtattg atactccagg tcaaagcgca cgccgccata aattttatct ctcaatttca
    13081 ttaaaaagac aggcttgaaa gttattcgtt gaatggaaaa gtttagatag ttgagatcag
    13141 tgctatctgt cttgagtgcg ttatccaatt catattccct gataaaggcg ctggcattat
    13201 tacctaaacc gtagtttctg tcaggataca aattataacc ggcaaatcca cggaaccgga
    13261 atttgtcgcc gtcagtaaag atatcccaat tgctttcaac aatgatttga tttctggttg
    13321 tgtacacagc aacgaaatct atattagatt gccgggtagg atattttttt ccgagtcgca
    13381 aatagcggta cccgataatt cccagggtga ggccggtttc cggagcataa ctgattgccg
    13441 gcaacaaatc atatttccgg taacctttgg tggaatcttt ctcttgcgcg acagccgtta
    13501 ttgcaagtaa aacataaaaa agagaaagaa cagcaaattt acatttcata gccggagtat
    13561 gttaacacga atataatgag aaaaggaatt ataggggata agaattatcc tcaccattag
    13621 gaactttctt aacaaatttt ttacaccccc actagccctg gttccatggc aacagtcatc
    13681 taacggggta cctctaccca ctgtggtaag ttttttgtct gtcaaattta ctacttcaag
    13741 ttacgaccac cctgaactcc ctccctacca agggactgga aggggaaatt atgaataatc
    13801 cttttcccgc ttaaataata agccttcata ttctgattct gattgttaaa tttagattta
    13861 taatcaactg atcttgcgat atgaatacca caagaaccat ttgtacaatc ttttcattat
    13921 taatctccgt tcttctgatc aattgctccg gtgaaaggag caatcaggac gaacaatcag
    13981 aacagcctga tgtccaggca atgctggaag tcaaagaatg gcccgtaccc tgggaagata
    14041 gccgtccccg cgatccttat gttgccccgg atggaaaagt ctggttctgt ggccaggcag
    14101 gaaattatct tgcatccttt gatcctgcaa cggaggaatt caagcgattt gaagtaccgg
    14161 aacgtagcaa tccacataat ctgatcatcg acgatgaagg ctttgtgtgg tatgccggta
    14221 atttgaatgc gcatatcgga aaacttgatc ctgcagatgg atcaattaca cagtttccaa
    14281 tgcccgatcc ggaagcaagg gaccctcata ccctcgtgtt caaccaggaa ggcaacatct
    14341 ggtttactgt ccagggtgga aatttcatcg gttacctgaa taccacaaca ggcgaggtga
    14401 aactggtttc ggtccctact cccaaagcaa ggccatacgg catcaaaatg gatccgcaaa
    14461 atcaaccctg ggtggtgttg tttggaacga ataaactggc aacaatagat ccatcgacat
    14521 ttgagcttaa ggaaattgaa ctgcctgata gtaattcccg cccgcgcagg ttagagatca
    14581 aggaaggatt gatctattat gctgattact ccaaagggta tgtgggccgt tatgacacaa
    14641 acacaggaag ttttgcggaa tggcctctgc caggcggaag agattcaaga ccgtatggaa
    14701 cagccatgga tcaccaggga attatctgga tagcggaagc aggtcttgat cctaaccggt
    14761 tggttggatt cgatccggga aatggatcct tcgttggcat tacggaaatt ccgagcggag
    14821 ggggcgtcat ccggcacatg tatttttatc cccaaaagcg ggagatctgg tttggaacag
    14881 ataccaataa cctgggtcgg gcggtcgtta gtgcagcagg gagttaagaa agggaaatag
    14941 cagtattatt tgtccctgaa aggggaagcg cactttggct ctatgattca gcgcgtgtgt
    15001 gagtgaaaaa atgctgcaac cttttgaaaa gtattggaca ctctaacttt attgcgctgt
    15061 cattttctat tgcgctgtca ttttcttata catccacttc gtgctttcat ttttcatagt
    15121 ctggtgcttt gttaaaatgc tttcaatttc cttcaggttt tctcctgtta ccacataacg
    15181 ctttttgctg gcatttactt ttgaagtcat tatggcgtgt cctacgatac tgccaaccgc
    15241 cagcccggcg accaaattgc cggtaaccat tccaatggct gcaaaaaaca ggaattcacc
    15301 agtcggcgcg gtaatagcaa tgagggtggc gcccgcaacc aactgaccaa ttactccgta
    15361 accaaaccct ccccaaaact ctcccgactt gttttcatat atcgtaaggt gctcaatatc
    15421 tgataatgga aacgtctttt tagtccatct ccggaaaagt gtaacctttc cttcatttgc
    15481 agcaagcaca taaccagaaa accttttatt attatgcagc tttaccacag ctaaaaaaat
    15541 tggagaaaaa taaaacctca cattaagagc tttaagttgc ttatataaat ctctttgggt
    15601 aatgtctttg tcaacaaact caatataccg aattacatct ggagtaaaac taccatagtc
    15661 ggtaatgacc agtgaatttt caattctaat ggttttagtt ctcattacag aatcggagaa
    15721 cagaccaatt tgtgcaggct gtgctgaaac tgttgttcct gtaataagga aaatcatcac
    15781 cagacccgct ttaatacagc ctacaattat tttcttcatc ttgatttttt tggaaagtta
    15841 tccatttaat aacgcaggct gaaactgtat tttgcgcatt aatccacact ttcttgcacg
    15901 taatgcaaaa caaaaccaat gattaatgct ggattggtaa ttattgcaat taagtgtgac
    15961 agtattcgac ttgagtcaga tataattgca aatattcaca tggggtatac gcagaaagca
    16021 atttctttcc accaggtcgt ccgcctacct tcatccaaat aaaaataaat tcatgaaaaa
    16081 gataacaata cacctttgtt tactaattgg cgggagcctt ttgaccctga acttgttcgg
    16141 ccaggatcga tcaaacgatt ttcttttccc cgggaaaaat aaatttcagg caacgatctc
    16201 taccggaata ccctatgtcg ctttaattga gggaacgtat ggtataaccc gccggtttgc
    16261 tgcaggaatt atagtgggca ccacacctaa tgttatcgga tatggtataa gactccgcgc
    16321 ccaattgtac caacccaatg agaatttccg agtttatttc cggatgccgg ttttttatta
    16381 tgcgagcacc aaaaattttg gaaatgaacc ctggatgttg agctggccgg ttattgctgc
    16441 cgaatggaaa attgaagatt tagtaatgct ttcttttggg gtgggtgtag ttgaagccca
    16501 ttgtatcgat gcactattgg caggaatgaa tcaccatgac gagccaatag atatgggatt
    16561 tgaaggagga ttctggaata cggttaatgg cggtatcgcc tggccgttat ccaaaagttt
    16621 gacaatccag gcggagacat caatagtatt ggaaggttgg aaaattgcag gaagcaatta
    16681 cgtgggcggt cctccggtaa ttgtagtttt aggattgacg aaaaacttct gaaatggtta
    16741 gaataagttt ggtgcaatcc ataaatcagt aaactatgat ttcgccccac tggcggcgct
    16801 ggttttgatg gcaatgaaat cagaattgcc ggcactctga attcataata actccttaat
    16861 gagaagaagc tggattcatg cccgttacag aaggtgattt gataatacaa aatcatttcg
    16921 atctccacta gccgtggttt gtgtctcacg aaccacgaag caggggagtg gaaaacaaat
    16981 tactttcaca acgggcatga attttatttt acctttttga gattcatctc aataaatttg
    17041 ttccacgatg ttccgttatc cgctgagtac tcaccggtct ccagccaggt ttgcgcatcc
    17101 ggattataag tgataagata ttttactctt ccttcaaacc cccattcata ttgattttcg
    17161 ccaattatct ttaggtatgt gtctccctgc tggccggtat tcaggtaaga ctggaacctg
//
LOCUS       DASWHX010000068         4203 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_1159123, whole genome shotgun sequence.
ACCESSION   DASWHX010000068 DASWHX010000000
VERSION     DASWHX010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 4203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4203
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1159123"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            386..3466
                     /locus_tag="VGA21_04670"
     CDS             386..3466
                     /locus_tag="VGA21_04670"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEX9649829.1"
                     /translation="METPFNFAFALCVTALFLANSSIGQTTFNSVVNGNFASSTTWSP
                     VGNPGISDNVVINHLVTVSTAERVNDLTINNNSVNSASLSVSGTGTLTAYGNAFVNST
                     AGQSLAAEILIVAGATLSIAGNLEFDRDNEYNMACRLYMTGGNVGVGGDLIYHHRNSQ
                     SENQIEINMTGNSSLIISGNLELYQDPSIALSQIFSELKVEIQGTSTLSASNIVWQTN
                     RSSGGASDIRLIAIDDSNVDVINNVYLNQVNVSDEMVFGNAGNSILNIGGDLIIDKSA
                     GGSCWLVLTKATIENNILVTKTGGNHIYTDVSGEVMVKGTAGILYKASGGPSSSNTSE
                     FRIWPDGSLLIPNGSFRMESTNPATPGGSTELTINGNLDIGENLEFYSDNLNALLKIE
                     QSTANVTIGSDLIFDGVGTTTNGSGIFITNSTVNIGNDMNVTMNNSTNTRTMFFTLNG
                     AYDVSVGRDLVVNDFKGNLFFQSGTTAGSTGILSVGRNLDIKLLANTTTTTSLISSTF
                     EEATTLTVQGVTTLETGLAYANTNRNLFTFKESANFVSFGDIQLKSRGASNLGFEFSN
                     DAQLELQSTITRSSLPGYGYGRVLFMNQSSILLNGTNPQVIAQSNWPVPGTDKIQYNN
                     IILNNTSSIIPQFTLTGDVDVANTVNFLDGIVQTASTAKIIFSDNALASGYNSGSFID
                     GPVYKVGVSDFTYPVGDFNGTDAVLEPIRHASIFGGDANLIMAAEYTMLAAPNASNLD
                     ATLSGVSGIEFWDIGPVTAHTISADVSLYWTDALFSDITSLTLSPQDLTFAHFNLTQW
                     EDLGGAIDPGSTVGESGTGFITASMTSFSPVTFGTKGNVNPLPVTLIDFHGTVQGNST
                     MLTWVTASELNNHHYDLEKSNNGKDFSKILEVAGNGTTAEKHEYHATDQIGLGIYYYR
                     LRQVNFDNSSSYSSVIFVENSNLQANPTIIFPNPVSTNGKVSIGILPGYFEAGASLTI
                     LIISLQGDVVLETQINNSNGIMNLDLKDHPINTGLYLITVSDSLKRFQTKLVIE"
     gene            3569..>4203
                     /locus_tag="VGA21_04675"
     CDS             3569..>4203
                     /locus_tag="VGA21_04675"
                     /inference="COORDINATES: protein motif:HMM:NF014626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HEX9649830.1"
                     /translation="MCYDVRALTFKRGVYTNRFGVEFKDPRSADANIQNYHALGYDHP
                     YLPVISTNDPATIDFFQWGLIPHWVNDPKKVVAIQNSTLNARGEELMEKLSYRDAFQY
                     GHRCLILLDGFFEHHHKNGKIYPYYIALKTGEPFAVGGLWSYWKSIPGHERDTCTLIT
                     TQGNSLLAQIHNNPQLEGPRMPFIVPRELETTWLKEGLDPAEARLMISSFT"
BASE COUNT         1264 a          841 c          848 g         1250 t
ORIGIN      
        1 ccaaaatcct ttgggatttc ttgcaacttc cctgaagtgc cgggtctcaa gacttaaggc
       61 cacaccaaac atgataaagg caagagctag attcagcatc catacctttt cctgactgaa
      121 attcaggtga acagagttga ggtgatcttc caggtaaata agaaaattta tatcgaataa
      181 aaaattgctt gaaatttctg cttatccatc caaaaacaca acccttatct tttgagaaaa
      241 ttttataagt aactggaatt caaaggttat ggttcattta gattcactta tttatttaaa
      301 tattacattt aatatgtaaa aaactgttta aacataatat ttattattta ataagtaata
      361 tagcaatctt aaaagtgcca gtaatatgga aactcccttt aattttgctt tcgccttatg
      421 cgttaccgcc ttgttccttg caaattcctc aataggacaa accaccttca attctgttgt
      481 aaatggaaat ttcgccagtt ccacaacctg gagcccggta ggaaatcccg gaatttccga
      541 taatgtggtg attaaccatt tggtaacggt ttcaacagca gaaagagtaa atgatctgac
      601 cataaataac aattcagtaa actctgcttc cttatccgtt tccggaaccg gtactctaac
      661 tgcttatggg aacgcattcg ttaattcaac agctggccaa tcattagcgg ccgaaatact
      721 tattgttgcc ggagctactc tttccatagc gggaaatctg gagtttgacc gcgacaatga
      781 gtacaatatg gcatgccggc tttatatgac cggcggcaat gttggtgttg gcggtgacct
      841 tatttatcac cacagaaaca gtcaatcgga gaatcagatt gaaattaaca tgacaggtaa
      901 ctccagctta attatttctg gaaatttgga attgtatcag gacccatcca ttgctcttag
      961 tcagatattc agcgaattga aggtggaaat tcagggaact tctacgctct ccgccagcaa
     1021 tattgtatgg cagactaaca ggtcttcggg tggcgctagt gacatccggc tgattgccat
     1081 tgatgattct aatgtggatg tcatcaataa tgtgtattta aaccaagtca acgttagtga
     1141 tgaaatggtg tttggtaatg cgggtaattc cattctaaat attggaggcg atcttatcat
     1201 tgataaatcc gccggtggtt catgctggct tgttctaact aaagcaacaa tcgaaaataa
     1261 tattcttgtc actaaaaccg gaggcaatca tatttacaca gatgtctcag gggaagttat
     1321 ggttaaggga actgcaggaa ttctttataa ggcatcgggg gggccttcga gcagtaacac
     1381 ttctgaattc agaatatggc cggatggatc tcttctgatt cccaacggga gtttcagaat
     1441 ggaatccaca aatccagcca ctccaggcgg atccacagaa ctcaccataa atggaaatct
     1501 ggacattggg gaaaacctgg aattctattc cgacaactta aatgcgctcc ttaaaataga
     1561 acaatccacc gcgaatgtta cgattggctc ggatctgatt ttcgatggcg tgggcactac
     1621 aaccaatgga tcgggaattt tcattacaaa ttccaccgtg aatatcggta atgacatgaa
     1681 cgtaacaatg aataacagca ctaatacaag aacaatgttt tttaccctta atggtgcgta
     1741 tgatgtatcg gttggcagag accttgtggt gaacgatttt aaaggaaatt tattttttca
     1801 atcaggaaca actgctggca gcaccggaat tttatccgtt gggagaaatc tggatattaa
     1861 attattagcc aacactacca cgaccacttc attaataagt tccacctttg aagaagcaac
     1921 cactctaacg gttcagggag ttactacctt agaaaccggt ttggcctacg caaatacaaa
     1981 cagaaactta ttcacattta aagagagtgc taattttgtt tcctttggtg atattcaact
     2041 gaaaagtaga ggcgcgtcaa atcttggttt tgaatttagt aacgacgctc aactggaact
     2101 acaatccacc attacccgat cttcgctacc cggatacggc tatggaaggg ttctgtttat
     2161 gaatcagagt tctatcttgc taaatggcac taatccccag gtaattgctc aatctaactg
     2221 gcctgtcccg gggacagata agattcagta caataatatt attcttaaca atacaagctc
     2281 cataattcca caatttacac ttaccggaga tgttgatgtt gcaaacaccg tgaattttct
     2341 agacggaatt gttcagactg cttctaccgc caaaattatt ttttctgata acgcgttggc
     2401 cagtggctat aattcaggaa gctttattga tggtcctgtg tacaaggtgg gggtgagtga
     2461 ttttacttat cccgtgggtg acttcaatgg aaccgatgca gtacttgagc ctattagaca
     2521 tgcatcaatt tttggagggg atgcaaatct tattatggcg gcggaatata ctatgttagc
     2581 ggcacccaat gcttcaaatt tagacgcgac ccttagtggc gtttccggca ttgagttttg
     2641 ggacattgga cccgttacag ctcatacaat atctgcagac gtaagtcttt actggacaga
     2701 tgctttattc agtgacatca ccagccttac actctctccg caggatctaa cgttcgcgca
     2761 ttttaattta acgcagtggg aagatctcgg cggtgcgatt gatccgggaa gcactgtagg
     2821 agaatccgga accggcttca ttacagccag catgaccagc ttcagcccgg ttacatttgg
     2881 tacaaaagga aatgttaatc cactgcctgt tacattaata gatttccatg gaactgttca
     2941 aggcaattct acgatgctta catgggttac agcttcagaa ttgaataatc atcattacga
     3001 tttagagaaa tcaaacaatg gcaaggactt ttcaaaaatt ttggaagtag ctggaaatgg
     3061 tacaacggca gagaaacatg aatatcatgc aaccgatcaa attggcttag gaatatatta
     3121 ctatagactt cgccaggtta atttcgataa ttcatcttcc tattcaagtg tcatttttgt
     3181 tgaaaactcc aatcttcagg caaatccaac aataattttt cctaaccctg tttcgactaa
     3241 tggtaaagtt tcgatcggaa tactgccagg ttattttgag gctggggcct cccttacaat
     3301 tttgattatt tcattgcagg gtgatgttgt tctcgaaacg caaatcaata actccaatgg
     3361 aattatgaat ctggatttga aagatcaccc cattaatacc ggcctgtatc taattacagt
     3421 aagtgattca ttaaaaaggt tccagacaaa gcttgtaatt gaataaattc gccggaaagc
     3481 aaattttcct tcgccatcct tctaaattgt aaaatcaatc tggtttttac agattttttg
     3541 ttaattatgt cgttgcaagc aggttattat gtgttacgat gtaagagctc tcacattcaa
     3601 acgtggtgta tatactaaca ggtttggagt tgaatttaaa gatccgcgtt ccgccgatgc
     3661 aaatattcaa aactaccatg cccttggata tgaccatcct tatcttcctg tgatttcaac
     3721 taatgatccc gcaactattg atttttttca gtggggacta atacctcatt gggttaatga
     3781 tccaaagaaa gttgttgcca tacaaaactc gacactaaat gcccgtggtg aagagttgat
     3841 ggaaaaacta tcctaccgtg atgcttttca atatggtcac cggtgtttga ttttgctgga
     3901 tggatttttt gaacatcacc acaaaaatgg aaaaatttat ccttattata ttgctctgaa
     3961 aacaggagaa ccatttgccg taggtgggct atggagttac tggaaatcca ttccaggcca
     4021 tgaacgagat acgtgtacgc ttatcaccac gcaaggcaat tcattattgg cccagattca
     4081 taataacccg cagttggaag gtccacgcat gccatttatt gttccgaggg aactggaaac
     4141 gacatggctt aaagaaggct tggatccggc tgaggcaaga ctaatgatta gtagttttac
     4201 cca
//
LOCUS       DASWHX010000069         6731 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_3012786, whole genome shotgun sequence.
ACCESSION   DASWHX010000069 DASWHX010000000
VERSION     DASWHX010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 6731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6731
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3012786"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(61..2469)
                     /locus_tag="VGA21_04680"
     CDS             complement(61..2469)
                     /locus_tag="VGA21_04680"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9649831.1"
                     /translation="MLTNYLKTAYRNLLKNKSYSFINILGLSVGAAASMLIFLYVRYE
                     YNYDTFFKDNDRIYRVVIERRYPDHVSHFALIPGGFSTIFADELPEIENSVRLVGFPN
                     FASVIRQEEIINQEYYLFSADSNFFEIFDFKLLQGNPATALSKPGTVVTTQSTAKKYF
                     AEEDPIGKFLEIDAGNFEVVGVMEDIPAQSHLKLDLLTSSENVGFLRDPSFYISGTYT
                     YIKLRPGADPGAVEAKIPALVEKYAAGQIERNLGLSYKEYTDAGNGYIYTLQPVRDIH
                     LHSNLGNEIKANGNITYVRMLLFISILILIIAGINFVNLATARSAERAKEVGIRKVMG
                     SLRKQLIFQFLSESFLLSFVSLIVAIFIIQAFLSYFNNLTQRPLEFNFITGPETILIL
                     GITIFFGILAGIYPAFYISALKPVQVMSGKFKNSAKGNTLRKGLIIFQFTISIILISS
                     SILMFNQFRFIQSKDLGFDKTNLMILNQNGTAGDADALKNELEKVPGVQSVATTNANP
                     GGYFFGLQFRKPGDSEIYTTKGLPVDDHYISTMGIEVLEGRDFSPETNDSLNVILNES
                     AVKTLGYEDPIGARLINSLDTVNRIYTIIGVVKDFNFESLRNEIIPMVIVNLEGEFGF
                     FSQLSIRIAGESTNEIIPEVESIYKELNPQNPFIYTFLDETLDRQYSSEYTSGKMLGF
                     FTIIAIVIACVGLFGLAAYSISQRTKEIGVRKVLGASVRSITLLIFGDFGRLIIAAFI
                     IATPVSYYAMDRWLESFAYKTSLNLWVFLLAGILVLFITTITISYQSISAATVNPAKS
                     LKSE"
     gene            complement(2645..3079)
                     /locus_tag="VGA21_04685"
     CDS             complement(2645..3079)
                     /locus_tag="VGA21_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020595583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649832.1"
                     /translation="MEKHFGLTDQQFEYQFETTTLAPSLFSHEAHLRLAWIHIKKYGV
                     EKAIDNVSAQIMAFANHHGDSKKFNVTVTVAAVRAVYHFILKSQSSHFKDFIKENPRL
                     KTSFKDLIKSHYSGDIFSSHLAKEIFLEPDLLPFDEIPSKGK"
     gene            complement(3106..3849)
                     /locus_tag="VGA21_04690"
     CDS             complement(3106..3849)
                     /locus_tag="VGA21_04690"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEX9649833.1"
                     /translation="MKEHRDNTKFGVLLETNKLARFAEIILVFISAFTLIKLLEPLAG
                     DNLVLKQAIIWLANILMLILVWLGIKLRGNKLSDFGITFKPASLKESLNVFYLSLLVF
                     ITAVVAFILGSIIMANITGIPETANLSNYEYLQGNIFMLLLTLAGVYIVSSFGEEVIY
                     RAFLINRFIELGLDGKTGRFLAVFFSAVIFGLVHYEWGPMGIVQTGFMGLALGLFYLK
                     LKKRIWILILAHAYMDTILMVQMYLANSE"
     gene            complement(3939..4310)
                     /locus_tag="VGA21_04695"
     CDS             complement(3939..4310)
                     /locus_tag="VGA21_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015814787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEX9649834.1"
                     /translation="MKMIKAKDVDAYIAGFPNNTQKLLKQIRATVKKAAPKAVEAISY
                     GMPVLKLNGNLVYFAAYKFHIGFYPSSSGIRTFEKEISKYKWSKGAVQFPIDKPMPLS
                     LITKIVKFRVRENLAKGAKKK"
     gene            complement(4420..4902)
                     /locus_tag="VGA21_04700"
     CDS             complement(4420..4902)
                     /locus_tag="VGA21_04700"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX9649835.1"
                     /translation="MNSISDYYKKQYELVLESRKVLLDYCKTVSAPDFLNANSSFGRG
                     GSIRNLLVHIANTYEFWIANNALKKNIDYTKYESVNSVEEAIALFRQVDSGMFEFIEH
                     VEKDQTQQIEYEVNGKKRAVEPFNLFSHVITHEFHHKGQILSLSRHLGYTPVDTDIIR
                     "
     gene            5316..6119
                     /locus_tag="VGA21_04705"
     CDS             5316..6119
                     /locus_tag="VGA21_04705"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019600517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HEX9649836.1"
                     /translation="MKFNQNKIRFALRFLTGILFVMILTSGRCSNQNNKEEKENANSG
                     IEVKFSQTDWRKEAKEMVDHQIRSRNVSNKSVLKVMEETPRHNFVPDSYRRYAYTDQP
                     LPIGEGQTISQPYIVALMTELLELQGDEKVLEIGTGSGYQAAILSQLTREVYTIEIVE
                     PLYELSTAILEQMGFDNVKVKLGDGYQGWIEHAPFDAIIITAAPPEIPPKLIEQLKPG
                     GKMVLPVGTVTQELKVVTKTLDGKIMERTEIGVRFVPMVHPKDSLKKNR"
     gene            6129..>6731
                     /locus_tag="VGA21_04710"
     CDS             6129..>6731
                     /locus_tag="VGA21_04710"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEX9649837.1"
                     /translation="MAAIKEVSENPGLRKLGWISAISIVVANMIGTGIFTTTGFLAAD
                     LGAPVPILIGWVLGGIIALCGALTYSELAVNIPGNGGEYLYLSKLYHPALGFLSGWVS
                     LFAGFSAPIAAASVAFGHYAHAIFPLADPVLSSVVLLIVFTLLHIVNVQAGGTIQSII
                     TIVKVLVIILLVVAALLIPDKPTFIQAGENDFQVVISPAFA"
BASE COUNT         2013 a         1329 c         1337 g         2052 t
ORIGIN      
        1 attgaacaat agccaccggt tctttcaaaa tacatctgtc ttccgtccaa aatcaacttt
       61 ctattctgac ttgagacttt ttgccggatt cactgtggct gcgctaatag actggtatga
      121 aatggtaatt gtcgtaataa acaacaccaa tattcctgca agtaaaaaca cccagagatt
      181 aagagaggtt ttataggcga aactttcaag ccagcggtcc atggcataat aggaaacagg
      241 agtcgcgata atgaatgcgg ctatgattaa ccgcccaaaa tcgccgaata ttaaaagcgt
      301 tatgctcctc acgcttgcgc ccaaaacttt tcggacacca atctccttgg tgcgctgact
      361 gatggagtaa gccgctaacc cgaatagtcc tacgcaggca atcactatcg cgattattgt
      421 aaagaagccc agcatcttac ccgaagtata ttctgaagaa tattggcgat ccagcgtttc
      481 atccagaaat gtgtaaatga atggattttg cgggttcaat tctttataaa tgctttcaac
      541 ctccggaatg atttcgtttg tactttcacc ggctatcctt atagaaagct ggctaaaaaa
      601 tccgaactct ccttcaaggt ttacaataac catcgggata atctcatttc tcaatgattc
      661 gaaattgaaa tctttaacca cacctatgat cgtataaatg cgattgacag tatccagtga
      721 attgattaac ctggcgccga tgggatcttc gtaacccagg gtttttacag cagactcatt
      781 caggattaca ttcagcgagt cattggtttc ggggctaaaa tcgcgaccct caagaacttc
      841 tattcccatc gttgaaatgt aatgatcatc aacaggaagg cctttggtag tataaatttc
      901 agaatccccg ggtttccgga attgcagccc gaagaaatat ccgcccggat tagcatttgt
      961 agttgcaaca ctttgaactc ccggcacttt ctcaagttca tttttcaaag catccgcgtc
     1021 tccggcagtt ccattctgat ttaaaatcat gaggtttgtt ttgtcaaatc caagatcttt
     1081 tgattgaata aaccggaatt gattaaacat taaaatggat gatgaaatca ggataatgga
     1141 gatcgtaaat tgaaaaataa ttaatccttt gcggagcgta ttgccctttg ctgaattctt
     1201 gaattttcca ctcatcacct gaacgggctt aagcgctgaa atatagaaag cgggataaat
     1261 gccggccaat atgccaaaga atattgtgat tcccaggatg agaattgttt cagggccggt
     1321 aatgaaatta aattcgagag gtcgctgtgt taaattattg aagtagctta agaaggcctg
     1381 aataataaaa attgcaacta ttaagctcac gaagcttaac aggaatgatt cagacaggaa
     1441 ttggaatatc aattgcttgc ggagggaacc catcactttt cttattccaa cttctttggc
     1501 gcgttccgcg gatctggcgg tggccaggtt aacgaaatta attccggcta ttattaaaat
     1561 gagaatgctg atgaaaagca gcattcgtac atacgtaata ttaccattcg ctttgatttc
     1621 atttcccaaa tttgaatgca agtgaatatc ccgcaccggc tgaagggtat aaatgtaacc
     1681 attacctgca tctgtatatt ctttgtagct gagaccgaga tttctttcga tttggccggc
     1741 ggcatacttt tcaaccagag cagggatttt ggcttccact gccccagggt ctgccccggg
     1801 tcgtagtttt atgtaagtgt aggtaccgct aatataaaag cttgggtccc gaagaaatcc
     1861 aacattctcc gaagaagtaa gcaaatccag cttaaggtgg gattgtgctg gaatgtcttc
     1921 cattacacct acaacctcaa agttaccggc atcaatctcc aggaatttac ctatggggtc
     1981 ctcttcagcg aaatatttct tcgcggtgct ttgtgtggta actacagttc cgggtttgct
     2041 gagcgcggta gcaggattac cctgaagtag tttgaaatca aagatttcaa aaaaattaga
     2101 gtcagcggag aaaaggtaat attcctgatt gatgatctct tcctgcctta taacggaagc
     2161 aaaatttgga aatccgacga ggcggactga gttttcaatt tccggcaatt catcagcaaa
     2221 gatggtgctg aagccgcctg gaatcagggc gaaatgcgaa acatgatccg gataccttcg
     2281 ctcaatcacc acacgataaa tgcgatcatt atctttaaaa aaagtgtcat agttatattc
     2341 ataccgcaca tagaggaaaa tcaacattga ggctgcagcg ccaactgaaa gccccagaat
     2401 attgataaag gagtaactct tatttttcaa aagatttcga taagcagtct tcaggtaatt
     2461 tgtcagcatg atgtcttcgg gttaggttaa agtccaaatt tccaacaact atgccagttt
     2521 ctggaatacg aaagaaggca aaataacctg gttattaaga agccaattgt tcgggatgaa
     2581 acaataatgt tcggttttga cacaaatcct gaatcgcaaa cttcatgatt tgaaaacaat
     2641 ttattcactt tccttttgat ggtatttcat caaagggaag caagtcgggc tcaagaaaga
     2701 tctccttggc tagatgcgaa gaaaatatat ctcctgaata atgagatttg attaaatctt
     2761 tgaaactggt tttcaggcga ggattttctt tgataaaatc tttaaagtgg ctggattggg
     2821 atttgagaat aaaatgatac accgcacgga ctgccgctac tgttacagta acattaaatt
     2881 ttttgctatc accatgatga tttgcaaaag ccattatttg tgcggaaaca ttatcaatgg
     2941 ctttttccac accatacttt ttaatatgaa tccaggccag gcgcagatgc gcttcatgac
     3001 tgaacaggga tggagccagt gtggtggttt caaactgata ttcaaattgc tgatcggtga
     3061 gcccgaagtg tttttccata ttcaacattt gtttatcagc cacggttatt ctgaattggc
     3121 caaatacatt tgcaccatta aaattgtatc catatatgca tgagccagga ttaagatcca
     3181 aattcttttt ttcagtttta ggtaaaacag gccaagagcg aggcccataa atcctgtttg
     3241 tacaattccc attggtcccc actcatagtg tactaatccg aaaataaccg cgctgaaaaa
     3301 taccgccaga aatcgtccgg tcttgccatc caatcccagc tcaataaaac ggttaattaa
     3361 aaatgcccgg taaataactt cttcaccgaa ggaggaaaca atatatacac ctgccagggt
     3421 cagcaataac ataaaaatat tcccttgcaa atattcataa ttgctcaagt ttgctgtttc
     3481 cggaatacca gtaatgttgg ccattataat agatcccaaa atgaaagcaa ccacagcggt
     3541 aatgaatacg agtaaggaaa ggtaaaatac gttcaggctc tctttaaggc tagcaggttt
     3601 aaatgtaatt ccaaaatcac ttaatttatt tccgcggagt tttataccca gccaaacaag
     3661 aatcaacatc agaatattcg ccagccagat gattgcttgc tttaaaacca aattgtcacc
     3721 ggctaatggt tcaagcagtt tgatcaatgt aaaagcggaa ataaatacca aaataatttc
     3781 tgcaaacctg gctaacttat tcgtttccaa aaggacgccg aactttgtat tatccctgtg
     3841 ttctttcatt cagactgttg ttttttttaa gtcttaatca ggcgtttcaa agattttggt
     3901 aattatattt aattattctc ttttcgacat tcctaaatct atttcttttt agcgcccttt
     3961 gccaggtttt ctctcaccct gaactttact attttagtaa ttaatgataa tggcattggc
     4021 ttatcaattg gaaactgcac ggctcctttt gaccatttat attttgaaat ctctttttcg
     4081 aaagtcctga taccggaaga tgatggataa aatccaatgt gaaatttata agcggcaaaa
     4141 taaaccaggt taccatttaa tttaaggaca ggcattccat aactaattgc ttccactgct
     4201 tttggcgccg ccttttttac ggtagcccgt atttgtttca ataatttctg ggtattgttt
     4261 ggaaaaccgg caatgtaggc gtcaacatct ttagctttga tcattttcat gggttggtgt
     4321 tttgattttc agaattttat caggctaaaa taaggatagc attaatattt agctaagggt
     4381 ttaccaagca aaatcaaggt gcattttaga cataaaagtt catctgatta tgtcagtatc
     4441 tacaggtgta tatcccaagt ggcggcttag agataaaatt tgtcctttat gatgaaattc
     4501 gtgagtaata acatgactga acagattaaa aggttcgaca gctcttttct ttccgttcac
     4561 ctcatactca atttgctgtg tctggtcttt ttccacatgt tcaataaatt caaacatacc
     4621 actatcaacc tgacggaaca atgcaattgc ctcctcaaca ctattaactg attcatattt
     4681 tgtgtaatca atattctttt tcaatgcatt attagcaatc caaaattcgt acgtgtttgc
     4741 gatatgaacc agtaggtttc tgatacttcc acctcttccg aaagagctgt ttgcattcaa
     4801 aaagtctggt gcagaaactg tcttgcaata atcaagtaaa acctttcttg attcaagaac
     4861 cagttcgtat tgttttttat agtagtctga aattgagttc attttaatgt cagctgaaca
     4921 ttaaataatt ttcccggcaa tttagggttt tccacaataa tttcactaag agttctctta
     4981 ttgtattatg attattcaag atcggaaaaa aaagaatgcc ggtaccgggc aaatacgtac
     5041 atacaaattt tttagcgccg gagtttctta gtttttaagt tatttcaaaa ccttatagga
     5101 accattcacc ttatcaacaa acatcatcgg gcatccatta ataataactt ttatgtgatt
     5161 ttcttcggct gttttttccg cttttttagt gaaactttcc ctggccaatt cctttatgca
     5221 taagagaaat tcaagaattt attagcctgc tattcatatt aatattagat tgaattaacg
     5281 gcaatcattt cttagtataa actatatcca gagctatgaa atttaatcaa aacaaaatca
     5341 gatttgctct gagatttctt accggaatcc tgtttgtaat gatcctcact tccggacgct
     5401 gttctaatca aaataataaa gaagaaaagg aaaatgcgaa ctcaggaatt gaggtgaaat
     5461 tttctcagac ggattggaga aaagaggcaa aggaaatggt ggatcatcaa attcgttcca
     5521 ggaatgttag taataaaagt gtattaaagg tgatggagga aactccacgt cacaattttg
     5581 ttcctgattc ctaccgtcgc tatgcgtata ctgatcagcc cttgccaatt ggtgagggac
     5641 aaaccatttc acaaccttat attgtggcat tgatgaccga attgctggaa ttgcagggag
     5701 atgagaaggt tttggaaatt ggcaccgggt cgggatatca ggctgcaatt ctctcccaac
     5761 ttaccagaga agtttacaca atcgaaatcg tggagccatt gtatgagcta tctactgcta
     5821 ttttggagca aatgggattt gataacgtaa aggtaaaact tggagatgga taccagggat
     5881 ggattgaaca tgcgccgttt gatgcgataa ttattacggc agctccacct gaaatcccgc
     5941 caaaactgat agaacaatta aagccgggcg gaaaaatggt tttaccggtg ggtacagtta
     6001 cccaggaatt gaaagtggtt accaaaacat tggatggtaa gatcatggaa aggacagaaa
     6061 taggagttag gttcgtgccg atggtgcacc ctaaagacag cctgaaaaag aaccgctaaa
     6121 gctggattgt ggctgcaatt aaagaagttt cagaaaaccc cggtctcaga aagcttggat
     6181 ggatatctgc aatctctatt gtagttgcca acatgatcgg gactggtatt tttacgacta
     6241 ccggatttct tgctgcagat cttggcgctc cggttccaat acttataggg tgggtgttgg
     6301 gcggaataat tgcattgtgc ggcgcattaa cctactcgga actggcggtt aatattccag
     6361 gcaatggagg cgaatatctg tatctttcca agctgtatca tcccgcgttg ggatttctct
     6421 ccggctgggt gtctctcttc gcgggctttt cggcaccaat tgctgccgct tctgtcgcct
     6481 ttggccatta tgctcatgca atctttcctt tagccgatcc ggtactaagt tcggtggtgc
     6541 tgctaattgt atttacgctt ttgcatatcg ttaatgttca ggccggtgga accattcaaa
     6601 gtataattac tattgtaaag gttctggtaa ttattcttct tgttgttgcg gccctcctta
     6661 tacctgataa accaaccttt atacaagccg gcgaaaatga ttttcaggtt gtcattagtc
     6721 cggcgttcgc g
//
LOCUS       DASWHX010000070        25446 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_2275390, whole genome shotgun sequence.
ACCESSION   DASWHX010000070 DASWHX010000000
VERSION     DASWHX010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 25446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25446
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2275390"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..476)
                     /locus_tag="VGA21_04715"
     CDS             complement(<1..476)
                     /locus_tag="VGA21_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017733417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4442 domain-containing protein"
                     /protein_id="HEX9649838.1"
                     /translation="MFDIQKIIEKAKTSTFYLWLLNFGLNRMVPFNRPHGFKIVKLTD
                     ESIETKLPFKTLNFNHIRSVHACALATLTEFTTGVFLISKFGANKYRIILRNLQMEYH
                     YQGKTSVIAKFGLSEKEMRERVFIPLENEESVDIICEVKTLDLNNNHISTGSVTWQ"
     gene            500..1645
                     /locus_tag="VGA21_04720"
     CDS             500..1645
                     /locus_tag="VGA21_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020531796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HEX9649839.1"
                     /translation="MQANELKIKVMVLGCGLVGKTIAGDLRKNFEVTVVDFNTEILKE
                     MNSNGFRTLQADLSDSNTIKEIIKDHELVVGAVPGFIGYNLVKAVIEAGKNIVDISFF
                     PEDQFTLEQLAIDKGVTAILDCGLAPGMGNIILGYHNANMEVHEYECLVGGLPVEREW
                     PYEYKAVFSPGDVIEEYVRPARFVKNGGIVIREALSDPELVNFEKVGTLEAWNSDGLR
                     SLLKTMKIPNMIEKTLRYPGTIEYLRVLRDTGFFSSEQIEVKGIMIRPVDLTSKLLFP
                     KWKLKPGEKEFTVMRIIISGIENGDRVKYTYSLYDEYDPEAKQTSMARTTGFTCNAAV
                     NLLARKKFDQKGLILPEYLGTDRDNFDFILNYLKERNVDYQMKRGVE"
     gene            1712..3175
                     /gene="nhaC"
                     /locus_tag="VGA21_04725"
     CDS             1712..3175
                     /gene="nhaC"
                     /locus_tag="VGA21_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015266608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HEX9649840.1"
                     /translation="MTNSKAQPSLMIALVPVIALIAMLAASVIVFGDSAIEGANQMSL
                     LMAALVCGILGIGLGYKWDELQKGIVKSISSAMSAILILLFIGSLAGTWLLSGIVPAM
                     IYYGLQILNPAIFLFAACLVSAIVSLATGSSWSTIATLGIALLGIGKAMGMSEGIIAG
                     AIISGAYFGDKMSPLSDTTNLAPAMAGTDLFTHIRYMVYTTGPTMTITLIIFLVMGLT
                     IDTSMTDASMNEIMASIDGAFNINGWLFLVPLLVIVLIVKKVPALPALIGGTLLGGVF
                     AIIFQPQAIDYVSGIPGAGITSAFVAVWKSMYGAISISTDNEMVNELLSTGGMSGMLN
                     TIWLILCAMIFGGVMEACGMLQRIAAFIVQGAKSTGSLIAATTGSCIFFNITASDQYL
                     AIVVPGRMFRGAFKERGLKPEVLSRTLEDSGTVTSVLVPWNTCGATQSTILGVSTFAY
                     APYCFFNIISPLMTILFGYLNIKIRRFVTEGEVKKED"
     gene            3178..3768
                     /locus_tag="VGA21_04730"
     CDS             3178..3768
                     /locus_tag="VGA21_04730"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEX9649841.1"
                     /translation="MFKPEITREELNELPLGSFNGDIHVITEAGQIKSIVQKLRKLNF
                     VGFDTETRPAFVKGQKFPISLTQLACDDEVFLIRNKYTGWPDSLAHFLADPDVLKIGV
                     GIRDDIKSIQSEIRSNWSPEGFVELNHIVKELGIVSQGIRKLSGLVLGFRVSKSAQTS
                     NWERENLDEKQLRYAATDAWCCHRIYSELLARGYLN"
     gene            complement(3742..4362)
                     /locus_tag="VGA21_04735"
     CDS             complement(3742..4362)
                     /locus_tag="VGA21_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009582592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HEX9649842.1"
                     /translation="MKENFFTIKEKSKGLFKDKGSKFLAFAFPVQSADQVRSHLQELK
                     SKYHDARHHCYAYVLGPQGQEFRSNDDGEPNHTAGDIILGAIRSKNLTNVLVIVVRYF
                     GGTKLGKRGLINAYRFSSEAALDGSIIIEVEILIEISITFPYSGTSEVMYQLNKAGVI
                     IYEQEYKEICTIRFGVKIKNKQELADKLRRLQEKKIIHSIEITSGQ"
     gene            complement(4363..5253)
                     /locus_tag="VGA21_04740"
     CDS             complement(4363..5253)
                     /locus_tag="VGA21_04740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649843.1"
                     /translation="MISLLLVVILNVFILILFRLLSQFLVNTFQTIVINYWTCVVIGL
                     IISGGIQIATPEGLKSWIWFALLLGGIFVITFFLIARTSQVFDVSIASLSSKVSLAIP
                     VFFSLVIFQSPLRNFDWLNYLGLFLTFVAIITSGLKKELNVVIPRNKHFVLLPISVFF
                     LSGVIDTSLNYATLKLISSEESLDFTIVVFFSAALLGTIAFVFGKKKFESGSIPWGVT
                     LGVINVFTIVLLLSTLRHFNNDGAFVFPLVNIGIIIFATVASGFIFKEKWNKFKVIGI
                     GFGILALLLIAHQEIFQFLR"
     gene            5333..6250
                     /locus_tag="VGA21_04745"
     CDS             5333..6250
                     /locus_tag="VGA21_04745"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX9649844.1"
                     /translation="MGYQFQQLSEPINVPDFSLFNSIDFLSRHQSNLLQFLIKRKSEI
                     VGVIHFNLIGDKALSPWKASFGGFEFSKELPYSIISHFVTWVISQVVRKNIAEIQITF
                     PPDFYKNSAYEIMSDILQDCDFQVCVETGQALVCGEKNFSQLINRFERSKLERSLSDS
                     FRSWKGIGELPEAAFQMIVKNRERKNIPVKLEYAELKENFVKFSDNFHWFGVWKGDEI
                     AAASVGIRVQPEIMYHFFPGHDNKFDAWSPMVLLLNELYGYCLKKEIKYLDLGISSVD
                     GKINQGLYQFKKNLGAMEYKQAKFKWTAE"
     gene            6235..7161
                     /locus_tag="VGA21_04750"
     CDS             6235..7161
                     /locus_tag="VGA21_04750"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX9649845.1"
                     /translation="MDSGIVNFPKVSVICTSYNHQDYVLEALNSVMSQTYPNIELIVV
                     DDNSSDKSANRILQFQARNPETLVILNDKTLGICKSFNKGFRNSSGAYLIDLAADDIL
                     HNDRIEKGVQSLENLGKPFGVNFCDCEIIDQASETLGYQYKGKINYNKVPQGEVFASL
                     LSRHWISSPTLLFKREVIEKLRGYDETLSFEDFDFWVRSSRYYHYSFTPEVLVTKRKL
                     HDSLSSVQYQLHNSHIRSIIYVCKKAVVLVREKRERKALQKRIVIEMRRAIKTHNIRE
                     AKAFLQLLRSTGSFFRFWGYHFLVSSFRSMLR"
     gene            complement(7122..8042)
                     /gene="rsgA"
                     /locus_tag="VGA21_04755"
     CDS             complement(7122..8042)
                     /gene="rsgA"
                     /locus_tag="VGA21_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009581130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEX9649846.1"
                     /translation="MIGKVMKSTGSWYLVSYDGAIWQCRTRGKLRLEDSTATNPISVG
                     DEVEFEKETGSASNGVITNILPRRNFITRKASRKTQESQVIAANIDIAALMVTLFSPR
                     TSPGFIDRFTVSAESFRIPVFLIFNKIDLHDTKSREEQARLKDMYSLIGFKCFEISAK
                     EGTGLDKLAENLTGKVILLSGHSGVGKSTLLNVWSENIAQKTADISSFSTKGVHTTTF
                     AEMFEVFPQTFIIDTPGIKEFGLIDIEEFELSDYFPEMRAMRGKCKFHNCTHYHEPKC
                     AIKAAVEEGTIFESRYRSYLSMLRNEDTRK"
     gene            complement(8039..9274)
                     /locus_tag="VGA21_04760"
     CDS             complement(8039..9274)
                     /locus_tag="VGA21_04760"
                     /inference="COORDINATES: protein motif:HMM:NF016311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HEX9649847.1"
                     /translation="MGNFFYSIFLFSYKLFLVLTSPFVEKAKLMLTGRKNWRAAIKQK
                     FQENKEPVVWFHAASLGEFEQARPLIELFNRNFPQYKILISFFSPSGYEIRKNYQPSF
                     YICYLPFDSRKNASFFLDTINPSLVFFVKYEFWYYYSSEIKKRKLPMLSFSAIFRKSQ
                     VYFQWYGGFNRKILNNFTYFFVQNQESADLLNSIGIKNVELAGDTRFDRVLEIASQRK
                     EIQQIDRFKKNKKLFVCGSIWESDLEVINELIARLKESYTFILAPHDISLQNLNAIKE
                     KLNYPCNTHSDLENEDGNFILLLNTMGMLASVYGYAEAAYIGGAFGKGLHNILEASVY
                     KIPVFFGNRNYKKFNEAVELANLGAAFPVSGGNEIADRLNGLEGKEESLEVIIRQYFE
                     SKRGATERIFYFSKKLLEA"
     gene            9380..9889
                     /gene="tpx"
                     /locus_tag="VGA21_04765"
     CDS             9380..9889
                     /gene="tpx"
                     /locus_tag="VGA21_04765"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011586877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HEX9649848.1"
                     /translation="MTQVTFKGSPVELPGNIPSVNTIASDFAYVKRDLSESRLSKLLG
                     NVIVLMAVPSLDTGTCAKETKKFNEKLANLTGVLGIVISKDLPFALRRFCEVEGIQNV
                     DAVSDFRYNEMGKNYNIEMLDGPMKGLMARAVWVIDRDGKIVYSELMKEVVLEPDYDK
                     VLETIHSLL"
     gene            10093..11121
                     /locus_tag="VGA21_04770"
     CDS             10093..11121
                     /locus_tag="VGA21_04770"
                     /inference="COORDINATES: protein motif:HMM:NF042644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6588 family protein"
                     /protein_id="HEX9649849.1"
                     /translation="MKAKVKRNLVVVILTILFIVPNQKGFAQLDQLDKLLQAGEEDAN
                     ALLSPYIAPFMRGFGYGLANSWYNTAEVHKGFGFDLMITGTLAYVPGEDLKYNVLDLN
                     LQEIEIVSPRDGEVPTVFGDDLITPQYQFKSTPGLTFDGPTGLNLQDEIGGKYIPTPM
                     VQIGIGFPLGITIRGRYMPEVDAGQFKSSFWGVGIQHDIKQYLGAVDKIPFDLSVLVG
                     YTQLNSTIGLSGAIDDGGAGDQEGVIKANAWTYQVLISKNLSIVTFYGGVGANTLRSR
                     VQILGTYILTDPGIITLTDPINQKYKDGGVRGTLGMKLKLGPITLHGDYTFQKYNALT
                     AGIGVSVR"
     gene            complement(11209..11982)
                     /locus_tag="VGA21_04775"
     CDS             complement(11209..11982)
                     /locus_tag="VGA21_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013455830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX9649850.1"
                     /translation="MEFLNVQSTYAPMVALVQINRPKEMNALNWQLMAELRDTLKSLD
                     ANDEVRVIVITGNEKAFAAGADIKQMADKSAVDMLKIDQFSTWDQIRKTRKPMIAAVS
                     GFALGGGCELAMTCDMIVASETARFGQPEIKIGVMPGAGGTQRLTKAIGKAKSMELIL
                     TGKFLSAEEALDYKLVNKVVPVEMYLEEAVSLAKEIAEMSPVAAMLAKEAVNRSFEAH
                     LDEGLNFERKNFYLAFSSEDQKEGMSAFIEKRKPEFKGR"
     gene            12127..12597
                     /locus_tag="VGA21_04780"
     CDS             12127..12597
                     /locus_tag="VGA21_04780"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019596750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HEX9649851.1"
                     /translation="MIKILFVCLGNICRSPLAEAIFNSKLERLSLQDKFIVDSAGTAD
                     YHIGENPDARTIKVAKLNNLQIIHKARQITTMDFSEFDYILAMDRSNLENIETLQDGN
                     NNIKILLIREFDKKKELDVPDPYYGGKDGFKIVYSILDSAIENFLQFLRKKHNL"
     gene            complement(12594..13856)
                     /locus_tag="VGA21_04785"
     CDS             complement(12594..13856)
                     /locus_tag="VGA21_04785"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009581138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEX9649852.1"
                     /translation="MKVFKFGGASVKNADAIRNMSQILMTYNGKKLVVVISAMDKTTN
                     ALEGILHARMRNENISEQVKKVSDFHLKAIPELFPDGNPELQSVVQYLMDKLNTTLET
                     HDQLDYNKFYDAVVSTGEYLSSAIVCNYLNSIGFISHWMDASKFIRTDASYREGIVNW
                     RLTGNLIRNSFKEILNTKTVVTQGFIGGTEEGQITTLGREGSDYTAAIIASALEAESV
                     TIWKDVPGILNADPKKVPGAVLFPELPYTEASEMTYYGASVIHPKTIKPLANKKIPLY
                     VKSFDHPDEPGTIIHKCKVTTTIPTIIFKENQCLFSFKVLDFTFVNEENLSKIFHELH
                     ELNIRINMMQNSAISFSVVFDFDPIRVDLLKERLRDNYTFLYNKNLTLITIKNSNEQL
                     EKEYSGKRKIYLMQRSRKNVRYLVAEKS"
     gene            13941..14933
                     /gene="fbp"
                     /locus_tag="VGA21_04790"
     CDS             13941..14933
                     /gene="fbp"
                     /locus_tag="VGA21_04790"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002695657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HEX9649853.1"
                     /translation="MEESKIALPIGTVLDRFIKANEDDFKYASGELSQLLRDIALSGK
                     VVNREINKAGLINIVGGIGSQNVQGEDQQKLDVLANIRFTRALKKGGEVAAIISEEEE
                     EIIETGNLDGKYIVAIDPLDGSSNIDVNVSIGTIFSIFRRKTKQGAPVADIDVLQKGS
                     EQVAAGYILYGSSTMLVYTTGAGVNGFTLDPSLGEYVLSHANMQIPENGKIYSINEGS
                     FNSFETGVRNYVEFCRRNNYSGRYIGSLVADFHRNLMKGGIYIYPRTEKTPEGKLRLM
                     YECNSLAFIAEQAGGAASTGKGRIMDVQPDEIHQRIPFFVGSKNMVEKAESLIS"
     gene            complement(14934..15218)
                     /locus_tag="VGA21_04795"
     CDS             complement(14934..15218)
                     /locus_tag="VGA21_04795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649854.1"
                     /translation="MGDELQIINTQLRTIEKPGYQYEDVLHKLTPVISNLLDTDMNGL
                     MNILYRVDVSEKKFRETFATGKPEKIAEMIARLIVDRHLEKTVTRKKYSS"
     gene            complement(15225..15719)
                     /locus_tag="VGA21_04800"
     CDS             complement(15225..15719)
                     /locus_tag="VGA21_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009581142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5606 domain-containing protein"
                     /protein_id="HEX9649855.1"
                     /translation="MEFKDVASVSGKSGLYKVLKPTRTGVILESMDESKSKLVTSLNA
                     RVSILDEVSIYTNTKEGSVSLKEVLRKIYKEFDSDPGVDAKSSPEELKAFLKFILPDF
                     DENRVYPSDIKKLVQWYKTLYVQAPELLKEEVKKEKLKKSVKKKKESGETVKKKVAKK
                     GGSK"
     gene            complement(15751..16353)
                     /gene="yihA"
                     /locus_tag="VGA21_04805"
     CDS             complement(15751..16353)
                     /gene="yihA"
                     /locus_tag="VGA21_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002698289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HEX9649856.1"
                     /translation="MQIKESVFVKSSPGIADCPTTNLPEFAFIGRSNVGKSSLINMLL
                     GRKKLASVSTRPGKTQLINHFKINNSWYLVDLPGYGWAKVSKEKKANWDKMIRIYLSK
                     RENLACVFSLVDSRIEPNKNDLEFIDWMGKNKIPFVIVFTKSDKQSKNKTQSNIATFL
                     KVLKSSWEEIPRALVTSAVDSTGRKEVLDFIGNVLRDLEN"
     gene            16436..18100
                     /locus_tag="VGA21_04810"
     CDS             16436..18100
                     /locus_tag="VGA21_04810"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX9649857.1"
                     /translation="MLKHSCIIWVGLFYFPQNNFGQNPREAERLLLEGNKLMEAGNFR
                     EAGKYYLQSAKTDTLNIEANSKASVSLLNYSKPQALPYLMRVYQMDSTFRPDIRYLIG
                     LSLQSAYRFNEAIRHFSAYREASNNFDSRNNNPSTNETLQRIMECNNGLEFLQNPQPI
                     EIKNLGAAVNSAFADYGPVLDQKESVLIFTSGRKEGNTNLAIESGMYFEDIYYSSLEN
                     NLWTNAKNVGLPVNSPYHESVICLSPGGDKLYIYKDEGEGDIFVSNLLQKGWSEPIPL
                     AVVNSPYSERSMTISAGGNTIIFSSNRPGGVGGYDLYKITYDGKYWSKPENLGYSINT
                     EKDDDSPAFGYDNASLFFSSKGWRGMGGFDIFISKLDGAEPLNLGFPVNTPEDDIYFA
                     PARSGKKAYFASVRSEGYGELDIYSLQSLNGVIGGNVVSNVVNENFQLLTESNLETIY
                     FEFGKTQLLSISEENLKNNINYLNSHPNIEILLVGHTDNIGSEDENSVLAMERSMKIA
                     ILLKQNGISEKRIRLEAKGECCPAVSNADFDKIYMNRRVEFVIVKE"
     gene            18166..18888
                     /gene="ubiE"
                     /locus_tag="VGA21_04815"
     CDS             18166..18888
                     /gene="ubiE"
                     /locus_tag="VGA21_04815"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009183863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052624 - 2-phytyl-1,4-naphthoquinone
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0042372 - phylloquinone biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HEX9649858.1"
                     /translation="MTVVPYKERSESKKQQVATMFDNISKKYDFLNHLLSLGIDISWR
                     RKAIKKLQKLKPSKILDIATGTGDFALEALKLKPEKIIGVDISDGMLTVGREKMVRKK
                     VSHIIDLQSGDSENLQFPDNFFDAVIVSFGVRNFENLEKGLSEMHRVIRPGGMAVILE
                     FSKPEKFPIRQIYNFYFKVILPKIGRLFSGDTSAYTYLPESVQEFPYGKKFVEILGKV
                     GFKYNQCYPQTLGISSIYTGEK"
     gene            18906..19568
                     /locus_tag="VGA21_04820"
     CDS             18906..19568
                     /locus_tag="VGA21_04820"
                     /inference="COORDINATES: protein motif:HMM:NF024957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HEX9649859.1"
                     /translation="MVTLQGLAQKKARKIFNPNYDDRRKITYGFSIGIHNTYLGLKYS
                     DLFVTAELDTVHSILPPASPGFSLGFIVNLRAADLLDVRLTPRVSFNEYQLRYYFTDN
                     TSNRIIVENTMVEFPILAKFKSERRGNFRMYVVGGVSPAFDASGKNDLEDTSTGLNFR
                     KGNLTFEMGFGVDIYYPLFKFSPELRYSKGIINTLGEQNEFSLGISRLSTNIITLFLL
                     FQ"
     gene            19568..20326
                     /locus_tag="VGA21_04825"
     CDS             19568..20326
                     /locus_tag="VGA21_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002696010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9649860.1"
                     /translation="MKKQIALITGSTSGIGQATARLFAENNIELICCGRREDRLEELK
                     GELSGKTGVITLSFDVRDKEAVKKSLESLQASWRKIDILVNNAGNAHGLSFIQEGDLN
                     DWDAMIDINIKGLLYVSRVIIPGMIERKSGHIVNIGSVAGQEVYPRGNVYCASKFAVD
                     AINRGMRIDLNEHGIKVTAINPGLVETEFARVRFKGDEEKASGVYQGYEPLTPEDIAD
                     CILFAITRPAHVVIADMLVFPKDQASSTIVNRKS"
     gene            20538..20888
                     /locus_tag="VGA21_04830"
     CDS             20538..20888
                     /locus_tag="VGA21_04830"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HEX9649861.1"
                     /translation="MRIQRFEDLIAWQKGQDLAVEIYSVFRNSKDFGFKDQICRAAVS
                     VSNNIAEGFDRQSDAEFSRFLFISISSCSEVKSMLYLAYRLAYLDYEQKEILLQKANE
                     ISRVLRGLIKSLAK"
     gene            complement(20981..21469)
                     /locus_tag="VGA21_04835"
     CDS             complement(20981..21469)
                     /locus_tag="VGA21_04835"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002698285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEX9649862.1"
                     /translation="MDNKLRLSFEYDLFDNEDELFNDWKLLLVKAREVMTNAYSPYSN
                     YKVGAALLLENGEIVTGSNQENAAFPSGLCAERVAFFAAGSAHPGVAIKRAVVVARKP
                     GNNNLEVAAPCGGCRQVMLEYELKQEKPIQLLLVYKNSKFLVASSIESLVPLSFNKHN
                     LE"
     gene            21583..22923
                     /locus_tag="VGA21_04840"
     CDS             21583..22923
                     /locus_tag="VGA21_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018477250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HEX9649863.1"
                     /translation="MTKILVIGAGRSSSTLIDYLLRNSGEHSWEVTIADSHIETAKSK
                     AGGMDARFVSFDFTNTLQKEAEISRADIVLSMLPMKFHPEVARQCVHFNKHLLTASYA
                     SAEIRELDAEAKNKGLLLLNECGLDPGIDHMSAMKVIDRIRNNGGKIVGFESFTGGLL
                     APNDNVDNPWQYKFTWNPRNVVLAGTGGVKFLQEGRYKYIPHHKIFRRTEVVHIPGYG
                     YFEGYANRDSLGYRHVYGLDDIRTMYRGTFRRQGFCKAWNIFVQLGACDDSYVLEEVG
                     KMTHRQFINSFLPYIPDDSIELKLAHYMNLDLESDEMNKLRWAGFFSNELVGLDKGTP
                     AQILEHILKKVWTLDQEEKDMIVMWHKFNFLEGAKMRQIHATMVAVGDDRINTAMSKT
                     VGLPMGIATKLIATGKIKSSGVKIPIEAEIYEPVLTELYKEGFVFIEEEIKPKK"
     gene            complement(22934..23410)
                     /gene="rnhA"
                     /locus_tag="VGA21_04845"
     CDS             complement(22934..23410)
                     /gene="rnhA"
                     /locus_tag="VGA21_04845"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008198528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HEX9649864.1"
                     /translation="MITIYTDGASKGNPGPGGYGVVLKFNGRRKEISEGFKLTTNNRM
                     ELLAVIAGLEALKSNKYPVKIYSDSKYVVEAVEKKWVWTWIRKGFKGKKNEDLWRRFI
                     PLFEKYKPKFIWIRGHSGNTENERCDELAVDAAAGRSLKKDKGYENSMNVDSGLGI"
     gene            complement(23407..24468)
                     /locus_tag="VGA21_04850"
     CDS             complement(23407..24468)
                     /locus_tag="VGA21_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009581177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX9649865.1"
                     /translation="MVSFYPGPSRVHSQIPEYFQDAYNSGVLSMNHRSPEFMELYRNT
                     VALLHEKLNIPKSYKIFFTTSATENWEILAQSLTKKSSAHFYNGAFGDKWYKYANYLH
                     PGCSKFEFGVNDSIDIRKLKIPAKTEMLCFTHNETANGTQIESGILSVARKKFATSLI
                     CVDVTSSMGGIELNFRLGDAWFASVQKCFGLPAGMGILVCSPALIERSGKIGEKGRYN
                     SFNFIFQNSQKWQTPFTPNVSNIYLLNRVLKNSDNIKQIEQKLNSRYESWVEIFTQTK
                     AFQFFVKNDEVRSRTVLAIEGAPKAIGEVKLLAKKEGFLLGSGYGELKESTFRIANFP
                     ALLDIEVNGLQKFFKNNYL"
     gene            complement(24468..24890)
                     /gene="aroQ"
                     /locus_tag="VGA21_04855"
     CDS             complement(24468..24890)
                     /gene="aroQ"
                     /locus_tag="VGA21_04855"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009184349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HEX9649866.1"
                     /translation="MKIIIINGPNLNLVGTREPSVYGTKSFDNYFRELAKEFSDTTLE
                     YYQSNVEGELINKLQEVGFTYDGIVLNAGGYSHTSVAITDAIAAIETPVIEVHISNIH
                     AREEFRHESLLSKNCAGVITGLGLDSYRLAIVYLIMKV"
     gene            24911..>25446
                     /locus_tag="VGA21_04860"
     CDS             24911..>25446
                     /locus_tag="VGA21_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011586063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEX9649867.1"
                     /translation="MSWDNTITHFKNYLKLERSLSDNSIEAYTHDVVKLRQFLEISNV
                     KRGPTEVTNIDLINHLEFLNELGMSAFSQARMLSGLKSFFKYLLFEDLIKQDPTALIE
                     GPRLGRKLPDTLSFHEIENLLNAIDLSTAEGARNRAMIETLYSSGLRVSELINLTLSN
                     LHSDIGFLKVVGKGNKER"
BASE COUNT         7717 a         4858 c         5214 g         7657 t
ORIGIN      
        1 atttgccagg taacgctgcc cgtagaaata tgattgttat tcaagtcaag ggttttaacc
       61 tcgcatatta tatctacgga ttcttcattt tccaatggaa taaacaccct ttcccgcatt
      121 tccttttctg aaagcccaaa ctttgcaatc acactggttt ttccctggta atgatattcc
      181 atctgtagat tccttaaaat tatccggtac ttattggcgc cgaatttact gatcagaaaa
      241 actccggtag tgaattctgt taaggttgct aacgcgcagg catgtacact cctgatatgg
      301 ttaaaattca gggttttgaa aggcaatttg gtttcaatgg actcatcggt taatttcacg
      361 atcttaaaac cgtggggcct gttaaaagga accatcctgt tcagaccaaa gttaagtaac
      421 cataaataaa aggtggaagt ttttgctttt tcaataattt tttgaatatc gaacataaaa
      481 tttaagagta ataggacaga tgcaagctaa cgaattaaag ataaaagtta tggtgttggg
      541 ttgtggattg gtaggaaaaa ccattgccgg tgatctccgg aagaattttg aagttactgt
      601 agttgatttc aatactgaaa ttcttaagga gatgaactca aatggattcc ggaccttaca
      661 ggccgatctt agcgactcaa atactatcaa agaaataatt aaagatcatg aactcgtagt
      721 tggcgccgta ccgggattca tcggctataa tttggtaaag gcagtaattg aagcgggtaa
      781 aaatattgta gatatttctt ttttccccga agatcagttt acattggaac aacttgccat
      841 agacaaaggc gtgacggcga ttttggattg cggtcttgct cccggaatgg gtaacatcat
      901 ccttggctat cacaatgcca atatggaagt tcacgaatat gaatgcctgg tgggaggttt
      961 accggtggaa agagaatggc cttatgaata caaagccgta ttttcacccg gtgatgtaat
     1021 tgaggagtat gtgcgccctg caagatttgt aaagaatgga ggtattgtga ttcgcgaggc
     1081 cctctctgat cctgaattgg taaattttga aaaagtggga acactggaag catggaattc
     1141 agatggctta cgatccttgt tgaagaccat gaaaattcct aacatgatcg aaaaaacctt
     1201 gcgatatccc ggcactattg aatatttacg cgtcctcaga gatactggat tcttttcttc
     1261 agaacagatt gaagtaaagg ggatcatgat tcgacctgtt gatctcactt cgaaattact
     1321 tttccccaag tggaagctaa agcccgggga gaaggaattt accgtaatgc gaatcatcat
     1381 ttcaggaatc gaaaatgggg acagggtaaa atacacctat tctctttatg atgaatatga
     1441 cccggaagca aagcaaactt ccatggcaag gacaacagga tttacctgca atgccgcagt
     1501 taatttattg gctcgcaaaa agtttgatca aaaggggctg attctacccg aatatcttgg
     1561 taccgaccgg gataattttg atttcatttt aaattatctg aaagaaagaa acgttgatta
     1621 tcaaatgaaa cgcggtgtgg aataagaaag gctttgtgat cagatcgatt aatactatct
     1681 tgaaagctat ttttctacta aaaaaagact gatgacgaat tctaaagccc agccgtcctt
     1741 gatgatagcc ctggttcccg taattgcttt gattgctatg ctggcagcca gtgtgatagt
     1801 ctttggtgac tctgccattg agggagcaaa tcaaatgtca ttgttgatgg cagcgctcgt
     1861 ctgcgggatc ctgggaattg gattgggtta taaatgggac gagcttcaga aaggaattgt
     1921 taaaagcatt tcctcggcaa tgtctgctat tctaatcctg cttttcatag ggtcactggc
     1981 gggcacctgg ttattaagtg gaattgtgcc ggcaatgata tactatggac ttcagattct
     2041 taacccggcg atctttcttt ttgccgcctg cctcgtcagt gctattgtat ccctggcaac
     2101 tggtagctca tggtcaacaa ttgcaacttt aggaatcgcg ctactgggaa ttggtaaggc
     2161 tatgggaatg tcagaaggaa ttattgccgg tgccataatt tcaggtgctt atttcggtga
     2221 taaaatgtca cctctttccg acacaactaa cctggctccg gccatggctg gtacagatct
     2281 tttcacccac atccgttaca tggtttatac gaccggacca accatgacaa tcactttaat
     2341 aatttttttg gtgatgggct taacaatcga tacaagtatg accgacgcga gtatgaatga
     2401 aataatggct tccattgatg gtgcattcaa catcaacggc tggctatttc tggttccgct
     2461 gctggtgatt gtcttgattg tgaaaaaagt tccggcgtta ccggctctca ttggtggaac
     2521 attgctggga ggcgtcttcg cgattatttt tcaaccccag gcaatagatt atgtttcagg
     2581 aatccccggg gcagggataa ccagcgcatt tgttgctgta tggaaatcaa tgtatggcgc
     2641 tataagtatc agtactgata atgaaatggt aaacgaatta ttatccaccg gaggtatgtc
     2701 cggaatgttg aatacaattt ggttaatttt atgtgccatg atttttggtg gagttatgga
     2761 agcatgcggc atgttgcaaa ggattgccgc ttttattgtt cagggtgcaa aatccacggg
     2821 ctcactaatc gcggccacta ccggttcttg tattttcttc aatattacag cttctgatca
     2881 atatttggcc attgtcgtac ccggtagaat gttcagggga gcatttaagg aaagaggact
     2941 taagcctgaa gtgctaagcc ggacacttga agactctgga acggtgacct cagttttggt
     3001 gccctggaat acttgtggtg caacacaatc aacaattctc ggagttagta cttttgccta
     3061 tgctccatac tgtttcttca atattattag tccgctcatg accatattat ttggctattt
     3121 gaatatcaaa ataagaagat ttgttactga aggagaagtg aaaaaggaag attagttatg
     3181 tttaagcctg agataaccag ggaagaattg aatgagttac ccctcggaag ttttaacgga
     3241 gatatccatg ttatcacgga ggccggccaa ataaaaagca ttgtccaaaa gctaagaaaa
     3301 ttaaattttg taggttttga tacggaaacc aggccggcct ttgttaaagg acagaagttt
     3361 ccaatttccc tcacccagct tgcttgtgat gatgaggtat ttctaattag aaacaaatat
     3421 accggatggc ctgattcact ggcgcatttt ctagccgatc ctgatgtcct taaaattggt
     3481 gttggaattc gagatgatat caaatctatt caatcggaaa ttcggagcaa ctggagcccg
     3541 gaaggatttg tagaactgaa ccatattgta aaagagcttg gtattgtaag ccagggaatt
     3601 cgaaaactgt ccggactggt tttaggattc agggtttcaa aatctgcgca aacatcaaat
     3661 tgggaaagag aaaatttgga tgaaaagcag ctgcgttatg ctgcaactga tgcctggtgt
     3721 tgccacagaa tttacagtga actactggcc agaggttatc tcaattgagt gaataatttt
     3781 tttttcttgc agtcgtctta atttatctgc cagttcctgc ttatttttta ttttaactcc
     3841 gaacctgatg gtgcatattt ctttatattc ctgctcataa ataataactc cagccttatt
     3901 cagttgatac attacctcgc tggttcctga gtatggaaaa gtgatagata tttcaattag
     3961 aatttccact tcaataataa tggaaccatc aagtgccgcc tctgatgaga acctgtaagc
     4021 atttatcagg cctcttttac caagcttcgt ccctccaaaa taacgcacca caatcaccaa
     4081 tacattggtc agattcttcg acctaatcgc gccaagaata atatctcccg ccgtatgatt
     4141 gggttcccca tcatcatttg agcgaaattc ctgtccctgg ggacccaaaa catacgcata
     4201 gcaatggtgc cgggcatcgt gatattttga tttcagttcc tgcaaatgcg accttacctg
     4261 gtcagccgat tgcaccggaa aagcaaaagc aagaaacttg cttcctttgt ctttgaacaa
     4321 tccctttgat ttctctttta tagtaaagaa attttctttc atctacctaa gaaattgaaa
     4381 tatttcctga tgagcaatta atagcagggc aagaattcca aaacctatcc caattacttt
     4441 gaatttgttc cacttttcct tgaagataaa tcctgaagca acggtcgcaa aaataattat
     4501 cccgatattc actaacggaa aaacaaaagc accatcatta ttgaaatggc ggagagtcga
     4561 aagtaatagt actattgtaa atacattgat gacacccaat gttactcccc agggtattga
     4621 gccggattca aatttcttct ttccaaaaac aaaggcaatt gttccaagta aagcagctga
     4681 aaagaagaca acaattgtaa aatccaaact ctcctcagag ctgattaatt tcagagttgc
     4741 ataattaagt gaggtgtcaa ttactccgct tagaaagaaa accgaaatcg gcaataatac
     4801 aaaatgtttg tttcggggaa tcaccacatt caattccttc ttcaggccgg aggtgataat
     4861 tgcaacaaaa gtcaagaaaa gtcccaaata atttagccag tcaaagtttc tcagaggaga
     4921 ttgaaaaata accaggctaa aaaatacagg tatagccagg gatactttgc tggaaagaga
     4981 ggcaatagag acatcgaaaa cctgggacgt gcgggcaatc aggaaaaatg ttataacaaa
     5041 tatacctcct aataaaagtg caaaccaaat ccagcttttc aaaccttctg gggtagcaat
     5101 ttgaattcca cctgaaataa ttaaaccgat aacaacgcag gtccagtaat taataactat
     5161 tgtttgaaaa gtatttacca gaaattggga taataatcta aataaaatca ggatgaaaac
     5221 atttagaatt acaaccaaca ggagtgaaat catacgtcaa acataaacaa taaagaattg
     5281 agaaataaag cagtttcaaa tttcgaaatt tatagagcaa aattctaaag agatgggata
     5341 tcaatttcaa cagctttctg aaccaataaa tgttccggat ttttccttat tcaattccat
     5401 tgactttctt agccggcacc aaagcaatct gttgcaattt ctgataaaga gaaaatctga
     5461 aatagtgggc gtcattcatt tcaacctgat aggtgacaag gcgctaagtc cgtggaaagc
     5521 cagtttcggt ggatttgagt ttagcaaaga attgccctat tctataataa gtcactttgt
     5581 aacctgggtc atcagccagg tagtaagaaa gaatattgcg gaaatacaaa tcacattccc
     5641 cccggatttc tataaaaatt ctgcctacga aatcatgagc gatatcttgc aagactgcga
     5701 ttttcaggtt tgtgtagaaa ccggccaggc attggtatgc ggcgagaaga attttagcca
     5761 gcttattaac aggtttgaga gatcgaaact tgaacgaagt ttgagcgatt cctttagatc
     5821 atggaaaggg atcggggagt taccggaagc agcatttcaa atgattgtga agaacaggga
     5881 aagaaaaaat attccggtta aactcgaata tgctgagttg aaggaaaact tcgtgaaatt
     5941 ttctgataat ttccattggt ttggtgtatg gaagggagat gaaattgccg ctgcttcagt
     6001 tggaattcgc gttcaacctg aaattatgta tcatttcttt cccggccatg ataataaatt
     6061 cgacgcgtgg agtcctatgg tattattatt gaatgagttg tatgggtatt gtcttaagaa
     6121 agaaataaaa tatttagacc tgggaatttc aagcgttgac gggaaaatta atcagggact
     6181 ctatcaattt aagaaaaatc tcggggcaat ggaatataaa caagcgaaat tcaaatggac
     6241 agcggaatag ttaattttcc gaaagtttca gtaatctgta ccagctacaa tcaccaggat
     6301 tatgtattgg aagcgctaaa ttccgtaatg agccagacct atccaaatat tgaattgatt
     6361 gtggtggatg ataattcttc cgataaatct gccaatcgga ttctgcaatt tcaagctcgt
     6421 aaccctgaga cactggtaat tcttaacgac aaaacgctgg gtatttgcaa atcattcaac
     6481 aaaggtttta gaaattcatc gggtgcatac ctgatagatc tggcagcgga tgatatcctt
     6541 cataatgatc gaattgaaaa gggagtgcaa tctcttgaga atctgggcaa accttttgga
     6601 gtcaattttt gtgattgcga aattattgat caagcttctg aaacacttgg atatcaatac
     6661 aagggaaaaa ttaattataa caaagtacca caaggtgaag tctttgcttc tttactttcc
     6721 aggcactgga ttagcagccc cacattatta ttcaaaagag aagtaattga aaagttaaga
     6781 gggtatgatg aaactttgtc ttttgaagat tttgatttct gggtaaggtc ttcaaggtat
     6841 taccactact cattcactcc tgaagttctg gtaaccaaaa gaaaactgca cgactccctt
     6901 tcatccgtac aataccaatt acataatagt cacatcaggt ctatcattta tgtctgcaaa
     6961 aaagcagttg ttcttgttcg tgagaagaga gaaagaaaag ctttgcaaaa aagaatagta
     7021 attgagatgc gaagggcaat caaaacgcat aatatccggg aagcaaaagc ttttctgcaa
     7081 ctccttcgat caacgggaag ctttttcagg ttctggggat atcatttcct tgtatcctca
     7141 ttccgcagca tgctcaggta acttctatag cgactttcaa aaatagttcc ttcctccacc
     7201 gcggctttta tggcacactt cggttcgtga taatgtgtgc aattatgaaa tttacattta
     7261 cctctcattg ccctcatctc cggaaaataa tctgacaact caaactcttc aatgtcaatt
     7321 aatccaaact ccttgattcc cggtgtatct ataataaaag tttgtggaaa tacttcaaac
     7381 atctcggcga aagtggtagt atgaacacct ttagttgaaa atgatgaaat atcagcagtc
     7441 ttttgtgcga tattttctga ccatacatta aggagggtag atttaccaac tccggaatgg
     7501 ccggatagca ggattacttt cccggtaaga ttttccgcga gtttatccaa accggttccc
     7561 tctttcgctg agatctcaaa acatttgaat ccgattaaag aatacatatc ttttaatctg
     7621 gcttgctctt ccctggattt tgtatcgtgt aaatcaattt tgttgaagat caaaaatacg
     7681 gggatgcgaa aagattccgc agatacagta aaccgatcta taaacccggg ggaagtcctt
     7741 gggctgaaaa gtgtaaccat taatgcagct atatcgatgt ttgccgcaat aacctggctt
     7801 tcctgggttt tcctgcttgc tttacgagtg atgaaatttc gtctgggtaa aatatttgta
     7861 ataacgccgt tagatgcgct gccggtttct ttctcaaatt caacttcgtc tccaacggag
     7921 atggggttag tggctgtgga atcttcaagg cgcagctttc ctctggtgcg gcattgccaa
     7981 atagcaccat cataactaac taagtaccaa cttcctgttg atttcatcac tttgccaatc
     8041 atgcttccaa aagtttcttg ctgaaataaa aaattctttc cgtggcacct cttttggatt
     8101 caaaatactg ccggataatt acttccaaag attcttcttt accttccagg ccattgagtc
     8161 gatccgcgat ttcatttccg cctgaaaccg gaaaggcagc acctaaattc gctaactcaa
     8221 cagcttcatt gaattttttg taattcctgt taccaaaaaa aaccgggatt ttgtaaacag
     8281 aagcctccag gatattatgc aatcctttgc caaacgctcc acctatgtaa gcggcttcgg
     8341 cgtatccata gaccgaagca agcattccca ttgtatttag gagaagtata aagtttccat
     8401 cttcattttc cagatctgag tgggtattac agggatagtt gagtttttct ttaatggcat
     8461 tcagattctg taagctgata tcatgtggag caagaatgaa ggtataactt tcctttaatc
     8521 gtgcaattaa ttcatttata acttcgagat ccgattccca aatgcttccg cagacaaaaa
     8581 gttttttgtt ttttttgaac ctgtcaattt gttggatctc tttcctttgc gaggcgattt
     8641 ctaaaacccg gtcgaatcgg gtatcacccg ctaattcaac attttttatc cctattgaat
     8701 taagtaagtc cgcggattcc tgattttgaa caaaaaaata agtaaaatta tttaaaattt
     8761 ttcgattgaa tcccccgtac cactgaaagt atacctgact ttttctaaaa atagcagaga
     8821 aagaaagcat cggaagcttt cttttcttta tttctgaaga ataataatac cagaattcat
     8881 acttaacaaa aaatacaaga gaaggattta tggtatcgag aaagaagctc gcatttttac
     8941 gactgtcaaa aggcaggtag caaatataga atgagggctg ataatttttc cggatttcat
     9001 aaccggatgg agaaaaaaaa gagattaaaa ttttgtattg gggaaaattc ctgttaaaca
     9061 attctatcag cggccgtgcc tgctcaaatt caccaagcga agcggcatga aaccaaacta
     9121 ccggttcctt gttttcttga aatttctgtt tgatcgctgc tctccagttt tttcttcccg
     9181 tcagcataag tttagctttc tcaacaaaag gacttgttaa aaccaggaac aacttgtagg
     9241 aaaaaagaaa aattgaatag aaaaaatttc ccattaaggc agaaagcaaa ttatatattg
     9301 gaatgctgta tcagcaataa gaatcattga attattgagt aatttgcatg gaaaattagt
     9361 taaaattaat aacaattgaa tgactcaggt aactttcaaa ggaagtccgg ttgaacttcc
     9421 gggcaatatt ccatctgtaa atacaatcgc ttccgacttc gcctatgtca agcgcgacct
     9481 ttcagagtcc aggctctcca aattattagg taatgtaatc gtactgatgg cggtacccag
     9541 ccttgacacc ggaacctgcg caaaggaaac aaagaaattc aatgagaaac tggcaaatct
     9601 gacaggagtt ttagggattg tgatttcaaa agatctgcca tttgcattgc gaaggttttg
     9661 tgaggtggaa ggaattcaga atgtggatgc ggtctccgat ttcagatata atgaaatggg
     9721 caagaattac aatattgaaa tgttggatgg cccgatgaaa gggttaatgg ccagagcggt
     9781 gtgggttatt gacagggatg gtaaaattgt gtattccgaa ctgatgaaag aagttgttct
     9841 ggagccggat tacgacaaag tattagaaac tatccattca ttgttataat ttaacttaat
     9901 atttgtgaaa ggaagaaata aataaacgcg ggcaagagta tttaattttg ctttggctgt
     9961 tgcaattttg atggttaaca aacgtacaga aaccaaagaa atttgtttta tggattagca
    10021 atgggattca ctgtacttag gcagtcattg gtagtggatt tgattagcgg ttccttctta
    10081 aatttatata taatgaaagc taaggttaaa agaaatctgg tggttgttat cttgaccatc
    10141 ctttttatag tccccaacca aaaaggattt gctcaacttg accaattgga taaattacta
    10201 caggctggtg aagaagatgc aaacgcttta ttgagtccgt acattgctcc ctttatgcgt
    10261 ggctttggtt atggactggc aaatagctgg tacaataccg cggaagttca caaagggttt
    10321 ggatttgatt taatgattac cggcacactg gcttatgttc ctggtgaaga cctcaaatat
    10381 aatgtacttg atttaaacct gcaggagata gaaatagtga gtcctcgtga tggagaagtc
    10441 ccaactgttt ttggtgatga cctgataacc ccccaatacc aattcaaatc caccccggga
    10501 ttaacatttg atggtccaac cgggttaaat cttcaagatg aaattggagg caaatacatt
    10561 cctacaccta tggtgcagat tggtatcggc ttcccgctcg ggataacaat tcgcgggcgt
    10621 tatatgccgg aagtggacgc cggccagttc aaatccagtt tctggggcgt tggcatccag
    10681 catgatatta aacagtatct cggtgctgtc gataaaatac cctttgacct ttccgttttg
    10741 gttgggtata cgcaattgaa ttcaacgatc ggactctccg gagcaattga tgacggcggg
    10801 gccggggatc aggaaggggt gataaaagcc aatgcatgga cctaccaggt tttgatttca
    10861 aaaaatttat ctatagttac attctatggt ggtgtgggtg caaataccct gcggtccaga
    10921 gttcaaattc tgggtactta tatccttacc gatccgggaa ttattacgct aacggaccca
    10981 atcaatcaaa aatataagga tggtggagtg agaggaacgc ttggaatgaa attgaagctt
    11041 ggccccatca ccctgcatgg tgattacact tttcaaaaat acaatgcact taccgcgggg
    11101 atcggagtaa gcgtcagata atcaagattg aattaggatg tttgactaaa tatgcatttt
    11161 caagaaattt attctgattc aggaatttcc aaatcaaaat ttgcaaaatc accttccctt
    11221 aaactccggc ttccttttct caataaaggc gctcattcct tctttctgat cttcggaaga
    11281 aaaagctaaa taaaaattct ttctttcaaa gttcaaacct tcatcgagat gtgcctcgaa
    11341 tgatcggttc acagcttcct tagctaacat cgcggctacc ggcgacattt cggcaatctc
    11401 tttggcaaga ctcacagctt cttccaggta catttccacc ggaacaactt tgtttaccaa
    11461 tttgtagtct agcgcttcct ccgcggaaag aaattttcct gtaagtatta attccattga
    11521 ctttgctttg ccaatagctt tggtaagccg ctgtgttccc ccggctcccg gcatcacccc
    11581 aatttttatt tccggttggc caaaccgggc ggtttccgag gcaacgatca tgtcgcaggt
    11641 catggccagc tcacacccgc cacccaatgc aaagcctgaa accgcagcga tcatgggctt
    11701 tcgcgttttt ctaatttgat cccatgtgct gaattgatca attttcagca tatcaactgc
    11761 gctcttatcg gccatttgtt ttatatctgc tccggctgca aaggcttttt cgttgccggt
    11821 tataacaatt acccgcactt catcattggc gtccagggat tttagcgtat cgcgtaactc
    11881 agccataagt tgccagttga gcgcattcat ctctttcggc ctgttaatct gtaccagggc
    11941 aaccattggt gcgtatgtgg actgtacatt gaggaattcc atgtttatct ttgtttagaa
    12001 gggctaaaaa aacgaaaaac ctcctttttt caaaggaggt ttcgtcttgc aaacaagtta
    12061 catgaatcaa tctgagctaa ttctttatac ttaacatcgt aagtttgtaa ccagtttggg
    12121 taaaaaatga taaaaatttt attcgtttgt ctgggaaata tatgccgttc tcctctagcg
    12181 gaggcaattt tcaactcaaa attggagaga ttgagccttc aggacaaatt cattgtagat
    12241 tcagcaggga ctgccgatta ccatattggc gaaaatcctg acgccagaac cattaaggtg
    12301 gcaaaactga ataacctaca gattattcat aaggcaaggc aaataactac catggatttt
    12361 tctgaatttg actacatttt ggctatggac cgctctaatc tggaaaatat tgaaacgttg
    12421 caagatggaa acaataacat caaaattctg ttaattcggg aatttgataa aaaaaaggaa
    12481 ttggatgttc ccgatcccta ttacggaggc aaggacggtt ttaaaattgt ctattcgatt
    12541 cttgattcag cgattgaaaa ttttctgcaa ttcctgcgga aaaaacataa cctttagctt
    12601 ttctcagcaa caaggtaacg cacatttttc cgtgaacgct gcattagata aatcttcctc
    12661 ttaccgctat actccttttc taactgttca ttcgaattct taattgtaat tagagtaaga
    12721 ttcttgttat acaaaaaagt ataattatct ctcagccgtt ctttcaataa atcaacccgg
    12781 ataggatcaa aatcaaaaac aactgaaaaa cttatcgcgg aattctgcat catgttaata
    12841 cggatattta actcatggag ttcatgaaat attttgctca gattctcctc attaacaaaa
    12901 gtgaagtcca aaactttgaa tgaaaataaa cattggtttt ccttgaaaat aatggttgga
    12961 attgtggtag tcactttgca tttgtgaatg atggtaccgg gttcatccgg atggtcgaag
    13021 ctttttacat acaagggaat ctttttgttg gcaagtggtt ttatagtttt gggatgaata
    13081 actgatgccc catagtaggt catttctgat gcctccgtgt agggcaattc cggaaataag
    13141 actgccccgg gaaccttttt gggatcagca ttcaaaattc ccggtacgtc tttccatatt
    13201 gttaccgact ctgcctccaa tgcggaagcg ataattgccg cggtataatc tgaaccctca
    13261 cgcccgagag ttgtaatctg cccttcctct gtacctccaa taaatccctg ggtaaccacg
    13321 gttttagtat ttagtatttc tttgaatgaa tttcgaatga gatttccggt taaccgccag
    13381 ttaacaatac cctccctgta gctggcatcg gttcggataa attttgaggc atccatccaa
    13441 tgggaaataa atccgatgga atttaaataa ttgcagacaa tggcggaaga caaatattcc
    13501 ccggtggaga ctacggcatc gtaaaactta ttataatcaa gctgatcatg cgtttcaaga
    13561 gttgtattca gcttatccat caggtactgg acaacacttt gcaactccgg attgccgtcg
    13621 ggaaataatt ccggaattgc ttttaagtgg aaatcagaaa cttttttcac ctgttcactg
    13681 atattctcat tacgcatccg ggcatgcaaa atcccttcaa gagcatttgt tgttttatcc
    13741 atcgcggaga ttacaaccac cagttttttc ccattatatg tcatcagaat ttgtgacata
    13801 ttccggatag catcggcatt tttaacggag gccccaccga atttaaaaac cttcataccc
    13861 tgcgagaata attattgatt aaatttgagg agcaaagatg attttttttg ggataaatca
    13921 atattattca tagctaattt atggaagaat ccaaaattgc ccttccgatc ggtaccgtgc
    13981 tcgaccggtt tattaaagcc aacgaagatg attttaaata tgcatcagga gaactttcac
    14041 aacttttaag agatatcgcg ctttccggta aagtggtgaa tcgggaaata aataaagccg
    14101 gactaatcaa tattgttgga ggtattgggt ctcaaaatgt tcagggggaa gatcagcaaa
    14161 aactggatgt gcttgcgaat atcagattta ccagggccct gaaaaaaggc ggtgaggttg
    14221 ctgcaataat ttctgaagaa gaggaagaaa ttattgaaac gggaaatttg gatggaaaat
    14281 atatcgtggc aattgatccc ctggatggat cttctaacat tgatgtgaat gtttccattg
    14341 gaactatttt ttctattttc agaagaaaaa cgaaacaggg cgccccggtg gcagacatag
    14401 atgtgttaca gaaaggctcc gagcaggtag cggcggggta cattttatac ggctcctcca
    14461 ccatgttggt atataccacc ggtgccggag tgaacggctt tacccttgat ccatcactgg
    14521 gcgaatatgt tctttctcat gcaaacatgc aaattcctga gaatggaaaa atttattcca
    14581 tcaatgaagg atcgttcaat agttttgaga ccggagttag aaattacgtg gagttttgca
    14641 gaaggaataa ttattcagga agatatattg gttcactggt agcggatttc caccgcaatc
    14701 ttatgaaagg cgggatttat atttatccca ggactgagaa aactcccgaa ggaaagctgc
    14761 ggttaatgta tgagtgcaat tcacttgctt ttattgccga acaggcgggg ggtgcagcat
    14821 ctacaggtaa aggaagaatc atggatgtgc aacctgatga aattcatcag cgaattcctt
    14881 ttttcgtggg atcgaaaaat atggtggaga aagcggagag tctgataagt tgattaggaa
    14941 gaatactttt tcctggtgac agttttttca aggtgccggt caacaattaa tcgggctatc
    15001 atctccgcga ttttttccgg tttgcccgtt gcaaaagttt ccctgaactt tttttcgctc
    15061 acatctaccc tgtagagaat gttcataagc ccattcatgt cggtatctaa aagattacta
    15121 attaccggag ttaatttatg aagaacatct tcgtactgat aacctggttt ttcaatcgtt
    15181 cgaagttgtg tattaattat ttgaagttca tcgcccacct taaattattt tgaaccgcct
    15241 ttcttcgcga cttttttctt cacggtttcc cccgattcct tctttttctt aacagatttt
    15301 ttaagcttct cctttttcac ttcttctttc aacaattcag gtgcctgcac atacaaggtt
    15361 ttgtaccatt gaaccagttt cttaatatct gatggataga ctctattctc atcaaagtcg
    15421 ggcaggataa attttagaaa agctttcagc tcttcaggtg aagattttgc atcaacgcca
    15481 gggtcgctat caaattcctt atatattttc cgcagcactt ctttaagcga cacagaaccc
    15541 tcttttgtat tggtgtaaat ggagacttca tcaagaatag aaacccgtgc attgagactg
    15601 gtaacgagtt tggattttga ttcatccatt gattccaaaa ttacacctgt tctggtgggc
    15661 ttcaaaactt tatataatcc gcttttcccg gatactgacg cgacatcttt aaattccata
    15721 ttgtatgatt gctttgctca aatatagaaa tcaattttcc aaatctctta aaacattacc
    15781 aataaagtcc agcacttcct ttcttccggt actatccaca gcactcgtca ctagcgcccg
    15841 gggaatttct tcccaggaag attttagaac tttcaagaac gtggcaatat ttgattgcgt
    15901 tttgttcttg ctctgtttgt ctgacttggt aaaaacaata acgaaaggaa ttttattctt
    15961 tcccatccag tctataaatt caagatcatt tttgttgggt tcaatccggg aatccaccag
    16021 cgagaaaaca caggcaagat tttcgcgctt gctgagataa atgcgaatca tcttgtccca
    16081 atttgctttt ttctctttac tgacctttgc ccaaccatat ccggggaggt caaccaggta
    16141 ccaggaatta tttattttga agtgattaat tagttgcgtt ttgccaggtc ttgtagatac
    16201 tgatgccaat ttcttacgac ccaacagcat attaatcagg gaagatttcc caacattaga
    16261 ccttccgata aaagcaaact ccggtaaatt ggttgtcgga caatccgcaa tgcccggaga
    16321 actttttaca aagacagatt ccttaatttg catgaataaa taacagaact ctaaaataga
    16381 accaaatctg taattacgcg cagattaccc gatatttaca attgaaatga ccggcatgtt
    16441 aaaacactca tgtattattt gggttggcct gttctatttt ccgcaaaata attttggtca
    16501 aaatccacgg gaggcagaaa ggcttttgct ggaagggaat aaacttatgg aggcggggaa
    16561 tttcagagaa gcaggaaaat actacctgca gtcggcaaaa acagatacat tgaatattga
    16621 agccaactca aaagcaagtg tgagcttatt gaattactca aaacctcagg ctttaccata
    16681 tctgatgcgg gtttatcaaa tggattcaac atttcgaccg gatattcgct atttgattgg
    16741 gctgagtttg caaagtgcct accggttcaa tgaagcaatc cggcattttt cagcatacag
    16801 ggaagcgagt aataattttg attcgcgaaa caataatcca tcaacaaatg aaactttgca
    16861 gagaattatg gagtgcaata atgggttgga gtttctgcaa aatccgcaac ctatagaaat
    16921 caaaaacctg ggagccgccg ttaattctgc ttttgccgac tatggtcctg tgctggacca
    16981 aaaggaatct gttctgattt tcacttcagg aagaaaagag gggaatacaa atttagcaat
    17041 tgaaagtgga atgtatttcg aggatattta ttactcttca cttgaaaata atttatggac
    17101 aaatgcgaaa aatgtaggcc ttccggttaa ctccccttac catgaatcag tgatttgtct
    17161 ttcgccgggc ggggataaac tttatatcta caaggatgaa ggtgaaggag atatttttgt
    17221 ttcaaactta ctgcagaaag gctggtctga accaatacca ttggctgtag taaattcacc
    17281 gtattcggaa aggtcaatga caatttctgc gggcgggaat actattatct ttagcagcaa
    17341 cagacccgga ggagttggcg gctatgattt atataaaatc acgtacgatg ggaaatattg
    17401 gagcaagcct gaaaatcttg gctatagtat aaatacggaa aaagatgatg actccccggc
    17461 atttggatat gacaacgcat cccttttctt cagcagtaag ggatggagag gtatgggagg
    17521 atttgatatc ttcatttcaa aactcgatgg agctgaacct ctgaatctgg gatttcctgt
    17581 aaatactcct gaagatgata tctattttgc tccagcacgg tctggaaaga aagcttattt
    17641 cgcttctgtc cgatctgaag gttatggaga gcttgatatt tattcgttgc aaagtctaaa
    17701 tggtgttatc ggaggtaatg ttgtaagcaa tgtagtgaat gaaaattttc aactccttac
    17761 tgaatcaaat ttggaaacta tttattttga attcggtaag actcagctgc tttcaatctc
    17821 ggaagagaat ttaaaaaaca atataaatta ccttaatagt catccaaata ttgaaattct
    17881 tttggttggg catactgata atattggtag cgaagatgaa aactctgttt tagctatgga
    17941 aagatcaatg aagattgcta ttctccttaa gcagaatgga atttcggaga agagaatccg
    18001 gctcgaagca aagggagaat gctgccctgc agtttcaaat gcggattttg acaaaattta
    18061 tatgaatcgg agggtagagt ttgtgattgt taaagaatag agatttttaa cagataggat
    18121 gagttatttt aattattgaa tgaactttac cgctgaatct gagaaatgac agtcgttccc
    18181 tacaaagagc gcagtgaaag caagaagcaa caggttgcta cgatgtttga taatatcagc
    18241 aagaagtatg actttctcaa tcatttgctt tccctgggca tagatatttc ctggcggaga
    18301 aaagcaataa aaaagcttca gaaattgaaa ccgtcaaaaa tccttgatat agccaccggc
    18361 acgggagact ttgcgctgga agcattgaaa ctaaagccgg aaaaaataat tggcgtagat
    18421 atttctgatg gaatgcttac ggttgggcgg gagaaaatgg taaggaaaaa ggtcagtcac
    18481 atcatagatc ttcagtcagg cgattcagaa aatttacaat ttcctgataa tttttttgat
    18541 gctgtcatcg tttcttttgg tgtaaggaat tttgaaaacc tggaaaaggg attgtcggaa
    18601 atgcacaggg tgataagacc aggtggtatg gctgtaatac ttgagttttc taaaccggaa
    18661 aaattcccga ttcgtcagat ttataatttt tattttaagg ttattttacc taaaatagga
    18721 aggctttttt caggagatac ttcagcttac acttatttac cagaatctgt gcaggaattt
    18781 ccttatggta agaaatttgt tgaaattcta ggaaaagttg gatttaaata caatcaatgt
    18841 tatccacaaa cgcttggcat aagctctatc tacacgggag aaaaataatt gttctctgct
    18901 tactgttggt cacactacaa gggcttgcac agaaaaaggc aagaaaaatt tttaacccaa
    18961 attatgacga ccggcgtaaa attacttatg gattttcaat cggcattcat aacacttacc
    19021 tgggacttaa atactcagac ttgttcgtga cagcagaact tgatactgta cattctattc
    19081 ttccgcccgc ttcccccgga ttttctttgg gatttattgt gaacctgaga gcagcagatt
    19141 tgcttgatgt gcgattaacg ccacgcgtat ctttcaatga ataccagttg agatattatt
    19201 ttacggacaa cacctctaac cggataattg tagaaaatac aatggtggag tttccaatac
    19261 tggcaaaatt taaatctgaa cggagaggaa atttcagaat gtatgttgtt ggtggagttt
    19321 ctccggcgtt tgatgcttcc ggtaagaacg atcttgaaga tacatcaacc ggtttaaact
    19381 ttagaaaagg aaatcttact tttgaaatgg gatttggtgt agatatttac tatccgcttt
    19441 tcaaattttc gcctgagtta aggtattcaa aaggaataat aaatacttta ggtgaacaaa
    19501 atgaatttag cctgggaatc agccggctgt caacaaacat aataacgctg ttccttctgt
    19561 ttcaataatg aaaaaacaaa ttgcattaat caccggatct acatcaggaa taggacaggc
    19621 aaccgcgagg ctttttgctg aaaataacat tgaactgatt tgctgtggaa gaagagaaga
    19681 cagattggag gaattgaaag gggaactcag tggcaagacc ggtgttatta cattatcttt
    19741 tgatgttcgc gacaaagaag ctgtaaaaaa atctttggaa tcattgcagg caagttggcg
    19801 gaaaattgat atcctggtaa ataatgcagg aaatgcccat ggattgagtt ttatacagga
    19861 aggagatttg aatgattggg atgcaatgat agatattaat attaaaggtt tgctatatgt
    19921 atcgcgggta attatacctg gaatgattga aagaaaatcc ggtcatattg tcaatatcgg
    19981 ttcagtggcc gggcaggaag tttatcctcg tggtaatgtt tattgcgcat caaaatttgc
    20041 ggtggatgcc atcaaccggg gaatgagaat tgatttgaat gaacatggaa ttaaagttac
    20101 ggctatcaat cctggtttgg tggaaactga atttgcgcgg gtaagattta aaggtgatga
    20161 agaaaaggct tccggcgttt atcagggata tgagccgctt actcctgaag acatcgctga
    20221 ttgcattctc tttgccataa caagacccgc acatgtagtt atagcagata tgctggtatt
    20281 tccaaaagat caggcttctt caaccattgt taatagaaag tcgtaagtcg ttaaaaagtc
    20341 gtaaataagt cgtaagtcgt aagtcgtaag tcgtaagtca taagtcataa gtcataagtc
    20401 gtaagtcgta agtcgtaagt cgtaagtcat aagtcataag tccgtaagtt gttacatgat
    20461 caccattgaa agggatatta atcccaatta atgtgcggtg ttctgctgaa tttgcaatga
    20521 actgtcgcta gagatttatg cgaattcaaa gatttgaaga tttaatagct tggcagaaag
    20581 gtcaggattt agccgttgag atatattctg tttttcgaaa ctcgaaagat tttggtttca
    20641 aagatcaaat ttgtcgtgcc gcagtttctg tatccaacaa tattgcagaa ggatttgaca
    20701 ggcagagtga tgcggagttt tcgcgttttc tttttatttc aattagctct tgcagcgaag
    20761 taaaatcaat gctttacctg gcctaccggt tggcctattt agattatgaa caaaaggaaa
    20821 ttttgctcca aaaggccaat gaaatatcga gagtattgcg aggcttaata aaatcattag
    20881 caaagtaaaa gagacttccg acttacgact tttttacgac ttacgactta attttacgac
    20941 ttacgactta attttacaga cttacgactt acttttacag tcattccaga ttatgtttat
    21001 tgaaggagag tggtaccagg ctttctatac tactggcaac aaggaatttg gaattcttgt
    21061 aaactaacag gagctgaatg ggcttttctt gttttaattc atattcaagc ataacctggc
    21121 gacagccgcc acaaggtgcc gccacctcca gattattatt gccaggcttt cgcgctacaa
    21181 ccactgcgcg tttaatcgct actcccggat gcgcagatcc agccgcgaag aaagcaaccc
    21241 tttcagcaca aagtcctgaa ggaaaagcgg cgttttcctg gttggaaccg gtcactattt
    21301 ctccattttc cagtagcaaa gcggcgccaa ccttataatt cgaatagggt gaataagcat
    21361 tggtcataac ttcccgggcc ttcaccaaca aaagtttcca atcattgaaa agttcatctt
    21421 cgttatcaaa caaatcatac tcaaaactta acctgagctt attatccatt ctattggctt
    21481 tgcctcaaga gcaagttaaa ttattttgtc cgcaatattt ggaaagcctc tttcaaatcc
    21541 tatatcttgg atgccgcagc aaataaattt ccaatttttg atatgaccaa aattctggta
    21601 attggggcag gcaggtcctc atcaaccctc attgattatt tactaagaaa ttccggtgag
    21661 cattcatggg aggtgacaat tgcagattcg catattgaga ccgccaaaag caaggccgga
    21721 ggcatggatg cccggtttgt atcatttgat ttcaccaata cgctacaaaa agaagcggaa
    21781 atttcacgcg ccgacatcgt actttccatg cttccgatga aattccatcc ggaagtggcg
    21841 cgacaatgtg tgcattttaa caaacatctt ttaaccgctt cttatgcttc cgcggaaata
    21901 agagaattgg atgcggaagc taagaataaa ggacttcttt tactgaatga atgcggcctg
    21961 gatccgggaa ttgaccacat gtcggcaatg aaagttattg accggattag aaataatggc
    22021 ggcaagatag ttggatttga gtcttttacc ggcgggctgc tggctccgaa tgataatgtg
    22081 gataatccct ggcaatataa attcacctgg aatcccagaa atgtggtact ggcaggtacg
    22141 ggtggcgtga agttcttgca agaggggcgg tataaataca taccccacca taagatattc
    22201 aggcggacag aggtggtaca tatacctggt tacggatatt ttgagggata tgctaaccgg
    22261 gattcgctcg gttaccggca tgtgtacggt cttgatgata tccgcaccat gtacagaggc
    22321 acatttcgac gacaaggttt ttgtaaagca tggaacattt tcgtgcagct tggtgcctgt
    22381 gacgactctt atgtattgga agaagtgggt aaaatgaccc atagacagtt tatcaattct
    22441 ttcttaccct atattcctga tgactccatt gaattgaaac ttgcccatta catgaacctg
    22501 gatttggaat cagacgaaat gaacaagttg aggtgggctg gcttcttcag taatgaactt
    22561 gtgggtctgg ataaaggaac gccggcgcaa atcctggagc atatactgaa gaaagtttgg
    22621 acattggatc aggaagaaaa ggatatgatt gtgatgtggc ataaatttaa ttttcttgaa
    22681 ggagccaaaa tgcgccaaat ccatgcaacg atggttgccg ttggtgatga caggataaat
    22741 actgccatgt ctaaaactgt tggcctgcca atgggaatcg cgacaaaact aatagctacc
    22801 ggaaaaatta aaagcagtgg ggtaaaaata cctattgaag cggaaattta tgaaccagtg
    22861 ctaacggagc tttataaaga aggatttgtt tttattgaag aggaaataaa accaaaaaag
    22921 taatagctga aaactatata cccagcccgg aatccacatt cattgaattt tcgtaacctt
    22981 tatctttttt cagacttctc cccgcagcgg catcaacagc caattcatca caccgttcat
    23041 tttcggtatt tcccgaatga cctcttatcc agataaattt cggtttatat ttttcaaaca
    23101 atggaataaa cctcctccat agatcttcat tctttttccc cttgaatcct tttcttatcc
    23161 aggtccaaac ccattttttt tctacagctt caacaacata tttactatca gaataaattt
    23221 ttaccggata tttatttgac ttcaatgctt caagcccggc aattacggca agtaattcca
    23281 tccgattatt agtggtaagc ttaaatccct cacttatttc ttttctgcgt ccattgaatt
    23341 ttaatactac cccatagcct cctggcccgg gatttccttt ggatgctccg tcagtatata
    23401 tggttatcac aaataattgt ttttgaaaaa tttttgcaag ccatttactt caatatcaag
    23461 tagcgctgga aaattggcaa ttctgaaggt actttctttt aactctccat atccacttcc
    23521 caaaagaaac ccttcctttt tagccaatag tttcacctcc ccgattgctt taggcgcacc
    23581 ctcgatagca agaaccgtcc ggcttctaac ctcatcattt tttacaaaga actgaaatgc
    23641 tttcgtttgg gtaaaaattt ctacccaact ttcatacctt gaattaagct tttgttcaat
    23701 ttgcttaata ttgtctgaat tcttcaacac cctgtttaat aagtaaatat ttgaaacatt
    23761 gggagtaaat ggagtttgcc acttctggct attctgaaaa ataaaattga atgaattata
    23821 ccttcctttt tctccaattt ttccgcttct ttcaattaaa gccggggagc aaaccagtat
    23881 tcccatccct gccggcaacc caaaacattt ctgaaccgaa gcaaaccagg catctcccaa
    23941 tctaaaattc agctcaatcc cgcccatcga agatgtaaca tcaacacaaa taagcgaagt
    24001 tgcgaatttc ttccgcgcaa cactgagaat tcctgattca atttgtgttc cattagcggt
    24061 ttcattgtgg gtaaaacaaa gcatttcggt tttggcagga atcttcaatt ttctaatgtc
    24121 tatagaatca tttacgccaa attcaaattt tgagcagcca ggatgaagat aattggcgta
    24181 tttgtaccat ttatcaccaa aagcgccatt ataaaagtgg gctgaagatt ttttcgtcag
    24241 cgattgtgca agtatttccc agttttctgt ggcggaggta gtgaaaaata ttttataaga
    24301 ttttggaata tttaatttct catgcaacag ggcgacagta ttcctgtaca actccatgaa
    24361 ttcagggcta cggtgattca tacttagaac cccggaatta tatgcatcct gaaaatactc
    24421 tggaatctga gaatgtactc gacttggtcc cgggtaaaaa cttaccatca gactttcatt
    24481 attaaatata ctatagccag ccggtaacta tctaatccaa gcccggtaat aaccccggcg
    24541 caatttttag ataacaaact ttcgtggcgg aactcttccc gtgcatgaat gttgctgata
    24601 tgtacttcaa ttacaggagt ttcaatcgct gcaattgcat cagtaatggc aactgaggta
    24661 tgagaataac cgccagcatt cagcactatt ccatcatagg tgaaaccaac ttcctgcaat
    24721 ttgttgatta attctccctc cacgtttgat tggtaatact caagcgttgt gtcgctgaat
    24781 tccttagcta attccctgaa ataattgtca aaggatttag ttccgtacac agaaggctca
    24841 cgcgttccca ccaaattcaa attaggtcca ttgatgatta ttattttcat tcttagtaat
    24901 ttggagaaca atgtcctggg acaacaccat aactcatttt aaaaattatt tgaagctgga
    24961 aagatcgctt tcggataatt ccatcgaggc atatactcat gacgtcgtaa agctaagaca
    25021 gtttctggaa atctccaatg tgaagcgtgg accaactgaa gttacaaata tagatctgat
    25081 taaccacctg gagttcttaa atgaattagg aatgtccgca tttagtcagg caagaatgtt
    25141 gtctggtttg aaatcttttt ttaagtacct tttatttgaa gaccttatta aacaagatcc
    25201 aacggcatta attgagggac ccaggctggg gaggaaatta cccgatacgc ttagctttca
    25261 tgagattgag aatttattga atgccatcga cttatctact gccgaaggag cccgtaaccg
    25321 ggcaatgatt gaaaccttgt attcatcggg acttcgcgtc tcagaattaa taaatctcac
    25381 cctttccaat ttgcattcag acattggttt tctaaaagtt gtgggaaaag gaaataaaga
    25441 aaggtt
//
LOCUS       DASWHX010000071        45092 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_1530719, whole genome shotgun sequence.
ACCESSION   DASWHX010000071 DASWHX010000000
VERSION     DASWHX010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 45092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45092
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1530719"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..620)
                     /locus_tag="VGA21_04865"
     CDS             complement(<1..620)
                     /locus_tag="VGA21_04865"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX9649868.1"
                     /translation="MKNFLTFVICLASVSLYGQELEGDKINTDKGDLIIRPVQHGSFV
                     MKWDNLIIYVDPYGGEEFYNELPAPDVIIITDIHGDHMNKTTLEILDVSKASFIVPKA
                     VEEQLDEKFKTNLKILNNGESVKIVGFDILAIPMYNLPESEDSRHTKGRGNGYVLTIG
                     GKKVYISGDTEDIPEMRALRNIDIAFVCMNQPFTMEVEQAADAVLE"
     gene            842..1273
                     /locus_tag="VGA21_04870"
     CDS             842..1273
                     /locus_tag="VGA21_04870"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX9649869.1"
                     /translation="MNIKIEEADERHISSIADLSTELGYPITRAKVTQNMNIIKEKPD
                     HKVWVAKEGEQVLGWCHAAKTCWVMMESMIEVFGLVVSAKSRGKGIGKMLINRVELFA
                     RENGVAEVWIRSNRKRRESHPFYLNLAYTNLKDQAVYVKKV"
     gene            complement(1270..1959)
                     /locus_tag="VGA21_04875"
     CDS             complement(1270..1959)
                     /locus_tag="VGA21_04875"
                     /inference="COORDINATES: protein motif:HMM:NF015146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEX9649870.1"
                     /translation="MTFGKKVIAFNKSLSKDLNVPGSVKILDPFSKPAIRSLSEQFYN
                     KFYNDSNSRIFILGINPGRFGAGVTGIPFTDPINLEKYCGISNDLDKRHELSSVFVFD
                     FINAFGGSTKFYNTFFITAVCPLGFTQEGRNINYYDNKKLQDSVETFIIQKMKTQLNF
                     GACRGTAIIMGEDKNYKYFSALNKQYNFFQELIPLAHPRYILQYKRKQMKKYLNDYLS
                     ALSKSIALFNP"
     gene            complement(1977..2528)
                     /locus_tag="VGA21_04880"
     CDS             complement(1977..2528)
                     /locus_tag="VGA21_04880"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX9649871.1"
                     /translation="MTNPMLKNLLITFICLLVSSGAPAQPGDPDGMPGKSVVILFRHG
                     EVNPPPYTDNPPNPNLNEIGKERAQLLASLVKNAGVTKIYCTDLNRTKETVELIRQLF
                     GLEFTFYGVKDMGVIAEEMKSKPGVYLVSGHSNTTPDMVRLLGGDPGEPINENEFDRL
                     YILTIIGGNASSVMLRYGRPYSK"
     gene            2652..3416
                     /locus_tag="VGA21_04885"
     CDS             2652..3416
                     /locus_tag="VGA21_04885"
                     /inference="COORDINATES: protein motif:HMM:TIGR01200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GLPGLI family protein"
                     /protein_id="HEX9649872.1"
                     /translation="MKKIYLFALFLGQSTLFAQSTQGTIIYEETRQLQFNFGNDARSE
                     EMKSMIPSQVSTYKQLLFTADASIFLKYENLEKQSANEMNFTSASGGNVAIKMKEANN
                     EFFRNIAKGKTVEKIEFMSKDFIISEPKVEFAWKITGEIKTILDYTVMKATIEDTAGV
                     IEAWFTTQIPVPAGPDKYGNLPGLILELSLEGGKRIFTATNINLNNVEEKLIVAPNKG
                     KNVSREEFEKIRAEKMKEMEEMYGGKGRTRMIIDHE"
     gene            3418..6177
                     /locus_tag="VGA21_04890"
     CDS             3418..6177
                     /locus_tag="VGA21_04890"
                     /inference="COORDINATES: protein motif:HMM:NF026255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX9649873.1"
                     /translation="MKQGLIIILFCGFFQAKGQDSNLELTGVVKDSSKTTLSAATVIL
                     LQARDSLMHSFTVTNKDGVFTFKRIKPDEYILQISYMGYATYHKKVEVNSENGIFDFG
                     NIILSSKSQLLDAVDIYAPAIPIQIKKDTIEYNAGSFKTKPNDLVEDLLRKLPGVEVD
                     ADGTVKAQGEEVKKVLVDGKEFFGNDTRIATKNLPADAVDKVQVYNDLSEMSRFTGID
                     DGEREKTINLSLKEGRNKGYFGNVTAGYGTDARYNFKGNINRFRKGMQLSVIGNLNNI
                     NQQSYSNNEFLSMAGGLGGMMGGMRVIRMGGSGSGSNSSTGIATSQMGGINLNTELSQ
                     DTKLYGSYFFNHINNELLKNISRTNFLDDESFDSFQETQTKTGNYNHRLNLNLNTKLD
                     NTQDISFRTNMSFNRGNSDKWDGSLTYDTMEQLSNSSAAINNASGDAGNVNANLIYRK
                     RFGDLGRNFVTRLQVDMGSDDNRNFLTSETNFYGPVFNSANLNQNQIGKSDKLDYGGQ
                     VSYTEPLGRRNYLEINYEYQNYTTDVGKEFYDVVVNDEVFNPTLSTGYNRDYYYHQGG
                     ITYMRSRENSNIRARLDVQNSKLKGQIDDNAQLSRGFFNLLPSASYRLSLSNSSNLDL
                     RYSTNLREPSLEQLQPLVDNSDPLNIYQGNPDLKPQYSHRAGLHYSMFDSFNFINLFL
                     NVNGTYIQNKITNATYVDSLFRRVTQPLNVDYDYNIMAFLNFGTPLKFIRSKINLSGN
                     SMASKSIVYVNQLENNANRYISTASLAIENIKKEHYDFRVGTRWTFNQTRYSENDELD
                     QNFTNQNYFSELSIYLKKWTFNAELNYMLYRGDAFQDEQAIPLLHAYISRTFLKNDRG
                     ELRAYGFDLLNQNTGINRNSDLNYIQEERINALSRYFLFSFTYSLRGLAGGGRGNSIK
                     LRQGG"
     gene            complement(6183..6464)
                     /locus_tag="VGA21_04895"
     CDS             complement(6183..6464)
                     /locus_tag="VGA21_04895"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEX9649874.1"
                     /translation="MRLFWTKKARTRLAEILHYIEEEFGEAARQQFRSRTHEFTNLLK
                     EFPEMGSLEIRDKKIRGFQITKQTRVFYRLKNDKITILTFFGSRQDPKK"
     gene            complement(6461..6655)
                     /locus_tag="VGA21_04900"
     CDS             complement(6461..6655)
                     /locus_tag="VGA21_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008631839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649875.1"
                     /translation="MDRIEDERLLRAIFSFLSQREKSEESRMWNSLTEEQKKEVLKAY
                     DESEDDSSLINDEDAWKSLK"
     gene            complement(6788..7381)
                     /locus_tag="VGA21_04905"
     CDS             complement(6788..7381)
                     /locus_tag="VGA21_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014772156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649876.1"
                     /translation="MKKLLTFSITCISITTICYAQIPTDKELQIKMAVMAGPEELRAD
                     ATVMGFNEKGELEIIREGSNELICRSDDPGSPNFETVCYHKDLEPFMARGRELRADGK
                     GGQEIFDTREAEAKSGELKMPDKPATLHILYGGTDVTYDAAANTLNNVQYRYVIYVPY
                     ATEESTGLPLKPNGPGHPWLMHRGTHGAHIMITPPKN"
     gene            complement(7622..8632)
                     /locus_tag="VGA21_04910"
     CDS             complement(7622..8632)
                     /locus_tag="VGA21_04910"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX9649877.1"
                     /translation="MKAYLLTRHGKPEVLKIHEVPEPQILPDKVKIRVMFIGINYAEI
                     LSRRGLYRWAPKRPYIPGLEGMGEVIEVGSKVEHVKPGQKVIFGSQFGSYAEVICADM
                     HLVFPVFSQFALEENGAFLVNFLTAWVALVKLGKIKSADKVLIQAAGGGVGTAAVQIA
                     KGFGCDIFGTIGSDHKIELIRNLGVAHPINYNKVDFYQFIKEKVEGVDLVLEVVGGDV
                     FKKSLDLLNPFGRLVVIGYAGIPFKKFNPYTWWLTWKHAPKVNILKMVQRSYSISGSH
                     LGYLSGNSKVAVEVEMELRRFVQEKNLKPVIGKIFDFDHLPDAHAFMESRQSTGKLLV
                     KV"
     gene            complement(8842..11076)
                     /locus_tag="VGA21_04915"
     CDS             complement(8842..11076)
                     /locus_tag="VGA21_04915"
                     /inference="COORDINATES: protein motif:HMM:NF039587.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HEX9649878.1"
                     /translation="MINSMVSDKIMNIVTIFYDAGMKSRILIAAFSLLTVSLQAQNVV
                     KINRVQLLVEIDGNPFETAWEQIDPLPVTMYTPVFAGEMTEKTEIRVAYDNNYIYASG
                     RFYDSQPSKILGNSMIRDVDKGGDFFNFLIDSYDDNQTVLGFSLTPSGSKIDTEINND
                     AEGDFALFYNVNWNSFWEGKVTRNEKGWFAEMRIPFTSLKFEDKDGQVQFGIIVHRLI
                     GRKNERHVFPAIRPDWVSGAWKASQAQTILMEGIRKKSPLYVTPYLLGGYQMDYDAYP
                     DVTTQNKKFRKEAGIDVKWGISNNVNLDASINTDFAQIEADDQQVNLSRFSLFFPEKR
                     QFFQERSGTFNFATVGNDRLFYSRVIGIHPSGKPVRMYGGIRLTARAKGWDVGLMDAQ
                     IMADDSIPSQNFGVLRIRKSAINKNSFVGGIFSSRIDDDGSTQLTYGLDATVNLVESN
                     FLTLKVGQSADLASSYEFGNRAFGYLLSENRNSNGLGYQLELAYYGKDFNPSSGFIAR
                     RGVVFSKLMLQYGFFFTSSKIRSITPFIVHNNYHSSSNYNLETTSSSAGVRFTAKTGA
                     RSEIFINRNYDSFSEPFNLSEEVVITAGDYTFYSVTGNYTMFDGNRLRFALNLEGGKF
                     YGGNRYSIMLSPIWTVNKYFDLGFDAIYNRVEFDGRDFEGNIARIRITLALNTHLSFN
                     GFLQWNQTQDLSGINLKLRYNWNDGKDLFVVYNENTHNNDPEVPRVENRALLLKYIYT
                     FSKK"
     gene            11294..11494
                     /locus_tag="VGA21_04920"
     CDS             11294..11494
                     /locus_tag="VGA21_04920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649879.1"
                     /translation="MKPMTEKFLFYLGYVVTILAVLGATFFIWSLGVYTMLKILLLLP
                     LIYISVLLTLSLEDGFKKSRWS"
     gene            complement(11491..12408)
                     /locus_tag="VGA21_04925"
     CDS             complement(11491..12408)
                     /locus_tag="VGA21_04925"
                     /inference="COORDINATES: protein motif:HMM:NF021537.4"
                     /note="predicted Zn-dependent protease with a strongly
                     conserved HExxH motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2268 domain-containing putative Zn-dependent
                     protease"
                     /protein_id="HEX9649880.1"
                     /translation="MKHLFLFLFVAGCSSAQMNRDPAAMEIITSDIDNFWNAFEAAKP
                     EFNPEVFQKLYLDPGSKGVEGFMDNRIRNAEHLAKVVKSHEKYFASLKPSTDSILQFK
                     NEIAESVKNMKELYPDAVFPPVYFVIGAMNSGGTTSRQGLIIGAEMHGLRPDTPKEEL
                     NDWLVTVIKPMSHVPHIVAHELIHYQQKYRSRTLLEQSIKEGSADLLAELISGKHINK
                     FAHTFANPREKELFKEFKEKMDGKDFTGWLYSSMEGRPNDLGYWMGYKISKSYYDQIP
                     DKKQAIHDILTIRDFHEFLERSRYAQKFN"
     gene            12546..14942
                     /locus_tag="VGA21_04930"
     CDS             12546..14942
                     /locus_tag="VGA21_04930"
                     /inference="COORDINATES: protein motif:HMM:NF025092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX9649881.1"
                     /translation="MNLPKYFLNLLLGLCALGQSMAQTFIVAGIIKNEENGESLPYAT
                     VKIKETGKGAVTNVEGYFTLLNLPGDSITIEASYIGFYSREFRILPLNQNFLAITLAP
                     STAQLEELVVTAESYQIMNASEGISFVKLSPKDLSLLPGIGEEDIFRSLQLMPGVGAT
                     NENSSGLYVRGGTPDQTLVTYDGMTVYHVDHFFGFFSAFNPDAIKDVQFYRGAFPAKY
                     GGRTSGVVDLTGRTGSVDKIELDAGINLLSSRLTVSLPLFGKASILLSGRRSYSDFLE
                     SGLYKNIYGLIDSGGQDQDAALDNLDPDFTPTSHFYDLNGKVTYRPGINDNFTLTLYN
                     GEDHLDKSRDINNVITIPNLSIRRLLEIDTEDLTDWGNNGVSLRWARQWHPKFFSNAL
                     VTYSTYFSDFNFDFTFRLSDFDTDTTLILAGFKAFEKNKVKDFTYGLENEFHLSENHK
                     LDFGFQAKNYNLTYQFVRDDTITIYDSRQKSDEIGMFLQETWKIGKSIQINGGIRGIY
                     YDLLEEYFLEPRISFEFSFLKNWKIKGGWGIFHQFVNRVINENVTTGSRDFFLLSDNQ
                     ALDVHRSEQFIIGGSFEKENWLLDIELYQKEMTGLSEFSLRFQRQNEIDPTQLFFKGK
                     GKARGGEFLLQKKSGNYRGWASYSISRIEHTFPDLNAGNSFPALHDQSHEFKMAHIYS
                     TGDLNLGLTFIYGSGKPYTQPESLYEISLLDGRDLNYIHVGTKNANRLPAYHRLDVSA
                     HYTLKINKSNLVLGVSIFNLYNHKNVWYKQFDATDLPILITDVNYLGFTPNFSISFKL
                     "
     gene            14939..15757
                     /locus_tag="VGA21_04935"
     CDS             14939..15757
                     /locus_tag="VGA21_04935"
                     /inference="COORDINATES: protein motif:HMM:NF025420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4249 family protein"
                     /protein_id="HEX9649882.1"
                     /translation="MMKKDCLTIVSLLVVLQACNFDDDPLPVEQLVVEAFLYAHEPVD
                     DIHIFTSIPITTDTAIFPPISNAQVRLIRDEVIYDLIPSAEAGYYHYPDTDLDVKEGD
                     EFLLEIYYDNRAITATTVVPPAPLNVKINHDTIEIPPLVPGVIAELAEIQLNITWENP
                     GEDLHFAVIENIEPLDELELIIPQQFQPVREAFLIIAEPTLDTLQNFNVSSLIYLGTH
                     VAKVYRVNQEYADLYSSRAQDSRDLNEPPTNVVNGLGIFSAFSSEQDVFEVRRR"
     gene            15799..17193
                     /locus_tag="VGA21_04940"
     CDS             15799..17193
                     /locus_tag="VGA21_04940"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX9649883.1"
                     /translation="MLLLITFVNSTMHFQLYRLSRYFFQLALRFFFRRIEVKGLERVP
                     MEGPVIIVANHAASFLDPIVIAAKLKVPVYFIAKARALKRPVPTWFLKLFNVIPVYRS
                     QDDPTLMDKNQTSFEACYKHLEMRGNILIFPEGISHSIRHLLKIKTGAARMALGAEAR
                     NKFNLGLKIIAFGMNYSNQEKFQSRLFVSVSEPINVIEFKDVYLQEEVEGVNRLTEKI
                     ENSINQSIINAGDERIDTLINRIERLMKARILKDWGVKKEILEQDHNVTKRIMQAMDY
                     FLERDQARFSKMENEINQYFELLKDVDLPETIFKSKKLTPQAVLFYISYFIPGLLFFI
                     PGLVGNFIPYKLSILSARGWSRNKEYRGAAGFLAGILIFIFFYCLEFMMLKDFTKSLW
                     IPIVSIIAMPFLGIFTFYYWRKLSRFYCKIIFTFSKRQIFIRRLLARRNSIIRELEFA
                     RQEFLEAERNRLAL"
     gene            17331..17699
                     /locus_tag="VGA21_04945"
     CDS             17331..17699
                     /locus_tag="VGA21_04945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649884.1"
                     /translation="MKKLFLLLFSMAVVQITYAQEKPEEEKDQSFILIYSPGELWDSS
                     KSPSEQQYFAEHSALLRDLRNKERITIGGRYSDKGLIILKSKDETEAKEIAESDVSVQ
                     NKLFKVEIFLLNAFYKGCID"
     gene            complement(17790..18347)
                     /locus_tag="VGA21_04950"
     CDS             complement(17790..18347)
                     /locus_tag="VGA21_04950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649885.1"
                     /translation="MVSIIDLWLPILLSAAFVFILSSIIHMVLPYHRSDFGKVPKEDE
                     MMDALRPLNIPPGDYVVPRAFNSAEMKSEEFKAKVEKGPVIFMTVRPNGMQGMASNLI
                     QWFLYSVIVGIFAAYVAGRALEPGNDYLAVFRFAGTTAFAGYSLGLLQTSIWYGRSWS
                     STLKSVFDGLLYALVTAGTFGWLWP"
     gene            18613..19197
                     /locus_tag="VGA21_04955"
     CDS             18613..19197
                     /locus_tag="VGA21_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009582678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649886.1"
                     /translation="MKRLLTILFVFAVTFSFSQKMKVIQGDLKVLKGQTSINVEYDYE
                     DQAVGKYDRESDYIAERKAKYNADEAGRGDHWAEEWVNDRGNLYQPKFEELLTDRTGW
                     KISQDAQYTIILKTLKTEPGFNVGIMRQPAFIDSEAIIVETANKENIVCRISMLKAPG
                     QDAMGYDYSTGPRIAEAYAKTGKSLGAMIQKSTK"
     gene            complement(19292..20590)
                     /locus_tag="VGA21_04960"
     CDS             complement(19292..20590)
                     /locus_tag="VGA21_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009580230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX9649887.1"
                     /translation="MSFIPEIEKKSTLEIKKFQEERLRELLQYLITHSPYYKKLFIEH
                     QIDASQIKSLEDLQKLPVTTKDDIQNNNWEFLCIEKNAVAEFTATSGTLGNPVTIALS
                     RKDLERLAYNEYISFCCANGTAKDCYQLMLTLDRQFMAGIAYYEGIRKLGATVVRVGP
                     GLPQLQLESIQSLNPTVLVAVPSFLVKLIEFAGKENIDLNQTSVRSAVCIGESIRSPE
                     LGLSILAKKILDGWNIKLYNTYASTEMQTAFTECSAGTGGHLHPELIIVELLDDNNMP
                     VEKGSPGEVTITTLGVEAMPLLRYKTGDISVAYDESCSCGRNTLRLGPVIGRKQEMIK
                     LKGTTIYPPGIFNIMSEVDFVKDYAVEVITNELGTDELRIHIEADSSNKNEFEQKLKS
                     IFKSRLRVVPEIILTSQNKLFEIHFRGNSRKVKRFLDNRK"
     gene            complement(20587..20952)
                     /gene="acpS"
                     /locus_tag="VGA21_04965"
     CDS             complement(20587..20952)
                     /gene="acpS"
                     /locus_tag="VGA21_04965"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:NF013786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEX9649888.1"
                     /translation="MIAGIGIDSVMVSRIAEKIRKEEFKLKVFSANEIEYCEKNTHPA
                     EHFAARFAVKEAFLKALGSGLGISYELNQVQVNHRENGRPEILLSGEFKKLEGKWKKV
                     HVSLTHTDTVASAIVILEK"
     gene            complement(20949..22613)
                     /locus_tag="VGA21_04970"
     CDS             complement(20949..22613)
                     /locus_tag="VGA21_04970"
                     /inference="COORDINATES: protein motif:HMM:NF040521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family peptidase"
                     /protein_id="HEX9649889.1"
                     /translation="MPKNKYLRVLLGIAGGIFSLLLILFIYLYVVATVQPPVPDDLSS
                     LELNPVKTPDGLMTIHNSWFRQSQSGLYELYVEGNPFERGVIIGKLTRELVHYQEEVF
                     TQQIHRLVPNTLYLEFLKYFVGWFNRNLEDQILPEYKMEIFGVSESASHEFDDIAPPY
                     HRLLNYHAAHDIGHALQNMSFVGCTSFGVWGTKSADSTLLIGRNFDFYVGQDFARDKI
                     IAFYNPDQGHKFMMVTWGGMTGVLSGMNDQGLTVTINAAKSAIPSGSATPVSIVAREI
                     LQYAKNISEARHIAEQRKVFVSESFLVGSAFDGKAAVIEKTESETGFYESGKDYIICA
                     NHFQSELLSNTELNLKHMKTSPSPYRHNRMEELLNQKEKISVTDAAEILRNQLGLGGK
                     DIGLGNEKAINQLISHHAIIFQPERLKVWISTSPYQLGKFICYDLNKVFSSSPKTDNV
                     EISDSTFTISEDPFLYSLEFKNYQKFVQFRFPFSDRSNLNPDSLVQWNTNSYLSYMLG
                     GDHFFKEKEYAKAQKFYELGLGKEVSSTQEANYMMNQIKICKEKLQ"
     gene            complement(22606..24117)
                     /locus_tag="VGA21_04975"
     CDS             complement(22606..24117)
                     /locus_tag="VGA21_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009580225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX9649890.1"
                     /translation="MDTQYDVVIIGSGMGGLVCGTILAKEGFKVCILEKNQQIGGTLQ
                     TFVRKKVIFDSGVHYVGGLEPGQNLYQIFKYLGIMDKVKIQKMDEDGYDVIIFENDEK
                     EFRHPQGYENFINRLSEDFPEERAGIIAYCNAVKETCKKFPLYNLRTGGIFEKITSLE
                     IDTKTIIESFTNNAKLQNVLASTNLLYAGEAYKTPFYIHALIINSYIESSYRFIDGGS
                     QIGRFLAKEIIKMGGQIFRKTRVTRLIEEKGEIKYALTEKGDKVFGKTFISNLHPGKT
                     LELTESRLLKPAYRHRIEKLENTMSTFLVNVVMKKNAFPYRNYNVYYFENDDVWEIVN
                     YKEENWPLGYAMFFSNSSKSNGYADGISLMAYMHLEDVQEWADSYNIVSKESHRGEGY
                     DEFKKRKAEILFDVVERRFPGFKDAIDSYYCATPLTLRDYVGTYDGTLYGVKKDFREP
                     MKTFISPRTKIPNLFLTGQNLNLHGVLGVTMSAIVTCGELIDTPALLDKIRNA"
     gene            complement(24117..27956)
                     /locus_tag="VGA21_04980"
     CDS             complement(24117..27956)
                     /locus_tag="VGA21_04980"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEX9649891.1"
                     /translation="MEYFFVGLYDFFQKRRKTFFVVFSIVLLVILGLGSQITIEEDIS
                     RVFPNDRKVEKLNQVLEGSNFLEKMVFTVSLKDSLAAPSPDSLILFTEELVAGIQQSL
                     SEYYSALKYKVDDQVVMEIFDYLIDNLPVFLDENDYQILDSLTTPESIANSMQSNYKQ
                     LITPSGVVSRQIILKDPLGISRPILSKLSQLQYDETYTLYDNYIITSDYKHLVFFITT
                     RYPPNDTGHNLELINGLDQQIGRITEKFPAINATYFGSPAVSVGNALQLRKDTALTVS
                     IMVVMLLIFLVGFFRRKRVTVAILFPVVFGALFSLALISLIKGSVSVIAIAAGSIVMG
                     IAVNYSLHYFVHLRHTANIRDTIRDLAKPMTVGSATTVLAFLGLQFVNASLLKDVGLF
                     AGFSLIGAMLFSLIFLPHFFPATLFRKEPGIVEKGIEKLGFRDTKHSNYIVALIFILT
                     PLFFYYAGQVKFNSDMNKLNFMSDDLKQAQKELNEINQFAFRSVFLVTEGNTREDALQ
                     KNEQLFPVLKNLQNSGLVKKISSVSNFILSDSLQKSRINRWNNYWDENKRASVVNLIK
                     QESSKLGFRAEAFQPAFDLLQRRYTTLDNRKTDEMQTTFFADNFAEQPGRVSIITFVQ
                     VDQQEKNKLYDALAGMNKVVAVDNEILTTMLVEYVNEDFNFILTFTSLLVFFALLVSY
                     GRIELTLITFIPMLITWIWILGIMALLNIEFNIVNVMISTFIFGLGDDYSIFIMDGLQ
                     QKYRSNKNNLISIRTSVILSALTTISGLGVLIFAQHPALKSIALISIIGIVCVVIMSQ
                     TIEPFLFRFFIANRAGKKFPPMTFFGLLKTLFAFSYYVFGALLLTIIGFITNLIPIGR
                     KKLKYLYHVLIRLATVSLVYIMVNVKKRVLRNNHDFKEASIIIANHSSFCDIISTAML
                     HPKLILLTNKWVWNSPVFGRVVRFADYYPVMWGTEEGIEKLKARLAEGYSVIIFPEGT
                     RSYDGKIHRFHKGAFYLSEHLKIPITPLVIHGMNTTIQKGDFYLQDSSYTLKFLPKIY
                     PDDSDFGTGYKERARKISKYFKSEFQAMRAEYETPGYFRYKLISNYLFKGPVLEWYLK
                     IKLRLEKNYQPFESIVPKKSSITDLGCGYGFLCYMLHFLSEERTITGIDYDEDKIETA
                     KNCYSRTEKLNFVYADIVEHELEKSDVIILSDVLHYLNPDQQEKVIQKSVQALNPEGM
                     IILREGITDAGERYRGTKLSEFFSVKIMKFNKSTQSLNFMTGEKIRAEAAKHNLEIEV
                     IDDAKFTSNVIFVLRMK"
     gene            complement(27957..29159)
                     /locus_tag="VGA21_04985"
     CDS             complement(27957..29159)
                     /locus_tag="VGA21_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009580265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HEX9649892.1"
                     /translation="MISQEAVQEQFRKQNTCVIVPTYNNTATLEKVIREILNYSGNLI
                     VVNDGSTQDTSGILKKFPFIEVVSYTPNRGKGIALRTGFKKAMELGYDYAITLDSDGQ
                     HFPEDLPLLLEASKKNPGDLIIGSRNMEQQGIPSKSSFGNKFSNFWFWVETGIKLPDT
                     QSGYRLYPVNRLRNINFITSKFEFEIEVLVRASWNGIKILPVPVKIFYEDKEKRISHF
                     RPIIDFARISVLNTVLVILALLYIKPRNFLRKLRTDTWFTLRNLLASPHETERTKAIS
                     IGFGIFMGIAPIWGFQMLVALALAIVMRLNKALVIVAANISIPPMIPIILYLSYITGK
                     IWMGEQADQLAFSKELTLDSIQSNFIQYVLGSFTLATICGLFFGFVTYFTLKVFGKKK
                     RVVSNANS"
     gene            complement(29159..29521)
                     /locus_tag="VGA21_04990"
     CDS             complement(29159..29521)
                     /locus_tag="VGA21_04990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649893.1"
                     /translation="MLINDFFSLKEVKHSNHTIEAVVSFDETHSIFGGHFEDFPVVPG
                     VCMMQLIKEIMEQELHRSFRLSSATSMKFMTVIDPRQNSEVQANIEYGLNGEGTVDFE
                     AKLYSGETTFFKLKAHLV"
     gene            complement(29508..30137)
                     /locus_tag="VGA21_04995"
     CDS             complement(29508..30137)
                     /locus_tag="VGA21_04995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649894.1"
                     /translation="MRFLLLINCFLLFSCGSAYKTLVPAEGDPGCLQKFKPRFTTNWY
                     EANVDVMGNHISGLMLFKLMPDSSMRIAFTSKSGLKFLDFEFTKNGQFKSHYVIDNLN
                     KKVVINTLRKDFEIILMHSLDEWPILSYSKSDLYYYAVPDPDNEADYYVTSWDCTNLV
                     RVEQGGRKKVLEAFFRNYDNRIPGEITLNHTTFNMKISLIKLDIENANQ"
     gene            complement(30115..30744)
                     /locus_tag="VGA21_05000"
     CDS             complement(30115..30744)
                     /locus_tag="VGA21_05000"
                     /inference="COORDINATES: protein motif:HMM:NF015510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HEX9649895.1"
                     /translation="MRSLILFLWLAPLNLLAQYNNFTEISDISGFKKKFNDESAKIKS
                     ISSNFEQEKELTILSEKIISKGEFRFKRENKIRIEYLDPFYYLMIINGDKMIIKDDQN
                     ETTMNVRSNKLFQQVNNIMVESVNGTIIDNEDFSVRIFEDASRYLMEMTPVDKTLKGF
                     FNTILIYADKESFSVTQILMNESSGDTTLIKFVNRNLNAQLSDEIFTLN"
     gene            complement(30737..31510)
                     /locus_tag="VGA21_05005"
     CDS             complement(30737..31510)
                     /locus_tag="VGA21_05005"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX9649896.1"
                     /translation="MIYRRINITFLFIFSISFLIDWFYFPLPWWIYFGATLVYLGIVT
                     YGVVKIDSQFFIPVIFKAGILKSEVAITFDDGPHPQRTPYILDILKEYDAKAAFFCIG
                     KNVDENPDIVRRIHKEGHLLGNHSFFHSDFFDFQSTKKMEEELSMTDDAIKEVSGRKP
                     RFFRPPYGVTNPRLAKVVKNRNYVSVGWSLRSLDTIIKNEKRLFNKIKKRIKPGDIIL
                     FHDHSVVLTDILPEILEYIRNIGLKIVRVDELIEEKPYA"
     gene            complement(31507..32562)
                     /locus_tag="VGA21_05010"
     CDS             complement(31507..32562)
                     /locus_tag="VGA21_05010"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HEX9649897.1"
                     /translation="MSVFIKGMAAISPQKTFENNSFLSEIQEFIGNQLNAVEPSYEGI
                     IEPRAIRRMSKIIRISVAASNKALEEAGVEIPDGIITGTSYGCMADTENFLRKMIDSK
                     EVALNPTPFIQSTHNTMSGQIALLQHCNGYNSTYTQGGFSFENCLVDASLLLADNPGW
                     NLLVGGVDEIIEVSHKILNRFDLYRKENVSNLSLLNLPAEGTMDGEGCTYFVLSGNKQ
                     NALAEIKSIKTIYKPANASHVIESLLKFLNENNLNPTEIDVLLTGRSGDTRYDGIYEK
                     VENIFPESSLLFFKHLSGEYPTASAFATWVGTNALEHNQTPEILARKLSGKTSIRNVL
                     IYNNYFGVYHSLILLQK"
     gene            complement(32564..33763)
                     /locus_tag="VGA21_05015"
     CDS             complement(32564..33763)
                     /locus_tag="VGA21_05015"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009580254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HEX9649898.1"
                     /translation="MKSKAYAAGLGVISAIGKDLAENLEALKNHQAGIGPITLFESIH
                     KNKLPVGEVKVTNEKLASSLKLPTTLTRTALLGIHAVKEAIQSSGIPELRKWRCGIIS
                     GTTVGGMDKSEQYYETFMRNEDSAPLHQIKNHECGKITELIASHFQIQDFISTINTAC
                     SSSANSIMLGSKLIANDQLDIVIAGGTDALTRFTLNGFNSLMILDSEPCRPFDETRKG
                     LNLGEGAGYIVLVSEKVLEQEKVRPLAIVSGYANTNDAFHQTASSPEGRGSFSAMKSA
                     LEMANLMPTDIDYINLHGTGTQNNDSSEGIAVERLFGENVPKASSTKSFTGHTLGASG
                     GIEAVYSIASIQSKVVYPNLRFNHQMSEIKFAPLKSFQKGVEVNHVMSNSFGFGGNCS
                     SLTFSSC"
     gene            complement(33760..34026)
                     /locus_tag="VGA21_05020"
     CDS             complement(33760..34026)
                     /locus_tag="VGA21_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009580253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HEX9649899.1"
                     /translation="MEQLMQTLKVQIIEALNLRHLKPEDIKDDEPLFVEGVGLDSIDA
                     LELIVLLQQHYGVKVEDPSQGPQIFRSVKSMAEYIENNKKPQSA"
     gene            complement(34026..34487)
                     /locus_tag="VGA21_05025"
     CDS             complement(34026..34487)
                     /locus_tag="VGA21_05025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649900.1"
                     /translation="MDNLSKAGVIASELLLQNENPNQKYKPEDIAVVLSNHSSSLDTD
                     LRYQKSTEKIPSPALFVYTLPNIVIGEICIRNKFKGENAFFIHEAFNPEYMVKYVDLL
                     LQNNQTEACLSGWVEVLGENHDVFLYLVEKKKSDYSFNHSMEDLLKKYNNY"
     gene            complement(34644..35897)
                     /locus_tag="VGA21_05030"
     CDS             complement(34644..35897)
                     /locus_tag="VGA21_05030"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018344671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX9649901.1"
                     /translation="MNSLKTDVLVIGAGPAGSVAAAMLHKKGLKVKVVEKQQFPRFVI
                     GESLLPRCMDAFEEADLLDAIKACGFQEKFGAKFMKDDAICDFNFSQGFTPGYSWTWQ
                     VTRADFDKALTDSLQQRGIEIDFQTGVEKIEFAEDGYSKTTVVDKDGNSSVITARYII
                     DASGYGRVIPRMFDLDKPSGLDPRKAVFVHIQDNRRLHFEEPSRILIIVYAPGTWVWV
                     IPFSNGTTSLGFVGSMDYFEKLEGDINQQFKTLVEGNKYLRERFQDQELVFEPRKLEA
                     WSIKTKKFHGNGFVLAGNATEFLDPVFSSGVMFAAVSGHLAANLVVKKLQGAEVDWET
                     EYTDVMQQGVDTFRSYVNGWYDGTLEKIFFHNQPNEEIKKKICSVLAGYVWDMNNPFV
                     QNHSISLRNVSKFIDFQIKMAELSR"
     gene            complement(35900..36964)
                     /locus_tag="VGA21_05035"
     CDS             complement(35900..36964)
                     /locus_tag="VGA21_05035"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEX9649902.1"
                     /translation="MEFFSKDGKNGITAMRDAQWIALAPFVFQAARVIRDSGILDILE
                     DNSDTGLSVEEVSQKSKYNKYQVQMLMESCLASGILIKKDEKYFVTKVGWFILKDEMT
                     RINMDFTQHVCYNGIFDLEKSLENAKPEGLRFLGPWKTIYEGLSILPGKALKSWFDFD
                     HFYSDTAFPEILPLVFANKPIRLLDIGGNTGKWAIQCIEFDKDVEITLMDLQIQLNEA
                     RKNLESHREKDRVKFYQTNLLENKPFPGGFDAIWMSQFLDCFSEEQVISILTRCKDAL
                     NPDGRVFIVEPMWDQQKFEGGAFSLMQTSLYFTTIANGNSKFFYSKDLFNYLNETGFQ
                     IIKETRDLGVCQSLIECKLA"
     gene            complement(36968..38071)
                     /locus_tag="VGA21_05040"
     CDS             complement(36968..38071)
                     /locus_tag="VGA21_05040"
                     /inference="COORDINATES: protein motif:HMM:NF014819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HEX9649903.1"
                     /translation="MERIWVDAHNIISPLGKTTYENYSSVIDGKSGVAPVQIGINTVY
                     GGKILQRENFLVDNFGVFESLMIQSIQAALSQTSAAPSDKDTIFIISSTKGEIDSLSK
                     PNDLKKVNLFESSRKVSSYFNNPNQPVVISNACISGSLAILTAKRLIEAGLYRNAVVC
                     GCDLISDFITSGFNSLQAISSEPCKPFDANRKGINMGEAAATIIITKEKGEIPVCITS
                     GATSNDANHISGPSRTGEELGVAIRKAMQASKVTVSEIDFINAHGTGTLFNDEMEANA
                     FSLTDFSNVPLNSLKGYYGHTLGAAGVLESAISVMSLLNNELIPSMGYETPGVTKPIN
                     VIRKKEMKPLKRFLKTASGFGGCNATLIFEKTE"
     gene            complement(38080..38562)
                     /locus_tag="VGA21_05045"
     CDS             complement(38080..38562)
                     /locus_tag="VGA21_05045"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEX9649904.1"
                     /translation="MGENILKQVSREESETLSDKVSLLKHTTQVRVRFNEADPLGIVW
                     HGHYIRFMEDGREEFGRKYGVSYLDFYNNGLVVPVVSVNCDFKSPLRYGDIMDVEVTY
                     HNTQSAKIYFEYLIRHSKKGNIVAKGSSTQVFVNHKTFDLHLTVPAFFEEWKKKMNLI
                     "
     gene            complement(38544..39248)
                     /locus_tag="VGA21_05050"
     CDS             complement(38544..39248)
                     /locus_tag="VGA21_05050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649905.1"
                     /translation="MKKYSSIAMVAMLVVLTYTATMAQSSADKPPPSSRGGATTSSKP
                     ELKLTKRDILNSSKITFLGIDLTETLFVGGYEEWKSEDELKRLNPQWNQLMVSEADKY
                     NLRRALRNNNIDYQVGICIDHNDNVNFKEKITFTDPGKPLTSEQIQNIMSSYDFKDLT
                     GLGMMFVIESFNKNKAEGTMALTFVNLNNKEIIYEERLSGPSAGFGLRNHWARSILEA
                     IIQIDKKRYKTWEKTF"
     gene            complement(39272..39706)
                     /locus_tag="VGA21_05055"
     CDS             complement(39272..39706)
                     /locus_tag="VGA21_05055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase"
                     /protein_id="HEX9649906.1"
                     /translation="MRNNPMLPKTDDILSLIPQREPMVMISRLIDANESCATTEFDIQ
                     PDNIFVVENQLTEPGIIENMAQTAAVQAGYYFSLNNIPVPIGYIAMVKNLEITNLPLI
                     NTHITTTINIRSRVLDITMVDAVVKIGNEEVAKCEMRIFIKK"
     gene            complement(39696..40574)
                     /locus_tag="VGA21_05060"
     CDS             complement(39696..40574)
                     /locus_tag="VGA21_05060"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX9649907.1"
                     /translation="MPQWQGKSKGNKSGYKIFVFICRKFGLFPAYFVLLFVAFYYFLF
                     SWESSKHIYRHFRNRFKFSVAKSILAIYRNYYIFGQVLIDKFVVMGDIKNNLTFTFDG
                     EQNLHEIVSLGRGGILLSGHVGNWEIAGDLLKRLKAKINIVVFDAEHRWIKEYANQIL
                     FANYNLIVMKEDLSHVYEIGEALLRNELICLHADRFVDEAKTLEMNFFNQKALFPVGP
                     FALAATFKVPVSFVFSFKETLRHYHFYGSPIIDYDSSLAKSDYIKYMATQFAELFEKM
                     VKKYPFQWFNYYKFEK"
     gene            complement(40577..40834)
                     /locus_tag="VGA21_05065"
     CDS             complement(40577..40834)
                     /locus_tag="VGA21_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009580243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEX9649908.1"
                     /translation="MDKNSIVEKINDFLAEEFEIEVSSITPDGSLAETLDLDSLDYID
                     LVVAIENNFGFKVKPEDFTSIRTFQDLYDYIFSRIDSKVDA"
     gene            complement(40835..42058)
                     /locus_tag="VGA21_05070"
     CDS             complement(40835..42058)
                     /locus_tag="VGA21_05070"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009580242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HEX9649909.1"
                     /translation="MHRVVITGLGIYSTIGLNRSEVRDSLCNGKSGIVLDQGRKETGY
                     RSGLTGKVEKPVLKGILDRRARIMLPEQGEYAYVATLEAMQQAGLEQEYFNKNEVGIL
                     YGNDSSAKAVIEAIDIIRTKKDTMMVGSGSVFQSLNSTVNMNLATIFKLKGINFTVSA
                     ACASSSHAIGIGFLYIKNGLQDMIICGGAQETNIYSMGNFDGLGVFSIREDNPAAASR
                     PFDKDRDGLVPSGGAATVIIESLDSAKRRGANILGEIIGYGFSSNGDHISNPNVEGPS
                     RSLQRALKDANINPSEIDYINAHATSTPVGDANEARAIDNVFGQHKPWVSSTKSMTGH
                     ECWMAGASEIVYSMIMMENDFIAPNINLETPDEFSAKLKIADKRINQNINVFLSNSFG
                     FGGTNSSLIVKKILN"
     gene            complement(42163..42891)
                     /gene="fabG"
                     /locus_tag="VGA21_05075"
     CDS             complement(42163..42891)
                     /gene="fabG"
                     /locus_tag="VGA21_05075"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019941217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HEX9649910.1"
                     /translation="MKYALVTGGSRGIGKAICVKLAEEGFLILINYKSNEIEAKKTLN
                     LVKEKGADGELLQFDVSKKEEVDEKLGGWIEANPGKEIEVLVNNAGIRQDTLLMWMKE
                     EQWNDVLGSSLNGFYYITHHIIKGMLSRKSGRIINIVSLSGISGMAGQVNYSAAKAGV
                     IGATKALAQECGRRGITVNAVAPGFIKTDMTKDLNEEELKRLIPMKRFGEPDEVADLV
                     VFLASKKAAYITGTVISVNGGLYT"
     gene            complement(42894..44432)
                     /locus_tag="VGA21_05080"
     CDS             complement(42894..44432)
                     /locus_tag="VGA21_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011585504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid ammonia-lyase"
                     /protein_id="HEX9649911.1"
                     /translation="MVKVGSRNISITDFQNILFDQELVELDQKAVQNVQANFDFLQSY
                     SKDKVIYGINTGFGPMAQYRVDDEHLQKLQYNLIRSHSSGSGAPFTEELALSTMLCRL
                     ISLMRGYSGINPQLVQLLTDLINKRIGACIFEHGGVGASGDLVQLAHLALNLIGEGEM
                     LYKGNIITTKDVFEIEGLQPIDVHIREGLALLNGTSAMTGVGVVNLIYAQRLLSRAIM
                     MSVITNELMQSYDDHFSKELNQVKNHEGQAKVAEIMRNLLSDSKLIRKRPDHLYNRKI
                     NESVFKEKVQEYYSLRCVPQILGPVYDTLIQAQRVLENEINSVNDNPIVDHKSGNVFH
                     GGNFHGDYISFEMDKIKIAVAKLSMLAERQLNYLLNNNLNQKLTPFVNLGALGLNFGI
                     QGMQFTATSNAAENQTLSYPMYLHSISTNNDNQDIVSMGCNAALLTRKVIDNSFEILS
                     VQIVALVQAIDLLQCHKSISSFTKAIYDDIRAIIPTFVEDEARYKELKAVKEYLMNRK
                     SLKF"
     gene            complement(44847..>45092)
                     /locus_tag="VGA21_05085"
     CDS             complement(44847..>45092)
                     /locus_tag="VGA21_05085"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX9649912.1"
                     /translation="IHLEWTNNLEKENVFKSSEALKEQFEKLGITPDKQIITYCRSGV
                     RAAHTGFILKDLLGYENVKIFDGSWIEWSHFGEEVEK"
BASE COUNT        13401 a         9172 c         8674 g        13845 t
ORIGIN      
        1 aattccagca ccgcatcggc tgcttgttca acttccatag taaaaggttg gttcatgcac
       61 acgaaagcta tatcaatatt tctcaatgcc cgcatttcag gaatgtcttc cgtgtctcct
      121 gaaatataaa ctttcttgcc tcctatcgtc agcacatatc cattccctct tccttttgta
      181 tgacgtgagt cttccgattc aggaaggttg tacataggga ttgctaaaat atcaaatcca
      241 acgattttaa cggactcacc attattaagg attttcaggt ttgttttaaa tttttcatcg
      301 agctgttctt caacggcttt tggaacaatg aaagaggctt tgctgacatc cagaatctcc
      361 agcgtagttt tattcatgtg atcgccatgt atatccgtaa tgataatgac atccggagcg
      421 ggtaattcat tatagaattc ttcaccgcca tatgggtcaa cataaattat caaattgtcc
      481 cacttcataa caaaagaacc atgctgaacg ggccggataa ttaaatctcc cttatcagta
      541 ttaattttat cgccttcaag ctcctgacca tacagactca ctgaagctaa acatataacg
      601 aatgttagaa aatttttcat ttttgaaata aagttgtgtt attcgggcta taaggtacaa
      661 attttctgaa agcaaattat atggagactt cggttaatat ttgtttgctt ttggtagtga
      721 tgattgacaa tcagaagatc gctttctcaa aatttctaca cacaaaagat aaacaaagtg
      781 gttaaaaaat cagaaattca gaaaatggaa tttgtcaaat ggagttttca aataaagata
      841 tatgaatata aagattgaag aagctgatga gcgacacatt tcgtccattg ctgatttgag
      901 taccgaactt ggctatccaa ttacccgggc aaaggtgact caaaatatga atataattaa
      961 ggagaagcca gatcacaagg tctgggtagc aaaggaaggg gagcaggtgc taggatggtg
     1021 tcacgcggca aaaacatgtt gggtaatgat ggagtctatg atagaagttt ttggactggt
     1081 ggtttctgca aaatcccgtg gcaaaggaat tggaaagatg ttaattaacc gggttgaatt
     1141 atttgccagg gaaaatggag ttgctgaagt gtggattcgg tctaaccgta aaagaagaga
     1201 atctcatccc ttctacttaa acctggcgta caccaacctg aaggatcagg cagtgtatgt
     1261 taaaaaagtt taaggattaa acagcgcgat tgatttcgaa agcgccgaca ggtaatcatt
     1321 aagatatttc ttcatttgct ttctcttata ctgcaggata taccgcggat gcgcaagtgg
     1381 aatcaattcc tgaaaaaaat tatactgctt gtttagagct gaaaagtatt tataattttt
     1441 gtcctctccc ataataatcg cggtgccgcg acaggctcca aaattaagtt gtgttttcat
     1501 tttttgaatg atgaaggttt caactgaatc ctgaagtttt ttattatcgt agtaattaat
     1561 attcctgcct tcctgagtaa acccaagcgg gcaaacggca gtaataaaga atgtattgta
     1621 aaattttgtg ctgcctccaa aagcgtttat aaaatcaaat acaaaaacag aagacaattc
     1681 atgcctttta tccaaatcgt tgctgatgcc acagtatttt tcaagattaa ttggatctgt
     1741 aaaaggaatt ccggttactc cggcaccgaa tcttccggga ttaataccca ggataaatat
     1801 tcgtgagtta ctgtcattat aaaacttatt atagaattgc tcacttaaag aacgaattgc
     1861 cggtttagag aatggatcaa ggattttaac tgaacccgga acattaaggt ccttactcaa
     1921 cgacttattg aaagcgataa ctttcttacc gaaagtcatc gatcgggaaa ttatcattac
     1981 ttcgagtatg gccttccata tctcaacatc accgaagatg cattcccacc aataatggtt
     2041 aaaatatata acctgtcgaa ttcgttctca tttattggtt cgcctggatc accgccaagt
     2101 aaccgtacca tatcgggcgt agtattacta tggccgctaa ccaggtagac tccgggcttt
     2161 gacttcattt cttcggcaat tacccccata tcctttactc cataaaaagt aaactccagc
     2221 ccaaacaatt ggcgaattag ctctactgtt tctttcgttc gattcagatc agtgcagtaa
     2281 atctttgtta cacctgcatt cttaaccagg cttgccagaa gctgagctct ttctttacca
     2341 atttcattta ggtttggatt tggaggatta tcagtataag gcgggggatt gacttcacca
     2401 tgcctaaaaa gtataacgac cgacttgcca ggcattccat caggatctcc cggttgtgcc
     2461 ggagcaccgg atgatactaa cagacaaatg aatgtaatca ggaggttttt aagcatagga
     2521 ttggtcataa gaggtgaaac actttttaaa ttccggagta attttaacaa ttaataacat
     2581 ctaataacaa cttttggcag taggttttgc ttcacagaag tgaactttga ataaaataaa
     2641 atattatcta tatgaaaaaa atatatctgt tcgccttatt tctgggtcag tccacccttt
     2701 ttgctcaaag tacccagggt actatcattt atgaagaaac cagacaactt caatttaatt
     2761 ttggtaatga tgcaagaagt gaagaaatga aatctatgat tccttcacag gtgtctactt
     2821 acaagcaact gctctttacc gctgatgcca gtattttttt gaaatacgaa aatctggaaa
     2881 aacagagtgc caatgaaatg aatttcacca gtgcttccgg aggaaatgtt gcgattaaaa
     2941 tgaaagaggc aaataacgaa ttcttcagga acatagctaa aggtaaaaca gtagagaaga
     3001 ttgaatttat gagcaaagac ttcataatct cggagccaaa agtggaattt gcgtggaaaa
     3061 ttaccggtga aataaaaacg attttggatt atacagtaat gaaggctacg atcgaagaca
     3121 ccgcaggtgt tattgaggcc tggtttacga ctcaaattcc cgtacctgca ggaccagaca
     3181 aatatggaaa tcttcccggt ttaattcttg agctttctct tgaaggagga aaaagaatat
     3241 ttacagcaac aaacatcaat ctcaataatg tggaagagaa acttattgtt gctccgaata
     3301 aagggaaaaa tgtttcacgc gaagaatttg aaaaaatccg tgcggagaaa atgaaagaaa
     3361 tggaggaaat gtatggaggt aagggaagaa caagaatgat aatcgaccac gagtaaaatg
     3421 aagcagggac taattatcat cctcttctgc ggatttttcc aggcaaaagg tcaggattcg
     3481 aacctggaat taactggagt ggtaaaagat tcatcaaaaa caaccttatc cgcagcgacc
     3541 gtaattctac tccaggctcg tgattcactg atgcattcat ttacagtaac aaataaggac
     3601 ggcgttttta cttttaaaag aattaaaccc gatgaataca tacttcagat aagttatatg
     3661 ggttatgcca cctatcataa gaaagtggaa gtaaattctg aaaatggaat ttttgatttt
     3721 ggtaacatca tcttgtcttc aaaatcgcag cttctggatg ctgtggatat ttatgctccg
     3781 gctataccaa tacaaattaa aaaagatacc attgaatata atgcgggctc ctttaaaact
     3841 aagcccaatg atttggtgga agacctttta aggaaactac caggtgtaga ggtcgatgcc
     3901 gatggaactg ttaaagccca gggtgaagaa gttaagaaag tgctggtgga tggaaaggaa
     3961 ttctttggaa atgatacgcg gatagccaca aaaaatcttc cggcagatgc tgttgataaa
     4021 gtccaggtct acaatgatct gtctgagatg tcaagattta ccggaattga tgacggggaa
     4081 cgggagaaaa ccataaacct gagcttgaaa gaaggacgca ataaaggata ttttggaaat
     4141 gttacagccg gatatggaac cgatgccagg tacaacttca agggaaatat aaatcgattc
     4201 agaaaaggaa tgcagctttc tgtaattgga aatttgaata atataaatca gcaatcatac
     4261 agcaataatg aattcctgag tatggctggc gggcttggag gaatgatggg tggcatgcgg
     4321 gttattcgga tgggaggaag tggatcgggc agcaactctt caaccgggat tgccacttcc
     4381 caaatggggg ggattaacct caatacagaa ttatcccaag acacaaaact ctacggcagt
     4441 tatttcttta accatattaa taatgaactt ctgaaaaata tttccagaac caatttcctt
     4501 gatgacgaat cttttgacag ctttcaggaa acccaaacta aaaccggcaa ttacaatcat
     4561 cgcctgaatt taaatctgaa cacgaagctg gataataccc aggatatctc tttcagaacg
     4621 aacatgtcat tcaacagggg caacagtgat aaatgggatg gatccctcac ctatgataca
     4681 atggaacaac tgagtaactc atctgcagct attaataatg cctcaggaga tgcaggaaat
     4741 gttaatgcca acttaattta ccggaagcga tttggtgatc ttggcagaaa ctttgttacc
     4801 aggcttcagg ttgacatggg cagtgatgat aaccggaatt tcctgacttc agaaacaaac
     4861 ttttatggtc cggtatttaa ttctgccaac ctgaaccaaa atcaaattgg caaatcagac
     4921 aaactggatt atggtggtca ggtttcatac actgaacccc tggggagaag aaactacctg
     4981 gaaataaatt atgaatatca gaattataca acagatgtcg gcaaagaatt ttatgacgta
     5041 gtagtaaatg atgaagtctt taatccaacg ctgagtacag gttataacag ggattactat
     5101 taccaccagg gtggaattac ctacatgcgc agccgggaaa actccaatat cagggcgcgc
     5161 cttgatgttc agaattcaaa actgaaaggt cagattgatg ataatgcgca actttccagg
     5221 ggattcttta atcttctgcc atcggcatct tatcgattat cactttcaaa ttcaagtaat
     5281 ctggatttga ggtattcaac caaccttcgc gaaccgtctc tggagcaatt gcaaccattg
     5341 gttgataatt ctgatcccct taacatttac cagggcaacc cggatttgaa accccaatat
     5401 agccacagag ccggccttca ttacagtatg tttgattcgt ttaactttat caacctgttt
     5461 ctgaatgtaa acggcactta cattcaaaac aagataacca atgctactta tgtagactct
     5521 ttattcagaa gggttactca gccattgaat gtggattatg attacaacat tatggctttt
     5581 ttgaattttg gtacgccatt gaaattcatt cgcagtaaaa taaacctttc aggtaattcg
     5641 atggcgagca aaagcattgt gtatgtgaat cagttggaaa ataatgcaaa caggtatatt
     5701 agtacagcga gtcttgcaat tgaaaacatc aaaaaagaac attacgattt cagggtaggt
     5761 acacggtgga cttttaatca aacccggtac tcagagaacg atgaattgga tcagaatttc
     5821 actaatcaga attatttttc agaactcagc atatatctca aaaagtggac ttttaatgca
     5881 gagttaaatt atatgcttta ccggggtgat gcttttcagg atgagcaggc cattccattg
     5941 ttgcatgctt acataagccg aacttttctg aagaatgatc gtggagaatt gcgtgcgtat
     6001 ggatttgatt tgttaaatca gaacaccgga atcaacagaa acagcgactt gaattatatt
     6061 caggaagaaa gaataaatgc gctcagccgc tattttctgt tttcattcac gtattcattg
     6121 cggggattag caggaggcgg taggggcaat tcaattaagt taaggcaggg aggctaacgc
     6181 ggttactttt ttggatcctg ccgcgaaccg aaaaatgtaa gaatggttat cttatcgttc
     6241 tttagtcggt agaaaactct tgtttgtttg gtaatttgga atccccttat tttcttgtct
     6301 cgtatttcaa gagagcccat ttcaggaaat tcctttagca aattcgtaaa ctcgtgggtc
     6361 ctgctcctga attgttgccg ggcagcttcc ccgaactcct cttcgatata gtgcaggatt
     6421 tctgccagtc tggttctggc cttctttgtc cagaagagtc tcatttgaga ctcttccaag
     6481 catcctcgtc attaattagg ctggaatcgt cttcggactc atcgtaagct tttaagactt
     6541 cctttttttg ttcttctgtt aacgaattcc acattctgct ttcttccgat ttttcacgtt
     6601 gacttagaaa gctgaagatg gctcgcagca gccgttcgtc ctcaatcctg tccactattt
     6661 tgtgtaaatt caattttaat tccgctgtcc ccatatttca atctttagaa tccctaaatg
     6721 taacattttt tttagtgcgg atatccctag gtgtctgccc cacaacagtt gagttccatc
     6781 ttttacgcta attctttggc ggagttatca ttatatgagc gccatgagtt ccacgatgca
     6841 tcaaccaggg atggccggga ccgtttggct taagcggtaa tccggttgat tcttccgtag
     6901 catagggaac atagattaca tatctatact gaacattgtt caatgtattg gcggctgcat
     6961 cataggtcac atctgtgccg ccatataaaa tatgtaatgt agccggcttg tcaggcattt
     7021 tcaattctcc agacttagct tctgcttcgc gagtgtcaaa tatttcctgc ccgcctttcc
     7081 cgtctgctct caattccctg ccccttgcca tgaatggttc cagatcctta tgataacaaa
     7141 cagtttcaaa attcgggctt ccgggatcat cagatcgaca gattaattca ttactgccct
     7201 cgcgaattat ttccaattca cccttctcat taaaacccat aactgttgca tcagcacgca
     7261 attcttcagg tccggccatt acagccattt tgatttgcaa ttctttatcg gtgggaattt
     7321 gcgcataaca aattgtagtg atggagatac atgttattga gaatgtaagt aattttttca
     7381 taccggtagc ctttagtcta atctaaaata gtgaatttta ccttaaagtt tcaaaagaat
     7441 cgttcctaaa gaaaactgat ggaagcagga aaaaatattc ttgtggtttc tataacagtt
     7501 atgaggcggg cactaaatat gaaatcggct tgctaaatca gattagactt caaaaagagg
     7561 taaaatttgc tgaagactca gcaaaggagt ttataccttc aatatatgca gaaacagaga
     7621 attatacttt caccagcaat ttaccggtac tttgccttga ctccataaag gcatgtgcat
     7681 caggtaagtg atcaaaatca aaaatcttac ctattaccgg tttaagattt ttttcttgta
     7741 caaatcttct gagctccatc tcaacttcaa ccgctacttt tgagttccca gaaaggtaac
     7801 caagatgcga acccgaaata ctataacttc gctgcaccat tttcagaatg tttacttttg
     7861 gcgcatgttt ccatgtaagc caccaggtat aggggttgaa tttcttaaaa ggaatacctg
     7921 catagccaat tacaactaat cttccaaaag ggttaagtaa gtccagactc ttcttaaaaa
     7981 catctccgcc aacaacttcc agtaccaggt caacaccctc cactttttcc ttaatgaatt
     8041 gataaaaatc aactttatta taattgatgg ggtgagcaac acccaggttc cggatcagtt
     8101 cgatcttgtg atcggatcca attgtaccaa aaatatcaca tccgaagcct tttgctatct
     8161 gcacagctgc agtacccact ccacccccgg ctgcttgtat taaaaccttg tcagctgatt
     8221 tgattttccc caatttcacg agggccaccc aggcagtgag gaaattaacc aggaatgcac
     8281 cattttcttc caaagcaaat tggctgaaaa ctgggaagac aagatgcatg tctgcacaaa
     8341 tcacttcggc ataacttccg aattgcgaac cgaaaattac cttctgcccg ggtttaacat
     8401 gttcaacttt agagcccact tcaataactt cacccatgcc ttccaatccg gggatataag
     8461 gtcgttttgg cgcccaccgg tatagtccgc gtcttgaaag aatttccgcg taattaattc
     8521 caataaacat cacgcggatt tttactttat ccggaagtat ttggggctcg ggtacttcat
     8581 gaattttaag aacctcaggt ttaccatgcc tggtaagaag ataagctttc atcgtattta
     8641 taagttcccc gaagctacaa cttagagcgc taaatcatta attatgtagt gcattacata
     8701 aaattatccg taaattttgt aattgaggac ataaaatctt gcaggaattg cgaaataaat
     8761 tacttattga ttgcatttgt ttgtaggata caaaaattcg aatcctcctc cggttatttg
     8821 gtttccttta ctaaaacttt ctcatttttt agaaaacgta tagatatact ttaatagcag
     8881 ggcgcggttt tccactcgcg gaacttccgg atcattattg tgggtatttt cattataaac
     8941 cacgaacaaa tccttgccat cattccaatt gtagcgcagc ttcagattta tacctgataa
     9001 atcctgagtc tgattccatt gaagaaatcc attaaaagac aaatgggtgt tcagagccag
     9061 cgtaattcta atccgggcaa tgttcccctc aaaatcgcgg ccatcaaact ctacccgatt
     9121 gtaaatcgcg tcaaaaccta aatcaaaata cttattgaca gtccaaatgg gacttaacat
     9181 tattgagtat cgatttccgc catagaattt accaccctca aggtttaaag caaatctgag
     9241 tctgtttccg tcaaacatcg tgtaattacc tgttacgcta tagaaagtat aatcaccggc
     9301 agtgatcacc acttcttcgg ataaattgaa aggttcactg aacgaatcat aattccggtt
     9361 gataaaaatc tccgaccgtg cacctgtctt tgctgtgaat ctaactcctg cagatgatga
     9421 cgtggtttcc agattatagt ttgaagagct gtgataatta ttatgaacaa tgaaaggagt
     9481 tatacttctg atttttgaac ttgtgaaaaa aaacccgtac tgtaacatca atttagaaaa
     9541 aactactccg cgccgcgcaa tgaaacctga cgaaggattg aaatctttac cgtagtatgc
     9601 aagctcaagc tgataaccca gtccgttgga atttctattc tcagacaata aataaccgaa
     9661 ggcacggttt ccgaattcat atgacgaagc caggtccgcc gattgcccaa cttttaaagt
     9721 aaggaagtta gattcaacaa gattaactgt ggcgtcaagg ccataagtta gttgagtact
     9781 tccatcatca tcaatcctgg aagaaaaaat tcctccaaca aatgagtttt tattgatggc
     9841 agactttctt atgcgcagaa caccaaaatt ctgtgatgga atggagtcgt cggccataat
     9901 ttgagcatcc atcagtccaa catcccagcc cttagcccgg gcagttaatc gtatgcctcc
     9961 gtacattcgc accggttttc cactgggatg aataccaatg acacggctat agaacaaccg
    10021 gtcattgcca actgttgcga aattaaatgt tccggatcgt tcctgaaaga attgtcgctt
    10081 ttccggaaag aagagtgaaa agcggctaag atttacctgc tgatcatctg cctcaatttg
    10141 ggcaaaatca gtattaatgc ttgcatcaag gttcacatta ttggaaattc cccatttcac
    10201 atctattccc gcttccttgc gaaatttttt attttgtgtt gtaacatccg gataagcatc
    10261 ataatccatc tggtaaccgc ccaataaata aggagtgaca tataatggac tcttctttct
    10321 aattccttcc atcagaattg tttgagcctg gctggctttc cacgcacctg aaacccaatc
    10381 aggcctgata gcagggaaaa catggcgctc attttttcgg cctattaacc tgtgaacaat
    10441 aattccaaat tgaacctggc catccttatc ctcaaactta agagaagtaa aaggaatgcg
    10501 catctctgca aaccatccct tttcatttcg ggtaacttta ccctcccaaa atgaattcca
    10561 gttaacgttg taaaataatg caaaatcacc ctcagcatca ttattgattt ccgtatctat
    10621 tttgctgcct gagggagtta ggctgaatcc taaaacagtt tgattgtcgt catacgagtc
    10681 tatcaggaaa ttgaaaaagt ctcccccctt gtcaacgtcc cgaatcatgc tgttgccaag
    10741 tatttttgat ggctgcgaat catagaacct gcctgatgca tagatgtagt tgttgtcata
    10801 ggcaacacgg atttcagttt tctctgtcat ttctccggca aaaacagggg tgtacatggt
    10861 taccggaagg ggatcaattt gttcccacgc ggtttcaaat ggatttccgt caatttcaac
    10921 cagtaattgt acccggttaa ttttcacaac attctgtgct tgcaaactca cagtaagcaa
    10981 agagaaagcg gcaatcagaa ttcgactttt cattccagcg tcataaaaaa ttgttacaat
    11041 attcatgatt ttgtcgctga ccatactatt aatcatatga atagttttta atttcattat
    11101 taaaattctt aatattaaaa ataagctgtt ccttataaaa ttctagtcaa ataattcaaa
    11161 tgcaaaagag ggattaatag ccaattatca gattgagttc tgaagcaatg acacaaaatc
    11221 atgttgcaag agactgattt tgatcatttt ttccgacccg gaaattgttg atcttatttg
    11281 gaaagaaaga gaaatgaaac cgatgacaga aaaattcctc ttttacctgg gttacgtagt
    11341 aacaatatta gccgtgttag gggcgacgtt ttttatctgg agtcttggtg tctacacgat
    11401 gctaaaaatt ctgctgcttt tgcctttgat ttatattagt gtgctcctga ctttatccct
    11461 ggaagatggt tttaaaaaaa gcagatggag ttaattgaat ttttgtgcgt acctgcttcg
    11521 ttcaagaaac tcgtgaaagt cccggatagt aaggatatcg tgaatggctt gtttcttatc
    11581 aggaatttga tcatagtaag atttactgat tttatagccc atccaataac ctaggtcgtt
    11641 aggtcttccc tccattgaac tatacaacca tcctgtaaaa tccttaccat ccattttttc
    11701 tttgaattct ttaaataatt ccttttccct gggatttgca aaagtgtgag caaatttatt
    11761 gatgtgttta ccggaaatta attccgcgag caaatccgcg gaaccttctt tgatgctctg
    11821 ttcaaggagc gttcgggacc ggtatttttg ctggtaatga ataagttcat gggcaactat
    11881 gtgtggtaca tggctcatcg gcttaattac agttaccaac caatcattta gttcttcctt
    11941 tggtgtatca ggtctgagtc catgcatttc agcaccgata atcaaacctt gcctggaggt
    12001 tgtgccaccg gagttcatag cgccgataac aaaataaact ggagggaaca ccgcatccgg
    12061 atacaattct ttcatattct taacgctttc cgcaatttca tttttgaatt gcaatatgct
    12121 atcagtactg ggtttaaggg aagcaaaata cttctcgtga cttttaacca cttttgccag
    12181 atgttcggca tttcgaattc tattatccat gaatccttct actcccttag agccgggatc
    12241 taaatacaat ttttgaaata cttccgggtt aaattcaggc tttgccgcct caaaggcatt
    12301 ccagaaatta tctatatccg aagtaattat ttccatggct gccggatcgc ggttcatttg
    12361 cgccgaacta caaccggcaa caaacagaaa taaaaacaaa tgtttcatga cctctgaatt
    12421 ttggtggaat ttaaataaag atcgtgaaat ttagtttgtg gtggcgcgaa accatgatgg
    12481 acaatctttg ttaagttcta caatcgaggc ttttcccaac taaaccgctt atttttatca
    12541 ccggaatgaa tctgccgaaa tatttcctga atcttctcct tggtctttgt gctctgggtc
    12601 aatcaatggc acaaacattt atcgtggcag gaattattaa gaatgaagaa aatggcgagt
    12661 cgctgcctta cgcaactgtg aaaataaagg aaaccggtaa gggtgccgta actaacgttg
    12721 agggttactt tactttgctc aatctgccgg gagattctat tacaatcgaa gcttcttaca
    12781 tcgggttcta ttccagggag tttagaatat tgccactgaa tcagaatttt ttagcaataa
    12841 cattagcacc atccaccgcg caactggaag aactggttgt aactgcagaa agctaccaaa
    12901 ttatgaatgc atctgaagga attagctttg ttaaactttc accaaaagat ctgtcactgc
    12961 tgcctggtat cggagaggaa gatatttttc gttctttgca attaatgcca ggtgtgggtg
    13021 ctaccaacga aaattcaagc ggcctgtatg ttcgcggtgg cacacccgat caaactcttg
    13081 tcacctatga tggaatgacg gtttatcatg ttgatcattt ctttggattt ttttccgctt
    13141 tcaatccgga tgcaatcaaa gatgtacagt tttaccgggg agcttttccc gcaaagtatg
    13201 gcggcaggac atcgggcgtt gttgacttga caggacgaac aggtagcgta gataaaattg
    13261 aacttgacgc agggattaat ttgctgagtt cacgcctcac tgtaagttta cctttatttg
    13321 gaaaggcttc tatattgctt tcagggagaa gatcttattc ggattttttg gagagtggct
    13381 tatacaaaaa tatttatggg ttgattgatt ccggaggcca ggaccaggat gcggcgcttg
    13441 ataatctgga tccggatttt actcctacat cgcatttcta cgatttgaac ggtaaggtta
    13501 cctatcggcc gggcattaat gataatttca ctcttacttt gtataatggg gaagatcatc
    13561 ttgataaatc cagagatata aataatgtca ttactatacc taacctatca ataagaaggc
    13621 ttcttgaaat tgatacagaa gatcttacgg actggggcaa taatggagta agccttcgat
    13681 gggcacggca atggcatccc aaatttttta gcaatgccct ggttacctac tctacctatt
    13741 tcagtgattt taattttgat ttcacattca gattaagtga ttttgatacg gacactactt
    13801 taattctggc cggctttaag gcttttgaaa aaaataaggt gaaagatttc acctacggcc
    13861 tggaaaatga atttcactta tcagaaaatc ataagctgga ttttggcttc caggcgaaga
    13921 actacaatct cacttaccaa tttgtcagag atgatacaat taccatatat gacagccgtc
    13981 aaaagtcaga tgaaattggc atgtttcttc aggaaacctg gaagattggg aaatcaattc
    14041 agataaatgg aggaattcgg ggcatttatt atgatttgct ggaggagtat ttcctggaac
    14101 cccgaatttc ttttgaattt agttttctga agaactggaa gataaaagga ggctggggta
    14161 ttttccacca gtttgtaaac agggtaatca atgagaatgt tactacgggt tcgcgtgatt
    14221 ttttcctgct gtcggataat caagcactgg atgtgcatcg aagtgagcaa tttattatcg
    14281 gcggaagttt tgagaaagaa aactggctgc tggatattga attgtatcag aaagaaatga
    14341 ccggcttatc ggaattttca ctcaggtttc agcgacagaa tgaaattgat ccaacccaac
    14401 ttttttttaa aggaaaagga aaggcaagag gtggggaatt tttgttgcag aagaaatcgg
    14461 gcaactacag gggatgggca agttattcta tttccagaat tgaacacact tttccggact
    14521 tgaatgcagg aaattctttt ccagcattac atgaccagtc acatgagttt aagatggcgc
    14581 atatttacag caccggcgac ctcaatctcg gactaacttt tatttacggt tccggtaagc
    14641 cgtatactca accggaatcc ttatatgaga tatcgttgct tgacggtcgt gatctgaatt
    14701 acattcacgt aggcacaaaa aacgcgaacc ggctgcctgc ctaccacagg ttagatgttt
    14761 cagcccatta cacacttaaa ataaacaaaa gtaatttggt gctgggcgtg agtatcttca
    14821 atttatacaa ccataaaaac gtctggtaca aacagtttga tgctaccgat ctcccaatac
    14881 ttattaccga tgtgaattac ctgggcttta cgcctaattt ttccatatca tttaagttat
    14941 gatgaaaaag gattgtctaa ccatagtaag tttactggtt gttttgcagg catgcaattt
    15001 tgatgatgat ccattgcctg tggagcaatt ggtggttgaa gcttttttat atgctcacga
    15061 accggtagat gatattcaca tctttaccag tattccaata acgacagata ctgctatctt
    15121 tcctccaata agtaatgccc aggtcaggct gatcagggat gaagtaattt acgatttgat
    15181 tccttcggct gaggcagggt attatcacta cccggatact gatttggatg ttaaagaagg
    15241 ggacgaattt ctattggaaa tttattatga taatcgcgca attactgcta caactgtagt
    15301 accgccggct ccgctcaatg taaaaattaa tcacgatacc atagagatac ctcctcttgt
    15361 tccgggagtt attgctgaac tcgcggaaat ccaactgaat attacctggg aaaatcccgg
    15421 ggaagatctg catttcgcgg tgatagaaaa tattgagccg ttggatgaat tggaattaat
    15481 catcccgcag caatttcagc ctgtacgtga agcattttta attattgccg aacccacgct
    15541 tgacactttg cagaatttca atgtttcctc actgatttac ctgggcacgc atgtagcgaa
    15601 agtatacagg gtgaatcagg agtatgccga tttatattca agccgtgccc aggattcccg
    15661 cgatcttaat gagccaccga ccaacgttgt gaatggcctg ggaatttttt ccgctttcag
    15721 cagcgaacag gatgtgtttg aagtgaggcg gaggtgaggt gattcaagcg aactttacat
    15781 gctttttttc gctaatgcat gcttttatta atcacgtttg taaattcaac catgcatttc
    15841 cagctttacc gcctttccag atactttttc cagttggcgc tgcgattttt ctttagaaga
    15901 attgaggtga aaggtctgga acgggtcccc atggagggtc cggttattat cgtagccaat
    15961 catgccgcat cattcctgga tcccattgtg attgccgcga aactcaaagt tcctgtttat
    16021 tttattgcca aggcacgggc tctgaaacgg ccggtaccta cctggttttt gaaattgttt
    16081 aatgtgatcc cggtttaccg ttctcaggat gatccaaccc tgatggataa aaatcagact
    16141 agctttgaag cttgttataa acatctggaa atgcgaggga atatcctgat tttccctgaa
    16201 gggatttcac attcaatccg gcatctgctg aaaataaaga cgggagcagc ccggatggct
    16261 ttgggtgccg aagcccggaa caagttcaac cttggattaa aaataattgc atttgggatg
    16321 aattattcca atcaggaaaa atttcaatcc aggttattcg tatcggtgag tgagccgata
    16381 aatgtgattg aattcaaaga tgtttacctt caggaggaag tagaaggagt gaaccggtta
    16441 accgaaaaaa tcgagaacag catcaaccaa agtattatta acgccggtga tgagcgtata
    16501 gatacactca tcaacaggat tgaacggtta atgaaagcca ggattctgaa agactggggt
    16561 gtcaaaaaag aaatacttga acaggatcat aatgttacca aaagaattat gcaggcgatg
    16621 gattattttc tggaaagaga tcaggcacgt ttttctaaaa tggaaaatga aattaatcaa
    16681 tatttcgaat tgctgaaaga cgtggatctg cccgaaacta ttttcaaaag taaaaaactg
    16741 actccacaag cggtattgtt ctatatcagt tactttatcc ctgggcttct ttttttcatt
    16801 ccggggttgg ttggcaattt tattccttat aaactctcta tactaagcgc cagggggtgg
    16861 tcaagaaaca aggagtacag gggtgcagca ggttttttag ccggaatcct gatttttatt
    16921 tttttttatt gtttggaatt tatgatgtta aaggacttca ctaaaagtct ttggattcct
    16981 atcgtatcaa ttattgctat gccgtttttg ggtattttta ctttttatta ctggcgaaag
    17041 ctaagccgct tttattgcaa aattattttc accttttcta aaagacaaat atttatccgt
    17101 cgattacttg ccagacgtaa ttccattatt cgtgaactgg aattcgctcg gcaggagttt
    17161 ttagaggcgg agagaaaccg gctggctttg tgatgcccgg atcgacttaa gccaggggag
    17221 agagttttga agaaatttta tgcataaatt gtcatgaaaa atgacttcaa ttcgtcatca
    17281 gttattcaat tgcacattta aatttattga caactaaaat attagaaaac atgaaaaagt
    17341 tattcttgtt gctctttagc atggcagttg ttcagatcac ttacgcgcag gaaaaacctg
    17401 aggaagagaa agaccaatct tttatcctta tctattcgcc gggagaatta tgggactctt
    17461 cgaaatcacc ttcagagcaa cagtattttg cagaacattc tgctctttta cgagacctca
    17521 gaaacaaaga aagaattacc attggcggac ggtacagcga caaaggactg ataattttaa
    17581 aatcaaaaga tgaaactgag gcaaaagaaa ttgcggaatc tgatgtttcg gttcagaaca
    17641 aattgtttaa agttgagata ttcctcctaa atgcatttta taaaggctgc attgattaat
    17701 tcaattagca attaatccag cggttcactt tgctgcatct cttccttaaa gacagccggg
    17761 agttagcaga aaaaaggatg agcttgttat taaggccaaa gccagccaaa tgtgccggca
    17821 gtaaccagcg catacagaag cccgtcaaaa acagatttaa gagttgatga ccaggatctg
    17881 ccgtaccaga tggaagtttg aagtaaacca aggctgtaac cggcaaaagc tgttgtgcct
    17941 gcaaaccgga ataccgcaag gtaatcattg ccgggttcca gtgccctgcc ggcaacgtac
    18001 gcggcaaaga ttccaactat aaccgagtat aaaaaccact ggatcaggtt ggaggccatg
    18061 ccttgcattc cattcgggcg aactgtcata aaaattaccg gacctttctc tacctttgcc
    18121 ttgaattcct cgcttttcat ttccgcggaa ttaaaagccc tcggaacaac ataatctccc
    18181 ggcggaatat tgagcggacg caaagcatcc atcatttcat cttccttcgg aacttttcca
    18241 aagtcggaac gatgataagg cagaaccata tgaatgatcg aactcagaat aaatacaaat
    18301 gcggctgata agagaatggg aagccaaagg tcaattattg ataccatggt tgtttatgtt
    18361 aagttgatgg aaatgttaac ttagtaaatg tcaaggattt atgcaacaat tcatttagaa
    18421 tgatgctcct gacaataagg tttagcaaac gaagtactca atcaggcgat tcgcgtccct
    18481 caacgttttg cttaaaaccc acttgaagca ggtgattttc ggcaactttt tatctgagaa
    18541 atccatgttc tgactcatgc atcactttat aaaaattatt atattgcctg tccaactaac
    18601 tatcatacca agatgaaacg attactaacc atactatttg tttttgccgt tacattttct
    18661 ttttcgcaga aaatgaaagt gattcaggga gacctcaagg tgttgaaagg tcaaacttct
    18721 attaatgtag aatatgatta cgaagaccag gcggtaggta aatacgaccg tgagtcggat
    18781 tacattgctg aaagaaaagc aaaatataat gctgatgagg ctggtcgtgg agaccattgg
    18841 gccgaagaat gggttaacga tcggggaaac ctgtatcaac ctaaatttga agaattactt
    18901 actgacagaa ccggttggaa aatcagccag gatgcacaat atactatcat tctaaaaact
    18961 ttgaagactg agcctggatt taatgtaggt ataatgcgac aaccagcttt cattgattct
    19021 gaagctatta tcgtggaaac ggccaacaag gagaacattg tttgccgaat cagcatgcta
    19081 aaagccccag gacaggatgc gatgggttat gattattcca caggaccaag aattgcggaa
    19141 gcttatgcca agaccggaaa gagtctgggt gctatgattc agaaatcgac taaataacct
    19201 gccagcgagg cgtcagaatg gttttactgc caggatcatt tttctctgga aaatgtctgc
    19261 tgcaaaccat tctgccctga cgcccggctt cttatttccg gttatccagg aatcgtttta
    19321 ctttcctgct gttgccacga aaatggatct cgaacaattt attctgagag gtaaggatga
    19381 tctccggtac aacccgtaac cgggatttaa aaatcgattt tagtttttgt tcaaattcat
    19441 ttttgtttga tgaatctgcc tctatatgga tgcgtaattc atcagttcct aattcatttg
    19501 tgattacctc aaccgcatag tctttcacaa aatcaacttc actcataata ttaaatattc
    19561 cgggtgggta aatagttgtt cctttcagct ttatcatctc ttgttttctc ccaataaccg
    19621 gacctagtct tagcgtattt cttccacacg aacatgattc atcataggcg acagagatgt
    19681 ctccggtttt gtatcgtaac aaaggcattg cctcaacacc cagggtggta attgtaactt
    19741 cacccgggct tcctttttct accggcatgt tattgtcatc cagtaattca acgataataa
    19801 gttcagggtg aagatgtcct ccggtgccgg cagaacactc agtgaatgcg gtttgcattt
    19861 cggtagaagc atacgtatta tacaacttga tgttccagcc atccaggatt ttctttgcca
    19921 gaatacttaa acccagttcc ggactgcgaa tactttcgcc tatgcaaacg gcgcttctta
    19981 cagacgtttg atttagatcg atattttctt ttccggcaaa ttcaataagt tttaccagga
    20041 atgatggaac tgctaccagc acagtagggt tcaggctctg aatggactca agctgcaatt
    20101 gcggcaaacc cggaccaacc cgaacaactg tggcgcccaa ttttcgaatg ccttcgtagt
    20161 aagctattcc tgccatgaat tgccggtcga gtgtaagcat gagctgatag caatcttttg
    20221 cggtaccatt cgcacagcag aaagaaatgt actcattgta tgccagccgt tccaaatctt
    20281 tgcgcgataa ggcgatggta accggattgc cgagagttcc tgatgttgcg gtgaattccg
    20341 caactgcgtt tttttcaata cacaaaaatt cccaattatt attttgaatg tcatcttttg
    20401 tagttaccgg aagtttttga agatcttcca gggacttgat ctgactggcg tcaatttgat
    20461 gttcgatgaa aagctttttg taataaggag aatgtgtaat cagatattgc aagagttcac
    20521 gaagcctttc ctcctgaaac tttttgattt ccaaggttga tttcttttca atttcaggaa
    20581 taaaactcat ttctcaagta ttactatggc agaagcgact gtatccgtat gggttaaaga
    20641 tacatgaact tttttccatt ttccttccag ctttttaaac tctccggaaa gcaaaatttc
    20701 aggtcgtcca ttttctcggt gattgacttg aacctggttc aattcataac taattcccaa
    20761 tccggagcca agtgctttaa gaaatgcttc tttaactgca aaccttgcgg caaaatgttc
    20821 cgcaggatga gtattctttt cgcaatactc tatctcattt gcagagaaca ctttcaactt
    20881 aaattcttcc ttgcggattt tttcggcaat ccgcgacacc ataaccgaat caataccaat
    20941 tcctgcaatc attgaagctt ttctttacag attttaatct gattcatcat gtaatttgct
    21001 tcctgagtgg atgacacttc tttacccagg cccagttcat agaatttctg agctttggca
    21061 tattcttttt ctttgaaaaa atgatcaccg ccaagcatgt atgaaaggta ggaattggtg
    21121 ttccattgaa caaggctgtc aggattcaaa tttgaacgat ccgaaaaggg aaacctgaat
    21181 tggacaaatt tctggtagtt tttgaactca agtgaataaa ggaatggatc ctcggaaata
    21241 gtaaatgtgc tgtcggaaat ttcaacatta tcggttttgg gtgaagaaga aaaaacctta
    21301 ttcaagtcgt agcaaataaa ttttcccaat tgatatgggg aagttgaaat ccatacctta
    21361 agcctctctg gctgaaaaat gatcgcgtgg tgtgaaatca gttgattgat tgctttttca
    21421 tttccaagac caatgtcttt tccacccaga cccaattggt ttcttaaaat ttccgcggca
    21481 tctgtaacgc tgattttttc tttttgattc agaagttctt ccatgcggtt gtgcctgtaa
    21541 ggactgggac tggtcttcat gtgcttcagg tttaattcag tattcgacaa taactcgctt
    21601 tgaaaatgat ttgcacatat tatataatct ttgccggact cataaaaacc ggtttcactt
    21661 tctgtctttt ctattacagc cgcttttccg tcaaaagcgg aaccaaccag gaatgattct
    21721 gagacgaaaa cctttctttg ctcagcaata tggcgtgcct ctgaaatatt ttttgcatac
    21781 tgaagaattt cgcgggctac gattgaaacc ggagtggcgg aacctgaggg tattgctgat
    21841 ttagctgcat taatcgttac agtcagtccc tgatcattca ttccgctcaa aactccggtc
    21901 atcccgcccc aggtcaccat cataaactta tgtccctgat ccggattgta gaaagcaata
    21961 attttatctc tcgcgaaatc ctggccaacg tagaagtcaa aatttctgcc gattagtagt
    22021 gtagagtcag cgctcttcgt accccatact ccaaaagaag tacagccgac aaatgacata
    22081 ttttgcagag catggccaat atcatgagcc gcgtggtaat tcagcagacg gtgataggga
    22141 ggggcgatgt catcgaattc atgggaggcg ctttctgaaa ctccgaatat ttccattttg
    22201 tattcaggca aaatctggtc ttcaagattt ctgttaaacc agccaacaaa atatttcaga
    22261 aattccaagt aaagggtatt gggaacaagc cgatgtattt gttgggtaaa aacctcctcc
    22321 tggtaatgta ccagttcccg ggtaagtttt ccaatgatta ctcctctttc aaaaggattt
    22381 ccttccacat acaactcata aagaccagat tgactctgac gaaaccagct attatggata
    22441 gtcatcaaac catccggtgt tttaacgggg tttaattcaa ggctggagag atcatcagga
    22501 acaggaggct gcaccgttgc caccacatac aggtaaataa acaggatcag aagcagtgag
    22561 aatattccac ctgcaattcc aagcaggaca cgcaaatatt tgtttttagg catttcttat
    22621 tttatctaac aacgcgggag tgtcaattaa ttcaccgcag gtgactattg cactcatggt
    22681 aacgcccaaa actccgtgaa ggtttagatt ttggccggta agaaacaaat ttggaatttt
    22741 ggttcttgga gagatgaaag ttttcatagg ttcgcgaaaa tcttttttaa ctccgtataa
    22801 ggtgccatca tacgtaccca cgtaatcacg cagagttaaa ggcgtagcac agtaataact
    22861 atctattgca tctttaaagc cgggaaatct tctctcaaca acatcaaata aaatttcagc
    22921 ttttcgcttt ttgaattcat cgtaaccttc accacgatgg gattctttgg aaacaatatt
    22981 ataagagtct gcccattcct gtacatcttc aagatgcatg taggccatta atgaaattcc
    23041 atcggcatag ccattggatt ttgaagaatt gctgaagaac attgcataac ccaatggcca
    23101 gttttcttct ttgtaattta ctatttccca aacatcatca ttttcgaaat aataaacatt
    23161 gtaatttcta tatggaaaag cattcttttt cattaccaca ttaacaagga atgtagacat
    23221 tgtattttca agcttttcaa tgcgatgccg gtaggccggt tttagcaagc gactttctgt
    23281 taattccagg gttttgcctg gatgaagatt agaaatgaag gttttcccaa aaactttgtc
    23341 tcctttttcg gttaacgcgt atttgatttc tcctttttct tctattaatc gggtaaccct
    23401 ggtcttgcga aaaatttgcc cgcccatttt tataatctct ttagcaagaa atcttccaat
    23461 ttgagaacct ccatcaatga agcgatatga actctcaata taactgttga tgatcagagc
    23521 gtgaatgtaa aagggagttt tgtaagcctc gccggcgtaa agaagattgg tgctggctaa
    23581 aacattttga agtttggcgt tgttggtgaa agattcaata atggttttag tatcaatttc
    23641 cagtgatgta attttttcaa aaattccgcc tgtacgtaaa ttgtacaaag gaaatttctt
    23701 gcacgtttct ttaacagcgt tgcagtaggc aataattccc gctctttctt cagggaaatc
    23761 ttcagagagc cggttaataa aattctcata accttgtgga tgccggaatt ctttttcatc
    23821 attctcaaaa ataataacgt catatccatc ctcatccatt ttttggattt tcaccttatc
    23881 cattatgccc aggtatttga atatttgata aagattctga cctggctcaa gaccgcccac
    23941 ataatgaacc ccggaatcaa agattacttt ctttcttaca aaggtttgca gggtacctcc
    24001 tatctgctga ttcttctcaa gaatgcaaac tttgaaacct tcctttgcga gaatggttcc
    24061 gcaaaccaaa cctcccatgc cactgccaat tattacaaca tcgtattgcg tatccattac
    24121 ttcattctta gcacaaaaat aacattagag gtgaattttg catcatcaat aacttcaatt
    24181 tcaagattat gcttagcagc ctccgctctt atcttttctc ctgtcatgaa gtttaatgat
    24241 tgggttgact tattaaactt cattatctta acagaaaaga attctgaaag tttggttccc
    24301 ctgtatctct ctcctgcatc ggtaatgcct tcgcgaagaa taatcattcc ttcgggattt
    24361 aatgcctgaa cgctcttttg aattactttt tcctgctggt ccggattaag ataatgtaat
    24421 acatcgctca aaataataac atcagatttt tccagttcat gctccacgat atccgcgtaa
    24481 acaaaattta gcttttctgt tctggaatag caatttttag ctgtttcaat tttatcttca
    24541 tcataatcta taccggtaat ggttctttct tcagataaaa aatgcagcat gtagcataaa
    24601 aatccatatc cacaacccag atcagtgatg ctggatttct ttggaacaat gctttcaaac
    24661 ggttgataat ttttttccag cctgagctta attttcaaat accactccag caccggccct
    24721 ttgaaaagat agttagatat aagtttatat ctgaaatatc ccggtgtttc atactcagct
    24781 cgcattgcct ggaattcact tttaaaatac ttacttattt tcctggcacg ctccttgtat
    24841 ccggttccga aatcagaatc atccggataa attttcggga ggaactttaa tgtgtaggaa
    24901 gagtcctgca aataaaaatc acctttttga atggtagtat tcattccgtg tataacgaga
    24961 ggagtaattg gaattttaag atgttcagaa aggtaaaatg ctcccttatg aaagcggtga
    25021 atttttccgt cgtatgatct ggtaccttcc ggaaagatta ttaccgagta cccttctgct
    25081 aaccgtgctt tcagtttttc aattccttct tcagtccccc acataaccgg ataatagtcg
    25141 gcaaatctga ccactctccc aaagaccgga gagttccaaa cccatttgtt ggtgagcagg
    25201 atcaatttgg gatgaagcat agctgttgaa atgatgtcac agaaagaaga atgattcgca
    25261 attattatgg aggcttcctt aaaatcatga ttgttccgca aaactctttt ttttacatta
    25321 accattatat aaacaagcga gactgtagcc aatcgtatta atacatgata taaatattta
    25381 agtttctttc ttccaatggg aattaaattg gtaataaagc caattatagt gagaagtagc
    25441 gcaccaaaaa cgtaataaga gaaagcaaat aaagttttta ataagccaaa aaaagtcatt
    25501 ggcggaaact tttttccggc tctgttggca atgaaaaacc taaacaaaaa tggctcaatg
    25561 gtttgcgaca ttatcacaac gcagacaatc ccgataatag aaataagcgc gatggactta
    25621 agcgccggat gctgagcaaa tatcagcact cctaatcctg aaattgtggt taaggcagaa
    25681 agaattactg aagtgcgaat ggagataagg ttgtttttgt tactccggta tttttgttga
    25741 aggccatcca taatgaagat gctataatca tctccaaggc caaaaatgaa ggtagaaatc
    25801 ataacattca caatattgaa ttcaatattc aataaggcca ttattcctaa aatccaaatc
    25861 caggtgatta acatgggaat gaatgtaatg agggtaagtt caattcttcc gtaggagacc
    25921 agcaatgcaa agaaaaccaa caaggaagtg aaagtcagaa tgaagttaaa gtcttcatta
    25981 acatattcaa ccaacattgt agtgagaatt tcgttatcca cggccaccac tttattcatt
    26041 cctgccagcg cgtcataaag tttgtttttc tcttgctgat ccacctgaac aaaagttatg
    26101 atggataccc ggcccggttg ttctgcaaaa ttatcagcga agaaagtggt ctgcatttcg
    26161 tcggttttcc ggttgtccag ggttgtatac ctgcgctgca ataaatcaaa tgccggctgg
    26221 aacgcctctg cacgaaatcc aagttttgag ctttcttgtt ttattaaatt gactacgctt
    26281 gcacgcttat tttcatccca ataattattc caccggttaa ttctgctttt ttgtaaggaa
    26341 tctgagagaa tgaaatttga gaccgaagat atttttttaa ccagcccgct gttttgaaga
    26401 tttttaagca ccgggaacaa ttgttcattc ttttgtaatg catcttcccg ggtatttccc
    26461 tctgttacca gaaaaactga tcgaaaggca aattgattta tttcgttcag ttccttctgc
    26521 gcctgcttca aatcgtcaga catgaagttt aacttgttca tatctgaatt aaacttgacc
    26581 tggccggcgt aataaaagaa aagtggagtc agtataaaaa tcagggctac aatgtaattg
    26641 gaatgttttg tatcccggaa gccaagcttt tcgattcctt tctcaacaat tcccggttct
    26701 ttcctaaata aagtggccgg gaaaaagtgc gggaggaaga taagcgagaa tagcatggcg
    26761 ccaatcaaac tgaagccggc aaaaagtcca acatctttga gcaacgacgc atttacaaat
    26821 tggagcccca ggaaagctaa tacggtagtg gcgcttccaa cggtcatggg tttagcgagg
    26881 tcccggatgg tgtcgcgaat atttgccgta tgtcgaaggt gtacaaaata gtgcaggctg
    26941 tagtttactg caatccccat tacaatggag cctgctgcaa tcgcgattac tgaaaccgaa
    27001 cctttgatta atgaaataag ggccagggaa aataatgctc cgaaaaccac agggaaaagg
    27061 atagcaaccg tgactcgctt tcttcgaaaa aatcctacaa gaaatatcag caacattacc
    27121 accataatgc tgacagtaag agccgtatct ttacgcaatt gcaaagcatt tccaacagat
    27181 accgccggag aaccaaaata ggttgcatta attgctggaa atttttccgt aattctcccg
    27241 atttgttgat ccaggccatt aatcaattca agattatgac ctgtatcatt aggcgggtac
    27301 ctggtagtga tgaaaaatac aagatgttta taatctgaag ttatgatata attatcgtac
    27361 agggtgtagg tctcatcata ttgaagttgc gagagtttag ataatattgg cctggagatt
    27421 cccaaaggat ctttcagaat aatttgccgc gatacaactc cggaaggtgt gatgagttgt
    27481 ttgtaattgg attgcattga gttagcaatg ctttcaggcg tagttagtga atccaggatc
    27541 tggtaatcgt tttcatcaag aaacacaggg agattatcaa tcagataatc gaagatctcc
    27601 atgacaacct gatcgtctac cttgtatttt aacgctgaat aatattctga gagagattgc
    27661 tgtatacctg caaccaattc ttccgtaaaa agaatcaggc tgtccggact tggcgctgcc
    27721 agagaatctt taagtgaaac cgtaaaaacc attttctcca gaaagttaga accttccaaa
    27781 acctggttta atttctcaac ttttctatcg tttgggaata ctctggagat atcttcttca
    27841 atagtaattt gtgaacccag tcccaggata acaagtaaaa caatactaaa aacaacgaag
    27901 aaggtttttc tgcgtttctg aaaaaaatca taaagcccaa cgaagaagta ttccatttaa
    27961 gaattagcgt ttgaaaccac tctcttcttc ttgccaaaaa ccttaagggt aaaataagta
    28021 acaaaaccga agaaaagtcc gcaaattgtt gccagggtaa agctgccgag aacatattgg
    28081 ataaaatttg attgaattga atccagggtt aattccttgc taaacgcaag ttgatcggcc
    28141 tgctcgccca tccagatttt accggttatg taactcaggt acaatattat tggaatcatg
    28201 ggaggtatgc taatgttggc ggcgactatt accagggctt tattgagacg cattacaatt
    28261 gccaatgcca atgccactaa catttggaat ccccagatgg gcgcgatacc cataaaaatc
    28321 ccgaatccaa tgctgatagc tttcgtcctt tcggtttcat gagggctggc aagtagattt
    28381 cgaagtgtaa accaggtgtc tgtcctgagt tttctcaaga agttgcgcgg ttttatataa
    28441 agcagcgcga ggattaccaa tacggtattc agaacgctta tcctggcaaa atcaataatt
    28501 ggtctgaaat gagagatccg tttttcttta tcttcataga agatttttac cggcaccggg
    28561 agaattttta ttccattcca tgacgctctt accagcactt caatctcaaa ttcaaattta
    28621 ctggtaatga aattaatatt tcttagcctg ttaaccggat acaggcggta gcctgattga
    28681 gtatcaggaa gttttattcc tgtttcaacc caaaaccaaa aattcgaaaa cttgtttcca
    28741 aaactgcttt ttgatggaat tccctgctgt tccatattgc ggctgccaat tattaaatca
    28801 cccggattct tttttgatgc ttcaagcagc agaggtaagt cttcgggaaa atgttgacca
    28861 tcagaatcca aggtaatagc ataatcgtaa cccagttcca tggctttttt aaatccggtc
    28921 cgcaaagcaa tccctttgcc ccggttggga gtgtatgata caacttcgat aaatggaaat
    28981 ttctttagaa taccggaagt atcctgagtt gatccgtcat taacaacaat aaggttccct
    29041 gaatagttta aaatctcccg aataactttt tcgagagtgg ctgtattatt gtaggtgggt
    29101 acaattacgc acgtattctg tttgcgaaat tgttcctgaa cggcttcctg agaaatcatt
    29161 aaaccaaatg tgctttcaat ttaaaaaagg ttgtttctcc cgaatacaat ttagcttcaa
    29221 aatcaacggt accttcacca ttgagaccat actcaatatt ggcctgcact tcagaatttt
    29281 gccgtggatc aatcaccgtc atgaatttca tacttgtagc gctggaaagt ctgaaacttc
    29341 tgtgtaactc ctgctccata atctccttaa ttaactgcat catgcaaact ccaggcacaa
    29401 ccggaaagtc ctcaaaatgt ccgccaaaga tggaatgggt ttcatcaaag gaaacaacgg
    29461 cttctatagt atggtttgaa tgcttaacct ctttcaatga aaaaaaatca ttgattagca
    29521 ttttcaatat ctagtttaat taagcttatt ttcatgttga atgtggtatg gttgagggtt
    29581 atttcgccag gtatccggtt atcatagttc ctgaaaaagg cttctaatac cttcttcctg
    29641 cccccttgtt ccacgcgcac taaattagtg caatcccagg aagtaacata ataatccgcc
    29701 tcattgtccg gatcaggcac tgcgtaatag taaaggtctg attttgagta acttaaaatt
    29761 ggccactcat caagtgaatg catcaggata atctcaaaat ctttcctgag tgtattaatc
    29821 accactttct tgttcaggtt atcaatcacg tagtgtgatt taaattggcc gtttttcgta
    29881 aactcaaaat caagaaattt aagtcccgat ttactggtaa aagcgatgcg catgctgctg
    29941 tcgggcatta gtttaaaaag cataagtccg ctgatgtgat tgcccataac atccacatta
    30001 gcttcatacc agttagtagt gaagcggggt ttgaattttt gcaggcagcc aggatctcct
    30061 tcagcaggaa caagagtttt gtatgcagaa ccgcaggaaa atagcaggaa gcaattaatt
    30121 aagagtaaaa atctcatcag aaagttgggc gttcagattt ctattgacaa actttattaa
    30181 tgtagtgtct ccggaacttt cattcatcaa aatttgcgta acggaaaaac tttctttatc
    30241 tgcataaatc agaatggtgt taaaaaatcc tttcagagtt ttatccaccg gtgtcatttc
    30301 catcaggtaa cgtgaagcat cttcaaaaat tcttacagaa aaatcttcat tgtcaataat
    30361 tgttccatta acgctttcaa ccataatatt attaacctgc tgaaaaagtt tgttcgatct
    30421 aacattcatt gtagtttcat tttgatcatc ttttatgatc attttatctc cattgattat
    30481 catcaggtaa taaaaaggat caaggtattc aattctgatt ttgttttctc ttttgaaccg
    30541 gaattctccc ttagaaatta ttttttctga aagtatggtc agttctttct cctgttcaaa
    30601 attgcttgaa atagatttga tctttgccga ctcatcattg aatttcttct tgaatccgga
    30661 gatatcagaa atttcagtaa agttattata ctgagcaaga aggtttaacg gtgccagcca
    30721 taaaaataat atcaaactac gcataaggtt tttcctcaat caattcatca actctaacaa
    30781 tcttcaaacc aatattccgg atatattcca gaatttccgg taagatgtct gttaaaacga
    30841 cactatggtc gtgaaacagg ataatatctc cgggcttaat tctctttttg attttattga
    30901 aaagcctttt ttcattcttt attatggtat cgagagaacg aagactccag cctactgaaa
    30961 cataattacg atttttcacg actttggcca aacgaggatt ggttactcca taagggggac
    31021 gaaaaaaccg gggttttctg ccggagactt ctttaatagc atcgtcagtc atagataact
    31081 cctcttccat tttttttgtt gattggaaat caaaaaaatc actgtggaaa aaactgtggt
    31141 tgccaagcaa atggccttct ttgtgaattc ttctgacaat gtccggattt tcatccacat
    31201 tttttcctat acaaaaaaat gcagccttgg catcatactc cttcaggata tccaggatat
    31261 atggagtccg ttgggggtga ggaccgtcat caaaagtgat cgcaacttca ctttttagaa
    31321 tgccggcttt gaaaattacg gggataaaga attgggaatc gatttttaca accccataag
    31381 taactattcc aagataaacc agagttgctc caaaataaat ccaccagggc aatgggaaat
    31441 agaaccaatc gatcagaaaa ctgatggaaa agataaatag gaaagtgata ttaattcttc
    31501 tatagatcac ttctgcaata aaataaggga atggtaaaca ccaaaataat tattataaat
    31561 taaaacatta cgaattgatg ttttaccaga cagctttctt gccagaattt caggagtttg
    31621 attgtgttcc agggcatttg tgcctaccca ggtcgcaaat gctgatgctg tgggatattc
    31681 tccggaaaga tgcttaaaga aaagtaaaga ggattcagga aaaatatttt ccactttttc
    31741 ataaatccca tcataccgtg tatctccgga tctgccggtt aagagaacgt ctatctcagt
    31801 tggattaaga ttattttcgt tgagaaactt caacagactt tctatgacgt gagaagcatt
    31861 tgcgggtttg taaatggttt ttattgactt aatttcagcc aacgcgtttt gcttgtttcc
    31921 tgaaagaaca aaataagtgc atccttctcc atccatggtg ccttctgcag gcagattcag
    31981 aagactcaaa ttggaaacgt tttcttttcg gtacaaatca aatctgttca ggatcttatg
    32041 gctaacctca atgatttcat caactccgcc taccagcaaa ttccaaccag ggttgtcagc
    32101 aagcaggaga gacgcatcca ccagacaatt ttcaaatgaa aaccctcctt gagtatacgt
    32161 cgaattgtat ccattacaat gctgtagcaa tgcaatctgt cctgacatcg tattatgcgt
    32221 agattgaata aatggtgttg ggttaagcgc tacctcctta ctgtcaatca ttttacgcaa
    32281 aaaattctca gtatccgcca tacagccata agaagtaccg gttattattc catcaggtat
    32341 ttctactccg gcttcttcca gtgctttgtt tgaagccgct acactaattc gtattatttt
    32401 gctcatcctc cggattgcgc gcggctctat gatcccttca taagagggtt caacagcatt
    32461 tagctggttt ccaataaatt cctgaatctc tgataaaaag gaattgttct caaaagtctt
    32521 ttgtggtgaa attgctgcca tgccttttat aaacacgctc atatcagcag gatgaaaaag
    32581 ttaatgacga acaattacct ccaaaaccga aactgttgct catcacgtga ttcacctcaa
    32641 ctcctttttg aaatgacttt aggggtgcaa attttatttc agacatttgg tgattaaagc
    32701 gaagatttgg ataaacaact ttactttgaa tagatgcaat ggaatatacc gcttcaattc
    32761 caccgcttgc acccagggta tgtccggtaa aggattttgt tgaactggct ttcggtacat
    32821 tttctccaaa caaacgttca acggcaattc cttcggagga atcattgttc tgagttccgg
    32881 ttccatgcaa attgatataa tcaatatcgg tgggcataag atttgccatc tccaaagcag
    32941 acttcattgc agaaaatgaa ccgcggcctt caggagaaga tgccgtttga tgaaaggcat
    33001 cattggtgtt ggcgtatccg gaaacgatag caaggggtct aaccttttct tgttcaagaa
    33061 ctttttctga aactagtact atatatcctg caccctcgcc caggttcagt ccttttctgg
    33121 tttcatcaaa tggacggcag ggttctgaat caagaatcat caaactattg aagccgttca
    33181 gagtgaatct tgttaaagca tccgtgccgc cggctatcac aatgtcaagt tgatcgttcg
    33241 cgataagttt cgatcccagc ataatagaat ttgctgaaga tgaacaagcg gtatttatgg
    33301 ttgagatgaa atcctgtatc tgaaaatggc tggcaattag ttcggttatt ttgccgcact
    33361 cgtggttctt tatttgatgc aatggcgcag aatcttcatt acgcataaat gtttcataat
    33421 actgctcact cttgtccatc ccaccaactg tggttccgga gatgattccg cagcgccatt
    33481 ttcttaattc cggaattcct gaactctgaa ttgcttcttt aaccgcatga attccaagaa
    33541 gtgcggtccg ggttaatgtg gtggggagct ttaaagaaga agcaagtttt tcattggtga
    33601 ctttaacttc tcctaccggc agtttgtttt tgtgaatact ctcgaacaag gtaattggcc
    33661 ctattcccgc ctgatgattt tttaaagctt ccagattttc ggcaagatcc tttccaattg
    33721 ccgaaatcac tcctaatccg gcggcataag ctttactttt caagcagact gaggcttttt
    33781 attattctca atatattctg ccatagattt aactgagcga aagatctggg gaccctgaga
    33841 gggatcctcc acctttacac cgtagtgttg ctgcagcagt acaatcaatt ccaaggcatc
    33901 aattgaatca agtccgacac cttcaacaaa aagcggttca tcatccttaa tatcttcagg
    33961 tttcagatgc cttaagttta aagcttcgat tatctgaacc ttcagtgttt gcattaactg
    34021 ttccattagt agttgttata ttttttcaac aaatcttcca tagaatggtt gaaagaatag
    34081 tctgattttt ttttctccac caaatataaa aaaacatcgt ggttctcacc taaaacttcg
    34141 acccaacctg acaaacatgc ctcagtttga ttgttttgca ataacaaatc tacatatttc
    34201 accatatatt ctgggttaaa ggcctcgtga atgaaaaacg cgttttcgcc tttgaattta
    34261 tttcgaatac atatttcacc gattactatg ttgggaaggg tataaacaaa aagggcaggg
    34321 cttggaattt tctcagttga tttctgatat cttaaatcgg tatcgaggct tgagctgtga
    34381 ttagataaca caacggcaat atcttccggc ttatattttt ggttagggtt ttcattttga
    34441 aggagcagtt cagatgcaat cacacccgct ttgcttagat tatccatttt ataatattta
    34501 gtatatattt cgcccaacgg ataaatcttc aaaaggaaat catcaaagga agattgatga
    34561 tcctcgtaaa taagaatatc atttacagtg actttcctgt tgttaatcct gcaaaatcca
    34621 ttaacaaagt atttcatcat tcattaccgg gagagctctg ccatttttat ttggaagtca
    34681 atgaacttcg atacgtttcg taatgaaatg gaatgattct gaacaaaagg attattcata
    34741 tcccaaacat atcctgctaa aacactgcag atttttttct ttatttcttc atttggttga
    34801 ttatgaaaaa atatcttttc tagagttcca tcgtaccagc cattcacata ggatcggaat
    34861 gtgtccacac cctgttgcat cacatcggta tattcagttt cccaatccac ttctgcacct
    34921 tgcaatttct tcaccaccag gttagccgcg agatgacctg atactgccgc aaacataaca
    34981 ccggaggaaa aaacaggatc caggaattcg gtggcatttc ctgccagaac aaatccgttt
    35041 ccatgaaact tctttgtctt tattgaccag gcttccagtt ttcggggctc gaacacaagc
    35101 tcctgatcct ggaatctttc acgaagatac ttgtttcctt ccaccagagt tttaaactgc
    35161 tgattgatat ctccttcaag tttttcaaag taatccatgg agccaacaaa tccgagggag
    35221 gtagttccat tggaaaatgg gattacccaa acccaggttc ccggtgcgta aacgataatt
    35281 aaaattctgc tgggttcttc aaagtgcaat ctcctgttgt cttgtatatg aacaaagaca
    35341 gctttccttg gatccagtcc tgaaggttta tccaggtcaa acattcttgg aatcactctg
    35401 ccatagccac tggcatcaat tatatacctg gcggtaataa ccgatgagtt tccgtcttta
    35461 tcaacaactg tggttttgct gtagccatct tctgcgaatt caattttttc tactccggtt
    35521 tgaaaatcaa tttcaattcc ccgctgctgc agggaatctg tcaaagcttt atcaaaatct
    35581 gctctggtaa cctgccacgt ccagctatat cccggagtaa aaccctgtga aaaattgaaa
    35641 tcacaaatag cgtcatcttt catgaatttg gccccgaatt tttcctgaaa cccgcatgct
    35701 ttgatggcat caagcaaatc agcctcctcg aaagcatcca tgcaacgagg cagcagactt
    35761 tcaccaatta caaaccttgg aaattgttgc ttctccacaa cttttacctt aagacctttt
    35821 ttgtgaagca ttgcagcggc tacacttccg gcagggcccg ccccaattac caatacatct
    35881 gtctttaatg aattcatctt cacgccaatt tacattctat taacgattga cacacgccta
    35941 aatcccgagt ttctttaata atttgaaaac cggtttcgtt caaataattg aacaagtctt
    36001 ttgaatagaa aaatttgctg tttccatttg caatcgtggt aaagtagagc gatgtctgca
    36061 taaggctaaa ggcgccacct tcaaattttt gttgatccca catgggttcc acaatgaaaa
    36121 ccctgccatc aggatttaga gcatccttgc aacgtgtcaa gatagaaatc acctgttctt
    36181 cagaaaagca atccaggaac tgactcatcc aaattgcatc aaatcccccg gggaatggtt
    36241 tattttccaa aagattagtt tgataaaact tcactctgtc tttttcccga tgggattcca
    36301 gatttttcct ggcctcgttt aattgaattt gcaaatccat caaagttatt tccacgtcct
    36361 tgtcgaattc aatgcattgt attgcccact tacccgtatt accaccgatg tcaagcaatc
    36421 gaatgggttt attggcaaat acgaggggaa gaatttccgg aaaggcggta tcagaataaa
    36481 agtgatcaaa atcaaaccag ctcttaagtg cttttcccgg gaggatagaa aggccttcat
    36541 aaattgtttt ccagggtccc agaaatctta atccctccgg ctttgcattt tccagacttt
    36601 tctccaaatc gaaaattcca ttataacaga cgtgctgggt gaagtccatg ttaatcctgg
    36661 tcatttcatc cttaagaata aaccagccaa ctttagtgac gaaatatttc tcatctttct
    36721 ttattagaat tccagaagca aggcatgact ccatcaacat ctgcacctgg tatttgttgt
    36781 atttggattt ttgtgaaact tcttccacag aaagtccggt atcactatta tcctccagga
    36841 tgtccagaat tccactgtcc ctgattaccc ttgcagcctg gaaaacaaat ggagccagtg
    36901 ctatccattg ggcatcacgc atcgcagtta ttccattctt gccatcttta ctaaagaatt
    36961 ccatcaatta ttctgttttt tcgaagatta aggttgcgtt acatccacca aagcctgatg
    37021 cagtttttaa gaatcgcttt agcggtttca tttctttttt cctaatcaca ttaataggtt
    37081 tggtgacccc gggtgtttcg tatcccatgg aagggattaa ttcgttattt agcaaagaca
    37141 taacactgat tgcagattca agcacccctg ctgctcccaa agtgtgacca taatatcctt
    37201 tcaaactatt gagcgggaca ttgctgaagt cggtaagact gaaggcattt gcctccattt
    37261 catcattaaa caacgtaccg gttccgtgag cattgataaa atcaatttcg ctaaccgtaa
    37321 cttttgaagc ttgcatagct tttcttatgg caacacctaa ctcttcgccg gttctgcttg
    37381 gtccggaaat atggttagca tcgttggagg tcgctccgga agttatgcaa accggaatct
    37441 ctcctttttc tttagtaatg atgatggtag cggcagcttc ccccatattg atgccttttc
    37501 gatttgcatc aaatggttta cagggttcag agcttatagc ttgcaaactg ttgaagcctg
    37561 aagtaataaa atctgaaatc aaatcacatc cgcaaaccac tgcatttctg tataagccgg
    37621 cctcaattaa tcgtttagcg gtgagaatcg ccaatgatcc actgatgcag gcattcgaga
    37681 tcacaaccgg ttgatttgga ttattaaaat atgatgaaac tttccggctg ctttcaaaaa
    37741 gatttacctt tttaagatca tttggtttgc taagggaatc aatttctcct tttgtgctgc
    37801 tgataatgaa gattgtatct ttatcagaag gggcagctga agtttgcgaa agggccgctt
    37861 gaatggattg aatcattaat gattcaaaga ctccgaaatt gtcaaccaaa aaattttctc
    37921 tttgaagtat ttttccgcca taaacggtat tgattccaat ctgaacaggg gcaactccgc
    37981 tttttccatc aataacagat gaatagtttt cataggtagt tttacccaat ggagaaatga
    38041 tgttgtgcgc gtctacccag atcctttcca ttcaataaat caaatcagat tcatcttttt
    38101 tttccattcc tcaaaaaatg cgggaactgt tagatgaagg tcgaaagttt tatggttaac
    38161 aaaaacctgg gttgatgaac cctttgcaac aatatttcct ttttttgaat gccggattag
    38221 atattcaaaa taaatcttgg cgctctgagt attatggtaa gtgacttcaa catccataat
    38281 atctccatac cgaagcgggc ttttgaaatc gcaattaacc gagacgacag gaaccacaag
    38341 tccattattg tagaagtcga gataggagac tccgtatttc cggccaaact cttcacggcc
    38401 atcctccata aaccggatat aatgaccgtg ccacacaata ccaagcgggt cagcttcatt
    38461 gaagcgcact ctgacctgag tagtgtgctt taacaaagag accttgtccg ataaagtttc
    38521 ggattcttcc cgggagactt gcttcagaat gttttctccc aagtcttata tcttttctta
    38581 tcaatctgaa ttattgcctc gaggattgat ctggcccaat ggttacgaag tccaaatcca
    38641 gcggaaggtc cggaaagtct ttcttcataa atgatttcct tattgttcag gttaacaaaa
    38701 gtgagtgcca ttgtgccttc cgctttattc ttgttgaaag attcaattac aaacatcatc
    38761 ccaagcccgg tcaaatcttt aaagtcatag ctgctcatga tgttttgaat ttgttcggaa
    38821 gttagcggtt tgccaggatc tgtgaatgta atcttttctt taaagtttac attgtcattg
    38881 tggtcaatac atatacctac ctggtaatcg atgttgttgt tacgtaatgc tcttcggagg
    38941 ttgtacttat cagcttccga aaccataagt tgattccact gagggttcag ccttttcaat
    39001 tcgtcttcac tcttccactc ctcataacct cccacaaaaa gtgtttcggt gagatcgatg
    39061 ccaagaaaag ttatcttgct tgagtttaag atatctctct tggtgagttt taattcaggc
    39121 ttactactgg tcgtagcgcc gccccgtgaa gatggaggag gcttatctgc tgaagattgt
    39181 gccatggtag cggtataggt tagcactacc aacattgcaa ccattgctat actgctgtac
    39241 tttttcatct tagaattagg ttttaatttg actatttttt gataaaaatt ctcatctcac
    39301 acttggctac ttcttcatta ccaattttaa caacggcatc aaccattgtt atatccaaaa
    39361 ctcgactacg gatatttatg gttgtagtaa tgtgagtgtt tattaatggc aagttagtga
    39421 tttctaagtt tttaaccata gcaatatatc caatagggac aggaatatta ttcagagaaa
    39481 aatagtagcc tgcctggaca gcagcagttt gggccatatt ttcaatgatt ccgggttctg
    39541 taagttgatt ttcaacaaca aaaatattgt ctggctgaat atcaaattcc gttgtggcgc
    39601 aagactcatt ggcgtcaata agtcttgaaa tcataaccat tggctctctt tgcggaatta
    39661 agctgagaat atcgtccgtt ttgggcaaca tgggattatt tctcaaactt ataataatta
    39721 aaccactgaa aaggatactt tttcaccatt ttctcaaaaa gttcagcaaa ttgtgttgcc
    39781 atatatttaa tataatctga ttttgccaat gaagagtcat aatcaattat tggtgagcca
    39841 taaaaatggt aatgccgcag ggtttcttta aaggaaaaaa caaaggaaac aggaacctta
    39901 aaagtagctg ccagagcaaa aggaccaacc gggaaaagcg ctttttgatt gaagaaattc
    39961 atttccaggg tttttgcttc atccacaaac ctgtccgcat ggaggcatat caattcattg
    40021 cgaagcaggg cttctccaat ttcataaaca tgtgacaaat cttctttcat tacaatcagg
    40081 ttgtaatttg caaagaggat ctggtttgca tactctttaa tccaccgatg ctctgcatca
    40141 aataccacaa tattaatttt agccttcaac ctcttgagca ggtctccggc aatttcccaa
    40201 ttgccgacat gcccgctaag caaaattcca cctcttccca aggagacaat ttcatgcagg
    40261 ttttgttcgc cgtcgaaagt gaaagtcagg ttgttcttaa tatcccccat aacgacgaat
    40321 ttgtcaataa gtacctggcc aaaaatgtaa tagttccggt agatagccag aatcgatttc
    40381 gctactgaga atttaaatcg atttcgaaaa tgacgataga tatgcttaga ggactcccaa
    40441 ctaaacagga aataataaaa ggcgacaaac aataagacaa aataggccgg aaacaggccg
    40501 aacttgcggc aaatgaaaac gaaaatttta taccctgatt tattgccttt ggatttaccc
    40561 tgccattgag gcataattat gcatccactt tggaatcaat gcgggaaaag atgtaatcgt
    40621 ataagtcctg aaatgttctg attgaagtaa aatcttcagg tttaactttg aatccaaagt
    40681 tattttcaat agccactacc aggtctatat aatcaagact atccagatca agagtttctg
    40741 cgaggcttcc atccggggtt attgaagaaa cctcaatttc aaattcttct gccagaaaat
    40801 catttatctt ttcaacaatg ctattcttat ccatctaatt aagaattttt ttcacaatta
    40861 aacttgagtt agtaccgcca aacccaaaag agttcgacaa aaatacatta atattttgat
    40921 taattcgctt gtccgctatt ttgagtttag ctgagaattc atcaggagtt tccagattga
    40981 tgttaggagc gatgaaatca ttttccatca ttatcatgct ataaactatt tcacttgctc
    41041 cggccatcca acattcgtgt ccggtcatag attttgttga acttacccag ggtttgtgct
    41101 gcccgaaaac attatcaatg gctctcgctt catttgcgtc gccaacgggt gtggaagttg
    41161 catgtgcatt tatataatca atctccgacg ggttgatgtt tgcatctttc aaagcccttt
    41221 gcaaagacct gctggggcct tccacattcg gattggatat atgatctcca ttggaagaaa
    41281 agccatatcc gataatttca cctaaaatat tagctcctct tctttttgcg gaatcaagac
    41341 tttctatgat tacagtggca gcgcccccac ttggaacaag gccatcccga tctttatcaa
    41401 aaggccttga ggctgccgcc ggattgtctt cccttattga aaatactcct aatccatcga
    41461 agttacccat ggaataaata tttgtttcct gagcaccgcc gcaaattatc atgtcctgca
    41521 aaccattttt gatatacaaa aatccaatgc caatggcgtg tgaactgctg gcacatgccg
    41581 cactgacggt gaaattaatc cccttcagtt tgaatatggt ggcaaggttc atgttcacag
    41641 ttgaattaag agattgaaaa acagagccgg aaccaaccat catggtgtcc tttttggttc
    41701 ttattatgtc tattgcttca ataacagcct tggctgaact atcattaccg tacagaatgc
    41761 cgacttcatt tttgttgaaa tactcctgtt ctaaacccgc ctgctgcata gcttccagtg
    41821 tggcaacgta ggcgtattct ccttgttccg gcaacatgat tctggctctc cgatctaata
    41881 tccctttcaa taccggtttc tcaaccttgc ccgttagtcc agaccggtaa ccggtttcct
    41941 ttcttccctg gtccaataca attcctgatt taccattaca caaagaatct cttacctcgg
    42001 atctgtttag accaatggtg gaatagattc ccaaaccggt gataacaact cgatgcatac
    42061 attaaagatc cggtgtttgt agatattaag ataaaaaggg gtttaaatcg aagttctaaa
    42121 ccttcaatat tccggcaaaa ctaacttccc aataattgta aatcaagtat aaaggcctcc
    42181 attcactgaa attactgtgc ctgtaatgta agcggccttt ttggaagcca aaaataccac
    42241 taaatcagcc acttcgtccg gttctccaaa ccttttcatt ggaatcaaac gctttaattc
    42301 ttcttcattg agatcttttg tcatgtcagt ttttataaac cccggggcaa ccgcattaac
    42361 ggttatacct cgtcggccgc attcctgggc tagagcttta gtagctccta taactcctgc
    42421 tttggcagct gagtagttta cctgtcccgc catgccactg atacccgata agctgacaat
    42481 atttattatc cttccgctct tgcgcgagag catgccttta ataatgtgat gagttatgta
    42541 atagaatccg ttgagactgc ttcccaggac atcgttccat tgttcttctt tcatccacat
    42601 taagagggta tcctgcctga tgccggcgtt attaaccagc acttcaattt ctttgccggg
    42661 atttgcttcg atccaaccgc ctagtttttc gtctacttct tctttttttg aaacatcgaa
    42721 ttgaagtaat tctccatccg ctcctttttc ctttaccagg ttcagcgtct tctttgcttc
    42781 aatttcattg cttttgtaat taatcaggat cagaaatcct tcttcagcaa gtttcacgca
    42841 aatggctttg ccaattcctc ttgaaccacc ggtaactaat gcatatttca tgattaaaat
    42901 tttagagatt ttctattcat taaatattct ttaacggctt tcaattcttt ataccttgct
    42961 tcatcttcaa caaaagtcgg gataatggca cggatatcat cataaatagc ttttgtaaaa
    43021 gaagaaattg atttatgaca ttgaagtaag tcgatggcct gcacaagggc aacgatctga
    43081 acagaaagaa tttcaaagct gttgtcaatt acttttcttg ttaatagtgc cgcattgcac
    43141 cccatactaa caatatcctg attgtcattg tttgtagaaa tgctatgtaa atacatgggg
    43201 taggaaagag tctgattctc agccgcgttt gaagtggccg taaattgcat tccctggatc
    43261 ccaaaattta atcctaaggc gccaaggtta acaaacggag taagcttttg atttaaatta
    43321 ttattcagca gatagttcaa ttgccgttcg gccagcattg aaagtttggc aacagctatt
    43381 ttgattttat ccatttcaaa ggaaatgtaa tcaccatgaa aatttccacc gtgaaataca
    43441 ttgcctgact tgtgatcaac gatcggattg tcattcacag aattaatttc attctccaaa
    43501 actcgctgag cctgtatgag agtatcgtac acgggaccta atatttgcgg tacgcaccgt
    43561 aatgaataat actcctggac tttctctttg aataccgatt cattgatctt ccggttgtat
    43621 aaatggtcag ggcgtttccg gattagtttg ctatcagaaa gcaggttgcg cataatttca
    43681 gcaacttttg cttgcccttc atgattcttt acctggttga gttcctttga aaaatgatca
    43741 tcgtaagatt gcattaattc attggtaata acagacatca taattgccct gctcagcaat
    43801 cgttgggcat aaatcaaatt caccactcca acgccagtca tagcggaagt accattaagc
    43861 aaggcaagtc cttcgcggat atgaacatcg atcggctgca acccttcaat ttcaaaaacg
    43921 tctttagtgg ttataatatt acccttgtaa agcatttcgc cttcaccgat caggttcaat
    43981 gcaaggtgcg ccaattgaac caggtcgccg ctggcaccca caccgccatg ttcgaaaata
    44041 caagcgccaa tccttttatt gatcaaatca gtcaggagct gtaccaattg tggattgatt
    44101 ccggaataac cgcgcatcag actaattaat ctgcacaaca tggtggacag agcaagttct
    44161 tcggtaaatg gtgcaccgga tccggaacta tggcttctaa tcaggttgta ctgaagcttt
    44221 tgtaagtgct catcatcaac ccggtattgg gccattgggc caaagcctgt attaatgcca
    44281 tatattactt tgtccttgct gtagctttga aggaaatcaa aattagcctg aacattctgc
    44341 accgcctttt gatcaagttc cacgagttcc tgatcaaaaa ggatgttctg aaaatctgtt
    44401 attgaaatat tccggctgcc gaccttaacc attgatgatt gtgctttgga atgcaaaaat
    44461 accaatgcgg atataaaacc aaagggaaac tggaaaatcc ttggtattta atctgcacca
    44521 aaggataacc tgaaaaacct ttgtaccatg aaattattca gtattcacta ttatttcaat
    44581 taatatttac ccaaggtgaa gaggttaaat acattaaact cccttaaatt ttttgactag
    44641 atcatcatta tttctatgtt gaatttggtg taaattcttg gcataacttt aattgcagct
    44701 gtagagtggt gcaaagaatt ttgccttctg ctgagctttc ctgattaatg catactcatg
    44761 aagaagttct tcgcctgaca tccatttacc aatactcaaa gaagttaatc tttttaggat
    44821 aattccctat agggtgaaaa gtcacattat ttctccactt cttcgccaaa gtgtgaccat
    44881 tcaatccatg aaccatcaaa gattttaaca ttctcatacc ccagtaaatc ttttaaaata
    44941 aatccggtat gagcggcgcg aactcctgaa cggcaataag ttatgatttg tttgtctgga
    45001 gtgattccca acttttcaaa ttgttctttc aatgcttcac tggatttaaa tacattttcc
    45061 ttttccagat tattcgtcca ttctaaatgt at
//
LOCUS       DASWHX010000072         6583 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_3014951, whole genome shotgun sequence.
ACCESSION   DASWHX010000072 DASWHX010000000
VERSION     DASWHX010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 6583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6583
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3014951"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            98..1198
                     /locus_tag="VGA21_05090"
     CDS             98..1198
                     /locus_tag="VGA21_05090"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX9649913.1"
                     /translation="MGVSAILFLSFTLVSYVFLILLFLAAGQESMPARNLIEYPVSVI
                     VAARNAKAGLEKLLMTLKDQHYSEFEVVVVDDRSTDETSSMLEELKRDYSWLKVVRIE
                     KTPENADPKKFALTMGIKKAKHDILLFTDSDCFPGSSGWISGMANSFDSETMIVIGFS
                     NYETRHGLLNYFIRFETLWTGIQYMASAVMGNPYMGVGRNLAYRRSFFAKKKGFRSFA
                     NVTGGDDDLFVNKNATSSNTKVALGKEVLTLSQPKIRVKEFLDQKLRHLSVGKRYQLK
                     DKLLLGFFNISFLGTLVITLVLIAKSSVLYWLASFLLVRWLLMMVIIGRWSKILGSPI
                     NVIGVPILDILYLFYYIFTGTRALFIKKVKWT"
     gene            1189..1797
                     /locus_tag="VGA21_05095"
     CDS             1189..1797
                     /locus_tag="VGA21_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010611316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX9649914.1"
                     /translation="MDLKKQFSAKALEDFKLIDKAILDRDQQAFAELLHRYRKPVYHM
                     ILRMVRNIDDAEDLTIEAFAKAFKNLHRFKKDFTFSTWLFRIATNNSIDFIRKKKLET
                     TSLDTSYTDDNGEAVSMDVEDKGLNPQEEAIKSQKVELMQMFVTKLPAKYQRLVRLRY
                     FDELSYEEIAKELDAPLGTVKAQLHRARELMYDLVKGSEKHM"
     gene            1801..2433
                     /gene="rsmG"
                     /locus_tag="VGA21_05100"
     CDS             1801..2433
                     /gene="rsmG"
                     /locus_tag="VGA21_05100"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002705937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEX9649915.1"
                     /translation="MDSPEILQQYFPLTEIQKKQFQQLQDLYTDWNQRVNLISRKDID
                     NLYERHVLHSLAIAKVLEFNSKTHILDVGTGGGFPGIPLAIYFPECQFHLIDSIGKKI
                     RAVEEIIYSLGLENCYASHARAEHVKDKPQFVVSRAVVEIPKLIKWVKNKVSSKSTHH
                     LPNGLLCLKGEDVGMELKTIKQAFHIFPVKNYFREDFFETKVVVHVEMDF"
     gene            2852..6349
                     /gene="dnaE"
                     /locus_tag="VGA21_05105"
     CDS             2852..6349
                     /gene="dnaE"
                     /locus_tag="VGA21_05105"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011583504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEX9649916.1"
                     /translation="MPQFSHLHCHSQYSLLDGAAGISDMFKKAQKDGMRAIALTDHGN
                     MFGAFKFVSEASKYDVKPIVGCEFYLVENRHKQQFSKEDKDNRYHQLLLAKDAAGYKN
                     LSKLCSLGYMEGMYSKWPRIDKELILKYHEGLIATTCCLAAEIPQAILNKGEEAAEEL
                     FKWWLNLFGSDYYVEIQRHKLEEQGVVNEVLQRLAAKYNVKMIATNDSHYIDQEDANA
                     HDILLCINTGEFQSKPIWNGAGGGGKDYRFGFPNNEFFFKTQKEMEAIFGDLPEALDT
                     TNEIVDKISILDLRRDVMLPNFPLPEGFKNSDDYLRHITMEGARKKYSQITPELEERI
                     NNELGIIRHMGFSGYFLITADLINAGRGMGVLIGPGRGSAAGSVVAYSIGITNIDPIK
                     YNLLFERFLNPERISMPDIDTDFDDDGRQKVIDYVVQKYGFNRVAHIITYGTMAAKMA
                     IRDVARVMEMPLSEANALAKLVPERPGITLELAYKEIPDLAEIRKGNDSRAEVLRLAE
                     KLEGSVRNSGIHAAGVIIAPDDLPDLIPVSTSKESNLLVTQFDGKVIEDAGMLKMDFL
                     GLKTLSILKDAIKLIKKNKGVEINIEEILLDDEKTFKLYQTGDTIGTFQFESAGMRKY
                     LQVLKPTNLEDLIAMNALYRPGPMDFIPNYINRKHGNEKVEYPHELLEPILKNTFGIM
                     VYQEQIMQSAQILAGYSLGQADLLRRAMGKKKKEEMDKQKEIFVAGAKEKHGIEKEKA
                     EEIFSIMERFAQYGFNRSHSAAYSVVAYQTTYLKAHFPAEYMSAVLTHNQANIDKITF
                     FLDECRHQNLVILGPDINESNIHFDVNREGQIRFGMGAIKGSGEAAVEAIISERNENG
                     SFADVYDFASRVNLRSVNKKTFEALALAGAFDSFGQIHRRQYVHAENGENSLIEMAIR
                     YGNAQQQEKNASQQSLFGAGTGTAISKPKAPVCEPFGEIEKLKIEKEMVGFYISGHPL
                     DQFRIEMKEFVTCPVRDIEKYKNQKITLAGVVTKFTERFTKAGKPFGLFTIEDYEDSI
                     DLAIFGEDYLKNRHMMMLDNFVYLTGKVEERYNQPGVWEFRTWNIQLLGDIRSNLSKE
                     IILRVLLQDIDENLVRNLAELAQASPGNCTLKLNVFDPDEKISLDFLSKQYRVSPSNQ
                     FFDKLGRFSEVKYEIIKSN"
     gene            6456..>6583
                     /locus_tag="VGA21_05110"
     CDS             6456..>6583
                     /locus_tag="VGA21_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014772251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEX9649917.1"
                     /translation="MSKPIELTDANFDEIVTKSEKPVLVDFWAEWCGPCKMIHPVV"
BASE COUNT         2044 a         1200 c         1479 g         1860 t
ORIGIN      
        1 caaagcaata aaaaagtgga tgattctacc cggacaatca tccctttgaa tggtaatttt
       61 gtaacattgc tttctttctg ccatactcag atgtatcatg ggagtttcag ccatcctttt
      121 tctttccttt actctggttt cctatgtatt cctgatccta ttattccttg cggcaggtca
      181 ggaaagcatg ccagctagaa acctgattga atacccggtg tcggttattg ttgctgccag
      241 aaatgcaaaa gctggattgg aaaaactttt aatgactttg aaagatcaac attattctga
      301 atttgaggtt gtggtggtag acgatcgctc gacagatgaa acttcatcta tgctggaaga
      361 attaaaacgc gactattcat ggttgaaagt ggtgcggata gagaaaactc ccgaaaatgc
      421 agatcctaaa aaattcgcct taactatggg catcaaaaaa gcgaaacatg atattttgct
      481 ttttacagat tccgattgtt ttccaggatc atccggatgg atctcaggta tggcaaattc
      541 ctttgattct gaaaccatga ttgtgattgg attttcaaat tatgaaacca ggcacggatt
      601 actgaattat ttcattcgct ttgaaacgct ttggacaggc attcaataca tggcttccgc
      661 agtgatgggg aatccttata tgggggttgg aaggaatctc gcttaccgga gatctttctt
      721 tgcaaagaaa aaaggctttc gaagttttgc aaatgttacc ggtggagatg acgatctatt
      781 tgttaacaag aatgcaactt cctcaaatac taaagtcgca ttgggtaagg aagtgttaac
      841 tcttagtcag ccaaaaatca gagttaagga atttcttgat cagaaattac gtcatttatc
      901 agtaggcaag aggtatcaat tgaaagataa actattgctt ggctttttca acattagctt
      961 tcttggaacc ttggtaataa cgctggtatt aattgctaaa tcatcagtat tatattggtt
     1021 agcaagcttt ttgcttgtac gatggttgct gatgatggtc attatcggac gatggagcaa
     1081 gattctcggc agtccgataa atgtaatcgg agttcctatt ttagacattc tttacctgtt
     1141 ttactatatt tttacgggta ccagggcatt gttcatcaaa aaggttaaat ggacttaaaa
     1201 aaacagtttt ccgcgaaagc tcttgaggat tttaaattaa ttgataaggc gattctcgac
     1261 agagatcagc aggcatttgc tgaactgctg catcggtata gaaagccggt gtaccacatg
     1321 atcttacgga tggtgcgcaa tattgatgat gccgaagacc ttaccattga agcatttgcc
     1381 aaagctttta aaaatctcca ccggtttaaa aaagatttta cgttcagcac ctggttgttc
     1441 cgaatcgcaa ccaacaactc catagatttt atcaggaaga agaaattaga aaccacgagc
     1501 cttgatactt cctatacaga tgataatgga gaagcagtat cgatggatgt ggaagataag
     1561 ggattgaatc cacaggagga agctattaaa agtcaaaagg tagaacttat gcagatgttc
     1621 gtaaccaagc ttcctgccaa gtaccagcga ctggtacggc tgcggtattt tgatgaactt
     1681 tcatatgaag aaattgccaa agaactggat gctccattgg gaacggtaaa ggctcagctc
     1741 catcgcgccc gcgaacttat gtatgacctg gtaaaaggaa gtgaaaaaca catgtaaatc
     1801 gtggattcac cagagattct gcaacaatat tttccattaa ctgaaattca aaaaaaacaa
     1861 tttcaacagc tgcaggattt atacacagat tggaaccagc gtgtaaatct tatctccaga
     1921 aaagatattg ataatctgta tgagcgccat gtattacatt ccctggctat tgccaaggta
     1981 cttgaattta attctaaaac acatattctt gatgttggca caggtggagg tttccccgga
     2041 attccattgg caatatattt ccccgaatgt cagtttcatt taattgatag cattggaaaa
     2101 aagatcaggg ctgtggaaga gattatttat tccctgggtc ttgaaaattg ttatgcttcc
     2161 cacgcgagag ccgagcatgt taaagacaaa ccccaatttg tagtttcacg ggccgtagta
     2221 gaaataccaa aacttatcaa atgggttaaa aacaaagttt cctcaaaatc aacacatcat
     2281 ttgcctaatg ggttgttgtg tttaaaggga gaggatgtcg gaatggaatt aaaaaccatt
     2341 aagcaagcat ttcacatatt tcctgttaag aattatttca gggaagattt ctttgagact
     2401 aaggtggttg tgcatgtgga aatggatttt tgagtgaaga ttgtgaaaag tcggggttgc
     2461 cacaccgggg aaacctaaaa agaagttagg tgtggcacac ttctaaattt atttctacct
     2521 aaaatcaaat tttatgcatt atctgaaaag tcaggtgtgc cacaccgggg aaacattatc
     2581 tgaaaagtca ggtgtgccgc acaagggaaa tagcagggga aagagggcgg ggacaattca
     2641 agcggaactt taagtgaaaa aattaataag atgatcttca agtagttcag caaataggaa
     2701 tttcgcgaga tataaaaatg tcctacattc aacttcgaat taaggaaaaa atcttccctc
     2761 attatctaga tttgtgggtc cagcaacact taaagtaagt tctgtttttt ccattatgtc
     2821 agcgatttcg cccaaatatt aatttattaa catgccgcaa ttcagtcatt tacattgtca
     2881 tagccagtat tcattactgg atggagcagc cggaatttct gatatgttta agaaagctca
     2941 gaaagatggg atgcgcgcaa tcgcgcttac tgatcatgga aatatgtttg gtgcctttaa
     3001 gttcgtttca gaggcatcaa agtatgatgt taaaccaatt gtcggttgtg aattttacct
     3061 ggttgagaac cggcataaac agcaattcag caaagaagac aaggacaacc ggtatcatca
     3121 actattgctt gccaaagatg ccgccggata taaaaactta agcaagttat gttcgctggg
     3181 ttatatggaa ggcatgtata gcaaatggcc aaggattgat aaggagttaa tccttaaata
     3241 ccatgaagga cttattgcta ctacctgttg tcttgcagcg gaaattcccc aagccatact
     3301 taataaaggt gaagaagctg ccgaagaact tttcaaatgg tggctcaatt tatttggcag
     3361 tgattactac gtagaaatcc agcggcataa actggaggag cagggagttg taaatgaagt
     3421 gctccagcga cttgccgcaa aatacaatgt gaagatgatt gccaccaatg attcccacta
     3481 tatcgaccag gaggatgcca acgcgcatga tattctactg tgtattaata ccggcgaatt
     3541 tcagagtaaa cctatctgga atggtgcagg tggcggtgga aaagattatc gctttggttt
     3601 tcccaacaat gaattctttt ttaagactca aaaggaaatg gaagctattt ttggtgatct
     3661 tccggaggcc ctggatacca ctaatgagat tgtggacaaa atttccattc tagatctccg
     3721 aagagatgtt atgcttccta attttcctct ccctgaagga tttaagaatt cagatgatta
     3781 tctccgtcac attacgatgg aaggcgcccg gaaaaagtat tcacagatca ctcctgagct
     3841 ggaagaaaga attaacaatg aacttggaat catccgacac atgggattct ccggctattt
     3901 cttaataacc gctgatctga taaacgcggg ccgcggaatg ggtgtactga ttggtccggg
     3961 ccggggatct gctgcaggat ctgttgttgc gtacagcatc ggaataacca atatagatcc
     4021 aataaaatac aatctgcttt ttgagcgatt tctgaatccg gaaagaattt caatgcctga
     4081 tattgatact gattttgatg atgacgggcg tcagaaagta atagattatg tggtgcagaa
     4141 atacggattc aaccgggttg cgcacatcat tacctatggc actatggcag caaaaatggc
     4201 aattcgggat gtcgcccgag taatggaaat gcccctttct gaagcgaatg cattggcaaa
     4261 gcttgttcct gagcggccag ggattacgct ggaattggct tacaaggaaa ttccagactt
     4321 agctgaaatc cggaaaggga atgattccag ggccgaagtt ttgcgattag cggaaaaatt
     4381 ggaaggatcg gtgcgaaact caggtatcca tgcagcaggt gttatcattg cacccgacga
     4441 tttacctgat ttaattccgg tgagtacttc aaaggagtct aatttgttgg ttactcaatt
     4501 tgatggaaaa gtgattgaag acgccggcat gcttaaaatg gattttttgg ggttgaaaac
     4561 tctttccatt ctaaaagacg ctatcaaact cattaagaag aataaaggcg ttgaaatcaa
     4621 cattgaggaa attcttttgg atgatgagaa aactttcaag ctttaccaga ctggtgatac
     4681 aattgggaca ttccagtttg aatctgccgg gatgaggaaa tatttgcagg tactaaaacc
     4741 tacgaacctg gaagatttga ttgccatgaa cgccctttac cggcctggtc ctatggattt
     4801 cattccaaat tacatcaata gaaaacatgg aaatgaaaaa gtggaatatc cgcatgaatt
     4861 gcttgagccc attttgaaaa acacttttgg tataatggtg taccaggagc agattatgca
     4921 aagcgcacaa atacttgcgg gttattcttt agggcaggca gacttgctgc gccgtgccat
     4981 gggcaagaaa aagaaagaag agatggataa gcaaaaagaa atttttgttg cgggtgcgaa
     5041 agaaaaacac ggtattgaga aagaaaaggc agaggagatt ttttccatca tggagcggtt
     5101 tgcccaatat ggttttaacc ggtcacattc tgctgcctac tccgttgttg cttatcaaac
     5161 cacgtatctc aaggctcact ttcctgcgga atatatgtct gccgtgctca ctcacaacca
     5221 ggccaatatt gacaagatta ctttcttcct ggatgaatgt cgtcaccaaa atttggtaat
     5281 tcttggtcct gatattaatg aatctaacat tcattttgat gtaaacaggg agggacaaat
     5341 tcggttcggc atgggggcca taaaaggatc aggagaggcg gcggtggaag caatcatcag
     5401 tgaaaggaat gagaatggaa gctttgcaga tgtttatgac tttgcctcca gagtaaatct
     5461 acgctcggta aataagaaaa cctttgaagc tttggctctc gccggcgcat ttgacagttt
     5521 tgggcaaata caccgccgtc aatatgtaca tgctgaaaat ggagagaatt ctcttataga
     5581 aatggccatc cggtatggta atgcccagca gcaggaaaag aatgcctcac aacaatcttt
     5641 gtttggagcg ggaacaggaa ctgccatttc caaacctaaa gcaccggttt gtgaaccatt
     5701 cggtgagatc gaaaaattga aaatcgaaaa ggagatggtc gggttttata tttccggtca
     5761 tccgttagat caattcagga ttgagatgaa agaatttgtg acttgcccgg ttagagatat
     5821 tgaaaaatat aaaaatcaga aaataaccct ggccggagtt gttactaaat tcactgagcg
     5881 tttcaccaag gcaggcaagc cgtttgggtt gttcaccatt gaagactacg aagactctat
     5941 cgacctggcc atttttggag aagattacct gaaaaaccgg cacatgatga tgctggacaa
     6001 cttcgtatat ctcaccggca aagttgaaga gcggtacaac cagcccggag tatgggaatt
     6061 cagaacatgg aatatccaac ttttgggtga cataaggtcg aatctaagca aagagattat
     6121 tttaagagta ctgctgcaag atattgatga aaatttggtc aggaatctgg ctgagcttgc
     6181 gcaggcaagt cctggaaatt gcaccttaaa gctgaatgta tttgatcctg atgaaaaaat
     6241 tagcctcgac tttctttcca aacaataccg ggtaagtcca agcaaccagt tttttgacaa
     6301 gctagggcgg ttttctgaag ttaagtatga gatcataaag tcgaactaac cttcccgcta
     6361 attttcccat tttctttggg aacaaaatgc tctatatttg cgttctgcca aaagccgggt
     6421 taaatattat atatattatc taaagcgaaa gaatgatgag taaaccgata gaattaaccg
     6481 atgcaaactt cgatgaaatt gtgacgaagt ctgagaagcc cgttcttgtt gatttctggg
     6541 cagagtggtg tggtccttgc aaaatgattc atcccgttgt tga
//
LOCUS       DASWHX010000073         4313 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_424883, whole genome shotgun sequence.
ACCESSION   DASWHX010000073 DASWHX010000000
VERSION     DASWHX010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 4313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4313
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_424883"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2406
                     /gene="porU"
                     /locus_tag="VGA21_05115"
     CDS             <1..2406
                     /gene="porU"
                     /locus_tag="VGA21_05115"
                     /inference="COORDINATES: protein motif:HMM:NF033707.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system sortase PorU"
                     /protein_id="HEX9649918.1"
                     /translation="QNLQTPDIIWDITNPKSPVLQEFDFTGDTGMFGITTDSLHRFIA
                     FNSLLDVRSPDSFTSVPNQNLRSLQTPKLLIITNETFMPEGERLAVFRQSNDGIASEV
                     VLATKIFNEFGSGSPDPTAIRDFVKFLYDKSPDLKYLLLFGRTSYDPKDRISNNTNFV
                     PTYVSRNSLDPLRSYTSDDYFGFLESNEGEWIENTSGDHTVEIGIGRLPVKTPAEAKI
                     VVDKLISYSSDEDFLGKYRNDVYFVGDFGNGDANHHIEQSDQLATLVDTGFVDFNSHR
                     FFLDNFQMLQQPGKNSFPDASKKLTEIVNQGALIINYTGHGGEEGLSNEFLFRTPDLD
                     KWKNPERLPLLVTATCEFGRHDDPIRVSAGELTLIKPNGGSIALVTTARPVFSNTNFE
                     LNKAFYEKVFKKENNQYPRLGDVFMETKNKSLTGPNNRNFSLLGDPSMRLAYPKNNLV
                     IQSINAQPVEFADTLSMLSTVTIDGIITDPNGMELTNYDGTLFVKVLDKVTDLQTLGQ
                     DTQPFVYKAREGIIFSGLSSIVQGKFHFEFILPEDVGFQIGSAKVTMYAQSENTLDDA
                     NGSSLKLIVGGENLTPEVDVTPPSIDLYMEDISFREGGVTGSNTILLAGLEDASGINL
                     LPGQNELIAILDDETELALSEYYEADKDSYMNGWVVLPIDNLDKGFHTIKVYASDVYN
                     NQSVATINFRVENERRLKIENLINYPNPVFDKTTFKFNHNRSGDNLYIQISIISMSGE
                     IVQNLEWQIDNAPTTIDNLTWGDGNFSGKNLTPGVYVGRLLVRSRIDGAKNQAFRRLL
                     IIK"
     gene            2432..3673
                     /gene="porV"
                     /locus_tag="VGA21_05120"
     CDS             2432..3673
                     /gene="porV"
                     /locus_tag="VGA21_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009579256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system outer membrane channel
                     protein PorV"
                     /protein_id="HEX9649919.1"
                     /translation="MNKVFINRRLVTFLLLFSPILSYDVLSQSSQILVGHDTTRRVIT
                     TAVPFLLITPDARSGAMGDAGGAISPDANSIHWNPAKLAFLESEFGLSISYTPWLGNI
                     IKDMSISYLSLYYKITREQAVGFSMRYFDLGDIFFTTDIGDPAGQFSPREFAFDLTYS
                     RMLTENLSIGGSARFISSNLTGSFSSQAIEARPGRSFAVDFGLFYKKDFLLSGTNSNL
                     ALAAVISNIGSKVTYSSEENKDFIPANFRLGTAFTSSLNPYNTITLAVDFNKLLVPTP
                     PIYQIDANGQRVIDQNGEPVILKGKDPDRTVLGGVFGSFGDAPDGAKEEFQEFSIATG
                     VEYWYKELFAVRGGYFLEAENKGNRKYFTLGVGFRYQKFGLDFAYLIPQRQNHPLAET
                     LRFTLVFNFDKAERDTESVVE"
     gene            3673..>4313
                     /locus_tag="VGA21_05125"
     CDS             3673..>4313
                     /locus_tag="VGA21_05125"
                     /inference="COORDINATES: protein motif:HMM:NF012879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEX9649920.1"
                     /translation="MPDRTVAPTFKKLQRIQIPRVTSSRLQNGILLNYINAGTQPVCK
                     IELIFLTGKVHEAKPGISYLTFKMLQEGTRSLSSFQIAEKIELTGSYLGIEPGIEHST
                     IELYFLKNKGSIVIPLLKELFFESTFPEEELKKVKQILSQNLKIELEKTSVVASRLFR
                     KKLFGEMNPYGSFLEPDLISQITQEDLIDFYQTKITQVSLEIIASGLIDAEIL"
BASE COUNT         1325 a          845 c          865 g         1278 t
ORIGIN      
        1 caaaatttac aaacccccga tattatttgg gatataacaa atcccaaaag tcctgtcctg
       61 caagagtttg atttcaccgg cgatacaggc atgtttggaa taacaacaga cagtttgcac
      121 cggttcattg cattcaattc attattggat gtccgatctc cagattcatt cacatcagtt
      181 ccaaaccaga atctcaggtc tttgcaaact ccgaaacttt tgattattac taatgaaacc
      241 tttatgcccg aaggggaaag gcttgcagtt ttccgccaat ccaacgatgg aatagcatca
      301 gaagttgtat tggcgacgaa gattttcaat gaatttggat cagggagccc cgatcctaca
      361 gcgattcgtg attttgtaaa atttctttat gataaatccc ctgatttaaa gtacctgctt
      421 ttgtttggcc ggaccagtta cgatccaaaa gatagaattt caaataacac taattttgtt
      481 cctacatacg tttccagaaa ttcccttgat cctctgcgct cctatacttc ggatgattat
      541 tttggctttt tggaaagcaa tgaaggagaa tggatagaaa atacctctgg tgatcacaca
      601 gttgaaatag gaattggccg cctgcctgta aaaactccag cggaagcaaa aatcgttgtg
      661 gataagctca taagctactc ttctgatgaa gactttctgg ggaagtatag aaatgacgtt
      721 tactttgttg gagattttgg caatggtgat gctaatcacc atattgaaca atccgaccaa
      781 ctggctactt tggttgatac tggttttgtt gattttaatt ctcatagatt tttcctcgat
      841 aatttccaaa tgttacagca acccgggaaa aattcttttc ctgacgcctc caaaaaatta
      901 accgaaatag taaatcaagg tgctttgatt attaattata ccgggcatgg aggtgaagaa
      961 ggtttatcta acgaattttt gttcagaact ccggacctgg ataaatggaa gaatcctgag
     1021 cgactcccgc tcttagttac cgccacttgc gaatttggcc gccatgatga ccccatcaga
     1081 gtttcagcag gtgagttaac cctgatcaaa ccaaatggtg gaagtattgc cttggtaact
     1141 accgcaaggc cggttttttc caatacaaat tttgagttga ataaagcttt ctatgaaaaa
     1201 gtctttaaaa aggaaaataa tcaatatccg agattgggcg atgtgttcat ggaaactaaa
     1261 aataaaagtt taacgggacc aaacaaccgg aatttttcac tgctgggaga tccgagcatg
     1321 aggttagctt atcccaaaaa taatctagtt attcaatcta taaatgccca gccggttgaa
     1381 tttgccgaca ctctaagcat gctttcaacc gtaacaattg atggaataat taccgatcca
     1441 aatggaatgg agctgacaaa ttacgatggc accttgtttg tcaaggtatt ggacaaagta
     1501 accgatttgc aaactctggg acaagataca cagccttttg tctataaagc ccgtgaagga
     1561 ataattttca gtggcttatc atccatagtc cagggaaaat tccattttga atttatttta
     1621 cctgaagatg taggttttca aatagggtcg gccaaagtta caatgtatgc gcaatcagaa
     1681 aatacattgg atgatgccaa cggatcaagt ttaaaattaa ttgttggtgg agaaaattta
     1741 actccagaag tggacgtcac tcctccttcc attgatttat atatggaaga tatctcattc
     1801 cgggagggcg gcgtaacagg ttctaacaca atattattag ccggattaga agatgcgagt
     1861 ggcattaatt tattgcctgg acaaaacgaa ctcattgcaa ttctggacga tgaaacggag
     1921 ttagcacttt cagagtatta tgaagccgac aaagacagtt atatgaatgg atgggtagtt
     1981 ttacctatcg ataatctcga taaagggttc catactatta aagtctatgc ttcggatgtt
     2041 tacaataatc agtctgtagc aaccataaat ttcagggttg aaaacgaaag aagattgaaa
     2101 atagaaaatc ttattaacta tccgaatccg gtttttgaca aaaccacttt taagtttaat
     2161 cataatcgat ccggagataa tttatacatt cagattagca taatttccat gtcgggagaa
     2221 attgtacaaa acctggaatg gcaaattgac aacgcaccaa caactataga taatctgaca
     2281 tggggagatg gaaatttttc cggaaaaaac ttgactccgg gagtatacgt tggcaggtta
     2341 cttgtgcgtt ctagaataga cggggcaaaa aaccaggctt tccgcagatt gctaattata
     2401 aaatgattat ctttaaaccc aattttctac tatgaataaa gtgttcatta acaggaggtt
     2461 agttaccttt ttactgctgt tttctccgat tttatcttat gatgttctaa gtcaaagtag
     2521 tcaaattcta gtaggtcatg atacgactcg tcgtgttata actacggccg tgccattcct
     2581 gctcatcaca cctgacgccc ggtccggtgc aatgggcgat gctggcggag ctatttcccc
     2641 tgacgccaat tccatccatt ggaatcctgc caaactggct ttcctggaat ccgaatttgg
     2701 actttccatc agctatacgc cctggcttgg aaacatcatc aaggacatga gcatctcgta
     2761 tctttcttta tactacaaga taaccagaga acaagccgtt ggcttttcca tgaggtattt
     2821 cgatcttgga gatatctttt ttactacgga tataggagat cccgccggcc agtttagccc
     2881 cagagagttc gcctttgacc tgacatactc ccgaatgctt acagaaaatt tgagcattgg
     2941 cggttcagcg cggtttatca gttccaattt gacaggcagt ttttcctcac aagcaatcga
     3001 agccagacct ggaagaagtt ttgcggtaga ttttggttta ttctataaaa aggattttct
     3061 tctgagtgga acaaactcaa acctcgctct tgccgctgtt atttctaaca ttggttctaa
     3121 ggttacatat tccagcgaag agaataaaga ttttattccg gcaaacttca gattgggaac
     3181 tgcttttaca tcaagcctga atccttataa cactatcacc ctcgccgttg atttcaacaa
     3241 actacttgtt cccacgccac ccatttacca gattgatgca aatggccagc gagtgattga
     3301 tcaaaatggt gaacccgtta tcttaaaagg taaagatcct gacaggacgg ttttgggggg
     3361 agtttttgga tcatttggtg atgcaccgga tggtgcaaaa gaagaatttc aggagtttag
     3421 tattgcaact ggagtcgagt actggtacaa agaattattc gcagtccggg gcggttactt
     3481 tttggaagct gagaataaag gaaaccgaaa atattttacc cttggtgttg gtttcaggta
     3541 tcaaaaattt gggcttgact tcgcttatct aattcctcag agacaaaacc atccccttgc
     3601 agaaactttg cgcttcactc tggtatttaa ttttgataaa gccgaacggg ataccgagtc
     3661 tgttgtagaa taatgcctga ccgtaccgtt gctcccacat tcaagaaact tcaaaggata
     3721 caaataccca gagttacaag ttcaaggctc caaaatggga ttttgcttaa ctatatcaat
     3781 gccggaactc agcccgtctg taaaattgag ttgatatttc ttacaggcaa agtgcatgaa
     3841 gcaaaacccg ggatttctta tcttactttt aagatgctcc aggaggggac aagaagctta
     3901 agttccttcc aaattgcaga aaaaattgag cttactggtt cttacctggg catagaacct
     3961 ggaattgaac attcaactat tgaattatac tttcttaaaa acaaaggatc aatagtaatt
     4021 cctcttttaa aagaactgtt ttttgagtcc acttttcctg aagaagaatt aaaaaaggtt
     4081 aagcaaatac tttcgcaaaa tctaaagatt gagttggaaa aaaccagtgt ggttgcatcc
     4141 aggcttttcc ggaagaaatt atttggtgaa atgaacccat acggctcttt tctggaaccc
     4201 gatttgattt cacaaattac ccaggaggac ctaatcgatt tttatcaaac taaaataaca
     4261 caggtatctc tggaaatcat tgcatccgga ttaattgacg ctgaaatatt aga
//
LOCUS       DASWHX010000074        28966 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_3377744, whole genome shotgun sequence.
ACCESSION   DASWHX010000074 DASWHX010000000
VERSION     DASWHX010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 28966)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28966)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28966
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3377744"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..264
                     /locus_tag="VGA21_05130"
     CDS             <1..264
                     /locus_tag="VGA21_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009579045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEX9649921.1"
                     /translation="ESIVFRDHTFKFIAQGARGYHELAAALLNNKLSYPTVVFLDEDF
                     NMIQPLPGYQQPNTFYPIIEFFGDDHYKKMNWQEFQKIYKSPF"
     gene            327..956
                     /locus_tag="VGA21_05135"
     CDS             327..956
                     /locus_tag="VGA21_05135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649922.1"
                     /translation="MLSYFKPIGLLVARLIIYSLVLVGIAQLLFLDAFNVIDGGKFSE
                     NSYTEWAQVGVLILIILVTFWTGKVSPKEKVIINSMLGFFLMALIREFDFYLDNYVFD
                     GAWQVLAFSTLAVTIIYLVRNKEAIEQASQNVFQTSAFGLISAGILITFIFSRLYGRT
                     SFWEAVMEQKYFRDVKNVSEEGIELLGYGIILIGCIELFRILLNARTAD"
     gene            complement(953..1525)
                     /locus_tag="VGA21_05140"
     CDS             complement(953..1525)
                     /locus_tag="VGA21_05140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649923.1"
                     /translation="MRIFLLILVSNIFITGYPQQLAIYSREDSSLSSQFINAICNATA
                     DKKGVENEPSSLEQLINFAAGTSYKDSSQSKLVYVWWNNHYSDLYCPVGRRDSSHLTR
                     IIVENDLREIANHLGPNGRLSLNLELKDATDSLTLLEFVKQERKSRARKFDFETLRFQ
                     KDKRWKNYTFYMMLFTNYEVEWAVLRGEEN"
     gene            complement(1767..2480)
                     /locus_tag="VGA21_05145"
     CDS             complement(1767..2480)
                     /locus_tag="VGA21_05145"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX9649924.1"
                     /translation="MYPLLQPVKSWITILLSIELQTFGLLELRSYHGGKITGNHFLTI
                     SSTYHMSKEEILTEIDQVIKAQEDPSEFAPIYNRYFDPIFVYVFKRIDDEELTAEITS
                     RVFYNCLYNLKRFKYQGVPFSAWLYKIAINEINSFFRNRNHQTRMVTMSDIHIETIID
                     ELDRSEPEIDKYVLVSTLLEQLTPDEIQFIELRFFENKSFKEVGYFLGISEGNAKIRT
                     YRILEKLRSISKQITYKEV"
     gene            2838..3509
                     /locus_tag="VGA21_05150"
     CDS             2838..3509
                     /locus_tag="VGA21_05150"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9649925.1"
                     /translation="MSKALKKLCIVVGVGPGLGLAIAKRFGKNEFKIAMVARSKSRLL
                     KYQKDLAKYGIDSAPYPCDVGNLKALASAFDNICSEHGNAEVLAYNVSILNPGTPSEI
                     SVDAFVDDFKVNVAGALVAFQKMTPQMKGNKEAAIILTGGGLATDPYFEFASLGAGKA
                     ALRNLAMSMAQEGAKLGIRVATITIKGMIKKGTYFDPEKISLEIWKIYKNKPKRKDAE
                     VVYAK"
     gene            complement(3513..3644)
                     /locus_tag="VGA21_05155"
     CDS             complement(3513..3644)
                     /locus_tag="VGA21_05155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649926.1"
                     /translation="MILEILQTGASAFIILVSVLLAFVAGLSTIDYLKTKENDEQES"
     gene            3786..4046
                     /locus_tag="VGA21_05160"
     CDS             3786..4046
                     /locus_tag="VGA21_05160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649927.1"
                     /translation="MPDDQQTQLLRRVKYLERAIALDRQDYLQKKKSYDAIIDKLMAM
                     VKSGEITSYLCIDSIYYHLLEKIGRGEEDFDKYAAEIRDFVS"
     gene            4216..6342
                     /locus_tag="VGA21_05165"
     CDS             4216..6342
                     /locus_tag="VGA21_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020526116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEX9649928.1"
                     /translation="MKVFDEKHIKNIVLLGAAKSGKTTLAETMLFEAGLINRRGTVEG
                     KNTVSDYQEIEHERGNSVFATALHTEWRDYKINIIDTPGLDDFIGEIISSLRVADTGI
                     MLINSQHGVEVGTELIWNYVEQYEKPTVFVINQMDHPKSNYEQTIESMKARFGSAIIQ
                     MQYPVNQGEGFDSIIDLLKMVMYKFPTDGGKPEKLDIPQDEKEKANALHNELVEKAAE
                     NDEKLMELYFEKGSLDEDEMRQGIKIGMVNHEVFPVFCLSAKNNMGSGRLMGFLDNVA
                     PRSSELNPEETEEGKKLDFDPAKPTSLFVFKTHIEPNLGKLSFFKVLSGTVTTGIDLI
                     NSTTGSHERLNQLFIMDGKSRNPVEKLLAGDIGATLKLKDTETNQTLHTKELPVTVIP
                     IKFPEPRMQMAIRALNKSDDEKLGEVLHKINQEDPTLRINYSKEIKQLIIGTQGELHL
                     AVTQWTLENIYNLHVEFIAPRISYRETIQKTSDSSYRHKKQSGGAGQFGEVYLRVEPY
                     KEGMPKPADLNVRDSQHIELDWGGKLVFNNCIVGGVIDQRFIPSILKGIMEKLEVGPI
                     TGSYVRDVVVSVYDGKMHPVDSNDISFKIAGLMAFKEAFLNANPKLLEPIYELAILVP
                     DEQMGEVMTDLQTRRSIILGMDSKGVYKVIKAKCPLAELDRYTTALRSITQGRASFSS
                     KFIEYAPVPHEIQEKLAHEYHAVEEV"
     gene            6483..6878
                     /locus_tag="VGA21_05170"
     CDS             6483..6878
                     /locus_tag="VGA21_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018478081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="START-like domain-containing protein"
                     /protein_id="HEX9649929.1"
                     /translation="MAPKKRKCVCEFEINASTKMLYPYLYTAGGLAQWFCDDVTVDED
                     KNFNFIWDGNDHKAKLVSHRVNNFVRFEFIPDENENDPSYFELRLEMNELTESVFLKI
                     IDYSDFEDEDELRDLWEGLIGDLKEIVGG"
     gene            6933..8603
                     /locus_tag="VGA21_05175"
     CDS             6933..8603
                     /locus_tag="VGA21_05175"
                     /inference="COORDINATES: protein motif:HMM:NF015684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HEX9649930.1"
                     /translation="MKKIDKLIIRSFIGPFILTFFVVVFILLTIHISKYFEDLVGKDL
                     GWDVLSELLVYFAVFNTPVAFPLAVLLSSLMTFGNLGEHSEIIALKSAGISLTRAMMP
                     IFILVLGITGLAFYTNNYKVPQAALNAYSLIYDIKQKKPALDIKAGTFYNGIPNYSIK
                     VSEKMEDGRTLKDLIIYNHSEGLGNKEVILADSGLMYTILNDRYLKFELYRGTRYLEM
                     SNSNRGQTALVDQFTRTFYDRMEIVLDLSSFDLKRHDKDLFSGDRLTRNFTQLSSDID
                     SLRQDIGKINYDVFRSTKGYFAHHLKDTEGVPLPENLVKYKREMDSLERISGNSPEET
                     HVPERPDVKMLTLPPGKLVPTNQEEKTSTDDALLPLQPARQSLTEKQAAALREYFSEK
                     DKQSLSYNFALNSARQIKSRLTSQNSSLDYQKFQLRKFEIQYNKMLAHSFACIVMFLI
                     GAPLGAIIKRGGLGAPVLFSILFFILFYVIYITGENWAKENAVHIIPAIWMADVLLLP
                     IGLFFLRQARRDARLFDADYYRVMLTRFIKRTANSDESAQIKPEAFVK"
     gene            8657..8932
                     /gene="rpsO"
                     /locus_tag="VGA21_05180"
     CDS             8657..8932
                     /gene="rpsO"
                     /locus_tag="VGA21_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014773232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEX9649931.1"
                     /translation="MYLTQEKKQELFETSGKLKTKTDTGSPESQIALFTYRINHLTDH
                     LQGHKKDHSSRLGLLRLVGKRRRLLDWLQRTDIERYRSILAELNIRK"
     gene            9007..11139
                     /gene="pnp"
                     /locus_tag="VGA21_05185"
     CDS             9007..11139
                     /gene="pnp"
                     /locus_tag="VGA21_05185"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014021149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEX9649932.1"
                     /translation="MLPKVIKKTITLSDGREISIETGKLARQADGSVVVRMGDTMLLA
                     TVVGKSEASEGQDFLPLSVDYQEKFASTGRIPGGFLRREGRLSDHEILVSRLVDRAMR
                     PLFPDDYYFDTQINIFLISGDPKAVPDALAAFGASTALTISPIPFNGPISEVRVARVN
                     GELVVNPSMEGREQADLDIIVGATIDNITMVEGEMDEANEDDLLEALKLAHSIIKEQC
                     HAQLELAKELNVVKREFTPPAGDEDLYKMLYEKYYHRFYEVAGRSLTNKNERRDYFKA
                     IKEEYTASLPEDPEPDIKLIEKYFGKIEKEACRNLMLNESKRLDGRKGNEIRQIWGEV
                     DYLPSAHGSALFTRGETQSLTTVTLGTKLDEQIIDQAMTSGTSKFMLHYNFPGFSTGE
                     VKPNRGPGRREIGHGNLASRAIRKVLPGNDDNPYTIRIVSDILESNGSSSMATVCAGV
                     LALMDAGIKIKNPVSGIAMGMISDGQTGKYAILSDILGDEDHLGDMDFKVAGTSKGIT
                     AVQMDIKVDGLSYDVLKEALIQAREGRIHILGEMAKILDKSREDLKPHAPRSVRIKIE
                     KDQIGAVIGPGGKVVQDIQNTTGATVIIEEVAGGGEVTVFSTNKESMEAAVARIKQIV
                     AVAEVGETYTGKVKSVMPFGAFVEFLPGKDGLLHISEIKWERLEKMDGVLEIGEEIPV
                     KLIGVDKKTGKYRLSRKALLPKPEEKNS"
     gene            11231..12094
                     /locus_tag="VGA21_05190"
     CDS             11231..12094
                     /locus_tag="VGA21_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014796981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HEX9649933.1"
                     /translation="MRQLKISKQITNRESQSLDKYLQEIGKVDLLTPDEEVDLAKRIR
                     DGDQLALEKLTKANLRFVVSVAKQYQNQGLSLGDLINEGNLGLIKAAQRFDETRGFKF
                     ISYAVWWIRQSILQALAEQSRIVRLPLNRVGSLNKISKTFSELEQKYEREPSPDELAE
                     VLEVTTAEVVDTMKISGRHVSMDAPFISGEENSLLDVLENEMEETPDSALMNDSLRKE
                     VQRALSTLTQREADVITLYFGLNGEHSMTLEEIGEKFNLTRERVRQIKEKAIRRLRHT
                     SRSKALKPYLG"
     gene            12184..13125
                     /gene="trxB"
                     /locus_tag="VGA21_05195"
     CDS             12184..13125
                     /gene="trxB"
                     /locus_tag="VGA21_05195"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017729876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEX9649934.1"
                     /translation="MGEKVKVAIIGSGPAGYTAAIYAARAGLNPVLYTGGQPGGQLTI
                     TNDVENFPGYPDGINGPQMMIDLQKQAERFGTKVKYAMVTSVDFSGYPLKLILDEKEE
                     VLAESVIISTGASAKYLGLPSEEKFANKGVSACAVCDGYFFKGMEVAVVGGGDSAAEE
                     STYLSKLCPKVYLIVRRDELRASKIMQDRVLNTPNIEVLWNTETEEISGRESVESIRV
                     VNNKTGEKKEIPVQGFFLAIGHTPNTEIFRDYIELDATGYIKVKPGTTKTNVEGVFAT
                     GDAADKVYRQAVTAAGTGCMGALEAEKFLAAKEMEVA"
     gene            13137..13853
                     /gene="bshB1"
                     /locus_tag="VGA21_05200"
     CDS             13137..13853
                     /gene="bshB1"
                     /locus_tag="VGA21_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017729874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis deacetylase BshB1"
                     /protein_id="HEX9649935.1"
                     /translation="MKLDILVFASHPDDAELGCGGTILSEVKKGKKVGIVDLTLGELG
                     TRGNPDLRKKETAKSSKVLGIAIRENLQFRDAFFINDESHQLEIVKVIRKYKPDIILA
                     NAIQDRHPDHPKAAQLVSQSVFVSGLLKIETEIDTGKQEAWRPSKVYHYIQSWHIKPD
                     FIINISDFWEKKMEAIKCFESQFYSRESAEPETFISSPEFLKLLEARAKEYGSLIGVE
                     FGEGFTVERTPGISALEHLI"
     gene            complement(13845..15080)
                     /locus_tag="VGA21_05205"
     CDS             complement(13845..15080)
                     /locus_tag="VGA21_05205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649936.1"
                     /translation="MDGVTYIEKFSSPEKLIHQLPDENLGIAVTIPSYREEKIKDAIL
                     SLINCDSPPCGVEVLVLINFPDNLEEPDKELHRKQSKELKIWSKKFDNQNLKIHIIEK
                     ELPEKNAGVGLARKLLMDEVVRRFAILDKEGVIASYDADCTCDKNYLVALEIFFANNK
                     SSPGCSIYFEHNLEESPIPSAILEYELYLRYYVQGLRYSTYPHAFHTVGSAMAVRTQS
                     YRMQGGMNMRKAAEDFHFLHKIIPIPGFRNLTTTRVIPSARISNRVPFGTGKAMLNWE
                     SKNMRFWPACKAETFDLIKDFLEQSQSFYKEKITRNWIEKNISPMFHEFLWKENFIEE
                     IELIKSNTKSANTFGKAFLRWFNGLRVLQLTHHLRYNHCGEVPVNEASLNLLKMMERE
                     FSGDNRELLEYYRVLDKSN"
     gene            complement(15083..15730)
                     /gene="pdxH"
                     /locus_tag="VGA21_05210"
     CDS             complement(15083..15730)
                     /gene="pdxH"
                     /locus_tag="VGA21_05210"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013452382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HEX9649937.1"
                     /translation="MDEQDIGKIRREYSSSRLDENSVSEDPFVQFKEWMQDAIKSNII
                     EPTAMNLATATIDGRPSSRIVLLKETSGIGFVFYTNYQSQKGLEIEQNPFGALNFFWV
                     SLERQVRIEGVIEKISFEESDAYFRERPRGSQIGAWTSPQSQRIANREILEQRNQEME
                     KRFHDKEVPRPAQWGGYILVPNMFEFWQGRENRLHDRIQYTLQKDNNWLINRVAP"
     gene            15812..16396
                     /locus_tag="VGA21_05215"
     CDS             15812..16396
                     /locus_tag="VGA21_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020527981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-(ms[2]io[6]A)-hydroxylase"
                     /protein_id="HEX9649938.1"
                     /translation="MKLSIELVAGSKKDWLKAVLDDFDSFLQDHANCERKASAMALSF
                     VAKYPDRREIIPELIETAIEELEHFQSVYAIMTKRGISLPGEMGQDYYIRKLLEYCRN
                     GRNERFLDRLLLASIVECRGAERFRLIYENIEDPDLKDFYHNLWASEAKHGNIFVKMS
                     LLYFNKSEVYARLGELNEIESQVLDTLPVKAALH"
     gene            16399..16980
                     /locus_tag="VGA21_05220"
     CDS             16399..16980
                     /locus_tag="VGA21_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017729885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HEX9649939.1"
                     /translation="MNEYFDYKNNVLPQKGRLLISEPYLLDPNFERTVILLCEHNEEG
                     SFGFVINKPSSLKFEEVVEGVSGFNENLYVGGPVQQDTLHFIHCEPELVEGGIEIDKG
                     LFWGGDFEKILTLIDIKKVDSERFRFFIGYSGWSPGQLEKEIEENSWIVSPGLKADLL
                     FTANSDDLWKLALNQLGGRFKIYSNYPVNPRLN"
     gene            17017..18981
                     /locus_tag="VGA21_05225"
     CDS             17017..18981
                     /locus_tag="VGA21_05225"
                     /inference="COORDINATES: protein motif:HMM:NF015926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF349 domain-containing protein"
                     /protein_id="HEX9649940.1"
                     /translation="MNSERNIIEELDKSETGESVQSENSDQGKEAESNPNAHQPVMEK
                     GGSENSKKDDTAPELDNQVNSDERPEEDHEEHDEYAHDDHESESINYSKLTKPEILQL
                     LEDLEKSPDLMAADKVANFLKSRMGDIIDRERSDALEKFIDEEGSESEFEFPQDKVSI
                     KFEATYRLLKDRKRKYLRELDQVKEKNHEKAIQTLEKLRQFVDSDESSSSFSAFKDIQ
                     KVWKEIGPLPKGQVKNLWANYNALVNLFYDKRSIYFELKELDRKKNLEAKLELCQKAT
                     QLAKDGNLKKAIIELNDLHNEFKHIGPVPPEEQESLWKRFKSASDEIYARRKEHYQQF
                     KAVQKDNLVKKDQLIEKMKSFTEFSSELIKDWNHKTKEILQIQKEWEATGGVPRENSK
                     DINKHFWSHFKEFFKNKNRFFKELEDKREENLKAKEDLVGKVQELISSKDLEKTADQI
                     KSFQKKWKEVGPVPEKKRQQVFDKFKAACDEFFDKRRSSHKEALKQYEANLERKKEII
                     SEIKRMAAEKSSDLTKLDQLKQEFDQIGFVPRNAINSTKSEFSKAVENFIRNANIEDD
                     KKEEILIEAEFSQIKNDPQSVRKLHQKEQSLRRQIYQVENDINLMKTNMEYFAESRNA
                     RLLKDQFNEKITTATDELTRLKKQLRILRS"
     gene            complement(18994..19752)
                     /locus_tag="VGA21_05230"
     CDS             complement(18994..19752)
                     /locus_tag="VGA21_05230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649941.1"
                     /translation="MRKLKNIFLLCLVFALIECDSKVQTKKDENPAVTIIRNSIIAHG
                     GYDLWIQMEELDYDKSIIMYDSSGEVESKIIQIHNYRFKPEFGGNITWSTDHIYRITY
                     DDVGALKIVDNVPQLDSASIFEASVTYLSSHYVLFQPWRLFDPGANLRYVGKDQLDDT
                     TVVDVVKVTFNIGVEPGAEHDIWWFYFDEETSLLSGYLVDHQGRISYIRNESYDSTMD
                     LVFPHHRYSHFVTDTARNNPLLRAEYFYDNFAVR"
     gene            complement(19909..20391)
                     /locus_tag="VGA21_05235"
     CDS             complement(19909..20391)
                     /locus_tag="VGA21_05235"
                     /inference="COORDINATES: protein motif:HMM:NF015999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HEX9649942.1"
                     /translation="MPGVSIDEFLNKHQIEFKLHEHPAVYTTEEATVHTSHIPGLDCK
                     NLLVKDQKDKRFFLLILPASEKADLKEFASKVGEKRLSFANSKNLVDLLGLEPGSVSP
                     FGLINDQDKIVELYIHKKIFDAAIIKFHPNRNTASLEISNAMFRKYLAIIGHEVQIIA
                     "
     gene            complement(20422..23280)
                     /locus_tag="VGA21_05240"
     CDS             complement(20422..23280)
                     /locus_tag="VGA21_05240"
                     /inference="COORDINATES: protein motif:HMM:NF027232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649943.1"
                     /translation="MKNLFSVILLFIAIPCVSQKSPENLSNVYSWRNVGPANQGGRVV
                     DIEAIENDFQKVYVATGSGGVWYSDNAGTTWTPIFDNYSTASIGDIAIYQQDPSIIWI
                     GTGEANNRNSVSWGDGIFKSIDGGKSFQNMGLNSTHQIARVITIPDKPDEVCVCAIGH
                     LWGESGDRGLFKTADGGKTWSKLNNGLPADNKTGCNDLVIDPANPQIMYVSFYQRLRR
                     PWTFHSGGPNGGIFKTTDGGKSWKKLTNGLPADSTGRIGLAIYQQNPKIVMALVEAKR
                     TDTLAIPGSGVYRSEDGGESWKYVNTYNNRPFYYSQIRINPLNDLRVYLLTTRFMVSD
                     DGGKNLRNGSADEEVHGDFHALWLDPTHADRYYLGADKGFSMTHDHGKKFILFDNLPI
                     AQYYRISFDYRDPYYVYGGLQDNGFYATASFSRDIRGILNDSNWKVHWGDGMFTEVDP
                     DDWRRVITSSENGGINKYDPVTHRIESLRPTPLNVTNASQFLNAEQMTNRNPFRFNWS
                     APIQMDHKSKILYLAGNHILKSTNWGESWEIISPDLTTNDPEKTKVGASGGLTPDNSG
                     AETHCTVVTLAISPLDENILWAGTDDGLVHITRDGGKSWTSLRKNFKGVPEGIWVSRI
                     EASQFKKERAYLSFDGHRSDHNKPYLFVTENGGKNWTSITANLPQTEVIRVVRENPVN
                     ENLLVIGTETGIWATIDRGKNWFRFMPGMPTVSVYDLAFHPRDHDLIAGTHGRSLWIM
                     DNMHALEQLTAEVQQKEFYLFDQPVATLWENTSRGGQRGHFWYGGENPEEIRPTSSLP
                     RARYEVDALLYYYLGDIDTSAKAEIIISNVKGQKITRPVKVVRGINKFKWDREFDSEP
                     YTEEQQKTLDAIFEVLIQTNPGMANNNIYQRYLNAQNYRQKRANVQFINRIFNLGIDD
                     SYGIPTGEAGTYEVVLKVDGKEERKILTIREDPILKEN"
     gene            23518..24087
                     /locus_tag="VGA21_05245"
     CDS             23518..24087
                     /locus_tag="VGA21_05245"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX9649944.1"
                     /translation="MNEQLAAAINGFGVFTSSEITQVLDRMHLFPVERNAILLELGQT
                     CQAFHFIQSGSFRHYCPEDGGDTTLNLWTEQQWALDYQSFAAQKPSRNIIRAMEDSEV
                     YELSVYQLHHLIELSPAFFQLGKIMGSAIPDDEILNVKLSPVDKYRALLDKNPKLLQR
                     FALKHIASYLGITPETLSRVRKKMIEQIS"
     gene            24152..24796
                     /locus_tag="VGA21_05250"
     CDS             24152..24796
                     /locus_tag="VGA21_05250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649945.1"
                     /translation="MPAQHYKASGISLIIGSLLMVVTMILHPSGGSIEQILRIINLAI
                     ISHSLAILSTPFLLLGFWGLTNKLKESYFFAMSAFMAMATGLIAVMIAAAVNGIALPL
                     YAQHYAGAPEKTLEIVRHIMAYGTVFNHAFDYIFIGATCLAVLFWSISILDTKALPQW
                     LAYLGIIYFLVCGVMFFMGFSFLYLHSFTVYIFGSVVWILLAAFSLLKMKSNSV"
     gene            25119..26096
                     /locus_tag="VGA21_05255"
     CDS             25119..26096
                     /locus_tag="VGA21_05255"
                     /inference="COORDINATES: protein motif:HMM:NF025679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4382 domain-containing protein"
                     /protein_id="HEX9649946.1"
                     /translation="MWRCKVANQLMGILSVSGMLLFFTSCDYLKDSKEPRGVATLEIT
                     DAPVDDANVNGVYITISEIKVDGKVFAGFENKMTINLLDYQNGSTKLLGTGNVDAGNH
                     KTITLVIDTEVDENGDSPGSFVLTNDLTKHKLISNAQNDIEIDLQKEFVIDASGSSKI
                     VIDFDLRKTIRHTLSNESSDFEFVSEVELENSIRVINHLNSGTIRGTYDGGVFQTSGK
                     VVAYVYKKGEWNPESEIFIQSANELRFKKAVNSSAVDASGNYRLPFLDAGEYEIHFVS
                     YEKNGADDRMNFSSLFEVSSLTQGISPTSVTVDAQSEATLNVIVIGKII"
     gene            complement(26188..26628)
                     /locus_tag="VGA21_05260"
     CDS             complement(26188..26628)
                     /locus_tag="VGA21_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008463838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEX9649947.1"
                     /translation="MFQKIIHTGNSKTTIIIRLMVGAVFLSEGIQKFLFPASHGAGRF
                     EKIGLPAPEFLGTFVGTFEIICGVLLLIGLTTRLASIPLIIIMLVAIATTKIEILANQ
                     GFWEMFHASRTDWSMLLGSIFLLIKGSGNWSMDYKLKNSRPDRL"
     gene            complement(26630..27121)
                     /locus_tag="VGA21_05265"
     CDS             complement(26630..27121)
                     /locus_tag="VGA21_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008463842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649948.1"
                     /translation="MRTDFEVFIFRLTLLILVQSCGLSNQEDSQGNWIKVNEKEKIEI
                     IERQFRGFDNAMVETGYRYQELYWAGQDENWEYAAYQLEKIKVAFENGLQRRPKRAQS
                     AANFLNQALPEVQKSVAIKNTVIFNENFQLLTTHCNSCHAKERVPFFTVKVPTERQSP
                     IRK"
     gene            complement(27372..>28966)
                     /locus_tag="VGA21_05270"
     CDS             complement(27372..>28966)
                     /locus_tag="VGA21_05270"
                     /inference="COORDINATES: protein motif:HMM:NF024962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SprB repeat-containing protein"
                     /protein_id="HEX9649949.1"
                     /translation="FQWNDHASTAIAAAEGLSAGTYEVIGTDARNCVVSFQVVIVDNP
                     DPELNLVEIHPSACGRALGFASVQASGGTPPYSYAWNDGAHQTTPSATHLLYGFYTVT
                     AYDANGCPTNTLNVEVEEIEDLEIIVSEIQNAGCFEGSDGMVRVSVVNGVAPFKFSVD
                     GGIEQTDNEFAGLGVGTHFLKVTDANFCTDETEVIIQAPDELRFAGVSKYNPLCFGVC
                     DGEISVLVAGGVGPYNYLWDTSDITKSVTELCPGNYSVTVTDANGCSVSTTIEILQPA
                     KIHGAGIPEKLIICEGNSVEVDAGLWINYEWTGNNFKSFNRKINIHNPGDYYLQVVSP
                     EGCIVLDTVTLELSNEILKAKALVPSKAFIGDSVEVIDVSWPPADNIEWFLPENVNLL
                     EQGDDRILIKFMEAGIYVVGMDATSGLCQDYVEYEIEIMEGEAVDEGGRLGYEAVIES
                     FSLFPNPNNGRFTAIVGLYRNADITLRVVDNVRSRIIREEDYAGKKEYEIEFSFDDLP
                     GGVYTLNLEVEGEMKNLRFVVN"
BASE COUNT         9082 a         5528 c         5869 g         8487 t
ORIGIN      
        1 gaatctattg ttttcagaga ccacaccttt aaatttattg cacagggtgc aaggggttat
       61 cacgaacttg cagctgccct attgaataat aaattaagtt accccacagt tgtatttctt
      121 gatgaagact ttaacatgat ccagccgcta cccggctatc agcagccaaa tacattttac
      181 cctatcattg aattctttgg agacgatcat tacaagaaaa tgaactggca ggagttccag
      241 aaaatttata agtctccttt ctaaaacaat ttgattattt ctggctgatt tagcattagg
      301 tcgtaaatta cgtcttaatt tgacttatgt tatcttattt taaacccatt ggtctgcttg
      361 tagcaagact tattatttac tcgctggtgc tggttggcat tgcgcaactt ttatttctgg
      421 acgcattcaa tgtaattgat ggtggcaagt ttagcgagaa tagctatacc gaatgggctc
      481 aggtaggagt tttgatattg attatcctgg taaccttctg gacgggaaaa gtatccccaa
      541 aagaaaaagt tattatcaat agcatgcttg gatttttttt gatggcactt atccgggagt
      601 tcgattttta cctggataat tatgtatttg atggcgcttg gcaggttctg gcattttcaa
      661 ctctggctgt tacaataatt tatctggtta ggaacaaaga ggcaattgaa caagccagcc
      721 aaaatgtttt tcaaacttca gcatttgggc taatcagtgc tggtatttta atcacattca
      781 tattttccag gctgtatggt agaacaagct tttgggaagc tgtaatggag caaaaatatt
      841 tccgtgatgt aaaaaatgtt tcggaagaag gaattgaact cctgggttat ggaataattt
      901 taatcggctg cattgaactt ttccgaatct tacttaacgc cagaacagct gattaattct
      961 cttccccacg taaaactgcc cattcaacct cgtaattagt aaaaagcatc atataaaagg
     1021 tataattttt ccaccgttta tctttttgaa agcgaagagt ttcaaaatca aatttcctgg
     1081 ctcttgattt cctttcttgc tttacaaatt caagtaatgt gagactgtct gtcgcatctt
     1141 ttaattcaag attcaggctt agcctgccat taggacccag atgattggca atttccctga
     1201 gatcattttc aacaatgatt cgcgtcaggt gcgaactatc tcttcgccct acaggacaat
     1261 agagatcgga ataatgatta ttccaccaaa cataaaccag ttttgactga ctggagtctt
     1321 tataagatgt ccctgcggca aaattaatta gctgctccag tgaactgggt tcattttcaa
     1381 cccccttttt atcagcggtg gcattacaga tcgcgtttat aaattgagaa gataatgaac
     1441 tgtcttctcg ggaataaata gctaactgtt gtgggtatcc tgttataaaa atatttgaaa
     1501 ctaatatcag caggaatatt ctcaaaaccg ttagaatgta gccaggttac tttcagttag
     1561 taataacatt aataacagta aaacaattat tacaacaaag actaatttct tgatatcata
     1621 tcgaaataaa tatcgaatac cactcggagt atgaaacttc ttatacccgt ctataaagtg
     1681 gtcgaaattc ttatacttac caattaaatt aggtgtggga ccagtatcgt taaattttac
     1741 cttaagttga tgcatttatt ttcctcctat acctccttgt acgtaatttg tttacttatt
     1801 gatctaagtt tctccagaat tctgtacgtc cttattttag catttccctc ggaaataccc
     1861 aggaagtaac cgacttcctt aaaagattta ttttcaaaga accgcagctc gatgaattga
     1921 atttcatcag gagttaactg ctccaataat gtacttacca atacatactt atcaatttca
     1981 ggttcacttc gatccaattc atcaattata gtttcaatgt gaatgtcact cattgtaacc
     2041 atccgtgttt ggtgatttct attcctgaaa aaagagttaa tttcattaat ggcgatctta
     2101 tacaaccatg ctgaaaaagg gactccctga tacttaaatc gcttaagatt ataaaggcaa
     2161 ttgtaaaata ctctggaagt aatttcagcc gtcaattcct cgtcatcaat cctcttgaaa
     2221 acataaacaa aaattgggtc aaagtacctg ttataaatgg gtgcaaactc actgggatcc
     2281 tcctgggctt taatgacctg atcaatttct gtcaggattt cttccttcga catatgataa
     2341 gttgatgaaa tcgtaaggaa atgatttcca gtaattttac caccatgata tgaccttaat
     2401 tcaagcaagc cgaatgtctg aagttcgatg ctaagaagaa ttgttatcca ggatttaacc
     2461 ggttgtaaaa gaggatacat tttttggttg ttttttagga aaatacctga acgccatttg
     2521 ctttttcccg gggattacag tggcacttcc accattccct caattacctc gccacctttt
     2581 gtttcagcaa atcaagcttt caaaccttga tcgaaatagg taaatttatc cggcacaata
     2641 ttatccaccc gcaagggcaa cattgccgtt agtaccaggc attttaaacc gatctcttta
     2701 aactaatcat cttttgtctt tgacttgctt ttgtgtcagc cattttcaat taatttttac
     2761 cctcataatg gcatattatc aaaaagatac aggtttgcag agtaaaaaat tgaaaaacca
     2821 ttaaattggc gcaaattatg agtaaggcac tgaagaaatt atgcatcgtt gtgggagttg
     2881 gtccaggttt gggactggcg attgccaaga ggtttggaaa aaatgaattt aagattgcta
     2941 tggtggcaag atcaaagtcc agacttttga agtatcagaa agatttggca aagtatggaa
     3001 ttgattcagc tccatatcct tgtgacgtgg gaaacctgaa agctctggca agcgcatttg
     3061 ataatatttg cagcgaacat ggcaacgcgg aagtgttggc gtataacgtt tccattttga
     3121 atcccggcac tccttcagaa atttccgttg atgcatttgt ggatgacttt aaagtgaacg
     3181 tggccggggc actcgtggct tttcaaaaaa tgactccaca aatgaaaggc aacaaagaag
     3241 cagctattat tctgacaggt ggcggattgg caaccgatcc ttattttgaa tttgcttctt
     3301 tgggtgcagg taaggctgct ttaagaaatt tagctatgag tatggcgcag gaaggggcta
     3361 agctgggcat aagggtagcg actattacga taaaaggaat gattaaaaaa ggaacttatt
     3421 tcgaccctga aaaaatttcg cttgaaattt ggaaaattta taaaaacaaa cctaaaagaa
     3481 aagatgcgga agtagtctat gcaaaataag cattaagact cctgctcatc attttctttg
     3541 gttttcagat aatcgatagt gctgaggcct gcaacaaatg ccagcaacac acttactaat
     3601 atgatgaaag cacttgctcc agtttgtaaa atctctaaga tcatctgtta ttgcattagg
     3661 taaacccaaa tgtaggtttc ccactgaata aaaaaaacca ttttgcaata gttattatct
     3721 tccggtcttt atttggttca aattgactat atttcgctaa aattggttca cccttatccg
     3781 gttaaatgcc agacgatcaa caaacccagt tactaaggag ggtcaaatat ttggaaagag
     3841 caattgctct ggatcggcaa gattatctcc agaaaaagaa gtcctatgac gctattattg
     3901 ataaactgat ggctatggta aaaagtggcg aaatcacttc ttacctctgc atagattcta
     3961 tctattatca ccttctggaa aaaataggcc gtggtgagga agattttgat aaatatgcag
     4021 cggaaatcag agattttgtc tcctgaaaaa gcgtgtcata tcattaaatc tttccaatca
     4081 aaacatttcc tgtccggcat gccaattctt taccatcagg ttgcatttca aacccgctta
     4141 atataaattt aggagttacc cataaaggga aaactcgaat attccaatgg gttagaaatc
     4201 ttaaattaag cctatatgaa agtatttgat gaaaagcata tcaagaatat agtattgctg
     4261 ggagccgcga aatccgggaa aacaacactt gctgaaacaa tgctgttcga agctggtctc
     4321 ataaacagac ggggaactgt ggaaggtaaa aataccgttt ctgactacca ggaaattgaa
     4381 cacgaacggg gtaattcagt gtttgctaca gctcttcata cggaatggag ggattataag
     4441 ataaatatca ttgatacgcc tggtctggat gattttattg gtgaaatcat ttcctcacta
     4501 agagtagcgg acacaggaat catgctgatt aattcacagc atggtgttga ggttggaaca
     4561 gagctcattt ggaattatgt agaacaatat gaaaagccaa ccgtcttcgt aattaatcag
     4621 atggatcacc ccaaatcaaa ttacgagcaa acaattgaat ccatgaaagc aaggttcgga
     4681 agtgcgatca tccaaatgca atacccggta aaccagggtg aaggtttcga tagtataatt
     4741 gacttattaa agatggtgat gtataaattt cccactgacg gtggaaagcc ggagaaactt
     4801 gatattcctc aagatgaaaa ggaaaaagca aatgcgctcc ataatgaatt agttgaaaag
     4861 gcagccgaga acgatgagaa attaatggag ctttattttg aaaaaggatc actggacgag
     4921 gatgaaatga gacaggggat taagattgga atggttaatc atgaggtatt tcccgtgttt
     4981 tgcctttccg ctaaaaacaa tatgggaagt ggcaggctaa tgggctttct tgataatgtt
     5041 gcccccaggt caagtgagtt aaatccggaa gaaacagagg aagggaaaaa acttgacttt
     5101 gatccggcaa aaccaacttc cttatttgtc ttcaaaactc atatagaacc aaacctcggg
     5161 aaactttcgt tctttaaagt attgtcgggt actgttacta caggaataga tcttataaac
     5221 tcaactacgg gatcccatga aagattgaat caacttttta ttatggatgg aaaatcaaga
     5281 aatccagtag agaaattgct ggccggagac atcggggcaa cattaaaact aaaagatacc
     5341 gagaccaacc agacacttca tacaaaagaa ttaccagtca ctgttatacc gataaaattt
     5401 ccagagccca ggatgcaaat ggcgattaga gctctgaaca aatctgatga tgaaaaattg
     5461 ggagaggtgt tgcacaaaat taaccaggag gatccaactc tcagaataaa ttattccaaa
     5521 gaaataaaac agctaataat tggcactcag ggtgaactgc atcttgcagt aacgcaatgg
     5581 acattggaaa acatttacaa tctccatgtt gaatttatcg cacccaggat aagctataga
     5641 gaaacaatac agaaaacatc agattcatca tatcgacaca aaaaacaatc aggtggtgcc
     5701 gggcaattcg gagaggtcta tttgagggtg gaaccttata aggaaggaat gccaaagcct
     5761 gctgatctaa acgtccgcga ctcgcaacac attgaattgg attggggtgg aaaattggta
     5821 ttcaataatt gtattgtggg tggtgtgatc gatcaacgat ttattccttc tatcctcaaa
     5881 ggtataatgg agaaacttga agtgggacca ataactggtt cttatgtgag ggatgtagta
     5941 gtatcggtgt atgatgggaa aatgcacccc gtggactcca acgacattag tttcaaaatt
     6001 gccggtttga tggcctttaa agaagcattc ctaaatgcca accctaaatt attggaacct
     6061 atctatgaac tggcaattct ggttccggat gaacaaatgg gagaagtgat gacagacctt
     6121 caaaccaggc gttccattat tcttggaatg gatagcaagg gagtttataa agtaattaag
     6181 gccaaatgcc cactggctga attggacagg tatacaaccg ctttgcgaag cattacccaa
     6241 ggacgagcaa gttttagcag taaattcatt gaatatgccc ctgtccctca tgaaatacaa
     6301 gagaaactgg cacacgaata tcatgcggtt gaagaggttt agtctttgaa aagttatgtt
     6361 tggagggata aacaagctcg cgtaggttta aatgagtctg ccaaagagaa attaaataca
     6421 ggaagtacct ttggcttatt ttcaaaaaat gatatattaa aaatcctttt aaagaagtat
     6481 taatggcacc aaaaaagaga aagtgcgttt gtgagtttga gataaatgcc tccactaaaa
     6541 tgctgtatcc gtatttatac actgccggtg gcttagccca atggttttgt gatgatgtaa
     6601 cagtagatga agacaaaaat ttcaatttta tttgggatgg taatgaccat aaagcaaaat
     6661 tagtctcaca tcgggtaaat aattttgtcc gttttgaatt tattcctgat gaaaatgaaa
     6721 atgacccgtc ttattttgaa ttgcgacttg aaatgaatga actcaccgag tcggttttcc
     6781 tgaaaattat cgattattca gattttgagg atgaagatga gcttcgcgat ttgtgggaag
     6841 gactaattgg agacctgaaa gagattgtag gcggctgatt ttggtttatt tttgtgctat
     6901 tccggcacaa atttgacacc ctaaaagttc aaatgaagaa aatcgataaa cttataatcc
     6961 ggtcttttat tggtccgttt atcctgacct tctttgtagt tgtattcatt ttgctcacca
     7021 tccacatttc aaaatatttt gaagatttag taggaaagga tttgggctgg gatgtcttaa
     7081 gcgaattact tgtctatttt gcagtcttca acacacctgt tgcatttccg ttggctgttt
     7141 tgttgtcctc attaatgacg ttcggaaatc ttggtgaaca ttctgaaata attgcattaa
     7201 aaagcgctgg aatttccctt acacgggcaa tgatgcccat cttcatcctt gtacttggca
     7261 taacgggatt agctttctat acaaataatt ataaagtgcc gcaagccgcg ttgaacgcgt
     7321 atagtctcat ctacgacatt aagcagaaaa aacccgccct cgatattaaa gcaggaacat
     7381 tttacaacgg tattccaaac tacagcatca aggttagtga aaaaatggaa gatggtagaa
     7441 cactgaagga tttgattatc tacaatcaca gtgaaggcct gggtaataaa gaagttattc
     7501 tggctgactc aggattaatg tatactattc ttaatgacag gtacctgaag tttgaacttt
     7561 atcgtggcac acggtatctg gaaatgagta attctaaccg cggtcagaca gcattagtag
     7621 atcagttcac caggacattt tacgacagaa tggagatagt cctggatctt tcttcctttg
     7681 atttgaaacg gcatgataaa gatttgttct caggagacag actgaccaga aatttcaccc
     7741 aattatcaag tgatattgat tcattaaggc aagacattgg aaaaattaat tatgatgtct
     7801 ttcgaagtac caaaggatat tttgctcatc acctgaaaga tactgaaggt gttccacttc
     7861 cggagaacct ggttaagtat aagcgagaaa tggattcttt ggaacgaatt tcaggaaatt
     7921 caccggaaga aactcatgtg ccggagagac cggatgtaaa gatgttaact cttcctcccg
     7981 gtaagttagt accaacaaat caggaagaaa agacgtctac ggatgatgca ttattgccgc
     8041 tacagcccgc caggcaatca ttaacagaaa aacaagctgc ggcgttgagg gaatactttt
     8101 cagaaaaaga caaacaatcc ttgtcgtata acttcgccct taattcagca aggcaaataa
     8161 aatcacgact cacttcacaa aactccagcc ttgattatca aaagtttcaa cttagaaaat
     8221 ttgaaatcca gtataataaa atgctggctc attctttcgc atgtatcgtt atgtttttga
     8281 ttggagctcc tttaggcgct ataattaaaa gaggaggact tggagccccg gtgctttttt
     8341 caatcctgtt tttcattctg ttttatgtta tctatatcac cggagaaaac tgggctaaag
     8401 aaaacgcagt tcatataata ccggccatct ggatggccga tgtcttatta ttgccaatcg
     8461 gtcttttttt cctgcgacag gccagaaggg acgcccggct atttgatgcc gactattata
     8521 gggttatgct aaccagattc attaagagaa cggccaattc tgatgaatca gcccaaataa
     8581 agccagaggc atttgttaaa taatcaagat ggtaatacct ttgcgcttga tttagaggaa
     8641 ataacaaata agataaatgt atcttaccca agagaaaaaa caagagctgt ttgaaacgtc
     8701 tggaaaatta aagacgaaga ctgatacggg ttctccggaa tcccagattg ccctatttac
     8761 ctataggatt aatcatctta cggatcacct ccagggtcac aaaaaagacc actcttcacg
     8821 attaggcctc cttaggcttg taggaaaaag aagaagatta cttgactggt tgcagcgaac
     8881 ggatattgaa agataccgtt caatacttgc cgagctaaat attagaaaat aaggattagg
     8941 gattcaattg aatccctatt ttgttcttct tcgggtaggt gcaaaacacg aaacaaacaa
     9001 aattttatgt tacctaaagt aattaagaag actatcactc taagtgatgg cagagaaata
     9061 tcaatagaga ccggtaagct tgccaggcag gcagatggtt cagtggtagt aagaatgggc
     9121 gatactatgt tgcttgcaac agtagttggc aaatcggaag ccagtgaagg ccaggatttc
     9181 cttccccttt cggtagatta ccaggaaaaa tttgcatcaa caggacggat acccggagga
     9241 tttctaagaa gagaaggccg gctttctgat catgaaatat tggtttcaag attagttgac
     9301 agagcaatga ggccattatt tcctgatgac tattattttg acactcaaat aaacattttc
     9361 ttaatatccg gtgatccaaa agcagtaccc gatgcgttgg ctgcatttgg tgcgtcaacc
     9421 gcgctcacta tttcacccat tccttttaat ggtccgatat ccgaggtccg ggtggcgagg
     9481 gtcaacgggg aacttgtggt taatccatcc atggagggaa gggaacaagc ggacctcgat
     9541 ataattgtcg gtgcaactat tgataacata accatggtag agggagaaat ggatgaggct
     9601 aacgaagatg atctgcttga agcattgaaa cttgcccatt ccatcatcaa agagcaatgc
     9661 catgcacagc ttgaattggc aaaagaactt aacgtagtaa aacgagaatt tacacctccg
     9721 gcaggtgatg aagatcttta caaaatgctc tatgaaaaat actaccatag attttatgaa
     9781 gttgcaggaa gatcgttgac caataaaaat gaacgaagag attattttaa agcaattaaa
     9841 gaagagtata cagcctcgtt gcccgaagat ccggagccag atataaaact tatagaaaaa
     9901 tacttcggga aaatcgaaaa agaagcctgc agaaacctga tgctcaatga atccaaaaga
     9961 cttgatggaa gaaaaggaaa tgaaatacga caaatctggg gtgaggttga ttacctcccc
    10021 tcggctcatg gttcagcatt atttactcgt ggagaaaccc aatcattgac cactgtaaca
    10081 ctgggtacga aattggatga gcagattatt gaccaggcaa tgacttcagg tacaagtaaa
    10141 ttcatgttgc actataactt ccctggattt tctacgggtg aggttaagcc taatcgggga
    10201 ccaggcagga gagagattgg acatggaaac ctggcttccc gtgctataag aaaggtcctt
    10261 cccggtaatg acgacaatcc ttatactatt agaattgtct ctgacattct tgaatcgaat
    10321 ggctcttcat caatggcaac agtttgtgcc ggtgttctgg cccttatgga cgccggaatc
    10381 aaaataaaga accctgtttc aggaattgct atgggtatga tctcggatgg tcagactggt
    10441 aagtatgcaa ttctttccga tatccttggc gatgaagatc atctcggaga catggacttt
    10501 aaggtggctg gtacttcgaa aggaattact gcagtgcaaa tggatattaa ggttgatggt
    10561 ttatcttatg acgtgctaaa ggaagctctg attcaggcca gggagggaag gatccacatc
    10621 cttggtgaaa tggctaagat tttggataaa tcaagagaag atttgaagcc acatgccccg
    10681 cggtccgtca ggataaagat tgaaaaagat cagattggtg ccgtaattgg tccaggtggt
    10741 aaagttgtac aggacattca aaatactacc ggtgcaacag taatcataga ggaggtagcc
    10801 ggcggcggtg aagttacggt tttctccacg aataaagaat ccatggaagc cgccgtggca
    10861 agaattaaac aaatcgttgc ggtggctgag gttggtgaaa cttacaccgg aaaagtaaaa
    10921 tcagtaatgc ctttcggagc ctttgttgag ttccttccgg gtaaggacgg acttttacat
    10981 atctccgaaa ttaaatggga gagattggag aaaatggacg gagtactcga aatcggtgaa
    11041 gaaattccag taaaactaat tggcgtagac aaaaagaccg gtaaatacag attgtcaaga
    11101 aaagccctgc tccctaagcc cgaagaaaag aattcctgaa attaattttt cagaattcta
    11161 tttttagtga cgtaattact tggatttggt ttaaattggt tggagcatat ttttcgattg
    11221 atcgaaaagc atgagacaac tcaaaataag taaacaaatt accaatcggg aaagccaatc
    11281 cctggacaaa tatcttcagg aaattggaaa ggtagattta cttacacccg atgaagaggt
    11341 ggacctcgcc aagagaatca gagatggaga tcaacttgca ttagaaaaac ttactaaagc
    11401 taatttgcga tttgttgttt ccgtggcaaa acaatatcaa aatcagggat tgtctttggg
    11461 tgacttaata aatgaaggaa accttggact aataaaagcc gcccaaaggt ttgacgaaac
    11521 gcgcggattt aaatttattt cttatgccgt ttggtggata aggcaatcca ttttgcaagc
    11581 tctcgctgag caatccagaa ttgtccgcct gccactcaac agggtaggtt cacttaataa
    11641 gatttcaaaa acattttcgg aattagaaca aaagtacgaa agagagccaa gtccggatga
    11701 attggctgaa gtattggaag ttacaacagc tgaggtcgtt gataccatga agatttcagg
    11761 caggcatgta tccatggatg ctccttttat ttccggcgaa gaaaatagtt tgctggatgt
    11821 tttggagaat gaaatggagg aaactccgga ttcagctctt atgaatgatt ctttgcgaaa
    11881 agaagtgcaa agggctcttt caactttaac acaacgggaa gcagatgtca taacattata
    11941 ctttggtctt aatggtgaac actcaatgac cctcgaagaa attggcgaaa aattcaatct
    12001 tacccgcgaa agagtaaggc aaataaaaga aaaagcaata cgcagactaa gacacacttc
    12061 cagaagcaag gctttgaagc catatctggg ttagtactcg tgtgcaaccc atcaagatta
    12121 ttcggttctg ggaactattt caaaagcaaa aacgtaatta tataagttac atttaattca
    12181 aaaatgggag aaaaagttaa agtcgcaata atagggtcag gtcctgcagg ttatacggcc
    12241 gcaatttatg cagccagagc agggttaaat cctgtacttt acaccggagg tcagccggga
    12301 ggccagctta caataaccaa tgatgtcgaa aattttcctg gctatcctga tggaatcaac
    12361 ggacctcaaa tgatgataga tcttcagaaa caggcggaga gattcggtac caaagtcaaa
    12421 tacgctatgg ttaccagtgt ggatttttcc ggttatccat taaagttaat tcttgatgag
    12481 aaagaggaag ttctggctga aagtgtgatt atctcaacag gagcaagtgc caaatacctt
    12541 ggattacctt ccgaagaaaa atttgccaat aaaggtgttt cagcctgcgc agtttgcgat
    12601 ggatatttct tcaagggaat ggaagtggca gtggtgggag gaggtgactc tgctgccgaa
    12661 gaatcaactt atttatcaaa gctttgtcca aaggtttatc tgattgtaag aagagatgaa
    12721 ttgcgagctt caaagattat gcaagataga gtgctgaaca caccaaacat cgaagtgctg
    12781 tggaataccg aaacggaaga aatatcagga cgtgagagtg tagagtcaat aagagtagta
    12841 aataataaga caggtgagaa aaaggaaatt ccggtgcaag gcttcttcct ggccattggc
    12901 catacaccta acacagaaat tttccgtgat tatattgaat tggatgcgac cgggtacata
    12961 aaagttaaac cgggcacgac aaaaacaaac gttgagggtg tatttgctac aggagatgcc
    13021 gctgataagg tctatcggca agccgtaact gctgcaggta ccggttgcat gggagctctc
    13081 gaggcggaga aatttctggc tgccaaagaa atggaagtgg cctaacctca gtacgcatga
    13141 aattagatat cctcgtattt gcttctcatc ccgatgatgc cgaactaggc tgtgggggaa
    13201 ctattctttc ggaagttaaa aagggaaaaa aggttggaat cgtagatcta acactgggtg
    13261 aattaggaac tcgaggaaat cctgacctca gaaagaaaga aacagcaaaa tccagcaaag
    13321 ttcttggaat agcaatacgt gaaaatctgc aattcagaga tgcatttttt atcaatgacg
    13381 aaagtcatca attggagatt gttaaggtta ttcgaaaata taaaccggat attatccttg
    13441 cgaatgccat tcaggacaga catcctgatc atccaaaagc agcacagctt gtatcccaat
    13501 ctgtttttgt ttcgggattg ctaaaaattg aaacagaaat agatactggt aagcaagagg
    13561 cgtggcgccc atccaaagtt tatcactaca ttcaaagctg gcacatcaag cctgatttca
    13621 ttattaatat tagcgatttt tgggaaaaga aaatggaagc gataaaatgc tttgaatcgc
    13681 agttctactc cagggaaagt gccgaaccgg aaacttttat ttcatcgccg gaatttttga
    13741 aactcctgga agcgagggca aaagaatatg gatctttgat cggagtggaa tttggcgaag
    13801 ggtttacggt agaaagaact ccgggaatat cagcacttga acatttaatt tgatttgtcc
    13861 agcaccctgt agtattccag caactccctg ttgtcacctg aaaactctct ctccatcatt
    13921 ttcaataaat tcaatgaagc ttcattaaca ggaacctcgc cacaatgatt atatctcagg
    13981 tgatgagtta actgcagaac ccggagtccg ttaaaccacc ttaagaatgc cttgccaaat
    14041 gtgtttgcgc tttttgtatt gctttttatc aattcgatct cttcgatgaa attttctttc
    14101 cagagaaatt catggaacat aggggaaata tttttttcaa tccaattccg ggtaattttc
    14161 tctttataaa aagattggga ctgttcaagg aaatctttaa tcaggtcaaa tgtttcagct
    14221 ttgcaggccg gccagaacct catgttttta gattcccagt tcagcattgc tttgccggtt
    14281 ccaaaaggaa cccggtttga gattcgtgct gaaggaatta cccgcgtggt cgttaaattc
    14341 ctgaacccgg gaattggaat tatcttatgt aaaaaatgaa aatcttctgc agctttacgc
    14401 atattcattc cgccctgcat cctgtaactt tgagttctaa cagccattgc tgaaccaact
    14461 gtatggaagg catggggata agttgagtat ctcaaaccct gaacgtaata gcggagatat
    14521 aattcatatt caagtattgc agatggaatt ggactttctt ccaggttatg ttcaaagtaa
    14581 atgctgcagc ccggtgaact cttattattt gcaaaaaaaa tttccaaagc gactaagtag
    14641 tttttatcac aagtacaatc cgcatcgtaa gaagctatca caccttcctt gtcgagaatt
    14701 gcaaacctcc tgacaacctc atccattaac aatttccggg caaggccaac gcccgcattt
    14761 ttttccggaa gttccttttc aattatatga attttaagat tttggttgtc gaattttttt
    14821 gaccagattt ttaattcttt agattgcttt cggtgtaact ctttatccgg ttcttctaaa
    14881 ttatcaggaa agttgatcaa gacaagaact tcaaccccgc aaggtggcga atcacaattt
    14941 ataagagata gaatagcatc ttttattttc tcttctctgt aagatggaat tgttactgca
    15001 atccccagat tttcatcagg taattgatga attaattttt caggtgagct aaatttttct
    15061 atgtatgtta ctccatccac cttcagggag caacccggtt aattaaccaa ttgttatctt
    15121 tttgcagagt atattgaatc ctgtcatgca aacggttttc tcgtccttgc caaaactcaa
    15181 acatatttgg gactaaaata tacccacccc attgagccgg tcttggaact tctttatcat
    15241 gaaagcgttt ttccatttcc tggttacgct gctccagaat ttcccggtta gcaattcgct
    15301 gactctgtgg tgaagtccat gcgccaatct ggcttcctct tggtcgctca cgaaaatatg
    15361 catcactttc ttcaaaagag atcttttcaa taacaccttc aatgcgcacc tggcgttcca
    15421 gtgaaaccca gaaaaaattt aatgcaccaa agggattctg ttcaatttcc agccctttct
    15481 gactttgata gtttgtgtag aaaacaaaac cgatgccgct tgtttctttt aataaaacga
    15541 ttctggaaga aggtctccca tcaatagttg cggtagcaag attcatagcc gtaggttcaa
    15601 tgatgttgga ctttatggca tcctgcatcc attctttaaa ttgtacaaat ggatcttcag
    15661 aaacagaatt ctcatctaac ctgctggaag aatattctct tcgaatttta cctatgtctt
    15721 gttcgtccat cagtttttta tctaaattaa ctcatatatt tttatgtgat aaaattttta
    15781 tttgaattat tattagacaa ttaaatttcc catgaaatta agtatcgaat tagttgcagg
    15841 gagtaagaaa gattggttaa aagcagttct agatgatttt gattcctttc ttcaggatca
    15901 tgcaaactgc gaaaggaaag catctgccat ggcattaagt tttgtagcaa agtatccgga
    15961 tagaagagaa attattcccg aattgataga aacggcgatt gaagaacttg aacatttcca
    16021 atccgtttat gcaattatga caaaaagagg gatatcatta ccaggggaaa tggggcagga
    16081 ttattatata agaaaattat tggaatattg ccgtaatggg agaaatgaaa ggtttttaga
    16141 cagactcttg ctagcatcca tagttgaatg cagaggggct gagaggttta gactgatata
    16201 tgaaaatatt gaggatcctg atttgaaaga cttttaccat aatctttggg cctccgaagc
    16261 aaaacatggc aatatttttg taaaaatgtc attgctttat ttcaataaaa gtgaggttta
    16321 tgcacgactt ggggaattaa acgaaataga atctcaggtt ttggatactt tacccgtaaa
    16381 agctgcttta cattgattat gaatgaatat tttgattata agaataatgt attaccacaa
    16441 aaagggagat tattgatttc agaaccctat ttattggatc ctaattttga aagaactgtt
    16501 attctattgt gcgaacataa tgaagagggt tcatttggat ttgtaataaa caagccaagc
    16561 tcactaaaat ttgaagaagt ggtagaagga gtgtccggtt ttaatgagaa tctttatgtt
    16621 ggcggacccg ttcaacagga tacattgcac tttattcatt gtgaacctga attggttgaa
    16681 ggtgggatcg aaattgataa agggctattt tggggaggag attttgaaaa aatattaact
    16741 cttattgata taaaaaaggt tgattcagag cgatttagat tttttatagg ttattcagga
    16801 tggtcacccg ggcaactcga aaaggaaatt gaagagaatt cctggatagt atctcctggc
    16861 ttgaaagctg atttattgtt tacagcaaat tcggatgatc tttggaaatt agcattaaat
    16921 caacttggtg ggaggtttaa aatatattca aactaccctg ttaaccccag gcttaattaa
    16981 tatttttgaa tattgaatgg ttgcaaacca gaagacatga atagcgagcg taacattata
    17041 gaggaactgg ataagtcaga aaccggtgaa tctgttcagt ccgaaaattc ggatcaagga
    17101 aaggaagctg aatccaatcc caatgcccat caaccggtaa tggaaaaagg cggtagtgaa
    17161 aattccaaaa aagatgacac tgcccctgaa ttggataatc aggttaactc agatgaacgt
    17221 cctgaagagg accatgagga gcatgacgaa tatgctcatg atgatcatga atcggagagt
    17281 ataaattaca gtaaacttac caagcctgag atattacagc ttcttgaaga ccttgaaaaa
    17341 tctcctgatc ttatggcggc ggataaagtt gccaatttcc tcaaatccag aatgggtgat
    17401 attattgatc gcgaacggtc ggacgccctg gaaaaattta ttgatgaaga aggttcggaa
    17461 tcagaatttg aatttcctca ggataaagta tccataaaat ttgaagccac ataccggctg
    17521 ctgaaagacc gcaagcggaa atatctgcgt gagttggatc aggttaaaga aaagaatcat
    17581 gagaaagcaa ttcaaaccct ggaaaagctc aggcaatttg ttgattctga tgaaagcagt
    17641 tcaagcttca gcgcgtttaa ggatattcaa aaagtgtgga aagaaattgg tccattacca
    17701 aaaggccagg ttaagaattt atgggcgaac tataatgctt tagttaattt attttatgat
    17761 aagcgaagca tttactttga attaaaggag cttgacagga agaaaaatct ggaagctaaa
    17821 ctggaactgt gccaaaaagc cacgcaactt gccaaagacg ggaacctcaa aaaagccatt
    17881 attgaattaa atgatctcca caacgaattc aagcatattg gcccggttcc cccggaggaa
    17941 caggaaagtt tgtggaaaag atttaaatct gcatcggatg aaatctatgc aagaaggaaa
    18001 gagcattacc agcaatttaa ggcagtccag aaagataacc tggtaaagaa agatcagcta
    18061 attgaaaaaa tgaaatcctt tacggagttt tcatctgaat taatcaagga ctggaaccat
    18121 aagacgaagg aaatcctgca aattcaaaaa gagtgggaag cgacaggggg agtgcccagg
    18181 gaaaattcca aagatattaa taaacacttt tggtcacatt ttaaagaatt tttcaagaac
    18241 aagaatagat tctttaaaga gcttgaggat aagagggaag aaaatcttaa ggcaaaggaa
    18301 gatttggttg gtaaagttca ggaattaatt tcatcaaagg atttggagaa aactgctgat
    18361 cagataaaat catttcagaa aaaatggaaa gaagtagggc cggtcccaga gaagaagcgg
    18421 cagcaggttt ttgataaatt taaagcggct tgcgatgagt tttttgataa aagaaggtcc
    18481 agtcataagg aagccttaaa acaatatgaa gccaatctgg aaaggaaaaa agaaataatt
    18541 tcagaaataa aacgaatggc agctgagaaa tcttcagacc ttacaaaact tgaccaactt
    18601 aagcaggagt ttgatcagat aggatttgtt ccccggaatg caataaactc aacgaagtct
    18661 gaattttcaa aggctgtaga aaattttatc aggaatgcta atattgaaga tgataagaaa
    18721 gaagaaattc ttattgaagc ggaattttca caaatcaaga atgatcctca atcggtcagg
    18781 aagcttcatc aaaaagagca gtctttgcgc cggcaaatat atcaggttga aaatgacatt
    18841 aacctcatga aaaccaatat ggaatatttt gctgaatcca ggaacgctcg tttactcaaa
    18901 gatcagttca atgagaagat aactacagct actgatgaac ttaccagatt gaagaagcaa
    18961 ctccggatac ttcgttcata gaagtagccg caattatctc acggcaaaat tatcgtagaa
    19021 atattcagcg cgcagcagcg ggttattacg ggcagtgtct gtcacaaagt ggctatatct
    19081 gtgatgtgga aagaccaaat ccattgtgct gtcataggat tcatttcgga tataacttat
    19141 ccttccctgg tgatccacca ggtatccgga aagaagtgaa gtttcttcgt caaaataaaa
    19201 ccaccagata tcatgctctg cgccaggttc gactcctatg ttgaaagtaa cttttactac
    19261 atctaccact gtggtgtcgt ctagttggtc tttccctaca taacgtaaat tagctcccgg
    19321 atcaaatagt ctccagggct gaaaaaggac ataatgagag gacagataag tgacgctggc
    19381 ttcaaatatc gatgcgctgt caagctgagg tacgttatct acaattttca atgcaccaac
    19441 atcatcgtag gtgatcctgt aaatgtggtc ggtggaccag gtaatattgc caccaaactc
    19501 cggtttaaac ctgtaattat gaatttgaat tatttttgat tctacctcgc cggaagaatc
    19561 atacattata atactcttgt cataatcaag ttcttccatt tgaatccaaa ggtcatagcc
    19621 tccatgtgcg ataatggaat tccggattat ggtaacagcg gggttttcat cttttttagt
    19681 ttgaacctta gagtcgcact caattaaggc aaatactagg caaagcagaa aaatgttttt
    19741 tagttttctc atggcccaaa aatagttttc gatctttaaa taaacaatta gtgcatagca
    19801 gctttataga atcaacaatt aagaagtctt ttgcttaatt cacctgacgt ttctgaaact
    19861 ggatcataat catttccaat caggcgacgt caaactgtta atcagatgct atgctataat
    19921 ttgaacttca tgaccaataa tagccagata ctttctaaac atagcatttg aaatctccag
    19981 cgatgccgta ttccggttag gatgaaactt tattattgct gcatcaaata tttttttatg
    20041 aatgtacaat tcaacgattt tatcctgatc atttataagc ccgaaaggtg aaaccgagcc
    20101 aggttcaagt ccaagtaagt ccaccagatt tttcgaatta gcaaaactta gccttttctc
    20161 accgactttc gatgcgaatt ctttcaaatc agccttttct gaagccggaa gaattagcag
    20221 aaaaaatcgt ttgtctttct gatctttaac cagtaaattc ttgcaatcca atccgggaat
    20281 gtgagaagtg tgaacagttg cttcttcagt agtatatacg gcagggtgtt catgcagctt
    20341 aaattctatc tgatgtttat tcagaaattc atcaatacta acaccgggca ttattcataa
    20401 tttagtactc ataaccggat tctaattttc tttcaaaatt ggatcctcac ggatagtcag
    20461 aatttttctt tcttccttgc cgtcaacttt taataccacc tcataagttc ccgcttctcc
    20521 ggtgggtatg ccataagaat catcaattcc caaattaaaa attctgttaa taaactgaac
    20581 gttcgctctt ttctgacgat agttttgcgc attcagatat cgctggtaaa tgttgttatt
    20641 agccattccc ggatttgttt gaattaagac ttcaaaaatt gcatctaaag ttttttgctg
    20701 ttcctcagta tatggttctg aatcaaattc ccgatcccac ttaaatttat ttatcccacg
    20761 aactactttc accggccggg taattttttg accctttaca tttgaaataa taatttcagc
    20821 ctttgcagac gtatcaatat cacccaaata ataatacagt aaagcatcaa cctcataccg
    20881 ggcccggggc aaagaagaag tgggtctgat ttcttccggg ttttcaccac cataccaaaa
    20941 gtgtccgcgc tgcccaccac gacttgtatt ctcccaaagg gtggcaaccg gttgatcgaa
    21001 aaggtaaaat tctttctgtt gaacttctgc agttagttgt tcaagggcgt gcatgttatc
    21061 catgatccat aaacttcggc catgagttcc agcgattaag tcatgatctc tgggatggaa
    21121 agccaaatca taaactgaaa cggtgggcat accgggcata aaccggaacc aattcttacc
    21181 acgatcaata gtggcccaaa tgccggtttc cgttccaatt actaataaat tttcattcac
    21241 cggattttcc ctcacaacac ggataacttc tgtctgaggt aaatttgcag taatgcttgt
    21301 ccagttcttt ccgccatttt cagtgacaaa tagatagggt ttgttgtggt cacttctatg
    21361 accatcaaat gaaagatatg ctctttcctt tttaaattgt gaagcctcaa ttcgacttac
    21421 ccagattcct tccggaactc ccttaaaatt cttacgaagc gaagtccatg attttccgcc
    21481 atccctggtt atgtgcacta gtccgtcgtc cgtcccggcc cataaaatgt tttcatccaa
    21541 aggtgaaatt gccagtgtaa caactgtaca atgagtttcg gctccggaat tgtcaggagt
    21601 taagccgcca ctagctccaa ctttcgtctt ttccggatca ttcgtggtta aatccgggct
    21661 aataatttcc cagctttctc cccagttcgt tgatttcaat atatgatttc cagccaggta
    21721 caaaatcttt gatttatgat ccatttgaat tggagcggac caattgaaac gaaaggggtt
    21781 tcggttcgtc atctgttcag catttaaaaa ctgacttgca ttggtcacgt tcaatggtgt
    21841 tggtcgcagg ctttctatcc tgtgggttac cggatcgtat ttattaatgc cgccattttc
    21901 ggaggaggta attacccttc gccagtcatc aggatccact tcagtaaaca taccatcgcc
    21961 ccaatgcact ttccagttac tatcattaag gattcctcga atatccctgg aaaagcttgc
    22021 agttgcatag aacccgttat cttgcagccc accatataca taatagggat cgcggtaatc
    22081 gaaggagatg cgataatatt gagcaatggg caggttatcg aataaaataa atttcttgcc
    22141 atgatcatga gtcatagaga atcctttatc agcgcctaaa taatacctgt cagcatgtgt
    22201 gggatcaagc cataaggcat ggaagtctcc gtgcacttct tcatctgcac ttccgtttct
    22261 gagattctta cctccatcgt cagaaaccat gaaacgtgta gtgagtaaat atacccttag
    22321 gtcattgaga ggatttatgc ggatttggct gtaataaaat ggccggttgt tataggtatt
    22381 tacgtatttc cacgactcgc cgccatcttc cgatcggtat actcctgaac ctggtattgc
    22441 caatgtgtcg gtacgttttg cttcaaccag ggccattaca atttttgggt tctgctgata
    22501 gatggctaat ccgattctgc ctgtactgtc ggcaggtaat ccgttggtta attttttcca
    22561 ggatttacct ccgtcagtag ttttgaaaat acctccattg ggtccgccac tatgaaaagt
    22621 ccagggtctt cgaagacgtt ggtaaaaaga aacatacata atctgcggat tggcgggatc
    22681 gataaccaga tcattgcagc cggttttatt gtcagccggc aaaccattgt tgagtttaga
    22741 ccaggtcttt ccgccgtcag cagttttgaa cagtcctcta tcccccgatt caccccataa
    22801 atgaccgata gcgcaaacac aaacttcatc cggtttgtcc ggaatagtaa ttaccctggc
    22861 aatctgatga gtcgagttta gtcccatgtt ctgaaatgat ttaccaccat caatggattt
    22921 gaaaatgcca tcaccccagg aaacgctgtt gcgattattg gcttccccgg ttcctatcca
    22981 gataatagat ggatcttgct ggtaaatagc aatatcgcct atagaagcgg tggaataatt
    23041 atcaaatatg ggtgtccagg tagtgcccgc gttgtcagaa taccaaacac cgcccgagcc
    23101 ggtggcaaca tacactttct ggaaatcatt ttcgattgct tcaatatcaa ccacgcgtcc
    23161 tccctggtta gcgggtccca cattgcgcca actataaaca tttgataggt tctccgggga
    23221 tttttgagaa acacaaggaa tggcaataaa aagtagaata acagaaaaca ggtttttcat
    23281 acgtcttgaa gttaattagg agagaaggta agtaatatcc ggcaaggaat caattggaat
    23341 tttataagca gcgtcttctt tattctcgaa atccaagttc agcttttatt ttattcttta
    23401 aagaaatacc ttacatcggc atgaaaatta ttcaggaaag gattctcaag ttatccatgt
    23461 ttgcattcaa taatttcatt ttaagtaaag gttttaaaaa attaataact tgtccctatg
    23521 aatgaacaac tagctgccgc aattaatgga tttggtgtat ttacgagtag tgaaattact
    23581 caggtgttgg atagaatgca tttattcccg gttgaaagaa atgcaatcct cctggaactg
    23641 gggcagacct gccaggcttt tcattttatt cagtcaggta gtttccgcca ttattgccct
    23701 gaggatggag gtgacaccac gttaaatctc tggacagaac aacaatgggc gttagactat
    23761 cagagttttg cagcgcaaaa accctcaagg aacattatcc gggccatgga agatagcgaa
    23821 gtttatgagt taagcgttta tcagcttcat catttaatag aactttcgcc ggcttttttt
    23881 caattgggta aaattatggg aagtgccatc cctgatgatg aaattctaaa tgtaaaattg
    23941 agcccggtag acaaataccg ggctttgctg gacaaaaatc ctaaactcct gcagcgattt
    24001 gccctaaagc atattgcttc ttatctgggg ataaccccgg aaacgcttag ccgggtgcgg
    24061 aaaaaaatga ttgagcagat ttcttgattt cgatcaaaac atatcaaaga caatctttac
    24121 tatgtttgat gcttaaacgg ttgaacccaa aatgccagcc caacactata aagcttccgg
    24181 aatttcgtta attattggct cattattgat ggttgtaacc atgattttac atccctcagg
    24241 gggtagtatt gaacaaattc ttcgaataat caaccttgct atcatctctc attccttagc
    24301 aatccttagt actcctttct tattactcgg attctggggc cttacgaata aactgaaaga
    24361 gtcgtatttt ttcgccatgt cggcatttat ggcaatggct accggactaa tagctgtaat
    24421 gatcgctgct gcggtaaatg gaatcgcact tccgctgtat gcgcagcatt atgccggagc
    24481 tccggagaaa actctggaga tcgttaggca tataatggct tacggaactg tatttaatca
    24541 cgcttttgat tatatattta tcggagccac ttgtctggcc gtacttttct ggtccatatc
    24601 cattttagat actaaagcct tgccccaatg gttagcatat ttaggtatca tttatttttt
    24661 ggtgtgcgga gtaatgtttt ttatgggttt ctcattcctt tatcttcaca gtttcactgt
    24721 gtatattttt ggatcagtgg tttggatttt actggccgca ttttcactgt tgaaaatgaa
    24781 aagcaattca gtttagaata caaatcattt ctttcctcaa tcttaattta actgcataaa
    24841 tggaggtcct ttgaaggctg ttctgctaaa gatttaattt tcaattctgg aaaatcttgt
    24901 cgatttgaaa actaatccca tacactttga actgaaattt gccataaact atagaaataa
    24961 aaatagtaaa aaaataagtg gaatcacggt cttgcgtgac tataaggtac tctgaatggt
    25021 aataattcaa tagttctgag actctcgttt aaaactggta tagaatttta gttatctggg
    25081 agaaatactt tcaaccttaa aataatttaa ttatgaaaat gtggagatgc aaagtggcta
    25141 atcaattaat gggtattctg tcagtgtcgg ggatgttgtt atttttcaca tcttgtgatt
    25201 acctgaaaga ttcaaaagaa ccgcggggcg tggcaacgtt agagattacg gacgcaccgg
    25261 tggatgacgc caatgtaaat ggtgtttaca ttactatctc ggaaatcaaa gtggatggaa
    25321 aagttttcgc cggatttgaa aacaaaatga ccatcaacct tttggattat caaaatggga
    25381 gcacaaagtt actgggaaca ggcaacgtgg acgctggaaa ccataagact attactcttg
    25441 taattgatac tgaggtggat gaaaatggcg attcacctgg aagttttgtt ttgactaatg
    25501 atcttacaaa acataaactg atcagcaatg ctcaaaacga cattgaaatt gatttgcaga
    25561 aagaatttgt cattgatgct tcaggctctt ccaaaattgt cattgatttc gacttaagga
    25621 aaaccatcag gcataccctg agcaatgaat caagtgactt cgagtttgtg tcagaagttg
    25681 aattggagaa ttccattcgg gtgattaatc atttgaattc aggtacaatc cggggaactt
    25741 atgatggcgg tgtatttcag acttcaggta aagtagtggc ttatgtatat aagaaaggcg
    25801 agtggaatcc ggaatctgaa atttttatac aatctgccaa tgagctgcga tttaaaaaag
    25861 cagtgaatag ttcagcggtt gatgcctcag gaaattaccg gttaccattt cttgatgccg
    25921 gggagtatga gatccatttt gtttcgtacg aaaaaaatgg cgctgatgat aggatgaatt
    25981 tcagctcttt atttgaagtt tccagtttaa cccagggtat ctcaccgacc agcgttaccg
    26041 tggatgctca gtctgaagca accctcaatg taatagtgat tggaaaaatt atctaacgat
    26101 ttttcttgcc tgttactata tagaacgtcc ttgttatgag catggacgtt cgttttttaa
    26161 cataaacttg ataggttgta tattgaatca caacctgtca ggtctactgt ttttcagttt
    26221 gtaatccata gaccaattac cactcccttt aattaacaga aaaatacttc caagcaacat
    26281 agaccaatct gttcgacttg cgtgaaacat ttcccaaaac ccctggttgg ctaaaatttc
    26341 tattttagtt gtcgcgattg cgaccaacat tatgatgatt aatggaatac tggctaaccg
    26401 ggtggtaaga ccaatcagca aaagtacacc gcaaatgatt tcaaatgtgc ctacaaacgt
    26461 tcccaaaaat tccggtgccg ggagtccaat tttctcaaac ctccctgctc catgactggc
    26521 gggaaataaa aatttttgaa taccttctga aaggaaaacg gcccccacca ttaagcgaat
    26581 gataattgtt gtttttgagt tgccggtatg aataatcttt tgaaacatgc tactttctta
    26641 tgggtgactg tctttcagtt gggactttta ctgtaaaaaa tggaaccctt tcctttgcat
    26701 gacaactatt gcaatgagtt gtcagcaatt gaaagttttc gttgaaaatt acggtatttt
    26761 taatcgctac cgatttctgc acttcaggta aagcctgatt caaaaaatta gcagcggatt
    26821 gagcacgctt tggtctgcgt tgcaaaccgt tttcaaaagc tacctttatt ttctccaatt
    26881 gataggcagc atattcccag ttttcatcct ggccagccca gtagagttcc tggtagcggt
    26941 atccagtttc taccatggca ttatcaaaac ccctaaactg tctttcaata atctcaatct
    27001 tttctttctc atttactttt atccaattac cctgggagtc ctcctgattt gatagcccgc
    27061 acgattgaac caggattaat aaagttagcc ggaaaataaa tacttcaaag tcagttctca
    27121 ttgttacaag ttagcttttt ttaataggag gaagaaactc accgagtcaa acctgacaag
    27181 tttatcaaga acatcaagtc aatttacatc ctgaagccaa acttgtcagg tttttttagc
    27241 atcgagtcaa tttacctccc gaattaaact tatcaataat taaattccaa ggaacattaa
    27301 acctgacaag tttatcaaga acatcgagtc aatttaactc ctgaagccaa acttgtcagg
    27361 ttttaaatgc ttcaattcac tacgaacctc aaattcttca tctcaccttc tacttccagg
    27421 ttgagggtat atactccgcc aggtaggtca tcaaaggaaa attcgatctc gtactctttc
    27481 ttaccagcgt aatcttcttc cctgataatc ctgctccgga cattatccac cactctgagg
    27541 gtgatgtctg catttctata taatcccacg atggcagtaa accgtccatt attcgggttg
    27601 ggaaataatg aaaaagactc tatcaccgct tcataaccca gccgtccacc ctcatcgacg
    27661 gcctctcctt ccatgatttc aatttcatac tctacgtaat cttggcataa tccggaggtg
    27721 gcatccatcc caactacgta aattcctgcc tccataaatt ttatcaggat gcgatcatct
    27781 ccctgctcca gtaaattaac attttcaggt aaaaaccatt cgatattatc tgccggtggc
    27841 caggaaacat caattacttc cacagaatcc ccaataaatg ctttgcttgg aaccagggct
    27901 ttggctttta gtatctcatt ggaaagttcc agtgttaccg tatccaggac aatacaaccc
    27961 tccggtgaaa ccacctgcaa ataataatct ccgggattgt ggatatttat tttccggtta
    28021 aaggatttga aattatttcc ggtccattcg taatttatcc acaaaccagc gtctacttct
    28081 acactgttgc cctcacaaat gatcaatttc tccggaattc cagcaccatg aatctttgca
    28141 ggctgtaaaa tttcaattgt agtagataca ctacatccat tagcatctgt aactgtaact
    28201 gaataattac caggacatag ttcagtcaca gattttgtta tatctgatgt gtcccataaa
    28261 taattgtacg gaccgacgcc tcctgcaaca agaacagaaa tctcaccatc acaaacacca
    28321 aaacaaagtg gattatattt agaaacacca gcaaacctta gttcatccgg cgcttggata
    28381 attacctctg tttcatcggt acaaaaattc gcatcggtca cctttaagaa atgagtgcca
    28441 acgcctaacc cggcaaattc attgtccgtt tgttctatgc caccatcaac agaaaatttg
    28501 aatggtgcaa caccatttac aacacttacc ctaaccattc catcactccc ctcaaagcaa
    28561 ccggcattct gaatttctga aactattatt tctaaatctt caatctcctc tacctctacg
    28621 ttcagggtat ttgtcgggca gccatttgca tcgtacgcgg taactgtata gaaaccataa
    28681 agtaaatgag ttgccgatgg cgttgtctga tgcgccccat cattccatgc atacgaatat
    28741 ggcggagttc cccctgaagc ttgaacggaa gcaaatccca aagcacgacc acaagcgctc
    28801 gggtgtattt ctaccaaatt caattctgga tcaggattat ctactataac cacctgaaaa
    28861 ctcaccacac aattacgtgc atcagtgcct atcacctcgt aggtgccggc cgataaacct
    28921 tccgcggccg caattgcagt acttgcatga tcattccatt gaaaca
//
LOCUS       DASWHX010000075         2033 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_1537200, whole genome shotgun sequence.
ACCESSION   DASWHX010000075 DASWHX010000000
VERSION     DASWHX010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1537200"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..552
                     /locus_tag="VGA21_05275"
     CDS             <1..552
                     /locus_tag="VGA21_05275"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX9649950.1"
                     /translation="YELKSKVIDNQPVHDEDEVYFIVSGKAKLVAGDSTFDVSAGSIA
                     FVRAGVSHKFVEIEEAFSALVIFTKTPTKTGDADALAFELDKLRQPRNPEENVWNQFL
                     FVSTMRMGLYLLPQSLGGDQPMTHNVDEINIVTNGDATLVVGEEKIPVKMGSIVWVKE
                     GNPHSFTNPSEDFDVLIAFHIKP"
     gene            complement(549..1019)
                     /locus_tag="VGA21_05280"
     CDS             complement(549..1019)
                     /locus_tag="VGA21_05280"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX9649951.1"
                     /translation="MKIKISVIVLTLLIIAVQSCGQQNMGDIYVKFTEAYQNKDFALL
                     QSLYEDDAYYLSPGGKILNDKNVFLAPIKAMFDDAAEKGLDLHMTFRIVQRDYYGEAA
                     VDIGYYKLDRMNKEGVQGRSIGKFITVARKQVDGSWRFRADAFSDAPEEGFFGN"
     gene            complement(1009..>2033)
                     /locus_tag="VGA21_05285"
     CDS             complement(1009..>2033)
                     /locus_tag="VGA21_05285"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX9649952.1"
                     /translation="KYGVTTVQDMSPLDQVAIYQELKNSGELTCRINFSPSRLSDLDM
                     MVEKGWVINWAGEDGPAAAGDDWISFGTLKTHIDGIMGARTARFYEPYADNGIETPVW
                     RGGWREFSRDLQNFKELILKADNLGIQLRIHAIGDEANSILLDILDTMDMVNGKRDRR
                     FRLVHAQVISSRDFSRFAGRNIIAEVQPYHVTDDMRWMEERIGNERCKGAYAFKTLEE
                     NGCILSFGSDWPGTNASYYPINPLYGIYAAVTRQTLQGKPDEGWFPEQKISLEKALKA
                     YTFGSAFSAFEERKKGTLEKGKLADLIVLDEDLFVTEPEKWLNNSVLYTMVGGKFVFK
                     KSEYEN"
BASE COUNT          571 a          471 c          380 g          611 t
ORIGIN      
        1 tacgaattaa agtctaaagt aattgataat cagccagttc acgatgaaga tgaagtttat
       61 tttattgtgt ccggaaaagc aaaactagta gccggcgaca gcacttttga tgtctccgcc
      121 gggagtattg cctttgtaag agctggtgtt tcgcacaagt ttgttgaaat tgaagaagcg
      181 ttctccgcgc ttgttatatt caccaaaaca ccaacaaaaa caggcgatgc tgacgcgttg
      241 gcttttgagc ttgataagtt aaggcaacca agaaacccgg aagagaacgt ctggaaccag
      301 tttctttttg tttcaaccat gcgaatgggg ttgtacctac ttcctcaatc tctgggtggc
      361 gaccagccaa tgactcataa tgttgatgaa attaatatcg tgacaaatgg tgatgcgacg
      421 ctggtggtag gggaagaaaa aattccggta aaaatgggat caattgtttg ggtgaaggaa
      481 ggcaaccctc attcctttac aaatccaagt gaagattttg atgtcctgat tgcctttcat
      541 atcaaaccct agttaccgaa aaacccttct tccggggcat ccgaaaatgc atccgcccta
      601 aacctccatg aaccatccac ttgtttgcgt gcaaccgtaa taaatttacc aatcgatctt
      661 ccctggacac cctctttatt catcctgtct aatttatagt agcctatatc gactgcagct
      721 tccccatagt agtcacgctg aacaattcta aaagtcatat ggagatctaa acccttttcc
      781 gcagcatcat caaacatagc tttgatgggc gccagaaata cgtttttatc gtttaatatt
      841 ttgccgccag ggcttaaata ataagcatcg tcttcatata aagattgcag taatgcaaaa
      901 tctttatttt gataagcttc tgtaaacttc acataaatat cacccatgtt ttgctgaccg
      961 caagattgaa ccgcgatgat gagtagtgtt aatacgatta ctgaaatttt aattttcata
     1021 ttcacttttt ttgaagacaa atttaccacc aaccattgta tataatacag aattattcaa
     1081 ccatttttct ggttctgtga caaacaagtc ctcatccagc actattaaat cagcaagttt
     1141 ccctttttcg agagtccctt ttttcctttc ttcaaatgca gagaatgccg agccaaaggt
     1201 ataggctttt aaggcttttt ccagggagat tttttgctcc ggaaaccatc cttcatcagg
     1261 cttgccttgt aaagtctgcc gggtcaccgc ggcataaata ccgtagagtg gattaatagg
     1321 gtaatacgat gcatttgttc ctggccaatc cgatccaaaa gaaaggatac atccgttctc
     1381 ttccaatgtt ttgaatgcat aagcgccttt gcacctttca ttgccaattc tctcctccat
     1441 ccatcgcata tcatctgtaa cgtggtaggg ctgaacttcc gcaatgatat ttcttccggc
     1501 aaatcgggag aaatcccggg aacttatcac ttgtgcatgc acaaggcgaa atcttcgatc
     1561 ccttttgcca ttcaccatat ccattgtgtc aagaatatcg agtaatatag aatttgcttc
     1621 atctccaatt gcatgaatcc gcaactggat acccaaatta tccgctttca ggataagttc
     1681 cttaaaattt tgtaaatccc gggagaactc tctccagccc cctctccaga cgggagtttc
     1741 aattccgtta tccgcgtagg gttcataaaa ccgtgcggtt ctcgcgccca taattccatc
     1801 gatatgagtt ttaagcgttc cgaaagaaat ccagtcatct cccgcggcag ccggaccatc
     1861 ttctcccgcc cagttgatta cccatccttt ttcaaccatc atatccagat ctgataatct
     1921 ggatggagaa aaatttatcc ggcaggttaa ttctccggaa ttttttaatt cctgataaat
     1981 tgccacctga tcaagcggtg acatatcctg aacagtggtg actccatatt ttc
//
LOCUS       DASWHX010000076        23260 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_426363, whole genome shotgun sequence.
ACCESSION   DASWHX010000076 DASWHX010000000
VERSION     DASWHX010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 23260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23260
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_426363"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..692)
                     /locus_tag="VGA21_05290"
     CDS             complement(<1..692)
                     /locus_tag="VGA21_05290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649953.1"
                     /translation="MIGSQYFRDFLTLISLAVTITFISCQPDEEKIIDDPSIQLSFSE
                     DTILFDTVFTTIGSITKRFKIYNDRGNAVRVRSIYLGQGINSPYTITVNGLEGKSFDE
                     QIIFGKDSMLILVKVLIDPSTLNMPFIVEDSVVFETAGYPQNVKLLAWGQNANFLNDS
                     IIACNTTWNKDLPYVILNSALVDVNCTLTIESGSAIYFHNNSSLFVAGTLSVEGSNDQ
                     RILFTNDRLDEP"
     gene            complement(694..1764)
                     /gene="prfA"
                     /locus_tag="VGA21_05295"
     CDS             complement(694..1764)
                     /gene="prfA"
                     /locus_tag="VGA21_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018472686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEX9649954.1"
                     /translation="MLDKLQQIKNRFEEISQKMAEPNFMADMTNYKKLSKEYKELKKI
                     VEEYEAYKTILANIKSAKEVLEKEKDQEFREMAKEELDIYYDRQEESEARLKQMLIPT
                     DPNDSKDAILEIRAGTGGDEAAIFAGDIFRMYQRFAEKKGWKFNIIDYAEGPSGGYKE
                     IISTISGEDVYAMMKYESGVHRVQRVPQTETQGRVHTSAATVAVLPEMDDVEVVVNMN
                     DIRKDTFCSSGPGGQSVNTTYSAIRLTHIPTGMVVQCQDEKSQIKNFEKALKVLRSRL
                     YDIEVAKHNEEVGAQRKSMVGRGDRSDKIRTYNYPQGRVTDHRIGYSKHNLPIIMDGE
                     IDDFIEQLRIADNAERLKTGEK"
     gene            complement(1847..2272)
                     /locus_tag="VGA21_05300"
     CDS             complement(1847..2272)
                     /locus_tag="VGA21_05300"
                     /inference="COORDINATES: protein motif:HMM:NF039391.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6265 family protein"
                     /protein_id="HEX9649955.1"
                     /translation="MAGKIRTINNPVKEDQTSFDWLIGLWENAKTKNSFEEWKKGDEN
                     SLVLGQGFRTIKEDRIISEYIQIKKDGDRFIYEARLPHLGDHVPFQLIQINEGGFVCE
                     NPDFDFPKRISYELEGYDTLMVHLFGARKKVVFKFKKSK"
     gene            2948..4318
                     /locus_tag="VGA21_05305"
     CDS             2948..4318
                     /locus_tag="VGA21_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009577511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX9649956.1"
                     /translation="MEKIEGKILIVDDDTGILQTAKVVLRQNFTTVQMEQNPAQIPYL
                     MNTHHFDVVLLDMNFSPGIPEGKEGLQWLEKIKALDQNCQVIMVTAYGEINLAVKAMK
                     YGATDFVIKPWENEKLLATVIAAFKYRKSAQEVLDLKSRQREFLHLLGQQEEIIWGES
                     EGMASIFKTIQKVSATEASVLILGENGTGKEVLAKTIHQLSPRHEQPFIKVDLGAISS
                     GLFESELFGHKKGAFTDAHQDRTGRIELASGGTLFLDEIGNITPEFQIKLLSVLQNRV
                     IIPVGSSMEIPINVRVISASNADINTMIQSGLFREDLLYRLKTVEITLPPLRERKEDI
                     PAFADHFIKVYSAKYRKPTMSLAGDTIQYLQAHSWAGNIRELQHAIERAVIMSDSAVL
                     KKKDFDLQITKVKSDSADSLKLDDVERQTIIDAINKFGGNLSKASLELGLGRTTLYRK
                     IQKYGL"
     gene            4308..5666
                     /locus_tag="VGA21_05310"
     CDS             4308..5666
                     /locus_tag="VGA21_05310"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX9649957.1"
                     /translation="MVFKNLRFNLILRVIILVALLMVLSFVIINTSWFFTPIVLTILI
                     VISVFELFHFMDKTNRELSNFFMSIRQAEFTTIFKGKDRGKSYRELFESLEQLMDEFH
                     KLNLEKESQYQYLQALNENIGVAIISFRENGEIQFMNSAAKLLLRKPRIDKIEDIRSI
                     DKKLFDKILNMQSGDKELVPMVLNNEWVQLSVQSRSFRLQDETFKLIILQNLHHELEE
                     KEVEAWQKLINVLTHEIMNSVTPIVSLVKAVNSMLASNSSIQKLLSEMDADERSDFED
                     SLKTIEERSRGLLKFVNAYREYSTTPDFHPADFDFLNMVQRVYSLMKPELDQNSIRLN
                     LDFGSLKKLNAYGDEQLIEQVIINLIKNAIHALYDSKEPLIELRVKRLGDRKVHLTVK
                     DNGPGISHEVMDRIFVPFYTTRKGGTGIGLSLSRQIMKLHNGKIYADTHPDHGASFSI
                     EF"
     gene            5726..6937
                     /locus_tag="VGA21_05315"
     CDS             5726..6937
                     /locus_tag="VGA21_05315"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020603954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEX9649958.1"
                     /translation="MPDIYKSSIIGAVKAARMDFDPRKKNFDPTFIDFGPVKFYLARH
                     FGFCYGVENAIEISYRALEENQGKNVYLLSQMIHNPEVNNDLEQRGIRFILETDGTPL
                     MDWNEITPEDIVIIPAFGTTLDWESKLSGKGIQIQKYNTTCPFVEKVWNRSYKLAQEN
                     FTIVIHGKHYHEETRATFSRSSHKGKSIIVKDLSEAVALTDFIKGSISREDFMFLFEG
                     KMSQGFDPESDLQRIGVVNQTTMLASETQHIADLIKDALAGTYGVENLSFHYADTRDT
                     LCYATNDNQDATKMLLKTDSDLAIVVGGYNSSNTSHLVELLEVKFPTFFIKSPSEIHG
                     SNRITRYDINKMRSVESEAFLPDKTPVSIAITSGASCPDTLLDAVLLRILQEFNETVP
                     VAEAVSKALLK"
     gene            7412..7813
                     /locus_tag="VGA21_05320"
     CDS             7412..7813
                     /locus_tag="VGA21_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009054639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX9649959.1"
                     /translation="MNITKIKETCLYSDDLEQTKFFYTKYLGLKVINYLEGKHLFLRA
                     GSSVLLFFNPEDSRLKETPPPHFGEGKMHVAFEVSKEDYEKCRLEIENAGIQITHEEI
                     WPGNLRSFYFNDPNGHVLEIVPPGLWEINQK"
     gene            complement(7804..9006)
                     /locus_tag="VGA21_05325"
     CDS             complement(7804..9006)
                     /locus_tag="VGA21_05325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649960.1"
                     /translation="MKQIFTFIKIAGFLFLIGLGISSSAQDTTLMNKDAIYGRPFLAQ
                     MQLGRTTTAVGGYLEANTNYFVSDGIEDGFSMEFRRFNIFLSSQLLPGVRFISELEFE
                     HGTEEINLETALIDFEINPSLIFRGGILLPPVGYFNQNHDSPKWEFVDRPLVSTTIIP
                     STLSEVGFGFHGRFFPFNSQLSYEVYLVNGLGAEIIANEENRTSLRMGKTGILGEDGN
                     RSPALTGRIGWQFRQRGEIGLSYYGGIYNNYKADGLTIDIKRYLNLAALDYNFSFRGL
                     SVLGEAARVWVDIPVSLGQQFGEKQWGFHTDIIYKVIQGRLFHWEGVSLNTALRVEHL
                     DYNEGSFVETGSNIYDHLTALVPGLSLRFSPNTLIRANYRYHWEKDILGNDTVKTAGF
                     QVGFASYF"
     gene            complement(8981..9403)
                     /locus_tag="VGA21_05330"
     CDS             complement(8981..9403)
                     /locus_tag="VGA21_05330"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEX9649961.1"
                     /translation="MKRDVTRRKFVKKTGMATAAILGIGLIPVSCAPFVYGVHYVEAD
                     RIRIPKKEFGRSRFLVIQPQGLKAPVLISKIEEGEYSAVLMECTHKQCTITPAGQILV
                     CPCHGSEYDLRGKVIKSPAVENLITFKVNEDETNIYIY"
     gene            complement(9400..10614)
                     /locus_tag="VGA21_05335"
     CDS             complement(9400..10614)
                     /locus_tag="VGA21_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020531087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HEX9649962.1"
                     /translation="MWKRRWFLALWLLTIGCNTFLPEIPESNSLLEGELAGLTETQNA
                     IFLRGDEAFNEVFTPAKGLGPVFVANSCATCHPGDGKGHPANALTRFGRMNGLQFDPM
                     VEFGGPQLQHRAIPGYVGETVPPEATGVTILVAPANTGLGFIEAITDLQILNWADPDD
                     LDGDGISGRPNWVVPPDFFIPGPDQVSQNGLYIGRFGKKAAALNLTQQTVNAYKQDIG
                     ITSDFDTEDIFNPALGYQTVDNVPDPEVSSATVLDVVFYLRTLKAPERRNTESSEILE
                     GENLFADIGCESCHRANITTGPSEISQLAYKLINPYTDLLLHDMGPGLDDGFTEGSAT
                     TSEWRTPPLWGLGLAGSAQGGKMFLMHDGRARSIEEAILLHGGEAEKSKNNFSELTAE
                     SRDKIIAFLRSL"
     gene            complement(10730..10939)
                     /locus_tag="VGA21_05340"
     CDS             complement(10730..10939)
                     /locus_tag="VGA21_05340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649963.1"
                     /translation="MELEKIKPTTFRGIDFIQIIEIPDDQRQDFISWLTQDQIITVAT
                     NKKVYSRCVQYSLYNEWYNRIKNPH"
     gene            11010..12158
                     /locus_tag="VGA21_05345"
     CDS             11010..12158
                     /locus_tag="VGA21_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009580551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX9649964.1"
                     /translation="MKISQSLKSEFLIPDDISYLNCAYMSPLSKATVAAGKSAVELKA
                     HPYKIKPDDFFETVELIRTEFAKLIYGSAEDVAVIPSVSYGIAMLAKNIELSPGDEII
                     VIEEQFPSNIYSWIRLAKNNGAELTVIKSPETNEKRGMAWNEKILSAISPKTKVVTMG
                     IVHWSDGTLFNLSAIRKKTAKCGSLLLLDGTQAIGALPFNVNEIKPDALIVAGYKWLM
                     CPYATGFAWLGEYFKDGIPLEESWINRKGSSNFAGLVKYQDEFASGARRFDMGEKSNF
                     ALNPMVLTALRQVNEWGPENIQIYVSSISEQPVQELISRGFWVEDKEFRGSHLFGVRV
                     PPGTDLELIRQKCETQNVFVSIRGSAIRVAPHIYNDAHDFEKLIQSLS"
     gene            12251..12475
                     /locus_tag="VGA21_05350"
     CDS             12251..12475
                     /locus_tag="VGA21_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009582339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649965.1"
                     /translation="MLSYTDEQYERARRYVEDMKKHRQRIYWKGKESMSDEELIYSYI
                     AHKILSGFYNHYDPSIAARQILEMKNHTTA"
     gene            complement(12500..15721)
                     /locus_tag="VGA21_05355"
     CDS             complement(12500..15721)
                     /locus_tag="VGA21_05355"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HEX9649966.1"
                     /translation="MKHLKILLCAMIIPFSLSAQEIYREDFNANAQGWYVGTQADYNA
                     AITGGTYVLENKTTSGRFFFNNAEFNENADWTIESNITQLAGITDHGYGIAWGAKDYL
                     NQYCFIATSGGSYMIYSYKDGQYSPIQSWSDAVQAINPPGFVNKFTIKKTGNKLAFFI
                     NDQMVFTSEYEKFLGNKIGVILNDRMKISVEDIKVSIHEQISYDLASLGTIFSDEFND
                     NSKGWLTTSTSAVAANITNGVYQLNHKRTEGQYFFTRELPLDSNEEFEITAGLRQQGG
                     VDNYGYGIAWGGQDDANTYVFYITSSEYYRVYKFQNGSLTDLVQWTTAPGIIKPLGST
                     NQFKIRKSGKTYQFFLNGTLLTSIPAQPLFGNKAGFVLTNSMTVEVERFAISQSGSQS
                     VTQQETKPLETVTTAGGGIFFDSFNNNQNLWSEEASSIHSSRIYSGNYTIEHKGQEGN
                     YFFWKDLSLIGSDTWQIEVTMKQVNGEEYGYGLVWGAADRDNLLGYYITISGYARIWR
                     IQNGQFTVLLDWAQSGSIGGMNTSNNLKIQKQNGALTFFVNNLQVHRMADQPLFGNKL
                     GFVLTSRMTVEVEQLQVEGLPVAEAITQKTVIQKPIIAQTRQAEPEEPPAIRWQIPDQ
                     PVTTTQNRDYSVSLELNSKSPLQNVTLLLNNSVIPFPEAQALSGKSGIITLNGNIKLL
                     PGNNDLKIISANRAGSATSQSRSIIYNQPSPPLITWLIPSQNFSTTSSGKMEIKAGVN
                     STSPVTNIQVLINDIPLADANRGFTVQKQENVIAGYDQVIQKNIELRQGDNLVKLIVE
                     SQNGSITTEVRTITFNSAFASGADMKEEIVGGRKDYAVLFATSNYDNWGDLINPVIDA
                     RAVARELENNYGFEVELVENPTTGQMMTTLKKYANKSYLDKDQLFIFFAGHGTFDETF
                     GEGYVVGANSLLNDEAKTSYLSHSTLRTVINNIPSQHTLVVMDVCFGGTFDQLTARAS
                     HRGDNGVYSELSNTEFIQRKLQYKTRRYLTSGGKDYVPDGDPGKHSPFARKLLEALRS
                     FGGGDSILTFGEVITFMERVVPQPRTAGFGDDEPGSEFLFIAK"
     gene            complement(15875..18412)
                     /locus_tag="VGA21_05360"
     CDS             complement(15875..18412)
                     /locus_tag="VGA21_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009580507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HEX9649967.1"
                     /translation="MKKNLLLLLSLFGPFVAFSQNLRSPDQFLGYELGTKFTRHHRIV
                     EYFNYVADSRPNVKIEQYGETNEGRPLIVAILSNQENFNNLEQIRQDNLKRTGLVSGS
                     PEGSKISIVWLSYNVHGNESSSSEATMKTLYDLANPQNPTTQAWLKNTVVIIDPCINP
                     DGRDRYANFYNQYGNATPNPNLDSKEHNEPWPGGRANHYLFDLNRDWAWQSQVESRGR
                     IKLYNQWMPHVHVDFHEQGLNNPYYFAPAVEPYHEVITGWQRQFQDMLGRNHARYFDK
                     NGWLYFTKEVFDLLYPSYGDTYPTYNGAIGMTYEKGGSGRAGLAARMENDDTVKLADR
                     VEHHYTTGISTVETTSNNAEKVVDEFTKYFDNSRNNPAGQYKSFVVKATNNADKLTAL
                     KDWLDLMGLSHGVAGTRRTAKGFNYHTKRDENFSLEAADLVISMYQPRSNMVKVLFEP
                     DTRLSDSVTYDITAWSIPYVYDLDAFAVAERINPASAPQSPAFTPISGEDKPYAYIGK
                     YQSFKDLQWAAHIMKKGVKVRMADSPFALNGVSYERGTLVVLRWDNEHNKSFDAIIQE
                     SSSRFKKEIIKAATGYVDTGRDFGSSHVGFVKTPNVAVVAGQQVSSLSFGEIWYYFEQ
                     QLEYPVTILDTDYLKNVDLANYDVLIVPNGFYRLFDDSYLGKLRDWVRGGGRLILIER
                     ALNSFVDKEGFSLSKYVKDEDKKKAEEADKKDEENRILARYEDQEREGISYSNPGSIF
                     KIEVDNTHPLAFGYSSRYYALKGTESRFAFLKEGWNVGVIKNSAESRMAGFVGAKVLP
                     DLENTLSFGVEGMGRGQVVYFVDNPLFRAFWYNAKMLFGNAIFFAGQ"
     gene            complement(18489..18812)
                     /locus_tag="VGA21_05365"
     CDS             complement(18489..18812)
                     /locus_tag="VGA21_05365"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HEX9649968.1"
                     /translation="MRWINIFSSIEEARNVIPSGSSKLVIIGETKICLSNFGNAFKAS
                     ENSCPHLGEQLHKGMINYLGEIICPWHNYRFDLSTGEESSGRCRDLLLYQTKTTEEGL
                     FVAIP"
     gene            complement(18815..19396)
                     /locus_tag="VGA21_05370"
     CDS             complement(18815..19396)
                     /locus_tag="VGA21_05370"
                     /inference="COORDINATES: protein motif:HMM:NF040005.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6134 family protein"
                     /protein_id="HEX9649969.1"
                     /translation="MKKLIVGFMFLSIPLFSYSQKLEYAIVWKGDSIGYILIKRALTD
                     STDYHNVRSVSRFRVIFSFTTGYSGETFFKNDQLIKSSLINTMNDDVDGSALVEWLND
                     EYLMIIDGKRTTWNEPVKSTVGLLYFQEPKDLNKIFSERHGRYLRIEHKGNGSYVLTK
                     PDDRINTYTYKNGRCVKVEINNLLTNFSFHLIE"
     gene            19481..20794
                     /locus_tag="VGA21_05375"
     CDS             19481..20794
                     /locus_tag="VGA21_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013452875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX9649970.1"
                     /translation="MKRIIPVFVLTAFFGFSLQAQNEKLKSQIDKKADELETKVIEWR
                     RDIHQNPELSNREFKTSAKVVEHLKSLGIEVQSEVAHTGVVGILKGGRPGPVVALRAD
                     MDGLPVIERADIPFASRVKSTYNGQEVGVMHACGHDTHVAILMGVAEILAGMKNEIPG
                     TVKFIFQPAEEGAPLGEEGGAELMVKEGVLKNPDVQAIFGLHISSGSEINTISYKPGA
                     SQASANSYSIVVKGKQTHGASPWTGVDPIVVSAQIINSIQTIVSRKTNLIRQPAVVTV
                     GSIHGGVRNNIIPEEVKMEGTIRALDNEMRDQIFEDLKTMATKVAESQGATAVVTINH
                     GVPITYNDPSLTGKMLPTLLQAAGKENVHLRPAIMGAEDFSYFQEQIPGFYFGLGGMT
                     PGNATPFPHHTPDFFVDEGGMKLGVRALSYLVFDYLSSTKIDEKK"
     gene            complement(20908..>23260)
                     /locus_tag="VGA21_05380"
     CDS             complement(20908..>23260)
                     /locus_tag="VGA21_05380"
                     /inference="COORDINATES: protein motif:HMM:NF027637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HEX9649971.1"
                     /translation="GMIKHDGYFVFYLEEKTHKIFLEIDKLDTEFLYINSLPAGIGSN
                     DIGLDRGQLGGDRIVKFVHYGPKVLMVQPNYDYRAISQNELEKKAVEQAFAQSVLWGF
                     KVEAEEESKVLIELTDFIMQDTHNVVGRLRSSRQGNYKLDESRSALYPERIKNFPQNT
                     EFEVTLTFTGTPEGGWIRSVTPSPDAITVRQHHSFVQLPDDDYEPRDSDPRAGYFGMS
                     FYDYATPISENIQKRFIARHRLKKKDPTAAMSEPVEPIIYYLDPGVPEPVRSALLDGA
                     NWWNQAFEAAGYRNAFRVEMLPADADPMDVRYNLIQWIHRSTRGWSYGASVSDPRTGE
                     IIKGQVSLGSLRVRQDFLIAQGLIIPYEEGKPASPEMEKLALARLRQLSAHEVGHTLG
                     LAHNYIASYNNRASVMDYPHPLAEINSEGKIVFTNAYDDKIGAWDKVAIRYGYSDFPD
                     GTDVKKALNDILMESARTGLRFISDQDARPQGSAHPYAHLWDNGNDASVELNRLMDVR
                     KKALSQLSENNIKMGEPLFKLEAVMVPIYLLHRYQIEAAAKVVGGVHYDYKIRGDNLA
                     YPTFVSGDDQRAALQALLRTLASEELALPDNLLKLLPPPPIAYGRGREMFTPRTGLAF
                     DYLAPAETAAELTIQLLLHPDRTSRLVEISAIDPSNLFLGETFDAILNATWKKARTPG
                     YKGEVERTVNYVVLQAMFNCVANGDQIPQAQAIASQKLDELDAWLNTQVNRTSNADEK
                     AHLKFAIQKIAQFRKDPEKVIVIEYLRSPDGPPIGMFENWCSN"
BASE COUNT         6688 a         4935 c         4652 g         6985 t
ORIGIN      
        1 taaggttcat ccagccggtc attggtaaaa agaatccgct ggtcattact gccttcaaca
       61 gataatgtac cggctacaaa gagcgaagaa ttattatgaa aataaattgc gctgccgctt
      121 tctatcgtca atgtgcagtt aacatctacc agggcggagt ttagtataac ataaggaagg
      181 tctttattcc aggtcgtatt gcatgctatt atggaatcat tcaagaagtt agcattttgt
      241 ccccatgcca gtagctttac attttgcgga taccccgccg tttcgaaaac aacggaatct
      301 tccactataa aaggcatatt aagcgttgat gggtcaatta gtacttttac caggataagc
      361 atgctgtctt tcccaaaaat aatttgctcg tcaaacgatt tcccttccaa tccgttgacg
      421 gttattgtgt aaggagaatt aataccctgc ccaagataaa tggatcttac cctgacagca
      481 tttccgcgat cgttatagat tttgaaccgt ttggtaatac tgccaatggt agtaaagacc
      541 gtatcaaaca aaatggtgtc ttcagaaaat gaaagttgaa tggatggatc atcaattatc
      601 ttttcctcat ccggctggca gcttataaaa gtaatggtaa cagccagcga aatgagagtc
      661 agaaaatctc taaaatattg acttccaatc aaattatttt tctccggttt tcagcctttc
      721 ggcgttatca gctattcgca gctgttcaat aaagtcatct atttcaccat ccataataat
      781 gggcaggtta tgctttgaat acccaatcct gtgatcggtt acccttcctt gcggataatt
      841 atacgttcgg attttgtctg atctgtctcc acggcctacc atggattttc gttgagcccc
      901 aacttcttca ttatgtttag ccacctctat atcatataaa cgtgagcgaa gcacttttaa
      961 tgctttttca aagtttttta tttgagactt ttcatcctgg cattgcacca ccatacctgt
     1021 tggaatgtgt gttagacgga tagctgaata ggtagtgttc actgattgtc caccggggcc
     1081 cgaagaacaa aaagtatctt tacgaatatc attcatgttc accactacct ccacatcatc
     1141 catttctggt aaaactgcca cagtagctgc ggaggtatga actcttccct gggtttctgt
     1201 ttgcggaacc cgctgaactc tgtgcacgcc ggactcatat ttcatcatcg cataaacatc
     1261 ttctcccgaa atggtactga tgatttcttt gtatccgccg gaagggcctt cggcgtaatc
     1321 aattatattg aatttccaac ctttcttttc agcgaaccgc tgatacatcc tgaatatatc
     1381 ccctgcgaag attgctgctt catcccctcc ggttcctgcc ctgatttcaa gaatagcgtc
     1441 tttgctgtca ttcggatcgg ttggaataag catttgcttt agtctggctt cactctcttc
     1501 ctgtctatca tagtaaatat ccagttcctc ctttgccatc tcccggaatt cttgatcctt
     1561 ttctttttcc aacacttcct tagctgattt tatgttggct aaaattgttt tataggcttc
     1621 gtattcttcg acaattttct tcagttcctt gtactccttg ctaagcttct tataattggt
     1681 catatccgcc atgaagtttg gttcagccat tttttgactg atttcttcaa atcgattctt
     1741 tatttgttga agtttgtcaa gcatagtagt ttaactaaac aggacgcaaa attaactaat
     1801 tttaaaccac cggaattgga atgcgtaaat tattctttac taagtattat tttgattttt
     1861 tgaatttgaa aaccactttc tttcgtgctc cgaacaaatg taccatcagg gtatcatacc
     1921 cctcaagttc ataactaatt cttttaggaa aatcgaaatc cgggttttca caaacaaatc
     1981 cgccttcatt tatttgaatc aattggaagg gaacatgatc acccaaatga ggaagtctgg
     2041 cttcataaat aaaccgatct ccatctttct tgatttgtat atattcactg attattctgt
     2101 cttcctttat ggtgcggaaa ccctgaccca aaaccaaaga gttttcatcg ccctttttcc
     2161 attcttcaaa agaatttttt gtctttgcat tttcccaaag accaataagc caatcaaaag
     2221 atgtttggtc ctccttcaca ggattattta tagttctgat cttacctgcc aatctaagat
     2281 gggtttatag ctgggcttag tgctatgaaa aagcgtagca atatatatta aaatatactt
     2341 cctaattagg aattgaacaa taattaaacc agctgacttt ctaatattcc ccaaatctaa
     2401 atgttttcac cccttattta ttgaagataa aaggtataaa tagcgtagga cggataaata
     2461 ttcaacataa ttaagaaaat tcggactctg gaaattggga ttttaaattt gtgatataac
     2521 tggtggctaa aataaatgta aggagtagaa agccccaata ctaacagcac cattgtgaat
     2581 tgaattatcc attggttaac tacaatttca aattcattat ttaagattaa atatccagcc
     2641 agaatgaaac cggtcaggtt tccagaataa ataaccacaa aaaagaattt tatcgggtca
     2701 tgccatgaac ttttcggttt gataagccgc ttcgaaaagt ttacaaaaaa tgcagatatc
     2761 agaaaaaaca agagtgcgct gaaagtgagt atagctagca tatcccttct caatatccat
     2821 gatatttggc taaatggcaa catttagttc gctatgaatc aatactttaa atttctttca
     2881 aaaatttgtg tttttaaggc ttgtttcatt ttgaaacatt aattttataa agtccattta
     2941 aaagtctatg gagaagattg aaggtaaaat attgattgtt gatgacgaca cggggattct
     3001 tcaaacagca aaagtggttt taaggcagaa tttcacaaca gttcagatgg aacaaaatcc
     3061 agcacaaata ccctacttga tgaatactca tcattttgat gtagtgctgc ttgatatgaa
     3121 cttttctcca ggaattcctg aaggtaaaga aggacttcaa tggcttgaga agatcaaagc
     3181 cttggatcag aattgccagg ttataatggt cactgcgtat ggtgaaatta atttggccgt
     3241 gaaggcaatg aaatatggag ctaccgactt tgttataaaa ccctgggaaa atgaaaaatt
     3301 acttgccacc gttattgctg cattcaaata cagaaaatct gcgcaggagg ttcttgatct
     3361 taaatcaagg caacgtgagt ttcttcacct tcttggacag caagaagaaa taatttgggg
     3421 agaaagtgaa ggcatggctt ctatctttaa gaccattcag aaagtgagcg ctacagaggc
     3481 cagcgtatta atacttggtg aaaatggcac gggcaaagaa gtgctggcca aaacaatcca
     3541 tcaattaagt ccccggcatg aacaaccctt tattaaagtg gatctcggcg caatctcatc
     3601 cgggctgttc gaatcggagt tattcggaca taaaaaaggg gcatttaccg atgcacatca
     3661 agaccgtacc ggtaggattg agttggcttc cggcggaact ttgttccttg atgaaatcgg
     3721 taatataacg cctgaatttc agataaagct tttgtctgtt ttgcagaata gagtaataat
     3781 acctgttgga tcttctatgg aaattccaat caatgtaagg gtaatttccg catcaaacgc
     3841 cgatattaat acgatgattc aatctggttt attccgggaa gatttacttt accggttaaa
     3901 gacggttgaa attaccttac cacccctgcg tgagcgcaag gaagatattc cggctttcgc
     3961 agatcatttt atcaaagttt attcggccaa ataccgcaaa cctacgatgt cattggctgg
     4021 cgatactatt caatatctgc aggcccattc ctgggcgggg aacatcagag aattacagca
     4081 tgcaatagaa agagcggtaa ttatgtcaga ttccgctgtt ctaaagaaaa aggattttga
     4141 cctgcaaatt actaaagtga aaagcgacag tgccgatagt ctgaagcttg acgatgtaga
     4201 aagacagact attatagatg cgatcaacaa atttggcggc aatttaagca aggcttccct
     4261 cgaactgggg ttgggacgaa caactctgta caggaaaatt caaaaatatg gtctttaaga
     4321 atctgagatt taacctgatt ttaagagtca tcatccttgt tgctttgtta atggttttgt
     4381 catttgttat catcaataca agctggttct ttacaccgat tgtactaacc attctcatag
     4441 taatttcagt ttttgaactc ttccacttta tggataaaac aaaccgggag cttagtaact
     4501 ttttcatgag tataaggcag gcagaattta ccacaatctt taaagggaaa gatcgcggca
     4561 aaagttaccg ggagttattt gaatcgctgg aacagcttat ggatgagttt cacaaactca
     4621 accttgaaaa agaaagtcag taccagtatc tacaggcatt aaatgaaaat attggggttg
     4681 caataataag ctttcgtgaa aatggagaga tacagtttat gaattcagcg gcaaaactat
     4741 tgctgcgcaa accacgaatt gacaagatcg aagatatccg gtcaattgat aaaaaacttt
     4801 ttgacaaaat cctgaatatg cagtccggtg acaaagaact cgtgccgatg gttttgaata
     4861 acgagtgggt acaactctca gttcaatcca gatctttcag gcttcaggat gaaactttta
     4921 aactgataat tcttcagaat cttcatcatg aacttgaaga gaaggaggta gaagcctggc
     4981 agaagttaat caatgttctg acccacgaaa taatgaattc agtaactcct attgtatcat
     5041 tggttaaagc cgtaaattct atgttagctt caaattcttc cattcaaaag ctgctgagtg
     5101 aaatggatgc tgacgagcgg tcggattttg aagacagcct caaaacaatt gaagaacgca
     5161 gccgtgggtt attgaaattc gtaaatgcct atcgtgaata ttcaacaaca cctgattttc
     5221 atcccgccga tttcgatttc cttaacatgg tccagcgggt atattcatta atgaaacccg
     5281 aacttgatca aaactccatc cggttgaatc ttgattttgg aagcttgaaa aagttaaacg
     5341 cttacggtga tgaacaatta attgagcagg tgatcatcaa tttgattaag aatgcaattc
     5401 acgcccttta tgatagtaaa gaaccattaa ttgaacttag ggttaagcgc cttggtgatc
     5461 gtaaagttca tctgacagtt aaagataatg gtccgggaat ctcgcatgag gtaatggata
     5521 gaattttcgt tcccttctat actacccgta aaggaggcac cggaattggg ctaagtcttt
     5581 ccaggcaaat catgaaactt cacaacggaa aaatatacgc ggatacgcat cctgatcacg
     5641 gagcttcttt tagtattgaa ttctgaatgc taagagttct tacattacct ttgctataaa
     5701 tcctgaagtt atcagaaagt ttaacatacc agatatctat aaatcttcca taataggagc
     5761 agtgaaggcg gcaagaatgg atttcgaccc gcggaaaaaa aactttgatc ctacctttat
     5821 agactttggt ccggttaaat tttatttggc gcgacatttt ggcttttgtt atggtgttga
     5881 aaacgcgatt gaaatctcat acagagctct tgaagaaaat cagggcaaaa atgtttacct
     5941 gctcagccag atgattcata atcccgaagt taataatgat cttgaacagc gcggcatccg
     6001 gtttattctc gaaacggatg gaaccccctt aatggattgg aatgagataa ctccggagga
     6061 catcgtaata atacctgcat ttggaactac gttggattgg gaatcaaaac tttctggaaa
     6121 aggcattcaa attcaaaaat acaataccac ttgccccttc gttgaaaagg tatggaaccg
     6181 gtcttacaag ctggcccagg aaaatttcac aatagttatt catggtaaac attatcatga
     6241 agagaccagg gcgacatttt caaggagcag ccataaaggc aaatccatta ttgtgaaaga
     6301 tctcagtgag gcggttgctc tgacggattt cataaagggg agtattagcc gagaagattt
     6361 tatgttctta tttgaaggga aaatgtcgca aggttttgat cctgaatcag acctgcaaag
     6421 aatcggtgtc gttaatcaaa caaccatgtt agcatcagag acccagcaca ttgcagattt
     6481 aataaaagat gcattggccg gaacatatgg tgtagaaaac ctgtccttcc attacgccga
     6541 cacaagggac accctttgtt atgcgaccaa tgataaccag gatgcaacta aaatgctact
     6601 caagacagat tcagaccttg ccatagttgt tggagggtat aatagttcaa atacttctca
     6661 tttggtagaa ttgctggaag tcaaattccc tacttttttc attaagtcac cctctgagat
     6721 tcacggttca aatcgaatta cacgttacga tatcaataag atgcgatccg tggaatctga
     6781 agcttttctt ccggataaaa ctcctgtttc catcgctatt acaagtggag catcatgccc
     6841 ggatacattg ttggatgccg tactgcttcg catcttgcaa gagttcaatg aaactgtacc
     6901 cgtggcggag gcagtaagca aggcacttct gaagtaagta gctcatttta aagaccttaa
     6961 gcgaatttag tatgtggata agaggtgcat aaatgtgtgc aaaagttgtt gaaaataagt
     7021 atcatcaaaa gttgacttgc ctgatggtct tatctacctt gcttttacca agtgagtgaa
     7081 aaaggtgcag aaccgattgg ctaaggctct gagcatttga actcacgaaa tttgttgcaa
     7141 aagggttggt aaccgcaagg tgacaatctg aaatggctca gccacaaagt atcaggtttc
     7201 ataggatctg aaacgaaagt tttggttatg agagttgagt gactggggtc agattcagga
     7261 gcgttaggca cttcggtgtt tagcaggaat gatgaagtag ctttactgta acttggcatg
     7321 tgcagcagcc tcggttgttg caggtgagac gggaatctta tcttaattga tatggttccc
     7381 gtttacgttt taagacactc cccttttaag aatgaacatc actaaaatca aagagacgtg
     7441 cttgtattct gacgacctgg aacaaaccaa atttttctat actaaatacc tcggcttaaa
     7501 ggtgattaat tatctggagg gaaaacatct cttcttaaga gccgggtctt ccgtcctgct
     7561 tttctttaac cctgaagatt ccagattaaa agagactccc ccaccacatt ttggcgaagg
     7621 taaaatgcat gttgcctttg aagtgtccaa agaagattat gaaaaatgca ggcttgagat
     7681 tgaaaatgca ggaattcaaa taactcatga agaaatctgg ccaggtaacc taaggtcatt
     7741 ttactttaat gacccgaatg gtcatgtgct ggaaattgtg cctcccgggt tatgggaaat
     7801 aaatcagaag tagctggcaa atcctacctg gaatccggcc gtttttaccg tgtcatttcc
     7861 cagaatatct ttttcccaat gataccggta gtttgccctg attaacgtat tgggagaaaa
     7921 acgcaaactt aaacctggca ctaaagcggt taaatgatca taaatgttgc tcccggtttc
     7981 aacaaaactt ccctcattgt aatccagatg ttcaactctt agggcagtat tcaggctcac
     8041 cccttcccaa tgaaataatc ttccttgtat aaccttgtaa ataatatccg tatgaaagcc
     8101 ccattgcttt tcgccaaact gctgacccaa tgaaaccgga atatcaaccc agactcttgc
     8161 agcctcacca agcacgctta atcctctaaa agaaaaatta taatccaatg ccgcaaggtt
     8221 taaataccgt ttgatgtcaa ttgttaatcc atcagctttg taattattat aaattcctcc
     8281 ataataggac aaacctattt ctcccctttg cctgaattgc cagccgattc tacctgttag
     8341 tgccggactt ctgtttccat cttcccccag gatgcctgtc tttcccatcc tcaaagaagt
     8401 acgattttct tcattggcaa taatttcagc acccagacca tttaccaggt agacttcata
     8461 agaaagctga gaattaaaag gaaaaaatct gccgtgaaag ccaaatccaa cttctgagag
     8521 tgttgatgga atgatggttg tagaaaccaa aggtctgtcc acaaattccc attttggact
     8581 atcatgattc tgattgaagt atccaaccgg tggaagtaaa attccgcccc tgaaaatcaa
     8641 ggaaggatta atttcaaaat cgatgagtgc tgtttccaga tttatttctt ccgttccatg
     8701 ctcaaattcc agttcggaaa taaatctaac tccgggaagg agctgcgatg ataagaaaat
     8761 attaaatcgt ctgaattcca tggagaatcc atcctcaata ccatcactga caaaataatt
     8821 ggtgtttgcc tcgaggtatc cgccaacagc agttgtagtt ctacccagct gcatttgcgc
     8881 aaggaatgga cgaccataaa ttgcatcctt gttcatcaag gttgtgtctt gagctgatga
     8941 agagatgccc aggccaatca agaataggaa gcctgctatc ttaataaatg taaatatttg
     9001 tttcatcttc attcacttta aaggttatta aattttccac cgccgggctt tttataactt
     9061 tacccctcag gtcgtactcg cttccatggc aagggcagac tagaatttga cctgccggag
     9121 taatagtgca ttgcttgtga gtgcattcca tcagcacggc tgaatactcc ccttcttcaa
     9181 ttttacttat taggaccggt gcttttaatc cctgtggctg aatcaccagg aacctacttc
     9241 tgccaaattc tttcttcggt atcctgattc tgtctgcttc cacataatga acaccataaa
     9301 caaatggcgc acaactaaca ggaataagcc caatccccag aattgcggcc gtagccattc
     9361 ctgttttttt gacaaacttc ctccgggtta catccctttt cataaggagc gtagaaatgc
     9421 gataatctta tccctggatt ctgcggtcaa ttcagaaaag ttattcttac ttttttccgc
     9481 ttcaccaccg tgcaataaaa ttgcttcttc aatgctgcgg gctctgccgt catgcattag
     9541 aaacatcttg cctccctgtg cgctgccggc aagtcctaaa ccccaaagcg gaggtgtccg
     9601 ccattctgat gttgttgcag aaccttcagt aaagccatcg tctaaacctg gacccatgtc
     9661 atgcagcagg agatctgtat atggattgat taatttatac gcgagttggc ttatttctga
     9721 aggtccggta gtgatattgg ctctatgaca gctttcgcag ccaatgtctg caaaaagatt
     9781 ttcgccctcc aggatttcag aagactctgt atttcttctt tcaggagctt taagagttct
     9841 caaatagaat actacatcaa gaactgttgc cgatgacact tccggatccg gaacattatc
     9901 aacggtttga tatcctaaag ccggattgaa aatatcctcc gtatcaaaat cagaagttat
     9961 acctatatcc tgtttatagg cattgactgt ttgttgcgta agattcaagg cggctgcttt
    10021 cttcccaaat cttcctatgt acaagccatt ctgcgatact tggtcagggc ccggtataaa
    10081 aaaatcgggt ggaactaccc aatttggcct gcctgaaatt ccatccccgt ctaaatcatc
    10141 aggatcagcc cagtttaaga tttgaaggtc agttatagct tcgataaatc ctaatccggt
    10201 attagccgga gccaccaata ttgtaacacc ggttgcctca ggaggaacgg tttcacctac
    10261 atatcctgga attgcccgat gctgaagctg tggaccgcca aactcaacca tcggatcaaa
    10321 ttgcaatcca ttcattctcc caaacctggt aagtgcattc gcagggtgac ccttcccatc
    10381 accaggatga caagtagcac aagaattcgc cacgaaaaca gggcctaacc cttttgctgg
    10441 agtaaaaacc tcattgaagg cttcatcccc gcgcagaaaa attgcgtttt gagtctcagt
    10501 aagaccagca agctctccct ccagcaatga attgctttcg ggtatctcag gcaggaaggt
    10561 attgcaacct attgttagca accatagagc caaaaaccat cttctcttcc acatatcggg
    10621 gattttttcg tgaagttaca aaaaatatta ttatccccaa aaatatattt ttaggcttgt
    10681 ctaaattaat gaaagttaat gagtaatgta ttagtttgtt tatggaagtc tagtgaggat
    10741 ttttgatgcg gttataccat tcgttataca atgaatattg cacgcacctg gagtaaactt
    10801 tcttgtttgt agcgacggta attatttggt cctgagtaag ccaggaaatg aagtcttgcc
    10861 tctgatcatc agggatttcg attatctgga taaagtcaat tcccctaaat gtggttggtt
    10921 ttattttctc gagttccata ttttaccagc ctgcttatta tcttaagata cattttatac
    10981 ttttaccttc atttttgtaa cctgtttcta tgaaaatttc gcaatccctg aaatcagagt
    11041 ttttgatccc cgacgatatt tcctatctca actgcgctta tatgtcaccc ctttccaaag
    11101 cgaccgttgc tgcaggaaaa agtgcggttg aattaaaagc ccatccttat aaaatcaagc
    11161 cggatgattt ttttgagact gtcgaattaa tccggacgga gtttgcaaaa cttatttatg
    11221 gatctgcgga agatgtagct gtaattccaa gtgtatcata tggaattgcc atgcttgcta
    11281 aaaatattga attatccccg ggggatgaaa ttatcgttat tgaagaacaa tttccgtcaa
    11341 atatatatag ctggattcgc ctggcgaaaa acaacggagc tgaactcacc gtaattaaat
    11401 ctcctgaaac gaatgagaaa aggggtatgg catggaatga aaaaattcta tcagccattt
    11461 ctccaaaaac aaaagtggtt acgatgggga ttgtccactg gtcagatgga actcttttca
    11521 atctttcagc cattcgtaaa aaaactgcta aatgcggatc attactatta ttggatggaa
    11581 ctcaagctat cggtgcactt ccatttaatg taaatgagat aaaacctgac gcgctcattg
    11641 tcgcagggta taaatggctg atgtgcccat acgctacggg ttttgcctgg ctcggcgaat
    11701 atttcaaaga cggcattccg cttgaagaga gttggatcaa ccgtaaaggg agctcaaatt
    11761 ttgcaggcct tgtaaagtat caggatgaat ttgcttccgg tgctcgccgg tttgacatgg
    11821 gagaaaaaag caacttcgcc ttaaacccga tggtactaac cgcattacgg caggtgaatg
    11881 aatggggacc cgaaaacatt cagatttatg tatcctccat ttccgaacaa cccgtacagg
    11941 agctgatctc ccgtgggttt tgggttgaag acaaggagtt tcgtggctct catttatttg
    12001 gcgtccgggt tcctcctggt accgacctgg aattaatcag gcaaaaatgt gaaacgcaga
    12061 atgtttttgt ttccatcagg ggttctgcta tccgggttgc ccctcatatc tataacgatg
    12121 cccatgattt tgagaagtta atccagtcat taagctgatt gttctaaaaa catttgaatt
    12181 ttttttctta ttgaaataaa ttggcagaac ttaagattga tattcgatgc cctgtgttga
    12241 aacaaccttc atgctaagtt atactgatga acaatatgag cgagccagga ggtacgtgga
    12301 agacatgaag aaacaccggc aacgaattta ctggaaaggt aaagagagca tgtctgatga
    12361 ggaacttatc tattcctata tcgcgcataa aatcctgagt ggattttata accattacga
    12421 tccttcaatt gctgctaggc agatacttga aatgaagaat cacaccaccg cctgatgaat
    12481 tttattaaaa atttcctggc tatttcgcta taaaaagaaa ctcactgcca ggctcatcat
    12541 ctccaaaacc cgcagttctt ggttgcggaa cgacccgctc cataaaggtg attacttcac
    12601 caaaggttag gatgctgtct cctcctccaa aacttcgcaa ggcttccagc aatttccttg
    12661 caaatggtga atgtttcccg gggtcgccat ccggaacata atccttgccg cccgaagtta
    12721 aatatcgacg agtcttatat tgtaatttgc gctgaatgaa ttctgtgttg cttaattcag
    12781 aataaacacc gttatcacca cgatggctgg ctctggctgt taattgatca aaagttccac
    12841 cgaaacaaac atccattaca accagggtat gttgactggg aatgttgtta ataactgttc
    12901 ttaaagttga gtgcgacaaa taggatgttt tagcttcatc attcaataat gaattggcgc
    12961 caaccacgta accttcgcca aaggtttcat caaatgttcc atgcccggca aagaaaatga
    13021 aaagctggtc tttatccaga tatgatttgt ttgcatactt cttcagggtt gtcatcattt
    13081 gtcctgtggt tggattttca accaactcca cttcaaatcc gtaattattt tccaattccc
    13141 tggcaacagc cctggcgtca ataaccggat ttattaaatc accccaattg tcataattac
    13201 ttgtggcaaa tagaaccgca taatctttcc ttcctcccac aatttcttcc ttcatgtcag
    13261 cccctgatgc aaaggcgcta ttaaacgtta tggttcttac ttctgtagtt attgaaccat
    13321 tttgactttc cacaattaac tttaccagat tgtccccttg cctcaattca atatttttct
    13381 gaataacctg atcatatcct gcaattacat tctcttgttt ctggacagtg aatcccctgt
    13441 tcgcatccgc caatgggatg tcattaatta gcacctgaat attggttacc ggagaagtag
    13501 aatttactcc ggctttaatc tccattttgc cggaacttgt tgtgctgaaa ttctgactgg
    13561 gaatcagcca ggtgattaat ggtggagaag gctgattata gattatggat ctgctctggg
    13621 aggttgcact tccggctctg ttggctgaaa tgattttcaa atcattatta ccgggaagca
    13681 attttatatt tccattaagt gtgataatac ctgacttgcc tgagagtgct tgagcttcag
    13741 gaaatggaat aacgctgtta ttcagcagta gtgtaacatt ttgtaagggc gacttcgaat
    13801 tcaattccag ggagacagaa taatcccggt tctgtgttgt tgttacaggt tgatcgggga
    13861 tttgccatcg gatagcaggt ggctcttccg gttctgcttg tctggtttgt gcgattattg
    13921 gtttttgaat taccgttttc tgggttattg cctcggctac cggcaagcct tccacctgaa
    13981 gttgctccac ttcaactgtc attctactgg tgagaacgaa tccgagcttg ttaccaaata
    14041 acggctggtc agccatcctg tgaacctgaa ggttgtttac aaagaacgtc agcgcgccat
    14101 tctgcttttg gattttcaga ttgttggatg tattcattcc gccgatactg ccactttgag
    14161 cccagtcaag caagacagtg aattgtccgt tttgaattct ccagattctg gcgtaaccgg
    14221 aaatggtaat atagtaaccc agcaggttat cccggtctgc agcgccccaa accaatccat
    14281 atccgtattc ttcaccgttt acctgtttca tcgtaacctc aatttgccag gtatcactgc
    14341 cgatcaggga aagatctttc caaaaaaagt aattcccttc ctgaccttta tgttcaatag
    14401 tataattacc gctataaatt cttgatgaat gaatcgaaga tgcttcttca gaccacagat
    14461 tttggttgtt gttgaaacta tcgaaaaata ttccacctcc tgccgttgta actgtttcca
    14521 gaggttttgt ttcctgttgc gtcactgact gacttcccga ctgactgatg gcgaaacgct
    14581 ccacttcaac agtcatagaa ttcgtcaaca caaaccctgc tttattgcca aacaatggtt
    14641 gagctggaat agaagtcagt aaggttccat tcaggaaaaa ttgataagtt ttacctgatt
    14701 ttcttatttt gaattgattg gttgatccca aaggttttat tattcccgga gcagttgtcc
    14761 attgtaccaa atcggtaagg gatccatttt gaaatttata aactctgtaa tattcgctgc
    14821 tggtgatata aaacacataa gtgttggcat catcttgtcc accccaggcg attccataac
    14881 cataattatc tacgccaccc tgctgcctca ggccagcagt aatctcaaat tcttcatttg
    14941 aatccagagg caattccctg gtgaaaaaat attgaccttc cgttctttta tgatttagct
    15001 gataaacgcc atttgtaata ttggctgcta cagcacttgt ggaagttgta agccaacctt
    15061 tggagttatc attgaattca tctgaaaaaa tagtgcctag tgatgccagg tcataagaga
    15121 tctgttcatg gatggaaacc ttaatatctt caacggaaat tttcatcctg tcattaagga
    15181 taactccaat tttgttcccc aaaaactttt catattcaga ggtaaatacc atttgatcat
    15241 taatgaaaaa ggctaattta tttcctgtct ttttaatggt aaatttatta acaaatccag
    15301 gcggattaat tgcctgaact gcatctgacc acgattgaat gggactgtat tggccatctt
    15361 tataactgta gatcatgtaa ctgccaccgc tggtagcaat gaaacaatac tggttgagat
    15421 aatcctttgc tccccatgca ataccataac catgatcggt aattcctgct aactgcgtga
    15481 tgttgctttc aatagtccaa tcggcattct cattgaattc cgcgttgtta aagaaaaacc
    15541 ttccgctcgt tgttttattc tcaagtacat acgtgccacc ggtaattgcc gcattataat
    15601 cagcctgagt tcccacatac caaccctgag cgttagcgtt aaaatcttcc cggtatattt
    15661 cctgtgctga taaagagaaa ggaattatca ttgcacagag aagaattttc aggtgtttca
    15721 tcgccttcgg tttagaatag taaaattagc aagtagcgca gagaattaaa aatggcttaa
    15781 atgcttgatt tctacctatt tgtacattga aaaccaaaaa ggtaacctag ttctttgcgg
    15841 taatgtgaag gaaactccgc atgcggcaat taccttactg ccccgcaaag aaaatagcgt
    15901 ttccaaaaag cattttcgca ttataccaga acgccctgaa caaggggttg tcaacaaagt
    15961 atacaacttg tccccttccc ataccttcaa ctccgaaaga aagcgtattt tccagatcgg
    16021 gaagtacttt tgctccaaca aaaccagcca ttctggattc agcagaattc ttaatcaccc
    16081 caacattcca cccttccttt aagaaagcaa atctggattc tgtgcctttc aaggcatagt
    16141 aacggctgga atacccaaat gccagcggat gagtattgtc cacttcaatt ttaaagatgg
    16201 accctgggtt cgaatacgaa attccctctc tttcctgatc ttcatacctg gccagaattc
    16261 tgttctcctc atctttctta tctgcctctt ccgccttttt cttatcttca tcttttacat
    16321 atttgctcaa ggaaaaacct tccttgtcaa caaaagaatt cagtgctctt tcaatcagga
    16381 taagtcttcc tccccctcgt acccaatccc ggagtttacc cagatagcta tcatcaaaaa
    16441 gtctgtagaa accattggga actatcagca catcataatt agctaaatca acatttttga
    16501 gataatcggt gtcaagaatt gttaccggat attcaagttg ttgttcaaag taataccaga
    16561 tttcaccgaa cgaaagcgat gaaacttgct ggcctgctac cacggcaacg tttggagttt
    16621 ttacaaaacc aacatgactt gatccgaaat ccctgccggt atcaacatat cctgtggctg
    16681 cttttatgat ttcttttttg aatctggatg atgattcctg tatgatggca tcaaaggatt
    16741 tattatgctc gttgtcccat cgtaaaacta ctagcgtacc tctttcgtat gatacaccat
    16801 taagcgcaaa gggtgagtct gccattcgta cttttactcc tttcttcata atgtgtgcag
    16861 cccattgcag atctttaaag ctctggtatt tgccgatata tgcatacggt ttatcttcac
    16921 cggaaattgg tgtaaaagcc ggtgactgag gtgcagatgc cggattaatc ctttcagcaa
    16981 ccgcgaaagc atccaaatca taaacgtagg gaatcgacca ggcggtgata tcataagtaa
    17041 ccgagtcgga aagtctggtg tcgggttcaa acaaaacttt aaccatgttc gaccggggtt
    17101 gatacatgga aataaccagg tcagcggctt ccagggaaaa gttttcatcg cgctttgtat
    17161 ggtaattaaa tcctttagcc gttctgcgcg taccggcaac accatgtgaa agtcccatta
    17221 gatcaagcca gtctttcagc gcagtaagtt tgtcggcgtt gttggttgct ttaacaacaa
    17281 atgatttgta ttgacccgcc ggattattcc ggctattgtc aaagtatttt gtgaattcat
    17341 caaccacctt ttccgcatta ttggaggtag tttctactgt agaaatacct gttgtgtaat
    17401 gatgttcaac ccggtcggca agttttacag tatcatcatt ttccatcctt gccgctaaac
    17461 ctgccctccc gctgccgcct ttctcatagg tcatcccgat agctccattg taagttggat
    17521 aggtatctcc atagctggga taaagcagat caaaaacttc ttttgtaaaa tacaaccagc
    17581 cgttcttgtc gaaatatctc gcatgatttc ttcccagcat atcctggaat tggcgctgcc
    17641 agccggttat aacctcatga taaggttcaa cggccggtgc aaagtaatat ggattgttca
    17701 gcccctgctc atggaagtcc acatgaacat gcggcatcca ttgattatat aatttaattc
    17761 tacctcttga ttctacctga ctttgccagg cccagtccct gttcaaatcg aaaaggtaat
    17821 gatttgctct tccccccggc cagggttcat tatgttcttt ggaatccaga ttgggatttg
    17881 gagttgcgtt tccatattgg ttataaaagt ttgcatatct atctctgcca tctggattta
    17941 tgcagggatc aattattaca actgtatttt tgagccaggc ctgggttgta ggattctgtg
    18001 ggtttgccag gtcatacaaa gttttcatag tggcttcaga tgacgaagat tcatttccgt
    18061 gcacattata gcttagccac acgatggata ttttactccc ttccggcgag ccggacacca
    18121 atccggtacg ttttaaatta tcttgccgga tttgttcaag attgttgaaa ttttcctgat
    18181 tactcaggat ggccacaata agaggacgac cctcattggt ttcgccatat tgttcaatct
    18241 tcacatttgg cctggagtcg gccacgtaat taaaatattc tacgattcga tgatgacggg
    18301 taaatttggt tcctaattca tagcccagga attgatccgg agatctaaga ttttgagaaa
    18361 atgcaacaaa aggtccaaac aatgacagga gcaacaacag attctttttc ataatgaata
    18421 atttaagccc ggctaattta cgtattcggt taaagtcagc aaatcattta gttaataagc
    18481 ggtgaaaatc agggaatcgc tacaaaaaga ccttcttcag tggtctttgt ttgataaagt
    18541 aaaaggtccc ggcatcttcc ggaactttct tctccggtag ataaatcaaa ccggtaattg
    18601 tgccatgggc atataatttc gccaagatag ttaatcattc ctttgtgtaa ttgttctccc
    18661 aggtgaggac agctgttctc gctggcttta aaggcattcc cgaagttcga cagacaaatt
    18721 tttgtttcac caatgattac cagctttgag cttccggatg gaatcacatt ccgcgcctct
    18781 tcaattgaac tgaaaatatt tatccatcgc ataactactc tatgagatga aatgaaaaat
    18841 tggtaagcag gttgtttatt tcaaccttaa cacagcgtcc gtttttataa gtataggtat
    18901 ttatacgatc atcaggtttg gtgagtacat aacttccgtt acctttgtgt tcaatcctca
    18961 aatatcttcc gtgcctttca gagaaaattt tatttaagtc cttaggttcc tggaaatata
    19021 aaaggcccac cgtcgatttt acaggttcat tccaggtggt tctcttccca tcgattatca
    19081 ttaaatattc atcatttaac cactcaacca aagctgagcc atccacatca tcattcattg
    19141 tgttaattaa acttgatttt atcaactggt catttttgaa aaatgtctca cctgaatacc
    19201 cggtggtgaa actgaaaatc actctaaacc gggatacgct tcttacgttg tgataatctg
    19261 tggaatctgt gagtgcccgc ttaatcagaa tataccctat ggaatctcct ttccaaacaa
    19321 ttgcgtattc caatttttgg gagtaagaaa ataatggtat ggacaaaaac atgaatccaa
    19381 caatcaactt cttcatatta ccaacttaat aaaatttttg tttaactttt gtccgctttt
    19441 aaaagaaata taattttcaa aatactctaa acaatttatt atgaaaagaa ttatacctgt
    19501 ttttgtactg actgctttct ttggattcag ccttcaggct caaaacgaaa aattaaaatc
    19561 ccagattgac aagaaagctg atgaactgga gactaaagtt attgaatggc gaagagatat
    19621 tcaccaaaat ccagaactat caaatagaga gttcaaaacg tccgctaagg ttgttgaaca
    19681 tttaaagtcg ctgggaattg aagttcaatc agaagttgcc cataccggtg ttgttggtat
    19741 tttgaagggt ggcaggcccg gtccggttgt tgccttacgc gccgatatgg atggcttacc
    19801 tgttattgag cgggcagata ttccctttgc ttccagagta aagagtacct acaatgggca
    19861 ggaagtagga gtaatgcatg cctgtggtca tgatacgcat gtggcaatac taatgggcgt
    19921 ggcagagatt cttgccggga tgaagaatga gattcccgga actgttaaat tcatttttca
    19981 gcctgctgaa gagggagcgc cgctcggtga agagggcggt gctgaattaa tggtaaaaga
    20041 aggtgttttg aaaaacccgg atgtgcaggc catctttggt ttacatattt cttcaggctc
    20101 ggagattaac actatttctt acaaacccgg agcatcccag gcgagtgcca atagttactc
    20161 aattgtggta aagggcaagc aaacacacgg agccagccca tggacaggtg ttgaccctat
    20221 cgtagtttcc gcacagataa tcaacagtat tcaaactatt gtcagcagga aaactaatct
    20281 gattaggcaa ccggcggtag taactgttgg atccattcat ggcggggttc gtaataacat
    20341 aattcctgag gaagtgaaga tggaaggaac tattcgtgct ttggataatg aaatgcgtga
    20401 tcagattttt gaagacttga aaaccatggc caccaaggta gccgaaagtc agggtgcaac
    20461 ggcggttgta actattaacc atggagtgcc cataacttac aacgatcctt cgcttaccgg
    20521 gaagatgttg cctactttgc tgcaagcagc cggaaaagaa aacgtacatc tacgccccgc
    20581 cattatggga gctgaggatt tctcctactt ccaggagcaa attcccggtt tttattttgg
    20641 tctcggtggg atgactcccg ggaacgccac cccttttccc caccataccc cagacttttt
    20701 tgttgatgaa ggcggcatga aactgggggt tagagcgttg tcatatctgg tttttgatta
    20761 cttaagcagc acgaagattg atgagaagaa atagatagtt tgaagagggt tgcgaatgtg
    20821 gtgcgagtgc gagtgttggt tgtaaatgtg agtgtgaggt gagaatggga agacccttca
    20881 actattttga atacctgcat ctcatattca attcgagcac caattttcaa acattccgat
    20941 tgggggacca tccggcgaac gaaggtattc tattactata accttctccg gatcttttct
    21001 gaattgagca attttctgga tagcaaattt taagtgagcc ttttcatccg cgttgctggt
    21061 tctgttgacc tgggtgttta accatgcatc aagctcgtct aacttctgcg aagcaattgc
    21121 ctgggcctgg ggtatttgat caccatttgc aacacaattg aacatggcct gaagaacaac
    21181 gtaattcact gttctttcca cttcaccttt atacccgggg gttctggctt ttttccatgt
    21241 ggcattcaga atggcatcaa aagtttcacc caggaaaaga tttgaaggat caattgcaga
    21301 gatttctacc agtcgtgaag ttcggtcagg atgcagaagc aattggattg tcaattcggc
    21361 agccgtttca gccggagcca ggtaatcaaa tgctaatccg gttcttggag tgaacatctc
    21421 ccttcccctt ccatatgcaa tcggcggtgg aggtaggagt ttcaagagat tgtcaggaag
    21481 tgcaagttcc tccgatgcca gcgtccgtag caaggcttgc aaggccgccc gttgatcgtc
    21541 cccggaaacg aaagtcgggt aagcaagatt atcaccgcgg attttataat catagtgaac
    21601 tccacccacc accttggcag cagcctcaat ctggtaccgg tgcaataaat aaattggaac
    21661 catcacagct tcaagtttga ataaaggttc gcccattttt atgttgtttt cagacaactg
    21721 tgacaacgcc ttctttctaa catccatcaa tctgttcaat tccacggagg catcattgcc
    21781 attatcccaa aggtgtgcat aaggatgcgc gcttccctgc ggcctggcat cctgatcgga
    21841 aataaaccgg agccctgttc gggcggattc catcagaatg tcattcaacg cttttttcac
    21901 gtcagttcca tcaggaaaat ctgaatatcc atatcggatt gccactttgt cccacgctcc
    21961 tattttgtca tcataagcgt tggtgaaaac aattttccct tcggaattaa tctcagccag
    22021 cggatgggga taatccatta ccgaggctct gttgttgtaa ctggcaatgt aattatgcgc
    22081 cagtcccaaa gtatgaccaa cttcatgagc tgacagctgg cgcaatcggg ctaacgcaag
    22141 tttttccatt tccggagatg ccggttttcc ttcttcatag ggaataatca atccctgggc
    22201 gataagaaaa tcctgacgaa ccctgagaga gccaagagat acctggcctt taataatttc
    22261 accggtgcgc gggtcggata ctgaagcacc ataagaccag ccgcgggtgg agcggtgaat
    22321 ccattgaatc aggttatagc gaacatccat cgggtcggcg tcagcaggta gcatttctac
    22381 cctaaatgca tttcgatatc cagctgcttc aaatgcctgg ttccaccaat tcgcaccatc
    22441 caataacgcg gatcgcacag gttcaggcac tcccgggtcc aggtaataga taatgggttc
    22501 aacaggttca ctcattgccg cagtcggatc ttttttcttc agcctgtgtc gtgctataaa
    22561 tcttttttga atattttcag aaatgggtgt tgcataatca taaaatgaca tgccaaaata
    22621 tcccgccctg ggatcagaat ctctgggctc gtaatcatca tccggaagtt gtacaaaaga
    22681 atgatgttga cgaactgtta tggcatccgg ggaaggcgta acactacgta tccagcctcc
    22741 ttccggagtt ccggtaaaag ttaatgttac ttcaaattcc gtattctgtg gaaaattttt
    22801 tatcctctcc ggataaagtg cagaccgcga ctcgtccaat ttgtaatttc cctggcgtga
    22861 actgcgcagc cggcctacaa cattgtgagt atcctgcata atgaaatcag tcagttcgat
    22921 taagactttg ctttcttcct ccgcttccac tttaaaaccc cataaaacag attgggcaaa
    22981 cgcttgttca acagccttct tttccaattc gttttgggag attgccctgt aatcatagtt
    23041 gggttgtacc atcaacactt tgggtccgta gtgaacaaat ttcacaattc tgtcgccacc
    23101 cagttggcca cgatccaatc caatatcatt cgaaccaata ccggcgggga gagaattgat
    23161 atagagaaac tccgtatcca acttatcgat ttccagaaat atcttatggg tcttctcctc
    23221 aagataaaac acaaaataac cgtcatgttt gatcatgccc
//
LOCUS       DASWHX010000077        37601 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_801236, whole genome shotgun sequence.
ACCESSION   DASWHX010000077 DASWHX010000000
VERSION     DASWHX010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 37601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37601
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_801236"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..681)
                     /locus_tag="VGA21_05385"
     CDS             complement(<1..681)
                     /locus_tag="VGA21_05385"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9649972.1"
                     /translation="MELNLQNKKVLITGSSRGIGKAITKAFVGEGSIVGAHYNASKKD
                     AEALITGKGIALQADFSNPGKSSGIIDEFIAFSGGLDVLILNAGIAINSNPAKPEKEW
                     IEDWEKTMASNLTSCAALCRKAINCFVKQGGGIIVTISSRAAFRGDTPDYMAYAASKA
                     GLVALTRSITRGYGKQGVVAFTVAPGFTRTEMAQVFIDKYGNDYATGDIALSELTEPK
                     DIAPTVLFL"
     gene            complement(697..1767)
                     /locus_tag="VGA21_05390"
     CDS             complement(697..1767)
                     /locus_tag="VGA21_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015812413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX9649973.1"
                     /translation="MDVSAQRREVDESLKMDFEEYDPVSTLVVPEHPMTKASFPFIDV
                     HNHQFRMPEMDLKELITEMDKLNMAVMINLSGRGRNSSDHLQKSLDNVMKNYPNRFIV
                     FTNIDLSTIDEAGWSEKTAKQIEDDYKMGARGLKIYKSQGMDNKDSNGDRIPIDDSRI
                     DPVWARCGELGIPVLIHSADPAPFWDEMNASNERWLELKLRSGRKRDSDDASWEQIMS
                     EQHHIFEKHPGTKFINAHLGWYANNLGKLAELMEKYPNMYAEIGAVIAELGRQPKFAN
                     EFITKYQDRIMFGKDSWVPEEYHTYFRILETGDEYFPYYKKYHAFWRMYGLGLSEEVL
                     KKLYYKNALSIIPGIDKTLFPD"
     gene            complement(2272..2811)
                     /locus_tag="VGA21_05395"
     CDS             complement(2272..2811)
                     /locus_tag="VGA21_05395"
                     /inference="COORDINATES: protein motif:HMM:NF024978.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4136 domain-containing protein"
                     /protein_id="HEX9649974.1"
                     /translation="MRPGRILILLFCHLAFSCNSIRVITHYNENSDFSKFHSFKTVND
                     IERPGNTGTYTEYLDLDRAIAAKMIERKYTEETDADLGVHYRIILNKKIDYNYNRDRL
                     YPYTNYETYGRKYTEGVLIIEMRDRDTRKVVWQASLDLKINKRKKLENVIEDTIDFIF
                     SEYPFEAGVKNLLFRKDNE"
     gene            complement(2850..3308)
                     /locus_tag="VGA21_05400"
     CDS             complement(2850..3308)
                     /locus_tag="VGA21_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009582036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649975.1"
                     /translation="MSFITKIFGKKEKKPKAKCNLSDTLLEFGEGYQLTTSQIVASRK
                     FWDHKMLEPETLSYTTAHFKNNDPTATKMREMIFNKYAMEEKPWLICESYIHLFEVDK
                     GQAQEYAKEWWTSGGQFSPPNSGSAKAIMGSTAFEEIREYATMVAGGERV"
     gene            complement(3455..3676)
                     /locus_tag="VGA21_05405"
     CDS             complement(3455..3676)
                     /locus_tag="VGA21_05405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649976.1"
                     /translation="MTYLIIFVSVVLALQIGMMFLIRWKKKKDKKENVLLRYNINTPG
                     DAWKCLNDPNVSDEDKLKIQEYYSKSLKN"
     gene            complement(3710..4669)
                     /locus_tag="VGA21_05410"
     CDS             complement(3710..4669)
                     /locus_tag="VGA21_05410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX9649977.1"
                     /translation="MKISGFTFVKNATKLFFPIKESIESILPIVDEFIVLVSEGDEDD
                     FTKEEIGKINSDKIKLIPSRWDKENFHQNTVYAHETDKAMKHCSGDWLFYLQSDEIVH
                     EKYLDQISRACAYYLDAREVEGFLFEYKHFWGDFNHYFQSHTWYPREIRIIRNRGDIH
                     SWRDAQSFRIFRQFNGTSEDYLRKAGTRKLNVARINCEIYHYGWVRPPWVMTTKTKSV
                     DPHLGAQLKDGYFDYGPLNKLELYSETHPNVLKPWIEKIFDWGEELQISGKRNKNRPP
                     YKHERLKYRLLSLIENTLLGGNQIGGFKNYNIIRDFAKDMDQT"
     gene            complement(4698..6068)
                     /locus_tag="VGA21_05415"
     CDS             complement(4698..6068)
                     /locus_tag="VGA21_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018343468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HEX9649978.1"
                     /translation="MYAIVDIETTGGNSIYHRIIEIAIYITDGQQIIDEYQTLINPEI
                     RIPPYIIAFTGISNEMVETAPIFEDVIERIEELTHGKIFVAHNVNFDYSFIKKEYYEL
                     GKTFRRKKLCTVRFSRNILPDMPSYGLGNLCDELGIPIHNRHRAAGDARATVELFHQL
                     IEKDETGFLEYSLKRDSKEALLPPNLPREQFDELPEENGIYIFHDEKGNPIYIGKAKN
                     IKDRVATHFAGNTNTNSRRLFLNSIFGVSYEVCGNELISLLYESFLIKKHWPRFNRSQ
                     KKVEYNYGIYQYEDRNGYYRLTVNKVSRWETPVATFRHLSEARGYLGKKVREFELCPK
                     LCGLQKAPHECYDHKIGTCHGACCGQESTQEYNSRFLRATENFSDVKKSFCIIGQGRS
                     RDEKSIVVVEEGCYLGFGYLDSSLQFTRPNELKDYIDSFPDNPDVQKILNGYLRKSSP
                     DQVIAY"
     gene            6241..6576
                     /gene="trxA"
                     /locus_tag="VGA21_05420"
     CDS             6241..6576
                     /gene="trxA"
                     /locus_tag="VGA21_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009581029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEX9649979.1"
                     /translation="MAVLSKPIHITDDTIEKVLSTTDKPVIIDFWADWCGPCHMIAPI
                     LEQVAEEFEGRALVTKLNVDENRISAEKYGIRSIPTLLFFKNGKLVDNQVGVVPKQMI
                     SDKLRKLSN"
     gene            complement(6637..8853)
                     /locus_tag="VGA21_05425"
     CDS             complement(6637..8853)
                     /locus_tag="VGA21_05425"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009583666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEX9649980.1"
                     /translation="MKKLFTALSMVFPLLGMSQGDYGKKFEQLETILPTPNTYRTASG
                     APGKDYWQQRADYEIEVELNDINQSITGSETITYHNNSPDILKYLWVQLDQNIREKDS
                     DKFTTGNSAIEPVMNGKSLLEITNGYDYEGGYKIISVKGTAGEDLPHVINKTMMRVNL
                     HVELKPGEKTSFSIQWVYNINDKMLDGGRSGYEFFPNDGNYVYAIAQFFPRMAVYDNV
                     VGWQNKQFLGRGEFTLPFGDYKVKITVPADHIVGATGLLQNPDETLTNAQISKFEQAK
                     KSDKPVIIVSQEEAIKKEKNPDTKNKSTWIFKADNVRDFAFASSRKFIWDAQKVDIGN
                     NAPLAMSFYPKEGNPLWEEYSTVTVAKTLIGYSKRTIDYPYPVAISVHVASAGMEYPM
                     ICFNRGRPFEDGTYSSSIKWSMIGVIIHEVGHNFFPMIINSDERQWTWMDEGLNSFVQ
                     FLVEREFYPDKPVTRGTPETIVPYMKGGKDMIRPVMTNSEQILQFGNNAYSKPAAALN
                     LLRNTVMGPDLFDYALKEYAQRWAFKHPTPADFFRTMEDASAVDLDWFWRGWFYSTDN
                     VDVSVENVKWFRMAGMEPGFEKRMKSGKVTANSDGTGAANSLSNGFEEFKVIETDTRF
                     YREFLNRVDDEDVIKKNTGKNFYEVTFKNIGGLVTPLVLEWTYADGSVEIEKVPAEIW
                     RYNENEITKVFTKEKQVINLKLDPMNELADTESSNNNFPREEDKSRFEQFKNKTKN"
     gene            complement(8855..9436)
                     /locus_tag="VGA21_05430"
     CDS             complement(8855..9436)
                     /locus_tag="VGA21_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010608716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HEX9649981.1"
                     /translation="MSEFGLYFQLGVEHILDTRGYDHILFIIALCVIYNAREWSRVLI
                     LVTAFTIGHSITLVLATLELISVRTDVVEFLIPVTILITATNNIFSKPSSGTRRIQVN
                     YLFALFFGLIHGLGFSNYLKSLLGTDSSVITQLFAFNIGIEAGQIIIVGIYMIVAFIA
                     NGIFYISRRDWVMVISSAIAGIAFILIIENKFW"
     gene            9505..9975
                     /locus_tag="VGA21_05435"
     CDS             9505..9975
                     /locus_tag="VGA21_05435"
                     /inference="COORDINATES: protein motif:HMM:NF042765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6702 family protein"
                     /protein_id="HEX9649982.1"
                     /translation="MMLIILLAVLVHPIHISVVEVRFSEDSKTFQLSQRIFVDDLEAA
                     INKENSTSLDILKLEDSVRNKILRRYLLAKIEIRLDNKKTVLNYIGSEMEEGVCWVYF
                     ESAKTKSFKQITLKNVALFELFDDQSTIIQIEKDKTVKSFRLFGEKVEEIIVFE"
     gene            complement(9959..10528)
                     /locus_tag="VGA21_05440"
     CDS             complement(9959..10528)
                     /locus_tag="VGA21_05440"
                     /inference="COORDINATES: protein motif:HMM:NF024952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HEX9649983.1"
                     /translation="MDKTVKENLFFIAIVPEEPLYSMIMNIKESFAKDYDSKAALRSP
                     PHITLHMPFKLSLEKAGKLKLRLVEVLLNFSPFEVKLKNYGSFPHKVIFIQVMESESL
                     TNLHSIIKRVMRTEFNFLNQDYKNQGFHPHITVAFRDMKKQEFNKAWEKFGVKNFEDQ
                     FACNSVFLLKHTGKIWEKYFEIRFLTQKQ"
     gene            10662..10991
                     /locus_tag="VGA21_05445"
     CDS             10662..10991
                     /locus_tag="VGA21_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014022741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjQ family protein"
                     /protein_id="HEX9649984.1"
                     /translation="MKRIHMIIVNTETIAGREIKENLGIARGSTVRARNIGRDIFAGL
                     KNIVGGEITEYTQLLADSREQALSRMIKDAEEMGADAIVNVRIATSAVMQGSAEILCY
                     GTAVKLR"
     gene            11014..12195
                     /locus_tag="VGA21_05450"
     CDS             11014..12195
                     /locus_tag="VGA21_05450"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX9649985.1"
                     /translation="MKSEISTFSPSGRSFQGQKFNKSIPGNSSALAVIGAGILGVCSA
                     YYLRKAGFNVTLFDKRGPGNSASSSGGESRLLRLIYGASQFYSRLTLDAIPLWMELQK
                     GTDPFFIPSPVLWISGKKNKKIILDSLGFLESNKIRYRELSREDLQKKWPFMQVSDIE
                     IAVLEELSGYLKARNACQAIASKFVEEGGNIIWDDVKPEKAGTEQLSGLKLSTGGVEH
                     FDYFVIAAGPWLNQWVESPVIEVTRQEVHFFDVPANSASMKSFTSWVDLSYEPYFYGI
                     PGDLTRGFKIACDKRGSLFNPDLDSRVPEIDLLQESRKFLRFRFPELAQLPLLESRVC
                     QYSNSFDRNLIMDVHPSYNNVLLLGAGSGHGFKYGPAIGKLVSEFIPGQKPLFNEMRL
                     F"
     gene            complement(12192..12572)
                     /locus_tag="VGA21_05455"
     CDS             complement(12192..12572)
                     /locus_tag="VGA21_05455"
                     /inference="COORDINATES: protein motif:HMM:NF021182.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HEX9649986.1"
                     /translation="MLNNELKFKVWNKVKKKMYISGSLSFSSERHSFVVFADKEMGLF
                     KDDDIEILQFTGQKDMNNREVYQRDIVEIEGKRFIVIWDNNRGGWSYTDVERKMISHT
                     FGRSEANNSKVIGNEFETPELATL"
     gene            complement(12609..13685)
                     /locus_tag="VGA21_05460"
     CDS             complement(12609..13685)
                     /locus_tag="VGA21_05460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649987.1"
                     /translation="MRKLLYIYILLVYISSPLIAQDILLTNSPVYLNVSFEIPEDLLS
                     TKTAVIVEVGSSGLNWKEISNYTQIQLRRTGIDAVAYFQWKTVFSGFEPQQEISEFLK
                     TRQVTNIILVRATKVKAEVWLTKFNNSWSVISSGQNAWYFSDSTVSKALLPLYQKAAL
                     EQERKNLLISDVPEYYEGLIFHKRKISKAYPPDVRGFKTGIALIPSTSKPEEINVNLQ
                     RVFETYQDAIMARNDELKNVITEKYPFKYEFYDFGLTMEELRTTKGCNFFLGYVYGRA
                     NDITQMFKLSSGDSKEIITNAVLSEKPIIRSIPADRMVFKFYFRNTVTGEIFIGSVWD
                     ADVAINDALRNFIFYMKRDLKVNP"
     gene            13825..14631
                     /locus_tag="VGA21_05465"
     CDS             13825..14631
                     /locus_tag="VGA21_05465"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX9649988.1"
                     /translation="MNDLRILSHDSMEGRKTGTDGNEKAREFILKRLREIAVKPLESD
                     FQQKFNFSASDGEKTGTNILGHIEGIASSNSYFVISAHYDHIGVRSGEIYNGADDNAS
                     GVAALLAIANYFKKNSPRHSMIFAFFDAEESGLQGAKAFVATNSRAIILNVNMDMISR
                     NDKNEIYIAGTYQNPDLKELVQKSLSQNDRLKVLFGHDTPGSGGHDWSNSSDHGPFSK
                     SGIPFLYFGVEDHEDYHKPGDDFEKIDINFYNSVVEMLVHVVNSLDKELE"
     gene            14642..15766
                     /locus_tag="VGA21_05470"
     CDS             14642..15766
                     /locus_tag="VGA21_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019596511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEX9649989.1"
                     /translation="MKRKFQVAIIGGGLGGLICANYLAREKIEVCLIERKSYPFHRVC
                     GEYVSNEVKPFLQSLELFPTHFNPPEINRFILTSINGKKAETDLRLGGFGISRFSFDH
                     LLSEKAKVNGAVILTNRFVDDIAFDSGTFKVSISGGKGIESDLVIGAFGKRSSLDKKL
                     SRPFLKHKSPYIGVKYHIHYELPKNEIQLHNFEGGYCGISNVENNTVNLCYLSERGNL
                     KKCGNIQSMEEKVLQKNPFLKEIWRNAEFVMNNPIVINEINFESKNQVESHILMVGDS
                     AGMIAPLCGNGMSMAIHAAKIACEEIVRFFGSPEITMTRLETNYMERWKNQFSSRMVI
                     GRNLQKLFGSVWLSNASVGLLNNFGTLGKFLINKTHGQPF"
     gene            complement(15943..16809)
                     /locus_tag="VGA21_05475"
     CDS             complement(15943..16809)
                     /locus_tag="VGA21_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013454363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HEX9649990.1"
                     /translation="MIKYSTIIHLRFPFSVFLLPIYLFALSQSPEVSYENAILIFIVL
                     HLLVYPASNGYNSYFDKDEGSIGGIKTPPKVTRQLYLAALVMDAVALIIAFFISWIFG
                     TGILIYGLASKAYSHPFIRLKRYPVIGWLTIGTFQGFITYLISFQGMNDLPIQETISD
                     GWQPAVLATVLLLGSYPMTQVYQHEEDEKRGDITISYKLGLLGTFHFTGILFGIATLC
                     FFLFFLKNHSAITAALFLGCMLPTVIFFGVWYFQVRINPQKADYRNTMTLNLISSIGL
                     SLFFLLITFFDM"
     gene            16855..17949
                     /locus_tag="VGA21_05480"
     CDS             16855..17949
                     /locus_tag="VGA21_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007660504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III polyketide synthase"
                     /protein_id="HEX9649991.1"
                     /translation="MGSYIKAIGTANPGQPIPQKSIASFMVKAHGLEGQEQSKLEALF
                     RSTGISSRYSVISDYSGNGQRNFFPKTNNLEPFPSTKMRMDLFRNKAVDLSLRAVYDC
                     LGNKFQLNQITHLITVSCTGMYAPGLDIDLVTQLGLPSTTSRSAINYMGCYAAINALR
                     IADSICKSDVAAKVLVVCVELCSIHFQKVNSEDNYLANSIFGDGAAAVLISSGMTKEM
                     DLELLSFYSDILDYGKEEMAWTIDDFGFQMKLSSYVPDVIRKGIAELTYRFLSGNNLE
                     LKDIDFFAIHPGGKRILDEIESELGMEKSKNQYAREVLKNYGNMSSPTVLFVVKSLLS
                     IIKKNDKGKIILVMAFGPGLTIETVLLRVV"
     gene            18132..18731
                     /locus_tag="VGA21_05485"
     CDS             18132..18731
                     /locus_tag="VGA21_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002694517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX9649992.1"
                     /translation="MSNLRLSDSELISLYKNGNEEAFESIVNRHKRKVYTSIYLIVKD
                     SYVAEDLLQETFIKVVKTVKSGRYNEEGKFLPWLMRIAHNLAVDFFRKEKRYPTIIME
                     DGDKIFNNLQFSEDSFESKQLVTDTHNKLKFMIKELPETQREVLVMRHYMEMSFQEIA
                     EATGVSINTALGRMRYALINLRKKVRKYNIAYDKNIYPE"
     gene            18706..18945
                     /locus_tag="VGA21_05490"
     CDS             18706..18945
                     /locus_tag="VGA21_05490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9649993.1"
                     /translation="MTKTFTPNELIRYYYKETSEKENRKILNALICDPVLQEQYKEIS
                     KVIRQLDDVKFNPSDKVIKAILDYSRNADVHSLNK"
     gene            18945..19592
                     /gene="nth"
                     /locus_tag="VGA21_05495"
     CDS             18945..19592
                     /gene="nth"
                     /locus_tag="VGA21_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013454307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEX9649994.1"
                     /translation="MLRKERYEAFVRHFTKHQPEATTELNYTNPYELLVAVILSAQCT
                     DKRVNLVTPSLFKKYPTPKHLAVSGFDELFPLVKSISYPNNKTRHLIGMAKLLVEKFN
                     GRVPVELKHLQELPGVGRKTANVIASVIYDQPFMPVDTHVFRVSKRLGLVRQNSKTPL
                     DVEKQLMKCLPNDVISVAHHWLILHGRYVCLARKPKCNLCNISHFCRYFEKLKTS"
     gene            19589..20764
                     /locus_tag="VGA21_05500"
     CDS             19589..20764
                     /locus_tag="VGA21_05500"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX9649995.1"
                     /translation="MKILIIHQHFRTPNQGGSIRSYHIARELSLLGNQVSVISSSQKA
                     RNYVTKVENFEIRYLKVRYENSFGFARRMISYSSFLLKAIYSLKLYKDYDVCYVMSTP
                     LSVGIIGLWAKSHYKIPYVFEVGDLWPDIPIQMGIIRNPIFKWSVLKLEKLIYSKSKF
                     IVALSNEMKQLIQVKVKDKQITVIENFADSAFFKIKNVEIKIPSTIKKGNVVISYIGT
                     LGIANKVETLIKLAGILREEAITIVIKGSGKELQLCQGLANKLNLHNVIFMPFGSKDE
                     IKELLSYTDAIYVSFDKRYPLLTTGCPNKLIDGIAAGNIVLMNYGGWQKELIKNTKCG
                     IIMDIDNPADCLNKARSILLEPGKREVMKNNSRMLAQKFEVKLLVERLYLEIAKALN"
     gene            complement(20761..21759)
                     /locus_tag="VGA21_05505"
     CDS             complement(20761..21759)
                     /locus_tag="VGA21_05505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility lipoprotein GldB"
                     /protein_id="HEX9649996.1"
                     /translation="MIYKGSLFLVICTFLQSCANDDCEVDESISGITVNIEISRLEET
                     MFSLQSSEEVRDFLNENSAMKIHFLASDQYPDDSILINNLFRRIKDPHIDTLLREVRQ
                     EFADFSDLKLELEAAYRYLKSFYPEILIPKVQTMVTGFGSSELYVSDSLIIIGLDYYI
                     GPYATYRPIEIPNYILKRYQKEYIVPAIILLLANKFVKDDYSDRTMLADMVYYGKKYE
                     FAKSIMPCAPDSLIIWYSGQQLTDVNENEDIIWANFVANQLIFETNHMTKTKYMDERP
                     TVFEIGDKCPGRIGAWLGWQIIRSYREKNPDISVHQMMEIQSATAIFNNSGYKPSR"
     gene            21836..23098
                     /locus_tag="VGA21_05510"
     CDS             21836..23098
                     /locus_tag="VGA21_05510"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HEX9649997.1"
                     /translation="MKLRLIISLFIYSHLGICQSVLDSLTRSEERNYNKISYQSLRVP
                     SSISTNVSLLADYLTQEAKTELEKVRAFYVWITSNIAYDFRSLEKNLYPVQTPEVVIA
                     RRTALCQGFADLFHELCEEVDIRSAVISGYSKGYGYKPGKEFFVSDHAWNAVRVNNLW
                     YLLDATWGAGSGITQKSGLEDGFNDKYFLTPPEKFVEDHLPLDPMWQLLNNLVPLQVY
                     EKGEAEINAFMETAHKTFNFKDSLMRWESSDVYQVAIESHKRSITFNPKNKRAVYELG
                     TELLFKALDVMDGIHGVSYGDFDNLVEEMRKSMFDLLHEAAFHFSNIPSSHPDYETAS
                     TLLEEVTFQKGVFYYEVGQRIYDIFLEMDREAFYRNLANGILQIDNYYDLATYYFKLV
                     NEESRYFPQAREYIQNYIINKNPLRPGN"
     gene            complement(23095..24645)
                     /locus_tag="VGA21_05515"
     CDS             complement(23095..24645)
                     /locus_tag="VGA21_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018472198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HEX9649998.1"
                     /translation="MSSELIINSTQNGCRIALLKDKGLVEFHIDEDGTQFNVGDLYLG
                     TVKKVVQGLNAAFIDIGHEKDAFLHYLDLGPQFASLSKFTKLVLSNKNISGDLGNFRN
                     EPDINKFGKINQVLTRNQKILVQVVKEPISTKGPRLSCELSMAGRYLILVPFSKVVSI
                     SRKISSADERKRLTRLVTSIKPENFGVIIRTAALGKDVAELDRDLRNLVNTWKEGIKK
                     LRNAKPREKVIGEMSKASSILRDVLNESFDNILVDDPKIYDEVKGYIKRIAPGKEKIV
                     KQYSGKAKIFEQFGIEKQFKSLFGQTISLKAGGYLIIEHTEALHVIDVNSGNKSNREV
                     DQEATALSVNLEAAAEIARQLRLRDMGGIIVIDFIDMRKSDNKKLLQKKMKEEMSADR
                     SKFTVLPLSKFGLMQITRQRVRPEMNITTLETCPTCNGTGKIKASILVSDIIETSLDH
                     LIIKQNEPAISIAVHPYIHAYFTNGVISRQMKWFFKYRKWITLIEDSSLGITDFKFLN
                     KEAEVIEI"
     gene            complement(24780..25589)
                     /locus_tag="VGA21_05520"
     CDS             complement(24780..25589)
                     /locus_tag="VGA21_05520"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9649999.1"
                     /translation="MWKNRVILLLVGIALIAILYSLPKVVVDNKPEEITPESAQQGTG
                     FHQSKASPEEHQRVLDFKTQLEKTDNIEKSSIFADSLAEAFRAMNQWDSAAYYLGRQA
                     ERFETPDNLEKAGEAYFQAQGFAISQNEIEMLGQQARMFFDKVLVQNPQDLEVKTKIG
                     LTYIASSNPMQGIIVLREVLEVDPNNEMALYNMGILSMQSSQWERAVERFTKLIAVNP
                     ENSRAHFYLGLSYLELDQKQLAKEHLLKVKELEKDPEVLANVDSYLEDIEN"
     gene            complement(25613..25918)
                     /locus_tag="VGA21_05525"
     CDS             complement(25613..25918)
                     /locus_tag="VGA21_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007655567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEX9650000.1"
                     /translation="MTKADVITEIAEKTGIDKADVTITVEAFFNVIKNAMSDGNNIYV
                     RGFGSFINKRRAKKIARNISKNTAIVIDEHYIPSFKPSKVFVEKIKKSDKVKLVNSK"
     gene            26057..27124
                     /gene="mutY"
                     /locus_tag="VGA21_05530"
     CDS             26057..27124
                     /gene="mutY"
                     /locus_tag="VGA21_05530"
                     /inference="COORDINATES: protein motif:HMM:TIGR01084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEX9650001.1"
                     /translation="MKYKPLSVNYFARRVLQWYYRAKRDLPWRNERDAYTVWVAEIIL
                     QQTRVEQGLPYFNRFIETFPDVFSLAKASEQSVLKIWEGLGYYSRARNMHKCAQIIVN
                     NNHGSFPKRFEDLLQLPGIGKYTAAAIASFAYHEAVPAIDANVIRVLTRIFRIHEDPH
                     KPKTQKLLFEKSLKLISKDNPDHYNQAMMELGALICKPKNPSCARCPVMDCCEAYASG
                     DQQKYPYKKARGKIRKRYFNYVILKSGDKTYFQLRNGDDIWKGLYEFFLIESKAPIDI
                     SDILHKVPIELLEEPRVLYESKEYKHQLSHQEIRARFIEISSPGIQTKQWLKPFLFKK
                     KMVKDLPKSVLLNKYLEERPI"
     gene            27163..27579
                     /gene="ssb"
                     /locus_tag="VGA21_05535"
     CDS             27163..27579
                     /gene="ssb"
                     /locus_tag="VGA21_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013454711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEX9650002.1"
                     /translation="MAGVNKVILVGNLGKDPEVRHLEGGAAVANFSLATSESYKDKTS
                     GERKTITEWHNIVLWRGLAEVAEKYLKKGDSVYIEGKIRSRQWEDKEGKTRYTTEIVG
                     DNMVMLGSRGGSSSGSQSAEKDIVTSDADETDDLPF"
     gene            27741..29045
                     /gene="gldE"
                     /locus_tag="VGA21_05540"
     CDS             27741..29045
                     /gene="gldE"
                     /locus_tag="VGA21_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009581719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated protein GldE"
                     /protein_id="HEX9650003.1"
                     /translation="MITPLVIGYGVSIILLLILSALISGSEVAFFSLSNQDIIDMRKN
                     PTESGKRTMVLLQNPKRLLATILILNNLVNIAIVTLSTFLTWELVGTHSPEGVILIIL
                     TSSVTFAIVFFGEVMPKVFAVQNYLLFARITAPLLWISTRVLKPVAWVLMNLSGVIEK
                     RFEKKGYSISVDELHQALEIATDKNTSDEEKEILKGIVNFGTLTVKQIMRSRLDITAV
                     DEDCNFHELLDKINKSGYSRIPVFNETIDSISGVLYIKDLLPYIEEEENFEWQKLLRP
                     GIFVPETKKIDSLLKDFQEKRVHMAIVVDEYGGTSGLVTLEDIIEEIIGEINDEFDEE
                     DFIFHKLDEQTFVFEGKTSLNDFCKAIDVDVSVFDDIKGESESLGGLILEINSTLPQA
                     GEKIYFDNFVFTIVAVDQKRIKRVRVFIKEKAREGKSTEFTD"
     gene            29014..29655
                     /gene="gldD"
                     /locus_tag="VGA21_05545"
     CDS             29014..29655
                     /gene="gldD"
                     /locus_tag="VGA21_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009197490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility lipoprotein GldD"
                     /protein_id="HEX9650004.1"
                     /translation="MKENQRSSLTNSWAILLFSFVLLAAVGSCTKDWLPKPRGYNRLP
                     LPEHEYRLLPDSFPYIFEYSAHARIYNDSSWIAEPYWIDLFYPDLIANVQITYKPVNG
                     NQKLLEEYLSDAYKLTSKHQIKAYSIDESYLITPTGKTAVLAELGGEVPSQFQFYVTD
                     SVEHFLRGALYFRTSLKNDSLSPAIEYVKIDIIHMLNTLQWRTEVPKNVSLRQ"
     gene            29659..31752
                     /gene="recG"
                     /locus_tag="VGA21_05550"
     CDS             29659..31752
                     /gene="recG"
                     /locus_tag="VGA21_05550"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010611623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEX9650005.1"
                     /translation="MANFFDTGIEFIKGVGPQKASLLEKELNIRTFGDLLTHYPFRYE
                     DRTRFYSIKDLNESLPYVQIKGTLKALYTLGGGKRKRLVGYIVDESGEIELVWFKGIN
                     WVTKKLKIGLEYVVFGKPTLFAKKLNIAHPEIEVLTETSSKISYLQPVYPTTEKLKSK
                     FLDSKAISKIQITLLKAALPKMGESLPSGLLNHYGLMPKKKALRHIHFPSNQNMLSKA
                     LYRLKFEELFFIQMKLLMMKVSRMEKFKGHAFNSAELVTEFYEKHLPFELTRAQKRVV
                     KEAYRDMRSGHQMNRLLQGDVGSGKTIVAFLCMLLAINGGTQTALMAPTEILANQHYK
                     GLRTFAKKMNLRIALLTGATNTKDRKVIHQYLANGDLHILVGTHALLEDVVQFKNLGL
                     AVIDEQHRFGVAQRSRLWEKNAVLIPHVLVMTATPIPRTLAMALYGDLDISVIDELPA
                     GRKPIITRHQYDSNRLELFGFIRKQIGEGRQVYIVYPLIEESEKSDLKDLMDGYESVR
                     RAFPEFQVSILYGKMKSDAKDFEMSRFQKRETQIMVATTVIEVGVDIPNASVMIIENA
                     ERFGLAQLHQLRGRVGRGADQSYCILMTRYELGQDARTRIDTMVRTNDGFEIADVDLQ
                     LRGPGDLLGTQQSGLLDLTVADLSKDGKILQVAREAAIAILKKDPELKSGENEYLNQK
                     LKSISETFVNWSRVS"
     gene            31776..34442
                     /locus_tag="VGA21_05555"
     CDS             31776..34442
                     /locus_tag="VGA21_05555"
                     /inference="COORDINATES: protein motif:HMM:NF019136.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triple tyrosine motif-containing protein"
                     /protein_id="HEX9650006.1"
                     /translation="MKKIFLFFAGVLFSISYLPAQHGHFYLTNHVPRTVTFENTNFKI
                     IQNSQGILYIANNRGLIAYDGTNWDFIETPEAIFSIAIDQTDQIFIGGQSGFGRLIKV
                     SRNEFLYESLSDSVVSANEIFEIKEHLGKIYFLNQKAVFIYESGKISELRLKDASVFR
                     TFAPLPQELLAITNDGRVLSISESLKSFDPGIGYDVTLETSFLSPDKSKAILIDSDGN
                     LFLYENRTAAKIVFNDKELLSKFIVTSGVWLEGDILAIGSLESGVVFLDLNKKTLLEV
                     LNYESGLPDNQIFSMTRDRVENSLWISHAYGFSRVSPFLPFHSYGRFPGINGKLLTGY
                     FGGNQIYLGTSVGLFRLQEVREFREKVIYVPVRTSTGPTSVQEAEKLQDEKKSRRGLF
                     GFLRRKEEEEKEAVVEQQQIKLQRRVVQEETSREWKYKKIEGIHSRVMKLVGTNGGIV
                     VASLSGLFEVKGNNEIPISSEPFTFVHAMKGIDGVIAAGANGGVSFFRKSGDNWSSYP
                     TFSEIEEYIQFIFQDSKNNIWLLGPNVAYKATLFQDVIDNLEFWPIENPFYSTNIGGE
                     INGQVYILNSNSLYTFNEITKEIEKKESTNLWSKFPERTISDLDGTIWIYDGKDWSPL
                     GSQYLTSRKIRPISLFNEVSFLTYDKDSDSFWIITGENDLYKMSNKDTIVSNHLPLLL
                     REIKNGQRLLPIEGNFEIQQNQNSLGFNFLKADFDNLFKIEYRYKLEGLNASWSEWSG
                     DFNNISFPFLPEGNYVLNFESRDVFGQINSLEPFTFKIVPPYWKRPWFYGVEIIAFGG
                     LFLLSFRLNRIDHKYKFLSKLLAFLTLILAVEFIQIVAELKFPSGNSPVTGFFIQVFI
                     ALVLLPLEGVVRRAVFRIEETQDMKAFNKKAK"
     gene            34500..35282
                     /locus_tag="VGA21_05560"
     CDS             34500..35282
                     /locus_tag="VGA21_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020527212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650007.1"
                     /translation="MKSFLIAALILFLGAGCQTEKQSQTITEENPPMEDFNFEESDSN
                     AIVIADSVMKKMGGRKNWDETRYISWTFFGRRKLLWDKWDGNVRIEFLNDPSTIYLVN
                     VNSMQGKAMKAGVEITHPDSLAKELERAQALWINDSYWLFMPFKLKDSGVTLKYIRQD
                     STITGTLSDVLQLTFKNVGNTPNNKYEVWVNRDEGLITQWAYFQNSAQDSASFIRPFD
                     NYQKYGKILLSGDRSDNGGPGNIKVMESVPEEAFTSFDFSHQ"
     gene            35454..35924
                     /locus_tag="VGA21_05565"
     CDS             35454..35924
                     /locus_tag="VGA21_05565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650008.1"
                     /translation="MERDVSKLLKAQLKKLNSKDFDLEAWKSSTSAVLSIAFGADDPH
                     VHEIQKLKIDYGSWALRDAKASYDPMVTCKKIGKEVVEIALSQLGDREELTSDLNSIL
                     ESQLMDKSLKEIRKVLKSSKSDSKKGEKLQEILKGLDNDNLASILAQMILKSEN"
     gene            complement(35950..36135)
                     /locus_tag="VGA21_05570"
     CDS             complement(35950..36135)
                     /locus_tag="VGA21_05570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650009.1"
                     /translation="MKDTRSEILLSLESLNKDQQVKVLSFIRNFTSPQKAGSDDDFKI
                     RALREIQLALKSKIFLV"
     gene            36363..36929
                     /gene="msrA"
                     /locus_tag="VGA21_05575"
     CDS             36363..36929
                     /gene="msrA"
                     /locus_tag="VGA21_05575"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018474613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEX9650010.1"
                     /translation="MKLNNMKTEVATFGAGCFWCVEAIFQSLKGVEKVVSGYSGGKIK
                     NPSYREVSSGLSGHAEVVQITYYPDIIAYKELLEVFWQTHDPTTLNRQGADVGTQYRS
                     VIYYHDEEQKNAAEFYKQELTKAGVFLNPIITEIKKFEGFFIAEEYHQNYFMLNSDAP
                     YCNYVIKPKMEKFKKAFAEKYKKAEIDN"
     gene            complement(36975..>37601)
                     /locus_tag="VGA21_05580"
     CDS             complement(36975..>37601)
                     /locus_tag="VGA21_05580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650011.1"
                     /translation="RFLIWVENLGDIANEFRFNEVRPREMFTRKQFFLTEKLKKSDHP
                     LARISWEPISQIRTGRIHPTPPDSFYKILNGTWQGSFADKEIRISIYPVDNVNPERKL
                     TGFNKFTTQDDIKQIDMRGTFGDSGDHFEVTMEEYRLPYDEEPAWKREIIEKYWGGPE
                     KFYPAESSYGVFNVTVDKKSQVMKGTWKSNNGQLIREFEIPKISEGTN"
BASE COUNT        11344 a         7183 c         7296 g        11778 t
ORIGIN      
        1 aagaaaaagt actgtgggcg caatgtcttt aggttctgtt aattcagata atgctatatc
       61 accagtagca taatcattac catatttatc tatgaaaacc tgagccattt cggtgcgcgt
      121 aaaacctgga gcaaccgtaa atgccactac gccttgtttg ccataacctc ttgtaataga
      181 tcgagtgagg gctaccaggc ctgcctttga tgcggcataa gccatgtaat ccggtgtgtc
      241 accacggaat gctgcccggg atgaaatagt tactatgatt ccgccgcctt gctttacaaa
      301 acaatttatt gctttcctgc aaagagctgc gcaagaggta aggttggagg ccatggtctt
      361 ctcccagtct tcgatccatt ctttttcggg tttggcagga tttgaattaa tggcaattcc
      421 cgcgtttaaa atcaaaacat ccaagccgcc tgaaaatgca ataaactcat caataattcc
      481 tgaagacttc ccaggattgg agaagtctgc ctgcaatgca attccttttc cggtaattag
      541 agcttcagca tctttcttgc tggcattgta atgggcccca acaatagacc cctcacccac
      601 gaatgccttt gttattgctt taccgattcc tcttgaggag cccgtaatta agactttttt
      661 attctgaaga tttaattcca tctgggatac taaaatttaa tccgggaaga gagttttatc
      721 aatgcccgga ataatggaca gcgcattttt gtaatacagt tttttcagca cttcctcaga
      781 aagtcccagc ccatacattc tccaaaaggc atggtatttt ttgtaataag gaaaatactc
      841 atcccctgtt tccaaaattc tgaaataggt gtggtattcc tccggtaccc aggaatcttt
      901 tccaaacata attctatcct gatattttgt aatgaattcg ttggcgaatt tgggttgcct
      961 tcccagttct gcaattactg ctcctatttc cgcgtacata tttggatatt tctccatcag
     1021 ttcggccaat ttgccaaggt tattggcata ccagcccagg tgggcattta tgaattttgt
     1081 ccctggatgt ttctcaaaaa tatgatgctg ctcactcatt atctgctccc aggaagcatc
     1141 atcggaatct ctttttcttc ctgaacgcaa tttaagttcc agccaccgct cgttactggc
     1201 attcatttca tcccaaaaag gcgccggatc cgcggagtga attaataccg gaatccccag
     1261 ctcaccgcac ctggcccaaa ccggatcaat ccttgaatca tcaataggaa tcctgtcgcc
     1321 attactatct ttgttatcca ttccctggct cttgtaaatc tttaatcccc gcgcacccat
     1381 tttgtaatca tcttcaattt gctttgcagt tttttcagac caacccgctt catcaatggt
     1441 actcaaatca atattcgtaa atacaataaa tctgttggga taatttttca ttacattgtc
     1501 cagtgatttt tgcaaatgat ctgaactgtt tctgcctcgc ccgcttagat ttatcataac
     1561 tgccatgttc agtttgtcca tttcagtaat taattctttg agatccattt cgggcattct
     1621 gaattgatga ttgtggacat ctataaaagg gaagctggct tttgtcatcg gatgttccgg
     1681 cactaccagg gttgaaacag gatcatattc ctcaaaatcc atctttaatg attcatccac
     1741 ttccctgcgc tgtgctgata catccataaa caccagtagt gttagcgatg ttattatggt
     1801 ctttttcatg ggtataactt tggaaatcaa taaagataca atattcgatc tttcgggaag
     1861 aattgttaca gtgagttttc catgaatgga ataaaaaaac gaccttgatt taatcaaggc
     1921 cgtttcgttc ccatgagcag ttatcaacac gaagggacgg tgtcccaaca attaataact
     1981 gatgctcaaa agtattgttg aattagaact taagatggta aaattgtccc atttactggg
     2041 aatttttcat tgttccaggg atgccattct cctgaaacag aaagtattgg agattatgct
     2101 tattccccgt tgaccccggt gaatccccgg atcacccgtg actgaccacc aaaaatatca
     2161 gcaaatatca tcatcaagta ctacaataac cttctttggt gattggactc cagttaaaac
     2221 tggaaattgc ttaattcaat gatgagcatc tgtcaaaaaa gatagttgac attactcatt
     2281 atctttcctg aataacaggt tttttacgcc ggcctcaaaa ggatattcgc taaagataaa
     2341 atctattgtg tcttcaatta cattttccaa tttctttctt ttattaatct ttaggtccaa
     2401 acttgcttgc cacacaactt ttcttgtatc cctgtcccgc atttcaataa tgagtacacc
     2461 ttccgtatac tttctgccat acgtctcata attggtatat gggtaaagcc ggtcccggtt
     2521 gtagttataa tcgatctttt tattcagaat aattctgtaa tgaacaccga ggtctgcgtc
     2581 cgtttcttca gtatattttc tttcaatcat ttttgcggca attgccctgt ccaaatccag
     2641 gtattcggtg tacgtacctg tattaccggg cctttcgata tcatttacgg ttttaaaaga
     2701 atgaaacttg ctgaaatcgg aattttcatt atagtgggta attacccgga ttgaattgca
     2761 ggagaaagct aaatggcaaa acaagagtat caatatccgg cctggccgca tataacaaat
     2821 atacaaattg agttttgttt ttgttgatgc tatactcttt ctcctccagc caccatggtt
     2881 gcatactctc tgatttcttc aaaagcggtg cttcccatga ttgcttttgc tgaacctgaa
     2941 tttggcggcg aaaattgacc tccggaagtc caccactctt tagcatattc ctgagcctga
     3001 cccttatcta cctcaaacaa gtgaatgtaa ctttcacaaa ttagccatgg tttttcttcc
     3061 atagcatatt tattgaaaat catctcacgc atttttgttg cagtcggatc attattctta
     3121 aaatgagctg tggtgtaaga tagagtttcc ggttcaagca tcttgtgatc ccaaaatttc
     3181 cttgatgcga caatttgtga agttgttaat tgatagcctt ctccaaattc caaaagtgtg
     3241 tcagaaagat tgcactttgc ttttggtttc ttctctttct ttccaaaaat ctttgtaatg
     3301 aatgacataa ttctaaatag ttttgagttt tcttcgctta taattttacg ttcaaaaatc
     3361 aattaagttg tttgtcatta gacaaaatgg caaactggaa agtttcaaag ttacttagat
     3421 atacagtgaa aatgcggata ggttacatta aatcttagtt tttgagcgat ttagagtaat
     3481 actcctgtat tttcagcttg tcttcatcag aaacattggg gtcattcagg catttccagg
     3541 catctcctgg ggtattgata ttatagcgaa gcaatacatt ttctttttta tcttttttct
     3601 tcttccaccg gatcaaaaac atcatcccaa tttgcaaggc aagcacaacg gaaacaaaaa
     3661 taattaaata agtcatctca caattcttaa ttatagaaag gtacaaaact caagtctggt
     3721 ccatatcttt cgcaaaatcc ctgataatat tgtaattttt aaatcctcca atttgatttc
     3781 ctcccaggag tgtattctca atcaaactta gtaaacgata ttttaatctt tcatgtttgt
     3841 aaggtgggcg gtttttattt cttttaccac ttatttgcag ttcctctccc cagtcaaaga
     3901 ttttttcaat ccagggcttg aggacgtttg gatgagtttc actatacaat tccaatttat
     3961 tcaaagggcc ataatcaaaa tacccatctt ttaattgtgc acctaaatgc ggatccaccg
     4021 attttgtctt tgttgtcatt acccatggtg gtcttaccca gccataatga taaatttcgc
     4081 aatttatcct ggctacattt aacttccttg ttcctgcctt tctgagataa tcctcagagg
     4141 tgccattgaa ttgacgaaaa attctaaaac tctgagcatc gcgccatgag tgaatgtcgc
     4201 cgcgattccg gataatcctt atttcccgtg ggtaccaggt gtgtgattga aagtaatgat
     4261 tgaaatcacc ccaaaaatgt ttgtattcaa aaagaaatcc ttctacttcc ctggcatcaa
     4321 gataatatgc acatgccctg gatatctgat ccaaatattt ttcatgaact atctcatccg
     4381 attgcagata gaaaagccag tctcccgagc agtgtttcat agctttatct gtttcatggg
     4441 cataaacagt gttttggtgg aagttttctt tatcccaccg ggaaggtatg agtttgattt
     4501 tatctgaatt tattttcccg atttcttctt ttgtaaaatc gtcttcatca ccctccgaca
     4561 ctaaaacgat gaattcatcc acgatgggaa gaatcgattc tatggattct tttataggga
     4621 agaagagctt cgttgcattt ttgacaaatg tgaatccgct tattttcaaa tcacaaaaat
     4681 tattttagtg aactcattta atacgctatc acctggtccg ggctgctttt tcttaagtat
     4741 ccatttagga ttttttgcac atcgggatta tccggaaatg aatcaatata atctttcaat
     4801 tcatttggtc tggtgaattg taaggaggag tcgaggtatc caaaaccaag gtagcagcct
     4861 tcttcaacta caacaattga tttctcatcc ctgctgcgac cctggccgat gatgcaaaat
     4921 gatttcttaa catccgagaa attttcggtt gctctcaaaa atcttgaatt atattcctga
     4981 gttgattctt gtccgcaaca tgccccatgg caagttccga ttttgtgatc ataacattca
     5041 tgcggcgctt tttgcaaacc acatagcttt ggacaaagct cgaactcacg aacttttttc
     5101 cccaggtaac cccgggcttc cgataagtgc cgaaaagtag ccacgggcgt ttcccaccta
     5161 cttactttgt tcacggttaa cctatagtat ccattgcgat cttcatactg atagatacca
     5221 taattatact ctactttctt ctgagatcga ttgaatctgg gccagtgctt cttaattaaa
     5281 aatgattcat acaaaagtga aatcaactca ttgccgcata cttcataact aaccccaaaa
     5341 attgagttta gaaataatcg tctggaattt gtgtttgtat ttcccgcaaa atgtgtggct
     5401 accctgtctt tgatattttt ggccttgcca atgtaaatcg gatttccctt ctcatcatga
     5461 aatatataaa taccattttc ttccggaagt tcatcaaatt gttctcttgg aagattgggt
     5521 ggcagcaaag cttctttcga gtctcttttt aatgaatatt ccagaaatcc ggtttcatcc
     5581 ttttcaatta actgatgaaa taattcaacc gttgcccggg catcacctgc agctctgtgc
     5641 cggttatgaa taggaattcc caactcgtcg caaagattcc ctaatccgta ggaaggcata
     5701 tccggcaaaa tattccggct aaatcttacg gtgcaaagtt tcttgcgccg aaaggttttt
     5761 ccaagttcat aatactcttt cttaatgaat gaatagtcaa aatttacatt gtgcgcaaca
     5821 aagatcttcc catgagtcag ttcttcaatt ctctcaataa catcttcaaa aatcggtgcg
     5881 gtttcaacca tctcgttact tattccggta aatgcaataa tataaggtgg aatccgtatt
     5941 tctgggttga taagtgtttg atactcatca attatctgct ggccgtctgt tatgtatatg
     6001 gcaatttcga taatccggtg ataaatggag tttccccccg tggtctcaat atcgacaatc
     6061 gcgtacactc gttttgaaga ttttagcccc taatttaaga acttttaacc agccgtgttt
     6121 gcaataaatt catccgcaat agattcatcc tgatttccta tcaacaaaaa aaatccctgc
     6181 aagaaagaac cttaatctgc taattttcgt tataattgta acaaataaaa ttacaattaa
     6241 atggctgtat tatcaaaacc gattcatata accgacgaca cgattgaaaa ggtgttatcc
     6301 accacggata agcctgttat tattgatttt tgggcagatt ggtgtggccc ttgtcatatg
     6361 attgcgccga ttctggagca ggtggctgaa gaatttgagg gaagggccct ggtaacaaaa
     6421 ttgaatgtag atgaaaacag aattagtgcg gaaaaatatg gaataagaag tattccaacc
     6481 ctgctttttt ttaagaatgg caaattagtt gacaatcagg ttggagtagt accaaaacaa
     6541 atgattagcg ataagcttcg aaaattatcc aattaatatt ctttttgtgg tggtatggtt
     6601 tgggaaacgg cagagtgtgc gactctgccg ttctatttag ttcttagtct tatttttgaa
     6661 ttgttcaaac ctggatttgt cttcctctcg tggaaagtta ttgtttgaag attccgtatc
     6721 agcaagttca ttcattggat ctaacttcag gttgattacc tgtttttctt tggtgaagac
     6781 ttttgttatc tcattttcat tatacctcca aatctcggca ggaacttttt caatctccac
     6841 agatccgtca gcataggtcc attcaagaac taaaggagtt accagcccac caatgttttt
     6901 aaaggtaact tcataaaaat ttttgccggt attctttttt atcacatcct catcgtctac
     6961 gcggtttaga aattccctgt aaaatcttgt atcggtttca ataaccttaa attcttcaaa
     7021 tccgtttgaa agggaattgg cggcaccggt tccatcggag tttgcagtca ccttgcctga
     7081 cttcattctt ttctcaaaac ccggttccat gccggccatc cggaaccatt tcacattttc
     7141 aaccgacaca tccacattat cggttgaata aaaccagccg cgccagaacc aatccagatc
     7201 aactgcagaa gcatcttcca ttgttctaaa aaagtcggcg ggggtcgggt gtttgaatgc
     7261 ccacctttgc gcgtattctt ttaatgcata gtcaaacagg tcggggccca tcacggtatt
     7321 tcgtaacaaa ttcaaagctg cagcaggttt tgaataagcg ttatttccaa attgaagtat
     7381 ttgttctgag tttgtcatga caggcctaat catgtcctta cctcctttca tataaggaac
     7441 tatcgtctcg ggagtacctc gggtaaccgg tttgtccgga taaaattccc tctcaaccag
     7501 aaattgaaca aaactgttta atccttcatc catccaggtc cactggcgtt catccgaatt
     7561 aattatcatt gggaaaaaat tgtgaccaac ttcgtggatt attaccccga tcattgacca
     7621 ctttattgaa gaggaatagg ttccatcctc aaacggcctc ccccgattaa aacaaatcat
     7681 aggatattcc atacctgccg aagcaacatg aacggatatg gcaaccggat aaggataatc
     7741 aattgttcgt ttagaataac caatcaaagt tttcgcaact gttaccgtgg agtattcctc
     7801 ccacaaagga ttaccctcct ttggatagaa tgacatggcc aaaggagcat tatttcctat
     7861 atcaactttc tgtgcatccc agataaactt acgtgatgac gcaaaagcaa aatcccgcac
     7921 attatctgcc ttaaaaatcc aggttgattt gttttttgtg tccggattct tttctttttt
     7981 aatcgcttct tcctgagata ctatgattac aggcttatca gatttcttcg cttgttcaaa
     8041 ttttgaaatc tgggcattgg tgagtgtttc atccggattt tgaagcaatc ctgttgctcc
     8101 tacaatgtga tcagccggaa ctgtaatttt aaccttatag tctccgaatg gaagtgtgaa
     8161 ttcccctctt cccaggaatt gtttgttttg ccagccaacc acattatcat acactgccat
     8221 gcgtggaaaa aattgggcta tggcataaac ataatttcca tcattgggaa agaattcata
     8281 acctgagcgt cctccgtcca gcattttatc atttatgttg taaacccatt gaattgaaaa
     8341 ggaagttttc tctccgggtt ttaattccac gtgcagattc actcgcatca tggttttgtt
     8401 aattacatga ggcaagtcct cccccgcagt gccctttact gatattattt tatatccgcc
     8461 ttcataatca tatccatttg taatttctaa taatgatttt ccattcataa ccggctcgat
     8521 ggcgctattt ccggtagtaa atttatcgga gtctttttct ctgatgttct gatccaattg
     8581 aacccacagg tattttagaa tatcaggtga attattatga taggtgatgg tttcacttcc
     8641 tgttatcgat tgattaatat catttaattc aacttcgatc tcataatcag ctcgttgttg
     8701 ccagtaatct ttccctggtg ctcctgaagc agttcgataa gtgtttggag taggaagaat
     8761 tgtttcaagt tgctcaaact tcttcccata atcaccctga ctcatcccca aaagcggaaa
     8821 aaccattgat agtgcggtaa ataatttttt catctcacca aaatttattt tcaataatta
     8881 aaataaaagc aatgccggct atggcagaag aaattaccat tacccaatca cgtcttgaga
     8941 tataaaagat tccatttgca ataaatgcta caatcatata aatcccaact attattattt
     9001 gacctgcctc aatcccaatg ttaaaggcaa ataattgtgt aataaccgag ctgtctgttc
     9061 ccaggagact tttaagatag tttgaaaaac caaggccatg aatgaggcca aaaaataagg
     9121 caaaaaggta attgacctga atccttcgtg tgccggagga gggcttactg aaaatgttat
     9181 tggttgcagt aattaaaatg gtcacaggga taagaaactc tacaacgtcc gtcctcacag
     9241 aaataagttc aagcgttgca aggaccagag ttatagagtg accaattgtg aaagcagtca
     9301 ctaaaatcag aacccggctc cactctctgg cattgtaaat tacacaaagt gcaataatga
     9361 atagtatatg atcgtaccct cttgtatcaa gaatatgttc aactcccaat tgaaaatata
     9421 atccgaactc tgacatctat gttatttcaa atttcattct ccaaaacaat aaaggaaggc
     9481 aaattaaagt atttttgatg agaaatgatg ctaattattt tacttgcggt attggttcat
     9541 cccattcata tttcagtggt ggaagttcgg ttctccgaag attccaaaac ttttcaactc
     9601 agtcaaagaa tttttgttga tgatttggaa gctgccatta ataaggagaa ttctacttcc
     9661 cttgatattc tgaagctgga ggattcagtt cgtaataaaa ttctgagaag gtatcttttg
     9721 gccaaaatag aaatcagact tgataacaaa aaaactgtgc ttaattatat tggttctgaa
     9781 atggaagagg gtgtttgttg ggtatatttt gaatcagcca aaacaaaatc ttttaaacaa
     9841 attacactta aaaatgttgc tttatttgaa ttgtttgatg accaatccac aataattcaa
     9901 attgaaaaag ataaaacagt aaagagtttc agattgtttg gtgaaaaggt ggaagaaatt
     9961 attgtttttg agtgaggaag cgaatctcga aatatttttc ccaaattttt ccggtatgct
    10021 tcaacaggaa cacagaattg caagcgaatt gatcttcaaa atttttaaca ccaaactttt
    10081 cccatgcctt attgaattct tgtttcttca tatccctgaa agctaccgtt atgtgcggat
    10141 ggaatccctg gtttttataa tcctgattta gaaaattaaa ctcggttcgc atcaccctct
    10201 tgataatact gtgcaaattg gttaacgatt cactttccat aacctgtata aagataactt
    10261 tatgagggaa tgacccgtaa ttttttaact taacttcaaa tggtgagaaa ttaagtaaca
    10321 cttccacaag tcttaatttc aattttcccg ctttttccag ggacaattta aatggcatat
    10381 gcaaagtaat atgaggcggg gagcgcagcg ccgcctttga atcataatct ttggcaaagg
    10441 attcctttat attcattatc attgaataca atggctcctc cggaacaatg gcaataaaaa
    10501 acaaattttc cttcactgtt ttatccatca gaactaaact aaagtatttt aaaacatact
    10561 tatggaaaaa atcctgattt agtagttaag aaaattggaa ttcaaaactt aaacttcgtg
    10621 attataaatc cgccttagtt gggttttttt tgttaatttt catgaaaaga attcatatga
    10681 ttatagtcaa cacagaaaca attgccggaa gagagatcaa ggaaaacctt ggtattgccc
    10741 ggggttcaac agttcgcgcc cgtaacatcg gaagagatat attcgcagga ttgaaaaaca
    10801 tcgtaggcgg agagattacc gagtataccc agcttctggc ggacagccgg gaacaagctc
    10861 tctcaagaat gataaaagat gctgaagaaa tgggagctga tgccatagta aatgtacgaa
    10921 ttgcaacctc cgctgttatg caaggttctg cagaaattct ctgttacggc accgcggtga
    10981 aacttcgttg attttaacct gattaatatc caggtgaaat ccgaaatttc tactttttcg
    11041 ccctccggaa gatcattcca aggtcaaaaa tttaataaat caatcccggg taattcttcc
    11101 gcattggctg ttatcggggc cggtatttta ggagtttgca gcgcctatta tctgagaaag
    11161 gcgggattta atgttacact ttttgataag cgcggacccg gaaactctgc ctccagttcc
    11221 ggtggtgaat cacggttatt gagacttatt tacggggcaa gccaattcta ttcccggtta
    11281 actctggatg ccattccgct ttggatggaa ctccagaagg gaactgaccc ttttttcatt
    11341 ccaagcccgg tgctttggat atcaggtaag aaaaataaaa agataattct tgattcactc
    11401 ggatttcttg agagcaacaa gatcaggtat agagaattat ccagggaaga tcttcagaag
    11461 aaatggccct ttatgcaagt gtctgacatt gaaatcgcag ttctggaaga attatccgga
    11521 tatctgaaag cacgcaacgc gtgccaggcc attgcatcaa aatttgttga agaaggcgga
    11581 aatatcattt gggatgatgt taaacctgag aaagcaggca cggagcagct ttcaggttta
    11641 aaactttcaa ccggcggcgt tgagcatttt gattattttg tgatagcggc cggcccctgg
    11701 ctaaatcagt gggttgaatc tccggtaatt gaagtgacca ggcaggaagt acattttttt
    11761 gacgttccgg ccaattcagc ttccatgaaa tcttttactt cctgggttga tttatcctat
    11821 gagccgtatt tctacggtat ccctggtgac ttaacccgcg gattcaaaat tgcctgtgac
    11881 aaacggggtt cgctatttaa tcctgacctt gatagcaggg ttccggaaat tgatttacta
    11941 caagaaagca gaaaatttct gcgttttaga tttcctgaac tggctcagct acctcttctg
    12001 gaatcgcggg tttgccagta ttcaaattct tttgacagaa atctgataat ggacgtacat
    12061 ccttcttaca ataatgtctt gcttttgggc gctggttccg gtcatggttt taagtacggg
    12121 ccggccattg ggaaattggt ctctgaattc ataccagggc aaaaacctct ttttaatgag
    12181 atgcggcttt tttaaagtgt ggccagttca ggagtctcaa attcatttcc aatcactttg
    12241 ctgttatttg cctctgatct cccgaaggta tgtgaaatca tttttcgctc cacgtcggtg
    12301 taagaccaac ccccgcgatt attatcccaa attacaataa accttttacc ctcaatctca
    12361 acgatatcac gctggtacac ttccctgttg ttcatgtctt tttgacctgt gaattggagt
    12421 atttctatat catcatcttt aaacaacccc atctctttat cagcaaaaac aacaaaagaa
    12481 tgccgctcac ttgaaaaaga taatgacccc gaaatgtaca ttttcttttt caccttattc
    12541 caaaccttaa attttaactc attgttgagc atacaggaaa ttttattcga taagttaaca
    12601 attttcaatt aaggattcac tttcaaatct cgtttcatat aaaaaataaa attacgaaga
    12661 gcatcgttaa ttgctacatc agcatcccaa acagaaccta tgaatatttc cccagtaact
    12721 gtattcctga aataaaattt gaaaaccatg cgatcagctg gaatgcttcg aattattggt
    12781 ttttctgaca agactgcatt tgttatgatt tctttagaat ctccggagct caatttaaac
    12841 atctgggtta tatcatttgc tctaccataa acataaccca gaaagaaatt gcatcctttg
    12901 gttgttctca attcttccat tgtaaggcca aagtcgtaga attcatattt aaatggatat
    12961 ttttcagtta taacattttt gagctcatca tttctggcca taatagcatc ctgatacgtt
    13021 tcaaaaactc tctgcaggtt tacatttatt tcttccggtt tggaggttga gggtattaat
    13081 gctataccgg ttttaaaacc ccggacatca ggtgggtaag ccttgcttat ttttctttta
    13141 tgaaagatta gcccttcata atattccggc acatctgaaa tcagcagatt ttttcgttcc
    13201 tgctccagtg ccgccttctg atatagtggc aacaaagctt tggaaacagt gctgtcgctg
    13261 aaataccagg cattttgacc gctggaaatc actgaccagc tgttgttaaa tttagtcagc
    13321 cagacctcgg ctttgacttt tgtcgcccta accaaaatta tgttcgttac ttgtctcgtt
    13381 ttcaggaatt ctgaaatttc ctgttgtggt tcaaaccccg aaaacacggt tttccattgg
    13441 aaatacgcca ccgcatcaat tccagttctt cttagctgga tttgggtata attcgaaatt
    13501 tctttccaat tcaatcccga actcccaacc tcaacgataa cggcagtttt tgtagatagt
    13561 aaatcctctg gaatttcaaa ggagacattg agatacactg gagaatttgt caacagtata
    13621 tcctgtgcga tgagaggaga acttatgtag accaggagta tatatatata taatagtttc
    13681 cgcatacatt tgtggataag tctttaattt agcagatcat atccataaaa agggatgaca
    13741 aatttaataa aatccttact tagcatagtg gtattccttg tattcccttt ttccacttcg
    13801 gcccaaattg atcaagttgc tctaatgaat gatctcagga ttttatctca cgattccatg
    13861 gaaggaagaa aaaccggcac tgatggaaac gaaaaagcca gagaatttat tttgaaaaga
    13921 ctgagagaaa ttgccgtaaa accactggaa tcagattttc agcaaaaatt taacttctct
    13981 gccagtgatg gagaaaaaac agggacaaat attcttggtc acattgaagg aattgcttct
    14041 tcgaattcct atttcgtcat cagcgcacat tatgatcata tcggtgttag atcaggagaa
    14101 atttataatg gtgccgatga caatgcatcc ggagtagctg cattactcgc gattgccaat
    14161 tatttcaaga aaaattcacc acgacacagt atgatttttg cattctttga tgcggaagaa
    14221 tcgggcctgc agggggcaaa agcttttgtt gctacaaatt ccagggcaat tattctcaat
    14281 gtgaatatgg acatgatttc tcgtaatgac aagaatgaaa tctatatcgc gggaacctat
    14341 caaaatcctg acctgaagga gttagtacaa aaatcgcttt cccaaaacga ccggttgaaa
    14401 gtactattcg gccatgatac tccaggttct ggtggacatg actggtctaa ttcatctgat
    14461 cacggaccat ttagcaaatc ggggattcct tttctatatt tcggagttga agatcatgaa
    14521 gattatcata aacccggtga tgactttgaa aaaatagata ttaacttcta taattcagtg
    14581 gtagaaatgt tagttcatgt tgtcaattcc ttagacaagg aactcgaata atactttttt
    14641 tgtgaagaga aaatttcaag tagcaataat tggaggcggg cttggtggac ttatctgcgc
    14701 aaattatctg gccagggaaa agattgaagt ttgccttatt gaaagaaagt catatccttt
    14761 tcaccgggtt tgtggtgagt atgtttccaa tgaagttaaa ccctttctgc agtcccttga
    14821 attgtttccc actcatttta atccaccgga aataaatcga tttattttaa cttccattaa
    14881 tggcaagaaa gccgaaacag atttacgctt aggaggcttt ggtatttccc gattttcttt
    14941 tgaccacctt ctatcagaaa aagcaaaagt gaatggagcc gttattctta cgaatagatt
    15001 tgttgatgat atcgcctttg actcggggac tttcaaagta tcaattagcg gtggcaaagg
    15061 cattgaatct gatctggtga ttggtgcatt cggcaaaagg tcgtcattgg ataaaaaact
    15121 tagccggcct ttcttaaaac acaagtcccc gtacattggc gtgaaatatc atattcatta
    15181 tgaactgccc aaaaatgaaa ttcaactcca taattttgaa ggtggatatt gcggaataag
    15241 taatgtggag aataataccg tcaatctttg ttatttaagt gaacgtggta atcttaaaaa
    15301 atgcggcaat attcaaagta tggaggagaa agttttgcag aaaaaccctt ttctaaaaga
    15361 aatatggaga aatgctgaat ttgttatgaa taatccgata gttattaatg aaatcaattt
    15421 cgaatcaaaa aatcaggttg aatctcacat attaatggtt ggtgactccg ccggaatgat
    15481 cgcgccttta tgcggaaatg gcatgtcgat ggcgattcat gcagctaaaa tagcctgtga
    15541 agaaatcgtc cggttctttg gttctcctga aattactatg accagattgg agacgaatta
    15601 catggaaaga tggaaaaacc aattttcttc ccgaatggtt ataggtcgta atttacaaaa
    15661 gctttttggc agcgtttggt taagtaatgc ctctgtcgga ctattaaata attttggaac
    15721 actgggaaaa ttcctgataa ataagacgca tggtcaacct ttctagatca ctatttgatg
    15781 aattttttca gggatcttga attatttgaa cttatctcga atagatacac tcctctcgtt
    15841 aaatttgaga tttcactttt aaaaatgaat tctcctttac cggcattttc cttccaggat
    15901 ttcacaattc tctcacatca gaattaaatc gaaagaaata tcttacatgt caaaaaaagt
    15961 tattaacagg aagaaaagac tcaacccaat tgaagaaatg aggttaagag tcatggtgtt
    16021 tcgataatcc gctttttgtg gatttattct tacctgaaaa taccatacac caaaaaaaat
    16081 tactgttggc agcatgcatc ctaaaaaaag agcggcagtt attgcggaat gattctttag
    16141 aaaaaataaa aagaagcaaa gcgtcgctat tccaaaaagt attcctgtaa aatgaaatgt
    16201 gcccaataag cccagcttat aacttatagt gatatctccg cgtttttcat cctcttcatg
    16261 ctgataaacc tgggtcatag gataggaccc taacaataga actgtggcca atactgctgg
    16321 ctgccatccg tcagaaatgg tttcctgaat tggaaggtca ttcattccct ggaatgagat
    16381 aagataggtt ataaatccct ggaatgtccc tatggtcaac caccctataa ctggatacct
    16441 ttttagccgg ataaatggat gactgtaggc tttcgaagct aatccataaa tcaatattcc
    16501 tgttccaaag atccagctaa taaaaaaagc gattattaat gctactgcat ccattactaa
    16561 ggccgccaga tataattgcc tggtaacttt agggggagtc tttattcccc cgatgctgcc
    16621 ttcatcttta tcaaaataac tattgtaccc gtttgaagca ggatacacca acaaatgaag
    16681 gacaataaaa ataagaatag cattttcata agaaacttca ggtgattgtg ataaggcaaa
    16741 taggtaaatt ggcaataaga agacggaaaa agggaagcga agatgaatga tggttgaata
    16801 tttgatcatt gtagtctaaa tgaaccaaat tataactttt ttgattttac cactatggga
    16861 agctatatta aggcaatagg aactgcaaat ccgggccagc cgataccgca gaaaagcatt
    16921 gcttccttta tggttaaagc ccatggcctg gaagggcagg aacaatccaa acttgaggca
    16981 ttatttagat ctaccggaat ttccagccgg tattctgtta tttcagatta ttccgggaat
    17041 ggtcaacgta atttcttccc aaaaaccaat aatctggagc cattccccag cactaaaatg
    17101 cgaatggacc tttttcgcaa taaagctgtt gacctttcat taagagccgt atatgattgc
    17161 cttggtaata aatttcaact taatcagatt acgcacctca tcacagtaag ctgcaccgga
    17221 atgtatgcac caggcctgga tattgacctg gttacccaat tagggttacc cagtactaca
    17281 tcacgaagtg caataaacta tatgggatgt tatgccgcta ttaatgcctt gagaatcgca
    17341 gactccattt gcaaatcaga tgtcgctgcc aaagtgctgg tagtttgtgt cgaattgtgt
    17401 tcaatacact ttcaaaaagt gaattctgaa gataattatc tggcaaattc aatctttgga
    17461 gatggggctg ctgccgtatt gatatcatca ggcatgacta aagaaatgga tttggagctt
    17521 ttaagttttt atagtgacat tctggattat ggcaaggaag aaatggcctg gacaatagat
    17581 gattttggtt ttcagatgaa gctttcttcc tatgtccccg acgtcatcag gaaaggaatt
    17641 gctgagctga cgtataggtt tctttccggc aataatctgg aactaaagga cattgatttt
    17701 tttgcgattc atcctggcgg gaaaaggatc ctggatgaaa ttgaatcaga actgggaatg
    17761 gaaaaatcca agaatcaata cgcccgggaa gtgcttaaaa attatgggaa catgtcttcc
    17821 ccgacagtgc tgtttgtagt caaatcattg ctatcaataa taaaaaagaa cgataaaggg
    17881 aaaatcatcc tagtaatggc ttttggacct ggcttaacta ttgagacagt gttgttaagg
    17941 gtggtttaaa tccctcattt tgggtgatat actcaattat gtgggtaatg agggcaaaaa
    18001 aatcattcaa aatcctttac tcgtataggt ctaatttcta catttggtgt tctttcgaat
    18061 taatttttaa tcataaacct aagattgaca atatgatata gacttctacg ttaactaaat
    18121 gcgatcatta aatgagtaat ttaagattga gtgacagcga attaatatcg ctatacaaaa
    18181 atggtaacga agaggctttt gaatcgattg ttaatcggca caagcgaaag gtttatacct
    18241 ccatttatct tattgttaaa gacagttatg tggcagagga tctgttgcag gaaacattta
    18301 ttaaggtggt gaaaactgtt aagtccggcc ggtataatga ggagggtaaa tttttaccct
    18361 ggttaatgag aattgctcat aacctggcgg ttgatttctt caggaaagaa aagcgttatc
    18421 caaccataat catggaagat ggagataaga ttttcaataa tctacagttt tccgaagact
    18481 catttgagtc aaagcaattg gttacagata cgcataataa attaaagttt atgattaagg
    18541 aacttcccga gactcagcga gaagttctgg taatgagaca ttacatggag atgagttttc
    18601 aggaaattgc cgaagcaacg ggcgtcagta taaatacggc tttagggaga atgcggtatg
    18661 ctttgattaa cctacgcaaa aaagtaagaa aatataacat tgcctatgac aaaaacattt
    18721 accccgaatg aattaatcag gtattattac aaagaaactt ctgaaaaaga aaacagaaaa
    18781 attctaaatg ctttgatttg tgaccctgtg ctgcaggagc agtacaaaga aatttccaag
    18841 gtcatcaggc aacttgacga tgttaagttc aacccctctg acaaagttat taaagccatt
    18901 ttggattact caagaaatgc ggatgtgcat tctttaaata agtaatgctg cgaaaggaaa
    18961 ggtatgaagc ctttgtaagg cactttacca aacatcaacc tgaggcaacc accgagctta
    19021 attacaccaa tccctatgag cttttggtag ctgttatttt aagtgcccaa tgtacagaca
    19081 aaagggtcaa cttagttact ccttcactat tcaagaagta ccctactcca aaacatctgg
    19141 cagtcagcgg ctttgatgaa ctattccctc tcgttaaatc catttcttac ccaaacaata
    19201 aaacccggca ccttattggg atggcaaaac ttttggttga gaaatttaat gggcgggttc
    19261 ccgtggagct aaaacatttg caggaacttc ccggagtggg gcggaaaacc gcaaatgtga
    19321 ttgcttccgt gatttacgat cagccattca tgccggttga tacacatgta ttccgtgttt
    19381 ctaaaaggct tgggcttgtt cgtcaaaatt caaaaacacc gcttgatgtg gagaaacaat
    19441 taatgaaatg ccttcccaac gatgtaattt cagtagccca ccattggctg attctgcatg
    19501 gaagatatgt gtgcctggcg aggaaaccta aatgcaacct gtgcaacatt tctcattttt
    19561 gccgatattt tgagaaatta aaaacatctt gaaaattctg attattcacc agcattttcg
    19621 caccccgaat cagggtggat ccattcgttc ctatcatata gccagagaat taagtctatt
    19681 aggtaaccaa gtgagtgtta tctcttcttc acaaaaggca aggaattatg taaccaaggt
    19741 tgagaatttt gaaattcgat atttgaaagt aagatatgaa aacagttttg gcttcgcccg
    19801 gaggatgatc tcctattcca gcttccttct taaagcaatc tattcgctga agttgtacaa
    19861 ggattatgat gtttgttatg tgatgtcgac tcctttaagt gttgggatta tcggtttgtg
    19921 ggcaaaaagt cattacaaaa ttccttatgt ttttgaagtg ggtgatctct ggccggatat
    19981 tcctattcag atgggaatta tcaggaatcc gattttcaaa tggagtgtgt taaaactgga
    20041 gaaactcatc tattcgaaat caaaattcat tgtcgctctg tcaaatgaaa tgaaacaatt
    20101 gattcaggtt aaagtaaagg ataaacaaat tacagtaatt gaaaattttg ctgattccgc
    20161 atttttcaaa ataaagaatg tggagattaa aattccttca actattaaaa aggggaatgt
    20221 tgttatttcc tatataggaa cattaggaat tgcaaataag gttgaaaccc ttatcaagct
    20281 ggccggaatt ctaagggaag aagcgattac gattgtaatc aaaggaagtg gtaaggagtt
    20341 gcaactttgc cagggtctgg cgaataaatt aaatctgcac aatgtaattt ttatgccatt
    20401 tggttctaaa gatgaaatta aagaattgct gtcatatact gatgccatct atgtaagctt
    20461 tgataagcgc tatcctttgc taaccaccgg ttgcccgaat aaactcatcg atggcattgc
    20521 tgctggtaac attgtgctta tgaactacgg tggctggcaa aaagaattga ttaaaaatac
    20581 taaatgcgga ataattatgg acattgacaa tcccgctgat tgcctgaata aagccaggtc
    20641 aattctcctt gaacctggga aacgagaagt aatgaaaaat aattccagaa tgctggcaca
    20701 aaaatttgaa gtaaagttac tggtagaaag actgtattta gaaattgcaa aagctcttaa
    20761 ctaacgactt ggcttgtagc cggaattgtt aaagattgct gttgcacttt ggatttccat
    20821 catttgatgc acgctgatat cggggttttt ttcacggtaa cttcgaatta tttgccaacc
    20881 cagccaggct ccgattctac ccggacattt atccccgatc tcaaaaacag tcggccgttc
    20941 atccatatat ttcgtttttg tcatgtgatt agtttcaaaa attaattggt ttgccacaaa
    21001 attggcccaa attatgtctt cattttcatt tacatctgtc aattgctgac ccgaatacca
    21061 gataatcagg ctatctggag cacatggcat gattgatttg gcaaactcgt atttcttccc
    21121 ataataaacc atgtcagcca gcattgtcct gtcactgtaa tcatccttaa caaatttgtt
    21181 agcaagaagt aaaataatgg cgggcactat atattctttt tgatagcgtt tgagaatata
    21241 gttaggaatt tcaatcggtc gatacgttgc atatggaccg atgtaataat cgagcccgat
    21301 aataatcaaa gaatcgctta cataaagttc gctgcttcca aaccctgtaa ccatggtctg
    21361 aacttttgga attaagattt ccggataaaa ggatttcagg tatctgtatg ctgcttccaa
    21421 ttctaatttt aaatcgctaa agtcagcaaa ttcctgccga acttccctca gtagtgtatc
    21481 aatgtgagga tctttaatgc ggcgaaaaag attattgatt aagatagaat catcaggata
    21541 ttgatcgcta gccaaaaaat gaattttcat tgcagaattt tcgttgagga aatcccgcac
    21601 ttcctcagaa ctttgaaggg aaaacattgt ttcttcaagt cttgagattt caatgttgac
    21661 cgtgattccg gaaattgatt catcaacctc acaatcatca ttcgcacaag attgaagaaa
    21721 ggtacatata acaagaaata agctgccctt gtatatcata aggacaaatc tagtccatga
    21781 aaattagcaa taccaaacca tgaattataa tttaacgttg aattcactat tggtaatgaa
    21841 gttgaggctg attatatcat tgttcattta ttctcatttg ggaatttgtc agtcggtatt
    21901 agatagcctc acgcggtcag aggaaagaaa ttataacaag atttcatacc aatctctgcg
    21961 ggtgccttcc tccatttcta ctaatgtttc tctcctggca gattatctta ctcaagaggc
    22021 aaaaactgaa cttgaaaagg tgcgggcatt ctatgtgtgg attacttcta atattgccta
    22081 cgattttcgg tctcttgaaa aaaatttata tcctgttcaa actccggaag ttgttattgc
    22141 aaggagaact gccctgtgtc agggatttgc agatttattt catgaattat gtgaagaggt
    22201 agatataaga tccgcggtga tttccggata ctctaaagga tatggttaca aacctggaaa
    22261 ggaattcttt gttagtgatc atgcatggaa tgcggtaaga gtgaacaacc tctggtattt
    22321 gttagatgca acctggggtg caggtagtgg aattactcag aagtcgggtt tggaggatgg
    22381 ttttaatgat aaatattttt taactccgcc tgaaaagttt gttgaagatc atttgcctct
    22441 tgatcccatg tggcaattat taaacaatct tgttcctctt caagtttatg aaaagggaga
    22501 agctgaaata aatgctttta tggaaacagc acataaaact ttcaacttta aggactcatt
    22561 aatgagatgg gaaagtagtg atgtgtatca ggtagccatc gaatcacata agcgaagtat
    22621 cacctttaat cctaaaaata aaagagcggt ttacgaattg ggaactgaat tattgttcaa
    22681 ggcgctggat gtaatggatg gtattcatgg tgtttcttac ggagatttcg acaatcttgt
    22741 tgaggaaatg cgaaaatcaa tgtttgattt gcttcatgaa gctgcgtttc atttttcaaa
    22801 cattccatct tctcatcccg attatgagac agcatccaca ctattagaag aggtgacatt
    22861 tcaaaagggc gtcttttatt atgaagtggg tcaaaggatt tatgacattt tcttagaaat
    22921 ggatagggag gccttttata gaaatcttgc aaacgggatc ttgcaaattg ataattatta
    22981 tgatctggct acctattact tcaaacttgt taatgaagag agtcgctact ttccccaggc
    23041 ccgggaatat atacaaaatt atataataaa taaaaatcca ttgagaccag gtaattagat
    23101 ctcaattact tccgcttctt tattcaggaa tttgaaatcc gtaataccta gcgaagaatc
    23161 ctctatcaaa gtgatccatt tcctgtattt aaagaaccac ttcatttgcc tggagatcac
    23221 tccattggta aagtaagcat ggatgtaagg atgtacggca atagaaattg caggctcatt
    23281 ttgtttaata atcaaatgat ccaggcttgt ttcaataata tctgatacaa gaattgaagc
    23341 tttaatcttg cctgttccgt tacaggttgg acaggtctcc agcgtggtga tattcatttc
    23401 cggacgaaca cgctgccgcg taatttgcat aagaccaaat ttcgacaaag ggagcactgt
    23461 aaattttgat ctatcagcgc tcatttcttc cttcatcttt ttctgaagga gttttttatt
    23521 gtcagatttt cgcatatcaa tgaaatcaat tacgatgatt ccacccatgt cgcgtaagcg
    23581 aagctgccgg gcgatttccg ccgcagcttc caggtttact gataatgcgg ttgcttcttg
    23641 atctacttcc ctgtttgact tgttgccgga gtttacatca ataacatgga gagcttcggt
    23701 gtgttcaatt attagatagc ctccagcctt caaactaatt gtttgtccga agagcgattt
    23761 gaactgcttt tcaatgccaa attgttcaaa gatcttggcc ttgccggaat attgttttac
    23821 aatcttttct ttacctggag caatccgttt gatatatcct ttaacttcat catagatttt
    23881 tggatcgtcg acaaggatat tgtcaaaact ttcattcagg acatcccgta ggattgagga
    23941 tgctttgctc atctcaccaa ttactttttc cctgggcttt gcattgcgaa gtttcttaat
    24001 accttctttc caggtattaa ccaggttgcg aagatcccga tccagttccg ctacatcttt
    24061 acccagggct gcagttcgaa tgatgactcc gaaattctcg ggcttaattg aggtaaccaa
    24121 acgggtgagc cgtttacgct catcagcact ggaaattttt ctggatatgc ttactacctt
    24181 agaaaatggt accaatatca gataccttcc cgccatagat aactcgcagg atagtcgagg
    24241 ccctttcgtg cttatgggtt cttttacaac ctgaaccaga attttttgat tcctggttaa
    24301 aacttggtta atcttaccaa atttgttaat gtcaggttca ttcctaaagt tcccaaggtc
    24361 acccgaaatg tttttgttag ataaaacgag cttagtaaat tttgataagg aggcaaattg
    24421 cggtcccaaa tccaagtagt gtaagaaggc gtctttttca tggccgatat caataaaagc
    24481 tgcatttaaa ccttgtacaa ctttcttaac agttcccagg taaagatctc caacattaaa
    24541 ctgagttcca tcttcatcta tatgaaactc tactaaacct ttatctttta aaagggctat
    24601 tcgacatcca ttttgagtag aattaattat tagttcgcta ctcatatgat ttgtcgttcc
    24661 ctaagaaaaa gttattactt cttcttatgt ctgttctttc gcaatcgctt ttttcttttg
    24721 tgcgttgcta ttttatgtct ttttcttttt ttaccgcaag gcataaatct ttctgcgttt
    24781 taattttcaa tatcctctaa atacgaatcc acgtttgcca aaacttcagg atccttttct
    24841 aattctttta ctttcaatag atgttctttt gccagttgct tctggtccag ttcaagatag
    24901 ctaagtccca ggtagaaatg cgctctggaa ttctcgggat ttaccgcaat tagttttgta
    24961 aacctttcca ccgctctttc ccattgtgag gattgcatgg ataaaattcc catattgtat
    25021 aatgccattt cattattagg gtcaacttcc aaaacttccc tcaaaactat aatcccttgc
    25081 atcggattac tggaggcgat atacgttaaa ccaattttcg tcttcacctc caaatcctgg
    25141 ggattctgaa ctaacacctt atcaaagaac attcttgcct gctgacctag catttcgatt
    25201 tcattctgac ttattgcaaa gccctgggcc tgaaaataag cctccccggc cttttctaag
    25261 ttgtccgggg tttcaaagcg ttccgcttgt ctccccaggt aataggccgc gctgtcccac
    25321 tgattcatag cgcgaaatgc ttccgccaac gagtcggcaa agatagagct tttttcgata
    25381 ttatcagtct tttcaagctg agttttgaag tccaaaaccc gctgatgttc ctcaggagag
    25441 gcttttgatt ggtgaaatcc tgtgccttgc tgagcagatt caggcgttat ctcttcaggc
    25501 ttattatcta caactacttt tgggagtgag tagaggatag caatcaaggc tattcctacc
    25561 agaagcagta ttactctgtt tttccacatt tgagtatctc atttaggaga aactacttgc
    25621 tgtttacaag ttttacctta tcgcttttct taattttttc tacaaaaacc ttggaaggct
    25681 tgaaactggg aatgtagtgt tcatcaataa caatagcagt atttttggag atattccttg
    25741 cgatcttctt tgctcttctc ttattaataa aacttccgaa cccccttacg tatatgttgt
    25801 tcccgtcaga catcgcattc tttatcacat taaaaaatgc ttccacggtg attgtaacat
    25861 ctgctttatc tatcccggtc ttttctgcta tttcggtaat tacatcggct ttggtcactt
    25921 ggaaaatatt tagatttaat taaaacttct ttttacaatt gttattgata attctaacaa
    25981 gggtcacaaa tttatgttag caaaattttt aataaaagaa agagcatcaa aataattcat
    26041 cataaatgaa agtattatga aatataagcc cctgagcgtt aattattttg ccaggcgggt
    26101 tcttcaatgg tactaccggg caaaaaggga tttgccctgg cgaaatgaaa gggatgctta
    26161 tacggtttgg gttgctgaaa ttatacttca acaaacaagg gttgagcagg gtttaccata
    26221 tttcaaccgg tttattgaga cctttcctga tgttttctcc ctggcaaaag cttctgagca
    26281 aagtgttcta aagatttggg agggattggg ttattatagt cgtgcaagaa acatgcataa
    26341 atgtgctcaa ataattgtga ataataatca tggaagtttt ccaaagcggt ttgaagatct
    26401 cttgcagctt ccggggattg gtaagtatac agctgctgca attgcatctt ttgcttacca
    26461 tgaggcagtt ccggctattg atgcaaacgt aattcgggtt ttaaccagga ttttcaggat
    26521 tcatgaagat cctcataaac ctaaaactca aaaactctta tttgagaaat cccttaaact
    26581 aatttcgaag gataatccgg accactacaa ccaggccatg atggaacttg gagcacttat
    26641 ttgtaagcct aaaaaccctt cctgtgcccg gtgtcctgtt atggattgct gtgaagcata
    26701 tgcttcgggc gatcaacaga aatatcccta taaaaaagca aggggtaaga tccgcaaaag
    26761 gtactttaat tatgtaattt taaaatcggg agataaaact tattttcagt tgagaaatgg
    26821 ggatgatatc tggaaaggtt tatatgaatt ctttcttatc gaaagtaaag cacccattga
    26881 catttctgat attttgcaca aggttccaat tgaacttctg gaggagccaa gagtgttata
    26941 cgaaagcaag gagtataaac atcagctaag tcatcaagaa ataagagccc ggtttattga
    27001 aattagttct ccaggtattc aaaccaaaca atggttgaag ccttttctct tcaaaaaaaa
    27061 gatggtcaaa gaccttccga agtcagtttt attaaataaa tatttggaag aaagaccaat
    27121 ttaactatat tgagattttg tttaactatt taaatattaa ttatggctgg agtaaataaa
    27181 gtaattctgg taggaaacct gggaaaagat cccgaagtga ggcaccttga aggaggcgct
    27241 gctgttgcaa atttttcgtt agctacgtct gaatcgtaca aggacaagac cagcggagaa
    27301 cgcaaaacta ttaccgagtg gcacaatatt gttctttggc gcgggctcgc tgaggttgct
    27361 gaaaagtatc tcaaaaaggg cgattcggtc tacattgaag gcaaaataag gtccaggcag
    27421 tgggaagaca aggaaggcaa aacgcggtat acgaccgaaa ttgttggaga caacatggtg
    27481 atgctcggaa gtagaggtgg ttcaagttca gggtcacaat ccgctgaaaa ggacattgtc
    27541 accagcgatg ccgatgagac ggatgatctg cctttctaag gacttccgga ctatgtttta
    27601 ttaagagcat gaacgaattc ttttgtaaat ttgcattcgc gaaaaattga tgtgaatcag
    27661 aaaattcggt ttatgttgaa ctaaaccccc tttttttgga agcaagtctt gaagaacctc
    27721 cgagtttatt tttattacaa ataattacac cattagttat cggttacggt gtgagcatca
    27781 ttcttttact tatcctttca gcattaattt ccggatcaga ggtagcattt ttttcccttt
    27841 ccaatcagga tataattgat atgcgaaaga atccgaccga atcaggtaag cgaacaatgg
    27901 tccttttaca gaatccaaaa cgacttcttg ctaccatttt aatcctgaat aatttagtga
    27961 acattgccat tgttacttta tcaacatttt taacatggga attagtcgga actcattcac
    28021 cggagggagt tatccttatt attcttacct cctcggttac ttttgctatt gttttttttg
    28081 gagaagtaat gccgaaggtt tttgcagttc agaattatct tctttttgca agaattactg
    28141 ctcctttact ttggatttca acgcgcgttc taaagcccgt tgcctgggtg ttaatgaatc
    28201 tttccggtgt tatagagaag aggtttgaaa agaaagggta ttcgatttcc gtagacgaac
    28261 ttcatcaggc tctggagata gcaaccgata agaatacctc tgatgaagaa aaagaaattc
    28321 ttaaagggat agtcaacttc ggcacattaa ccgtgaaaca aataatgaga tcacgtcttg
    28381 atattaccgc cgttgatgag gattgtaatt ttcatgagtt acttgacaaa ataaacaaat
    28441 ccggatactc aagaatacct gtgtttaatg agactattga cagtatctcc ggcgttttgt
    28501 atataaagga tttgctgcct tacattgaag aagaggagaa ctttgaatgg caaaaattgc
    28561 tgcgtccggg aatttttgtt ccggagacaa agaaaattga tagcctgctg aaggatttcc
    28621 aggaaaaaag agttcacatg gcaattgtag ttgatgaata tggcggaacc agcggtcttg
    28681 taaccctgga agatataatt gaggaaataa taggggaaat aaatgatgag tttgacgaag
    28741 aagattttat ttttcataaa ctggatgaac aaacttttgt ttttgaaggg aaaacttctc
    28801 ttaatgattt ttgcaaagcc atagatgttg atgtgtctgt ttttgatgat ataaaaggcg
    28861 aaagtgaatc tttgggagga ttaattctgg aaataaattc aactttgcct caagccggtg
    28921 aaaagatata ttttgacaat tttgttttca ctatcgttgc ggttgatcaa aagagaataa
    28981 aaagagttcg agtattcatt aaagaaaaag cccgtgaagg aaaatcaacg gagttcactg
    29041 actaattcat gggcaatact tcttttctca tttgtactac ttgcggctgt tggttcctgc
    29101 acaaaagatt ggcttccaaa accaagaggg tataaccgac ttcccttgcc tgagcatgaa
    29161 tacagattac tccctgattc atttccatat atttttgaat attccgctca cgcgagaatc
    29221 tataatgact cctcatggat tgcagaacct tattggattg acctttttta tccagacctg
    29281 atagccaatg ttcaaataac ttacaaacca gtgaacggaa atcaaaaact ccttgaagag
    29341 tatttatctg atgcgtataa gttgacttca aaacatcaaa taaaagccta ctctattgat
    29401 gaaagttatc tcataacccc gaccggaaaa accgctgttc tagctgaatt aggtggcgaa
    29461 gtacccagtc agtttcagtt ttatgtaaca gattctgttg aacattttct tcggggagcc
    29521 ttgtatttca gaacctcttt aaagaacgat tctctgtcac ctgcaattga atatgtgaaa
    29581 attgatataa ttcacatgct gaacacgctg caatggagga cggaggttcc caaaaatgtt
    29641 tctctccgcc aataagatat ggctaacttt tttgataccg gaatcgagtt tattaaagga
    29701 gtgggtcctc aaaaagcatc tcttctcgaa aaagaactta acattcgaac ctttggcgac
    29761 cttctgactc attatccatt caggtatgaa gacagaaccc ggttttattc catcaaagac
    29821 ttaaatgaat cattacccta tgttcaaatt aagggaactc taaaggcatt gtataccctt
    29881 ggcggtggga aaagaaaaag attggttgga tacatcgttg acgaatccgg tgagattgaa
    29941 cttgtttggt tcaaaggaat aaattgggta acaaagaaat tgaagatcgg gctggaatat
    30001 gttgtatttg gaaagcctac tctctttgca aaaaagttaa acattgcaca tcctgagatt
    30061 gaagtactca cggagacgag ttcaaaaatc agttacctgc aacctgtgta tcccacaaca
    30121 gaaaaactta aatccaaatt tctcgacagc aaggcaattt ctaaaattca aatcacatta
    30181 ctgaaagctg ctctgccgaa aatgggtgaa tcactccctt ctggcttgct taatcattac
    30241 gggctgatgc ccaagaaaaa ggcgttgcga catattcatt ttccttcaaa tcagaatatg
    30301 ttgtcaaaag cattgtacag gttgaagttt gaggaattat tttttattca gatgaagtta
    30361 ttgatgatga aggtttcaag aatggaaaaa tttaaaggtc atgctttcaa ttcagcagaa
    30421 ttggttacgg agttctatga aaaacattta ccatttgaac taacccgcgc acagaaacga
    30481 gtggtcaaag aagcttacag ggatatgcgt tccggtcacc aaatgaacag gctgcttcag
    30541 ggcgatgtgg gaagtggaaa aacaattgtt gcctttcttt gcatgttgct tgcaattaat
    30601 ggtggtactc aaactgcact aatggctcca actgaaattc ttgccaatca gcattataaa
    30661 ggattgagga cttttgccaa gaaaatgaac cttcgcatcg cattgttaac cggcgctaca
    30721 aatacaaaag atcgtaaagt tatccatcaa tatcttgcca atggagactt gcacatcctg
    30781 gtgggcaccc atgcgctgtt agaagacgtg gttcagttta aaaatcttgg attagctgtg
    30841 attgatgaac agcaccggtt tggtgttgcc caaagatcca ggctttggga aaaaaatgct
    30901 gtattaattc cgcatgtctt ggtaatgaca gctacgccaa ttccaagaac attagcgatg
    30961 gccttatatg gagatctgga tatttccgta attgacgaac ttccggccgg ccgcaagcca
    31021 attatcaccc ggcatcagta tgattcaaac cggttagaat tatttggatt tatcagaaaa
    31081 caaattggtg agggaagaca agtatacata gtttatccct taatagaaga gtcggaaaaa
    31141 tccgacctga aagatttgat ggatggctat gaaagtgtcc gtcgggcatt tcctgaattt
    31201 caggtaagca tattgtacgg taaaatgaaa agtgatgcaa aggattttga aatgtcccgg
    31261 tttcaaaaac gagaaactca gatcatggtc gccaccactg ttattgaggt gggggtggat
    31321 atacccaatg catctgttat gattattgaa aatgccgaaa gatttggact tgcccagtta
    31381 catcagctcc gtggaagagt aggtcgtgga gctgaccagt cgtattgcat acttatgacc
    31441 aggtatgagt tgggccagga tgcacgcacc cgaattgata ccatggttag aaccaatgat
    31501 ggttttgaaa ttgcagatgt tgatcttcaa ttgagaggtc ccggagattt gctgggtacg
    31561 cagcaaagtg gattgctcga tcttacggtt gccgatttgt caaaagatgg aaagattttg
    31621 caagttgccc gggaagctgc aattgccatt ttgaaaaaag acccggagtt aaaatccgga
    31681 gagaacgaat acctcaacca aaaactaaaa tccatctctg agacctttgt gaattggagc
    31741 agggttagct aagtttgata ttggactttc atcatatgaa aaaaattttc ttattctttg
    31801 caggagtttt attttcaatc tcatacctgc ctgctcaaca tgggcatttt tatttaacca
    31861 accatgtgcc caggacagtt acatttgaaa acaccaattt taaaataatc caaaatagtc
    31921 agggaatttt atatatagcc aataacaggg gattgattgc atatgatggc acgaactggg
    31981 attttataga aacaccggaa gccatttttt ctatcgccat cgatcagacg gatcagatat
    32041 ttataggtgg tcaaagcgga ttcggaaggc taataaaggt tagtcgaaat gagtttctgt
    32101 atgaatcgtt gtcggatagc gtagtttccg ccaatgaaat ctttgaaata aaagagcatc
    32161 ttggaaaaat atattttctt aaccagaaag cagtattcat ttatgagagc ggcaagatta
    32221 gcgaattacg attaaaagat gcatccgttt ttcggacttt cgcacctcta ccacaggagt
    32281 tgcttgccat taccaatgat gggagagtgt taagcatatc ggaaagtctt aaaagttttg
    32341 atccaggaat tgggtatgat gtaaccctgg aaacttcttt tctaagtcct gataaatcaa
    32401 aagcaatact aatcgattct gatggcaatt tgtttctata tgaaaacaga accgcagcta
    32461 aaatagtttt taatgataag gagttgttgt caaaatttat tgttacttct ggtgtttggc
    32521 tggaggggga tattcttgca attggcagcc ttgagagtgg agtagtcttt cttgatttaa
    32581 ataagaaaac cttgcttgag gtattgaact acgaatcagg gttgcctgat aatcaaatat
    32641 tctcgatgac ccgcgacaga gttgagaata gtctttggat ttctcacgca tatggattca
    32701 gccgcgtttc gccttttctt cctttccaca gctatgggag atttcctgga ataaacggaa
    32761 aacttttgac cggctatttt ggagggaacc agatttattt gggcacttca gtaggattat
    32821 tccggctgca ggaagtgcgc gagttcaggg aaaaagtgat ttacgttccg gtaagaacat
    32881 ctacaggacc tacatctgtt caggaagcag agaaactgca agatgaaaaa aagtcacgac
    32941 gaggtttatt cggtttcctg cgcagaaagg aggaagaaga aaaggaagct gtagtggagc
    33001 aacaacaaat taaattgcaa aggagggttg tccaggagga aacttccagg gaatggaaat
    33061 acaagaaaat tgaggggatc cattcaaggg ttatgaaatt agttggaacc aacggtggta
    33121 ttgtcgtagc aagtctctcc ggtctatttg aagtaaaagg taataacgaa ataccaatct
    33181 cttctgaacc tttcaccttt gttcatgcaa tgaagggaat tgacggggtt attgccgctg
    33241 gagcgaatgg tggcgtttcg tttttcagga agtcaggtga caactggtcc tcttatccaa
    33301 ctttttcgga gatagaagaa tatatccaat ttatttttca agattcaaag aacaatattt
    33361 ggctgcttgg tcctaatgta gcttataagg ctacactgtt tcaggatgtt attgataatc
    33421 ttgaattctg gcccatcgaa aacccctttt attccacaaa tattggaggg gaaatcaacg
    33481 gacaggtgta tatattaaac tcaaattcat tgtatacatt caatgaaatc acgaaggaaa
    33541 ttgagaagaa ggaatcaacg aatttgtgga gtaaatttcc tgagcgaaca atttcagatc
    33601 tggatggcac catctggatt tatgatggca aggattggag tcccttgggt agccaatacc
    33661 tcacctcccg caagataagg cccataagtc ttttcaacga agtctcattc ctgacctacg
    33721 ataaagattc cgattcattt tggataataa caggggagaa tgatttatat aaaatgtcaa
    33781 ataaagacac cattgtaagc aaccatcttc cattgttact tcgagaaatc aagaatggtc
    33841 aacgactcct cccgattgag ggtaattttg aaattcagca aaaccaaaat tctcttggtt
    33901 tcaattttct gaaggctgat tttgacaatc ttttcaaaat cgagtaccgc tataaactgg
    33961 aaggactaaa tgcatcatgg tctgagtggt ctggagattt caacaatatc agttttccat
    34021 ttctgcctga aggtaattat gttctaaatt tcgaaagccg tgatgtgttc ggccaaataa
    34081 atagtcttga gccattcaca ttcaaaattg ttccaccgta ttggaagcga ccctggtttt
    34141 atggagtcga gataatagca tttggaggat tgtttctctt gtccttccgg ctcaacagga
    34201 ttgatcataa atacaaattc ctcagcaaac ttcttgcatt ccttacttta attttggccg
    34261 ttgagttcat tcagatagtt gctgaactaa aattcccatc cggcaattca cctgttaccg
    34321 gtttcttcat ccaggtattc attgcccttg tccttcttcc tttagaagga gttgtaaggc
    34381 gtgcagtatt cagaatcgaa gaaacccaag acatgaaagc ttttaataag aaagccaaat
    34441 aacttcgcac tttttgattg aaaatttcta atattaggta agactaaatt cctatatcaa
    34501 tgaaatcatt ccttatcgct gcactgatcc ttttcctggg ggcaggttgc cagacggaaa
    34561 aacaatcaca gacaattact gaggagaatc caccaatgga ggattttaat ttcgaagaat
    34621 ctgattccaa tgcaattgtt attgccgaca gtgttatgaa gaaaatgggt gggaggaaaa
    34681 attgggatga aacacgatat ataagctgga cattttttgg aagacgaaaa ttgttgtggg
    34741 ataagtggga tggaaatgtg agaattgaat tcctgaatga tccttcaacg atctatttag
    34801 tgaatgttaa ctccatgcaa ggaaaagcta tgaaggccgg tgtggaaatt acccatcctg
    34861 acagtctggc aaaagaactt gaacgagcac aggctttatg gatcaatgac tcttactggc
    34921 ttttcatgcc tttcaaactt aaagacagtg gcgtaacatt aaaatacatt cgtcaagact
    34981 caacaattac cgggacgctg tcggatgtgt tgcaattaac ttttaagaat gtgggaaata
    35041 caccaaataa taaatatgag gtctgggtga atcgggatga gggattgatt acccaatggg
    35101 catacttcca aaattcagcc caggatagcg catcttttat caggccattt gataattatc
    35161 aaaagtatgg aaaaatattg ctatccggtg atcgctcaga taatggtggc ccgggaaata
    35221 taaaagtaat ggaatctgtc ccagaagaag ccttcacatc atttgatttc tctcaccaat
    35281 aagaagcccc tgatcggggc ttctacttca ctatgcgcta tgaacctgtg agggtaaatc
    35341 ctctctaatt gaggaaatag tgaattcttt gggttgattc tcaatttaca aacaagtttc
    35401 ttaaataatt agttctttta actctttttg aaggctatgc gtatctttag agtatggaaa
    35461 gagatgtttc caagctgttg aaagcccaac ttaaaaaact taattccaaa gattttgatc
    35521 ttgaagcatg gaaaagttcc acctccgccg tgttatccat agctttcggc gctgatgatc
    35581 ctcatgttca tgaaattcag aaattaaaaa tcgattacgg aagttgggcc ttgagagacg
    35641 ctaaagcttc ttacgaccct atggtaactt gtaaaaaaat aggcaaagag gttgttgaaa
    35701 tcgcgctgag tcaactaggt gacagggaag aattaacgtc tgatttaaat tccattttgg
    35761 aaagtcagtt aatggataaa tctctaaaag aaataaggaa ggttttgaaa tccagtaagt
    35821 ctgattcaaa gaaaggagaa aaactgcaag aaattctgaa aggtttggat aacgacaatc
    35881 tggcttctat tctcgcgcaa atgatcttga aaagcgagaa ttaaattcca cttacatctc
    35941 cagcctaaac taaaccagaa atattttgct cttcaacgca agttgaatct ctctcagtgc
    36001 ccttatttta aaatcatcat cagatcctgc tttttgaggg ctggtgaagt ttctaataaa
    36061 ggagagaacc ttaacttgct gatccttgtt taggctttcg agagatagca agatttcact
    36121 tcttgtatcc ttcattgctg ttagacatta gttcgttaat taaagtaaac gtatttaaac
    36181 cataattgtt agccacaaaa tgattagtaa tgaattcgta aagcatactt tttgttagat
    36241 taacattgcc aaaatcaatt attaacgaaa ttagaagcat gaaattaact aacgcctcaa
    36301 ttctggttct tttacttaca tcatactcta tagttgaaag taaccccaaa attgggccaa
    36361 ccatgaaatt aaacaatatg aagactgagg ttgcaacttt tggcgccggc tgcttttggt
    36421 gtgtcgaggc catttttcaa agtttgaaag gagtagaaaa agtagtttcc ggctattcgg
    36481 gtggaaaaat caagaatcct tcttatcggg aagttagttc aggacttagc ggccatgccg
    36541 aggtagttca aattacctat taccctgata ttattgctta taaggaactg ctggaagtct
    36601 tttggcaaac ccatgatccc actactttga acagacaagg tgcggatgtt ggaactcaat
    36661 accggagtgt aatttattat cacgatgaag agcagaaaaa cgcagcggaa ttttacaagc
    36721 aggaattaac gaaagccgga gtttttctga atcctattat tactgaaatt aaaaagtttg
    36781 aaggcttttt cattgctgaa gaatatcatc agaattattt catgttgaac tctgatgctc
    36841 cttattgcaa ctatgtaatt aagccaaaga tggagaagtt taaaaaagct tttgcagaga
    36901 agtataaaaa ggcagagata gataattaag aaacttagtg tttcacagga acaggatcat
    36961 ttgtgctttt tcgtttaatt agtcccctca ctaatttttg gtatctcaaa ctcccgaatc
    37021 agttgcccgt tatttgattt ccaggtacct ttcattacct gacttttctt atccacggtt
    37081 acattgaaaa caccataact ggattcggca gggtaaaact tctccggccc gccccaatat
    37141 ttttcaataa tttctctttt ccatgctggt tcctcgtcat aaggcaatct gtactcttcc
    37201 atagtaactt caaaatgatc gccactgtcg ccaaaagtgc cccgcatgtc tatttgttta
    37261 atgtcgtcct gggtggtaaa cttgttaaac ccagtgagtt ttctttccgg gttcacgttg
    37321 tcaaccggat aaatactaat cctaatctcc ttgtcggcga agcttccctg ccaggttcca
    37381 ttcaatatct tataaaatga atccggtgga gttggatgaa ttcttccggt tcggatttgg
    37441 ctgatgggtt cccatgatat tctggcaagg gggtgatcgc ttttctttaa tttttcagta
    37501 aggaaaaatt gctttcgagt aaacatttcc cttggcctta cttcattgaa tctgaattca
    37561 ttggcgatat ctcccaggtt ttcaacccag atcaggaacc g
//
LOCUS       DASWHX010000078        28036 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_60622, whole genome shotgun sequence.
ACCESSION   DASWHX010000078 DASWHX010000000
VERSION     DASWHX010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 28036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28036
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60622"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(77..1018)
                     /locus_tag="VGA21_05585"
     CDS             complement(77..1018)
                     /locus_tag="VGA21_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009580546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HEX9650012.1"
                     /translation="MKNRITELFGIEYPIIQAGMVWCSGWKLASAVSNAGGLGLIGSG
                     SMYPETLKEHVVKCKEATDKPFGVNVPLLYPDIDKIMKIIEEEQIKIVFTSAGNPTTW
                     TSTLKQMGATVVHVVSSVKFALKSEQSGVDAVVAEGFEAGGHNGREETTSFCLIPSVS
                     EVVKIPTIAAGGIGTGKGMMAAFALGAEGVQIGSRFAVSEESSAHINFKKRIVEAGEG
                     ETVLTMKKLTPVRIIKNSFYNLIKDAEDKCVSNEELSQILGRSRAKKGIFEGNLEDGE
                     LEIGQISGNIKDILPAREILKNIWEEFISTKKRLKDL"
     gene            1107..2873
                     /locus_tag="VGA21_05590"
     CDS             1107..2873
                     /locus_tag="VGA21_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013454283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEX9650013.1"
                     /translation="MKELLVKFLVWRIRHVKDKNFLLILGGIIGIIAGLAAVLLKLSV
                     HAIQKFLTQDFDVGYENYLYLSYPLVGILLTLVVAKFILKEKLGHGITQILYSISKKS
                     SIIRGAMMYSRMITSALTVGFGGSVGLEAPIVVTGSAIGSNLGRLAHLDYKKRTLLIG
                     CGAAGAISAIFNSPIAGVIFSIEVILMDVTIAAFIPLLISSVAASMVSIVLNQDQILF
                     SFRIQDPFMAIDLPYFILLGIVCGVVSVHFTRMTYAVEGIIKKVKNDYSRAITGGILL
                     GVIIFLFPPIFGEGYNTIKSLLSNDAEVLNTTLFFGQSSNPLVLILFIFGVVVIKPVA
                     SALTIGSGGSGGIFAPSLFMGGTAGFVFAETVNLVMGSEALSTSNFVLVGMCGVMSGV
                     MHSPLTAIFLIAEITSGYELFVPLMLVSAASFSVVSYFEKHSLYTKHLIEQGDLIKYD
                     RDLQVLSLINLKKIVEEDLLQIHPDAKLKELVNLVKVSKRNIFPVVKDQQLMGIITLD
                     DIREIMFDKDAQENQAVSSLMHDPPAEVSSMESMQSVMSKFEITGAWNLPVIDDGKYV
                     GFVSKSRIFNTYRQKLIRQDKQ"
     gene            3370..3846
                     /locus_tag="VGA21_05595"
     CDS             3370..3846
                     /locus_tag="VGA21_05595"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX9650014.1"
                     /translation="MMRIIILVLLLFCQPALSQEVEVVQFERIEKILKEDNPKLRIYN
                     FWATWCRSCIIEMPYFEEIAKSKRDKVEMNFISLDYADQVESTVKPFLKRKAITSRVY
                     LIDNLDYNSWIDKVDPRWSGALPATVFITPSGKKIFYEKEFKKEELEKIVESLLNQ"
     gene            3851..4456
                     /locus_tag="VGA21_05600"
     CDS             3851..4456
                     /locus_tag="VGA21_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009195341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEX9650015.1"
                     /translation="MKLYSTTLLIIASFLFGGSASVPYEIGDFVADFKLKNIDGKMIA
                     LTDKKEVKGYILVFSCNTCPVVKQYEQRIMDLHEMFASKGYPVIAINPNDPARSPGDS
                     FDEMAKRSKNMGYKFPYVFDETQEVARAFGATNTPQIYILNNEKGKFKLMYKGAIDNN
                     QRSAAAATERYVETAMTSLLKGEEVKVPVTKAVGCMIKWSE"
     gene            complement(4572..6101)
                     /locus_tag="VGA21_05605"
     CDS             complement(4572..6101)
                     /locus_tag="VGA21_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010608305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HEX9650016.1"
                     /translation="MSILNSILTWVMRKRIHQIELFIKYPHDVQEELLNGLLSSAAKT
                     EFGQSYEFGGIETVEQFKERVPLHFYESFFPYVERLMKGEQNILWPSEIKWFAKSSGT
                     TNARSKFIPVSPEALEDCHYKGGKDLISIYVNNNPDSEMFSGKGLAIGGSHQINPFDT
                     SSTSYYGDVSAVIMQNLPLWAQFVRTPSLEIALMDKWEEKIEKMALVTSEINVTNMAG
                     VPTWTVLLLQRILELRNKKSIAEVWPGLEVFFHGAVSFTPYRQLFDSLIGKPMHYVET
                     YNASEGFFGIQDRSVDGEMLLMLDYGIFYEFIPIEHDGEENPKTLGLHEVELDKIYAM
                     VISTNAGLWRYRIGDTIRFTSLSPYRVKISGRTKHFINAFGEEVVIENAEAAITKACR
                     ETNAIMDNYTAAPRYLDVGQKGSHEWIIEFIRQPSDMEKFIEILDKTLREVNSDYDAK
                     RYKDIALDKPLVHNVKEGTFYEWMKKRGKLGGQNKVPRLSNDRQYLDDILAMISVPVD
                     L"
     gene            complement(6205..7845)
                     /locus_tag="VGA21_05610"
     CDS             complement(6205..7845)
                     /locus_tag="VGA21_05610"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9650017.1"
                     /translation="MNNFLLVFAGLLVIPFISRSQLQEFEIKEVNLIDQINQLNKTAS
                     EVYDSVPEMSFKLANMAMNLAREQNFDAGVAEAHLNLGNYYKLKLLQSKRLDNYSSAL
                     AIFKNLNDTINLALTYKLIGSVYASEGLIDKMLDNLNESIALYSQQNDSDGIYSCYLE
                     MGNGYYWAKDYSNSTKYYLLSLSSLEGVNDSQNRLAGIFNNLGVNHLEQGNYSKALDY
                     FRQSYSIYYGTNNLRRLTANYYNSARALIGMAEYDSAMTFAIKSHELSLNLKIDAALV
                     ESTQLLAELFEEARNYQEAYKYQKMHSQLVDSIRNVETARVIDNYASALEIEEAKLLI
                     EVARKEHLITKIYRNLAAVGFFLVVLFSITGFLIWRGRHHKKMANFDAQKKLVEAEVE
                     NLSLRKKLINDELEFKNREVTVYSMKAVQKNTFLKEIRTIVDQIVEENQQETPNLRKL
                     LNLIDSNSTTDKGWDEFKKSFEKVHQDFFSRIKEKHPDVTSKELEHCALIKLNRNLKE
                     TASVMGITSESVKMARHRLKKKLNLGPEESLVDYLIKF"
     gene            complement(7919..8650)
                     /gene="lptB"
                     /locus_tag="VGA21_05615"
     CDS             complement(7919..8650)
                     /gene="lptB"
                     /locus_tag="VGA21_05615"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015265168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HEX9650018.1"
                     /translation="MKLRADHLYKIYRKRTVVNNVSLEVNRGEIVGLLGPNGAGKTTS
                     FYMIVGLIKPNDGRIFLEDQDITSLPMYKRAKLGIGYLAQEASVFRKLTVEENLLAVL
                     EMSKLSRHEQREKVESLIEEFSLGHVRKNIGMVLSGGERRRTEIARALAVDPKFVLLD
                     EPFAGVDPIAVEEIQSIVAQLKQKNIGILITDHNVNETLSITDRAYLMFEGRLLKAGS
                     AEELAADPQVRKVYLGKHFELKRKI"
     gene            complement(8647..10359)
                     /gene="recJ"
                     /locus_tag="VGA21_05620"
     CDS             complement(8647..10359)
                     /gene="recJ"
                     /locus_tag="VGA21_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002704666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEX9650019.1"
                     /translation="MNKKWIFLPDDGEKRVREFAATLNVHHAIASILIQRNIDDFEKA
                     KAFFRPSLENLFDPFLMKDMDVAVERLCMAISSKQKILVYGDYDVDGTSAVALVYSFL
                     RKFHSAIEFYIPDRYKEGYGISDQGIQFAIDSGIKLVICLDCGIRSVELIEKARNNGV
                     DFIVCDHHKPGSTLPPAVAVLDPKREDCGYPYDELTGCGVGFKLLQGFCIRNKIDHEK
                     LFAYLDLLAISIAADIVPITGENRILTFHGIKLLESKPRPGLRALLKASGISGKISVM
                     DLVFGIGPRVNAAGRIHHARDSVNLLISDSMDSSMKLAETLNAHNSKRKEFDSSITEE
                     ALAMIEESDRLLNAKSTVLFKPDWHKGVIGIVASRCIERYYRPTIILTQSEGTATGSA
                     RSVEGFDIHEAINNCSDLLMKFGGHTHAAGLTMHLDNVQSFVQRFESEVSSTITIEQL
                     TQKIIIDHYLPLDELNSNLYKILRQIGPFGPGNMLPVFYCDDVVCTGTPRILKDKHLK
                     MFVKQQNGKRKWEAIGFNLSEHFNEISSGQPFLMAYSLDENQYMGQTFLQVTIKDIKF
                     GPVK"
     gene            complement(10446..11081)
                     /locus_tag="VGA21_05625"
     CDS             complement(10446..11081)
                     /locus_tag="VGA21_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009581734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ezrin/radixin/moesin family protein"
                     /protein_id="HEX9650020.1"
                     /translation="MRTISALVLCLALIFSFNATHAQLSKQEKKAWAKKAKEYKKNPE
                     SLKNIEEENQSLKSQVSSLNTQLNNLNSQTADKDAKISELQDQLNRSRADLASAQESV
                     RKMRAEMEAKPPAGNWDQGLVFKVQIGAFKNKDLSAFFEGNPNITGFTAEDGSQRINF
                     GNFRDYWTADTLKKHLREMGIKDAWVVPFEDGQRVDLKKVLEGIQPEKKSE"
     gene            complement(11147..12550)
                     /gene="fumC"
                     /locus_tag="VGA21_05630"
     CDS             complement(11147..12550)
                     /gene="fumC"
                     /locus_tag="VGA21_05630"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014682455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HEX9650021.1"
                     /translation="MEYRIEKDTMGEVKVPADKYWGAQTERSRNNFKIGSEKEKMPIE
                     IIYAFAYLKKAAAITNFELGVLPREKNELIGRVCDEILSCSLDDQFPLVVWQTGSGTQ
                     SNMNVNEVISNRSHVLSGNKLGDGNPAVHPNDDVNKSQSSNDTFPTAMHIAAYKMVVE
                     TTIPGIKKLRDTLYAKSKKFMNDVKIGRTHLMDATPLTLGQEFSGYVSQLDHGIRALE
                     NTLEHLSELALGGTAVGTGINTPQGYSEKVAEKIANLTGLPFITAPNKFEALASHDAI
                     VESHGALKQVAVSLMKIAHDIRMLASGPRSGIGEIIIPANEPGSSIMPGKVNPTQAES
                     LTMVCAQVVGNDVAVTTGGMNGHFELNVFKPVMIHNFLTSARILGDACVSFNDHLAVG
                     IEPNQANLKKNLDNSLMLVTALNTHIGYEKAAKIAKTAYENGTTLKEEALNLGYLTAE
                     EFDKWVVPKDMIGSLKN"
     gene            complement(12614..13150)
                     /locus_tag="VGA21_05635"
     CDS             complement(12614..13150)
                     /locus_tag="VGA21_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010852537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEX9650022.1"
                     /translation="MRKLKLDELGRLSTQEFRSAPKVPFVLVLNNIRSLNNVGSAFRT
                     ADAFRIEKVILSGITGKPPHREIQKTALGATETVEWSYEKEGVEAVKKLKNQGYKIIS
                     VEQTTESIPLHRLEIESNSKIAAVFGNEVEGVDLEIIRESDQIVEIPQLGTKHSFNVA
                     VSIGIVLWDMALKGGYIK"
     gene            13243..15843
                     /gene="mutS"
                     /locus_tag="VGA21_05640"
     CDS             13243..15843
                     /gene="mutS"
                     /locus_tag="VGA21_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008197841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEX9650023.1"
                     /translation="MSKTKTGRETPLMKQYNLIRAKYPGALLLFRVGDFYETFGEDAI
                     TASKILDIVLTRRANGAASHIELAGFPHHALDTYLPKLVRAGYRVAICDQLEDPKMVK
                     GIVKRGVTELVTPGLSFHDNVLEKRQNNFLASVHFGEKNLGISFLDLSTGEFLTSSGN
                     AEYASKLLQSLNPSEIIFSKANKGNLKEIFGDKYNYYHLEDWVFQYDYTYEKLTNHFK
                     TTTLKGFGIESFPESIISAGAVLYYLEETEHNEISHISSVSRIEEEKYVWLDKFTIRN
                     LELVYPQQEGGIPLIQVLDNTVTPMGSRLLKKWIVLPLKEKDLIEERLRIVESLINEE
                     TGSEEILKNLRQISDIERLVGKVSAGRINPRELLQLKRALKHTLPIKEILKRSANKDL
                     QKLGELINPCDFVIQKIESELSDDPPMLLHLGKVMKKGVNAELDELREIAYSGKEVLV
                     QIQQREIKQTGISSLKVAYNKVFGYYLEVTNVHKDKVPSNWIRKQTLVNAERYITEEL
                     KVYEDKILTAEEKITSIEQKLYFDLVRQVSDFIVQIQENARILAKLDCLLSFAETATK
                     SKYCKPEIDDSLELEIIDGRHAVIEKQLPTGEEYVPNNLYLDNHSDQILIITGPNMAG
                     KSALLRQTALIVLMAQMGSFVPASTARIGLIDKVFTRVGASDNLSRGESTFMVEMIET
                     ASILNNISERSLILMDEIGRGTSTYDGVSIAWSIVEYLHKITTRPKTLFATHYHELNQ
                     LSEDLPRIKNFNVSVKEVGDRIIFLRKLKPGGSEHSFGIHVAQMAGMPNSIVLRADEI
                     LHFLEKDKKKSQEKKMREVPRDDFQLMLFDNDPKIKQVKEMLDALDINTMSPVEALLK
                     LNEFKGLLGK"
     gene            complement(15866..16732)
                     /locus_tag="VGA21_05645"
     CDS             complement(15866..16732)
                     /locus_tag="VGA21_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020600982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HEX9650024.1"
                     /translation="MRLNSLMILLVVVLTRVNAQDLPAYKIYSSEGKPSDYSKMIDGA
                     AEAGIVFVGELHNNSIAHWLELKITESIYAENSELVLAMEMFEADDQIVLTEYLDNVI
                     KEDHLLKEAKVWNNYKTDYKPLVEFARKNQLPVIASNIPRRYANLVYRQGLSSLDSLT
                     SEAKQWIAPLPIKIDLELPGYKSMIAMMGSHGDGDPNNIAFAQAVKDATMAHFILQNK
                     TLSNKILHFNGSYHSQNHEGIVWYIKQSRPDLKVITIQLLEQKDISYLDNDNLGKADF
                     IIVIPSDMTKTY"
     gene            16963..18201
                     /locus_tag="VGA21_05650"
     CDS             16963..18201
                     /locus_tag="VGA21_05650"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HEX9650025.1"
                     /translation="MKNLVILGAGTAGTIMANKLYKVLNKDEWKITIVDRDNLHYYQP
                     GFLFIPFGVYKPEEVIKPKADFLPDGITFIINNVKRVKPEKNEVIFEDGKIIEYDLLI
                     IATGTTPVPEETDGLKGKLWYKDIFDFYTYVGSVNLAKKLETWEGGKLVINLAESVIK
                     CPVAPLEFAFLADAYFIEKGIRDRVEIFYVTPLSGAFTKPKATEMLGQLMEEKNIKVI
                     PDFYLMRVDEDRKVIISYDEKEVPFDLLVSIPVNKGDEMIANSNLGDEDGLNFVPTDI
                     HTLQSKQFDNVFVIGDSTNIPASKAGSVAHFEADVLTENIISFINNKPLEAKFDGHAN
                     CFIETGFGKATLIDFNYDTEPLPGKFPFPIIGPMGLLKVTRSNHLGKLLFKWVYWHLL
                     LTGKKLPVSTEMSMAGKVNS"
     gene            18220..18531
                     /locus_tag="VGA21_05655"
     CDS             18220..18531
                     /locus_tag="VGA21_05655"
                     /inference="COORDINATES: protein motif:HMM:TIGR03342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TusE/DsrC/DsvC family sulfur relay protein"
                     /protein_id="HEX9650026.1"
                     /translation="METLTLDRQEIERNVEGYLKDASQWNEELAKEIAAEYNFILTEK
                     HFEVLRWLRGKQSEGVTLSIRKVGASGLVDIKQFYQLFPGGPLKVSSKIAGIPKPVSC
                     I"
     gene            18554..19093
                     /locus_tag="VGA21_05660"
     CDS             18554..19093
                     /locus_tag="VGA21_05660"
                     /inference="COORDINATES: protein motif:HMM:NF025066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HEX9650027.1"
                     /translation="MVETLEKTPVTATVEVPVKQERDTVKKVLIICSKGTLEDVYAAL
                     IMANGALMDGRDAKLFFTFFGLNAITKKNADHLHTATVGNPAFMPAMPTLVAGLPGFE
                     AFASYMMKKEMDKLDIPPVTEFFQMIDAAGGEIYACKLAAEMFKLNKEDFLDEVKDII
                     TVGDMYALADGDGSHLIFI"
     gene            complement(19232..19317)
                     /locus_tag="VGA21_05665"
     tRNA            complement(19232..19317)
                     /locus_tag="VGA21_05665"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19281..19283),aa:Leu,seq:taa)
     gene            complement(19365..19437)
                     /locus_tag="VGA21_05670"
     tRNA            complement(19365..19437)
                     /locus_tag="VGA21_05670"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19402..19404),aa:Gly,seq:gcc)
     gene            19614..20282
                     /locus_tag="VGA21_05675"
     CDS             19614..20282
                     /locus_tag="VGA21_05675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650028.1"
                     /translation="MKLNVYVTIALLPLSFALNAQSFVTPLDGTSLGMSKSNRVTTKD
                     GKVHEGKIVFSTYAAGQISGFTLKTETEKLKFKAEEIALLESEPGKLQKFSMATEGRT
                     VYSALAKDYSYIQELKYVTFEPVEIKEKVKLMQLLNPGFDEYIKVYLDPTAKENQWTG
                     QANSYFVKKGDEAIYLKKGKYDDMFWELFSGCEKFKENFEGELKFYDFPSHIALYNES
                     CPKE"
     gene            complement(20373..21044)
                     /locus_tag="VGA21_05680"
     CDS             complement(20373..21044)
                     /locus_tag="VGA21_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014018538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEX9650029.1"
                     /translation="MELKKNKAYELTKLRGLFFNIGLVITLIAVITAFEWKFYDDGQL
                     VSLGTVNDDFEEMLEIPPTEQPPPPPPKIQQPIIVEIPDDEVIEEIPIDLDIEITEES
                     VVQEIILEAAPQEEEVEEIFTIVEEMPSFPGGVYAFHEYLRKNLHYSPSARKMGVEGK
                     VFVQFVVDKKGNISEVEAIRGIGAGLDEEAVRVVKNSPAWNPGKQRGNAVKTRVVIPI
                     NFALN"
     gene            21534..24629
                     /locus_tag="VGA21_05685"
     CDS             21534..24629
                     /locus_tag="VGA21_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018620459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEX9650030.1"
                     /translation="MKKLLILLIISGLFIIDVAGQGNDPYNEKFYNTLEWRNIGPERG
                     GRSLGCSGSPGRPNEYYFGATGGGLWKTVDGGQNWFPVTDGQITCSSIGAVAVAETNP
                     DVVYIGGGEIQLRGSITQGDGVYKTVDGGKTWRHLGLAETQAISRIRIHPTNPDIVFV
                     AALGHPYGDNQERGVFRSTDGGNTWQKVLFVSEKAGAADLIIDRTNPKVIYASTWQVY
                     RKAWKMWGGGPDSKLFKSVDGGDNWIELTKNPGMPEGPIGKIGVTVSPADPNRVWAIV
                     EANEGGVFRSDDGGWTWQKTNDERKLRQRAFYYSRIYADPWDREVVYGLNTGFYKSTD
                     GGKTFDVTIRVPHGDNHDLWIDPNNPDRMINSNDGGGNVSINGGRTWTEQDYVTTQLY
                     HVMTTSDVPYHVAGAQQDNSTIAIPSDGWGNMQARGPNHGWYYAVGGGESGWISQHPV
                     NKDIFYAGSQGALLTRYDRSNGQIRDIQVYPRFFSGEPASALPERWQWTFPIVFSRQD
                     PNIMYTCSQHVWKTMDDGQSWIKISPDLTYADPETLGPTGGIITMDMNGPEIYATVFA
                     LEPSHHDINTLWVGSDDGKIHITRDGGKRWSDITPKDMPKFSRVSIIDESEHSPGTAY
                     VAANRYQVDDRAPYVWRTIDYGKTWTKITNGIKDGHFARAVREDHVQPGLLFLGTEHG
                     AYVSFDAGENWKPLQLNLPDTPIRDLVVKDNDVVLGTHGRGFWILDDIQPLRELGPQL
                     AQKNAHLYKPADPIRGIYNANFQYYLKNPVDTIKVEILDAQDQVIRTFIGTETSNRVD
                     PNTPWWAGGSGSPATAKGLNAFEWDLTYEGATIFDGMIIWSARPQRGPKAPLGEYKVR
                     LTSGNYSETRNFTIKINPNLKGITPEDQKLQFDLAMKIRNKTTAANEAVISIRKIRKQ
                     IEDRIANSGDEQLNTSARAFISKITTVEEDLYQVKNQSNQDPLNFPIKLNNRLASLRR
                     SVETGDAKPTNGAYKVFEELSKELEGHLTKLDQLIKNDLSVLNQSFQSKNLKPVTVGK
                     D"
     gene            24717..26468
                     /locus_tag="VGA21_05690"
     CDS             24717..26468
                     /locus_tag="VGA21_05690"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HEX9650031.1"
                     /translation="MYKNNSKILILAAAFLCLTCGNYQSETERSREIVNELADEYIES
                     YFDAYPFMALLSNAPDLHPEKLADNSREGIAQLEKKQLQLLTRLNAINPELMDSETQN
                     TYFILKNLLENSLAICTCRMELWNVSPTWTGWQSLFSLMASLLPIETPEQKNKAIKRL
                     SQIPVYLEMEIRNLRAGIQLNYSAPANNVRVVIEQMNALIAGGPENLPYTNIAGESDL
                     NFKEKLESLASLKIIPAIENYRDFLQDEYLIKTRTSFGVNENRDGIECYKASLKYYTT
                     TNFSPKEIHELGLMLVAGILAEVKNLGQKVMGIRDVRKIFFLMNSEPSFRFNNREEIL
                     INAERSIESVRAKLQDWFNVVPETKIKVQPFPDFQEKFAPAGEAVAAPEEGGTGLFYI
                     NTYNANEQSRAGLQSIVFHESIPGHLFQFAVAAGKKSHPICKYFYLSGHGEGWALYAE
                     RLADEMNLYSSDVERLGMLSNQLLRAARLVVDTGIHAFSWSRDESIDYLMSNAGLQRS
                     AAEAEVDRYIAVPGQATAYMVGYLEIINLRDAAEKKLGKAFVLKEFHDLMLEEGTLPL
                     WLLREKAESWIQRNIQK"
     gene            26562..28025
                     /locus_tag="VGA21_05695"
     CDS             26562..28025
                     /locus_tag="VGA21_05695"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEX9650032.1"
                     /translation="MSRGKRIAKINMSGEEFRKLGYELTDKIAKLLDEIDKKKVTSGL
                     SPVKIRELLNTDIALKDSGSKSEEIIDRAAALLLEHSLFNGHPRFWGYITSSPAPLGI
                     LADYMASAINPNVGGWTLSPVATEMEVQTIKWIGELINYKADGGILVSGGNMANFVGF
                     LAGTRAKLGQDIRRLGLAGRTPKIYCSAETHTWIQKAADLFGFGTDATVWIPTNENQQ
                     MNADELQKRIEKDKKHGSEPIMVIGTAGSVSTGAIDPLYEIGAICREHNLWYHIDGAY
                     GGFAACLPERFPELEAFQDADSIAVDPHKWLYAPLEAGCTLVKYAHTLPDTFSYHPPY
                     YHFGVEAVNFVDNGFQNSRGFRALKVWMMLQQVGSGGYKEMIAEDCDLAKHLYEILPR
                     YKELERFTYNLSITTFRYIPEELRKKAKEESTSKYLDDLNKNIQAKLESGGDFFVSNA
                     VINGIYVLRMCIVNFRTSKFDVEALPERVIELGRELH"
BASE COUNT         8357 a         5604 c         5846 g         8229 t
ORIGIN      
        1 ttagccattg tttctccatg gatatttcct aataagacgg tgagtaacca tccgaaggat
       61 attgagaaag gatagtttat aggtctttta atcttttttt ggtcgaaata aactcttccc
      121 agatattctt caaaatttcc cgtgctggga gaatatcctt tatgttgcct gagatttgcc
      181 caatttcaag ttcaccatct tccaggttgc cttcaaaaat tcctttttta gctctgctac
      241 gacccagaat ttgagataat tcttcgttac ttacgcattt atcttctgca tctttaatga
      301 gattgtaaaa tgaatttttg attattctga caggtgttaa ttttttcata gttaaaacag
      361 tttcaccctc accggcttct actattcttt ttttgaaatt aatgtgggcc gaactttcct
      421 cgctgacagc aaacctgctc ccgatctgta caccctcggc ccccagcgcg aaggcagcca
      481 tcattccttt gccagttcct attccgcccg ctgctatcgt aggaatttta actacttcac
      541 taacggaagg tatcaggcag aaactagtag tttcctccct gccgttatgt ccccctgcct
      601 caaatccttc cgcgactacc gcatcaactc ctgactgctc acttttcaat gcaaatttta
      661 cactggaaac aacatgaaca actgtcgccc ccatttgctt caacgtagaa gtccaggtgg
      721 tgggatttcc ggcggaggta aaaactattt tgatctgttc ctcctcaatg attttcataa
      781 ttttatcaat atcaggataa agcaggggaa cattaacgcc aaaaggctta tcagtagctt
      841 ctttacattt aaccacatgt tcctttaacg tttcaggata catcgagccc gagccaatta
      901 aacccaaacc cccggcattg cttaccgccg aagcaagttt ccagccgctg caccaaacca
      961 tacccgcttg tataatggga tattcaattc caaaaagttc tgtaatcctg tttttcatac
     1021 aaacagcgtt tttaaaagtt tctgctttat ttattgaatc aatatataaa tttgaaggat
     1081 gattattact ggcttgtctg tataaagtga aggaactcct ggttaaattc ctggtttgga
     1141 gaatcaggca cgtgaaagat aaaaatttcc tgctgattct tgggggtata atcggaatta
     1201 ttgccgggtt ggctgccgtc ttacttaaat taagcgtcca cgccattcaa aaatttctta
     1261 cccaggattt tgatgtaggt tatgagaatt acctgtacct aagttatcct ttagttggga
     1321 tcctgctgac tttggtcgtt gcaaagttta ttctgaaaga aaaactcggg cacggcatta
     1381 cccaaattct gtattccatt tcaaagaagt caagtattat aagaggagca atgatgtatt
     1441 cacgaatgat aacatccgcg ttgacggtag gttttggagg ctccgttgga ttggaggctc
     1501 ccatcgtggt tacaggatct gcaattggct ctaacctggg cagattggcc cacctggatt
     1561 acaaaaaaag aactttgcta attggctgtg gggccgcggg tgccatttcc gcgattttta
     1621 attcccccat tgccggagta atttttagca ttgaagttat tctgatggat gtaaccattg
     1681 ctgcatttat cccactgctc atttcatcgg tcgccgcttc aatggtttca attgtgttga
     1741 atcaagacca gatattgttt tcatttcgaa ttcaggatcc ttttatggcc atagatcttc
     1801 cctacttcat actgcttgga atagtatgcg gagtagtttc cgtccatttt acccggatga
     1861 catacgcggt ggaggggata ataaaaaaag taaaaaatga ttacagcaga gctattaccg
     1921 gcggaattct tctgggagta ataatatttc ttttccctcc catttttggt gaaggatata
     1981 ataccataaa atcactcctt tccaatgacg ctgaagtatt gaatacgaca ctcttttttg
     2041 gacaatcttc aaatcctctg gttctgatat tatttatctt cggtgtggtg gttattaagc
     2101 cggtcgcctc cgcccttacc attggctcag gaggcagcgg tggtattttt gccccttctc
     2161 tttttatggg aggaaccgcg ggtttcgttt ttgcagaaac tgtaaacctg gttatgggtt
     2221 cagaggcgct gagtacttct aattttgtac tggtaggtat gtgcggagta atgagcggtg
     2281 taatgcattc cccgctaaca gcaatattcc ttattgcaga aattacatct ggctacgaac
     2341 tctttgttcc gcttatgctg gtatcggcag catcctttag cgtggtttct tattttgaaa
     2401 aacattcact ttacaccaaa catcttattg agcaagggga tttgatcaag tatgaccgtg
     2461 acctgcaggt tcttagttta ataaatctta aaaagatcgt ggaagaagat ctgttgcaga
     2521 tacatccgga cgcaaaactc aaggagttgg tgaatctggt taaggtttca aaaagaaaca
     2581 tcttcccggt ggtaaaggat caacagttaa tgggcatcat aaccctggat gatatccggg
     2641 aaatcatgtt tgacaaggat gcacaggaaa accaggcagt cagctcattg atgcatgacc
     2701 cgccggctga agtttcttcc atggaaagca tgcagtctgt gatgagcaag tttgaaatta
     2761 ccggcgcctg gaatcttccg gtgatcgatg atggcaaata tgtagggttc gtaagcaaat
     2821 caagaatctt taatacatac cgtcaaaagc ttatcaggca ggacaagcag taaaaatgct
     2881 aattaaaggc aaggtaatct tggtaaaatt caatactctt taaaggcaat tccatgataa
     2941 gcaggagttt agctattcta acctcaaatt acattgattt ctgtgctgtt ttcagttgtc
     3001 cacaaagttc aaatttaaaa aggacaccgt aaattgttga ttcataatga attaaacaca
     3061 tgaagcaggg aattgtggat aattaataaa aaatgtggat aatttcagat cgatggtttt
     3121 tagcctcctt atctcatcac ctaaccaccc cttacaccaa tcaaggagtg aaaacccggg
     3181 cttctgcaag ttatatgcat tattgaataa gtataataat attggcaaat tagcgcctcc
     3241 aataccagcg tttgagaaag gaaggctcaa aaaccgttgc tgtatttggt cttgcgaaat
     3301 ggttattgaa attctttctt tgaaaccgga aggtgaaata gacggtaaaa tttttgcttg
     3361 taacaatcaa tgatgagaat aattatcctg gttttactat tgttttgcca gccggctttg
     3421 tcgcaggagg tggaggtagt acaatttgag agaatagaga aaattttgaa ggaggataac
     3481 cctaagcttc gcatttataa tttttgggcc acctggtgca ggtcttgcat catagaaatg
     3541 ccttattttg aagaaattgc taaaagtaag cgggataagg tagaaatgaa ttttatcagt
     3601 cttgattatg ccgaccaggt agaatcaacg gtgaaacctt tcctaaagag gaaagctatt
     3661 acctccaggg tttacctgat agataacctg gattacaact cgtggataga taaagtagat
     3721 ccccggtggt caggtgcatt accggctacg gtatttatta ctccttcggg aaagaaaata
     3781 ttttatgaaa aagaatttaa gaaagaagaa ttagaaaaaa ttgttgaatc acttttaaat
     3841 caataaacct atgaaacttt atagtactac gttattaatt attgcctcat tcctttttgg
     3901 agggtcagcg tctgttcctt atgaaatagg agactttgta gctgacttca aactgaaaaa
     3961 cattgatgga aagatgatag ctcttaccga taaaaaagag gtaaaaggat acattctggt
     4021 tttcagttgc aacacctgcc cggtggtaaa gcagtatgag caaagaatta tggatttgca
     4081 tgaaatgttt gcgtctaagg gttatccggt aattgccatt aatccgaatg atccggcccg
     4141 ttcaccaggc gattcttttg atgagatggc aaagaggtcg aaaaatatgg ggtataagtt
     4201 tccgtatgtt tttgatgaaa ctcaggaagt agcgcgagcc tttggtgcca ccaatactcc
     4261 gcaaatctac atactcaaca acgagaaagg gaagttcaaa ttgatgtaca agggcgctat
     4321 cgataacaac cagcgaagtg cagctgcagc aaccgaaaga tacgtagaaa ccgctatgac
     4381 ttcattgcta aaaggcgagg aggtaaaagt acccgttacc aaagctgtcg ggtgtatgat
     4441 caagtggagc gagtaaggga tctgtccggc acgggaattc gttatggggt caggtgcctg
     4501 gcatgaagca tgccacactt attttcattg accctgttaa ctaaaagtgt ggcacacttc
     4561 acacttcctc tttagaggtc cacgggcaca gaaatcatgg ccagaatatc atcgaggtat
     4621 tggcgatcgt tagaaagtcg cggcacttta ttctggcctc ccagcttccc ccgcttcttc
     4681 atccattcgt agaaggtacc ttcctttaca ttgtgaacaa gcggcttatc caatgctatg
     4741 tctttatatc ttttggcatc gtaatctgag tttacttcac gcaaagtttt atcaagaatt
     4801 tctatgaatt tttccatatc tgaaggttgc ctgataaatt cgattatcca ttcgtggcta
     4861 cccttttgcc ctacatcaag ataccgtgga gcagctgtat agttatccat gatggcgtta
     4921 gtttccctgc aggctttagt aatggcagcc tctgcatttt caatcacaac ttcctcacca
     4981 aacgcgttaa taaaatgctt tgttcttccg cttattttta cgcgataagg agaaaggctc
     5041 gtaaaacgga tcgtatcacc gattctatac cgccacaacc ccgcattggt actgattacc
     5101 atagcgtaaa ttttatctaa ttcaacttcg tgaagaccaa gcgttttagg gttttcttcc
     5161 ccgtcgtgtt caatcggaat aaattcataa aaaatcccat aatccagcat caatagcatt
     5221 tcaccatcaa cacttctatc ctgaatgcca aagaaacctt cagacgcatt gtaggtttcc
     5281 acataatgca tgggtttccc aattaatgaa tcaaataatt ggcggtaggg agtaaatgaa
     5341 acagcgccat gaaagaaaac ttcaagtcct ggccatactt cagctatcga tttcttattt
     5401 cgtaattcca gaatccgctg tagtaaaagt acggtccagg taggaacgcc ggccatattt
     5461 gtaacattta tttccgaagt aaccagagcc attttttcaa tcttttcttc ccatttatcc
     5521 atcaatgcga tttccaatga tggagttctg acaaactgtg cccaaagcgg caggttttgc
     5581 attattacag cggaaacatc tccataataa gaagttgaag atgtatcaaa aggattaatt
     5641 tgatgactgc cgccaattgc cagtcctttc cctgaaaaca tttcactgtc aggattattg
     5701 tttacataaa tactaatcag gtctttgccg cctttgtaat ggcaatcttc aagagcctca
     5761 ggacttaccg gaataaactt agagcgggca tttgttgtgc cggaagactt tgcgaaccat
     5821 tttatttcgg aaggccacag gatattttgt tcccctttca tcagcctttc tacatagggg
     5881 aaaaacgatt cataaaagtg aagaggaact cgttccttaa attgctccac agtttcaatt
     5941 ccgccaaatt cataactttg tccgaattca gttttagctg ctgaactcaa taatccattt
     6001 aataattcct cctgcacatc gtgtggatat ttaatgaaaa gctcaatctg atggatgcgc
     6061 tttctcatta cccaggtcaa tatcgaattg agaatactca tgtgttccct ttgctgatta
     6121 atataataaa attgggtggg aaaaggatga aaagtatcca ttaatataaa taaaaatttt
     6181 ataaaggata gtcatgcaac tgaactaaaa tttgatcagg taatcaacca ggctttcctc
     6241 tggtcccagg ttcaatttct tttttagcct atgcctggcc attttaacgc tctcagaggt
     6301 aattcccatt acagaggctg tctctttaag gttgcgattt aatttaatga gtgcgcagtg
     6361 ctccagttct ttagaagtaa catccggatg tttttcttta attcgtgaaa agaaatcctg
     6421 atgaaccttt tcaaaggatt tcttaaattc gtcccaacct ttatctgtag tggaattaga
     6481 gtcaatgagg tttaatagtt ttcttagatt gggggtttcc tgttgattct cttccacaat
     6541 ttgatcaaca attgtccgga tttcctttag aaatgtattt ttttgaaccg ccttcattga
     6601 ataaacagta acttcacgat ttttgaattc cagttcatca ttaatcaact tttttctaag
     6661 agaaaggttt tcaacttcag cctccaccaa ttttttttga gcgtcaaagt ttgccatctt
     6721 cttatggtgc cttcctctcc aaattagaaa tccggttatg gaaaaaagta ctaccaaaaa
     6781 gaaacccact gctgcaaggt ttctgtaaat tttagtaatt aggtgctctt ttctggctac
     6841 ttcaattagc agttttgctt cttcaatttc gagggcagat gcataattat ctataactct
     6901 tgccgtttct acgtttctga ttgaatccac cagctgtgaa tgcatttttt ggtatttata
     6961 tgcttcctgg taatttcgtg cttcttcaaa taattccgct aaaagctgag tggattctac
     7021 aagcgcagca tctattttca aattcaaact taattcatga ctcttaattg caaaggtcat
     7081 agctgaatca tactcagcca tgccaataag tgcgcgggct gaattataat aattagctgt
     7141 gagtctcctt aaattattgg ttccataata aatcgaataa gattgcctga aatagtcaag
     7201 cgctttggaa taattaccct gttcaaggtg attcactcca aggttattaa atatccctgc
     7261 aagccggttt tgcgaatcat taactccttc cagggaagaa agggaaagta aataatactt
     7321 agtagaatta ctataatctt tagcccaata ataaccattg cccatttcca aatagcaaga
     7381 atatatgcca tcgctgtcgt tttgttgtga gtagagggcg atagattcat tcaggttatc
     7441 cagcatttta tcgatcaatc cttcagatgc ataaacactt ccaattagct tataggtaag
     7501 ggccaggttg atggtgtcat taagattttt gaaaatagct aacgctgagg aatagttgtc
     7561 gagccgtttg ctttgaagta acttgagttt gtaataattg ccaaggttaa ggtgtgcttc
     7621 ggcgacacct gcatcaaaat tttgttctct tgcgaggttc attgccatgt ttgccaactt
     7681 gaatgacatt tcaggaacgg aatcatagac ctccgaggcc gttttgttaa gttgattaat
     7741 ctgatcgatg agattgactt cttttatttc gaattcctga agctgactcc tggatatgaa
     7801 tgggatcaca agcagccccg cgaatacaag caggaaatta ttcataagtg aactcaggca
     7861 acatcatgtg ctgccttact aaatatatca aaatttggtg agaagagggg taactatttc
     7921 aaattttgcg cttcagctcg aaatgtttgc cgaggtaaac ttttcttacc tgtggatcgg
     7981 cggcaagctc ttcagcgctt cctgctttaa gtaatctccc ttcgaacatc agataggcac
     8041 gatctgttat ggaaagagtt tcgttaacat tgtgatcagt tattaatatg ccgatgttct
     8101 tttgctttaa ttgagctaca atcgactgaa tttcttctac tgcaattggg tcaacgccgg
     8161 caaagggttc atccagcaaa acaaattttg gatccacagc taatgctctt gctatttctg
     8221 ttcttcgcct ttcaccacct gataacacca tcccaatatt ttttcgcacg tgaccaaggc
     8281 tgaattcctc aatcaggctt tcaacttttt ccctttgttc atgcctggaa agtttactca
     8341 tctccaaaac tgccaacaga ttttcttcaa cggttaattt tctaaatact gaagcctcct
     8401 gggccagata tccaataccc agtttggctc tcttgtacat ggggagtgaa gttatatcct
     8461 gatcttcgag aaaaatcctg ccgtcatttg gtttgatcag gccaacaatc atgtaaaatg
     8521 aagtagtttt tccggcgcca ttagggccaa gcagcccaac aatctccccc ctgtttactt
     8581 ccagggaaac attattcaca acggttcttt tcctgtaaat cttgtaaagg tgatcggcgc
     8641 gtaatttcat ttcacggggc caaatttaat gtctttgatg gtgacttgaa ggaaagtttg
     8701 acccatatat tgattttcat ccaaagaata agccattaaa aagggttggc ctgaagaaat
     8761 ttcgttaaaa tgttctgaaa gattaaaacc gatagcctcc cacttgcgct taccgttttg
     8821 ctgcttcaca aacattttga gatgtttatc tttcagaatt ctgggagtac cggtacacac
     8881 cacatcatca caataaaaaa caggcagcat atttccgggt ccgaaaggcc caatttgacg
     8941 aagaattttg taaagatttg aattcaattc atcaaggggt aaatagtgat cgatgatgat
     9001 tttttgagtg agttgttcta tagtgatggt actgctaact tcagattcaa atcgttgaac
     9061 aaaagattgc acattatcca ggtgcattgt gagtcctgca gcatgggtat gaccaccaaa
     9121 tttcataagc aagtcagagc aattgttgat tgcttcatga atatcaaagc cttccacaga
     9181 acgggcagaa ccggtggctg ttccttccga ctgagtaaga ataatggtag gtcggtaata
     9241 tctttctata catcgggaag ctacaattcc gattacgcct ttatgccaat caggcttaaa
     9301 aagaacagtc gattttgcgt ttagtaaccg atcactttcc tcaatcatcg ccaaagcttc
     9361 ttcagttatg ctggagtcaa attcttttct ttttgagtta tgtgcattta gtgtttcggc
     9421 aagtttcatg gacgaatcca tggagtcact aatcagaaga tttacagaat cccttgcgtg
     9481 atgaattctt cccgctgcat ttacgcgagg cccaattcca aaaaccaaat ccataaccga
     9541 aattttccct gagattccgc tggccttaag cagtgcccgc aaaccgggtc ttggttttga
     9601 ttccagtaat tttattccat gaaaagtcaa aatacgattc tcgccggtaa tcggaacgat
     9661 atccgccgcg atggagatcg caagtaaatc gagataggca aaaagcttct cgtgatctat
     9721 tttgttcctg atgcaaaatc cctggagtaa tttgaatcca actccgcagc ccgtaagttc
     9781 gtcatagggg tagccgcaat cttccctttt gggatcgagg acagcaacag cgggcggtaa
     9841 tgttgagccg ggcttatgat gatcgcacac gataaaatcg actccgttat ttctggcttt
     9901 ttcaattaat tccacggagc gaatgccaca gtcgagacaa attaccaatt taataccact
     9961 atcgattgcg aactgaatgc cctggtcaga tataccatac ccttctttat acctgtcagg
    10021 aatatagaat tcgatagccg aatggaattt tctcaaaaaa ctatacacta atgcaacggc
    10081 cgatgtccca tcaacatcgt aatctccata aaccagaatt ttttgttttg atgaaatagc
    10141 catacaaagc ctctcaacag cgacatccat atccttcatc aagaaagggt caaagaggtt
    10201 ttccagggag ggtctgaaga aagctttcgc tttttcaaaa tcatcaatat tcctctgaat
    10261 taatatggag gctattgcat gatgaacatt cagggttgca gcaaactccc ttactctttt
    10321 ttcgccgtca tccggcaaaa agatccattt cttattcata ctaattaagg aagctttttc
    10381 aaaaataggg ttacaaagaa aaagccccgc tacaacgagg cttttttatt tttttcggat
    10441 gaattttatt ctgacttttt ctctggctga attccttcca gtactttttt cagatcaacg
    10501 cgctgaccat cctcgaaggg aactacccat gcgtctttaa tacccatttc ccgcaaatgt
    10561 tttttcaaag tatctgcagt ccaatagtcg cggaagttgc caaaattaat tctttgagaa
    10621 ccatcctcgg cggtaaatcc ggttatattc ggattacctt caaagaaagc agacagatct
    10681 ttgtttttga aagctcctat ttgaaccttg aatacgaggc cttgatccca attgcctgcg
    10741 ggtggtttag cctccatctc ggcgcgcatt tttctcactg attcttgtgc cgaagctaaa
    10801 tcagcacggg acctattaag ttgatcctga agctcactga tttttgcgtc tttatcagcg
    10861 gtctgggagt ttaaattatt caactgggtg ttcaaactac tcacctgtga tttcaggctt
    10921 tgattttcct cttcaatatt tttcaggctt tccggatttt ttttgtattc ctttgccttt
    10981 tttgcccatg cctttttctc ctgtttggat aattgagcat gtgtggcgtt aaaagaaaaa
    11041 atcaacgcca gacataatac caatgctgaa atagttctca tttctgtatt tttttaatat
    11101 tattcttgat aaaagtagga aaaaggacaa ttaaacacaa aaaaaactag tttttcagac
    11161 tgccaatcat gtctttggga accacccatt tgtcaaactc ttccgcggtt agataaccca
    11221 ggttcagagc ctcctccttg agcgttgttc cattttcata cgccgttttg gcaatctttg
    11281 cggctttctc ataaccgata tgcgtattca gagccgtaac gagcataagc gaattgtcca
    11341 ggtttttctt caaattagct tggttgggct ctattcccac ggcaagatgg tcattaaatg
    11401 agacacatgc atcacccaaa atcctggcgg aagtcagaaa attgtgaatc attacgggtt
    11461 tgaaaacatt caattcaaaa tggccgttca tgccgccggt tgttacagct acatcattac
    11521 caacaacctg ggcacaaacc atggtcaatg attcggcttg tgtaggattc accttgccag
    11581 gcattatgct agaccctggt tcattagctg gaataatgat ttcacctatg ccgcttcttg
    11641 gccccgaagc taacatgcgg atatcgtgcg caattttcat cagactaacg gccacctgtt
    11701 tcagggctcc atgactttct acaattgcat catgactggc cagggcttca aatttatttg
    11761 gggccgttat aaacggaaga cctgtgagat ttgcaatttt ctctgctact ttttctgaat
    11821 aaccctgggg cgtattaata ccagttccta ccgcggtacc gccaagggca agttcagaca
    11881 ggtgttccag tgtattttct agcgctctga ttccgtgatc aagctgggaa acatatccgc
    11941 tgaattcctg acccaatgtc aggggagtgg catccatcag gtgagttcga ccaatcttca
    12001 catcattcat aaatttcttt gacttggcat aaagtgtgtc ccggagtttt tttattccgg
    12061 ggatagtagt ttccaccacc attttgtacg ccgcaatgtg catggcggtc gggaaagtgt
    12121 cattcgagga ttgagatttg ttcacatcat cattaggatg gacagccggg tttccatcac
    12181 ctagtttgtt accagacaaa acatgcgaac ggtttgagat cacttcattc acattcatgt
    12241 tactttgagt gcccgaaccg gtttgccaaa caactaatgg aaactgatca tccagactgc
    12301 aggataatat ttcgtcacag actctcccga ttaattcatt tttttcccta ggtaaaacac
    12361 caagttcaaa gtttgtgatt gcagcggcct ttttcaaata tgcaaaagca taaattattt
    12421 cgatgggcat tttctctttt tcagaaccga ttttaaaatt gtttctggat cgttcagttt
    12481 gcgcccccca gtatttatct gcggggactt ttacttcacc catagtatcc ttttcaatac
    12541 ggtattccat cttgttttaa tttaaaagta aagttagaaa ttattgttaa atgccatgtt
    12601 ccggtatatt aatctattta atatatccac cttttaaagc catgtcccaa agcacaatgc
    12661 caatagacac ggctacattg aagctatgct tggtgccgag ctgtggtatt tccacaattt
    12721 gatccgactc tctaatgatc tccaaatcga caccttcaac ctcattgcca aaaacggcgg
    12781 caattttaga attgctttca atctccagcc ggtgcaaggg gatactttca gtggtttgtt
    12841 caacactaat tattttatat ccctggtttt tcaatttctt tacagcttca acaccttctt
    12901 tttcatagct ccattccaca gtttctgtgg cgcccagtgc tgttttttgg atctctctgt
    12961 gcgggggttt cccggtaatg ccgctaagaa taactttttc aatccggaag gcatcagcgg
    13021 tacgaaaagc ggaacccacg ttgttcaaac tgcgaatatt attgagcact aaaacaaatg
    13081 gaacttttgg tgcacttctg aattcttgcg ttgagagtct tcccagttca tctaatttta
    13141 attttctcat atttgattat tcagaggcaa tattaaattg ctttaacaaa tgaaaaaatc
    13201 aatctttgtg gggataggtt acctgcgaaa gtaagtccca taatgtcaaa aaccaaaacg
    13261 ggtagagaaa ctcccttgat gaagcaatat aaccttatca gggcgaaata tcctggagcg
    13321 ttattgttat tccgggtggg agatttttat gaaacatttg gtgaagatgc aattactgcc
    13381 agcaaaattc tggatattgt cctgaccagg cgcgccaacg gtgctgcctc tcatatcgaa
    13441 ttagccggtt tccctcatca tgcactggat acatatcttc ctaaactggt tagggcagga
    13501 tacagagtag ctatttgcga tcagttggag gatccaaaaa tggtgaaggg aattgtgaag
    13561 cgtggcgtta cagaactggt tacgccgggg ctttccttcc atgataatgt gttggagaaa
    13621 aggcagaata atttcctggc atcagtccat tttggagaaa aaaacctggg aatttcgttt
    13681 ttggatcttt ctacaggtga attcctgact tcatctggaa atgcagaata tgcttcgaag
    13741 cttttgcaga gtttaaatcc ttcagagatt attttctcta aggctaataa aggaaatctt
    13801 aaagaaatat tcggagataa gtataattac tatcatctcg aagattgggt ttttcagtac
    13861 gattatactt atgaaaaact aaccaatcat ttcaaaacta cgacgcttaa aggatttgga
    13921 attgaatctt ttccggaatc tattatcagc gcaggtgcag ttttatacta cctggaagag
    13981 actgaacata atgagatcag tcatatttct tctgtttcaa gaattgaaga agaaaaatat
    14041 gtttggcttg acaagtttac tatccgaaac cttgagttgg tttacccgca acaggaagga
    14101 ggcattccac tcatccaggt tctggataac accgtgactc caatgggcag ccggttgctg
    14161 aagaaatgga tcgtgcttcc ccttaaagaa aaagatctca tagaggaacg gcttcgaatc
    14221 gttgaaagtt tgatcaatga ggaaacagga agtgaagaaa ttcttaaaaa tctgaggcag
    14281 atttctgaca ttgaaagatt agtaggaaaa gtttctgccg gtcggataaa tccgagggaa
    14341 ttgttgcaat taaaacgcgc tcttaagcac acattgccga taaaggagat tctaaaaaga
    14401 tcagccaata aggacttgca gaaacttggg gagttgataa atccctgtga ttttgtaatt
    14461 cagaaaattg aatctgaatt gtcagatgat ccaccaatgt tattacactt gggaaaagtc
    14521 atgaagaaag gagtaaatgc cgaactggat gaattaagag aaatcgccta ttcaggaaaa
    14581 gaagttttgg ttcagattca acaacgggaa ataaaacaaa ccggaatttc ttcattaaaa
    14641 gtagcttata ataaggtctt cggatattac ctggaagtta ccaacgtgca caaggataaa
    14701 gtgccttcta actggataag aaaacaaact ctggttaatg ccgaaagata tatcacggag
    14761 gaattaaagg tttatgaaga taaaattctc accgcggaag aaaaaataac ttccattgaa
    14821 caaaaactat attttgattt ggttcgacag gtttctgatt tcattgttca gattcaggag
    14881 aatgcaagaa ttctggcaaa acttgactgc ctcctttctt ttgctgagac agcaaccaaa
    14941 agtaaatatt gtaagccgga aattgacgac tctttggaat tggaaataat agacggccgg
    15001 cacgccgtaa ttgaaaagca attacccacg ggagaggagt atgtgccaaa caacttatac
    15061 cttgataacc attctgatca gattttgatt atcaccggcc cgaacatggc cggaaaatcc
    15121 gctttactcc ggcaaacggc attaattgtc ttaatggctc agatgggatc ttttgttcct
    15181 gcatcaaccg caagaatcgg tttgatagac aaagtgttta cgcgggtggg agcttcagat
    15241 aatttatcac gtggtgaatc aaccttcatg gtggagatga tcgaaacggc aagtattttg
    15301 aataatatca gcgaaaggag tttgattctt atggatgaaa tcggaagagg gacaagtacg
    15361 tatgacggtg ttagcattgc ctggagtatt gtcgaatatc tgcacaaaat aaccacaaga
    15421 cccaaaacac ttttcgcgac acattatcat gaattgaatc aactttcgga agatcttccc
    15481 cggataaaaa attttaacgt ttcagtgaag gaagttggag accggataat tttcctcagg
    15541 aaactgaaac caggcgggag cgaacatagt tttggaattc atgttgctca gatggcagga
    15601 atgccgaatt ccattgtatt gcgtgccgat gagattttgc atttcctgga aaaggataag
    15661 aaaaaaagcc aggagaaaaa aatgcgggaa gtgccacggg atgatttcca gctgatgctt
    15721 ttcgacaatg atcccaaaat caaacaggtg aaggaaatgc tggatgcact tgatataaat
    15781 actatgtcac cagttgaggc tttattaaag ttgaatgagt ttaaagggtt attggggaaa
    15841 tagtctgata aatcattcta tggatttaat acgtcttcgt catatctgat ggaatcacaa
    15901 taataaaatc tgcctttccc aggttgtcat tgtccaggta ggaaatatcc ttttgttcca
    15961 acaattgaat agtgatcact tttagatctg gcctggattg ctttatgtac cacacaatgc
    16021 cttcatgatt ttgtgaatgg taagaaccat tgaaatgcag aatcttatta gataaggttt
    16081 tgttttgcag gatgaagtga gccatggtag catctttcac cgcctgcgca aaggcaatat
    16141 tattaggatc tccgtcacca tggctcccca tcatcgcgat catgcttttg tatccgggca
    16201 actcaaggtc gatttttatt ggcagcggag caatccattg ctttgcttcc gaagttaagg
    16261 aatccagcga ggacaaaccc tgacgataaa caaggtttgc ataccttcgt ggaatattgg
    16321 aagcgataac aggcaactgg ttttttcttg caaactccac aagtggtttg taatctgttt
    16381 tataattatt ccagactttg gcttctttta aaagatgatc ttccttgata acattgtcca
    16441 gatattcagt cagaacaatc tgatcatcgg cttcaaacat ttccattgct agcaccaatt
    16501 ctgaattttc cgcgtaaata ctttcagtga ttttcagttc cagccaatga gcaattgagt
    16561 tgttatggag ttcccccacg aatacaattc cggcttcggc tgcaccatca atcattttgg
    16621 aatagtccga aggctttcct tctgagctat atattttata ggctggaaga tcctgggcat
    16681 ttacccgggt aagtaccacc accaaaagga tcatcaggga attgagtctc ataatatttc
    16741 tatttaggct gaatatagta acaaatgaga aggtaccaat cgccggtcct ggataaacac
    16801 ctgggatcgt ggtgttggca ctttacatgc ttgctggatt atccttttaa tttccttcca
    16861 aatattaggt gttgtaatga gaaatatcaa ctgttgcctt gataagggtc atagtaaacc
    16921 ggtcatttca gggatagatt ggcaccataa aagaataaga ttatgaagaa tcttgtaatt
    16981 cttggtgccg gcactgccgg aacaataatg gccaataagc tttacaaagt tttgaataaa
    17041 gatgagtgga aaatcaccat cgtggatcgt gataatttac attattacca gccgggattt
    17101 cttttcatcc ctttcggcgt ttataaaccg gaggaggtaa taaaaccgaa agcggacttt
    17161 ttaccggacg gtatcacttt tattataaat aatgtaaagc gagttaagcc ggaaaaaaat
    17221 gaagtaatct ttgaagatgg caaaatcata gaatatgatc tcctgataat tgccaccggt
    17281 acaaccccag tacctgaaga aacggatgga ttaaaaggta agctttggta caaggatatt
    17341 ttcgattttt atacctacgt gggctcagtt aacctggcta agaaactgga aacatgggag
    17401 ggaggaaaat tggtaatcaa cctggctgaa tccgtgatca aatgtccggt tgctccactc
    17461 gaatttgcat tcctggccga cgcttatttc attgaaaaag gaataagaga cagagtagaa
    17521 atcttttatg tgactccgct ttccggggca tttacaaaac cgaaagccac cgagatgctc
    17581 ggtcagttaa tggaagagaa aaacataaaa gtgattcctg acttttactt aatgcgggtg
    17641 gatgaggata gaaaagtaat tatttcttat gatgaaaagg aagtcccttt tgatttgctg
    17701 gtttccatcc cagtgaataa aggtgacgag atgattgcca acagcaattt gggagatgaa
    17761 gacggtttga atttcgttcc aactgatatc catactcttc agtctaaaca atttgacaat
    17821 gtattcgtga ttggtgactc aaccaacatc ccggcttcga aagcaggatc agtggcacac
    17881 tttgaagctg atgttctaac agaaaacata atcagtttca tcaacaataa accccttgaa
    17941 gcgaaatttg acggacacgc aaactgtttc atcgaaaccg gatttggcaa agcgactttg
    18001 atcgatttca attatgacac cgaacctctg cccggaaaat ttcctttccc gattatcggc
    18061 ccgatgggtt tgctgaaagt aacccgcagt aatcaccttg gtaaattatt atttaaatgg
    18121 gtctattggc atttgttatt gaccgggaaa aaattgccgg tctccacaga aatgtcgatg
    18181 gctgggaaag taaattctta aaacttcaaa taaaaaatta tggaaacttt aacactagac
    18241 agacaagaaa ttgaaaggaa tgtggaagga tatctgaaag atgcttctca atggaatgaa
    18301 gaattggcaa aagaaattgc agcggagtat aattttattc ttacggagaa acatttcgaa
    18361 gtactgcgat ggctgcgcgg gaaacaaagc gaaggtgtta ccctcagcat tcgtaaagtt
    18421 ggagcttccg ggcttgttga tataaaacaa ttctatcaac tctttccggg tggacctttg
    18481 aaagtctcaa gtaaaatcgc aggcatacct aaacctgtaa gttgtatttg aatatataat
    18541 taatgaaatt atcatggtag aaaccttgga aaaaacaccg gtaactgcaa cagttgaagt
    18601 tccggttaaa caagaaagag atacagttaa gaaagtattg atcatctgct caaaaggtac
    18661 actggaggat gtgtacgctg ccttaattat ggctaacggc gcattgatgg atggcagaga
    18721 tgcaaaactc ttctttactt ttttcggctt aaacgcgatt acaaagaaaa atgcggatca
    18781 cctgcatact gcgacagtag gtaatccggc ctttatgccc gcgatgccga ctttggttgc
    18841 cggccttccc ggctttgaag cctttgcatc ttatatgatg aaaaaagaaa tggacaaact
    18901 ggacatcccg cctgttacag aattttttca aatgattgat gcggccgggg gtgagattta
    18961 cgcctgcaag ttagccgcgg agatgttcaa gttgaacaag gaagattttc tcgatgaggt
    19021 taaagatatt atcaccgtag gagatatgta cgccctggca gatggggatg gatcacattt
    19081 aattttcatt tgacgatgtg ggcttggaaa taggaaagac tttgaacaaa acaaatggcc
    19141 cagccgtagc ttcttaaaat gattcgcaga aatcaggaag aaatcacaga gtgagagtca
    19201 gagtgggatc tggaaactca cactcactaa gtacccgggg cgggacttga acccgcacgg
    19261 cattttactg ccacaggatt ttaagtcctg cgtgtctacc aattccacca cccgggctaa
    19321 ttaaattctc aaggagggtt atcaaaaaat tgaaaataaa aatgagcgaa agacgggatt
    19381 cgaacccgcg accccgacct tggcaaggtc gtgctctacc aactgagcta ctttcgcttt
    19441 taacctaaaa tgggattgca aatttacgta agcacccaaa ataaacaatg gttttaaatc
    19501 aaaagtcaat aatttgaatc cattccaact taatctaccc aaaagatttt tctttgtagt
    19561 tcgatattat ttcctcaaat tactcacctg tttgcactaa acccttatca accatgaaac
    19621 ttaatgtata cgttaccatt gcgcttttac ccctttcatt tgccttaaat gctcagagtt
    19681 ttgtaactcc tttggatgga acaagtctgg gaatgtctaa atctaaccgg gtaaccacaa
    19741 aagatgggaa ggtgcatgaa ggtaaaatcg ttttttctac ctatgccgcc ggtcagatat
    19801 ccggatttac cctaaaaaca gaaaccgaaa aattaaaatt taaggcagaa gaaattgctc
    19861 tacttgaatc tgaacccggg aagcttcaaa aattctcaat ggccactgaa ggcaggaccg
    19921 tttattccgc attagccaaa gattacagtt atatccagga acttaaatat gtaacttttg
    19981 aaccggtaga gattaaagaa aaagtgaaat tgatgcaatt attgaacccc ggatttgatg
    20041 agtatattaa agtttatctt gatccaacgg ctaaggaaaa ccagtggacc gggcaagcca
    20101 attcttattt tgtgaagaaa ggtgacgaag ccatctacct gaaaaaaggt aaatatgatg
    20161 acatgttttg ggagttgttc agcggatgcg aaaaattcaa agagaatttt gaaggtgaac
    20221 taaagtttta cgactttccc agtcatatcg cattatacaa tgagagttgc ccaaaggaat
    20281 agacaacatt cagattttga aacttcaatg tgcaattgat taatcagaac tagccaattg
    20341 cacattgata attttcccgt gaaaattgaa ctctaattca atgcgaaatt aatcggaata
    20401 acaacccttg tcttcactgc atttccgcgt tgttttcctg gattccaggc tggactgttc
    20461 ttaacgactc gtacagcttc ttcatccaat ccagctccta ttcctcgaat agcctctact
    20521 tcactaatgt ttcctttttt atcaacaaca aattggacaa acactttccc ttcaacaccc
    20581 atctttctgg ccgagggact ataatgtaaa ttttttctta gatattcgtg aaatgcatac
    20641 actccgcctg ggaagcttgg catctcttca acaatagtga aaatttcttc aacttcttct
    20701 tcctggggag cggcttccaa aataatttct tgcactaccg attcctctgt gatctcaata
    20761 tccaggtcta tgggaatttc ctcgatcacc tcatcatccg gtatctccac aattattggt
    20821 tgctgaattt taggtggggg tggcggcggt tgctcagttg gtgggatttc gagcatctcc
    20881 tcaaaatcat cgttcactgt gcccagcgat accaactgac catcatcata aaatttccac
    20941 tcgaaagcag ttattacagc aatcagcgtt attaccaatc cgatattgaa gaacaacccg
    21001 cgcagtttcg ttaattcgta agctttattt tttttgagtt ccatactaat aatctttccc
    21061 ttatttacct caattttgat aacgcaaaaa aagtcctttt gtttcacttt cggacatccc
    21121 ccacgttatg ttattattaa caaaataaac acaaaggatg agccaaacag ggcatttttt
    21181 aagtaggaca tcataatcct caaaatcccc ctttaagcct ccattttaac cacacatcga
    21241 aatgaaaaat cggccatttt gtctgcttgc aggtattaga tttcataatt caacaaaaac
    21301 aagcgattgt tgatactaaa agtagtatca gttagaattt tcatttctta aatacgccaa
    21361 tttatcaaag cgatattaaa cctgttagag gtcatgccaa ctatttattt ttttttgcaa
    21421 aattaagcct gctgacgtaa aacaatattg gaaaaaattc accaaagaca attcgcattt
    21481 taattctaat caatttttgc taaattcctc actcgcatca aactcccttt ctcatgaaaa
    21541 aattattaat cctgctcata atatccggct tatttattat tgatgtggct ggtcagggaa
    21601 acgatcctta caatgaaaaa ttttacaata cgctcgagtg gcgaaatatc ggaccagaaa
    21661 gaggaggaag gtctttggga tgcagcggaa gcccgggtag gccgaatgag tattattttg
    21721 gcgctacagg cggtggttta tggaaaactg tggatggtgg tcaaaactgg tttccggtaa
    21781 ccgacggcca aatcacctgc tcatcaattg gtgctgtggc ggttgctgaa accaacccgg
    21841 atgtggttta tattggaggt ggtgagattc agctccgggg tagtattact cagggtgatg
    21901 gtgtttataa aacagttgat ggtggtaaga cctggcggca tctcggttta gcggagacgc
    21961 aggctatcag caggataaga attcatccca caaatcctga cattgttttt gtcgccgccc
    22021 ttggccatcc ttatggcgac aaccaggagc ggggcgtatt tcgctccaca gatggtggaa
    22081 atacttggca aaaagtctta ttcgtatcag agaaggccgg cgctgctgat ttgataattg
    22141 acagaaccaa cccaaaagtt atttatgctt ctacctggca ggtttaccgc aaagcatgga
    22201 aaatgtgggg gggcggtccc gatagtaaac tttttaagtc agtagatggc ggagataatt
    22261 ggatagaact aactaaaaat ccaggtatgc ccgagggtcc gattgggaaa ataggagtaa
    22321 ccgtttctcc cgcggatcct aatcgcgtat gggctattgt tgaagcaaat gagggcggtg
    22381 tttttcgttc ggatgatggc ggatggacct ggcaaaagac aaacgatgaa aggaaattac
    22441 gacaacgtgc attttactac tcaaggattt atgcagatcc ctgggatagg gaagtagttt
    22501 atggattgaa tacaggattt tacaaatcta ctgacggtgg aaaaactttt gatgttacaa
    22561 tacgcgtacc gcatggggat aatcatgatt tatggatcga cccaaacaat cctgatcgca
    22621 tgatcaattc taatgatggt ggtggcaatg tttcgattaa tggcggtaga acctggacag
    22681 aacaggatta tgttactact caattgtatc atgtgatgac caccagcgat gttccctacc
    22741 atgttgcagg tgcacaacaa gataatagca cgattgctat acccagtgat gggtggggta
    22801 atatgcaggc acgtggaccg aatcatggct ggtactatgc cgttggcggt ggcgagagcg
    22861 gctggatttc gcagcaccct gtgaataaag atatttttta cgcagggagt cagggggcat
    22921 tgcttacgcg gtacgacaga agcaatggtc agataagaga tatacaagtt taccccaggt
    22981 tcttctccgg tgaacctgcc agcgctttgc ctgaacgatg gcaatggaca ttccccattg
    23041 tattttcccg ccaggaccca aacatcatgt atacctgctc tcagcatgta tggaaaacca
    23101 tggatgacgg gcaaagctgg ataaagatta gccccgactt aacttacgca gatcctgaaa
    23161 cattggggcc aaccgggggt attattacga tggatatgaa tggcccggag atttatgcta
    23221 ccgtatttgc actggaacca tcacaccatg acataaatac cctgtgggtt ggctctgatg
    23281 atggtaaaat tcacattact cgtgatggag gtaaaagatg gagtgacatt acaccgaaag
    23341 atatgccaaa attttcaaga gtcagtataa ttgatgagtc tgaacatagc cccggaaccg
    23401 cctatgttgc agcaaatagg taccaggtag atgatcgtgc gccttatgtc tggcgtacta
    23461 tagactatgg taaaacatgg acaaaaataa cgaacggaat aaaagacggt cactttgcca
    23521 gggcagtacg cgaggaccat gtgcagccag gcttgctgtt tctcggaaca gaacatggcg
    23581 cctacgtgag ttttgatgca ggagagaact ggaaaccttt acaactgaat ttgccggata
    23641 cgccaatcag ggatttggtg gttaaagaca atgatgtagt cctgggaact catggccggg
    23701 gtttttggat actcgatgac attcaacctt tgcgggaact gggaccacaa ctagctcaaa
    23761 agaatgctca tctttataag ccggcagatc ctatccgcgg gatttataat gccaatttcc
    23821 aatactacct gaaaaatcca gtggatacaa ttaaagttga aatactggat gcacaagatc
    23881 aggtaatcag aacttttatt ggcactgaaa cttctaaccg ggttgatccc aatacaccct
    23941 ggtgggcagg aggctccggc agcccagcca ctgccaaagg gttaaatgca tttgaatggg
    24001 atctaactta tgaaggagcg acaatttttg atgggatgat catctggagc gcaagaccac
    24061 agcgcgggcc taaggcgcct ttgggcgaat acaaggttcg acttacttcc ggaaactatt
    24121 cggaaaccag gaatttcaca atcaaaataa acccgaacct gaaagggata acacccgagg
    24181 atcagaaatt acaatttgac ctggcaatga agattagaaa taaaactacc gctgctaatg
    24241 aagctgtgat ctcaattcga aaaatacgta aacaaattga agacagaatt gcaaactcag
    24301 gtgatgagca actaaatacc agcgcaaggg cattcattag taaaattact actgtggaag
    24361 aagaccttta ccaggttaag aatcaaagta accaggatcc gctgaacttt ccaattaagc
    24421 tgaataacag gttagcttca ttgcgaagaa gcgtagaaac cggagatgcc aaacctacga
    24481 atggcgccta caaggttttt gaagaattat caaaagaact tgaaggacac ctgacgaaat
    24541 tggatcaatt aattaagaat gatttatcgg tactaaatca atcttttcag agtaaaaatt
    24601 tgaaacctgt aacagtaggt aaggattaaa agagattggg taactggaat ttcctatatc
    24661 attataacac aattaattac taaataaata ctattttggt gcttatttag tataaagtgt
    24721 ataaaaacaa ctcgaagatt ttaattcttg ccgccgcgtt tctttgctta acctgtggaa
    24781 attaccaatc agaaacagaa cgatccaggg agattgtcaa tgaattagct gacgaatata
    24841 tagagagcta ctttgatgcc tatccattta tggcactatt aagcaatgcc cctgatcttc
    24901 atccggaaaa acttgcggat aattccaggg aaggaattgc acaactggag aaaaaacagt
    24961 tacagctttt gacaaggctg aacgcaatta atccggaatt gatggatagt gagacgcaaa
    25021 atacttattt cattctgaaa aaccttcttg aaaattccct tgccatttgc acctgccgca
    25081 tggaactttg gaatgtaagt cccacctgga cgggctggca gtctctcttt tcactcatgg
    25141 caagtttgct gccaattgaa actcctgaac aaaaaaataa agccataaaa cgcctgtcac
    25201 agattcctgt ctacctggaa atggaaatca ggaatttgag ggcaggcatt caattgaatt
    25261 attcggctcc tgctaacaac gtgcgggttg taatcgagca gatgaatgcg ttgattgcgg
    25321 gaggaccgga gaatttgcca tacacaaata ttgccggaga aagcgatcta aacttcaaag
    25381 aaaaattgga aagcctggcc tcacttaaaa taattcccgc aatagaaaat taccgcgatt
    25441 ttttgcaaga tgaatacctc attaaaacta gaacttcttt tggtgttaat gaaaacaggg
    25501 atggcattga atgctataag gcttcattga agtattatac aacaacaaac ttttctccga
    25561 aagaaatcca tgaacttggc ctaatgctgg ttgcaggaat tctggccgaa gtaaaaaact
    25621 taggacagaa agtaatggga attcgggatg tccggaaaat ttttttccta atgaattccg
    25681 aaccttcatt cagatttaac aatagagaag aaatacttat taatgctgaa cggtcaattg
    25741 aatctgtgcg ggcaaagttg caagattggt tcaatgttgt tcctgaaaca aaaattaaag
    25801 tgcagccctt ccctgatttc caggaaaaat ttgcacccgc gggcgaagcg gttgcagctc
    25861 cggaggaagg aggaacggga ttattctata taaatactta caatgctaat gaacaaagca
    25921 gggctggttt gcaatccatt gtttttcatg agtcaatccc aggacatttg tttcaatttg
    25981 cggtcgccgc aggcaaaaaa tctcacccca tttgtaagta tttctacttg tcgggccatg
    26041 gtgaaggctg ggcgctttat gcggaacgac tcgctgatga aatgaatctg tattcctctg
    26101 atgtggaaag actgggaatg ctctcaaatc agttactccg cgcggcaagg ctcgtggtgg
    26161 atacggggat acatgctttt tcgtggtcac gcgatgaatc gatagattat ctcatgagta
    26221 atgccggttt gcaaaggtca gccgcggaag ccgaggttga caggtatata gcagttcccg
    26281 gacaggctac agcttacatg gttggttacc tggaaatcat aaacttaagg gatgcggcag
    26341 agaaaaagtt ggggaaagcg tttgtactca aagaatttca tgatttaatg ctcgaagaag
    26401 gaaccctgcc gctatggctt ctcagggaaa aggctgaatc ctggattcag agaaacattc
    26461 aaaaataatt tccataaatt tttttcatac ctgaaaatta cccaaatagt cgcccgccct
    26521 ggtgattttt ctttcaaata aaatatatta caggtcacaa catgagccgg ggaaaaagaa
    26581 tagccaaaat aaatatgtcc ggggaggagt tccgcaaatt aggatatgaa ctaacggaca
    26641 aaattgcgaa gctacttgat gagattgata agaaaaaagt taccagtggt ctttccccgg
    26701 ttaaaatcag ggaactgctg aataccgata tagcattaaa agattcgggg agcaagagtg
    26761 aagaaattat tgaccgggcc gccgccttgt tgctggagca ctctctgttt aatgggcatc
    26821 ctcggttttg gggatatata acttcatctc ctgcaccgct gggaattctt gctgattata
    26881 tggcttctgc cataaatcct aatgttggtg gatggacctt atcgccggtg gctactgaaa
    26941 tggaagtaca aaccattaag tggatcggtg agctgattaa ttataaggcc gacgggggca
    27001 ttttagtaag cggtggaaac atggcgaact ttgtaggatt tctggccgga acccgggcaa
    27061 agctgggaca agatataagg agattgggcc tggcaggaag aacaccaaag atttattgtt
    27121 ccgcggaaac tcacacctgg atccaaaagg cagcggactt atttggattc ggaactgacg
    27181 caaccgtgtg gattcccacg aatgagaatc agcaaatgaa cgcagatgaa ttacagaaga
    27241 ggattgaaaa agataagaaa catggcagtg aacccatcat ggttattgga accgcgggaa
    27301 gtgtaagcac gggggcaatc gatcctctgt acgaaattgg agcaatttgc cgggaacata
    27361 atctttggta ccatattgat ggtgcttatg gaggatttgc agcttgttta cccgaaagat
    27421 ttccggaact tgaagccttc caggatgcag attccatagc tgttgatccc cataaatggc
    27481 tttatgctcc tttggaagcc ggctgcactt tggtaaaata tgcgcatact ttacccgaca
    27541 cgttttccta tcatccgcca tattaccact ttggagtgga ggcggtaaat tttgtcgaca
    27601 atggctttca aaatagccgt ggcttcaggg cattgaaagt ctggatgatg ctgcagcagg
    27661 ttggtagcgg gggatataaa gaaatgattg cggaagactg cgacctggct aaacatcttt
    27721 atgagattct tccgcggtat aaagaattgg aaagattcac ttataacctg agtataacta
    27781 cgtttcgata tattcctgaa gaattaagaa aaaaagccaa agaggaaagt acctcaaaat
    27841 atctagatga cctaaataaa aacattcagg ctaaacttga atcaggcggt gatttctttg
    27901 ttagcaatgc ggtaatcaat ggcatatatg tattacgaat gtgtattgtc aacttcagaa
    27961 cttccaaatt cgatgtggaa gctttaccgg aaagagttat tgaattggga agggaattgc
    28021 attaacaaaa aagcaa
//
LOCUS       DASWHX010000079         4117 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_3380367, whole genome shotgun sequence.
ACCESSION   DASWHX010000079 DASWHX010000000
VERSION     DASWHX010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 4117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4117
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3380367"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..758)
                     /locus_tag="VGA21_05700"
     CDS             complement(<1..758)
                     /locus_tag="VGA21_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015331259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX9650033.1"
                     /translation="MKKCILFFMTLPALAIGGQKTAEEVIQSLDKSTSKYEKLAFQIW
                     NNAEVGYLETKSSEALQNELKNAGFAVMAGVAEIPTAFVASYGEGSPVIGILAEFDAL
                     PGLSQKAVPEKSPIIEGNPGHACGHHLFGTASTAAGISIKNWMVANNIKGTIRVFGTP
                     AEEGGAGKVYMVRAGLFNDVDAVLHWHPGSANSASASSSLANKSAKFRFYGLATHASA
                     SPHRGRSALDGVEAMNMMVNMLREHVPMESRIHY"
     gene            complement(859..1719)
                     /locus_tag="VGA21_05705"
     CDS             complement(859..1719)
                     /locus_tag="VGA21_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014796981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HEX9650034.1"
                     /translation="MKQLKIIQQITNRESKSLDKYFTDISKVDLIDSDEEINLASRIR
                     EGDTLALEKLIKSNLRFVVSVAKQYQSNHMTLGDLINEGNVGLIKAATRFDETRGFKF
                     ISYAVWWIRQSIMQALAEQSRMVRLPLNRISSINKISKASSQLEQVLERDPTAEELAE
                     VLDSIPDEIRNNMMISKRQISVDAPFVTGEENGLLEVIINTEIENPDSGLMRESLRQE
                     IQHVLATLTERESKVLTMFYGLNGKSSLSLQEIADTFDLTRERVRQIKEKATRKLQKS
                     SRSKILRNYL"
     gene            2073..2969
                     /locus_tag="VGA21_05710"
     CDS             2073..2969
                     /locus_tag="VGA21_05710"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9650035.1"
                     /translation="MNKIYDYPNLSFQKDRYRMTVKTNSPREYALVTGGSNGLGRSIS
                     HQLAVRGYGIILVAFDDSQLFETAADISAKTGVDVKVIGTDLNLPGSCEEIASQVKAF
                     QINLVILVNNVGLGDVSFFHANTVSFNQKIVRLNCSVMVELTQLLLPELKKSKNPYIM
                     NISSLAGLFPVPYKAVYSASKHFVKAFSFSLREELASDNVTVTVVFPGSILTNPDVIR
                     RVNSFGRLARICSYKPDEIADLSVRSLFRKKREVYPGLINRLYILIRYIIGYRITMNL
                     VKKRMHKEALWDLNNKIEIKVK"
     gene            complement(2984..>4117)
                     /locus_tag="VGA21_05715"
     CDS             complement(2984..>4117)
                     /locus_tag="VGA21_05715"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008626056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HEX9650036.1"
                     /translation="IARGAMNKSNVMIKPKMPTFSRDYRDFTIALNKRVNNYFKINKI
                     SRHGNREMIIKTVFMFGLYFVPYALIITGVLTSMPALILAVMVMSLGVSGIGLSVMHD
                     ANHGAYSKSPGINNFVGYSLNLVGANSFNWKIQHNLLHHTYTNVYEEDEDISPKGVLR
                     LSPHSPWKKIHKFQFIYAWFLYGLMTLSWLFFKDFERIVKYYKNGLINKQKANVTTEW
                     IILIITKAIFIGYIFFIPWIFTPLVWWQILIGITMMHYAAGFFLAIIFQPAHVITGTE
                     FPLPDEENVLKYNWTVHQLHTTTNFGNKSKWFSWFVGGLNFQIEHHLFPNICHVHYRK
                     ISEIVKSTTEEFGLPYKNARTFIGALYNHLKLLKQLGLKPSHN"
BASE COUNT         1210 a          824 c          759 g         1324 t
ORIGIN      
        1 atataatgaa ttctcgattc catcgggaca tgttcccgaa gcatgttcac catcatattc
       61 attgcctcaa ccccatcaag tgctgatctt ccccgatgcg gagaagctga ggcatgagtt
      121 gccagcccat agaacctgaa ttttgctgat ttgttggcca gggaagaact tgcacttgca
      181 ctattggctg atcccggatg ccagtgaagt actgcatcca catcattaaa caaaccggca
      241 cgaaccatat acactttccc cgctcccccc tcttctgccg gagttccaaa tacccggatt
      301 gtacctttta tattatttgc aaccatccaa tttttaatcg aaatgccggc agctgtactg
      361 gcggttccaa acaaatggtg tccgcaagca tgcccgggat ttccttctat aatgggtgac
      421 ttttcaggaa ctgccttttg ggataagccg ggcagagcat cgaattctgc aagtatgcct
      481 atcactggcg aaccttcacc ataggaagct acaaacgcag tcgggatttc tgcaactcct
      541 gccattaccg caaatccggc attctttaac tcgttttgca gtgcttcaga acttttagtt
      601 tccagatagc ctacttctgc attattccag atttgaaaag caagtttttc atacttcgaa
      661 gtgcttttgt caagagattg aattacttct tcagcagttt tctggcctcc tattgccaat
      721 gcgggcaagg tcataaaaaa aaggatgcac tttttcatgg tgtatttatt ttatgtgaga
      781 aaaacaaatt tataaattgt aagagaagta aaaagggttt ccagtggtat gtgttgcaat
      841 accatttggc ccgttggatt ataaatagtt tcgaagaatt ttacttcggg aacttttctg
      901 aagttttctt gttgcttttt cctttatctg acgaactcgt tccctggtca ggtcaaatgt
      961 gtccgcaatt tcctggagac tcaaacttga cttgccattg agaccgtaaa acatggttag
     1021 caccttgctt tctctttcag taagagtagc aagtacatgc tgaatttcct gtcgcagtga
     1081 ttcccgcatt aatcccgaat caggattttc aatctccgta ttaatgatga cctccagcaa
     1141 accattttct tctcctgtta caaatggagc atccacagaa atttgcctct ttgagatcat
     1201 catgttattc ctgatctcgt ctggaataga gtccaacact tccgctagtt cttcagcagt
     1261 gggatctcgc tccaatactt gttctaactg agatgaagcc ttcgagattt tgtttatgct
     1321 tgagattctg tttaggggaa gtcgtaccat tctgctttgt tccgccagtg cctgcataat
     1381 ggattgtcga atccaccaga ctgcataaga gataaattta aatcctcgtg tttcatcaaa
     1441 tctggtagcg gcctttatta atcccacatt tccttcattt atcaaatccc cgagagtcat
     1501 atggttactt tgatattgct ttgccacaga aactacaaac cttaagtttg atttaattaa
     1561 tttctcaagg gcaagcgtat caccttctct tattctggat gctaaattaa tttcttcatc
     1621 agagtctatt aagtccactt tacttatgtc tgtaaagtat ttatccaaag acttactttc
     1681 tctgttagtg atctgttgga ttattttgag ttgcttcata gtttagggca aagcatacat
     1741 cggaaggctt tctttttcag gttgatacat ttagaaattg agatgttaat ctgactacag
     1801 ttttggattg aaatcctcaa gagcagtgaa aaataaaatc ctcaattttg caaaatgtaa
     1861 gttaggccaa tttaatcgga aacccatcat aattgcatac ttaaatggtt acccaagagt
     1921 atgttggttc taataaaatc catgtcaggt attcagctgg ttattctatc tatttgtcca
     1981 ccttctattt gtccaccttc caaaaatcag aaggtgggaa ataaaataac atcttttttt
     2041 tgtcacgttc gtttgatatt atcctaattt ggatgaataa aatttatgat taccctaatt
     2101 tatcatttca gaaagaccgt tatagaatga cagttaagac caattctccc cgcgaatatg
     2161 ccctggtaac aggaggaagt aatggtttag gcaggagcat ctcccatcag ctggctgtca
     2221 gagggtacgg aattattctg gttgcctttg atgattccca actttttgaa accgccgcgg
     2281 atataagtgc aaaaaccggc gtagacgtta aagttattgg tactgatttg aatctgccgg
     2341 gaagctgtga agaaattgct tcacaagtaa aagcatttca aatcaatttg gttattctcg
     2401 ttaataatgt tggattaggt gatgtttcat tttttcacgc gaataccgtt tcattcaatc
     2461 aaaaaattgt gcgattgaac tgttcggtaa tggtagaatt aactcaatta cttctaccgg
     2521 aactaaagaa gagcaagaat ccatacatca tgaatatttc cagcctggca ggcttatttc
     2581 ccgttccata caaggcggtt tattcagcct cgaagcattt tgtgaaagct ttctcctttt
     2641 ctcttcggga agaacttgca agcgataatg taaccgttac agtggttttc ccaggctcaa
     2701 tcctcaccaa ccccgatgtt atccgcaggg taaattcctt tggcagattg gcaaggattt
     2761 gttcttacaa acctgatgaa attgccgact taagtgttcg atctttgttt cgtaaaaagc
     2821 gggaagttta tccgggtctg attaacagac tatatatatt aataagatat atcattggat
     2881 accggattac catgaacctg gttaagaaaa ggatgcacaa agaagctttg tgggatctca
     2941 acaataaaat tgaaattaaa gtaaaataaa ggcgatgtca accttaatta tgagacggct
     3001 ttaatccaag ttgcttcaac aacttcaggt gattataaag ggctccgata aaagtccgcg
     3061 cattcttgta cggaagtcca aattcttcag tcgttgattt aacaatttct gaaatcttgc
     3121 gataatgaac atgacaaata ttaggaaata agtgatgttc aatttgaaaa ttcagcccac
     3181 caacaaacca ggaaaaccat ttgcttttgt ttccaaaatt agttgttgta tgaagttggt
     3241 gtacagtcca attatacttc aatacatttt cttcatcagg taaaggaaat tcagttccgg
     3301 taattacatg ggcgggttga aaaattatcg caaggaaaaa cccggccgca tagtgcatca
     3361 tagttatacc aataagaatt tgccaccaga ccagaggtgt aaatatccat ggaatgaaaa
     3421 atatatatcc tataaaaatt gccttagtga ttatcagaat tatccattct gtggtaacgt
     3481 tggctttttg cttatttatt aaaccgtttt tatagtattt cacaattcgc tcaaagtctt
     3541 taaaaaacaa ccatgacaaa gtcattagcc cataaagaaa ccaggcataa atgaattgaa
     3601 atttatgaat ctttttccag ggagaatggg gtgataatct cagtacaccc tttggactta
     3661 tgtcttcatc ttcctcatat acattagtgt aagtatgatg aagaaggtta tgttgaatct
     3721 tccaattaaa cgagttagct ccaactaaat ttaaagaata gccaacaaaa ttattaattc
     3781 cgggactttt ggaatatgct ccatggttag catcatgcat cacagacagt ccaatacctg
     3841 aaactcccaa actcattacc attactgcta atataagcgc cggcattgag gttaaaaccc
     3901 cggtaattat taatgcatac gggacaaaat agagtccaaa catgaaaacg gttttgataa
     3961 tcatttccct gtttccatgc cggctaatct tgttgatctt aaagtagtta ttaacccttt
     4021 tattaagggc aatagtaaaa tccctgtagt ccctggaaaa ggttggcatt tttggtttaa
     4081 tcattacatt tgatttattc attgctcctc ttgctat
//
LOCUS       DASWHX010000080        10655 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_1168595, whole genome shotgun sequence.
ACCESSION   DASWHX010000080 DASWHX010000000
VERSION     DASWHX010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 10655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10655
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1168595"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            872..1336
                     /locus_tag="VGA21_05720"
     CDS             872..1336
                     /locus_tag="VGA21_05720"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEX9650037.1"
                     /translation="MKQRIYIDTSVFGGYFDEEFSEHTIPLFDRIKNGELILLYSTVT
                     QDELENAPEKVKELVKGLKADFTEYLETTDEAVDLAIEYITGKVVGQTSYADCLHMAL
                     ATINRADFLVSWNFKHIVNIQRIRGYNSINIKNGYRQLEIRSPREFEKYEDD"
     gene            complement(1783..3504)
                     /gene="ftsZ"
                     /locus_tag="VGA21_05725"
     CDS             complement(1783..3504)
                     /gene="ftsZ"
                     /locus_tag="VGA21_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019599351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEX9650038.1"
                     /translation="MESLFKFDIPKNKKSIIKVIGVGGGGSNAVNHMFNQGIRDVDFV
                     VCNTDAQALNSSPVPNKLQIGIGLTNGLGAGANPERGRSAALESKEDIREILDDNTKM
                     VFITAGMGGGTGTGAAPVLAKIAKDMDILTVGIVTVPFAFEGKKKMAQAELGIQMLKE
                     NCDTVLVIINDKLREIYGNLSISDAFAQADNILTTAAKGIAEIITVPGYVNVDFEDVR
                     TVMLQAGPAVMGSAKAEGDNRARKAAEQAISSPLLNNQDIHGAQKILLSITSGEQAEL
                     QMDELSEITGFIQEIAGPDAEVIFGHGIDSTLENKISVTVIATGFHTNANTQLLQHEM
                     RKVFDLETTRQIPLFQGKRIEPKSIPFEVEMNENIEKEYVPEEKEEVKEEIMEEDPAQ
                     LSMENQQEEEPEEKIRYTLEEEPGTGENHEQEEEVKEETEFDRGFFFDSKPLEVTDEQ
                     NENEESDILEEDDDDDLFEEEEIFKMADHAEEELDEEGILELKSKKEFLQKQARERVE
                     RLKGISGHDLDADTFKEKLDVPAYLRKKVVFEKVPHSSESNISRYNLNDDNQILGNNR
                     FLHDNVD"
     gene            complement(3535..4842)
                     /gene="ftsA"
                     /locus_tag="VGA21_05730"
     CDS             complement(3535..4842)
                     /gene="ftsA"
                     /locus_tag="VGA21_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009579616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEX9650039.1"
                     /translation="MEHEKIVVGLDIGTTKICAIVGRKNEYGKLEVLGLGKAVSDGVI
                     RGIVTNIDKTIQAIEKAVKEAEDQAGIDIRVVNVGIAGQHIRSTIHHGSITRGSMDDE
                     ITVEDVNKLTNDMYRIVIPPGSEIIHVMPQDYIVDYEEGIKDPVGMSGVKLEADFHVI
                     TAQTNAINNINKCVRRAGLEIEHLILEPLSSSLAVLSEEEKEAGVCLVDIGGGTTDIA
                     VFHDNIIRHTAVIPFGGNIMTADIKQGCMVMNHQAELLKTRFGKAIAEEASPNEIVSI
                     PGLRNRPPKEISVKNLSHIIEARMQEIIELIHTEIIVSGYENKLAGGIVITGGGALLS
                     CLKQLVEYMSGLDARVGYPNEHLGKSKVEAIKSPMYSTGVGLVLSGFRAIDERENRYN
                     EARLNSKPGMGRIRKEAGGDFFRKILDKTKGLLMDDIDGKNYY"
     gene            complement(4873..5637)
                     /locus_tag="VGA21_05735"
     CDS             complement(4873..5637)
                     /locus_tag="VGA21_05735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HEX9650040.1"
                     /translation="MNWRSKSLWLPIQIVCMAALVIIAISFVNRKQEETMYKDLLVSI
                     EDRYENFFVDEEDVIAMITDNGEKVFEGKFITELDIKLLEDKVKNESFIKDAEVYRDL
                     KGNVLVRATQRRPIARIISESGNHRYIAADGTVLPVSRKYTAKVVLIRGLETLKPGDN
                     ILNMEETLNLMDVLEFIYKDEFWKAQITEIEIQRNGDLILYTQVSRQYVEFGKPEIVE
                     NKFARLKIFYKDILPRKGWNVYERVNLKFKDQIICE"
     gene            complement(5621..7021)
                     /gene="murC"
                     /locus_tag="VGA21_05740"
     CDS             complement(5621..7021)
                     /gene="murC"
                     /locus_tag="VGA21_05740"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012929234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEX9650041.1"
                     /translation="MNLNKTDNIYFIGIGGIGMSALARWFILQGKNVAGYDRTQTALT
                     AQLESEGMLIHYEDDVNLIPQSFRDANNTLLVFTPAIPKDHKEYNYFNEKGFEIRKRS
                     EVLGQITKNRFTIAVAGTHGKTTTSSMIAHILKQNGHDMVAFLGGVSTNYENNFIYHN
                     PESKEAVVVAEADEFDRSFLQLKPDIAVITSADADHLDIYGDKLTMEESFKEFAGKIA
                     KAGTLFINNRIASKFQVPEGVDKKTYSRVDGDLKSEGLRIKGGNFIFDYQNGMGTIQD
                     LTLSVPGFHNVENLIAAVGVCLKLGLSKEEISSAVKTYTGVKRRFEYILKSENLVYVD
                     DYAHHPVEIEAFLKSMRSLYPGKKITVAFQPHLFSRTRDFTVEFAESLSLADKVILLD
                     IYPARELPLTGVTSEIIFDRITAKEKILCKHGELIGKLTAEKDLEVLATVGAGDIDQL
                     VEPIKNMLTKKYELEK"
     gene            complement(7018..8127)
                     /gene="murG"
                     /locus_tag="VGA21_05745"
     CDS             complement(7018..8127)
                     /gene="murG"
                     /locus_tag="VGA21_05745"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007652466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HEX9650042.1"
                     /translation="MNEVKSYRFIISGGGTGGHVFPALAVAHELRLQFGNPEILFVGA
                     KGKMEMTKVPENGYKIIGLNIRGLQRSLSFSNLLFPFRLISSLFKARSIIKEFRPDVA
                     IGFGGYASAPVLWVAAQMGVPTIIQEQNSYAGIANKILGKKAFKICVAYEEMDKYFSK
                     DKLVLTGNPVRKEIMNLENKKSEAISYFELDPGRKTLLVLGGSLGARSINESIIGNLQ
                     TLAESGIQMIWQTGQIYYEEMISRSVRINFPEIRIHKFLDRIDLAYSAADVVIARAGA
                     LTIAELCVAGKASILVPSPNVAEDHQTRNANYLAARDAALMIPDKEAVGKLVRQALEL
                     LNNTDKQEQLKTNSSAMAKTDAAREIVEQLKIVLK"
     gene            complement(8127..9284)
                     /locus_tag="VGA21_05750"
     CDS             complement(8127..9284)
                     /locus_tag="VGA21_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009579620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEX9650043.1"
                     /translation="MIKKWVDKNLQGDPVIWIIVFALSLISILVVYSATGTLAFRRME
                     GNTEYYLFKHTFLVILSFFAMWVAHKIDYRYYSKLSKIALWLSIPLLIYAWKAGVNVN
                     EASRWITIPIINQVFQPSDLAKLALIAALASMLSRRQQNIEDFKESLFPILLWCGVIC
                     GLIGMANLSTAILLFATCVLLMFIGRVPVKYLAMLMVVGLLAGSIVISVGQRGETGIN
                     RIKSFLDRKEIPFQAEQSFIAISTGGITGKGPGQSDRRNFLPHPYSDFIFAIIVEEYG
                     MIGALIVISLYLALLYRGMVTVANSQRAYGGLLSAGLSFALVIQAMVNMGVAVGLGPI
                     TGLPLPLVSMGGTSLLFTGISLGIILSVSRGEVDEEWNIQTGELKNIAKAA"
     gene            complement(9281..>10655)
                     /gene="murD"
                     /locus_tag="VGA21_05755"
     CDS             complement(9281..>10655)
                     /gene="murD"
                     /locus_tag="VGA21_05755"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015812944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEX9650044.1"
                     /translation="KIMAKKITILGAGESGVGAAILAIATGYEVFVSDSGSIKEEFKK
                     ELELRNIIYEEGLHSDIIFDSAEVIKSPGIPEDAEIITKIRSKNIPVISELEFASRFT
                     SAKIILITGTNGKTTTTLLTHHLLKSAGIDAGLAGNVGFSLARMIADDPKEVYVVEVS
                     SFQLDQSYKLKADIAVLLNITPDHLDRYGNDLTNYINSKFRVIQNMKAGDPFICNYDD
                     DIIKTRIESRNILPEVIKVSLRNEVETGGYLEGDIITTRLKEKEYRFNTIELPLKGNH
                     NAVNMMASILCAMNFGIGHQSILEGIKSFKNAPHRMEHVTAIKGIDFVNDSKATNVDS
                     AYYALGSYPAIVWIAGGKDKGNNYDQLREFAREKVKALVCLGKDNSKLLNYFRGVVPS
                     ISDTHTIKDAVRKAFSLAQKGNTVLLSPACASFDLFRNYEDRGDQFRNEVLELKKELE
                     KENNRVL"
BASE COUNT         2955 a         2446 c         1960 g         3294 t
ORIGIN      
        1 tttgacagag tcattttcat ggataaagta gaaaatgggc ttcgccaatc agaaaaaggt
       61 caaaccaaat caacctcaga ggcaacagct aagctgaaga aatggttgaa gtaagatgga
      121 ccgatcaagc tatccaggac aattgtttac aagatcttat ctaaaaagca aatcaacatt
      181 cttgctgttc ataatagcag catgattttg cgtaaggaag tcctcaaacg taagtaacgc
      241 gccctaacac agtgcaaatt tcaccctccg catctgataa ttttataaat ttgcttaagg
      301 tgctccgggc gaaaaatttg caccgggacc gttatcgctt gtgtaaaata aaatcaaccg
      361 gagactctaa ctaataattt taacaaaatg gtaaaaacac tactaatctc atttgcactt
      421 gcgacatcat tcggacttgg tttcacattc aacaatttaa taattaacca atcaaacaac
      481 gatattaaaa taaaaaggtt gagcaactca aaaaagaagg cgtaaccctt actgacaaaa
      541 tagaaacggt tgaatacgga gagttcgttc acatactgga tgttgaagga aaaaaagtgg
      601 aactatggga accaaacgac attgaatttg aaaaacttgg ttcccagttg ggtagcaaaa
      661 cgacaaaatg aatcaaaatt atttttccct caaactgttc gtagttagta accgtgaact
      721 caataaaaat taatcccctg atttatttga ttagagtttg ccgggaacat gcattcaagt
      781 ttctttgccg cctagttatg caaaaggcta acagactgat atcaaaacaa caaaacgacc
      841 gactgacttg aaaaaatcgt aactttgatt tgtgaaacaa agaatataca tagacacatc
      901 tgttttcggg ggctactttg acgaagaatt ttctgaacat acaattccac tttttgacag
      961 aattaaaaat ggagagttaa tattactcta ctcgacagta acacaagacg aactagaaaa
     1021 cgctcctgaa aaagtaaaag aacttgtaaa aggtcttaaa gccgacttta ccgaatactt
     1081 agagacaact gacgaagcgg ttgacctggc aattgaatac ataactggaa aagttgttgg
     1141 acaaacaagt tatgcagact gcttacacat ggcattagcg acgattaacc gggcagactt
     1201 tttagtaagt tggaacttca aacacattgt gaacattcag agaataaggg gttacaactc
     1261 gataaatatt aaaaacggtt acagacaatt agaaattcgt tcaccaagag aatttgaaaa
     1321 atatgaagac gactgaaaaa acatttgacg ctgtaaagta tatgcgtcaa caaagagaca
     1381 agttaagtga gaaattatct aaaatgacta aagaagaaat tttagagtat ttcaaaaaac
     1441 gtaagacaca gacgacaata aagccctgtg cataacagca cctacccaaa agtgtcggtt
     1501 cattggttaa tcaagctttg tgcttctatc aaattttttg cttgactgac atgaagcggt
     1561 tctaaatcgc catcttcggt agctgcgaac cgtcgggacg tgcggtaatt accctcggtt
     1621 tccggtattg ctgtcgaaca gcaatttata aacagcggca gaaaccggat gacaatctcc
     1681 agaattattt aatatgtcag accaaaaaag taaaacccgg cgttaaggca gcaaccgggt
     1741 tttcaccaac ttgaactaac aggctatctt gattatcaaa tatcagtcta cattatcatg
     1801 aagaaatctg ttattgccca ggatctgatt atcatcattc agattatacc tggatatatt
     1861 ggattcggaa ctgtgtggga ctttttcaaa taccactttc tttctcaagt aagcagggac
     1921 atcaagcttt tccttgaaag tatctgcatc caaatcatga cctgagattc ccttgagtct
     1981 ttccacgcgc tcccgggctt gtttctgaag gaactctttt ttggatttta attcaaggat
     2041 tccttcttca tccaactcct cttcggcatg gtcggccatt ttgaaaatct cctcttcttc
     2101 aaacagatca tcatcatcat cttcttcaag tatatcagac tcttcatttt cattttgttc
     2161 gtcggtaacc tcaagaggtt ttgaatcaaa gaagaatccc cggtcaaatt cggtttcttc
     2221 tttaacttct tcctcctgct catgattctc accggttcca ggctcctctt ccaatgtata
     2281 ccggattttt tcttccggct cttcttcttg ctgattttcc attgaaagtt gggccggatc
     2341 ttcttccata atttcctctt tgacttcctc cttttcttca ggaacgtatt ccttttctat
     2401 gttttcattc atttcaactt caaaaggaat tgattttggt tcaattcttt tcccttgaaa
     2461 aagcggaatt tgccgggtag tttccaggtc gaacactttc ctcatttcgt gttgcaagag
     2521 ttgagtgtta gcatttgtgt ggaaaccggt agcaatcacc gtcacactga ttttattttc
     2581 cagcgtcgaa tctattccgt gaccgaagat aacttcggca tcagggccgg caatttcctg
     2641 aataaacccg gtgatttcac tgagttcatc catttgcaat tcggcctgtt caccagaagt
     2701 aatcgatagc agaatttttt gcgcgccatg gatatcctgg ttgtttaata atggtgatga
     2761 aattgcttgt tcggcagcct ttcttgccct gttatctcct tcggcttttg ccgatcccat
     2821 tacggcgggg ccggcttgca gcataaccgt tcttacatct tcgaaatcca catttacata
     2881 accgggaact gtgatgatct ccgcgattcc tttagctgca gtggtcaata tattatctgc
     2941 ttgcgcaaag gcatcgctga tggataaatt gccatagatt tctctcagct tgtcattaat
     3001 gatcaccaga acggtatcac aattttcctt gagcatttga atccccagtt cagcctgggc
     3061 cattttcttt ttaccttcaa aagcaaaagg aacggtaaca atacccacgg tgaggatatc
     3121 catatccttg gcgatctttg ccaataccgg agcagctccg gtgccggtac cgccacccat
     3181 cccggcggtg atgaaaacca ttttagtatt gtcatccagt atttcccgga tgtcttcttt
     3241 gctttccaaa gccgcgctac gacccctttc gggatttgct ccagctccca aaccatttgt
     3301 aaggccaatg ccaatttgta atttgtttgg taccggagaa ctattcaatg cttgagcatc
     3361 agtattgcat accacaaaat caacatccct gatcccctga ttaaacatgt ggttaacggc
     3421 gttactgcct ccacccccaa caccaataac cttgattatt gatttcttgt tcttggggat
     3481 atcgaactta aataaacttt ccatttcgat ttagatttag tttaacgtag ttgattaata
     3541 ataattttta ccatcaatgt catccatcag gagacctttg gttttatcca gaattttcct
     3601 gaagaaatca ccgccggctt cttttctgat tctgcccatt ccgggcttac tgtttagccg
     3661 tgcctcgtta taacgatttt ctctttcgtc aatcgcacgg aaacctgaca gaacaaggcc
     3721 aacgccagtt gaatacatgg ggcttttgat cgcctccact ttgcttttgc ccaggtgttc
     3781 attgggataa ccaactcggg catccagtcc gctcatatat tccaccagtt gtttgaggca
     3841 gcttaacaaa gcaccgccgc cggttataac tattccaccg gcgagtttat tctcataccc
     3901 tgaaacaatt atttcagtat gaatcaattc aatgatctcc tgcatcctgg cctcaataat
     3961 gtgagaaaga ttcttgacag agatttcttt tggaggtcgg tttctcaatc cggggatgct
     4021 tacaatttca ttcgggcttg cttcttcggc aatcgctttt ccaaagcggg tttttaaaag
     4081 ctctgcctgg tgattcatta ccatgcagcc ctgctttata tccgcggtca tgatatttcc
     4141 accgaatgga atcaccgcgg tgtgacggat aatattatca tgaaagacgg cgatatcggt
     4201 agttcctccg ccgatatcaa ccaggcacac accggcttct ttctcttcct cgctaagtac
     4261 agcgagggat gatgaaagtg gttccaggat caggtgctct atttcaaggc cggctcttcg
     4321 cacacatttg ttaatgttat tgattgcatt tgtctgagcc gtgataacat ggaaatccgc
     4381 ttcaagtttt acacctgaca ttcccaccgg atctttaatt ccttcctcat aatccacaat
     4441 gtaatcttgt ggcattacgt ggatgatttc acttcccggt ggaataacaa ttctgtacat
     4501 atcattggtc agcttattca catcttccac agtaatttca tcatccatag accctcgggt
     4561 aatactacca tggtggatgg tgctcctgat gtgctgaccg gcgataccta cattaacaac
     4621 ccgaatatca atacctgcct gatcttcggc ctctttgacg gctttttcaa ttgcctggat
     4681 ggttttgtcg atattggtta caattccgcg gataactccg tcggaaactg cctttcccaa
     4741 accgagcact tcgagtttgc catattcatt cttacggcca actatcgcgc agatcttggt
     4801 tgtcccaata tcaagtccga caacgatttt ttcatgttcc ataattattt acagttagtt
     4861 ggtgattcaa ttttattcac agattatttg gtctttaaat ttcaaattca ctcgttcgta
     4921 cacattccaa ccttttcgtg gaagtatatc cttgtaaaag attttgagcc tggcaaactt
     4981 attctcaaca atttccggtt taccgaactc tacatactgc ctggagacct gggtgtaaag
     5041 aataagatct ccattcctct gaatttctat ttcagtaatt tgtgccttcc agaattcatc
     5101 cttgtaaata aattccagca catccattag attcagggtt tcttccatat taagaatatt
     5161 atctcccggc tttaatgttt caagtccacg tattaatacg accttcgccg tatatttcct
     5221 gctaaccggc aggacggtac catcagcagc aatataccgg tgattgccgc tttcggagat
     5281 tatccttgct atgggtcttc gctgggtcgc gcgcactaaa acattccctt tcaggtcccg
     5341 gtatacctcc gcatctttaa taaaactttc atttttcact ttatcctcaa ggagttttat
     5401 atccagttcc gtgatgaatt ttccttcaaa aactttttcc ccgttatcag tgatcatagc
     5461 tataacatcc tcttcatcca caaagaaatt ctcataccgg tcttcaattg aaaccagcaa
     5521 atctttatac atagtttcct cctgtttcct gttaacgaaa ctgattgcga ttatcaccag
     5581 ggctgccatg catacaatct ggatgggcag ccaaagactt ttacttctcc agttcatatt
     5641 tttttgttaa catattttta attggttcaa ctaactgatc aatatctcca gcacccactg
     5701 ttgccagcac ttccaaatct ttttcagcag ttaactttcc gatcaattca ccgtgtttac
     5761 aaagtatttt ctctttggct gtaatccggt cgaaaatgat ttcagaagta acgcccgtta
     5821 acggcagttc gcgcgccgga tagatgtcca acaggatcac cttatcagcc agcgagagac
     5881 tttccgcaaa ctcaacagtg aaatcgcggg tgcggctaaa caggtgaggt tgaaacgcta
     5941 cggtaatttt cttgccggga tacaggcttc tcattgattt cagaaatgct tctatttcaa
     6001 ccgggtgatg ggcataatcg tccacataaa cgagattttc tgatttcagg atgtattcaa
     6061 atcgtcgttt tactccggta taagttttaa cggctgaaga aatttcttct ttggataatc
     6121 caagtttaag gcaaacacct accgcggcaa tgaggttttc cacgttatga aatccgggaa
     6181 ctgaaagagt aagatcctga atggtgccca taccgttctg gtaatcaaaa atgaaattac
     6241 cgcctttaat tctcaagcct tccgatttaa gatctccatc tacccgggaa tatgtcttct
     6301 tatcaacacc ttccgggact tgaaattttg aagcaatcct attgttgatg aacagagtcc
     6361 ctgccttcgc gattttcccg gcaaattctt tgaatgattc ttccatggtc aatttatcac
     6421 catagatatc cagatgatcc gcgtcggctg aggtaatcac ggcgatgtca ggttttagtt
     6481 gaaggaaaga gcgatcaaat tcgtctgctt ccgctacaac cactgcttct ttgctttccg
     6541 ggttatggta aatgaagttg ttttcataat tagttgacac accgcccagg aatgcgacca
     6601 tatcatgtcc attttgtttc agaatgtggg caatcatgct ggaggtagtg gttttaccat
     6661 gagttccggc taccgctatg gtgaatctat ttttagtgat ttgtcccagt acttcagacc
     6721 gttttctgat ttcaaatccc ttttcattaa agtaattata ttctttgtga tcttttggta
     6781 tcgcgggtgt aaatactagt agtgtattgt ttgcatcccg gaatgactgc ggaatcagat
     6841 tgacgtcatc ttcgtaatga atcagcatcc cttctgattc cagctgagcg gttaaagcag
     6901 tttgggttcg gtcatagccg gcaacatttt taccctgcag aataaaccac ctggcaagag
     6961 cactcatacc gatgcccccg atgccaatga agtaaatatt atctgttttg ttcagattca
     7021 tttcagtacg atttttagct gctcaactat ctccctcgcg gcatcagttt tagccattgc
     7081 ggaagagtta gtttttaact gctcttgttt atctgtatta ttcaaaagtt caagggcttg
     7141 ccgcactaat ttcccgaccg cctctttatc cgggatcatt aaagctgcat ccctggccgc
     7201 cagataattt gcattccgcg tttggtggtc ctctgccaca ttgggtgaag gaaccagaat
     7261 gctggctttt cctgccacac ataattcggc gatggtaagg gcacccgccc gggcgattac
     7321 tacatcagct gctgaatagg cgaggtcaat gcgatccagg aatttatgga tccgaatctc
     7381 cgggaaattt attctcactg accgcgaaat catttcctcg taataaatct gtcccgtttg
     7441 ccaaatcatt tgaattccgg attcagcaag cgtctgcaaa ttcccgatta tgctttcatt
     7501 gatagacctt gctcctaaac ttcctcccaa caccagcaat gttttcctgc ctggatccag
     7561 ttcgaaataa gagattgcct cacttttctt attttccaaa ttcattattt cctttctgac
     7621 agggttgcct gtgagcacaa gcttatcttt agaaaagtat ttatccattt cttcataagc
     7681 cacacatatt ttgaatgctt tctttcccag tatcttgttg gcaatccccg cgtaagagtt
     7741 ctgctcctgg attatcgttg gaacgcccat ctgtgcagcc acccatagaa caggagcgct
     7801 ggcgtagccg ccaaatccga tggccacatc aggtctaaat tctttaatta tcgaccgcgc
     7861 cttgaataaa ctgctaatga gcctgaatgg aaacagcaaa tttgaaaagg aaagacttcg
     7921 ctgcaatccg cggatgttga gtccaataat tttgtaccca ttttccggaa cttttgtcat
     7981 ttccattttt cctttggctc ccacaaacag aatttcagga tttccgaatt gaagtcgtaa
     8041 ttcgtgagca actgccaatg caggaaatac atgaccaccg gttcctccac cactgataat
     8101 gaatcgatat gatttcactt cattcatcaa gccgctttcg caatattttt aagttcacca
     8161 gtttgaatat tccattcctc atcaacctca cccctgctta cactaagaat tatgcccaga
     8221 gagataccag taaataaaag ggacgttccg cccatactaa ccaaaggcag cggcagtccg
     8281 gttatcggac cgagaccaac tgccacaccc atattcacca tggcctggat tacgagcgcg
     8341 aaactcaatc ctgccgacaa aagaccgccg taagcacgct gactatttgc tacggttacc
     8401 ataccacggt ataacaaggc caggtacaag ctgattacta ttaatgcccc tatcatccca
     8461 tactcttcta ctatgattgc gaaaatgaaa tctgaatacg ggtgggggag aaaattcctt
     8521 ctatcgcttt ggcccggccc tttccctgtt atgcccccgg ttgatatcgc tataaaagat
     8581 tgttcagcct ggaatgggat ttcttttctg tccagaaatg atttaatccg gttaattccc
     8641 gtttcaccac gttgtccaac tgaaataacg atactaccgg caagcaaacc cactaccatt
     8701 aacattgcca gatattttac cggaactctt ccaataaaca ttaacagtac gcaagttgca
     8761 aacagcagga ttgcagttga caaatttgcc atacctataa gtccgcaaat cacaccgcac
     8821 cataaaagga ttgggaacag agattcctta aagtcttcaa tattttgttg tctgcggcta
     8881 agcatacttg ctaatgcggc tatcaaagcc agtttagcta aatcactggg ttggaaaacc
     8941 tggttaataa ttggaatggt aatccatctt gaagcttcgt taacgttgac tccggccttc
     9001 catgcataga ttaataaagg gatagatagc cataacgcta tttttgaaag ttttgaataa
     9061 tacctgtagt caattttatg agctacccac atagcaaaaa aggaaagaat aacgaggaag
     9121 gtatgcttga ataaataata ttctgtgttg ccttccattc tgcgaaatgc caaagttcct
     9181 gttgcactat atacgaccag gattgaaatc aaagacaatg caaatactat aatccagatt
     9241 accggatcac cctgaagatt tttatctacc catttcttta tcatagcaca cggttatttt
     9301 ccttttccaa ttcttttttc aattccagta cttcatttct gaactgatca ccccggtctt
     9361 cgtaatttct aaacaagtcg aaacttgcgc aggcaggaga aagcagcacc gtatttcctt
     9421 tttgtgcaag ggaaaaagct ttcctgaccg catccttaat ggtatgagtg tcggaaattg
     9481 atggtacaac tccccggaaa taattcagga gtttgctgtt atctttgcct aaacacacaa
     9541 gcgcctttac cttctcacgg gcaaattccc gcagctgatc ataattgttg cctttatctt
     9601 taccacctgc gatccagaca atggcgggat aacttcccaa ggcataatag gcagaatcga
     9661 catttgttgc tttggaatca ttcacaaagt caattccctt tattgctgtc acgtgttcca
     9721 tcctatgcgg tgcattcttg aaggatttta ttccttcaag aatactttga tggcctattc
     9781 caaaattcat agcgcatagt atagaagcca tcatgttaac cgcattgtgg tttcctttta
     9841 ggggaagttc aatggtatta aacctgtatt ctttctcctt taacctggta gtaattatat
     9901 caccttccaa atagccacct gtttcaactt catttcttaa agaaaccttt attacttcgg
     9961 gcaaaatatt tcgcgattca attcttgttt tgattatatc atcatcataa ttacaaatga
    10021 aaggatcacc tgctttcatg ttttgaatta cccgaaactt ggaattgata taattggtta
    10081 gatcatttcc gtatctgtcc aggtgatctg gtgtgatgtt gagcaggacc gcgatatccg
    10141 ctttgagctt ataggactgg tccagttgga aactgctaac ctctactaca taaacttctt
    10201 ttggatcatc ggcaatcatt ctggccaggc taaatccaac gttaccggcc aggcctgcat
    10261 caattcccgc agatttcaat aaatggtgag ttaaaagcgt tgtcgtagtc ttaccgttag
    10321 taccggtaat cagaataatt tttgctgaag tgaacctaga agcaaattca agttctgaaa
    10381 taacaggtat gtttttcgat ctgatttttg taatgatttc tgcatcctcg ggtatccccg
    10441 gactttttat tacctctgct gaatcaaaaa ttatatcgga atgcagccct tcttcataaa
    10501 taatattcct cagttccaat tcttttttaa attcttcttt aattgatcct gaatcggaga
    10561 caaacacttc atatcccgtg gctatcgcca atattgcggc cccaacacca ctttctccag
    10621 cacccaatat ggttatcttc tttgccatta tctta
//
LOCUS       DASWHX010000081         3222 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_62428, whole genome shotgun sequence.
ACCESSION   DASWHX010000081 DASWHX010000000
VERSION     DASWHX010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 3222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3222
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62428"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1371)
                     /locus_tag="VGA21_05760"
     CDS             complement(<1..1371)
                     /locus_tag="VGA21_05760"
                     /inference="COORDINATES: protein motif:HMM:NF015851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX9650045.1"
                     /translation="MKYGFVIDNRKCIGCHACTVACKSEHDVPIGVNRTWVKQVEKGE
                     FPNSKRVFSVLRCNHCTDAPCVEICPVTALFIRADGIVDFNNDRCIGCKSCMQACPYD
                     ALYIDPDTHTAAKCNYCAHRIEIGLEPACVNVCPEHAIISGDMDNPATEISEILAREQ
                     TTVRKPEKGTDPNVFYIDGDFTSLNPAATERSDQYIWGSQDKGVGHYSRFMENVSASK
                     DKEAMKHELENSTLSPAEISFAGESRKKSVEILMERAKASRVYDTPDKGILWGWEVSA
                     YILTKAIASGVIIIPFIGMLLGIDGISDTIIYTSGIVSVVFLLLTGILLVKDLDQPKR
                     FLYVLLRPQWNSWLTRGGYAITLMGGFTTFWLLGKYFGIGFLSNIFLWLAAIMALIVA
                     VYTAFLFAQAKGRDFWQSPVLVLHMMIHSFMAGSAAFTILFAMVQPATELITFTHTVL
                     AGSIIIN"
     gene            1667..1981
                     /locus_tag="VGA21_05765"
     CDS             1667..1981
                     /locus_tag="VGA21_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019941098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEX9650046.1"
                     /translation="MNNIIGSLEEIVLLLVMAKDEEIYGVSVAKQYTIHTGKSISIPA
                     VHTVLRRLESKKMIQSRVGGASKERGGRSKRIYDITNLGRKTLAEIRDNRSKLWELSL
                     AK"
     gene            1991..>3222
                     /locus_tag="VGA21_05770"
     CDS             1991..>3222
                     /locus_tag="VGA21_05770"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9650047.1"
                     /translation="MIKKNQSPPKWADQILEWFCDAEYLDEIQGDLHESFYQRINSKG
                     AWNARAYFILDVMKSFNSYNIRFKKKSKLYSQNLMIGNYLKIATRIMGKNSFYSGLNI
                     MGLALGIACSLLIIIFIIQERSYDSFHRDSERIFRITEKFEEDGELIENSASLPWSVG
                     PTLWTNYPDVKVTRMYQTWQKIPLMSYLEDDAHFYEDKVFFVDSSFFEIFSFPLIIGD
                     PKTVLKEPQTLVITEKMAQKYFPGEDPIGKVIQFENSLDFTVRGVAKDVPVNSHFHFE
                     FLAPLLNIGEIFKASGNRWHWEGWYWNPVHTYVKLPKRLEAQQFNAELAEFVIKNFPP
                     NLAPGIELKLQPLRDIHLKSNLYQELESNSSERSILVALSIAGFILLIAAINFINLTT
                     ARSFQRAKEVALRKVMGSA"
BASE COUNT          988 a          647 c          663 g          924 t
ORIGIN      
        1 attaatgatt atggaacctg ccaaaactgt atgagtgaaa gtaattaatt cggtggcggg
       61 ctgaaccatt gcgaacaaaa tggtaaatgc cgcggaaccc gccataaatg aatgaatcat
      121 catatgcaaa accaacaccg ggctttgcca aaaatctctt cctttagcct gggcgaaaag
      181 gaaagctgtg taaacggcaa caatcagtgc cataatcgcg gccagccata agaagatgtt
      241 ggagaggaaa cctattccaa aatattttcc gagaagccaa aatgttgtaa acccacccat
      301 tagtgtaatg gcatatccgc cacgggtcag ccatgaattc cattggggcc tcaataagac
      361 ataaaggaat cgtttgggtt gatccagatc tttaacgagt agaattccgg ttaagagcag
      421 gaatacaaca cttacgatac cagatgtata tattatggtg tcgctaatac catcaatacc
      481 caaaagcatt ccaatgaaag gaattataat tactcctgaa gcgatcgctt ttgtaagaat
      541 ataagcagaa acctcccatc cccacaaaat gcctttatcg ggagtatcat agacacggct
      601 tgcctttgcg cgttccatta gaatttcaac gctttttttt ctgctttctc cggcaaatga
      661 aatttcagca gggctcaagg ttgaattttc caattcgtgc ttcatggctt ctttatcttt
      721 tgaagccgaa acattttcca taaaacggga atagtgccca acccctttat cctgtgatcc
      781 ccagatgtat tggtcgctgc gctccgttgc agccggatta agcgaagtaa agtctccatc
      841 gatataaaat acattcggat ccgtaccttt ttcaggcttc cttaccgtgg tctgctcccg
      901 ggcgagaatt tctgaaatct ctgtagcagg attgtccata tccccggaaa taatcgcgtg
      961 ttccggacaa acatttacac aagcaggttc cagaccgatt tcaattctgt gtgcacaata
     1021 attgcatttg gccgccgtat gagtatcggg atcaatatat aaagcatcgt aaggacaggc
     1081 ttgcatgcac gatttgcagc cgatgcaccg gtcattattg aagtcaacaa ttccatcagc
     1141 cctgataaat aacgcggtta caggacaaat ttccacgcat ggagcatcag tgcaatgatt
     1201 gcacctcaac actgagaaca ccctttttga atttggaaat tctcccttct ccacttgctt
     1261 tacccaggtc cggtttacac cgataggaac atcatgttca gatttgcaag ccaccgtgca
     1321 ggcgtggcat ccaatgcact tcctgttgtc tataacaaat ccgtatttca tttactggat
     1381 ttagcgattt tttctaaagc tataaaaaaa taaacaattg aatagtgtga atgattttga
     1441 atagtgtgaa tgatttatgc tttgaaaccc ttcagcacgt ggaaataatt gtcaatcatg
     1501 gaccgagtgg gaagatcttt tgcggagtgt tcttcccttc aaagatttaa cttttccgtt
     1561 taccaaagct tttggaagga gttgataacc aaaagtttgt ctgaattaaa actcattaac
     1621 ctgttttttc gtatttataa tacaattgtt aatcaaatag atagaaatga ataatatcat
     1681 aggttccctg gaggaaatag tcctcctttt ggtaatggca aaggacgagg agatttatgg
     1741 ggtctctgtg gccaaacaat ataccatcca cacaggaaag tcaatttcga ttcctgccgt
     1801 gcatacggtt ttacggcgtc ttgaatctaa gaaaatgatt caatcccggg ttgggggtgc
     1861 cagcaaagaa aggggcggca gaagcaaacg catttatgat attaccaatt taggtagaaa
     1921 gacgttagcg gaaatcagag acaaccgatc aaaattgtgg gaactctcgc tggcgaaata
     1981 gtttttcaaa atgattaaaa agaatcagag tcccccgaaa tgggctgacc aaattttaga
     2041 atggttttgt gatgccgaat accttgatga aatccaggga gatttgcatg agtcgtttta
     2101 tcagaggatt aattccaaag gagcctggaa tgccagggct tattttattc tcgacgttat
     2161 gaaatcattt aatagttata atatcaggtt caaaaagaaa tcaaaattgt acagccaaaa
     2221 tcttatgata ggaaattatc tgaaaattgc cacccgcata atgggtaaaa attcttttta
     2281 ttccggactg aacattatgg gtctggcgtt gggcatagcg tgctcacttc tcatcatcat
     2341 tttcattatc caggaaagaa gttacgactc ctttcaccgg gattcagaac gaattttcag
     2401 aattacggaa aaatttgaag aagacggaga attaattgaa aattcagctt cattgccatg
     2461 gtcagttggg ccaacattat ggactaatta tccggatgtg aaggtaacgc gtatgtatca
     2521 gacctggcaa aagattcctc ttatgtctta cctggaagat gacgcgcatt tttatgaaga
     2581 caaagtattt tttgtggata gcagtttctt cgaaatattt agctttccgc taataattgg
     2641 ggatccgaaa accgtactta aagaacctca aaccctggtt attactgaga aaatggctca
     2701 aaagtatttt cctggtgaag atcccattgg caaagtgatt caatttgaaa atagtcttga
     2761 tttcactgtg cggggagttg ccaaagacgt tcccgttaat tcacactttc attttgaatt
     2821 ccttgctccc ttattgaata taggagagat ctttaaagca agtggaaaca gatggcattg
     2881 ggaaggatgg tattggaacc cggttcacac ttacgtcaag ctccccaaaa gattggaagc
     2941 gcagcaattc aatgctgagt tggctgaatt tgttattaaa aattttccgc caaacctggc
     3001 cccgggaatt gaattgaaat tacaaccact aagggatatt catcttaagt ccaatctcta
     3061 tcaggagctg gaatctaata gcagtgaaag atcaatactt gtagctcttt ccattgccgg
     3121 gtttatatta ttgatcgcgg caatcaactt tattaactta acaaccgcca gatcgttcca
     3181 aagagcaaag gaagttgcct tacgaaaagt tatgggttct gc
//
LOCUS       DASWHX010000082         5663 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_1547433, whole genome shotgun sequence.
ACCESSION   DASWHX010000082 DASWHX010000000
VERSION     DASWHX010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 5663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5663
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1547433"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(350..1180)
                     /locus_tag="VGA21_05775"
     CDS             complement(350..1180)
                     /locus_tag="VGA21_05775"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HEX9650048.1"
                     /translation="MIKRIINRAIYHLGRFLCQYRGKWFVDKIADKTYLFLRHLNNVN
                     FQPSKNGEHRIIEIISKHKPKCIFDVGANIGEWSQCVSQMNPEATIHSFEIVSSTYQQ
                     LLTSIKDNTNIRANNFGLSDKNESITISMADDSSTATSCKIIGMKSHEEYYKQDYECE
                     VRKASDYINSSKIAKIDFVKIDVEGMDLKVIKGFENEIDKVRVIQFEYGIFNIASHDL
                     LTDFCYFLNTRGFIVGKIFPLVVQFFEYQFQFENFHGSNYIAVRKDDMKLIDDLKRFG
                     "
     gene            1475..1762
                     /locus_tag="VGA21_05780"
     CDS             1475..1762
                     /locus_tag="VGA21_05780"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX9650049.1"
                     /translation="MHKYITGIVQKREQKMLSIFYMPDHIHLLVGSKPSMAISDLTKD
                     IKAGSSNFINDKKWLKGKFNWQEGFGAFSYSRSQIDTVIKYILNQEEHHKK"
     gene            2421..2579
                     /locus_tag="VGA21_05785"
     CDS             2421..2579
                     /locus_tag="VGA21_05785"
                     /inference="COORDINATES: protein motif:HMM:NF017757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650050.1"
                     /translation="MRLLAFWDKTDNKKTLVIATHGFIKKVDKVPANEIERAVRIRER
                     YFDKKTKR"
     gene            2598..2909
                     /locus_tag="VGA21_05790"
     CDS             2598..2909
                     /locus_tag="VGA21_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007656039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX9650051.1"
                     /translation="MKTYTLAEMKEKYIGKVGTKKRDDYEYELRMDVLGKMIKAARQE
                     RNLTQEELGKLVGVQKAQISKLESSTNSATIDTVIKVFKALKAEINFNVKIENNFVTI
                     S"
     gene            complement(3029..4024)
                     /locus_tag="VGA21_05795"
     CDS             complement(3029..4024)
                     /locus_tag="VGA21_05795"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEX9650052.1"
                     /translation="MKVAVIGVTGLVGQVFLKVLEERDLPVEILIPVASEKSIGSSVT
                     FRGKEWEIIGIDKAIEEVPDVALFSAGGAVSLEYAPKFATKGTVVIDNSSAWRMHKDI
                     KLVVPEINGAQLNENDKIIANPNCSTIQMVVALNELHKKYQIKRIVVSTYQSVTGTGK
                     DAVTQLMGERKRENVPKIYPHVIDMNAIPHIDTFLENNYTKEEMKMVNETRKILGNEN
                     IRITATCVRLPVMGGHSESVNVEFHNEFEITDVMNILSETEGVIVQDNVSENIYPMPL
                     LALGKDDVFVGRVRRDESQPKTLNLWIVSDNLRKGAATNAVQIVEYLQHCELIPT"
     gene            4070..>5663
                     /locus_tag="VGA21_05800"
     CDS             4070..>5663
                     /locus_tag="VGA21_05800"
                     /inference="COORDINATES: protein motif:HMM:NF013125.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HEX9650053.1"
                     /translation="MKQLLLVVLCFAHVILSAQVSEDFSDNEFLNSPAWFGDVTNFEI
                     LSQELHLIAPAVEGISYLATTSESINNATWEFRVRLDFNPSSSNYAKFYLVSDSANLA
                     GPLHGYFVRIGGSSDDISLIKQNGLVETILIDGLDGSINTSAVDVSVKVTRDDQGNWE
                     LFSDIGTTGVYYSEGVVQENDFARSFYCGLMCKYTSTRSDKFYLDNITISGDPYIDLF
                     PPMLANYSILNENSLMLVFNEPLLPGTVLPENFVLNSQLPIQIVFDSDNSSKVNLTFG
                     EAFTNGFNHQLFVQGISDNFNNVIEPFQLDFLYFVEIPAGKGDVIINEIFPDPSPAIE
                     LPEAEFIELYNTSTNPFQLAGWQITDGSTLTTLGSFLLLPDSMLIICPTAYVSQYAGF
                     GYVLGASPWPSQNNTGDFLIIRDAAGVVIDSVYYSNSWYGDENKDDGGYTLERINPVL
                     NCYNQSNWKASESESGGTPGSQNSVFNLEEDEIAPSVVQASFIAPNLLELILDEPVVE
                     QTIPHLTFTLTPEIETDSIIYLN"
BASE COUNT         1746 a         1069 c         1089 g         1759 t
ORIGIN      
        1 tccgtgcctg gttaattggt tggatttctc aatccaacac caaaaaactt ccgggcccgc
       61 aatccagtcg gcaacagtaa aataacaatg tggaatcctg cttcattgtg gatagtatat
      121 ttatcactca tgaagtatag ctttggttgg taaatttagt aatttgttgg gtggtagggc
      181 ggataaggat atcctgctat taagttggca cggatatgaa tatctgcgcc gactgtggga
      241 ggcaaagggt gggaaacttt ttattcattt ttaaggatga tatcaacagg taatctgaag
      301 gtaattattc taagtatcgg gtgggggctt gtctaatgaa ttgtctttgc tacccaaatc
      361 ttttgaggtc atctattaat ttcatgtcat cctttctaac tgcgatataa ttggatccat
      421 gaaaattttc aaattgaaac tgatactcaa aaaattgaac tacaaggggg aaaatttttc
      481 ccacaataaa ccctcgtgta tttagaaagt agcaaaaatc cgtcagcaga tcatgtgaag
      541 caatgttaaa aattccatat tcaaactgga ttacccgcac tttatcaatt tcattttcga
      601 atccttttat tactttgaga tccattccct caacatcaat ttttacaaaa tcgattttag
      661 ctattttact actgttaata taatcgctgg cctttcttac ttcacactca taatcttgct
      721 tgtagtattc ttcatgggat ttcattccaa taatcttgca gcttgttgcc gtagaggagt
      781 catctgccat actaatggta atgctttcat tcttgtcaga gagtccaaag ttattggctc
      841 ttatgtttgt attgtctttt atggaagtga gcagttgctg atacgttgag gagacaattt
      901 caaaagagtg aatggttgct tcgggattca tttgggatac acattggctc cattctccga
      961 tattagctcc tacatcaaaa atacacttcg gtttatgctt tgaaataatc tcgataatcc
     1021 ggtgctcccc atttttagag ggctgaaagt ttacattatt aagatgtctc aggaacaaat
     1081 aggtcttatc tgcaatttta tccacgaacc attttccgcg atattggcat agaaatctgc
     1141 ccaaatgata tatagctcga ttgattattc tctttatcat tagcaattgt tcaaaacact
     1201 caaatatagt ggttgctaaa aaagccgctg ggtaattatg tcacattcaa ttgtaaaaat
     1261 tgaacagaaa gggattttta gccctaccct caagcgtgag ctcccgttaa gtcccaaagg
     1321 agcaacctgt ttgtagaaaa tgaaacaatg caataagtaa gctccggagg agcgacctga
     1381 aatatgccaa acacctacac gcaaatttat attcagattg tctttgcagt caaaggcagg
     1441 gcaaatcttt atttaaaaat cacaccgcga agaattgcat aaatacatta ccggcattgt
     1501 gcaaaagcgt gaacaaaaaa tgctttcaat attttatatg ccagaccaca tacatcttct
     1561 tgttggttcg aaaccatcaa tggcaatttc agatttgaca aaagatataa aagcaggttc
     1621 gtctaatttt attaatgata agaaatggct aaaaggtaaa tttaattggc aggaaggctt
     1681 tggtgcgttc tcttattcaa gaagccagat tgatacggta atcaaatata ttttgaatca
     1741 ggaagaacat cataaaaagt aaaccttcag agaggaataa ttgatttcct gaaaaaattt
     1801 gaaattgaat acgatgaaaa atatttgttt gaatggataa ataaaacagg tcgctcctac
     1861 ggagcttacg gttgttgtat tctgcgataa ctacaaacag aatgctccta cagagcaaag
     1921 attaaaaatg aataaatgaa gaagaaagac aaactaatat caacccgcag taggccggat
     1981 attcatatcc gtgcctggtt aattagttgg atttctcaat ccaacaccat aaaacttccg
     2041 ggcccgcaat ccagtcgaca acagtaaaag tacgtgtaat cctacttctt ggttttgtaa
     2101 atatttgatt ttaaagccat aacgcaaacg ttagtcagaa gcttttatga aagattctcc
     2161 ctaagaattg atggaaaata tagtttctat aattggaaac ttttattatc tttgcctcgt
     2221 tgaaccagga catggttaat gagatatttt gacacgagat ttttagaaga agcggacgag
     2281 ttcattgcgg ccttaaaact aaagaccgtt aagaaaatac tttacaacat tgacctcgca
     2341 gaacagacaa atgacccaag attttttaaa aattaaagaa agatatttgg gagtttagga
     2401 cgaagtacga aggacttcaa attcgacttt tagcattttg ggataagact gacaacaaga
     2461 aaactttggt aatcgcgacc catggattca ttaaaaaagt tgataaagtt cctgcaaacg
     2521 aaatagaaag agcagtaaga attagagaac gatattttga caaaaaaaca aaaagataaa
     2581 tcattatggc aaaaagaatg aaaacataca cacttgccga gatgaaagag aagtacatcg
     2641 gcaaagttgg gacaaagaaa agagatgact acgaatatga acttcgtatg gacgttttgg
     2701 gaaagatgat taaagcagca agacaagagc gtaacttgac tcaagaagag ttgggaaaac
     2761 ttgtgggtgt tcaaaaagcg cagatatcca aacttgagag cagtacaaac agcgcgacaa
     2821 ttgacacagt aattaaagtt tttaaagcat taaaagctga gataaacttt aacgtaaaaa
     2881 ttgagaacaa ctttgtaaca attagttgaa agaaaaccga cgcccaacaa gaaggcttaa
     2941 acgaagccgg cgcacagtgt agcatgctac gaagctgaga gctgtcatgg atggggttca
     3001 atttaagcaa tccaaaaaga tcgggttttt atgtgggaat cagttcgcaa tgttgcaaat
     3061 attcgacaat ttgaactgca tttgtagcgg cacctttacg aagattgtca gaaactatcc
     3121 acaggttcag cgttttcggt tgtgattcat cccttctgac tcttcccaca aacacatcat
     3181 ctttacctag agcaagcagt ggcatggggt aaatattttc tgatacatta tcctgaacaa
     3241 ttaccccttc agtttctgaa agaatgttca ttacatctgt aatttcaaat tcgttatgga
     3301 attcaacatt aacagattcg gaatggccgc ccatcaccgg aagtcgtaca catgtcgcag
     3361 tgattcgaat attttcattc cctaaaattt ttcgggtttc attcaccatt ttcatttcct
     3421 ctttcgtgta attgttttcg agaaatgtat caatgtgcgg tatcgcgttc atgtcaatca
     3481 cgtgtggata aattttcggc acattttcgc gttttctctc tcccataagt tgagtcacgg
     3541 catctttacc tgttcccgta acggactgat acgtgctcac tacaattctt ttgatttgat
     3601 acttcttgtg aagctcattc aatgcgacaa ccatttggat tgtagagcaa tttggattgg
     3661 cgattatttt atcattttca ttgagctgcg ctccattaat ttcaggcaca accagtttta
     3721 tatccttgtg catcctccag gctgaggagt tatcaattac aaccgtacct tttgtcgcaa
     3781 attttggagc atattcaagg gatactgctc ctcccgcact aaacaatgct acatcgggta
     3841 cttcttcgat ggctttatca atcccgatta tttcccattc cttcccccgg aaggttactg
     3901 atgaaccgat ggatttctcc gacgctaccg ggattagaat ttccaccggg agatctctct
     3961 cctccaatac tttaagaaaa acctgaccca ctaaacccgt aactcctatt acggctactt
     4021 tcatttatga ttattttgtg tttactttat acttttcaaa taaaaggata tgaaacaact
     4081 tctacttgtt gtactgtgtt ttgcgcatgt tatcctatca gcgcaagtta gcgaggattt
     4141 ttcagataat gagtttttga attccccggc ctggtttgga gatgtaacta atttcgagat
     4201 attgtctcag gaattgcatt tgattgcacc tgccgtggaa ggtatttctt acctcgccac
     4261 aacctcagaa agcataaaca atgccacctg ggaattcaga gttcgtttgg atttcaatcc
     4321 gtcttccagt aactacgcga aattttacct ggtttctgac tccgctaatc ttgccgggcc
     4381 gctgcacggt tactttgtaa ggataggcgg cagttccgat gatattagtc ttataaaaca
     4441 aaatggactt gttgaaacaa ttttgattga tggtctcgat ggcagtatta atacttctgc
     4501 ggtggatgtc tcagtaaaag ttaccaggga tgaccagggc aattgggaat tgttctctga
     4561 tattggtaca accggggttt attactccga gggtgtggtt caggaaaatg actttgcacg
     4621 gtcattttat tgtgggttaa tgtgtaaata cacttcaacg cgatcagata aattttacct
     4681 ggataatatt actatctccg gagatcccta tatagattta ttcccaccaa tgcttgctaa
     4741 ctattctatt ctcaatgaga attcactgat gctggttttc aatgaaccgc tacttcccgg
     4801 tactgttttg cccgaaaatt ttgttttgaa ttcgcagctt ccgatacaaa tcgtgtttga
     4861 cagtgataat tccagcaagg taaatctaac ctttggtgaa gcatttacga atggatttaa
     4921 tcatcaacta tttgtgcagg ggatttcaga taattttaat aatgttattg aaccttttca
     4981 gctagatttt ttatacttcg tagaaatacc agcaggaaag ggtgatgtta taattaatga
     5041 aatctttcct gatccttctc ctgcaataga gcttcctgaa gctgaattca ttgagctcta
     5101 taatacatca acaaaccctt ttcaattagc cggttggcaa attaccgacg gctcaacgct
     5161 tacaactcta ggctcattct tacttctccc cgattcaatg ctaataattt gtccaaccgc
     5221 ttatgtatct cagtatgccg gtttcgggta tgttcttggc gcatctccct ggccaagcca
     5281 gaataatacc ggcgattttc tgattatcag agatgcggca ggtgttgtga tagattctgt
     5341 ttattattcc aattcctggt acggtgatga aaacaaagat gatggcggat atacgcttga
     5401 aagaataaat ccggttttga attgttataa ccagagcaat tggaaggcct ccgaaagtga
     5461 atcaggaggc acacccggta gtcaaaattc cgtattcaat cttgaggaag atgagattgc
     5521 accttccgtg gttcaggctt cattcattgc tcccaattta ttagagttga tacttgatga
     5581 gccggttgtt gagcaaacaa tacctcacct gacttttact ttaacgccgg aaattgaaac
     5641 tgatagtata atatatctga atg
//
LOCUS       DASWHX010000083        12637 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_3382315, whole genome shotgun sequence.
ACCESSION   DASWHX010000083 DASWHX010000000
VERSION     DASWHX010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 12637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12637
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3382315"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="VGA21_05805"
     CDS             <1..198
                     /locus_tag="VGA21_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015812089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HEX9650054.1"
                     /translation="FKKEVFTIWGNTIPEFGKYPYKTKFKILQNNRLDCRPCSAKGYD
                     KCPKGHLKCLNEIIFDFYFPE"
     gene            232..846
                     /locus_tag="VGA21_05810"
     CDS             232..846
                     /locus_tag="VGA21_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013407443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4254 domain-containing protein"
                     /protein_id="HEX9650055.1"
                     /translation="MKAKDCNRIFNESVVNYHKTDKIDAASQNPYSEGSIEELLYKKN
                     WIDTVQWHLEDLIRDPDISPAELVELKRRIDKSNQDRTDTVEKMDDWFLNFFGNIELK
                     QGAGMNSETPAWLLDRMSILTLKVYHMAEQVERKDVGEEHINKCKLKLEILIEQSSDM
                     ELCFDQLVKDIRNGDRYMKVYRQMKMYNDNTLNPVLYKKSAQNP"
     gene            843..1895
                     /locus_tag="VGA21_05815"
     CDS             843..1895
                     /locus_tag="VGA21_05815"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEX9650056.1"
                     /translation="MNSDPVKNILVVRFSSLGDLGLLYPALTSFLHQHSAYTIILVTK
                     EDFAPLFHGIERLIIYPVNTKKYKGFFGMYKLSELLNNSFNFKTVIDAHQVLRTFLLN
                     FFLRIKGKEVFRLKKSRKERNALTRKHNKIFQPLPHATEQYLNIFREAGFPTKLEHGP
                     YLPDAENSFAKSVFEELKISDDKKLIGIAPFAKWQTKVLPLHKMEHLLKLISESGKFN
                     VLLFGSLKEAPHLKLWEEKFSSTYYVGGKGDLSDEIHLMKKLSLLVSMDSANMHLGAL
                     AGIRLMTIWGSTHRYAGFGPVNSGEELPIEISEKELTCRPCSVFGDKPCWRGDHACMN
                     LIDIHKVFDEIVRITN"
     gene            1904..2398
                     /locus_tag="VGA21_05820"
     CDS             1904..2398
                     /locus_tag="VGA21_05820"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX9650057.1"
                     /translation="MPSLYLIRHAKSSWKQPELEDSKRPLNNRGERDAPEMARRLKLQ
                     SIIPDLIISSPALRARKTAEIISSEMGYDESEIKYDWELFHASTNTLLSILKKIPPRF
                     RIVFLFGHNPGLTYFANSLSNLKIDNIPTTGIVGIEFNKPWKDINFGTGKMLLYDYPK
                     KVKE"
     gene            complement(2388..3791)
                     /gene="hslU"
                     /locus_tag="VGA21_05825"
     CDS             complement(2388..3791)
                     /gene="hslU"
                     /locus_tag="VGA21_05825"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013452962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HEX9650058.1"
                     /translation="MDIENFLTPRQIVEELDKYIIGQDDAKRNVAIALRNRWRRMMAD
                     PEIQNEIIPNNILMIGATGVGKTEIARRLAKIADAPFTKVEASKFTEVGYVGRDVESM
                     VRDLVEQSVNLVKTRKKEEVKEKAEQIVEEIILDALIPPVKTNPQVSSQVYSTEKSET
                     GEQQLSEEELNERTRDRFRQKIKNRELEQRKIEILLRQPVNPGIGMIGGGVMDESSMI
                     NLQEMLNGMMPKRPKKRKVTIEEARKILLEEEVAKLIDMDEVKEEAIRKAQNTGIIFI
                     DEIDKIASSTRTSGGPDVSREGVQRDLLPIVEGTTVNTKYGHIQTDHILFVAAGAFHV
                     SKPSDLIPELQGRFPIRVELKNLTKQDFIRILSEPKNALSRQYQALFKSEKVTLEFHG
                     KSLESIADFAFTINENVENIGARRLHTVMSHLLNEFLFDVPDKIGENAHVVITKEMVE
                     EKLSTLVKNRDLSQFIL"
     gene            complement(3803..6277)
                     /gene="lon"
                     /locus_tag="VGA21_05830"
     CDS             complement(3803..6277)
                     /gene="lon"
                     /locus_tag="VGA21_05830"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013452960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEX9650059.1"
                     /translation="MDRIHFFLNKHLLGNPESSNDGEYIQIVNESGDNSPKKEDIPDE
                     LSLLPVKNTVLFPGVVIPITVGRKKSIRLVKKAYESKRIIGVIAQSNSKAEEPALKDL
                     YKVGTVAKIIKMLVLPDGSITIIIQGSHRFEIKSIIQEEPYYTVSIKILEDKFLAGGQ
                     KETNALVQSIKDSASKILRLNPEIPQEAQIALDNIHDPGFLTHFLSSNLNVDVSDKQK
                     LLEAENGQKRATLLLQYMLKEVKLLELKQEIQKKVNTDIDQQQRDYYLRQQIKVLQDE
                     LGYDGPDRDLADLRDKGAKKLWPEYVARHFTKELEKIQRMNPAAAEYPVAMNYAEFLI
                     DLPWSEVTKDNFDLNRAKEILDADHFGLEKVKDRILEYLAVLKLKQDMKGPILCLYGP
                     PGVGKTSLGKSIAHALNRHYVRVSLGGLHDEAEIRGHRKTYVGAMPGKIIQNIKKAKT
                     SNPVFILDEIDKVNSDFRGDPASALLEVLDPEQNHSFQDNYLELEYDLSKVLFVATAN
                     SLETIHPALRDRMEVIEINGYTLEEKIEIAKRHLIPKQRNEHGLKTKQVNFHKNALVK
                     LIDGYTRESGVRNLERQIGTVIRKIARAVAMKKKYNTTITDKRIIELIGPEIFDKEFY
                     QENKIPGVATGLAWTPTGGEILFIESSLSKGKGKLTLSGHLGEVMKESAMAALSFLKS
                     YHESLGIDYRAFDKYDLHIHVPAGAVPKDGPSAGITILCSLASVFTQRTIRPKVAMTG
                     EITLRGTVLPVGGIKEKILAAKRAGIKEIILCHRNKKDIEEIESSYIRDLKIHYVSTV
                     AEVLNKALQGKKVKGALEFELDLKAD"
     gene            6459..8429
                     /locus_tag="VGA21_05835"
     CDS             6459..8429
                     /locus_tag="VGA21_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009578793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX9650060.1"
                     /translation="MTSGKIITFLLLMFTLSYEGSFGQRSVEKRLFSPEFIKKDISTY
                     SQSDLFDFPNINLVPFYYHRSEMNRIQNLENRKNWKDLYPVLRAYVKNFGIENFYKDT
                     YWVWRLAKLTEEMGNHDEALLLYRLVLKHHREDIDITKVELHYDSLIANDKDYFVPID
                     YYYELVEFRKEVDTLRPPRGVLINMGKDINSETSDYAPSLNMADKMLIFTSKRKVINP
                     GMGEEPNEDLFFSINDDGYWRPAQSFPDLNTLYNEGSACMSKDGKTLYFSRCYSPESY
                     GNCDIFVAKMQFDGKWSDVANMGPNINSLAWDSHPSLNHTEDTLYFASDRIGGFGLAD
                     IYFTYKDANGRWMPAKNAGPIVNSRANEISPFFHHKDEILYFSSNGHAVNFGAFDIYK
                     TQRVRSSWSEPKNIGPLVNGSGNEFYFTIDSESRNLYYSHSTEKDINNLDLYSFPVPM
                     EAQPGATTTLEGSLNDGSDEPFKGIVSIIDMDNGIEVAPKFLRPDGTFEFNLINNNNY
                     LLIIRGEEFLKIEELFFLDGNTEFLQTTENIKSTIEFESIQFGNEESNINGSMFGDLN
                     KVGEFLLDNPEFTLKISGHTDSDGREEFNLELSQKRADAIKEYLVYFNSVEPERIISE
                     GYGSSKPLVKEVTDADKSVNRRVEFQLVKGGG"
     gene            complement(8432..9769)
                     /gene="rseP"
                     /locus_tag="VGA21_05840"
     CDS             complement(8432..9769)
                     /gene="rseP"
                     /locus_tag="VGA21_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009578790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEX9650061.1"
                     /translation="MEGLIMTAQILLSLSILVGLHELGHLVAAKAFGMRVEQYSIGFP
                     PKIYGVKYGETEYSIGAIPLGGYVKISGMIDESFDTNHLKEEPKDYEFRSKPAWQRLI
                     VMMGGITVNVFLGIFISIGIVWYYGDTFISKEDANKYGIVALEIGRELGFETGDKIVK
                     INGEDYQSFGDIQSPNVLLSNGGYYTVERDGKLVNIPIPIDFIEKLSDRKAAQQFIEI
                     RYPWRIASIPEDSDALTSGLRPGDKIIEMAGESTLYRDQFESIKDQHVGEDVPVVVER
                     AGDLFNLTVKINEEGTIGFYTEAIIDFSHKNYSFFESVPEGTGRAFMAVWVNIKALGK
                     IFRGEVSASKSISGPIGIAQIFGGTWDWARFWSITGLLSMVLAFMNFLPIPALDGGHV
                     MFLSYEIISGRKPSDKFLEGAQKIGVVFLLALMAFIIFNDIFKLEAIKGLFGG"
     gene            complement(9769..10929)
                     /locus_tag="VGA21_05845"
     CDS             complement(9769..10929)
                     /locus_tag="VGA21_05845"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009285318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HEX9650062.1"
                     /translation="MDSIKKNIAILGSTGSIGRQTLEVIGKHPDQFQVEVLTSNNNSE
                     LLVEQSKRFKPNAVVIVNDNEYEFVKNELTGLNIKVFSGVKSLASIVEMETIDMVLTA
                     LVGYSGLLPTVNAIKAGKEIALANKETLVVAGEWITSLAREAGVNIFPVDSEHSAIFQ
                     CIVGEFHNPIEKIILTASGGPFRGKSQEFLTSVTKEQALKHPNWDMGAKITIDSATLM
                     NKGLEVIEAKWLFGLDAGQVEVVIHPQSIIHSMVQFQDGSIKAQMGVPDMRLPILYAL
                     SYPNRLKSDFPRYSFENYPTLTFEKPDLKTFRNLELAYKSINFGGNMPCILNAANEIA
                     VENFLNDKIGFLRMSEVVETCMEKVPFIPKPGYEDFVNTDKETRIKAREIIK"
     gene            complement(10937..12436)
                     /locus_tag="VGA21_05850"
     CDS             complement(10937..12436)
                     /locus_tag="VGA21_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020530732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HEX9650063.1"
                     /translation="MGIRSILAKPFAAYVAKETIAWSSAPAESQKKIFENLISTGSQT
                     LFGKQHNFDSIKNYNDFKSQVPVRDYEDLKIYMEKIVKGERNVLWPGIPKYLSKTSGT
                     TSGVKYIPLTKESIPNHINGARNAILSYIHETGKSSFSDKKLIFLSGSPQLDFTGKIP
                     TGRLSGIVNHHVPSWLRGNQLPSIETNCIEDWEEKLERIIDETLAQPMSLISGIPPWV
                     QMYFDRIHDRTQKLIKDVFPDFSLFIYGGVSFEPYRKKLFESIGKEIDSIETYPASEG
                     FIAYQDSQNKNGLLLLLNSGIFFEFIPADEFFNEKPSRLMIDQIELNKNYAVVLSNNA
                     GLWSYSLGDTVKFISDSPFRLVVTGRIKHFISAFGEHVIGEEVEKAMEHACSSQPGTE
                     IIEFTVAPNVSPESGLPHHEWYIEFRSPPVDLSKFIYELNLRLQELNPYYDDLISGKI
                     LSNLKIISLRRDSFREYMKQIGKLGGQNKVPRLSNDRKIADELIKYAII"
BASE COUNT         3717 a         2606 c         2353 g         3961 t
ORIGIN      
        1 tttaaaaagg aggtatttac tatttgggga aatacaattc ctgaattcgg aaaatatccg
       61 tacaaaacta aattcaaaat attgcaaaat aaccggctcg attgcagacc ttgcagcgct
      121 aaaggatatg ataaatgtcc taaaggacac ctgaagtgtc tgaacgagat tatttttgac
      181 ttttattttc ctgaataaag aagttaattc ctgttgactt atttttgaag tatgaaagcg
      241 aaggattgca acagaatttt taatgaatca gtcgttaatt atcataagac ggataagata
      301 gacgcggcga gtcagaatcc ctattccgaa ggttcaattg aggaattgct ttacaaaaaa
      361 aattggattg atactgtgca gtggcacctt gaagatttaa tcagagatcc ggatatttct
      421 ccggccgaac ttgttgagct gaaaagaagg atagataaat caaatcagga tcgaacagat
      481 acagttgaaa aaatggatga ctggtttctt aatttctttg gtaatattga attaaaacaa
      541 ggagccggaa tgaattcaga aacaccggcg tggctcttgg acagaatgtc aattctaact
      601 ttaaaagtat atcacatggc tgagcaagtg gagcgaaaag atgtcggcga agagcacatt
      661 aataaatgta aattaaaact tgagatctta attgagcaat cttctgatat ggagctttgc
      721 ttcgatcagc ttgttaagga catcagaaat ggtgatcgat atatgaaggt gtacaggcaa
      781 atgaaaatgt ataacgataa taccctaaac cctgtactct ataaaaaatc agcacaaaat
      841 ccttgaattc agatccggtc aaaaatattc tggttgttag gttttcgtca ctgggggatt
      901 taggattact ttacccggca ctcacctcat ttcttcacca acattcggca tatacaatca
      961 ttcttgtcac taaagaagac tttgcgccgc tgtttcatgg aattgaaagg ctaatcattt
     1021 atccagtaaa cacaaaaaag tataaggggt tttttggaat gtataaatta tctgagcttc
     1081 taaataattc attcaatttc aaaactgtaa ttgacgcgca ccaggttctt agaacttttc
     1141 ttctcaattt ttttttacgc attaagggca aagaagtatt cagacttaaa aaatcacgca
     1201 aagaaagaaa tgcgttaaca agaaagcata ataagatctt ccaacctttg ccccacgcga
     1261 ccgagcagta tttgaatata ttcagagagg caggttttcc tacaaagctt gagcatggac
     1321 catatttacc ggatgcagaa aattcatttg caaagtctgt ttttgaagaa ttgaaaattt
     1381 ctgatgacaa aaaattaatt ggcattgcac catttgcaaa gtggcaaacc aaagttttac
     1441 ctctacataa gatggagcat ttattaaagc tgatttctga atcgggcaaa tttaatgtgc
     1501 ttttatttgg aagcctgaag gaagctcctc atcttaaact ttgggaagag aagttttcca
     1561 gcacctatta tgtgggtgga aagggcgatt tgtcagatga aattcatctc atgaaaaagc
     1621 tttcgctgct ggtaagcatg gattcagcaa acatgcatct gggtgccctg gccggaatcc
     1681 gcttaatgac catttgggga tcaacgcatc ggtacgcggg tttcggcccg gttaattccg
     1741 gagaagaatt acctattgaa atttcagaaa aggaacttac ttgcagacct tgttcagtat
     1801 ttggcgataa accctgctgg cgaggcgacc atgcatgcat gaatttgatt gacatccata
     1861 aggtttttga cgaaattgtc aggataacca attaacccaa atcgtgccgt cactgtacct
     1921 gattcgacat gcaaaatcaa gttggaagca accggagctt gaagattcga aaagaccact
     1981 caacaaccgc ggtgagcgtg acgcgcctga aatggcaaga cgattaaaat tacaaagtat
     2041 aatacctgat ctgataatat ccagcccggc cttaagagcc cgaaaaaccg ctgagataat
     2101 tagttcagaa atgggatatg atgaaagtga gatcaaatat gactgggaac tttttcatgc
     2161 cagtacaaat accctgctta gcattctaaa aaagatacct ccccgattta ggatagtttt
     2221 tctcttcgga cacaaccccg ggttaaccta tttcgctaat tctctgagca atcttaaaat
     2281 tgataatatc ccgaccaccg gcattgtggg cattgaattc aataaaccgt ggaaggatat
     2341 aaattttggt accgggaaaa tgcttttata cgattatcca aaaaaagtta aagaataaat
     2401 tggctcaaat ccctgttttt tacaagagta cttaactttt cctccaccat ttcttttgta
     2461 ataaccacgt gcgcgttttc tccgatttta tcaggaacat caaacaagaa ttcattgagt
     2521 aaatgactca tgaccgtgtg taaccttctt gccccaatat tttcaacatt ttcattaatc
     2581 gtaaatgcaa aatctgcaat cgactccagc gatttgccat gaaattccaa agtaaccttt
     2641 tcagatttga acaaagcctg atattgtctg gaaagcgcgt tcttgggctc gctcaaaatt
     2701 ctaataaaat cctgcttggt gagattttta agttctactc tgataggaaa tctgccctga
     2761 agttccggaa tcagatccga tggcttggaa acatggaatg cacccgcggc aacaaacaag
     2821 atgtgatcag tttggatgtg accatactta gtattaacag tcgtcccttc cacaatcgga
     2881 agcaaatctc gctgaacacc ttctctgctt acatcaggac ctccgctggt tcttgtactt
     2941 gaagctattt tgtcaatttc atcaataaag ataattccgg tattttgagc ttttctaatt
     3001 gcttcttctt taacctcgtc catatcaatg agttttgcaa cttcttcctc cagcaaaatc
     3061 tttctggctt cctcaatggt tacttttctt ttcttgggtc gctttggcat cattccattc
     3121 agcatttcct gcaggttaat catagatgat tcatccatca cacctccgcc gatcatccca
     3181 ataccaggat tcaccggttg ccttaacaat atctcaattt tgcgctgctc caattcccgg
     3241 ttttttattt tttggcggaa ccggtctctc gttcgctcat tcaactcctc ttctgacaac
     3301 tgctgctcac cggtctctga tttttcagtc gaataaacct gagatgatac ttgtgggttg
     3361 gtttttacag gtggaatcag agcatcaagt ataatttctt ccacaatttg ctccgctttc
     3421 tcctttactt cctctttctt ccttgtctta accaaattca ccgattgttc cacaaggtct
     3481 ctaaccatac tttcaacatc acggcccaca taccctactt cggtaaattt gctggcttct
     3541 acttttgtaa aaggagcatc tgcaatctta gcaagtctgc gggcaatttc agttttaccg
     3601 acaccggttg cgccaatcat cagaatatta tttggaatga tttcattctg gatttcagga
     3661 tccgccatca tccgtctcca ccggtttcgc aatgcaatag ctacatttcg ttttgcatca
     3721 tcctgtccaa taatatattt atccagctct tcaactatct gtctgggtgt taagaaattt
     3781 tcaatatcca taatttgatt tgctaatctg ctttaaggtc taattcaaat tcaagagcac
     3841 cttttacttt tttaccttga agagctttat ttaagacttc agcaacggtg ctaacataat
     3901 ggatcttcag gtcgcgaatg taagatgatt caatttcttc tatatctttt ttattccggt
     3961 ggcatagaat aatttccttt atgcctgccc gcttagctgc taaaatcttt tctttgatcc
     4021 caccaaccgg caatacagtt cctctaagag ttatttctcc ggtcattgct acttttggtc
     4081 ttatagttct ttgagtaaat acagaagcca aagagcaaag aatagtgata ccggctgatg
     4141 gaccatcttt cggtaccgct ccggctggaa cgtggatatg gagatcatat ttatcaaatg
     4201 cgcgatagtc aataccaaga ctttcatgat atgatttcag gaatgaaagg gcggccattg
     4261 ccgattcctt catcacttct cccaaatgac cggagagagt cagttttcct ttgcccttgc
     4321 tcaatgagga ctctatgaaa agtatctcac ctccggtggg tgtccatgcc aatcctgtag
     4381 ctactcccgg aattttgttt tcctggtaaa attccttgtc aaagatttca ggcccaatta
     4441 attctataat ccgtttatca gtgattgtag tattatattt tttcttcatt gccactgccc
     4501 ttgcaatctt cctgattacc gtgccgattt gcctctccag atttctcact ccagactccc
     4561 gcgtataacc atcaatgagc ttaactaatg catttttatg aaaattaacc tgtttggttt
     4621 tcaatccatg ttcattccgt tgtttaggaa tcaaatgacg tttcgcaatt tctattttct
     4681 cttccagggt gtagccatta atttcaatta cttccattct gtctctcaga gccgggtgaa
     4741 tggtttccag ggaattagct gtagccacaa acaaaacttt tgacaggtca tactcaagtt
     4801 cgaggtagtt atcctgaaaa gaatgatttt gttccggatc cagaacttca agtaaagcag
     4861 aggccggatc tccgcgaaaa tcagaattta ctttatcgat ttcgtcaagg atgaaaacgg
     4921 ggttagatgt ttttgccttt ttgatattct gaataatctt gcccggcatt gcccccacat
     4981 acgtctttct gtgtccgcga atttcagcct catcatgtaa gccccctaaa gaaacccgga
     5041 cataatgccg gttaagggca tgagcaattg atttacccag tgaagtctta cccacgccgg
     5101 gaggtccata taagcataga attggtcctt tcatgtcctg tttcaatttc agaactgcca
     5161 gatattcaag aatccggtct tttacttttt ccaatccaaa gtgatcggca tctaaaattt
     5221 ctttggccct gttaagatca aaattgtctt tggttacttc actccagggc aaatcaatca
     5281 ggaattcagc atagttcata gccaccggat attcagctgc cgccggattc attctttgaa
     5341 ttttttccaa ttctttagta aaatgcctgg ccacatattc tggccacagc tttttcgctc
     5401 ctttatctct caggtcagcc aaatctctgt ccgggccgtc atatccaagt tcatcctgca
     5461 atactttgat ctgctggcgc aggtaataat cgcgttgctg ctgatcaatg tctgtgttta
     5521 cttttttttg aatttcctgt ttcagttcca aaagtttcac ttctttaagc atgtactgca
     5581 aaagaagtgt cgctcgcttt tgtccatttt ctgcttccag taatttttgt ttatctgaaa
     5641 catccacatt cagatttgaa gaaagaaaat gtgtgaggaa accgggatca tgaatgttgt
     5701 cgagcgctat ttgtgcttcc tgtggaattt caggatttag ccgtaaaatt tttgaagcag
     5761 agtctttaat ggattgaaca agggcgttgg tttctttttg ccctcccgcc aggaacttgt
     5821 cttcaagaat tttaatggat acggtgtaat agggttcctc ttgaataatt gacttaattt
     5881 caaacctgtg acttccctga attattatag taatactacc gtcaggaagt accagcatct
     5941 tgataatttt cgccaccgtg cccactttat agagatcttt caaagcgggt tcctctgctt
     6001 tgctgttgct ttgggcaatt actccaatta tcctcttcga ctcgtacgct ttcttcacca
     6061 acctgataga tttctttctt cctacagtaa ttggtataac aactccgggg aagagaactg
     6121 tatttttcac cggcaataag gataattcat ccggaatgtc ttctttttta ggcgagttat
     6181 ctccactctc gttcacaatt tgtatatact caccatcatt gctgctttcc ggattgccaa
     6241 gcaagtgttt atttaaaaaa aagtgtattc tatccattaa aagatttctc tgtgccaaaa
     6301 tggcataaaa tatgccttta gcttcttaga ttattacttg ccattaaggg ttcaataggt
     6361 atgccaaaac cgggactgca tttggcgtat tcattgtgca ttatataaga aatgtagcca
     6421 atcatagata aatttgtgaa agcaccaact aaagtgtaat gacctcaggc aaaattataa
     6481 catttctact tttgatgttc acactgtcct atgagggatc ttttggtcag aggtctgtgg
     6541 agaagcggtt atttagtcct gaattcatta aaaaggatat ttccacttat tcccaatctg
     6601 atttattcga ctttccgaac attaatttgg ttccttttta ctaccacaga tccgaaatga
     6661 accgaattca aaaccttgaa aacagaaaga attggaaaga tttgtatccg gtgctgcgtg
     6721 catacgtgaa gaatttcgga atagaaaatt tttataaaga tacttattgg gtttggagac
     6781 ttgccaaatt gacagaagaa atgggaaacc atgatgaagc attgctgtta tacagactgg
     6841 ttttgaaaca tcatagagag gatattgaca taacgaaagt ggagcttcat tatgactctc
     6901 tgattgccaa tgataaagat tattttgtgc ctattgatta ctactatgaa ttggtagaat
     6961 tccgtaaaga ggtggatacc ttgaggccac cacggggagt attaataaac atgggaaaag
     7021 atattaacag tgaaacaagc gattacgcac caagtctgaa tatggcagac aaaatgttaa
     7081 tctttacgag caaaaggaaa gtaataaatc caggcatggg agaagaaccc aatgaagatt
     7141 tatttttttc catcaatgat gacggatact ggcgacctgc ccaatctttt cctgatttga
     7201 atacgcttta taatgaaggc tccgcctgca tgtcaaaaga tggaaaaact ttatacttta
     7261 gccgttgtta ctcacctgaa tcgtacggca attgtgacat ttttgtggca aagatgcaat
     7321 ttgatggcaa atggagtgat gtggccaata tgggtccgaa catcaatagc ctggcctggg
     7381 attctcatcc tagcttaaat catacggaag atacgctcta cttcgcttca gacaggattg
     7441 gcggttttgg tcttgcagat atttacttta cctataaaga tgcaaatggc agatggatgc
     7501 cggccaaaaa tgcagggccc attgttaatt ccagggccaa tgaaatttcg ccctttttcc
     7561 atcataagga tgaaatcctt tatttcagca gtaatgggca cgcggtaaat tttggcgctt
     7621 tcgatattta taaaacgcag agagtgcgga gcagttggag tgaaccaaaa aatattggac
     7681 cgctggtgaa cggttcagga aatgagtttt actttactat cgattccgaa tccaggaatc
     7741 tttattattc ccattccacc gaaaaagaca tcaataatct tgatttatat tcatttcctg
     7801 tacctatgga agcacaaccc ggagccacta caaccctgga gggaagtctt aatgatggat
     7861 cagatgagcc attcaaggga atagtttcta tcattgatat ggataacgga attgaagtgg
     7921 caccaaaatt tctgcgtccg gacggaactt tcgaattcaa tttaatcaac aataacaatt
     7981 acctgcttat tataaggggt gaagagttct taaagattga agaattattt ttcctggatg
     8041 ggaatactga atttctacaa acaactgaaa atattaaatc tactattgaa tttgagtcca
     8101 ttcaatttgg gaatgaagag tcaaacatca atggatccat gtttggtgat ttaaataaag
     8161 ttggcgaatt tcttctcgac aatcctgaat ttaccttaaa aatttccggc catacggatt
     8221 ctgacggaag ggaagaattt aaccttgaat tgtcacagaa acgggcggac gcgattaagg
     8281 aatatctggt ttacttcaac tcggttgagc ctgaaagaat tatttcggaa ggttatggaa
     8341 gctccaaacc actggttaaa gaagtaactg atgctgataa gagtgttaac cgcagggttg
     8401 agttccagct ggtaaaaggt ggaggataat actatccacc aaacaatccc ttaattgctt
     8461 ccagcttaaa aatatcattg aatattatga aagccattaa ggccagtaga aatacaacac
     8521 cgattttttg tgctccttcc agaaatttat ccgatggttt tcttcctgaa ataatttcat
     8581 aggaaagaaa cataacatga cctccatcta aagcaggaat gggtaagaaa ttcatgaagg
     8641 caagtaccat ggaaagcaaa ccggttatac tccagaacct tgcccagtcc caggttccgc
     8701 caaaaatttg tgcaatacca atcgggccgc tgattgattt actggcagac acttcacctc
     8761 taaaaatttt cccaagcgct tttatattca cccaaacggc cataaatgcc ctgccggttc
     8821 cttccggcac tgattcaaaa aagctatagt ttttatgact aaaatcaatt atggcctcgg
     8881 tgtaaaatcc aatcgtacct tcttcattta tttttaccgt aagattaaaa aggtcgcccg
     8941 ctctttctac aaccaccgga acgtcttcac cgacatgttg atcttttatg ctttcaaatt
     9001 ggtccctgta aagtgttgat tcgcctgcca tttcaataat tttatccccg ggcctgaggc
     9061 ctgacgtcag ggcatcgcta tcttccggga tgctggctat cctccaggga taacgaatct
     9121 caataaattg ctgcgcggct tttctatcgg aaagcttttc gataaaatcg atgggtatgg
     9181 gtatatttac taacttaccg tctctttcta ccgtgtagta accgccattg ctcaacaaaa
     9241 catttgggct ttgaatatca ccaaagcttt ggtagtcttc tccattgatt ttaacaatct
     9301 tatccccggt ttcaaaacct aattccctgc ctatttcgag tgcaacaatt ccatacttat
     9361 tggcatcttc cttagaaatg aaagtatcac cataatacca aacaatcccg atgctaataa
     9421 atattcccag aaaaacatta accgttattc ctcccatcat cacgataagc ctttgccagg
     9481 ccggctttga gcggaattcg taatcttttg gttcctcttt aaggtgattg gtatcaaatg
     9541 attcatcaat catacctgag atttttacat atccaccgag aggtatggca ccaatcgagt
     9601 attccgtttc gccgtacttc actccatata tttttggtgg aaaacctatt gaatattgtt
     9661 ccacccgcat tccaaaagct ttagcagcaa cgaggtgtcc caattcatgt aatccaacta
     9721 atatagaaag gctcagaagt atctgagcgg tcattatcaa tccttccatt attttattat
     9781 ctctctggct ttaattcttg tttctttatc ggtgttaaca aaatcctcgt aacctggttt
     9841 aggaataaac ggtacttttt ccatacaggt ttccacaacc tctgacattc gcaaaaaccc
     9901 aattttatca tttaaaaaat tctccaccgc tatttcattc gccgcattca ggatacaagg
     9961 catatttccc ccaaaattaa tcgatttata ggccaattcc agattacgga atgtttttaa
    10021 gtccggcttc tcaaaagtca gcgtgggata attctcaaaa ctgtacctgg gaaagtctga
    10081 ttttagtcta ttcggataag ataaggcata aagtatcggt aacctcatat caggcacgcc
    10141 catttgcgcc tttatcgatc catcctgaaa ctgcaccata gaatgaataa tgctttgcgg
    10201 atgaataacc acctccacct ggccggcatc aagaccgaat aaccatttcg cctcaattac
    10261 ttccaggcct ttgttcatca gagtcgcgga atcgatggtg atttttgccc ccatgtccca
    10321 gtttggatgt tttaaggctt gttccttggt aacggaggtc aggaattctt gcgactttcc
    10381 cctgaaaggc ccgcctgaag cggtgagaat aattttctca ataggattat gaaactctcc
    10441 cactatgcat tgaaaaatgg ctgaatgttc agaatccact ggaaaaatat ttacaccggc
    10501 ttcccgcgca aggcttgtaa tccattctcc agctacaacc aaagtttctt tatttgcaag
    10561 cgcgatttcc tttccggctt ttatggcatt taccgttgga agtaaccctg agtacccaac
    10621 cagggctgtt agcaccatat caatagtctc catttccaca atggaagcaa gacttttaac
    10681 tccggaaaat actttaatat ttaatccagt cagttcattt tttacaaatt catattcatt
    10741 gtcattcaca attaccaccg cattcggttt gaatcgcttt gattgttcaa ccaataactc
    10801 gctattgttg ttggaagtaa gaacctccac ctgaaactga tccgggtgct ttccaattac
    10861 ttccagggtt tgtcttccaa ttgagccagt acttcccagg attgcgatat ttttttttat
    10921 tgaatccaaa tcaagattaa atgatggcgt attttattaa ttcgtcggca atttttcgat
    10981 cattagaaag tcgtggtact ttattttgtc cgcctagttt tccaatttgt ttcatatatt
    11041 ctctgaatga atccctgcgg agcgaaatta ttttaagatt agaaagaatc ttccctgaga
    11101 ttaaatcgtc atagtaagga tttaattcct ggagccgtaa atttaactca taaatgaatt
    11161 tagacaaatc gacaggcgga ctccggaact caatatacca ttcgtggtgc ggcagaccgc
    11221 tttccggaga aacattcgga gcgacagtaa attcgataat ttctgttcca ggctgagatg
    11281 agcatgcatg ttccattgct ttttcaactt cctccccaat aacatgctca ccaaatgcag
    11341 aaataaaatg ctttatacgg cctgtaacta ccagtctgaa cggactatcg gagataaatt
    11401 tcactgtgtc acccagagaa taagaccaaa gaccggcatt attgctgagc accacagcat
    11461 aattcttatt aagttcaatt tgatcgatca ttaacctgga aggtttttca ttaaaaaatt
    11521 catcagccgg aataaattcg aaaaagattc ctgaatttaa taaaagtaaa agtccgttct
    11581 tattctgaga atcctggtag gcaatgaagc cttctgaggc cgggtaagtt tcaatggaat
    11641 cgatttcttt accaatggat tcaaaaagtt ttttccggta cggctcaaaa cttactcctc
    11701 catagataaa tagtgaaaaa tcagggaata catctttaat caatttttga gttctgtcat
    11761 gaattcgatc aaaatacatc tgtacccagg ggggaatccc ggaaatcaaa ctcataggtt
    11821 gagccaaagt ttcatcaatt attctttcga gtttttcttc ccagtcttcg atgcaatttg
    11881 tttcaatgga aggcagctga ttgccacgaa gccatgaagg aacatgatga tttacaatgc
    11941 ctgaaagccg gccagttgga attttaccgg tgaaatcaag ctgcggactg ccagagagaa
    12001 aaatcaattt cttatcgctg aaagaacttt ttcctgtttc atgaatataa gataagatgg
    12061 cgtttcttgc tccgttaatg tgattgggaa tactttcttt cgtaagagga atatatttaa
    12121 cacctgaggt tgtgccggaa gttttagata agtatttcgg aattcccggc cagagaacat
    12181 tgcgctcacc ttttacaatt ttctccatat agattttcag gtcttcataa tccctgacag
    12241 gaacctgcga tttaaaatca ttgtaattct ttattgaatc aaaattgtgc tgcttaccaa
    12301 aaagagtttg ggaaccggta ctgattaaat tttcaaaaat ctttttttga ctttcggcgg
    12361 gtgctgagga ccaagcgata gtttcttttg caacgtatgc ggcaaagggt tttgcaagaa
    12421 tcgaacgaat gcccatggct gcaaatctac cccaaacatt gaaatagaaa gaatgggcga
    12481 atcaaagatg tattacacta taatataatt attttattat aattttatct ttatataagt
    12541 aaaatatgaa aaaatatacc ttaccctttg ttatactttg tttaatttat ttgttttcct
    12601 gcgatccaaa agataaacca tcagttgaag ttgagga
//
LOCUS       DASWHX010000084         5326 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_2292891, whole genome shotgun sequence.
ACCESSION   DASWHX010000084 DASWHX010000000
VERSION     DASWHX010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 5326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5326
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2292891"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..394
                     /locus_tag="VGA21_05855"
     CDS             <1..394
                     /locus_tag="VGA21_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017732400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEX9650064.1"
                     /translation="MNPEPLDYNNMPGCTYCSPEIASVGYTEKAAKEAGYEIKVGKFP
                     FSASGKASAAGAKEGFVKVIFDAKYGEWLGCHMIGNNVTEIIAEAVVARKLETTGHEI
                     IKSVHPHPTMSEAVMEATAAAYDEVIHI"
     gene            613..686
                     /locus_tag="VGA21_05860"
     tRNA            613..686
                     /locus_tag="VGA21_05860"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:647..649,aa:Pro,seq:cgg)
     gene            728..1273
                     /locus_tag="VGA21_05865"
     CDS             728..1273
                     /locus_tag="VGA21_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002700005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX9650065.1"
                     /translation="MNRKSIRLKGYDYSQAGLYFITICCQDRACLFGDVVNGKIGLNV
                     AGKIANECWLEIPEHFPNVILHEYTVMPNHIHGIIELTEIVGAKNFSLHQHSPHHTGA
                     NDFSPLRIPFVSPSRTIGSIVRGFKIGVTKWMRQNTDVETVWQRNYHEHIIRNEQSYQ
                     TISDYIINNPGKWADDKFNLE"
     gene            1345..2271
                     /locus_tag="VGA21_05870"
     CDS             1345..2271
                     /locus_tag="VGA21_05870"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEX9650066.1"
                     /translation="MHHKIHSLKMRYFSAFYDDAFSKVIPQLRTESWKALFIMGSGRN
                     GSTLFSCILNNHNKICIPPEHNGIPAAIRYWHTHPFQSWTNKVNHITSIFEKSALWNF
                     EGEKTKSELSGIPVNQRNINEIINSIYGYFGRKQGKLNPVWGDKTPSNTHFIKTIKKQ
                     FPLSKIIFLVRDPRDVVSSLLEINEKHFLKTFDFLLWRWIDSIDKYRFLSKKFPEDIR
                     MLKYENLVSNPSEEVTGIIRWLDLEPQPGLLNSYAANMEFLGIKDVEHHKNINNPIST
                     GSIGKWKYNLAKESVTKIHRRLGAAMKQLGYI"
     gene            complement(2448..2834)
                     /locus_tag="VGA21_05875"
     CDS             complement(2448..2834)
                     /locus_tag="VGA21_05875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650067.1"
                     /translation="MKNPLNLLLAAALLLISGCKDDEPANAVLLGAWENTGLLVTECT
                     DPANNYTQDCQDNCEIIFFTETTVISLGETFPYTVSENNLNIYENSYTISVLQYSIEN
                     TVLTLTFKADSADGSCKFASKYVKIP"
     gene            complement(2930..>5326)
                     /locus_tag="VGA21_05880"
     CDS             complement(2930..>5326)
                     /locus_tag="VGA21_05880"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9650068.1"
                     /translation="SYFLSTIRTFRKYRVFTFINLIGITLGMSSFILIMKYVSNELSY
                     DQSHTKKDRIYRVQLDRYNQGELSTQWAAGCAGVGPAMLESFPEVDRFVKMHSSNAMI
                     SFGEKVFREDHFFYASEEFFRVFDFKLLQGIDSLVLQEPFTAVLSASTAKKYFGEEDP
                     LGKTIIHNGSNSFRITGIFEDVPENSHMKFDCLLSFETYISLTSPQARNSFDWDGFYT
                     YVLLKEGADGKFLESKLPSMVNQYAGETMEEFGMNMIFHLQPLTDIHLNSNYMVEFEA
                     NGDLQIVYFLSIVSFFILIIAWVNYINLSTSMAINRMKEIGIRKIIGAQRSQLITQFV
                     FESFILNLFAIFFSVGLAYLLLPELNEIAGRRLILFNGTDLDLWGKVLLLFVAGGIVA
                     GIYPAFVISSFKPSQVLKGRSTKSSAGNIMRRFLVVFQFAISVILIIGTFVVFHQLQF
                     LKQQELGMNIDQTLVIVGPNVTDSTFISKHENFKVEASRLPYVSAITASTTVPGSQPP
                     WNAGGIRKLEDDPVKGQQYRIIGMDEDYIDYYGLSILEGRKFSADYSNESQSVIFNES
                     AIKLMGFEMMEDAINRKIYFWGDTFAIVGVVKDFHQESLKKSTEPLILRYFSNISSFY
                     SIKVSAGHYQKAVTGMEELFKTFFPGNEYDYFFLDDHFDQQYKSELLFGKIFSSFSTL
                     SIVIACLGLFGLTGFVTTLRTREIGIRKTLGASRNELIILLSTEFSKLVLMAWIPAIP
                     LVWWLSNRWLADFAYKVNLSWWLFAGPGLLILMIALATISYHTLKISSLNPTETLRTE
                     "
BASE COUNT         1726 a         1023 c         1081 g         1496 t
ORIGIN      
        1 aatgaacccg gaacctttag actataataa catgcctggt tgtacatatt gcagccccga
       61 gattgcatct gtgggttata cggaaaaggc cgcaaaggaa gccggctatg aaatcaaagt
      121 tggcaaattc ccattttccg catcgggcaa ggcaagtgcg gccggggcca aggaaggatt
      181 tgtaaaagtg attttcgatg cgaaatacgg ggaatggctg ggctgccaca tgattggcaa
      241 caacgtgact gaaataatag ccgaagccgt tgtcgccagg aaactggaaa caacaggcca
      301 cgaaattatt aaatcggttc atccgcatcc aactatgtcg gaagctgtta tggaagccac
      361 tgcagcggca tatgatgagg tgatacatat ataaggaagt taagagttgt gagtattgag
      421 tgcggagaaa ctcgctcata atgtagtcaa gagttgagag tttagagtgc ggagaaactc
      481 gctcataatg tagtcaagag ttgagagttt agagtgttga gaaactcgct gataatgtag
      541 tcaagagttg agagtttaga gtgttgagaa actctctact ctatactcaa aattctatac
      601 tcttaactca atcgggatgt ggcgcagctt ggtagcgcgc gtcgttcggg acgacgaggc
      661 cgctggttcg aatccagtca tcccgacttt gttatttcat ggattttgat ttaataaatt
      721 gatcaaggtg aaccgtaaat ctatccggtt aaaaggatac gattattcgc aggcgggttt
      781 gtattttatc accatatgtt gtcaggacag ggcgtgtttg tttggtgatg tggtgaatgg
      841 caaaatagga ttaaacgttg cggggaaaat tgccaatgaa tgttggttgg aaataccgga
      901 acattttccg aatgtgattt tgcacgaata tactgtaatg ccgaaccaca tccacggtat
      961 tattgaattg accgaaatcg taggggcgaa aaatttttcg ctccatcaac attcgcccca
     1021 tcatacaggg gcgaatgatt tttcgcccct acgaatacca tttgtttcac cgtcacgaac
     1081 aataggttcc attgtacgcg gatttaaaat tggtgtaaca aaatggatgc gtcaaaacac
     1141 ggatgttgaa accgtttggc aacgcaatta ccacgaacac attattcgta atgaacaatc
     1201 gtatcaaacc atttcggatt acatcataaa taaccctgga aaatgggcag atgataaatt
     1261 taatctggaa tgaaattcac cgagggaaaa ttagaaaagg cattttccga gttgtctgtg
     1321 gagaaaaaat cttaaattca aaaaatgcat cacaaaatac acagtcttaa aatgcgttat
     1381 ttttctgcct tttatgatga tgctttttct aaagtaatac cccaactccg aaccgaatcg
     1441 tggaaagcat tattcattat gggaagtggc cggaatggaa gcacattgtt ctcatgtatc
     1501 ctaaacaacc acaataagat atgtattcct ccggagcata atggaattcc ggcagccatt
     1561 cgctattggc acactcatcc atttcaatcc tggacgaata aggtaaatca tattacttcg
     1621 atatttgaaa aatctgcctt atggaatttt gaaggtgaaa aaactaaaag tgaattatct
     1681 ggaattccgg ttaaccaaag aaatatcaat gaaatcatta attccattta tggttatttt
     1741 ggaagaaagc aagggaagtt aaatccggtg tggggtgata aaacgccttc aaatactcat
     1801 ttcattaaaa caataaagaa acaatttcct ttaagcaaaa ttattttttt agttcgagac
     1861 ccacgagatg tagtttcatc tttactggag ataaatgaaa aacactttct caaaacattc
     1921 gacttcttat tgtggagatg gatagattcg attgacaaat acaggttctt atcaaaaaag
     1981 ttcccggaag atattaggat gctgaagtat gaaaatctgg tgagtaatcc aagtgaagaa
     2041 gtgaccggta taatcagatg gcttgatctg gaacctcaac ctggtttgct taatagttat
     2101 gcagccaata tggaattcct gggaattaaa gatgttgagc atcacaaaaa tataaacaat
     2161 ccaatttcga caggttctat aggaaagtgg aaatacaatc ttgcaaaaga atcggtgact
     2221 aagatccaca gaagattagg tgctgcaatg aagcaattgg gatacattta atctcgagta
     2281 ttgtggctat ttgccaatta tacgttaata tttcatttct ctaaaatgaa taagcttgtg
     2341 gccagggttt ttgcagtagt taaccgaggt ataccttata agaaacaaac taccaaaatc
     2401 agttggtttt atcatagtaa tcaagggcga gaaaagaaat ctctgcctta aggaattttg
     2461 acgtacttag atgcaaactt gcaactacca tccgccgaat cagccttgaa tgttagtgta
     2521 agcacagtat tttcaattga atactggaga acggagatag tgtaggaatt ttcgtaaata
     2581 ttcaaattat tctcacttac cgtataagga aaagtttctc ccaacgatat tacagttgtt
     2641 tcagtgaaaa aaatgatttc acaattatcc tggcaatctt gcgtgtagtt attggcaggg
     2701 tcagtgcatt ccgtaactaa aaggccggta ttctcccagg ctccgagtaa gactgcattg
     2761 gctggttcat catctttaca accggaaatc aaaagcaatg ccgcagccaa caaaagattt
     2821 agaggatttt tcatataata caattttgta ttgtaaaaat acaacatttt atcaactaat
     2881 aatagattga ttataaatcc caagtgagtt aaacaaaaag ggaaaacctt tattcagtcc
     2941 gcaaagtttc agttggattg aggctggaaa tcttcagcgt atgatagctg atggttgcca
     3001 aagcaatcat caaaatcaat aatcccggac cggcgaacaa ccaccagctt aggtttactt
     3061 tgtaagcgaa atctgccaac cacctgtttg aaagccacca caccaaaggg attgcaggaa
     3121 tccaggccat taagacaagt ttcgaaaatt ccgtagaaag taaaatgata agttcgttcc
     3181 gggaggcgcc caatgttttt ctgattccaa tctcgcgggt tcttagggtt gtaacaaagc
     3241 cggtgagtcc gaataaaccc agacaggcaa tcacaatgga cagtgttgag aaggaactga
     3301 aaattttccc aaatagtaat tccgatttgt attgctgatc gaaatgatca tcaagaaaaa
     3361 agtaatcata ttcatttccc ggaaagaacg tcttaaaaag ttcttccata ccggtaacgg
     3421 ctttctgata atggccagcg ctaactttaa tggaatagaa actggagata ttggaaaagt
     3481 agcgcaagat taaaggctct gtacttttct taagcgattc ctggtgaaaa tcttttacca
     3541 cgccaacaat ggcaaacgta tcgccccaga aatatatctt ccggttaatg gcatcttcca
     3601 tcatttcgaa tcccatcaat ttgattgcag actcgttgaa aatcaccgat tgactttcat
     3661 tggagtaatc agcggaaaat tttcttcctt ccagaataga taaaccatag taatctatat
     3721 agtcctcgtc cattcctatt atacggtatt gctgtccttt gaccgggtca tcttcaagct
     3781 tcctgattcc gccggcattc cagggcggct ggctgcccgg aactgtagta gatgctgtga
     3841 tcgctgaaac ataaggcagc cttgaagctt ccactttgaa attctcgtgt tttgaaataa
     3901 aagtagagtc ggtaacattc ggacccacaa taaccagtgt ctggtcaata ttcatcccca
     3961 attcctgttg tttcaggaat tgaagctggt ggaaaacaac gaatgttccg ataatcagaa
     4021 tcacggaaat ggcaaactga aatactacaa gaaagcgtct cataatattt cccgcagaag
     4081 atttggtact tcttcctttg agaacctgcg aaggcttgaa tgaagaaata acgaaggcag
     4141 gataaatgcc ggcaacaatt cccccggcga caaataacag taataccttt ccccacaagt
     4201 ctaaatccgt tccgttaaat aaaatcagcc ttcttcctgc aatttcattt aactcaggca
     4261 ataacaggta agctaatccc actgagaaaa aaatcgcaaa aagattcaga atgaatgatt
     4321 cgaaaacaaa ttgggttatc aattgcgagc gttgcgctcc gattattttt ctaatcccga
     4381 tttccttcat tctgtttata gccattgaag tggataaatt gatgtaattc acccaggcaa
     4441 taattaggat aaagaatgat acgattgaaa ggaagtaaac aatttgcagg tcgccatttg
     4501 cttcaaactc gaccatgtaa tttgagttca agtgaatatc agtcagaggt tgcagatgaa
     4561 aaatcatatt cataccaaat tcttccattg tttcgcctgc atactgatta accattgagg
     4621 gcaattttga ttcaaggaat tttccatctg ctccttcttt cagcagcaca tacgtataaa
     4681 aaccatccca atcaaatgaa ttccgcgcct gtggggaagt aagacttata taggtctcaa
     4741 aactcagaag gcaatcaaac ttcatgtgcg aattttcagg tacgtcttca aaaatcccgg
     4801 taatcctgaa tgaatttgac ccgttatgaa taatggtttt tcccaatgga tcctcttctc
     4861 caaaatattt ttttgccgtt gatgccgaca aaacagcggt gaatggttct tgcagcacca
     4921 aagaatcaat tccctggagt aatttgaaat caaaaactct gaagaattcc tcgctggcat
     4981 agaagaaatg atcttcgcga aatacctttt caccaaatga aatcatagca ttgctgctat
     5041 gcattttcac aaagcggtca acttcaggaa acgactcaag catggccggt cccacaccgg
     5101 cacagccggc ggcccattga gtagacaact ctccctggtt ataacggtct agttgtacgc
     5161 ggtagattct gtctttctta gtgtgactct gatcatagct taactcatta gagacatatt
     5221 tcataatcag aatgaaagaa gacattccca gtgtaattcc tatcaaatta atgaaagtaa
     5281 aaacacggta tttacggaat gtccttattg tggagagaaa atagct
//
LOCUS       DASWHX010000085         2595 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_3383762, whole genome shotgun sequence.
ACCESSION   DASWHX010000085 DASWHX010000000
VERSION     DASWHX010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 2595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2595
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3383762"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1291
                     /locus_tag="VGA21_05885"
     CDS             <1..1291
                     /locus_tag="VGA21_05885"
                     /inference="COORDINATES: protein motif:HMM:NF013715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HEX9650069.1"
                     /translation="SDTALYIAIGIIGATVMPHNLYLHSSLVQTRRIDNSDKGLWDAI
                     KFNIIDSAIALNMAFFVNAAILVLAAATFYEIGLYQVEEIQDAHKLLAPLLGTVWAAI
                     LFAVALIAAGQSSTITGTLAGQIVMEGYLNLRIQPWLRRLITRLIAIIPAFLVILYYG
                     EEKTGALLILSQVILSLQLGFAIIPLIHLVSDKERMGKFVIKFGTRIASWVIATIIVT
                     LNVKLVIDEVSGWITTSENPIIILLTVVPLVVGAGILLLYITFKPVLVKRKARKIKVP
                     HGTVQEISISTTQMYKRIAISIDFSSIDGEAINSAINQGGKEADYLLIHINESAGAIM
                     MGSEIDDYESGSDKANLKKYAEQLRGQGYRIETKVGYGTPKKKIPELVKEYNADLLVM
                     GAHGHKLIKDLIFGTTVDAVRHRIDIPLFIVKEAKKK"
     gene            1304..>2595
                     /gene="mnmE"
                     /locus_tag="VGA21_05890"
     CDS             1304..>2595
                     /gene="mnmE"
                     /locus_tag="VGA21_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014022278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEX9650070.1"
                     /translation="MNPQSIVYSDDTIIALATPPGVGAISVVRLSGKYSISVVNKVFQ
                     GKNLEAEKSHTIHLGQIKDEDKIIDEVLVSIFREPNSYTKENVVEISCHGSNYIIKEI
                     ISLFIRLGIRFARPGEFTQRAFINGRFDLVQAEAVADLIASDSDTSHEMAMKQMRGGF
                     SKEINTLRESLIHFASLIELELDFGEEDVQFASRKELKETVLTLKSEIEKLISSFRLG
                     NAIKNGVPTVIVGKPNVGKSTLLNALLKEDRAIVSEIPGTTRDFIEDEIFIEGIRFRF
                     IDTAGLRDTEDAVEAMGVARTREKMQKASLILYLFDLSKEKLVEIEKVVAELESQKIP
                     FLKIGNKKDIAAPALLKEFGNDEEMILISAATEENLEELKTKILHKVHLDNINTGESI
                     VTNVRHLEALRKTSSDLDEVLTGLEKNITHELLAQDIR"
BASE COUNT          803 a          475 c          595 g          722 t
ORIGIN      
        1 cagcgacacc gctctatata tcgccattgg aattattgga gctacagtga tgccacataa
       61 cctttactta cattcatcgc ttgttcaaac ccgccggatt gacaattctg acaaaggatt
      121 gtgggatgca atcaagttca atattataga ttctgctatt gcccttaaca tggcattttt
      181 tgtaaatgca gcgatacttg tgcttgcggc tgccactttc tatgaaattg gtttgtacca
      241 ggtggaagaa atccaggatg cgcacaaatt actcgcaccg ctgcttggaa cagtatgggc
      301 agcgatactt tttgccgtcg cgcttattgc agcgggtcaa agttctacca tcaccggaac
      361 ccttgccggg cagatcgtca tggaagggta tcttaatttg cgaatccagc cctggctgcg
      421 aaggttaatc acccggttaa ttgcaattat cccggcattc ctggtcattt tatattacgg
      481 tgaggaaaaa accggggcat tacttattct cagtcaggtg atcctgagtt tgcagttggg
      541 ttttgcaata atccccctga ttcatttggt gagtgacaag gaaagaatgg gcaagtttgt
      601 aataaaattc ggtacaagga tcgcatcctg ggtaattgca acaatcatag taaccctgaa
      661 tgtaaaactc gtcatcgatg aagtttcggg ttggataacc acttcagaaa accctatcat
      721 catcttgcta acagtagtcc ctttagttgt tggggcagga attcttttgt tatatattac
      781 atttaagcca gttttagtga agagaaaagc cagaaaaata aaagttcctc atggtacggt
      841 tcaggagata agtatttcta ctacacaaat gtataaacga atagcaatca gcattgattt
      901 ttcttctatt gatggagaag caatcaacag tgctataaat cagggtggta aagaggctga
      961 ttatttactg atacatatta atgagtcagc cggcgcgatt atgatgggca gtgaaattga
     1021 tgattatgag tcaggatctg ataaagccaa tctgaagaaa tatgcggaac aattgcgtgg
     1081 acagggatat agaattgaaa ccaaagttgg ttacggtaca ccaaagaaga agattccgga
     1141 attagtgaaa gagtataatg ctgatttact cgtaatggga gctcatggtc ataaattaat
     1201 taaagacttg atttttggta ccaccgtgga tgctgtccgg catagaattg acattccact
     1261 attcattgtt aaggaggcaa agaaaaaata aataacatcg gggttgaatc cacaaagcat
     1321 tgtttatagt gatgatacta tcatagccct tgcaactcct ccgggagttg gcgctatcag
     1381 cgtggttcgg ttatccggca aatactccat ctccgtagtg aacaaggtat ttcagggaaa
     1441 gaatctagag gccgaaaaat ctcatactat tcacctgggg cagataaagg atgaagataa
     1501 aattattgat gaagttttag taagtatttt cagggaaccg aattcctata ccaaagagaa
     1561 tgtggtggaa atctcctgtc atggctccaa ttatattatt aaagaaatta ttagtttgtt
     1621 tatccgactg ggtatccgat ttgcaaggcc gggtgaattt acccagcggg catttattaa
     1681 cgggagattt gatttggtac aggcagaggc tgttgctgat ttaattgctt ctgattcaga
     1741 cacctcccat gaaatggcga tgaaacaaat gcgtggcggt ttcagtaagg agataaatac
     1801 tttgcgggaa agtttaattc atttcgcttc cttaattgaa ttggagcttg atttcggtga
     1861 agaggatgta cagttcgcca gcaggaaaga actgaaggaa acggtactaa cacttaaaag
     1921 tgaaattgaa aaattgattt ccagttttag gctgggtaac gcgattaaga acggagtacc
     1981 caccgtgatt gttggcaagc caaatgttgg taaatctacg cttttgaatg cgctgttgaa
     2041 ggaagacagg gcaatcgtaa gcgaaattcc cggaacaaca cgggatttta ttgaagatga
     2101 aattttcatc gagggaatcc ggttccggtt tattgacacc gccggcttaa gagataccga
     2161 agacgcagtc gaagcgatgg gtgtggccag aacccgggaa aaaatgcaaa aggcttccct
     2221 aattctctat ctgtttgatt tatcaaaaga aaagttagtt gagattgaaa aagtggtagc
     2281 cgaacttgaa tctcaaaaaa taccttttct gaaaattggt aacaaaaaag atatcgccgc
     2341 ccctgccctt ttaaaagaat tcgggaatga tgaagaaatg attttgattt ctgccgcaac
     2401 ggaggagaac ctggaagaat tgaaaacaaa gattctgcat aaagtgcacc tggataatat
     2461 caataccgga gaaagcatcg ttaccaatgt tcgccacctt gaagccttac gcaaaacatc
     2521 ttcagacctg gatgaagttt taacaggcct tgaaaagaat atcacccatg agctgttggc
     2581 ccaggacatt cgtca
//
LOCUS       DASWHX010000086        10479 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_2658920, whole genome shotgun sequence.
ACCESSION   DASWHX010000086 DASWHX010000000
VERSION     DASWHX010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 10479)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10479)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10479
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2658920"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            73..5559
                     /locus_tag="VGA21_05895"
     CDS             73..5559
                     /locus_tag="VGA21_05895"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEX9650071.1"
                     /translation="MDPLTVYYMQISDGGLDLNDQSLTVDVIDIYGSSSLFLTSSQVN
                     VAQSWGMYTNLATFDAGTSTIVMGGYNFNGGNQTYNNLLVSNSGNELYLYGSNAISSL
                     AMDPGAAVRFDAAQIQTLGSFSAAGNSTDLIFIGSTSDGITSTLNVATPVSGDWLIIQ
                     DNTAIGGGPFNANNSINLGNNTGWIFTAPVANNYYWFGGSGNWSDTNHWATLSGGPTL
                     HSVPPTRFDNVYFDGNSFPSGGTVTLDLPVNTCANMVWTGVTNNPSIVSPSSAEFHLY
                     GDITFSSEVTKDLRYVKLGGYDAISGYIIETGGTTFGNNSTIDFQGTGTYFLNNDLLA
                     GTIRFNNGILYTGSHDITANSFIIFGGNFIDLADSRVEVRFFQNFAFLTLSGNFETIA
                     IDGSQFGGQFYGGNQYYNKLELTGMVSVDGDNSYGEFVVNPGSTITLQSFALQSADQF
                     ILTGTRPLPIIIKSDSPGDQAFLGQSSGTVTADYVVLKDNDASGGAAFIATNAIDNGN
                     NLGWTFNSFLTSLSYFWVGGSGDWSDSNHWATSTGGSVFHNSGPGLLDNVYFDQNSFD
                     APGQTVTIDQIAYCFDMNWANLPSANNPRLLNNGYPINIVGSLYLSENMNIGAMDFYF
                     VATQLNEEIIIANADVSGLSFIFEGSGSWDIYDSFTLSSIDFNSGNLTFHDINLDVTS
                     FFIGSSDPKTLNLNSSSISTWFWQNFSGNLAFNAGNSDIKISRPGSFFGTFRGGFNAE
                     QYYSVTLDRNSSLNGDNYITNLYFIPNSNIRIQAFTTQNVNFISANGNEDQIISIFSS
                     LEGDQANFSAPPTSSFTVDYVHLMDNNATGGGFFNATNSSDKGNVTGWNGLLQWQEIT
                     FDPLNDNAFGNSVALNGSSTSSNAVTYSVFTGAGSVTGSSLNLDSQGLVGIKASQSGN
                     STYGPAKTVFQYIYAYNSPYELGTFKQANFVLGQPDFFTQDVVNSSSVFPGVSSAVVS
                     SNKQLAIAQTGRGRVVLYNSVPNSSGTPGDVILGKPDFTSTNSSTTDRDMNFSRWVAF
                     SPDGSKLVVSDGNRILIWNSIPAVNYTPADVVVGQSGFSTNDPGLSDTQFNGVWGIDI
                     SSDGKLFVVDANNNRVLIFNSIPTENNAAADVVIGADDFFTDSGGTSSTQLLFPIDVA
                     VSEQGQLFISDFQNDRVMVYNSVPTTNGAAADLVLGQPDFNTAQQLDPSIDNFNGPLG
                     VSTFRDQYLAISDFNNHRVMIYDLENLTPANIILGQPELFLNEPFNGGISGRSIINPY
                     QVHFDPAGNLLVSGASMHRVMFFGAPDINPPTIQNQTSNTYSLMSGESISANVTDDRA
                     NLTVYTEISGISQSTSSTIDLVDNGSTFSVPISFLDNSSEAFGIEYTLIASDGYNTTR
                     DGPYYIYLESNQNDRLSTISSTNLPEGTQESNYRIISIPLILDNPALTAVFGDELGNY
                     DPKNWRMFSWNPSGAGSYSELNDGSQLQIGTGYFLLIRSRPSGTVNFGNGSVPQVNRS
                     SPFTKSLSTGWNLIGNPYPFNIAWADILTTSGLASLDGKIYTSNGFQNITTLAAYSGA
                     FVMAPAATTLVFPISSGGEMPNPGNEGQRVQDQNDWQVQFSLRSGEFHNDYSGIGMHE
                     EADFSGDYFDGYTPPRFTAYLEMNSNHPESKYKIAKDIVPVENNHVWTYNLETNVPDD
                     KVKLSWDLGGFDPGEKQLIMMDISNNKIIDMTSRSEFEYPYVDNKQIKIFYGTGDFIA
                     EQLSPEKPYLSNYPNPFIGQTTISFGLPGSENDQFRVRFEVFDITGQMVTSIAEGVYM
                     SGYHELTLNKAQLNIQLKSGIYLLKMLVSGNSANYSSSLRLMIK"
     gene            5618..6790
                     /locus_tag="VGA21_05900"
     CDS             5618..6790
                     /locus_tag="VGA21_05900"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HEX9650072.1"
                     /translation="MVKRLLIFSASVAVSLNAWSQEIKGKSEPLYVNFQPATVIAAIP
                     VITWITPIDQITTLKIKKGSVKVGVNSDIKLKNVTLYINNLPTANDRGLSVSQPQDAG
                     YDEIFEKEVSLNDGDNEIKIVAEDITGKVTVESRILRVELPALMARNDYALLFATDEY
                     DTWGDLINPVNDATTIGKELEEAYGFKVEVIRNPTMTGVLTKLREYAKKSYQETDQLF
                     IFIAGHGQFDPFFNQGYLVCKDSRKDDEVKATYLSHSNLREIVNNIPSKHVFLAIDAC
                     FGGTFDPVIAKAGHRGGDDMYSELSSKEYIERKLRFKTRRYLTSGGKEYVPDGRPGRH
                     SPFASKMLEALRNYGGSDKIITLPELMGFIEKINPEPRTGSFGDDEPGSDFVFVAK"
     gene            6792..8156
                     /locus_tag="VGA21_05905"
     CDS             6792..8156
                     /locus_tag="VGA21_05905"
                     /inference="COORDINATES: protein motif:HMM:NF012340.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HEX9650073.1"
                     /translation="MIRNIVLGAAMLVASPILAQRHGTGLSFDDNAYQNSQAKAILTR
                     GDYQAVPSAASLKQYCPPAGNQLQLNTSVGWAVSYGAMTILKAKQNNWTDKHKIQQNA
                     FSPIYSYYKVKPENDIDCSAPISLDKALNSLKNEGAPKFVEFLEFCPNDIDNSQVPQK
                     QSVTGFNRLYDINAASDFKTSAVKKAIAEGYPVVVGMYVSPSFNTAKEFWQPREKFDS
                     KFPGLALCVVGYDDNKYGGAFEVMNSWSSSWGNEGFIWIPYNYFNEFSRYAFEIYDVP
                     EKTNADLGGALSFQLPDKSMMPVSLINNEGDYKISKNYGLGTVFRIIISNNATAFVYA
                     LGTDISGNVFQIFPMEGISPVLPYKSNNVPLPSEEDVIEVTEASGRDYVGIIYSREEL
                     PIQNIVSQMNSSSEAFPVRLRKLLGPRLVKGGEVNFGDSEINFQGKATNGSAVVITAE
                     LEYK"
     gene            8186..8608
                     /locus_tag="VGA21_05910"
     CDS             8186..8608
                     /locus_tag="VGA21_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013931176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX9650074.1"
                     /translation="MVIDKKKFLVISFVASGILFINSIFVVQESSFEESIKRGEIIYK
                     KDCITCHMENGEGIPGVFPPLAKSDFLLEHLDKSISAIINGLSGEISVNGQIYFGQME
                     PVKLTDQEIADVLNYSRNSWGNKANEIKAEEVAAQRTK"
     gene            complement(8609..9136)
                     /locus_tag="VGA21_05915"
     CDS             complement(8609..9136)
                     /locus_tag="VGA21_05915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650075.1"
                     /translation="MLKRQKFGWIIFILICSNLTPAQDIEFQLDEEGNAFYEDSVEVL
                     NQNLDSAIFRISTWASGQVTNEQDTLIFTQSSSSGILLKKILRISLIEEVDSNYSFGL
                     TLSVNLNSSVLRYKVYDIYHISLKTQQRCQNLGKAHGLCEFEGDDVGWSKVRIEMDRE
                     VRSILTNIEAFMSAK"
     gene            9236..9571
                     /locus_tag="VGA21_05920"
     CDS             9236..9571
                     /locus_tag="VGA21_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017730545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenosugar biosynthesis-associated
                     peroxidase-like protein"
                     /protein_id="HEX9650076.1"
                     /translation="MDKPYYKPEDLGKFGQITQWQEELGKKFFDYYNSVFEPGALTAR
                     EKALIALAVSHTVQCPYCIDAYTTASLENGADEKQMMEAVHVAAAIRSGSSLVHGVQM
                     MNKSREILM"
     gene            complement(9608..10150)
                     /locus_tag="VGA21_05925"
     CDS             complement(9608..10150)
                     /locus_tag="VGA21_05925"
                     /inference="COORDINATES: protein motif:HMM:NF025425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4251 domain-containing protein"
                     /protein_id="HEX9650077.1"
                     /translation="MRRFLIVIIVFIPFVAFPQEIRSDKKLTRQEKKEQRLQEQEAVE
                     AKIKEMVTNSEWVLEVTALLDERNNHGFLNTPNNFVAVKQKDGVVQLMLPGMRGWNGI
                     GGMTLRGKVENYEVKTSGKEIYATGTIFTNQGAFTFRLYPTIHKTKMVLSGSFNDTLT
                     LIGNLISPGETVLFEGINVY"
     gene            complement(10289..>10479)
                     /gene="ssrA"
                     /locus_tag="VGA21_05930"
     tmRNA           complement(10289..>10479)
                     /gene="ssrA"
                     /locus_tag="VGA21_05930"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT         3052 a         2120 c         2183 g         3124 t
ORIGIN      
        1 actcaatcta taacttccaa cggagcacaa ttgggcaact caatttatat tttttccggt
       61 aattattttc tgatggatcc gcttaccgtt tattatatgc agataagtga tggcggacta
      121 gatctgaatg atcaatcgct cacggttgac gtcatcgata tttatggatc atcctcttta
      181 tttctgactt cctcccaggt gaatgtagct cagtcatggg gcatgtatac taacctggct
      241 acttttgatg ccggaacaag tacgatagtg atgggagggt acaattttaa tgggggcaat
      301 caaacttata ataatctcct ggtttcaaac agcggaaacg aactttacct gtatggttct
      361 aatgcaattt cgtcattggc catggatcca ggtgctgctg ttcgatttga cgcagcgcaa
      421 attcagaccc tcggcagctt ttccgccgca ggtaatagca ccgatctcat ttttatcgga
      481 agtacttctg atggtattac ttcaaccctg aatgttgcca cacctgtttc cggcgattgg
      541 cttatcattc aggataatac ggccattggt ggtggcccct ttaatgccaa taactccatt
      601 aaccttggaa ataatacagg ctggattttc actgcacccg tggcgaataa ttactattgg
      661 tttggaggtt ccgggaattg gagcgataca aaccattggg caactttatc agggggaccc
      721 actcttcact ccgtccctcc tacccgtttt gataatgtat atttcgatgg taattccttt
      781 ccttcaggtg gtaccgttac ccttgacctg ccggtgaaca cctgcgcaaa tatggtttgg
      841 accggagtta caaataatcc atcaattgta tcaccgtcca gcgctgaatt tcatttgtat
      901 ggtgatatca cattttcatc agaggttaca aaagatttac gatatgttaa acttggaggt
      961 tatgatgcta tctcaggtta tattattgaa accggcggta ccacatttgg aaataattcg
     1021 accattgatt ttcagggcac aggcacttat tttctcaaca acgatctact tgctggaacc
     1081 atcagattta ataatggcat actttacacc ggcagtcatg atataactgc aaattctttc
     1141 attatttttg gcggcaattt cattgacctt gctgattcca gagttgaggt tcggtttttt
     1201 cagaatttcg cattcctgac attgtccgga aatttcgaaa ctattgccat tgatggcagt
     1261 cagtttggcg gccagtttta cggaggcaat caatactaca ataagctcga attaacgggc
     1321 atggttagtg tagatggaga taatagttac ggtgaatttg tagtaaatcc gggctcaact
     1381 attacacttc aaagttttgc gctgcagtcc gccgatcagt ttattttaac cggtacccgc
     1441 cctttaccca taatcataaa atcagattca cctggcgatc aggcattttt aggccaatcc
     1501 tccggaacag ttacggccga ttatgttgtc ctgaaagata atgacgcctc cggcggagca
     1561 gcttttattg caaccaatgc cattgacaat ggaaataatt taggctggac atttaattca
     1621 tttcttactt cattaagtta tttctgggtg ggcggaagcg gtgactggtc agacagcaac
     1681 cactgggcca ccagcaccgg tggatcggtt ttccacaatt caggtccggg tttattggat
     1741 aacgtttact ttgatcagaa ttcctttgat gcaccgggac agaccgtaac aatcgatcaa
     1801 attgcctatt gctttgacat gaactgggct aatctacctt cagcgaataa tccacggctt
     1861 cttaacaatg gctatcccat caacattgta ggttctttat atctttctga aaatatgaat
     1921 attggtgcta tggactttta ttttgtagca actcaactta atgaggaaat aattattgct
     1981 aatgccgatg tatctggcct ttcttttatt tttgaaggtt cgggtagttg ggacatttac
     2041 gattcattta ccttgtcttc tattgacttc aacagcggaa atctgacttt ccatgatatt
     2101 aatcttgatg ttacatcttt ctttataggg agcagtgatc ctaaaacttt gaatcttaat
     2161 tcttctagta tttctacctg gttttggcaa aacttcagtg gcaacctggc atttaatgca
     2221 ggaaattctg atatcaaaat aagtcgtccc ggttcatttt tcggcacatt ccggggtggc
     2281 ttcaatgcag aacaatatta cagcgtaact cttgacagaa attcctccct gaatggcgac
     2341 aactacatta ccaatctgta tttcataccc aattctaaca tccgaatcca ggcatttaca
     2401 acccagaatg ttaatttcat tagcgccaac ggtaatgaag atcaaattat ttccatcttt
     2461 tcgagccttg aaggcgacca ggcaaatttc agcgctcccc caacgagttc ctttactgtt
     2521 gattatgtgc acctcatgga taataatgca actggcgggg gattttttaa tgccaccaat
     2581 agttcagata agggtaatgt cacggggtgg aatggattat tgcagtggca ggaaataact
     2641 tttgatcctt taaatgacaa tgcatttggg aattcagttg cgctaaatgg atcttcaact
     2701 tcgagtaatg cggttactta ctcagttttt actggtgctg gctcagtgac cggctccagc
     2761 ttaaacctgg atagccaggg attggttgga atcaaagcct cccaatcagg taattctacg
     2821 tatggtcctg ccaaaacggt gtttcaatat atttatgcgt ataattctcc ctatgagctg
     2881 gggactttta aacaagcgaa ttttgttctg ggccagccgg atttcttcac tcaagacgtt
     2941 gtgaacagtt ccagcgtatt tcctggtgtc agcagtgcgg tggttagctc aaacaaacaa
     3001 ctggcaattg cccaaaccgg gaggggaagg gtggtgcttt acaatagtgt acccaattcc
     3061 agtggaactc caggggatgt aatcctgggt aaacctgact ttacttcaac taattccagc
     3121 acaactgaca gggatatgaa tttcagccgc tgggtagctt ttagccctga tggcagtaaa
     3181 ctagtggttt ctgatggaaa caggatctta atttggaact ccatccctgc agtcaattat
     3241 acacctgcag atgtggtggt ggggcaatcc gggttttcta caaatgatcc gggattatcc
     3301 gatacccaat ttaatggtgt ttggggaatt gacattagtt ctgacggtaa actctttgtc
     3361 gtagacgcta ataataaccg ggtactaatc tttaactcca ttccaactga aaacaatgca
     3421 gcagcggatg tggttatagg tgcagatgat ttctttacag attcaggtgg aacaagcagc
     3481 acccaacttt tgtttcctat tgatgttgct gtttcggaac aaggtcaatt atttatttca
     3541 gattttcaaa atgacagggt catggtttat aattccgtac ccaccaccaa cggagctgct
     3601 gcggacctcg tccttggtca gccggatttc aacaccgcgc aacaattaga tccatctatt
     3661 gataacttta acggcccttt aggagtttcc actttcagag atcaatatct tgctatttcc
     3721 gatttcaaca atcatcgggt aatgatatac gatctggaaa atcttactcc cgccaatatt
     3781 attctgggcc agcctgaatt gtttcttaat gaacccttta acggcggcat ttccggcaga
     3841 agcatcatca atccttatca ggtgcacttt gatcctgccg gtaatttgct tgtttccggc
     3901 gcttcaatgc acagggtgat gttcttcggg gctcccgaca tcaatccgcc aaccattcaa
     3961 aatcaaacga gcaatacgta ttcattgatg agcggggaat ccatttccgc aaatgttaca
     4021 gatgatcgag ccaaccttac ggtctataca gagatttcag gaatctctca atctacatca
     4081 agcacaattg acctggtgga taatgggtca acctttagtg taccaatatc cttcctggat
     4141 aattcaagcg aagcattcgg tattgagtat accctaatcg ccagtgacgg gtataatact
     4201 acgcgtgatg ggccctatta catttactta gaaagcaatc agaatgaccg attatctacg
     4261 attagctcga ctaatcttcc ggaaggtacg caggagagca attaccggat tatttctatt
     4321 cctttaattt tggataaccc ggccttgact gctgtttttg gcgatgaact tggaaattat
     4381 gatccaaaaa actggcggat gtttagctgg aatccatctg gagcaggttc ctattctgaa
     4441 ctcaatgacg gaagtcaatt gcagattgga acaggttatt ttcttttgat ccgctcacga
     4501 cccagcggaa ctgtaaactt cggaaatgga agtgttcccc aggtaaacag gagttctcca
     4561 tttacaaaat cactgtccac gggttggaat ctaattggca atccttatcc ttttaatatc
     4621 gcttgggcag atattttgac cacaagcggc ttagcaagtc tcgacggaaa aatttatact
     4681 tcaaatggct tccagaatat cactacactg gctgcttaca gtggagcatt tgtcatggcg
     4741 ccggcagcta ctactttggt attccccata agcagcggag gcgaaatgcc aaatccggga
     4801 aacgagggtc agagggtaca ggatcagaat gattggcagg tgcaattttc tctcagatcc
     4861 ggtgaatttc acaatgatta tagcggcata ggtatgcatg aagaagctga tttttcaggg
     4921 gattattttg atggatatac acccccacgg tttacagctt acctggaaat gaattcgaat
     4981 catcccgaat caaaatataa aatcgctaag gatatagtcc ctgtagaaaa taatcatgtc
     5041 tggacttaca acctcgaaac caatgttccc gatgataaag tcaaactaag ctgggattta
     5101 ggcggttttg atccgggtga aaagcagctc atcatgatgg atatatcaaa taataaaatc
     5161 attgacatga catcaaggtc tgaatttgag tatccctatg ttgataacaa gcagataaag
     5221 attttctatg gaaccgggga ttttatcgcc gaacagctaa gtccggagaa gccatactta
     5281 agcaattatc caaatccctt cattggtcag acaacaatat cgtttggact tccagggtct
     5341 gaaaatgatc aattcagagt aagatttgag gtatttgata taaccggaca aatggttaca
     5401 agtatcgctg aaggggtata tatgtctgga tatcatgagc taacgttgaa caaagctcaa
     5461 ttgaatattc agctaaaatc agggatttat ttactgaaaa tgttggtttc tggaaattcc
     5521 gccaactatt ccagttcttt acggctaatg attaagtaaa taataccctt tggcaaggat
     5581 tttcattaat tttgtattaa ataaaattgc ataagccatg gtcaaaagat tgttgatttt
     5641 cagtgcatca gtagcagtaa gcttaaatgc atggtcacaa gagattaagg gcaaaagtga
     5701 acccttgtat gtcaattttc agcccgccac ggtcatcgcg gcaattccgg taataacatg
     5761 gataacaccg attgatcaga ttaccacatt gaaaataaaa aagggtagcg taaaggtggg
     5821 tgtcaattca gacattaaac taaagaacgt aactctttac attaacaatc ttcctacagc
     5881 taatgaccgg gggttatccg tttcacagcc tcaggatgcc ggctacgatg aaatctttga
     5941 aaaggaggtt tctcttaatg acggcgataa cgaaataaaa attgtggcag aagatataac
     6001 aggtaaggta acggtagaat ccagaatttt acgcgttgag ttacctgcgc ttatggccag
     6061 aaatgattat gcactattat ttgcaaccga tgagtacgat acctggggag atttgataaa
     6121 tccggttaac gatgcaacta caatcggaaa agagttggag gaagcttatg gattcaaagt
     6181 cgaagtgatc cgcaatccta ccatgactgg agtgcttacc aaacttagag agtacgcgaa
     6241 gaaaagctac caggaaaccg atcagctttt tattttcatt gccgggcacg gtcaatttga
     6301 tccatttttc aatcaaggat atttagtatg caaggatagt agaaaggatg atgaagttaa
     6361 agcgacttat ttatcgcatt ccaacttaag agaaattgta aataacattc cttccaagca
     6421 cgtatttctt gcaattgacg catgctttgg tggaactttt gatcctgtaa ttgcaaaggc
     6481 aggtcataga ggcggtgatg atatgtacag tgaattatct tctaaagaat atattgaaag
     6541 aaaattaaga ttcaagaccc gtcgctacct gacttccggc ggaaaagaat atgttccgga
     6601 tggaagaccc ggaagacatt ctccctttgc ttcaaaaatg ctggaggcgc tccgtaatta
     6661 tggcggctcc gacaagatta ttactttacc tgagttgatg ggatttattg aaaaaattaa
     6721 ccctgaacca agaacgggtt catttggaga tgatgaacca ggaagtgatt tcgttttcgt
     6781 tgcaaaataa catgataaga aatattgttt tgggagctgc catgttggtg gcttctccaa
     6841 ttttagccca gcgccatggc actgggctaa gttttgatga caatgcttac caaaattctc
     6901 aggcaaaagc aatcctgaca cgtggtgact accaggcagt accgtcggca gcttctctga
     6961 aacaatattg tccgccggca ggtaatcaac ttcaactgaa taccagtgtg gggtgggcgg
     7021 tctcttatgg cgctatgact attctgaaag ccaaacaaaa taattggaca gataagcata
     7081 agattcagca aaatgctttt tcaccaattt attcttatta taaggtgaag ccggaaaatg
     7141 atattgattg ttctgcaccc attagtctgg ataaggcact gaattcgctt aagaatgaag
     7201 gagctccaaa atttgtagaa tttcttgagt tttgtcccaa tgacattgac aattctcaag
     7261 ttcctcaaaa gcaatccgta accggattta atagacttta tgatataaat gctgcttccg
     7321 attttaaaac atctgctgta aaaaaagcaa tagcggaagg ataccccgtc gtagtaggaa
     7381 tgtatgtgtc cccttcattc aacacggcta aagaattctg gcaacccagg gagaaatttg
     7441 attccaaatt tccagggctt gccctttgtg ttgtgggcta tgatgataat aaatatggcg
     7501 gagctttcga agttatgaat agttggagca gttcctgggg aaatgaaggt ttcatttgga
     7561 taccatacaa ttattttaat gaattttccc ggtatgcttt tgagatttat gatgttcccg
     7621 aaaaaaccaa tgcggatttg ggcggtgcgc tttcatttca gcttcctgat aaaagcatga
     7681 tgcccgtttc attgataaat aatgaagggg attataaaat ttccaaaaat tatggcctcg
     7741 gcactgtatt cagaataatc atcagtaata atgccacagc tttcgtttat gcgctcggta
     7801 ccgatatttc tggaaatgta tttcagattt tcccgatgga aggaatcagt cccgttctgc
     7861 cttacaaatc aaataatgtc cctttgccat ccgaagaaga tgttatcgaa gtcacagaag
     7921 cctccggcag ggattatgtc ggaattattt attcccggga agaactgcct atccaaaata
     7981 tagttagtca aatgaactca tcttcagaag cctttccggt tcgattaagg aaattattag
     8041 gtcccagact cgttaaaggt ggagaagtga actttggtga ttcagaaata aattttcagg
     8101 gaaaggcaac caatggttcg gcagtggtaa taaccgcgga gttagagtat aaatagttct
     8161 gctcactcag tcagatttta actgcatggt aatcgataag aagaaattcc tggttatttc
     8221 atttgtggca tctggcatct tatttattaa ttcaattttt gtggtccagg aatcatcttt
     8281 tgaagagagt attaagaggg gtgaaatcat ttacaaaaaa gactgcatta cctgtcatat
     8341 ggaaaatggc gaaggtattc ctggagtatt tcctcccctg gctaaatccg attttctgct
     8401 tgaacacctg gacaaaagta tctctgcaat tataaatggt ctgtcaggtg aaatttcagt
     8461 caacgggcaa atctactttg gtcaaatgga gcctgtcaaa ttaactgacc aggaaatagc
     8521 tgatgttctg aattattcaa gaaatagttg gggaaataaa gcaaatgaaa ttaaagcgga
     8581 agaagtagcg gcccaaagaa ctaaataatt atttcgctga cataaaagct tcaatattcg
     8641 ttaatatgga tcgaacttcc cggtccattt caatacgtac cttgctccat cccacatcat
     8701 caccttcaaa ctcgcaaagt ccatgagcct ttcccaagtt ttgacaacgc tgttgtgttt
     8761 tcaatgaaat gtgataaata tcatagacct tgtatctgag tacactggaa tttagattga
     8821 cagataatgt caggccaaat gaataattgc tatcgacctc ttcaattaaa gatatccgga
     8881 gaattttttt gagaaggata ccggatgaag aactctgagt aaagattaaa gtgtcctgtt
     8941 cattcgtaac ctgaccggaa gcccaggtcg agattctaaa gatagcactg tccagattct
     9001 gattcaaaac ttcgacgctg tcttcataaa aggcattacc ttcctcatcc aattgaaatt
     9061 caatatcttg tgcaggcgtt aagttgctgc atatcaaaat aaaaattatc cacccaaatt
     9121 tctgtctttt caacattcga acatttaacc ccaacataac taaaaaccgg atatttatag
     9181 aattagaacc tcaggaatta ataaattgaa ttccataaca cttaaaataa cgacaatgga
     9241 taagccttac tacaaaccgg aagatttggg aaaatttggt cagataaccc aatggcaaga
     9301 agagttgggg aaaaaattct ttgattatta taactcggtt ttcgaaccag gcgccttaac
     9361 agccagggag aaagcgttaa ttgcactggc agtcagccat acggtgcagt gtccttattg
     9421 tattgatgcg tacactaccg ccagtctgga aaatggtgcg gatgaaaaac agatgatgga
     9481 ggcggttcac gttgccgcgg caatccggag tggatcaagt cttgttcatg gggtacaaat
     9541 gatgaataaa tccagggaga tactgatgta aatggtgaaa ctcagcttcg cttattttag
     9601 atactcctta atacacattg atcccctcaa agaggacagt ttcaccaggt gagatcagat
     9661 ttccaatcaa agtcagcgta tcattgaatg agcctgatag caccattttc gtcttatgaa
     9721 ttgtggggta taacctgaaa gtaaacgcgc cctgattagt aaaaatagtt ccggtggcat
     9781 agatctcttt accggaagtt ttcacttcat aattctctac ctttcctctt agggtcatac
     9841 cacctatgcc attccatcct ctcataccag gcagcattag ttgaacgacg ccgtcttttt
     9901 gtttaactgc aacaaagtta ttcggagtat tgagaaaacc gtgattgttc ctttcatcca
     9961 ataacgccgt aacttccagt acccattcgg aattggtaac catttccttt attttggcct
    10021 caactgcctc ttgctcttgc agtctttgct cctttttttc ttgcctggtt agttttttat
    10081 ccgacctgat ttcctgagga aatgccacaa atgggataaa aactattatc actattaaga
    10141 atcgtctcat atcacctccg tttcctgatt aattgcaaaa agtattccat attgaggttt
    10201 ggcaagtttt gagattgagc aatgaaaatt ccatttataa acgcgtaaat aagtccgcgt
    10261 caaagcccct atgggactat ggcgggcgaa gtggagccgg agggattcga accctcgtcc
    10321 agataaggaa acctgcagct ttctacatgc ttatttttcc ttggttttcg agccgggctt
    10381 ggtggaaaac aaaccaaaac ccagccttag ttgcaattga gtttcacttt gacatcacaa
    10441 cacctgctaa agctagtttt gcatttcgat gtcctgtag
//
LOCUS       DASWHX010000087         2331 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_2660145, whole genome shotgun sequence.
ACCESSION   DASWHX010000087 DASWHX010000000
VERSION     DASWHX010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 2331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2331)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2331
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2660145"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..418)
                     /locus_tag="VGA21_05935"
     CDS             complement(<1..418)
                     /locus_tag="VGA21_05935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650078.1"
                     /translation="MNLQTWIRIQTFGSAIIKFEIVMVNIRFSFISLPVIVLLLWSCS
                     DDNPSVSIPVNLSNEWITGNLEEANIGNLDDAIMAAEEIPRFTSLLMVRDGKLVLEEY
                     FHGFHADSIHDVRSVTKSIVSTLTGIALDVGFISSID"
     gene            complement(648..992)
                     /locus_tag="VGA21_05940"
     CDS             complement(648..992)
                     /locus_tag="VGA21_05940"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014020068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEX9650079.1"
                     /translation="MKVKQFEIWIADLNPVMGTEAGKIRPVIVVQTDLLNKEHPSTIV
                     CPITTNVKPDSEILRVHLKKSKFRLKEDCDIMIDQVRAIDNKRLVKKVGVVDSDTADK
                     IRENLKIVLDLG"
     gene            1604..>2331
                     /locus_tag="VGA21_05945"
     CDS             1604..>2331
                     /locus_tag="VGA21_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009283667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HEX9650080.1"
                     /translation="MNLRIRTLLALFIFVFAFAGTAQIPRPSETFGFEPGADYKMAGY
                     DQMLAYYEKLAASTDRVQMIEIGKSVHGRPIKLLFISSEENMKSLDKWREISEKLARA
                     RIDESEATRLSKEGKAIIWFDGGMHATEKAHAQMTPELMFRIASEETDEMKRIRNEVV
                     TMIVPVINPDGLDLVDSWYKSNLGTPFETTNPPVLYQEYVGHDNNRDWFMSNMPETKA
                     ATTVLYNQWYPQIVHNHHQSSPAWA"
BASE COUNT          686 a          480 c          429 g          736 t
ORIGIN      
        1 catcaatgct ggaaatgaaa ccaacatcca gggcaatccc tgttaatgtc gaaacaatac
       61 tttttgtgac agagcggaca tcatgtatgc tatcagcatg aaatccatga aagtattctt
      121 ccaaaaccag cttaccatcc ctaaccatca gaagacttgt aaatctgggg atttcttcag
      181 cagccatgat ggcatcatcc agattaccaa tgtttgcttc ttccaggttt cctgtaatcc
      241 attcattgga aaggttaaca ggaatactta ccgagggatt atcatcagag caactccata
      301 gtaatagtac tattactggg agtgaaataa aggaaaacct gatatttacc atcacaattt
      361 caaatttaat aattgctgaa ccaaaagtct gaattcttat ccatgtttgg agattcacct
      421 atgaaatact aacattccgg ccaactttga ccattccgga tttattgccg gcagcgtatc
      481 acctgagcag tcagcgatgc gcatgtctaa agaattagcc ttacccttat aacacctggc
      541 tatttgcagt ggctggcttt tgaaactata ttgtccaccg taaccaaacg ttatagcgaa
      601 gataaaaatt ttcggcttac actttcccca gcaattgcaa atagctgtta gcccaggtcc
      661 aaaacaatct ttaaattctc tctaatcttg tcagccgtgt cgctgtccac aactccaact
      721 ttttttacaa gtcgtttgtt gtcaattgcc ctgacttggt caatcattat gtcacaatct
      781 tctttaagtc tgaactttga ttttttcaaa tgaactctca gtatctcgga gtccggcttt
      841 acgtttgtcg ttatcggaca gacaatagtc gatggatgct ccttatttaa taaatcggtc
      901 tgaacaacaa ttacaggtct gattttcccg gcttctgtcc ccattactgg atttaggtct
      961 gcaatccaga tttcaaattg tttaaccttc atccgttcag tttttcaaac tcagccaaga
     1021 tttccaatga atccttagaa acgatttttg attccttact tagctgcttt gaaagaagtt
     1081 tccgtttatg aaggcggttg taaaattgga tggcttcgtt tatatatcta ttccgactct
     1141 ttttgacctt tgtcacaatt ttctcagtct cctggaaaat gtcgtcctca agttttaatg
     1201 ataaattttt catagctata ccttttgcca aaggtatata ctttttagat atactccaaa
     1261 tttgtttgga tgtccgccgg ccatgaacgc gaaacaatgg tactactttc aaaaccttga
     1321 tatagaatta aagtattgga tagagtttta attgttcata atcgcctctc cctttgtgga
     1381 gttttttatt tgttgggttt gaaatctgat aaactgaatt aaagcattta catatctcgt
     1441 ttgctactga acttaaagat tattaattca ttatatttta ttcattatca atataataga
     1501 taaccctgct taaattaaaa ctcaaaattt ccatttgagt aaatgctatt ggccttatcc
     1561 tgtggattcg gtaatattga cctttaacaa aaaacctgca gttatgaacc tgagaattag
     1621 aaccctgctc gccctattta tttttgtatt tgcctttgcg ggtacagccc aaattccaag
     1681 accatccgaa acttttggat ttgagcctgg ggccgattat aaaatggccg gatatgatca
     1741 aatgcttgcc tattatgaaa agcttgcggc atcaactgac cgcgtgcaaa tgattgaaat
     1801 tggtaaatct gtgcatggtc gtccaattaa attgctattt atttcgagtg aagaaaacat
     1861 gaagtcattg gataaatggc gcgagataag cgagaagctt gccagagcca ggattgatga
     1921 atcggaagct accaggctct ccaaagaggg taaggccata atctggtttg atggcggcat
     1981 gcacgcaaca gaaaaagccc atgcgcaaat gacccccgaa ttaatgttta gaattgcctc
     2041 tgaggaaacc gatgaaatga aacgcattcg gaatgaagta gtaaccatga ttgttccggt
     2101 aataaatcct gatggtcttg atttggttga ttcctggtac aagagtaacc tgggaactcc
     2161 ttttgaaact accaatccac cggtgcttta ccaggaatat gtgggtcatg ataataaccg
     2221 ggattggttt atgagcaaca tgcctgagac aaaagcagcc acaaccgtat tgtataatca
     2281 atggtatcca caaattgtgc ataatcacca ccagtcttct ccggcgtggg c
//
LOCUS       DASWHX010000088        19607 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_2660406, whole genome shotgun sequence.
ACCESSION   DASWHX010000088 DASWHX010000000
VERSION     DASWHX010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 19607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19607
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2660406"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2445)
                     /locus_tag="VGA21_05950"
     CDS             complement(<1..2445)
                     /locus_tag="VGA21_05950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650081.1"
                     /translation="MKERKGNMIMLLRYLLIIAIAVFCIINTFIYPIRINTSIEKEIE
                     LSKADNPDEYYDFYDRIRTREGDARPQYQQGYKEIALKKALQTSQNLRLTTHEPPVWE
                     SLGPANVPGRTRAILVDVSDPTGNTWFAGTAGGGIWKTSTGGVGWINKTPEIPNLATN
                     SIVQAPSNPDVMYAGSGEGYGIAGSIKGDGIYKSIDHGDTWNLLTSTLNDYRFENINR
                     IIIDPDDENILLVCTNTHALDPQFFSGILKSLNGGTTWQLVYEGSSPVQQLIHHPSDF
                     NIQYATQFANTVLKTEDAGETWVPASTGLVSGGRIELAIAFSDPNRLYAATEGNTIME
                     DGSELFVSYDAGLSWSLLKEVNSGENFNWFGGQGFHNNSLVVNLLNENEIYVGGIDLH
                     KMKLEAGQAMEGNRLLNVFEINSVPFLGWINWGGGFLGGGFDTGEKWFSTGSWTGYPE
                     NFSPSDMVSAEIRFGNGLTQMAHRFIVDPISGSNNDGGAGVSPLGHHYADYVEVPFQV
                     WDIDNNIQLMVSFRDQERDGKFNLNERDDNDVDLLNVREYIFIHAISYDPANPNPNIT
                     KDGGHGYKNIYSMWPKLRRGSIWNPLNLPDSKVQAIYGPLMIGFKSTINLTSAKGILS
                     LNTVHPDHHNIIPILETASTFKLLDANDGGIYLSDLSTNPGVGDSKWVKASNQMVTGQ
                     FYGIDKKPSANIYIGGLQDNGVWLSGQNPNQSANYTLKIGGDGGEVVWNYRNPDKVIT
                     SNQYNRLFISTDAGANWSTVSGYPDKGVGNAPFITRIGNSKVAPDLIYLVGSSGVSRS
                     ADFGNSWTTTAINNQWS"
     gene            complement(2458..4431)
                     /locus_tag="VGA21_05955"
     CDS             complement(2458..4431)
                     /locus_tag="VGA21_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009582114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX9650082.1"
                     /translation="MLKPIPNGKSRCKFISTWTYHSTQLLFVFSLLWPGPILAQLSTK
                     SKKAEKLYYQAQSNITLRAYDLAIPLLKEAVEKDENFLEAQYMLGVSYKMMFRNDLAK
                     LPLEIANQLNKENKVPGANFHLGEIYFKEERYQETSELMERFLSSDPKGYNKDVAEEI
                     LANCKYAIEGIKKKISFNPAPLPESINRFQLQYFPALTVDQKTLFYTRRLGINPNDDE
                     DIVVSFKNSSGVWNAPFSISDNINTRYNEGTCTISADGRMLIFTSCFGRKGVGSCDLF
                     MSVREGNDWSEPINLGMPVNSKAWESQPSLSADGRTLYFVSDRGGGYGKRDIWMSTLN
                     DNNEWTTPVNLGPRINSAKDDISPFIHANGQTLYFASQGQLGFGGYDIYFTELTEDGW
                     SQPENIGYPINTSDDQVSLFITADGSKGYYSHEEIINGHRLEGKLYEFDIPPEIRVTH
                     RSNYVTGRVFDASTKKEIKAEIELFDLANQARISKVESDRMTGQYFIVLTEGSEYALY
                     VNKVGYLFQSLSFNYLEEVNLDPINIDIHLEPITEGIKTTLNNIFFEFDKYELKEKSI
                     TELNEINRFLSNNPTLKIEISGHTDNKGSDIYNNELSLKRAKAVYDYLLESGIERVRM
                     SYKGYGANEPIVSNETEEGQALNRRIEFKVITK"
     gene            complement(4367..4984)
                     /locus_tag="VGA21_05960"
     CDS             complement(4367..4984)
                     /locus_tag="VGA21_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020530737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HEX9650083.1"
                     /translation="MKRDSQLEVLPLMEAFYTIQGEGYHQGKPAYFIRLAGCDVGCPW
                     CDVKESWDAARHPKADIDKIVQEAGKSKAEIAVVTGGEPFMYNLQSLSHLLKKSGFRT
                     HIETSGAYPITGKWDWITFSPKKYKPPLAEFLDNCDELKIVVFNKTDFNWAEKYAQKT
                     HKKAKLYLQPEWSKARKVIPLIVDYVKTHPKWEISLQIHKYMDIP"
     gene            complement(4986..5885)
                     /locus_tag="VGA21_05965"
     CDS             complement(4986..5885)
                     /locus_tag="VGA21_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018476091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HEX9650084.1"
                     /translation="MSANQVDNLTNILDGRATANSILDELKLEVDKMKMEGKRPPHLA
                     VILVGGDGASRTYVKNKVAACEKVGFLSTHLQFENSVSEEDILGEIDKVNNDPEIDGL
                     IVQLPLPEQIDPSKVTQRIHPAKDVDGFTVENFGRIASKLPAYQPATPFGVLELLRRY
                     HIDTTGKHCVIVGYSRIVGAPLSMLLAGPGKATVTCCHIYTRNLSFHTLQADILIVAV
                     GKPGLITGDMVKEGAVVVDVGTTRVQDLTKKRGYALLGDVVFEEVAPKASYITPVPGG
                     VGPMTIASLLINTLHAASKNIYP"
     gene            complement(5888..7681)
                     /gene="lepA"
                     /locus_tag="VGA21_05970"
     CDS             complement(5888..7681)
                     /gene="lepA"
                     /locus_tag="VGA21_05970"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018476090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HEX9650085.1"
                     /translation="MGMDRIRNFCIIAHIDHGKSTLADRMLEHTHTVTMRQMQNQFLD
                     NMDLERERGITIKSHAVQMNYKYGGEEYILNLIDTPGHVDFSYEVSRSIAACEGALLV
                     VDASQGIEAQTISNLYLALEHDLEIIPVLNKIDLPGANPDEVIDEMVDLIGVRREDVI
                     LASAKEGIGIGEILDAIVVKIPPPDGDVNAPLQAMIFDSAFNSYRGVEVMFRVFNGSL
                     RKGDKVKFVNTGQTYEAEEIGIMKITKIPKETIAAGNVGYMISGIKVAKEVKVGDTIT
                     HVERPCKGAIKGFEDVKPMVFAGIYPVDTSEFEELRSSLEKLQLNDASLVWEPETSAA
                     LGFGFRCGFLGMLHMEIVQERLEREFDMTVITTVPSVRFYTIKSDGTKKEINAPSEMP
                     DPGSIHHVEEPFIKAQIITKSEFIGNIIALCMDKRGIIKNQVYLTQDRVELSFELPLS
                     ETVFDFFDKLKTISRGYASLDYEVIGYRESNMVKLDIMLNGEKVDALSAIVHRDKAYE
                     WGKKLCEKLKELMPRHMFEIAIQATIGTKVIARETVKAMRKNVLAKCYGGDITRKRKL
                     LEKQKKGKKRMRQVGNVEIPQEAFMAVLKIN"
     gene            7827..8492
                     /locus_tag="VGA21_05975"
     CDS             7827..8492
                     /locus_tag="VGA21_05975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650086.1"
                     /translation="MSFIKCIALLCVLSYSSSHTQAQKIENSPTIADILSGDFRVAFL
                     GIDLSETKFIGDKEDWPGAEFIKTLNPAWNNLLAYDLRKYRILPRALRLKPELVEYYP
                     NICTIHNDKLSFGEAIMPVSFGEEINNDKIQSILDSYDFTDLTGIGLMIFIESFDAKN
                     SLGTMYFTFLNLNTHKIIYSEKLNGNPAGVTPRKYWVETIHDVISQVQEIKYREWKKA
                     YLR"
     gene            complement(8511..9203)
                     /locus_tag="VGA21_05980"
     CDS             complement(8511..9203)
                     /locus_tag="VGA21_05980"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX9650087.1"
                     /translation="MMSSPDIIGDALKSFHYHKDSTPLIVNTNYGDAEEMLPQSLYRE
                     KDAIPDYEWVMVDQARGKTLDIGAGCGSHSLLLQEKVLEVVAVEISSGAAEIARLRGV
                     INIKNEDFKNYQHEKFDTITMVMNGIGVVGSLKGLTDSLQKFKTMLNPGGQLLFDTTD
                     ISYLGRKTTSSEYFGEVSFQFEYKGSKGAWFNWLYLDITTLRSVCNSHGWKMQIIYQD
                     EEDHFVMKLQPV"
     gene            complement(9207..9656)
                     /locus_tag="VGA21_05985"
     CDS             complement(9207..9656)
                     /locus_tag="VGA21_05985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4440 domain-containing protein"
                     /protein_id="HEX9650088.1"
                     /translation="MKKFAAFVLVIAGLTAVYYYFAREEENLDVSEIRSLLNRQTICW
                     NLGNIECFMMGYWESDSLIFIGRSGITYGYQNTLEGYKERYSDKSAMGELKFDIMEMR
                     KISDEVYLVVGKFYLERESLEDLEGIFTLTLKKIKDKWAIVADHTTG"
     gene            9720..10328
                     /locus_tag="VGA21_05990"
     CDS             9720..10328
                     /locus_tag="VGA21_05990"
                     /inference="COORDINATES: protein motif:HMM:NF016239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HEX9650089.1"
                     /translation="MNLLAHLHLSGDSDNIRIGNFIGDFVRGNPKGVFPPLVCKGILM
                     HRMIDEYTDQHEIVRASKSKLKGKYRHYSGVIVDIFYDHFLAVNWNTFSKQSLEEFTQ
                     ECYSLLNDHQEILPPKVNYMIKYMVKENWLFNYRHLEGIGRSLFGMSRRTKFDSKMDQ
                     AIKELELHYKDFQNEFLAFYPQLRQKADEFLEEVGGKKVGIR"
     gene            complement(10939..11535)
                     /locus_tag="VGA21_05995"
     CDS             complement(10939..11535)
                     /locus_tag="VGA21_05995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650090.1"
                     /translation="MKKILLFAALILAFSCRVENNSSNLVIQLQNYLEAKAAKDNSHW
                     QYMADTVYTWFDKKTGAGNIQLKGVPASGIWTEWDQEMNAYIKYDSLIPDDEIHSVSG
                     IFYEFNDFYSLIGKGPTKTKRTYWFDEENLIKEILIVWDPSNTMTAVHLKPVVEWVLR
                     NDSIEIQELYPNGEIIPSAENARKWKVLLDKYNKAADF"
     gene            complement(11678..14476)
                     /locus_tag="VGA21_06000"
     CDS             complement(11678..14476)
                     /locus_tag="VGA21_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009582123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEX9650091.1"
                     /translation="MKNLTCIIFSFIFLLSINAYCQNADIEAAVKSRSDQLANSLLSN
                     YPVRNVGPVEQGGRISDIEVVPGNPKHYYVGYASGGIFYTKNNGITFDPIFENQGFLG
                     IGDFALAPSNPQIIYVGTGEKNSSRSSYAGSGIYRSNDGGKTWSHLGLSNTQHIGRII
                     VHPTNPETVWVASLGALYTNNVDRGIYKSTDGGKTWHKTLFVNDSTGAIDLVLHPQNP
                     NQLLASTWERSRKAWDFKEGGPGSSIYRSEDGGNTWVKSAAGFPEGNGVGRIGLEISA
                     SNPDIVYALLDNQEETREEVVRPSDGSLQIQDFISMTRDQFQALDDKKLDAFIRSSGY
                     PQKYNARVVKREVRSGKYEPKALAEYFGDANAALFNTNVKGAEVYRSDDGGKTWSKAN
                     SYALDGVYFTYGYYFGEVRVDPKDSDKIYIFGVPLLKSLDGGKTYHRLDTMGRVHVDH
                     QALWINPDDPEHLLLGNDGGLYQSYDEGASWLHINNVSVGQFYSISVDMEKPYNVYGG
                     LQDNGVRKGSSTSIPNRTESWDGIFGGDGMFVYADPRNSNTVYAGSQFGFYMRLNLEP
                     DGRPSRITPRHDIGEPPLRYNWRTPIMISPHNADVIYMAAQKVYRSMNQGDDWESISG
                     DLTKNLPNGNVPYSTITSMSESKFKFGLVYIGTDDGNIQVTKNGGGSWELINNGLPVR
                     KWVSGVFASPHDEGTVFAALNGYREDDFKTYVYMSADYGKTWTSVKGNLPESVANVII
                     QDPVQPELLYTGLDIGSYVSFDKGKTWSLLSGIPNAPSYDMVVHPRDYELVVGTHGRS
                     VYVADAKPLQAVAEKGESTPVIAFEMESMRYFGRWGERRNEYTEPFTPSTSIMYYVGI
                     PANEITVTIKTEKGEVLREMKASGKKGFQKLSWDLKKTEYDKKGKATGNLSYIERGKY
                     TIELKNGTSTATTTFELR"
     gene            14526..14966
                     /locus_tag="VGA21_06005"
     CDS             14526..14966
                     /locus_tag="VGA21_06005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650092.1"
                     /translation="MIVCKPKPASLFSLGIFSIGGIAVGGFSFRIILTQINVPWYHYL
                     AALTLFPLAIVIFVRIFWTYKRVEFGREMVSVSYPFRFFLFSIPLKEIKTWKETRVKT
                     KTGNYREIDIISGSRKVSINLQEHSHYLEALKYLEKKAAKTKVR"
     gene            14963..16123
                     /locus_tag="VGA21_06010"
     CDS             14963..16123
                     /locus_tag="VGA21_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020526964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HEX9650093.1"
                     /translation="MKEFITNPLYIFSMIAALIIISEWLVKKTILKAGGTALLVIILM
                     AIVTNTGFAPPGTEAGNVYGVIQNYMAPLAIFYLLLDVNLKNLSKAGLPMLLMFFIGS
                     AGTFVGCLVAYFLVPEMKEFGEFSAPLSGMFAATYTGGGLNFNAVALHYGMMEQGNLF
                     AGAVAVDNVMTTLWIIVTIQGPLILQRFFPRTNKVKAETKSLDKEIIEDHIQFNSFAE
                     LIFWGILILLISNSLSNYIQSAFNFTIPSILILTTFSLVMAQIKRFHTMKGNQTLGLY
                     LVYFFLATVGAYCEIPTMIGLGSLAMTLFLFVLIAMVIHGLVIFGTGALLKMDWHLVA
                     IASQANIGGQSTAMALAKSFGKNDLILPAILVGTLGNGLGTYLGFLLAAMLG"
     gene            16196..16618
                     /locus_tag="VGA21_06015"
     CDS             16196..16618
                     /locus_tag="VGA21_06015"
                     /inference="COORDINATES: protein motif:HMM:NF016955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HEX9650094.1"
                     /translation="MFSFFRKSNKNAVLNESFNKLYGFTQDQKTAILVSLYEVANSDD
                     EFHPKETEYHRKIGGYLGLNYSARKLKDLLARDRDRIYKLLDNMNESQKDWYVITVLG
                     MIYSDGTIIQEELNHVISFLKGIGFSEERIRRNMVNEQ"
     gene            complement(17135..17749)
                     /locus_tag="VGA21_06020"
     CDS             complement(17135..17749)
                     /locus_tag="VGA21_06020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650095.1"
                     /translation="MEKKVPSAAVEYIVYDNLDRVALTQDGNLRAQNQWYFTKTDRLG
                     RPVYSGIYTNATQTISAGVQALLNALDYSADPYCEKPEVNATYHGYSNDAFPSSGTEL
                     LTVNYYDDYDFDRDGNADVSYDDNELTGQETNLLSSTRFRATGSKIKYLEDTIWMTSA
                     VFYDHKGRVIQSYSEHHEGGSDLTTVIYDWEGKRLKNQVRTDTR"
     gene            18386..19117
                     /locus_tag="VGA21_06025"
     CDS             18386..19117
                     /locus_tag="VGA21_06025"
                     /inference="COORDINATES: protein motif:HMM:NF012870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HEX9650096.1"
                     /translation="MADQDMEFLRQVVLRKKLIKTAKAFYLVFSAVAKLFQLAANKAE
                     LLKQHTALILQTIQRVKPTLGVKRVLRWLHLSPAKMYYWMEDKNCSLSLSALCRSKHP
                     AQLLDSEVKTIKHYLLDDRFKSWSSLSVYYQALREKAVFVGINTWYKYARRLGIKRKF
                     FRLKHKMQIGIRASRPLEILHSDVTLFKPLDNTRMYVYLLIDNFSRYILAWKASLEYS
                     SEIALSVLRDALRKTSCNPTRWWKQ"
     gene            19114..19371
                     /locus_tag="VGA21_06030"
     CDS             19114..19371
                     /locus_tag="VGA21_06030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650097.1"
                     /translation="MNKHLKYYYLFKKDLKDFNETFLYLEKSIEDYNHKPHGKLFGLT
                     PFDVLNGKMPARNLFRNQIKLASRLRIQQNMTLQCCNENQK"
BASE COUNT         5436 a         4266 c         3878 g         6027 t
ORIGIN      
        1 gctccactga ttattgatgg ctgttgtagt ccaggaattg ccgaagtcag cagatcttga
       61 aactcctgaa gaacctacca ggtaaattaa gtccggagca acttttgaat tgccgattcg
      121 agttataaat ggtgcattac caacaccttt atccggataa ccactgactg tactccagtt
      181 tgctccggca tcagttgaaa tgaatagtct gttatattgg ttagaggtta tgactttatc
      241 aggattcctg taattccaga ccacttcgcc tccatcacct ccaattttca gggtataatt
      301 agcagattga ttaggatttt ggcccgataa ccaaacgcca ttgtcctgaa gtcctccaat
      361 gtagatattg gccgagggtt ttttatcaat tccatagaat tgtccggtaa ccatttgatt
      421 gcttgcttta acccatttgg aatctccaac cccgggattg gttgataagt ctgataggta
      481 gattccgccg tcgttagcat ccagtaattt aaatgtgctt gctgtttcaa gaattggaat
      541 aatattgtga tgatccggat gcaccgtgtt gagacttaga ataccttttg cggatgttaa
      601 attgatagtg ctcttaaatc caatcattag cggaccataa atagcctgca ctttggaatc
      661 cggtagattg agaggattcc aaattgatcc tctgcgcaat ttcggccaca tagaatagat
      721 atttttatag ccatgtccgc catccttcgt aatattcgga tttggatttg caggatcata
      781 gcttattgca tgaatgaaaa tatactctct aacattcaac aagtcaacat cattatcatc
      841 cctttcatta agattgaatt taccatccct ttcctgatca cggaatgaca ccatcagttg
      901 gatattatta tcaatatccc atacctgaaa aggaacctcc acataatccg catagtgatg
      961 accaaggggt gaaacgccgg ctcccccatc attattagaa ccggaaattg gatctacaat
     1021 aaaacgatgt gccatttggg tcaggccatt gccgaaccgg atttctgcgg aaaccatatc
     1081 tgagggtgaa aaattttccg ggtagcccgt ccagcttccg gttgagaacc acttctcgcc
     1141 ggtatcaaat ccaccaccta gaaatcctcc gccccaatta atccatccca ggaaaggaac
     1201 tgaattaatt tcaaatacat taagcaatct atttccttcc attgcctggc cggcttccag
     1261 tttcatttta tgaagatcga taccccctac gtaaatttca ttttcattga ggagatttac
     1321 taccaggcta ttattgtgaa aaccctgacc gccaaaccaa ttaaagtttt cgccactgtt
     1381 tacttccttt aacaaagacc atgacagtcc ggcatcatag ctaacgaata attccgagcc
     1441 atcttccata attgtatttc cttctgttgc cgcgtataac cggtttggat cggaaaatgc
     1501 tatggcaagt tcaattcggc cgccagatac aagcccggtg cttgcgggta cccaggtttc
     1561 tcccgcatct tcagttttta agacagtatt tgcaaactga gtagcatatt ggatgttgaa
     1621 atccgatgga tgatgaataa gctgctgaac cggagaactg ccctcataaa ccaactgcca
     1681 ggtggtacca ccatttagtg actttagaat tccggaaaaa aattgaggat caagagcatg
     1741 agtgtttgtg caaaccagaa gaatattttc atcatcagga tcgataataa tcctattgat
     1801 gttttcgaat ctgtaatcgt tcagggtaga agtcaacaga ttccatgtgt caccatgatc
     1861 aatagattta taaataccat cacctttaat tgagccggcg ataccgtatc cttctcccga
     1921 tcccgcgtac attacatcag gatttgaggg tgcctgaaca atgctatttg tggcaagatt
     1981 tggaatttca ggggtcttat taatccagcc aactccgcca gttgatgttt tccagattcc
     2041 accccccgcg gtaccggcaa accatgtatt gccagtggga tcagatacat caaccaaaat
     2101 ggcgcgggtt ctgcctggta cgtttgcagg acccaaggat tcccaaaccg gaggttcatg
     2161 ggttgttaat cgcagatttt gacttgtttg aagagctttt ttcagggcaa tctctttata
     2221 tccctgttga tattgggggc gggcatcacc ttcccgggtt ctgatacggt cataaaagtc
     2281 gtaatactcg tcaggattgt ctgcttttga taattcgatt tctttttcaa ttgaagtgtt
     2341 aatcctgata ggatagataa atgtgttaat aatgcaaaaa accgcaatag caattatcag
     2401 cagatacctg agtagcatta tcatatttcc cttcctctcc ttcatcaaaa ttatctttta
     2461 ttttgtaatg accttaaatt caattcgacg gtttaatgcc tgaccttctt cggtctcatt
     2521 agatacaatc ggttcattag cgccataccc tttgtaagac atcctgactc gctctatacc
     2581 tgattccagc agataatcat atacagcttt tgcacgttta agtgaaagtt cattattata
     2641 tatgtcactc cccttattgt cggtgtgtcc acttatctca attttgagag taggattgtt
     2701 agacaagaag cggtttattt cattcagctc tgtaatagac ttttccttca attcatattt
     2761 atcaaattca aagaatatgt tattgagagt agtcttaatg ccttcggtga tgggctcaag
     2821 atgtatgtcg atatttattg ggtccaggtt cacttcctct aaataattaa aggataaact
     2881 ttgaaaaagg taaccaacct tatttacata taatgcgtat tcggaacctt cggttaagac
     2941 aataaaatac tgtcctgtca tccggtcgga ttcaactttc gaaatcctag cctgatttgc
     3001 caaatcaaaa agttcaattt cagctttaat ttcctttttg gttgaagcat caaagaccct
     3061 cccggttacg tagttacttc tgtgcgttac tcttatttca gggggaatgt caaactcgta
     3121 taatttacct tcgagtcgat ggccatttat gatttcctca tgggagtaat atccttttga
     3181 accgtccgcg gttataaaca aactaacctg atcatcactg gtattgattg gatagccaat
     3241 attctcgggc tgtgaccagc catcctcagt taattctgta aaatagatgt catagccacc
     3301 aaatcccaac tggccttgcg aggcaaagta aagagtttgc ccgttagcat gaataaaggg
     3361 ggagatatcg tctttagcgg aatttattct tgggcccaaa tttacaggtg ttgtccattc
     3421 gttgttatcg ttgagagtac tcatccagat atctcttttg ccatatccac ctcccctgtc
     3481 tgaaacaaaa taaagagttc tgccgtcggc agataatgaa ggttgagatt cccaggcttt
     3541 tgaatttacc ggcattccca ggttaatcgg ttcagaccaa tcgtttcctt ccctgacact
     3601 cataaacaaa tcacagctac ctactccttt tcttccaaaa caagacgtaa agataagcat
     3661 gcggccatca gcagaaattg tgcaggttcc ttcattataa cgggtattga tattatctga
     3721 aattgagaaa ggtgcattcc aaacacctga ggagttctta aatgaaacca caatatcttc
     3781 atcatcattc ggattaatac caagccttct ggtataaaaa agtgtctttt gatcaacagt
     3841 aagggccgga aaatactgca gttgaaatct atttatactt tctggaagtg gcgccggatt
     3901 gaaggagatt tttttcttaa ttccttctat tgcatatttg caatttgcaa gaatttcttc
     3961 tgccacatct ttattatatc ctttaggatc tgaagaaaga aacctttcca tcaattcgct
     4021 tgtttcctga tatcgttctt ccttaaaata aatttctccc aaatgaaaat tagcaccagg
     4081 aactttattt tccttattta gctggtttgc tatttccagg ggaagttttg ccaaatcatt
     4141 tctgaacatc attttgtaac tcactcctag catgtactgt gcttccaaaa agttttcatc
     4201 cttttcaaca gcttccttca acaaaggaat tgcaaggtca taagcccgca aagtgatatt
     4261 ggattgagcc tggtaatata gtttttccgc tttctttgat tttgtagaga gttgtgccag
     4321 aattgggcct ggccataata agctgaagac aaacaataac tgtgtactat ggtatgtcca
     4381 tgtacttatg aatttgcagc gagatttccc atttgggatg ggttttaaca taatcaacta
     4441 ttagaggaat tacttttcta gccttactcc attcgggttg aaggtacaat ttagcctttt
     4501 tatgggtttt ttgggcgtat ttttctgccc aattaaagtc agttttattg aaaacaacaa
     4561 tcttaagctc atcgcaattg tccaaaaatt ctgccaaagg gggtttatat ttcttaggag
     4621 aaaaggttat ccaatcccat ttcccggtaa tcgggtatgc acctgaggtt tctatatggg
     4681 ttcgaaatcc tgattttttc aagagatgag acaatgactg caggttgtac ataaaaggtt
     4741 cgccacctgt tactactgct atttcagcct ttgactttcc tgcttcttgt acaattttat
     4801 caatatcagc ctttggatgt cttgcagcat cccacgactc tttcacatca caccacggac
     4861 atccgacatc acatccggca agccggataa agtaagcagg cttcccctgg tgatatcctt
     4921 ccccctgaat agtgtaaaag gcttccatta atggaagtac ttctaattgg gaatcgcgtt
     4981 tcatgctaag gataaatatt ttttgatgcc gcgtgcagcg tgttgatgag cagtgatgcg
     5041 attgtcattg gacccactcc ccccggaacg ggtgtgatat agcttgcttt cggtgcaact
     5101 tcttcgaaca caacatcacc gagaagggca taacctcttt ttttggttag gtcctgcacg
     5161 cgggtggtcc caacatctac gacaactgca ccttctttca ccatatcacc tgtaattaat
     5221 ccgggtttac ctaccgctac tatcaagata tcggcttgaa gagtatggaa cgagagattt
     5281 ctcgtataaa tatgacagca tgttactgtt gcctttcccg ggccggccag cagcatgctt
     5341 aatggggcac caacaatacg gctatacccc acaataacgc aatgtttacc tgtagtatca
     5401 atatggtacc ttcgcagtag ttcaagaacg ccaaacggag tggcgggctg ataggcaggc
     5461 agcttggagg caatacggcc aaaattctca acggtaaaac catcgacatc tttagctgga
     5521 tgaattcttt gagtgacttt tgatggatca atttgttctg gaaggggaag ttgaacaatt
     5581 aacccatcta tttcagggtc attatttacc ttatctattt ctcccagaat atcttcttcg
     5641 gaaacggaat tttcaaattg caaatgggta gaaagaaagc ctactttttc acaggcagcc
     5701 actttgtttt tcacgtaggt tcgggacgca ccatcacctc ccaccagaat aacagcaaga
     5761 tgcgggggtc gcttcccttc cattttcatt ttgtcaacct ccagcttcaa ttcatcaaga
     5821 atggaattgg cagtagctct tccgtcgagt atgttagtta gattatccac ctggtttgct
     5881 gacatcatta attaattttt agcacggcca tgaatgcctc ctggggtatt tccacattac
     5941 ccacttgtcg cattcttttt ttgcctttct tttgcttttc caataatttt cgtttcctgg
     6001 tgatatcgcc tccataacat ttggccagaa cattttttcg catggctttt actgtctcac
     6061 gagcgataac ttttgtacca attgtcgctt gtatggcaat ctcaaacata tgccgtggca
     6121 tcaattcctt tagtttctca caaagtttct tgccccattc atacgcctta tccctgtgaa
     6181 cgattgctga caacgcatcc actttctccc cattcagcat aatatccaat tttaccatat
     6241 ttgattcacg gtatcctatc acttcataat ccagagacgc ataacccctg gatattgttt
     6301 tcaatttatc aaaaaaatca aaaacggttt cagaaagggg aagttcaaag ctcaattcta
     6361 ctctatcctg agtgagataa acctgatttt tgatgattcc ccgtttgtcc atgcaaaggg
     6421 caattatgtt tccgataaat tctgattttg tgatgatttg cgcttttatg aatggctctt
     6481 caacatgatg gattgagccc ggatcaggca tttcactggg tgcatttatt tctttctttg
     6541 ttccatctga ttttatcgta tagaaacgaa ccgatggcac cgtggtaatg actgtcatat
     6601 caaattcacg ctccaatctt tcctgaacaa tttccatgtg cagcattcca aggaatccgc
     6661 agcggaatcc aaatccaagt gcggcagagg tttcaggctc ccaaacgaga ctggcatcgt
     6721 tgagttgcaa tttctcgagg gatgaccgga gctcttcaaa ttcagaggta tccacaggat
     6781 aaatgcccgc gaataccatg ggttttacat cttcgaaacc tttgattgcg cccttgcaag
     6841 gcctttccac atgggtgatg gtatcaccaa ccttaacttc tttcgctacc ttgattccgg
     6901 aaatcatata cccgacatta cctgcggcaa tagtttcctt cggaatcttg gtaatcttca
     6961 tgattccaat ttcttctgcc tcataagtct ggcccgtgtt aacaaatttc actttatctc
     7021 cttttcgcaa ggagccgtta aaaacacgaa acataacttc cacgccccgg taggagttaa
     7081 atgccgaatc aaaaatcata gcttgcaatg gtgcattcac atccccatca ggaggaggta
     7141 tttttacgac tatagcatca aggatttccc caattccgat gccttctttt gcgctggcca
     7201 gaataacatc ttccctgcga acaccaatca gatcaaccat ttcatcaatt acctcgtcgg
     7261 ggtttgcacc gggaaggtcg attttattta aaaccggaat tatttccaga tcgtgttcaa
     7321 gtgcaagata aagatttgag attgtctgcg cttctattcc ctggcttgca tcaacaacga
     7381 gaagggcgcc ttcacaagcg gcaattgacc gggaaacttc atagctgaag tccacgtggc
     7441 cgggtgtatc gataaggtta aggatatatt cttcgccacc atatttgtaa ttcatctgta
     7501 cagcatgact tttaatggta attccgcgct cccgctcaag gtccatatta tccaggaact
     7561 ggttctgcat ttgccgcatt gtaacggtat gagtgtgctc cagcatcctg tcggccagcg
     7621 tgctttttcc gtggtcaatg tgtgctatta tgcaaaaatt tcttattcta tccattccca
     7681 ttcgggcatc ctcgtttaga agtgcaaaag tattgaatta gcgattaaca attggtatta
     7741 gcgtgcacat tgtaagcaaa aatgatttgg gcttggatta ttccgcatga tttttgagat
     7801 tgggaattga aacacaatca ggagtaatgt cgttcattaa atgcatagca ttattatgtg
     7861 ttctttccta ttccagctcg catacccagg cccaaaaaat agaaaatagt ccaacgattg
     7921 cagacatcct gagtggagat tttagagtag cttttttagg tattgattta tccgaaacca
     7981 aatttattgg tgataaggaa gattggccgg gggctgaatt catcaaaact cttaacccgg
     8041 cctggaacaa tttactggcg tatgatctgc ggaagtaccg cattttgccc cgagccctta
     8101 gactcaaacc cgaactcgtt gagtattatc caaatatctg taccattcat aacgataagt
     8161 taagttttgg tgaagcgata atgccagttt cattcggaga ggaaattaac aatgacaaaa
     8221 ttcaatccat attggattcc tatgacttta ctgatctcac ggggataggt ctgatgattt
     8281 tcatagaatc ttttgatgct aaaaatagcc ttggtacgat gtattttacc tttttgaatc
     8341 tgaatactca caagattatc tacagcgaaa agttaaacgg taatcctgcc ggtgttactc
     8401 ctcgtaaata ttgggttgaa acaattcatg atgttatttc acaggtgcag gagataaagt
     8461 acagggagtg gaagaaagcg tacctgaggt aatggttgtt tgttttttat tcaaacgggc
     8521 tgtagtttca ttacaaaatg atcttcttca tcctgataaa tgatttgcat cttccagccg
     8581 tgggagttac agacggatct caaagttgta atgtccaggt aaagccaatt gaaccacgca
     8641 cctttgcttc ctttatattc aaactgaaaa gatacttcgc caaagtattc actgctagta
     8701 gttttacgtc caaggtaaga aatatctgta gtatcaaaca aaagctggcc accaggatta
     8761 agcatggttt tgaatttttg cagggaatcg gtgagccctt tcaggctgcc taccactccg
     8821 atgccattca ttaccatagt gatggtatca aatttttcat gctgataatt tttaaagtct
     8881 tcattcttaa tattaattac tccccgcaag cgggcaattt cagcagcgcc tgatgaaatc
     8941 tcaacagcga caacttctaa aactttttcc tggagaagca gggaatgaga accacatccc
     9001 gcgcctatat ccaaagtttt cccccgtgcc tggtccacca taacccattc atagtcgggg
     9061 atggcatcct tttcacggta taatgactgc ggtaacattt cttccgcatc gccgtaattg
     9121 gtatttacga tcaggggcgt tgaatcctta tggtaatgga aagattttag cgcatcgcca
     9181 ataatatcag gagatgacat catcgtctag cctgtggtat ggtcggcaac tatagcccat
     9241 ttatccttta tctttttcag ggttagagtg aaaataccct caagatcttc caaagattct
     9301 ctttccaaat agaattttcc cactaccaaa taaacttcat cagaaatttt tcgcatttcc
     9361 attatatcaa acttcaattc acccatagcc gacttatcgg aatatctctc cttgtatcct
     9421 tcaagagtat tttgataccc gtaagtaatt cctgacctcc cgataaatat taaactgtca
     9481 ctttcccaat aacccatcat gaaacattca atatttccca ggttccagca gatagtctgg
     9541 cggttaagca acgatctgat ttctgataca tccaggtttt cttcttcccg ggcgaaataa
     9601 taatagacgg ctgtgagacc cgcaattaca agtacaaagg ctgcaaattt cttcatagaa
     9661 ccgttattaa gacataataa agctacatat aaatctgatt ttgcttaact ttattgaata
     9721 tgaatttact tgctcatttg catctttccg gagattccga taatatccgt atcggcaatt
     9781 tcatcggcga cttcgtgaga ggtaacccaa agggagtatt tccaccactg gtttgcaagg
     9841 ggattttaat gcaccggatg attgatgaat acaccgacca acatgaaatt gtgagagcca
     9901 gcaaatcgaa gttaaaagga aaataccgtc attactccgg agtgatagtg gatatttttt
     9961 acgaccattt tctggctgtg aactggaata ccttttccaa acaatcgctg gaagaattta
    10021 ctcaggagtg ctatagctta ttaaatgacc accaggaaat cctgccgccg aaagttaatt
    10081 acatgattaa atacatggtc aaagaaaact ggcttttcaa ttataggcat ctggaaggaa
    10141 ttgggaggtc attattcggg atgagccgca gaacgaaatt tgacagcaag atggatcagg
    10201 ctatcaagga attggaatta cactataagg attttcaaaa tgaatttctt gctttctatc
    10261 ctcagttaag gcaaaaggcc gatgagtttc tggaagaggt gggtgggaaa aaggtaggaa
    10321 taagatgagt gtaacggaga gtgctggtga caaacttttt tcctcccgct gataaccgcg
    10381 gaacttttct aaaaagaaat aaaactccag gttattttat cctgaccaat ttcagcgaaa
    10441 atctgcgcta tcttgccgga aaactttttt actcccgctg attccgctga taaccgcaga
    10501 acttttctaa aaagaaataa aactccaggt tattgtatcc tgaccaattt cagcgaaaat
    10561 ctgcgctatc ttgccggaaa acttttttac tcccgctgat tccgctgata accgcagaac
    10621 ttttctaaaa agaaataaaa ctccgggtta ttgtaccctg cccaattttc agcgaaaatc
    10681 cgcactatct tgccgggaaa acttttttcc tcccgctgat ctcgctgata accgcagaac
    10741 ttttccggag cattctgcac aaatccgcgc aatctgcggg agcttttttt taattccgct
    10801 gattccgctg ataaccgcag aacttttcca aaaaagaatt aaaactccgg ttattgtcac
    10861 atttcgattt aagatgaaac ctccccctga ctcttccttg aaaagtggtg ggaaattgtt
    10921 tgagatttga ttcaggattc agaagtcagc agctttattg tatttatcca gtaatacctt
    10981 ccacttccgg gcgttttcag ctgaaggaat gatttctcca tttggatata actcctgaat
    11041 ttcaatggag tcgtttctta atacccactc aaccacgggt ttcaaatgaa ctgctgtcat
    11101 tgtgttggaa ggatcccaga cgatcaggat ctccttaatc aaattttcct cgtcaaacca
    11161 ataggttcgc tttgttttgg taggtccctt tccaattagc gaataaaaat cgttaaactc
    11221 ataaaatatt ccggaaacag aatggatttc atcgtcaggg attaagctgt catattttat
    11281 ataagcattc atttcctgat cccattcggt ccaaattccg gatgccggca cccctttcaa
    11341 ctgaatgttg cccgctccgg ttttcttatc aaaccaagtg tacacagtgt cggccatata
    11401 ttgccagtga gaattatctt tagcggcttt tgcttccaaa taattctgca attgaatgac
    11461 caggtttgag ctattatttt caacgcggca agaaaatgct aaaatcaaag cagcgaaaag
    11521 caagattttt ttcatttgta aagataaaat tctatggtat cagattttgt taataaattt
    11581 taaataaaat gttgacctcc taaagcagtc cttcggaaag gattttgaaa aaaaagctgc
    11641 agtgtctttc agttctccta acataataag cagattctta tcttaattcg aaggttgtag
    11701 tagcggtcga ggttccgttt ttcaactcta tcgtatactt acccctttct atgtatgata
    11761 aattgccggt tgctttccct ttcttatcat attccgtttt cttcaaatcc cagctcaatt
    11821 tctgaaatcc ttttttacca cttgccttca tctctcttaa aacctcaccc ttttctgttt
    11881 ttatggtaac agttatttca tttgcaggaa ttcccacgta atacattatt gaagttgaag
    11941 gcgtgaaggg ttccgtatat tcatttctgc gctcacccca tcggccaaag tatcgcatgc
    12001 tttccatctc aaatgcaatt accggtgttg attcaccctt ttccgctacc gcttgtaacg
    12061 gctttgcatc cgccacgtaa acacttctgc catgagtacc taccaccagt tcgtagtccc
    12121 tggggtgcac taccatgtcg taggatggtg cattgggaat gcctgaaagc agtgaccagg
    12181 ttttaccttt atcaaagcta acataagaac ctatatctaa ccctgtgtat aataattcag
    12241 gctgcacggg atcctgaatg attacattcg ctaccgattc cggcagattt ccttttacag
    12301 aagtccaggt ttttccgtaa tccgcactca tgtacacata cgtcttgaaa tcatcttccc
    12361 ggtagccatt cagtgctgca aagacggtac cttcatcatg tggagatgca aatactccac
    12421 taacccattt ccgcacaggc aatccattat taatcagttc ccagcttccg ccaccatttt
    12481 tggtaacctg aatatttcca tcatccgttc caatgtaaac cagcccgaat ttaaattttg
    12541 attcagacat actggtaata gtagaataag gcacatttcc atttggcaga tttttagtta
    12601 aatctccact aatagattcc cagtcgtcac cctgattcat actgcgatag actttttgcg
    12661 cagccatata aataacatcc gcattgtgcg ggctgatcat gatgggtgtt ctccagttgt
    12721 agcgcaacgg tggctcgccg atatcatgcc gcggcgtaat tctggatggg cgcccgtcgg
    12781 gttctaaatt cagccgcata taaaatccaa actgtgagcc ggcgtaaacg gtattggaat
    12841 ttcggggatc cgcgtataca aacatgccat cacctccaaa aattccatcc caggattcag
    12901 ttctatttgg aatagaggtt gaagatcctt tccgaacgcc gttatcttgt aatccaccat
    12961 aaacattata gggcttttcc atgtcaacgc taatgctata gaattgtccg acagatacgt
    13021 tatttatatg cagccagctt gcaccttcat cataactttg atacaggcca ccgtcattac
    13081 ctaacagcaa atgctcggga tcatcggggt ttatccataa ggcctggtga tccacgtgaa
    13141 cccggcccat agtatccagc cggtgatagg ttttccctcc atccagtgat ttgagtaatg
    13201 ggacaccgaa aatgtaaatc ttgtcggagt ctttgggatc tacccttact tccccaaaat
    13261 aatacccgta agtaaaataa acgccatcaa gggcgtagga atttgctttg ctccaggttt
    13321 tacctccgtc atcggaacgg tagacttccg cacctttcac gttagtatta aacagggccg
    13381 cgtttgcgtc accaaaatat tctgccaatg ctttcggctc atatttaccg gacctcacct
    13441 cacgcttaac cactcttgca ttatattttt gaggataccc gctgctgcga ataaaagcat
    13501 caagtttctt gtcatcaagg gcctggaatt gatctctggt catgctaata aaatcctgaa
    13561 tttgaagact gccatccgaa ggtcttacta cttcttcacg agtttcctcc tggttatcca
    13621 ataacgcgta tacgatatca ggatttgacg cggaaatttc cagcccaatc ctgccaaccc
    13681 catttccttc cgggaatccc gcggcggatt ttacccatgt atttccaccg tcctctgatc
    13741 tgtaaatgga cgaacccggc ccgccttctt tgaaatccca ggcctttctg gaacgttccc
    13801 aggttgaagc caataattga tttggatttt gcggatgtag aaccaggtct atggcaccgg
    13861 tgctgtcatt gacaaataaa gttttgtgcc aggttttccc tccgtcagta gatttataaa
    13921 tcccacggtc aacattgtta gtatataatg cacccaatga ggcaacccaa acggtttccg
    13981 gatttgtcgg atgaactatt attcttccaa tgtgctgtgt attgctgagc ccaagatgac
    14041 tccatgtttt tcccccatca tttgagcggt aaattcccga gcccgcataa cttgacctgc
    14101 tgctgttttt ttcacccgtt ccaacataaa taatttgggg gtttgatggc gcaagggcaa
    14161 aatcaccgat acccaggaat ccctggttct caaaaatggg atcgaaagta atgccattat
    14221 ttttcgtgta gaagattccg ccggaagcat atccaacata gtaatgcttg gggtttccgg
    14281 gtacaacttc gatatcagag atccttccgc cttgctcaac cggaccaaca ttccgcaccg
    14341 gataattgct gagcagtgaa ttagccagct ggtcgcttct tgattttact gcggcttcga
    14401 tatcggcatt ctgacagtaa gcattaatgc tcagaaggaa aatgaaacta aaaattatgc
    14461 aagttagatt cttcatatag gttagattta gggctttaaa aataagcaat tatctttagc
    14521 gatcaatgat tgtttgcaaa ccaaaacccg cttcattatt ttctcttggg atcttttcca
    14581 taggcggtat tgccgtggga ggtttcagtt tccggattat tctaacccag atcaatgtcc
    14641 catggtacca ctacctggca gcactaacct tgttcccatt agccattgtt atctttgtca
    14701 ggattttctg gacttataag cgtgtggaat ttggaagaga aatggtatca gtaagttatc
    14761 cctttcgatt tttcttattc tccatcccat taaaagaaat aaaaacctgg aaggagacta
    14821 gggtaaaaac taaaacagga aactacaggg aaatagatat tatttccggc agcaggaagg
    14881 tgtccattaa cctgcaggag cattcgcatt accttgaggc gttgaaatat ttggaaaaga
    14941 aggccgccaa aactaaagtc cgatgaaaga attcatcact aatccacttt atattttttc
    15001 catgattgcc gccctgatta ttatttcaga atggctggtg aaaaagacga ttctcaaagc
    15061 cggtggaacc gcgttgctgg taataatttt gatggccatt gttactaaca ccggttttgc
    15121 gccaccaggt acagaagccg gcaatgttta tggagttatc caaaattaca tggcacctct
    15181 tgctattttt tatttgttgc ttgatgtaaa tcttaaaaac ctttcgaagg cggggttacc
    15241 catgctgctg atgtttttta ttggttctgc cggcactttc gttggctgcc ttgttgccta
    15301 ttttctggtt cctgaaatga aagagttcgg agagttctca gctccgctgt caggaatgtt
    15361 cgcggcaacc tatacgggtg gcggcttaaa ttttaatgcg gtggccttgc attacgggat
    15421 gatggaacaa ggaaatcttt ttgccggcgc agtcgctgtg gataatgtaa tgaccacctt
    15481 gtggatcatt gtgactattc agggtcccct gattttgcaa cgtttttttc caaggactaa
    15541 taaggtaaaa gcagaaacaa agagtttaga caaagaaatc atagaagatc atattcagtt
    15601 taattctttt gctgaactaa ttttttgggg aatacttatt cttttgatat ccaattctct
    15661 gagtaattat attcaatctg catttaattt tacgatacct tccattctta ttctcaccac
    15721 tttttcattg gttatggccc agataaaacg atttcataca atgaaaggaa atcaaaccct
    15781 cggcttgtac ctggtgtatt tctttctggc tacagttggc gcgtattgcg aaattccaac
    15841 tatgattggc ctggggtccc tggccatgac tttatttctt tttgtgttga ttgcaatggt
    15901 tattcatggg ctggtaattt ttggaacagg tgcactctta aaaatggatt ggcacctggt
    15961 tgccatcgcc tcacaggcaa atattggagg acaatccacg gcgatggctc ttgccaagag
    16021 cttcggcaag aatgatctca tccttcccgc tattttagtc ggcacgcttg gaaatggatt
    16081 gggcacctat ttgggattct tgttggcagc aatgttaggc taagactttt tgtctcatct
    16141 attgccattg gaatgaattt tcttaccttg taatttcatt aatcaacaat cggtcatgtt
    16201 ctccttcttc agaaaatcaa ataaaaatgc ggtgctaaat gaaagcttca ataaacttta
    16261 cgggtttact caagaccaga agacggcgat acttgttagc ctctatgaag ttgcaaattc
    16321 tgatgatgag ttccacccta aagaaacgga atatcataga aagataggcg gctacctggg
    16381 tcttaattac agtgccagga aattgaaaga tttactcgcg agagaccggg atcgaatcta
    16441 caaattgctg gataatatga atgaatccca aaaggactgg tatgttataa ccgttttagg
    16501 gatgatttat tctgatggta ctataattca ggaagaatta aatcatgtta ttagtttttt
    16561 gaaagggatc ggtttttctg aggagcggat ccgtaggaat atggtgaatg aacagtgagc
    16621 agtgaacagt catactttgc cgatagttaa aatttaagtg agagtaaaat ttcctcacca
    16681 tagtaatttt acttaatgca gggaaactct ttgaagtgtt catcggcatt tgtgcaggcc
    16741 aggaatttct tccttgatga aatcaagaaa gcttgacact taggcgccat tccgtttcat
    16801 tgatggtgct taaacaaatc agcattgtca gtaaatgtgc cttctccgca gtctgagcag
    16861 ttccatgcaa aacagctggc tggcgcttaa attaccgggg tcgttgattt gcttcagcca
    16921 gccccggatg ttgtaagtga ggttcacggt ttgcaaaaag ttttcgtcag ctttttcatg
    16981 cagtttcttt tgggttagtt gacccagttc gttgtactgg taagcgccaa ctattaccgg
    17041 ttcttcagaa tccagggtct gcgtaatttc cgtcagccgg ccgccatcat cgtagtcgta
    17101 ttccagaatt ttcgttatac ccgaaccctg caggttacct ggtgtctgta cggacttggt
    17161 ttttcagcct ttttccttcc cagtcatata tcacggtggt tagatcactt ccgccttcat
    17221 gatgttctga ataagattgg atgacccttc ccttgtgatc atagaaaact gccgaggtca
    17281 tccagattgt atcttcaaga tattttattt tacttccggt ggccctgaaa cgggtggaag
    17341 aaagaagatt ggtttcctgt ccggtcagtt cattatcatc ataactcaca tccgcgttcc
    17401 catcccggtc aaaatcgtaa tcatcgtaat aatttaccgt cagcaactcc gtaccggatg
    17461 agggaaaggc atcattgctg tagccatggt acgtggcgtt aacttccggt ttttcacagt
    17521 aggggtcggc ggagtaatcc aatgcattca gaagcgcctg gacgcctgca ctgatggttt
    17581 gtgtggcatt cgtataaata ccactataca ccggccggcc cagccggtcg gttttggtaa
    17641 aataccattg gttctgcgct ctcaggttgc cgtcctgggt cagggcaacg cggtccagat
    17701 tatcatacac gatatattcc accgccgcac tcggcacttt cttttccacc agccggcctt
    17761 tgctgtcgta ggtaaatttg aaaatcaggt ctttgaggct ggaggcggta tcatcaaacg
    17821 aagtggcggc accaatcgtt tctactgcct tgggcggcag ggtagccagc ggcctgcccg
    17881 ttgcattatt aagatttata gatgactaag tttggattgg attttggtaa taattttaat
    17941 atttttacat aacaacattc ccctttgtac agaataatga tgagacagtt gacccttgac
    18001 atagaggata gtaagtatag ggctttcctt gatttcataa aaacactcga ttatgtttcc
    18061 attaagcacg aaactataat accccagtgg caacaagagg aagtagaacg tcgtttgagt
    18121 ttagtggaaa gcgggaagat gaaaattcgg gactgggagg atgtcaaaaa gcatgtcttt
    18181 aaaaagaaat gacttactcc atttcaattt cggaagctgc cgaaaatgat atcagtgaag
    18241 cttatttatg gtacgaagat caaaaggaaa acttaggaag tattttcgaa gttcaatttc
    18301 agcgggctgt aaaaagcatt agaaacaatc ctttcaaaac acaaattagg tacggaaaca
    18361 tgccaaaata ctgggatgcg atgctgtggc tgatcaggat atggaatttc tcaggcaggt
    18421 tgtacttcgc aagaaattga tcaagacggc taaagcattt tatcttgtct tctctgccgt
    18481 cgctaaactc tttcaactcg cggctaacaa agcggaactc cttaaacaac atacggcttt
    18541 aattctgcaa accattcaaa gggttaaacc taccctggga gtgaaaagag tgctgcgttg
    18601 gttacatcta tctccagcaa aaatgtatta ttggatggaa gataaaaact gttcactatc
    18661 cctatctgcg ctttgccgtt ccaaacatcc tgctcaattg ctggattcgg aagtgaaaac
    18721 cataaagcat tacctgctgg acgatcgttt caaatcctgg agctcgctgt cagtttatta
    18781 tcaggccttg cgtgaaaaag cggtatttgt tggtataaat acttggtata agtatgccag
    18841 aaggttagga attaagcgga aattcttcag attgaaacat aaaatgcaaa ttggaatacg
    18901 ggcttccagg ccattggaga ttttgcattc cgatgtaacc ttgtttaaac ctttagataa
    18961 tacccggatg tatgtctatt tgctgattga taatttctcc cgctatattt tagcctggaa
    19021 ggcatccctg gagtattcca gtgaaatcgc cctgagtgtt ttgagggatg cgctcagaaa
    19081 gacatcatgc aatccaactc gatggtggaa gcagtgaata agcatttaaa atattactat
    19141 ctctttaaaa aagatttgaa agattttaat gaaacattcc tctacctgga aaaatccatt
    19201 gaagattaca accacaaacc gcacgggaaa ttgttcgggc taacaccttt tgatgtatta
    19261 aatggtaaaa tgccggcccg gaatctgttc cggaatcaga ttaaactggc atccaggcta
    19321 agaatacaac agaatatgac attacaatgc tgcaatgaaa accaaaagta gataggaagc
    19381 tgtcggctaa atagaattca ctctgcaacc ccaggaaaac ctgtccctga attcacagga
    19441 aaaacgcggt tcgaaatttc ggactccact tcaggtaatt agctggtgtt gtgctagcct
    19501 caatccctca cgcatgtgtt caatccaact cgatggtgga agtagtgaat aagcatttaa
    19561 aatattacta tctctttaaa aaagatttga aagattttaa tgaaaca
//
LOCUS       DASWHX010000089        17252 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_3035197, whole genome shotgun sequence.
ACCESSION   DASWHX010000089 DASWHX010000000
VERSION     DASWHX010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 17252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17252
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3035197"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(397..582)
                     /locus_tag="VGA21_06035"
     CDS             complement(397..582)
                     /locus_tag="VGA21_06035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650098.1"
                     /translation="MSASMAHVKGSTETLPERVNNIIIRKFQLSGVSTGYLQQKSVVE
                     VTVKWKWQLPRAAIARF"
     gene            824..1507
                     /locus_tag="VGA21_06040"
     CDS             824..1507
                     /locus_tag="VGA21_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009580682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEX9650099.1"
                     /translation="MAKTKTINGHPFIEYSRQTFSPEEAGKRSKEFFNFMDTRRTVRD
                     FSDQPIPRDLIDNLIRAASTAPSGAHKQPWTFCVVSDPAIKTKIRQAAEEEERESYAG
                     RMPDEWLKALEPLATNWEKPFLEIAPYLIIVFRKLYNLTESGAKENNYYVSESVGLAC
                     GFLLAAIHHAGLVALTHTPSPMGFLSKILNRPENEKPYLLIPVGFPAENTYVPDLKRK
                     ELGEVAIYF"
     gene            complement(1510..2991)
                     /locus_tag="VGA21_06045"
     CDS             complement(1510..2991)
                     /locus_tag="VGA21_06045"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009580676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX9650100.1"
                     /translation="MEKYDLAIIGAGPSGYAAAMRALDFKKKILLIERKRLGGTGVTN
                     GALSSKTWWEISTETSLVLRNLKRFNMDPPQINYKEIQGEVIKATDRRKDLLRSHMEL
                     LNGSVYKDLLNFELGNARLASPNEIEIETQNGKKTIWSDYVILATGSRPRELPHIPLD
                     EKIVMTSEGIEKLEDFPDSMVIVGAGVIGCEYATIFSGFGKTKVNLIDKGDRILPNED
                     DDVVKVIETNMEANGVLIHRNSRLTKMDIVDGKVNYELEYDDGRKEIFHVERALLSVG
                     RIPNYENLGLEEIGVNMDKSGIIDDNTQTSVPNIYAVGDITADIALVNVGELEGRYAV
                     ECIFGNPRRKLVYENISTIMFLNPEVAGVGLNEIQAKKKGIDYKVASLSYECIPRAIA
                     QRNTQGFIKLLVTNDEEMKILGMRVIGQHSSSAIQAVALLISMDKGIEELAECVHPHP
                     SIIEGIQEVVRMLMGKSLFKPDALKDKMRCSTYCNGVYEDILF"
     gene            complement(3044..3775)
                     /locus_tag="VGA21_06050"
     CDS             complement(3044..3775)
                     /locus_tag="VGA21_06050"
                     /EC_number="1.14.16.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008199442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine 4-monooxygenase"
                     /protein_id="HEX9650101.1"
                     /translation="MKQIYSNYTKENHLVWQILFDRQLQKLPNAACSEFLKGLERIAF
                     QPEKIPDFEITNSLLNILTGWKIQAVPGIVDDRIFFELLAAKRFPATTWIRRMDQLEY
                     LEEPDMFHDVFAHVPLLTNKDFCEFLQSLGKIALEHGHDAHAIHLISRIYWFTVEFGL
                     IDDTNGRRIYGAGILSSSGETDFSLSNKPVHLEYDVDQILDSSYRKDIFQSKYFIIKS
                     FEELFKSLSLIRKGLKVRLMEIMRK"
     gene            complement(3978..4559)
                     /locus_tag="VGA21_06055"
     CDS             complement(3978..4559)
                     /locus_tag="VGA21_06055"
                     /inference="COORDINATES: protein motif:HMM:TIGR04560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein THX"
                     /protein_id="HEX9650102.1"
                     /translation="MGKGDKKTRKGKISIGSFGVSRSRKKIKARKKKKSGRGIAKKEP
                     KITMVQPKKTPAKVEKAPAKVEEAPVEVVEVPEVVAKLEIRIPEPEVKEVKKESSITP
                     DKAKPKRVDAKPEKEKAKPKKEKAETKVKKEKAKPKKEKAETKVKKEAVKTKEKAKTK
                     VKKPAKKVVTKKAKTMAPGAKKTAKTTKKTTKK"
     gene            4703..5221
                     /locus_tag="VGA21_06060"
     CDS             4703..5221
                     /locus_tag="VGA21_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019989381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650103.1"
                     /translation="MVNPGIELLIALRKTISKLESGSAYQWGHMGCCNCGHLAQELTN
                     LTKAQIHEYAMQRTGDWNDQVLEYCPDSGLPIDAIIDSMLNAGLTIDYIRQLEKLSNP
                     GILAIIPSHDKPLSQNKRSDVIIYMNAWVKILEGNLMSEIKIHSLEDPWVQPIAKTRS
                     KFKYSPLVSSPL"
     gene            complement(5187..7808)
                     /gene="alaS"
                     /locus_tag="VGA21_06065"
     CDS             complement(5187..7808)
                     /gene="alaS"
                     /locus_tag="VGA21_06065"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015266831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEX9650104.1"
                     /translation="MKSWQIRRKFLDFFKSKGHQIVPSSPIVVKNDPTLLFTNAGMNQ
                     FKDYFLGNRNPDYKRVVDTQKCLRVSGKHNDLEEVGLDTYHHTMFEMLGNWSFGDYFK
                     KEAIEWAWELLTKEYGLNEDSLYVSVFGGDKEENLEPDQEAYDLWNQIIPKEKIIFGS
                     KKDNFWEMGDVGPCGPCSEIHIDLRPETEKKHTPGKELVNTGNPLVIEIWNLVFIQFN
                     RKSDGKLINLPQKHVDTGMGFERLAMAIQGKVSNYDTDIFTPIISEITKLAGIKYGEN
                     DSSDVAFRVISDHIRAICFTIADGQLPSNVGAGYVIRRILRRAVRYGNTFLGLKEPFL
                     YKLVKVIVNEMGDFFSELKDQKEFLEKVIMEEETSFLRTLENGLKRLASIKSTTKGKL
                     INGKLAFELYDTFGFPLDLTNLIAREDGYLVDETEFKAEMDKQKDRSKADAKKVTGDW
                     VELISDEPSGFVGYDNLTSDIKILKYRTVKEKEKNKVQAVFDQTPFYAESGGQVGDTG
                     RIQSENEMITILDTQKENNLIVHLLEKLPENPSSIFKAQVDGTRRKSIMNNHSATHLL
                     HAALREVLGDHVQQKGSLVADNILRFDFSHFSKMTNEELNKVEEIVNEKIQANIQRDV
                     KENMPIGEAKRMGAIALFGEKYGDIVRVVTFDPDYSIELCGGTHVPFTGQIGFFKITS
                     EGSVAAGVRRIEAVTGKGAINFVKGQVELINKLKEILKDPKDVVRSVRTLIEESSQLA
                     REVGALQRREQRDVKESLKKKLNTSGGINILIERIEVPSPEILKNIAFELRSEVKDIF
                     LVLCSVITDKPQIVVAISDSLVKSKSLNAASIVKELAKEIKGGGGGQPFIATAGGKDA
                     SGLNRVLTMGRKLAENI"
     gene            7973..8956
                     /locus_tag="VGA21_06070"
     CDS             7973..8956
                     /locus_tag="VGA21_06070"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017732215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX9650105.1"
                     /translation="MARIKYYYDTETCKYERVKVSTWDVLLNLLGFLTIATILAVGMV
                     VGYNAYFESPKEALLRKENDELKFHYDILQKEMDNVSQMLSALQERDDNIYRVIFEAD
                     PIPSTIRTAGVGGSERYKNLAEKDLQQRTLIINNFERVDKLKKGMYIQTKSYDEIIEL
                     ARNKSDMLAAIPAIQPIQNVELTRLASGFGMRIHPIYKARRMHWGIDFSAPRGTPIYA
                     TGDGVVDLAGTSLKKTGYGNQVEINHGYGYVTKYAHMQMLVVKKGEIIKRGQLIGYVG
                     SSGGSTAPHVHYEIMKDGKKIDPIQFIIQDVSDAEYQKLLELASRENQSLG"
     gene            8982..9956
                     /locus_tag="VGA21_06075"
     CDS             8982..9956
                     /locus_tag="VGA21_06075"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX9650106.1"
                     /translation="MSKRIFIYNPDTCDYEPYRPGRRKQMLKVTLFFLGALTLATSSL
                     WYLSDEFGTPKENSRTATRQDLINKIEMLDQDLDGFYNKLDQITERDESIYRIILEAE
                     PLSSGVRNAGSGGSEKFPEIVQTYLANKEKILIDYLKLDKLKRQLFIQSLSFDELVEL
                     AFERNRMWQSIPSIQPVDNKELRRLSTIYGMRFHPILKIVRPHYGLDFMAEIGTQIYA
                     TGNGVVKAAYYSDSFGRTVEIDHGYGYITRYAHMSEFLVKKGEQVKRGQAIGLVGNTG
                     LSVSAHLHYEVLKDYQNVNPIGFFQHELDEESYALLLELAKRETVPLD"
     gene            10210..11289
                     /locus_tag="VGA21_06080"
     CDS             10210..11289
                     /locus_tag="VGA21_06080"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013455028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEX9650107.1"
                     /translation="MPSVSITELPEIDTIPKIKPTDSLLVATGEITADSISIIGVGDI
                     MMGTNFPGPEFLPTDSGRYLLSSVAEILQSADVTFGNLEGVLLDEGGELKNCNNPKAC
                     YLFRSPLYLASNLAAAGFDLLSVANNHAGDFGNTGRQSTARCLDSLQILYAGFVDKPF
                     VLLELDKVTIGFTAFSPNTGTLSIHDKEKTREIVSHLDSISDIVIVSVHSGAEGAAYQ
                     HVTRENEIFFGEDRGNIYEFAHNVIDWGADVVFGHGPHVSRGIEVYKERFITYSLGNF
                     CTYARFNLRGPNGFAPIMKILTDGQGRFISGNIIPVKQIELGYPVLDEDLNAITIIRE
                     LSKKDFPESPLIIGDSGNILYIQNQ"
     gene            11294..11632
                     /locus_tag="VGA21_06085"
     CDS             11294..11632
                     /locus_tag="VGA21_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009577610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX9650108.1"
                     /translation="MPYKEKQIEKKYYTIGEVASMFNVATSLIRFWETEFDIIDPKKN
                     KKGNRQFTKEDIEDLKLIYHLVKERGFTLQGAKDFLKNDYQAAKDRMELIDSLSKVKV
                     FLQEVRSQLG"
     gene            11636..12736
                     /gene="dprA"
                     /locus_tag="VGA21_06090"
     CDS             11636..12736
                     /gene="dprA"
                     /locus_tag="VGA21_06090"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HEX9650109.1"
                     /translation="MREESFYQISLTCIPGIGSQTIKQLLSYCGSAKQVFTGPFGKLK
                     KIPGIGIKTIELLKQTKYFESAEKKLRLLENEGVKITFYNDDSFPKRLRHCIDSPVIL
                     LHKGVADYNSERILSVVGTRNASEYGKIVTNQIINELKKYDAVIVSGLAYGIDIAAHK
                     AALREGLVTWAVIAGGHNKIYPGSHTKVCHEIMNSGCVISEYLPDDIPEATNFPERNR
                     IIAGISDATLVIEAKETGGALITADIADSYDKEVFATPGPVGSHTSAGCHNLVKNNKA
                     HLVTSADDMAKLLGWDQLESTKPQLVKSDFSENEFSIISLLQEKEMHIDELSWKSCIP
                     LSSLANLLLNLEIQGIIKGLPGKKFKFIYSGK"
     gene            complement(12737..13585)
                     /locus_tag="VGA21_06095"
     CDS             complement(12737..13585)
                     /locus_tag="VGA21_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002700101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HEX9650110.1"
                     /translation="MKIIECPRDAMQGIEVFIPTAKKIKYLNQLLKVGFDTLDFGSFV
                     SPKAIPQMKDTPEVVDNLELQGSETKLLAIVANNRGAEEAIKYGAISYLGFPLSISET
                     FQKRNTNKTIVEALNEVEEIQNNCLKKGKTLVVYISMGFGNPYGDPYGTGIVAQFVGI
                     LNQLDIKIISLADTIGVSNTENISYLFSTLSKEYPHIEFGAHLHSRPDQSIEKIEAAY
                     NSGCRRFDGAIKGFGGCPMAEDSLVGNIATEAIINFFREKQIKLQLNLEQFKESVRLA
                     TQVFPV"
     gene            complement(13588..14544)
                     /locus_tag="VGA21_06100"
     CDS             complement(13588..14544)
                     /locus_tag="VGA21_06100"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018476269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HEX9650111.1"
                     /translation="MKVIEHLESVKGKTLFSIEILPPLKGDGIDGLFKHIDPLLEFKP
                     PFIDVTYHREEYAYRSVGNGLLERKTIWKRPGTVGICAAIQNRYHVDTVPHIICGGFT
                     VGETENALIDLNFLGIDNVLVLRGDAIKSEPRFIPEGNGHNYAVDLLCQVVNMNKGLY
                     LDQELANATPTNFCIGVAGYPEKHFEAPNPKVDMKYLKMKVDSGADYIVTQMFFDNQK
                     YFQFVKDCKEAGINVPIIPGLKPITSKKQVNTLPSLFHMDIPPELSDEIDKCKDNKTA
                     LEAGVEWCVQQSRELMEFGVPCLHYYSMGKAEPVLRIAKELF"
     gene            complement(14549..>17252)
                     /gene="metH"
                     /locus_tag="VGA21_06105"
     CDS             complement(14549..>17252)
                     /gene="metH"
                     /locus_tag="VGA21_06105"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020600211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HEX9650112.1"
                     /translation="NLSQEKQETRRFIRMASMHLSGLDPLIITPETNFVNIGERTNVT
                     GSRKFLNLIKEEKFEAALEIARDQVEGGAQILDVNMDEGMIDGKVAMVKFLNLIASEP
                     DIARIPIMIDSSKWEIIEAGLKCLQGKGIVNSISLKNGEKEFIAIARKVRMYGAAVVV
                     MAFDEAGQADTLERRIEICGRVYKILTEQLHFPPHDIIFDPNIFPVATGMEEHRRSAV
                     DFFQATKWIKENLPGSYVSGGVSNISFSFRGNNVVREAINSAFLYHAIEHGMDMGIVN
                     PTMLQVYDEIPRELLEKVEDVLLNTREDATERLLEFAETVKGKVNKSERDLSWRNDTV
                     ENRIKYALVNGIIEFIEPDVEESRNQMDKPLLVIEGPLMAGMDVVGDLFGAGKMFLPQ
                     VVKSARVMKKAVAYLVPYLEKEKDGKSKAKAKILMATVKGDVHDIGKNIVSVVLACNN
                     YEIIDLGVMVPPDKIISTAISEKVDIVGLSGLITPSLDEMVTVADEMEKAGMVIPLLI
                     GGATTSRIHTAVKIDPKYHGAVVHVLDASRSVPVAGELISDQRKAEAIKRIKSEYKGL
                     RENHERKNVEKTLISLPDSRANRFSAEYKSHITKPKSPGIQRFENYSLKEISEYIDWT
                     PFFQTWMLKGKFPFILEDKKVGVEAKKLYADAQEMLKEIIADKSLIANAAAGIFKANS
                     EGDDLIIFNEDGSVRTKFHFLRQQGKKAKDLPNLCLSDFVAPLNSGIDDYLGGFAVTA
                     GLGIEPLIEKYEKDHDDYKSIMIKSLADRLAEAFAELMHKKVRKEIWGYCPDENFSVE
                     ELIAEKYRGIRPASGYPACPDHTEKKLLFDLLAVEDSTGIYLTESFAMYPAASVCGLY
                     FAHPESKYFGLGKIQKDQIIDYSKRKHMNVGEAEKWLASNLSYL"
BASE COUNT         4937 a         3771 c         3178 g         5366 t
ORIGIN      
        1 ttacttttgt ctctgcaatc ataccgactt tttggagctt tcgaagtctt gaactggcta
       61 atggatctct tccaaagttt ggaattcacc tcatagtagg aaccttatgg tacctgatag
      121 ttttctattt gttgcacaga aggctgaaga atttttcgaa ctaggtcaga atctgcagaa
      181 cggaatcttt atttcctcct atcagaaaat tcccgcgttc gtttcttgat tatggcagaa
      241 tcaccagcaa aattttgccc atgatttaaa gatgaaatga ttgaacaaat actcatgctg
      301 attcttttat ttggaaagca taggctttaa aacaaaaata gccggaagaa ttttccagct
      361 tatgagatat ctctcttttt tgtgcgacaa tccgctctaa aatctggcta tcgccgcccg
      421 tggcaattgc cacttccatt tgacagtaac ttctactact gatttctgtt gcaaatatcc
      481 ggtagaaact ccggaaagtt gaaatttcct tattatgata ttgttaactc tttccggaag
      541 cgtctcggtt gagcctttta cgtgtgccat acttgcagac attgaaaatc aattaatttt
      601 cacccaaaaa aaagtggaag tgtggcatac gttgatgtta acttcatttg gatttactga
      661 gacactactc ccggcacatc aaccaaaagt ttcatactta gttctctgga tgctttattt
      721 tcttcataaa tattaagaat tatcagaata ctgaatcaca ggctaaatct ataattccag
      781 ttgcaaatcc acttatattt gtacagctca caaatctgac caaatggcga aaacaaaaac
      841 aataaacggt catcctttca tcgaatattc aaggcaaaca ttttcaccgg aagaagccgg
      901 taaacgaagc aaggaatttt ttaactttat ggatacacgc agaacggtta gagatttttc
      961 tgaccagccc attcccaggg atttaattga taatctcatc cgggcagcat ctacagctcc
     1021 ctcaggggca cataaacaac cctggacttt ttgcgtagtt tccgatcctg ctattaagac
     1081 taaaatcagg caggcggctg aagaagagga aagagaaagc tatgccgggc ggatgccgga
     1141 tgaatggctt aaggccctgg agcctttggc aacaaactgg gagaaaccct ttctggaaat
     1201 tgcgccatac ctgattattg ttttcagaaa actttataac ctgacagaat ccggtgcaaa
     1261 ggaaaacaat tactacgtaa gtgaaagtgt aggccttgca tgtgggttcc ttcttgcagc
     1321 catccatcat gcaggattgg ttgcactaac acatactcca agtccaatgg gattcctgtc
     1381 gaaaatttta aaccggccag agaatgaaaa accgtatctc cttataccgg ttggtttccc
     1441 tgcagaaaat acgtatgtgc ccgatcttaa gagaaaagaa ttaggtgaag tagcgattta
     1501 cttttaaggc tagaataaga tgtcttcata caccccgtta caataggtag agcaccgcat
     1561 cttatctttc agggcatcag gtttaaacaa tgacttgccc attagcattc gaacaacctc
     1621 ctggattccc tcaattatag aagggtgcgg atgtacgcac tcggcaagtt cttcaatgcc
     1681 tttgtccata cttatcaata aagccacagc ctgtatagca ctggatgagt gctgaccaat
     1741 aactctcatc ccaagaattt tcatctcttc atcattggtc accagcaatt tgatgaaacc
     1801 ctgggtgttt cgctgggcaa ttgcccgtgg gatacattca taacttaaac tggcgacctt
     1861 ataatcaata ccttttttct tcgcctggat ttcattcaac cctactcctg caacttcagg
     1921 attcagaaac atgatagtgg agatattctc atagaccagt tttcgcctgg ggtttccaaa
     1981 aatgcattca accgcataac gcccttccag ctcgccaaca tttaccaggg ctatatcggc
     2041 agtaatatct cctaccgcgt agatattggg cacactggtt tgggtgttat catcgatgat
     2101 ccctgactta tccatattaa caccaatctc ctccagtcca agattttcat aattgggaat
     2161 acggcctact gataacaaag ctctctccac atgaaaaatt tctttcctgc catcatcata
     2221 ttccaattcg tagtttacct ttccatcaac aatatccatc tttgttagcc ggctatttct
     2281 gtgaatgaga acaccgttag cttccatatt tgtctcaatc acttttacca catcatcatc
     2341 ctcattaggc aggattctat cccccttatc tatcagattt acttttgttt ttccgaaacc
     2401 ggaaaaaatt gtagcgtact cgcaaccaat taccccggct ccaactatca ccatactgtc
     2461 agggaagtct tctaattttt caatcccttc actggtcatt acaatttttt catccagggg
     2521 aatatgaggc aactcccgtg gtctgcttcc ggtagccaga ataacataat cagaccaaat
     2581 ggtcttcttg ccattttggg tttcaatttc tatttcattc ggagaggcta accttgcatt
     2641 tcctagttca aaatttaaaa gatctttata aacagaacca ttcagcagtt ccatgtgact
     2701 gcgcagaaga tcctttcgcc tatcagtggc cttaatgact tccccctgaa tttctttata
     2761 atttatttgc ggaggatcca tattaaacct tttaagattc cggagaacaa gtgaggtttc
     2821 ggtggatatt tcccaccagg ttttacttga taacgcgcca ttagttacac ctgttccgcc
     2881 cagacgcttt ctttcaatca aaagaatttt cttcttgaag tcaagggccc gcattgcagc
     2941 tgcataaccg gatggacccg ctccaattat agctaaatca tatttttcca tgggtcagat
     3001 taattcaaaa atcacgtcaa tatatagaaa atatgggaat tatctatttc cgcattattt
     3061 ccattaatct cacttttaac cctttcctta ttaaggacag ggacttaaat aattcttcaa
     3121 atgacttgat aatgaaatac tttgattgaa aaatatcctt tctataactg ctatcaagga
     3181 tctgatctac atcatactca agatgaacag gcttattact taatgaaaaa tcagtttcac
     3241 cactggatga taatattccg gcaccataga ttctccttcc atttgtatca tcgataagac
     3301 caaattcaac tgtgaaccaa taaattcttg aaattaaatg aatagcgtgt gcatcatggc
     3361 cgtgctctaa agctatttta cccagtgatt gcaggaattc acaaaagtct ttgttagtta
     3421 acagtggtac atgagcaaat acatcatgaa acatatcagg ttcctcaaga tactccaatt
     3481 gatccatcct tcttatccac gtggttgccg ggaatctttt ggcagccagc aattcaaaaa
     3541 agatacggtc atctactatg cccggtaccg cctgaatttt ccaacccgtc aaaatattca
     3601 aaagcgaatt agttatttca aaatccggga tcttttcagg ctgaaatgct atccgctcaa
     3661 gccccttcag aaattcggag cacgccgcat ttggtagttt ctgaagctga cgatcgaaca
     3721 gtatttgcca aaccaggtga ttctcttttg tgtagttact gtaaatctgt ttcatagtta
     3781 ttaggtttat agtgaaataa aaaacccggc acctttcgga accgggtcta actgtcttta
     3841 gtttcttatt tttttaatca gatagaactc acctattccg aaagatctta gatctgtcgt
     3901 aataagagta ataatatcta actatttgtt tcatcggtag caaaattaaa caatttacca
     3961 aattgtcaaa atcaatttca cttcttagtt gtcttcttcg tagtcttcgc tgtcttttta
     4021 gcccccggag ccatcgtttt tgcctttttg gtcacaactt tctttgcagg tttctttact
     4081 ttagtcttcg ctttttcctt agtttttact gcctccttct tcaccttggt ttcagctttc
     4141 tccttctttg gtttagcttt ctccttcttc accttggttt cagctttctc ctttttcggt
     4201 ttagctttct ctttctccgg tttagcatct actctttttg gcttcgcctt atctggagtt
     4261 atggatgatt cttttttaac ttccttaacc tcaggttcag gtatcctgat ttcaagtttt
     4321 gctaccactt ccggtacttc aactacttcg accggtgcct cttctacttt tgcaggcgcc
     4381 ttctctactt ttgccggagt ctttttcggc tgaaccatag tgattttagg ttctttctta
     4441 gcaataccgc gaccagactt ctttttcttc ctggccttaa ttttctttct ggatcttgaa
     4501 actccgaatg aacctattga aattttccct ttccgggttt ttttgtcgcc ttttcccata
     4561 tcattttatt gtaaaacagg ggcctaagat aatagtttat gattgaagtt tcataatcct
     4621 gtatcaaatt aaaggacatg taagggaaag gagcattttt catttttgta catttaaaaa
     4681 taatttttgg aaacggagat ttatggtcaa cccgggaatt gagttattaa ttgcgctgcg
     4741 caaaacaata tcgaaactgg aatcgggctc cgcgtatcag tggggccata tgggatgctg
     4801 taattgtgga catctggctc aggaacttac gaatctcacc aaggcacaaa ttcatgaata
     4861 tgccatgcaa cgaacgggtg attggaatga tcaggtgttg gaatattgtc ccgactccgg
     4921 gttacctata gatgcgataa ttgattctat gctcaatgcc ggactcacta ttgattacat
     4981 ccggcaattg gagaaacttt ctaacccggg aatacttgcc atcattcctt cccatgataa
     5041 acctttatcg cagaataaaa gaagtgatgt aattatatat atgaacgcct gggtgaaaat
     5101 tcttgaaggc aacttaatga gcgaaatcaa aattcattcg ctggaagatc catgggttca
     5161 accaatcgcc aaaacaagaa gtaaatttaa atattctccg ctagtttcct ccccattgta
     5221 aggactctgt tcaggccgga agcatcctta cctccggcag ttgcaataaa aggttgtcca
     5281 cctccgccac ctttgatttc cttagcaagt tctttcacga tggaagcggc attcagagat
     5341 tttgatttca cgagcgaatc tgaaatggct acgactattt gtggcttgtc cgtaataact
     5401 gaacagagta ccaggaaaat atctttaact tcacttcgaa gttcgaaggc tatgtttttt
     5461 aatatctccg gtgatggaac ctcaattctt tcaataagga tatttatacc tccgctggta
     5521 tttaatttct ttttcagaga ttctttcaca tctctctgct ccctccgttg caatgctcca
     5581 acctccctgg ctaattggct gctttcttca ataagagttc ttaccgatcg aacgacatct
     5641 ttgggatctt tcaatatttc ctttagttta ttgatcagct caacctggcc cttaacaaaa
     5701 ttaattgccc cttttccggt tactgcttct attcgtcgaa ctcccgcggc aacagaacct
     5761 tccgaagtaa ttttgaaaaa cccaatttgg ccggtaaaag gaacatgggt gccgccacat
     5821 aattcaatgg aataatcagg atcgaaagtt acaaccctga caatatcccc atacttttct
     5881 ccaaacagag caattgcacc catccttttt gcttccccga taggcatgtt ttccttaaca
     5941 tctctttgga tattggcctg aatcttttca ttaactattt cttcaacctt attgagttcc
     6001 tcgttagtca tttttgagaa atgagaaaaa tcaaatctaa gaatattgtc agccaccaac
     6061 gatccttttt gctgaacatg atctccaagt acttctctca atgcggcatg aagcaaatga
     6121 gtggcggagt gattattcat tatacttttt ctcctggttc catccacctg tgccttgaaa
     6181 atgctggacg gattttcagg tagtttttcc agcagatgta caattaaatt gttttccttt
     6241 tgtgtatcca ggatcgttat catttcattt tcagattgaa tccttccagt gtctccaacc
     6301 tggccgccgc tctctgcata gaacggtgtt tgatcaaaca cagcctgcac cttattcttt
     6361 tccttttctt taacagttct gtattttaaa atctttatat cgctggtcag gttgtcatac
     6421 ccaacgaaac ctgaaggctc atcactaatt agctcgaccc agtcgccggt tacttttttt
     6481 gcatccgctt tcgatcgatc tttttgctta tccatttcag ccttaaactc tgtttcatca
     6541 accaaatagc cgtcttctct ggcaatcaaa tttgttaaat caagaggaaa gccaaacgtg
     6601 tcatagagct caaaagctaa tttaccattt attaactttc ctttggtagt tgatttaatt
     6661 gaagcaagtc ttttcaaacc attttcaaga gttcttaaaa acgaagtttc ctcctccata
     6721 attactttct caagaaactc cttttgatct ttcaattcgg agaaaaaatc acccatctca
     6781 tttacaatca ctttcaccag cttataaaga aaaggttcct ttaatccaag aaatgtattc
     6841 ccgtagcgga cggctcgtcg aagtatcctt cggataacgt agccggctcc cacattggaa
     6901 ggcagctggc catcggcgat tgtgaaacag attgcgcgga tatgatcaga tatcacccgg
     6961 aaagcaacat cactgctatc attctcacca tatttgatgc ccgcgagttt ggtaatttca
     7021 gaaattattg gagtaaaaat atctgtatcg taattggaaa cctttccctg gatggccata
     7081 gcaagtcttt caaaacccat tcccgtatct acatgttttt gcggaaggtt tattaatttt
     7141 ccgtctgact tcctgttaaa ctgtatgaag accagattcc agatttcaat aaccagggga
     7201 ttgccggtat tcacaagttc ctttcccggt gtatgtttct tttctgtttc tggccggaga
     7261 tcaatgtgaa tctcagagca aggcccgcac ggaccaacat cacccatttc ccaaaaatta
     7321 tccttcttgc tgccaaaaat tattttttct tttggaataa tttgattcca aagatcatat
     7381 gcctcctgat caggttccag gttctcttcc ttatcgccgc caaacacaga aacgtatagg
     7441 ctgtcttcgt tcaaaccata ctcttttgta agtaattccc aggcccattc gatagcttcc
     7501 tttttaaagt agtcaccaaa agaccagtta cctaacattt caaacattgt gtggtggtaa
     7561 gtatccaaac caacttcttc aagatcatta tgcttacccg acactcgaag acatttttgt
     7621 gtgtccacca ctctcttata atcaggattt ctattgccaa gaaaatagtc tttgaattga
     7681 ttcatcccgg cattggtaaa cagcaatgtt gggtcgtttt taaccactat tggagaggaa
     7741 gggactatct gatggccttt tgatttaaaa aaatcgagaa attttcttct aatttgccag
     7801 gatttcatat aactttttca agctgagtgc ggccttaatt ggccctgagt taaaatatta
     7861 ttgctatatt gcagcctgtt tgtagagttt taaaccattc ctttaacaag ggagctctgc
     7921 aacaccttta atttggggtc aaaattatag taatttaccg tactgaggta aaatggcaag
     7981 aataaaatat tactacgata ctgagacttg taaatacgaa cgggtaaaag tttctacctg
     8041 ggatgtcttg ttaaatttgt taggctttct gacaatcgcg actatccttg cagtggggat
     8101 ggttgtcggt tataatgcat actttgaatc acccaaagaa gcgctcttga ggaaagaaaa
     8161 tgacgagttg aaatttcatt atgatattct tcaaaaggaa atggataatg tttcccaaat
     8221 gctttccgcg cttcaggaaa gagatgacaa tatttataga gtaatttttg aagctgatcc
     8281 gattccgagc acgataagaa cagccggagt gggtggttca gaaaggtata agaatcttgc
     8341 tgaaaaagat ttgcaacaaa ggacactcat tatcaataat ttcgaacgtg ttgataagtt
     8401 gaagaagggt atgtatatcc aaaccaaatc ttatgatgaa attattgagt tagcaaggaa
     8461 caagagtgat atgcttgcgg ctatacctgc aattcaaccc attcaaaatg tggagctaac
     8521 ccggcttgct tccggatttg gtatgcgtat ccatcctatt tacaaagccc ggcgaatgca
     8581 ctggggaatt gattttagtg ctccccgtgg aactcccatt tatgctacag gagatggtgt
     8641 agttgattta gccggtacca gtctgaagaa aaccggttat ggaaaccagg ttgagattaa
     8701 tcatggttat gggtatgtta ctaaatacgc ccatatgcaa atgctcgtgg tgaagaaggg
     8761 tgaaattatt aagagaggtc aacttattgg ttacgtggga agttcaggag gttcaaccgc
     8821 tccgcatgtt cattacgaga ttatgaaaga cggaaagaaa attgatccca ttcagtttat
     8881 tatacaagat gtttccgatg ctgagtatca aaaattgtta gaattggcat cacgggaaaa
     8941 tcaatcactc ggataacctc atcctaaata tcaaccaccc gatgtcaaaa agaatcttca
     9001 tctacaaccc ggatacctgc gactacgaac cttacagacc tggcagaaga aagcagatgc
     9061 tgaaagtcac tttgtttttt ttaggcgctc ttaccctggc cacaagttct ttgtggtacc
     9121 tgagcgatga atttggcacg ccgaaagaga acagtcgaac ggctaccaga caagatctca
     9181 taaacaagat tgaaatgctt gatcaggacc tggatgggtt ctataataaa ttggatcaaa
     9241 taacagaaag agatgaaagc atctaccgga taatcctgga agcagaacct ctatcatccg
     9301 gcgtaaggaa tgcaggaagt ggtggtagtg aaaaatttcc tgaaatagta caaacctatt
     9361 tggccaacaa ggaaaaaata ttaattgatt acctcaaact ggataaactt aagcggcaat
     9421 tatttattca aagtctttct tttgatgaac ttgttgaact tgcctttgaa cgcaacagaa
     9481 tgtggcaatc aattccaagt atccagccgg tagataacaa agaacttcga aggctttcta
     9541 caatttacgg aatgcgattc catcctattc tgaaaatcgt aagaccccat tatggcctgg
     9601 actttatggc agaaataggg actcaaatct acgccaccgg caatggggtt gtaaaagcag
     9661 cttactattc tgattcgttt ggccgaaccg ttgaaataga ccacggatat ggatacatca
     9721 cccgttacgc tcacatgagt gaatttcttg tcaagaaagg agagcaggta aagcgaggtc
     9781 aggccattgg gctggttggc aatacagggc tttcggtatc cgcgcactta cattatgaag
     9841 tgctgaagga ttaccagaat gtgaatccta ttggcttctt ccagcatgaa cttgacgaag
     9901 aatcctatgc cttgttactg gagcttgcca aacgcgaaac cgtcccgctt gattaatcaa
     9961 accttcactt taagattgtg tggaaaagta gttgataatt agtgtaaaaa aaattaagca
    10021 ctattaagct aaccttctat ttttgtagct tccaaatttt tatcaaccat ctacatcaac
    10081 cgcgtgaagg ggatatcgtt ttgtttcttt attgcgataa ttctgggatt ctattcctgc
    10141 aagacagcaa gaaggcctgt ttcaagcctt gatactgaaa aaggtaaact cgattctgct
    10201 caaattaaaa tgccttctgt ttcaataacg gaactacccg aaattgatac catcccgaaa
    10261 ataaaaccga cagacagctt gctggttgca accggggaaa ttacagctga ttcgatttca
    10321 atcattgggg ttggagatat tatgatggga acaaatttcc ccggtccgga atttcttccc
    10381 acggatagcg gcaggtattt gctcagcagt gtcgctgaaa tcctgcaatc ggcagatgtt
    10441 acctttggaa accttgaggg agtactgctt gacgagggcg gcgaactaaa aaattgcaat
    10501 aacccgaagg cttgttattt gttcaggtcg cctttatatc ttgcttccaa tcttgctgct
    10561 gcaggatttg acttactgag cgttgcaaat aatcatgcag gtgattttgg aaacacgggc
    10621 aggcaaagca cagccaggtg cctggatagc ctgcagattt tgtatgccgg ttttgtcgat
    10681 aaaccttttg tcttgctgga attggataaa gtcaccattg gctttactgc attttcacct
    10741 aataccggta cgttaagtat ccacgataag gaaaagaccc gggaaattgt ctctcatctt
    10801 gattcaattt ctgacattgt aattgtttct gttcattcag gcgctgaagg tgccgcatat
    10861 cagcacgtta ccagggaaaa tgagatattt ttcggcgaag acagaggaaa catctatgaa
    10921 tttgctcata acgttatcga ttggggagcc gatgttgtat tcggacatgg gccgcatgtg
    10981 tcacggggga ttgaagtata caaggaaaga ttcattacgt atagcctggg taatttttgt
    11041 acttatgcaa ggtttaattt acgcggccca aatggatttg caccaataat gaaaatttta
    11101 accgacgggc agggtagatt tatttccggg aatattattc cagtgaaaca aattgaatta
    11161 ggatatcctg tactggatga agatttgaat gcaattacaa taataagaga actttcaaaa
    11221 aaagattttc ccgagtctcc attgattatt ggtgattctg gtaatattct gtatattcaa
    11281 aaccaataaa cctatgcctt ataaagaaaa gcaaattgag aaaaaatatt ataccattgg
    11341 ggaagtagcc tcgatgttta atgtagcaac atctctaatt aggttttggg aaacagagtt
    11401 tgatataatt gatcccaaga aaaacaaaaa aggcaacagg caatttacaa aagaagatat
    11461 tgaagacctg aaactcattt accacctggt aaaagaacga ggatttacac tgcaaggagc
    11521 aaaggatttt cttaagaatg attaccaggc cgcgaaagac cgcatggaac tcatcgattc
    11581 cctctccaaa gtaaaagtat ttttgcagga agttcgttcc cagttgggat gatgaatgag
    11641 agaggagtct ttttaccaaa tatcccttac ttgtattccc ggcatcggaa gccaaaccat
    11701 taagcaactc ttatcctact gcggaagtgc taaacaggta tttacaggtc cttttggcaa
    11761 actcaaaaaa attcccggta ttggaataaa aaccattgag ttattaaagc aaactaaata
    11821 ctttgagtct gcagaaaaaa agctgcgact tcttgaaaat gagggggtta aaatcacttt
    11881 ttataatgat gatagcttcc ctaaaaggct cagacattgc atagactcac ctgttatcct
    11941 ccttcacaag ggagttgctg attacaatag cgaaagaata ctttccgtcg taggaacacg
    12001 taatgcatca gagtatggaa aaatagttac caatcaaata attaatgaac ttaaaaagta
    12061 tgatgcggta attgtaagcg ggcttgctta tggtattgat attgccgccc ataaggccgc
    12121 cctcagggaa ggcctggtca cctgggctgt aattgcaggt ggccataaca agatataccc
    12181 aggttcccac actaaagtct gccacgaaat aatgaattca gggtgtgtaa tttctgaata
    12241 cctccctgat gacatccccg aagcaacaaa ctttccggaa agaaacagaa ttattgccgg
    12301 gatatcagac gccactctgg tgattgaagc caaggaaacg ggtggagcat tgattaccgc
    12361 ggacattgcc gacagttatg ataaagaagt ttttgctact cccggacctg tcggcagcca
    12421 cacatccgca gggtgtcata atctcgtaaa aaataacaaa gcacatctgg taacatccgc
    12481 tgatgacatg gcaaaattat taggctggga ccagttggag tcaacaaagc cgcaattagt
    12541 aaaatccgat ttttctgaga atgaattctc aattatctcg cttttacaag aaaaggaaat
    12601 gcatattgat gaattaagct ggaaatcatg tattccattg agcagcctgg cgaacttgct
    12661 cctgaatctt gagatacaag gcattataaa gggtttgccc ggaaagaaat tcaaatttat
    12721 ttattccgga aagtaattaa accgggaata cttgtgtcgc caggcgaact gattctttaa
    12781 attgttccag attaagctgg agtttgattt gcttttcccg gaaaaagttt atgatcgctt
    12841 ccgtagcaat gtttcccacg agactatctt ctgccatagg gcagccgccg aatcccttga
    12901 tcgctccgtc aaaacgacgg caacccgaat tataggctgc ttcaattttt tcaatgcttt
    12961 gatctggcct ggagtgcagg tgagctccaa attctatgtg tggatattct ttcgaaagag
    13021 tagaaaacaa ataggatata ttttccgtgt ttgaaacacc gatggtatct gcaagcgaaa
    13081 taattttaat atcaagctgg ttgagtatcc ccacgaattg ggcaactatt ccggtgccat
    13141 aaggatcacc gtaaggattt ccgaatccca ttgagatata aacaaccaac gtctttcctt
    13201 tttttaggca attgttctga atttcttcaa cctcattcaa agcttcaaca atggtcttat
    13261 tggtattcct tttttgaaaa gtttctgaaa tggataacgg aaatcctaaa taacttatcg
    13321 ctccatattt aatcgcttcc tcagcaccgc ggttatttgc aacaatggcc aatagtttgg
    13381 tttctgagcc ttgcagttcg agattatcta ctacctccgg tgtgtctttc atctgcggta
    13441 tagctttggg agaaacaaaa cttccaaaat ccagggtatc aaatcccact ttcagcaatt
    13501 ggttcaggta tttgattttc tttgcagtgg gtataaatac ttctataccc tgcatggcat
    13561 cccggggaca ttcaataatt ttcataatca gaacaattct tttgcaattc gtaaaaccgg
    13621 ctccgcctta cccattgaat aataatgtaa acatggaacc ccgaattcca tcaattccct
    13681 ggattgctga acacaccatt ccacacctgc ctccagtgcc gtcttattat ccttacattt
    13741 atcaatttca tctgataatt ccggcggtat gtccatatga aacaaacttg gcaaagtatt
    13801 aacctgcttc ttcgatgtaa ttggcttaag gcctggaatg atcggaacat ttatcccggc
    13861 ttctttgcag tcttttacaa actgaaaata tttttggtta tcgaaaaaca tctgggttac
    13921 aatgtaatca gcgccactgt caactttcat tttcaaatac ttcatatcca cctttggatt
    13981 cggggcttca aaatgctttt ccggatatcc tgccactcct atacaaaaat tggttggtgt
    14041 agcgttagct aattcctgat cgagataaag tcctttattc atattaacaa cctggcagag
    14101 taaatctacc gcatagttgt gtccatttcc ttcgggaata aaccggggct cagacttaat
    14161 ggcatctcct cgcaacacca ggacattatc gattcccaga aagttcaaat ctatcagggc
    14221 attctccgtt tctccaacgg taaatcctcc gcaaataata tgaggcaccg tatctacatg
    14281 gtacctgttt tggatagcag cacaaattcc caccgtacct ggtctcttcc agattgtttt
    14341 cctttcaagc aaaccgttac caacacttct atacgcatat tcctcccggt ggtaggttac
    14401 atctataaag ggaggcttaa actccaaaag tggatctata tgcttgaaaa gtccatcaat
    14461 gccatccccc tttaacggcg gcagaatctc aatactaaat aaggttttac cttttacaga
    14521 ttcaaggtgt tcaattactt tcatgatctt ataaataaga taaatttgag gcaagccatt
    14581 tttcggcttc accaacattc atatgtttac gtttcgaata atcaatgatc tgatctttct
    14641 gaattttacc cagcccgaaa tacttcgact ccggatgagc aaaatataaa ccacatacag
    14701 atgcagccgg atacatggca aatgattcgg ttaaataaat tcctgtgctg tcttccacag
    14761 ccagaagatc aaatagtaat ttcttctcgg tatggtccgg acaagccggg tatccggatg
    14821 ccggcctgat tcctctgtac ttttcagcaa tcaattcttc cacagaaaag ttctcatcag
    14881 gacaatatcc ccaaatttct ttacgcactt ttttatgcat caattccgca aaggcttctg
    14941 caagtctgtc agccaatgat tttatcatta tgcttttata atcatcatga tctttctcgt
    15001 acttctcaat taatggttca atgccaagac cggccgtaac ggcaaatcct cccaggtaat
    15061 catcaattcc tgaattaaga ggcgccacaa aatcggaaag gcatagatta ggtaaatctt
    15121 ttgctttttt gccttgctgc cggagaaaat gaaatttggt ccgtaccgaa ccgtcctcgt
    15181 taaagattat caaatcatct ccttcagaat tggctttaaa aattccagct gccgcattgg
    15241 caattagcga tttatcagca ataatttctt tgagcatttc ctgagcatcc gcgtaaagtt
    15301 tttttgcttc cactcccact ttcttatctt ccaaaataaa aggaaatttt ccttttagca
    15361 tccaggtttg aaaaaaggga gtccagtcaa tatattcact aatttctttc agagaataat
    15421 tctcaaagcg ttgaataccg ggagattttg gttttgtaat atgggattta tactcagcac
    15481 taaatctatt tgccctggag tcaggcaaac ttatcagagt tttttcaaca ttttttcttt
    15541 cgtgattttc tctcagacct ttatattctg atttaatcct ctttatggct tcagctttcc
    15601 tttgatctga aataagttca ccggctacag gtacgcttcg ggaagcatca agcacatgga
    15661 caacagcacc atggtacttc ggatcaattt ttactgcggt gtgaatcctt gaagtagtgg
    15721 cacccccaat taagagcgga attaccatcc cggctttctc catttcatct gccaccgtaa
    15781 ccatttcgtc cagcgaaggg gttatcaatc cgcttaaccc tacaatatcc accttttcac
    15841 ttatggcagt acttatgatt ttgtccggag gcaccataac cccaagatct attatttcat
    15901 aattattaca ggctaacact acgctgacaa tattttttcc gatatcgtgc acatctcctt
    15961 tgacagtggc cattaatatt tttgccttgg ctttactctt cccatctttt tccttttcca
    16021 ggtacggtac cagataagcc accgccttct tcattactct tgcacttttt actacttgcg
    16081 gcaaaaacat tttgcctgct ccaaataaat ctccaactac atccattccg gccattaacg
    16141 gaccttcaat taccaacagc ggcttgtcca tttggttcct ggattcctca acatccggtt
    16201 caataaattc aataatacca tttacaagag catatttgat cctgttttca acggtgtcat
    16261 ttctccatga aaggtctctt tcggacttat taactttccc tttaactgtt tccgcaaatt
    16321 ccagcagcct ttcggtggca tcttccctgg tgttaagcaa aacatcttca actttctcca
    16381 gtaattccct gggtatttca tcatatacct ggagcatggt tggatttaca atccccatgt
    16441 ccattccgtg ctctatagca tggtagagaa aagcagaatt tatagcttct ctgacaacat
    16501 tattgccgcg gaaactgaaa gatatattgc ttactccacc actcacatag gagcccggca
    16561 agttttcctt aatccatttt gttgcctgga aaaaatcaac cgcgctcctc ctgtgttctt
    16621 ccataccggt tgcaaccgga aaaatgttgg gatcgaaaat tatatcatgc gggggaaaat
    16681 gtaactgctc cgttaatatc ttatagaccc ttccgcatat ttcaattctt ctttccagtg
    16741 tatctgcttg tccggcttca tcaaaagcca ttaccactac cgcagctcca tacattctaa
    16801 ccttcctggc aatagcaata aattcttttt caccattttt caggctgatt gaatttacaa
    16861 tgccttttcc ctgcaaacat ttgaggccgg cttcgatgat ctcccacttt gaagaatcga
    16921 tcattattgg aattctggca atatccggtt cagaagcaat caggttgagg aattttacca
    16981 tagcaacttt tccatcgatc attccctcat ccatattcac atccaggatt tgggcgccac
    17041 cttcaacctg gtcacgcgca atctcaagcg ccgcttcgaa tttttcttcc tttataagat
    17101 ttaagaattt tcttgaaccg gtaacgtttg tcctttcacc aatgttcaca aaatttgttt
    17161 ccggtgttat gatgagcgga tcaagaccgc ttaaatgcat actagccatg cgtataaacc
    17221 tccttgtctc ctgtttttct tggctcaaat tc
//
LOCUS       DASWHX010000090         6588 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_438620, whole genome shotgun sequence.
ACCESSION   DASWHX010000090 DASWHX010000000
VERSION     DASWHX010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 6588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6588
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_438620"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..408)
                     /locus_tag="VGA21_06110"
     CDS             complement(<1..408)
                     /locus_tag="VGA21_06110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650113.1"
                     /translation="MKKYEIMNRLIWILLPMPLLLSNGCDDERVEQSCGCDGKATSIA
                     AWQDGSLYKNTDSAGSYLPKHDFGIWFYEHGEDYTSWPHYLLICNEDSISDFEDIPEF
                     SGIPVKFSGLLKDICGPSIENDTIHYIVLTGIEP"
     gene            719..1276
                     /locus_tag="VGA21_06115"
     CDS             719..1276
                     /locus_tag="VGA21_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018476663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX9650114.1"
                     /translation="MSDGGYKIRNKEGIHFVTFAVVEWVDVFTRKEYKDIVIESLKHC
                     QKERGLQIHSWCIMSNHLHLVVSAKQGDTSDILRDFKKFTSKEIIKAITNHAGESRRG
                     WMLKIFRREGAANSRNGTYQFWRQDNQPKELFSSEFTGQKLEYIHNNPVEAGIVEKAE
                     EYVFSSARDYYLGRNVGLLEIEFLD"
     gene            complement(1337..1579)
                     /locus_tag="VGA21_06120"
     CDS             complement(1337..1579)
                     /locus_tag="VGA21_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018475248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HEX9650115.1"
                     /translation="MKKDIHPKYKEVIFFDTSSEFKFMTRSTMTSSEKITWEDGKEYP
                     LIKIEVSSASHPFFTGKKLFVDTAGRVEKFNKRYKK"
     gene            1721..3388
                     /locus_tag="VGA21_06125"
     CDS             1721..3388
                     /locus_tag="VGA21_06125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650116.1"
                     /translation="MKSKFLILLMGIAPLAVHSQHTNLSLNEDYYGLVNRFHILQNGL
                     STDLHPAFRPYSQQFLRMKLDSLMTRVSKKEIFNAQYLKNDNGAVFGVESSRRPFLRH
                     FYKTKADLLGVKSDDLDLHANPVLYLSSGYASNTSTPFINTRGFEIRALIDNKVGFYT
                     FLTENQARFPEYTRNWISEINVVPHEAFWKDFKTSGVDFFTARGYISFNATKNINVQF
                     GQDRFFIGNGMRSMILSDFTAPYPFLKVNTRVWKFQYTNLFAQMKADAFGTLSGSAGT
                     RRYPNKYFAFHHLSFNVKKNFNIGIFESIMFGREDSLGNNGFEISYLNPVIFYRAAEQ
                     YNGSEDNVILGLDANYIFRNRFEVYGQFLFDEFLLDEITAGDGWWGNKFAFQVGGKYL
                     NVLGVDNLDLQVEYNQARPYTYSHRGIFTNYAHYMQPVAHPLGSNFREILANVRFQLL
                     PELTANARFIYYMKGVDPAGQNFGGNIIEPYNSRVSNYGNETGQGIRNTVAYSALNLS
                     FMLKHNLFLDGDFVYRTLDSDAPVQNSDDLILTLSMRLNIARRIHEF"
     gene            3415..5250
                     /locus_tag="VGA21_06130"
     CDS             3415..5250
                     /locus_tag="VGA21_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008631567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9650117.1"
                     /translation="MKTFLRIFSYSRPYSRVVPPFIIFTLLGVLFSVANFAMIMPLLD
                     VLFDKVEPDKIALYSQKPEFSFSLDFIIEYFNYYFIGLIENQGKSFGLLFICIILVVS
                     VFLTNFFRAIAGIVRTKWRTKILRDIRLELFQKVSRLHMGFFTNQKRGDVISRFSNDM
                     SEIDYTITYTLNNLFKEPVTLIVYFIVLFIISVKLTLFTLILLPITGGVIAEIVRRLR
                     RRAIQSQDSLGRIVNIVDETLGGMRIIKAFNARNFILKKFTREIKFFKRANDSIGIKS
                     EISSPISEFLGVSVVAGIIYYGGSLVLSENSVLSASEFITYIAIYSQVLNPSKAINKG
                     LSEIQKGLASAKRVFSLMDTNPEITEKPDAIQLKPFENCIEFRDVDFIYETEPVLKNI
                     NFKLEKGKTVALVGTSGGGKSTMADLVPRFYDPEKGSVLIDGIDIRDCTLDSIRAIMG
                     IVTQDSILFNDTVFNNIAFGSEEATLDDVIKAAKIANAHDFIEEMDQGYDSVMGERGT
                     KLSGGQRQRISIARAILKNPPILILDEATSALDSESEKLVQQALSNLMQNRTSLVIAH
                     RLSTIQHADEILVIQNGEIIERGTHDSLLLNKSGVYTKLHEIQKT"
     gene            5318..5680
                     /locus_tag="VGA21_06135"
     CDS             5318..5680
                     /locus_tag="VGA21_06135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650118.1"
                     /translation="MGRPKFIFFTSFFVFNLLLFLFALYVDANRLNIDVLFALQGQIP
                     YMKYVAALGVGLSIIAFGISFFVNQRHKEEIEKLKSEQKDYKARLYDMQEELKKLQNP
                     ELMANSPATPTGKSEKEK"
     gene            5770..>6588
                     /locus_tag="VGA21_06140"
     CDS             5770..>6588
                     /locus_tag="VGA21_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010609722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HEX9650119.1"
                     /translation="MVGKTFGSAVYGVEAKRISIEVNIVQGTKFYISGLPDNAVKESA
                     HRVESVIKTLGYYMPRQKVVVNLAPADLKKEGSAYDLPIALGVLAASGQLSDTEMEKF
                     LIMGELSLDGQLRSIKGVLPIAIEARKEGFKGFILPVQNAREAAIVKDLPVYGVADMS
                     EAIEFLKGYKKIEPVTVNPREVFANQSSQYDADFKDVQGQENIKRALEIAAAGGHNVI
                     MIGPPGAGKTMLAKRLPTILPPLTLLEALETTKIHSVAGKLGNQSSLVCNRPFRN"
BASE COUNT         1973 a         1206 c         1398 g         2011 t
ORIGIN      
        1 cggctcaatg cctgtcagca caatgtaatg aatggtgtca ttttcgatag atggaccgca
       61 tatatccttt agcaatcctg aaaactttac aggaatccca gagaactctg gaatgtcttc
      121 aaaatctgaa atggaatctt cgttgcatat caacaagtag tgcggccatg aggtgtagtc
      181 ttctccgtgt tcataaaacc atatcccgaa gtcatgttta ggaagatagc ttcccgcact
      241 gtccgtattc ttatacaaag agccatcttg ccaagctgca atagatgttg cttttccatc
      301 acatccacaa ctttgctcaa cacgttcatc atcacagcca ttgcttaata acaatggcat
      361 gggaagtaaa atccagatta atctattcat aatttcatat tttttcatgt atcttctaaa
      421 cttgctaata tgatatgtta gggtagagtt tatcagaaaa cttaacttta agatcatccg
      481 ggaaacccag gacaccagat tagtcaatta ttatggacaa tcaaatactg tattgtatta
      541 ttcaaagaaa aaagtctcaa aaaagcttgt tctaatgtat ggatgtaatt ccccagccat
      601 cgcagtttga ttttttgtgt tgattccttt ccggtttctg ctgccgttat tcaagggtac
      661 tttgattcaa taccgaaagc gtcattcctt tttaaaatta ctgcataaca gaaaagttat
      721 gtcagatgga ggttataaaa tccgaaataa agaaggtata cattttgtaa catttgcagt
      781 agtagagtgg gttgatgtat ttacacgaaa agaatataaa gacattgtaa ttgaaagtct
      841 taagcattgc cagaaagagc gaggattgca aattcatagt tggtgcatta tgagtaacca
      901 tttgcatctt gtagtttcag cgaagcaggg cgataccagt gatatacttc gggactttaa
      961 gaagtttact tcaaaggaaa ttatcaaggc aattaccaat catgccggag agagccggag
     1021 aggatggatg cttaagatat ttaggcgaga gggagcagcc aatagcagaa atggtacata
     1081 ccagttttgg agacaggata atcagcctaa agagctgttt agttcggaat tcacagggca
     1141 aaagttagaa tacatccata acaatccggt tgaggcgggt attgttgaaa aggcagagga
     1201 atatgtattc agtagtgcaa gagattatta cttaggaaga aatgttggtt tgcttgagat
     1261 agaatttttg gattgaattt ttttgtcctg agttgcaaac tcaggacagc gcattaattc
     1321 aatatagcta acacagttat ttcttatacc ttttgttaaa tttctcaacc ctgcctgcag
     1381 tgtcaacaaa taatttctta ccggtaaaga aagggtgaga agcggaactg acttcaattt
     1441 taatcaaggg atattccttg ccatcttccc aggtaatttt ctcgctggaa gtcattgtgg
     1501 agcgcgtcat gaatttgaat tcacttgaag tatcaaaaaa tattacttct ttgtatttag
     1561 gatgtatgtc ctttttcata gttatacctt aaaaagaggc acaaatttag ataaatcgga
     1621 attaaagaac aaaaatgagc aggcaaaagc atggtcaaat gaattcttcc cttatgttaa
     1681 accccctgaa atgtttaatt ttcgggttct ttgaaatttt atgaagagta aattcctaat
     1741 tctcctgatg ggtattgctc cgctggctgt ccattcacaa cataccaacc tgtctttaaa
     1801 tgaagattac tatgggttag tgaatcgttt tcatattctt caaaatggtc tttcaacaga
     1861 tttgcacccc gcgtttcgtc catactcaca gcagttctta agaatgaagc tcgattcttt
     1921 gatgacaaga gtgtcaaaga aggaaatttt caacgctcaa tacctgaaga atgataatgg
     1981 ggcggtcttt ggcgttgaat ccagtcgccg gcctttttta agacactttt acaaaacaaa
     2041 agccgacttg ctcggagtta agtctgatga cctggacctg catgcaaatc cggtcttata
     2101 tttaagtagc ggatacgcca gtaacacttc cacgcctttt attaataccc gtggattcga
     2161 aatccgcgcg ttgattgata acaaagttgg tttttatact tttttgaccg agaaccaggc
     2221 aaggtttccc gaatatacgc gtaactggat cagtgaaatt aatgttgttc ctcacgaagc
     2281 tttctggaag gatttcaaaa cttccggagt tgactttttt actgcacgcg gatatatcag
     2341 ctttaatgca accaaaaaca ttaacgtaca atttggtcag gaccggtttt tcattggcaa
     2401 tggaatgcgc agcatgattt tgtctgattt caccgcccca tatccatttc ttaaagtaaa
     2461 cacccgcgtt tggaaatttc aatacactaa cctgttcgcg caaatgaaag ctgatgcatt
     2521 tggaactctt tccggttctg ccggaacgcg gcgatatcca aataaatact ttgcttttca
     2581 tcacttgagt tttaatgtga aaaagaattt taacattgga atttttgaat ccatcatgtt
     2641 tggaagagaa gatagtttgg gaaataatgg ttttgaaatt tcctatttaa accccgtaat
     2701 cttttaccgg gccgccgagc aatataacgg aagcgaagac aatgttatcc tgggtttgga
     2761 tgctaattac attttccgga accggtttga agtgtacgga caatttcttt ttgatgagtt
     2821 tttgttggat gaaattactg ccggagatgg atggtgggga aataaatttg ccttccaggt
     2881 gggaggaaaa tatctgaatg tgctaggagt tgataatctt gatctgcagg ttgaatataa
     2941 ccaggcacgg ccatatacct attcgcatag aggtattttt actaactatg cgcattatat
     3001 gcagccggtt gcgcatcccc tgggatcaaa cttcagggaa attctggcta atgtccgctt
     3061 ccaactccta ccagaactta cggcaaatgc caggtttatt tactatatga aaggtgttga
     3121 tccggcgggt cagaattttg gcggtaatat aatagaacca tacaactcaa gagtttcgaa
     3181 ttatggcaat gagaccgggc agggtataag aaataccgta gcttattccg cactcaacct
     3241 ttcttttatg ctgaagcata acttgtttct ggacggtgat tttgtttatc gaacacttga
     3301 ttcagatgct ccggttcaaa atagcgatga tcttattctc acattatcaa tgcggttgaa
     3361 tatagcgcgc cgtatacatg aattttaata atatagccta actttgacgc tttcatgaag
     3421 accttcttaa ggatttttag ttattcacgg ccctacagcc gggtagttcc accctttatt
     3481 attttcacct tgcttggggt attatttagt gtggcaaact ttgccatgat aatgcctttg
     3541 ctggacgtct tatttgataa agttgagccg gacaaaatcg cgctttacag tcaaaaaccg
     3601 gaattctctt tcagcctgga tttcatcatt gaatatttta attattattt tattgggttg
     3661 attgaaaacc agggaaaatc ttttggtctc ctgtttattt gtataattct ggtagttagt
     3721 gttttcttga caaatttctt cagagccatt gccgggattg tgagaacaaa gtggcgaaca
     3781 aaaatattaa gagacatcag gcttgagttg tttcagaaag tgagtcggct ccatatggga
     3841 ttctttacca atcagaagcg gggtgatgtt atttcacggt tcagcaatga tatgagtgaa
     3901 attgattaca ccatcactta tacattaaat aacctgttca aggaaccggt caccttaatt
     3961 gtttatttca tcgtactttt cattatttcg gtgaagctga cacttttcac gctgatactt
     4021 ttaccaataa ccgggggagt gattgccgag attgtcaggc ggctgcgacg gcgtgcaatt
     4081 caaagtcagg actcgttggg gcggattgtc aatattgtcg atgaaacact gggcggaatg
     4141 agaattatta aagcatttaa tgcgcgtaat tttattttga agaaattcac ccgggaaatt
     4201 aaattcttta agagagcgaa tgattctatt ggtatcaaga gtgaaatttc ctctccaatc
     4261 tcagaatttc tgggagtatc agttgtagcg ggaattattt attatggcgg ctcgctggtt
     4321 ttgagtgaaa attctgtatt gagcgccagt gagtttatca cctacatcgc catttattcg
     4381 caggtgctga atccttctaa agccatcaac aaaggtttaa gtgaaattca aaaaggtctt
     4441 gcttccgcca aaagagtttt cagtttaatg gataccaatc ccgagattac tgagaaaccg
     4501 gatgctatac aactgaagcc tttcgagaat tgtatcgaat tccgggatgt tgatttcatt
     4561 tacgaaaccg aaccggttct gaagaacata aattttaaat tggaaaaagg aaaaactgtg
     4621 gctttggttg gaacaagtgg cggcgggaaa tcaacaatgg cggatttagt tccgcgattt
     4681 tatgatcctg aaaaaggttc tgtactaata gatggcattg acatacggga ttgtacactg
     4741 gattcaatcc gtgctataat gggtattgta acccaggatt ccatcctgtt caatgataca
     4801 gtctttaaca atattgcctt tggttcggaa gaagccactt tggatgacgt aataaaagca
     4861 gcaaaaattg caaacgctca tgattttatt gaagagatgg atcagggata cgactcagtg
     4921 atgggcgagc gtggcactaa actttcgggc gggcaacgtc agcgaattag cattgccagg
     4981 gcaatcctaa aaaatcctcc tatattaatt cttgacgaag ctacttcagc actggattcc
     5041 gaatcggaaa aacttgtgca gcaagcttta tcaaatctca tgcaaaacag gacttcactg
     5101 gtaattgctc acagactcag cactatacag catgcagatg aaatactggt aattcagaat
     5161 ggcgagatta ttgagagagg cacccatgat tccttattat tgaataaatc cggtgtgtat
     5221 acaaaattgc atgaaattca aaaaacctga tgtaaggaat attagtttca aaatttagat
     5281 ttacaatttt atcggattat gtttgttaaa tagataaatg gggcgtccaa aattcatatt
     5341 tttcaccagc ttttttgtat tcaacttgct cttgtttctc ttcgctttat atgtggatgc
     5401 aaaccgttta aacattgatg tattgtttgc tcttcaggga caaattcctt atatgaaata
     5461 tgtcgcggca cttggcgttg gtttgtccat tatagctttt ggcatttcct tttttgtaaa
     5521 ccagcggcac aaagaagaaa ttgaaaagct gaagtcggag caaaaagatt acaaggcccg
     5581 gctttatgat atgcaggaag aactgaagaa actgcaaaac cctgaactga tggccaactc
     5641 acctgccact cctacaggga agtcagagaa agaaaaataa acccaaatcc tgttacaagt
     5701 ctattttctt atttcttttc ttagattgca ttatattaat aaattattgc attttattat
     5761 ttgaaattca tggtaggaaa aacatttggc agcgctgttt atggagttga agcaaagcgg
     5821 atcagtattg aagtgaatat tgttcaaggt acaaaatttt atatctccgg cttacctgat
     5881 aacgcggtta aggaaagcgc tcatcgagtt gaatcggtaa ttaaaaccct ggggtattat
     5941 atgcccaggc aaaaggtggt ggtaaacctg gccccggcgg atttgaaaaa ggaaggctcc
     6001 gcatatgatt tacccattgc cctgggtgtg ctggcagcct ccggtcagtt gtcagatacg
     6061 gagatggaaa aatttctgat tatgggcgaa ctctcacttg atggtcagtt acgatctatt
     6121 aaaggagttc ttcccattgc cattgaagca agaaaagaag ggttcaaagg tttcatactc
     6181 ccggtacaaa atgcccgtga agccgccata gtgaaagacc ttcctgtata tggcgtggcg
     6241 gatatgtcgg aggccataga atttctaaaa gggtataaaa agattgaacc ggttacggtg
     6301 aacccgagag aagtgtttgc aaaccaatcc agtcaatacg atgcggattt taaagatgtg
     6361 cagggtcagg aaaacatcaa acgtgctttg gaaatcgcgg ccgccggtgg gcataatgtg
     6421 ataatgattg gccctcccgg tgcgggaaag accatgctgg caaaacgact gccaacgatt
     6481 cttccgccgc tgactttact tgaagcttta gaaactacaa aaatacattc ggtggcaggg
     6541 aaattgggta atcagtcatc gctggtttgc aaccggcctt ttcgaaat
//
LOCUS       DASWHX010000091        25214 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_808357, whole genome shotgun sequence.
ACCESSION   DASWHX010000091 DASWHX010000000
VERSION     DASWHX010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 25214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25214
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_808357"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..382
                     /locus_tag="VGA21_06145"
     CDS             <1..382
                     /locus_tag="VGA21_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011586063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEX9650120.1"
                     /translation="GNKERLVPIGKDAIKYTQLYIKEWRTNRSIVKGNEKYIFLNRRG
                     KKLTRVMIFTIIKNLARQTGLKKTISPHTFRHSFATHLLEGGADLRAVQEMLGHESIT
                     TTEVYTHLDRDYLKQVIQEFHPRS"
     gene            441..1106
                     /locus_tag="VGA21_06150"
     CDS             441..1106
                     /locus_tag="VGA21_06150"
                     /inference="COORDINATES: protein motif:HMM:TIGR02453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 domain-containing protein"
                     /protein_id="HEX9650121.1"
                     /translation="MTYFNNDFVKFFRELEKNNTTDWFHANKTRFENSVKKPFHKFID
                     DLIDALRFVHPNLDMTAKEAIFRINRDIRFSKDKSPYKTHMSALINEYGKKEYSKPAL
                     YVQANNKEVRVYSGSHDLDKLQLEKLRRHIVKNITEFNKLINAKPFKSMFGEILGDKN
                     KKLSPEFQKAAEKQPLLYNKSYYYFYKLPPSVLTGSKLIEEIVSGYKKAVPINKFLLK
                     GIK"
     gene            1155..3419
                     /locus_tag="VGA21_06155"
     CDS             1155..3419
                     /locus_tag="VGA21_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020532052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX9650122.1"
                     /translation="MTLINAAAIVKKNYRLDCESELLAGYLDKNFLIKTKQGEKFILK
                     LSQAPSELLEAQNSLLGYIDKRDPSLNVQKVVPANNHLLLQNEIEGEAWNIRLLTYLE
                     GQHFAEVNHTKGLMESLGGYLGRLTNAMNGFSHPEFKARTLEWDIQRTLLNRKYLDKI
                     SAPQDRKWVQYYMLQFSEIVVPLLPDLRHSVIYGDANDRNILVKDERVNSVIDFGDAV
                     YSQVINELAIAITYAVMGKEDPLSWAIPIVKGFHSEYPLEKNEIAVLYYLVAARLCIS
                     VCQSAKSKIEDPDNEYITVSEKPAWDLIRKWGKLNPGKVAKSFYQICGFSTEKTKSGK
                     EITELRAKHISKTLSLSYRHPIQMESAAFQFMFDSGGKTYLDLVNNIMHVGHCHPKVV
                     EAGRRQMAKLNTNTRYHYDLLTIYAENLCNYLPAKLNKIFLVNSGSAASDLAIRLAKN
                     YTGRDKVMVMDHGYHGNTIADIEISPYKYQGKGGFPQSDKIIKASMPDEYRFKGKVEE
                     FINEAKSQIDRSEKSISAFIVEPIVGCGGQVPLPRPYLKELFQHIKINGCLAIADEVQ
                     TGFGRVGTHFWAFEQQDAVPDILVLGKPIGNGHPLAAVITTPEIAGSFENGMEFFSSF
                     GGNPVSCAIGNAVLEVIKEEELQSNALAMGSKMLQLLKELQSRYPMIGDVRGSGLFLG
                     IELIRNENLDPATDGAKILINNMKDRGVLLSTDGPFNNVIKIKPPLCIDESDIDFFLN
                     NLDKELQSLSEEFK"
     gene            3429..4490
                     /locus_tag="VGA21_06160"
     CDS             3429..4490
                     /locus_tag="VGA21_06160"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015265627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HEX9650123.1"
                     /translation="MGANDFPLYKFLFRPLLFLLPPESAHNFTFFLLKVAYHLPFGRK
                     ILEATFCINDDSLKKKILGIEFKNPVGVAAGLDKNGEWIDELSSIGFGFVEIGTVTPL
                     PQSGNNKPRLFRLKKDLALINRMGFNNKGGRSVAERLRIRMPGIIVGGNIGKNKATSN
                     ENALSDYIKSMDLLYGNVDYFVINVSSPNTPGLRQLQEKEPLRRLLKGLKDHMNTKPT
                     YYSLWLKVAPDLRDEQVDEIVQIIQEAGVDGLIATNTTIDRKDLKTNEKIISNIDEGG
                     LSGMPLKDKSTEMIRMFRKKLGAGFPIIGVGGILSPKDAKEKIEAGADLIQLYTGLIY
                     EGPGLVRRINKYLKDTFQK"
     gene            4553..6976
                     /locus_tag="VGA21_06165"
     CDS             4553..6976
                     /locus_tag="VGA21_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013454790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HEX9650124.1"
                     /translation="MAKNTFKSNTFKKKTTEKKFSVKTGLFSDRKFHLAVGFFLMIVS
                     FFLFTSFISYLFTHEADQSVVDGISQTPVTDSGQEAENWFGLAGAVVSNAFIFKWFGL
                     ASFLFPPFIFLLSFEIIFRQSIYSLMRSFYIMIFFLFWISTLLGYLVFSSETVNEWGF
                     LSGGIGYEISFFLDSLMGWGTYLLLSFLLIVFIIFFFNITSIFLFRTAPAVNPGYKNG
                     ENVLINGNLAGPELWEEEKEDDAELVSAMEDGKHEAENEIEVEDSLSGEKKTLELDIE
                     ENPEPVQEEPAFFVAEPAKEAVSTKLENYDPTLDLPKYKYPTIDLLNEYNASKVQVTK
                     EELEQNKDRIIETLVNFKIGISSIKATIGPTVTLYEIVPEAGIKISKIKNLEDDIALS
                     LSALGIRIIAPIPGKGTIGIEVPNKNREMVDMRSVIATEKFMKSDKELPVALGKTISN
                     ELYVVDLTKMPHLLMAGATGQGKSVGLNVILTSLIYKKHPSQLKFVLVDPKKVELTLF
                     NKIERHFLAKLPNHEEAIVTDTKRVVETLNSLCIEMDQRYDLLKEAGTRNIKEYNQKF
                     VNRKLNPQKGHRFLPYIVLVVDELADLMMTAGKEVEGPIARLAQLARAIGIHLVIATQ
                     RPSVNVITGLIKANFPARLSFKVTSRVDSKIILDTGGAEQLVGMGDMLLSQGSEINRL
                     QSPYVDTSEVETICEFIGSQRGYDDAYQLPEFIGDEGGDNISDVDLSERDALFEDAAR
                     IIVLHQQGSTSLIQRKLKLGYNRAGRLIDQLEAAGVVGPFEGSKAREVLIPDEYSLEQ
                     LLSNLDEKN"
     gene            6997..7644
                     /locus_tag="VGA21_06170"
     CDS             6997..7644
                     /locus_tag="VGA21_06170"
                     /inference="COORDINATES: protein motif:HMM:NF015510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HEX9650125.1"
                     /translation="MLKRITLAIGLLLLAKITFAQYDPKAFEILQAVSNKYKDIPVFK
                     AKIIYSLINESEGINDNFQGEIVIKGEKFRLLMEEQEIVNNGEFVWTYIPDVNEVSID
                     NYDPETNDINPTKILNAYKEGFKYLYLNDVAAGGEIYQVVDLVPEDPKNSQYFKIRLQ
                     VNKKDSMLKSWTMFDKSGNKYNYDVENFSTNLNPEDNFFVFDVKKHKGIEIIDLR"
     gene            7699..8526
                     /gene="pyrF"
                     /locus_tag="VGA21_06175"
     CDS             7699..8526
                     /gene="pyrF"
                     /locus_tag="VGA21_06175"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008630032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEX9650126.1"
                     /translation="MNIQFLFNQIKAKESYLCVGLDTDIHKIPQHLLQHSDPIFEFNK
                     RIIDATKDLCIAYKPNIAFYESLGARGWESLRKTLEYIPEEIFVIADAKRGDIGNTSK
                     LYARAFFENLRTDAITVSPYMGEDSVKPFLDFPEKFVILLGLTSNPGSNDFQRMKMES
                     GNMLFEEVLMKSQQWGGPHKIMYVIGATQPQFISKVREICPDHFLLIPGIGVQGGSLK
                     EISEVGMNSKVGLIVAVSRSIIYASAGKNFALKARLEAKKLQEEMAHYLSLHFKNYR"
     gene            8595..9869
                     /locus_tag="VGA21_06180"
     CDS             8595..9869
                     /locus_tag="VGA21_06180"
                     /inference="COORDINATES: protein motif:HMM:NF022466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2851 family protein"
                     /protein_id="HEX9650127.1"
                     /translation="MTEETLSYLWRFQRFSRQNLKTSDGKQLTIYNPGTLNSDSGPDF
                     SDARIRLDTIEWCGNIELHVNSSDWRKHGHQNDEAYRNVILHVVWNHDQEVNIGDYKI
                     PVLELKGTVAVSLLSKVETLIKNITDIPCSSFRIDWSGVHQLSALENATVQRLQRKCA
                     EIQLELESLKGDWEEAFYRRFTANLGFKVNKDPFYALAKGVSQKLIAKYSFSPPKVES
                     LLMGVAGFLNQRIHDEYYLGLRTEFNFLQKKHNLSPIDPGWKFMRMRPANFPTVRISQ
                     LAAIYDHNQNLFRKLIEISNYSDIVKILRVETADYWKSHYLFGEESKSRNTALGKASI
                     DLITINTIVPFLFSYGVINDLNEFKDKAINLLQAIQPEKNRITKRWGKAGLKLNSAFS
                     TQGSIELFENYCRLKKCLQCKIGVTCLSENQS"
     gene            9870..11039
                     /locus_tag="VGA21_06185"
     CDS             9870..11039
                     /locus_tag="VGA21_06185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650128.1"
                     /translation="MEINSLVTPKNFLFTVGLLIKLYCALVIIYLYIFHYGMGDMISY
                     QVQSHKILPEFYANPITFLLKSDLGFSDSIYFGQPRAIFFLKCIVVSSLITGGDIYLT
                     TFLFSIFSFIGSYRVFAAIYDYYPKVTYEAALAFLLLPTYILWTSGMLKESLANGAIC
                     LMVAEVLHLVHKKESRIFSLLILTISGWLLIKLKYYYFGALLPVLFVYFFYKRWGQYL
                     SNKKISIRISVFLGLLLIGSLAGMVFHEYLNPLLWFDIISESNQSYLATKSAGNLWQI
                     ITQIPLAIYNSWLAPQLWQAKNFVQFLSAAESTFFGLILVLSIYKNPNPFKWSLEAWV
                     IVAYSLILGIMLGLSITNFGSLERYRTSFYPFLFMLVASGLPQVKSLIKAIFEKI"
     gene            11047..11685
                     /locus_tag="VGA21_06190"
     CDS             11047..11685
                     /locus_tag="VGA21_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009581190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEX9650129.1"
                     /translation="MAKPVIIFGANGIGKAALEIFRSSGIDVLGFLDDDENLFGKEVN
                     NIPVLGKTGNEEYLKLLKEKSESFVALDETILRKKQIETLVNEYKSMPINAIHSSAII
                     PDSVHIGHGNFINAGVIIGSYAKVPNHCLLHSGTIIEHEAQLGNFTQIGAGSIINPGV
                     IIEEGVFIGSGVTIVSGIIVRKNAKIGAGSVVVSNVEKSQVMFGNPAKQVSA"
     gene            11738..12592
                     /locus_tag="VGA21_06195"
     CDS             11738..12592
                     /locus_tag="VGA21_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019948885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HEX9650130.1"
                     /translation="MPISETNLILNSDGSVYHLNLKPEDIADTIIAVGDPSRVYSVSN
                     YFDDVDFEMNRREFITHTGRYKGKKVTVISTGIGTDNIEIFFTEIDALVNIDLKTREV
                     KTKKNSLKIIRVGTSGALQEDIPVDSHLASENAIGIDSLMCFYNLPQNSLEQKLGESL
                     KVKLSLPYAPYVVSASKELFKKFGKDMLVGNTVTCPGFYAPQGRKVRLDLKIPNLFNE
                     MNYFHENGMWLTNFEMETAAYYAFGRLMGHETLSLNAIIANRITNKFSKDPEKSVDSL
                     IRKVLDRI"
     gene            complement(12589..12846)
                     /locus_tag="VGA21_06200"
     CDS             complement(12589..12846)
                     /locus_tag="VGA21_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009581192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650131.1"
                     /translation="MKTVKTTFRLTYTEDQYKRSMDFVEDMKRHPRRVYWLGKEGKSD
                     EELVISHIAHRILSGFYNNYNPSYTHSQILAMESMTNGNNN"
     gene            complement(12997..13488)
                     /locus_tag="VGA21_06205"
     CDS             complement(12997..13488)
                     /locus_tag="VGA21_06205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650132.1"
                     /translation="MKNKQEGKAEKLFKKLGKRLDGMIEELSDLKKDAGKEFGKRMEE
                     VKRNKETLETELKDFGNKHKHKWGEIEDSLEKAAVELRKAFSVAFSGRKKSEKASADS
                     ETSSDKTSPNGANPDTGGPAENKKKKKSKKKQAAKKPESKKEKKKPSGSGNKDSKKSK
                     KGG"
     gene            13533..14210
                     /locus_tag="VGA21_06210"
     CDS             13533..14210
                     /locus_tag="VGA21_06210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650133.1"
                     /translation="MESNNAIDQLHNLLKIKAGVKQLSYRNVKQSFEMLRTEAISIVQ
                     ELSESIRPDDTSIHVEYKDQGEFEFHVKFSGDLLIFMMHTNIITFDGEYHLLKSDYIS
                     EKENRKYFGHIMIYNFMADSLKYNRLEDPGYLVARMLVNCENHFMIEGVKPLNFLFPE
                     IKANKVSAPMLRYFIEKAMIAAIDNDLIAPPYPKLKTISLFEKVNQNQELGRGQKIGF
                     KLSMSSD"
     gene            complement(14207..15829)
                     /gene="ade"
                     /locus_tag="VGA21_06215"
     CDS             complement(14207..15829)
                     /gene="ade"
                     /locus_tag="VGA21_06215"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009578094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HEX9650134.1"
                     /translation="MEIKGRIIDIEGKKIFKGVVRLSEGKIISVEEINADVPEQFILP
                     GFIDAHVHIESSMLTPSEFARLAVVHGTVSTVSDPHEIGNVLGKAGVMYMIEDGKKTN
                     FKFYFGAPSCVPATPFETAGSTISVNEIEELFQLNEVKYLAEMMNWPGVLNNDPEVLA
                     KISLAHKMNKQVDGHAPGLKGGQAKKYIDAGITTDHECFTKEEALDKLSFGMKVIIRE
                     GSAARNFDALADLLHEHYENMMFCSDDKHPDSLVEGHINKLVERALAKGVDLFKVLQA
                     ACLNPITHYGLGVGRLRPGDPADFIIVDNFKRVNVLRTYINGINVAENGTTKLSRVLS
                     PIVNNFNTSPIDAGSLALKSSVNQIRVIEAHDGELVTTSGIEQATKIDGNIVPDVSRD
                     ILKLVVVNRYENSRPAIGFIKNFGLSRGAIASSVAHDSHNIIAVGTNDNDIAKVINLV
                     IEEKGGIAAINGVDSSILPLPIAGLMSDLDGYEVAKKYSALDVEAKQMGSRLSAPFMT
                     LSFMALLVIPELKLSDQGLFDGIKFEFVDVEC"
     gene            15887..16564
                     /gene="cmk"
                     /locus_tag="VGA21_06220"
     CDS             15887..16564
                     /gene="cmk"
                     /locus_tag="VGA21_06220"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015265824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEX9650135.1"
                     /translation="MKNIIVAIDGHSGCGKSSTAKAVARRLNFTYIDSGAMYRAAALY
                     LKRLNINPDEREKIKESISEIRIDFEKDANSNFTTILNGENVEEEIRSMEINRLVSRY
                     SVVPQLREAMVSNQRMLSKNRNVIMDGRDIGTIVFPNADLKFFMTADLKVRALRRQNE
                     LAAQGKIENLDVVMQNLAHRDEIDSTRESSPLIQADDAMVIDTTNLTMEHQIEMVVNL
                     AAERMKE"
     gene            16719..18083
                     /locus_tag="VGA21_06225"
     CDS             16719..18083
                     /locus_tag="VGA21_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015265826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit A"
                     /protein_id="HEX9650136.1"
                     /translation="MSQFIKLKRGFDINLAGKASNKVENPTQPEKFAIKPFDFMGLKR
                     PKLLVEIGDRVLAGQPLMYDKMFEGVNYVSPVSGEVLDIVRGKKRVLLEIVIKSDNEF
                     KCLQFKKYSVSDINNLDRQTIIEAMSKTGVWPNMIQRPFGIVADPKTEPKSIFISGFD
                     SHPLAPDYGVLLKDHQKELQAGINVLRKLTSGLIHFNVNGSGEMPTVYSALNNIQLNK
                     FTGPHPVGNVGVQIHHIDPISKGDIVWTVNPVGVVQIGTLFLEGIYDASKLVAFVGSE
                     VAEPKYYKTYSGACIDTIARENIRNSHVRFVSGNALTGKRIEPAGFLGFYDHMITVLP
                     EGDKAEFLGWITPTFQKLSFQRAFGLLSFLNSKKEFALSTNARGEPRAFVQTGVFEKV
                     TPMDIFPLYLVKAIMAEDFDDMEALGIYEVIEEDLALCEFVDVSKHDIQDILRQGIEL
                     IQTS"
     gene            18087..19289
                     /locus_tag="VGA21_06230"
     CDS             18087..19289
                     /locus_tag="VGA21_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010611293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit B"
                     /protein_id="HEX9650137.1"
                     /translation="MKFLKNLLDKQRPLFEKGGKLERFYLLFEAGETFLFSPSETTKP
                     RGVQIRDAIDLKRLMMTVIIAMLPCLLFGIWNVGHQHFIAVGELNASAADKWLIGLRV
                     VIPILLVSYIAGGLVEVVFVVIRKHPLNEGYLVTGMLIPLVVPPTIPLWQVALAAIFA
                     VLIAKEAFGGTGMNVLNVALTARAFLYFAYPSQISGDVWTYLGDKTPVDGYSGATPLA
                     LTNQAITTGESVVTSLNNFGAGWHEGVYRFSSMFFGFLPGSIGETSTLMCLIGAAILI
                     TTGVGSWKVMLGVIIGAFAMGNFVNLLAVNAYMEMPAYYHLVIGGLAFGTVFMATDPV
                     TAAQTERGKWIYGIFIGILTVIIRVFNPAYPEGIMLAILLMNVFAPLIDHYVVQGNKK
                     RRLKRATI"
     gene            19276..20040
                     /gene="nqrC"
                     /locus_tag="VGA21_06235"
     CDS             19276..20040
                     /gene="nqrC"
                     /locus_tag="VGA21_06235"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014772258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit C"
                     /protein_id="HEX9650138.1"
                     /translation="MRRSNGYIIIFTAILTIVVGGLLSLTSLVLGPAQKKSIELDTKS
                     QILSAVMQIQKGDDILDIYRKRIQSVVVDARGNEITTDEKGKPIQAENIDIAKNFKKT
                     RENRQYPVFKFMNKENELQVDAYILPIYGNGLWDKVWGFVAVDEKFESIVGVSFAHKA
                     ETPGLGARIATTEIQTRFEGKKILNEAGEVVSVTMQKGEKGGGQASIDAFAGKPHQVD
                     GMSGATLTAKGVNNMMLDYLTSYKPYFEKVKSQVSL"
     gene            20090..20764
                     /locus_tag="VGA21_06240"
     CDS             20090..20764
                     /locus_tag="VGA21_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019596818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit D"
                     /protein_id="HEX9650139.1"
                     /translation="MSTETVEQVQEVLVKKVEPLFSKRRKKFVTDPLNEDNPITVQVL
                     GICSALAITIKMEPTLIMSIAVVFVIVFSNLIISVMRNLIPNRVRIIVQLVVVATLVT
                     VVSEILKAYYFEMYKVLGAFVGLIITNCIVMGRLEAFAMGNKPWDSVLDGMGSGFGYA
                     WIILTVAFFRELFGSASLFDFKIFSAIGLSGFPTNAMMVDSIGAFMILGLLIWAQRSK
                     TGYVEH"
     gene            20779..21390
                     /gene="nqrE"
                     /locus_tag="VGA21_06245"
     CDS             20779..21390
                     /gene="nqrE"
                     /locus_tag="VGA21_06245"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010611290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit E"
                     /protein_id="HEX9650140.1"
                     /translation="MELINLGIKAIFIENMIFAYFLGMCSFLAVSKKVNTAIGLGAAV
                     IFVLTLTVPLDWLIQEYILREGALAWIGPQFEEISLEFLQFIMFIAIIAAIVQLVEMI
                     IEKISPALYSTLGIFLPLIAVNCAILGSSLFMVQRDYTLAEATVFGFGSGVGWFLAIV
                     ALAAIREKLKYSNVPDGLKGLGITMLITGLMGIAFKSFIGIAL"
     gene            21512..22078
                     /locus_tag="VGA21_06250"
     CDS             21512..22078
                     /locus_tag="VGA21_06250"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX9650141.1"
                     /translation="MDDKEIYDRIKSDKQNFAILYDQYFVPIKKYILRRIGNYDISCD
                     VTAETFLKAYLKISHFEYRGISYKVWLYRIATNEVNLFFRSKKYKPLNKINLEDFNNE
                     TLITSQQEDKDIWEKELQDHHDFLKAQQVIKNLPIKYAEVLSLRYFEKASIKEIAEIL
                     DKPKGTIKSLCTRGLQKVKEKMQPGTES"
     gene            22102..22575
                     /locus_tag="VGA21_06255"
     CDS             22102..22575
                     /locus_tag="VGA21_06255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650142.1"
                     /translation="MKNMEDLPVPDPELSPDQNLKKTILNTQTSARIGIPLLILPGIL
                     LISMILKEELGINLGIINAITELIGNMDKSPYTGWLSPLIFLLLPLMAIIIFLLSITH
                     FSYNKENYILQINIKFKTFNIMLLLIAGAILGTITLYLILENLNHFMLDKFGQPL"
     gene            22696..22836
                     /locus_tag="VGA21_06260"
     CDS             22696..22836
                     /locus_tag="VGA21_06260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650143.1"
                     /translation="MRKILQLVAIALVAFECTQPAEKAIDDSIFTPMIGDYYQGLLEL
                     YL"
     gene            22876..23523
                     /locus_tag="VGA21_06265"
     CDS             22876..23523
                     /locus_tag="VGA21_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009577507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta family hydrolase"
                     /protein_id="HEX9650144.1"
                     /translation="MGRKISFKVSDKSGSVTGIYEKSGKGDRLLVLGHGAGAPMHSTG
                     MQLIAETLASKGISTLRYNFPYMEKGIKRPDVPAVAQLTVRKAVEKAKSLEPGAKIYA
                     GGKSFGGRMTSQAESEELLGVEGLVFYGFPLHAPGRDGVERADHLNQVKIPMLFLQGT
                     RDALANIALMKKVCKELKKSKLEIIDGGDHSFKVPKTDPNAVFENLASLVANWMK"
     gene            complement(23507..24700)
                     /locus_tag="VGA21_06270"
     CDS             complement(23507..24700)
                     /locus_tag="VGA21_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014019960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase"
                     /protein_id="HEX9650145.1"
                     /translation="MKTKIGLLLCLTLTFCIPKHQVSQQNWFKGNLHTHSYWSDGDDY
                     PEMVMDWYKSNGYDFVSLSEHNVIAEGEKWKLIPKSRVHEEAFQKYLEKYGEEWVNFK
                     EDTGRISVKLKTLNEYRKLFDASGEFLILQAEEITDRFDGKPLHLNANNLQELIPPQG
                     GNSVTEVLQNNINTVIEQRARLGVPIMVHVNHPNFGYAITVEDLIPVRGERFFEVYNG
                     HAAVNNRGDSAHISTEQMWDLINSARIKSGDALLFGLATDDAHNYHLFGSSYSNAGRG
                     WVMVNCDELTPETLINAMEAGQFYSTTGVILKKLTFEDNLLSIAIRPESDVTYEFEFI
                     GQFAGESPKILHRQSATEVQYQLTEDVEYVRARITSSKLMENPVYEKEQEMAWTQPVR
                     FTSSN"
BASE COUNT         7756 a         4767 c         5298 g         7393 t
ORIGIN      
        1 aggaaataaa gaaaggttgg tgccaattgg aaaggatgca ataaagtaca ctcagcttta
       61 cataaaagaa tggcgaacca acaggtcaat tgttaaggga aatgaaaaat atattttcct
      121 gaaccgccgc gggaaaaaac taacccgtgt catgattttt actatcataa agaacctggc
      181 ccgtcaaacc ggattaaaga aaaccattag cccgcataca ttcaggcatt cgtttgcaac
      241 tcatttgttg gaaggcggtg cggatttacg cgctgttcag gaaatgctcg ggcatgaatc
      301 tattaccacc accgaggttt acactcacct ggacagggat tacctgaagc aggtaatcca
      361 ggaatttcac cccagaagtt agttagggtt ttataatcaa aacaatgaaa tttacgtaat
      421 acctaataaa atcattttta atgacttact ttaataacga ttttgtgaaa tttttcaggg
      481 aactggaaaa gaataacact accgactggt ttcatgcgaa taaaactcgt ttcgagaatt
      541 cggtgaaaaa acctttccac aaattcatag acgatttgat tgatgcactc aggttcgttc
      601 atccaaatct ggatatgacc gccaaagaag ccattttcag gattaatcgt gatattcggt
      661 tttctaaaga caagtctcca tataaaactc atatgtccgc actaataaat gaatacggga
      721 aaaaagaata ttccaaacct gctctttatg tacaggcaaa taacaaggaa gttcgtgtct
      781 attccggatc gcatgatttg gataaacttc aattggaaaa gctcaggagg catatcgtaa
      841 aaaacatcac tgaatttaat aaacttatta atgcaaaacc gtttaaaagc atgttcggag
      901 aaatacttgg agataagaac aaaaaactgt cacccgaatt tcaaaaagca gcggaaaagc
      961 agccattgct ttacaataaa tcctattact atttctataa gttgcctccc tcggttctta
     1021 ctggcagtaa actcattgaa gaaatagttt caggatataa gaaagccgtc cccataaaca
     1081 aatttctgct gaaaggaatt aagtaaaatc ccttacttca ataattgtgc aaggctgctt
     1141 atcttagttg caaaatgact ttgataaacg ctgctgccat agtaaagaaa aactaccggc
     1201 ttgactgtga atctgagctg ttggcaggct acctagataa gaattttcta atcaagacaa
     1261 agcaaggtga aaaatttatt ctcaagctca gccaggctcc atccgaactc cttgaagctc
     1321 agaattccct ccttggatat attgacaaaa gagatccatc gctgaatgtt caaaaggttg
     1381 ttccagccaa taatcacttg ttgcttcaaa atgaaattga aggtgaagcc tggaatatca
     1441 ggttattgac ttatctggaa gggcagcact ttgctgaagt taaccacacg aagggtttaa
     1501 tggaatcatt gggaggctac ctcggacggc taacgaacgc catgaatggt ttttctcacc
     1561 cggagttcaa agctcgcact cttgaatggg atatccaaag gactttgctg aaccgtaaat
     1621 atcttgataa gatatcagct cctcaggata ggaaatgggt tcaatattat atgctgcaat
     1681 ttagtgagat agtagttcca ctattaccgg atctaagaca ttcggttatt tatggtgacg
     1741 cgaatgaccg gaatatttta gtcaaggatg aacgcgttaa ctcggttata gatttcggtg
     1801 atgccgtcta cagtcaggta ataaatgaac tggcaattgc aattacttac gcggttatgg
     1861 gaaaggaaga tcctttaagc tgggcaatac caattgtgaa aggttttcat tcagaatacc
     1921 cgctggagaa aaatgagatt gccgtgcttt attatttagt tgccgcaaga ttatgcataa
     1981 gtgtttgcca gtcagcaaaa tcaaaaattg aggatccgga taatgagtac attactgtca
     2041 gcgaaaaacc agcctgggat ctgattagaa aatggggaaa acttaaccct ggaaaagtgg
     2101 cgaagtcgtt ttatcagatt tgtggatttt cgacagaaaa aacgaaatcc ggtaaagaaa
     2161 ttactgagtt aagagcaaag cacattagca aaaccctttc cctaagttat aggcatccca
     2221 ttcagatgga gtcagcagcc tttcaattta tgtttgattc tggcggaaag acctacctcg
     2281 acctggtaaa caacataatg catgtaggcc attgtcatcc aaaggtagta gaagcgggcc
     2341 ggcgacaaat ggcaaagctc aatacgaata cccggtatca ttatgatttg ctcaccattt
     2401 acgctgaaaa tctttgcaat tatctgccag cgaaattgaa taagattttt ctggtaaact
     2461 ccggtagtgc tgccagcgat ttggcgattc ggctggcaaa aaattacaca gggagagata
     2521 aagtaatggt aatggatcac ggatatcatg gtaatacaat cgcggacatt gaaataagtc
     2581 cctataaata tcagggcaaa ggaggcttcc ctcagagtga caaaattatc aaagcatcta
     2641 tgccagacga atataggttt aagggaaaag tggaagaatt tataaatgaa gccaagtctc
     2701 aaattgatcg atctgagaaa tccatctctg catttattgt ggaacctatt gtgggatgcg
     2761 ggggacaggt tcccctgccg agaccttacc tcaaagaatt atttcaacac ataaaaataa
     2821 atggttgtct tgccattgca gatgaagtac aaaccggttt cggcagggtg ggaactcact
     2881 tttgggcctt tgaacaacag gatgcagttc ctgatattct ggtgttggga aaaccgattg
     2941 gcaacggaca tccattggcg gcagttataa caactcctga aattgccggt tcatttgaaa
     3001 atggaatgga gttctttagc tcatttggag gaaaccctgt ttcctgcgcc attggaaatg
     3061 ccgtgctgga agtaataaag gaggaagaac ttcaatccaa tgcattggca atgggttcga
     3121 aaatgcttca attgttaaaa gaattgcaat caaggtaccc aatgattgga gatgttcggg
     3181 gttcaggatt atttcttggg attgaattga tcaggaatga aaatcttgat ccggcaacgg
     3241 atggagcaaa aatactaata aacaacatga aagatcgggg agtacttctt agcactgatg
     3301 gcccctttaa taatgtgata aaaattaaac cgccactctg cattgatgaa tccgatattg
     3361 acttcttttt aaataatctg gacaaagaac tccaatcgct gtctgaagaa ttcaaataaa
     3421 ctttatcttt gggagcaaac gacttcccat tgtataagtt tcttttcaga cctctattat
     3481 tcttactacc gcctgaatct gctcacaatt tcactttttt tctgcttaag gttgcttacc
     3541 atcttccttt tggaagaaag attcttgaag cgacattctg cattaatgat gattcattga
     3601 aaaagaaaat cttaggcatc gagtttaaaa atccggttgg cgttgccgca ggtcttgata
     3661 agaatgggga atggattgat gaactgtctt caattggatt tggctttgtt gagattggaa
     3721 ccgtaactcc gcttcctcaa tcgggcaata ataaaccgcg gttattcagg ttgaagaaag
     3781 atcttgcatt gattaacaga atgggcttta ataacaaagg aggtcgctca gtggcagaac
     3841 gattaagaat caggatgccg gggataatag tgggtggtaa tattggaaag aataaagcaa
     3901 cgtcaaatga gaatgcttta agcgattata tcaaatcaat ggatttgtta tacggcaatg
     3961 tggattactt tgttattaac gtaagctcgc ctaacacacc gggtttacgt caacttcagg
     4021 aaaaagaacc tttacggaga ttgctgaaag gacttaagga tcacatgaac acaaaaccaa
     4081 cctattattc tttatggctg aaagtggcgc ccgatttaag ggatgaacaa gtggatgaaa
     4141 tagttcaaat tattcaggaa gcaggtgttg atggcctgat tgcaaccaat accacgatag
     4201 accggaaaga tcttaagaca aatgagaaga ttatttccaa tattgatgaa ggtggattaa
     4261 gcggaatgcc tttaaaagat aaatcaacgg aaatgatccg gatgttcagg aagaaattgg
     4321 gagccgggtt tccaattatc ggcgttggcg gaattttatc accaaaggat gcaaaagaaa
     4381 aaatagaagc tggagcggat ttaattcagt tatacacagg tttgatttat gaaggaccgg
     4441 ggcttgtaag gagaattaac aaatatctga aagatacatt tcaaaaatag ttttgcctat
     4501 caattcaatt caattaatat taattctgaa ttgattaatt ttacagacgc aaatggctaa
     4561 gaataccttc aagagcaata cgttcaaaaa gaagaccact gaaaagaagt tctctgtgaa
     4621 gaccggtctt ttctctgatc gaaaattcca tctagccgtt ggtttctttt taatgatagt
     4681 atcctttttc ctttttactt cattcatttc ttacttgttt acccatgaag ctgatcaaag
     4741 tgtggtagat ggtatatcgc aaaccccggt gacggattca ggtcaggagg ctgaaaactg
     4801 gtttggttta gcaggagcgg tagtttccaa tgcatttatt tttaaatggt ttggtcttgc
     4861 atcttttctg tttccgcctt ttattttcct gttaagcttt gaaataattt ttcgtcaaag
     4921 catctattcc ctgatgcgct cattttacat tatgatattt ttcctctttt ggatcagtac
     4981 gttgctgggg tacctggtat tttcctccga aacggtaaat gagtggggat ttctcagcgg
     5041 tggaatcggt tatgaaattt cattcttttt ggatagcctg atgggttggg gaacttattt
     5101 gcttctatct tttttgttga tcgttttcat catcttcttt tttaacatca ccagtatttt
     5161 tctcttcaga acagcacctg ctgtaaaccc tggttataag aatggagaaa acgttctgat
     5221 aaacggtaac cttgcagggc ctgaactttg ggaagaagaa aaagaggacg acgcggaatt
     5281 ggttagtgca atggaagatg gaaagcatga agctgaaaat gaaattgagg ttgaggacag
     5341 tctatcaggg gaaaagaaga ctttggaatt ggatattgaa gaaaatccgg aaccggttca
     5401 ggaggagccg gcattttttg tagcggaacc ggccaaagaa gcagtctcaa ccaaattgga
     5461 aaattacgat cctaccctgg atctgcctaa gtataaatat cctaccattg acctgcttaa
     5521 tgaatataat gcttccaagg ttcaggtaac caaagaagaa ctcgagcaga ataaggatag
     5581 aatcattgaa actcttgtga attttaagat cgggatctcg agcattaagg ctaccatcgg
     5641 acccaccgta acactatatg aaattgttcc ggaagccggt atcaagattt caaaaataaa
     5701 aaatctcgaa gatgatatcg cccttagcct gtctgcatta ggtataagaa tcatcgcgcc
     5761 aatcccgggt aaaggaacaa taggaattga agttcctaac aagaaccgcg aaatggtgga
     5821 tatgcggtcg gtaatcgcga ccgaaaaatt catgaagagt gataaagaat taccggtagc
     5881 gttgggtaaa acaatttcca atgaacttta tgtagtagac ctcaccaaaa tgcctcacct
     5941 gctgatggcc ggtgcgactg gtcagggtaa atctgtcggg ttgaatgtga tcctgacttc
     6001 tttgatctat aaaaagcacc catcgcagct aaaatttgtg ctcgtagacc ccaagaaagt
     6061 ggagcttact ttatttaata aaattgaacg tcatttcctg gctaaacttc caaaccatga
     6121 agaggctata gtaacggaca caaaaagggt tgtagaaacc ctgaattcgc tctgcattga
     6181 aatggatcaa cgatatgatt tgctgaaaga agcggggaca agaaatatca aagagtataa
     6241 ccagaagttt gtcaatagaa aactgaatcc gcaaaagggg caccgctttc tgccatacat
     6301 tgtactggtg gttgatgagc ttgccgatct tatgatgaca gcgggtaaag aggtagaagg
     6361 tccaattgca aggttggctc agcttgcaag agccatcggg attcacctgg taattgcaac
     6421 ccagagaccg tcagttaatg taatcaccgg cctcataaag gctaatttcc cggcaaggct
     6481 ttccttcaaa gttacttcgc gcgtagattc aaaaattatt ctggataccg ggggcgcgga
     6541 acaacttgtg ggtatgggag atatgctttt gtcacagggc tctgagataa accgccttca
     6601 aagtccctac gtggatactt cagaggttga aacaatctgt gaatttattg gtagtcaaag
     6661 aggttatgac gacgcctacc aacttccgga gtttatcggc gatgaaggtg gagacaatat
     6721 cagcgacgtg gacctttcgg aaagagacgc gttgtttgag gatgctgccc gaattattgt
     6781 attgcaccag caaggttcaa cctcactgat tcagagaaaa ctgaagctgg gctataacag
     6841 ggcaggaagg ctgattgatc agcttgaagc ggccggcgtt gtcgggccct ttgagggtag
     6901 taaagcacgc gaagtgctta ttcctgacga gtacagtttg gagcaattat tgagtaatct
     6961 ggatgaaaag aactaacttt gcgcaaattt caatccatgt taaaaagaat tacccttgcc
     7021 attggattat tacttcttgc caaaataact tttgcgcagt atgatcctaa agcattcgaa
     7081 atattgcagg cagtaagcaa taaatacaaa gacatccctg tttttaaagc caaaataata
     7141 tactcgctca taaacgaatc cgaaggaatc aatgacaatt ttcaaggaga aattgttatc
     7201 aaaggagaaa aattccgtct tctaatggaa gagcaagaga tagtgaacaa tggcgagttt
     7261 gtctggacct acatcccgga tgttaacgag gtgagtatcg ataactacga tcctgaaaca
     7321 aacgatatta atcccaccaa aattctgaat gcttataaag aaggtttcaa atatttgtac
     7381 ctgaatgacg ttgcagcagg tggggaaatt tatcaggtgg tagacctcgt ccccgaagat
     7441 ccaaaaaata gtcagtactt caagattcga ttacaggtaa ataagaaaga ttctatgctg
     7501 aaatcctgga ctatgtttga caaatcgggg aataagtaca actatgatgt tgaaaatttt
     7561 tctacaaacc ttaaccctga agacaatttt tttgttttcg atgttaaaaa acataaagga
     7621 atcgaaatta ttgatctgcg gtaaaaagaa atttctaaac agaaagccgg gttatgcgcg
     7681 gcttttttat ttttaagggt gaatattcaa tttcttttca atcaaatcaa agccaaagaa
     7741 tcttacctgt gtgttggtct tgataccgac attcataaaa ttccacaaca tttactgcag
     7801 cactccgacc caatttttga attcaacaaa cgaataatcg atgccaccaa agatctttgt
     7861 atcgcttata agccaaacat cgccttttat gagtctttgg gcgccagggg gtgggaaagt
     7921 ctgagaaaaa ccctggaata tatcccggaa gaaatttttg ttattgccga tgccaaacgt
     7981 ggtgacatcg gcaacacatc aaaactgtac gccagagctt tctttgaaaa cctcaggacc
     8041 gatgccatta cggtatctcc atacatgggt gaagattctg taaagccatt ccttgatttc
     8101 cctgaaaaat ttgtaatcct tcttggactt acttctaacc cggggagcaa tgattttcag
     8161 agaatgaaaa tggaatcagg aaacatgctt tttgaagaag ttctgatgaa aagtcagcaa
     8221 tggggaggcc ctcacaaaat catgtatgtg attggagcca cccagccaca atttatttcc
     8281 aaagtaagag aaatttgtcc ggaccatttc ctgctaattc caggcattgg cgtgcagggt
     8341 ggcagcctaa aggaaataag cgaggtaggc atgaacagta aagtaggatt aatcgttgcc
     8401 gtatcccgaa gcattattta cgcgtctgca ggaaagaact ttgcacttaa agccaggctg
     8461 gaagcaaaaa aattgcagga agaaatggct cactacctga gtttgcactt caaaaattac
     8521 aggtaagtta taatagtacc agggaacggg agaatagtaa cagtgaattc caggactgat
     8581 ctgttacttt gaggatgacc gaagagaccc tttcttatct ctggagattc caaaggttta
     8641 gccgtcaaaa cctgaaaacc tctgatggaa agcaactaac catttataac ccggggacac
     8701 ttaactcaga ctccggtcct gatttcagcg atgcaagaat tcgactcgat accatcgaat
     8761 ggtgtggaaa tatagaactt catgtgaatt catctgattg gcgaaaacac ggtcatcaaa
     8821 atgatgaagc ttacaggaat gtgattttgc atgtagtttg gaatcacgac caggaagtaa
     8881 atattggaga ttacaaaatt cccgtgctgg aactgaaagg gacggttgca gtttctctac
     8941 tctccaaagt cgaaaccctt ataaaaaata tcaccgatat tccttgcagc agtttcagga
     9001 ttgattggtc aggtgttcat cagctatcag ctctcgaaaa cgctactgta caaagattgc
     9061 aaagaaagtg cgcggaaata caactcgaat tggaatcgct taaaggggat tgggaagaag
     9121 cattttacag acgattcaca gcaaatttag gattcaaggt aaacaaagat cctttttacg
     9181 cgctggcaaa gggggtttcg caaaaattaa tcgcaaaata ttcattttct cctcctaaag
     9241 ttgagtcttt attaatggga gtggcgggtt tcttaaatca aaggattcat gatgaatact
     9301 atctgggttt aagaacggaa tttaatttcc tgcaaaagaa acacaattta tctccgattg
     9361 atccgggctg gaaatttatg cggatgcgtc ctgcaaattt tccaactgtt cgaatttctc
     9421 aattggccgc tatctatgat cacaatcaaa acctgtttcg taagctaata gagataagta
     9481 attattcaga catagtgaaa atcttaagag tggaaaccgc ggattattgg aagagtcatt
     9541 atttatttgg agaagaatcg aaatcgagga ataccgcgtt aggaaaagcc agtattgatc
     9601 tgataacaat caacacaatt gttccctttt tgttcagtta cggagtcata aatgatctta
     9661 atgaatttaa agacaaagcg ataaatttac ttcaggcaat acaacctgaa aagaatcgca
     9721 ttacaaagcg atggggaaaa gccggtttaa aattaaactc cgctttttcc actcaggggt
     9781 caatcgaatt atttgaaaat tactgccggc taaaaaaatg tcttcaatgc aaaattggag
     9841 taacctgtct ttctgaaaat caatcctaaa tggaaatcaa ttcattagtt accccaaaga
     9901 atttcttatt tactgttggt ttattaataa agttatattg cgcgctggtc ataatttatc
     9961 tgtacatttt ccactatgga atgggtgaca tgatcagtta ccaggttcaa agccataaaa
    10021 ttcttccgga attctatgcc aaccccataa cattcctgct aaaatccgac cttggatttt
    10081 cggacagtat ctattttggt cagccccggg cgatattctt tcttaagtgc atagtggttt
    10141 caagcctgat taccggtgga gatatttacc ttaccacatt cctcttttca atcttcagtt
    10201 tcattggcag ctacagagtt tttgccgcga tttatgacta ttatcccaaa gtaacatatg
    10261 aagccgccct tgcttttttg ttgctgccga cttacatctt atggacttca ggaatgttaa
    10321 aagaaagcct ggcaaatgga gcaatttgtt taatggtagc tgaggtatta catctggttc
    10381 ataaaaaaga atcccggatt ttttctttgc taatccttac catttcaggt tggctcctta
    10441 ttaaattgaa atactattat tttggagccc tgctgcccgt tttgttcgtg tactttttct
    10501 acaagagatg ggggcaatat ttaagcaata aaaaaatttc catcagaatt tcagtattct
    10561 taggattgtt gctaattgga tcattggccg ggatggtttt tcatgaatat ctcaatccac
    10621 tattgtggtt cgatatcatt tcagaaagca atcaaagtta cctggcaacg aaaagcgcgg
    10681 ggaatctttg gcagataata acacaaatac cattggcgat ttataattcc tggctggctc
    10741 ctcaactctg gcaggctaaa aatttcgttc agttcctgtc agcagcagaa tcaacttttt
    10801 ttggtttgat attagtatta tccatttaca agaatcccaa tccattcaaa tggagtctgg
    10861 aagcatgggt aatagtggct tattcattaa tccttggaat tatgctgggc ctatccataa
    10921 cgaatttcgg aagcctggag agatacagaa cttcattcta ccctttcctt tttatgctgg
    10981 tggcttccgg ccttccacaa gtcaaatcct taattaaggc tatatttgaa aaaatataac
    11041 agataaatgg ctaaaccggt tatcattttc ggagccaatg gaattggcaa ggctgctttg
    11101 gagatattta gaagttcagg gattgatgtg ttagggtttc tggatgatga tgaaaatctg
    11161 tttggcaaag aagttaacaa tataccggtg ctgggaaaga caggaaacga ggagtattta
    11221 aaattactta aagaaaagag cgaaagcttc gtggcccttg atgagaccat cctaagaaaa
    11281 aaacaaatcg aaaccctcgt taatgaatat aagtctatgc caataaatgc tatccattcc
    11341 agcgcaatca ttccggactc agttcatatt ggacatggca atttcataaa tgccggagtt
    11401 attatcggca gttacgcgaa agttcccaac cattgtctgc ttcattcggg aacaataatt
    11461 gagcacgaag cccaattggg taattttact caaattggcg caggcagcat cattaatccg
    11521 ggagtaataa ttgaagaagg agttttcata ggcagcggtg ttactatcgt ttcggggatc
    11581 atagttagaa aaaatgcgaa aataggagca ggctccgtag ttgtttcaaa tgtcgaaaaa
    11641 agccaggtaa tgtttggcaa tcctgcaaag caagtttctg cataagaaaa caggaaatca
    11701 gaaccatttt taattacatt tacaaaagtg tttcccaatg cccatttcag aaacgaattt
    11761 gatcttaaat tctgatggca gtgtttatca tttaaacctt aagcctgaag atatcgctga
    11821 cacaatcatc gcggtaggag atccttcacg tgtttacagc gtaagtaatt attttgatga
    11881 tgttgacttt gaaatgaatc gaagggaatt cattacccac accggaagat ataagggcaa
    11941 aaaagttaca gtaatttcta ccgggatcgg aacggataat attgaaattt tcttcacgga
    12001 gattgatgct cttgtgaata ttgatttgaa aacaagggag gtgaaaacga aaaaaaacag
    12061 cctgaaaata attcgggttg gtacttccgg cgcgctgcag gaagatattc ccgttgattc
    12121 gcatctggct tctgagaatg caataggaat agattctctg atgtgttttt ataatcttcc
    12181 tcaaaactcc ctggagcaaa agctggggga atcactgaaa gtaaaattat ctttacccta
    12241 cgcgccttat gttgtttccg cttcaaaaga attgtttaaa aaattcggga aagatatgct
    12301 cgtgggcaac actgtaactt gtcccggatt ctacgctccc cagggaagaa aggtccgact
    12361 tgatttgaaa attcctaacc tttttaatga gatgaattat tttcacgaaa atggtatgtg
    12421 gctaaccaat tttgaaatgg aaactgccgc ttactatgca tttggcagat taatgggcca
    12481 cgaaacattg agtttaaacg cgataattgc caacaggatt acaaataaat tctcgaagga
    12541 tccggaaaaa agtgtcgatt ccttaatccg gaaagtactg gacaggattt agttgttatt
    12601 cccgttcgtc atactctcca tagccagaat ctggctatgg gtgtaactcg gattataatt
    12661 gttataaaag ccggaaagta tcctgtgggc gatgtgagat attacaagtt cttcgtcact
    12721 tttgccttct tttcccagcc agtaaaccct cctgggatgg cgcttcatat cctccacaaa
    12781 gtccatggag cgcttgtatt ggtcttcggt gtaagtaaga cggaaagttg ttttcactgt
    12841 tttcatagtc aataaggtta aaacccacaa ttgatgctaa tacaatgctt atgccatttt
    12901 aaaatggcct gattttcaaa ttattaacga aaaacctgcg gattttgttc tgatcctggc
    12961 gttcgtacgt gggcaatttc gctcgaatct gaacaattaa ccgccttttt ttgatttttt
    13021 tgaatcctta ttccccgaac cggacggctt ctttttttcc ttcttgcttt ccggtttttt
    13081 agctgcttgt ttctttttcg atttcttctt ctttttattc tctgctggac ctccggtatc
    13141 cggatttgca ccatttggag aggttttatc agaagaagtt tctgaatctg cagaggcttt
    13201 ctcagatttt tttctgcctg aaaatgctac cgagaaagcc ttccgcaact ctaccgcagc
    13261 cttttcaaga ctatcttcaa tttcacccca tttatgcttg tgcttgtttc caaaatcctt
    13321 aagctccgtt tcaagcgtct ctttatttct ttttacttct tccattcgct tgccgaattc
    13381 tttgccggca tctttcttaa gatccgaaag ttcttctatc attccatcca gtcttttacc
    13441 taattttttg aaaagtttct cagcttttcc ttcttgttta tttttcattt aattagtatt
    13501 cgttagaatt ttcacctaaa ataaaagaaa gcatggaaag taacaatgca attgatcaac
    13561 ttcacaattt gttgaaaata aaggcggggg ttaagcagtt aagttaccgc aatgttaagc
    13621 agtcttttga aatgcttagg actgaagcta tcagcatagt ccaggagtta agcgaatcta
    13681 tcaggccgga tgacacctcc attcatgttg aatataaaga tcagggagaa ttcgaatttc
    13741 atgttaagtt ctccggtgac ttattaattt ttatgatgca caccaacatc ataacctttg
    13801 atggagaata tcaccttctt aaaagtgatt acatcagtga gaaagaaaat agaaagtatt
    13861 ttggtcatat tatgatctac aattttatgg cagattccct gaaatataac cggctcgaag
    13921 atccgggata tcttgtggca agaatgttgg taaattgtga aaatcatttc atgattgaag
    13981 gcgtaaaacc acttaatttc ttgtttccgg aaataaaagc taacaaagtg tcggctccca
    14041 tgctaagata ttttattgaa aaggctatga tagcagccat tgataatgat ctgatcgcgc
    14101 ccccttatcc aaaattgaaa accattagtc tctttgaaaa agttaatcaa aatcaggagt
    14161 taggccgcgg ccaaaaaatt ggattcaaat tgagcatgag ttccgattaa cactccacat
    14221 caacaaactc aaattttata ccatcaaaca agccttggtc actgagtttt agttccggta
    14281 ttaccaataa agccataaaa gaaagagtca taaacggtgc agataaccgg gagcccattt
    14341 gctttgcttc cacatctaat gcggaatatt ttttggccac ttcataacca tcaaggtctg
    14401 acataaggcc tgcaataggc agaggcagga tactggaatc aactccattt atggctgcaa
    14461 ttccaccctt ttcttcaata accagattaa taacttttgc aatgtcatta tcattggtcc
    14521 caaccgcgat tatattatga gagtcgtgag caacagaact tgcaatcgca cccctggaca
    14581 acccaaagtt ttttataaag ccaattgcgg gtctcgaatt ttcataccga tttacaacaa
    14641 caagtttcag aatgtctctt gaaacatcgg gtacaatgtt cccgtcaatt tttgttgcct
    14701 gctcaattcc ggaggttgtt acaagctcgc catcatgcgc ttcaattacc ctgatttggt
    14761 tcacgctaga ttttaatgca aggctcccgg catcaattgg tgatgtattg aaattattaa
    14821 ctataggtga aagtaccctt gacagctttg tagtcccgtt ctcagccaca tttattccgt
    14881 tgatgtatgt gcgcaaaaca tttactcgtt taaaattatc aacgataata aaatctgccg
    14941 gatcgccggg tcttaatctt cccaccccca atccataatg tgtgattgga ttgaggcagg
    15001 ctgcctgaag aactttgaaa agatcaactc cctttgccaa tgctctttcc acaagtttat
    15061 taatgtggcc ctcaaccagg ctatcaggat gtttatcgtc ggaacaaaac atcatatttt
    15121 catagtgttc gtgaaggaga tcggccaggg catcaaaatt ccttgcagcg ctgccttccc
    15181 tgatgataac cttcataccg aaacttaatt tatccagggc ctcctctttt gtgaaacact
    15241 catggtctgt agttatgcct gcatcaatat attttttagc ttgacctccc ttaagtcccg
    15301 gggcatgacc atccacttgc ttattcattt tgtgagccag ggagattttc gccaaaactt
    15361 ccggatcatt atttaatact cccggccaat tcatcatttc cgcaagatat ttcacttcat
    15421 ttaactgaaa cagctcctct atttcattaa cagagattgt ggaacccgcg gtttcaaatg
    15481 gagtggccgg tacgcaagaa ggggcgccaa agtagaattt gaaattcgtt ttctttccat
    15541 cctcaatcat atacattaca ccggccttac ccaaaacatt gccaatttca tgagggtctg
    15601 aaacggtact caccgttcca tgtacaacgg caagtctggc aaactcagaa ggagtaagca
    15661 tgctgctttc aatatggaca tgagcgtcaa taaagcccgg caagataaat tgttccggaa
    15721 catccgcatt aatttcttca acagaaatta tttttccttc tgaaagacgg acaacacctt
    15781 taaatatttt cttaccttca atgtcaatta ttctgccttt gatttccatc ttttgggtgt
    15841 gcaatcaatt ttggtaaaat aataaacttt tacagcaatt cactaaatga aaaacataat
    15901 tgtagcgatc gatggtcatt cggggtgtgg aaagagttcc accgcgaaag cggttgcccg
    15961 gagactgaac ttcacctaca tagactccgg agccatgtat agagccgcag ctctatattt
    16021 aaaaagattg aatattaatc cggacgagag ggaaaaaatc aaggaaagta tctcagaaat
    16081 aaggatagat tttgaaaaag atgccaatag caattttact acaattctaa atggcgaaaa
    16141 tgttgaagaa gaaatccgaa gcatggagat aaacaggctg gtaagccggt atagtgtggt
    16201 acctcaactc agggaggcca tggtttcgaa ccaaaggatg ctaagtaaga accgaaacgt
    16261 gattatggac ggtcgcgata tcggtaccat tgtttttccg aatgctgatt taaaattttt
    16321 tatgactgct gatttgaagg tcagggcact cagacgccaa aatgaactgg cagcccaggg
    16381 taagatagaa aatttggatg tggtaatgca aaacctggca cacagagatg aaattgattc
    16441 tacacgggaa tcaagcccgc tcattcaggc ggatgatgca atggttattg acactaccaa
    16501 tttaactatg gaacatcaaa tagaaatggt agtgaatttg gcagcagaaa gaatgaagga
    16561 gtagaagcaa taaattagta aaattttaag gccaaagttc atttcaacaa attaaagtct
    16621 ataatttcta ataaagatta gttcggtttt aattgaataa aattttgtta attttgccct
    16681 cgttcagtag gtttaaaaca cgactctgaa ccctaactat gtcccaattt atcaagctga
    16741 aaagaggatt tgatattaat ctagctggta aagcctcgaa taaggtagaa aatccaaccc
    16801 aacctgaaaa atttgccata aagcccttcg attttatggg actaaagcgg ccgaaattac
    16861 ttgttgaaat tggagataga gtgctggctg gtcaaccgct gatgtatgat aaaatgtttg
    16921 aaggagtaaa ctacgtctcc ccggttagtg gtgaggtatt ggatattgtg cggggcaaaa
    16981 aaagagtact gcttgaaatc gtaataaaat ctgacaatga atttaaatgc ctgcagttta
    17041 aaaaatattc agtgtctgat attaacaatc tggaccgcca aacaataatc gaagccatga
    17101 gcaaaaccgg cgtttggcct aatatgattc agcggccatt cggaattgtt gcagatccaa
    17161 aaactgaacc taaatccatt ttcatttccg ggtttgacag ccatcccctt gcacctgatt
    17221 atggtgtatt gttaaaagac catcaaaaag aattacaagc gggaatcaat gtgctaagga
    17281 aactaacatc cggattaatt catttcaatg taaatggttc tggtgaaatg ccaaccgttt
    17341 attccgcctt gaacaatatc cagctcaaca aattcaccgg cccgcatccg gtaggaaacg
    17401 ttggtgttca gatacaccat attgatccaa tcagtaaagg agatattgtt tggactgtaa
    17461 atcctgttgg cgtggttcag attgggactc tgttcctgga aggaatttat gatgcttcaa
    17521 aattggtcgc atttgttggg tctgaagtag cagaaccgaa atattacaaa acttattccg
    17581 gagcttgcat tgatacaatt gctcgggaaa atatcaggaa tagtcatgtc agatttgtaa
    17641 gcggcaatgc tttaaccgga aaaagaattg agcctgccgg attcctggga ttttacgatc
    17701 atatgataac ggttttgccc gaaggcgata aagctgaatt tctcggatgg ataactccca
    17761 cttttcagaa gttgagcttc caacgtgcgt tcggattgtt atccttttta aattcgaaaa
    17821 aggaattcgc gttatcaaca aatgccaggg gcgaaccaag agccttcgtg caaactggtg
    17881 tttttgaaaa agtaactcca atggatatat ttccattgta cctggtaaag gcaataatgg
    17941 cggaagattt tgatgacatg gaagctctgg gtatttacga agtaattgag gaggacctgg
    18001 cgctttgtga attcgtggat gtttcaaaac atgacatcca ggatattctc aggcaaggaa
    18061 tagaattaat tcaaaccagc tagtaaatga aatttttgaa aaaccttctc gacaagcaac
    18121 ggcccctttt tgaaaagggc ggcaagcttg aaagatttta tttactcttt gaagccgggg
    18181 aaacttttct tttttcacct tccgaaacta caaagcccag gggcgtgcaa atacgcgatg
    18241 ccattgactt aaaacgactg atgatgactg tcatcatagc catgcttccc tgcttattat
    18301 ttggtatatg gaatgttggt caccaacatt ttattgcagt aggtgaactt aatgcttcgg
    18361 cggcagataa gtggttgatt gggctaagag tagtaatacc cattttgctt gtatcctata
    18421 tagcgggagg tttggtcgaa gttgtgtttg tggtgataag aaagcatccc ttaaacgaag
    18481 gctacctggt taccggaatg ttgatcccat tggtagttcc acctacaata cccctttggc
    18541 aggttgcgtt ggcggcaatt tttgccgtcc tgattgctaa ggaagctttt ggaggaaccg
    18601 ggatgaatgt attgaatgtg gccctgaccg caagagcttt tctgtatttt gcctatcctt
    18661 ctcaaatttc aggagacgtt tggacctacc ttggtgataa aactcctgtt gacggatatt
    18721 caggtgctac acccctggct ctgactaacc aggcaataac aaccggtgaa agtgtagtca
    18781 catctctgaa taattttgga gcaggctggc atgaaggagt ttacagattt tcgagcatgt
    18841 ttttcggatt tttgccaggt tcaattggtg aaacttcaac gttgatgtgc ttgattggcg
    18901 cggcaatact tataacgaca ggcgttggaa gctggaaggt aatgttgggt gtgattatag
    18961 gagcatttgc catgggaaat tttgtgaatt tgcttgctgt taatgcctac atggagatgc
    19021 ccgcatatta tcacctggta attggaggac ttgcgtttgg aactgttttt atggctactg
    19081 atcctgttac tgcagctcaa acggaacgcg gtaaatggat atatggcata tttatcggta
    19141 tattaacagt aattatacgg gtatttaacc cagcataccc tgaaggtatt atgctggcca
    19201 ttttattgat gaatgttttt gcgccactca tcgatcatta tgttgtacag ggaaataaga
    19261 aaaggaggtt aaaacgtgcg acgatctaac ggatatatca taatcttcac cgcgatattg
    19321 actattgtag ttggcggatt gctgtcattg acttctctgg ttcttgggcc tgcccagaag
    19381 aaatcaatcg aactggatac aaaatcacaa atcctaagcg ctgtgatgca aatacaaaaa
    19441 ggagacgata ttctggatat ctaccggaaa agaattcaat ctgttgtagt cgatgcccgg
    19501 ggcaatgaga ttactacaga cgaaaaaggc aaaccaattc aggctgaaaa tatagatatt
    19561 gcaaaaaact tcaagaaaac cagggaaaac agacagtatc cggttttcaa attcatgaat
    19621 aaagaaaatg aattgcaggt ggatgcatat attttgccca tttatgggaa tggtctctgg
    19681 gataaagttt ggggctttgt tgccgttgac gaaaaatttg aatctattgt gggagtttct
    19741 tttgcccaca aggcagaaac tcccggcttg ggtgcaagaa ttgctaccac tgaaattcaa
    19801 accaggtttg agggcaagaa gattcttaat gaagccggtg aagtggttag cgtgacaatg
    19861 cagaaaggag aaaaaggagg aggacaggca agcattgatg cctttgcagg aaagccgcac
    19921 caggttgacg gaatgtcggg tgccacactt acagccaaag gtgtcaataa tatgatgctg
    19981 gattacttaa cttcatataa accatatttc gagaaagtta aaagccaggt ttctctgtaa
    20041 agcttttact tgtataactt tgttaaccag ataaacggag aagaatttta tgagtacaga
    20101 aacagttgag caagtacagg aagtattagt taagaaagta gaaccattat tttcgaagcg
    20161 cagaaagaaa tttgtaactg atccattgaa tgaagataat ccaatcaccg ttcaggtatt
    20221 gggaatttgc tcggcattag caataactat taagatggag ccaacgctca tcatgtcgat
    20281 agcggtggtg ttcgtgattg tattttctaa cctgatcatt tccgtcatgc gaaatctaat
    20341 ccctaaccgg gttagaataa ttgtacaact ggttgtagtt gcaacgctgg taacggtggt
    20401 aagtgaaatt ctaaaagcct attactttga aatgtacaaa gtgcttggag cttttgtcgg
    20461 gttaattatt acgaactgta tagttatggg aagacttgaa gcttttgcaa tgggcaataa
    20521 accctgggat agtgtccttg acggaatggg aagcggattc ggatatgcct ggattatcct
    20581 cactgtcgct tttttcagag aactttttgg ttccgcatca ttatttgact tcaaaatatt
    20641 ttcagccata ggactttccg gttttcctac caacgcaatg atggtagatt caattggggc
    20701 ttttatgatc cttggattac tcatttgggc ccaaagaagt aaaacagggt atgttgaaca
    20761 ctaaaaaaat taccgtagat ggagctgatt aacttaggga ttaaggccat ttttattgag
    20821 aacatgatct ttgcctattt cttgggaatg tgttcatttt tagctgtttc caaaaaagta
    20881 aacactgcca ttggcttggg tgcggcggta atatttgtgc ttactctaac ggtacctctt
    20941 gattggctta ttcaggaata tattctgaga gagggcgctc tggcgtggat cggcccacaa
    21001 tttgaagaaa taagccttga atttctccag tttataatgt tcatagctat tatcgcggct
    21061 atagtacagt tagttgagat gattattgaa aaaatttcac cagcacttta cagcacattg
    21121 ggtattttcc tcccattgat cgcggttaat tgtgcgatcc ttggatcttc tctttttatg
    21181 gtccaaagag attacacgct tgctgaggca acggtatttg gttttggctc aggagtggga
    21241 tggtttttgg ctatcgtggc tttggctgca atccgagaaa agcttaagta ctctaacgtg
    21301 cctgacggat tgaaaggact cgggattacc atgctcatca ccggcctcat ggggattgca
    21361 ttcaagtcct ttatagggat tgctctttaa ttattgatcc tattccaagt tctaatcgta
    21421 ttcttttttt cgggtgataa ttattacgcc cggggaatac gcaacccttt tgaatacttt
    21481 tcattattga agatggccgt gccaatactt catggatgac aaagagattt atgaccgaat
    21541 aaaatcggat aagcaaaact ttgcaatatt gtatgatcaa tattttgttc cgattaaaaa
    21601 gtatattctc aggcgtatag gaaactacga tatctcctgt gacgttaccg cggaaacgtt
    21661 cctgaaggct taccttaaaa taagccattt tgaataccgc gggatctcat ataaagtatg
    21721 gttgtaccgg atagcaacca atgaagtaaa tttatttttc aggtcaaaaa aatataaacc
    21781 actaaataaa attaatcttg aagatttcaa caatgaaact ttaattactt cccaacaaga
    21841 agataaggat atttgggaaa aggagttaca ggatcaccat gacttcctta aggcccagca
    21901 agtcataaaa aatttaccaa taaagtatgc cgaggtgctt tcactaagat attttgaaaa
    21961 agcatcaata aaagaaatcg cggaaatcct tgataaacct aaaggaacta ttaaatctct
    22021 gtgtacgcgg ggattgcaaa aagtgaagga gaaaatgcaa cccggaactg aatcttgaat
    22081 tattgaaaga aatagttaga tatgaaaaac atggaagatc tgccggttcc tgatcctgaa
    22141 ttaagtccgg atcagaatct taaaaagaca atattgaata cccaaacttc agccagaata
    22201 ggaattcctc tccttatttt gcccggtatc ttgctcatca gcatgatctt gaaagaagag
    22261 ctgggtatta acctgggaat aattaatgct attacagaac tcattggcaa catggataaa
    22321 tcgccctaca ccggctggct aagtcccttg atattccttc tattgccgtt gatggcaatc
    22381 attatttttt tactttcaat cactcacttt tcttataata aagaaaatta tattctccaa
    22441 atcaatatta agttcaagac atttaatatt atgctgttac tgattgccgg tgccatactt
    22501 ggcacaataa ctttgtacct gattcttgaa aatttgaatc atttcatgct cgataaattt
    22561 ggccagcctc tatagcggcc attatttgcc gtccatattc aatcgttcgc caattacatc
    22621 aaattgaagc ttttagtttg ccaattcgat cagaaaaatc taatttagtt tcccttcaaa
    22681 tcattcataa aaattatgag gaaaatactt caacttgttg caattgcttt ggtggcattt
    22741 gaatgtaccc agcccgctga aaaagcaatt gatgattcaa tatttacacc aatgattggc
    22801 gattactacc agggtctgtt ggaattgtat ctttgaatct caaattaaca gtggatagcc
    22861 tcgaaggtta acaaaatggg caggaagatt agtttcaaag tcagtgacaa atcaggcagt
    22921 gtgactggaa tttacgaaaa gtccgggaaa ggagatcgat tgctggtgtt gggtcatggc
    22981 gccggtgcac ccatgcacag taccggaatg caattgattg cggaaacact tgcaagtaag
    23041 ggcattagta cgctgcgata caattttccc tatatggaaa aaggcataaa gcggccggat
    23101 gttccggcgg tggcccaact tacagtaagg aaggccgttg aaaaggcaaa gtccctggag
    23161 ccgggagcga agatttatgc aggtggaaaa tcttttggag gaagaatgac ttcacaagct
    23221 gaatctgaag agttgttggg agtggaaggc ctggttttct atggatttcc attacacgca
    23281 cctggaagag atggagtgga aagagctgat catctgaacc aagtaaaaat acccatgctt
    23341 tttctgcagg gaacccggga tgctttggcc aacattgctt tgatgaagaa agtctgcaaa
    23401 gagctgaaga aatcaaaatt agaaataatt gatgggggag accattcatt caaagtacct
    23461 aagactgatc ctaacgctgt ttttgaaaac cttgcctccc ttgttgctaa ttggatgaag
    23521 tgaatctaac cggttgtgtc caggccattt cctgttcctt ttcataaacc gggttctcca
    23581 tgagcttgga agaagtgatc cgggctcgta catattcaac atcttcggtc aattgatact
    23641 ggacctcagt tgcagactgt cggtgcaaaa tttttggact ttcacctgca aattgaccga
    23701 taaattcaaa ttcataagta acatcggatt ccggcctgat ggcaatggaa agaagattat
    23761 cttcaaaggt gagttttttc agaataacgc ccgtggttga ataaaattgg cccgcttcca
    23821 tggcattgat caatgtttcg ggagttagct cgtcgcaatt caccatgacc cagccccggc
    23881 ccgcattgct gtaagaactt ccgaacaagt gataattgtg ggcatcatca gtggccaggc
    23941 caaagaggag agcatcgccg gattttatcc tggcggaatt gataagatcc cacatttgct
    24001 cggtgctgat atgagcagaa tctccccggt tattcacggc ggcatggcca ttatacactt
    24061 caaaaaacct ttcacctctt acaggaatta aatcctcaac ggtaattgca tagccaaagt
    24121 tgggatgatt gacatgaacc ataattggaa ctcccaacct ggcgcgttgt tcaattaccg
    24181 tattaatatt attctgcaaa acctctgtca cagaatttcc cccttgcggt ggaattaatt
    24241 cctgaaggtt atttgcattc aggtgcagcg gtttaccgtc aaatctgtcc gtaatttctt
    24301 cggcttgcaa aatcagaaat tctccactcg cgtcaaaaag cttgcgatac tcgttgagtg
    24361 tttttagttt cacgctgatt ctaccggtgt cttccttgaa attcacccac tcttccccat
    24421 acttttccag atatttttga aatgcttcct catgcacgcg gctttttgga atcaatttcc
    24481 atttttcacc ttcagcaata acattatgtt cactaaggga aacaaaatca tacccgttgc
    24541 tcttatacca gtccatcacc atttccgggt aatcatcgcc gtcgctccaa taggaatgcg
    24601 tgtgaagatt tcctttaaac caattttgct gtgagacttg gtgttttgga atacagaaag
    24661 ttaaagttag acagagcaga aggcctattt tggttttcat ggatgctaaa ctaccctttt
    24721 gcggtgtttt tcgcacattg gaagttgaaa aaaatatatt ttccttttct cccgcagatt
    24781 acgagggatt tacgtagaaa actcctgaaa acaattctgc ggtgatttgg gatatcagcg
    24841 ggattctata aaattttctc ccgcagatta cgcggattta cgcagaaaac tcctgaaatc
    24901 aattctgcgg tgatctgcga tatcagcggg attataaaat tttctcctgc ggattatgcg
    24961 gatttgtgcg gaaaactccg gaaataaatt ctgtggtgat ttgcgatatc agcgggattc
    25021 tataaaattc ttttctcccg cagattatgc ggatttgcgc agaaaactcc tgaaatcaat
    25081 tctgcgattt ctatgtttta tgcaagggca tgtttgtaaa tcttttgata ttttctgttg
    25141 ttttttatgc aggcaaaaac acgacttatc aacttattac gcacagcgtt gataacactc
    25201 attttgtttt ttcc
//
LOCUS       DASWHX010000092         6732 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_3039430, whole genome shotgun sequence.
ACCESSION   DASWHX010000092 DASWHX010000000
VERSION     DASWHX010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 6732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6732
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3039430"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(135..554)
                     /locus_tag="VGA21_06275"
     CDS             complement(135..554)
                     /locus_tag="VGA21_06275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650146.1"
                     /translation="MKEAMLVIHFIGLTMALGAGFSNMFLGAAAAKLEPEERGKFMSK
                     TFVLVRMGQTGLGLLLISGFYLITPYWKVLGEMPFLTAKLVLVGILIIMVSIVSVMAR
                     RALKENDPARLMKVRPYGMLNFFIGIAIVIMAVLAFN"
     gene            complement(687..2450)
                     /locus_tag="VGA21_06280"
     CDS             complement(687..2450)
                     /locus_tag="VGA21_06280"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX9650147.1"
                     /translation="MNSATLKVFAFTLLISSCTRTQQSIRILEGSDLEVGIEIQSELT
                     AQSADTFRLNLPARTFISGNAEQITVDVVVNILDSAKKSVSGFDDAARGPEPFILTAR
                     KAGIYYIVISPFQQQEGKYSLKIGSAEPLAMDPAGRVDQIARSLTDENGPGFSIAIAR
                     DGETIYKNSFGLANLEYSIPNTQQTIFHIASVSKQFTAFSIAMLVSQGKLSLDDDIRK
                     YLPEIHDFGTPITINHLIHHTSGLRDQWNLLMIAGWRLDDVIRMDHIIKMMARQRELN
                     FHPGAEFLYCNTGFTFLAEIVGRVTGTPFPEWTKQNIFEPLGMNRTLFYDDHEKVVEN
                     RAYSYYKPDSVYKKRVLSYANFGATSLFTTVEDIMKWAANFETMTVGNPKVMAMMEQK
                     FVLNNGDTIDYGFGQGIGEYKGLKTVSHGGADAGYRTTLIRFPEERVSIAVFSNLGSF
                     SPNGFAYAAADEFLIDQLVEEPEDEDSENPGAPQASEDLFDRNTVKLADYAGRFYSEE
                     IQSFYDIRVENDTVLMAHHQRNPDIVLNAVAPDEFRGNMGWMGNVKFTRNGKGKVTEM
                     LISNGRVRNLSFKREGGVIGI"
     gene            complement(2647..3528)
                     /locus_tag="VGA21_06285"
     CDS             complement(2647..3528)
                     /locus_tag="VGA21_06285"
                     /inference="COORDINATES: protein motif:HMM:NF039197.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5694 domain-containing protein"
                     /protein_id="HEX9650148.1"
                     /translation="MKRLILIICLIPIICKAQPGHAPEKLLLEIPKVKVMVLGTFHFD
                     DSGLDDHKNEKPLAMMSAEKQKEIQEIVDQLIRFKPDKIALEFKTLLQPRIDSLYNDY
                     GSGKFQLGKNEIYQLGFRVGEKLGHDKVYGVDANPRWYDFILNMEEQEYENYKNTLIS
                     KLANEKPHTRELYPLYQQYYKIKDSLLHVVPLRDYFLKVNSEESIKANHGIYLVDSFK
                     LGIGSDDEDERYFGADMRTAWYNRNLRIFQNIQRILEAGDERVLLIIGYGHLPIIKHC
                     IEYSPEFEWVELSEYLK"
     gene            complement(3531..4334)
                     /locus_tag="VGA21_06290"
     CDS             complement(3531..4334)
                     /locus_tag="VGA21_06290"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HEX9650149.1"
                     /translation="MKGCVLLFFLLIQLEIVKAQSDPDYTIELSNPGEKSSLYLKSYN
                     ASEVMLTGYDGRNIQVTFGSPGQGKFSKEDFLFDMYEGNNKVSIINRSSANDQQLIIS
                     LKIPFDCEVIVDSYFAKKINAANIQSNVKISSQTADILAANIRGNIEAKTFNGKLNIE
                     KLDGAAILNNTEGDIIIKFSSLSTSDPSAITNIHGNIEIHLPESEKATLELNSLKGKI
                     TTDLKLTMANRAAGDSNAKKYSVLRQLNGGGVWFSIENLHGNISLLKTK"
     gene            complement(4339..5088)
                     /locus_tag="VGA21_06295"
     CDS             complement(4339..5088)
                     /locus_tag="VGA21_06295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650150.1"
                     /translation="METRLSELKQKYFDGKTSLEEEVELKSLLEKSAESEDKELLMQF
                     NYFEEIKNYSPGGEFGAGIQRQIRKVYIKNLRDWTFRIAAGVILIAVAFYGGLNYGRS
                     NSKNDQFLSMQNDIRKLYQISTEVLLRQESAHDRLKAMVLVNSIALKPDSITLDLIFR
                     AYTSDDNISIRLSAFELLSNYASDNYIRESLINSITHQDSEIIRYKLIHLALQLKEKS
                     AVPLLERIMTEENISSEMKSEIDMAIKQINS"
     gene            complement(5069..5722)
                     /locus_tag="VGA21_06300"
     CDS             complement(5069..5722)
                     /locus_tag="VGA21_06300"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX9650151.1"
                     /translation="MGANNKLHYSFVSGIGHRLIFAGVRNYGRFKVLIISCQELIQIP
                     SFYKGMNFKEFTGSIDVVREKLLRYALWVTGTRGEAEDIVQETFIRMWKMKKKLHEYD
                     NLENLGKRIIKNLSIDIFRSRRKFIDIDSAAFNHIKSPEEEMINNENFTKLKNIIRNL
                     GEPYHTVLYLRAIEGRDIKEISKIINAKHNTVEVILSRARKKIREEYKMMDYGNKIK"
     gene            complement(5677..>6732)
                     /locus_tag="VGA21_06305"
     CDS             complement(5677..>6732)
                     /locus_tag="VGA21_06305"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX9650152.1"
                     /translation="VLLGINACSEDNEVVLETPLQKSDGIITGSAKEQGFNVKLLSTM
                     IDELNGGNYSVNSVLIAKNNYLVLEEYFQESAQGITAIQSVTKSITSALAGIAVDQYN
                     LLADTPVINFFPELANLDWGDEKESITVQNLLTMSSGLQWNESALPYNDPLNDHNLLN
                     QSDDWIEFILSRPMTSSPGSVFNYNSGLSIVLGEIIKRQTNQSIGQLSISKLFSKMDT
                     EFVSWFTDDSGIFQTGGGLSFLPRDMLKFGLLYQNNGVWNGEQLLSTGWVQQSTTQQG
                     PNAEYAYHWWLTSFTVNGQVYGSFYAWGNGGQFIVVINELSLVIVFTASNFENNLQAR
                     ENSWELITNYIIPSSVE"
BASE COUNT         1864 a         1519 c         1207 g         2142 t
ORIGIN      
        1 atcgttgaaa cggaatatgc cccaattggg ctgctttgaa tatgatttgt tatataattt
       61 atattatgtt aaataggata acccaatcat aactattctc tctaccttac ttattcagga
      121 agtctttctc tcccctaatt aaaggcaagc accgccatta taactatagc aatacctatg
      181 aagaagttta gcatcccgta gggcctgact ttcataagcc tggcgggatc attttctttg
      241 agggccctgc gtgccattac cgatactatg gaaaccatta tgatcaggat gccgaccaaa
      301 actagcttgg ctgtcagaaa tggcatctcg ccaagcacct tccaatatgg tgtaatcaga
      361 tagaatcctg aaatcagcag caatcccaga cctgtttgcc ccattcgcac aagtacaaaa
      421 gtttttgaca taaactttcc tctttcttcg ggctcgagct tggctgcagc cgctccaagg
      481 aacatattgg agaaacctgc tccaagggcc atagtcaatc caataaagtg aattacaagc
      541 attgcttcct tcatacattt ggattttggt tatttaatat aagactttaa atatggtttg
      601 ctgattcttt aatttcaggt ttcctaaaaa ctagcacttt ttacgtaacg tattttacgt
      661 aatgaacatt cacctcttaa ttagtattat atgccaatta ctcctccttc ccgcttaaag
      721 ctcaaattcc tcacccttcc attgctaatc agcatttcag ttactttacc cttgccgttg
      781 cgggtgaatt tcacatttcc catccacccc atattgcctc gaaactcgtc cggggcaacg
      841 gcgttcaata ctatatccgg gttgcgctgg tgatgggcca ttaatacggt gtcgttctcc
      901 acccttatat catagaaaga ttggatttct tcactgtaaa accgacctgc atagtctgca
      961 agcttcactg tgttacggtc aaacaggtct tcacttgcct gcggcgcacc tggattctca
     1021 gagtcctcat cctccggctc ctcaacaagc tggtcaataa gaaattcatc agccgccgca
     1081 taagcaaaac catttggact gaaggatcct aaattgctga aaactgcaat agaaaccctc
     1141 tcttccggaa atctaatcaa agttgtgcgg tagcccgcat cggcaccacc atgcgaaacc
     1201 gtctttagcc ctttgtattc cccaattccc tgaccaaagc cataatcaat ggtatcgccg
     1261 ttgttcagta caaacttctg ttccatcatc gccatcacct tgggatttcc aacggtcatg
     1321 gtctcgaaat tagcagccca tttcatgata tcttcaacgg tggtaaacag gcttgttgca
     1381 ccaaagttgg catagctcaa aacccgcttc ttataaactg aatccggttt atagtatgaa
     1441 tatgcccggt tttccaccac tttctcatga tcatcataga ataacgtgcg gttcattccc
     1501 agtggctcaa aaatgttttg tttagtccat tctggaaatg gtgtaccggt aacgcggcct
     1561 acgatttcag ccaaaaaagt aaaaccggtg ttacaataca gaaactccgc cccgggatga
     1621 aaattcaatt cccgctgcct ggccatcatc tttataatat gatccatcct gattacatcg
     1681 tccagcctcc atcctgctat catcagcaag ttccattgat cccgtaaccc gctggtatga
     1741 tgtatcaagt gattgatcgt aatcggggtt ccaaagtcat gaatctcagg aagatatttt
     1801 ctaatatcat catcaaggga aagtttaccc tggctcacaa gcatggcaat cgaaaaggca
     1861 gtaaactgtt ttgaaaccga ggcaatatga aatatggttt gttgtgtatt tgggatgctg
     1921 tattcgaggt tggccaatcc aaaactattt ttataaatag tttcaccgtc cctggctatc
     1981 gcaatggaga atccgggacc attttcatca gtcaatgagc gtgcaatctg gtctaccctg
     2041 ccggcaggat ccattgccaa cggttcagcg cttccaatct tcaacgaata ctttccctcc
     2101 tgctgttgga acggtgaaat cacaatataa taaatccctg ctttcctggc cgttaaaata
     2161 aagggttcgg gtccccgggc cgcgtcatca aatccactca ctgatttctt agcggaatca
     2221 agaatattca ccactacatc cacagttatc tgttccgcat ttccgctgat aaatgtcctg
     2281 gcaggcaggt tcaatctgaa agtatccgcg ctctgagccg tcagttccga ttgaatttct
     2341 atacccactt ccaggtccga gccttcaagg attcgaatgg attgctgcgt ccttgtgcag
     2401 gagctgatta ataaagtgaa cgcaaaaacc tttaatgtag cgctattcat aattttgcag
     2461 gtggcttaat aagccggtaa tttattgatt aataaatcac ttggatgtat caaaatgaac
     2521 aatttgttta ttcagcctcc aggttttccg caggaatccc gttgcttgaa ctaagcggag
     2581 tttcattaaa gtactttacg taattatccg gggagcgcaa aataaaaaat cacaattgta
     2641 tcgcaatcac ttcagatact ccgataattc cacccattca aattccgggg agtattcaat
     2701 gcaatgctta attatgggga gatgaccgta accgataata agcaataccc gctcatcccc
     2761 ggcttcaagt atccgctgga tattctgaaa aatcctcagg tttctgttgt accatgcggt
     2821 cctcatatcc gcaccaaaat atctttcatc ctcatcatcg ctgccgatac ccaacttgaa
     2881 tgaatcaacc aggtagatac catgattggc tttgatgctt tcttctgaat taacctttag
     2941 aaaataatct ctaagcggaa caacatgtaa caatgagtct ttaattttat aatattgttg
     3001 atataatgga tacaattctc ttgtatgcgg tttttcattt gccagttttg aaatcaatgt
     3061 attcttatag ttctcatatt cctgttcttc catgttcaaa ataaaatcat accacctggg
     3121 atttgcatca acaccgtaca ctttgtcatg cccgagcttc tcccctaccc ggaaccctaa
     3181 ctggtaaatt tcatttttgc ctaattgaaa tttaccggac ccataatcat tatacagtga
     3241 atctattcgc ggctggagca aagttttgaa ttccagcgca attttatccg gcttaaatcg
     3301 aattaattga tccactattt cctgaatctc tttctgcttc tccgctgaca tcatagctaa
     3361 aggtttttca tttttatggt cgtcaagacc ggaatcatca aaatgaaatg ttccgagaac
     3421 catcaccttt acttttggaa tttcaagcaa caatttttcg ggagcatgcc caggctgagc
     3481 tttgcagatt atcggaatca ggcagatgat tagaataagt cgtttcatag ttatttggtt
     3541 tttaataatg agatgtttcc atgcagattc tcaatgctga accagactcc cccaccgttt
     3601 aattgtctta gaacgctata cttttttgca ttactgtcac cggcagctct gtttgccatc
     3661 gttaatttga ggtcggtggt tatcttccct ttcagggaat tgagttcaag cgtcgctttt
     3721 tctgattccg gtaaatgaat ttcaatattg ccatgaatat tggttatagc cgaaggatct
     3781 gatgttgaca gactggaaaa ttttattatt atgtcgcctt ccgtattgtt tagaatcgcg
     3841 gcaccatcaa gtttttcaat attcaactta ccattgaaag tttttgcttc gatatttccc
     3901 cggatgttcg cggccaatat gtccgctgtc tggctgctga tttttacatt agattgaata
     3961 ttagctgcat tgattttttt tgcgaaatag gaatctacaa ttacctcaca atcgaaaggt
     4021 atttttaaag aaatgataag ttgctgatcg ttggcggagc ttctgtttat aattgaaact
     4081 ttgttgttcc cttcatacat atcaaaaaga aaatcttcct tactgaattt tccctggccc
     4141 gggcttccga aagtaacctg gatgttccgg ccatcataac cggtaagcat aacttctgaa
     4201 gcgttatagg atttcaaata caatgaactc ttttctcccg ggtttgaaag ctcaattgtg
     4261 taatccggat cgctctgggc ttttactatt tcaagctgaa tcaacaagaa aaataatagt
     4321 acgcatccct tcatcctatt atgaatttat ttgtttaatc gccatatcaa tttctgattt
     4381 catttcgctg gaaatgttct cttccgtcat tattctttcc aaaagaggta ccgccgattt
     4441 ctcttttaat tgcaatgcaa ggtgtatcag cttatagcgt atgatttctg aatcctggtg
     4501 cgtaattgaa tttatcagac tttcccggat atagttatcc gacgcataat tactgaggag
     4561 ttcaaaagct gaaagccgga tacttatatt gtcatcactt gtataagcac gaaaaatcaa
     4621 atcaagggta attgaatcgg gttttaacgc gatagagttg acaagtacca ttgcttttaa
     4681 ccggtcatgc gcggactctt gccgaagtaa aacttcagtt gaaatttggt acaattttct
     4741 gatgtcattt tgcatgctca gaaactgatc attctttgag tttgaccggc catagttaag
     4801 tccgccgtag aaagcaactg ctatcaggat tactccggcg gcaattctga aagtccagtc
     4861 gcgaaggttt tttatgtaca cctttctgat ttgtctttgt attccagcac caaattcacc
     4921 accggggctg taatttttaa tttcttcaaa ataattaaac tgcattagca actccttatc
     4981 ttcactttcc gcggattttt caagcaatga ctttaattca acctcctctt caagggatgt
     5041 ctttccatca aaatattttt gtttgagttc acttaatctt gtttccataa tccatcattt
     5101 tatattcttc tcttattttc tttcttgctc ttgaaagaat cacctcgaca gtgttatgct
     5161 ttgcatttat gattttagaa atttccttta tgtccctccc ctcaatcgct cgcaaataca
     5221 aaactgtatg gtagggttct cccaggtttc tgattatatt ttttagtttt gtgaaatttt
     5281 cattgttgat catttcttct tccggacttt tgatgtggtt aaacgcggca gaatctatat
     5341 caataaattt tctccggctc ctgaatatat caatcgaaag atttttgatt atccttttac
     5401 cgagattttc cagattatca tattcatgca acttcttttt catcttccac attcgaataa
     5461 atgtctcctg cacaatgtcc tccgcttcac cgcgcgtgcc tgttacccat aaggcatatc
     5521 taagcagttt ttcccttacg acgtcgatac tacctgtgaa ttctttgaaa ttcatacctt
     5581 tataaaaaga cggaatttga atcaattcct gacaagaaat gattaaaacc ttgaacctgc
     5641 cataattccg gactccggca aagattaacc tatgccctat tccactgacg aaggaataat
     5701 gtaatttgtt attagctccc atgaattttc acgagcctgc aaattgtttt caaaattgct
     5761 cgcggtaaat actattacta aagacaattc attgataaca acgataaatt gtccgccgtt
     5821 gccccaggca taaaacgatc cgtaaacctg cccattaacc gtaaacgagg taagccacca
     5881 atgatatgca tattccgcat ttggcccttg ctgagttgtc gattgctgaa cccaaccggt
     5941 gctcaaaagt tgttcaccat tccaaactcc attattttga tacaggagac caaacttcag
     6001 catgtcgcgt ggaagaaaag acaacccccc gcccgtttga aatatgccgg aatcatcagt
     6061 aaaccaggat acaaattctg tatccatttt ggaaaataat ttggatatac ttaactgacc
     6121 aatggattgg tttgtctgac gcttaataat ttcaccaaga acaatggaga ggccactgtt
     6181 atagttaaaa acagaaccag gagatgaagt catcggacga ctcagaataa attcaatcca
     6241 gtcatcgctt tgattaagca aattgtgatc attcagcggg tcgttgtaag gtaatgctga
     6301 ttcattccat tgaagtccgg aggacatggt caataaattt tgaacggtaa ttgattcttt
     6361 ttcatctccc caatccaggt tggcaagctc cggaaagaaa ttaataaccg gtgtatccgc
     6421 taaaagatta tattgatcga cggcgatacc ggccagcgcg gaagtaatgc ttttggttac
     6481 agattgtatg gcggtgatac cctgggcgga ttcctgaaaa tactcttcta aaaccagata
     6541 attattttta gcgataagaa ctgaattcac ggagtaattt cccccgttta gctcatctat
     6601 cattgttgac agcaatttca cattgaatcc ctgctcttta gctgagccgg taatgatgcc
     6661 atcactcttc tgcaatggag tttccagtac tacttcgtta tcttctgagc atgcgttgat
     6721 ccccagtaat ac
//
LOCUS       DASWHX010000093         4800 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_444899, whole genome shotgun sequence.
ACCESSION   DASWHX010000093 DASWHX010000000
VERSION     DASWHX010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 4800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4800
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_444899"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2130)
                     /locus_tag="VGA21_06310"
     CDS             complement(<1..2130)
                     /locus_tag="VGA21_06310"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HEX9650153.1"
                     /translation="MIIIRCVLIISGVLLFTTCTQKLTVDEGVSLELAKSLKQQIQNI
                     IYKLDLTIPSGRKTPITGIMKIRFDYSGKGALILDFKADAENIKKVEMNGPVSWSTEK
                     GHIIIPTSELVEGENEVTINFRAGDMSLNRNNDFLYTLFVPDRASTAFPLFDQPDLKA
                     IFNLTLTIPNDWVALANGELLAVDSTAENSTWYFGETTPLSTYLVAFVAGKFTALTHE
                     RNGREMTMLHRETDTAKVNRNLKEIFNLHFQALEWLEDYTGIEYPFQKFGFALIPSFQ
                     YGGMEHPGGILYNASGLFLDESYTQNQLLGRASVIAHETAHMWFGDLVTMEWFNDVWM
                     KEVFANFMAAKIINPAFPDIDHNLRFLMAHYPSAYSVDRTPGANAIQQDLDNLKNAGQ
                     MYGAIIYQKAPIVMRHLESFVGEKIFRVSLQEYLKTYSYDNANWDDLVNIIDNNSDQD
                     VKVWSKAWVKEPGMPTLETIYSPENRSLTIVQNDDLTLRPQPVSYRLLYTGDEVIQRL
                     FLSGRQAELILENSAEPIVIIPNPDGFSYGYFKLDAQAISYLLIHAAEISDPVLRGSI
                     YIMMNEAMMNFEIQPESLLNAITKFLPVENELQNSQYILGVLGNIFWRYLPLYTRENV
                     CNSIEELLLDELEKRGSSREKAMIYNAFVNVATSSQSIGLMKSWWNDSKKIPGLTLSE
                     NDYTQLAYELAVRGADDSEIILKTQSGN"
     gene            complement(2140..2502)
                     /locus_tag="VGA21_06315"
     CDS             complement(2140..2502)
                     /locus_tag="VGA21_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014771623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX9650154.1"
                     /translation="MTINLNDKSKVIKEYWHPYIVGELNENYVKLARLKGEFVWHKHD
                     DEDELFFIVQGNLIMDFRDKTITAKQGEVLIVPKGVEHRPRTEGNMETVVMLVEPKST
                     LHTGDVVTEKTVTELEWI"
     gene            2631..3839
                     /locus_tag="VGA21_06320"
     CDS             2631..3839
                     /locus_tag="VGA21_06320"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEX9650155.1"
                     /translation="MNKSVNPVVCTLIVLTIYTISCEPKKKAPMTAGEFVEFLQNEAP
                     KIENKEGWNKFLETFSNPRMNNDSVILMYYGKAESVQWAGDFNGWSQHEPKALGIRVE
                     GSDIWFTTIHLPPDARVDYKIIVDGNWILDPDNELKQMAGVGGGSYNSELRMPAYIVD
                     TIGNLRTNIPKGSLSNAYSLQSHSMDYELNYQVYVPHNYENLDNLPSLYVTDGHEYSR
                     PELGNMTTILDNLIEDKRIQPLLVVFVDPRNPDKLNENRRMSEYVLNEAFVKFFTDEL
                     IPEIDSKFKTSTFAADRGILGTSLGGLNSAYFHATANNYFKRIGINSPAFFYKEEIFD
                     IYDTLKAIPSRIIITTGTMYDTQPAALRMKAIMERKDYPLLYIEVNQGHSWGNWEELL
                     DEVLVYLYGN"
     gene            complement(3974..>4800)
                     /locus_tag="VGA21_06325"
     CDS             complement(3974..>4800)
                     /locus_tag="VGA21_06325"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX9650156.1"
                     /translation="MITGLYLQNLDIKLLAGKYFSEEMLTAGGEKFVILNEKAVRLFG
                     FADNSAALDEVIYVDDSVMLEVVGVTESFHFRPFNYEIGPIAFRLRPDAAKILSIRFQ
                     PGTNPEQLVSAVLSVYRKFLPNHEMEWSLMDNEIKDAYEDSGLTDISKIVGYISFLAI
                     SISCLGLLGMVMFIVQTRSKEIGMRKILGASVSQLVIILNKSSIIILAIAFAIGLPVS
                     YLLGDWFLSIYAYRIPIDFSLILTGVGVIMAIVFITIGSQTLPTAKSSPLKWIRYE"
BASE COUNT         1396 a          998 c          976 g         1430 t
ORIGIN      
        1 atttcctgac tgggttttta aaattatttc tgaatcatcc gctccccgca ccgccagctc
       61 gtatgccagt tgtgtgtaat cattttctga gagggttaaa cccggaatct ttttggagtc
      121 gttccaccag gatttcatca acccgattga ttgggatgaa gtagccacat taacaaatgc
      181 gttgtaaatc atcgcttttt ctcgtgaaga tcctcttttt tccaattcat caaggagtaa
      241 ttcctcaatt gaattgcaaa cattttccct ggtataaagc gggagatagc gccagaatat
      301 gttgcctaaa actcccagga tatattggga attttgcaat tcattttcga caggcaaaaa
      361 ttttgtaatt gcattgagta aagattccgg ctggatttca aaattcatca ttgcctcatt
      421 catcatgata taaatggaac cgcgcagaac aggatctgaa atttcagctg catgaatcaa
      481 aaggtaacta atagcctggg catcaagctt aaagtagcca tagctaaaac catctggatt
      541 aggaataata actatgggtt cagcgctgtt ctccagtatc agttcagctt gtctcccact
      601 cagaaataat cgttgaatca cctcatcgcc ggtgtataac aatctataag aaactggctg
      661 tggtctaagg gtcagatcat cattctggac aatcgtgagg ctgcgattct ccggcgaata
      721 gattgtttca agagttggca ttccgggttc ttttacccag gccttgctcc atacttttac
      781 atcctggtct gaattattat cgatgatgtt aaccaggtca tcccagttgg cattgtcgta
      841 ggaatacgtt tttaggtatt cctgcaaact cactctgaaa attttttccc ccacaaaact
      901 ttcaagatga cgcatcacta tgggtgcttt ttgataaata atagctccgt acatttgtcc
      961 cgcattttta aggttatcca ggtcctgctg tatagcgtta gctccggggg ttcgatccac
     1021 ggaatacgcg gaaggataat gcgccatcag aaacctgaga ttatgatcaa tatccggaaa
     1081 ggccgggttg attattttcg cagccatgaa attagcaaac acctctttca tccagacatc
     1141 attgaaccat tccatagtta ccaggtcgcc gaaccacatg tgggcggttt catgcgcgat
     1201 tacgctggcc cggccaagta attggttttg ggtgtaagat tcatcaagga aaagcccgga
     1261 agcattgtaa agaattccgc cgggatgctc catgccacca tactgaaaag aaggaataag
     1321 agcgaaaccg aatttttgaa agggatattc tattccggta taatcttcca gccattccaa
     1381 cgcctgaaaa tgaaggttaa aaatctcttt cagatttctg ttaactttgg cggtatcggt
     1441 ttcacggtgc agcatagtca tttcccggcc attcctttca tgcgtaagcg cggtaaattt
     1501 acccgctacg aaagcgacca aataagtgct gaggggagta gtttcaccaa aataccaggt
     1561 agaattttcc gcggtgctgt caaccgccag taattctccg ttggcaagtg caacccagtc
     1621 attgggtatt gtcagggtga gattaaatat agctttcaaa tccggttgat caaataaggg
     1681 aaaggccgtg gaggcccggt cgggtacgaa aagcgtatac aagaaatcat tgttgcggtt
     1741 aagtgacata tctccggctc taaaattaat ggtgacctca ttttcgcctt ctacaagctc
     1801 tgaggttggt attattatgt gacctttttc tgttgaccag gaaaccgggc cgttcatttc
     1861 aaccttttta atattttctg catcagcctt aaaatccagg attaaagctc ctttgcctga
     1921 ataatcaaat ctgattttca taattccggt aatcggagtc tttcttccgg aaggaatagt
     1981 caagtcaagt ttgtaaatga tgttttgaat ttgttgtttg agggatttgg ccagttcgag
     2041 tgaaactcct tcatcgaccg ttaatttttg agtacaagtg gtgaagagta agacacctga
     2101 aataattagc acgcatctaa tgataatcat aagagaattt taaatccatt caagttctgt
     2161 taccgtcttt tccgtcacca catcacctgt atgcaaagta gatttaggct ccacaagcat
     2221 tactactgtt tccatgttac cctccgttcg gggacggtgt tcaactcctt tgggtacgat
     2281 caaaacttct ccctgtttgg ctgttatggt tttatctctg aaatccatga taagattccc
     2341 ttgcacgatg aagaaaagct cgtcttcgtc atcatgttta tgccagacaa attctccttt
     2401 taaccgggca agtttcacat aattttcatt gagttctcca acaatgtagg gatgccaata
     2461 ctctttaatc acctttgatt tatcgttaag atttattgtc atgaatttta tattatattg
     2521 gttatgaatt caatttaaag aacttcccta gtatttatac aaaggatgag gtattttact
     2581 ttccatgttc ttttattcag tctgattctt cttcctgaat gttttgcccc atgaataaat
     2641 ctgtaaatcc ggtggtttgt acgcttattg tcctgaccat atatacaatt tcctgtgaac
     2701 ctaagaaaaa ggccccaatg actgcaggtg aatttgtaga gtttcttcaa aatgaagccc
     2761 cgaaaattga aaacaaggag ggctggaata aatttcttga aactttctcg aacccgcgca
     2821 tgaacaatga ttcggtaatt ttaatgtatt acggcaaagc ggaatctgtt caatgggcgg
     2881 gtgattttaa tggctggtca cagcacgagc caaaagccct gggaatccgg gttgagggat
     2941 ctgatatctg gtttactacc atacatttac ccccggatgc acgtgttgat tataaaatca
     3001 ttgtggatgg taactggatt ctcgatccgg ataatgagtt aaaacaaatg gccggagttg
     3061 gcggtggatc gtacaattca gaattacgca tgccggctta catcgtagat accatcggaa
     3121 atttaagaac aaatataccc aaagggtccc tcagcaatgc ttattcactt caaagccatt
     3181 ccatggatta tgaacttaat taccaggtat atgttccgca taattatgaa aatctagaca
     3241 atcttccgtc actttatgtt acggatggac atgagtacag tcgtccggaa ctggggaata
     3301 tgaccactat ccttgacaac ctgatagaag ataagagaat tcagccgctg ctggttgttt
     3361 tcgttgatcc gcgcaacccg gataaactta atgagaaccg gagaatgtcg gaatatgtac
     3421 tgaatgaagc atttgtcaaa ttctttactg acgaattaat tccggagatt gattccaaat
     3481 ttaagacttc cacatttgcc gccgatcgcg gaattttggg tacttcgctg gggggattga
     3541 attccgcata ctttcatgca acagcgaata actattttaa acggataggg atcaattccc
     3601 ctgcgttttt ctataaagaa gaaatatttg atatctatga tacgctaaag gcaattccat
     3661 cccgcatcat tataacaacc ggaacaatgt acgacaccca gcctgctgca ctcagaatga
     3721 aagccatcat ggaacgcaaa gattatcctt tactttatat tgaagttaat cagggtcata
     3781 gttggggaaa ctgggaagaa ttactggacg aggtgcttgt ttatctttat gggaattgaa
     3841 aaaagattac agtactgatt aaaaaccaaa ttttcgccta aaataagaag tggaatagat
     3901 ccgccgactg ggtgagtgtt ccttattcta aacgtcagtg ttgaaaattt gtaagattat
     3961 gctcattcag gattcactca tagcgaatcc atttcaaagg gcttgacttt gccgtaggca
     4021 aggtctgtga accgatggta ataaatacta ttgccataat tacaccaaca ccggttaaaa
     4081 tcaagctgaa gtcaattgga attctgtagg cataaatgct aagaaaccaa tcacccaaaa
     4141 ggtaacttac cggtaacccg atggcaaagg caatggccag aattattatc gatgatttat
     4201 taagaatgat aaccagttgg cttacgctgg cacctagtat tttccgcatt ccaatttctt
     4261 tggaccgggt ttgcacgatg aacataacca tcccgagaag cccaagacag gaaatgctga
     4321 tagccaaaaa tgaaatatat cccacaatct tgcttatgtc ggttaatcct gaatcctcat
     4381 aggcgtcttt aatttcattg tccatcaggg accattccat ttcatgatta ggcaggaatt
     4441 tccggtaaac cgacaggact gctgaaacca gctgttcagg atttgttcca ggttgaaaac
     4501 ggatgctcag aatcttggct gcatcgggcc gaagccggaa tgcaataggt ccgatttcat
     4561 aattgaatgg tctgaagtga aagctttctg ttacaccaac cacttccagc ataacagagt
     4621 catcgacata aattacttca tccagcgctg ctgaattatc tgcaaagcca aataaccgta
     4681 ctgctttttc attcaggatt acaaacttct ccccgccggc ggttaacatt tcttcagaaa
     4741 aatacttacc cgcaagcaat ttgatatcca gattttgaag atacaaaccg gtgatcataa
//
LOCUS       DASWHX010000094         3253 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_2305034, whole genome shotgun sequence.
ACCESSION   DASWHX010000094 DASWHX010000000
VERSION     DASWHX010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 3253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3253
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2305034"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..463
                     /locus_tag="VGA21_06330"
     CDS             <1..463
                     /locus_tag="VGA21_06330"
                     /inference="COORDINATES: protein motif:HMM:NF026253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HEX9650157.1"
                     /translation="EAQFLTVGYFNDGIAWARDANKKLGFIDKEGKWIIDPQFDFAVD
                     FDPGSELARVKVDDLWSYVTKDGESVVISDTELWEDFKEGLAMGRKGKMYGFFDKQGN
                     WIISPQSEGVRGFQNGYAAAKLSKKWGIIDKSGEWVINPVYDGIKDVEVIR"
     gene            668..880
                     /locus_tag="VGA21_06335"
     CDS             668..880
                     /locus_tag="VGA21_06335"
                     /inference="COORDINATES: protein motif:HMM:NF027249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEX9650158.1"
                     /translation="MRKYLVIYEKGPDGYSAYIPDLPGCTSAGADKKEVEENIVEAIK
                     LHLEAMNEAGYDIPESISESEMLVLS"
     gene            819..1073
                     /locus_tag="VGA21_06340"
     CDS             819..1073
                     /locus_tag="VGA21_06340"
                     /inference="COORDINATES: protein motif:HMM:NF019540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HEX9650159.1"
                     /translation="MKRGTIYPNLFQSLRCWFCPEEVKYKAVIELIEQDGWFQVRQKG
                     SHRSYKHKTKSGIVTIAYHRFSDEVPRGTLNSILKQSGLK"
     gene            complement(1233..2057)
                     /locus_tag="VGA21_06345"
     CDS             complement(1233..2057)
                     /locus_tag="VGA21_06345"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX9650160.1"
                     /translation="MNGYSLFKYPSHNDYPSHWDQYALKYAKLLKPGTLYYLHKRVMY
                     DIIEKFAFPDIDTKGLEILDVNCGTGNDFPYLLSKGNVTGIDGSQGMLNKAYEKHREQ
                     ILSQNLTLYHGNLEEFNESSLENKSFDLIYSITGGFSYINDDELSRVLKVFKAMLKPG
                     GYIITGHLNTFCLVESLIWLLRGKMKRILLRLKKQVKTKYGSTMYFRNKKMLDSIITS
                     HFSHVEYFPLICISPPYQTGINTKPKTFEVLRRIENFMVKNKFGVSWADQIVVLCR"
     gene            complement(2270..2530)
                     /locus_tag="VGA21_06350"
     CDS             complement(2270..2530)
                     /locus_tag="VGA21_06350"
                     /inference="COORDINATES: protein motif:HMM:NF024835.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX9650161.1"
                     /translation="MGLSKGERKKLSKSDLSRIREIGNKLKKIRREKGYTSYENFAFD
                     AHIPRMAVYRMEKGAADFNFSTLLKILDALNVKPKDFFKDLG"
     gene            2608..2883
                     /locus_tag="VGA21_06355"
     CDS             2608..2883
                     /locus_tag="VGA21_06355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650162.1"
                     /translation="MSSTKNTSEVEHYHLLAEKCITIVKKFFDPSIPLSTLSFKNMLF
                     CGLHDAYMHRDDSSVNDEHVASMYYLWEILKEIDDLEMESICTRMDL"
     gene            complement(3103..>3253)
                     /gene="nadA"
                     /locus_tag="VGA21_06360"
     CDS             complement(3103..>3253)
                     /gene="nadA"
                     /locus_tag="VGA21_06360"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020604457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HEX9650163.1"
                     /translation="CACSECPYMKMNTLEKLRDCLLQEIPEIQVKEETRVKAAMSLDR
                     MLRFS"
BASE COUNT          993 a          610 c          643 g         1007 t
ORIGIN      
        1 tgaagctcaa tttttgacag ttggctattt caacgatgga attgcatggg cacgggatgc
       61 taacaaaaag ttaggtttta ttgataagga aggaaaatgg ataattgatc ctcagtttga
      121 ttttgctgtg gacttcgatc ctggcagtga actagcccgg gttaaagttg atgacttatg
      181 gtcgtatgta actaaggatg gcgagagtgt ggttatatct gatacagagc tttgggagga
      241 tttcaaagag ggcttggcaa tgggaagaaa ggggaagatg tatggcttct ttgataaaca
      301 aggaaattgg atcatttcac cccaatctga aggagttcgc ggatttcaga atggttatgc
      361 agccgcaaag ctgagtaaga aatggggaat tattgataag tcaggagaat gggtaatcaa
      421 tcctgtttat gatggtataa aggatgttga ggttattcgg tgagattatg catcccctta
      481 atgtgacgtg gtggtgaaaa caccgagggc gatgaggcat ccccagagtt ggagatgttg
      541 gtgctaacac caacaccggc gataggaaaa gcaactaatt gaattggtca ccagtgtttc
      601 tttacctgcg caatcaaacg tcctcgcttc aattattttt tttaccttta cataaaccat
      661 aagtaaaatg agaaaatatc ttgttattta cgaaaaagga ccggatggat acagtgctta
      721 cattcctgat cttccgggtt gcacgtctgc aggggctgat aaaaaggaag ttgaagaaaa
      781 tatagttgag gccataaaac ttcacctgga agcaatgaat gaagcggggt acgatatacc
      841 cgaatctatt tcagagtctg agatgctggt tttgtcctga agaggtgaaa tacaaagcag
      901 taattgaatt aatagaacaa gatggttggt ttcaggtacg acaaaaagga agccacagat
      961 cttacaagca taaaacaaaa tctggaattg ttacgatagc atatcataga ttttccgacg
     1021 aagttccaag aggaacgtta aatagtattc ttaaacaatc gggattaaaa tagccttgtt
     1081 gagaaaggtt cgtgtcccta cgaaccaagt tagtgaagct aatgggcgtt acaaacgatt
     1141 acgctggcac ctcattacaa acgagggcca tagttggggt caaaatttat tggaaatttc
     1201 ttgccgagta aatttactgg cattcaaaaa ccctatctgc acaacactac aatctgatct
     1261 gcccaggaaa caccaaattt atttttcacc atgaagttct caatccttct aagaacttca
     1321 aaggttttag gtttggtatt tattccggtc tgataggggg gagaaataca aatcaacggg
     1381 aaatattcta catggctgaa atgggaagtt attattgaat ctaacatttt cttattccta
     1441 aagtacattg ttgacccata tttggttttc acttgcttct taagtcttag caaaatcctt
     1501 ttcatttttc ctcttagcaa ccaaattaat gattcgacta aacaaaaggt atttaagtga
     1561 cctgtaataa tatagccccc tggtttcaac attgctttaa atactttgag gaccctggat
     1621 aattcatcat cattaatata actgaaacct cctgttattg aataaatgag atcaaagctt
     1681 ttgttctcca aagacgattc attgaattct tcaagattac catgatataa agttaggttt
     1741 tggctgagga tttgctctct atgtttttcg taagctttat ttagcatgcc ttgcgaacca
     1801 tcaattccgg ttacattacc ttttgataat aaataaggaa agtcattacc tgttccacaa
     1861 ttcacatcta atatctctaa tcctttggta tctatatcag ggaatgcaaa cttttcaata
     1921 atatcataca tcaccctctt atgcagataa tagagggttc caggctttaa tagtttagca
     1981 tatttaagag catattgatc ccaatgggag gggtaatcat tatgggaggg atatttaaaa
     2041 agcgaataac cgttcatcaa aaaggagtaa atctttcaat ctccaaaaaa taggtaaaat
     2101 attattctaa taatcaaaga atttatatcc attcctctcc acagccgcgg tctgtgtctc
     2161 acaaaccatc catccttctc catatgccct atttagatct catcaaataa atctttcaat
     2221 aaatcatctt agcctttgtt cgtgtctcac gaaccaagta agtgaaattt caacctaaat
     2281 ccttaaaaaa atctttgggt ttcacattca aggcatccag aatcttaaga agtgtactaa
     2341 aattgaaatc agctgctcct ttttccatcc tgtaaactgc catcctggga atatgagcat
     2401 caaatgcgaa attctcatag gaggtatagc ctttttccct tcttattttc tttagcttat
     2461 ttcctatttc ccgaatacgg cttaagtcag actttgataa tttctttctt tcgccttttg
     2521 ataatcccat tgtgcaattt ggtcatgatg taccaaatat gttactccat acgttgtaca
     2581 taaataacat tttactatct ttgcagcatg tcaagcacca aaaataccag cgaagtcgag
     2641 cactatcatt tacttgcaga aaaatgcatc accatcgtaa agaaattctt cgatccttca
     2701 atacctctca gtaccctgag ttttaagaat atgctgttct gcggcctcca tgatgcgtat
     2761 atgcaccggg atgattcctc ggttaatgat gaacatgtgg cttctatgta ttatctctgg
     2821 gagatcctga aagagattga tgatctggaa atggagagta tttgtactcg catggatctt
     2881 tagccaggtt gttatgattt ggttaaagaa aatggttttt tcgtcttcga cggggtccac
     2941 tacgggagac cccgcgagga cggaaaactg cttctgcacc atgactccag ccattggtgg
     3001 gctgaatagc ttccagttct gagcaaaggg tagagtaatg cgcgccaacg ttttaaggga
     3061 cttgtaatag tagcttaatt cctagatttc attaatatta ccttagctga agcgtagcat
     3121 ccggtccaat gacatggctg ccttcacacg tgtttcttct ttcacctgta tctcaggtat
     3181 ttcctgcaac aggcaatcac gcaatttctc cagtgtattc attttcatat aaggacactc
     3241 actgcaggca cat
//
LOCUS       DASWHX010000095        33676 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_3045063, whole genome shotgun sequence.
ACCESSION   DASWHX010000095 DASWHX010000000
VERSION     DASWHX010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 33676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33676
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3045063"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1551
                     /locus_tag="VGA21_06365"
     CDS             <1..1551
                     /locus_tag="VGA21_06365"
                     /inference="COORDINATES: protein motif:HMM:NF021126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HEX9650164.1"
                     /translation="LDSDSNLGQALRNNGVAPGQINQYLQNVPTYWGKQPATAPYYLG
                     AIMVFLFVFGLLQKNPYLKIWITSSIILGILLSWGSNFSGFNYFMFDHFPGYNKFRSV
                     SFALVIPIFLAPVLSSIGLNNFLKNPNQELLKKLLYALAITGGFALLVIIFAGAASFR
                     GAVDAQLAEQPSWFLQALREDRESLMRGDAIRSLILILLSGGLIWLIFMKKVSQNAGI
                     AGIILLALIDSFSVSKRYLNEDNFKERAIEQQFTQTEADQRILQDNTPGYRVYNLLGP
                     FNEARTSYFHHSIGGYHGAKIRRYQDLIENCLAEETNDLISDLRSGIRQFEDYPVINM
                     LNAKYLVAGARESSVLVNSSAYGAAWLATEVIKVQSADEELDAVCSIKSKQQAVIDIS
                     RFNVGESSFSGLGTVSLDSYAPNYLTYSAKVEGASLLIFSEIYYPEGWSATINGEPLQ
                     ILRANYVLRAAVVPAGDHVIKFTFAPGSYSKGNKVMLIFSILIICFFFYGLYSEYKES
                     KKVELKKL"
     gene            1591..2109
                     /locus_tag="VGA21_06370"
     CDS             1591..2109
                     /locus_tag="VGA21_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009581959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEX9650165.1"
                     /translation="MNSYKERRYTKTISFLEKVAPPPLRILDLGTENPFSEIMKQKGY
                     EVCNTNGEDLDVEFDVVNHYRVDMVAAFEIFEHMLAPFNILKEIKCSRMVASVPLKLW
                     FASAYWNEKDDWDKHYHEFEPKQFDFLLRKTGWKIVASEKWTSANPWKFGIRPFLRNF
                     VPRYYIVYCEKI"
     gene            2109..3596
                     /locus_tag="VGA21_06375"
     CDS             2109..3596
                     /locus_tag="VGA21_06375"
                     /inference="COORDINATES: protein motif:HMM:NF014051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HEX9650166.1"
                     /translation="MGIVIRQSFYNSIFSYLGVIIGFINVIFLFPAFLSEVDIGLYRA
                     IQNASLLLLPFATFGLQASTLKFFPEAQQKESTQDQFLGFMLVAVTFFYLIFLAFYFL
                     FQSVIHQYFAEKAQPLIDSFNLVLILVFILSFQTVIEAYSRNLLKTIFPGFLRDFFFR
                     FLISVSVLLFAINLISFEALLNSLIGIYLIVLLLLLLYLYRDTNFQIRLPTTKAFGSL
                     GKRILGYSFFIFLGFSGSTIIVNVDSIMISSMLGLAQTGIYVTAMYMGNIIELPKRSI
                     GQITAPLLAKAFEEKNLKEVDSLYKKVSINQSVIGTYLLLGILVNLDNIFSIMPNSEV
                     FAAGRYVVLFIGLGKLIDMIFSFNGEIILFSHFYKFNVTATIILGVLTIATNLLLIPV
                     YGISGAAFASLLTLFLFNIIKFVFLKIKLNMQPFNFNTLKVIAFGLITYYLVSIIPQL
                     PVWIDLLIRSILVTVIFGSLILFFKCSEEISNLSQGGWKQLFKKN"
     gene            complement(3593..4678)
                     /gene="wecB"
                     /locus_tag="VGA21_06380"
     CDS             complement(3593..4678)
                     /gene="wecB"
                     /locus_tag="VGA21_06380"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HEX9650167.1"
                     /translation="MKKIITVVGARPQFIKAAVVSRHLRSLEGMSEIIIHTGQHFDPN
                     MSEIFFEEMEIPKPDQNLGINSRNEAAMTGEMILGLQEIFNDIRPDMVMVYGDTTSTL
                     AAAIAARKSGNRLSHVEAGLRSFNRSMPEETNRILTDHVSDLLFCPTKHAVSNLLREG
                     FNHEEQKIVLSGDVMLDAFMFYRQKSLERVSSSIDGIVEKGNYVLLTLHRQSNVDDTK
                     TLSQLLHAIGKLSRSIRVVFPCHPRTRKKMELIEVENGIELIDPVGYLDMIKLLENCE
                     MVLTDSGGLQKESYFAGKFCLTLRNETEWVELVEGGFNFLVGGKLDQLETFYENLGSR
                     EWNHQSHLYGNGNSAKIIADEVVTFLH"
     gene            complement(4675..5961)
                     /locus_tag="VGA21_06385"
     CDS             complement(4675..5961)
                     /locus_tag="VGA21_06385"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX9650168.1"
                     /translation="MKKVLIITYYWPPSGGSGVQRWLKFAKYLRGFSWESIIYTPHQG
                     EFPVIDKSLEKDVPQNLEIIKTPIREPYNLYKKITGNKDAQTVSFLKEGKSGIIQKMF
                     QWVRGNFFIPDPRIFWVKPSIKFLAGYLKENPVNIVVTTGPPHSLHLIGLGLKRKLNV
                     TWVADFRDPWTNIDYYKDLYLTKWADKRHRELEKDVLSTADTVVTVGETLANELQNLG
                     AKRTVVITNGYDSDDYKSVSSVSNVFSFVHVGLLSKTRSHPALWRAFANFLQKIPEAK
                     SELKIKLVGKTDYSAIQEIKRAGIEAFVEKINYVDHEEVVRIQQDAWIFLLPINRTPN
                     SKGIISGKIFEYMAAGKPILAIGDTDGDVAQIISDSNSGVIFDFDDVSGISGYMEKMY
                     LEFKRNFRQVSTSNIEKYSRRELTQKLARELNHLSG"
     gene            complement(5961..6890)
                     /gene="miaA"
                     /locus_tag="VGA21_06390"
     CDS             complement(5961..6890)
                     /gene="miaA"
                     /locus_tag="VGA21_06390"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020529160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEX9650169.1"
                     /translation="MVTKRPMESENHCIIVICGPTGVGKTDLAIKLAQYFDTEIISAD
                     SRQIYMELNIGTAKPTNEQLSKVKHHLINYISVQEKFTAGDFERVALKIAAGIMNRKK
                     IAILCGGSGLYIKAFLEGFDDLPEVPSEIKKELQKEFETNGLSDMLNELEKKDPFYFE
                     EVDRRNPHRVIRALAVIRSSGLPFSELRSGKKKVRPFKVIKIGIGLSREVLYDTLNQR
                     LDSMIDDGLMDEARAMMKYKNESSLQTVGYNEAFEYLEGKITLEESVKLMKKNSRNYA
                     KRQFTWFRKDSGITWFEPNEYDLILEHIREHLN"
     gene            7105..7488
                     /locus_tag="VGA21_06395"
     CDS             7105..7488
                     /locus_tag="VGA21_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017732977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9650170.1"
                     /translation="MEKRILVVDDSSVMQNLVKKIFAQLDYEVVSAKNGNEALEKLKK
                     QDFNVILMDINMPVMDGMECTKQIRKLKNKKGQLPIIAITGNSKNYAWDDFKKAGMSK
                     YLQKPLNFDELVEIVNSYVKDDNKV"
     gene            7472..7747
                     /locus_tag="VGA21_06400"
     CDS             7472..7747
                     /locus_tag="VGA21_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009579049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650171.1"
                     /translation="MIIKYSQSFLNKLEDLFSETDYSLRYEKGNFKPGHCLIRDSRVA
                     VINKYYPLEGKINALVELIRSIEIRTDRLSEKNQKLFEELSQMEARI"
     gene            7744..8505
                     /locus_tag="VGA21_06405"
     CDS             7744..8505
                     /locus_tag="VGA21_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017732975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX9650172.1"
                     /translation="MKVTFLGTGTSQGVPVIACECEVCRSLDFRDNRTRSSVLIEYKG
                     VSILIDAGPDFRFQMLRERVKRIDAVLFTHAHKDHTAGLDDIRSFNFRQKSVIPIYGT
                     QEVLNQIKQEFRYIFDDKSYPGIPQLSLNVIDDQSFNIKGLEVIPIPVMHHKMPVLGF
                     RVGNFTYITDANDIPPLSRRDIEGTYTLVLNALQKEPHISHFTLEEAIQVSKELKIPQ
                     TYLTHISHRLGLHQKVSEEIPKGVALSYDGLQLTF"
     gene            8506..10050
                     /locus_tag="VGA21_06410"
     CDS             8506..10050
                     /locus_tag="VGA21_06410"
                     /inference="COORDINATES: protein motif:HMM:NF017633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT RNA binding domain-containing protein"
                     /protein_id="HEX9650173.1"
                     /translation="MHFNYFFLSKLAKSLNQRLAGAKILECFSQNKNELIITTLPVNE
                     MKFFHIRIDLNPNYSFVNFPENFYRSKRNSISLFEKITAAVIAGIRVINNDRSLIIEF
                     TNTYSLLLKFHGNQSNVILLEDFRVVSLFRKKLLKDKTLEFESLEKQINYSKNYFLTA
                     KANLDEYPTLGPFVKKRIKMLWQSAKSIDEQWKIFHKILLELEGENIYILREEGKLML
                     SLIKMGTVISTHSDPLTALNEFAAIFTRENNFNLKKKHAENLLRYEEKIHNQSLQSLT
                     TSLQKLAQQKNYKVYADLLMANLHKINPYDSEVTLSNFTDNSEITIPLKMGLTPQKNA
                     ENYYRKSKNQNIQITKNKELVKLKEKSVKEIRSSLELLNDAGTVKEIEEIIAPFEKLK
                     ADKSKSHPYKTFHFMDFEIRVGKNAKSNDLTTFKHSYKEDLWLHVKDAGGSHVIVKYK
                     SGMNFPRSVIERAAQLAAFNSKRKTETLCPVIYTPKKFVRKRKGDPAGVVVVEKEKVL
                     LVEPKF"
     gene            complement(10051..11478)
                     /gene="pyk"
                     /locus_tag="VGA21_06415"
     CDS             complement(10051..11478)
                     /gene="pyk"
                     /locus_tag="VGA21_06415"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010855326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEX9650174.1"
                     /translation="MSEILFNKTKIVATVGPATNSKSKLTELIKEGVDVIRLNFSHGS
                     HEDHFKVIQFVNEINEELKTNICLLQDLQGPKIRVGEMEPGVILEEGSEIKIRSGDGI
                     GNSEVIYTQFSELIDKAQPGHKLLLNDGQIELKVTEAGGKSLTALVLNGGELKSKKGI
                     NLPDTRVLVPSLTPKDLKDLQFGIEHDVDWVALSFVRSPKDIHDLREILNKSGKWIKI
                     IAKIEKPEALEFIDEIINTADAIMVARGDLGVETPMEEVPLIQKMIVEKCNIASKPVI
                     IATQMMESMIENPKPTRAETNDIANAVMDGADALMLSAETATGKYPLKVIQSMVKTII
                     SVESHDRLYFRFYPTDKNSPIFLNDSVVASSVVLAKETNAKAMVGMTQSGYTVYKVAS
                     YRPKTSIFAFTANKPLLRTLSLVWGVRGYYYDKSESTDSTISDIEEILIREGHLSKGD
                     VFINMASMPIHARERTNMLKINIVK"
     gene            complement(11471..11905)
                     /locus_tag="VGA21_06420"
     CDS             complement(11471..11905)
                     /locus_tag="VGA21_06420"
                     /inference="COORDINATES: protein motif:HMM:NF033205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPExxxVDY family protein"
                     /protein_id="HEX9650175.1"
                     /translation="MRKSKLVVDPLFDFDLVGLISHAKGYRIAWELNRTLEVQLIKAK
                     DQIIRFNDGNAITVVNYYYKTENAGLFLLRNRSIETKESGQMLLIPELQEFDFFLKIK
                     GADSFDAEEIAKIVRNIKIIQYSSLIDPINLKSKENLVFDYE"
     gene            12063..12302
                     /locus_tag="VGA21_06425"
     CDS             12063..12302
                     /locus_tag="VGA21_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009195174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEX9650176.1"
                     /translation="MSEIAQKVKSIIIDKLGVEESEVTPEASFTNDLGADSLDTVELI
                     MEFEKEFNIAIPDDQAENISTVGQAISYLEENVKQ"
     gene            12331..13584
                     /gene="fabF"
                     /locus_tag="VGA21_06430"
     CDS             12331..13584
                     /gene="fabF"
                     /locus_tag="VGA21_06430"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018473100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEX9650177.1"
                     /translation="MDFKRVVVTGVGALTPIGNTANDFWQGLSNGVSGAARITKFNPE
                     KFRTKFACEVKGFDPNNFFERKDARKMDLFTQYALAVCSEVVKDSGLDLTSMNLDRAG
                     VIWGSGIGGLETFQEEVKSYAMGDGTPRFNPFFIPKMIADISAGQISIKYGFRGPNFV
                     TVSACASASNAIIDAFNYLRIGKADLFITGGSEAAVTEAGMGGFNAMKALSERNDSPE
                     TASRPFDKDRDGFVLGEGAGGIILEEYEHAKARGAKIYAELIGGGMSADAHHITAPHP
                     EGLGAFNVMINALEDAQITADQVDYINVHGTSTPLGDISEIMAIEKVFGESAYKMNIS
                     STKSMTGHLLGAAGAIEAVACILAIKYQTVPPTINHFTDDEQFDPKLNLTFNKVQQRT
                     INVALSNTFGFGGHNTSIIFKRMNE"
     gene            13577..14314
                     /gene="rnc"
                     /locus_tag="VGA21_06435"
     CDS             13577..14314
                     /gene="rnc"
                     /locus_tag="VGA21_06435"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009579056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEX9650178.1"
                     /translation="MSNFPIFGTTKKSSDYDRTLAKAIRAMIGKKPRNLDLFKLATLH
                     VSAAKENDNGVRESNERLEFLGDSVIDLVVTEFLFKKFPLKNEGFLTDIRARIVNRET
                     LNNLARKLGIDNLIQMDNSIKGVMAHKSLLGNTLEAILGAIYLDRGYPFTRQFILKKI
                     ISTHFDLEEIIKNNPNQKSKIIEWAQKENKTLTFNLVETIERNNRKQFIVTLEIEGRI
                     LGTGEGFTKKKAEQDAAQKSCEILNLD"
     gene            14318..16195
                     /gene="nadE"
                     /locus_tag="VGA21_06440"
     CDS             14318..16195
                     /gene="nadE"
                     /locus_tag="VGA21_06440"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014018611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HEX9650179.1"
                     /translation="MRKLRVAGGTVNQIPLDWDNNLFNISACIKEARTNKVDLLCLPE
                     LCLTGYGCEDLFLSDWVSKKSLRKLVSLLPQTKGIGVVIGLPIRIDKITYNTSCFIYD
                     GQIQGFYAKQNLANDGIYYEHRWFKPWTPGIIHTIKIENQEYPVGEIIFELKGIKLGL
                     EICEDIWVTERPACRYLEKGVDLILNSSASHFSMGKSLERQELVINSSNLFNCAYVYA
                     NLLGNESGQIIFDGDIILAQKGNLLAKGKTFGFESYRILSFDFDFDGGSTKNLDKVIQ
                     NCNVQFLQGESLALFDYLRKSRSKGFVISMSGGADSALCAVLVYQTIKSAVQELGLAG
                     FLDRIDRQDLQSVKNLPAKNQIPALAEELLILAYQATNNSSTETLNAAKELSANLNCK
                     LLHWNIDKSIEEYTSIVEEKIGKRLNWEEHDVTLQNIQARARSPIIWMIANYKNYLLL
                     VTSNRSEGSVGYATMDGDTSGSLAPIAGVSKKFIREWLEWAEKEFELPSLKTINNLKP
                     TAELRPPDRRQTDEDDLMPYTLLQEIESMAVKRKLSPVEVFQALKGTEKLSEKELADY
                     VIKFFRLLGHSQWKRERFAPSFHIDDYNINPRSWFRFPVLSSSFKDEIKELQDLINKS
                     N"
     gene            16229..17356
                     /locus_tag="VGA21_06445"
     CDS             16229..17356
                     /locus_tag="VGA21_06445"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017732966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEX9650180.1"
                     /translation="MFQPSYFLWNADPEIFEIFGRPIVWYGLLFACAFLLSQQVMYHI
                     YKKEGKRIEDVDTLTVYMIIAVILGARLGHVFFYEPMRYLKNPIDIIKVWEGGLASHG
                     AAVGILTALWIYTRYSIKWSGTRLKVKKQKRENQSFLQVVDRIVIVVALSGCLIRFGN
                     FMNSEIVGLPTHSNFGVMFMRNVTDAIQSNNPGVESVEYSKRAGEPTEEGYEPIRIEL
                     VFNPDYSHNEEQVVLYLDNTLNNILGNSTYVTNNIHEPLIHNLDYTYRSRPDGKIVAT
                     IDTFGISRHPAQLYESISNLLIFIMLYLIWRKWQINAPPGKIFGIFLVVLFGLRFIYE
                     FMKENQVDFEQGLALNMGQWLSIPLVLTGAFLLLRPKSPSR"
     gene            17439..18251
                     /locus_tag="VGA21_06450"
     CDS             17439..18251
                     /locus_tag="VGA21_06450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650181.1"
                     /translation="MQYFTLFIFLFITAQLYGQSYFNLDPRSEGRANTFSVETGAGSL
                     FVNPSGSAMLEGFTAIFAGQNIAGLEGLHTSGIAIGQPTRFGVIGAIFGNTGDELLNH
                     SGLALNWAQKLGIAQLGLRTGIINTSIEGQKSKNSFQLDFSGIVKLSPILSVGAGISN
                     LTKSKYTGQEQIPVTGNFGLDIHPTRSFHLTMETEKDLAKKPEYKFGCSYSIKERIEI
                     LTGINIRPLRQSLGLGFTLRKFIVQWSLTNLPGLGSKQSISLCYKTSKKKDA"
     gene            18244..20250
                     /locus_tag="VGA21_06455"
     CDS             18244..20250
                     /locus_tag="VGA21_06455"
                     /inference="COORDINATES: protein motif:HMM:NF024245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEX9650182.1"
                     /translation="MRNWFTLLSIIVCSNAFGQSDEIDIERIINTNSILDDEVTSEEI
                     NALLLDILSSPLDLNTVSEEVLSGISFLSNEQIRNFIDYRNQFGPFISKWELQQIEGW
                     DVDVCKMVASVSIVDNKNNGKFLNNFGRNAEFIIRESRLLQERIGFADSINRFEGDRN
                     KLQLRFKSSNPGDYSVSLAVENDPGEKFRFRSGRLGFDHISGNVSLFNRGFIRKIVLG
                     DFRLQFGQGLIFGSSFGGRKSSEPIMSLRKPNTGIIPSSSFSETGFLRGVAVSLGKKG
                     LNLTVFGSRISKDATLRFDSLKSVEFITSFNETGLHRNGKEVKINNTVNEILAGGAIA
                     FRKSKLETGLQYHAGKYSSPVMLDSKPYQLYRFQGRRFHAGGIFLNYGWANYYLFHES
                     AVSNFNGFSTLTGIILALSKNIDLSVLFRHFSPGYQSPYGKGFAETSTLDNETGLYWG
                     LKYHLLKFNISAYFDYFKFPWLKFGVDNPSDGYEYMIRIDYLSKQTPFYLLLKNEKKY
                     AGKELDSEPANIIYSERTNLTANFQFILTEHVSGNIRPAATYSNTKGGKASGVLLKQD
                     LKIEYNPFTLNFGYFLFDTSSFEGRLYSNEPDVRFQSSFPLFNGEGMRYFFLLKIKVR
                     KALNFWIKYYLTSYNNIDEIGSGPDLIKGNKISELKTQIIYSFR"
     gene            20257..21297
                     /locus_tag="VGA21_06460"
     CDS             20257..21297
                     /locus_tag="VGA21_06460"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX9650183.1"
                     /translation="MFEIFSYLGLIVGCGALFITALKTSILLRIVDFQSLSNWKVLRG
                     LIITFMLGYLFSLGAIASRPITFVEVITVTIFLAGSFFVLLVVNSAFRDIQLIRSSRN
                     LLQDRNFELQKLNIQLDTFLYSTAHDLRAPLTTILGLVDLGKRESSVTEKDYYFSMIK
                     NRVDTMDFLLKEIIGFTKNKHLEIHQEPVNFQKMIASVLAELEPVKMKDVTLELNIDF
                     DNEIWIDHQRFRSIIYNLLSNSFQFFDPKRSNAWVKVNASINGKELRVSVVDNGIGID
                     KRYQEKVFEMFYRASHNSAGSGLGLYITREIVVTLGGTIHLNSTQGVGTDVAICFPYP
                     LSPFKNSLTQTP"
     gene            complement(21269..22516)
                     /locus_tag="VGA21_06465"
     CDS             complement(21269..22516)
                     /locus_tag="VGA21_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018618874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX9650184.1"
                     /translation="MDKLLMVFGIALILLLIDYYLFDSTKKIRRSWPEAIRKVTIILF
                     WAISFIAIASLFGFNYAPNEGVMNGMRRFFLVFLFLNIFPKILALPFLMVDDIVRIIK
                     WTNKVIHKSEEKHLEGKKIKRSEFLVKAAIIAGGVPLAIMSYGIVSGAYNYRIIRRKL
                     VIPNLPKEFHGIKLAQISDIHAGSFFNKRAVAGGIEMLMGEKPDLIFFTGDLVNTQTD
                     EVNKYFDIFNKVKAPLGTYSILGNHDYGDYRSWASADAKRKNLLDMIEAHKLLGWDLL
                     LNENRTLELGGEKISIIGVENWGAGRFTKYGDIVKATQNINESSVKLLLSHDPSHWDA
                     QIRPDYSDIDVMFAGHTHGFQFGVELGNFKWSPAQYAYKQWADLYQKGNQKLYVNRGF
                     GFIGYPGRIGIAPEITVFELVNS"
     gene            complement(22525..25935)
                     /locus_tag="VGA21_06470"
     CDS             complement(22525..25935)
                     /locus_tag="VGA21_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017732955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX9650185.1"
                     /translation="MESKYSNQKKIDKEFGLTTFAVKNRTSVVVLTFIIVVLGVGAYI
                     SMPKEAFPEVKIPTIYVGTIHPGNSPEDMESLITRPLEKEINTIAEVDNIKSTSVQDY
                     STVIVEFTTDTDVEDALLKVKDAVDKAKQELPDDLQADPDVFELDFSEFPILNINLSG
                     DFSLEELNKFGEYLEDEIEGFPEISKVDIRGVDEKEVKIIADPYKLEARKLSFQDIES
                     AVAAENLNLSGGNILANGVRRNIRVLGEYKDPSELLNIVISNENDRIVYLKDVARVEF
                     DYKDKESYARLNGNPVVMLDVVKRSGENLLLVTDNIFAVLAKAQAEVFPPGVKISITN
                     DLSDQTREQVNSLENSIIFGVILVVLVLLFFLGTRNALFVGIAIPLSMFMAFMILQAV
                     GVTINMMVLFSLIMALGMLVDNGIVVVENVYRMFEEGYSPKDATIKGVGEVAWPVIAS
                     TATTLAAFLPLAFWPGIMGEFMKYLPITLIITLGSSLFVALIINPVLISVLMRLDDPT
                     KINYKKGLRTSAIALVLGILFLLVNITWLGNLLIFFALLLPLNLFVLTPLSRRFQKTF
                     LPRLENLYVKIITYALAGRRPFAFLGGTFFLFIFSFFLMGVFQPNVIFFPVNQPLYVN
                     VFIEFPVGTDITTTNEFTSMIEKKVTKIVEPYDYMVESIISNVGNGASDPNDMGSIGA
                     GQTPNKGRITVNFLEYKLRKGVQTSDIMSEIRKGISNYPGVTITVDKDAAGPPTGKPV
                     NIEVSGRDYPTLIAISENLKAFINESKIAGIEKLKTNLESGKPELIVEIDREKARRFG
                     LSTGSIASEIRTALFGKEISKFKQGEDDYEVQLRLEDKYRYDVDVLMNKSVTYRNQNN
                     GKIYQVPISSVASTEFTSSLGSIHRIDLDRVITISSNVLDGYNPTDINNHIKDLIQDF
                     EVPAGYTIAFTGEQEKQAEEFAFLSKALMLAVFMIFLIIVSQFNKVSIPFIIMSSVLF
                     STIGVLLGLIIFQMDFIVIMTMIGIISLAGIVVNNAIVLMDFIELTRKRKREELGTDQ
                     RLPPEEIRNAIIQAGRTRLRPVLLTAITTILGLIPLAVGLNIDFIHFAQNYDPDFYLG
                     GDNVIFWGPMSWTIIFGLTFATFLTLVIVPVMYLLTDKLNTKLGISKY"
     gene            complement(25937..27094)
                     /locus_tag="VGA21_06475"
     CDS             complement(25937..27094)
                     /locus_tag="VGA21_06475"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX9650186.1"
                     /translation="MKAISSLSIIFLLFVSASCGQTEKKSELETMKAQLDIYNRQLVD
                     LESKVKALERQIVQADPDFAGTLSNYTLVSTIPVVKKKFIHQIQARAIVESRKNIVVS
                     SESMGRITSIKVKEGDAVSEGQVLLTVDAEILKNNIEELKTSMELAKVIYERQENLWQ
                     NNIGTEIQYLQAKNNYESLERKLETAKSQLAQSVIRAPFSGHIDEVFIKEGEMAQMGG
                     PVVRIVSLKDMYLKADISESHIGKFKANDPVQVEFPSLNRKFESKITAVGQMIDPSNR
                     TFSLEIKLLNMDALIKPNLTALVSLTDYENPEALVVPTEIIQKDNKGDYVYVVQNGND
                     GTMATRVHIEIGKSFQNESEILAGLSNTAFVINKGFREVTDGMMVKEVKAN"
     gene            complement(27102..28445)
                     /locus_tag="VGA21_06480"
     CDS             complement(27102..28445)
                     /locus_tag="VGA21_06480"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX9650187.1"
                     /translation="MRLTLLMVGFLLPLKAIPQEKIINRFTLEECIEYALKHNEEYLN
                     AGLEASAQETFVGEVLSDGLPQISGEINLINNFRVQTSFIPAIFFDPNAGDDDFVPVQ
                     FQTQYNGGASVTFSQLILDGAYFLGLKAARTFTQLSAKDFIKSKIDLKADVSKAYYSV
                     LVFKEGLELIKNNYQRIDTLLKETQLLFENGFAEKIDINRLKVEHNNLKTEMENMERS
                     LIASYLVLKFQVSMPDNEELVLTEDLSQITFDLNAEDLSEFTYEDRIEYSTLNTGLEL
                     AHMDLKNIRLGYLPKLDGFATFGANAGTGSWGDLFAVGDNWFTYGLYGFSLSVPVFDG
                     FRKSNQMQKRKIIINQRENQKQRLEKAIDMEIRTARISLENSVARMSTQKENMGLAEE
                     VFEISRAKYQQGLGSNLEVIEAENSYKTSQTNYYNALYEALVAKIDYQKALGRLN"
     gene            complement(28442..29050)
                     /locus_tag="VGA21_06485"
     CDS             complement(28442..29050)
                     /locus_tag="VGA21_06485"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX9650188.1"
                     /translation="MKEKIIEGAGNLFVRYGIRSVSMDDIANNLAISKKTLYQYFKDK
                     DDVVSQATMAIIEKDRGVFLEMKESSANAIEELSKVSGCLRRMFDEMNPSLLFDLNKY
                     HPDAWEYYLKYKNEFIFQMITDNLTRGVKEGNFRDDININILARLRVEQVQICFDQKV
                     FPKDQFDFKEVQMQVFDHFLFGIVTSKGKALYEKYQIKNSVL"
     gene            29291..32977
                     /gene="purL"
                     /locus_tag="VGA21_06490"
                     /gene_synonym="purI"
     CDS             29291..32977
                     /gene="purL"
                     /locus_tag="VGA21_06490"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009579082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HEX9650189.1"
                     /translation="MIIFFTTTRKVTYAVQSGSQLSKNDLTKLEWLFSARYIADNQLD
                     TDYIGPRKEMVTPWSTNAVEITQNMGVPGITRIEEFHLADNKTEYDRMLQHFYGELNQ
                     EIFRINRAAEATKEIEDIGAYNVSQGLALNDDEVTYLELVGKEIGRNLTDSEVFGFSQ
                     VNSEHCRHKIFNGRFIIDGETMPESLFELIKKTSAQNPNRIVSAYKDNVAFVKGPEVE
                     QFSPGRQDIPDTFKINTFDSTLSLKAETHNFPTTVEPFNGAATGSGGEIRDRMAGGKG
                     SVPLAGTAVYMTSYPRLAESHLWEKKTEARKWLYQRPLDILIKASNGASDFGNKFGQP
                     LICGSILTFEFINDSVSFGYDKVIMLAGGIGFGKLKDTQKDIPAKGDKVIVMGGDNYR
                     IGMGGSAVSSVATGEYGDAIELNAIQRSNPEMQKRVFNAIRSLTEMAVNPVISIHDHG
                     AGGHLNCLTELLESTGGVIDISKLPLGDPTLSDKEIIGNESQERMGLLIKEKDLATFS
                     KIAEREKAPYYIVGEATGDHIFKFINGRNGSNPIDLKVNHFFGSSPKTILEDKDAQIE
                     YDKLFYDIQAVETYLQDVLRLESVACKDWLTNKVDRCVTGKVSQQQTIGPVQLPLSDV
                     SVMALDYRGEKGVATSIGHAPVSGLIDPAAGSVLSAAEALTNLVWAPIEGGLKGVSLS
                     ANWMWPAKNPGENARLYKAVKALSDFVCDLGINIPTGKDSLSMSQKYPDGKKVLSPGT
                     VIISTIGEVIDIKKVITPLLQKVESEIIYIDFSGVEFKLGGSSFMQCLGQLGGETPTV
                     NSAEYFARCFDAIQELMSEDLILAGHDISSGGIITCLLEMCFTRNDIGIKANFEFEGL
                     DAIQILFSENPGVLIQVENAKKAEKKLKKAGVKSLKVGEIIFESHLQLKVNEFDYKLD
                     VRKLRNAWFETSHLFDLHQTRKEFALRRLANYGQQELSYILPSDWQGTYESLDIKPFR
                     KERTGIKAAIIREKGVNGDREMAWALHMAGFDVLDVHMTDLMEGRENLEDISFLVFVG
                     GFSNSDVLGSAKGWAGSFMFNANAKQALDNFYEREDTLSLGVCNGCQLMMELRLLYPE
                     MNPHPVMKHNAGGKFECHFLSIDVPENNSIMFKSLGGTRLGIWMAHGEGRFELPGKEE
                     DYNIAARYTFGEFPGNPNGSDFAVAALSSKDGRHLAIMPHLERSLFPWNWAYYHESRK
                     TDQVSPWLEAFINVRNWVKSKVSI"
     gene            33106..33669
                     /locus_tag="VGA21_06495"
     CDS             33106..33669
                     /locus_tag="VGA21_06495"
                     /inference="COORDINATES: protein motif:HMM:NF024978.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4136 domain-containing protein"
                     /protein_id="HEX9650190.1"
                     /translation="MRKFFLPLALLISACSVYEELPSDYDYSFKGKFRTYDTFAFFQE
                     QSAQDDGTLFTKESIENVIAARMRSQGYSQRDKNPNLLVMYKIFYDSLRLTGYKQPEL
                     EEWIKYEDENIEYVERKYQLRMGTLYISFFDRKQKRTVWQGYATKVYGNDMFTNERLL
                     RNAVRSVLNRYKVVSESFNKTIKERRF"
BASE COUNT        10168 a         6606 c         6522 g        10380 t
ORIGIN      
        1 ctggacagcg atagtaattt ggggcaggct ttgcgaaaca acggcgttgc tccgggtcag
       61 ataaatcagt atttgcaaaa tgttcctact tactggggca aacagcctgc tactgcaccc
      121 tattatttgg gagcgataat ggtattccta ttcgtgttcg gattattaca gaaaaatcca
      181 tacctcaaaa tttggattac gagcagcatt attctcggta tactgctaag ctggggaagt
      241 aacttctccg gttttaatta ttttatgttt gaccactttc cagggtataa taaatttcgc
      301 tcggtatcct ttgctttagt aatacccatt tttcttgctc ctgttctaag cagtatcggc
      361 ttaaataatt ttttgaagaa tccgaatcaa gaattgctta agaagttact ttatgccctg
      421 gcaatcaccg gtggttttgc cttgctggtt attatttttg ccggggcagc cagtttcagg
      481 ggagcagtgg atgcacagtt ggctgaacaa ccgtcatggt ttcttcaagc tttgagagaa
      541 gaccgtgaat ctttgatgag aggggatgcc atacgatcac ttatcttaat tttattgagc
      601 ggcggactta tatggctaat ctttatgaag aaggtctctc aaaatgccgg tattgccggg
      661 attattctgc tcgctctaat tgattcattc agtgtaagta aacgatacct taacgaagac
      721 aatttcaaag aaagagccat tgaacaacag ttcacacaaa cagaagccga tcaaagaatt
      781 ttgcaggaca atactccagg gtacagggtt tataatttgc tggggccttt taacgaagcc
      841 cgaacctctt actttcacca ttccataggt ggatatcacg gtgctaaaat caggagatat
      901 caggatttga ttgaaaattg tttggctgaa gaaaccaatg atctaatcag cgatttacga
      961 tccggcattc gtcaatttga agattacccg gtcattaata tgctgaacgc gaaatatctc
     1021 gttgcaggag ccagagaatc ctcagttttg gtgaactcca gtgcctatgg agccgcgtgg
     1081 ctggcaacgg aggtaattaa agtacaaagt gctgatgaag agcttgatgc agtttgttca
     1141 attaagagta agcaacaggc ggtgatagat atttcaagat ttaatgttgg agaatcatca
     1201 ttctcaggtc ttggtacggt atctcttgat tcttatgccc ccaactattt aacctattca
     1261 gcaaaagtgg agggcgcatc attgctaata ttcagtgaaa tttattaccc ggaaggatgg
     1321 tcagccacta tcaacggtga gccacttcag attttacggg ccaactatgt tttgcgagcg
     1381 gcagtagtgc cggctggcga tcatgttatc aaatttacat ttgccccagg atcttattcg
     1441 aaaggtaata aagtcatgct tattttttcc atcctgataa tttgtttttt cttttacggt
     1501 ttgtattcag aatacaaaga aagtaaaaag gttgagttga aaaaattata acgtgattca
     1561 tcaagctgct aaatatcaaa aaccattgaa gtgaactcat acaaagaaag acgatacaca
     1621 aaaacaattt cgtttctcga aaaagttgcc ccgcctccgt taagaatcct ggacctgggt
     1681 accgaaaatc catttagcga gattatgaaa caaaaaggat acgaggtctg taacacaaat
     1741 ggtgaagatc ttgatgttga gtttgatgtg gtgaatcatt atagggttga catggtagca
     1801 gctttcgaaa tttttgaaca tatgctggcg ccttttaata tccttaaaga aatcaagtgc
     1861 agccgaatgg tagcatccgt gccgcttaag ctgtggtttg ccagcgctta ttggaatgaa
     1921 aaagatgact gggataagca ctaccatgaa tttgaaccaa agcaatttga tttcctgctc
     1981 agaaagaccg gatggaaaat tgttgcctct gaaaaatgga cctcagcaaa tccctggaag
     2041 tttggaatac gaccatttct aagaaatttt gttccaaggt attatattgt ttattgcgaa
     2101 aaaatctgat gggaattgta atcaggcaaa gcttttataa ctcaattttt tcctacctgg
     2161 gagtaatcat tggatttatt aatgttatct tccttttccc cgcttttcta tccgaagtag
     2221 atattggatt ataccgcgcc atacaaaatg cttctttact gctattgccc tttgcaactt
     2281 ttggacttca ggccagcacc ctgaaatttt ttcccgaagc ccaacagaag gaaagtacgc
     2341 aggatcaatt ccttggattt atgcttgtag cggtaacctt tttttatctg atttttttgg
     2401 ccttctattt cctctttcaa tcggttattc atcaatattt tgccgaaaaa gcacagccac
     2461 tgatagattc gttcaaccta gtattaatac tcgtatttat tttatctttc caaactgtta
     2521 ttgaagccta tagccggaat ttactcaaaa ctatattccc cggctttctg agagattttt
     2581 tctttaggtt tttgatttct gtctctgtct tgctttttgc aataaacctg atttcatttg
     2641 aggctctgct gaattcatta attggaatct atttgatagt acttctacta ttattgctgt
     2701 acttgtacag agatacaaat tttcagatcc ggcttccaac aacgaaagca ttcggctccc
     2761 tgggaaaaag aatcctcggt tatagtttct ttattttcct tggtttctcc ggttcaacca
     2821 taatagtgaa tgtagacagt atcatgatca gctcgatgct tggtctcgct caaacaggga
     2881 tttatgttac tgctatgtac atggggaata ttatcgagct tccaaagcga tccattggtc
     2941 aaataaccgc gcctctgctg gcaaaagcct ttgaagaaaa aaatttaaaa gaagtagact
     3001 cactttataa aaaagtatca attaaccagt ctgttattgg aacttattta ttgctgggaa
     3061 ttttagttaa cctcgacaac attttctcaa ttatgccaaa ttcagaggtt tttgctgcgg
     3121 gcagatacgt tgttttattt attgggttag gaaaattgat tgatatgatt ttcagcttca
     3181 acggagaaat aatcttattt tctcacttct ataaattcaa tgttacggca accatcatcc
     3241 tgggagtttt gaccattgca acaaatttgt tattgattcc cgtttatgga attagcggcg
     3301 cggcatttgc ctccttgctt accttattct tattcaatat cattaagttt gttttcctta
     3361 agattaaatt aaacatgcag ccctttaatt tcaacacact caaagtaatt gcattcggat
     3421 tgattaccta ttatttggtt tccattatac cacaattacc tgtatggata gacctgttga
     3481 ttcgatctat tttagttaca gttatttttg gtagcctgat tttgtttttc aaatgctcgg
     3541 aagaaatttc aaacctgagc cagggcggtt ggaaacaact tttcaagaaa aattaatgca
     3601 ggaacgtaac aacctcatct gctattattt tggctgaatt cccattgcca tataagtgac
     3661 tttggtgatt ccattcccgg gaacctaaat tttcataaaa agtttccaac tgatctaatt
     3721 tgccacctac aaggaaatta aaaccaccct caactagttc aacccattcg gtttcgttgc
     3781 gaagcgtgag gcaaaattta ccggcaaagt aagattcctt ttgaagtcct ccgctatcag
     3841 tcaaaaccat ttcacaattt tccagcagtt tgatcatatc aagataacca accggatcga
     3901 ttagctctat tccattttca acttcaatca attccatttt ttttcgtgtc ctgggatggc
     3961 acgggaaaac tactcttatc gatctgctca actttccaat cgcgtggagc aactgtgaaa
     4021 gagtttttgt gtcgtctaca ttggactgcc gatgaagggt aagcagcaca taattacctt
     4081 tctcaacgat cccatcaatg gatgaagata ccctctccag agatttttgt ctataaaaca
     4141 taaaagcatc taacatcaca tctcctgaaa gcacaatttt ttgctcttca tgattaaaac
     4201 cttcccgtaa taaattggaa acagcatgct ttgtcggaca aaaaagtaaa tcactaacgt
     4261 ggtcggttag aatcctgttt gtttcttcgg gcatcgaccg gttgaaagat cgcaaaccag
     4321 cttcaacatg acttagcctg tttccacttt tccgggctgc aatcgcggct gcaagtgtag
     4381 aggtagtatc cccatacacc attaccatat caggccggat gtcattaaaa atttcctgca
     4441 accctaaaat catctccccg gtcatggcag cttcattcct ggaattaatt ccaaggtttt
     4501 gatccggctt tggaatttcc atctcttcga aaaagatttc agacatgttg ggatcaaagt
     4561 gctgacctgt atgaataata atctcactca tgccttccaa acttctaaga tgcctggata
     4621 caaccgctgc ctttataaat tgaggccttg ctcctactac cgttattatt ttcttcaccc
     4681 tgataaatga tttaattccc gcgccaattt ttgagtcagt tcccgacgag aatatttttc
     4741 gatatttgat gttgaaacct ggcggaagtt tcttttaaac tcaagataca ttttttccat
     4801 gtagccagaa atacctgata catcgtcaaa atcaaaaatg actccgctgt tggaatcaga
     4861 aattatctgc gccacgtccc cgtcagtatc accaattgcc aatatcggct ttccagctgc
     4921 catatattca aagattttac cgctaataat tcctttggag ttaggagttc tgttaattgg
     4981 aagcagaaaa atccatgcgt cttgttgaat gcgaacaact tcttcatgat ccacgtagtt
     5041 tattttctca acaaacgctt ctattcctgc acgcttaatt tcctgaattg ctgaatagtc
     5101 ggttttgccg actaatttaa tttttaattc acttttggct tctggtatct tttgtaagaa
     5161 attggcgaaa gcacgccaaa gagcaggatg acttctcgtt ttagaaagca aacccacatg
     5221 aacgaaagaa aagacattag atacactact aacacttttg taatcatcag aatcataccc
     5281 gttggttata actaccgtcc tttttgcacc aaggttttgt aattcatttg caagagtttc
     5341 tccaaccgtt accaccgtat ccgcagtaga aagtacatcc ttctccagtt ccctgtgtct
     5401 tttatccgcc cacttagtga ggtagagatc tttgtaataa tcaatgtttg tccagggatc
     5461 tctaaaatcg gcaacccatg taacgttcaa ttttctcttc aatcctaatc caatgaggtg
     5521 gaggctatgc ggagggcccg tagtaacaac aatgttaact ggattttcct ttaaataacc
     5581 ggcgagaaac ttaattgagg gtttcaccca aaagattctg ggatccggaa taaagaaatt
     5641 gcccctgacc cactgaaaca ttttttgaat tattccgctt tttccttctt tcagaaaact
     5701 taccgtttga gcatctttat tccctgtaat ttttttgtag agattgtatg gttcccggat
     5761 tggagttttg attatttcta agttttgagg aacatctttt tccaggcttt tatcaattac
     5821 cggaaattct ccctggtggg gagtatagat tatggactcc catgaaaagc ctcttaagta
     5881 tttggcgaat tttaaccagc gttgtactcc cgagcctcca cttggcggcc aataataagt
     5941 gattataagt acttttttca ttaattcaag tgctccctga tgtgttccaa aattaggtcg
     6001 tattcattag gttcaaacca ggtaatcccg gaatctttcc tgaaccaggt aaattgtctt
     6061 tttgcatagt tgcgactatt ctttttcata agtttcacag attcctccaa agtaattttg
     6121 ccttccagat attcaaatgc ttcattatat ccaactgtct gaagggaaga ctcattctta
     6181 tatttcatca tagcccgggc ctcatccatt agtccatcat caatcatgga atcaagtctt
     6241 tggtttaaag tatcatatag tacttcgcgc gacaacccaa ttccaatttt aattacttta
     6301 aatggtctga cttttttctt ccctgaccgt aattctgaaa aaggaagtcc tgaacttcgg
     6361 attacagcaa gcgctcgtat cacccggtgt ggattccttc tgtctacttc ttcaaaataa
     6421 aatggatctt ttttttccaa ttcatttagc atatctgata aaccattcgt ttcaaattcc
     6481 ttctggagtt cttttttaat ctcagatggc acttcaggca ggtcatcaaa tccctctaaa
     6541 aacgctttta tatatagacc acttccccca cagagaatgg caattttttt tctattcata
     6601 ataccagctg caattttcag tgcaaccctt tcaaaatcgc ctgcagtgaa tttttcctga
     6661 acagaaatat agtttataag atgatgctta actttcgaca attgctcatt agtcggtttg
     6721 gccgtcccga tatttagctc catataaatc tgccggctgt ctgcggagat aatttctgta
     6781 tcaaaatact gtgcaagctt gatcgccaaa tcagttttac ccactcctgt tggaccacaa
     6841 atcacaataa tgcaatgatt ttcactttcc attggccgtt tcgttaccat gcccgatatt
     6901 cagagatagt ccaatgcctt tgctcttaag tagtccaaaa atggccaata ttggctcaaa
     6961 atcaaatttg ttcgccttag cagaattaaa cttttaaaat aaataatata aaaacttgaa
     7021 aaatgacttg gtttttctga aattaacctt gcaagaattt aagaagcgta ttctgccaac
     7081 aagaaactaa accccaatct ctcaatggaa aagagaattc tggttgtcga tgacagttca
     7141 gtaatgcaaa atttggtcaa aaaaattttc gcgcaattgg attatgaggt tgtttccgca
     7201 aaaaacggca atgaagctct tgaaaagctg aagaaacaag actttaatgt aattttgatg
     7261 gatataaata tgcccgttat ggatggcatg gaatgcacta aacaaataag aaaactgaaa
     7321 aataagaaag gccagctgcc cattattgca attacgggaa attcgaagaa ctatgcatgg
     7381 gacgacttca aaaaggcggg aatgagcaag tatttgcaga aaccattgaa cttcgatgaa
     7441 ttggtagaaa ttgtgaatag ttacgttaaa gatgataata aagtatagcc aaagttttct
     7501 caataaacta gaagaccttt tctcagaaac tgattacagc cttcgatatg agaaaggtaa
     7561 ttttaaaccc ggacattgcc ttataaggga ttccagggtc gcggtgatta ataagtatta
     7621 tcccctggaa ggaaagataa atgccttggt agaacttata aggtccatag aaataagaac
     7681 tgatcgcttg agcgaaaaaa atcaaaaact gtttgaggag ttgtctcaaa tggaggccag
     7741 gatttgaaag tcaccttttt aggaacaggt acatcccagg gagttcccgt aattgcctgc
     7801 gaatgtgaag tttgccggtc tctggatttt cgtgataaca gaacccgctc atccgtcttg
     7861 attgaatata agggtgtttc aattttaatt gatgcaggtc ctgattttcg atttcagatg
     7921 ctgcgcgaaa gagttaaaag gattgatgcc gttttgttta cgcacgcgca taaagatcat
     7981 accgcaggtc tggatgatat ccgctccttc aacttcaggc aaaagtcggt aatcccaatt
     8041 tatggaaccc aggaagtatt gaatcagata aagcaggagt tcaggtatat ttttgatgat
     8101 aagtcctatc cggggatccc tcagttgtca ctaaatgtta tagatgacca atcgtttaat
     8161 attaagggat tggaggtaat tccgattccc gtaatgcatc acaaaatgcc ggtgctggga
     8221 ttccgggttg gtaatttcac ctatattacc gatgcaaatg acatccctcc cttaagcaga
     8281 agagatatag agggaacgta tacccttgtt ctaaatgcct tgcaaaagga acctcatatt
     8341 tctcatttca ccctggaaga ggctattcag gtttccaaag aattaaaaat tccacagaca
     8401 tatttaactc acataagtca tagactggga ctgcaccaaa aagtaagcga ggaaattccc
     8461 aaaggagttg cattgtctta tgatggactt caactaactt tttaagtgca ttttaactac
     8521 ttttttcttt ccaaacttgc caaaagctta aaccaacgat tggcaggagc aaaaattctg
     8581 gaatgcttta gccagaacaa gaatgaactc attataacaa ctttgcccgt taatgagatg
     8641 aagttctttc atattagaat tgatttaaac cccaattatt cttttgtaaa cttcccggaa
     8701 aatttttacc gttcaaaaag aaacagtatt tctctatttg aaaaaatcac agcggctgta
     8761 attgccggaa tcagggttat aaataatgac aggagcctga ttattgaatt taccaacacc
     8821 tactccttgc tgttaaaatt ccatggtaac cagtcaaatg taattttact ggaagacttt
     8881 cgggtagttt cgctatttag gaaaaagctt ttgaaagata aaactcttga atttgagtct
     8941 ttggaaaagc aaataaatta ctccaaaaat tattttctta cagctaaagc taaccttgat
     9001 gagtatccaa ccctgggccc ttttgttaag aaacggatta aaatgttatg gcagtcagca
     9061 aaaagcattg atgaacaatg gaaaatcttt cacaaaattc tccttgaact ggaaggcgag
     9121 aatatttaca tactacggga ggagggtaag ttaatgttgt cattaataaa gatgggcaca
     9181 gtgatttcaa ctcattcgga tccactaacg gcgttaaatg agttcgctgc tatttttacc
     9241 agggagaata attttaactt gaaaaagaaa catgcggaga atcttcttcg atacgaagaa
     9301 aaaatacata atcaatcgct tcaatccctg actacttctc ttcaaaaatt agcgcaacaa
     9361 aagaattata aggtatatgc agacctgtta atggccaacc ttcataaaat taatccatat
     9421 gattccgaag ttacattgag taacttcact gataattctg aaattactat tcctttaaaa
     9481 atggggttga cccctcagaa aaacgcggag aactactaca gaaagagcaa gaaccagaat
     9541 attcaaataa cgaagaataa agaattggta aaactaaagg aaaaatccgt taaagagatt
     9601 cgaagttcgc tggaactgct taacgatgcg ggaacagtca aagagattga ggaaattata
     9661 gctcctttcg aaaaactgaa agcggacaaa tcaaaaagtc atccctacaa aacttttcat
     9721 ttcatggact ttgaaattag agttggcaag aatgcgaaat ccaatgattt aactaccttc
     9781 aaacactcct ataaagaaga tttgtggttg cacgtaaaag atgccggtgg ctctcacgta
     9841 atagtaaaat acaagtcagg aatgaatttt ccgagatcag taatcgagcg agcagctcag
     9901 cttgcggcat tcaattcaaa aaggaaaact gaaacattgt gcccggtgat ttatacgcct
     9961 aagaaatttg ttcgtaaaag aaaaggagat ccagcgggag ttgtggttgt ggagaaagag
    10021 aaagtgctgt tagttgaacc taaattctga ttatttaaca atatttatct taagcatgtt
    10081 tgtgcgttcc cttgcatgaa ttggcattga cgccatgtta atgaacacat caccctttga
    10141 taagtgaccc tcgcgaatga gaatttcttc aatatcagat atagtactgt ctgtcgattc
    10201 tgacttatcg taatagtacc ctcgaacgcc ccacaccagg cttaaagttc ttaacaaagg
    10261 cttgttggcg gtaaatgcaa atattgaagt tttcggtcgg taggaagcaa ccttatacac
    10321 ggtatagccg ctttgagtca ttccaaccat tgcttttgca ttggtttctt ttgcaagtac
    10381 aacggaagat gcaaccacac tgtcattcag gaaaatcggt gaattcttat ctgttggata
    10441 gaagcgaaaa taaagtctgt catgtgattc taccgagata attgttttta ccatactttg
    10501 aataactttc agaggatatt tgccggtagc agtttcggca gaaagcatta gtgcatcagc
    10561 accatccatt acagcattgg caatatcgtt tgtctcagcc cgcgtgggtt ttggattttc
    10621 aatcatgctt tccatcatct gagttgcaat tattacaggc ttggaagcaa tgttgcattt
    10681 ttcgactatc attttctgaa taagaggaac ttcttccata ggtgtttcta ccccaaggtc
    10741 tcctcttgct accataatag catccgctgt attgataatt tcatcaataa attcaagagc
    10801 ctccggcttt tcaatttttg caataatttt gatccacttt ccgcttttat tcaggatttc
    10861 tctcaaatca tgaatatctt ttggtgatcg tacaaaagaa agagccaccc aatcaacatc
    10921 atgttcaatt ccaaattgca ggtctttcag atccttggga gtcagcgaag gaaccagcac
    10981 ccgggtatct ggtaagttta tacccttttt ggattttaat tctccgccgt taagtaccaa
    11041 tgccgttaaa gattttccac ctgcctcagt taccttcaat tcaatttgcc catcattcaa
    11101 taatagttta tggcccggct gagccttatc aattaattcc gaaaattgtg tgtaaataac
    11161 ttcagaattt ccaatcccat ctcctgatct tatttttatt tctgatcctt cttccaggat
    11221 taccccaggt tccatttcac caacccttat ttttggtccc tgtaaatcct gcagcagaca
    11281 gatgttggtt ttcaattcct cattaatctc attcacgaac tgaattactt taaaatgatc
    11341 ttcatgtgat ccatgactaa aatttagtcg aataacatcc acaccctcct ttattaattc
    11401 tgtcagtttt gattttgagt ttgtagcagg tccaacagtt gcaacaattt tggttttgtt
    11461 aaaaagtatt tcactcatag tcgaatacca ggttttcttt tgattttaga tttatcgggt
    11521 cgattagaga actgtattga attatcttga tattgcgcac tatttttgca atttcttcag
    11581 catcaaaaga atccgctcct tttattttca gaaagaaatc gaattcctgt aattccggaa
    11641 ttagcaacat ttggccagac tcttttgttt caatgctgcg attacgtaac agaaacaatc
    11701 cggcattttc tgttttataa taatagttaa caaccgttat ggcattgcca tcattaaacc
    11761 gtattatttg gtctttggct ttaattaatt ggacttccaa agtacggttc aattcccagg
    11821 caattctata acctttggcg tgggagatta gcccaacaag gtcaaaatca aataatggat
    11881 caacaactag tttgctttta cgcattttct tcctctaaat caaaaatagg caaaatattg
    11941 ggctttttaa ggtaccttgc cttcataaag gaatcgatta agattcagtg aaattgtttg
    12001 acattattcg gtattatgta tttctttgca gagattttta ttaattactt aaaggataaa
    12061 tcatgtctga gatcgcgcaa aaagtaaaaa gcatcattat tgataaattg ggagttgaag
    12121 aatctgaggt aactcctgag gctagtttca cgaacgattt gggagcagat tccctggata
    12181 ccgtggagct tatcatggaa tttgaaaaag agttcaacat agccatcccc gatgaccagg
    12241 ctgaaaacat ttctacagta ggtcaggcaa tctcctatct ggaagaaaac gtaaaacagt
    12301 aagtttgtat taatcctgaa tttaagaccc atggatttca aacgagttgt tgtcactggg
    12361 gtgggagcac tcactcctat tggaaatacg gctaatgatt tttggcaggg actttccaat
    12421 ggggttagtg gtgccgccag gataaccaag tttaatccgg aaaaattcag gacaaaattt
    12481 gcgtgcgaag taaaaggatt tgatcctaat aatttttttg aacgcaagga tgccagaaaa
    12541 atggacctat tcacacagta cgcccttgcc gtatgtagtg aagtcgttaa agactcaggc
    12601 cttgacttaa cctcaatgaa ccttgatcgc gccggcgtaa tatggggctc aggtattggt
    12661 ggtttggaaa cttttcagga agaggtaaaa tcctacgcga tgggtgacgg tacgccgaga
    12721 tttaatcctt tctttattcc taaaatgatt gccgacatca gcgcgggcca gatttccata
    12781 aaatatggtt tcagaggtcc caatttcgtt acagtttctg cctgcgcgtc agcttcaaat
    12841 gctatcattg atgcctttaa ttatctgcgg attggaaaag ccgatctatt tattactgga
    12901 ggaagtgaag ctgcagtaac ggaagcaggt atgggtggct tcaatgcgat gaaagccctt
    12961 tcggaacgaa atgatagccc tgaaaccgca tcccggccat tcgataaaga tcgtgatgga
    13021 tttgttttag gtgaaggagc cggcggaatt attttggaag aatatgaaca tgcaaaagca
    13081 agaggtgcaa aaatttatgc agaattaatt ggtggtggta tgtctgctga tgcccatcat
    13141 attacagctc cccatccgga aggattgggc gcctttaatg taatgattaa tgcattggaa
    13201 gatgctcaga tcaccgccga ccaggtagat tatattaatg ttcatggtac ttctactccc
    13261 ttaggggata tcagcgaaat aatggctatt gagaaagttt ttggtgaaag cgcttataaa
    13321 atgaatatca gttcaactaa atccatgact ggtcatttat tgggcgccgc cggagccata
    13381 gaggccgttg cttgtatttt ggctatcaaa tatcaaactg ttccccccac aatcaatcac
    13441 ttcacagatg atgaacagtt tgaccctaaa ctcaacctga catttaataa agtgcagcaa
    13501 cgaacaatca acgttgcttt aagtaataca tttggatttg gaggtcataa tacttcaatc
    13561 attttcaaaa gaatgaatga gtaattttcc gatattcgga actacaaaaa aatcgtcaga
    13621 ttacgacaga acacttgcca aagctattag ggcaatgatt ggcaagaagc ccagaaatct
    13681 tgatcttttc aaacttgcaa cattacatgt atccgccgcc aaagaaaatg ataacggagt
    13741 cagagaatct aatgaaagac tagagtttct gggtgattca gtgattgatc ttgtagtgac
    13801 agagttcttg ttcaaaaaat ttcccctcaa aaatgaaggt tttctaactg atatcagggc
    13861 tagaattgta aaccgggaaa ctttaaacaa ccttgcccgg aaattaggga ttgacaatct
    13921 aattcaaatg gataacagta ttaaaggtgt tatggctcac aaatcccttt tgggaaacac
    13981 acttgaagcc atattgggtg ccatctatct ggatcggggt taccctttta ctcgccaatt
    14041 catattgaaa aaaataattt ccactcattt cgatttagaa gaaatcatta aaaataatcc
    14101 gaatcaaaaa agtaaaatta tcgaatgggc tcaaaaagaa aacaaaaccc tcactttcaa
    14161 tcttgttgag actatagaaa gaaataaccg caaacaattt attgttaccc tggagattga
    14221 agggcgaatt ctgggaacag gtgaaggatt taccaaaaag aaagcggaac aagacgctgc
    14281 ccaaaaatct tgtgaaatct taaatttaga ttgactgatg aggaaattaa gagtcgcggg
    14341 cggaacggta aatcaaatac cactggattg ggataacaat ctgtttaaca tcagtgcctg
    14401 tattaaggaa gccaggacaa ataaggtaga tctcctgtgc cttcccgaat tatgcctgac
    14461 cggctatggt tgtgaagatt tattcctctc tgactgggtt tcgaaaaaat ctctccggaa
    14521 gcttgttagc cttttgccac aaacaaaggg aattggcgtt gtgattggac ttccgataag
    14581 aattgataaa attacttaca atacttcctg cttcatctat gatggtcaaa ttcagggttt
    14641 ctatgcaaaa caaaatttag ccaatgacgg aatttactac gagcacaggt ggttcaagcc
    14701 ctggaccccg ggaataattc acacaatcaa gattgaaaat caggaatatc ctgtgggtga
    14761 aattattttt gaattaaaag gaattaagct tggccttgaa atttgtgaag atatttgggt
    14821 tactgaacgc ccggcttgca gatatctcga aaaaggagtt gacttgattc ttaacagcag
    14881 cgcgagtcac ttttctatgg gaaaatcttt ggaacgtcag gaactggtaa ttaacagttc
    14941 taatcttttt aactgcgctt acgtttatgc taatttatta ggcaacgagt caggccagat
    15001 aatatttgat ggtgatatta tcttggctca aaaaggaaac ttattagcca aaggaaaaac
    15061 attcggattt gaaagttaca ggattctttc atttgatttt gattttgatg ggggctccac
    15121 gaaaaattta gacaaggtaa ttcaaaattg taatgtgcaa ttccttcaag gggaatcatt
    15181 ggctttgttt gattatctga gaaaaagccg cagcaagggg ttcgtgattt caatgagtgg
    15241 cggcgcggac agtgcattat gcgcagtatt ggtttatcaa accatcaaat ccgctgtgca
    15301 agagttgggt ttagccggat ttcttgatcg aattgaccgg caggatttgc aatctgtaaa
    15361 aaatttaccg gctaaaaatc aaattccggc attggcggag gaacttctca tcctcgcata
    15421 ccaggcaaca aacaattctt caacagaaac tttaaacgct gcgaaagaat tatcagccaa
    15481 cctgaattgt aaattactac attggaatat cgataagagt attgaagagt atacaagcat
    15541 agttgaagaa aaaattggca aaaggcttaa ttgggaagag catgatgtta cgctccaaaa
    15601 cattcaggca agagcgcgtt cccccattat ttggatgatt gccaattata aaaattattt
    15661 gttgcttgtt acttctaaca gaagtgaagg aagtgttggc tacgccacaa tggatggtga
    15721 cactagcgga agccttgctc caattgccgg cgtaagcaaa aaatttatca gagaatggct
    15781 tgaatgggct gagaaggaat tcgaattgcc gtcactgaaa acaattaaca acctgaaacc
    15841 cactgccgaa ctgcggccgc cggatcgaag acagactgat gaagacgatc ttatgcctta
    15901 cacattatta caagagattg aaagcatggc tgtgaaacgt aaactttcac ctgttgaagt
    15961 tttccaggca ttgaaaggaa cagaaaaact atctgaaaaa gaattagcgg attatgtcat
    16021 aaaattcttc agacttctgg gacacagcca atggaaaaga gaaagatttg cgccctcctt
    16081 ccatatcgat gattataaca tcaatccccg gagctggttc agatttccag tactttcctc
    16141 ctcctttaaa gatgaaatta aagaacttca agacttaata aacaagtcta attagagtat
    16201 tccttatatt tggcacttaa ttttcattat gtttcaacct tcttattttc tttggaatgc
    16261 cgaccctgaa attttcgaga ttttcgggcg acctatagtt tggtacggac ttctgtttgc
    16321 ctgcgcattc ctcctcagtc agcaagtgat gtatcatatt tataaaaagg aaggcaagag
    16381 aattgaagat gttgatactc ttacagtata catgatcata gctgtaattc ttggagcccg
    16441 cctcgggcat gttttctttt atgaaccaat gagatatttg aagaacccca tagatattat
    16501 aaaggtttgg gagggtggtc ttgccagtca tggtgctgct gtgggcatac tcaccgcatt
    16561 gtggatttat acccgttata gtattaaatg gagcggaacc aggttaaagg ttaagaaaca
    16621 aaagcgggaa aatcagagct ttctgcaagt tgtggataga attgttattg tggttgccct
    16681 aagtggatgt cttatccgat ttggaaactt tatgaactct gaaatagtag gattacccac
    16741 gcattccaat tttggggtaa tgtttatgcg gaacgttact gatgctattc aatctaataa
    16801 tccgggagtg gaatcggtgg agtattccaa aagagctgga gagccaactg aagaaggtta
    16861 tgagccaatt cgcatagaat tagtattcaa tcctgattac agtcataatg aagaacaagt
    16921 tgtgttgtac ctggacaaca cattaaataa tatacttggt aattctacgt atgtcaccaa
    16981 taatatccat gagccactga ttcataacct ggattatacc tatcgaagcc ggcctgatgg
    17041 gaagattgtt gcaaccattg atactttcgg aatttccaga catcccgcgc aactttatga
    17101 gtccatttca aatctcttaa ttttcattat gctttatttg atttggagaa agtggcaaat
    17161 aaatgctcct cccggtaaaa tttttggaat cttcctggtt gttctttttg gcttgcgatt
    17221 catatatgag tttatgaaag aaaatcaggt agactttgaa caaggccttg ccctgaatat
    17281 gggtcaatgg ctcagcatac cattagtctt aactggcgca ttcctcctgc ttcgtccaaa
    17341 atcaccatcg agataaataa tacttaattt tttgaagtta tatctgtttt caaaaacata
    17401 tatataattt tttacatgta ttttagtctt ttttaaatat gcaatacttc actttattca
    17461 tattcttatt tattactgct caattatatg gacagtcata ttttaatctt gacccgagaa
    17521 gtgaagggag ggcaaatacc ttttcagttg aaacgggagc aggatcctta tttgtaaatc
    17581 catcaggaag cgcgatgctt gaaggtttca ctgccatttt tgcaggccaa aatattgcag
    17641 gcctggaagg attacatacc tccggaatag ctattgggca acctacaaga tttggagtta
    17701 tcggagcaat ttttggaaat accggagacg aattactaaa ccattcgggg cttgccttga
    17761 actgggcaca gaaacttgga attgcccagt taggattaag aaccggaatt attaatactt
    17821 ccattgaagg gcaaaagtca aaaaattcat ttcagcttga cttttccgga atagtaaagc
    17881 tttcaccgat attaagtgtc ggtgcgggga tttctaacct caccaaatcg aaatacaccg
    17941 ggcaggagca aattccggtt actggaaact ttggacttga cattcatcct acgcgatcat
    18001 ttcatctgac aatggaaaca gaaaaagatc ttgccaaaaa acccgaatac aaattcggtt
    18061 gttcatattc tataaaagaa cgaatcgaaa tcttaaccgg cataaatatt cggcctctgc
    18121 ggcaatccct gggtttaggt tttaccttga ggaagtttat tgtacaatgg tcattaacaa
    18181 accttccagg attgggatca aagcaatcca tttctctgtg ttataaaaca tccaaaaaga
    18241 aagatgcgta attggttcac tcttttgtca atcattgttt gttccaatgc attcggccaa
    18301 tctgatgaaa ttgatataga aagaatcatc aacacgaatt caattctgga tgatgaggtg
    18361 acttcagaag agattaatgc actccttctt gatattctgt cttcaccgtt ggatctcaat
    18421 acggtgagtg aagaagtttt gtcgggaatt agttttctta gcaacgaaca aattcgcaat
    18481 ttcattgact atagaaacca atttggacca tttatttcca aatgggagct tcaacaaatt
    18541 gaaggatggg atgttgatgt ttgtaaaatg gttgcttcgg tgtcaattgt tgataataaa
    18601 aacaacggca aatttcttaa taattttggc agaaatgcag aattcataat aagagaatcc
    18661 cggctattgc aggaaagaat aggatttgcc gattctataa atcgctttga aggtgacaga
    18721 aataaactcc aattaagatt caagagctct aaccccggag attattctgt ttcattagcc
    18781 gtggaaaatg acccggggga aaagttcagg tttcgttcag gccggttagg atttgatcat
    18841 atttccggca atgtgtcgct atttaacagg ggttttatcc gcaaaattgt tttgggagat
    18901 ttccgcctgc agtttggcca gggattaatt tttggaagtt cctttggagg tcgcaagagt
    18961 tcggaaccca ttatgagttt gcgaaaacct aacaccggaa ttattccaag ttcctcgttt
    19021 agtgaaacag gttttttgcg aggtgtcgca gtttcactag ggaagaaagg attgaacctc
    19081 actgtatttg gttcaaggat aagcaaagat gcgacactca gatttgattc ccttaagtct
    19141 gtggaattta ttacttcttt taatgagacc ggcctgcacc gaaatggaaa agaagtcaaa
    19201 atcaacaaca cggttaatga aatattggca ggtggtgcaa ttgcgtttag aaaatcaaaa
    19261 ttggaaacgg ggcttcaata tcatgcgggg aaatattcat ctccggtaat gcttgattcc
    19321 aagccttatc aactttatag atttcagggg cgcagattcc atgccggagg aattttcctg
    19381 aattatggtt gggctaacta ttatttattc cacgagtcag cagtttcaaa tttcaatggt
    19441 ttctccacat taacaggaat tatcctggcc ttgtcaaaaa atatagatct gtctgtttta
    19501 ttcagacatt tttcgccagg ttatcaatct ccatatggaa agggttttgc agaaacttca
    19561 actctggata atgaaacggg attatactgg ggattaaagt atcatctatt aaagtttaat
    19621 atctcagcct atttcgatta cttcaaattt ccctggctca aatttggagt tgataatccc
    19681 tcggacggtt acgagtatat gattcgaatt gattacctaa gcaaacaaac acctttttat
    19741 ctattattaa agaatgagaa aaaatacgct ggaaaggaat tggattctga acccgccaac
    19801 attatttact cggaaagaac aaatcttact gctaattttc aatttattct tacggagcat
    19861 gtttcaggta acattcgacc cgcggcaaca tattctaata caaagggtgg aaaggcttca
    19921 ggagtcttat tgaaacagga tctgaagatt gaatataatc ccttcacttt aaactttgga
    19981 tatttcctat ttgacacttc gtcatttgaa ggaagactgt attcaaatga gccggatgtc
    20041 agattccaat cctcgttccc attatttaac ggagaaggga tgcggtattt ctttcttctt
    20101 aaaatcaagg tgcgaaaagc tttgaatttc tggattaagt attacctgac gagttataat
    20161 aatattgatg aaattggttc cggtcccgat ttaattaaag gaaataagat ttcagaattg
    20221 aagacgcaaa taatttattc attcaggtaa ccattcatgt ttgagatttt ttcatattta
    20281 gggcttatag ttggatgtgg cgcgttattt attactgcct taaagacctc aattctctta
    20341 agaattgttg actttcaaag tctttccaac tggaaggttc tgcgcggtct aatcatcacc
    20401 ttcatgctag gctatctgtt ttcccttggt gcaatcgcca gcagaccgat aacttttgtg
    20461 gaagtaataa ccgtaaccat tttcctggct gggtcatttt ttgttttgct ggttgttaat
    20521 tccgctttta gagatattca actaatccgg agttccagga atctgctgca ggaccggaac
    20581 tttgaacttc aaaagttaaa tatacagctc gacacctttc tctatagcac cgcccatgat
    20641 ctgcgagctc cactaaccac tatccttggt ttggtagatt tgggaaaaag ggaaagcagt
    20701 gtgacagaaa aagactatta cttttcaatg attaaaaacc gggtagacac catggatttc
    20761 ctgttgaaag aaatcattgg attcactaaa aacaaacatt tagaaattca tcaggaacct
    20821 gtgaatttcc agaaaatgat agcatcggta cttgccgaac ttgaacccgt taaaatgaaa
    20881 gatgtaactc tcgaattgaa tattgatttt gataatgaaa tttggattga ccatcaaaga
    20941 tttcgttcga ttatttataa ccttttatcc aattcgtttc agttctttga tccaaagcga
    21001 tcaaatgcct gggttaaggt taacgcttca ataaatggaa aggaattaag agtttccgtt
    21061 gttgacaatg gaattggtat tgataaaagg taccaggaaa aagtttttga aatgttttat
    21121 cgggcaagtc ataattccgc cggctcgggt cttggattat atataacgcg ggaaattgtt
    21181 gttacccttg gcgggactat tcatttaaac tccactcagg gtgtgggtac tgatgtcgca
    21241 atatgtttcc cctacccatt atctcctttt aagaattcac taactcaaac accgtaattt
    21301 ccggtgcaat tccaattctg cctgggtaac cgataaatcc gaagccccgg tttacatata
    21361 gcttctgatt tcccttttgg tataaatctg cccattgctt atatgcatat tgagccggac
    21421 tccacttgaa atttccaagt tctacgccga attgaaatcc gtgagtgtga ccagcaaaca
    21481 ttacgtcgat atcggaataa tccggcctga tttgggcatc ccaatgactt ggatcatgcg
    21541 agagtaataa ttttacgcta ctctcattta tgttctgagt ggctttaaca atgtcgccat
    21601 actttgtgaa tcggccggcg ccccaatttt caactccaat tattgagatt ttctccccgc
    21661 caagctccaa ggtacggttt tcattcaaca gtaaatccca accaagcaat ttgtgtgctt
    21721 caatcatatc caggaggttt tttcttttcg cgtctgcaga agcccagctt ctgtaatctc
    21781 cataatcatg atttccaagg atagaataag ttcccaaggg agctttcacc ttattgaaaa
    21841 tatcaaagta tttattcacc tcatcagtct gggtattcac aaggtctccg gtaaaaaata
    21901 ttaaatccgg cttctcaccc atcagcattt ctataccccc ggctacagcc cgcttattga
    21961 agaaacttcc tgcatgtata tccgaaattt gagcaagttt aatcccatgg aattcctttg
    22021 gcaaattggg tattaccagt tttcgtcgaa tgatccggta attataagct ccggaaacaa
    22081 taccgtaact cattattgcc aggggaacac ctccggcgat tatagctgct tttaccagaa
    22141 attcagatct tttaatcttc tttccctcca aatgcttctc ctctgattta tgaataacct
    22201 tatttgtcca ctttattatc cgaactatgt cgtcaaccat aaggaatggt agtgcaagga
    22261 tttttgggaa aatgtttagg aagagaaaaa cgaggaaaaa acggcgcatc ccgttcatga
    22321 ctccctcgtt gggcgcataa ttaaaaccga aaagtgaggc aattgcaatg aaagaaattg
    22381 cccaaaacaa aattatcgtg accttacgaa ttgcttcagg ccaacttctt cttatttttt
    22441 ttgttgaatc aaataaataa taatcaatca acaacaaaat cagggcaatc ccaaaaacca
    22501 tcaacaactt atccattaaa actctcagta tttagatatc ccaagttttg tgttcagttt
    22561 gtctgttaat aaatacatga caggcacaat aactaacgtc aggaaagtgg cgaaagttag
    22621 accaaaaata attgtccatg acatcggtcc ccagaagatt acattatccc cacccaaata
    22681 aaaatcagga tcataatttt gagcaaagtg gataaagtca atatttaaac caaccgccag
    22741 gggaatcaat cctaaaattg tagtgattgc ggtcaaaaga acaggtcgca accttgttct
    22801 tcctgcctga ataattgcat tcctaatttc ttccggcggc aatctttgat ccgtgcccag
    22861 ctcttcccgc tttcttttcc tggtcagttc aatgaagtcc atgagcacaa tagcattatt
    22921 caccacaatt ccggcaaggc ttataattcc aatcattgtc atgattacga tgaaatccat
    22981 ttggaatatg attaagccca gcaaaacacc aatggtactg aaaagtacag acgacataat
    23041 aatgaaggga attgagactt tattaaactg tgacactata atcaggaaga tcataaatac
    23101 cgccagcatc aatgccttgg aaaggaaggc aaattcttcc gcttgctttt cctgttcacc
    23161 cgtgaacgcg atagtatatc cggcagggac ttcaaaatcc tgaatcaaat ctttgatgtg
    23221 attattaatg tctgtgggat tatatccatc caacacattt gaactgatgg ttataactct
    23281 gtccaaatca attctatgga ttgaacctag agaggaagta aattcagtac tggcaacgga
    23341 agaaataggt acctgataaa ttttcccatt gttttgattt ctgtacgtaa cgctcttgtt
    23401 catcagaaca tccacatcat atctgtattt gtcttccagt cttaactgaa cttcataatc
    23461 atcttctccc tgtttaaact tcgagatttc tttgccaaac aaagcggtcc tgatttctga
    23521 tgctatactg ccggttgaaa gtccaaaccg ccgcgctttt tcccggtcaa tttcaacaat
    23581 aagttcaggc ttacccgact caagattagt tttcagtttt tcaattccag caattttcga
    23641 ttcattaatg aatgctttaa ggttttctga aatggcaatg agtgtaggat aatctctgcc
    23701 tgaaacctca atatttaccg gttttcctgt gggaggaccg gcagcatctt tatcgacagt
    23761 gatggttacg ccgggatagt tgctgatccc ttttctgatt tcagacatga tatcgcttgt
    23821 ttgtactccc tttcgaagtt tgtattccag gaaattcact gtaattctgc ctttgttggg
    23881 agtttgtcct gctccaattg aacccatatc attaggatca ctagcgccat tgcctacatt
    23941 ggaaataatt gattccacca tataatcata aggttccaca attttggtca cttttttttc
    24001 aatcattgag gtgaactcat tggttgtagt aatgtcagtc ccaactggaa attcaatgaa
    24061 gacgttcaca tataatggtt ggttaacagg gaaaaaaata acattgggtt gaaatacgcc
    24121 cattaagaaa aaagagaaaa taaacaaaaa gaaagttccc cctaaaaaag caaatggtct
    24181 tcttcctgct aaggcatatg taattatttt aacatataga ttttcgagcc gaggtaaaaa
    24241 tgttttttga aacctccttg acaaaggagt tagtacaaag agattcaggg gaagtaagag
    24301 ggcaaagaaa atcaaaagat ttcccagcca ggtgatattt actaaaagga aaagaatccc
    24361 caatacaagt gcgattgctg aggtccttaa acctttttta taattgatct tggttggatc
    24421 atccaatcgc attaatacgg atatcaatac ggggttgatt atcaacgcga caaaaagtga
    24481 ggaacctaaa gttattatta aggtgattgg aagatacttc atgaattcgc ccatgattcc
    24541 cggccagaaa gcaagaggga gaaatgctgc cagagtagtt gccgtagatg caataacggg
    24601 ccatgccact tctccaactc ccttaatagt tgcatccttg ggcgagtagc cttcctcaaa
    24661 catccggtaa acgttttcaa ccaccacaat tccattatcg actaacatac caagggccat
    24721 aatcaggctg aaaagtacca tcatatttat tgtaactccg acagcctgaa gaatcataaa
    24781 tgccataaac atagataagg gaatagctat tccgacgaac aacgcgtttc ttgttccaag
    24841 gaagaaaagc aatacaagta ctaccagaat tactccgaag atgatagagt tctccaggct
    24901 gtttacctgt tcccgggtct gatctgaaag atcattggta atcgaaattt taacaccggg
    24961 gggaaatacc tctgcctgag cttttgccag cacagcaaat atgttatcgg taaccagaag
    25021 caggttttct ccgcttctct ttacgacatc aagcattaca acgggattgc cattcaagcg
    25081 tgcataactt tccttgtctt tatagtcaaa ctcaacccgg gctacatctt tcaaataaac
    25141 aattctgtcg ttttcattgc tgatcactat gttcagcagc tcagaagggt ctttgtattc
    25201 acccagaacc ctaatgtttc gtctaactcc gttagcaaga atatttcctc cggacaaatt
    25261 cagattttcc gcagcaaccg cactttcaat atcctgaaat gagagtttac gagcctcaag
    25321 tttgtatgga tcggctatta tctttacttc tttttcatca actcctctta tgtcaacttt
    25381 agagatttca ggaaagcctt caatttcatc ttccaggtac tcaccaaatt tgtttagctc
    25441 ttccaggcta aaatctccgg aaagattaat attgagaatt ggaaattctg aaaaatcgag
    25501 ttcaaataca tcagggtctg cttgcaaatc gtctggcaat tcctgctttg ctttatctac
    25561 cgcatctttt actttaagta atgcatcctc aacatcagta tctgtcgtga attcaacaat
    25621 aacggttgaa taatcctgaa cggaggtaga cttaatatta tctacttcag caattgtatt
    25681 aatctccttt tccaatggcc tggttatgag actctccata tcctcaggtg agtttccagg
    25741 gtgaatcgtg ccaacataaa tagttggtat ttttacctcc ggaaatgctt ctttgggcat
    25801 actaatatag gctccaactc caaggacaac tattataaat gttagaacta ctaccgaagt
    25861 ccggttctta accgcaaagg tagttaggcc aaactctttg tctattttct tttggtttga
    25921 atacttagat tccatgctaa tttgctttta cttctttaac catcattcca tcggtaacct
    25981 cccggaatcc tttattgatc acaaatgcag tattacttag ccccgccaga atctccgatt
    26041 cattttgaaa tgattttcca atttcaatgt gaacccgtgt agccatagtt ccatcatttc
    26101 cattttgtac tacatacaca taatctcctt tattatcttt ttgaattatt tccgttggca
    26161 ccaccaatgc ttcggggttt tcataatcag tcaggctcac caaggcagtc agatttggct
    26221 ttattaaagc atccatattc aataatttga tctcaagcga aaatgttctg ttgctgggat
    26281 caatcatctg accgactgcc gtaatcttag actcaaattt tcggttcaat gaaggaaatt
    26341 caacctgtac gggatcgttt gctttgaact taccaatgtg agactcagaa atatccgctt
    26401 tcagatacat gtcttttaac gatacgattc tgacaaccgg cccacccatc tgagccatct
    26461 ctccctcttt tataaaaacc tcatctatat gcccggagaa aggcgcccga attacagatt
    26521 gtgcaagttg agacttagct gtctcaagtt tccgctccag actttcgtaa ttattcttgg
    26581 cttgcaaata ctggatttct gttccaatat tgttttgcca aaggttttct tgtctttcat
    26641 aaataacttt ggccaattcc atggaagttt tcagctcttc aatattattt ttcaggatct
    26701 cagcatcaac agttaataat acctgacctt ccgaaactgc atcgccttct ttcactttta
    26761 ttgaagtaat tcttcccata gattcactgc tgacaacgat atttttcctg gattctacaa
    26821 ttgcccgcgc ctgtatctgg tgaataaatt tcttctttac gacaggtatg gtgcttacga
    26881 gcgtataatt acttaaagta ccagcgaaat caggatctgc ctgaactatt tgccgttcca
    26941 gtgctttaac ttttgattcc aggtccacca attgcctgtt ataaatatcc agctgagctt
    27001 tcattgtctc cagctcgctt tttttctctg tttggccgca acttgctgac acaaataata
    27061 agaaaatgat tgatagtgat gatattgctt tcattttatg gttaattcag tcttcctaat
    27121 gctttctgat aatcaatttt tgccaccaat gcctcataca aagcattgta gtaatttgtt
    27181 tgtgaagttt tataagagtt ttctgcctca attacttcca ggttagatcc taaaccttgc
    27241 tgatatttag ccctggagat ttcaaatact tcttcagcca gccccatatt ttccttctgg
    27301 gtgctcattc tggcaacgct attctccaga gaaatcctgg ccgtacgtat ttccatatca
    27361 atggctttct ccagccgctg tttttgattc tcccgctggt taataataat ttttcttttt
    27421 tgcatttgat tgctctttct gaagccatca aaaaccggaa cactaaggct gaagccgtat
    27481 aatccgtaag tgaaccaatt atctccgacc gcaaataaat cgccccagga tcctgttccc
    27541 gcatttgctc cgaaagttgc aaatccatca agcttaggta aataacccag gcgaatattc
    27601 tttagatcca tgtgagctaa ctccaagcct gtattcaagg ttgaatattc aatccggtct
    27661 tcataggtga actctgaaag atcctcagca ttcaaatcaa aggttatttg acttaaatct
    27721 tcagttaaga ccagttcctc attgtcaggc attgacacct ggaatttgag aacaagataa
    27781 ctggctataa gcgatctttc catattttcc atttccgttt tcagattatt gtgttcaacc
    27841 ttaagcctgt taatatctat tttctccgca aagccatttt caaaaaggag ttgagtctcc
    27901 ttcagcaatg tatcaatccg ctgataatta tttttgatca actcaagacc ttccttaaaa
    27961 actaatactg agtaatacgc tttgctcaca tccgctttca ggtctatttt tgatttgatg
    28021 aaatctttgg cagaaagctg ggtgaatgtt cgggctgctt tcaggccaag aaaataagcg
    28081 ccatccaaaa tcagctgcga aaatgtgacc gaagctccgc cattatattg agtctggaat
    28141 tgtacaggaa cgaaatcatc gtctccggca ttgggatcaa aaaatatcgc cggaataaac
    28201 gaagtttgaa ccctgaaatt atttatcaaa ttaatttcac cgcttatctg gggtaatcca
    28261 tcggataaaa cctcgccaac aaaagtctct tgtgcactgg cttcaaggcc cgcattcaaa
    28321 tattcttcgt tatgctttaa agcgtactcg atacactctt ccagtgtaaa acggttgata
    28381 attttttctt gtggaatggc cttaaggggt agcaagaatc ccaccatcag taaagttagt
    28441 ctcatagaac agaatttttt atttggtatt tttcatataa cgcttttcct tttgaagtaa
    28501 ctattccgaa aaggaaatga tcaaaaactt gcatttgcac ttctttaaaa tcaaattgat
    28561 cttttgggaa tactttttga tcaaaacaaa tttgcacctg ctcaactctg agccttgcca
    28621 ggatatttat gttgatatca tctctgaaat tcccttcttt cacgccgcgt gttaaattgt
    28681 ctgtaatcat ctggaagatg aattcatttt tatattttaa ataatattcc caggcatcag
    28741 gatgatactt attcaaatca aacaatagtg atggattcat ttcgtcaaac attctgcgaa
    28801 gacaaccgga aactttggaa agctcttcaa tggcattggc ggaactttct ttcatttcca
    28861 gaaacactcc cctgtctttt tcaataatcg ccatagtggc ctgcgataca acatcatctt
    28921 tatccttgaa atactgataa agggttttct ttgatattgc caaattgttc gctatgtcat
    28981 ccatggagac gcttcttatt ccgtaacgca caaacaaatt ccccgcgcct tctattattt
    29041 tctctttcat attctcatgc ggtaaaatta aacgagcgca aagctatgga aactctaagc
    29101 ctattccaag tttccatagt tttaatagtt cctaacgttt cggtcgttta ttgggttgtc
    29161 tttccctcgg aaaacatgac gaatgggggt tttactggga caatggttca aaattaaatt
    29221 acccatacag tgaatggaga aggtcagaat ccaccgaatg acttactttt gcgctgaaaa
    29281 gaggccttaa atgatcattt tttttaccac aaccaggaag gttacctatg cagtccaatc
    29341 cggttcccag ctctctaaaa atgatttaac caaacttgaa tggcttttca gcgccagata
    29401 tatagcggat aaccagctgg atacagatta cataggtccc cggaaagaaa tggtaacacc
    29461 ctggagcacc aatgcagttg aaattaccca aaacatggga gtacctggaa taaccagaat
    29521 tgaagaattt catcttgctg ataataaaac ggaatatgac cgaatgcttc agcacttcta
    29581 cggagagctg aaccaggaaa tattccgtat aaacagagct gccgaagcaa cgaaagagat
    29641 tgaggatatt ggagcttaca atgtatcgca aggcttagca ctaaacgatg atgaagtcac
    29701 ttatcttgaa ttggttggca aagaaattgg caggaatcta accgatagtg aagtttttgg
    29761 attttcacaa gtaaattctg agcactgccg ccataagatt tttaatggcc ggttcatcat
    29821 agatggagag acaatgcccg agtctttgtt tgaactcatt aaaaagacat ctgcacaaaa
    29881 tccaaacagg atcgtttctg cttacaaaga caatgtcgca tttgttaaag gaccagaagt
    29941 tgaacaattt tctcctggcc gtcaggatat acctgacact ttcaagataa atacctttga
    30001 ttctactctt tctcttaaag cggagactca taattttcca acaacggttg aacctttcaa
    30061 tggagctgct acaggttcgg gtggtgaaat ccgggacaga atggctggcg gcaaaggtag
    30121 tgttcctctg gccggaacag cagtttacat gacttcttat cccagattgg cggaatcaca
    30181 tttgtgggag aagaaaacag aagcacgaaa atggctgtat caacgtccac tggatatttt
    30241 gataaaagca tcaaacggcg ccagtgattt tggaaataaa ttcggccaac cccttatttg
    30301 tggtagtatt ctgaccttcg agttcataaa tgattcagtc agtttcggtt atgacaaggt
    30361 aattatgctt gccggcggaa tcggtttcgg aaagctaaaa gatacccaaa aagatattcc
    30421 ggcaaaagga gacaaggtga ttgtgatggg tggtgataat taccggattg gaatgggcgg
    30481 cagtgcggtg tcttccgtag ctacgggcga atatggagac gcaatagaat tgaatgcgat
    30541 tcagcgctca aaccctgaaa tgcagaaaag agtgtttaat gctatccggt ctcttacaga
    30601 aatggctgtt aatccagtta tttcaataca tgaccacggg gcaggaggtc atttgaattg
    30661 tctcactgaa ttattggaat ccactggggg agttattgac atttcaaaac tgccgctagg
    30721 tgatccaact ttatccgaca aagaaattat tggaaatgag tcccaggaaa gaatgggact
    30781 cctgattaaa gaaaaagatc tcgctacatt ttccaaaatt gccgaaaggg aaaaagcccc
    30841 ttattatatt gttggtgaag cgacgggaga ccatattttc aaatttatta acggacgaaa
    30901 tggaagcaat ccaattgatt taaaggtgaa ccatttcttc ggctcctcac caaagacaat
    30961 tttagaggat aaggatgcac aaatcgagta cgataaacta ttctatgata ttcaggcggt
    31021 ggaaacttac ttgcaggatg tattgagact ggagtcagtc gcatgcaaag actggctaac
    31081 taacaaagtg gatagatgcg ttaccggaaa agtttctcaa caacagacaa tcggaccggt
    31141 tcagcttcca ttaagcgacg tatcagttat ggctttggat taccgcggtg aaaaaggagt
    31201 tgcgacttct attgggcatg ctcctgtctc cggattaatt gatccagctg cggggtctgt
    31261 gctttcagcg gccgaggcgt taaccaatct ggtctgggct cccatagaag gtggtttaaa
    31321 aggagtttcc ctaagcgcaa attggatgtg gccggccaaa aatcccggag agaatgcaag
    31381 gctgtataaa gcagtgaagg ctttgagtga ttttgtctgt gatttgggca taaatatccc
    31441 aacggggaaa gattccctct ccatgtcgca aaaataccct gatgggaaaa aagttctgtc
    31501 tcccggaacc gtcataatct caactatagg agaagttatt gacataaaaa aggtaattac
    31561 tcctttgctt cagaaagtgg agtcggagat aatttacata gatttttcag gagttgaatt
    31621 caaattgggc ggaagcagct ttatgcaatg ccttgggcaa ttaggtggtg aaacgcccac
    31681 agtaaacagt gcagaatact ttgcaaggtg ttttgatgct attcaggaat taatgtcgga
    31741 agatttgatt cttgccggac atgatatctc ttccgggggt attattactt gccttttgga
    31801 aatgtgtttc acacgaaatg atatcggaat aaaagcaaat tttgagtttg aaggcctgga
    31861 tgcgatccaa attctttttt ctgaaaatcc aggcgttctt attcaggttg aaaatgcgaa
    31921 gaaagctgaa aaaaaattga agaaggctgg agtaaaatct cttaaagtgg gcgagataat
    31981 ttttgagtcc catcttcagc ttaaagtaaa tgagtttgat tacaaactgg atgttcgcaa
    32041 acttagaaat gcatggtttg agaccagtca tctttttgat ttacatcaaa ccaggaaaga
    32101 gtttgccctc cggagactgg ctaattatgg gcagcaagaa ttaagctata tactgccgtc
    32161 cgattggcag ggaacctatg aaagtttgga tattaagcca ttcagaaaag agcgaactgg
    32221 aattaaagct gcgattatca gggagaaggg agttaatggt gatagggaaa tggcctgggc
    32281 gcttcatatg gcaggttttg acgtactcga tgtacacatg acggatctca tggaaggaag
    32341 agaaaatctt gaagatatca gtttcctggt ttttgttgga ggtttttcca attctgatgt
    32401 gcttggatct gccaaaggtt gggcgggatc atttatgttc aatgcaaacg ctaagcaggc
    32461 tcttgataat ttttatgaaa gagaagacac attgagtctt ggagtttgta acggatgtca
    32521 acttatgatg gaattgcgat tgttgtatcc tgaaatgaat ccacaccctg taatgaaaca
    32581 caatgcggga ggaaagttcg agtgtcattt tctttccata gacgttccgg aaaataattc
    32641 cataatgttt aaatctcttg gcgggaccag gcttggcata tggatggccc atggtgaagg
    32701 tcgattcgag ttaccaggaa aagaagaaga ttacaatatt gcagccaggt atactttcgg
    32761 ggaatttcct ggcaacccca atggttctga ctttgctgtg gctgcattat cttcaaaaga
    32821 tggtcgacat ttagccataa tgccacacct tgaacgatca ttatttccat ggaattgggc
    32881 ttattatcat gagagcagaa aaactgatca agtttcacct tggcttgaag cttttataaa
    32941 tgtccgtaac tgggttaaaa gtaaggtttc aatctaatat tattctcatt cccgaattcg
    33001 gacacctggc aaaatcgtac tgcgttatct acttccttta cttttgatca aaaaactgct
    33061 attattatat ggtttttgtg tttgaacatc caatttgaag ggcctatgag aaaatttttc
    33121 ctgcctttag cacttcttat ttcagcttgc tcagtttatg aagaattacc ttcagactat
    33181 gattacagct ttaagggtaa gtttcgcacc tacgatacct ttgctttttt tcaagaacaa
    33241 tcggcccagg atgatggtac attgttcacc aaagagtcca ttgaaaatgt gatcgctgcc
    33301 aggatgagat cacaaggata cagccagagg gacaagaatc ccaacctttt ggtaatgtat
    33361 aaaatctttt atgacagtct ccgccttacg ggttataagc aaccagaact ggaagaatgg
    33421 ataaaatatg aagacgaaaa cattgaatac gttgagagaa aatatcagct gcgaatgggc
    33481 acactttata tcagtttctt tgatagaaag caaaagagaa ccgtttggca aggctatgcc
    33541 accaaggttt atggtaatga tatgtttacc aatgaacgac tgctcagaaa tgctgtcagg
    33601 tctgtactaa accggtacaa agtggtttct gaaagcttca ataagactat taaagaacgt
    33661 cggttttaag gggatt
//
LOCUS       DASWHX010000096         5265 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_3395023, whole genome shotgun sequence.
ACCESSION   DASWHX010000096 DASWHX010000000
VERSION     DASWHX010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 5265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5265
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3395023"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2693)
                     /locus_tag="VGA21_06500"
     CDS             complement(<1..2693)
                     /locus_tag="VGA21_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009580184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HEX9650191.1"
                     /translation="MEFRKINNITGWVVFGIAAIIYTLTVERTASYWDCGEFIAVSYK
                     LMVPHPPGAPLFLLIGRMFSFLAMGDVTQVAYWINMISVLSSAFSILFLFWTITLLGR
                     KLLGVTEKTVDENQKWILIAAGFIGAMACTFSDSYWFSAVEAEVYGMSAFFTAFVIWA
                     VLKWELIEDESRSNRWLILTAYMVGLSIGVHLLNLVTIPALAFIYYFKKFTKVTRWGI
                     IGTLGVSGIIILVIMVGIIPGLPSVAGGFELFFINSLGMPFGSGLIIFVLLLIAGLAY
                     SIHYSQSRGKVLMNTILLSLTFVLIGYSSYSLVLIRSNFDPPIDENNPENIMSFVSYL
                     KREQYGNRPLFHGQYFDARLTDQKQGAPVYTPGEEKYEVIDHKIEQVYDPNRTTIFPR
                     AYSSEPNHIREYRRIMNLGSGEVPTFADNIEFMIKHQMGHMYWRYFMWNFSGRESDIQ
                     DAGWLSPIDAMSDVPPSIAQNRGRNNYLMLPLLLGFVGLLFQYRKDPKSFSVVILLFI
                     LTGLALTVYLNSPPVEPRERDYIYPGSFYAFSIWIGLGVISLYAVFANFISNRKTAIA
                     AVFLVSLSVPIIMLQQNWDDHDRSDRYFSVDSAKNFLASCAYNAILFTGGDNDTFPLW
                     YSQDVEGFRTDTRVVVLSYFNTDWYIEQMTRQMYESEPFPFSLSEKDYRQGSPNDFVY
                     YVENPNVKGAIDLEQYISLIKQNHPALRVSASVSFYNTVPSRILSLKVDKEAVLKMGI
                     IPKGMEDLIVDRMQIEVKGQGLEKKDLMILDLIATNKWERPIYLNNTSKASIKIDLSP
                     YLIQEGNAYRLLPIENPNPGADFVNTEIMYDNLMNKFFYRELDNPNTYYNEDYRSFVL
                     NHRSSFNTLAEALVNEGNEQRAREVIFKSLELMPDAAIPYD"
     gene            complement(2762..3388)
                     /locus_tag="VGA21_06505"
     CDS             complement(2762..3388)
                     /locus_tag="VGA21_06505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650192.1"
                     /translation="MKNFISSLLETNGKFLFLFVCLTTFLLLLMKKNFIEAEIAAFEV
                     LEGQGHGGVISLMNALQYFSIPIIYFWKFTIISFMLWIGCFTFGFKINYNTLWKVAMI
                     SELVFFLPELIKIFYFILIFPDPNLHEVRAFYPFSLMSLFDYEALSPAWHYPLKALNI
                     FELVYWFVLIQSLKLTIKKDPVQIRWLAMSFYTGPFILWLCYYVIIYK"
     gene            3504..4721
                     /locus_tag="VGA21_06510"
     CDS             3504..4721
                     /locus_tag="VGA21_06510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HEX9650193.1"
                     /translation="MKFSVVLGMIAIPLLGFGQNLVTAEHKIDSASVIYVDGVAQKML
                     LENPKVQIESTQALNDMYNFKFEIAERQFRWIIQRYPWHPIGYFMQALGEWWKIMPEL
                     EDESLDENFYAYIDTTIKVAETLHLNKEYQVEAAFFLAAAYGFKGRLLSERSNWTKAA
                     VAGKNALNYMDETRYKGGLSPELLFGYGLYNYYAEWIPENYPALKPILWFFPNGDKEL
                     GVKQLNEVTRNALYTRVEASVFLSRILYSDNINREESMQITEYLATQYPDNPYFSRSY
                     ARQLYSTGKYRDAEKLCKEILTKVESRQQGFGPKTGRYISFFLGQISESGGNEEDAQN
                     YYEKTLEYGNLEPGGQEGGYYLFAAYKLAIFADLRGDDLAFENYFGLVKKNSNRKDAV
                     WKNMKEYKRKKNS"
BASE COUNT         1726 a         1044 c         1080 g         1415 t
ORIGIN      
        1 aaatcataag gaatggcggc atccggcatt aactccagac ttttaaaaat tacttcacgt
       61 gcccgctgtt cattaccttc attgaccaac gcttcagcca gcgtattgaa acttgacctg
      121 tgattcaaaa caaaacttct atagtcttca ttataatagg tattcggatt atctaactcc
      181 ctgtagaaaa atttgttcat caaattgtca tacataattt ccgtattcac aaaatcagct
      241 ccgggatttg ggttttcgat aggcagaagt ctgtaggcat ttccttcctg aataaggtat
      301 ggagaaagat cgattttgat acttgccttg gaagtgttgt taaggtaaat aggcctttcc
      361 catttgttgg tagcaatcag atcaagaatc ataagatctt tcttctccag tccctggcct
      421 tttacttcaa tttgcatccg gtctacaatc aaatcttcca ttcctttggg aataataccc
      481 atttttaaaa cagcttcctt gtcaactttc aacgacaaaa tgcggctggg aactgtatta
      541 tagaaactta cggatgccga cactcttaac gcgggatggt tctgtttaat tagcgaaata
      601 tattgttcaa gatcaattgc gccttttaca tttgggtttt caacataata aacaaaatca
      661 ttggggctgc cctggcggta atctttctcg ctcagcgaga atggaaatgg ttccgactca
      721 tacatttgcc gggtcatttg ctcaatgtac caatcagtat taaagtagct gagtactact
      781 accctggtgt cggttctaaa tccttccaca tcctgcgaat accacaaagg gaaggtatca
      841 ttatcgccac ctgtaaatag aatcgcgtta taggcacagg aagcaaggaa attttttgcg
      901 gaatctacgg aaaaataccg gtcacttctg tcatgatcgt cccagttttg ttggagcata
      961 ataattggaa ctgaaagaga gacaagaaat actgccgcga ttgctgtttt ccggtttgag
     1021 atgaaattgg caaaaacggc atataaacta atgacaccca gacctatcca aatagaaaaa
     1081 gcataaaaag aacctgggta aatgtaatct ctttcacgag gctcaaccgg tggcgaattc
     1141 agataaactg tcaaagccaa ccctgtcaaa ataaataata aaatgaccac actgaaactt
     1201 tttggatctt ttcgatactg aaagagaagt ccaacaaagc caagcaacaa ggggagcatt
     1261 agataattat ttcttccgcg attttgtgca atgcttggcg gcacatcact catagcgtca
     1321 ataggggaaa gccaaccggc gtcctgaata tcactttccc ggccggagaa attccacatg
     1381 aaatatcgcc aatacatgtg tcccatctga tgcttgatca taaactctat attgtcagca
     1441 aatgtgggaa cttcaccaga tcccaggttc atgatacgcc tgtattccct gatgtgattg
     1501 ggttcgctgg aataagcacg tggaaaaata gtggtgcggt taggatcata aacctgttca
     1561 atcttatgat caattacttc atatttctct tccccagggg tataaaccgg cgcaccttgt
     1621 ttctgatcag taagccgagc atcaaaatac tgtccatgga ataaaggcct gtttccgtat
     1681 tgttcacgct tcagataaga aacaaagctc attatatttt ccggattgtt ttcatcgatt
     1741 ggagggtcaa agttcgatcg tatgagtacc agggaatagg aagaataccc aatcagcaca
     1801 aatgttaaac ttagcagtat ggtattcata agaaccttgc ctcttgattg agaataatga
     1861 atactgtaag ccaatccggc tattagtaat aaaacgaaaa ttatcaatcc ggaaccaaat
     1921 ggcatcccaa gcgagttgat gaagaatagt tcaaatcctc cggccacact ggggagtccc
     1981 ggtatgatgc ccaccataat caccaggatt atgattccgc taactcccaa agttcctata
     2041 ataccccaac gggtaacctt cgtaaatttc ttgaaataat aaataaaggc aagcgcggga
     2101 atggttacca aatttaataa gtgaactcca attgaaagtc caaccatata ggcagtcaga
     2161 ataagccaac ggttggaacg actttcatcc tcaattaatt cccactttaa tacagcccaa
     2221 ataacaaacg cagtaaaaaa ggctgacatt ccataaacct ccgcttcaac agcactgaac
     2281 cagtatgaat ccgagaaagt acacgccatg gcaccaataa atccagccgc gatcaagatc
     2341 catttttgat tttcgtcaac ggttttttca gtaacgccca aaagctttct tccgagaagg
     2401 gtaatggtcc agaataagaa aaggattgaa aaagcactgc taagaacact gatcatattg
     2461 atccaataag caacctgggt aacatctccc atagccaaaa aggaaaacat tctgccaatc
     2521 aaaagaaaca aaggcgcccc cggaggatgt ggaaccatta atttatatga aacggctata
     2581 aattcaccgc aatcccaata actggctgtt ctttcaaccg ttagggtata aataatggct
     2641 gcaataccaa aaacaaccca tccagttata ttattaatct tcctgaattc catgaataac
     2701 ttttagcagg cgaaaataag aattatttga cgaaaatcag gccaaaatga gctttttaac
     2761 gctatttgta gattataacg taatagcaaa gccataagat aaatggtccc gtataaaagg
     2821 acatagccag ccaccggatt tggaccggat ccttttttat ggttagcttc aggctttgaa
     2881 tgagtacaaa ccaatatacc aattcaaaaa tattcagggc tttgagggga tagtgccagg
     2941 cgggtgacag ggcttcatag tcaaacaaac tcattaatga aaatgggtag aatgccctta
     3001 cttcatgtag gtttggatca ggaaatatta aaatgaaata aaaaatctta atgagttcag
     3061 gaagaaaaaa taccaactct gaaatcatcg ccactttcca taatgtattg taattaattt
     3121 tgaacccaaa ggtgaaacat ccaatccata gcataaaaga gataatggtg aatttccaga
     3181 agtaaattat gggtatggaa aaatattgca aagcattcat gaggctgata accccgccat
     3241 gaccctgacc ctccagaact tcaaacgcgg caatttccgc ttcaatgaag ttcttcttca
     3301 ttagtaaaag cagaaaggtt gtaaggcaaa cgaaaaggaa aagaaatttt ccattcgttt
     3361 caagcagaga agaaatgaaa tttttcatgg agttataaaa ctacaagcgt ggtcagtgtt
     3421 tgaagactct aaataaagta atttggcaca taaaaatggg taatggcctg aattttgaat
     3481 aaccggtcat aaaaagcatg tatatgaagt tttctgtggt tcttggaatg attgctattc
     3541 cgcttctggg cttcggacag aatcttgtta ctgccgagca caagattgat tcagcctccg
     3601 taatttatgt ggatggcgta gctcagaaaa tgctgcttga aaacccaaaa gtgcagattg
     3661 aatctacgca ggcactcaat gacatgtata atttcaaatt tgaaattgct gaaagacaat
     3721 tcaggtggat cattcagaga tatccatggc acccgatagg gtactttatg caagccctgg
     3781 gggaatggtg gaaaataatg ccggagcttg aggatgaaag tctggatgaa aatttttatg
     3841 cttatatcga cacaacaatt aaggttgcag aaacattaca ccttaataaa gaataccagg
     3901 ttgaagccgc ttttttcctg gccgcagctt acggattcaa aggaaggctt ttgtcagaaa
     3961 gaagcaattg gactaaggct gcggttgccg ggaagaatgc ccttaattat atggatgaaa
     4021 cccggtataa agggggctta agtccagagt tgctttttgg atatggtctc tataactatt
     4081 atgccgaatg gattcctgag aattacccgg cactaaaacc aatactttgg ttttttccaa
     4141 atggagataa agagttaggt gtgaagcaac ttaatgaagt aacccgaaat gcattgtata
     4201 cccgcgttga agcatcggtc ttcttgagtc gaattttata tagcgataat attaacaggg
     4261 aagaatcaat gcagattaca gagtacctgg ctacgcaata tcctgacaac ccctacttca
     4321 gtcgctctta tgcaagacaa ctatattcaa cgggaaaata tcgtgacgct gaaaaactct
     4381 gcaaagaaat tctgaccaaa gtagaatctc gccaacaggg attcgggcca aagaccggca
     4441 gatatatctc ttttttcctt ggtcagatta gcgaatccgg aggcaacgag gaagatgctc
     4501 aaaattatta tgaaaagaca ttggaatatg gaaacctgga acctggcggg caagaaggcg
     4561 gctattattt atttgccgcg tataagctgg ccattttcgc ggacctgcgg ggcgacgacc
     4621 ttgcttttga gaattatttc ggcctggtaa aaaagaattc caaccggaag gatgctgtat
     4681 ggaaaaacat gaaagagtat aaaagaaaaa agaatagtta aagaaaaaat ggtaattgaa
     4741 gatgcgctgc tggagaaaag aagttttcct ggcaaatccg ttagtttctc tttgtgagaa
     4801 aaattttatg gagtcccgct gatatcgcag gtcaccgcag ggaaaaaatt gttcggggag
     4861 tcttctgcgc aaatccgcga aatctgcggg agaaaagaag ttttctgtga aaattccgtt
     4921 agttgagctt tgtgagagaa attttattga gtcccgctga tatcgcagat caccgcagaa
     4981 aaaattattc agagatttct gcgcaaatcc gcgaaatctg cgggagaaaa gaacttttcc
     5041 gcgaaaatcg gttagttgag ctttgtgaga caaattttat ggagtcccgc tgatatcgca
     5101 gatcaccgca gaaaaaatta ttcagagtct tctgcgcaaa tccgcgaaat ctgcgggaga
     5161 aaagaagttt tgcgcgcaaa tccgtaacta ttcataaagc acaaaaagcc gcccccggtg
     5221 tgggaacggc ttcaattgta cgaaacagaa ttgtgattta atttc
//
LOCUS       DASWHX010000097        18541 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_77929, whole genome shotgun sequence.
ACCESSION   DASWHX010000097 DASWHX010000000
VERSION     DASWHX010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 18541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18541
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77929"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            212..1819
                     /locus_tag="VGA21_06515"
     CDS             212..1819
                     /locus_tag="VGA21_06515"
                     /inference="COORDINATES: protein motif:HMM:NF013974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX9650194.1"
                     /translation="MIQLISGESAFAYGAINSGVRLITGYPGSPSSGAFELLRNLQIK
                     FGYHMEWSLNEKVACEVAIGGSIGGLRSLICTKSVGLNVMIDPLMALNLTPLNAGLVI
                     LLGDDPQGYGSQNDQDSRAIVHFLEMPLWEPAAPADGYFMMINAFQVSEEFGVPVIIR
                     ETRSFSQVTGEIDFELKYDPEKKELIEEPFRFVPVPENAVAKHLALQKSLSKVTIWHE
                     NSVFNETTGNGKKGILASGFVTHKLLDILEGAEKHPFRIFKLGTLFPLPYRKIEEFLN
                     DCDEILIFEESSDFIERAIKVIAWETHCATTILGKSELGLSPAGELFKWQISNALNTL
                     FPDLKIKVYLESAQAEEYPEMKNNCSDSRYEEVIELVERAAQLGGLTPLFVGDPGCIF
                     MAGEKLIAKYAIGSAVGLASGLQYANPDKPVVAFVGDSGFFHTTMPAIANAAFTLRNF
                     LIIVLDNGGALTTGLQPTPAGGKNILNQESMPQSFQKIAESLGIQQVSKISLNDEPDK
                     IINAFLAGLTGKKLNLIIVQIPSTTAG"
     gene            1925..2965
                     /gene="arsS"
                     /locus_tag="VGA21_06520"
     CDS             1925..2965
                     /gene="arsS"
                     /locus_tag="VGA21_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017730546.1"
                     /note="Some members of this family are selenoproteins;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenosugar biosynthesis radical SAM
                     (seleno)protein ArsS"
                     /protein_id="HEX9650195.1"
                     /translation="MKTLNRRQHKLSDPAFQVSVLSDPQSTGVVEFNEKLAGKGLLPL
                     KAYLPEILQINLGKMCNQTCRHCHVDAGPDRTEIMTRQTMQEILAKIPGSPISTLDLT
                     GGAPELNPEFRWFVDELMKFKKHIMVRSNLTIIVSNKKYHDLPEFFRHHDIEIIASLP
                     YFSANRTNAQRGEGVFEKSVQALKMLNKAGYGSENSNLKLNLVYNPTGAFLPGNQQGL
                     EREFKNRLQAEHGIVFNNLFTITNLPISRFLDYLISTGNLESYMHKLVNAFNPAASEK
                     LMCRNTLSIGWDGSLFDCDFNQMLDLSLNHGMNHIKDLNWRSLEGREIITNRHCFGCT
                     AGAGSSCGGALT"
     gene            3077..4489
                     /locus_tag="VGA21_06525"
     CDS             3077..4489
                     /locus_tag="VGA21_06525"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013455477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX9650196.1"
                     /translation="MIFLFSLGQLHLTWHYIRNKKRTTLSPGELSTYPKVTVQLPLYN
                     EKNVVERLINKIVQLRYPKELLQIQVLDDSTDETSLIVSKLVSKYKIEGYLIDHLVRN
                     ERVGFKAGALQNGLQKSTGEFIAIFDSDFLPPVNFLLKSLPHFQDKSVGIVQSRWGHL
                     NEDYSLLTQIQAFGLNAHFSIEQSGRKAAGSFINFNGTGGVWRKTCIEDAGGWSADTL
                     TEDLDLSYRAQLKNWKCEYLEDLISPAELPILISAIKTQQFRWNKGAAETGRKLIKQV
                     IGSSKRKINKLHSIMHLFNSSVFLFLFIAGVLSIPVLFFKQGNRNYEILFDAGAFFLI
                     GFFSITFFYRVAYKNIKPSSGKSFAFLFPVFLVVIMGLSLHNSVAVLQGLFGLKSPFK
                     RTPKFNVFRKGDEWKSKSYSEIKANKISWTEGLISLYFLAGLVAGIYLGDFKLMLFHC
                     LLFSGFTSVFYLSIKHQFAG"
     gene            4658..5071
                     /locus_tag="VGA21_06530"
     CDS             4658..5071
                     /locus_tag="VGA21_06530"
                     /inference="COORDINATES: protein motif:HMM:NF039704.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6252 family protein"
                     /protein_id="HEX9650197.1"
                     /translation="MGCQKETIMPGITGGSLKAEVNGNTFEATGILAFRTQPTSTTGE
                     GFYVSGNTTIGFPQITLTLYSSLQGKYQLGVSGSGNTQASYALSVDNQELSISGNINI
                     TTINYSEGGTVKGTFEFATATNSITNGSFEIRFSN"
     gene            5156..5540
                     /locus_tag="VGA21_06535"
                     /pseudo
     CDS             5156..5540
                     /locus_tag="VGA21_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009184287.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
     gene            5878..6270
                     /locus_tag="VGA21_06540"
     CDS             5878..6270
                     /locus_tag="VGA21_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019600275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin"
                     /protein_id="HEX9650198.1"
                     /translation="MELAMHPNELLILFNRDSAVAMKTLAFAKTVSNHIRDMEFRSLK
                     FPNTIWKELLNMLNLEAKDILDKSHPDYQKKIMGHDFSTEGWLNVLSHNSYLIKAPIA
                     VKNGKAVLCNRPKDVLKLINEEDKIPVS"
     gene            complement(6335..8743)
                     /locus_tag="VGA21_06545"
     CDS             complement(6335..8743)
                     /locus_tag="VGA21_06545"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX9650199.1"
                     /translation="MLKYSIMLFGFLLPEFAQSQQGIIFSGRITDESGYPVEHALVQI
                     KETQRTSITDVNGNFEIHNVPEEQFIILITHVSFLPFEEKINTQVKEDFLITLKENPH
                     VLPQVFVINKHNLLFSKIPGSVSYIDNNELDKLKPISGNEVFRRIPGLNVVDEEGAGL
                     RVNIGIRGLDPDRSRSVLIMEDGVPVALNPYGEPEMYYSPAIDRMNGVEVLKGSGQIL
                     YGPQTVGGVINYITAAPPEKEEIRLKLQGGKGTFLTALGSYGNTFNKTGVQIDYLHKR
                     ADDFGALNFTVNDFTAKVKFLLSERATFGIKLSAYDEVSNSTYVGLTQSMFETGGYDF
                     SVLMPNDELDVRRYLMSFSNNINLRKGINLQSMLYGYTTTRNWRRQGYSYNSYTATNP
                     NPPPSDWTGVVLGDESTDGGAIYLRDRTGNRNRQFEVIGWEQKILHNKSFGNFNNELI
                     AGYRILYERAYEQRINGTNDQAKSGDLISDEIRTGNALSLFALNKFSISQKLDITPGV
                     RVEFYSYEREILREANIDTNRVADNQIREVIPGFGVNYRPASKLNFFTGIHRGYAPPR
                     IKDAIDFSAVNPVLELEAEKSWNLEVGLRSQPIKGMFLEVTWFYMDFENQIIPSSQSI
                     GGPGFGVTNAGRTLHKGFETSFTLDSREFLTSNWKIQFDLSATTIKAIYNSDRLVSSG
                     NDQVNVRGNRLPYAPEFTLSCGFSIEAPFGTGLRLTDNYIGNQFTDELNTVNASFNGR
                     IGKIGSRNVLDATLYHIISSMNATINISIKNVTDERYIATRRPEGIRVGLPRYITAGF
                     AIKF"
     gene            8928..10169
                     /gene="hemA"
                     /locus_tag="VGA21_06550"
     CDS             8928..10169
                     /gene="hemA"
                     /locus_tag="VGA21_06550"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HEX9650200.1"
                     /translation="MKDAFMGINSTFFNRLENFVVIGSNFTKADINTRSRFAITGPHS
                     EKVYRKAVDMGFGDFLLLSTCNRTEFYSCGSVTTLRSLVKQQLLLTDEDLDTYFYTYS
                     GTDAIQHFFRVLSGLDSQIIGEYEIVNQVKSALKASRYYGLTGTISDRISNFALQASK
                     EIKSETNLSKGKYSVSYAAAELMLEKQKAQLVEKVLIVGTGDFGRTVARNFRQYFPDI
                     NLSLTNRTQKKAQQLAVQLNANILPFETFKKHLHTFDAVITTIGTEKYMINPGDIPLN
                     KPQLYLDLSVPQAINPRVDLITDVQLYSVDEISAFHNDLLAERMSEIPEAEEIIAQHI
                     DRLLAWNNVHKHRKIILGYKEKIQNLVSNQRDAHVNGNSRLINHKIDKSFSELIQKIK
                     THGYAGCIMIEAMDAFVPAEK"
     gene            10166..11086
                     /gene="hemC"
                     /locus_tag="VGA21_06555"
     CDS             10166..11086
                     /gene="hemC"
                     /locus_tag="VGA21_06555"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR00212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HEX9650201.1"
                     /translation="MTQSNIIRIGTRESALAMCQAREVQSKLHELSIPSEIIQVISDG
                     DVDLETPLYEMGIQGIFTRTLDNALLQKKVDIAVHSAKDIPTRLASGLCLAAVLERGS
                     EWDTLVQLHSSPAIDLTNTCTIATSSLRRKTQWLYRYPCHKIESLRGNIQTRLDKLNN
                     GSWQGAIFAQSALDRLKVNSNPVTILYWMLPAPAQGAIAMVCRIDDGLSREACNQINH
                     SESEICIKVEREFLAALHGGCAVPIAAFAKIHNGSMSFKGNILSLNGKQKCEVEMQFE
                     PDQTKDAGQVAAAEIIKRGGESILKTFRKV"
     gene            11083..11787
                     /locus_tag="VGA21_06560"
     CDS             11083..11787
                     /locus_tag="VGA21_06560"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HEX9650202.1"
                     /translation="MTRKLHIVSTRCLDSFNFQKLNEKEFTFSSVDFIRKKVDIPVNI
                     SKLSFHKDIVITSKMGVISFLQIARMKGIDLQKCSIYCLSHGTRKYAKNSGLKIVKTA
                     SNASNLAEEILNDPDVKAVTYICSNKRRNELSERLIKAEVIVQEVIAYRTDLTPAKIE
                     QPYDAILFFSPSAVESFLKLNPLKPAACFCLGETTADYALKHGYVDIHIPPIPTEESL
                     LETVINHFSIYPAHAM"
     gene            11777..12817
                     /gene="hemE"
                     /locus_tag="VGA21_06565"
     CDS             11777..12817
                     /gene="hemE"
                     /locus_tag="VGA21_06565"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013454060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HEX9650203.1"
                     /translation="MLCNDLLLRALRQENLIRPPVWMMRQAGRYLPQYIRLRKKYDFF
                     TRVQTPELACEITLQPVDVIGVDAAIIFSDILVIPQAMGMKVLMEEGKGPHLPNPIQR
                     LHDIEMLDIESISKQLNYVFEALKLTKQELNNRVPLIGFAGAPFTLLCYMVEGKGSKG
                     FEKAKAFCFTQPELAERLLNKITLATIDYLNGQVKAGADVIQIFDSWSGLLSPDDFDR
                     FSAPYLIRIAEALTQQVPIILFPRGSNYALKSLSQNYAVSGIGIDWTISSAQARELTQ
                     YNVALQGNFDPIKLLMPPAEIKKQVWKMIDEFGTKGYIANLGHGILPYIPVDHAKAFV
                     DAVKEYRYEPIN"
     gene            12801..13739
                     /gene="hemF"
                     /locus_tag="VGA21_06570"
     CDS             12801..13739
                     /gene="hemF"
                     /locus_tag="VGA21_06570"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF003727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HEX9650204.1"
                     /translation="MSQLTKEHFTSHIRTLQNTICEEIEKVDGKGTFNSDLWERAEGG
                     GGETRVISNGNVFEKGGVNISVVHGKLSQPAAKQLLINGDSFFACGLSLVIHPFNPFV
                     PTVHANWRYFEVYDDNSLLLDAWFGGGTDLTPYYLIESDVVHFHSVQKQNCDKFDTSL
                     YPVFKEQCDNYFSNHHRGGERRGVGGIFFDHRRETAGKSIEFWRKFTEANSLVFTDAY
                     LPIVLKRRNTHFTSEHKFWQEIRRGRYVEFNLLHDRGTLFGIRTNGRSESILMSLPPV
                     VRFEYDYKPPAGTEEEKLLNVLLQPRDWLKINSVQS"
     gene            13736..14713
                     /gene="hemB"
                     /locus_tag="VGA21_06575"
     CDS             13736..14713
                     /gene="hemB"
                     /locus_tag="VGA21_06575"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012929849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEX9650205.1"
                     /translation="MTRRNRILRKSGSIRSIVQETSISANDFIVPVFVTEGKGIKEEI
                     SSMPDYFRYSLDQAINELKTCYAMGIKSALLFVKVPNNLKDNTGNEALNEEGLMQRSI
                     RKIKETLPDMVLMTDVALDPYSSFGHDGIVEGDEIINDKSVELLARMSVSHAKSGADF
                     VAPSDMMDGRVKAIREALEREGLHNTGIMSYAAKYASCFYGPFRDALESSPGFGDKKT
                     YQMNPANHIEAIKEVLADVDEGADIVMVKPAHTYLDIIREVKNTVNIPVSAYHVSGEY
                     AMIKAAARAGFINEEQAIIEVLTSIKRAGADIMATYFAKQAVELIQKNS"
     gene            14713..15999
                     /gene="hemL"
                     /locus_tag="VGA21_06580"
     CDS             14713..15999
                     /gene="hemL"
                     /locus_tag="VGA21_06580"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014799797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HEX9650206.1"
                     /translation="MHTLTQSKSKILFDRAQLHIPGGVNSPVRAFKSVGGTPIFISKA
                     KGAYLFDEDGNRYIDFINSWGPMILGHAYEPVIDAIRETAGRSTSFGAPTELEIKMAE
                     LIKSMVPGVDLVRMVNSGTEACMSAVRLARGYTGRNKFIKFEGCYHGHADPFLISAGS
                     GVATLDIQSVPGVPAEVAQHTLTAPYNNLSTVEELIEKNPDEIAAIIIEPIAGNMGCI
                     LPQPGFLEGLRNVCDEHGIILIFDEVMTGFRLSRGGAQELFKINADLVTYGKIIGGGL
                     PVGAFGGKKEIMEHVAPLGRVYQAGTLSGNPIAMISGFTLLNALNGNPEIYSKLEKIC
                     KYLADGIVDEFNKKSIPNRINRLGSMMSVHFCSHDVNNFEDAKKCDIPLFNRFFHHML
                     KNGVYLPPSAFETWFISAALTEEDIEATLLAVRKFD"
     gene            16014..17048
                     /gene="hemH"
                     /locus_tag="VGA21_06585"
     CDS             16014..17048
                     /gene="hemH"
                     /locus_tag="VGA21_06585"
                     /inference="COORDINATES: protein motif:HMM:TIGR00109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEX9650207.1"
                     /translation="MATKAILLANLGSPDSFSNKDVRKYLNDFLMDERVIDISFWKRI
                     LLVKGVIVPFRTSKSAAKYKTIWTENGSPLIYISGLVKEKLSKLTRLPVELCMRYGNP
                     TPSFALSKLHNDNPNLKEVVLVPLYPHYAMSSFETAIEHVKHAVAIGNFSFTLKVVPP
                     FFDNPSYIDTLANSIQPFLEKEFDHILFSYHGIPERHILKTDFTKSHCLKINDCCCQS
                     SSVHEVCYRHQVITTTNLAAKKLALPEDKFSYSFQSRLGSDPWLQPYTSEQLKKFPGQ
                     GIRKLVIVSPAFVSDCLETLEEIRVEGMTDFLKSGGEEFTAIPCLNDRDDWIKTLAEL
                     AKSKITELKG"
     gene            17059..17583
                     /locus_tag="VGA21_06590"
     CDS             17059..17583
                     /locus_tag="VGA21_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015333863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HEX9650208.1"
                     /translation="MQLYIKALHIIFIVTWFSGLFYVVRLFIYNQEAQEKPEEEKNVL
                     QKQFGIMIKRLWIGITWPSCILSLIFGIWLLFFYDTIPIWLWIKLGFVTGLLLYHISL
                     HFLYMQQSKKIFRYSSLQLRVWNELATIFLVAIIMLAVVRTGLGIILGISGLLALSVL
                     LLSGIKIYKYYRSE"
     gene            complement(17617..18039)
                     /locus_tag="VGA21_06595"
     CDS             complement(17617..18039)
                     /locus_tag="VGA21_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015267624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3995 domain-containing protein"
                     /protein_id="HEX9650209.1"
                     /translation="MTILSILLFIIFIILGLLHFSWAFGMHGGFADALPTKEDGKRVL
                     NPGKIDSAMVGIGLTAFGFFYLIKSELVANILPEWIIKYGSWIIPSIFILRSIGEFRY
                     VGFFKKIKKTRFATLDTKFYSPLCLGLGIIGLIIEIMK"
     gene            18180..18398
                     /locus_tag="VGA21_06600"
                     /pseudo
     CDS             18180..18398
                     /locus_tag="VGA21_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020603428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         5700 a         3471 c         3868 g         5502 t
ORIGIN      
        1 aagtagatgt gtttaacatc aatattatta atactgccaa cgacagcagt aaaaagccat
       61 atcccaattt tttcatgaca caatattttt ttgaaaagta gaaaaaatcc tcgttccaat
      121 attcagattt aatgaaattt atttaagcgg aaaaaaacta tcctaacaat ttgtcttata
      181 atgcatatct taaccctaac atccgggtga tatgatccaa ttgataagtg gtgaaagtgc
      241 ttttgcctat ggtgcaataa attcgggcgt ccggctaatt acaggatatc ccggttcacc
      301 gtcttccggt gctttcgaac ttctcaggaa tttacaaatc aaatttggtt accatatgga
      361 gtggtctctg aatgaaaaag tagcatgcga agttgccatt ggaggttcta ttggtggctt
      421 aaggtcattg atttgtacaa agagtgtagg gttgaatgtg atgattgacc cattgatggc
      481 tctgaatctc accccgctta acgcgggttt ggtgatcttg cttggcgatg atcctcaggg
      541 atatggttca caaaatgatc aggattccag ggccatagtt cattttttgg aaatgcccct
      601 gtgggaacct gcagcacctg cagatggata tttcatgatg attaacgcat ttcaggtttc
      661 agaagaattt ggagtccctg taatcattag ggaaacccgt agtttttctc aggtcaccgg
      721 tgaaattgat tttgaattaa aatatgatcc tgaaaagaag gagcttatcg aggaaccctt
      781 tcgctttgtc ccggttccgg aaaatgcagt tgcaaaacat ctcgccttgc agaaaagttt
      841 aagtaaagtt actatatggc atgaaaattc cgttttcaac gagacaaccg gcaacggtaa
      901 aaaaggaatt ttagcttcag gatttgtcac acataagttg ctggatattc tggagggggc
      961 agaaaaacat ccattcagaa tttttaagtt gggaacattg tttccactgc cttatagaaa
     1021 aattgaagaa ttcctgaatg actgtgatga aatattaatt ttcgaagaat cttcagactt
     1081 cattgaacga gcaatcaagg ttatagcctg ggagacccat tgcgcaacta cgatattggg
     1141 aaaaagtgaa ttagggttaa gccccgccgg ggagctcttc aaatggcaaa tttcaaatgc
     1201 attaaacaca ttgttcccgg atttgaaaat caaagtgtac ctggaaagcg cgcaggcgga
     1261 agaatatcct gagatgaaaa ataattgtag tgacagcaga tatgaagagg taatagagct
     1321 ggtggaaagg gcagcccaac taggtgggtt gacaccgctt tttgttgggg atccgggctg
     1381 tatatttatg gcaggagaaa aactaatcgc gaaatacgcc attggatccg cggtcggtct
     1441 ggcctccggt ttgcaatatg caaatcctga caaaccggtt gtcgcatttg taggagactc
     1501 aggatttttt cacaccacca tgccagctat tgctaacgct gccttcacgt taagaaattt
     1561 tcttatcatc gttctggata atggaggagc tttaaccacc ggattgcagc caactcctgc
     1621 gggtggaaaa aatattttga atcaggaatc aatgccgcaa agttttcaga aaattgcaga
     1681 atcactgggg attcagcagg tttctaaaat ttccctgaat gatgaacctg ataagattat
     1741 aaatgcattc ttagcagggc ttaccggtaa aaaactgaat ctgataatcg tccaaattcc
     1801 ttctactact gccggatgac ataactatta taaaatgttc gcgcaacaat gccttctttt
     1861 ctgaaatcat taattcctat cgggagaaac aaatgtatat ttgcttaagt cattgaataa
     1921 agtgataaaa acattaaaca gacggcagca caaattatcc gatccagcat ttcaggtatc
     1981 tgtactttct gatcctcagt caacaggtgt tgtcgaattt aatgagaagc ttgctggtaa
     2041 gggtttattg ccattaaaag catacctccc ggaaattttg cagattaacc tgggtaaaat
     2101 gtgcaatcaa acctgcagac attgccatgt tgatgcgggg cctgatagaa cggaaattat
     2161 gacaaggcaa actatgcaag aaatccttgc aaagattcct ggaagcccaa tctccacttt
     2221 agatttgacg ggcggagccc cggaattaaa tcctgaattt cgatggtttg ttgacgagtt
     2281 aatgaagttc aaaaagcata taatggtacg cagtaatctc actattatag tttcgaacaa
     2341 gaagtaccat gatttgccgg aattttttcg gcaccacgat atagaaataa ttgcttcact
     2401 gccttacttt tccgcaaaca gaacaaatgc tcagcggggc gaaggagttt ttgaaaagtc
     2461 tgttcaggca ttgaagatgc ttaataaagc aggttatgga agtgagaatt caaatctaaa
     2521 acttaatttg gtttataatc ctacgggagc atttttaccc ggaaatcagc aaggacttga
     2581 gcgggaattt aagaaccggt tacaggccga acacggtata gtgttcaata atctttttac
     2641 catcacgaat cttccaatta gcaggttcct ggattatctc atttctacag gaaatctgga
     2701 aagttatatg cacaaactgg tgaatgcttt taatccggcc gccagtgaaa aattaatgtg
     2761 ccgcaataca ttgtcaatag gatgggacgg cagtcttttt gattgtgatt ttaatcagat
     2821 gcttgaccta agtttgaatc atgggatgaa tcatatcaag gatttgaact ggagaagttt
     2881 ggaaggcaga gaaattatta ccaacagaca ttgctttgga tgcacagccg gtgccggctc
     2941 aagttgcggt ggggcgctca cgtaatatat ggttaacccg gtagtattca tcccatgtta
     3001 ttaatttagg tatttgaata tgttgatgga cattatcgag ttttccacgg tttttattta
     3061 ttgcttttgc ctgcttatga tttttctctt tagcttaggg caactccatc taacctggca
     3121 ttatatcagg aataagaaac gtacaaccct ttctcccgga gaattaagta cttatcctaa
     3181 agttaccgtg cagctcccgc tatataatga aaagaatgtt gttgaaagac tgataaacaa
     3241 aatagtacaa ctacgttatc cgaaagaatt gcttcaaatt caggttcttg atgactcgac
     3301 tgatgaaaca agtttaattg tttcgaaact tgtttctaaa tacaaaattg aaggttattt
     3361 gattgatcat ctggtgcgga atgaacgagt tggattcaag gccggcgcat tgcaaaatgg
     3421 tctgcaaaaa tctaccggag aatttatcgc tatctttgac tcggatttcc ttccgccagt
     3481 aaatttcctg ctgaaatcat tgcctcattt tcaggataaa tcggttggga tcgttcaaag
     3541 caggtggggg catttaaatg aagactattc cttgctcaca caaattcagg cgtttggcct
     3601 taatgcacat ttttcgattg aacaatcagg tagaaaagcg gcagggagtt ttataaactt
     3661 taatggaacc ggaggtgtct ggagaaaaac gtgcattgaa gatgccgggg gctggtcagc
     3721 tgataccctc actgaagatc tggatttatc ttatcgtgcc cagcttaaga actggaaatg
     3781 cgaatatttg gaggatttaa tttctccggc agagttacca attcttattt ccgcaataaa
     3841 gactcagcaa ttcaggtgga acaaaggggc cgctgaaacc ggaagaaaat tgatcaaaca
     3901 ggttattggt tcatcaaaaa gaaaaattaa taagcttcat tcaataatgc acctctttaa
     3961 tagttcggtg tttttattct tattcattgc cggagtactt tccattcctg ttctgttttt
     4021 taagcaggga aatcgaaatt atgaaattct gtttgatgcc ggagcatttt ttttgatcgg
     4081 attctttagc attacttttt tttaccgcgt agcttacaag aacattaagc cctcgtctgg
     4141 taaaagtttt gcattcttat ttcctgtttt cctggttgtt ataatggggc tttcccttca
     4201 taattctgtg gctgtcctgc aaggcttatt tggattaaaa agccccttta agcgtacacc
     4261 aaaatttaat gttttcagga aaggcgatga gtggaaatcc aaaagctatt ctgaaatcaa
     4321 agcaaataaa ataagttgga cggaaggatt aatttcattg tacttcctgg cggggttagt
     4381 ggcgggtatt tatttgggtg acttcaaatt gatgctgttt cattgcttgt tattttcagg
     4441 tttcacttct gtattctatt tatctattaa acatcagttt gccggttaaa cttagctggt
     4501 accttgaagg aaaaggtcca tatcacccta aatttattat agctttgtct gattaaaaat
     4561 aaatttaatt tttgagagag ttttgcataa agtttggtat agcatgccta tgaggtttcg
     4621 tgtgacgatt tttggggtct tattaatttc tgcacttttg ggctgccaga aagagacaat
     4681 aatgcccgga ataaccgggg gaagtttgaa ggctgaagtg aatggaaaca cctttgaggc
     4741 gacagggatt ctggccttca ggactcagcc tacatcaacc accggagaag gattttatgt
     4801 ttccggcaac acaaccattg gttttcctca aattacttta accctttata gcagcttaca
     4861 gggtaagtat caacttggcg tttccggttc aggaaatacc caggcatcat atgcattatc
     4921 cgtcgacaat caggaattgt caatttcagg taacattaac attacaacaa taaattattc
     4981 ggaaggtgga actgttaagg gcacttttga atttgcaacc gccactaaca gtattacgaa
     5041 tggctctttc gaaattcgat tttcgaacta aagaaattca gattttcttg caaagtattt
     5101 tgaagaaagc gtttgaggca atttggtcac aaactatgat tgaagagaaa aaattatgag
     5161 tgaaaaggaa atagtaaagc aacaatatcc tgaaagtgaa ttgactggaa aaattattgg
     5221 ttgtgcaatt gaggttcata aagtgctggg taatggattt caggaagtta tctatcagcg
     5281 agccttacag attgaaatgc gaaatcagca tttggaattt agtagagagc atgaaatgga
     5341 aatattttat aaaggagagc aaataggcac cagaagggtt gacttttttg tagaagggaa
     5401 aataatggta gaactgaaag ccgttattca attggaagac gtacacttag cgcaagcaat
     5461 taattattta gaagcgtata acatgcaaat tggtttgctc ataaatttca gttagtcaaa
     5521 gtttgcaatt taaaagagta atgaaaccaa agcgaaaatc acagtcatca gaaaatcaaa
     5581 cgaatcatag ttttgctcat aaatttcagt tagtcaaagt ttgcaattta aaagaagtaa
     5641 tgaaaccaaa gcgaaaatca cagtcatcag aaaatcaaac gaatcatagt tttgctcata
     5701 aattccggta gtcaaagttt gcaatttaaa agagtaatga aaccaaagcg atggaagatg
     5761 tgccatgaat cgggtttttc acgcactgaa tccgggaaaa aattccctaa aatcagactt
     5821 ctgatgaata tcatgtttta tatgccggat ttttacctta tttaataaaa taagattatg
     5881 gaacttgcaa tgcatcccaa cgaattgctg attttgttta atcgcgattc cgctgtcgcc
     5941 atgaagaccc tggcatttgc caaaacagtt tcaaaccata tccgggacat ggaatttaga
     6001 agtctaaaat ttccaaatac tatttggaaa gaattattga atatgttgaa tcttgaagca
     6061 aaagatattt tggataaatc ccacccggac tatcaaaaga aaattatggg tcatgatttc
     6121 tccactgaag ggtggttaaa tgtgttatca cacaattctt atttaataaa agctccaatt
     6181 gccgttaaga acgggaaagc agttttatgc aatcggccaa aagatgtact gaagctgata
     6241 aatgaagaag ataaaattcc ggtttcttag aatttatcac ttttttttcg gaaggtcaca
     6301 taagttgcaa aagaataaat ggaacccctt tgaatcaaaa tttaatcgcg aaacctgcag
     6361 ttatatacct tggcaaacca acccggattc cctccggcct tctcgtggca atgtatcgct
     6421 catcggtaac attcttgatt gagatattta ttgtcgcgtt cattgaggat atgatatgat
     6481 acagcgtggc atccagcaca ttccggcttc caattttccc aatgcgccca ttaaatgatg
     6541 cgttaacagt atttaattca tcagtaaact gattgcctat ataattatca gttagccgca
     6601 atcctgttcc aaaaggagcc tcaatgctga acccgcatga caaagtgaat tcaggcgcat
     6661 aaggaagtct gtttcctctt acattaacct gatcatttcc tgatgatacc agacggtcag
     6721 aattgtaaat cgccttaata gttgtagcac tcaagtcaaa ttggattttc caattcgatg
     6781 tcagaaattc acgactatct aaagtaaagg aagtttcaaa acctttgtgt aaggttcgtc
     6841 ctgcatttgt tactccgaat cccggaccac cgattgactg tgaagatgga ataatttggt
     6901 tttcaaagtc catgtaaaac catgtgactt ccaaaaacat accttttatg ggttgtgacc
     6961 gcaaaccaac ttccagattc cagctttttt cagcttcaag ttccagcacc ggattcaccg
     7021 cgctaaagtc aatagcatct ttaatccgtg gcggagcgta gccgcgatga attcccgtga
     7081 aaaaatttaa ttttgaagcc ggtctgtaat taactccaaa gccgggaatg acttcacgaa
     7141 tttggttatc agccactcta ttggtatcta tatttgcttc tcttaaaatt tctctctcgt
     7201 aagaatagaa ttccactctt acacccgggg taatatccag tttctgcgaa atgctaaact
     7261 tattcaaagc aaataaggag agtgcgtttc cggtacggat ttcatcgctg attaaatcgc
     7321 ctgattttgc ctgatcattg gtaccgttga ttctctgctc ataagctctt tcatacagaa
     7381 tgcggtaacc tgcaattaat tcattgttga aatttccgaa ggatttatta tgaagaattt
     7441 tctgttccca tccaatgact tcaaattgcc ggtttcgatt tccggtacgg tctcgtaaat
     7501 aaattgctcc accatcggtg ctttcatctc ccaaaactac tccggtccaa tcggatggtg
     7561 gtggatttgg attcgttgca gtatacgaat tgtaggaata gccttgcctc cgccaatttc
     7621 gggtcgtcgt atacccgtaa agcattgatt gaagatttat tccttttctg aggttaatat
     7681 tattagaaaa gctcattaaa taacggcgta catcaagctc atcatttggc atcagtacgg
     7741 aaaagtcata accgccggtt tcaaacattg attgagtgag accaacataa gttgaatttg
     7801 aaacctcatc atatgctgac aactttattc cgaatgttgc tcgttcagaa agaaggaatt
     7861 tcacctttgc tgtgaagtcg ttaacagtga aatttaaggc gccaaaatca tcagcccgct
     7921 tatggagata atctatttga acccctgttt tattaaatgt atttccgtaa ctccccaaag
     7981 cggtaagaaa ggtgcctttg ccaccttgca gcttcaaacg aatctcctct ttttccggag
     8041 gagccgcggt tatataatta attacacccc caacggtttg tggcccatat aaaatttggc
     8101 cgcttccttt tagtacttct actccattca ttctatcaat agcgggcgag taatacattt
     8161 caggttcacc atatgggttc agagcaacag gcacaccatc ttccattatc agaacgcttc
     8221 ggcttctgtc aggatctaat ccgcgaatac caatattcac ccggagtccg gcaccttctt
     8281 catccactac atttaatccg ggaatcctgc ggaagacttc gtttccgctt atgggtttta
     8341 acttatctaa ttcattatta tcaatataag aaacggatcc aggtattttg ctaaatagca
     8401 gattgtgctt atttatgacg aaaacctgtg gcaatacatg tggattttcc tttaacgtaa
     8461 tcaaaaaatc ttcttttacc tgagtattaa ttttttcttc gaatggaaga aaactgacat
     8521 gcgttataag aattatgaat tgctcttccg gaacattatg aatttcgaaa ttaccattta
     8581 cgtctgtaat cgatgtcctt tgtgtttctt ttatctgaac aagtgcatgt tcaacaggat
     8641 aacctgattc atccgtaatc cggccggaaa agataattcc ttgctgcgat tgcgcaaatt
     8701 ccggcaacaa aaatccaaat aacataattg aatatttcag cataaaataa gtagtatttt
     8761 tggtttattt agaatgatta taaataagaa gccaatatta tgtcttgttt agaattgatc
     8821 caaacaagga ttgagaattt tgaaaatcaa atctattttg ggtattaatt atccgtttga
     8881 gggcattttt ccttttgctc tacaggtata tttggctcat ttcttaagtg aaggacgcat
     8941 tcatgggcat aaattcaaca tttttcaatc gtttggaaaa ctttgtagtt attggttcaa
     9001 atttcacgaa agccgatata aatacccgaa gtcgcttcgc catcacagga cctcattccg
     9061 agaaagttta ccgaaaagcg gtggatatgg gttttggtga ctttctactg ctttcaactt
     9121 gcaaccggac ggaattctat agttgcggtt ctgttacaac attaagaagt ttagtgaaac
     9181 aacaattatt gctgaccgat gaggacctgg atacgtattt ctatacgtat tccggaactg
     9241 atgcaatcca gcatttcttt cgggtattgt caggtttaga ttcacagatt attggtgagt
     9301 atgaaatagt gaaccaggta aagtcagcac tgaaagcatc gcgttattat gggcttacag
     9361 gtacaatctc tgatcgcatt tccaattttg ccctccaggc atcaaaggaa attaagtcag
     9421 aaactaatct gagcaaaggt aagtattcag taagttatgc cgccgccgaa ctgatgctgg
     9481 aaaagcaaaa agcgcaatta gtggaaaaag ttctgatcgt gggtaccggt gatttcggcc
     9541 gcacagttgc cagaaacttc cgacagtatt ttccggatat taacctcagc ctcacaaacc
     9601 gtacacaaaa aaaagcccaa caactagcgg tacaacttaa tgcaaatatt ctgccctttg
     9661 aaactttcaa aaaacatctc cacacatttg atgcggtaat tactacgata ggtactgaga
     9721 agtacatgat taatccaggc gacattccac taaacaaacc tcagctttat ctggatttaa
     9781 gtgttcccca ggcaattaat cccagagtag atttaataac ggatgtccaa ctttattcag
     9841 tggatgaaat ttcagcattc cataacgatc tcctggcaga acgtatgtca gaaataccag
     9901 aagcggaaga aattattgca caacatattg accggttgct tgcatggaat aatgttcata
     9961 aacaccggaa gataattttg ggttacaagg aaaaaattca aaaccttgtc agcaatcaaa
    10021 gggatgccca tgtaaatggt aactcccgcc tcatcaacca caagatagat aaatcctttt
    10081 cagaattaat tcaaaaaatc aaaacgcatg gatacgcggg ttgcataatg attgaagcga
    10141 tggacgcgtt tgttcctgca gaaaaatgac acaatcgaat attatccgta tcggtacacg
    10201 ggaaagcgcc ctggctatgt gtcaggcaag ggaagtgcag tcgaaactgc acgaattgag
    10261 tatcccttca gagataatac aagtgatttc ggatggtgat gtggacctgg aaactccgct
    10321 ttatgaaatg gggatccagg gaatttttac cagaaccctt gataatgcat tgttacaaaa
    10381 gaaggttgac atcgctgttc attcagctaa agacattcca acacgtttgg cttccgggct
    10441 ttgtctggct gcagttctgg aacgtggttc cgaatgggat accctcgtcc aacttcatag
    10501 ctcgcctgcg attgatctca caaatacctg tacaatcgca accagtagtc tgcgaaggaa
    10561 aacacaatgg ctttatcgat acccctgcca caagattgag agtcttcgag gtaacatcca
    10621 gacacgtctt gataaactta ataacggcag ttggcagggg gcaatctttg cccagtcggc
    10681 acttgataga ttgaaagtaa attctaatcc tgttacaata ttatactgga tgctgccggc
    10741 accggcacaa ggcgccatcg ctatggtttg ccggattgat gatggacttt caagagaagc
    10801 atgtaatcaa attaaccaca gcgaatctga gatttgtata aaagttgaac gcgaattttt
    10861 ggccgcatta cacggaggat gtgcggttcc aattgctgca tttgctaaga tccataatgg
    10921 cagcatgtca ttcaagggta acattttgag cctaaacggc aaacaaaagt gtgaagtaga
    10981 aatgcaattc gaaccagatc aaacgaaaga cgccggacaa gttgcagccg ctgaaattat
    11041 taagcgcggt ggtgaatcta tattgaaaac ttttcggaag gtatgacaag aaagttacat
    11101 atcgtatcaa cacgctgcct tgattccttt aattttcaaa agctaaatga aaaggaattc
    11161 actttttcca gtgttgattt tattagaaaa aaggtagata ttcctgtaaa cataagtaaa
    11221 ctttcctttc ataaagatat agtaatcacc agcaaaatgg gggtgatatc atttttgcaa
    11281 atagccagga tgaaaggcat tgatttacag aaatgttcca tttattgttt aagccatgga
    11341 actcgaaaat atgcgaagaa ttcaggtttg aaaattgtta aaactgcctc taatgcttct
    11401 aatttagcag aggagatact gaatgaccct gacgtaaagg ctgttacata tatctgcagc
    11461 aataagagga gaaatgaatt gtcggaaaga ttaattaaag cggaagtaat tgtgcaggaa
    11521 gttatcgctt accgtactga cctcactcct gcaaaaatcg agcaacctta tgatgccatt
    11581 ctttttttca gcccaagcgc ggtggaaagt tttcttaagc ttaatccgct taaacccgcg
    11641 gcgtgttttt gcctgggtga aacaactgct gattacgcac tgaagcatgg atatgttgat
    11701 attcatattc ctccaattcc cacagaggaa tccctgcttg aaactgtaat aaatcacttt
    11761 tcaatttacc ctgctcatgc tatgtaacga tctgttatta cgagcactac ggcaggaaaa
    11821 tctgatacgt ccacccgtat ggatgatgag gcaggcggga cgctatcttc cgcagtatat
    11881 tagattaaga aaaaagtacg atttcttcac tcgtgttcaa acgccggaac ttgcatgtga
    11941 aattactctt caaccggtgg atgtaattgg agtagatgct gctattatct tttccgacat
    12001 tcttgttatt ccacaggcaa tgggtatgaa agtgttgatg gaagaaggaa agggacctca
    12061 tttgcccaac cctatccaga ggttgcacga catcgaaatg ctcgatattg aatcaataag
    12121 taagcaatta aattatgtct ttgaggcttt gaaactaacg aagcaagagt tgaataatcg
    12181 agttccactt ataggttttg ccggcgctcc ctttactctg ctttgttata tggttgaagg
    12241 aaaaggaagc aaaggatttg aaaaagctaa agccttttgc tttacacagc cggagttggc
    12301 tgaacgtctg ctgaataaga tcaccctggc aaccatagat tatctgaatg gtcaggtaaa
    12361 agcaggcgcg gatgttatac agatttttga cagctggagc ggattactga gtccagatga
    12421 ctttgaccga ttttcggctc cttaccttat acgtattgcc gaagccttga cgcaacaggt
    12481 gcccataatt cttttcccac ggggcagcaa ttatgcattg aaatctttat cgcaaaacta
    12541 cgccgtgagc ggtattggaa ttgactggac aataagctct gcacaggcaa gagaactaac
    12601 tcaatacaac gtggctttgc agggtaactt cgatcccatt aaactattaa tgccgccggc
    12661 ggaaataaaa aagcaggtat ggaagatgat tgatgaattc ggcaccaaag gatatatagc
    12721 taacctcggt cacggaattt taccatatat acctgttgac catgctaaag catttgttga
    12781 tgcggtaaaa gaataccggt atgagccaat taactaaaga acactttaca agccatattc
    12841 gaactttgca aaacacaatt tgtgaagaaa ttgaaaaagt tgatggtaag ggaactttca
    12901 attctgactt atgggagcgt gcggaaggag gtggcgggga aacccgggtc attagcaatg
    12961 gaaatgtgtt tgaaaaaggc ggtgtcaata tatctgttgt tcatggaaaa ttatctcagc
    13021 ctgccgcaaa gcaactcctg attaatggag attcgttttt tgcctgtgga ttgtctctcg
    13081 ttattcaccc atttaatcca tttgtgccta cagtgcacgc caactggcgg tactttgagg
    13141 tctatgatga taattcttta ctgctggatg cgtggttcgg gggaggcact gatttaacac
    13201 cttactattt aattgaaagt gacgtcgttc attttcattc cgtgcagaaa caaaattgcg
    13261 ataaatttga tacttcattg tacccggttt ttaaggaaca atgtgataat tattttagca
    13321 atcaccaccg tggaggtgaa cgccggggag ttggcggaat attctttgat caccgcaggg
    13381 aaactgcagg aaaatccata gagttttgga ggaaatttac agaagcaaat agtttagtat
    13441 ttaccgatgc ttaccttcca attgtgttaa agcgaagaaa tacccatttc acctctgaac
    13501 acaagttttg gcaggaaatc cggagaggac gatatgttga atttaatttg cttcatgacc
    13561 ggggtacttt attcggaatc agaaccaacg gcaggagcga aagtatcctg atgagccttc
    13621 ctccagttgt ccgttttgaa tatgattaca aaccgccggc cggcactgaa gaagaaaagt
    13681 tattaaatgt acttttgcag ccacgagact ggctgaaaat taattcagta caatcatgac
    13741 aagaagaaat cgaattttgc gaaaatccgg ttccatacga agcattgtgc aggaaacttc
    13801 gataagtgcc aatgatttca ttgttccggt tttcgttact gaaggaaaag gtataaaaga
    13861 agaaatatct tcaatgcctg attatttcag gtactcattg gatcaggcga ttaatgaatt
    13921 gaagacatgc tacgcgatgg gaatcaaaag cgcattgtta ttcgtaaaag tacccaacaa
    13981 tttgaaagat aataccggta atgaagcatt aaatgaagaa ggcctgatgc agcgcagcat
    14041 tagaaaaata aaagaaacat tacctgatat ggtattgatg actgatgtag cgcttgatcc
    14101 ctattcttct ttcggtcatg atggtattgt agaaggtgat gaaatcataa atgataagtc
    14161 agtggagctt cttgcccgta tgagtgtaag ccatgccaaa tcaggcgccg atttcgttgc
    14221 acccagcgat atgatggatg gaagagtaaa agccatcaga gaagcgttgg aaagagaagg
    14281 actgcataat acgggaatca tgagttatgc cgccaaatat gcctcgtgtt tttacggtcc
    14341 tttccgggat gcacttgaaa gttcaccggg gtttggagac aagaaaactt atcaaatgaa
    14401 tcctgctaac cacattgagg caattaaaga agttttggct gatgtagacg aaggtgcgga
    14461 tatcgtgatg gttaaacccg ctcacactta tcttgacatt atcagagagg ttaagaatac
    14521 agtaaatata cccgtaagcg catatcatgt cagcggagaa tacgccatga taaaggcggc
    14581 ggccagagcc ggttttatca acgaagaaca ggcaattatt gaagtgttga cttcaataaa
    14641 acgggcagga gctgatatta tggccaccta ttttgcaaaa caagctgttg aactcattca
    14701 aaagaatagt taatgcatac actcacacaa agcaaaagca aaatcttgtt tgatcgcgca
    14761 caactgcaca ttcccggagg agtaaattct cccgttcgag cttttaaaag tgttggtggc
    14821 actccaattt tcatcagcaa agcgaaaggt gcatatctgt ttgatgaaga tggtaatcga
    14881 tatatcgact ttatcaattc ctgggggcca atgatcctgg gtcatgctta tgaacctgtg
    14941 attgatgcaa tccgtgaaac cgccggtaga tcaacttcat tcggcgcacc gacagagctg
    15001 gaaattaaaa tggcggaatt aataaagtcg atggtgcctg gcgtagacct ggtaaggatg
    15061 gtgaatagcg gaacagaagc ttgcatgagc gccgttcgcc tggcacgagg atacactggc
    15121 cgcaataaat tcattaaatt tgagggttgc tatcatggtc acgcggatcc atttctgata
    15181 agtgcaggaa gtggcgttgc tacattggat attcaatctg ttcccggtgt tcctgcagag
    15241 gtagcacaac atacgctcac ggcaccgtat aataatcttt ccacggttga agaattaatt
    15301 gaaaagaatc cggatgaaat cgcggcaata attattgaac cgattgccgg caacatgggt
    15361 tgcattttgc cgcagccggg cttcctggaa ggcttgcgca atgtgtgtga tgagcatggg
    15421 attattttaa tttttgatga agtgatgaca ggttttaggc tttcaagggg aggtgctcag
    15481 gaattattca aaataaatgc tgatttggtg acgtacggaa aaataattgg aggaggattg
    15541 ccggttggag ctttcggcgg aaagaaggaa ataatggaac atgttgctcc gcttggcagg
    15601 gtttatcagg ctggaacctt gagtggtaat ccgattgcta tgatatcagg atttaccttg
    15661 ctgaatgcat tgaatggcaa tccggaaatt tattcaaagt tggagaaaat ttgtaaatat
    15721 cttgcggatg gtatcgttga tgaattcaac aaaaaatcca tacctaaccg gataaacaga
    15781 ttaggcagca tgatgagcgt tcatttttgt tctcacgatg tcaataattt tgaggatgcc
    15841 aaaaagtgtg atattcctct attcaaccgg tttttccatc acatgctaaa aaacggagtt
    15901 tatctgcctc catctgcctt cgaaacctgg ttcattagtg ccgccctgac cgaagaagat
    15961 atcgaagcaa ctctacttgc ggttagaaaa tttgattaaa tcaagctttg aaaatggcaa
    16021 caaaggcaat tttattggct aacctcggtt ctccggattc tttcagcaac aaggatgtaa
    16081 gaaaatacct gaatgatttc ttgatggatg aacgggtgat tgatatttct ttttggaaga
    16141 gaatactttt ggttaaagga gtaatcgtac catttcgtac ttcaaagagc gccgcaaaat
    16201 ataagaccat ctggactgaa aatggctctc ccctcattta tatttccgga cttgtaaaag
    16261 aaaaattatc aaaattaacc cggcttccgg ttgaattgtg catgcgatat ggtaatccta
    16321 ctccgtcttt tgctctaagc aagttgcata atgataatcc gaatctgaaa gaggtagtac
    16381 ttgttcccct ttatcctcat tatgcgatga gcagctttga gactgccata gaacatgtta
    16441 agcatgctgt tgccattgga aacttttcat ttactctcaa ggtagttccg ccattctttg
    16501 ataatccatc ttatattgat acattggcaa actccattca acccttcctg gaaaaagagt
    16561 ttgaccatat actattcagt tatcatggaa ttcccgaaag acatattctg aaaaccgatt
    16621 ttaccaaatc acattgcctg aaaattaatg attgctgttg tcagtcatcc agcgtgcatg
    16681 aagtttgtta ccgacatcag gtaattacaa ccactaactt agcggcaaaa aagttagctt
    16741 tgccggaaga taaattcagt tattcctttc aatcacgtct tggttcagat ccgtggttac
    16801 aaccttatac cagtgagcag ttaaaaaaat tcccgggtca gggcatcaga aaacttgtga
    16861 ttgttagtcc tgcttttgtc agcgattgtc ttgaaacact tgaagagatt cgggttgaag
    16921 gtatgacgga tttcctgaaa agcggcggag aagaatttac cgcgatacct tgtctgaacg
    16981 atagagatga ctggatcaag acattggcag aattggcaaa atccaagatt accgaattaa
    17041 aaggttagta ccatctttat gcaattgtac attaaagccc tgcacatcat ttttattgtt
    17101 acctggtttt cagggctttt ttatgtagtc cggttattta tatataatca ggaagcccag
    17161 gaaaagcccg aagaagaaaa aaatgtgttg caaaaacaat ttggtattat gattaagcga
    17221 ttgtggattg gaattacctg gccctcctgt atactttccc tcattttcgg tatttggctc
    17281 cttttctttt acgacaccat tcccatttgg ttatggatta agctgggatt tgttaccggt
    17341 ctgcttctgt atcatatttc actccatttt ctatatatgc agcaaagcaa aaagattttt
    17401 cgatattcaa gcctgcaact ccgcgtctgg aatgaactgg caacgatttt tctggtagcc
    17461 ataattatgc tggcagttgt tcgcaccggt ttgggaatca tattgggaat ttccggtctt
    17521 ttggctctta gtgttcttct tttgagcggg ataaagattt ataaatatta tagaagcgaa
    17581 tagcaaattg aattcagatt tagggactaa tttcttttat ttcatgattt cgattatcaa
    17641 gcctatgatt cctaaaccca agcaaagtgg cgaatagaat tttgtatcca gggttgcgaa
    17701 tcttgtcttt ttgatttttt tgaagaatcc aacgtatcta aattcgccaa ttgatcttaa
    17761 aatgaaaatt gaggggatta tccaacttcc gtatttaatt atccactctg gtaaaatatt
    17821 cgcgacgagt tcagatttta tcagatagaa aaatccaaat gctgttagcc ctatgcctac
    17881 catggcactg tcaatctttc ccggattcag tactcttttc ccatcctcct ttgttggaag
    17941 tgcatctgca aatcccccat gcatgccgaa agcccagcta aaatgaagta atccgagaat
    18001 aataaaaatg atgaagagca ggatggataa gatagtcatt tgtttccttt tggttgtgct
    18061 acgaaagtgt ctttcaattt ggtatgtgag tacttatctt tagctaccaa tgttttaggt
    18121 tagcgtcatc gtgacaaaag taaatataat gatataatag taatttccga atacgctatt
    18181 ttatcaatga taagaaatgg gtaaaaggta aatttaattg gcaggaaggc tttggtgcgt
    18241 tctcttattc aagaagccag attgatacgg taatcaaata tattttgcat caggaagaac
    18301 atcataaaaa gaaaaccttc agagaggaat atattgattt cctgaaaaaa tttgaaattg
    18361 aatacgatga aaaatatttg tttgaatgga tagaataaaa caggtcgctc ctacggagct
    18421 tacggttgtt gtattctgcg ataactacaa acagaatgct cctacagagc aatgattaga
    18481 aatgaataaa tgaagaagat agacaaactc attatcaatt ctcctttctt taaatcaaag
    18541 t
//
LOCUS       DASWHX010000098         4448 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_818779, whole genome shotgun sequence.
ACCESSION   DASWHX010000098 DASWHX010000000
VERSION     DASWHX010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 4448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4448
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_818779"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /locus_tag="VGA21_06605"
     CDS             <1..241
                     /locus_tag="VGA21_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011585457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9650210.1"
                     /translation="IMEEFGLRVNSIRVQLFISLLDKLFRMFQRLHKQSNFQGTGVGL
                     ALVKKIIDRHHGRIWADSAVNKGTTFYFTLPDGRV"
     gene            306..833
                     /locus_tag="VGA21_06610"
     CDS             306..833
                     /locus_tag="VGA21_06610"
                     /inference="COORDINATES: protein motif:HMM:NF027592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4920 domain-containing protein"
                     /protein_id="HEX9650211.1"
                     /translation="MKRTITVLSICLSFAACTAEKSSHKEHEDPAIETEAISQKPESK
                     TGIFGLEVSREDALDVASVYLTELAAEGVEVKVKGEILEVCQSMGCWVTLKLPDNKSV
                     RVSTDHKFTVPKDISGYTAIVQGMLNKKITSVEDLRHYAEDAGKDAAYINSITEPEEE
                     YSIDAVGILVLEQEG"
     gene            complement(830..1201)
                     /locus_tag="VGA21_06615"
     CDS             complement(830..1201)
                     /locus_tag="VGA21_06615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650212.1"
                     /translation="MKIFIKIILISLIGYGLQLFLPWWYVVVASFFINLIIYSKGWQS
                     FMAGFWGIFLLWFIAALVIDVKSHSILSDKVAQIFQLPNPMVLILATALIGGISGGFS
                     GLTASLLLNIFRKKRSDYYYS"
     gene            complement(1198..3621)
                     /locus_tag="VGA21_06620"
     CDS             complement(1198..3621)
                     /locus_tag="VGA21_06620"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HEX9650213.1"
                     /translation="MKVIKFIFSLLLTGVLVFALNTKFAQIPPLGKFLDPFHGFWQNS
                     YTGPENYADFELQGLKDEVTIIYDSLLIPHIFAKNNKDLYFAQGYVTAKERLWQMEIT
                     THASAGRISEIMGPAALDFDRLQRRKGMVFGAKNSMTLIAEHPESSEALESYAAGVNR
                     YIQSIKYKNLGVEYKLLGYEPEEWQPLKTALLFMSMANTLNMAEKDLQNTNFLKLYGF
                     ELLNLLYPDYEGVSDPVVDNPGGWDFNPIILDNIPLAIPEGLYESELLPEADPNVGSN
                     NWAVSGKKTQSGHPILCNDPHLELNLPSIWYIIQLNAPGINTMGASLPGAPFVVVGWN
                     DSIAWGETNAQRDLIDYYKITFKDESMDRYLLDDIWVPTQKVIEEIKIKGQESLYDTV
                     IYTNWGPVIYDRSFRPESERKNLAFRWISHDASDPALTFYKLNKAKNYHDYKDALDHY
                     SSPAQNFAFADVNGDIAIRVQGKFPVRRKDEGRFVLDGSKSFNGWKEFIPNEHNIQTL
                     NPERGFVSSANQYPADNTYPYYITASNFEAFRNRRINYILDSLNEITAEDMMRLQNDN
                     FNLEASDALPALLGYLNPFGLDTEEKIIYEILKSWNYYNNPDSEAASYYEIWWTTFEQ
                     MLWDELRNAKVSLRYPTDYTTIQLVKDSVQLELYDRQETPESETLAEVIESSFKETIT
                     RANKWKAGNGEKIPWAVFKGTTIKHLVQQLEAFHIPVYNGGNGGIVNATSGRNGPSWR
                     MVVSLDPSGVKAWGVYPGGQSGNPGSPFYSNLISYWESGKYYPMLFLNDANANNASNV
                     SKQTLTPAK"
     gene            3730..4257
                     /locus_tag="VGA21_06625"
     CDS             3730..4257
                     /locus_tag="VGA21_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009197070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HEX9650214.1"
                     /translation="MKEILNLYPGEIVKKAKKIKTLLFDVDGVLTNGKIIYMGDESEI
                     KEFHVRDGQIIKPLREAGILVGIITGRVSEAVLRRVLELEFDIIRQGIKNKLECYEKI
                     KSENGLIAEEVAFIGDDFPDVEIMENCLLGICPADAPDYIRLRADWVTKAKGGEGVVR
                     EVADLILSAQGKFPI"
BASE COUNT         1353 a          902 c          910 g         1283 t
ORIGIN      
        1 cataatggaa gaatttgggc tgagagtaaa ctcaataagg gtacaacttt ttatttctct
       61 tttggataaa ttattccgca tgtttcaaag acttcataaa caatcaaatt ttcaaggaac
      121 aggtgtcggc ctggcccttg tgaagaagat aattgacaga catcatggca ggatttgggc
      181 cgattcagcc gttaataaag gcacaacttt ttactttacc ttgccggatg gccgagtttg
      241 actttataag aataaaattt tgattaattt tgcacccata acatctttaa tttctaaaaa
      301 ataaaatgaa aagaacaatt acagttttga gtatctgcct gagttttgct gcctgcacag
      361 ctgaaaaatc ttcgcacaag gaacatgaag acccggcaat agaaaccgaa gcaatctccc
      421 aaaagcctga gtccaaaacc ggaatttttg gcctggaagt ttcccgggaa gatgccttgg
      481 atgtggcgtc tgtatatctg accgaacttg ctgcagaagg agtggaggtc aaagtgaaag
      541 gagaaatcct ggaagtttgc caatctatgg gttgctgggt aacgctaaaa ttaccggata
      601 acaaatcggt ccgcgtaagt accgatcata agttcacggt gcctaaagat atttccgggt
      661 atactgcaat tgttcaaggt atgttgaata agaaaattac ttcggtcgaa gatttacgtc
      721 attatgccga agacgccggg aaagatgcag cttacatcaa ctcgattact gagcctgaag
      781 aggaatattc tattgatgca gtgggcatac tggttttgga acaggaaggc tagctataat
      841 agtagtcact tcttttcttc cgaaaaatat tgaggagtaa agaagcagtc aatccactga
      901 atccgccgct gattccgcca atcagtgccg tagccaaaat caaaaccatt ggattcggta
      961 attgaaaaat ctgagccact ttatcagaaa ggatagagtg gctttttacg tctattacta
     1021 aagccgcaat aaaccataag aggaaaatac cccagaaccc ggccataaat gactgccatc
     1081 cctttgagta aataatcaaa ttaatgaaaa aggaagcaac cacaacatac caccagggca
     1141 gaaatagttg aagtccatac ccaatgagtg aaatcaatat tattttgatg aagattttca
     1201 tttggctgga gtcagggttt gcttacttac attacttgca ttatttgcat tagcatcatt
     1261 cagaaataac atcggataat atttgccgga ttcccagtat gaaatcagat tgctgtaaaa
     1321 gggactgcct gggttgcctg attggcctcc cggataaacg ccccaagctt ttactcccga
     1381 ggggtcaaga ctaaccacca tcctccagct ggggccgttc ctgccggatg ttgcatttac
     1441 aatccctccg tttccaccat tatacaccgg aatatgaaat gcctccaact gctgaaccag
     1501 gtgcttgatg gtggttccct tgaatacagc ccagggtatt ttttctccat ttcctgcctt
     1561 ccatttgttt gcccgggtta ttgtttcttt gaatgaactc tcaataactt cggcaagtgt
     1621 ttcactttcc ggtgtttcct gtcgatcata taattccaac tgcacactat ctttcactaa
     1681 ttggattgtg gtataatcag tcggatacct taatgatacc ttcgcgttcc tcaattcatc
     1741 ccaaagcatt tgttcaaatg ttgtccacca aatttcataa tacgaagcag cttctgaatc
     1801 aggattgttg tagtaattcc agcttttcag aatttcataa attatttttt cttcagtatc
     1861 caacccgaat ggattcaaat agccaagcag agcaggcaac gcatccgaag cctccaggtt
     1921 aaaattatca ttctgcaacc tcatcatatc ttccgcggtg atttcgttca aagaatccaa
     1981 gatataattg attctccggt ttctgaaagc ttcgaaattg gatgccgtaa tataataggg
     2041 gtaagtattg tcagcagggt actgattggc ggaactgaca aaacctctct ccggatttaa
     2101 agtttgaata ttatgttcat tcggtataaa ctccttccac ccattgaatg atttcgatcc
     2161 atccagcaca aatcttcctt catcttttcg ccggaccggg aattttccct gcacccggat
     2221 cgcaatatcg ccattcacat ccgcaaaagc gaaattctgt gccggtgatg aataatgatc
     2281 cagggcatcc ttgtaatcat gataattttt agctttgttt aatttataaa aggtgagggc
     2341 aggatcagaa gcgtcatgcg agatccaccg gaaagctaaa ttttttcttt ccgactcagg
     2401 cctgaaactc ctgtcatata tcaccgggcc ccaattcgta taaataaccg tatcgtaaag
     2461 tgattcctga cctttaattt ttatttcctc aataactttc tgagtaggca cccaaatgtc
     2521 atccaataaa tacctgtcca tactttcatc cttgaaagta attttataat aatcaatcag
     2581 atcccgctga gcattggttt ccccccaggc aatagaatca ttccatccaa cgacgacaaa
     2641 aggagctccc ggaagggaag cacccatcgt gtttataccc ggagcattca attggataat
     2701 gtaccaaatc gatggaaggt taagttccag gtgaggatca ttgcacagga taggatggcc
     2761 gctctgagtt ttctttcctg atactgccca gttattgctg cccacattgg ggtcggcttc
     2821 cggcagcaat tctgattcat ataaaccctc aggaattgcc agtggaatat tgtccagtat
     2881 aataggatta aaatcccagc ctcccgggtt atcaactacg ggatcggaca ctccttcata
     2941 atcaggatac aataagttca gcaattcaaa accgtatagt tttaagaaat tagtattctg
     3001 aagatccttt tccgccatat tcaatgtatt cgccatactc ataaatagca gcgcagtttt
     3061 taaaggttgc cattcttcgg gttcataccc taaaagttta tattccactc ccagattttt
     3121 atacttgatg gactgaatat aacggtttac cccggctgca taactttcca atgcctcaga
     3181 ggactccgga tgttccgcta ttaaagtcat tgaatttttt gctccgaaaa ccataccctt
     3241 tcttcgctgc aaccggtcaa aatccagtgc ggccggaccc ataatttctg aaatcctgcc
     3301 cgctgacgcg tgggtagtaa tttccatctg ccataatcgt tcctttgccg ttacatatcc
     3361 ctgggcaaaa tataagtctt tattattttt agcgaaaata tgaggtatca ggagagagtc
     3421 gtaaataatg gtaacctcat ccttcaatcc ttgcaattca aaatccgcat aattttcagg
     3481 gcccgtgtaa gaattttgcc agaatccatg aaatggatca agaaatttac ccaaaggagg
     3541 tatttgagca aatttggtat ttaaggcaaa taccaggacg cccgtcagca gtaacgaaaa
     3601 aataaactta ataactttca tgccaggagt tgagtgtaaa atatagaaaa gaaatagaat
     3661 attgcatcat tcagcttgaa attatatggg atatatatac cttaattcct aagatagtga
     3721 attaaaaata tgaaggagat cctgaatcta tatcccgggg aaattgtcaa aaaggcaaaa
     3781 aaaataaaga ctttgctttt tgatgtggat ggtgtgttaa cgaatgggaa aataatttat
     3841 atgggcgatg aaagtgaaat taaagagttt catgtcaggg atggacaaat aatcaagcca
     3901 ttgcgggaag caggcatcct ggtaggaatt attacaggca gagtttcaga agcggtcttg
     3961 cggcgggttt tggagttgga gtttgatatc atcaggcagg gaatcaaaaa taagttggaa
     4021 tgctatgaaa aaataaaatc tgagaatggt ttgatagctg aggaagttgc ctttataggt
     4081 gatgattttc cagatgttga aataatggaa aactgtctcc tgggaatttg tccggcagac
     4141 gcccccgatt acataaggct tagagcggat tgggtcacaa aggcaaaggg tggggaaggt
     4201 gtggtccggg aagttgccga cttaatttta tcggcccagg gaaaatttcc tatttaattc
     4261 agatttcatc ttatttgaat tcactttcct tatttttgag aaaagagaaa tcatggaaaa
     4321 attccggaat cctgttgaag ttaataaatc gattattgtg gggagtaaaa caccggtaat
     4381 cattgccgga ccatgtgctg ccgagagcta cgaattatgc aaagaggtgg gaatggaaat
     4441 gaaacaga
//
LOCUS       DASWHX010000099         5322 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_819123, whole genome shotgun sequence.
ACCESSION   DASWHX010000099 DASWHX010000000
VERSION     DASWHX010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 5322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5322
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_819123"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..953)
                     /locus_tag="VGA21_06630"
     CDS             complement(<1..953)
                     /locus_tag="VGA21_06630"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9650215.1"
                     /translation="MIKNYLISAIRNIKRNFLFSAINILGLTVGLTFCFLIALFVANE
                     YSYEAFNPDKFSIYRLNLKQFRTGNDSRIVGLTGWETGERVRTAIPDIDEMIRIRPFG
                     NYLMKTEEKSIEVSQAVLAQDNFFTFFQIPLIKGEPNQVLMDATSVVLTEETAKALFG
                     EEDPIGKIVTLKSSFELPLKVTGVARRMKNSHINYDAVIAWNASTNHGLINDWYKYSL
                     YTYFRVNAQSVISQVAEKINVILNSGNDEDKQEAYLQSLDRIYLSSSQIEFAGSFNSG
                     NPRNIRILIIAAGFILLIACVNYVNTNTSKATKRSREVGVR"
     gene            complement(967..3345)
                     /locus_tag="VGA21_06635"
     CDS             complement(967..3345)
                     /locus_tag="VGA21_06635"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9650216.1"
                     /translation="MIGNYFRIMLRQLWRNKTYFTINLIGLTAAITCVILSVSFLFNQ
                     LSYDKFHKNGNSIYRLAKKNVSTTSGAESYDAETSGMMGPTMVNQFPEVINVIRVQPW
                     WDDVLVSYKEKSFKVSPWVFADSAFFNVFDFELTRGDKSTVLTRPLSIVLTEGLAKKL
                     FGDQDPIGNSIVGMNNLDFIVTGIAADPPKNSHIRFEALVSWSTTVPGTGPLAYDFMN
                     NWLGQTIYTYLLLEENVDANRLQSKFPDFMKRNFPERAENYLLFLQPLDDVFLHSYNF
                     LYSRFPMGSIRLLSVFLAITAAVLLIACFNYINISTAKATQRASEIGVRKVLGANRRQ
                     LVTQFLGESYMLIFLSLSLSFLLADISLPTFNELVGTQLTMSGLFSIEVISSLGIIIL
                     IVGLMAGLYPAFLLSGLQSTGMLRPSSRITSGAKARHLLTLIQFMVSIALITVSLIII
                     DQYDFMRNHELGFESEKVLVIPTDNSISNSHETFRNELLKNPDILQVSICQAAIGTGT
                     FGSTVIPVGMTEEMSTQIFRVDKNFLDTYQIPLIDGRWFNSELDTGFSIVINETFAKQ
                     AGWNNLNEMAIQDTDGPVPVIGVVKDFNYRPLNEVKVDPVLMLIVNRNFNNASVRISG
                     NNIQPTLQYLEQTFLKFETRHPFEFYFVDQWFDGQYKAQDKFMKIMSLFTALSIILAC
                     LGLYGLTSFTLSSRLKEIGIRKVLGASVTSILVTVNTRFFILLILAILVASPVAFYFG
                     QRWLADFPYRIEIGFSVFMISAISALLISGITVSIQSLMAANTNPAAVLKQE"
     gene            3538..4905
                     /locus_tag="VGA21_06640"
     CDS             3538..4905
                     /locus_tag="VGA21_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009581511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX9650217.1"
                     /translation="MKEKGKLLVVDDDAYVVLSLRMFLEEHFTTVQSLATPEQMFKEI
                     EKQSFDVIILDMNFNHGETSGRDGLKYLESILEKDPDASVIMMTAYGGVNIAVEAIKK
                     GAKDFLIKPWENERLLATVMSVFNLAKEKKNVKHLLDRERVITSTIDNQFKEIIGDSP
                     AMRNVFETIRKVAGTNAEVLLTGENGTGKELVARAIHRQSNRANEIFINVDLGSISET
                     LFESELFGHKKGAFTDAKENRIGRFEAANGGTIFLDEIGNLSPALQSKLLQVLQNRTV
                     TRLGTNDSIDIDIRVICATNMHIEKMVFEGKFREDLLYRINTVEINIPKLIERREDIP
                     LLVKHFFEMYRRKYNKPNLSVSESGIKKLSSYNWPGNVREMQHALERAVIMSEGEILQ
                     GNDFEFLFGPRTSSNPEDYNLEKLEEWAIRKAMEKHSGNISHASRELGLSRGAMYRRM
                     EKYGI"
     gene            4895..>5322
                     /locus_tag="VGA21_06645"
     CDS             4895..>5322
                     /locus_tag="VGA21_06645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650218.1"
                     /translation="MASRNFKLHIFFRVIVLACSISTFLYFLLVYVNYLRSAYLLVLI
                     IAIIAEFLYYINKTNRDLSNFLTGILQNDYSTRFSEKGKGKSFNRLYQTLNRINQQFK
                     KISSEKEAQFLYLETLVEHIKIGIISYNNKGEIHLLNQAF"
BASE COUNT         1685 a         1011 c         1085 g         1541 t
ORIGIN      
        1 tttcttactc ctacttcacg ggatctttta gtagcttttg aagtattggt gtttacataa
       61 tttacacaag ctataagcag aataaatccg gcggctataa tcagaattct aatatttcgg
      121 ggatttccgg aattgaatga accggcaaat tcaatttgtg atgaactaag ataaattcta
      181 tccagagact ggagatacgc ttcctgtttg tcttcatcat tgcctgaatt taatatcaca
      241 ttgattttct ctgcaacctg ggagataacg gattgagcat tcactctgaa atatgtataa
      301 agcgaatatt tataccagtc attaatcagg ccgtgatttg ttgaagcatt ccaggcaata
      361 accgcatcgt agttgatatg tgaatttttc attcggcgcg caactccggt aacttttaat
      421 ggcaattcaa agctgctctt cagggttaca attttcccaa ttggatcttc ctctccaaac
      481 aatgctttag cggtttcttc tgtaagtacg acggatgtag catccatcaa aacctggttt
      541 ggctcccctt tgattaaggg aatttgaaaa aaggtgaaga aattatcctg agccagcacc
      601 gcctgagaaa cttcgatact tttttcttct gttttcatca ggtaatttcc aaatggcctg
      661 attcgaatca tctcgtcaat atcaggtatc gcggttctaa cccgctcccc ggtttcccag
      721 cctgttaatc cgacaattct ggaatcattg ccagttctga attgttttaa attcaacctg
      781 taaattgaga atttgtcagg attgaatgcc tcgtaggaat actcattggc gacaaaaagg
      841 gcaatcagga aacaaaaagt aaggccgacg gtcagcccta gaatattaat cgctgaaaac
      901 aagaagttcc gtttaatatt tctgatggca ctaatcaggt aattcttaat catttttcct
      961 ggaattttat tcctgtttta aaacggcggc aggattagtg ttcgctgcca ttaaagattg
     1021 aatgctgacc gttattcctg aaataagcaa agccgatatg gcgctaatca taaaaactga
     1081 aaacccaatt tctattctat aaggaaaatc agcaagccat cgttgtccaa agtaaaaagc
     1141 gacgggtgac gccaccagaa tggcaagtat cagcaatata aaaaatcttg tattcaccgt
     1201 gacaagaatg ctggtaactg aagcacccag cactttgcgg attccaattt ctttaagtcg
     1261 gcttgacaga gtaaaagaag ttagaccata caacccaaga caagccagga tgatactaag
     1321 ggctgtgaac aatgacatga tcttcataaa tttatcctga gccttgtatt gtccgtcaaa
     1381 ccattgatcg acaaagtaaa attcaaaggg atgcctggtt tcgaatttga ggaaggtctg
     1441 ttccaaatat tgaagtgtcg gctggatatt atttccggaa atccttactg aggcattatt
     1501 aaagtttctg tttacgatga gcatcagtac cgggtcaacc ttaacttcat taagaggccg
     1561 gtaattaaaa tcttttacaa ctccaatcac aggaaccggg ccatccgtat cctggatggc
     1621 catttcattt agattattcc agccagcctg tttggcaaaa gtttcgttaa ttacaattga
     1681 aaatccagta tctaattctg agttgaacca cctgccgtca atgagtggta tctggtaggt
     1741 atctaaaaaa tttttatcta cccggaatat ttgagtactc atttcttctg tcatccctac
     1801 aggaatcacg gttgaaccaa atgttccggt accaatagca gcctggcaaa ttgacacttg
     1861 cagtatatcg ggatttttca acaattcatt gcggaatgtc tcatgagagt tggaaataga
     1921 attgtccgtt ggaataacca aaactttttc agattcaaac cccagctcgt gatttcgcat
     1981 aaaatcatac tgatcaatta ttattagtga aacagtaatg agtgcgattg aaaccatgaa
     2041 ttgaattaat gttaataaat gtcttgcttt tgcacctgag gttatcctgg aagaaggcct
     2101 gagcattccc gttgattgta atcccgataa aaggaaagcg ggatataagc cggccatcaa
     2161 gccgacgata agaattatta ttcctaagga tgaaataact tcaatactaa acaaacctga
     2221 catggttaat tgagttccca caagctcatt aaaagttggt agagagatgt cagccaaaag
     2281 gaatgaaaga cttaacgaaa gaaaaatcaa catataagat tcacctaaaa attgggtaac
     2341 cagttgcctt cggtttgcac cgagtacttt ccgaactcct atttctgaag ctctttgagt
     2401 agctttggca gtgctgatat ttatataatt aaagcaggcg attaaaagta cagcggcagt
     2461 gatcgccaaa aagactgaca ggagccggat gcttcccatt ggaaagcggg agtaaagaaa
     2521 attataggag tggaggaata cgtcatccag gggttgcagg aatagcaaat aattttcagc
     2581 ccgctcaggg aaatttctct tcataaaatc gggaaacttc gactgaaggc gatttgcatc
     2641 cacattttct tccaataaca ggtaggtgta aattgtctga ccaagccagt tattcataaa
     2701 gtcatacgcc aggggtccgg ttccgggtac cgtagtagac caggatacca aagcttcaaa
     2761 ccggatgtgt gaattcttcg gtgggtcggc tgctattccg gtgacgataa aatccagatt
     2821 attcatccca acaatgctgt tgccaatggg atcttgatca ccaaataatt tctttgccag
     2881 gccttcagtt aatacaattg ataacggtcg ggtcagcaca gtagacttgt caccgcgtgt
     2941 aagttcaaaa tcaaaaacat taaaaaacgc cgagtctgca aatacccagg gtgaaacctt
     3001 aaaactcttt tccttgtagg agaccaatac atcatcccac cagggctgca ctcttattac
     3061 attgatcact tccggaaatt gattcaccat ggttggaccc atcatccccg aagtttccgc
     3121 atcataactt tccgctccgg aagtcgtact tacatttttc ttggcaagcc gatagattga
     3181 atttccattc ttatgaaatt tgtcataaga aagttgattg aacaggaatg aaacagacaa
     3241 gataacgcat gtaatagcag ctgtaagacc aatgaggttt atggtaaagt aagttttgtt
     3301 cctccataac tgacgcagca taatcctgaa ataatttcca atcatttgag tttttattgc
     3361 attttatcca attgaagtgc caaaaaagat atatgattga tcttcaatac attacattta
     3421 tttttttcgt caattttgag gttatgctgc ccacttttag aaaattaaat gcccattatt
     3481 gggcactaat gcgtctgaat tcgtatacct tggcgaaccc aagatcgccc gtaatcaatg
     3541 aaagagaaag gtaaacttct ggtagtagat gatgatgctt atgtagtctt atctctgagg
     3601 atgtttctgg aagaacattt tactacggtt caatccctgg ctacaccgga gcaaatgttt
     3661 aaggaaattg aaaagcaatc ttttgatgtg attatcctgg atatgaattt taatcatgga
     3721 gaaacctcgg gcagagatgg attaaaatat ctggaatcca ttttggaaaa agatcccgat
     3781 gcttctgtaa taatgatgac cgcttacggc ggtgtaaata tcgcggtgga ggctataaag
     3841 aaaggagcca aagactttct tattaagccg tgggagaatg aaagattatt agcaacagta
     3901 atgtcggtgt ttaacctggc aaaagaaaag aagaatgtaa aacatctgct ggatcgagaa
     3961 cgggttatta cttctactat tgataatcag ttcaaggaaa tcattggaga ttctccagcc
     4021 atgaggaatg tctttgagac tatcagaaaa gttgccggca ctaatgccga ggttttgcta
     4081 acgggcgaaa atggaaccgg taaagagctg gttgcccggg ctatacatcg gcaaagcaat
     4141 cgggccaatg aaatatttat taatgttgac ctgggttcca tatcagaaac gctgtttgaa
     4201 agtgagttat ttggtcacaa aaaaggggca tttacggatg ccaaagagaa caggattgga
     4261 agatttgagg ccgcgaatgg gggtaccatc ttcctggatg aaattgggaa tttgtcgccc
     4321 gcgcttcaat ctaaattact ccaggtgtta caaaaccgga ccgtcaccag attaggcacc
     4381 aatgactcta tcgatattga tatcagggtg atttgcgcaa ctaacatgca cattgaaaaa
     4441 atggtttttg aaggaaaatt ccgtgaagat ttgctatatc gtataaatac tgtagaaata
     4501 aatataccta agctaatcga aagacgtgaa gatattccat tgctggttaa gcatttcttt
     4561 gaaatgtatc gcagaaaata taacaaacct aatctgagtg tttctgaatc aggcattaaa
     4621 aaattgtctt cctacaattg gccgggaaat gtaagggaaa tgcaacatgc attggagcga
     4681 gcggttataa tgtcggaagg cgaaatactg caaggaaatg attttgaatt tttgttcgga
     4741 ccaaggacca gttccaatcc cgaagattat aacctggaga aactggagga atgggccatc
     4801 cgcaaagcca tggaaaagca tagcggtaat attagtcatg cgtcccggga attaggatta
     4861 tcacgaggtg cgatgtacag aagaatggaa aaatatggca tctagaaatt tcaaattaca
     4921 tattttcttc cgtgttattg tgcttgcctg ctctatttcc acgttcctgt attttctttt
     4981 ggtttatgtg aattatttaa gaagcgctta cctcctggta ttaattattg ccattattgc
     5041 agagtttctt tattacataa ataagacaaa cagggatctt tccaatttcc ttacaggaat
     5101 tttacaaaat gattacagca cgcgcttttc agagaaaggc aaaggaaaaa gttttaaccg
     5161 gctttatcag actttaaaca gaattaatca gcaattcaag aagatcagct ccgaaaaaga
     5221 agcccagttt ctttatctgg aaacactggt ggaacatatt aaaatcggga taatcagcta
     5281 taacaacaaa ggtgaaattc atctgctaaa ccaagccttt aa
//
LOCUS       DASWHX010000100        16585 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_1185927, whole genome shotgun sequence.
ACCESSION   DASWHX010000100 DASWHX010000000
VERSION     DASWHX010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 16585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16585
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1185927"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            115..1662
                     /locus_tag="VGA21_06650"
     CDS             115..1662
                     /locus_tag="VGA21_06650"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX9650219.1"
                     /translation="MSSIKLENLSIRNWKSFSSTTIQKLKQINVLIGENSSGKTNLAN
                     AIRLLLGKGIDFGSGRGAVNLDDYYKKNKPISISAKIKIKEEKTEKIVNASIEYQAFK
                     PNIPIRKEFDKIKCKFFPVGVKRSVYEIGSQQAFDCSNETNFEVAIRKWPEIRKEFED
                     FSGFKISDNPPKNKKTFLIDLVFTDGGRPITELGSGYVQAFYFIFEIFKHGDDCNIFL
                     FEEPENHMHVDLQRKFFNYLNRLAKAKEYQFFILTHSAAFIDSSIISFNNSKVYRVFK
                     TAQNTSKVEPINSEDSKWRLITEELGYKPSDLLLSNTIIWVEGPTDVIYIKRWIREWL
                     SKDENSLNKIVEGRDYTFMFYGGILLSNLAYSSNETTDEDINNFVDLCLINRNVALIL
                     DSDRKSSKQPLVSWKNKMLKRFDQEKQTDLHIWTTNCLEIENYLSKETFVKAYKKIYK
                     RKKVPPYGQFKKLTYNDYKKKETKNFKKKKFSEVAISEPVDFGVWELGKKVNDLIKWI
                     SKRALKS"
     gene            1858..2736
                     /locus_tag="VGA21_06655"
     CDS             1858..2736
                     /locus_tag="VGA21_06655"
                     /inference="COORDINATES: protein motif:HMM:NF017020.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HEX9650220.1"
                     /translation="MKRSIDHLPEEKQVELQELVRIINEQVPAEMIILFGSFARGDWV
                     DDRYKEEGTTYEYRSDFDILVVVKLEKKAKLKNFNRQWRRKILRNTAHETPVNVIFHG
                     IDYLNQEIEDGSYFFLDILQEGILLYDSGKYPLATPKALDPERRRMKAQKYYDKWLES
                     ANMFLLDFQTNFNRGKEQESHFKQAVFMLHQAAECFYLCTLLVHTDYKPKLHDLEELD
                     RQACRIDDRFKAAFPRTNPEEERLFNLLKKAYIDSRYKLEYSIASNDLEKLSASVARL
                     REITEISCRDKMDSMR"
     gene            3105..3473
                     /locus_tag="VGA21_06660"
     CDS             3105..3473
                     /locus_tag="VGA21_06660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650221.1"
                     /translation="MISEFEWIDNNAHVTFGADLSLADINQADEKIFGDLRFDLMKYA
                     IFDFSNIQKLNITKEEAQIIGILDRSSNIWYRSIRIAIITTNIELMELSEVYRTNLMG
                     SKWTVRIFIDFEAALKWCNS"
     gene            3935..4228
                     /locus_tag="VGA21_06665"
     CDS             3935..4228
                     /locus_tag="VGA21_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020602071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX9650222.1"
                     /translation="MKTKKQILFPKHQKILEQFGENIKLARKRRKMTAIQVAERAGIA
                     RNTLYLIEKGNSSVSIGAVFNVLRVLGLHEDFLKLAADDALGRKLQDLKLLGG"
     gene            4234..5484
                     /locus_tag="VGA21_06670"
     CDS             4234..5484
                     /locus_tag="VGA21_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015267917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA domain-containing protein"
                     /protein_id="HEX9650223.1"
                     /translation="MKTEKTDIFVYAHWAGMADPKLIGMLSAHRGKGRKSFSFEYDTN
                     WINSEAQFLLDPDIQWFQGPQFPANKENFGVFLDSMPDTWGRTLMKRHATQKAKDEEK
                     QVPILYDIDFLLGVYDPSRMGALRFKLDPDGPFLDNNKEYPTPPWSSIRELQYGASQF
                     ETDSNAQEAAKWLAILMAPGSSLGGARPKANILDNDGHPWIAKFPSKNDTIDKGLWEC
                     LAYKLSLKAGIYMTESKVEKVMGNHHTFFTKRFDRLNHERIHFASAMTMTGNNEDTIR
                     DNPVSYLELAEFIQFSGANNEVDLEQLWRRIVFHISISNTDDHLRNHGFILEEGGWRL
                     SPAYDINPSIDKDGLSLTIDAANNALDFDLARSVGQYFQLTDPQMDVIINEVRDSVSQ
                     WKTIAQEIGISRQEQLLMEAAFRY"
     gene            5502..6032
                     /locus_tag="VGA21_06675"
     CDS             5502..6032
                     /locus_tag="VGA21_06675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650224.1"
                     /translation="MPIHLYQSKKVQRYIGQRVYDDLPWAEEQVDPLTVWYADVFFIQ
                     RERYLILANPFTKFTFFIFQYSRKTHPDFMQTFVEKLSTTLRAADIDSTKYIEQCNLL
                     IPFNRTNKSVSAHLSQTKMDYEYMISNRWHGVHPSADEAYYNQMIADNLVSFNKKDFD
                     RPAKRFYHELILRRWD"
     gene            complement(6389..6670)
                     /locus_tag="VGA21_06680"
     CDS             complement(6389..6670)
                     /locus_tag="VGA21_06680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650225.1"
                     /translation="MERVVYNILILICISSIVNYALGQLKERNITVSWKLEVDSIEEF
                     GVYVLQRGRSYLKTKGTDGSIRESNVRFFIVWSKTGDSYKVLNDIFTRL"
     gene            7302..7433
                     /locus_tag="VGA21_06685"
     CDS             7302..7433
                     /locus_tag="VGA21_06685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650226.1"
                     /translation="MIKNYILIAWRNFTRHRTFRVGVNNVRLATTIPKKKYRKLRWK"
     gene            7810..8640
                     /locus_tag="VGA21_06690"
     CDS             7810..8640
                     /locus_tag="VGA21_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013453224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9650227.1"
                     /translation="MIDIKNLNFSYSRKQEALFNNLYCTLPNGRIVGLLGKNGAGKTS
                     LLKIMIGLLYPLEGQVEVNGYDPASRKPAFLQDVYFVSEEFQIPAISINDYVRANAGF
                     YPRFNRDLFNQLVSDFELPETRNITKFSYGQKKKFLISFSLATKCRLLVLDEPTNGLD
                     IPSKSVFRKVLAGSLDEEQLVIISTHQVKDVENLIDRILLLEDGKFIMQKDLYEISSQ
                     LNFTTSSTGEGSEILYSEMVPGGYKVITPKVNGETSVDIELLFNAITKGNQKVQKYVS
                     "
     gene            8630..9424
                     /locus_tag="VGA21_06695"
     CDS             8630..9424
                     /locus_tag="VGA21_06695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650228.1"
                     /translation="MSANDTFNINRFLLLFKQHYIHGNKIMLYSVIAFCGAAFLVLSL
                     VQLENETPLPLDTEVFITVLAGLVSVFGIIFVGHAFPAFRSKETAASYLTLPASIFEK
                     YLFELIYRVGATLIVLPVLFWITFHFHGYFFQLFTDLTFEPVNISEINKYLSIEIDED
                     SWILLMVTSMSLLAITIPFAGAAHFAKQPMVKTLFTVGILVLFFVLLIYFVFVELALE
                     NYHPNESMFLIPTNESGAFKFLSTVFVATNVFMLVIAYLKLKEKEV"
     gene            9426..9791
                     /locus_tag="VGA21_06700"
     CDS             9426..9791
                     /locus_tag="VGA21_06700"
                     /inference="COORDINATES: protein motif:HMM:NF012610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX9650229.1"
                     /translation="MEFQNGKSIFLQIADTITDKVISGEFQAGTKIPSVRELAAEMGV
                     NPNTIMRTYSELQSMQIIENKRGIGYFVNPEAPRIILENKRQEFFEKILPEFVRHAEV
                     LGIPSNDLKKHIENFNSAK"
     gene            9788..10504
                     /locus_tag="VGA21_06705"
     CDS             9788..10504
                     /locus_tag="VGA21_06705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650230.1"
                     /translation="MKFSNRLLIGFVGFVLIYMIVAFTELRLKGDNENLLSENTISET
                     MPLGNYKYLIISDLNRMISVRSSRTPGVEIKSMKGGLLSSLKYRIAGDTLALTGLEAE
                     GIRYEVCIFVPETGFNQIEIQNTNITLENLNLSEITVRQINANVSLAPDVDISKIFIK
                     ASENSNFTSREAQIDTISVNLQSSYAYLYFREALKADNKQVYRIEGSLTNSSRLIMYG
                     SGEIYIKKDATSSISWIEVN"
     gene            10617..11507
                     /locus_tag="VGA21_06710"
     CDS             10617..11507
                     /locus_tag="VGA21_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013453220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9650231.1"
                     /translation="MQIEIKGLSKVYPNGHAAIRDINLSIGSGMFGLLGPNGAGKSSL
                     MRILVTLQRSTEGSVHMDGMEIDANRNAIREQIGYLPQDFTYFSKLRTWEFLDYSARL
                     GSRMGKKERLKEVDRLLELVGLLEVRERMANKLSGGMKRRLGIAQALIGKPRLLVIDE
                     PTTGLDPEERIRFRNILSDLSQQDVTIILSTHIVGDISSTCQSMAMLHKGELAYYGSP
                     EGMISQVRGHVWEVTVDDQEFHQLKNKFPVISTVPSGISWETQLIALEKPHPDAVSIN
                     PNLEHAYVFFMENKLGISLV"
     gene            11504..14839
                     /locus_tag="VGA21_06715"
     CDS             11504..14839
                     /locus_tag="VGA21_06715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650232.1"
                     /translation="MISLRNILTISKYESRVLWRNWFFRIFALAALFFLTIFNIAGFS
                     PVGDNRWWMISNAWGIPYASMIILSIGQSAAVIFLATGLIKKDKKLDTNEVFMVRAIS
                     NTDHVFGKALALLKLFLTLNIVILCIGLTINIINPDARFNPLAYLIYPLLTSVPSIIF
                     VTGLAFLLVTVLRNQPISIVLLVGMSGIILIYFFDKYSNIFDYMAFRFSLMASDMVGF
                     NNVKMIIMQRSFYLVAGITCLFATSLFLQRLHNSRKNRIVTIILTLLLVAGSSFIMRN
                     LWKAHINTINFRKNLIAINDKWIDILNIDIESNAIEMEHFDGKIKASSRLQVRNSNSE
                     PINQIYFTLNPGLEIEEVVLNGSPIEFSRELQIISFTIERGLKVGEPAAIIIKYKGII
                     LDDVAHLDVDQKRYDRIQDYFVYSVHKQFAFLSKDYALFTKDVLWYPDTQIGFSRKSP
                     VKERKSFINFELDVKVTGNNVAVSQGILQKTAEGHFQFRPEYPLPQISLAIGSYETKE
                     LEVDSIRYIIYHFPGNDFFMKNFNQLQDTLSLLITDLVNEYEFDQQIKYPFPRLQFVE
                     VPVQFSAFDKIYEGHQAYVQPEMILYPEEGGSVRAFDFIRQMENMDEQAREENLVLSE
                     KEKQANVFKDMVKRVFTKQISDQYFYDGRESDDPNYSLFANLYAYNSGITSTEWPLFN
                     KSIAHYLNNESPAINDYSRNINGTSFTEECNAMMREATIMEILTEETDYNKIQKSVSL
                     KGDYLFSLMSQLIGEKTFKDFVYDWIGKHPHSLTSFSDFSAEIKKEFELDINPIIRQV
                     YFTTDQPSFEISNTREVELLDGDRTRYQILFDITNTAQVDGIVKINFRYWEENEIPFY
                     GEQVESEDDDLEGYYSVIKKKQSKQFGFLLDNPPDMISINTLISQNIPSIVGINPGKF
                     NRQENYTPFVGDRIVDFVTSSAQYEVIVDNEDTGFSTFSPIKETYLQQFLESRYPKNQ
                     KYYGSWRRSYSKWLATTGSGFYGNYIRSAHFTRSGKGDKITKWEPDLSESGFYDLYVY
                     MIGKNQNQYLGRDNDRKYSYEYVINHRDGTDEISFGLSNAERGWNFLGSYYFRKGEGN
                     VILTDKCDMSTVYADAVRWVRQQ"
     gene            14864..16339
                     /locus_tag="VGA21_06720"
     CDS             14864..16339
                     /locus_tag="VGA21_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013453218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX9650233.1"
                     /translation="MAVKKIKFLIVALLLLVYLFPNTARSQRVLTLEESIQIAEAGSP
                     DIRKSALNLESNKMSLVAQRASLKSRFSLDITPFDFSRTRVFNDFFSTWNTNEDYSSF
                     GRLSISQPIPITDGVLSLVDQFGYRDNYSQVQDIRTKTWSNNIYIQFEQPIFTYNRTK
                     LQLKELELNLENALLSYALQLLNLERAVTQTFYNFYQAQNSVDISRDEFNNHQLSYEI
                     TKNKVEAGLLAEVELYQAELNLATSRSTVENNQVNLDNIADDFKLLLGMDLKEDIRVQ
                     VDVEYITQNVDINQAIQHGLDERMELRQRQIDIERSEFELIRTNALNEFKGSVSLSLG
                     LFGDDSNFTEVYNSPTNNPRVAVTFSIPLFDWGEKKARMAATNANLQISRIDEENQNN
                     DIVINIRKTYRSLQNLETQIEIAEQNVKNAELTYDINQERYRNGDLTSMDLNLFQNQL
                     SDKKTQLATALINYKIELLNLKILTLYDFEKQEPVLPKTDL"
     gene            16350..>16585
                     /locus_tag="VGA21_06725"
     CDS             16350..>16585
                     /locus_tag="VGA21_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013453217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter periplasmic adaptor subunit"
                     /protein_id="HEX9650234.1"
                     /translation="MKKYSQFLFIAILALAISSCNNEESRVQTEIKIPVSVESLKPRS
                     ISKYIETTGTVYSSQEGVMKSELAGLFMLQLNPST"
BASE COUNT         5401 a         3157 c         3319 g         4708 t
ORIGIN      
        1 cctcttagca gtatatgtat tataaatatg tgtaaattca atattcacca tatgcctctg
       61 aaatccaatg gggacatgtg tgtgccttaa gcaaaattca aactgaatta tcgaatgtca
      121 tcaataaaac ttgagaacct atccataagg aattggaaat ccttttcatc aacaacaatc
      181 cagaagctaa agcaaataaa tgtattaatt ggggagaatt catccggaaa aactaacctt
      241 gcaaatgcca taagattatt gctgggtaag ggaattgatt ttggttcagg gagaggagcg
      301 gtgaatctgg atgactatta caaaaagaac aaaccaattt caataagcgc gaagattaag
      361 attaaggagg aaaaaacgga gaagatagtc aatgcctcaa ttgaatatca agcatttaaa
      421 ccaaatattc caatcagaaa ggaatttgat aaaataaagt gcaaattttt tccagttgga
      481 gtgaaacggt ctgtatatga aataggaagt caacaagctt ttgattgtag taatgaaaca
      541 aattttgaag tagccattag aaaatggcct gaaataagaa aagagtttga agatttctcc
      601 ggattcaaga tttctgataa tcctcctaag aataaaaaaa ccttcttaat tgacttagta
      661 tttaccgacg gaggtcgacc tataacagaa ctgggaagcg gatatgttca agctttttat
      721 ttcatttttg aaatatttaa gcatggtgat gattgcaata tttttctatt tgaagaacct
      781 gagaatcata tgcatgtaga tctccaacgg aaattcttta attacctcaa cagattagct
      841 aaagcaaaag aataccaatt ctttatatta actcattctg cagcttttat tgattcctca
      901 atcatttcat tcaacaattc taaagtttat agagttttta agactgcaca aaatactagt
      961 aaagtagaac cgattaattc cgaggattca aagtggagat taataacaga agagctaggt
     1021 tataagccaa gtgacctcct tcttagtaat acgattattt gggttgaagg acccacagat
     1081 gttatctata taaaaaggtg gataagagaa tggctatcaa aagatgaaaa ttcattgaat
     1141 aagattgtag agggaaggga ttatacattt atgttttatg gaggaatttt attgagcaat
     1201 ctagcatata gcagtaatga aactacagat gaagatataa acaattttgt tgatctttgt
     1261 ttaatcaaca ggaacgtggc actcatatta gatagcgata gaaaaagctc aaagcaacct
     1321 ctggtctctt ggaaaaacaa aatgcttaag agattcgatc aggaaaaaca aacagactta
     1381 catatctgga ccacaaattg tcttgaaatc gaaaattatt taagtaaaga aacctttgtt
     1441 aaggcttata agaagattta taaaagaaaa aaggtacctc cttatggtca atttaagaaa
     1501 cttacttata atgactacaa gaaaaaggaa acgaaaaatt ttaaaaagaa gaaattttca
     1561 gaagttgcaa tatcagaacc tgttgacttt ggagtttggg aattaggtaa aaaggtaaat
     1621 gatttgatta aatggatctc aaaaagagca ttgaaatcct aaacttatca aaactgccca
     1681 cgatatccgg caggcgttat ttcatcatgg agaaataacg ggtgaaatta ccaccgagga
     1741 tttgctgggg aatatcttta gcaagttttg cattggaaag tagtaagatg ataccctaat
     1801 acagtgtagt ttgctttact tcaaatttcg tactttagcg caaaaataat ttctgaattg
     1861 aagcgatcca ttgatcattt gccggaggaa aaacaagtcg aactgcaaga attagtccgc
     1921 attattaatg aacaggtgcc ggccgagatg atcatcctct ttggttcatt tgctcgcggc
     1981 gactgggtgg acgaccgcta taaagaagaa ggaaccactt atgagtaccg aagtgatttt
     2041 gatatcctgg tagtagtgaa gctggagaag aaagcaaaat tgaaaaactt caaccgacag
     2101 tggcgccgga agattctacg aaatacagcg catgaaacac cggtgaatgt gatcttccac
     2161 ggcatcgatt acctgaacca ggaaattgaa gatggcagct acttcttcct ggatatcctg
     2221 caggaaggaa ttctgcttta cgacagcggc aaatatccgt tggcaactcc caaagcactt
     2281 gatccagagc ggaggagaat gaaagcacaa aagtactatg ataaatggct ggaaagtgca
     2341 aatatgtttt tacttgattt tcaaaccaac ttcaataggg gaaaagaaca agaaagccat
     2401 tttaagcaag cagtgtttat gcttcaccag gcagcggagt gtttttacct atgcaccctg
     2461 ttggttcata ccgattataa acccaaactg cacgacctcg aagaactgga ccgtcaggcc
     2521 tgccgcattg acgaccgctt taaagcagct ttcccacgca ccaatcctga agaagaaagg
     2581 ctattcaacc tgctaaaaaa agcctacata gactcccgct ataagctgga atattccatt
     2641 gccagcaatg acctggaaaa actttccgcc tctgtagcac gcctgcgtga gatcacggaa
     2701 atttcttgca gggacaagat ggattcgatg aggtaggaga acagttttca attttcaatt
     2761 atcaatgtga ggtgtcctag atagagttga ccgtttgttg ggaactgcgc cagcagccgc
     2821 aagaaatgct tttactcaca ttatggaaaa tatatacgct gaagtcacaa ggcgtcagac
     2881 ggattgataa gtaattttat gctttctttg ttgagaggcc atttcataac agaagcagta
     2941 tgtagaatag tatccgtctg acgcctatgt gcttccctaa tggttgaaca tgggtcctta
     3001 agaatttgtc gaaatatttt tgatacaaac ctttataact cttgtcggac ggcttagatt
     3061 tacacacagt agcataagta gtttcataaa gagtaaaagc acctatgatt tctgaatttg
     3121 aatggataga taataatgcc catgtaactt ttggtgcaga tttaagctta gctgatataa
     3181 atcaagcgga tgaaaaaatc ttcggtgatt taaggtttga tttgatgaaa tatgcaattt
     3241 ttgattttag taatattcaa aagctaaaca ttaccaaaga agaggctcaa ataattggaa
     3301 tcttagacag gagttcaaac atatggtata ggagtattag aatagcgata attaccacaa
     3361 acattgaatt gatggaattg tcagaagttt atagaactaa tttaatgggt tcaaaatgga
     3421 ctgtaagaat ttttatagat tttgaagccg cgctaaaatg gtgtaactct tgaaattcaa
     3481 ctgcacataa taacggctca tctcctgagc gaattaaaga cacgccttta acaaataact
     3541 acctccaaag aaatgaccac acctgatgtc atcaaccagc gttaaaccgt taaactccgg
     3601 ttgacaagta tgtgtcacac attttgtatt agaatgtaag cgcatatcgt tcgccaactc
     3661 aaactactac ccaaaacgtt cacgacattc gtcacgcgtt atttcatctt ggggagataa
     3721 cgagggaaat taccaccgag gatttgctgg agaatatctt tagcaagttt tgtatcggga
     3781 agtgagaaac agtgaagcag cgaggcattg aaccgtctcc acaatccaac cagttaaaag
     3841 ctcagttttt ttgaactgaa ctctctcaaa aaaaacctaa tatattacta tatttcgtac
     3901 attaaatata tatttgtact acatatagta cataatgaaa acaaagaaac aaatattgtt
     3961 tcccaagcat caaaaaatac tggaacaatt tggtgagaac attaagttgg ccagaaagag
     4021 gcggaagatg acagccattc aggtggctga acgggcaggt atcgccagga atacgctcta
     4081 cctgattgaa aaaggcaatt ccagcgtctc cattggggct gtatttaatg tacttagagt
     4141 gctcggcctc catgaggatt ttctcaaatt agctgcggat gatgcattgg gcaggaagtt
     4201 acaggactta aaactgctgg gggggtaaat agcatgaaaa cggaaaagac tgacatattc
     4261 gtgtatgccc attgggcagg tatggcggac ccaaaattga ttggaatgct ttcagctcac
     4321 agaggcaaag gaagaaaatc attcagtttt gaatacgaca ccaactggat caactcggaa
     4381 gcacagtttt tactggatcc agacattcag tggtttcaag ggccacaatt tccggccaac
     4441 aaagagaatt ttggtgtttt cctggactcc atgcccgata cctggggacg cacgttaatg
     4501 aaaagacatg caactcagaa agcgaaggat gaggaaaagc aagttcctat attatacgat
     4561 attgactttc tgttgggagt gtatgatcca agcagaatgg gtgcccttcg atttaagtta
     4621 gacccggacg ggccttttct ggataacaat aaggaatacc ctaccccacc ctggtcatcc
     4681 atccgggaat tgcaatatgg agcaagccag ttcgaaacag attcaaatgc tcaggaagca
     4741 gcaaaatggc tggccattct catggcgccc ggttcttcat tgggtggcgc ccgtcctaaa
     4801 gcgaacatac tggataacga tggacacccc tggatagcca agttcccttc caaaaacgat
     4861 acgattgata aagggctatg ggaatgcctc gcttataaat tatcattgaa agcaggcatc
     4921 tacatgacag aatcaaaagt ggaaaaagtg atggggaacc accatacttt ttttacgaag
     4981 cgatttgacc ggctgaatca tgaaaggatt cattttgcct cagctatgac catgaccggt
     5041 aacaatgagg atacgataag agacaatcct gtcagctacc tggagctagc tgaatttatt
     5101 cagttctccg gggcaaataa tgaagttgat ctcgaacagc tctggcgcag gatagttttt
     5161 catatctcca tttccaatac agacgatcat ttgcgaaacc acggctttat tttggaggaa
     5221 ggaggctggc gattgtcgcc agcctatgac atcaacccct cgattgacaa agacggcctg
     5281 tcacttacaa tagatgcggc taataatgca ctggattttg atctggccag aagtgtaggt
     5341 cagtattttc agcttacaga cccacaaatg gatgtaatca ttaatgaagt cagggattcg
     5401 gtaagccaat ggaaaaccat agcgcaggaa atcggaatat cgcgacaaga acagctgctt
     5461 atggaagccg catttaggta ttgagaaaat gaaaatttaa catgcccatt cacttatatc
     5521 aatcaaagaa agttcaacga tacataggtc agcgagttta tgacgatctt ccttgggcgg
     5581 aagaacaggt ggatccactg accgtatggt atgcagatgt atttttcatt caacgtgaaa
     5641 gatatctcat actagctaat ccttttacca agtttacctt cttcattttt caatattcga
     5701 gaaagacaca tcctgatttt atgcaaacct ttgttgaaaa actatccact accctccgtg
     5761 ctgctgacat tgattcaacg aagtatatag agcaatgtaa ccttttgatc ccttttaaca
     5821 ggactaataa atctgtatcc gcccacttgt cccaaaccaa aatggattat gaatatatga
     5881 tttcaaaccg ttggcacggg gtgcatccat ctgcagatga agcatactac aaccaaatga
     5941 tagctgataa tcttgtgagt ttcaataaaa aggattttga tagacctgcc aaacggtttt
     6001 atcatgagct catcttgaga agatgggatt agaaaccaat tgaactatag aatagccttt
     6061 aatcgtatcg gtgtgatgaa agccaaatga caaaaatttg tatcgtaccc tataccctat
     6121 ttgtacccta aacacataac tacctgaaaa tcagaacttc ttacatcttg tattggaaag
     6181 tagccagcca aaacaagcaa ttatcaacaa ctaagaaaaa cagtaaaggg tcctcagaat
     6241 gggctttttt atgcctttaa agtacctttt tgctttcgtt ttgatacctg tttatttata
     6301 aagtcggtgt accggcggct tattttattc gcaagaggct tttttttcgc gacttgcata
     6361 tcgggcccat tggcttcttc gatttaactc ataaccgggt aaaaatatca ttcaaaactt
     6421 tataggaatc ccctgtttta ctccagacga taaaaaacct gacattggat tcccttattg
     6481 agccatctgt gccttttgtc tttaagtagg acctgccacg ctgtaaaaca tagacgccaa
     6541 attcttcaat agaatcaact tccagtttcc aggaaacagt gatattcctt tcctttagct
     6601 gacctaatgc atagtttact attgaagaaa tgcatatcaa tatcaagatg ttataaacaa
     6661 ccctctccat tatagtaatt aagtttaaag atttgtttcg tatttgtcaa aatccattcg
     6721 agttaattaa ggtttagttt cataattaag tttccacata atatatacat aatatagatc
     6781 atagatgagc cataaaaaat aaacggaagc aagccaagcc gacaatttat cacttacaga
     6841 aagaacaagg attaatgcca atagtaaaat cccgaaagga aaaattatca tataaaacca
     6901 ctttagtgag cgatacttga aaatgataaa atttgaaagg tccgttaatc accatcacgg
     6961 acgctaacaa taagatggat gttgaaaaaa cgggctctgt gtgaaattgc cggtaggtaa
     7021 taattccgaa tagtataata aaccgcacca acaaagtacc aggcctgaag agaaagggaa
     7081 tacttaggtc gtcttagatt atcaaaccat ttcttcccac tcttagtata tgaaatagct
     7141 tttatagtta tggagattaa tcagaggcta tcgttttaag taatgcgtac caatccatct
     7201 ttagttgttt tattttcttt ttagaaactt ccttagtctc cacgccaaat gaataatttt
     7261 tccaagccag aaatgaataa catgacttaa atactaatac catgattaag aattacatcc
     7321 tcatcgcctg gagaaacttc accaggcatc gcactttcag ggttggcgta aataatgttc
     7381 gtttggcaac aactatacct aagaaaaagt acaggaaatt gcgctggaaa taaagcaggc
     7441 acaaaaaagt ctgttctaaa gcgaacaaag acgtccgaag atgggaaaat atggaaaccc
     7501 tcaaaaggaa cttagctggg tcagctgacg actttttgat tagaatggcc ttgcagggga
     7561 tagttgcaga taacctttgt taaatccttt gaatctcact tccttttaac cgatcataca
     7621 aaattctgtg aaatccctga atccctttaa ttctgtgatc ctaactgctc ggggaaataa
     7681 cggggtaaat taccaccaaa gattccggat gtaccattta taaattttct tgaaaatttt
     7741 ctgaacggtt ttttggcttt tagtgaaaat ttgttctatt gttgtactgt attagtataa
     7801 tagtacagta tgattgacat aaaaaatctt aatttcagct attccaggaa gcaggaggcc
     7861 ctttttaata acctctattg caccttaccc aatggcagga tagtaggtct tttgggaaaa
     7921 aatggcgccg gcaaaaccag ccttctcaaa ataatgattg gattattata tccattggag
     7981 ggacaagtgg aagtaaacgg gtatgatccg gcaagccgga agccggcctt tttgcaggat
     8041 gtctattttg tttctgaaga atttcagatt cctgccattt caataaacga ctacgttcgg
     8101 gcaaatgccg gattctatcc aagatttaat cgtgacctat tcaaccaact ggtatctgat
     8161 tttgaattgc cggaaaccag gaacatcact aaattttctt atggtcagaa aaagaaattt
     8221 ctgatttctt tttcactggc cacaaaatgc cggctcctgg ttcttgatga acctacaaac
     8281 ggactggata ttccgtccaa atcagttttc agaaaagttc ttgccggatc cctggatgaa
     8341 gaacagctgg ttattatttc aactcaccag gtaaaagatg tagaaaatct gattgacagg
     8401 atactattgc tggaagatgg aaagtttatc atgcaaaaag atctatacga aatttcatcc
     8461 caattgaatt tcacaacaag ttctacaggt gaaggtagcg aaattttata tagtgaaatg
     8521 gttccgggag gatacaaggt aattaccccc aaggtcaacg gtgaaacttc agttgatatt
     8581 gaattattgt ttaatgccat cactaagggc aaccaaaaag tgcaaaagta tgtcagctaa
     8641 cgatacattc aatatcaacc gtttcctgtt attgttcaag cagcattata tccacggcaa
     8701 caaaataatg ttgtattcag taattgcttt ttgtggagct gcctttcttg tactctccct
     8761 ggttcaattg gaaaatgaaa cacccctacc cctggatact gaagtattca taacagtctt
     8821 ggccggttta gtatcggttt tcggaattat ttttgttggc catgcatttc ctgcatttcg
     8881 atctaaggag acagcggcaa gttatctcac cttaccggct tcaatatttg aaaaatacct
     8941 tttcgaacta atctatcgcg ttggggcaac cctaatagtc ctgccagttc tattctggat
     9001 cacctttcat tttcatggat acttctttca attgtttacc gatttaacct ttgagccggt
     9061 aaacatttct gaaatcaata aatacttgtc aattgaaatt gatgaagata gttggattct
     9121 cctgatggta acatctatgt ctttgttagc cattactata ccatttgcag gagctgctca
     9181 ctttgcaaag caaccaatgg ttaagactct atttacagtt ggcatactgg ttttgttttt
     9241 tgttctactc atttacttcg tttttgttga gctggccctt gagaattatc atcccaatga
     9301 gtctatgttt ctcattccga caaatgaatc cggggctttc aagtttttat ccaccgtttt
     9361 tgtcgccaca aatgttttta tgttggtcat tgcctatttg aagttaaagg aaaaggaggt
     9421 ttaggatgga gtttcaaaat ggtaagagca tttttcttca aatagcggac actattactg
     9481 acaaagttat cagcggagaa ttccaggccg gcactaaaat tccttctgtc agagaacttg
     9541 cagcagaaat gggcgtaaat ccaaatacaa taatgagaac atatagtgaa ctgcaatcca
     9601 tgcaaattat tgaaaacaaa aggggcattg ggtattttgt aaatcctgaa gctcccagga
     9661 ttatcctgga aaacaagagg caggaatttt ttgaaaaaat acttcctgag tttgtccgtc
     9721 atgcagaagt attgggaata ccctctaatg atcttaaaaa gcacattgaa aattttaatt
     9781 cagcaaaatg aaatttagca atagactatt gattggattc gttggattcg tactaatcta
     9841 catgattgtt gcttttaccg agctgcgact taaaggtgat aatgaaaatc tcctaagtga
     9901 gaatactata tctgaaacga tgccactggg aaattacaaa tatttgatta tctcggattt
     9961 gaacagaatg atttccgtga gatcatcccg tactccaggg gttgaaataa aaagcatgaa
    10021 aggtggactg ctatcaagtc tcaaatacag gattgcaggc gacaccctgg ctcttaccgg
    10081 acttgaggca gagggcatca ggtatgaggt ttgcattttt gttccggaaa ccggattcaa
    10141 ccaaatagaa atccaaaaca cgaatatcac cctggagaat cttaatcttt ctgaaattac
    10201 tgtcaggcag attaatgcaa atgtatccct tgcacctgat gtagatatca gcaagatttt
    10261 tataaaggct tcagagaatt ccaactttac ctccagggag gcgcaaatag atacgatttc
    10321 agtgaatcta cagtcgtcgt atgcttactt gtattttcgt gaagctttga aagcggataa
    10381 caaacaggtc tatcgaattg aggggtcgtt gacgaattct tctcgtctta ttatgtatgg
    10441 ctccggtgaa atatatataa aaaaggatgc aaccagctca ataagctgga tagaagtcaa
    10501 ctaagttcaa ggaccaaaat ttgaatcaaa gtgtaacaaa attgataaaa acaagctctt
    10561 agataaacca tgtccagttt tattaaaaga cattccaatc caagttcctt cgattcatgc
    10621 aaatagaaat taaaggactt tcaaaagttt atcctaacgg acatgctgct atccgggata
    10681 ttaatcttag catcgggagc ggaatgttcg gattgttggg accaaacggt gccggaaagt
    10741 cgagccttat gcggatcctg gtgactttgc agcgctcaac agaagggtca gttcacatgg
    10801 atggaatgga aatcgatgca aaccgcaatg caattcggga acaaatcggc tatctcccgc
    10861 aggatttcac ctacttctcc aaattaagaa cctgggagtt cctggattat tctgccaggc
    10921 ttggctcgcg catgggcaaa aaagaaagat tgaaagaagt tgatcgcctg ctggagttag
    10981 ttggtttgct cgaggttagg gaacgaatgg ccaataagct ttcaggtgga atgaaacgaa
    11041 ggcttggaat tgcccaggca ttaatcggga agcccaggct tcttgttatc gatgaaccaa
    11101 caactgggtt ggatcccgaa gaacgaatcc ggttcagaaa cattctttca gacttgagtc
    11161 agcaggatgt aactataatt ctttccacac acattgttgg agatataagc agtacatgtc
    11221 agagtatggc aatgctgcac aaaggtgaac ttgcgtatta tggatcgcct gaagggatga
    11281 taagccaggt acgcgggcac gtttgggagg tcacagtaga tgaccaggaa tttcatcaat
    11341 taaaaaataa gttcccggta atctcaacag ttccatcggg aattagctgg gagacacaat
    11401 tgatcgccct ggaaaaaccg caccctgatg cagtctccat taatccaaac ctggagcatg
    11461 catatgtttt ttttatggag aacaaattag gtatctcact ggtatgattt cactgcggaa
    11521 catactcacc atatccaaat atgaatccag ggtactatgg cgcaactggt ttttcagaat
    11581 ttttgcacta gctgcacttt tcttcctcac aatattcaat attgccggct tctctcctgt
    11641 cggtgacaac cgctggtgga tgatttcgaa tgcctggggt attccatatg ccagcatgat
    11701 cattctgagc attggtcagt ctgcagccgt tatttttctg gcaaccggct taataaaaaa
    11761 agacaagaag cttgacacaa acgaagtatt tatggtgcgc gctatttcca atactgacca
    11821 tgtgttcggc aaggctttgg cgctattaaa gttattccta acactaaata tcgtgattct
    11881 ttgcattggt cttacaataa atattattaa cccggatgcc cggttcaatc cgttggcata
    11941 tctgatctat cctctattga ccagtgtgcc aagtatcatt ttcgtaacgg ggctcgcatt
    12001 tctattagta actgttttga gaaatcaacc aataagcatt gtattgttgg tgggaatgtc
    12061 agggattatc ctgatttact tctttgataa atattcgaac atttttgatt atatggcatt
    12121 caggttttcg ctgatggcat ccgatatggt aggttttaat aatgtaaaga tgattatcat
    12181 gcagcgatca ttttacctgg tcgccggtat tacctgcctg tttgcaacct ccttgtttct
    12241 ccaaagactt cacaattcac gaaagaacag aattgtaaca atcattttaa ctcttctact
    12301 tgttgctggt tcatcgttca tcatgaggaa tttgtggaag gcgcacatca acaccattaa
    12361 ctttcgcaaa aaccttatcg ctattaatga caagtggatc gatatcctca atattgatat
    12421 cgaatctaat gcaatcgaaa tggagcactt tgacggcaaa atcaaagcct cttcgagatt
    12481 gcaagtacgg aattcaaatt ctgaaccaat aaaccaaatc tacttcacct taaaccccgg
    12541 ccttgaaatt gaagaagttg tcttaaatgg ttcacccatt gaatttagcc gagaattaca
    12601 aataatttcc tttacaattg agcgcggact caaagttggc gagccggcgg cgataataat
    12661 caaatacaaa ggaattattc ttgatgatgt ggctcacctg gatgtggatc agaagcggta
    12721 cgaccggatc caggattatt ttgtatactc ggttcacaaa cagtttgcat ttctcagcaa
    12781 ggattatgcc ctctttacaa aagatgtttt atggtatccg gatacccaaa tcgggttttc
    12841 gcggaaatcg ccggtgaaag agagaaaatc atttatcaat tttgaactcg atgtgaaggt
    12901 aacagggaac aatgttgcag tttcccaggg aattttgcaa aagacagcgg aaggtcattt
    12961 tcaattcagg ccggaatatc ctttgccaca aatttcattg gcaattggtt cctacgaaac
    13021 caaggaactt gaagttgaca gcatacggta tattatatac cattttcccg gcaatgactt
    13081 cttcatgaag aattttaacc aactacagga tacacttagt ttgctaataa cagatttggt
    13141 taatgaatat gaatttgatc agcaaataaa atatccattc cccagactgc aatttgtaga
    13201 ggttccagtt cagttcagtg ctttcgacaa aatttacgaa ggtcaccagg cttacgttca
    13261 acccgaaatg atcctctatc ctgaagaagg tggcagtgtc cgcgcatttg acttcatcag
    13321 gcaaatggaa aatatggatg agcaggcaag ggaagaaaac ctggttctta gtgaaaaaga
    13381 aaagcaggca aatgttttca aagacatggt taaaagggtt ttcaccaaac aaatctctga
    13441 tcaatacttc tatgatggcc gcgaatccga tgatcccaat tactctttgt ttgcaaacct
    13501 atacgcatat aattcaggca taaccagtac cgaatggccg ctttttaata aaagtattgc
    13561 ccattatctc aacaacgaaa gccccgcaat caacgactat tccaggaata tcaatggcac
    13621 ttcttttaca gaagagtgca atgcaatgat gagggaagca actataatgg aaatcctgac
    13681 tgaagaaact gactacaaca aaattcagaa atctgtatca ctcaaagggg actacctgtt
    13741 ttccctaatg agtcagctta ttggagaaaa gactttcaag gattttgttt atgattggat
    13801 tggtaaacat ccgcattcgc taaccagctt ttctgatttc agcgctgaaa taaagaagga
    13861 gtttgaattg gatataaatc caataatccg gcaggtctat ttcacaacag atcaaccttc
    13921 gtttgaaatt agcaatacca gggaagtgga gctgctggat ggcgaccgta ctcgttatca
    13981 aatcctgttt gatattacaa acaccgcgca agttgatgga attgtgaaaa taaactttcg
    14041 ctattgggag gaaaacgaga taccgtttta tggagaacag gtagaaagtg aagatgatga
    14101 tctggaaggc tattattcag taatcaaaaa aaaacagtcc aaacaattcg gattcctgtt
    14161 ggacaatcct ccggatatga tttccataaa taccctgatt tcgcagaata ttccttcgat
    14221 tgtcggcatt aatccgggca aattcaatcg ccaggaaaat tatacccctt ttgtcggaga
    14281 tcgaattgtt gattttgtaa cctcatcggc gcaatatgag gttatcgtcg acaatgaaga
    14341 taccggattt tcaactttca gccctatcaa ggaaacctac ctgcagcaat tcctggagag
    14401 caggtatcca aagaaccaaa aatattatgg tagctggcga cgctcctatt caaagtggct
    14461 ggccacaact ggatcaggat tttacggaaa ctatattcga tctgctcact ttacacggtc
    14521 aggtaaaggc gataaaataa ccaagtggga gccagaccta agcgaaagtg gattttatga
    14581 tctgtatgtt tatatgatag gcaaaaatca aaatcaatac ctcggaagag ataatgaccg
    14641 gaaatacagc tatgaatatg tgataaatca tagggatgga accgatgaaa tcagctttgg
    14701 cttatcaaat gctgagcgag gctggaattt ccttggttca tattatttcc gaaaaggcga
    14761 gggtaatgta attctgactg ataaatgcga tatgagtact gtttatgctg atgcggtcag
    14821 gtgggtaaga caacaataaa ttcaccatat aatatgtaag aaaatggcag tcaaaaaaat
    14881 aaaatttcta atcgttgcat tactgctttt ggtgtacctg tttcctaata ctgcaaggag
    14941 tcagcgggtt ctcacccttg aagagtcaat tcaaattgct gaagccggaa gcccggacat
    15001 cagaaaatct gctctgaatc ttgagagcaa taaaatgagc cttgttgccc aacgagcaag
    15061 tttaaagtca agattttctt tagacattac gcccttcgac ttcagccgca cacgcgtttt
    15121 caatgatttt ttctccacat ggaataccaa tgaagactac agtagttttg gacgactttc
    15181 tatctcacaa cccattccca tcactgatgg agttctttcc ctggttgacc aattcggata
    15241 cagggataat tatagtcagg tccaggatat caggacaaag acctggagca acaatattta
    15301 tatacagttt gaacaaccca ttttcactta caaccgcacc aagctacagc ttaaggagct
    15361 ggaattaaat ctcgagaatg cactgcttag ctatgccctg caattactta acctggaacg
    15421 tgcggtaact caaacatttt ataattttta ccaggcacag aatagcgttg atatttccag
    15481 ggacgaattc aataatcacc agctcagtta tgagatcaca aaaaacaaag ttgaagcggg
    15541 acttcttgct gaagtagaac tttaccaggc agaactgaac ctggccacgt caagatccac
    15601 agtagaaaac aaccaggtta acctggataa cattgccgat gatttcaagc tgctcctggg
    15661 catggatctt aaagaggaca tcagggtaca ggtggatgtt gaatatataa cgcaaaatgt
    15721 tgatatcaac caggcaattc aacacgggct tgatgaacgc atggaactaa gacaacgaca
    15781 gattgatatt gagcgatctg agtttgaatt gatacgcacc aatgctttga atgaatttaa
    15841 aggatcagtt tcactttcac ttggactctt cggtgatgat tcaaatttta cggaggtgta
    15901 taattcacct accaacaatc cgcgagtagc tgttaccttt agcattccgc tttttgactg
    15961 gggcgaaaaa aaagcccgga tggccgccac caatgcaaac ctgcaaattt caagaattga
    16021 tgaagaaaat cagaacaatg atatcgtcat taatatcagg aaaacctacc ggagccttca
    16081 aaacctggaa acacaaattg aaattgcaga gcaaaatgta aagaatgcgg aactgacgta
    16141 cgacattaat caggaaagat atcgcaacgg ggaccttacc agcatggatc ttaacctttt
    16201 ccagaaccaa ctgtctgata aaaagacaca gttagctact gcgctcatta attataaaat
    16261 tgaactgtta aacctgaaaa tacttacgct ttatgatttc gaaaaacagg agcccgtact
    16321 gcccaagaca gacttataat ttattcgcta tgaaaaagta ttcacaattt ctatttattg
    16381 ctattctcgc attggcgatc tcatcatgta ataatgaaga gtcaagagta caaactgaaa
    16441 ttaaaattcc cgtgtcggtt gaaagcctga agccgcgatc aatatcaaaa tatattgaaa
    16501 caaccggtac cgtatattcc tcccaggaag gtgtgatgaa atctgagtta gcggggttat
    16561 ttatgctcca gctcaatcct tcaac
//
LOCUS       DASWHX010000101         7930 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_1189455, whole genome shotgun sequence.
ACCESSION   DASWHX010000101 DASWHX010000000
VERSION     DASWHX010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 7930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7930
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1189455"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(515..805)
                     /locus_tag="VGA21_06730"
     CDS             complement(515..805)
                     /locus_tag="VGA21_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014018495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HEX9650235.1"
                     /translation="MRIIVDYNDAKSGFSQLKIDSAKYLSDYAIRIKFNDGNERLVDF
                     KPFLSKSLHPSIKKYLDENKFSNFSLTDGNLNWNDYDLIFPISDLYNGKIDA"
     gene            complement(802..1119)
                     /locus_tag="VGA21_06735"
     CDS             complement(802..1119)
                     /locus_tag="VGA21_06735"
                     /inference="COORDINATES: protein motif:HMM:NF025088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4160 domain-containing protein"
                     /protein_id="HEX9650236.1"
                     /translation="MIDNAKDLFEYLGILIFFYSNEREPIHVHAKKGEFESKAEFYII
                     NGEISEIKITSISGARPLKGKDLKDFKVFLEMYPDKIVEKWISYFVYHKDVEFEKITK
                     RLK"
     gene            complement(1465..2310)
                     /locus_tag="VGA21_06740"
     CDS             complement(1465..2310)
                     /locus_tag="VGA21_06740"
                     /inference="COORDINATES: protein motif:HMM:NF020419.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HEX9650237.1"
                     /translation="MIQPGEIQSIAGKEGVRDTQIEKNYVISWVTYGIANNPFLKENL
                     VFKGGTVLKKVYFPDYRFSEDLDFTFTPAHRSNSLGGGKAFDIEAIKAAFNKLIEWVY
                     EESRISLSVQNETQHDTGNYNFYFSYTGPLGGAGTNKDIKVDISQDELIYNAPEEKQI
                     INTYSDLEEEYSIFCYSLGEVIAEKMRSIMQRTAPRDIYDLWYLFETEGQDIEDHIFA
                     FQDKAKHKGYEPNELIKVIEQKEKTFAKHWKDHLANQMTEIPDFNDVWRELGKHWRKF
                     QKFIK"
     gene            complement(2451..3269)
                     /locus_tag="VGA21_06745"
     CDS             complement(2451..3269)
                     /locus_tag="VGA21_06745"
                     /inference="COORDINATES: protein motif:HMM:NF020962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin"
                     /protein_id="HEX9650238.1"
                     /translation="MKKNISYRSAEFLDALSKRKQDFFTLKEAVEMLPDKDYATVRKL
                     LSDMTKRGLIMRIKDGLYHRIPYEQQPDQYFPNWHLTAEAIAQPREYYIGFYSALDIH
                     GLITQPSMTEQVVTWERVKPKTQQVKNVRFEFITLSKSRFFGYKKQWIDNFHKVNCSD
                     LEKTFLDCLYKPDYAGGITEITQALYKSREKLQSARFQEYLEKFNIQAVYKRLGFIVN
                     ELGLFQELQDFITEKISSSYTPLDPSFDKHGRYNSKWGIIDNIGFGEVIEGLGT"
     gene            complement(3536..4441)
                     /locus_tag="VGA21_06750"
     CDS             complement(3536..4441)
                     /locus_tag="VGA21_06750"
                     /inference="COORDINATES: protein motif:HMM:NF019421.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA domain-containing protein"
                     /protein_id="HEX9650239.1"
                     /translation="MDKNEIDYHAKCVMKTFGLKQMPTIDIDEKKLTAYAKKIVGAST
                     AITGVQPKLSLWLDESKKSVRFTVVDEKSNYIIKPQSEVYQSLPENEDLCMHLAETFG
                     IETATHGLIRLPGGELAYITQRFDRKNENKFSCEDLCQLSETLTEHKYRGSYEKTGKV
                     IKQYSTQSGLDTLHYFELVVFSFIVGNADMHLKNFSMFEKQEGQFSLSPSYDLVSTML
                     VIKNESEQMALTLNGRKNKITKKDFTALAINLSITEKQVENSFHSFSEKLKSAQWWIE
                     NSFLPEEQKADLNKIVTDRISLLSK"
     gene            complement(4470..4787)
                     /locus_tag="VGA21_06755"
     CDS             complement(4470..4787)
                     /locus_tag="VGA21_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017729669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA N-terminal domain-containing protein"
                     /protein_id="HEX9650240.1"
                     /translation="MKKGWVYKKEILSGIVWEDENGYHFQYEKSYLQNPVYGAVSRTL
                     PLRSEQFTDKNMLPFFDGLIPEGWLLQIAIDNWKLNPRDRMTLLLTLCKDCIGDISIR
                     SEK"
     gene            complement(5055..5222)
                     /locus_tag="VGA21_06760"
                     /pseudo
     CDS             complement(5055..5222)
                     /locus_tag="VGA21_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009578570.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(5414..5581)
                     /locus_tag="VGA21_06765"
     CDS             complement(5414..5581)
                     /locus_tag="VGA21_06765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650241.1"
                     /translation="MIRKIGMRYNPQITPVIDTNKDFVSGEVPPTSENSSGFSDWCVL
                     ARLITNPRNYS"
     gene            5991..7493
                     /locus_tag="VGA21_06770"
     CDS             5991..7493
                     /locus_tag="VGA21_06770"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX9650242.1"
                     /translation="MKNYILLTFLLTQGVTFGGYSQEISGKVQGRVYDSENKDIYYIE
                     PLEGKYFESNLKKAALSSAGLFQIEIYSSKPAFIYFFSPGNFDLLHMVFYKPGMKSTM
                     NFRDNKILGFKGDYTTENEFLSGLQRSYSHSPNLTFINSLVKNKTPANAYEEWSQYFE
                     NETANLENYFNDTHCDNDLQEFLLVDHKAFLVNSFITYIIKQSPVGFSQLTRSQMDDS
                     QKMEVNTYVADWGNILEKVFSHLSLDKNAYYSKDYNVYVDNYIGWYLLRFRGINQPSN
                     FVDLVDWHYNYSPKFESHLPPEAQEAWLARHLQFLRGLGSIAYDVGFINLQNYFRNKY
                     PESAYLDHLNNFSIDVYKYLNSPASLNDYKIFLSSQYETLESLYENIENKFIYVDLWA
                     TWCKPCIEEFQFYPNLRNQLKTDSVEFLFISVDDSASRQKWQEMVHRFDLNGVHIMAN
                     SNLRAYIWNKIIKEKTEAYPRYLLIAPDGKIMDANAPPPSNAEKLIQLIK"
BASE COUNT         2315 a         1531 c         1448 g         2636 t
ORIGIN      
        1 cgaagatttt tccacccata catttcagat tgaaatttca ggctcgggga aatgtgaggt
       61 aaatgtttca caaacacttg atgcagacat tagcggcagt ggctccgtct attacaaagg
      121 aaatccggaa aaagtacgca gtaacatatc tggcagcgga cgggttcgga gaatataatt
      181 atagaaggtt tcattgcctg ccatgtcaac taccgtaatt taaagtccat aagaaaatcc
      241 gatagcctgc cctatggttc tactttagaa atcaaatgca gaaatacaga tgctggtcat
      301 gattaccgat gaaacgggtc agcacacgcg gggcatcaaa ctcgccaaca aatgcatgat
      361 gagtgccgcc gttgcctaca acccttaaaa agatgatgaa atttaatgca gcaaaccgat
      421 agcggcgatg aatatacacg aaaagttgaa gtgggctatc ccattctcat actgaaaccc
      481 ttattggcta taccgcgtgt tgtggtttcg tagtttatgc atctatcttt ccattatata
      541 aatctgaaat tgggaaaatc aaatcgtaat cattccagtt taaattacca tcagtcaatg
      601 aaaaattcga gaatttattt tcatccagat atttttttat cgatggatga agtgatttag
      661 ataaaaatgg tttgaaatca acaagtcttt cgtttccatc gttaaacttt atccgaattg
      721 cataatctga taaatatttt gcagaatcaa ttttcaactg gctaaatcca gactttgcat
      781 cattatagtc aacaattatt ctcattttaa tcgttttgtg attttttcaa attcaacatc
      841 tttgtgatac acaaaatagc taatccattt ttcaactatt ttgtcaggat acatttcaag
      901 aaataccttg aaatccttta ggtcttttcc tttaagaggt cgagctccag aaatacttgt
      961 aatctttatc tctgaaatct cgccatttat gatataaaac tccgctttac tttcaaactc
     1021 tccttttttt gcgtgaacat gaatcggttc gcgctcattt gagtagaaga aaatcagaat
     1081 tcctaagtat tcaaataaat ctttggcatt atcaatcagt taatattttg aactattaca
     1141 aaattataaa atctaaatct gaactgaaaa tcattgtttg taattgggtt cctcaccatg
     1201 aaccacaatt gccttggcta agaaacgtgc gggttttgga gataggtatc tgtcagccga
     1261 gtaacgatgt ttattatgtg tttttaatat tcattttacc ttgtcagccc gcatgatttc
     1321 ttagcctttg ttggcaactg ttgcccgcac ctccaatgtt tattttgttc cccaaatctg
     1381 ttttcctttt ttgtcgcgcg tggggggagg ttttttgttt tctttttcat tttatacgat
     1441 gcgctggcgg tttacagcgt ttgcctattt aataaatttc tgaaattttc tccaatgttt
     1501 tcctaattcc cgccatacat cattaaagtc cggtatttct gtcatttggt ttgccaggtg
     1561 atctttccag tgttttgcaa aggtcttttc cttttgttca atcactttga tcaattcgtt
     1621 gggttcatag cctttatgct ttgccttgtc ctgaaaggcg aaaatgtgat cttcaatgtc
     1681 ctgtccttca gtttcaaaca agtaccagag gtcgtaaata tctctgggag cagttcgctg
     1741 cataatagaa cgcatctttt ccgcaatcac ttctccgagt gagtagcaga atatgctgta
     1801 ttcttcttca aggtcggaat aggtgtttat gatctgcttt tcttccggtg cgttgtagat
     1861 cagttcgtcc tgactgatgt caactttgat gtctttattg gttccggctc cgcccaatgg
     1921 cccggtataa gaaaaataga agttgtaatt cccggtatca tgttgtgttt cattctgaac
     1981 cgaaagcgaa atccgggatt cctcatacac ccattcgatt aatttgttga aagcagcttt
     2041 tatggcttca atatcaaagg cttttccccc acctaagcta ttgctacggt gggcaggtgt
     2101 gaatgtaaaa tcaaggtctt ctgaaaaacg gtagtccgga aaatatactt ttttcaaaac
     2161 cgtaccgcct ttgaaaacca ggttctcttt caggaatgga ttatttgcta taccatacgt
     2221 cacccaggaa atgacatagt ttttttcaat ttgggtatcc cgaacgcctt cctttccggc
     2281 tatggattgt atttctcctg gttgtatcat ttttatgttt cggtaactgg gtgattaagg
     2341 taactgagta atcgggtaaa tgaggtaatt gaggagccta actcccattt acctcaaccc
     2401 ctcagttacc ttaattacca ttttacccaa ttccatattt acttaattac ctatgtaccc
     2461 aacccctcaa tcacctcacc aaaaccaata ttatctataa tcccccattt tgaattatac
     2521 cgaccatgct tgtcaaatga cggatccaat ggggtgtagg atgaagaaat cttttcggta
     2581 ataaaatcct gaagttcttg aaacagacca agttcattaa cgataaaacc aagtcgcttg
     2641 taaacggcct gtatattgaa cttttcaaga tactcctgaa atcgtgctga ctgaagcttt
     2701 tccctggatt tgtacagcgc ctgagttatt tccgtgatgc ctccggcata atccggttta
     2761 tacagacagt ccagaaatgt cttttcaaga tcgctgcagt ttactttgtg aaaattatca
     2821 atccactgct ttttatagcc aaagaatcgg cttttgctca aagtaataaa ctcaaaccgg
     2881 acatttttaa cctgctgtgt tttaggtttc accctttccc aggttaccac ctgctcagtc
     2941 atagatggct gagtaatcaa accatgaata tccaatgcac tgtaaaaacc tatgtaatat
     3001 tcccttggtt gtgctattgc ttctgctgtt aaatgccagt tgggaaaata ttgatcaggt
     3061 tgttgttcat atggaatacg atggtacaac ccgtctttaa tgcgcatgat aagtcccctt
     3121 ttcgtcatgt cgcttagcag ctttcttact gtcgcataat ccttatctgg cagcatttca
     3181 accgcctctt ttagcgtaaa gaaatcctgt ttccgcttac tcaatgcatc cagaaattca
     3241 gctgatctgt aagaaatatt ctttttcatg ccttgtaagt gagtttaaaa ctcacaaatc
     3301 agacacaaat gtaagaattt aattgaaatt ggctactttg taccaatatg gctgatatac
     3361 acgaaccgga aacccggctg ccggtcgggc aggtaagaag ctaacatgag caggattaag
     3421 ggcaaggaca caaagcctta tttgttgccg gctgcccgaa tcttcctacg aagcggtgtt
     3481 ggtgcaagtt tgcaggcact ctgtttaaga tgatttataa taatatcggt tgccattatt
     3541 tactcaacaa acttattctg tctgttacga ttttatttaa gtcagctttt tgttcttcag
     3601 gcaaaaaact gttttcaatc caccattgtg ctgatttcaa tttttctgaa aacgaatgaa
     3661 aactgttttc aacttgcttt tctgttatgg aaagattaat tgctaaagcc gtaaaatctt
     3721 tttttgttat tttatttttt cttccattaa gcgttaatgc catttgttct gattcgtttt
     3781 ttattaccaa cattgtgctt accaaatcat aggaagggct taaagagaat tgtccttctt
     3841 gtttctcaaa catggaaaaa tttttcaagt gcatatctgc attgccgact atgaaactga
     3901 atactaccag ttcaaaataa tgtaaagtgt ccagtccgga ttgggtagag tattgcttaa
     3961 taactttgcc tgttttttcg taactgcctc tgtatttatg ttcagtcaat gtttcagaaa
     4021 gttgacataa atcttcacaa gaaaatttgt tttcattttt cctgtcaaac cgttgagtaa
     4081 tgtatgctaa ttctcctcct ggcaatctaa ttagaccatg cgttgctgtt tcaattccaa
     4141 atgtttcagc aaggtgcata cataaatctt cgttttcagg taacgattga tagacttcac
     4201 tttgcggttt tatgatatag ttgctttttt catcaacaac tgtgaaacga acactctttt
     4261 tactctcatc caaccacaaa cttaatttgg gttgcactcc tgttatggcg gtgcttgcac
     4321 ctacaatttt ttttgcgtag gcggtcaatt ttttttcatc aatatcaatc gttggcattt
     4381 gctttaagcc aaaggtcttc atcacacatt ttgcatgata atctatttca tttttatcca
     4441 acggtttata gcaaagtagg cagttcattt tatttttcgc ttctaatact aatatcacca
     4501 atacaatctt tacaaagtgt aagtaatagt gtcattcggt ctcgtgggtt tagcttccaa
     4561 ttatcaatag ctatttgcag taaccaccct tcgggtataa gcccgtcaaa aaaaggtagc
     4621 atatttttat ccgtaaattg ctccgaacgc aatgggagtg ttctgctgac tgctccataa
     4681 actggatttt gcaaatacga tttttcatat tgaaaatggt atccgttttc gtcttcccaa
     4741 acaattccac ttagtatttc ttttttatat acccatcctt ttttcatcgc ttactgttct
     4801 tcaattgttt gaatatcgtc atgcgaaata gtttcggtca agttatcatc tggtttttgt
     4861 tgatacttta tggcattgtt gtttggcaca aattgccatg cagaaatttt attttctttg
     4921 ttatctgtct tttcttttat gatgataccg tatttaacga tcctgtttgt tagtgttatt
     4981 tttacagctt tattaaagta gttccagaaa atatcgtatg catcaatttg ttgtttgcca
     5041 ggtaataggt taaacccgaa caaagccaac acctgattta ctttatctag ttgcacggtt
     5101 tctttgccct gctccagttc acgaataaaa cgaatgccta cacctgcttt tgcagcaagt
     5161 tcttcctgcg ttagctttag ttttttgcgt tgatacttga tgaatgatga taagttgttc
     5221 attgtgttac tataccttta cgggtgcaaa tatagacaaa acattaaaaa aagaactgaa
     5281 cgggtataaa aaatataaat tggttatttt gaatcctgaa cgggtatcgt ttaaaatgtc
     5341 ggtaagtcca ttcgttcaaa gctcaaatgt ttcaagttag cactgtccca agcattgcag
     5401 ccaacggtct cggctatgag tagttgcgtg gattggtgat taaccttgca agtacacacc
     5461 aatctgaaaa tccgctagaa ttttcagaag taggcggtac ttcccccgaa acaaagtcct
     5521 tgtttgtatc gatgactggt gtaatttgcg gattgtatcg cattccaatt tttcttatca
     5581 tctgaagata ggttttgata tgtggttgct aacgcacatt atgccgacac ctcgtggaac
     5641 acgaagtcaa tggttgggtt ctataataga cttcacaaac ggagacttct tgcaaaacaa
     5701 ctgaacaaag aatcaaagtt ggttgcgaag gactcattaa aagtgttagc tgagtttgaa
     5761 aaacttaacg atgaagatta agcaatttga gatttgcaaa acaggaactc tttctccaaa
     5821 attattccgt tacatcaggt ttccggacag tgagggcttg cgcaaaatcc agttccagtt
     5881 agaatttatt aatttaatac tgcaagactt gcttcaaaga ttttacagtg gtattttttc
     5941 ctgtgaccgc gaagacattt gaacgaagcc atgttaatta gattaataca atgaaaaatt
     6001 atattttgtt aacttttttg ctcacccagg gagtgacttt tggcggctat tcacaggaaa
     6061 tatccggcaa agtgcaggga agggtgtatg attcggagaa caaagatatt tactacattg
     6121 aaccattgga aggaaaatat tttgagtcga acttaaagaa ggctgcgcta agttctgcgg
     6181 ggttattcca aatagaaatt tatagttcga aacccgcatt tatatatttt ttttcaccgg
     6241 gaaatttcga tttgttgcat atggtttttt ataaaccggg tatgaaatcc actatgaatt
     6301 tcagagataa caagatatta ggttttaaag gggattacac gacggagaat gaatttttat
     6361 ccggattgca aagatcttat tctcattctc caaacctgac ttttataaac tccctggtaa
     6421 aaaacaaaac cccggcaaat gcttatgaag aatggagcca atattttgaa aatgaaactg
     6481 caaacctgga aaactatttt aatgataccc attgcgataa cgatctgcag gaattcttac
     6541 ttgttgatca taaggccttc ttagtaaatt cttttattac atatataatt aagcaaagcc
     6601 cggttggttt ctcacaattg accagaagtc aaatggatga ttctcaaaaa atggaagtaa
     6661 atacctatgt ggctgattgg ggaaatatac ttgagaaagt gttctctcac ctttctctgg
     6721 acaaaaacgc ttattattct aaagactaca atgtgtatgt tgataactat atcggttggt
     6781 acctgctaag atttagagga attaaccaac cttctaattt tgttgacctt gtagattggc
     6841 attacaatta ctcaccaaaa tttgaatcgc atctgcctcc ggaagcacag gaagcatggc
     6901 tggcccgcca cctgcaattc ctgagaggat taggatcgat tgcatacgat gttggtttca
     6961 ttaacctgca aaattatttc agaaataaat atccggaatc tgcttacctg gatcatctta
     7021 acaatttttc aattgatgtt tacaagtatc tcaacagccc ggcatcttta aacgattata
     7081 aaatttttct tagctcccag tatgaaacat tagaatcatt atatgagaac atcgaaaata
     7141 aatttattta tgtggacctc tgggctacct ggtgcaaacc ctgtatcgag gagtttcaat
     7201 tctaccctaa tctcaggaac caattgaaaa ctgattccgt agaatttttg tttatttctg
     7261 ttgatgattc ggccagtagg caaaaatggc aggagatggt tcatagattt gatcttaacg
     7321 gcgtccatat aatggcgaat agcaacttaa gagcttacat ttggaataaa ataattaaag
     7381 aaaagactga agcgtatccg aggtatttac taattgctcc ggatggtaaa ataatggatg
     7441 caaatgcgcc tcctcccagt aacgctgaaa agttgattca gcttattaag tgagaaggga
     7501 aggtaatttt tgttgcctcc ctctttattg ccttcgctgg gtctgcgcag cgaacccgtc
     7561 gaaaactgcc cgatgtatca tgccgcgggg tcccttggaa aacccacggt aacagaaaat
     7621 ccgttaagtc ccaaagggac gacctgtttg tagaaaataa aacaatgcaa taagcagctc
     7681 cgtaggagcg acctgtttgt agtagaaaaa atccgttaag tcccaaaggg acgacctgtt
     7741 tgtagaaaat aaaacaatgc aataaggaag ctccatagga gcgacctgaa atatgccaaa
     7801 cacctacacg caaatttata ttcagattgt ctttgcagtc aaaggcaggg aaaatcttat
     7861 ttcaaaatca caccgcgaag aattgcataa atacattacc ggcattgtgc aaaagcgtga
     7921 acaaaaaatg
//
LOCUS       DASWHX010000102        12453 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_3057673, whole genome shotgun sequence.
ACCESSION   DASWHX010000102 DASWHX010000000
VERSION     DASWHX010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 12453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12453
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3057673"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..685
                     /locus_tag="VGA21_06775"
     CDS             <1..685
                     /locus_tag="VGA21_06775"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: protein motif:HMM:TIGR00571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Dam family site-specific
                     DNA-(adenine-N6)-methyltransferase"
                     /protein_id="HEX9650243.1"
                     /translation="DVTKLLDFLYRYQKKYIKLDKKQRQEFFYDQRTNYNLQRFNIDY
                     DKYSEQWFPRAAQLIFLNKTCFNGLYRVNSTGEFNSPAGDYDNPTICDEQNLLAVHQV
                     LQIAEIKKADFKEIVTDLKAKSFVYFDPPYRPISKTASFNTYSKHHFADNQQVQLAEL
                     FKQLDKKGTQVMLSNSDPKNNDPSDNFFDDIYSDYYITRVPARRIINSDPTKRGTINE
                     IVVTNYSIA"
     gene            819..1415
                     /locus_tag="VGA21_06780"
     CDS             819..1415
                     /locus_tag="VGA21_06780"
                     /inference="COORDINATES: protein motif:HMM:NF019958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX9650244.1"
                     /translation="MLTKSERTTQFILETVAPIFNKKGYFGTSMSDLTAATGLTKGSI
                     YGNFENKEQLALEAFNYNVMKVNGLLKKHVGLHKKAKDKLFALTEFYRGYKQYTGEFG
                     GCPLINVGVDSAHQNPVLANRVKQVVHKWKESLVRIIEQGIQDGNFRKSVKPEKLANL
                     IFGMIEGGAFLTVIADDKMIMIDMMNEVDRIIERDLIK"
     gene            1476..2342
                     /locus_tag="VGA21_06785"
     CDS             1476..2342
                     /locus_tag="VGA21_06785"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9650245.1"
                     /translation="MKLIQTSTRIFFKITSLIAPAMAANIAFQLFQTTRKKVRSKERD
                     FFQNSTHFHVPGNQENIDCYELGNPKGEIVFLVHGWESNAGSMSAIAAKLASLNYRVI
                     AFNFPAHGTSNLKKANLLVCSHAFLNVLGYIKPKDRFSVVGHSFGSAVSAYTLSKVDM
                     QINKIMFLTTPNRISDIFSEFRKMIGLGESSYKLLLQKAQALLGEDPADVNVEDKMKM
                     VKFSQLLILHDLFDKVLAHKNSLNVHKAISNSRMVTTFEVGHYRMLWDEKVLDQIEEY
                     FSPGFVALPKAS"
     gene            2415..3860
                     /locus_tag="VGA21_06790"
     CDS             2415..3860
                     /locus_tag="VGA21_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020527188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008509 - monoatomic anion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006820 - monoatomic anion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HEX9650246.1"
                     /translation="MSPMTNVRKIGFILGPVLFFALLSLQEVSPLEPTAWKVLAMTAW
                     MVTWWITEAIPIPVTAILPMVLLPLLQIFNIREATASYADPIIFLFMGGFLIALGLEK
                     HHLHTRIALNLIKITGTNSNGIVLGFMLATAFLSMWISNTAATVMMLPIAMSIIELIP
                     RNQPELAKSHDFKMFALCLMLGVAYAANIGGTATIIGTPPNIVLVGFAQTMLHQDLSF
                     SKWLLIGLPFAATMLLLTFFMLTRFLYPSKMGSISGSRQVVDQKIRELGPVSTAEKRV
                     SLVFGLTALSWIFRSPLNQLIGQNILNDTIIAMTGGILMFIVPSGNGKALLSWKDSKR
                     LAWGILLLFGGGLTLAKSLEEAGIIQIIGQSIQENGSFGVLLVLFLLITVMLFMTELM
                     SNVALATIFIPVVIGIAGGLGVEPLYFAIPVTLASSCAFMMPISTPPNAIVFSSGFIR
                     MDQMVKAGVWLNLISIFILMILGSSIVRWIY"
     gene            complement(3923..4270)
                     /locus_tag="VGA21_06795"
     CDS             complement(3923..4270)
                     /locus_tag="VGA21_06795"
                     /inference="COORDINATES: protein motif:HMM:NF039701.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6249 domain-containing protein"
                     /protein_id="HEX9650247.1"
                     /translation="MEEGILAIIWLIIFSIGALIMVVYLRKFSNHEKMAMIEKGMSPG
                     DFKPEPRNSSISLRASLLLMGAGAGLLFGYFLDMNFRMQEVGYFSMLFIFGGIGLGLS
                     YLIEEKKNKEFNK"
     gene            4423..4992
                     /locus_tag="VGA21_06800"
     CDS             4423..4992
                     /locus_tag="VGA21_06800"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX9650248.1"
                     /translation="MGVQQEEYELIDRILEGEEASYKILVDKYKSYAFSLSLRILNNR
                     EDAEEASMDAFVKAFHSLKGFNRNSKFSTWLYRIVFNTSLTYKKKQKLNQSIDDTYLG
                     ETLESEETDELQEKDQRKFIMQALKELLPMDATILSLFYLKENTLEEIGEVVGMNPNS
                     VKVKLFRARKRLADELTKNLSHEAIALIK"
     gene            5012..5548
                     /locus_tag="VGA21_06805"
     CDS             5012..5548
                     /locus_tag="VGA21_06805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650249.1"
                     /translation="MEKITDQELIDLIDNRFGVERSRKMLELINHDQHLLRRYQMMKV
                     IHQHLSNSTHEEAPVSFSDRVMRNLHNPQYIQGVIAARYNSRNLFTFFTIFAGILVGV
                     YILASGIVAIPFFDQFSINPVNLQGNKIDINPIIDGLTSSVIFKVFLIADMVLAWFIL
                     DRVVLKPYFETRRKKYAY"
     gene            5517..5789
                     /locus_tag="VGA21_06810"
     CDS             5517..5789
                     /locus_tag="VGA21_06810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650250.1"
                     /translation="MRQEGKNTLIELLSGFPERQNLPGILNFPRIYSDIIFLKTQLRK
                     LAKTFLATSLFWLLSFSHKRSQMNSIAFENGDVRIATAESGCHIPH"
     gene            complement(5895..6569)
                     /locus_tag="VGA21_06815"
     CDS             complement(5895..6569)
                     /locus_tag="VGA21_06815"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HEX9650251.1"
                     /translation="MKIDRIKDAGNIFNNYQLMHNGLKIGLGSYYDYGNAILIRENRG
                     VHEPEEESAFQQIIDQIPANGCIIELGSYWAFYSMWFAMKIPNASCYMIEPDPHKMNF
                     GKINFKLNGLNGKFILGYIDNKSDLRKPIPTFSVDYLVNKHNIDQIDILHSDIQGFEF
                     RMLTGATRLLTDQRIRHLFISTHSNELHYSCIQLLESKNYLIDVSIDLDKTYSWDGLI
                     VATRPK"
     gene            6815..7753
                     /locus_tag="VGA21_06820"
     CDS             6815..7753
                     /locus_tag="VGA21_06820"
                     /inference="COORDINATES: protein motif:HMM:NF013681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-1,2-fucosyltransferase"
                     /protein_id="HEX9650252.1"
                     /translation="MISFISIIKIHISFMIIQRLRGGLGNQLFQYAAGKALAEIHDTS
                     YKLDLYYYKKHPYRTFDLENFNIPIEIATRKEVHSFTGQNPVARFLNKYENYFHCPKV
                     FVQPHYHYYNHFLKLPNNLYLSGYWQSEKYFIRIRDKVLNWFTLKHPLDKRNTDLIEK
                     MKTNQSISVHVRLGDYTHKNYNEFFGEMDSSYFSKAIQIMLDKVKDPAFYIFSEDLQW
                     CKERLNLPETSVFISHNKGRESYKDLILMSACKNNIIANSTFSWWGAWLNPNKDKVVI
                     APAKWFQQDYMTKRTPSFPTRLYINSDLYPDDWIII"
     gene            7750..8859
                     /locus_tag="VGA21_06825"
     CDS             7750..8859
                     /locus_tag="VGA21_06825"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX9650253.1"
                     /translation="MSPTEIIMIAGAVHKAFDTRIFHKEAKTLSNAGYKVSLIIPHDK
                     DELISGINICAVSRPKSGFQQLFITPLKIFWKTLSFSRKSIIHIHDSELLVIGVLLRL
                     SGRRVIYDAHEDTPRQLQYQHWIPILIKPFYILFYFLLERFCGILFNAIIVAEPVIGR
                     HYPKRKTTLIRNFVISEFFRPVEINDESKKNQIIYIGLLSKPRGLFEMVEAMKLLPQE
                     LDIKLILGGKFAPAALENEIENIPKIEFIGWVPFEQIPGLLSVSKIGIITPLPYKRYL
                     TNYPVKLFEYMASGLPVIASKFGESAKFVEEANCGLLVDPQRPEEICEAIIKLISNPD
                     RAIKMGINGQKLIFEKYNWESESEKLIAVYKNLLK"
     gene            9148..10035
                     /locus_tag="VGA21_06830"
     CDS             9148..10035
                     /locus_tag="VGA21_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009580454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HEX9650254.1"
                     /translation="MDIWQTFKDGYSGYANYLYQEITFQYDYKPWWENYFYWLLLVSL
                     FFLILEWTRPWRKEQPKFRKDFWMDFFYMFFNFFLFSLIIYNAASSVVVDVFNNLLSI
                     FGIRNLAAINIAEWNRFAQLFTLLLIRDFIQWWVHRLLHRSPWLWEFHKVHHSVEQMG
                     FAAHLRFHWMENIVYRFLEYLPLAMIGFGISDFFIVHIFTLAIGHFNHSNLKVNLGPF
                     KYIFNNPQMHIWHHAKDLPPERRYGVNFGLTFSLWDYLFKTNYIPYTGKNIPLGFPGI
                     EKFPKSFVKQNLHGFKTKK"
     gene            10045..11040
                     /gene="moaA"
                     /locus_tag="VGA21_06835"
     CDS             10045..11040
                     /gene="moaA"
                     /locus_tag="VGA21_06835"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012929150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HEX9650255.1"
                     /translation="MVESKAIYDNHGRPINYLRLAVTDRCNLRCFYCMPEEGIKYLPK
                     KELLTYEEMERVVKILSHLGINKIRITGGEPFVRQDLIKFLHRIRLNDKIEKLSITTN
                     GILTRDYIPDLKDLKISSVNLSLDTLNPDRFRKITRRDNFDKVFATLQDLMANNFEIK
                     INMVVMTNQNVEDILPMVELTREYPVNVRFIEEMPFNGEGSKYASLDWTHRKILSHIK
                     SEYPEMTKLVDDPHSTSMNYRIKNFQGSFGIIAAFSRTFCGSCNRIRITAQGIMKTCL
                     YDGGVLNVKELIRSGCDDSVIAGEFISAFKNRYKDGVEAEKNRVPIHESMSTIGG"
     gene            11395..>12453
                     /locus_tag="VGA21_06840"
     CDS             11395..>12453
                     /locus_tag="VGA21_06840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650256.1"
                     /translation="MQRLIFKKDLLPHLLAIIIFLLVTIVFFSPVYFQGKSLHQDDIY
                     MWQGGAKELMDFREETGEEGLWTNSMFSGMPGYLINTQFSGDLIYYFQRILAFGLPHP
                     TSILFTSFLCYYIMLLVFGVRPGIAIAGALAFGLSSFNIIGLAAGHNARIAAISFMPL
                     VLAGIHLCLKSRPLLGAVITSIGLAIQLRVNHIQITYYLLLVVLIYGAVFLVEAYKNK
                     GLKSFITRLGFLVLAVLLAVGANLGRLWTILEYNKYSMRGISELSRTVERGENESGLE
                     KSYAFMYSSGISESLTLFIPNYKGGPNYQSLDSDSNLGQALRNNGVAPGQINQYLQNV
                     PTYWGKQPATAPYYLGAIM"
BASE COUNT         3913 a         2279 c         2363 g         3898 t
ORIGIN      
        1 agacgtaaca aaactgcttg actttcttta tcgctaccaa aaaaaataca tcaaacttga
       61 caaaaaacaa cgccaggaat ttttctacga ccaacggaca aattacaact tgcagcgttt
      121 caatattgat tacgacaaat attccgagca atggtttccg agagcggcac aacttatttt
      181 tttaaacaag acttgtttca acggactgta tcgtgttaac tcaacaggtg agttcaattc
      241 accggctggc gactatgaca accctacaat ttgtgatgag caaaatttat tagcagttca
      301 tcaagtattg caaattgctg aaatcaaaaa agccgacttc aaagaaattg taactgactt
      361 aaaagcaaag tcattcgttt actttgatcc accttacaga ccaattagta aaactgcaag
      421 ttttaataca tacagcaaac accactttgc cgacaaccaa caagttcaat tagcagaact
      481 ttttaaacag ctggataaaa aaggaacaca ggtaatgcta agcaactccg atcctaaaaa
      541 caatgaccct tcagacaatt tttttgacga catatacagc gactactaca ttacaagagt
      601 tccggcaaga cgaatcataa attccgatcc tacaaaacgt ggaacaataa atgaaatcgt
      661 tgttaccaat tattccatag cataaatgga aaaagaaact aaaagtatcg taacaggaaa
      721 tcaacttgaa gtggcctcaa aactgtattg acaagttgat atccaattcg tataatatac
      781 cgtttggtat ttttcaatta aattgttata tttgcttcat gcttactaaa tcggagcgaa
      841 caactcaatt tatcctggaa actgtagccc ccattttcaa taaaaagggg tactttggca
      901 cgagtatgag cgaccttacg gctgcaacag gtttaaccaa aggttcaata tatggtaatt
      961 ttgaaaataa agaacaattg gcgcttgaag cctttaatta caatgtgatg aaggttaatg
     1021 gactgttgaa gaaacatgtt ggcttgcata agaaagcaaa agataaattg tttgccctga
     1081 ctgaatttta tagaggatat aagcagtata ccggtgaatt tgggggttgt ccgttgatca
     1141 atgtgggagt agacagcgca caccaaaacc cggttctggc aaatcgcgta aagcaggtag
     1201 tgcataaatg gaaagaaagt ctcgtcagga ttattgagca gggaatacag gatggtaatt
     1261 ttagaaagtc agttaagccg gaaaaattgg caaatttaat ctttggtatg attgagggtg
     1321 gagcatttct taccgttatc gcggatgata aaatgataat gattgatatg atgaatgagg
     1381 ttgaccggat tatcgaaagg gatttaatca aataattttt ttttgaatat atatataccg
     1441 attggtattt ttataagact ttaaaccatg acacaatgaa attgattcaa acctctacaa
     1501 gaattttctt caaaataact tcacttatag ctccggcaat ggcggcaaat atcgcatttc
     1561 aactattcca gacaacccgg aaaaaagttc gtagcaaaga gcgagacttc tttcaaaatt
     1621 ccacgcactt ccacgtacct ggtaaccagg aaaatattga ttgctacgaa ttgggaaacc
     1681 caaaaggtga aatagttttc ctcgtccatg ggtgggaaag caacgccggc agcatgtctg
     1741 ccattgcggc aaaactcgca tcactaaatt accgggtgat cgcctttaat tttccggctc
     1801 atggcacatc aaatctcaaa aaagcaaatt tactggtttg ttcacatgca tttctcaatg
     1861 tattaggtta cataaaacca aaagaccggt tttcggtggt cggtcattcc tttggctcag
     1921 cagtttctgc ttatacttta tctaaagttg acatgcagat taataaaatt atgttcctga
     1981 ccacacctaa ccggatttct gatattttca gtgagttccg caaaatgatt ggcttaggtg
     2041 aatcttccta caagttacta ctccaaaaag cccaggcatt attgggtgaa gatccagccg
     2101 atgtcaatgt ggaagataaa atgaaaatgg tgaagttttc ccagttgttg atactccatg
     2161 atttgttcga taaagtatta gcgcacaaaa attctttgaa cgtacataag gctatttcaa
     2221 acagcagaat ggtgaccaca tttgaggtag gccattatag aatgttgtgg gatgaaaaag
     2281 ttcttgatca aattgaagag tatttttcac ctggttttgt cgcgttacct aaagcatcct
     2341 aaggcatatt tgatccaaat actttttaag aaataatcaa ttcaatacat ttagggcgct
     2401 aaataaataa tgcaatgtcg cccatgacaa atgttcggaa aatcggattt attttgggtc
     2461 cggttttatt ttttgcattg ctgagccttc aggaagtttc gcccctggaa ccaactgcct
     2521 ggaaagtgct ggccatgaca gcctggatgg ttacctggtg gataactgaa gctatcccaa
     2581 ttccagtcac cgccatcctt ccaatggttc tgctccccct gctgcagatt tttaatatcc
     2641 gggaagctac ggcctcttat gcagacccga ttattttcct cttcatgggc ggatttctaa
     2701 tagctcttgg gcttgaaaag caccacctgc ataccagaat tgccttgaat cttataaaga
     2761 taaccgggac caattccaat ggaattgtgt taggctttat gctggccacc gctttcctga
     2821 gtatgtggat cagcaatacc gccgcgacag taatgatgct gcctattgcc atgtccataa
     2881 ttgaattaat tcccaggaat cagccagaac ttgcaaagtc gcatgatttc aaaatgtttg
     2941 cattgtgtct catgcttgga gttgcctatg cggccaatat tggtggcact gcaacaataa
     3001 taggtacgcc tccaaatatt gtccttgtcg gattcgcgca gacaatgctg caccaggatt
     3061 tgtcttttag caagtggctt ctcatcggcc tgccttttgc tgcaacaatg cttttgttaa
     3121 ccttttttat gctcaccagg tttttatacc caagtaaaat gggttctata tccggctcaa
     3181 gacaggtagt ggaccaaaaa atcagagaat tgggtccggt atcaaccgcg gaaaaaagag
     3241 taagtctggt gtttggtctt accgctctca gctggatttt cagaagtcca ttaaatcaat
     3301 tgataggaca gaatatttta aatgatacaa taatcgcaat gaccggagga atcctgatgt
     3361 tcatcgtacc ttccggtaat gggaaagctt tgctttcatg gaaagattcc aaaaggttag
     3421 cctggggcat tcttttgctt tttggagggg gattaacttt agcaaaatca ttggaagagg
     3481 cggggataat acaaattatt ggccagtcca ttcaggaaaa tggtagtttt ggagttctgt
     3541 tagtactgtt cttactaata actgtgatgt tatttatgac cgagttgatg agcaatgtcg
     3601 ccctggcaac aatttttatt cccgtggtta ttgggatagc cggcggactc ggggttgaac
     3661 cgttgtattt tgcaattcct gttactctgg cctcaagctg cgcgttcatg atgccaatct
     3721 ctacaccacc taacgcgatt gtattttcaa gcggatttat cagaatggat cagatggtca
     3781 aagcaggagt gtggctgaat ttgatttcca tttttattct gatgatttta gggtcaagta
     3841 ttgttcgatg gatttattag gcataaaaaa accccgatta aattaccggg gtctttgagc
     3901 ctgaccttaa atctgtatca atttatttat tgaattcctt atttttcttt tcttcaatca
     3961 gataggataa cccaagacct attccgccga agataaataa cattgaaaaa taccccactt
     4021 cttgcatgcg gaagttcata tccaggaaat aaccaaataa cagaccggca ccagctccca
     4081 ttaggagcaa tgaagctctc aatgaaattg aactgttccg cggctccggc ttgaaatctc
     4141 cagggctcat tcccttttca atcatcgcca ttttttcatg gtttgagaat tttctcaagt
     4201 aaacaaccat tatcagtgct cctatactga agatgattaa ccagattatt gctaatattc
     4261 cttcttccat tttattattc gttaatactt gatttgtctt tatgacttaa ccggttgcaa
     4321 ccaggttaca cccggctgag aatatttaaa gaatatgcca aatgctcctt aaattgacga
     4381 attaaactgt aacttttatc atcatcaggt tgtcaaagaa ccgtgggtgt acaacaggaa
     4441 gaatacgaat taattgatcg tatactggaa ggtgaggaag catcttataa aatacttgtt
     4501 gacaagtata aatcatatgc tttttcactt tcattacgaa ttttgaataa tcgtgaagac
     4561 gctgaggaag ccagtatgga tgcgtttgta aaagcctttc attcactaaa gggttttaac
     4621 aggaattcga agttttcaac ctggctttac aggatagtgt ttaacacttc cttaacctat
     4681 aaaaaaaaac aaaagttgaa tcaaagtatt gatgatactt atcttggtga aacgctggaa
     4741 agcgaagaaa cagatgaatt gcaagagaaa gaccaacgga aatttattat gcaggcacta
     4801 aaagaattgc ttccaatgga tgcaacaatc cttagtttgt tctatttaaa ggagaatacc
     4861 cttgaagaga ttggagaagt agtcggcatg aatccaaatt cggtaaaagt caagcttttc
     4921 agggccagaa agcgccttgc cgatgaatta acaaaaaatc tgtcgcatga agctatagcc
     4981 ttaataaaat aatgatttaa tttgagatga aatggaaaag ataacagacc aggaattaat
     5041 tgacctcata gacaataggt ttggagttga aaggtccagg aagatgctgg aacttattaa
     5101 tcatgatcag cacctgttga gacgttatca aatgatgaag gtaattcatc aacatttaag
     5161 caactcaaca catgaggaag ctccggtcag tttttctgat cgggtaatga gaaatctgca
     5221 taacccgcag tatatacaag gtgttatagc agcccggtat aattccagga atttattcac
     5281 attctttacc atctttgcag gtattttggt gggagtgtat atacttgctt caggcattgt
     5341 tgccattcca ttttttgatc aattctctat aaatccggtc aaccttcagg gaaacaaaat
     5401 cgatataaat cctatcatcg atgggcttac ttcaagcgta atttttaagg tttttttgat
     5461 agcagatatg gttctggcct ggtttatcct ggatcgcgtc gtacttaaac cttactttga
     5521 gacaagaagg aaaaaatacg cttattgaat tgctttcagg atttcctgaa cgtcagaatt
     5581 taccgggtat ccttaatttt cccagaattt attctgatat aattttcctg aaaactcaac
     5641 ttcgcaaact tgcaaaaacg tttttagcaa cttcactttt ctggcttttg tcattttccc
     5701 ataagcgttc tcaaatgaat tccatcgcat ttgaaaatgg ggatgtaagg attgcaaccg
     5761 ccgaatcagg ttgtcatatt cctcattgag tgttttattt atattcatat atttaatagt
     5821 aatatataaa taaatataat aattagatat aattaataca attacataat ggttatggat
     5881 gatgaaaata acttttattt tggtcttgta gccacaatca aaccatccca ggaataggtt
     5941 ttatctaaat ctatggaaac atcaattaaa tagttttttg attcaagtaa ttgaatgcat
     6001 gaataatgca attcattaga atgggtggaa atgaaaaggt gcctgatcct ttggtcagtc
     6061 aaaagcctgg tagcccctgt gagcattcta aattcaaatc cttgaatatc actatgtagg
     6121 atatcaattt ggtcgatatt atgtttgttg accaaataat caacactaaa tgtgggtatt
     6181 ggctttctta aatcagattt attatctatg tatcccaaaa tgaattttcc attaagtcca
     6241 tttaatttga aattaatttt cccaaaattc attttatgcg ggtctggttc aatcatatag
     6301 cagctggcat tgggtatttt catagcaaac cacatggaat aaaatgccca atagcttcct
     6361 aattcaataa tgcatccatt tgccggaatt tgatcaataa tttgttggaa agcggactcc
     6421 tcttcaggtt catggacgcc cctattttca cgaatcagaa tggcatttcc gtaatcataa
     6481 taactgccca atcctatctt aagcccatta tgcatcaatt gataattatt gaagatatta
     6541 cccgcgtcct tgattctatc aattttcata ttctccgggc attcttttac ccttgaaatt
     6601 ctatgggacc aaaattccgg tagcttatat gccggaaaga agaatctgaa ccttaaattg
     6661 aaataattaa tcctattcag gaaatacaga attgtattat aaaccaaagg cttgcttttt
     6721 aaccatagct taattctcat catcaattcc agtcaaatca aaataattat gaaatataca
     6781 gctctgtaaa ttacatttta tcaaatgtat gagaatgatt agctttatat ccatcattaa
     6841 gattcatatt tcattcatga taattcaaag gctaagaggt ggacttggta atcagttatt
     6901 tcagtatgct gctggaaaag ctttggctga aatacatgat acttcataca aactcgatct
     6961 atactattat aaaaagcatc cttacagaac attcgatttg gaaaacttca acattcccat
     7021 agaaatagcc acaaggaagg aagtccattc tttcactggc cagaatcctg tcgcaaggtt
     7081 tcttaataaa tatgagaatt attttcattg tccaaaagta tttgttcagc ctcattatca
     7141 ttattacaac catttcctta aacttccaaa taatttatac ttatcaggat attggcaaag
     7201 cgaaaagtat tttataagaa taagagacaa ggtacttaat tggtttactc taaagcatcc
     7261 cctggataaa aggaacacag atctgattga gaaaatgaaa acaaatcaaa gtataagtgt
     7321 ccatgtccgg ttaggtgatt atactcacaa gaactacaat gaatttttcg gtgaaatgga
     7381 ttcaagttat ttttcaaaag cgattcaaat aatgttagat aaagtaaagg atccggcttt
     7441 ctatattttt tcagaagatc tccagtggtg caaagaacgc cttaatctgc ctgaaacaag
     7501 tgtttttatc tctcataata aggggcgaga atcttataaa gacctcattt tgatgtctgc
     7561 atgcaaaaac aatataattg caaacagcac ttttagctgg tggggtgcct ggttgaaccc
     7621 aaacaaagac aaagttgtca tcgcaccggc aaaatggttt caacaggatt atatgacaaa
     7681 aagaacacct tcttttccca caagacttta cattaattct gatttgtatc ccgatgactg
     7741 gataattata tgagtcctac agaaattatt atgattgcag gggcagttca taaagcattt
     7801 gatacaagaa tttttcataa ggaagcaaaa actctgtcaa atgccggtta taaagttagc
     7861 cttataattc ctcatgataa agatgaattg atttcaggca taaacatatg cgcagtttca
     7921 cgtccaaaat ccggatttca acaattattt ataaccccgc tgaaaatttt ttggaaaacc
     7981 cttagcttct cccggaaatc aattattcat attcatgatt ctgaacttct ggtaatcgga
     8041 gttctcctaa gactctcagg cagaagagta atttatgatg ctcacgagga cactccccgg
     8101 caattgcaat accagcattg gatacctatt ttaataaagc cattttatat tctgttttat
     8161 tttctgttag aacggttttg cggcatttta tttaatgcta ttatagtagc tgaaccagta
     8221 attggcaggc attatccaaa acgaaaaact acacttatcc gaaattttgt aatcagcgag
     8281 ttctttcggc cggttgaaat taatgatgaa tcaaaaaaaa atcaaattat ttatattggg
     8341 ctgctaagca aaccacgagg gttatttgaa atggttgaag ccatgaaatt gcttcctcaa
     8401 gaattagata taaaacttat tttgggtgga aaatttgccc cggctgccct tgagaatgaa
     8461 attgaaaata taccaaagat tgaattcatt ggctgggttc catttgaaca aatacccggg
     8521 ctgctttcgg tttctaaaat aggaataatt actccactgc cttataagcg atacctgact
     8581 aattaccctg ttaaattatt tgaatacatg gcatccggat tgccggtgat cgcatccaaa
     8641 tttggtgagt ctgccaaatt cgttgaagag gcaaactgcg gcttattggt ggaccctcaa
     8701 cggccagaag aaatttgtga agcaattatt aagctaataa gtaatcccga tcgagctatc
     8761 aaaatgggga taaatgggca aaagttaatt tttgaaaaat acaattggga aagtgaatct
     8821 gagaaattga ttgctgttta taaaaattta ctaaaataat atcttgatat aatttaatga
     8881 actttgtaga cttttttgca aaaatttata ttcctcattg gacggtgtga gatatatgaa
     8941 gaggcgacga catcaggtga ggttcaactt atttttaaca accagcctta tgcgtaagac
     9001 ttctgctgga tataatactt ttgagttcat ttctcatcca attttccact gctcttcata
     9061 actatttgtt tatgaatact gaatttccca ctatggtctt aaatcattat ctttagaagt
     9121 ctttattaaa ttggagatgt tgaggcaatg gatatttggc agacatttaa agacggttat
     9181 tcaggctacg ccaattattt gtaccaggaa atcacctttc aatatgatta taaaccctgg
     9241 tgggaaaatt atttctactg gttattactg gtgtccctgt ttttccttat tctggaatgg
     9301 acaagaccat ggagaaaaga acaacctaaa tttcgaaaag atttttggat ggacttcttc
     9361 tatatgttct tcaatttttt tcttttttcc ctgataattt acaatgctgc ctccagcgta
     9421 gttgtagatg tcttcaataa tcttttgtca atattcggaa ttcgcaatct agctgctatt
     9481 aacattgctg aatggaaccg ttttgctcag ctatttacat tactattaat tagagatttc
     9541 atacaatggt gggtgcacag gttattacat cgttccccct ggctttggga gtttcataag
     9601 gttcatcact cggttgaaca aatgggattt gccgcccacc tgcgattcca ttggatggaa
     9661 aatatagtat accggttttt ggaatattta ccgctggcta tgattggctt tggtattagc
     9721 gactttttca ttgttcatat ctttacactt gcaattggtc attttaacca cagcaacctg
     9781 aaagttaacc tgggtccttt taaatacata ttcaataatc cgcaaatgca tatctggcat
     9841 catgcgaaag atcttccacc tgaaaggaga tatggcgtta attttggcct gacattcagt
     9901 ttgtgggact atcttttcaa aacgaattac atcccatata ccggaaaaaa catacctttg
     9961 ggattccccg ggattgaaaa gttcccaaaa tcttttgtca aacaaaatct tcacggattt
    10021 aagacaaaga aataagggca agcgatggtt gaaagtaagg caatatatga taatcacgga
    10081 aggccaatta attacctgcg gctggcagtt accgataggt gcaacctgcg ctgcttttat
    10141 tgtatgcctg aggaaggtat aaaatatctc cctaagaaag aattgcttac ctatgaagag
    10201 atggaacggg tggtcaaaat tctttcacat ctcggaatta ataagattag aattaccggt
    10261 ggcgagccat tcgtgagaca ggatttgatc aaattcctcc acaggattag actgaatgat
    10321 aagattgaaa aactttcaat aactaccaat ggaattttga caagagatta tatacctgat
    10381 ttaaaggatc tcaaaatctc atcagtcaac ttaagccttg acactttaaa tccggatcgg
    10441 ttcagaaaaa ttacaagaag agataatttc gataaagttt ttgcgacact tcaggatctg
    10501 atggcaaaca attttgaaat caagattaat atggttgtta tgaccaacca gaacgtagaa
    10561 gatattctgc ctatggttga acttacccgg gaatatccgg ttaatgtccg ctttatcgaa
    10621 gaaatgccct ttaatggtga gggatcgaaa tatgccagtt tagattggac ccatcgcaag
    10681 attctttcac acattaaaag tgaatatcct gaaatgacca aactcgttga tgatccgcac
    10741 tctacctcca tgaactaccg aattaagaat ttccagggct catttggaat tattgccgca
    10801 ttcagccgca ctttctgtgg atcatgtaat cgaatccgga taacagcgca gggaattatg
    10861 aagacgtgct tgtatgatgg tggcgttctt aatgtaaaag aactgatcag atccggttgt
    10921 gacgatagtg ttattgccgg cgaatttatt tccgcgttca aaaacaggta caaggatggt
    10981 gttgaagcag agaaaaatcg agttcccatt catgagtcaa tgtctactat aggaggttag
    11041 aatgttccgg gttttttacc acgccatttg atatttatga atccgctaat aaagagtttt
    11101 taaatttact actgaagcca aacttttaat attggcaacc tccttttgcc tgggcggatt
    11161 ggtctggtgg tgcaaacgag aaattgcccc agtccagata aataaagcga ttctctctgg
    11221 aaatatcaga cattgaaaat caatcaattt cactaaaata aaaaatggaa gtgaggcaca
    11281 cgttgatgtt aatttcattt ggatttaaac tgagacatta ccgaattgta aggcagattt
    11341 tttatcagta cattatccat tattcattat tattgtgttg attaaaaaaa aagtatgcaa
    11401 agacttatct ttaagaaaga tcttctcccc cacctgcttg ccattataat ctttttactt
    11461 gtcaccattg tcttttttag ccctgtctat tttcagggaa aatctctgca tcaggatgat
    11521 atctatatgt ggcagggagg tgctaaagaa ttaatggatt ttcgggagga aacaggtgaa
    11581 gaaggattgt ggactaatag catgtttagt ggtatgcccg gttatttaat aaatactcag
    11641 tttagcggag accttattta ttacttccaa agaattcttg cctttggatt acctcatcca
    11701 accagcattc ttttcacctc ctttttatgc tattatatta tgctgctggt gtttggtgtc
    11761 cgcccaggta ttgcaattgc gggcgcattg gcatttgggc tttccagttt caacataatt
    11821 ggacttgcag caggtcataa cgcgagaatt gcagctatat cgtttatgcc tttagtgttg
    11881 gccggaatac acttgtgttt aaaaagcagg cctttattag gcgcagtcat tacttcaatt
    11941 ggtcttgcca ttcagcttcg ggttaatcat attcagatta cctattatct tctccttgtc
    12001 gttttaatct atggagcggt tttcctggta gaagcttata aaaataaagg gttgaaatcc
    12061 ttcatcacaa ggttaggatt tcttgtacta gctgtcctcc ttgcggttgg agcaaacctc
    12121 ggaagactat ggaccattct tgagtataat aaatacagca tgagggggat ttccgagtta
    12181 agcagaactg tagaaagagg tgaaaatgaa agcggtttgg agaagtcgta tgcttttatg
    12241 tacagttccg gaatttcaga aagtcttacg cttttcattc ccaattataa gggtgggcca
    12301 aattatcagt ctctggacag cgatagtaat ttggggcagg ctttgcgaaa caacggcgtt
    12361 gctccgggtc agataaatca gtatttgcaa aatgttccta cttactgggg caaacagcct
    12421 gctactgcac cctattattt gggagcgata atg
//
LOCUS       DASWHX010000103        22913 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_3059157, whole genome shotgun sequence.
ACCESSION   DASWHX010000103 DASWHX010000000
VERSION     DASWHX010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 22913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22913
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3059157"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..646
                     /locus_tag="VGA21_06845"
     CDS             <1..646
                     /locus_tag="VGA21_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015330316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX9650257.1"
                     /translation="RLGYEVELANLTESGRSYISKGFLDEARKIAIENVSKASEFVNP
                     ENPLIGIEPSAILSFRDEYIDLVEVEKREKATNLSENCLLLEEFLSREIDAGKITSKQ
                     FTDKAETVKVHAHCHQKSLSSLTHLKKALSVPKNYQVHFLRTGCCGMAGSFGYEKEHY
                     DLSMKIGNLVLFPALKKLTSEIVAAPGTSCRHQIKDGTGMKALHPAEILLKALR"
     gene            646..2523
                     /gene="asnB"
                     /locus_tag="VGA21_06850"
     CDS             646..2523
                     /gene="asnB"
                     /locus_tag="VGA21_06850"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002704768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HEX9650258.1"
                     /translation="MCGITGIYAFNQIGRFQMINAARATEALHTRGPDFQRTINNEYA
                     SFGHRRLSIIDTSPAGNQPIEDERNWLVFNGEIYNYRQLRSELESAGESFYSHSDSEV
                     LFRLLKSRGSEGIEKLQGDFAFAFYDAAEDSVLIARDRFGVKPLYYFKDEDKFIFASE
                     IKSILSYGLDLELDHDALLIYLQLNYIPAPLTIFKNVSKVLPGHYLRISRSGILDESY
                     YELSPEKSNLTFQNATAKLAELLEESVKDQLVADVPLGSFLSGGLDSSIIALLAKKHK
                     PDLHTFSIGFRGNKFFDESDYSKLVSKHINSNHTVFEFAEKDLLEQVFASLDYLDEPF
                     ADSSAIAVNLLSRETRKHSTVALSGDGSDELFGGYNKHTAWVKSLEGASGWIKSFVPA
                     LSLLPGSRSGAFGNRVRQLRKFGAGVKLPTKERYWLWASLTDENGAKSLLSENSKSNL
                     SLENSEILKKTWLRFLDKENLNRHLLTDLHMVLPDDMLTKVDRMSMAHGLEVRVPFLD
                     KRVAEFAFSLPGHFKNDGNQSKIILKEAFKSQLPPGIIHRSKKGFEVPVLKWLRKDLK
                     KIISDDLLSESLLNQQGIFNSGEVKKLLRKLHSMNPGDSHARVWALVCFQWWWKKYYH
                     F"
     gene            complement(2530..3924)
                     /locus_tag="VGA21_06855"
     CDS             complement(2530..3924)
                     /locus_tag="VGA21_06855"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX9650259.1"
                     /translation="MTKKTINNLCFVICFLLISCTQPNHIHTILSVEVPSKGELYLMT
                     LDSLGFTVSQQLNFEGVNFKTVSLDLEFPAEVRLESTLIENQLRLRVTPGDSLAVAIV
                     LDSLPNHYSFRITGSDSTFQNYLLKKDLEFSRFVLESWQTQPENELVELDRMRSEWSR
                     QRNSASFESHLIRYDSLADWSRQLNFKLGKFMATPALDTSEIASLFHELSVYNPGLKK
                     VFDFNRLVFDLQYLFVTLSLLENDRLAEYYQGELLTTTIQTLSVPSLDPYILLWMKAI
                     MISEISDYGIDGDLGKEYQKYISSPDSPPVFIEALKQRAQIWLRLRAGNPAPDFEAIT
                     TAGDTVRLSDYFGKYLFLDVWASWCKPCIAEFPHSLVMMLDSRFKDFEFIFVNLDRNQ
                     ELWKQGMKQFNIPDGTHLFMPDDFDGEIFVQYNRTSIPRYILIDPQGKIINARAPRPS
                     QRDKIFETISDLAL"
     gene            complement(4125..5753)
                     /locus_tag="VGA21_06860"
     CDS             complement(4125..5753)
                     /locus_tag="VGA21_06860"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014021791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HEX9650260.1"
                     /translation="MKKILPFLIILLISCEQKYDTIITGGTIYDGTGGEPFIADIGIK
                     GDTIAFIGEIGFANAGNKIDASGMAVSPGFINMLSWANETLIMDGRGLGDLMQGVTME
                     VMGEGSSMGPLNDSMKAAIEARMVDYKYEIEWNTLGEYFDFMERKGISTNIASFVGNS
                     TIRQNVLGMNNVEANTEQREQMKNLIRQAMEEGAVGVSSSLEYVPSMYSDTYELIEFA
                     KIAAEYGGMYISHIRDEEEKLLEAVREIITIAIEANIRAEIYHLKASGASNWDKLDSA
                     IYLIEEARDSGISLTTDMYNYPASSTGLDIVLPNWAKEGGILKEIERLSDPELRRQIE
                     SEIDWNTPPDRILLVGFRNENLRHLTGKYLSEVATERDEAVSKVLCDLIVEDGSRIQV
                     VYFSMSEENIRKKIALPYMSFCSDAGSMAPEGVFLKQSTHPRAYGSFARLLSNYVREE
                     KIISLEEAVRKLTLLPALNLKLSRRGALKEGYYADIVIFDPETIDDLATFENPHQLAI
                     GVEHVWVNGIPVIANREHTGARPGKAIRGPGYRK"
     gene            complement(5880..7019)
                     /locus_tag="VGA21_06865"
     CDS             complement(5880..7019)
                     /locus_tag="VGA21_06865"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX9650261.1"
                     /translation="MDVVIIGGGIAGLSIARALEKNQLQTTLYESAPELKPVGAGIAL
                     AFNAMQACRSLGIYEEVSSKGLAIQKALITSYNGKIISEFDNSKFLPVFGDVSQAIHR
                     WDLQEALKNSLNTTEILLGKKVTGFIQTKKDVEIKFSDGSSTTTQYVIAADGVHSIFR
                     QKLIVHSEPVYSGYTCWRGIGEMPASYKWREYANESWGPGQRLGIVPLPVNRVYWFAV
                     KNAPEKDLAMIRSKKNDLLQFFRKWEPGMLKVIEETPEENIIFSDIIDIDPVDQFAFG
                     NILLTGDAAHATTPNMGQGGCQAIVDAAILDKCIGSTSTFEDAFRLFEQRRIDKTRRV
                     ILNSRRFGKIAQSENLFLIAIRNLLISLIPQKTSAKQIMWLMEEF"
     gene            7137..8045
                     /locus_tag="VGA21_06870"
     CDS             7137..8045
                     /locus_tag="VGA21_06870"
                     /inference="COORDINATES: protein motif:HMM:NF024245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEX9650262.1"
                     /translation="MYGKLLKWLRNYFGFSKTETYGVIVVHLAISVMLVLPIILEYYL
                     EKDFQPGEYNLHETKVSNAVEVKPEMLPADSLFAFDPNLATSVEFVKLGLKKFLAERI
                     INFRSKGGGFQSPDDLNKIFGIDSAWVEKVKPFVEIQQKTTVKSSAPPMKRNLVYSKK
                     DSAGFTKSSPVILDVNLADTTDLKRLRGIGSALSSRIIKFRDLLGGFVDMHQLTEVYG
                     LSPEVLELLSQNFLVDSGFLPEQIKINECTKEELQKHPYVSPKTAELMINYRLNHYPV
                     TPADLEKIPTISRDQLEKILPYLSFE"
     gene            8098..12105
                     /locus_tag="VGA21_06875"
     CDS             8098..12105
                     /locus_tag="VGA21_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013454696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HEX9650263.1"
                     /translation="MEKILQTYLRRLVNLSSNNRSLLLLRLASDQFIDIHDFNFLEKD
                     PSFAIIEKLIARNSEILLCKQVDSRDDQSNEVSRKLKKLKRIDDFIFEERGSRDLFVG
                     WPFIRGKFSDGTLVRAPLLFFPVSLEIEKNKWVLLLREDLTITFNKSFLMGYSYFNQV
                     KLDDELIERTFEDYDPDSTVFRTEIYQTFRDSPVDINFNPDNFTNNLVAFKNFIKANF
                     EKHEQNGELKLYPEAVLGIFPQAGSQLVPDYQEMLERGGDKDLEEFFLKRSIEHDKNQ
                     LHFAFLDQVREEDTYTPFKMDAFQENALKAVKKGNSLVVQGPPGTGKSQLICNLISDF
                     IARGKTVLVVSQKRAALDVVFDRMKSEGLADFLGLVHDFKNDRKKLYEKIALHIERIE
                     EYESRNNSLDIVHLERTFQQASRRIDQISEELEEFKFALFDTTECGISVKELYLTSDK
                     TQPGLNLKFEYRNFKFDDLSNFELEFQSYVELKQKFASNHPWNNRKSFAGYGLSELKR
                     MEEILVEIQERAIEINAEMNTMITHLFSYDELISLEEKKEDLRIMRSDLSNKEIFNHF
                     LFAVEFSDSDSELLWVSNMERIMMEFFVREGVEKSLAANKLGEFQEVLQKRLEARKNP
                     VKYLRWLMFSKDKYYLKRVMVANELRPTSKELKKLVKKIDNRLNLEHNLTKLKEKKWI
                     QEIPVSITKVDFQTWFEELKQGLRLKKQFTSVRLLQDYFSPIKETQEEFLQKISRLIE
                     LLEKVFVWKTSWETYLSIRQIQNIYSGELDIKATIKTLHKDFDNLCELDNLIQKLSND
                     EINVIEKALEKRNELGGSYDPWPLFSNSLRLAWIDHIESKYPILRSVSSIKFGKLEKE
                     LQSKVKEKIKVSNEILLVRVREKTYQNVLFNRLKNRISYRDLHHQATKKKRIWPLRKL
                     IDNYAEDLFNLIPCWLASPESVSAIFPMMPFFDLVVFDEASQTFTEQSIPAMYRGRQI
                     VIAGDNMQLRPNDLYKIRWDDESEDPSVEVDSLLELASQYLMSIMLQGHYRSKSLDLI
                     DFSNRNFYKGKLNLLPDKNVIERGEPSIKYIKVEGIWEDNRNDLEAREVARLVFDIMK
                     RNSDKEIGIVTFNARQQMHIMDVLDEESIVRKILLPQKLFIKNIENVQGDERDIIIFS
                     TGYGPNPNGKIILQFGSLNVEGGENRLNVAITRAREKIYVVSSLFPQQLKTGDSKNPG
                     PKLLKEYLEYSLEVSEGKFQTRPHEPAFKPEWYLKEKLKPIVLSLNSDLKVTQELPFA
                     DLTIKKNNNYLGLILTDDDLYNDSLSIKQMHVYKPFTLSSKHWKAKGIFSREFWNSQA
                     EVEDSIRIFVHQAKD"
     gene            12272..13582
                     /locus_tag="VGA21_06880"
     CDS             12272..13582
                     /locus_tag="VGA21_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004156168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEX9650264.1"
                     /translation="MELTKKKSEELMARRKKVVADGVGIFVNSTVAKAYNGTIVDTNG
                     EELIDFGGGIGVLNAGHCPPPVVKAIQKQAEQLIHSCFHVSTYEPYLELCEKLAQLFP
                     HGPQTKVMLTNTGAESVENAIKIARQATGRSAIIAYTEAFHGRSMMAMSLTSKIDYKK
                     GCGPFAPEVYRIPFPNYYHLGNGMGEDEFSNQELARLQDAFKSMVDPKNVAAIIIELA
                     QGEGGFNFAPKNYVKGLRKICDELGIVLIIDEVQSGFCRTGKWAAYLHYDITPDISTW
                     AKSMGSGMPIGAVVGKREVMDGAAPGTIGGTYLGNPVSCAAAIATISYMEEIDLNSKA
                     LQVAKLVSESMHRMKKSCNLIGDVRGLGAMQAMEFVKDDDPNKPDPETVSKFMNACLE
                     RGLLILNAGIYKNVIRILSPLTMPENQLKKGLAIMEEELEKLCK"
     gene            13590..14528
                     /locus_tag="VGA21_06885"
     CDS             13590..14528
                     /locus_tag="VGA21_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020532529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEX9650265.1"
                     /translation="MKPFSIAGVQMNVSASHSNVPAMKHQIDVMMAVYPWVQMVVFSE
                     LCPFGPLTYNALEFPNSIEGEFREIAKKYGIWLIPGSMFQKKDNLIFNTASVINPEGE
                     VIGRYSKMFPFYPYEAGVTGGNEFVIFDVPEVGRFGVSICYDMWFPETSRTLAVQGVE
                     VILHPTLTATIDRDIELSIAQATAATNQCFIFDINGLGDGGTGRSIICGPDGRVMHQA
                     STGPELIPIEIDIERVQRSRELGVLRLGQPLKSFRDRRVNFNIYEKNKKHPYLDSLGH
                     LIKPTRLKLLNNVLQTPVNPDEVTDFTDLDENNKNK"
     gene            14531..14947
                     /locus_tag="VGA21_06890"
     CDS             14531..14947
                     /locus_tag="VGA21_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002703380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX9650266.1"
                     /translation="MKSPAKDSKPKKKRKEKLNDYVAWFEIPAYEINRAVEFYNHVYD
                     LSLQIQDMAGYNMAIFPTNDGIGGAIVMGEGAIPSDKGSLVYLNGGKDLDVYLNRVPE
                     AGGRIILKKTLISKEHGYFALFIDSEGNKMAFHSKK"
     gene            14950..17736
                     /locus_tag="VGA21_06895"
     CDS             14950..17736
                     /locus_tag="VGA21_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020532530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX9650267.1"
                     /translation="MTVVKKLLANQYFNVTQFRIDLWNELKYAANKLKTTKKDKANEM
                     NSIESLFRSLSALEKYWAFPGMENFASLKQMFKRKEYTAFFNEISEIVRMLSSEAYRS
                     QPGMPEPKQKSKRIKKEEEANSITRNYFETLFVDNLSSKEEKNIKDKLSKLRSSDDPF
                     IYETLVANTFQDALIALLFNYNIQVCVIRYGIPFKSSNDISDIKTFISSITKLNFKNK
                     SENELGLLLCDWIKNLRPELDVYYVTDTAVGDIDDEALKNFRRIFYRKEDLQEIHLSI
                     NRGIKERFETPFFTALKEYSQKPTGVFHAMPISRGNSVFKSLWINDFGEFYGRNLFLA
                     ETSATTGGLDSLLKPTGPLKKAQQLAAKAFGAQQTFFVTNGTSTGNKIVVQALIEPGD
                     LVLIDRDCHKSHHYGLVLAGAYPVYLDSYPIEEYSMYGAVPIKALKAKLMRLKKAGRL
                     DKVKMCLLTNCTFDGLVYNVERVMEEVLAIKSDMIFLWDEAWFAFASFTPTYKQRTAM
                     YVADKLYKKYSSEKYKRDYATYKEGNGSTEKPMPDPRRVKVRVYATQSTHKTLSSLRQ
                     GSMIHIYDEEFKRKTEESFIESYMTHTSTSPNYQILASLDSGRRQVQFEGYEMVEKSI
                     EMAMILRYKINEHPKLRKYFDVLTVKDFIPAEFRESGIEEYYHTETGWTRMEEAWETD
                     EFVLDPTKINLFIGRTGVDGDTFKNKYLMDQFGIQINKTSRNSVLFMTNIGTTRSSVA
                     YLIGALLKIAEQLDEKFKSLNNDEALIEENKIYSLTKDVPPLPHFSHFHASFQAVPGV
                     PGGNIREAFFQAYNEKKCMYIKLEDCQKALDGGREIVSASFVIPYPPGFPILVPGQVL
                     SPDILEFLMKLDVKEIHGYRADLGLRIFTSSALNRHETPTAMMGAVLKESKADKSKKM
                     IQRSSKSTKGITKQ"
     gene            17774..19465
                     /locus_tag="VGA21_06900"
     CDS             17774..19465
                     /locus_tag="VGA21_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004156174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase"
                     /protein_id="HEX9650268.1"
                     /translation="MAKKSTQPKKTIRKSVKSKGKKSVKRPSPKKPKKITGKTPGKSR
                     QRKLKVTGTTRKVAAATKSKSKTQGKKSTGLSPKPLNGISDIRRFFFRNETPVYFISA
                     TNFNLLGADEWIKGFKFITYIECFDGLHPNVFSPKQQIEHDEFESIEDINNYLLEHKE
                     VIDYLESRKNSKKTGKVLFLMFDSKTEKLAKKLGLEICFPSAKMRSFMDNKVNTNRIA
                     EKAGVPPVPYVLAKVTSYVKLQQVSKKLGGDLVVQLPYGDSGHTTFFISNEDDWDKYA
                     DEIISEKEVKIMKRINCRGAAIEACVTRYGTIVAPLMTELVGFKELTPYRGGWCGNEI
                     FAEAFTPAIRRKARQYTRKFGDQLMKEGYKGYFELDFLIEESTDNIYLGELNPRVTGA
                     SSITNHAVFALADAPLFVFHLLEWMNYPYKLNPKTITNRWFKPENIDTWSQLVIKHTE
                     DSVEIITDAPRSGIWRMMQDGSIKFDRMDTHRRAVEKSTEAFFLRISRKGDYLYEGAD
                     MGILVMRGRLMTNEFKLTGRAINWIDAIRNLYKSEAPHDYFKTREKVGIAGFKMM"
     gene            19465..20457
                     /locus_tag="VGA21_06905"
     CDS             19465..20457
                     /locus_tag="VGA21_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020532533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family peptidase"
                     /protein_id="HEX9650269.1"
                     /translation="MHLCVNFVHEEKPGPKWLEIFDRTWPYYQKWFLSDNNKERPGYL
                     TCVENFQKFLPELYPVYNEIVETAGGGDLAARYLSMFNPPAYMSGCTQLAWKKENVAL
                     IRNYDYDSKFFEGTMLYSNWLKPVIAMVDCNWGVLDGINKDGLSISLTFGGRNITGFG
                     FGIPVVLRYLLETCSTVGACMIKLQKIPIHMSYNLTIVDASGDFATVFLSPDRPITTS
                     YSRVTANHQSEVEWMDYAILTSTRERENFLNNVLNDPAITEEQVVQKFFQKPVYQLKH
                     DKGLVTLYTALYHPQTRDLQIMWPDKSVFQSFDYFTERKVYVTLGRPVSGKLTL"
     gene            20869..21291
                     /locus_tag="VGA21_06910"
     CDS             20869..21291
                     /locus_tag="VGA21_06910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650270.1"
                     /translation="MEATAEITNKINKLYYSATVIYAEASEQEWIDNLLDGINAHKIE
                     LQTLNDLFEETLNDVYGEASKLSSKKDYIIYKEILQPLYDISEKLYHQASHHSWKGCF
                     NTEMNDFSILKEDLHELLIDLEKTIKGNKKSEQLLRIK"
     gene            21299..21700
                     /locus_tag="VGA21_06915"
     CDS             21299..21700
                     /locus_tag="VGA21_06915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650271.1"
                     /translation="MALLESQTYQKAYQKLVKKKTSYNSINQDLHEFFNEHKFDEIWQ
                     MRFFLRGNGGVRLLKLRLKNNKQKRGASGGYRLIVRCQQNTKEICLLYIYPKTGPLRQ
                     ENIEDTFEIKLLKKYNEQRKQGTLVLSNIPI"
     gene            complement(22147..22635)
                     /locus_tag="VGA21_06920"
     CDS             complement(22147..22635)
                     /locus_tag="VGA21_06920"
                     /inference="COORDINATES: protein motif:HMM:NF025474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility lipoprotein GldH"
                     /protein_id="HEX9650272.1"
                     /translation="MIRIHNIFIIFLLAGVLISCDSERIFETNENFSQKYWHKDSLKS
                     FSFHITDSNSTYSLYMNLRNTASYPFQNIYIQCQLKDSLNKVLEDQLLNFQLFHSQTG
                     EPFGSSGVGDIFDHQFKVFENYKFTASGSYSLHFAQMMRKDTLGNIMSVGARVEFFQP
                     EN"
     gene            complement(22619..>22913)
                     /locus_tag="VGA21_06925"
     CDS             complement(22619..>22913)
                     /locus_tag="VGA21_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013455461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Signal peptidase-like protein"
                     /protein_id="HEX9650273.1"
                     /translation="DIFRKLMWFGYEGENTWHPVDVSRVVEIQSLNKQGKKPATLLED
                     TDFAREEKVRAISRDLERLDKKFADKGGRKKRKNKKRKNRNPNNRQRPNDKNS"
BASE COUNT         7318 a         4295 c         4696 g         6604 t
ORIGIN      
        1 tcgcttaggt tatgaggtag aactggcaaa tttgactgaa agtgggcgaa gttatatttc
       61 aaaaggattt ttggatgaag caaggaaaat tgccattgaa aatgtttcga aagcatctga
      121 gtttgttaat cccgaaaatc ctttaattgg aattgagccg tctgcaattc tttctttcag
      181 agatgaatac attgacttgg tggaagtgga aaaacgagaa aaagccacta acctttcaga
      241 aaattgttta cttctggaag aatttctctc cagggaaatt gacgcgggta aaattacctc
      301 aaagcaattt acggataagg ccgaaacagt aaaagtgcat gctcattgtc atcaaaaatc
      361 gctatcatct ctcactcatt taaaaaaagc tctttcagtg ccaaaaaatt atcaggttca
      421 ttttttacga accggatgct gtggcatggc gggttcattt ggttatgaga aagaacatta
      481 tgacctctct atgaaaattg gaaatcttgt actgtttccg gcattaaaga aattaacttc
      541 agaaattgtt gcggctcccg gaacaagctg ccggcaccag atcaaagatg ggacgggaat
      601 gaaagctttg catcccgcgg aaattctgct aaaggcttta cggtaatgtg tgggatcacc
      661 ggtatttatg cttttaacca aattggccgg tttcaaatga tcaatgcggc tagggcgacg
      721 gaagcgttgc acacccgcgg gcctgatttc caaagaacca tcaataatga atacgcgtct
      781 tttggtcacc ggcggctttc tataatagat acttctcctg cgggtaatca accgatagaa
      841 gatgagcgca actggctggt ctttaatgga gaaatctata attaccggca attgcgttct
      901 gaacttgagt ctgccggcga atcattttac agccatagcg attcagaagt gctgtttcgt
      961 cttttaaaaa gtcgtggttc tgaagggata gaaaaattgc agggagattt tgcctttgca
     1021 ttttatgatg cagcagaaga ttcggtactt atcgcacggg atcgttttgg agtcaaacct
     1081 ctttattatt ttaaagatga agataaattt atttttgcat ctgagattaa atccatactt
     1141 agttatggac ttgatttgga attggatcac gatgcattgt taatttatct tcagcttaat
     1201 tatatccccg cacccttaac catttttaag aacgtttcca aggttcttcc cggccattat
     1261 ctcagaattt cccgttcagg catcctggat gaatcttact atgaattatc tcccgaaaag
     1321 tcaaacttaa cttttcaaaa cgctactgca aagcttgctg aacttttgga agaatctgtt
     1381 aaagaccaac ttgttgcgga tgttcccctg ggttctttct taagcggtgg tttggatagc
     1441 tccatcatcg cgttgctggc taaaaagcat aaacccgatc tgcatacctt ttccattgga
     1501 ttcaggggga ataaattctt tgatgaatcg gattattcaa agctggtatc aaaacatatt
     1561 aattcgaatc acacagtctt tgagtttgct gagaaggatt tgctggaaca agtatttgca
     1621 agtctcgatt atctcgatga acccttcgcg gactcttccg ctatagcagt taatctgcta
     1681 tccagggaaa ccaggaaaca tagtactgta gccttgtctg gtgatggaag tgatgaattg
     1741 ttcgggggtt ataacaaaca tacagcctgg gtgaaaagtc tggaaggagc gagcggatgg
     1801 ataaaatctt tcgtaccggc actcagtctt ttaccgggtt ctcgttccgg tgcttttgga
     1861 aatagagtaa gacaattgcg aaaatttgga gccggtgtaa aattaccaac taaggaacgc
     1921 tattggttat gggcaagcct gacagatgag aatggggcaa agtcgttgct tagtgaaaat
     1981 tccaagtcaa acctttcatt ggaaaattct gaaatcttga aaaaaacctg gctgaggttc
     2041 cttgataagg agaatcttaa ccggcattta ttgacagatt tacacatggt tttacctgac
     2101 gacatgctaa ctaaggtaga tcgaatgtca atggctcacg gtcttgaagt gagggtgcct
     2161 ttccttgaca agcgtgtggc tgaatttgca ttttcattgc caggacattt caagaatgac
     2221 ggcaatcaaa gcaaaataat tttgaaagaa gccttcaaaa gccaactgcc gcctggaatt
     2281 attcaccggt ctaaaaaagg atttgaggtt ccggtgctaa aatggcttag aaaagatctg
     2341 aagaaaatta tttctgatga tctactttca gaatcactgc tgaatcaaca gggaattttt
     2401 aattccgggg aggtgaaaaa acttctgcgg aaattacatt caatgaatcc cggtgattct
     2461 catgccaggg tgtgggcgct ggtgtgcttc caatggtggt ggaagaaata ctaccatttc
     2521 tgaatctctt caaagagcca aatcggaaat tgtttcaaaa atcttgtctc tttgtgatgg
     2581 gcgcggcgct ctggcattta tgattttgcc ctgaggatct attaatatat atcgcggaat
     2641 ggaggttcgg ttatattgca caaagatttc tccatcaaaa tcatcaggca tgaataaatg
     2701 tgtaccgtca gggatattaa attgcttcat tccctgtttc cacaattcct gatttcgatc
     2761 aagattgaca aaaatgaatt caaaatcttt gaatcgtgaa tcaagcatca taacaagact
     2821 gtgaggaaac tcggcaatgc agggtttaca ccagctggcc catacatcca ggaataaata
     2881 tttaccaaag taatccgata gcctaactgt atcacctgct gtagtaatag cttcaaaatc
     2941 aggagcagga ttgccggccc taagtcttaa ccatatctga gccctttgct ttaaggcttc
     3001 tataaagaca ggcggtgagt ccggactgga aatgtatttt tggtactctt ttcctaaatc
     3061 accatcaata ccgtaatcgc taatctcact tatcattata gctttcatcc atagcagaat
     3121 ataaggatcc aaagacggaa cggaaagtgt ttgaattgtt gtagttagca attctccctg
     3181 ataatattct gccaaccggt cgttctcaag aaggcttagg gttacaaaca agtattgcaa
     3241 gtcaaaaacc agcctgttaa aatcaaaaac tttctttaat cccggattat aaacagaaag
     3301 ctcatgaaat aaagatgcaa tttcagaagt atcaagcgcg ggtgtggcca tgaacttacc
     3361 gagtttaaaa tttagttgcc tggaccaatc agcaagactg tcatatctta ttaaatggga
     3421 ttcgaaagat gcagagtttc tttggcgact ccactctgat ctcatcctgt cgagttcaac
     3481 taattcattt tcgggttgag tttgccatga ttccaaaaca aaccggctaa attccaaatc
     3541 ctttttcaac aaataatttt gaaacgtgga atcactacct gttatacgaa agctataatg
     3601 gttgggcaat gaatccagta ctattgcaac tgccaaagaa tctcccggag ttacccgtaa
     3661 cctgagctga ttctcaatca atgtagattc aagtcttacc tcggctggaa attccaaatc
     3721 cagcgacacc gttttaaaat tcactccctc aaaatttaat tgttggctaa cagtaaaacc
     3781 cagactatca agggtcatta aatacaattc cccttttgag ggcacttcaa cagaaagtat
     3841 ggtatggata tgattcggct gagtacagga aataagcaaa aagcaaatca cgaagcaaag
     3901 attattaata gttttcttag tcatactatg gaatttcata acgaagtacg atgaaaatca
     3961 taaaaacctg aggtataatg atcccaaatt cacaataact acctgacttt aattcaaaca
     4021 tcaggattca aggcaaaatt gcatcaaatg attctgagga aggcaaaaac tgcaaagaaa
     4081 tcgaatttgg ggtaaaaagt ttgtgccaaa agttataaaa tcccttattt cctatagcct
     4141 gggcctctta ttgcctttcc cggccttgcg ccggtatgtt ctctattggc gattaccgga
     4201 atcccattta cccaaacatg ctctactcca atagccagct ggtgtggatt ctcaaatgtt
     4261 gccaggtcat ctattgtttc cggatcaaaa ataacgatat cggcatagta gccttctttt
     4321 agtgcccctc gccgggaaag tttcagattt agcgcgggta ataaggttaa ttttcttact
     4381 gcttcttcca atgaaataat cttctcttcc cgcacatagt tggatagcag ccgtgcaaaa
     4441 gatccgtaag cacgggggtg agtggattgc ttcagaaaaa ctccttccgg agccattgat
     4501 ccggcatcag aacaaaaact catataaggc agggcaatct ttttgcggat attttcttca
     4561 gacatactga aatagacgac ctggatccgg ctaccgtctt caacaataag atcgcaaaga
     4621 actttagata cagcctcatc tctttccgtg gcaacttcag agagatactt tcctgttaaa
     4681 tgccggagat tttcgttccg gaaaccaacc aatagaattc gatccggtgg tgtgttccag
     4741 tcaatttcag attctatttg gcgacgcaac tcaggatcac ttaatcgctc aatctctttt
     4801 aaaattcctc cttcctttgc ccaattcgga agcactatgt cgagtccggt tgaactggcg
     4861 gggtagttat acatatcagt tgtcaatgaa atgcctgaat ccctggcttc ctcgatcaaa
     4921 taaatagcgc tatcaagttt atcccagttg gaggcaccgg atgctttcag gtggtatatt
     4981 tccgcccgaa tgtttgcttc tatggcaata gtaattattt cacgaacagc ctccagcagt
     5041 ttttcttcct catcacgaat atgagaaatg tacatgccgc cgtattcagc cgcaattttt
     5101 gcaaactcaa tcaactcata agtatcactg tacatgctgg ggacgtattc aagggaagaa
     5161 gaaacaccaa cagcaccttc ttccattgcc tgcctgatta aatttttcat ttgttcacgc
     5221 tgttcggtat tagcctcaac attattcatc cccagcacat tttgccttat tgtagaatta
     5281 cccacaaaac ttgctatatt cgtggaaata cccttacgtt ccataaaatc aaaatactct
     5341 cccagtgtat tccattcaat ttcatattta taatcaacca ttctcgcttc gattgcagct
     5401 ttcatggagt cattcagagg tcccattgat gacccttcac ccattacttc catcgtaacg
     5461 ccttgcatca gatcgccaag ccctcttccg tccatgatca aagtctcgtt ggcccaactc
     5521 agcatattaa tgaagccggg agagacggcc atccctgaag catcaatttt gttacctgca
     5581 ttcgcgaagc caatctcacc tataaatgcg attgtatctc ctttaatgcc aatgtcggca
     5641 atgaacggtt cgcctcccgt tccgtcataa atggtgccgc cggtaataat tgtatcatac
     5701 ttctgttcac aagatatcag gaggattatc aggaagggaa gaattttttt catgtatata
     5761 aagtttggga ctatgaaagt aagaagaaaa gatgagactg gataacgcgg attcatggat
     5821 ttgtatttcg ctgaaaaatt tttccttatt aattcaaact cattaattca cactcagact
     5881 taaaactctt ccatcaacca cataatttgc ttagcgctgg ttttctgcgg tattaggctg
     5941 atgagcaaat tccggattgc aataagaaac agattctcgc tctgggctat ttttccgaat
     6001 ctccgcgaat taagaattac tctcctcgtt ttatcaattc gtctttgttc aaataatctg
     6061 aatgcatcct caaaagtaga agtactccca atgcatttat caagaattgc ggcatccaca
     6121 atggcctgac atccaccctg gcccatgtta ggtgtggtag catgagcagc atcaccggtt
     6181 agcaagatgt tcccaaatgc aaattgatct acgggatcaa tgtctatgat gtcgctaaaa
     6241 attatattct cttccggagt ctcttcaata actttaagca tgccgggttc ccacttcctg
     6301 aagaactgaa gcaaatcatt tttctttgac cgtatcattg ccaaatcttt ttcaggggca
     6361 ttcttaacag caaaccaata cacccggtta acaggcaggg gaactattcc caacctttgc
     6421 cccggacccc agctttcgtt tgcatattct ctccatttgt atgaagccgg catttctccg
     6481 attccacgcc agcaagtata tccggaatac accggttctg aatgtactat cagcttttga
     6541 cggaaaatgg aatgaacgcc gtctgcggca attacgtact gagtagtggt agaggaaccg
     6601 tcagaaaact ttatctcaac atctttttta gtttgaataa aaccggtaac tttttttccc
     6661 agcaaaattt cagttgtgtt gagggaattc ttcaacgcct cctgcaaatc ccaacggtga
     6721 attgcctgtg aaacatcccc aaaaacagga aggaacttgc tgttatcgaa ttcactaata
     6781 atctttccat tgtaagaagt aatcaatgct ttttggatgg ctaaaccttt gcttgaaact
     6841 tcctcataaa taccaagaga ccggcaggct tgcatggcat tgaaagcaag agcaatgcct
     6901 gcaccaactg gtttcagttc cggagcagat tcatataatg tcgtttgtaa ttgatttttt
     6961 tccagcgccc tggcaatcga aagcccggct attcctcctc caataattac gacatccata
     7021 taatcttgag attattaatg actaagttcc taattttttg caattaagat ttgcataagt
     7081 tctctaatcg ttaataagat taagtataaa aaatcaataa atatttatat ttacgaatgt
     7141 atggtaaatt gttaaaatgg ttgcgcaatt attttggatt cagtaaaact gaaacctatg
     7201 gagtaattgt ggtccatctg gccatatcag tgatgcttgt tcttccaatt attttggaat
     7261 actatcttga aaaggatttc cagcccggtg aatataattt acatgagacc aaagtatcaa
     7321 atgcggttga agtgaaaccg gaaatgttac cagcagattc attgtttgca tttgatccaa
     7381 atcttgccac ttcagttgaa tttgtaaaat tggggctgaa aaaattcctg gcggaaagaa
     7441 ttatcaattt cagatccaaa ggaggtgggt ttcagtcgcc ggatgacctt aataaaatat
     7501 ttggaattga ttccgcctgg gtagaaaaag taaagccttt tgttgaaatt caacaaaaaa
     7561 ccacggttaa atcgagtgca cctccaatga aacgtaacct ggtttattca aagaaagatt
     7621 cagctggatt cacgaaatca agtccagtaa ttttggacgt aaatcttgcc gataccaccg
     7681 acctcaaaag gttgcgtgga attggttcag ccctttcatc gaggataata aaattcagag
     7741 acctcctggg tggttttgtt gatatgcacc agttaacgga ggtttatggt ctttctcccg
     7801 aagttcttga actattaagt cagaattttt tagttgactc cggatttctc ccggagcaaa
     7861 taaagataaa tgaatgcacg aaagaagagt tgcagaagca tccttatgta tcacccaaaa
     7921 ccgctgagct tatgatcaat taccggttaa atcactatcc tgtaacgccc gcggatttgg
     7981 agaaaattcc cactatttcc agggatcaac tggaaaagat tctcccatat cttagtttcg
     8041 aataggtatt gatttaatcc aagcgttttt tggtttatct tgatacatct ttcaggaatg
     8101 gagaaaatcc ttcagacata tcttcgcagg ctggtaaatc tatccagcaa taatcgctct
     8161 ttacttttac tcaggctcgc atcagaccag tttattgaca ttcacgattt caattttctt
     8221 gaaaaagatc cctcgttcgc aataattgaa aaactaatag ccagaaatag tgaaattttg
     8281 ttatgcaaac aagttgatag ccgtgatgat caatcaaacg aagtaagcag gaaattaaaa
     8341 aaattaaaac gcattgatga ttttattttt gaagagagag gctcacggga tttgtttgta
     8401 ggctggccat ttataagagg aaaattttct gatggaactc tggttcgggc accattgctt
     8461 tttttcccgg tttccctcga gatagaaaaa aacaagtggg tgcttttatt acgcgaagat
     8521 cttactataa cattcaataa atcttttttg atgggttatt catattttaa ccaggtaaag
     8581 cttgatgacg aattaattga aagaactttt gaagattatg atccggacag tacagtcttc
     8641 cgcacggaga tatatcaaac atttcgggac agcccggtcg atataaattt taatcccgat
     8701 aatttcacaa acaatctggt tgcatttaag aatttcatta aagctaattt tgaaaaacac
     8761 gaacaaaacg gagagctgaa attatatcct gaagctgtct tgggtatttt cccacaggca
     8821 ggttctcaac ttgttcccga ctatcaggaa atgttggaaa ggggtggtga taaagatttg
     8881 gaggaatttt tcctaaaacg aagtattgag cacgataaaa atcaattgca tttcgcattt
     8941 cttgatcaag tgcgggaaga agacacctat actcccttta aaatggatgc gtttcaggaa
     9001 aatgcactta aagcggttaa gaaaggaaat tcattggtgg tgcaaggacc gccaggtaca
     9061 ggaaagtctc agctaatctg caatttaatt tccgatttta ttgcccgggg aaaaaccgtt
     9121 cttgtcgtaa gccaaaaaag agcagcactg gatgtcgtat tcgatcggat gaagtccgag
     9181 ggtttggccg acttcctggg attggtgcac gatttcaaaa atgatcgtaa aaaattatat
     9241 gagaaaatag ctttacatat tgaacgtatt gaggaatatg agtccagaaa caattcatta
     9301 gatatagtac atctggagag aacatttcag caggcaagcc ggaggataga tcagatttcg
     9361 gaagaactgg aggagtttaa gtttgcacta tttgatacca ctgaatgtgg catttctgtt
     9421 aaggaattat acctcacctc agacaaaacg cagcccggcc tcaatctgaa gtttgagtat
     9481 agaaatttca agtttgacga cctgtctaat ttcgaacttg aatttcagag ctacgtagag
     9541 ttaaagcaaa agttcgcgag caatcatccc tggaacaatc gtaagtcttt tgcaggatat
     9601 ggtctctcgg aattgaaaag gatggaagaa atcctggttg aaatccagga aagagcgata
     9661 gaaataaatg ctgaaatgaa caccatgata acccatttat tctcttatga tgaactaatt
     9721 agccttgaag aaaaaaaaga agatttaaga atcatgcgaa gtgatttaag taacaaggaa
     9781 attttcaatc attttctatt cgctgtagag ttttctgatt ctgactctga gcttttatgg
     9841 gtttccaata tggaacgaat aatgatggaa ttttttgtca gggagggagt agaaaagtca
     9901 ctggctgcta ataagttggg tgaattccag gaggttcttc aaaaaagact tgaagccaga
     9961 aagaatcctg tcaaatatct aagatggctg atgttcagca aagacaaata ttatttgaaa
    10021 agagtaatgg ttgccaatga attaagacca acttcaaaag aacttaaaaa actggttaag
    10081 aaaatcgaca atcggttaaa ccttgaacac aatctcacaa agctaaagga aaagaaatgg
    10141 atacaagaaa ttccggtcag cattactaaa gtggattttc aaacctggtt tgaagagtta
    10201 aaacaaggac taagattaaa aaaacaattt acctcagtcc ggctattgca ggattacttt
    10261 tccccaatca aggagactca ggaagaattt ctgcaaaaga taagccggtt aattgaatta
    10321 cttgaaaaag tatttgtatg gaaaacttcc tgggaaactt acctgtctat caggcaaata
    10381 caaaatattt attccggtga gttagatatt aaggcaacta taaaaacgct tcataaagat
    10441 tttgataatc tctgtgaact ggataatctg attcagaagt tgagcaatga tgaaataaat
    10501 gtaattgaaa aagcgcttga aaaaagaaat gagttaggtg gtagttatga cccctggccg
    10561 ctttttagca atagtctcag acttgcctgg attgatcaca tagaatcaaa atatccaatc
    10621 ttgcgatcgg tgtcttcaat aaaattcgga aaacttgaga aagaactaca gtccaaagtg
    10681 aaagagaaaa taaaagtgag caatgaaatt ttattggtgc gggttaggga gaaaacatat
    10741 caaaatgttc tgtttaaccg gctgaaaaac aggatttctt accgggatct ccatcatcag
    10801 gcaactaaga agaagcggat ctggccgtta agaaagttaa tagataatta tgccgaagat
    10861 cttttcaacc tgataccatg ctggctggct tctccggaat cggtttctgc aatatttccc
    10921 atgatgccat tttttgatct tgtcgttttt gatgaagctt cgcaaacgtt taccgaacaa
    10981 agcatccctg ccatgtaccg gggccgtcag attgtaattg ccggcgacaa tatgcaattg
    11041 cggccaaatg atctttataa gatacggtgg gatgatgaat ctgaagatcc atctgtcgaa
    11101 gtggattcgc ttcttgaatt ggcgagccaa tatttaatga gcatcatgct gcagggtcat
    11161 tacaggagta aatcgcttga cctgatagat ttcagcaacc gcaattttta taaaggaaag
    11221 ctaaacttgt tgccggataa gaatgtgatt gaacgaggcg agccttctat taaatacata
    11281 aaggtagaag gaatctggga agacaataga aacgatctgg aagcaagaga agttgccagg
    11341 cttgtatttg atataatgaa aagaaattca gacaaagaaa tcggaatagt tacatttaac
    11401 gcgcgccagc aaatgcatat catggatgtg ctggatgaag aatcaatagt tcgaaaaata
    11461 ctccttcctc aaaaactgtt cataaagaat atagaaaacg ttcagggaga tgagcgggat
    11521 atcataattt tttccacagg atatggacca aaccccaatg gaaaaattat tttacaattt
    11581 ggaagtctta atgtagaagg tggtgaaaat agactgaatg tggcaatcac aagagcaaga
    11641 gaaaagattt atgtggtatc cagccttttt ccacaacagt tgaaaactgg ggattcaaag
    11701 aatccagggc cgaagttatt aaaggaatat cttgaatatt cgttggaggt aagtgaagga
    11761 aaatttcaaa cccgccctca tgagccggcc tttaagccgg aatggtacct taaagaaaaa
    11821 ttgaaaccca tagtgttgtc attaaactca gatttgaaag tgacccagga actgcccttt
    11881 gctgatttaa ctattaagaa aaataataat tacctgggtt tgatacttac cgatgatgac
    11941 ctctacaacg atagcctctc catcaaacaa atgcatgttt acaaaccctt tactctttct
    12001 tcaaaacatt ggaaagctaa aggtatcttc agcagagaat tttggaatag ccaggctgaa
    12061 gtggaagact ccatcagaat ttttgttcac caggctaagg attagccagc ctttttgatt
    12121 ttgattccag cctaataatc gctattttat aaaaatgcaa aattctatat ctcaaaatta
    12181 agattttagt gtttcccgat ggagaaaatt aattaattaa accaaaccaa tcttttaacc
    12241 tgttaacttt gaaagctaaa ttttaatacg aatggaactg acaaagaaaa agagcgaaga
    12301 actaatggct cgtcgaaaaa aggtagtagc cgatggagtt ggcatctttg tgaattcaac
    12361 ggtggccaaa gcatataacg gaaccattgt agataccaat ggagaggaat tgattgactt
    12421 tggcggggga attggcgtat tgaatgcggg tcattgtcca cctccggttg taaaggcaat
    12481 ccaaaaacag gcggaacaat taatccacag ctgttttcat gtctctactt atgaacctta
    12541 tcttgaattg tgcgaaaaat tagcgcaact cttcccgcat ggaccacaaa cgaaggtaat
    12601 gcttaccaat actggagcgg aatctgtaga aaatgcgatt aagattgcgc gtcaggccac
    12661 gggcagatcc gcaatcattg cttataccga agcttttcac gggagatcaa tgatggccat
    12721 gagtctcact tcaaaaattg attataaaaa gggttgcgga ccatttgccc ctgaggttta
    12781 ccggattcca tttcccaact attatcatct tggaaatgga atgggtgaag atgaattttc
    12841 gaatcaggag ctggccaggt tgcaggatgc ctttaagagc atggttgatc caaaaaatgt
    12901 cgcggcaata ataatagaac ttgcacaagg ggagggcgga tttaatttcg cgccgaagaa
    12961 ttacgttaag gggttaagaa aaatttgtga tgaattgggg attgttttga ttatcgatga
    13021 agtgcagtca ggattttgca gaaccggaaa atgggccgcg tatcttcatt atgatattac
    13081 ccccgacatt tccacctggg caaaatcaat gggttccgga atgcctatag gtgcagtggt
    13141 aggtaaaaga gaagtgatgg atggtgccgc tcccggaaca atcggtggta cctacctggg
    13201 caacccggta agttgtgctg ccgcgatcgc cactattagt tatatggaag aaattgattt
    13261 gaatagcaaa gctttgcagg tggcaaaact ggtttcagaa agtatgcacc ggatgaaaaa
    13321 aagctgcaat ctcattggtg acgttcgcgg attgggcgcc atgcaggcaa tggaatttgt
    13381 aaaagacgat gatccgaata agccagaccc ggagacagtt tcaaaattca tgaacgcttg
    13441 cctggagaga ggtctgctga ttttgaatgc aggaatttat aaaaatgtaa tccgcatact
    13501 gagtccgcta acgatgccag agaatcaatt aaagaaaggc ctggctatta tggaagagga
    13561 gttggaaaaa ctttgtaaat aaattcagga tgaaaccatt ttctattgca ggagtgcaaa
    13621 tgaacgtatc tgccagtcat tctaatgtac cggccatgaa acatcagatt gatgtaatga
    13681 tggcggtata tccatgggtg caaatggtag tatttagtga actatgcccc tttggaccat
    13741 taacgtataa tgccctggaa tttccaaatt ccattgaagg tgaattccgg gaaatagcta
    13801 agaaatacgg tatctggctc atcccaggtt ctatgtttca gaaaaaagat aatttaattt
    13861 tcaatacagc atcagtaatt aatcccgaag gagaagtaat tggcaggtac agcaaaatgt
    13921 ttccatttta tccttatgaa gccggtgtaa ccggtggcaa tgaatttgtt atttttgatg
    13981 tacctgaggt agggagattt ggtgttagta tttgttatga catgtggttt ccggaaacca
    14041 gcagaacact tgcggttcag ggagttgaag taattttgca ccctacgctc actgcaacta
    14101 tcgaccggga catcgaactt tcgattgcgc aagccacagc agctaccaat caatgtttca
    14161 tatttgatat taacggtctt ggagatggag gaaccggaag aagtataatt tgtggtccgg
    14221 atggcagagt aatgcatcag gcgagtaccg gccctgaact tatccccatt gaaattgata
    14281 ttgaacgcgt tcagcgaagc agggaattgg gtgtactcag acttgggcag ccgttgaaaa
    14341 gtttccgtga caggagggtg aatttcaata tttatgaaaa aaataaaaag catccttacc
    14401 tggattcact tgggcacctg ataaagccaa ccaggctaaa attattgaac aatgttttac
    14461 aaactcctgt aaatcccgat gaagtaactg attttaccga ccttgatgaa aataataaaa
    14521 ataaataatt atgaagtcac ctgctaaaga cagtaaacca aaaaagaaac gtaaggaaaa
    14581 gttgaatgac tacgtggcct ggtttgaaat tccggcatac gaaattaacc gtgctgttga
    14641 attctataat catgtctacg atcttagcct tcaaatccag gatatggcag gatataatat
    14701 ggcaatcttt ccaactaatg acgggattgg aggtgcaatc gtaatgggtg agggagcaat
    14761 ccccagcgac aaaggctcac tggtctatct caatggcggg aaagatctgg atgtttacct
    14821 gaaccgcgtt ccggaagcag ggggaagaat tattttaaag aaaactttaa ttagcaaaga
    14881 acatggctac tttgctttat ttatagattc agaaggcaac aaaatggctt tccactcaaa
    14941 aaaataatta tgactgtagt caagaaactt ctggccaacc aatattttaa tgtgacacaa
    15001 tttcgcattg atctttggaa tgaactcaag tatgctgcca acaaactcaa aacaacaaag
    15061 aaagataagg caaatgaaat gaattcaatt gaatctcttt tcaggtcatt atcggcgctg
    15121 gagaaatact gggcttttcc gggaatggaa aattttgctt cactcaaaca aatgtttaaa
    15181 agaaaagagt acactgcttt ttttaatgaa atttctgaga ttgtcagaat gctcagtagt
    15241 gaagcatacc ggagccagcc aggcatgccg gagcctaaac aaaaatcaaa gcggataaaa
    15301 aaggaagaag aagcaaattc aataacccga aattattttg aaactctttt tgtagataac
    15361 ctttcttcca aagaagaaaa aaatataaaa gataaactct caaagcttcg cagtagcgat
    15421 gatcccttta tatatgaaac ccttgttgcc aacacttttc aggatgcttt aatcgcactg
    15481 ttatttaatt ataacattca ggtttgtgta atccggtatg gtatcccatt caaatcaagc
    15541 aatgatattt cggatataaa aacctttatt tcttcaatca caaaattgaa tttcaaaaat
    15601 aaaagtgaaa acgaattggg gttattactt tgcgattgga taaaaaattt gcggcccgag
    15661 ttagatgttt attatgtaac cgatactgcc gttggagata tagatgatga agctttgaaa
    15721 aacttccggc ggatttttta tagaaaggaa gatttgcagg aaatacatct tagtattaac
    15781 cgggggataa aggaaagatt tgaaacgcca ttcttcacag ctttgaagga gtacagccaa
    15841 aaacctaccg gcgtttttca tgcaatgcct atttcaaggg gcaactctgt gtttaaatct
    15901 ctttggataa atgattttgg tgaattctac gggagaaatc tttttctggc cgagacttcc
    15961 gccaccaccg gcgggctgga ctctttgctg aaacccaccg gtccgcttaa gaaagctcag
    16021 cagctggcag caaaagcttt cggtgcccag caaactttct ttgttactaa cggaacatcg
    16081 acagggaata aaatcgtggt gcaggctttg atagagccgg gcgacctggt gctgatagat
    16141 agggactgcc ataaatctca tcattacgga cttgttttag ccggtgctta tccggtttac
    16201 ctggattcct atcccataga agaatactca atgtacggtg cggttcctat caaagctctc
    16261 aaagcaaagt taatgcgcct caaaaaggcg ggccggcttg acaaggtgaa aatgtgtctc
    16321 ctgacaaatt gcaccttcga tggcctggtg tacaatgtgg aaagagttat ggaagaagtg
    16381 ctcgccataa aatccgacat gatctttctt tgggatgagg cctggtttgc ttttgcatca
    16441 tttaccccaa cctataagca gcgtactgcg atgtatgtgg cagataaatt atataagaaa
    16501 tattcttctg aaaaatataa acgtgattac gcgacttaca aagagggaaa tggcagtact
    16561 gagaaaccca tgccggatcc tcgccgggtt aaagtgagag tttatgctac gcaaagtacc
    16621 cacaaaaccc tttcctcctt aaggcaaggt tcaatgattc atatttatga tgaagaattt
    16681 aaacgaaaga ctgaagagtc gttcatcgaa tcatatatga cgcacacctc tacttcacca
    16741 aactatcaaa tactggcttc cctggattca ggacgaaggc aagtacagtt tgaaggttac
    16801 gaaatggtag agaaaagtat tgaaatggcc atgattctgc ggtacaaaat taatgaacat
    16861 ccaaagctga gaaagtattt tgatgtgctt acggtaaaag attttattcc tgcggaattt
    16921 agagagtcag gcattgaaga atattatcac acagaaactg gatggacacg aatggaggaa
    16981 gcctgggaaa cagatgagtt tgtgctggat ccaactaaaa ttaatttgtt catagggaga
    17041 acaggagttg acggtgatac tttcaagaat aaatatttga tggatcaatt cggtattcaa
    17101 atcaataaga cttcccgcaa ttcagtcttg tttatgacta acattggaac gacgcgaagc
    17161 tcggtagctt atctgattgg cgctttatta aagattgcgg aacaacttga tgagaaattc
    17221 aaatcattga ataatgatga agctttgatt gaggagaata agatttactc tttgacaaaa
    17281 gatgtaccgc cacttcctca cttcagtcat tttcatgcct ctttccaggc tgttcccgga
    17341 gttccgggag gtaacatcag ggaagcattt ttccaggcat acaatgaaaa gaaatgcatg
    17401 tacataaaac ttgaagattg ccagaaagcc ctggatgggg gaagagaaat cgtctccgcg
    17461 tctttcgtta ttccttaccc gcctgggttt cctatcctgg tgcccggtca ggtattaagt
    17521 ccggatattt tggaattcct gatgaaactg gatgtgaaag aaatccatgg ataccgggct
    17581 gatcttggct tacgaatctt tacctcctcg gcacttaatc gccatgaaac gcccaccgca
    17641 atgatgggcg ctgttctgaa agaatcgaag gccgataaat ccaaaaaaat gattcagcga
    17701 agctcaaaat ctacgaaggg aataacgaaa caatgaggat tgccgctaac atgtaataat
    17761 tgataaaaat tctatggcaa aaaaatctac gcaaccaaaa aaaacaatca gaaaatctgt
    17821 aaaatcgaaa ggcaaaaaat cagttaaaag accatctcca aaaaaaccaa agaaaatcac
    17881 tggcaaaact cccgggaaat ccaggcaaag aaaattgaaa gtaaccggca caacccgaaa
    17941 ggtagcagcc gcaactaaat caaaatcaaa aactcaggga aaaaaatcta caggattgtc
    18001 tcccaaacct ttgaatggaa tttccgatat ccgcagattc tttttcagaa atgaaacacc
    18061 tgtttatttt atttcagcta caaattttaa cctgttaggt gccgacgaat ggatcaaggg
    18121 gtttaagttc attacttaca ttgaatgttt cgatggcctt catcctaacg tgtttagtcc
    18181 gaagcaacaa attgaacatg atgaatttga aagtattgaa gatatcaaca actatttact
    18241 tgagcataaa gaagtaatag attatctgga atccaggaag aattcaaaaa agaccggtaa
    18301 agtgctcttc ttaatgtttg actccaagac tgagaaactt gcaaagaaac ttggattgga
    18361 gatatgcttt ccttcagcta aaatgaggag ctttatggat aataaagtaa acacaaaccg
    18421 gatcgccgag aaagcgggcg tgccacctgt tccctatgtg ctggctaaag ttaccagcta
    18481 cgttaaactt caacaagttt caaaaaaact aggcggggat ttggtagtac aattaccata
    18541 tggtgattca ggtcacacta ctttctttat ttcaaatgaa gatgattggg ataagtacgc
    18601 tgatgaaatt ataagcgaga aggaagtaaa aatcatgaag cgtattaatt gccgtggagc
    18661 ggctattgag gcgtgtgtaa ccaggtatgg aacaatcgtt gctccgttaa tgacagagtt
    18721 ggttggtttc aaagaattaa ctccgtaccg gggaggctgg tgcgggaatg aaatattcgc
    18781 agaagccttt acgcctgcca ttaggagaaa agccaggcaa tacacgcgga aatttggaga
    18841 ccaattgatg aaggagggtt acaaaggata ttttgaactg gatttcttaa tcgaagaaag
    18901 tactgataat atttatttgg gtgaactgaa tccgagagtt acgggggcaa gctccattac
    18961 gaatcacgca gtttttgcac tggctgatgc cccattgttt gttttccact tactggaatg
    19021 gatgaattat ccttataaat tgaatcctaa aactattaca aacaggtggt ttaaacctga
    19081 aaacatagat acctggagcc aactggtaat caagcatacc gaagattcgg tagaaattat
    19141 aactgacgct ccccgttccg gaatctggcg gatgatgcaa gatggaagta taaaatttga
    19201 caggatggat acacaccggc gggctgtgga aaaatcaacc gaggctttct tcctgcgaat
    19261 ttcccggaaa ggtgattatt tatacgaagg tgccgacatg ggtatcctgg taatgcgagg
    19321 cagattaatg actaatgagt ttaaattaac tgggcgcgca attaactgga tagatgccat
    19381 tcgaaatcta tataagagcg aagctccgca tgactatttc aagacaaggg aaaaagtggg
    19441 tatagctgga tttaaaatga tgtaatgcac ctgtgcgtaa attttgttca tgaagaaaaa
    19501 cccggcccta aatggctgga aatttttgat cgcacctggc cttattatca gaagtggttc
    19561 ttatctgata acaacaaaga acgcccggga tacctgacct gtgtcgaaaa ttttcagaaa
    19621 tttttgcccg agctttaccc ggtttataat gaaattgtgg aaaccgcggg tggaggagat
    19681 cttgccgcaa gatacctgag catgtttaat ccccctgcct acatgtcagg ttgtacgcag
    19741 ttggcctgga aaaaagaaaa tgtggctctg atcagaaatt acgattacga ttcaaaattc
    19801 tttgagggta ccatgctcta ttcaaattgg ctaaagcctg taattgccat ggtcgattgc
    19861 aattggggag tgttggatgg tataaacaaa gacggtcttt ccatttcatt aacttttgga
    19921 ggccgcaata ttaccggatt tggatttggg attccggtcg tgctaagata cctgcttgaa
    19981 acttgcagca cagtgggagc gtgcatgatc aagctgcaaa aaattcccat tcatatgtct
    20041 tataatctga ctatcgttga tgcgtcggga gattttgcga ccgtatttct cagccccgac
    20101 cggccaataa caacatccta ttcccgggta actgccaatc atcagtctga agtggagtgg
    20161 atggactatg caatccttac ttcaaccagg gaaagggaaa actttttgaa taatgtgctt
    20221 aatgatcctg caatcactga agaacaggta gtgcaaaagt tcttccagaa accggtctac
    20281 cagctaaagc atgacaaggg attggtgact ttatacaccg ccctgtacca tccgcaaacc
    20341 cgcgatttgc aaatcatgtg gccggataaa tcagtatttc aatcctttga ttattttaca
    20401 gagagaaaag tgtacgtcac gctggggaga ccggtttcgg gaaaacttac tctttaattt
    20461 tcaggctgaa aattataatt ggattacact tcagatctag ccctaattga aaggatataa
    20521 ttttcaaagt ccttttgctc tttctctgcc aatttcctta gttttttaaa aggtttccgg
    20581 cgtctatgca taagggttca ttatcttatc tttcatataa gtaaaggcaa tcattgtaaa
    20641 atatgcaaat aattgcttta ctaattaacc ccaaaaagct catttattac cttttatcac
    20701 ctaaaatgta tcattaagtg aatgtttttc gttaacttgt aaataagact aagaaaaagg
    20761 taacttttgc agtcatttta ttaaatgcat tttcttgcat gcttttatcc tggatatttt
    20821 tttgtaattt atcatcccct tataaagttg aggtgaaaat ttaaaataat ggaagcaact
    20881 gcagaaatca caaacaaaat caataagctt tattatagtg caactgttat ctatgcagaa
    20941 gcatcagaac aagaatggat tgataattta ttagatggaa ttaatgcgca taaaatagag
    21001 cttcaaaccc taaatgattt gtttgaggaa actttgaatg atgtttatgg tgaggcatca
    21061 aaattatcta gcaaaaaaga ttatattatt tacaaagaaa tccttcagcc attatatgat
    21121 atatctgaaa aactgtatca tcaggcatct catcattctt ggaagggttg tttcaataca
    21181 gaaatgaatg atttttcaat tttgaaagaa gatctacatg aattattaat agatctggaa
    21241 aaaacaatta aggggaacaa gaaatccgaa caattattaa ggataaaata aatgaaattt
    21301 ggcccttcta gaatcccaaa cttatcaaaa ggcatatcaa aaattagtta aaaagaaaac
    21361 ctcatataac tccattaatc aggatttgca tgaatttttt aatgaacata aatttgatga
    21421 gatatggcaa atgaggtttt ttttaagagg gaatggtggt gttcgattgc tcaaattaag
    21481 actgaaaaat aacaagcaaa aaaggggtgc gagcggtggc tatagattaa ttgttcgttg
    21541 tcaacaaaat accaaagaaa tctgcttgtt atatatttat cctaagaccg ggcctctaag
    21601 acaagagaat attgaagata cttttgaaat aaaattgttg aaaaaatata acgaacaaag
    21661 aaaacaagga actctagtcc tttcaaatat acctatatga tcacatacca ggacagtgcc
    21721 aaattatgca taaactccgc gactaagtaa ctgcataaag gatttaaatt tgcaaacggt
    21781 tttcgagtat agctaaaaat gggtttacaa taagtggcca attccgaatt gtcataatct
    21841 ttctttttga taatctcctt taaggaagta ttgttttcga ctaactaaga tttctcaaat
    21901 tatacattac ctcttaacat actttaatta tgaaaacctg aatactggga gaatcttgct
    21961 aggcctaatt cgttttttcg cttcttccaa taatttatat attttgggat actatcatgg
    22021 tggcggtctt aaagaaagaa gaaccttacg ggaaaagtat cattaattca attccaattt
    22081 ccgagattac tataaggatt tataagtaga attctttatt tcccgacaat cgggaaaact
    22141 taccctttaa ttttcaggct gaaaaaattc cacccgtgct ccaaccgaca taatattgcc
    22201 aagagtatct ttacgcatca tttgcgcgaa atgtaaacta taacttcctg atgcagtgaa
    22261 cttataattt tcaaaaactt tgaactgatg atcgaaaatg tctccaactc cgcttgaacc
    22321 gaaaggttca cctgtttgtg aatgaaacaa ctgaaaattc agaagctgat cctccaacac
    22381 tttattcaga gagtccttta actggcattg aatataaata ttttgaaagg gatatgaagc
    22441 agtatttctg aggttcatat aaaggctata tgtggaattg ctgtctgtta tgtggaatga
    22501 aaaagattta agtgaatctt tatgccagta tttttgggag aaattttcat tggtttcaaa
    22561 aatcctttca gaatcgcatg atattaacac gcctgctaat aggaagatga taaaaatatt
    22621 atgaattctt atcattaggt ctttgcctgt tattagggtt ccgattcttg cgttttttgt
    22681 tcttccgttt tttgcgtccg cctttatctg caaatttttt atccagacgt tccaaatcac
    22741 ggctaatcgc tctgactttt tcttcacggg caaagtcggt atcttcaagc agtgtagcgg
    22801 gttttttgcc ttgtttattc aggctttgaa tttctacaac acgggataca tccaccggat
    22861 gccaggtgtt ttcgccctca tatccaaacc acataagctt tctaaagatg tct
//
LOCUS       DASWHX010000104         9256 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_464138, whole genome shotgun sequence.
ACCESSION   DASWHX010000104 DASWHX010000000
VERSION     DASWHX010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 9256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9256
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_464138"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(659..946)
                     /locus_tag="VGA21_06930"
     CDS             complement(659..946)
                     /locus_tag="VGA21_06930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650274.1"
                     /translation="MTIKFLSPLTISFFILITASIIQVITDRSGGWGWVVASTFTITG
                     SILLAAHFILIFFLKDRPPLIWWIETAIVILLIIYAWKDGFPFESLLKPWK"
     gene            complement(968..2311)
                     /locus_tag="VGA21_06935"
     CDS             complement(968..2311)
                     /locus_tag="VGA21_06935"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX9650275.1"
                     /translation="MSHITPKRQKDGFWKYYLTNSISSIRYALLLSIILYAAFSVLDI
                     YMAPRSLKEIWIIRFIIIIPSFSMAFYLTFRKAFTFYNEVILFGVAMIGICGILAMIA
                     IVDRIEPAFRFYYCGLILCIIVSHSILRLRHSIALILSAVTICGYNLVILIFHPDFLN
                     EGELAILVNNNFFLLGSGVIGAWASSTSEKMFRKDYENQQLLLNEKSELILTKEKLQE
                     ANAVKNRLISIISHDIRGPISSLAGISKMFEDRQLSESELKYFMSKMGETLDGTTIML
                     NNMLSWSVMQMNNTRLVKSYILLRPVIDEILLTLKAQACLKKNQLQNLVDAEIKIHSE
                     PTLLRLVLSNLVTNAIKFTENGLIKVIASEKDNCTEISVTDTGLGMADEVSEKLFKWG
                     SRFTREGTKKEKGSGIGLIICKDLVEKQGGRIFCESKYGHGSKFTFALPKTFEFV"
     gene            2462..2728
                     /locus_tag="VGA21_06940"
     CDS             2462..2728
                     /locus_tag="VGA21_06940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650276.1"
                     /translation="MINQEHIGRLGKNLKQILDSEIALGNRVSETSKGWPNSRTVIVF
                     LEKPFLADYPIQGKGIIFREVNDPHYWKSEYYDRINDHVLACKF"
     gene            2822..3676
                     /locus_tag="VGA21_06945"
     CDS             2822..3676
                     /locus_tag="VGA21_06945"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HEX9650277.1"
                     /translation="MRAIWKGHIRFSLVTIPVRIYGAIDAQQTISFNQINKKTNNPIR
                     YEKKDKGTGETVKSEDIVKGYQYEPGQFVIMNPEDFEKVKLKSTRVIEIEGFVDAKEV
                     HPALFDTPYFAGPDGEVAAKTYSLLCKTLQQSGKLGIGRVVLRDRENVLLLAPEKNGI
                     LLYKLRYPEELRNITDVPDITDDQADKEQLKLAKMLVDNMTTKFETLELKNRYNDALK
                     DIIDKKIEGEEIVVYEEEEAPVVDIMTALKESISQAKEQKKPMKKATGKAKEEKARSS
                     TKKKKKTA"
     gene            3683..4285
                     /locus_tag="VGA21_06950"
     CDS             3683..4285
                     /locus_tag="VGA21_06950"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9650278.1"
                     /translation="MGRVIKFPVNASPRFKLKPVLSKKSKKGAESQLDIFKSPDSKLV
                     QMSGSLSPFEEALIYDENGNLKARAMYEKAISMNDHSPDAWCNLGIIEYLEGNKSKAV
                     SCFTHSLKEDPRHFEAHYNLANVYLDIENLELARLHYEVAAEIDPEHADVFYNLAVVY
                     AMEKEFEKAIDSLENHLKLVSQKDSNDARGFLLSLKASIE"
     gene            4357..5223
                     /locus_tag="VGA21_06955"
     CDS             4357..5223
                     /locus_tag="VGA21_06955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650279.1"
                     /translation="MVNLISKLNHLPSEHTANLESICDEIIETGKADIIILFGYFAFS
                     HTKIPVNSSYEILVITDHFETREQLSNELAVRFQKNSPPIHGVIETITLFNISVEEGL
                     FYFSDILREGIILYNNEEFELAKPKDLSPLKRRKIAEEDYHQGMAQAKEAWKNYQTES
                     SKNNHKGCAFHLNQVAEFCYMAVEMVFSHNYYDEHNIKAQRDRIRAFDKRIHKAFPLD
                     NKSQIEAFEYLSQAYVGAQYMSDSDFPISQKQIDYWARESRKLIVLTDSICKGKIQRL
                     KDVRRKLRESRN"
     gene            5287..5682
                     /locus_tag="VGA21_06960"
     CDS             5287..5682
                     /locus_tag="VGA21_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008616446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HEX9650280.1"
                     /translation="MSYYFNTIVKNTDFEGAVVRVTEELKKEGFGVLTEIDVKETLKK
                     KIDVDFKKYKILGACNPPFAYEALRAEDKIGVFLPCNVIVEENEDGQVEVSAVDPVAS
                     MSSVKNESLGTLANQVRKKLQKVIKNLSA"
     gene            5682..6350
                     /locus_tag="VGA21_06965"
     CDS             5682..6350
                     /locus_tag="VGA21_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018343252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX9650281.1"
                     /translation="MAIRAIITGATGMIGEGVMHTCLNHDKVESVIAITRKPSGYNHE
                     KLTEIIHSDFGNIESIKNQLRDSNAVFFCAGVSSVGKNEAEYKKATYDLTLNFAHTLL
                     EISPEINFCYISGAGTDSTEKGRTMWARVKGKTENDLLALGFDSAYMFRPGYIQPIKG
                     LKYTLPMYRYISFLYPLLKSFMGKYVVTLEELALAMINSNLSGYEKKILECSDIVNLA
                     RREN"
     gene            6356..6679
                     /locus_tag="VGA21_06970"
     CDS             6356..6679
                     /locus_tag="VGA21_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015029740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEX9650282.1"
                     /translation="MVSVNTFRKMATSFKEVEELPHFDKTSFRVRKKIFSTLEVKRKR
                     ACLKFTDMQQADFIAYNSDIVYPVPGKWGKMGWTYFELSKVPSSIVKDALRISYLDIA
                     PKSLR"
     gene            6702..7328
                     /locus_tag="VGA21_06975"
     CDS             6702..7328
                     /locus_tag="VGA21_06975"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX9650283.1"
                     /translation="MISEPVMKNEIIKLITPNLFLRILNNSDAENLRNLLARNKNFMI
                     PWIPWASEEPQSLEKKIQKIQEWKIEFLANQKYTYGIFTPDQKAMIGLIFLFTRQGKG
                     KLEIGYIIDFEESGKGFATEASYAMTKLGFEHLKIENMVIHCNSKNIASAKIPEKLGY
                     QIVQKKMESNIEMLIWSFPKEKFQIIKNFEPVLLLNDKINRKRKSLRS"
     gene            complement(7517..8188)
                     /locus_tag="VGA21_06980"
     CDS             complement(7517..8188)
                     /locus_tag="VGA21_06980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650284.1"
                     /translation="MKITGSDKIGFGVLSLFLIGAIIIGFSNEHYFENNFAVEDGIVE
                     WTTTLLLFASSVYLVSRIILLRKAKPKFRMLCTLLLSALFFFGAGEEISWGQRILGME
                     TSEFFEKNNAQKETNFHNLVVGETKINKLIFGQLLTVILVIYLLIIPYFYTKWSWLNK
                     LISLFGVPLPRLHHSIAFIAVTALIMIIPSSKRWEVYELAFAMIFLLIFLNPKNEEIY
                     QKSIL"
     gene            8280..8666
                     /locus_tag="VGA21_06985"
     CDS             8280..8666
                     /locus_tag="VGA21_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011584486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX9650285.1"
                     /translation="MSTPLKGLRTTIYYVSDLERAKEWYARAFGQEPYFDEPFYVGFE
                     IGGYELGLHPGEKKKAETESVISYWGVDDIQKEFKRLMDLGGKSHQEPMNVGDNIMVA
                     SVRDPWNNIVGIIYNPNFRVKSSQAG"
     gene            complement(8663..>9256)
                     /locus_tag="VGA21_06990"
     CDS             complement(8663..>9256)
                     /locus_tag="VGA21_06990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650286.1"
                     /translation="NVYNANLRILENEQETKLLVKDIEIKRREKAIIILIIASGLLTA
                     FTIFVIILYRYVSGLNRSLKREKEKLKKKRDELSIYARNLKSLVESKTESIYHKNMQL
                     AGYAFLNAHKMRSPITTLLGLVQLTEGNKQMEDEILLREIKKNVIRLDRISKHINQIL
                     AEEMDLINDDKMKKYFANFKESIENYNENDPDTLKEN"
BASE COUNT         3043 a         1636 c         1806 g         2771 t
ORIGIN      
        1 ccactcgtaa aatcttccca atttccggta atggtgtaac attttggagc gaattagaag
       61 gtatgaacca aagcaggaag tgatttaaga gcaactgggt agctggaaga aaacatcaga
      121 cttcaacaca gccgggatat ctgactttct ttcgttaaag ccagaaatta cctccaatca
      181 gtaagaatgt tgcggacaaa tctgattgct gtgcggaaag gtttatttcc agggcttcaa
      241 gagtgattca aagggaaatc cgtctttcca cgcgtagatt attaaaagaa ttacaatgcc
      301 gatttcaatc caccatatta atggaggctt atcttttagg aagatctcaa cttatcgcac
      361 aattagttca aattgtgtga tttttttcac attcatgtaa ctaattgtgt gaattatggt
      421 ttttcagaag gtattcctta aaaaatatgg aacacagaaa cggattaatt aatattccat
      481 tgttcctgat tgatcggaca aaagatttta tcgaacaggt gatttaaggg agactgagcg
      541 gctgaagaaa acttcagact tcaacacagc cgggatatct gcctttcttc cttaaaccaa
      601 aaattacgtt caatcagtaa gaatgttgcg ggcaaatctg attgctgtgc agaaaggttt
      661 atttccaggg cttcaagagc gattcaaagg gaaatccgtc tttccacgcg tagattatta
      721 atagaattac aatggcagtt tcaatccacc atattaatgg aggtctatct tttagaaaga
      781 aaattaaaat gaagtgtgca gccagaagaa ttgatcctgt aattgtgaat gtagaggcaa
      841 caacccaccc ccacccacca gatctatccg taattacctg gattattgat gctgtgataa
      901 ggatgaaaaa tgaaattgtg agtggagaaa gaaattttat ggtcatttca atttaaacgg
      961 ttaggcatca aacgaactcg aaagtttttg gcagcgcgaa tgtaaatttc gatccatgcc
     1021 cgtatttgct ttcacagaaa attctcccac cctgcttttc aacaagatcc ttacaaatga
     1081 ttaatccgat tcccgaaccc ttttcctttt ttgttccctc ccgggtaaac cggcttcccc
     1141 acttgaacag tttttcactt acctcatcgg ccatacctaa tcctgtatca gttacggaaa
     1201 tttcagtgca attatctttc tcgctggcta tcacttttat tagcccattc tctgtaaatt
     1261 taattgcgtt tgtaaccaga ttacttaata caagtctaag caaggtgggc tccgaatgaa
     1321 tcttaatttc cgcatcaacc aggttttgca attgattctt tttcaggcaa gcctgagctt
     1381 tcaatgttaa aagtatttca tcaatcaccg gccgcaacag aatgtaagat tttactaatc
     1441 tggtattgtt catctgcata actgaccagg aaagcatgtt atttagcatt atggttgttc
     1501 catccagggt ttctcccatt ttactcatga aatattttaa ctcgctttca cttaactgtc
     1561 tatcttcaaa cattttggaa atccctgcga gacttgaaat gggcccacga atatcatgtg
     1621 agattatgga aattaaacgg ttctttaccg cgttggcttc ctgcaatttt tctttggtca
     1681 gaattaattc acttttttca tttaacagca gttgctggtt ttcataatcc tttctgaaca
     1741 ttttttcact tgtgcttgag gcccacgccc caattactcc tgagccaaga agaaaaaaat
     1801 tgttattaac taatattgcc agttctcctt cattgagaaa atcaggatga aaaataagaa
     1861 ttaccaggtt gtatccgcaa atagttaccg cgcttaatat cagggcaatg ctgtgccgaa
     1921 gtctaagaat gctgtgtgaa acaattatac aaaggattag tccgcaataa taaaacctga
     1981 aagctggctc gatcctatca acaatcgcaa tcattgccag aattccgcaa atgccaatca
     2041 tggcgacacc aaacaagatt acttcattat agaatgtaaa ggcttttctg aatgtcagat
     2101 aaaatgccat tgaaaagctg gggattatta ttatgaaccg aatgatccag atttccttta
     2161 atgagcgcgg ggccatataa atatccagaa cactgaaagc cgcatacagg ataattgata
     2221 agagcaatgc ataacgtatt gaagaaatag aattggtcag ataatatttc cagaatccgt
     2281 ctttctgtct tttaggggtt atatgtgaca aaattcgact tataattttt ttatgtttga
     2341 atgtacctaa aggaactaaa ttggagaata ccttcaaatt taccaatagt atattagata
     2401 ttattattta aagatattat tgtattaact atgtaatatt gccggtaatt tagtatttat
     2461 tatgatcaat caggaacata ttggaaggct tgggaaaaac ctgaaacaaa tcctggattc
     2521 agagatcgct ctgggaaaca gggtcagtga aacaagcaaa ggctggccta attccagaac
     2581 agtgattgtt tttttggaga agccattcct tgctgattac cctattcaag gtaagggaat
     2641 aattttccgg gaagtcaatg atcctcatta ctggaaatct gaatactatg accgaatcaa
     2701 tgatcatgta ctggcttgta aattttaagc aggaattttt tgtttcgatg tttcttttat
     2761 acccacctga tcttaggatt tccacccata tttatatact ttaggagaaa aatacattcc
     2821 catgagagct atttggaaag gacatattcg attctccctg gtaaccattc cggtcagaat
     2881 ttatggagca atcgacgcgc agcaaactat cagttttaat cagatcaata agaaaaccaa
     2941 taatccgatc cggtatgaga agaaagataa agggaccgga gaaaccgtga aatctgaaga
     3001 tattgtcaaa gggtatcagt atgaacccgg gcagtttgtt attatgaatc cggaagattt
     3061 tgagaaagta aaactgaagt cgacccgggt tatagagatt gaaggttttg tggatgcgaa
     3121 agaagtgcat cctgccttat ttgatacgcc ttattttgcc ggaccggatg gagaggtagc
     3181 cgcaaaaacc tattcattgt tatgcaaaac cttgcagcag agcggtaaac ttggaattgg
     3241 cagagtagtt ctacgcgatc gcgaaaacgt tttgctcctg gctcctgaaa agaatggtat
     3301 tcttctatat aaactgcgtt atccggaaga gttgagaaat attaccgatg ttcctgatat
     3361 taccgatgac caggctgaca aagaacaatt aaagcttgcc aaaatgcttg tggataatat
     3421 gacaaccaaa tttgaaacac ttgaattaaa gaaccgatat aacgatgctt tgaaagatat
     3481 tatcgacaag aagattgagg gtgaagagat cgtggtttat gaagaagagg aagctccggt
     3541 ggtggatata atgacggcat taaaggaaag catcagtcag gcgaaagagc aaaagaaacc
     3601 gatgaaaaaa gcgacgggca aagccaaaga agaaaaagcc aggtcatcaa ccaaaaagaa
     3661 aaagaaaacc gcataaaacc gaatgggtcg agtaatcaaa tttcctgtaa atgcttcgcc
     3721 ccggtttaag ctcaaaccgg ttctctcaaa gaaatcaaaa aaaggagcgg agagccagtt
     3781 agatattttt aagtcacctg acagcaagct cgtgcaaatg tcaggtagtc tttcaccttt
     3841 cgaagaggct ttgatttacg atgaaaatgg aaatttgaaa gcccgggcta tgtacgaaaa
     3901 agccatctcc atgaatgatc actcaccgga tgcctggtgt aacctgggaa taatcgaata
     3961 cctggaagga aataaaagta aggcggtaag ttgcttcact cattcattaa aagaagatcc
     4021 ccgtcatttt gaagcacatt acaacctggc gaacgtttac ctggatattg aaaacctgga
     4081 gcttgctcgg ttgcattatg aagttgcagc agaaatagat cctgagcatg ccgatgtttt
     4141 ttataacctt gccgttgtat atgccatgga gaaagaattt gaaaaagcta ttgattcatt
     4201 ggagaatcat ttaaaattag tttcacagaa agactccaat gatgccaggg gatttttgct
     4261 tagcttgaaa gccagtatcg aataggttgc cccaggcaca tagtattatt attttccctt
     4321 gaagaattcc caactccggt taaattactg ccgtacatgg taaatttaat atcaaagctt
     4381 aatcatctgc cctccgaaca tactgccaat cttgaatcaa tctgtgatga aatcattgag
     4441 accggcaaag ctgatatcat tattcttttt ggatatttcg ctttttccca caccaagatt
     4501 ccggtaaatt cctcctatga aattctggtt attaccgatc attttgaaac ccgggagcaa
     4561 ttatctaatg aactggcggt taggtttcag aaaaattctc ccccgattca tggtgtaatt
     4621 gaaacgatca cactttttaa tattagtgtt gaagagggat tattttattt ttccgatatc
     4681 cttcgggaag ggattatcct atacaataat gaggagttcg agcttgccaa acctaaggat
     4741 ctttctccct taaagagaag aaaaatagca gaagaagatt atcatcaggg gatggcacag
     4801 gccaaagaag cctggaaaaa ttatcagaca gaatcaagta aaaataacca caaaggatgt
     4861 gcatttcatc ttaaccaggt ggctgagttt tgttatatgg ctgtggagat ggtcttctcg
     4921 cataattact atgatgagca taatatcaaa gcgcaacgtg acaggatacg tgcatttgat
     4981 aaaagaattc ataaggcttt tccactggat aacaaatctc aaattgaagc ctttgaatat
     5041 ttaagccagg catacgtggg tgctcaatac atgtctgata gtgatttccc gatttcacaa
     5101 aagcaaattg actattgggc acgtgaatcc aggaaattaa ttgtattaac cgattcgatt
     5161 tgcaaaggta aaatccaaag attaaaggat gtgagaagaa aactaaggga aagccggaac
     5221 tgattagaat ccttctccgt tgacggcttt taacgtatct tagaccagat acaaaaacaa
     5281 aaactcatga gctattattt taatacaatt gtaaaaaaca ccgattttga aggggcggtt
     5341 gtaagggtaa ctgaagaact taaaaaggaa ggttttggtg tcttaactga aattgatgtt
     5401 aaggaaaccc taaagaagaa aattgatgtg gatttcaaaa aatacaaaat acttggtgcc
     5461 tgtaacccgc ccttcgctta cgaggcattg cgggcagaag ataagattgg agtatttctc
     5521 ccttgtaacg tgatagttga agaaaatgaa gacgggcaag ttgaagtttc agctgtggat
     5581 cctgttgcat ccatgtcttc ggttaagaat gaaagccttg gtacactggc caaccaggta
     5641 cggaagaagt tgcaaaaagt aattaaaaac ctttccgctt aatggcgata agagcaatta
     5701 ttacgggtgc aaccggaatg attggtgaag gagtaatgca tacgtgcctt aaccatgata
     5761 aggtagagtc agtgatcgct attaccagaa aaccatcagg gtataatcac gaaaagctca
     5821 ctgaaattat tcattcagat tttggcaaca ttgaatctat caaaaatcag ctaagagaca
     5881 gcaatgcagt gtttttctgt gccggcgttt cttctgttgg aaagaatgaa gctgagtata
     5941 agaaggctac ctatgattta accttgaatt ttgcacatac ccttctggaa atttcaccgg
     6001 aaataaattt ctgttacatt tcaggtgcag gtacagatag tacagaaaag ggcagaacca
     6061 tgtgggcaag agtaaaaggc aagacggaaa atgatttgtt ggccctggga tttgattcag
     6121 cttacatgtt tagacctggg tatattcaac ccataaaagg gttaaagtac acgctgccca
     6181 tgtacagata tatttctttt ctttacccat tattgaaatc ttttatggga aaatatgtgg
     6241 taacactgga agaattagct ttagcaatga ttaacagtaa cctgtccgga tatgaaaaga
     6301 agatcctgga atgctctgac attgtaaatc ttgcgagaag agaaaattaa gaagcatggt
     6361 ttcagttaat acttttagaa aaatggctac ttctttcaaa gaagtagaag aattgcctca
     6421 ttttgataag acttcctttc gcgtcaggaa gaaaattttc agtacgctgg aggtaaaaag
     6481 aaaaagagct tgcttaaagt ttaccgatat gcaacaagca gacttcattg cctataattc
     6541 agatattgtt tacccagttc cgggcaaatg gggtaaaatg ggctggacct acttcgaatt
     6601 gagcaaggtt ccatcttcta tagtgaaaga tgcattaaga atttcctatc tggatattgc
     6661 tcctaaaagt ttaagataaa atttaatgag aaataaatta tatgatttct gaacccgtga
     6721 tgaaaaatga gattattaag ctaataacgc ctaatctttt tttgcggatt ttgaataatt
     6781 cagatgctga aaatttgaga aacctgttag ctagaaataa aaatttcatg attccctgga
     6841 ttccctgggc cagtgaagag ccgcagtcgc tggaaaagaa aatacaaaaa attcaggaat
     6901 ggaagattga atttcttgcc aatcaaaaat atacttatgg catttttact ccggatcaaa
     6961 aagctatgat tggattaatt ttcctcttta cccggcaggg taaaggcaag cttgaaatag
     7021 gatacattat cgactttgaa gaatcgggca aaggatttgc caccgaggcc agctatgcta
     7081 tgacaaaatt aggttttgaa catcttaaaa ttgaaaatat ggttattcat tgcaattcca
     7141 aaaatattgc cagcgcaaaa attcctgaaa aattaggata ccagattgtg cagaagaaaa
     7201 tggaaagcaa tattgaaatg ttgatttggt ctttcccaaa agaaaaattc cagattatta
     7261 aaaactttga gccggtatta ctgctaaatg ataaaataaa tcggaaacga aaaagtttaa
     7321 gatcctgaat ggaagaattt tactgcctgg gttgacgggc aattgtctta gtaattttaa
     7381 ttagcctctg caagccctgc agaatttttg cttgttttta ttcctcatga ttaatgttga
     7441 agtttagagt caaagacaca atcactttga aatgattgtt gatggagaat ttcgccatca
     7501 gtccagagta actaaatcag aggatggatt tttgatagat ctcttcattt tttggattca
     7561 aaaagataag gaggaatatc atagcaaaag caagttcata aacttcccac cttttgcttg
     7621 atggaattat cattattaag gctgtcactg caatgaatgc aatggaatga tgcagcctgg
     7681 gcaggggaac cccgaacaag ctaatcaatt tattaagcca gctccatttg gtgtagaaat
     7741 aaggtattat cagtaagtaa ataaccaaaa taaccgtaag aagctggccg aatatcagtt
     7801 tatttatttt tgtctctcca acaaccaaat tatgaaagtt ggtttctttc tgagcattat
     7861 tcttctcaaa aaactcagag gtttccattc caagaatcct ttgcccccaa cttatttctt
     7921 ctccggctcc aaagaaaaac agggcgctta ataacaacgt gcaaagcatc cgaaatttgg
     7981 gtttggcctt cctgagtaat atgattcttg ataccaggta aacactgctc gcgaataaca
     8041 ataaagttgt tgtccattct acaataccat cttccaccgc gaagttattt tcaaaataat
     8101 gttcatttga aaatccaata attatcgctc caataaggaa taaggacaaa actccaaaac
     8161 ctatcttatc tgaaccggta attttcataa ttaaatcaaa agtcgcaatt aatgatgaat
     8221 tgcattacaa aagttttcat tttaatataa atttgaaagc aatttaatta ttgaatataa
     8281 tgagtactcc attaaaaggt ttaagaacta ctatttatta tgtctctgat ctggagagag
     8341 cgaaagaatg gtacgccagg gcatttggac aagagccata ctttgacgag ccattttatg
     8401 tgggatttga aattggcggc tatgaattgg ggcttcatcc tggtgaaaaa aagaaggctg
     8461 aaactgaatc tgttatatcg tattggggtg tcgacgacat tcaaaaagaa tttaagaggt
     8521 tgatggatct tggcgggaaa tcacaccagg agccaatgaa tgtgggggat aatatcatgg
     8581 ttgcatccgt tagagatccc tggaataata ttgtcggcat tatttataat ccaaacttta
     8641 gagtgaaatc ttcccaagca ggctaatttt cttttaaagt atcaggatca ttttcattat
     8701 aattttcgat actttcctta aaattggcaa aatacttttt cattttatca tcatttatca
     8761 aatccatttc ttctgccaaa atctgattta tgtgtttaga aatcctatcc agccgaataa
     8821 catttttctt aatttcacgt aacaaaattt catcttccat ttgcttatta ccctcagtta
     8881 gttgaaccag accgagtaag gtggtaattg gtgaacgcat tttatgggcg ttgagaaaag
     8941 catagccggc aagttgcatg ttcttgtgat agatactttc tgtcttggat tccacaagac
     9001 tcttcaggtt tcgggcataa attgaaagtt catcccgttt ctttttcaat ttctcttttt
     9061 ctcttttcag ggatctattt aatccggata catatcgata tagaataata acaaaaatag
     9121 tgaaagcagt aagtaagcca cttgcaatta ttaaaatgat tattgctttt tcacgacgtt
     9181 ttatttctat atccttaacc aataatttcg tttcctgttc attttctaat atccggagat
     9241 ttgcattata tacatt
//
LOCUS       DASWHX010000105        13138 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_1578572, whole genome shotgun sequence.
ACCESSION   DASWHX010000105 DASWHX010000000
VERSION     DASWHX010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 13138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13138
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1578572"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(119..661)
                     /gene="hslV"
                     /locus_tag="VGA21_06995"
     CDS             complement(119..661)
                     /gene="hslV"
                     /locus_tag="VGA21_06995"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020600773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HEX9650287.1"
                     /translation="MKKTEIHATTVIAVRHMDSVAIGADGQATMGNYVAKSNVRKIRK
                     LQDGKVIAGFAGSTADSFTLLERFDEKLSSYGGNMKRAAIELAKDWRTDRFLRRLEAM
                     LIVANKEEILILSGTGDVIEPDHDIAAIGSGSMYAQSAAMALKKFSGNLSAEEIVKES
                     LTIAADICIYTNHNFVIEKL"
     gene            complement(664..2286)
                     /locus_tag="VGA21_07000"
     CDS             complement(664..2286)
                     /locus_tag="VGA21_07000"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009583497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex dihydrolipoamide
                     acetyltransferase"
                     /protein_id="HEX9650288.1"
                     /translation="MAEVIRMPKMSDTMEEGVIAKWLKKVGDEVKAGDILAEVETDKA
                     TMELEAYEDGTLLHIGIKDGEAAPVNGIIAVIGKKGESFDDLLKDEGGNASSNGKDDK
                     KLGMDGKETSKKNEEERDTSDIKASVITMPKMSDTMTEGTIANWLKKVGDTVKSGDII
                     AEVETDKATMELEAYEDGTLLYIGVKAGESVEVDGVIAVIGEKGADYQSLIKAHKQKA
                     TISVSATVKTQAPPFAESAPVEQIQETNPEKQGLANGRILASPLARKMAKDKGYDINL
                     ITGTGDNGRVTKKDVEAYIPGKSKPAVQQVVIPEVIGEERYEEVTISQMRKTIAKRLS
                     DSKYTSPHFYLTMEINMEKCISARNALNEISPVKISFNDIIIKASAAALRQHPKVNSS
                     WLGDKIRYNKHIHIGVAVAVDEGLLVPVVRFADGKTLSQISAEVKDLGVKAKSKQLQP
                     KDWEGNTFTISNLGMFGIEEFTAIINPPDACILAVGGIKETPVVKNGEVVPGNVMKVT
                     LSCDHRVVDGAIGSAFLQTLKQMLEEPIRILV"
     gene            complement(2348..5281)
                     /locus_tag="VGA21_07005"
     CDS             complement(2348..5281)
                     /locus_tag="VGA21_07005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650289.1"
                     /translation="MNFKRAVFLGWILFSGFSLKAQELESYPASIKWWQINTPHFKII
                     YPESGAKQAGRIANALEHFYGPASKSLNTSPRKIRVVVNNQTSVSNGFVSMAPRRSEI
                     FTAPPQDHNLVGSNEWIPFVTLHEYRHIVQFQKAKTGFSKLSYMLFGPEAFNAMAVLA
                     TPTWFWEGDAVVIETSMSRSGRGRNPEFDIEFRTPLLTNDKPYSYYKQYLGSYRDFIP
                     DHYRIGYFMSTYLRNKYGRQIWDPILKQANAHSFLPFTFSIAMKRNTGKYLLPIFKEM
                     MSEMKSKWQENLVEEEEVEIFSNENRKVFTNYLYPQHLDDTTILVVREGLGNIQQFIT
                     MSPSGEEKQIFTPGVLNRSPMISAKKGLIVWTEHVPDIRWSKRTFSSVKIYNNATKLV
                     TRLTINENFASADISYDLTKIVSVYTSEEGKQQIAILQSATGTVIREFPNPDNSFYQH
                     PRFMEDDKSVIVVKLNEEGKSIVLLDVETGIETQLTFPSAENVGYPVQNGEYVFYNSP
                     YNGIDNIYAVEISNGKIFQITEAKFGAYNPHVSPDGEYLYYNLFQANGMDIVRKELDP
                     SSWIPLEVVKDRNIDINGSIVQQEDNPDLLNTIPETQFESKRYRKFSGIFTPVSWGPY
                     LDATTSELFIGVRSRDVLSNIAWDAGYIYNNTEGTESWRGGFSYQGIYTPINSSIIYG
                     KRYETVVYLDLADTLNPNKITELSWYETEVETGLSLPLNLTNGRNFTGLTFENSVSYI
                     HKEGYQNPNENPDNFLIFPVSQGNGNIISNNFSIFFSRLWRRSKRDINSKFGQYFIFR
                     TSGTIAGDYTGNITTFRAGLFFPGFFRHHSLFLRGGVQSQKLSLDSDFYRFRNQIEFP
                     RGFGASSFNNYSYGSVTYAMPLLYPDLAVGPVVYIQRIKANLFVDFARGTRESVRIPD
                     RNLAFPPFTVDYLSYGAEITFDFNLFRLLYPLEMGVRFNFMNDPFGSGQDPFSYEFLI
                     GSFGF"
     gene            5457..8351
                     /locus_tag="VGA21_07010"
     CDS             5457..8351
                     /locus_tag="VGA21_07010"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX9650290.1"
                     /translation="MKLPNKIFKNSLWLVIPLIVFACVPAKDESAGNNLRIDFEKYVL
                     PNGLNIVLHEDHSDPIVSTAILYHVGSNRETKGRTGFAHLFEHMLFQESENVDQDQFF
                     KKIQDAGGTLNGGTWQDGTIYYEVVPKNAMEMVLWLESDRMGYFINTVTEGAFVNQQE
                     VVQNEKRQRVDNNPYGHTNYVIHKAMFPDGHPYNWQVIGELEDLQNATVEDVKEFYRN
                     FYGPNNATLVLAGDFNKDEAKALIEKYFGEIEASGNTGESLALKSLEPMNVAIDETIR
                     LYHQDNFAKVPQLNMVWAAVHSNHPDSYPLNFLAQLLSQGKKAPLYKVLEKEKELTSD
                     PQAFNDAMELAGKFQIRINANDGVDLDDIEEGIFEAFKRFETEGITDKDVERVKASLE
                     TNFYNGISSILGKSFQLASYNAFNGDPGYIEQDIENILAVTKDDIIRVYNLYIKDKPF
                     ILTSFVPKGQMDLMAENSNPAQVKEEAIVENVDAQVAESDVEVEKTPSEIDRSAEPPK
                     GEQPGLQIPTSWTVTLDNGMEVYGIRQDELPLVNFNLVIKGGHMLDDFGKIGVANLMT
                     DIMQEGTKNKTPEELEEEIELLGANINMFTSDGAITISGNTLARNFEKTLDLVEEMLL
                     EPRWDEEEFGRIKTSTVNQVIRREANPSAIAGDVFDRILYGDENMRGYNTLGTPENIN
                     SITIDDLKGFYDNYFSPSVTNFHVVGSVTQERVMDALKDLELKWAARDVTIPEYAVAE
                     QTDNAKLLFYDVPDAKQSVINIGYLALKRTDPDYYPVQVMNHGLGGSFNSRVNLVLRE
                     EKGYTYGARTFFDGTDIVGAFNASSSVRTNVTLESVKIFREEMDKYREGISEDDLEFT
                     KNALIKSNARRFETQGSLLGMLQTMSAYNFGSDYIKKEEDIVRNMTLEQHKGLAQKYL
                     IPSKMIYLIVGDARSQFPRFADQGFDVVALVDKEGKVKGDKISIEVNQQD"
     gene            complement(8422..10062)
                     /locus_tag="VGA21_07015"
     CDS             complement(8422..10062)
                     /locus_tag="VGA21_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007658964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent hydrolase"
                     /protein_id="HEX9650291.1"
                     /translation="MFRNSIFACTLLLIMQCTPPNEAPNENEDTETNVEMRLSEYARV
                     PLTADLSSLTENQRKMIPILIDAAAIMDDLFWKEAYGNKAELLSSINDENAREFAKIN
                     YGPWDRLRGDEPFIDGFGSKPAGAQFYPEDITKEEFESLSAEDKKSLYTMVRRAEDGS
                     LITIPYYEFFKEEVTRASDLLRMAAEFAENDELKRYLTLRAAALLSDEYQPSDFAWME
                     MKTNVIDVVIGPIENYEDKLFNYKAAHEAYILIKDVQWSERLSRFANFLPRLQQELPV
                     PAAYKQDKPGTESQINAYDVIYYAGDCNSGSKTIAINLPNDVEVQLQKGSRRLQLKNA
                     MRAKFDKIMVPISELLIAADQRSHVNFDAFFANTMFHEVAHGLGVKNTITDKGSIREA
                     LKEKYSALEEGKADILGIYMVTQLHNMGEVEGDLMDYYVTFLAGIFRSVRFGATSAHG
                     QANMIRFNYFAEMDAFSKDDNGFYSVNIENFQKAMAGLSEKILTLQGDGDYEGTAALF
                     NEKGMVRQDLQNDLNRVSNAGIPRDIVFDQGVEILGLK"
     gene            10182..10772
                     /locus_tag="VGA21_07020"
     CDS             10182..10772
                     /locus_tag="VGA21_07020"
                     /inference="COORDINATES: protein motif:HMM:NF025380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4230 domain-containing protein"
                     /protein_id="HEX9650292.1"
                     /translation="MLKLKTPLIIILILLGVTAGFILSKGLATKVLSPPLVFRLNEIK
                     KVRQLKLVTYHFEEIIPIEKNGKIKLLLIVPATVSGYIDLDALQYRIFNDTLISVSLP
                     SASVDSANFQIENAVNYDLEKRFSINLGTGLYQEVFEELKEKLKISKINVTQKAFELG
                     LESSTNNAAKEYISALLSDLEYVVEFKDKNLLNYSD"
     gene            complement(10752..11579)
                     /locus_tag="VGA21_07025"
     CDS             complement(10752..11579)
                     /locus_tag="VGA21_07025"
                     /inference="COORDINATES: protein motif:HMM:NF025420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4249 family protein"
                     /protein_id="HEX9650293.1"
                     /translation="MRKIYTLIMMIVTLSCDEVIQLDPEQTDQKIVIEALVTDDPDLN
                     YVRITSNVDFYYQGKIPGISGASILVLDDIGNVYDFVEYDTSNPDSSGYYFPQPAFTG
                     IINNTYTLDVTLNGITYSATEPMLPNFELDSLSVRKDPDPGQDDIEDNLLYNVLFYGF
                     EPQETKDYYYFEFFANDELLRNDADVFISDDIFLAGKISGLEFPFNYALGDSATIRVF
                     SISRKVYLFYSELASVLGNDGGMFSPPPANPLTNLSNGALGVFQVSSVKKKSIRVIE"
     gene            complement(11581..>13138)
                     /locus_tag="VGA21_07030"
     CDS             complement(11581..>13138)
                     /locus_tag="VGA21_07030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX9650294.1"
                     /translation="SFLVSGRRSYADIFIAAAGEDARVSFYDINAKANWRLNNNNRFF
                     AAFYVGKDSFSFNKIFGFDWGNRTATLRWNHHYTEKLFSNLTLIGSQFAYGLELKDPV
                     QGFEWTSRIQQFTLKADNHFFASPKVEIKFGYDLSYRRFFPGKIEPNQPGSIFTTSEL
                     ERQFALDHALYLESKQTISERFTLELGVRLSIFQNIGETTIRYYEDINNRSSTTVTGE
                     RKYDQFENIKTFVNPEPRVSGRFLLNSQSSLKASYNRMVQNVHLVSSGTVPLPLNTWT
                     PSNPYLKPQIADQFAVGFFRNFRSNTIEFETELYYKNISKITDFADNAMIFFNEDLAT
                     EFRQGKSTSYGWELMFNKKEGKLNGFISYTLSKTTRKVSDVNQGRTFLANYDRRHNVS
                     ISGVYELNRAWSFGLNFVYNTGRPTTIPTGRYQYKYYSADYFSERNSYQLPDYHRMDI
                     SATLTPGREKPRKLRSKWVFALYNVYNRKNPFSLFTEDRFESGEVVGKQARMIYLFPI
                     LPSITYSVEF"
BASE COUNT         3905 a         2731 c         2517 g         3985 t
ORIGIN      
        1 aattatttca atgttcttct ccctcttttc aagcaaggcg agcgtttgcc tgcttttcat
       61 gcacctggga ttatggtata tggtaaccat caagagtgat tgttagagcg taccgtaact
      121 ataatttctc aatcacaaaa ttatgattcg tataaataca aatatccgcc gctatggtca
      181 ggctttcttt tactatttct tcggcagata aattccctga gaatttttta agtgccatgg
      241 ctgctgactg cgcatacatg cttcctgaac caattgctgc aatatcatga tcgggttcta
      301 taacatcccc cgttccggac aagatcaaaa tttcttcttt atttgccact atgagcattg
      361 cctccaatcg ccgcaggaat ctgtccgttc tccagtcctt agctaattcg atagccgctc
      421 ttttcatatt tccaccgtag ctgcttagct tttcgtcaaa tctttccaat aaggtaaagg
      481 agtcggcagt agaacccgca aagccggcaa tcacttttcc atcctggagc ttgcgaattt
      541 ttctaacgtt actttttgcc acataatttc ccatggtagc ttgtccatcg gcaccaatag
      601 caacgctatc catgtgacga accgctatga cagtagtggc atgtatctca gtttttttca
      661 tttttataca agaattctga tcggttcctc cagcatttgc tttagagtct gaagaaacgc
      721 cgacccgatg gcaccatcaa caacgcggtg atcacaggaa agtgtgactt tcattacgtt
      781 gccaggcacg acttccccat tttttaccac cggagtttct ttgattcccc ccaccgcgag
      841 gatgcaggca tccggcggat taataatcgc ggtgaattct tcaataccaa acatccctaa
      901 atttgaaata gtaaacgtgt taccttccca gtctttcggt tgaagttgtt tgctttttgc
      961 cttcacccct aaatccttca cctcagctga aatttgtgac aaggttttac catcggcaaa
     1021 acgaactaca ggaaccaaaa gtccttcatc cactgccacc gctacaccaa tatgaatatg
     1081 cttattatag cgaatcttat cacctaacca ggagctgtta acttttggat gttgtctcag
     1141 cgcagcagct gatgctttaa ttatgatatc attaaatgaa attttaaccg gagatatttc
     1201 attgagcgca tttcttgcag aaatacactt ttccatatta atctccatgg ttagataaaa
     1261 gtgtggggaa gtatatttac tatctgacaa gcgctttgca atggttttgc gcatttggct
     1321 gattgtaact tcttcgtatc tttcttcacc aatcacttca ggtattacta cctgctgtac
     1381 ggcaggtttt gattttccag gtatataagc ttcaacatct ttctttgtta cgcggccatt
     1441 atcaccggtt cctgttatca aattaatgtc atatcctttg tctttcgcca ttttcctggc
     1501 caatggagaa gctaaaattc ttccgttggc caatccctgt ttttctggat ttgtttcctg
     1561 tatttgctca acaggagctg attcagcaaa aggcggagcc tgcgttttaa ctgttgctga
     1621 aactgaaata gtcgctttct gtttatgagc ctttattaga gattggtaat cggcaccttt
     1681 ctcaccaatt acggcaatca cgccatctac ttccaccgat tccccggctt taactccaat
     1741 ataaagtaaa gtaccgtcct cataggcttc gagttccatg gtcgctttat cggtttcaac
     1801 ctcagctatt atgtcgcccg attttacagt gtctcccacc ttcttcaacc aatttgcaat
     1861 tgtaccctcc gtcatggtat cactcatttt gggcatggta attactgaag cctttatatc
     1921 agaggtgtcc ctttcttctt catttttttt tgaagtttct tttccatcca tcccaagttt
     1981 cttatcatcc ttcccgttgg aagatgcatt ccctccttca tccttcagga gatcatcaaa
     2041 actctctcct tttttaccaa ttacggcgat aattccattc acaggcgcgg cttcaccatc
     2101 cttaattccg atgtgcaata aggttccatc ttcataagct tccagttcca ttgtggcttt
     2161 atctgtttct acttccgcga gtatatcgcc ggcctttact tcatcaccaa ccttcttcag
     2221 ccattttgca atgacacctt cttccattgt gtcactcatt ttgggcattc tgataacttc
     2281 agccataaat tgggtgtttc ttcaaaaata cctgtaaata tatctaattt caagctagcg
     2341 gaatgctcta aaacccaaaa cttccaatca ggaattcgta actaaagggg tcttgtccgg
     2401 agccaaaggg gtcgttcatg aaattgaagc gaactcccat ttccaacgga taaagcaacc
     2461 ggaataaatt aaagtcaaaa gttatttcag ctccatagga taaataatca actgtaaatg
     2521 gtggaaaggc aagatttctg tccggaattc ttacagattc tctggttcct ctagcaaaat
     2581 ccacaaacag gttggctttt atcctttgga tatagactac aggaccaaca gcgagatcag
     2641 gataaagcaa aggcatggca taagtgactg agccataaga ataattattg aatgaagatg
     2701 ctccaaagcc tcttggaaat tcaatctggt ttctgaatcg atagaaatca gaatccagtg
     2761 aaagtttctg agactgaact ccaccgcgaa gaaatagaga atgatgtctg aaaaaccccg
     2821 ggaagaacag gccggccctg aatgtagtaa tgttacccgt atagtcaccg gcaatggtac
     2881 cggaggttct gaaaataaaa tactgtccga atttgctatt gatatcgcgc ttgctccgcc
     2941 gccataaccg ggaaaaaaag atactaaaat tattggaaat gatattacca tttccctggg
     3001 acacaggaaa tataagaaag ttatcaggat tctcattagg gttttgatat ccctccttat
     3061 ggatataaga cacggaattc tcaaatgtta ggcctgtaaa atttcttcca ttggtgagat
     3121 ttaaaggaag cgacaaaccg gtttccactt cagtttcata ccaggataat tcagtgattt
     3181 tgtttgggtt tagtgtatca gccagatcaa gataaacaac tgtttcatat ctctttccat
     3241 atattattga agaattaatg ggcgtatata taccttgata gctaaaaccg cccctccagc
     3301 tttcagtacc ttcggtatta ttatatatat atccggcatc ccaggcaatg ttgcttagta
     3361 catcccttga acgaactcca ataaataatt ctgaagtagt ggcatccaga tagggtcccc
     3421 agctaacagg agtaaatata ccactaaact ttctgtagcg ctttgattca aattgagtct
     3481 caggaattgt attaagaagg tcaggattat cctcctgctg aacgatagac ccattaatgt
     3541 caatattcct gtccttcacc acctctaacg gtatccatga agaagggtcc aattcctttc
     3601 ttacaatgtc cattccattc gcctggaata aattatagta aagatactct ccatccggac
     3661 ttacatgcgg gttgtaagct ccaaatttcg cctctgttat ctgaaatatc ttaccgttcg
     3721 atatttctac tgcgtaaata ttatcaatac cattgtaagg cgagttataa aatacatatt
     3781 ctccgttctg aacgggatat ccaacgtttt ccgcggatgg aaaagttaat tgcgtttcga
     3841 tcccggtttc aacatcaagc aatacaatac ttttcccctc ttcattcaac ttcaccacaa
     3901 ttacactttt gtcatcctcc ataaatcgcg ggtgctgata aaaactgttg tccgggttag
     3961 gaaattccct gataacggtc ccagtcgcgc tttgaagtat agcaatttgc tgtttccctt
     4021 cttccgatgt atacacggag acaatttttg tgaggtcata actaatgtct gctgaagcaa
     4081 aattctcatt aatggttaac cgggttacca actttgttgc attattatag atttttacag
     4141 aagaaaaagt ccgcttagac cagcggatat cgggtacatg ttctgtccat acgatcaaac
     4201 ctttttttgc cgaaatcatt ggtgatcgat tcaaaactcc aggcgtgaaa atctgtttct
     4261 cttctcctga aggagacatt gttataaatt gctgaatgtt gccaagtcct tctctgacaa
     4321 caagaattgt ggtatcatca agatgttgtg gatagaggta gttggtaaaa actttgcggt
     4381 tttcattaga aaaaatttca acttcctctt cttccactaa attttcctgc catttgcttt
     4441 tcatttcact catcatttcc ttaaaaatcg gtagcaaata tttgcctgta tttctcttca
     4501 tggcgataga aaaagtaaag ggaaggaatg aatgagcatt tgcttgttta aggatgggat
     4561 cccatatctg tctaccgtac ttatttcgca aatatgttga cataaaataa ccgatccggt
     4621 aatggtctgg aataaaatcc ctgtaagatc ccaggtattg cttgtagtag gaatacggct
     4681 tgtcattagt cagcagcggt gttctgaact caatatcaaa ttcaggattt cttcctctgc
     4741 cgcttcggct catcgaggtt tcaataacaa ccgcatctcc ttcccaaaac catgttggtg
     4801 ttgcaagcac tgccattgca ttaaatgcct caggaccaaa aagcatataa ctcagcttac
     4861 taaatccggt cttagctttt tgaaactgaa cgatatgcct gtactcgtgg agagttacga
     4921 atggtatcca ttcattgctt ccaactaaat tgtgatcctg gggaggtgca gtaaaaattt
     4981 ctgaccgcct tggagccatc gagacaaaac cattggaaac tgaagtctga ttatttacta
     5041 cgacgcgtat ctttcgaggt gatgtattca ggctttttga cgccggacca taaaagtgtt
     5101 caagtgcatt ggcaattctt ccggcctgtt tagcacctga ttctggatag ataattttga
     5161 agtgtggtgt atttatttgc caccatttta tggaggctgg gtaactttcc agttcctggg
     5221 ccttcaggga aaaaccggaa aaaagtatcc atcccagaaa aacagccctt ttaaagttca
     5281 tagaagtcaa aacctagtaa aaacaatgcc aatatagttt gattaatgat aattattctt
     5341 agtaaaaatc aacggtgaaa ttttaatgcc cgataacaat ggtattttta tgaatggttt
     5401 taataaaagg ttcaaaattc ataattttga tgccttcata acaaaaaata agaatcatga
     5461 aactaccaaa caagattttc aaaaattcat tatggctggt tattccgctc attgtatttg
     5521 cctgtgtacc tgcgaaagat gagtctgcag gtaataatct gcgaattgat tttgagaagt
     5581 acgttttgcc taacggctta aacattgtcc tgcatgaaga tcattcagat ccgattgttt
     5641 caactgcaat tttatatcat gtaggttcta atcgcgaaac taaaggccga actggttttg
     5701 cacatctttt tgaacacatg cttttccagg aatcagagaa tgtagaccag gatcaatttt
     5761 ttaagaaaat tcaggacgcc ggaggcacat taaatggtgg aacctggcaa gatggaacca
     5821 tctattatga agtggtacct aaaaatgcca tggaaatggt attatggctt gagtctgacc
     5881 gtatgggata ctttataaat acagtaacgg aaggcgcatt tgtaaaccaa caggaagttg
     5941 ttcaaaatga aaagcggcaa agagtggaca acaatcccta tgggcatacc aattatgtaa
     6001 tccataaagc aatgttccct gatggccacc cttataactg gcaggttatt ggtgaactgg
     6061 aagacttgca aaacgccacg gtagaagatg ttaaagaatt ctaccgtaac ttctatggac
     6121 ccaataatgc aaccctggtt ctggccggag attttaataa ggatgaagca aaagctttaa
     6181 tagaaaaata ttttggagaa attgaagcga gcggaaacac cggagaatca ctcgctctaa
     6241 aatctctgga acctatgaat gttgctattg atgaaacgat tcgtttgtat catcaggata
     6301 actttgcaaa ggttccccaa cttaacatgg tttgggcagc agtacatagc aatcatcctg
     6361 actcctatcc actaaacttt ctggcgcagt tgctatcaca gggaaagaaa gctccgctat
     6421 ataaagtttt ggaaaaagaa aaagaactta cctctgatcc acaagcgttt aatgatgcaa
     6481 tggaattggc cggcaaattt cagatacgaa taaacgccaa tgatggcgtt gatctggatg
     6541 acattgaaga aggtatcttt gaggcgttta aaagatttga aacagaaggc attacggata
     6601 aagatgttga aagagtgaag gcaagtcttg aaaccaactt ctataatggg atcagcagta
     6661 ttttaggcaa gtctttccaa ctggccagtt acaacgcatt taatggtgac ccgggataca
     6721 ttgagcagga tatcgaaaat atccttgcgg taaccaagga tgacattatt cgagtatata
     6781 atctttatat caaagacaaa ccttttattc ttaccagctt tgttcctaaa ggtcaaatgg
     6841 atctcatggc agaaaatagc aacccggccc aggtaaaaga ggaagccata gttgaaaatg
     6901 ttgatgcaca agtggccgag tctgatgtag aggtcgaaaa gactccttca gaaatcgacc
     6961 gaagcgccga gccgccaaaa ggagagcagc caggtttgca aattccgacc agctggactg
     7021 tcacactgga caatggaatg gaagtctatg gcatcaggca agatgaactg ccattagtga
     7081 attttaatct ggttattaaa ggtggccata tgttggatga ttttggcaaa attggtgtgg
     7141 ccaatttgat gacggatatc atgcaggaag gaacaaagaa taaaacccct gaagagctgg
     7201 aagaagaaat agaattgctg ggagccaata ttaatatgtt taccagtgat ggagccatca
     7261 caatttcagg taatacgctg gcaagaaact ttgagaaaac tcttgatttg gttgaagaga
     7321 tgttactgga gccgagatgg gatgaggaag aattcggcag aattaaaaca agtactgtta
     7381 atcaggtaat tcgccgggag gctaatccct ccgcaatagc cggtgatgtc tttgacagga
     7441 tattatatgg tgatgaaaac atgaggggat ataatacact tggcactccg gagaatatta
     7501 attccataac cattgatgat ttaaaaggat tttatgataa ttatttctct ccatcagtaa
     7561 cgaatttcca tgttgttgga agtgtaaccc aggaaagagt gatggatgcc cttaaagatt
     7621 tagaactgaa atgggccgcc agggatgtta cgattccgga gtatgcggta gcagagcaaa
     7681 ccgacaatgc caagttgtta ttttatgatg ttccggatgc aaagcaatcc gtaataaaca
     7741 ttggttatct cgccctgaaa cggaccgatc ctgactatta cccggttcag gtgatgaatc
     7801 acggattggg tggctcattt aatagcagag taaatttggt attaagggaa gagaagggtt
     7861 acacctacgg agcaagaact tttttcgatg gaactgacat cgtaggcgct tttaatgctt
     7921 catccagtgt aagaactaac gtaactctgg aaagtgtgaa aatttttagg gaagaaatgg
     7981 ataaataccg cgaaggaatt agtgaagatg atctcgaatt caccaagaac gccttaatta
     8041 aatcaaatgc cagaagattc gaaactcagg gtagcctgct gggcatgcta cagactatga
     8101 gcgcgtataa ttttggttct gattacatta agaaggaaga ggatattgtt cgtaacatga
     8161 cactggagca acataaagga cttgcacaaa aatatctcat tccatcaaaa atgatttacc
     8221 tcattgtcgg tgatgcaaga agtcaattcc caagatttgc tgatcagggc tttgatgtag
     8281 tcgcactcgt tgataaggaa ggaaaggtta agggagacaa aattagtatt gaagtaaacc
     8341 agcaggatta gatccctccg gacaataaaa aaagaaatgc ctttctccaa acggaggaag
     8401 gcatttttct tatcaggtaa attattttaa gcctagaatt tccacaccct ggtcaaaaac
     8461 tatatctctg ggaatccccg catttgaaac tctgttcaaa tcattttgca aatcttgcct
     8521 aaccattcct ttttcattga acaaagcagc agtaccttca taatcaccat caccctgcaa
     8581 agtcaggatc ttctcagaca gaccggccat agctttttga aagttttcaa tatttacact
     8641 atagaaacca ttatcatctt ttgagaaggc atccatttct gcaaagtaat tgaacctgat
     8701 catatttgcc tggccatgag cactggtggc tccaaaacgg actgatcgga aaatacctgc
     8761 aagaaaagta acatagtaat ccataaggtc accctcaacc tcacccatat tgtgaagctg
     8821 ggtaaccatg tatataccca ggatatccgc ctttccttct tccaatgcac tgtatttctc
     8881 cttcaatgct tcgcgaattg atcccttatc ggttatcgtg tttttaacac cgagcccatg
     8941 tgcaacctca tgaaacattg tgtttgcaaa aaacgcatca aaattaacat gagaacgttg
     9001 atccgcagca atcagaagtt ccgagatggg taccattatt ttatcaaact ttgcccgcat
     9061 ggcatttttt aattgtagtc ttcttgaacc tttttgtaac tgcacctcta catcattggg
     9121 taaattaatc gcgatagttt tactgcctga attacaatct ccggcgtaat agatcacatc
     9181 ataagcatta atttgtgatt cggtgccggg cttatcttgt ttataggcag ccggtaccgg
     9241 aagctcctgc tggagtcgcg gaagaaaatt ggcaaacctc gacaatcttt cgctccactg
     9301 aacatctttt atcaggatat atgcttcgtg cgccgccttg taattgaata atttatcttc
     9361 gtaattttca atcgggccaa tcactacatc aatgacgttg gttttcattt ccatccaggc
     9421 gaaatcactg ggctgatatt catcagaaag caaagcagct gcccgcaagg ttagatatct
     9481 cttcaactca tcgttttcag cgaactccgc agccatccgg agtaagtcac tcgctcgcgt
     9541 tacctcttcc ttaaaaaatt cataataagg aatagtgata aggctgccat cttccgctct
     9601 tctaaccatt gtataaaggc ttttcttatc ttctgcagaa agggattcaa attcttcctt
     9661 tgtaatatct tcaggataaa attgagcacc tgcgggtttt gaaccgaaac catcaataaa
     9721 tggctcatct cctcttaatc tgtcccaggg tccataattt attttagcaa attcccgggc
     9781 attttcatcg ttgattgaac ttagaagttc cgctttgttt ccataggctt ctttccaaaa
     9841 aagatcatcc ataatagctg ccgcatctat cagaattggg atcattttcc tttgattttc
     9901 agtcagagaa gataaatccg ccgtaagcgg cacgcgagca tattcactta gcctcatttc
     9961 aacattagtt tcggtgtctt cattttcatt gggtgcttca ttgggtggtg tgcattgcat
    10021 gattagtaat aatgtacatg cgaatattga attcctgaac atggccttta agattttttg
    10081 tttaacaatt taagcaattt aacggtggca ttatttttca gtaaatgtat aatactttta
    10141 attaattata ttatttttat attttatatt atttttcata tatgttgaaa ttgaagactc
    10201 ccctgataat tattctgatt ctcttaggtg ttactgccgg attcattttg tcaaaaggct
    10261 tagctacaaa ggttctttca cctcccctgg ttttcagact taatgaaatc aaaaaggttc
    10321 gacaactaaa attagttact taccattttg aagaaataat tcctatagaa aaaaatggga
    10381 agattaagct attgcttata gtacctgcaa ctgtttccgg ttacatagat ttggatgctt
    10441 tgcaatatcg cattttcaac gatactctta tctcagtttc cttgccttcc gccagcgttg
    10501 acagtgctaa tttccagatt gaaaatgcag tgaactatga tttggaaaag cgatttagca
    10561 taaacctcgg cacaggcctt taccaggaag tttttgagga actgaaagaa aaattaaaaa
    10621 tcagcaaaat aaatgtaacc caaaaagcat ttgaactagg acttgaaagt tcgacaaata
    10681 atgcggccaa agaatatatc tccgccttgc tatcagactt agaatacgtg gtcgaattca
    10741 aggataaaaa tttactcaat tactctgatt gatttcttct tcacagagga aacctgaaaa
    10801 acacccagcg caccattgct caggtttgta agcggattag caggaggtgg tgagaacatg
    10861 ccgccatcat tgcccaggac actggcaagt tcagaataga acaaataaac ctttctgctt
    10921 atactgaata cccgaattgt tgcggagtct cccagggcat aattaaatgg aaattctaat
    10981 ccggaaatct taccggcgag gaaaatatcg tcacttatga atacatccgc atcgttccta
    11041 agcaattcat cattggcgaa gaattcaaag taatagtaat ctttagtctc ctggggttca
    11101 aatccgtaaa acaaaacgtt gtataataaa ttatcttcaa tatcatcctg gccggggtct
    11161 ggatctttcc tcactgataa actatccagc tcaaaattgg gcagcatggg ttcagtggca
    11221 gaataagtaa ttccattcaa tgtaacatcc agcgtgtagg tattgtttat tattccggta
    11281 aaagccggct gaggaaaata atatccgctg ctgtccggat tagaggtatc atattcaaca
    11341 aaatcataaa cattcccaat gtcatctaaa accaatatac ttgcaccgct tattcccggt
    11401 atttttccct ggtaataaaa atcaacgttg gatgttatcc gcacataatt taaatccgga
    11461 tcgtccgtaa ccaatgcttc aattacaatt ttctgatctg tttgttccgg atctagctga
    11521 atcacttcat cgcaagacaa agtaacaatc atcattatta gtgtataaat ctttctcatt
    11581 tcaaaattca acgctatatg ttatggatgg aaggatcggg aacaggtaaa tcatgcgggc
    11641 ttgtttcccc accacctcac cggactcaaa ccgatcttca gtaaaaagac taaacggatt
    11701 cttacggtta taaacgttat acagggcaaa aacccatttc gaacgaagtt tccgaggttt
    11761 ctctcttccg ggagtaagtg ttgccgaaat atccattcta tgataatcag gcaactggta
    11821 actatttcgc tcactgaaat aatcagcgct atagtattta tattgatacc ttcccgtagg
    11881 tattgtggtg ggcctgcctg tgttatagac aaagtttaaa ccaaatgacc aggcgcgatt
    11941 taattcgtac accccgctta tgcttacgtt atgcctgcga tcataatttg ccagaaaggt
    12001 tctgccctgg tttacatcag acactttgcg ggttgtttta gataaggtgt agctgataaa
    12061 tccatttaac ttgccttcct tcttattaaa catgagttcc cacccataag aagtagattt
    12121 accctgcctg aactcggtgg ccaaatcttc attgaaaaag atcattgcat tatccgcaaa
    12181 gtctgtgatc ttgctaatat ttttataata tagctcggtt tcaaattcaa tagtattgga
    12241 tctgaaattc ctaaaaaaac caaccgcaaa ttgatccgca atctgtggct taaggtaagg
    12301 attgcttgga gtccaggtat ttaatggcaa tggaacagta ccggagctaa ccaggtgcac
    12361 gttctgaacc attctgttat acgaagcctt taaagaactc tgggaattaa gaagaaatct
    12421 tcctgaaact ctgggctcag gatttacaaa tgtctttata ttctcaaact gatcatactt
    12481 cctttcccct gtcactgttg ttgaagacct gttatttata tcttcataat accttattgt
    12541 agtttcgcca atgttttgga atatgctcag tcgaactccc agctcaagcg taaatctttc
    12601 ggatatagtc tgcttgcttt caagatacag ggcatgatcc agtgcgaact ggcgttcaag
    12661 ttccgaagtt gtaaaaattg agccgggttg attgggttca atcttcccag ggaaaaacct
    12721 gcggtaagat aaatcatatc caaatttgat ctcaactttt ggacttgcga agaaatgatt
    12781 atcggctttc aaagtaaact gttgaattct ggaagtccat tcaaaccctt gtacagggtc
    12841 ttttaattcc agcccatacg cgaactgact gcctataaga gtaaggtttg aaaaaagttt
    12901 ttcagtataa tgatgattcc atcgaagtgt ggcggtacgg ttgccccagt cgaacccaaa
    12961 aattttatta aaactgaatg aatctttacc gacataaaat gcggcaaaga atctgttgtt
    13021 gttgttcaac ctccaatttg cctttgcatt tatatcatag aatgaaaccc ttgcatcttc
    13081 tcccgctgcc gcgataaaga tatctgcata agaccgacgg ccggaaacca gaaacgaa
//
LOCUS       DASWHX010000106        14759 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_1198187, whole genome shotgun sequence.
ACCESSION   DASWHX010000106 DASWHX010000000
VERSION     DASWHX010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 14759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14759
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1198187"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(215..1390)
                     /locus_tag="VGA21_07035"
     CDS             complement(215..1390)
                     /locus_tag="VGA21_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014021351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEX9650295.1"
                     /translation="MQKHLNLRNLLLIAAINVTITACSSNNLAKQPLGNYKIVEAFPK
                     LLFARPVDFQHAGDGSNRIFVVEQAGLISVFSNDEITEVKNVFLDISNLVDDGGNEEG
                     LLGLAFHPNYAANGFFYVNYTASNPNRTVISRFSVSANANLADPASEMVILEFTQPFS
                     NHNGGQISFGPDGYLYIATGDGGSGGDPQGNGQNLSTLLGKILRIDVNNQSGINAYSI
                     PQDNPFANNSSGFREEIYAYGLRNTWRFSFDPVTGWIWAGDVGQNAYEEIDIIEKGGN
                     YGWNIMEGNHCYEPPSNCNETGLKGPVWEYGRSEGISVTGGFVYRGSDFLEFMNSYIY
                     ADYGSGKIWALDFSDLNNPVNSVLIASALNISSFGIDQNHELYLCAFDGKIYKLAGE"
     gene            complement(1450..3087)
                     /locus_tag="VGA21_07040"
     CDS             complement(1450..3087)
                     /locus_tag="VGA21_07040"
                     /inference="COORDINATES: protein motif:HMM:NF013109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HEX9650296.1"
                     /translation="MNPIFKPKLFTLLRDGIDKKQISQDILAGVVVGIVALPLAIAFA
                     VASGVSPEKGLITAIIGGFLISFLGGSRVQIGGPTGAYIIIVYGILTQYGINGLMIST
                     ILAGVILIIFGVLRLGALLKYFPHPLIVGFTSGIALVIFSTQIKDAFGLTIRTLPANF
                     VEKWIVYWNSMHTINFTALAVALITILITIFFRRITTKIPGSIIAIILITVGVQLMKI
                     PVPTIETFFGDIPNSFSITIPKIDLASLTVYLPLALTIAILGGIESLLSAVVSDGMIS
                     GKHRSNTELIAQGIANIVTPFFGGIPVTGAIARTATNVKNGGRTPVAGIIHALTLLLI
                     MVFLGKWAKLIPMSCLAGILIMVAYNMSEWRSFKAIMKGSIFDVIVLLTTFFLTVLVD
                     LTIAIEIGVVLSALLFMKRMSDISDRRLNHVVDTDLIEDYSNLPAGVSVFEISGPLFF
                     ASAKRYSEVMEEIGITSKILIIRMRHVAFVDQTGMHNLKETIKMLRSKGVLIILSGVN
                     LSVTEDFEKFGIVDLVEKSHVCDSFAKALKLANELAA"
     gene            complement(3274..4317)
                     /locus_tag="VGA21_07045"
     CDS             complement(3274..4317)
                     /locus_tag="VGA21_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015028294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline iminopeptidase-family hydrolase"
                     /protein_id="HEX9650297.1"
                     /translation="MKTIILKLEFGIACCLVLSCAGKIQEQEKNSLPYYFESQEAGIK
                     TGGIKVIPIETPKGTFNVWTKRIGNNPDIKLLLLNGGPGATHEYFECFESFLPQQGIE
                     FIYYDQLGCGNSDNPNDTSMWDLPRYVEEVEQVRTALNLTKDNFFLLGHSWGGILAIE
                     YALKYQQNLKGVIISNMMASIPDYNKYAEEVLAPQMDPEILGKIMEIEDAGDYENPTY
                     MDLLMPYFYAVHILRLPVDQWPEPVDRSFSKMNRSLYVTMQGPSEFGASGKLEFWDIK
                     ERLKEIAVPALTIGAQHDTMDPIHMEWMAEQMPKGSYLHCENGSHMAMYDDQVTYVEG
                     IIKFMKSIGNTIN"
     gene            4570..4986
                     /locus_tag="VGA21_07050"
     CDS             4570..4986
                     /locus_tag="VGA21_07050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650298.1"
                     /translation="MKTRAIFILISCFFTWGCEDNYVSQNIYGTWEWVKTTGGLIGEL
                     TPENSGYSEIIEINQQGIKVFQNGALMETKTYQILEDGSDCILINLNNSADQRRIHFE
                     STEPDQISISYYMAENTVCNDCPTSYYIKVGDVNEK"
     gene            complement(5000..5713)
                     /locus_tag="VGA21_07055"
     CDS             complement(5000..5713)
                     /locus_tag="VGA21_07055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650299.1"
                     /translation="MEIRKSYLAIRKAIGILGLLLPALVIFFHGELLTSISHYYYARS
                     AVFFIGIMFSFGLFLISYRGYEKDTKTEKLSDNTITNIGGFAALMVIFFPTSCLGSGG
                     FSITCYSEGLVMFGHVNKYVSAVHLISAGLFLFIMGWISIYRFTKTKENVILSAEKKR
                     NNRVYKFCGFLVWISLGVLIFEFAGQLIWSDKFQLSDYDIILFETIAVMAFGISWLVK
                     GDAIEDLIDIRRKYFRTGN"
     gene            complement(6050..7192)
                     /locus_tag="VGA21_07060"
     CDS             complement(6050..7192)
                     /locus_tag="VGA21_07060"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX9650300.1"
                     /translation="MKFTNLIFPCFQLLIATFLISCEREDLQVQTKYRYEKPSDLGDG
                     LSISDPITEGLDLMSLKVLMDSIDAGVYGGFHSMLILKNDKLVLEEYFNNWNQNDLHP
                     MYSVTKSFASAIIGITIDKGFISSEQETLSNLLPSFNNLAWTDEKKGITLKHFLTMSS
                     GFEWDEYGTPYDNPENPHYKMMTSSDWIKYLIELPMQYSPGEKYNYNTGSSTLFSVIV
                     EEATGEKFDEFAHNHLLTKLDINDYSWFYMPGDYPATGGSFGGLHLRSRDMAKFGLLY
                     MNDGKWGDEVVISQTWVELSLSPHINIVVSGDFTYGYQWWRNEKVKDKGGQLIQVPYG
                     VGLGGQYIFLIKEHNLLIIITSDNDPSNTLNNFAMIENYILNGVTN"
     gene            7366..8250
                     /locus_tag="VGA21_07065"
     CDS             7366..8250
                     /locus_tag="VGA21_07065"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEX9650301.1"
                     /translation="MIKLNGIFPPLPTSFDALENLATDKIKENIVNLSRYNLAGFLIL
                     GSNGELVNLTEKEIQEVYVASREAIRADKIMLAGTGAQSTRGTITNTQAAAAAGADAV
                     LVLNPSYYKSLMTTDALVAHYHAVADASAIPVLIYNMPANSGLDMDAETILKISEHEN
                     IIGLKDSGGNLAKMGNVKRMAKPDFQILAGSAGFLLPAFTVGAVGGILALANIAPQKC
                     ISIYEDYFSGNIEKAQKTQLELIPVNAAVTGKWGVPALKTAMDYMGLFGGTARKPLLP
                     LKEEIKKQVTDLIEKHVK"
     gene            8556..9170
                     /locus_tag="VGA21_07070"
     CDS             8556..9170
                     /locus_tag="VGA21_07070"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX9650302.1"
                     /translation="MKKLILLIPAMWIMSFTGTGPGITKAERKAAIESLNKTQDDLLA
                     TIEELSVEQLNFKSSPESWSVSECVEHIAISETNLFGMIQGTLKEQADAALKSEIKLT
                     DEQVFAAIVDRTYKVKTQEAFVPSGKFGSHEATIKEFLNKRKANIDYLKDTQDDLRNH
                     FFTFPVEALGTVDSYQLFYFMSGHTKRHTLQIEEVMSDPSFPKK"
     gene            9206..9556
                     /locus_tag="VGA21_07075"
     CDS             9206..9556
                     /locus_tag="VGA21_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020530034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEX9650303.1"
                     /translation="MKDYLLLFRGGEAHTERQNPEKWQTHMQKWMEWMRGLQEQGKLV
                     GAQPLDESGKQIKGTKKIVTDGPFMEGKEIVGGYLQCKADSYDQALEIAKGCPILEFE
                     SGIVEVREIQELKM"
     gene            9658..10938
                     /locus_tag="VGA21_07080"
     CDS             9658..10938
                     /locus_tag="VGA21_07080"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX9650304.1"
                     /translation="MPQSDVNILKLADHLFRNESKKMVAVLTKIFGTENLETAEDVVQ
                     DTLLHAINVWKIKGLPDNPSAWLFRVAKNKAIDIIRRNKHSVKYDFSIGETALLASEY
                     TLHTAMDNFWKEELIEDDLLGMMFVCCHPEISSENQITLILKTLCGFSTAEIAKTFLT
                     TNDIVSKRLYRTKEFFRVHKVALSIPSKEEIKKRTEVVLNSIYLLFNEGYNSTHNEYL
                     IRKDVIEEAMALCKMLSENKHTQIPEVFALLALMCFHAARLNSRLTRDGEIILLSEQD
                     RSTWDEALIEQGNEFMDKAAFGNSISSYHLEAAIAFEHCTAKTFESTNWNRILEYYNW
                     LCTLSPSPITELNKGVAIMQVHGAEAALRELNLIKERKKLESYYLYHSLIGEINSKMN
                     NTKQAIASFKLAMQLTNSEPEKRILHSKITALLN"
     gene            11356..11709
                     /locus_tag="VGA21_07085"
     CDS             11356..11709
                     /locus_tag="VGA21_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008630507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650305.1"
                     /translation="MNFVNYVYKKIYMWNINTPWFDVSVITCLVALGSIFLGHFETET
                     PKLRKVLKLLVTVAIMVTLSATVGRIWAFTFLGIMILAVLYIHIMWLPSKGINGWTGE
                     PRKKYYELRGWKWKE"
     gene            12255..12671
                     /locus_tag="VGA21_07090"
     CDS             12255..12671
                     /locus_tag="VGA21_07090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650306.1"
                     /translation="MDHHELHFYDQSREMDLICRYVDPERIEEVLETNFIEAGGWRHP
                     GKGESLPDTVSPGDNATVQFFIPYLKSLVEGDPATENWFFWLKRNSKLLELTLSKMQH
                     LQLKISPIREAKVILDLLGVTYDPSKYFQWLDKKVT"
     gene            13663..14337
                     /locus_tag="VGA21_07095"
     CDS             13663..14337
                     /locus_tag="VGA21_07095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650307.1"
                     /translation="MKKVILLVLTSSLSAFSFGQSKSLETGKQLPYSDRKLSVGINYA
                     IGNFYRLPTISSTKDFSGFAFGGIEIEKHLKNYLTLYIRSSLIINSSIPIPLGECFDD
                     CEYANVWENAAGLRRNFTVGKKSLINLGLGISLNHYLWSHNYSIQSTTDFIKVKDEFN
                     NTGIGFDSNINYFFRVGEGNINHFGIGFNYSPTFINSQLGNKYFYSIHFKLIYKHSFI
                     KRNRKE"
BASE COUNT         4702 a         2891 c         2840 g         4326 t
ORIGIN      
        1 ttgataattg ttcttttctg aaaaaattcc ctggtacctg attattatcc ctttcttgtg
       61 gtaactgatt ggattctcgg cggctttgca actttcgatt tatgaagact tcgggttggt
      121 atttgcagga ttgaccggtg ggtttatatt gttcatcaaa cacaaaaaaa tagttccggc
      181 tgatcaatct tttgtttaaa aatctgtacc aggactattc gccggcgagt ttgtaaattt
      241 ttccatcaaa agcacagagg taaagctcgt gattttggtc aattccaaat gatgaaatat
      301 ttaatgctga tgcaattaat actgagttga ccggattgtt taaatcagaa aaatccaatg
      361 cccatatttt tccagaacca tagtcagcgt aaatatagct gttcataaat tcaagaaaat
      421 cagatcctct gtacacgaat ccaccggtca cagaaattcc ttcactgcgc ccatactccc
      481 ataccggacc cttcaatcca gtttcattac aattggaagg cggctcataa cagtgattgc
      541 cttccattat attccagccg taatttcctc ctttttcaat tatgtctatc tcttcatagg
      601 cattctgacc aacatcgccc gcccaaatcc atcctgtaac gggatcaaaa ctgaagcgcc
      661 acgtatttcg caaaccatat gcatatatct cttcacgaaa tcctgaagaa ttatttgcaa
      721 atggattatc ctgaggaatt gaataagcat tgattccgga ttgattattt acatctatgc
      781 ggaggatttt gcccagcaaa gttgaaaggt tttgcccgtt tccctgcgga tcaccaccag
      841 atccgccatc accggttgca atatataagt aaccgtccgg accaaacgaa atttgaccac
      901 cattgtgatt gctgaacggt tgggtaaatt ctaaaatcac catttcactt gccggatccg
      961 caaggtttgc attcgctgaa actgaaaagc gggagataac cgttctatta ggattggatg
     1021 ccgtgtagtt tacataaaaa aaaccattgg cagcataatt cggatgaaaa gcaaggccta
     1081 acagaccctc ttcattaccg ccatcatcaa ccaggttgga aatatccagg aaaacatttt
     1141 ttacttccgt aatttcatca ttggaaaaaa ctgaaatgag tccggcttgc tccacaacaa
     1201 aaatccggtt gctgccatca ccggcgtgct gaaaatcaac aggacgtgca aacaaaagtt
     1261 ttggaaaagc ttctacaatt ttataattac caagaggctg ctttgctaaa ttgttagacg
     1321 agcaggctgt gattgtaaca ttgatagctg caattaataa aagattccgc aaattgagat
     1381 gtttttgcat cctatatgga gttgatgggg aatgaagaca tccaaaatta atgccgaaca
     1441 ataagattgt tacgcagcca gctcgttcgc gagcttcaga gctttcgcaa aagagtcgca
     1501 gacgtgagac ttttccacta aatctactat cccaaacttt tcaaaatctt cagttacgga
     1561 tagattaacg cctgacagga taatcaatac tcctttactg cgtaacattt taattgtttc
     1621 tttgagattg tgcattccgg tttgatcaac aaaggcaaca tggcgcatcc ggatgatgag
     1681 aattttactg gtaatcccaa tttcttccat aacttcagaa tatctttttg cagaagcgaa
     1741 aaataatggt ccgctaattt caaacaccga gacaccggca gggagattag aataatcttc
     1801 gatgagatcc gtatctacga catggtttaa tctccgatcg cttatatcag acattctttt
     1861 cataaataat aatgcggaca acactactcc gatttcaatg gcaattgtca agtcaactaa
     1921 gactgtcaga aaaaaggtag ttaaaagaac aatcacgtca aatattgaac ccttcattat
     1981 tgctttaaat gatcgccatt cgctcatatt ataggcaacc attatcagaa ttcctgccag
     2041 gcaggacatt gggataagtt ttgcccattt acccagaaat accattatca acaacagggt
     2101 taatgcatga ataatcccag cgaccggagt tctgccaccg ttcttaacat tagttgccgt
     2161 tcttgcaatg gctccggtta cggggatgcc accaaaaaaa ggcgtaacga tattggcaat
     2221 cccctgggca ataagctcag tattcgagcg gtgcttcccg ctaatcatgc catccgaaac
     2281 aacagctgac aataaagatt caatgccgcc aaggatggcg atcgtcaatg caagcggaag
     2341 atatacagtc aggcttgcta aatcaatttt tgggattgtt atactgaaac tgtttgggat
     2401 gtctccaaaa aacgtttcaa tagtaggaac gggaattttc atgagctgaa cacccacagt
     2461 aataagaatg atggctatta tggacccggg aatttttgtt gttatcctgc ggaaaaatat
     2521 tgtaatcaga atggtgatca aagcaacagc cagcgcggta aaattgattg tgtgcatact
     2581 gttccagtaa actatccact tctcgacaaa gtttgcaggc agggttcgaa tagtaagacc
     2641 aaatgcatct ttaatctggg ttgaaaaaat caccaatgca atcccgctgg taaatccaac
     2701 aattaaagga tggggaaaat atttaagtaa agctccaagt cttagcactc caaagataat
     2761 caggataact cccgccagga tggtggaaat catcagacca ttaatcccat attgtgttaa
     2821 aattccataa acgatgataa tgtaagcacc cgttggtccg ccaatctgga ctctgcttcc
     2881 gccaagaaat gaaatgagaa aacctcctat gattgctgtt atcagacctt tttccggcga
     2941 tacaccagag gcgacagcaa atgctatggc taaaggaagg gcgacaattc caaccacaac
     3001 tccagccagg atgtcctgtg aaatttgctt cttgtcaatt ccatcccgaa gcaatgtaaa
     3061 aagcttcggc ttaaagattg gattcattat ctaaacaact ggccacaaaa gtatgttgaa
     3121 ttttttcacg ccggctaaat ctgtcaaatt ttttctcgtt ttagataaac ccgtgagatg
     3181 atgccattgc aggaggcaaa gagtttcctc aacctcaatt ttggttaagc ttaagttgcc
     3241 gatactctga aggagattat agtggcaact tcgctaattg atggtgttgc cgattgattt
     3301 catgaattta ataattccct ccacataggt aacctggtcg tcatacatgg ccatgtgact
     3361 tccattttcg caatgcaaat aacttccctt tggcatttgt tctgccatcc attccatatg
     3421 tattggatcc atagtatcgt gttgagctcc aattgtcagt gcaggaacgg caatttcttt
     3481 aagcctttct ttaatatccc agaattccag tttgcctgaa gctccaaatt cgcttggacc
     3541 ctgcatagtt acatacaatg agcgattcat tttggaaaat gatctgtcca ctggctcagg
     3601 ccactgatca acaggaagcc gcaaaatgtg gactgcataa aaatatggca ttagcaagtc
     3661 catgtacgta gggttttcat agtctccggc gtcttcaatc tccataattt ttcccaggat
     3721 ctccggatcc atttgtggtg ccagtacttc ctctgcatac ttgttataat cggggatact
     3781 ggccatcata ttggaaataa tcactccctt aagattctgt tgatatttca atgcatattc
     3841 tatcgcgaga attccgcccc aggaatgccc cagcaggaag aaattatctt tggtgagatt
     3901 cagcgctgtc ctcacttgtt caacttcctc aacgtaacga ggcaaatccc acatagaagt
     3961 atcattagga ttatcggaat ttccacaacc cagctgatca taatagataa actcgattcc
     4021 ctgttgcggt aaaaaacttt caaagcattc gaaatactca tgagtggccc ctggtccgcc
     4081 atttagcagg agtaatttta tatccggatt atttccgatc cttttcgtcc acacgttgaa
     4141 cgttcctttt ggcgtttcaa taggaataac ctttattccg ccggtcttta tccctgcttc
     4201 ctgactttca aaataataag gaagggaatt cttttcctgc tcttgaatct tccctgcgca
     4261 ggaaagaacc aggcagcagg caattccgaa ttcaagtttc aaaattatgg tcttcataaa
     4321 attaatggtt gtttaaaatg aatttggtaa aaaagataac caaatcccat tccacgacta
     4381 catttttcta aatagccgga aagttcaagg catcaggact tccttgtagc attcgagtct
     4441 ttattttgga tgatgattag agacggttta tttttccctg aagttgaagc attgataatt
     4501 ttgaaaatat tattaattaa ggtacaaacc ttgcggacaa aagtggtgtc taatagtaaa
     4561 cagccaatta tgaaaacacg tgcaattttt attttgatta gttgcttttt tacctgggga
     4621 tgtgaggata actatgtaag ccaaaatatt tacggaacct gggaatgggt taaaactaca
     4681 ggagggttaa ttggggagct gacaccggaa aattccggat actctgagat tattgaaata
     4741 aaccagcaag gcattaaggt ttttcagaac ggagcattga tggaaactaa aacctatcaa
     4801 attctggagg atggttctga ttgcattttg attaatctaa ataattcggc cgatcaacgg
     4861 agaatacatt ttgaatcaac cgaacctgat caaatcagta tttcttacta catggcagaa
     4921 aatacagttt gtaatgactg cccaacgagt tactatataa aagttgggga cgtgaatgaa
     4981 aaatagtaaa tcgcgttttt taattgccag ttctaaaata tttcctcctg atatcaatta
     5041 aatcttcaat ggcatcccct tttaccaacc atgaaattcc aaaagccatc acggcaatag
     5101 tttcaaacaa aataatatca taatcagaca actgaaattt atcagaccat ataagttgac
     5161 cggcaaattc aaaaataagt acacccaggc taatccaaac caggaatccg caaaatttgt
     5221 acactctatt gttccgtttt ttttctgctg atagaataac attttctttt gtcttggtaa
     5281 acctgtaaat cgatatccag cccataataa acagaaagag tccggcactg attaggtgaa
     5341 ctgcactgac atatttatta acgtgaccga acataaccag cccttcagaa taacaagtaa
     5401 ttgagaagcc gccacttcct aaacatgaag ttggaaaaaa gattaccatc agagcggcga
     5461 atccgccaat gtttgtgatt gtgttgtcac ttagtttctc ggttttggta tctttttcat
     5521 agccacggta agaaatcagg aagagaccaa aagaaaacat aatcccgata aaaaacaccg
     5581 cagacctggc gtaataataa tgactgattg aagtaagaag ttcgccgtga aaaaagatta
     5641 ctaaagccgg taacaataaa cccaggatac cgattgcctt tcggattgcc aggtatgact
     5701 tcctgatttc cattttattt agattttgag ataaaagaaa ttcactattt caattcccgt
     5761 tctttcaacc tgaaattagt aaaatcttcc tctaatttta agcactaatc gaaattttta
     5821 tggtttatgg taaaattact atgccaaaat tctcaggaat cagaaacctg accatagttt
     5881 aggaaattag aaagaaatag actttgcaat aatggatgta ttttttttta aaaatgaatc
     5941 gtgggaaatc ttcttttcag tatattttga attacggaaa cagcaagaat ggtataacaa
     6001 actcttgctt gcttttcaaa caggaataag tatgtcacac ccgtgatcat cagttggtaa
     6061 ccccatttaa tatgtaattt tcaatcatcg caaaattatt caaagtgttt gaaggatcat
     6121 tatccgaggt aataatgatt agtaaattat gttctttgat taagaaaata tactgacctc
     6181 ccaggccaac accataaggt acctggataa gctgaccgcc cttatctttt accttttcat
     6241 tgcgccacca ttgatagcca taggtaaaat cgccggatac tacaatgtta atgtgcggag
     6301 aaagtgatag ttctacccat gtctgactaa tcacaacttc gtcgccccat ttgccgtcat
     6361 tcatatacaa aaggccaaat ttagccatat cccttgagcg gaggtgtaaa ccaccaaatg
     6421 acccacctgt tgccggatag tcgccgggca tataaaacca tgaataatcg ttgatgtcta
     6481 gctttgttaa cagatgatta tgcgcaaact catcaaattt ttctccggtt gcttcctcca
     6541 ctatcacgga aaacaaggtg gaactacccg tattgtaatt gtatttttca ccgggcgaat
     6601 attgcatcgg cagttcaatc aaatacttaa tccaatcact gcttgtcatc atcttatagt
     6661 ggggattctc cggattgtca tacggagtgc cgtattcgtc ccattcaaag cccgatgaca
     6721 tggtgagaaa atgttttaag gtaattcctt ttttttcgtc tgtccaagcg aggttattaa
     6781 acgatggcaa aaggttggag agcgtttcct gttcactgga gataaaccct ttatcaatag
     6841 tgatcccgat tattgccgac gcgaaacttt tggtaaccga atacatcggg tgaagatcat
     6901 tctgattcca gttattaaaa tactcttcaa gtaccagttt atcatttttc aagataagca
     6961 tactgtgaaa acctccatat acgccggcat caattgaatc catcaaaact tttagtgaca
     7021 ttaagtcaag accttcggtg attgggtcag aaatacttaa accatctcct aaatcagaag
     7081 gtttttcata tcgatatttc gtctggacct ggagatcttc tctttcacaa ctaataagaa
     7141 aagtcgcgat taaaagttga aagcaaggaa atatcaggtt tgtaaatttc atagcgcaat
     7201 aaaacaatac ctaaattatc aatatcatgc tttacaatca acattctttg ctaaaaatgc
     7261 ggtcataaat taatccttcc tgattcttta aaagatatta atcctgtttt gctaaatcac
     7321 tgataccttt agtattattg ctataaaagg taaatcaaat gtagtatgat aaaattaaac
     7381 ggtatttttc cgcccctgcc tacctctttt gatgcgcttg aaaatctcgc aactgataaa
     7441 ataaaagaga atattgtcaa tttaagccgg tacaatttgg ctggatttct tatactgggt
     7501 tccaatggag aactggtaaa tcttacagaa aaggaaatcc aggaagtata tgtggcatca
     7561 agggaagcga ttcgcgcgga taaaattatg ctggccggca ctggtgcaca atctacccgc
     7621 ggaacaataa cgaatactca agctgccgct gctgcaggtg ccgatgcggt attggtactg
     7681 aacccatcct actataaaag tctaatgacg acagatgcac tcgttgcgca ttatcatgca
     7741 gtcgcggatg cctcagcaat tcctgttcta atttataata tgccggcaaa ttccggcctg
     7801 gatatggatg cggaaacaat cttgaagatt tctgaacacg agaacattat cggactgaaa
     7861 gattccggag gcaatcttgc aaaaatggga aatgtaaaaa gaatggcaaa gcctgacttt
     7921 cagattttgg ccggctcagc aggtttttta ttgcctgcct ttactgttgg cgctgtaggt
     7981 ggaatcttag ctctcgctaa catcgcacct cagaagtgca ttagtattta tgaagattat
     8041 ttttcaggca atattgaaaa agcacaaaaa acgcagctgg aattaattcc ggtaaacgcc
     8101 gcggttaccg gcaaatgggg agtaccggct ttaaaaaccg ccatggatta tatggggtta
     8161 tttggaggca ccgcaaggaa acctttgctt ccccttaagg aagaaataaa aaaacaagta
     8221 acagacctga ttgaaaaaca cgttaagtag aatcaatgaa actattgaat tgtataatta
     8281 gtagccgata aacaggggtg agtgcaatac atcactctgt cgactcactc tttccttttt
     8341 cgaaattatt cctttctatc actgtatcat tggacactga aagtcaaaac ttttcaaaaa
     8401 atctccaaac catgtccaaa tttcaaagtt tccttcgtca attggatgta actaatgagt
     8461 aaacgttatg agcatcataa gacagattgg ttgtgaggca gcaaatgttt aaattttgaa
     8521 ataaattcta taactaaaac taaaatcaaa aaaaaatgaa aaagttaatt ctcttaatcc
     8581 cggcgatgtg gataatgagt ttcacaggta caggaccagg tattactaag gccgaacgta
     8641 aggctgccat cgaatccctc aacaaaacgc aggacgattt attggctacc attgaagagc
     8701 tttcggtgga acaattaaat tttaaaagca gccccgaatc atggtctgta tcggagtgcg
     8761 ttgagcacat cgcaatatca gaaacgaatc tgttcggtat gattcagggt acattaaaag
     8821 aacaggctga cgccgcttta aaaagtgaga ttaaacttac cgatgaacag gtatttgcag
     8881 ctattgtcga tcgtacatat aaggttaaga cacaagaagc attcgtgccc tccggaaaat
     8941 ttggttcaca tgaagctacg attaaggagt ttctcaataa acgtaaagca aatatcgatt
     9001 atcttaaaga tacccaggac gatcttcgta atcacttttt cacatttccg gtggaagccc
     9061 ttggtacggt agactcttat caactatttt attttatgag cggacatacc aaacgtcaca
     9121 cattacagat tgaggaggta atgagcgatc cttcctttcc caaaaaataa ttcatatttc
     9181 atcataaaac tttaaaaacg aaaaaatgaa agattattta ttattgtttc gtggaggcga
     9241 agctcatact gaaaggcaaa atcctgaaaa atggcaaacc cacatgcaaa aatggatgga
     9301 gtggatgaga ggacttcaag aacagggaaa acttgttgga gctcagccgc ttgatgaatc
     9361 aggtaaacaa ataaagggga caaagaaaat tgttaccgat gggccattca tggagggtaa
     9421 ggaaattgtt ggaggatatt tacaatgcaa ggcggactcg tatgaccagg cgcttgaaat
     9481 tgcaaaaggg tgccccatcc ttgaatttga aagcggcatt gtggaagtaa gggaaattca
     9541 ggaactgaag atgtgaggta ggattgacaa ccacaatcat acatttaagt tcctttatta
     9601 gaattctgta aaataagttt ggatgaataa ctaaatttaa acctttcaat ttgaagggtg
     9661 ccccaatccg atgtcaacat actgaaactt gctgaccatc tttttcgaaa tgaatcgaag
     9721 aagatggtcg ctgttttgac taaaatattt ggaacagaaa atctggaaac tgcagaagat
     9781 gtggttcagg acaccttgtt gcacgccatc aatgtttgga agatcaaagg cctgcccgac
     9841 aacccatccg cctggctttt tcgggtagct aaaaacaaag caattgacat tattcgcaga
     9901 aacaaacact cggtcaagta tgatttcagc atcggcgaaa cagcattgct tgcttcggaa
     9961 tataccctcc ataccgctat ggataatttt tggaaggaag aattgattga agacgatctg
    10021 ttaggaatga tgttcgtatg ctgtcatcct gaaatttctt cggaaaacca gatcacctta
    10081 atcctgaaaa ctctttgtgg atttagcact gcggaaatcg ctaaaacctt tcttacgaca
    10141 aatgatatcg tctcaaaacg gctttatcgc acgaaagaat ttttcagggt acacaaagta
    10201 gctttgagta ttccttctaa agaggaaatc aaaaagcgga ctgaggttgt actaaattcg
    10261 atctatcttc tttttaatga aggatacaac tccacacaca atgaatacct aatccgcaaa
    10321 gatgtgattg aagaggcgat ggcattatgc aaaatgcttt cagaaaacaa acacactcag
    10381 attccggagg tatttgcgtt gttggctctt atgtgctttc atgcagcgcg actcaatagt
    10441 cgtcttacaa gagatggaga aattattcta ttgtcagaac aggatcggag tacttgggat
    10501 gaagcattaa ttgagcaagg gaacgaattc atggataaag cagcctttgg caactcgatc
    10561 agcagctacc atctggaggc cgctattgcg tttgaacact gtactgcaaa gacgtttgag
    10621 agtacaaact ggaatcgcat tctggagtac tataactggc tctgcaccct atccccatca
    10681 ccaatcacag aactaaataa gggagtagcg attatgcagg ttcatggagc agaagcagca
    10741 ctcagggaat tgaatttgat aaaagaaaga aaaaaactgg agtcatacta tttgtaccat
    10801 agtttaattg gtgaaatcaa ttccaaaatg aacaacacca agcaggccat tgcaagtttt
    10861 aaattggcca tgcagctcac aaactccgaa cctgaaaaaa ggattctgca ttcaaaaatt
    10921 acagctttat taaattaggt tcgaaataat ctgatcctct aagttgacat tgtttgtgga
    10981 aaattgtccc ccttgtttta aggggactac agggggttta aatactattt aagaaccacc
    11041 cctaggaaaa ttattaacca cccaccttcg gtcgccaggc cccaccttcg gtcggcaggc
    11101 ctaccttaaa ataaggaggg gaaagtaccc ctgcctgtaa attgtccccc ttgttttaag
    11161 ggggactaca gggggtttga acaccatttt aagaaccacc ccaccttcgg tcggcaggcc
    11221 caaccttaaa ataaggaggg gaaagtaccc ctgcctggaa attgtccccc ttgttttaaa
    11281 gggactacag catagttaag caagtgaggc tttccacgcg gctcttttat tctaaatatt
    11341 tcgggaattg cattcatgaa ttttgtgaat tatgtttaca aaaaaatcta tatgtggaat
    11401 attaatacgc cgtggttcga tgtttcagtt ataacttgtc tggtagcact tggaagtatt
    11461 ttccttggcc attttgaaac agaaactccc aagcttcgaa aagttctaaa attgttggta
    11521 acagtggcca taatggttac tttatctgct acagtaggaa ggatttgggc ctttactttt
    11581 ttaggaatta tgatattagc tgtcctttac attcatatca tgtggctgcc ttccaaagga
    11641 attaacggat ggacggggga gcctcggaaa aaatattacg agctgcgggg atggaaatgg
    11701 aaagaatagg aaaatatttt tgcctatccc gggttagtct tgcattaaac acgattgaga
    11761 aaacagaatg ctggtgaata cttgttatga gaataaaaaa taaagactgt cagaataata
    11821 gcagttgtca aaccgtgttt gaaagatttt aatacaactt aatgcaagag ctaaataatc
    11881 caattggcat ggatttattt acaaactacg gaccggtttt gcgggtaaaa atgtcgatcc
    11941 aatcgaccac atagacgtca caaaatgtaa tcctaattca tttgggataa tatatttatc
    12001 acttcagaag tttgggtttg atgatttaaa tctaatactc taaaattgct tgtgcacgga
    12061 taatatttcc gcgccaactg aggggatggt atattttgat cattcaaata aatgtgatgc
    12121 gattagcaga tttgaaattt ggcctaaagt acaaagtcat tcaagacttt caacttgcgt
    12181 tgtctgacca gttcttttcc agcgatgagc ttgtcttcct gggttcatgc accatctttt
    12241 gagacaaatt tgatgtggat catcatgaac tccattttta tgatcaaagc cgtgaaatgg
    12301 atttgatatg caggtacgtt gatccggaac gcatagagga ggtgctggag acaaacttta
    12361 tagaagccgg cggttggaga catccaggga aaggtgagtc ccttcctgac acggtttcgc
    12421 caggagataa tgcaacagta caattcttta ttccatactt aaagtcactg gtggaaggtg
    12481 acccggcaac tgagaattgg tttttttggt taaagcggaa ttccaaattg cttgagttga
    12541 cgctatccaa aatgcaacac ttgcaattga aaatcagccc tatccgggaa gccaaagtga
    12601 tactggattt attgggtgtg acatatgatc caagtaagta ctttcagtgg ttggacaaga
    12661 aggtcaccta acgtaaaatt ggccgctccg gatttcctcc ccctcagaat cctttgagga
    12721 gaaacagcca gggtttttta tttcagtgat ccccgcctct ttgagtcggg caagcagctg
    12781 ggattttagt ctatttgatc agatgggtaa aaatctcaat taataccgca aatatcagcc
    12841 tggttattaa aatcataact tcgtattcaa attacttcaa ttaacaggct tacttattgc
    12901 catctttttt gaataaaatc ttgtgcatat ttgtgcatta tatgttcctt agttatacac
    12961 cattatggct acaagatttt agcgatgttg taccattcat gaatagttta attataagtt
    13021 tgaactttaa tttgtttgat ttcaactgtg atgttgtaaa ttgttagtgt aatcatacta
    13081 attgacagta tgaaacgatt taatctcata attcttctgt tattaattca agttctgcct
    13141 tcctgtcaaa atgatactgc gatactcata aaaaataacc ttgaatttac gagtggagtt
    13201 ctacttttaa gaatagttgg agacagcatt caaaatcatt atatcgtcaa tgccaatttc
    13261 atgaacaatt gggacttttc caatagacaa cggatcagca taaagtgcag ttctggcact
    13321 gatacaatca ttaccgttaa cttaatcaag cctaactatt atagactgga gttggtaagg
    13381 tcgataagag aaaaaattat tgggtggacc tatttctgaa acaagatgaa tatttggaaa
    13441 tccaaatcag agaaaaagat gagcctgaca ttgaatttgc cgggtcgtaa gctgctatta
    13501 ataattatct cttagaaaag gaaaatgctg cgaaagcaga taaacaagcc tactggatga
    13561 acttggtagt ttcgtaataa atatctgaca aatccatatc tttatatccc ccagaagcta
    13621 aataactgat ttgggcaaaa tacggggata gagagaaagt ggatgaaaaa agtcattttg
    13681 cttgtcctaa catcatcatt atccgccttt tcttttgggc aatcaaaatc attagaaact
    13741 ggtaagcaac tcccttactc tgacagaaaa ctgtctgtag gaattaacta tgcaatcggt
    13801 aacttctata gattgccgac catcagcagc actaaggatt tctcaggatt tgcatttggt
    13861 ggcattgaaa ttgagaagca tctcaaaaat taccttaccc tttatatcag gtcttcatta
    13921 ataatcaatt cttcaattcc aatccccctc ggagagtgtt tcgatgattg tgaatatgct
    13981 aatgtttggg aaaacgccgc gggtcttaga agaaatttta cagttgggaa aaaaagctta
    14041 attaatcttg gcttaggaat ctcattaaat cattatctct ggtctcataa ttactcaatc
    14101 caaagtacaa cagattttat taaggttaaa gatgaattta ataatactgg aataggtttt
    14161 gattcgaaca tcaattattt ttttagagta ggtgagggta acataaatca tttcggaatt
    14221 ggattcaatt attctccgac ttttattaat tcacagttgg gaaataaata cttctactca
    14281 atacatttca aactcattta taagcactcc ttcatcaaaa gaaatcgaaa agaataaggt
    14341 taaatacctc atctccctca attagcatat cattatttat gacctcttcc cttcgcaaac
    14401 cctatcattt aatgaatcca aatcccaaat ttgggaaact cttcgaaatt agtaaatcaa
    14461 gggtactttt tgtacttctg tgattcactg gtcgacaagg acatccaact tttgaggcag
    14521 atcttggcaa ccagagattc ttaaaggcag cgaagtcact ctacgttgtg ttctccacga
    14581 tcgcagaact acttaagcac gcgtagagat ttaactcaac tttctcatta gatttcaatg
    14641 aaaaccaaat ttagtaactc aattcatgaa agtatctgta atacgcaggg cagaaaattc
    14701 aggtaggaaa ttaaagagtg agtgtcagag tatgtgagtg agaaccttca ctctcactc
//
LOCUS       DASWHX010000107        19241 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_2687990, whole genome shotgun sequence.
ACCESSION   DASWHX010000107 DASWHX010000000
VERSION     DASWHX010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 19241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19241
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2687990"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..329)
                     /locus_tag="VGA21_07100"
     CDS             complement(<1..329)
                     /locus_tag="VGA21_07100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650308.1"
                     /translation="MPEIKLTFWTFLFLAAATQGIFLATILFSKKNKSQANYFLSTLI
                     ILFSITLIFYVFFWSGISSRFGLIFNFILKLVLLFGPLAYLYVKSVKGYALKPSDLKH
                     FYFFPLIT"
     gene            complement(378..2033)
                     /locus_tag="VGA21_07105"
     CDS             complement(378..2033)
                     /locus_tag="VGA21_07105"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX9650309.1"
                     /translation="MRNLTIVSLVTFIIVGCNSPSQKADLIITNAVIWTADELVPVAE
                     AIAISGDLILAVGSTEEILTYHGNQTEVIDAGGNFITPGFIDSHVHFLDGGFNLLSVQ
                     LRDAGTKVEFIKRIKEYAQTVEPGTWIMGGDWDHTNWGGELPSREWVDSVTTGHPLVI
                     NRLDGHMILANSAALEAAGVTKSVQDVDGGTIVRNIQGELTGIFKDNAMGMIYSAVPE
                     PSDLQKNAALSAAMNYVVSNGVTSVHNMGGRSGEHQVLKQARENNNLTVRIYDIFPLP
                     AWQSLLSEIENNGRGDNWLKIGGLKGFMDGSLGSHTAAFFEPFSDTPDDSGFLVNSEE
                     EMYEWISSADSAGLQVMVHAIGDKAINSLLNIYERVALENENLNRRFRIEHAQHISPP
                     DIPRFSKQNVIPGMQPYHAIDDGRWAKNVIGEERIKTTYAFKSLLENNARLAFGSDWF
                     VAPPTPLEGIYAAVTRRTLDEANPDGWVPEEKITVEEALIAYTINAAYAGFDEDIKGS
                     LSVGKLADLVMINANLLGIPAEEIRNASVVLTLVGGQIVYDNR"
     gene            complement(2046..2576)
                     /locus_tag="VGA21_07110"
     CDS             complement(2046..2576)
                     /locus_tag="VGA21_07110"
                     /inference="COORDINATES: protein motif:HMM:NF022588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HEX9650310.1"
                     /translation="MNKTVISSLILISYPILLLGQSEGLPRAYPLEMETLLYHETYLK
                     ITYTQALAATSKLENGMLWQLGANEPAEIMTTGDLLVGKDTLAAGAYSIFTIPNDSTW
                     TIIINKDVGLFGTYRYNEKMDLFRIEAAIIPDDSYNEHLKITFSRKGVPVTDLVIHWK
                     KIKLSIPVHLLEQKEN"
     gene            2654..3136
                     /locus_tag="VGA21_07115"
     CDS             2654..3136
                     /locus_tag="VGA21_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013453076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase family protein"
                     /protein_id="HEX9650311.1"
                     /translation="MSKPAFDDIFMELAVNLATRSHCIKRHVGAVLTKDTRIISIGYN
                     GPPSGTHNCDEEWPDTGCERDSKGSCSLAIHAEQNAILYAVKNNSVVSGSTLYVTLAP
                     CLSCARIIFTMGIEKVIYLKSYAEFKGIELDEGVEFLTKFGINVEKYHGQLSNVTHMI
                     "
     gene            complement(3145..4161)
                     /gene="murB"
                     /locus_tag="VGA21_07120"
     CDS             complement(3145..4161)
                     /gene="murB"
                     /locus_tag="VGA21_07120"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018478312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEX9650312.1"
                     /translation="MKITKNSSLKSLNTFGIDCIAEKLIRVEAKEDLQKLYDDKTLLV
                     PDQLILGGGSNVLFTKPTLAQVLLIEISGIKTLDSDSISTLVQSGAGEIWDHLVQNAI
                     AHKLGGVENLSIIPGKVGAAPIQNIGAYGEELANVFECLDAFDLSSGKFVKFNSKECN
                     FQYRNSFFKTEGKGKYIITDVTLKLLKNRQVNISYGAIKKTLEEMGSPPLNPVNVREA
                     VIRIRTSKLPDPSVIGNAGSFFKNPVVEKSHYESLKMNHSEIPAYNQDEARVKLPAAW
                     LIEKCGWKGKRVGNTGVHEKQALVLVNHGNATGKEVHELALEIQSSVLNEFGIILEAE
                     VNIV"
     gene            complement(4165..4611)
                     /locus_tag="VGA21_07125"
     CDS             complement(4165..4611)
                     /locus_tag="VGA21_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009580992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1572 family protein"
                     /protein_id="HEX9650313.1"
                     /translation="MVEHIKSLLLRDLTTLANEITQYGKESDLWIVKPGINNCAGNLC
                     LHLCGNLKHFIGAILGNSGYQRERDKEFSEKDVPKSDLLQNIEETKDSISLALSNLDD
                     SILAKEYPIEMFGGKITTGFFLVHLHGHLNYHLGQVNYHRRLLASS"
     gene            complement(4617..6311)
                     /locus_tag="VGA21_07130"
     CDS             complement(4617..6311)
                     /locus_tag="VGA21_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018479075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HEX9650314.1"
                     /translation="MTIEERIESWLNSPIDEDTRKSILHLKEQNGEEIADSFYKDLEF
                     GTGGMRGIMGVGPNRMNKYTIGMATQGLSNYLLRNFEGKKIKVAIAYDNRNNSQFFAE
                     TAANILSGNGIEVFLFENLRPTPELSFAVRNLQCQSGIVITASHNPREYNGYKVYWDD
                     GGQILPPHDSGIIAEIREITKFEKINFKGDSKLIHLLGEPMDKQYLEAIKPFTHSYPF
                     NKNLSIVFTPLHGTGATLIPGLLKELGFEKVKLVKEQAEPDGDFPTVVFPNPEEAEAL
                     SKAVELAKEINADLIMATDPDSDRVGIAVRDHEGEYHLINGNQTGILLLNYLIDKTKN
                     KENAFIAKTIVTTDLIDEIARQKGVRCYNTLTGFKYIAEVIRKKPNEEFIAGCEESYG
                     YMIGDFVRDKDAVSACAVIAELASAMKDKGKTLLDLLKDIYLEFGLYKERLFTLTRKG
                     KTGAGEIINMMNDFRNHPPDEINESKVVRSVDYKENKDVDMLTGEVNSTGQPVSDVLQ
                     FFTEDGSKISVRPSGTEPKIKFYFSVHRAVNQENFSEISHGLDNQLDDIIKSLKLK"
     gene            complement(6324..7418)
                     /gene="hppD"
                     /locus_tag="VGA21_07135"
     CDS             complement(6324..7418)
                     /gene="hppD"
                     /locus_tag="VGA21_07135"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007657880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HEX9650315.1"
                     /translation="METDFLPINGTDHIEFYVGNARQASLFYQNAFGFKQIAYMGPET
                     GNREKVSYVLEQGKVRLVLSAAANQDSPVTEHVKKHGDGVKVLALWVDDAGKSFHETV
                     NRGAKPAAPPQTISDEYGKAVIASIHTYGETIHTFVERKNYQGPFLPGYISRPSLLKV
                     VPIGLKYVDHCVGNVELGQMNRWVEFYEKVMGFSLLVTFDDKDISTEYSALMSKVVAN
                     GNSYIKFPINEPAKGKKKSQIEEYLDFYNGAGVQHIAIATDNIVDTVSEMRKRGVEFL
                     YVPERYYDDVLDRVGEIEEDLESLKEQNVLIDRDEEGYLLQIFTKPVQDRPTVFYEII
                     QRKGAKSFGKGNFKALFEAIEREQEVRGNL"
     gene            7609..7977
                     /locus_tag="VGA21_07140"
     CDS             7609..7977
                     /locus_tag="VGA21_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009582755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650316.1"
                     /translation="MKNKLAALNYADEAYDTIEEELHTLEDDMMEKHGPYLEEAINYV
                     HDEFCPDTEVLSPLAYLAKRYDSENGNFKVDLNEGVPVEIDDYPGKETRLVIVPGPPR
                     IILQIGNEGQEVVWKAAKGF"
     gene            complement(7974..8945)
                     /gene="trpS"
                     /locus_tag="VGA21_07145"
     CDS             complement(7974..8945)
                     /gene="trpS"
                     /locus_tag="VGA21_07145"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009186902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEX9650317.1"
                     /translation="MSRILTGIQSTGIPHLGNLLGAIIPAIELSRKENNDSFYFIADL
                     HSLTTIKDPEVLRQNTYSTAAAWLAFGFDTDRNTFYRQSHVPQVCELTWYLNCFTPFP
                     MLANAHSFKDKSERLADVNSGLFTYPVLMAADIILYDANFVPVGKDQQQHLEITRDIA
                     GAFNHKYGDTFIIPEALIDEKVMVIPGTDGQKMSKSYNNTISIFQSDEALRKNIMSIT
                     TDSLPLEEPKNPDKCNVFALYKLLAEPNDVEIMRKLYLNGNYGYGQAKQELYDLLINK
                     FEKERKAFNDLLTNPDEIEEQLHTGEVKAAGIADKVLNRVRKKLGYF"
     gene            9096..10166
                     /locus_tag="VGA21_07150"
     CDS             9096..10166
                     /locus_tag="VGA21_07150"
                     /inference="COORDINATES: protein motif:HMM:NF019242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HEX9650318.1"
                     /translation="MKKLSIITSIIFFSITGHAQINEITFKEMEHDFGSVKEEDGPVT
                     HEFTFINNTQAPLKVLTVRASCGCTTPGWTKEEVAPGKSGFVKAQYNPYNRPGPFNKT
                     LTVQTTSSQSIVLKIKGNVLPRLKTTTEQYPVVIGNLSFTTTSFNMEKVTPKTPVTRE
                     FEVLNSGTEPVEFIEAPNIPGHLKIMYLPRLVAAGETAKIVVIYDAKVKGDMGFLIDD
                     IKLFTNESSDGEKAVKVYATIEPYIPPMTREEKALAPVLSIDKSIVDFGTITQGEKVE
                     NEYLLKNSGKSTLEIVMTKPSCECTTVSLSKNSIEPGEEVSLKVVFDSQKIKGTQQKY
                     ITIFSNDPSGPSKRVTLRGVVN"
     gene            complement(10217..11437)
                     /locus_tag="VGA21_07155"
     CDS             complement(10217..11437)
                     /locus_tag="VGA21_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009582239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX9650319.1"
                     /translation="MSTNNTTETHKKQSGKQDLFESPDFYQLDDLLSEEHKLIRRSVR
                     DFVKKEISPNIEGWCERAEFPIQIVKKFGELGAFGPTIPQEYGGGGLDYISYGLIMQE
                     IERGDSGMRSTASVQGSLVMYPIYKFGNEEQRKKYLPKLATGEMLGCFGLTEPDFGSN
                     PAGMVSNFRDMGDHYLLNGAKMWISNSPKADIAVVWAKSEEDRIHGLIVERGMEGFST
                     PETHGKWSLRASTTGELVFDNVKVPKENLLPNKSGLGAPLMCLDSARYGIAWGSLGAA
                     MDAYFSARKYAMERIQFDKPIGSFQLVQKKLSEMLTEITKGQLLNWRLGKLMDEGKAT
                     TPQISLAKRNNVEVALKICREARQIHGAMGITGEYPIMRHMMNLESVVTYEGTHDIHL
                     LILGNEITGIPAFV"
     gene            11509..12477
                     /locus_tag="VGA21_07160"
     CDS             11509..12477
                     /locus_tag="VGA21_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013453327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase family protein"
                     /protein_id="HEX9650320.1"
                     /translation="MDKSELDPRVLEQIQKLEEKYEAMGQDISSYLDGLLHADYLTYW
                     DYIHIDTLLSLQNPKTSLKDEVIFIIYHQITELYFRMVLWETDQLVLDEDLTVETFKK
                     RLNRIINYFENLANSFDIMIDGMEKEQFLKFRMSLLPASGFQSAQYRLIEFACTDLIN
                     LVSPDKRNSSGQLPLREQYENIYWKQGATELATGKKTLTLKQFEEKYLDDFMEFISRY
                     ETKNINRIYLEKFAETDEGSISKILRKLDALININWCLSHYKSAVRYLQKDPEDIKAT
                     GGTNWQKYLPPRFQKIVFFPTLHNPAELKEWGKSWVKEEVFGQKGL"
     gene            12559..14391
                     /locus_tag="VGA21_07165"
     CDS             12559..14391
                     /locus_tag="VGA21_07165"
                     /inference="COORDINATES: protein motif:HMM:NF019284.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-binding protein"
                     /protein_id="HEX9650321.1"
                     /translation="MRRTVYILIITLFATLSVSFAQKLKLTNYYDTQNKIIKEVYQLQ
                     DSTLNGSYIGYFITGKVRVKGQYKRNKPVGIWEYFYENGNIKMRGELRNNSNYGPWEY
                     FHENGKKSMEGKILNGLRQGLWRFYYPSGKLTSDGIYINNFRSGEWNYYYEDGSKLGV
                     GNYKRGKGLYTEFYPNGNKFMEGIKDNGRSQGNWSIYFEDGKLQALGDYRDGLKEGVW
                     QYFYKNGLVQSEGIYKDDMQSGDWTYYGPSGNMSSKGKLVEGMRDGYWVIFDEKGGLK
                     GEGDFAKGNGFYRELYPDGNIKIEGNIVNELKNGKWSFYYPTGELEGDCIYQNGQGKY
                     VGYYMNGVKKMSGQMDDDIMIGIWELFEPDGSLAGYYRPYYDKDGPIFELETAVAKPK
                     GVSEYKDRRISEFRFKRKKFTPFKPKLNEFKGLIFAYNPFAVFLGDLPVGLEYYVQER
                     LGHEVLFSLKRDPFFKPETDIGLNEDYSRGYAISLRQKYYFPHTKIGILYIGQELRYT
                     ATRHKATILDNNDPPSPQALKIEEKKYEYSILLGTRLVKDLNSKGITADIFVGIGTGF
                     RDYSQDFPSNTATDEVFKDVKLGSVPLSFRLGINFGYIFGLKGK"
     gene            14388..14750
                     /locus_tag="VGA21_07170"
     CDS             14388..14750
                     /locus_tag="VGA21_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019946808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEX9650322.1"
                     /translation="MKTVVVGASPDAFRYSYKAIKSLLDHHNQVIALGIREGAVFGQP
                     ILDIRERPIIKEVDTLTIYVSAPNLADYEDYLLSLNPGRIIFNPGAENATLQKKANHL
                     GIKTINDCTLVMLSVGAY"
     gene            complement(14744..16078)
                     /locus_tag="VGA21_07175"
     CDS             complement(14744..16078)
                     /locus_tag="VGA21_07175"
                     /inference="COORDINATES: protein motif:HMM:NF025224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650323.1"
                     /translation="MFFQLKPSPLFACFLVFFSSKIWAQEIKIPEGTKPGDTITIIEK
                     EPSPLPAINRLDYDSLRKARELMSRQLELVKFESFNANTRKDTLEYLSLAYLNMNEIP
                     KSVKECKALKTIKLSYNQLSRIPRYFRKFPELKTLQIAGNNSGNRSIKIPRLKNVEII
                     DLQENGLSKLPLSIRKIKSLREINLSRNFFTRIPLRLTRNKNLVTLKLNENRLKPDEQ
                     KLHKLAQIEYLRMNKCDINYFTDNLFDLVNLKELQLAENNIRVLPDRISELSRLESIS
                     LYKNSIDSLPPGFYSLKNLKIADLYYNKIERISSDIENLKNLEILYLSFNSIFLVPEN
                     IGALASLKQLYLHHNRLFYIPESIGNLNELNILHLQNNLLNYFPPQILKLKKLRELDI
                     SYNDIEEFPENLADLKNMKLLFLQKNPFWNKVDNEITFIEQFRRMGTNVIFQ"
     gene            16175..18130
                     /gene="gyrB"
                     /locus_tag="VGA21_07180"
     CDS             16175..18130
                     /gene="gyrB"
                     /locus_tag="VGA21_07180"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017732224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEX9650324.1"
                     /translation="MSDEKTVAKSSYSAQNIQVLEGLEAVRKRPAMYIGDVGVKGLHH
                     LIWEVVDNSIDEALGGYCTDIEVQIMEDNSIIVIDNGRGIPTDMHIKEKRSALEVVMT
                     VLHAGGKFDKDSYKVSGGLHGVGVSCVNALSESLKATIHRNGTIYEQEYAKGFPKYPV
                     REAGTTGKTGTIIHFKPDVGVFQATEFNYETIASRLRELAFLNAGIKLKLTDFRTKDN
                     GNYKSDDFLSEGGLTEFVKYLDTSKEKLIPNPIFIDTIKGDVPVQVALGYNTSFTETV
                     VSYVNNINTIEGGTHVAGFRRALTRTLKSYADKSGLLDKAKVEISGDDFREGLTAVIS
                     IKVAEPQFEGQTKTKLGNSEVMGAVDTAVGETLQYFLEENPKEAKQIVSKVILAAQAR
                     HAARKAREMVQRKNVLGGTGLPGKLADCANNDPAVSEIYLVEGDSAGGSAKQGRNRNF
                     QAILPLRGKILNVEKAQEHKIYENEEIKNMITALGVSFGTDEDDKAVNTAKLRYHKII
                     IMTDADVDGSHIRTLILTFFFRYLRELIEKGYLFIALPPLYQIRKGKEERYCWTEEER
                     ERVVKELAKDGKTDSVGIQRYKGLGEMNPEQLWSTTMDPERRSLKKVSIESAAEAEHL
                     FSMLMGDDVAPRREFIEKNARYANIDI"
     gene            complement(18132..19022)
                     /locus_tag="VGA21_07185"
     CDS             complement(18132..19022)
                     /locus_tag="VGA21_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018474361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HEX9650325.1"
                     /translation="MTKIAAIDIGSNAVRFQVDRILTHNGNTEFKRLEYIRFPLRLGH
                     DVFTTGRISEDIKEKFLKLLHSFKLLIDLYEVSVVKACATSAMREAENGQHIAKLAEQ
                     AYDFPIEIIDGAQEAILINKAIKPYIDESMYLHIDVGGGSTELSILKNRERILSESFM
                     IGSVRSLKQVYSASIWETMKRWVKVNVHRKYPGIKGIGTGGNINKIYNLTGKKLSKKI
                     SVNTINATINEIGRYSEEDRINILKLNPDRADVIIPAAKIYLSVMEYAGINTIMVPEV
                     GLKDGLIYSIYEDLTRQKVL"
     gene            complement(19076..19225)
                     /locus_tag="VGA21_07190"
     CDS             complement(19076..19225)
                     /locus_tag="VGA21_07190"
                     /inference="COORDINATES: protein motif:HMM:NF023179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HEX9650326.1"
                     /translation="MNENFHLGYAYDKTTSALRQYSSGTHELMINYRIRIPKLHKGLE
                     CPSYF"
BASE COUNT         5750 a         3830 c         3897 g         5764 t
ORIGIN      
        1 gttataagcg gaaaaaaata gaagtgtttc aggtcagatg gtttaagcgc gtatcccttt
       61 acagatttaa catacaagta ggcgagtggg ccaaaaagga gtactaattt tagaatgaaa
      121 ttaaatatga gtccaaaccg gctggagatc cctgaccaga agaagacata aaaaataagc
      181 gtaatggaaa acaatatgat caaagtcgac aagaaataat tcgcctgact tttatttttc
      241 ttactgaaga gtatggtggc aagaaaaatt ccctgcgtgg cggcagccag gaacaaaaat
      301 gtccaaaaag ttagtttgat ttcaggcatt tgaacgggtt aacgccgggc aaatccacaa
      361 aaactaaacc acattcgtta gcgattatcg taaacaattt gcccgccgac taaggtaagt
      421 acgacagaag cgtttcggat ttcttccgca ggaatgccga gcaaattggc gttaatcatt
      481 acaaggtcgg cgagttttcc cacactaaga cttcccttga tatcttcatc gaaaccagca
      541 tatgcggcat taatggtata tgcaattaat gcttcctcaa ctgttatttt ttcttccgga
      601 acccacccgt ccgggttcgc ttcatccagg gttctcctgg ttactgcagc atagattcct
      661 tccagcggag tagggggagc aacgaaccaa tcactgccga aagccagcct ggcattattt
      721 tcaagaagtg acttaaacgc ataggttgtt ttgattcttt cttcaccgat tacatttttt
      781 gcccatctac catcgtcaat ggcatgatag ggttgcatgc cgggaattac attttgttta
      841 ctgaaccttg gtatatctgg tggtgagatg tgctgagcat gttcaattcg aaatctccgg
      901 ttaaggtttt cattctccag ggcaacccgc tcataaatat ttagcaggga gttaattgct
      961 ttgtcaccaa ttgcatgaac catcacctgc aggcctgctg aatcagcgct agaaatccat
     1021 tcatacatct cctcttcaga gttaaccagg aagccggagt cgtcaggtgt atcactaaaa
     1081 ggctcaaaga aagctgcagt atgagacccg agtgatccat ccatgaaacc tttaagtcca
     1141 ccgattttca accaattatc accacggcca ttattttcaa tttcactcaa taaggattgc
     1201 catgccggta gtggaaaaat atcataaatt cttacagtta aattgttatt ctctcttgcc
     1261 tgcttcagta cctgatgttc gcccgacctt ccgcccatat tatggacact tgtgactcca
     1321 ttggaaacaa cgtaattcat tgcagcagaa agagcggcat tcttttgcaa atctgaaggc
     1381 tcaggtacgg cactataaat catacccatc gcgttatctt tgaatattcc agttagctca
     1441 ccttggatgt ttcttacgat tgttcctcca tccacatcct ggaccgattt tgtaactccc
     1501 gcggcttcga gagccgccga gtttgccaga atcatatgtc cgtctaacct gttaatgacc
     1561 agcgggtgac cggtcgttac ggaatctacc cattcgcggc tgggaagttc accaccccaa
     1621 ttcgtatgat cccaatcacc acccataatc caggtaccgg gctcaacagt ttgcgcatat
     1681 tcctttattc tttttataaa ttcaactttt gttccggcat ctcgcaattg tactgataat
     1741 aaattgaagc caccatccaa aaaatgcaca tgactatcaa taaagccggg ggtaataaaa
     1801 tttccgccgg catcaattac ttctgtttga tttccatgat aggttaatat ttcttcagtc
     1861 gatccaacgg cgagaatcaa gtctccgctg attgcaatgg cttccgccac aggaaccaat
     1921 tcatcggctg tccatataac agcgttcgtt atgattaggt cagctttttg agaaggtgaa
     1981 ttgcagccaa caataataaa tgttacaagg gagacaatag ttagatttct cattgtgtga
     2041 tttcattaat tttctttctg ttccaggaga tgcaccggaa ttgataattt aatttttttc
     2101 caatggataa ccaggtcagt aacaggaact cctttacgtg aaaaggtaat cttaagatgt
     2161 tcattgtagg aatcgtccgg aattattgct gcttcaattc tgaaaagatc cattttttca
     2221 ttgtaccggt aggtaccgaa taggccaacg tctttattaa tgataattgt ccaggtggaa
     2281 tcatttggta tagtgaaaat ggaataagcg ccggcagcaa gggtatcttt tccaaccagt
     2341 aaatctccgg ttgtcataat ttctgccggc tcattggcac ctaactgcca gagcattcca
     2401 ttctccagtt tactggttgc ggcaagagcc tgggtgtaag taatcttgag gtatgtctca
     2461 tgataaagca aagtttccat ttccaacgga taggctcttg gaagaccttc tgattgtcct
     2521 aaaagaagaa ttggatagga aatcaggatt agactagaga taactgtttt attcattaag
     2581 ttaattactt tcgaatcgtt aattttggct tacaatatat ataaacgatt aaagaaagcg
     2641 aaggtaatta cagatgagta aacctgcatt tgatgatatt ttcatggaac tggctgtaaa
     2701 tctggctacc aggtcgcatt gcattaaaag gcatgtgggc gcggttctga caaaagacac
     2761 caggataatc tcaatcggat ataatggacc gccttccggt acccataatt gtgatgaaga
     2821 atggccggac accggttgtg aaagagattc aaagggaagc tgttccctgg ctattcatgc
     2881 cgaacagaat gctatccttt atgctgtgaa aaataattct gtagtttccg gctcaacttt
     2941 gtatgtaacg cttgcaccct gcctttcctg tgcccggata atttttacca tgggaattga
     3001 gaaagttata tacttgaaat cttatgctga atttaaggga atcgagttgg atgaaggtgt
     3061 tgaatttctg actaaatttg gcattaatgt agaaaaatac cacgggcaac taagtaatgt
     3121 tacccatatg atctaacata taatctaaac tatatttact tccgcttcca ggataatccc
     3181 aaactcattg agaacagaac tttgaatttc cagggctaat tcatgcactt ccttgccggt
     3241 agcgtttcca tggttaacta agaccagcgc ctgtttttca tgcactcccg tattgccaac
     3301 tcgttttcct ttccagccac acttttctat aagccaggca gcaggaagtt tcaccctggc
     3361 ttcatcctga ttgtaggcgg gaatttcgga atgattcatt ttaagacttt cataatgaga
     3421 cttttcaaca accgggttct taaaaaaact tccggcatta ccaattactg atggatctgg
     3481 taattttgat gtccgaattc tgataacggc ttctcttacg ttgaccggat ttaacggtgg
     3541 acttcccatt tcttcaagag ttttcttaat tgcaccgtac gaaatattca cctggcggtt
     3601 cttcaacagc tttaaggtaa catccgtaat aatatatttt ccctttcctt ctgttttaaa
     3661 aaaactgttt ctgtactgaa agttacattc tttggaattg aatttaacaa atttaccaga
     3721 gctgagatca aaggcatcca ggcattcaaa aacatttgcc aactcttcgc cgtaggctcc
     3781 aatattttga atgggagcag ctccaacttt tcccggaata atactgagat tttctacccc
     3841 tcccaattta tgagcaattg cattttgaac caggtggtcc caaatttctc cggcgccgga
     3901 ctggacaaga gttgaaatac tatctgagtc aagcgtttta atgccggaga tttcaattag
     3961 cagtacctga gcaagcgtag gttttgtaaa aagaacatta cttccgcctc caagtattaa
     4021 ctggtcagga acaagtaatg ttttatcatc atataacttt tgtaggtctt ccttggcctc
     4081 aactctaatt aatttctctg caatgcaatc aattccaaaa gtatttaacg actttaaaga
     4141 tgagtttttg gtaattttca ttccttagct ggatgccagc agtcggcggt ggtaatttac
     4201 ctggccaagg tggtaattca aatggccgtg caggtgaacc agaaagaatc cggttgtaat
     4261 tttgcctcca aacatttcta ttgggtattc ttttgccagg attgaatcat caagattaga
     4321 taaggccaat gagattgaat ctttagtttc ctcaatattt tgcaacaagt cagactttgg
     4381 cacatctttt tcactgaatt ctttatcgcg ttccctttgg taaccggaat ttcctaagat
     4441 agccccgata aaatgtttca aatttccgca aagatgaagg cataagttcc cagcacaatt
     4501 attgattcct ggtttaacaa tccataaatc tgattctttc ccgtactgtg taatctcatt
     4561 agctaaagtt gtaagatcac gaagcaataa tgattttata tgttcaacca tgcgtattat
     4621 ttaagtttaa ggctctttat aatgtcatcc aattggttat caagaccgtg gctaatttca
     4681 gaaaaatttt cctggtttac agcccggtgc acactaaaat agaattttat tttaggttct
     4741 gttcctgatg gacgcactga tattttactg ccatcttccg taaaaaactg gagaacatct
     4801 gaaacgggct gcccggttga atttacttca cctgtaagca tatcaacgtc tttattttct
     4861 ttgtaatcta cacttcggac aaccttagat tcgtttattt cgtccggcgg atggtttcga
     4921 aaatcattca tcatgtttat gatttccccg gcgcctgttt tacctttcct ggtaagagta
     4981 aataatcttt ctttataaag gccgaattca aggtagatat cttttaataa atccaacaga
     5041 gttttaccct tatccttcat tgcagatgcg agttcggcaa taacggcgca agcagaaaca
     5101 gcgtctttat cccttacaaa atctccaatc atatatccgt aactttcttc acaaccggcg
     5161 ataaattctt cattgggttt tttcctgatc acttcggcta tgtatttgaa tccggttaaa
     5221 gtattgtagc accggacccc cttttgtctc gcaatttcat ctatcaaatc tgttgttact
     5281 attgttttcg caatgaaagc attttcctta ttttttgttt tatctattaa gtaattaaga
     5341 agcaatattc cggtctgatt tccatttatt aaatgatatt ctccttcgtg atcacgcaca
     5401 gctattccca cccggtcaga atccggatct gtagccataa ttaggtcagc atttatttct
     5461 ttggctaact caactgcttt agataaagct tccgcttcct cgggattggg aaacaccacc
     5521 gtcggaaaat caccgtccgg ttctgcctgc tctttaacaa gttttacttt ttcaaagcct
     5581 aattctttga gaaggccagg gatgagggta gctcccgttc catgcaatgg agtaaaaaca
     5641 atagaaaggt ttttgttgaa tggataggaa tgggtaaatg gttttatagc ttccagatat
     5701 tgtttatcca ttggttctcc cagaaggtga atcagtttgg aatcaccctt aaaattaatt
     5761 ttttcaaact tggttatttc tcttatttca gcaatgatac ctgaatcatg gggaggtagt
     5821 atttgtccgc cgtcatccca gtacacttta tatccattat attccctggg attgtgagaa
     5881 gcggtaatta caatcccgct ttggcattgc aagttcctga ccgcgaaaga taattcgggg
     5941 gtgggacgca aattttcaaa cagaaatact tcaattccgt ttccggaaag aatattcgcg
     6001 gcagtttctg caaaaaattg gctattgttc ctattgtcgt acgcaatggc aaccttgatc
     6061 tttttacctt caaaattccg gagtagataa ttggataatc cctgcgttgc catacctatt
     6121 gtatacttgt tcatgcgatt gggaccaact cccattattc cccgcatacc tccggtgcca
     6181 aattccaaat ctttataaaa ggaatctgca atctcttctc cattttgctc tttaagatgc
     6241 aaaatagatt tccgtgtatc ctcgtctatt ggtgaattca accaggattc aattcgttct
     6301 tcaattgtca ttgatttttt tccttagaga ttgcctctta cttcctgctc cctttcaata
     6361 gcctcgaaca atgctttgaa atttccctta ccaaatgatt tcgcgccttt cctctgaatt
     6421 atttcataga aaactgtagg tctgtcctga actggtttgg taaagatttg caataagtag
     6481 ccttcttcat cacgatctat taaaacattc tgttctttca atgattccaa atcttcttca
     6541 atctcaccaa ctctatccag aacgtcatca taatatcgct ccggcacata gaggaattca
     6601 acccctctct tgcgcatttc gctaacggtg tcaacaatat tatcagttgc aattgcaata
     6661 tgttgaacac ccgcgccatt gtagaagtcg aggtattctt caatctgaga tttcttctta
     6721 ccttttgccg gctcattgat tggaaatttt atatagctat ttccatttgc caccaccttt
     6781 gacatcagcg ctgaatattc tgtggagata tccttgtcat cgaatgttac cagcaagctg
     6841 aagcccatta ccttttcata aaactcaacc cacctgttca tttgtccaag ttctacatta
     6901 ccgacacagt ggtcaacata ttttaacccg ataggaacaa ctttaagtaa agaaggtctg
     6961 ctgatatacc ccggtaaaaa cggaccctgg tagttctttc tttcaacgaa agtgtgaatt
     7021 gtttctccat aagtgtggat actcgcgatt accgctttac catattcatc agaaatagtt
     7081 tgaggtgggg cagccggttt ggctccacga ttaacagttt catgaaatga tttcccggca
     7141 tcatcaaccc acaaagccaa tacttttaca ccatcgccat gctttttaac gtgctctgta
     7201 acaggggagt cctggttggc tgccgcggaa agaacgagtc ttactttccc ttgttccaga
     7261 acataagaga ctttttccct gtttcctgtt tccggaccca tatacgcaat ttgtttaaag
     7321 ccaaatgcgt tttgatagaa tagggaagcc tggcgagcat ttccaacata gaattctata
     7381 tgatcagtgc cgtttatggg taaaaaatcc gtttccatgg caaatgtgtt tgagattttg
     7441 aaattgttta gtaaatctag cgctttggtt aattttttga aacttttgat gtttcagatt
     7501 ttattttaaa aatccttggg aagtttaact tccggtatta aattttgtga atcaatataa
     7561 gatatggata tagactctct agaccggtca ttacgacaaa tagctgaaat gaaaaacaag
     7621 ttggcagccc tgaattacgc tgatgaagct tacgatacta ttgaagaaga attgcatact
     7681 cttgaagatg acatgatgga aaaacatggc ccttatcttg aagaggcaat taattatgtt
     7741 cacgatgaat tttgccccga tacagaggta ctttctccgt tagcttacct ggccaagagg
     7801 tatgattcgg aaaatggcaa ttttaaagtg gacctcaatg agggcgttcc ggtggagata
     7861 gatgattacc ccggcaaaga aactcgattg gtgattgttc ctggtccgcc ccgcattatt
     7921 cttcaaatag gaaatgaagg ccaggaggtg gtttggaaag ctgcaaaggg attttaaaaa
     7981 taaccgagtt tttttcttac cctgtttagc actttatctg caatccccgc tgccttaact
     8041 tcgccggtgt gcaactgttc ttcaatttca tctgggttag ttaacagatc gttgaatgcc
     8101 tttctttcct tttcaaactt gtttataaga agatcgtata attcctgctt agcctgtcca
     8161 taaccataat taccattcag gtagagtttt cgcattatct ctacatcatt cggctccgcc
     8221 aacaatttat agagcgcaaa tacattgcat ttgtccgggt tctttggttc ttccagcggt
     8281 aagctatcag tagttataga cattatattt tttcgaagag cttcatcaga ctgaaaaata
     8341 ctaatggtgt tattatagga tttgctcatc ttctgcccat ctgttcctgg aatcaccatc
     8401 accttctcat caatcagagc ttcaggaata atgaaagtat ctccatattt gtgattaaag
     8461 gcaccggcaa tatcccgcgt aatttcaaga tgctgctgct gatctttacc caccggcaca
     8521 aagttggcat cgtaaagaat gatgtcggct gccattagca ccggataagt aaataacccg
     8581 gagttgacat ctgccagtct ttctgattta tctttgaagc tgtgcgcgtt agccagcata
     8641 ggaaagggtg taaaacaatt cagataccag gtaagttcgc acacttgtgg cacgtgagat
     8701 tgccggtaaa aagtattcct gtccgtatca aaaccaaaag ccagccaggc ggcagcggtg
     8761 gagtaggtat tttggcgtag aacttcggga tccttgattg tggttaggga atgcaaatcg
     8821 gcgataaagt aaaagctgtc attattctct ttcctggata attcaattgc gggtatgatg
     8881 gcacccagca gatttcccag atgtggaata cctgtacttt gaatgccggt taagattctg
     8941 gacataagtt gtaatattgt ttgttaatat aatgttataa gagagacaat tctttgataa
     9001 aaatgaaaaa aacccaaatt ttgtaacggt tacaaaacta ggtaaaacca cataaatcag
     9061 agggaaaaca gagttaaagg ataaatacaa aaaaaatgaa gaaattatca ataattacaa
     9121 gcattatctt cttttcaata acagggcatg ctcagattaa tgaaataact tttaaggaaa
     9181 tggagcatga ttttgggtcg gtaaaggaag aagatggtcc ggttacgcat gaattcacct
     9241 tcatcaataa tacccaggcg cctctcaaag tattaactgt gagagccagc tgtggctgta
     9301 ctaccccagg gtggacaaag gaagaggtag ctcccggaaa atcaggcttt gtaaaagcac
     9361 aatataatcc ttacaatcgc ccgggtccat tcaacaagac cttaacagtg cagactactt
     9421 ccagtcaaag cattgtacta aaaattaaag gaaatgtact tccccgacta aaaaccacta
     9481 cggagcaata tcctgtggtt atcggcaatc tcagtttcac gaccacttca ttcaatatgg
     9541 aaaaagttac gcccaaaacg ccggtgacgc gtgagtttga agtgttaaat agtggaactg
     9601 agcccgttga atttatagaa gcaccaaata ttcccggtca tctgaagatc atgtatttac
     9661 ccaggctggt tgcagccgga gaaaccgcga aaattgtagt catctatgat gctaaagtga
     9721 aaggtgatat gggtttttta attgatgata taaaactttt tacaaatgaa tccagtgatg
     9781 gcgaaaaggc agtgaaagtt tatgcaacaa ttgaacccta tattcctcca atgaccagag
     9841 aagaaaaggc tttagctcca gtgctttcga ttgataagtc aatagttgat tttggaacta
     9901 tcacccaggg agaaaaagtg gaaaatgaat acttattgaa gaattccgga aaaagcacac
     9961 tcgaaattgt gatgaccaaa ccaagctgcg agtgtacaac agtttcccta tcaaagaata
    10021 gtatcgaacc aggcgaggag gtttctttga aagtagtttt cgattcacaa aaaattaaag
    10081 gaactcagca gaaatatatt accatctttt ccaacgatcc ctcaggaccc tcaaagagag
    10141 ttacattacg tggagtagtc aattaaaagc aggaatacct gaccttcttc ctcgttgcaa
    10201 gcagagagga gttttattaa acgaaagccg gtattcctgt aatctcattt ccaagaatca
    10261 gaaggtggat atcatgtgtg ccttcgtagg taacaactga ttcaagattc atcatatgcc
    10321 gcattattgg atattcaccg gttattccca ttgcgccatg aatttgtctc gcttcccggc
    10381 agatttttag tgcaacttct acattattcc tttttgccag ggaaatctga ggcgtggtag
    10441 ctttaccctc atccattagt ttacccagcc gccagtttaa tagctgtcct ttggttatct
    10501 ctgtcagcat ttctgaaagt ttcttttgta ccaattgaaa cgagccaatg ggtttgtcaa
    10561 actggattct ttccatggca tatttacgcg cggaaaaata tgcatccatt gcagccccca
    10621 gcgatcccca ggcaattccg tatctcgctg aatccagaca catcaaagga gcgcccaatc
    10681 cgcttttgtt tggcagcaaa ttttccttag gtacttttac gttatcgaaa accaactctc
    10741 cggtagtact tgccctgagc gaccatttcc catgcgtttc aggagtggaa aaaccttcca
    10801 ttccgcgttc aactattaaa ccatgaattc gatcctcttc acttttagcc cacaccacag
    10861 caatatccgc tttaggagaa tttgatatcc acattttggc tccgttcagc aggtaatggt
    10921 ctcccatatc cctgaagttc gaaaccatcc ccgccggatt acttccaaaa tcaggttcag
    10981 tcaatccaaa gcagcccaac atttcacccg tggcaagctt gggcaggtac tttttccttt
    11041 gttcttcatt gccaaactta taaatcggat acataaccag ggaaccctga accgatgcag
    11101 ttgatcgcat ccctgagtcg cctctttcaa tttcctgcat aatcaggcca taggatatat
    11161 aatcaagacc gccaccaccg tattcctgcg gtattgttgg tccaaaagcc cctaattcac
    11221 caaatttctt aacgatctga atgggaaatt cggccctttc acaccatcct tcaatattcg
    11281 ggctgatttc cttcttcaca aaatccctga ctgacctgcg aatcaattta tgctcttcac
    11341 tcagcagatc atccagctgg taaaaatccg gagattcaaa caaatcctgc ttgccggatt
    11401 gctttttatg ggtttcagtg gtattatttg tgctcattcg gttgctcttt tgttaactcg
    11461 tatttaagta tcaaatttac tataaattga aaaccaatct ctacatctat ggataaatcg
    11521 gaactcgatc ccagggtact ggaacaaata caaaagctgg aagaaaaata cgaagccatg
    11581 ggacaggaca tctcctccta cctggatgga ttacttcacg ctgattattt aacttactgg
    11641 gattacatcc acatagatac tttgctaagt cttcaaaatc ctaaaacaag cctaaaggat
    11701 gaagttattt ttataattta tcaccagata acggagcttt acttcagaat ggttctatgg
    11761 gaaacagacc agctggtttt ggatgaagat ttgaccgtcg aaacatttaa aaagcgattg
    11821 aatagaataa tcaattactt tgaaaacctg gcaaattcct ttgacataat gattgatggg
    11881 atggagaagg aacaattcct gaaattcaga atgtcattac tccctgccag cggatttcaa
    11941 tcagctcaat acaggctcat tgaatttgcc tgtaccgatt taatcaacct tgtaagtccg
    12001 gataaaagaa actcgtccgg acaactacct ttgcgagagc aatatgaaaa tatatactgg
    12061 aaacaaggtg caacggaact ggccaccggt aaaaaaactc tcaccttaaa acagtttgaa
    12121 gaaaaatacc tggatgattt catggaattc atttccaggt atgaaacaaa aaacattaac
    12181 cggatttacc tggaaaagtt tgctgaaacc gatgagggca gcatttcaaa aattctaaga
    12241 aaactggatg ctctgataaa tattaattgg tgtctttctc attacaaatc ggcagttcgt
    12301 tatctccaaa aagatcctga agacattaaa gcaaccggag gtacaaactg gcaaaaatat
    12361 ctgccaccca ggtttcagaa aattgtattc tttccaaccc ttcataatcc tgccgaacta
    12421 aaggaatggg gcaaatcctg ggtaaaagag gaggtttttg gccaaaaggg gctataaaat
    12481 caattctgac atttggaagt tgtctttaaa ctggattccg gtattataaa ttcataaatt
    12541 tggataaata aattcgaggt gaggcgaact gtttacatac ttatcattac attgtttgca
    12601 actctctccg tgtcattcgc acagaagtta aaacttacca attactacga cactcaaaat
    12661 aagataatta aggaagttta ccagcttcag gactccaccc tgaacggcag ctatatcggt
    12721 tactttatta cgggaaaggt aagggtgaaa ggacaataca agcgcaataa acctgtgggt
    12781 atttgggagt atttctatga aaatggaaat attaaaatgc ggggtgagtt aaggaataac
    12841 agcaattacg gaccatggga atattttcac gagaatggaa agaaaagtat ggaaggcaaa
    12901 atcctgaatg gattgcggca aggcttatgg aggttttatt atccatcggg aaaattgacg
    12961 tcggatggta tttacattaa taatttcaga tcgggtgaat ggaattatta ttatgaagat
    13021 ggctccaaac ttggagtggg taattataaa cgcggcaaag gactctacac tgaattctat
    13081 ccaaatggaa ataagttcat ggagggaatc aaggataatg gccggagcca gggcaattgg
    13141 tcgatttatt ttgaggatgg taaattgcag gcgctgggcg attaccggga tgggcttaaa
    13201 gagggggtat ggcaatattt ctataagaac ggattagttc agtctgaagg aatctataag
    13261 gatgatatgc aaagtggcga ttggacctat tacggcccat cagggaacat gagttcgaaa
    13321 ggaaaactcg tggaaggcat gagagatggg tactgggtga tttttgatga gaaaggagga
    13381 ctaaaaggag agggagattt tgcaaagggt aacggattct atagagaatt ataccccgat
    13441 ggaaatatca aaattgaagg caatattgtt aatgaactaa aaaatgggaa atggagtttt
    13501 tactatccta ccggcgagct ggaaggtgat tgtatctacc aaaacgggca gggaaagtac
    13561 gtaggttatt acatgaatgg agttaaaaaa atgtcaggtc agatggatga tgacatcatg
    13621 atcggaattt gggaattgtt tgaaccggat ggatctctcg ccggatatta cagaccttat
    13681 tatgataagg acggtccaat ctttgagctc gaaactgccg tagcaaaacc aaaaggggta
    13741 tcggaatata aagaccggag aatttcagag tttagattca aaaggaaaaa atttactccc
    13801 tttaaaccca agttaaacga attcaaaggg ttgatatttg cctacaatcc atttgcagtt
    13861 tttctgggtg atttgccggt aggtcttgaa tattatgtac aggaaagatt aggacatgaa
    13921 gttcttttta gcctgaagag agatcccttt tttaagcccg aaactgatat cggactgaat
    13981 gaagattata gccggggata tgccattagt ctgaggcaaa aatattattt cccgcataca
    14041 aaaattggca ttttgtatat tggtcaggaa ttaaggtaca cggcaacccg gcacaaggca
    14101 acaattttgg ataataatga tcccccttcc ccccaggctc ttaaaatcga ggaaaaaaaa
    14161 tacgaatact caattctttt aggaacccgt ttggttaaag atttgaattc aaaaggaata
    14221 accgcggata tttttgtagg cattggaact ggatttagag actattccca ggattttcca
    14281 tccaacaccg caacagatga ggtttttaaa gatgtgaaac ttggttcggt gccactctca
    14341 ttcagactgg gaattaattt cggctacatt tttgggttga agggtaaatg aaaacagttg
    14401 tagttggagc cagccccgat gctttcaggt attcctacaa agcaataaaa agtttattag
    14461 atcaccataa tcaggttatt gccctgggaa tccgggaagg tgcggtgttt ggccagccca
    14521 tccttgatat cagggaaaga ccgataataa aggaagtaga tacattgacg atttatgttt
    14581 ctgctcctaa cctcgcggat tacgaagatt atttgctttc actaaatcca gggaggataa
    14641 tttttaatcc cggtgccgaa aatgccacct tgcagaaaaa agctaatcat ttaggaatca
    14701 aaacaattaa tgactgcacg ctggtaatgt tgagcgtggg tgcctattga aagatcacgt
    14761 tagttcccat tctccggaat tgttcgataa aagtaatctc attatccacc ttattccaaa
    14821 aaggattttt ttgaaggaac aatagtttca tgttcttcag gtcagcgaga ttttcaggaa
    14881 attcttcgat atcattataa gagatatcca actccctcaa ctttttgagt ttgaggattt
    14941 gcggtggaaa ataattaagt aaattatttt gaagatgaag aatatttagt tcgtttaagt
    15001 ttccaatact ctcggggata taaaacaacc ggttgtgatg aaggtataat tgtttcagag
    15061 aagcgagagc gccaatattt tccggaacaa gaaaaataga attaaaagag agatataaaa
    15121 tctcaagatt tttcaaattt tcaatatcgg aacttatcct ttcgatttta ttataataaa
    15181 gatcagcaat tttaaggttc ttcaacgaat agaatccggg cggtaatgag tcaatagaat
    15241 ttttgtatag agaaatagat tccaaccggg aaagctcact gattctatct ggtaaaactc
    15301 tgatattatt ttcggctaac tgaagttctt tcaggtttac caaatcaaag aggttatcgg
    15361 taaaataatt gatgtcgcat ttattcatgc ggagatactc gatctgtgcc agcttatgta
    15421 acttttgttc atccggtttc aggcggtttt cattcaattt tagtgttacc agattcttgt
    15481 tccttgtaag ccttagagga atccttgtga agaaatttct tgatagatta atttcccgga
    15541 ggcttttaat ttttctgatt gagagcggta atttactgag tccattttcc tgtaaatcta
    15601 taatttctac gttcttcagt cgtggaatct taatactcct gttgcccgaa ttattacccg
    15661 caatctgcag cgttttaagt tccggaaatt ttctaaaata tcggggaatt ctactaagct
    15721 gattgtatga taattttatg gttttcagag ccttacactc cttaaccgac ttaggaattt
    15781 cattcatgtt aaggtaggca aggcttaagt attccagtgt atctttccgg gtattggcgt
    15841 tgaatgattc aaattttacc agctcaagtt gtctgctcat taactctctg gctttacgta
    15901 aggaatcata atccagacga ttaattgcgg gcaggggtga aggttccttt tcaattatag
    15961 tgatagtatc accaggcttg gtaccttccg gaattttaat ttcctgggcc cagattttgg
    16021 atgaaaaaaa gaccagaaaa caggcgaata aagggcttgg ttttagctga aaaaacattc
    16081 cttaaaacta cctgaataag tgccaatttg aaagtgattt ttgaacctta aagtggcttt
    16141 tttttcgtat ttttggagct tcaaaatgtc cacaatgagc gatgaaaaaa cggttgccaa
    16201 aagcagctat tctgcacaaa atatccaggt cttagaaggt cttgaagcag tacgaaagag
    16261 acctgccatg tacataggcg atgttggagt aaaaggtctt caccatttaa tttgggaggt
    16321 tgttgataac tcgattgatg aagctttggg aggatattgt acggatattg aagtacagat
    16381 aatggaagat aattccatta ttgtgattga taatggcagg ggaataccaa ctgacatgca
    16441 tataaaggaa aaaaggtctg ccctggaagt tgtaatgaca gtcttgcacg ccggcggtaa
    16501 gttcgataaa gattcttaca aagtatcagg cggattgcat ggtgttgggg tatcctgtgt
    16561 gaatgcttta agtgaatccc tgaaagcaac cattcatcga aatggaacta tttacgaaca
    16621 ggaatacgca aaaggcttcc cgaaataccc ggttagggaa gcgggtacca ccgggaaaac
    16681 cggcactatt atccatttta agcccgatgt cggagttttt caggcaacgg agttcaatta
    16741 tgagacaatt gcctccaggt tgcgggaatt ggctttcctg aatgccggga tcaaacttaa
    16801 gttgaccgat ttcaggacta aggataatgg aaactataag tcagatgatt tcctttcaga
    16861 aggtgggctc accgaatttg ttaagtatct ggatacatcg aaggaaaaat tgatccccaa
    16921 tcccatattt atcgacacca ttaagggaga tgtcccggtg caggtagccc tgggttacaa
    16981 tacctcgttt actgaaacgg tggtaagcta tgtaaataac attaatacta ttgagggcgg
    17041 tacccatgtt gccggtttca gaagagcatt aacccgaaca cttaagtctt atgctgataa
    17101 atccggctta ttggataaag ctaaagttga aataagtggg gatgatttcc gggagggatt
    17161 gaccgcggta atttcaatta aagttgctga acctcagttt gaaggccaga cgaaaaccaa
    17221 gcttgggaat tcagaggtga tgggagctgt ggacaccgcc gttggtgaaa ctcttcaata
    17281 ctttcttgaa gaaaacccga aagaagcaaa gcagatagta tccaaggtta ttcttgctgc
    17341 ccaggcccgg catgccgcaa ggaaggcaag ggaaatggta cagagaaaaa atgtattagg
    17401 tggaaccggg cttcccggaa aacttgcgga ttgcgccaat aatgatcccg cggtgagtga
    17461 aatatacctt gtggagggag attcagcagg cggttcggca aagcagggga ggaacaggaa
    17521 ttttcaggca atcttaccct tgaggggaaa aatacttaat gtggaaaaag cccaggagca
    17581 taagatctat gagaatgaag aaattaagaa tatgataact gccctgggcg tgagtttcgg
    17641 aaccgatgaa gacgataaag ctgttaacac ggcgaaattg cgctatcata aaataataat
    17701 tatgacagat gctgatgtag acggaagtca tatcagaacc ttaatcctga ctttcttctt
    17761 tcgctatttg agggagttga ttgaaaaagg ctatctgttc atagccctcc cgccgcttta
    17821 tcaaatcaga aaaggcaaag aagagcgata ttgctggact gaagaagagc gggaacgcgt
    17881 agttaaagaa ttggcaaaag atgggaagac agattctgtc gggattcaga gatacaaagg
    17941 tttgggcgag atgaatccgg aacaactttg gtctacaacc atggatcctg aaagaaggag
    18001 tttgaagaaa gtatccattg aatcagcggc ggaagcagag catttatttt caatgttaat
    18061 gggcgatgat gttgcaccca gaagagagtt cattgaaaag aatgccagat atgcgaacat
    18121 tgatatctag tctaaagtac cttttgacgg gtaaggtctt cataaatgga ataaatcaat
    18181 ccatctttta ggcccacttc cggtaccata attgtattta tacccgcata ttccattaca
    18241 gacaagtaaa tttttgcggc aggtattatt acatcggcac ggtccggatt cagctttagg
    18301 atatttattc tatcttcttc cgaatacctg cctatttcat tgatagtggc attaattgta
    18361 ttgacagaaa ttttctttga taactttttg ccggttaggt tatatatttt gttgatatta
    18421 cctccggttc caattccttt tatcccagga tattttcgat gtacatttac tttaacccac
    18481 cttttcatcg tttcccaaat tgaagcggaa taaacctgtt tcaaacttct cacggagcca
    18541 atcataaatg attcgctcaa aatccgctcc cggtttttta agatactcaa ttcagtactt
    18601 ccgcctccca catcaatatg cagatacatc gactcatcga tataaggttt aatagcttta
    18661 ttaatcaata tagcttcctg ggcaccatca atgatttcaa tgggaaaatc gtaagcctgc
    18721 tccgcgagct tggcaatatg ctgtccattt tccgcttctc tcatcgcgga agtagcgcaa
    18781 gctttcacta ctgacacttc atacagatca atgagaagct taaagctgtg cagcagtttg
    18841 agaaacttct ctttgatatc ttcggaaatc ctgccagtag taaaaacatc atgtcccagc
    18901 cgcaagggaa agcggatata ctccaacctc ttaaattctg tatttccatt atgagtcagg
    18961 atgcgatcaa cctgaaagcg aacggcattt gacccaatat caatcgcggc aatcttggtc
    19021 atttcatgaa gacattcatg aaactaatat atcgtttatt aggaaaaata ccgaatcaga
    19081 aataggatgg acattcaagc cccttatgaa gtttgggtat ccggattcgg taatttatca
    19141 taagttcatg ggttcccgaa gaatattgac gcaaagcgct ggtggttttg tcataagcat
    19201 acccaaggtg aaaattttca ttcacctgta attcaaatag c
//
LOCUS       DASWHX010000108        69901 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_468334, whole genome shotgun sequence.
ACCESSION   DASWHX010000108 DASWHX010000000
VERSION     DASWHX010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 69901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 69901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..69901
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_468334"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..791)
                     /locus_tag="VGA21_07195"
     CDS             complement(<1..791)
                     /locus_tag="VGA21_07195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650327.1"
                     /translation="MKRKALNYFFILLVVGSCTSGKKALERGDYYEAVIKSVDRLRSN
                     PTHKKSSETLKQAYPFALNWFEMQVKNLQAGNDRFKWSGIVQNYTSINIMYEEIMRAP
                     GALRIIPSPKNYYSELEIAKNNAAEENYSAGLAELNKGTREDAKSAYRLFLTANQFVS
                     GYKDVAEKIEEAKWMATLKVVIEPIPAVARNLEVSAEFFENKIIEYLHNTEVNEFVRF
                     YTKDEAKAVNLTNPDQIISLEFDEFAVGQIYFKETQIPLEKDSVIV"
     gene            1042..2250
                     /locus_tag="VGA21_07200"
     CDS             1042..2250
                     /locus_tag="VGA21_07200"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ectonucleotide
                     pyrophosphatase/phosphodiesterase"
                     /protein_id="HEX9650328.1"
                     /translation="MFKKLQFLGFVFLLLGHIAISQSRDSYVIMISIDGMRNDYTEIH
                     DAINLKQFASSGAIAKSMLPSFPSKTFPNHYTLVTGMYPEHHGIIANTFYDPASKMTY
                     RGSNPNTVQDGKWYGGTPLWVVAEQQNIKTGCYFWIGSEAEIQDVRPSYYKSYDENIP
                     ETKRVEQVVDWLKLPESDRPRFITLYFELVDDAGHNFGPLAPETKSTVQQIDSIIGQL
                     NDQVAKLNLPVNFVLVSDHGMIQVDQENPIDITGILPKEGVTVVPGDTFILLHSEDEN
                     LIDETYNNLKLFARDFDVYKKGFLPDNFHFNSTPLAGDLTLIARAPKVFGIMGSAIKP
                     GAHGYNSTIPEMGGSFIAWGPDIKKDITIEPFENIHVFPLIARILNLDISSLEIDGRL
                     EVLKEILKED"
     gene            complement(2255..3802)
                     /locus_tag="VGA21_07205"
     CDS             complement(2255..3802)
                     /locus_tag="VGA21_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008631859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GNAT family
                     N-acetyltransferase/carbon-nitrogen hydrolase family
                     protein"
                     /protein_id="HEX9650329.1"
                     /translation="MEITQNTPIDLKVVVRNLKISDYKDLSEAMLLAYKKMGGQPWSK
                     EAMRKLISIFPEGQLCVEVNAKVVGVALSLIVDYDKFGDNHTYYEVTDNYSMETHDPK
                     GDVLYGIDVFVHPEFRGMRLARRLYDARKELCERLNLRSIIAGGRIPNYDKYADQLTP
                     KEYIEKVKMKEIYDPILTFQISNDFHVKKILRNYLPQDNQSQAFATLLEWNNLYYEEK
                     EELLSPKKSTVRIGLVQWQMRTVTGFEELVGMVEFYVDAVSGYQSDFLLFPEFFNAPL
                     MAGYNNLAESEAIRKLAGYTPKMCDTFRELAVSYNTNIIGGSMPLLERGKLYNVAYLF
                     RRDGTWDYQKKLHITPSEVKFWGLTGGNSLKIFDTDSGKIGILICYDVEFPELPRILA
                     DQGMQILFVPFLTDTPNGFNRVKSCSKARAIENECYVAMAGCVGNLPRVRNMDIQYAE
                     SAVYSPSDFYFPNNSVVAEATPNSEMMVIADVNLDLLNELNKEGSVQTLKDRRLDLYQ
                     LKWKGKK"
     gene            complement(3864..6311)
                     /locus_tag="VGA21_07210"
     CDS             complement(3864..6311)
                     /locus_tag="VGA21_07210"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9650330.1"
                     /translation="MINHYLKITFRNLFRTKIHTSINILGLSMGIACAILIILFVKDE
                     LTFDNFHSQASNIYRTFYTAERRDGSMVTNTVTPMVIGATMKQNYPEVEATTIWISFQ
                     AEIEYEEKSFSEEINIASPGFFQIFDFVPVEGSLESALSNSSKIVLTESMARKYFGKE
                     SAIDKTLLIPVGPGKKAFIVSAVIEDVPSNSSLQFTSLVHEENAKDIFPPQMITSWNM
                     IAAESYVLLSDGADAKSLERKFPDMVKTAIGEQLEGRKFDVGLQQLKDIHLNTSMPQG
                     IAQLSDPKYTLILSAIALLILVMACVNFVNLSLGRSFARSREIGIKKVVGAMRFQLVG
                     QFLGEAIWLAIISLVIGLAVSYWMLPIFNELSGKNLEFDFSALNLLMFAGLAIGVGVI
                     AGIYPALVMSGFVPAKILKGSLGIGSGKQGARKGMLVVQLVLSIFLITSTIFMKRQLQ
                     FLQNKNLGYNKEQVIMIPLTVPDARGLQETITKGFDKSERITATLRGLPQVEEVAVST
                     QSFGDAGWMKLSYNDQDQTQHDFFVNTVDANFIPLMQMEITEGRNFIFENESDNRRSI
                     IVNETFVNEFNLENPIGKRIPNEGFADHEIIGVVKDFNFSSLHTIIEPLVLTMNSAIV
                     MSGATGVNVNSSFNPKIALRIRAGEIGDGLIAVQNMWKEIYPDDPFNYSFIDEAIKEQ
                     YQQEGNLGQIVTSATILSIVIGSLGLFGLSALTMTARMKEISIRKVLGATLGNLIFSL
                     GRSYLFLTLASTLIAAPITIYFISNWLKTFEYKIGLEPYIFIAGGSIAILICLFTIAY
                     QAIKIALSNPIGNLRTE"
     gene            complement(6396..7256)
                     /locus_tag="VGA21_07215"
     CDS             complement(6396..7256)
                     /locus_tag="VGA21_07215"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEX9650331.1"
                     /translation="MKYRSKINFSAGLINLFPIIIIMTMPALSFSQSIKVLTYNIRYD
                     NPEDGENAWVHRRSEMVKFLLEQNPDIMGFQEVLNRQLEFLKTGLDQYHSLGVGREDG
                     ISKGEFSPLFFKKEKFDLLDQETIWLSETPEKPGIGWDAALERIATVVVLRVKETERQ
                     VVVINTHYDHRGELARLESSKLLLDIIDKRYPGKPLILMGDLNAEPDSSPVKIFSEEL
                     MDTFLCKEKKGPNLTFSGFTNMNYDTGPRIDYILIKNFDCLFFQILTPGIGDRNLSDH
                     LPVISVVEIH"
     gene            7292..7885
                     /locus_tag="VGA21_07220"
     CDS             7292..7885
                     /locus_tag="VGA21_07220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650332.1"
                     /translation="MKSILIIIGLIIVPAISYSQEVENTNKNLREQYQDLKETTETYN
                     QYKVIPENRLDGWFKTIQDSISDKSNRINSLLTSTTALKDTISDLRNEKSQMQTELDE
                     GDFEKSHIGVLGVNFTKAAYIGITFILIIGLSILIILFVIKVIDTGKTTRDTIKLHDT
                     LTAEFEEYKRRALEKQTKLARDLQTERNRIDEIRRKR"
     gene            complement(7889..8662)
                     /locus_tag="VGA21_07225"
     CDS             complement(7889..8662)
                     /locus_tag="VGA21_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009580681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650333.1"
                     /translation="MKLLRSIYLIINYKTFIVTILSLVSTWLCEYYQIVADFPLNIVG
                     LAIVFPVVFSIDSAYKRRERALGYLADFKGHCLAIYHASRDWVDAGSTEFHIKIQDNL
                     IQIYGSVRDLFMSKRKDSDKFEKKIFDEISGLSTLLQDFRKHGLQPGEVSRVSQYISK
                     ISIALENMKNILHYRTPITLRAYSKVFIYTFPIIYGPYFVFASQEYSVGLEYIMPVLF
                     SFILVSLDNIQVHLEDPYDQVGEDDIKFDVEEFERNISV"
     gene            complement(8741..9343)
                     /locus_tag="VGA21_07230"
     CDS             complement(8741..9343)
                     /locus_tag="VGA21_07230"
                     /inference="COORDINATES: protein motif:HMM:NF015871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HEX9650334.1"
                     /translation="MKNISLIINVLLLIAVGLLYLFQFQKEEIKEQAEPINESVIGDM
                     KIAFVNTDTLLKYYSLSDVLMKQLEVKRDKFQAEYLNREKGLQAEIDDYQQNAGNLTM
                     GQARNIEENLMKKQQNLQQYSQTLAQDMAREQNRVNTQLYDSVANFLGKYGTEQDIKI
                     VLTYTKGNPNVLYAANALDITQILIDGLNTRQESQKNPGK"
     gene            complement(9399..10121)
                     /locus_tag="VGA21_07235"
     CDS             complement(9399..10121)
                     /locus_tag="VGA21_07235"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017731695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEX9650335.1"
                     /translation="MSNDAIRFRRGIWISITFILLLWTVQAFEVAFSMDLREFGILPR
                     TLKGTIGIITGPLIHGDFFHLVSNTFPLIILGVGLFYFYHKIAFEVILIIYIMTGFWV
                     WVVARDAYHIGSSGLVYGLLAFLFFSGLLRRDAKTLAVSLVLLFIYGGNMIYGIFPVN
                     EAISWESHLMGALSGMFCAYFFKNSRSSVDTILEDETSEHEMVPDEPAPSGQSYTIVW
                     KKEKKIGSSPTYNYDVDKEDLI"
     gene            10415..11344
                     /locus_tag="VGA21_07240"
     CDS             10415..11344
                     /locus_tag="VGA21_07240"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEX9650336.1"
                     /translation="MHLHRHEGSVEKKGFRLWLAIILNVGITFAQVVGGIFAGSLALL
                     SDALHNLSDVLALSISLFARKLVHKKSSSSKTFGFKRAEILAAFINAASLVVVSAYLI
                     MEAIKRLIQSPDEIVDGNLVMIMAGVGIVGNALSVAILHKESGENMNMRSAYLHLISD
                     MLFSVAVLAGGFVIVKFGIYWVDPALSIVIAVYLIFISWKLFYKSLTVLMQFAPGEID
                     LTQVAAVISKYPEIDNIHHIHAWELNEDEIHFEAHIQIKENITIDKVCELLQRIEEEL
                     QSEFGLTHITLQPEFNRDCGEELFVEEHHHHSS"
     gene            complement(11332..11844)
                     /locus_tag="VGA21_07245"
     CDS             complement(11332..11844)
                     /locus_tag="VGA21_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020532944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HEX9650337.1"
                     /translation="MALIKNVRGIKPTIGNNCYLAENATITGEVTIGDNCSVWFNAVL
                     RGDVNSIMIGNNTNVQDGAVIHGTYQKAKTVIGSNVSIGHNAIVHGCTIEDNVLIGMG
                     AIVMDHAVVQSGSIVAAGAVVLMNAIVESGYIYAGVPAKKLKTVGDLGNVFERTSKNY
                     IMYSGWYQEE"
     gene            11990..13420
                     /gene="miaB"
                     /locus_tag="VGA21_07250"
     CDS             11990..13420
                     /gene="miaB"
                     /locus_tag="VGA21_07250"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017731682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HEX9650338.1"
                     /translation="MEPVIKDINILDDNREESCEVVKVSEENNAGNTRKLYIESYGCQ
                     MNFSDSEIVSSIMAKEGFDTTSSLENADVIFINTCSIRDKAEQTIRSRLAFFNSLKKQ
                     KPELQIGVLGCMAERLKAKLLEEERIVDLVAGPDAYRDLPNLVRQVDDGQKAVNTFLS
                     REETYADISPVRLNSNGVTAFISIMRGCDNMCSFCVVPFTRGRERSRDPETIVNEALN
                     LYNNGYREVTLLGQNVDSYRWGDKENPQVNFAQLLGKIAAVSPELRVRFSTSHPKDIT
                     DEVLETIARYENICNYIHLPAQSGSTRVLELMNRTYTRDWYLDRIGAIRRIVGNECGI
                     SSDFIAGFCTETEADHEYTLSLMDEVRYDFSYMFVYSERPGTLAERKFKDDVKPDIKQ
                     RRLREIIDKQQEHSLKRNQRELDKVHKVLIEGYSKRSEEYMQGRNSANKVVVFPRENY
                     KKGEYVNVRVTDCTAATLMGKAVQDD"
     gene            13413..14672
                     /locus_tag="VGA21_07255"
     CDS             13413..14672
                     /locus_tag="VGA21_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008201635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX9650339.1"
                     /translation="MINDKEIQSIKQRFGIIGNSPSLNHAIAVAMQVAPTDMTVLITG
                     ESGSGKESFSKIIHALSPRKHGQFIAINCGAIPEGTIDSELFGHEKGSFTGAYEARKG
                     YFEVTSGGTIFLDEIAEMPLNTQARLLRVLEYGEFIKVGSSKVLKTDVRVVVATNVNL
                     SDKIKKGKFREDLFYRLNTVPIAVPSLKERGDDVELIFRKFAADFSEKYKVKPISLTE
                     EAKQYLLGFPFPGNIRQLKNLVEQISVLETDREISREKLMKYLPSSSSSLPMLFDKST
                     INNEGMTERDLLYKVLFDLKRDVNDLKKLVYSNISGDVSSAKLIKDHQELFDSIEEKP
                     ARQKEQSPVLLNLDSNEAKSYELEKVEDIMHETEEDEELSLDKKEKEMIIKALRKNNN
                     KRKYAAHDLGISERTLYRKIKQYEIEE"
     gene            14656..15180
                     /locus_tag="VGA21_07260"
     CDS             14656..15180
                     /locus_tag="VGA21_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009195807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptE family protein"
                     /protein_id="HEX9650340.1"
                     /translation="MKLRSRFFLIFPFILTGCGIYSFTGTTLPSSIRTFSVQEFFNDT
                     DGGPPNLGLTLTNEMRDYFQSNSSLTLVQEEGDLQFEGGIIEYRLTPVAPTNTGTGIN
                     NITSSSVTRLTIVVRAAFTNTVDETQSFNRSFNFYADFNSVTETLNDAENRLIETIFE
                     QIILDIFNASVAVW"
     gene            15234..16340
                     /locus_tag="VGA21_07265"
     CDS             15234..16340
                     /locus_tag="VGA21_07265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650341.1"
                     /translation="MNKDKFITLLRNYRNLSGEDLKELSLLSDEFPFSQALHNLVAKG
                     AMDMKAPEAKTKLGTAAIYSPDRAALKRLIEGSVQFVAAVHEESLDRSIETPAATPKT
                     ETPPPPPEPEKIDKPVTQDKVPHTEMPAAEVKTPEPVSKPASVQDLVKDPTHQAKVEA
                     LRNEIFSNLKELQKNIVDSNKVFDFYDKKHENADAAKKKAVQDKGRELKELEESAKAK
                     LSGREKAEDKEITMGTEAAPVKEAAPETPTKPKSVRKKTISKPKKTKSKSPANRTNKS
                     AIKKKPATGKKKTSKKAAGKTTKVSGRKTAKKKTISRKAPVKKAASKKKAATRKKLKK
                     AVGKSTKTTGSSRKKTTARSSKKTVKKKVNRTRK"
     gene            16466..16663
                     /locus_tag="VGA21_07270"
     CDS             16466..16663
                     /locus_tag="VGA21_07270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9650342.1"
                     /translation="MSEIREDLTANITKISDSLLTENLAEIFLQQGKSDKAIEIYKKL
                     IWKFPQKKAYFASQIDKLSRR"
     gene            16663..17043
                     /gene="secG"
                     /locus_tag="VGA21_07275"
     CDS             16663..17043
                     /gene="secG"
                     /locus_tag="VGA21_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009582285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEX9650343.1"
                     /translation="MFTLVISLIVIVCVLLVLIILAQNPKGGGLSSQFGGSGASNLIG
                     VKKTGDLLERLTWGFAITLFVLSMATNFIIPSNTGTGLNSPNIDRAEEQQQTPELDLE
                     GIQEESQPSDNSSLEDLLEGDSTK"
     gene            complement(17049..17777)
                     /locus_tag="VGA21_07280"
     CDS             complement(17049..17777)
                     /locus_tag="VGA21_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009582286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650344.1"
                     /translation="MKELSKNWLTEGLIDFEYKKYYLLAYLKEVKSGFSRMEMFPGLS
                     DLVFHYRNLMQLRDNKELLYGSFPQSISKADFEKLELSYKKIIKDDEVMKEIQDIIEF
                     ALPTMKQALEEGRDIYEFIESNFEISTIGLCPLYVDEGYLFLYIDSSSEAHVHKYAMS
                     VFENSDEKFRGITTQWIETFTRSLYSSFERKKLELIRKFKLLPNPATYLIVSKTVFPY
                     DATLMPIAKRLLIRHISSTGKLQS"
     gene            18019..18297
                     /locus_tag="VGA21_07285"
     CDS             18019..18297
                     /locus_tag="VGA21_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009582287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEX9650345.1"
                     /translation="MSKVKIKPLADRVLIAASPAEEKTASGLIIPDTAKEKPQKGKVI
                     AVGMGKKDEPITVKVGDTVLYGKYSGTEINIDGKDYLIMRESDIFAII"
     gene            18346..19974
                     /gene="groL"
                     /locus_tag="VGA21_07290"
     CDS             18346..19974
                     /gene="groL"
                     /locus_tag="VGA21_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010610187.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEX9650346.1"
                     /translation="MAKEIHFDSAARENLKKGVDTLANAVKVTLGPKGRNVILDKKFG
                     APTITKDGVTVAKDIELKNPLENMGAQLVKEVASKTADDAGDGTTTATVLTQSIFTHG
                     IKNVAAGANPMDLKHGIDLAVAAVVDSLQKQSKPIKNSSEITQVASVSANNDEEIGQM
                     IADAMDKVGKDGVITVEEAKGTETEVKTVEGMQFDRGYLSPYFVTNADKMEAELESPF
                     ILIYDKKISSMKELLPVLEQVSQTGKPLLIIAEDVDGEALATLVVNKIRGALRVAAVK
                     APGFGDRRKAMLEDIAVLTGGTVISEERGYKLENAGLDYLGSAEKINIDKDNTTIVNG
                     AGKKNDIKARISQIKAQVETTTSDYDKEKLQERLAKLSGGVAILYIGAATEIEMKEKK
                     DRVDDALHATRAAVQEGIVAGGGVAFVRAIKALSKLEGRNEDQNTGINIIRLALESPL
                     RIIVENAGMEGSVVIQKVKEGKDDFGYNARDNKYDYMFKAGIIDPTKVTRLALQNAAS
                     IAALLLTTEAVVSEVPEEEKAPAMPHGGGMGGMM"
     gene            complement(20148..20525)
                     /locus_tag="VGA21_07295"
     CDS             complement(20148..20525)
                     /locus_tag="VGA21_07295"
                     /inference="COORDINATES: protein motif:HMM:NF020903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SiaC family regulatory phosphoprotein"
                     /protein_id="HEX9650347.1"
                     /translation="MDDKLDIPATEINPGVYLDSLSGKMKIEGKSISENPENFFSDVY
                     DWLETHLPSFPDEIEFRIHLSVVRVQSLLEIARLLRLLEKNIMHKKKIRITWIYKTDD
                     EEMKESGLLVDKMIRNDLEFENH"
     gene            complement(20544..22034)
                     /locus_tag="VGA21_07300"
     CDS             complement(20544..22034)
                     /locus_tag="VGA21_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013452358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HEX9650348.1"
                     /translation="MTEGKTFFGHPRGLATLFFTEMWERFSYYGMRAILILFMTTPAA
                     QQGLGLNNETSGPIYGLYTASVYLLTLPGGWLADNIFGQRKAIWYGGIMIMMGHLILA
                     IPGSVPIFATGLAFVACGTGLLKPNISTIVGDLYPEGGARRDAGFSLFYMGINTGSIL
                     GQFVVPLVGEKINWHWGFGLAAVGMFFGLLQFRLSQDTLLNLGIKPKAKERQEEGGEI
                     TGNKGMAIGLVIALIAFLVVLQYVNVIDLTSAGGIAAGAGVLIVSITLFYFLYILIAG
                     GLNGEEKRRVGIIFFLFIGAALFWAGFEQAGSTLNLFSRDYTNRFLFGWELPAGWLQN
                     VNPLMIVIFAPIIGALWVKLAAKNLNPATPLKFGLGLILMGFGFLVMYFAAHRVVEGM
                     QAGMAWLVLTYFLHSAGELALSPVGLSATTKLAPKAFVGQMMGIWFVATSLGNLIAGM
                     FAGEFDPNNVSGMPSLFMTVVLFGCGSGILFVIFSPLMKKWMGNVQ"
     gene            22273..23886
                     /gene="pckA"
                     /locus_tag="VGA21_07305"
     CDS             22273..23886
                     /gene="pckA"
                     /locus_tag="VGA21_07305"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017731671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HEX9650349.1"
                     /translation="MKEFGFKSEKCGLNEIGIDNPDSVYWNLSPAELVEEGLRNNEGV
                     LTNTGAFMCDTGKFTGRAPKDRYIVEDETTSETIWWGEINIPITQKKFDNLFNKIVKH
                     LDGKKLYVKDAYAGSDKKYRLNLRVINTFAWHNLFCHNMFLRPTQPELKSFIPTFTVI
                     NAPDFLADPKMDGIKNPNFVIINMTKKVIIAGGTGYAGEMKKGIFSVLNYLLPQNHQV
                     LSMHCSANIGEEGDTAIFFGLSGTGKTTLSADPQRGLIGDDEHGWTENGVFNFEGGCY
                     AKAIDLTREKEPQIFDAIRFGAILENTRFKEGSRDVDYTNVSVTENTRTAYPIDHIDN
                     AVIPSIGGIPKNIFFLTCDAYGVLPPISKLNKGQAMYHFISGYTAKVAGTEAGITDPQ
                     TVFSACFGAPFLPLHPTKYAEMLGKKMTDNDVNIWLVNTGWSGGPYGIGERMKLKYTR
                     TMICSALEGSLQSSEFKKHDIFGIEFPTSCPNVPPEILDPKNTWKDKEGYDNKASYLA
                     KAFIKNFSKYENFANEEVLQGAPKPNVAV"
     gene            24179..25645
                     /locus_tag="VGA21_07310"
     CDS             24179..25645
                     /locus_tag="VGA21_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008200513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HEX9650350.1"
                     /translation="MKKTFRSRLPYLILLLILSLSQSVQSEEFNLSETTNMEVTQNSQ
                     PESEIVPVGDKTDQSLGVSLLRGLLGVSSIILIAFLFSSNRKQINWKVVLSGLGIQLI
                     LAFGILNINFIQKLFEFVSSIFIAILDFTKAGSTFLFGDLLNTDSIGFVFALQILPTI
                     IFFSALTSVLFYLGVIQKIVYGLAWLMTKALRLSGAESLSVAGNIFLGQTESPLMIKA
                     YLEKMNRSEILLVMIGGMATVAGGVLAAYVGFLGGDDPAQQLLFAKHLLAASVMAAPG
                     AVIISKMLVPQTQAIDSNIQISYDSAGSNMLDAMANGTTEGLKLALNIGAMLLVFFAF
                     IALFNHVLGWVGTTTGLDTAIAGFSSGRYDNLSLEFILGYAFAPLMWLLGVASHDITL
                     VGQLLGQKIIASEFVGYESLATLKNSGVLTDTKSIIMSTYMLCGFANFASIGIQIGGI
                     GGLAPGQRVLLSEFGFKALLGGTLASLLSATIVGMILG"
     gene            complement(25642..26232)
                     /locus_tag="VGA21_07315"
     CDS             complement(25642..26232)
                     /locus_tag="VGA21_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015810745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease domain-containing protein"
                     /protein_id="HEX9650351.1"
                     /translation="MDKIKLEILGLSSSQSQTGSFALVLGEEKGNRRLPIIIGMFEAQ
                     AIAIEIEKIIPNRPMTHDLFKSFAASYKYQVEEILISDLKEGVFFAKIVCSNGKGTIE
                     IDARPSDAIAIGIRFDAPIFTYETILAEAGIVLTEEAEGVASLKTEIVKPKKHETIAS
                     LKDLSIDKLNEILKEAIDDEDYERAAKIRDEINKRN"
     gene            complement(26240..27196)
                     /locus_tag="VGA21_07320"
     CDS             complement(26240..27196)
                     /locus_tag="VGA21_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009582298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HEX9650352.1"
                     /translation="MSVLVFIESSDKKVKKTSQEAVSFAAAIDKNAVAIYLGSDITTE
                     ELEALGKFGAGKVLHVNNDKLNQGVIPAYATVISEAMRKTDSKILVLANSSLGAPVAA
                     RTAVKLNASLASNVTELPDTSTGFRVKRSIYTGKAFAVTELNNEYKILAIRKNAVQLT
                     ESGSKAPVETFSVDLNPADFALKITGTDQASGDVLLPEAEIVVSGGRGMKGPENWGML
                     EELAKELGAATACSKPVSDMEWRPHHEHVGQTGLKVGPNLYIAIGISGAIQHLAGVNS
                     SKVIVVINKDPEAPFFKAADYGIAGDAFEVVPKLTEAIRASK"
     gene            complement(27205..27942)
                     /locus_tag="VGA21_07325"
     CDS             complement(27205..27942)
                     /locus_tag="VGA21_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014772965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HEX9650353.1"
                     /translation="MKILVCITHVPDTTSRISFTENNTKFNTNGVQFIIGPYDDYALA
                     RAVEIKEATGASLTVLNVGGPESDPTIRKALAIGADDAIRVNANPADSFFVSNEIANV
                     AKEHNYDMILMGRESIDFNGGMVHGMVGELLGIPSISPVMSLELDGTKAKMAREIEGG
                     KEYVEVELPFVAGCQEPIAEWKIPNMRGIMSARTKPLNVVEPKGNVQSTKLVSYELPP
                     AKGQVKMIDPENVSELVKLLKNEAKVL"
     gene            28352..28873
                     /locus_tag="VGA21_07330"
     CDS             28352..28873
                     /locus_tag="VGA21_07330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650354.1"
                     /translation="MEVINKKSIISIFVLIILVLILIAVFYNNNPHQEKQINLQGIAS
                     RYLKAYPLPAGWNNDPNLSDKYAAFRLYSQRNYLEAMVNLKYTLVNNPGDKEVNFYLG
                     ICMLLTNKPRDSIREFNIIIESENDPLINDAKWYKALALIKLDRIPQAKRLLGELSSE
                     DSIKLLDEISVGN"
     gene            complement(28870..30390)
                     /gene="dnaB"
                     /locus_tag="VGA21_07335"
     CDS             complement(28870..30390)
                     /gene="dnaB"
                     /locus_tag="VGA21_07335"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009053764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEX9650355.1"
                     /translation="MERKTSSIRLGYKNPVFSASDINEGIGKIPPQALEIEEAVLGAL
                     MLEKDALSTVIDILKPESFYKDSHQEIYKSIQILFNNSEPVDILTVTNQLKSDGKLEF
                     AGGAYYITQLTSKVNSAANIETHARIITQQAIKRELIRIASEVHRDAYEDTIDVFDLL
                     DRTEQSLFDVSETNIRRNYADMRSIMKEAMDELEARKHHKDGLTGVPSGFTNLDRITN
                     GWQKSDLIILAARPGMGKTALVVSAMRNAAVDFEKPVAIFSLEMSSVQLVNRLISAEA
                     ELESEKIKKGNLVEFEWEQLVHKTAKLTSAPIFIDDTPALTIFELRAKSRRLKAQHDI
                     QLVIVDYLQLMSGDMSKNSGGNREQEIASISRALKNLAKELEIPVVAISQLSRAVETR
                     GGDKRPQLSDLRESGSIEQDADLVIFLYRPEYYGITEDENGIPTASMAELIIAKHRNG
                     SLDSIPLKFIAKYTKFTEPDTHSSFSGSGSSGSTVTFGSRMNAGRDEDDLPGPAPF"
     gene            30704..32071
                     /locus_tag="VGA21_07340"
     CDS             30704..32071
                     /locus_tag="VGA21_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017731654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEX9650356.1"
                     /translation="MNKELNRIHFIAMGGSVMHQLAIALAREGRNITGSDDEFFEPSK
                     SLLEKSGLLPVKAGWFAEKITDKLDAVILGMHARRDNPELQKAFELTIPIYSFPEFIY
                     LHSKDKQRFVISGSHGKTTITAMIIHVLNYYKKEFDYVIGAKVDGLDSWVKLSDAPVI
                     ILEGDEYASSPLDKTPKFMKYKHHIGVINGIAWDHMNIFHTEEAYLRAFEDFADATPK
                     AGSLFYCEEDSLTSMIGIKQRVDVNSIEYKSFPFETKNGNIFIKDETELFPVKVFGKH
                     NMQNMGAAQAVLRRLGISSKEFLQAMGSFTGASSRLEVLAQTKSATVYKDYAHSPSKV
                     LATMDAVREQHPGIKFIACFELHTYSSLNSEFIKNYKDSLNKADQAIVYYNQETSLHK
                     KLNPLTSEEIKNSFNNEKLAIVNNSSDLKAQLDSLSGTFVLLLMSSGNFGELNLKEIA
                     QKITS"
     gene            32071..32874
                     /locus_tag="VGA21_07345"
     CDS             32071..32874
                     /locus_tag="VGA21_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013452282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEX9650357.1"
                     /translation="MIINLRNPIAFFDLETTGVNISQDRIISISLIKIFPGNEQEKKE
                     YLINPGIPIPAESSLIHGIYDEDIKGKPSFKEIARNLAKFLEGTDLAGFSIINFDVPM
                     LVEEFLRAEVEFDIRKKKIVDAQKIFHLMEKRTLTAAYKFYCNKELANAHNASADTLA
                     SLEVLEAQVQKYSGNEVTDLLGNPLGQLENDMEVLHKLTISNKVDLAGRLAFDDNGIE
                     VFNFGKHKGKKVTDIFEQEPQYYDWIMKGDFPLDTKRRITEIKLRGFKK"
     gene            complement(32877..33674)
                     /locus_tag="VGA21_07350"
     CDS             complement(32877..33674)
                     /locus_tag="VGA21_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008630479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEX9650358.1"
                     /translation="MISRLFSGALIILGVFSCAKVPVTGRSQLSLISSQELMIMSFES
                     YKEVIAESKLSQNQQQVDMIKRVGTRIKDAVEVYLTEQGSANQLQGFLWEFNLIEDDT
                     TINAWAMPGGKVAFYTGILPVCQDELGVAVVMGHEVAHAIANHGRERMSQGLLANLGL
                     STLSMAMGQNPSLTNDIFLQSIGIGTQMGMLKFSRNHESEADHIGLIFMAIAGYDPQA
                     APAFWQRMTSGTGGQPPEFLSTHPSHERRISDLNAWMPEAMGYYKKN"
     gene            33798..34409
                     /locus_tag="VGA21_07355"
     CDS             33798..34409
                     /locus_tag="VGA21_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010610817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX9650359.1"
                     /translation="MKIFGIGRNYAEHIQELKNETPDDPVIFFKPDTALLRNNDPFYY
                     PDFSKDIHYEVEIVLRICKNGKNIESKFARTYFDQIGIGIDFTARDLQEKAKEKGLPW
                     AIAKGFNHSAPISEFYPVGDFKDLDNLEFGLEIDGVLKQKGNTSMMLFGYDKIITYIS
                     KFFMLKIGDLIFTGTPKGVGPVKIGNRLRAFLEDKTLLDFEVK"
     gene            34396..36294
                     /locus_tag="VGA21_07360"
     CDS             34396..36294
                     /locus_tag="VGA21_07360"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX9650360.1"
                     /translation="MKLSKCLSFLVLIYFPILAQNGNGESDYRRYILPVFPNQTNTLA
                     GTMGELRNTHFHTGIDIRTQGKTGLPIVAAADGYINRISVQGSGYGNALYIQHADGNI
                     TVYAHLNNFNDEIAEFVRQEQYKRQKFEINLFPAQNQFPVRQGDLIALSGNSGSSVGP
                     HLHFDLRDENQNLLNPLEYGFHEVIDDIPPVARKIAVSTLDINSRIAGEFGRMEFDLI
                     RQGDKYTVADTLLASGLIGLEIYAYDRQNYTFFRTGIPTFKVFINKQQVFNQNIIAFS
                     FSDQKHINVHTNYQKKVDTGRSFAKLYIDDGNELPFFQAFNKGKIKVTEGVVLPVTFE
                     LKDTYNNSTIVEFFIKGQKSAGSYKSTSKISSGIRSEIKENTLIIRCNKDSLFNNQLK
                     YFSKGMESLAYPAYITGNGSAVFLFDLRKVLVDSVSISNSKKYYNFKAMVPSGIPYSF
                     YSNSIDINFSRNSLFDTVFLATRYFVNDNNQEIFHINENTEPLKNSIKVTLKTRNSYP
                     SKTEVYTIDSKGNLGYMGGSWDGKSIIFSTTTFGDYTLASDSIPPVIRPVIVNSNNLT
                     FIIEDKMSGIKEFRAMVNGKWLLMNYDPKKNLIWSEKLKPETRLAGDVILKVTDKAGN
                     ESIYQTKL"
     gene            36300..36749
                     /gene="bcp"
                     /locus_tag="VGA21_07365"
     CDS             36300..36749
                     /gene="bcp"
                     /locus_tag="VGA21_07365"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014773503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HEX9650361.1"
                     /translation="MTLNVGDKAPDFKIKNQDGKTVSLKDYKGKKIVLYFYPKDNTPG
                     CTSESCNLRDNYSALRKKGYEVIGISSDSEKSHRNFIKKFQLPFTLLADIEKEVHHHY
                     GTWVEKSMYGRKYMGTARVTFIINEKGIIEKIIDKVKTGDHTNQILT"
     gene            36749..37600
                     /locus_tag="VGA21_07370"
     CDS             36749..37600
                     /locus_tag="VGA21_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013453131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEX9650362.1"
                     /translation="MIDQKEIQKLKDLTFQVRRDIVRMVHAVQSGHPGGSLGCTEFFV
                     AMYFHHLRHNNDFSMEGKNEDMFFLSNGHISPVWYSVLARSGYFPLSELKTFRKIDSR
                     LQGHPATHEGLPGVRIASGSLGQGLSAAIGAALAKKLNGDPHFVFVLMGDGEQQEGQV
                     WEASMFAAHYKIDNLIASIDVNGQQIDGPVNEVISLKNLKAKWEAFGWNVIESNGNDL
                     ENIIDKIEEAKSLTGKGQPVLNLMTTVMGKGVDFMEGSHKWHGIAPNDEQLESALAQL
                     EETIGDY"
     gene            37627..38583
                     /locus_tag="VGA21_07375"
     CDS             37627..38583
                     /locus_tag="VGA21_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017731642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEX9650363.1"
                     /translation="MKTKFTFTEKKDTRSGFGAGLLEVGRGNDQIVALCADLTGSLKM
                     DAFQKEFPERFFQTGVAEANMMGIAAGLATSGKIPFTGTFANFSTGRVYDTIRQSIAY
                     SGKNVKICASHAGLTLGEDGATHQILEDIGMMKMLPGMTVINPCDYNQTKAATLAISE
                     HYGPVYLRFGRPGWPIFIPAETKFEIGKALPLIEGSDVSIFATGHMVWQAIEAEAILA
                     EKGVSAEVINIHTIKPLDKEAILDSVAKTGCVVTAEEHQMNGGLGESIARLLAQKRPT
                     PLELVAVQDTFGESGKPLDLLKKYGLSPENIVGAAIKVINRK"
     gene            38649..39836
                     /locus_tag="VGA21_07380"
     CDS             38649..39836
                     /locus_tag="VGA21_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008200299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEX9650364.1"
                     /translation="MTTSRKTFFELQAQTSPHPFGLEIERAEGLYIFDRQGRKYMDMV
                     AGLAVTNIGHRHPKIVEAIKKQADTHLHITPYGEFIQLPQIILAEKLASILPANLNCS
                     YFVNSGTEANEGAVKLAKRATGRRKVIAFHKSYHGSTHGSLSFSGNEKKKSAFRPLLP
                     EVYFLKFNSEEDLELIDNKTACVIMETVQGDAGVRIPSHSFMKKLRDKCSKTGALLIL
                     DEIQTGFGRTGKMFAFEHYSIVPDILTLAKALGGGMPIGAFISSKEIMAKLSYDPMLG
                     HITTFGGHPVSCAAAAANIGVLLDEKLIDPVEQKGALLQELLDHPKINEIRRIGLMLA
                     LEFDSRETVEKIVKGCMENGVICFWFLSCPESFRLSPPLTISEEEMEIAALVIKQEIE
                     SHT"
     gene            complement(39970..41130)
                     /locus_tag="VGA21_07385"
     CDS             complement(39970..41130)
                     /locus_tag="VGA21_07385"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX9650365.1"
                     /translation="MKTLVLSVLITTGATFILISCDKKTDLVSLPTRSVTITGEVQNI
                     QPGLILLEEFVLQSNSYLVYDTIPLNQNNTFSREIYLSNPGFFRLNFFNKQFITVILD
                     EDDIHVAADGGSQNGISQITGSSDVEKLREIATLVEEYRQLESELNQEYAQAVAVNDS
                     EKAQKLTGEYMLLQGEKTEMIKQEIERMGISLATVQALSLINKDEEFEFFDNVVSKLI
                     QKYPNVPFVQNLANEVERLRAVAIGQMAPEISLPTPEGGIFKLSSLRGDYVLIDFWAE
                     WCKPCRMENPNVLRAYNRFKDQGFKILGVSLDRSRDKWLKGIAEDGLPWNHVSDLNYF
                     NSEAARAYNVSAIPFSVLLDPEGKIIAKNLRGKDLHTKLEEIYGRDTSAPGR"
     gene            41424..42092
                     /gene="fsa"
                     /locus_tag="VGA21_07390"
     CDS             41424..42092
                     /gene="fsa"
                     /locus_tag="VGA21_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019597736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEX9650366.1"
                     /translation="MKFFIDTANLSEIQEAFDLGVLDGVTTNPSLMAKEGVRGKDNIK
                     AHYKAICNIVDNNVSAEVIATDFDGIIREAHELAKIDNKIVVKVPMIKEGVKAIKYLS
                     TEGIRTNCTLIFSAGQALLAAKAGASYVSPFIGRLDDVSSDGVALIGQIVLIYSNYGY
                     KTEVLAASIRHTMHLIQCAEIGADVATCPLKVITGLLQHPLTDKGLAQFLADHKKANE
                     KVSV"
     gene            42095..42805
                     /locus_tag="VGA21_07395"
     CDS             42095..42805
                     /locus_tag="VGA21_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009183776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX9650367.1"
                     /translation="MSFSSDPVVRVQNATIFQDEKAVLEDINFEVAKGEFVFLVGRTG
                     SGKSSLLKTLYADLPLRLGDIEVTGYDIRKIKSKDIPYLRRKIGIVFQDFHLFPDRSV
                     AENLYFVMKATGWRDNSKMKSRATDVLMQVGLGAVSGKMPHQLSGGEQQRVVIARALI
                     NEPAIFIADEPTGNLDPAVSEGIFKVFQEINNSGTAVMMATHDHYFIKKYPNRILKCS
                     EGKVQDSRNKEINLEEML"
     gene            complement(42802..43332)
                     /locus_tag="VGA21_07400"
     CDS             complement(42802..43332)
                     /locus_tag="VGA21_07400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650368.1"
                     /translation="MKDRFEKFVSENRESFDLETPGDRVWKRVQREISGKRASTSGFW
                     MWKAAAVFFFFLSAFLLLNDFLINSERAGKLTGNTDQFIETENYYTSLISLKEQELDN
                     YADNVDFDYMAQLGNLNAIYQVLKTELEKDPSQPVIDALILNLISRVDILNRELDRLE
                     KMQEDEALEERPATKS"
     gene            complement(43322..43888)
                     /locus_tag="VGA21_07405"
     CDS             complement(43322..43888)
                     /locus_tag="VGA21_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009582217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX9650369.1"
                     /translation="MDSPMGNDYVNIHQDLVDACLAGDESAYSELYKLYSKAMFNVAL
                     RIARDSDDAEDILQDAFVSAFTNLHSFSGKATFGAWLKRIVINKAINHLKKQKANLVE
                     LSVDYELEDKVENEPDMEFKVKTVQKAIQELPDGYRVVLSLYCLEGYDHKEIAQIMGI
                     SESTSKTQYLRAKQKIVDLIGKERTYER"
     gene            complement(43944..44618)
                     /locus_tag="VGA21_07410"
     CDS             complement(43944..44618)
                     /locus_tag="VGA21_07410"
                     /inference="COORDINATES: protein motif:HMM:NF024579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9650370.1"
                     /translation="MNRIVVILVMMVISLSGFSQTGKQDYERFKKVYDRAMKYNDFPV
                     AKNALYNMLAADPENDSVLYTLAYYYFDSQSYASCLMVCMDIIAKNPNELGIIEAMAV
                     SYENLGLLQKSLEAYEKLYLLTDNINSLYKTTFIQFELKRYDECETNIDILLSKKEVD
                     ELKLVYTFEENKQEEFPMRVSVLNLKGALLIQQGDTVEARKTFEEVLALAPNFKLAKE
                     NLASLK"
     gene            complement(44620..46065)
                     /locus_tag="VGA21_07415"
     CDS             complement(44620..46065)
                     /locus_tag="VGA21_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013455518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEX9650371.1"
                     /translation="MQFKSYISGSPMLSLVAEVASDEGIETYVVGGYVRDLLLKKNGK
                     NGKDLDFVCIGSGIDLAKKVKAKSGKGSLATFKNFGTAMLKIEDQELQFVGARKESYR
                     ASSRKPIVEDGTLEDDQNRRDFTINALAISLNENDLGTLLDPFGGIKDLKKKIIRTPL
                     DPKITFSDDPLRMIRAIRFSAQLKFDIEPDTFEAIINNAERINIVSQERITDELNKII
                     LSENPSYGLNLLYESGLLKIIFPEIVELAGVEIIEGLAHKDNFYHTLKVLDNVAKYSD
                     DLWLRWAAILHDIAKPATKRFDKKAGWTFHGHEDKGSRMVPGIFRRLKLPLNENMKFV
                     QKLVKLHLRPISLVKDHITDSALRRLLLEAGKDIDSLMSLCRADITSKNDIKVKRYLA
                     NFDNVERKMQEVEERDRIRNFQPPVTGLQIMEVFDIKPGKVVGAIKEEIKEAVIEGKI
                     KNNFEEAYAYMIKIGKQNGLKVKKSGNIIEQ"
     gene            complement(46119..46313)
                     /locus_tag="VGA21_07420"
     CDS             complement(46119..46313)
                     /locus_tag="VGA21_07420"
                     /inference="COORDINATES: protein motif:HMM:NF023179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HEX9650372.1"
                     /translation="MEQKNQEFGYSYDITISELSVDSGGSHEISLVYQFEIINNRKRT
                     KAKGQIYSLPTFNSKSFWKR"
     gene            46547..48166
                     /locus_tag="VGA21_07425"
     CDS             46547..48166
                     /locus_tag="VGA21_07425"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009583025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEX9650373.1"
                     /translation="MASTKYIFVTGGVTSSLGKGIISASLGKLLHARGFSVTIQKFDP
                     YINIDPGTLNPYEHGECYVTDDGAETDLDLGHYERFLKVTTSQENNVTTGRIYNNVIT
                     KERKGDYLGKTVQVVPHITDEIRTNFLKLGESGKYDIVITEIGGCVGDIESLPFIEAV
                     RQTKWELGSTNVISIHLTLIPYLKAAKELKTKPTQHSVKQLLEAGIQPDILVCRSEYP
                     LPIDIRKKIALFCNVQLSDVIEALDAETIYDVPILMSKEKLAERALQKLKLKSGTEPD
                     LKDWKDFLGRHKNPTSEVNIALVGKYVELPDAYMSIAESFVHAGAENECRVKINYIHA
                     ESITKKKCKHLFKDLDGILVAPGFGERGINGKIEAVRYARENGIPFFGICLGMQCAVV
                     EFVRNVMKISDAASTEIEPATKHPVIDLMEDQKKITMKGGTMRLGAYKCNLVKGTKVY
                     SIYGKSKISERHRHRYEFNNKYLKQMEKKGMIASGINPETGLVEIIELKNHPWFIGTQ
                     YHPELKSTVLKPHPLFVKFVKAALDFHKTNF"
     gene            48177..49997
                     /gene="yidC"
                     /locus_tag="VGA21_07430"
     CDS             48177..49997
                     /gene="yidC"
                     /locus_tag="VGA21_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013452466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEX9650374.1"
                     /translation="MDRNQAIGLVLISLLLIAYFQFFAPEPKPQEEPAQTESVQPATP
                     EQSQTQEPDNSRIQDQETAPDDNQLANQFGIFAPAAKGKSEIITLENNQIEISFNSRG
                     ATPFQVWLKEFETASLEPLILMDSLSSQKSLVIHTGNKVIDLYKLYYQVEKSADNDTS
                     RVSFILNLGSGQTIRHIYSLGPSGYQLKYKLETSGLANLVDNINPELLWVNNLKHLEK
                     DITQSRIVTTVTYYTMRGDFDDLGERSADLEEETISEPVAWVGFKQHFFTEAIISKNG
                     FTRAVLKTQVDEADPAVVKKAFAGLSLDRAQFLGETNEFTYYFGPNNYQILKKVTEGF
                     SDNIYLGWPPINFVNKWLIIPIFNFLENYIGNYGIIIIILVLVIKILLAPLSYKSYVS
                     MAKTKVLKPELDEIKAKHPDDMQKAQQEQMKLYQQVGVNPLSGCIPLLLQMPILFAMF
                     YMFPNSIELRQEAFLWADDLSTYDSILSLPFNIPVYGDHVSLFTILMTASTILYTWSN
                     NQITTVQGPMKTFSYLMPIIFMFVLNSFPASLSFYYFVSNIVTFGQQALIRRFVDEDK
                     IKAILDENRLKNKGKKKSVFQLKLEEAMRTKEDAKKKKSK"
     gene            50052..50732
                     /locus_tag="VGA21_07435"
     CDS             50052..50732
                     /locus_tag="VGA21_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018477490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEX9650375.1"
                     /translation="MELVYYGQSCFQVSLSKAVILIDPFISTNPLSKNRDISNINPDY
                     ILLTHGHGDHTYDAEAIARNSNATIISNYEITTWYESKGIGSTHGMNIGGSYHFPFGR
                     LKMVNAIHSSSMPDGSYGGNAAGYVIETSEKTFYHAGDTSFFSDMKLIGDEFDLDFAF
                     LPVGDNFTMGIDDALNAASLLNTTTIIAMHFDTFPVIEIDHAACREKASAKKIDLIIP
                     KIGESLTV"
     gene            50742..51542
                     /locus_tag="VGA21_07440"
     CDS             50742..51542
                     /locus_tag="VGA21_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010853315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX9650376.1"
                     /translation="MGKIIAIANQKGGVGKTTSAINLAASLAALEYKTLIVDADPQAN
                     STSGIGFDPKEVKTSIYECMINEVSASHAVIATNLNYLYLLPSHIDLVGAEIEMVNLD
                     NREEKMRHSLLPIAPDYDFIIIDCSPSLGLITINALTAADSVIIPVQCEYFALEGLGK
                     LLNTIKIIQSRLNTKLQIEGILLTMYDTRLNLSNQVVEEVRKHFKSMVFDTIIPRNVK
                     LSEAPSFGLPVISHDVESRGSICYLNLAQEIINKNGYAPVTNGKQKVQ"
     gene            51520..52422
                     /locus_tag="VGA21_07445"
     CDS             51520..52422
                     /locus_tag="VGA21_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009183716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEX9650377.1"
                     /translation="MANKKSSKRTALGRGLGALLSDSSSFEADIPRKAPQLAGKSIDE
                     IQIEKIKTNPFQPRTDFDKDSLNALAESIKNQGIIQPITVREMSDGTFQLISGERRLQ
                     ASRISGLDTIPAFVRKANDTQMLEMALIENTQREDLNAMEIALSYQRLLDECNLNQEE
                     LAQRVSKDRSTITNYLRLLKLPPDIQAGLRDKKISMGHARALITINNVEAQLLIYKKI
                     ISDDLSVRKVEELARLLNKRSDSKESKKGKSKDGDIVRIESKLSSFFGTKIEIDHKGK
                     SGTIKIPYLSADDLERILELLNVR"
     gene            52412..52987
                     /locus_tag="VGA21_07450"
     CDS             52412..52987
                     /locus_tag="VGA21_07450"
                     /inference="COORDINATES: protein motif:HMM:NF040144.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5683 domain-containing protein"
                     /protein_id="HEX9650378.1"
                     /translation="MYGKPFVILIFIICFSQALRAQDTLVYVGDSVAINSLNIEQIKA
                     KRFFDPSRAALLSAVMPGLGQAYNKKYWKMPIVWGGLVLGISLANFYNERLQIFRVDH
                     FERLNGQPGTLMVSTRRLEQLIQRYRRDRDQTVIYTFMWYGLNIVDAHVDAYLKEFDV
                     NDELALKLEPYVDSSVPWQMNAGVSLVLKFK"
     gene            52984..53685
                     /gene="dapB"
                     /locus_tag="VGA21_07455"
     CDS             52984..53685
                     /gene="dapB"
                     /locus_tag="VGA21_07455"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009196321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEX9650379.1"
                     /translation="MKIILIGYGKMAKAVEVEAIKRNHTISEIIFHGDLNKLKKESGE
                     VVVEFTNPNSAFENIRECLNLGLPVISGTTGWLDKKSEIDAHCLKMDGTFFYASNFSI
                     GVNLFLKINKAVAGIMERKGYQISIEEIHHIHKKDAPSGTAISIANNILQNTTEYKEW
                     ALNQNKPYTIPIEAIREGEVPGTHKVSYSSDVDKIALTHEAYGREGFAKGVISAVEWI
                     SGKKGPMGMDDLLNI"
     gene            53752..54849
                     /gene="lepB"
                     /locus_tag="VGA21_07460"
     CDS             53752..54849
                     /gene="lepB"
                     /locus_tag="VGA21_07460"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEX9650380.1"
                     /translation="MDLKFWKKKDKDSEDKKPKSKVREWVDAIVFAVVAATIIRWLIL
                     EAFTIPTPSMEKSLLVGDFLFVSKVHYGTRTPKTPLQIPLTHQKIWGTNIPSYLDWIQ
                     LPQYRLPGFTNVKRGDVVVFNYPVEFNYPIDLKTNYIKRCVGASGDTLQIIDKLVFVN
                     GKEFENPQLMQFKYYVVSDDPINQRIFDRYDITDIFQGQGFYVVDTSPETAKELAALP
                     FIKEVRLYERKQGESDPGIFPDAARFPWNADFYGPIWVPKKGAAIQLTPDNITKYGDV
                     ILYYEHHDDARIEGGKVFIDGKEINSYTFKQNYYFMMGDNRHNSEDSRYWGFVPEDHV
                     VGKAFFIWLSLDPDKGILSKIRWRRFFNLIR"
     gene            complement(54852..55514)
                     /gene="ung"
                     /locus_tag="VGA21_07465"
     CDS             complement(54852..55514)
                     /gene="ung"
                     /locus_tag="VGA21_07465"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017732572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEX9650381.1"
                     /translation="MDVKIESSWKELLKGEFEKEYFNKLSDFVKDEYSRHIVYPKGRE
                     IFNSFNHCPFDNLRVVLLGQDPYHGPGQAHGLCFSVSKGVKMPPSLVNIFKEIRNDLG
                     KEIPADGNLERWADQGILLLNATLTVRAHSPGSHQNKGWEHFTDQVIKVVSERKEGVV
                     FLLWGSYAQKKGEVINKNRHLILSAPHPSPFSADRGFFGCNHFSKTNNYLASRNLKSI
                     DW"
     gene            55596..55982
                     /gene="apaG"
                     /locus_tag="VGA21_07470"
     CDS             55596..55982
                     /gene="apaG"
                     /locus_tag="VGA21_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012924864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HEX9650382.1"
                     /translation="MVTQITQGVKVSIETEYQPEYSSPSQYHYVFTYRVTIENNSPYT
                     LQLLRRKWQIIDVTSLRREVEGEGVVGQQPILEPGQIHQYVSGCNLRSGIGKMYGTYM
                     MEKVVDGLQVDVAIPAFTMIAPFRSN"
     gene            55989..56771
                     /locus_tag="VGA21_07475"
     CDS             55989..56771
                     /locus_tag="VGA21_07475"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX9650383.1"
                     /translation="MELGTLSRYIKYWLNAVEEHSLHSPYVYELYTQVIKSEYPDPEF
                     ERIEKIREALVNDNSIIKINDFGSGSKILHGNQRTISQIARTSISNPKLSKLLYRLIR
                     QQEAKEVVELGTSLGINTLYMYLGNEQIRLTTFEGDENIAKYAEHSFKQFGSENIQLR
                     TGELEKTLSQYLAFSSKIDFAFIDANHTFESTVRYFNKLAKKSHDESLFVIADIHWSS
                     GMERAWNEIKKSPYVTLTIDVFEAGLVFFKPDITKQHYILEF"
     gene            complement(56753..57349)
                     /locus_tag="VGA21_07480"
     CDS             complement(56753..57349)
                     /locus_tag="VGA21_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009183709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX9650384.1"
                     /translation="MRLDAAYWEYHYIHGTTGWDAGSITTPIKDYVDQLTNGDLRILI
                     PGAGNGYEAEYLFKSGFQNVFVLDFARTPLENFRKRVTEFPTENLIQTDFFTISGQFD
                     LIIEQTFFCSFDRNLRQRYAEKMFDLLIPGGKLVGVLFDSFLNADYPPFGGTLVEYKS
                     YFESLFAFRIFERCRNSIKPRQGTELFMILERNQNSKI"
     gene            57416..58321
                     /locus_tag="VGA21_07485"
     CDS             57416..58321
                     /locus_tag="VGA21_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009578198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEX9650385.1"
                     /translation="MTEEKKSTSKRNIIIIGIIAVIAIVNGVKWYLESEEREELQVYY
                     TNELSAAQTRFEEISVELDEKIAEIERLGGDIKELTEAKIEVEKERNQLQQTRTANRR
                     LIAELRDKTEGYAELLLAKDEEITKLKTVNTELLSENTTLKTEKNELSKSITELNQNK
                     EQLEEKVAVASRLEASNIAIYAVSDGGREREGSFRKRQIENLKIEFNIAKNDVAPIEG
                     KEILLRVIDSNNQVYFDVARGAGTFLIDGKEQFYTLKQDILFDNTSQKVTFLYEKGSE
                     YNAGDHTVEIYTDGYLMGKKAFTVK"
     gene            58398..58766
                     /gene="rpsF"
                     /locus_tag="VGA21_07490"
     CDS             58398..58766
                     /gene="rpsF"
                     /locus_tag="VGA21_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014022377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEX9650386.1"
                     /translation="MFKNYETVFILTPVLSEDQMKDTVLKFKKVLEDDGAKIINEEMW
                     GLKKLAYPVQHKSTGFYNLIEFEAGPQTIKKLDIEYRRDEKVMRFQTVSLDKHAIAYN
                     EKRRKGAFNKKSEVKEEPVL"
     gene            58763..59014
                     /gene="rpsR"
                     /locus_tag="VGA21_07495"
     CDS             58763..59014
                     /gene="rpsR"
                     /locus_tag="VGA21_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010610122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEX9650387.1"
                     /translation="MTLMNEPLNRVENRSKYCRFKKSGIKYIDYKNPDFLLKFVNDQG
                     KILPRRLTGTSLKFQKKVSQAVKRARHLALLPYVTDSLK"
     gene            59030..59473
                     /gene="rplI"
                     /locus_tag="VGA21_07500"
     CDS             59030..59473
                     /gene="rplI"
                     /locus_tag="VGA21_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009578192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEX9650388.1"
                     /translation="MRVILKDDIKGLGYKNDIVTVKGGYGRNYLIPNGFAIVANKSNS
                     KMIAENIRQAAHKAEKIKKDAEELAGKIGEMTIELGAKVGEKGKIFGSITAIQVSDAL
                     KDKGFNIDRKDITFKSQIKEVGDYEIILDLHKEVKHSITVKVAAE"
     gene            59554..60531
                     /locus_tag="VGA21_07505"
     CDS             59554..60531
                     /locus_tag="VGA21_07505"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF020459.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GSCFA domain-containing protein"
                     /protein_id="HEX9650389.1"
                     /translation="MFRTELNFPPSTEKIGLKDTIVSLGSCFADNHGERLILNKLSCL
                     VNPAGIIYNPYSICKVIDYALNEELPDQASYLQHDGVFSNFDFHSRISDTDGIKLQQK
                     ISHSLHQLQKNLVRAKWLIITFGTAVVYTRKDNKIIVANCHKVPASKFNKGMLSQKQI
                     LAVFMHTYQSIMAANPEIQILLTVSPVRHVRDTLEINSISKAILRITSDTLKTELPNI
                     HYFPSYEIMMDDLRDYRFYADDLIHPSHQAMNYIWEKFCKGFMDDALLDFINKWGEIR
                     KDLNHRPFHPETKSHLQFLTNTLEKLKQLSGEVNLEVEIAQLEKQLNDK"
     gene            60521..62536
                     /locus_tag="VGA21_07510"
     CDS             60521..62536
                     /locus_tag="VGA21_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017732561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEX9650390.1"
                     /translation="MTNKKANRLIKSNSPYLLQHAYNPVEWFPWDKEAHKISKETGKP
                     ILLSIGYSSCHWCHVMERESFENETIARAMNEKFICIKVDREERPDIDQIYMEAVQAM
                     GGHGGWPLTIFLTPELKPIYGGTYFPPENFLVLLEKVSSVYANNKAEMDSSANQIATA
                     LTGSELDKFRLRYNDEVSTLKLQKMYQNLSSKFDKEFGGMSRAPKFPMPGTWKFLSHF
                     AFIFKNNEALHHFFFTLDKISDGGIYDQAGGGFARYSTDRKWHVPHFEKMLYDNGQLM
                     SLYAIAYSLSQIDRYKEILYETLAWLSREMTHGSGGFYSALDADSEGVEGKFYVWKWE
                     EFERIANKNTNKFAAFFGVIPEGNWEGTNILLRNQGVPKEDQEEIEEFKSRLLKERNK
                     RIRPGLDDKILAGWNALMSSGLLDAYDATGDEVFLQHAVKNGTFLMNELFIDGKLFRS
                     WKDGKPGTAGFLEDYSCAIEMYLKLYQSTFTRKWLDFALQLVKIAYEKFYDPADGFFY
                     FSEQSSDLIARKKEILDNVIPASNSIMANALFKLGALMGDGKYIALAKKMTFSLIDLM
                     ESETEYTSNWALLYSYLVYPTSEVALVGKDFEKLGKAVRKEYFPNKVIAGTKAKSQIP
                     LLANRETSSKNSMIYVCQNHACQLPVTNPDEALEQMKDNLKIIKSHS"
     gene            62540..65041
                     /gene="priA"
                     /locus_tag="VGA21_07515"
     CDS             62540..65041
                     /gene="priA"
                     /locus_tag="VGA21_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009197273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEX9650391.1"
                     /translation="MKKFQLNMDAPEERETLFADIILPVPVPGLFTYRIPFLLNNQIS
                     IGSRVIVPFGSRKILTGVIRKIHNLPPAKYEAKLIHDVLEETPSINNFQFKLFEWIAS
                     YYMCTIGEVINVAIPSGLKLSSESLVQLHPEFDRDLSDYEFSEKEISVLNALSTDRSL
                     PYDRIHELTDTKNIHSIIKSLVRKDAILIYESIKEKYKPKKIKKIRLTGEHLDEKVLE
                     KLMKSLEKKGKQLDIVLMYLKTVDIFESPELNVLGMNKSDLPQSSSLQTLIKNKIFDE
                     FEVTVPRLETFSKDLKNVILNDNQQKVLDLILQEFESKDTVLLHGITGSGKTEIYIQL
                     IKEALDNGSQVLYLLPEIALTTQIVSRLRKVFGDKMGVYHSRFSDNERVEVWKGIQDG
                     KFSFIVGVRSSVFLPFSSLGLVIVDEEHELSYKQFDPAPRYNARDTAIVLAGFHKSKI
                     LLGSATPSIESYFNTKHRSYGLVTLSERFTNTPLPEIIPVNMIRQRKKKLVHGEFSQP
                     LFDAIGQALAAKEQVIIFQNRRGYAPHLVCEDCGWIPKCKNCTVSLTYHQYSKDLRCH
                     YCGYKENIVMQCPVCGSRSLHPVKYGTEKLEEDLRMLFQNAKVQRMDLDTTRKKKSYE
                     QIIEDFETGEIDILVGTQMVTKGLDFDGVTLVGILDIDRMMHFPDFRSYERTFQLATQ
                     VSGRAGRRDKKGLVIIQTNNTEHPLMNKIVNHNYKGMVNVEINERKRFRFPPFVRLIK
                     IIVRHDELIICEKAAKALSIRLIQELGLQRILGPAEPMISKIRNKYLREIYIKLERDK
                     VNLDKVKKNILEIGREIQNNKDFKKTSLIFDVDPY"
     gene            65041..65481
                     /locus_tag="VGA21_07520"
     CDS             65041..65481
                     /locus_tag="VGA21_07520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650392.1"
                     /translation="MKIFKIAAVIFFVFFLTACKEDDTPLDLTQYEPFVNIGFYDTLN
                     QPVETLIEQVQPLGHNPFTFPEPATTFRFPLDINTTMTKFDITISEETGTITVEYLRR
                     VENQINILRFIMEKIVVSAHSYDSVKIVCETPCISNETEIRIYR"
     gene            65453..66091
                     /locus_tag="VGA21_07525"
     CDS             65453..66091
                     /locus_tag="VGA21_07525"
                     /inference="COORDINATES: protein motif:HMM:NF039966.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6048 family protein"
                     /protein_id="HEX9650393.1"
                     /translation="MKLKSGFTGSILLLLIAAESFGQTDSIKNHFSVDLSIDYLKFAS
                     YLSDSQEKYEGELGIMFGRYRIAGEYGMAELTPKDLFDNADYYAKGNYWRIGFDFKLP
                     VNPKNNLYIGARYAATEFDESILIYYDSNTFNPYFEENMGLKANWYEAVISSESRIIA
                     QLYLGFKLRVRFMDSYDNPGLVEIYSIPGYGKSFDRSALAVNLFARYRITFW"
     gene            complement(66078..66866)
                     /gene="surE"
                     /locus_tag="VGA21_07530"
     CDS             complement(66078..66866)
                     /gene="surE"
                     /locus_tag="VGA21_07530"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009582780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HEX9650394.1"
                     /translation="MEKKRPLILISNDDGITSMGIRNLVNIMSEIGEVVVVAPDGPQS
                     GTGHAITVGNTLRLDPTDIFGDIPAYECSGTPADCVKLAKHFVLNDRLPDVVVSGINH
                     GSNTSISVLYSGTMSAAIEGAIEGFPSIGFSLCDYSSTADFSHCRRYVKEITEEVLRT
                     GLPKGVALNVNFPPKRNENIKGIKICRQARAKWQEEFDLRYDPNGRRYFWMAGNFVNF
                     DKGEDNDEWAIANNFVSIVPCMYDLTAHHGIAFLNEEWEIITRK"
     gene            complement(66921..67538)
                     /locus_tag="VGA21_07535"
     CDS             complement(66921..67538)
                     /locus_tag="VGA21_07535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650395.1"
                     /translation="MNKIAGLLIGFCLIGSLSPAQDFPSEFWHPGKMVLISGDTISGE
                     IKYNIETDLVQINGEGRLMTYSSRKIFYFEIFDVTIENYRQFYSIPYDLKSNYKTPTL
                     FEVIYEGKVTLLARESIVNETSHYNSYYWSGGTYTRQVLSYDYFILRKNGEIQYFSKK
                     YRDLLDLLKDRQREIKEYIRKNNLKTDEKSDLARIFAYYNALLDG"
     gene            complement(67541..68755)
                     /locus_tag="VGA21_07540"
     CDS             complement(67541..68755)
                     /locus_tag="VGA21_07540"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011584325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HEX9650396.1"
                     /translation="MSDIKLDKIEAAIEAIQNGEVIIVVDDEDRENEGDFICAAECVT
                     PDIINFMATHGRGLICAPLIEERCEELGLELMVGRNTALHQTPFTVSVDLLGHGCTTG
                     ISASDRSKTVRALIDPEIKPEDLGKPGHIFPLRAKKGGVLRRTGHTEAAIDFARLAGF
                     KPAGVLVEIMNEDGTMARLPDLRRVANKFSLKLVSIKDLIAYRLANETLIKKEIDVDM
                     PTTFGDFHLTAYRQTSTNEIHLALTKGSWKSDEPVLVRVHSSCATGDIFGSCRCDCGN
                     QLHKAMEIVEREGKGVVLYMNQEGRGIGLLNKLKAYKLQEEGLDTVEANLELGFKMDQ
                     RDYGVGAQILRDLGITKIRLISNNPTKRAGLIGYGLEIVENVPIEIAPNQHNEKYLTT
                     KRDKMGHTILKK"
     gene            complement(68822..>69901)
                     /locus_tag="VGA21_07545"
     CDS             complement(68822..>69901)
                     /locus_tag="VGA21_07545"
                     /inference="COORDINATES: protein motif:HMM:NF024554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HEX9650397.1"
                     /translation="GNVVSSSGTALTKEQITLIKRYTENVTILYDGDSAGLKASLRGL
                     DMLLEGGLNVRIVVLPDEDDPDSLVKKLGSNEMKELLASDSRDFISFKTELYAQEFAN
                     NPVKKAEVIREIVVSITKIPDPVKRAVYIKETAKKLDLDESILVAEQNKILIRERRDK
                     SKRLDSNQDVEPTQYPDIHNQDESISQDDKIRFQEREGIRLLITYGHSELEKEYKAYD
                     YFLSELDEIEFKTPVYKEILDIYKEQLAKGNLIDMQYLMTNGNEEVQREVVDLTTLRY
                     EVSPNWFGHKIPVPEESELLPNLFYHNILRLKFRLNGKLLRDKLKNLKDEPDPGKQEN
                     LQKDIARLKSAEREIGNLLGIVVND"
BASE COUNT        21897 a        13837 c        13746 g        20421 t
ORIGIN      
        1 acaattacgc tgtctttctc cagtggaatt tgagtttctt taaagtatat ctgccccact
       61 gcaaattcat caaattccaa tgaaattatt tgatcgggat tagtaaggtt caccgccttt
      121 gcctcatctt ttgtatagaa cctgacaaat tcattaacct ccgtgttatg caaatattca
      181 attatcttat tttcaaaaaa ttcagcactt acctcaagat ttctggcaac agccggaatg
      241 ggctctatca caacttttaa tgtcgccatc cattttgcct cttcaatttt ctctgctaca
      301 tctttgtatc cgcttacaaa ttggttggcg gttaaaaata accggtaagc agactttgca
      361 tcttcccggg ttcctttatt caactctgca aggccggctg agtaattctc ttcagcagca
      421 ttattcttcg caatctcaag ttctgaataa taatttttag gagaagggat aattcttaat
      481 gcaccgggag ctcgcattat ttcttcatac attatattaa tggacgtata attctgaaca
      541 attccgctcc acttaaaccg gtcattaccg gcttgtaaat tttttacctg catttcaaac
      601 caattcaagg caaaaggata tgcctgcttc aatgtttcgg aagatttctt gtgagttggg
      661 tttgatcgaa gtctgtctac agatttaatt actgcttcat agtagtcacc tcgctccaga
      721 gccttcttcc ctgaagtgca tgagccgacc accagcaaaa tgaaaaaata gtttaatgcc
      781 ttccttttca tagcatggtt gtttggtaac aaagttcaaa aaaaatagct ttaaattata
      841 acccggtgat aataacttgg tgcaggcaaa aatatttaca aaaactgtgc tggtttgatt
      901 cctcatattc tatataattg ataatctgat aattatgctg ggaaaagtag tgcagcctgt
      961 ttcacttttc tgtttagccc aggtaaaatc ggcgcctgaa ttttgcttat ttggattaaa
     1021 ttgccgccaa aattaagtta agtgtttaaa aagcttcagt ttcttgggtt tgtctttctg
     1081 ctgcttggcc atatagctat ttcacaaagc agagattctt atgtaataat gatttctatc
     1141 gatggaatgc ggaatgatta tacagagatt catgatgcaa taaatcttaa acaatttgca
     1201 tcaagtggag ctatagcaaa gtcgatgcta ccctcattcc caagtaagac atttccaaat
     1261 cattatacgt tggttacggg aatgtaccct gaacatcatg ggataattgc gaacactttt
     1321 tatgatcccg catcaaagat gacttatcgc ggtagtaatc cgaacaccgt tcaggacgga
     1381 aaatggtatg gtggtacacc actttgggtt gtggcagaac aacagaatat taaaacaggg
     1441 tgttatttct ggattggatc agaggcagaa attcaggatg tgcgtccatc ctattataag
     1501 tcgtacgatg aaaatatccc ggaaacaaag cgagttgaac aagttgtgga ctggttgaaa
     1561 ttaccggaaa gcgacaggcc acgatttatt actctttatt ttgaattggt tgatgatgca
     1621 ggccacaatt ttggaccact agcaccagag acaaaatcca cggtgcaaca gattgattca
     1681 attatcggtc agttaaatga ccaggtggca aaattaaatt taccggtgaa ttttgttttg
     1741 gtttcagatc atggaatgat acaggttgat caggagaatc caattgatat tacgggtatt
     1801 ttacctaaag aaggtgtaac tgttgtgcct ggagatacat tcatcctttt gcatagtgaa
     1861 gatgaaaacc ttattgatga aacttacaat aacctgaaat tatttgcgcg ggattttgat
     1921 gtgtataaaa aaggttttct gccagataat tttcatttta attcaactcc attggctggg
     1981 gatctaactt taattgcacg ggcaccaaaa gtctttggca ttatgggctc ggctattaag
     2041 ccgggagctc atgggtataa ctcaacgata cctgagatgg gaggatcatt tatagcatgg
     2101 ggccctgata ttaagaaaga tattaccatt gaaccttttg agaatattca tgtatttcca
     2161 ttgatcgcca gaattctgaa tctggatatt agttctttgg aaatagatgg gcgtttggaa
     2221 gtgcttaaag aaatcctgaa ggaagattga taatttactt ctttcctttc cactttaact
     2281 ggtaaagatc cagccttctg tctttcagag tttgcaccga accttcttta ttcagttcat
     2341 tcaaaaggtc cagattcacg tctgcaatta ccatcatctc actattaggc gttgcttcgg
     2401 caacaaccga attgttcggg aaatagaagt ctgatggaga atagacagcg gactcggcat
     2461 attgaatatc catattcctg actcttggca ggttacccac acaccctgcc attgccacat
     2521 aacattcatt ttcaatagcc ctcgctttgg aacaactttt cactctgtta aatccgttcg
     2581 gagtatcagt tagaaaggga acaaaaagta tctgcattcc ctggtcggcc aggatgcggg
     2641 gcaattccgg aaattccaca tcatagcaaa tgagaatacc aattttcccg gaatcggtat
     2701 caaaaatttt gagtgaatta ccacctgtca gtccccagaa ctttacttcc gatggagtta
     2761 tgtgcaactt cttttgataa tcccaggttc catctcttcg aaataagtag gcaacattat
     2821 aaagcttacc ccgttccaac aaaggcatac ttcccccaat aatatttgta ttgtaactca
     2881 ccgccaattc tctaaaagta tcgcacattt ttggggtata gcctgccagt tttctgatgg
     2941 cttctgattc tgcaagatta ttatatccgg ccatcaatgg agcgttaaaa aactccggga
     3001 acaaaaggaa gtcggattga taaccactaa cagcatcaac ataaaactca accatcccga
     3061 ctaattcctc aaatccggtt acggttctca tctgccattg aaccagaccg attcttacgg
     3121 ttgatttctt tggacttaac agttcttcct tctcttcata ataaagatta ttccactcta
     3181 ataatgtggc gaaagcctgc gactgattat cctggggaag gtaattacgt agaattttct
     3241 ttacatgaaa atcattggaa atctgaaaag ttaaaatcgg atcgtagatc tccttcattt
     3301 taactttctc gatatattct ttgggagtta attgatccgc gtacttatca taattaggaa
     3361 ttctgcctcc tgctattatt gatcgtaaat ttaaccgctc acagagctcc ttccgggcat
     3421 catacaacct gcgcgcaagt cgcattcccc tgaattcagg atgcacaaaa acatctattc
     3481 catatagtac atcacctttt ggatcatgcg tttccattga ataattatct gtgacttcat
     3541 aataagtgtg attatctcca aatttatcgt aatcaactat tagtgataaa gcaactccca
     3601 ccactttcgc attaacctcc acgcagagtt gcccttcagg aaaaattgaa ataagcttcc
     3661 tcatggcttc cttagaccac ggctgcccac ccattttttt ataagcaagc aacatagctt
     3721 cagaaagatc tttgtaatca ctaatcttta aattccttac aactactttc aggtcaatgg
     3781 gggtattttg tgtaatctcc atagcagaat agcaaaacgg gaaatgttta ggctaactct
     3841 actaagttac taaaaatacc tgattattcc gtgcggagat ttccaattgg attcgataac
     3901 gcaattttaa ttgcctggta ggcaatcgta aacagacaaa tcaaaattgc aattgacccc
     3961 ccggcaataa aaatataggg ttctaatcct attttatatt cgaaggtttt taaccaattg
     4021 gaaataaaat aaatggtgat gggtgcagca attaatgttg aggccagggt gagaaagagg
     4081 tacgatctgc ccaatgaaaa aatcaggttt ccaagagtcg cgcccaaaac ttttctgata
     4141 ctaatttctt tcattcgtgc tgtcatggtt agagcagata atccaaacag acccagtgaa
     4201 ccaattacta tagaaagtat agttgcactg gtaacgattt ggcccaggtt tccttcttgc
     4261 tgatattgct ccttaatagc ttcatcaatg aagctgtaat tgaacggatc atcagggtat
     4321 atctctttcc acatgttttg gactgcaatt aacccgtccc caatttcacc cgctcttatg
     4381 cgcaatgcaa ttttcggatt aaaagaagag tttacattta caccagtagc cccgctcata
     4441 acaatagcag agttcattgt taatactaat ggctctatta tagtatgtaa ggaagaaaaa
     4501 ttaaaatctt taactactcc aataatctcg tggtcggcaa atccttcatt agggattctt
     4561 ttgccaatcg ggttttccaa attgaactca tttacaaatg tctcatttac gattatgctc
     4621 ctccgattat ctgattcatt ttcaaaaatg aaatttcttc cttcagtaat ttccatttgc
     4681 attaagggaa taaagtttgc gtccacggta ttcacaaaga aatcatgctg agtttgatcc
     4741 tgatcattat aacttaattt catccaaccg gcatcaccaa aactttgcgt agatacagct
     4801 acttcttcaa cctgtggcaa gccccgcaac gtagcagtta ttctttcaga tttatcgaag
     4861 cccttcgtaa ttgtttcctg aagtcccctt gcatcaggca cagttagtgg aatcattatt
     4921 acctgttcct tgttatagcc taaattcttg ttttgcagaa attgaagctg acgtttcatg
     4981 aatattgtcg aagtaattag aaaaatagac agcacgagtt gaacaaccag cattcctttt
     5041 cgcgcaccct gtttcccgga tccaataccc aggctgccct taagaatttt ggccggaaca
     5101 aatccggaca taaccagggc gggatagata ccggctataa caccaactcc aattgccagc
     5161 cctgcaaaca tgagtaggtt caaggcgcta aaatcaaatt caagattttt cccggataat
     5221 tcattaaata ttggcaacat ccagtaagaa acggcaagtc caataaccag ggaaattatt
     5281 gccagccaga ttgcttcacc caaaaactgg cctactaact gaaagcgcat ggcacccact
     5341 acttttttaa ttccaatttc ccgggatctg gcaaatgacc tacccaggga aagattcaca
     5401 aaattaacac acgccataac caggataagc aaagcgattg cggaaagaat taacgtgtac
     5461 ttcggatcac tgagttgagc aattccctgg ggcatgcttg tgttcaggtg aatatctttc
     5521 agctgttgca gtccaacatc aaattttctt ccctccagtt gctcaccgat agccgtttta
     5581 accatatcag gaaatttacg ctccagactt ttagcatccg cgccatcact caataaaaca
     5641 taagattctg ccgctatcat gttccatgat gtaatcattt gtggcggaaa aatatcttta
     5701 gcattttctt catgaacaag actggtaaat tgaagtgaag aattgcttgg tacatcttca
     5761 attacggctg atacaataaa tgcctttttg ccaggcccaa caggtatcag gagagttttg
     5821 tcaatcgcgg attccttacc aaaatatttt ctggccattg attctgtcaa aacaattttt
     5881 gaagaattcg ataacgccga ttccaatgat ccttcaacag gtacaaaatc aaaaatttga
     5941 aagaaaccag gtgaggcaat attaatttcc tcactgaacg atttctcttc atattctatc
     6001 tccgcctgga atgaaatcca gattgtagtt gcctccactt ccggataatt ctgtttcatg
     6061 gttgcaccaa taaccatggg agtaaccgtg ttggttacca tacttccgtc tcttctttcc
     6121 gctgtataaa aagtgcgata tatgtttgaa gcctggctat gaaagttatc aaatgtcaat
     6181 tcatctttta caaacaggat aatcagaatc gcgcaagcta tccccattga caagcctaag
     6241 atattaatgg aggtatgaat ctttgttcga aaaaggtttc taaaagtaat tttcagatag
     6301 tggttaatca tgctttataa attaaattaa ccaatagtac ggtaattgaa ggttaccgga
     6361 ttcaaaatct gagttaataa ttgttaataa tcgggttaat ggatctccac taccgaaatt
     6421 accggaaggt gatcggaaag gtttcggtca ccaattccgg gagttaagat ttgaaagaaa
     6481 aggcaatcaa aattttttat taaaatgtaa tcaatcctgg gaccggtatc ataattcatg
     6541 ttggtgaatc ctgaaaatgt taaattgggt cctttctttt ctttgcaaag gaatgtatcc
     6601 attaattctt cggagaaaat cttaaccggt gaagaatccg gttcagcgtt cagatccccc
     6661 atcagaatga gtggttttcc tggatatctt ttatcaatta tatcaagcag aagtttgctg
     6721 ctttccagtc ttgccagttc accacggtga tcgtaatgag tattaataac caccacctgc
     6781 ctttctgttt ccttaacccg gagtacaacc acggtagcta ttctctccag ggcggcatcc
     6841 catccaatac caggtttttc cggcgtttca gataaccaaa tggtttcctg atcaagcaaa
     6901 tcaaattttt ccttcttaaa aaataacggg ctgaactcac ctttagaaat accgtcttct
     6961 cttccaacac cgagagaatg gtattgatca aggccggttt tgagaaactc taattgtcta
     7021 ttcagtactt cctggaagcc cattatgtca gggttttgtt ccagtaaaaa ctttaccatc
     7081 tcgcttctgc ggtgtaccca ggcattttct ccatcctccg gattatcata ccggatatta
     7141 taagttaata ctttaattga ttgcgaaaat gacaaagccg gcatagtcat tatgataatt
     7201 attggaaaga ggtttatcaa ccctgctgaa aagtttatct ttgacctata tttcaaatca
     7261 gatagattta ttaaaagaaa acaaccatta catgaaatcc atattgataa tcattggttt
     7321 aataattgta ccggcaattt cttattcaca ggaagttgaa aatactaata aaaatctgcg
     7381 cgaacaatat caggacctta aggagactac cgaaacttat aatcaataca aagttatccc
     7441 tgaaaacagg ttagatggct ggtttaaaac aatccaggat tcaatatcag ataaaagcaa
     7501 cagaattaat agtttactga cttcaacaac tgcattaaag gatacaattt ccgatttaag
     7561 aaatgagaag agtcaaatgc aaacagagct tgatgaaggg gattttgaaa aaagtcatat
     7621 tggggttctt ggagtcaact tcactaaggc agcttatatt ggcataacct tcatcctgat
     7681 aatcgggtta tccatcttga tcattttatt cgtaataaaa gtaatcgata ccggtaaaac
     7741 tacgcgggat acaataaaac tgcatgatac gctgacggcg gagtttgaag aatacaaaag
     7801 acgtgccctg gagaagcaaa cgaaacttgc ccgcgatctg caaactgaaa gaaacaggat
     7861 agatgaaata agacgtaagc gctgattatc aaacggaaat atttctttca aattcctcaa
     7921 catcaaattt tatgtcatcc tctcccacct ggtcgtatgg atcttcaagg tgcacctgaa
     7981 tattatccag actcaccagg ataaaactaa aaagcaccgg cataatgtat tcgagtccaa
     8041 cactgtattc ctgggaagca aagacaaaat aaggtccgta aattatagga aatgtataaa
     8101 taaagacttt gctgtaggct ctcaaagtaa taggagttct gtaatgcaaa atgttcttca
     8161 tgttctccag cgcaatactg attttagaaa tgtattgact tacccttgag acttcacctg
     8221 gttgcaaccc atgcttccga aaatcttgca acaaggtact aagcccggat atttcatcaa
     8281 aaattttctt ctcaaattta tcactatctt tccgcttact cataaataaa tctctaaccg
     8341 agccataaat ctgaatcaag ttgtcttgga tttttatatg gaactccgtt gagcctgcgt
     8401 caacccagtc tctggaagca tgatagatgg caaggcaatg tcccttaaaa tctgccaaat
     8461 agccaagagc ccgctcacgt cttttatatg cactgtcaat agaaaaaact accggaaata
     8521 caattgctaa ccccacaatg tttaatggaa agtctgccac aatttgatag tactcacaca
     8581 accatgtgct taccagggat aagatggtga ctataaatgt cttgtaatta attatcaggt
     8641 aaatgctacg aaggagtttc atattattgg aatgttaagg caaaataaaa aaaaatcccc
     8701 gttttactaa tgaattgcaa tatttggatt attccagacc ttatttccct gggttttttt
     8761 gagattcctg cctggtgttt agcccatcta tcaaaatctg tgtaatgtct aaagcattgg
     8821 cagcgtagag cacattagga tttcccttag tataagttaa tacaattttt atgtcttgct
     8881 cagtgccata tttacccaga aagttggcca cactatcgta gagctgggtg ttcaccctgt
     8941 tttgttctcg tgccatatcc tgagcaaggg tttgtgagta ttgctgaaga ttttgctgtt
     9001 tcttcattaa attctcctcg atatttctcg cctgacccat ggtaagatta cccgcattct
     9061 gctgataatc atcaatctcg gcttgcaatc ccttttcacg gttaagatat tccgcttgaa
     9121 acttatcccg ctttacttcc aattgcttca tcagcacatc agataatgaa tagtatttga
     9181 gcagggtatc ggtatttaca aacgcgattt tcatatcacc aataactgat tcatttatcg
     9241 gctctgcttg ctctttaatt tcttcttttt gaaattgaaa aagatagagc aaaccaacag
     9301 ctatcaggag caatacattt atgatgaggg agatattttt catggtttag ttattaaagg
     9361 tgtaaatata tgccaaaaag gaattacctg aagacaggct aaatcaggtc ctctttgtca
     9421 acatcataat tatatgttgg agaggatccg atttttttct ctttcttcca aacaatggtg
     9481 taagactgcc cggaaggtgc cggttcatcg ggtaccattt cgtgttcaga agtttcatct
     9541 tccagaattg tatcaactga tgaacgtgag ttcttgaaaa aataagcaca aaacatcccc
     9601 gacaaagcac ccattaggtg agattcccat gagatagctt cgttaacagg aaagatcccg
     9661 taaatcatat ttccgccata aataaataac aacacaagag agaccgcaag tgtttttgca
     9721 tcccggcgca gaagaccgct aaaaaaaaga aaagcaagaa ggccgtaaac taatccgctg
     9781 gaaccaatat gataagcgtc gcgcgcaacc acccaaaccc aaaatcctgt cattatatat
     9841 ataattagta tcacttcaaa agcaatcttg tgataaaagt agaacaagcc gacgccaaga
     9901 ataataagag gaaaagtatt tgaaaccagg tgaaaaaagt ctccatgaat taatggaccg
     9961 gtaatgatac ctattgtgcc ttttagcgtt ctgggcaaaa taccgaactc tctcaggtcc
    10021 atgctgaagg ctacttcaaa ggcctgaaca gtccaaagaa gcagaatgaa agtgattgaa
    10081 atccaaatac ctcgtctaaa tcttatggca tcattcgaca tcgaattcaa tttactaaaa
    10141 aaataactcc tgggccagtg tgaagggtat tggcatgagc ttcaatcata taattaattc
    10201 ccctccttat tttaaggagg ggaaaggggt ggttataacc ccctgtagtc cccctcaaaa
    10261 aaagggggac aatttctagg cctgcggtta aatatgaaat ttgttaaatc aatttaagaa
    10321 ttctcctaaa aataaaatct ggttttacta tgaaagtaaa atcacttgga aattaggttc
    10381 aaaggaagta actttttatt gcacataatt gtatatgcat ttacatcgtc atgaaggttc
    10441 cgtggaaaag aaaggattta ggctttggct tgccattatt ctaaacgtcg ggataacttt
    10501 tgctcaggtg gtaggcggaa tatttgccgg gagtttggcc cttctatccg acgcccttca
    10561 taatttaagt gatgtactcg ctctatccat tagccttttt gcacgtaaac tggtgcataa
    10621 aaaaagttca tcttccaaaa cctttggatt taagcgcgcc gagatcctgg cagcattcat
    10681 taatgcagca tcattggttg ttgtcagcgc ttacctaatc atggaggcaa taaagcgatt
    10741 gatccaaagt cctgatgaaa tcgtggatgg caatcttgtg atgataatgg ctggagtagg
    10801 tatagtgggg aatgcattga gcgtggctat tctccataag gaatcaggtg aaaatatgaa
    10861 tatgagatca gcctatctcc acctgatatc cgatatgtta ttttcagttg cagttttagc
    10921 aggcggattc gtaattgtaa aatttggaat atactgggta gacccggcgc tttctattgt
    10981 aatcgcggtg tatttaattt ttattagctg gaaacttttt tataaaagtc taaccgtgct
    11041 gatgcaattt gccccggggg aaattgattt gacccaggta gctgctgtta tttcaaagta
    11101 cccagagatt gataatattc accacatcca tgcctgggaa cttaacgagg atgaaattca
    11161 ttttgaagct catattcaaa taaaagaaaa tattaccatt gacaaggttt gtgaattgct
    11221 gcagagaatc gaagaagaac ttcagagtga attcggttta acgcacatta ctttacaacc
    11281 cgaatttaac cgagattgcg gagaagagtt atttgtagaa gaacatcatc atcactcttc
    11341 ctgataccac cccgaataca taatgtaatt tttcgaagtg cgttcgaata catttccaag
    11401 gtctccaaca gttttcaatt ttttggcagg aactcctgcg tatatatacc cggattctac
    11461 aattgcattc atcagtacaa cagcacctgc cgctacaatt gatcccgatt gcaccacagc
    11521 gtgatccatt acgatagctc ccatcccgat taagacatta tcttctattg tacaaccatg
    11581 aacgatggca ttgtgcccaa tactaacatt gctgccgata actgtctttg ctttttgata
    11641 agtaccgtgt attacggctc catcctggac attcgtatta ttacctatca ttatagaatt
    11701 aacatcacct cttaaaacag cattaaacca aacactgcaa ttatctccta tggtcacttc
    11761 tccggttatt gttgcatttt cagccaaata acaattattg ccaatagtgg gcttaatacc
    11821 tcttacattt ttaattaaag ccattactcc tgttcctttt aattatcaaa tgtaatagca
    11881 aatgtaccaa ttatggcctg agcctttact tttagggaac attccttatc tacactgcca
    11941 ttttgtcatt acatctcctt gcgctacctt tgtatttcga ttttgaacaa tggaacctgt
    12001 tattaaggat atcaatattt tagatgataa tcgggaggaa tcctgtgaag ttgttaaagt
    12061 ctctgaagag aataacgcag gcaatacacg caaattatat attgaaagct acggctgcca
    12121 gatgaatttc tccgacagtg aaatagtaag ttcaataatg gccaaagagg gtttcgatac
    12181 aaccagcagc ctggaaaatg cggatgtgat tttcataaat acctgctcga ttagggataa
    12241 ggccgaacag accatcagaa gcagattagc attttttaat agcctcaaga aacaaaaacc
    12301 agaattgcag ataggtgtac tcggctgcat ggctgaaaga ttaaaggcga agcttttgga
    12361 agaagagcgc attgtagatt tagtggccgg ccccgatgct taccgggatt tacccaattt
    12421 ggtcaggcag gttgatgacg gtcaaaaagc ggtaaataca tttctttcaa gagaagaaac
    12481 ctatgccgac atatccccag tgcggctgaa tagcaacgga gttactgctt tcatctccat
    12541 catgagaggc tgcgataata tgtgctcgtt ttgcgttgtg ccatttacca ggggtaggga
    12601 gcgcagccgc gatccggaaa caatagtcaa tgaagcttta aatctctaca ataacgggta
    12661 tagagaagtg accttgcttg gacaaaatgt tgattcctac cggtggggag ataaagaaaa
    12721 tccgcaggta aactttgcac aacttctcgg gaaaattgcc gcggtttctc cagaacttag
    12781 ggttcgattt tcaacctccc atccgaaaga tattaccgat gaggtgctgg aaacaatagc
    12841 ccggtatgaa aatatttgca actacataca cttgcctgct caaagtggaa gtacgcgggt
    12901 actggaatta atgaatagaa catataccag agactggtat cttgaccgca ttggtgctat
    12961 aaggagaatt gttggaaatg aatgtggaat ttcttccgac ttcattgcag gtttctgcac
    13021 cgaaacagag gcagaccatg agtacacctt aagcttaatg gatgaagtca gatatgactt
    13081 ctcatacatg ttcgtgtatt ctgaaagacc cggaaccctg gcagaacgaa aatttaaaga
    13141 cgatgttaaa cctgacatca agcagagaag attgagagaa attattgata agcaacaaga
    13201 acacagtctt aagagaaatc agcgtgagtt agataaagtt cataaggttt tgattgaggg
    13261 ttattccaag agatccgaag agtatatgca gggaagaaat tcagcaaata aggtagtggt
    13321 ttttccacgg gagaattata agaaaggtga atatgttaac gttagggtga ctgattgtac
    13381 agccgcaacc ttgatgggaa aagcagtgca agatgattaa tgataaggaa atacaatcca
    13441 ttaaacaacg atttgggatt ataggaaact caccctccct gaatcatgcc attgctgtag
    13501 ccatgcaagt agcgccaact gatatgaccg ttttgattac gggggaaagt ggcagtggaa
    13561 aagagtcctt ttccaaaatt attcatgcac ttagcccgcg taagcatgga cagtttatcg
    13621 ctattaattg tggagctatt ccggaaggaa ccattgactc tgagcttttt ggccacgaaa
    13681 aaggatcttt tacgggagct tatgaagcca gaaaaggata ctttgaagta acctcaggtg
    13741 gtacaatctt tctggatgaa attgcggaga tgccgctcaa tactcaggcc agattactga
    13801 gggttttgga atacggggaa tttataaagg taggctcctc caaagttttg aaaactgatg
    13861 ttagggtagt tgtggcaacc aacgttaacc tgtcagacaa aattaaaaaa ggtaaattca
    13921 gggaagatct gttttataga cttaatacgg taccaatcgc agttccatcc ctgaaggaaa
    13981 gaggtgatga tgtagagttg atctttcgaa aattcgctgc cgatttttca gaaaaatata
    14041 aagtgaaacc aatttcacta accgaagaag ccaaacagta cttattggga ttcccattcc
    14101 ccggtaatat ccgtcaattg aagaatttag tcgagcagat atctgtattg gaaaccgacc
    14161 gcgaaatttc cagggaaaaa ttaatgaaat accttccttc gtccagttct tcccttccaa
    14221 tgctatttga taaatcgact attaataacg aaggaatgac ggaacgggat ttgctataca
    14281 aagtcttgtt tgatttgaaa agggacgtta atgatttgaa aaagttagtg tatagcaaca
    14341 ttagcggaga tgtatcttcg gcaaaactca taaaagatca ccaggaacta ttcgatagta
    14401 ttgaagaaaa acctgcacgt cagaaagaac agtcaccggt tttattgaat cttgattcca
    14461 acgaagccaa atcttacgaa ttggaaaaag tggaagacat catgcatgaa acggaagagg
    14521 acgaagaact atccctggat aaaaaagaaa aggaaatgat aattaaggct ttgcgcaaga
    14581 acaataacaa aagaaaatat gccgcgcatg atcttggaat ctcagaacga actttgtaca
    14641 ggaaaataaa gcagtatgaa attgaggagt agattttttt tgattttccc ctttattctt
    14701 acgggttgtg gcatttattc ctttaccgga actactcttc cttcaagtat aagaacattt
    14761 tcagtgcagg aattttttaa tgacactgat ggaggaccac caaatcttgg attaacctta
    14821 accaatgaaa tgagggatta ttttcaatca aattcctcgt taacccttgt tcaggaggaa
    14881 ggagatttgc agtttgaagg cggtatcatt gagtacaggc ttacaccagt agctcctacc
    14941 aataccggca ccggtattaa caatattact tcttcttctg taaccaggct taccatagtg
    15001 gtacgtgccg cattcaccaa tacagttgat gaaacacaga gttttaacag gagttttaat
    15061 ttctatgcag attttaatag tgtaactgag actttaaatg atgcggaaaa tcggttaatt
    15121 gaaactattt ttgaacaaat aatactcgac atttttaatg caagcgtagc agtatggtag
    15181 gcaaccttaa aatttgttat attagtaatt ccgctatttt ataatttatc accttgaaca
    15241 aagataaatt catcacatta ttaagaaatt accggaatct ttccggtgaa gatctgaagg
    15301 agctttcctt actttcagat gaattcccgt ttagtcaggc attgcataac cttgttgcaa
    15361 aaggagcgat ggacatgaaa gccccggaag caaaaacaaa attaggcaca gcagctattt
    15421 actcacctga ccgtgccgca ttaaaaagat taattgaggg gtcagtgcag tttgtagctg
    15481 cagttcatga agaatctttg gacaggagca ttgagactcc ggccgccaca cccaaaacag
    15541 aaaccccacc ccctccacct gaacctgaaa aaattgataa gcctgttact caggataaag
    15601 ttccgcacac ggaaatgcct gccgctgaag ttaagacacc agagccggtt tccaaacctg
    15661 cttctgttca ggatttggtg aaagacccga ctcatcaagc caaggttgaa gcattaagaa
    15721 atgaaatttt ttccaatctg aaggaattgc aaaagaatat cgttgattca aataaggtct
    15781 ttgattttta tgataaaaag catgaaaacg cagatgcagc caaaaagaaa gcggttcagg
    15841 ataagggcag agagttaaaa gaattggagg aatcagccaa agccaaatta tcagggcggg
    15901 aaaaagctga ggacaaggaa ataaccatgg gcactgaggc agccccggta aaagaagctg
    15961 cacctgagac tccaacaaaa ccaaaatcag tcaggaaaaa aactatatct aaaccaaaaa
    16021 aaactaaatc gaaaagcccg gcaaatagaa cgaacaaaag tgccattaaa aagaaaccgg
    16081 caactggtaa aaagaagact tctaaaaaag ctgccggtaa aactactaaa gttagcggca
    16141 gaaaaactgc taagaaaaaa accatttcca ggaaagcccc ggtgaagaag gccgcgtcaa
    16201 aaaagaaggc tgcaacgcgg aaaaagctga agaaagcagt tggaaaatca accaaaacaa
    16261 ctggttccag ccgcaaaaaa acaactgcca gatcttctaa aaagacggta aaaaaaaaag
    16321 tgaaccgcac ccggaaataa gagattacgc cattgaacta tcttataagc cggaaaaaag
    16381 caattacaga tcaatcaatt taagtcaaag ggaaattatt gatcgtttta taagacgcaa
    16441 gcaccctcgg attgatcctt caaggatgtc agaaataagg gaagacctca ctgccaatat
    16501 cactaaaatt agcgattcgc tgcttaccga aaatctggct gagatatttc ttcagcaggg
    16561 gaaaagcgat aaggccattg agatttacaa gaaattaatt tggaagtttc ctcaaaaaaa
    16621 ggcgtacttt gcatctcaaa ttgataaact aagtagaagg taatgttcac ccttgttatt
    16681 tcattgattg taattgtttg tgtgctgctg gtattaatta tcctggccca aaacccaaaa
    16741 ggcggcggat tatcgagtca gtttggagga agtggggcta gcaatttgat aggtgttaag
    16801 aaaacaggag acctattgga aaggctaacc tgggggtttg ccattacgct ctttgtattg
    16861 agtatggcta caaatttcat tataccatca aacacgggaa ccgggttgaa cagcccgaac
    16921 attgacaggg ctgaggaaca gcaacaaact cctgaacttg acctggaagg gattcaggaa
    16981 gaatcccagc cttccgataa ttcttccctg gaagatttgc ttgaaggcga ttcaaccaaa
    17041 taattacttt aagactgcaa ctttcctgtt gaagaaatat gccttatcag caatcttttt
    17101 gctattggca tcagagtagc atcataagga aacactgtct tcgaaactat gagatatgtt
    17161 gcagggttag ggagtaactt gaattttctg attaattcca gtttctttct ttcaaaactt
    17221 gaatacaaag accgggtaaa agtttcgatc cactgcgtgg tgattcccct gaatttctca
    17281 tcagaatttt caaaaactga cattgcatat ttgtgtacat gagcttcgct gctggaatca
    17341 atgtacaaga acaaatagcc ttcatcaaca tacaatgggc aaagtccgat ggtggagatc
    17401 tcaaaattcg actcaataaa ttcataaatg tctcttcctt cctccaaagc ttgtttcatc
    17461 gttggcaaag caaattcaat tatgtcctga atttccttca ttacctcatc atctttaatg
    17521 atttttttgt atgaaagttc aagcttttcg aaatcagcct tcgaaattga ctgcggaaag
    17581 cttccataaa gcaactcctt attatcccgc aattgcatca gatttctgta atgaaatacc
    17641 aggtctgaaa gcccgggaaa catttccata cggctgaatc cgcttttcac ctcttttaaa
    17701 taggctaata agtaatattt cttgtattca aaatcaatca atccttcagt taaccaattc
    17761 ttgctaagtt ctttcataac gcagtccaat tagtcccagg taaaatataa aaatgattca
    17821 taagatcaaa aatcaattcc aatcaagctg ataaaatgtc aggtttactg tcatagagta
    17881 aaagaaaatc caaaatccgg ccaaaaactg acaaacctga cacaaaaggc ggtgattttc
    17941 taattggcat aggaaatgct cattgctgct cgaaatcgta tattttattt aacgttaaac
    18001 ttaaaaatag gtatcaaaat gtctaaagtc aaaattaagc cgcttgctga tcgagtattg
    18061 atagcagcat ctccagcaga ggagaaaaca gcttccggtc tgattatccc ggatacagcg
    18121 aaagaaaaac ctcaaaaagg aaaagttatc gctgttggaa tgggtaagaa agacgaaccc
    18181 atcactgtta aagtgggtga tactgtgcta tatggcaagt attccggcac tgaaatcaac
    18241 attgatggta aagattatct cataatgcgc gaatcagata ttttcgccat tatctaaact
    18301 attacctcat tagtttaatt aattaaccta aaaacattaa agaaaatggc aaaagaaatt
    18361 cactttgatt cagccgcgag agaaaatttg aagaagggtg ttgatacact cgcaaacgcg
    18421 gtcaaagtaa ccctgggacc taaaggtcga aatgtaatcc ttgataagaa gttcggagct
    18481 ccgacgatta ctaaagatgg ggttactgta gcaaaagata ttgagctcaa aaatcccctg
    18541 gaaaacatgg gagcacaatt agttaaggaa gtggcttcaa aaaccgcaga tgatgctggt
    18601 gacggaacta caactgcaac tgttttaacc caatctatct tcactcatgg tattaaaaac
    18661 gtcgctgccg gtgcaaatcc aatggatttg aaacatggta tcgacctggc agtagccgcg
    18721 gtagtggata gtttgcaaaa acaatcaaaa cccattaaaa acagcagtga aattactcag
    18781 gtggcttctg tttccgcaaa caacgacgaa gaaatcggtc agatgattgc ggatgcgatg
    18841 gacaaagttg ggaaagatgg ggttataact gttgaagagg cgaaaggaac tgaaactgaa
    18901 gttaagactg tggaaggtat gcagttcgac agaggttatc tttctcccta ctttgttacc
    18961 aatgccgata agatggaggc ggagttagaa tctccattca ttctcattta tgacaagaaa
    19021 atttcttcaa tgaaagaatt gcttcctgtt ttagagcaag tttctcaaac tggaaagcct
    19081 cttttgatta ttgcggaaga cgttgacgga gaagcactgg ctactcttgt tgtcaacaag
    19141 atccgaggag cattaagagt tgctgctgtt aaagccccgg gctttggcga caggagaaaa
    19201 gctatgctgg aagatattgc tgttcttact ggtggtaccg taatttctga agagagagga
    19261 tataaattgg aaaatgccgg actggattac ctgggttctg cagaaaagat taacattgac
    19321 aaagacaaca cgactattgt taatggtgcg ggaaaaaaga atgatatcaa ggcaaggata
    19381 agccagatta aggctcaggt tgaaactaca acttctgact acgacaagga aaaattgcaa
    19441 gaaagactgg ccaagctaag cggtggtgtg gctatcttgt atattggcgc ggcaacagaa
    19501 attgaaatga aggaaaagaa agacagggtt gatgatgcat tacatgcgac acgagctgca
    19561 gttcaggaag gtatagttgc cggaggtgga gttgcattcg ttagagctat caaagctttg
    19621 tcgaaactgg aaggaaggaa tgaggatcaa aataccggta taaatatcat caggttggca
    19681 cttgaatcac ctttgagaat cattgtggag aatgccggaa tggaaggttc tgtggttatt
    19741 caaaaggtaa aagaaggcaa ggatgatttt ggttacaatg cccgcgacaa caaatatgat
    19801 tatatgttta aagcaggtat tattgatcca accaaagtta ccaggctggc tctgcaaaat
    19861 gcggcttcaa ttgcagcttt attgttaact actgaagcag tagtttctga agtacctgaa
    19921 gaagaaaaag cgccggctat gcctcatggt ggaggaatgg gcggaatgat gtaaaatcat
    19981 ttgatgctta atgataaaag gctgcttcct gcatggatgc agcctttttt tatttttctt
    20041 taaactagaa tttattgaat taccaaaaca acttctgaag atctcagcac tttgattgtg
    20101 ctgccggtta ttattttgtg aaattctagt cagaaattca gcaaaatcta gtggttctca
    20161 aactccaaat catttctgat catcttatcc accagtaatc ccgattcttt catttcttca
    20221 tcatcagtct tgtaaatcca ggtgatgcgg atctttttct tatgcataat atttttttca
    20281 agaagacgca gtagccgtgc aatttccaga aggctctgaa cccgaactac ggaaaggtgt
    20341 attctaaatt ctatctcatc cggaaatgag ggaagatgcg tttcaagcca gtcatatacg
    20401 tcggaaaaga aattttcagg attttctgaa atacttttgc cttcaatttt catctttcct
    20461 gaaagtgagt cgaggtaaac tcccggattt atttctgtag caggtatatc taacttatca
    20521 tccaaccgaa cctcaattaa tcattattga acatttccca tccatttctt catcaaagga
    20581 gaaaagataa caaacaaaat tcccgagccg cagccaaaca acacaacggt cataaacagg
    20641 ctgggcattc cggaaacatt attggggtcg aattcaccgg caaacattcc ggcaatcaaa
    20701 ttgccaagag aagttgctac aaaccagata cccatcattt gtcctacgaa agctttgggt
    20761 gcaagcttgg tcgttgcact taatccaaca ggactcaatg ccaactcacc tgctgagtga
    20821 aggaaataag ttaaaaccag ccaggccatt ccggcttgca tgccttcaac aactcggtgc
    20881 gcggcaaaat acattaccag aaatccaaat cccatcagaa tcaatccaag tccgaatttc
    20941 agaggagtag ccggattaag gttttttgcc gcaagtttta cccataacgc gccaattata
    21001 ggcgcaaaaa ttacaatcat taatgggttt acattttgca gccatccggc aggcaattcc
    21061 cacccaaaaa gaaaacggtt ggtgtaatcc cgcgaaaaaa gattaagagt tgagccagct
    21121 tgttcaaacc cagcccaaaa cagggcagct ccaataaata aaaagaaaat aatcccaacc
    21181 ctccttttct cttctccatt cagaccgccc gcgataagta tatagagaaa ataaaataat
    21241 gtgatcgaaa cgataagcac tcctgcaccc gccgctatac ctcctgctga agttaagtca
    21301 ataacgttca cgtattgcaa cacaacaagg aaagcaatta gggcaattac caacccgatt
    21361 gccattccct tatttccggt aatctcaccg ccttcttctt gtctttcttt tgccttgggt
    21421 tttatgccaa gattcaacaa agtatcctgt gaaagtctga attggagcag accaaaaaac
    21481 atcccaaccg cggcaaggcc aaatccccag tgccaattaa ttttttcacc taccagggga
    21541 acaacaaact ggcccaatat ggaacccgta tttatcccca tataaaataa cgagaaacct
    21601 gcatcccgac gagcaccacc ttccggataa aggtcaccca ctattgtact gatattcggc
    21661 ttcaataatc ctgttccgca agccacgaat gcaaggcctg tagcaaaaat tggcacagat
    21721 cccggaatgg caaggattaa gtgtcccatc ataatcatta ttccaccata ccatatagcc
    21781 ttccgctgac caaaaatatt atctgcaagc catccgcccg gaagggttag caagtaaacg
    21841 gaagcagtat ataatccata aatgggcccg gaagtttcat tattgaggcc aagcccttgc
    21901 tgggcagccg gagtagtcat aaacaaaatg aggatggccc tcattccata atagctgaat
    21961 cgctcccaca tttcggtaaa aaaaagcgtg gctaatcccc tggggtgtcc aaaaaaagtt
    22021 tttccttctg tcataaagtg tttggttaat taagtgctaa agcctcttgc tccagcctga
    22081 aattcgcagg caccaggtat ttaaaaccct taaatctagt attgtatggg attaattaca
    22141 aatcagccat ttgagattcg ttttgctttt gttcgaaata accaaaagac aaaaagatat
    22201 aaagtgaatt agtcattttc gagtaatttt ggggcattca atcgatttcg gagttgaaag
    22261 aaaaagctaa aaatgaaaga atttggattt aagtctgaaa aatgcggatt aaatgaaatc
    22321 ggcatagaca accctgattc cgtttattgg aatctgtcgc ctgcagaact tgttgaagag
    22381 ggtttgagga ataatgaagg ggttttgact aatacgggag cctttatgtg cgacaccgga
    22441 aaatttaccg gaagggctcc aaaggacaga tatattgttg aggatgagac aacttctgaa
    22501 acgatctggt ggggagagat taatatccca atcacccaga aaaagtttga caatcttttc
    22561 aataaaattg taaagcatct tgatggtaaa aaactttatg tcaaagatgc ttacgcgggt
    22621 tcagacaaaa aatacagact taacctcaga gtaatcaaca cgtttgcctg gcacaacctt
    22681 ttctgtcaca atatgttcct gagaccaacc caaccagaat taaagtcttt tatacccact
    22741 tttactgtta taaatgcacc tgatttcctt gctgatccca aaatggatgg gataaaaaat
    22801 ccaaactttg taattattaa catgacaaag aaagtgataa tcgcaggtgg taccggctat
    22861 gcgggggaaa tgaagaaagg aatcttttct gttcttaact atttgctccc gcaaaaccac
    22921 caggtattgt caatgcattg ctcagcaaat attggagagg aaggagatac cgcaattttc
    22981 tttgggcttt ccggaacggg taaaactacg ttatcagccg atccgcaacg gggattaatt
    23041 ggtgatgacg aacatggatg gacagagaat ggcgttttta attttgaagg tggctgttat
    23101 gcaaaagcaa ttgatttgac tcgtgaaaaa gaacctcaaa ttttcgatgc tatccggttt
    23161 ggggctatcc tggagaatac caggttcaaa gaaggaagca gggatgtgga ttatacaaat
    23221 gtatctgtta ctgaaaatac ccggactgca tatccaattg atcatattga taatgcagta
    23281 atcccatcaa tcggcggtat cccaaaaaac attttctttc ttacatgcga tgcgtacggc
    23341 gtgcttccac ctatttcaaa actaaacaag ggacaggcga tgtatcactt tatttccgga
    23401 tatacagcca aagtagcagg aaccgaagct ggaataaccg atcctcagac tgtcttctcc
    23461 gcatgctttg gtgctccttt tcttccttta catccaacaa agtatgcaga gatgcttgga
    23521 aaaaaaatga ctgataacga tgtcaacatt tggcttgtaa atacaggttg gtctggcggg
    23581 ccctacggta ttggtgaacg catgaagttg aaatatacaa gaacaatgat atgctctgct
    23641 ttggaaggat ccctgcaatc aagtgagttt aagaaacatg atatattcgg tattgaattc
    23701 ccaacgtcat gtccaaatgt tccgccggaa atcctggatc cgaagaatac ctggaaagat
    23761 aaagaaggct atgacaataa agccagttac cttgcaaaag cctttattaa gaacttttca
    23821 aaatatgaaa attttgccaa tgaggaagtc ctgcaaggtg cgcctaagcc caatgttgca
    23881 gtttagataa gatatttcat aagaagagtt taagttgtca aacccgttat tttgttttga
    23941 aattgaaata aattttgttt gcatcagagg ttgatattca tcctgatact tggacagatt
    24001 ccaaaaattt ttcccatatt aacaacctgc agtttgaaga ccgattttgc atttaaacag
    24061 gaaaaggaga atttccggat tattacctgg tcatttgcaa agagttttct actctgtttg
    24121 tgaattctct tgattcatga aggttttgtt cgttaaattg agtggttaat tctctctgat
    24181 gaaaaaaact ttccgctcaa gacttcctta cctgatcctg ctgctaattc tatccctttc
    24241 acaatcggtc caatcggaag aatttaatct ttctgaaaca accaatatgg aggtgaccca
    24301 aaactctcaa ccagagtctg aaatagtgcc tgttggggat aaaaccgatc aaagtttagg
    24361 agtctcttta cttagaggac tgctaggagt gtcatcgata atcttgattg catttctgtt
    24421 tagctcaaac cggaaacaaa ttaactggaa ggttgtactt tcaggtctgg gaattcaatt
    24481 gatacttgct tttggaatcc tgaatattaa ttttattcaa aaattatttg aatttgtaag
    24541 ttccattttc atagccattc ttgatttcac caaggcaggt agtactttcc ttttcgggga
    24601 tttattaaat acagacagca ttggttttgt gtttgcattg caaatacttc ccacaattat
    24661 attcttctct gccttaacaa gcgttctatt ctatcttggg gtaattcaaa aaatagtgta
    24721 cggcctggcc tggttaatga ccaaggcctt gagattatcc ggagcggaga gcctgagtgt
    24781 tgccggaaat atttttcttg gacaaacgga atcacccctc atgataaagg cctacctgga
    24841 aaaaatgaat cgatcagaaa tattacttgt aatgattggc gggatggcaa cggtggccgg
    24901 gggtgtgttg gcagcctatg tcggtttttt gggaggcgat gatcctgcac aacaattact
    24961 atttgcaaaa cacctcctgg ctgcctctgt aatggccgct ccgggtgctg tcatcatttc
    25021 aaaaatgctt gtacctcaaa ctcaggcgat agattcaaat attcaaatca gctatgacag
    25081 tgcaggaagt aacatgctgg acgcaatggc aaacggcacc accgaaggat tgaaactggc
    25141 tttaaacatt ggggctatgc tcctggtttt cttcgctttt atcgcgttgt ttaatcatgt
    25201 cctgggttgg gttggaacta ccaccggact tgataccgcg attgcaggct tttcttccgg
    25261 gaggtatgat aatttgtctt tggaatttat cctcggctat gcattcgcac cattaatgtg
    25321 gctattgggc gtcgcatcgc atgatataac cttagtagga caattactgg gtcaaaaaat
    25381 tattgcaagt gaatttgtgg gatatgaaag cctggcaaca ctcaaaaatt ccggagtctt
    25441 aactgacaca aaatccataa taatgtcgac ctacatgctt tgcggttttg caaacttcgc
    25501 ctcaattggt attcagattg ggggtatcgg cgggttggct cccgggcaaa gagttttact
    25561 atcagaattt ggatttaaag cattgctagg aggaacgtta gcgtcactac tgtcagcaac
    25621 aattgtcgga atgattttag gctaattacg cttgttaatt tcgtctctga tttttgcggc
    25681 acgctcgtag tcttcatcat caatggcttc tttcagaatt tcattcaact tatcaataga
    25741 aaggtctttt aatgaagcaa tggtttcatg ttttttgggc ttaacaattt cagttttgag
    25801 acttgccact ccttcagctt cttccgtgag tacgatgccg gcttcggcca ggatggtttc
    25861 gtatgtgaaa atgggtgcat cgaaccttat tccgattgcg attgcatccg agggacgtgc
    25921 atcaatttct attgttcctt ttccgttgga acaaactatt ttggcaaaga atacgccttc
    25981 tttcaggtct gatataagaa tttcttcaac ctgatattta tagctggctg cgaatgactt
    26041 gaaaagatca tgcgtcattg gtctgttggg aataatcttt tctatctcaa tagcgattgc
    26101 ctgggcctca aacattccaa taatgattgg cagcctgcgg tttccttttt cctcccccaa
    26161 aaccaaagca aatgatcctg tttgtgattg gctggatgaa agtcccagaa tctctaactt
    26221 aattttatcc acactcagtc tatttacttg ctctaatagc ctccgttaat ttaggaacaa
    26281 cttcaaatgc atcgccggca atcccataat ccgcagcttt aaaaaatggc gcttcgggat
    26341 ccttattaat cactacaatc acttttgatg aattaactcc agctaaatgc tgaatggcac
    26401 cggaaatacc aatagcaata tacaaattag ggccaacttt cagcccggtt tggcccacat
    26461 gttcatgatg aggccgccat tccatatctg aaacaggctt gctgcacgcg gtggccgcac
    26521 ctaattcttt ggccaattct tctaacatcc cccagttttc aggacctttc attcctcgcc
    26581 cgccagatac cacaatctca gcttcgggca acaacacatc tcccgaagct tgatcggttc
    26641 cggttatttt caatgcgaaa tccgccggat ttaaatctac agaaaaagtt tcaaccggag
    26701 ctttgcttcc tgattctgtt aactggaccg cgttttttct gattgcaagg attttatatt
    26761 cgttattcaa ttcagtaacg gcaaaggctt ttcccgtata aatactccgt ttaaccctga
    26821 agcctgttga cgtatcgggc aattcagtta cattggaagc cagcgatgca tttaacttca
    26881 cagcagtacg ggctgctacc ggggcgccca gggatgaatt cgcaagaaca agaatttttg
    26941 aatccgtttt tctcatcgct tcactaatca ccgtagcgta tgcgggaata actccctgat
    27001 tcaacttatc gttattcaca tgcagaactt tgccggcacc aaacttcccg agggcttcca
    27061 gctcttcagt tgtgatatca cttcccaaat aaattgcaac agcattctta tcaatggccg
    27121 cggcaaaaga cacagcttct tgcgaagttt tcttaacttt tttatccgaa ctttcaataa
    27181 atacgagtac agacatttct ttctttataa gactttagct tcatttttca acaacttaac
    27241 cagctctgaa acattttcag gatctatcat tttaacttgc ccttttgctg gcggaagttc
    27301 gtaactaacc aattttgtcg attgaacatt tccttttggt tccactacat ttaggggttt
    27361 tgttctggcg gacataattc ctctcatatt tggaatcttc cattcagcga ttggctcctg
    27421 gcaacccgcg acaaatggta gctcaacttc tacgtattcc ttgccccctt caatttcccg
    27481 ggccattttt gcttttgttc catctagttc caaagacata accggtgaaa ttgacggaat
    27541 gcccagcaac tcccctacca tcccatgtac cattccacca ttaaaatcga ttgattcccg
    27601 gcccatcaaa atcatgtcat aattatgttc tttggcaaca tttgcaattt catttgacac
    27661 aaaaaaacta tcggcaggat tggcatttac ccggatggca tcatcagcac caatcgcaag
    27721 tgcttttctt attgtgggat cagattcagg tccgccaaca ttaagtacgg taagcgaggc
    27781 tcctgttgct tccttgatct caacagccct tgcaagggca tagtcatcgt atggaccaat
    27841 aataaactga actccattgg tattaaactt tgtattattt tctgtgaaag aaattctgga
    27901 ggtggtatcg ggaacatgag taatacaaac cagtattttc attgattagg gcttatttct
    27961 aaggtttttt agtgcttata ggtcgcgaat ttaagtatta tggcccataa ataaaatgaa
    28021 ataggtgtat tcaacagtgt attgaaaagc aagcccgttt gtgataatgt tagtaaggaa
    28081 ttagaaatac cttcgctcaa atctctgccg gctgaagggg caaaatccag ggcaagcaat
    28141 aattcgtgta taccatagtg ggaggagatt aaggaatttg cgggtagttc aaagaaatat
    28201 cctaaccgga attccttttt aacagactat taatttcatc ttgttttggg ggaacgtatt
    28261 caatattaac aattttgaga ggtcatgcaa gtcaacttca ttttgaaacc cactatttaa
    28321 acaatgaagc tatttttata cttttgaaac catggaagta atcaacaaaa aatcaataat
    28381 ctcaattttt gtcctgatta ttcttgttct cattttgatt gcagtattct ataataacaa
    28441 ccctcaccag gaaaaacaaa ttaatttgca aggtattgcc tcacgttatc tgaaggctta
    28501 tccgctccct gccggctgga ataatgatcc aaatctctcc gataaatatg cagcattcag
    28561 attatatagt cagcgtaatt atttagaagc aatggtaaat ttaaagtata ctctcgtcaa
    28621 taatcctggg gataaggaag tgaatttcta tttgggtatc tgcatgctgc tgacaaacaa
    28681 acctcgtgat tccatccggg aattcaatat aattattgag tctgaaaatg atccgctaat
    28741 aaatgatgcg aagtggtaca aagcattggc cttgattaaa cttgacagaa ttcctcaggc
    28801 aaaacgattg cttggtgaat tgagttcaga agattcaata aaactattgg atgaaatctc
    28861 ggtggggaat taaaatggtg ccggtccggg gaggtcatct tcatccctgc ctgcattcat
    28921 tcgacttcca aatgtcacgg tgctgccgct gcttccactt cctgagaatg atgaatgcgt
    28981 atccggttct gtgaatttgg tatatttggc aatgaattta agtggtatgc tatccaggct
    29041 tccgtttctg tgcttggcaa taatcaattc ggccatggaa gcagtcggga ttccgttttc
    29101 atcttcagta attccatagt attcaggtct gtaaaggaag ataaccaaat ccgcatcctg
    29161 ctcaatagat ccggattccc tgaggtcgct aagctgaggt cttttgtcac cgccgcgggt
    29221 ttccaccgca cgactcaact gcgaaattgc aacaactggt atttctaatt ccttggccag
    29281 gtttttcagg gcccggctta tagatgcaat ttcctgttcc cggtttccac ctgaattttt
    29341 agacatatcc ccgctcatta actggaggta atcaactata actaattgta tatcatgttg
    29401 agctttaagt cttctgcttt tagctcttag ttcaaagatg gttaaagccg gagtgtcatc
    29461 aatgaaaata ggcgcactgg taagttttgc cgttttgtga actaattgct cccactcaaa
    29521 ttccaccagg tttccttttt taattttttc gctttccaat tctgcttcag ctgaaatcag
    29581 acgattaact aattgaaccg atgacatttc cagagagaat atcgccacag gcttttcaaa
    29641 atccaccgcg gcatttcgca tagcagaaac gacgagcgcg gtttttccca tacctggccg
    29701 tgcagccaga ataattagat ctgatttctg ccaaccgttg gtgattcgat ccagattagt
    29761 aaatcctgaa ggcacaccgg ttagtccatc cttgtggtgc ttccgggctt caagttcatc
    29821 catagcttcc ttcatgatag accgcatgtc ggcatagttt ctccggatat tggtttcaga
    29881 aacatcaaaa agagattgtt ccgtccgatc cagcaaatca aacacatcaa tggtatcctc
    29941 ataagcgtct ctatgcactt cggatgcgat gcggatcaat tcgcgcttaa tggcctgctg
    30001 agtaataatc ctggcatgag tttcgatgtt agcggcagaa tttactttgc tggttaattg
    30061 cgtgatgtag taagcgccac cggcaaattc aagcttacca tcacttttta actgattagt
    30121 aacggttaga atgtcaaccg gctcagaatt attgaacagt atctggattg acttatatat
    30181 ctcctggtgc gaatctttat aaaagctttc aggtttcagg atatcaataa ctgttgaaag
    30241 cgcatctttc tctaacatta aagcacctaa aaccgcttcc tcaatttcaa gggcttgtgg
    30301 tggaattttt ccaattcctt cattaatatc acttgcggaa aagaccgggt ttttgtatcc
    30361 cagccggatt gaacttgtct ttcgctccat ttcgtctcta agggttgatt atttgggttc
    30421 gttttggagc tcaaacttgg ttaaaaaagt taaggattgc gataagcagt ctattcatct
    30481 tcttaacatt ttatccagca caccaaaact tatccacatc tcaatttacg aaaattggtc
    30541 gatttgggcc ttatccacaa tcaattcttt tgtgaaacca actatccaca tgaataagca
    30601 ttttatcctt aacttatcca cagaatcttt agctgaatcc acattcaaac cgggttttcc
    30661 acctattgtt attttgactt atttttgcag cattaaaaat tagatgaaca aagaattgaa
    30721 tagaattcac tttatcgcga tgggaggaag tgttatgcat caacttgcca ttgccctggc
    30781 cagggaaggg agaaatatca ccggttctga tgatgaattc tttgagcctt caaaaagcct
    30841 gttggaaaaa tcagggttac tacctgtgaa agccgggtgg ttcgctgaaa aaataaccga
    30901 caaactggat gcagttatac tggggatgca cgcccgcagg gataatcctg aattgcagaa
    30961 ggcatttgaa cttactatcc ctatttattc attccctgaa tttatctatc tccactccaa
    31021 agacaagcag cgatttgtca ttagcggaag tcatggtaaa actactatca ctgccatgat
    31081 cattcacgtt ttaaattatt ataagaagga atttgattat gttatagggg caaaggtcga
    31141 tgggcttgat tcttgggtaa aactttctga tgcacctgta attattctgg aaggagacga
    31201 atatgcttct tcacccctgg ataagactcc aaaatttatg aagtataaac accatatcgg
    31261 agttattaat gggattgcct gggatcatat gaatatcttt cacaccgagg aagcctactt
    31321 acgtgctttt gaagattttg ctgatgccac tccaaaggca ggatcactat tttattgtga
    31381 agaagacagt cttacatcaa tgattgggat taaacagcgg gtggatgtaa attcaattga
    31441 atataaatca tttccattcg aaacaaaaaa tggaaatatt tttattaaag acgagactga
    31501 acttttcccg gtaaaggttt tcggtaagca taatatgcaa aatatgggtg cagcccaggc
    31561 tgttttacgc cggcttggta tttcaagcaa agaatttctt caggcaatgg gtagttttac
    31621 gggagcaagc agccgtctgg aagtgctggc tcagacaaaa tcagcaacag tatataagga
    31681 ttatgcccac agtccttcaa aagtattggc caccatggat gccgtcaggg agcaacatcc
    31741 tggtattaag tttatcgctt gttttgaatt acatacctat tccagtctga attctgaatt
    31801 tataaagaat tataaagatt ccctgaataa agctgaccaa gccattgtct attataatca
    31861 ggaaacttcc ttgcataaga aattaaaccc gctgacatca gaagaaataa agaactcatt
    31921 caataatgaa aagttggcaa ttgtcaataa ttcaagcgac cttaaagctc agcttgattc
    31981 tcttagcggt acttttgtgc ttttgctgat gagttcagga aattttggtg agctcaatct
    32041 taaagaaatc gctcaaaaaa ttacttctta atgattatta atctcagaaa tcccattgcc
    32101 ttctttgatt tagagactac cggggtcaat atctcccagg atcgaattat ttctatctcg
    32161 ttaattaaaa tttttccagg gaatgaacag gaaaagaagg aatatttaat caatcctgga
    32221 attccaatcc ctgctgaatc cagtcttatt catggaatat atgatgagga tattaaaggt
    32281 aaaccctcat tcaaggagat cgcccgaaac ctggccaaat ttcttgaggg gacagatctg
    32341 gcaggattca gcataattaa ttttgatgta cccatgctcg ttgaggagtt tctccgggcg
    32401 gaagtagaat tcgatatcag gaagaaaaaa atagtcgatg cacagaaaat ttttcacctg
    32461 atggaaaaga gaaccttaac tgccgcttat aaattctatt gcaacaaaga gttggctaac
    32521 gcccataatg catcagcaga tactctggcc agtctggaag ttctggaagc ccaggtacaa
    32581 aagtattctg gtaatgaagt gacggacttg ctcgggaatc ctcttggcca attggagaat
    32641 gacatggaag tactccataa gcttaccatt tcaaataaag tagaccttgc cgggagattg
    32701 gcatttgatg ataacggcat tgaagttttt aattttggaa aacacaaggg caaaaaagta
    32761 actgacattt ttgaacagga accccagtac tacgattgga ttatgaaagg agattttcct
    32821 cttgatacaa agcggagaat cacagaaata aaacttcgtg gatttaagaa gtaggtctaa
    32881 ttctttttgt aatatcccat agcttcaggc atccaggcat tcagatccga aatccttctt
    32941 tcatgagatg ggtgagtgga aagaaattcc ggcggctgtc cccctgttcc ggaagtcatt
    33001 ctttgccaaa aagcgggtgc ggcctgtggg tcatagccgg caattgccat aaatatgagc
    33061 ccgatatggt cagcctccga ctcgtggttc ctggaaaatt tcaacattcc catttgcgtt
    33121 ccaattccaa tagattgcag aaatatatca ttggttagcg atggattttg tcccattgcc
    33181 attgacaagg ttgacaaacc caggttcgcc agcaagccct gactcatcct ttcacgaccg
    33241 tgattagcaa ttgcatgcgc aacctcatgt cccattacca cggcaactcc cagctcatcc
    33301 tgacatacgg gcaggatacc cgtataaaat gcaaccttcc ctcccggcat agcccaggca
    33361 tttatagtgg tgtcatcttc aataaggttg aactcccata gaaaaccctg aagctgattt
    33421 gcagatcctt gttcggttaa atatacttcc accgcatctt tgatccgcgt ccctactctc
    33481 ttaatcatat ccacctgttg ctggttttgc gacaatttac tttctgcaat aacctctttg
    33541 tagctttcga aactcatgat catgagctcc tgggatgaaa tgaggctaag ctgacttcgg
    33601 ccggttaccg gcacttttgc acaactaaat actcctaaaa ttatcagcgc cccagaaaat
    33661 aatctgctta tcattcttgt tgatttgatt tcaaaaataa taatccttgc tgattaaaag
    33721 tttttaaatg atataaatct tgaggtttta ctttattatc cacatatccc cttaattttg
    33781 tgaactaaac cttgtttatg aaaatttttg gaattggcag gaattatgcc gagcatattc
    33841 aggaactaaa aaatgaaact cccgatgatc cggttatttt cttcaaaccg gatacggcct
    33901 tgctgcgtaa caatgatcct ttttactatc ctgacttcag caaggatatt cattacgaag
    33961 tagagattgt tttacgaatt tgtaagaacg gaaaaaatat tgaatcaaaa tttgcaagaa
    34021 cttattttga ccaaataggg atcggtatag attttactgc aagggattta caggaaaagg
    34081 caaaggaaaa aggattaccc tgggctatag ccaagggatt caaccattca gcgcctattt
    34141 ctgagtttta ccctgttggt gattttaaag atttggataa tctggaattt ggactggaaa
    34201 ttgatggagt acttaaacag aagggtaata ccagcatgat gctattcggt tatgataaga
    34261 tcattactta catttcaaaa ttctttatgt tgaaaatcgg cgatcttatc tttaccggaa
    34321 ctccaaaagg cgttggaccg gtaaaaattg gaaatcgatt aagagcattc cttgaagata
    34381 aaacattgtt ggattttgaa gttaagtaag tgtctctctt tcctggttct tatttatttt
    34441 ccgatcctcg cgcaaaatgg caacggggaa tcggattaca gaagatatat tctcccggta
    34501 ttccccaatc aaaccaatac gcttgccgga acaatgggag agttgcgtaa tacgcacttc
    34561 catacaggaa tcgacattcg cacacaaggc aaaaccggct tacctattgt tgccgcggct
    34621 gatggataca tcaacaggat ttcagtgcaa ggatcgggat atggaaatgc tttatatatt
    34681 caacatgctg acggcaatat tacggtgtac gcccacctta ataatttcaa tgatgaaata
    34741 gcagaatttg tgaggcaaga gcaatataaa cgacagaagt ttgaaatcaa tctattcccg
    34801 gctcaaaatc aatttcctgt gaggcaggga gatcttattg cattaagcgg caattcggga
    34861 tcatcggtgg ggccgcacct gcattttgat cttcgcgatg aaaatcaaaa tctgctcaac
    34921 ccacttgagt acggttttca tgaggttatt gatgatatac ctcctgtagc cagaaagatt
    34981 gccgtctcaa ctctggatat caattcaaga atagccgggg aatttggacg gatggaattc
    35041 gatctgattc gacaagggga taaatatacg gtagcggata ccttgctggc ttcaggatta
    35101 attggacttg aaatttacgc ctatgatcgg caaaactata ccttcttcag aaccgggatc
    35161 cccacattca aggtttttat taataaacag caggtcttta accaaaatat catcgctttt
    35221 tccttttccg atcagaagca tattaacgtt cacaccaatt accaaaaaaa agtagacacc
    35281 ggtagaagtt ttgccaagct ttatattgac gatggcaatg aactaccatt tttccaggct
    35341 ttcaataaag gaaagattaa ggtaactgag ggagttgtgc ttccagttac ttttgaatta
    35401 aaagacacgt ataataatag taccattgta gaatttttta ttaaaggtca aaaatctgca
    35461 ggttcatata agtctacctc caaaatctct tcaggaattc ggtccgaaat aaaagagaac
    35521 actttgatta tcaggtgtaa taaagattcg ttattcaata atcaattgaa atactttagc
    35581 aaagggatgg agtcccttgc ttacccggcc tatatcacag gaaatggttc ggcagtcttt
    35641 cttttcgacc tgcgaaaggt tttggtggac tccgtttcga tttctaattc aaaaaaatat
    35701 tataatttta aagccatggt tccctctggc attccatata gcttttatag caactctatt
    35761 gatatcaact ttagtcgaaa ttcccttttt gatacagttt ttctagctac ccggtatttc
    35821 gttaatgata ataatcagga aattttccac ataaacgaaa atacagagcc gctaaaaaat
    35881 tcaattaaag taacacttaa aacaaggaac tcatatcctt ctaaaactga ggtttataca
    35941 attgattcta aagggaatct cggctatatg ggcggatcct gggacgggaa atcaattatc
    36001 ttcagtacaa caacttttgg agattatact ctcgcctccg attccatccc acctgtaata
    36061 aggcccgtca tcgttaattc caataattta acattcatta tagaagataa aatgtccggg
    36121 ataaaagagt ttagggctat ggtaaatgga aaatggctat taatgaatta tgatcccaaa
    36181 aaaaatctta tctggtcaga gaaacttaaa cccgagacca ggttagccgg tgatgtaatt
    36241 ctaaaggtta ccgataaggc tggcaatgaa agtatatacc aaactaaact ttagacctga
    36301 tgacattaaa tgttggggac aaagcccctg atttcaaaat aaaaaaccag gacggaaaaa
    36361 ccgtaagttt aaaagattat aaaggaaaga aaattgtatt atacttttat ccaaaggata
    36421 atactcccgg ctgcacctcg gaatcctgta acctgcgaga caattacagt gcgcttagaa
    36481 aaaagggata tgaagtgatc ggaataagct cggattctga aaaatctcac cgaaatttta
    36541 ttaaaaagtt ccaattaccg tttactctcc ttgctgatat tgaaaaagaa gttcatcacc
    36601 attatggaac ctgggtggaa aagtcgatgt acggaaggaa atacatggga accgccaggg
    36661 ttacattcat catcaatgaa aaaggaatta ttgaaaaaat tattgacaag gtcaaaaccg
    36721 gcgaccacac caatcaaata ttgacttaat gattgatcaa aaagaaatcc aaaaactaaa
    36781 agacctgacc ttccaggtaa gaagagatat cgtcagaatg gtgcatgctg ttcagtctgg
    36841 ccatcccggt ggttcgttgg gctgcacgga attttttgta gcaatgtact ttcatcacct
    36901 tcggcataat aatgattttt caatggaagg aaaaaatgaa gacatgtttt ttctttctaa
    36961 tggtcatatc tccccggtgt ggtacagtgt cttggcaaga agcggatact ttccgttatc
    37021 agaattgaag actttcagaa aaattgatag tcgtctgcag ggacatcctg caacccatga
    37081 aggtttaccc ggggttcgca tcgcctcagg ttctctcggc cagggattat cagcagcgat
    37141 tggggccgcc ctggcaaaga aattgaacgg tgatccacat tttgtatttg tattgatggg
    37201 tgacggtgag caacaggaag gacaagtttg ggaggcaagt atgtttgcag ctcattataa
    37261 gatcgataat ctgattgctt cgattgatgt aaatggtcag cagatcgatg gtcctgttaa
    37321 tgaagttatc tcacttaaaa accttaaggc taaatgggaa gcttttggat ggaatgtaat
    37381 tgagagtaat ggcaacgatc tggaaaacat tatagataaa atagaggaag caaagtcgct
    37441 caccggtaaa ggccaaccgg tattgaatct gatgactaca gtaatgggaa agggagtcga
    37501 tttcatggaa ggaagtcata agtggcacgg cattgccccc aatgatgaac aactggaaag
    37561 cgcgcttgcc caacttgaag aaactattgg agactattaa taccaactac cgtgaaccaa
    37621 accgtattga aaactaaatt tactttcacc gaaaaaaagg atacccggtc aggttttgga
    37681 gcaggattac tggaggtagg cagaggtaat gaccagatcg tagccctgtg cgctgacctc
    37741 accggctcct taaaaatgga tgcgtttcag aaggaatttc ctgagagatt tttccaaacc
    37801 ggtgttgctg aagctaatat gatgggtatt gcggccggct tagccacttc cggaaaaatt
    37861 ccttttacag gtacctttgc caacttttca acaggcaggg tttatgatac tatccggcaa
    37921 tccattgcct attctggaaa aaatgtaaag atttgcgcct ctcacgcggg acttaccctg
    37981 ggtgaagatg gagccactca tcagattctt gaagatatag gaatgatgaa aatgcttccg
    38041 ggcatgacgg tgattaatcc atgtgactat aaccaaacca aagcagctac acttgcaatt
    38101 tcagaacatt atggtccggt ttacctgcgt tttggcagac caggatggcc aattttcatc
    38161 ccggctgaga caaagtttga aattggtaaa gcactgccac tgatagaagg atcagacgtg
    38221 agcatctttg caaccggcca catggtgtgg caagccattg aagctgaagc tattctggca
    38281 gaaaaaggcg taagtgctga agtgattaat atacatacca taaaaccact ggataaagaa
    38341 gcaatccttg attctgttgc taaaaccggt tgcgtcgtaa ctgctgaaga acatcaaatg
    38401 aatggaggtt tgggagaaag tattgccagg ctcctggcgc agaaaaggcc aacaccactt
    38461 gaattggttg ctgtccagga tactttcggt gaaagtggta aacctctgga tcttttgaaa
    38521 aaatacggat tatcacctga gaatattgtc ggagcggcaa ttaaagtgat aaatagaaaa
    38581 taaacggatt gccctgccat ttgggccagg ccgaaaaaac caaattttgg aacgttaatc
    38641 cggcagcaat gaccacatct cggaaaactt tctttgaatt gcaggcacaa acttcacctc
    38701 atccttttgg actggagatt gaacgcgccg aaggattata tatattcgac agacagggac
    38761 gaaaatatat ggatatggtt gcaggacttg ctgttactaa tataggccac cgacatccaa
    38821 agattgttga agccataaaa aagcaggcag atactcacct gcacattact ccatacggag
    38881 aatttattca acttccacag attatacttg ccgaaaaact tgcttccatt cttcccgcga
    38941 atttgaattg ttcttacttt gtgaactctg gcactgaagc taatgaagga gcggttaaac
    39001 tcgctaaacg cgctaccggc agaagaaaag ttattgcctt ccacaaatct tatcatggaa
    39061 gcacgcatgg atcgctttct ttttcaggaa atgaaaaaaa gaaatccgca ttccggccgc
    39121 tgcttccgga agtttacttt ctcaaattca attccgaaga ggatctggag ctaatagata
    39181 ataaaacagc ctgtgtcatt atggaaactg ttcagggaga tgcgggagta agaataccca
    39241 gtcattcttt tatgaagaaa ttgcgtgata aatgttccaa aaccggagct ttgttaattt
    39301 tggatgaaat tcaaaccgga ttcggaagaa ccggtaaaat gttcgccttc gagcattata
    39361 gcatcgtacc tgatatttta accctggcta aagcccttgg tggtgggatg cccattggag
    39421 cgtttatctc atcgaaagaa attatggcta agctttccta tgaccccatg ctgggacata
    39481 taacaacttt tggtggacac ccggttagct gcgccgccgc cgccgcaaac attggtgtat
    39541 tacttgatga aaaattaatt gatcctgttg aacaaaaagg cgcactattg caggaacttc
    39601 tggaccatcc gaaaattaat gaaattcgaa ggataggctt aatgcttgcc ctggagtttg
    39661 attcccgtga aacggtggaa aaaattgtaa aaggttgtat ggaaaatgga gttatttgtt
    39721 tttggtttct tagttgtccg gagagtttca ggttatcgcc gccattaact atttcagaag
    39781 aagaaatgga aatagctgcc ctggtaatta agcaggaaat tgaaagtcac acctaggtta
    39841 atttgcaccg ggacagtacg ggtagagcaa tcgccaaaat tatcagaaga acattttgcg
    39901 atcttccacg ttgtgattat ctggttcttt ggatggcttt tttatcgaaa aaaaaataat
    39961 aaagtagaat tacctgccgg gagcgcttgt atccctgcca tatatttctt ccagctttgt
    40021 atgcaaatct ttgccacgaa ggttttttgc aatgattttt ccctccggat ccagaagaac
    40081 tgaaaatgga attgcagaaa cgttgtaggc ccgggccgct tctgaattaa agtaattcaa
    40141 atcagaaaca tggttccagg gcaatccatc ctcggcaata cctttgagcc atttatctct
    40201 gctcctgtcc agggatacac ccagaatttt aaatccctga tctttgaaac gattataagc
    40261 tctcagcaca tttggattct ccatcctgca cggcttacac cattcggccc aaaaatctat
    40321 cagcacataa tcacctctca aagaacttaa tttgaagatg ccaccctcag gtgttgggag
    40381 agagatttcc ggcgccatct gtccaatagc cacagctctt aacctttcca cttcattagc
    40441 caggttctgt acgaaaggta cgttaggata tttctgaatg agtttgctca ccacattgtc
    40501 gaaaaattca aattcttcat ccttattaat cagacttaaa gcctgtaccg tagctaaaga
    40561 aattcccatt cgctctattt cctgtttgat catttcagtt ttttcaccct gaagaagcat
    40621 gtactccccg gtgagttttt gggctttttc agagtcatta accgcaacag cctgtgcata
    40681 ctcctgattc aactcagatt caagctggcg atattcttcc accaaagttg ctatctcgcg
    40741 gagcttttca acatcagaag atccggttat ctgactaatt ccattctgac ttcccccatc
    40801 cgcagcaacg tggatatcat cttcatccag aattacagta ataaactgtt tattgaagaa
    40861 attcaaccgg aaaaaacctg gatttgaaag ataaatttcc ctggaaaatg tattattctg
    40921 gttcagcgga attgtatcgt atacgaggta tgaattactc tgaagaacaa attcctccag
    40981 cagaattaat ccgggctgaa tattttgaac ttcaccagta atagtaacag accttgtagg
    41041 tagcgaaacc aggtcagtct tcttgtcaca ggatattaga ataaaagtcg caccggtagt
    41101 aattaaaacg gataaaacaa gtgtcttcat aatctgacaa agttaaagta cacaatagaa
    41161 aaagaagaaa ttaagttcac caaatgggaa aatgaaccaa aatttttatt gatctaatgg
    41221 atgaattacc tttgtgatgg caaacgggta cgactagctc ctgtcgaact cccccaggtc
    41281 ctgacggaag caagggtaga tggttgtagc ggtgtgatgc ttcgtttgcc tttttcttta
    41341 agtggaaatt aattaactac cccgattcac cctccttttt gattttttta tagctttgta
    41401 cattatctct aaattagact tatatgaagt tttttattga cactgcaaac ctttcagaaa
    41461 ttcaggaagc ctttgatttg ggcgttttgg atggagtaac cacgaatcca tcattaatgg
    41521 ccaaagaagg tgtccggggt aaggataata tcaaggcaca ttacaaagcg atttgcaata
    41581 ttgttgataa taatgtttcc gcggaggtaa tagctacaga ctttgatggc attataaggg
    41641 aggcacacga attggcgaag atagataaca aaatcgtggt gaaagtcccc atgataaagg
    41701 aaggtgtgaa agccatcaag tatctttcga cagaaggaat tagaaccaat tgtacgctca
    41761 tattttctgc aggacaggca ttgctggcag cgaaagccgg ggcatcttac gtttctcctt
    41821 ttattggcag gttggatgat gtctcctctg atggtgttgc cctgattggg cagattgtct
    41881 tgatttattc caattacgga tataagactg aggttctggc agcctccatt cgtcacacta
    41941 tgcacctgat tcaatgtgct gaaattggcg cggatgtggc cacttgcccg ctcaaagtaa
    42001 ttaccggtct tttacaacac ccacttaccg acaaagggtt ggcgcagttt cttgcagatc
    42061 ataagaaggc aaacgagaag gtatcagtat agtgatgagc ttttccagcg atcccgtagt
    42121 ccgggtacaa aatgctacaa tttttcagga cgaaaaagct gttcttgaag atatcaactt
    42181 tgaggttgca aaaggagaat ttgtgtttct ggttggaaga accggaagtg gtaagtcatc
    42241 gctcctaaaa actctgtacg ccgatcttcc tcttcgactg ggtgatattg aagttacagg
    42301 atatgatatc aggaaaatca agagtaagga tattccatat ttacgcagaa agattggaat
    42361 tgtattccag gatttccatt tattcccgga ccgatctgtt gctgagaacc tttattttgt
    42421 aatgaaggcc accggatgga gagacaattc caaaatgaaa tccagggcaa ccgatgtttt
    42481 gatgcaagtt gggttaggag ccgtttcggg caaaatgcct catcaactgt caggaggtga
    42541 acaacaacgc gtagtaattg ccagggccct cattaatgaa cctgcaattt tcatcgccga
    42601 tgaaccaacg ggtaatcttg atcctgctgt ttccgaaggt atttttaaag tttttcagga
    42661 aataaataat agcggaactg ccgtaatgat ggctacccat gatcattact tcattaagaa
    42721 gtatccgaac agaattctga aatgttcgga aggcaaggtt caggattcaa gaaataaaga
    42781 gattaacctg gaagaaatgc tctagctctt tgttgcaggc cgttcttcta atgcttcatc
    42841 ttcctgcatt ttttccaacc gatccaattc cctgttaagt atatctaccc ttgaaatcag
    42901 attcaaaatc agggcatcaa tcaccggctg gctgggatct ttttctaatt cggttttcaa
    42961 tacctggtag atagcattaa gattccctaa ttgagccata taatcaaagt cgacattgtc
    43021 ggcgtaatta tccaattcct gttctttgag ggaaatgagg cttgtataat aattttccgt
    43081 ttcaataaac tgatcggtgt taccggtaag tttgccggcg cgttcagaat taattaaaaa
    43141 atcattcagt agtaaaaatg ctgataagaa aaagaaaaac acagctgccg ctttccacat
    43201 ccagaagccg gaagtactcg cgcgttttcc cgaaatttca cgctgaactc tcttccatac
    43261 tctatcgccg ggcgtttcca aatcaaaaga ttctctgttt tcagaaacaa atttttcaaa
    43321 tctatctttc ataggttctc tcctttccaa ttaaatctac aattttttgt tttgccctca
    43381 gatattgagt ttttgaggtt gattcagata ttcccataat ttgagcaatt tccttgtgat
    43441 catatccttc cagacaatac aaacttaata ccactctgta tccatcaggc aattcctgaa
    43501 tggctttttg tactgttttc actttgaatt ccatatccgg ctcattctcc actttgtctt
    43561 ccaattcata atcaactgaa agttccacta aattggcttt ttgttttttc aaatgattta
    43621 tggccttatt aataacaatg cgttttagcc aggctccgaa tgttgctttt ccgctaaatg
    43681 agtgaagatt ggtgaatgca ctgacaaagg catcctgaag gatgtcttca gcatcatcac
    43741 tatcccttgc aatacgcaag gcaacattga acatggcttt actgtataat ttatataatt
    43801 cactgtaagc agattcatct ccggctaaac aggcatccac cagatcctgg tggatattga
    43861 cataatcgtt acccatcggg gaatccactt atcatttgaa ttaatagact acactgatgt
    43921 tgagaagttg catagccaga taattattta agagatgcca aattttcttt agccaatttg
    43981 aagttgggag ccagcgccaa aacttcttca aaagtttttc ttgcttctac tgtatcgcct
    44041 tgttggatga ggagagctcc tttcaggttc aaaactgaca ccctcatggg aaattcctcc
    44101 tgcttatttt cctcaaaagt gtaaacaagc tttaattcat ctacttcctt ttttgataaa
    44161 agaatatcaa tattagtttc acattcatca tatcttttca attcgaactg aataaatgtg
    44221 gttttataaa gtgaattgat attatcagtt aataaataca gtttttcata agcctccagc
    44281 gacttttgca gtaagccaag attttcgtat gatacagcca tcgcttcaat aattcccagt
    44341 tcattaggat tctttgcaat gatatccata caaaccatta aacaagaagc atagctttgt
    44401 gaatcaaaat aataatacgc cagcgtataa agtaccgaat cattttcggg atcagctgcc
    44461 agcatattat ataatgcatt tttagccacc ggaaagtcgt tgtatttcat cgctctatca
    44521 tatacttttt tgaatctctc ataatcctgc tttcctgtct gtgaaaaacc gctcaaagaa
    44581 attaccatca ttactagtat tactactatt ctattcattt tattgttcaa ttatgtttcc
    44641 tgattttttg acttttaaac cattttgctt tcctatttta atcatatagg cgtaagcttc
    44701 ctcaaaatta ttcttaattt ttccttcaat tactgcctcc ttaatttctt cttttattgc
    44761 acctactact ttaccaggtt taatatcaaa gacttccatt atttgcaagc ctgttacagg
    44821 aggttggaaa ttgcgaattc tatccctttc ttccacttcc tgcatttttc gttccacatt
    44881 atcaaagtta gccagatacc gtttcacctt gatgtcattt tttgaggtga tatcagccct
    44941 gcaaagagac attaaggagt caatatcttt tccggcttca agcaaaagtc tgcgcaaagc
    45001 ggaatcagtt atatgatctt ttaccaatga aatcggcctg agatgcaact ttacaagttt
    45061 ttgcacaaat ttcatgttct cgttcagtgg tagtttcaac cttcggaata ttccaggcac
    45121 cattcgcgaa cccttatctt catgaccatg gaatgtccag cctgcttttt tgtcaaatct
    45181 ttttgttgcc ggcttggcaa tatcatgcaa gatagcggcc catcttagcc aaaggtcatc
    45241 agaatacttt gcaacattat caagcacctt aagggtgtga tagaaattat ctttatgagc
    45301 cagtccctca ataatttcta caccggccag ctcaacaatt tcaggaaaaa ttattttcag
    45361 aaggcctgat tcatacaaca ggtttaatcc ataggaagga ttttcactca gaataatttt
    45421 gtttaattca tctgtaattc tctcctggct aacaatattt attcgctccg cattatttat
    45481 tatggcttca aaagtatcgg gttcaatatc aaatttcaat tgcgctgaaa accggatcgc
    45541 ccgtatcatt ctcagaggat catcagaaaa agtaattttc ggatcaagcg gagtccgaat
    45601 gattttcttc tttaagtctt ttataccacc aaaaggatcc agaagagtac ctaaatcatt
    45661 ttcgtttaaa cttattgcca gggcattgat cgtaaaatca cgcctgtttt gatcatcctc
    45721 aagggtacca tcttccacaa ttggtttcct tgagcttgcc ctgtaggatt cctttcgggc
    45781 accaacaaat tgaagttcct gatcttcaat ttttagcatt gccgttccaa agtttttgaa
    45841 agtggccaat gaccccttac ccgatttagc tttcactttt ttcgccagat caattccgct
    45901 gccaatgcaa acaaaatcca gatctttgcc gtttttccca ttttttttca acaacaaatc
    45961 ccttacgtat cctccaacaa cgtacgtttc tataccttca tcggaagcaa cttccgccac
    46021 cagggaaagc attggactac cggaaatata agatttgaac tgcatttgaa tattcaaact
    46081 caaaattaag acaataaata aaaagaaaag ggtttagatt aacgtttcca gaaggatttc
    46141 gagttaaaag tgggcaagga ataaatttgt cctttcgctt tggttctttt ccggttgttt
    46201 atgatttcaa attgataaac cagggagatc tcatgagatc cgccgctatc tacactaagt
    46261 tcagaaatag taatatcata actgtagccg aattcctggt ttttctgttc aattaatttt
    46321 gggggtttag taaatcaaat agagttgcct acatttatta gtacctattt ccaacttagt
    46381 gtaaccctga aggcaggtac gatttaccag gtaatttaat aattttagga aggtttaatg
    46441 ctaatttgat agcgataata attgatttat ggaattatca tcgttccata gtgacctttg
    46501 catcaaatag tataactttg tatcccataa gttacagtcc aaacttatgg cttcaaccaa
    46561 atatattttc gtaaccggtg gtgttacatc ttctttaggg aaaggaatta tctcagcttc
    46621 tcttggtaaa ttattacatg caagaggctt ttcggtaact atccagaaat tcgatcctta
    46681 tattaacatt gacccgggaa ctttgaatcc ttatgaacat ggtgagtgct atgtaaccga
    46741 tgacggagct gaaacagatt tggatttggg tcattatgaa agatttttaa aggttaccac
    46801 atcacaggag aacaatgtaa ccacgggcag gatttacaac aatgtaatta caaaagaacg
    46861 caagggtgat tatttaggaa aaactgttca ggtggttcca catattaccg atgaaatcag
    46921 aactaatttt ttgaaattgg gcgagagcgg caagtatgac attgtgataa cagaaatcgg
    46981 cggatgcgta ggtgatattg aatcgcttcc ttttatagag gcggtacgtc agacaaaatg
    47041 ggagcttggt tcaaccaatg tgatttcaat tcatctaaca ttaattcctt atctgaaagc
    47101 tgcaaaagag ctgaagacaa agcccacaca acattctgtt aagcagcttc tcgaagccgg
    47161 aattcaaccg gacattttgg tgtgccggtc agaataccca ttgccaatcg acattcgtaa
    47221 aaaaattgca ctcttttgta acgttcaatt gagtgatgtg attgaagcgc tggatgcaga
    47281 aacaatttat gacgtcccta ttttaatgtc taaagagaaa cttgctgagc gcgccttaca
    47341 gaaattgaag ttgaaatccg gcactgaacc ggatttgaaa gactggaaag atttccttgg
    47401 caggcataaa aatccaacaa gtgaagtgaa tattgccctg gttggcaaat acgtggagct
    47461 tcccgatgca tacatgtcga tagcagaatc ctttgttcat gccggtgctg aaaatgaatg
    47521 cagggtaaaa attaattaca ttcatgcaga gtcgatcaca aaaaagaaat gcaaacattt
    47581 atttaaggat cttgacggca ttcttgtggc gccaggtttt ggtgaacgcg gtattaatgg
    47641 caaaattgaa gccgtccggt atgcaaggga aaacgggatt ccattcttcg gaatttgtct
    47701 tggcatgcaa tgcgcggtag tagagtttgt tcgaaatgtt atgaaaatat ctgacgccgc
    47761 ttcaacggaa attgaacccg caaccaaaca tcccgttatt gatttaatgg aggatcagaa
    47821 gaagataacc atgaaagggg gcaccatgcg attgggcgca tacaagtgta atttagttaa
    47881 aggaactaaa gtgtattcaa tatatgggaa aagtaaaata agtgaaaggc acagacacag
    47941 atacgagttt aataacaaat acttgaaaca gatggaaaag aaaggtatga ttgcatcagg
    48001 aattaacccg gaaactggtc ttgtggaaat aattgaactg aaaaatcatc cttggttcat
    48061 aggaactcaa tatcaccctg agctgaaatc aacagtcctt aaacctcatc cattgtttgt
    48121 taaatttgtt aaagcggctt tagatttcca taaaactaat ttttaaaaga tagtagatgg
    48181 ataggaatca agcaatagga ttagtattaa tttcgctgtt actcatagca tattttcaat
    48241 tctttgcacc ggaacccaaa cctcaggaag agccggctca aacagaatct gttcaaccag
    48301 caacaccgga gcaatcccaa acacaggagc ctgacaattc aagaattcaa gatcaagaaa
    48361 ctgctcctga tgacaaccag ttggcaaatc agtttggtat cttcgctccc gccgcaaaag
    48421 gtaaaagtga aatcataacg ctggaaaata accagattga gatttctttc aatagccgtg
    48481 gtgccacccc atttcaggtt tggctgaagg aatttgaaac agcctccctt gagccattaa
    48541 tattaatgga tagccttagc agtcaaaaaa gcctggtaat tcacacagga aacaaagtaa
    48601 ttgatttata taaattatat taccaggttg agaagtcagc tgacaacgat acttcaagag
    48661 tttcctttat tttaaatctt ggatcgggtc aaaccattag gcatatctac tccctgggtc
    48721 catcaggata ccagcttaaa tataagttag aaacatcagg tctggctaac ctggttgaca
    48781 acataaatcc ggagcttttg tgggttaaca atttaaaaca tctcgagaag gatataaccc
    48841 aaagccggat agtaaccacg gttacctatt atactatgcg gggagatttc gatgatctgg
    48901 gtgagcgttc tgctgatttg gaagaagaaa cgatttcgga accggtggca tgggttggat
    48961 tcaaacagca tttctttacc gaggctataa tttcaaaaaa tggatttaca cgggcagtac
    49021 tcaaaacaca ggttgatgag gcagatcctg cggtggttaa aaaggcgttt gcgggtctaa
    49081 gcctggatcg cgcacaattt ctcggcgaaa caaatgagtt tacctattac ttcggtccca
    49141 ataattacca aattcttaag aaggtgacag aaggtttctc agataatatt tacctgggat
    49201 ggccaccaat taattttgtt aataaatggc taatcatacc tatcttcaat ttccttgaaa
    49261 actatatcgg aaactacggt attataatta ttatacttgt tttggtgatc aaaattcttc
    49321 ttgcacctct ctcctacaag tcatacgtaa gtatggcaaa aacaaaagtt ctgaaacctg
    49381 aactggacga gataaaggca aagcatcctg atgatatgca aaaagctcag caggaacaaa
    49441 tgaaattgta tcagcaagta ggtgttaatc ctcttagtgg ttgcataccg ctgttattgc
    49501 aaatgccaat tctttttgcc atgttttata tgtttccaaa ttcaatcgaa ttgagacagg
    49561 aagcttttct ttgggccgat gatctttcca catatgattc catccttagt ttgccattta
    49621 atattcctgt atatggtgac catgtaagtt tgtttaccat attgatgaca gcaagtacta
    49681 tcctgtacac gtggtcaaac aaccaaatca caacggtgca aggtcccatg aaaaccttct
    49741 cataccttat gccgataata ttcatgttcg tccttaacag ttttcccgct tcattaagct
    49801 tttactattt tgtaagcaat attgtaacct tcggccagca ggcattaatc cgaagatttg
    49861 ttgatgaaga taagattaag gccattctgg atgaaaaccg gcttaagaat aagggcaaga
    49921 aaaaatcagt cttccaactt aagcttgaag aagctatgag aaccaaagaa gacgccaaaa
    49981 agaagaaaag caagtagcct tggcattctg ttctcattag tttaaattat ctttgaattc
    50041 actctttttc aatggaattg gtttattatg gtcagtcttg ctttcaggta agcctttcaa
    50101 aggccgttat cctgattgac cctttcattt ccacaaatcc cctttcaaaa aacagagaca
    50161 ttagcaatat aaatccggat tatattctcc tgacccacgg acatggagac catacctatg
    50221 atgcagaagc cattgcaaga aattcaaatg caaccattat ttcaaattat gagattacta
    50281 cctggtacga aagtaaagga attggttcaa ctcatggaat gaatattgga ggcagctatc
    50341 attttccttt tggaagatta aaaatggtaa atgccattca tagcagttca atgcccgacg
    50401 gttcctatgg cgggaatgcc gcgggttatg tgattgaaac cagtgaaaaa actttttatc
    50461 atgccggcga taccagtttt ttttccgaca tgaaattaat tggtgatgaa ttcgatctcg
    50521 attttgcctt tttgcctgtg ggtgataatt ttactatggg aattgatgac gctttgaatg
    50581 cggcatcatt gctaaataca acaacgatta ttgcaatgca ttttgataca tttccggtta
    50641 ttgaaattga tcatgcagcg tgcagagaaa aggcaagtgc aaaaaaaatc gacttaataa
    50701 ttccaaaaat tggcgaaagc ctgacagttt aattttaatt gatgggaaaa ataattgcga
    50761 tagcaaatca gaaaggggga gttggcaaaa ccacttccgc gataaacctg gcggcaagcc
    50821 tggcagcgct ggaatacaaa acacttatcg tggatgccga tccgcaagcc aactcaacat
    50881 caggaattgg ctttgatcct aaggaagtca aaaccagtat ttacgaatgt atgatcaatg
    50941 aagtatccgc ctctcacgcg gtaattgcaa ccaatttaaa ttatctgtat ttgcttccct
    51001 cccatattga cctggttgga gctgaaattg aaatggtaaa cctggataac cgggaagaga
    51061 aaatgcgaca tagcctcctt cccatagccc cggactatga tttcataatt attgattgct
    51121 ccccttccct cggccttatc acaattaatg cccttacagc cgcagactcc gtaataatac
    51181 ccgtgcagtg tgagtatttt gccctggagg gattaggtaa attgttgaac actataaaaa
    51241 ttatacaatc cagactgaat acaaaattac agattgaagg catcttgtta acgatgtatg
    51301 atacgcggct taatttgtcg aaccaggttg tggaggaagt tcggaaacat tttaagtcta
    51361 tggtatttga tacaatcatt ccaagaaatg tgaagcttag cgaagctccg agttttggat
    51421 tgccggtaat atcccatgat gttgaaagca ggggatcaat ctgctacctc aacctggccc
    51481 aggaaattat aaataaaaac ggctacgctc cggtaacaaa tggcaaacaa aaagtccagt
    51541 aaaagaacgg ccttgggcag aggacttggc gctctgttaa gcgactcctc ttcatttgag
    51601 gcagacattc ccaggaaggc accacaactt gccggaaagt caattgatga aattcaaatt
    51661 gagaaaatta aaacgaatcc atttcaaccc cgcactgatt ttgataaaga ttcattaaat
    51721 gcccttgcag aatctatcaa aaatcaggga atcattcagc ccattaccgt tagggaaatg
    51781 tcggacggaa cattccaact catctccgga gagcgaagat tgcaggcttc cagaatttcc
    51841 ggactagaca ccatcccggc tttcgttagg aaagcaaacg acactcagat gctggagatg
    51901 gccttaattg aaaatacgca acgagaagat ctaaatgcca tggaaatagc cctctcctat
    51961 caacgattac tcgatgagtg taatctgaac caggaagagt tagctcaaag ggttagtaaa
    52021 gaccggtcta ctatcaccaa ttacctgagg ctgcttaaac ttccacccga tattcaggcc
    52081 ggtctaagag ataagaaaat tagtatgggc catgcacggg cattaattac aattaacaat
    52141 gtggaagcac aactattaat ttataaaaaa ataatcagtg atgatctttc cgttagaaaa
    52201 gtggaagaac ttgcaaggct tttaaataag agaagtgatt ctaaagaaag taaaaaagga
    52261 aaaagtaagg atggagatat tgtacggatt gaatcgaaac tgtcgtcctt ttttggaacg
    52321 aaaattgaaa tagatcataa aggcaaatca ggcactatca aaattcctta cctaagcgcc
    52381 gatgatttgg aaagaattct ggaattactg aatgtacggt aaaccattcg ttatattaat
    52441 tttcattatt tgctttagcc aggctttgcg ggcacaggat acgcttgttt atgttggaga
    52501 ttccgtcgct attaattccc tcaacattga acagattaag gcaaagcgat tttttgaccc
    52561 ctcccgcgca gctcttcttt ccgcggtaat gcccggtctt ggacaggcct acaataaaaa
    52621 atattggaaa atgccaatag tgtggggggg acttgttctg ggaatctcct tggcaaattt
    52681 ttataacgag cgattgcaaa tattccgggt tgatcacttt gaacgattga atggccagcc
    52741 aggaactcta atggtttcaa caagaagatt ggagcaacta attcagcgct atagaagaga
    52801 tagagatcaa actgttatct acacatttat gtggtatggt ttaaatattg ttgatgctca
    52861 cgtggatgca tatttaaaag agtttgatgt aaatgatgaa ctggctttaa aacttgaacc
    52921 atatgtcgac tccagtgtgc cttggcaaat gaatgcggga gtttcactag tattaaaatt
    52981 caaatgaaaa taattctgat aggttacgga aaaatggcaa aagccgtaga agttgaagcg
    53041 atcaaaagaa atcatactat ttcagaaatt attttccatg gagatctgaa taaattaaaa
    53101 aaggaaagtg gtgaagtcgt tgttgagttt acaaacccca attctgcatt tgaaaatatt
    53161 cgcgaatgtc ttaatctggg cttgccggta atttccggaa caaccggatg gcttgataag
    53221 aaatctgaaa tagatgccca ttgtttaaag atggatggaa cctttttcta tgcttccaat
    53281 tttagtatag gggtaaatct ctttctcaaa atcaacaagg cagtagctgg tataatggag
    53341 agaaagggtt atcaaatctc aattgaagaa attcatcata ttcacaaaaa agatgctcca
    53401 agcggaacgg caatcagtat tgcaaataat atcctgcaga acaccacaga atataaggaa
    53461 tgggctttga atcaaaataa accgtataca attcccattg aagccattcg tgagggtgaa
    53521 gtacccggaa ctcacaaagt gagctactcc tctgatgtag acaaaattgc attaacccat
    53581 gaagcatatg gcagagaagg atttgctaaa ggagtaatta gtgctgtgga atggataagt
    53641 ggtaaaaaag gtccaatggg aatggatgat ctcttaaata tttaaactta ctttggggcc
    53701 ttcaacaata atggaattat tacgttataa ttttttaata actgaaagag aatggattta
    53761 aaattctgga agaaaaagga taaagattct gaagataaaa aaccaaaatc aaaggtaaga
    53821 gaatgggttg atgcgatcgt tttcgctgtt gtcgcggcaa ctataatcag gtggctaata
    53881 cttgaagctt ttacgatccc tactccttcc atggagaaaa gccttttggt aggagatttt
    53941 ctgtttgtaa gtaaggtaca ttatgggacc agaaccccaa aaactccgtt acagatacct
    54001 cttactcatc aaaaaatttg ggggaccaac attccctcct atttagattg gattcagtta
    54061 cctcagtacc ggcttcccgg ttttacaaat gtaaaaagag gggatgtggt agtattcaat
    54121 tatccggtag aattcaatta tcctattgat ttgaaaacga actatatcaa aagatgcgtt
    54181 ggcgcatccg gtgatacgct gcaaataatt gacaaattgg tttttgtgaa tggaaaagaa
    54241 tttgaaaatc ctcaactgat gcaatttaaa tattatgttg tctccgatga tccaatcaat
    54301 caacggatat ttgatcgata tgacattact gatatttttc aaggtcaggg attttatgta
    54361 gttgatactt caccggaaac agcaaaagaa ctggctgccc tgccattcat caaagaagtg
    54421 agactatatg aacgcaagca gggtgaatcg gaccccggaa tcttcccgga tgctgccaga
    54481 tttccatgga acgctgattt ttacggcccg atttgggtgc cgaaaaaagg agctgccatt
    54541 cagctaactc cagacaacat taccaaatat ggggatgtaa tattatacta tgagcatcat
    54601 gatgacgcca gaattgaagg tggtaaagtg tttattgacg ggaaagaaat taacagctac
    54661 acatttaagc aaaactatta ttttatgatg ggtgataacc ggcataatag tgaagacagc
    54721 cgctattggg gatttgtgcc cgaagatcat gttgtgggaa aagcattctt tatttggctc
    54781 tcattggatc ctgataaagg aatactttcc aaaatccggt ggcggcgatt ttttaatctc
    54841 attcgttaat tctaccagtc tatggatttc aggtttctgg aagcaaggta attattggtc
    54901 ttactgaaat ggttgcaccc aaaaaatcct ctatccgcgg aaaatggtga gggatgtgga
    54961 gcggaaagaa tcaaatgtct gttcttatta attacctcac ctttcttctg cgcataagag
    55021 ccccaaagaa gaaaaaccac gccttctttt ctttcagaaa ctactttgat tacctgatca
    55081 gtaaaatgtt cccagccttt attttgatga gatcccggac tatgcgccct gacagtcagc
    55141 gttgcattca ataaaagaat tccctggtca gcccatcttt ccaaatttcc gtccgccgga
    55201 atttcttttc ccaaatcatt tctaatttct ttgaaaatat taaccaggga tggtggcatt
    55261 ttgactccct tcgaaactga gaaacacaac ccatgagcct gacctggacc gtggtaagga
    55321 tcctgcccta ataacactac tcgcaaattg tcaaatgggc aatgattgaa agaattaaaa
    55381 atctcccttc ctttggggta tacaatatga cgggagtatt catctttaac aaaatccgaa
    55441 agtttattga aatattcctt ttcaaactct cctttcagca attctttcca gcttgattca
    55501 atcttaacat ccatttacgc ggccatttgt tatattcacc atcagaactt tattaatctt
    55561 agtgtgtaaa ataaacaaaa ttcctaattt tgattatggt tacccaaata acccaaggtg
    55621 tcaaggtaag tattgaaact gaatatcagc ccgagtattc cagtcccagc caatatcatt
    55681 atgtatttac ttacagggta acaatagaaa ataacagtcc atatacattg caactactcc
    55741 gtcgcaaatg gcaaataatt gatgtaacct cacttcgcag ggaagttgaa ggcgaagggg
    55801 ttgttggcca gcagccaatt cttgaacctg gccagattca ccaatatgtc tcaggatgca
    55861 acctccgttc cggaattggt aaaatgtatg gaacttatat gatggagaaa gttgtcgatg
    55921 gtttgcaggt agacgttgcg attcctgcat ttacaatgat tgcaccattc cgatctaact
    55981 aagaaacctt ggaattaggt accctgtcga gatatatcaa atactggttg aatgccgttg
    56041 aagagcattc tcttcattct ccttatgtgt atgagttata tacccaggtg attaaatcag
    56101 agtatcctga tcctgaattt gagagaatag aaaagattcg ggaagccctg gtgaatgata
    56161 atagtattat taaaatcaat gattttggat caggttccaa aatacttcat ggtaatcagc
    56221 gcactatctc acaaatcgcc agaacatcca tctcaaatcc caaactttca aagctgctct
    56281 atagattgat tcgtcagcaa gaagcaaaag aagtagtgga attggggact tcattaggca
    56341 taaatacgct ctacatgtat ttaggtaatg aacagattag gcttactact tttgagggtg
    56401 atgaaaatat tgcaaaatat gcagaacatt cattcaaaca atttggtagt gagaatattc
    56461 aactccggac tggggaatta gaaaaaactc tatctcaata tctcgcattt tcatcaaaaa
    56521 ttgactttgc atttattgac gccaaccata cgttcgaatc tacggttagg tatttcaaca
    56581 aactggctaa aaaaagtcat gatgaatccc tgtttgtaat tgcggatatc cattggtctt
    56641 ccgggatgga gcgcgcctgg aacgaaatca agaaaagtcc ctatgtaact ctcaccattg
    56701 atgtatttga agccggcctt gtattcttta agcccgatat taccaagcaa cattatattt
    56761 tggagttttg attccgctca agaatcataa ataattccgt tccttgtctg ggttttatag
    56821 aattacggca tctttcgaaa atcctgaaag caaatagtga ctcaaaatag gatttatatt
    56881 caaccaatgt gccgccaaaa ggaggataat cggcattcag gaaactatca aataatacac
    56941 ccaccagctt tccaccagga atcagcaaat caaacatttt ttcggcataa cgttgacgta
    57001 aattccgatc gaaagaacaa aaaaaagttt gttcaattat taaatcaaac tggccactta
    57061 tggtaaagaa gtctgtttga atcaaattct ctgtgggaaa ttcagtaacc cgcttgcgaa
    57121 aattttctag tggtgtgcgt gcaaaatcca aaacaaaaac attctgaaat ccactcttaa
    57181 acaagtattc tgcttcataa ccatttccag cccctggaat caaaatgcgg aggtctccat
    57241 ttgttaattg atcaacataa tcctttatgg gcgtagtaat gcttccagca tcccaacctg
    57301 tagtgccatg tatatagtga tactcccaat atgcagcgtc aagacgcatt tttaattctc
    57361 aaattgtaat tgtaatgaaa tatagggtaa ttttggccaa tcattttttc atataatgac
    57421 tgaagaaaaa aaatcaactt ccaaacgaaa tattatcata attggtataa tagcagttat
    57481 cgctattgtt aatggagtaa aatggtatct ggagtctgaa gaaagggagg agttacaagt
    57541 atattacacg aatgagctga gtgcggcgca gacccggttt gaagagataa gtgtggagct
    57601 tgatgaaaaa attgctgaga ttgaaaggct gggtggtgac attaaagaat taacagaagc
    57661 caaaatagag gttgaaaaag aaagaaacca attgcagcag acgcggaccg ctaaccggag
    57721 attaatagct gagttgagag ataagacgga gggatatgcc gaattattgc tggcaaaaga
    57781 tgaagaaatt acgaaactta aaacagtgaa tacggaattg ctttccgaaa ataccactct
    57841 taaaacagaa aaaaatgaac tcagcaagtc aataaccgaa cttaaccaga acaaagaaca
    57901 attagaggag aaggttgccg ttgcatcaag actggaggcc agcaatattg caatttatgc
    57961 ggtttcagat ggtggccgcg aaagggaagg ttcattcaga aagagacaaa tcgaaaacct
    58021 taagattgaa tttaatattg ctaaaaatga tgtggcaccc attgaaggca aagaaattct
    58081 gctcagggta atcgactcaa ataaccaggt gtattttgat gtagcccggg gagcaggaac
    58141 cttccttatt gatggaaaag aacaatttta tactctcaag caagatatac tatttgataa
    58201 tacgagtcag aaagtgacct tcttatatga aaaaggtagt gaatacaatg caggtgacca
    58261 tactgttgaa atctataccg atggctactt aatgggtaaa aaggccttca ccgtgaaata
    58321 aaagaccctt tctttgatta attttcgaat ttggctacct ttgcaagctc attttaaaac
    58381 caaaattaga agaacaaatg tttaaaaatt acgagacggt attcatttta actcccgttt
    58441 tgtctgagga tcagatgaag gatactgtcc taaagttcaa aaaggtttta gaggatgatg
    58501 gtgctaaaat tattaatgaa gaaatgtggg ggctaaaaaa actagcttat cccgttcagc
    58561 acaaatccac tggcttttac aatcttattg aatttgaggc aggaccacag acaattaaga
    58621 agttggatat tgaataccgc agagatgaaa aagtaatgcg atttcagaca gtcagtttag
    58681 ataagcatgc tattgcttac aatgaaaagc gaagaaaagg tgcattcaac aagaaaagtg
    58741 aagttaagga ggagccggta ttatgacctt aatgaatgaa cctttaaaca gggtagaaaa
    58801 ccgaagcaaa tattgcaggt tcaagaagag cggaatcaaa tatatcgatt acaagaatcc
    58861 tgactttctt ttgaaatttg ttaatgacca gggaaagatt cttccccgga gattaaccgg
    58921 aacaagtctg aaattccaga aaaaggtatc acaggcagta aagagagcca ggcacttagc
    58981 ccttcttcca tacgtaaccg attcattaaa gtaaaccagc ctaaagaaaa tgagagttat
    59041 tttaaaagac gacatcaaag ggttgggata taagaatgat atcgtgaccg ttaaaggagg
    59101 atatggcaga aattacctga tccccaatgg ctttgcaatt gtagccaaca aatcaaattc
    59161 aaagatgatt gctgaaaaca ttcggcaggc cgcgcataaa gctgaaaaaa ttaaaaaaga
    59221 tgcagaagaa ctggctggta agattggaga aatgacaatt gaacttggag caaaagtcgg
    59281 tgaaaaagga aaaattttcg gaagtattac agccatccag gtatctgacg cattaaaaga
    59341 taaaggattt aatattgata gaaaagatat tacattcaaa tcacaaataa aggaagtagg
    59401 tgattacgaa ataatacttg atttgcacaa agaagtaaaa cacagtatta cagtaaaagt
    59461 agcagccgaa taaagttaca gaaaaaaata aattataagc cgcttcaatg gaagcggctt
    59521 ttttgtttgt attcattttc agtaacttta atcatgttca gaacggaact taatttcccg
    59581 ccttcaaccg agaagattgg ccttaaagac actatcgtaa gcctaggatc ttgctttgcc
    59641 gataaccatg gcgaacggct catcctgaat aaactatcat gcctggttaa cccggcagga
    59701 ataatttata acccttactc catctgcaaa gtgatagatt atgcgctcaa tgaagaattg
    59761 cctgatcagg catcatatct tcagcatgat ggagtttttt caaactttga tttccactcc
    59821 aggatatccg acaccgatgg aatcaaactt cagcaaaaaa tctcccactc actccatcaa
    59881 cttcagaaga acctggtaag ggcaaaatgg ttaattatta cttttggaac cgcagtagtc
    59941 tacacaagaa aggataacaa aataattgtt gccaactgtc ataaagttcc tgcatcaaaa
    60001 ttcaacaaag gaatgttgtc acaaaagcaa attcttgctg tatttatgca tacttaccaa
    60061 tcaattatgg cagcaaatcc ggaaattcag attcttctga cagttagccc ggtaagacat
    60121 gttagggata cgctcgaaat aaattcaatc agtaaagcaa tattaagaat cactagtgat
    60181 actttgaaaa ctgagttgcc caacattcat tattttccat cctatgagat aatgatggat
    60241 gatctccggg actatcgatt ctatgccgat gatttgattc atccatctca tcaggcaatg
    60301 aattatatat gggaaaaatt ttgtaaaggt tttatggatg atgctttact tgactttatt
    60361 aataaatggg gtgaaatcag gaaagatcta aaccatcgtc cttttcatcc tgaaacaaaa
    60421 tctcacctgc aattccttac taacaccttg gaaaaactca aacaactttc tggtgaagta
    60481 aatttagaag ttgaaattgc ccaactggaa aagcaattga atgacaaata agaaagcaaa
    60541 caggttaata aaatcaaaca gtccttactt gcttcagcat gcctacaatc ctgttgaatg
    60601 gtttccctgg gacaaagaag ctcacaaaat atctaaagaa acgggcaaac caattcttct
    60661 cagtatcggc tactcttcgt gccattggtg ccatgttatg gagagagagt cctttgaaaa
    60721 tgaaactatt gccagggcga tgaatgaaaa gttcatctgt ataaaagtgg accgggagga
    60781 gaggcctgac atcgatcaga tatatatgga agctgttcag gcaatgggcg gtcatggtgg
    60841 ttggccgctt actatctttc ttacaccgga gttaaaacct atttatggcg gcacctattt
    60901 tccaccggaa aatttcctgg tactgctgga gaaggtaagt tcggtttatg caaacaacaa
    60961 agcggagatg gattcttccg ccaaccagat tgcaaccgcg cttacaggat ctgaacttga
    61021 taaattcaga cttagataca atgatgaagt tagtactttg aaacttcaaa aaatgtatca
    61081 aaatctctct tcaaaatttg ataaagaatt tggaggaatg tcgagggctc cgaaatttcc
    61141 aatgccggga acctggaagt ttctttctca ttttgctttt atttttaaaa ataatgaagc
    61201 tctgcaccat ttcttcttta cgctcgacaa aatttcagat ggtggaattt acgaccaggc
    61261 gggcggtgga tttgccaggt atagtacaga tagaaaatgg catgttcctc atttcgaaaa
    61321 aatgctttat gataatggtc agttaatgtc actgtatgca atagcgtatt ccctttccca
    61381 aattgacagg tacaaagaaa ttctttatga aacccttgca tggctcagcc gggaaatgac
    61441 tcacggtagc ggcggctttt actcggctct tgatgcagat agtgaaggcg ttgaaggtaa
    61501 attctacgtg tggaaatggg aggagtttga aagaatagca aataaaaata ctaacaaatt
    61561 tgcggcattt ttcggagtca ttcctgaagg caattgggaa gggacaaata tattactaag
    61621 aaatcagggt gtgcctaagg aagatcagga agaaatagaa gaattcaaat cgcgacttct
    61681 gaaagaaaga aataaaagaa taagaccagg attggatgat aaaattctgg caggatggaa
    61741 tgcgctaatg tcatcagggt tgcttgatgc ttatgatgca accggtgatg aagtattttt
    61801 gcaacacgct gttaagaacg gcactttcct gatgaatgag ctattcattg atggcaaatt
    61861 attccgctca tggaaggacg ggaagccagg tacagccggt tttctggaag actattcctg
    61921 cgcaattgag atgtacctga aattatatca atctacattt acccggaagt ggctggattt
    61981 cgctttgcag cttgtgaaga ttgcatatga gaagttttat gatccggcag atggcttttt
    62041 ctatttttcg gaacagtctt cggatttgat cgcgagaaag aaagagattc ttgacaatgt
    62101 aatcccggct tcaaactcga ttatggctaa cgccctgttt aagctgggag cattaatggg
    62161 ggatggaaaa tatatcgcgt tggccaaaaa aatgactttt agtttgattg acctaatgga
    62221 atctgagaca gaatatacct caaactgggc gctcctatac agttatcttg tttatccaac
    62281 ctccgaagtg gcacttgtcg gaaaggattt tgaaaaatta ggaaaggccg tcagaaagga
    62341 gtatttcccg aataaggtaa ttgcaggcac gaaagcgaaa agccaaattc cccttctagc
    62401 caacagggaa acaagtagta aaaattcaat gatttatgtg tgtcaaaacc acgcatgtca
    62461 acttcctgtt actaatcctg atgaagcgtt ggaacaaatg aaagataatt taaaaataat
    62521 caaatcgcac tcctgatttg tgaagaagtt tcagctaaat atggatgcgc cggaagagag
    62581 agaaacactt tttgctgaca ttattctacc agtacctgtt cccggtcttt ttacttatcg
    62641 catccctttc cttttaaata atcaaatttc gattggatca cgggtaattg ttccttttgg
    62701 cagcaggaaa atccttacag gtgtaatcag gaagattcac aatctgcctc ccgcaaagta
    62761 tgaagcgaaa cttatacatg atgtgctgga agaaactccc tctatcaata attttcaatt
    62821 caaacttttt gaatggatag cgagttacta catgtgcaca attggtgaag taataaatgt
    62881 tgccatcccc tcaggattaa aactaagcag tgaatctctg gttcagcttc atccggagtt
    62941 tgacagagac ctttctgact atgaattttc tgaaaaagag atttccgtct taaatgcatt
    63001 gtctaccgac cgctctctac cttatgacag gattcacgaa ctaacggata caaaaaatat
    63061 tcattccatc ataaaaagcc tggtaaggaa agacgcgatt ctgatttatg aaagcataaa
    63121 agagaaatac aaacctaaga aaataaaaaa aataagattg acaggtgaac acctggatga
    63181 gaaagtgttg gagaaattaa tgaagtccct ggagaagaag ggtaaacaat tggatattgt
    63241 tttgatgtac ctgaaaacag tggatatttt tgaatctccc gaacttaatg ttttaggaat
    63301 gaacaagtct gatttaccac aatcctcttc ccttcaaact ttgataaaaa ataagatttt
    63361 cgatgaattt gaagtaacgg ttccccgact ggagaccttc tccaaagatt tgaaaaatgt
    63421 gattcttaac gacaatcaac aaaaagtttt ggatctaata ttacaagagt ttgaaagcaa
    63481 ggatactgtg ctccttcacg gaattaccgg cagcggcaag acagaaattt atattcaatt
    63541 gatcaaagaa gcgcttgata atggtagtca ggttttatac ctgctcccgg aaattgcttt
    63601 aacaacacaa attgtaagcc ggctaagaaa agtatttggt gataagatgg gagtttacca
    63661 ctcaagattt tctgataatg aaagagtgga ggtgtggaag ggaatccagg atggaaagtt
    63721 ttcttttatt gttggagtca gaagttctgt tttccttccc ttttcatccc ttggactggt
    63781 aatagtggat gaagaacatg aattatctta caagcagttt gacccggctc cgaggtataa
    63841 tgcaagggac accgcaatag ttctggccgg atttcacaaa tcgaaaattc ttttaggctc
    63901 cgctacacct tctattgaat catattttaa tacgaagcat agaagctacg gactcgtaac
    63961 tttgtcagaa agatttacca atacaccgct tccggaaata attcctgtaa acatgattcg
    64021 gcaaagaaag aaaaagcttg tacacggaga gttctcccag cctcttttcg acgcaattgg
    64081 ccaggccctc gcggctaaag agcaggtaat tatttttcaa aacagaagag gatatgcacc
    64141 gcatcttgtt tgtgaggatt gtggatggat acccaaatgt aaaaattgca cggtttcgct
    64201 aacctaccat caatacagca aagatctccg gtgccattat tgcggatata aggagaatat
    64261 tgtaatgcaa tgccctgtat gcggttcacg atcattacat cctgttaaat atggtactga
    64321 gaaactggaa gaagatttgc gcatgctatt tcagaacgct aaagttcagc gcatggacct
    64381 ggatacaacc cgcaagaaga aaagttatga gcaaataatt gaagattttg aaacgggaga
    64441 aattgatata ttagtgggaa cacaaatggt taccaaagga ttggatttcg atggagtaac
    64501 cctggtgggt attctggata ttgaccggat gatgcatttt cctgatttca gatcttatga
    64561 gcgaacattt caattagcta cgcaagttag cggccgcgca ggcaggcgtg ataaaaaagg
    64621 actggttatt attcaaacta ataataccga acatcccctg atgaacaaga ttgtaaatca
    64681 caattataaa ggaatggtga atgttgaaat taatgaaaga aaaagattcc gatttccgcc
    64741 ctttgtcagg cttattaaaa taattgtccg ccatgacgag ttaattattt gtgaaaaggc
    64801 agcaaaagct ctatctataa gattaattca ggaacttggt cttcaaagga ttcttggacc
    64861 agccgaaccg atgatatcaa aaataagaaa caaatacctg agagaaattt atattaaatt
    64921 agagcgcgat aaagtgaatc ttgataaagt gaagaaaaat attctggaga taggacgcga
    64981 aattcaaaat aacaaagact ttaaaaaaac cagtctgatt tttgatgtag acccatactg
    65041 atgaaaatat tcaaaattgc tgctgtcatt ttttttgttt tctttctgac cgcttgtaag
    65101 gaagatgata ctcctttgga tttaactcag tatgagcctt ttgttaacat tggcttttat
    65161 gatacgctaa atcaaccggt ggaaactctc attgaacagg ttcaaccgct tggtcataat
    65221 ccatttacct ttccagaacc tgccacaaca ttccggttcc ctctggatat taatacgaca
    65281 atgacgaaat ttgatattac catttcggaa gaaacgggta ctattactgt agaatattta
    65341 cgcagggttg aaaatcaaat taacattctt cggtttatta tggaaaaaat tgttgtttca
    65401 gctcactcat atgattcggt gaaaatagta tgtgaaaccc cttgcatctc aaatgaaact
    65461 gaaatcagga tttacaggta gtattctgct attattaatt gcagccgaat cttttggaca
    65521 aacggattcc ataaagaatc atttctctgt tgacttgagt attgattacc tgaaattcgc
    65581 tagttacctt tcagattcac aagaaaaata cgaaggtgag ttgggtatca tgtttgggcg
    65641 gtacagaatt gccggagaat acggaatggc ggagttaacg ccaaaggatt tattcgacaa
    65701 tgcagattat tacgccaaag gaaattattg gagaatcgga ttcgatttca aattacctgt
    65761 aaatccaaag aacaatttat atatcggagc ccggtatgca gccactgagt ttgatgaaag
    65821 tatcctgatt tattacgata gtaatacttt caacccgtat ttcgaagaaa acatgggttt
    65881 gaaagcaaac tggtatgaag ctgtaatatc ttccgaatcc cggataattg cacaactata
    65941 tttgggattt aaattgagag tacgttttat ggattcttat gataatcccg gactcgtgga
    66001 aatatattcc attcccggct atggaaaatc ctttgataga tctgcgcttg ccgtgaatct
    66061 tttcgccaga taccggatta ctttctggta attatttccc actcttcatt aagaaatgca
    66121 atgccgtgat gagcggttaa atcatacata caaggaacga tggagacaaa attatttgct
    66181 atcgcccatt catcattatc ttcaccttta tcaaaattta cgaaattacc ggccatccag
    66241 aaatatcgtc ttccgtttgg atcatagcgc aaatcaaact cttcctgcca tttggcccgc
    66301 gcttgccggc aaatctttat tccttttata ttttcatttc tttttggagg aaaattaaca
    66361 ttaagtgcaa ctcctttcgg taatcctgtt ctcaagacct cttcagtaat ttcttttaca
    66421 taccttctgc aatgagaaaa atcagcagtt gaagaataat cacacaaaga aaaaccaatg
    66481 cttggaaatc cctcaattgc gccttcaata gcggccgaca tagtacccga atataataca
    66541 cttatgctgg tattacttcc atgattaatt ccgcttacta caacatcggg taagcgatca
    66601 tttaaaacga aatgtttggc cagtttcaca cagtccgccg gagttccgga gcattcatag
    66661 gcaggtatat ctccaaaaat atccgtcgga tcgagtctta atgtattgcc caccgttatc
    66721 gcgtgacccg tccctgactg aggcccatca ggtgcaacaa caacaacctc ccctatttcg
    66781 ctcattatat taaccagatt tctgattccc atggaggtaa ttccgtcatc attcgaaatt
    66841 aatatcagcg ggcgtttctt ttccattccc aaaagtaagg aaaaggatta aatggtggat
    66901 aatttaattt tccctgttaa tcagccgtca agcagggcat tgtaataggc aaagattcgg
    66961 gcaagatccg atttctcatc ggtcttaaga ttgttctttc taatatattc tttgatctcc
    67021 cgctgcctgt cttttagtaa atcaaggagg tctctgtatt tcttactgaa atattgtatc
    67081 tccccgttct ttcgtaagat aaaatagtca tagctgagaa cctgacgcgt ataagtaccg
    67141 cccgaccagt aataagaatt ataatgggaa gtttcattca caatggattc cctggctaaa
    67201 agagttacct tgccttcata aataacttca aacaatgttg gagtcttata atttgattta
    67261 agatcatagg gaatggaata aaactgacgg taattctcta tagtgacatc aaaaatttca
    67321 aaataaaaaa tctttctgga actgtaagtc atcaggcgcc cttcaccatt aatctgcaca
    67381 aggtcagttt caatattgta ttttatttca ccggaaattg tatcgccact aattagtacc
    67441 atcttcccag gatgccagaa ttctgatgga aaatcctgcg ctggcgaaag ggaaccaatc
    67501 aggcaaaatc cgattaggag tcccgctatc ttattcatgg ctattttttc aaaatagtat
    67561 gccccatttt atcgcgcttg gtggtcaaat atttttcatt atgctgatta ggagcaatct
    67621 ctataggtac attttctaca atttccaggc catacccgat tagtcctgct cttttagtag
    67681 ggttgttaga tataagtctg attttagtaa ttcccagatc tcttaggatt tgcgcgccaa
    67741 ccccataatc tcgttgatcc attttaaaac ccaattccaa attcgcttcc acggtatcaa
    67801 gtccttcttc ctgaagttta taagctttca atttattcaa aagaccaatt cctcttcctt
    67861 cctgattcat gtataagaca acccccttcc cttcacgctc aacaatttcc attgctttat
    67921 gcaactggtt gccgcaatcg cacctgcacg aaccaaaaat atctccagta gcacaggatg
    67981 aatgaactcg caccagaacc ggctcatcac ttttccagga acctttcgtc agtgcaagat
    68041 gaatttcatt ggtgctcgtt tgtctgtagg ctgtaagatg gaaatccccg aaagtcgttg
    68101 gcatatccac atcaatttcc ttcttaatca gtgtttcatt ggccagcctg tatgcaataa
    68161 gatcttttat tgaaaccagt ttcaggctaa acttattggc aactcttctt aaatctggta
    68221 acctggccat ggtaccgtct tcgttcatga tctccactaa cactcctgcc ggtttgaaac
    68281 cggccaacct ggcaaagtca attgcagctt ctgtgtgtcc ggttctccgg agcactccgc
    68341 ccttctttgc acgcaaggga aaaatgtgac ccggcttgcc cagatcctcc ggtttaattt
    68401 ccggatcaat cagtgcacgc actgtttttg atctgtcgct ggctgatatt cctgtggtac
    68461 agccatgacc aaggagatct acgcttacag taaatggagt ttgatgcaat gccgtattcc
    68521 ttcctaccat cagctcaaga cctaattcct cgcatcgctc ttcaataaga ggagcacaaa
    68581 taagtcccct tccatgtgtg gccatgaaat ttataatgtc aggcgtgacg cattcagccg
    68641 cacaaataaa atcaccttca ttttccctgt cttcatcatc tactacaata atcacttcac
    68701 cattttgaat tgcttcaatg gccgcctcaa tcttatctaa cttaatgtca ctcatcaaag
    68761 tctgaaattt tatcaaaaat acgaagattt tccaggttat tgaaattcac aggcttatgt
    68821 ctcaatcatt tactacaatg cccaaaagat tcccaatttc tctctccgcg gactttaacc
    68881 ttgcaatatc tttctgtagg ttctcctgct tgccagggtc tggctcatcc ttaaggtttt
    68941 tgagtttatc tcttaataac tttccgttga gcctgaattt taatcgaaga atgttatgat
    69001 agaaaagatt aggcagtaat tcgctttctt cagggactgg aattttatgc ccaaaccaat
    69061 tagggctcac ctcatatcta agcgttgtta aatccaccac ttctcgctga acttcctcat
    69121 tgccgttagt cataaggtat tgcatatcaa ttaaattacc tttagctaat tgttctttat
    69181 aaatatccag aatctcttta taaaccggtg tcttaaattc aatttcatcc agttctgata
    69241 agaagtagtc ataggcttta tattcttttt ccagttccga atgaccgtaa gtaatcagca
    69301 atcggattcc ttcacgttcc tgaaaacgaa ttttatcatc ctgggaaata ctttcatcct
    69361 ggttatggat atccggatac tgcgtcggct caacatcctg attagaatcc aaccgctttg
    69421 atttatccct gcgttcccga atgagaatct tattttgctc tgcaactaaa atgctttcat
    69481 ccaggtctaa ttttttggca gtctctttta tgtacacagc cctttttacg ggatcaggaa
    69541 ttttagtaat acttacaaca atctccctga taacttcagc tttcttaacg ggattattgg
    69601 caaattcctg ggcataaagt tccgtcttga aagaaataaa atcccggcta tcactcgcca
    69661 ggagttcctt catctcattg gaacccaatt tcttgacaag gctgtcagga tcatcttcat
    69721 caggcaaaac cacaatcctt acattaagac ctccctccag cagcatatcc aggcctctta
    69781 acgaagcttt caaaccggca gaatcaccat catacaaaat ggtgacattc tccgtgtatc
    69841 tttttattag cgttatctgc tctttggtca gagcagttcc ggaggatgac acaacattgc
    69901 c
//
LOCUS       DASWHX010000109        15491 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_3066820, whole genome shotgun sequence.
ACCESSION   DASWHX010000109 DASWHX010000000
VERSION     DASWHX010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 15491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15491
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3066820"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1135)
                     /locus_tag="VGA21_07550"
     CDS             complement(<1..1135)
                     /locus_tag="VGA21_07550"
                     /inference="COORDINATES: protein motif:HMM:NF025486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative porin"
                     /protein_id="HEX9650398.1"
                     /translation="MFKKLLYLSGLIVVSLELPAQVRSQIIDDSTKLVYGPKSTLSIR
                     EEDVFYQRDNWKTVDTLLTDFHKFTFLEKRDLKWTDLGVIGTAMRPVYHQPEGDIGAR
                     PGFRGFDNYFKAPKDFKYFDTKSPYIEFSLMLGGKGRSITNVLVSRNVIPQLNFGLHI
                     QKLTVDKQIGNVIEGDRSVKGTSYDAFGSFHNKKKTYWIMANYSRTRHVIFETGGVDE
                     GSIGAFRDYFDDDAEPVMIGPESIEKRRNIHSYQELQLIKPVRMYHKFDRYYQKNSFS
                     YVNDNTGPAEFFDNFYVDTVATADESHFNYYKNEGGLKGSSGKLFYNVYYKNRLIDFD
                     YKYIDSLALDFITKRPEHYIGGQVIYNLDSVADAKASMEYLLEG"
     gene            complement(1156..2217)
                     /gene="lpxK"
                     /locus_tag="VGA21_07555"
     CDS             complement(1156..2217)
                     /gene="lpxK"
                     /locus_tag="VGA21_07555"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HEX9650399.1"
                     /translation="MQYLIRFIRLLLFPVTLIYDLVTRLRNYLYDIDYTRSFQFETMV
                     INVGNLSMGGTGKTPMVEYLIKSLKTNFKITVLSRGYKRKSYGFRIANKNDDAKTIGD
                     EPFQFFQKYSDEIKIVVSEDRVYAIPNILFEFPDTEIVILDDGFQQRGIIPDLSILLT
                     EYDKPFFKDLILPTGNLRESRTGARRADVIVVTKCPDELEQIDMDSYVKKIEKYANAK
                     PVYFSFIRYLDPKPVLSSYEGKITPGVIIFSGLANPDPLRLFVTSKFDVLDYVVFSDH
                     HIYTFKDLENLISMLDGKSNISLLTTEKDRAKLISSKFYSLLEGVPLFYLPIEIDFVG
                     NGRKFDKIILDAVKRNEFT"
     gene            2260..3357
                     /locus_tag="VGA21_07560"
     CDS             2260..3357
                     /locus_tag="VGA21_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009578120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEX9650400.1"
                     /translation="MVKIKEVSDYLESIAPRSLQESYDNSGLLVGNPKTNVSGILVSL
                     DCTEEVIKEAKKRKCNLVVCHHPVIFKGIKSITGRNYVERTVMSAIKSDIAIYAIHTN
                     LDNVSTGVNKKISDKIGLINTQILAPKNDNLSKLETFVPPKWRKKVLQAIHNAGAGRI
                     GNYSECSFILSGSGTFLPNEKAKPKLGKKNQLETVEEERIEVIFPTELKRSIISALQY
                     AHPYEEVAYYLHDLQNWNEDTGSGMIGDLPVPVSSGAFLLQLKKKFGLKILRHTAFIK
                     SKVRRIAVCGGAGSFLISRAKAVKADVFVTADIKYHEFFDAEGEILIVDIGHYESEVF
                     TKDLLCTLLRKKFSNFAVNLSKTNTNPVNYL"
     gene            3367..4131
                     /locus_tag="VGA21_07565"
     CDS             3367..4131
                     /locus_tag="VGA21_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018473131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HEX9650401.1"
                     /translation="MENNVAKKLDALTHLQDIDSKLDSIIKVRGALPDEVRDLEDEIA
                     GYETRLEKINQEIKERETDISDKKLAIKECQKLIKKYQDQQMNVRNNREYDAITKEME
                     LQELEIQISEKRIKEAHAKIEAKNVDIAEVTEIMNERNKDLKSKKNELSSLTSESEEE
                     EKKLLKEKEKAEKSVEERLLLSYKKIVANARNGLGVVSVKRGACGGCFNVVPPQRQAD
                     IKERKKIIVCEHCGRILADVEGIAGEDEKKPVRRRK"
     gene            complement(4155..4778)
                     /locus_tag="VGA21_07570"
     CDS             complement(4155..4778)
                     /locus_tag="VGA21_07570"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015027483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX9650402.1"
                     /translation="MSIKKIYLVRHGETEQNRQGIVQGSGIDSSLNETGYNQARLFFE
                     KYKDFSFDKVYTSALQRSVQSMELFIDSGVPHEKLAGLNEIHWGESEGKKFGGKNDKY
                     YRELVGKWKSGMLDYPLPGGESPSDLKFRLSKALLYIMSKNDERQVLICMHGRAIRAL
                     LCIMLNYPLSRMEIFEHTNFSLYQLLFSGSMFRIETYNETLKSNKRI"
     gene            4825..5247
                     /locus_tag="VGA21_07575"
     CDS             4825..5247
                     /locus_tag="VGA21_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019597276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold thioesterase"
                     /protein_id="HEX9650403.1"
                     /translation="MFKEELTVDFLNNFCKNTMVDNLGIEFTEIGDDYMIARMPVDNR
                     TIQPLGLLHGGASIALAETLGSVAATFILNTETHYCVGLEINGNHIKAIKSGYVFGKT
                     TPIHIGSKTQVWQIRITNEKEQLVSICRITLAVIEKRK"
     gene            5249..6442
                     /locus_tag="VGA21_07580"
     CDS             5249..6442
                     /locus_tag="VGA21_07580"
                     /inference="COORDINATES: protein motif:HMM:NF012640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HEX9650404.1"
                     /translation="MTEKEKATFISSTLSSLDKKELLHRLLGKSISDNFSIAIWRMPN
                     QNSSQLFIDFKQNREFEIEELEDLPAGFVFAPFLENDKKIYIQGELQIAFSDSSIKLN
                     SLALTEDNLTWLEEALQYKSDIRIKNKFNSKSTSKSKYKSWVNEGVSRIMNGEFKKVV
                     PARILVKEWRGNPIEIFNDLSCRYPNAFVSITCTPQAGCWIGATPELLLSVDKNKIFR
                     TASLAGTKAFDQSVMLHNVTWTQKEIEEQAMVSRYIINCFKTIRLREFDEEGPMTIKA
                     GNLVHLKTNFIVDMKSTNFPNLGSTMLRLLHPTSAVCGMPKDAAADFLEKIENFDRTY
                     FSGYLGPINVEDETNIFVNIRCARLIENHSIIFAGAGVTSYSNAESEWEETELKCKTL
                     LDVMS"
     gene            6412..8166
                     /gene="menD"
                     /locus_tag="VGA21_07585"
     CDS             6412..8166
                     /gene="menD"
                     /locus_tag="VGA21_07585"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HEX9650405.1"
                     /translation="MQNAAGRNVIISNPMHLQPISNIAEIFYRKNITHVVISPGSRNA
                     PLTLAFARHPEIEVHVIPDERSAGFIALGISQNLKQPVGVVCTSGSAAINLFPAIAEA
                     YLQEAPLIIMTADRPPEWIDQWDGQTIYQQGLYGIHVKRSYTLPVDVLNKESETLLYR
                     QISEGLNFAGALPNGPVHFNFPFREPFYPDKNEKIEYSKGLKIIDSENPECSISTSKL
                     EHLSSLWKSSAKKLILVGQNDHSENLLSVLQFVAAKHKIPLVGDVLSNIHHIENSIRY
                     SDIFLSGNKEKQSSLTPDLLITIGKSVISKNLKLFLRTSQIKNHWQVQWGQTIKDPFN
                     SITEIIIVDPVLFLKEMELLSGRDDFIKQKQSNFQELWHLEEHNAKKFLKSFFPQKKF
                     GEWEATKEILGSLPQNSILHLANSMPVRWANFSGLQHFQRIDVYCNRGTSGIDGCTST
                     TMGFAITSQKPVVLITGDMAFLYDRNAFWHNYPYPLLRIVVLNNHGGGIFRLITGPDD
                     QPELEEFFETKQVLKAESVAKEFNIDYHYAGSAEQLAMVWKEFFNFDGKPKLLEIETK
                     GVTNQEIFRKFKEETEEL"
     gene            8175..8993
                     /gene="menB"
                     /locus_tag="VGA21_07590"
     CDS             8175..8993
                     /gene="menB"
                     /locus_tag="VGA21_07590"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012926348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HEX9650406.1"
                     /translation="MKYDWKPIKPYKEILFQSYNGIARISINRPDVHNAFTPLTVNEM
                     IDAITICRESPDLKVVILTGEGGQSFCSGGDQSVRGHGGYVGGDQVPRLNVLDLQKLI
                     RSIPKAVIAAVAGWAIGGGHVLHVVCDLTIAADNAKFGQTGPKVGSFDGGFGSSYLAR
                     IVGQKKAREMWYLCDQYDAVEADKMGLVNKVVPLNRLEETYVEWAEKIMEKSPIAIRM
                     LKSAFNAELDGQAGIQELAGNATLLYYMSDEAKEGKNAFLEKRKPDFDKFPKFP"
     gene            9000..10082
                     /locus_tag="VGA21_07595"
     CDS             9000..10082
                     /locus_tag="VGA21_07595"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX9650407.1"
                     /translation="MNKIFYRGKKYDLSKTGEREDLVRLSEFSRNVTELATKWLSGGK
                     EFSFQTSGSTGEFKEISHSREKIQKSAKLTISYFNLNKDDNALLVLDPTRIAGAMMII
                     RSLVAGMDLYAIDPTSDLTSFSPIFRDCNFAAIVPLQLEKLLLSKEVSEIFRNYKAVL
                     VGGATVNYAMIEKLKGMALPFYETFGMTETVTHFALRKLSGSDTNPFEVIGNHIVRQD
                     SHGCMIVEGDLTVNQPLSTHDIIQIRDERHFEWVGRSDFIINTGGIKISPEELERKIA
                     GKIYAFGIRNRFIISSVHDESLGEKIILVFEGTTISDHIISELMKTIKEDLDKYECPK
                     EIRYIEKFPEAAISKINRRAVISILA"
     gene            complement(10063..10848)
                     /locus_tag="VGA21_07600"
     CDS             complement(10063..10848)
                     /locus_tag="VGA21_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017731421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HEX9650408.1"
                     /translation="MSLISENIKFLRKKNGMTQDDLASELGIKRSLIGAYEEGRADPR
                     IENLTKIANAFRIAIDSLITSDLTVTDEKFTYRKTDKAPLRILSITLDKENKENIHLV
                     PQKAAAGYLNGYADPEYISELPRFQLPMLPQNASYRAFEISGDSMLPLESGTVVVGQY
                     VETLDSIKDGKTYVLVTGKEGVVFKRIFNYVKDKGKLFLVSDNKSYSSYDIPAEDVLE
                     IWEAKAYISVKFPDPNDNQNVSIESLASIVMELKDEVAKLKSK"
     gene            complement(10876..11163)
                     /locus_tag="VGA21_07605"
     CDS             complement(10876..11163)
                     /locus_tag="VGA21_07605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650409.1"
                     /translation="MNMISVNADDNFRLKLCSKKLHSGRHQVKFEVKNAAQKRWYGYL
                     LAEPEATFNDVLSKIQQKINAIQERDEYYHGHLYSMAVRRNREDDIMIFEK"
     gene            11256..12455
                     /gene="dinB"
                     /locus_tag="VGA21_07610"
     CDS             11256..12455
                     /gene="dinB"
                     /locus_tag="VGA21_07610"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009055695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEX9650410.1"
                     /translation="MLFSHPDRAIIHMDLDTFFVSVERLRNSELNGKPLVIGGHSRRG
                     VVASCSYEARAFGIHSAMPIGLAKQLCPDVLIISGDLEAYSKYSSIITEIIREESPLF
                     EKASIDEFYIDITGMDTFYNSYQWGTELRQKIIRETGLPISMGLSVNKLVSKVATNEC
                     KPNGQSHIQKGLEREFLAPLAIQKIPMIGDKTTQFLTHMGIRTVRTLREMPLKILQST
                     FGKIGISLWKKANAMDDAPVVAYSDRKSISTECTFESDTIDVKKLKTIIMAMVEKLAY
                     KLREEKKLTACIAVKIRYSNFDTVSKQRHLPFTSSDQALIRVAMELFDQLYSRRLLIR
                     LVGVRLSNLVYGNYQINLFDDTEEEIRLYQAMDRLKLKYGPDTIFRAATQNVNHRIRL
                     DSNLFKG"
     gene            12585..>15491
                     /gene="dnaE"
                     /locus_tag="VGA21_07615"
     CDS             12585..>15491
                     /gene="dnaE"
                     /locus_tag="VGA21_07615"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015026226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEX9650411.1"
                     /translation="MYLNCHSWFSFKYGTLSPEDLFEEAQRCGIKKLALTDINNTCGY
                     IEMLRICNEKRKIYNLEIIPGIEFRRDNQLIYIAIPYDNAGFEEINRFLSYHNNTEKP
                     LPHHAPEFSNAFIIFPWKNAPEFLRENEFIGIQPHEINKMVSSSLEKMLHKLVALYPV
                     TVKNKAGYNTHRLLRAVDQNVVLSKLSPELQGRPDEIMINESELLLRYARYPELVRRS
                     SEILNQCSFSLEFYTDKNKKTFTESKESDRSLLESLALEGLSKRYNSGFKPKARLYHE
                     LEIISRRSFESYFLIAHDIISFAKHKNFAFVGRGSGANSITAYCLGLTQVDPLELDLY
                     FERFLNPERSSPPDFDIDFSWKDRDEVTTYIFNRYHKDHTALLGTHTTFKDRSIIREL
                     GKVFGLPKPEIDNLVTYPDTYRHKDNITEMIFKYGERMLSIPNNLSIHAGGVLITEKP
                     IYAYTATDLPPKGYPTSHFEMHNAEDIGIYKLDILSQRGLGHIKDTIATVKSNQGIDI
                     DINRFQDFKKDADIKKLLSKGHTMGCFYVESPAMRQLLGKLECQDYITLVAASSIIRP
                     GVARSGMMRAYIYRHHHPNDYQSIHPKMDELMKETYGVMVYQEDVIKVAHHFGGLTLS
                     EADILRRGMSGKFRSREEFKRVEVKFFSNCKERGYADEVTARVWYEIESFSGYSFSKG
                     HSASYAVESYQSLFLKAHYPLEFMVGVINNFGGFYRTEFYFHEARMNGATIEAPCVNN
                     SCYLTSITGTCIWMGFIHLKSLEIKVAGQIEEERNKKGKFRSLDNFIRRIPIGLEQVS
                     ILIRIGAFRFTGKTKRELLWEAHLYFGKVNKNEFAEASNELFETEPMQYKLPQLEPAP
                     FEDAFDELELLGFPLYHPFDLLESKPRAEAKAEDLKKKTNQKLEIIGYLITIKYTSTK
                     NNLPMQFGTFYDEDGEVFDTTHFPGSARKFPFRGKGFYRIKGKVVEDFGYPMIEV"
BASE COUNT         4861 a         2999 c         3158 g         4473 t
ORIGIN      
        1 tgccttccaa taagtattcc atagatgcct ttgcatctgc aacactatct aaattataga
       61 taacctgacc cccgatgtaa tgctccggcc tctttgttat gaaatcaaga gctaaggagt
      121 caatatattt atagtcaaaa tcaattaatc tatttttgta atagacatta taaaataatt
      181 ttccagaact accttttaag ccaccttcat ttttgtaata gttaaagtgc gactcatccg
      241 cggtagcaac tgtatcgaca tagaagttgt caaaaaattc agccgggcct gtgttatcat
      301 taacatagct aaaggaattc ttctggtaat acctgtcaaa tttatgatac atcctaaccg
      361 gcttaatcaa ttgcaattcc tggtaactgt gaatattcct cctcttttca atactttccg
      421 ggccaatcat aacgggctca gcatcgtcgt caaaataatc ccggaaagca ccgatactac
      481 cttcgtctac tccgccggtt tcaaatataa catgcctggt tcgagaataa ttagccatta
      541 tccaatacgt ttttttctta ttatgaaaac tgccaaatgc atcgtatgac gttcccttga
      601 cgcttcgatc accttctatt acgttgccaa tttgcttatc aacggttaat ttttgaatat
      661 gcaaaccaaa attcaactgc ggaataacat ttctggaaac cagaacattg gtgatagatc
      721 gtccttttcc ccctagcatt aaggaaaatt caatataagg tgatttggta tcgaagtatt
      781 taaagtcctt cggagctttg aaatagttgt cgaatccgcg gaatcccggc ctggcgccga
      841 tatctccttc gggttgatgg taaaccggtc tcatagcggt acctattact cccaggtcag
      901 tccacttcaa atctctcttc tctaaaaagg taaatttgtg gaaatcggtt agcaaagtat
      961 ctactgtttt ccagttatcg cgctgataga aaacatcctc ttctctaatt gataatgttg
     1021 atttaggtcc atatacaagc tttgttgagt catcaataat ctgcgatctt acctgagcgg
     1081 gcaactcaag actgaccaca attaatccgg aaaggtagag tagtttttta aacacgtttg
     1141 aataacgttt cgttttcagg taaattcatt tcttttaaca gcatcaagaa taattttgtc
     1201 aaatttcctg ccattcccaa caaaatcaat ctcgataggc aggtagaata aaggcactcc
     1261 ttcaagcaat gaatagaatt ttgatgaaat cagttttgcc ctgtcttttt ccgttgtgag
     1321 taaagaaata ttagatttac cgtctagcat acttatcagg ttttcaagat ccttgaaagt
     1381 atagatgtga tgatcagaaa aaacaacata atccagcaca tcgaacttgg aggttacaaa
     1441 taaacgaagc ggatccgggt tagcaagtcc gctaaaaata atgacaccag gagtaatttt
     1501 accttcgtaa gaacttaaca ccggcttggg atccagatac ctgataaacg agaagtatac
     1561 gggcttcgca ttagcatatt tttcgatttt tttcacatag ctatccatgt cgatctgttc
     1621 caactcatcc ggacatttgg tcactacgat cacatccgct ctcctggccc ctgttcttga
     1681 ttcacgcaaa ttaccggttg gcaatataag gtctttaaaa aatggtttgt catactcagt
     1741 aagaagaatt gataagtcgg gaattattcc cctttgctga aatccatcgt caagtattac
     1801 tatctccgta tctggaaatt caaacaagat atttggaatc gcgtaaactc tgtcttcact
     1861 aactacaatt ttaatttcat cactatattt ctgaaaaaac tgaaacggct catcacctat
     1921 agtctttgca tcatcattct tattagcaat tctaaaccca tagctttttc tcttgtatcc
     1981 cctgcttaag acagtgatct taaaattagt tttcaaactc ttgattaggt attctaccat
     2041 cggagtttta ccggttccgc ccattgataa attgccaaca ttgattacca tggtctcaaa
     2101 ctggaatgaa cgagtgtaat caatatcata aaggtaattg cgaagccggg taaccaaatc
     2161 ataaattaac gtgaccggga aaagcaaaag ccgaataaac cttattaggt actgcaagat
     2221 gaatttaata aatttgattc ctgcaaattt caaggcttaa tggtgaaaat aaaagaggtt
     2281 tcagattatt tagaaagtat cgcacctcga tcgctgcagg aatcttatga taactccggc
     2341 ttgctggttg gtaacccaaa aaccaatgtt tcaggcatat tggttagtct cgattgtacc
     2401 gaggaggtca tcaaagaggc aaagaagcgg aaatgtaacc tggttgtgtg tcaccatccg
     2461 gtcatcttta agggaataaa gagcataaca gggaggaatt acgttgaaag gactgtcatg
     2521 agcgctatca agtcagatat tgcaatatat gctatccata ccaatctgga taatgtgagt
     2581 accggagtta ataagaaaat cagtgataaa attggtctta taaacactca gattctagct
     2641 ccaaaaaatg ataatctttc taaattagaa acttttgtgc ctcctaaatg gaggaagaag
     2701 gttcttcagg caatccataa tgcgggagca ggtcgaattg ggaattactc agagtgcagt
     2761 tttattttgt cgggcagcgg aacctttctg cctaatgaaa aagcgaagcc caaactgggt
     2821 aaaaagaacc aattggaaac cgttgaagaa gagcgaattg aagttatttt tccaacagaa
     2881 ctcaaacgtt caataattag tgctttacag tatgctcacc cctatgagga agtggcctat
     2941 tatcttcatg acctccaaaa ctggaatgag gatactggat cagggatgat cggagacttg
     3001 ccagtgccag taagcagtgg ggcgttttta cttcagttaa agaagaaatt tggcttgaaa
     3061 attctgcggc atacagcttt cattaagtca aaagtgcgga gaattgctgt ctgtggcggg
     3121 gcaggcagtt tcttgatttc cagggcaaaa gccgtcaaag cggatgtttt tgttactgct
     3181 gacatcaagt atcatgagtt ttttgatgca gagggtgaaa ttttgatagt agatatcggt
     3241 cattatgaaa gtgaagtctt tacaaaagat ctcctttgca cgttattaag gaaaaaattt
     3301 tctaattttg cagtcaattt atcaaaaacg aacacaaatc cggtaaacta cttataagga
     3361 tacttcatgg aaaataatgt tgctaagaag ctggacgcct tgacccatct ccaggacatt
     3421 gactcaaaat tagattctat cataaaagtc cgaggggctc tccccgacga agtgcgcgat
     3481 cttgaggatg agattgcagg atacgaaacc agattggaga agataaacca ggaaattaaa
     3541 gaacgtgaaa ctgatatttc agataagaaa ttggccatca aggaatgcca gaagttgatt
     3601 aaaaagtatc aggatcaaca aatgaatgtt cgaaataacc gggaatacga cgcaatcacc
     3661 aaggagatgg aactgcagga gctggagatc caaataagtg aaaagaggat taaggaagcc
     3721 cacgcaaaaa ttgaagcaaa gaatgtggac attgccgagg tgaccgaaat aatgaatgag
     3781 cgaaacaaag accttaagag caaaaaaaat gaactttcct ctctcacttc tgaaagtgaa
     3841 gaagaagaaa agaaattatt aaaggaaaaa gaaaaagcag agaaatcagt agaagaacgt
     3901 ctgttgcttt cgtacaagaa gatcgtggct aatgccagaa acggtctcgg cgtggttagt
     3961 gtgaaaagag gagcttgtgg gggctgcttt aacgtagtgc cacctcagcg ccaggcggac
     4021 atcaaagaga ggaaaaaaat aattgtctgt gaacactgcg gaagaatact tgctgatgtg
     4081 gaaggtattg ccggtgaaga tgaaaagaaa ccggtcagaa gaagaaagta aaacttgtta
     4141 ttaccccgcg aacttcagat tcgcttgtta ctttttaagg tttcattata cgtttcaatt
     4201 ctaaacatgc tgccagagaa tagcaattgg tagagagaga agtttgtatg ttcaaaaatc
     4261 tccatcctgc tgagtggata gtttagcatt atacaaagta aagcccgaat agctcttcca
     4321 tgcatacaga ttaaaacctg tcgctcatca tttttggaca ttatatataa caaggccttc
     4381 gacagcctga atttcaaatc acttgggctt tcacccccag gcaaaggata atccaacatc
     4441 ccggatttcc atttaccaac aagttcccgg taatatttat catttttacc tccgaatttc
     4501 ttcccttcac tttctcccca atgtatctca ttcaggccgg ccaatttttc atgaggcact
     4561 ccggaatcta tgaacaattc catggactga acacttcttt gcaaggcaga agtatatact
     4621 ttatcgaagg agaaatcttt gtatttctca aaaaataacc gcgcctgatt atatcctgtc
     4681 tcatttagag aagagtcaat cccactcccc tggacaatac cctgcctgtt ctgttcagtc
     4741 tctccatgcc tcaccaggta tatttttttg atactcaatt ttcagttatt tttgtccagc
     4801 caaaattaac ccatttccac cagaatgttt aaagaagagc ttacagttga tttccttaat
     4861 aacttctgta aaaatactat ggttgacaat ttgggaattg aattcacaga aatcggtgat
     4921 gattatatga ttgccaggat gccggttgac aaccgaacta ttcaaccatt ggggttgctc
     4981 catggtggag cctctattgc tttggcagaa accctgggga gtgttgccgc tacctttatc
     5041 ctgaatacgg aaactcatta ttgtgtagga ttagaaataa atggaaatca tattaaagcc
     5101 attaaaagcg gctacgtttt tggaaaaacc accccgattc atatcggaag caagacacag
     5161 gtttggcaaa taagaattac taatgaaaaa gaacaactgg taagcatttg cagaattact
     5221 cttgcagtaa tagagaaaag aaaatagtat gaccgaaaaa gaaaaggcta ccttcatcag
     5281 ttctacctta tcttcccttg ataagaagga acttttgcac cgattgcttg gtaagtccat
     5341 atcggataat ttttctattg ccatttggcg aatgcccaat cagaattctt cacaattatt
     5401 tattgacttc aagcaaaata gggaatttga aatcgaagag ttagaagatt taccagccgg
     5461 atttgtattt gctccctttc ttgaaaatga taaaaagatt tacatccagg gagagctgca
     5521 aattgcattt agtgattcgt ctataaaatt aaattcatta gcccttactg aagataatct
     5581 gacctggctt gaagaagcct tgcaatataa gtctgatatc cggattaaaa ataagtttaa
     5641 tagtaaatct actagtaaat caaaatacaa aagctgggtt aatgaaggag taagtagaat
     5701 tatgaacggt gaattcaaga aagttgtacc ggctagaatt ttggttaagg aatggagggg
     5761 aaatccaatt gaaattttca atgatttatc ctgcaggtat cccaacgctt ttgtttccat
     5821 tacctgcaca cctcaggcag gatgctggat tggtgcaacg cctgaattat tattaagtgt
     5881 ggataagaat aagatattta gaaccgcttc actagccggt accaaggctt tcgatcagtc
     5941 agtgatgttg cataatgtta cctggactca aaaagaaatt gaagaacagg ctatggttag
     6001 ccggtatata attaactgct tcaagacaat tcgtcttaga gagtttgatg aggaaggtcc
     6061 aatgacaata aaagcaggaa acctggtaca cctaaaaacc aatttcattg ttgatatgaa
     6121 gagcacaaac tttccgaacc tgggatctac aatgttaaga ctactccacc ccaccagcgc
     6181 cgtttgcgga atgccaaaag atgcggctgc tgattttctg gagaaaatag aaaacttcga
     6241 tcgcacatat ttcagcggat acctgggccc gataaatgtt gaagatgaga caaacatttt
     6301 tgtaaatatc agatgtgcac gccttataga aaaccattcc ataatttttg ccggtgccgg
     6361 cgttaccagc tactctaatg ctgaatcaga atgggaagaa actgaattga aatgcaaaac
     6421 gctgctggac gtaatgtcat aatatcaaat cctatgcatt tacagccaat cagtaacata
     6481 gctgaaatat tttaccgcaa aaacatcacg catgtagtta tatcacccgg atccagaaac
     6541 gcaccactta cgcttgcttt tgccagacat ccggaaatag aagtgcatgt aattcctgac
     6601 gaaagatcgg caggatttat agcacttggc atttcacaaa acctaaaaca gcccgtgggt
     6661 gtggtttgta cttcaggcag tgcggcaatc aatctatttc cagcaattgc cgaagcatat
     6721 ctccaggaag ctccgcttat tattatgacc gcagaccggc ctccggaatg gatcgatcaa
     6781 tgggatggac aaactattta tcaacaaggg ttgtatggta tacatgtaaa acgcagttac
     6841 acactgcccg ttgatgtgtt gaataaggaa tctgaaacct tgttataccg gcaaatttca
     6901 gaaggtttaa attttgccgg agcactgcca aatggtccgg ttcatttcaa ttttcccttc
     6961 agggagccat tttatccgga caagaatgaa aaaattgaat attcaaaagg tcttaaaatc
     7021 attgattcag agaatccaga gtgctctata agcacatcga aacttgaaca tttatcatcg
     7081 ctgtggaaga gttcagcaaa aaaattgatt ttagttggtc agaatgatca ttctgaaaat
     7141 ctcttgtcgg tccttcagtt tgttgctgcc aagcacaaaa taccattggt aggagatgtt
     7201 ctctcaaata ttcatcatat agaaaattca atcaggtatt ctgacatctt tctctcaggc
     7261 aataaggaaa aacaaagtag cctgactccg gatttgctga taactattgg aaaatccgtg
     7321 atttctaaaa atctaaaact atttctcaga acttcccaaa tcaaaaacca ttggcaagtg
     7381 caatggggac aaaccattaa ggatccattt aattcaatca cagaaataat tattgtcgat
     7441 cctgttttgt tcctgaaaga aatggaactt ctatccggta gagacgattt tataaagcaa
     7501 aaacaaagca attttcaaga gttatggcat ttagaagaac ataatgctaa aaagtttttg
     7561 aaatcgtttt ttccgcagaa aaagtttggt gaatgggagg cgacaaaaga aattcttgga
     7621 tccttgccac aaaattcaat cctgcatttg gcaaacagta tgcctgtccg gtgggcaaat
     7681 ttttcaggat tacagcattt ccagagaata gatgtttatt gcaaccgcgg aacatccggt
     7741 atagatggct gcaccagtac aacaatgggt tttgccatta cctcacaaaa acctgttgtt
     7801 cttattaccg gagacatggc ttttctctat gatagaaatg ctttttggca taattacccc
     7861 tatcctttgc tgcgaattgt ggttctcaac aatcatggtg gaggtatttt cagattaata
     7921 acaggacccg atgaccaacc tgaactggag gagttttttg aaacaaaaca agttttaaag
     7981 gctgaatcag ttgctaaaga atttaacatt gactaccact atgccggttc agcggaacaa
     8041 ttagcaatgg tttggaaaga gttttttaat tttgatggta agccgaagct tcttgaaata
     8101 gaaaccaagg gagtcaccaa ccaggaaata tttagaaaat tcaaagaaga aactgaggaa
     8161 ttataacaat ggagatgaaa tacgattgga agccaattaa gccgtacaag gaaatacttt
     8221 ttcaatccta taatggtatt gccagaataa gtataaaccg tccggacgtt cacaatgctt
     8281 ttactccgct cacagtaaat gaaatgatcg atgccataac catttgccgg gaaagccccg
     8341 acctgaaagt tgtgatcctt accggtgaag gcggccagtc attctgtagc ggtggtgacc
     8401 aaagcgttcg tggacatggt ggctatgttg gcggtgatca ggtgccgaga cttaatgttc
     8461 ttgacttgca aaagcttatc cgttcaattc ctaaagcagt aattgccgcg gtagcaggtt
     8521 gggcaatcgg cggtggacat gtgcttcatg ttgtttgcga cctcactatt gctgctgata
     8581 atgcaaaatt tggacaaacg ggtcccaaag ttggaagttt tgatggcgga tttggttctt
     8641 cctacctggc caggattgta ggtcaaaaaa aggcccggga aatgtggtat ttatgcgacc
     8701 aatatgatgc ggttgaggca gacaaaatgg gtcttgtaaa taaggttgtc cccctgaata
     8761 ggcttgaaga aacttatgtg gaatgggctg aaaaaattat ggagaaaagt cctattgcaa
     8821 taagaatgtt aaaaagcgcc ttcaacgcgg aacttgacgg ccaggcaggt attcaggaac
     8881 ttgccggtaa tgccactctc ttgtactaca tgtctgacga agcgaaggag ggcaaaaatg
     8941 ccttcctgga aaaacgaaag cccgacttcg ataaatttcc aaaatttccc taatctgatt
     9001 tgaataaaat tttctataga ggtaagaagt acgatctatc caaaacggga gaaagagaag
     9061 atttagtaag actgagtgaa tttagcagga atgtcactga acttgccacc aaatggttgt
     9121 caggcggtaa agaattttca tttcaaactt caggttcaac aggggagttc aaagaaatca
     9181 gtcattcgag agaaaaaatt caaaaatccg cgaagttgac catcagctac ttcaatctaa
     9241 ataaagatga taatgccctg ttggttcttg atccaacccg aattgcagga gcgatgatga
     9301 tcatccggtc attggtggca ggaatggatt tatatgccat tgatcctact tctgatctta
     9361 cctccttctc acctatattc agagattgca attttgctgc catcgttccg ctgcagcttg
     9421 agaaactatt attaagcaaa gaggtctcag aaattttcag aaattataaa gcagttttgg
     9481 taggtggtgc cacagtcaat tatgctatga tagaaaagtt gaaaggaatg gccttgccat
     9541 tttatgaaac ttttggaatg acagaaactg tgacacattt cgcattgcga aaattatccg
     9601 gttcagatac taatccgttt gaagttatag ggaatcatat tgtgaggcaa gattcgcacg
     9661 ggtgcatgat agtggaagga gatttaaccg ttaatcagcc tctttcaacc catgatatta
     9721 ttcaaatcag ggatgaaaga cattttgaat gggttggaag gagtgatttt ataataaaca
     9781 ccggaggtat aaaaataagt cctgaggaat tggagcgaaa aattgcgggt aaaatttatg
     9841 cttttggaat ccggaaccgg tttattatca gttccgtcca tgatgaatca ttgggtgaaa
     9901 aaattatatt ggtgtttgaa ggaaccacaa tttctgatca tataatttct gaactaatga
     9961 aaaccattaa agaggatctg gataagtacg aatgcccaaa agagattcga tatatagaga
    10021 aatttcctga agcagccatc tccaaaataa accggagggc agttatttcg attttagctt
    10081 agctacctca tctttaagtt ccataacaat agatgccagt gattctatgg aaacattttg
    10141 attgtcattt gggtcaggaa attttacact tatataagcc ttcgcttccc agatctctaa
    10201 cacatcttcg gccggaatat catatgagga gtaactttta ttatcagaaa ccagaaaaag
    10261 ttttccttta tctttcacat aattaaatat acgtttgaaa accactcctt ccttacccgt
    10321 cacaagaaca taagtttttc catcttttat agaatccagg gtttccacat actgaccaac
    10381 tactactgtt cccgattcaa gggggagcat tgaatctcct gaaatctcaa aggcacgata
    10441 ggaagcattt tgaggaagca tgggaagctg aaaccgcgga agttcactaa tgtattccgg
    10501 atctgcatat ccatttagat agccggctgc agctttttga ggaacgagat gaatattttc
    10561 cttattttct ttgtctaagg ttatggataa gatccgcagc ggtgccttat cagttttcct
    10621 gtaagtaaat ttttcatcag taacagtcag gtccgatgtt attaagctat caattgcaat
    10681 tctaaaggcg ttggcaattt tagtgagatt ttcgatccgg ggatcagctc ttccttcttc
    10741 atacgctcca attaacgatc ttttgattcc cagttccgag gccaaatcat cctgtgtcat
    10801 tccatttttc ttgcgaagaa atttaatatt ctcactgatt agcgacataa gtttgatttt
    10861 tatctaaggt ataatttact tttcaaagat catgatatca tcttccctgt tccttcgcac
    10921 tgccatggaa tataaatgac catgataata ttcatccctt tcctggatag cattgatctt
    10981 ttgctgaatc ttgctcagga catcattgaa tgttgcctcc ggttctgcca gcagatagcc
    11041 ataccatcgc ttttgcgcgg cattttttac ctcaaatttt acctggtggc gccctgagtg
    11101 aagttttttg ctgcacaatt tcaatcggaa attatcatca gcgttaacgg atatcatatt
    11161 catctttaaa aatttaccac ttatattagt atgtatgctc ataaaattag tattattgtt
    11221 gaataaaaca aaatttatac taattatttt agtagatgct tttttctcac cccgatcgcg
    11281 ctataatcca catggatttg gacacattct tcgtgtcggt ggagcggctt aggaatagtg
    11341 agttaaatgg taaaccttta gtaattggcg gccatagccg gcgcggggtc gtagcttctt
    11401 gcagttacga agcccgcgca tttggcattc attccgccat gcccattggg ctggctaaac
    11461 agctgtgtcc ggatgtactt atcatttcag gcgatctgga agcgtacagc aaatattctt
    11521 ctatcatcac cgaaattatc cgcgaagagt cccccctttt tgagaaagcg tctattgacg
    11581 aattctatat tgacatcacc ggaatggata ctttttataa ctcgtatcag tgggggaccg
    11641 agctgcggca aaaaataatc cgtgaaaccg gactgcccat ttccatgggg ctttcggtaa
    11701 acaaactggt atctaaggtg gccactaatg agtgcaaacc caacggacaa agccatattc
    11761 aaaaaggcct ggagcgggaa tttctggcgc cgctcgctat ccagaaaatt cccatgattg
    11821 gtgataagac cacccaattt ttaacgcaca tgggaatccg aaccgtacgt actttgcgtg
    11881 aaatgccgtt aaaaatttta caaagcacct tcggcaaaat tggtatcagc ttatggaaaa
    11941 aagctaatgc tatggatgat gcccctgttg ttgcttatag cgatcgtaaa tctatttcca
    12001 cggaatgtac ttttgaatcc gataccatcg atgtaaaaaa attaaaaacc attattatgg
    12061 cgatggtgga aaaactggcc tataaactgc gtgaagaaaa aaagctaacc gcctgtatag
    12121 ccgtaaaaat acgatattca aattttgata cggtttcgaa gcaacgtcat ctccctttta
    12181 cttcttcaga ccaggccttg attcgtgttg ccatggagtt atttgatcag ttatacagca
    12241 gacgcctgtt gattcgactg gtgggtgtgc ggctcagtaa cctcgtgtat ggtaattacc
    12301 agattaacct gtttgatgat acggaagaag aaatccgttt gtaccaggct atggacaggc
    12361 ttaaattaaa atacgggccc gatactattt tccgtgccgc cacacagaat gtgaatcaca
    12421 ggattcggct ggattcgaat ttatttaaag gatgaaaaaa gttgtaagtc gtaagttgtg
    12481 agtcgtaagt cgtaagttgt aagttgtaag ttgtaagtcg taagtcgtaa gttgtaagtc
    12541 gtaagtcgta agttgtaagt tataagttgt aagtcgtaag tcgtttgtat ctgaattgcc
    12601 attcctggtt tagtttcaaa tatggaactc tttctccgga agatttgttc gaagaagctc
    12661 agcgctgcgg aataaaaaaa cttgccctca cagatatcaa taatacttgc ggatacattg
    12721 agatgttacg tatttgtaat gagaaaagaa aaatttataa tctggaaatt atacctggca
    12781 tcgaattcag aagagataat caactcattt atatagccat tccttatgat aatgccgggt
    12841 ttgaagaaat aaaccgcttt ctttcctatc ataataatac agaaaagccc ctgccccatc
    12901 atgcaccgga gttcagcaac gcgtttatta ttttcccctg gaaaaatgca ccggaatttt
    12961 taagagaaaa tgaattcatt ggcattcagc ctcatgaaat taataaaatg gttagctctt
    13021 ctttggaaaa aatgcttcat aagctggtgg ccttgtatcc cgttaccgtt aaaaataaag
    13081 ccggttataa cacgcaccgg ctgctaaggg cagtcgatca gaatgtggtc ttaagcaaac
    13141 tatccccgga attgcaggga agaccagacg aaatcatgat caatgagtca gaactgctgc
    13201 ttcgttatgc caggtatccg gaactggtgc ggcgcagcag cgaaattctg aatcaatgtt
    13261 ctttctcgct tgaattttat acagataaaa acaaaaagac ttttactgaa agtaaagagt
    13321 cagaccgttc tttacttgag tcccttgctt tggaagggtt atcaaaaagg tataatagtg
    13381 gatttaagcc aaaagccagg ctttatcatg agctggagat tatctctcga cgcagttttg
    13441 aatcgtattt tctgattgcg cacgacatca tcagctttgc aaagcacaaa aattttgctt
    13501 ttgtagggcg aggcagcgga gctaacagca ttaccgccta ttgtctgggc ctgacccagg
    13561 tcgatccttt ggaactggat ctctattttg aacgatttct gaatcctgag cgtagttcac
    13621 cccctgattt tgatattgat ttttcctgga aagaccgtga tgaagtaaca acctacatct
    13681 ttaatcgcta tcataaggat cacaccgcat tgcttggtac acacactaca tttaaagacc
    13741 gttctataat ccgtgaattg ggaaaagttt ttggcttacc caaaccggag atagacaatt
    13801 tggtcactta ccccgacacc taccggcaca aagacaacat cacagaaatg atttttaaat
    13861 atggagaaag aatgctctcc attcccaaca atctttccat tcatgcaggc ggtgttctca
    13921 tcacagaaaa accgatttac gcttatactg ccaccgactt gccacccaaa gggtatccta
    13981 ccagtcattt cgaaatgcac aatgcagaag atatcggcat atacaaactt gatattctca
    14041 gtcagcgtgg tttgggtcac atcaaggata ctatagctac tgtaaaatcc aaccaaggca
    14101 ttgatattga tatcaaccgg tttcaggatt tcaagaaaga cgctgacatt aaaaaactgt
    14161 taagcaaggg gcatactatg ggttgtttct acgtagaatc tcctgccatg cggcagctgc
    14221 tggggaaact ggagtgtcag gattacataa cccttgttgc ggccagttcc atcatacgac
    14281 ccggtgttgc ccgttccgga atgatgcggg cgtatattta ccgccatcat catccaaatg
    14341 attatcagtc aatacatccg aaaatggatg agctgatgaa agaaacttat ggagtgatgg
    14401 tataccagga ggatgtgata aaagtagcgc atcatttcgg cgggttaacc ctttccgaag
    14461 ctgatatttt acggcgggga atgtcaggta aattccgatc cagagaagaa tttaaacgtg
    14521 tggaagtaaa gtttttcagc aattgcaaag agcgcggcta tgcagatgag gtcaccgcga
    14581 gagtatggta cgagattgaa agtttttcgg ggtattcatt ttccaaaggg cattcggcaa
    14641 gctatgcggt agagagttat cagagtttat ttttgaaagc tcactacccc ctcgaattta
    14701 tggttggcgt gattaataat tttggggggt tttacagaac tgaattttat tttcatgaag
    14761 cccgtatgaa cggtgccacc atcgaagcac cctgtgtaaa taacagttgt taccttactt
    14821 ccatcacagg tacttgtatt tggatgggat ttatccattt gaagagtctg gaaattaagg
    14881 ttgccggaca gattgaagag gaaagaaaca aaaaggggaa attccgaagc cttgataatt
    14941 ttattcgtcg cattcctatt ggtcttgaac aagtgtccat cctgatccgg ataggagcat
    15001 ttcgctttac cggaaaaacc aagcgtgaac tactttggga agcgcattta tattttggca
    15061 aagtaaacaa aaatgaattt gccgaagcca gtaatgagtt atttgaaaca gagcctatgc
    15121 aatacaaact tccacaactg gagcctgctc cattcgaaga tgcgtttgat gagcttgagt
    15181 tattgggctt tccgctttac cacccttttg atctacttga aagtaaacca cgtgcagaag
    15241 ccaaagctga agatctaaag aaaaaaacaa atcagaagtt ggaaataatc ggctatctga
    15301 tcacaattaa gtatacatca accaaaaata accttcccat gcagtttggg actttctatg
    15361 atgaagatgg agaggttttc gatactactc attttcccgg atcggcgagg aaattccctt
    15421 tccgcggaaa aggattttac agaatcaaag gaaaagtggt tgaagatttc ggttatccaa
    15481 tgattgaagt c
//
LOCUS       DASWHX010000110         7169 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_1201333, whole genome shotgun sequence.
ACCESSION   DASWHX010000110 DASWHX010000000
VERSION     DASWHX010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 7169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7169
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1201333"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1631)
                     /locus_tag="VGA21_07620"
     CDS             complement(<1..1631)
                     /locus_tag="VGA21_07620"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HEX9650412.1"
                     /translation="MKSSFLSLLLLFVSGYLIAQHNLETVFEKSNGLRTPTYLEGIEY
                     FKRLAGSSDFLNIREIGITDSGQPLHLVVFSHSKEFEFDKLRARNKYFMLVNNAIHAG
                     EPDGVDASMLFLRELTTNASLRNELQNVVIAVIPFYNIGGVLNRNSTTRINQLGPESY
                     GFRGNAQNLDLNRDFMKMDSRNARSFAEIFHLIKPDLFVDNHVSDGADFQHVVTLIAT
                     QHNKLGGEMGVYLHETLVPGLYKEMAERKYDLVPYVNIFNANPDRGFSEFKDGPRYSS
                     GYAALFQCFSFIIETHSLKPFKDRVLATYELNKAMAQYVAKNGKTMRELRLLDQKKFR
                     SSTQYFLDWQLNRSVSTKLFFKGYESAMVKSKITGNERLQFDRGKPYEKEIDFYDTYN
                     PSGTVEVPGFYVIPQGWTKVVERLQWNQIRMRRVQSDSSFEATVYLITNYEHARNPYE
                     GHYPNNSAELNEVTEDITVRGGDYIVPTDQPGLRYIMEVLEPQGPDSFFSWNFFDAIL
                     QQKEYFSPYIFEELAPGILNENPDLKNNFEERIKA"
     gene            1678..2262
                     /locus_tag="VGA21_07625"
     CDS             1678..2262
                     /locus_tag="VGA21_07625"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HEX9650413.1"
                     /translation="MNEKEINTIIRNRRSVYPKQYAGGDVSKETIMEILENANWAPNH
                     KRTQPWRFHVFMGKGLQRLANFQATLYWENSTKRGDYDEVKFEKLKITPLKAACVIAV
                     SMKRSDPNLIPEIEEIIATGCAIENIYLSLSFYGLGGYISTGGGTYDPQTKNFLGLEV
                     ADKLIGYFYIGTYDGPVEEKSRKPVAERITWIED"
     gene            complement(2259..2960)
                     /locus_tag="VGA21_07630"
     CDS             complement(2259..2960)
                     /locus_tag="VGA21_07630"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX9650414.1"
                     /translation="MNKFSNRSSEIEIMDDLDCSGEVVHRTLHEIEFINKWLGGNTIT
                     LHEVKSLLLKNKSPNEIRVADLGCGGGGMLKEISELCARLGVRARLTGIDANPNIINY
                     AKSGNADRPQIEFLCLDIFSEEFRLMQFDIILCTLVAHHFSNKQLSELFKNLKNQATL
                     GVVINDLERNPLAFYSIRFLTSVFSKSAMVKFDAPLSVLRGFKKTELQSILQKAEIDT
                     YNLSWQWAFRWKLVF"
     gene            3445..4536
                     /locus_tag="VGA21_07635"
     CDS             3445..4536
                     /locus_tag="VGA21_07635"
                     /inference="COORDINATES: protein motif:HMM:NF013779.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C10 family peptidase"
                     /protein_id="HEX9650415.1"
                     /translation="MITIESAERIAGHLLFGNFDGKSPKEDLKSVRKSKKEVESILEV
                     PDENNIAAYYIINYKEGGFVILAGDKRLEPVLAFSETNRFDLDTEYFPSGLVGWLYSL
                     KENIEAIRIKNDKIEPEAEILWNTLLSAETDVVTYLIKPEEDTDCTPYTIQKGPFLQT
                     TWGQGCGYNSQTPSMSCGECGHAWTGCVATAMAQVMRYHQYPASYSWSNMPNSSGTAT
                     TAGLMSDIGNAVNMSYSCDGSGADTKSKIVSAFKNTFGYSTASYADYNYQTVENELRY
                     NRPVILRGGRNTGWWIFGQYSDGHAWVCDGYLSYIDPCWGSILKFHMNWGWGDNATNN
                     YNGWYSFNNFNPSNYTFNYKTGMVYNIKP"
     gene            4548..5012
                     /locus_tag="VGA21_07640"
     CDS             4548..5012
                     /locus_tag="VGA21_07640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650416.1"
                     /translation="MRHLIISMAMVLFSSCISEDDQFIVDVGIGISVKGSIGEDLLDP
                     ENPSSYKESDIKLHYLKNGQKQEVFFPNLDNPKGFGITQNQEGYRIGILPNIDKTEEF
                     PLTLIEWNSTDTDTIKCSIERKSNSEICTKVWLNENLVWEGYETERFFEIIK"
     gene            complement(5220..5681)
                     /locus_tag="VGA21_07645"
     CDS             complement(5220..5681)
                     /locus_tag="VGA21_07645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650417.1"
                     /translation="MKAIFGFLALISAMFIGCEDDRFTPYIANIDDCHFSLDLDSVET
                     VNRLIGTWKWVYEDQLTLNGYVGTTTSHLGLSIQIKSNGELVIYNNEVVEVTTWTIKP
                     EAGGSAWILNTHPFHGYTYGPLRFCDDYFAVRNSLTDASDDYYQKVDETAG"
     gene            5910..>7169
                     /locus_tag="VGA21_07650"
     CDS             5910..>7169
                     /locus_tag="VGA21_07650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650418.1"
                     /translation="MAQQTPVQVSAFLNPPYSLALYEYGMPGSNLLQVQLLLTDLTKN
                     QYPVKLRLIVEGVGITIKTKSTFLPQPIPLSGGTPYILTAEDLQPYFSLENLDLLGIS
                     KRDYQRLGMLPEGFYRFTIQVLDYYRSSVVSNSAMAQAWIILADPPFLNYPMKEEKLI
                     ARDPQNIQFSWSPRQQSPNLGLTEYTLQLFEILPEGSNPYNTAASTQPYFETTTYNTA
                     YFYTLSDPMLVPGRQYAWRVKAANTEGYDRYKNDGYSEVRAFRWGDACEEMLNVTVTP
                     FSTFVKITWEPQFGQTQFKIRWHSAKPGATVWTETTNLDFFIIKDLVPGETIAVSVAG
                     MCGSITGLYTPEFEVTTLYVEQEVTDFECGKPTIMETLTGSPPLQQVYPGMRFTNVGG
                     WELEVIEVESSENGVFNGTCWWTIPMFK"
BASE COUNT         2233 a         1441 c         1456 g         2039 t
ORIGIN      
        1 tcagccttaa ttctttcctc aaaattgttc ttcaaatcag gattttcatt cagaattccc
       61 ggggcaagtt cttcaaaaat ataaggggaa aaatattctt tttgttgaag aatagcatca
      121 aaaaagttcc aggaaaaaaa agaatccgga ccttgcggtt ccagaacctc catgatatac
      181 ctgagcccag gctggtcagt tggcacaata taatccccgc cgcgaacggt aatatcctct
      241 gtaacttcat ttaattccgc ggagttgttg ggataatgtc cttcataggg attgcgtgca
      301 tgttcataat ttgttatcag gtaaacggtt gcttcaaatg aggaatcact ttgcactctt
      361 cgcattcgaa tctggttcca ttgcagtcgc tcaacaactt tcgtccatcc ctggggaata
      421 acatagaaac cgggtacttc aaccgttccg gatggattgt aagtatcgta aaaatcaatc
      481 tctttctcat aaggtttacc gcggtcaaat tgcaatcttt cgttaccggt tattttgctt
      541 ttcaccattg cggattcata gcctttaaag aataatttgg tcgaaacact tctgtttaac
      601 tgccaatcaa ggaaatattg agtagaacta cgaaacttct tttgatccag gagccttagc
      661 tcacgcatag ttttaccatt ttttgccaca tattgagcca tcgctttgtt taattcgtaa
      721 gtggccagaa cacgatcttt gaatggcttg agactgtgag tttcaataat aaagctgaag
      781 cattgaaaaa gcgccgcgta cccgcttgaa taccgcgggc catctttaaa ttccgaaaag
      841 ccccggtcag gatttgcgtt gaaaatattt acatagggaa ccaagtcata ttttctttct
      901 gccatttcct tatataaccc gggaaccagg gtttcatgaa gataaactcc catctctcct
      961 cctaatttgt tatgctgagt tgcaattagc gttactacat gttgaaaatc tgccccatcc
     1021 gacacatgat tgtcaacaaa taaatcgggt tttataagat ggaatatttc cgcaaatgaa
     1081 cgggcatttc gtgaatccat cttcatgaag tcacggttca gatcaagatt ctgcgcattg
     1141 ccccgaaagc cataggattc gggacccaat tgattaattc gagtcgtaga attacggttt
     1201 agcacaccgc cgatattata aaagggaatt acagcaatta caacattttg taattcgttt
     1261 ctgagggatg catttgttgt aagctctcgc aggaaaagca tcgaagcatc aactccatcc
     1321 ggttctcccg catgaatagc attatttacc agcataaaat atttattccg ggcacggagt
     1381 ttatcaaatt caaattcttt cgaatgcgaa aagacaacca ggtgcagtgg ttgtcccgaa
     1441 tcagttattc caatttccct tatatttaaa aaatctgatg agcctgccag ccttttaaaa
     1501 tattcaattc cctcaaggta agtaggagtt cttaatccat ttgatttttc aaaaacagtt
     1561 tccagattgt gctgggctat cagatatccc gaaacaaaca ataacaatag tgaaaggaat
     1621 gagctcttca taacttaagt taactttgtt gaaccaaatt ccaatttagc ataaactatg
     1681 aatgaaaagg aaataaatac gatcataaga aacagaagat cggtatatcc caaacaatat
     1741 gcagggggag atgtgtccaa agaaacaatt atggaaattc tggagaatgc caattgggcg
     1801 ccaaaccata aacgaactca accctggcgt tttcatgttt tcatggggaa gggtcttcag
     1861 agattggcca attttcaggc aactctttat tgggagaatt caacaaaaag aggagattat
     1921 gatgaagtta aatttgaaaa actaaagata actccattga aagcagcatg cgtaatcgcg
     1981 gtctctatga aaagaagtga tcccaatctc attcctgaaa ttgaagaaat tattgccaca
     2041 ggttgtgcca tcgaaaacat ttacctgtca ctctcttttt atggtttggg cgggtatata
     2101 agcacaggag gcggaactta tgatccacaa accaaaaact ttttaggtct tgaagtagcg
     2161 gataaactaa tagggtattt ttacatcggc acttatgatg gcccggtcga agaaaaaagc
     2221 cggaaacctg ttgcggaaag aattacctgg attgaagatt agaaaaccaa tttccaccgg
     2281 aatgcccatt gccaggagag gttatacgta tcaatttcag ctttctgtaa tatggattgt
     2341 aattctgttt ttttgaaacc ccgcagtacc gacaatggtg catcaaattt caccattgca
     2401 gacttggaaa aaaccgaagt cagaaacctg attgaataaa atgctaacgg gtttcgttcc
     2461 aggtcgttga tcacgacccc aagagttgcc tggtttttca aatttttaaa caattctgaa
     2521 agttgcttgt tggaaaaatg atgagcaacc agcgtacaca agattatatc gaattgcatc
     2581 aatcgaaatt cctccgaaaa aatatccagg cacagaaatt caatttgagg tctatccgcg
     2641 ttacctgatt ttgcatagtt aataatattt gggttagcat caatgcctgt caaccttgca
     2701 cgaactccca acctggcgca taattcactt atttctttta acattccacc gccgccgcac
     2761 cccagatcgg caacccggat ttcattcggt gatttgtttt tcagcaacag agacttaacc
     2821 tcatggaggg ttatcgtatt tccacccagc catttattga tgaattctat ttcatgcagc
     2881 gtcctgtgga caacctcgcc ggaacaatcc agatcgtcca ttatttcaat ttcagaagat
     2941 ctattactga atttgttcac tgaatcaatt ttgattcatg aaaatacttt aaaaatcaca
     3001 aaccctgaat tgtggactcc taagtcaccc ctgaatttca tttcagggtt ctgatttttc
     3061 cctttagatc gaattttcgt gtccgtaact tatagtcttt tccagtgaaa aatcagagat
     3121 atgtacagct attagcaaat tacatataaa ttatcaaata gtaatttgta ttttaaaaat
     3181 aagcttccta ccttcaaaac caataaaatc taacccgtaa gatttagcta aacctgagcc
     3241 gggcaaggaa tgctacgggg tcctccccat agcatgtcgt tgcgagtgat ttttataatt
     3301 agaccattaa aaagttttaa ctaaatccca aagactatga acaaatcaaa tcagattaaa
     3361 tggttattcg tgttcctgat aggaatacta ttattttcat gtgataatga agaagcacta
     3421 aaaattgatt attaggacga aaaattgata accatagagt ctgccgaacg cattgctgga
     3481 catttactat ttggcaattt tgacggcaaa tcaccaaaag aggatctaaa gtcagttagg
     3541 aagtcaaaga aagaggttga atccattctt gaggttcccg atgaaaataa tattgctgca
     3601 tactacataa tcaactacaa ggaaggaggt ttcgtaattt tggctggaga caagagattg
     3661 gagccagttt tagctttttc agaaaccaat cggtttgatt tagatactga atattttcct
     3721 tcaggtctgg ttggctggtt gtattcttta aaagagaaca ttgaagctat aagaatcaaa
     3781 aatgataaga tagaacctga agcggaaatt ttatggaata ctttactaag tgcagaaact
     3841 gacgtagtaa cctacttaat aaagcctgaa gaagatactg attgcacacc atacacaatc
     3901 caaaaagggc cgtttcttca aacaacttgg ggccagggat gtggctataa ctcacaaact
     3961 ccatcaatgt catgtggcga atgtggtcat gcttggacgg gttgcgtagc aacagcaatg
     4021 gcacaagtga tgagatatca ccaataccca gcaagttact catggtcaaa catgcccaat
     4081 tctagtggta cagctacaac agcaggacta atgagtgata taggaaatgc tgtaaatatg
     4141 agttatagtt gtgatggatc cggtgctgat acgaaaagta aaatcgtatc tgcatttaaa
     4201 aacacctttg gatacagtac agcatcttat gctgactata attatcagac cgtagaaaat
     4261 gaattgaggt ataaccgacc agttatatta cgaggtggta gaaacactgg ctggtggatt
     4321 tttggccaat actctgatgg ccacgcttgg gtctgtgatg gatacttaag ttatattgac
     4381 ccttgctggg gttctatttt aaaatttcac atgaattggg gatggggaga taatgcaact
     4441 aataattata acggatggta cagttttaac aattttaacc ctagcaatta tacgtttaat
     4501 tacaagactg gaatggttta taacatcaaa ccttgaattt taagactatg agacatttaa
     4561 ttatttcaat ggcgatggta ttgttttctt catgtatttc agaggatgac cagtttattg
     4621 tggatgtagg aattgggatt tcagtgaaag gttcgatcgg agaagattta ttagatccag
     4681 agaatccgag ctcctataaa gagagtgata ttaagcttca ttatctgaaa aatggacaaa
     4741 aacaagaggt ttttttccca aatttggata atccaaaagg ctttggtatt acacaaaatc
     4801 aagaagggta taggatagga attctaccga atatagataa aactgaagaa tttccattaa
     4861 ctcttataga atggaatagt acggatacag atacaataaa atgcagcata gaaaggaaaa
     4921 gcaattctga aatatgtaca aaagtttggt tgaatgaaaa cttagtttgg gaaggatatg
     4981 aaacggaaag gttttttgaa atcataaaat aaaggccaca agcaacacct ggacatcaat
     5041 ttatatggag gatgggatct gcagcctctt tcagtttgat acattgcaaa aaattaaata
     5101 ctggattgga gaactgaccg tatggtaaga aatcttgatt atcgagattt tcaaatttgg
     5161 gattcgagtt catgaaaggg acttggtaat gtgcagtttg taaattcaat catgacaaat
     5221 cagccagcag tttcgtcaac tttttgatag taatcgtcag aggcatccgt aaggctatta
     5281 cgtacggcga aataatcatc acaaaaacga agaggaccgt aggtgtaacc atggaatggg
     5341 tgggtgttca atatccaggc agaaccgccc gcttctggtt taatggtcca cgtggtaact
     5401 tcaaccacct cattattata gatcactaac tccccatttg attttatttg aattgataaa
     5461 ccaaggtgag aagtagttgt gcctacataa ccattaagag taagctgatc ctcataaacc
     5521 catttccagg tgccaatcaa tctattgacc gtttcaacag aatcgagatc taatgaaaaa
     5581 tgacagtcat caatgttagc tatgtatggg gtgaaacggt catcttcaca gcctataaac
     5641 atggcagaga tcaatgcaag aaatccaaat attgctttca cttattgaat cttgacaatt
     5701 tttcttgttg taataaagtc cttaccgaca accatgagac agtaaattcc aggtttatag
     5761 ttcttaacgt ctaattcaat aaatatttca tctttttgat tgcactattt agatttattt
     5821 taatatttat caaagtattt cttaattatt ttgtgataaa taggcttttt ctaatatttt
     5881 attatgtgtt tgtggctcca ttggctctaa tggcacaaca aacccccgtg caggtatcgg
     5941 ccttcctgaa tccgccctat tccctggctc tttatgaata tggaatgccg ggcagtaatc
     6001 tgctgcaggt acaactactg ctaactgatt taaccaaaaa ccagtacccg gtgaagctca
     6061 ggctgatagt ggagggagtg ggcatcacga taaaaaccaa atccaccttt ttgccccagc
     6121 ccattccact cagtggcggc acaccttata tattaaccgc cgaagatctg cagccctatt
     6181 tcagtttgga gaacctggac ttactgggca tcagcaaaag agattaccag cgactgggca
     6241 tgctgccgga aggcttctac cggttcacca tccaggtgct ggactactac cgctcctcgg
     6301 tagtgagtaa ttcagccatg gcccaggcct ggatcatcct ggccgatccc ccgtttttga
     6361 actaccccat gaaagaagag aaactgatag cccgggaccc gcagaacatc cagttcagct
     6421 ggagtccccg gcagcaatcc cccaacttgg gactaaccga atacaccctg caactctttg
     6481 agatattacc tgagggcagc aatccctaca acacagcggc atctacccag ccgtattttg
     6541 aaaccaccac ctacaacacc gcttatttct ataccctgag cgatccgatg ctggtaccgg
     6601 gccgtcagta tgcctggcgg gtgaaggccg ccaacaccga aggttatgac cgctacaaaa
     6661 acgatggcta cagtgaggta cgcgccttcc ggtggggcga tgcctgcgag gaaatgctga
     6721 atgtgaccgt tacgccgttt tccactttcg taaaaatcac ctgggagccg caatttgggc
     6781 agacccagtt taagatacgc tggcacagcg cgaaacccgg tgccacggtg tggacagaaa
     6841 ctaccaacct ggatttcttt atcatcaaag atttagtacc cggggaaacc atcgctgttt
     6901 cggtggcggg gatgtgcgga agcatcaccg ggctttatac gccggagttt gaagtaacaa
     6961 cgctttatgt tgaacaggaa gtaaccgact ttgaatgcgg gaaacccacc ataatggaaa
     7021 ccctgacggg gtcgccgccc cttcagcaag tttaccccgg catgcgattc accaacgtgg
     7081 ggggctggga gttggaagtg atagaggtgg aatcaagtga gaacggagta ttcaacggta
     7141 cctgctggtg gacaatcccc atgttcaaa
//
LOCUS       DASWHX010000111        31911 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_1962407, whole genome shotgun sequence.
ACCESSION   DASWHX010000111 DASWHX010000000
VERSION     DASWHX010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 31911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31911
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1962407"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(70..843)
                     /locus_tag="VGA21_07655"
     CDS             complement(70..843)
                     /locus_tag="VGA21_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017730009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX9650419.1"
                     /translation="MNQPSLFYREQGTGDPIIILHGILGSSDNWMTVARSLSSDYKVF
                     TLDQRNHGQSFHSDKFDYPSMVNDLKKFIAEHEIVNPFIIGHSMGGKVAMNFAISFPE
                     LLKELVIVDISPRSYPIHHDNILEGLSSIELDKLQSRNDADTQLSKYVSDSTTRNFLL
                     KNLSRDETGNFTWKINLPVIRMKMEWVGIGLPGNDKFEKKTLFIKGEKSNYINEQDYD
                     LIKARFPNSEIKVIKDAGHWVHAEQPELFITLLNDFLKS"
     gene            complement(840..1553)
                     /locus_tag="VGA21_07660"
     CDS             complement(840..1553)
                     /locus_tag="VGA21_07660"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013453919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HEX9650420.1"
                     /translation="MTRLSVNINKIATLRNARGGNNPDLLQIALDCERFGAQGITVHP
                     RPDERHIKYKDVMDLKDIVKTEFNIEGYPDERYLQIIETVRPAQATLVPDPPDVLTSN
                     AGWDTIKEKDFLFSTIRKLKGFGVRVSIFIEPDLQMIEAAAKAGTDRIELYTEAYAKG
                     YSSDRAEAIAPYIKAAELANELGLGINAGHDLDLDNLKFLKTSIPFIDEVSIGHALIC
                     DALYLGLDNAIKLYLKELQ"
     gene            1611..2060
                     /locus_tag="VGA21_07665"
     CDS             1611..2060
                     /locus_tag="VGA21_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010611730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEX9650421.1"
                     /translation="MSLKQRIDAEIKKAMLAKESDRLRALRGIKSLILLAESEKENRS
                     GLSEKVETQILTKAAKQRKDSAEIYNQQSRDDLAGKEIFELKVIEEFLPEQMSEKEIE
                     RQVSEIISETAAEGMKDMGKVMAIATKQMAGKADGRIISEIVKKKLS"
     gene            2062..2574
                     /locus_tag="VGA21_07670"
     CDS             2062..2574
                     /locus_tag="VGA21_07670"
                     /inference="COORDINATES: protein motif:HMM:NF014704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEX9650422.1"
                     /translation="MNSLDWIILAFLTFAAFQGYRKGFILEIISVLALILGIIGGLKL
                     IDFGKEFLSRHLEIKGDILPYLSFLFIFIIIVILLNLAGRAVKKILDMTLLGNLDDIA
                     GLVIGILKWTLILSSLIWIASIIDLKLPFTWTEDSILYPYVSWLAPNLFHMLSGVFPF
                     LEELIAYFAS"
     gene            2661..3413
                     /locus_tag="VGA21_07675"
     CDS             2661..3413
                     /locus_tag="VGA21_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019988127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9650423.1"
                     /translation="MGKGFNLKVDNGFTYIEEGEGEVILLLHGLFGALSNWKWVLEHF
                     SSNYKVVIPMLPIYDMPVREAGLEGLVGFIQEFVKWKKFGKMILMGNSLGGHVALLYA
                     LENQDKVTKLVLTGSSGLFENSMGGSFPKRGSYDYIKERVEYTFYDPKTATKELVDEV
                     FETTKSIPKCLRIVSIAKSAQRNNLASRLPELKVPTLLIWGLNDTITPPMVAHDFHRL
                     LPNSKLRFLDECSHAPMMEHPEKFNDILKEFL"
     gene            3415..4089
                     /locus_tag="VGA21_07680"
     CDS             3415..4089
                     /locus_tag="VGA21_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009194489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX9650424.1"
                     /translation="MPEMIAEELINQMIPPLKPSDSANKAIVWMEELRSNQLPVVDQG
                     KFLGFISEEIILETTNIETPVSNFELIGEKCVVNRGTHFYDIIKIASENKVQLVTVLD
                     EDSSYFGVITVQDTIASFAQTAAVQAPGGILVISLSMKDYSLSEISRLIEAEDAKILS
                     SSIKDDDFDKTMLRLTLKINQIDLSRIVATLERFGYKIIARYQESNVTDNQKERLDIL
                     LKYLDI"
     gene            4122..4997
                     /locus_tag="VGA21_07685"
     CDS             4122..4997
                     /locus_tag="VGA21_07685"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010607994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HEX9650425.1"
                     /translation="MRIAIHGKRFIEKTEPVIQQIFDALLQNKAEVILSEEFAAHVKK
                     SGINLMRHKTYDSARGTPSVDTIFSIGGDGTLLETVTHVGKKGTPILGINTGKLGFLA
                     TVSKENIGSAIEKIFKKDYKLDKRTLLNLHSKEKIFSNRTFALNDFTILKRDTSSMIV
                     VHLFLNNEFLNSYWADGLIVSTPTGSTGYSLSCGGPIVLAHSNNFVITPVSGHNLSVR
                     PLVVPDDNELSFKIEGRKRKVLISLDSRYETVDMDIQLKITKADFTISLIRIEGIQFF
                     DTLRQKLNWGYDVRN"
     gene            5064..6152
                     /locus_tag="VGA21_07690"
     CDS             5064..6152
                     /locus_tag="VGA21_07690"
                     /inference="COORDINATES: protein motif:HMM:NF039345.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6089 family protein"
                     /protein_id="HEX9650426.1"
                     /translation="MKVKIGMWVGIILLLFSQLSANAQMTGRSIKNNSNRLSRFRGPN
                     LTFPKYKRYSFAGASINSMNYFGDLAPASNLTSTDIGFTRPGFSLSYGRKYGPIYWWQ
                     MSFSWGTLRGDDFESAGPENETAKYRYVRNLHFRNRISELAFTFHFDLLENPYTYLRR
                     SSWTPYIFTGVAFFHHNPKARVPDYDVYSGREFENAGEWIALQPLGTEGQNTGLYNNK
                     PYARIQPAIPIGLGIKIKLAYRWDLRLEIGMRYLFFDYIDDVSSGYVDLGIFDDPLAR
                     SLSDRSQENTGGASSKPRDFTIIENITSSHTYVGKDGQSYTVYSGYGSDLHPSNIRGS
                     VNDRDIYIITSLKINRILQPYHKTKPFR"
     gene            6160..6840
                     /locus_tag="VGA21_07695"
     CDS             6160..6840
                     /locus_tag="VGA21_07695"
                     /inference="COORDINATES: protein motif:HMM:NF039345.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6089 family protein"
                     /protein_id="HEX9650427.1"
                     /translation="MSSLKTFITILLIALCISTSDAQEGEVGFGIGGLTYTGELERGY
                     NFLNNRPAATVFYRVNLNEALALKFPITFGMLRDSDNNPTDPFASQRNASMNIFLMEG
                     SLQFEYNFLKFRDNIYQRWSPYFFGGIGLFLFSGEGERTTQYSNIQVALPFGMGIKYI
                     MNPRWVVGVEFGFRKTFFDYLDNISEGDPTLKNYQYGNQLENDWYYFLGITLSYTFYK
                     IHCPYPNW"
     gene            6928..7665
                     /locus_tag="VGA21_07700"
     CDS             6928..7665
                     /locus_tag="VGA21_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008203361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEX9650428.1"
                     /translation="MKESLLQGKIPQHIAIIMDGNGRWAKSRGAERIYGHQHAVEAVS
                     QVTEGCAELGVKYLTLYAFSTENWARPKSEVDGLMKLLVSTIRKELKNLNKNNIKLMT
                     IGQIESLPKTCQRELIEAITATSGNTGLTVILALSYSGRWEILEAAKKISRDVSKGRL
                     GLEDIDAHIFEGYLETNFVPDPELLIRTSGEMRISNFLLWQLAYTEIYFTKKMWPDFR
                     KEDLLEAIREYQNRERRFGKISEQVKP"
     gene            7665..10346
                     /locus_tag="VGA21_07705"
     CDS             7665..10346
                     /locus_tag="VGA21_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009194484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HEX9650429.1"
                     /translation="MRKLLFVLAVLLIADNTIAQIRSSRTRPSTSGAELDYAAPKEFE
                     IVQITVTGADFLDKNALISLSGLKVGDKIKIPGDDISQAIKKLWKHGIIGDASIILDK
                     IENGQVYLVIELAERPRLSRMRFDGVNKTQEGDLKDKVKLILGKILTDADLKNIELNV
                     KSYFIDKGFLNTDVEIFQEADSISANGVSLRIAVDKKSKIKINDINIEDNEEVYDTKL
                     QNKMKNTHEKVRFTVFSQVANQVFNFNFRKAGAFVETTREVSGDELKAFMNENVRLNI
                     FRSSKYKKSDYETDKRSIIDYYNSRGFRDAMIVEDTMYAHDDYSINIDITIDEGQKYY
                     FRNITWSGNYVYSDQTLNNILGIEKGDIYDMSLVQTKLTFNPNGPDIQGLYMDDGYLF
                     FNVQPTEVKIDNDSIDLEMRVFEGSQAIINKIIIKGNDRTNDHVILRELRTLPGRKFS
                     RTDIIRTQQRLSQMGFFDPEQIGINPIPNPANGTVDIEYTLVEKPGDQIELSGGWGGS
                     LGFIGTLGLVFNNFSLRNVPKFDKWKPLPFGDGQRVQIRAQANGRRFQNYVLAFTEPW
                     LGGKKPNSFSVSLSRSIQRTYGEGATNSRDLNAKLTLTSIALGLGRALEWPDNYFTLS
                     NTLEYTRYTLNNWPVTNFSSLGFSDGSSNKFALVTNISRYSVDQPLYPRSGSSISLAI
                     TLTPPYSSFNNISYQTAENEEKFKYVEFHKWMFDADYYINLAGKLVMATSIHFGVLGK
                     YSKKAQIGPFERFQLGGDGLAGQNFILSTEIVGMRGYDNDAITPPFGQRELSPDDIRG
                     GIIYNKYSMELRYPLTTGAAATIYFYLFGEAGNNWNNYQEYNPFDAYRSAGIGARLFM
                     PAFGLIGINWGYGFDNVPGTVGRSGPQFQFTIGQQIR"
     gene            10349..10876
                     /locus_tag="VGA21_07710"
     CDS             10349..10876
                     /locus_tag="VGA21_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015028632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HEX9650430.1"
                     /translation="MKTIITFFLTVILLSPPGMAQKFGYVDTDFILKKIPEYRQAQED
                     LNKLSLAWQDEIQALYKDIEGMYNSLQAEEVLLTKEMRDERITAIRKRETEVKEYQKK
                     IFGFEGLFFLKKKELVKPIQDRVFEAVEKVAKEHRLQFVFDKSGELVMIYTDPIHDYT
                     DYVLEELGLGDPIDN"
     gene            10898..11431
                     /locus_tag="VGA21_07715"
     CDS             10898..11431
                     /locus_tag="VGA21_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009580023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HEX9650431.1"
                     /translation="MMKNIIAIALTFLAVGFANAQQNPLKIGYVDVDYILSEMPETKQ
                     IESDLKVHQSQLQNQLQSKMANYQKMIQEYQASAATMTEVIRADKEREITQLEENIQK
                     FQQDAQVSFQKKQSDLLQPVYEKIGKAIEVVANEGSFTYVLSTRIGSIDVILFAIPEN
                     DISDLVLKKMGITPQNN"
     gene            11489..12385
                     /locus_tag="VGA21_07720"
     CDS             11489..12385
                     /locus_tag="VGA21_07720"
                     /inference="COORDINATES: protein motif:HMM:NF036772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEX9650432.1"
                     /translation="MIIPPFLSPGDQVGISATAKKVDQKYLSEGIEILNSWGLNVKTG
                     INVLKEETFFAGNEDERASDLQDFISNPGIKAIFCARGGYGTGRILDKINFSPLLSSP
                     KWIIGFSDITILHLKLNQLGLASLHGPMPAIFHKTTENAIESVRQFLFGGGLKPLFAK
                     YNKENRMGKTSGKAIGGNLSMICHSLGTSLEIDTNNKILILEEVDEPLYKIDRMMHQL
                     GKAGKFEKLRALVMGCFTIPEKESNSFDYSLHEIISYHVRSYNYPIASGFSFGHEPEN
                     YPIPLSVQINIDIGSQGSTISY"
     gene            complement(12382..13617)
                     /gene="chrA"
                     /locus_tag="VGA21_07725"
     CDS             complement(12382..13617)
                     /gene="chrA"
                     /locus_tag="VGA21_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009283977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HEX9650433.1"
                     /translation="MKKVRHYIFLRDVLILSLTSFGGPQAHFAFFLDKFVKLRGYLNE
                     EELVELFALCNILPGPTSTQTVTSIGFRIGGPNLAYLTLLVWITPAVTVMLLAGIFLN
                     HYVEKNASLEFARFIQPMAVGFVSYAAFVISRKVVITNTAVILCVISAIISFSLRKPY
                     IFPILLLMGGFITGFKYRKHPIEEKSPVRIQWANFLLWGGVLLFAALLGGITRERSIL
                     LFENFYRNGSLIFGGGQVLIPYLYTEFVQFKHYLTAQEFLSGYAIAQAIPGPVFSFSA
                     FVGTLSMRDLGVGGEIFGGLMAAFGIFLPGTFLIFFVIRFWEDLKKYRVVRASLEGIN
                     AASAGMVISAALILFFQPFNAAFTTPDWVEVLSQLFLLLWNSPVYLGLVLLTFLILRY
                     TKIPAPYIIISGLIAGFLF"
     gene            complement(13638..14609)
                     /locus_tag="VGA21_07730"
     CDS             complement(13638..14609)
                     /locus_tag="VGA21_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019596246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate and iron-dependent oxygenase
                     domain-containing protein"
                     /protein_id="HEX9650434.1"
                     /translation="MQPVFYDEIPSLDLSDFTGSNKARKQNFVNDLGRAYQNIGFVAV
                     KNHGVSDELTAELYAIVKQFFNLPDETKRKYEIPELSGQRGYVGKGKEHAKGRSTGDL
                     KEFYHIGQEVEGDDPIKAEYSDNIWVDELPQFRKIGLEVYRKLEQAGIQMLHAIALYL
                     GLEENYFDNKVKNGNSILRPIHYYPIENPDAVPADAVRAAEHGDINLITLLMGASADG
                     LQVLRRDGHWIPITALPNQIVVNVGDMLERLTNKKLISTIHRVVNPSREKMSTSRYSI
                     PFFMHPRSEMDLSCLENCIDDENPRIFQDITAGEFLNERLAEIGLKK"
     gene            complement(14614..15081)
                     /locus_tag="VGA21_07735"
     CDS             complement(14614..15081)
                     /locus_tag="VGA21_07735"
                     /inference="COORDINATES: protein motif:HMM:NF025705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4399 domain-containing protein"
                     /protein_id="HEX9650435.1"
                     /translation="MKKLTFILFTFTVTFWIISCSGTGSSEQESSETDSVSTEMTSAK
                     VYFISPNDGDTVSSPLMIDMGLEGMEIDPAGEVKEGMGHHHLVINGSHIQEGVVVPTD
                     SVHIHYGLGQIRDTVELAPGNYTLTLQFADGFHQSYGKPLSSTINVVVKDNNQ"
     gene            complement(15130..15813)
                     /gene="recO"
                     /locus_tag="VGA21_07740"
     CDS             complement(15130..15813)
                     /gene="recO"
                     /locus_tag="VGA21_07740"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEX9650436.1"
                     /translation="MISKTSGITLGKIKFRETSIIARIFTEKFGLQSYLINGIRSPKG
                     KFKIALFQPLTLLDLVVYYKENAGIQRLSEVKCSYPYSTISGNIKKSVIFIFLTEVLE
                     KTLRDCSNPEEVFAFLQQSLIKFDTENENQSSFHLRFLIEFSAILGFKPQSIIDFKNH
                     IPSNLHASGRDALLLIKQMIENKTQKISVSSNSTRRNCLNLIIAYYRGHIDNLGEIKS
                     VGVLKEILD"
     gene            complement(15821..16417)
                     /locus_tag="VGA21_07745"
     CDS             complement(15821..16417)
                     /locus_tag="VGA21_07745"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010856581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEX9650437.1"
                     /translation="MFIEPHSGRSNPGNTRGGWIEVICGSMFSGKTEELIRRLNRALI
                     AKQKVEIFKPAIDKRYHEEDVVSHDERAIRSTPVNFPSDILLLTGECDVVGIDEAQFF
                     DDSLTEVIVELANGGKRVIIAGLDMDFEGKPFGPLPSIMSVAEYVTKLHAICARCGSL
                     ASYSFRLSGLNEKVVLGEKGHYEARCRKCFLEGMSSNK"
     gene            complement(16423..16743)
                     /locus_tag="VGA21_07750"
     CDS             complement(16423..16743)
                     /locus_tag="VGA21_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002694944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEX9650438.1"
                     /translation="MARIIIQNFYQKEIIFKPSGKNVLKILLQSYVDWLHSCGGKGRC
                     TTCKMVVVSGSQNLNPLTTAEKNYKRLRQLNQNERLACQAIPSGDLTILVPKDNQLPH
                     LKYS"
     gene            16778..17752
                     /locus_tag="VGA21_07755"
     CDS             16778..17752
                     /locus_tag="VGA21_07755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650439.1"
                     /translation="MENKLNLESTIKYSEEFSKILCDRFFSTEKRITGEQILNLTNIR
                     QINLFVIRILFSKWQKEQGKIKSSYFDYTNHEIQKALKDFLNKLSQHISVAKEDFYPL
                     LKLACIKTIQVIYAPYDFFMQEISGDSDDNINVEKLKESSKYLKINNGFFNELLQRLN
                     QSGAKELSSTEARGILEEVFLNYSGSPEDGEAYLAHFNEVLELRVEDLFTPEKEVKPA
                     ENETSYSTINDDHANRIGETIGEALKKISKTNLQESVSVNQKFMFVHVLFNGNPKLFE
                     EALGKIQEFDSKEQAMQFLEKELKNTYQWDKESEEVGEFYDLVLTRFD"
     gene            complement(17749..19017)
                     /gene="rodA"
                     /locus_tag="VGA21_07760"
     CDS             complement(17749..19017)
                     /gene="rodA"
                     /locus_tag="VGA21_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015332173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HEX9650440.1"
                     /translation="MQRSENLVSSIDWLTILLYFLIVLLGWLNIYAAVYDEGTTGSIF
                     DFSLNSGRQLVWIGASVIIIIFILIIDFKFYSTFAYFIYGAVFVVLLGVLGFGTEIAG
                     SKSWIRLGSFGVQPSEFMKFAAALAVAKFMDSHGVKFNNFKDLFILGAFIALPMGLIV
                     LQGDTGTALIFGAFILVFYREGLSPLFLIFVAASAAIFLLALLVTQSYLAIGIGASAF
                     LIIMLSKKNLKTITGILLGAAIVFSLIRSVDYVFTNVLKPHQQNRIKALINPEEDPLG
                     YGWNVTQSKIAIGSGGMWGKGFLEGTQTKFDFVPEQSTDFIFCTIGEEHGWFGTLILI
                     TLFMLLFLRIVYISERQKTRFARVYGYSVVAILFFHFAINIGMTIGLFPVIGIPLPFF
                     SYGGSSLWAFTCLLFVLIKLDAHRMDQLVH"
     gene            complement(19004..20809)
                     /locus_tag="VGA21_07765"
     CDS             complement(19004..20809)
                     /locus_tag="VGA21_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009578701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEX9650441.1"
                     /translation="MIEGRKYVIQAVFLITGIAFLVKLFTIQVTDKTYAKAATDNIIL
                     PVVEYPYRGLIFDRNGELIVYNNPQFDLTVIPQEVKQLDTLSICEILGLTREDFKQRL
                     SAARKYSYVKPSPFFKMISNQEFGKIQDKLVDYPGFGIQARTSRAYAHPVLSNSLGYI
                     AEISSAGLRQDSSRYYRQGDYIGKSGLELYYESQLRGKRGISYKIKNVRGIEKGSFED
                     GEFDTIPIPGQNLVSTIHLRLQLYAEKLLKGKVGSVVAIEPATGEILAIVSGPSYDPG
                     MMVGRKYSSNFSLISRDSLNPLFNRPVMAMYPPGSIFKIVQSLIGLQENVLNVNETIY
                     IDGRNIGDHAPPGYYTLHEAIRKSSNNYFFMAFRRMINQNLNANTFIDSRLGLDNWKS
                     HVMSFGFGAPLGIDIPNEKGGFIPGSDYYDKIYGHNSWKFSTISSLSIGQGEMLLSPL
                     QMANLAAIMANRGYYYTPHLVKEIGDYGPLSKFTEKHMAGIDEKHFPPVINAMQDVVT
                     STAWRAVIRDIEICGKTGTAENPHGEDHSVFMAFAPKKNPQIAISVYVENSGWGGRAA
                     ASIASLLIETYIRGNITRQWLEDYVLKGDFADATQ"
     gene            complement(20806..21330)
                     /locus_tag="VGA21_07770"
     CDS             complement(20806..21330)
                     /locus_tag="VGA21_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009195266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rod shape-determining protein MreD"
                     /protein_id="HEX9650442.1"
                     /translation="MDTRSLILTMLNFIVFILVQTLIFKNTVLFGVSFAFLYVAFLLF
                     LSFETTAVYLLLAGFFTGFVIDIFYDSLGVHTAACVLMMFARQFWIKSIIPRGGYEIG
                     STPTIASLGLQWFTIYALPLIFIHHFLLFYIEAGGFKGLFFFTLSKVLASMVFTYFTV
                     ILYQYLFFVRKNRA"
     gene            complement(21330..22148)
                     /gene="mreC"
                     /locus_tag="VGA21_07775"
     CDS             complement(21330..22148)
                     /gene="mreC"
                     /locus_tag="VGA21_07775"
                     /inference="COORDINATES: protein motif:HMM:NF010532.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEX9650443.1"
                     /translation="MYRLYLFLFKYRVTLIFIFLEVLSFWLIVQNNRYQSAAFFNSSN
                     AISANLLSISSEIRGYFSLKKTNTQLAEEIANLRKELERKKVAPVKGIVQDSAYIPYE
                     FVTAKVISNSAYNFNNYLTINRGNAEGVKPDMAVIGPNGIVGKIKYTSENFAVVTSLL
                     HSNFLISSKVEGKINLCSTQWDGVSPSFAQLLFVPTHIVLLEGDTVSASGFNSIFPED
                     ILIGTVSKISLREGAPFHEIEIKLSTEFANLSFVQVIISAIRDELDSLGVNERF"
     gene            complement(22156..23181)
                     /locus_tag="VGA21_07780"
     CDS             complement(22156..23181)
                     /locus_tag="VGA21_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010609813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEX9650444.1"
                     /translation="MGLFDFFSSDIAIDLGTANTLIIFKDKIVVDEPSIIAINKTNNK
                     VLAIGREAMQMHEKTHENIKTIRPLKDGVIADFHAAEHMIRGMIKMINMGNRWFPSSH
                     RMIICIPSGITEVEKRAVRDSAEHAGAKEVYMIHEPIAAAIGIGIDIEQPIGSMIVDI
                     GGGTTEIAVIALSGIVCDQSIRVAGDTFNRDILDYMRRQHNLLIGERSAEKVKIEVGS
                     ALTELDDGPDDYEIRGRDLMTGIPKVIKVSYSEIAFALDKSVSKIEEAVLKALEISPP
                     ELSADIYDNGIHLTGGGALLRGLDKRLALRTKLPIHVADDPLRAVVRGTGMALKGLDQ
                     YIGVLMT"
     gene            complement(23272..24798)
                     /gene="purH"
                     /locus_tag="VGA21_07785"
     CDS             complement(23272..24798)
                     /gene="purH"
                     /locus_tag="VGA21_07785"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009184509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEX9650445.1"
                     /translation="MTIRKINTALISVFHKDNLESIVLRLHELEVEIYSTGGTQTFIE
                     QLHVPVKSVESLTGYPSILGGRVKTLHPKIFGGILSRRENESDQNELSNYEIPALDLI
                     LVDLYPFEETMAAGASHKEIIEKIDIGGISLIRAAAKNYQDTLIISSREQYKELLDIL
                     NLSKGETTPEQRKLFATRGFEVTSHYDTAIFNYFNREEGVRSFKQSLRESKALRYGEN
                     PHQQGIFYGSLNDTLEQLHGKELSYNNLVDIDSALNLLEEFENETVFIIIKHTNACGL
                     ATAASVAEAYQKALAGDPVSAFGGILATNKTLDESAASLINDLFFEILIAPAYSAKSL
                     QILQSKKNRILLKQKSKITGSKQFRNLVNGVIEQDRDLKTDSVEDLKVVTKVHPNKEQ
                     IAGMLFASKIAKHTKSNTIVLAVDGQLLASGVGQTSRVDALQQAITKAGANKFDLKKA
                     VMASDAFFPFPDCVEIAHNAGIIAVIQPGGSIKDQDSINFCNSHGMTMAFTGIRHFKH
                     "
     gene            complement(24836..25063)
                     /locus_tag="VGA21_07790"
     CDS             complement(24836..25063)
                     /locus_tag="VGA21_07790"
                     /inference="COORDINATES: protein motif:HMM:NF012760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HEX9650446.1"
                     /translation="MYGARVHQAVSESGDTESGITIHYVNEHYNEGDIIFQTSCSIRA
                     GENPKSIAHKAHQLEYEYYPKVIEEVLNKLG"
     gene            25599..26132
                     /locus_tag="VGA21_07795"
     CDS             25599..26132
                     /locus_tag="VGA21_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015329769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX9650447.1"
                     /translation="MDSCRGGNRNSQDQLYREFYPYAIGICLRYSRTRDEAIEILNDG
                     FLKIFTKLDHYTKGLSFTGWLRKVMINSAIDYYRRNEKHYNALDISHVQYEAVSETAL
                     DALSAEEIIAAVQKLPPSYRMVFNLFVIEGYKHVEIANMIGISVGTSKSNLAVAREKL
                     KKMLIIENDKLGKEKNG"
     gene            26125..27393
                     /locus_tag="VGA21_07800"
     CDS             26125..27393
                     /locus_tag="VGA21_07800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650448.1"
                     /translation="MDDRKFDELLKSKIGEYRNPGFEPSALAALHQKMAAISQPWYGR
                     YRTELIAGTATLLCAGMIFLGQYFSNRVNEQWMATNIQALKDQENQIQLLKEEIERLR
                     NLAPDTIRITEFSGQDNSGYYLLLLSKISALERKLETLGNPENQMRNSQLIPLADYNA
                     GKFSNSLIFDKNESMRIHSISRLNTRNPEVSRAEGKIEVSPKEKESVNLSAKTIRDIE
                     KHYMRGVGIRVGPAVEIHRGFYSEGEGAYNFGGGIAADFIMSPSLSLETGVKYALHTN
                     KVKNADSSNLNFPDVDPQLGQLNTAEIDFYSLELPLYLKYRYPFSDKTYLQTGVGYSF
                     FIYTKEEFDYDYEISGNPGASINTLVKDKNFTIYPGTLNFSLGLSKELKNKKILETSL
                     FYQHGVGSMGIEKIKLQFLGVKGSYWFKIR"
     gene            27508..28887
                     /locus_tag="VGA21_07805"
     CDS             27508..28887
                     /locus_tag="VGA21_07805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650449.1"
                     /translation="MKTRLSRLLTFGLIVFMTLNVFSCGEDNPLPIFEDDILLVAANL
                     NPADFASEINNPYFTLLTGDIHIYEGVDEDGVEERVVSRVTFETKTILGVNCVVVLVK
                     EYSDGSLIEETRDWYAQDKKGNVWYFGEDVKDIEDGQVYSTFGSWEAGVNGAKPGIIM
                     LANPQPGRKYHQEVAVGIAEDQAEVVSLNNTVTVPYGTFQNCLKTKETNPLDPGFEEF
                     KYYAPDVGFIKAEPAAGGVEELAEITDMVDRALLNAGNINPVMFVEGVDNPYFPLTPG
                     KVYTYSGLNDEDLPVTVISEVTTETKVILGVVCTVVHEKEFEDGELIEDTYDWYAQDV
                     EGNVWYFGEDTKSIEDGVVVGTGGSWEAGVNEAVPGIIMLANPIPGMKYRQEVYEGEA
                     EDWAEVSSINNTVTVPFGTFENCIKILTSTPLEAEVLEVKYYAPGIGFIKGEDQTSSE
                     FEALENISN"
     gene            28963..31266
                     /locus_tag="VGA21_07810"
     CDS             28963..31266
                     /locus_tag="VGA21_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015056696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX9650450.1"
                     /translation="MKGFKPNHFLAFPLILMILNSGAYSQEKFTLSGIITDEETGEAL
                     IGANIYVTTLEKGTSTNTYGFYSLTLPRTDSLGIIFSYIGYIPQIKKIYADKNLELDI
                     EMKPSTSTLEEVTIQGQQDANVARPQMSVVNVPLEKIKELPVILGEIDVLKVIQLLPG
                     VQSGNEGTTGIFVRGGNADQNLVLLDEATVYNPSHLFGLFSTFNSRALNNVTLIKGGF
                     PAQYGGRLSSILDISMKEGNNRKFQGEGGIGLVTSQLTLEGPIKKEKASFIVSGRRTY
                     IDLILTPFLEVGNSSDYHFYDINGKVNWKISSKDRLFFSFFTGKDDALYNETKGISYN
                     IKFGNSAATLRWNHLFGQKLFLNTSLIYNTYEQDIATIQDNFFSQVVSGINDVNGKME
                     FQYFPSLNHEIRFGAHYTNHRFLSGGKSEAQTSGNQTINVSNLPSKYFSEYAFYVNDE
                     FNIYRGFSANLGFRFPGFSDSDVNYFEWEPRATLKLQLGPTSSVKAAYTVMHQFLHLV
                     PSSTASVPTDIWLPSSKRTKPQLSEQFAIGYFRNFNQNQFETSAELYYKTMDNQVLFR
                     EGNQLIESLEVDTALVYGKGESYGLEFFLRKNTGPLTGWLSYTLSKTDQKFDSLNFGR
                     TFPFQYDRRHAVSLAIAYKINPRWTVSAVFEYATGKVKTLPVGRINSYYGGSLYEGTF
                     YVYEERNNARLKDYHRLDISATYKRNRKIFRKEYVAELVFGVYNIYSRQNPYFVYFLV
                     DPVTDQPKAKQVSLLPILPSLSYNFKF"
     gene            31272..>31911
                     /locus_tag="VGA21_07815"
     CDS             31272..>31911
                     /locus_tag="VGA21_07815"
                     /inference="COORDINATES: protein motif:HMM:NF025420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4249 family protein"
                     /protein_id="HEX9650451.1"
                     /translation="MKPQKLSSIFILMLVVAACEREININPSTGFTGDIFIEGILYPG
                     EFPRVFISRSPGFFEPDATPQELFGRGAVVTITGSMGQEILAADSTFDKFRCRWVPFY
                     AGNTPAIYGEAYDLTVIYQGKSYSASTTINQPKPIIDTVEYVGEFFDIYGGHDGVIIT
                     FTDSPGSGDFYRYQMNRMIDRNTHHVDVLEVIVNDCVAEGELFRVTDLGRSVF"
BASE COUNT         9968 a         6282 c         6644 g         9017 t
ORIGIN      
        1 ccccgtcacc accggcataa ttatcagtat cattcttaca ataatccttt acgtaataag
       61 ccggtttcgc taagacttca agaagtcatt taagagggta ataaataact cgggttgttc
      121 cgcgtgtacc cagtgcccgg catcttttat tactttaatt tcagaatttg ggaatcttgc
      181 tttaattaaa tcataatcct gttcgttaat gtagtttgat ttttcacctt taatgaacaa
      241 agtctttttt tcaaatttat catttccggg aagtccgatt ccgacccatt ccattttcat
      301 tcttataacc ggcaaattta ttttccatgt gaaatttcca gtttcatctc tgctcagatt
      361 tttcaaaaga aaattccttg ttgtggaatc actcacatat tttgataatt gtgtatcagc
      421 atcattgcga gattgcaatt tgtccaattc aatagaagat aatccctcaa ggatattatc
      481 atgatggata ggataagaac gaggtgatat atccacgata acaagttctt ttaaaagttc
      541 cgggaatgaa atggcaaaat tcattgcgac tttaccaccc atagaatgac caataatgaa
      601 ggggtttaca atttcatgct ccgcaataaa tttttttaag tcattaacca ttgaggggta
      661 gtcaaattta tcagaatgaa aagactgtcc gtgattacgc tgatccaggg tgaagacctt
      721 gtaatctgaa gacagagacc tggctacggt catccaatta tcggaagaac ctaatattcc
      781 atgcaaaata ataatagggt ctcctgttcc ctgctcccgg taaaataatg aaggctgatt
      841 cattgtaact ccttaagata taactttatg gcattatcga gccctaaata aagtgcatca
      901 caaataaggg catgcccgat ggagacttca tcaataaaag ggatggaagt tttcaaaaat
      961 ttcaggttat ccaggtccaa atcatgcccg gcatttatac caagtccaag ttcgttggcc
     1021 agttcagcag ccttaatata gggagcaata gcttcggcac gatctgacga ataccctttg
     1081 gcataagctt cagtatacag ctcaattctg tcggttccgg ctttcgcagc tgcttctatc
     1141 atttgcaaat ccggctcaat aaaaatggaa accctgactc cgaagccttt caatttcctg
     1201 attgtactga ataaaaaatc cttctctttg atagtatccc acccggcatt ggaagtcaaa
     1261 acatccggag ggtcgggaac caacgtagcc tgggcaggcc ttacggtctc aataatttga
     1321 agatatcttt catcaggata tccttcaata ttgaattctg ttttaacaat atcttttaaa
     1381 tccatcacat ccttatattt aatgtgtctc tcatcaggac gaggatgcac ggtaattccc
     1441 tgggcaccga atctttcaca atcaagggca atttgcaaaa gatcaggatt gtttccacca
     1501 cgggcattac gtaatgtggc tatcttatta atatttacgc tcaggcgcgt cataaattct
     1561 caattgatta actttagcct aaattaattt attcagaaga attttttacg atgagcctaa
     1621 aacaaagaat tgatgctgaa attaaaaagg ccatgcttgc caaagaaagt gatcgactgc
     1681 gggctttaag aggtattaag tctttgatac tccttgcaga atcggaaaaa gaaaacagaa
     1741 gcggtctttc tgagaaggtg gagacacaaa tactcactaa agcagcaaag cagagaaaag
     1801 attccgcgga gatttacaat cagcaatcca gagacgatct cgcgggaaag gagatttttg
     1861 aattaaaagt aattgaggaa ttcttgccgg agcagatgtc agaaaaagaa attgaaagac
     1921 aagtttctga aattatctcg gaaaccgcgg cggaaggaat gaaggatatg ggcaaagtta
     1981 tggccattgc gactaaacaa atggctggca aagcggacgg ccgaataatt tctgaaattg
     2041 taaagaagaa gttatcataa attgaacagc ctagattgga taattctggc cttcctcact
     2101 tttgcagcct tccaggggta ccggaaggga tttattctgg agataatttc agtccttgca
     2161 cttattctgg gtatcattgg tggcttaaaa ttgatcgatt ttggtaaaga atttctatcc
     2221 aggcaccttg aaataaaggg tgacatacta ccctatctca gcttcttatt tattttcata
     2281 attatcgtta ttctcttaaa tctggccggt agagcggtta agaaaattct ggatatgacc
     2341 ttgcttggca acctggatga cattgccgga ttagtaatcg gaattctaaa atggaccctc
     2401 atcctttcat ccttgatttg gattgcttct ataattgatc tgaagctgcc attcacctgg
     2461 accgaagatt caatacttta tccctatgtt tcatggcttg ccccaaacct gtttcatatg
     2521 ctatcgggag tttttccatt tttggaagaa ttgattgcat attttgcctc atgaattgat
     2581 tttgtcctct gttcacaatt cactatttta ccacaaggtc aaatgatact tatttaaatc
     2641 acttattttt gattcaaaga atgggaaaag ggttcaatct gaaagttgac aatgggttta
     2701 cctacattga ggaaggtgaa ggcgaagtaa tactgctctt gcacgggctg tttggtgccc
     2761 tgagcaactg gaaatgggta ctggaacact tctcttcaaa ttacaaagta gttataccca
     2821 tgctcccaat ttatgacatg ccggtgaggg aagccggttt ggaaggatta gttggcttta
     2881 ttcaggagtt cgtgaaatgg aagaagttcg gtaaaatgat cctgatggga aattccctgg
     2941 gtggacatgt cgcgcttctg tatgccctgg aaaatcagga caaagtaaca aaactggttt
     3001 taaccggcag ctcaggattg tttgaaaaca gtatgggcgg ttcttttcct aagagaggaa
     3061 gctacgatta tataaaagaa agagtagaat acacatttta tgatcctaaa acagccacaa
     3121 aagaattggt ggatgaagtt tttgaaacca ccaagagtat ccctaagtgc ttgcggattg
     3181 tttccattgc aaaatctgcc caaaggaata atttggcatc caggctgccg gaattgaaag
     3241 ttcctacttt actgatttgg ggtttgaatg ataccatcac gccacccatg gtagcacatg
     3301 actttcatcg tttactgcca aattcaaaac tgaggtttct ggacgaatgt tctcatgctc
     3361 caatgatgga acatcctgaa aagtttaatg atatattgaa agagtttttg taatatgcct
     3421 gagatgattg ctgaagaact cataaatcaa atgatcccac ccctgaaacc atctgattca
     3481 gccaataaag caattgtttg gatggaagaa ctgagatcta accagctgcc ggttgtagac
     3541 caggggaagt ttttaggctt catttctgaa gaaataatac ttgaaaccac taacattgaa
     3601 acccccgttt caaattttga attgattgga gaaaaatgtg tggtaaacag gggaactcat
     3661 ttttacgata taattaaaat tgcttcggaa aataaggtcc agctggtaac cgtacttgat
     3721 gaggactcct cttatttcgg agttattact gttcaggaca ccattgcttc attcgcacaa
     3781 actgcggcgg ttcaggctcc gggcggaatt ttggtaattt ccctaagcat gaaagattac
     3841 agcctgtcag aaattagccg gctaattgaa gcagaagatg ctaaaatctt aagcagcagt
     3901 attaaagatg atgattttga taagaccatg ctgcggctta ccctgaaaat aaaccaaata
     3961 gatttgtcaa ggattgtggc cactttagag cgatttggat ataaaataat agctcgctac
     4021 caggaaagta atgtcaccga caatcaaaaa gaaagacttg atattcttct taagtacctc
     4081 gatatatagt atcccgaaat caggaataat caaattaaaa aatgagaatt gccattcatg
     4141 gtaaaaggtt catagagaaa accgaaccag ttattcaaca gatttttgac gctttgttac
     4201 agaataaagc agaggtgatt ttatcggagg agtttgcggc ccatgttaaa aagtcgggca
     4261 ttaatttgat gcggcataaa acatatgact cggccagggg aacaccatcg gtagatacaa
     4321 ttttttcaat cggaggagat ggaacgcttc tggaaacagt tacccatgtt gggaaaaaag
     4381 gcacaccaat tctaggtata aataccggca agcttggatt cctggctacc gtatcaaaag
     4441 aaaacatagg aagtgcaatt gaaaagattt ttaaaaaaga ttacaagctt gataaaagaa
     4501 ctcttttaaa cctgcattca aaagaaaaaa ttttttcaaa tcgtaccttt gccctcaacg
     4561 attttactat cctgaagcgg gacacctcct ctatgatcgt agttcatctt tttctcaata
     4621 atgaatttct taattcttat tgggcagatg gattaattgt ctccactcca acagggtcaa
     4681 ccggatactc cttaagttgt ggaggaccga ttgttctggc ccattccaac aactttgtta
     4741 taacaccggt aagtggtcat aatctgtcag taagaccttt ggttgtacca gacgataacg
     4801 aactttcctt taaaattgaa ggaagaaagc ggaaggttct tatttcactg gattccaggt
     4861 atgaaactgt ggatatggat atccaactaa aaataaccaa agcagatttc acgatctcac
     4921 taatcagaat agagggaatt caattttttg atacgctcag gcaaaagctc aattggggct
     4981 atgatgtaag aaattgattg aattgaattt ttcatataaa taattttgta ttatttatgc
     5041 aaaaatgatt tattcaagat acaatgaagg tgaaaattgg gatgtgggta ggaattatct
     5101 tattactttt cagtcagtta tctgcaaatg cccaaatgac ggggcgatcc attaagaaca
     5161 atagcaacag attaagccga tttaggggcc caaatcttac atttccgaaa tataagcgct
     5221 atagctttgc aggtgcttcc attaattcta tgaactactt tggagatctg gcaccggctt
     5281 ccaatcttac cagcacagac attggattta cgagacctgg attttcacta tcatatggta
     5341 gaaagtatgg tccaatctat tggtggcaaa tgtctttttc ctggggaact ctgcgaggcg
     5401 atgactttga atctgccggt cctgagaatg aaactgccaa atacagatac gtcaggaatc
     5461 tccactttag aaacaggatt agcgaactcg cgttcacttt tcattttgat ttacttgaaa
     5521 atccttacac ttaccttcgg aggtcaagtt ggacaccata catttttaca ggagtcgcct
     5581 tcttccatca taatccaaaa gcaagagttc cggattatga tgtgtattcg ggacgtgaat
     5641 ttgaaaatgc cggggagtgg attgcactac agcccctggg aacagaagga caaaacacag
     5701 ggttatacaa caataagcct tatgccagga tacagcccgc cattcctatt ggattgggaa
     5761 taaaaattaa attggcatat cgatgggatc tcagattgga aatcggaatg aggtatttgt
     5821 tttttgatta tatcgatgat gtaagtagcg gctatgttga tttgggcatt tttgatgatc
     5881 ccctggcaag gtccttatcg gaccgatctc aagaaaatac aggcggcgca tcgtcaaaac
     5941 ccagagattt tactattatt gaaaatataa cttcatcgca tacctatgtt ggaaaagacg
     6001 gacaatctta tactgtatat agtggttacg gaagtgattt gcatccttcc aatatcagag
     6061 gtagtgtgaa tgacagggat atttacataa tcacgagtct caaaataaac agaatacttc
     6121 agccatacca taaaacaaaa ccatttcgtt aattaacaga tgagttccct caaaaccttc
     6181 attacaatcc ttctgatagc actttgtatc agtacaagtg atgcgcagga aggtgaagtc
     6241 ggctttggta taggcggcct gacttacacg ggagaattag aacgggggta taatttcctt
     6301 aataacagac ctgcagcaac cgtgttttac agggtaaacc taaacgaagc acttgcactt
     6361 aagttcccca ttacatttgg aatgctgcgg gattccgaca ataacccaac cgaccctttt
     6421 gcttcgcaga gaaatgcttc tatgaatatt tttcttatgg aaggttcgct tcagtttgaa
     6481 tataattttt tgaaattcag agataacata taccaacgat ggtcccccta cttctttgga
     6541 gggattggtc ttttcttatt ttcaggagag ggcgaaagaa ccacacaata cagcaatatt
     6601 caggttgccc ttccatttgg gatgggaata aagtacatta tgaatccacg gtgggttgtc
     6661 ggagtggaat ttggattcag aaaaaccttt ttcgattatc tggataatat ttctgaggga
     6721 gatccaacct tgaagaacta tcaatatgga aaccagctgg aaaatgactg gtattatttt
     6781 cttggaataa cgcttagcta cactttctat aaaatccatt gcccctaccc caattggtaa
     6841 gtacttaatc agattttgcc tattctgaca aaaaaacacg attttttaac caattcgaac
     6901 taattttgcg tgtctaattg attgaggatg aaagaatcat tacttcaagg caagatacct
     6961 cagcacattg cgataattat ggatggcaat ggaagatggg ccaaaagtag gggagcagag
     7021 cggatttatg gtcatcagca tgctgtggag gccgttagcc aggtaactga aggatgcgca
     7081 gaattgggag tcaaatatct cacattatat gcattctcta ccgaaaactg ggccagacca
     7141 aaaagtgaag tggatggctt gatgaaattg cttgtatcca caatcaggaa agaattaaaa
     7201 aatctgaata aaaataacat taaattaatg accattggac aaattgaaag tcttccaaaa
     7261 acttgccagc gggaattaat tgaagccata acagccacat ccggaaacac tggacttacg
     7321 gttattcttg cactcagcta tagtggccgg tgggaaattc tggaagcggc taagaagatt
     7381 tctcgcgacg tcagcaaagg gagactggga ttggaagata ttgatgccca tattttcgag
     7441 ggatatcttg aaactaattt tgttcctgat ccggaattat taataagaac aagcggggag
     7501 atgagaataa gtaacttttt attgtggcaa cttgcataca ctgaaattta tttcacaaaa
     7561 aaaatgtggc ctgatttccg caaagaagat ctcctcgaag ccattcgcga atatcaaaac
     7621 cgggagagaa gatttggaaa gattagtgag caagtaaaac cctgatgaga aaactactat
     7681 ttgtactggc agtactccta attgcggata atactattgc acagattcgt tctagccgga
     7741 ccagaccttc cacctccgga gctgaacttg actatgcagc acccaaagaa tttgaaatag
     7801 tgcagattac agttaccggc gcggacttcc tggataagaa cgctttgata tccctatccg
     7861 ggttaaaggt gggagataaa atcaaaattc ccggtgatga tattagccag gcaataaaga
     7921 aattatggaa acacggaatt attggagatg cttccataat tctggataag atcgaaaatg
     7981 gccaggttta cctggttatt gaacttgctg aacgaccaag attgtcgagg atgagatttg
     8041 acggtgtgaa taaaacccag gaaggagatc ttaaagataa agtaaaactg attcttggaa
     8101 aaatacttac tgacgcggac ctgaagaata tagaactgaa tgttaaatcc tattttattg
     8161 ataagggttt tctgaacaca gatgtagaaa tatttcagga ggcagattcc atatcagcaa
     8221 atggagtttc tctaaggatt gcagtggata agaaatccaa gatcaaaatc aatgacataa
     8281 acattgaaga taacgaagag gtttatgata ccaagctaca aaacaagatg aagaacactc
     8341 atgaaaaggt gagattcaca gtcttcagcc aggttgctaa tcaggtattt aatttcaact
     8401 tcagaaaagc aggagcattt gtggaaacaa ccagggaggt aagcggagat gagttgaagg
     8461 catttatgaa tgaaaatgtg cgattgaata tttttcgttc atcaaaatac aagaagagtg
     8521 attatgaaac ggacaaacga agcataatcg attattataa ttcaagaggt tttcgtgatg
     8581 caatgattgt ggaagacaca atgtacgctc atgatgatta ctccataaat attgacatca
     8641 ctattgatga agggcagaaa tactatttca gaaacataac ctggagcggc aattatgttt
     8701 attcagatca aaccctcaac aatattctgg gaatcgaaaa gggtgatatt tacgatatgt
     8761 cattggttca gaccaaactg actttcaatc caaatgggcc tgatatccag ggattatata
     8821 tggatgacgg ttacctgttt ttcaatgtgc agccgaccga agttaaaata gataatgatt
     8881 caattgattt ggagatgagg gtttttgagg gatcccaggc catcatcaac aaaataatta
     8941 ttaaaggaaa cgaccgcaca aatgaccatg taattctcag ggaattgaga acattgcccg
     9001 gaaggaagtt cagcagaacc gacattataa gaactcaaca gcgactttcg cagatgggat
     9061 tttttgatcc cgagcaaatt ggtatcaacc cgattccaaa tcctgccaac gggacagtgg
     9121 atattgagta tactttagtt gaaaaacccg gagatcagat tgagctgtca ggaggctggg
     9181 gtggttctct cggtttcatt ggtacgctcg gcttggtttt taataatttc tcattgcgga
     9241 atgtgccgaa atttgataaa tggaaaccct tgccttttgg cgatggccag cgggtacaaa
     9301 tacgggctca ggccaacgga agaagatttc agaactatgt tctggctttt acagaaccgt
     9361 ggcttggcgg taagaaacca aattcatttt cagtaagcct gagccggtct atacaaagaa
     9421 catatggtga aggcgcaacc aacagcagag acctgaacgc aaagcttaca ttaaccagta
     9481 tcgcacttgg ccttggccgg gccctggaat ggccggataa ttattttacg cttagcaata
     9541 ctttggaata taccaggtac acccttaata attggcccgt tacaaacttt agtagtttag
     9601 gtttttcaga cggctcatca aacaagttcg cgttggtaac caacatttca aggtatagtg
     9661 tagatcaacc attgtatccc cgctcgggat cctccatttc tttggcaatt accttaacac
     9721 caccctattc ttcattcaat aatattagtt atcagactgc tgaaaatgaa gaaaaattca
     9781 agtatgttga atttcacaaa tggatgtttg atgcggatta ttatataaat ctggcaggga
     9841 aattggttat ggcaacaagc atccatttcg gagttttggg caagtatagt aaaaaagctc
     9901 agatcgggcc ctttgaaaga ttccagctag gtggtgatgg attagcgggt caaaatttta
     9961 ttttgagcac agagatagtt ggaatgagag gttatgataa cgatgcaata acgccccctt
    10021 tcgggcaaag ggaattgtct cccgatgaca taaggggagg tataatttat aacaagtatt
    10081 caatggagct tagatatccg cttaccaccg gagccgccgc tactatttat ttttatttat
    10141 ttggagaagc cggcaacaac tggaacaatt atcaggagta taatccgttt gatgcttata
    10201 gatctgcggg aattggagcc cggcttttca tgcctgcttt cggattaatt ggtatcaact
    10261 ggggatatgg atttgataac gttcccggaa ctgttggcag aagcgggccg cagtttcagt
    10321 ttaccattgg acagcaaata cgatagagat gaaaactatt attacttttt tcctgacggt
    10381 tatcttgctt tccccgcctg ggatggctca gaagtttggt tacgtggata ccgatttcat
    10441 actcaagaag ataccggaat acagacaggc tcaggaggat ttaaataaac tatctttggc
    10501 atggcaggac gaaattcagg ccttatacaa ggatattgaa ggtatgtata atagcttgca
    10561 ggcggaagaa gtacttctta caaaagaaat gagagacgaa agaattactg ccatcaggaa
    10621 aagggaaact gaagtgaagg agtatcaaaa aaagattttt gggtttgagg gtttattttt
    10681 cctaaagaaa aaagaacttg ttaaacctat acaggataga gtttttgaag ccgtagagaa
    10741 agtggcaaag gaacacaggc ttcaattcgt atttgataaa tcaggagagt tagtaatgat
    10801 atatactgat ccaattcatg actacaccga ttatgttttg gaagaacttg gattaggtga
    10861 ccccatagat aattaattat tgacagaaaa tttcaaaatg atgaaaaata taattgcaat
    10921 cgcacttaca tttttggcag taggatttgc caatgctcaa caaaacccat taaagattgg
    10981 atatgtcgac gttgattaca ttttaagtga aatgccagag actaaacaaa tcgaatctga
    11041 tttgaaggtg catcaaagcc aattgcaaaa tcaactgcaa tcaaaaatgg cgaactatca
    11101 aaagatgatt caggaatacc aggccagtgc ggctacaatg actgaggtta tcagggcaga
    11161 taaagaaaga gaaattactc agcttgaaga gaacattcag aaatttcagc aagacgccca
    11221 ggtttcattc cagaaaaaac agtccgatct tttgcaaccg gtttatgaaa aaatcgggaa
    11281 agctatcgaa gtggtagcaa acgaaggaag tttcacctat gtcctttcaa ccagaatcgg
    11341 ctctatagat gtgatacttt ttgcaattcc tgagaatgat atctcggatc tggtgctgaa
    11401 aaagatggga attactccac aaaataacta gataacaaag aactcaaagg tgaaagctcc
    11461 ttaattacga ggagcttttt ttaggtcgat gataattcct ccgtttcttt ctccgggtga
    11521 ccaggtcggt atttctgcta ccgccaaaaa ggttgaccag aagtatttgt ctgaaggcat
    11581 cgaaatttta aattcctggg ggctgaacgt aaaaacggga attaatgtct taaaagaaga
    11641 aacgtttttt gccggcaatg aagatgaacg cgcgtcggac ctccaggact ttatcagtaa
    11701 tcccgggata aaagccatct tttgcgcaag aggtggatat ggaacaggaa gaatacttga
    11761 caaaattaac ttcagtccgc tgcttagttc acccaaatgg atcattgggt ttagtgatat
    11821 aactatactt cacctgaaat taaaccaatt aggtcttgca agtttacatg gaccaatgcc
    11881 cgcaattttt cataaaacga ctgaaaatgc aattgagagc gtcaggcaat ttctttttgg
    11941 cgggggactc aaacctttat tcgcaaagta caataaggaa aaccgaatgg gcaaaacttc
    12001 agggaaagcc atcggcggca atttatccat gatctgccat tctctgggaa caagtttgga
    12061 aatagacaca aacaataaga tcctgatcct ggaagaagtt gatgagccac tgtataaaat
    12121 tgatcgaatg atgcatcaat taggaaaggc tggaaaattt gaaaaactca gggctctggt
    12181 catgggctgc tttaccatcc ctgaaaaaga atcaaattca tttgattact ctcttcatga
    12241 aatcattagt taccatgtta gatcgtataa ttaccctatt gcatccggtt tttcatttgg
    12301 gcatgaacct gaaaattatc cgattccact atcagttcaa ataaatattg atattggaag
    12361 ccagggatca acaatttcat actaaaagag aaatccggca attagtcctg agataataat
    12421 ataaggtgcc ggaattttag tataccggag gataagaaat gtcagcaata caaggccgag
    12481 ataaaccggg ctattccaca ggagtaagaa aagttgactc aggacttcaa cccagtcggg
    12541 agtagtaaag gccgcattaa aaggttgaaa aaaaagaatt aacgcggcag aaatcaccat
    12601 accggcacta gccgcattta ttccttcaag gctggcacgc acgacgcgat attttttcag
    12661 gtcttcccaa aaacgtatca caaagaaaat caggaatgta cccggaagaa aaatgccgaa
    12721 tgcagccatt aggccgccaa atatttcccc acctacgcca agatcgcgca tggagagggt
    12781 tccaacgaaa gcactaaagc taaaaaccgg accaggaatt gcctgagcta tggcatagcc
    12841 actcagaaat tcctgggctg tcaggtaatg cttaaactgc acaaattcgg tgtacaagta
    12901 gggtattagt acctgtcctc caccgaaaat caaactgccg ttacggtaaa aattttcaaa
    12961 caacaaaata ctccgttccc tggtaattcc accaagtagg gccgcgaata aaagaacgcc
    13021 gccccataaa agaaaattcg cccattgaat ccggactgga ctcttttctt caatgggatg
    13081 tttccggtat ttgaaaccag ttataaaacc acccattaac aacaggattg gaaaaatgta
    13141 aggcttcctt aaagaaaatg aaattatggc ggagataacg cacaaaataa ccgcagtatt
    13201 ggtgattaca actttccggg aaataacgaa agccgcataa ctgacaaaac ccaccgccat
    13261 aggttgaatg aaccgtgcaa attccagtga ggcgttcttt tcaacatagt gattcaggaa
    13321 aattcctgcc agcagcataa ctgtcacggc cggagttatc cagacaagca gggttaaata
    13381 agccagattt ggaccgccaa tgcgaaagcc aatggaagtt acagtttggg tggatgtagg
    13441 accgggaaga atattacaca aagcgaataa ttcaaccagc tcttcttcat tcaggtaccc
    13501 ccggagctta acgaatttgt caagaaagaa ggcaaaatgg gcttgagggc ctccaaatga
    13561 agtgagagat agaataagga catctctcag aaagatgtag tgtctgactt ttttcaatcc
    13621 aggttcgttt tgcgagatta tttttttaag ccaatttctg ctaaacgttc attcaggaat
    13681 tctccagcgg taatatcttg aaaaattctc ggattttcat catcgatgca attttcaagg
    13741 caggataaat ccatttctga gcggggatgc ataaaaaacg ggatcgaata tctggaggtg
    13801 ctcatttttt cccttgacgg attaaccacc ctgtgaatcg ttgatattaa ctttttgttt
    13861 gtgagtcttt ctaacatatc acccacattt acaacgatct ggtttgggag tgccgttatt
    13921 ggaatccaat gaccatctcg tctcagcact tgcagcccgt cggcgctggc acccatcaac
    13981 aaagttataa gattgatatc tccatgctcg gcagcgcgta ctgcatccgc gggaactgca
    14041 tcaggatttt ctatgggata ataatggata ggtcgcaaaa tggaatttcc attctttact
    14101 ttattgtcaa aataattttc ttcaagaccg agatacaatg caattgcatg cagcatttgg
    14161 atcccggcct gctccagttt tcgatacact tctaatccga ttttacggaa ttgcgggagt
    14221 tcatcaaccc agatgttatc agaatactcc gctttaatgg gatcatcgcc ctctacttct
    14281 tgtccgatat gatagaactc tttaaggtca cccgttgacc ttccctttgc atgttcttta
    14341 ccctttccca catatccgcg ctgaccagaa agttcaggaa tttcgtattt tcttttcgtt
    14401 tcatccggaa ggttgaaaaa ttgctttaca atggcataca attccgcggt aagttcatcg
    14461 cttaccccat gattcttaac ggcgacaaat ccaatatttt ggtaagctct tcctaaatca
    14521 ttgacaaaat tctgtttcct cgccttattg ctgcctgtaa aatcagataa atccaaagat
    14581 ggtatctcat catagaaaac aggttgcata tgtttattga ttattgtctt tcaccacaac
    14641 attaattgtg ctgctcaaag gcttgccata agattggtga aatccgtcgg caaactgcaa
    14701 ggtaagagta taattacctg gagccagttc aaccgtatcc ctgatttgtc cgagtccgta
    14761 atgaatatga acagaatctg ttggaacaac tacaccttcc tgtatatggg agccgtttat
    14821 caccaggtga tgatggccca taccctcttt aacctcaccg gcaggatcaa tttccattcc
    14881 ctctaaaccc atgtcaatca tgagcgggct acttaccgta tcaccatcat ttggtgagat
    14941 aaagtatacc tttgctgatg tcatttcagt tgacacagaa tcggtctcgc tactttcctg
    15001 ttcgctgctt ccagttcctg agcatgatat gatccaaaaa gttacggtga atgtaaataa
    15061 gataaatgtg agctttttca tagttgtcgg atttttcaga attatactaa attaattttt
    15121 tggttagaat cagtccagaa tttcctttaa aacaccaact gattttattt cgccaagatt
    15181 atctatatgt cccctataat aggcaattat caaatttaga cagtttcttc gagtcgaatt
    15241 agaggaaaca gaaatttttt gagttttatt ctcaatcatt tgtttaatta ataataatgc
    15301 atcgcggcct gaagcgtgca aatttgaggg aatatgattt ttgaaatcaa taatagactg
    15361 gggtttaaat ccgaggatgg cggaaaactc aatcaggaat cttaaatgaa aagaactttg
    15421 attttcattt tcagtatcaa actttattag ggattgttgt aggaaggcga aaacttcctc
    15481 aggattagag cagtcgcgca acgtcttctc aagcacttca gtaagaaaaa taaatatcac
    15541 tgattttttg atgttgcctg atatggtgga atacggatag ctgcatttca cttctgataa
    15601 tctttgaatg cctgcattct ctttgtagta aacaacgagg tcaagcaaag tcagtggctg
    15661 gaataaagcg attttgaatt tacctttagg tgaccggatt ccattaatca ggtaactctg
    15721 aagcccaaat ttttctgtga atatcctggc gataattgac gtttcacgaa atttgatttt
    15781 ccctaatgta ataccgctgg ttttgctaat cattttatct tcacttatta ctgctcatcc
    15841 cctcaagaaa acattttctg caccgtgctt cataatgacc tttttcccca agcacaactt
    15901 tctcattaag tccggataac cggaatgaat aggaggcaag acttccacac ctggcacaaa
    15961 ttgcatgcaa ctttgttacg tattcagcga cggacatgat ggatggcaga ggaccaaagg
    16021 gtttgccttc gaaatccatg tcaagaccgg caattattac acgcttgccg ccattggcca
    16081 gttcaacaat gacttctgtc aacgaatcat caaagaactg agcttcatct atgcctacca
    16141 catcgcactc acctgtcaac agcaaaatat ctgatgggaa atttacagga gttgaccgga
    16201 tagctctttc atcatgagaa acaacatctt cttcatgata tcgcttatct atggctggtt
    16261 tgaaaatctc aactttctgt tttgctatca aggccctgtt taacctccgg attaattctt
    16321 cagtttttcc tgaaaacata gaaccgcata tcacctcaat ccagccgccg cgggtattcc
    16381 ccggatttga tctacctgaa tgaggttcta taaacattta acttaagaat actttaaatg
    16441 tggcaattga ttgtctttgg gtaccaaaat agttaaatct cctgaaggta tggcctggca
    16501 agctaacctt tcattttggt tgagttgtct caatcttttg taattttttt ctgctgttgt
    16561 tagcggattg agattttgcg aaccactaac taccaccatt ttgcaggttg tgcatcttcc
    16621 cttaccccca caggagtgca gccagtcaac atagctttgc agcagaattt taagcacatt
    16681 tttacctgac ggtttaaaga ttatctcctt ttgatagaag ttttgtataa tgattcgcgc
    16741 catattgtat aattttacaa aggaaaaaat aaatagcatg gagaacaagt tgaatctgga
    16801 gtcgaccatt aagtattccg aggagttctc aaaaattttg tgcgacaggt ttttcagcac
    16861 ggaaaagcgg attacgggtg aacagatatt gaatctgacg aacatcagac aaatcaactt
    16921 atttgtaata agaattctat tctcaaaatg gcaaaaggag caagggaaga taaagagttc
    16981 atattttgat tatacaaatc atgaaattca aaaagcatta aaagacttcc tgaacaagtt
    17041 gtcgcaacac atttctgtgg cgaaggaaga tttttatccg ctgttgaaac ttgcctgcat
    17101 caaaacaata caggtaattt atgctcccta cgattttttt atgcaggaaa tttccggcga
    17161 cagtgatgat aatattaatg ttgaaaagct caaagaaagt tctaagtatt taaagataaa
    17221 taatggattt ttcaatgagc ttcttcaaag gcttaaccaa tcaggagcga aagagttaag
    17281 ttcaacggaa gcccgcggaa ttttggaaga agttttcctt aattattccg gttcaccgga
    17341 agatggtgag gcttaccttg cgcatttcaa tgaagttctg gagctcagag tggaagattt
    17401 atttactccg gaaaaagaag ttaaacccgc agagaatgaa acttcttatt caacaataaa
    17461 cgatgaccat gcaaaccgca ttggagaaac aatcggggag gcattaaaga agattagtaa
    17521 gacaaatctt caggagagtg tttcagtcaa ccaaaaattc atgtttgtgc atgtgttgtt
    17581 taacggtaat ccaaaattat ttgaggaagc cttgggaaag attcaggaat ttgattccaa
    17641 agaacaggca atgcaattcc tggaaaagga actaaagaac acatatcaat gggacaagga
    17701 atcagaggag gtgggggaat tttacgatct ggttcttacc aggtttgatt aatgcacgag
    17761 ttgatccatc ctgtgggcgt ccagcttaat caggacaaaa agtaaacatg taaaagccca
    17821 caaggaagag ccgccatagc taaagaaagg taacgggatt ccgattaccg gaaatagccc
    17881 gatggtcatc ccaatattaa tagcaaaatg aaagaacaaa attgccacca cagaataccc
    17941 ataaacccgg gcaaaccttg ttttctgtct ttcagaaata tatactattc ttaaaaagag
    18001 caacataaaa agtgtgataa gaatcagagt gccgaaccat ccatgctctt caccaattgt
    18061 gcaaaagatg aaatcagtgc tttgttccgg cacaaaatca aattttgtct gagttccttc
    18121 caggaatcct ttaccccaca tgcctccgga gccaatggct atttttgatt gagtcacatt
    18181 ccagccatac cccaatggat cctcctccgg attgattaat gccttgattc tgttttgctg
    18241 atgaggctta aggacattgg taaaaacata atcaacactt cgtataagac tgaagacaat
    18301 tgctgctcca agaagtattc cggttatagt ttttagattt ttttttgaaa gcattattat
    18361 caaaaaagcc gaagcgccaa taccaatggc cagatagctt tgagttacca gaagggccaa
    18421 caggaaaata gcagctgagg cagcaacaaa aatcaggaat aacggagata acccttcacg
    18481 gtaaaatacc aggatgaagg ctccaaaaat aagagcggta ccggtgtctc cctgtaatac
    18541 gattagcccc attggcaagg cgatgaaggc acctaaaatg aaaaggtctt taaagttgtt
    18601 gaatttaact ccatgactgt ccataaattt tgccacagcc agcgcggcgg caaatttcat
    18661 aaactccgaa ggctggacgc caaaactccc taatcggatc catgatttcg atccagcgat
    18721 ttcagttccg aatcccagca cacccagaag gacaacgaaa acggcgccat atataaaata
    18781 agcaaaagtg gaataaaact taaaatcaat tattagaata aaaattataa tgatgacgga
    18841 ggctccaatc cagaccagct gccttcccga gttcagggag aagtcgaaaa tacttccggt
    18901 ggtgccctca tcgtaaacgg cagcataaat attcagccat cccaacagaa caatgagaaa
    18961 gtaaagcaga attgttaacc aatcaatgga actaactaaa ttttcactgc gttgcatctg
    19021 caaaatctcc ttttaataca taatcttcaa gccactgccg ggtaatatta ccccggatgt
    19081 aggtttcaat caacaaactg gcaatagaag cggcagctct gcctccccat ccggaatttt
    19141 caacgtagac ggaaattgca atttgtggat ttttttttgg agcaaaagcc atgaacactg
    19201 aatggtcttc accgtgtgga ttttccgcgg ttccggtctt tccgcagatc tcaatatctc
    19261 tgattacggc tctccaggca gttgaagtaa ctacatcctg catggcgtta attacgggag
    19321 gaaaatgttt ttcgtcaatt cccgccatat gtttttctgt aaattttgac aatggcccgt
    19381 agtcaccaat ctcctttacc aggtgtggag tgtaataata cccacggtta gccattatgg
    19441 cggcaaggtt tgccatttgc agcgggctta aaagcatttc cccctgtccg atactcaaag
    19501 aactgatggt gctgaatttc caactgttat gtccataaat tttatcatag taatccgagc
    19561 cgggaataaa accacctttt tcattcggga tatcaatgcc aagcggagcg ccgaagccaa
    19621 agctcataac atgactcttc cagttatcca ggccaagcct tgaatcaatg aaggtatttg
    19681 cattgagatt ttgattaatc attcttctga aagccatgaa aaaataattg ttggaagact
    19741 tcctgattgc ttcgtgaagg gtatagtaac ccgggggcgc gtggtcacca atgtttcttc
    19801 catcaatata aattgtttcg ttaacattaa gcacattttc ctgaagcccg attaatgatt
    19861 gtacgatttt gaaaattgaa cctggcggat acatggccat tacaggtctg ttgaaaagtg
    19921 ggttgaggga gtctcggcta attaagctaa agtttgaact atattttcgc cctaccatca
    19981 tacccggatc ataggacgga cctgaaacta tggcgagaat ttctcccgtg gctggctcaa
    20041 tagcgacaac actaccaact tttcctttca acaacttctc agcataaagc tgtaaccgca
    20101 aatgaattgt tgaaactaaa ttttgacctg gaattggaat tgtatcgaat tcaccatctt
    20161 cgaaacttcc tttctctatg ccccgaacat tttttatctt atagctaatc ccccttttac
    20221 ccctaagttg tgactcataa tataattcca gtccgctttt gccaatgtag tcgccttgtc
    20281 tgtagtagcg ggaactgtct tgccgcaatc ctgcactgct gatttccgcg atgtacccta
    20341 atgaattaga taataccgga tgtgcatatg cccgtgaagt ccgtgcctga attccaaacc
    20401 cgggataatc aacaagttta tcctggattt taccaaactc ctgattagag atcatcttaa
    20461 aaaaaggaga tggttttaca taactatact ttcttgccgc ggaaagtctt tgcttgaagt
    20521 cttcgcgcgt gaggcccagt atctcgcaaa tagataaggt atcaagttgt ttaacctctt
    20581 gtgggatcac ggtaagatca aattgaggat tgttgtaaac aatcaattca ccatttctgt
    20641 cgaaaatcag tccgcgatac ggatattcaa caacaggaag aataatatta tcagtagctg
    20701 cttttgcgta agtcttatca gtaacttgta tggtaaacaa cttcaccagg aaagcaattc
    20761 ccgtaattaa gaaaactgcc tgaattacat atttcctgcc ttcaatcatg cccgattttt
    20821 tctaacaaag aataaatact gataaagtat gaccgtaaag taagtgaaaa ccattgaagc
    20881 caatacctta ctcaaggtaa agaagaataa ccccttgaat ccacctgctt caatgtaaaa
    20941 taaaagaaaa tggtgaataa aaatcaaggg aagagcataa atagtaaacc attgaagtcc
    21001 gagtgaggca attgttggag tagagccaat ttcgtatccc cctcgtggga ttatagattt
    21061 aatccaaaat tgcctggcga acatcatcaa aacgcaagca gcggtgtgga cacccaggct
    21121 atcgtaaaag atatcaatta caaatccggt aaaaaaacca gccagcaaca ggtagacagc
    21181 cgtggtttca aacgaaagga acaaaaggaa agccacatac aaaaatgcga aagagacacc
    21241 aaacagcact gtatttttaa atatcagcgt ttgaacaagt ataaatacta taaaattcag
    21301 catggttaat atcaagcttc tggtatccat taaaagcgtt cgttaacacc aagtgagtct
    21361 aactcatccc tgattgcgct tataataact tgtacgaatg agagattagc aaattctgtg
    21421 gagagcttta tttcgatttc atgaaaaggg gcaccttctc ttaaactaat tttagaaact
    21481 gttccaataa gtatatcttc cggaaagata gaattaaatc ctgaggctga caccgtatcc
    21541 ccttccagca gcacgatatg cgtgggaaca aaaagaagct gagcaaaact gggacttact
    21601 ccatcccatt gggtggaaca aagattgatt ttgccttcta ccttcgagga gataagaaaa
    21661 tttgaatgaa gaagggaggt aacaaccgca aaattttcgc tggtgtattt tatcttaccc
    21721 acaattccgt taggaccaat aacagccata tcgggtttta caccttctgc attgccccgg
    21781 tttattgtaa gatagttatt gaaattatac gcggagttac tgattacttt cgccgtaacg
    21841 aattcataag gaatgtacgc tgagtcctgc acgattccct taaccggagc aacctttttt
    21901 cgctccaatt cttttcgaag atttgcaatt tcttcagcaa gttgtgtatt ggttttcttt
    21961 aagctgaagt agcctcgaat ttcggaagaa atggaaagca aattagcgga gatggcattg
    22021 gaagaattaa agaaagcagc gctttgatat ctattgtttt gaacaataag ccagaagctc
    22081 agaacctcaa gaaagataaa aatgagggtg actctgtatt tgaatagaaa tagatataat
    22141 cgatacattt atccattagg tcatcagtac gcctatatat tggtcaagac ctttcaaggc
    22201 cattcctgtt cctctaacca ctgctctcaa tggatcatcc gcaacgtgaa tgggaagttt
    22261 tgttcttaaa gccagccttt tgtcaagtcc gcgtaacaac gcgccacctc ctgtgagatg
    22321 aattccattg tcatatatat cggcggatag ttcaggcggt gaaatttcca gcgctttaag
    22381 aacagcttct tcaatttttg atactgactt atccaatgca aaagcaattt cagaataaga
    22441 aacttttata accttcggaa ttccggtcat taaatctcta cctctgattt cataatcatc
    22501 cggtccatca tccagttcgg taagagcgga gccaacctca atctttactt tttctgccga
    22561 tctttcacca atcagaaggt tatgctgccg tctcatataa tccagaatgt cccggttgaa
    22621 tgtgtcgccg gccaccctga tggactgatc acacacaatt cccgaaagag caatcactgc
    22681 aatttcagta gttccgccac cgatatccac tatcatagag ccaatcggct gttcaatgtc
    22741 aatcccaatt ccaatggcgg cggcaatcgg ttcatgaatc atatacacct ctttggcacc
    22801 tgcatgctcg gccgagtctc ttaccgcgcg cttttccact tctgttatgc ctgagggaat
    22861 gcagataatc attctgtgag aagacggaaa ccagcggtta cccatattaa tcattttgat
    22921 catccccctt atcatgtgct ccgctgcatg gaagtcagca ataacgccgt ccttgagagg
    22981 tctgatggtc tttatgtttt cgtgtgtttt ttcatgcatt tgcatggctt ctcttccgat
    23041 ggccagaact ttgttattag ttttatttat ggctatgata gaaggttcat caactacaat
    23101 tttgtcctta aaaatgatta aggtattagc agttccaagg tcaatggcaa tgtcgctaga
    23161 gaagaaatca aataatccca ttggttagat attagtcgta aaatttatgc tgattcagaa
    23221 ggcgctcaat ttacaaaatt gatacaaata tctatttaaa tcgctaaaaa tctaatgttt
    23281 gaagtgtctt attccggtga aggccattgt cattccatgc gagttacaaa agttgatcga
    23341 atcctgatct ttgatgcttc cacccggctg aattacggcg atgatcccgg cattgtgagc
    23401 aatctctaca cagtcgggaa aaggaaagaa tgcatcggaa gccatcactg cttttttaag
    23461 atcaaatttg ttggcaccgg cttttgttat tgcctgctgc agcgcgtcaa ctctggaagt
    23521 ttggccaaca ccgctggcca agagctggcc atccaccgct aatacaatgg tattggattt
    23581 cgtgtgcttg gcaattttag aagcgaatag catcccggca atttgttcct tgttgggatg
    23641 aacttttgta actactttca ggtcctcaac agaatccgtt tttaaatccc ggtcctgctc
    23701 aattactcca ttcaccaaat tcctgaattg ctttgagcca gtgatcttac ttttttgttt
    23761 caggagaatt ctgtttttct ttgattgaag tatttgaagt gacttcgcgg aatatgcggg
    23821 cgcaatgaga atctcaaaaa ataaatcgtt aattaacgag gccgccgatt catcaagagt
    23881 tttattcgtt gctaaaattc cgccgaacgc ggaaacagga tcgcccgcaa gtgctttttg
    23941 gtaggcttcc gcaactgagg cagccgttgc caaaccacat gcatttgtat gtttaataat
    24001 tatgaagaca gtttcatttt caaactcttc caaaaggtta agagcggagt caatgtcaac
    24061 caggttatta taggaaagct cctttccgtg aagttgttcc agagtatcat tcaggctgcc
    24121 atagaaaatt ccctgctggt gtggattctc tccatatctt aaggctttgg actcacgtaa
    24181 gctttgtttg aaggaacgaa caccttcttc tctgttgaaa tagttaaaaa tcgcggtgtc
    24241 ataatgggaa gttacctcaa acccacgggt ggcaaatagt tttctctgct caggggtagt
    24301 ttctcccttc gataaattca aaatgtccag caattctttg tattgttccc ttgatgatat
    24361 aattaatgta tcctggtaat tcttagcggc tgcacgaatt aacgagattc caccgatgtc
    24421 aatcttctca attatttctt tgtgcgatgc gccagctgcc attgtttctt caaatggata
    24481 taaatcaacc aggatcaaat caagggcggg aatctcatag tttgaaagtt cattttgatc
    24541 agattcattt tcccgccggg aaaggatacc gccaaatatt tttgggtgga gagtttttac
    24601 ccgacctcct aaaattgatg gatacccggt taagctttcc acggatttaa ccggaacatg
    24661 aagttgttca ataaaggttt gtgtaccgcc ggtagaatag atctcaacct ccagttcgtg
    24721 aagccttagg acaatgcttt caaggttatc tttatggaaa acagagatga gtgcggtatt
    24781 aattttcctg atggtcatta tgcttaacag actggaggtg caaaagtaat ttttttcaac
    24841 ctaatttgtt tagcacctct tcaattacct tcggataata ttcgtattcc agttgatgag
    24901 ccttatgggc tatcgatttc ggattttccc cggctcgtat tgagcaggaa gtctgaaaaa
    24961 tgatatctcc ttcattataa tgttcattca catagtgaat ggttataccg gattccgtat
    25021 ctcctgattc tgagacagcc tgatgaaccc tggctccata cattcccttt ccatcatata
    25081 aaaaacttca atgtttggta atgaggtggg ggacagaata atttttctgc ggaaactaga
    25141 agccggggca gcgaacacag tttcggaatt catgtggcgc agatggccgg catgccaaat
    25201 tccattgtat tgcgaaacga tgaggtacta cattttcctg gaaaaggata agagaaaaac
    25261 cagggtaaat aaatgaagga agtgcccaaa tcagatttcc agtttacact ttttgaaacg
    25321 atctgcggtt taatcaggtg aaagaaatgc tggttccgaa tggatatcaa caacgatgtc
    25381 accgatggga gcaatgctga agctgaatga gataaaggga ttgctgaaaa tcgtagtggc
    25441 cacagaaaat ttccggcaat catctcgggg ttcaattgca tcaaaataaa actacacacg
    25501 caaccaacct tctgccgaaa atttgcgtgt tgcttaaaaa gacggtaatt taagcaacgt
    25561 ctctaaagac catagaagga aaagcattat ataaactcct ggattcctgc cgggggggaa
    25621 acagaaacag ccaggatcag ttgtacaggg aattttatcc ttatgcaata ggtatatgcc
    25681 tgcgttattc ccgcacccgg gatgaagcca tagaaatttt aaacgacggg tttttgaaga
    25741 tttttacaaa acttgatcac tatacaaaag gattgtcatt taccggctgg ctgagaaaag
    25801 taatgataaa ttcggcgatt gattattacc gaagaaatga aaaacattat aacgcacttg
    25861 atatttcgca tgttcaatat gaagccgtaa gtgaaacagc attggatgct ctttccgcag
    25921 aagaaataat tgcagccgtg caaaaacttc cgccttcata caggatggtt tttaaccttt
    25981 ttgtgattga ggggtacaaa cacgttgaga ttgcaaatat gattggaata agtgtcggca
    26041 cctccaaatc aaacctggcc gtagccaggg aaaaactgaa gaaaatgttg atcatcgaaa
    26101 atgataaatt aggcaaagaa aaaaatggat gacaggaaat ttgatgaatt gttaaaatcg
    26161 aagatcggtg aataccggaa tcccgggttc gagccttctg ctttggctgc acttcatcaa
    26221 aaaatggccg ctatttctca accctggtat gggcggtata gaaccgaatt gatcgccgga
    26281 accgccacct tactctgcgc gggcatgata ttccttggac aatattttag taaccgggta
    26341 aatgaacaat ggatggccac caatatccaa gctttaaaag atcaggagaa ccaaattcaa
    26401 cttctgaaag aagaaattga acgattaaga aatctggccc cggatacaat acggataacg
    26461 gaatttagcg gtcaggataa ttccgggtat tacttgcttc tgttaagtaa aatatcagca
    26521 ctggaacgaa aacttgaaac tctgggaaac ccggagaatc agatgcgaaa ttctcagcta
    26581 attcccctgg cagattacaa cgcaggcaaa ttctccaact ctcttatatt tgataagaat
    26641 gaatccatga gaattcattc tatatcaagg ttgaatacgc gaaatccgga agtgagcaga
    26701 gcggaaggaa aaattgaagt gtctccaaag gaaaaagaaa gcgtgaacct ttctgcaaaa
    26761 acaatccggg acatcgaaaa acattatatg cggggagttg gaatccgggt tggcccggct
    26821 gttgaaatcc accgcggttt ttattcggaa ggggaaggag cgtacaattt tggaggtgga
    26881 attgccgccg attttattat gtcaccgtcc ctttcgctgg aaactggagt taagtatgcg
    26941 ctccatacta acaaagttaa aaatgcagat tcatctaacc ttaattttcc ggatgttgac
    27001 ccccagttgg gccagttaaa tacagctgaa attgattttt attctcttga actacctctt
    27061 tacctgaaat accggtatcc gtttagtgac aaaacctatc ttcaaaccgg agtaggatat
    27121 tcattcttca tttacacgaa agaagaattt gattacgatt atgaaataag cggaaacccc
    27181 ggagccagca tcaataccct cgttaaagac aagaatttta cgatttaccc gggtacgcta
    27241 aatttttccc tgggactttc caaggagtta aaaaacaaaa aaattctgga aacttcactt
    27301 ttctatcagc atggagttgg atccatggga attgaaaaaa taaaacttca gtttttaggt
    27361 gtgaaaggtt cctactggtt caagataaga tagaaccggt tcaaaaaaca cacctactgg
    27421 aaagttcaga atgagggaaa atttcaaacc ctcaaccaac caaaacagaa atccggccgt
    27481 gatgtcagta actaaccttt tcaaaaaatg aagacccgat tatcgcgact gttaacattc
    27541 ggtctgatag tatttatgac tttgaatgtg ttttcctgcg gggaagataa tcccctgccg
    27601 atctttgaag atgatatttt gctggttgca gcaaatctta atcccgcaga tttcgcttca
    27661 gaaatcaata acccgtattt cactctgttg accggtgata ttcacatata tgaaggagtt
    27721 gatgaagatg gagtggaaga aagggtggta tcccgggtta cattcgaaac gaagacaatt
    27781 ttgggagtaa actgtgttgt agtacttgta aaggagtaca gcgatggcag tttaattgaa
    27841 gaaacccgcg actggtacgc acaggataaa aaaggaaatg tctggtattt cggagaagat
    27901 gtaaaagata ttgaagatgg ccaggtgtac agcactttcg gttcatggga agccggtgtt
    27961 aatggcgcaa agccaggcat tatcatgttg gccaatccgc agccggggcg gaaatatcat
    28021 caggaagtgg cagttggaat tgccgaagac caggccgaag tggtgagcct gaacaacacg
    28081 gtgaccgttc cttatggtac ttttcaaaat tgccttaaaa caaaagaaac gaatccgttg
    28141 gatcctggtt ttgaagaatt taaatattac gctccggatg tgggatttat taaagcagag
    28201 cccgcagccg gcggcgtgga agaacttgcc gaaatcacgg acatggtaga tcgggcttta
    28261 ttaaacgccg gcaatattaa tccggtgatg tttgttgaag gtgtggataa cccttacttc
    28321 ccgctgactc caggaaaggt ttacacgtat tcagggttga atgacgaaga tctgccagtt
    28381 acggtaatat cagaagtgac cacggaaacc aaagtaattt tgggagtggt atgcacagtg
    28441 gttcacgaga aggaatttga agacggtgaa ttgattgaag atacctacga ctggtatgct
    28501 caggatgtgg agggtaacgt ctggtatttc ggcgaagaca cgaaatccat tgaagatggc
    28561 gtggtggttg gaaccggcgg ttcctgggaa gcgggagtta atgaagcggt tcccgggata
    28621 attatgctgg caaacccaat ccccggcatg aaatacaggc aggaagttta tgaaggagaa
    28681 gcggaagatt gggcggaggt atcaagtatc aataacaccg ttaccgttcc gttcggcacc
    28741 tttgaaaatt gtattaaaat tttaacatct acaccgcttg aagcagaagt gctggaagtc
    28801 aagtattatg ctccgggtat aggatttatt aaaggtgaag atcaaacgtc ttcggagttc
    28861 gaagcattgg aaaatatttc aaattgatag ttcaaaccaa cccttttatg aaacccagcg
    28921 tgttgtcagt atctgaccgg ctgaaaataa aattatatgc ggatgaaagg atttaaaccc
    28981 aatcacttcc tggcgtttcc cctgattttg atgattttga attctggcgc atacagtcag
    29041 gagaagttta ccctcagtgg aattatcaca gatgaagaaa ccggcgaagc gttaatcgga
    29101 gctaatattt atgtcactac cctggaaaaa ggaacctcca ctaacactta cggtttttat
    29161 tctcttacct tacccagaac cgacagtttg ggaatcatat tctcctacat cggatatatt
    29221 ccccaaatta aaaaaatcta tgcggataaa aacctggaac tggatataga aatgaaaccc
    29281 tccacaagca cgctggagga agtgactatt caggggcagc aggatgcaaa cgtggcgagg
    29341 ccgcaaatga gcgtggtaaa tgttcccctg gaaaaaataa aagagttacc ggtaattctg
    29401 ggagaaatag atgtattgaa agttattcaa ttgctgccgg gtgtgcaatc aggtaatgaa
    29461 ggcacaaccg gaatttttgt cagaggcggc aacgccgatc aaaacctggt tcttctggat
    29521 gaagcaaccg tatataatcc cagtcacttg ttcggattgt tcagtacatt caattccagg
    29581 gcattaaata acgttacttt gataaagggc ggcttcccgg cacaatacgg aggccggctt
    29641 tcttccattc ttgacatttc tatgaaagaa ggaaataacc gcaaatttca gggtgaagga
    29701 ggaattggat tagttacttc gcaactaacc ctggagggtc ccataaaaaa agagaaagct
    29761 tcattcattg tctcaggccg gcgtacctac attgatttga ttttaactcc tttcctcgaa
    29821 gttggaaaca gttccgatta tcatttctat gatatcaatg ggaaagtaaa ttggaaaatc
    29881 tcttccaaag accggctttt ctttagtttt ttcaccggaa aggatgatgc attgtataat
    29941 gaaacaaaag gaatcagtta caacataaaa ttcggcaact cggccgctac cttacgatgg
    30001 aatcatttgt tcgggcaaaa actctttctc aacacttcac tgatttacaa tacatatgaa
    30061 caggacattg ccaccatcca ggataatttc ttttcacagg tggtttccgg aatcaatgac
    30121 gttaatggaa agatggagtt tcagtatttc ccttccctga accatgaaat tcgttttggt
    30181 gcccattaca ctaatcacag gtttttatcc ggcggaaaat cagaagcgca aaccagtgga
    30241 aaccagacca tcaatgttag taaccttcct tcaaaatatt ttagtgaata tgcattctat
    30301 gtgaatgatg aattcaatat ttaccgcggt ttctcggcca acctgggttt tcgatttccg
    30361 ggtttcagcg acagtgatgt aaattatttt gaatgggagc cccgggcaac cctaaagctg
    30421 cagttagggc caacttcatc cgttaaagcc gcctacacgg tgatgcacca atttcttcac
    30481 ctggtgccca gctctaccgc ttcggtaccc accgatatct ggctgccttc ttcaaaaaga
    30541 acaaaaccgc agctttccga acaattcgca atcgggtatt tcagaaactt taaccaaaac
    30601 cagtttgaaa ccagtgcgga actttactat aaaacaatgg ataaccaggt gttattcagg
    30661 gaaggaaacc agttaatcga aagccttgaa gttgatactg ccctggttta cggaaaagga
    30721 gaaagctatg ggctggagtt ttttctgcgg aaaaataccg gcccgcttac cggatggctg
    30781 tcgtacactc tttcaaaaac cgatcaaaaa ttcgatagct tgaattttgg cagaactttt
    30841 ccatttcaat atgacagaag gcatgcagtt tcattagcca ttgcttataa gattaacccg
    30901 cggtggacag tttcagccgt ttttgaatac gcaacgggaa aggttaaaac cctgccggtg
    30961 ggcagaatta attcttacta cggcggaagt ttgtatgaag gaacttttta cgtgtatgag
    31021 gaaagaaata atgcgcgcct gaaagattac caccggctgg atatttcagc cacctataaa
    31081 agaaacagga aaatttttag aaaggaatat gttgccgagc tcgtatttgg agtgtacaat
    31141 atttacagca ggcagaatcc gtactttgtt tattttttgg tagatccggt taccgatcaa
    31201 cccaaagcaa aacaagtctc gctattacca attctaccca gcctatccta taatttcaaa
    31261 ttctgaaaaa catgaaaccg caaaaacttt cttctatatt tatacttatg ctggtcgtgg
    31321 ccgcgtgcga aagagaaatc aatattaatc catccacggg gtttaccggc gatatattta
    31381 ttgaaggtat tttatatccg ggtgaatttc cccgcgtttt cataagtcgc agcccgggat
    31441 tttttgagcc cgatgcaacg ccccaggaat tgtttggcag aggagctgta gtaacaatta
    31501 ccggaagcat gggccaggaa atattggctg ccgactctac cttcgataaa tttcgttgtc
    31561 gctgggtgcc attttatgca ggaaataccc cagccattta tggtgaagca tatgacttaa
    31621 ccgttatcta tcaggggaaa tcgtattccg cttccaccac aattaatcaa cccaaaccca
    31681 tcattgatac ggtggaatac gtgggcgaat tttttgatat ctatggcggt cacgatggag
    31741 ttatcatcac attcaccgac agccccggtt ccggtgattt ttaccggtac cagatgaaca
    31801 ggatgataga caggaacact catcatgtgg atgtgctgga agtaattgtg aacgattgtg
    31861 ttgctgaagg cgaattattc cgggtaacag atttgggaag aagtgtgttt a
//
LOCUS       DASWHX010000112        25761 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_2692574, whole genome shotgun sequence.
ACCESSION   DASWHX010000112 DASWHX010000000
VERSION     DASWHX010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 25761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25761
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2692574"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..449)
                     /locus_tag="VGA21_07820"
     CDS             complement(<1..449)
                     /locus_tag="VGA21_07820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650452.1"
                     /translation="MKYRAFLCAILFFGIRAFAQQTPVQVSAFLNPPYSLALYEYGMP
                     GSTLLQVQLLLTDLTKNQYPVKLRLIVEGVGITIKTKATFLPQPIPLSGGTPYILTAE
                     DLQPYFSLENLDLLGISKRDYQRLGMLPEGFYRFTIQVLDYYRSSVV"
     gene            complement(673..1569)
                     /locus_tag="VGA21_07825"
     CDS             complement(673..1569)
                     /locus_tag="VGA21_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009053298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX9650453.1"
                     /translation="MINNAPSEKPGLKAWLLLILLSLMWGSSFILIKAGLRVFDPLEV
                     GSLRIAAAGVFLLPWAFKDIRKVNQREWKYLLYIGLMGSLIPAFLFAKAQTRIDSSLA
                     GVINALTPLFVLFIGAIFFARKIRRNQYIGLIIGFCGTALLVMAGSGGSWDQLNFYGL
                     FVVAATICYGFNLNIIKYKIESLKAISITSISISLVLIPATLFLLFGTDFISKMTTIP
                     DASLAFAAITLLGIMSTSIALVIFNGLVRMTNPIFTSSVTYLIPLVAVGWGVWDGEVL
                     LIGHFLGMIIVLAGVFIANAKK"
     gene            complement(1566..2558)
                     /gene="dusB"
                     /locus_tag="VGA21_07830"
     CDS             complement(1566..2558)
                     /gene="dusB"
                     /locus_tag="VGA21_07830"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009194110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEX9650454.1"
                     /translation="MVTLGNINLGDFPLLLAPMEDVSDPPFRAVCKENGADLMYTEFI
                     SAEGLIREADKSMQKLDIYDYERPIGIQIFGDKIESMRQAAEIAENARPELIDINYGC
                     PVKKVACKGAGAGILQDLPKMEAMTSEIVKQVSLPVTVKTRLGWDDTSIKIVEVALRL
                     QDAGIKALTIHGRTRQQMYKGAADWSYIANVKSHPDIIIPIFGNGDIDSPQKALDYKS
                     QYGVDGIMLGRASIGNPWIFNQIKHYIKTGEKLPDVGIKERVKVARKHLELSMEWKGE
                     KKGIHEMRRHYTSYFRGVDNFKPLRIRIVQSESFYEINSILEEIEGLYQESYAV"
     gene            2593..3558
                     /locus_tag="VGA21_07835"
     CDS             2593..3558
                     /locus_tag="VGA21_07835"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEX9650455.1"
                     /translation="MEHNGNELALQNSRKPALSFMLVLFFSFVGFTTLGPFLGILFAL
                     PFPGFDLNNLLSILENPVKFPESKTPFLIIQGISSVVGFIVLPSIFLVGIQKMGFRIF
                     FQAKTVTPVVIFVTILFIILFMVVNSVFVEWNANLKLPEFMSGFENWARGLEDNAAVL
                     TQFLTDFDNIGQFLIAFVVIAIIPAFGEEFVFRGLLQNYAIGFLRNPHVAIWVTAILF
                     SGFHLQFFGFVPRAFLGVAFGYLYFWSGNLTIPIIAHFINNGFTLLMIYMYRTDAIGF
                     DIETTESPGVLTVIPMAFITATLLYFFHKHTFQIRKQHGDMANRV"
     gene            3572..3742
                     /locus_tag="VGA21_07840"
     CDS             3572..3742
                     /locus_tag="VGA21_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017730263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650456.1"
                     /translation="MRAEIVKDVLLGRDITALIVNKRDSSYNTFGYFEVVVHNDNVIE
                     ALRIIEDEIVFE"
     gene            3723..4556
                     /locus_tag="VGA21_07845"
     CDS             3723..4556
                     /locus_tag="VGA21_07845"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009580084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEX9650457.1"
                     /translation="MRSFLSKYSNFTQRVVTGLTGAVILMTALSWSEWGYFICFLLVV
                     VISQLEFYKLVGLDGMSPLKFWGTFTGLSVFVLSFLIERGSVNTNFYYLIFPIACGTF
                     FIKLYKKSDKKPFTNIAFTLIGVIYIALPIALLNISTFVMGQYSHEIIIGLILIFWAS
                     DIGAYFAGIKFGKRKLFERISPKKSWEGALGGAAFALLVAYLISLYFTDLTFINWMIL
                     GAIIIVGGTYGDLIESLFKRSIEIKDSGTKLPGHGGFLDRFDSLLIASPFLVAYLKLF
                     L"
     gene            4641..5915
                     /locus_tag="VGA21_07850"
     CDS             4641..5915
                     /locus_tag="VGA21_07850"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010607949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HEX9650458.1"
                     /translation="MTYIEPAPIKDAENPFESMMSRFEIASRILGLDDEVYNVLKTPA
                     KQVVVSLPITMDDGSIKVFEGYRVIHSKILGPSKGGVRYDMAVNLDEVKALAAWMTWK
                     CAVVDIPYGGAKGGIKCDPRSMSAGEIERLTRAYTGALIEIFGPDRDIPAPDMGTGPR
                     EMAWLMDEFSRAQGMTVNAVVTGKPLVLGGSLGRVEATGRGVMVSALAAMEKLKINPY
                     QATCAVQGFGNVGSYASLLLEERGARVVAISDISGGYYNANGIAIRDAVDYRNQNNNS
                     LDGFKGGDFISNMDILLLDVDVIVPAAKEDVITIKNAEQIKAKLIVEGANGPTSAKAD
                     AIINEKGIMVAPDILANAGGVTVSYFEWVQNRLGFKWTSERVNRRSDRIMKDAFDRVY
                     KTSIDYSVSMRIAAYIVAIDKVAKTYKWRGGF"
     gene            5926..6585
                     /locus_tag="VGA21_07855"
     CDS             5926..6585
                     /locus_tag="VGA21_07855"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013454886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase family protein"
                     /protein_id="HEX9650459.1"
                     /translation="MKIHKEGKNLLFVLLLLLIAIDAVAYNFIVSQAALWIVIGFSII
                     IFLLVLQFFRNPTVVVPIDEKHIYAPADGKVVAIERVEANEFISIPSQQISIFMSPVN
                     VHVNRAPVSGKVKLFRYHKGRYLVAWHPKSSSENEQTTMIIQMSNGVEILVRQIAGAL
                     ARRIKWYVEEGDDVIQGNEFGFIKFGSRVDILIPEDFTITAQIGEKTKAGKTIIATVP
                     N"
     gene            complement(6582..7064)
                     /locus_tag="VGA21_07860"
     CDS             complement(6582..7064)
                     /locus_tag="VGA21_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020526664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX9650460.1"
                     /translation="MAEELIISKSRDKKKRYESLIPQIKALIDNEKNLIANLANTSAA
                     LKEAMNFFWVGFYLVEGDELILGPFQGPVACTRIQKGMGVCGTSWKNKAPIIVPDVDQ
                     FPGHISCSSLSKSEIVIPGIKDGEMILLLDVDSDKRSDFDLVDQKYLTDIVEQIIAKS
                     "
     gene            complement(7057..8085)
                     /gene="ribD"
                     /locus_tag="VGA21_07865"
     CDS             complement(7057..8085)
                     /gene="ribD"
                     /locus_tag="VGA21_07865"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009185295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEX9650461.1"
                     /translation="MEAERKFMERAMDLALRGLGKVSPNPMVGCVIAHKGEVVGEGWH
                     EYFGGPHAEVNAIESMKDKKILNRSKVYVTLEPCSHIGKTPPCTNVLIESGVKEVIIG
                     LKDPNVLVNGTGIRQLESSGIKVKLGYMEEQAEEMNRRFVTFHLEKRPYIILKWAQTI
                     NGFIAKENGDSKWISNEISRKLTHKWRTEEDAILVGAITAEIDNPKLTARNWTGKNPI
                     RVVLGNPKLHSEANLLNGESKTWIFNSFRTGMDMNLAWIKFGKSTMLKETLRYLFENN
                     VQSLIVEGGATTLKNFIDANLWDEARVFTSPAVFKSGIKAPELNRRGHVQAVLGDDIL
                     TITRNYNG"
     gene            8110..8964
                     /gene="prmC"
                     /locus_tag="VGA21_07870"
     CDS             8110..8964
                     /gene="prmC"
                     /locus_tag="VGA21_07870"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEX9650462.1"
                     /translation="MIAFMPSIKEIRNNIFDELSPKLGVEESTALIFILLEFVLKKEK
                     VFLLANPEVEIAPDELIFLENAVVRLINHEPIQYILEEAVFLGRRFYVNPSVLIPRPE
                     TEELVLLALKNCKGGERILDIGSGSGVIGISLALEIEASQVYCLDNDAASLQVAKRNS
                     MNHKVQIQLIHSDFLNEIPEINHFDIIVSNPPYIAESEKSLMQKNVLNYEPRKALFVP
                     DNNPLVFYSRIAALRKKLLSPKGKIFVEINEAFGLQIEEIFYEAGSLHVQIFKDINGK
                     DRLVYARF"
     gene            9047..10693
                     /locus_tag="VGA21_07875"
     CDS             9047..10693
                     /locus_tag="VGA21_07875"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015813892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEX9650463.1"
                     /translation="MKVSWKYISIIVFASYSAPGYSQTQPPVFTHQDSLRGSITPERA
                     WWDLTYYHLNISVNPGDSSITGFNTIQYRVVGSQGEMQIDLQPPLRINKVTQNGKILV
                     FRQDGNAWFVRLAESHEPESIKEVVIFYSGKPQIAARPPWDGGFTWSEDENGNSFIAT
                     SCQGDGASLWWPCKDHMYDEPDSMLISVTVPQDLMNVSNGRLRKIDTNRKQKTKTYHW
                     FVSSPINNYGVNVNIADYVHFSETYAGEKGDLDCDYYVLRNNLAKAREQFKDAARTLE
                     AFEYWFGPYPFYEDSYKLVEVPYLGMEHQSSVTYGNKYVNGYLGRDLSGTGWGLKFDF
                     IIIHETAHEWFANNITYKDIADMWIHESFAAYSESLYVQYHFGKDAGYEYIRGTRRNI
                     RNDKPIIGHYDVNNSGSGDMYSKGANMLHTLAQVINDDEKWRSILRGLNEEFYHRTVT
                     TNQIENFISEKSGRNLDAFFNQYLRTAMIPVFEFTQVDDNLLYRWTNVVDGFNIPLRI
                     FINGEESWLQPTNRWNSINGLEPDSQVLIDSNFYVATLNY"
     gene            complement(10690..13110)
                     /locus_tag="VGA21_07880"
     CDS             complement(10690..13110)
                     /locus_tag="VGA21_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009578693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX9650464.1"
                     /translation="MKSVILWMTLTMMVIPAYGQFSIAGKIIDAETEEPLVGANIVLR
                     ENSKGASSGSDGNFEIINLKQGKYLIIVSFIGYQDFEEEIELTRNINLNILLKPRALR
                     IEGFVVEATRVGENSATAYTTLDKEKLSKNNLGQDFPYLLDNTPGVVSFSDAGAGIGY
                     SGLRIRGSDPSRVNVTINGIPLNDSESQGVFWVDLPDFASSVQSVQIQRGVGTSTNGS
                     GAFGASINIETGLDSVEPLVQIDNSFGSFNTRKHTLVASTGLIKEKLNLNIRLSEIAS
                     DGYIERASSLLKSYFVSGTYSGIKNNIKLNIFSGKEVTYQAWDGVSDFNLQTDRTFNA
                     TGQIGPNSFYDNQVDDYNQNHYQLHYNYSAISNLALALSLHYTKGKGFFEQYRVDDDF
                     ADYGFNDIVIGTQVISSSDLIRRRWLDNDFYGTTFSAIYAPGSTFKVSFGGSANIYDG
                     DHFGEIIWAQFAGNSTNSDHYYDNTGRKTELNLYLKPEINISSQLFVFGDIQFRNVSY
                     EVNGIDNDQRILNTDKQFDFFNPKIGVSFRINSNTLAYASYGRANREPDRNDFIDSQD
                     GAGPKHETLNDFEAGFRYSASSLKSEINLYSMQYENQLVLTGELNDVGTPIRINVDQS
                     HRTGAELSFTWSPLDKLEFSGNAAYSVNKIKEFEETAPIFDPVFNYLRDSVITYADTD
                     IILSPSWVAFGQITCIPLSGLEFSFNGKYVAEQFLDNKSNNNRKLDAFLLNNLNMSFT
                     RNFSNSIFRKIRISLLLNNLFNVRYEPNGYSYFIFFDDGSSVFQDNYNFYYPQAGFNF
                     LGGVSLTF"
     regulatory      complement(13151..13245)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(13261..13806)
                     /locus_tag="VGA21_07885"
     CDS             complement(13261..13806)
                     /locus_tag="VGA21_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009580496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9650465.1"
                     /translation="MEKDHDFGTSKARRVVVIGPESTGKSTLAASLATHYETIWVPEY
                     AREFIDRLNREYVEKDLLDIAKGQIASEVNLESKAKRILICDTNLIVLKIWSEYKYGA
                     CNPEILKLINKRTYDLYLLTSVDVPWSDDPQREHPHLRDFFYTAYKNELIARNLPYVE
                     ISGEFYERKKRAIEAIDSLLL"
     gene            complement(13761..14387)
                     /gene="pnuC"
                     /locus_tag="VGA21_07890"
     CDS             complement(13761..14387)
                     /gene="pnuC"
                     /locus_tag="VGA21_07890"
                     /inference="COORDINATES: protein motif:HMM:NF016837.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HEX9650466.1"
                     /translation="MNEILKEIIDYATRIDLLEFGGLVFGLLAVWFLIKNNLWTWPSG
                     IIYVFISFVVFWKAKLYADFGLHVFYLVLNIYGWYYWVAGAKKTEESDKLPITQIKRS
                     WWLWLTIGSAISIVIMGGLLSQTDASVPYWDSTTTVLSVIGMWLTARKKLENWHFWLI
                     VNILSSGIYIYKEIYFYTALYLIYIGMAVAGYLSWRKIMISEHLKPGE"
     gene            complement(14456..15202)
                     /locus_tag="VGA21_07895"
     CDS             complement(14456..15202)
                     /locus_tag="VGA21_07895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650467.1"
                     /translation="MYNETYQNFLVGWDNIIVILSYIFLAGSILTSLYYFINVLRLSN
                     PKVKYDYINESEIKLFWRSMLLLTISGGLYLNTIFDETVRQEVIWFFVRLFVTASFTT
                     IFALIFNSLIKVYYPFQVQKKLNKLRHIPRINPNNGKEMTLLSEEVEDDYLDAKMIEE
                     EASHHVDYDVWLDESTGFTIIEKYDGASLAIECPSCGYRTLRVVNEEVIKSPTLETEG
                     ELLNYYQCSYCSHKERKTIITKKLAAKSTT"
     gene            complement(15420..16130)
                     /locus_tag="VGA21_07900"
     CDS             complement(15420..16130)
                     /locus_tag="VGA21_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009580491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HEX9650468.1"
                     /translation="MMKLRFSFLMIGLLITGIETKSQPSFVQEGIASFYADKFEGRET
                     ASGEKYRHNKLTAAHKTLPFGTLVKVTNLTNNKSVVVMVNDRGPFVEGRIIDLSRSAA
                     QDLDFVLYGLTKVKLEVIDEVAARESKYDPGKPVQVNPDVEEREFYEFSVSRFSPSGF
                     GVQIGSFQEMVNLVRLSDNLKKSYRKRVTVQVSVIKGVKIYKIIIGLEKARSKAEALR
                     KRLSKKYPDCFIVEFKNL"
     gene            16248..17720
                     /gene="guaB"
                     /locus_tag="VGA21_07905"
     CDS             16248..17720
                     /gene="guaB"
                     /locus_tag="VGA21_07905"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019990341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEX9650469.1"
                     /translation="MSTSNSKIFTEALTYDDVLLLPGYSEVLPRDADTRTQLTRNIHL
                     NIPIVSAAMDTVTEYDLAIAMALEGGIGFIHKNMSVAAQAEQVRKVKRSQSGMILDPI
                     TLGAEATVADANKIMREFKIGGIPIVDKENVLIGIITNRDLRFQNDNGVPITEVMTKE
                     KLITAPEGCNLSEAESILQEYKIEKLPIVNKLGKLTGLITYKDILKKKDRPNACKDDF
                     GRLRVGAAVGVTNDLLDRIAALIKAGVDVVSIDTAHGHSKGVIDAVKMVKQKYKDLDL
                     VAGNVATSAGAEDLVRAGADSIKVGVGPGSICTTRVIAGVGVPQLTAILEARKGIGKS
                     GIPIIGDGGVRFSGDMVKAIAGGANSVMIGSLLAGTEEAPGEVIIYEGRKFKSYRGMG
                     SVEAMEEGSKDRYFQDAEDDIKKLVPEGVVGRVPYKGMVTEVLYQLTGGLRAGMGYCG
                     ASTIEKLMDAKFTKITLAGVRESHPHDVQITRESPNYSSR"
     gene            17803..19851
                     /locus_tag="VGA21_07910"
     CDS             17803..19851
                     /locus_tag="VGA21_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009580480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing SpoIIE family protein
                     phosphatase"
                     /protein_id="HEX9650470.1"
                     /translation="MSSKNIIRLSTIIGIISWLVFVTSDLYILFGQINNLKIGIPAIT
                     PRFAFDLFILSMFFYYKHQAEKVETLNFIDLLWRVFATGLLTTVVSLAIKFLFFAFGN
                     TKLAEDVFLINLTYHINIGLIGAFLVSTLIVWEKLILYQKSKLLLKSWQVFQLTLLAS
                     LFINLFPFQSNSALSVISLTVLIVMGLVLSVNLKWVAYLNFKQKWKSILLILLVILYL
                     GFFLQNLFQYASGDNLSLNILENTFTIALFSFVFSYALFSLLVILFNLPTSSVFEQKL
                     EEVINFQKLSQSIQTEQSEDQVYDILLHSAINTVFADAGWIEIFEKGEGSPYHMLNIS
                     ENEVESIKKNIKNSKIKGILDTDTDKSLLTSKLSSVLKSTRFKSIVAYPVLVQGKQIG
                     TLALLKEVSEGFNREMTNIIRAFVNQAGISIENFRLLYEAIENERYKEELKIAKMVQK
                     GLLPKQLDMNSSFEIAAFSEAADEVGGDYYDAFTINKEKVALIIGDVSGKGTSAAFHM
                     SQMKGVFNSLAQLGLPPKDFLCNANTALSTSLDRSSFVTCSYFIVDIAARKLEFARAG
                     HCPTLYFDSQSEKSIFFEDKGLGLGILRNDEFGKFLEVRNMQFNSGDILVLYTDGITE
                     AKNMKKHEYGFERLGKLLQDNHSSSPTVIKDIIIKDLYNFCGQESLDDDYTMLIVKFK
                     "
     gene            19890..20234
                     /locus_tag="VGA21_07915"
     CDS             19890..20234
                     /locus_tag="VGA21_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017730073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX9650471.1"
                     /translation="MINLTHKIEGAIYIISVVGDVDASSSIQLDNALGQALEDNQTKI
                     LVDCSKLNYISSAGLGVFMSYIQDFENKNIKLVLCNLNEKVIGVFRILGLDQLLKIVD
                     TIEEGKKLIDEL"
     gene            20224..20646
                     /locus_tag="VGA21_07920"
     CDS             20224..20646
                     /locus_tag="VGA21_07920"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009580478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9650472.1"
                     /translation="MNYSYKVPCQKEKLKDIRSFVSEALRKHGVPEVQIATMVLAVDE
                     VCANIIIHTYKCDPDEYVEIIIKVDKKLNCVFEISDQGDKFDITQYQEPSMAEIIRNK
                     RKGGIGLILVRKIMDDIELVQKDGHHVCRLTKKLGKES"
     gene            20701..22635
                     /locus_tag="VGA21_07925"
     CDS             20701..22635
                     /locus_tag="VGA21_07925"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX9650473.1"
                     /translation="MGKIIFTAFFVALAFAAHSFQSGILFSIGENQTSKEEFAFFYKK
                     NSQSNPEAFARADIEDYLRLFIDFKLKVKQAEDLGYDTTANFTRELNIYLGQLTESFM
                     NSNTSEEKLYLEAYERMQFDIDASHILVRLSENPKPSDTLPAYQKARSIYDRAKSGEN
                     FGELAVQYSEEPGAAMSKGHLGYFTAFQMVYPFETAAYSIPVNGISTPVRSQFGYHII
                     KVNNRRPAMGTVTVAHIMLRFNRDMQSPDSLEVRQKIDMVYDDLTKGASWDSVCVKYS
                     EDINSKVNGGRLRPFGIRGLNVPEFELAAHETPVAEISDPFKTPFGWHVLKVIGKNTI
                     GTFEEERAGIVRKVQNDARSRLPKEEWLSHLKERYKFEEKNDLSEKVTFLYDSTQSPV
                     GSVILFTLEDQSYSQLDLEAYAKSRNILDGAGALLWYNKFIEDELRNREEKFLLQNNL
                     EYRMLRNEYYQGILLFSIMEDKVWKKAERDSVGLRNYYKNNSDRFLVAGKKGIVISVE
                     PDSLTDQVFNSLLLNFKDLHANYDISTVKNWTDAVLSSQSRLTLRFAEKVFQEGNSAL
                     ENIDSGINLITNDGTQAILVLADETGNWVGQFEDVKGLAISFYQDILEENWINELHNN
                     YKVKINKKVLKQVINELESR"
     gene            22691..23509
                     /locus_tag="VGA21_07930"
     CDS             22691..23509
                     /locus_tag="VGA21_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009580476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEX9650474.1"
                     /translation="MKNSDVVEESTQSEPIARVLDHYLYPEDLAGIIGPGVSKEDSAN
                     LVLRYIKSWARKQLLINQAAANIDFNEAELERKILDYRYALMVYEFEKQYVNQEINLE
                     LSPQEIRDYYEKNKDKFELKQNIIRGVYVKIPNQAPRLVQVKNWVLSNKQKDLEELRS
                     YCLRFATNYSLEDSVWLNFDEVIRGTPLTAITNKVEFLRKNRFVESTDDEFNYLIRID
                     EYRITDQISPLTFVEDQIRSIIINKRKVELTRNLEDKVFNEAVTKKDFEVYVKE"
     gene            23496..24827
                     /locus_tag="VGA21_07935"
     CDS             23496..24827
                     /locus_tag="VGA21_07935"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017730069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX9650475.1"
                     /translation="MLKNKLFLLVGLFATHSLSFAQTNGLVIDKIIAKVDNHIILKSE
                     LELGYLDMITRGISVDDNSKCGVLEGLVLNKLLLAKSEIDSISVLDFEVERSLDNKLR
                     YFISQIGSEEKLEEYYGKSMEQFKDELRDQEKEQLLIQRMSESITEDVTITPGEVRRF
                     FNRIPIDSLPFFSKEVVVGEIVMYPKAGQERKDQAKELLRALKERILSGESFNQLASQ
                     YSQEPAASRSGGELGFFRRGELAHEYEAAALYLQPGEISIPVETEFGIHLIQLLERRG
                     NEYNSRHILRIPAPSQEDIQRTVNKMDSLWKMITLDSIKFEKAARDFSENKLTNINGG
                     FMQDQTGSDRISVEQLDPNTFFTLDTMHVGSISPPIVYKDEAGKDAVRLVFFKNSIRP
                     HRANLNDDWSKIRIAALNEKKQIVLDEWFEKSRFEVFIRIDAEYDNCNIMN"
     gene            24860..>25761
                     /locus_tag="VGA21_07940"
     CDS             24860..>25761
                     /locus_tag="VGA21_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009580474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX9650476.1"
                     /translation="MKEITNEVGAADKLHEAYQSLKKEIGKVIIGQDEVVRLVLTSIF
                     CQGHCLLVGVPGLAKTLLIQTISSALDLKFSRIQFTPDLMPSDILGAETLDKDRNFQF
                     MKGPVFANIILADEINRTPPKTQAALLEAMQEYSVTIAGKTFHLERPFFVLATQNPIE
                     QEGTYPLPEAQLDRFMFNILLDYPSYEQEVEIVKGTTTDSGISVGKILTHEEILGYQK
                     LVRMVPVPDNVIQYAVDLVHKTRPFTSRGEKLSDDFLEWGAGPRASQYLVLGAKANAL
                     LNGKYSPDIEDIQAVAKPTLRHRIV"
BASE COUNT         7727 a         5046 c         5303 g         7685 t
ORIGIN      
        1 ctcactaccg aggagcggta gtaatccagc acctggatgg tgaaccggta gaagccttcg
       61 ggcagcatgc ccagtcgctg gtaatcccgc ttgctgatgc ccagtaagtc caggttttcc
      121 aaactgaaat agggttgcag atcttctgcg gtcaatatat aaggtgtgcc gccactgagt
      181 ggtatgggct ggggcaaaaa ggtggctttg gtttttatgg tgatgccaac gccctccact
      241 atcagcctga gcttcaccgg gtactgattc ttggttaaat cagtcagcag tagttgtacc
      301 tgcagcagag tactgcccgg cattccatac tcatagagag ccagggaata gggcggattc
      361 aggaaggccg atacctgaac gggggtttgt tgtgcaaatg cccggattcc aaaaaacaga
      421 attgcgcata aaaaagccct gtatttcaaa tcgtttagat tttttgaatt gacagggttt
      481 agctaaatct attacttgct tagattttag tgttttgaag gtagggagct tattcttaaa
      541 atgcaaataa ttattttata ttttatatat accgatctgc tacttctttt attttctttc
      601 catcttcaaa atgttcctca aaatgctccc aactcagtct tgagttacta cgaaccggga
      661 caggatttat cattattttt ttgcgttggc tataaaaacc ccggctaaaa caattatcat
      721 ccccagaaag tgtccgatga gtaatacctc cccgtcccat actccccaac ccactgcaac
      781 cagggggatc aggtaggtta cggaggaagt aaaaatgggg tttgtcattc gaaccaaacc
      841 attgaaaata accagggcaa tcgaagtgct cataataccc aaaagtgtta tcgcagcgaa
      901 agcgaggctt gcatcaggga ttgtggtcat ctttgaaatg aagtctgttc cgaacagcag
      961 aaacaaggtt gcgggtatca gaaccagtga aatgctgatt gaggtaatgc ttattgcttt
     1021 aagactttca attttatact tgatgatatt caaattgaat ccatagcaaa tggttgctgc
     1081 caccacgaac agaccgtaga agtttaattg gtcccacgac cctccggaac ctgccatcac
     1141 cagtaaggct gtgccacaaa atcctattat cagtcctatg tactgatttc gtctgatctt
     1201 ccgggcaaaa aatattgcac cgataaaaag aacgaataat ggggttaatg catttattac
     1261 acctgccagt gagctgtcaa tcctggtttg cgctttcgcg aaaaggaaag caggtatcaa
     1321 acttcccatt aaaccgatgt acaggaggta tttccactcc cgttgattca ctttgcgtat
     1381 gtctttaaat gcccagggaa gtaaaaatac tcccgctgcg gctatcctca atgagccgac
     1441 ttccagtgga tcaaaaaccc gtaagcctgc ttttattaaa atgaatgatg aaccccacat
     1501 taacgacaaa agaatcagaa gcaaccaggc tttcaacccc ggtttttctg atggggcgtt
     1561 gttaatcata ctgcatagga ttcctgatag agtccttcga tttcttccag aatgctgtta
     1621 atctcataaa atgactcaga ctgaacaatc ctgattctta aaggtttaaa attatctacg
     1681 ccacgaaaat aactagtata atgtcttctc atttcatgga tgcctttctt ttctcccttc
     1741 cactccatcg agagttctaa atgttttctt gccactttca ctctttcctt tatcccgaca
     1801 tccggcagtt tttctccggt ttttatgtaa tgcttgattt gattaaaaat ccagggatta
     1861 ccgatgcttg ctcttcccag cattattcca tctactccat attgcgattt ataatccaat
     1921 gctttttgcg gtgaatctat gtcaccgttt ccaaaaattg gaatgattat gtcaggatgg
     1981 gattttacat tggctatata actccagtcg gctgcacctt tatacatttg ctgccttgtt
     2041 cttccatgga tagttaatgc tttgatacct gcatcctgaa gtctcaaggc tacctcaact
     2101 attttgatag aagtatcgtc ccagcctaac cgtgttttta cggtaaccgg aagagaaacc
     2161 tgcttaacta tttcgctggt catcgcctcc atctttggca aatcctgtaa tattccggca
     2221 ccggcgccct tgcaagcgac ctttttgacg ggacatccgt aattgatatc aattaattca
     2281 gggcgcgcat tttcagcgat ctcagccgcc tgcctcatcg attcaatctt atccccaaaa
     2341 atctggatac caatgggacg ttcatagtca taaatatcaa gtttctgcat gcttttgtcg
     2401 gcttccctga tcaggccttc agcagaaatg aattcagtat acatcaaatc tgctccattt
     2461 tccttacata cagctctgaa aggaggatca cttacgtcct ccattggagc caaaagcaaa
     2521 gggaaatctc cgaggttgat gttaccaagc gttaccacaa gttttgaatt ttcggttaaa
     2581 tttactgcaa ttatggaaca taacggcaat gaactcgcct tgcaaaattc ccggaagccc
     2641 gcactctcgt tcatgctggt tctgtttttt tcgtttgttg gctttacaac tctgggccca
     2701 ttccttggaa ttctctttgc cttaccgttc cccggatttg atttgaataa ccttttgagc
     2761 attcttgaaa atccggtgaa gtttccggaa tctaaaactc catttctaat tatccagggg
     2821 ataagttccg tggtaggatt tattgtttta ccctctatat ttctggtggg aatacaaaag
     2881 atgggtttca ggatcttttt tcaagccaaa accgtaactc cggttgtcat ctttgtaacc
     2941 attttgttca ttatcctttt catggttgtg aattcggtgt ttgtagagtg gaatgcaaac
     3001 ctcaaattgc cggagttcat gagcggattt gaaaactggg ctcgtggcct tgaagataat
     3061 gccgcggtat tgacccaatt tttgacagac tttgataata taggccaatt cttgattgct
     3121 tttgtcgtga ttgccattat tccggccttt ggcgaggaat ttgttttcag aggattgctg
     3181 caaaattatg ccataggatt tttacgaaat cctcatgtag ccatatgggt aaccgccatt
     3241 ctgttcagtg gatttcacct tcagtttttt ggctttgtac cccgagcttt cttaggagtc
     3301 gcgtttgggt atctatattt ttggtctggt aacttaacaa ttccgataat agcgcacttt
     3361 attaataacg gtttcacttt attgatgata tacatgtaca gaacagatgc aattgggttt
     3421 gatattgaga ctactgaatc accaggtgtg ttaaccgtaa ttcctatggc ttttataaca
     3481 gccaccttgc tctatttctt tcacaaacac actttccaaa taagaaagca acatggcgac
     3541 atggcaaacc gtgtttaaat ctgaaaattc tgtgagagca gaaattgtta aagatgttct
     3601 gctgggaaga gacattaccg ctttaattgt aaataagaga gactcttcgt ataatacttt
     3661 cggatatttt gaagtggttg ttcataatga caacgtgatt gaagcattaa ggataattga
     3721 agatgagatc gtttttgagt aagtatagta attttaccca aagagttgtc acggggttga
     3781 ccggtgcggt tattcttatg acagcccttt cctggagtga atggggatat tttatctgct
     3841 tccttctggt tgtggtgatt tcacaacttg aattttataa gttagtcgga ttagatggaa
     3901 tgtctccact taaattctgg ggaacgttca ccgggctttc cgtatttgtg ctatcatttt
     3961 tgatcgaaag aggttctgtc aacaccaatt tttattacct tatttttccg attgcatgcg
     4021 gcactttctt tattaaactt tataagaaat cagataagaa gcctttcact aatattgcgt
     4081 tcaccttaat cggagttatt tacattgccc taccaatagc tttgttaaat atttccactt
     4141 ttgtcatggg tcagtatagc catgaaatca ttattggttt gattctaata ttttgggcca
     4201 gcgatattgg cgcttatttc gcgggaatta aattcggtaa acgaaaactt tttgaaagaa
     4261 tctctccgaa gaaaagttgg gaaggcgcgc ttggaggtgc ggcatttgct ttactggttg
     4321 cctacctgat aagcttatat tttaccgacc tgacatttat caactggatg attttaggtg
     4381 ccatcattat tgtgggtgga acttatggtg atttaattga atcccttttc aaaaggagta
     4441 tagaaattaa agattcagga acaaaattac ctggccacgg aggtttttta gaccggtttg
     4501 acagtttatt aatcgcttca ccattcttag tcgcttacct taagctgttt ctctgaatta
     4561 attatgcaag gcaccaacgc tttgcctgat atttgctcta actttgcaac cgatttgata
     4621 aaatcttttt taataagaat atgacttaca ttgaaccggc tccgataaag gacgccgaaa
     4681 acccatttga gtcaatgatg tcaaggtttg aaattgcttc cagaatactg ggccttgacg
     4741 atgaagtgta taacgtttta aagactcccg caaagcaagt tgttgtttca ttacccatca
     4801 cgatggatga tggttctatc aaagtttttg aaggataccg ggtaattcac tccaaaattt
     4861 tgggcccttc caaaggtgga gtgcgctatg atatggcagt aaacctggat gaagttaaag
     4921 ctcttgccgc gtggatgacc tggaaatgcg ctgtagtgga tattccctat ggaggcgcca
     4981 agggtggcat taaatgcgat ccgcgatcaa tgtcggcagg agaaattgaa cgattgaccc
     5041 gggcatacac gggagcattg attgaaattt ttggaccgga cagagatatt ccggcaccgg
     5101 acatgggaac cggtccaagg gaaatggcat ggctaatgga cgaattctca agagcccagg
     5161 gcatgacagt taacgccgta gtaacaggaa aaccgctggt attgggagga tctttaggaa
     5221 gagtagaagc cacaggacgc ggagtaatgg tatcagcact agctgcaatg gaaaagctta
     5281 aaattaatcc atatcaggca acctgtgcag tccagggatt tggaaatgtt ggaagctacg
     5341 cgtcactatt attagaagaa cggggtgcca gagtagttgc gattagtgat atcagcggtg
     5401 gttattataa tgcaaatgga attgctatcc gggatgccgt tgattataga aaccaaaata
     5461 acaattctct tgatggattt aaaggcggcg attttatttc caatatggat atccttttgc
     5521 ttgatgttga tgttattgtg ccagctgcca aggaagatgt tatcactatc aaaaatgcgg
     5581 aacaaataaa agccaaactg atagttgaag gggcgaacgg tccaacgtcc gctaaagcgg
     5641 atgcaattat taatgaaaaa ggaattatgg ttgctcccga tatcctggca aacgccggag
     5701 gtgtcactgt ttcttatttc gaatgggttc aaaacaggct tggattcaaa tggaccagcg
     5761 aacgggttaa caggcgttca gaccgaatca tgaaagatgc ttttgatcgg gtgtataaaa
     5821 catcaattga ctatagtgtg tctatgagaa ttgcagccta cattgtggct atagataaag
     5881 tggccaagac ctacaaatgg cgcggcgggt tttagtatca tagcaatgaa aattcataaa
     5941 gaaggtaaaa acttactttt tgttttacta ttacttctaa ttgcaataga cgcggtcgcc
     6001 tacaatttca tcgtgtcaca agcagccctt tggattgtaa ttggctttag tattatcatt
     6061 tttcttcttg tgttacaatt tttcagaaat ccaaccgtgg tagttcccat tgatgagaag
     6121 catatttatg cccccgcgga cggcaaagtg gtagcaattg aaagagttga agcaaatgaa
     6181 tttatttcaa tcccttccca gcaaataagt atttttatgt ctccggtaaa tgttcatgtg
     6241 aaccgggccc cggtttcagg gaaagtgaaa ttgtttcgct atcataaagg aaggtatctg
     6301 gttgcctggc accctaaatc cagttctgag aatgagcaaa caacgatgat tattcaaatg
     6361 agcaatggag tggaaatact agtaagacaa atcgcgggtg cactcgccag aagaatcaaa
     6421 tggtatgttg aagaaggaga tgatgtaatc cagggaaatg aattcgggtt cataaaattt
     6481 ggaagccggg tagatatatt aatcccggag gattttacta ttacagctca gataggagaa
     6541 aagaccaaag ccggcaaaac catcattgcc accgtaccta attaggattt tgcgataatt
     6601 tgctctacaa tatcagtgag atatttttga tctactaaat caaaatcact acgcttatcg
     6661 ctgtccacat caagaagcag aatcatttca ccatctttaa ttccagggat gacgatttcc
     6721 gactttgata agctgctaca acttatatga cctggaaact ggtctacgtc aggaacgata
     6781 attggcgctt tgtttttcca actggttcca cacactccca tacccttctg aatccttgtg
     6841 caggcaacag gtccctgaaa tggtcccaat attaattcat caccctccac aaggtagaat
     6901 ccaacccaga aaaagttcat agcttccttc aatgcggctg aagtattcgc cagatttgca
     6961 atcaaatttt tttcattatc aattaatgct ttgatctgcg gtattaaact ttcataccgt
     7021 ttcttcttgt ctctcgattt acttataatt aattcctcag ccattgtaat ttcgtgttat
     7081 cgttaatata tcatcaccca gtacagcttg tacatggccc ctccgattca attccggagc
     7141 tttaattcca ctcttaaata cagcgggcga agtaaacacc cttgcttcat cccataaatt
     7201 agcatcaata aagtttttta aggtggttgc tccgccttct actattagtg attgtacatt
     7261 attttcaaag agatatcgca atgtttcctt cagcatcgtt gatttaccga attttatcca
     7321 cgcaaggttc atatccattc cggtgcggaa agaattaaag atccatgttt tggattctcc
     7381 gtttaacaag tttgcttctg aatgaagttt agggtttccc agcactacac ggatagggtt
     7441 tttacccgtc caatttcttg cagttagttt cggattgtct atctctgcgg ttatagctcc
     7501 aactaaaatg gcatcttctt cagtgcgcca tttgtgtgtt aatttcctag agatctcatt
     7561 gcttatccat ttcgaatcac cgttctcctt tgctataaat ccgttaattg tttgagccca
     7621 tttcagaatt atataaggtc tcttctctaa atgaaaagtg acaaacctgc ggttcatttc
     7681 ctcagcctgt tcttccatgt accccaactt tactttgatt ccgctacttt caagttgacg
     7741 gattcccgta ccattcacca gcacgttcgg atctttcaat ccaataatta cctctttcac
     7801 tcctgactct attaatacat tagtacaggg tggagttttc ccgatatgag aacagggctc
     7861 aagggttaca tagactttgc ttcgattcaa tatctttttg tctttcatgc tttcaattgc
     7921 attaacttca gcatgaggtc cgccaaagta ttcatgccaa ccctctccaa ctacttcacc
     7981 cttatgggct ataacgcacc caaccatagg attaggactc actttaccga gtccccgtaa
     8041 ggctagatcc atagcccgtt ccataaattt tctttccgct tccaatgctt caaaattacg
     8101 ttaattttga tgatagcatt tatgccatcc atcaaagaaa tcaggaataa tatttttgac
     8161 gagcttagcc ctaaacttgg ggttgaggaa agtacagctt tgatatttat tctccttgaa
     8221 tttgtcttga aaaaagaaaa agtttttctc ctcgccaatc cggaggttga aatcgcacca
     8281 gatgaattga tatttttaga aaatgcggtt gtgagattga ttaatcatga acccattcaa
     8341 tatatccttg aagaagctgt ttttttgggg aggcggtttt atgtgaatcc ttctgtgttg
     8401 atccccagac ctgaaacaga agaattggtt ctactggcct taaaaaattg caagggtgga
     8461 gaaagaattt tggatattgg atcagggagt ggggtaatag gaatctcatt agccctggaa
     8521 attgaagcca gccaggttta ttgcctggac aatgatgcag catcattaca ggtagcgaaa
     8581 aggaattcta tgaatcataa ggtgcagatt cagcttatac actcggattt tttaaatgaa
     8641 attccggaaa taaaccattt tgatattatt gtaagtaatc cgccttatat cgccgaatca
     8701 gaaaaatcat tgatgcagaa aaatgttctc aattatgaac cccggaaagc attgtttgtg
     8761 ccggataata atccgttggt tttctattcc aggatagcag ctttaagaaa gaagttatta
     8821 agtccgaaag gcaagatttt tgttgagata aatgaagcct tcgggctaca aattgaggaa
     8881 atattttacg aagctggttc ccttcacgtg caaatcttca aggatataaa tggtaaagac
     8941 agacttgtct atgcccggtt ttgaataatt cttatgactc aagcaaaaaa accggttgaa
     9001 ttatcggaaa ccttacttac tttatatcga aatttaattt tataggatga aagtctcctg
     9061 gaagtatatc tcaattatag ttttcgcttc ttattctgca ccgggctatt cacaaactca
     9121 accacccgtg ttcactcatc aggacagcct gagaggctcc ataacccctg agcgtgcctg
     9181 gtgggacctc acttattatc acctaaacat tagtgttaac ccaggtgata gttcaattac
     9241 cggtttcaac actatccagt acagggtggt ggggagtcaa ggtgaaatgc aaattgactt
     9301 gcagccaccc ctccggataa ataaagtcac tcaaaacgga aagatcctgg tctttcgtca
     9361 ggatggaaac gcatggtttg tcaggcttgc cgagtcccat gagccagaaa gcattaaaga
     9421 ggtagttatt ttttatagcg gcaagccaca gatagcagcc aggccgccat gggatggcgg
     9481 atttacatgg tctgaagatg aaaatggaaa ctcttttatc gctacgtcct gccagggtga
     9541 tggagccagt ttgtggtggc cctgcaaaga tcatatgtat gatgaaccag acagtatgct
     9601 cataagtgtt acggttccgc aagatttgat gaatgtatcc aatgggagat tgaggaaaat
     9661 tgataccaac cgaaaacaaa aaacaaaaac ctatcattgg tttgtttcaa gtccaataaa
     9721 taattatgga gtaaatgtaa atattgccga ctatgttcac ttttcagaaa cgtacgccgg
     9781 agagaagggc gaccttgatt gtgactatta tgtgttgcga aacaatcttg ctaaagcaag
     9841 agagcagttc aaggatgctg caagaaccct ggaagctttt gagtattggt ttggtcctta
     9901 tccattttat gaggattcat ataagttagt agaagtacct tatctgggaa tggagcatca
     9961 aagttccgtt acgtatggca ataagtatgt caatggctac ctgggcagag atctctccgg
    10021 aacaggctgg ggattaaaat ttgatttcat tatcatccat gaaactgccc atgaatggtt
    10081 cgctaacaat attacgtata aggatattgc agacatgtgg attcatgaat cttttgcagc
    10141 ttactcagaa agtttgtatg tacagtatca ttttggtaag gacgccggat atgaatatat
    10201 acgaggaaca agaagaaata ttcgcaatga caaaccaatt atcgggcatt acgatgtaaa
    10261 taatagcggt tccggggata tgtacagcaa gggagctaat atgctgcata ctttggccca
    10321 ggttattaat gatgatgaaa aatggcgtag tattcttcgg ggcttgaatg aagagttcta
    10381 tcatcgaaca gtaactacaa atcagataga aaactttata agtgaaaaat caggccgtaa
    10441 cctggatgcg tttttcaatc aatatctccg caccgcaatg attcctgtat ttgaattcac
    10501 ccaggttgat gataatctgt tataccgatg gaccaatgtg gtggacggat tcaatattcc
    10561 actaagaatt tttattaatg gtgaagagag ctggttgcag cccactaacc gatggaattc
    10621 cattaacggg ctagagcctg attcccaggt tctgattgat tcaaattttt atgtggccac
    10681 tttaaattat taaaaagtca gggaaactcc cccaaggaaa ttgaaccctg cctggggata
    10741 gtaaaagtta tagttatcct gaaaaaccga gcttccatca tcaaaaaata taaagtaaga
    10801 gtacccgtta ggttcatacc tcacattaaa caaattattt aacagtaaac tgattcttat
    10861 ttttctgaaa atcgaatttg aaaaattgcg ggtaaacgac atgttaagat tattgagaag
    10921 aaaagcatcc aacttcctgt tgttgttaga tttattatcc aaaaattgct cagcaacata
    10981 ctttccatta aaggaaaatt ccagtccgga aagaggaatg caggtaattt ggccaaaggc
    11041 cacccaggat ggtgacagaa tgatgtcggt atcagcataa gtaataactg aatctctcag
    11101 ataattgaat acgggatcaa aaataggagc ggtttcttca aattctttaa tcttattaac
    11161 actgtacgcc gcgtttccgc taaactctaa tttgtccagc ggactccagg tgaatgataa
    11221 ctccgcgcct gttctgtggc tttgatccac atttattcga atgggtgtgc caacatcatt
    11281 caattctccc gttaaaacca attgattttc atattgcata ctatataagt tgatttcaga
    11341 tttcaaggag gaagccgagt agcggaaccc cgcttcaaaa tcatttagag tttcgtgttt
    11401 tggtcctgct ccatcctgtg aatcgataaa atcatttcgg tccggctccc ggtttgcccg
    11461 gccataactg gcataggcca gcgtgtttga attgattcta aaagaaactc caatcttggg
    11521 gttgaaaaaa tcaaactgct tatctgtatt caaaatcctt tggtcattat ctattccgtt
    11581 tacttcataa gagacattcc tgaattgaat atcaccaaaa acaaacaact gtgatgagat
    11641 attgatctcc ggcttcagat acaaatttag ttctgtcttc ctgccggtgt tatcgtaata
    11701 atgatctgaa tttgttgagt tccccgcaaa ttgagcccag ataatctccc caaagtgatc
    11761 accatcgtag atatttgccg acccgccaaa tgataccttg aaagttgatc ccggagcata
    11821 tatggcactg aatgttgtac cgtaaaaatc attatccagc caccttcgtc gaatcaaatc
    11881 agacgatgaa attacttgtg taccaatcac aatgtcatta aatccatagt cggcaaagtc
    11941 atcatccacc ctgtattgtt caaagaatcc ttttcctttt gtatagtgca aagaaagagc
    12001 cagagcaaga tttgaaattg ctgagtaatt gtaatgaagc tgatagtgat tctggttata
    12061 atcatcaacc tgattatcat aaaaagaatt tggtccgatc tgtcctgttg cattaaaagt
    12121 tctgtctgtt tgcaaattaa aatcgctcac cccatcccat gcctgatagg taacttcttt
    12181 tcctgaaaaa atattcagtt taatgttgtt tttaatgcct gaatatgttc cggaaacaaa
    12241 ataggatttg agtagtgatg aagctctttc gatgtaacca tcacttgcta tctcacttaa
    12301 cctgatattc aaattgagtt tttctttaat caaaccagta ctcgcaacca gggtatgttt
    12361 tcttgtattg aaagaaccaa atgaattatc aatttgaaca agcggttcca cggagtctaa
    12421 tccagtttct atgttaatgg atgctccaaa agcacctgaa ccattagtag aagtacccac
    12481 cccccgctga atctgcacgg attgcaccga cgaagcaaaa tcaggtaagt ctacccagaa
    12541 taccccctgt gattcactgt cattcaatgg aattccgttg attgtgacat ttacgcggga
    12601 gggatcactt ccccggatgc gcaacccgga ataaccaatt ccggctccgg catctgaaaa
    12661 tgaaacaaca ccaggagtat tatcaagcaa gtaaggaaag tcttgcccaa gattattttt
    12721 tgataacttt tccttatcca gagtggtgta ggcggtggca gaattttctc caacccgggt
    12781 tgcctcaaca acaaagcctt caattcgaag agctctgggt ttaagcaata tattcaagtt
    12841 tatattccgg gttaattcaa tttcctcttc aaaatcctgg taacctataa acgaaacgat
    12901 tattagatat ttcccttgtt taagattgat tatttcaaag tttccatcac tacctgatga
    12961 agcacctttt gaattttcgc ggaggacaat gtttgcccca accaggggtt cttcagtttc
    13021 cgcgtcaatg atttttccgg caattgaaaa ttgcccatag gctggtatga ccatcatcgt
    13081 gagggtcatc caaaggataa cagatttcat aatatttcat ttttggttaa cacccgaggt
    13141 tggggaatcc tcatttgttt accatactcc cttcgcctgc attaccagga tcaggtattt
    13201 tgggtatgat ctcagcccgg ttttagggca cccccgcatc acaaaggtaa aaaggaagag
    13261 tcaaagcaac aaagaatcga tggcttctat tgcgcgtttc ttccgttcat aaaactctcc
    13321 actgatttcc acgtatggga gatttcgtgc gattaattcg tttttatagg cggtataaaa
    13381 aaaatcacgt aagtgaggat gttcacgctg gggatcgtca gaccacggaa catcgacaga
    13441 ggtaagcaaa tacaaatcgt aggtacgctt gttgattagc ttcaggattt ccggattaca
    13501 tgcaccgtat ttatactcgc tccagatttt caacacaatt aaattagtgt cacaaatcag
    13561 aatccttttt gcttttgatt caaggtttac ttcggaagca atttgacctt tcgctatatc
    13621 aagaagatcc ttctccacat attctctgtt cagacgatct atgaattccc gggcatattc
    13681 gggtacccag atagtttcat agtgtgttgc cagggatgcc gccagtgtac ttttcccggt
    13741 tgattcagga ccaataacaa ctactcgcct ggctttagat gttccgaaat catgatcttt
    13801 ctccatgata aataacctgc tactgccatt ccaatataaa ttaaatagag ggcggtatag
    13861 aaatagattt ccttataaat ataaatcccc gatgaaagta tgttgacaat aagccaaaaa
    13921 tgccaatttt caagcttttt gcgggcagtc agccacatgc caatcacgct cagtactgtt
    13981 gtggtgctgt cccagtaggg taccgatgca tcagtttgtg agaggagacc tcccataatt
    14041 actatggaaa tagctgaccc aattgtcagc caaagccacc aacttctttt gatttgagtt
    14101 attggcaatt tatcagactc ttccgttttt ttggcacctg caacccaata ataccagcca
    14161 tatatgttca acaccaggta gaatacatga agaccgaagt ccgcatatag tttagctttc
    14221 caaaacacca caaaggaaat aaatacatag atgattcctg aaggccaggt ccaaagatta
    14281 ttcttaatga gaaaccatac tgccaataga ccgaagacca agccaccaaa ctccagcagg
    14341 tcaattctgg tagcgtaatc aatgatttcc tttagaattt cattcatgaa agagtattaa
    14401 ggagcaaaaa aaaagccgcg cttctgcgcg gctcatcaaa atcttaattc tttacctatg
    14461 ttgtactctt agcagcaagt ttcttagtga taatagtctt gcgttccttg tggctgcaat
    14521 aagaacactg atagtaattc agcagttcac cttcggtttc cagggttgga gattttataa
    14581 cctcctcatt cacaacacgc agggtacgat atccacatga aggacactca atggccaggg
    14641 aagcaccatc atacttttca attattgtaa acccggtaga ttcatcaagc cagacatcgt
    14701 aatcgacgtg gtgagatgct tcctcttcta tcatcttggc gtcaagatag tcatcttcaa
    14761 cctcctcact cagcaatgtc atctctttac cattgtttgg attaattctt gggatgtgac
    14821 ggagtttgtt gagtttcttt tgtacctgaa aaggataata aacctttata agactattaa
    14881 atatcaaagc aaaaatggtt gtgaaactgg cagtaacaaa caatctgaca aaaaaccata
    14941 taacttcctg ccgaacagtc tcatcaaaaa ttgtgtttag ataaagcccg cctgaaatgg
    15001 tgaggagcag catagacctc caaaaaagtt ttatttcact ttcgttgatg taatcatact
    15061 taaccttagg gttgctgagc ctcaatacat taatgaaata gtatagcgaa gtcaatattg
    15121 atcccgctag aaaaatgtaa gataagatta caatgatgtt atcccatcca actaagaaat
    15181 tctgataagt ttcgttatac attccgtttt gtggttgatg ttgaaataat gaagagattt
    15241 tcggtactta aaataaatca aattagcaaa gaccgccaaa gaaaaaagca atttttgcat
    15301 tcttacaata ataaaaaaat ttgatcgaaa aattttatgt ctgggttgta atttactcgt
    15361 aatcgctcct atgaatcttt caaaattatt actttttatt ctaattatta tatcgcgctt
    15421 tataaatttt tgaattccac gataaaacaa tcaggatatt tctttgataa ccgctttctt
    15481 aatgcctctg ccttgcttcg agccttttcc agtcctatga ttatcttata aattttaaca
    15541 ccttttataa cacttacctg cacggttacc ctttttcggt aggatttttt caagttgtcc
    15601 gagagtcgaa ccaggtttac catttcctga aaacttccaa tctgaactcc aaaaccgctg
    15661 ggtgaaaaac gacttaccga gaattcatag aactcccgct cctctacatc cgggttcacc
    15721 tgaacaggct tcccgggatc atatttggac tccctggctg ccacctcatc aattacctcc
    15781 aatttcactt ttgtcagccc atataacaca aaatccaggt cctgagcagc cgatctggag
    15841 agatcgatga ttctgccttc gacaaaaggg ccacggtcat ttaccataac tacaactgat
    15901 ttattattag ttaggtttgt tactttaacc aacgtcccaa atggcagggt tttatgagct
    15961 gcagttagtt tattgtgtcg atatttttca ccactcgccg tttcccttcc ttcaaactta
    16021 tccgcataaa acgatgcaat tccttcctga acaaacgagg gctggctttt agtctctatg
    16081 ccagttatta gcaaaccaat cataaggaag gagaatctca atttcatcat aacaaatttt
    16141 tgagaaccaa attatagcgg atattaaaat ttttcaaaaa cttactcata aatgaccagt
    16201 ttgtagctaa ctttgaccgg caaataaggt atcataatta tttgctcatg tctacaagca
    16261 attccaagat ttttaccgaa gcattgacct atgatgatgt acttctgctt ccgggttatt
    16321 ctgaggtcct tcccagggat gcggatacac gaactcaact gaccagaaat atacatttga
    16381 acattcctat cgtttcggca gctatggata cggttactga atatgacctg gccattgcca
    16441 tggctctcga agggggaatc ggttttatcc acaaaaatat gtcagttgcg gctcaggcgg
    16501 agcaagtcag gaaagtaaag cggtctcaaa gcggaatgat tctcgacccg attacccttg
    16561 gagcagaagc aactgttgca gatgcaaaca agataatgcg tgagtttaag attggaggta
    16621 ttccaattgt tgataaagaa aatgttctca taggaataat cactaaccgg gatctgcgat
    16681 tccagaatga taatggagtt cctattactg aggttatgac caaagagaaa cttattacag
    16741 ccccggaagg gtgtaatttg agtgaggcgg agtctatttt gcaagaatat aaaatcgaaa
    16801 aacttcccat tgtaaataaa ctgggcaagc taacgggttt aatcacgtat aaagatatcc
    16861 ttaagaagaa agacagacca aacgcctgca aagatgattt tggcagactc cgggtgggag
    16921 cggcggttgg agtaactaat gacttattgg atcggattgc cgctttaata aaagccgggg
    16981 ttgacgttgt tagtatcgac accgcgcatg gtcactcaaa aggggttatt gatgctgtta
    17041 aaatggttaa acaaaagtat aaggatttag atcttgttgc aggaaatgtg gcaaccagcg
    17101 ccggagcgga agaccttgta agagcaggtg cggattctat caaggtgggg gttggtccgg
    17161 gaagtatttg tactaccagg gtaattgccg gtgtcggtgt tccgcagcta acagcaattt
    17221 tggaagctcg aaagggaatc ggtaaatccg gaattcccat tattggtgat gggggagtca
    17281 ggttttccgg tgatatggta aaggccattg cggggggtgc caacagcgta atgattggat
    17341 ctttgcttgc cggaactgaa gaagcaccgg gtgaagttat tatttacgaa ggaagaaagt
    17401 ttaagagcta ccgcggaatg ggttccgtgg aagcaatgga agagggttcg aaagatcgtt
    17461 atttccagga tgcagaagat gacattaaaa agcttgtccc ggaaggagtt gtgggtcgag
    17521 ttccttataa aggcatggtc accgaagttc tctatcaact cacaggcgga ttgcgagccg
    17581 gcatgggtta ttgcggggcc tccaccattg agaaactgat ggatgcaaaa ttcaccaaaa
    17641 tcaccctggc cggtgtcaga gaaagccatc ctcatgatgt tcagattacc cgtgagtctc
    17701 ccaattacag ctcaaggtaa gaccgtttac cacctcattt agttgtaatt agtggaatta
    17761 gcagaatatt tgtttagtaa ttttattcgt taattgaatt taatgtcttc caagaacatc
    17821 ataaggctca gcaccattat tgggattata tcctggctgg tctttgttac atctgatttg
    17881 tatattcttt ttgggcaaat caataacctg aaaattggga ttcccgccat tacaccccgc
    17941 tttgcattcg atttgtttat cctttccatg tttttctatt acaagcatca ggcagaaaaa
    18001 gttgaaaccc tcaacttcat tgacctgcta tggagagtat ttgcaaccgg tttgctgact
    18061 accgtggtta gtttggccat caagtttcta ttctttgctt ttggaaatac aaagcttgcc
    18121 gaagacgttt tcctgatcaa tcttacttac cacataaata ttggtttaat aggagctttt
    18181 ctggtttcta ctttaatagt ttgggaaaaa ttgatactgt atcagaaatc gaaactgctg
    18241 ctgaaatcgt ggcaggtttt tcaacttact ttgctggcca gcttatttat caatttattc
    18301 ccattccaat ccaattccgc tttgtctgta atttctctta cagtcttaat tgtcatggga
    18361 ttggtattgt ctgtaaacct gaaatgggta gcttatctga acttcaaaca aaagtggaag
    18421 agtattctcc ttatcctgtt ggttatattg tacctgggat tctttcttca aaaccttttc
    18481 caatatgcat ccggagacaa cctgagttta aatattcttg agaatacatt tactattgct
    18541 cttttttctt ttgtgttttc gtatgcctta ttcagcttgt tggtaatact atttaacctg
    18601 cccacctcat cagtatttga acagaaactt gaagaagtta taaactttca aaaactaagt
    18661 caatccattc aaacagaaca gagtgaagac caggtttatg acatccttct tcacagcgct
    18721 ataaacactg tcttcgccga tgcaggctgg atagaaattt ttgaaaaagg tgaagggtca
    18781 ccataccata tgcttaacat atccgagaat gaggttgaaa gcataaaaaa gaatataaag
    18841 aattcaaaga tcaaaggaat tcttgatacc gatacggaca aatcactttt aacctcaaag
    18901 ctgagctcag tgttaaagtc aacccggttt aaatccatcg ttgcatatcc ggttctggtc
    18961 cagggaaagc agatcggaac cctggcactt ttgaaggagg tgtccgaagg atttaaccgc
    19021 gaaatgacca atattatccg cgcatttgta aaccaggcgg gtatctctat tgaaaatttc
    19081 cggcttcttt acgaagccat tgagaatgaa cggtataaag aagagctaaa aatcgccaag
    19141 atggttcaga aaggtctgct ccccaaacag ctggacatga attcatcttt tgaaattgcc
    19201 gcattttctg aagccgcgga tgaagtgggg ggtgattatt acgatgcttt caccataaat
    19261 aaagaaaaag ttgctctgat catcggtgac gtttcaggta agggtacttc cgcagccttc
    19321 cacatgtcac aaatgaaagg agtatttaac agtctggcac aactagggtt gcctccaaaa
    19381 gattttcttt gtaatgcaaa tacggcatta agcaccagcc tggatcgatc tagttttgtt
    19441 acctgctcct attttattgt ggatatagcc gcgcgaaaac ttgaatttgc aagggcaggt
    19501 cattgcccaa ccctttattt cgattcacaa tctgaaaaat caattttctt cgaagacaag
    19561 ggtcttggat tgggcatcct tagaaatgat gaatttggaa aatttctcga ggtgcgcaat
    19621 atgcaattta actcaggcga tattctagtt ctatatacag atggaattac ggaggctaaa
    19681 aatatgaaga agcatgaata tggttttgaa agacttggca aacttctcca ggataatcat
    19741 tcatcttcgc caactgttat taaagacata attattaaag acctgtacaa tttctgcgga
    19801 caggaaagcc ttgatgatga ttatacaatg ctgattgtaa aatttaaatg attttgaacc
    19861 agaaacatac atatacgtat tattaaacaa tgataaattt aacacacaag atagaaggcg
    19921 cgatttatat aattagtgtg gtcggtgatg ttgatgccag ttcatccatt caattggata
    19981 atgcacttgg ccaggccctg gaggataatc aaactaaaat tctggttgat tgctcaaaac
    20041 taaattatat atcctctgca ggattagggg tttttatgtc ttatatacaa gatttcgaaa
    20101 acaaaaatat caaattggtg ctgtgcaatc ttaatgaaaa agtaattgga gtcttccgga
    20161 tattggggct ggaccaactc ctgaaaattg tagacacaat tgaagaggga aagaaactta
    20221 ttgatgaatt atagttataa agttccttgt caaaaagaaa agctaaagga catcaggtct
    20281 tttgtaagcg aggctttacg caagcatggt gttcctgaag ttcaaatcgc cactatggtg
    20341 ctggcagtgg atgaggtgtg tgccaacatt ataattcata cttacaagtg cgatcctgat
    20401 gagtatgtcg aaataattat caaagtggat aaaaaactta attgtgtttt tgaaatttcg
    20461 gatcaggggg ataagtttga tattactcaa taccaggagc cctccatggc agagataata
    20521 agaaataaaa gaaaaggcgg catcggtctt attctggtgc gaaaaattat ggatgacatt
    20581 gaacttgtgc agaaagatgg gcaccacgtg tgtcgtttaa ccaaaaagct tggcaaggag
    20641 tcctgattct cttcatcaat taccttattt ttgtttcctt ctaaaaccag tttaaataaa
    20701 atgggaaaaa ttatatttac agcctttttt gttgctctgg cttttgctgc acactcattt
    20761 cagtccggaa ttctttttag tattggcgag aatcaaacct cgaaagagga gtttgctttt
    20821 ttttataaga aaaacagcca gagtaatccg gaggcatttg cccgggcaga tattgaagac
    20881 tatctgcggc ttttcatcga ttttaaactt aaggttaagc aggcagaaga tctgggctat
    20941 gacactactg caaattttac cagggaattg aatatctacc tgggtcagct cactgaatca
    21001 ttcatgaatt caaatacttc ggaagaaaaa ctctacctgg aagcatatga aagaatgcaa
    21061 tttgacattg atgcatcgca tattttagta agactaagtg aaaatccaaa accatcagat
    21121 acgttgccgg cttatcaaaa ggccaggtca atttatgatc gggccaagtc cggggaaaat
    21181 ttcggtgagt tggctgttca atactctgag gaaccgggcg cagctatgag caaaggacat
    21241 cttggctatt tcaccgcttt tcaaatggta tatccttttg aaaccgctgc atactcaatc
    21301 cctgttaatg gtatttctac accagtgcga agtcaatttg gatatcatat tattaaagtt
    21361 aacaaccgcc ggcctgcaat gggtaccgtt accgttgcgc atattatgtt gaggttcaat
    21421 agggatatgc aatcgccaga ctcattggaa gtgaggcaaa aaattgatat ggtgtatgat
    21481 gatctgacga agggcgcatc ctgggactca gtctgtgtaa aatattctga agatataaac
    21541 agtaaggtta atggcggaag attgagaccc ttcggcatca gaggtttgaa tgttcctgaa
    21601 tttgaactgg cggcccacga aacccccgtg gctgaaatat cagatccatt taaaactcct
    21661 tttggctggc atgttttgaa agtaattggt aaaaatacta ttggtacttt tgaggaagag
    21721 cgcgccggta tagtcaggaa agtccaaaat gacgcaaggt cacggcttcc gaaagaagaa
    21781 tggttgtctc acctaaaaga gagatacaaa tttgaggaaa aaaatgatct cagtgaaaaa
    21841 gtaacatttc tttatgactc aacgcaatca ccggtcggtt cagttattct ttttacactg
    21901 gaagatcaga gctatagtca attggattta gaggcctacg caaaatcacg aaacatactt
    21961 gatggggccg gcgctttgct gtggtataat aaattcattg aagatgaact gagaaatcgg
    22021 gaggaaaaat ttctacttca gaataatttg gaatatagaa tgctcagaaa tgaatattat
    22081 cagggaatat tacttttttc cattatggaa gataaggttt ggaaaaaggc ggaacgggat
    22141 tccgttgggc tgcgcaatta ctataaaaat aattcagacc gattcctggt tgccggcaaa
    22201 aagggaattg ttatttctgt ggaacccgac tcactcactg atcaggtatt taacagcttg
    22261 ttactcaatt ttaaagattt acatgccaat tatgatatct caaccgttaa aaattggact
    22321 gatgctgtcc tgagttctca aagtaggcta actttgcggt ttgcagaaaa ggttttccag
    22381 gaaggaaatt ctgcattaga gaacattgat tccggcatca acctgattac gaacgatggt
    22441 acacaggcca tactggttct tgcggatgaa acagggaact gggttggtca attcgaagac
    22501 gttaaaggac tggctatttc tttctaccag gatatcctgg aagagaattg gataaacgaa
    22561 ttacacaaca attacaaagt aaaaattaat aagaaagttt taaagcaggt cattaatgaa
    22621 ttggaaagca ggtagattga taatgattat tctgttattt tcttcctttt ggagctgcga
    22681 gtttctcaag atgaaaaatt cggacgttgt cgaggaatca actcaatccg aacctattgc
    22741 cagagtactt gatcattact tatatcctga agacctggca ggaataatcg gtcccggcgt
    22801 aagcaaggaa gatagtgcaa atcttgtttt aaggtatatc aaaagttggg cgcgtaagca
    22861 actgctaata aaccaggcgg cagcaaacat tgattttaat gaagcagaac ttgagagaaa
    22921 aattctggat taccgctacg cgttaatggt ttatgagttt gaaaagcaat acgtaaatca
    22981 ggagattaat ctggaattaa gccctcagga gatcagagat tattacgaaa aaaataagga
    23041 caaatttgag ctcaagcaaa atatcatcag gggggtttat gttaaaatac caaaccaggc
    23101 accaagactg gtgcaggtaa agaattgggt gttatcaaac aagcaaaaag atctcgaaga
    23161 attgcgttcc tattgtttac gtttcgccac aaattattca cttgaagatt ccgtttggtt
    23221 aaacttcgac gaagtaataa gaggaactcc cctgactgcc attaccaaca aagtggaatt
    23281 cctgaggaaa aacaggtttg ttgaatccac tgatgatgaa tttaattatc tgatccggat
    23341 tgatgaatat cgaattacag atcagatttc tccccttact tttgttgaag accagattcg
    23401 aagtataatc attaataaga ggaaagtcga actgacaagg aatttggaag ataaagtatt
    23461 taatgaagca gtcaccaaaa aagattttga agtgtatgtt aaagaataaa ctttttttgt
    23521 tagttggact ttttgcaact cattcattga gttttgcaca gaccaatgga ctggtgatag
    23581 ataagattat tgccaaagta gacaatcata ttatcctgaa atcagagctc gaattgggtt
    23641 accttgatat gattacccgg ggaatctcag ttgacgacaa ttcaaaatgc ggagttctcg
    23701 aaggccttgt gttgaataaa cttttgcttg ccaaatcgga aattgattct atttccgtac
    23761 ttgattttga ggttgaacga agtcttgaca acaagcttcg atactttatt agccagattg
    23821 gttccgaaga aaaactggaa gagtactacg gaaaaagcat ggagcagttt aaggatgaac
    23881 ttcgcgatca ggaaaaggag caattattga tacaaagaat gtcggaatcc atcaccgaag
    23941 atgtaacaat aactcccgga gaagtaagga gatttttcaa caggatacca atagattcac
    24001 ttccattttt ctcaaaggaa gtggttgttg gggagattgt tatgtatcca aaagcagggc
    24061 aagagagaaa agatcaagcc aaagaactct taagagcctt gaaagaaagg atactttcgg
    24121 gcgaaagctt taaccaactt gcatctcaat attctcagga gccggctgca tcacgatccg
    24181 gtggagagct ggggtttttt cgcaggggag aacttgccca tgagtacgaa gcagctgcat
    24241 tatatctgca gccaggagaa atttcgattc cggtggaaac ggaattcggc attcacctaa
    24301 tacaactcct tgaaagaagg ggaaatgaat ataatagccg gcacatttta cgaatcccgg
    24361 caccatccca ggaagatatt caacgaacgg taaataagat ggatagcttg tggaaaatga
    24421 ttacgcttga tagcataaag tttgaaaagg cggcccggga tttttcagaa aacaagttaa
    24481 caaacattaa tggcggattc atgcaggatc aaaccgggtc agatagaata tcggttgagc
    24541 aattagatcc aaacaccttc tttacattgg atactatgca tgtaggatca atctcaccgc
    24601 caatagttta taaagatgaa gccggaaagg atgcggtcag attggtcttc tttaaaaatt
    24661 caatccgccc tcaccgggct aatcttaatg atgattggtc taaaatcaga attgctgcct
    24721 taaatgagaa aaagcagatt gttttagatg aatggttcga gaagtcccga ttcgaggttt
    24781 tcatcagaat agacgcggag tatgataatt gcaacatcat gaattaatga ccgttaaacc
    24841 gaagtataac agataatcaa tgaaggaaat tacaaacgaa gttggagctg cagacaaatt
    24901 gcatgaagca tatcaatcgc tgaaaaaaga gattggcaaa gtgattattg gacaagacga
    24961 agtagtacgg ctggtgttga cttccatctt ttgccaggga cactgtttgc tcgttggtgt
    25021 tcccggctta gctaaaacat tattgattca aacaatctca tccgctcttg atctgaaatt
    25081 cagtagaatt caatttaccc ctgatttaat gccttctgat attctcggtg cagaaacgtt
    25141 ggacaaagat cgcaattttc aatttatgaa agggccggtt ttcgccaaca taatcctggc
    25201 ggatgaaatc aatagaaccc cccctaaaac tcaggcagca ttattggaag ccatgcagga
    25261 gtattctgtt actattgccg gcaaaacctt ccatcttgaa agaccttttt tcgtattggc
    25321 tacacagaac ccgattgaac aggagggaac ttatccactt ccagaggctc aactggacag
    25381 gtttatgttc aatattctcc ttgattatcc ctcttatgaa caggaggttg aaattgtaaa
    25441 aggaacaacc actgactccg ggatttcggt ggggaaaata cttactcatg aagaaattct
    25501 gggctatcag aaattagtca ggatggtgcc agttccagac aacgtaattc aatacgcggt
    25561 ggatttggtt cacaagacca ggccattcac ttccagggga gaaaaattat ccgatgattt
    25621 cctggaatgg ggtgccggcc cccgggcatc tcaatatctt gtattaggcg ctaaagcaaa
    25681 tgctttactt aacggcaaat acagccctga tattgaggat atccaggcgg tggcaaagcc
    25741 tactttaaga catcgaatag t
//
LOCUS       DASWHX010000113         6248 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_97876, whole genome shotgun sequence.
ACCESSION   DASWHX010000113 DASWHX010000000
VERSION     DASWHX010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 6248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6248
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97876"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="VGA21_07945"
     CDS             <1..180
                     /locus_tag="VGA21_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009578160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650477.1"
                     /translation="TFYDEDGEVFDTTHFPGSARKFPFRGKGFYRIKGKVVEDFGYPM
                     IEVHFMEKLPMVTNQ"
     gene            complement(548..994)
                     /locus_tag="VGA21_07950"
     CDS             complement(548..994)
                     /locus_tag="VGA21_07950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650478.1"
                     /translation="MWYNLLTNKTDASSVTRNHGKSKAVTIRIRVSTGSADESSNQAI
                     FLSSFTDGQNSEKFQYYRPINNLYNPGYSLKKPLYITCQFIGDEFFGFVDELDIYAYG
                     NSEPEVISEIHEDISNILYLLHHPDGELGRKAKKIKKFLSSLVDIK"
     gene            complement(997..1341)
                     /locus_tag="VGA21_07955"
     CDS             complement(997..1341)
                     /locus_tag="VGA21_07955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650479.1"
                     /translation="MGQNGNPKKIEIKYVKTGGFRIYHIDGFFGGLTPQGKIYFEPYI
                     DKFHTPKSSTYPIEKDGSLGVELRDFRSSEKGGLREIEAGLILDINIAQSLVVWLQGK
                     IDEYNQLVKKTK"
     gene            1531..1665
                     /locus_tag="VGA21_07960"
     CDS             1531..1665
                     /locus_tag="VGA21_07960"
                     /inference="COORDINATES: protein motif:HMM:NF013561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraY domain-containing protein"
                     /protein_id="HEX9650480.1"
                     /translation="MKDESITIRIDAETLNRLRELAEKDQRTLSNYIRLVLTKHIEKV
                     "
     gene            1979..2278
                     /locus_tag="VGA21_07965"
     CDS             1979..2278
                     /locus_tag="VGA21_07965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650481.1"
                     /translation="MFSPLLIRIGSRIVLTGVFFLLFITLLIAQPGTGGNPGGGGQGG
                     NPGGGQGNPCVGPNPPPNCGQPAPIEGLYFLFLGGLAIGLYFTWQKHRNSKILKD"
     gene            2398..3300
                     /locus_tag="VGA21_07970"
     CDS             2398..3300
                     /locus_tag="VGA21_07970"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEX9650482.1"
                     /translation="MRKTGVVFILLSAISIEMALAQSFYSGSRDRAWMVSLGTGISSY
                     FGEVNNPKDKFDTKLNIEFGLKYMFAPRFGARLNVTWFRLQGDDATSKYDPIIERNLS
                     FRSDNLEVAVMGIAQLIPEGSRFYQRPRFNPYLFAGIGYLYFNPKAEAPAEYNGQPLP
                     DAGKWVPLQPLMTEGVDYKRSAFVIPYGVGVNIRISPFFNLVIDGGYRWTFTDYLDDA
                     STVHKDPASFSDPLAAALADRRPEIGKAPAEEGDIRGNPDRNDGYFLLNAKIEYFLPS
                     EFFQNIFGGGDKLRTIKVKRRRRR"
     gene            complement(3307..5220)
                     /locus_tag="VGA21_07975"
     CDS             complement(3307..5220)
                     /locus_tag="VGA21_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013455594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HEX9650483.1"
                     /translation="MQTRISAIRILPRWIIVLIDLGFILASIVLAYLLRFNFDLNPIA
                     SRNIFSEIFFYLSAHLIVIMVLKPYRGIIRYTGPQDAIRVVRAMLISFMLLLGVNEIY
                     NLTYGLRVIPFSVLSIAFFISVFTLVSYRILVKALFSYYSASVKKQVSILIFGAGKLG
                     QLTRQIIESDSYSNMKVLAFVDDDRQKTGNQIGSTPIIGLTDISKFAAYPRMELIIAL
                     RDISPMRKNEVVDACSIFKIKVRQVPSFDSWVKGELSVRQIRDVSIEDLLGRETISIN
                     NIKIGHELEDKRIFVTGAAGSIGSELVRQISQYNPSLMVLIDQSETGIFDLECEIESN
                     TTKIQYQFVVADITNPQRIEQLFLKYHPDVIFHAAAYKHVPIMEKNPSEAILCNVLGT
                     KYLADLSFKFMVKKFVMISTDKAVNPTNVMGASKRIAEMYVQSLNNFKVANKEYYTKF
                     VTTRFGNVLGSNGSVIPLFKKQIDKGGPITVTHPDITRYFMTISEAVELVLEAASMGE
                     GGEIYLFDMGESVKIVDLAKRMIHLSGLELNKDIEIHFTGLREGEKLYEELLNNKENT
                     ISTYHEKIMIAKVVEYAYMSISREIDKLIDLAKEKDEFEVVTQMKKMVPEYLSSFSRY
                     EILDKQGETGKAV"
     gene            complement(5221..5439)
                     /locus_tag="VGA21_07980"
     CDS             complement(5221..5439)
                     /locus_tag="VGA21_07980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650484.1"
                     /translation="MPAIKIKPFRVLRPLLILIGTTLVSLSIAVAQPGDPGGDPDNPV
                     PITGIELLIAAGAWLGIRKIRLLNKDKK"
     gene            complement(5466..>6248)
                     /locus_tag="VGA21_07985"
     CDS             complement(5466..>6248)
                     /locus_tag="VGA21_07985"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEX9650485.1"
                     /translation="IKVSGRNSVGCESQKTNISISQITATEPENLTTSFACGPENAVM
                     LEVGGAPDGFTYNWYESAEAITPVQSSESDKFLTEPLSSSKSFYVTMVSPEGCESLRT
                     LVMAEIFSINITASEDKLISSSDTGNQWFFNGNAIEGATDKEYAPEESGTYTLTVTRG
                     TCTLEESVDFLVTSIGENEAGLLTLYPNPVTNVLNVIVSNELIDEGGSKMLILDNSGR
                     IMLNSDIVSSETEVDLGSLSKGVYFIHISNKNQKIIRRIVKE"
BASE COUNT         1896 a         1203 c         1116 g         2033 t
ORIGIN      
        1 actttctatg atgaagatgg agaggttttc gatactactc attttcccgg atcggcgagg
       61 aaattccctt tccgcggaaa aggattttac agaatcaaag gaaaagtggt tgaagatttc
      121 ggttatccaa tgattgaagt ccatttcatg gaaaaattac caatggttac aaatcaataa
      181 tcatgtatat aatatattca ttatggcaat tatatatgag ttgaacccaa ttaaatgata
      241 aggaaggact ctatttctaa aattgtgtat ctcttagatg ctggtaatag tcatttccct
      301 ttacattaca atcaatgaga ccattataca tgtgggagtc aatgcataaa gcctgagcta
      361 attttttggc tttatgtata tgaaattcat ctttatggtt attgggaatc ttaaggtaag
      421 ggctaataat aacagagtca atgataattt gaccgattgt atgcttgcct ttgtttttta
      481 cagttggctt aactttcgct aagatttgtt ttctaataac tttctcagga tatactttaa
      541 aggccaccta tttaatgtca acaagagagg acaaaaattt ctttattttc ttagcctttc
      601 tacccaactc accatccggg tgatgaagta aatatagaat atttgaaata tcttcatgta
      661 tttcagatat cacctcaggc tcagaattac cataggcata aatatccaat tcatctacga
      721 agccaaagaa ttcatcgcca ataaattggc atgttatata tagcggcttt tttagtgaat
      781 agccgggatt atataaatta ttaattggcc tataatattg aaatttttct gaattttgtc
      841 cgtctgtaaa tgacgataaa aatattgctt gattgctaga ctcatcggca gaacctgttg
      901 atactcttat tcttatagtc acagccttag attttccatg attacgagta acgctagagg
      961 catcagtttt attagttaat agattgtacc acatgtttat tttgtctttt ttactaattg
     1021 attatactca tcaatctttc cttgtaacca cactaccagc gattgagcaa tatttatatc
     1081 taatattaag ccagcttcaa tttctctaag tccgcctttc tcggagcttc taaaatcgcg
     1141 taattccact ccaaggctgc catctttctc tattggataa gtggaggatt tgggagtatg
     1201 gaatttatct atgtaaggtt cgaaataaat cttgccctga ggtgttaaac ctccaaaaaa
     1261 accatcaata tgataaattc taaacccccc agtttttaca tatttaattt caatcttttt
     1321 tggattgccg ttctgaccca tatttcccgt tgaagctaaa aatcaaataa aaactgcgaa
     1381 gttagcccat aaatgtgatt attttttaat tcacttacaa atttaaacca ttaagtattg
     1441 gtatagtaac acttgttagt caaaaattgt tccgagaaaa tatcgggata catttggata
     1501 ttgtcttgaa atgtattact tttacataca atgaaagacg aatcaataac cataagaata
     1561 gacgctgaaa ctcttaatag gttgagggaa ttagctgaga aagaccagcg caccttgagc
     1621 aactacatcc ggttggtgct taccaaacac attgaaaaag tataaaatcg tgaaagctaa
     1681 atttgacaat ctctaccaat tagcccaaca ctatgcacat aacaacatta ctgggtaact
     1741 ttttttgtag ttttttgtag tcacatttta atggaactaa gaataactat aaatctgcca
     1801 gattgggtct attacaatat tatctagggt tgttcatatt attctcaaaa taagactaat
     1861 tttagattga attatgctag taaattgagt ataaattgta tataattata ttaattataa
     1921 gtataataat attagatttg taattaactg tatattatat atataacttt attttattat
     1981 gttttctccc cttcttatca ggattgggag tagaatagtt ctgaccggag tattttttct
     2041 gcttttcatt actttgctaa tagcccaacc agggacagga ggaaatccgg gtggtggcgg
     2101 acagggtgga aatcccggcg gtggacaggg aaacccatgt gtaggtccaa atcctccacc
     2161 aaattgcggt cagccggcac ctatcgaggg tttgtatttt ctatttcttg gtggcctggc
     2221 aattggcctg tattttacct ggcaaaaaca tcgaaactct aaaatcctta aagattaaaa
     2281 agataaatct aatttcttac cccgtagatt aattaccaaa ctaagagcca aaaaggctta
     2341 aaagaccgat taccctttct tttttttcgt aaattggcct gaggttagtt aagactaatg
     2401 agaaaaaccg gagttgtgtt tattctgctt tccgccattt ctatagaaat ggcattggca
     2461 caaagtttct attccggatc ccgcgatcgg gcctggatgg tctcccttgg aaccggcatc
     2521 agttcatatt ttggtgaggt taataatcct aaggataagt ttgatacgaa actcaatatt
     2581 gaatttggat taaaatacat gtttgcaccc aggttcgggg ccagattaaa tgtaacctgg
     2641 ttcaggttgc aaggtgatga cgcaacttca aaatatgatc caataattga aaggaactta
     2701 tcatttcgat cagacaatct tgaggtggca gttatgggaa ttgctcaact aattcctgaa
     2761 ggatcaagat tttaccaacg tcccagattc aacccatatt tgtttgctgg tattggttat
     2821 ttgtatttca atcccaaggc ggaagctcct gcagaatata acgggcagcc cctgcccgat
     2881 gccggcaaat gggtaccctt gcagccttta atgacagagg gagttgatta taaaagaagt
     2941 gcctttgtaa ttccttatgg agttggcgta aatatacgta ttagcccttt cttcaatctt
     3001 gtaattgatg gcggctatcg ctggacattt acggattatc ttgatgatgc cagcacggtg
     3061 cacaaagatc cggcttcctt ttcagatccc ctggcagcgg ccctggcaga cagacggcca
     3121 gaaataggaa aagcaccggc ggaggaagga gatattaggg gaaatcctga cagaaatgat
     3181 ggatattttc ttttgaatgc gaaaattgaa tattttttac cttctgaatt ttttcaaaat
     3241 atttttggtg ggggagataa gctgcgtacg ataaaagtaa agcgaagaag gcgaaggtag
     3301 ctttgcctaa accgcttttc ctgtctcgcc ctgtttgtcg agaatttcgt agcgggaaaa
     3361 actgctcagg tattccggga ccattttctt catctgggtg acgacctcaa attcatcctt
     3421 ttctttcgcc aaatcaatca gtttgtcaat ttccctggag atactcatat acgcgtattc
     3481 caccactttc gcaatcatga ttttctcatg atacgttgag atagtatttt ccttattatt
     3541 gaggagttct tcatataact tctccccttc cctgagtccg gtaaaatgaa tttcaatatc
     3601 cttattcaat tcaagacctg acagatgaat catcctcttg gcaaggtcaa ctattttcac
     3661 agactcaccc atatcgaata aataaatttc tccgccctct cccattgagg ctgcctcaag
     3721 aaccagttca actgcttcgg aaatagtcat gaagtaacgc gttatgtcag gatgggtaac
     3781 tgtaattggt ccacctttat caatttgttt tttgaaaagt ggaatcacag aaccattcga
     3841 gcccagcaca tttccaaaac gggtagtaac gaattttgta taatattctt tgttggctac
     3901 tttgaaatta ttcagtgact gcacatacat ttctgctatt cttttagaag cgcccataac
     3961 atttgtcggg ttcaccgctt tgtctgtaga aatcatgaca aacttcttca ccatgaattt
     4021 aaatgagaga tcagcaaggt attttgtccc cagtacatta caaagaatag cttccgatgg
     4081 atttttttcc ataataggaa catgtttgta ggctgctgca tggaaaataa cgtccggatg
     4141 atatttaaga aacaattgtt ctattcgttg aggattggtt atatccgcga ccacaaattg
     4201 gtattgaatt tttgttgtat tagattcaat ttcacattcc agatcaaaaa ttcccgtctc
     4261 agattgatca atcagtacca taagggacgg attatactgt gatatctgcc tgactaattc
     4321 acttccgatt gatccggcag ctcctgttac aaatattctt ttatcttcta attcatgtcc
     4381 aattttaata ttattaatgc tgatagtttc tctaccaagc agatcttcta tgctaacatc
     4441 ccgtatttgt cttacactaa gttcaccttt tacccaactg tcaaagctgg gtacctgacg
     4501 cactttaatt ttgaatatag aacatgcatc aacgacttca tttttccgca ttggagaaat
     4561 atctctcagc gcgatgatta gttccattct gggataagcc gcaaacttac ttatgtctgt
     4621 taatccgatt atgggagttg agccgatttg atttccggtt ttttgcctgt catcatcaac
     4681 aaaggccaac actttcatat tggagtaaga atcagactct attatttgcc tggtaagttg
     4741 gccaagcttt ccggccccaa agatcaaaat agatacctgt tttttaactg aagccgagta
     4801 gtaggaaaac agcgctttga ccagaattcg ataagaaacc aacgtgaata ccgaaatgaa
     4861 aaaagcaatt gaaagaactg aaaagggaat aactcttaat ccataggtaa gattatatat
     4921 ttcatttaca ccgagtagca acatgaatga tatcagcatt gccctgacaa ctctgatagc
     4981 atcctgggga ccggtataac ggataattcc ccgataaggt ttgagaacca ttattacaat
     5041 gaggtgagcg gacagataaa aaaatatctc actgaaaata tttctggaag caatgggatt
     5101 cagatcaaaa ttgaatctaa gcaaataggc gaggacaatt gaagccagaa taaaaccaag
     5161 gtctattagt actataatcc aacgagggag gattcttata gctgagattc tggtttgcat
     5221 ttatttttta tccttgttta gtaatcttat tttcctaata cccaaccagg ctccagccgc
     5281 aatcaatagc tcgataccag ttattgggac cggattatcc ggatctcctc caggatcacc
     5341 gggctgcgca accgctatag acaacgaaac caatgttgtt ccaattaaaa taagtaacgg
     5401 tcttaatacc cgaaagggtt taattttgat tgcaggcatt ctccacttgt ttcggttgat
     5461 tgggcttatt ctttaactat tcttcttata attttctgat ttttattact tatatgaatg
     5521 aaatatactc cttttgataa acttccaagg tctacttcag tttcacttga aacaatatcg
     5581 ctatttaaca taatccttcc agaattatca agaattaaca tcttactccc gccttcatca
     5641 attaactcat tacttactat cacattcaaa acgttggtca caggatttgg ataaagggta
     5701 agcaagccgg cttcattttc acctatcgaa gtaacaagaa aatcaactga ttcttccagc
     5761 gtacaagtcc ccctagttac tgttaaagta tatgttcctg attcttccgg agcatattcc
     5821 ttatccgttg caccttctat tgcatttcca ttaaaaaacc attgattccc ggtatcgctg
     5881 gaagaaatta gcttgtcctc acttgcagtt atatttatgc tgaaaatctc cgccatgacc
     5941 aatgttctta aactttcgca cccttccggg gaaaccatag ttacataaaa agattttgaa
     6001 gatgaaagag gttccgtcaa aaatttgtca gattccgatg attgaacggg agtaattgct
     6061 tctgctgatt cataccagtt ataagtaaac ccgtccggcg cacctccaac ttccaacatc
     6121 acggcattct ccggaccaca cgcaaaactt gtagtaagat tttcaggttc ggtagcggta
     6181 atttgactaa ttgaaatatt tgttttttga ctttcacatc ccactgaatt tcttcctgag
     6241 acctttat
//
LOCUS       DASWHX010000114         6157 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_1586865, whole genome shotgun sequence.
ACCESSION   DASWHX010000114 DASWHX010000000
VERSION     DASWHX010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 6157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6157
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1586865"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            202..1323
                     /locus_tag="VGA21_07990"
     CDS             202..1323
                     /locus_tag="VGA21_07990"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9650486.1"
                     /translation="MKHYSILLIFFLVEYSAAFSQTTARDWMDKGIELAEKENYKEAR
                     EALDKAIEVDPKIPKFYLERGKIRNQQEDFYGAIGDLNIAVELDSNYAEAYNERGLSK
                     FYLADDEYAIVDFSRAIEKKNDYAEAYSNRGFALFNLEDLENAYFDFSKAIELDPNDA
                     DKYYNRGVIRAELEEFIGALDDYNKSISLDASDPEVFNYRGVARYNLGDFAGAITDLN
                     QAISMDSTDAIKYDNRAMIKSALEDYQGAVSDYDKSVALFDEDPDVYNLRGVAKYNLE
                     DYEGAIQDYDLAIELDPEEPLFYDNRGLAKSEIEDYNGAIEDYTVSIELFPNDPEPYY
                     YRGMAKINLGNNYDGCLDLNRASQLGSSSAGKAIKEYCK"
     gene            1335..2429
                     /locus_tag="VGA21_07995"
     CDS             1335..2429
                     /locus_tag="VGA21_07995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650487.1"
                     /translation="MDLRIARISHLCTIFILGISFNLLAQTNNDNIEDRVEILEDTPI
                     QSFTNGNSVQWNCVDEKLTGKCIKYHNDQWFYFIALDAPVQYINISNQKCRDIWGVQL
                     VVIEGIPCQTETYQIKSCISLGTQDDMFVTLDNLEPGKEYLLNVDGYLHDFCHFKMEL
                     SLTPHGLPVVQTIQIEASSPVRQDTLNYFWITPDSLMQSVTSYALYRRKTNEFKFSLI
                     KMFPARQDAHGEIRKEYEYVEILTGENQPWSYKLIASIGNTGNTVDEFSAQGLSKVKT
                     RYKIYPEIDLKKNAKLEILLTDYVLGVTLENLSIEYFPRDFKPIYFDVTEYIDLGVTR
                     FQVVIKNLNTGKRAYLDFEYQKGIGVNKMK"
     gene            2547..3047
                     /locus_tag="VGA21_08000"
     CDS             2547..3047
                     /locus_tag="VGA21_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014020087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX9650488.1"
                     /translation="MALLISYTLIIAQTPEEKLAELGLKLADPEPPVANYVRAVRTGN
                     LVFLAGHGPVRPEGGYITGKVGKDLTQEEGYEAAKVVGLAILSSLKKEIGELSKVKRI
                     VKVLGMVNCTPEFGNQPEVINGFSDLMVKVFGEEKGKHARSAVGMMALPRNISVEIEM
                     IVEIEN"
     gene            3123..5012
                     /locus_tag="VGA21_08005"
     CDS             3123..5012
                     /locus_tag="VGA21_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002694751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEX9650489.1"
                     /translation="MKRLVFLVQLVLSMCFWACNIQNENTEEIMPLDPHSYAAPEDAM
                     VTHLSWKADVDFSQKKITAVATLKFKKSNNSKHLILDTKDLEIKAVYLDNAVTPCDFT
                     LQPKDEILGSALIIPIKENTGSVSIEYATTDHAEALQWLEPAQTAGGEHPFLFTQSQA
                     ILARSWIPLQDSPGIRFTYDAEVTVPGNLLAVMSASNPQVKNDSGVYHFEMKQPIPAY
                     LMALAVGDIAFKSVGPRTGVYSEPSILESAAYEFAEMEEMLTTTEAIYGPYQWERYDL
                     IVLPPSFPFGGMENPRLTFATPTVIAGDRSLTSLVAHELAHSWSGNLVTNATWNDFWL
                     NEGFTVYIENRIMEALNGKSYSDMLAGLALQDLQDEVESKIREGSQADTRLFLQLEGR
                     NPDEGVSSIAYDKGFFFLKFLELKIGRERFDAFLKQYFADYQFQSMTTEKFVKYVQQN
                     FKDNIKNTEIDQWIYGEGIPETLPAPESERFEAVDNVLKAWEGGSPITSLISPEDLIA
                     KTWSTHEWLHFIRNLPDEMNQETMRNLDEYLDFTYSGNSEILAAWFMHVIRNEYNPGY
                     ENLENFLVNVGRRKFLAPLYDELIKTEKGLEMARRIYSEARPNYHYVSTNTLDKMLNW
                     DESSM"
     gene            5166..5930
                     /locus_tag="VGA21_08010"
     CDS             5166..5930
                     /locus_tag="VGA21_08010"
                     /inference="COORDINATES: protein motif:HMM:NF014708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEX9650490.1"
                     /translation="MTTTIITEKEQAKGQFNFGEIRENKPIGFPQDGGKVKPYSNLFY
                     WAHAWTPGGESVIGEHPHQGFEIMSFVLKGSLEHYDSKHQGWRKLKEGDAQIIRSGNG
                     ITHAEKVNANSEFFQIWVDPDLDKTLNKPASYDDYQSKSFPVEEKNGFTIKHYHGSNA
                     PMQMDTPGVLIKEIWLNEGEHELNVKKEEVNSIYLIEGEIKVNENEMHAKDFAIVENG
                     EKISIVSRNNSRLFMLQTPATINYLTYAQRMRRHFA"
     gene            5935..>6157
                     /locus_tag="VGA21_08015"
     CDS             5935..>6157
                     /locus_tag="VGA21_08015"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEX9650491.1"
                     /translation="MTRVSEVIRRRKSIVLFDNKEVEQEKLDLIFESARWAPSSFNEQ
                     PWRFIYAHKSNPRDHGKIADALNDHNRSWA"
BASE COUNT         1980 a         1080 c         1354 g         1743 t
ORIGIN      
        1 aattatctga tttattatgc agaaaaattt gcaaacagct gggtataaaa atttttaagt
       61 tctctgatat gaaacgtctt ccatgctact catcatactc ttatggacat tctgatcagg
      121 tcgtcagacg gttccaaacc gtcggacgcc tctgcttttt acggttgaat tcaatttata
      181 cgaaatgatt acctttataa catgaaacat tattcaatcc tgcttatttt ttttctggtt
      241 gagtactccg cggcattttc acaaaccacc gcgcgtgatt ggatggacaa gggaatcgaa
      301 ctcgcagaaa aggaaaatta caaggaggct cgcgaagctc tggataaggc gattgaagta
      361 gacccgaaaa tcccaaagtt ttatcttgag cgcggaaaga ttcggaatca gcaggaggat
      421 ttctacggcg ccattggtga tttgaacata gccgtggagt tggattctaa ttatgcagaa
      481 gcgtacaacg agcgtggtct ttcaaaattt tatttagcgg acgatgaata tgcaatagtt
      541 gatttttcca gagcgattga aaaaaagaac gattacgcgg aagcatacag taaccggggg
      601 tttgctttgt ttaacctgga ggatcttgag aatgcctatt ttgatttttc caaagctata
      661 gaattagatc ctaacgatgc ggataaatat tacaaccggg gggtaataag agcggagttg
      721 gaagaattta ttggtgcact tgatgattac aataaatcaa taagccttga tgcatcagat
      781 ccggaagtat tcaattaccg tggggtggca agatataacc tgggtgattt tgccggagct
      841 ataacagatt tgaatcaggc tatctccatg gactccacag acgctatcaa gtacgataac
      901 cgggccatga ttaaaagtgc gcttgaggat tatcaaggag cagtttctga ctatgacaaa
      961 tccgtagctt tgtttgatga agatcctgat gtatataatc tgcgtggcgt agccaaatac
     1021 aatctggaag attatgaagg cgcgatccag gattatgatc ttgcaataga attggatcct
     1081 gaggaaccac ttttctatga taaccgggga cttgcaaaat cggaaataga agattacaac
     1141 ggagcgattg aggattatac ggtttcaatt gaattatttc ctaatgatcc tgaaccatac
     1201 tattaccgcg gaatggcaaa aatcaatttg ggaaataatt acgacggctg tctggatctg
     1261 aatcgtgcca gtcagttagg ttcctctagt gccggtaagg caattaaaga atactgcaaa
     1321 tagtacttcg atacatggat ttacggattg cccgcatttc tcatctttgc acgattttca
     1381 tattgggtat ttcattcaac cttcttgccc aaacgaataa tgataatatt gaggatcggg
     1441 tagaaattct tgaggacacc cctatccaat ctttcaccaa tggaaattct gttcaatgga
     1501 attgtgtgga tgaaaaactt accggcaaat gcatcaagta tcataatgac caatggtttt
     1561 atttcattgc cctggatgca ccggttcaat atatcaatat cagcaatcag aaatgccgtg
     1621 atatttgggg agttcagctt gtggtgattg aaggaattcc gtgccaaaca gaaacttacc
     1681 agattaagtc ctgtatttca ttaggtaccc aggatgatat gtttgttacc ctggataatc
     1741 tggaaccggg taaagagtat ttgctcaacg ttgatgggta tttgcacgat ttctgtcatt
     1801 ttaagatgga attgagcctc actcctcatg gacttcctgt tgttcagacc attcaaattg
     1861 aagcttccag ccccgtgcga caagatacat tgaattattt ctggataacc ccggattcac
     1921 tgatgcaatc cgttacttcc tatgcacttt atcgcaggaa aacaaatgaa ttcaaattca
     1981 gcttaattaa aatgtttcca gccaggcagg atgcacatgg tgaaataaga aaggagtatg
     2041 aatatgttga gattcttaca ggagagaatc aaccctggtc ctataaactg attgcttcca
     2101 taggcaatac cggtaatacg gttgatgaat tttccgcaca ggggcttagc aaagtaaaaa
     2161 cccgttataa gatttatccg gaaattgact taaagaaaaa tgcaaagctg gaaatattac
     2221 tgacagatta tgtactggga gttaccctgg aaaacctttc tatagaatat tttccaaggg
     2281 atttcaaacc gatttacttt gatgtaactg aatatattga tttaggggta acccgctttc
     2341 aggtggttat aaaaaatctg aataccggaa agcgtgcgta tcttgatttt gagtatcaaa
     2401 aagggattgg cgtgaataag atgaagtgac tccggaatta agggtcacag caaaaagttt
     2461 attttttaac agccgaatta ttagtatata ttgaataaaa ctcagtaaca atgaaaaatt
     2521 caacattaaa aaatgcgctg gccattatgg cattattaat ttcttatact ttgattatag
     2581 ctcagacacc ggaagaaaaa ttagctgaat taggactaaa gcttgcagat ccggaacctc
     2641 cggttgccaa ttacgtgcgc gccgtaagaa ccggaaacct ggtttttctg gcaggtcacg
     2701 gtccggttcg tcccgaagga ggatacatta ccggtaaagt aggaaaagat ctgacacaag
     2761 aagaaggtta tgaagcggca aaagtagtgg gattagctat attatcctca ctcaaaaagg
     2821 aaatcgggga actttccaaa gtaaaacgaa tagtaaaagt acttgggatg gtaaactgta
     2881 ctccggagtt tggaaatcag ccggaggtaa ttaatggatt ttcggatctt atggtgaagg
     2941 tttttggtga agaaaaagga aaacatgcaa gatctgccgt tggaatgatg gctttaccaa
     3001 gaaatatttc agtggaaatc gaaatgatag tggagattga aaattaagga agctgatctt
     3061 actgaccgga ttctctgcaa aataatttca cgttgcctta tttttgctct aaaagtgaat
     3121 atatgaaaag attagttttt ttagtccaac ttgttttatc aatgtgtttt tgggcttgta
     3181 atattcaaaa tgaaaatacc gaagaaatta tgccgcttga tcctcattct tatgcagcac
     3241 ctgaagacgc gatggttact catttgagtt ggaaagcaga cgtggatttt tctcaaaaga
     3301 aaataaccgc cgttgctacc ttgaaattca aaaaaagtaa taattcaaag catttgattt
     3361 tagacacaaa agacctggaa atcaaagctg tttatttgga taatgctgtt actccatgtg
     3421 atttcaccct tcaacctaaa gatgaaattc ttggctcagc attgataatt cctatcaaag
     3481 aaaataccgg gtcagtttcc attgaatacg cgactacgga ccacgcggaa gctttacaat
     3541 ggctggaacc ggcgcaaaca gcaggtggtg agcacccatt tctattcaca cagtctcagg
     3601 caatactggc acgaagctgg attccattgc aagattctcc agggatccgg tttacttacg
     3661 atgctgaggt aacagtacca ggcaacttgc tagctgtgat gagcgcctca aatcctcagg
     3721 taaaaaatga ttcaggcgtt tatcatttcg aaatgaagca gccaatcccc gcgtatttaa
     3781 tggcacttgc cgttggcgac atagccttca aatcggttgg accacgcaca ggggtatatt
     3841 ccgaaccttc aattttggaa agcgccgcgt atgaatttgc ggagatggaa gaaatgctga
     3901 caacaacaga agcgatctat ggtccttatc aatgggagag atatgacctt attgtacttc
     3961 caccaagttt ccctttcggg ggaatggaaa atcccaggtt aacatttgca actcccaccg
     4021 ttattgccgg tgatcgatcc ctcacttctc tcgttgctca cgagctggca cacagttggt
     4081 ctggtaacct ggttactaat gcaacctgga atgatttctg gcttaacgaa ggatttaccg
     4141 tttatattga aaacagaatc atggaggctt tgaatggcaa atcttattca gatatgctgg
     4201 cgggacttgc tttgcaggat ttgcaagatg aagtggaatc aaagattaga gaaggttcac
     4261 aagcagatac gcgtcttttc ttacagctgg agggtagaaa cccggatgaa ggagtcagtt
     4321 cgattgccta tgataagggt tttttctttc tgaaattttt agaattgaaa atcggacgag
     4381 aacgttttga tgccttccta aaacagtatt ttgctgacta tcaatttcaa tcaatgacta
     4441 ccgaaaagtt tgttaaatat gttcagcaaa attttaaaga taatattaaa aacaccgaaa
     4501 tagaccaatg gatttatggg gaaggaattc ccgaaacgct ccctgcgccg gagtcagaga
     4561 ggtttgaagc tgtagataat gttttgaaag catgggaagg tggcagtccg ataaccagtt
     4621 tgattagccc ggaagatttg atcgcaaaaa cctggtctac tcacgaatgg cttcatttta
     4681 tcagaaattt gccggatgaa atgaaccaag aaacgatgag gaacctggat gaatatcttg
     4741 attttaccta ttcaggaaat tctgaaatac ttgctgcctg gtttatgcat gtaattcgaa
     4801 atgaatataa ccccggatat gaaaaccttg aaaatttttt agtaaatgtt ggccggagaa
     4861 aattccttgc tccattgtat gacgaactaa ttaagacaga gaaaggattg gaaatggcaa
     4921 gaagaatata ttccgaagcg cgacccaatt atcattatgt ttctacaaat accctggata
     4981 aaatgttgaa ttgggacgag tcctccatgt gaaatgaaac ttaataaaaa taagaaggag
     5041 ggaatagtac tcatcattaa taccgcatta ttctaccagc caattcaact taaaaaaata
     5101 atttatatta atgttgtaac attaatgtta taacatcgta tacttgagta taaggagata
     5161 aaataatgac aacaacaata attactgaaa aggagcaagc caaagggcaa ttcaactttg
     5221 gtgaaattag ggagaataag cccataggat ttccacagga tggcggaaag gtaaaaccct
     5281 attcaaactt attttattgg gcgcatgcct ggacgcccgg aggtgaaagt gtcatcgggg
     5341 agcatcccca ccagggattt gagattatgt ctttcgtgtt gaaaggctca ctcgagcatt
     5401 atgacagtaa gcatcagggc tggagaaaac tcaaggaagg cgatgcccag attattcgtt
     5461 cagggaatgg gataacccat gcggagaaag tgaatgcaaa ttcagaattt tttcaaattt
     5521 gggttgatcc tgatctggat aaaacactga ataagcctgc atcttatgat gactatcaat
     5581 caaaatcctt tccggttgag gaaaaaaacg gatttacgat aaagcattat cacggtagca
     5641 acgctccaat gcaaatggat acgcccggag tgttaataaa agaaatttgg ttgaatgaag
     5701 gggaacatga attgaatgtt aaaaaagaag aggtaaattc aatttatttg attgaaggtg
     5761 aaattaaggt taatgaaaac gaaatgcacg caaaggattt tgcaatagtt gaaaatggtg
     5821 aaaagatttc aatagtttca aggaataaca gtcgtttgtt tatgttgcag actccggcta
     5881 ccataaatta tcttacatat gctcaaagaa tgaggaggca ttttgcataa tatcatgaca
     5941 agagtttcag aagtaatacg taggagaaaa agtattgtgc tctttgataa caaagaagtg
     6001 gagcaggaga aactggattt gatctttgaa tcagccaggt gggcgccatc gagttttaat
     6061 gagcagccct ggaggtttat ttatgctcat aaatctaatc cgcgggatca tggaaaaatt
     6121 gcggatgcat tgaatgatca taatcgttcc tgggcgg
//
LOCUS       DASWHX010000115         1953 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_2334444, whole genome shotgun sequence.
ACCESSION   DASWHX010000115 DASWHX010000000
VERSION     DASWHX010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 1953)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1953)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1953
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2334444"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..708
                     /locus_tag="VGA21_08020"
     CDS             <1..708
                     /locus_tag="VGA21_08020"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX9650492.1"
                     /translation="IRRQKKLSTIKNDFINNLTHEFKTPLASVLLASGILNKEYDQID
                     EEKRKNYLNLISHESKRLEGQVDKILQIAMIDSGNFTLEKKLLNVHDVINRVLESMMI
                     LVGERNGKIKLNLEASNPLVMADETHLVNIISNLVDNALKYTRLNPDIEITTSDREDG
                     VQITIRDNGIGIGKEIQKYVFDKFYRAQGGDVHNVKGFGLGLSYVKKIIEAHKGRIDL
                     NSSINQGTEFRLFFPLT"
     gene            714..1424
                     /locus_tag="VGA21_08025"
     CDS             714..1424
                     /locus_tag="VGA21_08025"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX9650493.1"
                     /translation="MSKILLAEDDESFGYILKEYLEMNGFEVTWTKDGNDGLKKFENG
                     LFDLCVLDIMMPEKDGFTLAKEIREIDSDVPFIFLTAKALKVDKLKGFRLGCDDYVVK
                     PVDEELFIARVRAIINRSSAQAGQNLSNCNTFWIGKYHFNPREQTLILDKKAAKLTTK
                     ETKVLKMLCQQKNQLVDRPKILKLVWGQNDFFNRKSMDVFIHKLRYKLKDDPAVKVTN
                     VHGKGFILEDPEEVKKAY"
     gene            complement(1513..>1953)
                     /locus_tag="VGA21_08030"
     CDS             complement(1513..>1953)
                     /locus_tag="VGA21_08030"
                     /inference="COORDINATES: protein motif:HMM:NF013698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX9650494.1"
                     /translation="PPDAGVLVKYLNKNFPGANYYAAYEAGYSGFWLQENLQAQGINC
                     IVVHAADVPTKDKERKQKRDPLDSQKIARSLKSGELEAIYIPSKDCQQDRSLLRARHQ
                     IVATKRDAETGSGITEFFWNQLPSAKSFNISVIKIKTCKPINDL"
BASE COUNT          615 a          343 c          429 g          566 t
ORIGIN      
        1 atcaggcgtc agaagaaact ttcaaccata aaaaatgatt tcattaataa cctgacccac
       61 gagtttaaaa ctccccttgc ttctgtactt ctggcttcag gtatactgaa caaagaatat
      121 gatcaaattg acgaggaaaa aagaaagaac taccttaatt tgattagcca tgaaagcaag
      181 cgactggaag gacaagtaga taagatattg cagattgcca tgatcgattc gggcaatttt
      241 actctggaga aaaagctttt aaatgtacat gatgtgataa accgcgtatt ggaaagcatg
      301 atgattcttg ttggggagcg caatggcaaa atcaaattaa atcttgaagc ctcaaatcca
      361 ttggtgatgg ccgatgaaac ccatcttgta aatatcattt caaacctggt agacaatgcg
      421 cttaaatata cccggcttaa tccggacatc gagattacta catccgaccg tgaggatggt
      481 gttcagatta cgataagaga taatgggatt ggcattggca aagaaattca gaaatatgta
      541 ttcgataagt tttaccgggc ccagggagga gatgtgcata atgtaaaagg atttggcctt
      601 ggattaagct acgtaaaaaa aatcattgag gcgcacaagg gccgcatcga cctcaatagc
      661 tcgatcaatc agggcactga atttcgattg ttttttccat tgacctaaag cgaatgagca
      721 aaattctcct cgctgaagac gatgaaagtt tcggatatat cctaaaggaa taccttgaaa
      781 tgaacgggtt cgaagtgacc tggacaaaag atgggaatga tgggcttaaa aaatttgaga
      841 atggcctttt tgatctttgt gttttggata ttatgatgcc ggaaaaagac ggatttacat
      901 tggcaaaaga aatccgggaa attgattctg atgtaccttt tatttttctc accgcgaagg
      961 cattgaaggt ggataagttg aaaggtttca ggctgggatg tgatgattat gtggtgaaac
     1021 cggttgacga agaattattt attgccaggg ttcgtgcaat tatcaatcgc agctcggctc
     1081 aggccggtca gaatttatca aattgcaaca ccttttggat tggtaaatac cattttaacc
     1141 ccagagaaca aacccttatt ctggataaga aggcagcaaa actcactacc aaagaaacaa
     1201 aagtgttgaa gatgctatgc caacaaaaga atcaattggt ggaccggcca aaaatactga
     1261 agttggtgtg gggacaaaat gattttttta atcgcaaaag tatggatgtt ttcattcata
     1321 agcttcgcta taaattgaaa gacgatcctg ccgttaaagt tactaatgtg catggaaaag
     1381 gatttatcct cgaagatcct gaagaagtta aaaaggctta ttagagatgt tggatgaagt
     1441 gtctaatcgc gacgggtgat tatggtttaa taagttttta actttgggaa ttcctcaaaa
     1501 tttacaaacc tgttagaggt cattaattgg tttgcaagtt ttaattttaa tgactgaaat
     1561 gttgaaactt tttgctgagg gtagctgatt ccaaaaaaac tcagtaatgc ctgatccggt
     1621 ttctgcatca cgtttggttg ccacaatctg atgacgagcc cgtaacaagc tgcggtcttg
     1681 ttggcaatcc ttgctgggta tataaatggc ttccagttct ccgcttttta atgaacgggc
     1741 tattttctgg ctatccaatg gatctcgttt ttgttttctc tccttgtctt tagtgggcac
     1801 atcagctgca tgtacaacga tacagttaat accttgggct tgtaagtttt cttgtaacca
     1861 aaatcctgaa tagccagcct cataggcagc ataataattt gcacccggaa aattcttgtt
     1921 caggtatttt accaacacac ccgcatcagg agg
//
LOCUS       DASWHX010000116        18882 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_3416044, whole genome shotgun sequence.
ACCESSION   DASWHX010000116 DASWHX010000000
VERSION     DASWHX010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 18882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18882
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3416044"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..556)
                     /locus_tag="VGA21_08035"
     CDS             complement(<1..556)
                     /locus_tag="VGA21_08035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650495.1"
                     /translation="MPSPTCASLSAVAEANSVNWPEIDVAAAGHYLYIVVGSCHLGEF
                     KYGCHCFDWCRGLRGNGFGAILGSYLSFLNLLSNIVVLKLLEIVNLVALNKVLMRETQ
                     KIIDKIESSEDCFCITLINGQKNPPNLFPTQIALDILPILYELLEGKGKVKKIALVIR
                     SNGGIIDAPLPVINLLKEYCETLSI"
     gene            complement(624..995)
                     /gene="crcB"
                     /locus_tag="VGA21_08040"
     CDS             complement(624..995)
                     /gene="crcB"
                     /locus_tag="VGA21_08040"
                     /inference="COORDINATES: protein motif:HMM:TIGR00494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEX9650496.1"
                     /translation="MKTLLFIGFGSFIGGILRYLLSSQIFTRFLSAFPYGTLAVNLIG
                     CFLIGLFFGFVEKTDVNPDLRIFLMTGILGGFTTFSAFSNETVGLLRQGSFGYASIYV
                     LSSLVLGILLTFAGIVLSRQL"
     gene            1276..1743
                     /locus_tag="VGA21_08045"
     CDS             1276..1743
                     /locus_tag="VGA21_08045"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9650497.1"
                     /translation="MAWPNPLIHIVDDDPVFTKFILSHIQSNGYDRVKTFESGEEFLG
                     SLKENPAVVLLDFSLKGLNGLDVIRAIKRQKPRVKIVVVTVVNDPKLEDECLRIGAAD
                     YLVKEDTRMEAMKARVLDILHETVIDERRKYVVGAFAVIALIVLIYMMSGLLD"
     gene            1785..1868
                     /locus_tag="VGA21_08050"
     tRNA            1785..1868
                     /locus_tag="VGA21_08050"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1819..1821,aa:Leu,seq:cag)
     gene            1961..2899
                     /locus_tag="VGA21_08055"
     CDS             1961..2899
                     /locus_tag="VGA21_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019987351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEX9650498.1"
                     /translation="MTNTFKAAVIQGSPALFDRKKTVKKMDHYIQSAAKRKPKLILFP
                     EAFIPAYPWGLTFGTKLGGRTPEGRRTWRKYVENAVEVPGPEVTAIGKMAAKAKAFIA
                     VGVIEKDTHSQGTVYCTLLYFGPDGKFLGKHRKLKPTAGERIIWGEGDGTTLTVLDTK
                     LGRIGGLICWENYMPLARMAMYSKGVQIYLAPTADHRESWVATMQHIACEGRCYVLGC
                     NQFVTKDMYPEDLEIGNDLKNSKKILCRGGSVIVDPLGNILAGPLWDKEGILYAEIAL
                     EKTTEAKMDFDVTGHYSRNDVFTYSVHGMPEIIRVK"
     gene            complement(3007..3489)
                     /locus_tag="VGA21_08060"
     CDS             complement(3007..3489)
                     /locus_tag="VGA21_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009054105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HEX9650499.1"
                     /translation="MDLEKFSIKSKGQAKENKRFLKRFKKFDSKKLDGQFLALHEEVF
                     AEIDCLTCANCCKTTSPVFYQNDIERASNALKMKPGDFVETYLKIDEDNDYVLKSAPC
                     PFLDSGNHCKIYEHRPKACREYPHTDRKKMIQLLDLTYRNTLVCPAVLEIVNRMKVKV
                     "
     gene            complement(3496..3831)
                     /locus_tag="VGA21_08065"
     CDS             complement(3496..3831)
                     /locus_tag="VGA21_08065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650500.1"
                     /translation="MKKILCLVIISVIFGCAPDPIPQDQQVYVGIWQGSNIFLEIDAD
                     GNASYAKVMEGASETVDSPVKKIGDGKIVIGYLFITKTLELTKPPFEEDGKWKMVIDG
                     VTLTKQTTL"
     gene            complement(4029..4403)
                     /locus_tag="VGA21_08070"
     CDS             complement(4029..4403)
                     /locus_tag="VGA21_08070"
                     /inference="COORDINATES: protein motif:HMM:NF015147.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LEA type 2 family protein"
                     /protein_id="HEX9650501.1"
                     /translation="MRKLEFKEIRDGKIIANADAVFNNPNKRGGKVKKVDISIFVNEK
                     EAAKVMRNSPFKVSPNSEFTIPLEMEITFEELAKNFLSNIFSREKEKNLPLHFEGYIW
                     VSVFGVTRKIPVKYDASLKLRF"
     gene            complement(4497..4748)
                     /locus_tag="VGA21_08075"
     CDS             complement(4497..4748)
                     /locus_tag="VGA21_08075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650502.1"
                     /translation="MTDYSAKDLKLIYWLLICLFTIELAQIALKYFNDYYFNRIAIGG
                     FNLLIVYFGYKIGINKIFVIGYGLISMILIVAAYFIIWN"
     gene            4784..5698
                     /locus_tag="VGA21_08080"
     CDS             4784..5698
                     /locus_tag="VGA21_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009582091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HEX9650503.1"
                     /translation="MRLKLVDYKNIISLIICVFGQQLLLGQNFENIAYRGGAGLLPEN
                     SIPAFLNALDSNATILEIDVVISKDGEVVVSHEPFISSEYCLTDEGKELTVSDEQKLI
                     IYSMDYSQVRKFDCGTKGRTDFEAQERVPVNKPLLGEVIRECERYLKNYTQYEVDYLI
                     ELRSSEEGDSKDHPLPAEFSDRVFEVIDAYLPLSRIIIQSFDFRILKYWNEKYPEVRL
                     SALVESIKSDETVLEKLGFHPHFYSPHYKLIRKRTIRDLHELGIKVYPWTVNEEKDME
                     KMMRWEVDGIITDYPNILNRLILESSTN"
     gene            5757..6761
                     /locus_tag="VGA21_08085"
     CDS             5757..6761
                     /locus_tag="VGA21_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018471872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine kinase"
                     /protein_id="HEX9650504.1"
                     /translation="MAEKVYDVYGIGNALVDMEYSVEDNFLLTNGVEKGVMTLVDQKR
                     QNRLVQALNHNDVKKQCGGSAANSIIAISQLGGSCFYSCKVASDELGKFYLKDLSREK
                     VDSNLVIGSLPDGITGKCLVLITPDAERTMNTFLGITSEYSAGDIYESAIKRSRYIYI
                     EGYLVANPDGRAAMRRGRELAQKYNVRTSLTFSDPSMTRHFKEGLMEVIGERVDLLFC
                     NEQEALIFTESSDLFEAREKLKNFANSFAITRGRQGAMVYDGHNFADIDPVPVKAVDT
                     NGAGDMFAGAFLYGITHGYNFVDAGRLASLASSRVVANFGPRMCGSETKALLAELQAG
                     "
     gene            7127..8095
                     /locus_tag="VGA21_08090"
     CDS             7127..8095
                     /locus_tag="VGA21_08090"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEX9650505.1"
                     /translation="MIAFLFNNLAGKIKIGTALGKIDPKYNSIFGDILKGPFPFGHEC
                     VAKVIEIGDGVKNIKIGDVVSVPFQISCGCCINCNNGFTSVCETTPVLSMFGFGKHLQ
                     FGGAMSDFIKIPFADAMLLKIPNHIDPIHLASLSDNIPDAYRHLKGIETNPNQKVLIV
                     AGQAKSVGIYALMIAKALGVAEIDYVDNSKERLEIAQKLQANNIFESFHQVTGKYDLV
                     IDTSSTSKGLETTLKSVRHFGTISSSGIYIKKTPLSLIEMYAKGVNFKIGLTNSRTDG
                     EIVLRLLEKIEIPFELATTKLESWENAESAFLIETTKVIVKRCLSP"
     gene            complement(8386..9720)
                     /locus_tag="VGA21_08095"
     CDS             complement(8386..9720)
                     /locus_tag="VGA21_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008202460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HEX9650506.1"
                     /translation="MSEQKFPHALVILFGFFAFSCILAFIVPQGQYERQPDSEGNERV
                     VQDSYQTVATERITVKEILISIPEGIAGRADVIVLVFLLGGCFYVVEKTGALREGITF
                     LAGKVTGKEEIALVVVAVIFATGGALAGLQEEIIAMTPVLILLCRKLGYNTFVAVSVS
                     FGAAIIGGAFSPMNPFAVLIAQKISGVPLLSGSGFRLVVMLAALGLWVLMIIRYGNRN
                     RIQKEKSESGSTSMSGRGMVIFGMLILAFSYLAYGMVKLEWGFYEMSALFFVVGVVAG
                     LIGHLGVNGTAIAYVEGFREMTFAAILIGVANSIPLILQKGIIIDSIVYGIFTPLQFL
                     PAGLSAITMMFSQLLLHYPIPSYSGQAILTMPILSPLSDLIGLSRQVCVLAYQYGAVM
                     MDLIVPTNGALMAVLAIAGISYDNWFRFIWKPMLIILTLAAVALVVAVMTGV"
     gene            complement(9811..10569)
                     /locus_tag="VGA21_08100"
     CDS             complement(9811..10569)
                     /locus_tag="VGA21_08100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650507.1"
                     /translation="MKRIIPALLLFEVFTNSLLAQSRFERTIPYQPGKTLTMEFTYPE
                     IIKVQVHDLNEIKVEGIVSINSGLFDQNFDLVVNSSGNNIDIYSTIKDKDEIPHRITV
                     NVKGEKYVFMAASWNDPKIKEFLDQHDKSAINWISNGLDIDIELTVTVPRNSNLNITS
                     KFGTIEILGLPEQLVATSKHDGVDISVDPSSRYDFEIHTTFGEVYTNLDLELESQDGL
                     RPYKAQKLVAHLNGGGKKIVLESKFGNVYLRKKQ"
     gene            complement(10620..11423)
                     /locus_tag="VGA21_08105"
     CDS             complement(10620..11423)
                     /locus_tag="VGA21_08105"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HEX9650508.1"
                     /translation="MKTLKIKEMKKQIWTIAVALIFAAFSMPSFGQEYKIPASGKSLK
                     INFNEINKLEIEGTTGSEIIITTTDRSRAKDERATGLKAISSMGLEDNTGLGLSAVEK
                     NGVVEVNSISRNSESRYIVKVPKNVSVKYAHNSPYGSDLKISNLESELDVSSLHNDVL
                     LLNVTGPMTINTVHGDIDADFTTLNQASPTSIASVHGHVDVSVPASSKAELTIKTTWG
                     EIFTDMDIQMDTSADMKRISSQTVRGKLNGGGVALMLSSTHDNIYLRKK"
     gene            complement(11448..12236)
                     /locus_tag="VGA21_08110"
     CDS             complement(11448..12236)
                     /locus_tag="VGA21_08110"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX9650509.1"
                     /translation="MKEFDKEKMHDLLIEHSEGNLTGELREYVERYIENNTDARKEFG
                     ELKKLNRVMNQSREFEPDATLHTEFDKMLQEEMKLAASKSAKRGKVISMPMIWTMRVA
                     AGLAILILGYFLGTQFSTDKDELASLREEMEATRQLVMMALQNQSPSQRIMGVNYVED
                     MKQPDDQIMDVLIETLNEDENVNVRLAALNALTKLSSSEKVKLALIESLGEQTEPIVL
                     ISLIDALVNINEIRAVEKLQDLIDDDKNIQSVQDQAQYGLFKLM"
     gene            complement(12239..12796)
                     /locus_tag="VGA21_08115"
     CDS             complement(12239..12796)
                     /locus_tag="VGA21_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009582571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX9650510.1"
                     /translation="MTETNDEFLMEQVMQGDLQKASALFESYNKKLYSFFVRLTFDKD
                     LSHDLTQNVFLRMLKYRKSYRKGASFRPWIYQIARNVLADHFRKSDGKWMSDFDEVEK
                     MGIQTFDEHKSEEHQEQEKILHRSLARLPDDQREVLVLSRFQEMKYEEIAAITNTTVS
                     NIKIKVHRAIKKLRQTYFEIEKQME"
     gene            complement(12893..13318)
                     /locus_tag="VGA21_08120"
     CDS             complement(12893..13318)
                     /locus_tag="VGA21_08120"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX9650511.1"
                     /translation="MRRILFLTGLLITTTFGFAQETEEVQHKEIIKSIYFGGGSWYID
                     QQQMEELKEFVKSLENLSIYTITVISHTDNIGGIEYNNWLSTQRSKAVVRELLLNEVG
                     LEQIITDDRGQHTPLYDNMLPQGRMMNRRVDIKFTPLIL"
     gene            13450..14370
                     /locus_tag="VGA21_08125"
     CDS             13450..14370
                     /locus_tag="VGA21_08125"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9650512.1"
                     /translation="MSVLIIAPQRDMKEWKKAIQNVDASLHIEIYPELNNPESVKAAV
                     LWNHPPGILHNFPNLKWVTSLGAGVDHILKDKKIHSDIRITRIVDPGIAIQMSRMIVT
                     MLTFFEKDLLLNWNPGKTGGWNTISPVPELKIGLMGIGEIGKKVASVLTELKYEVVAF
                     GNTSRVYKNLRIFGQNELRQFLKSVDVAICLLPLTSKTENFINKDFFSLMNKGSYIMN
                     LARGKIVDEDALLWALKTGILKKAYLDVFRQEPLPATHPFWSNPEIIITPHISGITDV
                     MEGARQIVNNYHTFEDSEFLINEINRLKEY"
     gene            14372..14836
                     /locus_tag="VGA21_08130"
     CDS             14372..14836
                     /locus_tag="VGA21_08130"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX9650513.1"
                     /translation="MKDEFDRLVKLTGAVYDGEPWYGPSVKSILSDLTEDHRVLRTGE
                     SHSINELIFHMVAWRNMIAQTLEGNNEYVVSDEENFKPHANKYSLKEATEALEMSQVL
                     LLKALSTFDADKLQEKVPGKKYSYYQLIQGIIHHDVYHAGQISLLKKKIQSN"
     gene            14858..16462
                     /locus_tag="VGA21_08135"
     CDS             14858..16462
                     /locus_tag="VGA21_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013454359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX9650514.1"
                     /translation="MKKSITLFASLFISLTTFSQVLKAPPRAEGDGPWDQLIIRGVIL
                     VDGTGAPPFGPVDIVVEKNIIKQIATVGSPGVEINPNRRPQLKPGGKEIDATGKYLLP
                     GFVDMHGHIGGSAQGANAEYVFKLWMGHGVTTIRDPSAGNGLDWVLEEKDKSKKNQIT
                     APRIMAYTSFGSGAKEPVTTPQQAREWVRQNAANGADGIKFFGAPPEIMAAALDENKK
                     LGLRSACHHAQLNVAWWNVVNSARAGLTSMEHWYGLPEALFTDRAIQNYPLDYNYQNE
                     QDRFSEAGRLWKQAAPPYSEHWNNVMDELISVDFTLDPTFNIYEASRDLHRARRQEWH
                     EEFTMPKLWEFYQPSMISHGSYWHYWGTEEEVDWKQNYKLWMTFVNEYKNRGGRVTTG
                     SDSGFIFQLYGFAYIRELELLREAGFHPLEVIRAATLKGAEALGLDKEIGTIQVGKLA
                     DMIIVDENPLKNLKVLYGTGAIKLNENNEVIRVGGVRYTVKDGIVYDAKQLLEDVKKM
                     VAEEKAKTGFEIYQPGMKKINTNREN"
     gene            16452..18158
                     /locus_tag="VGA21_08140"
     CDS             16452..18158
                     /locus_tag="VGA21_08140"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEX9650515.1"
                     /translation="MRIRSWVGFAFLCIPFRAEAQSIDEYFDLARAEFRGENAFESVA
                     YIEQFWRLAGNSGFDKSIYHLEEKLKQAGFEKEGAANGRLTYRIEKRKLEEPSWEPIS
                     ASLTLEGSSSPLLDFATNRNMISINSHSTKNEGIEGEVVQLADGRIPAGVVVKGKILY
                     GEISPFRAYTLAAESGAIGIITYGNPSYLKPEINTRSIQFRSIPYNAGQKLWSICLSF
                     EANEKLKASLMSGKIKVRVKIETAFPVSDELTLVAEIKGSELPEERFVLSAHLQEPGA
                     NDNASGAGALMEMARVSATWINEKKIDSRRTITFLWGDEIVSTRNFVSEDSARRKNIK
                     WGLSLDMVGENTSLTGGRFLIEKMPDPSAIWTRGNDQHTEWGAGDVKEEDFFPHYFND
                     FVLEVFRRQAEFANWNVSYNPFEGGSDHVPFLQAGIPSVLLWHFTDQFYHTDLDRIDK
                     VSKQELANVGNGALVVLFTLVNADANTAGFLINQTFKAGKARLSAEADLSKKAIQLGR
                     SKQEELLIIDAWIRWYLNAIHSAKDIASSDAGFTQINHLANQKAAEFQQFARSLMSQF
                     ND"
     gene            18219..18536
                     /locus_tag="VGA21_08145"
     CDS             18219..18536
                     /locus_tag="VGA21_08145"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX9650516.1"
                     /translation="MPNIEHSFKALADSTRREIVYMLAVTPSGLSMREISDQFPASRQ
                     GVAKHLGVLVSAGLIQIQSAGREKKCLLNPLPLREVMDWLNFFKSYWEDRLNELKAHG
                     ESD"
     gene            complement(18573..>18882)
                     /locus_tag="VGA21_08150"
     CDS             complement(18573..>18882)
                     /locus_tag="VGA21_08150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650517.1"
                     /translation="EDDKFKVDLMTSIFYEPLTFEKCYERAKVQKLESFNLPIISLKD
                     LIEIKSNVKRYDGSLKDVVDAQELRKILERGEAKTINKQQSLFKRIFKKKGNDKGMSM
                     "
BASE COUNT         5726 a         3709 c         3898 g         5549 t
ORIGIN      
        1 aaatactaag ggtctcacaa tattccttca gtaaatttat caccggtaga ggtgcatcaa
       61 ttatcccgcc attactcctg atgacaaggg cgattttctt tactttccct ttaccctcaa
      121 gtagttcata taaaataggc agaatatcaa gagcaatctg agtcggaaat aaatttggcg
      181 gattcttctg accattgata agcgtaatgc aaaagcaatc ttcagaagac tcaattttat
      241 ctattatttt ctgcgtttct ctcattaaaa ccttattaag ggctaccaaa ttaacgattt
      301 ccagtagttt taaaacgaca atattggaca aaagattcaa aaaggacaga taactgccca
      361 aaatcgcacc aaagccattg ccgcgcaacc ccctacacca atcaaagcaa tggcatccgt
      421 acttaaattc tccgaggtgg cacgatccca caacaatgta taagtaatgt ccggcagcag
      481 ctacgtcaat ttcaggccaa ttaactgaat tagcttcggc aacggctgat agtgacgcac
      541 aagtcgggct tggcattcgt tctgaattta ccaaacccga cttatctggc gacatcttaa
      601 acacaaacgt taggtaatgc agtttataat tgccttgaca gcactatgcc tgcaaaagtc
      661 agcaatatac caagtacgag gcttgaaaga acataaatgc ttgcatagcc aaaactcccc
      721 tgccttaaca atccaacggt ttcatttgaa aatgcgctga aggtagtaaa tccacctaat
      781 ataccggtca tgagaaaaat tctcaaatcg ggattgacat cggtcttttc gacaaatcca
      841 aaaaagagtc ctataagaaa acatccgatt aaatttactg ccagggtacc atatggaaat
      901 gcagacagga aacgtgtgaa aatctggctt gataaaagat accggagaat accaccaata
      961 aaacttccga agccaataaa taaaagtgtt ttcattctta ataattttat tccggatacc
     1021 gtatttcttc tatatataaa attaccgctt gggggcgggt gtgaaaaaga gattgtgaga
     1081 gatacggcaa agttaagaat cattcccaag gtgccaattc attccgaaaa aattccaaac
     1141 catcagatct tttcaaaaca gaacaattta gttagaaggg aaattattag cgggattcga
     1201 atattgttgg taaaaacaga tatatttctc aaattcatgg tggctaaacc ctgaaaatct
     1261 gagacctaaa acccaatggc ctggcccaat cctttaatac atattgtgga tgatgatccg
     1321 gtattcacga aatttatttt gagtcatatt cagtccaacg gatatgaccg cgtaaaaact
     1381 tttgaaagtg gagaagaatt cttaggatct ttaaaggaaa atcctgctgt ggtattactt
     1441 gactttagcc tgaaagggtt gaatgggttg gatgtaatta gagcgatcaa aaggcaaaaa
     1501 cccagagtaa aaatcgtagt agtcaccgtg gtaaatgatc cgaaattaga agatgagtgt
     1561 ttaagaatag gtgcggccga ttaccttgtt aaagaggata cgaggatgga agccatgaag
     1621 gccagagttt tggatatcct gcatgaaaca gttatcgacg aaaggaggaa atacgtagtt
     1681 ggtgcttttg cagtaattgc attgattgtg ctgatttata tgatgtcagg attactggat
     1741 tgagtatcct ctctttggat taaattacta tttttgtcct ccctgccgaa gtggtgaaat
     1801 tggtagacac gcatgtttca gggacatgtg cccgcaaggg cgtgggggtt cgagtccctt
     1861 cttcggcact tttcattttt gatgcttatt ctatcagggg ttatcttttt ctccaataat
     1921 tcgcggtgag tttttgtaaa ttgaaccaaa atgatgaagc atgaccaata cctttaaagc
     1981 cgctgttatt cagggttccc cagccctctt tgataggaaa aagaccgtaa aaaaaatgga
     2041 ccattacatt cagtccgctg ctaagagaaa accaaaattg atcttgtttc ccgaggcgtt
     2101 tattccggca tatccgtggg gattgacttt tggaacaaaa ctgggcggca gaactccgga
     2161 aggcagacga acctggcgga aatatgttga gaatgccgta gaagtacctg gcccggaggt
     2221 aacggcgatt ggaaaaatgg ccgctaaagc aaaagcattt attgcagttg gggttattga
     2281 aaaagacacg cacagccagg gaactgttta ttgcacactt ttatacttcg ggccagatgg
     2341 aaaatttctt gggaaacacc gaaagctaaa accaacggca ggagaacgta taatctgggg
     2401 tgaaggggat ggaacaacgt taactgtttt ggataccaaa ctggggcgga ttggaggatt
     2461 gatttgctgg gagaactata tgccgcttgc acggatggca atgtacagca agggtgttca
     2521 gatttatctt gctcctaccg ctgaccaccg ggaatcctgg gtggccacaa tgcagcacat
     2581 tgcgtgcgag ggtcgctgtt atgtcctggg atgcaatcag tttgtaacca aagatatgta
     2641 tcccgaagat ttggagattg gtaacgacct gaaaaattca aagaaaatac tttgtcgcgg
     2701 tggaagtgtg attgtagatc cattggggaa tattctggca ggtccgctgt gggataagga
     2761 gggaatcttg tatgcggaaa tagcgctgga gaaaacaacc gaagccaaaa tggattttga
     2821 tgtgaccggc cattattcca ggaatgatgt atttacttac agcgtccatg gaatgcccga
     2881 aataatcaga gtgaagtaaa aattccagta cattttatga acattgttac tgactttttt
     2941 ttgtgaaaaa cggagatagt caactttctt ttgacgctca tacaagttat tcaatccccg
     3001 gatggatcaa actttgacct tcatcctgtt cactatttcc aaaactgcag ggcacaccag
     3061 cgtatttcga taggtaaggt caagcagttg aatcattttt tttctgtcag tatgtggata
     3121 ttcccggcaa gccttgggcc ggtgttcata gattttgcaa tgattgccac tatccaggaa
     3181 agggcagggg gcagacttca acacataatc gttatcctca tcgattttca gataagtttc
     3241 cacaaaatca ccaggcttca ttttcaaagc gtttgatgca cgctcaatat cgttttggta
     3301 aaaaacagga ctggttgttt tgcagcaatt tgcgcaggtg aggcaatcaa tttctgcaaa
     3361 aacctcttca tgaagagcaa gaaattggcc gtctaatttt tttgaatcaa attttttaaa
     3421 tctcttaaga aatcgtttat tctcctttgc ttgccccttt gattttatac tgaacttttc
     3481 aaggtccatc acaatttata atgtcgtttg tttggtgaga gtaacaccat caattaccat
     3541 cttccactta ccatcttctt caaagggggg ttttgtaagt tcgagtgtct ttgtaatgaa
     3601 gagatatcca attacaattt tgccatcacc aattttcttt acaggagaat caaccgtttc
     3661 gctggcgcct tccataactt ttgcatagga agcatttcca tcggcatcaa tttcaagaaa
     3721 aatattactg ccctgccata taccgacata tacctgctga tcctgcggaa tcggatcggg
     3781 tgcgcaaccg aaaataaccg aaatgataac caagcacaat atttttttca taccattctg
     3841 atttcactta taagttacaa catcttaact tcaattgcct tcttattata cgcgattatt
     3901 tttggataga aaatttgaat aaaaaattaa atttattgaa tgggatattg aacttatatc
     3961 acgcaaaaga aaagattccg gaatcaacaa aattaatttc gctcctgaaa gcttgaattt
     4021 tgttcagtct aaaatcgtaa tttcaatgat gcatcatatt ttaccgggat ttttcttgtt
     4081 actccaaaga ccgaaaccca aatatatccc tcaaaatgca gcggaagatt tttttccttt
     4141 tccctggaaa agatattgct cagaaaattc ttagcaagct cttcaaacgt tatttccatt
     4201 tccagcggaa ttgtaaattc tgagttcgga gaaactttaa acggagaatt tcgcattact
     4261 ttagctgcct ccttttcatt cacaaatatt gagatatcca cctttttcac tttgccccca
     4321 cgcttattcg ggttgttaaa aacagcatca gcatttgcaa taatttttcc atccctgatt
     4381 tccttaaatt caagttttct caccctgacg aattcaatat cctttacttc caagcaagaa
     4441 gagaagaaaa gacaaagcac tgcaaggaaa acaaatttta ttaaaagatt cgccatctaa
     4501 ttccaaatga taaaataggc ggccacaatc aaaatcatac ttatcaggcc ataaccgatc
     4561 acaaatattt tgttgattcc tattttataa ccaaagtaaa ctatcaacaa attgaagccg
     4621 ccaatcgcta tcctgttaaa gtaataatcg ttgaaatact tgagtgcgat ctgagcaagt
     4681 tcaatggtaa acaggcaaat aaggagccaa taaattaatt tcaggtcttt tgcggaatag
     4741 tcagtcatgt tagagtaagg tttttgttat cttgcaaatt gaaatgaggt taaaattagt
     4801 tgattataag aatattatta gtctgataat ttgtgttttc ggacaacagc tgcttctagg
     4861 ccagaatttt gaaaatatcg cctatcgggg cggtgctggt cttttaccgg aaaattcaat
     4921 acctgcattt ttgaatgcgc tggacagcaa tgccacaatt cttgaaattg acgtagtaat
     4981 ttctaaagat ggggaagtgg tggtatcgca tgagcctttt atttcctctg aatattgctt
     5041 aactgatgaa gggaaagaac tcaccgtttc agatgagcag aaactgataa tttattcaat
     5101 ggactatagt caagttcgaa aattcgattg tggcacaaaa ggcagaactg attttgaagc
     5161 tcaggaaaga gttcctgtaa ataaaccctt gctcggtgaa gtgatcaggg aatgcgaaag
     5221 atatctgaaa aactacacac aatacgaggt agattatctg atagaattaa gatcttctga
     5281 agagggagat tcaaaagacc atcctctgcc ggctgaattt tcggatcggg tttttgaagt
     5341 aattgacgcg tatctgccct taagcaggat tataattcaa agctttgatt tcagaattct
     5401 gaaatactgg aatgagaaat atccggaggt gcggctttca gcgttagttg aaagtattaa
     5461 atccgatgaa acagtgttag agaagctagg cttccaccct catttttaca gtccgcatta
     5521 taaattgata agaaaacgaa caataagaga tttacatgaa ttgggaatta aggtttatcc
     5581 atggacggtt aatgaagaaa aagacatgga gaaaatgatg agatgggaag ttgatggcat
     5641 aattacagat tatccaaata ttctgaatcg attaatactg gaatcttcaa caaattagat
     5701 tttattattt tcacatctgt gatgaaatga tttaatttcg agtaaaataa tgaaagatgg
     5761 ctgaaaaggt ttacgacgtt tatggaatcg gtaatgcact ggtagatatg gagtattctg
     5821 ttgaagataa ttttctttta acaaatggag ttgagaaggg agttatgacg ctggtggatc
     5881 aaaaacgtca aaaccgcctt gtgcaagcct tgaatcataa cgacgtgaaa aagcaatgtg
     5941 ggggatcggc ggcgaattca ataattgcaa tcagtcagct tggcggatca tgcttctatt
     6001 cctgcaaggt tgcaagtgat gaacttggga agttttacct gaaggacctt tcgcgtgaaa
     6061 aagtagacag caatcttgtt attggaagtt taccggatgg aatcaccggt aaatgtttag
     6121 tgcttataac tcctgacgct gaacgcacaa tgaatacctt tttagggatc acatcagaat
     6181 attcagccgg cgatatttac gaaagtgcaa taaagagatc caggtacatt tatattgaag
     6241 gttacctggt tgccaatcct gatggaagag ccgcgatgag gcgtggccgt gaacttgcac
     6301 agaagtataa tgtgagaact tcactgactt tttctgatcc aagtatgacc cgccatttca
     6361 aagaaggact tatggaggtg ataggagaac gagtcgattt acttttttgt aacgaacagg
     6421 aggcgctgat ttttactgaa agttctgatc ttttcgaagc tcgtgaaaaa ctaaagaatt
     6481 ttgcgaattc atttgctatt actcgtggca ggcagggggc aatggtttat gatggacata
     6541 attttgcaga tatagacccg gtaccggtaa aagctgtaga cacgaatgga gcaggtgaca
     6601 tgtttgccgg agcttttctt tacggaatta ctcacggtta taattttgtc gatgcgggga
     6661 ggcttgccag tttagcttcc tccagggttg tagccaattt cggtccgaga atgtgtggat
     6721 cggaaaccaa agcgctgctt gcagaactgc aggctgggta aaattttatc cgggaaagga
     6781 caaattctcc taatattcta atgccagatt taaataggac tagttccacg tataggtcct
     6841 tgaccttgac agtgacatag ttcacctctg ctttcgttac cttaattttt cctcaaaaaa
     6901 tttacggctt tggaaattta ccagtaaact atattgcaat gctaaaggac aaagacacgt
     6961 tttgaaaacc gcagccgtca caaacacaaa acgttacaca caattatgca gcagctatat
     7021 ttcattaaaa agggaaaact tgaatggcgt gaagtatcca ctcccgtgat ttcttcttca
     7081 ttggaagcaa tagtaagacc gtttgctgtt gcaaaatgtg accttgatga tagcattttt
     7141 attcaataac ttagcaggta aaattaaaat tggaaccgcc ttaggaaaga ttgaccctaa
     7201 atacaattca atttttggag atattttaaa aggtccattt ccttttggac acgaatgtgt
     7261 agcaaaagta attgaaattg gtgatggtgt taaaaacata aaaattggcg acgtagttag
     7321 cgttcctttt caaatatctt gtggttgctg tattaattgt aacaatggat ttacaagtgt
     7381 ttgtgaaaca actccagttc tttcaatgtt tggattcgga aaacatttac agtttggtgg
     7441 agcaatgtca gattttataa aaattccttt tgcggatgcc atgcttttaa aaattcccaa
     7501 tcatattgac cctattcatt tggcaagtct gtctgataac attccagatg cgtatcggca
     7561 tttaaaaggt attgaaacta acccaaatca gaaagtactt attgtagctg ggcaagccaa
     7621 aagtgttggg atatatgctt taatgattgc aaaagcattg ggcgttgcag aaattgatta
     7681 tgttgacaat agcaaagagc gattagaaat tgctcagaaa ctgcaagcca ataatatttt
     7741 tgaaagcttc catcaagtaa cagggaagta tgatttggta attgacacaa gttcaacaag
     7801 caaggggtta gaaaccactt tgaaatctgt cagacatttt ggaactatat cgagttcggg
     7861 tatttatata aaaaaaacac ctttgtcatt aattgaaatg tatgcaaaag gcgtaaattt
     7921 taaaattgga ctaaccaatt cacggacaga tggagaaatt gtattaaggc tgttagagaa
     7981 aatagaaatc ccttttgagt tagcaacaac gaaacttgaa agttgggaga acgcagaaag
     8041 cgcttttctt atagaaacaa caaaggtaat tgtaaaacgg tgtcttagcc cctaattctc
     8101 tgcacaaaat ctgaaaaaac agatttgaaa taattggaaa agttagcaaa tgtaaatcgt
     8161 ggccaaattt tattttacca gtttcttgaa cctttggaaa ttacgacttg ccgtctttaa
     8221 aaggatttga aaatactgtg gatttgaatt tctcaaatta ttaaatggac acacttcaac
     8281 aaactttctc tgcaaaaata attcagcaaa cttcagggta agcgtacggg aattatatgg
     8341 gaatgataat ttgatattta ggtttctata tctttcactt tcacattaga ctcctgtcat
     8401 caccgccact acaagggcta cagccgcgag tgttaaaata atgagcattg gcttccaaat
     8461 aaatctaaac caattatcat aggaaatgcc cgcaattgcg aggactgcca tcagggctcc
     8521 gttggttgga acaatcagat ccatcatcac tgctccgtat tggtaggcca gcacgcaaac
     8581 ttgcctggac aatccaatta aatctgataa aggcgaaagg atcggcatgg ttagtattgc
     8641 ctgccccgaa taactgggaa tcgggtagtg cagtaatagc tgggaaaaca tcatggtgat
     8701 agccgaaaga ccggcaggca aaaattgaag gggagtaaaa ataccataaa cgatggaatc
     8761 aataataatt cctttttgaa gaatcagtgg aatactattg gccacgccga taaggatggc
     8821 ggcaaaagtc atttcccgga atccttcaac ataagcgatg gcggttccat taacacccag
     8881 gtggccaatt aatccggcaa cgacgccaac tacaaaaaac agggcagaca tttcatagaa
     8941 accccattcc agtttcacca tcccataggc caggtagctg aaagcaagta ttaacattcc
     9001 aaatatcacc atacccctgc cactcatgga agtggaaccg gattcacttt tttctttctg
     9061 aattcgattc ctatttccgt agcggattat catcaaaacc catagcccta atgctgccaa
     9121 cataactacc aacctgaaac ctgaacctga cagtaacgga actccggaaa ttttctgagc
     9181 aatcagcacg gcaaaagggt tcatagggct gaaagcccca ccgatgatgg ccgctccaaa
     9241 acttacagaa acagcaacaa atgtgttata gcctaatttt ctgcagagta aaatgagtac
     9301 cggtgtcatg gcgataattt cttcctgaag acctgccaat gcacctcccg tagcaaaaat
     9361 gacagccaca acaaccaggg ctatttcttc cttccctgtt acctttccgg caaggaaagt
     9421 tataccctcc cgtaatgcac ctgttttttc aactacatag aaacaaccac ccaacaggaa
     9481 aacgagcaca attacatcag cgcggcccgc gataccttcg ggaatagaaa tcaggatttc
     9541 ctttacggta attcgctcag tagcaacggt ttggtaagaa tcctgaacaa cccgttcatt
     9601 tccttcagaa tccggttgcc gttcgtattg accttgcgga actatgaacg cgaggatgca
     9661 agagaaggca aaaaagccaa acaggatgac taaagcatgg ggaaatttct gttctgacat
     9721 aaaaaaaggt gcttcaaaga aagcacctaa tgtaacctta ttatcccctt cttgtcaatg
     9781 gaaatttatc atttataaag ttgatgtagc ttactgcttt ttacgcaggt agacgttccc
     9841 aaattttgat tccagcacaa ttttttttcc gcccccattc aaatgcgcca ctaatttctg
     9901 agccttgtag ggacgcaatc catcctggct ttccaattcc aggtcgaggt ttgtatacac
     9961 ttctccaaag gtggtatgaa tttcaaaatc atatcttgat gatgggtcga cgcttatgtc
    10021 aacgccgtca tgtttcgaag tggctacaag ctgctccggt aatcccagaa tttcaatcgt
    10081 tccaaacttg gaagtaatgt tcaggttgct gttccggggt actgttaccg ttaattcgat
    10141 gtcgatatcc aatccatttg aaatccaatt aattgcgctt ttatcatgtt gatccagaaa
    10201 ttcctttatc ttgggatcat tccagcttgc agccatgaat acgtatttct ctcctttcac
    10261 attaacagta atcctgtgtg gaatttcatc cttgtctttt attgtgctgt agatatctat
    10321 attatttccg gaggaattta ctaccaaatc aaaattttga tcaaataaac ctgaattaat
    10381 ggatactatt ccttccactt tgatttcatt aaggtcatga acctgaacct ttatgatttc
    10441 aggatatgta aattccatgg tgagtgtttt acctggctgg taaggtatgg ttctttcaaa
    10501 ccgcgattga gctaataagg agttggtaaa aacttcaaac agcagcagag ctggtattat
    10561 tcttttcatg ataataaaag ttaagaaagg aagaccaaag ccttcctttc aatccatact
    10621 tacttcttcc ttagataaat attatcatgc gtggagctaa gcatcagggc aactcctccg
    10681 ccattaagtt tcccccgaac ggtttgagaa cttatccttt tcatatccgc gctggtatcc
    10741 atctgaatat ccatgtcggt aaaaatttct ccccaggttg ttttgatagt gagctcagct
    10801 ttggaagatg ccggcactga aacatcgaca tgcccgtgca ctgaagcgat ggatgttggg
    10861 cttgcctggt ttagggttgt aaaatcggca tcaatatcgc catgaactgt atttattgtc
    10921 atgggacctg ttacatttaa caggagcaca tcattatgta atgatgatac atcaagttct
    10981 gattcgaggt tgctgatttt aagatccgaa ccatacggac tgttgtgcgc atatttcact
    11041 gaaacattct ttggtacttt tactatatac ctggattcag agtttctgga gatggaattg
    11101 acttctacta ctccattttt ttccacagct gataatccca gtccggtgtt atcttccaac
    11161 cccatcgaac taatagcttt gagccctgtt gcccgttcat ccttcgccct ggatcgatct
    11221 gttgttgtaa taatgatttc agaacctgtt gttccttcaa tctccaactt gtttatctcg
    11281 ttgaagttaa ttttcaatga tttgcccgaa gccggaattt tgtattcctg accaaatgac
    11341 ggcattgaaa atgcagcgaa tatcagtgcc actgcgatag tccaaatttg ctttttcatt
    11401 tccttaattt ttaatgtttt cattttaatt attttgaatt tttgacctta catcagtttg
    11461 aacaagccat attgtgcctg gtcctgaact gattgaatat tcttatcatc atctatcaaa
    11521 tcttgcagct tttctaccgc acggatttca ttgatattta caagagcatc aatgagagaa
    11581 atcaggacga ttggttctgt ttgttctccc aaagattcta ttagcgccaa ttttactttt
    11641 tctgatgaag ataacttggt cagcgcgttc aatgcagcca gtctgacatt tacattttca
    11701 tcttcattta aagtttcaat taaaacatcc atgatctggt cgtccggctg cttcatatct
    11761 tcaacataat tcacccccat aattcgttga cttggtgatt gattctgcaa tgccatcatt
    11821 accaactgcc gcgtagcttc catttcttcc cgtaaagaag ctaattcatc tttatccgta
    11881 ctgaattgtg ttcccagaaa atatcccaaa atcaaaattg ccaggccggc agccacgcgc
    11941 atcgtccaaa tcattggcat agaaattacc tttccacgtt ttgctgattt cgaggcggca
    12001 agcttcatct cctcctgcaa cattttgtca aattctgtat gcaatgttgc gtcaggttca
    12061 aactccctgc tctgattcat tacccggttc agctttttta actccccgaa ttcttttctt
    12121 gcatccgtgt tattttcaat atacctttcg acatattccc ttagctcgcc ggttaaattc
    12181 ccttcactat gttcaattaa taagtcgtgc attttctctt tatcaaattc tttcataact
    12241 attccatttg tttttcaatt tcgaaatagg tttgcctcaa tttctttata gcgcggtgaa
    12301 ccttaatttt aatattagac accgttgtat ttgttatcgc ggcaatttct tcatatttca
    12361 tttcctgaaa tctgcttaac accagaactt cccgctgatc atccggcaac ctggccaatg
    12421 aacgatgaag tattttttcc tgttcctgat gctcttcgct tttatgttca tcaaatgttt
    12481 gtatacccat tttttccact tcatcaaaat cagacatcca cttaccatca ctttttctga
    12541 aatgatcagc cagcacattt ctggctatct gataaatcca gggtctgaaa ctggcacctt
    12601 ttctgtaaga ttttctgtat ttcaacattc gtaaaaaaac attctgtgtt aaatcgtgac
    12661 ttagatcttt atcaaaagtc aatcggacaa aaaaagaata aagcttctta ttataactct
    12721 cgaataaggc cgaggctttc tgcaggtctc cctgcattac ctgttccatc aagaactcgt
    12781 cattggtttc agtcatcaaa gagttatttt tcttttgccc tcaggttggg tacccgaatg
    12841 aatagtaaag gttacagtta tttttgaaaa ttgcctgaaa ttggattaaa acctatagaa
    12901 tgaggggggt gaatttgata tcaacgcgcc ggttcatcat ccttccctgt ggaagcatgt
    12961 tatcgtataa tggagtgtgc tgaccacggt catcagttat gatttgctcc aacccgactt
    13021 cattgagcaa aagttcccgc accactgctt tgctgcgctg ggttgataac cagttgttat
    13081 attcaatccc gccaatatta tctgtatgag agataacagt gatggtgtag atggagagat
    13141 tttccagaga cttaacaaac tcctttaatt cctccatctg ttgttgatcg atataccaac
    13201 ttcccccacc gaagtagatg cttttgataa tttctttgtg ctgaacctct tcggtttctt
    13261 gggcgaatcc gaacgttgtt gtaatcaaca aaccggtaag gaacaaaatt ctcctcattt
    13321 caggattttt ttgcaatttg aattgcgact ttctccaatt taagaaaaag aatttgaaat
    13381 tgttcagtcg caaaaatcaa tccatcatta tttttacagc caaagttgga atagagtaca
    13441 tttgatctga tgtctgtttt aatcattgca ccgcaacggg acatgaagga atggaaaaaa
    13501 gctattcaaa atgttgatgc ttcccttcat atagaaattt atccggaact taataatccg
    13561 gaaagtgtca aggctgcggt tttatggaat caccctccgg gtattctgca taattttccc
    13621 aacctgaaat gggtaacctc gctgggagcc ggagttgatc atattctgaa agacaaaaaa
    13681 attcattccg atattagaat taccagaata gtcgatccgg gcatagccat ccaaatgtca
    13741 cggatgatcg taacgatgct cacatttttc gaaaaagatt tacttctgaa ttggaatccc
    13801 gggaaaacag gaggttggaa tactatttcc cctgtacctg aattgaaaat tggattgatg
    13861 ggcattggtg aaattgggaa aaaggtagcc agtgtgctaa ctgagttaaa atacgaagtt
    13921 gttgcctttg gaaatacaag ccgggtttac aagaatctta ggatctttgg tcaaaatgag
    13981 cttcgacaat ttttaaaatc agtagacgtc gcgatatgtt tgcttcccct gacatccaaa
    14041 accgagaatt ttataaacaa ggatttcttt tcgctcatga ataaaggaag ttatattatg
    14101 aacctcgcgc gcggaaaaat tgtggatgaa gatgctttac tttgggcgtt gaaaacgggg
    14161 attcttaaga aagcgtatct ggatgttttc aggcaagaac cgcttcccgc aacccaccca
    14221 ttttggagta atccggagat aattattacg ccccacatct caggaataac agatgttatg
    14281 gaaggcgccc gtcaaattgt aaacaattat catacatttg aagattcaga atttcttatt
    14341 aatgaaataa acagacttaa agaatattaa catgaaagat gaatttgacc ggcttgttaa
    14401 actgactggc gccgtatacg atggtgaacc gtggtatggt ccgtcagtaa aaagtatctt
    14461 atccgatctc accgaagatc atcgtgtatt aagaacagga gagtcacatt ccataaatga
    14521 attgattttc catatggttg cctggcgaaa tatgatcgct caaactctgg aaggaaacaa
    14581 tgagtatgtc gttagtgacg aagaaaattt caagccgcat gctaataagt attccctgaa
    14641 ggaagctacg gaagccctgg aaatgtcaca ggttttactt ctaaaagctc tttcgacttt
    14701 tgatgccgac aaactccagg aaaaagttcc aggaaagaaa tatagctatt atcagttgat
    14761 acaaggaatt attcaccacg atgtgtatca tgcaggacag atttccctgc tgaaaaagaa
    14821 aattcagagt aactaacaaa aataaatttg ctatcccatg aaaaaatcaa ttacactgtt
    14881 cgcttcgtta tttattagtc tgacaacttt ttcccaggtt ttgaaagccc caccacgagc
    14941 tgaaggcgat ggtccctggg atcaacttat aatccgcgga gtaatcctgg tggatggtac
    15001 aggagctcca ccatttggtc ctgttgatat tgtggttgaa aaaaatatta tcaaacagat
    15061 agccaccgta ggttctcccg gagtggaaat taatccgaac agaagaccgc aactgaagcc
    15121 cggggggaag gaaattgatg ctaccggaaa atacctgctg cctggttttg tagacatgca
    15181 tggacatatt ggtggttcag cccagggagc aaatgcagaa tatgtattca aactttggat
    15241 gggacatggt gttactacta tccgagatcc atccgccggc aatggtttgg attgggtttt
    15301 agaagaaaaa gacaagagta aaaaaaacca aattactgca ccacgaatta tggcctatac
    15361 ctcgtttgga tccggagcta aagagccggt tacaacacct cagcaagcca gggaatgggt
    15421 caggcaaaat gctgccaatg gcgctgatgg cattaaattt ttcggcgcgc caccagaaat
    15481 tatggctgca gccctggatg aaaataaaaa attgggttta agaagcgcct gtcaccacgc
    15541 tcaattgaat gttgcctggt ggaatgtggt taatagcgcc agggccggac ttacctccat
    15601 ggaacattgg tatggattgc ccgaagcact ctttaccgac cgcgctatcc agaattatcc
    15661 gttggactat aattaccaaa acgagcaaga tcgatttagt gaggctggcc ggctctggaa
    15721 acaagcagct ccaccctatt cagaacattg gaataatgtt atggatgaac tgatttccgt
    15781 ggactttacg ctcgatccca cattcaatat ttatgaagca agcagggatt tgcaccgggc
    15841 acgcagacag gagtggcatg aagaatttac catgcccaaa ctttgggagt tttatcagcc
    15901 gagcatgatt tcgcacggaa gttactggca ttactgggga accgaagaag aagttgactg
    15961 gaaacagaac tataaattgt ggatgacctt cgtaaatgaa tacaagaacc ggggcggccg
    16021 tgttaccacc ggttctgatt caggattcat ttttcagtta tatggatttg cttacatccg
    16081 tgaattggaa ttactgcgtg aggccggttt ccatccgctg gaagtgattc gtgccgcaac
    16141 gctgaaaggc gcggaagcac tgggattgga caaagaaatt ggtaccatcc aggtaggaaa
    16201 acttgctgac atgatcattg tggatgagaa tcccctgaag aatttgaaag ttttgtatgg
    16261 aacaggagca attaaattga atgaaaataa cgaagtaatc agagtaggtg gcgtacggta
    16321 taccgtaaaa gatggaatag tctacgatgc caaacaatta ctggaggacg ttaaaaaaat
    16381 ggtggctgaa gaaaaagcta agaccgggtt tgaaatttac cagccgggta tgaagaaaat
    16441 taatacaaat cgtgagaatt agaagctggg ttggatttgc ctttctgtgt attccctttc
    16501 gtgctgaggc acaaagtata gatgaatatt ttgaccttgc cagggctgag ttccgtgggg
    16561 agaacgcttt tgaatccgtc gcgtatattg agcagttctg gcggcttgcg gggaattccg
    16621 ggttcgacaa gagtatatat catctggaag aaaaacttaa gcaggctggc tttgaaaagg
    16681 aaggcgccgc gaatggaagg ttaacctacc ggattgaaaa gagaaaactt gaagaaccaa
    16741 gttgggaacc catcagtgcc tctctcactc ttgaaggatc ttcatcgccg ctcctggatt
    16801 ttgcaacaaa tcgtaacatg atctccatca attcacattc aactaaaaat gaaggaattg
    16861 aaggagaagt ggtacagctt gctgatggac ggattccggc gggtgtagta gtgaaaggta
    16921 aaattctcta cggtgaaata agtccatttc gagcctacac gttggctgct gaatccggcg
    16981 ccataggtat aattacttat ggcaaccctt cctatcttaa accggaaatc aatacccgtt
    17041 ctattcagtt tcgatccatt ccttacaacg ccggccaaaa gttatggtca atttgtcttt
    17101 cctttgaagc caatgaaaag ctaaaagctt cactgatgtc cgggaaaata aaggtgcggg
    17161 taaaaattga aactgcattt ccggtttctg atgaacttac cctggttgcg gaaatcaaag
    17221 ggtcggaatt acctgaagaa aggtttgtgc tgagtgccca cttgcaggaa cccggagcca
    17281 atgataatgc ttcaggggca ggtgcgctta tggaaatggc aagggtttcc gcgacctgga
    17341 taaatgaaaa gaaaatagat tcgcggagaa cgataacgtt tctgtggggc gatgaaattg
    17401 tgagcacacg caattttgta agcgaagact ccgcacgtag aaagaatatt aaatggggac
    17461 ttagcctgga tatggtgggt gaaaatactt ctttaacggg tggcaggttc cttattgaga
    17521 aaatgccgga cccatcggcg atttggaccc ggggaaatga tcagcatacg gaatggggcg
    17581 cgggtgatgt aaaggaggaa gatttcttcc ctcattattt taacgatttc gttcttgaag
    17641 tatttcgcag gcaggcggaa ttcgccaact ggaatgtcag ttacaatcca tttgaaggcg
    17701 gaagtgatca cgttcctttt ttacaggcag gaataccttc cgtcttgtta tggcatttta
    17761 ctgatcagtt ttatcatacc gatttagata gaatcgataa agtatcgaag caggaattgg
    17821 ccaacgtggg aaacggagcg ttggttgttt tatttactct ggttaacgcg gatgccaata
    17881 ccgcgggttt tctgattaac caaaccttta aagccggaaa agcaaggctt tccgcggaag
    17941 cagacctctc gaagaaagca attcaacttg gcaggagcaa acaggaggag ctactaataa
    18001 ttgacgcctg gatccggtgg tacctcaatg cgattcattc cgcgaaggac atagcttctt
    18061 ctgatgccgg gttcacccaa attaatcacc tggcaaacca gaaagcagca gaatttcaac
    18121 agtttgccag gagcttaatg tctcaattta atgattaggc aactaattag ttgctattat
    18181 ttgcaagatt aactatatta gcaactattt agttgccaat gcctaatata gaacactcct
    18241 ttaaagcctt agctgattct acacggcggg aaattgttta tatgcttgct gttacgcctt
    18301 ccggtctgtc aatgagggag attagcgacc agtttccggc atccaggcaa ggggttgcca
    18361 aacatcttgg cgttttagta agcgccggct taattcaaat tcaatcagca ggaagggaga
    18421 aaaaatgcct gctgaaccct ttgccgctcc gggaggtaat ggattggctc aatttcttca
    18481 agtcttattg ggaagacagg ttgaatgaat tgaaagctca cggtgaatcg gattaaccgt
    18541 ttggtgtggc cgggtaaaac ctgccccggg gcttacatcg acattccttt gtcgtttccc
    18601 tttttcttaa atattctttt aaaaagtgat tgttgcttgt taatggtttt tgcttcacct
    18661 ctttcaagga tctttcttaa ctcctgagca tccactacgt ctttcaagct tccatcatat
    18721 cgctttacat tgctctttat ttcaattaag tctttaagac taattattgg taaattaaag
    18781 ctttctaatt tctgtacttt agccctctca tagcactttt caaaagtcaa aggttcataa
    18841 aatattgaag tcatcaggtc aactttaaat ttgtcatctt ct
//
LOCUS       DASWHX010000117        13496 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_2335181, whole genome shotgun sequence.
ACCESSION   DASWHX010000117 DASWHX010000000
VERSION     DASWHX010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 13496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13496
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2335181"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..713)
                     /locus_tag="VGA21_08155"
     CDS             complement(<1..713)
                     /locus_tag="VGA21_08155"
                     /inference="COORDINATES: protein motif:HMM:NF025420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4249 domain-containing protein"
                     /protein_id="HEX9650518.1"
                     /translation="MRNRIYIFCLLVLMACIDPLDYSTEREEKLLVVEGFITTEPGPH
                     TVTISLSARYGTFADGIIQRVRSAKVNVRDSDGLITEMNEVQNGIYQTPASFHAFPGK
                     SYSLQIITQGGKRYFSLPEQAVSAIPIDTLFATYKRLPSSNPANFVSGAEIWTRFKDP
                     SEERNYYMWQANGTYIINTHPENYFDRNTFAPAPKDCCATCWIEETYNNGVSILSDFN
                     LDGNTVNWPVAFIEDDGGR"
     gene            complement(713..3103)
                     /locus_tag="VGA21_08160"
     CDS             complement(713..3103)
                     /locus_tag="VGA21_08160"
                     /inference="COORDINATES: protein motif:HMM:NF025092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX9650519.1"
                     /translation="MRKVYPFVFILYFFTQVSWAQKAIEIGKGSNRVGEVLIIGKVMD
                     YETGETLIGATVIVDELKKGEVTDINGMFGLYVPVGEYTLTISSVGFQELKQLIIVNG
                     TGEFKLRLLASITELEEVLIQSQEADVNVKSTDLGKNTLSMESIKALPPFAGEVDILK
                     SITLLPGVTSVGEASSGFNVRGGGSDQNLILLGGAVLYNPSHLFGFFSAFNSDLIRNV
                     IIYKGGIPAKYGGRASSILDLEYRKGNNKRWGVKGSLGLVSLKLAIEGPIVKDKLSVL
                     LGVRTSYSNWLLKSINDANIKNSSTQFYDLNGLVNYQLNNKNDLNYSFYRSYDDFKFA
                     SDTTNAWTNMAHVFDWKHAFAEKTIFHSIFSRSDYEFSINNKVGTNNFSINSKIIDQG
                     VNLGLEFGVGKKGIVLVGVQVKQIEIDPGNLLPINEQSAINALDLESEQAIEGGAYAQ
                     HDFEIGKLGVSYGLRYNRFYYLGSNTIYQYEENLPRNEENIINQTTFGKGDVIKDYDG
                     FEPRLSLRWSINTNTSIKLGYNRMFQYIHLISNTATISPTDIWKLTDQFVKPEIGSQI
                     SFGIFKNFSNNTIETSVEGYYKKLQNIVEYKDGADLIMNEHIETELLNGDGRTYGVEL
                     YVKKTFGRLEGWASYTYSKSLREVKGPYPEETINLGKEYPSNFDKPHDLTAVIDYSIN
                     DKVKFSSIFTYSTGRAVTTPSAKFGYYNQNVSFFNARNNGRAPDYHRLDVSLTFSFQS
                     RHRFLQGDWVLSVYNLYGRKNAFSVFFDDVDGAPPQAYKMSVLGIPFPTLSYNFEF"
     gene            complement(3105..4598)
                     /locus_tag="VGA21_08165"
     CDS             complement(3105..4598)
                     /locus_tag="VGA21_08165"
                     /inference="COORDINATES: protein motif:HMM:NF013955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HEX9650520.1"
                     /translation="MVLFYRFKYLVASLILSLTVAACEEVLDNTEVIVVSEEVVYISG
                     ETVRITGRILSLPSKGVDDRGYLIAEDENFANPAKVSLGPTDLPGRFITEYNQLSTGS
                     NYFAKSYVEVDREFFYGSILNFSTLEPFVQNFAPMFGVGGMILTITGGNFTSDTKVIV
                     GGVEAEILNIFFESKITLRMPPINTTSTVDIRVVVQGEELLFNQVFEYVVGLWKLEEH
                     FITQAQLFDPVYFVDGNSLVVGLGRDLNTGDNSNFWKADLNTWQWDELTFTGSFARLF
                     FFNEGFFGGGIFASGGFFGAPWVPNNEFWTYDSNSGSFVQQTDLTFNIAGGKTIKTAN
                     GFYLTGGTRPDNSPNPNIYFYDVGTQQWNTYTAAATSLPQRAIYFEYQDALYFIDQNN
                     IWRYDIISEDLQYITTYPGDGDEVGIAEVIRDKVYVGLFANSGEIKEFDLTSNSWKEK
                     TFFPGLTAESNSASWVYNDKIYVMRTTRTAFSNSNRMQIWTFEPDKF"
     gene            4876..5844
                     /locus_tag="VGA21_08170"
     CDS             4876..5844
                     /locus_tag="VGA21_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013453348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX9650521.1"
                     /translation="MSWFKSFGFEELVFISLFILFYGIYIFRIVAIAKKLRTTYTGIF
                     VKVAVRTLFFSLLIIALMGPSFGDAKREIKSVGKDIYIAVDLSQSMNATDIQPSRLER
                     LKFELKNIVSSFSSDRLGLIIFSSEAFIQCPLTYDQNALNLFIETLHTGLVPRTGTDF
                     GPPLRMALSKLDDDENPVSQPKSKIVILISDGEDFGDDTEQIASNVEDAGIKLFTLGI
                     GTERGSRIPSGRGYKKDDQGNEVVTRLESNSLRQLAVKTGGNYFEINETNNDVLRLIN
                     KVNQIEGELRESRQVDVSANKYYFFLLAAFLLLLVDAVTSFKTITI"
     gene            5841..6632
                     /locus_tag="VGA21_08175"
     CDS             5841..6632
                     /locus_tag="VGA21_08175"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9650522.1"
                     /translation="MKYLLIILVAVLSVGNEITKIARVNQLKREAKEAYQNGDYAIAI
                     EKYRFLVDSFKVADEEVVINLSNAFYESNDTAAAISYYQQLTRSSNSGLRSQAFQQLG
                     VVDAQRKQYEQALSSFKQSLKANPANDDSRYNYELLKKMVKEQEDQQKQDQNQQNKDQ
                     QNKDQNKQDQEKQENQEKQDSEKGDQENQEEQNQEEKKEQEGDQEKENEPQPESTKPE
                     KLKEMNISEEKARMILEAMKNNEIQYIQQNKRKAKEPKKSTKPDW"
     gene            6711..7889
                     /locus_tag="VGA21_08180"
     CDS             6711..7889
                     /locus_tag="VGA21_08180"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010610308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEX9650523.1"
                     /translation="MKEAYIISAIRTPIGSFGGSLSSLSAITLGSAAIRGAILKAGIE
                     ATQVNEVIFGNVISANLGQAPARQAAVGAGIAYNVPCTTINKVCSSGMKSVMYGAQSI
                     MLGYSDVVVTGGMESMSNVPYYVPKARFGYKYGNGEFIDGLAKDGLWEVYYNFPMGSC
                     ADNTAKEMKITREEQDEYAVNSYKRAASATDAGYFKNEIIPVEVPQRKGDPVSVTTDE
                     EYKNIYFDKIPSLKPAFNKDGTVTAANASTINDGAAALVLMSKEKAEELGVKPIAKIR
                     GFADAEQDPIWFTTSPSLAIPLAMKRAGVKKSDVNYYEINEAFSAVAIANKRQMELDY
                     DQINVFGGAVALGHPLGASGARIIATLNNILTVKNANIGVAGICNGGGGASAIVIEKV
                     "
     gene            7940..9460
                     /locus_tag="VGA21_08185"
     CDS             7940..9460
                     /locus_tag="VGA21_08185"
                     /inference="COORDINATES: protein motif:HMM:NF019284.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650524.1"
                     /translation="MNRALISAFLLLTFEGYTQKIVETYYNLGEKSVKEQYSVLDSDS
                     KIFHGPYKLFDPDGYIKEEGNYIQGAKEGHWKFYHSNGVVSAEGNYHQGKMDSTWAYY
                     SREGYLIHQGKFVNDLKDGEWRAYYPGGAIQGEGNFVAGNGTFTEFYPNGRKQRTTQY
                     KNGLKEGPVLVYGEAGELMQVSSFKNDVLSDSMLTYYPGGNIRQRSFFVDGNPEGVVI
                     EYYENGIISKEINYSKGEINGISKSFYPDGVVKVEAEYTNGILNGFYKTYYSNGQIET
                     ESQNVNGNKNGEFTTRNREGVLINKGAYRNGKLHGINLGFYSNGKPRHTFVYEDGFKE
                     GENKSFHENGMIKQTGNYANEEKTKQITTYHSNGKKESQMEERNGKPFGTWTTFYQNE
                     QLKSKDTYENGLLNGVRITYFENGRVQLEEPFNKGSKQGIEKSFFDNGNLKSATTYKS
                     GKLHGECREYYLDETLKEEGEYAGNRKVGKWKIYDETGNLSKLLIYENGKLVEEVK"
     gene            9523..10479
                     /locus_tag="VGA21_08190"
     CDS             9523..10479
                     /locus_tag="VGA21_08190"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020600626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEX9650525.1"
                     /translation="MSAIKETHFSFPGQTGFYRGKVRDVYYFDDKIVMVATDRISAFD
                     VILPEPVPLKGAILNTIAFKNLKATEDIVKNWCLDMPDPNVTIGVKAEPLPVEMVIRG
                     YLSGHAWREYKEGKRSICGVPIPGAMKENDKFPKPIITPSTKAIEGHDEDISKEEIIK
                     RGLVEKSDYEKLETITLQLFERGTEIADERGLILVDTKYEFGKFKGEIVLIDEIHTPD
                     SSRYFYKDGYAEKQEKSEPQRQLSKEFVREWLISEGFMGQKGQTVPEMTEEIVSTITA
                     RYQELFEKFTGGKMEQFKMDDIENRIKENIDKSFKKLNFNLN"
     gene            10509..10895
                     /locus_tag="VGA21_08195"
     CDS             10509..10895
                     /locus_tag="VGA21_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013453664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX9650526.1"
                     /translation="MKYSVDKQEKYSLIKLIEAKLDTTIAPGLKSEFITMHAEGIKNI
                     ILDISDVNYTDSSGLSALLVGNRTFRQNGGMFVIASPSDHAMKLIKISQLDGVLNLMA
                     TVEEAVDAVFMNEIENDLKGREGNSN"
     gene            10905..11816
                     /locus_tag="VGA21_08200"
     CDS             10905..11816
                     /locus_tag="VGA21_08200"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013453663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HEX9650527.1"
                     /translation="MTFHLTILGSNSAIPAHGRNHTSQILVINNEHFLIDCGEGTQLQ
                     IARYKCKLSRINHILISHLHGDHYLGLIGLISSMHLQGRKKTLNLWGPAGLREIVSVQ
                     LKFSQTILNFPIYFSELDEESGVIYQNNFMTITSFRLNHRIPCFGFLFKEKKKPRRLK
                     KEKLPAWLQAKEFRQLKNGKDLTDPDGKIIKNTTLTDPPRKSRSYAYCSDTRYMPELT
                     SVIANCDILYHEATFLHENMEWAESTFHSTTVEAATLAKNAKAGKLLIGHFSARYKDL
                     SPFLEEARPVFESTELAIEGRTYSVDE"
     gene            11809..12600
                     /locus_tag="VGA21_08205"
     CDS             11809..12600
                     /locus_tag="VGA21_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010610314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HEX9650528.1"
                     /translation="MSNNIDKRTRLFIILGSIFLTNALLAELIGVKIFSLEQTFGYNP
                     VQIHLFGEFVLDFNLTAGAIIWPVVFITTDIINEYFGREGVRRITFITVAMISFAFLV
                     IWVITKLPGAAFWLEINSTDLEGNPFNPAYAFNFIFRQGMGIIFGSLVAFLLGQFLDV
                     FIFQKLRKYTGSKMIWLRATGSTLVSQLLDSFVVLTIAFYFFGNWSLQQVIAVGIINY
                     MYKFVVAIALTPLLYVGHFFIDRFLGKENADKMAEEAASKSHRLF"
     gene            12600..>13496
                     /locus_tag="VGA21_08210"
     CDS             12600..>13496
                     /locus_tag="VGA21_08210"
                     /inference="COORDINATES: protein motif:HMM:NF028437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5106 domain-containing protein"
                     /protein_id="HEX9650529.1"
                     /translation="MKLLRIFGFVFISLTSMSQGYELQFHVRGITDTVAYLGYHFGNQ
                     KYVQDTSEIRDNKAVFMGPKLLLPGIYFFYSANTFFEFVVDDQKFAINTSAPDFIKTA
                     EVKGSEINKDFMDLQRYNLVKQNEAKELQNRITTSEGEVKENLESELKNLNESAVGFQ
                     NIIIDKHKDDFLGKFLTALQKPKVPDSIGLEGNDREFARFAHYKTHYFDNFNLADESL
                     LRSPVYHSVITEYLDKITMQHPDSLISSADVILSQAMKTEATFRYCLVTLSNKFESSN
                     IMGFDKVFVHIIEEYYLTRKASW"
BASE COUNT         4284 a         2637 c         2728 g         3847 t
ORIGIN      
        1 aatcttccgc catcatcctc aataaacgcg acaggccagt tcaccgtatt tccatcaagg
       61 ttaaaatcag acaaaatgct taccccattg ttataggttt cctctatcca gcatgtcgcg
      121 cagcaatctt taggagcagg cgcgaaagta ttgcggtcaa aataattttc aggatgagta
      181 ttgataatat aagtaccatt agcctgccac atgtagtaat ttctctcctc ggaaggatct
      241 ttgaacctgg tccagatttc agctccggaa acaaagttgg ctggattgct tgaaggcaac
      301 cttttgtaag ttgcaaaaag tgtatctatg ggaatggcag aaacggcctg ttcaggaagc
      361 gaaaaatatc gttttccgcc ctgagttatt atttgtaaag aatagctctt acccgggaac
      421 gcatggaaac tagccggcgt ctgataaata ccattttgaa cttcattcat ctcagttatt
      481 aaaccatcgc tgtcgcgtac atttaccttt gcacttctga cccgttggat aataccatcc
      541 gcgaaagttc cataccgggc tgatagtgag attgtcaccg tatgaggtcc aggttcggtg
      601 gtaataaaac cttccaccac caataatttc tcttcccgtt cggttgaata atccaacggg
      661 tctatacatg ccatcagtac aagcaaacag aagatataaa ttctatttct catcagaatt
      721 caaagttata actgagggtt ggaaatggaa tgccgagcac cgacatctta tatgcttgag
      781 gcggggcccc atctacatca tcaaaaaata cagagaatgc atttttcctt ccatatagat
      841 tatatacgga caacacccaa tccccctgca gaaatctatg ccggctttgg aagctaaatg
      901 taagtgaaac atccaatcgg tgataatcag gagcccgtcc attatttctg gcattaaaaa
      961 aggaaacatt ctgattgtaa taaccaaatt ttgccgaagg tgttgtcacc gctctgccgg
     1021 tgctgtaagt aaagatggag gaaaacttca ctttatcgtt gattgaataa tcaataacgg
     1081 cggtcaaatc gtggggttta tcaaagttcg aagggtattc cttaccaaga tttattgttt
     1141 cctctggata ggggcctttc acctctctca aacttttgga atacgtatag cttgcccagc
     1201 cttctaatcg accaaaagtt ttcttcacat ataattccac gccataggtc ctgccatcac
     1261 cattcagcaa ttcagtttcg atatgttcat tcatgattag gtcagcccca tctttatatt
     1321 caacaatatt ctgcagcttt ttataatatc cttcaactga tgtttcaata gtattattac
     1381 tgaagttttt gaagattcca aaggagattt gtgaaccgat ctccggcttt acaaactgat
     1441 cggttaattt ccaaatgtct gtcggcgaaa tggtggctgt atttgaaata agatgtatgt
     1501 attgaaacat cctgttatat ccaagcttga tggaggtatt agtatttatg ctccatctta
     1561 acgaaagcct gggctcaaaa ccatcataat ccttaataac atcacccttg ccaaaggtgg
     1621 tttggttgat aatattttct tcgtttcgtg gaagattctc ttcgtattga taaatagtat
     1681 ttgaacccag gtaataaaat cgattgtacc gcaacccgta agaaaccccc agcttaccta
     1741 tttcaaaatc atgttgagca taagccccac cttctatcgc ttgctcactt tcaaggtcca
     1801 gtgcatttat tgccgactgt tcattaattg gcagcaagtt accgggatca atttcaattt
     1861 gttttacctg aacaccaact aaaacaatac cctttttgcc aacaccaaat tcaagcccta
     1921 gatttacacc ctggtctatt attttggagt ttatagaaaa attattagta cccactttat
     1981 tgtttatgga aaactcgtag tcactccgcg aaaatatgct gtgaaagatc gtcttttccg
     2041 caaatgcatg tttccagtcg aaaacatgtg ccatgttggt ccaggcattt gttgtatcgc
     2101 tagcgaattt aaaatcatca taacttctgt agaaactata attaaggtca tttttattgt
     2161 tgagttggta gtttaccagc ccattcagat cgtaaaactg agtggaacta ttcttaatgt
     2221 tggcatcgtt aattgacttc aaaagccagt ttgaataaga agttcttact ccaagaagca
     2281 ccgataattt atcttttacg attggccctt caatggccag ctttaaagaa accaatccca
     2341 gagatccttt cactccccat ctcttattat tgccttttct atattccaga tccaaaatcg
     2401 aagaagctct tcccccatat tttgcaggaa tgccgccttt atatatgatg acatttcgga
     2461 tcaagtcgga gttgaaggcg gaaaaaaaac caaacaaatg cgaggggtta tataacaccg
     2521 ccccgccaag caggattagg ttctggtcac ttccaccacc ccttacattg aacccggaag
     2581 acgcctcacc cacggaagta acacccggca atagggttat tgatttaaga atatcaactt
     2641 caccagcaaa tggtggtaat gcttttattg attccatgga gagcgtattc ttacctaaat
     2701 cagtactttt tacatttacg tccgcctcct gactttgtat caatacttct tctaattctg
     2761 taatggaggc cagcaatcgt aatttaaatt ctcctgttcc attcacaatt atgagctgct
     2821 tgagttcctg gaaacctact gaacttatcg ttagtgtata ttctccaacc ggcacgtaca
     2881 atccaaacat cccgttgata tcagttactt cccccttttt caattcatca acaatcacag
     2941 tagcgccaat aagcgtttct cctgtttcat aatccattac ttttcctata atgagtactt
     3001 cgcctactct gtttgacccc ttgcctatct caatcgcttt ttgtgcccat gaaacctgag
     3061 tgaaaaaata aagtataaat acgaacgggt aaacttttct catcctagaa tttgtcaggt
     3121 tcaaatgtcc aaatttgcat ccggttgctg tttgaaaaag cagtccttgt ggtccgcatt
     3181 acatagatct tatcattata aacccaggat gctgaattac tttcagcggt aagtccgggg
     3241 aaaaaagttt tctctttcca gctgttcgac gttaaatcaa attccttaat ttctcctgaa
     3301 ttcgcgaata agcctacata cactttgtcc cttatcactt ccgcaattcc cacttcatct
     3361 ccatctccgg gataagttgt tatgtactgc agatcttcac taataatatc atatctccag
     3421 atattattct ggtcaataaa gtaaagcgca tcctgatact cgaaataaat ggcccgctga
     3481 ggaaggcttg tagctgctgc tgtataagtg ttccattgct gagttccaac gtcataaaaa
     3541 taaatattag gattaggtga attatcaggt ctggttcctc cagtcaaata gaacccgttg
     3601 gcagtcttaa tagtcttgcc acctgcaatg ttaaaagtta gatcggtttg ctgaacaaag
     3661 cttcccgaat ttgaatcata agtccagaat tcattatttg gaacccaggg cgctccaaaa
     3721 aatcctccgg atgcaaaaat accacctcca aaaaatcctt cattaaaaaa aaacagtcgg
     3781 gcaaaggaac ctgtaaatgt caattcatcc cattgccagg tgtttaagtc tgccttccag
     3841 aaattactat tatctccagt attcaggtct cttccaagcc ccactacaag ggaattccca
     3901 tccacaaaat aaacaggatc aaataactgt gcctgggtga taaaatgctc ttcaagtttc
     3961 cataatccaa ccacatactc gaatacctga ttaaacagga gttcttctcc ctgaacaact
     4021 acccttatat caaccgtact ggtggtattg attggaggca tccttagtgt tatttttgat
     4081 tcaaaaaaaa tatttaatat ctcagcttcc actccgccaa caatgacttt cgtgtcggaa
     4141 gtaaaattac cccccgttat tgttaaaatc attcctccaa caccaaacat gggcgcaaaa
     4201 ttctgaacaa agggctcaag cgtactgaaa tttaagattg aaccatagaa gaattcacga
     4261 tccacctcaa cataggattt cgcaaaataa ttggagccgg tagacaattg attatattct
     4321 gttatgaacc ggcctggcaa gtcggtagga cccagggaaa cctttgcagg gttggcaaaa
     4381 ttttcatctt cagctatcaa gtaacccctg tcatcgacac ctttggaggg caaacttaat
     4441 atccttccgg ttattcttac tgtttcaccg cttatatata caacttcctc tgagacaaca
     4501 attacctctg tattatcaag cacttcctcg catgcagcaa ctgtcaatga caaaattaag
     4561 ctagctacca ggtatttaaa gcggtaaaat aaaaccattc ggttaattaa aaacccttga
     4621 atgtacttcc cacatcttga tttatcaata ggtttaggtt ggtaggctga tttatttgtt
     4681 tagtcaattc ttttagcaaa aattagaccg tttaaagcat ccttttcaat acattattca
     4741 tcattcagct aattataaca ggagattatc agaaatttca ctccttccaa actgggtgcc
     4801 gttgggttaa aaattattaa agaagaaaac ggcagttgtt caatctagat tgaagccata
     4861 cctttgtagg gtattatgag ctggtttaaa tcttttgggt ttgaggaatt agttttcatc
     4921 tctctgttca tattgttcta tggcatttac attttccgaa ttgttgccat tgctaaaaag
     4981 ttgagaacta cttacaccgg aatatttgtg aaagttgcgg tgagaactct ttttttcagc
     5041 ctccttatca ttgccttgat gggtccatct tttggagatg ctaaaagaga aataaagtca
     5101 gttggcaagg atatttacat tgcagtggat cttagtcagt ccatgaatgc aacggatatt
     5161 cagcccagca ggcttgagcg attaaagttc gaattgaaaa acattgttag ttctttttca
     5221 agcgacaggc tcggtttgat catcttcagc tctgaagcat ttattcaatg tccgcttacc
     5281 tacgatcaaa atgcgctcaa cctttttatt gaaacattgc atacggggtt ggtccccaga
     5341 acgggaactg attttggacc accgttgaga atggcattgt ctaaattgga tgatgatgaa
     5401 aatccagttt ctcaacctaa atcaaaaatc gttattttaa tcagtgatgg agaagacttt
     5461 ggcgatgata ctgagcaaat tgcaagtaat gttgaagatg caggaataaa gctttttacc
     5521 cttggaatag gtacggagcg tggaagccga attccttcag gcagaggata taagaaagat
     5581 gatcaaggca atgaagtagt tacgaggctc gaatcaaatt cattaagaca attggcagta
     5641 aaaaccggtg gcaattattt tgaaataaat gaaaccaata acgatgtatt acggttaata
     5701 aataaggtga accaaattga aggagaattg cgggaatcaa ggcaggtgga tgtttcagct
     5761 aataagtatt atttctttct tctggcggca tttttgctac ttctggtaga tgccgtaact
     5821 tcgtttaaga caatcacaat atgaaatacc tgcttatcat actggtcgct gttttatctg
     5881 ttggaaacga aatcacaaag atagcccggg tcaaccaatt gaaacgtgaa gcaaaagaag
     5941 cctaccaaaa tggagattat gccatcgcca ttgagaagta caggtttctc gttgatagct
     6001 tcaaagttgc agatgaggaa gtggttatta acctttccaa tgcattttat gaatctaatg
     6061 atacggcagc tgcaatttcc tactatcagc aacttacccg atcctcaaat tccgggcttc
     6121 gctcccaggc atttcaacaa ttgggagttg ttgatgctca gcgtaagcaa tatgaacagg
     6181 cattgagttc ctttaagcaa tctctaaaag ccaatcctgc caatgatgat tccagatata
     6241 attatgagtt gttgaagaaa atggttaagg agcaggagga tcagcaaaaa caggatcaga
     6301 accagcaaaa caaggatcag cagaacaaag atcaaaacaa acaggatcag gaaaagcagg
     6361 agaaccagga aaagcaggat tctgaaaagg gagatcagga aaaccaggaa gagcaaaatc
     6421 aggaagaaaa aaaggaacag gaaggtgatc aggaaaaaga gaatgagcca caaccagaat
     6481 ctacaaaacc cgaaaagtta aaagagatga atatttctga agagaaagcc cggatgatcc
     6541 ttgaggctat gaaaaacaat gaaatccagt atattcagca aaataagaga aaggcaaaag
     6601 agccgaaaaa aagcaccaaa cctgactggt aagcatttcc gtcataagcc ggaatttcac
     6661 ttgctatgcc ttatttttgt ggcattatct cgatattaat ccagtaataa atgaaagaag
     6721 catatattat ttcagcaata cgaacaccca ttggcagttt tggcggatct ttatcttccc
     6781 tgtcagctat cacgctcgga tcggctgcaa ttagaggagc aatactgaaa gccggtattg
     6841 aagccactca ggttaacgaa gtgatttttg gaaatgtaat ttcagccaac ctgggtcagg
     6901 cacctgccag gcaagcggca gtgggagcag gaattgctta caatgtacct tgtactacta
     6961 taaataaggt gtgctcctcc ggaatgaaat ctgtaatgta cggagctcaa tccataatgc
     7021 ttggttacag cgatgttgtc gtgaccggcg gcatggaaag tatgtcaaat gtaccctact
     7081 atgttcccaa agcgcgattc gggtataaat acggcaatgg agaatttatt gacggcctgg
     7141 ccaaagatgg cctctgggaa gtatactata actttcctat gggcagttgc gcagacaata
     7201 ctgccaagga aatgaaaatt accagggagg aacaggatga atatgcagta aactcctaca
     7261 aaagagcggc gtcagctacc gatgccggat atttcaaaaa tgaaattatt cccgtggaag
     7321 ttccacagcg caaaggggat ccggtttcgg tgacaacaga tgaagaatat aaaaatatat
     7381 attttgataa aataccttct ttgaaaccgg catttaataa agatggcacc gtgacagcag
     7441 ccaacgcctc caccataaat gacggggccg ccgccctggt gctgatgagt aaagagaaag
     7501 cagaagagct cggggtaaaa cccattgcaa aaattcgagg atttgcagat gcggaacaag
     7561 accctatctg gttcaccact tcaccttccc tggctattcc attagctatg aaaagagccg
     7621 gtgtaaaaaa atctgatgtt aattattatg aaatcaatga agccttttcc gcagtcgcta
     7681 ttgccaataa aagacagatg gaactggatt atgatcagat aaatgttttt ggaggtgcgg
     7741 tggcgcttgg tcatcctctt ggcgcatcgg gagcacgaat catcgccact ttaaataata
     7801 ttctaacagt taagaatgcg aatataggag ttgccgggat ttgtaatggc ggcggcggtg
     7861 catctgccat tgtgatagaa aaagtataaa tttacatatt acgatttgtg ttactgtcgt
     7921 taacttacca tacctgatta tgaacagagc actaatttca gctttcctcc tgctaacttt
     7981 cgagggttat acccaaaaga ttgttgaaac ctattacaac ctgggtgaaa agtctgttaa
     8041 agaacagtac tccgttctgg atagcgattc aaaaattttc catggccctt ataagctttt
     8101 cgacccggac ggatacataa aggaagaagg aaattacatt cagggagcaa aagaaggcca
     8161 ctggaaattc tatcattcga atggtgtagt cagtgctgag ggcaattacc atcaaggtaa
     8221 aatggacagt acctgggcct attatagcag ggaaggttac ctgattcacc agggaaagtt
     8281 tgttaatgat ttgaaggatg gagaatggcg cgcctattat cctggcggag ctatccaggg
     8341 agaaggtaat ttcgttgcag gcaacggcac atttacggaa ttctacccaa acggcagaaa
     8401 gcagcgaacc actcaatata aaaatggatt gaaagaaggg ccggtacttg tttatggcga
     8461 agccggcgaa ttaatgcagg tgtcatcttt caagaatgat gtccttagtg atagtatgct
     8521 tacctattat ccgggtggaa atatcagaca aagaagtttt tttgttgatg gtaatccaga
     8581 gggtgtagtg attgaatatt atgaaaatgg tattatcagc aaagaaatta attattccaa
     8641 aggtgaaata aacggaatca gtaaatcatt ctacccggat ggtgtggtaa aagttgaagc
     8701 tgagtatacc aatggtatct taaatggatt ttacaaaacc tattattcaa acggtcagat
     8761 tgaaaccgaa tctcaaaatg taaatggaaa taagaacggg gaatttacta cccgcaaccg
     8821 ggaaggtgtt ctgataaaca aaggggcata ccgaaacgga aagttacatg gaataaatct
     8881 tggcttttat tccaacggaa aaccccgcca tacttttgtt tatgaagatg gctttaagga
     8941 aggggagaat aaatcctttc atgaaaacgg aatgattaag caaaccggga attatgcaaa
     9001 cgaagaaaag accaaacaga taacgactta ccattcaaat gggaaaaaag aatctcaaat
     9061 ggaagagcgg aacggtaaac cctttggaac ctggacaact ttttaccaga atgagcaact
     9121 taaatcaaag gacacttatg agaatggatt actcaatggg gtccgtatca cttatttcga
     9181 gaatggcaga gttcagcttg aagaaccatt taataaaggg agcaaacaag ggatcgagaa
     9241 gtcatttttt gacaatggaa atttaaaatc ggcaacgaca tataaatcag ggaaactgca
     9301 tggtgagtgc agggaatatt atttggatga gacactaaag gaagaaggcg aatatgcggg
     9361 taatcgaaaa gtcgggaaat ggaaaatcta tgatgaaacc gggaatcttt ccaaattgct
     9421 catttatgaa aatggaaagc tggtggaaga agttaaataa gaattaataa accgctgatg
     9481 gcggtttttt tattgctacc ctcctgctaa atttgcccgc tgatgagcgc aattaaagaa
     9541 acgcatttta gttttcccgg gcaaacagga ttttaccggg gcaaggttag ggatgtttat
     9601 tattttgatg ataaaattgt aatggtggca actgatcgaa tctcagcgtt cgatgtaatt
     9661 cttcctgaac ctgtcccact gaaaggtgcg attttaaata caatcgcgtt taaaaatctg
     9721 aaagccactg aagatatcgt taagaattgg tgtctggata tgcccgatcc caatgttacc
     9781 attggagtta aagcggaacc tttgcctgtt gaaatggtta taagaggtta tctgagcggg
     9841 catgcctgga gagaatataa ggaaggcaag cgatccattt gtggagtgcc aattcccggt
     9901 gcgatgaagg aaaatgacaa atttcccaag cctataatca caccctctac aaaagcaatt
     9961 gaaggtcatg atgaagatat ttctaaagaa gaaattatta agcgcggctt ggtagaaaaa
    10021 tcagattatg agaagctcga aaccataacc ctccaactat ttgaaagagg aaccgaaata
    10081 gcagatgaaa gaggattaat actggttgat acaaagtatg aattcggtaa attcaaggga
    10141 gaaattgtcc tgatagatga aattcatacc cccgattctt ccagatattt ttataaggac
    10201 ggttacgcgg aaaagcagga aaaatccgaa cctcaaagac agttaagtaa agaatttgtc
    10261 cgggaatggt taatatccga agggtttatg ggtcaaaagg gtcaaaccgt accagaaatg
    10321 accgaagaaa ttgtcagcac aataacggca aggtatcagg aattgtttga aaaatttacc
    10381 ggcgggaaaa tggagcaatt caaaatggat gacatcgaaa ataggattaa agaaaacata
    10441 gataaatctt ttaaaaagct taattttaat ctcaattagc agcacattat tgtagcacat
    10501 taaaaattat gaagtattca gttgacaagc aggagaaata cagtttgatt aagcttattg
    10561 aggccaagct agatacaact atcgccccag gtctcaaatc agagtttatc actatgcacg
    10621 cggaaggcat aaagaacata atccttgata tttctgatgt caattataca gactcttcag
    10681 gattaagtgc tttgctggta ggcaacagga ctttcagaca aaatggtggg atgtttgtga
    10741 ttgcatcccc atcggaccat gccatgaaat tgataaagat ttcccagctt gatggagtcc
    10801 tgaatcttat ggcaactgta gaagaggcgg ttgatgccgt ttttatgaat gaaattgaaa
    10861 acgacctaaa aggaagggaa ggaaattcta attgatcccg ggttttgacg tttcatctga
    10921 ccattctggg atccaattct gcaattccgg cccacggcag aaatcataca tctcaaattc
    10981 ttgtcattaa taatgaacat tttctgattg actgtggaga aggaacccaa cttcaaatcg
    11041 caagatataa atgcaagctt agccgcatta atcacatcct gattagtcat cttcatggag
    11101 accattacct cggtttaata ggactgatct cttccatgca tttgcaggga aggaaaaaaa
    11161 cactaaacct ttgggggccg gctggcctca gggaaattgt aagtgtgcag ttaaagtttt
    11221 ctcaaactat cttaaacttc cccatttact tcagtgagct ggatgaggaa tccggagtga
    11281 tttatcagaa taatttcatg acaattactt ccttcagact gaatcacaga attccctgtt
    11341 ttggatttct tttcaaggaa aaaaagaaac ccagaagact gaaaaaagaa aaattgcctg
    11401 catggctgca agccaaagag ttccggcaac ttaagaatgg caaagacctt actgacccgg
    11461 atggaaaaat aatcaagaat accaccttaa cagatccgcc acggaaatcc agatcctacg
    11521 cgtactgttc agacacgcga tacatgccag agctgacatc tgtaatagct aattgcgata
    11581 ttctttacca cgaagctaca tttcttcacg aaaacatgga gtgggcggaa tccacttttc
    11641 attctacaac cgtagaagct gccaccctgg caaaaaatgc caaggcaggt aaacttctaa
    11701 tcggtcactt ttccgcaaga tataaagatc tgtccccatt tctggaagaa gcgaggcctg
    11761 tattcgaatc aacagaattg gcaattgaag gaagaactta ttcagtcgat gagtaataat
    11821 attgataaaa ggacccggtt gtttatcatc ctcggcagca tttttctaac gaatgccctg
    11881 ctggctgaat tgattggcgt aaaaatattt tccttagagc aaacctttgg ttataatccg
    11941 gttcaaatac atttatttgg cgaattcgtt ttggatttta atctgacagc cggcgcaata
    12001 atctggcccg ttgtttttat taccacagat ataatcaatg aatatttcgg cagggaagga
    12061 gtgcgtagaa ttacctttat aaccgtagcc atgatcagct ttgcatttct ggtaatttgg
    12121 gtaattacta aattgccggg tgcagccttc tggctggaaa taaattctac ggatttggaa
    12181 ggtaacccgt ttaatccagc atatgcattt aatttcattt tcaggcaggg catgggaata
    12241 atttttggct cccttgttgc gtttctatta ggccagttcc tggatgtttt catctttcag
    12301 aaacttagaa aatacacagg gagcaaaatg atttggttaa gggccaccgg atctaccctg
    12361 gtatctcaat tacttgacag ttttgttgtc ttaactatag cgttttattt ctttggaaac
    12421 tggtcgctcc agcaggtaat tgcggtggga atcataaatt atatgtataa gtttgtagta
    12481 gctattgccc tgacgccatt attatacgtt gggcattttt ttattgacag attcctgggt
    12541 aaagaaaacg cggataaaat ggcagaggaa gcagccagta aaagtcatag attattttaa
    12601 tgaaactact taggattttt gggtttgtct ttatttccct gacttcgatg tcacagggtt
    12661 atgaattgca atttcatgta aggggaatta ccgatacggt agcatatctt ggataccatt
    12721 ttggaaatca aaagtacgtc caggatactt ccgaaattcg cgataataaa gcggtgttta
    12781 tgggtccaaa acttttgctg ccaggcattt atttctttta ttcagctaat actttttttg
    12841 aatttgtagt tgatgatcag aaatttgcca taaacacgtc agctccggat ttcataaaaa
    12901 cggccgaggt aaaggggtcc gaaataaaca aggattttat ggatcttcag cggtacaacc
    12961 tggtaaagca aaatgaagca aaggaattgc aaaatcgtat aactaccagt gaaggtgaag
    13021 ttaaagaaaa cttagaatcg gagttaaaga atttgaatga aagcgctgtc gggtttcaga
    13081 atattataat tgataagcat aaagacgatt tcctgggcaa attccttaca gcattacaaa
    13141 aacccaaagt gcccgattca attggtcttg aaggtaacga tcgcgaattt gccagattcg
    13201 ctcattataa aacacattac ttcgataatt ttaatcttgc agatgaaagt cttcttcgct
    13261 ctcctgttta tcattccgtg attactgaat atttggataa gataacaatg cagcatccgg
    13321 atagtcttat ctcatccgcc gatgttattc tgagtcaggc aatgaaaact gaagccacgt
    13381 ttcgatattg cctcgtaacc ctttccaaca aatttgaaag ttcaaatatc atgggttttg
    13441 ataaggtttt tgttcacatt attgaagaat attatctcac ccgaaaagct tcctgg
//
LOCUS       DASWHX010000118        26515 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_1205808, whole genome shotgun sequence.
ACCESSION   DASWHX010000118 DASWHX010000000
VERSION     DASWHX010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 26515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26515
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1205808"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(351..2369)
                     /gene="rpsA"
                     /locus_tag="VGA21_08215"
     CDS             complement(351..2369)
                     /gene="rpsA"
                     /locus_tag="VGA21_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009581495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEX9650530.1"
                     /translation="MTESEENKEIKQEETNENSAGSETTNSSPEQPAEAASAVEELRK
                     TSSSKSKKELQKEAPGEFDWDALDQKGFGEGYSDKEREDLEKLYSGTFNQIPEKEVVT
                     GTVVDINDRDVVVNIGFKSDGLVSASEFRDTPDLKVGDKVEVYIEEQEDATGQLNLSR
                     RKAKIVGAWDKIQKAFDEDSVIEGFVKRRTKGGLIVDIYGIEAFLPGSQIDVKPIRDF
                     DVFVDKKMEVKVVKINYSNDNVVVSHKVLIEKDLEAQKAEILNNLEKGQVLEGVIKNM
                     TNFGVFIDLGGVDGLLHITDISWGRINHPQEVLNLDDTIKIVVLDFDEDKKRISLGMK
                     QLTPHPWDSLDKSVEIGSKVKGKIVNVADYGAFLELMPGVEGLIHVSEMSWSQHLRNP
                     QDFMKVGDAMEAVVITIDRDERKMSLGIKQLTEDPWTKQDLLTKYAVGTTHKGIVRNL
                     TNFGLFIELEEGIDGLVHVSDLSWTKKIKHPSEFVKVGEELEVKVLELDVENRRLALG
                     HKQLEENPWEAFEDVFATGSVHKCTVIEKVDKGVVLELPYGIEGFSANKNAAKEDGSR
                     PEVGETLEFKVLDFSKDEKRILLSHVATYKEEVKAPAKKKPIKKAAAKVSKEVEKSTL
                     GDIEALSELKTKMSETPKEGKSKKSKKAETDEESNNEQVVSEESKKEE"
     gene            complement(2728..4065)
                     /locus_tag="VGA21_08220"
     CDS             complement(2728..4065)
                     /locus_tag="VGA21_08220"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX9650531.1"
                     /translation="MKKFILLSLFIGCVVIAYAQVEQLSYKQALTYALKNNVTLQQTV
                     NQLMVNNALRTESILRYAPTISATGQAWISSGNTFLQQTGDVINTQSSNLFGQVQANL
                     LLFNGFSNFNTMRQARLAREAQETLIRRTEQDIINTITNNYLQVLLDQELVKIAGENQ
                     DNQQAIYDQIKGFVDVGQRAIVELYTQDAELKRLQSEVLRAQRNLRNDKAVLALNMGV
                     DPLKEFELADPTLDVNTVNLAEFNLDELVKIAMDNRADLESLNQSAMAAKRTIAISRT
                     QSLPRVSAFGGLSSRYANIQDRDDRPIGDQFLTDNVNKQVGIQINIPIFTGWNNRTPV
                     VRSRVAYENAELDVLFRELQIKSEVYNIYRSFQDFLNAYQASLAQREAAELAVQVQRE
                     SYELGIASQVELNQAMQLFVQAETTLASNKYRLIFQRILIDYATGTLSLESMP"
     gene            complement(4085..4786)
                     /locus_tag="VGA21_08225"
     CDS             complement(4085..4786)
                     /locus_tag="VGA21_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009581498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9650532.1"
                     /translation="MIELKDIDKYVDSRFQRTFILKGIDCQINEGEFVTIMGPSGAGK
                     STLLNIIGMLDEASAGEYHFLGEQVHKLKERKKSELHKHFIGFIFQAYHLIDELTVYE
                     NIETPLLYKGVSSSKRKSMVAEILDRFQMVAKKDLFPEQLSGGQQQLVGVARAIIGQP
                     KLLLADEPTGNLHSEQGEEIMEIFKKLNEEGMTIIQVTHSEKNATFSKRIIRLVDGQV
                     EADISSEELKASLNL"
     gene            5057..5557
                     /locus_tag="VGA21_08230"
     CDS             5057..5557
                     /locus_tag="VGA21_08230"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEX9650533.1"
                     /translation="MPPSLDKFVIIVGPHTSYLDFILGVAVRSVMQMRTHYLGKKELF
                     QPPFGFIFKMLGGYPVDRSSSKNMVDEVVKVFDAHEKFSIALAPEGTRKKVEKLRTGF
                     YHIALKAKVPLAMAALDFGNKEVIFSDPFELTGNKQEDFERIFAFFRNVKGKYPESGM
                     HNLKIE"
     gene            5560..7194
                     /locus_tag="VGA21_08235"
     CDS             5560..7194
                     /locus_tag="VGA21_08235"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX9650534.1"
                     /translation="MKTDPNLLDLFIHWELKTPDKPFLNEPVSGEYKTITWGEAGNQV
                     RKMANYLVSLSLPPKSHIAILGKNSAHWIMADLAIMMSGHVSIPLYPNLTSEQLNLIL
                     EHSESNLLFIGKLDQFEELKKGIPENMPCISFPFYPHPGYPTWDDLIADQEGGEFSPR
                     NNDDLYCILYTSGTTGTPKGVMHTFGNFAFAIKGIKEVVNLKNETFFSYLPLCHVAEK
                     MVIESASLLSGGAVFFAESIDTFAKDLAYSKPTLFLAVPRIWTKFQESILAKLPQSRL
                     NLLLSIPIINTILKKSIKKKLGLTRARIIFSGAAPISTSLLEWFKKLGIHIQEAYGMT
                     ENLSYSHFNRPGAIRIGSVGQALPRCEVKISEQSEVLVKSEATMRGYFKDQEQTNEMI
                     TSDGFLKTGDEGRIDEEGFLYITGRVKDIFKTSKGKYIAPAPIEKMIMSYELVSQTCV
                     VGSGLPQPIALITIPGKGTDIQENVIDHFKTILEESNEKLAAHEQLRKIVLFRQEWTV
                     RDILTPSLKIKRRSVEAIYGARFESWYEDAEDVIFG"
     gene            complement(7238..9634)
                     /locus_tag="VGA21_08240"
     CDS             complement(7238..9634)
                     /locus_tag="VGA21_08240"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9650535.1"
                     /translation="MVKNYLKIAFRNIANQKLYSFINIMGLTIGIAGSIFILFYVVDE
                     LSYDKFHTDAKRIYRVGLQGKIAGQEINVIYTCPPLANAMANEFPEVESAVRLGQNLE
                     ILFRYEDMIFYESDVLDADSNFFSFFSFKLLKGNPETCLAEPNSLVITEDMAQKYFGN
                     EDPIGKILVIGNQNTSYKVTGVSENPPHNSHFTYKALLTTVSQDQFRDDIWLNNGIHT
                     YYKLRPDATNENMPDKFEDLVIKYVGPEVQQFMGISLDQFKSSGSEYGYYSQRLLDIH
                     LKSNQEGELGVNGNITYVYIFSAIGIFILVIACINFMNLSTARSAGRAKEVGLRKTLG
                     SLRSNLIGQFLTEAVLYTIISTALGIITVYLLLPLFNLIAGKEHTIATLLNPAIMGSL
                     ATIVLVTGLLAGSYPAFYLTSFKVTEVLKGKLKSGMKSSGVRSVLVIFQFAISIILII
                     CTTLVYQQLNFVRHKNLGFDKEKVIVIQNTAPLGNNREPFKNSLLSFAEIESASFSTH
                     VIPGTTNTTVFRSQGEDIDHIMSVYSADYDHLETFGFELKEGRFFSSDFPADSTSIVL
                     NEAAVAEFGLEDPIGSEIFYSPQNQTFKVIGVLKDFNYESLRNEVGPHALFLTHADNR
                     LAIKYSGEAASTVKLIKSKWEELAPNEPFEYYFLDDKFNALFKSEQQLGELFTIFTGL
                     AIFIACLGLFGLASFMAERRTKEIGIRKALGASTPALIQLLSTEFTKLVIISFVISIL
                     PAWYFMSSWLDNFAFRIELSPLVFVLAGFGALLLAWITVGYQSIKVAGSNPVNSLRYE
                     "
     gene            9851..10741
                     /locus_tag="VGA21_08245"
     CDS             9851..10741
                     /locus_tag="VGA21_08245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650536.1"
                     /translation="MNNSALINRIFILALFTGLASCGSETKSTEDASDEFAAAEEELK
                     EKISSVIHELPPPSEVPFLLEATGADFNESLVNPIERADSYKSTSQKSALNLGIYATD
                     IGYLSSYNKTQEALNYLNASKGIADNMGITGAFDVELLKRFESNLNSRDSLASIINES
                     VGKADEYLRNDDRTKVAVMVLSGSFVEGLSIATKLVETYPKDLLPEEAKNLILTPIIR
                     VILNQEKPLTDLIDMLEDLPADSELAGLIASFRELKTIYEESDIEEKISQNQADLILS
                     GEYLTAISDKISEIRNSIVN"
     gene            10939..13986
                     /locus_tag="VGA21_08250"
     CDS             10939..13986
                     /locus_tag="VGA21_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009577491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX9650537.1"
                     /translation="MSEPLLRAIIELFAIVAKEDDVTQSERDSIEHFLLDHLNENKAK
                     EYINQFDEAANRILSFSDKNRDELKEVEKICAHINLELTFQQKVILMLNLIELILADG
                     IISDMEDKLVRKIADEFNINQADVEVITRFLIGKTATELSAEKVLIVDSAGGENYKLH
                     LPRADLRGFFAFLHLPSSGFYFVKYLGKMDIFLNGIPLKPERVRVFSLGSTLRAHHLD
                     PLFYSDIIGCFHTPEYETNIAFKTDKIWYKFRSGKIGLRGIEIEEKSGHMVGLMGASG
                     SGKSTLLNVLNGTEAPYKGSVTINGIDIHRDPEKIEGVIGFVPQDDLLIEELTVYQNL
                     YYAAKLCFDNLSEDELNKLVLKTISNLGLSETRDLRVGSPLDKTISGGQRKRLNIGLE
                     LLREPAVLFVDEPTSGLSSRDSENIMDLLKELALRGKLIFVVIHQPSSDIFKMFDKLV
                     ILDVGGYQIFYGNPIEAVIYFKEIVEMVNSQEGECIECGNVNPEQIFQIIETKVVDEY
                     GRITDERKITPQQWNRYFEKMINIEQIKEVHDPPPPSLSIPGRFQQLRIFSIRDVLTK
                     ISNKQYLVINFLEAPVLAFILAYIVRFATSQTSGEEYFFGKNVNIPAYLFMSIIVALF
                     MGLTVSAEEIIRDAKILKREAFLNLSRGSYLSSKVGILFFISAFQTLAFVIVGNSILE
                     IKGMFLQHWAILFSVSCFANLLGLNISSAFNKAVTIYILIPILLIPQLILSGVVVNFD
                     NLNPRLSAQDKVPLVGDLMASRWAYEAAMVTQFKENRYQKLFFEFDRTMGNAEYKKSY
                     YVPKLESKLDFCHTYFRSNDPDIKKDMQANLELLRLEINKELNLVGHDKLDINLLSMG
                     NFNDTSYQTLKEFLEILKKFYSNQFNNANDEKEQIINGYIEEPEKRREFENLRYSYQN
                     DRIAQVVKNMADPERIVEQDGQLIRKIYPIFLRPESNGSIASLRTPFYVPEKPFFGRY
                     LSTFQFNLGVIWLMVIILSTSLYYDWLRKILNSFTSLSYRLRR"
     gene            14002..14772
                     /locus_tag="VGA21_08255"
     CDS             14002..14772
                     /locus_tag="VGA21_08255"
                     /inference="COORDINATES: protein motif:HMM:NF014724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEX9650538.1"
                     /translation="MFFVLSKLVNILIMPLTWITATMVAALIFRKKRIGKKLLVIGIG
                     LIFLFGNEFLVNEIYLIWEEPPIEFEKIDTRYDIAIILGGVTKTNLEPRDRVHFDRGA
                     DRVVHPLFLYKMGKIDKFLVSGGTGRLINAKYSEAPSLAMALEIFGVPKEQIIIEDQS
                     RNTRENAIFSKKIIDSLYNKPPKILVVTSAFHMPRSKRCFNKINLDADFLTTDFLGHP
                     REFGLDTLLFPQVDALMKWSKLFKEWVGTIAYKVAGYI"
     gene            14825..16804
                     /locus_tag="VGA21_08260"
     CDS             14825..16804
                     /locus_tag="VGA21_08260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HEX9650539.1"
                     /translation="MKRHIQNPQTALLRMLLLYGGLTLLTLGVIIRLFPERPLDPIWM
                     RTGFVIMCFGTYFLSYKRSWVIKNLTRIILIQVFLYIIWLTYLIVINQLDTFTGAVTF
                     FSILGICLVFTNPRKLLVFLILYAVFLAPIILSIENPIIDPYIFITLFVFSGALGYLI
                     LEYIILMQKKLQRSNNDFRTLVDSIQDSIFLLSRNYRVIKVNRNGEEILQRFFSRTIK
                     EGDYILDFLFDGQRKKFQSNFDKALEGESVIVHSKLFSKDDVAYWFEFKYIPVRIEAK
                     EIRAVLFSAVDITDKKASAELMESSEKRFRTVFEAAPVGMAILTLDGFISDVNASFSN
                     TLGFRNSSVLQKDLVSLIHPEKMDYTLDHLSKLKKKEFEEFQGEMEVLRENGSYLHTI
                     TRVARISNLGKSDFLLAQIVDISQLKESEDRLKEKNLELEKANAELDKFVYSAAHDLR
                     APLTSVLGLINLVKSENGTDLRKYIDLIEKSITKLDYFIKDVIDYSRNSRLGISAEVI
                     NIKSLIEKTFEYHNFIESGDKISKQILIDGDGEFYSDQGRLEIILNNIISNAIKYFDP
                     KKKDPFIKVSATIESHGLSLEISDNGIGIPPENLDKIFNIFYRGHEQSKGSGLGLYIV
                     KETVEKINGVIMVESELNKGTSFKIRIPNTVLEDA"
     gene            complement(16801..19374)
                     /locus_tag="VGA21_08265"
     CDS             complement(16801..19374)
                     /locus_tag="VGA21_08265"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX9650540.1"
                     /translation="MEYIIKIKKKRKNLFNDWWVWKMAWMDARHNFSRLFLFISSIII
                     GIAALVAINSFRDNLHQDINDRAKDLLGADLVVSSNKTFDENTLAQFDSLKYAQAMDA
                     RMASMVLFKSEIEGTRLVQIVAVKGGFPFYGKAETLPENAWYNLDSGTYALLDENLAI
                     QYEVSTGDSVKVGSIMFPVAGVVTNLPGVTGFSSTFAPSVYISFDKLEATGLVQYGSR
                     INYNRYFKTDSDADTEELISTLRPGLRRNGYSYDDVEERKEDMGRGLDNLYRFFNLLG
                     FVALILGCIGVASSVHIYVQEKRPSVAVLRCLGASGWQSFNIFLLQALFLGMIGSFIG
                     AVFGVLIQYLIPWLFSDLIPMEININLSWLSIGEGMLLGLIISGIFSFLPLTGIRSIP
                     PLEVLRTAFEKPKRVSKIRILVIALALLFPLGFAIYQTGRIDVGLSFFAALILTFLAL
                     YAVAQLLIILLKRFFPSSWTFIWRQALANLFRPYNQTTVLMVVIGLGAFLVSTLNLVQ
                     NSLLNQVEFIGSGSRANTVLFDIQSSQREGIIELTKDHEVEILQMVPIVTMKIETVKG
                     RTVREIQEDTTDNVSNWSITREYRVTYRDSLIDSETLMAGELMPGYDRDNDDSLFVSV
                     SESMAERLGVDLKDAITFDVQGVSIKTYIGSIRDVDWQRIQTNFIFVFPLGVLEEAPQ
                     FHVMMAKIRDKEKSTGFQRQLIARFPNVSVIDLTLILKTVDDFMGKVAMVIQFMALFS
                     ISTGMVVLAGAVINSKYLRLKENVLLRTLGALRRQIVKMTLIEYAYLGLFAGLTGVIL
                     SLLAGWGLTIFFFDVVFFPDLLGLSQIWIGITMLTMLVGWVNTRGIFDRSPLEVLRKE
                     V"
     gene            complement(19358..20071)
                     /locus_tag="VGA21_08270"
     CDS             complement(19358..20071)
                     /locus_tag="VGA21_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002696798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9650541.1"
                     /translation="MPEILGVQNLVKKFSSSKQELTVIDDITFSVEEGSSIAIVGPSG
                     SGKTTLLGLCAGLDVPTSGIVSLVGIKLNSLDEDDRAYVRNQKVGFVFQNFQLLPTLT
                     AIENVMVPLELRGEKNIGKRATELMDRVGLGDRTNHYPSQLSGGEQQRVAIARAFISS
                     PQILFADEPTGNLDEETASRVTNLLFELNKSEGTTLVLVTHNVSLAENTERILHLKRG
                     KLESDQLLVQKPKNFGVHN"
     gene            20169..20783
                     /locus_tag="VGA21_08275"
     CDS             20169..20783
                     /locus_tag="VGA21_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009578222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HEX9650542.1"
                     /translation="MTLRTHLILLLVLISSFQGFQESKTILFYGDSLTAGYGLTPEQA
                     FPALVEKMLNSNGQQYRVVNAGVSGETSAGGLSRINWILNQHIDVFILELGANDGLRG
                     VPLSSTKENLQGIINQVRKKYPVVKIVIAGMMVPPNMGTEYSNEFQTMFPKLANDNDT
                     ALIPFLLDGVAGDPTLNLPDGIHPNVQGHKIVAGNVMQVLQQIL"
     gene            20905..23271
                     /locus_tag="VGA21_08280"
     CDS             20905..23271
                     /locus_tag="VGA21_08280"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9650543.1"
                     /translation="MLRNYFLVTIRNLIKNYVYSIINVSGLAIGITCSILILLWVQDE
                     LAFDKFHPKSDRLYQVWVNAEFDGNINSWRSVPLPTHMAMKDADTKIKNTAVTDWGNE
                     HLLRVNDIRIIKRGYFASDEFLEMFEFPLIQGDASVVLDDASSIVITESLAKTLFPDE
                     DPMDKIIRVDNENDVKVVGILKDVPENSTFQFDYLLPWNYYYQHSDWVKRNTTNWGNY
                     SFQVYVELDKPENEISVEQNIADLLTRNGQDDIERKFFLHPLIRWRLHSNFENGVETG
                     GMSDYVQLFTIIAIFIVVIACINFMNLATARSERRAREVGIRKSVGSTRWELILQFIG
                     ESVFIALIAFIIAIGLSQLLLPSYNTLVEKQLFIDYTNYNYWLFGIGFVLIIGLFSGS
                     YPAFYLSHFSPVKVLKGKVHIGKGASTPRKVLVTLQFSFSILLIIGTIVIYQQIQLSK
                     GRDLGYDQENLISVTYNDELAENYNVIKQELLNSGEALAVNRSNSAITQINSNNFLGW
                     PGKPEDLRVIFTTISTDYDYCKTMGIKILEGRDFSEDFKSDTLAIIINKAGLEIMGLE
                     NPIGTEFDLWGHKRTLIGIIDNVIMGSPSQPVKPMFAIMDPTWIGVVTIRLRKTNDLN
                     ASLQKVQSVFEKYNSAYPFEYKFADEEFARKFTTINLTSTLASLFAFLTILITGLGLF
                     GLASYTAEQKTKEISIRKVLGASVASLINLMSRDFSKLVIVAFIISSPISWWLMNNYL
                     ERYPIRTEIYWWIFPVTGLIALVFALTIIIAQSLKAAHANPVNSLRNE"
     gene            23429..24001
                     /locus_tag="VGA21_08285"
     CDS             23429..24001
                     /locus_tag="VGA21_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015029918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HEX9650544.1"
                     /translation="MKSFLLFVFLSSGILQDNFYSELSEAAVFRTTQPVAYDGSYFAI
                     GYPNGDIPSDKGVCTDLVIRSYRRIGIDLQQKVHEDMMANFHLYPKNWGLNGPDKNID
                     HRRVLNLMKFFSRFGEVKEITNNPIDYSFGDIVTWNLGAGITHIGIIISQKSTDGKRS
                     LVAHNIGRGPEISDCLFDFEITGHYRYRGK"
     gene            24073..24729
                     /locus_tag="VGA21_08290"
     CDS             24073..24729
                     /locus_tag="VGA21_08290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650545.1"
                     /translation="MKKIDWTHHFMQLFVVFIGITAALLLNSWRQQMNQKQLALKYYG
                     SLMRDLTFDADDFEELLPFVEHQDSILFSFLSKVNKEELDEAGIANLLQVLVSFSQFT
                     AETSTFETIKYSGNLDVFSDYELREEINSYYNKVDEVMLKQSLHYDYMNNYCIPFIYR
                     NADILAGKLLPEAELGTEFTNVAVGYQALLRQNISVYRGLMDENRSLKLRLAKKLANQ
                     "
     gene            complement(24723..26006)
                     /locus_tag="VGA21_08295"
     CDS             complement(24723..26006)
                     /locus_tag="VGA21_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009581269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEX9650546.1"
                     /translation="MYLRKMFQDNTPLAERMRPKLLGELLGQEHLVGEGSVLRQAIHS
                     GIIPSMILWGPPGTGKTTIANIIANEVKSPFKTLSAVSSGVKDVREVINKAGKGSKVI
                     LFIDEIHRFNKSQQDALLSAVEKGIITLIGATTENPSFEVNSALLSRCQVYILKPLLE
                     EHLKTLLDKCLREDYDLKDRKITIKEYKALFSISGGDARKLINLLELVTDSQSNGEVI
                     ITDEMVVNVAQQRVAVYDKSGEQHYDIISALIKSLRGSDPNAALYYLARMIEGGEDVK
                     FIARRMLILASEDIGNANPTALVMANACFQAVNVIGYPEAQLILSQCAIYLAGSPKSN
                     SATTGIGNALNIVRETGDLPVPLHLRNAPTKLMKEEGYGKGYKYSHSFPGNFAEQEFL
                     PDLISGTKFFEPGDNPREEEMRKQLRKLWKDKYGY"
     gene            26075..>26515
                     /locus_tag="VGA21_08300"
     CDS             26075..>26515
                     /locus_tag="VGA21_08300"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX9650547.1"
                     /translation="MSESFKIHGGLLTVGLIYGANYSIAKIVMPEYVDAFGLILVRVF
                     VSIILLWVIQRVWIKEWMHLTDLKRFFLCAIFGVATNQLAFFKGLSMTSPISASIIMT
                     INPVMVLIFSWLILKERVTALKVLGILLGAAGAALMISRNGIDFN"
BASE COUNT         7983 a         5329 c         5211 g         7992 t
ORIGIN      
        1 tcgaaccgct gtaaaaggtt ttgcagacct ctgcctagcc actcggccac gcgaccttaa
       61 tttatcattt ttaaacttca atatatgaga gcaacaagta agcaaaagta agcgtttctc
      121 ccttcttctt caactcctta aaaaaaatgg tagtttctca gtttggaatc attatacatg
      181 tgccacactt tttcgacatg gaaaagcaat caatttcact taataaagac ggaagtgtgg
      241 cacacgtgga tattaatttc attgtgatct taactgagac actactccct aaaaaacaat
      301 aggaggcact ttgcagcaac ctccaataaa ctaacagttt aaataaaatc ttattcctct
      361 tttttgcttt cttcagaaac tacttgctca ttattgcttt cttcgtctgt ttctgctttt
      421 ttgcttttct tcgatttacc ttctttggga gtctcactca tcttggtctt taattcactt
      481 aatgcttcga tatctcccaa agtagatttt tcaacctctt tgctcacctt ggctgccgct
      541 tttttaatcg gctttttctt ggcaggagct ttaacttctt ctttgtaggt agcgacatga
      601 gatagaagaa ttctcttttc atctttagag aaatccagaa ctttaaattc aagcgtttct
      661 cctacctccg gccgggaacc gtcttctttt gccgcattct tgttggccga aaagccttca
      721 atgccgtaag gaagttcaag caccactcct ttatccacct tttcaatcac tgtacatttg
      781 tgtacgctgc ctgtggcgaa tacatcttca aatgcttccc agggattctc ttccaattgc
      841 ttatggccaa gtgctaatct cctgttctca acatccagtt ccagaacttt cacttccaac
      901 tcttctccca ctttcacaaa ttccgaagga tgcttaatct tttttgtcca gcttaagtcg
      961 gatacgtgaa ccagtccgtc aataccttct tcaagttcaa taaataaacc gaagttggta
     1021 agattcctga caattccctt atgtgttgtt cccaccgcat atttggtaag cagatcctgc
     1081 ttggtccagg ggtcttcggt aagttgctta atgccgagtg acattttgcg ctcatccctg
     1141 tcaatggtaa taaccaccgc ttccatcgca tcacccacct tcatgaaatc ctgggggttt
     1201 ctaaggtgtt gcgaccacga catttcagac acgtgtatca atccttccac acctggcatc
     1261 aactcaagga acgcaccgta atcggcaaca ttgacaatct tgcctttaac tttggagcca
     1321 atctccaccg atttatccag cgaatcccag ggatggggag tgagttgctt cattcccagg
     1381 ctgattcttt tcttatcttc atcaaaatca agtaccacaa tctttatggt atcatcaagg
     1441 ttaagcactt cctgagggtg attgattctg ccccatgaaa tatcagtgat atgaagcaat
     1501 ccatctacac cgccaaggtc tataaataca ccaaaattgg tcatgttctt tattacacct
     1561 tcgagcactt gtcctttttc caggttattc agaatttcag ctttctgtgc ttcaagatct
     1621 ttttcaatca aaactttatg tgaaaccact acgttgtcat tcgagtaatt gatcttgaca
     1681 actttcactt ccattttctt atccacaaaa acatcaaagt cgcgaattgg cttaacatca
     1741 atttgcgaac ccggcaagaa ggcttcaata ccataaatgt caacgatcaa tccaccttta
     1801 gtacgtctct tgacaaaccc ttcaattacg gaatcttcgt caaaagcttt ttgtatctta
     1861 tcccaggcgc caactatttt agcttttctt cggcttaggt tcaactgacc agtagcgtct
     1921 tcctgttctt caatataaac ttctaccttg tccccgactt tcaaatcggg cgtatctcta
     1981 aattctgagg ctgaaaccaa tccatctgat ttgaatccaa tgttaaccac tacatcacgg
     2041 tcattgatat ccaccactgt tccggtcaca acttcttttt cagggatttg gttgaatgtt
     2101 cctgaataca atttctccag atcttccctc tctttgtctg aatagccctc gccaaaacct
     2161 ttttgatcca aggcatccca gtcaaattca ccaggtgctt ccttttgtaa ttctttcttt
     2221 gattttgacg aagaagtttt ccttaattcc tcaactgcgg atgcagcttc tgcaggttgc
     2281 tccggcgatg agtttgttgt ttccgaacct gctgaatttt cgttggtttc ctcctgctta
     2341 atttctttgt tttcctctga ttctgtcata ataaaatatg ccccaatatt ttacatcccg
     2401 ggacagcaaa aagggatatt tgttgtttaa catttccccg aaaggaaccc ctcctttgag
     2461 cggactgcaa aggtattaaa atttgtagaa aaaaaggctt tatcactaaa aaaggtagtg
     2521 tctcagctaa atgcaaatga ttttaacatc aacgtgtggc acacttgcac ttcttattgt
     2581 tggttgatag attttcaatg tcgacaaagt gtggcacacg taaaacgagc taaaaaaaac
     2641 gggggaacat gaacagtaaa aggagaccag tagtaggtct cctctatgca acggtactta
     2701 aaattatagc taattaaaac gatttcttta aggcattgat tcaagtgaca gagttcctgt
     2761 cgcataatca atcaaaattc tttgaaaaat taaccggtat ttattggatg ccaacgtggt
     2821 ttcagcctgt acaaaaagct gcatggcctg attgagttcg acctgggagg caattcccaa
     2881 ttcgtaggat tcgcgttgta cctgaacagc cagctccgcc gcctctcgtt gtgcaaggct
     2941 tgcctggtag gcattcagaa aatcctgaaa gcttctatat atattataga cttcagattt
     3001 gatttgtaat tctctgaaga ggacgtcaag ttcagcattt tcataagcca ctcgggatct
     3061 caccaccgga gtgcggttat tccagccggt aaatattgga atgtttattt gaatacctac
     3121 ttgcttgttc acattgtcag ttagaaattg gtcaccaatg ggtctgtcat ccctgtcttg
     3181 aatgttggca tatctggatg acagcccgcc aaaagcagaa actcttggca atgattgagt
     3241 gcggcttatg gcaatggttc ttttagcagc cattgcagat tggtttagac tttccaggtc
     3301 tgcccggtta tccatcgcaa tttttaccag ttcatcaaga ttaaattcag caaggttaac
     3361 tgtattaaca tccaaagttg gatcggcaag ttcaaattcc ttcaggggat ccactcccat
     3421 attcaaagca agcaccgcct tatcattgcg aagatttctt tgtgcacgca gtacttcgct
     3481 ttgtagtctt ttcaattcag catcctgcgt atacaattca acaatcgcac gctggccaac
     3541 atcaacgaaa ccttttatct gatcgtaaat agcttgctgg ttatcctggt tttctccagc
     3601 aattttcaca agttcctgat ccagtaagac ctgcagataa ttgttggtaa tggtgttaat
     3661 gatatcctgt tcagttcttc gaatcaaggt ttcctgggcc tctctggcaa gccttgcctg
     3721 gcgcattgta ttaaaattac taaagccatt aaatagcagc aggtttgcct gaacctgacc
     3781 aaacaagttc gaagactgcg tattgattac atccccggtt tgctgcaaaa aggtgtttcc
     3841 actggaaatc caggcctggc ctgtagcgga aatagttgga gcataccgta atatagattc
     3901 agtacgcaga gcattgttca ccataagttg atttaccgtc tgctgcaaag taacattgtt
     3961 cttcaacgcg taggttaatg cctgcttata gcttagttgt tcaacctgag cataggcaat
     4021 gacaacgcaa ccaataaata agctgagtag gatgaacttt ttcacaaata aataaatttt
     4081 gagtttataa attcaatgat gcttttaatt cttcggaaga aatatccgct tccacctggc
     4141 catcaacaag ccggataatt ctcttgctaa acgtggcatt cttttccgaa tgagttacct
     4201 gaattattgt cattccttct tcattcaatt ttttgaagat ttccataatc tcctcaccct
     4261 gttctgagtg gagatttccc gtaggttcat ctgcaagaag aagctttggc tgaccgatga
     4321 ttgccctggc aacaccaacc agttgctgtt gtccaccaga tagttgttca ggaaataaat
     4381 cttttttggc caccatctgg aagcggtcaa gaatttcggc aaccatgctt tttctttttg
     4441 aagaagaaac ccctttatac aaaagtgggg tttcaatatt ttcatagacc gtgagctcat
     4501 caataaggtg ataagcctga aaaatgaacc ctataaaatg cttatgcaat tcagatttct
     4561 ttcgctcttt taacttgtgc acctgctctc caaggaaatg atattcaccg gcgctggcct
     4621 catcaagcat tccaataata ttcagaaggg tggattttcc agctccactg ggtcccataa
     4681 tcgtaacgaa ctcaccttcg ttaatttgac aatcaattcc tttcagaata aaggtcctct
     4741 ggaaccttga gtcaacatac ttatcaatgt cttttaattc tatcatgtag tgttaattaa
     4801 taccaggttt ttccaagagt tagccaagaa gcatgccaag ggatatttac ctctgaatgc
     4861 ttcattttgt tcatttccag gctatttaat gcaagtcgct gtccgctacc ggagacaggt
     4921 gtccgaaaac agacacgggg ttttaattcc ttttatgaga ttgaaatttt gtcgttctta
     4981 ggcaaaaact tcgttggaat taatgaggcc gttctggaaa ttatatttaa aaatttttgg
     5041 gtggaaaatt atcgggatgc cacccagtct tgataagttc gtgataatcg ttgggccaca
     5101 taccagctat cttgacttca tattaggagt tgcagtgaga tctgttatgc aaatgaggac
     5161 tcattacctg ggaaaaaaag aattgtttca acctcccttt ggcttcattt tcaaaatgct
     5221 tggtggttat cccgttgacc gatcgagcag caagaatatg gttgatgaag tagtgaaagt
     5281 ttttgatgca cacgagaaat tttcaattgc tctggcaccg gaaggaaccc ggaaaaaagt
     5341 ggaaaaactc agaacaggat tctaccacat tgctttgaag gcaaaagttc cgttggcaat
     5401 ggctgcattg gattttggaa ataaggaagt cattttttcc gacccttttg agctaacagg
     5461 aaataaacag gaagattttg aaagaatttt tgccttcttt agaaacgtga aaggaaaata
     5521 tcccgagtca ggcatgcaca atcttaaaat tgagtagtta tgaagacaga tccaaacctg
     5581 ttagatctat ttatccattg ggaattaaaa acacctgata agcctttttt gaatgaaccg
     5641 gtttcaggag aatataaaac catcacatgg ggggaagccg gtaatcaagt ccggaaaatg
     5701 gcaaattatc tggtttcgtt aagtttaccg ccaaaatcac atatcgcaat tcttggaaag
     5761 aattcagcac actggataat ggctgatctc gccataatga tgtcaggaca tgtttccatt
     5821 cctttatatc ccaatctgac ttcagagcaa ttgaacctca ttctggaaca cagcgaatcc
     5881 aacttattgt ttattgggaa acttgatcag tttgaagaat tgaaaaaagg aattccggaa
     5941 aacatgcctt gcatttcatt tccattctat ccacatcctg gctatccaac ctgggatgac
     6001 ttgattgcgg atcaggaagg cggcgaattt tcaccccgaa ataatgatga cctttactgt
     6061 attctctata cttcaggaac aacaggtact cccaaaggag taatgcacac atttgggaat
     6121 ttcgcttttg ctatcaaagg gattaaagaa gttgttaatc taaaaaatga aacctttttt
     6181 agctatttac ccctatgcca tgttgcagaa aaaatggtaa ttgaatcagc aagcctgctt
     6241 tcagggggtg cggttttttt tgcagagtca attgacactt tcgctaaaga ccttgcctat
     6301 tcaaagccta ccttattcct ggcagtcccc cggatctgga ctaaatttca ggaaagcatt
     6361 ctggcaaaac tcccgcaatc aagattaaat cttttgcttt ctattcccat cataaataca
     6421 attttgaaaa aatcaattaa gaaaaagttg ggcttgaccc gggcgagaat aatttttagt
     6481 ggagccgccc caatctcaac ttcacttctt gaatggttta agaaattagg aatccacatt
     6541 caggaagcat acggaatgac tgaaaatctc tcctattcac atttcaacag accaggtgct
     6601 ataagaattg gaagtgttgg tcaggcgttg cccagatgcg aagttaagat aagtgagcaa
     6661 tcggaagtgc tggttaaaag tgaggccact atgaggggtt acttcaaaga ccaggaacaa
     6721 actaatgaga tgattacgtc agatggtttt ttgaagaccg gtgatgaagg cagaatagat
     6781 gaggaaggat tcctttacat caccggcagg gttaaagaca ttttcaaaac atccaaagga
     6841 aaatacattg cgccggcccc gattgaaaag atgatcatgt cttatgaatt ggtttcgcaa
     6901 acctgtgttg ttggctccgg acttccacag ccaatagccc ttattactat tcctggtaaa
     6961 ggaacggata ttcaggaaaa tgttattgat catttcaaaa caatattgga agaaagtaat
     7021 gaaaagctcg cggcacatga acagctgcga aaaattgttc ttttcaggca ggaatggacc
     7081 gtcagggata ttctaacgcc ttctctgaaa ataaaacgcc gaagtgtgga agcaatttat
     7141 ggggcaaggt tcgagagttg gtatgaagat gctgaagatg tgatttttgg ctgagagagt
     7201 acccctgcca gaccaaaaat ctatttagat gttcaaccta ttcgtatcga agggaattca
     7261 caggattaga accggccact tttatggatt ggtaaccaac tgtaatccaa gccaataaca
     7321 gggcaccaaa tcctgccaaa acaaaaacca aagggctcag ttcaatccgg aatgcaaaat
     7381 tatccagcca ggaactcata aaataccagg ctggcaatat gctaatcaca aaggaaatta
     7441 ttaccagctt tgtaaattct gtcgaaagca attgtatgag agctggggta gaagcaccca
     7501 gagcttttct aattccaatt tcctttgtac gtctttcagc catgaaagat gccagaccaa
     7561 acaggcccag gcatgcaata aaaatggcta agccggtgaa aatcgtaaac aattcaccca
     7621 gttgttgttc tgatttaaat agcgcattga atttatcgtc aaggaaatag tattcgaagg
     7681 gctcatttgg tgctaattct tcccattttg atttaattag tttaaccgtg ctcgccgctt
     7741 caccggagta tttaattgcg agcctgttat cagcatgagt taaaaacaac gcgtgcgggc
     7801 caacttcatt tcttaaagac tcataattaa aatcctttaa aacacctata accttaaaag
     7861 tttgattttg cggtgaataa aaaatttccg aacctattgg atcttcaaga ccaaattctg
     7921 ccacagctgc ttcgtttaat actatcgaag tggagtcagc aggaaaatcg cttgaaaaga
     7981 accttccttc ctttaattca aatccgaatg tttccaggtg gtcataatct gcactatata
     8041 ctgacattat gtggtctata tcttctccct gggatctgaa aactgtagta ttcgtagtgc
     8101 ccggaataac atgggtggaa aaacttgcac tttcaatttc ggcaaaggat aataatgaat
     8161 tcttaaaagg ttcacgatta tttccaagag gcgcagtatt ttgaataacg attactttct
     8221 ctttatcaaa tcctaaattt ttatgtcgta caaaatttaa ttgctggtaa acaagggtag
     8281 tgcatattat gagaattatg gagattgcga attgaaagat tactaaaacc gaccgaactc
     8341 cgctactctt cattccggat ttcaattttc cttttaaaac ctcagtaact ttgaatgagg
     8401 ttaaatagaa cgcaggataa cttccggcta ataatccggt aacaagaaca atggttgcca
     8461 aacttcccat aattgccggg tttaggaggg tggcaattgt atgttcttta cccgcaatta
     8521 agttaaataa aggcaataaa agatacaccg taataatacc caatgctgta gaaattatgg
     8581 tatacaaaac agcttcggtc aggaactgac caataaggtt gcttctcaaa ctgccgaggg
     8641 tctttcttaa acccacttcc ttagccctgc cagcagatct tgctgttgaa agattcataa
     8701 aatttataca tgcaattaca agaataaaaa ttccgatagc cgagaagata taaacgtagg
     8761 tgatattgcc attaacaccc aactcccctt cctgattgga ttttagatga atgtccaata
     8821 agcgttggga ataataacca tattcactac ctgagctctt aaactggtcc aaactgatac
     8881 ccatgaattg ctgcacctcc ggaccaacgt atttaataac caggtcctca aacttatctg
     8941 gcatattttc attggttgca tctggtcgaa gtttatagta agtgtgtatt ccattgttaa
     9001 gccagatatc atctcgaaac tggtcttgag atactgtggt taataatgcc ttgtaggtaa
     9061 agtgtgaatt atgaggagga ttttcagaaa cgccggttac tttgtatgat gtattttggt
     9121 ttccgattac cagaattttt cctatcggat cttcattccc aaaatatttt tgtgccatgt
     9181 cttctgttat caccagggag tttggttcgg ccaaacatgt ttcgggattt cccttcaata
     9241 atttgaaaga aaagaatgaa aagaaatttg agtctgcatc aagaacatcg gattcataaa
     9301 atatcatatc ctcataccgg aatagtattt caagattttg tcccaatcga accgcgcttt
     9361 ccacttccgg gaattcattc gccatcgcat tagcaagcgg gggacaggtg taaataacat
     9421 taatttcctg accggcaatt ttcccttgca atcctacccg gtaaattctt tttgcatccg
     9481 tatgaaactt gtcgtaggaa agctcatcta ccacataaaa aaggataaaa atagacccgg
     9541 caatgccaat tgtcaggccc atgatgttaa taaatgaata aagcttttga ttggcaatat
     9601 tccggaaagc gattttcaga tagtttttga ccattggggg tgattaattt aatctttaga
     9661 cgaccacaat gactgctttg ttacaccgct ctggcgagtg gaattgggtg acagataaat
     9721 ggttcttttt tcatattaac ttagataaaa ttgtacaata acattaaatt gagcctttat
     9781 ctaattgcca atctttggac aataatcaga taatagtatc ttgcgacaaa accgaaactt
     9841 atcaatagaa atgaataata gcgccttaat caatcgaata tttattttgg cccttttcac
     9901 cgggcttgcc agttgtggat ctgagacaaa atcgacagaa gatgcttctg atgaatttgc
     9961 cgctgccgaa gaagagttaa aagaaaaaat tagctctgtg attcatgaac tgccacctcc
    10021 atctgaagtt ccgtttcttt tggaggcaac cggggctgat tttaatgaaa gtcttgtaaa
    10081 tccgattgaa agagcagatt cctataaatc gactagtcaa aagtctgccc tcaatctcgg
    10141 aatttatgca acagatattg gctatttaag cagttacaat aagacgcagg aagccctgaa
    10201 ttacctgaat gcctctaagg gtattgcgga taatatgggc ataaccggcg cgtttgatgt
    10261 tgagctatta aagagatttg aaagcaattt gaacagcagg gattcgctgg caagcattat
    10321 caatgaatca gttgggaagg cggatgaata cttgaggaat gatgatagaa ccaaggttgc
    10381 cgttatggtt ttgtcaggaa gttttgttga aggtctgtcc attgcaacga agcttgtcga
    10441 aacatatcct aaagacctgt tgccggaaga agccaaaaac ctcatcctca ctcctattat
    10501 cagggtgatt ctgaatcagg agaaaccact taccgacctt attgacatgt tggaggattt
    10561 gccggctgat agcgagttag ccggattgat tgcttcgttt agggaattga aaactattta
    10621 tgaggaatct gatatagaag agaaaatttc tcagaatcag gcggacctta ttctaagtgg
    10681 tgaatatcta accgcaatta gtgacaaaat ttctgaaatc aggaacagca tcgtaaacta
    10741 gcctaatcta cctgctatat ataaattggc ccgtggtacg ggcctttttt ttctttaaag
    10801 atgccaaaat gaattttgtc accgggaaca agaatttgtg aattctctta attcacgaag
    10861 gtttgtaacg ggtgaattta gctaaaatta aattagcgcc ttatatttag acaatactaa
    10921 actcccctat caacccaaat gagtgaacct ttattgcgtg ctataattga actttttgct
    10981 attgtagcca aagaagatga cgttacccaa agcgagcgcg attccatcga acatttcctg
    11041 ctggatcatc ttaatgaaaa taaagccaag gaatatatta atcaatttga tgaagctgca
    11101 aacagaatat tgagcttctc cgacaagaac cgggatgaat taaaagaggt tgaaaaaatt
    11161 tgcgcgcata ttaatcttga gcttaccttt caacaaaaag ttattttaat gctaaacctg
    11221 attgagttga ttctggcaga cggaataatt tccgatatgg aagataaact cgtcagaaaa
    11281 attgcagatg aattcaacat taatcaggct gatgtggaag ttatcaccag gttcctgata
    11341 ggtaaaaccg ctactgagct gtccgctgaa aaggtattga ttgtagattc ggccggtggt
    11401 gaaaattaca aattgcattt gccgcgcgca gacctccgcg gcttcttcgc atttttacat
    11461 cttccatcca gtggtttcta ttttgttaag tatttgggaa aaatggacat tttcctgaat
    11521 ggcattcccc tgaagcctga aagggtgaga gttttttcgc ttggcagcac cctgagagct
    11581 caccatcttg atccgctatt ttatagtgat attatcggat gctttcatac tcccgaatac
    11641 gaaaccaaca ttgcgtttaa gaccgataaa atctggtaca aattcagaag tggcaaaatc
    11701 ggactgcgcg gaattgaaat tgaagagaag tcgggccata tggttgggct tatgggcgcc
    11761 agcggctcag gcaagtctac tctgctgaat gtactaaatg gcactgaagc tccctataag
    11821 ggaagtgtta ccatcaacgg gattgacatc cacagggatc ctgaaaagat tgaaggagta
    11881 attggatttg taccgcagga tgatttgctg attgaagagc tgacggtgta ccaaaatttg
    11941 tattatgcgg ctaagttatg ctttgacaat ttaagtgaag acgagttaaa taaactggtg
    12001 ctaaagacca taagtaattt agggctttca gaaaccaggg acttgcgggt gggttctcca
    12061 ctggataaga caatctcagg tggccagcgt aaaagactaa atataggact ggagttatta
    12121 agggagccgg ctgtactttt tgtcgatgaa cctacttcag gattgtcgtc aagggattct
    12181 gaaaacataa tggatctcct caaggaatta gccttacggg gtaaactaat ctttgttgta
    12241 attcaccagc catcatcaga tattttcaag atgtttgata agctggttat tctagatgtt
    12301 ggcggctatc agatttttta tggcaatcca attgaagctg taatttattt caaggaaatc
    12361 gtggaaatgg ttaacagcca ggaaggagaa tgcattgagt gcggcaatgt aaatcctgaa
    12421 cagattttcc aaataattga aaccaaggta gttgatgaat acgggagaat aacagatgag
    12481 cgtaaaatta cgcctcagca atggaaccgt tattttgaaa aaatgatcaa cattgagcaa
    12541 atcaaagaag ttcatgaccc acctccacct tctttaagca tcccgggaag gtttcagcaa
    12601 ctgagaattt tcagtatccg ggacgtcctc accaaaatca gcaataagca atatttggtg
    12661 atcaattttc ttgaagcacc ggttctcgct ttcattctgg cttatattgt aagattcgcg
    12721 acatcacaaa ccagtggaga agaatatttt tttggaaaga atgtaaatat acccgcttac
    12781 ttattcatgt caataattgt tgcattattc atgggactga ccgttagtgc ggaagaaatt
    12841 attcgtgatg caaaaatcct gaaaagagaa gcctttctta atttgagtcg tggaagttat
    12901 ctttcctcca aagtcggtat tctatttttc attagcgcgt ttcaaactct cgcatttgtg
    12961 attgtgggaa attctatttt agaaatcaaa ggaatgttcc tccagcattg ggcaattctt
    13021 ttctcagttt catgttttgc caatttattg ggtctcaata tcagcagcgc tttcaacaaa
    13081 gcggtaacta tttacatttt gattcctatt ctgcttatcc cccagctaat attaagcgga
    13141 gttgttgtaa actttgacaa tcttaacccg cgcttgagtg cgcaggataa agttcctctt
    13201 gtcggtgatc tcatggcaag ccgatgggct tacgaggccg cgatggttac tcaatttaaa
    13261 gagaacagat atcagaaact tttttttgaa ttcgacagga ctatgggcaa tgcagagtat
    13321 aagaagtctt attatgttcc aaaactcgaa agcaaacttg atttttgtca cacctatttc
    13381 aggtctaatg atccggacat aaaaaaggat atgcaagcca accttgaatt actcaggttg
    13441 gaaattaaca aagaacttaa cctggttggt catgacaaac ttgatattaa cctgctaagc
    13501 atggggaatt tcaatgacac ttcttaccaa acactgaaag aatttctgga aatactaaaa
    13561 aagttctatt caaatcagtt taacaatgct aatgacgaga aggaacaaat aattaatggt
    13621 tacattgagg agcctgagaa acgcagagaa tttgagaatc ttcggtatag ttatcaaaat
    13681 gaccgaattg cccaggtggt gaaaaatatg gctgatcccg aaaggatcgt ggaacaggat
    13741 ggccaattga tcaggaaaat atatccgatt tttctgcgac ctgaaagcaa tgggtctatc
    13801 gcaagcttaa gaacaccgtt ttacgttccg gagaagcctt tttttggaag gtatctatca
    13861 acatttcaat tcaatttagg ggttatttgg ttaatggtaa ttattctttc aactagcctt
    13921 tactatgatt ggttaagaaa aattttaaac tcatttacca gtttatctta ccgattgaga
    13981 cgatagaaaa ttcaaacaca gatgtttttt gtactttcca aattagtcaa tatcctgatc
    14041 atgccgctta cctggataac cgcaacaatg gtagcagcgc taatctttag aaaaaagcga
    14101 attggcaaga aattgttggt tatcggtatt ggacttattt ttttatttgg aaatgaattc
    14161 ctggtaaatg aaatttatct gatctgggaa gagccgccaa ttgaatttga gaaaatagac
    14221 acgcgctatg acatcgcgat aattctaggt ggtgtaacaa aaactaatct tgaaccaaga
    14281 gacagagttc actttgatcg gggagccgat agggtggttc accctttgtt tctttataag
    14341 atgggtaaaa ttgataagtt tttggttagt ggaggtacag gaagattaat taatgcgaag
    14401 tattccgagg ctccttcact tgcaatggca ttggaaattt ttggcgttcc aaaggagcaa
    14461 atcataattg aagatcaatc cagaaacacg cgggaaaatg cgatcttttc aaaaaaaatc
    14521 attgacagct tatataacaa gccaccaaaa attttagtgg taacctccgc ttttcatatg
    14581 ccccgctcaa agcgctgttt caataaaata aatttggatg ccgatttcct taccacagat
    14641 tttctcggac atccccggga atttggcctg gatacactct tgttcccaca ggttgacgca
    14701 ttgatgaaat ggagtaaact gtttaaggaa tgggtgggaa ccattgcgta caaagtagcc
    14761 ggttatattt agggaatccg cttcttttta caatttcaat tcagtaaatt catttctcaa
    14821 aattgtgaag cgccatattc aaaatccaca aactgcattg ctcagaatgc tgctccttta
    14881 cggtggactg actttattga cattgggagt gattatcaga ctttttccgg agcgacccct
    14941 ggacccaatt tggatgagaa ccgggtttgt gataatgtgt tttggtactt atttcctctc
    15001 ctataagaga agttgggtta ttaaaaattt aacccgtatc attttaatcc aagtatttct
    15061 atatataatc tggctaactt atctgattgt aattaaccaa cttgacacat ttaccggggc
    15121 ggtaactttc ttttctatcc ttggaatttg cctggtgttt acaaacccaa gaaaattgct
    15181 tgtttttctg attttatatg ctgttttctt ggccccaatc atcctgagca ttgaaaaccc
    15241 cattatagat ccctatatct ttatcacatt attcgttttt tcaggagccc tgggatatct
    15301 cattctggaa tatataattc taatgcaaaa aaaactgcag cgcagcaaca atgacttcag
    15361 aacattagta gacagcattc aagattcaat cttcctgctt tcccggaatt acagagtaat
    15421 aaaagtgaac cggaacggag aggagattct tcagcgattt ttttccagaa ccataaaaga
    15481 aggagattat atccttgatt tcctgttcga tgggcaaaga aaaaaatttc agtcgaattt
    15541 tgacaaagca ctcgaaggag aaagcgtaat tgttcattca aagttattca gtaaagatga
    15601 cgttgcttac tggtttgaat tcaaatatat ccctgttcga attgaggcta aagaaatccg
    15661 tgcagtgctt tttagtgctg ttgatattac cgataaaaag gcttcagcgg aattaatgga
    15721 aagctcggag aagaggttca gaactgtctt tgaagccgcc cctgttggaa tggctatcct
    15781 aacgctggat ggatttattt cagatgtaaa tgcatcattc agcaatacat tgggtttcag
    15841 aaattcaagt gttttacaga aagacctggt ttcactaatc catccggaga agatggatta
    15901 tacgcttgat catttaagca agttaaagaa gaaagaattt gaggagttcc agggagagat
    15961 ggaagtgttg cgtgaaaacg gtagctacct gcacacaata acacgagttg ccaggatttc
    16021 caacctggga aaatctgatt ttctactcgc tcaaattgtt gatatcagtc agctaaagga
    16081 aagtgaagac agactaaaag aaaaaaatct tgaattggaa aaggcaaatg cggaacttga
    16141 caagttcgtt tatagcgcgg ctcatgattt gagagcgcct ctcacctcgg tacttggctt
    16201 aattaatctg gttaaatctg aaaatggcac cgacctgaga aaatacattg atcttataga
    16261 aaaaagtatt actaagcttg attattttat aaaggatgtt attgattatt caagaaacag
    16321 ccgattgggc atatccgccg aggtgatcaa tattaaatca ctgattgaaa aaacatttga
    16381 gtaccataac tttattgaaa gcggagataa gatcagcaaa caaattttga ttgatgggga
    16441 tggagagttt tatagcgatc aggggcgact tgaaattatt ctcaataaca taatatccaa
    16501 tgcaatcaag tattttgatc caaaaaagaa ggatcccttt ataaaagtat ctgcaacaat
    16561 tgaaagccat ggactgagcc ttgagatttc agacaacgga attgggattc ctccggaaaa
    16621 tcttgataag atatttaata ttttttaccg ggggcacgag caatcaaaag ggtcaggtct
    16681 tggcctttat attgttaaag agactgttga aaaaataaat ggagttatta tggtagagtc
    16741 agagcttaac aagggcacat cattcaaaat aagaattcca aataccgttc tggaggacgc
    16801 ttaaacctcc ttacgtaaaa cttccaatgg ggagcgatca aaaatcccgc gggtatttac
    16861 ccagcccacc agcattgtca acatcgtaat gccaatccag atttgtgata acccaagcaa
    16921 atccggaaag aaaacaacat cgaagaaaaa tatagttaat ccccagcctg caagtagtga
    16981 aagaataact ccggttagtc cggcaaacaa gcccagataa gcatactcaa tcagcgtcat
    17041 tttaacaatt tgtctcctca gagcgccaag tgtccggagc aacacatttt cttttaaccg
    17101 taaatacttg ctgttaatta ccgcgccggc cagcacaacc atccctgtag aaatactaaa
    17161 gagtgccatg aattgaatta ccatggccac cttgcccata aaatcatcaa ccgtttttag
    17221 aataagtgtc aagtcaataa cacttacatt cggaaatcgg gcgataagct gacgttggaa
    17281 cccggttgat ttttctttat ccctgatctt ggccatcata acatgaaatt gcggcgcttc
    17341 ttccagaaca cccaggggga atacgaaaat gaaattggtt tgaattcttt gccaatcaac
    17401 atcgcgaatg cttccaatgt atgtcttgat cgaaactccc tgtacatcaa aagttattgc
    17461 gtctttcaga tctacaccaa gtcgttcggc catgctttca gataccgata caaacaaaga
    17521 atcatcatta tccctatcat atccgggcat caactctccg gccataagcg tttccgaatc
    17581 gattaatgaa tccctgtagg taacccggta ttcgcgcgtt atactccagt tagaaacatt
    17641 gtcggtggta tcttcctgta tttcccgcac agttctcccc tttacagttt ctatcttcat
    17701 cgtaactatc ggcaccattt gtagtatttc tacttcatga tcttttgtaa gctcaataat
    17761 tccttcacgc tgactacttt ggatatcgaa taatacggta tttgcccggc tgcctgagcc
    17821 gataaattcc acctgattca ataaactatt ctgaactaaa ttcaacgtac ttaccaggaa
    17881 ggcacccagc ccgattacga ccatgagcac ggtagtctgg ttgtagggtc tgaaaagatt
    17941 ggcaagagcc tgacgccaaa tgaaagtcca cgatgatggg aaaaacctct ttaacaggat
    18001 tattaatagt tgagccacag cgtatagagc caggaaggtt agaatcaagg cagcaaaaaa
    18061 acttagccct acatcaatcc ggccggtttg ataaatagca aatcccaaag ggaaaagcag
    18121 cgctaatgca attaccagga ttcggatttt actaactctt ttaggttttt caaacgcggt
    18181 tctcagtact tccagtggtg gtattgaacg aatcccggtt aaaggcagaa atgagaaaat
    18241 accactaata atcaatccca ataacatgcc ctcacctatg gatagccaac tgaggttaat
    18301 atttatctcc atgggtatca ggtctgagaa aagccagggg attagatatt gaatcagcac
    18361 cccaaacacg gcaccaataa aacttccaat catccccagg aataaggcct ggagcagaaa
    18421 aatgttaaac gattgccatc cgctggctcc gagacatcga agtacagcca ctgaggggcg
    18481 tttctcctga acataaatgt gcaccgaact tgccactcca atacatccaa gtatcagggc
    18541 aacaaatccc aacaaattga aaaatcgata caggttatcc agtcctcttc ccatatcttc
    18601 ttttctttct tccacatcat cgtaggaata cccattacgt cgaaggcctg gtcttaaggt
    18661 agaaatcagt tcttcagtgt ctgcatcact atcggtttta aaatacctgt tataatttat
    18721 gcgtgagcca tattgaacaa gacccgtggc ttcaagttta tcaaaactta tatataccga
    18781 tggcgcaaaa gtggaactga atccggttac tcccggaagg tttgtaacta cacccgccac
    18841 cggaaacatg atacttccca cctttaccga atcaccggtg ctgacttcat actgaatggc
    18901 aaggttttca tccagcaatg cataagtgcc tgaatccaga ttataccagg cattctccgg
    18961 taaagtttcc gcctttccat aaaaaggaaa cccgccctta acagcaacaa tttgcacaag
    19021 gcgcgttcct tcgatctcgc tcttaaacag aaccatagag gccatacggg catccattgc
    19081 ctgagcatat ttaagagaat caaactgggc cagggtgttc tcatcaaaag tcttgttgga
    19141 agaaactacc aggtctgctc caagtaaatc ctttgcccga tcattgatat cctgatgaag
    19201 attatcccga aatgaattga tcgcaactaa tgccgctatt ccaattataa ttgaggaaat
    19261 aaaaaggaat agtctggaaa aattatgtct ggcatccatc catgccattt tccagaccca
    19321 ccaatcattg aagagattct tccgcttctt ttttatttta attatgtact ccaaaattct
    19381 ttggcttttg aactaatagt tgatcacttt ctaattttcc acgcttcaga tgtaaaatac
    19441 gttctgtatt ttcagcaagt gacacattat gagttactaa tactaaagtt gttccttccg
    19501 atttatttaa ttcgaagagc aggtttgtta ctcttgaggc ggtctcttca tccaggttgc
    19561 ctgtgggttc atcagcgaac aaaatctgtg gtgatgaaat gaatgcccgc gcgatggcca
    19621 cacgttgctg ttcaccacct gagagctggg aagggtagtg attagtcctg tcacccagcc
    19681 ctactctgtc cataagttca gtagcgcgtt ttccaatatt tttttcacca cgtaattcaa
    19741 gtggaaccat tacattttca atcgccgtca gggttgggag taactgaaaa ttctgaaaaa
    19801 caaatcccac tttttggttt ctcacatagg ctcgatcgtc ttcatccaaa gaatttaatt
    19861 ttatgcctac taaagaaaca attccggaag taggtacatc aagacccgcg cataatccca
    19921 gcaaagttgt tttaccgctg ccagagggac caacaattgc aattgatgat ccttcttcga
    19981 cggaaaaagt aatatcatca attactgtta gttcttgctt tgaagacgaa aacttcttta
    20041 ctaaattttg aactcctagt atttccggca taattttagt ttctggcgat actaaactga
    20101 aacattattg tagaaataaa gttttaacta atgtctaagg tagctgattt atgacaaaaa
    20161 tgtttaaaat gactctccga acccatttga tattacttct agtactgatt tcttccttcc
    20221 agggtttcca ggaatcaaaa actattttat tctatggaga tagtttgaca gcaggctatg
    20281 gactcactcc ggagcaggca ttcccggcgt tggttgaaaa gatgctcaac agcaacggtc
    20341 agcagtacag ggtggttaac gcaggagtaa gtggcgaaac ctctgccggc ggtttaagca
    20401 ggataaattg gatcctgaat cagcatattg acgttttcat tcttgaactt ggcgccaatg
    20461 atggattacg cggagtacca ctgagttcaa ccaaagaaaa cctccaggga ataattaatc
    20521 aggttaggaa aaaataccct gttgttaaaa ttgttattgc aggcatgatg gttcctccaa
    20581 atatgggcac tgaatattct aatgaatttc aaaccatgtt tccgaagttg gccaatgaca
    20641 atgatactgc tttgattcca ttccttttgg atggagttgc aggagatcca accctcaacc
    20701 ttcctgatgg aattcacccg aatgttcaag ggcacaagat tgtagccggt aacgtgatgc
    20761 aggtacttca gcaaatcctt tagtaaattt tttacagact gatacaactc ttccaattaa
    20821 ggcgggtctt tcagggaaag tttccctgat tctgtaacta aacgccagaa atcaggtata
    20881 ataagtaaca ataactaaat tcctatgctt agaaattact tcctggttac aattcgtaac
    20941 ctcattaaga attacgttta ctcgattatc aatgttagcg gactcgcaat tggcatcacg
    21001 tgtagtatct taatattgct ttgggtacag gatgaacttg cttttgataa atttcatcct
    21061 aaatcagacc ggctttacca ggtgtgggta aatgcagaat ttgatggaaa cataaattca
    21121 tggcgatcgg ttcccctgcc cactcatatg gcaatgaagg acgctgacac aaagattaag
    21181 aacaccgcgg taactgattg gggaaacgag caccttctta gagttaatga tatcagaatt
    21241 attaaacggg gttattttgc ttctgatgaa tttcttgaaa tgttcgaatt tccattaatt
    21301 cagggagatg cttcagtggt tttggatgat gcttcatcga tagtaatcac agaatccctg
    21361 gcgaagactt tgtttccgga tgaggatcca atggataaaa tcatccgggt tgataatgaa
    21421 aatgatgtaa aggttgttgg aatattaaaa gacgttcctg aaaattcaac ttttcaattt
    21481 gattatcttc tcccctggaa ttactattac caacacagtg attgggtaaa acgcaatact
    21541 acaaattggg gaaattattc gtttcaggtt tatgtggaat tggataagcc tgaaaatgaa
    21601 atctccgttg agcaaaacat tgcagatttg cttaccagaa atgggcagga tgatatagag
    21661 agaaaattct ttttacatcc attaatccgc tggaggctgc attcaaattt tgaaaatggt
    21721 gttgaaacag ggggcatgag cgattatgtt caattgttta cgatcatcgc catatttatt
    21781 gtggttattg cctgtattaa tttcatgaat cttgcaacgg caagatctga aagaagagca
    21841 cgtgaagtgg gaattagaaa gagcgtaggt tcaacgagat gggaattgat tcttcaattt
    21901 ataggagaat ctgtatttat tgctttgata gcattcatca tagcaattgg tctttctcaa
    21961 ttgcttctgc cctcttacaa tactcttgtg gaaaagcaat tattcataga ctatacaaac
    22021 tataattact ggttatttgg aataggcttt gttctcatta ttgggttatt ctccggaagt
    22081 tacccggctt tctacttatc acacttcagt ccggtgaaag tattgaaagg aaaagttcat
    22141 atcggaaaag gtgccagtac tccacgcaag gtgttggtta cgctgcagtt tagtttttcc
    22201 atactgctga taatagggac aattgttatc tatcaacaaa ttcaattatc aaaaggaaga
    22261 gaccttgggt atgaccagga gaatttaatt tccgtgactt ataacgatga gttggcagaa
    22321 aattataatg tgattaagca ggaacttctg aattcaggag aagcactggc cgtcaatcgt
    22381 tccaatagtg caattaccca gataaattcc aacaattttc tgggttggcc gggtaaaccg
    22441 gaagatttgc gggtaatttt tactaccatc tccaccgatt atgattactg taagacaatg
    22501 gggatcaaaa ttctggaagg ccgcgacttt tctgaagatt tcaaaagtga tacattagcc
    22561 ataataataa ataaggccgg attggaaatt atgggccttg aaaatcccat tggaactgaa
    22621 tttgatttat ggggtcataa gcgaaccctt attggtataa tagataacgt tatcatgggc
    22681 tctccttccc agcccgttaa accaatgttt gccataatgg atcctacctg gataggcgtg
    22741 gtaactatac gacttagaaa aaccaacgac cttaatgcct ctttgcaaaa agtgcaaagt
    22801 gtttttgaaa aatataactc agcctatccg tttgaatata aattcgcaga tgaagaattc
    22861 gcgcgtaaat ttacaactat aaatttaacc agcacactag cgagcttatt tgcttttctg
    22921 acaatcttaa tcaccggcct gggattattt gggcttgctt cttacacagc cgaacaaaaa
    22981 acaaaagaga tcagtattcg aaaagttctt ggcgcttcag tagcaagttt aataaacctg
    23041 atgtcaaggg atttttctaa gctggtcatt gtcgcattta ttatcagctc tccaatttca
    23101 tggtggctta tgaataacta tctggaaaga tatcccatcc gcacagaaat ttactggtgg
    23161 atcttcccgg ttaccggatt aattgccctg gtttttgctc taacaataat tattgcacaa
    23221 tcgctgaaag ctgcacatgc caacccggtt aattcactta ggaatgaata aaagaaacca
    23281 cggataacta gtttgttttt gattttacgg gatggcctga gagatgctga cgcatgctcc
    23341 ttgattcaac ttcagagtat aaactttaat aacctaaact aaacttatta aagaaacgtt
    23401 gtaatctgga gaaataaaca tctccaaagt gaaatctttc ctcctttttg tcttcctatc
    23461 ttccggaatt cttcaggata atttctattc tgaactttcc gaagccgcgg tattcagaac
    23521 cacgcaacct gttgcctacg atggttccta ttttgcaata ggatatccta acggagatat
    23581 tccttcagat aaaggagtgt gtacggatct ggttattcgt tcataccgcc ggataggaat
    23641 tgatttgcaa cagaaagttc atgaagacat gatggctaac tttcacctat accctaagaa
    23701 ctggggatta aatggtccgg acaaaaatat tgatcacagg cgtgtgctaa acctgatgaa
    23761 gtttttcagc aggttcggag aagtgaaaga aattacgaat aatcccattg attatagctt
    23821 tggggatatt gtgacctgga atctcggtgc aggtataacc catatcggta ttatcattag
    23881 ccaaaaatca actgatggaa aaagatctct ggtcgcgcat aatatagggc gcggaccgga
    23941 aatttccgat tgcttatttg attttgaaat caccggtcac tatcggtacc ggggaaaata
    24001 attatcccat aaaaagaata gagctaaatt ctgaaaattc ttaattttat tggattttca
    24061 aatttctcat ccatgaagaa aatagactgg actcatcatt tcatgcaact attcgtggtt
    24121 tttataggta tcactgccgc gcttctcctg aatagctgga gacaacaaat gaaccaaaaa
    24181 caattggcat taaagtatta cggcagttta atgagagatt taacttttga tgcagatgat
    24241 tttgaagaac tgcttccttt tgttgagcat caggattcga ttctttttag ttttctttca
    24301 aaagtaaata aggaagaact ggatgaagcc ggcattgcca atctgttgca agtattggtt
    24361 tcatttagcc agttcacagc agaaacttcc acttttgaaa cgattaaata tagtggcaat
    24421 ctggatgtat tttcagacta tgaactacgg gaagaaatta actcgtatta taataaagtg
    24481 gatgaagtga tgctcaaaca aagtctccat tatgactata tgaataatta ttgtataccc
    24541 ttcatatacc gaaatgcaga tattcttgcc ggaaaattgt tgccagaagc tgaattggga
    24601 accgaattta ccaatgtagc agttggttat caggctttgt taaggcaaaa catcagcgtt
    24661 taccggggat taatggatga aaataggagt ttaaaactac ggcttgcaaa aaaacttgca
    24721 aatcaataac catatttgtc cttccataac ttccgcaact gcttgcgcat ttcctcttcg
    24781 cgcgggttat ctccgggttc aaaaaatttt gttcctgaaa tcaaatccgg taaaaattcc
    24841 tgttcagcaa aatttcccgg aaatgaatga gagtatttat accctttacc atatccttcc
    24901 tctttcatca atttggtggg agcattgcgc aaatgcagag gcaccggtaa gtcgcctgtt
    24961 tcacgaacta tgttcagcgc atttccaata ccggtagttg cagaattact tttaggtgaa
    25021 cctgccaggt aaatcgcgca ctggctcagg ataagttgtg cctccggata acctattaca
    25081 tttactgcct gaaagcaggc attagccatc accaatgcag ttgggtttgc gtttccaata
    25141 tcttccgatg ccagaatcaa cattctgcgg gcaataaatt ttacatcttc accaccctct
    25201 atcatccttg ccaggtagta taatgccgca ttgggatcac ttcctcttaa agactttatg
    25261 agggctgaga taatatcata atgctgctcc ccgctcttgt cgtaaaccgc cacgcgctgt
    25321 tgggcaacat tgacaaccat ttcatcggta attattactt caccattcga ttgagaatcc
    25381 gtaaccagtt ctaaaagatt tatcaatttt ctggcatcgc cgcccgaaat tgaaaataat
    25441 gctttgtatt ctttgatggt aattttacgg tcttttaaat cataatcttc acgcaggcat
    25501 ttatctaata aagttttcag atgttcctcc agcaagggtt tcagtatgta aacctggcaa
    25561 cgtgaaagca gagcagagtt tacttcaaaa gaaggatttt cagtggtcgc gccaattaga
    25621 gtaattatac ctttctccac agcgctcaaa agtgcatcct gctgggattt gttaaacctg
    25681 tggatttcat caataaataa aattactttg ctgccctttc ctgccttatt aattacttcg
    25741 cgaacatctt taactccgga actaacggcg cttaaggttt taaacggtga ttttacctca
    25801 ttggcaatga tatttgcaat tgtagttttt cccgttccgg gcggccccca taaaatcatt
    25861 gaggggataa ttccggaatg aatggcttgc ctcaaaacac ttccctctcc aacaaggtgt
    25921 tcctgaccta ataattcccc cagcagttta ggccgcattc tttcagctaa tggtgtgtta
    25981 tcctgaaaca tctttctaag atacataaaa gaataaaacg tccggaatgt tgggttcaac
    26041 atttcaaatt gaacaagtta tatttgcccc ttcgatgagc gagagtttca aaattcatgg
    26101 aggcctgcta actgtgggtt tgatttacgg tgccaattac agcattgcaa aaattgtcat
    26161 gccggaatat gtggatgctt tcggactcat cctggtacgg gtttttgtct ccattatttt
    26221 actttgggtc attcagcggg tctggatcaa ggagtggatg catttgactg acttaaagcg
    26281 gttctttctt tgcgcaatct ttggcgtggc tacaaatcag ctggctttct ttaaaggctt
    26341 gtcgatgacc agcccgatca gcgcttccat tattatgact atcaatcctg taatggtgct
    26401 gattttttcc tggttaattt tgaaagaaag agtaaccgcg ttaaaggttt tggggatttt
    26461 actgggagca gcaggcgccg ctttaatgat aagtcgtaac ggaattgatt tcaac
//
LOCUS       DASWHX010000119         5694 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_3073274, whole genome shotgun sequence.
ACCESSION   DASWHX010000119 DASWHX010000000
VERSION     DASWHX010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 5694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5694
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3073274"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            413..1231
                     /locus_tag="VGA21_08305"
     CDS             413..1231
                     /locus_tag="VGA21_08305"
                     /inference="COORDINATES: protein motif:HMM:NF042696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX9650548.1"
                     /translation="MITKFHIPAYPLNQFIDHFFYYVGYNPEHSVDRFLPDGEVQIIF
                     DLTDYPKFIYDNETLKEIQSCQNVWFSGFRTEPITIPSGKESEMLIVQFKKGKAFPFL
                     NEPMYNLTNFVVDAELVLKSDILNIRESILESPTPIKKFQVLEKRLLACYINQLNESP
                     LIDFAISKILSSPNQCSIKEISDNAGFSQKHIIKLFKEKVGVTPKEFLKVIRFQKAIQ
                     QIDHQISADWPAIAYDCGFYDQSHFIADFKLFSGFTPTEYARQKGDFLNYIPIK"
     gene            1334..1741
                     /locus_tag="VGA21_08310"
     CDS             1334..1741
                     /locus_tag="VGA21_08310"
                     /inference="COORDINATES: protein motif:HMM:NF020157.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1761 domain-containing protein"
                     /protein_id="HEX9650549.1"
                     /translation="MENMFINHWAVFACVIFNLVLGAIWYSPALFYNAWKKENNLSDE
                     QLKQSNPAKTYGITFLLAYLMSYNLAFFLGDANTDWQWGTTAGFLAGFGWAAAIFAVI
                     ALFEQKSWKYIFINGGYIVIYFTVIGFILGIWR"
     gene            1753..2325
                     /locus_tag="VGA21_08315"
     CDS             1753..2325
                     /locus_tag="VGA21_08315"
                     /inference="COORDINATES: protein motif:HMM:NF039496.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5655 domain-containing protein"
                     /protein_id="HEX9650550.1"
                     /translation="MAKTSQEIEKEFIDGLKPGTGNDIKDWLSTIKESEIEKRNDIIK
                     WLKGQHKFGHMNASLLAGIYFNGGKPVYASEKNLLDSQFEKYQDMRPLFEELQRTISK
                     RDASVNFVIKKTYVSITKKREFAAINIKKGELRLGLDLGEMPFKDFVEKAKLTGPMPR
                     ISHMVVIRNRSEISNQLFQLLDRADKRVNG"
     gene            3023..5179
                     /locus_tag="VGA21_08320"
     CDS             3023..5179
                     /locus_tag="VGA21_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017730704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX9650551.1"
                     /translation="MTDYFKHLMHEFELPLGNPVLIFSLILFIILLSPILLRRLNIPS
                     IIGLIISGVIIGPYGLNILEKNSAVDLFSTIGLLYIMFIAGLELDMNEFKSNRNKSLV
                     FGFFTFFFPLAIGFPVCYYFLEYDFNASFLTASMFATHTLVAYPIVSKLGVSKNQAVA
                     ITVGGTILTDTAVLIILAVIIGNSQGNLNMEFWIRLGISLAIFSAIMFLIIPRIAEWF
                     FRKLESEKHTHYIFVLSMVFLAAFLAEVAGFEPIIGAFVAGLALNKLIPHSSALMNRI
                     EFIGNSLFIPFFLISVGMLVDVNVIIGGPTALIVVGTLSVVALFGKWFAALFTQLVFK
                     YSKAQRQLIFGLSSAHAAATLAVILVGYKAEILDENILNGTIILILITCIVASFATEK
                     AAKKIVIETEDDTTGLKANGVHNEHILLPIANVENIEKLLEFSIFIKDKKSANPVSVL
                     SVVSNNDEAEINILKARIKLEEFVKQASASETKVNIITTIDHNAASGIARISREIIAD
                     IIVLGWPKRTGFIDKLIGEKVDNILSNTDKTTFICHLEKPLVLHKRIVIAAPPLAEHE
                     NGFELWFSKIAKLAQELSIPIQLFCNELTEKSIGRAVKKSKLSASITVSHFSDWQDFL
                     ILARNIHEDDLFVLASARKGATSYMAVLENLPTKLEKHFPSNSRLVIYPQQFIQNYGS
                     ERYDDINAEPLNKGIETMKKIRKGIGNIFKKKGRNE"
BASE COUNT         1841 a         1082 c         1070 g         1701 t
ORIGIN      
        1 tcggcctgtt gcttttgaat tttagcatct tcttgaggtt gtaggcaatg gctgtgccac
       61 acttccactt tttattttag gtgaaaattg attgattttc aatgtcagca aagtgtggca
      121 cacctggaag gcctcaaata cggattcatc aataaagaat gtatttacta tgctactttc
      181 acgagtgaat gttggataga ttaatttaca tgaaaaaata atccggaaaa ttatgtgttc
      241 agcccggcaa ttaattatat taatgaaaag gacgggatgc tggcacttga tggctttgtg
      301 gaatgaaagc attacaactg caaagttcat cggcatcgct caatcgcgag ccaacctttg
      361 ttttaatacg ccgcctgctc ttagcctttt gtaatgaaaa acatattaaa acttgattac
      421 aaaatttcac atacccgctt acccccttaa tcaatttatt gaccactttt tctattatgt
      481 gggttacaat cctgaacatt cggttgacag atttttacct gacggagaag ttcaaataat
      541 tttcgacctt accgattatc caaagttcat ttacgacaat gagacactaa aagaaattca
      601 atcctgccaa aatgtttggt tttcaggttt taggacagaa cctataacaa ttccttcagg
      661 taaggaaagt gaaatgctga ttgttcagtt taaaaaaggt aaggctttcc catttctgaa
      721 tgaaccaatg tataacctga caaattttgt ggtggacgct gaactagttt taaaatcgga
      781 tattctaaac atccgggaaa gtattttgga gtcaccaaca cctataaaaa aatttcaggt
      841 tctggaaaaa cgattgttgg cttgttatat caaccaatta aacgaaagcc ctcttattga
      901 ttttgcaata tctaaaatac tgagttctcc aaatcaatgt agtatcaaag aaatctcgga
      961 caatgcaggc ttttcccaaa agcatatcat caaactgttt aaagaaaaag ttggggttac
     1021 acctaaggag tttttaaaag ttattcggtt tcaaaaagcc attcaacaaa ttgatcacca
     1081 aatttccgct gactggcctg ctattgccta tgattgcgga ttttatgacc aatcccattt
     1141 tattgcggac ttcaaattgt tctctggctt cactccaacc gaatatgcac gtcaaaaggg
     1201 cgatttccta aattacattc ctataaagta gaacaagccc gcctgccgta atgtaatgaa
     1261 ggcaggtaaa atttttccta tcacagataa atccgattat ggaattttgt cctgaactta
     1321 aaaaagaaat cttatggaaa atatgtttat caaccattgg gctgtatttg cctgtgtaat
     1381 tttcaacctg gtattggggg ctatctggta ttcacccgcc ctgttttaca atgcctggaa
     1441 aaaggagaat aacctatcag acgaacaact aaagcaatcg aacccggcca agacttacgg
     1501 cattactttt ttgctggctt acttaatgtc ctacaacctc gcatttttct taggagacgc
     1561 aaatacggat tggcaatggg gaacgacagc aggatttctt gcggggtttg gatgggcggc
     1621 agccattttt gcagtaatcg ccttgtttga gcagaaaagc tggaaataca ttttcatcaa
     1681 cggcggatat atagttattt attttacggt aattggtttc attctgggaa tctggagata
     1741 aaaacaaaag tcatggcaaa aacttcacag gaaatagaaa aagaatttat tgatggacta
     1801 aaacctggca cggggaatga cataaaggat tggctgagca ccatcaaaga aagtgaaatt
     1861 gaaaagcgaa acgacataat caaatggcta aaaggacagc ataaatttgg acacatgaac
     1921 gccagccttt tggcgggtat ttatttcaac ggtggtaagc ccgtatatgc cagcgagaaa
     1981 aatttactgg atagccagtt tgaaaaatat caggatatgc gaccgctttt tgaggaactc
     2041 caaaggacaa tctcgaaaag ggatgcttcg gttaattttg taattaagaa aacgtatgtg
     2101 tctattacaa aaaaacgaga gtttgccgcc attaacatca aaaaaggcga gctgcgttta
     2161 ggattggatt taggtgaaat gccatttaaa gattttgttg aaaaagcaaa attgaccggc
     2221 ccaatgccaa gaatttcgca catggttgtc attcgcaatc gctcagaaat aagtaaccag
     2281 ctatttcaat tgctggatag ggcagacaaa cgagtaaacg gttgaagaca atatatataa
     2341 agcatcaaga ttaaatatga attaataaaa acaagtaagc taatcaagta aaaacaggaa
     2401 gtctcatccc cttccttttc tgaacccaca ggtgtaagca cattcgctca gcattgtata
     2461 aaagcaatac aggttgagtt tgaacagaag gatcaataca atttaataac ttaatcgcgg
     2521 tttgaaagag tagtactttt aataccactt ttatacataa ccgttatgcc caattgtaaa
     2581 acgacaccaa acgattgaca ttcaaacttt caacaaatca aagtcatacc catacatgtt
     2641 gtcctggccc cctattctct gcacagaatc agattcagtc gataagcagc gcaaagaggt
     2701 gtttgtgcaa gggttttcaa ggccgactga ttccttaaaa aatcaatcac aaaatcagtc
     2761 gctcgctacc agccttgaca agcctgaaat aaacacctac ttttgctcgt cggctgaatc
     2821 ggacaagcac aattactaca ctactttaaa ccggattctg tgcagagaat agggggctaa
     2881 gacaacattt ggcacatccc acttatccat tgcacaaagc cgaaccacta aaaagcgaga
     2941 agagccaaat ttttccagcc ctaaaaaagg aattgagtat attcataacg agaattatta
     3001 tagaaaagga taaaacaaaa agatgacaga ttattttaag cacttaatgc acgaatttga
     3061 attaccactt ggaaatccag tactcatatt ttcattaatc ctgtttatca ttctgttatc
     3121 tcccattctg ctgagaagat taaatattcc cagtataatc ggactaatta tttctggcgt
     3181 tattatcggc ccgtatggac tcaatattct cgaaaaaaat tctgcggttg acctcttttc
     3241 aacgattgga ctgctgtaca ttatgttcat agctggtctc gaattggata tgaacgagtt
     3301 taaatcgaat cgaaataaaa gtctcgtatt tggatttttc acattctttt tccctttagc
     3361 aataggtttt cccgtttgtt attactttct cgaatatgac tttaatgcca gttttcttac
     3421 tgccagcatg tttgccacac acacactcgt ggcatatcca attgtaagca aattgggagt
     3481 ttcaaaaaat caggcagtcg ccatcacagt tggcggaacc atattaacag atacagccgt
     3541 acttatcatt ctggctgtaa ttattggaaa cagtcagggt aatttgaata tggaattttg
     3601 gattcgattg ggcatttcat tagctatttt ttctgctatt atgttcctta taattccaag
     3661 aattgccgaa tggttctttc gtaaacttga aagtgaaaaa cacactcatt atattttcgt
     3721 cctgtccatg gtgtttttgg ctgcattttt ggcagaagta gcaggctttg agccaatcat
     3781 tggagcattc gtagcaggat tagccttaaa caaacttatc ccacattctt cagcattgat
     3841 gaatcgaatt gaatttatcg gcaactcatt attcattcct tttttcttga taagcgtagg
     3901 aatgttagtt gatgtaaacg ttattatcgg tggccccacg gcacttattg tagtaggaac
     3961 gctttcagta gttgcgttat ttggaaaatg gtttgctgca ttatttacac agctcgtttt
     4021 taaatactct aaagcacaac gacagctcat tttcggtctg agcagtgcgc atgcggctgc
     4081 aacgcttgcg gtaatcttgg taggttataa agctgaaatt ctggacgaaa atattctgaa
     4141 cggaactatt atactaattc taatcacttg tattgtggct tcttttgcca cagagaaagc
     4201 tgctaaaaaa atcgtcattg aaactgaaga tgatacaaca ggcttaaaag caaatggagt
     4261 tcataatgaa cacattttat tgcccattgc aaatgttgag aatattgaaa aactacttga
     4321 attttcaata tttattaagg acaaaaaatc agccaacccc gtatcagtcc tttctgtagt
     4381 ttccaataat gatgaagcag aaataaatat tttaaaagca agaatcaaac tcgaagaatt
     4441 cgtaaaacaa gcttctgcat cagaaacgaa agtaaatatt atcaccacca ttgatcacaa
     4501 cgcagcaagt ggaatagctc gtatttcaag agaaataata gctgacatca ttgttttggg
     4561 gtggccaaaa cgcaccggat ttattgacaa gttgattggc gaaaaagtgg ataatatttt
     4621 aagcaacacc gacaaaacaa cttttatctg tcatcttgaa aaacccttgg tattacataa
     4681 aagaattgta attgcagctc cacctttagc ggaacatgaa aacgggtttg aactttggtt
     4741 ttctaaaatt gcaaaattgg ctcaggaatt aagcataccc attcagttat tttgcaatga
     4801 attaacagag aaatccattg gaagagcggt taaaaaatcg aaattatccg cttcaattac
     4861 cgtgagtcat tttagtgact ggcaagactt cctgattctc gcaagaaaca tacatgaaga
     4921 tgatttattt gtgttggctt ctgctcgtaa gggggctact tcatatatgg ctgtgctcga
     4981 aaatcttccg actaaactcg aaaaacattt tccatccaat agccgacttg taatttatcc
     5041 gcagcagttc attcagaatt atgggagtga acgatatgat gatattaatg cggaaccgtt
     5101 gaataaagga attgaaacca tgaaaaaaat taggaagggc attggaaaca tctttaaaaa
     5161 aaaaggccgc aatgaataat ctgcttgaag aacattgata ccaccgaagg ctacttccct
     5221 gacttttggt tgccgcaatt tggagtgcct ttttttgaca tcacccgtta cagctttgtt
     5281 ctactttgaa catagggttg gcaaactcga agtttgaacc tccctatcga ttttctaaca
     5341 acttaacctt ctgttgcatc aggtcagtac cggagtttgt agtctcgctt cctgcagttc
     5401 ttgcttcgca gctaacaggc ttcaccaact tgaccgcatg ggacttactc cctctggaat
     5461 aagtcggcat ctttcaatac ctttgcccaa tgccttgagc gcacactggc ttaaacgaaa
     5521 tggcaggtga agtgcttaat caaacattag tggatttaat taactttggt gcagggcgaa
     5581 agtatggtgc atctatttgc cgtcacttcg ttaagtcccg aaccatacgg caacatttaa
     5641 aatcaaaaga aacgaagact caatgaaaga atcaatcacg ataattttct tatt
//
LOCUS       DASWHX010000120        28491 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_3076995, whole genome shotgun sequence.
ACCESSION   DASWHX010000120 DASWHX010000000
VERSION     DASWHX010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 28491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28491
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3076995"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1512
                     /gene="dxs"
                     /locus_tag="VGA21_08325"
     CDS             <1..1512
                     /gene="dxs"
                     /locus_tag="VGA21_08325"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013453598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEX9650552.1"
                     /translation="NKYKHDPRHHIAVIGDGALTGGIAFEAMNHAGVSNTDLLIILND
                     NCMSIDPNVGALKDYLTDITTSPTYNKVKDEVWNLLGKISKFGKNAQEIASKFDTSVK
                     SFLLQQSNLFESLNLRYFGPIDGHDVSHLVSILQDLKEIKGPKLLHCVTVKGKGFPEA
                     EKDQTKWHAPGKFDKITGEIFKVVDAKPKAPKYQDVFGHTLVELAEANEKIIGVTPAM
                     PSGSSMNIMMNAMPDRAFDVGIAEQHAVTFSAGLATQGLIPFCNIYSTFMQRAYDQVI
                     HDTCIQNLPVNFCLDRAGFAGADGPTHHGAYDIAYMRCVPNMIVSAPMNEQELRNLMY
                     TAQLPRAGKAFTIRYPRGEGVMPNWKTAMEGIEIGTGRILYEGSGLAILTLGHIGNYA
                     TEVVERLKKEGINIGHYDLRFAKPLDDKLLHRIFKNYTKIITVEDGCIAGGFGSAILE
                     FMNENGYHAIVKRLGIPDEIIEHGTQLELHRECGFDPGGIYACALALLEASTI"
     gene            1649..2407
                     /locus_tag="VGA21_08330"
     CDS             1649..2407
                     /locus_tag="VGA21_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009579223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650553.1"
                     /translation="MEEAPLPFLDTWNQWAQIVAGLLAALAVITLLTHLFKLATTGDP
                     KTKYDFINKSEISVLWTFSLMLVFGAAIFANSFLAGVGLMWLIIRLFVSLVLSVIILV
                     VIRNLLKFYYPFYMEKRLKALRYRPRISPKTGKPMKLLSEEEEDVYLDEGMQAEEEIF
                     SIDYDVWIDEESGYTQIEKYSGHLHALKCPECDYQTFKIVKEEILRNPTPTVEGELTK
                     FFECGYCGFKEARNVRVAPMKDPASRSKSTAAPV"
     gene            complement(2411..3853)
                     /locus_tag="VGA21_08335"
     CDS             complement(2411..3853)
                     /locus_tag="VGA21_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009580574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HEX9650554.1"
                     /translation="MEKLQNYINGVLTDPVSGEFIENYDPSIGKPYSLIPDSDERDVE
                     AATIAARNTYDQWSATPTKERSDILLKIADLIDNNLDALARMESIDNGKPLKLAKRVD
                     IPRASANFRFYATAALHFSSEAHLTDRDAINYTLRSPIGVAGCISPWNLPLYLFTWKI
                     APALATGNTVVSKPSELTPMTAFKLSEICMEAGLPAGALNIVHGLGPKAGQAIIEHSD
                     IPLISFTGGTATGKIIASTAGPMFKKLSLELGGKNPNIIFEDCDFEQAVSTSLHSSFS
                     NQGEICLSGSRIFIQKKIYEKFKNAFLNKVKEFTVGDPLEEETKVGALVSENHMKKVL
                     SYIELARQEGGTVLSGGDRVTLQGRCRNGYFIEPTVIEGLPHDCRTNQEEIFGPVVTL
                     MPFDTEEEVIKYANSTKYGLSATIWTENLKRAHRIAAQIKSGIIWVNCWLLRDLRTPF
                     GGMKQSGVGREGGLEALRFFTEPKNICIKL"
     gene            complement(3858..4646)
                     /locus_tag="VGA21_08340"
     CDS             complement(3858..4646)
                     /locus_tag="VGA21_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018476513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9650555.1"
                     /translation="MNLSLEGKTAIVCGSTQGIGKASALELASLGANIVLMARNEERL
                     TKVTNELEKSPGQQHACLVADFSKPAEVREAIVGFVTKNPSVHILVNNTGGPPAGTAI
                     DAAENDFISAFQSHLICNQILVKAVVPLMKKEAYGRIINIISTSVKTPIAGLGVSNTI
                     RGAVANWSKTLAYELGGFGITVNNVLPGLTKTGRLEEIIEGRAIKFEKTEEEIEREWL
                     EQIPARRFADPEEVAWAVAFLATPAAAYINGVNLPVDGGRTPSL"
     gene            4771..5295
                     /locus_tag="VGA21_08345"
     CDS             4771..5295
                     /locus_tag="VGA21_08345"
                     /EC_number="1.13.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017730651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000334 - 3-hydroxyanthranilate
                     3,4-dioxygenase activity [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyanthranilate 3,4-dioxygenase"
                     /protein_id="HEX9650556.1"
                     /translation="MTIQKPFNFKGWIEENRHLLKPPVGNQQVYKGNKDYIVMVVGGP
                     NSRKDFHYNETEEFFYQVEGDINLRIMEDGKPVDIPIKEGDIFLLPPGVPHSPQRPAN
                     TVGLVMELYRKHNEKDGFLWFCEKCNEKLYEEYFELVDIVKQLPPIMQKFYDSQELRT
                     CKNCGTEMEPPIKK"
     gene            5292..6326
                     /locus_tag="VGA21_08350"
     CDS             5292..6326
                     /locus_tag="VGA21_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018476516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX9650557.1"
                     /translation="MRDHSGSFSIDIHTHILPKTWPDLRERYGYAGFIRLEHHKPCAA
                     RMMMDDKFFREVQDNCWDSEVRKKECDQLHVDLQVLSTVPVMFSYWAKPEHTHDLSRI
                     LNDHIASVIHKYPQRFAGLGTLPMQDSDLAISELERCKDLGLLGVQIGTHVNDLNLDA
                     PAIFSVLEAAQELNQCIFIHPWDMMGSKEMPKYWLPWLVGMPAETSRAIVSMIFGGVF
                     ERLPKLRVAFAHGGGSFPATIGRIEHGFTVRPDLCAVDNNVNPREYLNKFYLDSLVHD
                     KTMLKYIVSLMGDENIILGSDYPFPLGEAEPGKLINEAGFSKRTREKLLSGNALRWLG
                     VDKDYFRKKG"
     gene            6372..7643
                     /gene="kynU"
                     /locus_tag="VGA21_08355"
     CDS             6372..7643
                     /gene="kynU"
                     /locus_tag="VGA21_08355"
                     /EC_number="3.7.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014796029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030429 - kynureninase activity [Evidence
                     IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kynureninase"
                     /protein_id="HEX9650558.1"
                     /translation="MHYTSEKNFAEEADRKDLLKNFKDLFYFPVRNGKQQYYFTGNSL
                     GLQPKNVRSFVELELKAWEEYAVEAHFEGKNPWFHYHKFTKKHLAELTGAKELEVVSM
                     NNLTTNLHLMLTSFYQPKGRKNKIIIEAGAFPSDQYALETQIKLRGLIPDEILIELQP
                     EPGEHCLRTDNILSAIRELNEELALVMMSGVQYYSGQFFEIDKITQAAHEVNAYAGFD
                     LAHAIGNLPLSLHKDDVDFAVWCSYKYLNSGPGGVSGIYVNEKHGSNNTIPRLGGWWG
                     QNEEIRFKMQKGFHPMKGADGWQLSNVNVISTAAHRASLEIFSKAGINTIREKSKILT
                     GYLEYLLNDTMSGNYFEIITPSNPEERGAQLSLLFKKNGKEVFEALTKDNMLVDWREP
                     NVVRISPVPLYNSFTDVFALGNSLRNILGQL"
     gene            7640..8986
                     /locus_tag="VGA21_08360"
     CDS             7640..8986
                     /locus_tag="VGA21_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX9650559.1"
                     /translation="MSTRKRILIVGAGPVGSLLAIYLIRKNYAVTLLERRPDMRKSEL
                     DAGRSINLALSNRGLRPLIELGLQKRVSKMIIPMKGRFIHDASGKTSMQPYGVEGQVI
                     NSISRPGLNELLLNVAENEGCRIIFDSRATEIDVEATKVESIERGKKVIYDGDVIIGS
                     DGAFSAIRTSIMKTDRFDYSQFYIEHGYKELSIPSNSDGSFRLEKNALHIWPRGHFML
                     IALPNLDGSFTVTLFFPFEGEPSFASLNTKKKVRDFFKATFVDAFPHLVDLDKEFEEN
                     PTSSLVTVRCFPWVKNNTLLIGDAAHAVVPFYGQGMNCGFEDCRVLNSILTGNNEDWR
                     KTLSEFEKIRKPDADAISELALRNFIEMRDLVANENFVLQKKIESKLHKMFPERWIPL
                     YSMVTFQDHIRYSDALKIGNKQQEIMNDLLKKEDIKDTWESLDFASIVKRLESQKI"
     gene            complement(8983..9429)
                     /locus_tag="VGA21_08365"
     CDS             complement(8983..9429)
                     /locus_tag="VGA21_08365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650560.1"
                     /translation="MAKVDELFSEYSSSRAHDLNVDQFTAILKLVPGLIIATSDGVMD
                     SRELGLVNKFAGMMGDELIPDNVEGVVEKEEKLMKDIRTELQFIMKNISAWREKILDA
                     LAENIKNDKKQKEFVGEVMHLFASTSGGYSTEEEKKIDEMYTRLSL"
     gene            complement(9480..10952)
                     /locus_tag="VGA21_08370"
     CDS             complement(9480..10952)
                     /locus_tag="VGA21_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017730656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEX9650561.1"
                     /translation="MVSDKVNAIISLLAMACVSLLTGCGKSITESNSDSTSVARDPIP
                     LPVDFEFDKIIERDTLIAIIDNSSTSFFVYKGQPMGFEYELLKLLSDDFGVNLKLIQT
                     TSIDKAFRMLNTGVGDIIAYSLTVTSERKKIVKFTRNQYTTRQVLIQRKPENWPQMKL
                     HEIEAQLIRNQVDLIGKQVHVRNSSSFLYRLIHLSEEIGGEILIVEESEEADTEALIR
                     RVAVGEIDYTIADEDVALINAAYYPILDVKTPVSFPQRIAWAIRKNSSQLEEQINNWL
                     MRLQREPTYNLLYEKYFENPRVALIRATSDYSTIHGGSISEYDALIKQWADSLKWDWK
                     LLASQIFQESRFDPRAVSWAGAKGLMQLMDNTARQYNVRNQFDPRQSIRGGVRYLIYL
                     DKFWSDYISDPNEKLKFILASYNVGLGHVIDARKLAQKYGRDPSIWDDNVAYYLLHKS
                     DPRFYKDPVVESGYCKGEEPVLYVTEILYRYGIYQQLVSS"
     gene            complement(11021..11875)
                     /locus_tag="VGA21_08375"
     CDS             complement(11021..11875)
                     /locus_tag="VGA21_08375"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX9650562.1"
                     /translation="MFLLRLLSYLPFGALYFVSDILSFLTFYIFRYRRKVVFQNLRNS
                     FPEMSEKEIRKIAKRFYHNLADIIVEILKEITITKEEIIKRVKYVNPEELNPYFKDEK
                     SVIVVTIHQGNWEWLLLAAGVYHNKPADVLYSPLSNRYFDKIMIRTRSRFGGFPIPVS
                     NITREIVRRSKIPRMIVLLADQTPTPNSDIYWSSFLNQETAFYKGPDVLAKFTKYPVF
                     YAKITRIKRGYYQVKALKLGEPPYDDKTDILNKFIHESEKTIRENPSDWLWSHRRWKR
                     KRHSDLSK"
     gene            complement(11878..13773)
                     /gene="acs"
                     /locus_tag="VGA21_08380"
     CDS             complement(11878..13773)
                     /gene="acs"
                     /locus_tag="VGA21_08380"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019986855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HEX9650563.1"
                     /translation="MFTQINSHQEYLEIHKRSIENPEEFWSGIATDFLWKRKWNKVLK
                     WDFKKAEVRWFSGGKLNITENCLDRHLKNRGDQVAIIWEPNNPSEDTKEITYAQLHRE
                     ACKFANVLKENGIQKGDRICIYMPMVPELAVAMLGCARIGAVHSIVFAGFSSTSLSDR
                     INDAACKMVITSDGSFRGNKTVPLKEISDEALSKCKTVERVIVLKRTGQQINWFQNRD
                     IWWHEAMQGKSDTFDAVETDSEDLLFILYTSGSTGKPKGMVHTVAGYMVYTTYTFLTA
                     FQYKEKEIFWCTADIGWITGHSYIVYGPLSAGATSLMFEGIPSWPDMGRFWDVVDKHK
                     VNIFYTAPTAIRALEKADLRFVQNKNLSSLRVLGTVGEPINEEAWYWYDNHVGKNKCP
                     IVDTYWQTETGGFMISPIAGATKLKPAFASFPMPGIQPAIMDDKGNELHGNNIEGRLA
                     IKFPWPSIARTIYGDHERFRQTYFSTFKNMYFTGDGVKKDDEGYYRITGRVDDIIIVS
                     GHNLGTAEIENAIDEHAEVCETAVVGYPHDIKGQGIYAYVILYDHEHDHGRLRKEILD
                     TVSKTIGPIAKPDKIQFVSGLPKTRSGKIMRRILRKIASGETANLGDVSTLLDPSVVE
                     EIKQGAV"
     gene            complement(13814..14554)
                     /locus_tag="VGA21_08385"
     CDS             complement(13814..14554)
                     /locus_tag="VGA21_08385"
                     /inference="COORDINATES: protein motif:HMM:NF014582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HEX9650564.1"
                     /translation="MIVNLIILLLAGLSGVVLLYSPVNWKQENFRLTISFAGGFLLSI
                     SLLHILPDLFQSQLGAFKAGLFVLAGFFLLQILEFITDGAEHGHFHKHQIGQKHGFSF
                     SIFLLLGLSIHSFLEGSILAFPENAQVAISVRNLLIGIVLHKIPASMALSSVLICSVE
                     NKTVALGYLFIFALASPAGLILSHVFELASGASDFFLILFAIVCGNFLHISTTIVFES
                     SPEHKFNFQRLLWSLLGAGAAIIAELLN"
     gene            complement(14566..15594)
                     /locus_tag="VGA21_08390"
     CDS             complement(14566..15594)
                     /locus_tag="VGA21_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009184161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEX9650565.1"
                     /translation="MKRLFHKSRLYPLVLILVVFVVWKYRESKKPQLILLQGTTMGPI
                     KYTVKYLSSSGENFQPQIDSLLETFNQSLNHYRSGSEVSKFNTDSVFVFVSPYFLPVL
                     QKSREVFEITNGAFDPTVGPLVNAWGFGPGMKITLDSAMVDSLRQLVYFQNIEFDKTK
                     VWKKKKNIQLDFSAIAKGQGVDVVFEFLISRGLENIFVEIGGEVRCAGKNENGEYWKI
                     GIIDPANDPAQEPLYGTVSLENKSMATSGNYYNFIDEGGQRIVHTINPATGYSITSNL
                     LSATVIASDCMTADAFATAFMVLGLEKSISIVENNSELETILIFSNPDGTLAHYHSKG
                     VKGIFVKF"
     gene            15722..16753
                     /gene="ruvB"
                     /locus_tag="VGA21_08395"
     CDS             15722..16753
                     /gene="ruvB"
                     /locus_tag="VGA21_08395"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009580069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEX9650566.1"
                     /translation="MREDYLSAGDENLNPADKELEKALRPLSFRDFTGQFKVVENIQV
                     FVQAARKRGEALDHVLLHGPPGLGKTTLSHIIANELGTNIRITSGPILDKPGDLAGLL
                     TNLENNDVLFIDEIHRLNPVVEEYLYAAMEDFRIDIMLDSGPSARSIQITLNPFTLIG
                     ATTRIGLLTSPLRSRFGITSRLEYYDADLLNIIVRRSSAILEVEIDNDAAKEIAARSR
                     GTPRIANMLLRRTRDFAQIKGDGRITKEIADMALSALDVDKNGLDDMDNRILSTIIRK
                     FKGGPVGISTIATACGEEAETIEEVYEPFLIMEGYIKRTARGREATEKAYKHLNVLLP
                     GKLPGLFDA"
     gene            16710..17825
                     /gene="corA"
                     /locus_tag="VGA21_08400"
     CDS             16710..17825
                     /gene="corA"
                     /locus_tag="VGA21_08400"
                     /inference="COORDINATES: protein motif:HMM:NF013694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HEX9650567.1"
                     /translation="MYYCLENYLACLTHRIMFQFNIFRKQSRGLAPGSRIFVGDQKMD
                     VPRTTVMDFNPEDIQEKEIVDVAGLGEYKTTNNFTWINVDGVHDPILIEKIGNVFDID
                     SLVQEDIMNTTHRPKMEEHEDYLFIVMPMVWMDEKSKKENSEQLSLIIGQNYLISFQE
                     KTGDFFEPLRERIRKRKGSRVRTLGIDYLAYAIMDIVIDNYLHLIDLLGDQIEDLEDD
                     VLDDVDNEIISKINGFKRDINYLRKRVRPVREFIILLSKSESDLLSHTTIPFMKDLHD
                     HIQNAYENVETYREMLTDYLTLYHTGASTKLNDILRILTIFSAIFIPLTFIAGVYGTN
                     FQYFPELQLRYAYPIFWAVLIMVTIGMFIFFKRKKWL"
     gene            17816..18751
                     /gene="queG"
                     /locus_tag="VGA21_08405"
     CDS             17816..18751
                     /gene="queG"
                     /locus_tag="VGA21_08405"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002696041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HEX9650568.1"
                     /translation="MALSRKERNSKIIKEKAALLGFDFCGISRAEKLNDEEKKLENWL
                     KQGMHGEMAYMENHFDKRLDPAKLVPGARSVVSFIYNYYPGKKLAAENNYKIAKYAYG
                     TDYHFVIKKKLKLMLGELRKEIGDFEGRIFVDSAPVMERQWAAKSGLGWIGKNSLLLN
                     RNMGSFFFLAVMISDLELIPDGLIKDYCGTCTRCMDACPTDAIPMPYVVDGSRCISYF
                     TIELKNEIPVDYKEKFENWIFGCDICQDVCPWNKFSNPNNENEFKPSKNLSNMNKNDW
                     EELSEDTFQKLFRKSAVKRTGFRGLTRNISFVAKE"
     gene            complement(18811..18996)
                     /locus_tag="VGA21_08410"
     CDS             complement(18811..18996)
                     /locus_tag="VGA21_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008627831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650569.1"
                     /translation="MLEYVKTILRKVSFDAKLFEKELRKAILTLVRSELVELKQWCYE
                     NFGKTHSIVLERCFVAI"
     gene            19139..20581
                     /locus_tag="VGA21_08415"
     CDS             19139..20581
                     /locus_tag="VGA21_08415"
                     /inference="COORDINATES: protein motif:HMM:NF024973.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HEX9650570.1"
                     /translation="MKFSKVFLHIIIAFFSASIAFAQNEKVTIEFGPEEIAYNDMFTI
                     TITVQNGRLRSYDNFPDMPGFIKRGTSSSSNTNIINGQVSTSQSITQNYVPEKEGNFS
                     VPGFKMKVNGNDHSHPGQKIKVGPAREKRDPFRDPFDDIFGRRQQQQPQDFVDIEDEA
                     FLALTTDKSEVYLGEGFNATLAFYVAESNRAPLQFHDLGRQLSEILKRLKPSHCWEEN
                     FNIENINGETVLLDNKRYTQYKIYQAEFYPLNLDEIKFSSVGLEMIKYRVARNPSFFG
                     RNRQEDYKTFFSKPKSVKVRDLPPHPLKDKVSVGRFRLEERINTNPVITGSSFTYEFN
                     IYGEGNISALNKPEIKLDDNFDFYSPNIEQNIIRRSGKVTGSKLFTYFGIPNEPGEYD
                     LSDYISLIYFDPNREIYDTLKSSVILTAIGESKKNQTISASDLGSFYDRLNTGDNTLR
                     RRNALADIRLFANVMILVLVVGGSALIFKK"
     gene            20592..21521
                     /locus_tag="VGA21_08420"
     CDS             20592..21521
                     /locus_tag="VGA21_08420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650571.1"
                     /translation="MKILISLHLAVLLIIWTNSVRTSELINEPESKILLVASTPADDA
                     VKAMLGIPIDLEVDFIRWEITFSQNQSPDRFILNLIYGKAQPNTLGFVAGGNKKTFTG
                     TYLTKVKSDGNLQGEIYELSEDDASRQISLVKLSENLFHLVTPDNKLFVGNGGWSYTF
                     NRNNNQSNIFPSEFLRLQSSIPRYSTNVITFDGRTPCYPFSIFKESQISGDCIKTKWS
                     LILHTDPETSLPTGYELKTTFNRTSTIQGKWSVTMGFGANPQAVIFQLNPDDPQKSVY
                     FLVGDENVLFFLDDEKQFLQGNSDFSFTLNKRE"
     gene            21660..22739
                     /gene="aroC"
                     /locus_tag="VGA21_08425"
     CDS             21660..22739
                     /gene="aroC"
                     /locus_tag="VGA21_08425"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010611875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEX9650572.1"
                     /translation="MFNSFGHVFRITTFGESHGPQIGVIIDGCPAGLKIDAQFIQSEL
                     NRRKPGQSKITSARKESDEFEIVSGVFEGMTTGMPVAILIPNKDAKSKDYSHLAKSYR
                     PSHADYTYAAKFGIRDYRGGGRSSARETALWVAAGAIAKLLLIKTKIDIRAYVSSVGN
                     IHASHYSKLNLKKTDSNIVRCPDKVAAKKMQAKIEAVKKSGDTLGGSITCVVKNTPVG
                     LGEPVFNKLHAALGGAMLSINAVKGFEYGSGFQGATMKGSEHNDIFINEKGVIKTKTN
                     HSGGIQGGISNGQDIYFKVAFKPVATIMQTQVSINEKKQRIKLQGKGRHDPCVVPRAV
                     PIVEALTALVLTDFYLINQTRKFSR"
     gene            22752..24044
                     /locus_tag="VGA21_08430"
     CDS             22752..24044
                     /locus_tag="VGA21_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015265347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HEX9650573.1"
                     /translation="MKKIPLYVKILIGMGLGVIWSIIAQNYELMDFTNDWIKPFGTIF
                     INGLKLIAVPLILVSLIDGISGLKDITKLSRIGGKTIAFYLFTTVLAITIGLLLVNTI
                     KPGKFLTPEKREELREAYASDAQLRVTEAQSEQEKGPLQMFVNIVPDNVFQSMTDNRA
                     MLQVIFFSFMFGIAMIFVGEEKVKNVKGFFSGVNVIILKIVDIIMRYSPIGVFALLGS
                     LSIDASMFKALGVYSLTVLAGLALMVVVLYPLIIRIFTHIKYRHFLKGIFPAQILAFS
                     TSSSAATLPVTMERVEKKLGVSEEVSSFVLPLGATVNMDGTAIHQAVSAVFIAQAFGH
                     DLQLADQLVIVLTATLSSIGAAAVPSAGLIMLVIVLSAIGVDPEGLALIIALDRPLDM
                     CRTVVNVTGDATVATLVASSEGELATGIDEARLNADDE"
     gene            24082..24672
                     /locus_tag="VGA21_08435"
     CDS             24082..24672
                     /locus_tag="VGA21_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009184272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HEX9650574.1"
                     /translation="MIEAKEKDPVSIYMESNPNPNSLKFVANKMLIQEGKSYDFPDIE
                     SAEFAPLARELFALDYVDRVFYMSNFVTVTKKENIEWVEIQNELKSKIKSFLESDKPL
                     LTKEPEPYQPEIETVADSDTVARIKEVLDEYVKPAVEMDGGAIQFESFGEGVLKVNLQ
                     GSCSGCPSSTVTLKAGIENLLKKMVPEVKEVQALGV"
     gene            24696..25967
                     /locus_tag="VGA21_08440"
     CDS             24696..25967
                     /locus_tag="VGA21_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019989090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HEX9650575.1"
                     /translation="MKKSAAEALACVQSGNHIFIHSVAAAPQHLIRALADRSSELRDV
                     QIYQLHTEGVAPYADPGMEKSFHTNCFFIGANVRKAVEEGRGSYIPVFLSEVPYLIRK
                     EIVRIDVSLITVSPPDKHGFCSLGTSVDATRSAIEMSEIVIAQVNHYMPRTHGDAQIH
                     IGKLNFIIEHDEPLVEHGPPVIDELTRKVGIHVASLIDDGATLQMGIGAIPNAVLGQL
                     KMHKDLGIHTEMFSDGIIPLVESGVITGKYKVKYPGRVVATFVMGTKKLYDFVDDNPL
                     MALLECSYVNDTKIIRQNPRVTAINSAIEIDLTGQVCADSIGSRMYSGVGGQMDFIRG
                     ASLSQGGKAIIALPSITSRGETRIVPQLKDGAGVVTTRAHVHYVVTENGIAHLYGKTL
                     KERAKELITISHPDHQEGLDRVFHERFKSLM"
     gene            26045..26119
                     /locus_tag="VGA21_08445"
     tRNA            26045..26119
                     /locus_tag="VGA21_08445"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26080..26082,aa:Val,seq:gac)
     gene            26352..26837
                     /locus_tag="VGA21_08450"
     CDS             26352..26837
                     /locus_tag="VGA21_08450"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HEX9650576.1"
                     /translation="MGLENIAYGRLFARIAEAYLNVLSRKVEPFGLDRYFGALLYIVE
                     NSGKITQKQLGRHLSKDKVSVLRCVNYLIAQGLVLKKMNQQDKRESFLAASEKSKKLA
                     PIISVAIRDTNNILFNNFQKKEFKEFETGLKKLLTVVDKMPDPDFIIRAEKKKNLVFE
                     R"
     gene            26834..27883
                     /locus_tag="VGA21_08455"
     CDS             26834..27883
                     /locus_tag="VGA21_08455"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX9650577.1"
                     /translation="MNKKLLLVTGMLGILFPGTSCQEGKQQAVPISRSLLQVEAIVAK
                     PRRFENKFTATANLLAFEEVELRAPVSGTVLGIYFQEAQNVKQGDLLVRLDDRTLNAQ
                     IKGLKVQLEASLSDLRRKKELLKVEGVSEEEVEQSQAVVDGIKSSMDELTVSVNLANI
                     RAPFDGRVGMRNFSMGSYLSQGQTITQLVQTQKLKVDFNLASRYLDEVKVGKKIKVIS
                     SSDTLEAEVYAISPFVNATSRTAQVRAIIQNNNRGFIPGDFAEVQVALNVDNEALVVP
                     TDVIIPDLNAQTLFIYRNGRAMRTEVLLGSRTDTEVQILSGLSPGDTVLTTGLLQVKD
                     LTQVEITEFVKPVTL"
     gene            27880..>28491
                     /locus_tag="VGA21_08460"
     CDS             27880..>28491
                     /locus_tag="VGA21_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020530312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX9650578.1"
                     /translation="MTLSELSIKRPVLATVFAITIVLFGFVGMYSLGVREYPSVDPPV
                     VTVSTSYTGANAGIIESQITEPLEEAINSVAGIKSLRSTSSDGRSSVTIEFETDVDIE
                     DAANDVRDKVSQAVRSLPPDANPPVVSKADADAQTIFSLTVQSDKRTLLELTELGNNV
                     FKERLQTIPGVSSIRVWGERKYAMRIILNPDKLEAFSLTPLDIR"
BASE COUNT         8633 a         5577 c         6109 g         8172 t
ORIGIN      
        1 aataagtata agcatgatcc ccggcatcac attgcggtta tcggggatgg ggcccttaca
       61 ggtggaatcg cctttgaagc gatgaatcat gcaggtgttt caaacacaga ccttcttatt
      121 attctcaacg acaactgcat gtctattgat ccaaatgttg gtgctctgaa agattacctt
      181 acggatataa caacatcccc tacttacaat aaagtaaaag acgaagtatg gaatttgtta
      241 gggaagatta gcaaattcgg aaagaatgcc caagaaatag cttcaaaatt tgataccagt
      301 gttaaatcct ttcttctgca acagagcaat ttgtttgaat cacttaacct cagatatttt
      361 ggtcccatcg atggccatga tgtgagccat ttagtaagta ttctgcagga tctgaaagag
      421 ataaaaggtc cgaaacttct tcattgtgtt accgtaaaag gaaaaggatt tccggaagca
      481 gagaaagatc aaaccaaatg gcacgctcct ggaaaatttg acaaaataac cggtgagatt
      541 tttaaggttg tcgatgcaaa accaaaagcg ccaaaatacc aggacgtgtt tggacatacc
      601 ctggtagaac ttgcggaggc gaatgaaaag ataattggcg ttacacccgc gatgccttcc
      661 ggctcttcaa tgaatataat gatgaatgct atgcctgatc gggccttcga tgtgggtata
      721 gcggagcaac acgcagttac tttttctgcc gggttggcta cgcagggcct gattccattt
      781 tgcaatattt acagcacttt catgcagcga gcctatgacc aggtgataca tgacacatgt
      841 attcaaaatc ttcctgttaa cttttgcctt gaccgcgccg ggtttgccgg agcagatggg
      901 cccacacacc atggagctta tgatatagcg tatatgcgct gcgttccgaa tatgattgta
      961 tccgctccaa tgaatgaaca ggagctccga aatttaatgt ataccgctca attgcccagg
     1021 gcaggtaaag cctttacaat ccgctatccg aggggtgaag gtgtaatgcc aaattggaag
     1081 actgccatgg aaggaattga aattggaacc ggaagaatac tctatgaagg ttccgggctt
     1141 gcaattctta ctcttggaca tattggaaat tacgccaccg aagtggttga aaggttgaag
     1201 aaagagggaa tcaatatagg tcattatgat cttcgtttcg ccaagccgct tgacgataaa
     1261 cttcttcaca ggatatttaa gaattatacc aaaataataa ctgttgaaga cggatgcata
     1321 gccggcgggt ttggaagcgc aattctggaa tttatgaatg aaaatggata ccatgctatc
     1381 gtgaaacgac ttggaatccc ggatgaaatc atagaacacg gcactcaatt ggaacttcac
     1441 cgcgaatgcg gatttgaccc cggtggaatc tacgcttgcg ccctggcttt attggaggct
     1501 agtacaattt aacagtcaaa cccttctatt cagcaaaaaa tccaaagagc tccctgtttc
     1561 ttataatttc aaatattttg ttttcataat attttcgtaa attgaattat taagagattt
     1621 tatcgccaat taaacctaat taatcaccat ggaagaagcg ccgttacctt ttcttgacac
     1681 ctggaaccaa tgggctcaga tcgtcgcggg gttgctcgct gcccttgccg taattacact
     1741 cctaactcat ttattcaaac ttgcaactac cggagaccct aaaacaaaat acgattttat
     1801 caacaagagt gaaatttcag tcttatggac gttttcatta atgctggttt ttggtgcagc
     1861 catttttgcg aattcattct tagcaggagt tggcctaatg tggctaataa tcagattatt
     1921 tgtaagtctg gttctaagtg tgattattct ggttgtgatc agaaatttgc tcaaatttta
     1981 ttatccattt tatatggaga agagactcaa agcacttcga tataggccca gaatttcacc
     2041 caaaacaggt aaacccatga agttgttgag tgaagaggaa gaagacgttt accttgacga
     2101 gggtatgcag gcggaggagg aaatattcag catcgattac gacgtatgga ttgatgagga
     2161 atccggatac actcagattg aaaagtattc cggtcacctt catgctctga aatgtcctga
     2221 atgcgactac cagactttca aaattgtaaa ggaagaaatt cttaggaatc cgacacctac
     2281 agtagaaggt gaactgacaa agttttttga atgcggatac tgcgggttta aggaagcaag
     2341 gaatgttagg gttgctccta tgaaagatcc ggcaagtcgg agtaaaagta ctgccgctcc
     2401 ggtataacga ttaaagtttt atacaaatgt ttttcggctc ggtaaaaaac cgaagagctt
     2461 ctagtccgcc ttctcttcct acacccgatt gtttcattcc cccaaatggc gttcttaagt
     2521 cgcggagcag ccagcagttg acccaaatta ttccgctctt gatttgcgcg gcaattctgt
     2581 gagctctctt caaattttct gtccatatag ttgcgctcaa accatacttg gtgctgttgg
     2641 cgtattttat aacttcttct tcagtgtcga aaggcatcag tgtaacaacg gggccaaaaa
     2701 tttcttcctg gttggttcgg cagtcgtgag gcaatccttc aataacagta ggttcgataa
     2761 aataaccatt gcggcatctt ccctgcaggg tcactctatc tccgccggat aatacagtgc
     2821 caccttcctg cctggccaat tcaatataag aaagaacttt cttcatatga ttttctgaca
     2881 ccaatgctcc aacttttgtt tcttcttcca gtggatcacc gaccgtaaat tctttcactt
     2941 tattcagaaa agcattctta aatttctcat aaattttctt ttgaatgaaa atcctggaac
     3001 cggaaaggca aatctccccc tggttggaaa aggaagaatg caaagaagta cttaccgctt
     3061 gttcaaaatc gcagtcttca aaaatgatgt ttggattttt accaccaagt tcaagggaca
     3121 atttcttgaa cataggccct gcagtggatg caatgatctt gccggtagct gttccaccgg
     3181 taaaggagat gagtgggatg tctgaatgtt ctatgattgc ctgtccggct tttggtccca
     3241 atccatgaac aatatttagc gctccggcag gtaaaccggc ttccatgcaa atttcagaaa
     3301 gtttgaacgc cgtcattggc gttaattcag aaggtttaga aaccaccgta tttccggtgg
     3361 ccagcgccgg agcaattttc caggtaaaca ggtacagggg cagattccag ggagagatgc
     3421 aacctgcaac accaatgggc gagcgcagcg tgtaattaat agcatccctg tctgtaagat
     3481 gagcttctga agaaaaatgc aaagcggcag ttgcatagaa tctgaaattt gcggaagccc
     3541 tcggtatatc tactcttttg gcaagcttta aaggcttccc attatcaatt gattccatcc
     3601 tggccagggc atccaggttg ttatcaatga gatcggcaat tttcaaaaga atatctgacc
     3661 gctctttagt tggagtggca gaccactgat cataggtatt tctggcagca atagtggcag
     3721 cctccacatc gcgttcatcc gaatccggaa tcaagctata aggtttgcca atagagggat
     3781 cgtaattttc aataaattct ccagaaaccg gatcagttaa aactccgtta atataattct
     3841 gtagtttttc cattctctta taaactgggt gttcggcctc cgtctacggg taaatttact
     3901 ccattaatgt atgccgctgc tggtgtggca agaaatgcca ctgcccaggc aacttcttca
     3961 gggtccgcaa aacggcgcgc gggaatttgc tccaaccact ccctttcaat ttcttcttcc
     4021 gttttttcaa acttaattgc tctgccttca ataatctcct caagcctgcc ggtttttgta
     4081 aggcctggca gaacattatt tactgttatg ccaaatcctc ccagctcata ggcaagagtt
     4141 ttcgaccagt tagcaacagc accccggatt gtattcgaaa cgccaagccc ggcaattgga
     4201 gtcttaactg aagtagagat aatattaata attctgccgt aagcctcctt cttcattaag
     4261 ggaactactg cttttactaa aatttgatta caaattaaat gactctgaaa cgcactgata
     4321 aagtcatttt cagccgcgtc aatagcagtc cctgcagggg ggccgccggt attattaaca
     4381 aggatatgaa cggaaggatt tttggtaaca aagcccacaa tagcttctct tacttcggca
     4441 ggtttagaaa aatcggcgac taagcatgcg tgttgttgac cgggagattt ttccagctcg
     4501 ttagtaactt tagtcagcct ttcttcgttt cgggccatta acacaatatt agctcccaaa
     4561 gacgccagtt caagggcgga tgcttttcct attccctgtg tggagccaca aactatggcc
     4621 gtcttacctt caaggcttag attcattatt aaataattgg tggaattgta aatataatca
     4681 aattgaggac agaccttttg aggatactct tctgaattag cggtttttta ttaattttat
     4741 tgaatagaaa caatataaaa attaacagaa atgacaatac aaaaaccttt caattttaaa
     4801 ggatggattg aagagaacag gcatctttta aagccgccgg tgggaaacca acaagtttat
     4861 aagggaaata aagactacat cgtaatggta gttggcggtc ccaattcaag gaaagatttt
     4921 cattataatg aaacagaaga atttttctac caggttgagg gtgacattaa tctaagaata
     4981 atggaagatg gaaagcccgt tgatataccc attaaagaag gagatatttt tcttcttcct
     5041 ccaggagttc cccattctcc acagagaccc gcaaataccg taggccttgt gatggaactt
     5101 taccgtaaac acaatgagaa agatggattc ctgtggtttt gcgaaaaatg caatgaaaaa
     5161 ctttacgagg aatattttga gcttgtggat attgtaaagc agctgccccc gattatgcaa
     5221 aaattctatg actcacaaga acttagaaca tgcaagaatt gcggtacgga aatggagcca
     5281 ccgattaaga aatgagagac cattccggtt cattttctat tgatatacat actcatatac
     5341 ttccgaaaac ctggcccgat ttaagagaaa gatacggata tgccggattc atccggcttg
     5401 agcatcataa accctgcgcg gccaggatga tgatggacga taaatttttt cgggaagttc
     5461 aggataactg ctgggattcg gaggtgagaa aaaaagaatg tgatcaactg cacgtagacc
     5521 ttcaggtatt gtcaacggtt ccggtaatgt tttcctattg ggcaaagccc gagcatactc
     5581 acgatttatc caggatactg aatgaccaca ttgcttctgt catccacaaa tatccgcaaa
     5641 gatttgccgg cttaggaact ttgccaatgc aggattctga cctggcaatt tcagaattgg
     5701 aacgctgcaa agatctggga ttgctcggag tacaaatagg cacccacgtt aatgatttaa
     5761 atctcgatgc cccggcaatc ttttcggtgc ttgaagctgc ccaggaatta aatcaatgca
     5821 tattcattca cccatgggac atgatgggtt ctaaggaaat gccaaaatac tggctgccat
     5881 ggctggtggg aatgccagcc gaaacctcca gggctatcgt gtcgatgatt tttggaggag
     5941 tatttgaacg attgccaaaa ttgcgggtgg catttgctca tggtggcggt tctttcccgg
     6001 caacgattgg cagaattgaa catggtttta ccgtgagacc tgatctttgt gcggtagaca
     6061 ataacgttaa cccccgggaa taccttaaca aattctacct ggattctctc gttcacgata
     6121 aaacaatgtt aaagtatatt gttagcctga tgggtgatga gaatataatc ctgggatctg
     6181 attatccatt tccattaggg gaggctgagc caggaaaatt gattaatgaa gcggggtttt
     6241 ccaagaggac acgcgaaaaa ttattatctg gaaacgcact tcggtggtta ggagtggata
     6301 aagactattt tcgaaaaaaa ggataaagga gatttcaaaa ttgaattaat aatctcaact
     6361 atttttggct aatgcattac acttcggaaa aaaattttgc tgaagaagct gatcgaaagg
     6421 atctgttaaa aaatttcaag gatttatttt acttcccggt gcggaatgga aaacaacaat
     6481 actatttcac cggcaattca cttggtttgc aacccaaaaa tgtccgaagt tttgtcgaac
     6541 tggagttaaa agcatgggaa gaatatgcgg tagaagcgca ttttgagggg aagaatccct
     6601 ggtttcatta ccacaagttt acaaagaagc atttggctga attgacaggg gcgaaggagt
     6661 tggaagttgt atcgatgaac aatttaacca ctaatcttca tttaatgctc acttcttttt
     6721 atcagccgaa gggcagaaag aataaaataa ttattgaagc aggcgcgttt ccgtctgatc
     6781 aatacgcttt agaaacgcag atcaaattaa gggggttgat tccggatgaa attctgatag
     6841 aattgcaacc tgaacccggc gaacactgtt tgcgtacgga caacattctt tcagcaataa
     6901 gagagctgaa tgaggaattg gctttagtaa tgatgagcgg agttcaatat tatagcgggc
     6961 aattctttga gattgataaa attactcagg cagcgcatga agtgaatgca tatgccggat
     7021 tcgaccttgc ccacgcgata ggaaacctac cattgagctt gcataaggat gatgtagatt
     7081 ttgccgtttg gtgcagctat aaatatctaa attcagggcc tggtggagtg tctggaattt
     7141 atgttaatga aaaacatggc agtaataata ctattcccag gttgggtggc tggtggggtc
     7201 agaatgaaga aataagattc aaaatgcaaa aagggtttca ccccatgaaa ggtgcggatg
     7261 gctggcagct aagcaatgtg aatgtaatca gcaccgcggc gcaccgggct tcccttgaaa
     7321 ttttttccaa agcgggaata aatactatca gagagaagag caaaattttg accggttacc
     7381 tggaatatct attgaatgat acaatgtcag gtaattattt tgaaataatt acaccctcaa
     7441 atcctgaaga acggggagcg caactttctc tactattcaa aaaaaatgga aaggaagtat
     7501 ttgaagcatt gacaaaagat aatatgctgg ttgactggcg ggaaccgaac gtagtacgca
     7561 tttcaccggt tcccttgtac aatagtttca cagatgtgtt cgccttgggt aactccctgc
     7621 gcaatattct cggccaatta tgagtaccag gaaaagaatc ttgattgttg gagccggacc
     7681 ggtaggttct ttgttggcaa tttaccttat cagaaagaat tatgcggtca ctttgcttga
     7741 aagaagaccg gatatgcgga aaagtgaact tgacgcgggc agatcaatta atcttgcttt
     7801 aagcaacagg ggcctgagac cactcattga attgggattg cagaagcggg tttcaaagat
     7861 gataattccg atgaagggca gatttattca tgatgcctca ggaaagacat ccatgcagcc
     7921 ctatggtgtg gagggtcagg taatcaattc catcagcagg cccggcttaa atgaattgct
     7981 gcttaatgtg gctgaaaatg agggatgtag aataatcttt gacagcaggg caacagaaat
     8041 agatgtcgaa gccacaaaag tggaatcaat tgaaagaggg aagaaagtaa tttatgatgg
     8101 cgatgtcatt atcggaagtg atggagcatt ttccgccatt cgcacatcaa taatgaaaac
     8161 cgacagattt gattactccc agttttatat agagcatggt tacaaggagt tgtcaattcc
     8221 ctccaattca gatggttcat tccgtctaga aaagaatgca ctgcatatct ggccacgtgg
     8281 gcattttatg cttatcgcct tacctaacct ggatggcagc tttacggtga cacttttttt
     8341 tccttttgaa ggagagccat cctttgcttc actgaataca aagaaaaaag tcagggattt
     8401 ctttaaggcg acatttgtgg atgcctttcc gcacctggtg gacctggata aagaatttga
     8461 agaaaatcct acttcgtcac tggttactgt gcgctgcttc ccctgggtca agaataatac
     8521 gctattaatc ggtgatgccg ctcatgcagt tgtacccttt tatggtcagg ggatgaattg
     8581 tggattcgaa gactgcaggg ttttaaattc tattcttacc gggaataacg aagattggcg
     8641 gaaaactctt tcggaatttg aaaaaattag aaaacccgat gcggacgcga tatctgaatt
     8701 agcattaaga aattttattg aaatgcggga cctggtagcc aatgaaaatt ttgtgcttca
     8761 aaagaagatc gaaagtaaat tgcacaaaat gtttcctgaa cgctggattc ccttatatag
     8821 catggttact tttcaggatc atatccggta ttcggatgcc ctcaagattg gaaataagca
     8881 gcaggaaata atgaatgacc ttcttaaaaa agaggacatt aaggatacat gggaatccct
     8941 ggattttgct tccattgtta aacggctgga aagtcagaaa atctaaaggg aaagcctggt
     9001 atacatttca tcaatctttt tttcttcctc agtggaatag ccgccggaag tagaggcaaa
     9061 taaatgcata acttctccaa caaattcttt ttgcttctta tcgttcttaa tgttttcagc
     9121 aagcgcatca agaatctttt ctcgccaggc tgaaatattt ttcatgataa actgaagctc
     9181 agttctgatg tctttcatca acttctcctc tttttccacg acaccttcga cattatccgg
     9241 aatcagttca tcacccatca tcccggcgaa tttgtttacc aggccgagct ctctggaatc
     9301 cattactcca tctgaggtgg ctataataag tccggggacc aattttagaa tcgccgtaaa
     9361 ctggtctaca ttaagatcgt gagctctgga agagctgtat tcggagaata attcatcaac
     9421 ttttgccatt gggatggtat atttaatcag gatgttaaat atattaaatg caaaccattt
     9481 tagctagaaa caagctgttg gtatatgccg tatctgtaaa gaatttccgt aacataaagt
     9541 acgggttcct cccctttaca atacccagat tcaacaacag ggtctttgta gaatctcggg
     9601 tcggatttgt gcaggaggta atacgcgaca ttatcgtccc aaatcgaggg atcccggcca
     9661 tatttttgtg ccagtttcct cgcgtcaatc acatgaccca gccccacatt atacgaagcc
     9721 agaatgaatt tcaatttctc gttggggtct gaaatataat cggaccagaa tttatcaagg
     9781 tatattaagt agcgtactcc gccccggata ctttgcctgg gatcaaattg gtttctaaca
     9841 ttatattgcc gggcggtatt atccattaat tgcatcagac ctttcgcccc ggcccaacta
     9901 actgcgcgtg gatcaaatct ggattcctgg aaaatctgag aagcaagcaa cttccaatcc
     9961 catttaagac tgtcagccca ttgcttaatc agggcatcat attctgagat actaccaccg
    10021 tgtatggttg agtaatcgct ggttgccctt atgagtgcaa ccctgggatt ttcaaaatat
    10081 ttttcataga gaagattata ggtaggctct ctctgaagtc tcataagcca attattaatt
    10141 tgttcttcaa gttgtgaaga attttttcga atcgcccagg caattctttg cggaaatgag
    10201 actggggttt taacatcaag aattggataa taagcagcat tgataagagc gacgtcttca
    10261 tcagcaatcg tgtaatcaat ttcaccaact gctacgcggc gtatcagagc ttccgtatct
    10321 gcttcttctg actcttctac aataagaatc tcaccgccta tttcctctga aaggtgtatt
    10381 aatcggtaaa gaaaggaaga actatttctg acatgaactt gcttgcctat taaatctacc
    10441 tggtttctga ttaattgagc ctcaatttcg tggagtttca tttgaggcca gttttcgggc
    10501 tttcgttgaa tcaggacctg tcgggttgtg tattgattcc ttgtaaattt tacgatcttc
    10561 ttcctttcag aagtaaccgt taagctgtag gcaattatat caccaacacc ggtattaagc
    10621 atcctaaagg ctttgtcgat gcttgtggtt tgaataagct ttaggtttac tccgaaatca
    10681 tcactaagca actttagcaa ttcatattca aatcccatcg gttgtccttt ataaacaaag
    10741 aaacttgttg agctattatc tataattgca atcaaagtgt cgcgttcaat aattttatca
    10801 aattcaaagt caactggaag tggtattgga tcccgggcaa cagaggtaga atccgaattg
    10861 ctttcggtga tggatttgcc gcagcctgtc agtagagaaa cacaggccat agcaagcaaa
    10921 gaaataattg cattgacttt atctgagacc aaattggagg gtttattaat acaataatat
    10981 agctatttaa tcatcaaaat tcaaaatttt atggccctgg ctatttactt aaatcggaat
    11041 gtctttttct tttccagcgg cggtgagacc agagccaatc ggaaggattt tctctaatcg
    11101 tcttttcgct ttcatgaatg aatttattaa gaatatcagt cttatcatcg tatggaggtt
    11161 ctcctaattt aagcgcctta acttgataat accctctttt aatcctggta atttttgcgt
    11221 agaacactgg gtattttgtg aacttggcca ggacatccgg ccctttgtaa aaagcggttt
    11281 cctgattgag gaatgaagac caataaatgt cagaattggg agtgggtgtt tggtcagcca
    11341 aaaggacaat cattcgcggg attttacttc ttcggacaat ttcccgggtt atattagaaa
    11401 ccggaatcgg aaaccctcca aatctcgatc tggtcctgat cataattttg tcgaagtatc
    11461 tatttgataa aggggaataa agaacatcag cgggcttgtt atggtaaact cctgcggcaa
    11521 gcaaaagcca ttcccaattc ccctgatgaa tagtaacaac aataactgat ttttcatctt
    11581 tgaaatatgg attcagctct tccggattaa catacttaac gcgtttaatt atttcttcct
    11641 ttgttatcgt tatctccttc aaaatttcaa caatgatatc tgcaagattg tgataaaatc
    11701 ttttggcaat tttacgaatc tctttttctg acatttcagg aaacgaattc ctgagattct
    11761 gaaaaactac ttttctacga tatcgaaaaa tatagaaggt taaaaaagaa aggatatctg
    11821 aaacaaagta caatgctcca aatggaaggt aggaaagtaa ccttagcaga aacatattta
    11881 tacagcgcct tgtttgattt cttcaaccac tgatggatcc aacagggtgg atacatctcc
    11941 caaatttgct gtttctccgg aagcgatttt cctcaggatt cgtcgcatta tttttccgga
    12001 tctggtttta ggcagcccgc ttacaaactg aatcttgtca ggtttagcga tgggtccgat
    12061 tgtttttgaa acggtatcca gtatctcctt ccgtagcctt ccgtgatcgt gttcatgatc
    12121 atatagaatc acatacgcat aaataccctg tccttttatg tcatgtggat aaccaaccac
    12181 ggcagtttca cagacttctg catgctcatc aatggcattc tcgatttcgg ccgttcccag
    12241 gttatgaccg gaaacaataa tgatatcatc aactcttccg gtaattctgt aataaccttc
    12301 gtcatctttc tttacgccat ctccggtaaa atacatgttt ttaaaagtgg aaaaataagt
    12361 ttgtcgaaat ctttcatgat caccatagat agtcctggca atagatggcc agggaaattt
    12421 aatggccaac cttccttcaa tgttatttcc gtgaagttca ttccccttat catccattat
    12481 tgcgggctgg attcccggca ttgggaatga agcgaaagcc ggtttaagtt ttgttgctcc
    12541 ggcaataggg gaaatcataa aaccaccggt ttccgtttgc caataggtgt caacaatcgg
    12601 acatttgttc ttaccaacat ggttatcata ccaataccag gcttcttcgt taattggctc
    12661 acctacggtt ccaagaacgc gaaggctgga gagattttta ttctgaacaa acctgagatc
    12721 tgccttctcc aatgccctga tagccgtggg tgctgtgtaa aaaatattta ctttatgttt
    12781 atccactaca tcccaaaatc tacccatgtc cggccaggaa ggaattcctt caaacatcag
    12841 ggaagtggct ccggcactaa gtggtccata cacaatataa ctgtgaccgg ttatccagcc
    12901 aatgtctgct gtacaccaaa aaatttcctt ttccttgtat tgaaatgctg tgaggaaagt
    12961 gtaagtagta tataccatat atccggcaac agtatggacc atccctttgg gcttgccggt
    13021 tgagccggat gtatataaaa taaaaagtaa atcctccgag tctgtctcaa cagcatcaaa
    13081 agtgtctgat tttccctgca tcgcttcatg ccaccagata tcccggtttt gaaaccaatt
    13141 tatttgctgg ccggttcgct ttagtacaat tacgcgctct accgttttac actttgaaag
    13201 ggcctcatct gaaatttcct ttaacggaac agttttattt ccacggaaac ttccatccga
    13261 cgtaattacc attttgcagg cggcatcatt tattctgtct gaaaggctgg tggagctgaa
    13321 acccgcgaat acaatcgagt gtactgcacc tatcctcgcg cacccaagca tagccacagc
    13381 cagctcagga accattggca tataaatgca aattcggtct cctttttgta tgccattctc
    13441 tttcaaaaca ttcgcaaatt tgcacgcctc cctgtgcaat tgtgcatagg ttatttcctt
    13501 tgtgtcttcg gatggattgt tgggctccca gataattgca acctggtctc cgcgattttt
    13561 gagatgtcga tccagacagt tctccgttat atttaatttt ccacctgaaa accacctgac
    13621 ttccgctttc ttgaaatccc atttcagaac tttattccac tttcgcttcc ataaaaaatc
    13681 agtggcgata ccggaccaga attcttccgg attttcaata cttcttttgt gaatctccag
    13741 gtactcctga tgtgagttga tttgagtgaa catccctgat tatttgataa ttaaattacc
    13801 aaatataatg aagttaattc aatagctcag caattatagc agctcctgct cccaataagc
    13861 tccacaataa tcgttgaaaa ttgaacttat gttcgggact gctttcaaat acaatcgtgg
    13921 tagaaatatg caggaaatta ccgcaaacaa tagcaaacaa aataagaaag aaatcggatg
    13981 cgccggatgc taattcaaag acatggctga gtattagtcc agctggggat gccaaggcaa
    14041 aaatgaacag gtaccccagg gcaacagttt tattttcaac agagcatatc aggaccgaag
    14101 acaatgccat tgaagctgga attttatgca gaacgatacc gattagcaga tttcgaactg
    14161 aaattgctac ttgcgcgttt tcaggaaacg ccaggattga gccttccaga aatgaatgga
    14221 tggataatcc gagcaatagg aaaatactaa aggaaaatcc gtgcttttgt ccaatttgat
    14281 gtttatgaaa atggccatgt tcggcaccat cggttataaa ttccaggatt tgcaggagga
    14341 agaatcctgc caagacaaaa agacctgcct taaacgcccc taattgtgat tgaaacaagt
    14401 ccgggaggat atgtaacaaa ctaatgctaa gcaaaaaacc tcccgcaaac gaaatagtaa
    14461 gcctgaaatt ctcttgcttc caattaaccg ggctatagag tagaaccacc ccggataagc
    14521 cggcaagtaa gagaattatt agatttacaa tcattgatcc gaattttaaa atttcacaaa
    14581 gatacctttg actcctttgc tatggtagtg ggccaatgtt ccatcaggat ttgaaaagat
    14641 cagaattgtc tcgagttctg aattattctc aacaatacta atcgatttct ctaaccccaa
    14701 gaccataaaa gccgttgcaa aagcgtctgc ggtcatgcaa tcactggcaa taacagtggc
    14761 gctaagcaga ttactggtta ttgaataacc ggttgcaggg tttattgtat gcactattct
    14821 ctgcccgcct tcatcaataa aattatagta gttacctgat gtcgccattg atttattttc
    14881 aagtgaaacg gtaccataca ggggttcttg tgcaggatca ttagccgggt caatgatgcc
    14941 aattttccaa tactcaccat tttcattttt tcccgcacat ctcacttcac cgccaatttc
    15001 aacgaaaata ttttcaagtc cgcgggaaat aaggaattca aaaaccacat ccactccctg
    15061 gccttttgct atagcgctga aatcaagttg aatgttcttt ttctttttcc acactttggt
    15121 tttgtcaaat tcaatatttt gaaaatagac cagttggcgc agtgaatcca ccatggcgct
    15181 atccagggtg attttcatac caggtccaaa tccccaggca ttaacaagag gacccacggt
    15241 agggtcaaag gctccatttg ttatttcgaa aacttcccgg cttttttgaa gcacaggcag
    15301 aaagtagggc gaaacaaata caaaaacaga atctgtatta aacttgctta cttccgaccc
    15361 ggaccggtaa tgattcaatg actgattaaa tgtctccagt agtgagtcta tctgaggctg
    15421 aaaattttct ccggaagagc ttagatattt tacggtgtat tttatgggac ccatggtggt
    15481 tccctgaagc aaaataagtt gaggtttctt gctttcacga tacttccata ccacgaaaac
    15541 cacaagaatc aataccagcg gataaagtct gcttttatga aataatcttt tcaaagttgc
    15601 ctgaataaac taaggaacaa tatttacaca ggcaaaaata atcttttcgc gtagtctgca
    15661 ttgagtgctt cacttttaat atcattttat atgcttattt tagtagaatt aattggagta
    15721 aatgcgagaa gattatttaa gtgcagggga tgaaaatctt aatccagctg ataaggagtt
    15781 ggaaaaagcc ttacgacccc tttctttccg tgatttcacc ggccagttta aagtcgtgga
    15841 aaatatccag gtatttgttc aagctgccag aaaacgaggt gaagctttag accatgtttt
    15901 attgcatggc ccgccagggt taggtaaaac aaccctgagt catataatcg caaacgaatt
    15961 aggcactaac atccgcatta cctcaggacc tattttggat aaacccggag atctggcggg
    16021 tttgcttacc aatcttgaaa acaatgatgt cttatttatt gatgaaatcc accggctgaa
    16081 ccctgtggtt gaagagtatt tatacgcagc tatggaggat ttccggattg atattatgct
    16141 cgatagcggg ccaagtgcca ggagtattca aatcacactt aatccattta ccttaatcgg
    16201 cgccaccaca aggataggtt tgctcacctc tccgcttaga tccaggtttg gaatcacttc
    16261 ccgtcttgag tattatgatg ctgatttatt aaatataatt gtcaggcgat cttcagcaat
    16321 tctggaagtt gaaattgata atgatgcggc caaagaaatt gcggccagaa gccggggtac
    16381 tccacggatt gccaatatgc tgcttcgccg taccagagat tttgctcaaa ttaaaggtga
    16441 tggtagaatt acgaaagaaa tcgctgacat ggcgctttct gcccttgatg ttgataaaaa
    16501 tggactggat gatatggata acaggatact ttcaaccatc atcaggaaat tcaaaggagg
    16561 cccggttggt atttctacaa ttgccacggc ttgtggcgaa gaagcggaaa ctatcgaaga
    16621 agtttatgag ccttttctga ttatggaagg ctatatcaaa cgaacagcgc gcggcaggga
    16681 agctactgaa aaggcttata aacacctgaa tgtactactg cctggaaaat tacctggctt
    16741 gtttgacgca tagaattatg tttcaattca atattttcag aaaacaatca agaggtcttg
    16801 cccccggctc cagaatattt gtaggtgatc aaaagatgga tgttccccgg accaccgtca
    16861 tggattttaa tccggaagat atacaagaga aagagattgt ggatgttgcc ggtttgggag
    16921 agtacaaaac gaccaataat tttacgtgga ttaatgttga cggtgtacat gacccgattc
    16981 tgatagaaaa aattggcaac gtgtttgata ttgattccct ggttcaggaa gacatcatga
    17041 acactacaca cagacccaag atggaagagc atgaagatta cctttttata gtgatgccga
    17101 tggtgtggat ggatgaaaaa agcaagaagg aaaattcaga acaactatcg ctaataattg
    17161 gacagaacta tctgatttca ttccaggaga aaacgggaga cttttttgag ccgcttagag
    17221 aacgtatcag gaaaagaaaa ggaagccggg taagaactct gggaatagat tacctggcgt
    17281 atgccataat ggatatcgtg atcgataatt acctccattt gattgattta cttggcgatc
    17341 agattgaaga cctggaggat gacgttttag atgacgtgga taatgaaatc ataagcaaga
    17401 taaatggctt taaaagagac attaactacc tgagaaaacg agtgcggccc gtaagagaat
    17461 ttattattct tttatcaaaa agtgaatcgg atctgttgag tcacaccacg attccattta
    17521 tgaaggacct gcatgatcac attcagaatg cttatgagaa tgtggaaacc taccgcgaaa
    17581 tgctgactga ttatctgact ctgtatcaca ccggcgcttc caccaaactt aatgatatcc
    17641 tcaggatcct gacaatcttc tcagcaatct ttattccgct gacttttatc gccggcgttt
    17701 acggtaccaa ctttcaatat tttcctgagt tacaacttcg ctatgcatac cccatttttt
    17761 gggcagttct catcatggtt acaattggca tgtttatttt tttcaaaaga aagaaatggc
    17821 tttgagccgg aaagaacgta attcaaaaat aattaaagag aaggcagctc tcctgggatt
    17881 tgatttttgc ggcatttcca gggcggaaaa actcaatgat gaggaaaaga aacttgaaaa
    17941 ctggctgaag caaggtatgc acggggagat ggcttacatg gaaaatcact ttgacaaacg
    18001 cctcgaccct gccaaacttg tgccaggcgc caggtctgtt gtttcgttca tttacaatta
    18061 ttatcccggg aaaaaattag ctgctgaaaa taattacaaa attgcaaagt atgcttacgg
    18121 aaccgattat cattttgtaa ttaaaaagaa acttaaattg atgctgggag aattgcgcaa
    18181 agaaattggt gattttgaag gcagaatttt tgttgattca gcccctgtaa tggagcggca
    18241 gtgggcggca aaatcaggat tgggatggat tggcaaaaat agtttgttgc tcaatagaaa
    18301 tatgggtagc tttttctttc tggctgtcat gatttccgat cttgaactaa ttcctgatgg
    18361 tctcattaaa gattattgcg gcacatgcac ccgttgcatg gatgcctgcc caacagatgc
    18421 aattccaatg ccctatgtag tggatgggtc cagatgtatt tcatatttta ccatagagtt
    18481 gaagaatgag ataccagtgg attacaaaga aaagtttgaa aattggattt ttgggtgtga
    18541 tatctgtcag gatgtttgcc cttggaacaa attttcaaat cctaacaatg agaacgaatt
    18601 caagccttca aaaaatttaa gcaacatgaa taaaaatgat tgggaagaat tatcagaaga
    18661 cacatttcag aagttattca gaaaatcagc ggttaagcga acaggtttta gaggattaac
    18721 cagaaatatt tcctttgtcg cgaaggaatg aattaaatga aaaaaacccg gacggagccg
    18781 gggcttttaa tatgaaaaaa actaataaaa ttaaatggct acaaagcaac gttcaagtac
    18841 aatgctatga gtttttccaa aattctcgta gcaccattgc tttaattcca ccagttctga
    18901 gcgaaccagt gtcaagatcg cttttctcaa ttccttctcg aaaagtttgg catcaaagct
    18961 cacctttcta agaattgttt taacatattc caacattggc tatgggatta aatttattag
    19021 cgaaaatttg aaatttaagg cctcttttag cattattcaa aatactaaaa aataaaccct
    19081 gttcaaaata aaacgttaaa tttgtggtga gggttgtggt tccggaattt ggcgataaat
    19141 gaaattttcc aaggttttct tacatataat tattgcattt ttttcagcat caattgcctt
    19201 tgctcaaaac gagaaagtta caatagagtt tgggcctgaa gaaattgcat ataatgacat
    19261 gttcacaata actataacag tacaaaatgg ccggttgcgc agttatgata attttcctga
    19321 tatgcccgga tttatcaaaa ggggaacgtc atcatcatcg aatacgaaca ttataaatgg
    19381 ccaggtaagc accagccaga gcatcaccca gaactatgtt cctgaaaagg aagggaattt
    19441 cagtgttcct ggcttcaaaa tgaaggttaa cggaaatgat cactcccatc cggggcaaaa
    19501 aattaaggtt gggccggctc gtgagaagcg tgacccattt cgtgacccat ttgatgacat
    19561 tttcggtcgc aggcagcaac agcagcctca ggattttgtt gatattgaag atgaagcctt
    19621 tcttgcttta acaacagaca agtcggaagt atatcttgga gaagggttca atgctacact
    19681 tgccttctat gtggctgaat ctaaccgggc tccactgcag tttcatgatt taggcaggca
    19741 actttctgag attctaaaaa gattgaaacc ttcacattgc tgggaagaga atttcaatat
    19801 tgagaatatc aatggcgaaa ctgttttgct cgataataag cgttatacac agtataagat
    19861 ttatcaggct gaattctatc cattgaatct ggatgaaatt aaattctctt cagttggttt
    19921 ggaaatgatc aaataccgtg ttgcacggaa tccttctttt tttggaagga accggcagga
    19981 agattacaag acattcttca gtaaacctaa aagcgtaaaa gtacgtgacc tgccacctca
    20041 tcctttgaaa gataaagttt cggtgggcag attcaggctt gaagagcgca taaatacgaa
    20101 tcctgtaatt accggcagca gttttaccta tgaattcaat atttacggtg aaggaaacat
    20161 ttcagcgtta aacaaaccgg agatcaaatt ggatgacaac tttgatttct actcgccaaa
    20221 tattgaacaa aatataattc gaagaagcgg caaggtaacg ggttcaaaat tatttaccta
    20281 ttttggaatt cctaatgagc cgggagaata tgatcttagt gactatatct cattgatcta
    20341 ttttgatccc aaccgggaaa tttatgatac actaaaatcg tcagttattt taactgccat
    20401 tggtgaaagt aaaaaaaacc aaacaatttc cgcttcagat ttaggatcat tttatgacag
    20461 actcaacacc ggggacaata ctctccgcag aaggaatgcg ctggcagaca tcaggctatt
    20521 tgctaacgtg atgatattgg tgctggtggt tggagggtct gccttgattt tcaaaaaata
    20581 aactttatgt aatgaaaata ttaattagcc tccacctggc agtgctttta attatttgga
    20641 caaattctgt caggacttct gaattgatta atgaaccgga atcaaaaatc ttgcttgttg
    20701 caagtacacc ggcagacgat gccgtaaagg ctatgcttgg aattccaata gacttagaag
    20761 ttgatttcat aaggtgggaa attaccttca gccaaaatca aagtccggat aggtttattt
    20821 tgaatttaat ttatggaaaa gctcaaccca acacacttgg ctttgtggca ggcggtaaca
    20881 aaaaaacctt taccggcact tacctgacca aagtaaaaag cgatggaaac ctgcaggggg
    20941 aaatatacga attaagtgaa gacgatgcat ctcgacaaat ttcactggta aaacttagcg
    21001 aaaacctgtt tcacctggta actcctgaca ataagctatt cgttggaaac gggggctgga
    21061 gttatacctt taacaggaat aataatcaat caaatatttt cccctctgaa tttctaagat
    21121 tgcaatcttc aattccgcga tactcaacca atgttatcac ctttgacggg cgaactcctt
    21181 gttacccatt ctctattttc aaagaatcgc aaatatccgg tgattgcata aaaacaaaat
    21241 ggagtttgat tttgcatacc gatccggaaa cttcattacc aacgggttat gaactgaaaa
    21301 ccacctttaa ccggacttcc acaattcaag gcaaatggtc tgtaacaatg ggttttggag
    21361 ccaatccaca ggctgttatt tttcaactta acccggatga tccgcaaaag tcagtttact
    21421 ttttagttgg agatgaaaat gttctctttt tccttgatga tgaaaaacaa tttctgcaag
    21481 gtaacagtga ttttagcttt acgcttaata aaagggaata aggagagata aattgaatag
    21541 tattgtttcg gtcacgtgag gaactcccgg caaagcccgt tcagttccga tatccgtaag
    21601 tattcgcttt ctagcaattc tctctttctt tttatagctt taagaccgct ttaataatca
    21661 tgttcaattc cttcggacac gtattcagaa taacaacatt tggagagtcg catggtcccc
    21721 aaatcggagt gattatagat ggatgccctg caggattaaa gatagatgcg cagtttatcc
    21781 agtcagaact gaaccggaga aagccagggc aatcaaaaat tacaagtgcc cgaaaagaat
    21841 cagatgaatt tgaaatagta agcggtgtct ttgaaggcat gacaacaggg atgccggttg
    21901 caatcctgat tccgaacaag gatgcaaaaa gcaaagacta ttctcatctt gctaaatctt
    21961 acagaccttc tcatgcggat tatacatatg cggccaaatt cggaatcagg gattacagag
    22021 gcggtggcag aagttctgcc cgggaaacgg ctctatgggt tgccgccgga gctattgcca
    22081 aattgctttt aataaaaaca aagattgata ttcgggctta tgtttcttcg gtgggtaaca
    22141 ttcatgcttc ccattactcc aaactgaatc taaaaaaaac cgatagcaat attgtccggt
    22201 gtcctgataa ggttgctgct aaaaaaatgc aggctaaaat tgaagcagtt aaaaaatcag
    22261 gagataccct gggaggctca attacttgtg tggtgaaaaa tactcccgtg ggtcttgggg
    22321 aaccggtctt caataaattg cacgccgctt tgggaggagc gatgcttagt attaatgcag
    22381 ttaagggttt tgaatacgga agtggatttc agggagcaac catgaaagga tcagaacata
    22441 atgatatttt cattaatgag aagggagtaa ttaaaactaa aacgaaccac agcggaggca
    22501 tacagggtgg catttccaat ggccaggata tctatttcaa agtagctttt aaaccagttg
    22561 ccaccataat gcaaacgcag gtgagcatta atgagaagaa acagagaata aaattgcagg
    22621 gaaaagggcg gcacgacccc tgcgtagtac caagagcggt accgattgta gaagcattga
    22681 ccgctttggt acttacagac ttttatttaa taaatcaaac tcgtaaattt tcaagataac
    22741 caattttcag tatgaagaag atccctttgt atgtaaaaat attgattgga atgggcttgg
    22801 gtgtgatctg gagtattatc gcccagaact atgaactgat ggatttcaca aatgactgga
    22861 taaagccttt cggcactatt ttcatcaatg gactaaaact gatagccgta cccttaattt
    22921 tagtttcatt aattgatggt ataagtggtt taaaagatat aacaaagctt agccgcattg
    22981 gaggaaagac tattgccttt tatttgttta ctacggttct cgcaatcact ataggattgc
    23041 tgctggtaaa tactataaag ccaggtaaat ttctaacccc ggaaaaacgg gaagaactgc
    23101 gggaagccta tgcatcagac gcgcaactaa gagttacgga agctcaaagt gaacaagaga
    23161 agggtcccct tcaaatgttt gtgaatattg tcccggataa tgttttccag tcaatgacag
    23221 ataaccgggc aatgcttcag gtgatcttct tttctttcat gtttggaatt gcgatgattt
    23281 ttgttgggga agagaaagtt aaaaatgtga aaggattttt ttccggtgta aacgtcataa
    23341 ttctcaaaat tgtggatatc ataatgaggt attcacccat cggggtattt gcgctactgg
    23401 ggagtttgag tatcgacgct tcgatgttca aggcattagg cgtttattct cttacagttt
    23461 tggcaggact tgccctaatg gtagttgtat tatatccctt aataatacgt atttttactc
    23521 acattaaata ccggcatttt ctgaagggaa tttttccggc ccagatactg gcattttcca
    23581 ccagttcaag tgcggcaacc ctgccggtaa ccatggaaag agttgaaaaa aaattaggtg
    23641 tatcggaaga ggtgagtagt tttgtgttac cgttaggcgc cacggttaac atggatggaa
    23701 cggctatcca ccaggcagtg tctgcagttt ttatcgccca ggcatttggg cacgatttgc
    23761 aattggcaga tcagttggtt attgtcctta ccgcaacgct ttcttccata ggtgccgccg
    23821 ctgtacccag cgcggggtta atcatgttgg taattgtact aagtgcaatt ggtgtcgatc
    23881 cggaaggtct cgcactaatc attgctcttg accgacccct tgatatgtgt agaaccgtag
    23941 taaatgtaac cggggatgcc accgtagcca ccttagttgc gagttccgaa ggagaactgg
    24001 caactggcat agatgaagcc cgcttaaatg ctgatgatga ataaaaatac cttatttttg
    24061 taaagaatta atcctgttga aatgattgaa gcgaaagaaa aagatccagt gagtatttat
    24121 atggagtcaa acccaaatcc aaactcatta aagtttgtgg caaataaaat gctcattcag
    24181 gagggaaaga gttatgattt cccggatatt gaaagtgcgg aatttgcccc actcgcccgt
    24241 gagttgttcg cgctggatta tgtggatagg gttttctaca tgagcaattt tgtgaccgtt
    24301 acaaaaaaag aaaacattga atgggttgaa attcaaaatg agttaaaatc aaaaattaaa
    24361 tcattcttag aatcagataa gcctttactg actaaagaac cggagccata tcaacctgaa
    24421 atcgaaacag ttgccgattc tgataccgta gccaggataa aagaggttct tgatgaatat
    24481 gtaaagcccg cggttgaaat ggatggcggt gctattcaat ttgaatcctt tggtgaaggc
    24541 gtgctaaaag taaatttgca gggaagctgc agcggatgtc cgtcatcaac cgtcacgctg
    24601 aaagcgggca ttgaaaatct actcaaaaaa atggtgccgg aagttaaaga agttcaggca
    24661 ctgggagttt agtctttctg aactattttt gaaggatgaa aaagtcggcc gccgaagccc
    24721 ttgcatgcgt acaatccgga aatcatatat tcatccatag tgttgccgct gcacctcagc
    24781 atttaatcag agcactggct gacaggtcat cagagctgcg tgatgttcaa atctaccaat
    24841 tgcatactga aggtgttgcc ccatacgcgg acccgggcat ggagaaatct tttcacacca
    24901 attgtttttt tattggagcc aatgtaagga aagcggtaga agagggccgt ggcagttaca
    24961 ttccggtttt tctttccgaa gtaccgtatt taattcgaaa agaaatcgtt cgaatagatg
    25021 taagtctaat caccgtatca ccgccggaca agcatggatt ttgcagcctg ggaacttctg
    25081 ttgatgcaac ccggtctgca atcgaaatgt cagagattgt aatagcgcag gtaaatcact
    25141 atatgcccag gacacatggt gatgcgcaga tccatatcgg caaattgaat ttcattattg
    25201 aacatgatga accgctggta gaacatggac cgcctgtaat tgatgaatta acgcgtaaag
    25261 ttggaattca tgtagcaagt ttgattgatg atggtgcaac attgcaaatg ggaatcgggg
    25321 ctatacctaa cgcggttttg ggtcagctta aaatgcataa agacctgggc atacacacgg
    25381 aaatgttttc cgatggaatt attccacttg tggaaagcgg cgtcatcaca ggaaaatata
    25441 aagtgaaata tccaggccgg gttgttgcca ccttcgtgat ggggacaaaa aagctttacg
    25501 attttgtgga tgataacccg ttaatggcat tgctggaatg ctcttatgta aatgacacta
    25561 agataatcag acaaaacccg cgagtcacgg caataaactc cgctattgaa attgatttaa
    25621 ccggtcaggt ttgcgctgac agtattggaa gccggatgta ctccggcgtt ggcggacaaa
    25681 tggatttcat tcggggggcc tctctttcac aaggtggtaa agcaatcatt gctttacctt
    25741 ctattacttc gcgtggtgaa acgagaattg tgccacaact gaaagatggt gcaggcgtag
    25801 ttaccacccg cgcacacgta cattatgtag ttaccgaaaa cggcatagcg catttatacg
    25861 gaaagaccct taaagagcgg gccaaggaat tgattaccat cagtcatccg gatcaccagg
    25921 aaggattgga cagggttttt catgagagat ttaagtcatt gatgtagtat caattgcaga
    25981 tcgtcaattt tcaatcgtaa tgaaaatgga ataaattgca tattgatctt ggcacattga
    26041 aatgggggcg ttagctcagc tggtttagag cgcttgcttg acaggcaaga ggtcacaggt
    26101 tcgaatcccg tacgtcccac aagaataaac ttatcatgaa aattagcaat ccaggagatt
    26161 ttaaaatttt tcctctcgca gattatcgcg ggaaacttcc tcaaccattt tttgggttca
    26221 gaagggcaat tcaaaaaccg atcatcgaat caaatttcct cccattgtaa ttacccgcca
    26281 ccgttgaaat aatagtaaag ggaaacattt ttaatcaata gttacatttg taaccatttt
    26341 gataacaccc aatgggatta gaaaatatag cttatggccg gctgtttgcc cgaatcgcgg
    26401 aggcttactt aaatgtcctc tccagaaagg tcgaaccttt tggactcgac cggtattttg
    26461 gcgcattgct atacattgtt gaaaattctg gtaaaataac ccagaaacaa ttgggccgtc
    26521 atttaagtaa agataaagtc tctgtgctcc gatgtgtgaa ttacctgatt gcccagggac
    26581 tggtactcaa gaaaatgaat caacaagaca agcgcgaatc ttttcttgcg gcatcggaaa
    26641 aaagcaaaaa attagctcca attatctctg tggcgataag agatacaaat aacattctgt
    26701 ttaataattt tcaaaaaaaa gaatttaaag aatttgaaac cggattaaaa aaattattaa
    26761 cggtggtaga taagatgccg gatccggatt ttattattag agctgaaaaa aagaaaaacc
    26821 tggtttttga gagatgaata agaaattact tttagtgacc ggaatgcttg gcatattgtt
    26881 tcctggaact tcatgtcagg agggaaaaca acaagcagta cctattagca ggtctttact
    26941 tcaggttgaa gctatagtgg caaaaccacg acgttttgaa aataaattta cagcgactgc
    27001 taatctgctt gcttttgaag aagttgaatt gcgggctccg gtttcaggaa cagtattggg
    27061 tatttacttt caggaagcac agaatgtaaa gcagggtgat ttgctggtta ggcttgatga
    27121 ccgtactctt aacgctcaaa taaagggatt gaaagttcag cttgaagcat ctctcagtga
    27181 tctgagaaga aagaaggaat tgctaaaagt tgaaggagtc agcgaagagg aagtagagca
    27241 atcccaggca gttgtagatg gaattaaatc aagcatggat gagcttacag tttctgtgaa
    27301 tcttgcgaat ataagggctc catttgatgg cagagtcggg atgagaaatt tcagtatggg
    27361 ctcttacttg tcacaagggc aaacgataac ccaacttgtt cagactcaaa aacttaaggt
    27421 agattttaat cttgcgagcc gatatctgga tgaggttaag gttggaaaga aaataaaagt
    27481 aatatcttca tcagataccc tggaagctga agtttatgcc attagtcctt ttgtaaacgc
    27541 tacttcaaga actgcacagg tgcgagccat catccaaaat aataaccgtg gattcattcc
    27601 cggtgacttt gccgaagtgc aggtagcact taatgttgat aatgaagctc tggttgttcc
    27661 cacagatgtt attattcctg atttgaatgc ccaaacattg tttatttata gaaatggcag
    27721 ggccatgaga actgaagtct tacttggatc acgaactgat actgaagttc aaatcttatc
    27781 cggcctaagt ccgggtgata ctgttttaac aaccggtttg cttcaggtaa aggatttaac
    27841 tcaggttgag ataacagaat ttgttaaacc ggtaactcta tgaccctatc agaattaagc
    27901 attaaacgac ctgtgctggc aactgtgttt gctatcacca tcgtgttatt tggttttgtt
    27961 ggtatgtatt ctcttggtgt cagggaatat cccagcgtag atccacccgt tgttacggtt
    28021 agcaccagct ataccggggc gaatgccgga attattgaat ctcagattac tgagccctta
    28081 gaggaagcaa ttaacagtgt tgcgggaata aagtcgcttc gttcaaccag cagtgatgga
    28141 agaagttctg taacaattga gttcgaaact gatgttgaca ttgaagatgc cgccaatgat
    28201 gtgagagaca aagtgtccca ggcagttcga agtctgcctc ctgatgctaa tcctccggta
    28261 gtatcgaaag cagatgcaga tgcgcaaact attttttccc ttaccgtgca aagtgacaaa
    28321 agaacgctat tagaactaac cgaacttggt aataatgtgt ttaaagaaag gcttcaaacc
    28381 attccgggag tcagcagtat tcgtgtttgg ggtgaaagaa aatatgctat gcgcattatc
    28441 cttaatcctg ataagctgga agctttcagc ttaacgccat tggatattcg a
//
LOCUS       DASWHX010000121        18365 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_1211131, whole genome shotgun sequence.
ACCESSION   DASWHX010000121 DASWHX010000000
VERSION     DASWHX010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 18365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18365
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1211131"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(60..578)
                     /locus_tag="VGA21_08465"
     CDS             complement(60..578)
                     /locus_tag="VGA21_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020526632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX9650579.1"
                     /translation="MDDMSVEAFQAKVLPVKNKLYRFAIKLLQSEEDAKDVVQEVLIK
                     VWNKREELGHLKSFEAWCMTVTRNLSIDKLKSKHNRSRSFPEGFEVEEKRKGPHESLE
                     IDDAMKRVNQFIQELPEKQKQIITLRDIEGYTYNEISEILDIDLNQVKVNLFRARKSI
                     KENLLNINSYGL"
     gene            complement(675..2561)
                     /locus_tag="VGA21_08470"
     CDS             complement(675..2561)
                     /locus_tag="VGA21_08470"
                     /inference="COORDINATES: protein motif:HMM:NF036516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M61"
                     /protein_id="HEX9650580.1"
                     /translation="MKKIFTLVLILIVLSAKASIDNYTYIINLTKVVNDRVQVELRVP
                     QITSDTVIFYMPKIIPGTYQVSDYGRFVSDFKALDNKGKELAVLRTDDNTFVIPDASK
                     IDLISYWIDDTYDADMGEIVIDPMGGTNIEKDKNFVINTGGFFGYLDGKEDLPIELQV
                     VRSADFYGSTGLIVDKSGINPKSDKQFKKYPANSRIDIFKLDDYHHLIDSPLMYNRPD
                     TTSVFIENTQILISVYSLGNNVQSQFVAETMRKLLLAQKEYLGGKLPVDKYAFLFYFE
                     DLMKIADVQGALEHSYSSYYYLPDVDQNSMEQTLRDIAAHEFFHIVTPLGIHSEEIEF
                     FDFNHPKMSRHLWLYEGVTEYFAGHVQLKYEMVSLEDYLEMIRLKLIYSTGEFDDNLP
                     FTDLSQYTLDKYPDQYQNVYQKGALIGLCLDIKLLELSEGKYGLQNLLADLTEKFGKN
                     KPFKDEELFTIIEELTYPQVGEFLKSYVSGTLSLPYTEIFNLVGINHHPYQQTKTFTL
                     GNFGITLEGTSLVVEDISNLNEFGKNLGYQKGDVLYKFQGTHVPENPYQISTYIDNIL
                     SSMIEGEEFSVTVLRKDEKGKQAEVELKAEAMKFDAYAVHVLEINQNPSEKQLKLRQN
                     WILP"
     gene            2871..4862
                     /locus_tag="VGA21_08475"
     CDS             2871..4862
                     /locus_tag="VGA21_08475"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX9650581.1"
                     /translation="MNARLTIIFAMLLAACTQQREREIEGFLEEGSDLRPYQMVNGTL
                     EVKTPQSYKINLDSGTYVYGIVDQLSVDAALKILSPSGKELVKFDGPASGHEVFFFDT
                     GEAGLHVIEVAPFKEDSGNYSIVIKRIEAIAEVPEERVGQLLAPFDDPEVPGAAVGVY
                     HDGALVYKKGFGSANLTYNIPFTSVTPTNIGSVSKQFTATAILLLEQEGKLSLDDDVR
                     KHIPELKDFGHSITIRNLLNHMSGYREIYNTLPIIGWDGESFLQRDEAINLVNKQPSL
                     QVEPGSEFNYNNTGYILLAETVARVSGISFPEFMRQNIFEPLEMNNTQVRTDPREIIP
                     KSSMGYQNQDYGYGEVGDLFAAYGAGSIYTTIEDFGKWLQNFKTGKIGGLELITKLTT
                     RGITTKGDTLDYALGIGVGEYRGQKRFQHTGGDIAHRAILIYYPEIDAGLAIASNNAS
                     FPLNVSTKIADLFFGDYFDKAGETQLDSGEVEVSRELLETYAGKYKFDNSGLIITYIL
                     EDDGFFAQAMGQNRLKMKAKNDSSFAYEGIEASVVFYRDEEGIVSSATHIQGGENTMT
                     RLPPYDPTPEQLAEFAGKFFSEELETMYEISVKDSILVARHLLMEDISLTPAEEDSFS
                     GSVYFINELKFERDSNGKVLGFNVSNGRTKGVKFGKMRE"
     gene            4873..5076
                     /locus_tag="VGA21_08480"
     CDS             4873..5076
                     /locus_tag="VGA21_08480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650582.1"
                     /translation="MACRGTGHGWEDFRLNGPRSMVCPLTDHLGAIKRPDWMAWMRWG
                     WENQLEWPVGGQAMDEKFGKMRE"
     gene            complement(5124..5678)
                     /locus_tag="VGA21_08485"
     CDS             complement(5124..5678)
                     /locus_tag="VGA21_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011586553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX9650583.1"
                     /translation="MSKGAPFRNSRMKLNEVYFWTNTIKDWKHLLKKSKYEHLIIEQL
                     QWLKNRNKILIYGFVVMPNHLHLLWEMLEMNGKEMPHASFNKWTSSRFLKDLRINNKK
                     KIPDFVEKTSERNHRFWQRDPLAIQIDDRVKLEQKLDYIHLNPLQDHWNLATKPEEYP
                     WSSAKFYEKGIDDFGILTHYMDKF"
     gene            5903..6046
                     /locus_tag="VGA21_08490"
     CDS             5903..6046
                     /locus_tag="VGA21_08490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650584.1"
                     /translation="MRELLIYSILEIFVLNGLSGDRPWMRWGWENQLEWPVGGQAMDG
                     DGL"
     gene            complement(6263..7324)
                     /locus_tag="VGA21_08495"
     CDS             complement(6263..7324)
                     /locus_tag="VGA21_08495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650585.1"
                     /translation="MGKKYPQSAFTSLVVLNFVVGFSWIAYHNYQPKLLDAFELNTVG
                     EFLFYAQWLILFAVPGLAGKLSDNMLRRRNKYLIIYVTGITVAAMTFMSVALGVGIIN
                     LLSLRPLVPFLVIVWLISMNMFVAPANALLKNIAEQDRLPKLMAILIIGTEGVYALEP
                     VIIPLIEGMGAVLTFIAGGLLVTFAGIWFQRQLKKNPEIMSVDYEEKPDHKPLVFVAA
                     SIAFGSVFAFIIAVLPSLIGARLNVDGSTTVAAMLGFSTLIAWPVSRLSTKFSLQTLA
                     GTGWVMGGVSVLALLIISNESIVYMALIILGLSCSVLSVTTFPLALKNIPARNLALGT
                     GIFLGIAEGFEGFYQWLLQ"
     gene            complement(7702..10989)
                     /locus_tag="VGA21_08500"
     CDS             complement(7702..10989)
                     /locus_tag="VGA21_08500"
                     /inference="COORDINATES: protein motif:HMM:NF024974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HEX9650586.1"
                     /translation="MFYQKIVITILLLVPFPGYSQSNNSSLSFIENKGQWDTNYDFGS
                     WFSGGKLLVNSSGFIITLFDAEAIGNYHLMSHGLISESDERYVCNDNIQGHNLQLSFA
                     GSNPHSKFIAKQKKSAYYNFFLGKDKSNWVSKAFAYETLLYSEIYEGIDLQISSVNKN
                     LKYDFIIEPCGDPNQIIIEYKGSDLIGLENGDLMIQTSLGRVVEMKPIAFQIRGEQKI
                     NVPVLYQIENQKVKFDFPEGYDDSMGLIIDPLLIFSTYSGSMADNWGSSATPGEKGTL
                     YSSGVTNHFVGSTFSGTFPATPGAFQTTYGGIYDVSILKYDSLGQNLLYASYLGGSDN
                     DSPHSLIMDKDENLIVFGTTGSIDFPTTFGVISQNYSGGSVFGNDVINYNNGSDIFIA
                     KISKDGSTLIKSTYLGGGGNDGLNNTGGPLTKNYGDENRGDIITDDAGNIYISSVTWS
                     PNFPVVNSFSTSFHGGGTDAIVAKISPDLSTVVWAGYLGGSGYDASHTIKINQEGNIV
                     VAGGTTSTDFPVSAGAYQSSYGGSADGWISVISSDGLNILNSTYTGSANFDQVYIIDL
                     NEDDEVYLFGQTTGNIPVTPGRYSNPNSGQFVQKLSADLSTLIWSTVVGSGTQIPNIS
                     PTAFLVNDCNNLFLAGWGGLVNSSRFGWDSDTRGLPISADAFQKNTQGSDFYFMVLNE
                     EATQFLYGTYLGGTSSSVHVDGGTSRFDKRGIVYHAVCSGCFTASSDFPTTPGAYSAT
                     NNSINCNNAAFKFDLSLLKAQFRTNTLDFKSPNVSALCYPNAIVFENLSIGGEIFEWD
                     LGDGTQIFKTDTTAIAHQYQQPGSYKVQLKSVDQNTCIGVDSVTKIINIYGRDSQVQE
                     DDEICYNTEYQLMASGGIAYSWSDQTGAIFSNQANPVVMLTQTGQFYVDILESTGCFY
                     SDTVTIEVLEPIIPDFDLIMKNNCFERPVIELIDKSVYSVGDTVYFDFGDGQTSDLDQ
                     VTHSYDQDGFYTVKAIGKGKLCSYEKIIQVPIFTLSVPNVITPGIEDGKNDYLLVQFG
                     DGSETANAIGLQASLTVINRWGKVVFETNNYQNNWKAENSPAGVYYFVVVMDGYAECT
                     GWVEVIK"
     gene            complement(11101..11565)
                     /locus_tag="VGA21_08505"
     CDS             complement(11101..11565)
                     /locus_tag="VGA21_08505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650587.1"
                     /translation="MKFFDHSVSNSLQYITLILEVAGITLAFIEIKSPRTADKIEKAI
                     RGIQKFTERASYKVTRSHAGQTLITLFIILLFAAVVPTIWGLFTLPWYLWLLFGIVGG
                     IFGIILGLHLTVDFINSLNKFSNGKAIGALGICLASLGMVGEVYQILFIWFE"
     gene            complement(11597..12862)
                     /locus_tag="VGA21_08510"
     CDS             complement(11597..12862)
                     /locus_tag="VGA21_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010854063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX9650588.1"
                     /translation="MDKRSFIKSLALLGFGATSSLKVLANLTESVSHIPAEKLASDED
                     FWAKIRSAYKLRPDYINLENGYYCFLPEETLENFIRHVREVNYQGSYYMRTVQWDNKN
                     AMATKLATLHNCEPDELILTRNTTESLDMVIGGLDWNAGDEAVMAEQDYGAMLNMFKQ
                     VAKRYGVVNKIVSVPNHPKSDEEIVQLYESVITSKTKLLMVCHIINITGHILPVRKIC
                     DMAHSKGVSVMVDGAHAFGHFTFSMKDLDCDFYGSSLHKWLSVPLGAGILYAKKSAIP
                     KVWPLLAEGEREPDDISRLNHIGTHPVHTDLAVANAIDFHNMIGPKRKEERLRYLQTY
                     WTSKVRDIPGVVVNTPRDPARYCGIGNVGVEGMKPKDFADTLMEKYKIYTVAIDGAGV
                     HGCRISPNVYTTPKELDVLVKACMELAKS"
     gene            complement(12994..14742)
                     /locus_tag="VGA21_08515"
     CDS             complement(12994..14742)
                     /locus_tag="VGA21_08515"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX9650589.1"
                     /translation="MPDIITMKKFPCLLLIPFLTLSSCFKEPSAELIIINGKVYTFQW
                     PDPDLDGNPSPGAPFANGAWHPEAQAIAITGKLISNIGTTAEIQKLAGENTEIIDAEG
                     GTIIPGLVDSHTHVINLGQNLELVDLTGVETEAAAVALIVETSKNIPAGEWIIGKGWD
                     EGAWASQYPDKKMLSEMVPKHPVLMLSLHSFASWANQMALDNAGITKDTPSPPGGEVV
                     KDENNEPTGLFLNKGSDIIEAAIPQPANQQLKEYFLAGLIEMAESGYVAIHEAGVNRK
                     LLTAIMELDKEGKLPIRVYAMLSGDDEDLLSEWKQRGPYESASGMLIINSVKAFYDGA
                     LGSRGAKLLEDYSDMPGHKGTSGLDYRFKPDILAEMSTAGFQLCIHAIGDAGNRQTLD
                     FLEVLIEANPEIKNLRNRIEHAQVLHAEDFQRLAELNIIASMQPSHAVEDMVWAEDRV
                     GHERITGAYAWRTLRKQGTHIIFNSDLTGTDHNIFYGLHSSITRQNKSFEPEGGWHPE
                     EALRPEEALRAFTTWAAWSVFREDSTGILAPGKWADISVLTIDPFQTGNSDPSALLSG
                     EVYATIVNGEIVFRKK"
     gene            14983..17382
                     /locus_tag="VGA21_08520"
     CDS             14983..17382
                     /locus_tag="VGA21_08520"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9650590.1"
                     /translation="MRSIRIALRNIYRLDNISALNLTGLSVGIFSVIVLGLFVWTELS
                     HDTFLPDYKKIYRITTHYVLKGNEANWALNHGIMDSELEDKLPNLDHSTAFIKVQSEK
                     VFYSGDLSFTNSNGFYVDEDFFEVFNFNFLYGDLYSAIRQPNSIVITQSLAKAFFGKE
                     DAVGREIEIQEIDGKTPLVITGVVKDFPANSYIKFDFVISGKSYQPWDLYYSGKSGNM
                     FYVFFKTLNQIDAEELQQSVNAALGPIKKEFYGAEYEPSESFRLDNPVQRIDKIYFNA
                     KNEFEISAGGNQNYTILVGIVGVFILAISVINYINLTISSSFSKRKIKEFAVRKLLGS
                     SAGNLLAGFALEISIWVFMAIVGSLIILKFIFDLFPAATDLISSPFSYPGFYLIIIIC
                     WILLSLITSVFPASKFLNFNIVQSAKGNIQVGGSRTLNMRNLLVVFQVILTAILISNS
                     TLLFRQLNFLTTKNAGFKTSQIIEVKRSSNMTEGEWSNFMNKTKSLSFIKGVGGSQFT
                     HISDMSNAEIRTTVANSPFVKVAWNSVSHDFIPTLEIQIARGRNFSPDLRSDYKATVI
                     NETAARALNIDEPEGKFIIMWGDTLQVVGIVDDFHFQSFSKPISPLIFLLRPEGLTYV
                     AYIKFENDDTADLIGVLNDHWDSENLYALFDYRLMQDNFKSLLEKEEQMANIVASFTI
                     VAIILAILGLIGLVSHDAGRKSKEIAIKKILGAPILHIILLINRRFAFILIAGFTISL
                     PLAIWIGNYWLEVFVYRISISVWDVLVSVISIALLGVLTSSLITYNAASSNPVNVIRN
                     E"
     gene            17518..>18365
                     /locus_tag="VGA21_08525"
     CDS             17518..>18365
                     /locus_tag="VGA21_08525"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX9650591.1"
                     /translation="MNEYLSQENLNFILFEVLNCSGLTQAEYFKDYNEESLKLLLNSI
                     KDFADRDLFPSFRGMDNQPAYFENGKIVIHPAFGNIMKKIAALGLMGSTFKSEDGGLQ
                     LPSTIYTAAYFIMGAANNNVPGYSGLTSGAANLINTFGSLTLKEKFVSNMLSGKWAGT
                     MCMTEPQAGSSLSDIATLATPADEGSYKIKGQKIFISGGDHQFCENFIHLTLARIEDA
                     PAGTRGISLFVVPKFREENNEVADNDVIVAGEFQKLGQKGYCTTHLIFGDNNNCRGWL
                     VGEPNMG"
BASE COUNT         5436 a         3666 c         3772 g         5491 t
ORIGIN      
        1 cttcctcttc cagtgtagaa acacaggacc agtaccggtc cagtaacgtt tcaattcttt
       61 tagagtccat atgaattaat gttaagtagg ttctctttaa tacttttcct tgcccgaaac
      121 agattcactt taacctggtt gagatcaatg tccagaattt cactgatttc gttgtaggta
      181 taaccctcta tatcgcgaag cgtaataatt tgcttctgtt tttcaggcag ctcctggata
      241 aattgattta ctcttttcat tgcatcatct atttcaagac tttcatgcgg gcctttcctt
      301 ttttcctcca cctcaaaccc ttccggaaaa gaccttgatc tgttgtgttt tgattttagc
      361 ttgtctatgg aaagattcct ggttactgtc atgcaccatg cttcgaaact ctttaaatgt
      421 ccgagctctt ctcttttatt ccaaaccttt attaatacct cctgcacaac gtccttggca
      481 tcttcctcac tctgaagcaa ttttatagca aatctgtaca acttattctt caccggaaga
      541 acctttgcct ggaaagcttc aacactcata tcatccatat gacgaatcaa attatattat
      601 gttacaaatt gatcaaaatc tacctattaa tttaaggaaa atgaaaatgg atgattgggt
      661 agataagaga tgaatcaggg aagaatccag ttctgacgca gtttcagctg tttttcgctt
      721 ggattttgat taatttctaa tacatgaaca gcgtaggcat caaatttcat ggcctctgct
      781 tttaattcga cttctgcctg tttgcctttt tcgtcttttc tcaacactgt tactgaaaac
      841 tcttctccct caatcatgga ggacagaata ttgtctatat atgttgatat ttggtatggg
      901 ttttccggca catgggtacc ctggaattta tataagacat ctcctttctg ataacccagg
      961 ttcttcccga attcgttgag attgctaatg tcctctacca cgaggctagt gccttcgagg
     1021 gttattccga aattacccaa tgtaaaagtt ttcgtctgct gatatggatg atggttaatt
     1081 ccaaccaggt taaagatttc agtgtaagga agagatagtg tgccacttac gtatgatttc
     1141 agaaattctc caacctgagg gtaagttaat tcttcaataa ttgtaaataa ctcttcgtct
     1201 ttgaatggtt tgttcttccc gaatttttct gtcaaatcag caagcaaatt ttgcaaccca
     1261 tatttgcctt ccgatagttc aagaagtttt atatcgaggc aaagtcctat taatgcgcct
     1321 ttctgataaa cattttggta ctggtcggga tatttgtcga gtgtatattg acttaagtct
     1381 gtaaaaggca gattatcatc aaattcgccg gttgaataaa ttaatttgag tcgaatcatc
     1441 tcaagataat cctcaagtga aaccatttca tattttaatt gaacatgccc tgcgaagtat
     1501 tcagttactc cctcataaag ccaaagatgc cttgacattt ttggatgatt aaaatcgaag
     1561 aactcaattt cctctgaatg aatgccaagt ggagttacaa tatgaaaaaa ttcatgggcg
     1621 gcaatgtcac gcagagtttg ctccatgcta ttctgatcaa catccgggag gtaataatac
     1681 gaagagtaac tatgctctaa agctccctgg acatcagcaa tcttcatcaa atcttcaaag
     1741 taaaataaga aggcatattt atctacaggg agtttccctc caagatattc tttttgggcc
     1801 agtaataact ttctcatggt ttctgcaaca aattgagact gaacattgtt tcccagtgaa
     1861 taaacggaaa tcaaaatctg tgtattttca ataaaaacag aggtcgtatc tggtctgtta
     1921 tacatgagag gtgaatctat caggtgatga taatcgtcca gtttaaaaat gtctatcctt
     1981 gaattggctg ggtatttttt gaattgttta tcactctttg gattgatgcc gcttttgtca
     2041 acgatcaatc cggtggaacc atagaaatct gcggaacgca caacctgcag ttcaatgggt
     2101 aaatcttcct ttccatccag gtaaccaaaa aacccgccgg tatttataac aaaattttta
     2161 tctttttcaa tgttggtacc acccatggga tcgataacga tctctcccat atctgcgtca
     2221 tacgtgtcat ctatccaata ggatatcaaa tcaatttttg atgcgtccgg gattacaaag
     2281 gtgttgtcat cagttcttag aaccgctaat tcctttcctt tattatccag tgctttgaaa
     2341 tccgatacaa atcttccgta gtcagaaacc tggtaagttc ccgggatgat tttaggcata
     2401 tagaagataa ctgtatcgga tgttatttga ggaactctta attctacctg cacccggtcg
     2461 ttgacaacct ttgttagatt aataatatat gtataattat caattgatgc ttttgcgctt
     2521 aagactatta gtattaatac gagtgtgaat atctttttca taatttcgtc tgtttgctta
     2581 ttcttttcac ctttactttt aatctattcc tgatttcaaa cctcccggtc atgaattatt
     2641 ttgcaagaaa aaattagtta tcacaaaatt ttatcacagc ctgattcaga tgaaaagcgt
     2701 acactaattc aagttcagaa ttaattaacg taaaagtaaa ttggaaagtg gaaactatta
     2761 tttaaatggc acaataatct tttaaatcac aaaccacatt aaggccttaa ttgcttttaa
     2821 attaaagaag tttcgataaa tttatcaatt cacaaaacca atcgaattca atgaatgctc
     2881 gtcttacaat aatattcgca atgttgcttg ctgcttgtac ccagcaaagg gaaagagaaa
     2941 tagaaggatt tctggaagaa gggtcagatt taagaccata ccaaatggta aatggaaccc
     3001 ttgaagtaaa aactcctcaa tcctataaaa taaacctgga ttcgggcact tatgtatatg
     3061 gtatcgtgga tcagttgagt gtggatgctg ccttgaaaat tctctctcca tcaggaaaag
     3121 aattggtaaa atttgacggc cccgccagtg gtcatgaagt attctttttt gataccggtg
     3181 aagcaggact tcatgtaatt gaagtggcgc cttttaaaga agactccggc aattattcga
     3241 ttgtcataaa aaggatagag gccattgctg aagttcctga agaacgcgtt gggcagttat
     3301 tggctccatt tgatgacccg gaagtgccgg gagccgcggt aggcgtttac cacgatggag
     3361 ctctggtcta taaaaaggga tttggttctg caaaccttac ttataatata ccattcactt
     3421 cagtaacccc aaccaatatc ggttcagttt caaaacaatt tacagcaact gcaattttgc
     3481 tgttggagca ggaagggaaa ctttctcttg atgatgatgt cagaaagcac atacctgaat
     3541 tgaaagattt tggccactcg attacgattc gaaacttatt gaaccatatg agcggatacc
     3601 gggagattta taacacgttg ccgataatcg gttgggatgg tgaaagtttt ttgcaaaggg
     3661 atgaggcaat aaacctggtt aacaaacagc cttctttgca agttgaaccg ggttcagaat
     3721 ttaactacaa taataccgga tacatacttt tggccgaaac agtggcaagg gtaagtggaa
     3781 ttagtttccc ggagtttatg cgtcaaaata ttttcgaacc tttggaaatg aataatactc
     3841 aggtacgaac agatcctcgg gaaattattc ctaaaagttc aatggggtat caaaatcagg
     3901 attatggata tggtgaagtt ggagatttgt ttgcagccta tggcgcaggg tccatttata
     3961 ctactattga agattttggg aaatggcttc aaaacttcaa aaccggtaag ataggagggt
     4021 tggaattaat tactaaactg actacccgtg gaattacgac caaaggagat actttggact
     4081 atgcattggg tattggcgtg ggcgagtaca ggggccaaaa aagatttcag cacaccggtg
     4141 gtgacattgc tcaccgggca attttgattt attacccgga aatagacgcg ggacttgcaa
     4201 ttgcaagtaa taatgcttca tttccgctga acgtttcaac aaaaattgca gacttattct
     4261 ttggtgatta ttttgataaa gctggggaaa cacagttgga ttctggtgag gttgaggtga
     4321 gcagggaatt attggaaacc tacgctggaa aatacaagtt tgataactcc ggcttaatca
     4381 taacctatat actggaagat gacggttttt ttgctcaggc catgggccaa aacaggctaa
     4441 aaatgaaagc gaaaaacgac tcttcctttg catatgaagg aattgaagca agtgttgtat
     4501 tttacaggga tgaagaggga atagtcagtt ccgcaactca tattcagggt ggtgaaaata
     4561 cgatgaccag acttccgccg tatgatccaa cgccagagca gttagcagaa tttgccggaa
     4621 agttctttag tgaagaatta gagactatgt acgaaatttc ggttaaagat tcgatactgg
     4681 tagcaagaca ccttttgatg gaagatattt cgctgacgcc tgctgaggaa gattcttttt
     4741 cagggagtgt ttacttcata aatgaactga aatttgaacg tgatagtaat ggtaaggtgt
     4801 tgggatttaa cgtatcaaat ggccgtacca aaggtgtgaa gtttggaaaa atgcgggaat
     4861 aggaattcta aaatggcctg tcgggggact ggccatggat gggaagattt cagattgaat
     4921 ggcccccgat ccatggtctg tcccctgaca gaccatctgg gagcaatcaa acgaccggat
     4981 tggatggcct ggatgagatg gggatgggaa aatcagcttg aatggcctgt cgggggacag
     5041 gccatggatg agaagtttgg aaaaatgcga gaataggaat tcttgaatgg cctgtcgggg
     5101 ggacaggcca tggatgggag caatcaaaac ttatccatgt aatgagtaag aatgccaaaa
     5161 tcatctattc ccttttcata aaattttgct gatgaccatg gatattcttc cggctttgtc
     5221 gccaagttcc aatgatcctg caaaggattc aaatgaatat agtctagctt ttgttccaac
     5281 ttaactctat catcaatttg aattgcaagc gggtctcttt gccaaaaccg atgatttcgt
     5341 tcactggtct tttcaacaaa gtctggtatt tttttcttat tattaattct gagatctttc
     5401 agaaatctgc tgcttgtcca tttgttaaat gaggcatgtg gcatttcttt cccattcatc
     5461 tcgagcattt cccaaagcaa gtgcaaatgg tttggcatca ccacaaagcc atatatgaga
     5521 atcttattcc tattcttcaa ccactgaagt tgctcaataa ttagatgttc atatttactc
     5581 ttctttagca aatgcttcca atctttaatg gtatttgtcc agaaataaac ttcattcaat
     5641 ttcattctgg agttacgaaa gggtgcgcct tttgacattc ttgaatttac ttattaaaga
     5701 tgggtagaaa atcaccgtga atgtgatcga ttaaaaactg gtctgatagg agagtgcaaa
     5761 ccgaataaga gccagtgaaa ccaagaacaa aactcctaag tgaaagaacc atcagactaa
     5821 agacttaatg aaacatttgc gaatattaat gaatattact tattatttgt atagtaatgg
     5881 aattttaaaa tccttataaa ggatgcgaga attattaatt tattcaatat tagaaatctt
     5941 cgtcttgaat ggcctgtcag gggacaggcc atggatgaga tggggatggg aaaatcagct
     6001 tgaatggcct gtagggggac aggccatgga tggggatggc ctgtaggggg acagtccatg
     6061 catgagagat ttgggggacg aaacatagac tcagattgaa agcttggatg gaatggcctg
     6121 tcggatgaga gactcaaatt gaatggcctg tcgggggaaa ggctatggat ggctagtcgg
     6181 tgggaacgat tcaaagactc ggattgaatg gacgggtctc acatgagatt acaaagatta
     6241 cattagttaa gttaagcaat tattactgca acaaccactg ataaaagcct tcaaaaccct
     6301 cagcaattcc aagaaatatg cctgtgccca gtgcaagatt tcttgctgga atgttcttca
     6361 gagcaagtgg gaatgtagta accgagagca cactgcagga caatcctaaa atgataaggg
     6421 ccatataaac gatgctttcg tttgaaataa ttaagagtgc aaggacagaa acacctccca
     6481 ttacccaccc ggtgccggct aatgtttgaa gggaaaattt agtggataac cgagataccg
     6541 gccaggcaat taaggtagaa aacccaagca tggcggcgac agttgtgctt ccatctacat
     6601 taagcctggc accgattaac gatggaagca cagctataat aaaagcgaaa acagagccga
     6661 atgcaattga tgccgccaca aaaaccagag gtttatggtc gggcttttct tcatagtcta
     6721 cgctcatgat ttcaggattc ttttttagct gtcgctggaa ccaaatacct gcaaatgtta
     6781 cgagcaaacc tccggcaata aaagttaaca ctgctcccat cccttctata agtggaatga
     6841 tcacaggctc aagggcatat acaccttctg ttccaatgat cagaattgcc atcagcttcg
     6901 gaaggcgatc ctgttcggct atatttttta ataatgcgtt ggctggtgct acaaacatgt
     6961 tcatggaaat cagccaaaca attacaagaa atggtaccag cggtcgcaaa ctcagtagat
     7021 taataatgcc aacacctaat gctactgaca tgaaagtcat agcagccaca gtaatacctg
     7081 taacataaat aatgagatac ttatttctac gcctcagcat gttatcggaa agtttgccgg
     7141 caagtccggg aacggcaaag aggatcagcc actgggcata aaaaaggaat tcacccacag
     7201 tattcaactc aaatgcatcc agcaatttag gctggtaatt atgataggca atccaactga
     7261 accctacaac gaaatttaat acgacgagac tggtaaatgc tgattggggg tattttttgc
     7321 ccatgactag tttggtttgc ctgcaaattt aataatgttg ttgtgtcatt gtttgaataa
     7381 tgaaaaaaat ttaggagatc tacatttcaa ggtatttctt tagatcctga gaaaagccgg
     7441 tcatgccatc actgtcgaat tctatttcaa aacgctccag ttaagaaaaa gaatagagcc
     7501 atggatgagc agtttcttca tcgcggccaa aaataaaatg agaaatggtc tgtcggggga
     7561 cagaccatgg atgataggat gataattttt ttacgcaaat ttaataatgt tgttgagtca
     7621 tagtttgaat aatgaaaaaa tttcagggtc taatttcaag gtattttaaa ctaagacact
     7681 acaactatcc attcaagagc ttcactttat tacctccacc cagcccgtac attccgcgta
     7741 tccgtccatc accaccacga agtaatacac gcccgcagga ctgttttcag ctttccaatt
     7801 attttggtag ttattcgttt caaatactac cttgccccag cgattaatca cagtcaaact
     7861 agcctggaga ccaatagcat ttgcggtttc agaaccatcg ccaaattgca ccagcagata
     7921 atcattcttt ccgtcttcaa ttccgggagt gattacattg ggtaccgaga gggtaaatat
     7981 gggtacctga attattttct catacgaaca tagtttgcct tttccaattg ctttcacagt
     8041 ataaaaccca tcttgatcat aagaatgagt aacctgatcc aaatcagaag tttgtccgtc
     8101 cccaaagtca aaataaacgg tatctcccac cgaataaacc gatttatcta ttaattcaat
     8161 caccggccgt tcaaagcaat tgttcttcat aatcaaatca aaatcgggaa taattggctc
     8221 caatacttcg atggtcaccg tatcggaata aaagcatccg gtgctttcga ggatgtctac
     8281 ataaaattga ccggtttgtg tcagcattac aacgggattg gcctggttag aaaatattgc
     8341 gccggtttga tcagaccagg aatatgcaat accgccggaa gccatcaact gatactcggt
     8401 attgtaacag atttcatcat cctcctggac ttgcgaatct cttccgtaga tattaattat
     8461 tttggttact gaatccactc caatgcaagt gttttgatcg acagacttca actgcacttt
     8521 atacgatccg ggttgttgat actgatgtgc tatggctgtt gtgtcagtct taaaaatttg
     8581 ggtgccatca cccagatccc attcaaaaat ttcgcctccg atacttaagt tctcaaatac
     8641 aatggcgttg gggtagcaca atgcacttac atttggcgat ttaaagtcga gggtattagt
     8701 acgaaactgt gcttttagta atgaaagatc gaatttgaat gccgcgttat tgcaattgat
     8761 gctattgttg gttgcggaat atgcaccggg cgtggtaggg aagtcagatg atgccgtgaa
     8821 acatccggaa caaacggcgt ggtaaacaat tccacgctta tcaaacctgc tggtgccacc
     8881 atccacatgc accgaagagg aggttccgcc caggtaggtg ccgtacaaga actgggtcgc
     8941 ttcctcattg agcaccatga aataaaaatc tgaaccctgg gtattctttt gaaaggcatc
     9001 agcggaaatt ggcaatccgc gggtgtcact atcccatcca aagcgcgaag aatttactaa
     9061 accgccccaa cctgcaagaa agagattgtt gcaatcgttt accagaaatg cggtgggaga
     9121 gatgtttgga atttgtgtgc ctgacccaac gacggtagac catattaagg ttgacaaatc
     9181 agccgaaagt ttttgcacaa actggccgct gtttggattg ctataccggc cgggagtaac
     9241 gggtatgttg ccggtagtct gcccgaaaag ataaacttca tcatcttcat taagatctat
     9301 gatatacacc tggtcaaaat tggcactgcc ggtataggta gagtttagga tgtttagccc
     9361 atcagaggaa atgacgctaa tccagccgtc agcagaacct ccataagacg actggtatgc
     9421 tccggcactt actgggaaat ctgtactcgt ggttcctccg gcaaccacaa tatttccctc
     9481 ctgatttatt ttaatagtat gggaggcgtc gtagccggaa ccacccaaat aaccggccca
     9541 cacaacagta gaaaggtccg gtgaaatctt cgccacgatg gcgtcggtgc cacctccgtg
     9601 aaaagaagtg gagaaactat taaccactgg aaagttaggc gaccaggtta ccgaagaaat
     9661 gtaaatattt cctgcatcat cggtgataat gtcaccccgg ttttcatcgc cgtaattttt
     9721 cgtcaacggt ccgccggtgt tgttgagtcc atcattgccg ccaccgccaa ggtatgttga
     9781 cttaatcagc gttgaaccgt ctttgctaat tttggctatg aagatgtcag aaccattatt
     9841 gtaattgatt acatcgttgc caaagacaga tcctccgcta taattctgac ttattacacc
     9901 aaatgttgta ggaaagtcaa tggagcctgt agttccaaaa acaataagat tttcatcctt
     9961 atccataatt agtgagtgtg gactgtcatt gtcgctaccg cccaggtaac ttgcatacaa
    10021 aagattttgc cccagtgaat cgtactttaa aatggataca tcgtatattc ctccataagt
    10081 ggtctgaaaa gcaccgggtg tggccgggaa tgttccgctg aaagtacttc caacgaaatg
    10141 atttgtaact ccggaggagt ataatgttcc tttctcgcca ggagtagcag aactacccca
    10201 attgtccgcc atcgaaccag agtaagttga aaagatgagg agcggatcaa ttataagtcc
    10261 cattgagtca tcataacctt caggaaagtc gaactttact ttttgatttt caatttggta
    10321 taaaaccgga acattaattt tctgttcacc ccttatttgg aaagcaatag gtttcatttc
    10381 tacaactcta ccgagggaag tctgaatcat aaggtcacca ttttccaatc cgattaaatc
    10441 agagcctttg tactcaataa taatttggtt gggatctccg cagggttcaa taatgaaatc
    10501 atattttaga ttcttattga ctgatgaaat ttgcaaatca attccttcat aaatttcaga
    10561 atataataaa gtctcatagg caaacgcttt actgacccaa ttggacttat ccttacccag
    10621 aaaaaaatta tagtaagcgg acttcttttg tttggcaatg aatttggaat ggggatttga
    10681 acctgcaaat gagagctgca aattgtgtcc ctgaatgttg tcattgcaaa catacctttc
    10741 atctgattct gagattaatc cgtgcgacat cagatgataa ttcccgatgg cttccgcatc
    10801 aaacaaggta atgatgaatc cggaagaatt aacaagcagt tttccaccgg aaaaccaact
    10861 gccaaagtca tagttggtat cccattgacc cttgttttca ataaaactaa gtgatgaatt
    10921 attgctttga ctataccccg gaaatggaac aagaagtagt attgtgataa ctatcttttg
    10981 gtaaaacatt tggaatcaga ataagctcaa atctacaaac aatgcaccat ttgtgggttg
    11041 taagaaattg cattgtcatc caaattttat tcattatccc ccaattaatg ggattttgga
    11101 ttattcaaac cagataaata ggatttgata cacttcgcca accataccga ggctggccag
    11161 acaaatgccc aatgcgccaa tagctttacc attagagaat ttattgagag agttgatgaa
    11221 atcaacagtg agatgcagtc caagaattat cccgaaaatc cctcctacaa ttccaaatag
    11281 cagccacaaa taccagggca aggtaaataa cccccaaatg gtgggtacga ctgcagcaaa
    11341 taataagatt ataaagagtg taataagagt ttgaccggcg tgactgcggg taactttgta
    11401 agaggctcgc tctgtaaatt tctgaatgcc acggatggct ttttcaattt tatcagcggt
    11461 acggggtgat ttaatttcga taaaggcaag ggtaatgccg gctacttcaa gtatgagtgt
    11521 gatatactga agggaattgg agacagaatg atcaaagaac ttcatggcga ttgtatgttt
    11581 gcaaaaaaaa aatattttaa gacttcgcta actccatgca ggcttttacc agcacgtcca
    11641 gttcttttgg agtagtatac acatttggtg agatcctgca gccatgaact ccggcaccat
    11701 ctattgccac tgtatatatc ttatattttt ccatcagggt atctgcaaaa tcttttggtt
    11761 tcattccttc aacacccaca ttgccaatgc cgcaatacct tgcaggatcc ctgggagtat
    11821 ttacaaccac tcccgggata tcccggactt tgctggtcca gtaagtttgc aaataccgca
    11881 atcgttcttc tttacgcttc ggtccaatca tattatgaaa gtcgatggca tttgcaactg
    11941 ccagatccgt atgaacggga tgagtaccta tatgattcag ccggctgatg tcatccggtt
    12001 ctctttctcc ctcggcaaga agaggccaaa cctttggtat agcagatttc ttagcataca
    12061 aaattcccgc accgagcggc acactcagcc atttatgaag acttgaccca taaaaatcgc
    12121 aatccagatc tttcattgaa aaagtaaagt gaccaaaagc atgagcgccg tcaaccatta
    12181 cactcactcc tttgctatga gccatgtcac aaatttttct tacaggaaga atatgtccgg
    12241 taatgtttat gatgtgacaa accatcagaa gttttgtttt tgatgtaatg acagattcat
    12301 ataactgaac aatttcctca tctgatttgg gatggttggg taccgaaaca attttgttta
    12361 caacgccgta tcgtttggcc acttgcttaa acatgttcag catggctcca taatcctgtt
    12421 cagccattac cgcttcatct ccggcgttcc agtcaagacc gccaattacc atatcaaggg
    12481 attccgtagt attccgggtg aggatgagct catcgggttc acaattatgg agcgtggcaa
    12541 gcttggtcgc catggcattt ttattatccc actgcacggt acgcatataa taggaaccct
    12601 gataatttac ttcgcgaaca tgcctgatga aattttcgag agtttcctcc ggaagaaagc
    12661 aataatagcc attctccagg ttgatataat caggtctaag tttgtaggca cttctgattt
    12721 ttgcccaaaa atcttcatcc gaagccaatt tttctgccgg aatgtgactt acactttcgg
    12781 taagattggc cagcactttt agagatgaag ttgcaccaaa gcccagcaaa gcaagacttt
    12841 tgatgaaaga tcgcttatcc atatgtgttg gttctgatta agaaaagaaa aggtaaggaa
    12901 cctgtttaat ttttgcaact tttagttact aaccggtaat agtcattttg acaattctga
    12961 ttttagttgg tattaaaata aaaaatgaaa ggattacttc ttgcggaaaa ctatttcccc
    13021 atttactata gtagcataaa cttcaccact taataatgcc gaaggatccg aatttccagt
    13081 ttgaaaagga tcaattgtaa gcacagagat atcagcccat tttccaggag ctaaaattcc
    13141 ggtgctgtct tcccggaata cagaccaggc agcccaggtt gtgaaagcac gcaaagcttc
    13201 ttcgggtctg agcgcttctt ccggatgcca gccaccttca ggttcaaagg atttattttg
    13261 ccgggtgatt gatgaatgca gtccatagaa aatattgtga tcggttccgg ttagatcgga
    13321 attgaaaatg atatgtgttc cctgcttcct caatgtgcgc caggcatacg ctcccgttat
    13381 gcgttcatgt ccgactctat cctctgccca caccatatct tccaccgcgt gggaaggctg
    13441 catggatgca atgatattta gctccgccaa tctttgaaaa tcttccgcat gcagcacttg
    13501 tgcatgttca atccggtttc tcaagttttt tatttcagga tttgcttcga ttaacacctc
    13561 caggaaatca agagtttgtc tgtttcccgc atcaccaatc gcatggatac acagctggaa
    13621 tcctgccgta ctcatttctg ccaggatatc cggtttaaac cggtaatcca aaccgctggt
    13681 tcctttgtga ccgggcatgt cagaataatc ttccagcaat ttggctcccc ttgaacccag
    13741 ggcgccgtca tagaaagctt tcactgaatt aattatcagc attcctgaag ctgattcgta
    13801 aggccctcgt tgtttccatt cactgagtaa atcttcgtca tctccactca acatcgcata
    13861 aacccgtata ggtagtttcc cctctttatc cagttccata atcgcggtga gtaattttct
    13921 attcaccccg gcttcatgaa tggccacata gccggattca gccatttcga ttaggcccgc
    13981 aagaaaatat tcttttagct gctgattagc aggttgagga attgcagctt caataatatc
    14041 cgaacctttg tttaggaata gcccggtggg ttcgttgttt tcatctttca ccacttctcc
    14101 tcctggtggc gatggagtat cctttgtaat tccggcgttg tccaatgcca tttgatttgc
    14161 ccaggaagcg aagctgtgca aactaagcat cagcacagga tgcttcggca ccatttcgct
    14221 cagcattttc ttgtccggat attgggatgc ccaggcacct tcatcccatc ccttgccaat
    14281 gatccattca cccgcgggaa tatttttaga cgtttcaaca attagggcta cggccgccgc
    14341 ttcggtttct actcccgtaa gatcaaccaa ttcgagattc tgtcccaggt ttataacatg
    14401 agtgtggctg tctaccagtc ctggaattat tgtgcctccc tcagcatcga tgatctccgt
    14461 gttctctccg gcaagttttt ggatttctgc agtagttcca atgttagaaa taagtttacc
    14521 cgtaatggcg atcgcttgcg cttcaggatg ccaggccccg ttggcaaaag gtgcgcccgg
    14581 agacgggttt ccatccaggt cagggtcagg ccattggaat gtgtaaactt tgccattgat
    14641 aatgatgagc tcagcgctgg gttctttgaa gcaggagctt agcgtaagaa agggaataag
    14701 aagtaaacat gggaattttt tcatggtgat gatgtctggc aatgaaaatt acaaaatttt
    14761 ccggtacaca aatatattct ccgggggatt tagatttccc gctgaccttc cggtgatatg
    14821 ttcatccgcg gatattcttt tctgatttcg gacaccaaaa tactccggaa aagagaattg
    14881 tggcctggca tacttattgg gttgctaatt caaaccattt cattaaaaga atatattgaa
    14941 aattgtttct gactgtaatc gaactgtaac ccaataaatc tgatgagatc aataagaatt
    15001 gcactccgga atatttatcg ccttgataat atttcagcgc tgaatttaac gggactttcc
    15061 gttggaattt tttcagtcat cgtattagga ttatttgtat ggactgaatt aagccatgat
    15121 acatttcttc ctgattataa aaaaatatac cgcatcacta ctcattatgt attaaaaggg
    15181 aatgaagcta actgggcatt gaatcatggg attatggata gtgaattgga agacaaatta
    15241 ccgaacttgg atcactcaac tgctttcata aaagtccagt cagaaaaggt attttactcg
    15301 ggagatttgt cattcaccaa tagtaatgga ttttatgttg atgaagattt ttttgaagta
    15361 tttaatttca attttttgta tggtgacctg tactctgcga ttcgtcaacc caattcaatc
    15421 gtaatcactc aatcactggc aaaagctttt ttcggtaagg aagatgctgt tggcagggag
    15481 atagaaattc aagagattga tggaaagact ccactcgtaa taaccggggt tgttaaagat
    15541 ttcccagcta attcttatat aaaatttgat ttcgttattt caggaaaaag ttatcaacca
    15601 tgggatctct attatagcgg aaagagcgga aatatgttct acgttttctt caaaacactg
    15661 aaccaaattg acgctgaaga attacagcaa tcggtaaatg cagcattggg gcccattaag
    15721 aaggaatttt acggagctga gtatgaacct tccgaatctt tccggctgga caatcctgtt
    15781 caacgcattg acaaaattta cttcaatgcc aaaaatgaat ttgaaatttc tgccggtggt
    15841 aaccaaaatt ataccattct ggtgggtata gttggggttt tcatcttggc catcagcgta
    15901 ataaactata taaatcttac tatctcctct tcgttttcta agcggaaaat aaaagaattt
    15961 gccgtccgga aacttttagg ttcttcagcc ggaaatttgc tggcaggatt cgcactggaa
    16021 atttccattt gggtgtttat ggcaattgtc ggttcattga taatcctgaa attcattttt
    16081 gatctttttc cagctgccac tgacttaatc tcgagtcctt tcagttaccc tggattttat
    16141 ctgataatca taatttgctg gattctctta agtctgatta cctcagtatt tcctgcttcc
    16201 aaattcctga acttcaacat tgttcaatct gccaaaggaa atatccaggt tggtggatca
    16261 aggacattga atatgcggaa tttactggtt gttttccagg taattctaac tgcaattctt
    16321 atttccaact ccactttgtt atttcgtcag cttaattttc ttaccacgaa aaatgccgga
    16381 ttcaaaactt cccaaataat tgaggtgaaa aggtcatcaa atatgacaga aggtgaatgg
    16441 agtaatttta tgaataaaac gaaatctctt tcattcatta aaggcgttgg cggttcacaa
    16501 tttacccata ttagtgatat gagtaacgct gaaataagga ccacagtagc taattcaccc
    16561 tttgtcaaag ttgcctggaa cagtgtcagc catgatttca ttccaacctt ggaaatacag
    16621 attgcacggg gacgaaactt ctccccggat cttagatctg actacaaggc tactgttata
    16681 aatgaaaccg ccgccagggc attgaatatt gatgaacccg aagggaaatt catcataatg
    16741 tggggcgaca ccctgcaggt agtcggaatt gtggatgatt ttcattttca aagttttagt
    16801 aaaccaatta gtccgttaat ttttctgttg cgacctgaag ggttaacgta tgtagcgtac
    16861 attaaattcg aaaatgatga taccgctgat ttaattggtg tactcaacga tcattgggat
    16921 tctgaaaacc tttatgcgct atttgattac aggctaatgc aggataattt caaatctcta
    16981 ttggagaaag aggaacaaat ggcgaacata gtcgcatcat tcaccattgt ggcaattatt
    17041 ctggctatac ttggtttaat aggtctggtc agtcatgatg caggccggaa atccaaagaa
    17101 atagctatca aaaaaattct gggggcacct atcctgcaca taattttatt aataaaccga
    17161 agatttgcat ttatactaat tgcaggattt acaatatctc ttccattagc catttggatc
    17221 ggaaattatt ggcttgaggt ttttgtttac cggatatcaa tttctgtgtg ggatgtgctg
    17281 gtttcggtaa taagtatcgc tttgcttgga gttttaacct ccagtttgat tacttataat
    17341 gcggcatcat ccaatccagt gaacgtaatt agaaacgaat aaataatttg catattcatt
    17401 tctgattgcc ttcaatccgg aattacaatt ccccttcctg aaaaaatatc tcagtactga
    17461 ttttttatta ccgaaattca aaaaatctga gtaagttgta agtaagaata aatcaggatg
    17521 aacgaatact taagccagga aaatttgaat ttcattttgt ttgaagttct taattgctca
    17581 ggtctcaccc aggcggaata ttttaaggat tataatgaag aatctctgaa gctattactg
    17641 aattccataa aggacttcgc cgacagagac ctctttccaa gttttcgggg aatggataat
    17701 caacctgcgt attttgagaa tgggaaaatt gttattcatc ctgcctttgg aaacataatg
    17761 aagaaaatcg ctgcactggg attgatgggc agcactttta agtctgaaga tggtgggtta
    17821 cagttaccat caacaattta taccgcggca tatttcatca tgggtgccgc taataataat
    17881 gtgcccggat attcaggcct caccagcgga gctgctaacc tcattaatac cttcggaagt
    17941 ttaacgttga aagagaaatt tgtttccaac atgctgtccg gtaaatgggc tggaacgatg
    18001 tgtatgacag aaccccaggc ggggagcagt ttgtcggata ttgccacact ggcaactcct
    18061 gctgatgagg gttcatacaa aattaaaggg cagaaaattt ttatctcagg tggcgatcat
    18121 caattctgcg agaactttat tcacctgacc ctggccagaa ttgaggatgc gcctgctgga
    18181 acaagaggaa tttcactatt tgtggtgcct aaattcaggg aagaaaataa tgaagttgca
    18241 gataatgatg ttatagtagc gggggaattt caaaaactcg gacaaaaagg atactgcaca
    18301 acacatttaa tttttggtga taataataat tgcaggggct ggctggtagg cgagcccaac
    18361 atggg
//
LOCUS       DASWHX010000122         3691 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_1598198, whole genome shotgun sequence.
ACCESSION   DASWHX010000122 DASWHX010000000
VERSION     DASWHX010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 3691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3691
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1598198"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..341
                     /locus_tag="VGA21_08530"
     CDS             <1..341
                     /locus_tag="VGA21_08530"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9650592.1"
                     /translation="AYDQGDYEQAALLFEKVITTSDNRLLDMFYLGNAYLAIGNAENA
                     IKNFKAVIKSETGLTTVAKWYLGLCYLKAEQTDLAREMLLEVQNSGDERAEKAAVILR
                     KLIKNDFNKN"
     gene            382..738
                     /locus_tag="VGA21_08535"
     CDS             382..738
                     /locus_tag="VGA21_08535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650593.1"
                     /translation="MHLGIVHDDGPSAMANGPQKRVGEIFVFDHDDKSDTKFIIYNFN
                     NGELGNIFEYLRGQLVNLKFDDKDDPIITEGKVTVTGIASTDSIGFKETEDGYKNWRN
                     EQALVLKPITGRRPVI"
     gene            complement(786..1640)
                     /locus_tag="VGA21_08540"
     CDS             complement(786..1640)
                     /locus_tag="VGA21_08540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650594.1"
                     /translation="MRILIWDMEGEKWDFVVVSVPGRPGPGPSDEYLKVIGPEVQPDG
                     NGNFTSTAYSEEEINAVHAFAVARLTIHLWEKAVGLKIIWQWNAGKRKNERPLNIQID
                     DTQSAGFHLVRESAVYGLYKEKRTCKSLDIVSHEITHGIIESLRPDIHYINTVQAYAV
                     VEALADLSAILLHASIPELYLKSADKSNSDLRLKNPVSEFAEGYGKAGEGIRSALDKV
                     STANDSYSLAKPLVNSVYMKLVEKWEKSPSGIVGLLAWVEELTHYAFYPLLNLREISP
                     GNYFSLLK"
     gene            complement(1661..>3691)
                     /locus_tag="VGA21_08545"
     CDS             complement(1661..>3691)
                     /locus_tag="VGA21_08545"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HEX9650595.1"
                     /translation="AENYIQYSHIYRLMNQPDSMKYYAGASLKIYQKLYKEGNLATAA
                     CHQFIAIANKMESKYTEALTNIQQALIDSDKSFNSLNISANPTLNQATNLDKLYELLA
                     IKANLFYLRYQESKNSSDLESAYQLYVTLDELTDEIRAGLYSRTTKLLINAALKREAE
                     NALKCIYFMFTTSNSKEYLGLAYKYLEKNRYAEMFENLSSVQAVSQTGLPDSLYRLEL
                     DLQFRLANQKQLLLSTNDPASIDSIKEMMFSINKENQELQDYLSQNYSSYYQSKYGEL
                     ISLDSLQKILVDKTQLVEYFIGKSSLVIFSVTKESSAIILVPLSEEFTYNINSLLAQI
                     SAPINLMIPLDQLYQTYSNSAFSLYKILIEPVVETRTTKLIISPDGILSKLPFECLLS
                     NDAKADFKSAPYIFKNMDIQYVYNLNLLYKFTKPKLQKTPSLLAFSYSDIDPSKVALR
                     SGRSEIPASALELIAISRFFDGDNDNFLIGEEATETAFKNLSSRFEIIHLAVHGVGDT
                     TKAINSRLEFKVGKDTLNDGKLFAHELYNLNLERLRLAILSACETGIGKELPGEGVFS
                     IARGFAYAGCPAVVMSLWQVNDTYTAKLMEYFYANLTQGKEIDLAVRNAKLTFLENAD
                     SREAFPYYWSGFIAQGNVSPVTRHRFRYIYLLYFLLPLALAGGFIYVRRRRK"
BASE COUNT         1116 a          724 c          745 g         1106 t
ORIGIN      
        1 cggcctatga ccagggggat tatgagcaag ctgcgctcct ttttgagaag gtcatcacca
       61 cgagtgataa ccggcttttg gatatgttct acctggggaa tgcgtacctc gcgattggaa
      121 atgcagagaa tgctataaaa aatttcaaag cggtaattaa aagtgaaaca gggttaacca
      181 ccgtagccaa gtggtacctg gggctttgtt atctgaaggc ggaacaaact gatcttgcca
      241 gggaaatgct gttggaagtg cagaattccg gagatgagcg tgcagagaaa gcagctgtta
      301 ttttaaggaa gcttattaaa aacgatttca ataaaaattg aaataattct aaacccatgt
      361 tcaatttaaa tatttacaac tatgcattta ggaattgtgc acgatgatgg accatcagca
      421 atggcaaatg ggccacaaaa aagggtaggt gagatttttg tttttgatca cgatgataaa
      481 agtgatacca agttcattat ttataatttt aataatggag aactcggaaa tatttttgaa
      541 tacctgcgag gtcaacttgt taatttgaag ttcgatgata aggatgatcc cataattact
      601 gaaggaaaag taacagtaac aggaattgca agtacggata gtattgggtt caaagaaaca
      661 gaggatgggt ataaaaactg gagaaacgaa caagcgctgg ttttgaagcc aattacaggt
      721 cgaagacctg taatttaaat acatattagc tatagtagca ttgggaaaac aataaactca
      781 taaatttatt ttaagagaga aaaataattt ccgggagaaa tctccctcag gttcaataat
      841 gggtaaaacg cgtaatgtgt tagttcttct acccaagcca agagtccaac tatacctgag
      901 ggggattttt cccacttctc aaccagcttc atgtaaacgg aattcaccaa tggttttgcc
      961 agtgaatagg aatcattggc agtagaaact ttatcaagag cgcttctgat gccctcccct
     1021 gctttcccat atccttcagc aaattccgat accggatttt ttagcctcag atcagaatta
     1081 gatttatccg cggatttcaa ataaagttcc ggaattgatg cgtgcaataa tattgctgat
     1141 aaatctgcca gggcttcaac caccgcgtag gcttgcactg tatttatgta atggatatcc
     1201 ggtctcagac tttcaataat gccgtgcgta atttcatgag aaacgatatc aagagatttg
     1261 caggttcttt tctccttata caagccatag acggcggatt ctcttaccag gtgaaagccg
     1321 gcactttgtg tatcatcaat ttggatattc aggggtcttt cattttttct ctttccagca
     1381 ttccattgcc agataatttt caggcccaca gctttctccc ataaatgaat ggttaacctg
     1441 gcaacagcaa atgcatgtac tgcatttatt tcctcttcgg aataagcggt acttgtaaaa
     1501 ttgccattgc catccggttg aacctccggg ccgataactt tcaaatattc atcagacggt
     1561 ccgggtcccg gtctcccggg aacactaaca accacaaaat cccacttttc accttccata
     1621 tcccagatca ggatgcgcat tttgcagtat tgctgattcc tcattttctt cggcgtctaa
     1681 cataaataaa gcctcctgcc agggccagcg gtagaagaaa atacaataga taaatgtacc
     1741 tgaagcgatg tctggttacc ggactcacat tgccttgcgc tataaatcca gaccaatagt
     1801 atgggaatgc ttccctgctg tccgcatttt ccaggaaagt tagtttagca ttgcgcaccg
     1861 ccagatctat ttccttgcct tgagtaagat tagcataaaa atactccatt agttttgccg
     1921 tgtaggtatc attaacctgc cataaactca tcaccaccgc cggacatccg gcataagcaa
     1981 agccgcgggc aatactgaag acgccttccc ccggaagttc cttccctatg ccggtttcac
     2041 aagcacttaa aatcgccagt ctcaatcttt ccaggttcag attatataat tcatgagcga
     2101 ataatttgcc atcattcagc gtatctttac ccactttaaa ttccagtcgg gagttgatag
     2161 ccttggtggt atcgccaaca ccatgtacgg ctaaatgaat tatttcgaac ctggaggata
     2221 aatttttaaa tgcagtttcg gtagcttcct cccctatcag aaaattatca ttatcaccat
     2281 cgaagaatct gctgatggca atcaactcaa gtgcagaagc cggaatttcg ctccttccac
     2341 ttcgtaatgc caccttggag gggtcaatat ccgaataact gaaggcaagc aacgaaggag
     2401 ttttttgcag tttcggtttg gtaaacttgt aaagtaaatt cagattatat acatattgaa
     2461 tatccatatt tttgaaaata tatggagcgg acttaaaatc agcctttgca tcattactta
     2521 aaaggcattc aaaaggaagc tttgataaaa tgccatccgg ggagatgatt agttttgtag
     2581 tgcgggtttc tactacaggt tcaatcaata ttttgtataa ggaaaatgcg ctgttgctgt
     2641 aagtttgata tagctgatcc aatggaatca ttaaattaat tggagcggaa atttgggcca
     2701 gaagcgaatt gatattataa gtaaattctt ccgataacgg gactaaaatt attgctgagc
     2761 tttcttttgt aactgaaaaa atgaccaaag aagacttacc gataaaatat tccacaagct
     2821 gggtcttgtc aaccaatatt ttttgcaaag agtctaaact aattagttcc ccatatttac
     2881 tttgataata agaggaatag ttttggctca gataatcctg taattcctgg ttttccttat
     2941 taatactaaa catcatctcc tttatagaat cgatggatgc tggatcattt gtcgataaca
     3001 acaactgttt ttgattggca agcctgaatt gtaaatccag ctccagacga tataggctgt
     3061 ctggtaaacc ggtttgcgat acggcctgaa cagaggaaag attttcgaac atctccgcat
     3121 acctgttctt ttccaggtat ttgtaagcaa gacctaaata ctccttacta tttgaagtgg
     3181 tgaacatgaa gtaaatacat tttaatgcat tttccgcttc ccttttcaaa gctgcattaa
     3241 ttaaaagttt ggttgtccgg gaataaagac ccgccctgat ttcatccgtt aattcatcaa
     3301 gggtaacata caactgatat gcagactcca gatcagaaga attttttgat tcctgatagc
     3361 gcaggtaaaa taagtttgct tttatggcca ggagttcata aagcttgtca agatttgtag
     3421 cctggttcag agttggattg gcagaaatat taagcgaatt gaaagattta tctgaatcaa
     3481 tgagagcttg ctgaatatta gttaacgcct ccgtgtattt agattccatt ttgttggcga
     3541 tggcgatgaa ttgatgacac gccgcggttg ccaggtttcc ttccttgtat agtttttgat
     3601 aaattttcaa agaggctccg gcataatact tcatagaatc cggttgattc atcaaccggt
     3661 agatatggct gtattgaata taattttcag c
//
LOCUS       DASWHX010000123        22750 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_3420465, whole genome shotgun sequence.
ACCESSION   DASWHX010000123 DASWHX010000000
VERSION     DASWHX010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 22750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22750
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3420465"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1692
                     /locus_tag="VGA21_08550"
     CDS             <1..1692
                     /locus_tag="VGA21_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012927801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX9650596.1"
                     /translation="EADYPVISPKSDMIVFLRNNQVWSASLLDTESKPESLFQTRGRV
                     GSLEWSPDGSKLAFVAFRGDHSFIGVYTNSETPIQWISPSFNRDSNPGWSPDGSRLVF
                     VRRPGGGGTPDSILVRNIRPWEIWTADINSGEASLLWKSPNTPEGSTPTTHGGYNLRW
                     AANNRITFLSYMDGWPHLYSINSSSDNPILLTPGNFMAEHISLSPDGRWLVFSTNTGP
                     DKLDLDRRHAARVAVDKPGVEILVAGNGLEWAPVITGDGNTIAMISATAQRPPLPAVM
                     PANKGKLKLLSENLISNYPTNKLVTPTQITFQSEDGLTIHGQMFTPEGGPAKKPAIIY
                     VHGGPPRQMLLGWHYSDYYSNAYAYNQYLCSRGFIVITVNYRLGIGYGYKFHQADNAG
                     SAGASEYKDIKAAGEWLSRQSFVDASKIGIYGGSYGGYLTAMALGRDSQLFAAGVDIH
                     GVHDRTVNRTLNLIMPDSYERAPDANLASKIAWESSPVSDVKTWTSPVLIIHADDDRN
                     VQFSQSTDLVQRLHAKGVYVETLVVVDDTHHWMKFSNALKVGEETLAFFRKQLMK"
     gene            complement(1698..2288)
                     /locus_tag="VGA21_08555"
     CDS             complement(1698..2288)
                     /locus_tag="VGA21_08555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEX9650597.1"
                     /translation="MGYYLILLGGILWLIPNPAEKERWLVQRSSNLTIEGKSNISDFS
                     CEIEGTNITDTLTINYDDDGGQITFVRKQIKINTVSADCGNNLITKDFRETLKSDKHP
                     DIFLNFLTLERPQLHDFSALKVNGTIEIIIAAVARKYEVTYQIIPHTTGKIELIGSQK
                     MRLGDFKLQPPSRLLGLLKVEEEITVDFHLQLTPIL"
     gene            complement(2377..3675)
                     /locus_tag="VGA21_08560"
     CDS             complement(2377..3675)
                     /locus_tag="VGA21_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018479537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650598.1"
                     /translation="MKAITQKLLLTLVLIAFGGALTLAQERALQYWNPNDLRSLNEFE
                     TTKEDTVGFTGLKVLVGGAFAQQYQNIHHSNEAVAVFDASGTNLNQLKIIGGGFNLAT
                     ANLNIDAQLTDGIRLNLVTYLSSRNHQEAWVKGGYVQIDKMPFFKSELIDKIMNSVTI
                     KMGHMEINYGDAHFRRTDNGNAFFNPFVGNYIMDAFTTEIAGEVYYHTPMGLFGMVGL
                     SGGEIKGDITNPDNRAPSYYFKGGWDKQLNDNMRFRLTGSLYTTKKSASNTLFGGDRA
                     GSRYYIVMENVNASTTPAFSGRWNPGMRSKITSFTVNPFVQIYGFDFFVNYEKAMGRA
                     SNESENREASQFGVEGVYRFLNNKLFAGIRYNTIQSELTGSLTEITINRIQASAGWYV
                     TRNLLLKGEYVNQQYKDFTNTSIFYNGEFDGFVIEAVVAF"
     gene            complement(3687..4265)
                     /locus_tag="VGA21_08565"
     CDS             complement(3687..4265)
                     /locus_tag="VGA21_08565"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEX9650599.1"
                     /translation="MKKVPFILLLLLAFGVRGQTILKLDGNSELSVSGTSTLHDWTIV
                     AEELSGKAKFEITDGTLLSVSELSIKVKTTEIKSGKSGMDNNTFKALKSDKHPYITFA
                     LSAAGATKSGSKYVIKSAGNLSVAGVTKPVVFTATCVLNANSVGCEGSYAMKMTDFDV
                     EPPTAMFGTIKTGNEITIDFNVKFENSELSKL"
     gene            4506..5084
                     /locus_tag="VGA21_08570"
     CDS             4506..5084
                     /locus_tag="VGA21_08570"
                     /inference="COORDINATES: protein motif:HMM:NF025013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4142 domain-containing protein"
                     /protein_id="HEX9650600.1"
                     /translation="MKIVFKSSFIYIVLSLLTVLFLSACVRNWTYSFTKDRNENKFRD
                     EGMVDEVVFVMEMKSAGILMRDISSYARDSAYSAMVQKFATELNSDYILFDPELTVLA
                     AKQGIKLSNHLNSEHWAMYRQVKGKKKADFDKEFLKVVHDVHTQLYDKSNQLAYQGKN
                     NAIRSFCAKKINFFQQNLAQSGKLSDEVVTIN"
     gene            5220..5714
                     /locus_tag="VGA21_08575"
     CDS             5220..5714
                     /locus_tag="VGA21_08575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650601.1"
                     /translation="MGTLLLIFFTLSNTLNNFSSQARIDPLLAQYLGNDLPSSLLEVE
                     NTPLTTVVLYDPFYQKELFGEIPREFLVNESVSGDHIQLVYEFNKIKAEANGYGPTLN
                     IIKEMSSIFGKPTEVQWLEKRINLSWDLPKLKLSLVHLELDSKGVIIVRVDLQNNQIL
                     VTSK"
     gene            6129..8774
                     /locus_tag="VGA21_08580"
     CDS             6129..8774
                     /locus_tag="VGA21_08580"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9650602.1"
                     /translation="MGSKRVLPPRLAIKCLRLFCSDQWVDQVLGDLLEQFEDDSKGKG
                     AMSARVLFWLNVLAFVRPHFIKKQKRDKQILMINNYIKTSYRNLIKSKVYSIINLLGL
                     SAGISSFLLISLYLNNELTYDSFFPDSDRIYRISLERVYPDRTRLFASSPVTLAPVLK
                     DNYAEVESITRMHRVFFQNELMVTVGEDVFNETKFYFADKDFFKVFPYKFLYGSPEEA
                     LSDPANVVLTRSIAIKYFGEENVLGRTINQGGELIVTGVIEDLPKNTHIHFELLGSIH
                     ALGYLQNAIDTNAWINPWIYTYVKLKPGTGPENFQSKFDDLVRTFGAASISQQMGPDY
                     ESEGHKFNYFLQPIQDIHLHSNLDIEVEPNSNIQYVYLLSAIAIVILLISSINFINLS
                     TARSSERAKEVGIRKVLGSYKSNLVYQFITESVMMSMAGSLLALAIVFYVLPSFNFIL
                     KKSLTMGFFLDPVIIAIFVVFIIFVGVVSGLYPALVISSLQPSSVLKGSFKASNKGTF
                     LRNSLIILQFAISIVMISGSIIVTQQMWFLKDKDLGFDKENTLVIRGFQALGNNVNAF
                     KNEIRQLEEVIAVGSSQGVPGDFLGSNIFSSDEEGITDLRANTVNIDDDFFATIGFEL
                     LEGRAFNAEFNDSLNIILNESAVKDLGLENPVGKRIRSAGPRDQNPDFTIIGVVKDYN
                     FYSLHSPITPLVIFNSSPNNNPPVMAIRIAGGDFQNTLNKLNKVWAEMATTGSIEFTF
                     LDQYLENQYEADRASGIVFDIFTLIAIIMSCTGLFGLATYIVQQRTKEMSIRKVHGAS
                     LLNIVSTFSREFVKLILIAFILGVPLAYFLMDQWLTNFAYHVNISIVTFLLAALFAVV
                     LVLITVSYQSIRVALLNPITSLRND"
     gene            8784..9104
                     /locus_tag="VGA21_08585"
     CDS             8784..9104
                     /locus_tag="VGA21_08585"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX9650603.1"
                     /translation="MNQFSLTSREEMILITVGVLQPDAYAYGIKKEIKSEAGASISLA
                     TIHTILYRMEKQKLLQSSMGGSSEKRGGRSKRLYKLTNKGFHTIKELQTARTSLLLKF
                     QSGN"
     gene            9537..10340
                     /locus_tag="VGA21_08590"
     CDS             9537..10340
                     /locus_tag="VGA21_08590"
                     /inference="COORDINATES: protein motif:HMM:NF025679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4382 domain-containing protein"
                     /protein_id="HEX9650604.1"
                     /translation="MMTYLRRFLLVVGTAIILFSCEDTGNTSRIMVTLTDSPGDYKEV
                     NVQVIGVEVHRQSGEQGSGWISLDMDGQTNINLLDLTNGATALLADAEIPSGQISQIR
                     LILGNENTVVIEGETEDVTYDLGTPSAMQSGLKLQVHETLLSGITYEFKLDFDAAKSV
                     VKAGTSGLYNLKPVIRVITEATSGAIKGSVLPAAENIAVYAMQGEDTIATSYAVENIS
                     EFLIGGLEPGTYNVVFDPGENSLLQTHTIEGVMVSIGVIIELNEVTLQE"
     gene            complement(10424..10774)
                     /locus_tag="VGA21_08595"
     CDS             complement(10424..10774)
                     /locus_tag="VGA21_08595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650605.1"
                     /translation="MKRLLLENQETTRMPKEAAELAMSMCQEKTSIQEGKFLLFWDNS
                     KEMSKTLKKLLTSLIRRSLRHYNPKLSRFAVLYTCSNFTFTSGKAEKDFGFQPKYSGE
                     KAIQDTIRHYSQKR"
     gene            complement(10795..11568)
                     /locus_tag="VGA21_08600"
     CDS             complement(10795..11568)
                     /locus_tag="VGA21_08600"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX9650606.1"
                     /translation="MRSTKDLATKWDRLAGNYEELVFNVFENDRNLLLIKSIRRLGSN
                     KKIAGDFGCGVGRALPLLSKHFKKVYAMDFSPMCVKEAVRLNLGNVICETKDLSNSHI
                     QLPAVDFGLCVNVAISNDVKKNHAILNNVCKALKRNGTAIFVIPSWESASMVTWRTME
                     LYNKDGFSVSEIPEEHLGFYTGKNIDVSMGVFDVDGSPTKHWLITELMQIFEKPGLHI
                     EAINKIEYDWKTELVRPPSWLGYPYPWDWMVKLRKVKHE"
     gene            complement(11704..12732)
                     /locus_tag="VGA21_08605"
     CDS             complement(11704..12732)
                     /locus_tag="VGA21_08605"
                     /inference="COORDINATES: protein motif:HMM:NF025100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
                     /protein_id="HEX9650607.1"
                     /translation="MYLHESVYISSQNTFPDMDLETLNPSFENKLTVKEPSYPGIPSS
                     ILRRMGKAVRYGVGCALPLLELKKDLHGIIIGTGHGGMEDCIKFLNQIIEYEEGTLTP
                     TNFVQSTPNAIASQLGMMSQNTGHNSTHVHSGLAFENAMLEAVMLLKEFPTHSYLLGG
                     VEEISTYQYNIERLGGWHKKEKCSNINLYDSNTEGSLAGEGAAMFLVNSIKENAVAKV
                     TEPLFFETADLIIVKARLKDFIKRYSLPPGTMLLTGENGDIRFNNFYQALEEELQGLP
                     IVRFKHFTGEYSSVTSAALWLCMQILTNQNVPAHMIKTGKIEKVDTILIYNCHKGIQH
                     SFTMVTKS"
     gene            complement(12735..13922)
                     /locus_tag="VGA21_08610"
     CDS             complement(12735..13922)
                     /locus_tag="VGA21_08610"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011583790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HEX9650608.1"
                     /translation="MKRVFVTGSGLVSSIGNSVEENLKSLRAGICGISKAEFLNTSYT
                     KSLLFGEIKIRNEEIISEFEINNEGVTRTALLALKAVSECINSSGLTKPELESETTAL
                     VGATTVGGMCLTDELYLDANSDHEASPFIDSYDAGAATRFLIAHFKIGGYVNTMNTAC
                     SSSANSIIFGSRLIKHGFARRAIVGGVDSLAKFTINGFNSLHILAAGQCMPFDNSRSG
                     LNLGEGAGFLVLESEEVVGSKPILGEITGYANTNDAFHPSSLSDEGKGPVLSMKHALQ
                     MAHLKKSDISYVNTHGTGTENNDLIESRAMIKVFETPPPFSSTKSNIGHTLGAAGSIE
                     AIYSLLNLQHQEVFPAINFKTPIQETGLIPVTKNEKLIINHVMSNSFGFGGNCTTLII
                     SKT"
     gene            complement(13929..14192)
                     /locus_tag="VGA21_08615"
     CDS             complement(13929..14192)
                     /locus_tag="VGA21_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011583791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HEX9650609.1"
                     /translation="MEKEALKQQLKEQIIEFLNLMDVKPEDIKDDQPIFGSGLGLDSI
                     DAIELIVLLERKYGIKINDPKEGRKILIDINTIVDYIEVNRTK"
     gene            complement(14198..15478)
                     /locus_tag="VGA21_08620"
     CDS             complement(14198..15478)
                     /locus_tag="VGA21_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011583792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9650610.1"
                     /translation="MVKLWASIRKDLLILTRDKLGLTLMFGMPIVLVLVITSIQNSTF
                     ELVNENRFTFLIVNHDNGELSDKLRESIKAMGSFRIIETYDETESGGILSLMDKNDAI
                     LALVIPETFTADLEKKSLKVTERVMFDFGVSDVPPSPVSDTVNSLKLYYTPVLQESFR
                     HSFQSAVLSALQLVENRHTLETIYQKISNKDLPEELENQLLHNKTNIEEIPASKDGAN
                     VVLNATQHNVPAWTIFAMFFIVISLGSGIVREKVSGSFIRLKTMPTSILVSILSKQIT
                     YLFVSIAQVIVIFSIGMYLFPVVGLPKLELPNDLVGLLIVSLICGLCALSYALCVGVF
                     SKTQEQANGFGAVSIFILAAIGGLLVPSFAMPESFRIFMILSPLYWCLESFHDLFLEG
                     GKLANMMPNLFPLIIITLFLQVLAFFGLKKKNLI"
     gene            complement(15478..16224)
                     /locus_tag="VGA21_08625"
     CDS             complement(15478..16224)
                     /locus_tag="VGA21_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011583793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9650611.1"
                     /translation="MSEKAVSVQNVNYRYPFQNKQCLEGINLDIIVGERFGLFGPNGA
                     GKTTLLSLMTGLFKLQTGSISLMEHPVGNRKEINALFGYVPQEFSFYEELTATENLDF
                     FGTLSGLRGQQLTSRITELMEILGLISSRKKRVQQFSGGMKRRVNLAIGVIHNPKILF
                     LDEPTVGVDIQTRHQIIEFLKNLNNEGTTLVYTSHLLREAEELCNKVALIDEGKIIEH
                     QTIEELLVSHNEVGLEGLFLKLTGKTYRDN"
     gene            complement(16224..17240)
                     /locus_tag="VGA21_08630"
     CDS             complement(16224..17240)
                     /locus_tag="VGA21_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013408266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtrH N-terminal domain-containing protein"
                     /protein_id="HEX9650612.1"
                     /translation="MKLDFTHIQAAHCENGVTTNLLQFAGIRELNEPLVFGIGSGLFF
                     IYIPFITVSNGPAFSFRTMPGHIFKRTCKALGIPVFRKKFNSKESAEKFLNEILRKNQ
                     PVGCQVGVYYLSYFPKEYRFHFNAHNLIVYGKEEDNYLISDPVMETVTRLSAFELERV
                     RFARGFLAPQGQLYYPVVNGNQITDTMISNAIRKGIKRNVRDMLHIPGPIVGVSGIRY
                     TAKRIRKWRDKLGVKDAGLYLAQLVRMQEEIGTGGGGFRFIYAAFLQEAHKFIPNDEL
                     LKISEEFTKAGDLWRSAAVQAAGIYKGRIGSQADFNQMGDYLEDISEVEKNAFLKLSK
                     VKLN"
     gene            complement(17286..17735)
                     /locus_tag="VGA21_08635"
     CDS             complement(17286..17735)
                     /locus_tag="VGA21_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650613.1"
                     /translation="MKKKDIPQDPSVLDNFTKEVSYVTDDDGKYTTGLSRGWEVKASA
                     LDVSWENIKSRVSEARDKVMQGKASPVLFFMELRIMDIPTLAAYTGYWRWTVKRHFKP
                     GPFRRMSVNRLKKYTEVFEISIDDLRNFETNPEIGETYLKGLEKSSK"
     gene            complement(17769..18908)
                     /locus_tag="VGA21_08640"
     CDS             complement(17769..18908)
                     /locus_tag="VGA21_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HEX9650614.1"
                     /translation="MTESVYITKTANFLPNNPVSNDEIEEYMGLINGRQSKSKRIVLR
                     NNGIQRRFYAMMKDGTPTHTNAEMTSLAIRKLFEKKSLSEIELLACGTSTPDQMMPSH
                     ACMVHGWLPESPSIEVVSPSGVCCSGMHALKYVYMSVKQGYANTAVATGSERLSRVLR
                     SEVFEEEMQRLESLEDNPYISFEKDFLRWMLSDGAAAFKIQNKPDTSGLNLRIDWLTG
                     ASYANEIEPCMYMAGEKQEGGILKSYMDYTPREMIEQSILSMKQDVKLLGEHIVEKGG
                     IKLKEACDKNGFDVKNVDYFLPHMSSEFFRMKIAEKLEENGMGIPQEKWFSNLTTLGN
                     VGAASIYLMIDELFNSGKLQKGNKILLLVPESSRFSYMYGLLTVC"
     gene            complement(18918..19817)
                     /locus_tag="VGA21_08645"
     CDS             complement(18918..19817)
                     /locus_tag="VGA21_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011583797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650615.1"
                     /translation="MSKRILAVYYSQSGQLKEILDNFLLPFEKSDHQIEILKVEPVND
                     FPFPWTSKKFFDAMPESVLVKPSELKPFELKSDKYELIIVAYQPWFLSPSIPVNSILQ
                     HPDFINLIENTAVITLIGARNMWLNSQEKVKKLITKEGGQLVGNIVLVDKHNNLASAV
                     SILHWMLGGKKDRYLGIFPVPGVSEKDIRDSSVFGQVALEHFNTDNLPGLQKEIISCG
                     GVEVKANLMFIEGRAGKLFSIWAKFISNRKNRTFWLIIFKYYLLFALFIVAPVVLLIN
                     NTLFKPFLGKSVQKKKEYYLGVG"
     gene            complement(19819..20313)
                     /locus_tag="VGA21_08650"
     CDS             complement(19819..20313)
                     /locus_tag="VGA21_08650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650616.1"
                     /translation="MLFRIAFAIIMGWSSISMAWQRDMQSSFNYDAYYQAFSSEDTLL
                     FNREIEALLPYSGIEKDAFYGAILMKKSKSLPTIKRKLDTFKEGRDLLENSIKSDTQN
                     LEFRFLRLATQENAPGFLNYDNNKKEDASIIVTSFGNLPPISRKYIREYARFSETLKP
                     EDLK"
     gene            complement(20554..21132)
                     /locus_tag="VGA21_08655"
     CDS             complement(20554..21132)
                     /locus_tag="VGA21_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009582724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEX9650617.1"
                     /translation="MRIKFTLLTLLVSFGILKAQSDWSIDKNHTNIKFTVTHMMVSEV
                     DGEFTDFAASVRNAGEDFSGADVEFTAKTTSVDTNNEGRDKDLRSDNFFDVEKYPDIT
                     FKGKITKEGEKYFLVGDFTMKDVTKQVKFDVRYNGKIDSGRGMKAGFKVIASINRFDY
                     NLKWNRAVEAGGLVVGEEVIITCNIELNEARG"
     gene            21351..>22750
                     /locus_tag="VGA21_08660"
     CDS             21351..>22750
                     /locus_tag="VGA21_08660"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008630455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEX9650618.1"
                     /translation="MAITGKPLAATAGAKIFHQGGNAVDAACAMLAATATMWDVLSWG
                     GETQALIYNPNTKKVIAINALGVAPTGATAEFYKDKGYEYPPQYGPLAAVTPGTPGGL
                     LTMLAEYGTMSLKEILEPAMQLAEGYPIEAQTANSIERQKERIKEWPYSKAVFLTHQG
                     EEREAPQPGEIFRQADLLATLQKMVDAEQQALKKGKKRKEAIYAAYDRFYEGDIADEF
                     VRGVQEQGGLITKQDLANWKVYIEEPVMTTYRGIEVYKLNHWVQSPVMLQSLNLLENF
                     DVKSMGYNSAKYIHTIYQVMNLAFADRDFYYGDPYFPPEEPIKGLLSKEYAKDRIKQI
                     NWEKNDPAVKPGDPYPFEGKKNPYLDLLEKWTNKISYSYPHRIIVPDGTNNADVTGFY
                     AGTTTVETADKEGWVVSITPSGGWVPAVIAGKTGVGLSQRAQSFVLTKEQNPFNVIEP
                     GKRPRATLTPTIALKDG"
BASE COUNT         6705 a         4647 c         4502 g         6896 t
ORIGIN      
        1 gaagccgatt atccggttat ttctccaaaa agtgacatga tagttttttt gcgaaacaat
       61 caggtgtggt cagcttctct gttggacacc gaatctaaac ctgaaagcct ctttcaaacc
      121 cggggcaggg taggttcact ggaatggtcc ccggatggat ccaaattggc atttgtggct
      181 ttccggggag atcactcatt tataggtgtt tatacgaact ctgaaactcc gattcaatgg
      241 atatcaccat catttaaccg cgacagtaat ccaggttggt cgcctgacgg atcaagactt
      301 gtatttgtgc ggcgaccggg tggtggcggt actcctgata gtattttagt gcggaatata
      361 cgcccatggg aaatatggac cgcggatatt aattccggag aagcgagctt attgtggaag
      421 tcaccaaaca ctccggaggg ttcaacacca acaactcatg gcggatataa cttacgctgg
      481 gcggcaaaca atagaataac atttttgtct tatatggatg gatggcctca tttatattct
      541 atcaactcct caagtgacaa tccgatattg ttaacaccgg gtaatttcat ggcagaacat
      601 ataagtttaa gtcctgatgg gagatggctg gtctttagca caaatacagg tcctgacaaa
      661 ttggatctgg acaggaggca tgcagccagg gttgccgttg acaagccggg agtagaaata
      721 ctggttgctg gaaatggatt ggaatgggct ccggtaatta ccggggatgg aaatactatt
      781 gccatgattt cagctacggc gcagagaccg cctcttccgg ctgttatgcc ggcaaataaa
      841 ggaaaattga aattgctgag tgaaaatctg atttcaaact atccaactaa taagctggta
      901 acgccaactc aaattacctt tcaatccgag gacggactga caattcatgg ccagatgttt
      961 actcctgaag gaggacccgc gaaaaaaccg gcaataatat atgtccatgg cggaccaccc
     1021 aggcaaatgc ttttgggctg gcattactca gactattact ccaatgctta tgcctacaac
     1081 caatatttat gcagccgggg atttattgtg attacagtaa actaccgttt aggaattggt
     1141 tatggataca aatttcacca ggcagacaat gcaggttccg cgggagcttc tgagtataag
     1201 gatataaagg ccgcgggaga gtggttatcc aggcagtcat ttgtggatgc atcaaaaata
     1261 ggaatttacg gcggttctta tggcggttat ttaactgcaa tggcgctggg tcgtgattcg
     1321 caattattcg cggccggggt ggacatccac ggagttcatg atcgtactgt taacagaaca
     1381 cttaacctga tcatgcctga ctcttatgag cgcgcaccag atgcaaacct ggcttcaaaa
     1441 attgcgtggg aatcttctcc ggtgagcgac gtgaaaacct ggacatctcc tgttcttatt
     1501 atacatgccg atgatgacag aaatgttcag ttcagccaaa gtactgattt agttcagaga
     1561 ttgcatgcaa aaggtgttta tgtagaaact cttgtcgtgg tggatgacac tcaccattgg
     1621 atgaaatttt ccaatgcgtt gaaagttggc gaagaaaccc tcgctttttt caggaagcaa
     1681 ttaatgaaat aactttgcta taagattgga gttaattgaa ggtgaaaatc tactgtaatt
     1741 tcttcttcaa cctttaacaa tcctaaaagc cggcttggtg gctggagttt aaaatctccg
     1801 agcctcattt tctgagatcc gattaactca attttgccgg tagtatgagg aattatctga
     1861 taagtaactt cgtattttct ggcgacagct gcaataataa tttcaatggt tccgtttact
     1921 ttcagggcag aaaaatcatg caattgcggt cgctcgaggg tgagaaaatt caggaaaatg
     1981 tccggatgtt tatctgattt aagggtttcc cggaaatctt tggtaatcag gttgtttccg
     2041 caatcggcgc tgacggtatt tatcttgatt tgctttcgga caaaagtaat ttgaccacca
     2101 tcatcgtcat aattaatcgt aagggtatcg gtaatgttag ttccttcaat ttcacaggag
     2161 aaatcgctaa tgttagattt cccttcgatg gtgagattac tggaccgttg aactaaccac
     2221 cgttcttttt cagctggatt gggaattaac cataaaatgc ctccgagcaa aatcaaataa
     2281 taacccataa caccacaaag ctaaacctca aataaataaa aatgccaggt tatacctggc
     2341 attttcaagt taaaaggaac ttaaaaagga acgcaactag aaagctacaa ccgcttcaat
     2401 gacaaaccca tcaaattctc cattatagaa gatacttgta ttggtaaagt ctttatattg
     2461 ttgattaaca tattctcctt tgagcaaaag gtttctggtt acgtaccatc cggctgatgc
     2521 ctgaattctg tttattgtaa tctcagttaa agaaccggta agttcagatt gtattgtatt
     2581 gtatctgatt ccggcaaaca atttattatt gaggaacctg taaactcctt ccacaccaaa
     2641 ttgtgaagcc tccctatttt ctgattcgtt agaggctctg cccatagctt tttcatagtt
     2701 aacaaagaaa tcaaatccat aaatctgaac aaacgggttc acagtaaagc tggtaatttt
     2761 acttctcatg cctggattcc agcgtccgga gaaagccgga gttgtagagg cgtttacatt
     2821 ttccataaca atgtaatacc ttgacccggc cctgtcaccg ccaaataaag tgttgcttgc
     2881 agattttttg gtggtataaa gtgaaccagt aagtctgaac ctcatgttat cattgagttg
     2941 cttatcccat cctcctttaa agtaataact tggcgccctg ttatccgggt tagtaatatc
     3001 acctttgatt tcaccgccgc taaggccaac cattccgaac aatcccattg gagtatgata
     3061 atatacttcc cctgcaattt cagttgtgaa tgcatccata atataatttc caacgaatgg
     3121 attgaaaaaa gcattgccat tgtccgttct tctaaaatga gcgtcgccat agtttatttc
     3181 catgtgcccc attttaatgg ttacgctgtt catgatcttg tcaatcaatt cagatttaaa
     3241 gaaaggcatt ttatctattt gaacataacc accctttacc caggcctcct gatggtttct
     3301 gcttgacaaa taggttacca ggttaagccg gattccatct gtcagctggg catcaatatt
     3361 cagatttgca gttgccaggt taaatcctcc acctattatt tttaattggt ttaagttagt
     3421 acctgaggca tcgaaaacag cgaccgcttc gttgctgtgg tgtatgtttt gatattgttg
     3481 ggcaaatgct ccacccacaa gcactttcaa tcctgtaaat ccaacggtat cttccttagt
     3541 tgtttcaaat tcattgagac ttcggaggtc gttcggattc caatattgga gggctcgttc
     3601 ctgtgcaagt gtgagtgcac ctccaaaggc aatcaaaacc agtgttaata agagtttttg
     3661 tgtaatagct ttcatggctt tttagtttat aatttagata attcagaatt ttcaaatttg
     3721 acattaaagt cgatggtaat ttcattaccg gttttgatgg tgccaaacat cgctgttggt
     3781 ggttccacat caaaatcagt cattttcatt gcataggaac cttcacatcc aacactgttg
     3841 gcattgagaa cacaggttgc agtgaaaact acgggttttg ttactccggc cacactgagg
     3901 tttcccgcac ttttaataac atacttactt cctgatttcg ttgcgccggc agcactaaga
     3961 gcaaaagtga tgtatggatg tttgtcggat ttcaatgctt tgaaagtgtt gttgtccatt
     4021 ccggattttc cgcttttgat ttcagtggtt ttcaccttta tgctcaattc actgacgctg
     4081 agcaaagttc cgtctgttat ttcgaactta gcctttccgg ataactcttc tgcgactatt
     4141 gtccaatcat gcagagtaga ggttccggag acacttagtt ccgagttacc atcgagttta
     4201 aggatggttt ggcctctgac cccgaaggcc agaagaagta gtaaaataaa gggcactttt
     4261 ttcattgtat tcctcttttt gttatggttc aaaagtattt tcggatgcaa caatttctaa
     4321 tgacaaaatc cagaacagat gatgattatt atcactctca tttatgtcaa aacgcataat
     4381 cattaacaca aaattgcaaa aaaattttga aaatgctaaa ggtgaaatct actaacgtat
     4441 tgattatcaa taaataagac gataaggaat gctttttgcg ctaattaaat aaaataaaca
     4501 aaatcatgaa aatagtattt aaatcaagtt tcatttatat cgtactttct ctcttgaccg
     4561 tacttttcct ttccgcatgc gtaagaaact ggacctacag ttttacgaaa gaccgaaatg
     4621 aaaacaaatt cagggatgaa ggaatggtgg acgaagttgt gtttgtaatg gaaatgaaaa
     4681 gtgcaggcat tctaatgcgt gatatttctt cttacgcgag agatagcgcc tattcggcaa
     4741 tggtacagaa atttgccacg gaattaaatt cggattatat tttatttgac ccggaattaa
     4801 ctgttttggc cgctaaacag ggaatcaaat tatccaatca tttgaacagt gaacactggg
     4861 caatgtaccg acaggtgaaa ggcaaaaaaa aggcggattt tgataaggaa ttcctgaagg
     4921 ttgtccatga tgttcatact caactttatg ataaatccaa tcaattagct tatcagggta
     4981 agaacaatgc cattcgttcc ttttgtgcaa aaaaaattaa tttctttcag caaaatcttg
     5041 ctcaatcagg taagttgagt gatgaagtgg tgaccataaa ctaatcctta aagacgttct
     5101 ataatgaaaa ttgaattttc attatgacaa aaattttctt tggaaattct ataattcaat
     5161 aaataagggg ctgaatgcgc tggcatgaat tttctacgta atgaggaaat tcaaaagcta
     5221 tgggaacgct acttttaatc ttctttactt tgtccaatac tttaaataat ttcagcagcc
     5281 aggcgcggat agatcctctg cttgctcagt acctgggaaa cgatttgcca tcaagcttgc
     5341 ttgaagtgga gaacacgccg ctaactaccg tagttcttta tgatccattt tatcaaaaag
     5401 aactcttcgg agaaataccc agggaattcc tggttaacga gtctgtatca ggtgaccaca
     5461 ttcagctggt ttatgagttc aacaaaatta aagccgaagc caatgggtat ggtccgactt
     5521 tgaatattat caaggaaatg tccagtattt ttggaaaacc aaccgaggta caatggttgg
     5581 aaaaaagaat aaatctaagc tgggatttgc ctaaactaaa attgtctctg gtgcatcttg
     5641 aattagactc taaaggagta attatagttc gcgtggattt acaaaacaac cagatattag
     5701 taaccagcaa gtagtgtcgg aaatgggtag tgtctcagtt tggaatcatt atacgtgtgc
     5761 ccactttttc gacacggaaa agcaatcaat ttcacctaat aaagactgaa gtgtggcaca
     5821 cgttagatta atttcattgt gatcttaaca gagacactac acggaaatgt aatcctgaat
     5881 tgaaccgatt agccgcattt tccttggaat cacggaacag gtggaatgcg gtccaccatt
     5941 agccgcttcc ttatttcaga ctccatattc tggattcaac ccaaaaatta aaactgccga
     6001 caaacttagg ataatcaaag ctggggtcaa gtaagaaatg gcaatggtta ttatttctat
     6061 ttgttttttg aacaaaaaac aaatgaatcc gtacataacc caagtgaggg attcacaatc
     6121 ctggggaaat gggttcaaaa cgtgttctgc cgccacggtt ggcaattaaa tgtctgagat
     6181 tattctgttc tgaccagtgg gtggatcagg tgctgggtga tcttctggaa caatttgagg
     6241 atgattcgaa agggaagggg gcgatgtccg ccagggttct attttggcta aatgtgcttg
     6301 catttgtaag acctcatttc ataaaaaaac aaaaaagaga taaacaaatt ctcatgatta
     6361 ataactacat caaaacttct tacagaaacc tgattaaaag caaagtctat agtattatca
     6421 atttactagg attatccgcg ggaatcagca gtttcttgtt gatttccctt tatctgaata
     6481 atgaactaac ctacgattca ttctttccgg acagcgatcg aatctacagg atttcccttg
     6541 aacgggttta tcctgaccgg accaggctat tcgcgtcttc accggttacg ctggctccag
     6601 tgttaaagga taactacgcg gaagttgaat ccataaccag gatgcacaga gtattttttc
     6661 aaaacgaatt aatggtaacc gttggtgaag acgttttcaa tgaaacaaaa ttttatttcg
     6721 ctgacaaaga tttttttaaa gtgtttcctt acaagtttct ctatggaagc cctgaggagg
     6781 ctttatcaga cccggcaaat gtggtgttaa cccgttccat cgctattaaa tattttggag
     6841 aagaaaatgt tcttggcaga accattaatc aaggaggaga gttaattgtt accggcgtaa
     6901 ttgaagactt gcctaagaat acacatatcc attttgaact gctgggctct attcatgcat
     6961 tgggttacct tcaaaatgca atcgatacga atgcctggat aaatccctgg atatatacgt
     7021 acgtaaaact taagcctggt accggcccgg agaactttca gtctaaattt gatgatttgg
     7081 taagaacttt tggcgcagct tccatttcac aacagatggg ccctgactat gaatctgaag
     7141 gacataagtt taattatttt cttcaaccaa ttcaggacat tcatttacat tcaaacctgg
     7201 acattgaagt ggagccgaac agcaatatcc agtatgtata cttactaagc gcgatcgcaa
     7261 tcgtaatttt acttatttcc agtatcaatt tcattaactt gtcaactgcc aggtcttcgg
     7321 aacgcgctaa agaagtggga atcagaaaag ttcttggctc ttacaaatct aatctggtat
     7381 atcaattcat caccgaatca gtaatgatga gtatggcagg ttcactcctg gcattggcaa
     7441 tagtttttta tgtacttcca tcatttaatt ttattttgaa aaaatctcta accatgggtt
     7501 tctttcttga cccggtgata atcgcgattt ttgtagtttt cataattttt gtgggagttg
     7561 tttcaggatt atatcccgca ttggtaattt cttcgttaca gccctccagc gtattgaagg
     7621 gatcttttaa agccagtaac aaaggtacct tcctgagaaa ctcattaatc attctgcagt
     7681 ttgctatttc aattgtgatg atatcgggtt caataatagt aacacagcaa atgtggtttc
     7741 tgaaggataa ggatttgggt tttgataaag aaaatactct tgttatcagg ggttttcagg
     7801 ccctgggaaa taatgtgaat gcctttaaga atgagatacg gcagttggag gaggttatcg
     7861 cggttggaag ctcgcaagga gttcccggag atttcctggg aagtaacata ttttcgtctg
     7921 atgaagaagg aataacggat ttgcgcgcaa acacagttaa tatcgatgat gatttctttg
     7981 caacaatcgg atttgaattg ttagagggca gggcattcaa tgcggaattc aatgactcgt
     8041 tgaatattat tttgaatgaa tctgctgtga aagatcttgg tttggaaaac ccggtgggga
     8101 aacgaattag aagtgccggt cccagggatc agaaccctga ttttaccata attggtgtag
     8161 tgaaggatta taatttttac tcactccaca gcccaataac tccactggta atttttaaca
     8221 gtagtcccaa caacaatcca cctgtaatgg caattcgaat agctggcgga gattttcaaa
     8281 atactttaaa caaattaaat aaagtatggg ctgaaatggc cactaccggc tcaatcgaat
     8341 ttactttcct tgatcagtac cttgaaaacc agtatgaagc ggatagagct tccggaattg
     8401 tatttgatat atttacactt attgccataa taatgtcatg caccggattg tttggcctgg
     8461 ccacgtatat agtgcagcag cgaaccaaag aaatgagtat tagaaaagtg cacggggcat
     8521 ccctgctaaa tatagtttcg actttttcgc gggagttcgt caaattaatt ttgatagcat
     8581 ttatacttgg agttccgctt gcctattttc tgatggatca gtggctcacc aattttgctt
     8641 accatgtaaa tattagcata gtcacctttc tgctggcggc cctgttcgcg gtagtgttag
     8701 ttttgattac ggtaagctat caatccatca gggtagcatt gctaaatcct attacttcat
     8761 taagaaatga ttaacccgga aaaatgaatc aattttcatt aacatcccgg gaagaaatga
     8821 ttttaatcac ggtgggcgtt ttgcaaccag atgcgtatgc atatgggatt aagaaggaaa
     8881 taaaatctga agccggagct tcaatttccc tggcgaccat tcacactatt ttataccgga
     8941 tggaaaagca gaaattactc caatcttcca tgggagggag ttcagagaaa aggggaggaa
     9001 gaagcaaaag actttataag ctaaccaata aaggctttca caccataaag gaattacaaa
     9061 ccgccagaac ttcgttactg ctgaaattcc aatcaggaaa ttaaatggct gttttttcca
     9121 gccggttcca atctctgata gcatttcatt atcgtcgttt tcaatagttt ttgctgtaag
     9181 taaattgaat ttattctcat cacccacagc gaagaaacca gatccgcctc attgttaaat
     9241 aaagtttacc tgaatgcacg acctgtatta gattaataat tgtagaaaaa ctgccaataa
     9301 agtttccttt tttgtcaaaa tgagtacaaa taaattggtt ctttgccatt ttggcacgga
     9361 ttggaaactt cataattcag cataagatgt gcggaaaaat tcataaaagg ggaattttct
     9421 tggttgcaga gcgaattatg aaattaaagg tttcctgaag gcgtactatc gtatttatgg
     9481 atacaaatgg catgattttg aacctattat tgacaaatca aaacaatatt aattttatga
     9541 tgacttattt aagaaggttt cttttagttg ttggaacagc aattattttg ttctcatgtg
     9601 aggataccgg caatacttcc aggattatgg taacgctgac tgattcaccg ggggattata
     9661 aagaagtaaa tgtccaggtg attggtgtgg aagtacatcg tcaatcgggt gagcagggct
     9721 caggttggat ttcacttgac atggatggcc agacaaatat caatttactt gatcttacca
     9781 atggcgcaac cgctttgctg gctgatgctg aaattccttc agggcagatt tctcaaattc
     9841 ggttgatttt ggggaatgaa aacaccgtgg ttattgaagg tgaaactgaa gatgtaacat
     9901 acgacctcgg cacaccaagc gcaatgcaat caggattgaa actccaggtg catgaaactc
     9961 ttttatcagg tattacctat gagtttaaac tggattttga tgcggctaaa tctgtggtta
    10021 aagcgggaac gtctggtttg tataacttaa aacccgtaat tagggttatc actgaagcaa
    10081 ccagtggagc tatcaaagga agtgttttac cagcggcaga aaacatcgcg gtttacgcca
    10141 tgcagggaga agatacgata gcgacaagct atgcagtaga gaatatctca gagtttctta
    10201 tcggtggctt ggagcccgga acttacaatg tcgtttttga tccgggagag aattccttac
    10261 tccagaccca tacaattgaa ggagtaatgg tttctatagg ggttattata gaattgaacg
    10321 aagtgacttt acaggaataa ttatatatcc gtttagataa aaggtagttt cttctttgaa
    10381 gctgcctttt tctttttcag ggtgatgaag aaaaagatga agtctatcgt ttctgagaat
    10441 aatgccttat ggtatcctga attgccttct cgccagaata cttaggttgg aacccgaagt
    10501 ccttttcggc ttttcctgaa gtgaatgtaa agtttgaaca agtatacagc accgcaaacc
    10561 ggctaagttt gggattgtaa tgtcttaagg agcgcctgat taaagaggtc aatagctttt
    10621 tcaaagtttt cgacatctct ttcgagttgt cccagaaaag caaaaacttc ccctcttgaa
    10681 ttgaagtctt ctcctggcac atgctcatag ccagctcagc ggcttctttg ggcatcctgg
    10741 tggtttcctg attttctaaa agcagccgct tcatttaatt tctcagcaat ttcgctattc
    10801 atgcttaact tttctcaact ttaccatcca atcccaggga taagggtagc ctaaccagga
    10861 gggaggccgc accaattcag ttttccaatc atactcaatt ttatttattg cttcaatgtg
    10921 aagtccgggc ttttcaaata tctgcataag ttctgtgatc agccaatgtt tggttggtga
    10981 accatctaca tcgaaaacac ccatactcac atcgatattc ttacctgtgt aaaatcccaa
    11041 atgttcttcc ggtatttcag aaactgaaaa tccgtcttta ttgtacagtt ccatagttcg
    11101 ccaggttacc atggaagcgg attcccagga tggaatgaca aagattgcgg ttccgtttct
    11161 tttcaatgct ttgcaaacat tgttaaggat ggcgtggttt ttttttacat cgttggaaat
    11221 ggccacgttc acacataaac caaaatcaac ggcaggcaat tgaatgtgtg aattggataa
    11281 atcctttgtc tcacaaataa cgttgccaag atttaagcgg actgcttcct taacacacat
    11341 cggtgaaaaa tccatcgcgt aaactttttt gaaatgtttt gacaaaaggg gtaaagcccg
    11401 cccaactcca catccaaaat ctcccgcaat ttttttgtta cttcctaatc tccggataga
    11461 cttgatcagt aatagattgc ggtcgttctc aaaaacattg aaaaccaatt cttcataatt
    11521 accagccagc ctatcccatt tcgttgccag atcttttgtt gaacgcatga tcatcgataa
    11581 gttaaaaata cttcttgtta aatactaaag tcaataaaat ttaataattg acgggcaaac
    11641 gaaaaaaata acgaaagtaa atccggtaat tacaataaaa tatgagtgag tttaaaattt
    11701 gtgctaagat tttgttacca tcgtaaaact atgctgaatt cctttatgac aattgtaaat
    11761 cagaatagta tcaactttct caattttacc cgtctttatc atgtgtgcag ggacattttg
    11821 atttgtcaaa atctgcatac agagccataa agcggccgaa gttaccgagg aatattctcc
    11881 agtaaaatgt ttaaaacgga cgattggcaa accttgcaat tcttcttcca atgcctgata
    11941 aaaattattg aaacggatat ctccattttc accggtaagc aacattgtac caggaggcag
    12001 ggaatatctc tttataaaat cttttaatct cgcttttaca ataattaaat ctgcggtctc
    12061 aaaaaacaaa ggctcggtta cttttgcaac cgcgttctct ttaatggaat ttactaaaaa
    12121 catagcggca ccttcaccgg ccaggcttcc ttcagtgtta gaatcatata aattgatatt
    12181 gctgcacttt tcctttttat gccatcctcc caacctttca atattatatt gataggtgct
    12241 tatctcttca acccctccta aaagataact atgcgttggg aattctttca gtaacatcac
    12301 ggcttccagc atggcatttt caaaagcgag gccactatga acgtgggtac tgttatgacc
    12361 cgtattctga gacatcattc ccaactgtga ggcaatggca ttgggagtac tttggacaaa
    12421 attcgtgggt gtcagggttc cttcttcata ttcaataatt tgattcagaa atttgatgca
    12481 atcttccatt ccaccatgac cggttccaat tatgattcca tgcaaatctt ttttcagttc
    12541 caaaagaggc aacgcgcatc cgactccata tcgaaccgct tttcccattc ttcgtaaaat
    12601 actggagggt atgcctgggt aagaaggttc ctttacagtt aatttatttt cgaaggaagg
    12661 attaagcgtt tccagatcca tatccggaaa tgtattttgg gaagatatgt aaacagattc
    12721 gtgcagatac ataatcaggt ttttgaaata ataagagtcg tacaattgcc cccaaacccg
    12781 aaagaattag acatcacatg gttaattata agtttctcat tttttgtaac cggaattaat
    12841 ccggtctcct gaatcggtgt tttaaaattg attgcgggaa atacttcctg atgctgcaaa
    12901 tttaaaagac tgtagatagc ttcgatgctt cccgctgcgc caagtgtatg cccaatattg
    12961 gattttgtgc tggaaaatgg tggaggagtt tcaaatactt ttatcatagc cctgctctca
    13021 atcaaatcat tattctcggt tccagtacca tgagtattga cgtagctaat gtcagacttt
    13081 tttaaatgcg ccatttgaag ggcgtgtttc attgaaagga caggaccctt tccctcatcc
    13141 gacaaggaag acggatgaaa cgcgtcattg gtattagcat aaccggtaat ttcgccaagg
    13201 atgggtttgc tgccgactac ttcttcgctc tccaacacca ggaatcctgc accttcccca
    13261 agattaagac cactccggct attatcaaaa ggcatacatt ggcctgctgc aagaatatgc
    13321 agggaattaa atccgttgat agtaaatttt gcaagactgt ccacaccgcc tacaattgca
    13381 cgccttgcaa acccatgttt tatcaaccgg gatccgaaaa tgatggaatt agccgatgag
    13441 ctgcacgcgg tattcatagt attgacataa ccgccaattt taaaatgcgc gatcaggaac
    13501 cgggttgcag ccccggcatc ataggaatca atgaatgggg aagcctcatg atcactattt
    13561 gcatcaaggt ataattcatc agttaggcac atgcctccta ctgttgttgc accaaccagg
    13621 gcagtagttt ccgactcaag ttcgggtttt gtaagtccgc tagagtttat acactcactt
    13681 acggctttta gggcaagcaa tgccgttctt gtaacacctt cattatttat ttcaaattcg
    13741 gaaataatct cttcattcct gattttaatc tccccgaata gcagagattt agtgtaggaa
    13801 gtatttagaa attcagcttt tgatattcca caaattcctg ccctgagtga tttcagattc
    13861 tcttctacgg aattaccaat ggagctgaca aggcctgaac cggttacaaa tacccgtttc
    13921 actctttctt actttgttct gttcacttcg atgtaatcaa ctatggtgtt gatatctatc
    13981 agaatttttc gaccttcctt gggatcattt attttaatgc cgtactttct ctcaagaagc
    14041 acgatcaatt caatcgcatc aatggaatcc aggcctaatc ctgagccaaa aatgggctgg
    14101 tcatctttaa tatcctccgg ttttacatcc atgagattga ggaattcaat gatttgttcc
    14161 tttaattgct gttttagtgc ttctttttcc atcagtacta tatgaggttt ttctttttca
    14221 atccaaaaaa tgccaggact tgcaaaaata aggtaataat aatcagcgga aagagattgg
    14281 gcatcatgtt cgccaatttc cccccttcga ggaataaatc gtgaaatgac tccaggcacc
    14341 agtaaagcgg cgacaagatc ataaaaattc tgaaagattc cggcatggca aagcttggta
    14401 ccagaaggcc gccaatcgcc gcgagtatga atattgagac agccccgaat ccatttgcct
    14461 gttcctgggt tttcgaaaaa actccaacac ataatgcata agacagggca cacaagccac
    14521 aaatcagcga tacaatcagg agcccaacca aatcgttggg tagttccagt ttcggtaatc
    14581 ctactacagg aaataaatac atgccaattg aaaagatcac aattacctga gcaatgctaa
    14641 caaacaggta agtgatttgc tttgacaaaa tacttaccag aatactggtg ggcatggttt
    14701 tcaacctaat aaaactgcca ctcactttct cccttacgat accgcttccc aacgagatga
    14761 caatgaaaaa catggcaaaa atagtccagg cgggaacatt gtgctgggtc gcgtttaaca
    14821 caacatttgc tccatccttg gatgccggta tttcctcaat attggtcttg ttatgcaaca
    14881 actgattttc caactcttcc ggaaggtctt tgttgctgat tttctgataa attgtttcca
    14941 acgtatgccg gttctcaacc aattgcaagg cacttaagac cgcgctttga aaagaatgac
    15001 ggaaagactc ctgtaacacc ggtgtataat ataatttaag tgaattaacg gtatccgata
    15061 cgggactggg tggaacatcg ctgacgccaa aatcaaacat cactctttcg gtaactttga
    15121 gagatttttt ttccaaatcc gcggtgaagg tctccgggat aactaaagcc agaattgcgt
    15181 cattcttatc cataagactt aaaattccac cggattcagt ttcatcatac gtttcaatga
    15241 ttctaaatga acccatagct tttatagact ctctcagttt gtcactaagt tcaccgttgt
    15301 catgatttac tatcagaaat gtaaatctat tctcgttaac taattcaaaa gtgctgtttt
    15361 gaatactggt tataaccaaa accaatacaa tgggcattcc aaacatgagg gtgaggccca
    15421 gtttgtcgcg ggtgaggatt agcaaatctt tccttatgga tgcccaaagt ttaaccatta
    15481 attatctcta taggttttac cggttaattt tagaaatagt ccttcgagac caacttcatt
    15541 atggctcacc aaaagttctt caatagtttg gtgctcaatt atttttccct cgtcgatcaa
    15601 ggcaactttg ttgcacaatt cttccgcttc ccttaataag tgagaagtat aaaccagcgt
    15661 tgttccttca ttattcaaat tcttaaggaa ctctattatc tggtggcggg tttggatatc
    15721 aacgcctacc gtgggctcat caagaaacaa gatttttgga ttatggatta caccaatagc
    15781 aaggttaacc cgtcgtttca tacctcctga aaactgctgc actcttttct tgcgggacga
    15841 tattaatccc aaaatctcca ttaattctgt aattctgctt gtcagttgtt ggcccctgag
    15901 tcccgataaa gtaccgaaga aatccagatt ttctgttgca gttagttctt cataaaagga
    15961 aaattcctgg ggaacataac caaacagggc gttgatttct tttctgttac cgacagggtg
    16021 ttccattaat gaaatacttc ctgtttgcaa tttaaacaaa ccggtcatta aactaagtag
    16081 cgtagtttta ccagctccat ttggaccgaa aaggccaaat ctctccccaa cgatgatgtc
    16141 aaggttaatc ccttccaggc attgcttatt ttggaatgga taacgataat tgacgttctg
    16201 aacagaaact gccttttcag acattaattc aactttactt ttgacaattt cagaaaggca
    16261 tttttttcta cctccgagat atcttccagg taatctccca tttgattgaa atcagcttgt
    16321 gagccgatgc gacctttata aattcctgct gcctgcacag cggctgagcg ccataaatct
    16381 ccagcctttg taaattcttc ggatattttc aaaagctcat cattgggaat gaatttatgt
    16441 gcttcctgca gaaaagcagc ataaataaat ctgaatccac cgccaccggt tccgatttct
    16501 tcctgcatac gaaccaattg agccagataa agtcctgcat cttttacccc cagcttatcc
    16561 cgccattttc tgattctttt tgctgtatat cttatcccac taaccccgac aattggcccc
    16621 ggaatatgaa gcatatcccg cacatttcgt ttgatacctt tgcgaattgc attcgaaatc
    16681 atcgtatcag ttatctgatt tccatttaca acaggataat aaagttgacc ctggggagcc
    16741 aggaatcccc gtgcaaaccg cactctttcc aattcaaaag cggataaccg ggttacagtt
    16801 tccattaccg gatcacttat caggtagtta tcctcttctt ttccgtaaac aattaaatta
    16861 tgagcgttga aatgaaacct gtattccttg gggaaatagc taaggtaata aacaccaacc
    16921 tggcatccca caggttggtt cttccgcaaa atttcattta aaaatttttc ggcactttcc
    16981 tttgaattga atttctttct gaaaaccggg atcccaagtg ccttgcacgt tctcttaaaa
    17041 atatgtcccg gcatggttct aaaggaaaaa gccggaccgt tgcttaccgt gatgaagggg
    17101 atgtaaatga aaaataatcc cgatccgatg ccaaatacca gcggctcgtt caattctctt
    17161 atcccggcga attgcagaag atttgtggta actccgttct cacaatgcgc ggcctgaata
    17221 tgagtaaaat ctaatttcat aatttaatcg ggaggaattg ggtagaatta ttggccaatt
    17281 ttagtctatt ttgaactctt ttcgagtcct ttaaggtatg tttctccgat ttccggattt
    17341 gtttcaaaat ttcgaaggtc atcgatacta atttcaaaca cctcagtata tttctttaat
    17401 ctgtttaccg acattcgtct gaaaggtccg ggcttaaaat gacgttttac tgtccatctc
    17461 caatagccgg tatatgccgc taaagttgga atatccatta ttctcaattc cataaaaaac
    17521 aaaacaggac tagccttccc ttgcattact ttatccctgg cttctgagac tcttgatttt
    17581 atgttttccc aggaaacatc cagggctgat gcctttactt cccacccgcg gcttagtccg
    17641 gttgtatact taccgtcatc gtctgtaaca taggaaactt ctttcgtaaa attatccagg
    17701 acactcggat cctgtggaat atctttcttt ttcattcccc aaaaataatc tataaagata
    17761 caaattatct agcagactgt aaggagtccg tacatgtagg agaaccggct gctttccggt
    17821 actaagagta aaattttatt tcccttttga agtttgccac tgttaaaaag ttcatcaatc
    17881 attaagtaaa tagatgcagc acccacgttg cccaaggtag taagattact aaaccatttt
    17941 tcctggggaa tccccattcc attctcttca agtttctcgg caattttcat tctgaagaac
    18001 tcgcttgaca tatgaggaag aaaataatca acattcttca catcaaaccc attcttatcg
    18061 caggcctctt tcaatttaat tccgcctttt tccacgatat gttcaccaag cagtttaaca
    18121 tcctgcttca tactcagaat tgattgctca atcatttccc tgggtgtata atccatgtag
    18181 cttttcagaa tgcctccttc ttgtttctct ccggccatgt acatgcaggg ttcaatttca
    18241 ttagcgtaag aggcaccggt tagccaatcg atccgaagat tcaacccgct cgtatcgggt
    18301 ttgttctgaa tcttaaaagc tgcggcacca tcggaaagca tccaccggag aaagtctttt
    18361 tcaaaagaga tgtaaggatt gtcttccaga gattccaggc gctgcatttc ttcttcaaat
    18421 acctctgagc gcaatacccg ggaaagtctt tcagaaccgg tggctacggc cgtgttggcg
    18481 taaccttgtt tgacagacat gtatacatac ttcaaagcgt gcatcccact gcaacaaaca
    18541 cctgatggag agactacttc aatagagggc gattcaggaa gccaaccgtg aaccatgcag
    18601 gcatgactgg gcatcatctg gtcaggagtg gaagtgccac aggctaagag ttcaatttca
    18661 ctcagagact ttttttcaaa cagtttcctg atagccaaag aagtcatttc ggcattcgta
    18721 tgcgtgggag ttccgtcctt catcattgca taaaatcttc gctggattcc gttatttctt
    18781 aggactatcc gcttagactt ggattgtctg ccattaatca agcccatgta ttcctctatc
    18841 tcgtcatttg agacgggatt atttggcaga aaattggctg ttttagttat ataaactgat
    18901 tctgtcattc tgatctttta tccaactccc aggtaatatt ccttcttttt ctgaacactc
    18961 ttccccaaaa acggcttaaa taaggtgtta tttattaata atacgaccgg cgcgacaata
    19021 aaaagtgcaa ataaaaggta atatttaaaa ataatcagcc aaaaagttct gtttttgcga
    19081 ttcgaaataa attttgccca aattgaaaac aattttcctg cccggccttc aataaacatc
    19141 aggtttgcct ttacctcaac accgccacaa ctgattatct ccttttgcag accaggcaag
    19201 ttatccgtat taaagtgttc aagggctacc tgtccaaaca ccgaagagtc cctgatatct
    19261 ttttcactta cccccggaac agggaaaatt ccaagataac gatctttctt tcctccgagc
    19321 atccaatgaa gaatgcttac tgcactggcc aggttattat gtttgtcaac taaaacaata
    19381 ttgcctacta actgcccgcc ttcctttgta attaacttct taactttttc ctggctatta
    19441 agccacatat tgcgggctcc aatcaatgta ataaccgcag tattttcaat aaggttgata
    19501 aaatcaggat gttgtaaaat ggaatttacg ggaatgctgg gagaaagaaa ccagggctga
    19561 taggccacga tgatcaattc atatttatca gattttaatt caaatggttt cagttcagac
    19621 ggttttacca gaacgctttc cggcatggca tcaaaaaatt tcttacttgt ccatggaaag
    19681 gggaaatcat ttaccggttc aactttcaga atttcaatct gatgatcact tttttcaaaa
    19741 ggaagcagga aattatccag aatttctttg agttgtccgg actgagaata ataaacagct
    19801 aatattcttt tggacatctt acttcaaatc ctccggcttt agagtttcgg aaaatcttgc
    19861 atactcccgg atgtattttc tggatattgg cgggaggttg ccaaaagaag ttacgattat
    19921 tgacgcatct tcttttttat tattgtcata attaaggaaa cctggagcat tttcctgtgt
    19981 ggctaaccta agaaatctga actcaaggtt ctgagtgtct gacttgatgg agttttccaa
    20041 aagatcccga ccctctttga atgtatccag cttccttttg attgtgggca agcttttgga
    20101 tttcttcatc aggatggcac cgtagaaagc gtctttctca attcctgaat aaggtagtaa
    20161 tgcttcaatt tccctgttga aaagaagggt atcttctgat gaaaaagcct gataataggc
    20221 atcatagttg aaggaacttt gcatgtccct ttgccaggcc atactaattg acgaccatcc
    20281 catgataatg gcaaatgcaa ttcgaaaaag cattgaagat tataaaactt aatgaaaggg
    20341 taaatttaga tcaggttgct ttaattgtct aggaattccg gtcttaatga ggagattaac
    20401 aatcaaaaac ttacctgggt caagtctttc cattctgcga aggtagcagg tatctaaaat
    20461 gatgtgatca ctcatgtcct tctaaatgac aaattaggag cagaaaaggc gcctttttag
    20521 agcgcctttc taaaaaaaat actttgtaat tccttatcct ctcgcttcgt tcaattcaat
    20581 attacaagtg attatcactt cctcacccac tactaatcca ccggcttcca ccgcacgatt
    20641 ccatttcagg ttataatcaa acctgttaat actggctatc actttaaatc cggctttcat
    20701 accacggccg ctgtcgattt taccgttgta cctcacatca aattttacct gcttggttac
    20761 atccttcatg gtgaaatccc ccaccaggaa atatttttca ccttccttgg taatttttcc
    20821 tttgaaagtg atatccggat atttctctac atcaaaaaag ttgtcggaac gaaggtcttt
    20881 atctcttccc tcgttgttag tgtcaacgga tgttgttttt gccgtaaact ctacatccgc
    20941 tccgctaaaa tcttcgcctg cattcctaac agaggcagcg aaatctgtaa attcaccgtc
    21001 aacttcactc accatcatat gtgttacggt gaatttaatg tttgtgtgat ttttgtcaat
    21061 actccaatct gactgagctt tcaaaattcc aaagctcacc agaagtgtca aaagtgtaaa
    21121 ctttattctc attttataat tatttaggtt gaattaaatg ttgcaacatc aagtttacat
    21181 ctttatggat tcaaatcctt acatttttcg cattttcatt aacttgtaag aaaaacaatt
    21241 atcccatgaa aaagttttca ttctccattg caatactaac aatttccttt ttttattttg
    21301 atgttcaggc tcaaagaact caaaagcctg ccttgcatgg ccggcattgg atggccataa
    21361 ccggtaaacc tctggcggca actgcagggg cgaaaatttt tcaccaggga ggcaatgctg
    21421 tcgacgcggc ctgcgcaatg ttagcggcaa cagctaccat gtgggatgtc cttagttggg
    21481 gtggagaaac gcaggccttg atttataatc cgaacacaaa aaaagtgatt gcaataaatg
    21541 cattaggagt cgcgccaacc ggcgcaaccg cggagttcta taaagacaag gggtatgaat
    21601 acccaccaca atatggtcct ctggcggcgg taactcccgg tacaccgggt gggttgctta
    21661 ccatgctggc agaatatggc accatgagtc tgaaagaaat actggagcct gcgatgcaat
    21721 tggctgaagg gtatcctata gaggctcaaa ctgcaaattc gattgaaagg caaaaagaaa
    21781 gaataaaaga atggccatat tcaaaggcgg tttttctcac tcatcagggt gaggaacggg
    21841 aagcaccaca accgggagaa atttttagac aagcagatct gctggctacc ctccagaaaa
    21901 tggtcgatgc cgaacaacag gctctgaaaa aaggtaagaa aagaaaagaa gctatttatg
    21961 ctgcatacga tcgattttat gaaggtgaca ttgccgatga atttgtccga ggggtgcaag
    22021 agcagggagg cttaattaca aaacaagatc tggctaactg gaaagtgtat attgaagaac
    22081 ctgtaatgac aacctacagg gggattgagg tatacaagct caatcattgg gtacaaagtc
    22141 cggtaatgct tcagtctctt aacttgttgg aaaattttga cgtaaaatca atgggatata
    22201 attccgcgaa atacattcat actatttatc aggttatgaa tctggctttt gctgatagag
    22261 atttctacta tggggatccg tatttccctc cagaggaacc aattaaaggt ttgctttcaa
    22321 aggaatatgc aaaagatcgg attaagcaaa tcaattggga aaaaaatgat cccgcggtga
    22381 aacccgggga tccctatcct tttgaaggaa agaaaaaccc gtatttggat ttgcttgaga
    22441 aatggacaaa taagatatcg tattcatacc cccatcgcat catcgttccg gacggcacta
    22501 acaacgcaga tgttaccggc ttctacgcag gtactactac tgtcgaaacc gcagacaaag
    22561 aaggctgggt agtttccatc acaccaagtg gcggctgggt gccggcagta attgccggaa
    22621 aaaccggcgt tggattgagt caacgtgcgc aaagcttcgt gttaaccaaa gaacaaaatc
    22681 cgttcaatgt tattgagccc ggcaaaaggc cgcgggcaac cctgacacca accattgctt
    22741 tgaaggatgg
//
LOCUS       DASWHX010000124         3176 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_1599982, whole genome shotgun sequence.
ACCESSION   DASWHX010000124 DASWHX010000000
VERSION     DASWHX010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 3176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3176
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1599982"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1118)
                     /locus_tag="VGA21_08665"
     CDS             complement(<1..1118)
                     /locus_tag="VGA21_08665"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9650619.1"
                     /translation="MDYLKERLVREISSRKKAEEHAKEAQNILLSGIENFVDVLLFSI
                     DNDYRIRIFNSAFKKATFEAYGINISTGMNLFDFITNESDAIKVKKNVDKALAGDSHM
                     TIEEFGDVEKNYFETRYNPIVNDNKIIGATILSTNVTERERSQIRIKVLNAELEAFAY
                     SAAHDLRAPLRTITGFAGKLINEFGLPIDVLRLLRIIDKNANQMSELIDDLLIFAEID
                     RQAIDKQTVDSQALIDLVIKEEMLHFPNREVKFKFENLQSILCDPKMMKQVFSNLLSN
                     AIKYSGKKISSAITIGSVPLPKEIIFFVKDNGTGFDMKYADKLFDVFQRFHKRSEFEG
                     NGIGLATVKRVIEKHGGRVWAESEESGATFYFSLPANSA"
     gene            complement(1414..1785)
                     /locus_tag="VGA21_08670"
     CDS             complement(1414..1785)
                     /locus_tag="VGA21_08670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650620.1"
                     /translation="MKLLEIIIVTDSPNLRNALFDILKNHLVSGISPVKVFISRHKHL
                     AMAYPELEFIVIDATKAANRKNLISITENLFYLFTHLNAIILLNNAPDRISSMIKDRR
                     VSVISPREIDRLLLSVIKSHR"
     gene            2019..3092
                     /locus_tag="VGA21_08675"
     CDS             2019..3092
                     /locus_tag="VGA21_08675"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9650621.1"
                     /translation="MVQNNDRYTSKIGKHKILILEDSLYDEELVRLELAKISRDFVIQ
                     SAQDRDEFINLIRNFKPEIVLSDFHLKDFNGNEALEITRNNIDAIFIFVSGKIGEEFA
                     IEALKNGANDYVLKGNIEKLQLALTRAIDEKKMKIEREREEKMKELILENISEIIYLI
                     QIDRTTHSIRNLKFISNKIEDVLGFSAQEYEENPALQARLLEVITKSGLLAGKTNGPI
                     LREFKLTKKEGSEIWFEDSLVPVRGQDSAIVTILGSARDITERKGFEEERAAFAQSIF
                     EQNEKLKAFADIVSHKIRGPLARIIGLLNQMQPEDFASNRYLQLVQDEAHLFDKKIFE
                     LAELLEGGGFANTGSNEIPLNND"
BASE COUNT         1011 a          589 c          608 g          968 t
ORIGIN      
        1 gcagaattgg ccggcaaact aaagtaaaaa gtagctccgg attcttccga ctcggcccac
       61 acgcggcctc catgtttctc tataaccctt tttaccgttg caagaccaat accattccct
      121 tcaaattctg accttttgtg aaaacgttgg aatacatcaa agagtttatc agcatacttc
      181 atatcgaatc cggttccatt gtctttaaca aagaagataa tttctttagg tagtggaacc
      241 gatccaattg ttattgccga gctgatcttt ttcccggagt attttattgc attcgagagc
      301 agatttgaaa acacctgctt catcatcttg ggatcacaca atattgattg gagattttcg
      361 aatttaaact tgacctcgcg gttcgggaaa tgtaacattt cttccttaat tacaaggtca
      421 attaaggcct gactgtcaac ggtctgcttg tcaatagcct gccggtcaat ttcagcgaag
      481 atcagcaagt catctataag ttctgacatt tgatttgcgt ttttatcaat aatccgcaat
      541 agtcgcaaaa cgtctattgg caatccgaat tcatttatca gttttccagc aaagccggtt
      601 atagtacgta atggagctct taaatcatgg gcggcggaat aagcgaaagc ctccaactca
      661 gcattaagga ctttaattcg aatttgagat ctttcgcgtt cagtaacatt agtggacaag
      721 attgtagccc caataatttt attatcatta acaatcggat tataccgcgt ttcaaagtaa
      781 ttcttttcta catctccaaa ctcttcaata gtcatatggc tatctccagc cagagcttta
      841 tcaacattct ttttgacttt tattgcatcc gattcattcg tgataaaatc gaacaaattc
      901 atgccagtgg atatatttat tccgtatgct tcaaaagtgg ctttcttaaa ggctgagtta
      961 aagattcgga tacggtagtc attgtcaatg gagaacaaca gaacatcgac gaaattttca
     1021 atgccggaaa gaagtatatt ttgagcctct ttggcatgct cttcggcttt ttttcggctg
     1081 gaaatttctc ttactaatcg ctctttcagg taatccatac ttacctaagt tattacaaaa
     1141 tgagctttgc gaactactta ataatatttc attttatcaa aaaccttaca cggataatta
     1201 aacaagtaga tttgataatt taatatacgg tggcatggtt cataattaga atcaggatca
     1261 taatgtttta gtgaaaaaat tcaacatcag gtgttattcg ttggagggaa aatacaccga
     1321 gctcattcag gttaaatgta cgggagaagt taataaaatt agatgcctga attcctctgg
     1381 ttgttgaatt tgggaccgtg gatgttttcc ttgctagcgg tgactcttta taacagacaa
     1441 tagtaaccgg tcaatttccc ggggtgatat tacgcttact cttctatcct taatcattga
     1501 tgaaatccta tcgggggcat tgttgagcaa aataattgca ttaagatggg taaataaata
     1561 aaataagttt tcggtaattg atatcagatt ctttctatta gccgcttttg ttgcatctat
     1621 aactataaac tccagttcag gataagccat tgccaaatgt ttatgtctgc tgatgaagac
     1681 cttcaccgga gaaatacctg aaacaaggtg gtttttaaga atatcaaata atgcattcct
     1741 caggttgggt gagtctgtca ctatgattat ttccagaagt ttcattttca tttaattttc
     1801 ctttaactat gcaaaaaaag gaataattca aaccacaaaa tacagattac cacctattta
     1861 attcatttaa gtaatttccc tatcgacaaa aaactcaccc tcctctgaaa ttctgtgagg
     1921 atatttgtaa cttaattgat aatcaccgtt gcccaaacgg aattgaaact tcagaagccg
     1981 aattggatgt gcaggtaatt tagtaaacca tacaatgaat ggtacaaaat aacgatagat
     2041 atacctcaaa gattggaaag cataaaattc tgattttaga ggattctctc tatgacgagg
     2101 aactcgtaag gctggaactt gccaaaatat ccagggattt tgtaatccaa tcagcgcagg
     2161 atagggatga atttattaat ctcatccgga atttcaagcc ggaaattgta ttatcagact
     2221 ttcacctgaa agattttaat gggaatgaag cgcttgaaat cacccggaat aatatcgatg
     2281 caattttcat ttttgtgtcc ggtaaaattg gtgaagaatt cgcaatagaa gcccttaaga
     2341 acggagccaa cgactatgtt ctcaagggga atattgaaaa gcttcaactg gctcttacca
     2401 gggcgattga tgagaaaaaa atgaaaattg aaagggagcg tgaagaaaag atgaaggagt
     2461 taatcctgga aaatatcagt gaaatcattt acctgattca aattgatcga actacccatt
     2521 ccatcaggaa cttgaagttt ataagcaata aaattgaaga tgttttgggt ttttccgctc
     2581 aggaatatga ggaaaatcca gctctccagg ctcgcctcct ggaagtgatt acaaagtccg
     2641 gtttgttagc gggaaagacg aatgggccaa tcttaaggga attcaagttg accaaaaaag
     2701 aaggatcgga aatttggttt gaagatagct tagtgcctgt tagaggccag gattccgcaa
     2761 tagtaacgat ccttggctcg gcaagagaca ttacggaaag aaagggtttt gaagaagaac
     2821 gggctgcttt tgcccaaagt atatttgaac agaatgagaa actaaaggca tttgctgaca
     2881 tcgtatcaca taaaattaga ggacctttag cccggataat agggctcctt aatcaaatgc
     2941 aacctgagga ttttgcttca aaccggtatc tacaattagt acaggatgaa gctcacttat
     3001 tcgacaagaa aatctttgaa ttggctgaat tactggaggg tggcggcttc gccaacaccg
     3061 gttctaatga aattcctcta aataatgatt aacacctttc ccaaatagtc aatttctgac
     3121 ttctcgcccg gtaggcaatt cccctaaatt aataaaattg aaaatttgcc aagata
//
LOCUS       DASWHX010000125         8610 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_1215063, whole genome shotgun sequence.
ACCESSION   DASWHX010000125 DASWHX010000000
VERSION     DASWHX010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 8610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8610
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1215063"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2481
                     /locus_tag="VGA21_08680"
     CDS             <1..2481
                     /locus_tag="VGA21_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009186096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX9650622.1"
                     /translation="LAAYGLTPSDVLNAVNSENVELPSGLVEGLFTELTVRTQGRLNH
                     PQEYNDLILKQDENSIVRFRDIGNATLAPENERTILKRDGVPMVMVVLNTQPGSNALE
                     ISEEFHRRMAIIQKDIPEDINATVSLDSSKYIIASINEVKQTIFIAFSLVVLIIFLFL
                     RDWRTTLIPVLTIPISLIGAFFIMYIAGFSINVLTLLGIVLAIGLVVDDTIVVLENIY
                     TKIEGGEEPKQAARKGSTEIYFAVIATTVALASVFLPVIFLQGLTGRLFREFGVVVAG
                     AVIISSFVALSLTPMLSSKILKKRERHNFFYRWTEPFFDGMSNWYNKSLEAFMKFRWV
                     AFLVVLGSAFGIYYGFSSIPTELAPKEDRNRFQIIATGPEGATFEYMDRYVDELNAMV
                     LEEVPEADGIISVTSPGFGSGGVNSAFMFGILKDPDQREKTQSEIVSHITPMVAANTR
                     ARAFVTEPESIGSRGLSSFPVQYVVQAPAVNDLKEVLDIFFDKASQDPTFTFVNVDLK
                     FNKPEITIEIDRERANTLGVSVRDVATNLQLALSGRRFGYFIMDGKQYQVIGQVDRGN
                     RNDPHDLTSLYVRNNGNELIQLDNLVKFKEQSTPPQLYRYNRYAAATFSASLAPGKTM
                     GDGIAAMDRIAGEVLGDKFSTSLSGSSQEFQESSNSLYFAFVFALILIYLVLAAQFES
                     FKDPLIIMFTVPLAIAGALLTLNYFGQTLNIFSQIGIIMLIGLVTKNGILIVEFANQR
                     KARGMNIMEAIVGAAEARFRPILMTSLSTILGILPIALALGAGAQSRTSMGIGVIGGL
                     IFSTILTLYVIPAIYSYFTGKESRVARF"
     gene            2493..3806
                     /locus_tag="VGA21_08685"
     CDS             2493..3806
                     /locus_tag="VGA21_08685"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX9650623.1"
                     /translation="MKTNCFLIAILVTANSFAQDLTLNEAIQIGLENNYSVKIVRNSA
                     SMADNNNTLGNAGFLPILTVGAAQNYTTQNAEQQFITDPNPRVIDAAKSDAFSSNATL
                     SWTIFDGLQMFAAKSRLEELERIGFLNIEKAIQNTIRDISVAYYTIILERDRLNILDS
                     SLLLSRDRLSIAKSKYEVGNASKLEYMTAQVDYNADITAKLRQEEMVLQARIELNRLL
                     NRDLEVQFVVRGQIDVDTTLVMENLVQDAMAKNPDLQILRNNISVSSLSLKEIKAERL
                     PTISLNANYNYNKSNSQAGFLVSNQTNGYSYGASASWNLFNGFTVNKRAQNARIQMST
                     NEMVLQDLQNQLNATLLRTYSHYTFNMKLLSLEQENYLVARENSSIAFERYRLGNSNA
                     LELREAQLQSVQAAGRLIDASYNTKLAELDLLMLSGNIMESENQN"
     gene            complement(3803..5137)
                     /locus_tag="VGA21_08690"
     CDS             complement(3803..5137)
                     /locus_tag="VGA21_08690"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEX9650624.1"
                     /translation="MSRIAQLFRNVNHFLEHDLWKIDVAKLPLYKRLLAQSGKVIFLA
                     YKGFADDKVKMRASALTFFSLLSIVPILAMAFGIAQGFGLDKELERQLINYFQGQEEI
                     LQQSLAYANNLLSTTRGGLIAGVGVVLLIYSVMELLQNIERSFNDIWYINKERPLVRK
                     FTDYLAIMIFAPVLLVISNSLTVFISSEIERLTETVQLIGLFKGLIFPVLRLLPYMVI
                     WILFSLLYVIMPNTKVKLQSAIIAGIMAGSAFLITEWAVIQFQVNVSEYNAIYGSFSI
                     LPLFLVWLQLSWLIVLFGAELSYAIQNIKQYEYEKENVNYSYHNLRMASLLVMNYLSK
                     NFAQGKGPSAFPDLLDDLKIPHRYLQHVLNILVECKLASEVVTKMNFSKFLPAVDVNT
                     LTPVAVIERLNEHGENDPIYKKTNVVDELHSILEGFEQQMRNSDFNKLLKDL"
     gene            5256..5672
                     /locus_tag="VGA21_08695"
     CDS             5256..5672
                     /locus_tag="VGA21_08695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650625.1"
                     /translation="MNRIIAISFLLFMGTGVQAQVNSRDNTAKGSESWRMPLISGGYP
                     VKKFPEAKISGEKPRSRDIGGMKNLSLKEYNKIMDRKIDEYYQRMQAEAKEDRRLERL
                     MKKPQYSDPSYFGHKRKPYKRKLSRRKFCKECGIVH"
     gene            5721..6386
                     /gene="deoC"
                     /locus_tag="VGA21_08700"
     CDS             5721..6386
                     /gene="deoC"
                     /locus_tag="VGA21_08700"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018475397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEX9650626.1"
                     /translation="MEPNRLIEHTNLKPNLTDKQIDQLVREAAENKFLGVCVPPFWVK
                     RAAREIAGKNILLITVIGFPLGYQMTESKITEIEIAIKDGADELDVVMNISAFKTGMP
                     WTKVELAKCSKLIHEAGKIMKVIIETAYLSDQEIIKACGIGSDSGADFLKTSTGFAME
                     GAKPDHIKLMRSVLPANVGIKASGGISTWSKAKEMIDSGADRIGASAGIKIMREFKAN
                     ETS"
     gene            6373..6645
                     /locus_tag="VGA21_08705"
     CDS             6373..6645
                     /locus_tag="VGA21_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020530170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEX9650627.1"
                     /translation="MKHLEIIARGRVQGVFFRASAKDKANQLGISGYVRNQRDGSVLI
                     RAEADAGVLEEFLKWCRHGSPNAIVSELETRELPLANYSDFIIKRF"
     gene            complement(6642..7160)
                     /locus_tag="VGA21_08710"
     CDS             complement(6642..7160)
                     /locus_tag="VGA21_08710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650628.1"
                     /translation="MTVGYSGQKLFPRDRNYILKEAQHQASFRLLRILRDSVIDLYFE
                     IYNPLRLRDDTTIAIETNSEAPVENLLPFYYELAGIYRYKFGKNQLEFLFDGSSHFEK
                     YSREWEETFFDWTKELGRHKPFIRAVLEATVLTHENMRVELIHQRLKWMVAQVFDVKV
                     YKFKGIQELKVA"
     gene            complement(7281..>8610)
                     /locus_tag="VGA21_08715"
     CDS             complement(7281..>8610)
                     /locus_tag="VGA21_08715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650629.1"
                     /translation="PRLGFNYDITGNQNSQVRGGIGLFTSRVPLVWPGGAYNNNGANL
                     GGVSTSNEPFVADVNQQNPTTFDPNNLQYGGDVDLYANDFKIPRVLKMNLGMDQKLPG
                     GIVATFDFIYSKWINDIFYQNINLLPPNQALDLTGTPDTRDRYTSGTAGRIDPGYGRV
                     ILATNVSKGYSYNVSLGLTKTFDFGLGASASYSYGDAYNVFEGTSSQNSSQWRYRQTI
                     SSRNTNQDIGRSIFAPGHRILTMLSFRKEYAKFLGTGINLIWEGRTGNYFSYIIGNSN
                     GMTGVDSGTPELAYIPGNNSEINMSAADYAILNALIENDKYLREHRGEYAERNMSAHR
                     FESILDLRLTQDFFIEMNSGKRNVLQFSLDIFNLGNLLNKEWGKMYQREDDRFNYALY
                     NFAGFAADGTTPTFTAVQYNDNQAYNGNLDDSGLRSSRWQMQFGLRYIFQ"
BASE COUNT         2634 a         1735 c         1778 g         2463 t
ORIGIN      
        1 ctggcagctt atggactaac accttccgat gtgttgaatg cggtaaatag tgaaaatgtc
       61 gaacttcctt ccggtcttgt ggaaggtctg tttactgaat taactgtcag aactcaggga
      121 agactaaatc atccacagga atataatgat ctgatattaa agcaagatga aaattccatt
      181 gtcagattca gggacattgg aaatgcaacc ctggctcctg aaaatgaaag aacaattctg
      241 aaacgtgatg gagtccccat ggtaatggtt gtactgaata cacagccagg atcaaatgca
      301 ttggaaattt cagaagagtt tcatcgcagg atggcaatta ttcaaaaaga tattcccgaa
      361 gatattaacg cgactgtttc acttgactct tcaaaatata tcatcgcatc tatcaatgaa
      421 gtgaagcaaa ccattttcat cgctttctcc ctggtggttt tgattatctt tcttttcctc
      481 agagactggc gaactaccct gattcccgtt ttaacaattc cgataagttt gattggcgcg
      541 tttttcataa tgtatatcgc tggtttttcc atcaacgtac ttactctttt gggaatagtt
      601 ttagccatcg gcctggtagt tgacgatacg attgtggtcc ttgaaaatat ttataccaag
      661 atcgaaggcg gggaagagcc aaaacaggca gccagaaaag gttcaactga aatttatttt
      721 gcagttatcg cgaccaccgt tgccctcgca tccgtatttt tacccgtaat ttttcttcag
      781 ggtttaacag gccggctttt tagggaattt ggcgtggtag ttgccggcgc cgttatcatt
      841 tcctcatttg tagcactttc cttaactcct atgctttcca gcaaaatact aaagaaacgt
      901 gagcgacaca atttcttcta tcgctggaca gaaccctttt ttgatggaat gagtaattgg
      961 tataataaat ctctggaagc ttttatgaaa tttcgctggg tggctttttt ggttgtactt
     1021 ggctcagcct ttggcattta ttacgggttt agttcaatac caactgagtt ggcaccgaag
     1081 gaagatcgaa atagatttca aattattgct accggaccgg aaggtgcaac cttcgaatat
     1141 atggatcggt atgtggatga attaaatgca atggtgctag aggaagttcc tgaagcggac
     1201 ggaataattt cagttacatc tccgggattt ggttccggtg gagttaattc tgctttcatg
     1261 tttggaatat tgaaagatcc ggatcaaaga gagaaaactc aaagtgagat tgtaagtcat
     1321 ataactccaa tggtggctgc aaataccagg gcaagagctt ttgtaactga acccgaatca
     1381 attggaagcc gcggactttc gagttttcct gtgcaatacg ttgttcaggc tcctgccgta
     1441 aatgatttaa aggaagtact ggatattttc tttgacaaag caagccagga tccgacattc
     1501 acttttgtta acgtggacct aaaatttaat aagcctgaaa ttacaataga aattgatcgc
     1561 gaaagagcaa acaccctggg agtttccgtg cgagatgttg ccactaattt acaattggct
     1621 ttaagtggaa gacgttttgg atattttatc atggatggaa agcaatacca ggtaatcgga
     1681 caagttgatc ggggaaaccg taacgatccc catgacttaa cttcattata tgtcagaaac
     1741 aatggcaatg aattaattca actggataac ctggtgaaat tcaaagagca aagtactccg
     1801 ccacaacttt accggtataa ccggtatgct gccgccactt tttcagccag cctggccccc
     1861 ggaaaaacaa tgggtgacgg aattgctgcc atggacagga ttgccggcga agttttagga
     1921 gataaatttt caacttcact ttccggatcg tcccaggaat tccaggaaag ttccaacagc
     1981 ttgtactttg cttttgtatt tgccctgatt ttgatttacc tggttttagc ggcgcagttt
     2041 gaatctttta aagatccatt aatcatcatg ttcacagtac ctcttgccat tgccggtgca
     2101 ctgttaactc tgaattattt tggccagacc cttaatattt ttagtcagat tggaataatc
     2161 atgctgattg gtctggtaac gaagaatgga atcctgattg tagagtttgc aaatcaaaga
     2221 aaggctcgcg gcatgaacat catggaagcc atcgttggag ctgcagaagc cagattcaga
     2281 ccaatcctga tgacaagtct ttcaaccatc cttggaattt tgccaattgc attagccctt
     2341 ggagccggtg ctcaaagcag aacctccatg ggtatcggag ttattggcgg cttgatattt
     2401 tccacaatcc tcacccttta tgtaatcccc gctatctatt cctactttac aggaaaagaa
     2461 agtcgggtcg cgagatttta aataaaagaa taatgaaaac gaattgtttt ttaattgcca
     2521 ttctggttac tgcaaattct tttgctcagg accttacact gaatgaagct atccaaattg
     2581 gactggagaa taattattcg gtcaaaattg ttcgcaattc agcaagcatg gcggataata
     2641 ataatacgct gggtaatgca ggttttcttc ccatattaac agttggcgcc gctcagaatt
     2701 acaccacaca aaatgcagag cagcaattca tcactgatcc gaaccccagg gtaatagatg
     2761 cggcgaaatc agatgccttt tcatctaatg cgacgttgtc ctggaccatt tttgacgggc
     2821 tccagatgtt tgctgcaaag tccaggctgg aagaactgga aaggatagga tttctaaata
     2881 ttgaaaaagc tatacaaaat acaattcggg atatttcagt cgcttattat accatcattt
     2941 tggaacgtga cagattgaat atcctggatt cgagtctcct tttatcccgg gaccggctca
     3001 gtattgctaa gtcgaagtat gaagttggta atgcttcaaa gcttgagtat atgaccgcgc
     3061 aagtagatta caatgcggat attacggcaa agctgaggca ggaggaaatg gtgttgcagg
     3121 cgagaataga attgaaccgc ctgcttaatc gggatctgga ggtgcaattt gtggttaggg
     3181 gtcaaattga tgttgataca accctggtaa tggaaaatct tgttcaggat gcaatggcca
     3241 aaaatccaga ccttcaaatc ttacggaata atatttcagt atcctccctt tctttaaaag
     3301 aaattaaagc tgaaagactc ccaacaattt cattaaatgc caactacaat tataataaat
     3361 ccaattccca ggctggtttt ctggtctcga accaaaccaa tgggtattcc tacggtgctt
     3421 cagcttcctg gaatcttttt aacggtttca cggtaaataa gcgtgctcaa aatgccagaa
     3481 ttcaaatgag taccaatgag atggtattgc aggatctcca aaaccaactt aatgccaccc
     3541 ttcttcgaac ttacagccac tataccttca atatgaaact tctttccctt gaacaggaga
     3601 attacctggt ggcccgggaa aattcctcta tcgctttcga acgatataga ctgggtaatt
     3661 caaatgcact tgaattgagg gaagcccaac tccaatctgt acaggcggca ggtcgtttaa
     3721 ttgatgcttc ctacaatact aaattagccg agctggactt actgatgctc agtggcaata
     3781 taatggaatc agaaaatcag aattaaagat ctttcaataa tttgttgaag tctgaatttc
     3841 gcatttgttg ttcaaaacct tcaagtatgc tgtgaagttc atccacaaca tttgtcttct
     3901 tataaatcgg atcgttttct ccatgctcgt tcagtcgttc aattaccgcc accggagtta
     3961 atgtgtttac atctactgcc ggtagaaatt ttgaaaaatt catcttagtc actacttcgg
     4021 aagcaagctt gcattccaca agaatattta gaacatgctg cagatacctg tgaggaattt
     4081 taagatcatc taacaagtca ggaaaggcgg aaggaccttt tccctgagca aaatttttcg
     4141 acaagtaatt cataaccagt aaacttgcca ttcttagatt atgataacta taattgacat
     4201 tctccttttc gtattcatat tgcttaatgt tctgaatcgc gtatgataat tctgccccaa
     4261 ataaaacaat aagccagctc agctgtagcc acaccaggaa cagcggaagt attgagaaac
     4321 tgccataaat tgcgttatac tcggaaacgt tcacctggaa ttgtataacg gcccactctg
     4381 taattagaaa agcggaacca gccatgatcc cggcgattat cgcggactgt agcttcacct
     4441 ttgtattagg cattatcaca taaagcaatg aaaacaggat ccaaattacc atgtagggaa
     4501 ggaggcgcaa taccggaaag ataagtccct tgaacaaacc gataagttga acggtttccg
     4561 ttagtcgttc gatttcactg gaaataaaaa cagttagact gttggaaata accaataaga
     4621 ccggagcaaa gatcataatg gccaaataat cagtaaactt tctcaccaat ggcctttcct
     4681 tattaatata ccagatgtcg ttgaacgagc gttcaatgtt ttgcaatagc tccattacac
     4741 tatatatgag caacacaaca ccaactccgg caattaagcc accgcgggtt gtactcaaaa
     4801 gattgttggc ataggcaaga ctctgttgca gaatttcttc ctgtccctgg aaataattaa
     4861 tcagttgccg ctcaagttct ttatccaacc caaacccctg ggcaattcca aatgccatcg
     4921 ccaggatggg tacaatggaa agtaaactga aaaaggtaag ggcggaagct cgcattttta
     4981 ccttgtcatc ggcaaaacct ttgtaagcca gaaaaattac tttgccggat tgtgcaagca
     5041 gcctcttgta cagaggtaat tttgcgacat caattttcca gagatcatgt tcaagaaaat
     5101 gatttacatt ccggaataat tgtgcgatgc gcgacatatg ctttatttat tccaaaataa
     5161 cgcaattatt gcgaaatcct aatggagtaa tgggcaacaa atgcaataaa tccagtcaat
     5221 gaaagttatt ttattagaat caattccagc taaaaatgaa tcgcataatt gccatcagtt
     5281 tcttattatt catgggaacc ggagtacaag cccaggtaaa ttcgagagat aatacggcca
     5341 aagggagtga atcctggaga atgccgctta tatctggcgg ttatccagtg aaaaaatttc
     5401 ctgaagcaaa aatctccggt gaaaaacccc gcagtcgtga tatcggagga atgaagaatc
     5461 tttccttaaa ggaatataac aaaatcatgg accgcaaaat tgatgagtat tatcaaagaa
     5521 tgcaggccga ggcaaaagaa gatcgcagac tggagagatt aatgaaaaag cctcaatatt
     5581 cagatccatc ctacttcgga cacaaaagaa aaccgtataa aagaaaatta agtcgaagga
     5641 agttttgtaa ggaatgtggt atcgtacact gagaatagca aatcattatc ttttccgtat
     5701 ttttaggaaa agtgtgatac atggaaccaa atcgcttaat agaacatacc aatttaaagc
     5761 cgaatttaac cgataagcaa attgatcaac tggtaaggga agcagcggaa aataaatttc
     5821 ttggagtttg cgtgccgcca ttctgggtga agcgggcagc tcgcgaaatt gcagggaaaa
     5881 acattttact gataacggta attggttttc ctttaggtta tcaaatgacg gaatcgaaaa
     5941 tcaccgaaat agaaatcgca atcaaagacg gggccgatga gttggacgtg gtaatgaata
     6001 tctcggcatt caaaaccggc atgccctgga ccaaagtgga attagcaaag tgttcaaaac
     6061 taattcatga ggcagggaag ataatgaaag tgattattga aactgcctat ctctccgacc
     6121 aggaaatcat caaggcttgc ggaattggca gtgattccgg cgccgacttt ttgaaaactt
     6181 caacaggttt tgcaatggaa ggagcaaaac cggatcatat caaattaatg agatcagttt
     6241 tacccgcaaa tgttggcata aaagcttcgg gaggtatttc tacttggtct aaagcaaaag
     6301 aaatgattga ttcgggagct gaccggatcg gtgccagtgc gggaattaag atcatgaggg
     6361 agtttaaagc gaatgaaaca tcttgaaatt attgccaggg gcagagttca gggagtattc
     6421 ttcagggcat ctgctaaaga taaagcgaat caattgggga tcagtggtta tgtcaggaat
     6481 caacgtgacg gttcagtcct gatacgtgcc gaagctgatg ctggagtgct tgaagaattt
     6541 ctaaaatggt gcaggcatgg ctctccaaat gccatagttt ctgaacttga aacccgggaa
     6601 ttaccacttg caaactatag tgattttata ataaaaagat tctaagccac cttcaattcc
     6661 tggataccct taaacttata cacctttaca tcaaagacct gggcaaccat ccattttaat
     6721 ctctgatgaa ttagttcaac cctcatattc tcatgggtga gtaccgttgc ctcaagaacg
     6781 gcgcgaatga atggtttatg tctgcccaat tctttagtcc agtcaaaaaa cgtttcctcc
     6841 cactctcttg aatatttttc aaaatgggag ctgccatcaa aaagaaattc cagctgattc
     6901 ttcccgaatt tatacctgta aatacctgcg agttcataat agaaaggaag cagattctca
     6961 acaggcgctt cggaattggt ttcaatggca attgtggtat catcccgcag tcggagagga
     7021 ttataaattt caaagtacaa atcaattact gaatcgcgga gaatcctgag gagccggaaa
     7081 ctggcctgat gttgtgcttc cttcagaata taattcctat ctctgggaaa tagcttttgg
     7141 ccggaatatc caactgtcat tggctgagaa atttgatggt caaagataat aaattcataa
     7201 aaatcgaaag aaaaaactca tttaatgaaa aaagaggccg tttttggcgg cctctttacg
     7261 ttcaaatcta catttatcaa ttactgaaat atatacctta aaccaaattg catttgccac
     7321 ctggagcttc gaagaccgga atcatccagg tttccattgt atgcctgatt atcattgtat
     7381 tgaacagcgg taaaagtagg cgtggttcca tcagccgcaa aacctgcaaa attgtataaa
     7441 gcataattaa acctgtcatc ttctctttga tacatcttgc cccattcttt gttcagaaga
     7501 tttcctaaat tgaaaatatc cagagaaaac tgaagcacat ttctttttcc ggaattcatt
     7561 tcaataaaga aatcctgggt aagtcttaaa tccagaatac tttcaaatcg gtgggcgctc
     7621 atattccttt ccgcatattc tcccctgtgt tcacgcaaat atttatcatt ttctataagc
     7681 gcattcagga ttgcataatc cgcagcagac atattaatct cagaattgtt tcctggaata
     7741 taagcaagct caggtgtacc ggagtcaaca ccggtcattc cattggagtt tccaattatg
     7801 taactgaaat aattaccggt tcttccttcc caaataagat ttatgccggt ccccagaaat
     7861 ttggcgtatt cttttctgaa acttaacatg gtaagtattc gatgacccgg agcgaatatt
     7921 gaccttccaa tatcctgatt tgtatttcgg gaagagatgg tttgcctgta tctccattgt
     7981 gaactgttct gagacgacgt tccttcgaat acattgtaag cgtcaccgta agaatagctc
     8041 gcggaagctc caagtccaaa gtcaaaagtc ttggttaagc cgaggcttac attatacgaa
     8101 taacccttac ttacattggt ggccagtata actctaccgt aaccgggatc tatgcgccct
     8161 gccgttcctg aagtatatcg gtcgcgcgta tcaggagttc cggttaagtc cagggcctgg
     8221 ttaggcggaa gcaggttaat gttctggtag aaaatgtcat taatccattt gctgtaaata
     8281 aaatcaaatg tagccacaat cccgccgggt aatttctgat ccatccctaa gttcattttt
     8341 aagaccctgg gaattttgaa atcattggca taaaggtcaa cgtctccgcc atattgcaag
     8401 ttatttgggt caaacgttgt aggattttgt tgattaacat ccgcgacaaa tggttcatta
     8461 cttgtactca caccaccaag atttgcgcca ttattattgt atgctcctcc gggccataca
     8521 agcggaaccc tgctggtaaa taaaccgata ccgccgcgaa cttgagaatt ctgatttccg
     8581 gtaatatcat aattaaatcc tagtctgggc
//
LOCUS       DASWHX010000126         4965 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_3422958, whole genome shotgun sequence.
ACCESSION   DASWHX010000126 DASWHX010000000
VERSION     DASWHX010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 4965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4965)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4965
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3422958"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(408..578)
                     /locus_tag="VGA21_08720"
     CDS             complement(408..578)
                     /locus_tag="VGA21_08720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650630.1"
                     /translation="MKYINLPASELQELALQILEECDKDQDYYPFILFMEIYRSEVME
                     VLDKMERNEFPI"
     gene            1007..1297
                     /locus_tag="VGA21_08725"
     CDS             1007..1297
                     /locus_tag="VGA21_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008627904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEX9650631.1"
                     /translation="MRQSIDIETKLQELKPVLLDKFHVSKIGYFGSCAAGGQTDKSDL
                     DLLVEFSQPIGWEFFTLEKFLEQEFGLRIDLVTKDALKERIKRPILNQVKYV"
     gene            1294..1653
                     /locus_tag="VGA21_08730"
     CDS             1294..1653
                     /locus_tag="VGA21_08730"
                     /inference="COORDINATES: protein motif:HMM:NF014043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HEX9650632.1"
                     /translation="MKPSERDNILYLEDIVLAMDRIHEYISGLDFIQFKRDYKTVDAV
                     IRNFEVIGEASKNLSKDIKDNFSNVPWEEMYRLRNRISHEYFGVDYEIIWDIISRHLP
                     NNYRDIKVILDNERQKE"
     gene            complement(1892..4042)
                     /locus_tag="VGA21_08735"
     CDS             complement(1892..4042)
                     /locus_tag="VGA21_08735"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX9650633.1"
                     /translation="MKTKAHTIDRREFIKLSAVSGAFLAVGCLPSPGKETKIVNLADA
                     SGLGKEINQYIFINDAGKITLFNHRPEMGQGTFQSIPMIIAEELEVDISAVEIKQSPA
                     NRELYGDQMVVGSRSMRGNFDLMRKMGAAAKEMLIKAAANKWKVSESDCYAENAFVIN
                     KSSGEKLSYAELVEDASKLSAPGNPKLKSTSEFKVLGQNYPRRDIPSKTNGTAKFGMD
                     MTTPGMLYATIERSPLFLAKIVRFNADNALKVPGVKHVFKTSREVWGNEREGVAVLAE
                     NYWAAVQGRRVLEIEWDNKEFEKNTSESLLKESKEASEQPGNVFESSGDFEKAFNDAA
                     TKVESAYETPYQSHAPMEPMNAIVSVTDNKVEFWGSTQNPNGIKSFLSRKTGVPESKV
                     IINYTFMGGGFGRRSMTDVAEEAADLSMKSKAPVKVIWTREDDITQGPFRAASYNVAK
                     GGLDKDGNLVALEHKVICQDIRNQTGGNNTASGSIAGGIVTDYAIPNFRINGVLRKFY
                     IPISYWRAVYHSTNTFAHECFMDELARAGKKDPLAFRLDLLKNHRRFSKVLQEVAGKS
                     GWNTPKDTNTGRGLAIVERSGAFVAMVAEVHQTNNKIAIRKFTTAIDCGIPVHPDTII
                     GQTEGCIIMGLTATFNGLTVVNGAIAEGNFDTYKMLQLRDSPEMEVSVIDSTDAPEGA
                     GEAALPTVAPALVNAIFDLTGKRIRKLPFKLEEV"
     gene            complement(4055..4522)
                     /locus_tag="VGA21_08740"
     CDS             complement(4055..4522)
                     /locus_tag="VGA21_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019943688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX9650634.1"
                     /translation="MSSIKLKINGKEYPMELDPQTPLLWAIRDYAGLTGTKFGCGIAQ
                     CGSCIVHLNGNAVRSCIVPVSAASNTEILTIEGLSENNDHPVQKAWIEEQVPQCGYCQ
                     SGQIMAAAALLKRNPDPSDEEMYTGMQGVICRCGTYPRIKNAIVKAAQLIKES"
     gene            complement(4773..>4965)
                     /locus_tag="VGA21_08745"
     CDS             complement(4773..>4965)
                     /locus_tag="VGA21_08745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650635.1"
                     /translation="NNELIWDRSFGRPDNAVVLPDGWYLTTNSIPATIDQTEDGKTRL
                     HYINDRPEEIDVFIKARRK"
BASE COUNT         1405 a         1100 c          961 g         1499 t
ORIGIN      
        1 aaagtgaagc caggcttgag gttggaaact ctcttttggt tttcctaaaa aagaatggac
       61 gcagataaaa ctttaataaa attagttcat cagggcttga ctttcaacat agaagagtcc
      121 cttcggagac tctgaggggg agtaaaagaa tcatgaagcg catcaccgga caacaacctt
      181 tcgtgtggag tacattgctt tactgacaga atggtatttc atatgaagag aaatatttgt
      241 tatagttaaa cccctgcggg gttgttatca gggattttag tatgcacggc atttcatgcc
      301 gtgttattaa aaattcaatc ccttcgggat taatccggat tgctatgaga aaagaaaagc
      361 atcagaacac taatgtcttc gctcagtctg cactgtgacc accggactca tattggaaac
      421 tcattccttt ccatcttgtc cagcacctcc atcacttccg aacgatagat ttccatgaag
      481 agtataaaag gatagtaatc ctgatcctta tcacactctt ccaggatttg cagtgctaac
      541 tcttgtaatt cgctcgcggg tagattaatg tatttcatat acttaaataa aatagcgtgg
      601 tacgtgaact tatcccttag ggaggtgcac cacacaccta caatgcagat aagccacgcc
      661 cacgcgtacc gcgagcgatg tggccttctg ttcttgcatt gtattagtaa gtggtgcttc
      721 cctgagaaga accgaagccg acgctttgtc gttcagtatt tttctttgtt ctaaagataa
      781 ggaaattatt aattctaaag atgggaaaat tattgaccac ccctaacctt aaaataagga
      841 aaggcaaatt aagtaaccat cccaccagaa tatcattctg atttctgccg aaactcaaac
      901 aaatgtaaac ttcatagcaa aaccgacttg tcccggggat tacattatag ttactgccaa
      961 tgccctcaga cgacaaaaaa tcccaagatt ttggtaaatt tgactcatga gacaatctat
     1021 tgacatagag accaagttac aggagttaaa acctgtttta ttggacaaat ttcatgtaag
     1081 taaaatcggc tattttggtt catgtgctgc tggtgggcag acagacaaaa gtgacctcga
     1141 cttattagtt gaattttccc aaccaattgg atgggagttt ttcacccttg agaagttcct
     1201 tgaacaagaa ttcggactga gaattgactt ggtgacgaaa gatgctttaa aagaaagaat
     1261 caaaagacca attttaaatc aagttaaata cgtttgaaac ctagcgagcg ggacaatatc
     1321 ctatatcttg aagacatcgt attagcgatg gaccgaattc atgaatatat ttcaggactg
     1381 gatttcattc aattcaaaag agactacaag actgttgacg ctgtaatcag aaactttgaa
     1441 gttattggag aagctagtaa aaatttatcc aaggatatca aggacaattt ctcaaatgtt
     1501 ccttgggaag aaatgtacag acttagaaat agaatatccc atgagtattt cggggttgac
     1561 tatgaaataa tttgggacat catttcaaga catttaccga acaattacag ggacattaaa
     1621 gtaattcttg acaacgaacg acaaaaggaa tagggcacag gcggcaacat attcaatttc
     1681 gttccttaaa tttagtctta caacggtgtt gcggccaggt cgggtttaac cttccactgc
     1741 gctccatttt taaaccctat acccgcaact acttaaacgc aaaacgatat atattaattt
     1801 ccatgcattt ctgagggcgt gtcatccttc ataatgacga cgatgccaat ctgatctaaa
     1861 taaccggatg aatactgaaa caagttcggc gttaaacctc ttccaactta aatggcaact
     1921 tccggattcg ctttccggtc aagtcaaaaa ttgcattgac taacgcgggt gccacggtag
     1981 gcaaagctgc ttcccctgct ccttccgggg catcggtact gtcaataacc gatacttcca
     2041 tctcaggtga atcgcgcaat tgaagcatct tgtaagtatc gaaattacct tcagcgatag
     2101 caccattaac cacggttaaa ccattaaagg tcgctgttag tcccatgatt atgcagcctt
     2161 ccgtctggcc aataattgta tccggatgca cgggaattcc acaatcaata gcggtagtaa
     2221 atttacgaat ggcaatttta ttattagttt gatgcacttc cgcgaccatt gcgacgaatg
     2281 cccctgaacg ttctacaatt gctagtcccc ttcctgtatt cgtatctttt ggtgtgttcc
     2341 agccggactt cccggctact tcttgcaaaa ctttgctaaa cctccggtgg tttttcaata
     2401 aatctaatcg aaatgccaaa ggatctttct ttcctgcccg ggccagctca tccataaagc
     2461 attcatgagc gaaagtattt gttgaatgat acactgcccg ccaatatgaa atcgggatgt
     2521 agaactttct gagtacgcca ttaatccgga agtttggtat tgcataatcg gtcacaatac
     2581 ctcccgctat agatccgctt gctgtgttgt ttccaccggt ttgattgcgt atatcctggc
     2641 aaattacttt gtgctcaagt gcaaccagat taccgtcttt atcaagtccg cctttggcca
     2701 cattgtacga ggccgcgcgg aatggtccct gcgtaatatc atcttcacgg gtccaaataa
     2761 cttttaccgg agctttggat ttcatgctca ggtcggcggc ttcctccgcc acatccgtca
     2821 ttgaccttct gccgaaaccg ccacccataa atgtataatt tatgatgacc ttgctttccg
     2881 gtactccggt tttgcgcgac aagaatgact taatgccatt gggattttga gttgaacccc
     2941 aaaattctac cttgttatca gtaacagaaa caatagcatt cataggttcc attggagcat
     3001 gcgactggta gggagtttca taagcggatt ccacctttgt tgctgcatcg ttaaaagctt
     3061 tttcaaaatc accggaactt tcaaaaacat tgcccggttg ctctgatgct tcctttgatt
     3121 cttttagcaa gctttcagaa gtgttctttt cgaactcttt attgtcccat tcaatttcca
     3181 atactcttcg gccctggacg gccgcccaat aattttccgc taaaacggct actccttcgc
     3241 gctcattacc ccacacttct ctgctggttt tgaaaacgtg cttcacaccc ggaactttca
     3301 gcgcattgtc agcattaaac ctgacaatct ttgccaggaa taacggagat cgctcaatgg
     3361 tagcatacag catccccggt gttgtcatat ccattccgaa tttggcggtt ccatttgttt
     3421 tggagggtat atcccgcctt gggtaatttt gcccaagaac cttaaactcc gaagttgatt
     3481 ttaatttggg attgccgggc gcagataatt ttgatgcatc ttcaactaat tccgcataag
     3541 acaatttttc accggaagat ttatttatga caaatgcatt ttctgcatag caatcacttt
     3601 cggaaacttt ccatttatta gctgccgctt taatcagcat ttcttttgcc gcagcaccca
     3661 tctttcgcat caaatcaaaa tttccgcgca tgctccggct gcccactacc atctggtcac
     3721 catataattc cctgttggcg ggggattgtt taatttccac tgcgcttata tcaacttcca
     3781 gttcttcggc aataatcatc ggaattgatt ggaatgtgcc ctgacccatt tccggccggt
     3841 gattgaaaag cgtaatttta ccggcatcat taatgaaaat atattgattg atttccttgc
     3901 ctaatccact tgcatccgca aggtttacaa ttttggtttc cttacccgga gatggcaggc
     3961 aaccaacagc cagaaaagct ccggatactg ctgagagttt tataaattcc cttctgtcga
     4021 tggtatgtgc tttggttttc atggaagact tcggttaaga ttctttgata agttgagcag
     4081 ctttgacaat agcattttta attctcgggt acgttccgca acggcaaata actccctgca
     4141 tccccgtata catttcctca tcagatggat cggggttcct tttcaataag gcagccgcag
     4201 ccataatttg ccctgactga caatatccgc attgcggaac ctgttcttct atccatgctt
     4261 tttgtaccgg gtgatcattg ttttctgaaa gtccttcaat ggtgaggatt tctgtgttag
     4321 aagctgcgga aacaggaaca atacaactcc ttactgcatt tccattcaaa tggacgatgc
     4381 aacttccaca ttgagcaata ccgcacccga atttagtgcc ggtaagcccg gcgtaatctc
     4441 taatcgccca taataggggt gtttgaggat cgagttccat aggatactct tttccattga
     4501 ttttcagctt tattgaagac ataagcctga ttttttatga ctattaaatt agctaaaaag
     4561 tgaggggtcg aaaagaaagt ggcaggctaa tgatgctttc gatattaata ttcccttaga
     4621 ttcaccatta atcattagta aagtacaagg atgcaggttg gcaaaaaaat aatgattata
     4681 ccgcctccgg aggttataca agaaaatcta tattccagat tttactttaa tgtaaacagc
     4741 gtaaatacct tattatcact ttcttctcgt tatcattttc ttctcgcctt aataaataca
     4801 tctatttctt cgggtctgtc attaatataa tgcaacctgg ttttaccatc ttcggtttga
     4861 tctatagtcg ccggaattga attggtagtt aaataccagc catcaggtaa tacaacagca
     4921 ttatcaggac ggccaaagct gcgatcccaa ataagttcat tatta
//
LOCUS       DASWHX010000127         6256 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_1215885, whole genome shotgun sequence.
ACCESSION   DASWHX010000127 DASWHX010000000
VERSION     DASWHX010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 6256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6256
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1215885"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            368..664
                     /locus_tag="VGA21_08750"
     CDS             368..664
                     /locus_tag="VGA21_08750"
                     /inference="COORDINATES: protein motif:HMM:NF017935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF983 domain-containing protein"
                     /protein_id="HEX9650636.1"
                     /translation="MFPYKTYNLRKFYKMEPRCPVCNRSFIPESGFYTGAMYFSYAIN
                     VILLLFSGLLATKFLDSSRLLWIILISMVPSLLLAPLTYRISRSLMLHLLGYTP"
     gene            730..1389
                     /locus_tag="VGA21_08755"
     CDS             730..1389
                     /locus_tag="VGA21_08755"
                     /inference="COORDINATES: protein motif:HMM:NF014724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ElyC/SanA/YdcF family protein"
                     /protein_id="HEX9650637.1"
                     /translation="MRTLPFSLILCIATLTCSCAFSPRKTLQYYLQAQKTRYDIIVVP
                     GVPLENGRWSWTMKGRIYWAKFLFDNGITENIMFSGSSVYEPYVEGEVMALYAEAIGI
                     PKEYIYAEKKAEHSTENIYYGYHKAKMLGFEKIALASDPFQTKSLKRFTRVKVHPSVG
                     LIPMVIDTLEAMQEVMFDPVVDLSKARVENFTSILERESFFKRLKGTMGKNLDTTAYR
                     K"
     gene            1619..4432
                     /locus_tag="VGA21_08760"
     CDS             1619..4432
                     /locus_tag="VGA21_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013452711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEX9650638.1"
                     /translation="MKKGTNLILFLLFVTGFVFSQETKITGNLDSKIPVDPSITVGKL
                     DNGLTYYIKSNPKPENRVEIRLVVNAGSLMEDDDQKGIAHFVEHMAFNGTKNFEKNEI
                     VNYLQSIGVQFGADLNAYTSFDETVYILPIPTDDPAIVEKGFQVIADWASNVLFTDEE
                     IDKERGVVVEEWRLGRGANQRMRDQYFPILFKNSRYAERLPIGEKEVIENVSYERVRQ
                     FYKDWYRPDLMAVIVVGDVDKNEIKVKIEEYLGGIEKPKDPRPRQSHEVPDHKETFIA
                     ITSDKEATFTQIQLLYKTDNVESVTLKDYRRDLIYQLYNGMLNERLQELTQKPEPPFL
                     FGSTSFGSMVRTKSSYSSFAVVGENGIAAGLKTLIEENERVKKYGFTPAEFERYKLEM
                     LDRYERAFKESDKTESGSYASEFIRAFLTNEPIPGITNEYEYVKNFLPGIKISEINGL
                     AAQWIKDTNRVVIITGPQKEGVKMPAEGEIRTMLDEASKMEITPYEEAELATALMEQM
                     PSPGKVVDSKFYDNVGITELSLSNGAKVVLKPTDFKNDEILMQAYSFGGNSLYIDADY
                     QSASNASALISESGVNGFSPTDLQKLFAGKSVSASPFIGTLSEGMRGNAAPKDFETLL
                     QLMHLYFTEPNKDQAAFTSMITKNKMLYQNLMSNPNYYFSNEVNKILTQNHPRGGGFP
                     TMEDLEKIDFEKAFSIYKDRFADAGDFTFFFVGNFNIAEITPLLEQYLGSLPSNNRSE
                     TWKDLGVRPPNGVVNEVVRKGEDPKSQVIITFTGEKEYDKKSNYDLSSLGEMLSIKLI
                     EILREEKGGVYGVGAFGGSSKYPVEDYTFRISFPCAPENVDDLVKAVFDEIELIKQEG
                     VSDEDLTKIKETQRRDREEGLKQNRYWLAQLNAYYQNNTGLDTFFDREELTNTITSAD
                     LKNAAKKYLRMDNYIQVVLKPEN"
     gene            complement(4443..5630)
                     /locus_tag="VGA21_08765"
     CDS             complement(4443..5630)
                     /locus_tag="VGA21_08765"
                     /inference="COORDINATES: protein motif:HMM:NF013890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650639.1"
                     /translation="MKFIISLILSITTVISFGQIQPGFEKQEARDMIALCNSFTFMEL
                     YNSDSKIIPAHYEKVYTSGIFGMDNKFQIYRKGKVAVINLRGSTDDMSSWLENMYASM
                     IPANGTIKTAGETFAYSFAKDTSAAVHAGYALGIVYLSNDIKYHIDNFNRDGIYDFII
                     TGHSQGGSLAIMLRAYLENLPEGVISPKNKFKTYAFAHPMVGDKEFSAEYNKRFCINE
                     TGYSVVNPKDLVPKFPLAYNDENYVADNLMKLFDDKESFDPRRLALEGVLNRFESFIG
                     KSMQYMGNSISEQLKRELGHVEMPGFVKEINYSKIENRVEIPPVEYPLILKDPTILKN
                     DSLMAVLPRDDKGNFTDRNLYEKEGIFYQHKPYNYYVSLLKTFFPKDYDALELKFLPE
                     NLR"
     gene            5814..>6256
                     /locus_tag="VGA21_08770"
     CDS             5814..>6256
                     /locus_tag="VGA21_08770"
                     /inference="COORDINATES: protein motif:HMM:NF039391.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6265 family protein"
                     /protein_id="HEX9650640.1"
                     /translation="MKIAKRVSSGMILIFFLALTNHLWAQENFPNTLFLGEEETSPSA
                     TIDNMSWIAGYWQGEAFGGIVEEFWTAPSAGNMVCVFKLIVEGKTLLYELCTISEENN
                     TLMMRLKHFNSKLIGWEDKDKSIDFPLVKMEKNKIYFDGLTFERI"
BASE COUNT         1993 a         1158 c         1283 g         1822 t
ORIGIN      
        1 tgcggttgct atataaggta attaccccct gtagtccccc ttaaaacaag ggggacaatt
       61 tttctgggca gggggtactt tcccctcctt aatttaagga ggggctaggg gtggttctta
      121 aaatggtatt caaaccccct gtagtccccc ataaaaacaa gggggacaat tttccatggc
      181 ggagacactc tcggtttctc agtatgataa ttggagttga aattatctta actttgccag
      241 tgattcaact cataaggaat tgtaatttga aatcagttta cgtcaatcaa tacactataa
      301 ataatacata tcaatgaact ggaaccggat gctggaatgc aaatgtcctc agtgcggaaa
      361 cggaaggatg tttccctata aaacctataa tttgagaaaa ttttataaaa tggaaccaag
      421 atgtcctgtg tgtaaccggt cttttattcc tgaatccgga ttttacacag gggccatgta
      481 tttcagttat gccataaatg taattctact actgttctcc ggtttattag caacaaaatt
      541 ccttgacagc agcaggcttt tatggataat cctgattagc atggtaccat cactactcct
      601 tgctccgctc acttatagaa tttcaagatc cctgatgctt catcttctcg gatatactcc
      661 ctaaacgccc tctcaatgaa actattcagg ttcttaacct gtttatctat aaggttgaaa
      721 attaaaaaaa tgcgaacctt gccattttct ttaattcttt gtattgccac tttaacatgc
      781 tcctgcgctt tctcgccgcg aaaaaccctg caatattacc tacaggcaca aaaaaccagg
      841 tatgatatca ttgtagtgcc cggagttcct ctcgaaaatg gcaggtggag ctggacaatg
      901 aaaggcagga tttactgggc aaaatttctt tttgacaatg gcatcacaga gaacatcatg
      961 ttttcaggtt cttcagttta tgaaccttac gtggaggggg aggtgatggc cttgtatgct
     1021 gaagccatag gcattcctaa agaatatatt tatgcagaaa aaaaagctga gcattctacc
     1081 gagaatattt attatggtta tcataaagcc aaaatgcttg ggtttgaaaa aattgccctg
     1141 gcgtctgatc cctttcaaac caaatcactc aaacgtttta ccagggttaa ggttcatcct
     1201 tcggtaggac ttatacctat ggtaattgat actttagaag cgatgcagga ggttatgttt
     1261 gatcctgttg ttgatttaag caaagctcgt gtagaaaatt ttacttctat actggaacgc
     1321 gaaagtttct ttaagagatt aaaaggtaca atgggcaaaa atcttgatac tactgcctac
     1381 cggaaataat taaaagcaaa ttggcgtgtc aatgtagtct ccaccatttc ttcaaccatt
     1441 ccatgaaatt gcagatagct tctgctttta atcaatcggg caatttatcc ttcagaattc
     1501 ctgataaaaa tgacaatcgt catactttat gtaacctgat attaccgtta tttgatacat
     1561 ttattatagc aaccttccct taatctcatt aattttatgg tctactaatt tatcaatcat
     1621 gaaaaaagga accaatctta ttctattctt attatttgtt accggatttg ttttcagcca
     1681 ggaaacaaag attaccggga atctggacag taaaattcct gtagatccca gtataacagt
     1741 tggaaaacta gataatggac ttacttatta tataaagagt aatccgaagc ctgaaaaccg
     1801 ggttgaaatc cggctggtgg ttaatgccgg atccttaatg gaggatgatg atcagaaggg
     1861 gatcgcgcat tttgtggaac acatggcttt caacggtacc aaaaatttcg aaaaaaatga
     1921 aattgttaac tatttgcaat cgattggtgt tcaattcgga gcagacctga acgcttatac
     1981 aagttttgat gaaactgtat atattctccc tattccaact gatgacccag ccattgttga
     2041 aaagggattt caggtaattg cagactgggc ttcaaacgtt ttatttactg atgaagaaat
     2101 agataaagaa cgcggcgttg tagtagaaga atggagattg ggccgcggag cgaatcaaag
     2161 aatgcgtgac caatattttc caattctatt caagaattcg cgatatgcag aaaggcttcc
     2221 tattggagaa aaagaagtaa ttgagaatgt aagctatgaa agagtgcggc aattttataa
     2281 ggattggtat cgtcccgacc ttatggcagt cattgttgtc ggtgatgtgg ataaaaatga
     2341 aataaaagtt aagattgaag aatacctggg cggaatcgaa aaacctaaag acccaaggcc
     2401 cagacagtcg cacgaggttc cggatcacaa agaaacgttt atcgctatta ccagtgataa
     2461 agaagcaacg tttacacaaa ttcagctgtt atataagacc gataatgtgg aatcagtcac
     2521 actcaaggat taccggaggg atttaattta tcaactttat aacggcatgc ttaatgaacg
     2581 gttgcaggaa cttacccaaa aaccagagcc tcctttcctt tttggcagca caagttttgg
     2641 tagcatggtc agaacaaaaa gtagctactc ctcatttgcc gtagtgggag aaaatggaat
     2701 agcggccggc ctgaaaacat tgattgaaga aaatgaacga gtaaaaaaat acggttttac
     2761 tcctgcagag tttgaaagat acaaactcga aatgcttgat aggtacgaac gcgcatttaa
     2821 agagagcgat aaaacagagt cgggatccta tgccagtgag tttatccgcg ctttcctcac
     2881 caatgaacct attccgggaa tcactaacga atatgaatat gtgaaaaatt ttttgccagg
     2941 gatcaaaatt tctgaaataa atggtctggc ggcacaatgg ataaaggata ccaacagggt
     3001 agtaattata actggtcccc aaaaagaagg ggtaaaaatg cccgccgaag gggaaatcag
     3061 aaccatgctg gatgaagctt cgaagatgga aatcacacct tatgaagaag cggaactggc
     3121 aaccgcgttg atggagcaaa tgccttctcc aggcaaagtg gtggattcta aattctatga
     3181 taatgtgggc attactgaat tatcattatc aaatggtgca aaagtagtac ttaagcctac
     3241 tgattttaag aatgatgaaa tcctgatgca ggcgtacagc ttcgggggga actcattata
     3301 tattgatgcg gattaccaat ctgcatcaaa tgcttccgct ttaatttctg agagtggcgt
     3361 aaatgggttt tctcctactg atttgcaaaa attgtttgcc gggaaatcgg tttcagcatc
     3421 gccctttatt ggaactcttt ctgaaggaat gcggggaaat gccgcaccca aagattttga
     3481 aacgcttctg caattaatgc atctttactt cacagaaccc aataaagatc aggcggcatt
     3541 caccagtatg attacaaaaa ataagatgtt gtaccagaat cttatgtcaa atccaaacta
     3601 ctatttctcc aatgaggtga ataaaatttt gacgcaaaac catccccggg gcgggggatt
     3661 tccgactatg gaagatttgg aaaaaataga tttcgaaaaa gcgttttcaa tatacaaaga
     3721 tcgctttgct gatgccggtg acttcacttt cttcttcgtg ggtaatttta acatcgcgga
     3781 aattactccc ttacttgagc agtatctcgg aagtttaccc tcaaataacc gcagcgaaac
     3841 ctggaaagac ctgggcgtaa ggccaccgaa cggagtggtt aatgaagtag ttagaaaggg
     3901 cgaagatccc aagagccagg taatcataac atttacaggt gaaaaagaat atgataagaa
     3961 aagtaattat gatttgtcat ccctgggtga aatgctgtcc ataaaattga ttgaaatttt
     4021 gcgtgaagaa aagggcggag tttatggagt aggagcgttt ggcggctctt caaaatatcc
     4081 agttgaagat tatactttca ggattagttt cccttgtgcc cctgaaaatg tggatgatct
     4141 cgttaaggcg gtttttgatg aaatagaatt gataaaacaa gaaggcgtct ctgatgaaga
     4201 tttgactaaa atcaaggaaa cgcagcgcag agatcgcgaa gaaggtttga aacaaaacag
     4261 gtattggctg gcacagctga atgcatatta tcaaaataat accggtttgg atacattctt
     4321 tgaccgggag gaattaacca atacgattac ctcagctgat cttaaaaatg cggcgaagaa
     4381 atatctccgg atggacaact atattcaagt ggtgctgaag ccggagaatt aatttaataa
     4441 gatcacctca gattttccgg caggaatttt agctcaaggg catcataatc tttagggaag
     4501 aaggttttta gtaaggaaac gtaatagtta tacggcttat gctggtaaaa aattccctcc
     4561 ttttcatata aattcctatc ggtaaaattt cccttatcat cccgtggaag aacggccatt
     4621 aatgagtcgt ttttcaggat tgtcggatct ttaagtatca atggatattc aacaggaggt
     4681 atttctactc ggttttctat tttggaataa ttaatttctt taacaaatcc aggcatttcc
     4741 acatggccca gctctctttt taattgttca gatatagaat tgcccatgta ctgcattgat
     4801 tttccaatga aactttcaaa cctgtttaat actccctcca gagcaagcct tctggggtca
     4861 aaagattcct tatcatcaaa aagcttcatg agattgtcgg caacataatt ttcatcattg
     4921 taagccagtg ggaatttggg aaccaaatct ttcggattta ctacactgta gccggtttca
     4981 tttatacaaa atcgcttatt atattctgca gaaaattcct tatcaccaac catgggatgc
     5041 gcgaatgcat aggtcttaaa cttatttttt ggggaaatta ctccttcagg aagattttcc
     5101 aggtaggcac gaagcataat tgccaatgat ccaccctggc tgtgccccgt gattatgaag
     5161 tcataaattc catcacggtt aaaattatca atatgatact tgatgtcatt ggataagtaa
     5221 actatcccca gagcataacc tgcatgaaca gcggcgctgg tatcttttgc aaaactgtaa
     5281 gcaaaagttt caccagccgt tttgatagtc ccgttggcag gtatcatgga agcatacata
     5341 ttctctaacc aactgctcat gtcatcagta gaaccacgca gattaatgac cgcaaccttc
     5401 cctttccggt aaatttgaaa tttgttgtcc atcccaaaaa ttcctgaagt ataaactttc
     5461 tcataatgtg cggggataat tttactgtca gagttataga gctccatgaa cgtgaaacta
     5521 ttgcataaag caatcatatc ccgggcttcc tgcttttcaa atccaggttg aatttgcccg
     5581 aatgaaataa ctgtagttat actaagaatc aggcttatta tgaatttcat gggtgatgat
     5641 tttgaagtca aatataaatc aactattgaa ctcggaagaa atagaaatat agtgttttta
     5701 tggaaattga tattaaaccc aaattgttgc aaaattcaga ccaaaataaa taatacctcc
     5761 gttacaacta tttgtaccaa attccccact aattaataaa tcctgatttc attatgaaaa
     5821 tagccaaaag agtttcttcc gggatgatac tgattttttt cctggcactc accaatcatt
     5881 tatgggccca ggagaacttt ccaaatacgt tattccttgg agaagaagag acctcaccct
     5941 cggcaacaat tgataatatg agttggattg ccggatactg gcaaggggaa gccttcggtg
     6001 gaattgtaga agaattctgg actgcgccga gcgccggaaa catggtttgt gtgtttaaac
     6061 ttatcgtgga gggtaagaca ttgctttatg aattgtgtac aatatctgag gaaaacaata
     6121 cgctgatgat gaggctcaag cattttaaca gtaagcttat aggctgggag gacaaagaca
     6181 aatcaataga ttttcctttg gtgaaaatgg agaagaataa aatttatttt gatgggctga
     6241 cttttgaaag aataag
//
LOCUS       DASWHX010000128        12642 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_108153, whole genome shotgun sequence.
ACCESSION   DASWHX010000128 DASWHX010000000
VERSION     DASWHX010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 12642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12642
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108153"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(61..1077)
                     /gene="pheS"
                     /locus_tag="VGA21_08775"
     CDS             complement(61..1077)
                     /gene="pheS"
                     /locus_tag="VGA21_08775"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009580471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEX9650641.1"
                     /translation="MKEKVEALVREIEEFIATSATEVEQFRLKYISRKGIIPELFQQL
                     KKASAEEKKVLGKHLNELKKSAELKFKALSAGSNTLTSVEADFDITLPPVGGKLGGIH
                     PVSATRDRIIEIFERIGFNTSEGPEIEDDWHNFSALNFPENHPAREMQDTFFIEKNPD
                     IALRTHTSSVQIRVMENNQPPIRTLSLGRVFRNEAISARSHCFFHQCEGLYVDENVSF
                     VDLKQTLLHFAKEMFGKDTITRYRPSYFPFTEPSAEIDISCNICKGEGCPMCKYTGWV
                     EIAGSGMVDPSVLENCGIDSQKYTGFAFGIGLERIAILRHQIGDIRLFSQNDIRFLKQ
                     FRTL"
     gene            complement(1116..2390)
                     /locus_tag="VGA21_08780"
     CDS             complement(1116..2390)
                     /locus_tag="VGA21_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014023055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEX9650642.1"
                     /translation="MSKETKGYLDMELLRFTTAGSVDDGKSTLIGRLLYDSKSIFEDQ
                     YAAIEESSKRKGDTHINLALLTDGLRAEREQGITIDVAYRYFATPKRKFIIADTPGHI
                     QYTRNMVTGASTANLAIILVDARNGVMEQTIRHTFIASLLGIPHIVFCINKMDLVNYD
                     EVIFNKIKDDIEAFSSKLDTKDLRFIPISALNGDNVVNRSENMPWFGGGTLLYLLEDI
                     HIGSDYNHIDVRFPVQYVIRPQNDKYHDYRGYTGRVAGGVLRVNDEVMVLPSGFTSKI
                     KSIGFYDNEFQEAFPPMSVSLTLEDDIDISRGNMIVRINNQPNNGQDIDAMICWLSPK
                     PLLLNGKYGVMHTTNNARCVIKEIVYKIDINTLHRKEDDREIQMNDISRIKIRTTKPL
                     FFDRYTRNRFTGSIILIDEGTHETVAAGMILN"
     gene            complement(2400..3305)
                     /gene="cysD"
                     /locus_tag="VGA21_08785"
     CDS             complement(2400..3305)
                     /gene="cysD"
                     /locus_tag="VGA21_08785"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017731995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HEX9650643.1"
                     /translation="MSNYKLTHLQELESEAIFVIREVAAQFENAAILFSGGKDSIVVS
                     WLARKAFYPARIPFPLLHIDTGHNFKETIDFRDKFVKDLKVELLIGSVQESIDAGKVA
                     EEMGFNASRNALQTVTLLDTMEKYKFDAAIGGARRDEEKARAKERFFSHRDEFGQWDP
                     KNQRPELWNIFNGRKNFGESFRVFPISNWTEMDVWQYIFQEKIGIPSLYFSHERESFV
                     RDGVVMAANEFMQLKPDEKIEKRILRFRTIGDMTCTGATVSKASTLEEIIAEVSASRV
                     TERGTRSDDKRSEAAMEDRKKQGYF"
     gene            complement(3306..3902)
                     /gene="cysC"
                     /locus_tag="VGA21_08790"
     CDS             complement(3306..3902)
                     /gene="cysC"
                     /locus_tag="VGA21_08790"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008200201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HEX9650644.1"
                     /translation="MSTNIYPIFDTLLGKPEKEKLLNQKGIVIWLIGLSGSGKSTIAR
                     ALENQLYKEGNFTQLLDGDNLRTGLNKDLGFSNEDRVENLRRTAETARLFANSGVITI
                     CSFITPTEVSRIQIREILKDLYFEVFVECPLEECERRDVKGIYAKARKGEIKNFTGID
                     APFEDPVNPNLVLKTHKDDLESCLKKLRDSIQSGIKLD"
     gene            complement(3899..4678)
                     /gene="cysQ"
                     /locus_tag="VGA21_08795"
     CDS             complement(3899..4678)
                     /gene="cysQ"
                     /locus_tag="VGA21_08795"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009580466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HEX9650645.1"
                     /translation="MSSMDFKALVDPIFRILQKAGTEIMKVYLDPERSGEIEYKSDNS
                     PLTIADENSNRLIVAELSESFEFPILTEEAKLVDYGERKEWRKFWLVDPLDGTKEFIN
                     RNGEFTVNIALIENGFPVFGAIGVPARDVCYIGYGNTSMKVEGEKKEILRVNGMKSKR
                     IAVRSKSHASPEEEAVLKEYDASGYVSVGSSLKFCMVAEGKADIYYRHGPTMEWDIAA
                     GHAIVRAAGGEVYSGNDTKSVFKYNKENLLNGSFLCLGFKV"
     gene            complement(4697..5596)
                     /locus_tag="VGA21_08800"
     CDS             complement(4697..5596)
                     /locus_tag="VGA21_08800"
                     /inference="COORDINATES: protein motif:HMM:NF013247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HEX9650646.1"
                     /translation="MKKPNIPNPLNENILIYIDGELYSRDEAKISVFDSLAQGGDGVW
                     EGLRVYNGKVFLLDEHLQRLQDSARAMAFSAIPSTEEIKSALFKTLEANKMRDGVHIR
                     LTLSRGKKVTSGMSPHWNQYGSTLIILPEWKPPVFDSKGVRLITSSIRRNPPGCLDSK
                     IHHNNLINNILAKIEANLAGVDDAVMLDLQGFVAETNATNIFFIKKGKVKTPFPDACT
                     PGITRKLIFKLSKELGIPCEEKRISMAEMYSADEAFITGTMGEITPVIDIDGRQISSG
                     EITLKLQNQFYQKTREMGVKLPF"
     gene            complement(5565..6314)
                     /locus_tag="VGA21_08805"
     CDS             complement(5565..6314)
                     /locus_tag="VGA21_08805"
                     /inference="COORDINATES: protein motif:HMM:NF040370.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase family protein"
                     /protein_id="HEX9650647.1"
                     /translation="MKRISMWSGPRNISTAIMYSFAQRPDTQVVDEPLYGYYLSSAEV
                     NHPGREELLNSLETDPEVITKFLIGGDPNKEILFFKNMAHHLVGLDLQFLNNFTNIIL
                     LREPADVINSLSKTLDVVGLRDTGFEFQYRIFEFLNRSGITPIVLDSKDLLKNPGKLL
                     QDLCRLLDIPFYESMLEWKQGGIPEDGSWAKYWYQNVHKSTGFNAYRESNEKLPSHLK
                     DLYEECKSYYDLLTEKKIISYNEKTKYSESP"
     gene            6427..7269
                     /locus_tag="VGA21_08810"
     CDS             6427..7269
                     /locus_tag="VGA21_08810"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX9650648.1"
                     /translation="MKAYKFHVPKRGEEAIRIEVDDLPKFYPIFHYHDELQITLIHQG
                     EGILFAGSGFTNFNQGDIFLLGSNLPHVFKNSSGSKRACSISIYFSPDFAGHGFWNIM
                     ETRSIISVIEDSNKGIKIEGGKNTHLNNQLEKLVSLNPAKKLAGLIDMMDEISQAKRE
                     HISAITYTNPPSGFHTKRMDNIISYIMKNFNQPISLEEISSRANMTSESFCRFFKLRT
                     RKTFTAYLNEVRINNTIKLIMSGDTSVAEAAYISGFRNLSNFNRQFLKITGKRPGEIK
                     VVNS"
     gene            complement(7266..8642)
                     /locus_tag="VGA21_08815"
     CDS             complement(7266..8642)
                     /locus_tag="VGA21_08815"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR03023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HEX9650649.1"
                     /translation="MARYSKYFSPILFLGDLLILNFSFVLGYFIRFGKIEGVWLVPYL
                     YLLIFVNIAWGIVAAVTDPYQIARTARVAKIIRSHLAIIILHLLLVTAFFVFQKAYFY
                     SRLQLVLTYSFFIGIMLIWRVGFIYLLRIYRIRGFNRRNVVVVGFGELTRELQKFFRL
                     HPEHGYKFLGYFDNNEQNSWHLGNFSAMFSYCKQNSVDEIYCCLPYVKYSDIKKIVDF
                     GEDNLIKVKLIADFRGFSFKGLELQRYDHIPVLNVTNIPLDEWKNRVVKRTFDIVFSS
                     LVILLILTWLTPLVGLLVMITSRGPVFFMQKRTGKNNKSFICFKFRTMEVNKDADSLQ
                     ATTKDPRITPIGSFLRKTSIDELPQFFNVLMGDMSVVGPRPHMIKHTKEYSKVVEKFM
                     ARHFVKPGITGLAQAKGYRGETKTVELMKSRVRLDRFYIENWSLVLDLKIIALTIPQL
                     FNGSERAY"
     gene            complement(8716..9981)
                     /locus_tag="VGA21_08820"
     CDS             complement(8716..9981)
                     /locus_tag="VGA21_08820"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEX9650650.1"
                     /translation="MTQVSHKIFAVSLYLVVITILLPRSASNFAIALLILSWLGSAGW
                     QYNNTANFLKRPTSMVSILLLLTYSISFFWTEDLIEGIKNLEVLAPLLIFPVVMAMAK
                     KVEISLENILKVFVYAVISILLINFVIAIFRTLDYGFFFKDPQHTVIYNNFLYHGFAS
                     GTGIHPSFLTLFACFSIMILLNPESMQLGFSRNSKIMMVIILAAGIILLQAITVTFAT
                     IAALSVIFLFKMKRPNIKYSIYLSLVLLLVIVFSYILFIKLDWTRNIVSYNLESYTGW
                     NSVNLRLALWEAAGKAISQNVPWGVGIGDAQHVLLEKFHEMNFHFAAINNYQPHNQFL
                     YSWLMAGLPCVLATILLFVTMVRSAIRKQNHLYLGLVIVFIIFCLTDIPLSRNKPLVF
                     LIFFTYLFEFLDSKGKETGIRASNQKFAK"
     gene            complement(9981..11222)
                     /locus_tag="VGA21_08825"
     CDS             complement(9981..11222)
                     /locus_tag="VGA21_08825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650651.1"
                     /translation="MSILSRFNTYPVHPIQMGLFILFELQLIFGKQFTTFHIVGPLHL
                     SDLVLAILAISGLVLIIVKKITLPLYWPLFGLLGISFIYLIYSFSAALGPLNYIIRHY
                     ALFIYLGLSFIIFYSFADDEKQKINVRFILLMGILALVSQILFHGYNFATVEGYYTQL
                     FSRFNYWTELVMPGLVIIEVWALCFLKKNALKIITALAVLFLSTTLGHASAFLACFVP
                     IIIYFLLIFKIKHWIIITSVIVSMVSLFIFLPQFSDVNAMWRILYWREILKEGFLNYY
                     GVFGHGFGGAFITESMNQTFLNELGSIHFDVNPEEKFLTPMHNSFVTIMFHIGIIPGL
                     LIFVPLKNAFQWAFNYKIEHAASKKFLFLSFMGFVVWASFNVILEMPHSSAFFWFIYF
                     SVLYEFRDPEEKNFSFRIIKD"
     gene            complement(11319..12104)
                     /locus_tag="VGA21_08830"
     CDS             complement(11319..12104)
                     /locus_tag="VGA21_08830"
                     /inference="COORDINATES: protein motif:HMM:TIGR00696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HEX9650652.1"
                     /translation="MITERYKILSLCVNRISYNDALSSLLEKAVNRTAHFFVCFANTH
                     MVVEAYQQKEFAKDLEKASVIFPDGWPVAKAMNVFYGFKQERIAGMDFLTDFLKAGNE
                     RALRIFLFGSTENVIALVRDRIQKNFSNLTVAGHICPSFEDDWDHDAYIEKINEANPN
                     FVLVALGCPKQEKWMAKHFKDVNSLLLGIGAALPVFAGIMPRCPKWLRDNGLEWAYRL
                     VQEPRRLFMRYFTSNFIFVFLFVREFLLIKLGIKRKQNVKEDS"
     gene            complement(12169..>12642)
                     /locus_tag="VGA21_08835"
     CDS             complement(12169..>12642)
                     /locus_tag="VGA21_08835"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX9650653.1"
                     /translation="DFHLRLVGGWPDLVFKKKLLDFYNSVNWDGKVEILGPLYGASKE
                     KVFKGSNILVFPSRYRMECFPLVILEAMSCGLVIMASRVGAVPEILENGELGILVDPN
                     NTQEFASAIIDILDDKERLSRLSNRSMKAFEQKFSFPVFENNFVRILTEILSDIN"
BASE COUNT         3856 a         2687 c         2276 g         3823 t
ORIGIN      
        1 gggcaattcc ctgacccatg gttccggcgc caacaatgcc caccgaatct accaaccttt
       61 tcataaggtt cggaattgct tcagaaaccg gatatcattt tggctgaaaa ggcggatatc
      121 accaatctga tgtctcagaa tagcaattcg ctccaaaccg attccaaagg caaacccggt
      181 atatttttgg gaatcaatgc cgcaattttc cagaacactc ggatccacca ttcctgaacc
      241 ggctatttca acccatcccg tatatttaca catcgggcaa ccttcacctt tgcagatatt
      301 acaagagata tcaatttcag cgctgggttc tgtaaaagga aagtaagagg gacgatacct
      361 ggtaatagta tctttgccaa acatttcttt ggcaaaatgc aaaagggttt gcttcaaatc
      421 aacaaagctc acattttcat ctacatacaa accctcgcac tgatgaaaaa aacaatgtga
      481 acgtgctgag atggcctcat ttctaaatac gcgtcctaat gaaagtgttc ttatcggtgg
      541 ctggttattt tccattaccc ggatttgaac agatgaagta tgagtgcgta acgcaatgtc
      601 cggatttttt tcaatgaaaa aagtgtcttg catttcgcgt gccggatgat tttcaggaaa
      661 attaagcgcg gaaaagttat gccagtcgtc ttcaatttcc ggaccctcac tggtgttaaa
      721 accaattctt tcaaaaattt ctataatgcg atctctggtg gcagataccg gatggatgcc
      781 accaagtttt ccgccgacgg gaggtaaggt aatatcaaaa tctgcttcaa cacttgtcag
      841 cgtattggat ccggcagaca atgctttgaa ctttagttcc gcggatttct taagttcatt
      901 caagtgtttt cccaggactt tcttttcttc cgccgaagcc ttttttagtt gttgaaaaag
      961 ttccggaatt attccttttc tggaaatata cttaagtctg aattgctcaa cttcagtcgc
     1021 actggtggct ataaactcct caatttccct gacaagggct tcaacttttt ccttcatgcc
     1081 tgcaaattaa cagtactagt ttgccaaagc aacacctaat tcaaaatcat tccggccgct
     1141 acggtctcat gggtgccttc atcaatcaga ataatgcttc cggtaaatct gttcctggta
     1201 taccggtcaa agaaaagcgg ctttgttgtt ctgattttta ttctggaaat atcattcatt
     1261 tggatctccc tgtcatcttc ctttcggtgc agtgtattta tgtcaatttt atagactatt
     1321 tctttaatta cacatcgggc attgtttgtg gtatgcataa ctccgtattt accattcagc
     1381 agtaaaggtt tgggacttag ccagcaaatc attgcatcaa tatcctgccc attgttcggc
     1441 tggttattta tgcgcacaat catattgcct ctgctgatat caatgtcgtc ttcaagagta
     1501 agagaaactg acatgggcgg gaatgcctcc tgaaattcat tatcgtaaaa accaatactt
     1561 tttattttag aagtaaatcc tgacggtaaa accatgactt catcattaac tcgcaaaaca
     1621 ccgccggcta ctctaccggt atagccgcgg taatcgtgat acttgtcatt ctgaggacga
     1681 attacgtatt gaaccgggaa tctcacatcg atatggttat agtcactgcc aatgtgaata
     1741 tcttccagca ggtataaaag cgtacctcct ccgaaccacg gcatattttc tgacctgttc
     1801 accacattat ctccattcaa cgcgctgatt ggtatgaatc ttaaatcctt tgtatcaagt
     1861 tttgaagaga aagcttcgat gtcatcctta attttattga atatcacttc atcataattt
     1921 accagatcca ttttgttgat gcaaaaaaca atatgtggta ttcccagaag tgaagcgatg
     1981 aatgtatgcc tgattgtttg ctccattaca ccattccgtg catcaacgag tattattgcc
     2041 aggttggccg tgcttgcacc tgttaccata ttccgggtat attgaatatg tccgggggta
     2101 tcagcaataa taaactttcg tttgggtgtg gcaaagtacc ggtatgcaac gtcaatagta
     2161 attccttgct ccctttcggc gcgaagtccg tctgtcagca aagcgaggtt aatgtgggtg
     2221 tcgcctttcc gcttgcttga ttcttctatc gccgcatatt ggtcttcaaa aatcgatttt
     2281 gaatcataga gcaatctgcc aattagcgta cttttaccat catctacact tcccgcggtt
     2341 gtaaaccgca acaactccat atccagataa ccttttgttt ctttactcat ttcgtatcat
     2401 taaaagtagc cttgcttttt tctatcctcc attgcagctt ctgagcgttt atcatcagag
     2461 cgggttcccc gctcagtcac tctcgatgca gaaacttcag caataatttc ttccaaagtt
     2521 gatgctttgg aaactgtggc acccgtgcag gtcatgtctc cgattgttct gaaccttaag
     2581 attctttttt caattttttc atcaggtttc agttgcataa attcattggc agccatcacc
     2641 actccatctc gcacaaagga ttccctttca tgtgaaaaat acagtgaggg gattccaatc
     2701 ttctcctgga aaatatattg ccaaacatcc atttccgtcc agttgcttat tggaaaaacc
     2761 cggaaacttt cgccgaaatt ttttctccca ttgaagatat tccataattc cgggcgttga
     2821 ttctttggat cccactggcc aaactcatcc ctatggctga aaaacctttc tttagcgcgt
     2881 gctttttctt catctctgcg tgcgcctcca atcgcggcat caaatttata cttctccata
     2941 gtatccaaaa gagtcaccgt ttgtaatgcg tttcttgatg cattaaaacc catttcttcc
     3001 gcaacctttc ctgcatcaat tgactcttgc actgagccaa tcaacaattc aacctttaaa
     3061 tcctttacaa acttatctct aaaatcaata gtttctttaa agttatggcc agtgtcaata
     3121 tgaagcagtg gaaagggaat tcttgcgggg taaaaagctt ttcttgccaa ccagcttacc
     3181 acaattgaat ctttgcctcc ggagaaaagt atggctgcat tttcaaactg agcagcgact
     3241 tcacgaatca cgaatattgc ttcagactcc agttcctgta aatgggttaa tttataatta
     3301 ctcatttaat ctaatttaat tccagactga atactgtctc ttaatttttt cagacaactt
     3361 tccaaatcat ctttatgtgt tttcaaaacc aggtttgggt taaccggatc ttcaaatgga
     3421 gcatcaattc cggtgaaatt tttgatttcc cctttccggg ctttcgcgta aattccttta
     3481 acatctcttc gttcacattc ctcaagcggg cactctacga acacttcgaa atataaatcc
     3541 ttgagaattt ccctaatttg aattcttgaa acctcggttg gagttatgaa ggaacaaata
     3601 gtaatcacac ctgaatttgc aaataacctg gccgtctcgg cagttcgccg caaattctca
     3661 acgcgatctt cattggaaaa tcccaaatcc ttgttcagac cggtgcgtaa attatctcca
     3721 tcaagcaact gggtaaaatt accttctttg taaagttgat tttccagggc acgggcgatc
     3781 gtacttttac cagaacccga caaacctatt agccagatta caattccctt ctggttcaga
     3841 agtttttcct tctcaggttt tcccagcagg gtatcgaata tcggataaat atttgtactc
     3901 atactttaaa tcccaaacat aggaacgaac catttaataa attctcctta ttatacttga
     3961 aaacagactt ggtatcattt ccggaataga cctcaccacc ggcggcgcga acgatggcat
     4021 gcccggctgc aatatcccat tccatagtag gaccatgtct gtaatagata tccgcttttc
     4081 cctcggccac catacagaat tttaatgaac ttccaaccga tacataaccg gaagcatcat
     4141 attctttaag caccgcttct tcttcggggg acgcatgact cttacttcta actgcaattc
     4201 tttttgactt cattccatta acccttaaga tttctttctt ctcaccctcc actttcattg
     4261 aagtattccc atatccaata tagcagacgt ctcttgcggg aacaccgatt gcaccaaaaa
     4321 ccggaaatcc attttcaatt aaagcaatgt taaccgtgaa ttcgccattt ctgttaatga
     4381 attctttagt cccgtccagc ggatctacca gccagaattt tctccattcc tttcgctctc
     4441 cataatcaac aagtttagct tcctctgtta aaattggaaa ttcaaaactt tcagaaagtt
     4501 cagctacaat taaccggtta gagttttcgt cggcaattgt caggggtgag ttatcggatt
     4561 tatattctat ttcaccgcta cgttcaggat ccaaataaac tttcattatt tcagttcctg
     4621 ctttttgtaa tattctaaat attggatcga ctaacgcttt aaaatccatg ctgctcattg
     4681 gaggtgcaaa tttcgtctaa aaaggcaact ttacgcccat ttctctggtc ttttgataaa
     4741 attgattttg cagtttcaag gtaatttctc cggaagaaat ttgacgtcca tcaatatcaa
     4801 taaccggggt tatttcaccc atcgtacctg taataaaagc ttcgtccgcg ctgtacattt
     4861 ctgccatgct aattcgcttt tcttcacagg gaatacctaa ttcttttgaa agtttgaata
     4921 ttaattttcg ggtaattcca ggggtacatg catccggaaa tggtgttttc actttacctt
     4981 ttttaatgaa aaaaatattg gtagcattcg tctcagcaac aaatccctgc aaatccagca
     5041 ttaccgcatc atccacgccg gctagatttg cttcaatttt agccagaata ttatttatta
     5101 aattattgtg gtggattttg gaatccagac agcccggcgg atttcttcta atggaagaag
     5161 ttattaatct tacacctttg gaatcaaaga ccgggggttt ccattccggt agaattatta
     5221 aagtggagcc atattgattc cagtgcgggc tcattcctga agttaccttt ttacccctgc
     5281 tgagggttaa gcgtatatgt acgccatccc gcattttatt agcctccaga gttttgaaaa
     5341 gtgcagattt tatttcttcc gttgaaggaa ttgcagaaaa tgccatagcc cgggctgaat
     5401 cctgtaatcg ctgaagatgc tcatccagca aaaatacttt tccgttataa accctcaatc
     5461 cctcccaaac tccatcgcca ccctgggcaa ggctgtcgaa gactgaaatt ttagcttcat
     5521 ccctggagta gagttctcca tctatgtaga ttaatatgtt ctcattaagg ggattcggaa
     5581 tatttggttt tttcattata actaattatt tttttttcag ttagcagatc ataatacgat
     5641 ttgcactctt catataaatc tttcagatga gaagggagtt tttcatttga ttcccgatat
     5701 gcattaaaac ctgtcgattt gtgaacattt tgataccagt atttagccca ggaaccatct
     5761 tcaggaattc ctccttgctt ccattccagc atagattcat aaaaaggaat atccaataat
     5821 cgacaaagat cctgcaacag ctttccggga tttttcagca ggtcttttga gtcgagtaca
     5881 ataggcgtaa ttccactcct atttaagaac tcaaatattc tgtattgaaa ctcaaagccg
     5941 gtatctcgta atccaaccac atccagggtt ttggatagag aattaatgac atcagccggt
     6001 tctcttagca ggataatgtt tgtaaagttg tttaagaatt gaagatccag cccgacaagg
     6061 tggtgagcca tgttcttgaa aaataaaatc tccttatttg gatctcctcc gatcaggaac
     6121 ttggtaatta cctccggatc agtctcaagg ctgtttagca gttcctctct tccaggatga
     6181 ttgacctctg ctgatgataa ataataccca tacagcggct catccaccac ctgcgtatcg
     6241 ggtctttggg caaatgaata catgatggcc gtacttatat tccttggtcc agaccacatt
     6301 gagattcttt tcattattca aagataatca cattagggaa tgcccggagg tattaattta
     6361 gccatttatg atactttttt gacatataat cccttatatt caaaataaaa ttggtaatca
     6421 agcatcatga aggcttataa attccatgtt ccaaaacggg gagaagaagc catccggatt
     6481 gaagttgatg acctgccaaa attttacccg atttttcatt atcatgatga attacaaatt
     6541 accttaattc accaggggga aggaatttta tttgccggga gtggttttac aaatttcaat
     6601 caaggggata tctttctctt gggttctaac cttcctcatg ttttcaagaa ttcatccggg
     6661 tcaaaaagag cgtgttccat ttccatctat ttctcaccgg actttgccgg gcatgggttc
     6721 tggaacataa tggaaaccag gtccattata tcggtaattg aggattccaa taagggcatt
     6781 aagatagagg gtggtaaaaa cacccatttg aataaccagc ttgaaaaact agtaagtctg
     6841 aatcctgcaa aaaaattagc cggattaata gatatgatgg atgaaattag ccaagcaaaa
     6901 agagaacaca tctcagctat tacttacacc aatccccctt ccggtttcca tacgaaaaga
     6961 atggataata ttattagcta cattatgaag aacttcaatc aaccaattag tctggaagaa
     7021 atatcttcaa gggcaaacat gacctcagaa tctttttgcc ggttttttaa attgagaacc
     7081 cgtaaaacgt tcaccgccta tttgaatgaa gtaagaatta ataataccat aaagctgatc
     7141 atgagtggag atacgtctgt ggcggaggct gcctacataa gtggtttcag gaacctttct
     7201 aattttaacc gccagttttt gaagattacg ggaaaaaggc ctggagaaat aaaggttgtt
     7261 aacagttaat aagctctctc tgatccatta aataattgcg gaattgtcag tgctattatt
     7321 ttcaaatcca gaactaaaga ccagttttca atatagaacc tatcaagacg taccctgctt
     7381 ttcattaatt ccacggtctt agtttcgcca cgatacccct ttgcctgagc caatccggta
     7441 atgccgggtt taacgaagtg cctggccatg aatttttcca cgacttttga atattctttg
     7501 gtgtgtttta tcatgtgagg ccttggtcca accacagaca tgtcacccat cagaacatta
     7561 aaaaactgtg gcaattcgtc aatactggtt tttcttagga acgatcctat cggggtaatc
     7621 cgggggtcct tcgttgttgc ctgaagggaa tcagcatcct tattcacttc catggttcta
     7681 aacttaaagc agataaagct tttgttattt ttaccggtgc gcttctgcat gaaaaaaacg
     7741 ggacctctgg acgttatcat tacaaggagc ccaaccaggg gagtaagcca ggtaagtatt
     7801 aagagaatta ccaatgagga aaatacgata tcaaaagttc ttttaactac cctgttcttc
     7861 cattcatcca gtggaatatt tgtaacattc agaaccggga tgtgatcata tcgctggagt
     7921 tcgagaccct tgaacgaaaa gcctctgaaa tccgcgatca attttacttt aatcaaattg
     7981 tcttcaccaa agtccacaat tttcttgatg tcactgtatt ttacataagg gaggcagcaa
     8041 taaatttcgt ctacagaatt ctgtttgcaa taggagaaca ttgcagagaa atttcccaga
     8101 tgccaggagt tctgttcatt attatcaaaa tagcccagaa acttgtaccc gtgttcagga
     8161 tgcaatctga agaatttctg caattcccgg gtcaactcac caaaaccaac aactacaaca
     8221 tttcttctat taaaacctct gatcctgtat attctgagga ggtaaatgaa cccaactctc
     8281 caaatcagca taattcctat aaaaaaagaa taagtcagaa ccaattggag ccgtgaatag
     8341 aaataggcct tttgaaatac gaaaaaggcg gttacaagta acaggtgcaa aataatgatg
     8401 gccaggtgac ttcttatgat cttggcaact ctcgcagttc ttgcaatctg gtagggatcg
     8461 gtaacggcag ccacaattcc ccaggcaata tttacaaaga taagcaggta taaatagggc
     8521 actagccaaa cgccctctat tttgccaaat cgaatgaaat agcctaaaac aaaggaaaaa
     8581 ttaaggatta gcaagtctcc aagaaaaagt atgggactga aatactttga gtaacgggcc
     8641 attctcaggg aaatttgggc ttcaaaaata actcctaaat cgtttgaaac ccagtaaaaa
     8701 gggctgaaaa attggttatt tggcaaattt ttgattggaa gccctaatac ctgtctcttt
     8761 accttttgag tctagaaatt caaacaaata ggtgaagaat atcaggaata caagtggttt
     8821 gttccttgaa agcgggatat cggtgaggca aaaaattatg aagacaatca ctaggcccaa
     8881 ataaaggtga ttttgctttc ttatcgccga tctaaccatt gtcacaaaaa gaagaatagt
     8941 tgcaaggaca caaggcagtc ctgccatgag ccaggagtaa agaaattgat tatgcggttg
     9001 ataattattg atggcagcga agtggaaatt catttcatga aacttttcca ataaaacgtg
     9061 ttgggcatca ccaatgccaa ctccccatgg tacattttgt gagatagcct taccggcagc
     9121 ttcccaaagg gcgagcctta aattaaccga attccaaccc gtatacgatt caaggttata
     9181 tgaaactatg tttctggtcc aatctaattt tataaacagt atgtatgaaa atactataac
     9241 cagcaaaagc actagcgaca ggtaaatgct atatttaatg ttcggccttt tcattttgaa
     9301 aagaaaaatt acactcagcg cagcgatggt agcgaatgtt actgtgatgg cttgaagcaa
     9361 aataatgcct gccgccaaaa ttattaccat cataatttta gaatttcggc taaatcccaa
     9421 ttgcatgctt tcagggttaa gcagaatcat aatggagaag caggcgaaaa gagtcaaaaa
     9481 gctgggatgt attcctgtcc ctgaagcaaa accatgataa agaaaattat tgtaaatgac
     9541 cgtgtgctga ggatccttga aaaagaaacc gtaatcaagg gtacgaaaaa tggcaattac
     9601 aaagttgatt agtaaaatgg agataacagc ataaacaaaa actttcaaaa tattctcgag
     9661 ggagatttct acttttttag ccatcgccat cacaaccggg aaaatcagca gcggtgccag
     9721 cacttccagg tttttaattc cttcaatgag atcttctgtc caaaaaaaac ttatggagta
     9781 tgtcaaaagc aacagtattg ataccatgga ggttggtctt ttcaggaaat ttgcggtgtt
     9841 gttatattgc catcctgctg aacccaacca ggaaagtatt aataatgcta tggcaaaatt
     9901 actagcggat ctcggcagta atatggtgat tacaaccagg tataaactaa cagcaaatat
     9961 tttatggctg acctgtgtca ttaatctttt atgattctga aactaaagtt tttttcctca
    10021 ggatctctga attcataaag aacactgaaa tatataaacc agaagaaagc cgaactatgt
    10081 ggcatttcaa gaatcacatt gaaagaagcc cacaccacaa aacccatgaa gcttagaaaa
    10141 aggaattttt tagaagccgc atgttcaatt ttgtaattga atgcccattg aaaagcattt
    10201 ttcaaaggaa caaatatcaa aagccccggt attattccaa tgtgaaacat gatagtaacg
    10261 aatgagttat gcattggagt gaggaatttc tcttcagggt tcacatcaaa atggattgaa
    10321 cctaattcat ttaaaaaagt ctggttcatg gattctgtaa taaacgctcc accgaatcca
    10381 tggccgaaaa ctccgtaata gtttagaaat ccttccttca aaatttcgcg ccaatacaat
    10441 attctccaca ttgcatttac atcgctgaat tgaggcagga agataaataa ggagaccatt
    10501 gatacaataa ccgatgttat aataatccag tgtttaattt taaaaattaa gaggaaataa
    10561 ataatgattg gaacaaagca agcgaggaat gcagaggcgt gtccgagagt agtgctcaaa
    10621 aacaaaactg ctaatgccgt aataatcttt agagcattct ttttcaaaaa gcaaagcgcc
    10681 caaacttcga ttatcaccag accaggcatt accaattcag tccaataatt gaatcttgaa
    10741 aaaagctggg tataatatcc ctctaccgtt gcaaaattat atccatgaaa cagaatctgg
    10801 ctaacaagag caaggatacc cattaaaagg ataaacctta cattgatttt ctgtttttcg
    10861 tcgtcagcaa aactatagaa tattataaac gaaagcccca ggtaaatgaa caatgcataa
    10921 tgccttatta tataattcaa aggccctaat gctgctgaaa aagaataaat caggtaaata
    10981 aagctgatac ctaacaatcc aaacaaaggc cagtaaagag gcaatgttat tttcttaact
    11041 ataatcaaaa caagaccgga aattgctaaa attgcaagca ctaaatcact caaatgcaat
    11101 ggtcccacta tatgaaaggt ggtgaactgc tttccaaaaa tcaactgtaa ttcaaacaaa
    11161 ataaaaagcc ccatttggat gggatgaacg ggatacgtat taaaccggct taatatgctc
    11221 atcttatgtg aatcagcttt atttgggcaa cttcaagaga gtcaaatata aaacaagtat
    11281 aaaagttttg agactttatc ctggctggaa tacccttttt aggaatcttc cttaacattc
    11341 tgtttccttt tgatccctaa ttttattaat aagaattcgc gtacaaataa aaagacaaag
    11401 atgaagttag aggtaaaata ccgcataaat aatctgcgcg gctcctggac cagacggtat
    11461 gcccactcaa gcccgttatc tcgtagccat tttgggcacc ttggcattat accagcaaat
    11521 acaggcaatg cggccccaat gcccagcagc aatgaattta catccttgaa gtgttttgcc
    11581 atccactttt cctgttttgg acaaccaagt gcaacaagaa cgaagttcgg atttgcttca
    11641 tttatctttt caatatacgc atcgtgatcc cagtcgtctt cgaatgacgg acagatatgc
    11701 ccggcaacag tcagattaga gaaattcttt tgaattcgat ctctaaccag ggcaattaca
    11761 ttttccgtgg aaccaaaaag aaatattctt agcgctcttt cattgcctgc tttcagaaaa
    11821 tctgtgagaa aatccatgcc cgcaattctc tcctgtttaa agccatagaa tacattcatg
    11881 gctttcgcga ccggccagcc atccggaaaa atgacggatg ctttttccag gtctttagca
    11941 aattctttct gttgatatgc ttccaccacc atgtgagtat tggcaaaaca aacaaaaaag
    12001 tgggcggtgc ggttaacggc tttctcaagc aggctgctga gagcatcgtt gtaagaaatt
    12061 ctgtttacac ataggctcaa aatcttgtat ctctctgtta tcaaaatagt agaaggtaaa
    12121 tttttctggt cataaatata ggcagtaaac cttgctgatt tagatacttt aattaatatc
    12181 agaaagaatt tccgttagta tcctcacaaa attattctca aaaaccggaa atgaaaattt
    12241 ttgctcaaac gctttcattg accggttgct caatctactt aatcgttctt tatcatctaa
    12301 aatatcaata attgctgagg caaattcctg agtgttattg gggtcaacca aaattcctaa
    12361 ttctccgttt tccagtattt ctggtactgc accaactctg cttgccatta taaccagacc
    12421 gcaactcatt gcttccaata ttactaaagg aaaacattcc attctatacc ttgagggaaa
    12481 caccaatata tttgagcctt taaatacttt ttccttagat gccccatata aggggccgag
    12541 aatttcaact tttccatccc aattcactga attatagaag tctaacagct tctttttaaa
    12601 taccaaatcg ggccagcctc ccaccagacg taagtgaaaa tc
//
LOCUS       DASWHX010000129         4138 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_3089029, whole genome shotgun sequence.
ACCESSION   DASWHX010000129 DASWHX010000000
VERSION     DASWHX010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 4138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4138
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3089029"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            259..1329
                     /locus_tag="VGA21_08840"
     CDS             259..1329
                     /locus_tag="VGA21_08840"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEX9650654.1"
                     /translation="MNFSCIIVLLCLIPTITHAQEVKYDKVGLRRLAVYLPEGYNEST
                     QNFKTVYFYDGQASFGNHPYSWKIHEKLTSLISEGKIEKVVAVGIYTDVNRTEDLVPY
                     YDPYIAENWGSYKPKGDKFDDFIIESLIPYIESKYKVQTESSSRALIGMSFAGLHAMY
                     TGINHQKSFALVAGISASLWVDNYRFIKELKKVNIGPKIWFDIGTAEWNSYTDAIDLL
                     LDAGYEYGESLFYLEESGAAHEAKYWIPRIEYPLILFAGATDNRISTFEIKIEVIPSA
                     TSKKHYLRINPIVTREDGVKYSLATQASYEVMNPGSGQVNEDGSFEFMNNSDMKVRVS
                     FQDWKEEIIILYSDIRARMKKD"
     gene            1339..1791
                     /locus_tag="VGA21_08845"
     CDS             1339..1791
                     /locus_tag="VGA21_08845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650655.1"
                     /translation="MKEKILLGIQKVFFPLELVALILISLKFFLEGDNQITTGVGFIL
                     LAALYFFTAYFSLQPGISRFAGFSKKILGVALSVSVIGIYFTFMHLPGAQTMLTIGVT
                     SIIISMIFLLIEILRKSETANLVKFSVIRGLVISICSALLLYFADFNA"
     gene            complement(1781..3628)
                     /locus_tag="VGA21_08850"
     CDS             complement(1781..3628)
                     /locus_tag="VGA21_08850"
                     /inference="COORDINATES: protein motif:HMM:NF036516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M61 family peptidase"
                     /protein_id="HEX9650656.1"
                     /translation="MKKILLLFIVCFQFSCYEYSNKRSASNDGEPVVIKLDLSEIEHH
                     ELRISYTITGVPFDTLFMRMPQSSPGRYALHQFAKNVYSEAAGDGNGKAISWVKTDVS
                     EWAIFGHDGTVKFEYTLYANRAGGTYTGVDNRKVHLNMPATFMYPVELDKRKVELEFD
                     LSNQGEWTVATQLEKIDDTHFMASDFYYFYDSPTIAGNIDWRTWEVTSNSKTQSIRIA
                     MMHEGTDEELDDYTEWVKKVVAESEIIFGELPEFDYGEYTFLTMYNPWVFGDGMEHRN
                     STICSSRGNLANNAGGLIGTISHEFFHCWNVERIRPETLEPFNFDKPNLSGELWFAEG
                     FTSYYDGLILKRAGIQDSVSFVSSLAGTLNYVINSPGRRFKNIIEMSRHAPFVDAATA
                     IDETNFSNIFTSYYSYGSAIGLALDLTIRAQFPGKSLDDLMKYMWQNYGKVEKPYTIE
                     NIEFALAETTGNPEFSGQFFDNFIYKSNFPDMMEILQPFGIEINLRNPGKAGFPGLQL
                     SYSPNGAEVQSPILTTNPLYEAGVTNGDVILELNGKPIVDEGDFFINYELGNSYLIKY
                     IQRGETKERTFIPTEDQSFTTSFIEASGGKPTANQLQNRRNWLETKVRH"
     gene            complement(3635..>4138)
                     /locus_tag="VGA21_08855"
     CDS             complement(3635..>4138)
                     /locus_tag="VGA21_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008199177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX9650657.1"
                     /translation="NKVLAEMMHENLSEVGGVIYDEKEMEFARKIIATYGYEVDESII
                     EESDDIAPFEVRTRGSGGSTDVGDISWVVPTAGLRAATWVPGTSAHSWQAVAAGGMSI
                     GTKGMMVAAKTISLTAMDIFQNPDKIKEAKAELLERRGNNFKYQPLLGDRKPPLDYRL
                     NTRPEEK"
BASE COUNT         1215 a          852 c          765 g         1306 t
ORIGIN      
        1 caatcctttt gcatttggtg ttatacttac ttgtttcatt tcccggtatg ttcattcacc
       61 gaaacccact ttcggaaatt ggcggtctga tttatttttt aatgccttca gggattactt
      121 tggcattgat tatttcatat cccctggtac tggttatccg gaaaatcaat tcgaattaga
      181 attccaaacg aaggtttaat gtagaattaa ttgatgattc attttttttg ttgcaaattg
      241 ttgagataaa cccctttgat gaattttagc tgcattatag tattactttg cttaatcccc
      301 actattacac atgctcaaga ggtaaaatat gacaaagttg gattaagaag actggctgtt
      361 tacctgccag agggatataa tgaatctacg cagaacttta agacagtgta tttctacgat
      421 ggtcaggcat cattcggaaa tcacccttat tcatggaaga ttcatgaaaa attaacaagt
      481 ttgatatccg aaggtaagat tgaaaaagtt gtggcagtag gtatttatac agatgttaac
      541 cggacagagg atctcgtccc ttattatgac ccttacatcg ctgaaaactg gggctcatat
      601 aaacccaaag gagataaatt tgatgatttt attattgagt ctttaattcc ctatattgaa
      661 agtaaataca aagttcaaac tgaaagcagc agccgggcat taatcggaat gtctttcgcg
      721 gggctccatg caatgtatac aggcattaac catcaaaagt catttgccct ggttgcagga
      781 atttccgcat cactttgggt agataactat cgctttatta aagaattaaa gaaagtaaat
      841 attggaccga aaatatggtt tgatattggt accgcggaat ggaatagcta cacagacgcc
      901 attgaccttt tacttgatgc cgggtatgag tacggtgaaa gtctgtttta tcttgaagaa
      961 agtggtgcgg cccatgaagc gaagtattgg attccccgca ttgaatatcc cctgattctg
     1021 tttgcaggtg ctaccgataa tcgaatctca acttttgaaa ttaaaataga agttataccc
     1081 agtgctactt caaagaagca ctacttacga ataaatccca ttgtcacacg ggaagacgga
     1141 gtaaaatact cattagcaac tcaagccagc tacgaagtaa tgaaccccgg cagtggacaa
     1201 gttaatgaag acggaagctt tgaatttatg aataactctg atatgaaggt tagggtatca
     1261 ttccaggatt ggaaagaaga aataattatt ttatactcag acattcgagc cagaatgaaa
     1321 aaagattaat attacagcat gaaagaaaaa atcctgcttg gcattcaaaa ggttttcttc
     1381 cctttggagc ttgttgcatt aatactgatc tcacttaaat tctttcttga gggagacaat
     1441 cagataacaa ctggagttgg tttcattttg ctggcagctc tttatttttt cacagcatat
     1501 ttttctctgc agcccgggat aagcagattt gccgggtttt caaagaaaat tctgggtgtt
     1561 gctcttagtg tttcagtaat tggaatctat tttaccttta tgcatttgcc gggagctcaa
     1621 accatgttaa caattggtgt gacttcaata atcatctcaa tgatttttct attgatagaa
     1681 attcttcgaa aatcagagac tgccaatctg gttaagtttt ccgttatcag aggcttggta
     1741 atttcaatct gcagtgccct tttattatac tttgcggatt tcaatgcctg actttagttt
     1801 cgagccagtt ccttcgattt tgcaattggt ttgctgttgg ctttccacca gatgcttcaa
     1861 taaatgaagt agtaaatgat tggtcctctg ttggaataaa agttcgctct ttagtttctc
     1921 cgcgttggat atactttatc aaatatgaat tgcccaactc ataattgata aagaaatctc
     1981 cttcgtcaac aatgggcttg ccattcaatt ccaaaattac atctccattg gtcactcctg
     2041 cttcgtacag tggattggtg gtcagaatcg gagattgaac ttcagcgcca ttcggtgaat
     2101 aactaagttg caaacctgga aacccggcct taccgggatt ccgcaaatta atttctatac
     2161 caaagggttg gagtatttcc atcatatcgg ggaaattact cttataaata aagttgtcaa
     2221 aaaattggcc ggaaaactca ggatttccgg ttgtttcggc cagtgcaaat tcaatatttt
     2281 caatagtata aggtttctcc actttaccat aattttgcca catatatttc atcaaatcat
     2341 caagcgactt tccggggaat tgagcgcgaa tagttagatc cagcgcaagg cctattgcgg
     2401 aaccgtaact gtaatagctg gtaaagatat tgctaaaatt agtttcatca atggcagtgg
     2461 cagcatctac aaaaggtgca tgtcggctca tttctataat atttttaaat cttctgccag
     2521 ggctattgat cacataatta agcgttccgg ccagactact cacaaaactt actgaatctt
     2581 gtatgcctgc tcttttcagg atcaaaccat cataataaga agtgaatcct tccgcgaacc
     2641 ataattcgcc tgacaggttt ggcttgtcaa aattgaaagg ttcaagagtt tctggcctga
     2701 ttctttccac attccagcaa tgaaaaaatt cgtgagaaat agttccaatc aatccacctg
     2761 cattatttgc gaggttgcca cggcttgagc aaatcgtact attacgatgc tccattccat
     2821 ctccaaaaac ccatggatta tacatagtaa ggaacgtata ttcaccataa tcaaattctg
     2881 gcaattcacc aaaaataatt tctgactccg cgactacctt tttaacccac tccgtataat
     2941 catctaattc ttcatctgtt ccttcatgca tcattgcaat ccggatagat tgagttttgg
     3001 agttggaggt tacctcccag gtacgccaat caatattgcc ggcgattgtc ggactgtcat
     3061 agaaataata aaaatcagaa gccataaaat gagtatcatc aatcttctcc aattgagttg
     3121 ctactgtcca ctcaccttga tttgaaagat caaactccaa ttcaactttc cttttatcca
     3181 attcaaccgg atacataaaa gtggcgggca tattcaaatg aactttccta ttatcaacac
     3241 ctgtataagt accccctgcc ctgtttgcat acagagtgta ttcaaatttc acggttccat
     3301 catgtccgaa tattgcccac tcgctgacat ctgttttcac ccacgaaata gcttttccat
     3361 tgccatcgcc ggccgcttcg gaataaacat tctttgcaaa ctgatgcagc gcatatcttc
     3421 ccggagatga ttggggcatt ctcataaaaa gggtatcaaa gggaacgccg gtaatagtat
     3481 agctgattct taattcatga tgttctattt cagataagtc aagcttaata actactggtt
     3541 ctccatcatt ggaggcactt ctcttatttg aatattcata gcacgaaaat tgaaagcaga
     3601 caataaataa caagagtatt tttttcattg atagttattt ttcttcggga cgagtattca
     3661 gtctgtaatc gagaggaggt tttctgtctc ccaataaagg ttgatactta aagttattgc
     3721 ctcttcgctc cagaagttct gcttttgctt cctttatttt atcaggattc tgaaaaatat
     3781 ccattgccgt cagggaaatc gttttggcgg caaccatcat tccttttgtt ccgattgaca
     3841 taccacctgc agccaccgcc tgccagctat gcgctgaagt tcccggaacc caggttgcag
     3901 cacgtaaccc tgcagtggga accacccaac taatatctcc gacatcagtt gagccgccgg
     3961 agccgcgagt tctaacctca aaaggagcta tgtcatcact ttcttcaatt atagattcat
     4021 ctacttcata cccgtaagta gcaatgatct tccgggcaaa ttccatttct ttctcatcat
     4081 aaattactcc gccaacctcg ctaagatttt catgcatcat ttctgcaaga accttatt
//
LOCUS       DASWHX010000130        28808 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_852922, whole genome shotgun sequence.
ACCESSION   DASWHX010000130 DASWHX010000000
VERSION     DASWHX010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 28808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28808
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_852922"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            132..965
                     /locus_tag="VGA21_08860"
     CDS             132..965
                     /locus_tag="VGA21_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020527166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HEX9650658.1"
                     /translation="MEVDLKNLSAYTDEAIKLALAYGPKLVLAILTLIVGLWVIKWFS
                     KGMIKVMDSRRLDPSLKPFFSSLISITLKILLAISVMGMIGIEMTSFIALLGAAGLAF
                     GMALSGTLQNFAGGVVLLVFKPFKVGDFVEAAGYSGTVSEIRIFNTILKTPDNKTIII
                     PNNAIAGSSMVNYTTEATRRVDFVFGISYQDDIDLTKSILNRLIERDERIHKDPAPFI
                     GLTEMADSSVNLVVRVWADTPNYWPIYFDMMEDVKKTFDAEGISIPFPQRDVHLYNQK
                     A"
     gene            969..1901
                     /locus_tag="VGA21_08865"
     CDS             969..1901
                     /locus_tag="VGA21_08865"
                     /inference="COORDINATES: protein motif:HMM:NF022720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3078 domain-containing protein"
                     /protein_id="HEX9650659.1"
                     /translation="MIRRILLFMLVPVSFLAKSQEDTTYWRAGLSSTIAFSQLSLTNW
                     ASGGQNSIALNSGLTLFADYAKEKISWKNKIDLAYGFIQQKDDGYRKSDDRIIVITEF
                     GYLISRKPNRMKWTTMIDFKTQFDKGYNYPNDSMKISDFMAPGYLTIVTGLEYSPKPH
                     LTVLYSPVSGKFTFVNDQRLSNAGGFGVEGAVYDIDGILIQDGKNLNSEFGSYMRVQF
                     EKEVMTNVTVFSKAEFFTDYLMDFGIIDVNWESKLDMKINNWLSANLILHLIYDRDIR
                     FEVTDANGIITGTEDRIQFKEYLGVGFKYNVSNR"
     gene            1981..3177
                     /locus_tag="VGA21_08870"
     CDS             1981..3177
                     /locus_tag="VGA21_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019947618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlmU family protein"
                     /protein_id="HEX9650660.1"
                     /translation="MNVILFDYNPWRDSLQPLTYTRPVSELRVGILTIKEKWESELSV
                     RISYLTRNYLSEKFPLSISDENLLINGSWCPMAGVKDLLQLNTGESLVWQGIPLALKV
                     SKDGFSKIHNQDFKTKKEIQCEKVPVLISKLWHLFKNNGSEIRIDFERLTKGRKSEPL
                     TDPGCTIYGKENIFLESGAMVRAAILNAEDGPIYLGKNSRVQEGSIIKGPFAMGEGSH
                     INMGAKVKGDSTLGPFCKVGGEISNSILLGYSNKAHDGFLGNSVIGEWCNIGADTNNS
                     NLKNNYDEVKLWDFSRQRFELTGETFVGLFMGDHCKCAINTMFNTATVTGVASNIFGA
                     GFPRTFIPSFSWGGPAGFITFKLEKVFEMAGAMMNRRGVQLTEVDKKILTKVFELSVE
                     YRTWER"
     gene            complement(3209..3487)
                     /locus_tag="VGA21_08875"
     CDS             complement(3209..3487)
                     /locus_tag="VGA21_08875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650661.1"
                     /translation="MSVLDSKDTHRNLKKKGFIDSPGDHKRLEYYYNDKLILHTKISH
                     GSKKDLDDYLIRQMSVQCQLDKMQFVDLAKCPLSKGEYLKILRKKKLL"
     gene            complement(3487..4407)
                     /locus_tag="VGA21_08880"
     CDS             complement(3487..4407)
                     /locus_tag="VGA21_08880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650662.1"
                     /translation="MKINLNKGVSFLIEGEVGRFHTLPVDTLIKIAENLQNLLITIAK
                     CDIDSKEPIHLENFKVELSGFRHGSAIPEFVFSPRLQTSIGNVELQRRVVNKRFENLM
                     KLSNKSNYFKINEIYPDSYKRNEIVDVLYDFTNSFDDAPTSIVGVDQKKILKPIYRIK
                     RLKPEVKKKLTTKIKDLAVDRLEEYAVARVKLITTKKGTKSKIQEIYKKSYTTLSYSP
                     QIIAYGQLVYNLNFPLRSSLEKENDYYVIHNEMLDIIGTGQSEEEAEQNFAEEFDYIY
                     KRYNELDNNSLSKKLNRIKVFLNHLIKSVE"
     gene            complement(4462..5619)
                     /locus_tag="VGA21_08885"
     CDS             complement(4462..5619)
                     /locus_tag="VGA21_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010611784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HEX9650663.1"
                     /translation="MTYYHKLGEIPPKRHTQFRQPDGSLYKEELVSSKGFSGIYSNLY
                     HIEAPTRIKEIQDPLPYGPKRIETVMKPTHLNTSEAGITGNDYLDARKVMLVNNDVSL
                     ALCIPKEKSMDYYYKNAEGDEVIFIHDGNGWLISQFGKLRIKQGDYVIIPRTTIYKVE
                     WDKGPVRLLIIESASPVETVKRYRNELGQLMEHSPYCERDIRPPSELITDTGNKETLV
                     KIKKSGYLHQYVYATSPLDLVGWDGYLFPYALSIHDFEPITGRIHQPPPVHQTFQGHN
                     YVICSFVPRMFDYHPLAIPVPYNHSNIDSDEVLYYVEGSFMSRKGISRGSFTLHPGGI
                     PHGPHPGTVEKSLGAASTEELAVMLDPFKPLSLTEDSYPYLDPSYPMSWNE"
     gene            complement(5624..7249)
                     /locus_tag="VGA21_08890"
     CDS             complement(5624..7249)
                     /locus_tag="VGA21_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019598081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEX9650664.1"
                     /translation="MSEIKNSRTQIKLPMLLSMAVAIGLLFGAGISGNEGGGENPAES
                     ATKLKEVLTLIDRNYVDDVDINEITENTIRNMLHNLDPHSYYIPAMDLNRVNSELQGN
                     YEGIGIQFDVVRDTIYVVTALGGGPSEKMGIKSGDKIVSVDGDNVGGIGIDYQGVYNR
                     LLGPKGSQVRIEIKRRGERELLPFLITRDKIPTNSVDVYYMITPEIGYIKFRNFSSTT
                     YDEVVEAISKLEKDGMKKLVLDLQDNRGGYMIPAVNLADEFLQGNKLILTQKDKDPKR
                     TEKYFSKRKGLFEKGPLIVLIDEGSASSAEILAGALQDHDRALIVGRRSFGKGLVQVQ
                     YPLSDGSEIRLTISRYYTPSGRSIQKPYGNGNEYENDWINRYNHGEFFSADSVKFADS
                     LKYETAKGRVVYGGGGIMPDHFVPLDSSFHSTYYTHLFGNNVLREFSYLYYENNKASL
                     KEMGFEKYNHEFKVTGEMFDKVIAIGEEGGVVYNESGFLKSKPLMELTIKAFIARSIW
                     GEKEFYQVFNESDEVLQQSLKLFDKAEQLALAN"
     gene            7385..8137
                     /locus_tag="VGA21_08895"
     CDS             7385..8137
                     /locus_tag="VGA21_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017733722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair di-iron protein"
                     /protein_id="HEX9650665.1"
                     /translation="MSTSFNNRRINELVDENYVYASVLFHLGIQFYDYSEKTLEEACE
                     EHGLNLNNVIRSLEASVNRGDETNPVLKSYPIDLIIEYLKHAHHIFVKRRLPYLAKII
                     ETLDEKNGYQSICSDLKFVFPLFVEDFIHHIYEEEDNLFGFILDLQKFNKGKLSTGKL
                     YYQIEKFSIRKYAAEHEAHDDEMNGIRRITGNYSVNQKASLHLTVVYAELQAFERELK
                     IHSMVENNILFPKAINLEKKALKTFRQKINDN"
     gene            8137..8550
                     /gene="ruvX"
                     /locus_tag="VGA21_08900"
     CDS             8137..8550
                     /gene="ruvX"
                     /locus_tag="VGA21_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013926566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEX9650666.1"
                     /translation="MGRILALDYGLKRTGIAVTDPSRIISTPLLTIHTSGLFEFLKSY
                     FMKEQVEIIVVGMPRKLDNTPTDMTKPVEKLVDKLKKTFPDKKVTLQDERFTSSIARA
                     AILESGMKKKGRRKKENVDKISATLILQSYMESQK"
     gene            8553..9104
                     /gene="def"
                     /locus_tag="VGA21_08905"
     CDS             8553..9104
                     /gene="def"
                     /locus_tag="VGA21_08905"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011584096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEX9650667.1"
                     /translation="MIYPIIVYGDPILKKRAEEIKKNELDVLQLAEDMFETMYNASGV
                     GLAAPQIGISKRIFVIDGSAVEEPQLEGFKKIFINPEIKDEDGLPWPYEEGCLSIPNI
                     REDILRNPKIRVTYLDENWEEHTEEYEGLKARIIQHEYDHLEGILFTDYLSGFKKRLL
                     KGKLQNIKKGNVETSYPIKVIVK"
     gene            9101..10261
                     /locus_tag="VGA21_08910"
     CDS             9101..10261
                     /locus_tag="VGA21_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009280825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine aminotransferase"
                     /protein_id="HEX9650668.1"
                     /translation="MSIGKAKLLQSKLPDVGTTIFAVMTKMANDHGAINLSQGFPDFE
                     VSKELIDGIYKSMLEGKNQYAPMPGLPQLKTGIQSMIRNSYGHNPDPDSEITVTAGAT
                     EAIFSSLMALVNTGDEVIMFDPAYDCYDPAVKLAGGISVHIPLTQPDFHIDWDRVRKS
                     ITPNTRLIMINSPQNPSGAVLYESDLDELEKVIDNNDILVLSDEVYEHIIFDGIMHQS
                     ILRRESLRENGLAIFSFGKTFHATGWKVGYVISPEFITGEVRKVHQFLTYSVNTPMQW
                     GLAAYAENPKNYLNLPAFYQEKRDKFLNLIKDSGFTPVPCHGTYFQLLSYSGIDSIPE
                     MQMAEELTKKHGLASIPVSVFYADRQENNLLRFCFAKNDDTLQQAAEILCKI"
     gene            10249..11046
                     /locus_tag="VGA21_08915"
     CDS             10249..11046
                     /locus_tag="VGA21_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013453719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX9650669.1"
                     /translation="MQDLKVTFIQIDNHWHDVAANLALFGEKISQIEPETDIILLPEM
                     FSTGFTMEVQKMAEKMDGKTVRWMTEQASKNRVLILGSIIIEENGKYFNRLVWMQPNG
                     ELDFYDKRHLFRMADEEKYFSMGSKRIIKEWKGWKIFPLVCYDLRFPVWCRNTFDKKT
                     GTGDYDVLIFIANWPQPRINAWDALLRARAIENLSYCIGLNRIGEDGAHNLYNGHSAA
                     YSPKGDQLFFAEADERIETVSLNYQELAEFRQKFPAYRDADNFGLSL"
     gene            complement(11209..11451)
                     /locus_tag="VGA21_08920"
     CDS             complement(11209..11451)
                     /locus_tag="VGA21_08920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650670.1"
                     /translation="MQVKIYPNPVYDRLHSELQIIANKDFTLEIFSLMGVGLIEGVEI
                     NENLVREIDVSNFSSVSVKITMKLISNVCHTSVFIG"
     gene            11746..13035
                     /gene="murF"
                     /locus_tag="VGA21_08925"
     CDS             11746..13035
                     /gene="murF"
                     /locus_tag="VGA21_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018472796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEX9650671.1"
                     /translation="MERIDIKVLYEHFKSSSGICTDTRKIIPGSIFFALKGPTYNANE
                     FALNAIKGGAAYAVVDDENLPDSPSFILVDDVLSTLQELAKYHRSQQDIPVIGITGSN
                     GKTTTKELISRVLAKKYKTFATKGNLNNHIGVPLSILSITPEVEIAIIEMGANHIGEI
                     ESLCEISKPTHGIITNIGKAHIEGFGSFEGVMKAKSELYQYLLNHNGEVFINSKDLIL
                     ANMAKRFPKPNFFPDQGDYFEAKLVVANPFVSYKSENGETVKTHLIGSYNFINICAAL
                     CIGKFFEVNPKLANEAVKDFVPGDNRSQVLNKNDNTIFLDAYNANPDSMNVALVNFES
                     IPAENKVVILGDMNELGSISKKEHQNLVVRTAEKFQKIFLCGPLMQDARHYNPKALWF
                     PNPEELIAHLQEAPIRNSLILIKASRGIGLERVVEGL"
     gene            complement(13214..13951)
                     /locus_tag="VGA21_08930"
     CDS             complement(13214..13951)
                     /locus_tag="VGA21_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018343642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEX9650672.1"
                     /translation="MFRSANPALTQEVFTKESLLTTEETMTIQGCVNKTLLLLFMVMF
                     PASFVWVKFFQGVNIMPFMIGGAIGGFIFAIITIFKRSWAPYTAPLYATAQGVFLGGL
                     SGFMESMYPGIVLQAVALTFGTLLSLLLAYKSGFIKATPGFKRGVFAATGAIAFIYFI
                     TFILGFFGIQIPFIHSNGLLGIGFSLVVVVIAALNLVMDFDFIEKGSESGAPKYMEWY
                     AAFGLMVTLIWLYIEFLRLLSKLRSRN"
     gene            14157..14816
                     /locus_tag="VGA21_08935"
     CDS             14157..14816
                     /locus_tag="VGA21_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017732814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 domain-containing protein"
                     /protein_id="HEX9650673.1"
                     /translation="MNSAFILSFLSDLRRNNNRNWFEKNKYRYKEALGYFTEVVHEII
                     KELGRTEPEISLLEPKDCIFRIYRDIRFSKDKTPYKLHFGAYFSAGGKSGAQAGYYLH
                     FEPEDESFLGGGLYHPAAEKLAKVRQEIDYNASELASIVKGKNFKRIFGEIQGESLVR
                     PPKGYSEDHPQIELLKLKDFFVIHKLSDKSIKEKNFVRNAVSKYKTMKPFNDFLNVAI
                     S"
     gene            complement(14813..17293)
                     /locus_tag="VGA21_08940"
     CDS             complement(14813..17293)
                     /locus_tag="VGA21_08940"
                     /inference="COORDINATES: protein motif:HMM:NF024893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HEX9650674.1"
                     /translation="MKYIKKGFFYLFFTMLVLVIAGTVTVYLYQDKIVGQLIREGNKH
                     LLTPIHAEKIEATIWKNFPSVSVALHNVVIEENTAQHGILATAGLIVVSFSILDLIQG
                     DYVINELQAQNGKVRLAIDKNGNPNYQILKRAEKSSGGMVVDLKKVFLTRINLNFINE
                     AKDSEYDLDLHSVNASLQILNNYVITRLHGDITSNYLRFENDKFIRAQDWKVNSDLTY
                     NLETKFLDIAQCDLSNARGNYVITGNYDFNTSFTELDFEAKDSDIQTILNFLPLENQN
                     RLSRYQSEGDIYLKGKLTGIISSTKSPEVVINFGTEKAAIFHPEYQSRLTDVQLTGEF
                     RTKNLNDPTQATLKLTNILCWLEGKTFTGDLTITNFKDPWIETVFTGAMDMSSLFSFY
                     PVERIDQASGAIQFNIDFTGKLSNLKNKSTVDRVEMSGDIAMLDVNVKWKGNKLPFQN
                     LNGSLLFNNSDLALNEVTGNLGNSDFMLNGFFKNIITYLLLDQKTFGIEADLKSDLID
                     LDQLLASTENPREEYVFRIPPELFLSFDCDVKRVKFRRLDARWVTGKLKVKDQLAVGK
                     DIRAMTMGGQLDISGLINAREENHITVNAEGKLTRINIDSVFYVFENFNQDWLIDKNL
                     KGQVYADVLTEMAFRKDLTFISPEFIADIGISIRNGQLLNFEPMQELSRFVDGEDLSD
                     LRFSDLKNDIHIQNRTIFLPEMEVSSNITSLTLNGTHTFDQVIDYRVIVPFRIKARMD
                     KDEYYGAVEDDGTGKSKLFLRITGTTSDYKVTYDTEAVKKKIIADLKKEVNDLKAAFK
                     NKGAKRRAVLGLNEDEYFDWDHENNNNR"
     gene            complement(17449..18171)
                     /locus_tag="VGA21_08945"
     CDS             complement(17449..18171)
                     /locus_tag="VGA21_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020532113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX9650675.1"
                     /translation="MKLRCIVIDDEYPARVLLKDYIDNLPQLDLINSFKNPLEAMETL
                     SSENTDLIFLDIQMPGLTGIEFLKSMRRIKPMVIFTTAYPDYALEGYTLDVVDYLLKP
                     FTFERFVAAVQKASDLHNLRKGKPGNVTSEEIKKSGPNFMQIKSGPKKYLVNIEDILY
                     VEGLREYVTYVLESQKIISLESLRSLEQSLPADLFLRVHKSFIINKKKVQSIEGNQIE
                     IKGRKIPIGKSYKEIVMKKIFQ"
     gene            complement(18172..19218)
                     /locus_tag="VGA21_08950"
     CDS             complement(18172..19218)
                     /locus_tag="VGA21_08950"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX9650676.1"
                     /translation="MSKQLKVPALIIHLLLWAGYGLVVFYLMSFPFESQYAWALSARV
                     VFVHALLFYLNTEIILPKLLERGKYAVYVFGLLTLIIGVYYFFNFTSEVGIFREALDS
                     IRPRRQFQGPRPIFSRWMISNMLSSTAILFISTTYWLINRERRRKQQELSLINENLQS
                     EMKFLKSQINPHFLFNALNNIYSLMRSNSQNAQQMILKLSEMLRYVIYDSNEKKVPLV
                     KEIDYIINFIGFQKLKYEEEVNITADFQNVDGSLYIEPMLFIPFIENSFKHSRVEDDK
                     NSWIDLKIQTSGNKVLFILKNSFPPTEFSKDATGGIGIQNVKKRLELLYPGKYVLNIE
                     ETEKEFSVSLEIET"
     gene            19427..19810
                     /locus_tag="VGA21_08955"
     CDS             19427..19810
                     /locus_tag="VGA21_08955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650677.1"
                     /translation="MKRLSYSLIIIFSLFAFDKLIAQTRQGGGQGFNPAEMIEAEKKA
                     VLDRITTLTEDQKEVIEIIYTDYTESFGKAREKSSGDFQVMRTKIVELRAEKDKSLKE
                     VLTEDQYKEYIALMEEIRQRRIRGN"
     gene            19815..22643
                     /locus_tag="VGA21_08960"
     CDS             19815..22643
                     /locus_tag="VGA21_08960"
                     /inference="COORDINATES: protein motif:HMM:NF026255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX9650678.1"
                     /translation="MKKIIFSLVLVLSSYAAWAQTFSLTGTLKDSVDNSPLIGANIIL
                     TSVRETDKKFYAVADINGNFQVTGLEKTFYKMEVSFVGYGTLKKFVRITDPVTTLGNV
                     LMNEDAELLQEVTIEGETVASVQKGDTTQFNANAFKTNPDANTEDLIRKMPGITVENG
                     TVQAQGENVGKVLVDGREFFGNDATVALRSLPADVVEKIEVFDRLSDQSQFTGFDDGN
                     TTKTINIVTKSTLKTSQFGKIYAGYGTDNRYLAGGNLNIFNGDQRISFIGMSNNISEQ
                     NFSTQDLLGVVGTSNQRRGFGGGAGGGFTGGRPGFTGGNFRFGGTSNFLVGQQNGITS
                     TNSFGINFMDSWGEKVNITASYFFNMTNNDNLQLVSQEYFLDDNNQFYDESTISRADN
                     MNHRLNLRLEYTLNERNSLIWTPTVNLQDYESSSQVAGVNYLDETNLLSETENFNSAN
                     NYGYNIASNLLLRHSFAKRGRTLSWNIRTDYNEKDGDAFLTSSNNFYNGPVYSSDSLL
                     QQTMTGSDGYTFASNLVYTEPLGESGQLQLNYNTSFTRSNSTRYTYDFNYSDQLFSVL
                     DTALSNTFMNDYLTQRAGGSYRLRKGKVFFTLGADFQNANLVGDQTFPVETSGEKTFN
                     NILPNAMFMFTVSRNTNLRIFYRTRTNAPTISQLQNVVDNSNPLQLTTGNPDLKQQID
                     HFVIARYSLSRVESNSTFFALAFLRFADNYLGNSTIIADQNMVLPDGTVLNRGSQFSM
                     PVNLSGYWSGRGFLTYGFPLRFVKSNLNLSSSYNYSRTPGLINNALNNSDSHTFSEGI
                     TLSSNISEKIDFTLSYSANYNIVENSIRPELDNNYFYHTAGFGSAITFWKGLVLRNDL
                     NNYLYRGLSDEFNQDFFLWNISLAKRIFKEQNGEIRLGVFDVLKQNRSITRNVTETYV
                     EDVNTEVLKQYFMLTFTYNLKALGQKKG"
     gene            complement(22603..23184)
                     /locus_tag="VGA21_08965"
     CDS             complement(22603..23184)
                     /locus_tag="VGA21_08965"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX9650679.1"
                     /translation="MVSISDKSLIKFELVQDADLHSLHEVSTLAYFQAYFGSYTYNDL
                     KPYLEDFFSEETLKKEIAKQDQDYFLVKKSDRAIGFAQVRRFILIEDSIRKRSDNSAE
                     LFKLYLLQKYTGKGIGTKFMQMMSEHYQSRGFQNFYSTVWSLSPRALRFYKRQGFDVI
                     GEIDYDYAGKNNIDYVLKKNLILSSDLMPSGYK"
     gene            23265..25325
                     /locus_tag="VGA21_08970"
     CDS             23265..25325
                     /locus_tag="VGA21_08970"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX9650680.1"
                     /translation="MKTLGYYFTLCLLIVSAQYVNAQYNLEQTLGAPYVQSIFPSEDG
                     KSLAWIINEKGIRNIWFTEVNATAPRKVTAYDRDDGQDLGNITITSGFLIFTKGGGSN
                     RYGDIANPASLPQVPKREILRLNLSNDSISKVDEAAVAVIAPDGKRMLVTKQSRVFLI
                     EDISAPEIKSEVLFEVRRSAGSLSWSPDGNKIAFVSFRSDHSFVGVYSIEENKITWLA
                     PSLFFDSDPAWSPDGSKIAFIRTPGAGRGELRNIMSGVQFEIVVANVNSGTGKTIWKS
                     PGKDGGYSQYYPNAPLRWTKENKILFYSEHEGWNHIYSITPEGSGLKDLSPGECEVEN
                     SALSANHRYLYFDSNCTDINRRHIWKVTVATGQSQMVTDKSGIQSNVVTLEGEAIAYR
                     EGRHNIPTTALIQNGSTTTNLVSLPDNFPASQLVMPEAVQFKSADGLTIHGQLFLPSK
                     LDKTAKLPAVIFMHGGPIRQMLLGYHYSGYYTNAYILNQYMASIGYSVLSVNYRNGIG
                     YGRDFRLDENQGPRGASEYKDIIAAGKFLQSRPEINPEKIGLWGGSYGGYLTAMGLAK
                     NSDLFKAGVDLHGVHDWAWRGEEFSPGGGWGITPELMELAYQSSPISEMQFWSSPVLI
                     IHGDDDRNVMFGQSIDLQKKLDELGVHNEILVFPDEVHGFLRYESWLRANKATVDFFN
                     RFLK"
     gene            complement(25326..25634)
                     /locus_tag="VGA21_08975"
     CDS             complement(25326..25634)
                     /locus_tag="VGA21_08975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650681.1"
                     /translation="MKILKTLLGVFILIIFLISCKENCLLKSKGTLKLTNTSSSTVMR
                     SLVDGVNHGTLDPGESIEIDLGTGVHAFIFEGISGGAGCSPGTVTIVACETSAFTCSG
                     "
     gene            25773..26810
                     /locus_tag="VGA21_08980"
     CDS             25773..26810
                     /locus_tag="VGA21_08980"
                     /inference="COORDINATES: protein motif:HMM:NF017773.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9650682.1"
                     /translation="MKWLRLLWIFPFLLILSFGRPDKHQLYNRWDNIHFVKEIDFDPH
                     PDTVFVICTNRLYQPGLQQPLADRADPENKLQYLVATYSNNEWRLLRKQNLEEAVAYL
                     PRKDFLLYIEGMGKTFPMDLQRSAGMTAQYNLNVIQFEYPSIHPRKNPVANFYFAYNQ
                     AKHATEEYSEFIREFEVLKNNHPEKFAEVNVSLFHHSMGNRMLKHSLEENLLIVKDKS
                     LFQNVILNAACVPQSKHTEWIEKIDFSERVFINYNKKDFQLNGAMFITFDKMLGARFK
                     EPLAGNATYIDFQTLVAGRHSNFLNIDGRDNINPECRRYYDLVLHGNNPNLADSSLFI
                     TSTEIVGFGLK"
     gene            complement(26940..27572)
                     /locus_tag="VGA21_08985"
     CDS             complement(26940..27572)
                     /locus_tag="VGA21_08985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650683.1"
                     /translation="MIKNGILFLIASLIFASCIHIVEDNTTIVKGYIREIRSGKPIEG
                     AGAYLSFKPFLKPEETITHFDNISDSNGYYEIKFEADRGNGYKIAIGRQGYYCNQCNE
                     PVKSGEENTLNVYLVPTAEFTLNLVNDIPYNDSDSIYFCMDGEHLLSNDKECLYCLGP
                     CNENVFRNYTFESNATINLTWWVTENGITTPYSHDIELVSVDTVEYTIRY"
     gene            complement(27569..27925)
                     /locus_tag="VGA21_08990"
     CDS             complement(27569..27925)
                     /locus_tag="VGA21_08990"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEX9650684.1"
                     /translation="MFQQTQVSLAFEVLSDNDEACGQSQQKLVQEFADHLELSPNPAA
                     DKTMIEIRDIPPGIISLKLLALEGNLIKEILGGYTIESGTYKYELDVTQLKTGIYLIS
                     LESREGIENKRLIIVR"
     gene            complement(27941..28156)
                     /locus_tag="VGA21_08995"
     CDS             complement(27941..28156)
                     /locus_tag="VGA21_08995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650685.1"
                     /translation="MNGNKRSIEGPETYCPGLAQDMFTLRNSGLVKRISTSWWLDNDT
                     ERTEASYYQLPISPDQGGHVIHCEVSL"
     gene            complement(28161..>28808)
                     /locus_tag="VGA21_09000"
     CDS             complement(28161..>28808)
                     /locus_tag="VGA21_09000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650686.1"
                     /translation="LNDSEMIKNGILFLIAGLIFASCIHIVEDNTTIVKGYIREIRSG
                     KPIEGAGCYLSFKPFLKPEETITHFDNISDSNGYYEIKFEADRGKGYKVAIGRQGYYC
                     NQCNEPVKSGEENTLNVYLVPTAEFTLNLINEPPSTQADSIYFCMDGEFLSSTQKDCW
                     YCLGPCNENVFNKFTFESNAIIVLTWWVTENSITTPYSHYVELVGLDTAEYTIRY"
BASE COUNT         8841 a         5567 c         5834 g         8566 t
ORIGIN      
        1 gctgttaccg aaaggtgaga gttgtaaatt aggaaccgcc ctataccgat cggtaccagg
       61 gggtgtgaaa ggacagatag ggaaataatc cctatcttcc tactggattt ctaacctaat
      121 cttaaaaaat aatggaagta gatttaaaaa accttagtgc ttatactgat gaagccataa
      181 aattggctct ggcttatgga ccaaaattag tccttgcgat tcttacactt atagttggac
      241 tatgggtgat taaatggttc agcaagggga tgatcaaagt gatggactcc agacgtctgg
      301 atccctcatt gaaaccattt tttagttccc tcatttcaat aaccctgaaa atactcctcg
      361 cgatatctgt aatgggaatg attggcattg aaatgacttc ttttatcgct ttactgggtg
      421 cggccggttt ggcttttggt atggcactat cgggcacgtt acaaaatttc gccggtggag
      481 tggttctact agtttttaag ccctttaaag ttggtgattt tgtggaagcc gccggttaca
      541 gcggaacagt aagtgagatc aggattttca ataccatatt aaaaacccct gataataaaa
      601 cgattatcat tcctaataat gcaatcgcag gcagttcaat ggtaaactac acaacggagg
      661 ctacccgcag ggtcgatttt gttttcggaa tcagttatca agatgacatt gacttaacaa
      721 aatcaatttt gaaccgattg attgaacgtg atgaaagaat tcataaagat cctgctccat
      781 ttattggcct gacagaaatg gctgacagtt cggtcaattt agttgttcgt gtttgggctg
      841 atacacccaa ttattggccc atttattttg atatgatgga ggacgtgaag aaaacttttg
      901 atgccgaagg tatcagtatt ccttttccac agcgggatgt acacctgtat aatcaaaagg
      961 cttaacttgt gataaggaga atacttttgt tcatgctggt gccggtgtca ttccttgcaa
     1021 aatctcagga agataccacg tattggagag caggtcttag ctccacaatc gctttctcgc
     1081 aattgtcgct caccaattgg gccagcggtg gtcagaacag tattgccttg aactctggtt
     1141 taactttgtt cgccgattac gctaaagaaa aaataagctg gaaaaataaa attgacctgg
     1201 cttacggatt tatacaacaa aaagatgatg gatatagaaa atctgatgat agaattattg
     1261 taatcactga gtttggctat ttgattagcc gtaagccaaa tcgaatgaag tggacaacaa
     1321 tgattgattt taaaacacaa tttgataagg gatataatta ccctaatgat tcaatgaaaa
     1381 tatcggattt tatggccccg ggttatttaa caattgtaac cggtttggag tattccccaa
     1441 aacctcattt aacagttctt tactcacctg tttcggggaa attcaccttt gtaaatgatc
     1501 aaagactgtc taacgcgggc gggttcggtg ttgaaggtgc tgtttacgat attgacggga
     1561 ttttgataca ggatgggaaa aacctaaact ctgaatttgg ttcctatatg agggtccagt
     1621 ttgagaagga agtaatgaca aacgtcacag tattttccaa agctgaattt ttcactgact
     1681 acctgatgga ttttggaatt attgatgtga actgggagtc caaacttgac atgaaaatta
     1741 acaactggct ttccgcaaat cttatacttc atctaattta tgaccgcgat attcgctttg
     1801 aagttactga cgcgaatgga ataatcaccg ggacagagga cagaattcaa ttcaaagaat
     1861 acctgggtgt aggatttaag tacaacgtgt caaaccggta agcctcataa taattcccgg
     1921 aattgataaa ttttaagagc taaaaatggc tgccagccgc tactttgtta atttagagca
     1981 atgaatgtta tcctttttga ctacaacccc tggcgagatt cgctgcaacc cttaacctat
     2041 acccggccgg tttcggaact ccgggttggt attcttacta taaaggaaaa atgggaaagt
     2101 gaattatccg tccgtatttc ttacctaacc cggaattatc tttccgagaa atttcccctt
     2161 tctatttcag acgaaaacct cttgataaac ggatcgtggt gtcccatggc aggtgttaaa
     2221 gatcttctcc aattaaatac cggtgaaagc ctcgtatggc aggggattcc acttgcgctg
     2281 aaagtttcta aagatgggtt ttctaaaatt cataatcagg atttcaaaac aaaaaaagaa
     2341 attcaatgtg aaaaagttcc ggttctgatt tctaaacttt ggcatctttt caaaaataat
     2401 ggcagtgaaa tacggattga ttttgagaga ttaaccaaag gcaggaaaag tgaaccatta
     2461 accgatcccg gatgtacaat ctatgggaaa gagaatattt ttcttgaatc cggagcaatg
     2521 gtcagagcgg caatcctgaa tgccgaggat ggacctatat atttggggaa aaattccaga
     2581 gttcaggagg gaagcattat aaaaggtccg tttgccatgg gcgaaggctc ccatataaat
     2641 atgggagcga aggtaaaagg ggattctacc cttggtcctt tctgtaaagt tggtggggag
     2701 atcagtaatt ccatcctgct gggttatagt aataaggcgc atgatggctt cctgggaaat
     2761 tcggtaatcg gagaatggtg caacatcggc gcggatacca acaattcaaa cctcaagaat
     2821 aattatgatg aagtaaagct atgggatttt agccgacaaa gattcgagct aacaggcgag
     2881 acatttgttg gattattcat gggcgatcat tgcaaatgcg caataaatac aatgttcaat
     2941 acagcaactg tgactggagt agcgtccaat atttttggag ctggatttcc aagaactttc
     3001 attccctctt tcagctgggg aggtccggcg ggtttcatca catttaaatt ggaaaaagtt
     3061 ttcgaaatgg ccggggcaat gatgaatcgt cgtggcgtgc aattaacgga ggtcgataag
     3121 aaaatactga caaaggtttt cgaactatcc gtggaataca ggacctggga acggtgattt
     3181 attatattat aaaggtgaga tttgaagatc acaataattt ctttttccta agaattttca
     3241 agtattcacc ttttgaaagt gggcatttcg caagatcaac aaattgcatt ttgtcaagct
     3301 ggcattgaac ggacatttgc ctaattaagt aatcatcaag gtctttttta ctaccgtgac
     3361 ttatttttgt gtgtaatatt aacttatcat tatagtagta ttcgagtctt ttgtgatcac
     3421 caggagaatc tataaatccc tttttcttta aatttctgtg agtgtctttg gaatcaagta
     3481 ctgacattac tcaacagact ttataaggtg attaaggaaa accttaattc tgtttaattt
     3541 ttttgaaaga gaattattat ctaattcatt atatctcttg tatatataat caaattcttc
     3601 agcaaaattt tgttcagcct cttcttcaga ttgaccggtt ccgataatgt caagcatttc
     3661 attatgaata acataataat cgttttcctt ttcaagagat gaccgcaggg gaaaatttaa
     3721 gttataaact aattgaccat aagctataat ctgaggagaa taagaaagcg tagtatagga
     3781 tttcttgtat atttcctgaa ttttactttt tgtacctttc ttagtcgtga tcagcttaac
     3841 ccttgccact gcatattcct ccaatctatc gacagctaaa tcttttattt ttgtggttaa
     3901 ttttttttta acttcaggtt ttaacctttt gattctataa atcggtttta gaattttttt
     3961 ctgatcaaca cctacaatag atgtcggagc atcatcaaaa ctgttggtaa aatcataaag
     4021 aacatcaact atctcatttc tcttataaga atccggataa atttcattaa tcttaaaata
     4081 gtttgattta tttgaaagtt tcatgaggtt ttcaaatctc ttatttacca ctcgtctctg
     4141 taactcgaca tttccaatcg aagtttgaag ccttggagaa aagacgaatt ccggaatggc
     4201 acttccatgc ctgaacccag acaactccac tttgaaattc tccaaatgaa taggctcttt
     4261 tgaatcaata tcacacttag ctattgtaat gagaaggttc tgaaggtttt ctgcaatttt
     4321 aatcaaggta tcaacgggta gagtatgaaa cctacccact tcaccttcaa ttagaaagct
     4381 aacaccttta tttaagttaa ttttcatttt ttttggatcc caatattaga tgtttttgtc
     4441 gactaaaaaa atgaagctga tttattcatt ccagctcatc gggtaactgg gatccaggta
     4501 cgggtaagag tcttctgtca agcttaaggg tttaaaggga tccagcatca cagcaagttc
     4561 ttcggtggaa gcagctccca aacttttttc cacagtaccc ggatgggggc catgcggtat
     4621 tccgccggga tgcaaagtga acgaaccccg gctaattcct tttctactca taaaacttcc
     4681 ttccacataa tagaggactt cgtcggaatc aatgttggag tgattatacg gtacaggaat
     4741 tgcaagcggg tgataatcaa acatccgggg cacaaaacta caaatgacgt aattatgtcc
     4801 ctggaaagtt tgatgcacag gtggtggctg atgaattcgt ccggtgatgg gttcaaagtc
     4861 atggatgctt agcgcgtacg gaaataaata cccatcccag ccaaccaggt ccagcggact
     4921 ggtagcataa acatattggt gcagataacc ggacttcttt atttttacca gggtttcttt
     4981 attgccggtg tcggtaatta attctgatgg gggacggatg tctctttcac aatagggtga
     5041 gtgctccatc aattgtccca attcatttct atatcttttt acggtttcta caggcgaagc
     5101 cgactcaatg atcaataacc ggactggacc tttatcccat tcaactttat aaatcgtggt
     5161 tctggggatt attacgtagt caccttgttt tattctcaac ttgccgaact gacttatcag
     5221 ccatccgttt ccatcgtgaa tgaaaataac ttcatcccct tcggcatttt tataataata
     5281 atccatggat ttttcttttg gaatgcacaa tgccagggag acatcgttat taaccagcat
     5341 caccttacgg gcatcaaggt aatcgttacc ggttattcct gcttcggagg tgttcaggtg
     5401 agtcggtttc atgacggtct caatccgttt aggaccgtaa ggcaggggat cctgaatctc
     5461 cttaattctg gtgggtgcct caatgtggta aagattggaa tagatacctg aaaacccttt
     5521 ggagctgact aactcttcct tatacagact gccatcgggc tgacggaatt gcgtgtgccg
     5581 cttaggtggt atttctccca atttgtgata gtatgtcatg ttattaattt gctaacgcaa
     5641 gttgttctgc cttgtcaaat aatttcaatg attgttgaag aacttcatct gattcattaa
     5701 atacctggta gaattctttc tcgccccata tgctgcgggc tataaatgct ttgattgtta
     5761 attccattaa tggttttgat ttgaggaagc ctgattcatt ataaacaaca ccaccttcct
     5821 caccaattgc aataacctta tcaaacattt ctccggttac cttgaattca tgattatatt
     5881 tttcaaatcc catttcttta agagaagctt tgttattctc ataatacaaa taactaaact
     5941 ccctaagcac gttattgccg aagagatggg tataatatgt gctgtgaaaa gaggagtcca
     6001 ggggaacaaa atgatccggc ataattccgc caccgccata aacaactctg ccttttgcgg
     6061 tttcatattt taatgaatcc gcaaatttga cactatcggc gctaaaaaat tcaccatggt
     6121 tatatctgtt gatccagtca ttctcgtact cattgccatt tccatagggc ttctgaatag
     6181 agcggccgct gggggtataa tacctggaaa ttgttaaacg tatttcagaa ccatcactca
     6241 gcggatattg aacctgcacc aatccctttc cgaatgaacg tcttcctaca atcagggcgc
     6301 ggtcatgatc ctgcaatgcc cctgcaagaa tttctgctga agaagcagat ccttcatcaa
     6361 taaggacaat cagagggcct ttctcaaaca agccttttct ttttgagaaa tacttttccg
     6421 tcctttttgg atctttatct ttctgtgtca gaataagttt attgccctgg agaaattcat
     6481 cagcaagatt tactgccgga atcatatacc cgccgcgatt gtcttgcaaa tccagaacca
     6541 attttttcat gccatccttt tcaagtttcg aaatcgcttc aactacctcg tcataagtag
     6601 tgctgctgaa attcctgaat tttatatacc cgatttccgg cgttatcata taataaacgt
     6661 ctacggagtt ggtaggtatt ttatcacggg taatcaaaaa tggaagcaat tccctttctc
     6721 cccgccgttt tatttcaatt cttacctggg agcctttggg gcccagaagc cggttgtaaa
     6781 caccctggta atcaatccca attcccccca cgttatctcc gtcaacactc acaattttgt
     6841 cccctgattt aatccccatt ttttctgaag gcccaccccc aagagcagtc accacgtaaa
     6901 tggtatccct aacaacatca aattgaatcc caattccctc gtaatttccc tgcaattcag
     6961 aattcacccg gttaagatcc atcgccggaa tatagtaaga gtgaggatcc aggttatgca
     7021 gcatgttacg aatggtattc tccgtaattt cattgatatc cacatcatct acataattcc
     7081 ggtcaataag cgtcagcact tctttaagct tcgtcgcgct ttcagcagga ttttctccac
     7141 caccctcatt tccggagatg ccggctccaa acaacaatcc aatggcaacc gccatggaaa
     7201 gaagcatcgg cagtttgatt tgcgttctcg aattctttat ttcactcact ttattcctcc
     7261 ttttactttt aactctggga ttggtaatat aacatgacaa taaatttaca ttatttttga
     7321 cagtttaaga tggataaaat aattgatata attttttatt ttatctttga ttaacgagcc
     7381 aataatgtca accagcttta acaacaggcg aataaatgaa ctagtagatg agaattacgt
     7441 ctacgcgtct gtcctgttcc acctggggat tcaattttat gactattcag agaaaactct
     7501 tgaagaggct tgtgaggaac atggcttaaa cctgaataat gtaatcagga gcctggaagc
     7561 ttcagtaaat cgaggtgatg aaaccaatcc ggttttaaaa tcatacccca tcgaccttat
     7621 tattgagtat ttgaagcatg cccatcatat ttttgtaaaa cggcgattgc cttacctggc
     7681 caaaattatc gaaaccctgg atgagaagaa tgggtaccaa agtatttgta gtgatttgaa
     7741 gtttgttttt ccgctatttg tggaggattt tattcatcat atttatgaag aggaggataa
     7801 tttgtttggc tttatccttg atttgcagaa attcaataag ggtaagctct caactggaaa
     7861 actgtactat cagatagaaa aattttcaat ccggaaatat gcagctgaac atgaagctca
     7921 tgatgatgag atgaatggca ttcgtagaat tacgggtaat tattcagtga atcaaaaagc
     7981 cagtcttcac ctgactgttg tttatgccga actccaggca tttgagcgcg agttgaaaat
     8041 tcattccatg gtggaaaata atattttatt tcccaaggca attaatttgg aaaaaaaagc
     8101 gctaaaaacc ttccgccaaa aaattaatga taactaatgg gtcgaatttt ggccctggat
     8161 tatggtttga aacgaaccgg cattgctgtc accgatcctt ccagaattat ttccacaccc
     8221 ctcctaacta ttcacacttc aggactgttc gaatttctta agagctattt tatgaaggag
     8281 caggttgaga taattgttgt tggtatgccc agaaaattgg ataatacacc cacagatatg
     8341 acaaagccgg tggaaaagtt agtagataaa ctgaagaaga catttcctga taaaaaagta
     8401 actttgcagg atgaaagatt tacttcaagt atagccaggg cggcaatact ggaaagtggt
     8461 atgaaaaaaa aaggtaggcg aaaaaaagaa aatgtggata aaataagcgc gacattaatt
     8521 ttgcagtcat acatggaaag tcaaaagtaa ttatgattta cccaataata gtttatggag
     8581 atcccatcct caaaaaacgt gcggaggaga tcaaaaagaa tgaactggat gtgctgcagc
     8641 tggctgagga tatgtttgaa accatgtata acgccagcgg ggtcgggttg gccgcgcccc
     8701 agattggcat ttcaaagcgc atttttgtaa ttgatggaag tgcagttgag gagccacagt
     8761 tagaaggatt caaaaagatt tttattaatc ccgagattaa agatgaagat ggcttgccct
     8821 ggccctatga agaaggatgt ttaagtattc caaatatcag ggaagatatt ctccgtaatc
     8881 ccaaaataag ggttacttac ctggatgaaa attgggaaga acatacagag gaatacgagg
     8941 gtctgaaggc gagaatcatc cagcatgaat atgatcacct ggaagggatt ttattcaccg
     9001 attatttgtc cggtttcaag aagagacttc tcaaaggaaa acttcaaaat ataaagaagg
     9061 gtaatgttga aaccagttat ccgattaaag taatagtaaa atgagcattg gaaaagccaa
     9121 attgttgcag tcaaaactgc cggatgtggg aaccacaatt tttgcagtga tgacgaaaat
     9181 ggctaatgat cacggcgcta tcaacctgag ccagggcttt ccggatttcg aagtatcaaa
     9241 agaactgata gacggcatct ataaatccat gctggaaggt aagaatcaat atgctcctat
     9301 gccaggactt ccgcagttga agaccggcat tcaaagcatg ataaggaatt cctatggtca
     9361 taatcctgat cctgattcgg aaattacggt aactgccggg gcaaccgaag caatattttc
     9421 atccctgatg gcactggtaa ataccggaga tgaggtcatc atgtttgatc ctgcctatga
     9481 ttgttatgat cctgctgtga aactagcagg tggcatatcc gttcacattc cgctaacgca
     9541 accggatttt catatcgatt gggaccgcgt taggaaaagc atcaccccaa acaccaggtt
     9601 gataatgatt aattccccgc aaaatccttc tggcgctgtc ctttatgaat ctgacctgga
     9661 tgaactcgaa aaagtaatcg acaataatga tatcctggtg ttatctgatg aggtgtatga
     9721 gcatatcata tttgatggca ttatgcatca gtcaatcctg agacgcgaat ctttgcgcga
     9781 gaatggattg gcaattttca gctttggaaa aacttttcac gctacgggct ggaaggttgg
     9841 ctatgtaatc tctcctgaat tcatcaccgg tgaagtcagg aaggttcatc agttcctgac
     9901 atatagtgta aatactccta tgcaatgggg attggctgca tacgcggaaa atcccaaaaa
     9961 ttatcttaac cttccagcat tctaccagga gaaaagagat aaattcctga atcttattaa
    10021 ggactcggga ttcacaccgg tgccatgtca tggcacgtac ttccagttac tgtcttattc
    10081 cggtatagat tcaattccgg agatgcagat ggctgaagaa cttaccaaaa aacatgggtt
    10141 agcttcaatt cccgtgtctg tcttttatgc cgacaggcag gaaaataacc tgctccggtt
    10201 ttgctttgca aaaaatgatg acactttaca acaggcagcg gaaatactat gcaagattta
    10261 aaagttactt tcatccagat tgataatcac tggcatgatg tagcagccaa cctcgctttg
    10321 tttggtgaga aaatttctca aatcgaaccg gagactgata taatccttct gcctgaaatg
    10381 tttagtacag gctttaccat ggaggtgcag aaaatggccg aaaaaatgga cgggaaaacg
    10441 gtacggtgga tgacagaaca agcatcaaag aacagagtcc tcattctggg aagcataatc
    10501 attgaagaga atggtaaata tttcaaccgc ctggtttgga tgcagccgaa tggtgaactg
    10561 gatttctatg ataaacgtca cttgttccgc atggctgatg aggaaaaata cttttcaatg
    10621 ggctcaaaga gaattatcaa ggaatggaag ggctggaaaa tattccccct tgtgtgttat
    10681 gatttgcgat ttcctgtatg gtgtagaaat acttttgata agaagaccgg aaccggagat
    10741 tatgatgtac tgatctttat agccaactgg ccgcagcctc gcatcaatgc ctgggacgcc
    10801 ttgctgagag ccagggcaat tgaaaacctg agttattgta tcggactgaa ccgtattggt
    10861 gaagatggag cgcataactt atataacggc cactcggccg cttactcccc gaagggggat
    10921 caactatttt ttgctgaagc tgatgaaaga attgaaactg tgtctttgaa ttatcaggaa
    10981 ctggcagaat tcagacagaa atttcccgct tatagggacg cggataattt tggattgagc
    11041 ttatgattat caattcataa caattactct aaaggtttct tgcattgagt gagtatagaa
    11101 cctgaagaaa taaattccag gtcggaaatt tgggtagtgt ctcagtttgg aatcattata
    11161 cgtcattata cgtgtgccac actttttcga cacggaaaag caacaatttc acccaataaa
    11221 gacggaagtg tggcacacgt tagatattaa tttcattgtg atcttaactg agacactact
    11281 gaaatttgac acatctattt cccgaaccaa attttcatta atttcgactc cttcaattaa
    11341 tcccacgccc atcagactaa aaatttcaag cgtaaagtct ttgttggcaa ttatctgaag
    11401 ttcagaatga agtctgtcat aaacaggatt gggataaatc ttaacttgca tcccgggtag
    11461 ctctccttcc cgattatttt caaagaatat gcaatccgcc cagcggaatg gtaagagtcc
    11521 ggttcaaatc aaagaattga atttcaggat tttctctaaa ataaaattgt ccgaacccga
    11581 gtaaaggaac taaaataaga gaagcagata aatgaaattt cactcaaacc ttttttacaa
    11641 aaatgttacc aaattaccgg caaagataaa cttttaataa gcaatctgac taaacctcct
    11701 tgtaccataa taatattact atctttagat gttgcagccc taacaatgga gcgaatagat
    11761 atcaaagtcc tatacgaaca tttcaaatct tcttcaggca tctgcacgga tacaaggaag
    11821 ataattcccg gctctatttt ttttgctctg aagggcccaa catataatgc aaatgaattt
    11881 gcattgaacg caattaaagg tggagccgcc tatgcggtgg ttgatgatga gaatttacct
    11941 gacagccctt cctttattct ggttgatgat gttttatcaa ccttgcagga acttgccaaa
    12001 tatcatcgtt cgcaacaaga tatacctgta attggtatta caggttctaa tggcaaaacc
    12061 actacaaagg aactgattag cagagtcctt gcaaaaaaat ataaaacctt tgcaacaaaa
    12121 gggaatctta ataatcacat cggtgttccg ttaagcattc tgagtattac tccggaggtg
    12181 gaaattgcta tcattgaaat gggtgcaaat cacattgggg agattgaatc actttgtgaa
    12241 atttcaaagc caactcatgg tataattaca aacattggca aggcgcatat cgagggattt
    12301 ggaagttttg aaggcgtaat gaaagctaaa agtgagttgt atcaatattt gttgaatcat
    12361 aatggagaag ttttcattaa ttccaaagat ctaattctgg ctaacatggc caaaagattt
    12421 ccaaagccta acttctttcc tgatcagggt gattattttg aagcgaagct tgttgtcgcg
    12481 aatccttttg ttagctacaa atcagaaaat ggcgaaacgg taaaaactca cttgataggt
    12541 tcttacaatt tcattaacat ttgcgcggcc ttgtgcattg ggaaattttt tgaggtgaat
    12601 ccaaagctgg caaatgaagc tgttaaagat tttgtgccgg gtgataaccg gtcacaggtt
    12661 ctgaacaaaa acgacaatac cattttcctg gatgcataca atgctaatcc tgacagtatg
    12721 aatgtcgctt tggtgaattt tgaatctata cccgccgaaa acaaagttgt aattttggga
    12781 gatatgaatg agctgggatc aattagcaaa aaagaacatc aaaacctcgt ggtaagaacc
    12841 gcggagaaat ttcaaaagat cttcttatgc ggacctttga tgcaagatgc ccggcactat
    12901 aatccaaagg cattatggtt tcccaatccg gaggaattga tcgcgcactt gcaagaggcg
    12961 ccaattagaa attctctgat cttaatcaaa gccagccgcg gtatcggact ggagcgggtg
    13021 gtggaaggtt tgtaatcccg aatccggatc cagagcattt ttcagaattt ggcctgacag
    13081 tgcaattaaa atttcttatc accgaattcc gttctcataa aattgatgcg ctgagcttca
    13141 tcaatactat tttgctttga cccttttaac gagattttct gtaattgcct tctatcgagt
    13201 ttgggttcag ccgctaattc ctgcttctca acttactcaa caacctgagg aattcaatat
    13261 ataaccagat caaggtgacc atcagaccga atgcggcata ccattccatg tatttgggag
    13321 cgccggattc agatcccttt tcaataaaat caaaatccat tacgagattt aaagcggcaa
    13381 taacaactac taccaaactg aacccgattc caagcaaccc gttggaatgg ataaaaggga
    13441 tctgaatacc aaagaaacct agaataaaag tgatgaagta aataaacgcg attgcgcctg
    13501 tcgcggcaaa tactccccgc ttaaatccgg gggttgcttt gataaaacca gatttgtagg
    13561 ctaataaaag ggaaagcaaa gttccaaaag ttaatgctac tgcctgcaac acaattccag
    13621 gatacattga ttccatgaaa cctgaaagac cgccaagaaa aactccttgc gcggttgcgt
    13681 aaagaggcgc ggtatatggt gcccaggagc gtttaaaaat tgtgataata gcgaaaataa
    13741 atcctccaat tgctccgcca atcatgaagg gcataatgtt aactccctga aaaaatttca
    13801 cccatacaaa agatgcagga aacattacca taaataacag aagcagggtt ttatttacac
    13861 aaccctgaat cgtcatggtt tcttctgttg tcaatagact ttctttggta aaaacttcct
    13921 gagttaaagc tggatttgct gacctgaaca taattgaata aatttttgtt caaaggtaac
    13981 gtatgggcag gactcattaa atatcctgaa ggcaagaaaa aagcccgaat gtggaatccg
    14041 ggcttcaacc taacctatat tgacataaag agtttttgac cttggaggtc gtttaattat
    14101 agatgcaggt tcagtgtgaa aagttgtaca ttggaggcat tttttttaga gaatccatga
    14161 attctgcttt tattttaagc tttttaagcg atttgagaag aaataacaac aggaattggt
    14221 ttgaaaagaa taaatatcga tataaagaag ccctcggtta tttcacggaa gtggttcatg
    14281 agatcatcaa agaattagga agaacagagc ctgaaattag tttgctggaa ccgaaggact
    14341 gcattttcag gatctaccgg gacattcggt tcagcaagga taagactccc tataaattac
    14401 attttggagc ttatttctcg gccggaggta agtcaggcgc acaagcggga tactatttac
    14461 attttgagcc tgaagatgag tctttcctgg gaggtggttt ataccaccct gcagcggaaa
    14521 agcttgcaaa agttcgacag gaaatagatt ataatgcatc cgaacttgcc tcaatagtca
    14581 aagggaaaaa tttcaaaagg atttttggtg aaattcaggg agagagtttg gtgcggccgc
    14641 caaaaggata ttcagaggat cacccgcaaa tagaattact caagcttaaa gacttttttg
    14701 tgattcataa attaagcgat aagtcaataa aagaaaagaa ttttgtaaga aatgcagtta
    14761 gtaaatataa aacgatgaaa ccatttaatg atttcctgaa tgtggcaatc agctaacggt
    14821 tgttattgtt ttcatgatcc caatcaaagt actcatcttc atttaatccc aacacggccc
    14881 gccttttagc tcctttattt ttgaatgcgg ctttcaaatc attaacttct ttctttaggt
    14941 cggcgataat ttttttctta accgcttcag tgtcataagt aactttataa tcactggtag
    15001 taccggttat gcggagaaac agtttacttt ttcctgttcc atcgtcttct acagcaccat
    15061 aatactcatc tttatccatg cgcgccttta tacgaaaagg tacaattacc ctataatcaa
    15121 ttacctggtc aaatgtgtgt gtaccattca aagtaagact tgtaatgttt gaagaaacct
    15181 ccatctcagg tagaaagata gttctgttct ggatatggat atcatttttc aaatctgaaa
    15241 atcttaaatc agacaaatct tctccgtcaa cgaacctgga aagttcttgc atcggctcga
    15301 agtttagtaa ttgcccattc cggattgaaa ttccaatgtc agcaatgaat tcaggagaaa
    15361 taaaagtgag gtctttgcga aaagccattt cagttaagac atccgcatat acctggccct
    15421 tcagattctt atcaataagc cagtcctggt tgaagttttc aaaaacgtaa aaaacgctgt
    15481 caatgttaat tcgggtaagt ttgccttccg cgtttacggt gatatgattt tcttcccggg
    15541 catttattag gccgcttata tccaattggc ctcccatagt catagcccgg atgtcttttc
    15601 caacggcgag ctggtctttc accttcaatt taccggtaac ccatcgggca tcaagtcttc
    15661 gaaatttcac ccgcttaaca tcgcagtcaa aagaaagaaa aagttcaggt ggaatcctga
    15721 agacatattc ttctcttggg ttttccgtac ttgccagcaa ttgatccaaa tcaatcaggt
    15781 cagattttag atctgcttca attccaaagg ttttttgatc aagcagcagg taggttatta
    15841 tgttcttgaa aaatccattg agcatgaaat cactgttacc caggtttcca gtcacctcat
    15901 tcaaagccag gtcgctgtta ttaaatagaa ggctgccatt cagattctga aaaggtaatt
    15961 tatttccctt ccatttcaca tttacatcca gcatggcgat gtcgccgctc atctcaactc
    16021 tgtcaacagt agatttgttt ttgagatttg agagctttcc ggtaaaatct atattgaact
    16081 gaatagctcc cgaagcttga tcgatacgtt caacaggata aaaggagaaa agagaactca
    16141 tatccatggc gccggtaaaa acagtttcaa tccatggatc tttaaaattg gtgattgtta
    16201 aatctccggt gaaggttttt ccttccagcc agcaaagaat attggtaagc tttaaagttg
    16261 cctgggtggg atcattgaga ttttttgttc gaaattctcc tgtcaattgt acatcggtta
    16321 gccttgattg atactcagga tgaaatatcg cggctttctc agtaccaaaa tttataacaa
    16381 cttccggaga ctttgtggaa gaaataattc cggttagttt tcctttcaaa taaatatctc
    16441 cctcactttg ataccggctc agcctgtttt gattttctaa aggcagaaaa ttaagaattg
    16501 tttggatgtc ggaatctttg gcttcaaaat ctaattccgt gaaggaggta ttgaaatcat
    16561 aatttccagt gataacatag tttcccctgg cattggaaag atcgcattga gcaatgtcaa
    16621 ggaattttgt ttccaggtta taggtgagat cggaattaac tttccaatct tgtgcccgaa
    16681 taaatttatc attttcaaaa cgtaaataat tgcttgtaat atccccgtgc aaccgcgtaa
    16741 taacataatt attcaatatt tgaagggaag catttacaga atgcaggtca agatcgtact
    16801 cgctgtcttt cgcttcatta ataaaattga gatttatccg ggtgagaaaa actttcttaa
    16861 gatccaccac cattccccct gaagattttt ccgcacgttt caggatctga taattgggat
    16921 ttccgttttt gtcaatagcc aatcttactt ttccattttg agcctgtaat tcattaatta
    16981 cgtaatcgcc ctgtatcaaa tccagaattg aaaaagaaac tacgataaga ccggcggttg
    17041 caagtattcc gtgttgagcg gtattctcct caattactac attgtgcaga gcaactgaaa
    17101 ccgacggaaa attcttccaa attgtcgctt caattttttc cgcatgaatg ggagtcagta
    17161 gatgtttatt tccttcgcgg attagctgac caactatttt gtcctggtaa agataaacag
    17221 tgacagtccc ggcgattacc agcacaagca tggtaaagaa aaggtagaaa aagccctttt
    17281 taatgtattt caaaccagtc aatcaaatcc cttacaaaga tagcaaaaat ataacgtgta
    17341 gaagtgcgat actgtatcac ggaatgcaaa atatattata agcgtgggaa ttagcctgag
    17401 gttaagaatt cctgttcaat tttacataaa tcactactct ggatttacct attgaaaaat
    17461 cttcttcatt acgatttctt tatacgattt gccaataggt attttccgtc ctttaatttc
    17521 aatctgatta ccttcgatac tttgcacttt ctttttgttg atgatgaacg atttgtgaac
    17581 tcttaagaat aaatcagccg gcagcgattg ctccaggctt cgcagtgatt ccaaagaaat
    17641 aattttttga gactccagaa cataggtaac atattctcgc aggccttcaa cataaagaat
    17701 atcttcaata ttcaccaggt acttttttgg tcctgatttt atttgcatga agtttggtcc
    17761 tgatttttta atttcttctg atgtgacatt ccctggtttt ccttttctta aattatgaag
    17821 gtctgaagct ttttgtactg cagctacaaa tcgctcgaat gtaaaaggtt ttaacagata
    17881 atccaccaca tccagggtat aaccctccaa ggcataatca ggataggccg tagtaaaaat
    17941 aaccatcggc ttgattcgcc gcattgattt gagaaattca attcctgtca accccggcat
    18001 ttgaatatca agaaaaatca agtctgtatt ttcagaactc aatgtttcca tggcttccag
    18061 gggattctta aaggaattta taagatccag ttggggaagg ttatcaatgt aatctttgag
    18121 caatacgcgg gccggatatt cgtcatcaat tacaatgcac cggagcttca tttaggtttc
    18181 aatttcgagt gaaacagaaa attctttttc ggtctcctca atattaagca cgtattttcc
    18241 gggatacaat aattcaagtc ttttctttac gttttgaatc ccaatgccac cggtggcatc
    18301 ctttgaaaat tcagttggcg gaaagctatt ctttaaaatg aaaagaactt tattgcctga
    18361 agtctgaatt ttcagatcaa tccagctgtt tttgtcatct tctactctgc tatgcttgaa
    18421 actattttca ataaaaggaa taaaaagcat gggttcgata taaagacttc catcaacatt
    18481 ctggaagtcg gcggtaatat taacttcctc ttcgtatttc aatttttgaa aaccgataaa
    18541 gttgatgata taatcgatct ctttaacaag cggtactttc ttttcattag aatcataaat
    18601 cacatacctg agcatttctg ataatttcaa aatcatttgc tgtgcatttt gagaattgct
    18661 gcgcatcagt gaatagatat tattcaaggc attaaacagg aagtgcggat ttatctgaga
    18721 tttcaggaac ttcatttcag attggagatt ctcattaata agtgaaagtt cttgttgttt
    18781 cctccttcgt tcgcggttta tcagccagta ggtagtacta ataaaaagaa ttgctgttga
    18841 tgacaacatg ttgctaatca tccagcggct gaaaatcgga cgcggtccct gaaactgtct
    18901 tcgaggtctg atgctatcaa gtgcttcacg gaaaattcca acttcgctgg tgaaattgaa
    18961 aaaataatat acgccaataa taagggtaag aaggccaaat acataaactg catactttcc
    19021 gcgttccagc aatttcggaa gaataatttc agtattcaga taaaatagta aagcatgcac
    19081 aaaaacaact cttgctgata atgcccaggc atactgagac tcaaatggaa aggacattaa
    19141 ataaaaaaca acaagtccat accctgccca tagcagcagg tggataataa gcgccggaac
    19201 ttttagttgt tttgacatat tgaacaaaag taaagcatat aaaaagccag gttcaaacga
    19261 attcaatcaa ttaccgaaat caatcaacga acagccatta ttaatcatca tgtggatttt
    19321 ttgggaagtt tgttgaatga atgatggctt acgttgaata gtttttatcg tgctaaacca
    19381 aattcggaaa ttagcgtcaa agtatcactg taaagaatcc ggagttatga aaagattaag
    19441 ttattcgttg ataataattt tcagcctgtt tgcttttgat aaattgattg cccaaacccg
    19501 tcaaggcggt ggccaggggt ttaacccggc agaaatgatt gaagcggaaa agaaagccgt
    19561 tttagatcgg ataacaactc ttacagagga tcagaaagaa gtaattgaga taatatatac
    19621 tgactataca gaatcattcg ggaaggctcg cgagaaatcc agtggcgatt ttcaggttat
    19681 gcggacaaaa atagtggaat taagagctga gaaagacaaa tcactgaaag aagttttaac
    19741 agaagatcaa tataaagaat acatagcctt gatggaggaa atacgtcaac ggagaatccg
    19801 gggtaattaa aaaaatgaag aagataatat ttagtttggt tttggttctg agctcatatg
    19861 cagcatgggc tcagaccttt tccctcacgg gaacactgaa agacagcgtt gacaattcgc
    19921 cattaattgg ggccaatata attcttacca gtgtaagaga aacagacaag aagttttatg
    19981 ctgtggccga tattaacggt aatttccagg ttaccggttt ggagaaaacc ttttataaaa
    20041 tggaagttag tttcgttggt tatggaactc tgaaaaaatt tgtgagaata acagatcctg
    20101 taaccacttt aggaaatgtg ttgatgaatg aagatgcaga attgctgcag gaggtaacaa
    20161 tagaagggga aacagtggct tcagttcaaa agggtgatac gactcagttc aatgcgaatg
    20221 cttttaagac caaccccgat gcaaatactg aagatttaat ccggaagatg cccggcatta
    20281 cagttgaaaa cggaaccgtt caggcgcagg gcgaaaatgt aggcaaagtc ctggtagatg
    20341 gacgggagtt ttttggaaat gatgcaacgg ttgctctgcg ttccttgccg gcagatgttg
    20401 tggagaagat tgaagtattc gacaggttaa gcgaccaatc tcaatttacc ggtttcgatg
    20461 acggcaacac caccaagaca ataaacattg ttacaaagtc tacactgaag actagtcagt
    20521 ttggaaagat ttacgcaggc tacggtactg ataacaggta ccttgctggt ggtaatctta
    20581 atatttttaa tggtgatcaa cggatttcat ttattggtat gtccaataat atcagtgaac
    20641 aaaacttttc tacgcaggat ttgctgggtg ttgttggtac aagtaaccaa agacgtggat
    20701 tcggaggtgg cgcgggcggc ggattcaccg ggggccgtcc aggatttaca ggtggtaact
    20761 tccggtttgg cggaacatca aacttcctgg ttggtcagca aaatggaatc acttcaacca
    20821 attctttcgg aatcaatttt atggattcct ggggagaaaa agtgaatatt accgctagct
    20881 attttttcaa tatgaccaat aatgataatc tgcagctggt ttcccaggaa tatttcctgg
    20941 atgacaataa tcaattctat gatgaatcaa caatttcacg ggcggacaat atgaatcatc
    21001 ggctgaatct tagattggag tatacgctaa atgaaagaaa ctctttaatc tggacaccca
    21061 ccgttaattt acaggattat gaatcctctt cacaggtggc aggagttaat tatttagatg
    21121 aaaccaattt acttagcgaa actgaaaatt ttaacagcgc gaacaactac ggatataaca
    21181 ttgcaagtaa tcttctgttg cgtcacagct ttgcaaagcg gggcagaact ttatcatgga
    21241 acattagaac cgattataat gaaaaagacg gggatgcatt tctcacttcg tccaacaatt
    21301 tttacaacgg accggtatat tcaagcgatt cattgctcca gcaaacaatg acaggttccg
    21361 atggctatac ttttgcatcc aatcttgttt atacggaacc gctgggtgaa tcaggtcagt
    21421 tgcagttgaa ttataatact tcttttacaa ggagtaattc cacccgatat acttatgact
    21481 ttaactacag cgatcagctt ttctcagttc tggataccgc tttatcaaac acattcatga
    21541 atgattatct tactcaaaga gcaggtggca gttacaggtt gcgcaaaggc aaggtattct
    21601 tcacgctcgg tgctgatttt caaaatgcaa accttgtggg tgatcaaacg tttccggttg
    21661 aaacaagtgg agaaaaaacc tttaataata ttctaccgaa tgcaatgttt atgttcacag
    21721 tttcaagaaa caccaatttg aggattttct acagaacccg gaccaatgcg cctactatca
    21781 gtcagttaca aaatgttgtt gacaacagca atcccttaca attaacaaca ggtaatccgg
    21841 atttgaaaca gcagatagat catttcgtaa ttgcccggta ttccctgagc agggttgaat
    21901 caaatagtac tttttttgct ttagcatttc tgcgattcgc agataattac ctggggaatt
    21961 ctacgataat tgcggatcaa aacatggttc tcccggacgg cacggttttg aaccgtggca
    22021 gtcagttttc catgccggtt aacctgagcg gatactggag tggacgagga ttcctcactt
    22081 atggctttcc attacgattt gtaaaatcaa acctgaatct aagcagctct tataactatt
    22141 caagaactcc cgggctgata aataacgcgt tgaataattc agattcacat acgtttagcg
    22201 aaggcataac actcagcagt aatatcagcg aaaaaataga tttcaccctt tcatattccg
    22261 ctaattataa tattgtggaa aattcaatcc ggcctgagtt ggataacaac tatttctatc
    22321 atacagcagg atttggttcg gcaatcacat tttggaaagg cttagtgtta cgaaatgatc
    22381 ttaataacta tctgtaccgt ggcttgtcgg atgaattcaa ccaggatttc ttcttatgga
    22441 atataagcct ggcgaagaga atcttcaaag agcaaaacgg agaaataagg ttgggtgtgt
    22501 ttgatgtact gaaacagaac agaagtatta ccagaaatgt tacagaaact tatgtggagg
    22561 atgtgaatac cgaagtattg aaacaatact tcatgttaac cttcacttat aacctgaagg
    22621 cattaggtca gaagaaagga taagattttt tttaaggaca taatcgatat tattcttacc
    22681 ggcgtaatca taatcaattt caccaataac gtcaaagccc tgccgtttgt aaaaacgcag
    22741 agctctcgga cttaagctcc aaacagtgga ataaaagttt tgaaatccac gggattgata
    22801 atgttcactc atcatctgca taaactttgt accaattcct ttgccggtat atttttgcag
    22861 cagatataat ttaaacaatt ccgcggaatt gtcggatctt ttgcggatac tatcttcaat
    22921 caaaataaaa cgccttacct gcgcgaaacc aattgcccgg tcagacttct taaccagaaa
    22981 gtaatcctga tcttgttttg caatttcctt cttcagagtt tcttcggaaa aaaaatcttc
    23041 aagatatggt tttaagtcat tataagtgta ggagccaaag taagcttgaa aataagcaag
    23101 tgttgatact tcatgcaaac tgtgtaaatc agcatcctga accaattcaa atttaatcag
    23161 agacttatcg cttattgaaa ccatcgaggt aaaaatcaaa aagaagcaca acaatccaaa
    23221 agaaatgcca acttttatta atttgttatc aaaacaccaa actcatgaaa actttaggtt
    23281 attattttac actttgtcta ttaattgtta gcgctcaata tgttaacgct caatacaacc
    23341 ttgagcaaac tcttggtgca ccgtatgttc aatctatttt tccttctgaa gacggcaagt
    23401 cattggcctg gatcatcaac gaaaagggaa tcaggaacat ttggtttaca gaagttaatg
    23461 cgacagcacc ccgaaaggta acagcttatg accgggatga tggtcaggat ttgggaaaca
    23521 tcactattac aagtggattt ctgatcttca caaaaggagg tggaagcaac aggtatggcg
    23581 atattgccaa tcccgcatct cttcctcagg ttcctaaaag agaaatcctg aggctaaatt
    23641 tgtctaatga ctcaatttca aaagtggatg aagcagcagt agcggtaatt gctcccgacg
    23701 gcaaaagaat gttagtgaca aagcaatcaa gggtttttct tattgaagat atttctgcac
    23761 cggaaattaa gtcagaagtt ttgtttgaag tcagaagaag tgcaggctcc ttgagctggt
    23821 cacctgacgg aaataaaatc gcatttgtca gtttcaggag tgatcacagc ttcgttggag
    23881 tctactcaat agaagaaaat aagatcacct ggctggcgcc atccttgttt tttgattctg
    23941 atccggcctg gtcacctgat ggaagtaaaa ttgcttttat ccggacgccc ggagcgggga
    24001 ggggagaact acgtaatatc atgagtggag ttcaatttga aattgtagtt gccaatgtca
    24061 actcaggaac aggtaaaaca atctggaagt caccgggaaa ggatggcggt tactcacaat
    24121 actaccccaa tgcgcccttg cgctggacta aagaaaataa gatacttttt tattcggaac
    24181 atgaaggctg gaatcatatc tattccataa cccctgaggg cagcgggctc aaagatcttt
    24241 cacccggcga atgtgaagta gagaacagtg cgttgtctgc caaccatagg tatttgtatt
    24301 ttgacagtaa ttgtaccgac ataaaccgac ggcatatctg gaaagtaacc gtggcgaccg
    24361 gccaatccca aatggttact gacaaatccg gtattcaatc caatgtggta acccttgagg
    24421 gcgaagcaat tgcatatcgg gaggggaggc ataatattcc gacaactgcg ttgattcaaa
    24481 atgggagtac aactacgaac ttagtctccc tgccggataa ttttcccgct tcgcaattag
    24541 tgatgccgga ggcggtacaa ttcaaatccg cggatggact cactattcac ggtcagttgt
    24601 ttttgccttc gaaacttgat aagaccgcca aactaccggc tgtaatcttt atgcacggcg
    24661 gacctattcg tcagatgttg ttaggctacc attacagcgg gtattatacc aatgcctata
    24721 tcctgaatca atatatggct tccatcgggt attcagtttt atctgtaaac tacaggaatg
    24781 gtattggata cggacgggat ttccggttgg atgaaaacca gggacccaga ggtgcatcgg
    24841 aatataagga cattattgca gcgggcaaat ttctgcaatc aaggccggag attaaccccg
    24901 aaaaaatcgg tttgtggggc gggtcttatg gcggttacct gactgcaatg ggcctggcaa
    24961 agaactctga tttatttaaa gccggggttg atttacacgg agttcatgac tgggcatggc
    25021 ggggtgaaga attttctccg ggtggaggct ggggaataac cccagaactt atggaacttg
    25081 cttatcaatc gtcgccgatt tcagaaatgc aattctggag ttcaccggta ttaataatac
    25141 acggagatga tgaccggaat gtaatgtttg gccaatccat agatttgcaa aagaaactgg
    25201 atgaattggg agttcacaat gaaatcctgg tatttcctga tgaggttcat ggctttctga
    25261 ggtatgaaag ctggctgcgt gccaataagg cgacggtcga tttcttcaac cggttcttaa
    25321 agtaactaac cgctgcaggt aaaggcagag gtttcgcagg caacaatggt tactgttccc
    25381 ggagaacatc cggcgccgcc gctaatgcct tcaaaaatga aagcatgaac accggtaccc
    25441 aaatcaatct ctatggattc tcctggatct aacgtgccat gatttacacc gtctaccagg
    25501 gaacgcatga cggtgctgct tgaggtgttt gttaatttga gtgttccttt gcttttcaga
    25561 agacaatttt ccttgcacga aatcaggaat attattagaa taaatacccc aagcaaagtt
    25621 ttcaaaattt tcattttggt agaattttat agtttaaaaa taaggaatct gactcattaa
    25681 tggcaagggg agtagcaggt ttattttgga ggtaaataca ataataccaa tctttataga
    25741 ccaattggga ctatttttga gttaataaaa ttatgaagtg gctcaggtta ttatggatat
    25801 ttccctttct gctaatcctc tcatttggca ggccggacaa acaccagctc tacaaccggt
    25861 gggataatat tcattttgtg aaagaaattg attttgaccc ccacccggat accgtgtttg
    25921 tgatttgtac caaccgtctt taccagcccg gcttgcaaca accacttgca gatagagctg
    25981 atcctgaaaa taaacttcaa tacctggttg caacttattc aaacaatgaa tggagactgt
    26041 tgcggaaaca aaatcttgaa gaagcggttg cttatcttcc aaggaaagat ttcttgcttt
    26101 acatagaagg aatgggaaaa acttttccca tggatttgca gcgatcagcc ggaatgaccg
    26161 ctcaatacaa cttaaatgta attcagtttg aatatcccag catccatccg cgaaagaacc
    26221 cggttgctaa tttttatttc gcttacaacc aggccaaaca tgcaacagaa gaatattcag
    26281 aatttattcg cgagttcgag gtgttgaaaa ataatcaccc ggaaaaattt gccgaagtaa
    26341 atgtcagttt attccatcat agtatgggca accgaatgct aaagcattct ttggaagaaa
    26401 atttgctgat agtaaaagac aaatcattat ttcagaatgt tattctgaac gcggcctgcg
    26461 tacctcaaag taagcacacg gaatggattg aaaagattga tttttcagaa agagttttta
    26521 tcaactacaa taaaaaagat tttcagctga acggggcaat gttcattacc tttgataaga
    26581 tgctgggagc gcggttcaaa gaaccccttg ccgggaatgc cacgtatatt gattttcaaa
    26641 ctttggtggc tggccggcac agcaattttc tgaacattga tggccgggat aacatcaacc
    26701 ctgagtgccg gcgatattat gatctcgtgc ttcacggcaa caatccaaat ttggcagact
    26761 ccagtttatt tataaccagc acagaaattg ttgggtttgg attgaaataa tcaggcaggc
    26821 ttttgcttta aaaaagcggg aagcagcctg attggtagca gcctctggat tccgcgcgag
    26881 cgggacaagc aggggttaat aatttgtgaa aggactttgt caatcgaata aatttctcct
    26941 caatacctaa tcgtatattc cactgtgtcc acactcacca gctctatatc atgggaataa
    27001 ggggtagtta taccattttc ggttacccac caggtaaggt tgatggtagc gttactttcg
    27061 aaggtataat tgcgaaatac attttcatta caggggccaa ggcaatacaa acattcttta
    27121 tcatttgaca ataaatgttc tccatccata caaaaataaa tggagtctga atcattgtat
    27181 ggtatgtcat tgactaaatt caatgtgaat tcagcagtcg gaaccaaata aacattcaaa
    27241 gtattttcct ctcctgattt gacaggttca ttgcattgat tgcaataata tccttgtctt
    27301 ccaattgcaa tcttgtaccc atttccgcgg tcggcttcaa attttatttc gtaatagcca
    27361 tttgaatcac ttatgttatc aaaatgagta attgtttctt ccggcttcag gaaaggctta
    27421 aaacttagat atgcacccgc tccctcaatg ggcttaccag atctgatttc acggatatat
    27481 ccttttacaa tggttgtatt gtcttcaaca atatgaatac aggaggcaaa tatcaaactt
    27541 gcaatcaaaa acaagattcc attctttatc atctcactat tattaaccgt ttattttcaa
    27601 tgccttccct gctctccagc gaaataaggt aaattccggt tttaagctgg gtaacatcca
    27661 attcatactt ataggttccg gattcaatag tatacccgcc cagaatttct tttattaaat
    27721 tgccttccag ggcaagtaat ttcaaagaaa taatgccagg tggaatgtcc ctgatttcaa
    27781 tcatagtttt gtctgccgcc ggatttgggc ttagttccag gtgatcagca aattcctgca
    27841 caagcttttg ctgagattgt ccgcaggcct catcattgtc tgacaaaact tcaaaagcaa
    27901 gactaacctg ggtttgttga aacatccggt caagctcatc ctacaggcta acctcacaat
    27961 ggatcacatg gccgccctgg tccggggata ttggtaattg gtaataggaa gcctccgttc
    28021 tttccgtatc attgtcaagc caccagctgg tagaaattct ttttaccaac ccggaattcc
    28081 ggagggtaaa catatcctgg gccaggccgg ggcagtaggt ttcaggacct tcaatagacc
    28141 ttttattacc attcatcctt ctaataccta atcgtatatt ccgctgtatc cagaccaacc
    28201 aattcaacat agtgagaata aggagttgtt atgctatttt cagttaccca ccaggtgaga
    28261 actataatgg cattgctttc aaacgtaaat ttgttaaaaa cattttcatt acaggggcca
    28321 aggcaatacc aacagtcttt ttgggtagat gataggaatt ctccatccat gcaaaaatat
    28381 attgagtcag cctgagttga gggaggctcg ttgataagat taagtgtaaa ttcagctgtt
    28441 ggaaccaaat aaacattcaa agtattttcc tctcctgatt tgacaggttc attgcattga
    28501 ttgcaataat atccttgtct tccaattgcc actttatacc ccttgccccg gtccgcttca
    28561 aattttattt cgtaatagcc atttgaatca cttatgttat caaaatgagt aatggtttct
    28621 tctggtttca gaaagggttt aaaactcaga tagcaccctg ctccttcaat gggtttgcct
    28681 gacctgattt cacggatata tccttttaca atggttgtat tgtcttcaac aatatgaata
    28741 caagaggcaa atatcaaacc tgcaatcaaa aacaaaattc cattctttat catctcacta
    28801 tcattaag
//
LOCUS       DASWHX010000131        17733 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_1985577, whole genome shotgun sequence.
ACCESSION   DASWHX010000131 DASWHX010000000
VERSION     DASWHX010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 17733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17733
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1985577"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..683)
                     /locus_tag="VGA21_09005"
     CDS             complement(<1..683)
                     /locus_tag="VGA21_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019597246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEX9650687.1"
                     /translation="MGCTSPEKGESLVNSEIEDNLSKLKLPSGFEISIFAEVNNARSL
                     AISPSGTVFVGNRNGGKVYAVKDNNGDGKADVKYELASGLRMPNGVAFKDGDLYIADI
                     STIYRFRNIESKLSNPGQPEVVYDEYPSDRHHGWKFIAFGPDGKLYVPVGAPCNICES
                     KDEVYASITRINPDGTGREVYAHGIRNTVGFDWHPGTGELWFTDNGRDMLGDDIPPCE
                     LNKVSVQGQ"
     gene            825..2735
                     /locus_tag="VGA21_09010"
     CDS             825..2735
                     /locus_tag="VGA21_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008630289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEX9650688.1"
                     /translation="MLSIQNLSYFIGNRPLYEDASLHIGDRDKVGLIGLNGTGKSTLL
                     RLIYGEYNAQAGSISKSKNSTLGFLNQDQLSYQTGDSILTVVMQGFKEAVEIQKKIEH
                     ILHEMENNYSDKLVEKLTKLQERFEALHGYSLQSKAEEILEGIGFSTGDLHRPLKEFS
                     GGWRMRVMLAKLLLEKPSLLLLDEPTNHLDLPSIEWVENYLNNYEGSYIIVSHDRRFL
                     NACVTRTVEISQRKLWVYEGNYDFYLKEKALREEIQKNAFENQQQKIKQTEKFINRFR
                     AKASKARQVQSRIKSLEKMDIVEDIVESTSAINFRFEFKQKPGRFLCRLLDIEKHYES
                     NEVIKDTTLFIERGDKIALVGANGKGKSTLLRIIAGSESHGGKREDGYNVITAFYAQH
                     QIEALHVENEILDELKMAGSGKSELELRNLLGCFLFTGDDVYKKIKVLSGGEKSRVAL
                     AKTLTSEANFLLLDEPTNHLDFISENILIQALQQFKGTFVAVSHNRYFMSQIANKIWY
                     IDNKQIKEYPGTYDEFSYWYKKQIESKQQGLEKAKANAAPPKKEVRESMKPKVFDEQK
                     ELEKEIKILEGNIAGLETEKQKLEDKLTNPEVYGNPDKLQEINSLYKEVEVSLAGYNS
                     KWDALISQLTES"
     gene            complement(2740..3483)
                     /locus_tag="VGA21_09015"
     CDS             complement(2740..3483)
                     /locus_tag="VGA21_09015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650689.1"
                     /translation="MMRLLTFVFIFICSQHFVVAQNKYHDVIRLKNGSIIKGTIIERK
                     PNESVTILIGGRESITFFEEEISRIDKEGRLKFRDSPHRKGYFTRTEMGASIRKNHEF
                     QGSYHTILPWFQTIHGYKWNRYLETGAGLGTDFYESVITVPFFIHVSGELLNSKVTPI
                     YLIQYGDSFILNGKQIQLWNDPDVEAKNFFRIATGFQVKNPANAFYITAGFKLNKVVY
                     KDDPWWWDSATTITTRLYRSFTFGFGMKF"
     gene            complement(3484..4794)
                     /locus_tag="VGA21_09020"
     CDS             complement(3484..4794)
                     /locus_tag="VGA21_09020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650690.1"
                     /translation="MKRSRLISDKLIIALEFVFFLLLLISASSLLQSCEDKCETVTSY
                     IMMEPVYKTTEEVRNGFGIKEPHPIESSGKIYVFGEYLLVNEPGNGIHIIRNADCNPI
                     PVSFIAIPGNFDMAVKDYILYADSYVDLLAIDISNLPEITLIKRLENAFPNYNYEWGV
                     GYGGIDNLIVTHFEEKEIVDIQTDCNNRANFWERNVLFIGSCVNCSAPQLAGNTPSGV
                     GGSMARFAVSGENLYAVDFNSLRVFDISTQTDPQAGNVVEIGWGIETIFPYKDNLFIG
                     SNSGMFIFDNSDPTSPYLKSQFKHVRTCDPVVVDDRYAYVTLRSGSECEGFTNQLDVL
                     DIADLSNPFLLKTYPMQNPHGLSIDGDILFITEGDFGMKVFNAADRNDIDNHLLDAIT
                     NVHGFDVIAFNQKAILIGEDGLYIYNYSDPTNLETCGFISVGEK"
     gene            complement(4847..6202)
                     /locus_tag="VGA21_09025"
     CDS             complement(4847..6202)
                     /locus_tag="VGA21_09025"
                     /inference="COORDINATES: protein motif:HMM:NF024957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEX9650691.1"
                     /translation="MKEHKYQFENEWRSAFSQAEAMPSHGVWERIDFELNKKDSKKDN
                     RNLIIWQMVAAVSLALASGVLLFNYFNQPRSFEESILSKAPEQTQESILHGKKDREVT
                     GAEEQNTTITESETDKTIVKRYIEIIPRAKDSKPGIIASTEMQDVAIGFVSPLKYEKL
                     DKVGEIRLRRAWLPTPDVHKIAYSRPLPAEPLKSRNSYPFWAGIKVAGGASGSGESSL
                     SPLALSLRSQSETFNTSPNAFGSSFVQISSSTGFGYSLGFSVGKNIGQRIFIQSGLQY
                     VVQNINGSTNAYFRRLDTQEMEPVFIFNADKPLYSGGTQIRQIEFYSKPIRLISSLEY
                     FSIPLQAGYLIVDRRIDWGIAAGFSTDFFLRDIVTMESGEIDGKVTRNKDEESPFRPV
                     GLSAILSTEVNYNLTDNYSISLEPRYSVGLNPVTRKEAGVFEERRLKSLTLGVKLRYI
                     F"
     gene            complement(6189..6728)
                     /locus_tag="VGA21_09030"
     CDS             complement(6189..6728)
                     /locus_tag="VGA21_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020532358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX9650692.1"
                     /translation="MNEEELIEGCCRNDRKAQEYLYNRYSGKLYVIALRYTKSQSEAE
                     DVIQDSFIKVFDKIREFKRKSKLETWLTRIVINTALNSQRSKLYMFPMADVNEINLED
                     EISLARFEWQDLLKMIQSLPSGCQAIFNLFAIEGYNHNEIAEMLSISEGTSKSQYSRA
                     KILLKELISKNEMVRYERA"
     gene            6846..8123
                     /locus_tag="VGA21_09035"
     CDS             6846..8123
                     /locus_tag="VGA21_09035"
                     /inference="COORDINATES: protein motif:HMM:NF028426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system plug protein
                     domain-containing protein"
                     /protein_id="HEX9650693.1"
                     /translation="MKANLNITLVFLLLSCVPVPYSSTQSGGKDLLFRDFNYEASIKS
                     VLLYPDDNIGVPNLQPGTLSLLSQSSLVLEFDYLSETPESFYARIYHCTKDWKKSNLS
                     DLEFLQVYNEFNLNDYAYSIDTKVDYIHYRFQLPKVKLPGNYVLIIYRNNPDDFILSR
                     RFLVFNSRIQISLAGNLNTINPVLMSDHLIEFTLNYQSIPLDDPMGNISVDIRQNQQW
                     ENYIHDLKANFVREDLKELEFRYFNLENAFKASNEFRFFDLRSVLNFGQNVENVINTG
                     EGFTAILEKEKPRSVQAYGHLNDLNGKYTLENYDRRNPLTGSEYVNILFTLESEKIIG
                     DVYITGELTNWGQTSPMAYNEELKAYTDNLLLKQGWYNYLFKVDSPTLPSYYLEGSHA
                     QTENEYEILVYYRDIMKRADLLLGYKRFIINSR"
     gene            complement(8120..9217)
                     /gene="ychF"
                     /locus_tag="VGA21_09040"
     CDS             complement(8120..9217)
                     /gene="ychF"
                     /locus_tag="VGA21_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010608224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEX9650694.1"
                     /translation="MALRSGIVGLPNVGKSTLFNAVSSAKAEAANFPFCTIEPNVGIV
                     SVKDERLDILKELVHPEKVIPTTIEFVDIAGLVKGASKGEGLGNQFLANIREVDAIIH
                     VVRCFENENIVHVDGSIDPIRDKEIIDTELQLKDLETIDKRISKVEKIAKSGDKSALK
                     ELDIFKRLKIHFEQGKSARTIAWDINEREFVKGLQLLTSKPVLYVANVDEASVLKGNS
                     YVEKLIQAITGEEAEIVLISAAIEAQIAELEDGEERLLFLQEYGLKESGLDRLIKATY
                     KLLDLISYFTAGPKEVRAWTIKKGWKAPKAAGVIHSDFEKGFIKAEVIKLEDYQKYKT
                     EASCREAGKLAIEGKEYVVKDGDIMHFRFNV"
     gene            complement(9226..10380)
                     /locus_tag="VGA21_09045"
     CDS             complement(9226..10380)
                     /locus_tag="VGA21_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013455010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX9650695.1"
                     /translation="MHTRAFLLSIKSMQVNRTIIYLILSILIFVLLAWIFTDILAYFI
                     ISIVIAALLKPVVEKLSKTQFLKIRLPRAFAVFLSFVIFSGVISLFFTLFIPVVSAQI
                     DVLSSLDYENIFNKISEPLTRIENFLIDYNLTENANGFILEELRNSMVNLVKELKIQN
                     ILNTFLSLTGNLFIGGMAILFITFFLLYETTTIRKRMLSLIPNAYFEVTISAFNKIEK
                     LFSNYLLGLLVQMFAIFSVASIGLTLVGVNYALTIAFFAAVANLIPYLGPLLGSLFGI
                     FVAISTQLGNLTPQENIFLVVKIAVVFAVVQIMDNLIFQPLIFSKSLRAHPLEIFVVI
                     FAGAALGRLIVGGLLGGVLGMISAIPFYTILRVSGQELYSGYRQYKVFRN"
     gene            complement(10413..10766)
                     /locus_tag="VGA21_09050"
     CDS             complement(10413..10766)
                     /locus_tag="VGA21_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009582247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3276 family protein"
                     /protein_id="HEX9650696.1"
                     /translation="MEEQNGNDRQEIFSERVRAGKRTYFFDVKSTRSNDYYLTITESK
                     RRYKDDGYTYEKHKIFLYKEDFNKFIAALNSTVNHVKVELLPEVDFAQFDREEEPEKE
                     KEKAESGIDSELKWE"
     gene            10950..11855
                     /locus_tag="VGA21_09055"
     CDS             10950..11855
                     /locus_tag="VGA21_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009582246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX9650697.1"
                     /translation="MKIDLNEIRNFANLELLAKQLVEGFITGLHKSPYHGFSVEFAEH
                     RLYNTGESTRHIDWKVFAKTDRLYVKRYEEETNLRCQIVIDNSSSMHYPVANRGKLLF
                     SVLAAASLAYLLQKQRDAVGITTFSDKIDLQTQIKSTSAHLNKLFIELEHLLTENIKL
                     KTTNVAPVLHEIADKNHKRSLVIILSDMFDRDTELDEIFKALQHLKHNNHEVLLFHVT
                     DQQTEYRFEFEDRPYEFIDLESGEKLKINPTQVKDMYTRKVGEYYNELKLRCGQLKID
                     FIEADIRDGFNNVLKSYLIKRSRMK"
     gene            complement(11857..12207)
                     /locus_tag="VGA21_09060"
     CDS             complement(11857..12207)
                     /locus_tag="VGA21_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010608221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX9650698.1"
                     /translation="MAIKITEECINCGACEPECPNNAIYEAGIEWNFATGTTLKGTIQ
                     GMNGSTVDAESGRLPVSDEFYYIVPEKCTECKGFHDEPQCAAVCPVDCCLPDLAKEET
                     EEELLNKKAWMHGH"
     gene            12417..13412
                     /locus_tag="VGA21_09065"
     CDS             12417..13412
                     /locus_tag="VGA21_09065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA reductase"
                     /protein_id="HEX9650699.1"
                     /translation="MDKEVFETFIILGEKISKNILRIPFKNTQKNNPWFDETSVSAAL
                     HGISIYLEKDNMTNWLKDYSFKKSRKTIGLIAAGNIPLVGFHDLLCILISGFKVEIKP
                     SSKDLPLTKWIIRQITNINPALQTRISLKEKLGNCDAYIATGSNNTNRYFEYEFRSKP
                     HLLRNNRTGIAILTGKETQRDLNGLAADIFQYYGLGCRNVSLLLLPMGYKLVKISKCF
                     GQHQKVMDNKNYSDIYNYNKALLILGKKKFHDLGNLLLVPNNNPNATVSVLHYDFYKN
                     PNHLNSFIRRNRELLQCIAGHRISGISTIPFGTTQTPEAWDYNDGKNTLEFLCNL"
     gene            13517..15787
                     /locus_tag="VGA21_09070"
     CDS             13517..15787
                     /locus_tag="VGA21_09070"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020526592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HEX9650700.1"
                     /translation="MAFDIDMIKLVYTTFGERVHAARNLLGKPLTLSEKILYAHLFDG
                     TPTQVYERGKSYVNFAPDRVAMQDATAQMALLQFMQAGKKNVAVPSTVHCDHLILAKD
                     GAKADLKNSITASKEVFDFLESVSKKYGIGFWRPGAGIIHQVVLENYAFPGAMMIGTD
                     SHTVNAGGLGMIAIGVGGADAVDVMAGMAWELKMPRLIGVKLTGKLSGWTASKDVILK
                     VAGILTVKGGTGAIVEYFGEGAQSLSCTGKGTICNMGAEIGATTSTFGYDDMMETYLR
                     GTGRNDVADLANKIRAHLTGDDEVYANPDKYFDQVIEINLSKLEPHINGPYTPDRATP
                     ISQFAEEVKKNGWPQKLEVGLIGSCTNSSYEDISRAASIAEQALAKNLKVKSEYTVTP
                     GSEQVRYTVERDGFLQKFEQIGGVILANACGPCIGQWSRHTTDPDRKNSIITSFNRNF
                     SKRNDGNPNTHSFVASPEIVTAMAIAGDLTFNPLTDKLKNENGEDVMLDEPHGIQMPP
                     TGFAVEDAGYQAPAEDGSGIEVIVKPDSKRLQLLAPFEPWDGNNITGIRLLLKAKGKC
                     TTDHISMAGPWLRFRGHLDNISDNSFTGAVNYFNDKADSVKNLLTGEYGPIPATQRAY
                     KAKGIPTMVVGDHNYGEGSSREHAAMQPRHLGVKAILVKSFARIHETNLKKQGMLALT
                     FANENDYDKILEDDIIDFKDLPDFTPDAPLTLVLKHSDGNQEEIKVNHSYNNFQIEWF
                     KAGSALNLIKKKEAGI"
     gene            15827..16519
                     /locus_tag="VGA21_09075"
     CDS             15827..16519
                     /locus_tag="VGA21_09075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650701.1"
                     /translation="MMKRILWIILIAPPVLAFMAKPSTFEIKVLGTYTDAIAGNSKER
                     IDLKSLDLKRESFGIGLDADSRAEILIYKGKIFKSSFNNKGIITEKTNNSTASYIVYT
                     SVRKWESYLLPSTIRSLADLETEIAKVIENNNYTGSGTIPFLLIGNPISANWHIEVTP
                     GTNKQLNNRGFFFEDKIKAVGFFTTDQQGVITEMNSRIHVHVTNKRKEIVGHLDDIKM
                     AGGMTLWMPKLK"
     gene            16600..17172
                     /locus_tag="VGA21_09080"
     CDS             16600..17172
                     /locus_tag="VGA21_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018474289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HEX9650702.1"
                     /translation="MAKKKNTAKSKQVITAKRSITKETENLLNTQIIMEGTSSAYYLS
                     MASWCHTKGYDYSSRFLYRHSEEERMHMLKLFKYINDAGGHALQPEITNIHHTFKSLR
                     EVFNLILGHEIEVTKSINKIVDHCFSIKDFTTFNFLQWYVTEQREEETLARRAVELFD
                     LIGEAGIGLWTIDQEIGKLDSFAANSAPEN"
     gene            complement(17176..>17733)
                     /locus_tag="VGA21_09085"
     CDS             complement(17176..>17733)
                     /locus_tag="VGA21_09085"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HEX9650703.1"
                     /translation="RTENSRFNYSEIKGYKESSVLVLIFPQNGSAYIPLIKRPDYDGI
                     HSGQISLPGGKREVQDKNLVDTALRETWEEIGAQINQIKILGSLTPLYIPPSNFMVNP
                     FVGFVESKPQFKIDPVEVQKIIEAPVSYFVEGNNVLNKEIEIIRKSGKANEVIKLETP
                     YFDLEGEVLWGATAMILSELKELMR"
BASE COUNT         5554 a         3620 c         3371 g         5188 t
ORIGIN      
        1 tgctgaccct gaactgacac cttattcaat tcacagggcg gtatatcatc tcctagcata
       61 tcccggccat tgtcggtaaa ccacaattct cccgtgccgg gatgccagtc aaaccctacc
      121 gtatttctaa tcccatgcgc atacacctcc ctgccggttc catcaggatt tatccgggta
      181 atacttgcat acacttcatc tttgctttca catatattac agggagcacc aaccggaaca
      241 tataatttac catcagggcc aaaggcaata aatttccaac catgatggcg atcactggga
      301 tattcatcat aaacaacttc aggctgtcct gggtttgaaa gcttactttc aatatttctg
      361 aacctgtaaa tagtgcttat atcagcaatg taaagatcac catctttaaa agccactcca
      421 ttcggcattc ttaatcctga cgcaagctca tattttacat cagccttgcc atcgccatta
      481 ttatctttta ctgcatatac tttaccgccg ttgcggtttc ccacgaaaac tgttcctgaa
      541 ggactaatgg caagtgacct tgcattgttt acctcggcaa aaatggagat ttcaaatccg
      601 gaaggcagtt taagcttcga aaggttgtct tcaatttcag agttgacaag tgactcacct
      661 ttctcaggag acgtgcaccc catgctgaaa agcataaaaa ccgaaaggga aaatgatatt
      721 aacataaatg gtacagctta gtttcaacct aaaatttaca agaatctaat aagtaataaa
      781 gacaaactca ctaatttttg atgaatggaa tctaaatttg caaaatgctt tctattcaaa
      841 acctgtcgta ctttatagga aaccgcccct tatacgagga tgcctcgctt catattggtg
      901 accgggacaa ggtaggtttg attggcctga acggaacagg aaaatctact cttttgcgtt
      961 tgatttatgg cgaatacaat gcacaggcag gcagtatcag caaaagtaaa aacagcactt
     1021 taggtttcct taaccaggac cagttatcct accaaaccgg agattcgatc ctaaccgttg
     1081 ttatgcaggg ctttaaagaa gctgtagaaa ttcaaaaaaa gattgaacat attctccatg
     1141 aaatggagaa taattattca gacaaacttg tcgagaagct aaccaaactc caggaacggt
     1201 ttgaagcgct tcatggctat tcattgcaat ccaaagcgga agagatcctg gagggaattg
     1261 gattttcaac cggggatttg catcgtcctt taaaagagtt ttccggagga tggcgcatgc
     1321 gggtgatgct ggctaaactt ttgttggaaa agccttcact cctgctgctg gatgagccga
     1381 ccaaccattt ggaccttccg tccattgaat gggtggaaaa ttatttaaac aattatgaag
     1441 gttcttacat aattgtctcg cacgaccggc ggtttcttaa cgcctgcgta acccgaacgg
     1501 ttgaaatcag ccagagaaaa ctttgggttt atgaagggaa ttatgatttc tatttgaaag
     1561 aaaaagcgct gcgggaagaa attcagaaaa atgcttttga aaatcaacaa cagaagataa
     1621 aacaaactga aaaattcatt aaccgctttc gggcgaaagc ttcaaaagcc cggcaagttc
     1681 aatccagaat taaatctctg gagaagatgg atattgtgga agatatagta gaatccacct
     1741 ccgcaattaa tttcagattt gaatttaaac agaaacccgg tcgatttttg tgcagactgc
     1801 ttgatattga gaaacactat gagagtaatg aagtgataaa ggatacaact ttatttattg
     1861 aacgcggaga taaaattgcc ttggtaggcg caaatggaaa aggaaaatca acattactta
     1921 gaatcattgc cggaagtgaa tcacatggag gtaaaaggga agatggatat aatgtaatca
     1981 cagcctttta cgcgcaacat caaattgaag ctttacatgt ggagaatgaa attctggatg
     2041 aattgaaaat ggcgggttcc ggtaaatcag aattggaatt gagaaatctc ctgggctgct
     2101 ttctttttac cggagatgat gtttataaga aaataaaagt attgtccgga ggtgaaaaat
     2161 caagggtggc attggccaag acattaacca gtgaagctaa tttcttattg ttggatgaac
     2221 ctaccaatca cctggatttt atcagtgaaa atattcttat ccaggccttg cagcaattta
     2281 aaggaacttt tgtggcggtc tcccacaaca ggtattttat gtctcaaatt gccaacaaaa
     2341 tttggtatat cgacaacaaa caaataaaag agtatcccgg cacatatgac gagtttagtt
     2401 attggtataa gaaacagatt gaaagcaaac agcaaggttt agaaaaggca aaagctaatg
     2461 cagctccacc aaaaaaggag gtccgggaat caatgaagcc taaagtcttt gacgagcaaa
     2521 aggagctgga aaaggaaatc aaaattttgg aaggtaacat tgcgggactg gaaactgaaa
     2581 agcaaaaatt agaggataag ttaactaatc cggaggttta tggaaacccg gataaattgc
     2641 aagaaatcaa ttcattatat aaagaggtag aagtctccct ggccggttat aattcaaaat
     2701 gggatgccct gataagccag cttaccgaaa gttgaaggct cagaatttca ttccaaatcc
     2761 aaaggtaaaa ctcctatata gccgagtggt aatggtggtt gcggaatccc accaccatgg
     2821 atcatcctta taaacgactt tatttaattt aaatccggca gtaatataaa aggcatttgc
     2881 cggatttttc acctggaaac cggtggcaat cctgaagaaa ttctttgctt caacgtccgg
     2941 atcgttccat agctggatct gcttcccgtt cagaataaaa ctatcaccat attgaatcag
     3001 gtaaatcgga gttactttcg aattcaataa ttcacccgaa acgtgaataa agaatggaac
     3061 ggttattacc gactcataaa agtcggttcc aagaccggca ccggtttcca ggtacctgtt
     3121 ccatttatat ccatgaatag tttggaacca gggaaggatt gtatgatatg atccctgaaa
     3181 ttcatgattt tttctgattg aagcgcccat ttcagttcgg gtgaaatacc ctttcctgtg
     3241 aggtgagtcc ctgaatttca atcttccttc tttatcaatt cgtgaaattt cttcttcaaa
     3301 gaaagttatg ctttcccggc cgccaattaa aattgttaca gattcattcg gttttctttc
     3361 gataatggtt cctttaatga tacttccgtt ctttaatctt ataacatcat ggtacttgtt
     3421 ttgtgccacc acaaaatgtt gcgaacagat aaaaataaaa acaaaagtaa gaagtctcat
     3481 catttacttt tctccaacag aaataaatcc acaggtttca agatttgtcg gatctgaata
     3541 attgtatata tacaatccat cttctccaat caaaatggct ttttgattaa atgctatcac
     3601 atcaaaccca tgaacattag taatcgcatc caacaaatga ttatcaatat catttctgtc
     3661 tgcggcatta aaaactttca tcccaaaatc cccttcagta atgaacaaaa tatcgccatc
     3721 aatactcaaa ccatgaggat tttgcatcgg ataagttttt agcaggaatg gattggaaag
     3781 gtcagcaata tccagcacat ccagttgatt ggtaaagcct tcacattctg atcccgagcg
     3841 aagagtaaca tacgcgtaac ggtcatcaac tactacgggg tcgcaggttc tgacatgttt
     3901 aaactgagat ttcaagtacg gagaagtggg atcactatta tcaaagatga acattccgga
     3961 attactgcca atgaataaat tatccttata aggaaaaatt gtttctatcc cccaaccaat
     4021 ctcaactaca tttcctgcct gcggatctgt ttgcgttgag atatcaaaca cccgaaggga
     4081 attaaaatca acggcatata aattttctcc tgataccgcg aacctggcca ttgagcctcc
     4141 tacacctgat ggagtgttcc ctgccaattg tggtgcggaa caatttacac aactaccgat
     4201 aaagaggacg tttctctccc aaaaattcgc cctattattg cagtcagtct ggatatccac
     4261 tatttctttc tcttcaaaat gagtgacaat caaattatca attccaccat accctactcc
     4321 ccactcataa ttataattcg gaaaggcatt ttcaagccgc ttgatcaatg tgatctcagg
     4381 aagattcgat atatcaatgg ctagcagatc aacatagctg tcggcataca ggatatagtc
     4441 tttaacagcc atgtcgaaat tacctggtat cgcaataaaa gaaaccggga tgggattaca
     4501 atccgcattt ctgataatgt gaatcccatt accaggttca tttacaagca ggtattcccc
     4561 aaatacatag atttttcccg aggattctat ggggtgtggc tcctttatgc caaatccatt
     4621 tcttacttct tcagtagttt tataaaccgg ttccatcatt atataagatg ttaccgtttc
     4681 acatttgtcc tcgcaacttt gcaacaggga acttgcacta atcaatagta acagaaagaa
     4741 aacaaactca agggcaataa taagtttgtc ggaaattagt ctggatcgtt tcatactctg
     4801 agtcttttcc acttagaccc cagggaagag cgaatggttg gaaaagctag aaaatataac
     4861 gtaattttac tcccaacgtc agcgatttga gcctcctctc ttcaaaaaca cccgcttctt
     4921 tcctcgtaac gggatttaat cctactgaat accggggttc cagggaaata ctataattat
     4981 ctgtgaggtt gtaattcact tcagtagata gaatggcgct caatcctacc ggtctgaatg
     5041 gagattcttc atctttattt cgcgtcacct tgccgtcaat ttctcccgat tccatcgtta
     5101 cgatatctct caaaaagaaa tcagtagaaa atccagcagc aattccccaa tcaattctcc
     5161 ggtcaacaat aagatacccg gcctgcaaag gaattgaaaa atattccagg gaacttatta
     5221 aacgtattgg cttggaataa aattctatct gtcttatttg agttcctccc gaatagagcg
     5281 gcttatcagc attaaatata aagaccggct ccatttcctg cgtatcaaga cggcggaaat
     5341 aggcatttgt tgaaccatta atattttgaa caacatattg taaacccgat tgaatgaaaa
     5401 ttcgctgacc tatatttttc cctactgaaa atccaaggga gtatccaaag cctgtggaag
     5461 atgaaatttg cacaaaggat gaaccaaatg cattgggtga agtgttgaat gtttccgatt
     5521 gagacctcaa ggagagagct aacggtgata gcgatgactc cccggaaccg gaagctccac
     5581 cggcaacttt tatcccagcc caaaacggat aactatttct tgatttcagt ggttctgccg
     5641 gcaaaggcct tgagtatgct atcttgtgca catcaggagt tggcaaccag gctcgtctta
     5701 atcttatttc tccaacctta tccagctttt catactttaa tggagataca aaaccaatcg
     5761 cgacatcctg catttctgta gatgcaatta taccaggctt tgagtctttt gcccgcggaa
     5821 tgatttcaat atatcgctta acaatagtct tgtctgtttc actctcagtg attgttgtat
     5881 tttgttcttc cgcacctgtt acctctctat ctttttttcc atgaaggata ctttcctggg
     5941 tttgttccgg agctttggat aaaatagatt cttcaaaaga ccgtggctgg ttaaaataat
     6001 tgaaaagcaa aacgcccgaa gcaagcgcaa gtgacaccgc cgccaccatt tgccaaatta
     6061 ttaagtttcg attatccttt ttggaatctt tcttattgag ctcaaagtca attctttccc
     6121 aaacaccgtg agatggcatt gcctctgcct gggaaaaagc tgacctccat tcattttcaa
     6181 attgatattt atgctctttc atacctaacc atttcatttt tagaaatcaa ttcttttaaa
     6241 agtatttttg ctcttgaata ttgtgacttg gatgtacctt cacttatgct cagcatttct
     6301 gcaatctcgt tgtgattata tccttcaatg gcaaagaggt taaatattgc ctggcagcca
     6361 gatggtaatg attgaatcat ttttagcagg tcttgccatt cgaaacgggc gagagaaatc
     6421 tcgtcttcca gatttatctc attaacatct gccatgggga acatatataa tttacttctt
     6481 tgactgttaa gggcggtatt tattacaatt cttgtcagcc aagtttccag cttcgatttt
     6541 cttttaaact cccggatttt atcaaatact ttaataaatg aatcctgaat aacgtcttca
     6601 gcttctgact gggacttcgt atatcgcaag gcaataacat aaagctttcc cgaatatcga
     6661 ttatacagat attcctgggc cttacggtca tttcggcaac aaccctctat aagttcttct
     6721 tcattcatcc ggtagctact cggagtttat tgttagaccc cggttattta gacttggttg
     6781 gaatatttcc ctcaaatttc cgttgttaat tgtaatcaag aaaaaatgaa gccaaattta
     6841 gttttatgaa agcgaactta aatattactt tggtatttct cttattatcc tgtgttccag
     6901 tgccttatag ctcaacccaa tcaggtggaa aagaccttct tttcagagat ttcaattatg
     6961 aagcttcaat aaaatcagtc ttgctctatc cggatgataa tatcggcgtc cccaaccttc
     7021 agcctggcac tttgtcactt ttatcccaat caagcctggt tcttgaattt gattacctga
     7081 gcgaaacacc ggaaagcttt tatgccagga tttatcattg cacgaaagac tggaagaagt
     7141 caaacctttc agacctggaa ttcttacaag tatataatga atttaattta aacgattacg
     7201 cgtactccat tgacacgaaa gtggattaca ttcactacag gtttcaactt cctaaagtga
     7261 aactaccggg aaattacgta ttgattattt atagaaataa tcctgacgat ttcattctct
     7321 cacgaagatt tcttgttttt aatagcagaa ttcaaatcag ccttgccggc aatctaaata
     7381 ctataaatcc tgtgttgatg agtgaccact tgatcgaatt cactctgaat tatcaatcaa
     7441 tcccactgga tgatcctatg ggaaatatca gtgtggacat taggcaaaac caacaatggg
     7501 aaaattatat tcatgacctg aaggccaatt tcgtcaggga agatttaaag gagttggagt
     7561 tccgatactt taacctcgaa aatgctttta aagcttctaa cgagtttcgt ttttttgacc
     7621 tgcgatcagt tttaaatttt ggtcagaatg ttgaaaatgt aattaatacc ggagaaggat
     7681 ttacggcaat attggaaaaa gaaaagccaa ggtcagtaca ggcttatggt cacctgaatg
     7741 atctcaatgg taaatatact cttgaaaatt atgatcggcg aaacccgctc accggctcag
     7801 agtatgttaa catattattt actcttgagt ctgaaaaaat tatcggagat gtgtacataa
     7861 cgggggagtt aaccaattgg ggtcaaacca gccctatggc gtacaacgag gaactgaagg
     7921 catacactga taatttatta ttgaagcaag gatggtataa ttatttattt aaagttgatt
     7981 cacccactct gccctcatat tacctggaag gctcacatgc ccaaactgag aatgaatatg
     8041 aaatattagt atactaccgg gatataatga aaagggcaga cctgcttcta gggtataaac
     8101 gattcataat taatagccgc taaacattaa aacgaaaatg catgatatcc ccatctttga
     8161 ccacatattc tttcccttca atggctaact ttccagcttc cctgcaagat gcttctgttt
     8221 tatatttttg gtaatcctcc aattttatta cctccgcttt aataaaaccc ttttcaaaat
     8281 ctgaatggat tactccggcc gctttcggtg ctttccatcc tttctttatt gtccatgccc
     8341 taacctcctt aggaccggca gtaaagtaag aaatcagatc gagtaattta tatgtggctt
     8401 tgataagtct gtccaatccg gattccttca atccatactc ctgcaaaaac agcaaacgct
     8461 cttcaccatc ttccaattca gcaatttggg cttcaatggc ggccgatatc aaaacaatct
     8521 cagcttcttc accggttatg gcttggatta atttctcaac ataagaattt cctttcagaa
     8581 cagaggcctc gtctacattg gcaacataaa gtacaggctt ggatgttaac aattgcaaac
     8641 cctttacaaa ttcacgttca ttaatatccc aggcgatggt acgggcactt ttaccctgtt
     8701 caaaatgaat ttttagtctt ttgaaaatat ccagttcctt taatgcggat ttgtcccctg
     8761 atttcgcgat cttttcaacc tttgaaattc gcttatcaat tgtttccaaa tctttgagct
     8821 gaagttcggt atcaataatt tccttgtcgc ggatcgggtc aatacttcca tcaacatgca
     8881 cgatattttc attttcaaaa catctgacca catgaattat ggcatcaact tccctgatat
     8941 ttgccagaaa ttggttgccc aatccctccc ctttgcttgc accttttacc aggccggcga
     9001 tgtctacaaa ctcgatggtt gttggaataa ctttctcagg atgaacgagt tccttcaaaa
     9061 tatccagtcg ttcatctttc actgaaacaa ttccgacatt gggttctata gtgcaaaatg
     9121 gaaaatttgc cgcttcagct ttggcacttg aaacagcatt aaacaaagtt gacttgccta
     9181 cattaggcaa gcccacaatt cctgatcgta aagccatgta ataaattaat ttctaaaaac
     9241 cttgtactgc ctgtagccgg aataaagttc ctgaccggag acccggagaa tagtgtagaa
     9301 gggtattgcg gaaatcattc caagtactcc tcctaataac cctccgacta ttaaccttcc
     9361 cagcgcggca ccggcaaaga taactacaaa aatttctaat ggatgagctc tcaggctttt
     9421 tgaaaaaatt aaaggttgga aaatcaggtt gtccattatt tggacgactg caaaaacaac
     9481 cgcgatctta acaacaagaa aaatattctc ctgcggtgtc agatttccca gttgtgttga
     9541 aatggccaca aaaattccaa agagacttcc caggagcgga cccagataag ggatcaaatt
     9601 ggccaccgcg gcgaaaaatg caattgtcaa agcataattt acaccaacca acgtaagacc
     9661 aattgatgcc actgagaaaa tcgcgaacat ttgaaccagc aatcctaaca aataattaga
     9721 gaacaatttt tctatcttat taaaggcgga gattgtaact tcaaaataag cattaggtat
     9781 taacgacagc attctctttc tgatagtggt cgtttcataa agcaaaaaga aagtgatgaa
     9841 taagatagcc atccctccaa tgaacaaatt gcctgtaagg gatagaaacg tattcaaaat
     9901 attctgaatc ttgagttctt taaccagatt aaccatcgaa tttctcagtt cttccaggat
     9961 aaatccattc gcgttttcgg tgagattgta atcgataaga aagttttcaa ttctggtaag
    10021 tggctccgag attttattga agatgttttc ataatccaaa gaagaaagga catcaatttg
    10081 ggctgaaacc actggaataa aaagcgtaaa aaataatgag ataacacccg aaaaaattac
    10141 aaaggaaaga aaaactgcga atgccctggg aagacggatt tttagaaatt gagttttgct
    10201 taatttttca accacgggct ttaatagcgc cgctatcaca atgctgatta tgaaataggc
    10261 aagaatgtct gtgaatatcc aagccaataa cacaaaaatc aggatactca gtattaaata
    10321 aataattgtc cgatttacct gcattgattt aatagacaat aaaaaagccc ttgtatgcat
    10381 tcaagagctt caaaatttct ttgaaaagtc ttctactccc acttcaattc gctatcgatg
    10441 cctgattctg ccttttcttt ttctttttcg ggctcttctt ctctatcaaa ttgtgcgaaa
    10501 tcaacttcag gcaaaagctc aactttaaca tggtttactg tgctgttcaa agcagcaata
    10561 aacttgttaa agtcttcctt gtaaagaaat attttgtgct tttcgtaggt gtagccatca
    10621 tctttatacc gccgcttact ttcggtaatg gtaagataat aatcatttga cctggtggat
    10681 tttacatcaa aaaagtaagt tcttttacca gccctcactc tttcggaaaa aatttcctgc
    10741 ctgtcatttc cgttttgctc ttccactttc ctttagtttt gttgagtaaa attttggtta
    10801 aatatcattc aaattacagc taaatatagc ataataacca taagtaaagc aaatcaatac
    10861 ccaacatata tggccattgg cacaattttt acccggtcaa ggattaaatt ggaaactcat
    10921 attttagctg ttttaagccc tttttgacca tgaagataga tttaaatgaa attcgcaact
    10981 ttgcaaacct ggaattgctt gcaaaacaac ttgttgaggg attcatcaca ggtctgcata
    11041 agtctcccta tcacggattc tcagtggaat ttgcagaaca ccgtctctat aataccgggg
    11101 agagcactcg ccatattgat tggaaggttt tcgccaagac cgatcggctt tatgtgaaac
    11161 gatatgagga ggaaactaat ttgaggtgtc agattgtaat tgacaattcc tcctccatgc
    11221 attatccggt tgccaacagg ggaaaattgc tattcagtgt gcttgctgcc gcttccctgg
    11281 cttacctttt acaaaagcag cgtgatgccg taggtataac taccttctcc gataaaattg
    11341 atttgcaaac acagattaaa tcaacttctg ctcacctgaa taaacttttc attgagctgg
    11401 agcatcttct cactgaaaat atcaaactga aaactaccaa tgtggcgccg gtgctgcacg
    11461 aaattgcgga caaaaatcac aagagatcat tagtaattat tctgagtgat atgtttgaca
    11521 gggatactga acttgatgaa atttttaagg cccttcagca tcttaaacac aataatcatg
    11581 aagtattgct atttcatgtg acagatcagc aaacagaata ccgttttgaa ttcgaagacc
    11641 ggccctatga attcattgat ttggaaagtg gagaaaagtt aaagataaac cctacccaag
    11701 taaaagacat gtataccagg aaagtaggcg aatattataa tgagctaaaa cttcgctgcg
    11761 gacaattaaa aattgacttt attgaagctg atatcaggga cggattcaat aatgttctta
    11821 aatcctacct gattaaaagg tcaaggatga aataatctaa tgaccatgca tccaggcttt
    11881 cttatttaac aactcttcct cagtttcctc cttggctaaa tcaggtaagc aacaatctac
    11941 gggacaaact gccgcgcatt gtggttcatc gtgaaaaccc ttgcactctg tgcatttctc
    12001 cggaacgata taataaaatt cgtccgacac cggaagcctg ccggattcgg catccacagt
    12061 agatccgttc attccctgaa tcgttccttt caaggttgtg ccagtggcaa aattccactc
    12121 tatgccagct tcataaatcg cgttgttcgg gcactccggt tcgcaggcac cacaatttat
    12181 gcattcttct gttatcttta ttgccataac tattttgggt agtatctcag tttggaatca
    12241 ttatacgtca ttatacgtgt gccacacttt ttcgacacgg aaaagcaatc aatttcacct
    12301 aataaagact gaagtgtggc actcgttaga tattaatttc attgtgatct taactgagac
    12361 actactctat tttgcggtaa cggtgcaaat ttattgcact taacagtcaa tacctgatgg
    12421 acaaggaagt atttgaaaca tttattattc tgggagaaaa aataagcaag aacattcttc
    12481 gaataccttt caagaataca caaaagaata atccatggtt tgatgaaact tctgtttcgg
    12541 ccgctcttca cggaatttcc atttaccttg aaaaagataa tatgaccaat tggttgaaag
    12601 attattcctt taaaaagagc cgaaaaacta ttggcctgat tgctgccgga aatattcctc
    12661 ttgttggatt tcatgatctg ctttgcatcc tgatctccgg ttttaaggtt gaaataaaac
    12721 caagttcaaa ggatttacct ctgacaaaat ggatcattcg gcaaataacc aacattaacc
    12781 ccgcgcttca gacgagaatt tccttaaagg aaaaacttgg taactgcgat gcctacatcg
    12841 ccacgggaag caacaatact aaccggtatt ttgaatatga attcagaagc aaaccacatc
    12901 ttctgcgaaa taacagaaca ggaatagcca tattgacagg taaagaaact cagcgagatt
    12961 tgaatggcct tgcagccgat atatttcaat attatggctt gggctgcaga aatgtatccc
    13021 tgctccttct tccaatgggc tacaaacttg ttaaaatcag caagtgcttt ggtcagcacc
    13081 aaaaggttat ggataacaaa aattatagcg acatttataa ttacaacaaa gccttgttga
    13141 tacttggcaa aaaaaaattc catgatttgg gtaatttgct gcttgtccca aataataatc
    13201 ccaacgcaac cgtttcagtt ctccactacg atttttacaa aaatcccaat catttaaact
    13261 cattcatcag aagaaaccgt gaacttcttc agtgtattgc cgggcataga atatccggaa
    13321 tttcaaccat accttttggc acaacacaga ccccggaggc atgggattat aacgatggga
    13381 agaatactct ggaatttctt tgcaatttgt gaggtttccg ttacaataaa ttaaggaaac
    13441 cgttttattt ctgatatttc tttgtaatta attaccttta caaaccttga ctgatcaagg
    13501 atttaaccac aaaattatgg catttgatat tgatatgata aaattggttt acaccacatt
    13561 cggggagcga gttcatgccg cccggaattt actggggaag cctctcacac tttcagaaaa
    13621 aatattatac gctcatctgt ttgacggaac ccctacccag gtttacgaaa gaggaaagag
    13681 ctacgtcaac tttgcaccgg atagagttgc catgcaggat gccacggctc agatggcttt
    13741 gctccagttt atgcaggcag gaaaaaaaaa tgtggcggta ccctcaactg ttcattgcga
    13801 tcatttaatt cttgcaaaag acggcgccaa ggcggatcta aaaaattcaa tcacggcgag
    13861 taaggaagtg ttcgatttcc ttgaatctgt atctaagaaa tatggaattg gcttctggag
    13921 accaggtgcg ggaattattc accaggtcgt actggaaaac tatgccttcc ctggcgccat
    13981 gatgataggt actgattctc acaccgtaaa tgccggtggg ttggggatga ttgccattgg
    14041 agttggcgga gcggatgctg ttgacgtaat ggccggtatg gcatgggaat tgaaaatgcc
    14101 caggttaatt ggcgttaaac ttacaggcaa gctcagtggt tggactgcat ccaaggacgt
    14161 tattttgaaa gtagccggaa tcctaacggt gaaaggaggg acaggtgcga tagtagaata
    14221 ttttggtgag ggagctcaat cactttcttg tactggcaaa ggcacaatat gtaatatggg
    14281 agctgaaatc ggggcaacta catcaacctt tggctatgat gatatgatgg aaacctacct
    14341 gcgtggaacg ggcagaaacg acgtagctga tctggctaat aaaatcagag cacatcttac
    14401 aggagatgat gaggtctatg caaaccctga caagtatttc gaccaggtta ttgaaataaa
    14461 tttaagcaaa cttgaaccac atataaatgg tccctacact cctgaccggg caactcccat
    14521 atctcaattt gcagaggaag taaagaagaa tggctggcca caaaaattag aagtcggctt
    14581 gattggttct tgtaccaatt cttcctatga ggatatctca cgcgctgctt caatagcaga
    14641 gcaggcatta gccaagaatc tcaaggtaaa atctgaatat accgtaactc ctggaagcga
    14701 gcaggttcgt tacacggtag aaagagacgg atttcttcaa aaattcgaac aaattggcgg
    14761 cgtaatcctt gctaatgctt gcgggccttg cattggtcag tggtcccggc atacaacaga
    14821 ccccgatcgc aaaaatagta taattacttc cttcaaccgg aatttctcca aacggaatga
    14881 tggaaatccc aatacgcatt cgtttgtagc ctcacccgaa attgtgacgg ctatggcaat
    14941 tgccggtgat ctcaccttca atccgctaac ggataaactc aaaaatgaaa atggggagga
    15001 tgtcatgctc gatgaacctc atgggattca aatgcctcct accggatttg cggttgaaga
    15061 tgcaggatat caggcacctg cggaagatgg atcgggaata gaggtaattg taaagcctga
    15121 ttccaaacgc cttcaacttc ttgcaccctt tgagccatgg gatggaaata atattaccgg
    15181 aattaggttg ttgctaaagg caaaaggtaa atgtactact gatcatatta gtatggctgg
    15241 accctggttg agattccgcg gacatcttga taatattagt gataattcat tcactggagc
    15301 tgtaaattat ttcaatgaca aggctgattc ggtgaagaat ttactaaccg gcgagtatgg
    15361 ccctatcccg gccacccagc gggcatataa agcaaagggt attcctacta tggtagtcgg
    15421 tgatcataat tatggtgaag gctcttcaag ggagcatgcc gccatgcagc ccaggcacct
    15481 gggtgtgaag gcaatcctgg taaaatcatt tgccagaatt catgaaacaa atctcaagaa
    15541 acaaggaatg ctggctttaa cttttgccaa cgaaaatgat tacgataaga ttctggaaga
    15601 cgacataatt gattttaaag atcttcctga ttttactcct gatgcaccac ttacattagt
    15661 gcttaaacat tcggatggaa accaggagga aataaaagta aatcactctt acaataattt
    15721 tcagatagaa tggtttaagg cggggtcagc tcttaacctt attaagaaaa aggaagcggg
    15781 aatttaattc tcgctttttt atttcaatgt aattccggat taattaatga tgaaaagaat
    15841 tctttggatt atactcattg caccgccagt gctcgcattt atggcaaagc cttccacatt
    15901 tgaaatcaag gtgcttggca cttatactga tgccattgcc ggaaactcta aagagcgaat
    15961 agatcttaaa tcacttgatc tgaaaaggga aagttttgga attggtcttg atgctgattc
    16021 cagggctgaa atcctgattt ataaagggaa gattttcaaa tcatcattca ataataaagg
    16081 tataattact gagaaaacta ataattctac cgcctcctat attgtttata catcagtcag
    16141 aaaatgggaa agctatttgc ttccaagcac aatcagatcc cttgccgatc tggagactga
    16201 aatagcaaag gtgattgaaa ataataatta caccggttcc ggtacaattc catttttatt
    16261 gataggaaat cctatctctg caaattggca cattgaagta acaccaggta ccaacaagca
    16321 gctaaataac cggggattct tttttgaaga taagattaag gccgtgggtt tctttaccac
    16381 agaccaacaa ggtgtgatta ccgaaatgaa ctctcgaata catgttcatg taaccaataa
    16441 acgtaaagaa atcgtgggac atctggatga tatcaaaatg gcaggcggga tgactttatg
    16501 gatgcccaaa cttaaatgaa gctgctgtaa gcaatgttaa tacactgcca aacctgttaa
    16561 tagaccctct ggttttatta tatttgtaaa aaaattgtta tggcgaagaa aaagaatacc
    16621 gcgaaatcaa aacaagtaat tacagctaaa agatcaatta ccaaagaaac ggaaaatctt
    16681 ttaaataccc agataattat ggaggggaca tcctcagctt attacttgtc tatggcttcg
    16741 tggtgccata caaaaggata tgattattca tcaagatttt tatacagaca ttcagaagaa
    16801 gaaagaatgc atatgctgaa acttttcaag tatataaatg atgcaggcgg acacgctctt
    16861 caacctgaaa ttacgaatat tcatcatacc tttaaatcac tgcgagaagt ctttaattta
    16921 attctggggc atgaaataga ggttacaaaa tccatcaaca aaatagtgga tcattgtttc
    16981 tcaattaaag actttacaac tttcaatttc ttgcaatggt atgttacaga acaaagggaa
    17041 gaagaaactt tggctagacg agcagtcgag ttgtttgatt tgattggcga agcaggcatc
    17101 gggttgtgga caattgatca ggagattgga aagcttgatt cttttgcagc caactcggcc
    17161 ccggaaaatt agaatctacc gcattagttc ctttaattca ctaagtatca tggccgtagc
    17221 accccacaag acctccccct ccaggtcaaa atagggagtt tctaatttta tcacttcatt
    17281 tgcttttcct gatttacgga taatttctat ctccttgtta aggacgttat tgccttccac
    17341 aaagtaactc accggggcct caattatttt ttgtacctca accggatcaa ttttaaactg
    17401 aggtttcgac tcgacaaatc caacaaaagg attaaccatg aaattacttg gaggaatata
    17461 aagcggagta agactgccaa gtatttttat ctggttaatt tgcgcgccaa tctcctccca
    17521 ggtttccctt aatgccgtgt ctaccagatt cttatcttgc acttcacgct tcccccctgg
    17581 aagactgatt tgtccactgt ggataccgtc ataatcgggc ctttttatca atggtatata
    17641 agcagagcca ttttggggaa aaatcagcac cagtacgctg ctttctttat agcctttaat
    17701 ctccgaataa ttgaatcgcg aattttcagt tcg
//
LOCUS       DASWHX010000132        10082 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_3091869, whole genome shotgun sequence.
ACCESSION   DASWHX010000132 DASWHX010000000
VERSION     DASWHX010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 10082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10082
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3091869"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(165..947)
                     /locus_tag="VGA21_09090"
     CDS             complement(165..947)
                     /locus_tag="VGA21_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010608108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX9650704.1"
                     /translation="MKNYNNLLLETKEGILTLIISRPSSLNALNLETMKELDEVFDEI
                     LTDEQVKGVILTGAGEKSFVAGADITELANLDAKSAKSFVQNGHRILAKIENCPKPVI
                     AAINGYALGGGCEIAMACHMRTATNTAIFGQPEVNLGVIPGYGGTQRLTRLIGKGKAI
                     EFMTTGDSIKADEALSLGLVNHIFNSQSELMDGTIQLMRKILSKAGVAISMVLDSVNS
                     YYDSRNGFENEAANFSRSVDTMDFKEGTRSFLEKRKPQFKGK"
     gene            complement(952..1896)
                     /locus_tag="VGA21_09095"
     CDS             complement(952..1896)
                     /locus_tag="VGA21_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014770851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX9650705.1"
                     /translation="MEKILVIGALGQLGSELTASLAEKHGLDNVIATDIKPFSAKTFS
                     CKYFQLDALNKEAILKSVQENAISQIYHLAAILSAKGEQNPSKAWDINMTSLLNVLEV
                     GKEMNSKIYWPSSIAVFGSGTPKENTPQNTVMDPNTVYGISKLAGERWCEYYFQKYAV
                     DVRSLRYPGLIGWRALPGGGTTDYAVEIYHHALQSGEYTCFLEKNTRLPMMYMNDAVR
                     ATIELMDAPASNIRIRSSYNVAAMSFSPAEIAKSIAHKIPGFRIKYSPDFRQKIAESW
                     PASIDDMKAREDWNWQHEFDLEKMTAQMLANLEQTINK"
     gene            1998..3197
                     /gene="kbl"
                     /locus_tag="VGA21_09100"
     CDS             1998..3197
                     /gene="kbl"
                     /locus_tag="VGA21_09100"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009581919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HEX9650706.1"
                     /translation="MYTHLKPVLRKELDSIKEAGLYKSERIITTPQGAVIKTTSGGEV
                     INFCANNYLGLSSHPMVIEAAKSGIDTHGYGMSSVRFICGTQDIHKELESRISKFLGM
                     EDTILYAAAFDANGGVFEPLFGPDDSIISDELNHASIIDGVRLCKARRFRYKHNDMED
                     LRKQLDAASGTKQKIIVTDGVFSMDGTIAQLDKICDLADQYGAMVMSDECHSTGFIGR
                     TGRGVHEFRNVMDRIDIITGTLGKALGGASGGFTSSKKEVVEMLRQRSRPYLFSNTLA
                     PSIVAGTIAVIDMLTETTELRDKLEANTLYFREKITEAGFDIKAGIHPITPIMLYDAK
                     IAQKMAEKLLEEGIYVIGFYYPVVPEGQARIRVQISAVHENEHLDKAIEAFTKVGKEL
                     GVLKNQS"
     gene            complement(3194..4072)
                     /gene="cdaA"
                     /locus_tag="VGA21_09105"
     CDS             complement(3194..4072)
                     /gene="cdaA"
                     /locus_tag="VGA21_09105"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017732033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HEX9650707.1"
                     /translation="MMLGFRIGFLEITWVDFFDIALVSILLYQLYKLLKGSVAVRIFF
                     GFLFLYLVFLVVNAAQMELLSAILGQFMGVGVLAVIILFQQEIRKFLLMIGKSTSFDR
                     ENLLNNFRIWKRSEQQRDYHISVIIEAAKTMGVSYTGALIVFSKSSELKFYAESGDIL
                     DAQISKRLLLSIFNKNSPLHDGAVIIYKGRIKAARCILPVSESDTLPANYGLRHRAAM
                     GMSETTDTLVLIVSEETGHISIARGGSIYADLSPVEVRRKINEYLFEEKESEKTAEET
                     EVQTASETGELKEIKS"
     gene            complement(4069..4911)
                     /gene="folP"
                     /locus_tag="VGA21_09110"
     CDS             complement(4069..4911)
                     /gene="folP"
                     /locus_tag="VGA21_09110"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007652300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEX9650708.1"
                     /translation="MEQDTLFSKKTLTLGGDVIDLTTPICMGILNITPDSFYEKSRKS
                     GDAIIAQAGQYLEEGASILDVGGYSSRPGAEHISLEEELQRVIPAVRNIISHFPGTHI
                     SIDTFRAEVASAAIEEGADMVNDITGGEGDPDMFNLVASKNVGYVLMHMRGTPQTMKN
                     LTDYSNLLEEITEYFRSRISVLDKLNVNKIVIDPGIGFAKTADQSFEIIKNLRIFDIL
                     KKPVLVGVSRKSLIQKILGNTSGDALNGTTVLNTVALLNGASILRVHDVKYAMEAIKL
                     VKEL"
     gene            4903..5490
                     /locus_tag="VGA21_09115"
     CDS             4903..5490
                     /locus_tag="VGA21_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009193690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1599 domain-containing protein"
                     /protein_id="HEX9650709.1"
                     /translation="MLHYKNIDQKDVSDTVREYNEVIKGCKELFKKKTRDYGTAWRIL
                     RLPSLTDQIFIKAQRIRSIQEKGVSKIAEGIQEELIGIINYCVMSIMQMEFEEKDKVE
                     MTYEELSPIYDRITKETMELFMDKNHDYAEAWREMRVPSITDIILMKVLRVKQIEDNE
                     GTTLVSEGVKSNYQDIVNYSVFALIKLNYENNENS"
     gene            5477..6574
                     /locus_tag="VGA21_09120"
     CDS             5477..6574
                     /locus_tag="VGA21_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007652302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BT_3928 family protein"
                     /protein_id="HEX9650710.1"
                     /translation="MKIVDWIAKIIVGGLFIFSGLIKINDPVGTQIKLEEYFEVFATD
                     FGSFFHLFMPIALPIAFILIILEVVLGVAVLINFRMKISSWVLLILIFFFTFLTFYSA
                     YFNKVTDCGCFGDAIPLTPWESFYKDLILVVLIAYIFVRRKLLDALTGEKTGNFTISG
                     TALATLIIGIYAVRHLPFIDFRPYKIGDNLMVNMQPREEPIFEYTFRKDGELIKSEQY
                     LSEQEGYEYIGYEVKNSKNTIPKITDFSVWNDEVGEYTDLALTGNKFILIVQDAGKAS
                     RKDLAKIGNLMKTVTLQAEVLVFTSSGVASLQDYLVEARVAVPVFFVDATVLKTMIRS
                     NPGMMLMKEGTVLGKWHYNDIPVPDQVLNLL"
     gene            6574..7074
                     /locus_tag="VGA21_09125"
     CDS             6574..7074
                     /locus_tag="VGA21_09125"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF013375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEX9650711.1"
                     /translation="MKIYLLGMPGSGKSTIGKMLASYLGLSFIDMDMEIETETGKSIK
                     EIFEKKGEKHFRFLEKQLLQMLAGKDSIVVATGGGVPIHNNNMKLINETGISVYLKAS
                     IEQLQSNLEESYSARPLLQKKNLKDSLMQLLTEREEVYSKSRYIIQVSEDPKSTVREI
                     VEIFKN"
     gene            complement(7064..8566)
                     /locus_tag="VGA21_09130"
     CDS             complement(7064..8566)
                     /locus_tag="VGA21_09130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650712.1"
                     /translation="MRKASLLILISVGGLWPGRHFAQGLTGEVLLFSQSPVSGSARIQ
                     SLGFSQVALGGDISSAFSNPAGLGFFNRSEASFTPALNFNSADSYLELSGNSRSQSVS
                     DSRAVLNFANLGVVFHRPIENNEFLKGGSFGLSISKTKDFNNRITYQRDKDNVDVDYF
                     DFVFDKFDNGNEDTFSDLAFEAYLVDLFEIDDGSGNLIQYYDYVASDIETLDYPDLQR
                     ETRISKGSEYSVNIAYGANFGDKVYLGATIGLLTLDYHENSRYSEFRSNGSLSEFTLE
                     EDLNITGAGINATFGIMVRPVDMLILGASLSTPNFYVFEDLYEAKLTSTFDNYFYIPE
                     NKQLTTEEALSDIFVSQYNLRTPLKFNTGASVFIGKHGFITGEVEFLNYSYNHISSDD
                     FETVDDNQLIDDQFGFVTNYKLGAEFRRGIFRLRGGFAFFDDPRKSSSVDSSRSNITF
                     GGGIRLPKFYVDLGIITSKYDSSIRPFASSVYNTVENKRVSALITAGFNF"
     gene            complement(8578..9906)
                     /locus_tag="VGA21_09135"
     CDS             complement(8578..9906)
                     /locus_tag="VGA21_09135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650713.1"
                     /translation="MKRITGLTVAALSALILGACSSSQLAHQGEVDDMYYGSADRQRE
                     SYKDVNFNAYSSFREDANPLKLDGEEDLTVDNYSAKTLNPDYISKYNSELSEDEGSEY
                     GEGDYYAEGYDSSEDEKSTIINNYYGSGYGIPYRYNYRYYDPFYWDPFYSGYYYGSGI
                     RVTISYSYGWGYNRWNNWYLYDSYYGYSPSWVYAAMYCPPSGFYYGSYYGGGYYSSYG
                     YSNSLYYNNSYVNINPDNINGNTRKIVKGPRPSRGVVSSLTENNNSRSTVSNITNPSR
                     SRTQTITDAPSQSSYYRRSRQDNQIISRDEIDRNSASRTTPQIINQRSTQSTRTSTNY
                     VRPKTNRSSLYDVRTNQRSSGYQVNPNTNNNTRQRSSQDFNRQNSNQQWNRTAPGSNR
                     DYQINRSSTPSRSTAPARSNVAPARSSTEKSSGTRSGNNNSSSSRPARKN"
BASE COUNT         2975 a         2005 c         2046 g         3056 t
ORIGIN      
        1 cataagaata aagtaatgtt atggatccaa attctgaagg aatgaaaact gctgtatgaa
       61 gtggtacaag gaaataattt aacaagcggc caaatatgct gcttactccg tatagtgcag
      121 tttgaccggc aagttgcttg agggatccca ttcagggttc ggggctattt gcctttaaat
      181 tgaggtttcc ttttttcaag gaaagatctg gttccttctt taaaatccat ggtgtcaaca
      241 ctgcgggaaa aattcgcagc ttcattttca aatccattgc gggaatcata ataggaattt
      301 accgaatcaa gtaccataga aatagcgaca ccggcttttg agagtatttt ccgcataagt
      361 tgaattgtgc catccattaa ttcagactga gaattgaata tgtgattaac taaccccaga
      421 ctcaaggctt catcagcttt gattgaatca ccggttgtca taaattctat tgctttgcct
      481 ttgccgatca aacgcgttaa acgctgggta cctccataac ctggaattac tccgagattc
      541 acttcaggct gaccgaatat tgctgtattt gtcgctgttc gcatgtggca ggccatagca
      601 atttcgcacc caccgcccag ggcatatccg tttattgccg caattacggg tttgggacaa
      661 ttctcaattt ttgcaagaat tcgatggcca ttttgaacaa atgactttgc agatttagcg
      721 tcaaggtttg ccaattctgt tatgtcggct cctgccacaa acgatttttc tccagcaccg
      781 gttagaatta ctcctttcac ctgttcatct gttaaaattt catcaaaaac ttcatccagt
      841 tctttcatgg tttccagatt cagtgcattg agactggaag gacgtgagat aataagtgta
      901 agaattcctt cttttgtttc gagcagaaga ttattataat ttttcattga ttcatttgtt
      961 aatcgtttgt tccaggttag ccaacatttg tgctgtcatt ttttcaaggt caaattcgtg
     1021 ctgccagttc cagtcttccc ttgccttcat atcatcaata gaagcgggcc atgattccgc
     1081 tattttttgt ctgaagtcgg gagaatattt aattcggaag cctggaattt tatgcgcaat
     1141 ggatttagca atctccgcag gagagaagct cattgctgca acattgtagc tggagcgaat
     1201 tctgatatta cttgcgggtg catccattaa ttcaatagtt gctctcacag catcattcat
     1261 gtacatcatg gggagtcggg tatttttctc cagaaagcag gtgtattctc cactttgcaa
     1321 agcatgatgg taaatctcaa ctgcataatc agtggttccg ccgccaggca atgctctcca
     1381 gccaatcaaa cctggatacc taaggctcct gacatccact gcatactttt gaaaataata
     1441 ttcgcaccat ctttcgccag ccagtttact gataccataa acggtattgg gatccattac
     1501 tgtattttga ggtgtatttt ccttcggggt accagaaccg aaaactgcga tgctgcttgg
     1561 ccagtaaatt tttgaattca tttctttgcc aacttccaga acatttaaca agcttgtcat
     1621 atttatgtcc caggctttag acggattttg ttctcctttt gctgataata ttgcggcaag
     1681 atgatagatt tggctgattg cattttcctg aactgattta agaattgctt ctttattcaa
     1741 tgcatcaagt tgaaaatatt tgcatgaaaa ggtttttgca gaaaatggct taatgtcagt
     1801 agcaataaca ttatctagtc catgcttttc ggctagtgag gcagtaagct cagaacccaa
     1861 ctggccaagg gcaccaatta ccagtatctt ttccatgcaa attgtttggt tccaaaatac
     1921 aaatattatg aatccttaaa aacctgaacg gttattaaaa aggtttttat ctttgttcaa
     1981 acgaaaaatt tgatctcatg tacacccatt taaaacctgt tctaagaaaa gaacttgatt
     2041 ctattaagga agcaggtctt tataaatcag aaagaattat aaccactccc cagggtgcag
     2101 taattaagac tacctccggg ggtgaagtga ttaatttctg tgcaaataat tacctgggac
     2161 tctcaagtca cccaatggtg attgaagcag cgaaatcagg aattgatact catggctatg
     2221 ggatgtcatc cgtgcgattt atttgtggta cgcaggatat tcataaagaa ctggaatcca
     2281 ggatttctaa attcctggga atggaagata ctatattgta tgccgcggcc tttgatgcaa
     2341 acggaggtgt attcgaacct ttatttggcc ctgatgattc cataatctca gatgagctga
     2401 atcatgcttc cattatagac ggtgtaaggt tgtgcaaagc ccggcgtttc cggtacaagc
     2461 ataatgatat ggaagattta agaaagcagt tggatgcggc atccggcaca aaacaaaaaa
     2521 ttattgtaac agacggagtc ttctccatgg atggaacgat tgctcaattg gataaaatct
     2581 gtgatttagc tgatcaatat ggcgcgatgg ttatgtctga tgagtgccat tcaacaggat
     2641 ttataggacg gactggacgg ggggtgcatg aatttcgtaa tgtgatggat agaatcgata
     2701 ttatcacggg aacccttggc aaagcattgg ggggcgcttc aggtgggttc acctcttcaa
     2761 aaaaagaagt cgtagaaatg ctacgccagc gatcccggcc atatttgttt tccaatactc
     2821 ttgcaccttc catcgttgca ggaaccatag ctgttattga catgcttaca gaaacaacgg
     2881 aactccggga taagcttgaa gcgaacacac tttattttcg tgaaaaaatt actgaagcag
     2941 gttttgatat taaagccggt atccatccta taactcccat aatgttgtat gatgcaaaga
     3001 tagcccagaa gatggccgag aaattattgg aggagggaat atacgtaatt gggttttatt
     3061 atcctgttgt tccggaaggc caggcaagaa tccgggttca aatttcagct gtgcatgaaa
     3121 atgagcatct tgacaaggcg attgaggcgt ttactaaagt aggaaaagaa ttaggggttc
     3181 taaaaaacca atcttaagat tttatttcct ttagctcacc ggtttccgaa gcggtttgca
     3241 cctcagtttc ttctgcggtc ttttctgatt ccttttcctc aaacagatat tcattaatct
     3301 ttcttctaac ctcaaccgga gaaaggtccg cgtaaatact tccaccgcga gcaatactaa
     3361 tgtgaccggt ttcctcagaa actatcaaca ccagggtatc tgtggtttca ctcatcccca
     3421 ttgctgcacg gtgccgtaaa ccataattgg caggaagagt atcgctttct gaaaccggaa
     3481 gaatgcagcg cgcagcctta attcttcctt tgtaaataat tactgctcca tcgtgcaacg
     3541 gactattctt gttgaagatg gaaagcagta accgttttga aatttgagca tctaggatat
     3601 ctccggattc agcataaaat tttaactcgg agcttttgga gaaaacaatc aatgctccgg
     3661 tgtaagatac tcccatcgtt tttgctgctt caattatcac cgaaatatgg taatcgcgtt
     3721 gttgctcact tcttttccaa attctaaaat tgtttaacag gttctcccgg tcaaaagacg
     3781 tacttttacc tatcattaaa agaaattttc tgatctcctg ttgaaaaaga atgattaccg
     3841 ctaatacacc aacacccata aactgtccaa ggatagcgga tagtaactcc atttgagcgg
     3901 cattcaccac caggaaaacc aggtacagga agaggaatcc aaagaatatc ctgaccgcca
     3961 cgctgccctt caacaattta taaagttgat acaacaggat gcttaccagg gcgatatcaa
     4021 aaaaatctac ccaggttatt tcaagaaatc ctatcctaaa tcccaacatc aaagctcttt
     4081 aactaattta attgcttcca tagcatattt tacatcgtga actctcaata tggatgctcc
     4141 attcagaagt gcaactgtat tcaatacggt agttccgttc aatgcatcac cggaagtatt
     4201 tcctaaaatt ttttgtatca gcgacttccg ggacactcct acaagaacag gcttcttcaa
     4261 tatatcaaaa attcttaaat tttttattat ttcgaagctt tgatctgctg tttttgcaaa
     4321 accaattccg ggatcgatca ctattttatt aacgttgagt ttatctaaaa cggatattct
     4381 cgatctaaaa tactccgtta tttcttccag gagattacta taatcagtga gatttttcat
     4441 cgtttgaggt gtgccacgca tatgcataag tacgtatccg acattttttg aggccacaag
     4501 attgaacatg tcaggatcgc cctcaccacc ggtaatatca tttaccatat ccgctccttc
     4561 ttcaatagca gcactggcta cttcggctct aaatgtgtca attgagatat gagttccggg
     4621 aaaatgtgag attatgttcc ggacagccgg aataactctt tgcaattctt cttccagact
     4681 gatgtgctcc gctccgggtc gtgaggagta tccgccaaca tctaatatgg aagctccttc
     4741 ttcaaggtat tgtccggcct gggcaattat ggcatcacct gactttctgc ttttttcgta
     4801 aaagctatcc ggggtaatat taagaatacc catgcagatg ggagtcgtca ggtcaatcac
     4861 atcgccgccg agggtaagcg ttttttttga aaataatgta tcttgctcca ctataaaaat
     4921 attgatcaaa aagacgtgtc tgatactgtt cgcgaatata atgaagtaat aaagggttgc
     4981 aaagagcttt tcaaaaagaa aacccgcgac tacggaacag catggagaat tttacggctt
     5041 ccctcactaa ccgatcaaat ctttatcaaa gcgcaacgca tccgatctat tcaggagaaa
     5101 ggagtttcaa aaattgccga aggaattcag gaagaactga tagggatcat taattattgt
     5161 gtaatgtcta ttatgcaaat ggaatttgag gaaaaagata aagttgaaat gacatacgaa
     5221 gagctttcac ccatttatga caggataaca aaggaaacaa tggagttatt catggataag
     5281 aatcatgatt atgctgaagc ctggagagaa atgagggttc catccattac agatattatt
     5341 ttgatgaagg ttttgcgggt gaaacagata gaagataatg agggcacgac tcttgtatct
     5401 gaaggggtga agtcaaatta ccaggacatt gtgaattatt ctgtattcgc gctaataaaa
     5461 ttaaactatg agaataatga aaatagttga ttggattgca aaaataatcg ttggcgggct
     5521 tttcatcttt tcaggcttga ttaaaattaa tgatccggtc ggaacgcaga ttaagctgga
     5581 agaatatttt gaagtttttg ctactgactt cggaagtttt tttcacttat ttatgccgat
     5641 tgctttgcca atagcattta tactcatcat acttgaagtt gtgctgggcg ttgcggtact
     5701 gattaatttt agaatgaaga tttcttcctg ggttttgctg atactcattt tcttttttac
     5761 attccttaca ttttattcag catattttaa taaagtcaca gattgtggtt gttttggcga
     5821 tgcaattcca ttaacgccgt gggagtcgtt ttataaggat cttattctgg ttgttttgat
     5881 cgcttatatt tttgtccgaa gaaagcttct tgatgcgcta acgggcgaaa aaacgggcaa
     5941 ctttaccata tcgggaaccg cattggctac tttgatcatt gggatttatg ctgtcaggca
     6001 cttgcctttt atcgacttcc ggccatataa aattggagat aaccttatgg ttaatatgca
     6061 gccgcgggaa gaaccaattt ttgaatatac tttccggaaa gatggtgaat tgataaaatc
     6121 agaacaatat ctttcagagc aagagggcta cgaatacatc ggatatgaag taaagaactc
     6181 taaaaatacc attccaaaaa ttacggattt cagtgtttgg aatgatgaag taggtgaata
     6241 cactgatttg gcattgaccg ggaataaatt tatacttatt gtacaggacg cgggaaaagc
     6301 ttcccgcaag gatttagcta agattggtaa tttaatgaag acggtgactt tgcaagccga
     6361 agtactggtg ttcacatctt caggcgttgc cagtcttcag gattatcttg ttgaagcacg
     6421 ggttgcagtt cccgtatttt ttgttgacgc gaccgtattg aagaccatga ttcgctctaa
     6481 cccgggtatg atgttaatga aagaaggcac cgtgctgggt aaatggcatt ataacgacat
     6541 tccggttccg gaccaggtat taaatctttt gtagtgaaaa tctatctttt aggaatgccc
     6601 ggcagtggta aatctactat cggtaaaatg ttggcttctt acctgggctt aagcttcatt
     6661 gatatggata tggaaattga aacggaaacc gggaagtcca ttaaagaaat ctttgaaaag
     6721 aaaggcgaaa agcatttccg gttcctggaa aaacaactct tacaaatgct tgcaggtaag
     6781 gattctattg ttgtagcaac agggggcgga gtccccattc acaataacaa tatgaagctg
     6841 attaatgaga ccggaatctc tgtttacctg aaagcaagta tcgaacaatt acaatctaat
     6901 ttggaagaga gttactctgc aaggccgcta cttcaaaaga aaaatcttaa agattccctt
     6961 atgcaactat taacagagcg tgaagaagtt tattcaaaaa gcaggtatat aattcaggta
     7021 tcagaagatc caaaatcaac agttcgggaa attgttgaga tttttaaaaa ttgaaaccgg
     7081 cagttattaa ggctgaaact cttttattct ctactgtatt atacacggag gaagcaaacg
     7141 gacgaattga ggagtcgtat ttgcttgtta taatacctaa gtcaacataa aacttaggca
     7201 agcgaattcc gccgccaaaa gtaatgttgc ttcgcgaaga atcaacagag cttgatttcc
     7261 tggggtcatc aaaaaaggca aatccaccgc gaagtctgaa aatccctctg cgaaactccg
     7321 cacctagctt atagttcgtt acaaacccga actggtcatc aataagttga ttgtcgtcca
     7381 cagtttcaaa atcatcagaa cttatatgat tgtaagaata attgaggaat tcaacttcac
     7441 ctgtaataaa cccatgtttg ccaataaaaa cagacgcacc cgtattaaac ttcaaaggtg
     7501 ttcgtaaatt atactggctc acaaaaatat cacttagcgc ttcttctgta gtaagttgtt
     7561 tgttttccgg aatgtagaaa taattgtcaa aggtagaagt aagttttgct tcatataaat
     7621 cttcgaacac atagaagtta ggagtggaaa gtgacgcacc caaaattaac atgtcaaccg
     7681 gtctcaccat tatcccaaag gtcgcattga ttcctgctcc tgtaatgttc aaatcttctt
     7741 caagagtaaa ttcactcaga gatccattgg accggaattc tgaatacctg gaattttcat
     7801 ggtaatcgag tgttaacagg ccaatggttg ctcccaggta aaccttatct ccaaaatttg
     7861 ctccgtaggc aatgtttaca gaatattcgg agccttttga aattcttgtt tcccgctgca
     7921 ggtcaggata gtccagagtt tcgatatcag aagccacata atcatagtac tgaatcagat
     7981 ttccagaacc gtcatcaatt tcaaataagt caaccagata agcttcaaaa gcgaggtctg
     8041 agaaggtatc ttcattgccg ttatcaaatt tgtcgaaaac aaaatcaaag taatcgacat
     8101 ccacattgtc tttatccctt tgataggtaa ttctgttatt aaaatctttt gtcttggaaa
     8161 tcgataagcc aaaggatcct cccttcagga actcattatt ttcgattggt ctgtggaaca
     8221 caacgccaag gttagcgaaa ttaagcacag ccctgctgtc ggacaccgac tggcttctgc
     8281 tgtttccgga gagttccagg taagaatcag cagaattaaa attcaaagcc ggggtaaaac
     8341 tggcttcaga acgattgaaa aagccgaggc cggcaggatt tgaaaatgcc gagcttatat
     8401 ctccacccaa agcaacctgc gaaaacccga gagattgaat tctcgcgcta cctgatacag
     8461 gcgactggct gaatagcaga acttcgccgg ttaacccctg tgcaaaatgc cgacctggcc
     8521 acaatccacc cacgcttatc aaaataagca aacttgcctt tctcatgttt ttaaccttta
     8581 gtttttcctt gcgggtctgc ttgaactgga attattattt cctgaacggg tgcctgaaga
     8641 tttttcagta gatgatctgg caggtgctac attgctcctg gccggcgcag tgctccgtga
     8701 tggagttgaa ctcctgttaa tctggtaatc tctgttagaa cccggcgcag ttctgttcca
     8761 ttgctggtta gaattctgcc tgttgaaatc ctgagagctt ctttgtcttg tattattgtt
     8821 agtattaggg tttacctggt aaccagaaga acgctgatta gttctgacat catacaaact
     8881 gctgcggtta gtttttggtc gtacataatt cgtggatgta cgagtacttt gcgttgatct
     8941 ctggttgata atctgcggag tggtccggga tgcggagttt ctgtctattt catctctgga
     9001 gataatctgg ttatcctgtc ttgacctcct gtaataactg ctttgagaag gcgcgtctgt
     9061 gatggtttga gttctggacc ttgatggatt agtaatatta ctcacggtac tccttgagtt
     9121 attattttca gtcagggaag aaacaacacc ccggcttggc ctgggacctt ttactatctt
     9181 gcgcgtatta ccattaatat tatccgggtt gatattaaca tagctgttat tgtaataaag
     9241 ggaattggaa tagccgtatg aactatagta tccgccacca taataactgc cgtaataaaa
     9301 tccagatgga gggcaataca ttgccgcata tacccagctg gggctgtagc cataataaga
     9361 atcatacaga taccagttgt tccagcgatt atatccccat ccataactat acgaaatggt
     9421 cacccgaatt ccggagccat agtaatatcc tgaatagaat ggatcccaat agaatggatc
     9481 ataataccgg taattgtatc gatatggaat tccataacca cttccgtaat aattattgat
     9541 tattgttgac ttttcatcct ctgatgaatc gtatccttcc gcataataat caccttcccc
     9601 gtattcgctg ccctcgtcct cagacaattc agaattatat ttagaaatat aatcaggatt
     9661 taaggttttg gctgaataat tatcaacagt caaatcttct tcgccatcca gttttaaagg
     9721 attggcatct tccctgaaac ttgaatatgc attgaaatta acatccttgt atgattcgcg
     9781 ctggcgatca gcggaaccat aatacatatc atcaacctct ccttgatgtg ccaactgaga
     9841 agagctacaa gcacctaaaa taagtgccga caaagccgct accgttaatc ctgtgattcg
     9901 tttcatggct gttttgttta tcgttttcac ttaaccaaat ttacgattat tttaatcgct
     9961 gggaggcgca taaatacttt atatttgtcc tccgttttaa tacaagtcca aaactatgcc
    10021 aaagtcaaaa tgagtaaggg aattacgacc agggaagcgg attattcaca atggtacaat
    10081 ga
//
LOCUS       DASWHX010000133         4923 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_1223719, whole genome shotgun sequence.
ACCESSION   DASWHX010000133 DASWHX010000000
VERSION     DASWHX010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 4923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4923
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1223719"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..874
                     /locus_tag="VGA21_09140"
     CDS             <1..874
                     /locus_tag="VGA21_09140"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEX9650714.1"
                     /translation="GINTEGPAAALDGSIFFVDVPISQLHDSVTAGRIWRFDHQTGKS
                     TVYRSPSGKAIGLKFDADGRMVVAEFADFGGRRITRTDLATGKAEIIAALFQGKPFNG
                     PNDLTIDEKGRIYFTDYGYNAPHEVIYHRGDGVYRIDPKGTIERIINHAGKPNGIAIS
                     PDQKTIYIASNQLDIFGTSAILAYDLSNDGKVVFRSIFEQFEPNTVLPDGMTFDTEGN
                     LYVALFSRIARTGVVVYSPKGKEIAFIPTPERAKNVAFGRGNESNLLYITAGKSLYRI
                     SVTKTGYQLPPIQR"
     regulatory      1172..1393
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            1550..3766
                     /locus_tag="VGA21_09145"
     CDS             1550..3766
                     /locus_tag="VGA21_09145"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX9650715.1"
                     /translation="MLNTNKRKKGNVKSRYLSGKHKAILVVTMLVASRMSHAQQVDTI
                     MTMELQPVTITATRSEKNPIDVGRSVTVISNADIKNSGANSLAEFLTMQEGIYVVGGQ
                     QNPGSLNNLSMRGANGNQTVIMVDGIRITDPTTTDNSINLSELSLSNVDRIEIVRGSH
                     STLYGSSAIGGVINILSKKNNEKTGIHTDAELKGGTFNSNGSVLSENVFINYTLKNGF
                     YVNAEMNNSVSKGFNSTTDTVTNLNSYKHPEMSDGSQKLDLIGKLGYNAKNLDMHASF
                     KQVKQSVDIDDGLFQDDDNYIIKFKRNLFTYGASYKFNDKFSVSYYGGMSNMERKAID
                     DSSQVDNIGTTDHSYFSGKYNGSIASNDLQFNYHIKGIDLVAGGNIYKETMTAQNYYY
                     STAFGVYESRSNWDSLKINTQTANGFIYADLNGILLNEKLSVLSFGVGGRYVSHSLFG
                     NVFTYEINPSLKLSKEALLYFSNTTGFNAPSLYQLYSPDTDFSSGITRGNKTLKPEES
                     QSFEIGIKQKLNNQFWYSIAYFNTKVKNVVDYVYLWDKSISVDSLSFGDYKGDTYINL
                     GTQYAHGFEFSLTSKITEKLLLSGNLSIVQGKIKYNASAIDTMHTRGNHIQLFSSGSF
                     VTKETESIGLVRRPSTANVFITYTPVKKLALRVDARFAGARSDIYYDSALGPFGALNT
                     VDVEDYTLLDFSARYEIIKGFTAMVRIENIFDRKYTEIRGYATRGRGIYGGVRYSF"
     gene            complement(4315..4710)
                     /locus_tag="VGA21_09150"
     CDS             complement(4315..4710)
                     /locus_tag="VGA21_09150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650716.1"
                     /translation="MAEYLFRGGYKNRAVEVKVNLLLFHFKDESNIHFIYSPHLDLTG
                     YGQNLKDAKKSFEIVFEDFIDYTLKKRTLGKILTNLGWELKGTLKRPKKVLAPSITSV
                     ISQNDYVSEIFDKYPVNTFHQEVGIPAFA"
BASE COUNT         1584 a          924 c          936 g         1479 t
ORIGIN      
        1 aggaattaac acagaaggtc cagcagcagc acttgatggt tctatcttct ttgttgacgt
       61 gcccataagt cagttgcatg atagcgttac agctggtcgt atctggaggt ttgatcatca
      121 aactggaaag tctacagttt atagatcgcc cagcggaaaa gcgatcggtt taaaatttga
      181 tgcagacggt agaatggtcg ttgcagaatt tgctgacttc ggtggcagac gcatcacccg
      241 cactgatctt gccaccggaa aggctgaaat tatcgctgcg ctgttccaag gaaaaccttt
      301 caatgggccg aatgatttga caattgatga aaaaggccgt atctatttta ctgactatgg
      361 atacaatgct ccccatgaag ttatttatca tcggggtgat ggcgtatata gaattgatcc
      421 caaaggaacc attgaaagaa taattaatca cgctggaaag ccaaatggta tcgccatttc
      481 tccagatcaa aaaactattt acattgcttc aaatcagctt gatatttttg gtacatcagc
      541 aatcctagct tatgatcttt caaatgacgg caaggttgtt tttcgttcta tatttgaaca
      601 gtttgaacca aacacagtcc ttcctgacgg aatgactttt gatacggagg gcaatttgta
      661 tgtggcccta ttctcaagga ttgctcgaac aggcgttgta gtttattctc cgaaaggtaa
      721 agaaatagca ttcattccaa cacctgagcg tgctaaaaac gttgcatttg gccgaggtaa
      781 tgaaagcaac ctcctttaca ttactgcagg gaaaagtctc tatcgtataa gtgttacaaa
      841 gactggttat cagttgcctc caatccaacg ataatatgtg attccgacaa aatgggattg
      901 acagtttact aatgttgtta agtaaccgaa agaccaaata agaattattc caggccgaca
      961 ctgctgacaa gaagacaaat ggataaagta ataaacagga gatgtagcct tccccttttt
     1021 aggaccactc taaaatcgac attttttatt ttcatagcca acccaacagc caatcacaat
     1081 accgcgccaa taaatattat ccaaaacaaa aaattcttag agaatctcaa aaactttata
     1141 tctttgccct gttaaacgtt ctttctgttg ttcaatcatc tataatttag gtgttccgat
     1201 ttcaaacctg tctgccgcag gaaggtcgga aataataggg aaccgtgtga gaatcacggg
     1261 ctgtcgcgca actgtaatct cgtcccaata catagggaaa gcgcctctga taaattaatg
     1321 ccactgctta aatgtgggaa ggcaatcaga agtcgggaaa gtcaggagac ctgcctaaac
     1381 tgtttgatga ctttattgct tccgcgaaat ggagcatagg tcaggttaga tggaattttc
     1441 cttcccgata catcgggctt ccattaatta tcctttctta ttctgttttt tggtttgcaa
     1501 taaacgaaca actgttattg cattattaac cctttaaaac caaaaaacaa tgttgaacac
     1561 aaacaaaaga aaaaaaggaa atgtgaaatc ccgatattta tcggggaaac acaaagcaat
     1621 ccttgtggtt acaatgttag tggcttcgcg aatgagccat gcacaacaag tagacacaat
     1681 aatgacaatg gagctgcaac cagtaaccat cactgctacg cgcagcgaaa aaaatccaat
     1741 tgatgtaggt cggagcgtaa ctgtaatcag taacgcagac atcaaaaact caggagctaa
     1801 ctcattagca gagtttctaa ccatgcagga agggatttat gtcgtgggag gtcagcaaaa
     1861 ccccggtagt ttgaacaatc tctctatgcg tggggcaaac ggcaatcaaa ctgtgataat
     1921 ggtagatggc attcgaatta cggatcccac gacaactgat aattccatta atctctcaga
     1981 actttcactc agcaatgtag accgcattga aattgtgcgc ggttcacaca gtacacttta
     2041 tggttcatcc gcaattgggg gtgtaatcaa tatcctttct aaaaagaata atgagaagac
     2101 ggggattcat actgatgcag agttaaaagg cggtactttc aatagcaatg gttctgttct
     2161 ttcagaaaat gtatttataa actacacgct taaaaacggc ttttatgtaa atgccgaaat
     2221 gaataactca gtcagcaaag gatttaattc cacaactgac actgtcacca atctgaactc
     2281 ctacaaacat cctgaaatga gcgatggttc ccaaaaactt gacctcatcg ggaaattggg
     2341 atataatgca aaaaacttag atatgcatgc cagctttaaa caagttaagc agagtgtaga
     2401 tatagatgat ggcttgtttc aggatgatga caattacatc attaaattta agcgtaacct
     2461 gtttacctac ggtgcttctt ataaattcaa tgataaattt tctgtgagtt actatggggg
     2521 tatgagtaat atggaaagga aagccattga tgattcttcc caagttgata atatagggac
     2581 aaccgaccat tcttattttt cagggaagta taatggttca attgccagta acgacttgca
     2641 attcaactat catataaaag ggatagacct tgttgcgggt ggaaatattt ataaagaaac
     2701 catgacagcc caaaactatt attattcgac agcttttgga gtttacgaat ccaggagcaa
     2761 ttgggattcg cttaaaataa atacgcaaac agctaacgga tttatttatg ctgatttgaa
     2821 tggaattttg ctgaatgaaa aattatcagt gttatcattt ggcgttggag gaagatatgt
     2881 gagtcacagt ttgtttggca acgtatttac ctatgagata aatccatcgc tgaaactttc
     2941 gaaagaggcg ctcttgtatt tttcaaacac cacaggattt aatgcgccat cgctttacca
     3001 attatattca cctgatacag actttagttc gggtattacc cgcgggaata aaacgctgaa
     3061 gcccgaagaa tcacaatcgt ttgaaatagg aataaagcaa aaattgaata atcagttttg
     3121 gtactcaata gcctatttta atacaaaggt aaaaaatgta gtggactatg tttacctgtg
     3181 ggataaatca atctccgttg attccctatc cttcggtgat tataaaggag acacttatat
     3241 aaatcttggc actcaatacg ctcatggctt tgagttttcc cttacgtcaa aaataactga
     3301 gaagctatta ctatcaggta acttgtcaat tgttcagggg aaaattaaat acaatgcatc
     3361 agctattgac actatgcata cacgaggtaa tcacattcag ttattttcaa gcggttcatt
     3421 tgtcacaaaa gaaaccgaat caatcggctt ggttcgcagg ccaagcacag cgaatgtctt
     3481 catcacttat acgcctgtaa aaaaacttgc gttgcgtgtt gatgccagat ttgcaggagc
     3541 gcgtagtgat atttattacg attctgcact tggacccttt ggtgctttaa atacagttga
     3601 cgtagaagat tataccttgc ttgatttttc agcgcgatat gaaatcataa aaggatttac
     3661 agctatggtt cggatagaaa acatttttga taggaagtat actgaaataa ggggatacgc
     3721 aactcgcggc aggggtatct acggaggagt cagatattca ttttaaaaac taacaactaa
     3781 ctacataacc aatgaaaaca aaaatcaatt ctgtttatta tcatctcctt ttaatagttg
     3841 tagcagacgc cactcctgtt taagttttcg gtttgtgagt tggatgtggg tgacttaaca
     3901 agccggttga aaggtataat aaggtataat ggagaaatgt tatatcaagg ctatgtccaa
     3961 ctcagttttt attcaccatt tctccaaagc ctttagttta cttttcgctc catcatttct
     4021 cactgttttt tgttattatg tagttgaatg caatcagagc ttcaacattg ctttaaaaaa
     4081 atcatttcta tgcatctcta aatgacgaat gacttgcttt actataaatt ctggaacggg
     4141 gtcaatatgg gtttgaattg taatcggtct atccattcct gatttcgacc acttttcgtg
     4201 accccctctg ccttttgtgt cttttattac atttaagcct tgacttatta aaaactttcg
     4261 gaaatccttt aacgggatat tcgataactt tttagtcgac atcggaattg agaattatgc
     4321 aaatgcaggt attccgacct cttgatgaaa agtatttacc ggatatttat caaatatttc
     4381 cgaaacataa tcattctgac ttatcaccga agtaatgctt ggagctaata cttttttagg
     4441 tctcttcaaa gttcctttta attcccatcc taaattagtc aagattttac ctaaagttct
     4501 cttttttaaa gtataatcaa tgaaatcttc aaatacaatt tcaaatgact tttttgcgtc
     4561 ttttagattc tgaccatatc ctgttaaatc caaatgtgga gaataaataa aatgaatatt
     4621 actctcatct ttgaaatgaa aaagtaataa attaaccttt acttcaacag ctctgttttt
     4681 ataaccgccc cgaaaaagat attctgccat aattttttta gaaaggattc aaatgtatgt
     4741 aaatataaat aactattgcc aataataggg cttcgtgtgg acagaacagc ctgctccgat
     4801 cattttatcg gggacccaac atgaagctct cattctggct taagcctaaa acagaaatgg
     4861 acaagtttgt taactggttc aacaataacg ataaaacaga cccggtaata aaagcagcca
     4921 ttg
//
LOCUS       DASWHX010000134         9769 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_857532, whole genome shotgun sequence.
ACCESSION   DASWHX010000134 DASWHX010000000
VERSION     DASWHX010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 9769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9769)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9769
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_857532"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1017
                     /locus_tag="VGA21_09155"
     CDS             <1..1017
                     /locus_tag="VGA21_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020529458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEX9650717.1"
                     /translation="TIAILPILGIGGMQLFMAEAPGITADKLKPRIQDTATRLWLIYV
                     MLTGFEFMLLMAGGMSFFDSINHSLTTMSTGGFSTRQESIGYFGSPFVHYVIIAFMIL
                     GGSNFTLTYFLLKGRIQKLFRNDEFKYYILIVLLFTVTTFIGLLIIGQFGFEESFRNS
                     LFHVVSIVTTTGFVTGDYTAWGPFLKILFFSMMFIGASAGSTAGGVKIVRHIILFKNS
                     FLEMKRQLHPSAIIPIRYNGHAVDREISYNVQAFIMIYFTTFAIGSLVVSAFGIDFET
                     AMGSVASTLGNIGPGIGTVGPIYSYAHFPDAVKWILSFLMLLGRLELFTVLILLTPYF
                     WRMR"
     gene            complement(1093..4119)
                     /gene="secDF"
                     /locus_tag="VGA21_09160"
     CDS             complement(1093..4119)
                     /gene="secDF"
                     /locus_tag="VGA21_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009184900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecDF"
                     /protein_id="HEX9650718.1"
                     /translation="MRNKGAIVALTLIVTALCLYYLSFTLVSRGIQKDATNYAIDSTG
                     IVDFSKKQKYLDSIWTEPAYNLLGISYTFQEVKENELNLGLDLQGGMHVILEISPVDI
                     IKGLSGNNEDPNFTQAINEARIAQRSSQSRFVNLFYSAYKKLEPNKPLAQIFATAANR
                     GRINFDTPDDDILTILDEEVESAIDRSFNILRTRIDRFGTSQPNIQRLQNTGRIQIEI
                     PGADNPERVRKLLQGVAKLEFWEVATPDEFQNSLLAINNMLVQEQEKLRLIERDLKGN
                     IEEPPQEDLGNLLSTENQDDSLADDSAEDLASQLEGDTTATGLDSLTSNQSPIFTLLQ
                     TGDGTLVYNVKDTSKINAIFKRDEVQSLMPRTLRIFWDVKPHDHEGDTGEPHLELHFV
                     KSARSGQAPLTGEVITDARQDLDEASRPSISMQMNATGAKAWKKLTGDNVGRRIAIVL
                     DSYVYSAPTVQNEIPNGNSQITGNFSLDEAKDLANILKAGSLPAPTRIVQEVIIGPTL
                     GKEAQRQGIISIVSGLVLVIVFMVAYYARGGFVANFALIFNIFFIMGILAQLNAALTL
                     PGIAGIVLTIGMSIDANVLIFERIREELRNGRPLREAIDNGFRKAYSSIIDSNVTTFL
                     TAVILNWLGQGPVKGFAITLMIGIACSFFSAVFITRVIIDWMVARKGDKTKLSFRTPL
                     SGSLLQDLSIDFLGKRKIAFIISALLISGGVALISINGLNLGVDFKGGRSYVVTFSEP
                     VAASEMKSELTRAFEGNGTEVKTFGGSNIIKVTTSYLVDDESDSSDVKVERALINGLT
                     NITGKEYERDDSKVNNNTFAISSSEKVGASIADDIKNASMESVALSLVVIFLYIVIRF
                     RRWQFGLGAIIALFHDTLAVLSCYAIAEALGFSFEIDQVFIAAILTVIGYSINDTVIV
                     FDRIRETLGLKTSSDIEKTVNTAINNTLSRTMITSLTTLLVITVLLLFGGEVLRGFSF
                     SLFVGIVIGTYSSIFIASPIVVDLQKRKLSKAPATS"
     gene            complement(4184..4537)
                     /locus_tag="VGA21_09165"
     CDS             complement(4184..4537)
                     /locus_tag="VGA21_09165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650719.1"
                     /translation="MKKVPEPLKSTTATLIFGVLVVFFLYSSSFPESNRNISNPGQTE
                     QSQADDESEQDHLLISGDWVTVTVSPQMQPILKWIRDIDFLENPVIHVHEIIGTGSTQ
                     FFQTLFQRIISPNAP"
     gene            complement(4580..5197)
                     /locus_tag="VGA21_09170"
     CDS             complement(4580..5197)
                     /locus_tag="VGA21_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002699134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HEX9650720.1"
                     /translation="MKSTFVVGITGCSGSGKTFILNSILSHFHTDEIAIVSQDNYYLP
                     GEKVPMDENGIINYDLPESIHLGGFQEDIIKIKQGERLSKMEYTYNNPAKSPRLIETT
                     PAPIIFVEGIFIFSHEIIDNELDYRIYVEAESNIRLNRRIQRDLTERGYDSDDVNYRY
                     RNHVMPAYKKYVEPFRHKADLIIENNDLTTPVLHDLVTFLKTKSR"
     gene            complement(5212..5799)
                     /gene="rdgB"
                     /locus_tag="VGA21_09175"
     CDS             complement(5212..5799)
                     /gene="rdgB"
                     /locus_tag="VGA21_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014799767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HEX9650721.1"
                     /translation="MLEICFATNNQHKIKEVQALLPLSIKLLSLEEIGCLQELPETQN
                     TIEGNSRQKAEFVFNNYKLNCFADDTGLEVQALKGKPGVLSSRYAGIPANPEKNIQKL
                     LQELRNEVNRKARFKTVVTLLLNRNSKQFEGILNGKIIDNLRGDNGFGYDPVFLPAGF
                     EITLGEMDSSLKNKVSHRGIAIGKLISFLSNIFPS"
     gene            complement(5809..6741)
                     /locus_tag="VGA21_09180"
     CDS             complement(5809..6741)
                     /locus_tag="VGA21_09180"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEX9650722.1"
                     /translation="MKKIIAFVILAFLTSCTNTQYQKTETGIEYKYLSQSSSDIPSDG
                     DIMELHLIYKDENGKELLNTTVDSDGPIPVLCQSEKFIKNGSLEEIFGMLKAGDSIEA
                     KIPAARVFESFGQPVPDSIDANSNLIFNIGVVNVMNRQEYTQSIAEKYMASAKIQLAL
                     SLEQIQTDGEIIDQYLAKNKIQAERTESGLRYVITQSGDGNKAKPGDRISVNYVGMVL
                     DGRVFDTSIKEVAQDNNVYNPGREPYQPYPFTLGLGEVIYGWDEALTLLNEGAKATLY
                     IPSSMGYGNQPVDEIIVENSILIFEIELVDIMEL"
     gene            complement(6749..7753)
                     /locus_tag="VGA21_09185"
     CDS             complement(6749..7753)
                     /locus_tag="VGA21_09185"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009580563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HEX9650723.1"
                     /translation="MHNIREFKDLLSSPKKIAIIPHQKPDADALGSALGLSNYFIKKG
                     HQTHVLAPTDYPDFLIWMKGNETVINFEKDPELGIKYVNESDIICCIDFSSLKRIEKL
                     GEIVEKSAAVKVLIDHHLEPDDFADFSFSNTKASATAEIIFDMICELGDKSLIDRDIA
                     EELYAGIMTDTGSFHHSNTTQKVHRVAAELIELGADVTRVAKLVYDTNSLDRLRYIGF
                     ALSNSLHLISDLNAAYFSISMKDLEKFNSKTGDTEGLVNYGLSIEGIQVSAAIIERPD
                     MIKFSFRSIGEFSVNDFARKYFNGGGHKNAAGGKLIGTLDEAVQKFKTALKEFHSHNN
                     "
     gene            7908..8336
                     /locus_tag="VGA21_09190"
     CDS             7908..8336
                     /locus_tag="VGA21_09190"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009187047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEX9650724.1"
                     /translation="MSGNRTFTMIKPDAVSAGNSGAILKMIEETGFKIIAMKYTKLTK
                     ERAGEFYAIHKERPFYTDLCNYMSSGPIIPIILEKSNAVEDFRKLIGATNPKEAAAGT
                     IRNLFAESIEANAVHGSDSDENAEIEADFFFSKTERFQEN"
     gene            complement(8333..9082)
                     /locus_tag="VGA21_09195"
     CDS             complement(8333..9082)
                     /locus_tag="VGA21_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014019718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEX9650725.1"
                     /translation="MSYEIKLPLFEGPFDLLLFFIERDELDIYDIPISQITKDFLDYI
                     RHLETMNMEVASEFILVAATLMRIKAKMLLPRPVLDEEGKEIDPREELVKHLLEYKKY
                     KSVLSELAKFEEDRSQLESRGNITRELKSLSENANVEWELLNLDLYKLLKVFQRVMER
                     YEYELNRPSHKVIQWPYTLEQQKKYILQKVGKESRVSFSQIIEDNPTKIAVIFNFLSI
                     LDLLQLKLITMHIGIGFNNFWIESFEAVRSN"
     gene            9227..>9769
                     /locus_tag="VGA21_09200"
     CDS             9227..>9769
                     /locus_tag="VGA21_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009580827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase
                     N-terminal domain-containing protein"
                     /protein_id="HEX9650726.1"
                     /translation="MLIKPGPLLAQINSPDDVKKLDAIQLVQLCEELRQFIIDNVSVY
                     GGHFGASLGVVELTVALHFVYDTPKDQLVWDVGHQAYGHKILTGRRENFSTNRIFKGL
                     SGFPKRKESEYDTFGVGHSSTSISAALGMAVGNKYKHDPRHHIAVIGDGALTGGIAFE
                     AMNHAGVSNTDLLIILNDNCM"
BASE COUNT         2867 a         1973 c         1886 g         3043 t
ORIGIN      
        1 acgatagcga tactgccaat tttgggaatt ggaggaatgc agttatttat ggcagaggca
       61 cccggaatta ctgccgataa attaaagccc cgaatccagg ataccgctac cagattatgg
      121 ctcatttatg tgatgcttac aggttttgaa ttcatgttgt taatggctgg gggaatgtca
      181 ttttttgatt caataaatca ctctctgacc acaatgtcta ccggaggatt ttcaacgcgg
      241 caggaaagta ttggttattt cggaagccca tttgttcatt atgtaattat tgcattcatg
      301 atcctggggg gctcaaattt cactcttaca tactttcttc taaaggggcg gatacaaaag
      361 ctgtttcgaa atgacgaatt caagtattac attttaatcg tcctgttatt taccgtgact
      421 acattcatag gattattaat aatcggacaa tttggatttg aagagtcgtt caggaattct
      481 ttatttcatg tagtatcaat tgtgacaaca acaggatttg taaccggcga ttacacggct
      541 tggggacctt ttctcaaaat tttatttttt agcatgatgt ttatcggagc ttcagccggc
      601 tcaacagcag gcggagttaa aattgtgcgt catattatac tttttaagaa cagcttcctt
      661 gaaatgaaaa ggcagttgca cccatcggct ataattccca taaggtataa tggccacgcg
      721 gttgacaggg aaatatcgta taatgtgcag gcatttataa tgatttactt cacaaccttt
      781 gccataggtt cccttgttgt ttcagctttc ggcattgatt ttgaaaccgc tatgggttca
      841 gtagcttcca cacttgggaa tataggacct ggtattggta ctgttggtcc aatttatagc
      901 tacgcacact ttcctgatgc agtaaaatgg attttatcat tccttatgtt gcttggaaga
      961 cttgaattgt ttacagtttt gattcttctt acaccctatt tttggcgtat gcgataatgc
     1021 tttttaaggg aatcccttct cgggtcttca aaaataaaaa aggccgcccg aaaggggcgg
     1081 cttcaatcaa aatcaactcg tggctggcgc cttgcttaat ttccttttct gcaaatcaac
     1141 tacaattggt gaggcaataa aaatagatga atacgttcca attacaattc caacgaaaag
     1201 tgaaaaagaa aatcctctca gaacttcacc accaaagagt aatagcacgg ttattactaa
     1261 caatgtggta agggaggtta tcatcgtacg gcttaaagtg ttattgattg cggtatttac
     1321 cgtcttctca atgtcagagg aggtttttaa tcccaaagtt tccctgattc tatcaaatac
     1381 aatcacagta tcattaatac tgtatccaat tacggtgaga atggctgcaa taaaaacctg
     1441 atcgatttca aaagagaatc ccagggcttc ggcaattgca tagcaagaca aaaccgccag
     1501 agtgtcgtga aataaggcaa tgatagctcc aagtccaaat tgccatcttc taaagcgaat
     1561 aactatgtac aagaagatta ccaccagtga aagggcaacc gattccatag aagcattctt
     1621 gatatcgtca gcgattgatg ccccgacttt ttcagaactg gagatcgcaa aggtgttgtt
     1681 gttaactttg gaatcgtccc gttcgtattc tttaccagta atattggtta acccgttaat
     1741 aagtgcccgc tcaactttaa cgtctgaaga gtccgattca tcatcaacca aataactcgt
     1801 tgttacttta attatattac tgccgccgaa agttttcact tctgtgccgt tcccctcaaa
     1861 agccctggtt aattcagact tcatttcgga cgcagcaacc ggttcactaa aagttacaac
     1921 ataggaccgg cctcctttga aatcaacgcc caggttaagt ccatttattg aaatcagggc
     1981 tacaccgcca gagattaaca gcgcggatat tatgaacgcg atttttctct tacccaaaaa
     2041 atcgatggac aagtcttgta ataaagagcc cgaaagcgga gttctaaatg acaatttggt
     2101 tttgtctcct ttcctggcta ccatccaatc aataattacc cgcgtgataa atactgctga
     2161 aaaaaaggag caggcaatac ctatcattaa ggtaatagca aatcctttaa ccggaccttg
     2221 ccccagccaa ttaaggatta cggctgttaa gaaagtggta acattggagt ctataataga
     2281 actatacgct ttcctgaatc cattatcgat ggcctctctt aacggtcggc cattgcgcaa
     2341 ttcctcacga attctttcaa aaataagtac gttggcatcg atagacatac caatagtcag
     2401 cactataccg gcgattcctg gcaaagtgag cgccgcattc aactgggcaa ggatgcccat
     2461 aatgaagaaa atattgaaaa tgagtgcaaa gtttgccaca aaacctcctc ttgcgtaata
     2521 agccaccatg aacacaataa ctaaaacaag tccggaaaca attgagatta ttccctggcg
     2581 ctgggcttcc tttccaagag tgggcccaat aattacttcc tgtacgattc gtgttggggc
     2641 aggtagtgaa ccggccttca gaatgttagc caaatctttt gcttcatcaa gagagaaatt
     2701 tcccgtgatt tgagaatttc catttggaat ttcattttga actgtaggag ctgagtagac
     2761 ataactatcc agcacgattg cgattctgcg acctacgttg tccccggtta attttttcca
     2821 tgctttagct ccggttgcat tcatttgcat ggaaatggaa ggcctgctgg cttcatccag
     2881 atcttgcctt gcatctgtaa taacctcccc tgtgagtggt gcctgtccgc ttctcgcgga
     2941 tttgacaaaa tgaagttcca aatgcggttc tcctgtatca ccttcatggt catggggttt
     3001 tacatcccag aagattctca gagtcctggg catcaaactt tgaacttcat cccgcttgaa
     3061 aatggcatta atctttgatg tgtctttcac attatacacc aatgtgccat ctcctgtctg
     3121 gagtaaggtg aaaattggtg attgatttga agttaatgag tccaggcctg ttgcagtagt
     3181 atctccttcc aactgtgacg caagatcttc tgctgaatca tctgccaaag aatcatcttg
     3241 attttctgtt gataacaaat taccaaggtc ttcctgagga ggttcttcaa tatttccctt
     3301 caaatccctt tcaattaatc gcaatttctc ctgctcctga accagcatat tgtttatagc
     3361 aagcaatgaa ttttgaaatt catccggagt tgccacttcc cagaattcca gctttgcaac
     3421 tccctgcaag agttttcgaa ctctctccgg attgtcagcc ccgggaatct caatttgaat
     3481 cctgccggtg ttttgtaatc gctgaatatt gggttgtgaa gtaccaaaac gatcaatgcg
     3541 ggttcttaag atgttgaaag atctatctat tgccgactca acttcttcat caagaatggt
     3601 taatatatca tcatcaggcg tatcaaaatt aatcctacct ctgttagcgg cggtagcaaa
     3661 aatttgtgcc agaggtttgt taggctccaa ttttttgtat gcactataga aaaggtttac
     3721 aaatcgcgac tgactgcttc gctgggcaat gcgagcttca ttgatcgcct gagtgaagtt
     3781 gggatcttca ttattgccac ttaacccttt tatgatatcg acaggtgaaa tctccaggat
     3841 aacgtgcatc ccgccctgaa ggtcaagacc aagattcaat tcgttttctt ttacttcctg
     3901 gaaagtgtaa ctaataccaa ggaggttgta ggccggttcc gtccaaatgg aatccagata
     3961 tttttgtttt tttgaaaaat ccacgatacc ggtggaatct atggcgtaat tcgtagcatc
     4021 tttctgtatt cctctcgaga ccaaagtaaa ggagaggtag taaagacaga gtgctgttac
     4081 aattaaagtt aaggccacaa tggcaccttt atttctcatg atttattttt ttaattgcta
     4141 aaaagtaagg tagtgtaaaa ttcaatcgga ttgaatcgta aaactacggc gcgttgggag
     4201 aaataatccg ttgaaaaagt gtctgaaaga attgcgttga tcctgttcca atgatttcgt
     4261 ggacatggat aacagggttt tcaagaaaat caatatctct gatccatttc aggattggct
     4321 gcatttgtgg agaaacagtt acagtaaccc aatctccgct tatgagcaaa tgatcctgtt
     4381 cagattcatc atcagcctgt gattgctcgg tctgaccggg attgctgata tttctgttgg
     4441 actctggaaa cgagctggag tacaaaaaaa atacaaccaa aacaccaaaa attagtgttg
     4501 cagttgtgct tttcaatggc tccggaactt tcttcattgg gcgacaaaat tatagctacg
     4561 cgtagaaacg acaaaggaat taccgggatt ttgttttgag gaaagtcacc aaatcatgca
     4621 aaacgggagt agtgaggtca ttattctcaa tgatcaagtc tgctttgtga cgaaaaggct
     4681 ccacgtactt tttataggca ggcataacat gattcctata cctgtaattt acatcatctg
     4741 aatcgtatcc tctctcagtt aaatcccgtt gaatccgcct gttaagcctg atattactct
     4801 ctgcctcgac ataaattcga tagtcaagct cattatcgat aatttcatgg gaaaaaataa
     4861 aaatgccttc aacaaagatt atgggtgcag gggtggtttc gataagcctg ggagatttag
     4921 cggggttgtt atacgtatat tccatttttg acaacctctc cccttgtttt attttgatga
     4981 tatcttcctg aaaacctcca agatggatag attctggtaa atcataattg attatgccat
     5041 tttcatccat gggtaccttc tcgcctggta aatagtaatt atcctggctt acaatcgcga
     5101 tttcatcagt atgaaaatgc gataagattg aattaaggat aaatgttttt cccgagccac
     5161 tacacccggt tatgcctaca acaaaagtgg atttcatggc tcaaatttac ctcaggaagg
     5221 aaaaatattg gagaggaatg aaattaattt gccaattgca ataccgcggt ggctcacttt
     5281 gttctttaaa gaagagtcca tttctccgag agtaatctca aatccagccg gaagaaaaac
     5341 ggggtcataa ccaaacccgt tgtctcctct taagttgtcg attattttcc cattcagtat
     5401 gccttcaaat tgtttggaat tcctgttcaa taataatgta accactgttt tgaatcttgc
     5461 tttacgattg acttcgttcc ggagctcttg caaaagtttt tgaatgttct tttcagggtt
     5521 agcaggaatg ccggcataac gagacgaaag cactcctggt tttcctttca atgcttgaac
     5581 ttcaaggccg gtatcatcag caaaacaatt cagcttataa ttattaaaaa caaattcagc
     5641 cttctgacgg gaatttcctt caatggtatt ttgtgtttcc ggcaactctt gcaggcaacc
     5701 gatttcttca agagaaagca attttatgga aagcgggagc aatgcctgga cttccttaat
     5761 tttatgctga ttatttgtcg caaaacatat ttccagcata aaataaattt ataattccat
     5821 tatatctacg agttcaattt cgaaaatcaa aatagaattt tctacaatta tttcatcaac
     5881 cggctgattt ccatagccca ttgaactggg tatatagagg gtggcttttg ctccttcatt
     5941 gagtagagtt aatgcttcgt cccaaccata aattacttca cctaatccta aagtaaaagg
     6001 atagggctga taaggttctc tgcctggatt atacacgttg ttatcttggg caacctcttt
     6061 tatgctggta tcaaagacac gcccatccaa taccataccc acataattta ctgaaattct
     6121 gtcaccaggt tttgccttgt taccatcgcc cgattgcgta attacatatc tgaggccgga
     6181 ttccgttctt tcagcctgga ttttattttt agccaggtat tgatcaataa tttctccatc
     6241 cgtctggatt tgctctaatg agagagccaa ttgaatttta gctgatgcca tatatttttc
     6301 agcaatgctt tgtgtatact cctgacgatt catcacattc accactccga tattgaagat
     6361 aagatttgag ttcgcatcaa tactgtcagg aacaggttgt ccaaaacttt caaacaccct
     6421 ggctgcaggt atttttgctt cgatactatc tcctgcttta agcataccaa atatttcttc
     6481 cagacttcca tttttaataa atttttctga ttgacataaa accggaattg gtccatctga
     6541 atcaacggtc gtatttaata actcttttcc attttcatcc ttgtagatta agtgtaactc
     6601 cattatatct ccatcggacg gaatatcaga agaagattgg gacaggtatt tatattctat
     6661 accggtttca gtcttttgat actgcgtatt ggtgcaagat gttaaaaagg caaggatcac
     6721 aaaagcaatt atttttttca ttgtgttatt aattattatg ggaatgaaat tccttcaatg
     6781 ctgttttaaa cttttgaaca gcttcatcaa gcgtgccgat aagttttcca cctgccgcgt
     6841 ttttatgacc tccaccgtta aagtattttc tggcaaaatc atttactgaa aactccccta
     6901 ttgatcgaaa tgaaaattta atcatatccg gtctttcaat aattgcggct gacacttgaa
     6961 ttccttctat tgataaacca tagttgacca ggccttcagt gtctcctgtt ttactgttga
     7021 atttttcaag gtctttcata gaaatagaaa aataagcggc atttaaatcc gaaatcaggt
     7081 gaagggaatt gctaagagcg aacccaatgt atctgagcct gtcaagtgaa tttgtgtcgt
     7141 acacaagttt tgctacccgg gtgacatcag ctcccagttc aataagctcg gcggctaccc
     7201 ggtgaacctt ttgggttgta ttggaatgat gaaaagaacc ggtatcagtc ataatacctg
     7261 catacaattc ctcagcgata tctcgatcaa taagagactt gtctcccagt tcgcaaatca
     7321 tgtcgaaaat aatttctgca gtggcggaag ctttagtatt tgaaaaagag aaatccgcga
     7381 aatcatccgg ctccagatgg tggtcgatga gtacttttac tgctgctgat ttttctacaa
     7441 tttcacccaa tttttcaatt ctcttaaggc ttgaaaaatc tatacaacaa attatgtctg
     7501 attcattaac atatttaata cccaattccg gatctttctc aaaattgata acagtttcat
     7561 tgcccttcat ccaaatcagg aaatcgggat aatcagtcgg agccagaaca tgtgtctggt
     7621 gtcctttttt gataaaataa ttactcaaac ccaaagcaga ccccagggca tccgcatctg
     7681 gtttctgatg gggaatgata gcaatcttct ttggggagga taatagatct ttgaattccc
     7741 tgatattgtg cattaagcgt tttccggtag atccaatatg cttataaaaa ggcctcaaag
     7801 ttgagataaa attcaaacaa atcaaacctg caagccaacc attaagctta aatgcgttta
     7861 ttcaaaaata ttattcttac ttttgcgatt ctaaaaaagg actaataatg agcggaaaca
     7921 gaacttttac tatgataaaa cctgatgcag taagtgccgg aaattcgggt gcaattttaa
     7981 agatgattga agaaacaggt tttaagatta ttgcgatgaa atatacgaag ctcactaaag
     8041 aaagagcagg tgaattttat gctattcata aagaaagacc attttatact gatttatgta
     8101 attacatgtc atcgggtcca ataattccca tcatattaga aaagtcgaat gcagtagaag
     8161 atttcagaaa actaatagga gccactaacc ctaaagaggc tgctgctggt actattagaa
     8221 atcttttcgc ggaatccatt gaagcaaatg ctgtccatgg ttcggattca gatgaaaatg
     8281 cggagataga agctgatttc ttttttagta aaaccgagcg gttccaggaa aattaattgg
     8341 accggactgc ttcaaaactt tcaatccaaa aattattaaa gccaattccg atatgcatgg
     8401 tgattagctt aagctgaagt aagtcaagaa ttgaaaggaa gttgaaaata acagcaatct
     8461 tggtaggatt gtcttcaata atttgggaga aagaaaccct ggattccttg ccaacctttt
     8521 gcagtatgta tttcttttgc tgttcaaggg tatatggcca ctgaataacc ttatgagatg
     8581 gtctattcag ttcatattca tagcgctcca ttaccctttg aaacactttc agaagcttat
     8641 aaagatcaag gttcagaagt tcccactcca cattagcatt ttctgacaga ctttttaatt
     8701 ccctggtaat atttcccctg ctctcaagtt gtgatctgtc ttcttcaaac ttagccagtt
     8761 ctgaaagcac agacttatat ttcttatatt ccaaaagatg cttcactaat tcctcccttg
     8821 gatctatttc ctttccttct tcatcaagta caggtctggg cagcagcatt ttcgctttta
     8881 tgcgcattaa ggttgctgcc accaaaatga attcgctggc aacctccata ttcatggttt
     8941 ccaaatggcg gatataatca aggaagtctt tcgttatctg tgatattgga atatcataaa
     9001 tatccaattc atccctttca ataaagaaca acaaaaggtc aaaagggcct tcaaaaaggg
     9061 gtaattttat ttcgtagctc aaattaaaag gttttcacaa aagtaaaatt ttggcccgga
     9121 taattcacca aacagggtta aaaatgtaaa tttgtgtcct ttattaatca agagtccaga
     9181 tttacgtaac aattgcctta tattgaacta atcatcccag aagcagatgt tgattaagcc
     9241 gggtcccctt cttgcgcaaa taaattcccc tgatgatgtc aagaaactgg acgctattca
     9301 attggttcag ctttgtgagg agttaaggca atttattatt gataatgtct cggtgtatgg
     9361 cggccatttc ggagcaagtt tgggggttgt tgagttaacg gttgccctcc attttgttta
     9421 tgacactcca aaggatcagc ttgtctggga tgttggtcac caggcgtatg gacataaaat
     9481 tctaaccggc cgaagagaaa atttcagcac caatagaatt tttaaaggtc tatcaggttt
     9541 tccaaaaaga aaggaaagtg aatatgatac tttcggtgta ggtcattcct caacttccat
     9601 ttcggccgcc cttggaatgg cagttgggaa taagtataag catgatcccc ggcatcacat
     9661 tgcggttatc ggggatgggg cccttacagg tggaatcgcc tttgaagcga tgaatcatgc
     9721 aggtgtttca aacacagacc ttcttattat tctcaacgac aactgcatg
//
LOCUS       DASWHX010000135        29061 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_1228165, whole genome shotgun sequence.
ACCESSION   DASWHX010000135 DASWHX010000000
VERSION     DASWHX010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 29061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29061
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1228165"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(278..1765)
                     /locus_tag="VGA21_09205"
     CDS             complement(278..1765)
                     /locus_tag="VGA21_09205"
                     /inference="COORDINATES: protein motif:HMM:NF024181.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HEX9650727.1"
                     /translation="MKTIKYFFTIVLLLNFLACDNVDFGDLNENKNGASEPYTAGLLS
                     NAIMAYATTTGRDGLMKPTLYVQYQAQVTYVDEMLYAETPSSWFTYYISSLQNLQAVI
                     DFISVPENQTPATLASGSVNNQVGVATIMKAIILKRVTDTYGDVPYSEALLGAGNIAP
                     AYDLQENIYKGLITELKTARDMLNATETGPIGDIIYYGDVNSWKKLANSVIMQMSLQL
                     SNRYPTPGGYAATEFAAALNDPNGVISTLADEAWFKFQNAPGFRNPWNANRTPDYFMT
                     REFSDALKGNATALNPTSNTTFDERINVFADDPTRDGVPYGKADGSGAGAASVSNTYY
                     WNNTTPLPLMTASYVFLNRADAAEMGWTGENVTNMLTSGITMSYRTLEALTENTGSPL
                     TLDETAYLAARLNDAANIGNAQVIAEEKWVSLFGQAFEAWAEWRRTDIPNLVPATDYF
                     NNGQIPRRYLYPIEENTLNGSKYDEGVSRLTPAEDKGTSRVWWDN"
     gene            complement(1777..5046)
                     /locus_tag="VGA21_09210"
     CDS             complement(1777..5046)
                     /locus_tag="VGA21_09210"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HEX9650728.1"
                     /translation="MKRRLLIFLAILYGISWSYEGFSQERTVSGKVTSGEDGSSLPGV
                     NILLKGTSTGTITDVDGAYRMTISGDNPVLIFSYVGYVNMEVEVGTRSIVEVTMESDV
                     TELSEIVVTALGVSRETKTLPYASQQVTQENLNITQDIDIRGALSGKIAGVQLNGQAG
                     SKLGQFGKIRVRGAISLTKDEDPLFIVDGVPTPDPNDIDMNNIESINVLKGPNATALY
                     GQRAEFGVIVITTKKGHEAEGLLVDYQMSTTWDKIGYLPKYQNLYGGGYEGEASWATF
                     DYGAGAFSAFPSAWSVFDGKRYITYDNNYADESWGPKFDGQDYVPWYAWWPDSPYYGQ
                     TAKYEAQPDNVKDFYETGVTAKNTISLSGSGSGYAARVSYSNVEQSGITPFTHYNKDF
                     LLVNTEFDPVNKLKISAGIRYSSSKTKGDFGDGYGNQTSGSFNSWFNRQLETDKLREL
                     KDLKTFDGYSTSWNWWGPEYYTLGGGYKKAAFWFNPYTFMEQYKQTRVNENFAGNFNV
                     AYKIIEDLEVSIGAGRNASEYKLDWYFPFFLANSSAPELYNSWSNSFGKYRRAVSENN
                     YHADLRYNKKFDVFDLAVLVGGNIRRDSYRNLSAQMPVGAKTGGLIIPDVYTFSNAGL
                     NVVPSYFEYKKQVNSFYGNLSAGYNGIIYIDASYRRDFSSALTESKNGYGYPAIGTSF
                     IFSELLGGFNALTFGKLRASWAQVGSDVDALALSPIYNIGAEPFVSNNTSFILMSNPT
                     QLVDPNIKPSLNTSFEVGFDTRFIQNRMGLSFTYYDETRKDEIIPVNIPRTSGYNTYL
                     TNAGESRRSGVEISLDADVIEMSNGITWNILFNFAKNKTTVESLPGDLEAINGVGGTG
                     TFGFISVINELGNNWGQLRGTAIERDANGNAIIQPSGLYKTIPDQYFGSILPDFTGGF
                     INRISYKGVELIASIDYQKGGKFFSLSEQWGAYSGLTEETAALNDLGMNVRDDVGDGG
                     GVRVVGVDANGNPVDMYVDGLSYYTQWYSNRLAEPFVHDASYIKLREVGIMFDVSKFI
                     NTGFIKAASLGFVARNVALLSVAKDNVHRWDPSILSTNYGENGQLPGTRSYGINLQIT
                     F"
     gene            5353..5712
                     /locus_tag="VGA21_09215"
     CDS             5353..5712
                     /locus_tag="VGA21_09215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650729.1"
                     /translation="MSGLTVPSKIIGFGIILIAGFLVSFLSDDVPQYLVKKLNIYTGN
                     FPQEKLYIQTDRAQYFAGDDIWLKIYLSNAETQKPDTLQSLLYLALVNDNQEIIANRR
                     VKMSKGIGESNFETDPC"
     gene            5875..6201
                     /locus_tag="VGA21_09220"
     CDS             5875..6201
                     /locus_tag="VGA21_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009583157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEX9650730.1"
                     /translation="MKKIQLGEFEELVLLTAGLLQEEAYGISISDELRKRTGRNPSIG
                     ALHTALNRLENKGFLKSKLEGATENRRGRRKRFYQITSKGAQALRHVYELRTDMIKLI
                     PNLANR"
     gene            6198..8837
                     /locus_tag="VGA21_09225"
     CDS             6198..8837
                     /locus_tag="VGA21_09225"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX9650731.1"
                     /translation="MKSNPPRLAEKFFRWFCHHNHLEGLEGDLYENFDHRVKENGLFK
                     ARLFYLVDIMTLIRSSVSKPLPRISTNNIMEIFFSNLKVAFRQGIKRKEFSLINIIGL
                     TLGITSILFITLFIKDEISYDSHITNAENKYRIYNIRRGTDGIDNYLPIVPPVFAAAF
                     QENFEQIEKIGRIINDYGGTIFTIGENSFSETKGFFSEIAALEILDLKVIHGDINNLS
                     ERYSVLLSQKTFEKFFGNETFGQQVVLIGSRELKVAGVFENLPVQSHLGVDYIFPFDY
                     AVSSVTPQRMSSWIWQQFFTYVQLSPGTNVEEFTNQVRNFVTEKTRDIGEQRFYYITH
                     FQNIRNIHLHSSNFEWDIAVIGNYQSIVFLAIAAGIILLIAVLNFINLTIAQALKRAK
                     EVSVRKFVGASKSQLFAQYSMESIVYAILAGIISVALLSILLPSFNDFTGKNLTYPDI
                     FTPNNISLYLALLVIIGLLAGIQPAFLLTSFKPLDAVRGITRISIGANAKIQVRQWLV
                     GAQYILSIGLIILSLIIQDQFSFLQNSDMGFDKENLVVMPLTNSLRNDLESTRAAFTD
                     YSGITNLSYCYGVPGGIVSGDGVVIPNKGEGEHGANVFIVDEGYISTMKMRIIAGRDF
                     SKDVTSDRSEGFVVNETAVKNFGLESPEKAIGEPLHWNIWGEDSIKRGAIIGVVEDFN
                     YKSLHNEISNTVLHIGPQYFSYMVLRLSPGDLSATLSNMEKAYRKFEPTRPFDYQFVD
                     QSFDQFYKSEEKLSQLFSIFTSLAIVTAAIGLFGLVSFSVVNRAREISIRKVLGAATT
                     TIFIMLVQRYFIMIALCLLIAVPLSWYIAREWLTNFAYHTNIEALVFVEVGFIIFVLT
                     LVTVGYHAIKGASANPSEKLRTD"
     gene            complement(8879..10243)
                     /locus_tag="VGA21_09230"
     CDS             complement(8879..10243)
                     /locus_tag="VGA21_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013455061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEX9650732.1"
                     /translation="MIKIATLCLFISIFFYSAALAQIPTLDTRHYDIINSVSAERLES
                     DIRKLVSFGTRHTLSDTVSQTRGIGAARRWIKSEFDEISKACGNCLEVVYQDYLVEGR
                     EQSRIKEDTHVISVLAIQRGTRYPNTYVIMSGDIDSRVSDAFNGKSDSPGANDNASGM
                     AGAIEAARVLSRYTFPASIIYVGLSGEEQGLFGGQYMAKNAKGQNWNIIGVLNNDMIG
                     NIEGVDGVIDNRSFRIFSEPTPVTETEEQRTRRRFYGGEVDGISRQLARYIHRMTITY
                     MPEMNPVMIYRLDRFGRGGHHRPFNDEGFAGVRIMESHENYNRQHQDLREENGIKYGD
                     VIEGVNFEYAAKLTAVNAIALAGLAWAPPQPGNVQIGGAVRPSTTLAWEGSDDPDLLG
                     YMIYWRETTGPQWQYSKFVGKVTEFTLEGIVIDNYLFGVSAVGKNGNESVVSFPGGLI
                     RRRD"
     gene            10296..11021
                     /locus_tag="VGA21_09235"
     CDS             10296..11021
                     /locus_tag="VGA21_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008198401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HEX9650733.1"
                     /translation="MKSIPRAVRKELKNRSNPKKREFYPKFFKAYKGGYGEGDKFLGT
                     IVPETRKVATLFWKGISIPDLVTILNDPYHEVRLCGLFILEKNYKKVKEENEKKKWVS
                     VYLDNLDLVNNWDLVDLSASHILGDYLKDKNRRILYELAKVDNLWRNRIAMISTQAFI
                     RNDDFTETLKLAEHFVGHPHDLMHKAVGWMLREVGSRNLMVERDFLDRFYKIMPRTML
                     RYAIEKFPEKLRKKYLEGKIQQK"
     gene            complement(11026..11451)
                     /locus_tag="VGA21_09240"
     CDS             complement(11026..11451)
                     /locus_tag="VGA21_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009581576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HEX9650734.1"
                     /translation="MSQENNFIIKNAPLSIEEVYNESLDQNSGAVNLFVGTVRNENNS
                     KKVAKLVYESYDSMVLAEFDKIARNAKSKWSVNKIVIHHRKGVLHPGDIAVIIAVSSP
                     HRQESFEACSFVIESLKKSAPIWKKEIYDDGQEWLESHP"
     gene            complement(11453..11692)
                     /gene="moaD"
                     /locus_tag="VGA21_09245"
     CDS             complement(11453..11692)
                     /gene="moaD"
                     /locus_tag="VGA21_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009581577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin converting factor subunit 1"
                     /protein_id="HEX9650735.1"
                     /translation="MKIKVLLFGIAREIAGSSSVELELENPKVDNLIKKLQDKHEEFR
                     QLKSVLVAVNNEYANPDQLLSSEDEIAVIPPVAGG"
     gene            11876..12334
                     /locus_tag="VGA21_09250"
     CDS             11876..12334
                     /locus_tag="VGA21_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008626161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX9650736.1"
                     /translation="MATYNIRVNGKSHHVEADADTPVLWVLRDNLGLVGTKYGCGIAQ
                     CGSCTIHLNGEAIRSCSTQVSQVADNEIITIEGLSKNGTHALQNAWREHDVPQCGYCQ
                     AGQIMNAAVLLNKNPNPSDAEIDEAMNGNLCRCATYVKIKAAIKTAAQNM"
     gene            12358..14496
                     /locus_tag="VGA21_09255"
     CDS             12358..14496
                     /locus_tag="VGA21_09255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020530330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX9650737.1"
                     /translation="MSLLKIKQDRRSFIKASVLTGGGIMLGFNWTFTGCTPAETVKAI
                     PSEWFKINAFLKIAANGMVTIMSPNPEIGQNVKTSMPMIIAEELDVNWDDVMVEQAPL
                     DTENYTRQVAGGSQSIRQGWTSLRTAGATARKMLINAAANQWEVDPSTLTTEAGFIHN
                     SNGNKLGYGEVATAAASLEVPVEVELKDPKDFKIIGTPRGNVDMEGIITGKSLFGLDY
                     KRDGMQYVVVLRPPAFGLKLKSFDDTAARQVNGVTNVVQFGNKIAVLGNNTWAAMKGK
                     RALNAEWEEDTKAESTAYHDETMRNHLNTKTEEPRRKDGNVDKVFKEGDAVIERVYEA
                     PFLPHNTMEPMNFFAHVTTEKVDLVGPIQTPEWTRSRVAELLGREQSEITIQMTRMGG
                     GFGRRLYGDFALEAAEISKETGLPIKVVFSREDDMTAGTYRPASKYKFKASIKESKMT
                     GYHLVGTGTNIGNATRQDNFPAAALEHYLCESHSLESNITTGAWRAPITNFLAFAEQS
                     FLDEVAEELRKDPIEFRLEMFERAKANPVGELNYEPDKFIGVTKLAREKSNWGNAGDG
                     VFQGFSAYYSHNTYVAEVADVVVENGRPKVKKMTVAIDCGIVVNPLGAVNQVQGGVID
                     GIGHALYGELTFVNGQPQSQNFNNFRLIRSSEAPLEVDVHFVKSYNDPTGLGEPALPP
                     AGGAVANAWYKATGQRLYKQPFMKTMIERS"
     gene            14564..15043
                     /locus_tag="VGA21_09260"
     CDS             14564..15043
                     /locus_tag="VGA21_09260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650738.1"
                     /translation="MKHLLTLSALLLMASCGQIQEKSNDGWDKEIVINEVTQMFDNYH
                     NDIEAEGLTAEFKYLDNSDDFFWVPPGYNSTLSYDSVRLILEKNAAAFTEIKFSWETL
                     IVVPLTDQIATYSGIVIGAMRDTAGVESPVRIIESGTVIKRNDGWKLLCGQSASLTQ"
     gene            15228..15554
                     /locus_tag="VGA21_09265"
     CDS             15228..15554
                     /locus_tag="VGA21_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015814845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX9650739.1"
                     /translation="MEIRRDVFQAIADPTRRSILSMLAAKPKNLNAIAEQFEMTRQAV
                     SLHVKILAECGVIAINRNGRERLCNLRPQKLTEVADWLYPFIQMWDDRFNQLDNVLGK
                     LKHKKK"
     gene            15551..16048
                     /locus_tag="VGA21_09270"
     CDS             15551..16048
                     /locus_tag="VGA21_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015813721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEX9650740.1"
                     /translation="MMKTIITKDLRNKRLNILREFDAPLELVWKAWTDSSILDQWWAP
                     KPWKAATKVMDFKEGGHWHYCMNGPDGEQHWARVDYEKIVPQENFSGMDSFCDEKGNL
                     NHDLPRMQWQVKFSGTGSTTRVEVEVTFASVEDLEKIVEMGFQEGFAAAHENLDELLK
                     ELVSI"
     gene            16253..16420
                     /locus_tag="VGA21_09275"
     CDS             16253..16420
                     /locus_tag="VGA21_09275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650741.1"
                     /translation="MKKIWILIHVHKGFIQEPEIFSDKKSAESRNRIIPNNFNRDYDD
                     EIGIHEKEVLE"
     gene            complement(16721..17377)
                     /locus_tag="VGA21_09280"
     CDS             complement(16721..17377)
                     /locus_tag="VGA21_09280"
                     /inference="COORDINATES: protein motif:HMM:NF016840.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX9650742.1"
                     /translation="MLFKAISLKLLVGITMLGYSCSETGDSYKPEWTDKDRKVLLDGL
                     SQSLDSLLILTGGISEEKLNQKPDSNSWSVAEVVEHLVLQDQAFYRELSAINSRPEEI
                     TLIENLESNDAEFLEYATDPNPGQADWFAVPTGNYKDFEVVLNHFITVRNETIKFITE
                     TKSDLRKHYTYREIPEHLLQNEFYRIRKVRDLHQLMLNEIAHTYRHLGQINRIRERVE
                     "
     gene            17471..18319
                     /locus_tag="VGA21_09285"
     CDS             17471..18319
                     /locus_tag="VGA21_09285"
                     /inference="COORDINATES: protein motif:HMM:NF039905.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEX9650743.1"
                     /translation="MRILIIHNPIAGNGKRTNVLEPFERKGFIIIETKGPNDATQIVH
                     ENIAGYDLFIAAGGDGTVNEIAKALIGTEKSMGIIPMGSGNGLVNEVGQITVKALLEN
                     NYRTTIIDSLFLNEIPFFNVAGIGFEAIVAHEFAKQKKRGLIGYFTTTISGIFSYKPL
                     IISYSFNDILKSDKIFSITIANSKQYGNNAVISPSSKPNDGIFEVCMIRSFPLLYGPI
                     LSYRLFSRTISKFKYYEVIMTTSFHLMNEISTLWNLDGESIRLPGPFHISIKPASLKI
                     ISEIPN"
     gene            18443..18976
                     /locus_tag="VGA21_09290"
     CDS             18443..18976
                     /locus_tag="VGA21_09290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650744.1"
                     /translation="MRKLLAIVFVSFFGFQTAGFLIVFELQKYCIRKEIKNMIKQGLP
                     EEDLVVFRIGEEEQKAFLKNFLFFDKNEFRFEGKMYDVVRVEMEANFIYYYCVSDEKE
                     SLLFANLDSSVRKSLDDSPSNQNQLRNFNTFISSLYYPQGNNIIFKNFSIGKNMSHYF
                     FSVQSWKNSPSTRPPKS"
     gene            19045..21450
                     /locus_tag="VGA21_09295"
     CDS             19045..21450
                     /locus_tag="VGA21_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013454966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX9650745.1"
                     /translation="MNNIFVFCLCFLVQQSFAQTLTIRDADTRHPIEMVTLYSSKPVV
                     SAFTDAKGNVDLSQFVDADTIYFRLIGYKPVQKTLPEIQRMNYILLMNPTNFSLQEIT
                     ISVSRWEQEKHDLPNKISSIKPEEIYLQNPQTAADILTISNEVYIQKSQLGGGSPMIR
                     GFATNRVLLVVDGVRMNNAIFRSGNLQNVISLDPFTIQSSEIVFGPGSVIYGSDAIGG
                     VMSFYTLSPSFSSTEKPLVKGSVFTRYSSANSELTGHLDLNVGFKKWGSSTSISYSNF
                     ENLKMGKHGPDEYLRPTYAVRINGVDTMLINSDPEIQIPTGYNQVNLMQKLRFVPTHN
                     WKIDYGFHYSTTSDFPRYDRHILYRNGLQRSAEWYYGSQTWMMNSLGILNSSETSFYN
                     QVKLTLSYQHFEESRHDRDFGDVIRYNRYEEVDAFGANLDFEKKISKKHSIFYGVEGI
                     LNEVGSTGIDTNIATQSESPGPTRYPDGSTWQSYAGYINYLVRTSKLLTIQTGLRYNH
                     ILLKSQFDTAFYPFPFSEANINAGAVTGSAGLAWSPLSSWQFNANFSSGFRSPNVDDV
                     GKVFDSEPGSVILPNPDLKPEYAYNFELGITHIFGDLAKLDATGYYTILKDAMVRRNY
                     RLNGQDSILYDGELSQVLSIQNSAKAFVYGIQTGVEVKLPAGFSLTSNINYQAGEEEL
                     DDESTAPLRHAAPLFGSTHILWTRGKLKADLYSFYNGRIAFKNLAPEEQNKSYLYAID
                     GNGNPYSPAWYTLNFKFLYHATNFITLNLGLENITNQRYRPYSSGIAAPGTNFIISTR
                     FTF"
     gene            21536..22312
                     /locus_tag="VGA21_09300"
     CDS             21536..22312
                     /locus_tag="VGA21_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017732495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX9650746.1"
                     /translation="MKKLSILIPVFNEERTIFNVLQKVTSVSLINDISKEIVIVDDYS
                     TDNTRSEIKKFIHDFPEMEIIFLEHQKNRGKGAAIRTGIERATGDYIIIQDADLEYDP
                     NEINRLLQPIVEENADVVLGSRFLSGSSKRVLYFWHSLGNKFLTLLSNFMTDLNLSDM
                     EVGYKLIPTSIFRYIKIRENRFGFEPEVVGKLAAIKGIRIYEVGISYHGRTYEEGKKI
                     NWKDGFRAIYCIFRYAPVFNRRLKNYKKDPQSAFQTGSKT"
     gene            22450..22818
                     /locus_tag="VGA21_09305"
     CDS             22450..22818
                     /locus_tag="VGA21_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015812445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HEX9650747.1"
                     /translation="MKASLTILLLIISNSFMTLAWYGHLKFYEMKGWEKLSLFSVILI
                     SWGIAFFEYCFQVPANRIGFQSQGGPFTLVQLKVIQEVITLVVFSLFTIMFFKTETIR
                     LNHFIGFIFLILAVYFIFKK"
     gene            23121..24143
                     /locus_tag="VGA21_09310"
     CDS             23121..24143
                     /locus_tag="VGA21_09310"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX9650748.1"
                     /translation="MIENEMKSDKVAALAVAVIDSGKVVHLNTYGFRDLENKFKATVN
                     TPFHIASVSKTVTNLAVFNLVESGKIELETDINAYLPFKVKNPHFPNTKVTVRDLLNH
                     RSGIRDDFEIYEPFWTIPNGDPTLELNDFLKNYLSAGGKLYKDGHFESNSNYKSFSYS
                     NTGVALLGLIVETVSGMKYEDYCQENIFKPIGMMNTSWFLKNLDSNLVAKTYVHEDSI
                     GLIFKGHNGYPDYPGGQLRTSISDYSLLLTGYLNAESSNFILDANTTRKITPLPQISH
                     EGFYTWFLNAINDNIYYAHPGGDTGVRTIVIIDVRNKNGIALFSNAEIELTNLLREIE
                     KKMWSK"
     gene            24203..25090
                     /locus_tag="VGA21_09315"
     CDS             24203..25090
                     /locus_tag="VGA21_09315"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX9650749.1"
                     /translation="MKDIVIETEDRFGLKATIYKASKNSIKNCTLIINSATGIGRKFY
                     SRYSKYMASNGFDVITYDYRGIAESRPQKLKGFKADFVIWGERDFSSVIKKSKNLLPI
                     NKIVVLGHSIGGTIFGMTLDCQEIEAVIMIGAQTSYYKDWPKKHRVKLYLLWHMVMPF
                     LTNLVGYFPGKKLRMMEDIPKGVVRQWNERRKNQSMAAQLQENNVNLFYHIYTKKLLT
                     IFIEDDVIGTKRAVERITELFICADRQVISVSPSDYSASVIGHFGFFKKDFEKNLWLQ
                     TVNWYTEAITTASSKFLPA"
     gene            25270..25620
                     /locus_tag="VGA21_09320"
     CDS             25270..25620
                     /locus_tag="VGA21_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009057240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEX9650750.1"
                     /translation="MTKFLRETYVYQNHFWDFYNNQKKEVQDKIDYVIGLVRTLQMVP
                     ERFFKHLEGTDGIYEMRVKVGSDIYRIFCFFDQGQLIILLNGFQKKKDKTPRQEIIRA
                     ERLKKEYYENKIKR"
     gene            25598..25909
                     /locus_tag="VGA21_09325"
     CDS             25598..25909
                     /locus_tag="VGA21_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009583609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX9650751.1"
                     /translation="MKTKLKDKYLTSWDDHLDKKYGKKGSKSRQKYEEDFETFKIGIL
                     IQEARKRQHMTQQELANKVGTTKNYISRIENNASDIRLTTLMRIIREGLGGRLKFSVD
                     I"
     gene            26166..26486
                     /locus_tag="VGA21_09330"
     CDS             26166..26486
                     /locus_tag="VGA21_09330"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX9650752.1"
                     /translation="MVDKKIFELHADVCKALGHPLRIEVIDLLQDKELCFTDILEVTG
                     GLKSNLSQNLSVMTKKGILKSRRDGQCTYFSLSSKKVAQACRLMRGVLIDSLKKQKNL
                     LEKM"
     gene            26498..27637
                     /locus_tag="VGA21_09335"
     CDS             26498..27637
                     /locus_tag="VGA21_09335"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HEX9650753.1"
                     /translation="MGKTILVLGAGTGGIITAKELSKKVGGKTKILLFEKEEKNIFAP
                     SLLWLMVGKRKPKQVYRNTRKITGAGIEVIIGEIEKVNPEKISVTVKGQEYEGDYMII
                     SLGVEQIAEHNLNDYGHDFYTLDGAAKFNEQLQNFKGGKIAVLVPSIPFKCPAAPYEA
                     AMLVENFIRKKELNSKAEVSLYVPEPGPMGVAGKELSGAVRQMVEMKGIKYFPEHQLT
                     SATERILTFNNGKTFEYDLMAFTPKHQCPSVIRETALVGKSGWIEVNRNTMETNFPNV
                     YAIGDITFIPLEIGKPLPKAGVFAHYQAETVAHNIAQKIAGKSSGKTFNGDGQCFLEM
                     GGGNAGYAGGNFYASPVPDVKMKKPGYFWHWTKVWFEKYWFFKYF"
     gene            27712..28065
                     /locus_tag="VGA21_09340"
     CDS             27712..28065
                     /locus_tag="VGA21_09340"
                     /inference="COORDINATES: protein motif:HMM:NF014672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HEX9650754.1"
                     /translation="MKILILINDAPYGTEKAYNALRLANQLNKDHETVEVRVFLMADA
                     ASCAVANQTTPNGYYNIERMLKLSLNKGTKVKICGSCADARGLKNVQLIEGTEISTMA
                     ELTNWVVDSDKVLIF"
     gene            28616..>29061
                     /locus_tag="VGA21_09345"
     CDS             28616..>29061
                     /locus_tag="VGA21_09345"
                     /inference="COORDINATES: protein motif:HMM:NF014177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0032775 - DNA methylation on adenine
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HEX9650755.1"
                     /translation="MATEIKYKIKAENVSTVQNLFNPCNVGARPFLKWAGGKGQLLDK
                     FQELYPKQLKQKKIKNFYEPFLGSGAVFFDIAQHYEIEGAYLYDINDELILTYKAIQK
                     DVTKLLDFLYRYQKKYIKLDKKQRQEFFYDQRTNYNLQRFNIDYDK"
BASE COUNT         9055 a         5698 c         5798 g         8510 t
ORIGIN      
        1 cagggggttt gaataccatt ttaagaacca cccctagccc ctccttaaat taaggagggg
       61 aaagtacccc ctgcccagaa aaattgtccc ccttgtttta agggggacta cagggggtaa
      121 ttaccttata tagcaaccgc atttctattt cacaaccacc cttgcctcct aaaaaaggag
      181 gtgattatac ttagacaagt ttttcattgg aaggataaca aaaaaggcta tctgatagat
      241 agcctttttt aagacatgtt tcaattaatc caaataccta attatcccac caaactctgg
      301 aagttccttt gtcctctgcc ggagttaatc gtgaaactcc ttcatcgtat ttggaaccat
      361 tcaaagtgtt ttcttcaatt gggtataaat accttcttgg tatttgtcca ttgttaaaat
      421 aatctgtggc cggaaccaga ttagggatat ccgttcttct ccattcggcc catgcctcaa
      481 atgcctgacc gaacaaactc acccattttt cttcagcaat tacctgggca ttaccaatgt
      541 tggccgcgtc attgagtctg gcagctaaat aggccgtttc atccaatgtc agagggctgc
      601 cggtattctc tgttaaagct tccagcgtcc gataagacat ggtaatacct gaagtaagca
      661 tatttgtaac attttctcca gtccaaccca tttctgcggc atccgctcta tttaagaaaa
      721 cataggaagc tgtcatcaga ggaagtggag tagtgttgtt ccaataataa gtatttgaaa
      781 ctgaagcagc gccggcacca cttccatctg ccttaccata aggcacacca tccctggtgg
      841 gatcgtccgc aaacacatta atacgttcat caaaagttgt atttgaagtc ggatttagag
      901 ccgtggcatt tcctttcaat gcatcagaaa attccctggt catgaagtag tccggggtcc
      961 tgttggcgtt ccagggattc ctgaaaccag gtgcattctg aaacttgaac catgcttcat
     1021 cagccagagt gctaatgaca ccattgggat cattcagggc tgcggcaaat tcggtagcgg
     1081 catatccacc gggggttgga tatcgatttg ataactgaag agacatttgc atgattacgg
     1141 aatttgcaag tttcttccag ctattaacat ctccgtaata aatgatatcc cctatcggtc
     1201 ctgtttccgt agcattcagc atatcacggg cggtttttag ttctgtgatc agccctttgt
     1261 agatgttttc ctgcaaatca taggccggtg ctatattgcc tgctcccagt aatgcctcag
     1321 aatacggaac gtcaccgtag gtatcggtca cccgcttaag aatgatcgcc ttcattatgg
     1381 tggctacgcc tacctgattg ttaaccgagc cgctagcaag cgtggctggg gtttgatttt
     1441 ccggaacaga aatgaaatca attactgctt gcagattctg caacgaactt atgtaatagg
     1501 taaaccagga acttggagtc tctgcgtata gcatttcatc cacgtaggtt acctgagcct
     1561 ggtattgaac gtacaaggtt ggtttcatta atccatccct tccggtagta gtagcatacg
     1621 ccattattgc atttgataat aacccggcag tataaggttc tgaagctccg tttttatttt
     1681 cattcagatc tccaaaatcc acattatcac atgccaggaa attaagtagt agaacaattg
     1741 tgaaaaaata tttaatagtt ttcatggctt cttaatttag aaagtaattt gaagatttat
     1801 tccataactc cgggttccgg gtaactgtcc attttcacca taattggtgg aaaggattga
     1861 tggatcccat ctatgaacat tgtctttagc aaccgatagc aatgccacat ttcttgcaac
     1921 aaagcctaga gaagcagcct ttataaatcc agtgttaata aactttgaga catcaaacat
     1981 gattcccact tcccgtaatt tgatgtagct ggcatcatga acaaaaggtt cagcaagtct
     2041 gttgctatac cattgagtat aataacttaa accatctaca tacatgtcca ccgggtttcc
     2101 attggcatca acgccgacaa cgcgaactcc accaccatct ccaacatcat cccggacatt
     2161 catacccaga tcattaagtg ccgcggtttc ttcggtaagt cctgaataag caccccattg
     2221 ttcagataaa gaaaagaact ttcctccttt ttggtaatct atggatgcaa taagctcgac
     2281 tcctttatat gaaattctgt tgatgaatcc acctgtgaaa tcaggtagaa tggaaccaaa
     2341 atattgatca ggaatagttt tatacaaacc gcttggttga atgattgcat ttccatttgc
     2401 atccctctca attgcggttc ctcttagctg accccagtta tttccgagtt cattaatgac
     2461 tgaaatgaaa ccaaatgtac ctgttccacc aactccgtta attgcttcca aatctcctgg
     2521 aagagattca acagtagttt tatttttggc gaagttgaat aagatgttcc aggttattcc
     2581 attgctcatt tcaatgacgt cagcatcaag actaatctcc acacctgatc ttcttgactc
     2641 acctgcgttg gtcaaatacg tgttgtatcc tgaagtcctt gggatgttca ccggaattat
     2701 ttcatccttt ctggtttcat cataatacgt aaaactcagc cccattctgt tctggataaa
     2761 acgagtatca aaacccacct caaaggatgt gtttaatgat ggcttgatat tcggatcaac
     2821 cagctgggta ggatttgaca tcagaatgaa gctggtatta ttgcttacaa atggctcggc
     2881 acctatattg taaatcgggc ttaaagccag agcatctaca tctgaaccta cctgggccca
     2941 gctagctctc agttttccaa aagttagggc attaaagccg ccgaggagtt cagaaaaaat
     3001 gaaacttgta ccaatagcag ggtagccata gccattctta ctctcggtta gggctgagct
     3061 aaaatccctg cgataactgg catcaatata aatgatgccg ttatatccgg cagataaatt
     3121 tccataaaaa ctgtttactt gcttcttata ttcaaagtag ctaggaacta catttagacc
     3181 ggcgtttgaa aaagtataaa catcaggtat gattaatccc cctgttttgg ctcccacagg
     3241 catttgcgca ctaagattcc tgtagctatc tcttctaata ttgcctccaa caagaactgc
     3301 caaatcaaag acatcaaatt tcttgttgta tcttaaatca gcatggtaat tattctcaga
     3361 gaccgctctt ctatacttgc caaagctatt gctccaggag ttatataatt ccggagctga
     3421 tgagttggcc aggaaaaatg ggaaatacca gtcaagttta tactctgatg catttcgacc
     3481 tgcaccaatt gatacttcca gatcctctat gattttataa gcaacgttaa aattccccgc
     3541 gaagttctca ttaacccggg tttgcttata ctgttccata aaagtatagg gattgaacca
     3601 gaacgcagcc ttcttataac cgcctcccaa tgtataatat tcaggacccc accagttcca
     3661 tgatgtcgaa taaccatcaa aggttttcaa atctttcagc tctcttaact tgtctgtttc
     3721 aagttgcctg ttaaaccatg aattaaacga acctgaagtc tgattgccgt aaccatcacc
     3781 aaaatctccc ttggttttag aacttgaata cctgatacca gctgagattt ttagcttatt
     3841 tacaggatca aattcagtat ttaccaaaag gaaatctttg ttataatggg taaaaggtgt
     3901 aattccactc tgttccacat ttgaataaga caccctggcc gcataaccac ttcctgatcc
     3961 gcttaatgaa attgtgtttt tcgccgtgac ccctgtctca tagaagtcct ttacgttatc
     4021 tggctgcgcc tcatactttg cagtttgtcc ataataagga ctatcaggcc accacgcgta
     4081 ccacggtaca taatcttgtc catcaaattt aggaccccag ctttcatccg cataattatt
     4141 atcataggtg atatagcgtt tgccgtcaaa gacactccat gcagaaggga acgcactaaa
     4201 tgcgccagcc ccataatcaa aggtagccca ggatgcctcg ccttcatagc cgccaccata
     4261 caaattctgg tatttaggca gataaccaat cttgtcccag gttgtgctca tttggtaatc
     4321 aaccagcaga ccctcagctt cgtggccttt cttggttgta atcacgatca ctccaaattc
     4381 cgccctctgt ccatataaag cggttgcatt aggacccttc aacacgttga tggattcgat
     4441 gttgttcatg tcaatgtcat tgggatcagg tgttggaacc ccatccacaa taaacaaggg
     4501 gtcttcatcc tttgttaatg agattgcacc cctgactctg atcttcccaa attgaccaag
     4561 ttttgaacct gcctggccat tgagctgaac accagcaatc ttaccggata gtgctcccct
     4621 gatatcaata tcctgggtaa tgttcaaatt ttcctgggtg acttgttgag atgcataggg
     4681 aagagttttt gtttccctgc ttacacccag tgcagtaaca acaatttcgg ataactctgt
     4741 aacatccgac tccattgtaa cttcaacaat ggatcgagta ccaacttcaa cttccatatt
     4801 aacataaccc acgtagctga atatcagtac ggggttgtct ccggaaattg tcattcggta
     4861 agcgccatct acatccgtaa ttgtaccagt cgaagtacct tttaatagga tgttaactcc
     4921 tggtaaggag cttccgtctt ctccggaagt caccttaccg ctgactgttc gttcctgact
     4981 aaatccttca taagaccacg agatcccata aaggattgcc aggaagatta gtaatcttct
     5041 tttcatgatt tttactggtt agttgtttta ttaggttaac ctgctttagg ttaaaaatta
     5101 aggtagggaa ttaccatcct aaagtcaatg ataaataccc catattaggg gattttatta
     5161 ttggaaaatt tgattagaga tttgagtata aggattacat atatatatat gtagttctat
     5221 catgatttaa acaattgaat ccgagtaaaa tcataattta tcttattatt tccatcaata
     5281 tattaagggt tagcttatgg cgatccggaa gctgtattcc cgtagtaata aatgttaaat
     5341 tcatgtcagc atatgtccgg attaacggtt ccttcaaaaa ttattgggtt tggcataatc
     5401 ttgattgcgg gtttccttgt ctcctttttg agtgatgatg taccccaata cctggttaag
     5461 aagctgaata tatatacagg aaattttccc caggaaaaac tttatataca aacagatcgg
     5521 gctcaatatt ttgcaggaga cgatatctgg ctgaagattt atttaagtaa tgcagaaact
     5581 caaaagccgg atactttgca atctctgctc taccttgccc tggtgaatga caatcaggag
     5641 ataattgcta atcgcagggt aaagatgtcg aagggaattg gagaatctaa ttttgaaact
     5701 gatccttgtt gaaggattta cagaccatgg attccccgta agtggtactt atcactatac
     5761 cgtaaataaa taattctgaa ttttctgtaa cttttcgcat taaaaagagt ttataactac
     5821 aaataggaaa tattaattaa attacttcta cgtttaggaa aaataaatgt tcttatgaag
     5881 aaaatacagc tcggtgaatt tgaagaactg gtattactta ccgcaggact tttgcaggaa
     5941 gaagcgtatg gaataagtat ttccgatgaa ttaaggaaaa gaaccggtag aaacccaagt
     6001 attggagcct tacatactgc tcttaatcgt ctggaaaaca agggattttt gaaatcaaag
     6061 cttgagggag ctactgaaaa ccggcgtggg aggagaaaac gattttacca gataaccagt
     6121 aaaggtgcgc aggctctcag gcacgtgtat gaacttagga cggatatgat aaaactaatt
     6181 ccgaacctgg caaatagatg aagtcaaatc ctccacgcct ggcagaaaaa tttttcagat
     6241 ggttttgcca tcacaaccat cttgaaggac tggaaggcga tctttatgaa aactttgatc
     6301 atagagtaaa ggaaaacgga ttattcaaag cccggttatt ttatcttgtg gatataatga
     6361 cgctaatccg ttcttcagtt tcaaaaccat taccccgaat ttcaacaaat aatattatgg
     6421 aaatattttt tagcaatctg aaagtagctt ttcggcaggg aattaagcgt aaggaatttt
     6481 cgctgatcaa tatcattggc ttaacgctgg gaataaccag tattctattt attactcttt
     6541 tcatcaaaga tgaaattagt tacgacagcc acatcaccaa tgctgaaaac aagtatcgta
     6601 tctacaatat aaggcgcgga acagatggta ttgataacta tctgcccatt gtacctccgg
     6661 ttttcgccgc agcttttcag gagaactttg aacaaattga aaagattgga aggattatta
     6721 atgattatgg tggaaccatt tttacaattg gagaaaattc cttttcagaa accaaaggat
     6781 ttttcagtga aattgccgcg ctcgagattc tggatttaaa agtaattcac ggagatatta
     6841 acaatctaag cgagagatat tcggtattgc tttctcaaaa aacatttgaa aaattctttg
     6901 gtaatgaaac ctttggacaa caagtagtac taattggatc ccgggaattg aaagtagccg
     6961 gtgttttcga aaaccttcct gtccaatctc acctgggtgt ggattatatc tttccctttg
     7021 attacgccgt atccagcgtt actcctcaaa gaatgagcag ttggatttgg cagcagtttt
     7081 ttacatatgt tcaattaagc cccggaacga atgttgaaga gtttacgaat caggtcagga
     7141 attttgtaac cgaaaaaacc agagatatcg gggaacagag attttattac atcacgcatt
     7201 ttcaaaatat cagaaacatt catctccatt catcaaattt tgaatgggat atcgctgtaa
     7261 taggaaacta tcagtccatt gtttttcttg ctatagcggc gggcattatt ctgctgatag
     7321 cagttctaaa ttttatcaac ttaactatcg ctcaggcatt gaaacgtgcc aaggaagtga
     7381 gcgtgagaaa attcgtcgga gcaagcaaat cgcaactttt tgctcagtat tcaatggagt
     7441 ccattgttta cgccatcctt gccggaatta tcagtgtggc acttttatca attcttcttc
     7501 catcctttaa tgacttcacc ggaaaaaact taacataccc ggatattttt actccaaata
     7561 atattagtct ttacctggca ctgcttgtca ttatcgggtt attggcggga attcaacccg
     7621 cttttctact aacaagtttt aaaccacttg atgccgttag aggaattacc agaatttcaa
     7681 tcggagccaa tgcaaaaatt caagtaagac agtggttggt tggagctcag tatattttat
     7741 caatcgggct tataatttta tcgctcataa tacaagatca attttcattc ctgcagaaca
     7801 gcgatatggg ctttgataag gaaaacctgg tagtaatgcc actaacgaat agtctaagaa
     7861 atgatttgga atcaacccgg gctgctttca ccgattattc cggaattaca aatctaagct
     7921 attgctatgg tgttcccgga ggaattgttt caggtgatgg agtagtaata ccaaataaag
     7981 gagaaggtga acatggcgcc aatgttttca ttgtagatga aggttatatt tctactatga
     8041 aaatgagaat tattgccgga agggatttct ctaaagatgt aacaagcgac aggtctgagg
     8101 ggtttgtggt aaatgaaaca gcggttaaaa actttggatt ggaaagtcct gaaaaagcga
     8161 ttggagagcc gcttcactgg aatatttggg gcgaggattc cattaaacgg ggagcgataa
     8221 ttggtgttgt tgaagacttc aattataaaa gtctgcacaa tgaaatttcc aatactgttt
     8281 tgcatattgg tccacagtac tttagttaca tggttctcag attatcaccc ggagacttat
     8341 ctgcaacctt aagcaatatg gaaaaagctt accggaagtt tgaaccgacg cgtcccttcg
     8401 attatcagtt cgttgaccag agcttcgatc aattttataa atcagaagaa aaactatctc
     8461 agctatttag tatttttact agccttgcca tagttacggc agcaattggg ttattcggac
     8521 ttgtgagctt tagcgtggtg aatcgtgccc gggaaatcag catcagaaaa gttcttggtg
     8581 cagcaactac caccatcttt atcatgctcg ttcagcgtta tttcattatg attgcactgt
     8641 gcctgttaat cgcggtgccc ctttcctggt acatagccag ggaatggctc acgaattttg
     8701 cctatcacac caatattgag gcgcttgttt ttgtggaagt tggcttcatt attttcgtgt
     8761 taacgctggt tactgtaggg tatcatgcaa ttaaaggggc gtctgcaaat ccgtcagaga
     8821 aactgagaac agattaatga aagtcctgga aatccctgat tcagtaaata aaattctcct
     8881 aatctcttct tcgtatcaat ccacccggaa aagaaaccac gctttcattt ccatttttgc
     8941 cgactgccga aactccaaac aagtagttat caattacaat tccctccagt gtaaattccg
     9001 ttaccttacc cacaaatttt gaatattgcc actgcggccc ggtggtttct ctccagtaaa
     9061 tcatatatcc taaaaggtcc ggatcatctg atccttccca tgcgagtgtg gttgaaggcc
     9121 ggaccgcacc accaatctga acatttccag gttgtggcgg tgcccaggcc aaacccgcaa
     9181 gtgcaatggc attcacggcg gttaatttag cggcgtattc aaaattaacc ccctcaatta
     9241 catcaccata tttaattcca ttttcctctc gcaaatcctg gtgctgccgg ttatagtttt
     9301 catggctttc cattatccgg actccggcaa aaccttcatc attaaaggga cgatggtgac
     9361 caccccgacc aaacctatcc aacctgtaaa tcatgacagg gttcatttcc ggcatgtagg
     9421 ttattgtcat tctatgaata tacctcgcca gctgccggga aattccatcc acttcaccac
     9481 cataaaacct gcgccttgtc ctttgctctt cagtttcggt tacgggtgtc ggttcagaaa
     9541 aaatcctgaa cgatcggtta tcaattacac catctactcc ttcaatattc ccgatcatgt
     9601 cgttgttcaa tacgccgata atattccaat tctgtccttt ggcattcttt gccatatatt
     9661 gcccgccaaa taaaccctgc tcctcgcctg ataacccaac ataaattatg ctggcaggaa
     9721 atgtgtacct ggataaaact cttgccgctt ctatcgcgcc ggccattccg gaggcattgt
     9781 cattggctcc cggtgaatca gatttaccat taaaagcatc gcttactctg ctgtcaatat
     9841 ctccggacat gattacataa gtgtttggat accgggttcc tcgctgaatg gcaagcacac
     9901 tgattacatg agtatcctct ttaatgcgcg attgttcacg accttccacc aggtaatcct
     9961 ggtaaaccac ttcaaggcaa tttccacaag ctttggaaat ttcatcaaat tcgcttttta
    10021 tccacctccg cgcggcgcca attcccctgg tttgagaaac agtatccgat aaagtgtgcc
    10081 gggtgccgaa actcacaagt tttctaatgt cagattcaag gcgttcagct gaaacactat
    10141 taatgatatc ataatgccgg gtgtctagcg ttggtatctg ggccagagcc gcactgtaaa
    10201 aaaatattga aataaataga cagagggttg caatcttgat catagtcagg taatttattt
    10261 gccaggtgtt aaagttacta ttaattgaaa attaaatgaa gtctattcca cgtgcggtta
    10321 ggaaagagtt gaaaaatcgc tccaatccaa agaaaagaga attttatcca aagtttttta
    10381 aagcctataa aggaggttat ggcgaaggtg ataaattttt aggaacaatt gttccggaga
    10441 ccaggaaggt agcaaccttg ttttggaaag ggatcagtat tccggatttg gtaacgatcc
    10501 ttaatgatcc ttaccatgag gttagattat gcggattatt tattctggaa aaaaattata
    10561 agaaggtaaa ggaggaaaat gagaaaaaga aatgggtttc agtttacctt gacaatttgg
    10621 atttggtaaa taattgggac cttgtggatc tatcggcaag tcatatactc ggtgattatt
    10681 taaaagataa gaatagaaga atactttatg agctggcgaa agttgataat ctttggcgaa
    10741 accggattgc catgatatca acccaggcat ttattcgtaa tgatgacttc actgaaactt
    10801 taaagctggc tgaacatttt gttggccatc cacatgattt aatgcataaa gcggttgggt
    10861 ggatgttgcg ggaagttgga agccgaaacc tgatggttga gcgggatttt ctcgatcgct
    10921 tttataagat tatgcctcgc accatgttgc ggtatgcaat tgaaaaattt cctgagaagt
    10981 tgaggaaaaa atatttggaa ggaaaaatcc agcaaaaata atgggttacg gatgcgattc
    11041 aagccattcc tgaccatcat cgtaaatctc tttcttccaa atcggtgctg atttcttcaa
    11101 actttcaatc acaaaggagc atgcttcgaa agactcctgg cgatgaggcg aactgaccgc
    11161 aatgattaca gcaatatctc cagggtgcaa aacacccttt ctgtgatgga taacaatttt
    11221 atttacactc catttagact ttgcatttcg ggctatctta tcgaactctg caagaaccat
    11281 tgaatcgtat gactcatata ccaattttgc cacctttttg gagttgtttt catttcttac
    11341 tgtgccaaca aagagattta cagctcctga attttgatca agactttcat tgtatacttc
    11401 ctctattgaa agcggcgcgt tcttaataat aaaattattt tcctgagaca ttttaacctc
    11461 cagccacggg gggtattacg gcaatttcat cctcactgct gaggagctga tctggatttg
    11521 catattcatt attcaccgcc accaacacag acttcagttg cctgaattct tcatgtttat
    11581 cctgcagctt cttaatcaga ttatcaacct tcgggttctc caactcaagt tctacactgg
    11641 aacttccggc aatttcccgc gctattccga aaagcaatac ctttattttc atcagcgcta
    11701 atttagctaa tttttagttg aagatgattt ataaaatttt attaatccaa ttttacattc
    11761 ctttaatcaa atccactctc cattaaaata gattttaacc atgattgtat aaaggaatat
    11821 tgattagctg tttccttatt gctatattga atcacttaca tccaaatttt ttactatggc
    11881 tacatacaat attcgagtaa atggaaaatc gcatcacgtt gaagctgatg ccgacacacc
    11941 cgttctctgg gtgttacggg ataatctagg gctggttgga accaaatacg gctgcggaat
    12001 tgcgcaatgc ggctcttgca ctatccactt aaacggagaa gctattcgtt cctgttccac
    12061 ccaggtatcc caggtagccg ataacgagat tatcacaatc gaaggattgt caaaaaatgg
    12121 tactcacgcg ctgcaaaatg catggcgtga acatgatgta cctcaatgcg gatattgcca
    12181 ggcgggacaa atcatgaacg ccgctgtttt actaaataaa aatccgaatc cgtctgacgc
    12241 ggaaattgat gaggccatga acggaaatct ttgccgatgt gcaacttacg tgaaaataaa
    12301 agcggcaata aaaaccgccg ctcaaaatat gtaattctaa ctataactct gaaagaaatg
    12361 tcattactaa aaataaaaca agatcgcaga tcatttatca aagcatcagt attaacgggc
    12421 ggaggtatta tgctgggatt taactggacc ttcaccggtt gtacacctgc agaaactgtt
    12481 aaagctatcc ccagcgaatg gtttaaaatc aatgcattcc tgaagattgc cgcgaatggt
    12541 atggtaacta tcatgtcacc aaatccggaa attggccaga atgtgaaaac ttctatgccc
    12601 atgataattg ctgaagaact ggatgtgaac tgggatgatg tgatggttga acaagctcct
    12661 cttgataccg aaaattacac ccggcaggtt gccggaggta gtcaatccat ccggcagggc
    12721 tggacatctt tgcgaacagc cggagccaca gcccgcaaaa tgctaatcaa tgctgccgcg
    12781 aatcaatggg aggtggatcc gtcaacttta acaaccgaag cgggattcat tcacaattca
    12841 aatggaaata aattgggata tggagaagta gcaaccgccg ctgcatctct ggaagtgcct
    12901 gttgaagtag aattgaaaga tcccaaggat ttcaaaatta taggaactcc acgcggtaat
    12961 gttgatatgg aaggtataat tacgggtaaa tctctttttg gtcttgacta taagcgggat
    13021 ggaatgcagt atgtcgtagt tttacggcca cccgcctttg gtttaaaact gaaatcattt
    13081 gatgacaccg ccgcgaggca agttaacgga gttaccaatg tcgttcaatt cggtaataag
    13141 atcgccgtat taggtaataa tacttgggca gctatgaaag gaaagcgtgc gttgaacgcg
    13201 gaatgggaag aagatacaaa agcggaaagc actgcatacc acgatgaaac tatgcgcaat
    13261 cacctgaata caaagacaga agaacccaga agaaaagacg gtaatgtgga taaagtgttt
    13321 aaagaaggag acgctgtcat tgagcgggtt tatgaagccc cgttccttcc tcacaacacg
    13381 atggaaccga tgaacttctt cgcccatgtt acaacggaaa aagttgatct tgtcggacca
    13441 attcaaaccc ccgaatggac tcgctcacgg gttgcggaat tattgggcag ggaacaaagt
    13501 gagatcacca ttcagatgac tcgcatgggc ggtggatttg gtcgcagact ttatggtgat
    13561 ttcgcgttgg aagccgctga aatttcaaaa gaaacaggtt tacctattaa ggtcgtattt
    13621 tccagggaag atgatatgac tgccggaaca taccggcctg catcaaagta taagttcaaa
    13681 gcaagcatta aggaaagtaa gatgacggga tatcaccttg taggaacagg tacaaatatc
    13741 gggaatgcga cgcgtcagga taacttccct gccgctgccc tggagcatta cctctgtgaa
    13801 tcacatagcc tggaaagtaa tattacgacc ggggcatggc gcgcgccaat tactaatttt
    13861 cttgcatttg cagagcagag ctttctggat gaggtagccg aagaacttcg taaagatccg
    13921 atcgaattcc gtctggagat gttcgaaagg gcaaaagcaa acccggttgg agaattgaat
    13981 tacgaaccgg ataaatttat cggcgtaacc aaactggcca gggaaaaatc aaattggggc
    14041 aatgccgggg atggagtatt ccagggcttc agcgcttact actcccacaa tacctacgta
    14101 gccgaagtag ccgatgtagt agtagaaaac ggcagaccca aagtgaagaa aatgaccgtg
    14161 gcaattgact gcggtattgt tgtaaacccg cttggtgctg taaaccaggt tcagggagga
    14221 gtaatagatg gaatcggtca tgccttgtac ggcgagctga cttttgtgaa tggccagcca
    14281 caatcgcaga acttcaacaa cttcagatta atcagaagta gtgaagcacc tctggaggta
    14341 gatgtccatt tcgtgaagag ttataatgat ccaaccggac ttggcgaacc cgcgttaccg
    14401 cctgcgggtg gtgctgtagc caatgcctgg tacaaagcaa caggccagcg gctgtataag
    14461 caaccattta tgaaaactat gattgaaaga agctgagcag tgaaacagtg aaacagtgaa
    14521 acagtgaaac agtgaaacag tgaaacagtg aaacagtgaa acagtgaaac atttgttaac
    14581 tctgtccgct ttacttctga tggcttcctg tggtcagata caggaaaaaa gtaatgatgg
    14641 ttgggacaaa gaaatcgtta ttaatgaggt aactcaaatg ttcgacaatt atcacaatga
    14701 tatcgaagct gaagggttga cggctgagtt taaatatctg gacaactccg acgatttctt
    14761 ttgggttcct ccagggtata attctactct gtcttatgat tccgtgaggc ttattcttga
    14821 aaagaacgcg gcagcattta ctgaaataaa attctcgtgg gaaacattaa tcgtagttcc
    14881 acttactgat caaattgcga cttattcggg aatagtaatt ggcgctatga gagacactgc
    14941 aggagttgaa tctcccgtcc ggattatcga atcaggaaca gttattaaaa gaaatgatgg
    15001 ctggaaattg ctttgcggac aatcggccag ccttacccaa tgatatttaa taacacgcca
    15061 cggtggcatc catttaatgt ttcattctga accattcctt ttgcgcattt tttgacagct
    15121 ggattttgct tgatatgcgt tcattccact cttcatagta aaaatataac aattttcttg
    15181 cgcaagtaaa tacttgcata tatatttgca ggcatatact ttttaatatg gaaatccgaa
    15241 gagatgtatt ccaggcaatt gcagacccaa cgcgacgtag tattctttcc atgcttgcag
    15301 cgaaaccgaa aaacctgaat gctattgccg aacaatttga aatgaccagg caagctgttt
    15361 cacttcatgt aaaaatactt gccgaatgtg gagtcattgc tattaaccga aatgggcgtg
    15421 aacgtctttg taatcttcgg ccgcaaaaat taactgaagt ggcagactgg ttatacccgt
    15481 tcatccagat gtgggatgac cgcttcaacc aattagataa tgtactgggt aaactaaaac
    15541 ataagaaaaa atgatgaaaa caattattac caaggacctc cggaataaga gattaaatat
    15601 tttgcgagaa tttgatgcac cccttgaatt ggtctggaaa gcctggacag acagttccat
    15661 tcttgaccaa tggtgggctc ccaaaccctg gaaagctgct acaaaagtta tggattttaa
    15721 agaaggtgga cattggcact actgcatgaa tgggccggat ggcgagcagc actgggcacg
    15781 agttgactat gaaaagattg ttccccagga aaacttttca ggcatggatt ccttttgtga
    15841 tgaaaaagga aatttgaatc atgatttgcc ccgcatgcag tggcaggtga agttcagcgg
    15901 caccggctca acaaccaggg tggaagttga agtgacattc gcaagtgtag aagacttgga
    15961 aaaaattgtt gaaatgggct tccaggaagg ctttgcagct gctcatgaaa accttgatga
    16021 attactcaaa gagttagttt ctatttaaag ttacgtccga atcagaaact gtccagatac
    16081 tgttccggtc ctaagccata gggacagtat ctgcatttca tttcaggttc aaaattcatc
    16141 tccttgtcaa agtagaccga cttttaaaat gagtggtctt tctgaactct gcaatttcct
    16201 tgaatacaaa gatgtattgg gatggtttaa tagaactctt aaatcttaat gcatgaaaaa
    16261 aatatggatt ttaattcacg ttcacaaagg ttttattcag gaaccagaaa ttttttctga
    16321 taaaaaatca gcagaaagta gaaatcgaat aattcctaat aatttcaatc gtgattatga
    16381 tgatgagatt ggaattcatg agaaggaggt tctggaatag cacaaaaata ttcacatcta
    16441 atctttttgt aaatttgtag ttcatatata tgaactatgg caataggtag aattttacag
    16501 gtaaatgaat caacgataaa ggttatcact caaaacgaaa ctgacttcat cagtctgact
    16561 gatatgacag caggtttaag gaaggaagtg gcttaatcgg taaatggatt accaacaaaa
    16621 acacgttgga atatctgggc gtttggaaaa tatcaacaac cagaattgca attaccccga
    16681 attcgaggta attgcacgta aacgcaaaac gttattttca ttattctacc ctttctctaa
    16741 ttctattaat ttgcccaagg tgccgataag tgtgtgcgat ttcattgagc attaactgat
    16801 gcaagtcccg gaccttcctt attctgtaaa attcattttg caacaaatgt tcagggattt
    16861 cccggtaggt gtaatgtttt cgcaaatccg atttcgtttc tgtaataaac ttgattgttt
    16921 cattccgcac cgtgataaaa tgattcaaca caacttcgaa atccttataa ttgccggttg
    16981 gcacagcaaa ccaatctgcc tggccggggt ttggatcggt ggcatactcc aggaattcag
    17041 catcattgga ttccagattt tcaattaagg taatttcctc cggcctcgaa ttgattgcgc
    17101 tgagttcccg gtagaaagcc tgatcctgca gaaccaaatg ttcaaccact tcagccacgg
    17161 accacgaatt gctatccggt ttttgattta gtttctcctc agaaatgccg ccggttagaa
    17221 ttagaagact atccaacgat tgactcagac catcaagcaa aaccttccga tccttatctg
    17281 tccactcagg tttataggag tcacctgttt ctgaacatga ataacctaac attgtaattc
    17341 caacgaggag ctttaaagaa atagctttga ataacatgct ttaatttaac taattctgca
    17401 aagcttaatt taataacaat gaagattctg ttaacataaa ttccttattt tggcctggca
    17461 agtattgtaa atgcgaattc ttatcattca taatccaata gccggaaacg gtaaacgaac
    17521 aaatgtgctg gaaccgtttg aaaggaaagg gtttatcatt attgaaacca aaggaccaaa
    17581 tgatgccacc caaattgttc atgaaaatat tgccggttac gatttgttta tagcggcagg
    17641 aggtgatggc actgtaaatg aaattgcaaa ggcattgatt ggtacggaaa aatcaatggg
    17701 tataattccg atgggatcag ggaatggttt ggttaatgaa gttgggcaga ttacggtaaa
    17761 agcattgctg gaaaataatt acagaaccac aattatcgat tctctttttc taaatgaaat
    17821 tccttttttt aatgtggcag gaattggctt tgaggcgatt gttgcccatg aattcgctaa
    17881 acagaaaaag cgaggactga tcgggtattt caccaccacc atttccggaa ttttttccta
    17941 caagccgtta ataatcagtt attcttttaa tgacattctc aaatccgata aaattttttc
    18001 catcactatc gcgaactcaa agcaatatgg taataacgcg gtcatctccc cttcatcaaa
    18061 accaaatgac ggaattttcg aagtttgtat gattagaagc tttcccctgt tatatggacc
    18121 gattctcagt tatcgtctct tctcgcgtac tatttcaaaa ttcaaatact atgaggtaat
    18181 catgactaca tcttttcatt tgatgaacga aatttcaacg ctttggaatc ttgacgggga
    18241 atcaatacgc ctgcccggac ccttccatat ttctattaag ccggcttcat tgaaaatcat
    18301 ttccgaaatc ccaaactagt tctcaaaaca gtgaagcagt gagcagtgaa gcagtgaact
    18361 gtgagccact tgtgtaaaaa attataaatc catgggagtt ggtaagtctc tttagcagct
    18421 tttttaacta attttaaggt taatgagaaa actgctggca attgttttcg tttcattttt
    18481 cggtttccaa acagctggat tcctgatcgt attcgaactg cagaaatatt gcattcggaa
    18541 agaaataaag aatatgatta agcaaggatt gccagaggag gatcttgtag tttttagaat
    18601 tggggaagaa gaacagaaag cattccttaa gaattttctt ttttttgata aaaatgaatt
    18661 tcgattcgag ggtaagatgt atgatgtagt aagggttgaa atggaggcaa attttatcta
    18721 ctactattgt gtctctgatg aaaaagaatc tttattgttc gctaatcttg attcttcagt
    18781 tagaaaatct ctggatgatt ctccatctaa tcaaaaccag ctacgaaatt tcaatacctt
    18841 tatttcttca ctgtactatc cgcaaggaaa caatatcatc tttaagaatt tttctatagg
    18901 caaaaatatg tcgcattatt tctttagcgt tcaaagctgg aaaaactcac cttcaacccg
    18961 cccacccaaa tcatagtaat ttgttgcaag cccggttgct aaagcaactg agtaatctta
    19021 ctacattctc actattacac aacaatgaat aatatattcg tattttgttt gtgctttctt
    19081 gtacaacaaa gtttcgcgca aacattaacc atacgggacg ctgatactcg tcatccaatc
    19141 gaaatggtca cgctttatag ctcaaagcct gttgtgtcag catttacgga tgcgaaggga
    19201 aacgttgatc tgagtcagtt cgttgatgcc gacactatat acttcaggct cattggctac
    19261 aaacctgttc aaaaaactct gcccgaaatt cagcggatga attacatcct gttaatgaat
    19321 ccgacaaatt tcagtctaca ggaaatcaca atttcggttt caagatggga gcaggaaaaa
    19381 catgaccttc ccaataagat ttccagcata aaaccggaag agatttacct tcagaatcct
    19441 cagactgctg ctgacatcct tactattagc aacgaagttt atattcaaaa gagtcagttg
    19501 ggtggcggca gtccaatgat tcgtggtttc gctactaacc gcgttttgct ggtggttgat
    19561 ggagtcagaa tgaacaatgc catctttcgt agtggcaatc tgcaaaatgt aatttcattg
    19621 gaccctttta ctattcaaag ttctgagatt gtatttggac cgggctcggt tatctatggt
    19681 agtgatgcca ttggtggagt aatgagtttc tatacccttt caccttcctt ttcttccacg
    19741 gagaaaccat tggtaaaggg cagtgtattt acccgatact cttctgcgaa ctcagaatta
    19801 acaggacacc tggatcttaa tgttggcttt aagaaatggg gctcatcaac cagcatttcg
    19861 tattctaatt ttgaaaatct taaaatgggt aaacatggtc ccgatgaata tctccgccca
    19921 acttacgcgg tcagaattaa cggagttgac accatgctga tcaatagtga tcctgaaatc
    19981 cagattccta cgggatataa tcaggtgaac ctcatgcaaa agcttcgatt tgtcccaacc
    20041 cataattgga aaattgatta tggtttccat tattctacca cttcagattt tccaagatat
    20101 gatcgacaca tcctgtatag gaacggtttg caacgatctg ccgagtggta ttatggatct
    20161 cagacttgga tgatgaactc tttggggatt ttgaattcga gcgaaacatc tttttataat
    20221 caggtaaaac ttacactgtc ttatcagcac tttgaagaaa gtcggcatga tcgtgatttt
    20281 ggagatgtga ttcgatataa ccggtacgaa gaagtagatg cattcggtgc taatcttgat
    20341 tttgaaaaaa aaataagcaa gaagcattct attttctacg gtgtggaagg aatccttaat
    20401 gaagtaggct ctacaggaat tgataccaat atagcaactc agtcagagtc tcctggtcca
    20461 actcgctatc cggatggctc aacctggcaa tcttatgccg gatatattaa ttaccttgtg
    20521 cgcacttcga agttactgac tatccaaacc ggactccgtt ataatcatat tttattgaaa
    20581 tcgcagtttg ataccgcatt ttatccgttt ccattttcag aagccaacat taatgccggc
    20641 gccgtaaccg ggagtgcagg tttggcctgg tctcccctta gttcctggca attcaacgct
    20701 aatttttcaa gcggcttcag atccccgaat gtggacgatg tgggaaaagt ttttgactca
    20761 gagccaggat ccgtaatttt acccaaccct gatctgaagc ccgaatacgc ttataacttc
    20821 gagctgggaa taacccacat ttttggcgat ttggcgaagt tggatgcaac aggatactac
    20881 accatcctga aagatgccat ggttcgacga aattaccggc taaacgggca ggatagtatt
    20941 ctctatgacg gagagttaag ccaggtacta tcaattcaaa actccgcaaa ggcatttgtt
    21001 tatggcattc agacaggggt tgaagttaaa cttccagccg ggttcagtct tacttcaaat
    21061 attaattacc aggctggaga agaagaattg gatgacgaaa gcacggcacc attaagacat
    21121 gcggcacccc tgtttggctc tactcatata ctttggacca gaggtaaatt gaaagcagat
    21181 ttatactcat tttataatgg caggattgcc tttaaaaatt tggctccgga ggaacagaac
    21241 aaatcgtatc tctatgcaat tgatggaaat ggcaatcctt attcacccgc gtggtataca
    21301 ttgaatttta agtttttgta tcatgccacc aactttatca cattaaatct tggccttgaa
    21361 aatataacca accaaagata ccggccctat tcctccggta ttgcagctcc agggaccaat
    21421 ttcatcattt caaccagatt tactttttag ttatctttcc taaacattga aataaaaagc
    21481 aataatttgg caaccaataa ttgaattatt agtacacctt tagattgtct ttgatttgaa
    21541 gaaattatcc attcttattc ctgtttttaa tgaagaacgg accattttta acgtccttca
    21601 gaaagtaaca tccgtctctt taattaatga tattagtaaa gagattgtga ttgtggatga
    21661 ttattcaact gacaataccc gaagtgaaat caaaaaattt attcatgatt ttcctgaaat
    21721 ggaaattatt tttcttgaac accagaaaaa tagaggaaaa ggcgcagcga ttcgaacagg
    21781 aattgaacgt gcaacgggag attatataat tattcaggat gctgatcttg aatacgatcc
    21841 gaatgaaata aacaggttgc tacaacccat tgtggaagag aatgcagatg ttgttttggg
    21901 ttcaagattc ctttcaggca gttccaaaag agttttatac ttctggcact cactcggaaa
    21961 taagtttctg actttgttat ccaattttat gaccgacctg aatttaagtg acatggaagt
    22021 tgggtataag ttgattccca cttcaatctt taggtatatc aaaataaggg aaaatcgatt
    22081 tggattcgaa cctgaagtag ttggtaaact ggcagcgata aaaggaatac gaatttatga
    22141 agtcggtatt tcttaccacg gaagaactta tgaggaggga aagaaaatta actggaaaga
    22201 cggctttagg gccatttact gtattttccg atatgcaccg gtttttaaca gaagattgaa
    22261 aaattataaa aaagacccgc aaagtgcttt tcaaacggga tcaaaaacat aaacatctcc
    22321 caaacaaatc caggttgatt aagaaacttc atctctccca attcttacat agccttccgc
    22381 tattttgaaa gtagaaccgg ctgcaagtaa ttcaataata gaaagcctca agatttaact
    22441 aatacctata tgaaagcaag cctaaccatt ctgctactaa ttatttcgaa ttcattcatg
    22501 acattggcct ggtatggaca tcttaagttc tatgaaatga aaggatggga aaagctgagt
    22561 cttttttcag taatactaat tagctgggga attgcctttt ttgagtattg cttccaggta
    22621 ccggcaaacc ggattggatt ccagtctcag ggcggaccct tcacgctcgt ccagcttaaa
    22681 gttattcagg aagtaatcac tttagtcgtt ttttcacttt tcacaattat gttcttcaaa
    22741 acggaaacaa ttcgcttaaa tcactttatc ggatttattt tcctgattct tgctgtttat
    22801 ttcattttta aaaaataaaa aaaccatttc acaataaaaa tattggaagc cggacatatt
    22861 gtatccggtg agaattatac tcctttgagc acacaatcat tcagcaaaca actgagatgt
    22921 acacccatgt tgccgtaaat acttttaaaa aggttaaaaa tcctttagat tagttgacgc
    22981 tataaaaggg atatattcaa tagatatatc cggttgttgt aaccaattaa aaaaaatatc
    23041 aagtgaaaat aaacatctca attcttttgt tttttctttc aactgtctgt tttggacaaa
    23101 tcctaccgca aattgattca ttgattgaaa atgaaatgaa atcagacaaa gtcgctgcat
    23161 tggcagttgc agtaatcgat tctggcaaag tagttcacct taacacttat ggctttaggg
    23221 atttggaaaa taaatttaaa gcaactgtta atactccatt ccatattgcg tccgtttcca
    23281 agacggtaac aaatttggcc gtattcaatt tagtagaatc tggcaaaatt gaattggaaa
    23341 ccgatataaa tgcatatctc ccatttaagg ttaaaaatcc tcattttcca aatacaaaag
    23401 taaccgttcg tgatttgcta aatcaccgaa gtggaataag agatgatttt gagatttatg
    23461 aacccttttg gacaattcct aatggagatc caaccctaga attaaatgac tttttaaaga
    23521 attatttaag cgcaggagga aaactctata aagatggaca ttttgaaagc aattcaaatt
    23581 acaagtcttt ttcctattcg aatacgggag tagctttatt aggacttata gttgagaccg
    23641 tttcaggaat gaaatatgag gattattgtc aagaaaacat ctttaaacct attggaatga
    23701 tgaatacgag ttggttttta aaaaacttag actcaaatct ggttgcaaaa acttatgtgc
    23761 atgaagattc tatcggacta atttttaagg gacataatgg ctaccctgat tatcctggag
    23821 gtcaattaag aacatcaatt tctgattatt cattattgct aactggatat ttgaatgcgg
    23881 aaagctctaa cttcatatta gatgcaaata ccacgagaaa aataacccca ttaccacaaa
    23941 tttcccatga agggttttat acttggttcc ttaatgccat aaacgataat atttattatg
    24001 ctcatcctgg tggggatact ggtgtgagaa caattgtaat aatagatgtt agaaataaaa
    24061 atgggattgc ccttttttca aatgcagaga ttgaattgac caacttactt agagaaatag
    24121 aaaaaaaaat gtggagtaaa taacgggtta caacactgta taaaatgcat tttacaacta
    24181 aaacgttagc agtaataatt gcatgaagga cattgtaatt gagacagaag acaggtttgg
    24241 cctaaaagcg acaatttata aggcaagtaa aaattccatt aaaaactgta ccctaatcat
    24301 taattcagcg actgggatag gtagaaagtt ttattcgaga tattccaagt acatggcttc
    24361 aaacgggttt gacgtaataa cctatgacta ccgaggaata gcagagtcca gaccccaaaa
    24421 acttaaaggc tttaaagcgg actttgtgat ttggggggaa agggactttt catctgtcat
    24481 taagaaatca aagaatctac ttcctattaa caaaattgtt gttttagggc acagtatcgg
    24541 aggaacaatc tttgggatga ctctggactg ccaagaaatt gaggctgtaa taatgatagg
    24601 ggcgcagaca tcttattata aagattggcc aaagaagcat cgtgtaaagc tatatcttct
    24661 gtggcatatg gtaatgccat tcttaactaa cttggttgga tattttcctg gaaaaaaatt
    24721 aagaatgatg gaggatattc caaaaggagt tgtgagacaa tggaatgaaa gaaggaagaa
    24781 tcaatcaatg gcggctcaac ttcaggaaaa taatgtaaac cttttttatc acatctatac
    24841 caagaagttg ttgacaattt ttattgaaga tgatgttatt gggacgaaaa gagcggtaga
    24901 aagaattacg gaattattta tttgcgctga cagacaagtt ataagtgtaa gcccatcaga
    24961 ctactctgca agtgtcattg gacattttgg tttttttaaa aaggactttg agaaaaattt
    25021 atggttacag actgtaaatt ggtacacaga agcaattact actgccagca gtaagtttct
    25081 gccagcttga ggtgcaccgt aaatttatag tttgatttta ctaatttagt ttacgtttcg
    25141 tgggacaaca cgcggcttcg agaggccggc cacatcgcaa acacaaacgt tggcggtaat
    25201 tgccgaccac accggaaaac gactcaaaaa gatttggaga ttgttgtctg aataggtaac
    25261 tttgcccatg tgactaaatt tctccgtgag acatatgttt accaaaatca tttttgggac
    25321 ttctacaata accagaaaaa ggaggttcag gacaagattg attatgtaat cggactcgtg
    25381 aggactttac aaatggttcc agaaaggttt ttcaaacatc ttgagggaac tgatggcatt
    25441 tacgaaatga gagtaaaagt gggttctgat atttatagaa tcttctgttt cttcgaccaa
    25501 ggacaactaa tcattttact aaatgggttt caaaaaaaga aagataaaac tccgagacaa
    25561 gaaattataa gagcagaacg gcttaaaaag gaatattatg aaaacaaaat taaaagataa
    25621 gtacctgact tcatgggacg accatcttga caagaagtat ggaaaaaaag gatcaaagag
    25681 tcgtcagaaa tatgaagaag attttgagac atttaaaatt ggaattctca tacaagaggc
    25741 gcggaaaaga cagcatatga ctcagcagga actagcaaat aaagttggga caacaaaaaa
    25801 ctatatttca aggattgaga ataacgctag tgacattaga ctgacaacgt tgatgagaat
    25861 tattcgagag ggacttggtg gccgactaaa attctcggtt gacatataga gagtgaaggc
    25921 cactaccgcc aatcgaatag cccgccccgc cagttgtaac tgaggagatg tgcctctcac
    25981 acctatcccg acgcgtcggg acggttcgtt aatactgcaa ccgctcttta taccagctac
    26041 agcttgttct actttgaaca agggctggct aacccgaggt ttgaacctcc ctatctccac
    26101 acgaaaaaaa atttggtggt ttgccaaata gttcatagtt ttgcgaacta ttaaactttt
    26161 aaacaatggt cgacaagaag atttttgaac tacacgcaga cgtttgtaaa gcattgggac
    26221 accctttgcg tattgaggtt attgacctgc tgcaagacaa agagctttgc tttactgata
    26281 ttctggaagt aacaggtgga ttaaaatcaa acctctccca aaatctttct gtaatgacaa
    26341 aaaagggtat tttaaaatcg agacgggatg gacaatgcac ttacttttcg ctttcttcca
    26401 aaaaagtagc tcaggcgtgt cgactaatgc gtggggttct tattgacagc cttaaaaaac
    26461 aaaaaaacct ccttgaaaaa atgtaatctt cagaaaaatg ggcaaaacaa tattggtctt
    26521 aggagcagga actggtggaa ttatcactgc taaagaatta agtaaaaaag tcggtggtaa
    26581 aaccaaaata ttgctttttg aaaaagaaga aaaaaatatt tttgctcctt cccttctttg
    26641 gttgatggtt ggtaagcgga aaccgaaaca ggtttacaga aacacccgaa aaattacagg
    26701 tgcaggcatt gaagtaatta tcggagaaat tgaaaaagta aatcccgaga aaatttccgt
    26761 taccgtaaaa ggtcaggaat atgaagggga ttatatgatt atttcgcttg gagttgagca
    26821 aatagcagaa cataatctga atgactacgg acacgatttt tacacccttg acggtgcagc
    26881 aaaattcaat gaacaattgc aaaatttcaa aggtggaaaa attgcggttt tagttccgtc
    26941 cattcctttc aaatgtcctg ctgcacctta tgaagctgca atgcttgtag aaaattttat
    27001 cagaaaaaag gagttgaaca gtaaagcaga agtttcgtta tacgttcctg aacctggtcc
    27061 tatgggtgtt gctggaaaag aattatcggg agcggtcagg caaatggttg aaatgaaagg
    27121 aataaaatat tttccagaac atcaactaac atcagccaca gaaagaattt tgacttttaa
    27181 taatggtaaa actttcgaat atgatttaat ggcgttcacc ccaaaacatc aatgtccttc
    27241 ggtaatcagg gaaaccgctt tagttggaaa gtcgggttgg attgaagtga accgtaatac
    27301 aatggaaaca aattttccga atgtttacgc tatcggggac attacattta ttccacttga
    27361 aattggcaaa ccactaccaa aagcaggagt gtttgcacac tatcaggcag aaaccgttgc
    27421 acacaacatt gctcaaaaaa ttgcaggaaa atcttccggc aaaactttta atggtgacgg
    27481 gcaatgcttt ttagaaatgg gcggcggaaa tgcgggttat gcaggtggta acttttacgc
    27541 ttcgcctgtg ccggatgtaa aaatgaaaaa gcccggctat ttctggcact ggacaaaagt
    27601 ttggtttgaa aaatattggt tcttcaaata cttttaaaat tataagtgta gtaattacag
    27661 caaataaagt ttatccctgt tcgacatgca ggcataaaaa tgagaaacaa aatgaaaatt
    27721 ttaatcttaa tcaatgatgc tccctacggt acagagaaag catacaatgc tttacgactt
    27781 gcaaatcaat taaacaaaga ccacgaaacg gttgaagtga gagttttttt aatggcagat
    27841 gctgccagtt gtgccgttgc taatcagaca actccgaatg gttattataa cattgaaaga
    27901 atgctgaaac tttcgctaaa caaaggaaca aaagtgaaaa tatgcggaag ttgcgcagac
    27961 gcaagaggtc taaaaaatgt tcaactcatt gaaggaacag aaatcagcac aatggctgaa
    28021 ctgaccaact gggttgtgga cagcgataaa gttctcatat tctaaaaccc cgcttctttt
    28081 tagtcataca ttttgtgctc caaaaccctt acgtttctta gccaaaaccg gttgttgggc
    28141 aattgtgaaa cacttaaaaa tactgttgac aatgacaaaa cggttcagac aactgagaac
    28201 actttgaaat atctttcact aaaccatatg gcatggctac taccggccac tattttggtt
    28261 caccggctgg aagaatactt tggccaattt ccacgttggt attccaatct ttttaaatac
    28321 acagtcgtcc aaccaggatt tcactgttat caatggagtt ggcctgttcg tattcaccgt
    28381 attttccttt tcctatattt tcgatagtta ttgcagacaa ataaaaaagg tatttacgtt
    28441 gttgacaaac actttggaca gaggatgata tttgaaccaa tataatattc aatcaatttg
    28501 ttaaattaaa ttcttgagtc agcttgttct acttagaaca tagggactgt aacctggaaa
    28561 ttttggcttt gcgaaccgac tttttgacta aatttaaaca ctgacttttt atgatatggc
    28621 gacagaaata aaatataaaa ttaaggccga aaacgtttcg acagttcaaa atcttttcaa
    28681 tccctgcaac gttggggcaa gaccgttttt aaagtgggct ggaggcaagg gtcaattact
    28741 cgacaaattt caagaactct acccaaagca actcaagcag aaaaaaatta aaaattttta
    28801 cgagccattt ttgggcagcg gtgccgtatt ctttgacatt gcacagcact atgaaattga
    28861 aggcgcatat ctctacgaca ttaacgatga acttattttg acttataaag ccattcaaaa
    28921 agacgtaaca aaactgcttg actttcttta tcgctaccaa aaaaaataca tcaaacttga
    28981 caaaaaacaa cgccaggaat ttttctacga ccaacggaca aattacaact tgcagcgttt
    29041 caatattgat tacgacaaat a
//
LOCUS       DASWHX010000136         4800 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_1232316, whole genome shotgun sequence.
ACCESSION   DASWHX010000136 DASWHX010000000
VERSION     DASWHX010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 4800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4800
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1232316"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            218..664
                     /locus_tag="VGA21_09350"
     CDS             218..664
                     /locus_tag="VGA21_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015345565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650756.1"
                     /translation="MKYDKYQLESDRKLLLFEFTSVGPKGKIKKIVQYTETNLKDYYN
                     LGFGDKNEKAGEIDDTAITNNGDSQMVLSTVASTVYAFTDKHPDAWIYAIGSNSARTR
                     LYRIGITNNILEIKKDFEVFGLKDGQWHEFRKHTDYKAFLIRRKKS"
     gene            complement(1261..2472)
                     /locus_tag="VGA21_09355"
     CDS             complement(1261..2472)
                     /locus_tag="VGA21_09355"
                     /inference="COORDINATES: protein motif:HMM:TIGR01976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase-like protein"
                     /protein_id="HEX9650757.1"
                     /translation="MQIQKSTFLQARQHFPALARNMKGNPVIFLDGPAGTQVPAQVIE
                     AISHYYKTSNSNTHGFFRNTRETDQIIHEAREWSAKFFGAEGPETISFGQNMTTLAYF
                     LSAGFGRILKPGDEIIITQLDHEANRGPWLVLKESGITIVEVNITAQGILDMDDFQKK
                     LSERTKLVAVGCASNALGTVNDVKTIREMSKRVGAKLLLDAVHFAPHFPINVMELDCE
                     FLLASAYKFYGPHVGILYSKPGELDILPVDSLRTQDQIPPSKIETGTLNHAAIAGVTA
                     CIKFIMEMAGEDTPQKAMKLLAVHERILLEKLFHGLSEVPSVKIQGPGLDYPRTPTVS
                     FTHDKYRPEVVCEKLGEMGIFAWDGHFFAIRPIEALGLLERGGVTRLGISMYTTEDEI
                     EKTIQAMLQLH"
     gene            2602..4212
                     /locus_tag="VGA21_09360"
     CDS             2602..4212
                     /locus_tag="VGA21_09360"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX9650758.1"
                     /translation="MLNKLYKRFDPDADFNELDYAIAGSGVGGLTAAIFLAKAGKKVA
                     VFERHWVPGGFTHTFKRKNGFVWDVGVHYVGNMDSEDSQLRVLFDYLTDNKLKWESMG
                     NVYDKVIIDGEEFSFDAGKDNLTAALYRQFPAEKDAIDSYFKLLKKAEKTSKLFFYQK
                     VFPDWLRNTLGKIFLYFCKPYYSRTTYEVISELTGNEKLISVLCAQCGNYGLPPKSSS
                     FAAHAMVINHFMEGGYYPVGGADKIYKGLIENLNMYNGQVFINSDVEEIVVENNKARG
                     VIVNGKFIKSKNVISNCGIRNTLTKLIKHGIKEEWLKQLDQVNPSHPHMCLYLGLDSS
                     DKDLKLPKNNIWFYRNHNFDEITNETLSDKDGELKFAYISFPSAKDPSWEKDHPNMAT
                     IQAISIANFDWFKDYEDRQWRHRGPEYEAIKENFKDKMLKKLYELLPQIKGHIVSSDV
                     STPLTTKDFTNYASGEIYGLEHSPKRFKLSFLRPRIGIKGLYLVGQDISIVGVAGAML
                     SGLMCAISILKFRVFRQFREMAKNKNAA"
     gene            complement(4410..>4800)
                     /locus_tag="VGA21_09365"
     CDS             complement(4410..>4800)
                     /locus_tag="VGA21_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020529400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650759.1"
                     /translation="IWDKMTGEINPEVAEFWKENYDLRYILERDWEKLGADLQGKIHI
                     FCGDMDNYYLNNAVYLMEEFLESTTNPYYEGEVDYGDRAEHCWNGDQENPNAISRLRY
                     NTMYLPKILERINKSAPKGSDTKSWRY"
BASE COUNT         1468 a          936 c          966 g         1430 t
ORIGIN      
        1 tttccgttga aaagactctt atattcgtca ttcaaggcgc acacaatgta tataaaaaat
       61 aggcgaaata gtgctgtatt caaggttttt ggctcgtatg aaagtttgct cttaaccgaa
      121 agtttagtgc ttcgaaatcg cctacttttc atatactaat cgttatcggt aatgcgagca
      181 gaccgtaaaa cgagaaaatt actaccttta gactaacatg aaatatgata aataccaact
      241 cgaatcagac aggaaattat tattatttga atttacaagc gttgggccaa aaggtaaaat
      301 aaaaaaaatt gttcaataca ctgaaaccaa tctaaaagac tattataatt tggggttcgg
      361 agataaaaac gaaaaggctg gtgaaattga tgacaccgca atcacaaata atggtgacag
      421 ccaaatggtt ttatcgaccg ttgcttcgac tgtttatgca ttcacagata aacatcctga
      481 cgcttggatt tacgccattg gtagtaacag tgcgcggaca agactttatc gaatcggaat
      541 taccaataac atactagaaa tcaagaaaga ctttgaagta tttggcttaa aagatggcca
      601 atggcacgaa tttcgtaagc atacagacta taaagcattt ttaatccgaa gaaaaaagtc
      661 gtaaatttgc actatgacaa tgactataaa gcagcttaat aaatccaaaa ctcctattgt
      721 taaaatcaat aaggccttag ataaatttgc tgacaaagtt ttattcccag acaaacttga
      781 agaagcaaat cgcattctca agacggttgg tttacctaag accaaaaaat cgcacaaccg
      841 ctaacatata aggcttaaac gaagtccagc cacaaagtat accgtgttac gaagctgacg
      901 gctgtcatgg ctggggttcg atgtaagcct gtgttgaaac tacctgtccg gtttacctgc
      961 cggaggcatg gcaggcaggc tgaagctttg gtagtttctg gcgtttttgt tatgggattt
     1021 caaaacagaa gattggctgt ttttcataga aaaaagtgag taaaaatttt catccaggga
     1081 ataattgcca tcgggtaggt aaagtagaaa gcggaagttt ttcaatttta cttttcaaag
     1141 tcattattca attatttttt tgggtacagg actataatta actaatgtaa taatagcaat
     1201 attcgtttca taccaaaata ttaattggga gaatgaagat aataaaaatt ggtttaccct
     1261 tcaatgtaat tgaagcattg cctggattgt cttttcaatt tcgtcttctg ttgtgtacat
     1321 ggaaataccg agtcttgtaa cgccgccacg ctccagtaat cctaaagctt caattggcct
     1381 gattgcgaaa aaatgaccat cccatgcaaa aatccccatt tcgcctaatt tttcacaaac
     1441 tacttcaggt ctgtatttgt catgtgtgaa agaaactgtg ggtgttctgg gataatccag
     1501 acccggcccc tgaattttga ccgaaggaac ttcagacaat ccatggaata atttctccag
     1561 gagtattctt tcatgtacgg cgagtagttt catggctttt tggggagtgt cttctccagc
     1621 catttccata atgaatttta tgcaggccgt tactccggca attgctgcat ggttcagtgt
     1681 gccggtttca attttactgg gcggaatttg atcctgagtc cgaagagaat caaccggaag
     1741 gatatccagt tcaccaggct ttgagtaaag aataccaaca tgcggtccat aaaacttata
     1801 tgcagaggcc aacaaaaact cgcaatctaa ttccataaca ttaattggaa aatggggcgc
     1861 aaaatgtact gcatccaata gtagttttgc tcccaccctc ttagacatct cacggatggt
     1921 ttttacatcg ttcaccgtac ctagcgcgtt tgaagcacaa ccaaccgcca ccagttttgt
     1981 cctctcactc aatttctttt gaaaatcatc catgtcgagg attccttggg cggtgatgtt
     2041 cacttcaaca attgtgattc cgctttcttt aagtaccagc cacggacccc ggttggcttc
     2101 atgatccagc tgggtaataa ttatttcatc gcccggttta aggattctgc caaagccggc
     2161 gctgagaaaa tatgcaaggg ttgtcatatt ctggccgaag gaaatagttt ccgggccttc
     2221 agcaccgaag aatttcgcgg accattcacg cgcctcatga attatttgat cagtttccct
     2281 ggtattcctg aaaaatccat gggtgttgga attactggtt ttatagtaat ggctgatggc
     2341 ttcgatcacc tgagcaggta cctgagtgcc cgcgggccca tccaggaaaa tcaccggatt
     2401 gcccttcata tttctggcta aagcgggaaa atgttgtcga gcttgaagaa atgtggattt
     2461 ttggatttgc ataatcgcta ttttgtaatc tatttacttt tccagtcttg attttgcaat
     2521 agtaaaaagg atatttatta attctcaaac cctaatagta atatcattgt tataaattgc
     2581 gttctcccct tcagtacagc aatgctaaat aaactttata aaagatttga tcctgatgcc
     2641 gattttaatg aattggatta cgcgattgca ggctccgggg tgggtggact tacagcggca
     2701 atttttctgg ctaaggcggg gaaaaaagtg gcggttttcg aaaggcattg ggtccctgga
     2761 ggtttcactc atacttttaa aagaaaaaac ggatttgtct gggatgttgg agtccattac
     2821 gtcggcaaca tggatagtga agattcgcaa ctcagggtat tatttgatta tctcaccgat
     2881 aacaagctta agtgggaatc catgggcaat gtttatgaca aagtgattat tgatggtgaa
     2941 gaattttctt ttgacgccgg aaaagataat ttaactgcgg cattatacag acaattccca
     3001 gctgaaaagg atgcaattga ttcctatttc aaattattaa agaaggccga aaagaccagc
     3061 aaactcttct tctaccaaaa agtatttcct gattggttga gaaataccct ggggaaaatt
     3121 ttcctttact tttgcaaacc ctattattcc agaactactt atgaggttat cagtgaactc
     3181 accggtaatg aaaaattgat ttcagtgcta tgtgcccaat gcggtaacta cggcctccca
     3241 ccgaaaagca gcagctttgc agcacatgcc atggttatta atcatttcat ggagggtggt
     3301 tactaccccg tgggaggtgc cgataaaata tataaaggat tgattgaaaa tctgaatatg
     3361 tacaatggac aggtttttat taattccgat gttgaggaaa ttgtggttga aaacaataag
     3421 gcccgcgggg tgattgttaa tggcaagttt attaaatcca aaaatgttat cagtaattgc
     3481 ggtatccgca atactttaac aaaattaata aaacatggca tcaaagaaga gtggttgaag
     3541 caattagacc aggtaaatcc ttctcatcct cacatgtgtt tgtatttggg tcttgattcc
     3601 agcgacaaag atctcaagtt gccaaaaaat aatatctggt tttaccggaa tcataacttt
     3661 gatgaaatta caaatgaaac tttgtcagat aaagacgggg aattaaagtt tgcttacatt
     3721 tcatttccat cagcaaaaga tccttcctgg gaaaaggacc atccaaacat ggcaactata
     3781 caggcaatca gcattgccaa ctttgattgg ttcaaagatt atgaagacag acaatggcgg
     3841 caccgggggc cggaatatga agccatcaaa gaaaatttca aagacaaaat gctgaagaaa
     3901 ttatatgaat tactacctca aatcaaagga catatagtga gttcggacgt gtcaacaccg
     3961 cttactacaa aagactttac gaattatgct tccggagaaa tttatggact tgaacatagc
     4021 ccaaaacgat tcaaattaag ttttctccgc cctaggattg gtattaaagg tctttacctc
     4081 gtgggtcagg atatttccat agttggagta gccggcgcca tgctttcagg ccttatgtgc
     4141 gcaatctcta ttttgaaatt cagagttttc aggcaattcc gggagatggc gaaaaacaaa
     4201 aatgcggcct gacatttcaa cttagttttt tgtaacttca tattgtgaaa cgcatttttt
     4261 tacaaaaaaa aatatggcct ttgctgctta ctcttgtgat tgcagttctt gcgtcatgtg
     4321 tgagtaataa gccgcatgga aaactaaaac cgggaaagcc aattccctgt ccatgtgacc
     4381 actcttagtt tggatttaat agttccacat cagtatcgcc aactctttgt atccgaccct
     4441 tttggtgccg acttattaat tctttccagg atttttggta gatacatggt attatatcgc
     4501 aaacgactga ttgcatttgg attttcctgg tctccgttcc agcaatgctc ggctctgtca
     4561 ccgtagtcaa cttcaccctc ataataaggg ttggtagtag attccaaaaa ttcttccatc
     4621 aggtaaacag cgttgttcag gtagtagtta tccatatctc cacagaaaat atggatcttg
     4681 ccttgaagat ccgcacctag tttttcccaa tcccgttcca ggatgtaacg caaatcgtaa
     4741 ttctccttcc aaaactccgc aacttccgga ttgatttctc cggtcatttt atcccaaatc
//
LOCUS       DASWHX010000137         8496 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_2367356, whole genome shotgun sequence.
ACCESSION   DASWHX010000137 DASWHX010000000
VERSION     DASWHX010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 8496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8496
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2367356"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            29..685
                     /gene="truB"
                     /locus_tag="VGA21_09370"
     CDS             29..685
                     /gene="truB"
                     /locus_tag="VGA21_09370"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012472569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEX9650760.1"
                     /translation="MSVEEQDKIILIDKPIGWTSFDVVKKIRNCLGIRKVGHAGTLDP
                     LATGLLILCTGKKTKEINEIQEMEKEYVGEFTLGQITDSFDLETPVKFVSDCKNVTPD
                     SVVETAKKFVGTVNQVPPAFSAIKIEGKRAYLKARKGEKQSIQPRKVTLYSFEILKIE
                     IPLIAFKVICSKGTYIRSLANDFGNEIGAGAYLSRLKRTRIGTYNLEDAATIEQICLQ
                     "
     gene            682..1614
                     /locus_tag="VGA21_09375"
     CDS             682..1614
                     /locus_tag="VGA21_09375"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009583728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEX9650761.1"
                     /translation="MKVYNSIEEFTPLNFAVVTSGTFDGVHYGHQQILRKVSKIATEN
                     NGESVIITFWPHPRLVLFPDQQDLKLLTTFEEKVQLLTDLGIDHLLKIPFTREFSELS
                     SQEFIDQILIKILGTKKLVIGYDHRFGKNREGSFDHLVANSSRYGFQVVEIPKQEIDS
                     VAVSSSKIRKALQEGKIPEANEYLGRPYSLEGIVIKGNMLGRKIGFPTANIEVRDEYK
                     LIPADGIYAARITSRQKQFGGMLYIGNRPVLQQSQRSIEVNIFDFNEDIYGEIITIKM
                     IRRIRDEGYFKSMEELGLQLAKDKEIVKQILLKE"
     gene            1620..3593
                     /locus_tag="VGA21_09380"
     CDS             1620..3593
                     /locus_tag="VGA21_09380"
                     /EC_number="6.2.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012928907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030729 - acetoacetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate--CoA ligase"
                     /protein_id="HEX9650762.1"
                     /translation="MDQPKLLWQPSTEFRDKSNLSGYRKWLAESKNISFVDYHALWIW
                     STEYIEAFWKSIWEYFDLEGSLEAIEICSRDSMPGVKWFEGTQLNYAREVFRNRNNEY
                     PALIFASEKSSIVEISWTELIEKTASLQAHLEKAGLKSGDRVAAFLPNIPEAIISFLA
                     VNSIGAIWSSCSPDFGLGAVTDRFTQIEPKILITVDGYQYAGKSYDKKDVINDLISKL
                     PSLEQIIYIPYLDDSAYNFAVNTSQWESVISESKQSLKFSETGFSHPIWVLFSSGTTG
                     VPKAITHSNGGIILEHLKYLSFHNNIKAGDRCFWYTTTGWMMWNYIIGSLLVKGTTVL
                     YDGSPAYPDMNVLWKFAEQAKITHFGTSAGFVIANMKAATRPGNNFNLNQLISIGSTG
                     SPLPPEGFDWLYKNVKSDLWVASISGGTDVCSAFVGGNPLLPVHSGEIQCRALGCKLE
                     AFDDEGRSIINQVGEMVLTKPMPSMPIYFWGDENFAKYTESYFEMYPGIWRHGDWTMI
                     TERNGIIIYGRSDSTLNRGGVRIGTSEIYRAVDKVDEISDSLIICIENNVGDFYMPLF
                     VTVRQGAKLDEILKNKIKTTIRSEYTPRHVPDEIIEITEVPYTISGKKVETPVKKILQ
                     GKPFEKILNKDALKNPAALDFFIEYAKKLKFHQ"
     gene            3590..3937
                     /gene="gldC"
                     /locus_tag="VGA21_09385"
     CDS             3590..3937
                     /gene="gldC"
                     /locus_tag="VGA21_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017730834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldC"
                     /protein_id="HEX9650763.1"
                     /translation="MIKSQIKFDVELDKDRIPEKIEWSATDHKDGKPEPINSFSVSLW
                     DSEQRNTLRIDLWTKKMPVDQMKQFCIDSIGGMAQTILNSTGDEYMANELKELCKKFS
                     IHLEKEIKEGKYR"
     gene            4006..6798
                     /locus_tag="VGA21_09390"
     CDS             4006..6798
                     /locus_tag="VGA21_09390"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX9650764.1"
                     /translation="MKQTLLKTHLGTFVALLISVTAWAQTTISGKINEASGDPLVGVN
                     IVVKGTIKGTITNINGNFNLSVSQSPPFTLSISYVGFITQEIEITETSTSGLGITMAE
                     QTLLGQEIVVSASRVEESILSSPVTIEKLDILAIQQASAPDFFDQLNYVKGVQTAQGS
                     MTFNAVNTRGFATIANTRFVALVDGMDISAPLLNFPTGNLVGIPDLDVESVELVPGAA
                     SALYGPNAFNGILFMNSKNPFEYQGLSAQAKLGVTESNANGTNPMYNFQLRYAKAFNN
                     KVAFKVNFSIMEATDWRANDYTTDKNFPIRAPGDSNFDGLNLYGDEVEIFYGGLGPFL
                     GALDLRRTGWKEEDLLETDAARSIKADAALHYRLTDKIEALYNYRFGGGRSIYQGSGK
                     FILRDFTQQFHKLELKSDNFFVRGYMTQTDDGDSYNMDALGIYLNESISPTATEWAPI
                     YIPAYVLALQGYYTALGVPAGNKQAAHAFARQTADATRPAVGSSQFTNLVETIRTTKF
                     NEGAGAAFVEGSRLYHGEFNYDFKNMIDIVDIQIGGNFRQYDIFSDGTIFDEEQNADG
                     SYERVKINEYGGYVQLGKSIGNLKLAASARYDKNENFEGQFNPRVSAVYTAGGSHNFR
                     ASFQTGFRNPDSQSQFIWFPTASGILIGSTEANAARYGLHNGGAYTNRSVLSFFDAGG
                     SFDTDGTPLDADGNPTDPVSAGLATDNFDYVQPEKLQAFEVGYKGIIGGDLLIDLNYY
                     HNIYNDFIAQKTVYNKLPIMRRGQVVTGATGDIITEFRPYLNAQEKVTSDGIGLGFTY
                     NLGRGFNFNGSYDYATFNVSKVDLNSEFEAGFNTPEHKFMVGFANRDVGENIGFSVAF
                     RWQDEFLWQSAFGEGTIPSYGTLDFQINKKLKALKSVIKVGATNLGGQDYRTNLGAGF
                     VGTIYYISLTFDQFLN"
     gene            6814..8355
                     /locus_tag="VGA21_09395"
     CDS             6814..8355
                     /locus_tag="VGA21_09395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650765.1"
                     /translation="MKTNIFRYLVLAILIGGCNQEFDEVIPDRTDLGSGHETFSAGPL
                     DFTTFVAIGNSLTAGYQAGALFTEGQSNSLPLILSKQFARVGGGTFNQPTINTVNGYS
                     GMSGSTVFGRLLLQLVGNPPTPVPTPQLSDGGAIPNILVNPSFAYSGNKATLNNFAVP
                     GIQVGQALIPETGDWNNSGHPAFNPYYARFASAPGTSTILGDALTRNPTFFFFWLGNN
                     DILGYAVGGASNEAIFTSTDNFTAYYGAALGALTANPAVEGIVGNIPNVTSIPFFKLV
                     PWNAIPLDTDKATALNTNPNFQDLNGAVTAGNNLGFISDAEAASRMLSWQAGQNGALI
                     IDEDLFDLRNLDPNIPELYAKVRLANSTDLLPLTAGTVLGVYLVPGNDATIQGVSYPV
                     GDQFALTASEQTAIADRTIAFNNIIKSILDGINAGGKRVALFDANARLAGLNTVPTYI
                     SNVMVDATLAPPFGAFSEDGVHPNSRGYALAAYWIIQQINQEFGSNVPRPQISEYQGT
                     GLPIN"
BASE COUNT         2635 a         1652 c         1847 g         2362 t
ORIGIN      
        1 cgcgttgatt tttcattacc ggttttagat gagtgttgaa gaacaagaca aaatcattct
       61 gatcgacaag cccattggct ggacttcctt cgacgtagta aagaaaatta gaaattgtct
      121 gggaatcagg aaggtaggac atgccggcac tcttgatcca ctggcaactg gattacttat
      181 tctttgcaca ggaaaaaaga caaaggaaat taatgagatt caggaaatgg agaaagagta
      241 cgtaggtgaa ttcactctgg gccaaataac cgattccttt gatcttgaaa ctccggttaa
      301 attcgtatcc gattgcaaaa atgttactcc tgattcagtt gttgagacag ccaaaaagtt
      361 tgttggcaca gtaaaccagg taccaccggc attttcagca ataaaaattg aaggcaaaag
      421 agcttacctg aaggcccgga aaggagaaaa gcaatcgatt cagcctagaa aagtaactct
      481 atacagtttt gaaatactta aaatagaaat tccgttaatt gcttttaaag taatttgctc
      541 gaaaggtact tatattcgaa gtttagctaa tgattttggt aatgaaattg gtgcgggtgc
      601 ttatctttcc aggttaaaaa gaacaaggat tggaacttat aatctggagg atgcggcaac
      661 tatcgaacaa atctgtcttc aatgaaagta tataatagca tcgaagagtt cacgcccctt
      721 aactttgccg ttgtcacaag cggaactttc gatggagttc attacggtca tcagcagatt
      781 cttcgaaagg tttcaaagat agccacagaa aataatgggg aatctgtaat aataactttc
      841 tggccacatc cccggcttgt cctttttccc gaccaacagg atttaaaact gctaactaca
      901 tttgaagaaa aagttcagct cttaactgat ttgggaattg accatttgct gaaaattcct
      961 tttacccggg agttctcaga attaagctca caggaattca ttgatcagat cctgataaaa
     1021 atactaggaa caaaaaaatt agttattgga tacgaccatc gatttggtaa aaacagggaa
     1081 ggcagttttg atcacctggt cgccaattct tccaggtatg gattccaggt ggtagaaatt
     1141 ccaaaacagg aaatagattc tgttgccgtc agttcatcta aaatcaggaa agcgttacag
     1201 gaaggcaaaa tacccgaagc caatgaatac ctgggaagac catatagcct cgaaggaatt
     1261 gttataaaag gaaatatgct aggtagaaaa attggatttc caacagccaa tattgaagtc
     1321 agagatgaat ataaattaat tcctgctgat ggcatttatg cagcccgtat aacttcaaga
     1381 caaaagcaat ttggaggaat gttgtatatt ggtaatagac ccgtgttgca gcaatcccaa
     1441 agaagtattg aggtaaatat ttttgatttt aatgaggata tctacgggga gatcatcacg
     1501 attaaaatga taagaaggat tagggatgag ggctacttta aaagtatgga agaactggga
     1561 ttacaactgg caaaagacaa agagatcgtt aaacagattt tactcaagga ataaacgtaa
     1621 tggatcagcc aaagcttctg tggcaaccct caacggaatt cagagataag tcaaatcttt
     1681 ccggatatag aaagtggtta gctgagtcaa agaatatttc atttgttgat tatcatgcat
     1741 tatggatttg gtcaacagaa tacattgaag cattctggaa atctatttgg gaatatttcg
     1801 atctggaagg aagtttggaa gcaattgaaa tctgttccag ggacagtatg cctggagtta
     1861 aatggtttga aggaacccaa ttaaattatg ccagggaggt tttcaggaac cgaaataatg
     1921 aatatcccgc tttaattttt gcttccgaga agtcttcgat tgttgagatt tcgtggactg
     1981 aattaattga aaaaactgct tcccttcagg cacatttgga aaaggcaggg ctaaaatccg
     2041 gtgacagggt ggcagccttt cttcccaaca ttccggaagc aataatttca ttccttgcag
     2101 ttaattcgat cggagctatt tggtcaagtt gttcgcctga ttttggtctc ggagctgtca
     2161 cagacagatt cacacagatt gaacctaaaa ttctaataac agttgacggt tatcagtacg
     2221 caggcaaaag ctatgacaaa aaagacgtaa ttaacgatct aatatcaaag ctcccttccc
     2281 tggagcaaat tatatacatt ccctatctgg atgatagtgc atataatttt gccgtaaaca
     2341 cctcacaatg ggaaagtgta atctctgaaa gcaaacaaag ccttaaattt tctgagaccg
     2401 gctttagcca ccctatttgg gtactgtttt cttccggaac taccggagtc ccaaaagcta
     2461 taactcacag taatggagga ataattcttg aacatttaaa atatctcagt tttcataata
     2521 atataaaagc aggagatcga tgtttttggt atacaactac aggttggatg atgtggaact
     2581 acattatagg ttcactactt gtgaaaggga ccacggtact ttatgacgga agccccgcct
     2641 atccggatat gaatgtgctt tggaaatttg cagaacaagc taagataaca cattttggaa
     2701 ccagcgccgg atttgtgatt gctaatatga aagcagcgac cagaccaggc aataacttta
     2761 accttaatca acttatatcc attggttcaa ccggttcccc tctgccgcca gaaggattcg
     2821 attggctcta caagaatgtt aaatccgatt tgtgggttgc ttctattagt ggtggcacgg
     2881 atgtatgttc agcatttgtt ggtggaaatc cattgttgcc cgttcatagc ggtgagatcc
     2941 agtgcagagc tttgggttgc aagctggaag cgtttgatga tgaaggccgt tcaataatta
     3001 atcaggtagg tgaaatggtg ctaacaaagc caatgccatc catgccaatt tatttttggg
     3061 gtgatgagaa ttttgcaaag tacacggaaa gttacttcga aatgtatcct ggtatttggc
     3121 gacacggaga ttggaccatg attacagaaa gaaatggcat cattatctac ggcaggtcgg
     3181 attcaacact aaatcgcggt ggggtaagaa ttggaaccag tgaaatatac agagctgtgg
     3241 ataaagtgga cgaaattagt gacagtttaa ttatttgtat tgaaaataat gttggcgact
     3301 tctacatgcc gttattcgtt accgttaggc agggagcgaa actggatgaa attcttaaaa
     3361 ataaaattaa aactaccatc aggtcggaat atactccccg tcatgtgccg gatgaaataa
     3421 tagaaattac cgaagttcct tataccataa gcggtaagaa agtcgaaacc ccagtcaaga
     3481 aaattttgca gggtaaacct ttcgagaaaa tacttaataa agatgcactt aaaaaccccg
     3541 ccgcccttga cttctttata gaatacgcta aaaagctaaa atttcaccaa tgattaaatc
     3601 tcagataaaa tttgacgttg aactagacaa ggatagaatt ccggagaaaa tagagtggtc
     3661 agccacagat cacaaagatg ggaaaccgga accaatcaat tcttttagtg tttcattgtg
     3721 ggattctgaa caaagaaata ctcttcgaat agatctatgg acgaaaaaaa tgcctgtaga
     3781 ccaaatgaaa cagttttgca tcgattctat tggcgggatg gcgcaaacaa ttctcaattc
     3841 tacaggagat gagtatatgg ctaatgaatt gaaggagcta tgtaaaaagt tttcaattca
     3901 cctcgaaaag gaaattaaag aagggaaata ccgctaatcg cgaaggaata ttgaaatatt
     3961 ccaataattt gctattttca ataattaatt accaatcaaa aaaagatgaa acaaacctta
     4021 ctaaaaaccc atctggggac gtttgtggca ctattaatca gtgtaacggc ctgggcacag
     4081 actacaattt caggtaaaat aaatgaagca tcgggtgatc ccctggtggg agttaatata
     4141 gttgtaaaag gaactattaa aggtaccatc acaaacataa atgggaattt caatttaagt
     4201 gtctcacagt caccgccttt caccctgtca atttcatacg tgggatttat tacccaggaa
     4261 attgagatta cagaaaccag cacctcaggc cttggaataa ccatggcgga gcaaacctta
     4321 cttggtcagg aaatcgtagt aagtgcaagc cgggttgaag aaagtattct ctcctcccct
     4381 gtgaccattg agaagctgga tattcttgca attcagcaag catcagctcc cgattttttt
     4441 gatcaactga attacgtgaa aggcgtgcaa actgcccagg gaagcatgac attcaatgcg
     4501 gtgaatacgc ggggatttgc tacaattgcc aatacaaggt ttgttgccct ggtagatgga
     4561 atggatatca gcgctccact tttaaatttt ccaacaggca acctggtagg aattcctgat
     4621 ttagatgttg aaagtgttga attggttccc ggagctgcat cggctcttta tggacccaat
     4681 gctttcaatg gaattctttt tatgaatagt aaaaaccctt ttgaatacca gggattaagt
     4741 gcccaggcaa aactcggcgt gactgaatcc aatgccaacg gaactaatcc catgtacaac
     4801 tttcaactcc ggtatgcaaa agcctttaat aacaaggttg cctttaaggt aaatttctcc
     4861 atcatggaag ctacagactg gagagccaat gactatacca ctgacaaaaa ttttcccatc
     4921 agggctcctg gagattccaa tttcgatggc ctgaatttat atggtgatga agttgagatt
     4981 ttctatggtg gcctcggtcc tttcctggga gcgcttgact taaggagaac cggatggaag
     5041 gaggaggatt tacttgaaac agatgcagcg agaagtataa aggcagatgc cgctttgcat
     5101 tacagattaa ctgataaaat tgaagcgctt tataactacc gctttggcgg cggtcgctct
     5161 atctatcagg gatccggcaa gtttattctc cgggatttta cccaacagtt tcacaaattg
     5221 gaactgaaat cagataattt cttcgtcaga ggctacatga ctcaaacaga tgatggtgat
     5281 tcctataata tggatgccct ggggatctac ctcaatgaat caataagtcc gacagcaacc
     5341 gagtgggctc caatttatat tccggcgtat gtacttgcgc tacaaggata ttataccgca
     5401 cttggagtac ctgcgggtaa taagcaagct gcacacgcgt ttgccagaca aacagcagac
     5461 gccaccagac ctgcggttgg aagttcacag tttactaacc tggtcgagac aatcaggact
     5521 acaaaattta atgaaggcgc gggagccgca tttgttgaag gatcccggtt atatcatgga
     5581 gagttcaatt acgatttcaa aaatatgatt gacattgttg atatccagat aggtggtaat
     5641 ttccgccagt atgacatttt tagcgatgga actatttttg atgaggaaca gaatgcagac
     5701 ggatcttatg aaagagtgaa gataaatgag tatggcggat atgtgcaact tggtaaatct
     5761 attggcaatc taaaactggc cgcttccgcg agatatgata agaatgaaaa ttttgaagga
     5821 cagtttaacc ccagagtatc cgcggtttat accgcaggcg gatctcataa tttcagagca
     5881 tcattccaaa caggtttcag aaatcctgac tcacagtcac aatttatatg gttcccaact
     5941 gcatccggca ttttaattgg aagtacagaa gccaatgcag cccgatatgg attgcataat
     6001 gggggtgcat ataccaacag gtcagtacta tctttctttg atgctggtgg atcatttgac
     6061 acagatggga ctccgctgga tgcagacgga aatccaactg atccagtttc cgcaggttta
     6121 gcaacagata attttgacta cgttcaaccc gaaaaattac aggcatttga agtgggctat
     6181 aaaggaatca tcggcggcga cttgttaatt gatttgaatt attaccacaa tatttataat
     6241 gactttattg cccaaaaaac cgtatacaac aaattaccca taatgaggcg aggtcaggtt
     6301 gtaacgggag ctaccggaga tattattact gagttcagac cctacctgaa tgcgcaggaa
     6361 aaagttacct ctgatggaat tggtttagga ttcacttaca acctgggacg cggatttaat
     6421 ttcaacggtt cctatgatta tgccaccttt aatgtgagta aagttgacct taattctgaa
     6481 ttcgaagcag gctttaatac tccggaacat aagtttatgg tgggattcgc caatagagat
     6541 gttggtgaaa acattgggtt tagtgtagcg ttccgatggc aggatgaatt cctatggcaa
     6601 tccgcctttg gtgagggaac aattccttcg tatggaaccc ttgattttca gataaataag
     6661 aaactgaaag cactgaaatc agttattaaa gttggcgcta caaatcttgg cggccaggat
     6721 tatcgaacca atctcggtgc cggatttgtt ggcactatct attatatctc tttaacattt
     6781 gaccaattcc tgaattaaaa agtttaatta actatgaaaa cgaacatatt tagatatctg
     6841 gttttagcta ttctgattgg aggttgcaat caggaatttg atgaggtaat tcccgaccgg
     6901 acagatttgg gttctggcca cgaaacattc tccgccggac ctcttgactt taccactttt
     6961 gttgctatcg gaaactcgct aacagcaggc taccaggccg gtgcgctatt tacggaaggt
     7021 caaagcaatt ccctgccgct tattctttcg aaacaatttg caagagtagg tgggggaacc
     7081 tttaaccagc cgacaataaa tacagtgaat gggtattccg gcatgagtgg atctacagtt
     7141 tttggaaggc ttttgttaca gctggttggt aatcctccaa cacccgtacc aacaccacaa
     7201 ctatctgatg gtggtgcgat tcccaatatt ctggtaaatc cgtcattcgc ttattcaggg
     7261 aacaaagcta ctcttaacaa ttttgcggtt ccgggcatcc aggtgggcca ggcattaatt
     7321 cctgaaaccg gagactggaa taattccggc catccggcct tcaatcccta ttacgccaga
     7381 tttgcatcgg cacccggcac ttctacgatc cttggcgatg cgctgacccg caatcccacg
     7441 ttcttcttct tctggctggg caacaatgat attctgggtt atgctgttgg cggtgcaagc
     7501 aacgaggcta tctttacctc cacagataat ttcacagcct attacggagc cgctttgggt
     7561 gcattaaccg ctaaccctgc cgtggaaggt attgtcggaa atattcctaa tgtaacttct
     7621 attcctttct tcaaactggt tccatggaat gctattccac ttgatactga caaggcaaca
     7681 gcattgaata ctaacccgaa tttccaggat ttgaacggcg cagttacagc aggtaacaac
     7741 ttaggattta tttcggatgc ggaggctgca tccagaatgt taagttggca agccggacaa
     7801 aatggagccc ttataattga tgaagatttg tttgacctga gaaatttgga tccaaatatc
     7861 ccggaattgt atgcaaaagt ccggcttgcc aatagcactg atttactgcc gctaactgca
     7921 ggtactgttt taggtgttta ccttgttcct ggaaacgatg ccactattca aggtgtttca
     7981 taccctgttg gggatcaatt tgccctcact gcaagtgaac aaacagcaat tgccgatcgc
     8041 accattgcat ttaacaatat tattaaatcc atattggatg gaattaacgc cggtggcaaa
     8101 agggtagctt tatttgatgc taacgccagg cttgccggcc ttaatactgt tccgacttat
     8161 atcagcaatg ttatggttga tgctacgctt gctccaccat ttggtgcatt ttcagaagat
     8221 ggagttcacc caaacagccg gggttatgct ctggccgcat attggattat tcagcaaata
     8281 aatcaggagt ttggatccaa tgttcccagg ccccaaattt cggaatacca gggaaccgga
     8341 ttaccaataa attgaaaacc taacgggagg tcataaccaa tgacctcttt ttttttggaa
     8401 aagcaaacgt attattacct attgacaggc tggattctaa ttgcccttct ctcctacccg
     8461 tttctgagaa ggaaaaccca accctacggc cgacat
//
LOCUS       DASWHX010000138        12142 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_1623037, whole genome shotgun sequence.
ACCESSION   DASWHX010000138 DASWHX010000000
VERSION     DASWHX010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 12142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12142
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1623037"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="VGA21_09400"
     CDS             complement(<1..237)
                     /locus_tag="VGA21_09400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650766.1"
                     /translation="MKHKRSPSKLKIKLKIIKEDEGYTAVGQWRNRSLVTCGDTWDEL
                     QTMILEMVNLSFEDLGYTFTIEEIQFEYDLESFFD"
     gene            361..2778
                     /locus_tag="VGA21_09405"
     CDS             361..2778
                     /locus_tag="VGA21_09405"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX9650767.1"
                     /translation="MGIIFNFLKTSRRILLKRKVFFFIILSCLTVGFSAFLSLYNFIS
                     FEYSYDQFHKNSDKIYRVQLERKYTDRVDLTAGVTAGVAPFMKEEFPEVQSFVKLWGT
                     SHMRNIIRHGSLSIESEKLYFASEDFFRIFTFPLVSGNPETALANPNTAVITKSLAKK
                     IFGENNPLGKTFIYHNSWEATNVEITGIVEDVPVNSHLKFDMLVSFKTLERFIPSAAD
                     SFGWSAFNTYLLMNGTELPPDFQNRVDQFISTHYAPLIEQGIKVNLYFQPLSEVYLGP
                     EIRFQPETGGSREILQTLTIAAIFILLLAWLNYINLSTASVRDRALEVGVRKVAGASK
                     KDLVMQFLFEAFILNLLAIIISLMVVELSHSLLNTILGKQIPGEIYLRPDTWLISGIL
                     VLGGTLLSGFYPAIALASFKPLSVLKGSHGGSKFLGLRKGLVAFQLLVSFMLISGTLV
                     IYHQIRFMQQKNLGVSLDKVVAIKGPGLLDSAWTQKLFYLKQQISGLSDIQMVANTTS
                     IPGIEITWVNNDVRWLGNPDDNRYSLPFTGISSEYLDLMNISLLKGRNFVDGMEAENE
                     NVLLTASAAKVLGFTTPEAAIGERVTDSNIEYTVVGVTSDFHQQSLNKRISPVIFRYV
                     PNANSYLVVRLNTGSISETIGFLEQEWSEVFTGNPFDYFFLDEFFENQYSSDKRFVNI
                     FGIFTIIGLFIGGLGLLALSLFMVRRRESEMSIRKVMGATDLQIMKLFTSEIFTILGI
                     AVVLGVPSSYYLSDRLLQNYPYKIPFQFTWVIAPFGILLAILVITSAWRIIQISMKSP
                     VEAIREQ"
     gene            3164..3904
                     /locus_tag="VGA21_09410"
     CDS             3164..3904
                     /locus_tag="VGA21_09410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650768.1"
                     /translation="MNSIFINNIIIVFFFFMNVQANYLFSQQLSENSFDRKLIYDEKK
                     FYLSIVLSHSVLSEGSPLKSVFSKYNLDQKYSFLGFGTKYPWYEDYLNLTADINYPLT
                     SSFSLGIKSGLNSWGRVAGYGNGDFILLRYMNFVLGSFIELNGKAGECYKLGTTLNYH
                     RIINTHDDVKNKKINTIKPGIYFGFVFPLRNSTRFGWNLTFDYYYGGNVPIGPYESST
                     QGTIMAPRTKIAAHNIAIGNLFRWRLSR"
     gene            complement(4397..4855)
                     /locus_tag="VGA21_09415"
     CDS             complement(4397..4855)
                     /locus_tag="VGA21_09415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650769.1"
                     /translation="MKMNRPRFIEILFITCVAFLFPILNAHAQETNANLKDIQLIKSG
                     DFKHLAGMIEFPRAKNENRAFFNKGMPDQQLFFLQLDTPIKLPKGAKEGEIYYNKSMA
                     VFSSEHSNEIIVFVLDDQSIQESDFEGYPVKILRGFGIGCYTGDQITRGD"
     gene            5117..6184
                     /gene="corA"
                     /locus_tag="VGA21_09420"
     CDS             5117..6184
                     /gene="corA"
                     /locus_tag="VGA21_09420"
                     /inference="COORDINATES: protein motif:HMM:TIGR00383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HEX9650770.1"
                     /translation="MARFLEKQEGKFGKDPGDLTFIGKQRLEVPIIEVFDYSQNHFEK
                     VTFQNLDELSKLKDSDSVSWINIDGLHDIELMQKIGQIFDLHPLLLEDIMNTSQRPKF
                     EEFDNCIFIVLKMLWLIKDSDSVENEHTSFVVGKNFLISFQEGLPGDVFNPIRDRLQR
                     ATTKVRNRRPDYLAYALLDVIIDNYIIIIERFGERIELLEGKLSSNPNEELLQEINNY
                     KTEINFLRKTIRPVRELAVSFEKSDSEIIEDENRPYIKSLVDHVAHSAEAIETYQIML
                     NDQLNVYQTKQSNKLNEILRVLTVFSVIFIPLTFIAGVYGTNFEHFPELEFQYGYYVF
                     WAAMIFTALVMVYFFKRRKWL"
     gene            6547..7536
                     /gene="gap"
                     /locus_tag="VGA21_09425"
     CDS             6547..7536
                     /gene="gap"
                     /locus_tag="VGA21_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009582333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEX9650771.1"
                     /translation="MTKIAINGFGRIGRLTFKALLNKPGIEIVAINDLTEPSILAHLL
                     KYDSVHGRFNGEVVAQESALVVNGKTIKILKERDPRNLPWAKDGVKLVLESTGLFVDE
                     AGAGGHIQAGAKQVVISAPAKGNIPTVVLGVNDDTLKGDEPIISNASCTTNCLAPMAK
                     VLDDNFGIEKGYISTIHAYTSDQRLQDAPHKDLRRARAAAYSIIPTSTGAAKAVGLVL
                     PHLKGKLDGIAMRVPIPDGSLTDLTAIVKKEVTAEEINAVMKSAASGAMKGILEYTED
                     PIVSIDIIGNPHSCIFDAGLTSTNGTLVKVVGWYDNEAGYSNRTADLIAKLLG"
     gene            complement(7542..8288)
                     /locus_tag="VGA21_09430"
     CDS             complement(7542..8288)
                     /locus_tag="VGA21_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002702983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX9650772.1"
                     /translation="MSGHSKWANIRRRKEAQDSKRGKIFTKLIKEIAIAARIGGVDMD
                     ANPRLRLAVQNAKGVNMPKDNIQRAINKGSGSDGTEYSEITYEGYGPGGVAIFVETTT
                     DNVNRTVSNVRSAFSKYGGSLGTNGSLEFIFQRKGIFTYPVPVDFIEDEFTLEVIDAG
                     AEEVEIEDGYVTVTTAMEDFGRMQKKLDGLQIEVEEAELQRIPNTLVKVEDEIFSKVI
                     KLIETLDDDDDVQKVYHNLDASTEQFESIS"
     gene            complement(8329..9183)
                     /locus_tag="VGA21_09435"
     CDS             complement(8329..9183)
                     /locus_tag="VGA21_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017730841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX9650773.1"
                     /translation="MQITKGVRYMLVSTLFFTGMNLLVKMVPNIPAIEIVFFRSLVSL
                     VLSYGFLARLRISVWGNNKKLLIGRGISGAVALILYFTTLQVMPLASAVTIQFLSPIF
                     TTILGIFVVREKVKPWQWAFFAIAFGGVLVIEGFDPRVSAFFGVIGIISALFSGFTYN
                     LIRKMGTSEHPLVIVFYFPLVTLPVTGIYCYFFWVNPIGMEWLLLILIGVMTQFAQYF
                     MTMAYQNEELSKVAPLKYFGVLYAIILGYVFFGETFNWLTYTGMIMVIVGVILNVWYK
                     QRLTSRVI"
     gene            9256..9705
                     /locus_tag="VGA21_09440"
     CDS             9256..9705
                     /locus_tag="VGA21_09440"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009195743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEX9650774.1"
                     /translation="MALLEGTKAPDFTLKSTTGSMFSLHKDQLGSRCILYFYPKDFTP
                     GCEKEACDFRDQFFHFEELNVAIYGISRDSYETHLKFREKFVLPFHLLSDPDGKVSKK
                     YDASVPLLKISKRITYFIDEELKIAAVYDNFFGAHLHMKRVLERINR"
     gene            complement(9695..10345)
                     /locus_tag="VGA21_09445"
     CDS             complement(9695..10345)
                     /locus_tag="VGA21_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020533894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9650775.1"
                     /translation="MLEALSITKSFGTLPVLKGINLKVAKGEIVSIVGASGAGKSTLL
                     HILGTLDKPDKGEVKLNGVNLETLQGNSLAEFRNKHVGFIFQFHNLLPEFTALENVCI
                     PGFIGKRDDREVTDRAKELLELLGISERRDHKPSEMSGGEQQRVSVARALINNPQIIY
                     ADEPSGNLDSHNADELHKLFFDLKKKLHQTFVIVTHNQELAEMADRKLTIVDGIIG"
     gene            10475..11665
                     /gene="sucC"
                     /locus_tag="VGA21_09450"
     CDS             10475..11665
                     /gene="sucC"
                     /locus_tag="VGA21_09450"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010610485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HEX9650776.1"
                     /translation="MNIHEYQAKEVLSGYGVRIQRGIVASNIEQAVEAAKQLNTETGT
                     KWWVIKAQIHAGGRGKGGGVKLAKSLDDVKTLSDQIIGMQLVTHQTGPEGKKVHKVLI
                     TEDVYYPGESSPKEFYLSILLDRAKACDVIMASTEGGMDIETVAAKTPEKIIKEWIDP
                     RVGLQGFQARKVAFKLGVEGEAFKEMVKFIGALYKAYVGTDASLFEINPVLKTSDNKI
                     MAVDAKVNLDDNALYRHRDLAELRDLTEEDPLEVEAGAAGLNYVKLDGNVGCMVNGAG
                     LAMATMDIIKLSGGEPANFLDVGGGASPKTVEAGFRIILKDPNVKAILINIFGGIVRC
                     DRVASGVVEAYKNVGNIDIPIIVRLQGTNAEEGAKIIEESGLKVTSAILLKDAAQKVK
                     EALS"
     gene            11672..11745
                     /locus_tag="VGA21_09455"
     tRNA            11672..11745
                     /locus_tag="VGA21_09455"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11706..11708,aa:Arg,seq:tcg)
     gene            11875..>12142
                     /locus_tag="VGA21_09460"
     CDS             11875..>12142
                     /locus_tag="VGA21_09460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650777.1"
                     /translation="MKKMKSLTASLLFLFYISCTSESTDYIILSPGNLNSIEFFISKD
                     GTANYLVKHNNEIVIDTSSLGFEFKNQPPLQNGLTIIGSSTQTIN"
BASE COUNT         3679 a         2394 c         2355 g         3714 t
ORIGIN      
        1 gtcaaagaag ctttcaaggt catattcaaa ttgtatttcc tcaatggtaa aggtatagcc
       61 caaatcttca aatgaaagat tcaccatttc caggatcatc gtttgaagtt catcccaggt
      121 gtctccgcaa gtaacaaggc ttcggtttct ccattgacct accgctgtat atccctcatc
      181 ttccttaatg attttcagct tgattttaag tttggaaggg gaccgtttgt gcttcatttt
      241 tagatcctgg attgcactga aatgaaacat aatatcattt ctaaaaagtg taactttcta
      301 ctggatagtt agttataaaa atggtattga atttgttttc ctggatgaaa ataaataagt
      361 atgggtataa tctttaactt tctaaagaca tcacggagaa ttcttcttaa gagaaaagtt
      421 ttcttcttta ttattttatc ctgcctcact gttgggtttt cagcattttt atcattgtac
      481 aattttatat catttgaata tagttatgat caattccaca agaactcgga caaaatctac
      541 agggttcagt tagagcgcaa gtatacagac agggttgatc tcacagccgg tgtcacggca
      601 ggtgtggctc cctttatgaa ggaggaattt ccggaagtac agtcctttgt taagctttgg
      661 ggcacttctc acatgcgcaa tattattcga catggatcgc tcagcataga gtcggaaaag
      721 ctatacttcg ctagtgaaga tttctttcgt attttcacct tcccattggt atccggcaat
      781 ccggagactg cattagcaaa tccaaacact gcggttatta cgaagtcgtt ggcaaagaag
      841 atttttggtg agaacaatcc attggggaaa acatttatct accataattc ctgggaagca
      901 accaacgttg agataaccgg cattgttgaa gatgttccgg taaacagcca cctgaaattc
      961 gatatgcttg tttcattcaa aacgttggaa aggtttatac cttcggcagc agattcattt
     1021 gggtggagtg catttaacac ctatttgcta atgaatggaa ctgaattgcc tccagacttt
     1081 caaaacagag ttgatcaatt tattagcaca cactatgccc cattgataga gcagggaatt
     1141 aaagtaaacc tgtattttca accattgtcg gaagtttacc tgggaccgga aatacgattc
     1201 cagccagaaa caggtggaag tcgggaaata ttacaaacgc tcacgattgc ggcaattttt
     1261 atcttattgc tagcgtggct gaattacata aatctatcta ccgccagtgt tcgtgacaga
     1321 gccttggaag tgggagtacg caaagttgca ggagcatcaa agaaagattt ggttatgcaa
     1381 ttcttatttg aggcttttat tctgaatctg cttgccatta tcatttcgct aatggttgtt
     1441 gaattgagtc attctttgtt aaatacgata ttaggaaagc agattcctgg tgagatttat
     1501 cttcgtccgg atacatggct catttcaggt atccttgttc ttgggggaac attgctatca
     1561 ggcttttacc cagctattgc attggcatca ttcaaaccgc taagtgtttt aaaaggaagc
     1621 catggaggtt caaaattttt gggactgcga aaaggtttag tcgcatttca gttgctggtt
     1681 tcattcatgc ttatatcggg aacgctggta atctatcatc aaatccggtt tatgcagcaa
     1741 aagaatttgg gtgtaagtct ggataaagta gttgcaatta aagggccggg gttactggat
     1801 tccgcctgga cccaaaagct gttttattta aagcagcaaa tttccggatt gtctgatatt
     1861 cagatggtgg caaatacgac atcaattccc ggcatcgaga taacatgggt taataatgat
     1921 gtgcgctggc ttgggaaccc ggatgataac cggtattccc tgccctttac aggcataagt
     1981 tcagaatacc tggatctgat gaacatttct ttgctcaaag gccggaattt cgttgacgga
     2041 atggaagcgg aaaatgagaa tgtgcttcta acagcaagtg cagcaaaagt tctgggtttt
     2101 acaactccgg aagcggcaat tggagaaaga gtgacggata gcaatattga gtataccgtt
     2161 gttggcgtaa catccgattt tcatcagcag tctttgaaca aacgaatttc gcctgtaatc
     2221 ttccggtatg ttcccaacgc aaattcatac ctggtcgtga gattaaatac cggatcaata
     2281 tccgaaacca ttggttttct ggaacaggaa tggagcgagg tttttaccgg aaatccattt
     2341 gattattttt tcctggatga attttttgag aatcaatatt caagtgataa acgatttgtg
     2401 aacatttttg gaatcttcac tattattgga ttattcatcg gagggctggg tttgttagca
     2461 ttgtcgttgt ttatggttcg caggagggaa tccgaaatga gtatccgaaa agtgatggga
     2521 gcaaccgact tgcaaattat gaaactattt acttcggaga tttttactat cctgggtatc
     2581 gcagttgttt taggagtacc gtcatcctac tatctctctg atcggcttct gcaaaattac
     2641 ccttacaaaa tcccgtttca atttacctgg gtgatagctc cttttgggat attacttgcc
     2701 atcctggtta tcacctccgc atggcggatt attcagattt ctatgaagag tccggtggag
     2761 gccatacggg aacaatagtt tcaattagta acaagcaatt tacagttggt ttgaaatacc
     2821 ctgtttcagt acccttttaa ctttcattat tccctttctt gtcgagtcat caagtcacgc
     2881 tgtttatccc tcaggtagtg catttaattc gataaagcca tctctggagt caattcctaa
     2941 gataattagt gattgccgtt taagtgaagt cactaacaac ttttgcaaaa aagatttcga
     3001 taaattgcaa aatttaattt taaccacgtt aaccctatga ttacatttat atgagaatgg
     3061 ttttaaataa ttgttatgtt ctgcggaata aagaacttct tttaatcaga ttcggagcag
     3121 aaaacataca atcattaaaa cagaaaaggt aacaccaaat aaaatgaatt caattttcat
     3181 aaataatata atcatcgtgt ttttcttctt tatgaatgtt caagctaact atctattttc
     3241 acaacaattg tcagaaaatt cgttcgatag aaaattaatt tatgatgaga agaaatttta
     3301 cctgtccatt gttttatctc atagtgtttt gtcggagggc agccctttaa aaagtgtttt
     3361 ttccaaatac aatcttgacc agaaatattc tttcttgggt ttcggcacca aatacccctg
     3421 gtacgaagat tatcttaacc tgactgcaga tattaattat cccttaactt cttcattctc
     3481 attgggaatt aaatccggtc ttaatagttg gggaagagta gcagggtacg gaaatggtga
     3541 ttttattctt ctcagatata tgaattttgt gctgggttca tttatagagt taaatggaaa
     3601 agcgggagaa tgttataagc ttggaactac gctcaactat cacagaatta tcaacacaca
     3661 tgacgacgtg aagaataaaa aaatcaatac aataaaaccg gggatatact tcggatttgt
     3721 ttttccactc cgcaactcaa ctcgtttcgg ctggaatctc acctttgatt attattacgg
     3781 aggcaatgtg cccatcggtc cttatgaatc ttccacgcag ggtactataa tggctccacg
     3841 aacaaaaatt gccgcacata atattgcaat aggaaattta tttagatgga ggctttcccg
     3901 ttaactaaga ttttcatgat ctctgaaaat ttcaattcct gacacttgac tgctagggat
     3961 tgtcttttgc aagtagatca ctgatttgtt tgattctagg acacttgctc aaaagtggtc
     4021 aactctatct aggacacctc aaattgtaaa ttgataattg taaattacta attgaaacca
     4081 tgacagcccc caaccacatc atcggaggct agtatttact gaattttctg ctcatttttg
     4141 gcccgaattc ttcgcgcatt aataacttta cctttaaata aagtacatta tgtctgctcc
     4201 gaatcatgtt ataggcggag tagtctttac cggaatattc tgctcctttt ggaacatcaa
     4261 catttcctca agtccattat acctgtcttt acaatcttca catcattact cccagatgta
     4321 gatcacaccc gttgcataat tacagataat ggaatttgta tctaattcga ttcgtggatt
     4381 tgggcaacta ttattttcaa tctccacgtg taatttgatc accagtataa catccaattc
     4441 cgaatcccct aagtattttg actggataac cctcaaaatc actttcttgg atactttgat
     4501 catcgaggac aaatactata atttcatttg aatgttctga actaaaaact gccatgcttt
     4561 tattatagta aatttcaccc tcctttgcac ctttaggcaa ttttatggga gtatccagtt
     4621 gaagaaagaa tagttgctga tctggcatgc ccttgttaaa gaaagctctg ttttcgtttt
     4681 tcgcccgggg aaattcgatc atgcctgcta aatgcttaaa atccccggat ttaatgagtt
     4741 gtatgtcctt taaatttgcg ttggtttctt gtgcatgggc attcaaaata gggaataaaa
     4801 atgccacaca agtaatgaac aaaatttcga tgaaacgtgg tctattcatt ttcacttctt
     4861 tacaatctaa ggaaaattgg aaacctaata agcaggaatt taaccattta tgtccaaaca
     4921 ccctcctttc tcctcttgaa tttgaattgc aacagaaaaa gatgaaattt aatcttaaat
     4981 atatgttcaa caatgcctca aaaacgatca actctatcta ggacacctca aattgtaaat
     5041 tgtaaattgt aaattgctaa aatggtgact tttattacat ttcctcgtct aaggattcgt
     5101 atagataaag aactaaatgg caagatttct cgaaaagcag gaagggaagt ttggtaaaga
     5161 tccgggcgac ctcacattta taggtaaaca aaggctggaa gtgccaatta ttgaggtttt
     5221 tgattactct caaaatcact tcgaaaaagt aacgtttcag aatctggatg agctttcaaa
     5281 acttaaagac tccgattcag tttcctggat taatattgat ggactgcatg atattgagtt
     5341 aatgcagaaa atcgggcaaa ttttcgatct ccatccgttg ttattggaag acatcatgaa
     5401 taccagtcaa aggcccaaat ttgaagagtt tgacaattgc attttcatcg ttctcaaaat
     5461 gctgtggcta atcaaagatt ctgactcggt tgagaacgaa cataccagct ttgtggtggg
     5521 caagaatttt cttatttctt tccaggaggg tttgcccggt gatgtgttca atcccattcg
     5581 cgacagactc cagcgggcaa caacaaaagt aagaaaccgc agaccagatt atctggctta
     5641 tgccctgctt gatgtaatca ttgataatta catcatcata attgagcgtt tcggtgaaag
     5701 gatcgaattg ctggaaggaa aactttcatc gaatcctaac gaggaacttc ttcaggaaat
     5761 caacaattat aagactgaaa taaatttcct caggaaaaca atcagaccgg ttagagagtt
     5821 ggccgtctca tttgagaaat cagattcaga aattattgaa gacgaaaacc ggccttatat
     5881 caaaagtctt gttgatcacg ttgcacattc agcagaagcc atagaaacct accaaatcat
     5941 gcttaacgat cagttgaatg tctatcaaac caaacaatca aataaactca atgaaatact
     6001 cagagtattg acggtttttt ccgtaatctt tattcctctt acctttattg ccggggtata
     6061 tggaaccaac tttgaacatt ttcctgagtt agaatttcaa tatggatatt acgttttctg
     6121 ggcggcaatg atttttaccg cgctggtgat ggtctatttt ttcaaaagaa gaaaatggct
     6181 ttaaataccg gttgtttcct agtggagtct tttacatgtg ccacactttg ccaacattga
     6241 aaatcaatca attttcattt aaaataaaaa gtggactgtg acacacgttg atgttgattt
     6301 catttggatt taaactgaga tacaacctaa atcgctggtt ccttaactta ttttgtgcaa
     6361 ataatttaag aattatggat atttaacgcg tagctccaat ttttcggagc attcaatttc
     6421 cttactatta atcggcatta ttccttatta tcaaaagaat tcattctttt aatcttcatc
     6481 ctttgttggt aataaaatgt gtccgaagta gttttgcttg catcaatcca aaatcatcaa
     6541 caaagcatga ctaaaattgc cataaacgga ttcggaagaa taggaagatt aacctttaaa
     6601 gccttattga ataaacccgg tattgaaatt gttgccatca acgatttgac agaaccatcc
     6661 atcctcgccc atctattaaa gtatgattca gtgcacggaa gattcaatgg tgaggttgtc
     6721 gctcaggaat cagccctggt agttaatggg aaaactatca agattctgaa agaaagagat
     6781 cccaggaatt taccctgggc aaaggatggt gtgaaactgg tattggaatc aaccgggtta
     6841 tttgtggacg aagccggcgc aggcggtcat attcaggccg gtgcaaagca ggtagtaatt
     6901 tctgcacctg ccaaaggcaa tattccaacg gtcgttctcg gagttaatga tgatacctta
     6961 aaaggcgatg aacctataat atccaatgct tcctgcacaa caaactgtct tgcccctatg
     7021 gcaaaagttt tggatgataa tttcggaatt gaaaaaggat atatctccac cattcatgcc
     7081 tatacttctg accaaagatt gcaagatgct cctcacaagg accttcgcag agcaagagcc
     7141 gcggcatact caataatccc aacttctacg ggcgctgcaa aagctgtggg actggttctt
     7201 ccgcacctaa aaggtaagct ggatggaatt gccatgcgag ttcctatccc cgatggttca
     7261 ttaaccgacc ttacggcaat tgtaaaaaag gaagttactg ctgaagagat taacgcggtg
     7321 atgaaatccg cggcatccgg tgccatgaaa ggaattctgg agtacacgga agatccaatt
     7381 gtttccatag acattattgg aaatcctcat agttgcatct tcgatgccgg tttaacatcg
     7441 accaatggaa cgcttgtaaa agtggtgggt tggtatgaca acgaggcggg gtattcaaac
     7501 cgtactgcag acttaatcgc gaaattatta ggttaacctt ttcagcttat tgactcgaat
     7561 tgttctgtgg aagcgtccag gttatggtaa accttttgga catcatcatc atcatccagg
     7621 gtttcaatta acttgataac cttggaaaaa atctcatctt caacttttac aagagtattt
     7681 ggtattcttt gaagttcggc ttcctcaacc tcgatttgca gtccatccaa tttcttttgc
     7741 attcttccga aatcttccat tgcagtagta acagttacat agccatcctc aatttcgacc
     7801 tcttcggcac cggcatcaat cacttccaga gtaaattcat cttcaataaa atccacagga
     7861 accgggtaag taaaaatacc tttcctttgg aagatgaatt caagagagcc atttgtacct
     7921 aaacttccgc catatttgct aaatgctgaa cgaacattgg aaactgtccg gtttacatta
     7981 tcggttgttg tctccacaaa aatagcaaca ccgcccggtc catatccttc ataagtaatt
     8041 tctgaatatt cagttccgtc agaccctgaa cctttattaa ttgctctttg aatattgtct
     8101 ttgggcatat ttactccctt ggcattttga accgccagcc ggagccgggg gtttgcatcc
     8161 atatcaactc cgccaatcct tgccgctatg gcgatctctt ttatcaactt tgtaaagatc
     8221 tttccgcgct ttgaatcctg ggcttccttt cttctcctga tatttgccca tttgctatgt
     8281 cctgacattc ttctttttct tcaaaattaa ttcaaccttt taactctttc aaattaccct
     8341 ggaagttaac ctttgcttat accaaacatt gagaattact ccaacaatta ccatgatcat
     8401 ccccgtatag gttagccaat taaaggtttc gccaaaaaat acatacccta agataatagc
     8461 atataatact ccgaaatact taagcggcgc aactttagaa agctcctcat tttggtaagc
     8521 catcgtcata aaatattgtg caaactgagt cattacacca attaaaatca aaagtaacca
     8581 ttccatccca atgggattaa cccaaaagaa gtagcaatag ataccggtta ccggcaaggt
     8641 taccaatgga aaataaaaaa caattaccag gggatgctcc gatgtgccca ttttgcggat
     8701 caaattatat gtaaatcccg agaataaggc agaaattatt cctattactc cgaaaaatgc
     8761 cgaaaccctg ggatcaaaac cttcaattac taaaactcca ccgaatgcaa tagcaaaaaa
     8821 tgcccactgc cagggcttta ctttttctct aactacaaag attcccagta tggttgtgaa
     8881 aatgggagat agaaattgaa tagttaccgc gctggcaaga ggcattactt gcaatgttgt
     8941 aaaataaagt atgagggcaa cggctcctga tattccccgt ccaatcaaaa gctttttatt
     9001 atttccccaa acacttatcc ttaatcgggc tagaaaacca taactgagga caagtgaaac
     9061 cagtgatctg aagaaaacaa tttcgatagc cggaatattg ggaaccattt tcacaagcaa
     9121 gttcattcct gtaaaaaaca gtgtagaaac caacatgtac ctgactccct ttgtgatttg
     9181 caatagctga agaattgtgt ggggcaaagg tattaatcca gcttaattta ttatgtttaa
     9241 tcggaaatta atataatggc attgctggaa ggaactaaag cacctgactt caccttgaaa
     9301 tcaaccacag gatctatgtt ctctttgcat aaagatcaat tgggcagccg ctgcattctt
     9361 tacttttacc caaaagattt tactcccggt tgtgaaaaag aggcttgcga tttcagggat
     9421 caatttttcc attttgagga actgaatgtt gctatttatg gaatcagccg ggattcttat
     9481 gagactcacc tgaaatttcg tgagaaattt gttttacctt ttcaccttct ttctgatcct
     9541 gatggaaaag tttccaaaaa gtatgatgca tccgttccgc tgttaaaaat ctcgaagaga
     9601 attacttatt ttattgacga agaattgaaa attgccgctg tttatgacaa ctttttcgga
     9661 gcacatctgc acatgaaacg ggtactggaa agaattaacc gataattcca tccacaattg
     9721 ttaatttcct atcggccatt tcggcgagtt cctgattatg agtaacaatt acgaaagttt
     9781 gatgcaactt tttcttcaaa tcaaaaaaca atttatgcag ttcatccgcg ttgtgagaat
     9841 ccaggttacc actgggttca tccgcatata tgatttgagg attatttatt aatgcccttg
     9901 ccaccgacac ccgttgttgc tcccctcctg acatctccga tggtttgtga tcccttcttt
     9961 cggaaatgcc cagcaattcc agtaactctt tcgccctatc cgtcacttcc ctatcatctc
    10021 gctttccaat aaaacccggt atacacacat tttcaagggc ggtgaattcc ggcaacagat
    10081 tatgaaactg aaaaataaac ccgacatgtt tatttctgaa ctcagccaat gaatttccct
    10141 gcaaagtttc caggtttacc ccgtttaact taacctcacc cttgtcaggc ttatccagcg
    10201 ttcccaggat gtgcagcaag gtacttttac ctgcacctga tgctcctaca atggacacaa
    10261 tttcaccttt cgcaaccttt aaattaattc cctttaagac gggtaaggtg ccaaatgatt
    10321 tagttatgga aagtgcttca agcatttggc caaagataac caaccgcctc ttagtaataa
    10381 agatggcgaa gaaattaagc tctgaacgtc ccttttctgc cttgaattat atacccaaaa
    10441 aacgtagatt cgtgcaaaat ttaaatccca gaatatgaat atccacgaat accaggcaaa
    10501 agaggtctta tcaggctatg gggtccgaat acagcgggga attgtggcct ccaacattga
    10561 gcaggcagtg gaagcggcaa agcagctaaa cactgaaacc ggcacaaaat ggtgggtgat
    10621 taaagctcag attcatgcgg gtggaagagg taaaggtggt ggagtaaaac ttgccaaaag
    10681 tctggatgat gtgaaaactt tatctgatca gattatagga atgcagttgg taacccatca
    10741 gaccggacct gaaggaaaga aagttcacaa agttttaatt actgaagatg tttactatcc
    10801 gggggaatct tcaccaaaag aattttacct gagcatttta cttgatcgcg caaaagcgtg
    10861 cgatgtcatt atggcctcaa cggagggtgg aatggatatt gaaacggttg ccgccaagac
    10921 acccgaaaaa ataataaaag aatggataga cccgcgagtc ggacttcagg gattccaggc
    10981 aaggaaggtt gcatttaagc tgggagttga aggagaagca tttaaagaaa tggttaaatt
    11041 tataggtgca ctatacaaag cttacgtcgg aacggatgca tcattgtttg aaatcaatcc
    11101 tgtattaaag acctcagata ataaaatcat ggcggtggac gcaaaagtta atcttgatga
    11161 taacgcgtta taccggcaca gggatttggc tgagctaagg gatttgacag aagaagatcc
    11221 attggaagtt gaagcaggtg ccgccggact taattatgtc aagcttgatg gaaatgttgg
    11281 ctgcatggtt aacggtgccg gtcttgctat ggcgaccatg gatattataa aactttccgg
    11341 tggggaaccg gctaactttc tggatgttgg cgggggagca tctcccaaaa ccgttgaggc
    11401 agggttcaga attattttga aagatcctaa tgttaaagca attctgataa acatttttgg
    11461 aggaattgta cgctgcgatc gtgtcgccag cggagttgtg gaagcatata aaaatgtcgg
    11521 caatattgat atcccgatca ttgttcgttt gcaggggacg aatgccgaag aaggcgcaaa
    11581 aattattgaa gaaagtggcc taaaggttac ctctgctatt ctgcttaaag atgccgcgca
    11641 aaaagttaaa gaagcgttgt cataacattt tgcatccgta gctcaactgg atagagcatc
    11701 agacttcgga tctgagggtt gagggttcga ctccttccgg gtgcacttca ttcatcttca
    11761 ttttttaagg attcttatca gaaaacaaaa acgggattat taactggaac atgaatctcc
    11821 cgacgatgtg ttattgcatt caaaataagt catccaattg taataacttt cactatgaaa
    11881 aagatgaaat ccctcaccgc atcccttttg ttcctttttt atatttcctg cacttcggaa
    11941 tcaaccgatt acattatttt atctcccgga aacttaaatt ctattgagtt ctttatttct
    12001 aaagatggaa ctgcaaatta tttagtcaaa cacaacaatg aaattgtcat tgatacttcg
    12061 tcgttaggct ttgagttcaa aaatcaacct ccactgcaaa atggcctgac cattatagga
    12121 tcttcaaccc aaactattaa tg
//
LOCUS       DASWHX010000139         5499 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_510743, whole genome shotgun sequence.
ACCESSION   DASWHX010000139 DASWHX010000000
VERSION     DASWHX010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 5499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5499
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_510743"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1452
                     /locus_tag="VGA21_09465"
     CDS             <1..1452
                     /locus_tag="VGA21_09465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018341681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX9650778.1"
                     /translation="ILTLDLESMEYKPKSRVKFDTLEKTKPIENIRERFKILLGGKDK
                     AGEFYRSSFYALFQYASNRIPEIADELYKIDDAVCAGFGWELGPFETWDSANLQKSLP
                     KMEEAGYKPNQWVYDMITAGAESFYTVQDGVRKYWDINSKQYLPLPGKDEFIILDNIR
                     ENKKVWGNAGTTLFDVGDGILNLEFHTKMNTMGEDVIRGVHHAIDTAEKDFRGLVIGN
                     QGTNFSAGANLGVLFMLAIEQEYDEINFSIKHFQDTMMRVRYSSIPVVVAPHALSLGG
                     GCEMTLHGDRVQAAAETYIGLVEVGVGLIPAGGGTKELTKRVSDGLQEGDVELNALQN
                     AFMNIATAKVATSAHEAFDMNILRRGDGITLNKDRQIADAKKLALELADAGYVQPVPA
                     SNIKVQGRTGMALFYAGINGMKMGNYITEHDMKIARKIANVMCGGDLSYPQEVSEQYL
                     LDLEREAFLSLTGEKKTLERIQAILQGQKPLRN"
     gene            1460..2635
                     /locus_tag="VGA21_09470"
     CDS             1460..2635
                     /locus_tag="VGA21_09470"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020527979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEX9650779.1"
                     /translation="MDAYIVKGFRTAVGKAKKGGFRFYRPDDLAADVIKHLLNSIEGF
                     DPMQVDDLIVGNAVQEAEQGMQMGRMISLLALPKEVPGMVINRYCGSGLEAISIASTK
                     IHAGLADCIIAGGTESMSLVPVMGYKTALNYKIASENPTYYTSMGLTAEQVSIDFNIS
                     REDQDEFSFNSHRKAAAAIKEGKFKDEIVPVTVKENYVDASGKKKTREFVVEVDEGVR
                     EDTSIEALSKLKPAFAIGGVVTAGNSSQTSDGASFVIVMSEKMMRQYNLQPIAKLISY
                     AAAGVDPRIMGIGPVKAVPKALDKAGLKLSDVDQIELNEAFAAQSLAVIRELGMDMEK
                     VNVNGGAIALGHPLGCSGAKLSVQLFNEMRRRNQKYGMVTACVGGGQGVAGIYELLN"
     gene            2650..4449
                     /locus_tag="VGA21_09475"
     CDS             2650..4449
                     /locus_tag="VGA21_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015334292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX9650780.1"
                     /translation="METLDKKSAIKGGEFLVKEITADEIFIPEEWNEEQLMIRQTCYD
                     FVKQEVWPKLDELDSMKNPELMPKLLERAGELGLLGTALPEQYGGFGTDFNTTMLIAE
                     ALGAGHSFAVAFGAHTGIGTLPIFYYGTEAQRNKYLPKLANAQWKAAYCLTEPDSGSD
                     ANSGKTKATLSPDGKHYLINGQKMWITNGGFADLFIVFAKIDNDENLSAFIVEKSFGG
                     ITMNEEEKKMGIKGSSTRQIFFNDCKVPAENLLSERGNGFKIAVNILNIGRIKLGISC
                     VGGAKETLSMAIQYANERKQFGKSIGSFGAIKQKLADMAAYIYAAESAGYRASQNIDD
                     AVEAYKASGVDEIEAKIKALYQFAIESAIMKILGSEALDFTVDEGVQIYGGMGYSAEA
                     PMDRAYRDARINRIFEGTNEINRMLTVDMILKRTMKGELDLMGPAMTVALELTSIPDF
                     SQEVEEGIFVKEKKLLANFKKIGLMIAGAAVQKYMQKLGDEQEILMNLADMLVEGYAA
                     ESTILRVEKLMKLRGEENCQIQKEMAMIYLYRALDKISNAGREAIYSFAEGDEMRMML
                     LGLKRFTKAEPYNLKNSRRKVADYLLKENKYAL"
     gene            4458..4967
                     /locus_tag="VGA21_09480"
     CDS             4458..4967
                     /locus_tag="VGA21_09480"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX9650781.1"
                     /translation="MARPEKSEYSPFYQPYIDRVEQSELITALQHSFRTLMEFPKSIP
                     PEKYEFRYNEGKWTVKEVFIHLADTERVMQYRALRFSRNDKAQLHAFDENDFIKNSNS
                     KNLNFEEVYEDLINVRKSSLTFFKSCSEAMLLHSGIANNANVTVRALGFIIAGHQAHH
                     FNVIHTKYF"
     gene            5041..5235
                     /locus_tag="VGA21_09485"
     CDS             5041..5235
                     /locus_tag="VGA21_09485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEX9650782.1"
                     /translation="MKLTAIIEKSEDGWFVGQIEEIPAAISQGKTIEELKRNLLDALQ
                     LILESNRAETEVIREELVLV"
BASE COUNT         1789 a          989 c         1315 g         1406 t
ORIGIN      
        1 atactcacat tggatttgga atccatggag tataaaccga agtccagggt aaagtttgac
       61 acgctggaaa aaacaaaacc aatagaaaat attagggaaa gatttaagat acttctgggg
      121 gggaaagata aagcgggtga attctacagg agttcttttt acgcattgtt tcaatatgcc
      181 tcgaacagga tcccggaaat tgcggatgaa ctttacaaga ttgatgatgc cgtttgtgcc
      241 ggttttggct gggaattagg accattcgaa acctgggata gtgcaaatct tcaaaaatcc
      301 ttacctaaaa tggaagaggc cgggtacaaa ccaaatcaat gggtttacga catgattacc
      361 gccggtgctg aatcatttta tacagttcag gacggggttc gaaaatattg ggacattaac
      421 tcgaaacaat atctgccatt accaggcaaa gacgaattca ttattctgga taacatcagg
      481 gaaaataaaa aggtttgggg taatgcagga accacacttt ttgatgttgg tgatggaatt
      541 ctgaaccttg aatttcacac taagatgaat acgatggggg aagatgtaat aagaggcgtc
      601 catcatgcta tagacacggc agaaaaagat ttcaggggat tagtaatcgg aaatcagggt
      661 accaatttct cagccggcgc aaatttggga gttttgttta tgcttgccat tgaacaagag
      721 tatgatgaga tcaacttctc tattaaacat tttcaggata ccatgatgcg ggttcgatac
      781 tcatcaattc ctgttgttgt tgcaccgcat gcattatcac ttggtggcgg atgcgaaatg
      841 actttacatg gagacagagt acaggctgca gcagaaacct acatcggtct ggttgaagtt
      901 ggagtaggat tgatccccgc aggtggcggt acaaaggagt tgacaaagcg agtttcggat
      961 ggcctgcaag aaggagatgt tgaactgaat gctttacaga atgcattcat gaatattgca
     1021 acggcaaaag tagcgacttc agctcatgaa gcatttgata tgaacatcct gcgcaggggc
     1081 gatggcatca ctctgaacaa ggaccggcaa atcgccgatg caaaaaaact ggcattggaa
     1141 ttagctgatg caggatatgt acaaccggtc ccggcttcaa atattaaagt tcagggaaga
     1201 acgggtatgg cattgttcta tgcagggata aacgggatga agatgggcaa ctacattact
     1261 gagcatgaca tgaaaattgc caggaaaatt gcaaatgtta tgtgcggcgg agatttatct
     1321 tatccacagg aagtaagcga acaatatctc ctggatttgg aacgggaagc atttctttca
     1381 cttacaggtg aaaagaaaac attagaaagg attcaggcaa tattgcaggg acaaaaacca
     1441 ttgagaaact agtacagcta tggatgcata tatcgtaaaa ggatttagaa ccgcggttgg
     1501 gaaagccaag aaaggtggct ttagattcta ccgacccgat gatcttgccg ctgatgttat
     1561 taaacatctg ttaaattcca ttgaaggatt tgatccgatg caggttgatg acctgattgt
     1621 tggcaatgca gtacaggaag cggaacaagg aatgcaaatg ggccgcatga tcagcttact
     1681 ggcattaccg aaggaagttc ccggcatggt tataaacagg tattgcggaa gcggacttga
     1741 agctataagt atcgcttcga caaaaattca tgctggactt gctgactgca tcattgccgg
     1801 cggaaccgaa tccatgtcac ttgtaccggt tatgggctat aaaactgcac tcaattataa
     1861 gattgcttct gaaaacccaa cctattatac cagcatgggt ttgaccgcgg aacaagtatc
     1921 aatagatttt aatatttcca gggaagacca ggatgaattt tcattcaact cccacaggaa
     1981 agcagctgcg gcaattaaag aaggaaaatt caaagatgaa attgtaccgg taacggtgaa
     2041 ggaaaattat gtggacgctt ccggaaagaa aaagacccgg gaattcgtgg ttgaagttga
     2101 tgaaggagtt cgggaggaca cctcaataga agctctgtca aaattaaaac ctgccttcgc
     2161 cataggcgga gttgttaccg cgggcaattc atcccaaacc tctgatggcg cttcatttgt
     2221 catagtgatg tcggaaaaga tgatgcggca atataatctt caacccatag caaaactaat
     2281 aagttatgcg gcagccggcg ttgatcccag aattatggga atcggaccgg taaaagcagt
     2341 gccaaaagct ctggataagg caggcttaaa acttagtgat gtcgatcaaa tagaattgaa
     2401 tgaggccttt gcagcgcaat cattggcagt tataagagag ttaggtatgg acatggagaa
     2461 agttaatgta aacgggggag cgattgcgct gggacatccg ctaggttgct caggcgccaa
     2521 actgtctgtt cagctcttta acgaaatgcg aagaagaaat caaaaatatg gaatggtcac
     2581 cgcatgcgtt ggaggaggac aaggcgttgc cggtatttat gaattattga attaaaattt
     2641 attttgaaga tggaaacgtt agacaaaaaa tcagcgatta aaggcggaga atttttggta
     2701 aaggaaatta ccgcggatga aatatttatt ccggaagaat ggaatgaaga acaattgatg
     2761 attcggcaga cttgctatga ttttgtgaag caggaagtat ggccgaagtt ggatgaattg
     2821 gattcgatga agaacccgga actcatgcca aaacttttag aacgcgctgg cgaattggga
     2881 ctgttgggca ctgctttgcc cgagcaatac ggtggattcg gcacagactt caacaccaca
     2941 atgttaattg cggaagcttt gggcgccggt cattcatttg ccgtggcttt tggtgcacat
     3001 accggcattg gcacattgcc aattttttat tatggaacag aagctcaaag aaataaatac
     3061 ctgccaaagt tagcgaatgc tcaatggaag gcagcttatt gcctaacgga accggattca
     3121 ggatctgacg ctaattcagg aaaaacgaaa gcaacattat cccccgatgg gaaacattac
     3181 ctgatcaatg gacaaaaaat gtggattacc aatggagggt ttgccgatct cttcattgtt
     3241 tttgccaaaa ttgataacga tgaaaacctt tctgccttta tagtagaaaa atcatttggt
     3301 ggcattacca tgaatgaaga agaaaagaaa atgggaatta aaggatcatc aactcgtcag
     3361 attttcttta atgactgcaa agtacctgca gaaaatttat tgtctgaacg aggtaatggc
     3421 tttaagatcg cggtaaatat tctgaacatt ggtcggatta aacttggaat ttcctgtgta
     3481 ggtggtgcca aagaaacatt aagcatggct atccaatatg ccaatgaacg aaagcaattc
     3541 ggaaaatcta ttggcagttt cggcgccata aaacaaaaac tcgcggatat ggctgcttac
     3601 atttacgcgg cggaatcggc aggttaccgt gcatcgcaaa acattgatga tgccgttgaa
     3661 gcatacaaag catcaggcgt ggatgaaatt gaggccaaaa taaaagcctt atatcagttc
     3721 gctatcgaat ctgccattat gaaaattttg ggttccgagg cactcgattt tactgtagat
     3781 gagggtgtac agatttatgg cgggatgggc tactctgccg aagcacccat ggaccgggcc
     3841 taccgggatg cgcgcatcaa ccggattttt gaaggtacca acgaaatcaa ccggatgctg
     3901 acggtggata tgattctaaa acgaacaatg aagggagaac ttgatttgat gggaccagcg
     3961 atgacggttg ctctggaact gacatcaata ccggatttta gtcaggaagt ggaagaaggt
     4021 atctttgtaa aagagaagaa actactggca aattttaaga agatcggatt gatgatagca
     4081 ggggctgctg ttcagaagta tatgcaaaag cttggtgatg aacaggaaat tctgatgaat
     4141 cttgctgata tgctggtgga gggatatgca gcggaatcca caatactgag agttgaaaaa
     4201 ttaatgaaat taagaggtga agaaaattgt cagatccaaa aagaaatggc catgatttat
     4261 ctttatcgcg ccttagataa aatctcaaat gccggcaggg aggcaattta tagttttgcc
     4321 gaaggcgatg aaatgcgaat gatgctcctg ggtctgaaac gatttaccaa agcggaaccc
     4381 tataacctga aaaactcccg tagaaaagtt gcggattatt tgttaaaaga aaacaaatac
     4441 gcgttgtaag tttccggatg gctagacctg aaaaatccga gtattcgcca ttctaccaac
     4501 cttatattga tcgggttgaa caaagcgaat tgattaccgc gctgcaacac tcattcagga
     4561 ccttaatgga atttccaaaa tcaattcctc cggaaaaata tgaattcaga tacaacgaag
     4621 gaaagtggac tgtgaaagaa gtatttattc acctggcgga tacggaaaga gtgatgcaat
     4681 accgggcgct gcggttttca agaaatgaca aggcccaact tcatgcgttt gatgaaaacg
     4741 acttcatcaa aaacagtaat tcaaaaaatt taaattttga agaagtttat gaagatctga
     4801 tcaacgttag aaaaagcagt cttacatttt tcaaatcatg ttccgaagcc atgcttttac
     4861 attcgggaat tgctaacaat gccaatgtaa ctgtcagagc cctgggtttc attattgccg
     4921 gccaccaagc tcaccatttc aatgtgattc acacaaagta tttctgatca ggcgtcagac
     4981 ggttccaaac cgtcggacgc ctctgctttt caattcacac caaattatta tctttataac
     5041 atgaaactta cagcgataat cgaaaaatca gaagatggct ggttcgtcgg ccaaattgaa
     5101 gagatacctg cggctatttc tcagggcaaa accattgaag aacttaaaag aaatttgctg
     5161 gatgcattac aattaatttt agaaagcaat agagcagaaa cagaagttat tcgtgaagaa
     5221 ctggttttgg tgtgaagcgc aaaaaactga ttaaacattt gaggaacgaa ggatgtcagt
     5281 tattaaggga gggaggaaat cactccatcc atattaatcc tgaaaacaaa agacaatcct
     5341 cagttggaag gcattaggaa ttatctgatt tattatgcag aaaaatttgc aaacagctgg
     5401 gtataaaaat ttttaagttc tctgatatga aacgtcttcc atgctactca tcatactctt
     5461 atggacattc tgatcaggtc gtcagacggt tccaaaccg
//
LOCUS       DASWHX010000140         5788 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_2003007, whole genome shotgun sequence.
ACCESSION   DASWHX010000140 DASWHX010000000
VERSION     DASWHX010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 5788)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5788)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5788
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2003007"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..718
                     /locus_tag="VGA21_09490"
     CDS             <1..718
                     /locus_tag="VGA21_09490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650783.1"
                     /translation="VVILFIFLYQPGLLRAEIKNGYEIELEETRQSLENINTMIKMEG
                     RNNRERMIKLKKYLIKLKKIEARQSIYSEKTRKLLLRLKEIDPTLFSEIDSIKDGKGN
                     STDVYVKVVPLNKDQEIHGSTNLDQYNFDEDMYSSNFGTKTTSVIVADCFRSIFLLAH
                     ELGHVKYQVPNLAEYFNFYFKTYRNSVQSKKGHARADQSGMSADETVKSFTQLYVSYR
                     QSKNSKAATISSVKQKPDEY"
     gene            complement(918..1805)
                     /locus_tag="VGA21_09495"
     CDS             complement(918..1805)
                     /locus_tag="VGA21_09495"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX9650784.1"
                     /translation="MLTYYFFRATILLLCLIPFKFLYIFSDFVFILLYFIFGYRKKIV
                     LENVNNSFPELSSKERIAIVKKFYNNLSDILMEGLKGFTMNQIQFQKRYKLINPELLN
                     DYFDLGLDIICVGSHYANWEWGIMAAPRQVKHQMTAIYTPLTNKRINKYTIKNRSRYG
                     TGMIPKSEARKALSEKRRIPTAYLFGGDQSPARIRNVHWMELLNQDTAVLKGAEHFAK
                     LLKCPVIYFDVQRVKRGYYTVGVSTLFQNPCETSEGEITEKYMRTLEEIVRRKPEDFL
                     WSHRRWKHKREREEVTTQE"
     gene            complement(1888..3204)
                     /locus_tag="VGA21_09500"
     CDS             complement(1888..3204)
                     /locus_tag="VGA21_09500"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX9650785.1"
                     /translation="MLKKIFSCFSFLLISTGLVFSQETFDLPGLVDRILSENYQIRIL
                     RNEEIINENNNTLGNAGFLPVVSLSGGRSNTVNNTKQKLFNGDIREGKNAENTSLNGL
                     IRVDWNIFNGFGMFARRDQLSVLETISQEETRYLVEQTITDVAGLYYQLVRGRLRLEN
                     FEKTLAVSRFRHDLEKKKLGVGASYALLYNQAFIDYNSDSLLVIDQLRANKSLEIRIN
                     RIIRRNPDLQLETINELELEEFDNLGSLVTGATTHNRDLKLADLNELIAETNLKIQKS
                     DMYPTLDVFAQYAYNRSTNEIGVTESNRSYGAQYGFTVRFNLYNGRNERREIRNAQIV
                     TQNTTFAREDISAEIEAIVLDLFFQHESLTRQLSIAEQNVSAAEKSQFIAREQLEKQA
                     ISGYDFRLTQLSVINAFNTVTEIKYNLKSLEIELNRLTGRLAEKYL"
     gene            complement(3185..>5788)
                     /locus_tag="VGA21_09505"
     CDS             complement(3185..>5788)
                     /locus_tag="VGA21_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020530312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX9650786.1"
                     /translation="NNVFKERLQTIPGVSSIRVWGERKYAMRIILNPDKLEAFSLTPL
                     DIRRALETQNVELPAGRIEGQKTEITVRALGRLNFPEEYNEMVITKRNNSLVRLKDIG
                     YAAIGSENERTRMYGNGVLPMIGVALQPQPGANHIDIVDEAVRRVDQIKNDLPEDVQL
                     NVALDTTISIRKAITEVKETIFIAFGLVLLVIFFFLRSWRVTMIPVTVIPISLIGVFS
                     VLYLAGYSINILTLLGLVLATGLVVDDAIVVMENIYSKVEKGHDPVQAAFKGSREVFF
                     AVIATSISLVCVFLPIFFLEGLTGRLFREFAMVVSGAIVISTFVSLSLTPMMSSRILK
                     KSKRKGKLMMSFKAVVDFFTQKYNKSLRIFMKQRWLAIPIVLILAVLIVTLNMIIPKE
                     LAPLEDKSRIRMSISAPEGTSFESMDEFERLMMHMSDTIRGKEFLIGLTAPGFGSAGS
                     VNSGFVRISLTQPSQRDRSQMEIAQQLTDLIKKYNFAQTFVIQEPTIIASRGGRNSLP
                     VQFVLQAPDLDRLKEVLPKFLERVENDPTFQASTVDLRFNKPEYTVNINRNKALDMGV
                     NVSDIAETLQTYLSDQRIGYFIRDGKQYFVIAQAERESRDEPLDLARIPIRNAAGDMI
                     TLDNLVTLQLESRPPQLVRYNRYVAATVSAAPSEGKTIGDGVDAMRRIAGEVLDDSFQ
                     TSLAGSASDYAESSSNITMIFIFALILVYLTLAGQFESFRDPLTIMFTVPLALAGALM
                     TLYFFGQTLNIFSQIGIIVLVGIVTKNGILIVEFANQKRAEGMTKIEAAIEAASQRFR
                     PIVMTSLSTTLGALPIALAMGDASTSRIPMGISIIGGLLFSLILTLFIIPSIYTYISG
                     KPKKVYVEENI"
BASE COUNT         1726 a         1250 c         1060 g         1752 t
ORIGIN      
        1 agttgttatt ttattcattt ttctttatca gcccgggttg cttagggcgg aaatcaaaaa
       61 tggctatgaa attgaattgg aggaaacacg ccagagtctg gaaaatatta acactatgat
      121 taaaatggaa ggcaggaata atcgtgagcg gatgataaaa ctgaaaaagt atctaatcaa
      181 actcaaaaaa attgaagcca gacagagtat atattctgaa aagacccgga aattactact
      241 tcgattaaaa gaaatagatc cgacattatt tagtgaaatt gattctatca aagatggaaa
      301 agggaatagt acggatgtat atgttaaagt tgtgccgtta aataaagatc aggaaattca
      361 tgggagtact aaccttgacc aatacaattt tgatgaagac atgtattcct ccaattttgg
      421 aacaaaaacc acaagtgtaa tcgtggccga ttgtttccgc agtatttttc ttcttgctca
      481 tgaactgggg cacgtaaaat atcaggtgcc gaacctggca gaatatttca atttctattt
      541 caaaacctac cggaattcag ttcaatctaa aaagggccat gccagggcag accaaagcgg
      601 aatgtcggcc gatgaaaccg ttaaatcttt tacacagtta tatgtgagct atagacaatc
      661 aaaaaattcc aaagcggcaa caatttcttc agtaaaacaa aaacctgatg agtattagtt
      721 tctgtaatga ccagtgtcat tgaaacggca gtcttctcct gagttttaca gaaatttaga
      781 ttgcattcct tccacgaatt cagattatac aggacatgac aactgctgcc agcagtaagt
      841 gtctcaaagt ttaatttgac taaaaataag gcaaatcctt tatttttccc cttcctaaat
      901 tccaccggaa ttcaaaatta ctcctgggta gttacctcct cacgttcccg cttatgcttc
      961 caccttcgat gagaccacaa aaagtcttcg ggcttcctgc gaacaatctc ctccagggta
     1021 cgcatatatt tttcagtaat ctctccttca gaagtctcgc aaggattctg aaacaatgtg
     1081 gaaaccccca ctgtataata ccctcgttta acccgctgca catcaaaata aattaccgga
     1141 cattttaaaa gttttgcaaa gtgctccgcg ccttttagca ctgcggtatc ctgattcaat
     1201 aattccatcc aatgaacatt cctgattcgt gccggacttt gatcaccacc aaataaataa
     1261 gcagtgggaa ttcttctttt ctctgacaat gcttttctgg cttcagattt aggtatcatt
     1321 cccgtaccgt accgggatcg attcttaatc gtgtatttat tgattcgctt gttagttaac
     1381 ggagtgtaaa tggctgtcat ctggtgttta acctggcgtg gagctgccat aattccccat
     1441 tcccaatttg catagtgcga tcccacgcaa ataatatcca gtcccaaatc aaaataatca
     1501 ttaagcaact ccggattaat tagtttatat cttttttgaa actgaatttg attcatggtg
     1561 aagcccttca atccctccat aaggatgtca cttaaattgt tgtaaaactt ttttacaata
     1621 gcaatcctct ccttgctact taattccgga aaagaattat tgacattttc aagcacaatt
     1681 tttttacgat aaccaaaaat gaaatacaaa agaataaata caaaatcaga gaaaatatag
     1741 agaaacttaa agggaatcag gcaaagcaat agtattgtgg ctcgaaaaaa ataataagta
     1801 agcatttgat gtactgggtt tggtagggtg taagtaagat aaagactctc aaatatgcta
     1861 taaatttttt gaacggtgga agaaccttta caaatacttc tccgccaacc ttccggttaa
     1921 cctgttaagt tcaatctcca gagatttcaa attatactta atctcagtaa ctgtgttgaa
     1981 tgcattgata actgaaagtt gagtaaggcg aaaatcatat cctgaaatag cctgcttctc
     2041 cagttgttca cgggctatga attgagattt ttcagctgcg gaaacatttt gctccgcgat
     2101 agataattgc cttgttaacg attcgtgctg gaaaaacaaa tccagcacga tggcttcaat
     2161 ttcagctgaa atatcctccc tggcaaaggt agtattttga gtaactattt gtgcattgcg
     2221 tatttcccgg cgttcatttc tgccattgta taaattgaat cgtactgtaa atccatattg
     2281 tgcgccgtag ctccggtttg attcggttac tccaatttca ttggttgacc ggttatacgc
     2341 atactgtgca aacacatcta atgttgggta catatcagat ttttgaatct ttaaattagt
     2401 ttcagcaatc aattcgttta agtccgccaa cttcagatcg cgattgtgag ttgtcgcccc
     2461 ggtgaccagt gatcccagat tatcaaattc ctctaactcc aactcattaa ttgtttctaa
     2521 ctgtaaatca ggatttcttc taataatccg gttgattcga atctccaggc ttttattggc
     2581 tctcagctga tcgataacca gaaggctgtc agagttgtaa tctataaatg cctgattata
     2641 tagtagcgcg tagcttgcgc ctactcccag ttttttcttt tccaggtcat gtctgaatct
     2701 ggataccgcc agggtctttt caaaattctc caatcttagt ctgccgcgaa ctaattggta
     2761 gtataagccg gctacatcag taatggtttg ctcgacaagg tatcgcgttt cttcctgact
     2821 tatggtttcc aatacactca attgatcccg cctggcgaac atcccgaatc cattgaagat
     2881 attccaatca actcttatca atccattaag gctggtgttt tccgcatttt tcccttcacg
     2941 aatatctcca ttaaagagtt tttgttttgt attattgaca gtgttagatc gtcctccgga
     3001 taaagacact acaggcaaaa atccggcatt gcccagcgta ttattatttt cattaattat
     3061 ttcttcattt cttagaatgc gaatctggta attttcggaa agtattctgt ctaccaagcc
     3121 tggcagatca aaagtctctt gcgaaaacac cagaccggtc gaaataagga gaaaactgaa
     3181 acaactaaat attttcttca acataaactt ttttcggttt acctgatata taggtataga
     3241 ttgaaggaat tataaacaaa gtgaggatta atgaaaacaa caaaccacct atgatagaaa
     3301 tacccatggg tattctgctt gttgaagcgt ctcccatggc cagggcaata ggtaatgcgc
     3361 ctaaagtggt tgagagactt gtcattacaa taggtctgaa cctttgtgat gcagcttcaa
     3421 tggctgcttc aatttttgtc attccttcag ctcttttctg atttgcaaac tccacaatca
     3481 gaattccgtt ttttgtaact atgccaacta aaacaataat tccaatctgg ctgaaaatgt
     3541 ttaaagtttg accaaaaaag tatagagtca tcagggctcc agccaaagcc aggggcacgg
     3601 tgaacattat ggttaaagga tctctaaaac tctcaaactg cccggcaaga gtaaggtaaa
     3661 ccagtataag cgcaaaaata aatatcatag tgatgttact ggagctttcg gcgtaatccg
     3721 aggcacttcc ggccagggat gtctggaatg aatcatccag tacctcaccc gcaattcttc
     3781 gcattgcgtc cacaccgtca ccaattgttt ttccttcgga aggtgccgcg gaaactgttg
     3841 ctgccacgta gcggttgtag cgaaccaatt gagggggtct gctttccaat tgcaatgtta
     3901 ccaggttgtc cagggtgatc atatcacctg ccgcatttct gatgggtatt cttgccaggt
     3961 ccaaaggctc atccctgctt tccctttccg cctgagcaat cacaaaatac tgtttgccat
     4021 ccctaatgaa ataacctatc cgctgatcac taagatacgt ttgaagggtt tccgcaatat
     4081 cgcttacatt aacacccata tccagcgcct tgtttctgtt gatattaact gtgtattcag
     4141 gcttattaaa tctgagatcc acggttgaag cctgaaatgt tggatcgttt tcaacccgct
     4201 ctaaaaattt tggaagtact tcttttagcc gatctaaatc gggagcctga agcacaaatt
     4261 gcacgggcaa actatttctt cctccgcggc tggcaataat agtaggctcc tgaattacaa
     4321 atgtctgagc aaaattatat ttcttgatca aatcagtaag ttgctgagct atctccattt
     4381 gacttcgatc acgctgcgac ggctgcgtta aggaaattct gacgaatcct gaatttaccg
     4441 atcctgcgga tccgaatccg ggtgcggtaa gtccgattag aaattccttt ccccttatgg
     4501 tgtcactcat atgcatcata agtctttcaa attcatccat actttcaaat gatgttccct
     4561 ccggagcaga aatactcatt ctgattctgc ttttatcttc aagaggagcc agttctttgg
     4621 gtattatcat gttcagcgta acaatcaaaa ccgccaaaat caatacaatt ggaatagcta
     4681 accacctttg cttcatgaag attctgagcg acttattata tttctgggta aaaaagtcga
     4741 ctaccgcctt aaatgacatc atcagttttc cttttcgttt gctttttttc agaattcttg
     4801 atgacatcat cggggttaga gacaaggaaa caaacgttga aataacaatt gctccggaaa
     4861 ccaccatggc aaactctcta aaaagccttc cagttaatcc ttccaggaag aagatgggta
     4921 gaaatacgca aacaaggctt attgaagttg caattacagc aaagaaaact tctcgtgaac
     4981 ccttaaaggc cgcctgaacg gggtcgtgtc ccttttcaac cttcgaataa atattctcca
     5041 tcactacaat agcatcatcc accaccaggc cggttgccaa aacaagtccc agcagggtga
     5101 gaatgtttat agaataacct gccagataca acactgaaaa cacaccgatt aaggaaattg
     5161 ggataacagt taccggaatc atagtgacgc gccagcttcg caggaagaaa aagattacca
     5221 gcaataccaa accaaaggcg ataaatatgg tttcttttac ctcggtgatg gcttttctta
     5281 tactgatagt tgtatctaac gccacattaa gttggacatc ctcaggaaga tcattcttaa
     5341 tttgatcaac ccttcggacc gcttcatcca caatatcaat atggtttgct ccaggctgtg
     5401 gttgtaaggc tactccaatc attggcaata cgccgttccc atacatccgg gttctttcat
     5461 tttctgatcc tatagcagca tagccaatat ctttaagtct aaccaatgaa ttattccgtt
     5521 tagttattac catctcatta tactcctctg gaaaattcaa ccttcccaaa gcccggactg
     5581 ttatttcagt cttctgccct tctattctgc cagcgggtag ttctacattt tgggtctcca
     5641 aagcccttcg aatatccaat ggcgttaagc tgaaagcttc cagcttatca ggattaagga
     5701 taatgcgcat agcatatttt ctttcacccc aaacacgaat actgctgact cccggaatgg
     5761 tttgaagcct ttctttaaac acattatt
//
LOCUS       DASWHX010000141        20804 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_514968, whole genome shotgun sequence.
ACCESSION   DASWHX010000141 DASWHX010000000
VERSION     DASWHX010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 20804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20804
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_514968"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            156..228
                     /locus_tag="VGA21_09510"
     tRNA            156..228
                     /locus_tag="VGA21_09510"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:189..191,aa:Thr,seq:cgt)
     gene            251..323
                     /locus_tag="VGA21_09515"
     tRNA            251..323
                     /locus_tag="VGA21_09515"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:284..286,aa:Gly,seq:ccc)
     gene            417..2714
                     /locus_tag="VGA21_09520"
     CDS             417..2714
                     /locus_tag="VGA21_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019598670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_function: GO:0003997 - acyl-CoA oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEX9650787.1"
                     /translation="MSSSLLEKNPRLYIFLPLLYMVWEDAVLTPSEIKTMQDLIEKQD
                     WLTSEEIDFLSGYLNPSQPPSPQELKEWLEEFSKIKLSADTKTLSEIGIELAKKNGAV
                     NGNEIDEKIKSDIMEIENVLGIISRESIFNFYPEKRKTFTASQKTEQTFTVEKLAEIL
                     DGSQRGLIKKIKLILSDPEFRYFHGDNLQARREKVLEWSRLLAKQGFGALAYPGEYGG
                     KNDMEAYFTVMETLSYHDLSLVIKFGVQFGLWGMSVYLLGTEKHHKKYLKKIGTLELP
                     GCFAMTETNHGSNVKGIETTATYHHTTKTITINTPHHYARKNYIGNAACHGQAATVFA
                     KLIIEGKDYGVNAFVVPLRDEAGNTLSGITIEDCGRKMGLNGVDNGIIHFDNVQIPLD
                     EMLNRFATINEHGVFESLISSDNRRFFTMLGTLVGGRIGIPRAGLSAAKSGLTIAIRY
                     ADQRLQFGPEGATEVPILNYRTHQRRLMPLIANAYALHFALQYLTKRFLNRTEEEMQE
                     VEALAAGLKAFATWNTTATLQECRECCGGKGYMTENRIDDLKNDTDIFTTFEGDNTVL
                     MQLVAKSRLTEFKQEFGSMNLFGILNYVIDQAATSFMELNPIIVRKTDAGHLLDPEFH
                     LDAFIYRERDILTSAARRLRKHLDNGMDSFDAFNQTQYHMVNVGFAYVERIILEQFQV
                     AVKNTKDKGCKNILQKLCNLFALSQLEKNKGWYLEHGYMEGVKTKAIRKLINQLVWEI
                     RQEAVPLVNAFEIPDSCLSAPIAIR"
     gene            2819..3712
                     /locus_tag="VGA21_09525"
     CDS             2819..3712
                     /locus_tag="VGA21_09525"
                     /inference="COORDINATES: protein motif:HMM:TIGR03709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006797 - polyphosphate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PPK2 family polyphosphate kinase"
                     /protein_id="HEX9650788.1"
                     /translation="MRNQKHEFDHSVFIVPPGKKTKLSNFDPDYVGQIKGKKHSKETL
                     REDIDDLASAQELLWASAEKSMLIIFQALDAAGKDGTIKHVMTGINPQGCNVYSFKAP
                     TEEERLHHFLWRPNRFLPPRGRIAIFNRSYYEEVLIVRVHPEILKSAWLPREMRKMSP
                     EKIWKTRFNEIRDTEKRWTQNGIQIVKFFLNVGKSEQKKRFMERLNNSDKNWKFSVND
                     FKERQYWDGYMKAYEEMLNETSTSYSPWYVVPANNKWYTRAVVADVITKRISEMDLAF
                     PAVSESTMAELREVKKKLMEE"
     gene            complement(3760..4989)
                     /locus_tag="VGA21_09530"
     CDS             complement(3760..4989)
                     /locus_tag="VGA21_09530"
                     /inference="COORDINATES: protein motif:HMM:NF027964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HEX9650789.1"
                     /translation="MRIIILGGTGRIGSAVAYDLVRNNAVNTVGVTGMGDLKLEKMAS
                     KLNSNKLNMHKVDLENQEAVTLLLRDYDAAIITLPNRRLSYKAIECAINAGTHAVDIL
                     EEYHRRPEPHETEGLEVPGDMSLEKYGESLHDRAMKSNVTILDGMGFAPGLSNITTQS
                     AIDYLDKAVSVIARVGGIPSRETSLRHPLKYLITWSFEHVLREYMVSLSIRKNGKVVQ
                     VQALEDHEIFEFTEFGKNDKLECFITPGMPSFIYTHPELDYFTEKTIRWPGHWQGIQT
                     LKECGLLETTQVEFQGSKISPREFMLAQLEPKLRPVKGDADACVMYNTIKGFKDNKEC
                     TVEFFMWDEEDKVNNITSMARVTGFSASIAGMMLAANAITQRGIVAPEEVISGELYDK
                     FLKELGKRNIKIAKKIN"
     gene            5242..8286
                     /locus_tag="VGA21_09535"
     CDS             5242..8286
                     /locus_tag="VGA21_09535"
                     /inference="COORDINATES: protein motif:HMM:NF013123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HEX9650790.1"
                     /translation="MKKAIILIATFLFLQFNVANAQWFGRNKPNYEQFNFQVKESPNF
                     SVYYYVKNDSLVESFIKSSEKWYKHHQKFFQDTLQERNPIILYGDHTDFQQTRIISDL
                     IGVGTGGVTEGLKNRVVMPVMELNSQFDHVLGHELVHVFQYNLLKTGDSISLNHIRNL
                     PLWMVEGMAEYLSIGSFDPNTAMWMRDAVLNDDIPTLKDLTQSNKYFPYRYGHAFWSF
                     VGGVFGDSVVVPLFRETALHGYQNAIKRISGLNEKEFSEAWSTHLKNYYKQFAPAHPD
                     STLEKDNVVGKLLIDPEKAGKLNIAPVISPNGQYVAFLSNMNLFTVDVHLAEIKTGKI
                     VKTLSSVVKNPQVDDFSYIESAGTWSPLSDRFAFAVLSRGKIKLSIVDMGRHKSLTME
                     IPGVHYFSNPTWSPDGESIVVSGLVNGQSDLFIFNIKTKDVRQLTEDFYSDIQPQWSN
                     DGQRLIFTSDRPVVGREYDMHALQICSLDLINKQITVLDIFPGSENLNPVFSPGDTSF
                     YFLSNQDGFRNLYQYDIDSRTTYKLTDYYTGISSLTPYSPSLSVSTKTGNLSYVYYGN
                     QTYQIYTASPEDFNKVPLVDGMANKNAGVLPPYNKQNIPQKINLKEDLSPPDSTIRDT
                     KYRPRFGLTYIGNSGGIGISTGTFGSTALSGGINMAFSDMLGYHNLYAAMAINGEIYD
                     AGGQVAYINQKHKINWGVTLSHIPYRTSSLGYKLDSLSVDNQNYLMENYFVDVYRMFQ
                     ESAGMFFYFPTSSTRRFELGSSYSYYSFRGERYNNYYYQAIYVNQQKEKIEAPGGYWL
                     GNTYAAYVFDNSHFGIASPQLGRRYRIEASQTYGELHFSTLTLDFRKYFFLNPTSVAF
                     RILHSGIYGGDSEKVYPMSFAYPGLTRGNSLDNIEVYGYNENKPYSINQIFGSRILVA
                     NAEWRVSFTGPERLALIKSQYFFSELALFVDAGMAWDSAHNPSFELKPNSTQDRVPFI
                     SAGLSYRINFFGQLILEPYYAFPWRGDKFLKGNFGINIVPGW"
     gene            complement(8313..8942)
                     /locus_tag="VGA21_09540"
     CDS             complement(8313..8942)
                     /locus_tag="VGA21_09540"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEX9650791.1"
                     /translation="MKIPQIFPKVWHLLFRSKAEVRMVNLISFYRIFSFPYLLLFIFT
                     GHLNIVKWLIAFSFFTDIVDGYLARKHNASSILGAKLDSIGDDLTILAGLIGVVVHFP
                     DFLLEEIWIIAAMLTLFIIQTILALKRYGKPTSFHTYIAKIAAFFQGFFFISLFFYGE
                     PVYWLFYGTAIVTTADLLEEIIIVVILPKWKSNVKGLYWVLKNKAELKL"
     gene            9385..10500
                     /locus_tag="VGA21_09545"
     CDS             9385..10500
                     /locus_tag="VGA21_09545"
                     /inference="COORDINATES: protein motif:HMM:NF037527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650792.1"
                     /translation="MTAIIDKLLDERGGLCLSLITTAHRTSPERRMGPILMKQSVEKA
                     VTLINTNYASHPDKNILIRNLHRMKETIDYNHLKEGLGLFISKDIHELIHFPFPVTDK
                     VEVDDRFLLRDLQYLRQVKKEYLVLSVGKKKLRAFRGNGEYLAEIINNDFPVIFHDDY
                     DYARSVRGTSFGLNAGKDFEKDKSIVKQIRMRDFYKSADQLVHNNLKGDQELVVYGDQ
                     KEVSAYLEITQDKDKVVDKVFSRYGAKGDTQLKSMAKIKIKNSQKESHKKLIEAIREF
                     PVQKVAIGLGNIWKAAYLGKGHQLVVEKDYTIHGYLSSDGSKLKLRRPRSKDSYVFIK
                     DAVENVLKLVKDLSGEIIFLDNDELKDLDRIALIKRY"
     gene            complement(10565..12817)
                     /locus_tag="VGA21_09550"
     CDS             complement(10565..12817)
                     /locus_tag="VGA21_09550"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX9650793.1"
                     /translation="MTFAKEKIVFMSTYPNRECGIATFCQDMVQALDTLFSENYNWQV
                     CALEENETDEYDYPDEVKYRLNTADKQQYMDFAVQLNKDRYVKGVCIQHEFGLFGGEY
                     GNDIFEFLNAVDIPVTIIFHSVLPAPNAELRKVVVALADSVERIIVMTDNSAGILNQD
                     YGIMQEKIRIIPHGTHHVSWSSPEALKKRYELGNKKIISTFGLISENKSIETALYALP
                     DIIKVFPDILYLVIGKTHPKIIERDGEKYRIFLEGLVKDLKLEKNVRFINRYLITPEL
                     LEYLQLSDLYLFTSKDPKQAVSGTFAYALSCGCPVITTPFPHARELLSNQVGYFFDFD
                     NHKQLAEAAIKLLQNPDDLKSKSFEAYRISQESVWQNVAIKLMKVFHELDKSAATWTI
                     NYNLPELRMSHFTNLVDDIGIVQFSKINVPDLSSGYTLDDNARALIAITMHYLKTGNS
                     NDLSLIRKFLDFIQLCQLDDGRFFNYLDSQGKYHYRNHYVNLNDSNGRAIWALGFLIS
                     KSDSLPPSISMDAKLSFTKSLLWLEEIDSPRSAAFIIKGLYYLYKHEPSKDLSEIIIK
                     LTDKLVGMYNSSNQFRWRWFENYLTYANAVMPEAVLMAYEITGYSKYLDVALNSFNFL
                     LSQTFKEDQIRVVSNRGWQLKGESKKSFGEQPIDITYTILALDSFHRILQLNNYEEYK
                     KIAFSWFLGNNKINRVMFDLNTGGCYDGLEETGANLNQGAESTVCFLISQMSMNIIDV
                     EKEEPVEHEV"
     gene            complement(12822..13865)
                     /locus_tag="VGA21_09555"
     CDS             complement(12822..13865)
                     /locus_tag="VGA21_09555"
                     /inference="COORDINATES: protein motif:HMM:NF015971.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650794.1"
                     /translation="MIEIKEKKILLEKTGNGFENDGVFNPAVIQDNGKIIIYYRAVRT
                     GNYSSIGYCELESPMVVKCRNERPLIFPEHDYESQGIEDPRITKVEDTYYLTYCAYDN
                     TNALGALAISKDLHHFEKKGIITPRFTYREYKHLIECCTNLRDKYLFHYKIFKEHGLG
                     PEVAHKLMVWDKNVMLFPRKIDDKFAMLHRIHPGIQLVLFDHWEDLTIEFWEDYFINL
                     ESHIVMDPMLRHESSHIGGGAPPIETEDGWLLIYHASESTPEGLTYHACAALLDLGNP
                     LIEISRLHEPLISPTEDWEKSGVVNNIIFPTGTVVQNDDLYIYYGAADERVGVARVSK
                     SELMSELKKQVKE"
     gene            14101..14748
                     /locus_tag="VGA21_09560"
     CDS             14101..14748
                     /locus_tag="VGA21_09560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650795.1"
                     /translation="MESKTLLKELLHLSLGAVVISADKVRKQIRKLVKKHKLTGDEGE
                     KALEQLGSKSKEIKEDFNKLITRISGKTKLAREEVEDFFEEMIEKPKEMKDKVDFMAR
                     KISINTSLKFEEARDELRDFIRDTSDIISRARSDAEKWINKGVAKSRETRKKGKEWLG
                     ELKEKSNHLEEKINPELKKVIDRTLEKLHLARTNDLEDLEKRLNSLEKKELTKMD"
     gene            complement(14769..15632)
                     /locus_tag="VGA21_09565"
     CDS             complement(14769..15632)
                     /locus_tag="VGA21_09565"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX9650796.1"
                     /translation="MKISQLLIPIDFSECSRNALVYALKLNKYFKAKITMVNCYTPSM
                     PVAGLKLQIDTDLEKGYKAETEKKFQKLEKEIPELQIIPYEYIIKMKLAPQGIIETAS
                     KSKTNLIVMGTRGTNTKLQTVFGSNAYSVIKESKIPVLAIPKDAKFKTLDRILVAADY
                     KDIRNTNPLELVREIAGISDSRLEILHINGKIKKMKESELSELLELRKLFSGMDFSFH
                     QLHTDKIDEGLETFVNQYRINMVVLLHRKHNLPESIFKKRITRKIALHFQVPILALPE
                     LPVALPKKLIM"
     gene            complement(15634..16470)
                     /locus_tag="VGA21_09570"
     CDS             complement(15634..16470)
                     /locus_tag="VGA21_09570"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX9650797.1"
                     /translation="MEKYKILVPVDFSPYSVNALKNAVSLAKKTDSFISIVHAVMVPV
                     VYGEDDMESSGWQAEEMEKGIKDNLESMTNLVPDLKDIDHEFKLEYALSPDAIITFTL
                     QNEVDLIVMGTRGKNRKGRALFGSNTFAVINSVKCPVLAIPEDGNINDVKKIILAVDY
                     DEIPPYASFEPLIKLARLFGAQVNIIHIGKEMKLTSEELLEARNLEQYFKTVKHQYKY
                     LFGGDEEKTISTYLKNENARVLAIISKEHTMVEKLQHRSLTKKIVSHLKIPLLVLHEN
                     SG"
     gene            16779..17810
                     /locus_tag="VGA21_09575"
     CDS             16779..17810
                     /locus_tag="VGA21_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009580434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HEX9650798.1"
                     /translation="MKALVLDKGEFDLSKGDEPLHIVEIPVREPLAEEVLIKITCCGI
                     CHTEIDEIEGRATPSFFPIVPGHQVIGVVEKTGNKVKQLKIGNRVGVAWIFSSCGNCE
                     FCLNGNENLCADFKGTGKDANGGYAEYITIGESFAYPIPEIFSDTEAAPLLCAGAIGY
                     RSLRLTNLADGQVLGLSGFGASGHLVLKMAKFLFPNSPVLVFARSPDEQTFARSLGAF
                     WAGDIKDGPPQKAHAIIDTTPVWNTVVGSMNHLLPGGRLAINAIRKESSDLNSLLQLD
                     YQKHLWMEKEIKSVANITRKDVVDFLEIAAGIPLKPEIQVYPFEQANQALIDIKQKHA
                     QGAKVLVMS"
     gene            complement(17839..18285)
                     /locus_tag="VGA21_09580"
     CDS             complement(17839..18285)
                     /locus_tag="VGA21_09580"
                     /inference="COORDINATES: protein motif:HMM:NF024304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX9650799.1"
                     /translation="MNKLNQNQIDYLLRSQMIGRIGCAADGKVYVVPITYIYDGTYII
                     GHSMEGMKIRMMRTNPNVCFQVEDIVSPVNWRSALVWGEYEEIKEVKEVRQIQELFRD
                     RLAPYQISEAGKPPDSFEKAPRVVEKPLMPIFFKIKITEKTGRFEK"
     gene            18617..20803
                     /locus_tag="VGA21_09585"
     CDS             18617..20803
                     /locus_tag="VGA21_09585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014770959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX9650800.1"
                     /translation="MNLKQKFYQTLFLLILNCTFVYSQNTYQATIQDKTTQEPLIGVS
                     VFIPELSKGNISDKNGSVIITNIPDGVYQVQFSYIGYQTLRFQVNFPLADSLNNNILY
                     LLPIAEEMEEVTISSTRSTRTIEDIPTRVEFIAGEELAEKGNMKPGDIRMMLNESTGI
                     QTQQTSATSYNSSIRIQGLDGKYTQLLRDGLPLYSGFSSGLSLMQIAPLDLKQVEVIK
                     GATSTLYGGGAIAGLVNLVSKTPRKESELSFMLNGTSALGLDASSFYSRKFKRFGATV
                     FTSYNLGSPYDPANIGLTAIPKFDRFTFNPKLFVYLNDKSDFNIGLNLITEDRLGGNI
                     DYVKGTQTANAYYEENETTRISTQISFEHRISTRSKIGLKNSYSNYDRKISIPNFTFA
                     GVQNSSFTELNYLHFSDRMEWVLGLNLWTDHFDQSTGSAGSPLDYQNATVGAFIQNIW
                     KASNKISLETGFRIDYHSEYEFFALPRLSFMYKPVQSLTLRVGGGLGYKTPTIFSEEA
                     ERIQFRNVLPLSVNDPDAEQSMGTNLDVNYRLNISKELLLTANILGFYTNIENPVVLI
                     QNNQSFFEFQQPDGNIDTKGAEVNLKLSYHDFKLFVGYTFADVNEHYNGITTKFPLVA
                     KHRLNNVLMFEKEENLWVGFEAYYFSPQKLSDGLTGQSYWILGLMSEKMFGEHFSIFL
                     NLENFLDTRQTRFDTIFTGTPDNPQFRDIYAPVDGFVINAGFKLKF"
BASE COUNT         6431 a         4041 c         4127 g         6205 t
ORIGIN      
        1 atcaataatg aagattttaa aaatctgaat tgctaatctt aattaaatcg ctattgctgc
       61 tatcataaat tatacactct atccgggaac catgtgaaaa tgttcgggaa tttgaaaaag
      121 aaattcaatt cgttgttaca tttgataaac gggttgcctc catagctcag ttggtagagc
      181 aacgctttcg taaagcgtgg gtcgcaggtt caagtcctgt tggaggctcg ttctaattgt
      241 taattgctaa gcgggagtag ctcagttggt agagcactag cttcccaagc tgggggtcgc
      301 gggttcgagt cccgtttccc gctcaaggta ttattcgctg aaatgttttc aaccggacca
      361 gaaatttatt ccgccagcaa agcaaacggg aagtccctaa actaaattcc tactctatga
      421 gctcatcttt attggaaaaa aatcccaggc tttatatttt cttaccccta ttatatatgg
      481 tgtgggaaga tgccgtgctt acgcccagtg aaatcaagac catgcaggat ttgatagaaa
      541 aacaggattg gctgacatca gaagaaatag atttcctttc cggttacctg aatccatcac
      601 agcctccttc accacaggaa ctgaaagagt ggcttgaaga atttagcaaa atcaaacttt
      661 ccgccgatac taaaactttg agtgaaattg gaattgaact tgccaaaaag aatggcgctg
      721 taaacggaaa cgagattgat gagaaaatca aaagtgacat catggaaatt gagaatgtgc
      781 tgggcatcat tagccgcgaa tcaattttca atttttatcc tgaaaaaaga aaaacattta
      841 cggcaagtca gaaaaccgaa caaacattta ctgttgagaa gttggctgaa atcctggatg
      901 ggagtcagcg gggtttaata aagaaaataa aactaattct ttccgatccg gaattcagat
      961 acttccatgg agacaatctt caggcaagaa gagagaaagt tctggaatgg tccagactgt
     1021 tggcaaagca gggattcggc gcacttgcct acccggggga atatggtggt aagaatgaca
     1081 tggaagctta ctttacagtg atggagactt taagctacca tgaccttagc ctggttatca
     1141 agttcggtgt tcagtttggt ctttggggta tgagtgttta cctgctgggc accgaaaagc
     1201 atcataaaaa atatcttaag aagattggta ctctcgaact gcccggatgt ttcgccatga
     1261 cggaaaccaa tcacggctcc aatgtaaaag gcattgaaac aacagcaaca taccatcata
     1321 caaccaaaac cattaccata aatactcctc accattacgc tcggaaaaat tatattggca
     1381 acgcggcatg tcacggacag gctgccactg tctttgcaaa attaattatt gagggcaagg
     1441 attatggagt aaatgcattt gttgttcctt tacgtgatga agcaggaaat actctttcag
     1501 gaattacaat agaagattgt ggcagaaaga tgggtctcaa tggtgtagat aacggtatca
     1561 ttcattttga taacgtacaa atacccctcg atgagatgct gaatcgtttc gccactatta
     1621 atgagcatgg agtttttgaa agtctcatct ccagtgataa cagaagattt tttactatgc
     1681 tgggaacatt ggttggcgga agaattggta ttccaagagc cgggttatca gcagccaaat
     1741 ccggtctcac catagccatt cgctatgctg atcagcgatt gcagtttgga ccggaaggag
     1801 ccaccgaagt acccatattg aattatcgca ctcatcaaag aaggttgatg ccacttattg
     1861 ccaatgccta tgcattacac tttgcgttgc aatatctaac gaaacgattt ctaaatcgca
     1921 ctgaagaaga aatgcaggaa gttgaagcat tggctgcggg cttaaaagca tttgccacct
     1981 ggaacaccac ggcaaccttg caggaatgcc gggaatgctg tggtggaaag gggtatatga
     2041 cagagaaccg gattgatgat ctaaaaaatg atacagacat tttcaccacg tttgaaggag
     2101 acaataccgt actcatgcaa ctggtggcca aaagcagact tacggaattt aagcaggaat
     2161 ttgggagtat gaatttgttc ggtattctga attacgtgat cgatcaggcg gccacttcct
     2221 tcatggaact gaatcccatt attgtgcgga aaacggatgc gggtcattta cttgatcccg
     2281 aatttcattt agatgctttt atttaccggg aaagagatat tctgacttcc gcggcccgca
     2341 ggcttaggaa gcatctggat aacggaatgg attcctttga cgcatttaac caaacgcaat
     2401 accatatggt gaatgtgggc ttcgcttatg tggagcgaat tatactggag caatttcaag
     2461 ttgctgtcaa aaatacaaaa gataaaggat gcaaaaatat ccttcaaaaa ttgtgtaacc
     2521 tgtttgcctt aagtcaactg gagaaaaata aaggctggta cctggagcat ggatatatgg
     2581 aaggagtgaa aacaaaagct atacgcaaac tgatcaatca gctggtatgg gagatccggc
     2641 aagaagcagt tcccctggta aatgcatttg aaatcccgga tagctgcctc tccgcaccaa
     2701 tcgctatcag gtagaagttc actttattcg ctttaattaa taaagatttc ctattaaact
     2761 gataaaaatc agtaatgaat ttttctaatc tttctaacta taggaaaaca attgaactat
     2821 gagaaaccaa aagcatgaat ttgatcattc ggtatttatt gttccacccg gcaagaaaac
     2881 caaattaagc aattttgatc ctgattatgt cggtcaaata aagggaaaaa agcacagtaa
     2941 agaaaccctc cgggaagata ttgatgatct ggcttcagcc caggaactcc tttgggcaag
     3001 tgccgaaaag agcatgttaa ttatttttca ggccctggat gctgcaggta aggatggcac
     3061 aattaagcat gtaatgacgg gtataaatcc ccagggatgc aatgtttaca gcttcaaagc
     3121 gccaacggag gaagaaagat tgcatcactt tttgtggagg cctaaccgtt ttttacctcc
     3181 acggggaaga atagcaattt ttaaccgatc atactatgag gaagtgctga ttgtgcgggt
     3241 gcacccggaa attctcaaat ccgcatggct ccccagggaa atgcgcaaaa tgtctccgga
     3301 aaaaatctgg aagacacgat ttaacgaaat aagggatact gaaaagcgat ggactcaaaa
     3361 cggaattcaa attgtcaagt tctttctgaa tgtcgggaaa agtgaacaga agaaaagatt
     3421 catggaaaga cttaacaatt cggataaaaa ctggaaattt tcagtcaatg attttaaaga
     3481 aaggcaatac tgggacggct acatgaaggc ttatgaagag atgttaaacg aaaccagcac
     3541 ttcatattcc ccgtggtatg tagttccggc aaataataaa tggtataccc gggcggttgt
     3601 tgctgatgtt atcaccaagc ggatttctga aatggatctg gcttttcctg cagttagtga
     3661 aagtactatg gcggagttaa gagaggttaa gaaaaaatta atggaagaat agttctaccc
     3721 gaaaattatt atgcagcaag tcaatcaaaa ggtgaaatac taatttattt tttttgcaat
     3781 tttgatattt cgttttccca attctttcaa aaacttatca tatagttctc cgctaataac
     3841 ttcttccgga gcaacaatac ctctttgagt aattgcatta gcagcgagca tcatgccggc
     3901 gattgatgca gagaaacccg taacccttgc catagaagtg atattgttga ctttatcttc
     3961 ttcatcccac ataaaaaact ccaccgtaca ttccttatta tccttaaatc cttttatcgt
     4021 attatacatt acgcaggcat cggcatcacc tttaaccggc cggagttttg gctccagctg
     4081 tgccagcata aactcacgag gtgaaatttt actaccttga aattcaacct gagtagtttc
     4141 taaaagtcca cactctttta atgtctgaat tccctgccaa tgacctggcc agcgaatagt
     4201 tttctcggta aaataatcga gttcaggatg tgtataaata aagcttggca tgccgggggt
     4261 aatgaagcat tcaagcttat catttttccc gaattcagtg aactcaaaaa tttcgtgatc
     4321 ctccaatgcc tgaacctgaa ccactttacc attttttcta atgctcaaac taaccatgta
     4381 ttccctgaga acatgttcaa acgaccaggt aataagatac ttgagaggat gcctgagtga
     4441 agtttccctg gaaggaatac ctcccactct cgctatcacc gaaaccgctt tatccaggta
     4501 atcaatggca gactgagtag taatattact tagacccgga gcaaagccca ttccatcaag
     4561 aattgtaacg tttgatttca tcgcacgatc gtgcaaactc tccccgtatt tctccagaga
     4621 catatcaccg ggaacttcca gtccttccgt ctcatgaggt tccggccgtc ggtgatattc
     4681 ttccaggata tcaacagcgt gggtcccggc attaatggca cattcaatgg ctttataact
     4741 gagcctgcgg ttcggcaagg taattattgc agcatcataa tcccggagca acaatgtaac
     4801 cgcttcctga ttctctaaat caactttgtg catgttaagc ttattgctat ttaatttgga
     4861 tgccattttt tccagtttca aatcacccat tccggtcact cccactgtat ttacagcgtt
     4921 gtttcgaaca aggtcatagg caaccgcaga acctattcta ccggtacccc caaggataat
     4981 tattctcata aaaatgtttg attgaacttt aagaaaagat aagctgtttt agaatgacaa
     5041 taataataag ggggaaaatt attttgtggg ttatgtattg aaaccgttca atccgggctc
     5101 acttattaat ttatgtccgg gtaattttat ggagtatgat attttgacag atcaaaccat
     5161 tctggcaaat cttttgcttt tataattgca taggttaaaa aacagctctt aagctgctat
     5221 tagaggttat ttataagaca agtgaagaag gcaattatct taatagcaac gttcttgttt
     5281 ttgcagttta atgttgcgaa cgcgcaatgg tttggtagaa ataagcctaa ctatgaacag
     5341 ttcaatttcc aggttaaaga aagcccaaat ttctctgtgt attattacgt aaagaatgat
     5401 tcacttgtgg aatcatttat taagagcagc gaaaaatggt ataaacatca tcagaaattc
     5461 tttcaggata ctttacagga gcgaaatccg atcatcctgt atggtgatca cactgatttt
     5521 cagcagacca ggattattag cgatttaatc ggtgtgggaa ccggcggtgt aacagagggt
     5581 ttaaaaaacc gggtagtaat gcccgtcatg gaattgaatt ctcaatttga tcacgtttta
     5641 ggccatgagt tggtccacgt atttcagtat aatttgctaa aaaccgggga ttccatttca
     5701 ctaaatcata taaggaattt accgttatgg atggtggaag gcatggccga atatttatcc
     5761 attggcagtt ttgatccaaa tacagccatg tggatgcgcg atgcggttct caacgatgat
     5821 atcccaacac tgaaagatct tactcaatca aataagtatt ttccgtaccg gtatgggcac
     5881 gccttttggt catttgttgg cggggtattt ggcgattcag ttgtggtccc tttgttcagg
     5941 gaaacagcac tgcatggtta tcagaatgcc atcaaacgta tttccggttt aaatgaaaaa
     6001 gaattttcgg aagcctggag tacacatctc aagaattatt ataaacagtt tgctcccgct
     6061 catcccgaca gtacattgga aaaagataat gttgtgggta aattactgat cgacccggaa
     6121 aaagccggta aattaaatat tgccccggta ataagtccta atggtcaata tgtggcattt
     6181 ctttccaata tgaatttgtt cacggttgac gtgcatctgg cggaaattaa aaccggaaaa
     6241 atcgtaaaaa cactatcctc agttgtaaaa aaccctcaag ttgatgattt cagctatatt
     6301 gaatcagcag gtacatggtc tccgttaagc gatcgctttg cattcgcagt tctctccagg
     6361 ggcaagataa agttgtcaat tgtagatatg ggccgccata agtcgcttac gatggaaata
     6421 cccggcgttc attattttag caacccaacc tggtctcctg atggcgaaag tattgtagtg
     6481 tccggacttg taaatgggca gagcgatctt ttcattttta acattaaaac caaagatgta
     6541 agacaactaa ccgaggattt ttactccgac attcagcctc aatggtccaa tgatggtcag
     6601 cgattaattt ttacttcaga tcgtccggtg gttggaagag agtatgatat gcatgcattg
     6661 cagatttgca gtttagatct tattaataaa caaataaccg ttcttgatat tttcccggga
     6721 tctgaaaacc tgaatcctgt gttcagtcca ggtgatactt ccttctattt tctttctaac
     6781 caggatggct tcagaaatct ataccaatat gacattgata gtcgaacaac ttataaatta
     6841 accgactatt acacgggcat tagtagtctt actccctatt ccccttcttt gagcgtttca
     6901 actaaaacgg gcaatttaag ttatgtttat tatggtaatc agacctatca aatatatacg
     6961 gcttcgcctg aagatttcaa taaagttcct ttagtggatg gaatggccaa taaaaatgcc
     7021 ggagttttac ctccatataa caagcaaaat attccgcaaa aaataaatct gaaagaggat
     7081 ttaagcccac ctgattccac catcagagac acaaagtacc ggcccaggtt tggcctgacc
     7141 tacattggaa acagtggcgg aataggtatt tctacaggaa cttttggatc gacggctcta
     7201 tccggtggaa taaatatggc ttttagtgat atgcttggtt accacaattt atatgctgca
     7261 atggcaatca acggtgagat ttacgatgcc ggcggacagg tagcatatat taaccaaaaa
     7321 cacaaaataa attggggtgt taccttgtca cacattcctt accgcacttc ttcgctgggc
     7381 tataaactgg attctctttc tgtggataac cagaattatt tgatggaaaa ttattttgtt
     7441 gatgtgtacc gaatgtttca ggaaagcgcg ggaatgtttt tttatttccc aacctcatcg
     7501 accaggcgat tcgaattagg ttcaagttat tcctattatt cattcagggg tgagagatac
     7561 aacaattatt attatcaagc catttatgtt aatcagcaaa aagaaaagat agaagctcct
     7621 ggcggatatt ggcttggaaa cacgtacgcg gcttatgtct ttgataattc tcactttgga
     7681 atcgcttcac cccaattggg cagaagatac cggatagaag caagtcaaac ctatggagaa
     7741 ttgcatttca gtacattgac attggatttc cgtaaatatt ttttcctcaa tcctacttct
     7801 gtagccttca gaattctaca ttcgggcata tacggaggag actcagagaa agtttatccc
     7861 atgagttttg cttatcctgg attaacaaga ggcaatagtc tggataacat tgaagtgtat
     7921 ggttacaatg aaaacaaacc atattcaata aatcaaatat ttggcagtag aatcctggta
     7981 gccaatgcag aatggagagt ttcatttacc ggacctgaac gcctggccct tataaaatcg
     8041 caatatttct tttctgagct cgcacttttt gttgatgccg gcatggcatg ggattctgct
     8101 cataacccca gctttgaact aaagcccaat tctacccagg accgggttcc tttcatcagc
     8161 gccggcctgt cttatcgcat taatttcttt ggccagttaa ttctggaacc ttattacgcc
     8221 tttccgtgga gaggggataa attcttgaaa ggtaactttg gaattaatat cgtgccgggt
     8281 tggtaattat taaattgatg attcctatat tatcagagtt tcaactctgc tttatttttt
     8341 agaacccaat aaagtccttt tacatttgat ttccatttag gtagaattac cacaataata
     8401 atttcttcca aaagatcagc tgtagttaca atagccgttc cataaaaaag ccaataaaca
     8461 ggttctccat agaagaaaag tgaaatgaag aaaaatccct ggaagaatgc ggctattttg
     8521 gctatgtagg tatggaaact ggtaggcttg ccatagcgtt ttaaagcaag gatggtttga
     8581 ataataaaga gtgttaacat ggcggcgatt atccaaatct cttccaaaag gaagtctgga
     8641 aaatgaacca caacgccgat gagcccggcc aggatcgtta aatcatctcc gatagaatca
     8701 agttttgccc caaggatgct acttgcattg tgcttgcgcg ccaggtatcc atctactata
     8761 tctgtgaaaa aactaaatgc aatcagccac tttacgatat ttaagtgacc tgtaaaaata
     8821 aaaagaagca ggtaaggaaa agaaaatatc cggtagaagg aaataagatt taccatcctc
     8881 acttcagctt tagacctgaa aagaaggtgc cagactttag gaaaaatttg cggaattttc
     8941 atccccatat attgaaacca ttaattatgg caaattaaca tgcctcaatg atatttatca
     9001 tcacaaaaaa tgacattgaa ggaattccaa ttatctccat ttgaaagtta attacgggat
     9061 tttcaacttt aaacaggctg gtgccttttt cccaaaatct tttctatatc ctctccaaat
     9121 acgatttctt tctttagaag taatttagct aataattcca gcttttcttt gttctccagg
     9181 aggagttttt cagtacgatt gcaggcattg ttaattaatt gtcttactcc atcatcaatt
     9241 aactttgcag ttgcttcacc ataatgtttt accagagatt tttcataatt gcctgattaa
     9301 aatcaataag tgggttgatg accctcatat atgaggcaac aaaatcaact tatatttatc
     9361 taaattcttc aactattaaa agctatgact gctataattg ataaattatt agatgagcgg
     9421 gggggcctat gcctttcctt aattaccact gcacacagga cctcccctga aaggagaatg
     9481 ggtcccatct taatgaagca gtcggtggaa aaagcagtta cattgattaa cacaaattat
     9541 gcctcacatc ctgacaagaa tattctcatc cggaacctcc accggatgaa agagactata
     9601 gactacaacc acctgaaaga aggattggga ctctttattt caaaagatat tcatgaactt
     9661 attcattttc cattccctgt tacagataag gttgaggttg acgacagatt cctgcttcga
     9721 gatctgcagt atctgaggca agtgaaaaaa gagtatttag tgctaagcgt tggaaagaaa
     9781 aaacttagag catttagagg aaatggagag tatctggctg aaatcataaa taatgatttt
     9841 cctgttattt ttcatgatga ttatgattat gcaagatcgg ttcgtggaac ctcttttggg
     9901 ctaaatgcag gaaaagattt tgaaaaagat aaatctatag ttaagcaaat caggatgaga
     9961 gatttctaca agtccgcgga tcagctggtg cataataatc tgaaaggaga tcaagaactg
    10021 gtggtttatg gcgatcagaa agaagtttcc gcgtacctgg aaattactca ggacaaagat
    10081 aaagtagttg acaaggtctt cagtcgctat ggagcaaagg gtgacaccca gcttaaatca
    10141 atggcaaaaa taaagatcaa aaattcgcag aaggagagtc ataagaaact tattgaagcc
    10201 attagagaat ttccggtaca aaaggtggcc attgggcttg gcaacatttg gaaagctgcc
    10261 tatctcggca aaggtcatca actggtggtt gaaaaagatt atacaataca tggatattta
    10321 tcaagtgatg gcagtaaact caaactcaga aggccccgat caaaagattc ctatgtcttt
    10381 ataaaagatg cggtggaaaa tgttttgaaa ctcgtgaaag atttgagtgg tgaaattatt
    10441 ttcctggata atgatgaact taaggacctg gatagaatcg cactaattaa gcgctattga
    10501 tttcagggtt tttctccata aaagctgcct tcataataat cttcggcaaa tgatgaatga
    10561 agcgttagac ttcatgctca accggttcct ctttttcaac atcaataatg ttcatactca
    10621 tttgtgaaat caggaaacaa actgttgatt ccgcaccctg gtttaaattg gctccggttt
    10681 cttccagccc atcgtaacaa ccaccggtat tcaaatcgaa catcacgcga ttaatcttat
    10741 tgtttcccaa aaaccagctg aaagcgattt tcttatattc ctcgtagttg ttcagctgca
    10801 gaattctgtg aaatgaatcc agtgcaagaa ttgtataagt tatatcaata ggctgttcac
    10861 caaaggattt tttggactca ccctttaact gccagcctct gtttgagaca accctgattt
    10921 gatcctcctt aaatgtttga gaaagcagaa aattgaaact gtttaacgca acatcaagat
    10981 atttggagta tccggtgatc tcgtaagcca taagaaccgc ttccggcatc acagcgtttg
    11041 cataggttag ataattttca aaccatctcc agcggaattg atttgaagag ttgtacatcc
    11101 caacaagttt atcagtcaat tttatgataa tctcagaaag atctttggag ggttcatgtt
    11161 tatagagata atatagacct ttaattataa atgccgctga acgtggtgag tcaatctcct
    11221 ccagccataa taagctttta gtgaatgata attttgcatc catcgatatt gatggcggca
    11281 aggagtcact tttggagatc aggaaaccga gtgcccaaat tgcgcggccg ttggagtcat
    11341 ttagatttac ataatgattt ctgtaatggt atttcccttg cgagtccagg taattaaaga
    11401 agcggccatc atcaagctga caaagctgga taaaatctaa aaattttcga attaagctta
    11461 agtcatttga attacctgtt ttcagataat gcatggttat tgcaatcaaa gccctggcgt
    11521 tatcatccaa cgtataccct gaagatagat ccggtacatt gattttggag aattgaacga
    11581 ttcctatgtc gtcgacaagg tttgtaaagt gcgacattct taattcgggc agattataat
    11641 ttattgtcca ggtagccgct gacttatcga gttcatgaaa aactttcatt aatttgatgg
    11701 ccacattttg ccaaacagac tcctgtgata tccggtaagc ttcaaagctt ttgcttttca
    11761 ggtcatcagg attctgaagg agtttaattg cagcttctgc cagctgtttg tgattatcga
    11821 aatcgaagaa ataccccacc tggttgctga gtagttccct ggcatgaggg aacggcgtgg
    11881 taatgacagg acaaccgcaa cttagtgcat aggcaaaagt accgcttact gcctgcttag
    11941 gatctttaga agtaaacaaa tacagatcag atagttgcag atactccagg agttcagggg
    12001 tgattaggta cctgtttata aatctgacat tcttctccaa tttcaagtcc ttcaccaatc
    12061 cctctaaaaa aatccggtat ttttctccat ctctttcaat aattttagga tgagttttac
    12121 caatcaccaa ataaaggata tccggaaata cttttataat atccggcaac gcatataagg
    12181 cagtctctat acttttattt tcactaatca atccaaaggt tgaaattatt tttttgtttc
    12241 caagttcata tctctttttc agtgcttcgg ggctactcca gctaacatga tgcgtgccgt
    12301 gggggatgat ccgaattttt tcctgcataa taccgtaatc ctggttcagg atcccggctg
    12361 aattatccgt catcacaata atcctttcca ctgaatctgc taacgcaact actaccttac
    12421 gcaactcagc gtttggtgcc ggcaaaacag aatgaaatat tatggttacg gggatatcaa
    12481 ctgcattaag gaattcaaat atatcgttac catactcacc tccgaataat ccgaattcat
    12541 gctggatgca aactccctta acatatctgt ctttattcaa ttgaaccgcg aaatccatgt
    12601 attgctgctt gtcagctgta ttcagcctgt acttaacttc atccggataa tcatattcgt
    12661 cagtttcatt ttcttcaagg gcacaaactt gccagttata gttttcgctg aataacgtat
    12721 caagagcctg aaccatgtcc tggcaaaaag ttgctatacc acattccctg tttgggtagg
    12781 tactcataaa aacaattttt tctttcgcga aggtcatttg atcattcttt tacttgtttc
    12841 tttaattcac tcatgagttc cgacttgctt accctggcaa cccctactct ttcatcagct
    12901 gcaccataat aaatgtagag gtcatcattt tgaactacgg taccggtggg aaaaattatg
    12961 ttatttacaa ctccggattt ttcccagtct tctgtgggag aaatcagagg ttcatgcaac
    13021 ctggagattt cgattagcgg atttcccaaa tcaagtaaag ctgcacatgc atggtaagtc
    13081 aatccctccg gtgtgctttc agatgcatga taaattaaaa gccatccatc ctcagtctca
    13141 atgggcggcg ctcctccacc aatatggctt gattcatgac gcaacatggg atccattaca
    13201 atatggcttt caagattaat gaaataatct tcccaaaatt ctatggttaa gtcttcccaa
    13261 tgatcaaata atactaattg aataccaggg tggatgcgat gaagcattgc aaatttgtca
    13321 tctatttttc ttggaaaaag catcacattt ttatcccata ccattaattt atgggcaact
    13381 tccggtccca gtccatgctc tttaaaaatt ttgtaatgaa aaagatattt atccctcagg
    13441 tttgtgcaac actctatcag atgtttgtat tcccggtagg taaatcttgg agttataatt
    13501 cctttctttt caaaatgatg taaatctttg gagattgcca atgcacccag cgcattggta
    13561 ttatcataag cgcaataggt cagataatag gtgtcctcaa ctttggtaat tcttggatcc
    13621 tcgattccct ggctttcgta atcatgttcg gggaatatca gcggcctttc atttctgcat
    13681 tttacaacca taggggactc caactcgcaa taacctatgg aggaataatt gccggttctc
    13741 actgcacggt aatagataat aattttccca ttatcctgga ttaccgcggg attaaataca
    13801 ccatcgttct cgaaaccatt gccggttttt tccagaagaa ttttcttttc tttaatttca
    13861 atcatcagtt ttgcctaaat aaggacattc acagcaagtt tctgatgatc ttaatcaaaa
    13921 tgcacggtga ttaatgtcac aaaaccggga accggaaaca gcctttgaga aatgttataa
    13981 aatgatataa atcattcagc aggacgaatt cagatcattg aattactcgg ctatatagat
    14041 caacttaagc cctgtaatga agtacaaagg tacacaagat aaattcaaaa gaagaatact
    14101 atggaaagca agacgttatt aaaggaatta ctgcacctga gcttaggagc tgtagtaata
    14161 tccgccgaca aagtcaggaa acagatcagg aagttagtta agaagcacaa attgaccgga
    14221 gatgaagggg agaaagccct tgaacaactg ggaagtaaat ctaaagagat taaagaggat
    14281 tttaacaagt taatcacccg gatttccggt aaaacaaaat tagctcgcga ggaagttgaa
    14341 gatttttttg aggaaatgat tgagaagccg aaagaaatga aggataaagt tgattttatg
    14401 gcgcggaaaa tctctattaa tacttctctg aaatttgaag aagccagaga tgagctaagg
    14461 gatttcatac gggacacctc tgatatcata agcagagcca gatctgacgc ggagaaatgg
    14521 attaataagg gagttgcaaa atccagggaa accaggaaga agggtaaaga atggctcggg
    14581 gaactgaaag aaaaatcaaa tcacctggaa gaaaaaatta accctgaatt aaagaaggtg
    14641 atagatcgaa ccctggaaaa acttcatctc gcgagaacaa acgaccttga agaccttgag
    14701 aagcggttga attctctgga aaaaaaggaa ttgactaaaa tggattaaga gactctaaga
    14761 atctacgcct acattattag cttctttggt aaagctacgg gcaattccgg caatgccaga
    14821 atgggaacct ggaaatgaag cgcaattttt cttgttatcc ttttcttaaa tattgattca
    14881 ggaagattat gctttctgtg aagcaatacc accatgttta ttctatattg attaacaaac
    14941 gtttctaatc cttcatcaat tttatcagtg tgcagttgat gaaaagaaaa atccatcccg
    15001 gaaaataatt tccttaattc cagaagttcg gataattcag attccttcat cttcttaatt
    15061 ttgccattga tgtgtaggat ttccaatctt gaatctgata ttcccgctat ctctcttacc
    15121 agttccaaag ggtttgtatt cctgatgtcc ttataatccg cggcaaccag gattctatcc
    15181 agagtcttga atttagcatc cttcggaatt gccaaaacag gaattttgga ttctttgatt
    15241 acagaatatg cattacttcc gaaaaccgtt tgcagtttag tatttgtacc ccgggttccc
    15301 atcacgatta ggttcgtttt gcttttggat gcggtttcta ttattccctg cggagccagt
    15361 ttcatcttaa taatgtattc atacggtata atttgtaact caggaatttc tttttccagt
    15421 ttttgaaatt ttttttcagt ttcagcttta taccctttct ccaaatcagt atcaatctgc
    15481 aacttcaacc cggccaccgg cattgatggt gtgtaacagt tgaccatagt tatttttgcc
    15541 ttgaaatact tgttcaactt taatgcatag accaaagcat tcctggaaca ctctgaaaag
    15601 tctattggta ttaataattg cgatattttc atcttacccg gaattttcat gtaaaaccaa
    15661 caaaggaatt ttgaggtgac ttactatttt tttagttaaa gatcgatgct gaagcttttc
    15721 caccatagta tgctccttgg aaattatcgc cagaacccgg gcattttcat tcttcaaata
    15781 ggtcgagata gtcttttctt cgtctcctcc gaatagatac ttgtattgat gcttgactgt
    15841 cttaaaatac tgctccaggt tcctggcttc aagcaattct tcacttgtta atttcatttc
    15901 cttgccgata tgaataatat tcacctgtgc gccaaaaagt cttgccagtt ttatcagtgg
    15961 ctcaaaagag gcatagggag gaatttcatc ataatcaacc gctaaaataa ttttctttac
    16021 atcattaatg ttgccatcct ctggaatcgc cagcacagga cactttacac tgttaattac
    16081 ggcgaatgta ttactgccaa atagggctct ccctttcctg tttttgccac gagtgcccat
    16141 aacaatcaaa tccacctcat tttgaagcgt gaatgtaatg atggcatctg gcgaaagtgc
    16201 atattccaac ttgaactcgt gatcaatatc tttcaggtca ggaacaagat tagtcatgct
    16261 ctccagattg tcttttattc ctttttccat ttcctctgcc tgccaacctg aagattccat
    16321 atcatcttct ccatatacga caggtaccat tacggcatgt acaattgata tgaatgaatc
    16381 tgtcttcttt gccagggaaa cagcattttt cagagcattt actgagtacg gcgagaaatc
    16441 aaccggaacg agtatcttat atttttccat ttctgagaac atttgagttg tacaagtttt
    16501 taagaaatgg ccggacttaa tgtttaccta atacctaaac tttgaggtct cagccattct
    16561 caaatggaga acgttgttta ctcctccatt tcaacgacaa tgtattccga ctacaatgcc
    16621 taaacaatga tcttaatcaa cagaggattt gactattatc attaagggga aagctgaaat
    16681 ttgggtggaa ttacaggaaa atagaaaatc tggtgagatt atttcaatat catggattgt
    16741 gatttgtttt tcgggcgata acttgtttaa ttattggaat gaaagctttg gtacttgata
    16801 aaggagaatt tgatcttagt aaaggtgatg agccattaca tatagtagag attcctgtaa
    16861 gggagcctct ggctgaagag gtactgatca aaattacatg ttgcggtatt tgccacacag
    16921 aaatagatga aatagaaggc agagcgacac cttcattttt cccaatagtt cccggacacc
    16981 aggtaatagg agttgttgaa aagaccggga ataaagtaaa gcaattaaaa attggaaacc
    17041 gggttggagt tgcctggata ttttccagct gcggtaattg tgaattttgt ttaaatggta
    17101 atgaaaattt gtgtgctgat tttaaaggaa caggcaaaga tgcaaatgga ggatacgcgg
    17161 agtatattac aataggtgaa agtttcgctt atccaattcc ggagattttt tccgatactg
    17221 aagcggctcc tttgctttgt gccggagcta ttggctatcg ttcattacga ttaacaaacc
    17281 tggccgatgg gcaagtgctt ggactatccg gctttggggc atcaggccac ctggtattga
    17341 aaatggcgaa atttctattc cccaattctc ctgtactcgt atttgcaaga agtcccgatg
    17401 agcaaacttt tgccagatct ttgggcgctt tttgggccgg cgacatcaag gacggcccac
    17461 ctcaaaaagc acatgcaatt attgacacca ctccggtttg gaatacagtg gtgggttcca
    17521 tgaatcatct gttaccggga ggcagacttg ctataaatgc cattcggaaa gaatccagtg
    17581 atttgaattc gttacttcaa cttgattatc agaaacatct gtggatggaa aaagagataa
    17641 aaagtgtagc aaacattacc agaaaagatg ttgtggattt tcttgaaatt gcagccggta
    17701 ttccactaaa gccagaaatt caggtctacc catttgagca agcaaatcag gcattgattg
    17761 atatcaaaca aaaacatgcc cagggagcaa aggttttagt aatgagttaa aacaattcca
    17821 tactgacaat gacagaaatt acttttcaaa tcgccctgtt ttctcggtaa tttttatttt
    17881 gaaaaaaata ggcatcaggg gtttttctac cacccggggt gctttttcaa aactatcggg
    17941 aggcttacct gcttcactta tctgataagg ggcaagtctg tctcgaaaca attcctgaat
    18001 ttgccgaact tccttcactt cttttatttc ctcatattct ccccatacca gtgccgacct
    18061 ccagtttacg ggtgagacaa tgtcttccac ctgaaaacac acgttgggat tcgtgcgcat
    18121 cattcttatt ttcattcctt ccatgctgtg tcctattata tatgttccat cataaatata
    18181 agtaatagga accacataaa ctttgccatc tgccgcacaa ccaattcggc cgatcatctg
    18241 acttctcagc aaataatcaa tttgattttg attaagctta ttcattgtga taaattctga
    18301 accagacgtt tcctacactg atttgttatc aatttacagc cataacctga tttcagctat
    18361 gattttcttc atttcctggg aaaaacagaa tcaggcttca acaactttta tatgatgtca
    18421 atcatgtatt tgagtatcaa aaatcctgca attggaatta tagtttctcc tcatgaacag
    18481 taaagtaaga gatttgttta aaagtgatgt ataccattac ggattgtccg gatggtgatt
    18541 taaaacgcta actttggaaa ttgcaaacta gaataaatca aagagaagaa atgattaaac
    18601 cattcaccgg ttggaaatga atttaaaaca gaaattttat caaactctat ttttactgat
    18661 cctgaattgc actttcgtct attcacaaaa tacctatcag gctacaattc aggataaaac
    18721 aacacaagaa cctttaattg gtgtttcggt atttattcct gaactgtcga aagggaatat
    18781 ttcagataaa aacgggagtg taattattac caatattcct gacggggttt accaggtaca
    18841 gtttagttat atcggttacc agacactcag gtttcaggta aacttcccat tggccgactc
    18901 tctgaataac aacatattgt atttgttacc cattgctgag gaaatggaag aagttacaat
    18961 ttcttcgaca cgaagcacca gaactattga agatatcccg acacgagtag aatttattgc
    19021 aggggaagaa ttagcagaaa aaggaaatat gaaacccggt gatattcgga tgatgttaaa
    19081 tgagagtacc ggaatacaga cccagcaaac atcggcaacc tcctataact ccagtattcg
    19141 catacaaggc cttgatggta aatatacgca attgctccgc gatggtctgc cactttattc
    19201 gggcttttcc agtgggttaa gcctgatgca aattgcaccg cttgatctga aacaagtgga
    19261 ggttattaag ggagcaacat caacattata cggaggtggt gcaatcgcgg gactcgtgaa
    19321 cctggtttcc aaaacaccac gcaaagaatc tgaactaagt tttatgttga acgggacttc
    19381 cgcattgggt ttggatgcca gcagttttta ctccaggaaa ttcaagaggt tcggtgctac
    19441 tgttttcaca tcatataatc ttggctcacc gtatgatccg gcaaatattg gcctcactgc
    19501 aataccgaaa tttgatcgct ttacattcaa ccctaagtta tttgtttatc ttaatgataa
    19561 aagtgatttc aatatcgggt taaacctaat cacggaagat cgattaggag gcaatatcga
    19621 ctatgttaaa ggaacccaaa cagcaaatgc ttattatgaa gaaaatgaaa ctacacgtat
    19681 ttctacccag atttcgtttg agcacaggat aagtacccgg tctaaaatag gcctgaagaa
    19741 tagttacagc aattatgatc gaaagatttc cattcccaat ttcacgtttg caggagtaca
    19801 aaattcttcg tttaccgaat tgaattacct gcatttcagc gaccggatgg aatgggtatt
    19861 gggtttgaat ttgtggaccg atcattttga tcaatcaaca ggttcagcag gatcgcccct
    19921 ggactaccaa aatgctacag tcggcgcttt tattcaaaat atctggaagg caagtaacaa
    19981 aatttccctg gaaacaggtt ttagaattga ctaccacagt gaatatgaat ttttcgcatt
    20041 acccaggctt tccttcatgt ataaacccgt tcaatctctc acattgagag ttggtggtgg
    20101 attaggttat aaaacaccaa caattttttc cgaagaagcc gagcgcatcc agttccggaa
    20161 tgtgttgccc ttaagtgtta atgatcctga tgcggaacaa tcgatgggaa ccaaccttga
    20221 tgtaaactac aggttaaata tctccaaaga gttattgtta actgcaaaca tacttggctt
    20281 ctatactaat attgaaaatc cggtcgtcct tattcagaat aaccaaagtt ttttcgagtt
    20341 tcagcaacct gatggaaata ttgatacaaa aggagcggaa gtaaatctaa aactaagtta
    20401 ccatgatttc aaattattcg tggggtacac ctttgcagat gtaaacgaac attataatgg
    20461 cattactact aaatttccgc tggtggcaaa acatcgcctg aataatgtat taatgtttga
    20521 aaaggaagaa aatctttggg tcggttttga agcctattat tttagtccgc aaaaattaag
    20581 tgacggatta accggacaat cttactggat tttaggttta atgagtgaga aaatgttcgg
    20641 agaacacttt tccatttttc ttaatcttga aaactttctt gatacccggc aaacccggtt
    20701 tgatacaatt tttaccggca caccggataa cccacagttc agagatattt acgcacccgt
    20761 ggatgggttt gttatcaatg ctgggttcaa attaaaattc tgaa
//
LOCUS       DASWHX010000142         3725 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_3446936, whole genome shotgun sequence.
ACCESSION   DASWHX010000142 DASWHX010000000
VERSION     DASWHX010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 3725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3725
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3446936"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..693
                     /locus_tag="VGA21_09590"
     CDS             <1..693
                     /locus_tag="VGA21_09590"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HEX9650801.1"
                     /translation="IAFDNKMEEQAREWLQTWDFVLDRNSVEAGIYVEWETQIRREAN
                     MRFIPVEVRGSINLQLTRIITYLSSPNEMFGNDFETNRIDFLKKTFSTAINTLAEKFG
                     NNMEDWVYGQPGYKHALIYHPLSRAVNKEFQEKFNVGPAPRGGNGYTPGSTGNGLNQS
                     SGASFRVIIPVGDWDNSLGINSPGQSGDPESPFYRNLFEMWANDEYFPMYYSKEKIES
                     VMAEKYRLVPKN"
     gene            706..1503
                     /locus_tag="VGA21_09595"
     CDS             706..1503
                     /locus_tag="VGA21_09595"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020528942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX9650802.1"
                     /translation="MKKVFLPAILVSASWIAAAQTPEERLEKSPRHQEWVEVKQGDKT
                     VHSFLVYPEVSNQVPAIILIHENRGLNDWVRGMADQFAERGYIAIAPDLLSGMGPAGG
                     KTSDFETGDAAREALYQLDPDQITKDLNAVYDHVSKLPSSNGKVIVIGFCWGGSQSFR
                     FATNQPNLSAAMVCYGSPPDSGYENIKAPVYGFYGGSDNRINASIPDAEKKMKELSKE
                     YDYVIYEGAGHGFMRSGEADEGSAENKKAREEGWKRIEKILSEIKGK"
     gene            complement(1514..1948)
                     /locus_tag="VGA21_09600"
     CDS             complement(1514..1948)
                     /locus_tag="VGA21_09600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650803.1"
                     /translation="MKNVLMMMATFVLLAAGCVEDKLDLDDVPIGVDFTLPKGEKAIV
                     DAVNGKLSIEIENVYDSRCPANAICILEGNYGVDLMISDATQSTPVKLCNLFCGSDYK
                     LRDTASFVLNETEYQIVLKDLQPYPGTIENDEPKKVILEILK"
     gene            2106..2936
                     /gene="ygiD"
                     /locus_tag="VGA21_09605"
     CDS             2106..2936
                     /gene="ygiD"
                     /locus_tag="VGA21_09605"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014773664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HEX9650804.1"
                     /translation="MQRKLPEELLDLPETPVMPVLFIGHGNPMNAIEENFYTNEWKKI
                     ASGLTVPKAILCISAHWLTKGTWVTAMEKPRTIHDFYGFPKELFAVNYPATGAPQFAE
                     FTKSLVKKTEIGLDHDWGLDHGTWSVLNQMFPSADIPVYQLSIDYHKPANYHYELAGE
                     LKKLRAKGVLIIGSGNIVHNLRIMRFEDLSYDWTIEFDAMAKNFIWTRDDEALIDYEK
                     HGEIAKLSIPTPDHFFPLLYTLGVTERNEIINFFSEKTTMGSISMRSVLIKKKAELIF
                     "
     gene            complement(2994..>3725)
                     /locus_tag="VGA21_09610"
     CDS             complement(2994..>3725)
                     /locus_tag="VGA21_09610"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX9650805.1"
                     /translation="SVGAEWEYKFSNHNPFDPLKSGGLKAVTFITHKASFKWGIIFDQ
                     PTYSIGISYSTSSLSNRNSVIIPGLEFGIKIKINKKKKRKLVLESSSVSEVRSFFKEN
                     INEKDKVINQEDDAIVSLNKMQDMRFELRKDFNFVFNDAELNNEAKEYLDDIVNLLKE
                     NDLLHLKAIGHTDDIGSKQTNLSISRRRAESAINYLISQGIDSKRLRSIGKGDSDHLY
                     PNETEENRSRNRRVELILYFPGKED"
BASE COUNT         1151 a          707 c          764 g         1103 t
ORIGIN      
        1 atagcatttg ataataaaat ggaagagcag gcccgggaat ggctacaaac ctgggatttt
       61 gtattggatc gaaattccgt agaagccggt atttacgtgg aatgggaaac ccaaatcagg
      121 cgtgaagcaa atatgcggtt cattccggtt gaagtcaggg gttcaattaa tttgcagcta
      181 acccgtatta ttacttattt gtcttctcct aatgaaatgt ttggaaacga tttcgaaaca
      241 aatcgaattg attttctgaa aaagactttt tcaaccgcca tcaatactct cgcggaaaag
      301 tttggcaaca acatggagga ttgggtatac ggtcagccag gttacaagca tgcccttatt
      361 taccatccac taagtcgggc tgtaaataag gaatttcagg aaaagtttaa tgttggacct
      421 gcccccaggg gcggcaatgg ttatactccg ggatctacgg gcaatggtct aaatcaatca
      481 agcggtgcca gtttcagagt aataatacca gtcggggact gggataattc tttgggcata
      541 aattctcccg gacaatcagg tgaccctgaa agtcctttct atagaaattt gttcgaaatg
      601 tgggcaaatg atgagtattt tcccatgtat tattccaaag aaaaaataga atctgttatg
      661 gcagaaaagt atagattagt tcccaaaaat taaatgataa taataatgaa aaaagtattc
      721 ttaccagcaa ttcttgtgtc agcgagctgg atagccgcgg cgcaaacgcc tgaagaaaga
      781 ctggagaaat ctccgcgtca ccaggagtgg gtggaagtaa agcaaggcga taagaccgta
      841 cattcttttc tggtatatcc ggaagtgagt aaccaagtac cggcaataat tcttattcat
      901 gagaacagag gtttgaatga ctgggttcgt ggaatggcag accagtttgc agaacgcggt
      961 tatattgcca ttgcccctga tttactctcc ggaatgggcc ccgccggtgg caagaccagc
     1021 gactttgaaa ccggtgatgc agcccgtgaa gctttgtacc aattggatcc tgatcaaatc
     1081 accaaagacc tcaatgcagt ttatgatcat gtttctaaac tgccgtcatc caatggtaaa
     1141 gtaatagtaa taggcttttg ctggggtggc agccagagtt tcaggtttgc aaccaatcag
     1201 ccaaatctgt cagctgcaat ggtttgttat ggttctccgc ctgatagcgg ctacgaaaac
     1261 ataaaagctc ccgtctatgg attttatggc ggaagcgaca acaggataaa tgcttctatt
     1321 cccgatgcgg aaaagaaaat gaaagagtta tcaaaggaat atgattatgt gatttatgaa
     1381 ggcgccggac atgggtttat gcggtccgga gaagcggacg agggatccgc agaaaataag
     1441 aaggcgcggg aagaaggttg gaagcgaatt gaaaaaattc tatccgagat taaaggaaag
     1501 tagggctaat gccctacttt aatatttcca ggataacctt ttttggctca tcattctcta
     1561 tcgtgcccgg atagggctgc aaatctttta atacaatttg gtattcagtt tcattcaaaa
     1621 caaaactggc ggtatctcgc agtttgtaat cgctgccgca gaacaaatta cacaacttaa
     1681 ccggggtcga ctgagttgca tccgaaatca ttaaatcaac tccgtaattt ccttctaaaa
     1741 tgcaaatggc attggcagga cacctactgt catagacatt ctcaatttct atggagagtt
     1801 tgccgtttac cgcatccaca atagcttttt cacctttggg aagcgtaaag tcaactccga
     1861 taggtacgtc atcgagatca agtttgtctt ctacacaacc cgcagccagt aaaacaaagg
     1921 ttgccatcat catcaaaaca tttttcataa agttcttaat ttgttttttc atcattagac
     1981 cctccatccg tgttaaaggt tggaaattta acactcatcg ctaattcgta atacggattg
     2041 cttaaatatt tggcaaactt atttgataaa tttgttgtta ctaaattgta gtgcaagaat
     2101 ttagaatgca acgaaaacta ccggaagaat tattggattt accggaaacg ccggtcatgc
     2161 ccgtactttt tattggtcat ggaaatccaa tgaatgccat tgaagaaaat ttttatacca
     2221 atgaatggaa aaagattgct tccggtttaa cggttcccaa agctatactt tgtatttctg
     2281 cccactggct aactaagggt acgtgggtca ccgctatgga aaagccaaga actatccatg
     2341 atttctatgg atttccaaag gaattatttg cggtaaatta tcccgctacg ggtgctccgc
     2401 aattcgcgga attcacaaaa tctcttgtta aaaaaactga aataggtttg gatcatgatt
     2461 ggggtctgga tcacggcacc tggtccgttc tgaatcaaat gtttccttcc gccgatattc
     2521 ctgtttatca actaagcata gattatcaca aacccgcaaa ttatcattac gagctggctg
     2581 gcgaacttaa gaaactgcgc gcgaaaggcg tcctgataat aggaagcgga aacatcgttc
     2641 acaatctgag aattatgcgc tttgaagatt tgagttacga ttggacaatt gaatttgatg
     2701 caatggctaa gaatttcatt tggaccaggg atgatgaggc tttaattgac tacgaaaaac
     2761 atggagagat cgctaaatta tcaatcccaa cccctgacca tttttttccg ctattgtaca
     2821 ccctgggagt aacggaaaga aatgaaataa ttaatttctt ttcagaaaag accacgatgg
     2881 gttctatcag catgaggtcg gtgttgataa aaaagaaagc tgaattaatt ttttgaagaa
     2941 aactgcttta caaaagtttg attggcaaga taatcaggaa tggcaaatca taattagtct
     3001 tctttgccgg ggaaataaag aataagttct actctgcgat ttcgacttcg attttcttca
     3061 gtctcgtttg gataaagatg atcagagtct cctttaccaa ttgaacgcaa tcgtttacta
     3121 tctatcccct gactaattaa ataattaatg gctgattccg ctcttcttct tgaaatagaa
     3181 agattggttt gtttactgcc aatgtcatca gtatgcccga ttgctttaag atgaagcaaa
     3241 tcattttctt tcaataagtt aacaatatca tccagatatt cttttgcttc attattaagt
     3301 tctgcatcat taaaaacaaa attgaaatct ttccttaact caaatctcat atcctgcatt
     3361 ttatttaaag atacaattgc atcatcttct tgattaatta ctttatcttt ctcatttatg
     3421 ttttctttaa agaatgatcg aacttctgaa actgaagaag attcaagaac caattttcgc
     3481 tttttctttt tattaatttt tattttaatt ccaaattcca gaccgggaat aataacagaa
     3541 tttctgtttg ataaggagga tgttgagtaa cttatgccga tgctatacgt cggttgatca
     3601 aaaataatcc cccatttgaa agaagcctta tgagtgataa atgtaactgc tttcaagccg
     3661 cctgatttga gaggatcaaa cggattgtgg ttagagaact tatactccca ttcagctcca
     3721 actga
//
LOCUS       DASWHX010000143         2843 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_876298, whole genome shotgun sequence.
ACCESSION   DASWHX010000143 DASWHX010000000
VERSION     DASWHX010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 2843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2843
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_876298"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            26..1855
                     /locus_tag="VGA21_09615"
     CDS             26..1855
                     /locus_tag="VGA21_09615"
                     /inference="COORDINATES: protein motif:HMM:NF017390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HEX9650806.1"
                     /translation="MEFVNEIIQNRASSQIGWSLLHSLWQGLTIYLVLRLILTVFISG
                     NPVFRYKLYYAALVTQITWFSVTLFKYLNIETGSTLLVPAKDFSLLIPETTTKISVWY
                     SVVEFLNQQAPLIFVLWVGGITFLTMRFLGGIYQVHVLKTQKILPVDYELDLLFNRLL
                     ERLNFSRRVRIFLSEKVNLPVAAGFLKPVVLIPIGFVNGLTQQQVESVLLHELAHIKR
                     NDYLLNLLQSIVDIIFFFNPFTWLISRNIRMEREQACDDFALKYTHKIEYARALIATR
                     EFQANHSIAQGFHNGKNELFTRINRIMGKLKLNEKSHSFRNMVAYTIVLGMLALTLAF
                     SNKNENTIDQAPAGTEKLYPASKGDLTARMAIFESVDEEKLPDESNKHVNKNLETEEE
                     SGKSNLQLKRLNNILSVVEIDSPEIDNQKLKKLVEEMKAHQEDWARLLDEDQRKLTEA
                     MKELKTINLTIDPITLQELEKLRELQIQLGKQNIQNLQVSMMELREQMEQMNLELMNR
                     EEMKKMQEEMMRAQKEIKVMEEEIKVMEEKMAAAIKELREAAVNDGYLKSKSSEMSID
                     FEDDGIYINHQLVKKEHEEKYRQVIKKYFPREKGRFRIQDNQD"
     gene            2499..>2843
                     /locus_tag="VGA21_09620"
     CDS             2499..>2843
                     /locus_tag="VGA21_09620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650807.1"
                     /translation="MVSYEKMGISYGIIKTRFETGQHKRAKKNRMEIEGFYKEVLEQL
                     IKNEVEFLLVGGLAVGFHGYARFTGDMDLWLKPSNDNLERLGNALGDLNYSKKIVDDI
                     IESRPIDHPTPIR"
BASE COUNT         1023 a          446 c          602 g          772 t
ORIGIN      
        1 gaaaagggtc taaaataatc aggttatgga atttgtaaat gaaataatcc aaaatcgcgc
       61 ttcatcccaa ataggatgga gtttgctgca cagtttgtgg cagggactta ccatttacct
      121 ggttctgaga cttatcttaa cggtatttat atctggcaac cctgttttca gatacaagct
      181 ttattatgcc gcgctggtca cacaaattac ctggttttcc gtaacactat tcaaatacct
      241 aaatattgaa accggatcta ccctattggt tcctgctaaa gattttagcc ttcttattcc
      301 cgaaaccacc accaaaattt cagtttggta ctcagtggtg gaattcctta atcaacaggc
      361 gcccctgata tttgtgcttt gggtgggcgg cattacattt ctgacaatgc gatttcttgg
      421 cggaatatac caggttcatg ttttgaaaac acaaaaaata ttgccggttg attatgagtt
      481 agatctttta ttcaatcgat tgcttgaaag attgaatttt tcccgccggg tgagaatctt
      541 tctgagcgaa aaagtaaatc tgccggttgc agctggattt ttaaaaccag tcgtactaat
      601 accaattggc tttgtaaacg gtctaacaca gcaacaggta gaatctgttt tacttcatga
      661 attagctcac attaaacgaa atgattatct gttaaacctt ttgcaatcaa tagtggacat
      721 tattttcttc ttcaatccgt ttacctggtt aatttcacga aatatcagga tggaacgaga
      781 gcaagcttgt gacgattttg cgttgaagta cacacataaa attgagtatg cccgtgcact
      841 gattgcaaca cgggaattcc aggcaaatca ttcaattgct cagggttttc ataacgggaa
      901 aaatgaatta tttactcgaa taaacagaat tatgggaaag ttaaagctca acgaaaaaag
      961 ccattcattc aggaatatgg tggcatatac gattgtttta ggcatgttgg cactgacgct
     1021 ggcatttagc aacaagaatg agaataccat tgatcaggca ccggctggta ctgaaaaatt
     1081 gtatccggcg tctaaaggag atctgactgc aaggatggcg atttttgaaa gtgtggatga
     1141 agagaaatta ccagatgaaa gtaataagca tgtgaacaaa aatcttgaaa cagaagagga
     1201 atcgggcaaa agtaatttgc aactcaaaag actgaataac atattatccg tggttgaaat
     1261 tgattctcct gaaattgata accaaaaatt gaaaaagttg gttgaggaaa tgaaagcgca
     1321 ccaggaagat tgggcaagac ttcttgatga agatcaaagg aaactgaccg aagccatgaa
     1381 agaactgaag actattaatc tcaccattga ccctatcacc cttcaggaat tagagaaact
     1441 tcgtgaattg caaattcaat tgggtaagca aaatatccag aacctccagg tgagcatgat
     1501 ggagctgagg gaacaaatgg aacaaatgaa tttagaattg atgaacaggg aagaaatgaa
     1561 gaaaatgcaa gaagaaatga tgcgcgccca aaaagagata aaagtgatgg aggaagaaat
     1621 caaggtaatg gaagaaaaaa tggcagcagc aatcaaagaa ttaagagagg cggcagttaa
     1681 tgatggatat ctgaaatcaa aatcttctga aatgagcata gattttgaag atgatggaat
     1741 atatattaat catcagttgg taaagaagga gcacgaagag aagtatagac aggtcatcaa
     1801 aaaatatttt ccaagggaaa aaggacgatt tagaattcag gataaccagg actagaaagg
     1861 ttggcgtggg gaaggtagtg actaagtaac tgtgtaaagg cccgatttgg ccaaggcggc
     1921 agtagcgaaa tttttttgtg aatgtttctc aaacctcgat tggaaatatt tcaaaaaatg
     1981 tagtggctat gcgaaaaaga tgtttttttg taaaaaatcg gactatatta ctaatgatga
     2041 agacgatttt acagaaatag aagaatattt taagtcccac taaaggctaa ttattattgg
     2101 ttcaaataaa aacactggaa aaatttgaaa gggagttcaa cgcagtatat caaacatact
     2161 gggttgctac ttctgaaaac aggaattatt tccgaacaaa tgaattggaa tataccgaag
     2221 ataaccgtcc agacataaag aacacaaaat ggacacacat ctcaaatcct acgcatttgc
     2281 aaataacaga acaaatgaaa cggactaaag tttttgaata tcaaaaacaa cctatgagtt
     2341 caaaggtttt aaaaaagccc aagaataaag acatggggat gtcaatgtag tatcattaaa
     2401 cgctaaaatc gattaattat gtccagaaag gtagagtgga atccggatca taaaaacgca
     2461 gatgatcagc agaatacttt tattgataga tacttgtcat ggtctcctat gaaaaaatgg
     2521 gaatatctta tggaattatc aaaacaaggt ttgaaacagg tcaacacaaa agggccaaga
     2581 agaatagaat ggaaatagaa ggtttttata aggaggtatt ggaacaactc attaaaaatg
     2641 aagttgaatt tctccttgtt ggtgggcttg cagttggatt tcatggatat gccaggttta
     2701 ccggagatat ggatttatgg ttaaaaccgt caaatgataa tttggagcga ttgggtaatg
     2761 cgcttggtga tctcaactat tcaaaaaaaa tagttgatga tattattgaa tcaaggccta
     2821 tcgatcatcc aactccaatt cgt
//
LOCUS       DASWHX010000144        49918 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_2734295, whole genome shotgun sequence.
ACCESSION   DASWHX010000144 DASWHX010000000
VERSION     DASWHX010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 49918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49918
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2734295"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..508)
                     /locus_tag="VGA21_09625"
     CDS             complement(<1..508)
                     /locus_tag="VGA21_09625"
                     /inference="COORDINATES: protein motif:HMM:NF013660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase"
                     /protein_id="HEX9650808.1"
                     /translation="MSVEEQDKIILIDKPTGWTSFDVVKKIRNCLGIRKVGHAGTLDP
                     LATGLLILCTGKKTKEINEIQEMEKEYIGEFTLGQITDSFDLETPVKFVSNCKNVTPD
                     SVIETAKKFVGTVDQIPPAFSAIKVEGKRAYLKARKGEKQSIQPRKVTLYSFEILKIE
                     IPLIAFKVI"
     gene            complement(509..1297)
                     /locus_tag="VGA21_09630"
     CDS             complement(509..1297)
                     /locus_tag="VGA21_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019596932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEX9650809.1"
                     /translation="MTNFEAFILGVLQGLTEFLPISSSGHLEIGQALFGLHPGNELLF
                     TVILHGATVLSTIVVFRKEIKFILSGISLKGNNEQSSYLIKLLISAIPIAIVGLVFES
                     EIKALFFGNIGIVGIMLLITGLILVFAHFNRSREKPIGYFHSIIIGLVQVVAILPGIS
                     RSGATISTALLLGISREEAARFSFLMVLLPIIGANAKIIFSGGWLEEGYSIQPLLIGF
                     IAAFISGYIACKWMLSIVKSGKLLYFAIYCFILGMSALIFHYRF"
     gene            complement(1298..1507)
                     /locus_tag="VGA21_09635"
     CDS             complement(1298..1507)
                     /locus_tag="VGA21_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009195716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3098 domain-containing protein"
                     /protein_id="HEX9650810.1"
                     /translation="MSKKENLAFQRINYILLIVGILAIALGFTIMSLDKDPHGFGVLG
                     LTIGPLVVMSGFVLEFFAILYRPRK"
     gene            complement(1509..2402)
                     /locus_tag="VGA21_09640"
     CDS             complement(1509..2402)
                     /locus_tag="VGA21_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017730826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HEX9650811.1"
                     /translation="MEKPIKTKKKKKLGSYPYISVVFSITLALFVFGLFASLIIHSQK
                     LATLIRENVEIQVYLRKNLSENQQISIRQSLASQDFVATNSGEPRIRFISKEEAAKDF
                     VENTGEDFDFLGENPLRDAFIINLNEKYQSEFKMDSIRSEINNISGVFEVEYVDNLIS
                     SINQNITKIGLVLVGFTFILVVVVIILIGNTIKLALFSQRFLIRSMQLVGATKAFILK
                     PFLLRSAFHGFLGGVFASVAILVVLNYSYKTIDELVALRDQRLIMILLGSLILSGIVL
                     GFFSTLRAARKYMNRSLDELY"
     gene            complement(2480..3295)
                     /locus_tag="VGA21_09645"
     CDS             complement(2480..3295)
                     /locus_tag="VGA21_09645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650812.1"
                     /translation="MDIKEKIEAYFERTLTEVEMRDVEEAVSQDPELKKEFEFQEDIN
                     EALKEVRVAELKAQLESISITTSIFDQTIFKLITSIAIITGVSIIGYWALRSGNESPV
                     TTEAQNEIPLDSLDHPALIHEPDQAQAESSKEVPETTKKTTPVTTPTPVTPHVIEEFA
                     DEETREEISIPENDILENAPSASAGISVELKIGTQYNFHYQFLSNRLFLFGNFNEELY
                     DILEIKNDTESQLFFSYKEKFYQLDREQKEITPLQEITNEGLIIRLKSIKDNQ"
     gene            complement(3303..3848)
                     /locus_tag="VGA21_09650"
     CDS             complement(3303..3848)
                     /locus_tag="VGA21_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011584345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX9650813.1"
                     /translation="MKEKEIFERICRGDEKALEELYKKYYRMMAKLVITNSGSEQEAK
                     DIYQDALIVFWQKATSGNLTLTSKISTYIYSICLNLWRKELDRKKRLSNEEKDTPEYM
                     DMDMDRAERIRIIKNCINQLGDTCRKVLTYYYYEKMSMTEIASKLGFANTDTAKTKKY
                     KCKKKLDELVKSQYSEKDFLD"
     gene            complement(3996..6203)
                     /locus_tag="VGA21_09655"
     CDS             complement(3996..6203)
                     /locus_tag="VGA21_09655"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012472794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HEX9650814.1"
                     /translation="MENFIYVIPALGLIGILFMIGKSAWVSKQDAGDQKMQDLAKHIA
                     DGAMAFLKAEWKVLSYFAIIVGLLLAYSGTVVENSSPVIAISFLIGAFLSATAGYIGM
                     KVATKANVRTTQAARTSLSRALNVSFTGGTVMGLGVAGLAVIGIGSLFIIFYHVYVLN
                     FNAGVNGLEMEKALEVLAGFSLGAESIALFARVGGGIYTKAADVGADLVGKVEAGIPE
                     DDPRNPATIADNVGDNVGDVAGMGADLFGSYVATILATMVLGREIISNDNFGGIAPVL
                     LPMVIAGLGLIFSIIGATLVRVKEGGNVQNALNMGNWVSIILTVIASFFLVNWMLPET
                     LSTRGVVFTPMDTFWAIFVGLIVGALMSIITEYYTAIGKKPVNSIVRQSSTGHATNII
                     AGLAVGMQSTFLPILVLATGIYTAYQFAGLYGVAISAAGMMATTAMQLAIDAFGPIAD
                     NAGGIAEMSGLPEEVRGRTDILDAVGNTTAATGKGFAIASAALTALALFAAFCGVAGI
                     SSIDIYKAEVLAGLFVGAMIPFLFSSLAIAAVGRAAMAMVEEVRRQFKEIPGIMEGTA
                     EPEYGKCVDISTKASIREMILPGSLALVVPTLVGFAFGPEVLGGLLAGITVSGVLMGM
                     FQNNAGGAWDNAKKSFEKGVEINGKMEYKGSEAHKASITGDTVGDPFKDTSGPSMNIL
                     IKLTSIVALIIAPHISVKKHNDKAEVKSDKIEQVSTLNPNMPEEEVISFKKSN"
     gene            complement(6290..6739)
                     /locus_tag="VGA21_09660"
     CDS             complement(6290..6739)
                     /locus_tag="VGA21_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018342965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HEX9650815.1"
                     /translation="MQLEKDLKQRSFTSDYQKALINILYTHNYLINKINYFFKDHQIT
                     RQQYNVLRILRGQYPNPATINLIKDRMLDKMSDASRIVERLRNKQLITREINSVDKRS
                     ASLRISKKGLDLLKKTDDKVREFDEILKTLDSKEIKVLNKLLDKIRE"
     gene            complement(6788..7483)
                     /gene="folE"
                     /locus_tag="VGA21_09665"
     CDS             complement(6788..7483)
                     /gene="folE"
                     /locus_tag="VGA21_09665"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009583226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEX9650816.1"
                     /translation="MKRPKEIILNPSTYTASDIERYGDDHVATSIETPMRDDAFSIDD
                     DAKIKKIAHHFHEIMETLGLDLTDDSLKGTPNRVAKMYVKEVFSGLNPENKPKARLFE
                     NKFGYNEMVVEKDITFYSHCEHHFVPIFGKAHVAYISTGKVIGLSKMNRIVQYFAKRP
                     QVQERLTVQIADELMQVLKTPHVGVVIDANHLCVSSRGVGDTNSKTGTAIFKGKFSEE
                     NTKREFLNYINSK"
     gene            complement(7494..7901)
                     /locus_tag="VGA21_09670"
     CDS             complement(7494..7901)
                     /locus_tag="VGA21_09670"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020531555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HEX9650817.1"
                     /translation="MKVSVYRKEHFNAAHRLHNPSWSETRNQEVFGKCNNPNWHGHNY
                     ELVVRLTGEPDAETGYVFDLKALSDIVKAEIISRFDHKNLNLDVPEFKNCNPTAENIA
                     ITIWNLLRTKINKKFELKVLLYETERNYVEYPA"
     gene            8181..9221
                     /locus_tag="VGA21_09675"
     CDS             8181..9221
                     /locus_tag="VGA21_09675"
                     /inference="COORDINATES: protein motif:HMM:NF013292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase"
                     /protein_id="HEX9650818.1"
                     /translation="MSNRRKFIERAVGLGLSLPLFPAIAKASAQYSNPVKAAKLPLIL
                     CSRGEYWGRKVLDPAWEKLKTGSILDAIEVGANVVELDPEDNSVGYGGLPNEEGIVQL
                     DASIMYGPTHNCGSVMALEGVKTPCSVARLVMERTDHMVLAGEGAQRFARAHGFKIEE
                     LLTDRSRRIWLRWKENISDRDDWLPPADGNYGLERSTGTINVLGIDTEGNVAGITTTS
                     GLFGKIPGRVGDSPIIGAGLYVDNSVGAAGATGRGEEVIRTCGSFYVVQLMEQGKSPQ
                     EACELACKKIVDINGGPSKADVDDMFVAINVNGEVGVASIKGRKDNLPQAAYYSAEGF
                     KVVRGTYLMERD"
     gene            complement(9218..9907)
                     /locus_tag="VGA21_09680"
     CDS             complement(9218..9907)
                     /locus_tag="VGA21_09680"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX9650819.1"
                     /translation="MAKTFVIGDIHGAHKALLQCLERSGFNKDEDNLICLGDVCDGWP
                     ETKESINTLMSIRQLIYIMGNHDYWALEWMRGEFPGEIWSVQGGDATVRSYNGLPNDS
                     HKAFLENSSLYHLIDNTLFVHAGVQPEIDLNDQSSNTFLWDRKLATSALELRTAKAEK
                     QLTSFDEIYLGHTPIHAYGYNHPIKACEIWFMDTGAGWNGCLSMMDINSKDFFTSDPV
                     PSMYPEDKGRF"
     gene            complement(10118..10861)
                     /locus_tag="VGA21_09685"
     CDS             complement(10118..10861)
                     /locus_tag="VGA21_09685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650820.1"
                     /translation="MHYLTLTAILGIFAFTNSLSQINIYLASKDFNLNQWVAIDSLHR
                     KIEFLTDSIKNLDSIKVVLEIKQYNILDINSDGYLDVFYKGLPGQKSGFMINTDYELE
                     MEYEVSESIINIGRAKPWNPINFQTLIKKNNSENEVKYYIPVFRNGKLTYEVENTLLF
                     HPNLVLINENVPPVGFEILQIPELRWAATSEATNVIKMYKGGDRGFAVASSADSNRRI
                     WWLVLMDEGSQTYRVGWMLRKNLKQLPRQ"
     gene            complement(11219..11635)
                     /locus_tag="VGA21_09690"
     CDS             complement(11219..11635)
                     /locus_tag="VGA21_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013453066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA/BrxB family bacilliredoxin"
                     /protein_id="HEX9650821.1"
                     /translation="MYPEELVTPIRSDLVGVGFTELKTAAEVESHLADRKGTTLMVIN
                     SVCGCAAGAARPAIKMALSHSAKKPANLLTVFAGVDFEATEKAREYTLPYPPSSPSIA
                     LFKDGELVSFVERHMIEGRPAELIAEHLMGVFNEHC"
     gene            complement(11656..12009)
                     /locus_tag="VGA21_09695"
     CDS             complement(11656..12009)
                     /locus_tag="VGA21_09695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015266849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF59 domain-containing protein"
                     /protein_id="HEX9650822.1"
                     /translation="MEDRKDIINTDNQIENAPELKEKIIEAIKTVFDPEIPVDVYELG
                     LIYEINIYPVNNVYILMTLTSPSCPSAESIPGEVEMKVKEIEQVNDVTVELTFDPPYT
                     MEMMSESAKLELGFL"
     gene            complement(12019..12447)
                     /locus_tag="VGA21_09700"
     CDS             complement(12019..12447)
                     /locus_tag="VGA21_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020529493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HEX9650823.1"
                     /translation="MNIAEVQEQIILEFSMIGADMEMATLFVMESGEKLPAMDESLKI
                     ESNLVKGCQSKVWLSPELKDDKINFHADSNAAITKGLIYFLIRIYDGRTPDEILESEL
                     FFMDKIGMNRFIGTQRSNGFLSMIKQMKMYALAFKSKINA"
     gene            complement(12444..13655)
                     /locus_tag="VGA21_09705"
     CDS             complement(12444..13655)
                     /locus_tag="VGA21_09705"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018474277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEX9650824.1"
                     /translation="MRQLRSEFPILHQTVNGRQLVYFDNAATTQKPQRVLDALESYYR
                     EYNSNIHRGLHTLAEKATSTFELSRKKAAEFINAPEIEEVIFTKGTTEGINLVAQTYG
                     RKNFKKGDEIIITGMEHHSNIVPWQMIAEEKGSVLKIIPVSGKGEIDIEDFKKLISDK
                     TRIVSLAFASNALGTVNPVKEVIEIAHSAGALVMIDGAQAAPHLSIDVQDLDCDFFAF
                     SAHKMYGPTGVGVLYGKRVLLENMPPYQGGGEMIRQVTFKKTTYNDIPYKFEAGTPNI
                     GDVIAFDESIDFILEHGRNSIAAHEEELIQFAHEKSKEIEDLVIYGEAAHKVSVFSFL
                     INGVHPLDLGTMLDAQGIAVRTGHHCAQPLMDAFKIDGTVRASFGVYNTREEIQIMFD
                     AIREIIKKLKK"
     gene            complement(13691..14938)
                     /gene="sufD"
                     /locus_tag="VGA21_09710"
     CDS             complement(13691..14938)
                     /gene="sufD"
                     /locus_tag="VGA21_09710"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEX9650825.1"
                     /translation="MELANSNNIFSKSDFKEIRVMANDYLAAKGLPDKKNEEYKYTSI
                     STLIEKTFGHTLTPTSKSELSAVSEFLIPDFDCYFLVLVNGKWKKELSNYPAELQVND
                     QPDNAQIGTIAKIENNGFVALNALHFDHCLSIKIAESSQVNKPIFIIHISDVQEENSI
                     SMPRILIDLGEQSKSSFYVGYFSKGDYTSFTNAVVEVFSGKASHSRLTQIQNESNAAI
                     THTNIYVQQSRDSNFNSICVSLGGKMVRNNLNVILDDENIESHLYGLYQLTDNQHVDN
                     HTVVDHKKPNSVSNELYKGILDEKSTGVFNGKIYVRPSAQKTNAFQSNKNILLSNDAA
                     VNTKPQLEIWADDVKCSHGCTTGQLDEEQIFYLQSRGISKDMALKMVLKAFQHDILSR
                     IEVKAVVDYLDKEIIRRFGGENQ"
     gene            complement(14938..15687)
                     /gene="sufC"
                     /locus_tag="VGA21_09715"
     CDS             complement(14938..15687)
                     /gene="sufC"
                     /locus_tag="VGA21_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018618458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEX9650826.1"
                     /translation="MLAIKNLHASVNGIKILKGINLEVKSGEIHAIMGPNGSGKSTLA
                     AVLAGREDYDIDEGSITFLGEDLMEMSPEDRARAGLFLAFQYPVELPGVSSTNFLKTA
                     LNQIRKHRGQEPMDAVSFLTLVKEKMKLVDIDQSLLSRSLNQGFSGGEKKRNEIFQMA
                     VLEPKLAILDETDSGLDIDALKIVANGVNKLHSPQKATIVVTHYQRLLDYIIPDFVHV
                     LYDGKIVKSGGKDLAFQLEKKGYDWIKMEAV"
     gene            complement(15716..17161)
                     /gene="sufB"
                     /locus_tag="VGA21_09720"
     CDS             complement(15716..17161)
                     /gene="sufB"
                     /locus_tag="VGA21_09720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014773920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEX9650827.1"
                     /translation="MSKDSHILEEFTSKEYEHGWNIDLENDSAPKGLNEDIVRFISTK
                     KEEPQWLTEWRLKAYRNWLKMEVPKWANVKFPVINYQDIIYYSAPKQKVKPKSLDELD
                     PELRRTFEKLGISLEEQKRLTGVAVDAVLDSVSVATTFKEKLMDLGIIFCSFSEAVKK
                     HPELVQKYIGSVVPPSDNYFAALNSAVFSDGSFCYIPKGVRCPMELSTYFRINAANTG
                     QFERTLIIAEEGSYVSYLEGCTAPMRDENQLHAAVVEIFAAKDAEVKYSTVQNWYPGD
                     KDGKGGIFNFVTKRGICSGENSKISWTQVETGSAITWKYPSCILKGDNSVGEFYSVAL
                     TNNRQQADTGTKMIHIGRNTRSRIVSKGVSAGYSQNSYRGLVKIMKRAVNARNFSQCD
                     SLLMGDKCGAHTFPYIEIGNSSAKVEHEATTSKIGEDQIFYCNQRGIDTENAVALIVN
                     GYAKEVLNQLPMEFAVEAQKLLALTLEGSVG"
     gene            17322..17576
                     /locus_tag="VGA21_09725"
     CDS             17322..17576
                     /locus_tag="VGA21_09725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650828.1"
                     /translation="MRNDSKNIYTSVIGGYFDEEFEFYTKLFFEKVEQGIFKMILSDI
                     LATELNGAPEDVLKFYQSIPKNQIEYIDQTEDSIREGKNT"
     gene            18195..19856
                     /locus_tag="VGA21_09730"
     CDS             18195..19856
                     /locus_tag="VGA21_09730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009194362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX9650829.1"
                     /translation="MADFPWYKHYPKGVAQEINPDQYSSLVEMLDEASKKFSSSPAFV
                     NMDKRISYKEMKEKATELAAFFQSIGLKKGDRIVVQMPNLLQYPVIIYGALKAGLIVV
                     NTNPLYTPREMEHQFKDSGARAIIILANFAYNLEKIIQKTNIETVIITEIGDLLGGFK
                     SMVVNFAVKKIKKMVPDYHLPGAIKFNVALKSGRSKKFTPVELIGKDVAFLQYTGGTT
                     GVSKGATLTHRNIVSNILQSKEWFVGLKEKEEVVITALPLYHIFALTANFFNMYMIGT
                     LDVLITNPRDMKAFIKELKKYPFTVFLGVNTLFNGLLHQPDISDVNFSHLKIAVAGGM
                     ALQKPVATLWKDKTGNSIAEGYGLSETSPVLSCNPLNGTERIGTIGIPYPSTEMKIMD
                     ENGNEMPLGEPGEICARGPQVMPGYWNREDETAKCFFGEWFKTGDIGVMDEDGFFSIV
                     DRKKDMILVSGFNVYPNEIEDVISGHPKVLEVAAIGIPDVNSNEAVKVFIVKKDESLT
                     EQEVHDYCVENLTGYKRPKAIEFMKELPKSNVGKILRKNLREAVS"
     gene            complement(19853..20617)
                     /locus_tag="VGA21_09735"
     CDS             complement(19853..20617)
                     /locus_tag="VGA21_09735"
                     /inference="COORDINATES: protein motif:HMM:NF024946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEX9650830.1"
                     /translation="MKKKFLVLIMISMAGFTQAQDIGSIFTDRPSQTDAVALLPKNVF
                     QIESGYFWSNDEVLGNATTSMNIPNLMLKYGLLPWLELRAGTALLYQKQENISILTTN
                     TELGGIFIAPKLKILEPKGLIPRISATAYLTLPGTGTGFAKDNNGALNTRLLLENPLS
                     DNLSLAYSIGTDSDFSGNTNGFYSIMLSASFGRLGAFTEFFSNVINGGPNAVGLDGGV
                     GFTLTDNFTIDLAIGLGLNTYATDIFLNSGIGYRIH"
     gene            20752..21855
                     /locus_tag="VGA21_09740"
     CDS             20752..21855
                     /locus_tag="VGA21_09740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009580389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650831.1"
                     /translation="MKRKIIETLLIISILPGCSGSSNEEQQSDELAAVDTLVYEGEKN
                     LTNVIQLTFGADNAEAYWSFSGEMITFQSNNSEWGVNCDQIFFTEVSDANPVTQKPQM
                     ISNGLGRTTCPYFMPGDSTIIFASTHPGGNDCPPEPDRSSGKYVWPVYSSYDLFIADL
                     KGNIITRLTDEPGYDAEATLSPKGDKIVFTSDRSGDLELYIINTDGRGITQITNELGY
                     DGGAFFSPDGTKLVFRASRPKTEEEISEYKNLLSQGLVEPTMMELFVCNIDGSNMRQI
                     TNLGKANWAPFYHPSGRKIIFSSNHHGSIGFQFNLFMINEDGSGLEQITFDPVFDSFP
                     MFSPDGKRLIFSSNRNNKGTRDTNLFIAEWVEN"
     gene            complement(21852..23234)
                     /locus_tag="VGA21_09745"
     CDS             complement(21852..23234)
                     /locus_tag="VGA21_09745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013452815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HEX9650832.1"
                     /translation="MNRVGFSSRIGFVAAAAGSAVGLGNIWRFPYETGQHGGAAFLVV
                     YLICIIIIGYPVMVGEISLGRSAQANPYRAYRSLGTKNWGLLGIWGIICGIMFLSAYN
                     VVAGWAFGYFLQISFGDLLSTSDLPGKFGTYIADISDNFFYSLGFMVLTAIIVAGGIK
                     KGIETASKILMPLLFLLLIGLIAYSLTLENAMEGVRFYLIPDFSLINVRTIYSALGQA
                     FFSLSLGMGALITYGSYISKKENIGNSAGMVVFTDTMVAFLAGLMIFPLVFSQGQTPT
                     QGPGLVFVALPGIFAQMGLITGKIVGGAFFLLLCFAALTSTISLLEIPTTYLVDEKKW
                     PRLRVVILSATVIFLVSLPSMLSHGAIDFFTRFLHYEGVDKSFFDLVFDVFSDVGLPL
                     GGFLMMIFINKKWGMKSFDEEMTQGNSNFVTGILKTFLHFMIIWVNPVLLGIMVVVTV
                     LQKFFGIIIF"
     gene            complement(23303..24631)
                     /locus_tag="VGA21_09750"
     CDS             complement(23303..24631)
                     /locus_tag="VGA21_09750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009578147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HEX9650833.1"
                     /translation="MDVLDFITLLIIMAAFFTLVNVKYLKLPSTIGLMILALCLSLFI
                     IFGELVFKTLNTLAQDLMTKYNFSEVLFKVMLSFLLFAGAIEMDLRKLKEEKWVIGIL
                     AVFGTLISTFVVGYAMYFLLPLVNIQLELIHCLLFGALISPTDPIAVLAMIKKSHVSK
                     NLESQIAGESLFNDGIGVVIFLTILSIAFPRGGLAEAQISAMHVAELFGVEVVGGILL
                     GAAIGYVGRWLLVIIDNEHTEIEVLVTLSMVLGGASLAEMLHVSMPLSTVVMGIFVGE
                     KGRGEETDPIAGEYVYKFWSLMDEAMNAILFILIGLQVIVINWELGFFVAAGIAIIIV
                     LLARFIGVSIPISIIRTRIEIPKYTIRILTWSGLRGGISVALALSLYDTAKEFSVNPR
                     IIDLIISMTYSVVLFSILVQGLTVGKLFAKANPDNGSAPTSEKPEVSDHT"
     gene            24847..26268
                     /locus_tag="VGA21_09755"
     CDS             24847..26268
                     /locus_tag="VGA21_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019941540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX9650834.1"
                     /translation="MGTYRNLTTKEKALQINLDSSIYGSFAEIGAGQEVAANFFKAGS
                     ASGTIAKTISAYDMTFSDAIYGQCERYVCEPRLMGMLGKEFNLLRKRLSRKADKKKFF
                     AFADTVETSPFGHMRDGHGWIGLRFQTSPKREPNECIIHVHMLDDDNVLQQQTLGIVG
                     VNLIYGCYFIDDPEALILSLMDDLTSRRIEIDMFKLEGPDFQHVDNRLMSLKLVKNGL
                     TKAAMFGPDGEVLQPSEVLYKKNVLVLRGRFRPVTHVNIDMLLSGRREFKKDPDVDKK
                     DILVISELTLQDLSNEGEIDDLDFLSRVDIICSLGQNVMISNYYEYYRLVDYLSLYTR
                     KKKIGIILGFNNLARVFDETYYENLRGGILESFGTLFGHNVTLYVYPCRNQDSNEICT
                     TKSFNVPRHLRGLFDYLMDNHKLQDVETANLELLDIISDNVLIKIQRGIAGWEETMPT
                     KIALAIKEGGLFGYKAPAEQQIT"
     gene            complement(26274..29804)
                     /gene="smc"
                     /locus_tag="VGA21_09760"
     CDS             complement(26274..29804)
                     /gene="smc"
                     /locus_tag="VGA21_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009185578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEX9650835.1"
                     /translation="MQLSRLEINGFKSFGDKVTINFDEGVTGVVGPNGCGKSNIVDAI
                     RWVLGEQKIKSLRSDKMENVIFNGTKKRKATNLAEVSLTFNNTKNLIPTEYSQITITR
                     RYFRTGESEYLMNGVHCRLKDINNLFMDTGIGSDSYAIIELKMVDDILNDKDNSRRNL
                     FEEAAGISKFKQRKKETLRKLQDTDADLERVEDLLFEIDKNLKSLEKQARQAERYYKL
                     KEEYKEFSVELARTSIGKQNNTFKELSKSIENENDRKLQLNNKIIEIEAEIEKLKTDL
                     ISKEKELSVRQKALNNHVNEIRTYENEKQIKNEQLRFLNNKSDMLREQIEQDRKSKDR
                     AAFSIQSLNQEKEEVQRVLGEIGKQVEVLHKDYEDLKELTNQLQEEVNSLNQTLRSRQ
                     DEVYQLKKSIEIREIQINSFTQELEKTSTDSSKRSENLIEFEQKIAGLTFQISDLNTQ
                     KEQLKEDEVNLESRIENSGKTIEMIRDEIVQVHRKLDARQNEYNLTKSMIENLEGFPE
                     AIKFLKKEAGWGKKVPLLSDIISCGEEYKVTIENFLESWMNHYVVEQPEEAYRAINLL
                     SDSAKGKANFFILNLFEKFKPSSTQIFDNAFPATEILEFDPKYKKLVSFLFDNVFIVI
                     GDYKEIPNHEDCIFITKNGKVTRKKFSISGGSVGLFEGKRIGRQKNLEKLEIEINSLN
                     TKLDEAEQSRRDSEAQLESLINATHKEEIEELQNQINLLNEELISLKTKHEQTRELLS
                     STDTRKEDMIDKINQLKQEIIELQPKTENEGQELGEMESKLKLLTINLKEQNAILNEK
                     SAAYNEENLHFHQQQNKVNSIDQELKFKESSLESSSERINKNSEELQLNEQDIQVLIA
                     NTASQDDELVKLYKEKEEKDRLVNEAEKDYYEQRGKIDDKEKEEREAHKAKEMVDDLL
                     LELQNKLNETKLELNSVKERLSIEFNINLDDIMQQELPKVEDEEVDELKGKVTRTKER
                     MEKMGPINPMAMEAYQEIQKRYEFITTQKQDLINAKESLLATIAEIDLVAKNTFLDAF
                     EKIKGNFVKVFRSLFTEEDDCDLILSDLENPLESSVEIIAKPKGKKPITINQLSGGEK
                     TLTATSLLFAIYLLKPAPFCIFDEVDAPLDDANIDKFNEIIKEFSKESQFIIVTHNKR
                     TMVYTDVIYGITMIEQGVSRVVPVDLRELA"
     gene            29873..30376
                     /locus_tag="VGA21_09765"
     CDS             29873..30376
                     /locus_tag="VGA21_09765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650836.1"
                     /translation="MDDSLQIIIYIVFIAIYFLSRFLKGNKQKASRPRPRQESEEEGQ
                     VETNEQPMTFQDILRELTQPREAPAPQPKKRAPVEPGPRDYEFKGDYPDEAEIQEVYE
                     ESLSRAKNVKTLDEMINIGEESIKFKEYQEADQQNALATEIREMLKDPYDAKRAIILK
                     EILDRKF"
     gene            complement(30384..31001)
                     /locus_tag="VGA21_09770"
     CDS             complement(30384..31001)
                     /locus_tag="VGA21_09770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013929786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HEX9650837.1"
                     /translation="MFCYGQEQLKIAKLKYSGGGDWYANKTALPNLAKFCNQNLNMHL
                     ALEEDIVEVGSSELFLYPYIYMTGHGNVVFSPPEAQNLRKYLIAGGFLHIDDNYGLNQ
                     FIRLEMKKVFPELEFVELPFDHPIYHQKFDFDQGLPKIHEHDGKPPQGFGLIYKGRLV
                     CFYSFESDLGNGWEDQVIYNDPEEIRQKALQMGANIISFAFTHIQ"
     gene            31083..32435
                     /locus_tag="VGA21_09775"
     CDS             31083..32435
                     /locus_tag="VGA21_09775"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HEX9650838.1"
                     /translation="MSFSFNAIYNNYSEFRENSLHGLQVKAEEIRPLLEKAKNVNGIE
                     VSEIGKSFLGESIHKIVIGSGSKKILAWSQMHGNEATATKVVFDLVNLISKRPELTGL
                     IFSKLQLTVIPMLNPDGAKSFTRINAQGIDINRDAYARLTPESKILHKIFAELKPEFC
                     LNLHDQRPIYNVGESATPATLSFLAPPYNKEVEVNEERLIAIKMVVALEKLLTQELKI
                     GISRYSDTYERRAFGDTFQSKNVPTVLFEAGGYRGDHDRELVRSFYFMALLSSLHFLA
                     EDSFLHEDPDDYFKIPVNEELLFDWIIRRVKITDSGKEWIADVGGQREYSGSKYGKGI
                     IEEVGDLSQYYGYTEIDGSGCILEKGKVLVSPDENNKDTQELVAKGYTTIQSESFPAG
                     HSLAINIQRSAREFLITPGHPANLVVFRNKKIVKVILNGFILDPSRRPFENYYGISLE
                     "
     gene            32486..33553
                     /locus_tag="VGA21_09780"
     CDS             32486..33553
                     /locus_tag="VGA21_09780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020532887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1611 domain-containing protein"
                     /protein_id="HEX9650839.1"
                     /translation="MKSNAIIITAGILDDIHAKTTHGLVRGTDKFNIVGVIDSNHAGK
                     DAGEVIDGKKRNIPVFRDFEDFRNKSPQKAELCIIGVATKGGRIPAEMRDVIKEAISS
                     GYGIVNGLHEFVSEIKELKELAGEKGVELIDIRKPRPRNELRFWSGEIYRILSPIIAV
                     LGVDCAVGKRTTSRMLVQSMKDVGINAEMIYTGQTGWLCGIKHGFIFDATYNDFISGE
                     VEAALLSCYDDQKPEVIFIEGQSGLRNPSGPCGSEFLLSGNAKGSILQVVPHRKNFKG
                     FENLNCSLPSATDEIALIKMYNSEVIGITVNTEHLTKEESFQAKTQLEKETRLPVALP
                     LFEGVEPLVPALVEYVKNYKR"
     gene            33550..34614
                     /locus_tag="VGA21_09785"
     CDS             33550..34614
                     /locus_tag="VGA21_09785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020532475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEX9650840.1"
                     /translation="MKITRYSFWRQNLELTRPYAIAFKTTDSVENIFFEVELADGKSG
                     LGCCNVSAKVVGVTLDDCWKFFNNEDLNWLVGSDIREIGRLQAELVNKYHSNPGVRAA
                     MDIALYDAFGKYLDIPLVNFFGRAIESLPTSVTIGIKNVEETVKEAEEYKGMGFTNLK
                     VKLGHSVNEDIERLIKLNEELPEMVIRVDANQGYSLNDLQFFLEKTRALNLELVEQPL
                     PVDQVDEQIKLSLEDINVLAADESLVGPEDVATLIAPQPLFGIFNIKLMKAGGIYQAR
                     KIGEVAGVNDIPLMWGCNDESIVSISAALAAAYSYPWTKYIDLDGSFDLAKDVVKDGF
                     ILKEGRMYPTDKPGLGFTRL"
     gene            complement(34609..35205)
                     /locus_tag="VGA21_09790"
     CDS             complement(34609..35205)
                     /locus_tag="VGA21_09790"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002693783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HEX9650841.1"
                     /translation="MKKYSVGIFLFDDVEVLDFAGPFEVFSVTSQLNNYEPFSVFTIS
                     ETGEEINAVNGLNILPDFSFEDHPDIDILIIPGGDGTRELINKQLVIDWVNEISHGSE
                     ITMSVCSGARILAKAGLLDGHNSITHHEVIKDVKNITSETNILEGKRFVDEGKIMTSG
                     GITAGIDLSLHVVEKLFGKSMARETVIYMEYGDWRKLL"
     gene            complement(35286..38540)
                     /locus_tag="VGA21_09795"
     CDS             complement(35286..38540)
                     /locus_tag="VGA21_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002698533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650842.1"
                     /translation="MKLRNLFTILMMILLSSELSLGRQLEVSKFKDMKARSIGPAGMS
                     GRITAIDVVASDPKIIYAGSASGGLWKSSSGGIAWEPVFDKEAVSSIGAITIQQNNPD
                     VIWVGTGEGNPRNSLNGGYGLYKSLDAGKTWTLMGLEKTRNIHRIIIHRDNPDVVFVA
                     SIGSPWGDHPERGVFKTKDGGKTWEKVLYVDQKTGAADMVVDPTNPNKILVAMWEHRR
                     KPWTFTSGGPSSGLYVTLDGGTTWTKQSTENGLPEGDLGRIGLAIADSKPNIVYAIVE
                     AKKNALYRSEDGGYKWSKVNDEPVIGNRPFYYNDIFVDSRNENRLYHLYSSVDVSEDG
                     GKTWSRLMQGNIGGIHPDHHAWYIHPENPGFMIDGNDGGLNITHDGGKTWRFAENLPV
                     GQFYHVNVDNQLPYNVYGGMQDNGSWGGPAYVFKSGGIRNSYWQEVSFGDGFDVVPDP
                     ENPRYGYTMSQQGNVRRYDIQSGHTKVIKPTHPDPGVQLRFNWNSAIAQDPFDKSTIY
                     FGSQFVHKSTNKGSSWEIISPDLTTNEPEKQKQHESGGLTMDATGAENYTTVLAIEPS
                     AVEKGVIWVGTDDGNVQLTRDGGKSWINVAGRITSMPKGAWVCQVKASNFSGGEAYVV
                     ANNYRNFDFKPYLFRTKDYGQTWESLVSENTVWGFSLSVVQDPVEKRLVFLGTEYGLY
                     VSIDEGKTWSKWTNGYPTVPTMDLVIQPREHDLVIGTFGRSFYVLDDIRPLREIASKG
                     TQLLDHTVKLFPIPEAYMTVSQTPSGLRFTADAMFSGQNRDNGARISYVFNKPGSNES
                     PVASSNQSARERRGNNNATTNLTPESPKADNKKADSLIFEFFNEGGKLVRTIKRKAPE
                     ENGVYIYNWNLREEGPEFPGRGERRGGFGPPSGVDVLPGNYKVRLTLGDTKDSATVKV
                     NFDPRLEVSESELKAIYDAQKELDSYIALASEVTSRLKESISLVDDYSRRMRSEDAIK
                     FKQQLDLCKTTRESLNGFMDAMVGKVDNRQGITRNPEPTVTGYIFTARGYLRSTLDAP
                     GETEKRTMEHANTKLMELVNKVNSYYDTEWSVFRSEIEKVEFSPFTDLEPLKKK"
     gene            38716..38970
                     /gene="yidD"
                     /locus_tag="VGA21_09800"
     CDS             38716..38970
                     /gene="yidD"
                     /locus_tag="VGA21_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013408823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEX9650843.1"
                     /translation="MSKFLRQIFILPVRFYQVAISPYLGANCRYTPTCSEYTIEAINE
                     WGPMKGLWLGIKRVASCHPWGGHGYDPVPGKEEIKSTKQT"
     gene            complement(38954..39670)
                     /locus_tag="VGA21_09805"
     CDS             complement(38954..39670)
                     /locus_tag="VGA21_09805"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HEX9650844.1"
                     /translation="MNRWYENRNHDIHSNGEVWLLKKIANLNPKVIIDGGANVGEYAL
                     EATKICTSASVHCFEPVPDTSDLLKKAIGNYSSNVIVITKGLSDVNQQIPMNLFEYSV
                     HSSLYSHNGITSETKTIIDIEVIKGDDYVHSIGIEKIDLLKLDIEGAEMKALKGFENC
                     LREKRIRVVQFEYGYLNILTKNLLMDYYDFFEPLGYRVGKLYPKKIEFKPYSNKLEDF
                     IGPNYVAVLETEIEFIRSVS"
     gene            complement(39777..40727)
                     /locus_tag="VGA21_09810"
     CDS             complement(39777..40727)
                     /locus_tag="VGA21_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020528019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEX9650845.1"
                     /translation="MSRQVALGIDIGGTDTKYGFVEKSGEILSKGKFSTNTNDDIIFF
                     IADLKKEIKKALAALPEQIELIGIGIGAPNGNFYRGTIEQATNLAWKGIVPLSRLVEE
                     SLNAKTFVTNDANAAAMGEMLFGSAKGMRNFVVITMGTGLGSGIVVNGEIVYGHSGFA
                     GELGHQTVNINGRSCACGKKGCLETYVSATGIKRTVYKLLADSREESVLRNIGFNDLS
                     AEMITGEALRGDKIATEAFEYTGRILGIKLADTVYHLAPEAIFLAGGLAKAGSILFDQ
                     VIFQLEKNLMTLFKDSVKVLPSGLEDQDSSILGAASLILK"
     gene            complement(40732..41712)
                     /gene="pfkA"
                     /locus_tag="VGA21_09815"
     CDS             complement(40732..41712)
                     /gene="pfkA"
                     /locus_tag="VGA21_09815"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013455200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEX9650846.1"
                     /translation="MSKIKKIGVFTSGGDAPGMNAAIRAVVRAGIYYKFAIVGIFSGY
                     QGMIKGKMKKMNVRSVANIIHRGGTILQSARSEEFRTRTGRKKAIEKLKKNKIDALVA
                     IGGDGTFRGAITLNKEYDIPIIGIPGTIDNDLFGTDATIGYDTATNTAVKAIDMIRDT
                     ALSHNRLFFIEVMGRDSGFIAMRSGIAGGAVATIIPEDDITLEELVEIINKGLKSKKS
                     SSLVVVAEGGKLGGALQLAEKVKEKFSYFEPRVTILGHIQRGGSPTVFDRLLASRLGV
                     AAIEALKEGKSNVMAGIKNSKIVFTPFQKSISTPKIINQEAWRIAKILSS"
     gene            complement(41735..43180)
                     /locus_tag="VGA21_09820"
     CDS             complement(41735..43180)
                     /locus_tag="VGA21_09820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018473073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX9650847.1"
                     /translation="MKSLHRKKFNRRDFIKGSSLAAAGFIIVPRYVLGGNGFISPSDK
                     LNIAAVGAGGRGAGVINEMYKEGLNNIVALCDVDDRQSANTYNKHPNAPKYKDFRMML
                     DKQKDIDAVTVAIPDHSHAVVALSAMASGKHVYVEKPLAHDVYEVRMMTEMARKNKLA
                     TQMGNQGASGAGTWQTVKWIESGVIGEITNVHCWTNRPIWPQGIPTPTEKHPIPAELD
                     WNLWLGPSKMRDYNSGYLPFKWRGWWDFGTGALGDMACHIVDVVVRSIKLGHPDSVEA
                     SVTEQYVADFKEAIFDDSCPMACKVVFHFPDRGTGLPPVKLTWYDGGILPDRPAELGP
                     DDPLGSWDGGVIFEGTKGKIMTECYGNNPVLLPKSLMEENAAPSDETPVKDGHYQSWV
                     NACKGGEPAVSNFDISGPLSEIILMGNLAIRSFGIRELKPGRKYGDWAPFNYPGRKQL
                     MWDGANMKITNFDEANQFVKREYREGWNLPV"
     gene            43330..43662
                     /locus_tag="VGA21_09825"
     CDS             43330..43662
                     /locus_tag="VGA21_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEX9650848.1"
                     /translation="MKQEGLGEFQELILLAILVLEDDAYGVSIQKEIKDKAKRKVSRG
                     ALHSALTRLQEKGLVVSKMGGATAERGGRRKRFFNLTQKGTRAVTLARISRVNFYQAI
                     GNLDFGKA"
     gene            43662..46277
                     /locus_tag="VGA21_09830"
     CDS             43662..46277
                     /locus_tag="VGA21_09830"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9650849.1"
                     /translation="MKKGTPPLWARKFLQWFADPNYYEELQGDLEENYFRDIKSLGTL
                     KARWNYIFETFKLFRPSVWNHSFFTSQNSNYMFRNYAKVTWRNLRKQKIFSFINLFGL
                     AVSLSVCLLIISIIVDQLSFDKFHKKYANIHRIITYNNDLRYGWDEYLATTSLPLYEN
                     LKNNPVVGDIVRIKRNFWGDVETGKKTIPLSGHMADKDFFKIFSFELISGNPENALAA
                     PFSVVLSESSKYKLFGDSKALGETIKVGRYGDYIVTGIMKDPPRTSHMQFDMIGSFAS
                     LEGLKDSGIYAGNDNWLNYTEYYIYITIPDKSETETLENLLHRFGEVESKKINNRILD
                     FELQSLAAITPGRDLANQIGPNFGIAPLRSLMILALLILSLACFNYVNLTLAKSLKRF
                     KEVGIRKVMGSQKRQLVIQFVFESVCLSLIAIIPAFVLMVALKPQFFSIEPSSMARFL
                     TFEFTPEIILAILGFTILTGLLAGIIPALILAGVKASAGLKDKTVKIFTGISLRKGLV
                     VAQFGIAIFFIILTNLAYKYYNFQLDFDLGYAYDEIYNIELQGTDHEIFRAEFSQIPE
                     VESISFSNYLLSTSTKNSNLIITENRQDTIRSVNMAIDENFLSNHNFAIIAGNDFNDF
                     TLGNENRIIVNEKMVLAIGKANNADVVGLSVWLEGKEVSIAGVVKNFYHNRSSQPIEP
                     FFFKYNPDNVHFANLKLRSKNLPATISKMETAWKRIDREVHSYKGEFYSRQIEASLSE
                     LKAMIKLIGFSALLTICISLLGILGMIIYTCETRVKEIGIRKVLGASISTVVYTLSKG
                     YITILSVASIISLLLVYFLYSKIISPELNTKPDFTFLDYFGGVLAVSGIGLLIVIFNG
                     YLTALRNPVISLKEE"
     gene            46483..46812
                     /locus_tag="VGA21_09835"
     CDS             46483..46812
                     /locus_tag="VGA21_09835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEX9650850.1"
                     /translation="MKGTNLGEFEELVLLVTAGLGESAYTVAITDQINGSTKRSVVLS
                     VVHTALHRLEKKGFVKSTLSGATEERGGRRKRVFTITSAGMKALSNSKSQRDQLWELI
                     PEINFQK"
     gene            46824..49439
                     /locus_tag="VGA21_09840"
     CDS             46824..49439
                     /locus_tag="VGA21_09840"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9650851.1"
                     /translation="MKREVPKWADRFLQWFCEPSMREDIQGDLYEYFNRRVESDGIRM
                     ARIKYVLDVFRFFKPHVVRKPKFHYSNPFNFMKFNFVLALRSFLKSRLIGILNLVGLI
                     TGITCAVLISLWVISELSYDSFHAKSDRIFRVPNTFTSKSETFTQAISGPALGAQIPG
                     LISGVEAGTRTMGIWSQIGIDDKQFVEKGVLIADPNIFEVFSFKILKGSAREALTELN
                     SIVLTPEMATKYFGTENPINKTLVLGGERAMKVTALVETPPPNSQLQFDFVIPADLVK
                     DFWSWENMDETWIGGAFHTYVLLNDGVNPNAIEKEINDLVWEKSGERQRTMDVKYNYF
                     LQPLTSIHLHSDLRYDHSNNGGMENVYIFSIVGLAILLLASINYINLIVAGSITRAKE
                     IGIKKVLGAMRSQIAGQHLLESVLSVLVATAISLGVVYLLLPFFETFTDSQYGWLLKG
                     EFFLSIIAGSLILGILSGLYPAVILSRYHPSETLKGNFKKGAKGMWLRKTLIIFQFAV
                     TITLLISILVINRQMKFINSQNLGLNHKEILYVNIENSDPLQENFQAFRNELMNISGV
                     INVSGHRRAYPVNGLSNGITMVENSNGEMVSSSLYHMFVDYNYADTYGLKLLAGRFFS
                     EQFSSDSTDAIVVNLAAVKTFGWGEPENAIGKKFGEAPEHRFVIGVVNNFNFETLRKN
                     VEPLRILPVNTPTEVSIKAEFSNPAALISGIEKVWKKFLPGQAMNPAFMDNDLQNQYT
                     TELRFKTIFAIFAGISIIIACLGLFGLATFSVQQQTKEIGIRKVLGASTAGILRMINQ
                     QFLLYVVAALLFAIPLAIFLMRKWLNGFAYKISLEISDFILGATIALIVAVVTISYHS
                     LMAATTNPVNTLKAE"
     gene            complement(49562..>49918)
                     /locus_tag="VGA21_09845"
     CDS             complement(49562..>49918)
                     /locus_tag="VGA21_09845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009581265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HEX9650852.1"
                     /translation="LGLYCVGFTLFAFATQGWMMFAFLIPYALGGIAGPSLQGIMAGQ
                     VNSNEQGELQGALTSLISVTSIVGPPVMTNLFAYFTSSASSIYFPGAPFLMGAVLSLI
                     SSLLARRSLKRTLSAN"
BASE COUNT        14609 a        10137 c         9846 g        15326 t
ORIGIN      
        1 aaattacttt aaaagcaatt aacggaattt ctattttaag tatttcaaaa ctgtatagag
       61 ttacttttct aggctgaatc gattgctttt ctcctttccg ggccttcagg taagcccttt
      121 tgccttcaac ttttatggcc gaaaatgccg gtggtatctg gtctactgtg ccaacaaact
      181 ttttggcagt ctcaataact gaatcaggag taacattttt gcaattggat acgaatttaa
      241 ccggagtttc aagatcaaag gaatcggtta tttgacccag agtgaattca cctatgtact
      301 ctttctccat ttcctgaatc tcattaattt cctttgtctt ttttccggtg caaagaataa
      361 gtaatccagt tgccagtgga tcaagagtgc cggcatgtcc taccttcctg attcccagac
      421 aatttctgat tttctttact acgtcgaagg aagtccagcc agtgggcttg tcgatcagaa
      481 tgattttgtc ttgttcttca acactcatct aaaaccggta atgaaaaatc aaagcggaca
      541 tcccaagaat gaagcagtag atcgcaaagt acaaaagctt cccgcttttt acaattgaca
      601 gcatccattt gcatgcgatg tatcctgaaa taaatgccgc aataaaacct attagcaaag
      661 gttgaattga ataaccttct tcaagccacc caccggaaaa tattatttta gcatttgcgc
      721 cgataattgg aagaaggacc ataagaaatg aaaatcgtgc agcttcttct cttgaaatac
      781 ctaataacaa tgcagttgaa attgtggccc ctgatcttga tatacccggc aatattgcca
      841 ctacttgaac caaaccaata attatggagt gaaaataccc tatcggcttt tctcttgatc
      901 ggttgaaatg ggcaaataca agaatgagtc cggtgataag gagcataatt ccaacaatcc
      961 caatgttacc aaaaaataaa gctttgattt cactttcaaa gaccaagcct acaatggcga
     1021 tgggaatagc ggagatcagc aattttatta aataggagga ttgctcgttg tttcccttta
     1081 gcgaaattcc tgaaagaatg aacttaattt cctttctgaa aacaactatg gtactgagga
     1141 cagttgcacc atgtagaatc accgtaaaaa gcaattcatt tcctggatgc agcccaaaca
     1201 aagcctggcc aatttcaaga tgaccgctgc tgctaattgg aagaaattca gtaagtccct
     1261 gaaggacccc aagaataaag gcctcaaaat ttgtcattta tttcctgggc cggtagagga
     1321 tagcaaagaa ttcaagcaca aatccactca taactacaag agggccaata gttaagccta
     1381 acactccaaa tccatgtgga tctttatcga gcgacattat ggtaaatccc agtgcaatag
     1441 ctaagatgcc tacgatcaat agaatataat ttattcgctg aaatgctaag ttttcctttt
     1501 tactcatttt aataaagttc gtctaaagag cggttcatat attttcttgc agcccgcaag
     1561 gtactaaaaa aacccagtac aataccggag agtataaggc ttccaagtaa gatcataatc
     1621 agtctctgat cccttaatgc taccagctcg tctattgttt tgtatgaata attgagcaca
     1681 accaggatgg caactgaagc aaaaacaccg cccagaaatc catggaaagc agatcggaga
     1741 agaaatggtt tcagaatgaa tgctttcgtg gctcccacca gttgcatgct tctaattaag
     1801 aatctctgag aaaacaaggc aagttttatg gtatttccaa taagtataat cactaccaca
     1861 accaggataa aagtaaagcc caccaagaca agtccaatct ttgttatatt ctgatttatt
     1921 gaagagatta aattatcaac atattctact tcaaaaaccc cggaaatgtt atttatttct
     1981 gacctgatag aatccatttt gaattccgat tgatattttt cattcaggtt tatgataaat
     2041 gcatcccgaa ggggattctc gcccaggaag tcaaaatctt cacctgtgtt ttcaacaaag
     2101 tccttagcgg cttcttcttt tgaaataaat cttatccggg gttctccgct atttgttgca
     2161 acgaaatcct gagatgcgag tgattgcctg attgatattt gctgattttc cgaaagattc
     2221 tttctgaggt aaacctgaat ttcaacattc tccctgatga gagtagctag tttttgagaa
     2281 tggatgatta aagatgcgaa taatccaaaa acaaaaagag caagggtaat actgaacaca
     2341 acgcttatat agggataact ccccagcttt ttcttctttt ttgttttgat tggtttttcc
     2401 actttaacct atatatgctc aaatttaagc taaagataga tttgggcagc aaataaagaa
     2461 aaaaattaag atttaccggt tattggttgt ctttgataga ctttaacctg atgataagtc
     2521 cttcattggt tatttcctga agtggagtaa tttccttctg ttccctatca agttgataga
     2581 atttctcttt ataggagaaa aacaattgtg actcagtgtc gttttttatc tccaaaatgt
     2641 catagagttc ctcattgaaa tttccaaata ggaaaagtct gttactaagg aactggtagt
     2701 ggaagttgta ttgtgttcca atcttcaact ccacacttat gcccgcgctt gcggagggtg
     2761 cattttcaag aatatcattt tcgggaatag agatttcttc ccgcgtttcc tcatcagcaa
     2821 attcttcaat tacatgaggc gttactggcg ttggagtagt aactggtgtt gtctttttgg
     2881 tagtttcagg aacttctttt gaggattctg cctgggcttg atcaggttca tgaataagag
     2941 cgggatgatc gagtgaatcg agcggtattt cattttgagc ttcagtagtt acaggacttt
     3001 cattcccgct tcttaatgcc caatatccta taattgagac tccggtaatt attgctatgg
     3061 atgtaattaa tttgaaaata gtttgatcaa agatactggt tgtaatagag attgattcca
     3121 gttgtgcttt aagttcagca acccgtactt cctttaatgc ttcatttata tcctcctgaa
     3181 attcaaattc ctttttaagc tccggatcct ggcttactgc ctcttcaaca tccctcattt
     3241 caacctcagt cagggttctt tcaaagtagg cttctatttt ttccttaatg tccattaact
     3301 aatcaatcta aaaaatcttt ttctgaatac tgtgatttaa ccaactcatc aagtttcttt
     3361 ttgcatttgt attttttcgt ttttgctgta tccgtgttgg caaaacccag ttttgaagct
     3421 atttcagtca tggacatttt ttcataataa taataggtaa gtactttacg gcaggtatca
     3481 ccgagttggt tgatgcaatt tttgatgatc ctgattctct cagccctgtc catatccata
     3541 tccatgtatt caggagtatc tttttcttca tttgaaagtc gcttctttct gtcaagttct
     3601 tttctccaaa ggtttaaaca aatactataa atatatgtac tgattttgga agtaagggtg
     3661 aggttgccgc tggttgcttt ctgccaaaat actattaacg catcctgata aatatccttc
     3721 gcctcttgtt cgctaccgct attagtgatg actaacttgg ccatcatccg atagtatttt
     3781 ttgtaaagtt cttccagagc tttttcatca cctcggcaga ttctttcaaa aatctccttt
     3841 tccttcattc agctctatgt tttatactat tgtcaaaaga ttggtaaccc agcaaaatta
     3901 tatattttga gctgataaag caggatagag acaaattaat acccgaatac ttgtccgatt
     3961 cctggaattg cttttgaata gacttctgcc ggcgcttagt ttgatttttt aaaggatatt
     4021 acttcttctt caggcatatt aggatttaag gttgaaacct gctcaatctt atcagattta
     4081 acctcagctt tatcgttgtg cttcttaacc gaaatatgcg gtgcgataat aagtgcgaca
     4141 atagaagtta atttaattaa gatgttcatt gaaggtcctg aagtatcctt aaatgggtca
     4201 ccaacggtgt cgccggtaat ggatgccttg tgagcttcag atcctttgta ttccatcttt
     4261 ccatttattt caacgccctt ttcaaatgat ttctttgcgt tatcccatgc accacctgca
     4321 ttattctgaa acattcccat caggacaccg gatacggtga ttcctgccag cagacctcca
     4381 agcacttccg ggccgaatgc aaatccaact aaggtaggta ctacaagtgc cagagacccg
     4441 ggcaagatca tttcccttat gctggctttt gtggatatgt cgacgcattt tccgtactcc
     4501 ggttccgcgg ttccttccat aatacctgga atttctttga attgcctgcg aacttcttct
     4561 accattgcca ttgctgctct tccaacggcc gctattgcca gggaagaaaa cagaaaggga
     4621 atcatagctc cgacaaagag gcccgccagc acttcagctt tgtaaatgtc aatagaagaa
     4681 atcccggcca ctccgcaaaa agctgcaaag agcgcaagag cagtaagcgc cgcggaagct
     4741 atcgcaaacc cttttccagt ggcagctgta gtatttccaa ctgcgtccag gatatcggtt
     4801 cggccacgca cctcttcagg taaaccactc atttccgcga tgccgcctgc attatctgca
     4861 ataggaccaa aagcgtctat cgccaattgc attgccgtag tagccatcat cccggcagca
     4921 ctaattgcaa caccatatag accggcaaat tgatatgcag tataaatacc tgttgcaaga
     4981 acaagtatag ggaggaaagt actttgcatt cctactgcaa gtccggcaat gatattagtt
     5041 gcatgtccgg tggaagattg tctaactatt gagttaaccg gttttttgcc aatcgccgta
     5101 taatattcgg taataatact cattaatgcg cctacaataa ggccgacaaa tatggcccag
     5161 aaggtatcca taggggtaaa aactactccg cgagttgaaa gagtttcggg gagcatccag
     5221 tttacaagga agaaagatgc aataacggtg agtatgatgg aaacccaatt tcccatatta
     5281 agagcatttt gaacatttcc accttccttc actcttacga gagttgcacc aataatggag
     5341 aaaataaggc cgaggcctgc aataaccatt ggtagcagga caggagcaat tcctccgaag
     5401 ttatcattgg aaataatttc gcggccaagt accatagtgg ccagaatggt ggccacataa
     5461 gaaccaaaaa ggtcagcacc catacctgcc acgtcaccta cgttgtcccc aacattatcg
     5521 gcaattgtcg ccggatttct tggatcatct tcaggaattc cagcttcaac ctttcccaca
     5581 aggtcagcgc ccacgtcagc ggctttggta taaataccac cacccaccct ggcaaataga
     5641 gcaatgcttt cagcgcccag tgaaaagccg gctaatacct cgagtgcttt ttccatttcc
     5701 agcccattca cccctgcatt gaagttcagg acataaacat gataaaaaat gataaagagc
     5761 gatccgatgc ctattacagc caggccagct acaccaagtc ccataactgt acctccggta
     5821 aatgaaacat tcaaggccct ggacaagctg gtgcgggcgg cctgcgttgt tctaacgttg
     5881 gctttcgttg ccactttcat accaatgtat cctgcagttg cagagagaaa tgctccaatg
     5941 agaaatgaaa tggcaattac aggggaggag ttttctacta ctgttccgga ataagccagc
     6001 aaaagaccta cgataattgc aaagtaggat aaaaccttcc attcggcctt gaggaatgcc
     6061 attgcgccat cagcaatatg tttcgccaga tcctgcatct tctggtcacc ggcatcctgc
     6121 tttgaaaccc aggccgattt gcctatcata aataatattc caattaatcc aagagctgga
     6181 attacatata taaagttttc catttgtaat cagggcttaa aaaacttgcg caaagataga
     6241 tgatgatgtt ttatttaaaa aacatccgcg taaaaagtaa aatgctcgct cattcacgaa
     6301 tcttatccag gagtttattt agaaccttaa tttcttttga atccagagtc ttgagaattt
     6361 catcaaattc ccgcacttta tcatcagtct tcttaagcag atcaagaccc tttttagaaa
     6421 ttcgtaatga agcagaccgt ttatccacag aattaatttc cctggttatt aattgcttgt
     6481 ttctgagcct ttccacaatt cttgaggcat cagacatttt gtcgagcatt cgatccttta
     6541 tcaggttgat agtagccggg ttcggatact gtcctcttaa aatccgtaat acattatact
     6601 gttgccgggt aatctgatgg tctttaaaaa aataattaat cttgttgata agataattgt
     6661 gtgtataaag gatattaatc agagcttttt gataatcgct ggtgaatgat ctttgtttta
     6721 gatctttctc taattgcatc ttgaaaaaac ctgcttggaa ttcttttgca caaagataga
     6781 ggttctccta cttactattt atataattaa ggaattccct tttagtattt tcttcgctaa
     6841 attttccttt gaagattgcc gttcctgttt tgctatttgt atcaccaact cccctcgagg
     6901 atacacaaag gtgattagca tcgattacca ctccaacatg aggcgtctta agaacttgca
     6961 taagttcgtc cgcaatttga actgttaatc tttcctgaac ctggggtctt tttgcaaaat
     7021 actgaacaat cctgttcatt ttagagagac caataacctt tcccgttgaa atataagcta
     7081 catgagcttt cccaaaaatg ggaacaaaat ggtgttcaca atgtgagtag aaagtaatat
     7141 ctttctcaac aaccatttca ttgtaaccaa atttattctc aaaaagcctg gcttttggtt
     7201 tattctcggg attcaatcct gaaaatactt ctttgacata cattttagca acgcgattag
     7261 gagttccctt caaggagtca tctgtcaggt ctaatccaag tgtttccatg atctcatgga
     7321 aatgatgggc tatcttttta atcttggcat catcatcaat tgaaaaagca tcatctctca
     7381 taggtgtttc aatggaggtg gcaacatggt catccccata tctttcaata tctgatgccg
     7441 tatatgtact gggatttaaa ataatttctt ttggtctctt catctttgtt tatttaagcg
     7501 ggatactcca cataatttct ttcagtttcg tagagtaaaa cttttaattc aaatttcttg
     7561 ttgattttag ttctcaataa attccagatt gttattgcga tattctccgc cgtcggattg
     7621 caatttttga attcgggaac gtctaaattt agattcttgt ggtcaaatct gctaattatt
     7681 tccgctttta caatatcgct taatgctttc aggtcaaaga catacccggt ttcagcatcg
     7741 ggctcaccgg taagccggac caccaattca taattatgtc cgtgccagtt tggattatta
     7801 cacttgccaa atacctcctg atttcttgtt tctgaccagg aaggattgtg aagcctgtgg
     7861 gccgcattaa aatgttcttt tctgtaaact gatactttca ttgaaatagt ttaacacctc
     7921 agaaatggga tactgttctt gaaacgcaaa tttcctgcta ataattcaaa aagttgcctt
     7981 taaatcttca attcctcctt tcgcgcgctt ccagtcaagt taaataattt tccagccaag
     8041 ctaagtaatt taatatggtg cgctaaatta tgacaatacg aagtgtttat gccattttca
     8101 caggcttgat ttgtgtctta caatagtttc aaatagttat atttggaata atcaatttat
     8161 ccaaacaaaa taattaaatc atgagcaacc gtcgcaaatt cattgaaaga gcggtgggat
     8221 taggattatc gcttcccctc tttcctgcca tagccaaagc ttctgctcaa tattcaaatc
     8281 ctgttaaagc tgccaaactg ccactaatct tatgcagcag gggagaatac tgggggagaa
     8341 aagtgcttga tccggcctgg gaaaagctta aaacgggaag tattctggat gctatcgagg
     8401 ttggagccaa tgttgtagaa cttgaccctg aagataattc agtaggatac ggtggcttgc
     8461 caaatgaaga aggaatcgtt caactggacg cttcaataat gtatggaccc acacataatt
     8521 gtggctctgt aatggcattg gaaggagtaa aaaccccttg ctctgttgcg cgattagtaa
     8581 tggaacgaac tgaccatatg gtattggccg gggaaggtgc ccagcgattc gccagggcgc
     8641 acgggtttaa gattgaagaa cttctaacgg atagatccag aagaatttgg ttgcgttgga
     8701 aagaaaatat cagtgaccgg gatgactggc ttccacctgc cgatggaaac tatggactgg
     8761 agcgaagcac cggaacaatt aatgtattgg gaatcgatac tgaaggaaat gtggcaggta
     8821 taactactac cagtggatta ttcggtaaga ttcccggaag ggtaggagac agtcctatta
     8881 ttggggccgg gctttatgtg gataattctg ttggtgccgc gggtgcaaca ggtcgcggag
     8941 aagaagttat cagaacctgc ggaagttttt acgttgtaca gctaatggag cagggaaaat
     9001 cacctcagga agcctgtgaa ttggcctgca agaagattgt tgatattaat ggcggcccgt
     9061 caaaagcgga cgtggatgac atgttcgtgg ctattaatgt gaatggtgaa gttggggttg
     9121 cttctataaa aggaagaaag gataatctgc ctcaggctgc ttattatagt gctgaagggt
     9181 ttaaagtagt gcgtggaaca tatctgatgg aaagagatta gaatcttccc ttatcttcgg
     9241 gatacatgga aggtacggga tcacttgtaa aaaaatcttt tgagtttata tccatcatgg
     9301 aaagacatcc attccagccg gcacctgtat ccataaacca aatttcacaa gctttaattg
     9361 ggtggttgta gccataagca tgaatgggag tgtggcccag ataaatttca tcaaatgagg
     9421 tgagttgctt ttctgcttta gctgtcctaa gttcaagggc actggtggca agctttctat
     9481 cccacaaaaa ggtatttgaa gactggtcat tcaagtcgat ttcgggttga actccggcat
     9541 gaacaaacaa tgtattgtca attaaatggt atagactgga gttttccagg aatgctttgt
     9601 gtgaatcatt cggcagtccg ttataactcc tcacagtagc atctccaccc tggacagacc
     9661 aaatttcccc gggaaattcc cctctcatcc attcaagagc ccaataatca tgattaccca
     9721 tgatatagat caactgtcta atgctcatca gtgtattgat actttctttt gtttcaggcc
     9781 agccgtcaca aacatcacca aggcaaatca gattatcttc atctttgttg aatcccgatc
     9841 tttccaggca ttgcaataag gctttatgtg caccatgaat atctccgata acgaatgttt
     9901 ttgccattag caaaataatt agtttgggta aattttttca atgagcagga gtttctaaat
     9961 actaatcatc atataagtct aaggaagaat cttcaggtag attgggaatc tgaaatcgtg
    10021 gagacacatt aaaagacaag gaaagccgaa tctaactgaa gaattaaatg aatttctgaa
    10081 tcagttttca ttctaattca ttcaaaaaca gaaatgttca ttgtctgggt agttgcttca
    10141 gattttttct gagcatccaa ccaactctgt aagtctgact gccttcatcc atcaggacga
    10201 gccaccagat tctgcgattg gaatctgcac tggatgccac tgcaaaacct ctgtcaccgc
    10261 ccttgtacat tttgataaca ttagtagctt ctgaagtcgc agcccagcgc aattccggaa
    10321 tttgcagaat ttcaaaaccc accgggggaa cattttcatt aatcaggacc agattgggat
    10381 ggaagagaag agtattttct acttcatagg taagctttcc atttcgaaaa accgggatat
    10441 aatattttac ctcattttca gaattatttt tttttatcag tgtttgaaaa ttaattggat
    10501 tccacggttt tgcccggcct atattaataa ttgattccga tacttcgtac tccatttcca
    10561 attcataatc cgtattaatc atgaagccac ttttttgtcc cggtaaccct ttgtaaaaaa
    10621 catctaaata tccatcgcta ttgatatcca gtatgttata ctgctttatt tcaagtacta
    10681 cttttataga atccaaattt ttgattgaat cagtcaggaa ttcaattttt ctgtgaagcg
    10741 aatcaatggc aacccactgg ttcaaattga aatccttact ggccagatat atatttatct
    10801 gactcaagga atttgtaaaa gcaaatatgc ccagaatggc tgtcaatgtt aagtaatgca
    10861 ttttcataat tggtggatgt ttggaaacgg aactttgaat ataggcagaa taaccatttc
    10921 aaaccagttc ggaattccaa aaaaataacc tgtatggagt tcatcattta tggcaccatc
    10981 cttgataact attatactta attaagtaga agttgaaaat attaatgagt aaactatggt
    11041 gagttttatt gtatttgcgg gtatcacagt tgttttaggt gcagcgacgg gtttattttt
    11101 aactttggtg atgccaaaat tctggataaa gctaaagaaa taaaccgggg atttatccct
    11161 ttaaaaaatg aaaagcctca gaatattttc tggggctttt gttaaagagg tcagtgaatt
    11221 aacaatgctc attgaaaact cccatgaggt gctcggcaat tagttctgca ggcctgcctt
    11281 ctatcatgtg tctttcaaca aatgaaacta actctccatc cttaaataga gcaattgatg
    11341 ggctggatgg tgggtaggga agtgtatatt cccttgcctt ttccgttgcc tcaaaatcaa
    11401 ctcccgcaaa taccgttagc aaattcgctg gctttttagc tgagtgcgac aacgccattt
    11461 taattgccgg ccgggcagct ccggcagcac atccgcaaac ggagttaata accatcaagg
    11521 ttgttccttt gcgatcagcg aggtggctct caacttccgc tgccgtttta agttcagtaa
    11581 atccgactcc gactaaatcg gatcgtattg gggtcactaa ttcttctgga tacattctat
    11641 ttcaattttt aatttttaaa gaaaacctaa ttctaattta gctgactctg acatcatttc
    11701 catagtataa ggtggatcaa aagtcagttc aacagttaca tcgttcactt gttcaatttc
    11761 cttaaccttc atctctacct ctccgggaat cgattcagca ctcggacatg atggtgaagt
    11821 cagtgtcatt aaaatataca cattattcac ggggtaaata tttatttcat aaatcaaccc
    11881 aagctcgtag acgtccacag gaatttccgg atcgaaaaca gttttgattg cttcgataat
    11941 cttttccttt aattccggag cattctcaat ctggttatct gtatttatta tgtcttttct
    12001 gtcttccatt gattttggct aggcatttat cttgctctta aatgcgagcg catacatttt
    12061 catctgtttt atcattgaca aaaatccgtt cgatctctgg gttccaataa atcgattcat
    12121 gccaattttg tccataaaaa acaattcact ctcaagaatt tcatccggtg ttcttccatc
    12181 gtaaattcta atgagaaaat aaattaatcc cttggttatt gcagcattac tatccgcatg
    12241 aaaattgatt ttgtcatcct tgagttcagg gctaagccaa actttggact gacacccttt
    12301 caccaaatta gattctatct tcaaactttc atccattgcc ggaagtttct cgccagattc
    12361 cataacaaac aaggttgcca tttccatatc tgctccaatc atggaaaact ctaaaattat
    12421 ttgttcctga acttctgcaa tattcatttc ttcagcttct ttataatttc cctgatcgca
    12481 tcaaacataa tttgaatttc ttctctggta ttataaacac caaaacttgc cctaacagtt
    12541 ccatctattt taaaggcatc cattaagggc tgtgcgcaat gatgcccggt gcggacggct
    12601 attccctgcg catcaagcat tgttcccagg tccagcggat gaacaccatt aatcaaaaaa
    12661 gaaaatacgc ttactttatg tgcagcttca ccgtaaatta caagatcttc aatttccttt
    12721 gatttctcgt gagcaaattg gatcaattct tcttcatgag ctgcaatgga atttctgccg
    12781 tgttcaagga taaaatctat ggattcatcg aaagcgataa catcaccaat attaggagtc
    12841 ccggcttcga acttgtatgg gatatcatta taggttgttt ttttaaaagt tacttgccgg
    12901 atcatttcgc ctccgccctg gtaaggcggc atattttcca ggagaacgcg ttttccatag
    12961 aggacaccaa ctccggtagg gccgtacatt ttgtgagcag agaaagcaaa gaagtcgcaa
    13021 tccaaatcct gaacgtcaat tgacaggtga ggagctgcct gtgccccgtc aatcatcacc
    13081 aatgcaccgg cactatgggc aatttcaatt acttctttta ccggattaac ggttccaagc
    13141 gcattggagg caaacgccag agatactatt cttgttttgt cagaaataag tttcttaaag
    13201 tcttcaatat ctatttcacc tttcccactt accggaatta tttttaatac cgagcctttc
    13261 tcttcagcaa tcatctgcca gggaactata tttgaatgat gctccattcc ggtgatgatg
    13321 atctcatcgc ctttcttaaa attctttctg ccataggttt gggcaacaag atttatccct
    13381 tcagttgttc ccttagtaaa gattacttct tcaatttcag gagcattaat aaattcagct
    13441 gccttctttc tacttaactc aaaagtgctt gtagctttct cggctaaagt gtgcaatccc
    13501 ctgtgaatat tggaattgta ctcccggtag tagctctcaa gggcatcaag aacgcgctga
    13561 ggtttttgag tagtggctgc attatcgaaa taaaccaatt gccgcccatt aacagtttgg
    13621 tgaagaatgg gaaactcgga gcgcagttgc ctcatatcaa gcttgcggat aatattttcg
    13681 ccgacggaat tcactggttt tctcctccaa atcttcttat tatttcctta tccaggtaat
    13741 caaccaccgc tttaacttcg attctgctta aaatatcatg ctgaaatgct tttagtacca
    13801 ttttcaaagc catatctttt gaaattcctc gtgattgcaa ataaaatatt tgctcttcat
    13861 ccagttgtcc ggtggtacaa ccatgggagc atttcacgtc gtctgcccaa atttcaagct
    13921 ggggtttagt attgacagcg gcatcattgg agagcagaat gtttttgtta gattgaaatg
    13981 cattggtttt ctgtgccgaa ggccggacgt agatcttacc attgaagaca ccggttgatt
    14041 tttcatccag aatcccttta tacagttcgt tggaaacgga atttggtttt ttatggtcaa
    14101 caacggtatg gttatcaaca tgctggttat cggttaactg gtacaaacca taaaggtgag
    14161 attcaatatt ttcatcatcg agaataacat tcaggttatt tctaaccatt tttcctccga
    14221 gactgacgca aatggagtta aaattactat cccttgattg ctgaacgtat atgttggtat
    14281 gcgtgatggc ggcgttactt tcattttgaa tttgagttaa ccttgaatga gaggctttgc
    14341 cggagaaaac ttcaaccacc gcatttgtaa aagaagtgta gtctcccttt gagaaatatc
    14401 caacataaaa cgatgatttc gattgctcgc ccaaatcaat caatattcgt ggcatagaaa
    14461 tactattttc ctcctgaaca tctgagatgt ggataatgaa aataggttta tttacctggg
    14521 atgactctgc aattttgatg gacaggcaat ggtcaaagtg aagcgcgtta agcgcgacaa
    14581 aaccattatt ctcaatttta gcaatcgttc cgatctgagc attgtcaggc tggtcgttta
    14641 cctgcaattc tgcaggatag tttgataatt cttttttcca tttaccattt accaatacca
    14701 ggaagtagca gtcgaaatca ggaataagaa actccgaaac tgctgacaat tctgactttg
    14761 atgttggagt aagagtatgg ccaaaggttt tctcaattaa tgtggaaatg gaggtgtatt
    14821 tatactcctc atttttcttg tcaggtaacc ctttggctgc caggtaatca tttgccatga
    14881 ctctgatttc cttgaagtca cttttggaaa aaatattatt tgagtttgcc aattccatta
    14941 gacagcctcc attttaatcc agtcataacc ttttttctca agttgaaaag caagatcttt
    15001 cccgccagat ttaactattt tgccatcgta taaaacatgc acgaaatccg gaattatgta
    15061 atcaagcaat cgctggtagt gtgttacaac aatagtggcc ttctgagggg agtggagttt
    15121 gtttacacca ttggccacaa tttttaatgc gtcaatatca agtcccgaat cagtttcatc
    15181 caggatagca agctttggtt caagcaccgc catttgaaag atttcattac gtttcttttc
    15241 accgcctgaa aacccttgat taagtgatct gcttaacagg gattggtcta tatcaacaag
    15301 tttcattttt tctttcacta aagtgaggaa tgaaaccgca tccataggtt cctggccgcg
    15361 gtgctttctt atctgattga gcgctgtttt caggaaatta gttgagctga cacctggtaa
    15421 ctcaaccggg tattgaaacg cgagaaaaag acctgccctg gccctgtctt caggagacat
    15481 ttccatcaga tcttcaccca aaaaggtaat gctgccctca tcaatgtcat aatcttctct
    15541 acccgcgaga acagccgcga gagtgctttt acctgatcca ttagggccca taattgcatg
    15601 tatttccccg gatttaactt ccaggtttat gcctttaagt atttttatac cattaactga
    15661 tgcgtgcaaa ttctttatcg ctaacataga ttatctgatt aaaagtagtt ggaaatcacc
    15721 cgacacttcc ttcaagggtc agtgcaagca atttctgtgc ttccacggca aactccatag
    15781 gcaattgatt tagcacttcc ttcgcataac cgttaacgat gagtgcaacg gcgttctctg
    15841 tgtcaatgcc tctttggttg cagtaaaaga tttggtcttc tccaattttc gatgttgtcg
    15901 cttcgtgttc aaccttagct gaactatttc caatctcaat atatgggaag gtatgcgctc
    15961 cacatttgtc acccatcaac aaggagtcac attgagaaaa atttctggca ttcacagccc
    16021 gtttcataat tttcaccagg ccccggtaac tattttggga atagcccgct gaaactcctt
    16081 tagaaacaat tctgcttctg gtgtttctac ctatatggat cattttcgtt ccggtatccg
    16141 cctgctgccg gttattggtt agagctacag aatagaattc acctactgaa ttatctccct
    16201 taaggataca acttggatat ttccaggtga tcgctgaacc ggtttcaact tgcgtccagc
    16261 taatttttga attctcgcct gagcagattc cccgttttgt aacaaaattg aaaataccgc
    16321 ccttgccatc tttatctccc ggataccaat tctgtacggt agagtattta acttcggcat
    16381 cttttgccgc aaaaatttca actaccgctg catgcaactg attttcatcc cgcatcgggg
    16441 ctgtacatcc ttcaaggtag ctaacgtaag acccttcttc agcaatgatt aaggttcttt
    16501 caaactgacc tgtgttagca gcatttattc tgaaatatgt agaaagctcc attgggcacc
    16561 tgactccttt aggaatatag caaaatgaac cgtcggaaaa aacggcagaa ttcagtgctg
    16621 caaaatagtt gtcggagggt ggaactacgc ttccgatata tttctgaaca agttccggat
    16681 gtttttttac tgcttcactg aagctgcaaa agataatgcc caaatccata agcttctctt
    16741 taaaagttgt tgcaacggaa acgctgtcta aaactgcatc tactgcaacg cctgttagtc
    16801 tcttttgttc ttccagggaa atacccagtt tttcgaatgt tcttcgaagc tcaggatcca
    16861 gttcatccag gcttttcggt tttacttttt gctttggggc tgagtagtaa atgatgtcct
    16921 ggtaattgat tacaggaaat ttaacattag cccattttgg cacctccatc ttaagccaat
    16981 ttcgatatgc cttcaggcgc cactcagtta accattgcgg ctcttctttt ttcgtactga
    17041 taaacctgac aatatcctca ttcagacctt tcggtgctga atcattttcc agatcaatat
    17101 tccaaccatg ttcatattct ttggaagtaa attcttcgag gatatgggaa tctttactca
    17161 tatctacaaa ggtactataa attcttattt ataattgttc caaataagtt ttattgaggc
    17221 taaaatgccc ttaaatttga taaatttgaa tacttagatc actaaggagc aagttgtggg
    17281 caaggcctac cggcaactta actgataaaa aaaaccgtaa attgaggaat gattcaaaga
    17341 atatatacac atcggtcatc ggcggctact ttgatgaaga atttgaattc tacacaaaac
    17401 tgttctttga gaaagttgag caaggaattt ttaaaatgat tctatcggac atattagcaa
    17461 cagaactcaa tggagcacct gaagatgtat taaaatttta ccaatcaatt ccaaagaatc
    17521 aaatcgaata tatagaccag actgaggact ctattcgaga agggaagaat acctaaaagg
    17581 gagttgttgg aaaaacaagc aggacggact gccgacatat tgcgttggcg acacttgtta
    17641 acgctgatat tttgagttag ttggaacttt aaacatatag ttaacattac aagaattaga
    17701 ggttacaact cggttaatta ttaaaaacgg acaccgaatt ctagaaataa ggacacccag
    17761 agaaatatta gaattatgaa aacagaaaca agaagacttt tgacgctgtt gcgttcatga
    17821 gaaagcggag ggatgaaatt tcaaaagaga ttgaagggat gacaccacaa caggagattg
    17881 agtattccaa aaaaaatgca attaagctga aaaataaaaa gtgacgcaca acggcccagc
    17941 ccacaacaaa ggagtataat caatgagcct gttttctgca atgtatttac attcttttac
    18001 taacttagtt tctgtaatgg ctgacagtga tgcgtaagtc gggcttggca ttcgttactc
    18061 atttagcaag ccctcctatc aggccactac taatcacggg tcggtcagaa gacatgcttc
    18121 atgcaaaatg tttgattgaa atctttcagt atatttaaga gtaaattttt ctgacttaac
    18181 ttaactaaag cgaaatggca gattttccct ggtataagca ttatccaaaa ggagttgcac
    18241 aagaaatcaa tcctgatcaa tactccagtt tggtggagat gttagacgaa gccagcaaaa
    18301 aattcagcag ttccccggcc tttgttaata tggataagag aatttcctat aaagaaatga
    18361 aggagaaagc aactgaattg gctgcctttt ttcaatccat tggtttgaaa aaaggagaca
    18421 gaattgttgt acaaatgccc aatctgcttc aatacccggt gataatttac ggagccttaa
    18481 aagcaggtct cattgttgta aataccaatc cgctttacac acccagggag atggaacatc
    18541 agttcaaaga ttcgggtgca cgggcaatca taatacttgc aaattttgct tacaatcttg
    18601 aaaagattat tcagaaaacg aatatagaaa cagttatcat tacggagata ggggatttgc
    18661 tgggtggctt caaatccatg gtagtgaatt tcgcagtgaa gaagataaag aaaatggttc
    18721 ctgattacca tttaccggga gcaatcaagt tcaatgttgc tctaaaatca gggaggagta
    18781 aaaagtttac tccggttgag cttattggaa aggatgtcgc atttctgcaa tatacgggag
    18841 gaactaccgg agtttctaag ggtgccacat taacgcatcg aaatattgtt tccaacattt
    18901 tacaatcaaa ggaatggttt gtgggcctta aggagaaaga ggaggttgtt ataactgcct
    18961 tgcctcttta ccacatattc gcactcaccg cgaacttctt taacatgtat atgataggta
    19021 cacttgacgt tctgattacc aacccaagag acatgaaggc tttcatcaaa gagctcaaga
    19081 agtatccgtt tacagtcttt ttaggagtta acacattatt taatggatta ctacatcaac
    19141 ctgatataag tgacgtgaat ttcagtcact taaaaatcgc cgttgccgga ggaatggctc
    19201 tgcagaaacc tgtggcaacg ctgtggaaag acaaaaccgg gaattcaata gctgagggat
    19261 atggcttaag cgaaacttca ccggttttaa gctgcaatcc ccttaatggc actgaaagaa
    19321 ttggaactat tggtatccct tatccaagta cggaaatgaa gataatggat gaaaatggaa
    19381 atgaaatgcc attgggtgaa cccggtgaaa tttgcgctcg cggacctcag gtaatgcccg
    19441 gttattggaa tcgtgaagat gaaacagcca aatgcttctt tggtgaatgg tttaaaaccg
    19501 gagatattgg cgtaatggac gaagatggat tctttagcat tgtagatcgt aagaaggata
    19561 tgatcctggt atccggattt aatgtatatc ctaatgagat tgaagatgta atttcaggtc
    19621 atccgaaagt tcttgaagtc gctgccattg gtattcctga tgtcaattct aatgaagcag
    19681 taaaagtttt catcgtaaaa aaagatgaat ccctaaccga acaagaggta catgattact
    19741 gtgtggaaaa tcttaccgga tataaaagac ccaaggcgat tgaattcatg aaggaattac
    19801 caaaatcaaa tgtgggcaaa attctccgca agaatctaag agaagcagtt tcttagtgga
    19861 ttctgtagcc tattcctgag ttcagaaaaa tatccgtggc ataagtattt aaccctaagc
    19921 caattgcaag gtcaatggtg aagttatctg ttaaggtaaa gccgacaccc ccatcaagtc
    19981 caacagcatt agggccgcca tttataacat tgctgaaaaa ctctgtaaag gcaccaagcc
    20041 ggccaaaaga agcactaagc atgattgaat aaaatccgtt tgtattgcct gaaaaatccg
    20101 aatctgtgcc aatggagtag gccaggctta agttatccga caacggattt tcgagcaaaa
    20161 gtcgggtgtt tagagcaccg ttattatctt ttgcaaaacc tgttccggtt cctggcaagg
    20221 ttaaataggc agtggccgag attctgggga ttaatccttt tggttccagt attttcaatt
    20281 ttggggcaat aaaaattcct ccaagttctg tatttgttgt tagtatggag atgttttctt
    20341 gtttttgata aagaagcgcg gtgccggcac gtaactcgag ccatggtaac aagccatatt
    20401 taagcatcag attaggaata ttcatcgaag tagtggcgtt gcccagaacc tcgtcgttgg
    20461 accaaaaata tccggattca atctggaaga catttttggg taataatgca actgcatctg
    20521 tctgactggg ccggtcggtg aaaatgctcc caatatcctg ggcttgtgta aagcctgcca
    20581 tgcttatcat gataagaact aagaattttt tcttcatgtg aggtgttttt ggaataggta
    20641 ttataattaa aaatcttaag caaaatatac caaattcact aaagttaata cgtcaaccgt
    20701 ttggatataa tatgcaactg gcggataatt gccgtctgat ttgataaaat tatgaaaagg
    20761 aaaataattg aaaccttatt aattatttca atacttcccg gctgttccgg cagcagcaat
    20821 gaagaacagc aaagcgacga acttgcagca gttgatactt tagtttatga gggtgaaaag
    20881 aatctgacca acgtaataca attgacattt ggggctgata atgctgaggc atattggagc
    20941 ttttccggag agatgattac atttcagtct aataattctg aatggggagt taattgtgat
    21001 caaatttttt ttactgaagt ttcagacgct aacccggtta cccaaaaacc tcaaatgata
    21061 agcaatggtc tgggaaggac aacttgccca tactttatgc cgggagattc aactattatt
    21121 tttgcatcca ctcatcctgg aggaaatgat tgtccgcctg aaccagaccg gagttcagga
    21181 aaatatgttt ggccggttta ttccagttat gatttgttta ttgccgatct gaaaggaaac
    21241 attattaccc ggttaaccga tgaacctggc tatgatgccg aagcaaccct ttccccaaaa
    21301 ggtgacaaaa ttgttttcac cagcgatcgc tctggtgatc tggagttata tatcattaat
    21361 actgatggaa gagggataac ccagattacc aatgaacttg gttatgatgg tggggcattt
    21421 ttttctcctg acggcactaa gcttgtattt cgggcctcaa gaccgaagac tgaagaagaa
    21481 attagtgaat ataagaattt gctttcccag ggtctggttg aaccaaccat gatggagtta
    21541 tttgtatgca atatagatgg cagtaacatg cgacaaatta ctaatctggg aaaagccaat
    21601 tgggcaccgt tttatcatcc ttccggaagg aaaataattt tctcgtcgaa tcaccatggg
    21661 tcaattggat ttcagtttaa cctgtttatg attaatgagg atggaagcgg gctggagcag
    21721 ataacttttg atcctgtttt tgattctttc ccaatgtttt ctcccgatgg taagagatta
    21781 atcttcagca gcaatagaaa taataaaggt acaagagata ccaatttatt tattgctgaa
    21841 tgggtagaaa actagaagat gattattcca aagaattttt gaagaacggt tactaccacc
    21901 ataattccca gcagcacggg gtttacccaa atgatcataa aatgcaaaaa ggttttcaga
    21961 attcccgtta cgaaatttga attaccctga gtcatttctt catcaaaact tttcattccc
    22021 cattttttat tgatgaaaat catcattaga aatcctccaa gaggaagacc tacatcacta
    22081 aacacatcaa atacaagatc gaaaaatgat ttatcaactc cttcgtaatg caggaagcgg
    22141 gtaaagaaat caattgcacc gtgagaaagc atactgggga gactgaccag aaaaataact
    22201 gtagcggata gaattaccac gcgcagtctc ggccatttct tttcgtctac cagataagtg
    22261 gttggaattt caagaagaga aattgtagaa gtaagcgcgg caaaacaaag caataaaaag
    22321 aaggctccgc ctacaatttt tccggtaatt aatcccatct gggcaaaaat acccggtaac
    22381 gcaacgaata ctaatccggg tccctgggtt ggagtctgtc cctggctaaa caccagtgga
    22441 aaaatcatta agccggccag aaacgccacc atggtatcgg taaaaaccac cataccggcg
    22501 gaattgccaa tattttcctt tttactgatg taggatccat aggtaatcaa agcacccatt
    22561 cccagggata atgaaaagaa agcctggccg agcgcggaat aaattgttct gacatttatg
    22621 aggctaaaat cggggataag gtagaatcta acaccttcca tggcattttc cagggtgagg
    22681 ctatatgcta ttaacccaat caataataaa aacagaaggg gcatcaagat tttggatgcg
    22741 gtttcaattc cttttttaat tccgccggca actataattg ccgtcagtac catgaatccg
    22801 agggaataga aaaaattatc agaaatatcg gcgatgtaag tgccgaattt tcctggcaaa
    22861 tcagaagtgg agagcagatc gccgaatgag atttgcagaa aatacccaaa ggcccatcca
    22921 gccacaacat tatatgccga caagaacata ataccacaaa ttattcccca gattccaagc
    22981 aatccccagt tttttgttcc aagtgagcga taagcccggt atggatttgc ctgcgcggaa
    23041 cggcccaatg agatttcacc aaccatcacc gggtatccga taatgatgat gcaaataaga
    23101 tacaccacga ggaaggcggc acctccgtgc tggccggttt catagggaaa tctccagata
    23161 tttcccaggc caacagctga tcccgcagcg gctgcaacaa acccaattct actcgaaaaa
    23221 ccaactctat tcataatttc agtaggtaaa gggttcgaaa atatggacaa atactgattt
    23281 ttgaaaatag agacccttaa aattaggtgt gatcagatac ttcgggtttt tcagacgtcg
    23341 gagcagagcc attatcagga ttagcttttg caaatagctt acccacagta agtccctgga
    23401 caaggattga aaagagtacg acactgtagg tcatggaaat tatcaaatct ataatccggg
    23461 ggtttacact gaattctttt gcagtatcat acagggaaag ggcgagtgca accgatatac
    23521 caccccttag cccggaccat gtgagaattc gtattgtgta ttttggaatt tcgatcctgg
    23581 ttctaataat gcttattgga atactaacac caatgaatct tgccagtaga acaattataa
    23641 tggcaattcc ggcggcaaca aaaaatccca attcccaatt gattactatt acttgtaatc
    23701 caatcaggat gaaaaggata gcattcattg cttcatccat taatgaccag aatttataaa
    23761 cgtattctcc agcaattgga tctgtttctt cccctcttcc tttttcacca acaaatattc
    23821 ccattacaac tgttgaaagg ggcatggata cgtgaagcat ttccgctaaa cttgctccac
    23881 ccaaaaccat ggacagggta acaagaactt caatttctgt gtgttcatta tcaatgataa
    23941 cgagcaacca tcgtcccaca tagccgatgg cggcgcctaa taaaattcca ccaactacct
    24001 caactccaaa taattctgcg acatgcattg cactaatttg agcttcggcc aaacctcctc
    24061 ttggaaaagc aattgaaagt atggtgagga agataacaac gccaatacca tcattaaata
    24121 acgactcccc ggcgatttgg gactccaggt tttttgaaac atgagatttc tttatcatag
    24181 ccagaacggc gatgggatcg gtaggtgaaa taagggcacc aaaaagcaaa caatggatta
    24241 actccaattg tatattcacc aatggaagaa gaaaatacat ggcataccca acaacgaagg
    24301 ttgagatcag tgttccaaac acggccagaa ttccaatcac ccacttctct tctttcaatt
    24361 tcctgagatc catttcaata gctcccgcaa aaagcaaaaa gctcaacatc accttaaaca
    24421 acacttcgga aaaattgtat ttggtcatta aatcctgtgc gagagtattt aatgttttga
    24481 aaacaagttc accgaaaatg atgaacaggg aaagacataa agcaaggatc atgagaccaa
    24541 tagtggacgg aagtttgaga tacttgacgt taaccagggt gaaaaaggcc gccattatga
    24601 tcaagagggt gataaaatct aatacatcca ttgggttagt agtaagttag tttgaaaatt
    24661 gggttcttat tggtagtcaa tttaggaaat tcaaccaata attaaaactt caagacttcc
    24721 ggggatcaag ttctccaaat cgttaattcg agacgaattt ggcacttttc ttgatgattt
    24781 cgatttagaa agcctacctt tgaggcagaa ttgaatcgcg cctgggaaga cggcaaacgg
    24841 gtaaaaatgg gaacctacag gaaccttact actaaagaga aggccttaca gataaatctg
    24901 gattcatcca tttatggttc ctttgcagaa atcggtgcgg gccaggaagt agcggcaaat
    24961 ttctttaaag ccggaagcgc ctcgggtaca attgccaaaa ccatctcagc gtacgacatg
    25021 acattcagtg atgccattta cgggcagtgt gaacgatatg tctgtgaacc caggttaatg
    25081 ggaatgttag gcaaagaatt taacctcctc cggaaaagac tgagcagaaa agcggataag
    25141 aagaaatttt ttgccttcgc ggatacggtt gaaacctccc ccttcggtca catgcgcgat
    25201 ggtcatggct ggattggcct tcgatttcaa acatcaccaa aaagagaacc gaatgaatgc
    25261 attattcatg tgcatatgct tgatgacgat aatgtgttac aacaacaaac tttaggaatt
    25321 gttggagtga atttaattta tggatgctat tttattgatg atccggaagc attaattctt
    25381 tccttaatgg atgacctgac atcaaggaga atcgaaattg atatgttcaa acttgaaggt
    25441 ccggattttc agcatgttga caacagatta atgagtttga aactggttaa aaatggttta
    25501 accaaagcag ctatgtttgg cccggatggc gaagtgttgc aaccctccga agttctttac
    25561 aagaaaaatg tgctggtttt acggggaaga tttcgtccgg taacccacgt aaatattgat
    25621 atgcttcttt ccggaaggag agagttcaaa aaagatcctg atgtagataa gaaagacatt
    25681 cttgtcattt ctgagttaac ccttcaggat ttgagtaatg aaggggagat tgacgatctg
    25741 gattttctaa gccgggtgga tatcatttgt tcactgggac aaaatgtgat gatctccaac
    25801 tattacgagt attacaggct tgttgattac ctttccttat atacacgaaa gaagaagatt
    25861 ggtatcatcc tggggtttaa caatctggcc agagtttttg atgaaactta ttatgagaat
    25921 ctgcgtggcg ggattctcga atcattcgga acgttgtttg gacataacgt caccctgtac
    25981 gtttatccgt gtcgtaatca ggattcaaat gaaatttgca caaccaagag ttttaatgtg
    26041 cccaggcacc ttcggggttt gtttgattac ctgatggata accataaact gcaggatgtt
    26101 gaaacagcaa atcttgaact tttggatatt atctctgaca atgttctgat aaagattcaa
    26161 agaggtatag ctggttggga agaaaccatg ccgactaaaa ttgctcttgc gataaaagaa
    26221 ggtggacttt ttggttacaa agctcctgct gaacaacaga ttacttaagc ctttcaggcc
    26281 aactctctca agtctacagg aactaccctg gaaactcctt gctcaatcat cgtgatacca
    26341 tagataacat cggtatacac catagttcgc ttattgtggg taacaataat aaactgagat
    26401 tcctttgaga attctttgat aatctcattg aacttgtcaa tgttagcatc atccaatggt
    26461 gcatcaacct catcaaaaat acagaatggt gccggcttga gcagatagat tgcaaacagg
    26521 agggaagtgg ctgttaatgt tttttcacca cccgacaatt ggttaatagt gatgggtttt
    26581 ttacccttag gttttgcaat tatttctaca gaagattcca ggggattttc caaatcagaa
    26641 agaattaaat cacaatcatc ctcctcagtg aataatgatc tgaatacttt aacaaaattt
    26701 cctttaattt tctcaaatgc atccagaaaa gtattttttg ccactaagtc aatttcagca
    26761 attgtcgcaa gcagcgattc tttcgcgtta atcaggtctt gtttttgggt ggtgatgaat
    26821 tcatatcgtt tctggatttc ctgatatgct tccatggcca tcgggtttat tggacccatc
    26881 ttttccatgc gctctttggt tctggtaacc ttgcctttca attcgtcaac ctcctcatct
    26941 tccaccttcg gaagttcttg ttgcattata tcgtccaggt tgatattgaa ttcaattgac
    27001 aatctttctt ttactgaatt taattccagt ttggtttcat tcaacttatt ttgaagttca
    27061 agcaagagat catctaccat ttcttttgct ttatgagctt ctctttcttc tttctcttta
    27121 tcatctattt tcccacgctg ttcatagtag tctttctccg cttcatttac cagcctgtct
    27181 ttctcctcct tctctttata caacttcacc agttcatcat cttgtgaagc ggtgttggca
    27241 atcaacacct ggatatcttg ttcattgagt tgcagctctt ctgaattctt atttattctc
    27301 tcggagcttg attcaaggga actctccttg aatttcaatt cctgatcaat gctgttaact
    27361 ttgttttgct gctgatgaaa atgcaggttt tcctcattgt aagcagcgga tttctcattt
    27421 agaatggcat tttgttcttt cagattgatg gtcagcaatt tcaatttcga ttccatctca
    27481 ccgagctcct gaccttcatt ttcagtcttc ggttgcaatt caataatctc ttgtttgagc
    27541 tgattgattt tatcaatcat gtcttcttta cgcgtatctg tgctacttag taattccctg
    27601 gtttgttcat gtttggtttt aagagaaatt aattcttcat tcaaaagatt gatttgattt
    27661 tgcaattctt cgatctcttc cttgtgtgtc gcatttatta aggattcaag ctgagcttct
    27721 gaatcacggc gactttgttc tgcttcgtct aatttagtat taagtgaatt gatttcaatt
    27781 tccagcttct ccaggttctt ttgtcttcca attctttttc cttcaaaaag acccacggag
    27841 ccaccggaga tactaaattt cttacgggta actttaccat ttttagttat aaaaatgcaa
    27901 tcttcatggt tcggtatttc cttgtagtca ccgattacta tgaagacatt atcaaacagg
    27961 aagctaacca gtttcttgta ttttggatcg aactccagta tctcagttgc cgggaaggcg
    28021 ttatcaaata tttgggttga agagggtttg aatttttcaa aaagattaag gataaagaaa
    28081 tttgcctttc cttttgccga atcgctgaga agattaatag ccctgtatgc ttcctccggt
    28141 tgctctacca cataatgatt catccatgac tccagaaaat tttctatggt cactttatat
    28201 tcttctccgc aggaaatgat atcggaaagt aggggtactt tttttcccca gccggcttct
    28261 tttttaagaa actttatggc ttcaggaaaa ccttccagat tttcaatcat ggatttggtc
    28321 agattatact cattttgtct cgcatccagc ttgcggtgta cctgcacaat ctcatcccga
    28381 atcatttcaa ttgttttccc ggaattctct atcctcgatt cgaggttaac ttcatcctct
    28441 tttaattgtt ccttttgggt attgagatct gaaatctgaa atgttaatcc ggcaatcttt
    28501 tgctcaaatt caatcaggtt ttcgcttcgc ttgctggaat cagttgaggt cttctctaat
    28561 tcctgagtaa aggaattgat ctggatttcc cggatttcta ttgacttctt tagttggtaa
    28621 acttcatctt gccggcttcg gagcgtttga ttcagtgaat ttacttcttc ctgcagctgg
    28681 ttggtgagtt ctttgaggtc ctcataatcc ttatgtaaaa cctcaacctg tttccctatt
    28741 tctcccaaaa ccctttgaac ttcttccttt tcctgattta agctctgaat actaaaagcg
    28801 gctctgtctt tacttttgcg atcctgctca atttgttcgc gcagcatatc actcttattg
    28861 ttcagaaacc gaagctgctc attctttatt tgtttctcat tttcgtatgt ccttatttca
    28921 ttcacatgat tattaagcgc cttctgtcga acggagagtt ctttttcctt tgaaatgagg
    28981 tcagtcttaa gcttttcaat ttcggcctca atttctataa tcttattatt taattgaagc
    29041 tttctatcat tctcattttc aatacttttt gatagttctt tgaatgtgtt attctgtttg
    29101 ccaatgctgg tacgagccaa ttctacgcta aattccttgt actcttcctt tagtttataa
    29161 taccgctcag cttgcctggc ttgcttttcg agggatttaa gattcttatc aatctcaaat
    29221 aataaatctt ccaccctttc caggtcagca tcagtatcct gcaattttct cagggtttct
    29281 ttttttcttt gtttgaattt tgagatgccc gcagcctcct caaacaggtt tcttctactg
    29341 ttatccttat cattaaggat gtcatccacc attttcaatt caataattgc ataagaatct
    29401 gagccgattc ccgtatccat aaacaagtta ttaatgtctt tcaacctaca atgaacacca
    29461 ttcataaggt attcactttc tccggttcta aagtatcttc ttgtgattgt tatttgtgaa
    29521 tactctgtgg gtatcaggtt cttagtattg ttgaatgtaa gtgatacttc ggcaagattt
    29581 gttgctttac gcttcttggt tccattgaag atgacattct ccattttatc ggagcggaga
    29641 gattttattt tctgttcacc tagaacccag cgaattgcat caacaatatt ggattttcca
    29701 catccatttg ggcctaccac gcccgttacc ccttcatcaa aattgatagt taccttgtcg
    29761 ccaaaactct taaagccatt tatttcaagt ctggaaagct gcattgattg tttaagtata
    29821 gacccttaaa gtcgacaaag ttaaacttta ttttcataaa tttgatttcg atatggatga
    29881 cagtctacag ataattattt acatcgtatt catcgcgata tatttccttt caagattttt
    29941 gaaaggaaac aagcagaaag catcgcgccc tcgtcccagg caggagtcgg aagaagaagg
    30001 gcaggttgag actaatgaac agcctatgac ctttcaggat atacttcgtg agttgactca
    30061 accgcgtgaa gcgccagctc cacaacccaa aaagagggct ccggttgagc ccgggccgcg
    30121 agattatgaa tttaaaggtg attatccgga tgaagcggaa attcaggaag tatatgaaga
    30181 gtcattgagt agagcgaaga atgtaaaaac tttggatgaa atgattaata tcggggaaga
    30241 atccatcaaa ttcaaagagt accaggaagc tgatcagcaa aatgcccttg caactgaaat
    30301 ccgggaaatg ttaaaagatc cttacgatgc gaaaagggca atcatcttaa aggaaattct
    30361 ggatcgaaaa ttctgaccga aacttactga atatgggtaa aagcgaatga gataatattg
    30421 gctcccattt gcaatgcttt ttgcctgatt tcctcaggat cattataaat aacctggtct
    30481 tcccatccat ttcccaaatc actttcaaag ctgtaaaaac acaccagtct gcctttataa
    30541 atcaggccaa atccctgggg cggttttccg tcatgttcat gaatctttgg cagaccctga
    30601 tcgaagtcaa atttctggtg ataaatcgga tggtcgaaag gtaattctac gaattcaagc
    30661 tccggaaaaa ccttcttcat ttcaagcctg ataaactgat tcaggccata gttgtcatct
    30721 atatggagaa atccgcctgc tatcagatac tttctgaggt tctgggcttc cgggggtgaa
    30781 aacactacat tgccatgtcc cgtcatgtaa atgtagggat acaggaatag ttcggatgaa
    30841 cccacctcca caatatcttc ctcaagcgcc agatgcatgt ttagattttg attgcaaaat
    30901 tttgccagat taggcagggc agttttgttg gcataccagt cacctccgcc gctatacttt
    30961 agtttggcga tcttcaattg ctcctggcca taacaaaaca ttggcaggaa gaaaaacagg
    31021 aatgttaaaa ttttttctat cttgaaggct tgcatcataa aatcataacg tgaattatcg
    31081 ccatgagttt ttcgtttaac gctatttaca ataattattc cgaattccgg gagaattccc
    31141 tgcatggtct gcaagtgaag gcggaagaga tccggccatt attggaaaaa gcgaaaaatg
    31201 tgaacggaat tgaggtttca gaaattggta aatccttttt gggagaaagc attcataaaa
    31261 ttgtaatagg tagcggtagt aaaaagattt tagcctggag tcaaatgcat ggcaatgaag
    31321 caaccgccac aaaggttgtt tttgatttgg ttaatttaat tagcaagagg cctgaattga
    31381 ccggcctgat ttttagcaaa ttgcagctaa ccgtaattcc catgttgaat ccggatggag
    31441 caaaatcatt taccaggatt aatgcccagg gcattgatat caacagggat gcgtatgcaa
    31501 gacttactcc cgaatcaaaa atcttgcata aaatctttgc tgagttgaaa cctgaatttt
    31561 gcctgaatct gcatgatcag cgtccaatct ataatgttgg cgaaagcgct acgccggcca
    31621 ccttgtcctt tttggctccg ccatataaca aagaagtgga ggttaatgaa gagcggctaa
    31681 tcgccataaa aatggtggtt gctttggaga agctgctgac acaagaatta aaaattggca
    31741 tttccagata ttcagatacc tatgaacgca gagcttttgg agataccttt caatctaaaa
    31801 atgtgccaac tgttctgttt gaggcaggcg gttaccgggg tgatcatgac cgggagttag
    31861 tgagaagttt ttattttatg gccttgcttt ccagtttgca ttttttggct gaggattcat
    31921 ttctccatga agacccggat gattacttca agattccggt taacgaggaa ttgctattcg
    31981 attggatcat ccgtcgcgtc aagattacag attccggaaa ggaatggatc gcagatgttg
    32041 gtggtcaaag ggaatacagc gggagtaaat acggtaaggg aattattgag gaggttggtg
    32101 acctgtccca gtactatgga tatacggaaa tagatgggag tggatgcata ttagagaaag
    32161 gcaaggtact ggtctcgcct gatgagaata acaaagatac gcaggaactt gtagccaagg
    32221 gatatacaac aattcaatct gagagctttc cagctggtca ttctttagca ataaacattc
    32281 aaaggtcggc cagggaattc ctaattacgc ccggtcatcc tgccaacctg gttgtattca
    32341 gaaacaagaa aattgttaaa gtcattctta atggatttat ccttgaccca tcccggcgtc
    32401 cctttgaaaa ttattatgga atatccctgg aataaggatt cagtaaatca gtttttcttt
    32461 taactttcgg gcttttatta tcaagatgaa atcaaatgca ataattataa ctgccggaat
    32521 ccttgatgac attcatgcaa agacaacgca tgggctggta aggggaactg acaagttcaa
    32581 cattgttggt gttattgaca gcaaccacgc cggcaaagat gcaggtgaag ttattgatgg
    32641 caaaaaaaga aatatcccgg tattcagaga ctttgaagac ttcaggaata aaagcccaca
    32701 aaaagctgaa ttatgcatta ttggtgttgc caccaaggga ggcagaattc cggcagagat
    32761 gcgtgatgta attaaggaag caataagttc cgggtatggg attgtaaatg ggctgcatga
    32821 atttgtgagc gaaataaagg agttaaaaga attggctggt gagaaaggtg ttgaacttat
    32881 tgatattcga aaacctcgac cccgaaacga attgagattt tggtccggtg aaatttaccg
    32941 gattctatcg cccataatag ccgtcctggg cgtggattgt gccgttggta agcgaaccac
    33001 ttcccggatg ttggttcaat cgatgaaaga tgtgggtata aatgccgaga tgatctatac
    33061 aggacagacc ggctggcttt gcggaataaa gcatggtttt atctttgatg ccacatacaa
    33121 cgattttatc agcggtgaag tggaagcagc tctgctaagt tgttacgatg atcaaaaacc
    33181 tgaagtgatc tttattgaag gtcagtcggg gctaagaaat cccagcggcc cctgcggatc
    33241 tgaatttctt ttatcaggaa atgcaaaagg aagcatactt caggttgtac cccataggaa
    33301 aaatttcaaa ggttttgaaa acctgaattg ttccttgcca tcagcaactg atgaaatcgc
    33361 ccttattaaa atgtataact ccgaggtaat tgggattacc gttaatacag agcatctcac
    33421 taaagaagaa agtttccagg caaaaacaca acttgaaaag gaaacaagat tacccgttgc
    33481 attgccatta tttgagggtg ttgaaccgtt ggttcctgca ctcgtggaat acgttaaaaa
    33541 ttacaaaaga tgaaaattac caggtattct ttctggagac agaatttgga acttacaagg
    33601 ccttatgcta tagctttcaa gactactgat tcagtcgaga atattttttt tgaagtggaa
    33661 ctggcagatg gcaaatccgg tttgggctgc tgcaatgtaa gcgccaaggt agtaggagtt
    33721 acacttgatg attgctggaa attctttaat aatgaagatc tgaattggct ggttggcagt
    33781 gatatcaggg aaataggaag acttcaagct gagttagtga acaaatacca ttctaacccc
    33841 ggagtaagag cggcgatgga catcgcttta tatgatgctt ttggaaaata tcttgatatt
    33901 ccattggtga atttctttgg gagggcaatt gaaagcctgc caacatctgt taccatagga
    33961 ataaagaatg tagaagaaac cgtaaaagag gctgaagaat ataagggtat gggttttaca
    34021 aaccttaagg tgaagctggg ccatagtgtt aatgaagata ttgaaaggct gataaagttg
    34081 aatgaggagt taccggaaat ggtaataaga gtggatgcca atcagggata ttccttaaat
    34141 gatttacaat tcttcctgga aaaaaccagg gctttaaatc ttgagttggt tgaacaaccc
    34201 ttacctgttg atcaggttga tgagcagata aagttgagtc tggaagatat aaatgtatta
    34261 gctgccgatg aatccctggt tgggcctgag gatgttgcaa cccttattgc tccacaaccc
    34321 ttatttggaa tatttaatat caagctgatg aaggcgggcg gaatctacca ggcccggaaa
    34381 ataggggagg ttgccggagt aaacgatatt ccattaatgt ggggctgtaa tgacgaatct
    34441 atagtcagta tcagcgcggc tttagcggca gcgtattctt acccatggac gaaatacatt
    34501 gatttggatg gcagctttga tttagccaaa gatgttgtta aggacgggtt tattctaaag
    34561 gaaggtagaa tgtatcctac tgataagcca ggattgggtt tcaccaggtt atagtagttt
    34621 tctccagtca ccgtattcca tatagataac cgtttcacga gccatacttt ttccgaaaag
    34681 tttttctact acatggaggg ataaatcaat tccggctgtt atgccgcctg aagtcattat
    34741 ttttccttca tctacaaacc ttttaccctc cagaatatta gtttctgaag taatgttttt
    34801 tacatccttg atcacctcat ggtgagtgat tgaattatga ccatctaaca atccggcttt
    34861 agcaaggatt ctcgctcccg agcacaccga catggttatt tcactcccgt ggcttatctc
    34921 attcacccag tcaatgacta actgcttatt tatcaattcc cgggttccat cgccgccggg
    34981 aattatcagg atatcaatat cgggatgatc ctcgaaacta aagtcgggaa gaatattcaa
    35041 tccattaacc gcattaattt cttcacctgt ttcagaaatg gtaaaaacag aaaaaggctc
    35101 gtaattgtta agctgggaag taacggagaa aacttcaaaa ggtccggcaa aatcaagaac
    35161 ctctacgtca tcaaaaagga agatgccgac gctgtacttt ttcatgtcag aattttttga
    35221 agcgactaat aaaccggaaa agggtaaaaa accgcagcat ttcatacgct acggttttta
    35281 tttggttact ttttcttcag cggttccaaa tccgtaaaag gcgagaattc aactttctct
    35341 atttcggatc tgaacacaga ccattctgta tcgtaatagg aattcacttt gttaaccaat
    35401 tccatcagct tggtattggc atgttccatg gtacgttttt cagtttcccc cggagcatca
    35461 agggttgatc taagataacc gcgagccgtg aaaatatacc cggtaactgt tggttcggga
    35521 tttctggtaa ttccctgccg gttatccact tttccaacca tggcatccat aaatccattc
    35581 aggctctccc tggttgtttt gcaaagatcg agttgctgct tgaattttat ggcatcctcg
    35641 cttcgcattc tcctggaata atcatcgacc aatgagattg attctttcaa cctgcttgtt
    35701 acttcactgg ccagggctat ataactatcc aattcttttt gagcatcata aatagctttg
    35761 agttcgcttt ccgaaacctc cagccgcgga tcaaaattta ctttgaccgt tgcggagtct
    35821 ttggtatcac ccaatgtaag tcgaacttta taatttccgg gcaatacatc aactcccgag
    35881 ggtggtccaa agccacctct tctttcaccg cgaccaggaa actccggacc ttcttcacgc
    35941 aaattccaat tatagatata aactccattt tcctccgggg cttttcgctt tatagttctt
    36001 actaattttc cgccttcatt aaagaattcg aaaatcaggg aatccgcttt cttattatca
    36061 gcttttggcg actccggagt caggtttgtt gtggcattat tatttccgcg cctttcgcgt
    36121 gcgctttggt tagaacttgc aaccggtgat tcattgcttc cgggtttatt aaatacatat
    36181 gaaattctgg caccgttatc acgattttgt ccgctgaaca ttgcatccgc tgtaaagcgt
    36241 aaccctgacg gagtttgact aacagtcata tacgcttcag gaataggaaa caattttacg
    36301 gtatgatcca ataattgcgt cccctttgat gcgatttccc ggagcggccg gatatcatcc
    36361 agcacataaa aagatctgcc aaaggttccg atgaccagat catgctcccg gggttgaatt
    36421 accaggtcca ttgtaggaac tgtaggatat ccatttgtcc atttagacca tgtctttccc
    36481 tcatcaatag agacatataa accatattct gtcccaagga aaaccaggcg tttttcaaca
    36541 ggatcctgaa ctacggaaag gctaaatccc catacggtat tttcactgac gaggctttcc
    36601 caggtttgtc cataatcttt tgttctgaac aggtaaggtt tgaaatcgaa atttcggtag
    36661 ttattagcaa caacatatgc ttcgccgcca ctaaaattgg aagccttaac ctggcaaacc
    36721 catgctccct ttggcatcga cgtaattcta cccgcaacat tgatccagga ctttccacca
    36781 tcacgggtaa gctgaacatt accatcatcg gttccaaccc aaatcactcc tttttcaaca
    36841 gcgcttggtt caatagcaag taccgtggtg tagttttctg ctcctgttgc atccatagtt
    36901 aggccaccac tttcatgctg cttttgtttt tcaggttcat tggtagtgag gtcgggtgag
    36961 attatttccc aagatgaacc tttgttcgtg cttttatgta caaactgact accaaaatat
    37021 atagtcgact tatcaaatgg atcctgtgct attgcggagt tccagttaaa acgtaattga
    37081 acgcccggat cgggatgagt tggcttgata actttagtgt ggccactttg gatatcgtaa
    37141 cgccttacat tgccttgctg tgacatagtg tatccatagc ggggattttc aggatcggga
    37201 actacatcaa atccatcgcc aaaagaaact tcctgccagt atgaattcct gattcctcct
    37261 gatttaaaca cataggcagg gccaccccag gaaccattat cttgcatgcc accgtagaca
    37321 ttgtagggaa gttggttgtc cacgttcaca tggtaaaatt ggcctacggg aagattttcc
    37381 gcaaagcgcc acgtcttccc accatcatga gtgatgttca agccgccgtc attcccatca
    37441 atcataaacc cggggttttc cggatgaata taccaggcat gatggtcagg gtgaattccc
    37501 ccaatgtttc cctgcatcag tcttgaccag gtctttccgc catcttcact tacatccacg
    37561 gagctataca aatgataaag ccggttttca ttcctggagt ccacaaaaat atcgttgtaa
    37621 taaaatggac gatttccaat gacgggttca tcattaactt tgctccattt gtatccgcca
    37681 tcctcggagc gatacaaagc atttttcttt gcttcaacaa tggcatatac aatgttgggt
    37741 tttgaatccg ctatggcaag tccaattctc cctaaatcac cttcaggtaa cccattttcc
    37801 gtagattgtt ttgtccacgt ggttccacca tcgagtgtaa catacagacc ggatgagggc
    37861 ccaccggaag taaatgtcca gggttttctt ctgtgctccc acatggctac caaaattttg
    37921 ttaggattgg ttggatcaac taccatatcg gcggcaccag tcttttgatc aacataaaga
    37981 actttctccc aggttttacc accatctttt gttttgaaaa ctcctctttc gggatgatca
    38041 ccccagggtg atccaatgga tgcaacgaac actacatccg gattatcccg gtggatgatg
    38101 atgcggtgaa tgttgcgagt tttttccaaa cccattaaag tccaggtttt tcctgcatca
    38161 aggcttttgt ataatccata tccaccgttc aaactatttc ttggatttcc ttcccctgtg
    38221 cctacccata ttacatccgg attattttgt tgaattgtaa tcgcgcctat tgaagatacg
    38281 gcttccttat caaagacagg ctcccaggca attccgccgc ttgaggattt ccataatccg
    38341 cctgaagcag accccgcgta aataattttc ggatcagaag ccacaacatc aatggcagtt
    38401 atcctaccgg acattcctgc aggaccaata ctccgtgctt tcatgtcttt gaatttgctc
    38461 acctcaagtt gccggcccaa tgacaattct gatgatagca gtatcatcat tagaatagta
    38521 aataagtttc ttagtttcat ggttggtttt tttatatgga aaagtgtaaa tgttttgtga
    38581 agtgatttgg atttcaagtt gaataattaa atctattcga atcagaacta aattgcttta
    38641 tctatttttt aacctgttgg attttgatgt aaagggttct taatttatat gatatgatcg
    38701 agatttgctg aacgaatgtc taagtttcta cgtcagatct ttattttacc cgtcaggttt
    38761 taccaggtag ccataagccc gtaccttggc gcaaactgca ggtatacgcc aacgtgttct
    38821 gaatacacta ttgaagccat taatgaatgg gggcctatga aaggtctatg gcttggaata
    38881 aaacgggttg ccagttgcca tccctgggga ggacacggat atgaccctgt gcctgggaaa
    38941 gaagaaatta agtctacgaa acagacctga taaattcaat ctcggtttcc aaaaccgcaa
    39001 cataattagg gccgataaag tcttccagtt tgtttgaata aggcttgaac tcaattttct
    39061 ttggataaag ttttccaact ctgtatccga gaggttcaaa gaagtcataa taatccatta
    39121 gcagattctt agtaagaata ttcaggtatc cgtattcaaa ctgaacaacc ctgattctct
    39181 tttcgcgcaa acaattttca aaacctttta atgccttcat ttcagctcct tctatatcca
    39241 gcttgaggag gtcaattttt tcaattccaa tggagtgcac ataatcatca cccttaatca
    39301 cttctatgtc aatgatagtt tttgtttcag aagtaatccc attgtgtgag tagagagatg
    39361 aatgcacgct atattcaaac aggttcattg gtatttgctg atttacatcc gaaagtcctt
    39421 ttgttattac aatcacattg gatgaataat taccaatggc ctttttcagc aaatctgaag
    39481 tatccggaac aggctcaaaa caatgtacgg aagcagaggt gcaaattttt gttgcttcaa
    39541 gggcatattc gccgacattt gctccaccat caataattac tttgggatta agattagcaa
    39601 tttttttcaa tagccagact tctccattgc tatgaatatc atgattcctg ttctcgtacc
    39661 acctattcat agatcttcca aagccagcca gaattttttg aactggattg tgggagtttc
    39721 ttttaaacca tcgcttaaga atcttcactt ctgcttttca acactccaat ttacaattat
    39781 ttcaggatca aactggccgc gccgagtatt gatgagtctt gatcctctaa tcccgaaggt
    39841 aatactttca ctgaatcttt aaataaagtc ataagattct tctctaactg aaaaattacc
    39901 tgatcaaaaa ggatactgcc cgcctttgca agcccacctg caaggaaaat agcttcaggg
    39961 gcaaggtgat agacagtatc cgccagtttt attccaagaa ttctgccggt atattcaaat
    40021 gcttcagttg caattttatc tccccttaag gcctccccgg taatcatttc cgccgacagg
    40081 tcattaaagc cgatatttcg cagcacactt tcttccctag aatccgccag tagcttataa
    40141 accgttcgct tgattccggt cgcggagaca tatgtttcca ggcatccctt tttgccacag
    40201 gcacaacttc tgccattaat attcactgtt tgatgtccca gttctccggc aaatccggaa
    40261 tgcccgtaga caatttcacc attcactact ataccgctgc ccaatcctgt tcccatcgtt
    40321 attacaacaa agtttctcat tccctttgcg ctgccgaata gcatttctcc cattgctgca
    40381 gcattcgcgt cattagtcac gaatgttttt gcattcagtg attcttccac caatcggctt
    40441 aagggtacaa ttcctttcca ggcaagattg gttgcctgtt caattgttcc tctatagaaa
    40501 ttaccatttg gagctcctat gccaattcct ataagttcaa tttgttcggg taatgcggcc
    40561 agggcctttt taatttcttt tttcaggtcc gcaataaaaa aaattatgtc atcattggtg
    40621 tttgtgctga attttccttt gctcagaatt tctccggatt tttcaacaaa tccgtattta
    40681 gtatccgtgc caccaatatc tattccaagt gctacttgcc ggctcattat ttcagcttga
    40741 taaaattttt gcaattctcc aggcttcctg atttattatt ttaggagtgg aaatggattt
    40801 ctggaaaggt gtaaatacta ttttgctgtt ttttattccg gccataacat tcgacttgcc
    40861 ctctttaagg gcttctatag cggcaactcc aagtctgctt gccagtagtc gatcaaaaac
    40921 tgtaggagat ccacctcttt gtatatggcc taagatggtt acacgcggtt cgaagtaact
    40981 aaatttttct ttcacttttt ctgcaagttg caatgctccg cctaattttc ctccttcagc
    41041 taccaccaca agactggaag actttttgct cttcaaacct ttattaataa tttccaccag
    41101 ctcttccagg gtaatgtcat cttccggtat tatggttgcc accgcacctc cggcgattcc
    41161 acttcgcatg gcaataaatc cactatctct acccatcact tcaataaaaa ataaccggtt
    41221 atgagataga gcggtgtccc ggatcatgtc aatagctttc acggctgtat tggttgctgt
    41281 gtcatatcct attgtcgcgt ctgtgccaaa caagtcattg tcaattgttc ccgggattcc
    41341 aattatcgga atatcatatt ccttatttaa agttatagca cctctgaaag taccatcacc
    41401 gccaatggct accagggcat caattttatt ttttttcaat ttctcaattg cttttttccg
    41461 gcctgtgcgc gttctgaatt cttcacttcg tgcagattga agtatagtgc cgcctcgatg
    41521 aataatgttt gccaccgacc tgacattcat cttcttcatt tttcccttaa tcataccttg
    41581 ataccctgaa aaaatgccga cgatagcaaa tttataatat ataccggctc taacgacagc
    41641 tcgaattgcc gcattcatgc ccggtgcgtc tccacctgaa gtgaagacac caattttctt
    41701 gattttagac atcctgaaag gttatgatta tttcttaaac aggcaaattc cagccttctc
    41761 ggtattctcg tttaacaaac tgattagctt catcaaaatt agtaattttc atgttggcac
    41821 catcccacat caattgcttt cttcccggat aattgaatgg agcccaatct ccgtatttcc
    41881 ttccgggttt taattctcta atcccgaaac ttcttattgc aaggttgccc atcaggataa
    41941 tttctgataa cggtcccgaa atatcaaaat ttgaaactgc aggctcgcca cccttgcaag
    42001 catttaccca gctttgataa tgaccatctt tcacaggagt ctcgtccgag ggggcagcat
    42061 tttcttccat taatgattta ggaagtaata caggattatt tccataacac tcggtcataa
    42121 ttttgccttt tgtgccttcg aaaattacac cgccatccca actgcccaaa ggatcatcag
    42181 gtcccagttc agcaggccga tccggaagaa ttcctccatc ataccaggta agttttaccg
    42241 gaggtaatcc agtaccccga tcaggaaagt gaaaaactac tttgcatgcc atagggcagc
    42301 tatcatcaaa tatagcttct ttgaagtctg ccacatattg ttcagtaacg ctggcttcca
    42361 ctgaatccgg atgacccagt ttgatggagc gtacaactac atctacaatg tggcatgcca
    42421 tgtctcccaa agcaccagtt ccaaaatccc accaacctct ccatttaaaa ggtaaatatc
    42481 ctgagttgta atctcgcatc tttgaaggac caagccataa attccagtct aattcagccg
    42541 gtatcggatg cttttcagta ggggtaggga taccttgtgg ccatatcggg cggttggtcc
    42601 agcaatgtac attagtaatt tctccgatta ctcctgattc aatccacttg actgtttgcc
    42661 aggttcccgc tcctgaagca ccctggttac ccatttgagt tgccagtttg tttttcctgg
    42721 ccatttctgt catcatcctg acttcataaa cgtcatgagc taaaggcttt tccacataca
    42781 catgtttacc tgatgccatt gcacttaacg ccaccacagc gtgggaatga tcagggatag
    42841 ccaccgtaac cgcatcgata tccttttgct tatctaacat catgcggaaa tccttatatt
    42901 ttggtgcatt cggatgtttg ttataggtat ttgctgattg ccggtcatca acatcacaca
    42961 atgccacaat attattgaga ccctctttat acatttcatt aattactccc gcaccacggc
    43021 ctccggcacc gacagcagca atgttgagtt tgtcactcgg agaaatgaat ccatttccac
    43081 ctaatacata ccttggtaca attataaatc cggctgctgc cagtgatgaa ccttttataa
    43141 aatcgcgccg gttaaatttt ttcctatgaa gtgatttcat tgatttgagg tttaaggact
    43201 tcaatttaaa cataattgct ctggaatcaa tgattaacgt gctttcagga aaaatttgaa
    43261 ttattttgta tccaatttag aatctaatcg tttaacacta tatcgtattc aatttggaac
    43321 attaaaaaaa tgaaacagga aggacttggt gaatttcagg aactgatatt actggcaata
    43381 cttgttttgg aggatgatgc atatggcgtt agcattcaaa aggaaattaa ggataaagca
    43441 aaacggaaag tcagcagggg agcattgcat tcagcgctca caagattgca ggaaaaaggc
    43501 ctggttgtca gcaaaatggg tggtgccact gctgaaaggg gaggcagaag gaaaagattt
    43561 ttcaatctga ctcagaaagg taccagagct gttactttag ctagaatatc cagggtaaat
    43621 ttttaccagg ctatagggaa tcttgatttt gggaaagcct gatgaaaaaa ggaactccgc
    43681 cattatgggc aaggaaattt cttcagtggt ttgctgatcc gaactattat gaagaattgc
    43741 agggcgacct ggaagaaaac tattttaggg atattaaatc gctcggaaca ttaaaagcca
    43801 gatggaatta catttttgaa acatttaaac tttttagacc aagtgtttgg aatcactctt
    43861 tttttacgag tcaaaactca aattatatgt ttagaaatta tgccaaagta acctggagaa
    43921 accttagaaa gcaaaagatt ttctcattca tcaatctttt tggattagct gtaagcttgt
    43981 ccgtttgcct tttgattata agtatcattg tcgatcaact ttcgtttgat aagtttcaca
    44041 agaagtacgc taatatacat cgtattatta cttacaataa tgatttgcgg tatggttggg
    44101 atgagtacct ggcaaccact tcactgccgc tctatgagaa tctaaagaat aatccggttg
    44161 ttggagacat agtcaggatc aaaagaaatt tttggggaga tgttgaaaca ggaaagaaaa
    44221 ccattccctt aagtggtcac atggcagata aggatttttt caaaatattt tcttttgaac
    44281 ttatttcagg aaatccggaa aacgctttag cggcaccttt ttcagttgtt ctttcagaaa
    44341 gttccaaata caaattattt ggtgattcaa aggcactggg tgaaacgata aaagtgggca
    44401 gatatggaga ttatattgtg acaggcataa tgaaagatcc accacggact tcccacatgc
    44461 aatttgatat gatcggctca tttgcaagtc tggaaggtct taaggattcc ggaatctatg
    44521 ccggaaatga caactggtta aattacacag aatactatat ttatattact atccctgata
    44581 aatctgaaac cgaaaccctg gaaaacctgc ttcacagatt tggcgaagta gaaagcaaga
    44641 aaataaataa ccggatctta gactttgaac ttcaatccct ggcggcgatc acacccggga
    44701 gggatcttgc aaaccaaatt ggccctaact ttggaatcgc gcctttgcgg tcgttgatga
    44761 tacttgccct gcttattctt tccctggctt gttttaatta tgttaacctt acacttgcca
    44821 aatccttgaa aagatttaag gaagtaggaa taagaaaagt gatgggtagt caaaagcgac
    44881 agctggttat tcagtttgtg tttgaatcgg tttgcctttc tttaattgca ataattcctg
    44941 cttttgtttt aatggttgca ctaaagcctc aattcttttc tatcgaaccc tcctcaatgg
    45001 ccagatttct gacctttgaa tttactcccg aaataatttt agcaatattg gggttcacca
    45061 ttcttaccgg attacttgca gggataattc cggcattaat tctggccggc gtaaaagcat
    45121 cggcggggtt aaaagacaaa acggtgaaaa tttttaccgg catttcactt cgaaaaggat
    45181 tggtggttgc acaatttggg attgctatat tctttattat tttaaccaat ctggcttata
    45241 aatactataa ttttcaattg gattttgacc tcggatatgc ctacgatgaa atttataata
    45301 tagaattgca aggtacggac catgagatat tccgggctga atttagccag ataccggaag
    45361 tggaaagcat ttccttttcc aattacctcc tttcaaccag cacaaagaac agtaacctga
    45421 ttattaccga aaacaggcaa gacaccatac gctcagttaa tatggcaatt gatgaaaatt
    45481 tcctgtccaa tcataatttc gcaataattg ccgggaacga ttttaatgat ttcactttag
    45541 gaaatgaaaa cagaattatt gtaaatgaga aaatggtttt ggcaataggg aaagccaata
    45601 atgcggatgt ggtcgggctt tctgtttggc tggaaggaaa agaagtaagt atcgccggag
    45661 ttgtgaaaaa tttttatcac aacagatcca gccagcccat agagccgttc tttttcaaat
    45721 acaatccgga taatgtgcac tttgcaaatc taaaattgag aagtaagaat ttgccggcaa
    45781 caatttctaa aatggaaaca gcctggaaga gaattgatcg tgaggtgcac agttataagg
    45841 gagaatttta tagccggcaa attgaagctt ctttatctga actaaaagcg atgataaaac
    45901 tgattggatt ttcggcgtta ctcacaatat gtatttcatt actgggaatt ctgggaatga
    45961 ttatttacac atgcgagacc agggtcaagg aaattggaat cagaaaagta ttaggcgcta
    46021 gtatttctac tgttgtatat actctttcca aaggctatat caccattctt agtgtggctt
    46081 ccattatttc attactactt gtctattttt tatatagtaa aatcatttct cccgagctta
    46141 ataccaaacc tgattttaca tttcttgatt attttggtgg tgtcctggcc gtttccggga
    46201 taggactact aattgttatt ttcaacggtt acctgactgc actaagaaat cctgtgatta
    46261 gtttgaagga agagtagggg atgtatttgc ttctctcaat taatgcttta aaatcaggtt
    46321 attcatatga ctacgatgtc tgattttatc ataaagcatt gataaataaa acgttatggt
    46381 aaaattgaca agttccaatt taatcattaa gttcccgaat tttatacaaa tgcgaaacat
    46441 atcaagacac gttccgtatg tttatacaaa tacgaaacaa atatgaaagg aactaacctc
    46501 ggagaatttg aagaactggt acttttagtc actgccggcc tgggagaatc tgcgtataca
    46561 gtggcgatta cggatcagat taatggcagc actaaaagaa gcgtggtctt aagtgtggtt
    46621 catactgccc ttcacagact ggagaaaaaa ggatttgtga aatcgacact tagtggggct
    46681 acggaagaaa gaggaggaag acgcaaaaga gtttttacaa ttaccagtgc cggaatgaaa
    46741 gcattatcaa attcgaagtc ccagagagac caactttggg aattaattcc ggagattaat
    46801 tttcagaaat aaccaacact tgaatgaaac gtgaagttcc aaaatgggct gatcgattcc
    46861 tgcaatggtt ttgcgaacca tcaatgagag aagatatcca gggagattta tatgaatact
    46921 ttaatcgaag agttgaatct gatggtattc gtatggcacg aattaaatat gtattagatg
    46981 ttttccgttt ttttaaacct cacgttgtta gaaaaccaaa gtttcattat tcaaacccat
    47041 ttaactttat gaaattcaat tttgtacttg ctttaagatc atttttgaaa tccaggctta
    47101 taggtatttt aaaccttgtc gggttaatta ccggaatcac ttgcgctgtt cttatttctc
    47161 tttgggtgat tagtgaatta agttatgatt catttcatgc aaaaagtgac agaattttcc
    47221 gggtgccaaa tacgtttacc agcaaaagtg aaacttttac ccaggcaatt tccggacctg
    47281 ctttaggtgc ccaaatcccc gggctaatct cgggtgtgga agccggcaca agaactatgg
    47341 gtatatggag ccaaataggc atcgatgata agcaatttgt tgaaaagggg gttcttatag
    47401 cagatccaaa tatttttgaa gtgttttctt ttaaaatcct gaagggttca gcacgggagg
    47461 ctctaacgga actcaattcc atcgtactaa cccctgagat ggcgactaaa tattttggta
    47521 ctgaaaatcc gattaataaa acgctggtgc tgggtgggga gagagctatg aaagtaacgg
    47581 cattggtaga aacaccaccg ccaaactccc agcttcaatt tgattttgta atcccggctg
    47641 atttggtgaa agatttttgg tcatgggaaa atatggacga aacctggata ggtggtgcct
    47701 ttcatacgta tgttttgtta aacgatggag tcaatccaaa cgcaatagaa aaagaaatca
    47761 acgatcttgt ttgggagaaa tccggagaaa gacaaagaac aatggatgtt aaatataatt
    47821 attttcttca gccattaact tccattcacc tgcactcaga tttgcggtat gaccactcca
    47881 ataatggcgg tatggaaaat gtttacattt tttccatcgt aggattagct attcttttgc
    47941 tggcgtctat aaactacatt aaccttatag tggccggaag tataaccagg gccaaagaaa
    48001 tcgggattaa aaaagtgctg ggtgcaatgc gatcccaaat tgcagggcag cacctgttgg
    48061 aatcagtttt atctgttctt gtggccaccg cgatcagcct tggcgtcgtt tatttgttac
    48121 ttccattttt tgagactttt acagattctc aatacggttg gcttctgaaa ggagaattct
    48181 ttctgtccat cattgccgga tcactgattt taggaatctt atccgggttg tatcccgctg
    48241 taattttgtc aaggtaccat ccttctgaaa cgctaaaggg aaactttaag aaaggtgcta
    48301 aaggtatgtg gttgcgaaaa actctaatta ttttccagtt tgcagtaaca attactctgc
    48361 tgataagcat cctggtaatt aacaggcaaa tgaaatttat aaatagccag aatttgggat
    48421 taaatcataa agaaatcctt tatgtaaata ttgaaaactc agatccgttg caggagaatt
    48481 ttcaggcatt taggaatgaa ttaatgaata tatcaggtgt tattaatgtt tcaggccaca
    48541 gacgtgctta ccctgtaaat ggtttaagta atggaattac catggtcgaa aattcaaatg
    48601 gggagatggt ttcatcaagt ctataccaca tgtttgtcga ttataattac gctgatacct
    48661 atggtctaaa attactggca ggaagattct tctctgagca gttctcctcg gattccacag
    48721 atgctattgt cgtaaacctc gctgccgtga aaacttttgg atggggtgaa cctgaaaatg
    48781 ccattggtaa gaaatttgga gaggcgccag agcatagatt tgtcataggg gtagtaaata
    48841 atttcaattt tgaaacgctt agaaagaatg tggagccatt acgaatactt cctgtaaata
    48901 ctccaaccga agtttccatc aaagcggagt tttcaaatcc agccgcgttg atttccggta
    48961 tagaaaaagt atggaagaaa tttttaccag gccaggcaat gaatccggca ttcatggata
    49021 atgatttgca aaatcaatac actaccgaac tcaggttcaa gacaattttt gcaatttttg
    49081 ccggaatttc tatcattatc gcgtgtttgg gattatttgg tctggcaact ttttctgttc
    49141 aacaacaaac aaaagaaatt gggattagga aagtactggg agcatccaca gcgggaatat
    49201 taagaatgat taatcaacaa tttctactat atgttgtagc ggcgcttctt tttgcaatcc
    49261 cacttgcaat ttttttgatg agaaaatggt tgaatgggtt tgcttataaa atttctttgg
    49321 agatttccga tttcattctg ggtgcaacca tcgcccttat tgtggccgtg gttactatta
    49381 gttaccacag cctgatggca gcaacaacaa atccggtcaa tactctcaaa gctgaataaa
    49441 ccaaaaagaa aataacacta aaaacctgca gcttcaggat tagccggcgg ggccaaaaga
    49501 aattcttcaa agttggtgta gttataacta attaatcata agaaatataa actcaatgaa
    49561 actaattagc gctcagggtt cgctttaatg accggcgcgc cagcaaactg ctaataagtg
    49621 ataatactgc acccataaga aatggagctc cgggaaaata aatcgaactt gctgacgaag
    49681 taaagtatgc aaacaagttc gtcatcactg gaggtcctac aatgctggta acacttatca
    49741 aactggttaa agcaccttgc agttctccct gctcattcga gttgacttgt cctgccatta
    49801 ttccctgaag ggaaggtccg gcaattcctc ctaatgcata tggtataagg aaggcgaaca
    49861 tcatccaacc ttgagtggca aaagcgaata aagtaaaacc tacacaatag aggcccaa
//
LOCUS       DASWHX010000145         5608 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_2009495, whole genome shotgun sequence.
ACCESSION   DASWHX010000145 DASWHX010000000
VERSION     DASWHX010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 5608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5608
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2009495"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="VGA21_09850"
     CDS             <1..265
                     /locus_tag="VGA21_09850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650853.1"
                     /translation="LLAQIHNNPQLEGPRMPFIVPRELETTWLKEGLDPAEARLMISS
                     FTHEELVAYPVRRLRGKEAVGNNPKAIEKFSYPELSFQQGSLF"
     gene            903..2420
                     /locus_tag="VGA21_09855"
     CDS             903..2420
                     /locus_tag="VGA21_09855"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014021067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HEX9650854.1"
                     /translation="MKDKKKNKKLTTASGKPYVENENTMSVGPRGPLLLQDFILHEKM
                     AHFNRERIPERVVHAKGSGAFGTFTVTHDITKYTKAKLFNKIGKETKVFVRFSTVGGE
                     KGSADTERDPRGFAMKFYTEDGNWDLVGNNTPVFFVKDPKKFSDFIHTQKRDPKTNCK
                     SPTMMWDYWSLNPESLHQVMILMSDRGTPYGHRHMNGYGSHTFSMINSKNERVWVKYH
                     FKTMQGIKNFNNDEATEYKGKDPDFSQRDLVESIEKRDFPKWALKIQVMTDEQAKNFP
                     FNPFDLTKIWPHADFPLIDVGVMELNQVPKNYFADVEQSAFAPAHIVDGIGYSPDKML
                     QGRLLSYPDAHRYRLGVNYEQIPVNKCPYQVANYERDGYMRVDENGGSSPNYFPNSFD
                     DVYTDKSYKEPDMQLDNDFADWYDRNSEAENDHFTQPGWLFNKAMNDYDRHNLVSNIV
                     GAMSGITGEKKELIINRQICHFFRADEKLGMAVAKGLGVDVDKVMAEMKHETVTP"
     gene            complement(2558..3553)
                     /gene="floA"
                     /locus_tag="VGA21_09860"
     CDS             complement(2558..3553)
                     /gene="floA"
                     /locus_tag="VGA21_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654193.1"
                     /note="flotillin-like protein involved in membrane lipid
                     rafts; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin-like protein FloA"
                     /protein_id="HEX9650855.1"
                     /translation="MDSLSLIFIVFGSIVLFFVFLYFVPVNLWITAIFSGVSVGLFEL
                     VFMRIRKVPPRIIVESMITATKAGLKLTTTELETHFLAGGNVPNVIRALISADKANIT
                     LDFKQATAIDLAGRDVFEAVLISVNPKVITTPKVAAVAADGIQLIAIARVTLRANIRQ
                     LVGGAGEDTILARVGEGIVTSIGSAASHKHVLENPDKISKLVLARGLDAGTAFEILSI
                     DIADVDVGANIGAKLQTDQASADLKVAEAKAEERRAMAVATEQEMKAKAQEARAKVIL
                     AEAEIPLAMAEAFRSGNLGIMDYYRMKNIEADTSMRDSISGSGQSKPKKGTTDKG"
     gene            complement(3558..4028)
                     /locus_tag="VGA21_09865"
     CDS             complement(3558..4028)
                     /locus_tag="VGA21_09865"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEX9650856.1"
                     /translation="MIEWISVVAILLLGIGLIIVELIFIPGTTVVGVLGLGFCVGGIV
                     MSFSYFGSAVGFIVLILTGVISVTALVISLRSGVWKKFSNESSIQSKFNEGTNELVKI
                     GDEGLTLSTLKPYGKVEFNDKEFEVKTNGNYLERGVKVKIIHKDGNQIIVEPLK"
     gene            complement(4045..5520)
                     /gene="proS"
                     /locus_tag="VGA21_09870"
     CDS             complement(4045..5520)
                     /gene="proS"
                     /locus_tag="VGA21_09870"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019599184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEX9650857.1"
                     /translation="MSKGITTREADYSQWYNELVKKADLAENSPVRGCMIIKPYGYSI
                     WEKMQAGLDKMFKETGHTNAYFPLFIPKSFLSKEADHVKGFAKECAVVTHYRLKNDPN
                     GKGIVVDPEAKLEEELIIRPTSETMIWSSYKNWIQSYRDLPLLLNQWVNVVRWEMRTR
                     LFLRTTEFLWQEGHTAHATKQEAVDETIQMMNVYAKFAEDYMAMPVIKGVKTESEKFA
                     GAVDTYCIEALMQDGKALQAGTSHFLGQNFAKAFDVKFADKEGKLEYVWGTSWGATTR
                     LMGALVMVHSDDNGLVLPPRLAPIQVAIVPIYKNEGELKAVSEKANIIRKALEAEGIS
                     VKYDDRDTQKPGWKFAEYEFKGVPVRLAMGPRDLENNTIEVARRDLLQKESISQDGIV
                     VHVKKLLEEIQKNLFDRALERREKQTYSVDTWEEFVETLDKKGGFIRAHWDGTPETEA
                     QIKEKTKATVRCIELDAKEESGKCILTGKSSSKRVLFAKAY"
BASE COUNT         1624 a         1230 c         1117 g         1637 t
ORIGIN      
        1 attattggcc cagattcata ataacccgca gttggaaggt ccacgcatgc catttattgt
       61 tccgagggaa ctggaaacga catggcttaa agaaggcttg gatccggctg aggcaagact
      121 aatgattagt agttttaccc atgaagagtt ggtagcttat ccggttcgca ggttacgcgg
      181 aaaagaggcc gttggcaata atccaaaagc aattgaaaaa tttagttacc ctgaattatc
      241 ttttcagcag ggaagtctgt tttagtaagg ctggttaaca acgtagagaa atggtttgag
      301 agttttcagc ttaaatccgc gtgatctgga gggaatactt ttttaaatcc cgctgatagc
      361 gcagatcacc gcggaattga ttcactgagt cttctgcgca aatccgcgaa atctgcggga
      421 gaaaaggagt tttttctgac caaaatctgc gtcgatttta tagagaaaat tttttaaaat
      481 cccgatgaca acgcagtcac cgtggaattg attcactgag tcttctgcgc gaatccgcga
      541 aatctgcggg agaaaaggag tttttctgac caaaatccgc gtcgatttta tagagaaaat
      601 tttttaaaat cccgatgaca acgcagtcac cgcggaattg attcactgag tcttctgcgc
      661 gaatccgcga aatctgcggg agaaaaggag ttttttctgc ccaaaatccc agtcgatctt
      721 tgaaagaaaa gtttattgag tcccgttgat agcgcagatc accacggaaa gaaaagtgta
      781 atactttttt aagttgttgt aatgtcctat ctatattatc tatataaaga taaagattat
      841 cagtttgatt tatacaattt aacgcgttac cttgaactaa taaattatac aacattcaac
      901 gcatgaaaga caagaagaaa aataagaaat tgacaacagc ctcaggcaaa ccttatgtgg
      961 aaaatgagaa tactatgagc gttgggccca gaggtcccct gttgcttcag gatttcattc
     1021 tacatgaaaa aatggcacat tttaatcggg aaagaattcc tgagcgggtt gtacatgcaa
     1081 aaggaagtgg agcctttgga acatttaccg taacccatga cattacaaaa tatactaaag
     1141 ccaaactttt caataagata gggaaagaga ctaaggtctt tgtaaggttt tctacagtag
     1201 gaggagaaaa gggtagtgcg gatactgaaa gagatccacg aggctttgca atgaagtttt
     1261 acacagaaga cggaaactgg gatcttgtgg gtaataatac gcccgtcttc tttgtaaaag
     1321 acccgaaaaa gttttctgat ttcattcata ctcagaaaag agatccaaaa acaaactgca
     1381 aaagcccgac aatgatgtgg gattattgga gtttaaatcc tgaaagtcta caccaggtga
     1441 tgatcctgat gagcgacaga ggtacgccgt atggacacag gcatatgaat ggttatggtt
     1501 cacacacctt cagtatgatc aatagtaaga atgaaagggt atgggtgaaa tatcatttta
     1561 agaccatgca ggggataaag aactttaaca acgatgaagc aaccgaatat aaaggaaagg
     1621 atcccgattt ttcacaacga gatttggtag aatcgattga gaaaagggat ttcccgaaat
     1681 gggccttgaa gattcaggtt atgacggatg aacaggcaaa gaattttcct ttcaatcctt
     1741 ttgatcttac aaaaatttgg ccacatgccg acttcccgct aattgacgtc ggtgtgatgg
     1801 agctgaacca ggtaccgaag aattatttcg ctgatgttga acaaagtgca tttgcccccg
     1861 cgcacattgt tgacggaatc gggtattctc ctgataaaat gctccaggga agattgcttt
     1921 cttatccgga tgcgcatagg taccgtttgg gagtaaatta tgaacaaatt cctgtaaaca
     1981 aatgtcctta ccaggtagcg aattatgaaa gagatggcta catgagagtg gatgaaaacg
     2041 gcggaagctc accgaattat tttccaaaca gctttgatga tgtttacaca gacaaatcat
     2101 ataaagagcc tgacatgcag ttggacaatg attttgccga ttggtatgat cgaaatagtg
     2161 aagcagaaaa tgatcatttc acgcagccgg gttggttatt caataaggct atgaatgatt
     2221 atgaccgtca taatttggtt tctaatattg taggagccat gagcggtata acaggcgaaa
     2281 agaaagagtt gataataaac agacagatat gtcatttctt ccgtgcggat gaaaagctgg
     2341 gcatggctgt agccaagggc cttggtgtgg atgttgataa agtgatggct gagatgaaac
     2401 acgaaaccgt cactccttaa ttggacaaag gagagcattc tgattgttct cctttttaca
     2461 ttctataatt aaaattcccc ggcaaattca actacaaaaa ccctgatgtg tttctctatc
     2521 tctccaaaaa attactgatt ccggttttac aaattaacta gcctttatct gtggttccct
     2581 tcttgggctt cgattgtcct gagccagaaa tggaatcccg catggatgta tccgcctcta
     2641 tgtttttcat ccggtagtaa tccatgatgc cgagatttcc ggaacggaag gcttcggcca
     2701 tggccagagg aatttctgct tctgccaaaa taacttttgc acgtgcttcc tgcgctttgg
     2761 ctttcatctc ctgctctgtt gcaacggcca tagctctccg ttcttccgct ttagcttcgg
     2821 caactttcag gtctgcgctg gcctgatcgg tctgcagttt ggcgccaatg tttgctccca
     2881 catcaacatc cgctatatct atggatagaa tttcaaacgc agttcccgca tccaagccgc
     2941 gagccaatac cagtttggaa attttatcag ggttttccaa cacgtgctta tgagaagcgg
     3001 cggaaccaat agaagtaaca ataccctcgc ctacccttgc cagaatagta tcttctccag
     3061 caccaccaac caattgccgg atattcgccc tgagtgtaac cctggctatc gcgattagct
     3121 gaataccatc tgccgcgacg gcggcaactt tgggagttgt aattactttc ggattaacgg
     3181 aaatcaggac tgcctcaaaa acatctcttc cggccaaatc aattgcagtt gcctgtttga
     3241 agtccagggt aatattcgct ttatcagctg aaatcaaagc ccggataaca ttgggtacat
     3301 tacctccggc aaggaagtgg gtttccaact ccgtagtggt gagcttaagt cctgcttttg
     3361 tggcggtaat cattgactcc acaattatgc gtggtggaac tttcctgatt ctcatgaata
     3421 cgagttcaaa caaacccaca ctaactccgg agaaaatcgc agtgatccag agattaaccg
     3481 gtacgaagta caagaacacg aagaaaagta ctatcgaacc aaagactata aaaattaatg
     3541 ataaactatc catttcttta ctttaaaggt tctactataa tttgatttcc gtctttatga
     3601 attattttta ctttaactcc tctttcaagg tagttgccat ttgtcttaac ttcaaattct
     3661 ttatcgttaa attcaacttt gccgtatggc ttcagtgttg aaagagttaa tccttcatca
     3721 ccaatcttta ccaattcatt ggtgccctca ttaaatttac tttgaatgga ggattcatta
     3781 ctgaattttt tccatactcc ggaacgtaaa cttatcacaa gggcagtgac cgaaattaca
     3841 ccggtgagga tcagcacgat aaaaccaaca gcggacccaa aatagctgaa actcatcact
     3901 attcccccca cgcaaaatcc aaggcccagc actccgacca ctgtggttcc agggataaaa
     3961 atcaattcaa ctattattaa tcctattccc agcagcagaa ttgccactac cgatatccat
     4021 tcgatcatgg gtactaagtt acatctaata tgccttagca aacaagactc ttttactgga
     4081 agattttccc gttaaaatgc attttcccga ctcttccttt gcgtccagct ctatacaccg
     4141 aacagtagct ttagtcttct ccttaatttg agcttcagtt tccggagtgc catcccaatg
     4201 cgcccggatg aagccgcctt tcttgtctag tgtttccaca aattcttccc atgtatccac
     4261 agaatacgtt tgtttttctc ttcgttccaa tgctctgtca aaaagattct tctggatttc
     4321 ttccaaaagc tttttcacat gtactactat tccatcctga cttattgatt ctttctgaag
     4381 caaatcccgc cgggccactt caatggtatt attttcaaga tcccgcggac ccattgccag
     4441 gcgaacggga acacctttaa attcatactc agcaaacttc caccctggct tttgtgtatc
     4501 tctgtcatcg tatttcactg agattccctc cgcttccaac gctttcctga ttatattcgc
     4561 cttttcactt actgctttca attctccttc attcttataa ataggaacga tagccacctg
     4621 gatcggtgcc aatcttggag gcagcaccag gccattgtca tcagaatgca ccatcaccag
     4681 ggctcccatt aacctggtgg tggctcccca ggaagttccc cagacatatt cgagttttcc
     4741 ctccttatcg gcaaatttca catcgaaggc tttggcaaaa ttctgaccca ggaaatgact
     4801 ggttcctgcc tgcaatgctt tgccatcctg catcaatgct tctatacagt aagtgtctac
     4861 cgctccggca aatttctcag attcagtttt tacacctttg attaccggca ttgccatata
     4921 atcctcggca aactttgcat aaacattcat catttgaatg gtctcatcaa ctgcttcttg
     4981 cttcgttgca tgagcggtat gaccttcctg ccataaaaat tcagtagtcc ttagaaataa
     5041 ccgcgtacgc atttcccacc gcactacatt aacccattga tttaacagca aaggcaaatc
     5101 gcggtaagat tggatccagt ttttatatga actccaaatc attgtttcag aagttggcct
     5161 gataattaat tcttcttcca gctttgcctc cggatctacc acaattcctt ttccgtttgg
     5221 atcattcttt aaacgataat gagtgaccac cgcgcattcc ttggcaaatc ctttcacatg
     5281 atctgcttct ttgcttaaaa aagattttgg gatgaataaa ggaaaataag cattggtatg
     5341 accggtttcc ttaaacattt tgtctaaacc tgcctgcatt ttctcccaaa tggaatatcc
     5401 ataaggtttt ataatcatac agcctctcac tggtgaattt tcggccagat ctgccttttt
     5461 gacaagttca ttgtaccatt gtgaataatc cgcttccctg gtcgtaattc ccttactcat
     5521 tttgactttg gcatagtttt ggacttgtat taaaacggag gacaaatata aagtatttat
     5581 gcgcctccca gcgattaaaa taatcgta
//
LOCUS       DASWHX010000146        13890 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_2735142, whole genome shotgun sequence.
ACCESSION   DASWHX010000146 DASWHX010000000
VERSION     DASWHX010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 13890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13890
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2735142"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..453)
                     /locus_tag="VGA21_09875"
     CDS             complement(<1..453)
                     /locus_tag="VGA21_09875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013455242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX9650858.1"
                     /translation="MQKAYKVFKNSCGIFFLFLLACGSAEEKNLADIVFINGVIYTVD
                     ESNPVAEAVAVRDGKILFVGSNLEIDEYTGEQTTQIDLGGKTMTPGFIEGHGHMMGLG
                     YNELNLDLSGVKNYDELVSHVADAVAKANPGDWIVGRGWHQSKWDPVPE"
     gene            506..1201
                     /locus_tag="VGA21_09880"
     CDS             506..1201
                     /locus_tag="VGA21_09880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650859.1"
                     /translation="MLKNKLTITLIFIAFLIPQDSKSQSIGVSFSYFLPKDGYFSAPI
                     SPFSFRGLGFDVTNWFALETGITLYRMSGLKVKGVPFESKKPFVGPNFTFLVPVEGVI
                     QIVGSSQEFKLRGGVFFFVGAGTKLNEGNIDRAIREYEAWDVANSNFDFENHPGWGTH
                     FGAEYVVYFKDQFGISFGANYFIGDAKLNLAGSYTGFEPLQGLTTRNVEFQDSKIDFT
                     GFEISLGLLFSNQ"
     gene            1254..2567
                     /locus_tag="VGA21_09885"
     CDS             1254..2567
                     /locus_tag="VGA21_09885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650860.1"
                     /translation="MFKKSFVLLLFIFLIGCGQSEKESDKNLTVSQIGERYVKLILRI
                     GQFDSDFVDAYYGPENLKPIPISDNQKRDLPSDEFVWEIHHLIHLLEDIDDAKFDELD
                     KAHKTFLMKQLMAAKIKVSMLGGSKLKFDEESKMLYDAIAPHHSEEYFDSLLTELDLA
                     LPGDGSIQARYKSFTDNFIIPKTKIDEVFRIAIAEARRRTGDKLELPLEENFELEYVT
                     DKSWSGYNWYQGGYRSIIQINTDFPVYVDRVIELASHEGYPGHHVYNMMLEKHLVHDK
                     GWVDFSVYPLFSPQSLIAEGSANFGIEMVFPEKERVEFEKRVIYPLAGLDSSQAELYA
                     HIQMLRGKLDYAGNEAARNYLNGEITREEAAAWLEKYNLSSQERALQRTRFFDQYRSY
                     VINYNLGRDLVKDYVIKKTGEDLEKRWEVFEELLSTPKTASMLKN"
     gene            complement(2564..2896)
                     /locus_tag="VGA21_09890"
     CDS             complement(2564..2896)
                     /locus_tag="VGA21_09890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650861.1"
                     /translation="MKPVILLLEAAQIRISTVFPSAEISEVEESLIELIKSGRLPISP
                     NNVELIPQNEDVSMKDIINQKSKDADLVMIGFRKEAIKQLGDTLFEGYDEVGNILFVN
                     TSREKAIY"
     gene            complement(2820..3209)
                     /locus_tag="VGA21_09895"
     CDS             complement(2820..3209)
                     /locus_tag="VGA21_09895"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650862.1"
                     /translation="MERLSKSKANLGTSPVFLTAISTILGAIMFLRFGYAVGIVEFLE
                     TIRIILFAHLVTIPTAMAIAEIATNQKVEGGGEFYIISRSFGINIGASQGEAFDPIYN
                     WVNETCNLVIGSGANSNFYRIPVCGNF"
     gene            complement(3289..4200)
                     /locus_tag="VGA21_09900"
     CDS             complement(3289..4200)
                     /locus_tag="VGA21_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013452615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005254 - chloride channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family ion channel"
                     /protein_id="HEX9650863.1"
                     /translation="MIRYKTTDWLALIFKSYSKEVLRKLLPSLILIGIYTAGIVYLVN
                     DYWQFNFVSTTEVHSILGIVLGLFLVFRMNSAYDRWWEGRVQWGNLVVTSRNLAYKLN
                     AYIPADDSVNRDWLKMMITNFPLSMKEHLRGGVKFEELRFPNEKIEEVLRSSYGVPGA
                     IVGLIYERLNVLIKSGTISGEQLITIDKELSVFSVVLGSCERIRNTPIPYSYSMYLKK
                     FIFTFLITLPFAFVTEFEYFTIPIVMFLFFILVSIELIAEEIEDPFGNDENDLPTNEL
                     TFTIMRDVSKAFEMGDEILPRKIEELQ"
     gene            complement(4358..5704)
                     /locus_tag="VGA21_09905"
     CDS             complement(4358..5704)
                     /locus_tag="VGA21_09905"
                     /inference="COORDINATES: protein motif:HMM:NF019592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HEX9650864.1"
                     /translation="MTTMKNFEINKISKILILLIMPVFFSACEINDLSDPNGPSLSAI
                     SQNATIGEIRNIAIGTENLMRDQIGFYYDVTSIIGREYYFFTNSDPRYTGELLGKGAS
                     TLDNAGFYGTRPYQGRYRVVRNAEILLTTLETTSEVISAEARNGYAGFAKTCQAYSLL
                     LALNLQYQNGIRTDVKDPDNLGPFRSYADALGDVSTLLDDAFTSLQGAGTEFDFPLSS
                     GFSGFDTPSTFGQLNRAIKARVELYRGNKGAALTALTSSFMNLAGSLNAGPSHFYSDL
                     GTEIANPVFRTPDQSEATIAHPSFLTDILPGDNRAGKVTLRPSGTRTLDGLAGNYDVT
                     IFTSFNSPIPIIRNEELILIYAEANIGTDNAEAVNALDIIRNAHGLGGYAGGLTDNEV
                     TNEMLYNRRFSLFGEGHRWIDLRRFNLLNTLPIDRTDDDVWDQFPRPVNEVGVQGG"
     gene            complement(5707..8664)
                     /locus_tag="VGA21_09910"
     CDS             complement(5707..8664)
                     /locus_tag="VGA21_09910"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HEX9650865.1"
                     /translation="MKRISVILLTSLCLLLPLTIFGQERTVTGKVTDGKTGEGLPGVN
                     VLIKGTSSGTITDIDGNYRINLSNENVTLAFSFIGYLSQEIQVGSSNAVDVSMSEDIT
                     SLEEVVISGLASTVKRSNLASSVASISADQLAGMTNQSTLDGALYGKLKGANITASSG
                     APGGGYSIRMRGLTSINGSNEPLIILDGVYLDNSEVNNGQNVVSLAAAAGNPLQQDQP
                     SNRLADINPDDIESVEILKGASAAAIYGSRASGGVIIITTKKGKQGKTQVELSQAIGQ
                     TSIMNPLGVRTFDATKVTTHFGAGEVANFNSAVSSGQLHDYEDELFGNKGLLLNSRIT
                     ISGGSENTSYFLGFSRKDDEGIIKNTGYEKNSFRLNVDHKVTDWLSLNASTNYVLSSA
                     DRGFFGNDNSGTTMGIALSATPPWAQLQPDANGNYPNNPYAASNFLQTRDLMTNNETI
                     NRFILGGQATARIINSSSHGLRLILQGGIDYYNMATTVLFPNTLQFQKDGNGLGGVSA
                     QGNTESNNKNYSAFLVHDFFPAGSPFTFTTQVGITFIDFDRNTIRGTASNMNGSQTNL
                     DQAGSQSILQDRVIQHDRGFFVQEDVNFRDAIIVSLGVRGDKSSNNGDPNKLFYYPKA
                     SLAANIHEFVTFPNAVNQLKLRAAFGQAGNFAVFGDKFTSFAGAVIDGNSGIVIGTTR
                     GNSEVEPERQTEVEFGFDAAFMSNKVALDFTYYVKTVTDLLLTADLPTSSGFASIVTN
                     AAEMQNKGFEIGLLLNLVNTSTIKWTSSNNFWRNTSEVTKLDVPSYTSGGFADFLGQY
                     RIKQGHSPTEIIGVGATPDEDGLVVFGDAQPDFEMSFLNTVEFKGFELNMLWHWKQGG
                     ELINLTALLTDLGGTSPDYDKIDLDPSGVLGNGDYRLGELGSNSGPYIENAGYARMRE
                     IGLYYNIPKSLIGPLSNVKVGFSGNNLLNFFEYRSYDPEVSNFGGKGLSTGVEVLPFP
                     SSKRWNFHLAVKF"
     gene            complement(8777..9817)
                     /locus_tag="VGA21_09915"
     CDS             complement(8777..9817)
                     /locus_tag="VGA21_09915"
                     /inference="COORDINATES: protein motif:HMM:NF013859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650866.1"
                     /translation="MKKIISVSIIFIVVPGIYIYYILSGTGFFREIEYKPYGEVFQEI
                     PIVGAEDFAISYEDSFMIISATDRMAIKRGEPAFGGLYYLDLTASIFQPQLLTANIDK
                     SFNPHGISIWKLDSSNYKVFAINHAGGKHSIEVFLLSDETLFHQETLEDEWLKDPNDL
                     VLVSENQFYLTNDHGYKSRIGVFLESYLGLEVSSVVYFDGESFSLAADGISYANGIAY
                     NSKSAKLYVASSRWFLIREYKVSEDGSITFLKDIPAGTGVDNLEFDNHGVLYSGCHPN
                     LIAFTRYAQLKAENAPSEVVRIKLHGENPEVECIFTDDGNLINASSVAAPFGNYLFVG
                     TVMDDKLLVLKR"
     gene            9939..12662
                     /gene="leuS"
                     /locus_tag="VGA21_09920"
     CDS             9939..12662
                     /gene="leuS"
                     /locus_tag="VGA21_09920"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015265936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEX9650867.1"
                     /translation="MAEYNFREIEKKWQAYWSDNQTFATKEEKGKPKYYVLDMFPYPS
                     GKGLHVGHPLGYIATDIVARYKRITGFNVLHPMGFDSFGLPAEQYAIKTGQHPQVTTA
                     NNIERFKEQLSILGFSYDWSTEIRTSDVDYYRWTQWIFLKLFNSYFDEKEKKAKPIAE
                     LKVPGELIGKARDAYIDNHRLAYQDEIMVNWCPELGTVLANEEVVGGVSEVGGYPVIR
                     KPMRQWMMRITSYADRLLQDMEELEWPESIKLSQKNWIGRSVGADIYFTEKSTGKKIQ
                     VFTTRPDTIFGATYIVLAPEHSLVAQITTDDQKEKVEGYVEKAAQKSDLERAELDKTK
                     TGVFTGSYAINPVNNEELPIWISDYVLISYGYGAIMAVPAGDQRDFEFAIKFNLPIRE
                     VVSKSGKESSELIEAYSEPGIIINSGSYNGMASTDFFDVIVKWLEENDHGKGAVNYKL
                     RDWVFTRQRYWGEPIPIIHTEDGRVVGVPEDQLPVVLPEVESYTGTMDGLSPLAKAVD
                     WVNTINPEDGRPAKRETNTMPQWAGSCWYYLRYLDNKNENEFVPKEKENYWLPVDLYV
                     GGAEHAVLHLLYSRFWHKVLFDLGYINSKEPFRKLINQGMISGKSNFVYRIQGSNKYV
                     SLGLKDQFESDPIHVDVNIVNDDILDLEAFKAYSSLNNDAEFVLEDGKYVCGFAIEKM
                     SKSLLNVVNPDTLVEKYGADTFRMYEMFLGPLEQAKPWNTNGIDGVWKFLKRFWNLYH
                     DESGKLSLSGEDPSQEELKILHKTIKKIKQDIERYSFNTAVSAFMICVNELTPLKCNK
                     EKILQPLLITLSPFAPHLAEEIWAEMGNTTSISFTRFPDYDETHIQEDSFEYPIAING
                     KMRVKMKFALDMPSEDIVKMVLASEIVKKWTDGKTPRKVIIVPKKIVNIVV"
     gene            complement(12664..13221)
                     /locus_tag="VGA21_09925"
     CDS             complement(12664..13221)
                     /locus_tag="VGA21_09925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650868.1"
                     /translation="MQKDHFSYFVDSIPRTDIAKVYKNKFGIGQEPFIATVINKIPPP
                     IHSPFAKINTIEPVVIYPELPDSLEFLGDELMAAELDIPDSSAFKPDTTEVARNWHFN
                     IDQENYFNYLIKKLKIPIEPVSTGAAELEPDTSAIETEGVEGSGEKKGFLKGLFKRKK
                     KESQSEEGTEPNQQDPKQNIGGEPE"
     gene            complement(13425..13787)
                     /locus_tag="VGA21_09930"
                     /pseudo
     CDS             complement(13425..13787)
                     /locus_tag="VGA21_09930"
                     /inference="COORDINATES: protein motif:HMM:NF016152.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
     gene            complement(13784..13876)
                     /locus_tag="VGA21_09935"
     CDS             complement(13784..13876)
                     /locus_tag="VGA21_09935"
                     /inference="COORDINATES: protein motif:HMM:NF042817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6728 family protein"
                     /protein_id="HEX9650869.1"
                     /translation="MKVMHGINRISILVFLGALIIFLIRRFLMG"
BASE COUNT         4256 a         2815 c         2718 g         4101 t
ORIGIN      
        1 ttccggtaca ggatcccatt tgctttgatg ccagccacga ccaactatcc aatcaccggg
       61 attagctttt gcaactgcat cagcaacgtg gctaaccaac tcatcataat ttttcacgcc
      121 gcttaaatcc aggttaagtt cgttatatcc caatcccatc atatgtccat gtccttcaat
      181 aaaacccggc gtcattgtct taccgcctaa atctatttgg gtcgtttgct cacctgtgta
      241 ttcatcaatc tctaaattgg aaccaacaaa aagaattttt ccatccctta ctgcaactgc
      301 ctcagcgaca ggattacttt catcaacggt atatattact ccgtttataa aaacaatatc
      361 cgccagattt ttctcctcag cagaaccgca tgccaataaa aagagaaaga atatgccaca
      421 actatttttg aatactttat aagccttttg cataagtttg ggtgtaattt agatcatttg
      481 tgaaaagaat aaacatacta attctatgct taaaaacaaa ctgacaataa ctctcatatt
      541 cattgcattc cttattcccc aggattccaa gtcacagagt attggtgtat cattttctta
      601 tttcctgccg aaagatggct acttcagtgc accaataagt ccgtttagtt tcagaggcct
      661 gggatttgat gttaccaatt ggtttgcatt agaaaccggc attacccttt acagaatgtc
      721 aggattaaaa gtgaaaggag ttccgttcga atccaaaaaa cctttcgttg gtccaaactt
      781 tacttttctg gttccggttg aaggagtaat tcaaatagtc gggagtagcc aggaatttaa
      841 gttgagaggt ggtgttttct ttttcgtggg ggcggggaca aaattaaatg aaggtaacat
      901 tgaccgggct atcagagagt atgaagcgtg ggacgtagca aattctaatt tcgactttga
      961 aaaccacccg ggatggggca ctcacttcgg tgcagaatat gtagtttatt ttaaagatca
     1021 atttggtatc tcgtttggtg ccaattattt tattggagat gcaaaattga atttagccgg
     1081 gtcatacacc ggtttcgaac ctttgcaagg tttgaccacc agaaatgttg aatttcagga
     1141 ttctaaaatt gattttacgg gttttgaaat ttcactgggt ttattattca gtaaccaata
     1201 acttgcctgg ctcgtaatat atattaaacc ctttacctaa ccctgcttac ttcatgttta
     1261 agaaatcttt tgtattgctg ctattcattt ttttgattgg gtgtggtcaa agtgaaaaag
     1321 aatctgacaa gaatttgaca gtaagtcaaa ttggtgagcg ctatgtgaaa ctcattctta
     1381 gaatcggaca atttgattct gactttgttg acgcctatta tggccctgaa aacctaaaac
     1441 cgatacccat ttctgataat caaaaaaggg atcttccctc tgatgaattt gtttgggaga
     1501 ttcatcattt aattcatctt ctcgaagaca ttgatgatgc taaatttgat gaattggata
     1561 aagctcataa aacctttcta atgaaacaat tgatggcggc caagattaaa gtctccatgc
     1621 ttggcggatc aaagcttaaa ttcgacgagg agtcaaaaat gttgtatgat gccattgcgc
     1681 cacatcattc cgaagaatat tttgacagcc tcttaaccga acttgatctt gccctgccgg
     1741 gagatggatc aattcaggcg aggtataaaa gcttcactga taattttata attccgaaaa
     1801 cgaaaataga tgaggtattc agaattgcaa tcgcagaggc tcgcagaaga accggagaca
     1861 aactggaatt gcctttggaa gagaattttg aattggaata cgttacagat aaatcatggt
     1921 cgggatacaa ttggtaccag ggtggatacc gatctatcat tcagataaat accgattttc
     1981 ctgtttatgt agaccgggta attgagctgg caagtcatga gggctatccc ggtcaccatg
     2041 tttataacat gatgctggaa aaacacctgg tgcacgataa gggctgggtt gatttctcgg
     2101 tgtatccatt atttagtcca caatctttaa ttgccgaagg gtccgctaac tttggaattg
     2161 aaatggtttt cccggaaaag gaaagagttg agtttgaaaa aagggtcata tatccacttg
     2221 cgggtttgga ttcttcacaa gcagaacttt acgcccatat ccagatgtta agagggaaac
     2281 tggattatgc gggaaatgaa gcggccagaa attatttgaa tggtgaaatt acccgggaag
     2341 aggcagcagc ctggctggaa aaatataacc ttagctccca ggagcgggct ttacagcgaa
     2401 caagattttt tgatcagtat aggagctatg ttattaacta caatttgggt agagatttag
     2461 tcaaagatta tgtgattaag aaaacgggag aagatttaga gaaacggtgg gaagtgtttg
     2521 aagaattgct gtctacgccc aagacagcat caatgctgaa aaactaatag attgcttttt
     2581 ccctggaagt attaacaaaa agaatatttc caacctcatc atacccttcg aacaaagtat
     2641 ctcccagttg tttaattgct tccttacgga atccaatcat gacgaggtca gcatcttttg
     2701 atttttgatt gatgatatcc ttcatactta catcctcatt ttgaggaatt agttcaacat
     2761 tattaggcga aattggaagt cgtcctgact taatcagttc aataaggctc tcctctactt
     2821 cagaaatttc cgcagacggg aatacggtag aaattcgaat ttgcgccgct tccaataaca
     2881 agattacagg tttcatttac ccaattataa atcgggtcaa aagcctcgcc ctgggatgct
     2941 ccaatattaa ttccgaatga tcgtgaaatg atataaaact caccaccgcc ttcaactttt
     3001 tgattggtag caatttctgc tattgccata gcggtaggaa tggtaacaag atgggcaaat
     3061 agaataatcc taatggtttc taaaaattcc acaatgccaa ctgcatagcc gaagcgcaaa
     3121 aacataatag cacccagaat ggtggatata gcagtcagaa aaaccggtga tgtgcctaaa
     3181 ttggctttac ttttcgacag tctttccatg ggataacttg aatgtcgaag ttagttctgt
     3241 gtcctaataa aacaaaggga ttgaattcta aaagttaaat gatgcagtct attggagttc
     3301 ctctattttt cttggaagga tttcatcacc catttcaaaa gccttgctta catccctcat
     3361 tattgtgaaa gtgagttcgt tcgttggcaa atcattctcg tcatttccaa agggatcttc
     3421 gatctcttcc gcgattaatt caatgcttac gagaatgaaa aaaagaaaca ttacaatggg
     3481 tattgtaaag tattcgaatt cagtaacaaa agcaaaaggt aaagttatga gaaatgtaaa
     3541 aatgaatttc ttcaaataca tgctgtatga atagggaatt ggagtattcc tgatcctttc
     3601 gcatgagccc agcacgacgg aaaatacgct aagttcctta tcaatggtta ttaattgctc
     3661 gccactgatt gtacctgatt taattaagac attcaatctt tcataaatta agcctactat
     3721 agcacccgga acaccatatg aactacgaag cacttcttct attttttcat tgggaaatct
     3781 taattcttca aacttcacac ctccgcgcaa atgttctttc atagaaagag gaaaatttgt
     3841 tatcatcatt tttagccagt ctcgattgac tgaatcatcg gcagggatat aggcatttaa
     3901 tttatatgcc aggtttcggg aagttactac aagattaccc cactgtactc ttccctccca
     3961 ccaacgatca taagccgagt tcatcctaaa gacaagaaac aaacccagaa caattcctaa
     4021 aatagaatgg acttcggtag tacttacaaa gttaaactgc caatagtcgt taaccagata
     4081 aacaattccg gcagtataaa tgccaattaa gatgagggaa ggaagaagct ttctaagaac
     4141 ttctttactg tatgatttga atattaaggc gagccaatcg gtcgttttgt atcggatcat
     4201 tgtccaaaat atgatttttt aaagataaac tacaaatcat tcaattccta acttagaata
     4261 tgacaatatt ttaggaagga tttatttttg aaacagtaat tataagccag gaataattcg
     4321 gatggcccgg gaaggccatc cgaattgtaa aagaaattta tccaccctga actccaactt
     4381 catttaccgg tcgcgggaat tgatcccaaa catcatcatc tgttctgtca atgggaagag
     4441 tgttcaataa attaaacctt cgcagatcaa tccaacgatg accttctcca aataatgaaa
     4501 atctccggtt atagagcatc tcattggtaa cttcattatc agtcagtcca ccggcgtacc
     4561 ctcccaatcc atgagcattc ctgataatat caagtgcatt aactgcttca gcgttatcag
     4621 ttccaatatt agcctccgcg taaattagaa ttaattcttc atttctgata ataggtattg
     4681 gggaattaaa cgaagtgaat attgtaacat cgtaatttcc tgcaagtcca tcaagagttc
     4741 ttgttcctga gggcctcaac gttacttttc cggccctgtt gtcacctgga aggatatctg
     4801 tcaaaaatga cggatgagca attgtagctt ccgattgatc aggtgttctg aatactgggt
     4861 ttgctatttc agttcccaga tctgagtaaa aatgagaagg acccgcatta agtgaacctg
     4921 ccagattcat aaatgagctt gtaagagcag ttagtgccgc tcctttgttt ccacggtaca
     4981 attcaactct tgctttaatt gcgcggttaa gctgtccgaa agttgaggga gtatcaaaac
     5041 ctgaaaatcc acttgataaa gggaagtcaa attcagttcc tgctccttgc aacgaagtaa
     5101 aagcatcgtc aagcaaagta gaaacatccc ccagggcgtc tgcataactc cggaacggac
     5161 ctaaattatc aggatccttt acatcggttc taattccatt ttggtattgc agattcaatg
     5221 ctaaaagcaa tgaatacgcc tgacaggttt tcgcaaatcc ggcatatccg ttcctggctt
     5281 ctgcagagat cacttcagaa gttgtctcaa gtgtggtcag caggatttct gcatttctaa
     5341 ccactctata tctaccttga tacgggcgag taccataaaa tcctgcatta tcaagagttg
     5401 aagctccctt acccaatagc tcaccagtgt aacggggatc tgaattggta aagaaataat
     5461 attctcgtcc gattattgaa gttacatcgt aataaaaacc tatttgatca cgcattaggt
     5521 tttctgtacc gatagcaatg tttcttatct caccaattgt tgcattttgt gaaatcgcag
     5581 aaagactcgg tccgttagga tctgagaggt cattaatttc acaagctgaa aagaatactg
     5641 gcattattag taatatcagt attttactta ttttatttat ttcgaaattc ttcatcgtag
     5701 tcatttttaa aatttaacag ctaaatgaaa gttccatctc ttagaggaag gaaatggcag
     5761 cacttcaaca ccggttgaaa gacctttccc tccaaaattt gaaacttcag gatcatagct
     5821 tctatattca aagaagttta aaagattatt tccggaaaat ccaactttta cattggacag
     5881 cgggccaatc aaagatttgg gaatgttata gtataatccg atctctctca ttctagcata
     5941 ccctgcattt tcgatataag ggccactatt tgatcctaac tctcccaatc gataatctcc
     6001 attacccaaa actccggacg gatccaaatc gatcttatca tagtcagggc ttgttccacc
     6061 cagatctgtg agcaatgcgg tgaggtttat taattccccg ccctgcttcc agtgccaaag
     6121 catattaagt tcaaatcctt tgaattcaac cgtattaaga aatgacattt caaaatccgg
     6181 ctgtgcatct ccgaatacaa ccagtccgtc ttcatcagga gtagcaccta caccaataat
     6241 ttctgtgggg ctatggccct gttttattct gtattgaccc agaaaatcgg caaatcctcc
     6301 acttgtatag gaaggaacgt caagtttggt aacctcggag gtatttctcc agaaattatt
     6361 actgcttgtc cacttaatag tactggtatt aacaagattt aataacaaac ctatttcaaa
     6421 tcccttgttt tgcatttccg ccgcattggt tacaatactt gcaaaacccg aggatgtagg
     6481 cagatcggca gtcaacagga gatcagttac cgttttaaca taataagtga aatcaagcgc
     6541 aaccttgttt gacatgaaag ccgcatcaaa tccaaattca acttcagttt ggcgctcagg
     6601 ctccacctcg ctgtttccac gcgttgtacc tattacaata ccggagtttc catctataac
     6661 cgcgccggca aacgaagtaa atttatcacc aaaaacagcg aaattacctg cctgaccaaa
     6721 cgccgcacga agtttcaatt gattaactgc gttcggaaat gttacaaact catgaatatt
     6781 agctgcgagg gaagcttttg gatagtaaaa caacttattg ggatccccgt tattagatga
     6841 tttatcaccc cttacaccaa gactcacaat aattgcatct ctgaaattaa catcttcctg
     6901 cacaaaaaat ccgcggtcat gttgaataac tctgtcctga aggatagact gagagccagc
     6961 ctgatccaaa ttggtttggg aaccattcat gttagaggca gtacctcgaa ttgtatttct
     7021 gtcaaagtcg ataaaggtaa ttccaacctg cgtagtgaaa gtgaatggtg atccggcagg
     7081 gaagaaatca tgaaccagga aagcagaata attcttgtta ttactttccg tatttccttg
     7141 agcggagacc ccgcctaaac cattgccatc tttttgaaat tgtaatgtgt tcgggaacag
     7201 gacagtagtt gccatattgt aataatctat tccaccttgt aaaataagtc gtagtccatg
     7261 tgacgaagaa ttaataattc tggctgtggc ctgaccacca agtatgaatc ggtttattgt
     7321 ttcattatta gtcatcaaat ccctggtttg aagaaaatta gatgcagcat agggattatt
     7381 cggatagttt ccgtttgcat ccggctgtag ttgcgcccag ggcggtgttg ctgataaagc
     7441 aatccccatt gttgtcccgg agttatcgtt tccaaagaaa cccctgtcgg ccgaagaaag
     7501 gacataatta gtggaagcgt taagagacaa ccaatcggta actttatgat caacatttaa
     7561 tcgaaaagag ttcttctcgt acccggtatt cttgatgatc ccttcatcat ccttccggga
     7621 aaaccccaga aagtaacttg tattctcact tcctcctgaa attgtaatgc gtgaatttag
     7681 taatagacct ttgtttccaa atagctcgtc ttcgtaatca tgtaattgcc ctgaacttac
     7741 tgctgaattg aaatttgcca cttcacccgc gccaaaatgc gttgtgactt tggtggcatc
     7801 aaaagttctg actcccaacg ggttcataat ggatgtttgt cctattgcct gggataactc
     7861 aacctgagtc ttaccttgct ttcccttctt ggtggtgata atgattactc ctccggacgc
     7921 ccttgaacca taaattgccg cggctgaagc ccccttcaaa atctcaacag actcaatgtc
     7981 atcagggtta atatccgcga gacggttaga tggctgatcc tgctggagag ggttacccgc
     8041 ggcagctgcc agtgaaacaa cgttctgacc attgtttact tctgaattgt ccagataaac
     8101 tccatccaga attatcaagg gctcattgct gccattgatc gaagttagcc ctcgcattcg
     8161 gattgaataa ccaccccccg gagctccgga acttgcagtg atatttgctc ctttcagctt
     8221 tccataaaga gctccatcca gtgtgctttg gtttgtcatc ccagccaatt gatctgctga
     8281 gatcgaagca acactacttg ccaggttgct tctctttact gtagatgcca acccactaat
     8341 tacaacttct tcaagacttg taatgtcttc agacatggag acatcaactg cattggaact
     8401 acctacctgg atttcctgag acaggtagcc aataaaacta aaagcaaggg taacattttc
     8461 gttactaagg tttatccggt aattaccatc aatgtctgta atggtaccgg agcttgttcc
     8521 cttaataagt acatttaccc ccggcaagcc ttcaccggtc ttaccatcgg tgactttgcc
     8581 cgtgaccgtt ctctcttgcc caaatattgt caatggcaat aacaggcaaa gagacgtcaa
     8641 aagaataaca cttattcgtt tcatataata gttatagatt aattgaattt taaagttaac
     8701 aataattaat tggtgaaata atactatagg tgcagtttat ttaaattact tttagcctta
     8761 atcccaaaag aattactcac cttttcaata ccagcagttt atcatccatc actgttccta
     8821 caaatagata attgccgaaa ggtgcggcta cgctggatgc attaatcaga ttcccatcat
     8881 cagtaaaaat acactctact tcaggattct ccccatggag ttttattctc actacctccg
     8941 acggggcatt ttctgctttc aattgggcat acctcgtaaa tgcaatcagg ttagggtggc
     9001 agcccgaata tagcacaccg tgattatcaa attcaagatt atcaacaccg gtgccggctg
     9061 gaatatcttt caaaaaagta attgatccat cctccgatac cttgtattct ctaatcagaa
     9121 accaccggga tgaagcgaca tagagttttg cagatttaga attataagct atgccattgg
     9181 cataactaat tccatccgcc gcaagactaa atgattcacc gtcaaaatac accacactgg
     9241 atacttccag tccaagataa ctttccagaa atactccgat gcgagattta tacccatgat
     9301 cattggttaa ataaaattgg ttttcagaaa caaggactaa atcattggga tcttttagcc
     9361 attcatcttc aagagtttcc tgatggaata aagtttcatc agataataaa aaaacctcaa
     9421 tggagtgttt tccacctgca tggtttatgg caaatacttt ataatttgaa gaatcaagtt
     9481 tccatatgga aattccatgg ggattgaagg acttgtcaat gttagcagtt agcaattgag
     9541 gttgaaaaat gctggcagta agatcaaggt aatacaatcc gccaaatgca ggttctcccc
     9601 ttttgatggc catcctatcg gttgcagaaa taatcatgaa actatcttca taacttatag
     9661 caaaatcttc cgctccaaca atcggaattt cctgaaacac ctcaccatag ggtttatact
     9721 caatctccct gaaaaatccg gtgccggaaa gaatgtaata tatgtaaatg cccggcacaa
     9781 ctatgaatat gatcgacact gaaatgatct tcttcataaa atactttgtt aacaagttag
     9841 cacttgtttc aattactaac tattaaccct cccgttcatt aatttctaac ataagttgta
     9901 attttagcgc ttaattaatt ctgattaacc gtcaggcaat ggccgaatat aattttagag
     9961 aaatagaaaa aaagtggcag gcgtattggt ctgataatca aacttttgca acaaaggaag
    10021 aaaaaggcaa gccgaagtac tacgtcctgg acatgtttcc ttatccatcc ggaaaaggat
    10081 tgcatgtcgg gcatccactt ggatatatcg ctacggatat agtggcccgt tataagagaa
    10141 tcaccggatt caatgtgctg cacccaatgg gctttgattc ctttggattg cctgcggaac
    10201 agtacgcgat taaaaccggt caacatccgc aggtgacaac cgcgaataac attgaaagat
    10261 ttaaagaaca gcttagtatc cttggatttt cctatgactg gtccacggaa atcagaacca
    10321 gtgatgtgga ctactatcga tggacacaat ggatcttcct gaaacttttt aacagttatt
    10381 ttgatgaaaa ggaaaagaaa gcaaaaccga ttgcggagtt aaaggttcca ggtgaattaa
    10441 ttggcaaagc acgagatgca tatatagata atcaccggct tgcctatcag gatgagatca
    10501 tggtgaattg gtgtcctgaa cttggaactg tattagctaa tgaagaagtt gtaggtggcg
    10561 taagtgaggt cggcggatat cctgtaatca gaaaacccat gcgccaatgg atgatgcgaa
    10621 ttacctccta tgcggatcga ctgctccagg atatggaaga attggaatgg cctgaatcca
    10681 tcaagctaag tcaaaaaaac tggattggaa gatcggtagg tgcagatata tattttacgg
    10741 agaaatcaac agggaaaaag atccaggtat tcaccacccg gccggatacg atttttgggg
    10801 caacctacat agtattggca ccggagcatt ctttagttgc acaaattact actgatgacc
    10861 aaaaagagaa agttgaggga tatgtggaga aagctgcaca gaaaagcgat ctggagcggg
    10921 cagaacttga taaaaccaaa acaggagtat ttacaggttc atacgcgata aatcctgtta
    10981 ataatgaaga attacccatc tggatttcag attatgttct gatttcatat ggttacggcg
    11041 ccattatggc ggtacctgcg ggtgaccagc gggattttga atttgcaata aaatttaatt
    11101 tgccaatacg tgaggtagtg tcaaagtccg gaaaagaaag ttcggagctt atagaagctt
    11161 attctgaacc gggaataata attaactccg ggtcttacaa tggaatggca agcaccgatt
    11221 tttttgatgt tatcgtcaaa tggctggaag aaaatgatca tggaaaaggc gcggtaaatt
    11281 acaaacttcg cgactgggtg tttacacgcc aacggtattg gggtgaaccg attcccatta
    11341 tacacactga agacggccgc gtggtgggtg ttccggaaga ccagcttccg gtagttctgc
    11401 cggaggtaga atcatatacc ggaaccatgg atggactttc tccattagct aaagcagtag
    11461 attgggtaaa cactattaat cctgaagatg gccggcccgc aaaaagggaa accaatacca
    11521 tgccccaatg ggcaggttcc tgctggtatt atctcagata tctggataat aagaatgaga
    11581 acgaatttgt tccaaaggaa aaagaaaatt attggctccc ggtagacctc tatgttggag
    11641 gtgctgaaca tgctgtattg catttgcttt attcacgctt ctggcataaa gttctatttg
    11701 acctgggtta tattaattcc aaagaaccat tccggaagtt gatcaaccag ggcatgatca
    11761 gtggcaaatc gaacttcgtg taccggattc agggttccaa taaatatgtc tccctgggat
    11821 tgaaagatca atttgaatca gatcccatac atgtggatgt aaatatcgta aacgatgata
    11881 ttcttgacct ggaggcattt aaggcttatt caagtctgaa taatgatgct gaatttgtgt
    11941 tggaagacgg taagtatgtc tgcggattcg cgatcgagaa aatgtctaaa tctctattga
    12001 atgtggtgaa tcccgatact cttgtagaaa aatacggtgc ggatactttc cgaatgtatg
    12061 aaatgttttt agggccgctt gagcaggcaa aaccctggaa taccaatggc attgatgggg
    12121 tttggaaatt cctgaagaga ttttggaatt tatatcatga tgaatccggt aagctcagtt
    12181 tatcagggga agatccttcg caggaggagc tcaaaattct acataagacc atcaaaaaaa
    12241 taaaacagga tattgaaagg tattcattta acacagccgt tagcgccttt atgatttgtg
    12301 tgaatgaact tacaccgctt aaatgcaata aagaaaaaat attacaaccc ttattgatta
    12361 ccctctcccc ttttgcgcct cacctcgcgg aagagatttg ggctgaaatg ggtaatacta
    12421 cctctattag ctttaccagg tttcccgatt atgatgaaac ccatatccag gaagattcat
    12481 ttgaatatcc cattgccatt aatggtaaga tgcgggtaaa aatgaaattc gctttggata
    12541 tgccgtctga agatattgtg aaaatggttt tggcctcaga gatagttaaa aagtggactg
    12601 atggaaagac gcccagaaag gtgattattg ttccaaaaaa aattgtgaat atagtcgttt
    12661 gagctactct ggttccccac cgatgttctg cttaggatcc tgctgatttg gttcagtgcc
    12721 ttcttcagat tgactttctt ttttcttcct tttgaaaagt ccttttagaa atcccttttt
    12781 ctccccggaa ccttcaacgc cctccgtttc aattgcggaa gtatcgggtt ccaattccgc
    12841 cgccccagtg gaaacaggtt cgatgggtat tttcaatttc ttaataagat aattaaaata
    12901 attttcctga tcaatgttga aatgccaatt cctggccacc tcggtagtat caggtttgaa
    12961 agcagaactg tcgggaatat ccaactccgc agccatcaat tcatcaccca aaaattccag
    13021 cgaatctgga agttccggat aaataactac cggctcaata gtatttattt tggcaaaagg
    13081 ggaatgaatc ggcggtggaa ttttattaat caccgtggca ataaatggct cctgcccgat
    13141 accgaatttg tttttataca ctttggcaat atctgtcctc ggaatgctgt caacgaaata
    13201 gctaaagtga tctttttgca ctttttcatc taacaaataa aaactttcga aggcggggca
    13261 taccttttga gaacaaccct gaaaaaggca taaaataaga cctagagcca aaaaaggaat
    13321 gcggcttttc aatttcagtt gggcaaaata tatatgtata ttaaccttaa aagtagccgg
    13381 attatttcac tatttaaaga aattctaaat taatttgggg aaaatcagct cttcctaaga
    13441 ctttgaagtt ggattttgac tgattttggc agctttttaa ctaccaaatt atagctatct
    13501 cttatccagc tgattaatag ctttttagat acagcacctt taattgtcac tgtattccag
    13561 tgtttcttgt tcatatgaaa gcctggctaa acttcttcat acttttcccg gagctcaatc
    13621 gctttttccg gatcacattt caaatttata ctttcaaaaa acccaagatt tgttatagcg
    13681 aatattctac cttcaacttt atataccaga gtttcttcat caaaaggaaa ctcctcagtt
    13741 acacctggca gagaaaggca aaatgaccgg aattcttcaa tattcatccc attaaaaatc
    13801 tcctgattag gaaaataatc aatgcgccaa gaaaaaccag aatggaaatc ctattgatgc
    13861 catgcatcac tttaatgttg aaattggtag
//
LOCUS       DASWHX010000147        19485 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_1636675, whole genome shotgun sequence.
ACCESSION   DASWHX010000147 DASWHX010000000
VERSION     DASWHX010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 19485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19485
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1636675"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..300
                     /locus_tag="VGA21_09940"
     CDS             <1..300
                     /locus_tag="VGA21_09940"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX9650870.1"
                     /translation="GFNAWSGNLLWEEKSAGRPSELKILNGIVYFTTGGDGRLHALDI
                     QTGKHIWKLKCPDKNYDPSYPFKSDGVAVFQGKNGKKGKIIASGFINAYCYEAIR"
     gene            284..1075
                     /locus_tag="VGA21_09945"
     CDS             284..1075
                     /locus_tag="VGA21_09945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650871.1"
                     /translation="MRRSGKKFPNVLGVTFLLSELVSCVPRYGPALLGSDLPYMNMPV
                     FEDRTAAEVYISASATNSMLDLGKNGNNNSYQLSIHNGLAFKNFNTGMGIYAYAGGAR
                     NLNVSRNILYNPTGFTDDKNFYGAGVRGQINYAKTLNKFIIRGGAKASVTAEYGDYFQ
                     FRRVAQAMEGIINMRKKNYFISYAIFGELKIPFENKEKEHTGHIGIYAGVIYSTMDTP
                     RFSQSYPSIGIVCDYKRFHFLVSAQLTILDDAFLAATITYSVIKK"
     gene            1147..1914
                     /locus_tag="VGA21_09950"
     CDS             1147..1914
                     /locus_tag="VGA21_09950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009583292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent peptidase"
                     /protein_id="HEX9650872.1"
                     /translation="MIAGFAAIAIVLLAIWIKIRYLDYRFYTPIILPLKSKFKKPLQQ
                     YFPYYQGLNEKEKRDFERRVQYFIDVKEFYPRNMEAVTDEMKALISASAIQLTFGLPN
                     IFLAHFKRILIYPDTFFSTTNQQMHKGEVNPGHRIIVVSWNNFVSGYIELDGINLGLH
                     EMAHALHLENRIMNNEYGFFDEAYIEKWNQLSGLEIEKIRKNKNHFFREYAGNDMEEF
                     FAVAVEYFFETPKAFKENLPELYDSLSKLLNQDPASL"
     gene            1911..3266
                     /locus_tag="VGA21_09955"
     CDS             1911..3266
                     /locus_tag="VGA21_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009580500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HEX9650873.1"
                     /translation="MMNWVQLLSPARSIEEVQNTSASDNLARTVFEQDFDRIIFSHPF
                     RRLQDKTQVFPLPDKDFVHTRLTHSLEVSSVGRSLGRKAGVAICDRHGELRQQGYSEF
                     DLGAITAAASLAHDLGNPPFGHSGEDAISEFFTTHNTGLSFKDKVSGEEWSDLIKFEG
                     NAQGFRIITRKELGLKLTFATLGAFTKYPRPSLISKTEESRKSQRKFGFYSSEVNKFE
                     NIAKTLGLIRSDAQIWRRHPLAFLVEAADDICYQIIDLEDGCRLGLVSYENTEELLAK
                     ILGDKIDKAKLNRKATIEEKVGTLRALTIGTLIDQCSECFLDNESKILEGDFDTALTK
                     QIPSASALETIADLSLEKIYKSQVVIEIEAAGFEVLPGLLDAFTSAAWSQNHHQNFTQ
                     KHKAILRLLPGDITAALSNSGKDTYTILRNILDFVSGLTDKHALNLFRQIKGISLPKF
                     G"
     gene            complement(3322..3774)
                     /gene="tnpA"
                     /locus_tag="VGA21_09960"
     CDS             complement(3322..3774)
                     /gene="tnpA"
                     /locus_tag="VGA21_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015027793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HEX9650874.1"
                     /translation="MAGTFTQLYIQIVFAVKGREKLIAKSWKQQLNEYISGIIRNKNQ
                     KAIIVNGMADHIHVFIGLKPSISISDLVRDIKNNSSNFINKSKLVPGKFSWQEGYGAF
                     SYSHSHISKVYNYILNQEKHHRKKTFREEYIDLMMKFNIPFDEKYLFD"
     gene            4143..6581
                     /locus_tag="VGA21_09965"
     CDS             4143..6581
                     /locus_tag="VGA21_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017730328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX9650875.1"
                     /translation="MWTQLSHTIIKYRLIFVIILALITAFMGYKARDIEWSYDLAKVV
                     PEDDRDMQVFSDFKELFGEDGNLVAIGLKDSAIYEVENFNRFRMLSEEVAKLAGIKEV
                     VSLPLIMRLQRDEGIKEFRLEPIFPEYINTQPELDSLLAVALDQKFYSGQLINEDNGA
                     VLLLIGIDKDVLNTEYRIQLTQDIISIGDIFSNRTGIELHYAGLPFVRSVIAGGVKKE
                     MLMFIIFSVVITGLIMLMFFRSFYAVIFPLTIIGVVVVWVLGTLVLFGYKITILAGLI
                     PPVIVVIGIPNSIYLLNKYHQEFLKHGNKVMAISRVTRKIGFTTLMTNFTTAIGFLVL
                     GFTDIIILKEFGIVAGINIMATFLVSIILIPSVFSWLPPPSRRQMRHLEFKPLGAALH
                     SLDLLVHRNKYRIFLVTSVIVVISAIGINKVHSISYMVDDIPAESQVRKDLTFFENNF
                     SGIMPLEIVVDLGQRRGITNIRNLQKLEEFERFLDSQEDISKPVSIVSFVKAARQAYY
                     NNNPSYYDLPNNRDRAFILRYFRNQGDSSGILKSFVDTTGQIVRISMKIADIGSTRMD
                     SLVNQVIEPEKERIFAGTEITAKVTGTTPLFIKGNKFLIENLKFSFLLAFVLISLIMA
                     ALFLNWKMITISLIPNIIPLMITAAIMGYFNIPLKPSTAIVFSITFGISVDYSIHFLA
                     KYRQELFANKFFVPVAVSKSLMETGTSMIYTSVVLFAGFVIFVASDFGGTVALGILTS
                     TTLFIAMIANLVVLPSLLLAFDSGKRRKDSHPLIEQYEEFYNEDEDEEIDLGRISIEV
                     NGMDSEAAKKEDKV"
     gene            6593..9922
                     /gene="ileS"
                     /locus_tag="VGA21_09970"
     CDS             6593..9922
                     /gene="ileS"
                     /locus_tag="VGA21_09970"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009194058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEX9650876.1"
                     /translation="MSKSQKYQEYKSVNYVQLAREVLKYWHDHDIFKKSVANRNGAKE
                     FTFYEGPPSANGTPGIHHVMSRTVKDLFCRYKTLKGFQVKRKGGWDTHGLPVELQVEK
                     HLGITKEDIGVKISIEEYNRKCKEAVMKYKDDWDDLTERIGYWVDLDNPYITYETNYV
                     ESLWYLLKELYNRDLLYKGYTIQPYSPAAGTGLSSHELNQPGTYKDISDTSVVAQFKI
                     SGTENEYFLAWTTTPWTLPANSALAIGENLKYVKVETFNAYTYKPVKVILAKDRLSAY
                     FKKEAESVELNTYNPGSKHIPWKVINEYSGKDLVGMDYDQLMPYVPLPKPAFTVVPGD
                     FVTTEEGTGIVHISKTFGADDYRTCVKFGIPGVLVKDEEGNEQPIVDKKGRFVKEITD
                     FAGMFVKNYSDENESSAEYRSTDVKIAIKLKEENKAFLVEKYLHSYPHCWRTDKPILY
                     YPLDSWFIKTTACKDKLVELNNTINWKPESTGKGRFGNWLENLVDWNLSRSRYWGTPL
                     PIWVTRNKEEQKCIGSIKELQTEIQKSIEAGLMKELPWEKGEIDLHRPYVDQIILVSE
                     KGEPMYREPDLIDVWFDSGAMPYAQWHYPFENKEIFEKSFPADFIAEGVDQTRGWFFT
                     LHAIAGMLFESVAFKNVISHGLVLDKDGNKMSKRLGNTTDPFKTMDTYGPDSIRWYMI
                     SNANPWDNLKFNEEAVSEIQRKFFSTLQNTYSFFALYANLDKFNYTDGEINHDSLTES
                     DRWILSRKNSLIKRVNESFEDYEPTKAVRAIQNFVVNDLSNWYVRLNRKRFWKGEYNE
                     DKKAAYQTLYECLVVVAKLGSPVAPFYMDRLYMDLNKVTGRESAESVHLSDFPSVNDS
                     LMEMELEQRMELAQGISSLVHSIRKISKIKVRQPLSKILIPIQGAKFKNQIEAVRDLI
                     LNEVNVKEIEYIDDASDVLVKKAKPNFRKLGKEYGPQLKELSEAISNFTAEEIKKIEI
                     GETVGVNTSKGKVNLTLDDIEVFSEDIPGWSVASEGKITVALDVTVSDDLKMEGIARE
                     LVNRIQNIRKDKGMEVQDKISIQIEKVSELISRSILKFENYICTETQALSMKLVEELA
                     DGEILEMDEANVKVLVSKVTT"
     gene            9919..10596
                     /locus_tag="VGA21_09975"
     CDS             9919..10596
                     /locus_tag="VGA21_09975"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008628114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein signal peptidase"
                     /protein_id="HEX9650877.1"
                     /translation="MIEKDNIFKYFLLTLAIIFLDQVVKVSVHTYMDLGTIGQIKVFG
                     EWFKLHYTLNPGMAFGMQLGSPYGKLFLTIFRLFAMVGIAYYLYYLARKKVHPGLLWC
                     IALILGGAVGNVIDSTFYGVIFNNAPYDASTPWFHGQVVDMFYIDIWEGQVADWIPLW
                     GGQYIALWPIFNIADASIFCGVTLILIMQKKFFVEHEKETKVAEAVNEMGESKATQLP
                     DESKSID"
     gene            complement(10593..10895)
                     /locus_tag="VGA21_09980"
     CDS             complement(10593..10895)
                     /locus_tag="VGA21_09980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009195293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HEX9650878.1"
                     /translation="MRKKSSAYSENTIKNAFEDLLETYQIKDKYSEAALISSWERLLG
                     KTIAKRTEKLFIKNRILYAELNSAALKHELNSSKEKILEIFEKEFGRKVITDLVLL"
     gene            complement(10915..12018)
                     /gene="recF"
                     /locus_tag="VGA21_09985"
     CDS             complement(10915..12018)
                     /gene="recF"
                     /locus_tag="VGA21_09985"
                     /inference="COORDINATES: protein motif:HMM:TIGR00611.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HEX9650879.1"
                     /translation="MHLESLSLHNFKNFREITVQFEDRITCILGENGSGKTNLLDAIY
                     YLSLGKSAFNPQDNQNINHGELFFSILGNLRTNGDFASIQCSLKKGNKKRLRVNGVDY
                     PKLSEHVGKYPLVLIAPDDSDLVREHSEARRKFIDGILCQFSQDYLSDLMKYNNILKQ
                     RNAALKSLSEKKKPDKTLFEVYNKSLFDLNRVIHTFRSNFIENFKPYLNSYYSDISED
                     KEEISLSYKSSVKARDFEKVFRNNFERDLILQRTELGIHRDDFIFKINSHGLKKFGSQ
                     GQQKSFIISLKLAHYQFLKEEKKILPLLLLDDIFDKLDDRRISRFMKVMKSPDFGQVF
                     MTDARQKRSRDIIKKNKIKAKFICIDNNIIKAC"
     gene            12098..13135
                     /gene="pdhA"
                     /locus_tag="VGA21_09990"
     CDS             12098..13135
                     /gene="pdhA"
                     /locus_tag="VGA21_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009195290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HEX9650880.1"
                     /translation="MGKTKEAKESKSTRKQKASFDDKTYMFWYESMLLMRRFEEKCGQ
                     LYGQQKIKGFCHLYIGQEAGASGAISALTRDDKWITAYRDHAHPLGLGTDPGKVMAEL
                     FARETGVSRGKGGSMHMFDVERNFYGGHGIVGAQIPLGTGLAFAEKYKGNKNLCICYM
                     GDGAVRQGAFHEALNMAMHYRLPVIFVIENNGYAMGTSVKRTSNVHDLYTLGEAYDMP
                     SRSVDGMSCEEVHEAVSDGAERARNGNGPTLLEFRTYRYKGHSMSDPAKYRTKEELEE
                     YKAKDPIERVKNTILKNKIATEKELEKIEEKIKQQVQDAVNFAESSPFPPPEQAFEDI
                     YMQKDYPFIKD"
     gene            13192..13896
                     /locus_tag="VGA21_09995"
     CDS             13192..13896
                     /locus_tag="VGA21_09995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017730316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9650881.1"
                     /translation="MAKKNKKGNKQGGVLESSEVLQEEFTRFEHYLETHKSLVFVIVG
                     VVLVAIISILGYRYYISQQDELAQKEMFQAVYYFESDSLDLALNGDGNHLGLLDIIDE
                     YGGTDAANLAEYYAGTAYLLKGNYNTAIEYLGSFSSGDILIQGRAYSLTGDAYMELGN
                     YGKAAEFYNKAANYKPNQHFTPQYLMKAALAYEKLNDYPTAKEMYDRIVEEFFNSSEF
                     QDAKKQSARMQALISS"
     gene            13973..14449
                     /gene="ribH"
                     /locus_tag="VGA21_10000"
     CDS             13973..14449
                     /gene="ribH"
                     /locus_tag="VGA21_10000"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010609755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEX9650882.1"
                     /translation="MASAQKNLSSHSRRNIFDISKTKFGIVISEWNDDVTDSLYSGCM
                     QTLLDKGAKRGNIITHYVPGSFELPLGAKWLATEKKIDAVICLGAVIQGETRHFDFIC
                     SAVAQGIMNVGLEFSKPVVFGVLTTNTKKQALDRAGGKLGNKGDEAAITAIKMLGF"
     gene            complement(14446..15948)
                     /locus_tag="VGA21_10005"
     CDS             complement(14446..15948)
                     /locus_tag="VGA21_10005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009579157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HEX9650883.1"
                     /translation="MRSVITALFWGIIILGGCKSKEYTSLLTHSYASAGISVEENSFP
                     SSETSDDSMEDHPTAPAWLVTPFVLLLLLIATGPLFYEHFWHKNYPAISIILAVIVVA
                     YYLFVLHNIHDPVHHLFEYIQFIALLGSLYIASGGIMIEVDKEARPATNVFILLVGAV
                     IANVIGTTGASMLLIRPFIRLNKYRIKPYHIVFFIFLVSNIGGALTPIGDPPIFLGYL
                     KGVPFFWTLEHNVLAWAMATTIFLLIFYYFDKKNSSYYNLDDEPPKYTGKLAIKGSRN
                     FIWLMVIVGAVFIDPNVFDWVPSIEIQGNSLSFVREIIMITVGILSYKLANKNALKGN
                     EFTFEPIKEVAFIFIGIFGTMLPALELVGHFAASPEGVKLINHDTLYWGTGILSGFLD
                     NAPTYINFLTAAMASEGASINNHADVTAYAAGGVYYDSILYLKAISIAAVFFGAVTYI
                     GNGPNFMVKSIAEQVGIKMPSFFGYIIRFSIPILLPVLFLTWILFFVLAS"
     gene            17167..17499
                     /locus_tag="VGA21_10010"
     CDS             17167..17499
                     /locus_tag="VGA21_10010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650884.1"
                     /translation="MENLLLFHLMIGPVVLGLSFLYKYYPNIQAVFWKQKIDNKEQTI
                     WREAVRYSANAMIIGSFFTIIFQTISVLTMQPSSSIWASSIFLGSCLLGILPLTEIHL
                     RKKYWYKD"
     gene            17573..18910
                     /gene="trkA"
                     /locus_tag="VGA21_10015"
     CDS             17573..18910
                     /gene="trkA"
                     /locus_tag="VGA21_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009580056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HEX9650885.1"
                     /translation="MKVIIAGAGDVGFHLAKLLAYEEHDIVLIDKDSEKLKAASNSLD
                     VYTIKGNATYFDVLAEANVEEADLFIAVTQSEETNLTTSIIAKKLGAKKTVARIQNTE
                     FLFRKDVLDLRDLGIDEIISPESLAAREINRLLKETALTDTFVFEQGLLSLIGVIVDE
                     NSELTKKSLSEISESQDHEFINVAILRDNETKIPRGMSRLLPNDHAYFVTIPSGVDKV
                     VSLAGKQKINIKNIMILGGSRVGYHAAKKLSKKFKVKLIENDKDKAFELADMLPDVMV
                     INGDGRDVELLLEEGIENIDAFIAVTGNSETNIISCLVAKSNGVKKTISLVENIDYIH
                     LSQNIGVDTMINKKLIAANFIFRHIRRGDVISLASIHGVDAEILEFMVNEDSKIANKV
                     IKEIDFPANAIIGGVIRNNEGHITMGDSVLLPDDRVVVLCKPDCIRKVEEFFK"
     gene            18907..>19485
                     /locus_tag="VGA21_10020"
     CDS             18907..>19485
                     /locus_tag="VGA21_10020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020529458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HEX9650886.1"
                     /translation="MRPHLKFIINFLGLLLVLNGIFMLSAVPFAWYYNEPITPIVYSA
                     LISIIVGLPAWLAIKIEKGRDIRKRDGYLIVTLGWLIMSFSGTLPFILSGAIPNFTDA
                     FFETISGYTTTGSSILNDIESLPKSILLWRSLTQWIGGMGIIVLTIAILPILGIGGMQ
                     LFMAEAPGITADKLKPRIQDTATRLWLIYVMLT"
BASE COUNT         6121 a         3648 c         4080 g         5636 t
ORIGIN      
        1 ggcttcaatg cctggtccgg caatttgtta tgggaagaaa aatcggcggg gcgtccatct
       61 gaacttaaaa ttctgaatgg catcgtctat ttcacaaccg gcggcgacgg ccgcctgcac
      121 gccttggata ttcaaactgg caagcacatc tggaagctga aatgccccga caagaattac
      181 gatccgtcgt acccgttcaa atccgacggc gtggcagtat tccagggcaa gaacggcaaa
      241 aaaggcaaaa tcatcgccag tggttttata aacgcgtatt gctatgaggc gatcaggtaa
      301 gaaatttccc aatgtattag gagttacctt cctgctatca gaattggttt cttgtgttcc
      361 caggtatggg ccggcgctac ttggttcgga tcttccatat atgaatatgc cggtttttga
      421 agacaggacg gccgccgaag tttacatttc agctagcgca accaactcca tgctggatct
      481 gggaaaaaat ggaaacaata attcttacca gttatcaatt cataacggtc tggcatttaa
      541 aaactttaat acaggcatgg gaatttatgc gtacgcagga ggagcacgca atctgaatgt
      601 ctcaagaaat attctctaca acccgactgg atttaccgat gataagaact tttatggagc
      661 cggtgtgcga ggtcagatta actatgccaa aacgttgaac aaatttataa tacggggagg
      721 agccaaagcc tccgttactg cagaatatgg agattacttt caattcagaa gagtagccca
      781 ggcgatggaa ggaattatta acatgcgaaa gaaaaattac tttatttcct atgcaatttt
      841 tggtgaacta aaaattcctt ttgaaaataa agagaaggaa cacaccggcc atatcggcat
      901 ttatgcaggt gttatctatt ccacaatgga caccccgcgt ttcagtcagt catatccttc
      961 aatcggcatc gtttgtgatt acaaaagatt tcattttttg gtgtcagctc agttaacgat
     1021 tttggatgac gcttttcttg cagcaaccat tacctattct gttatcaaaa aataaaggaa
     1081 gggcgttacg ccaaagaagt taaatattgg caatttctct ctactttaga gaccaaacca
     1141 cgcatcatga ttgccggctt tgctgccatt gccattgttc ttcttgccat ctggattaaa
     1201 atccgctact tagattaccg cttttacact ccaataattc ttccgctaaa atcaaagttc
     1261 aaaaaacctc tgcaacaata ttttccctac taccaggggt taaatgaaaa agagaaaaga
     1321 gattttgaac ggcgggttca gtatttcatt gatgtgaaag aattttatcc aagaaacatg
     1381 gaagcggtta ctgatgaaat gaaagcgtta atttctgctt cagcaattca gctgactttc
     1441 ggattgccaa atatttttct agcccacttc aaaagaatcc tgatttaccc ggatacattt
     1501 ttttccacca ccaatcagca aatgcacaaa ggcgaagtga atcctggtca ccgtataatc
     1561 gtcgtttcct ggaataactt tgtttccggg tacatcgaac tggatggaat taacctcgga
     1621 ttgcatgaga tggcacatgc tctgcatttg gaaaacagga ttatgaataa tgaatatggc
     1681 ttttttgacg aagcttacat agagaagtgg aaccaattat ccgggctgga aattgagaaa
     1741 atcaggaaaa ataaaaatca tttcttccgg gaatacgcgg gcaacgacat ggaagaattt
     1801 tttgctgtag ccgtggagta ttttttcgaa acccctaagg cgtttaaaga aaatttgcct
     1861 gaactttatg attcactttc aaaattatta aatcaggatc ctgcaagctt atgatgaatt
     1921 gggttcaact tctttctccg gcgcggtcaa ttgaagaggt gcaaaatact tcagcctcag
     1981 ataacctggc ccgtactgta ttcgagcagg atttcgaccg tattattttt tcacacccct
     2041 tccgcagatt gcaagacaag acccaggtat ttccgttacc ggataaagat tttgttcaca
     2101 cccggttaac tcacagcctg gaagtttcaa gtgtcggaag gtcgctgggt aggaaagcag
     2161 gggttgcaat ttgcgaccgt cacggggaat tgcggcaaca agggtattca gagtttgatc
     2221 tcggtgccat aaccgccgca gcatcgcttg cccatgatct ggggaatcca ccgtttggac
     2281 attcggggga agatgccatt tcagagtttt ttacaaccca caataccggg ttatctttta
     2341 aagacaaagt ttccggtgag gagtggtcag accttatcaa gtttgaaggt aatgcccagg
     2401 gttttcgtat aattactcgc aaagaacttg gattgaaatt aacattcgct acgcttggcg
     2461 catttaccaa atacccaaga ccttcattga tctcaaaaac cgaggaaagt agaaaaagcc
     2521 agaggaaatt tggattttat tcttcggaag tgaataagtt tgaaaacatt gccaaaacac
     2581 ttggattaat aagaagtgat gctcaaatct ggagaaggca cccgcttgct ttcctggtgg
     2641 aagcagcaga tgatatttgc tatcagatta ttgatttgga agatggctgt agactgggtt
     2701 tggtaagcta tgagaataca gaagaattgc tggccaaaat tcttggagat aaaatcgata
     2761 aggctaaact taatcgcaag gcaaccattg aagagaaagt aggtacgttg cgggcattga
     2821 caattggaac actgattgat cagtgctccg aatgttttct tgataatgaa tctaaaattc
     2881 ttgaaggcga ttttgatacg gcattgacaa agcagatacc ctccgcgagc gccctggaaa
     2941 caatcgctga tttgtctcta gaaaaaatct acaagtcaca ggtggttata gaaattgaag
     3001 cggcgggctt cgaggttttg cccggcttgc tggatgcgtt taccagcgcg gcctggtcgc
     3061 aaaatcatca tcagaatttt acccaaaaac acaaagcaat tctaagatta ttgcccggcg
     3121 atatcactgc cgcgctaagc aattccggga aagatactta taccattctg cggaatattc
     3181 tggactttgt gagcggcctc accgacaagc acgcgttgaa tcttttcagg caaataaaag
     3241 gaatctcgct gcctaaattc gggtagttga tagatatagg agaatcagaa tgaagatatt
     3301 cgaccttcag tttttaccat tctaatcaaa taaatatttc tcatcaaaag gtatgttaaa
     3361 tttcatcatc aaatcaatgt attcttcccg aaaagttttt ttccggtgat gcttctcctg
     3421 attcaaaata tagttgtaca ctttgcttat gtgagaatgc gagtaggaga aagctccgta
     3481 gccttcctgc cacgaaaatt ttccaggcac cagtttactc ttattgataa agttggagga
     3541 attattctta atgtccctaa ccaaatcgga aattgaaatt gagggtttca aaccaataaa
     3601 cacatggatg tggtcagcca ttccattaac gataatagct ttctgatttt tatttctgat
     3661 gattcctgaa atatactcat tcaactgttg cttccaggat tttgcaatta gtttttccct
     3721 acctttaact gcaaaaacaa tttgaatata caattgcgta aatgtgccag ccataactat
     3781 caccccttcg gggtttgtag gtttttgatt tatataatta tagtattacc atcccttcgg
     3841 gatttcagaa tgcaaaaata aataaaggat caagaaaatc ccgaagggat ggaagtatta
     3901 taggccaaga aattccgaag ggatgaaaga attataggat gaaagtatta tagggatgaa
     3961 agaattatag gatgaaagaa ttatagggat gaaagtatta caaggatgaa agcattatag
     4021 gccaagaaat cccgaagaat taaagtatta tagccaaaaa aatcccaaag gggcgaaagc
     4081 agcgattccc tattactcta aaatcattac ctttgtggtc ttattttcga ccctatccct
     4141 tgatgtggac ccaattatct catactataa ttaagtacag gcttatcttt gtgataatcc
     4201 tcgcacttat tactgcattt atgggatata aagccaggga cattgaatgg agctacgatt
     4261 tggccaaagt tgttccggaa gatgaccggg atatgcaagt cttttcagac tttaaagaac
     4321 tttttggtga agacgggaac ctggtagcga taggtttgaa agacagcgcc atttacgaag
     4381 ttgagaattt taaccggttc cggatgcttt ctgaagaagt tgcaaaactt gccggaatca
     4441 aagaagtggt atcattacca ctaatcatgc gattgcagcg agacgaggga ataaaagaat
     4501 tcaggttgga acccatcttt cctgaatata ttaatactca gccagagctc gacagcttgc
     4561 ttgctgttgc attggatcaa aaattttatt ccggtcagct tatcaatgaa gacaacgggg
     4621 cagttctttt acttatcgga attgataagg atgttctgaa taccgaatac agaatccaat
     4681 tgacacagga tattattagt atcggtgata tcttcagcaa cagaacaggt atagaattgc
     4741 attatgccgg gcttccattt gtgagatcgg taattgccgg cggcgtaaaa aaagagatgc
     4801 tcatgttcat catcttttcc gtagtaatta ccggattgat tatgctcatg ttcttccgct
     4861 cattttacgc agtcattttt ccgctaacaa ttattggagt tgttgtggtc tgggtgctgg
     4921 gaaccctggt tttgtttggg tataaaatta ccatactcgc aggtctgata cctcccgtaa
     4981 ttgtggttat aggtattcct aacagtatct atcttttgaa taagtatcac caggaattcc
     5041 ttaaacacgg aaacaaagtg atggccataa gcagggtaac ccgcaaaatt ggttttacaa
     5101 cactgatgac taattttact acagccattg gattcctggt cctgggattt acagatatta
     5161 ttatcctaaa agaatttgga attgtggctg gaatcaatat catggctaca ttcctggttt
     5221 ccattattct gattcccagt gttttttcat ggctcccacc tccttccagg aggcaaatgc
     5281 gccacttgga gtttaaaccg ttaggagcgg ctctgcacag tctggatttg ctggttcacc
     5341 gcaataaata ccggatattt ctggttacat cagttatcgt ggtgatctcg gcaatcggaa
     5401 ttaacaaggt acactcaatt tcgtatatgg tcgatgatat tcctgctgag agccaggtaa
     5461 gaaaagactt aacttttttt gaaaataact tcagtggcat catgcctctt gaaatagtcg
     5521 tcgaccttgg acagcggcgt ggaatcacaa atatcaggaa tcttcaaaaa ctggaggaat
     5581 ttgaaagatt tcttgattcg caggaagata tttcaaagcc agtttcaatt gtgagctttg
     5641 tgaaagctgc acgccaggcc tattataaca acaatccttc ctattacgat ttgccgaata
     5701 acagggatag ggcatttata ctccggtact tcagaaatca aggcgatagt tctggaatat
     5761 taaagtcttt cgttgataca accggccaga tagttcgaat ttctatgaag atagccgata
     5821 taggttcaac caggatggac tcgctggtaa accaggttat agaaccggaa aaagaaagaa
     5881 tatttgccgg cacggaaatt acggccaaag ttaccggcac cacaccctta tttattaaag
     5941 gaaataaatt cctgattgaa aatttgaaat ttagcttcct gcttgcattt gtgcttattt
     6001 cattaataat ggcggctctg tttctgaatt ggaaaatgat tactatttca cttattccca
     6061 atattattcc actcatgata accgcggcaa tcatgggcta tttcaatatt ccgcttaagc
     6121 cgagtacggc tattgtattt agtattactt tcggaatttc agttgattat tccattcact
     6181 ttctggctaa atacaggcaa gagttatttg ccaacaaatt ttttgttcct gttgccgtaa
     6241 gtaaaagcct gatggagaca gggacgtcga tgatttatac ttcggtagta ttatttgcag
     6301 gctttgtcat ttttgtggca tctgattttg gcggaacggt agccttggga atcttaacct
     6361 caaccacgct gtttattgcc atgattgcta atctcgttgt tttgccgagt ttacttcttg
     6421 ctttcgacag cggtaaacgc aggaaagatt cccatccatt aatcgagcag tacgaagaat
     6481 tttataatga agatgaagat gaagaaattg atctgggtag aatatcaatt gaagtaaatg
     6541 gaatggattc cgaggctgct aaaaaagaag ataaggtttg aatttacctg ccatgtctaa
     6601 atcacaaaaa tatcaggaat ataaatcggt gaattacgtg caacttgccc gggaagtgct
     6661 caaatactgg catgaccatg atattttcaa aaaatctgtt gcaaaccgga atggtgcaaa
     6721 ggaatttact ttctacgaag ggcctccttc agcaaacggt acgccgggaa ttcatcatgt
     6781 tatgtcacga actgtaaaag atcttttttg ccgctacaaa accttaaaag gatttcaggt
     6841 gaaaagaaaa ggtggttggg atactcacgg ccttcccgtc gagttgcagg ttgaaaagca
     6901 cttgggcata acaaaggaag atatcggagt taagatttct attgaagaat ataaccggaa
     6961 gtgtaaggaa gcagtgatga agtataaaga tgactgggat gaccttaccg aaagaattgg
     7021 gtattgggtt gatctagata acccctatat cacctatgaa accaattatg tagaatcact
     7081 ctggtatctt ttaaaggagt tatataaccg ggatttgctc tacaagggct atactattca
     7141 gccttattct cctgcagccg gaaccggcct tagctcacat gaattaaacc aaccgggtac
     7201 ttacaaagac atctctgata cttccgttgt agctcagttc aaaatctccg gcaccgaaaa
     7261 tgagtatttc ctcgcctgga ccaccacccc ctggacactt ccggctaaca gcgcgctagc
     7321 cataggagaa aaccttaagt atgtgaaggt cgagacattc aatgcctaca cctacaaacc
     7381 ggtcaaagtc attcttgcaa aagatcgctt atccgcgtac ttcaagaaag aggcagaaag
     7441 tgtagaactg aatacatata atcccggaag taaacatatc ccatggaaag ttattaacga
     7501 gtattcaggc aaagaccttg ttggaatgga ctatgaccaa ctgatgcctt atgtgccgct
     7561 tccaaaacct gcttttaccg ttgttccagg ggattttgtt acaaccgaag agggaaccgg
     7621 aattgttcac atctcaaaaa cttttggtgc tgatgattat agaacctgcg tgaaatttgg
     7681 aattcccgga gtattggtga aggatgaaga gggcaatgaa caaccaattg tggacaaaaa
     7741 aggccggttc gtaaaagaaa tcacagactt tgccggaatg tttgttaaga attattcaga
     7801 tgaaaatgaa tcttcagcgg aataccgatc gacagatgtt aaaattgcaa tcaaattaaa
     7861 agaagaaaat aaagcctttc tggtagaaaa gtatcttcac tcttatccac attgctggcg
     7921 aacagacaag cctattttgt attaccccct tgattcgtgg ttcattaaga ctacggcatg
     7981 caaggataaa ctggttgaat taaataatac gataaactgg aaacctgaaa gcaccggaaa
     8041 aggaagattt gggaattggc tggaaaattt ggttgactgg aacttaagcc gttcccgcta
     8101 ttggggaaca ccattaccca tttgggttac caggaataaa gaagaacaaa aatgtatagg
     8161 ttcaataaaa gaactccaaa ctgaaattca gaaatctatt gaagccggct taatgaaaga
     8221 acttccctgg gaaaaaggag aaatcgattt acatcggccc tatgtggatc aaataatttt
     8281 agtaagtgaa aaaggcgaac ctatgtatcg tgaaccggac ttgatcgatg tatggtttga
     8341 ttccggtgct atgccttatg ctcaatggca ctatccattt gaaaataagg aaatctttga
     8401 gaaaagtttt ccggcagatt ttatcgcgga aggcgtcgat caaacccgcg gatggttctt
     8461 cactttgcat gccatcgccg gaatgctttt tgaaagcgtt gctttcaaaa atgtaatttc
     8521 ccacggactt gtcctcgata aggatggaaa caaaatgtca aaacggctgg gtaatactac
     8581 ggatccattc aaaaccatgg atacttatgg tcctgattct attcgctggt acatgattag
     8641 taatgctaac ccctgggata atcttaaatt caacgaagaa gcagtatcgg aaattcaacg
     8701 caagtttttc agcacacttc aaaatactta ttctttcttt gccttgtacg caaatctgga
     8761 taaattcaat tacactgatg gtgaaatcaa tcatgattca ttaacagaaa gtgatcgatg
     8821 gatattatca aggaagaata gcctcataaa aagagttaat gaatcatttg aagattatga
     8881 accgacaaaa gccgttcggg caattcaaaa ttttgtcgtc aacgatctta gtaattggta
     8941 tgttcgctta aaccgaaaaa gattttggaa aggggagtac aatgaagaca agaaagctgc
     9001 ctaccagact ttgtatgaat gccttgtagt tgtggcaaaa cttgggagtc ccgtcgctcc
     9061 attttatatg gatcgcttgt acatggatct gaacaaggtt accggcaggg aatctgcgga
     9121 gtctgttcat ctgtctgatt tcccttcagt taatgatagc cttatggaaa tggagttgga
     9181 gcaaagaatg gagctggccc agggtataag ctcactggtt cacagtatta gaaagattag
     9241 taaaataaaa gtgcggcaac ctctatctaa aattttgatt cctatccagg gcgcgaaatt
     9301 taaaaatcaa attgaagcgg tacgggattt gattctaaac gaagttaacg tgaaggaaat
     9361 cgagtatatt gatgatgctt ctgatgttct ggtgaaaaaa gccaagccaa atttcagaaa
     9421 attgggaaaa gaatatgggc cgcagttgaa agaactatcg gaagccataa gtaattttac
     9481 cgcggaagaa ataaaaaaaa ttgaaatagg tgaaactgtc ggcgtaaata cgagcaaagg
     9541 aaaggtgaat ctcactcttg atgatattga agtattttct gaagatattc ccggatggtc
     9601 tgtagccagt gagggtaaaa ttacggttgc attggatgtt accgtctcag atgatcttaa
     9661 aatggaagga attgccagag aattggtaaa cagaattcaa aatatccgta aagataaggg
     9721 aatggaagtg caggataaga tttctattca aattgagaag gtttcagagc taatttccag
     9781 atcaatatta aaatttgaaa attatatttg taccgaaacc caggcgttat ccatgaaact
     9841 ggttgaagaa cttgctgatg gcgagatact ggaaatggat gaggcaaatg taaaagtgct
     9901 ggtttcaaaa gttacaacat gatcgaaaaa gacaatattt tcaaatactt cctgctcacc
     9961 ctggcgatca tctttttaga tcaggtggta aaagtgtctg tgcacaccta tatggatctg
    10021 ggtacaattg gccaaatcaa agtttttggt gaatggttta aactgcacta taccttgaat
    10081 cccggaatgg cctttgggat gcagcttggc tctccttatg gaaaattatt ccttaccatt
    10141 ttcagacttt ttgccatggt cggcattgct tactatttat attacctggc aaggaaaaag
    10201 gtccatccgg gattgttgtg gtgcattgcc ctaatccttg gtggggccgt tggaaacgtc
    10261 atagacagta cattttacgg tgtaattttt aacaacgctc cttatgatgc cagcactccc
    10321 tggtttcacg gacaagtggt cgacatgttt tacattgata tttgggaagg ccaggttgct
    10381 gattggattc ctttgtgggg aggtcaatac attgcactat ggcctatttt taatattgcc
    10441 gacgccagca ttttctgtgg tgtaaccctg atattaataa tgcaaaaaaa attctttgta
    10501 gagcatgaaa aagaaactaa agttgctgaa gctgtgaatg agatgggtga gtctaaggca
    10561 actcaactac cggatgaatc aaaatctatt gattaaagaa gaaccaaatc cgttatcacc
    10621 tttctgccaa attctttttc aaaaatctca agaatctttt ctttggaaga atttagttca
    10681 tgcttgagtg cagccgaatt aagctcagcg tataaaatgc gattttttat gaataatttt
    10741 tccgtcctct ttgcaattgt cttgcccaat agcctctccc aggagcttat caaggcggct
    10801 tcggaatact tgtccttaat ttgataggtt tcaagcaaat cctcgaaagc attcttaata
    10861 gtgttttcgc tataagccga tgacttcttt ctcacaaatc aaatatagaa acatctaaca
    10921 tgcttttata atgttattgt caatacatat gaatttcgcc ttgattttat tttttttgat
    10981 gatatcacgg ctcctctttt gccgggcatc agtcataaac acttgcccaa aatcaggact
    11041 tttcataacc ttcataaacc tggaaatccg tctgtcatca agtttatcaa agatatcatc
    11101 caataagagt aatgggagaa ttttcttttc ttctttcaag aattgatagt gagccagctt
    11161 cagagatatg ataaaggatt tctgctgacc ctgggaccca aactttttta gcccgtgaga
    11221 attaattttg aatataaagt catcccggtg gattccaagt tccgttctct gaagaattag
    11281 atctcgctca aaattattcc tgaaaacttt ttcaaaatcc ctggccttca cggaggattt
    11341 gtaactcagg ctaatttcct ctttgtcttc ggaaatgtcg ctgtagtatg aatttaagta
    11401 gggtttaaag ttctcaataa aattactccg gaaagtatgg ataacccggt tcaaatcaaa
    11461 taaactcttg ttataaactt caaacagagt tttgtccggt ttcttttttt cactcaacga
    11521 ttttagtgct gcgtttcgtt gtttcagaat attattatac ttcatcaagt ccgaaagata
    11581 atcctggcta aactgacaaa gtattccatc aatgaatttt ctccgcgctt ccgagtgttc
    11641 ccggaccaga tctgaatcat cgggagcaat cagcaccagg ggatatttac ccacatgttc
    11701 actcaatttg gggtaatcaa ctccgttaac tctaagccgc ttcttatttc cctttttcaa
    11761 cgaacattga attgaagcga agtcaccatt tgttcttaga tttccaagta ttgaaaaaaa
    11821 taactcacca tgattgatat tctgattatc ctggggattg aaagcgctct ttcctaatga
    11881 taagtaataa atggcatcca gtaaatttgt ttttcccgaa ccgttttctc caagaatgca
    11941 ggtaatccgg tcttcaaatt gtactgttat ttcacggaaa tttttgaagt tatgtagtga
    12001 cagagactct aaatgcaaat tccttcttct ttattgctcg aatttatgct attttcgcaa
    12061 tccggcataa gataccgatg ccttaaatcc aggttttatg ggaaaaacca aagaagcaaa
    12121 agaaagtaaa tcaaccagaa aacaaaaggc ttcctttgat gacaaaacct atatgttttg
    12181 gtatgagagc atgcttttaa tgaggaggtt cgaggagaag tgtggtcagt tgtatggaca
    12241 gcaaaaaata aaaggatttt gtcatttata tataggtcag gaagcaggag catcaggtgc
    12301 tatttccgca ttgaccaggg atgataaatg gattaccgcc taccgcgatc acgcacatcc
    12361 gctgggactg ggaacagacc caggcaaagt aatggctgag ctgtttgcca gggaaaccgg
    12421 agtctccaga gggaagggcg ggtcaatgca catgtttgat gtagaaagaa atttttatgg
    12481 aggacatggg atcgtggggg cacaaatacc cctgggtacc ggacttgcat ttgcagagaa
    12541 atataaagga aacaagaatt tgtgtatctg ttacatggga gatggagccg ttcgccaggg
    12601 tgcttttcat gaagccctta atatggcaat gcattataga cttccggtaa ttttcgtgat
    12661 tgaaaataat ggttatgcca tgggtacctc agtaaaaaga acttctaacg tgcatgattt
    12721 gtacacacta ggtgaagctt acgatatgcc ctccagatca gtagatggaa tgtcttgcga
    12781 agaagtgcat gaagcagtaa gtgatggcgc ggaaagagcc agaaacggga atggtccaac
    12841 tttgctcgaa ttcagaacct atagatacaa gggacattct atgtcagacc ctgccaagta
    12901 ccggaccaaa gaagagttgg aagaatacaa agccaaagat ccgattgaac gcgtaaaaaa
    12961 caccattctt aaaaataaaa tagcaacaga aaaggaattg gagaaaattg aagagaaaat
    13021 aaagcagcaa gttcaggatg ccgtaaattt tgctgaatca tctcccttcc ccccacctga
    13081 acaagctttc gaagacattt atatgcagaa ggattatcca tttataaagg attgatacat
    13141 tgattaatat cagcaatctt gctacttttg cgacctgtga ataattgaag aatggctaag
    13201 aagaataaga aaggaaataa gcaaggaggc gtcctggaaa gctctgaagt tcttcaggag
    13261 gagttcaccc ggtttgagca ttacctggaa acacataaat cactggtttt tgtaattgtc
    13321 ggcgtagtcc tggtggcaat tatttcgata ttgggatatc gctattacat ttctcagcaa
    13381 gatgaattgg ctcaaaagga aatgtttcag gcagtttatt attttgaatc ggacagcctg
    13441 gatttagcgc ttaatggtga tggaaaccac cttggacttc tggatataat tgatgaatac
    13501 ggaggtacag atgccgcgaa tctggcagaa tactatgcgg ggaccgctta cctgctgaag
    13561 ggtaattaca acactgctat tgaatacctg ggaagctttt cttctggcga catcttaatc
    13621 cagggaagag cttactcatt aaccggagat gcttacatgg aattgggaaa ttacggcaaa
    13681 gccgcagaat tttataacaa ggcagctaat tacaagccta atcagcattt cactccacaa
    13741 tatctgatga aagccgccct ggcttacgaa aaacttaatg attaccctac agcgaaggaa
    13801 atgtacgaca ggattgttga agagttcttt aacagttctg aatttcagga tgctaaaaag
    13861 caaagtgcaa gaatgcaggc gttaatttcc tcttagtggt aataaaaaaa ttattatggg
    13921 gtgccatgca tcccttattt gtttttgaat caataccagt acctttgttt tcatggcatc
    13981 tgctcaaaag aatctaagct cccattcccg gaggaatatc tttgatattt caaaaacaaa
    14041 atttggaatt gttatttctg aatggaatga tgatgttacc gactcgctct attcaggttg
    14101 catgcagacg ctgttggata agggtgccaa aagaggcaat attatcacac actacgttcc
    14161 cggtagtttt gaattaccac ttggagcaaa atggcttgca acggaaaaga aaattgatgc
    14221 ggtgatttgc ctgggtgctg ttattcaggg tgaaaccagg cacttcgatt tcatttgctc
    14281 agccgttgct cagggaatta tgaatgttgg tttggaattc agcaaaccgg ttgtctttgg
    14341 agtgctcaca actaatacaa aaaagcaagc gttggatcgc gcgggtggaa aacttgggaa
    14401 taaaggagat gaagctgcaa ttaccgctat caaaatgctt gggttttaag aagcaagcac
    14461 aaaaaataat atccaggtca ggaacagaac aggcagaaga attggaattg aaaagcgaat
    14521 aatatatcca aagaaagaag gcatcttaat tccaacttgt tctgctatag atttcaccat
    14581 gaagttaggg ccattgccaa tataagtcac cgcaccaaaa aaaaccgcgg caatggaaat
    14641 tgctttcagg tataaaatgg aatcataata aacaccaccc gctgcatacg cggtcacgtc
    14701 ggcatgattg ttaatacttg caccttcaga agccattgca gcagtgagga aattaatata
    14761 ggtaggtgca ttatcaagga atcctgacaa aattcctgta ccccaataca gggtatcgtg
    14821 gttgatcaac ttaaccccct ccggcgaggc tgcaaaatgt cccaccaatt ccaatgcagg
    14881 cagcattgtt ccgaaaattc caatgaaaat aaatgcaact tctttaatgg gctcaaaagt
    14941 aaattcattc cctttaagcg catttttatt agcaagtttg taactaagaa ttccaaccgt
    15001 gatcattatt atttcccgta caaatgaaag tgagttccct tgaatctcta tggatggaac
    15061 ccagtcaaaa acgttaggat caataaacac cgcgccaaca attaccatta gccagataaa
    15121 atttctactt cctttaattg ccaatttacc agtgtactta ggcggctcat catcaagatt
    15181 gtaataggag gaattcttct tatcaaagta atagaatata aggaggaata ttgtggtagc
    15241 catagcccag gctaaaacat tatgctcaag ggtccagaaa aacgggacac ctttcagata
    15301 tcccaggaaa atgggcggat ctcctatcgg agtaagtgcg ccaccaatat tgcttaccag
    15361 aaaaataaag aaaactatgt ggtaaggttt tatgcgatac ttgttcaatc ggataaatgg
    15421 cctgattaat agcatggatg ccccggtggt accaattaca tttgcaatta ccgctccaac
    15481 caggagtata aatacgttag ttgctggtct cgcctcctta tcaacttcga tcataattcc
    15541 accggaggca atatagagtg atcccaaaag ggctatgaat tgaatatatt cgaaaagatg
    15601 atggacgggg tcatgaatat tgtgaagaac aaagaggtaa taggcaacaa cgataacggc
    15661 caggattatg gatatagccg gataattttt atgccagaaa tgttcataga ataatgggcc
    15721 ggtagcaatg agaagaagca ggagaacgaa tggagtaacc agccacgctg gtgcagtggg
    15781 gtgatcttcc atcgaatcat ctgatgtttc tgatgatggg aaagaatttt cttcaacaga
    15841 tataccagcc gaagcatatg aatgggtcaa aagggatgtg tattctttgg atttacatcc
    15901 tccaagaata ataatccccc aaaataaggc agtaattact gatctcactt tataaaaata
    15961 aacagaaaca aattagggct accaattaat gaaattataa caactgttgc aaaagcaatc
    16021 tgagcaaaaa tctcctgaaa tgattactgt tggcaattca accgaacctc ctttctacag
    16081 cagtgggtat gcaaataagc atcttgcaag gttctgcaaa gcagaacggc agtttatacc
    16141 gaaaccaaac gccaacaacc aatgcaaatg gttttgtgcc gtcttttaga aaattgtatt
    16201 acgtagagga aagactttac aagagttgct aaaagatacg attgaaagtc attttggtta
    16261 ttagagaata tgggcaacct ggtgccaaaa atgcgttgaa gaactttcgc aaattctcca
    16321 cctttacgag aaatggaaat ccaaaggtgt agaagtagtc tctatatctt tggatacgga
    16381 caaagctttt tttaaaaatt tcacaagtat attcctttca taagtatgtg agattataga
    16441 aaatgggaca gtcaggcagt aaacgattat tatacatttg caacacctac tctatttttg
    16501 ttggacaaag atcagaagat tattttacga cctgattcgg ttaaacaggt agatgcctgg
    16561 gtggattttt atttaaaaga aaacaaagag tgaaaacaac caacgaaacg ccaacacatt
    16621 tataattaat tgctgtcaca gtgcggtttt cagcgtttca gcttccaacc ttatattgct
    16681 tttccaaaga acaacacggg tagagatgta atgattggca caccatcata tatccacaaa
    16741 agtcaattgg atgagcttgg aattacagtt ttgagcaaga aaaagtaaaa ataaagagta
    16801 tttgttacac ggttaccagt ttttgggaaa ttcttaccta tcattttaac tcatttccct
    16861 cacttttgac agggtttagt ttaaatctat acttacaaaa aagttagatt aagtgggtag
    16921 gcgttgaggg aagacgtccc ttcgtccatc cacccatttt tagcaaacct tttaacctgc
    16981 tatctgaatt atctccttag attatctcct actggagata attctttttt ataccaatct
    17041 aataattttc taagcctcaa attctccttt ttttgttaga tgttacataa ttcccaaaga
    17101 ttgggaaaat ccaacaattc aatttccctc tttttcttca actttaagat tcaacaaact
    17161 caggttatgg aaaatttgct gttgtttcat cttatgattg gtcctgttgt tttgggactt
    17221 tcatttttat ataaatacta cccgaatata caagcggttt tttggaagca aaaaattgac
    17281 aataaagaac agactatttg gagagaagcg gttagatata gtgcaaatgc catgatcatc
    17341 gggtctttct tcacaattat ttttcaaacc atttctgtgc taacaatgca gccttccagc
    17401 tcaatatggg ccagttcaat ttttcttgga tcgtgcttat tgggaatttt gccattaaca
    17461 gagattcatt tacggaaaaa atactggtat aaagactaat attcctgata agaaggccct
    17521 atttagtcca atttattctt ttatatttac gcctcagcta tcatttgtct gaatgaaagt
    17581 aattattgcc ggggcaggtg acgtcggatt ccacttggca aaacttctgg cctacgaaga
    17641 acacgatatt gttctgattg ataaggacag cgagaaattg aaagccgcat ccaactccct
    17701 tgatgtatat acaatcaaag gaaatgcaac ctattttgat gttctggctg aggcaaatgt
    17761 cgaagaagca gatctgttta tcgccgtaac ccaatctgaa gaaaccaacc tcactacttc
    17821 cataatcgcg aaaaaacttg gggcaaaaaa aactgttgcc cgaattcaga atacagaatt
    17881 cctattcaga aaagatgttt tggatttaag agatttgggt atagatgaaa ttatttctcc
    17941 ggaatcgctt gctgcccgcg aaataaacag attactaaag gaaacagcat taacagatac
    18001 ctttgttttt gagcagggtt tactttctct aattggggtt atagttgatg aaaacagtga
    18061 acttacaaaa aagtcgcttt ctgaaattag tgaaagccag gaccatgagt ttattaatgt
    18121 tgccatcctg agagataatg aaactaaaat cccgcgcggg atgtcaagac tcttgcccaa
    18181 tgatcatgct tattttgtta ctattccctc tggagttgac aaagtcgtaa gccttgcagg
    18241 aaagcaaaag atcaacataa agaacatcat gattcttgga ggcagcaggg taggatatca
    18301 tgcggcaaaa aaacttagca aaaagtttaa agtaaagctg attgaaaatg ataaggataa
    18361 agcgtttgaa ctggcagaca tgctccctga tgtgatggtc attaatggag acggacgaga
    18421 tgtggaactt ttactcgagg aaggaattga aaatatagat gcattcattg cggtaaccgg
    18481 taattcagaa actaacatta tctcctgtct ggttgccaag tctaacggag tcaagaaaac
    18541 aatttcattg gttgaaaaca tcgattatat tcacctttct caaaatattg gtgttgatac
    18601 catgattaat aagaaactaa tcgcggctaa ttttattttc aggcacataa gaaggggcga
    18661 tgtgatttca ttagcaagta tccatggagt agatgccgag atccttgaat ttatggtgaa
    18721 tgaagattca aagatagcca ataaggtaat taaggaaatc gattttcctg ccaatgccat
    18781 tattggcgga gtaataagga ataatgaggg tcatattacg atgggtgact ccgttctgct
    18841 tcccgatgac cgtgttgttg ttctatgtaa accggattgt atcaggaagg tcgaagaatt
    18901 ttttaaatga gaccacacct aaaatttata ataaattttc tgggattgct gctcgtgctg
    18961 aatggaattt tcatgttatc ggccgtcccc tttgcctggt attataatga accaattact
    19021 cctatcgtat attccgcact tatttccata attgtaggat tgcctgcctg gctggcaata
    19081 aaaattgaga aaggccggga tatccggaag cgggacggct atctgattgt aacactggga
    19141 tggttgatta tgtctttttc aggaacattg ccatttatcc taagcggcgc cattccaaat
    19201 ttcactgatg ctttttttga aacaatttcc gggtacacaa ccacgggaag cagcatcctg
    19261 aatgatatcg aatcactgcc gaaatctatt ttattatgga gaagtcttac ccagtggatt
    19321 ggtggcatgg gaattattgt tcttacgata gcgatactgc caattttggg aattggagga
    19381 atgcagttat ttatggcaga ggcacccgga attactgccg ataaattaaa gccccgaatc
    19441 caggataccg ctaccagatt atggctcatt tatgtgatgc ttaca
//
LOCUS       DASWHX010000148        13574 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_2736824, whole genome shotgun sequence.
ACCESSION   DASWHX010000148 DASWHX010000000
VERSION     DASWHX010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 13574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13574
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2736824"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..289
                     /locus_tag="VGA21_10025"
     CDS             <1..289
                     /locus_tag="VGA21_10025"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEX9650887.1"
                     /translation="DVLRTVGGIQVSGVGSDVTVSIRGQQTIMLNTQPLYVIDGLPIG
                     NDYSMANNAVRPSDIKSIQILRDKAQTLKYGEPGTNGVVVIKTKSGDTAKK"
     gene            complement(604..1065)
                     /locus_tag="VGA21_10030"
     CDS             complement(604..1065)
                     /locus_tag="VGA21_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015332540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX9650888.1"
                     /translation="MINEELIKTFYKAFQNKDFRTMQNCYADKAKFSDEVFVNLNANE
                     VRAMWEMLIKKGRDLKVEFSHIRVSENSGKAEWKATYTFSKTNRKVINRIKAEFIFED
                     GKIISHLDSFSFHSWARQALGVPGLLLGWSPWLKKKVRRNAMAGLKDYLRT"
     gene            complement(1117..1662)
                     /locus_tag="VGA21_10035"
     CDS             complement(1117..1662)
                     /locus_tag="VGA21_10035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650889.1"
                     /translation="MKFKSIWPRMSVATFFLFLVAANVTCAQSQEYLDKVQTLDSTIN
                     TLYSVISGEKGQERDWKLMRFLFHPEAKLIPAGKNNEGKYQARYLTTDDYISTSGNWL
                     VENGFIEKEIHRVTNTFGNITQVFSTYESFRSEADAKSFSRGINSIQLLNDGQRWWII
                     NIYWTSESAENPIPEQYLPRN"
     gene            complement(1701..5924)
                     /locus_tag="VGA21_10040"
     CDS             complement(1701..5924)
                     /locus_tag="VGA21_10040"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HEX9650890.1"
                     /translation="MKRVSLIISFLLVSGWAISQGNKKYDTQVIKAESAYDIGNYWTA
                     IKVNEKLGKKAIKKEGPESYYAGVYYILRGKYNLAVGMLNGFESSLEKGLEIALKNKG
                     SDPTNYVNQLLTVTELYIRFGNFLKADNLLESARKELTDADRMTENFKAGMNVFQAEI
                     QAGMGYYNKAMEFIVNSEDYFQGRAVDKETYVDPKSGKLRTERLSPNVVDERYSDYAS
                     ILTLKANVLRKQGSIIEADNAFGFAENWIQGKLGKGHIKYVQNQVLWTEMLEENGTQD
                     LPEANYRQALNLVKREHVESHYTAQALYEHLLRNYLMDGKEAKYRSLRTEYEHVIKKY
                     FDKGSMYYVNLNTLNFDVSVYRERTSGLEDQAAKILAGTAIPEFHPKRISLLEFLHSI
                     AVGNKKYKNGESYLNEILTIKKELYGANSPEYHLSRIDLANHYLDYTDNFKEAEEIYN
                     TSFIEVIEKEISSGHIKYVEILNHLASFYEANDRYDLASQTLDKALLATRAKYDNRDV
                     NFGVELEKIANLQISIGEYDKANENLTTALDILAKERKDEYDVVYYIKALETSARYKA
                     LMGMFDEAEADLIQSIKLKSRARATIDFDEVAANEGLASMYMDLGRYRSSEELLTSVI
                     ASKSALFGSSSRRLISPLTDRGRLFLINGDYTNADKTARRANSLGVQSYGENSTKIAN
                     SLLLISDVSYSIGDYEQAEANVLKAIEIQKKQFGEEHIDVAKSLSRLALIKFYKRDNP
                     KEVEKLFQSARTILENKLGNRNPQYAELLKNTAIMYIATKQFDNAFNSLGLAETIWLN
                     KAGRRNNINAASIYMLLGDIHYEQKNYKEANLQYEKAKKLYEKFFSRTHPEYVKVLSR
                     LSKVYYMEDDLSRAKQALDQTIKTYNDFIKTYFPALSEREKAKFWNTIKYDYEFYNTL
                     VLRMGERNPEMVRQMFNNALGTKALLLNTSLKIKERINTSTDSTLIRTYNIWSEKKDI
                     LTNTLSMSNEELLQNEIDAAKLSQEVEDLERQLSERSELFSSNLEEKQVTWEDVRKVL
                     GPNEVAVEMVRFRLFDHTFTDSVLYASLYLKNLETQPGPLMLLMKEGKEMETSYFKFY
                     RNSIRFRISDPYSYRVYWKPLEDRIGGNATVYLSADGVYNQINLEAIPTSDGKYVIDN
                     SNIILVSNTKDIYFNKIKYRTVQEENLASMFGNPKYYLASAGQQKFGQLPGTEKEIEE
                     LKSLLEKSGWNASEYLGSDATEPQVKQINNPKVFHIATHGFFTPSTEITENDQGLNQN
                     ESELFNNPLLRTGLMLRGAGDLLNKTAFNYNIEEGILTAYEAMNLNLEKTDLVVLSAC
                     ETGLGDLEFGEGVYGLQRAFIVAGARTLIMSMFKVNDEATQKLMVNFYNKWLQTGKKR
                     ESFVAAKKELRNEYQDPIFWGAFIMIGLD"
     gene            complement(5976..8099)
                     /gene="scpA"
                     /locus_tag="VGA21_10045"
     CDS             complement(5976..8099)
                     /gene="scpA"
                     /locus_tag="VGA21_10045"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009583656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HEX9650891.1"
                     /translation="MRPDFSKLKLEEKVAENPGPGAEGYQTSEEIPLRREYFNGDLKN
                     VGHIGFGAGIPPYLRGPYATMYIQRPWTIRQYAGFSTAEDSNAFYRRNLEGGQKGLSV
                     AFDLATHRGYDSDHPRVKGDVGKAGVAIDSVEDMKILFDQIPLDKMSVSMTMNGAVIP
                     IMAFYIVTAEEQGVPPTLLSGTIQNDILKEFMVRNTYIYPPVHSMRIVSDIFKYTSKK
                     IPRFNSISVSGYHMHEAGAPADLELGYTLADGLEYLRTGISSGLDIDDFAPRISFFWA
                     IGMNHFMEIAKLRAGRLLWAQIVKSFNPKNPKSMALRTHCQTSGWSLTEQDPYNNVIR
                     TCNEALSAVLGHTQSLHTNSLDEAIALPTDYSARIARNTQIFLQNSIGITNVVDPWGG
                     SYYLESLTHQLASKAWEHISEVEELGGMTKAIDSGLPKLKIEEAAAKKQARIDSAKDI
                     IVGVNRYQLSEVPDFELLEVDNTSVREQQIARINKLKSSRDNSKVLSSLDALTKCAET
                     GNGNILELAVDAARNRCTLGEISFALEKVFGRHRADTKAISGVYSAEIMGNKSFENAR
                     KAIEEFQKAEGRRPRILVAKMGQDGHDRGSKIIATSFADIGFDVDIGPLFQTPEEVAK
                     NAVENDVHLVGVSSLAASHKTLVPELILELEKAGRPDIKVVVGGVIPPNDHDFLYKSG
                     VIGIFGPGTVISAAAVNIIKRLMAN"
     gene            complement(8089..9225)
                     /locus_tag="VGA21_10050"
     CDS             complement(8089..9225)
                     /locus_tag="VGA21_10050"
                     /inference="COORDINATES: protein motif:HMM:NF013781.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEX9650892.1"
                     /translation="MPESKRKNLPQWNFRFTSKREWLDAARNELKEKKPGALRFNYGD
                     GLISEPYYDKNDLLYVSSNHYECFTPFDFQSEYQNFYCVPVKKGRGANDIIQSALSLG
                     MDSICLIQKGFNAHDLETALNEVVTDILNFEFQSESADEVAQWLFKEKIRINNGSIAK
                     LPSIEVLKELIEWSSNKEAFFPINLIAESPSDCANSNANLLSQLVDIIENLSNSGMPI
                     QTILAKLKFSFQFGLNFYQDASRIRAFKVVLEQVLKKYNSEVPSSIIIHGIMSNWKNE
                     KLGPHESMLKGTTNMISALVGGCNSFQVVPSDYDNEMQRRIAWHTFNVLSCEAHLDKV
                     KDPGRGSYFFENMTWQIAEKSLEKFREIESMGGYSRIQKQKNEA"
     gene            9497..10915
                     /gene="dnaA"
                     /locus_tag="VGA21_10055"
     CDS             9497..10915
                     /gene="dnaA"
                     /locus_tag="VGA21_10055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008631419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEX9650893.1"
                     /translation="MLKDCSAVWKDCLSLIRSQIPEQSFNTWFSPIIPVRLSEEILTI
                     QVPSQFFYEWLEEHYVNVLKKAITSVLGPAGKLEYSVIVDKGNAQSNPLTVKFPNGSG
                     NRRGFDSKASNHYKSPFHLKSVDLLHQDSQLSSRYTFDTFVEGDCNRLARSAGFAVAK
                     KPGITSFNPLMVYGGVGLGKTHLAHAIGNEIKRNIPEKFILYVSSEKFINQFIRAVQN
                     NEVQDFTNFYLQVDILIIDDVQFFAGKEKTQEIFFHIFNHLHQSGKQIIMTSDSPPRD
                     LKGLEERLLSRFKWGLTADLQQPDFETRIAIIRNKMEAEGLNIPEDVVEYLAYSVDTN
                     VRELEGVLISLIAHASLTRAEVDLELAKQTLKNIIHDIESEVGIDYIQKTVSEYYKID
                     LNDLKAKTRKKEIVIARQIAMYFSKEYTNHSLKSIGYHFGGRDHSTVIHAVQSVNDMI
                     DTNNNFKSSIEDLKKRLKMRSN"
     gene            complement(10916..12373)
                     /locus_tag="VGA21_10060"
     CDS             complement(10916..12373)
                     /locus_tag="VGA21_10060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020527152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX9650894.1"
                     /translation="MRKGIAIGMLSILSGFSGAFLYDYYKDERGQITALNINEPEPMS
                     EFTYPARVAPTELSEDFVKASAVSTASVVYLRNISEQAVSTSFFDLFFGEPGRVSETV
                     STGSGVIFSRDGYIVTNNHVVEHAKRIEIIHNKKTYDAILVGTDPSSDLAVLKIDASS
                     LPAIPMGNSRDLQVGEWVLAVGNPFNLTSTVTAGIVSAKGRNINIVRGKFPIESFIQT
                     DAAINPGNSGGALVDKNGDLVGINTAILSRTGSYAGYGFAVPIDIVRKVVRDLIEYGE
                     VQQSFIGAEITDIDERIANNLGIELDDSHYTGVAIVSLQQDGAASKGGLRKGDIIVEM
                     DGALIDGKGMYDEVLSTHSPGDRLVVAFERKGERKVANITLTNREGTTEIIKSEIYSS
                     RTLGADLESVSKVEKDRLEIEHGVRIKNVYRGSDRGLMSDLGIKENFIITHINSYPIK
                     SPDDLSNVLAKIKGRVRIEGVDENGVKGYYSFYFR"
     gene            complement(12475..13215)
                     /locus_tag="VGA21_10065"
     CDS             complement(12475..13215)
                     /locus_tag="VGA21_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008626652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEX9650895.1"
                     /translation="MELFMHGETWIALLTLTFLEVVLGIDNIIFISIVSNKLPEKMQG
                     KARVVGLSMAMVIRILLLLSITWIIGFTKPLFTLLSFEVTGRDIILLIGGLFLIAKST
                     GEIHQKLEGESRDTGSQSATSFKLVILQIIMIDIIFSFDSILTAIGLTRHVIIMIIAV
                     IISMIIMMTAAGKISAFINKHPTLEILALSFLILIGFMLAIEAFHYHVPKGYIYFAVA
                     FSLLVEFLNMKLRAKPEPVKLRKRIESE"
     gene            13369..>13574
                     /locus_tag="VGA21_10070"
     CDS             13369..>13574
                     /locus_tag="VGA21_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009582040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEX9650896.1"
                     /translation="MASLKEIQVPISNEMKEFEKKFRSSMKSNVVLLDRIMGYIVRRK
                     GKQMRPMFVFLTAGMCGGITESTYR"
BASE COUNT         3855 a         2871 c         2560 g         4288 t
ORIGIN      
        1 cgatgtacta agaacagttg ggggcattca ggtctcagga gtaggaagcg atgttactgt
       61 ttccatcagg ggtcaacaaa ccattatgct aaatacccag cccctttacg ttattgacgg
      121 gttgccaatt ggaaatgact attcaatggc taacaatgcg gttagaccgt cagacattaa
      181 atctattcag atattgcgtg acaaggccca gacactgaag tacggtgagc caggaacaaa
      241 cggtgtagtc gtaattaaaa caaagagcgg agacacagcc aagaagtagc tcgaaagaac
      301 taagaactta gatagtttag ttagtagccc ggataaacag aatccgggtt attttttgtt
      361 aatcagacat tggaggcgcc catagcggca gatgttattt agtatgtcat ctttgccgcc
      421 ccagtcagca catgcggtaa ttaccctggt ttccggtatt gctgtcttac agcaattaat
      481 aaacaggggc agaaaccgga atgacaattt ccagaaagaa aatgctcagc tgttatttag
      541 tatgtcaaaa aatagcaggc tatgtttttg ataactcata gcggcatgaa acgggaatga
      601 cattcaggtt cttagataat cttttaatcc tgccatcgca tttctacgaa cttttttctt
      661 tagccatgga gaccatccca gcaaaagtcc cggaactcct aatgcctggc gagcccaact
      721 gtgaaagcta aaagagtcaa ggtgactgat tatttttcca tcttcaaaaa tgaactcagc
      781 cttaatccgg ttaataacct ttcgatttgt ttttgaaaag gtgtacgtcg ctttccactc
      841 ggcctttcct gaattttcac tcacccgtat atgagaaaat tcaactttca aatctcttcc
      901 cttcttaata agcatttccc acatcgctct tacctcattt gcattcaggt ttacaaaaac
      961 ttcatcgctg aatttcgctt tgtccgcata gcaattttgc atagtgcgaa agtccttgtt
     1021 ctgaaaggcc ttgtagaatg ttttgattaa ttcttcgttg atcatttttg tgtggtaatc
     1081 atctctttga taaggcgaaa tttcaaagtc tggaaattaa ttgcggggaa ggtattgctc
     1141 cggaataggg ttttccgccg attccgaggt ccagtaaata ttaattatcc accacctttg
     1201 tccatcattt aatagctgga tgctgttgat ccctcgagaa aacgactttg cgtcggcttc
     1261 acttctgaaa gattcatagg tgctgaaaac ttgcgtgata tttccaaaag tattggttac
     1321 cctgtgaatt tctttttcaa taaaaccatt ttcaacaagc caattgcccg acgttgagat
     1381 ataatcatcg gtagtgagat atctggcctg atatttaccc tcattatttt taccggcagg
     1441 aatcaacttg gcttccggat gaaacagaaa cctcattagt ttccaatcgc gttcctgacc
     1501 tttttctccg gaaattaccg aataaagagt atttattgtg gagtcaagcg tttgaacctt
     1561 atcgaggtac tcctggctct gtgcacaggt aacgtttgct gcaaccagga aaagaaagaa
     1621 agttgccaca ctcatgcggg gccaaattga tttgaatttc atgcctttgg tttatattct
     1681 tttgaagtag atataaaata tcaatctagt ccaatcataa taaatgcacc ccagaaaata
     1741 ggatcctggt actcatttct aagttctttc ttggctgcta caaatgactc ccgttttttt
     1801 ccggtttgga gccacttgtt ataaaaattg accattaatt tttgggtagc ttcatcattt
     1861 accttgaaca tactcatgat cagggttcgt gctccagcta cgataaaagc gcgctgtaat
     1921 ccataaaccc cttcgccgaa ttccagatca cccaaaccgg tttcgcaggc tgacaacacg
     1981 acaagatccg ttttttcgag attcagattc atggcttcat aagccgtaag aataccttct
     2041 tcaatattat aattaaaggc ggttttgttt aacagatcgc ctgcacccct aagcataagt
     2101 ccggttcgca ggagtggatt attaaataat tcggattcat tttgattcag accttggtca
     2161 ttctcagtta tttctgttga aggagtaaaa aaaccatgag tggctatgtg aaatactttc
     2221 gggttattaa tttgtttgac ctgaggttca gttgcatcag aacccaggta ttcacttgca
     2281 ttccagccgg acttttctaa gagagatttt aattcttcaa tttccttttc tgttccaggt
     2341 aattgtccaa atttttgttg acctgcagag gcaaggtaat attttggatt tccaaacatg
     2401 ctggcaagat tttcttcctg gacagtacgg tattttattt tattgaagta aatatccttg
     2461 gtgttgctaa ccagaataat attagaatta tcaattacgt attttccatc gctcgtggga
     2521 attgcttcca ggttaatctg attgtaaacg ccgtcagcag aaaggtaaac tgttgcattg
     2581 ccgccgatcc tatcttccag gggtttccaa tatacccgat aggagtaggg atcagatatt
     2641 ctgaacctga ttgaattcct gtaaaactta aaataactgg tttccatctc tttgccttct
     2701 ttcatcagaa gcatcagggg acccggctga gtctccaaat ttttgagata cagggatgca
     2761 tataaaacgg agtccgtaaa cgtatgatcg aataacctga atcgaaccat ttctacggcg
     2821 acctcattcg ggccgagtac ttttcttaca tcttcccagg ttacttgctt ttcttcaagg
     2881 ttgctgctga ataattcaga cctttcactc aattgtcttt ccaggtcttc aacctcctgc
     2941 gatagctttg cagcatcaat ctcgttttga agcaattctt cattgctcat cgacagcgta
     3001 ttcgtgagaa tatccttttt ttccgaccaa atattgtagg ttcgaataag ggtggagtct
     3061 gtactggtat taattctctc ttttattttc aaggaggtgt tgagaagaag agctttcgtt
     3121 ccaagagcat tgttaaacat ttgccttacc atttcaggat tccgctcacc cattctcaga
     3181 accagtgtgt tgtaaaactc ataatcatac ttaatggtat tccagaattt ggccttctcc
     3241 ctttcactca gagcagggaa gtatgttttg atgaaatcat tataggtttt aattgtttga
     3301 tccagtgctt gcttggctct gctcaaatca tcttccatgt aatacacttt gctcaggcgg
     3361 gatagaactt tgacatattc tggatgtgtt ctggaaaaaa acttctcata cagtttttta
     3421 gctttttcat attgaagatt cgcttcttta taattctttt gctcgtagtg aatatcccct
     3481 aaaagcatat agatggatgc tgcattaatg ttatttcttc tgcctgcttt attcaaccag
     3541 atggtttcgg ccaatccaag agaattaaaa gcgttatcaa attgctttgt tgcaatgtac
     3601 atgattgccg tgttcttcaa caattcggca tactgtggat ttctattgcc caatttattt
     3661 tctaaaatgg ttcttgctga ttgaaagagt ttctcaactt cctttgggtt atctctctta
     3721 taaaatttta ttaaggcgag tcgggacaac gatttggcaa catcaatatg ttcctctccg
     3781 aattgtttct tttgaatttc aatcgctttt aaaacattcg cctctgcctg ttcataatct
     3841 cctattgaat aactcacgtc agaaatcaaa agcaaactgt tggcaatttt ggtagaattt
     3901 tctccgtagc tctgaacgcc aagcgaatta gcgcgtctcg cggttttatc agcattcgta
     3961 tagtcaccat tgatcaaaaa caaccggcct ctgtctgtga ggggtgaaat taatcttctg
     4021 ctggagctgc cgaaaagcgc gctcttactt gcaataacgg aggtcaataa ttcttccgag
     4081 ctgcgatacc tccctaagtc catgtacatg gaagccagtc cttcattggc cgcgacttca
     4141 tcaaaatcaa ttgttgcacg ggcccggctc tttagtttga tagattgaat caggtctgct
     4201 tccgcttcat cgaacatgcc catcagggct ttatagcggg cactggtttc gagtgcctta
     4261 atatagtaaa ctacatcata ctcgtcttta cgttcctttg ccaggatatc caacgcggta
     4321 gttaaattct cattggcttt atcatactcc ccgatgctta tttggagatt agcaatcttt
     4381 tccagttcaa ctccaaaatt tacatcccgg ttatcatact tggcacgggt agccaataag
     4441 gctttgtcaa gggtttggga agcgagatcg tatcggtcat tcgcttcgta aaaacttgcc
     4501 aggtgattta atatttcaac atatttaata tgacccgaag aaatttcttt ttcaataact
     4561 tcaatgaaac ttgtattata gatttcttcc gcctctttaa aattgtcagt ataatctaag
     4621 tagtgatttg ctaaatctat tcttgaaagg tggtattccg gtgaatttgc tccataaagt
     4681 tcttttttga tggtaaggat ttcgttaagg taggactcgc catttttata ctttttatta
     4741 ccaactgcaa tggaatgcaa aaattctaac aatgaaattc ttttcggatg aaactctggt
     4801 attgcagtac ctgccaatat tttcgcggct tgatcttcca gaccggaagt tcgttcacga
     4861 tatacagata catcaaaatt taatgtgttg aggtttacat aatacatgct tcctttatca
     4921 aaatattttt tgattacatg ctcatattca gtcctcaaag atctgtactt tgcttccttt
     4981 ccatccatca gataattcct cagcagatgc tcataaagcg cctgcgctgt ataatgagat
     5041 tcaacgtgct ctctcttaac aagatttaaa gcttgcctgt agttagcttc cggaagatct
     5101 tgtgttccat tctcttccag catctcagtc cagagaacct ggttttgcac atacttgata
     5161 tgacctttgc caagctttcc ctgaatccag ttttcggcaa aaccaaacgc attatctgct
     5221 tctataatgg aaccttgctt tctgagtacg ttggctttaa gtgttagaat tgatgcataa
     5281 tcagaatatc tttcatccac tacatttgga gaaaggcgtt cggttctaag ttttccgctt
     5341 ttggggtcaa cgtatgtttc tttatctaca gctcttccct ggaagtaatc ttctgaattg
     5401 acaataaact ccattgcttt gttgtagtag cccatcccgg cttgaatttc tgcctgaaag
     5461 acattcatcc ctgccttaaa attctctgtc atccggtcag cgtctgttaa ttctttgcgt
     5521 gcgctttcca gaagattatc ggcttttagg aaatttccga aacgaatata aagttccgta
     5581 acagttaata actgattaac atagttagtt ggatcgctcc ctttattttt aagcgctatt
     5641 tctaatccct tttccaggct gctttcaaag ccattcaaca ttccaacagc aagattatat
     5701 ttacccctaa ggatataata aacccctgca taatagcttt cggggccctc tttcttgatg
     5761 gctttcttcc ccaatttctc atttactttt attgcggtcc aataattccc gatgtcgtaa
     5821 gcagactcag ctttaatcac ctgagtgtca tatttcttgt ttccctggga aattgcccat
     5881 ccggaaacta atagaaagct aatgattaat gatactcttt tcatctcact cacaattttg
     5941 atgatacagg atagctaagt tagttaaaaa gcggcttagt ttgccattaa ccttttaatg
     6001 atgttcaccg cggcggcaga gattacagta cccggaccaa atattcctat caccccggat
     6061 ttgtatagaa aatcatggtc gttgggagga ataacaccgc caaccacgac tttgatgtcc
     6121 ggtctgccgg ccttttccaa ttccaggatt aattccggca ccaaagtttt gtgactggca
     6181 gccagcgatg aaactccaac cagatgcacg tcattctcaa ccgcgttctt tgcaacttct
     6241 tccggcgttt gaaataatgg accgatatcc acgtcgaaac caatatcggc aaaactggta
     6301 gcgatgattt tggaacctct gtcatggcca tcctgtccca ttttggctac cagaatacga
     6361 ggccttcttc cttctgcctt ttgaaattct tcaatggcct tcctggcatt ttcaaaagat
     6421 ttatttccca ttatttctgc tgaatagact cctgagattg ctttggtatc agctcggtgc
     6481 cggccgaata ctttctctaa agcaaaagaa atttctccta atgtgcaacg gttacgggct
     6541 gcatccacag ccaattcaag tatattccca tttccggttt cagcgcattt tgttaatgca
     6601 tcgagagagg ataatacttt tgaattatcc cttgaagatt ttagtttgtt tattctggca
     6661 atttgttgct cccttaccga agtgttgtca acttctaata attcgaaatc aggtacctct
     6721 gacaactgat accggttaac tcctacaatg atatctttag cactatctat cctggcttgt
     6781 ttttttgcag cggcctcctc aatcttcaac tttggcaagc ctgaatctat tgctttcgtc
     6841 attccaccca attcttccac ttcagaaata tgttcccagg cttttgaggc aagctgatgg
     6901 gttaaagact caaggtaata ggaaccaccc cagggatcca ccacgttagt gatgccaata
     6961 gaattttgca aaaatatctg cgtgttcctg gcaatcctgg ctgagtaatc agtaggaaga
     7021 gcaatggctt catcaaggga atttgtatga agagattggg tatgccccag aacagcggac
     7081 agagcttcat tacaagtacg aattacattg ttatacggat cttgttcggt caaactccag
     7141 ccggaggttt gacaatgagt tctaagagcc attgactttg ggttcttagg attgaaggac
     7201 ttcactatct gagcccataa taatctgccg gcgcgaagtt ttgctatttc cataaaatgg
     7261 ttcattccaa tagcccagaa aaatgaaatc cggggagcaa agtcatctat atccaatcct
     7321 gaagaaatac cggttcttaa atactcaaga ccatctgcca atgtatatcc aagttcaaga
     7381 tctgccggcg ctccggcttc atgcatatga tatccgctga cacttatgga attgaagcgc
     7441 ggtatcttct ttgaagtata tttaaaaatg tcggatacaa tccgcattga atgcacagga
     7501 gggtagatgt aagtgtttct caccataaat tccttcagaa tatcattttg gatggtaccg
     7561 gataaaagag taggcgggac accctgttct tccgccgtta cgatatagaa agccattatg
     7621 ggtatcaccg ctccattcat ggtcatactt acagacattt tatccagcgg aatctggtca
     7681 aaaaggattt tcatgtcttc gacactatca atagcaacac cggctttgcc cacatcgcct
     7741 tttacccttg ggtggtcgct atcatatccg cggtgagtgg ccaaatcaaa tgccaccgag
     7801 agtcccttct gcccaccttc gagatttctc cggtaaaagg catttgaatc ttctgcagtt
     7861 gaaaatccag cgtattgacg aattgtccag ggtctttgga tatacattgt tgcatacggc
     7921 ccacgcaagt atggcggaat cccggcacca aatccgatat ggcccacatt tttcaaatca
     7981 ccgttaaagt actccctccg caacggaatt tcttcggagg tctgatagcc ttcggcgcca
     8041 ggcccgggat tttcagccac cttctcctct aattttagct ttgagaaatc aggcctcatt
     8101 tttttgtttt tgaattcttg aatatccgcc cattgattca atttctctga atttctctaa
     8161 tgatttttca gcaatttgcc aggtcatatt ttcaaaaaaa tatgagcccc tacccggatc
     8221 tttcactttg tccaggtgag cttcacagga aagcacatta aaggtatgcc atgcaatgcg
     8281 acgttgcatt tcgttgtcat aatctgaagg cacaacctga aacgaattgc atcctcctac
     8341 taatgctgaa atcatattag tagttccttt taacatgctt tcgtggggcc caagtttttc
     8401 gtttttccaa ttagacataa taccatgaat aattattgaa gagggaactt cagagttgta
     8461 ttttttcaag acctgttcaa gaacgacttt aaaagcccgg attcgtgaag catcctgata
     8521 aaaatttaag ccgaattgaa aactgaattt taattttgcc agaattgtct gaataggcat
     8581 gccagaatta ctcagatttt caattatatc aaccaattga cttaacaagt ttgcattgct
     8641 attggcgcaa tccgatgggg attcagcaat aaggttaatt ggaaagaagg cttccttgtt
     8701 tgatgaccat tcaattaatt cttttaatac ttcaatagaa ggtaattttg ctattgagcc
     8761 attattgatt ctaatttttt ccttgaaaag ccattgggcg acttcatctg cactttcgga
     8821 ttgaaattca aaattcaaaa tatctgtaac cacttcattc agagcagttt ctaaatcatg
     8881 cgcattgaaa cccttttgga taagacagat tgagtccatg cccagactaa gcgcagactg
     8941 gataatatca tttgctcccc tgcctttctt aaccggaacg caataaaaat tttggtactc
     9001 cgactgaaaa tcgaaagggg taaaacattc ataatggttg gatgaaacat agaggagatc
     9061 atttttatca taatatggct ctgaaatcaa tccatcgccg taattaaatc tcaaagctcc
     9121 gggcttcttc tctttcaatt cgttcctggc ggcatccagc cattcacgtt tcgaggtaaa
     9181 tctgaaattc cattggggta aattttttct tttactttcc ggcattatct tatggtaatg
     9241 gtaaaattat aaaaaatgtg caacccggca tgaacaattt taatgacgga aagaatgatg
     9301 attactttag gaatgaagtt agatactatt ttgtttttta aagaattttt tcgaaacata
     9361 tggtgtcact taattttttg gaacaaatta tcctgcgatt aaaattatta cttttttgtt
     9421 aagaaatttt tcgtcaactt tgaaaccctc ttaattcagc caggggaaaa tttatcaact
     9481 aagttttaaa gttctaatgc taaaagattg ttctgcagtt tggaaggatt gtctttctct
     9541 gataagaagt caaataccgg aacaaagctt caatacctgg ttttcaccta taatacctgt
     9601 tcgcctaagt gaagaaattt tgactattca ggtcccaagc caattttttt atgagtggct
     9661 ggaagaacat tacgttaatg tattgaaaaa agccatcaca tctgttcttg gaccggctgg
     9721 aaaattggaa tattctgtta ttgttgataa gggaaatgca caatcaaatc cacttacagt
     9781 gaaatttcca aatggcagcg gaaacaggcg tggctttgat tcgaaagcat ccaatcatta
     9841 caagagtcca tttcatttga agtccgtaga tttgctgcac caggattcgc agctaagctc
     9901 gcgatatact tttgatacat ttgtggaggg tgactgtaat cgcctggccc gttctgccgg
     9961 atttgcggtg gcaaagaagc ccggaatcac ttctttcaat ccattaatgg tatacggggg
    10021 agtcgggttg gggaagacgc accttgctca cgccattgga aatgaaataa aaagaaatat
    10081 cccggaaaaa tttattcttt acgtttcctc tgagaaattt atcaatcaat tcattagagc
    10141 tgtccaaaat aatgaagtcc aggactttac taatttctac cttcaagttg atattttgat
    10201 aattgatgat gttcagttct ttgccggaaa ggagaaaact caggaaatat tcttccacat
    10261 tttcaatcac ttgcatcaat ctggaaaaca aataataatg accagtgatt cgcctcccag
    10321 ggatctgaaa ggactggagg aaagattatt atcaaggttc aagtggggcc tgaccgcgga
    10381 tttgcaacag ccggacttcg aaaccaggat tgcaattata cgaaacaaaa tggaagctga
    10441 agggttgaat attccggaag atgttgttga atatttagcg tattccgtag ataccaatgt
    10501 ccgggaatta gaaggagttt taatttcttt aattgctcat gcatccctca ccagagcaga
    10561 agttgacctg gaattggcaa aacaaacctt gaaaaatatt attcacgata ttgaatctga
    10621 ggtgggaatc gattatatcc agaaaactgt ttcagaatat tataagattg accttaacga
    10681 tctgaaagcc aaaacaagaa aaaaagaaat tgtgattgcc cggcaaatag ccatgtactt
    10741 ctctaaagaa tacaccaacc attcacttaa atcaattgga tatcattttg gaggtcgtga
    10801 tcacagcacg gtaattcacg cggtgcaatc agttaatgat atgattgaca ccaataataa
    10861 cttcaagagt tccatcgaag atctgaagaa aagactcaaa atgagatcta attaattacc
    10921 tgaagtaaaa gctgtaatag cctttcactc cgttttcatc aacaccctct atccgcactc
    10981 gtcccttgat tttggccagg acattggaca ggtcgtcagg agatttaatg ggatagctgt
    11041 ttatgtgagt tataatgaaa ttctccttaa tgccaaggtc agacattaat ccccgatcac
    11101 tgccacgata gacattctta atgcggactc catgctcaat ttccaggcgg tctttttcta
    11161 ctttgctcac cgactcaaga tctgctccca aagtcctgga ggaataaatc tctgacttaa
    11221 taatttctgt ggtgccttca cggttcgtca aagttatatt ggctactttc cgttcacctt
    11281 ttctttcaaa agcaactacc aaccggtctc cgggagaatg cgtgctgagt acttcatcgt
    11341 acattccttt gccgtcgatc aaagcgccat ccatctccac aataatatct cctttccgaa
    11401 ggccaccttt tgaagctgct ccatcttgct gcaggctgac aattgcaacg ccggtgtagt
    11461 gactatcatc aagttcaatt cccaggttgt tggcaatcct ttcatcgatg tctgttattt
    11521 ccgctccaat aaacgattgc tgtacttctc cgtattcaat taaatcacgc acaacttttc
    11581 gcacaatatc tatgggtacc gcgaatccat atcccgcgta tgaaccggtt cttgaaagta
    11641 tggcagtatt tattccaact aaatcgccat ttttatctac caaagctcca ccgctgttcc
    11701 ctggatttat ggctgcatca gtttgtataa aagattcaat gggaaattta ccacggacaa
    11761 tattaatatt gcgacccttt gcgcttacta ttccggcagt cactgttgag gtaagattaa
    11821 atggattgcc aaccgcaaga acccattcgc ctacttgcaa gtccctggaa ttacccatgg
    11881 gtatcgcagg aagactgctg gcatcaattt ttaaaacggc caggtcagag ctgggatccg
    11941 tcccaacgag aattgcatca taagtttttt tattgtgaat gatttcaatt cttttggcat
    12001 gttcaaccac gtgattattt gtaacgatat aaccatctct ggagaaaata acccccgaac
    12061 ccgtgcttac cgtttcactt accctcccgg gttctccaaa aaacagatcg aaaaaacttg
    12121 tgctaacagc ttgttctgaa atgtttcgca aataaacaac gctggctgta cttacggcgc
    12181 tcgctttaac aaaatcttct gataattctg taggagcgac ccttgccgga tatgtgaact
    12241 cactcatggg ttcaggttcg tttatattca atgcggtaat ttggcctcgc tcatctttat
    12301 aataatcata caaaaatgcg ccactaaaac cactcagaat actgagcatc cctattgcta
    12361 ttcctttcct cattgcggta aatttatctc aatgcaaata ttgtgcatta ttaattacga
    12421 tacaaagccg ggattgttgg ttaaaaatta ctaaaacttc attcaataag aatctcattc
    12481 cgattcaatt cgctttctaa gttttactgg ctcgggctta gctcttaatt tcatgttcaa
    12541 aaattcaacc aataaagaaa atgccaccgc aaaataaata taaccctttg gtacatggta
    12601 atggaatgct tcaatcgcca gcataaatcc aatcaggatc agaaacgata atgcgagaat
    12661 ttccagggtc gggtgtttat tgataaaagc gctgattttg ccggccgcgg tcatcataat
    12721 aatcatggaa ataataaccg cgataatcat gataattaca tgtcttgtta aaccaatagc
    12781 agtaaggatt gaatcaaagg aaaaaattat atcaatcata ataatttgca ggataactaa
    12841 tttaaaactt gtggcactct gactccccgt gtctctgctt tccccttcaa gtttctggtg
    12901 gatctcaccg gtactttttg ctattagaaa taacccgcca attaataata tgatatcacg
    12961 gccggtaact tcgaaggata acagggtaaa taatggtttg gtgaacccga tgatccaggt
    13021 aatactgagc aacaggagaa tcctaatcac catcgccata ctcaatccga caactctggc
    13081 ttttccctgc atcttctcag gaagtttgtt agaaacaatt gaaatgaaga tgatgttgtc
    13141 tatacccaaa accacttcaa gaaaagtaag agtaagcagc gcgatccagg tttcaccatg
    13201 catgaaaagt tccataatat tttgttttta gaaatttgat gaaaattaaa actaaatcga
    13261 cgcaggaaca attgaaggag ttatatatta ttattttaga ctaatgttta agcgaacctt
    13321 tttctgcttt tacccagata gcattattat tgcaattaca aagacagaat ggccagtcta
    13381 aaagaaattc aggtcccgat ttccaatgaa atgaaggagt tcgaaaagaa gttcaggagc
    13441 tccatgaaaa gcaacgtggt tttgctggac aggattatgg gatacattgt tagaaggaag
    13501 gggaagcaga tgagacccat gtttgttttt ttaactgccg gaatgtgcgg gggcattacg
    13561 gagtctacct accg
//
LOCUS       DASWHX010000149        21951 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_2011646, whole genome shotgun sequence.
ACCESSION   DASWHX010000149 DASWHX010000000
VERSION     DASWHX010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 21951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21951
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2011646"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..245)
                     /locus_tag="VGA21_10075"
     CDS             complement(<1..245)
                     /locus_tag="VGA21_10075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650897.1"
                     /translation="MKRIPAFLILIIACSPIPALDNFNMEKWKNDEQGCKSLRKVEIS
                     SLMEQRTKLMGLSEEEIIKLLGNPDETNLYQRTQKFLV"
     gene            595..768
                     /locus_tag="VGA21_10080"
     CDS             595..768
                     /locus_tag="VGA21_10080"
                     /inference="COORDINATES: protein motif:HMM:NF037100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEX9650898.1"
                     /translation="MINEEQKNIIIEVLQPYYPKMIGVFGSFARNRNNHDSDLDILVE
                     FQDTVNLLDNHRS"
     gene            1108..1554
                     /locus_tag="VGA21_10085"
     CDS             1108..1554
                     /locus_tag="VGA21_10085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650899.1"
                     /translation="MNKFSKPFWLLSFFLTSVIFIYTYANMDDMVSYYQNPYTLSKLY
                     ISRELFFFIGLATIIVTNLALYVFSKLIRDSHVAKEYFKELGTWFITMAGISNIFLFV
                     SLVFILLFNRLDDFDPRVLGFFLYFIGFIAFAWLVRLIILLLKRQQ"
     gene            1856..3712
                     /locus_tag="VGA21_10090"
     CDS             1856..3712
                     /locus_tag="VGA21_10090"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008200547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEX9650900.1"
                     /translation="MAPGNYTEASIKSLDWKEHIRLRPGMYIGKLGDGSSPDDGIYVL
                     VKEIVDNAIDEYVMGFGRTIDIKISDQEVEVRDYGRGIPLGKVIDCVSKINTGGKYDS
                     EAFQKSVGLNGVGTKAVNALCKNFLVQSFREGKTKVAEFVRGEITKNPKIAANKSKSG
                     TLVVFKPDGNIFKNFHYIPEYLEEQIWNYVFLNSGLTINLNGKKFHSQNGLLDLLKRK
                     TDEDSLRYPIIHRKGKDIEFAISHSNQYGEDYYSFVNGQFTTQGGTHLAAFREAIVKT
                     IREFYKKDYDSTDIRASIIGAIAIRVQEPVFESQTKTKLGSQNIAPEGPTVRTFINDF
                     VGKELDNFLHQSPKVADALLKRIQQSERERKEIAGIKKLANERAKKANLHNKKLRDCR
                     LHYDEDKSGAETMIFITEGDSASGSITKSRDVQTQAVFSLRGKPLNSYGLTKKVVYEN
                     EEFNLLQHALNIEDGLDGLRYKKIIIATDADVDGMHIRLLLLTFFLQFFPDLVKHGHV
                     FILETPLFRVRNKKETIYCYSEGDKETAVNKLGGKPEITRFKGLGEISPDEFKNFIGE
                     KIRLEPVKVGRDSTVKQILEFYMGKNTPDRQEFIIKNLKVEKDIVKEEVVVA"
     gene            3714..6497
                     /locus_tag="VGA21_10095"
     CDS             3714..6497
                     /locus_tag="VGA21_10095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007656406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase/topoisomerase IV subunit A"
                     /protein_id="HEX9650901.1"
                     /translation="MAKRSRKKKSPDREEDLVHDLRPVSGLYKNWFLDYASYVILERA
                     VPAIEDGFKPVQRRIMHALKEMDDGRFNKVANVIGSTMQYHPHGDASIGDAIVNIGQK
                     ELLFDIQGNWGDVRTGDSAAAARYIEVRLSEFAKEVVYNNQTTQWQLSYDGRKREPVT
                     LPVKFPLLLAAGVEGIAVGLSTKILPHNFRELIKASIEVLKGKSPKIFPDFPTGGTAD
                     FSEYNQGYKGSRVRIRAKIEAIDKKTLIIKDIPFGTTTTGLIDSIIKANDKGKIKVKQ
                     VIDNTAEHVEILVHLAAGQSPDVAIDALYAFTDCEYSVSPNACVIIDEKPRFLHVNEI
                     LKINTEKTVALLKQELQIRKEELQERILFSSLEKIFIEKRIYRNIEECETWDAVIKTI
                     DKGLKPYKKQFYREITGEDIVKLTEIKIKRISRFDSFKADELMKKLEEELKETLYNLK
                     NLTNFSIRYYENLLEKFGKGRERKTESKSFDVITATVVAATNQKLYVNRKDGFAGYGL
                     RKDEFVSECSDLDDIIAMRSDAKFMVSRISEKTYMGKEIMQVAVFKKNDERMVYNAVY
                     VDGKTGKSMVKRFQVIGVTRDKEYDISKGAPGSKLLYISANPNGEAEVITVKLTQGSK
                     ARKKVFDFNFSQLDIKGRGAGGNIITRYPVRKIERKTTGVSTLKGLDIWYDESVGRLN
                     REGRGKLVGNFDGDDRIIVFYKDGHYEITSYELSNRYEYDKVVEVAKLRPDVIITAVH
                     YDGGSKNYYVKRFTVETSTLDKMFRFISEEPGSKLVLATTNPNPVAILEIEKGKGKTR
                     EAIQIDLTEVVDIKGWKAFGNRLSNYKIKNVKLAETVKSGAEFMEKPEEKPKLKKQKL
                     QKKVKTKSVKSKTFSKKAKTNASKEKSIPGKPVIRKSKSSKKKGKINNQQDLFASQNS
                     ESFEVGSTIQLDL"
     gene            6497..7183
                     /locus_tag="VGA21_10100"
     CDS             6497..7183
                     /locus_tag="VGA21_10100"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEX9650902.1"
                     /translation="MDQENNKRLLDFISPFVTIHKRERIEEVLTQRTRHLTVVLEDIY
                     QSQNASAVIRSCECIGIQDVHVIEQKNFYKVNRDVLRGAGKWISLHRYKTAGSAQDCF
                     STLREMGYKIIGAAPETIYSTIQEIDINHKLAIVFGTELEGLSDYALKNVDGMVRIPM
                     SGFTESFNLSVSAALVTFNLLTKVKTELQNWELTEREKLELRLEWYKKSIKNAVKLET
                     EFFRLYPGIS"
     gene            7180..7833
                     /locus_tag="VGA21_10105"
     CDS             7180..7833
                     /locus_tag="VGA21_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009196065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ElyC/SanA/YdcF family protein"
                     /protein_id="HEX9650903.1"
                     /translation="MKVVKKILKFLLVIFAVIFVFILASNFWIWNVTHHRVFESIDDL
                     PENEVALVLGTSRFTVGGKVNPFFKYRIQLAAELYKSGKVKYIIVSGDNNTKYYNEPK
                     EMRDALLLLGIPYRKIILDYAGFRTLDSMVRCKEIFGQKKITIITQKFHSFRAIFIGD
                     YYGMETTAIAARNLPFRQSFKVELRETLARSKAVIDLYILRESPKFLGEKEIIGQVR"
     gene            7909..10461
                     /locus_tag="VGA21_10110"
     CDS             7909..10461
                     /locus_tag="VGA21_10110"
                     /inference="COORDINATES: protein motif:HMM:NF039501.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5686 family protein"
                     /protein_id="HEX9650904.1"
                     /translation="MAKRTFVKVFFTLLFIIAFTRIFGQETIIKGRITDAKSGDPIPF
                     CNIFFQGTNIGTTSDFVGFYTIRTSTPKDSLVASYIGYKQRLKPVKFAVVQEINFQLA
                     EDIQQLKELVFVAEENPAFAILRNVVRNKNLNDKRSLDSYEYESYNKIELDLDNLTDK
                     FRERNIVKKVKKVIDSVDQIAGDDGRPILPFFISESISKAYYRKTPEQKKEHIIKTKI
                     SGVGVEDGTLISQVIGSTFQEYNFYQNWLNIWDKEFVSPIADGWRAYYDVYLMDSVMI
                     GNFFCYRLEVFPKRAGDLAFTGSIWIDKNTWALKQADLRIDKIANLNYIEKLKIQQEL
                     VPTSSGAWIANKSRVSTDFQFLGEFGISNQAAGILAKFYTSNKDIVVNQEYKRNFFDQ
                     AITMEEDVAEFEDNYWPENRHDPLTVAETNVIQMIDTLAEIPTIRRLSNTITYLSTGY
                     IDKLWIFELGPFPLFFASNNIEKFRFRLGGITSIRFSRKFVFSGYGAYGIRDDKWKYG
                     VGLDFIIDRKPWTKIGISRTYDIQQVGLTFDDVFSNIGLITFESYFRNIGHEMPFFLT
                     ENKINFQTDLKKGITQKLEVRQRDYNPINLDTTWNYSYRINPVEGGASPLRSNFSTSE
                     ISFESRWGKDEIWLIDDNKRFSLGPVKFPVITLRYTLGIKDFMGGDFNYQKVAVNIYK
                     PVKMGILGISNFNITGSKIFGAVPLPLLYGHLGNESPFYVSTAFSTMGFSEFFSDQFI
                     SLGYRHHFEGFVLNTIPLFKRLKWRLVANANILQGNVSAKNEDIHSDVGGNQLLPTQP
                     LRSLSSKPYVELGYGIENIFRVFRIDAFHRLTYLENRTGSAFALKFSLQFVF"
     gene            10501..11481
                     /locus_tag="VGA21_10115"
     CDS             10501..11481
                     /locus_tag="VGA21_10115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650905.1"
                     /translation="MVWNSTLLSTLLIGFFLLSCHSPRNEKVPDSKPLSSEVYKKQLS
                     ELEEPEGMLEKEKFFAKKAFLEEQSGLIPDAIASLEMAIQLNPYVGLYHFRLAKLYRQ
                     KNMLTEARKSAEMSINLGTNTEDLLLFLAELFLQEGSYRKGLDYLDDRFSGLPESPEL
                     LSIRGRLLMKTGDPFRAISDLTKASENGNITARKVLAIHYLDKNPLESIKIIGDDSMG
                     DDSLTWILIRAYHKLGRLDTLPNLMELLREPVPDQILSLARMFRDADQYSQAREQYSM
                     LIEQDSTHTTAINELAELERNIAYLQNTKSAVQADTLTQLIQRDSVSNNE"
     gene            11474..13417
                     /gene="thrS"
                     /locus_tag="VGA21_10120"
     CDS             11474..13417
                     /gene="thrS"
                     /locus_tag="VGA21_10120"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009582900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEX9650906.1"
                     /translation="MSEIKITLPDRSVKQMPLGSTALDLAMIISEGLARNVLVTKVNG
                     EIWDAFRPITHNASVKLLTWNDLEGKSAFWHSSAHLMAEALEALYPGIKFGIGPPIEN
                     GFYYDVDMGETEFGEENLKEVEDKMLELARRKNEFSRKEISKVEALKYFKEKGDEYKI
                     ELINELEDGTITFYHQGNFTDLCKGPHIPNTSFIKSVKLLNVAGAYWRGSEKNKMLTR
                     IYGITFPKQKELTEYLAMLEEAKKRDHRKLGKELELFAFSEKVGMGLPLWLPKGTMLR
                     ERLVAFMKDAQTKAGYEQVITPHIGSKDLYVTSGHYEKYGKDSFRPMTTPQEGEEFML
                     KPMNCPHHIEIYKVKPRSYKDLPVRMAEFGTVYRYEQHGELHGLTRVRGFTQDDAHIF
                     CRPDQVKDEFKNVIDLVMLVFKALDFNEFKAQLSFRDLSNPENYLGEVSAWDLAEKQI
                     TEAAEEKGLETVVEIGEAAFYGPKLDFMVKDALGRSWQLGTIQVDYQLPERFELVYIG
                     SDNQKHRPVMIHRAPFGSLERFVAVLIEHCAGNFPLWLTPDQIAVLPISEKFSDYAAE
                     VYNTMAASDIRGFVDNRDEKIGRKIRDAEVKKVPFMLIVGEKEQSGRGVSVRRHGEGD
                     LGYFSIEDFISFFNEKSMLELPN"
     gene            13513..14073
                     /gene="infC"
                     /locus_tag="VGA21_10125"
     CDS             13513..14073
                     /gene="infC"
                     /locus_tag="VGA21_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009185919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEX9650907.1"
                     /translation="MNRPRSFGGFRGRFQEPYRVNERINAREVRVVGENIKVGVYTLN
                     KALEMAGAQGLDLVEISPSADPPVCKIIDYSKFKYEQKKKQKEIKAKTQKTVLKEIRF
                     GPNTDEHDFNFKVKHAINFLGEGAKVKAFVHFVGRTIVFKERGEILLLRFAQALEDYG
                     KVEQMPRLEGKRMFLILSPKVVKRNN"
     gene            14077..14271
                     /gene="rpmI"
                     /locus_tag="VGA21_10130"
     CDS             14077..14271
                     /gene="rpmI"
                     /locus_tag="VGA21_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009582902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEX9650908.1"
                     /translation="MPKVKTKSGAKKRFKLTGTGKIKRKHAYKSHILTKKETKQKRNL
                     TKFAIVHKSDEKRVKKMLNK"
     gene            14346..14690
                     /gene="rplT"
                     /locus_tag="VGA21_10135"
     CDS             14346..14690
                     /gene="rplT"
                     /locus_tag="VGA21_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010610422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEX9650909.1"
                     /translation="MPRSVNNVASRARRKKILKLAKGYFGRKKNVLTVAKNAVEKGLT
                     YAYRDRKTKKREFRYLWIQRINAAVREHNMSYSQFIGALKRAEIDLDRKVLADLAMNH
                     PEAFAEIVSKVK"
     gene            14824..16125
                     /locus_tag="VGA21_10140"
     CDS             14824..16125
                     /locus_tag="VGA21_10140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650910.1"
                     /translation="MKNLVISSFVLLCIFSGEAFSQAGWNWGENEDLAKEKYALYTDA
                     FKNGNFEVSLDPLIWLLTNTPDLNESIYINGAKIYSELADKEADAAKKDVYIAKSLEM
                     YDKRIEYFDGGGDVLNRKATAAYNFYKKDKGKYPVLYSFYEAAFAVDVKDIHTNNLVA
                     YMDVIRLHKGTGGKLTDDEVFEKYEKINEIIDSRSVEDKEKMNKVRELVDRLLISTVK
                     VDCDFVINNLGLKLRNNMQNLKLAKKVMALSLASNCSDDPIFLVAAKIVQKSEPDFGI
                     AKVIGMKSAAEGDFDNALKYYNEAIELSNEPEKKGEIYYEIAIQYLKNGNKPQARAYA
                     YKAVQEDASLKKSYKLIGDLYFNSFNECKEDQSKVQDRAIYLAAYEKYKLAGDQKMMQ
                     SAMEQFPSINDIFELNMEEGQAINVGCWINETVVLQRRPSS"
     gene            complement(16221..17105)
                     /locus_tag="VGA21_10145"
     CDS             complement(16221..17105)
                     /locus_tag="VGA21_10145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650911.1"
                     /translation="MKRHICFFSIFLAACGAQEQVKQELNPESLVGTWERVSYVDSDI
                     SDTAWVDQNPKIIFLKHLTPTHFTWLSFNTETEELNGTGGGTYQYDGTTYTEDIMYFY
                     PTGSSIQGQTIPFTVDFVNGNWHHVGYFQNTEYDPEMVEIIRTDTSLIEEMWKRVPAS
                     EEVSDLVGTWELVSQRKRDDSTRIELPPFMKYYKLVTPTHFNWVQINMEDNEVYAQGG
                     GTFIVDGNTYTERLLYHHPVEEALRGLVIPFNFKMENERWYHRGTFIRPISDKAGNKT
                     GLDTVYIDEVWQRWKPQN"
     gene            17260..18423
                     /locus_tag="VGA21_10150"
     CDS             17260..18423
                     /locus_tag="VGA21_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009195863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX9650912.1"
                     /translation="MGDKLGMGMVGGGIDSFIGEVHRMAARLDNQIELICGALSSTGE
                     RSLQSGKILGLNPERIYRSYHEMINKEVTANYIPSMAFVSIVTPNHLHYQPARLALES
                     GFHVMVDKPLTHSVEEAYNLRQVVKATGKLFGITYTYAGYPMVKEARYLISNDTFGKI
                     RKVNVDYPQGWLTRNIESEKQKQASWRVDPAKSGKGGAMGDIGTHVENIIHYTTGLEI
                     EELCADLSSRYNRKLDDDASILLRFTNGASGSVRASQVLSGEENSLKISVYGENGSLE
                     WSHTDPNSLILKWIDKPTQVYKAGAEKSYLSRAAKNNMRLPSGHPEGFIEAFANIYRN
                     FAYAIKSVEAGKSPDLENFDFPGIEDGLRGMVFIETVVASSNAGCKWKKFEIK"
     gene            18424..19479
                     /locus_tag="VGA21_10155"
     CDS             18424..19479
                     /locus_tag="VGA21_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010610957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEX9650913.1"
                     /translation="MNTIKGPALFLAQFMGDKNPFNSLATICKWAAGIGFKGVQIPSW
                     DSRCIDLTKASESKDYCDELKGTVENEGLVITELSTHLQGQLIAVHPAYDLQFDAFAP
                     DGVKGNPAGRTEWATDQLKKAASASRNLGINVHATFSGALLWPMVYPWPQRTPGLVRD
                     GFEELARRWKPILNYFDKYDVDVCYEVHPNEDIHDGITFELFRECCGDHPRVNILYDP
                     SHFVLQQLDYLQYIDFYHQFIKIFHVKDAEFNPTGKSGVYGGYQEWIDRPGRFRSVGD
                     GQVDFKSIFSKLTRYSYKGWAVLEWECCIKHPEDGAKEGAKFIKDHIIRVTEKSFDDF
                     AGSKSDKSINKKLLGLK"
     gene            19476..20189
                     /locus_tag="VGA21_10160"
     CDS             19476..20189
                     /locus_tag="VGA21_10160"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HEX9650914.1"
                     /translation="MKKIYLVALLCPLSIMACTSKNSEYAVEGPEEWTVLFDGSNLDQ
                     WRGFKKDHVPSVWYIEDSVLICMDSEMRNAEEAETGDIMTREKFQNFELSLEWKISTG
                     GNSGILYFVQETDSSNYSYYTGPEMQVLDNEMAGDNLEKHMAGDLYDLISCSKISVKP
                     VGEWNSVRIVADGSHVEHWLNGVKVIDYDLQSVEMDSLMKNSKWANYPEFGLTTSGHI
                     ALQDHGNRVWFRNVKIRSL"
     gene            complement(20186..21085)
                     /locus_tag="VGA21_10165"
     CDS             complement(20186..21085)
                     /locus_tag="VGA21_10165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650915.1"
                     /translation="MKKIFITGWLFLAVNLIYGQDENRIIFGSLECSPAGDKIIFSAI
                     SVKSDWSNFKPENWVVLQYNLADKSLIEISTGGLYASYSPDGNSIIFEKRTGESQNIF
                     VRDLDSGNEFQLTDNEHRNFGGSWSPDGQQIVYNSNQTGTIEIFICNKDGTNHRQLTF
                     SGEHKSYNPKWSPFGNDIVYYFEKGDGKDQIYVMNSNGEKVRNITNNDSHSYYPSWRD
                     EKSVVFAADPDEIHTISLDGSKNQPILGIKSFYAVFSRDGSKLFFINTADKDRITINI
                     MEMKSSKTSILLAGEDLEELVKN"
     gene            complement(21154..21927)
                     /locus_tag="VGA21_10170"
     CDS             complement(21154..21927)
                     /locus_tag="VGA21_10170"
                     /inference="COORDINATES: protein motif:HMM:NF014590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HEX9650916.1"
                     /translation="MTSFFLEKQTYYYLLTGWILIALLSYPFLRRKTQPYGRHTTSKW
                     GPLIDNKIGWILMELPALIVAPALYIIGNGEKNLPMDFFMFLWVFHYVNRALVFPLRT
                     KTSGKKIPVAIVASAIIFNCINGFFNGYYFGFLSPFYESHWYYDGRFLIGLFFFIAGL
                     AINWTADNQLIRIRRNSSDGYKIPYGGLFEYVSCPNHFGEILEWFGFAILTWSLPAFS
                     FSIWTFVNLIPRSKNHHRWYKEQFPDYPVERRAVIPFVT"
BASE COUNT         7149 a         3837 c         4692 g         6273 t
ORIGIN      
        1 accagaaatt tctgcgtcct ttggtacaag tttgtttcgt caggatttcc caatagtttg
       61 attatttctt cttccgaaag tcccattagt tttgtccgct gttccattag tgatgaaatc
      121 tctaccttcc ttaatgactt gcatccctgt tcatcatttt tccatttttc catgttgaaa
      181 ttatctaaag cgggtattgg agaacaagca ataataagaa taagaaacgc tggaattctt
      241 ttcatacttg ttgaaccttg gtactgatga taagtcaaag ccggactgat gagattgcaa
      301 aataaagaat tcctgagatt aaccagttca cattccaatc atttataaaa gaaatgaata
      361 ttaaagatgt tgaaaaaata atgatccggg ttagttccag ggatttgatc cattttttag
      421 attcaaataa agctccacaa tttattatcg taataattat ggtggatact attacggatt
      481 tttgcaaaag agtgaatatg ttctggttga ataaaaatac gggttacagt gcattctcta
      541 ctaagaacca accattgtta aagaatatcg tacattttct tatattgtaa gcagatgata
      601 aacgaagaac aaaaaaatat cattatagaa gttttgcagc cttactaccc taaaatgatt
      661 ggtgtttttg gctcctttgc ccgaaatagg aacaatcatg atagtgacct ggatattctt
      721 gtcgaatttc aggatacagt aaatcttctg gataatcatc ggagttgaaa ttaaattatc
      781 tgaattacta aatctggaag tcgatcttgt aaccaaaaaa gctatctccc cactgttagc
      841 ataccatatt gataaagatc tgctgaaaat cttccatgaa gaaggataat tcaatatacc
      901 tacaccatat caatgattgt ataaccaaaa ttgatactta tacaacagac ctttcccagg
      961 gtaatttttt gagtaattct ctgttcacag gatgctgtta tcaggaattt tgaaattatc
     1021 ggtgaagcaa ccaaaaaact ctcaaggaag gcaaatcgat aaaatgaaat tgtttgttta
     1081 cctttaaaaa tctcaaaatt tatacgtgtg aataaattca gcaaaccctt ctggctgctc
     1141 tctttttttc taacttcagt catatttatt tacacctatg caaacatgga tgacatggtt
     1201 agctactacc aaaatcccta tactttgtca aagctttaca tatcacggga attattcttt
     1261 ttcattggcc tggcaaccat aatagtaacg aatcttgcct tgtatgtctt ttccaaattg
     1321 atacgcgact cacatgtagc aaaggagtat tttaaggaat taggaacctg gtttattacc
     1381 atggccggta tctcgaatat ctttcttttt gtctcgctgg tgtttatcct gctattcaac
     1441 aggctggatg attttgatcc ccgggtgttg ggcttcttcc tttactttat tggttttatc
     1501 gcttttgcgt ggcttgtcag actaattatt ctccttctca agaggcagca atgatgtaag
     1561 aatatcttga ataaatataa taaatcagct ctttaaacca ccactttact ttacttttct
     1621 aatcttcaca tctttaataa gttatacttt taaacttaaa gaacttaatt agtttttcct
     1681 gcgaaattct gagtcgtgta catttaccgg aattctttaa aaagttaaaa atttgaccaa
     1741 tttcaaacat tttcgatgca aatagatttc ctttgcacag ctaggattta cacctccgaa
     1801 tgtattaatt tagaaacgat taaagtcaag ccaatcttaa aaaaattcat tttttatggc
     1861 ccctggaaat tatacagaag ctagtattaa gtctctggac tggaaagagc atatccgctt
     1921 gcgaccgggt atgtatatag gaaagctggg cgacggctct tcccctgatg atggcattta
     1981 tgttcttgta aaagaaatcg ttgataatgc cattgatgaa tacgtgatgg ggtttggccg
     2041 gacaattgac attaaaatct ctgaccagga agtagaagta agagactatg gtcgtggaat
     2101 tccattggga aaggtgattg attgcgtttc aaaaataaat accggtggaa aatacgactc
     2161 agaagccttt cagaaatctg ttggattaaa tggtgtaggc acaaaggcag tcaatgcatt
     2221 atgcaaaaac tttcttgtgc aatccttcag ggagggaaaa actaaagtag cagaatttgt
     2281 gcggggtgag atcactaaaa atccaaagat cgctgccaat aaatccaaaa gtggaaccct
     2341 ggttgtcttt aaacctgatg gaaacatttt taaaaacttt cactatatac ccgaatattt
     2401 agaagaacag atttggaatt atgtattcct gaattccggc ctgacgatta atctcaacgg
     2461 caagaaattc cattcccaaa atggacttct cgatctgctt aaaagaaaga ctgatgaaga
     2521 cagtctgaga tatccgataa ttcaccggaa agggaaggat attgaatttg caatttctca
     2581 ttctaatcaa tatggggagg attactactc ctttgtaaac gggcagttta caactcaggg
     2641 tggaactcat ttggctgcct ttcgggaagc tattgtaaaa acaatccgtg agttctataa
     2701 aaaagattac gattcaacgg atatccgggc aagcataatt ggggcaattg caatccgtgt
     2761 tcaggaacct gtttttgaat ctcagactaa aacaaagttg ggttcacaaa atatcgcgcc
     2821 agaaggtcct actgtgcgga cttttattaa tgattttgta ggaaaagaat tagataactt
     2881 cctgcaccaa tctcctaaag ttgctgacgc tttgttgaag cgaatacagc aatctgaacg
     2941 cgaaagaaag gaaatagcag gtattaaaaa acttgccaat gaacgagcca agaaagcaaa
     3001 tcttcataat aagaaactta gagattgcag acttcattat gatgaagata agtccggggc
     3061 cgaaacaatg atttttatca ccgaaggtga ctctgcaagc ggttccatta ccaaatccag
     3121 agatgtccag acgcaggctg tctttagctt aagaggtaag cctctgaatt cctatggact
     3181 caccaaaaaa gtggtatatg aaaatgaaga gttcaacctg ctgcagcatg cactaaatat
     3241 tgaagatgga ttggatggcc tgcgctacaa gaaaattatt attgcaactg acgcggatgt
     3301 ggatggaatg catatcagac tcttattgtt aactttcttc ctgcaatttt ttccggacct
     3361 ggtgaagcat ggtcatgttt tcattcttga aaccccttta ttcagggtaa gaaataagaa
     3421 agaaacaatt tattgttatt ccgaaggaga caaagagact gcggtaaata aattgggtgg
     3481 aaaacctgag ataacccgct tcaaaggttt aggagaaatt tctccggatg aattcaagaa
     3541 tttcatcgga gaaaagattc gtttggaacc ggtgaaggtt ggcagagatt caacagttaa
     3601 acaaatactg gagttctata tgggtaaaaa tacacccgac aggcaagagt ttattataaa
     3661 aaatttaaaa gttgaaaaag atattgttaa ggaagaagtg gttgtagctt aagatggcaa
     3721 aaagatccag aaagaaaaaa tcaccggacc gggaagagga tttggtccat gatttaagac
     3781 ccgtctcagg gttatataaa aattggtttc tcgattacgc ctcctatgta attcttgaaa
     3841 gagctgttcc tgcgatagaa gatggtttca agcccgtgca gcggcgtata atgcatgctc
     3901 ttaaggagat ggatgatggc cggtttaata aagtagccaa cgtaatcggt agtactatgc
     3961 aatatcatcc gcatggagat gcctccatcg gtgacgctat tgtaaatatt ggacaaaaag
     4021 aattactttt tgatatccag ggaaactggg gtgatgtgag aacaggtgac agtgccgcgg
     4081 cagcacggta tatcgaagtt aggctttcag aattcgccaa agaagtagtt tataacaacc
     4141 agaccaccca atggcagctt tcttatgatg gccgcaaaag ggaaccagtt accttgccgg
     4201 tgaaatttcc acttttgctt gctgccggag ttgagggtat tgccgttggc ttatctacta
     4261 aaatccttcc ccacaacttt cgtgaactga ttaaagcaag tattgaggta ctgaagggta
     4321 agagccctaa aattttcccc gactttccaa ccggtggaac ggccgacttc tccgaataca
     4381 atcagggtta taaaggaagc cgggttcgaa tcagagccaa gattgaagct attgataaaa
     4441 aaaccctgat aataaaggat ataccttttg gtacaactac aaccggcttg attgattcca
     4501 tcattaaagc aaacgacaaa ggtaaaatca aggtgaagca ggttattgat aatactgccg
     4561 aacacgtgga aattctggtt catctggccg ccggccagtc tcccgatgtt gcaatcgatg
     4621 ctttatacgc atttacagat tgcgagtatt ctgtatcacc gaatgcctgc gtgattattg
     4681 atgagaagcc gagatttctt catgttaacg agattttgaa aataaacacg gagaaaacgg
     4741 ttgctctttt gaaacaagag ctgcaaataa ggaaagaaga acttcaggag cgtattcttt
     4801 tttcttctct cgaaaagatt tttattgaaa agagaatcta caggaatatt gaagagtgcg
     4861 agacatggga tgccgttatt aagacaatag ataaaggact aaagccgtat aagaagcaat
     4921 tttaccgtga aattaccggg gaggatattg ttaagcttac cgagataaaa attaagcgga
     4981 tttccaggtt tgattccttt aaggctgatg agttaatgaa aaagctggag gaagagttga
     5041 aggaaaccct atacaatctg aaaaacctca cgaatttttc catcaggtat tatgagaatc
     5101 tcctggaaaa atttggaaag ggaagggagc gaaaaacgga gtctaaatct tttgacgtaa
     5161 ttactgcaac ggttgtggcg gctacgaacc aaaagcttta tgtgaaccga aaggatggct
     5221 tcgccgggta tggtttaaga aaggatgaat ttgtgagtga atgttcggat ctggatgata
     5281 taatcgcgat gaggtcggat gcaaaattca tggtttcccg aattagtgag aaaacatata
     5341 tggggaaaga aattatgcag gtggccgtct tcaaaaagaa tgatgaacga atggtctata
     5401 acgcggtcta cgtggatggg aaaacgggaa aatcaatggt caagcggttt caggttattg
     5461 gcgttactcg tgataaagaa tatgatattt ccaaaggagc gccgggaagc aaactgcttt
     5521 acatttcagc aaatccgaat ggtgaagcgg aagttataac cgtgaaactc actcagggaa
     5581 gcaaagccag gaaaaaagtt ttcgatttca acttttcaca actcgatata aaaggtcgcg
     5641 gtgctggggg aaatattatt accaggtacc cggtcaggaa aattgaacgt aaaactaccg
     5701 gggtaagcac attgaagggg ctggatattt ggtatgatga atccgttggc agactaaaca
     5761 gggaagggcg gggtaaattg gtgggcaatt ttgatggtga tgatcggata attgttttct
     5821 acaaggatgg tcactatgaa ataaccagct atgaactctc caacagatat gaatatgata
     5881 aggtggtgga agtcgcaaaa cttagaccag atgttattat tacggcagtt cattacgatg
     5941 gaggcagtaa aaattattac gtaaagcgtt ttactgtaga aacttctact ctcgataaaa
     6001 tgttccggtt cattagcgag gaaccgggaa gtaaactggt cctggcaacc acgaacccaa
     6061 atcctgttgc aatcctggaa attgaaaaag gaaagggaaa aaccagggag gctattcaaa
     6121 tagatcttac agaagtagtc gatataaaag gctggaaagc ttttgggaac agactttcaa
     6181 actacaaaat caaaaacgtt aagcttgcgg agacggtgaa atcaggggcc gagtttatgg
     6241 agaaaccaga agaaaaacca aagctgaaaa aacaaaagtt gcaaaagaag gtaaagacca
     6301 aatctgtcaa atccaaaacc ttttctaaaa aagccaaaac caacgcttct aaagagaaat
     6361 caattcccgg gaaacctgta atcaggaaat caaaatcttc aaaaaaaaaa ggaaaaataa
     6421 ataatcaaca agatttgttc gcctctcaaa attcggagtc ttttgaagtt ggatctacta
     6481 ttcaacttga cttgtaatgg accaggaaaa taataaacgg ctgcttgatt ttatttctcc
     6541 ttttgtaacc attcacaaaa gggaaagaat tgaagaggtt ttaactcaaa gaacaagaca
     6601 tctcactgtg gttctcgaag atatttatca gtcacagaat gccagcgctg taattcgttc
     6661 ttgtgaatgt attgggattc aggatgttca tgtaattgaa caaaaaaatt tctataaagt
     6721 gaatcgtgat gtattgcgag gtgccggtaa atggatttca ctgcaccggt ataaaactgc
     6781 aggttccgcg caggattgtt tttctacttt gagggaaatg ggatacaaaa ttattggtgc
     6841 agcaccggaa actatttact ctacaattca agaaattgac ataaatcata aattggcaat
     6901 tgtttttggt acagaattgg aaggtttaag tgattatgct cttaaaaatg tagatggaat
     6961 ggtaaggatt ccaatgtccg ggttcacaga aagcttcaat ttatcagtaa gtgccgccct
     7021 ggtgaccttt aacttgttga cgaaagtgaa gactgaattg caaaattggg aacttactga
     7081 aagagaaaaa ctcgaactaa gattagaatg gtataaaaag tcaattaaaa acgcggtaaa
     7141 actggagact gaattttttc gtttataccc ggggatatca tgaaagttgt gaagaaaata
     7201 ttgaagtttt tacttgtgat ttttgcggta atattcgttt ttatcctggc gtccaacttc
     7261 tggatttgga atgttaccca tcacagagtt tttgaaagca ttgatgacct ccctgagaac
     7321 gaagtggccc ttgttcttgg cactagcaga tttaccgtgg gaggaaaagt gaatcccttt
     7381 tttaaataca ggattcagtt ggcagctgaa ctgtataaaa gtggaaaggt aaaatatatc
     7441 attgtgagtg gagataataa cacgaagtac tacaatgagc cgaaagaaat gcgtgacgcg
     7501 cttcttcttt taggaatacc ttatcgcaaa ataattcttg actatgcggg attcaggact
     7561 cttgattcaa tggtcaggtg taaagaaata tttggccaga aaaaaataac cattattact
     7621 caaaaatttc atagcttcag agctattttc attggggatt attatggaat ggagaccacg
     7681 gcaatcgcgg caagaaattt acccttccgg caatcattta aagtggaatt gcgtgagacc
     7741 ttggcaaggt ctaaagcggt aattgatttg tacatcctcc gggagagccc caaatttcta
     7801 ggtgagaagg aaatcatagg ccaggtcagg taaacaattg taaaaaataa taagagtcgt
     7861 aattatgagg gaattaaaaa tgttccatac ttgttataat tttatcttat ggccaaaaga
     7921 acttttgtaa aagttttttt tactcttctg tttattattg ccttcaccag gatatttggg
     7981 caggaaacta taattaaagg cagaataact gatgccaaat ccggcgatcc cattcctttt
     8041 tgcaatattt ttttccaggg tacaaatatt ggaactactt cagattttgt tggtttttat
     8101 accatcagga catccacacc taaggattct ctggtggctt cctatattgg ttataaacag
     8161 cgattaaaac cggtaaaatt tgctgtagtt caggaaataa attttcaact ggctgaagat
     8221 attcaacaac ttaaagaact ggtatttgtt gcggaagaaa atcctgcctt tgccatcctt
     8281 agaaatgtag ttagaaataa aaatctgaat gataaaagat cgcttgattc atacgaatat
     8341 gaaagctata ataaaataga attagacctg gataacctga cagacaaatt ccgtgaaaga
     8401 aacattgtca agaaagtgaa gaaagtgatt gatagtgtgg accagatagc cggtgatgat
     8461 ggcaggccaa tattaccttt ttttatcagt gaatcaatct cgaaagccta ttacaggaaa
     8521 actcctgagc aaaagaagga acatataatc aaaacaaaaa tctccggagt gggagttgaa
     8581 gatggaactt taatttccca ggtaattgga agtacatttc aagagtacaa cttctaccag
     8641 aactggctaa acatctggga taaggaattt gtatctccaa tcgctgatgg ttggcgggct
     8701 tactatgatg tgtatttgat ggatagcgtt atgattggaa attttttctg ttacaggctt
     8761 gaagtgtttc cgaaaagagc aggtgacctt gcctttacag gcagcatctg gatcgacaaa
     8821 aatacctggg ctctgaagca ggctgattta aggatagata aaattgcaaa cctcaactat
     8881 attgaaaaac taaagattca acaggaactg gttcctactt cttccggtgc ctggattgcc
     8941 aacaaatcac gagtatccac tgactttcag tttctgggtg agtttggaat atccaatcag
     9001 gcagccggaa ttctcgcgaa gttctacact tcaaataaag acatcgtggt taaccaggaa
     9061 tacaaaagga atttctttga ccaggctata acgatggaag aagatgtggc ggagtttgaa
     9121 gataattatt ggcctgaaaa ccggcatgac ccgctcactg ttgcagaaac aaatgttatt
     9181 caaatgattg ataccctcgc ggaaattccc acaatacgga gattatcaaa tacgattacc
     9241 tatttgtcaa ccgggtatat tgataaactt tggatttttg aacttggacc atttccctta
     9301 ttttttgcat ccaacaacat agaaaaattt cggttccggc tgggtggtat tacttctatc
     9361 aggttcagca gaaagtttgt atttagcgga tacggagcct atggaattcg tgatgataaa
     9421 tggaaatatg gagtaggatt ggattttatc attgacagga aaccctggac taaaataggg
     9481 atttccagaa cctatgatat ccagcaagtc ggtttaactt ttgatgatgt cttttcaaac
     9541 ataggcctga taacttttga atcctatttc agaaacattg gacatgaaat gccattcttt
     9601 cttaccgaga ataaaataaa ttttcaaact gacttaaaaa aaggaattac tcaaaaactc
     9661 gaagtcaggc aaagagatta taatcccatt aatctggata caacctggaa ttacagctac
     9721 aggataaacc cggttgaggg aggagcttca ccattacgca gtaatttctc tacctcagaa
     9781 attagttttg aaagccgttg ggggaaggat gaaatttggt tgattgatga caataaaagg
     9841 ttctcattgg gtcctgttaa gtttccggtt atcactttac ggtacacatt gggtataaaa
     9901 gatttcatgg gtggtgattt taattaccag aaagttgccg taaatatcta caagccggta
     9961 aagatgggaa tattgggcat ctctaatttc aatataaccg gttcaaaaat ttttggagcg
    10021 gtaccattac ctcttcttta tggtcacctt ggaaatgagt caccttttta tgtaagcaca
    10081 gcattctcaa ccatgggttt ttctgaattt tttagcgacc aatttatttc gctcggctac
    10141 cggcatcatt tcgaaggatt cgttttaaat accattccct tatttaagag gctcaagtgg
    10201 aggttagtag ccaacgcgaa tattcttcag ggaaatgttt ctgcaaaaaa cgaggatatt
    10261 cattctgatg taggaggaaa ccagttgctc ccaactcagc cgcttcgatc tctgagctca
    10321 aaaccctacg tagagttggg atatggaatt gaaaatatct tcagagtttt caggattgat
    10381 gcttttcacc ggcttacata cctggagaac agaacaggaa gtgcatttgc tttgaagttc
    10441 agtcttcaat tcgttttcta aggtttctat taccttaatt tcaattactt ttgctgcaca
    10501 atggtatgga attcaactct tttaagtacg ctgctaattg gttttttctt attgtcttgc
    10561 cattctcccc ggaatgaaaa agtaccagac tcaaaacctt tatcctcaga agtgtataaa
    10621 aagcaactct cagagttgga agaaccggaa ggaatgcttg aaaaagaaaa attctttgca
    10681 aaaaaagcat ttcttgaaga acaatctgga ttgatccccg atgcaattgc cagcctggag
    10741 atggccatcc aacttaatcc atacgtaggc ctttatcatt ttcgcctggc aaaattatat
    10801 agacaaaaga atatgctgac agaagcgcgc aaaagtgctg aaatgtcaat aaatctgggt
    10861 actaataccg aagacttatt acttttcctt gcagaattgt ttcttcagga agggagctac
    10921 aggaaagggt tagactattt ggatgaccgg ttcagcggat tgccggaatc tcctgaatta
    10981 ttgtccatca ggggaagatt attaatgaaa accggagatc cattcagggc aatttctgat
    11041 ttgaccaagg catcggaaaa tggaaacata acagctagaa aagttcttgc aattcattat
    11101 ctcgataaaa atccccttga atccatcaaa ataattggag atgattccat gggcgatgac
    11161 agtctcacct ggattttgat tagagcctac cataaactcg gacgattgga tactctccca
    11221 aatctaatgg agttgcttcg cgaacctgtt ccggatcaaa tactttcgct ggcaagaatg
    11281 ttccgtgatg cagaccaata ttcccaggca cgagaacaat atagtatgtt aattgagcaa
    11341 gacagcaccc atacaactgc aataaacgaa ttggccgaac ttgaaagaaa tattgcatat
    11401 ttgcaaaata cgaaaagtgc tgtacaagca gatactttga cacaattgat acaaagagac
    11461 tcagtttcta acaatgagtg agataaaaat aacgttgccg gatagatcag taaagcaaat
    11521 gcccttggga tccaccgcac tggatttggc tatgatcata agtgaggggc ttgccaggaa
    11581 tgtgctggtg acaaaagtca atggtgaaat ttgggatgct ttcaggccaa ttacccacaa
    11641 tgcatctgtt aagttgctca cctggaatga cctcgaaggg aagtcagctt tttggcattc
    11701 ctcggcacac cttatggctg aagcgctcga agcattatac ccaggcatca agttcggtat
    11761 tggaccgcca attgaaaatg gattttacta cgatgttgac atgggcgaga ctgaatttgg
    11821 tgaagagaat ctaaaagagg ttgaagataa gatgcttgaa ctggctcgcc ggaaaaatga
    11881 attttcacga aaggaaatca gcaaggtgga agcactgaaa tattttaagg agaaagggga
    11941 tgagtataag attgaattaa taaacgagct ggaagatggt acaattacat tttatcatca
    12001 gggtaatttt acagatttat gcaaggggcc tcatattcca aatacatcat ttatcaaatc
    12061 agttaagttg cttaacgtag cgggtgctta ctggagggga agcgaaaaaa ataaaatgct
    12121 cacccggatt tatggaataa catttcctaa gcaaaaagaa ctcacggagt acctggcaat
    12181 gctggaggaa gctaaaaaga gagaccatcg taagttggga aaggagcttg aactttttgc
    12241 attttccgaa aaagtaggaa tgggattgcc actttggctg ccgaaaggaa ctatgctaag
    12301 agaaagactc gtagccttta tgaaagatgc tcaaacaaaa gccggatatg agcaggttat
    12361 tactcctcac attggaagca aagatcttta tgtcacttcc ggacactatg aaaaatatgg
    12421 gaaagattca ttcaggccta tgacaactcc ccaggaagga gaggagttca tgctaaaacc
    12481 catgaactgt ccccatcata ttgaaatcta caaagtgaaa cctcgctcat acaaggatct
    12541 tccagtcaga atggctgagt tcggaacggt ttacagatat gagcagcatg gcgaacttca
    12601 tggattaacc agggtaagag gatttactca ggatgatgcg cacatttttt gcaggccaga
    12661 tcaggtaaaa gatgaattta agaatgtaat agatcttgta atgttggtat ttaaagctct
    12721 ggattttaat gagttcaaag cccaactttc atttagagat ttaagtaacc ctgaaaatta
    12781 tcttggagag gttagtgcct gggatttggc agaaaagcaa attacagaag cagctgagga
    12841 aaaaggtctg gaaaccgttg ttgagatcgg tgaagctgcc ttttatggac ccaaactaga
    12901 cttcatggtt aaagatgccc tgggacgcag ctggcaattg ggcacgatcc aggtagatta
    12961 tcaattgcct gagcgttttg agttggtata catcggttca gacaaccaga aacatcgacc
    13021 cgttatgatt caccgggctc catttgggtc tctggagcga tttgttgctg ttctgattga
    13081 gcattgcgct ggtaattttc cattatggct tactcccgac caaattgccg ttcttccaat
    13141 ttcagagaag ttctctgact acgctgcaga ggtttacaac acaatggccg ctagtgacat
    13201 taggggattt gtggacaacc gggatgaaaa aatagggcga aagatcagag atgcggaagt
    13261 taagaaagta ccctttatgt taattgttgg tgagaaagaa caatcaggca ggggagtatc
    13321 tgttaggagg cacggggaag gtgatttggg ttacttttcc attgaagatt tcatttcatt
    13381 tttcaatgaa aaatcaatgc tggaactgcc caattagccg tttttcatgc ggttaatata
    13441 tatgcccact cttttgattt aatgataact atatcgatat ttgcacttca attttaaaaa
    13501 aaggaggaaa atattaatag gccgaggagc ttcggtggtt tccggggaag atttcaggaa
    13561 ccgtatcgag ttaacgaaag aataaacgcc agagaagtaa gggttgttgg ggaaaatata
    13621 aaagtgggcg tatacacatt gaataaggcg ctggagatgg caggcgcaca ggggctggac
    13681 ctagtggaga tttcaccatc cgcagaccct ccggtttgta aaataataga ttactccaag
    13741 ttcaaatacg aacagaagaa gaaacagaaa gagataaagg ctaaaaccca gaaaactgtt
    13801 cttaaggaaa tcaggtttgg ccctaataca gatgaacacg attttaactt taaagttaag
    13861 cacgcgataa atttcttggg tgaaggcgcc aaggtgaaag ctttcgttca tttcgtcggg
    13921 agaacaatag tgtttaaaga gaggggagaa attttattac ttagatttgc ccaggcattg
    13981 gaagattatg gtaaagtgga acaaatgccc cgcctggaag gtaaaagaat gtttttgata
    14041 ttatctccca aagttgtaaa aagaaataat tagaaaatgc caaaggtgaa aacaaaatcc
    14101 ggtgccaaga agaggtttaa actaaccggt acaggtaaga taaagagaaa acatgcttat
    14161 aagagtcata ttttgaccaa gaaagaaact aagcaaaaaa gaaatctcac aaaattcgct
    14221 atcgttcaca aatcagacga aaagcgggta aagaagatgc taaacaaata aaggtttatg
    14281 tttaacccgg gttaatggct caaagaactc ctgttggaga cgccttaatc ccaaaaatca
    14341 aataaatgcc cagatcagta aataacgtcg cctccagagc gcgaagaaag aaaatcctta
    14401 aactagccaa aggttatttt ggaaggaaaa agaatgtctt aaccgttgca aagaatgcgg
    14461 tagaaaaagg ccttacttac gcttacagag atagaaaaac caagaaaagg gaattccggt
    14521 atctttggat acaacgaatc aacgctgcag taagagaaca taacatgtcc tattctcagt
    14581 ttattggagc ccttaaaaga gctgaaattg acttagatag gaaagtactg gccgatttgg
    14641 ccatgaatca tccggaagcc tttgctgaaa ttgttagcaa ggtaaagtaa tttctggcag
    14701 gttttttggc tattatcatt aattcctccc caaaacaggg gggatttatg ttctttaatg
    14761 gaggacaact ttaataattt agtatttttg ttaaatgtta aatttccagt aatctgttca
    14821 aagatgaaaa accttgtaat tagtagtttt gttttactgt gtattttttc aggagaggct
    14881 ttttcccagg caggttggaa ttggggagaa aatgaagacc ttgctaaaga aaaatatgcc
    14941 ttgtataccg atgccttcaa aaacggaaat tttgaagttt cattggatcc gctcatttgg
    15001 cttctcacaa atacacctga tttaaatgaa tccatataca tcaacggagc aaaaatttac
    15061 agtgaattgg ctgacaaaga agctgatgct gcaaagaagg atgtttatat agcgaaatcc
    15121 ctggagatgt acgacaaaag gattgaatat tttgatggag gaggagatgt tctgaacaga
    15181 aaagccactg cagcgtacaa cttctacaaa aaagataaag ggaaataccc ggtgctgtat
    15241 tctttttacg aagcggcatt cgcggtggat gtaaaagata ttcatactaa taacctggta
    15301 gcctatatgg atgttatcag gcttcacaaa ggtaccgggg gaaaattaac cgatgacgaa
    15361 gtttttgaaa agtatgaaaa gatcaatgaa ataattgatt ccagaagtgt ggaggataaa
    15421 gaaaagatga acaaagtgag ggagttggtt gacaggcttc tgatttccac agtaaaggtt
    15481 gattgcgact ttgtgataaa taacctgggt ttaaaactcc ggaataacat gcaaaatcta
    15541 aagcttgcca agaaagtaat ggcattgtca ctagccagta attgtagtga tgatcctatt
    15601 ttcctggtgg cagcaaaaat tgttcagaag agcgaaccgg atttcggaat agcaaaagta
    15661 attggaatga agtctgcagc agagggtgat tttgataatg ccctgaagta ctataacgag
    15721 gcaatagaac taagcaacga acctgagaaa aaaggtgaaa tttattacga aattgcgata
    15781 caatatttga aaaacggcaa caagccgcaa gctagagctt acgcgtataa ggcagttcag
    15841 gaagatgctt ctttgaagaa atcttataaa ctaattggag atttgtactt taattccttt
    15901 aatgaatgta aggaagatca aagtaaagtt caggacaggg caatttatct ggcagcttat
    15961 gaaaaataca aattagccgg agatcaaaaa atgatgcaat ccgccatgga gcagtttcca
    16021 tccattaatg atatttttga actaaatatg gaagagggcc aggccattaa tgtcgggtgc
    16081 tggattaatg aaacagtagt cctgcagaga agaccatcca gctaatcaaa gtaaatattt
    16141 aagtagaaga aggcgactta tgtcgttttt tttcgcaaaa acaggcacgt cttttagtgc
    16201 ttgaaggttt attgatattt tcaattctga ggtttccacc tctgccagac ttcatcaata
    16261 taaaccgtgt caagtcccgt tttattgccg gccttatcgc tgatgggccg gataaaagta
    16321 cccctatgat accatctttc attttccatt ttaaaattaa atggaattac caggccccga
    16381 agtgcttctt ctacgggatg atgatataaa agtctctcag tatacgtgtt gccatccact
    16441 ataaaagttc cgccaccttg agcgtaaacc tcgttgtctt ccatattgat ttgcacccaa
    16501 ttgaaatgag ttggagtaac cagcttataa tatttcatga atggaggaag ttctatccgg
    16561 gtagaatcat ctctttttct ttgactcact aattcccagg ttcctaccaa atcactaact
    16621 tcctccgagg caggtactct tttccacatt tcttcaatca acgaagtatc tgttctgata
    16681 atttcaacca tttcaggatc atattcggta ttctgaaagt accccacgtg atgccaattt
    16741 ccatttacaa aatcaacggt gaaaggaatc gtctggccct ggatgctgga gcctgttggg
    16801 tagaaataca taatatcttc tgtataagta gttccatcat attgataagt tcctccgccc
    16861 gttccattta attcctcggt ttcagtatta aatgacagcc aggtaaaatg agtaggagtc
    16921 agatgtttca aaaaaatgat ctttggattc tgatcaaccc atgctgtatc gcttatgtca
    16981 gaatctacat aacttactct ttcccaggtt ccaactaaac tttcggggtt caattcctgc
    17041 ttcacctgtt cctgggctcc gcatgcggcc agaaaaatcg aaaaaaagca aatatgtctt
    17101 ttcatagcga ttgttgtttt ggtaagttta gtccttataa tatagtgttt ttgaatgacc
    17161 ttgtcaagaa tgaaaatcat tataaaatca tatccagaat ctggaaactt aaagcacaat
    17221 gattatcttt tgtctcaaat aatctaattg aacataatta tgggagacaa gcttggcatg
    17281 ggaatggtgg gaggaggaat tgattctttc attggtgaag ttcacagaat ggccgccagg
    17341 cttgataacc agatcgaatt gatttgtgga gcattaagca gcactggaga acggtccctt
    17401 caatctggaa aaatacttgg attaaatcct gaaaggatat atagatctta ccatgaaatg
    17461 attaataaag aggtaacagc caattatatt ccatctatgg cttttgtttc tattgtaact
    17521 cctaatcatc ttcattatca gccggcgaga ttagcgttag aaagcggctt tcatgtaatg
    17581 gtagataaac cattgacaca ttcagtggaa gaagcctata acttacggca agtagttaaa
    17641 gcaacaggaa aacttttcgg gattacttat acgtatgccg gttatccaat ggtgaaggag
    17701 gcgcggtatc tgattagcaa tgatactttt gggaaaatca gaaaagtgaa tgtcgattat
    17761 ccgcaaggat ggctgacacg aaatatagaa agcgagaaac aaaagcaggc aagctggcgt
    17821 gtagacccgg ctaaatccgg aaaaggagga gctatgggtg acattggtac gcatgtggaa
    17881 aatattatcc attatacaac cggacttgaa attgaagaac tatgcgcgga tctaagctcc
    17941 cggtacaatc gcaagttaga tgatgatgct tccatattac ttaggtttac taatggcgcc
    18001 agtggctctg tcagggcgag ccaggttttg agtggtgaag aaaatagttt aaaaatttca
    18061 gtatatggtg aaaacggttc attggaatgg tcgcatacag atccaaattc cttgatctta
    18121 aaatggatcg acaagccaac ccaagtgtat aaagccggag cggagaagag ctacttatcc
    18181 agggctgcaa agaataatat gcgattgcct tctggtcatc cggaagggtt tattgaggcc
    18241 ttcgcaaata tttaccggaa ttttgcatac gcgatcaagt ctgtagaagc gggcaaatcg
    18301 ccggatttgg aaaattttga tttcccggga atcgaagacg gtttgagagg aatggtattt
    18361 attgaaacgg tcgttgcctc atcgaatgct ggatgcaagt ggaaaaaatt tgaaataaaa
    18421 taaatgaaca caattaaagg accagcctta ttcctggctc aatttatggg cgacaaaaat
    18481 ccctttaata gccttgcaac catatgcaaa tgggctgccg ggataggatt taaaggcgta
    18541 caaataccga gctgggattc cagatgtatt gacctcacta aagcttcaga gagtaaggat
    18601 tactgtgacg aattaaaagg tacagtggaa aatgaggggc tggtaatcac cgaattatca
    18661 acccatttgc aaggtcaact tattgctgtt caccctgctt atgatcttca atttgatgct
    18721 tttgcacccg atggagttaa aggaaatcct gccgggcgca ccgaatgggc aactgatcaa
    18781 cttaaaaaag cagcttccgc aagccgcaat ttgggaataa atgttcatgc aacattttcc
    18841 ggagccttat tatggccgat ggtttatccc tggcctcaaa gaactcccgg cctcgtgcga
    18901 gatgggtttg aagaattagc ccggagatgg aaaccaatcc tgaattattt tgataagtat
    18961 gacgtagatg tttgttatga agtccaccct aatgaggata tccatgatgg gataaccttt
    19021 gaactttttc gggaatgttg cggcgatcat ccgagagtta atattctcta cgatcccagt
    19081 cattttgttt tgcagcaact tgattattta cagtatatag atttttatca tcagtttatt
    19141 aagatatttc atgttaagga tgcagaattc aatccaacgg gaaagtcggg agtatatgga
    19201 ggttatcagg aatggattga caggccaggc aggtttcgtt ccgttggcga tggtcaggta
    19261 gattttaaga gtatttttag taaattaacc cggtatagct acaagggatg ggctgttttg
    19321 gagtgggaat gctgtatcaa gcatcctgaa gatggtgcca aagaaggggc caaatttatt
    19381 aaagatcata taataagagt tactgaaaaa tcttttgatg attttgcggg ttcaaagagt
    19441 gataaatcaa ttaataaaaa actgcttggc ttaaaatgaa aaaaatctac ttagtcgcat
    19501 tattatgccc attgtccata atggcatgta ccagtaaaaa tagtgaatat gctgttgaag
    19561 gtcctgaaga gtggactgtt ttgtttgatg gctccaacct ggatcaatgg cggggtttca
    19621 agaaggatca tgttccatca gtttggtata ttgaagacag cgtacttatt tgtatggatt
    19681 ctgaaatgcg aaatgctgaa gaagccgaaa ccggagatat tatgaccagg gaaaaatttc
    19741 aaaattttga attatctctt gaatggaaaa ttagcacagg agggaacagt ggcatcttat
    19801 attttgtgca ggagactgat tcaagtaatt actcctatta cacaggtcct gaaatgcaag
    19861 tgcttgataa tgaaatggcc ggtgataacc tggaaaagca catggccgga gatttatacg
    19921 acttgattag ttgcagcaaa atttcggtaa aaccggttgg tgagtggaat tctgttagga
    19981 ttgtggcaga tggcagccac gttgagcatt ggctcaatgg ggtaaaggtg atagattatg
    20041 atctgcagtc tgttgaaatg gattcgctaa tgaaaaatag caaatgggct aattatccgg
    20101 agttcggact tacgacctca ggccatattg ccttacagga tcacggtaac agggtttggt
    20161 ttcgaaatgt taaaatcaga tcgctttaat tcttaaccaa ctcttcaagg tcttctccgg
    20221 ccagcaaaat ggacgttttt gatgacttca tttccattat attgatggtt attctgtctt
    20281 tgtctgcagt attaatgaaa aaaagttttg atccgtctct tgaaaagacg gcatagaaag
    20341 attttattcc caaaatcggc tggttcttgc ttccatctag tgagatagtg tgaatttcat
    20401 ccgggtcggc tgcaaaaacg acagatttct catcacgcca tgatggataa taagaatgac
    20461 tatcgttatt tgtaatgtta cgtacttttt caccatttga attcattaca taaatttgat
    20521 ctttgccatc ccccttttca aaataataaa ctatatcatt tccgaaaggt gaccattttg
    20581 gattatagct tttatgctct ccactaaaag ttaactgtcg gtgattggtg ccgtccttgt
    20641 tacaaataaa aatttcaatc gttccggttt ggttactgtt ataaacaatt tgctgcccat
    20701 ccggtgacca tgaaccacca aaatttctat gctcattatc tgtgagctga aattcatttc
    20761 ctgaatctaa atcacgaaca aagatatttt gagattctcc ggtgcgtttt tcaaaaataa
    20821 tactattccc atcaggtgaa taagatgcat acaatccacc cgttgatatt tcaattaaac
    20881 ttttgtcagc aaggttatac tgaagaacaa cccagttttc cggtttaaaa ttagaccagt
    20941 ctgattttac actaattgcg gaaaagatta ttttatctcc tgcaggtgag cattccaggc
    21001 ttccaaagat aatccggttt tcatcctgac cgtaaatgag gttaactgct aaaaataacc
    21061 atccggttat gaatatcttt ttcatatgac agagcttata aaaagtatat acgcaaatct
    21121 aaagattaag atttgttctt taaatatacc ttatcaggta acaaaaggaa taaccgccct
    21181 tctttccacc ggatagtccg ggaattgttc cttataccaa cggtgatgat ttttagacct
    21241 tggaattaga ttcacaaaag tccatatcga aaatgaaaaa gccggcaaag accaggttaa
    21301 aatggcgaag ccaaaccact ccaggatttc gccaaaatga ttagggcatg aaacgtattc
    21361 aaacagaccg ccatagggaa ttttatatcc atccgaactg tttcttctta ttcggatcag
    21421 ttgattgtca gctgtccagt taatggcaag tccggcaata aagaaaaaca aacctataag
    21481 gaatctgcca tcataatacc agtgagattc atagaagggc gagagaaatc caaaatagta
    21541 tccattaaaa aatccattaa tgcaattgaa tataatcgca gaggcaacga tagcaaccgg
    21601 aatttttttt ccgctggttt tggttcttaa gggaaaaacc aatgctctgt ttacataatg
    21661 aaacacccac aggaacataa aaaaatccat gggcaggttt ttttcaccat taccgataat
    21721 gtataaagcg ggggcaacga tcagggcagg tagttccatt agaatccatc caatcttatt
    21781 atcaataagc ggcccccatt tgctggtggt atgtcggccg tagggttggg ttttccttct
    21841 cagaaacggg taggagagaa gggcaattag aatccagcct gtcaataggt aataatacgt
    21901 ttgcttttcc aaaaaaaaag aggtcattgg ttatgacctc ccgttaggtt t
//
LOCUS       DASWHX010000150         7669 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_883456, whole genome shotgun sequence.
ACCESSION   DASWHX010000150 DASWHX010000000
VERSION     DASWHX010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 7669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7669
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_883456"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..525)
                     /locus_tag="VGA21_10175"
     CDS             complement(<1..525)
                     /locus_tag="VGA21_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002701616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HEX9650917.1"
                     /translation="MIESTISFEEQIRRGIPVELPPPIPFDSSVNHAPKRKNILSRNE
                     KILSIKNALRYFPAKFHSELAIEFLAELETVGRIYMYRFMPHYDMVARPVEEYPCKSK
                     AAGAIMLMIQNNLDPKVAQHPHELITYGGNGAVFQNWAQYLLTMQYLSNMSDRQTLHM
                     YSGHPLGLFPSTVNA"
     gene            complement(529..1305)
                     /locus_tag="VGA21_10180"
     CDS             complement(529..1305)
                     /locus_tag="VGA21_10180"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX9650918.1"
                     /translation="MPDKKSISIALSGGGAKGICHLGVLQAFEEEKIQIDALSGTSSG
                     AMIAALYAYGYSPKEILNMVIKTSFFKLMKFTTTLNGLLTLDNLGKILIQHMPENDFN
                     VLKKPIYIAATELIKGKTVFFTEGELVKPILASCCVPVIFNPLKYKDGIYVDGGILKN
                     LPAEILRENNYDKVIGSDCNPIDHNFALTGVKKLIERSLLLAINGNNASSKEFCNVVI
                     EPPEMKKYSSFDLTKGKEIFEVGYIHTMDMVDEIKEKLNL"
     gene            complement(1306..2139)
                     /locus_tag="VGA21_10185"
     CDS             complement(1306..2139)
                     /locus_tag="VGA21_10185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015267126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX9650919.1"
                     /translation="MKLKVINAGGFKLDGGAMFGVVPKSLWNNINPADENNMCSWMMR
                     CLLIEENNSLILIDSGMGNKQSEKFFSYYYLYGEDTLLGSLKKAGVHPDEITDNFLTH
                     LHFDHVGGSVTKTSDGKLEPQFKNAVYWSNHDHWEWAVNPNDREKASFLKENIIPIQE
                     SGQLKFIEAGNELPFDDIKYVDGHTEKMMLPVLTYKGRKIVYMADLLPSVGHIPVAWV
                     MSYDVRPLQTLVEKEKFLNEAVENDYILFFEHDPKIECCTLKRTDKGIRVDQTFPLSD
                     I"
     gene            complement(2223..3170)
                     /locus_tag="VGA21_10190"
     CDS             complement(2223..3170)
                     /locus_tag="VGA21_10190"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009184701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HEX9650920.1"
                     /translation="MLLDFEKPIADLEAKLEDMKELATKSGSDVSSAVKSLEKRINEL
                     KKETFLNLTRWQRVQLSRHPDRPYTLDYIYEMTDTFIEMHGDRTVSDDKAMVGGFGEI
                     NGKSIMFIGQQKGRNTKQRQFRNFGMANPEGYRKALRLMKLAEKFNKPIVTLIDTPGA
                     FPGIEAEERGQGEAIARNLKEMMMLKVPVVCIIIGEGASGGALGIGIGDKVLMLENTW
                     YSVISPESCSSILFRSWEYKEQAAEALKPTAKDMLELKLIDGIIEEPLGGAHQDMKWM
                     SDLLKKNIVNSIKDLEKIDSDQRIQMRIEKFSKMGAFSE"
     gene            3251..3964
                     /locus_tag="VGA21_10195"
     CDS             3251..3964
                     /locus_tag="VGA21_10195"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9650921.1"
                     /translation="MPKVIIIGASTGIGRSLALELYSKNYQVAIASRKVEELKEISSK
                     CGNEVLYRQMDLQDLDESVATLSEMIFALNGIDILVLNSGIAAIYPSWKEEMKIIDVN
                     VKGFVALAHTGMDHFKQQGHGHLVGISSIASLVGFRKTTAYSGSKAFISNYMQGLRNL
                     SRKKKLNITITDIRPGFVLTPMTEQNLGMFWVATPEKAAKQIWQAIERKASVVYVTRR
                     WRLFAFLIRIMPGKLRDFF"
     gene            4049..4567
                     /locus_tag="VGA21_10200"
     CDS             4049..4567
                     /locus_tag="VGA21_10200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650922.1"
                     /translation="MINQLKELQLPKLVLVKNLTFPEPYREDPEFIDGKLIDSGHTIT
                     KLRDSIPSKLPEPFDEDKWLLYEYKFGSRNLADGYFTDLPMDLADELCDRKIFGESYT
                     IIWLVPPDMESILNVLRKLPLAGFGPEQIWEWYLDRMVVFPPKCLDAVKSQFELGFNF
                     TVQEKFTEIMNS"
     gene            complement(4571..4807)
                     /locus_tag="VGA21_10205"
     CDS             complement(4571..4807)
                     /locus_tag="VGA21_10205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650923.1"
                     /translation="MDRVKMIWEFSGEESEQFALHHAKHLEEFVIRKKIQNYSSGIES
                     VSASFSIAYLTVNKEEWEDVANVLRPHKAYVEKH"
     gene            4866..5456
                     /locus_tag="VGA21_10210"
     CDS             4866..5456
                     /locus_tag="VGA21_10210"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HEX9650924.1"
                     /translation="MRKILLMITMVFTTSVVFAQQPSKGAILIGGDLSFDIQKYTDKD
                     AVSGVSTETNQVGFGFGPRGGYVLFDGFLVGATLDFFTSRNKFGVDKFTSSTFQAGPF
                     VRYYLPFGLFGEVNFTGGVQSSEFTSGGTSSPRTTNSLTSGFLGAGFAYFLNSNVSLE
                     PLLGYGFDRTKLDNNDTITIGGLRFRVSLIVYLKNE"
     gene            complement(5493..7229)
                     /locus_tag="VGA21_10215"
     CDS             complement(5493..7229)
                     /locus_tag="VGA21_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009582344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9650925.1"
                     /translation="MSKSNVSIGHVFKTIIWPRRNLVFIGLILIIISRLASLILPGAS
                     KYLIDDVIANGDIPMLKILLLVVTGAILVQAITSFLLTKILSVEAQRLISILRAKVQK
                     QILQLPIRFFDNNKSGALVSRIMTDVEGVRNLVGTGLVQLIGGLLTSVISLILLINIS
                     PAMTLYVLVPVIIFGLVSLKAFAYIRPIFRERGVLNAEVSGRLTETLNGIRVIKGFNA
                     EDQETKTFEEGVEKLFLNVKKSLTSTSLVTSSATFLLGLASAGIMGIGGYLIIQNELT
                     FGEFLSFTLYLGFMIAPIIQMSNIGSQLTEAFAGLDRTEEIMNMDPEDDGLVRTIEIG
                     HIKGNIEFENVHFSYEEGKEVLRDISFQAAPGSVTALVGTSGSGKTTIAGLAASFLNP
                     LKGRILVDGIDLSAVKLSSYRNQLGVVLQDDFLFEGTIEENILFPRPKATSDQLINAV
                     KAAHVDEFTDRFEDGLKTVIGERGVKLSGGQRQRIAIARAILADPKVLILDEATSNLD
                     TESEMYIQESLKGLMRGRTTFVIAHRLSTIRQASRILVVEDGQIFERGTHNELIEKKG
                     RYFDLYTYQARI"
     gene            7381..>7669
                     /locus_tag="VGA21_10220"
     CDS             7381..>7669
                     /locus_tag="VGA21_10220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650926.1"
                     /translation="MAPLLASASFYVTGNPPDGHLMTLLLILVLLMIIYPIILYHAQP
                     KNLFHTILLHLVLYLLVSFPGMFIHRNPLSEIGGLIYFLMPSGITLALIISY"
BASE COUNT         2285 a         1585 c         1353 g         2446 t
ORIGIN      
        1 cgcattcact gtggaaggaa acaatcccaa tggatgacct gaatacatat gcaaggtttg
       61 ccggtcggac atgttcgaca gatattgcat tgtaagcaga tactgcgccc aattctgaaa
      121 tactgcgcca ttgcctccat aggtaatcaa ttcatgcgga tgttgagcca cttttggatc
      181 aagattgttc tgaatcatca gcatgatggc tcccgccgct ttagatttgc aagggtattc
      241 ttcaaccggc cgggccacca tatcatagtg gggcatgaat ctatacatat aaattctacc
      301 aaccgtttcc aactcagcta aaaattctat tgctaattca gaatgaaatt ttgcgggaaa
      361 gtatctcagg gcatttttga tcgacagtat tttttcattc ctggagagga tattctttct
      421 ttttggagca tggtttaccg aagaatcaaa aggtattgga ggcggcaatt ccacaggtat
      481 ccctctgcgg atttgctcct caaaactgat tgtggactca atcatgtttt acaaattaag
      541 tttttccttt atttcatcaa ccatgtccat agtatgaata taacccactt caaatatttc
      601 ttttcctttt gtaaggtcaa aagaagaata tttcttcatt tctggcggtt caataaccac
      661 attgcagaat tccttgctgc tggcgttgtt cccatttatc gcgagtaata aacttctttc
      721 aattaatttc ttcacacccg tcaatgcaaa gttatgatct attggattgc aatcagaccc
      781 aatgacttta tcataattat tttccctgag tatttcagcc ggcaggtttt tcaaaatacc
      841 accgtcaacg taaattccat ctttatattt aagtgggttg aaaattaccg gaacacaaca
      901 agaagccaga ataggcttaa ctaattctcc ttcggtgaaa aaaactgttt tccctttgat
      961 caattcagta gctgctatgt agattggctt tttaagcaca ttgaaatcat tctctggcat
     1021 gtgctgaatc aatattttgc caagattgtc aagagtaagt aacccattta aggtggttgt
     1081 aaatttcatc agcttaaaaa agcttgtttt aataaccata ttcagaattt cctttggaga
     1141 atatccgtaa gcatacaagg cagcgatcat tgcacctgaa ctggttccgg ataatgcatc
     1201 aatctgaatc ttttcctctt caaaagcctg caacacacca agatggcaaa ttcccttggc
     1261 accaccaccg gataaagcaa ttgatataga cttcttatct ggcatttaaa tatcacttaa
     1321 tggaaaggtc tgatctaccc tgattccttt atcggttctt tttaatgtgc agcattcaat
     1381 tttaggatca tgctcaaaaa aaagaatata atcattttcc acagcttcat taagaaattt
     1441 ttctttttca actaaagttt ggagcggtct tacatcataa ctcattaccc aggcaacagg
     1501 tatatgacct acagatggta aaaggtctgc catataaaca attttccttc ccttataagt
     1561 caagaccggc aacatcattt tctccgtatg accatcaaca tatttaatgt catcaaatgg
     1621 gagttcgttt ccagcttcta taaattttaa ttgtccactt tcctggatag gaataatgtt
     1681 ctctttaagg aaactggctt tctccctgtc atttggatta acagcccatt cccaatgatc
     1741 atggttagac caatagaccg catttttgaa ttgaggttct aacttaccat cgcttgtttt
     1801 cgttacagaa cctccaacgt ggtcaaagtg taaatgagtt agaaaattgt cggttatctc
     1861 atccggatga acgccggctt ttttaagact gcccagtaat gtatcttcac cgtataaata
     1921 ataataagaa aagaattttt cactctgttt gtttcccatt ccgctatcaa tcaaaatcag
     1981 actgttattt tcttcaatca acaaacaacg catcatccag gaacacatat tattttcatc
     2041 agcaggatta atattattcc aaagtgattt tggtacaaca ccaaacatcg cccctccgtc
     2101 aagtttaaat cctcctgcat ttataacctt caatttcatt ttgctttctt ttttgattaa
     2161 agatataaaa gaaaaagcca caaactctga atctgtggct tcaacttatt tagggctaat
     2221 aattactctg aaaatgcacc cattttagaa aacttttcaa ttctcatttg aattctttga
     2281 tcagaatcta ttttctccag gtcttttatt gaattgacaa tgtttttctt cagcaaatca
     2341 gacatccatt tcatatcctg gtgagcgcca cccaatggtt cctcaattat gccatcaatt
     2401 aatttcagtt cgagcatatc tttggcagta ggtttaagcg cttcagcagc ttgttctttg
     2461 tattcccagc tacggaataa aatcgatgaa caggactctg gtgagattac agagtaccag
     2521 gtattctcaa gcatcagaac tttatctcct attcctatac ctaaagctcc gccggaagca
     2581 ccctcaccaa taataatgca gacaaccggc accttcaaca tcatcatctc ttttaagttc
     2641 cttgcaatgg cttccccctg tcctctttct tcggcttcta ttccggggaa tgctcctgga
     2701 gtatctatca acgtaactat aggtttgtta aatttctcag caagtttcat caggcgcagt
     2761 gcttttcgat agccttcagg gttcgccatt ccaaaatttc gaaattgtcg ttgtttagta
     2821 tttctaccct tttgctgacc tatgaacatt atacttttcc cattgatctc tccaaaacct
     2881 cccaccattg ctttatcgtc agatacggtc cggtctccat gcatttcaat gaaagtatcc
     2941 gtcatctcat aaatataatc taaagtataa ggcctgtcag gatgccggct taactgaaca
     3001 cgctgccagc gggtgagatt taggaaagtc tctttcttta gttcattaat tcttttttct
     3061 aaagacttaa ctgcagaaga aacatctgag ccgcttttgg tagccaactc tttcatatct
     3121 tctaactttg cttcaagatc tgctataggt ttttcaaaat ccaagagcat agggtctaaa
     3181 tttgaggtac aaaattagaa tataatctta tattgaagcc taattattaa agttttatcc
     3241 ctaatccagg atgccaaaag taattattat cggtgccagc acgggaattg ggagatccct
     3301 tgccctggaa ttgtattcaa aaaattatca ggttgccatt gcatcccgca aagttgaaga
     3361 attaaaggaa atctcatcaa aatgtggtaa tgaagtattg taccgtcaaa tggatcttca
     3421 agatcttgat gaatcggttg cgacgttgag tgaaatgatt tttgctctta acggaataga
     3481 tatactggta ctgaattcag gcatcgctgc catttatcca tcgtggaagg aagaaatgaa
     3541 aattattgat gtaaatgtaa aagggtttgt agctctggct cataccggca tggatcattt
     3601 taaacaacaa gggcatggac atctggttgg aatctcttca atcgcttcat tggttggctt
     3661 cagaaagact acggcatact caggaagtaa agcttttatt tccaattata tgcaaggact
     3721 tcgaaatttg agtagaaaga aaaagttaaa tataacaata actgatattc gacctggctt
     3781 tgtattgaca cccatgacgg aacaaaacct tggaatgttt tgggtggcta ctccggaaaa
     3841 ggcagcaaaa caaatttggc aagcaattga acgaaaagct tcagttgttt atgtgacaag
     3901 acgatggaga ctttttgcat ttcttatccg gattatgcca ggcaagctca gagatttttt
     3961 ctagactgat tttgatagtg taacaaaaaa aatcaatttt caatttcata gtctttttca
     4021 tataattgct aacagatttg aggaattcat gatcaatcag ttaaaggagt tacaattacc
     4081 caagttggta ctggtaaaaa atctgacgtt tcctgagccc tacagagaag accccgaatt
     4141 tattgatggc aaactaattg acagcggtca cacaattaca aaactgcgag atagcattcc
     4201 gagcaaatta cctgaacctt tcgatgaaga taaatggtta ctttatgaat acaagtttgg
     4261 cagcaggaat ctggcagatg ggtactttac agatctgccc atggatttag cggatgaact
     4321 ttgtgacaga aaaatcttcg gagaaagcta cacaattata tggttagtcc cgcccgatat
     4381 ggaatccatt ctgaatgtac tcaggaaact gccactggca ggttttggtc ctgaacaaat
     4441 atgggaatgg tacctggaca ggatggtagt atttccacct aagtgtcttg atgcggttaa
     4501 aagccaattt gaattaggat ttaattttac ggttcaggaa aaatttacgg aaataatgaa
     4561 cagttaaatt ttaatgcttt tccacatatg ctttatgggg ccttagcacg ttggcaacat
     4621 cctcccactc ctctttattt acagtcaggt atgcaattga aaaactggcg gaaactgact
     4681 caatgcctga cgaataattt tggattttct tcctgatcac aaattcttcc aaatgctttg
     4741 catgatgcaa agcaaattgt tcagactcct caccactgaa ttcccaaatc atttttactc
     4801 tatccacgta taataattag ataaacaaat ttgtatctta caataactta acaaacatta
     4861 cgaagatgag aaaaatatta ttaatgatta ccatggtgtt tacaactagt gttgtctttg
     4921 cacagcaacc ttctaagggt gcaattctga tagggggtga tttatcattt gatatccaaa
     4981 aatatacgga taaggatgcc gtctctggag tatcaactga aacaaaccaa gtgggttttg
     5041 gatttggacc gagaggcggc tatgtgcttt ttgacggatt cctggtcggg gcaacattag
     5101 atttttttac atcaagaaat aaatttggtg tggataaatt taccagttcc actttccagg
     5161 ctgggccatt tgtgagatat tacctgccct tcggattatt tggggaagta aattttacag
     5221 gcggtgtgca atcaagtgag ttcacatcgg gaggtacgtc aagtccgcga actacgaata
     5281 gtttaacttc cggttttctg ggtgccggat ttgcttattt tctaaattca aatgtaagtt
     5341 tggagccatt attgggttat ggtttcgaca ggactaaatt ggacaacaat gatacaatta
     5401 caattggtgg attgcgtttc cgcgtatcac ttattgtata tttgaagaat gaataattag
     5461 gaacaattta gtagcctggc actgacaggc tactaaattc ttgcctggta agtatacagg
     5521 tcaaagtacc ttcccttctt ttcaataagt tcattatgag taccccgctc aaagatctgg
     5581 ccgtcttcaa ccactaaaat gcggcttgcc tgccttatgg tactaagcct gtgcgcaatt
     5641 acaaaagtag ttctacctct catcaatcct ttaagacttt cctggatata catctcgctt
     5701 tcggtatcga gatttgaggt ggcttcatcc agtattaata ccttcggatc agcgagaatt
     5761 gcccgggcaa tggcaattct ttgccgttgc cctcctgaaa gctttactcc gcgctcacca
     5821 attactgttt ttaatccatc ttcaaacctg tcagtaaatt catcaacatg agctgcttta
     5881 accgcattga tcagttgatc agatgtagct tttgggcgtg gaaataaaat attttcttca
     5941 atagtacctt caaaaaggaa atcatcctgg agcaccactc ccaattggtt tcgataactt
     6001 gataacttca ctgcagacag atcgatacca tccaccaaaa ttcttccctt cagcggattt
     6061 agaaatgatg cagccaggcc ggctatcgta gttttaccag atccgctcgt tccaacaagt
     6121 gcagtaactg aacccggcgc agcttgaaat gaaatatctc tcagtacttc tttaccttct
     6181 tcataggaaa aatgaacatt ctcaaattca atattacctt ttatatgacc aatttcgatg
     6241 gtccttacca gcccgtcatc ttccggatcc atgttcataa tttcttcagt acgatccaaa
     6301 ccggcaaaag cttcggtaag ctgactgcca atattactca tttgaataat tggcgcaatc
     6361 ataaatccca ggtataaagt gaaactcaaa aattcaccga aagtgagttc attctgaatg
     6421 attaaatacc caccaattcc catgattccc gctgaagcta atcctaatag aaaagttgcc
     6481 gaacttgtaa caagacttgt tgaggtaagg ctcttcttaa catttaagaa taatttctct
     6541 actccttctt caaaggtttt ggtttcctgg tcttccgcat taaatccttt gattaccctg
     6601 attccgttca aagtttctgt taaccttccg ctaacttcag cgtttagaac tccccgttcc
     6661 ctgaaaatgg gacggatata ggcaaaagcc ttcagcgaca ctaatccaaa aattatcacc
     6721 ggcaccaaca cgtataaagt cattgccgga ctgatattta tcaggagaat taacgaaata
     6781 acagatgtaa gaagacctcc aataagttgg acaagcccgg ttccaacaag attacgaacg
     6841 ccttcaacat ctgtcataat cctggaaaca agcgcaccag atttattatt atcaaaaaag
     6901 cgaattggta attgaagaat ctgcttttga acctttgctc ttaaaattga aatcaatctt
     6961 tgggcttcaa cactgagtat cttagtgagt agaaagctgg ttattgcctg aaccagtata
     7021 gcgcctgtaa ccaccagcag taatatttta agcataggaa tatctccatt tgcaatcaca
     7081 tcatcaatca ggtatttact ggcaccaggc agaatcagac ttgccaacct gctgataatg
     7141 attaaaatca agcctataaa aacaagattt ctgcgcggcc atataatggt tttgaataca
     7201 tgaccaatac taacatttga tttactcatc cacggatttt tacaaggttg acgattaata
     7261 ggacatttta ctttaaatta acggaaaatc cttcgcaaga tacttcattg tgtattaaac
     7321 catttgcctt tttccgttta gaagataaga ttgaaaacat cgagattaat tttgtatctg
     7381 ttggcaccat tactagcctc ggcttcattt tatgtcaccg gcaatcctcc cgatggacat
     7441 ctgatgacgt tacttttaat tctggttttg ctaatgataa tttatcccat tattctttat
     7501 catgcacagc cgaagaactt attccataca atccttttgc atttggtgtt atacttactt
     7561 gtttcatttc ccggtatgtt cattcaccga aacccacttt cggaaattgg cggtctgatt
     7621 tattttttaa tgccttcagg gattactttg gcattgatta tttcatatc
//
LOCUS       DASWHX010000151        13167 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_884080, whole genome shotgun sequence.
ACCESSION   DASWHX010000151 DASWHX010000000
VERSION     DASWHX010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 13167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13167
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_884080"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2083
                     /locus_tag="VGA21_10225"
     CDS             <1..2083
                     /locus_tag="VGA21_10225"
                     /inference="COORDINATES: protein motif:HMM:NF013125.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HEX9650927.1"
                     /translation="LTPEIETDSIIYLNDNPSFRIYLADSIPNESPYHVYISGVADCS
                     GNILDDEFTLQLDFLPPAILDVQIHHYNELELSFNEPPVASSISKNSFYLEDIVPAKV
                     TVIDSVHAVLSFDQELIDFQSYTLSFQNLKDIEGNAGGGSFNFIYTAPPEPGFLELII
                     TEIMVDPEPGLGTLPVSQYVEIFNTSSKKFSLKGLQLGDLNSTAISNAGILDPLSYIL
                     LVPSTQKINFPDGNKIIGLTNWPSLNNTGEIMSIKNRKGGLLHQIEYSNKWYKELEKQ
                     EGGWSLEMIDTNNPCGEAANWGASISDIGGTPGLVNSLLAENPDNFGPMLTSVIPIDS
                     VTLFLEFNEPIHANKLPGLSISPEIAIQSVNFKENSFTKILVKLEAPLSEKVSYHIII
                     QAADCKGNVSSIESDEFYLPEESVYNDIVISEILFNPFTGGIDFVEFFNRSDKHLLLS
                     DWTSYSYTQSGKTGSEIITSDYFILKPKAYLIISEDKLIFSNLYPFVDLNKVIEMNLP
                     TLPDEEGTLVLRNTHNIVVDSVYYNEDWHHPLLKSKEGVSLERRSPDISSVDPNNWKS
                     ASSTVSFSTPASENSQSYNPVPDDSEVDVFPKLFFPGTGGTDSFATIEYSLGNPNLLA
                     SINIFAGNGLLVKRIAVNQLLATEGIFTWDGADDSGNKVTLGHYFVVFDVFSPDGYKK
                     RYRKKVAVGVR"
     gene            2190..2327
                     /locus_tag="VGA21_10230"
     CDS             2190..2327
                     /locus_tag="VGA21_10230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650928.1"
                     /translation="MVCQGTDHGGGSGGGSQVINGLDGGRPWKGNGLHGGRPRRGRWG
                     N"
     gene            2530..3681
                     /locus_tag="VGA21_10235"
     CDS             2530..3681
                     /locus_tag="VGA21_10235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015264576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HEX9650929.1"
                     /translation="MSKYDRRGVSFGKEEVHQAIKNLDKGIFPNAFCKVNEDILGGDP
                     DFCNIVHADGAGTKSSLAYLYWKETGDLSVWKGIAQDAIVMNTDDLLCAGVAGNILIS
                     STIGRNKKLIPGEIIKTIIEASEEFYALMHDYGFDITSTGGETADVGDLVRTIIVDSS
                     VTARLPRKDILEVSPKPGDAIIGMASFGQAIYESEYNSGIGSNGLTSARHDVFHNSYA
                     KKYPETFDSAIQKDLVYSGKYKVTDTVPGININIGKLVLSPTRTYAPVLVEILKEFRS
                     NINAIVHCSGGGQTKILHFIENLHIIKDNLFETPALFKIIQEESNSDIKEMYRVFNMG
                     HRMEIYLPRTYAEGIIAIANSFNVDAKIVGRVEEAKGKFLTIGDLKLTG"
     gene            complement(3806..4948)
                     /locus_tag="VGA21_10240"
     CDS             complement(3806..4948)
                     /locus_tag="VGA21_10240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002693662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX9650930.1"
                     /translation="MKSHLSKSTTCVQSGGIQDSVTGGVVTPIYPSTAIDYIHVDETM
                     YPRYFNTPNQKVVAEKIAALENGERAIIFSTGMAAIMTAAFTFLRKGDHAIFQHDLYG
                     GTQNAIKTEFKKFGIDYTLVDATEIDNIENAILPNTRLIFIETPSNPILKIVDISKVA
                     KLGKKNKIITMIDNTFATPINQTPLDLGMDIVMHSATKYLGGHSDLISGVLVTSNKLA
                     KQIGKSAINYGGSVNAITCYLLERSLKTLAIRVEIQNKNAMVLAKYLENHFRIERVYY
                     PGLKNHHNHTIARKQMSGFGGMVSFEIKGGVKASSRFISKLKIIKSAVSLGGIETILC
                     APAKTSHAKISSEERMKLGIKDSLIRMSVGIEGIEDLKQDIKNALR"
     gene            complement(4963..5547)
                     /locus_tag="VGA21_10245"
     CDS             complement(4963..5547)
                     /locus_tag="VGA21_10245"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX9650931.1"
                     /translation="MNVTDLNKLYGSLDIYLLDAIMKGYFEEKNSVLDVGCGEGRNLV
                     YFLQSDYEVHGIDKNESCIRLCNMMAKSYRTNFDKLNFQCSDATTLPYADDYFDIVLS
                     SAVMHFARDEAEFLSMMSELIRVLKRHGIAFIRMASTIGSDKIILENGFSFLLTRDLI
                     EHCLKKFHLEKLEPVKSVNVEDKRVMTTLLLKKK"
     gene            5699..7291
                     /locus_tag="VGA21_10250"
     CDS             5699..7291
                     /locus_tag="VGA21_10250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650932.1"
                     /translation="MKLRYGLVKLILLFLLCVFVTSCFNEFSDLDNIKKFKWEPTIAV
                     PIIDSEFSFKDFVTEISDSSVVYSRDDGLIVFAYRDTVISKTGESLITIPNQDFQYSN
                     DFPAPLIIQFPFNDSIIYAEEIKFDFSTQSGQELDSILLKDGILNFGINWNFNQPGKL
                     EIEFKSLLQNGQPITINHEFASSLNYSESFPLQDIKVDLTDQGTSTNSFLIDLSFTIY
                     NNGSPLLLNPSFDINLIVTDPKFKAVFGELGSIPFNTDIGTTKLKFFENMEFGIIELE
                     DPTAILTISNSFGMGVTTYIDDISIAHTTGTTKLEGSITTQPISVNAVPLANMGNFEI
                     SVIEINNGNSNLKELIKYVPQEISIAFNGAVSNPGSKDFVLDNSTVYLDLMVELPLYG
                     KVSELGISHDFDFDGSIFDDLDNALLQFKSFNEFPIDMTIQATFLSQTGIPLLEFFTL
                     DNNLLVASENDLNGMVVNRGENVLVVELDRDDLETASRSEFIRLDVEVSTFNNGSVPV
                     KILDSHRITFKVGLQSKFVNND"
     gene            7284..8612
                     /locus_tag="VGA21_10255"
     CDS             7284..8612
                     /locus_tag="VGA21_10255"
                     /inference="COORDINATES: protein motif:HMM:NF040164.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5723 family protein"
                     /protein_id="HEX9650933.1"
                     /translation="MIRLNVVVLFLILPLTVIAQGYMGFYSLEGRVPQTNLINPSFFP
                     EAKVVVAFPALGSTSVFLDADRFSFSRILVKDGDSLRFEPDNFVNKLQKKNRLEVGVD
                     MNLFYLGVQARKNYFSISANTKVTSNLLISKKLIEWALYGPADERNINQPLDFSDLGG
                     SVMAYNEFTLGYGRKLLGDRLQVGIRFKYLNGIAEASAKNINGYIYAGIDSIQLYSEA
                     FTINTSGIDDFDNNPEDLIFGFKNPGFAIDIGAQYQINSKFSVSAAFTDMGSIKWKNS
                     LKGWEFNEVNYVFRGFDVLDVINESDVIDNELDSLENLYDINEIEGKAYRAPLVTKTY
                     LGGRFQFHPKQNVSLILYNDFFQGNVKSALGLNYGLLVGRSMDLLVGLSYRDRSLANF
                     TFGTAFSLGFFQLYFLSENFPSLFMPQNARKLDLRFGINFQFGRKRKGSS"
     gene            complement(8613..12194)
                     /locus_tag="VGA21_10260"
     CDS             complement(8613..12194)
                     /locus_tag="VGA21_10260"
                     /inference="COORDINATES: protein motif:HMM:NF020083.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HEX9650934.1"
                     /translation="MANEVWEEIYDRVEELITAHNTTLIFVNTRRLAERMSHNLSNRL
                     GEGTVTAHHGSMSKEHRLDAENRLKGGKLKAVIATASLELGIDVGGIDLVCQIGSPKS
                     IAKFLQRVGRSGHTVNGTPKGIIFPLSRDEMIECAALLHSIKLGELDEIIIPVKPLDI
                     LAQQIVAELSSRDYEEDELFHLIRKSYPYKEVTRQEFDEIIQMLSEGYTTRRGRRGAY
                     VHHDQINGKLKGRRGSRLTALINSGAIPDNFDYDVIMEPRNLFIGTLNEDFAVESMPG
                     DVFQLGNNSYRILKVETGKVRVEDAMGAPPSIPFWFGEAPGRTRELSEAVSRLRTRIG
                     ELVGSPDWEKIGEDDDNSWKTQAMEWIKAEISLPEEICDQIVTYLAATQAALGVMPSR
                     DNLVMERFFDEAGDMHLVIHSPFGSRMNRAWGLALRKRFCRKFNFELQAAATEDNIIL
                     SLGATHSFAIDEVFSYLNPKTVRDVLIQALLDAPMFEVRWRWNATRALAILRRRGGKK
                     VPPQIQRSQAEDLVALVFPDQLACLENIVGEREIPDHPLITQTIEDCLYEAMDIIELE
                     KLLTEIKSGKKTLVGKDLRGPSPMAQEIINARPYSFLDNAPLEERRTNAVQSRGWLDP
                     KQAGELSKLDPEAIRAVKEEAWPQVLNEDELHDSLILFSFFSEEEILANTWNEFLEVL
                     HSDNRVVDIKLNSGRKLWVAAERIPQFARIFTVELSKIEINLPEKIASQKWDEEDALR
                     EIVRGRIEAMGPVESMDLVQLMELPITKIEQALMALENEGFVLRGSFHGNDEITEWCD
                     RRLLARIHRYTLQTLRKSIEPVSSSDFMRFLFVWQGVDVENKKEGPDGLMKVLEQMEG
                     YEAPAAAWEGDLLPVRVKDYDFFWLDMLCLSGRILWGRFRVNTDNGSKMSSPIKASPI
                     MLIQRDNIGIWKNKLTDVAGLSSNGKLVRDILNENGASFFKDIVNSSGLLALQAEEAI
                     AELIASGLVTSDSYTGLRALLTPAKFKSRRSKNVFSMEQAGRWSIINNESESINEEER
                     TKQLSKVYLRRYGVVFRKLIDKEQGSPPWRDLLKVYRRMEARGEIRGGRFVDGVWGEQ
                     YALPEAVSLMRKTRNDKEKAKLVAISAGDPLNLTGIITTGHRVASITSNRILYEDGEP
                     VVMKEGKEVKFLKQFESPKKWRYKNALITREIPPKLRSYLGRGQIAE"
     gene            complement(12194..12952)
                     /locus_tag="VGA21_10265"
     CDS             complement(12194..12952)
                     /locus_tag="VGA21_10265"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX9650935.1"
                     /translation="MKPDHQNLQSLFNPIVWKWFKENVGEPSEVQTKAWPVIKSSNNV
                     LISAPTGSGKTLSAFFSILDNLFVQSLDGNLEDETQVIYVSPLKALSNDIEKNLQTPL
                     NGISRYYKREGKEVEVRVGLRTGDTTPAQRTAMIKRPPHIIVSTPESLYLLLTSENGR
                     KMLSTTRTIIVDEIHAMLGDKRGSHLSLSIERLEKLADKSLVRIGLSATQKPIEKVAD
                     FLVGNNTRKCSIINAGHKRPLELKMEYPDPRWRL"
     gene            complement(12958..>13167)
                     /locus_tag="VGA21_10270"
     CDS             complement(12958..>13167)
                     /locus_tag="VGA21_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010609784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9650936.1"
                     /translation="FTVAPGFTRTEMAQVFIDKYGNDYATGDIALSELTEPKDIAPTV
                     LFLASGLANHATGATIDINAGSYVH"
BASE COUNT         3714 a         2802 c         2582 g         4069 t
ORIGIN      
        1 tttaacgccg gaaattgaaa ctgatagtat aatatatctg aatgataatc cttcctttag
       61 gatttacctc gcggacagta tccccaatga atcaccgtat cacgtctaca tttcaggggt
      121 tgcggattgt tccggcaata tccttgatga tgaattcact ttgcaattgg attttctgcc
      181 accagcaatc ctggatgttc agatccatca ttacaatgaa ttggaactgt cctttaatga
      241 gcctccggta gcatcttcca tttcaaaaaa tagtttttat ctggaagaca ttgtcccggc
      301 aaaagttact gtcatcgatt cagtccatgc tgttttaagt tttgaccagg aattaattga
      361 ctttcaaagt tacacgctgt cttttcagaa tctgaaagac atcgaaggaa atgcaggcgg
      421 cggaagtttt aatttcattt acaccgcgcc tccggaaccc ggatttctgg agctgataat
      481 tactgaaata atggtggacc ctgaacctgg cttaggcaca ttgccagttt cacaatatgt
      541 agaaattttt aatacttcat ctaaaaagtt tagtttgaaa gggttgcaat taggagactt
      601 aaattccact gctatcagta atgcaggaat attggaccct ctttcctata ttttattagt
      661 gccatcaacc caaaaaataa actttccaga tggtaacaaa attattggac taacgaattg
      721 gccatcactg aataataccg gtgagatcat gtctatcaaa aacagaaagg gaggcttact
      781 tcatcagatt gaatactcaa ataagtggta taaggaactg gaaaagcaag aaggcggctg
      841 gtcattggaa atgatcgaca cgaataatcc ttgcggagaa gctgccaact ggggagcatc
      901 aatctctgat attggcggaa caccaggtct tgtcaactct ttgcttgctg aaaatccgga
      961 caattttggt ccaatgctaa cctcggttat tcctattgat tcggtaaccc tgtttctgga
     1021 attcaatgag ccaattcatg ctaataaact tcccggactt tccatctccc ctgaaatagc
     1081 tattcaaagt gtgaacttta aggaaaacag ttttactaaa attctggtta agctggaggc
     1141 tcccctgagt gaaaaagttt cctatcatat tattattcag gctgcggatt gcaaaggtaa
     1201 tgtaagcagc atagagtctg acgaattcta cctgcccgaa gaaagtgtat ataatgacat
     1261 cgtgatcagt gaaattcttt ttaatccgtt taccggtggg atagactttg ttgaattctt
     1321 caaccgatcg gacaagcatt tgctcctctc cgactggacg agttacagct acacccaatc
     1381 agggaaaaca ggatcagaaa ttataacgtc tgattatttt attctgaaac caaaggcgta
     1441 tctgattata tcagaggaca aattaatttt ttcaaatctt tatccatttg ttgatctgaa
     1501 taaagtcatt gaaatgaatc tgccaacttt accggatgag gagggaacct tggtcttgag
     1561 aaatacacac aatattgtgg tggattcagt gtactataat gaagactggc accatcctct
     1621 gcttaagtca aaggagggtg tctctctgga aagaagaagt ccggatattt catcggttga
     1681 ccccaataac tggaagtccg catcatctac ggtttccttt tcaacaccgg ccagtgagaa
     1741 ttctcaatct tataatccgg ttcctgatga ttctgaagtt gatgtatttc cgaaattatt
     1801 tttccccgga acagggggca ccgattcgtt tgccaccatt gaatattccc ttgggaatcc
     1861 taatcttctg gcttccataa acatctttgc cggcaatggg ttactggtaa aaagaatagc
     1921 tgtcaaccag ctgttggcca ccgagggtat ttttacttgg gatggagcgg atgattcagg
     1981 aaacaaggtt actctcggac attattttgt ggtgtttgat gttttctcac cggatggata
     2041 caagaaacgg taccggaaga aagtggcggt gggagtcagg taataatggt ctgtcaggga
     2101 gattaatagt ctgtcagggg acagaccatg gaaaggggaa gaaagtggcg gtgggagtca
     2161 ggtaataatg gtctgtcagg gggagattaa tggtctgtca ggggacagac catggaggcg
     2221 gaagtggcgg tgggagtcag gtaattaatg gtctggatgg aggcagacca tggaagggga
     2281 atggtctgca tggaggcaga ccaaggaggg ggaggtgggg taattaatgg tcgagaggag
     2341 gtaatattaa aaatggtctg tcaggggaca gaccatggaa aattaaaaga gaaaaaaatg
     2401 aaattggtga atcaaaatat attaacaatt gctcaaaagt ggtcaactct aactaggaca
     2461 cctcaaattc ctaattccta attcctaatt cctaattgaa cttttgctca ttgttcctac
     2521 ctttgcgcta tgtctaagta tgaccggcgc ggagtctcat ttggaaaaga ggaggttcat
     2581 caggcaatta aaaacctgga caaaggaatc tttccaaatg cattttgcaa agtgaatgaa
     2641 gatattctgg ggggtgatcc tgacttttgt aacattgtgc atgccgacgg agccggaaca
     2701 aaatcttccc tggcatacct ctattggaaa gaaaccggcg acctgtcagt ctggaaagga
     2761 attgcccagg atgctatcgt gatgaatacc gatgatcttt tatgtgcggg agtagccgga
     2821 aacattttga tctcttctac catcggcaga aataagaagt tgattcccgg tgagattatc
     2881 aaaaccatca ttgaagcctc tgaagaattt tacgcattga tgcatgatta tgggttcgac
     2941 atcacaagta ccggaggcga aacagccgat gttggtgacc ttgtacgaac cattattgtt
     3001 gacagctccg ttactgcccg tcttccgcga aaagatatcc ttgaagtgag tccgaagccg
     3061 ggagatgcaa ttatcggaat ggcctctttt ggccaggcga tttatgaatc agagtataac
     3121 agcgggatag gcagcaacgg tcttacctca gcccgccacg atgtgttcca taattcttat
     3181 gcaaaaaaat acccggagac attcgattct gccattcaga aagatttggt ttattcgggg
     3241 aaatacaagg taacggatac tgttccgggg ataaatatta atattggaaa actggtgctt
     3301 tccccaacaa gaacttacgc ccctgttctg gtagaaatac tcaaagagtt caggtcaaat
     3361 attaacgcga ttgtgcactg cagtggcggc ggacaaacca agattttgca tttcatagaa
     3421 aaccttcata taatcaagga caatttgttc gaaacacctg ccctcttcaa aataatccag
     3481 gaggaaagca attccgatat aaaagaaatg taccgcgttt tcaatatggg gcatcgcatg
     3541 gaaatctatc ttccccggac atacgccgaa ggtataattg caatagccaa cagctttaac
     3601 gttgatgcaa aaattgtggg aagagtagaa gaagcaaaag gcaaattcct aactattggt
     3661 gatctgaaac tgacaggtta attttccaaa tcatcatacc atctttaact tttcaagtca
     3721 gctcttgtga attgtatctt tgcccaatta aattaaaacc tgctgaattc atgaattgtt
     3781 tgaataaacc tacatgacct ccaggctatc tcaaagcatt tttgatatcc tgcttcaaat
     3841 cttcaattcc ttctattccc accgacattc gaataaggct atccttgatt cccaatttca
     3901 ttctttcttc ggaagaaatc ttcgcatgag aggttttagc cggagcgcac aagattgttt
     3961 caattccgcc aaggctaact gcagacttaa taattttcag ctttgaaatg aatctcgatg
     4021 aagccttcac acctcctttt atttcaaatg aaaccatgcc gccaaagccg ctcatttgct
     4081 ttctggcaat ggtatgattg tgatgatttt ttaaccccgg ataataaact ctttcaatcc
     4141 tgaagtggtt ctcaagatat ttcgccaata ccattgcatt tttgttttga atttctaccc
     4201 ttatcgccaa agtttttaaa cttcgttcca ggagataaca tgtaatcgca tttacactcc
     4261 caccgtaatt tattgctgac ttcccgattt gtttggctaa tttattactc gttacaagga
     4321 caccgctaat caaatcacta tgcccgccga gatatttagt agcactgtgc atcactatat
     4381 ccattcccag atctaacgga gtttgattaa ttggagtggc aaaggtatta tcaatcattg
     4441 ttatgatttt attctttttc cccagtttag ccactttaga tatatcaaca attttcaaaa
     4501 ttggatttga aggggtctcg ataaaaataa gtcttgtgtt tggaagaatc gcgttctcaa
     4561 tgttgtcgat ttcggttgca tccacaagtg tgtaatcgat cccgaatttc ttaaactctg
     4621 ttttaatcgc gttttgcgtt cctccgtaaa gatcgtgttg aaaaattgca tggtctcctt
     4681 ttctaagaaa agtaaatgcg gcggtcataa tggcagccat tcccgtactg aatattatcg
     4741 ctctttcacc attttccagt gccgcgatct tttcagcaac cactttttgg ttcggtgtat
     4801 taaaatacct gggatacatt gtctcatcta cgtggatgta gtcaatagct gttgagggat
     4861 aaatgggggt tactactcct cctgtgaccg aatcctgaat tccaccactc tgaacgcagg
     4921 ttgttgattt ggataaatgt gatttcattt tcatgctgta atttatttct tttttaaaag
     4981 tagcgtggtc atgactcttt tgtcttccac attcactgat ttcacgggtt caagtttttc
     5041 cagatgaaat tttttgaggc aatgctcaat caggtcacgg gtcaataaaa aactgaatcc
     5101 attttctaaa attattttat cacttccaat tgtggaagcc attctgataa aggcaattcc
     5161 atgtctcttt aacaccctga ttaattcact catcatggaa aggaattcag cttcatcccg
     5221 cgcaaaatgc attaccgcac tacttaaaac aatgtcaaaa taatcatcgg cgtatggtaa
     5281 tgtggtagca tctgaacact gaaaattgag cttatcgaaa ttagttcggt atgatttggc
     5341 catcatattg caaagcctta tgcaagactc atttttgtca attccatgga cctcgtaatc
     5401 actctgtaaa aaatacacca gattacggcc ctcgccgcaa cctacgtcaa gcaccgaatt
     5461 tttctcttca aaataacctt tcataattgc atccagcagg taaatgtcca aacttccata
     5521 caacttattt agatcagtta cattcatgct ttttatcata tatatgaaag ccaaatgtcc
     5581 gtatatttgg cctcttttaa aaaaatggta tgaaaaactc actttattta ggccacaagg
     5641 taaagcttat agactcatgc aaggtcggaa gaacagcaga aacttcttta atatcattat
     5701 gaaattgaga tacggattag tgaaattgat tttactattt ttgctgtgtg tctttgtaac
     5761 aagttgcttt aatgaatttt ctgatcttga taacataaag aaattcaaat gggaacctac
     5821 tattgctgtc cccattatag attctgaatt ttcatttaaa gatttcgtta ccgaaatatc
     5881 tgattccagt gtagtatact ccagggatga cggactcatt gtatttgctt accgggacac
     5941 cgtgatttcc aaaactggcg aatcgctaat cacaatccct aaccaggatt ttcaatattc
     6001 caatgatttc ccggcaccat tgattattca atttcctttt aatgattcca tcatatatgc
     6061 ggaagaaatt aagtttgatt tctcaaccca atccgggcag gagttagatt ctatcttact
     6121 taaagatggc atattgaatt ttggaattaa ttggaacttt aatcaaccag gcaagcttga
     6181 gatagaattc aagtcacttc tccaaaatgg acaacccata actattaacc atgaatttgc
     6241 ttcatcactt aattattccg aatcatttcc ccttcaagat attaaagttg atttaaccga
     6301 tcagggaacc agcaccaata gttttttaat cgatctaagt tttactatct ataataatgg
     6361 cagtccctta ctactaaatc cgtcatttga tattaacctg attgtaactg atccaaagtt
     6421 taaagctgtt tttggagagt tgggttccat tcctttcaat accgatatag gaacgaccaa
     6481 gcttaagttt tttgaaaata tggagtttgg aattattgaa cttgaggacc ccacagctat
     6541 cctcaccatt tcgaattctt ttggcatggg tgtgactacc tatattgatg atattagcat
     6601 tgcccataca actggtacca caaagcttga aggaagtatt actactcaac caatttctgt
     6661 gaatgcggtt ccgcttgcca atatgggaaa ttttgaaata tcggtgatag aaattaataa
     6721 tgggaattca aatttaaagg agttgatcaa atatgttcca caagaaattt ccattgcatt
     6781 caatggtgca gtcagtaatc ccggttcaaa ggattttgtg ctggataata gcaccgttta
     6841 ccttgacttg atggtagaac ttccattata tggtaaggta agcgagctcg ggatatctca
     6901 tgattttgat tttgatggga gcatatttga tgacctggac aatgctttgt tgcagtttaa
     6961 atctttcaat gaattcccga ttgatatgac aatacaggca acgttcctca gtcaaaccgg
     7021 aattcctctg cttgaatttt ttacccttga caataacctg ctcgttgcat ccgaaaatga
     7081 tctgaatgga atggtggtga accgtggtga gaatgttctg gttgttgaat tggatcgcga
     7141 tgatcttgaa actgcttcaa gaagtgaatt cattcgtttg gatgtggaag tttcaacttt
     7201 taataatggt tccgttcccg taaaaatact cgattcccac cggattactt ttaaagtagg
     7261 cttgcaatca aagtttgtga ataatgatta gactaaatgt tgtagtactg tttctaattt
     7321 taccgctaac cgttattgct cagggttata tgggattcta tagcctggaa gggcgtgtac
     7381 cgcaaactaa cttaatcaac ccttcatttt tccctgaagc caaagtggta gttgcatttc
     7441 cggcattggg ttcaaccagt gtattcttgg atgctgatcg ttttagcttt agcagaattc
     7501 ttgtaaaaga tggcgattca ttacggtttg aaccggataa tttcgttaat aagctgcaga
     7561 agaaaaacag gttggaagtg ggcgttgata tgaatttgtt ttatttggga gttcaggcaa
     7621 gaaaaaacta tttttcaatt tcagcgaata caaaagttac ctcaaactta ctcatctcaa
     7681 aaaagttaat cgaatgggca ctttacggac ctgcggatga aagaaatata aatcaaccgc
     7741 ttgatttctc cgatctgggc ggaagtgtga tggcatacaa cgagtttact ttgggttatg
     7801 gaaggaaatt gcttggcgat cggcttcagg ttggtataag atttaaatac ttaaatggta
     7861 tagccgaagc atcagcaaaa aatattaatg ggtatatcta cgcaggtatc gattccattc
     7921 aattatacag tgaagctttc actatcaaca cctccggaat tgatgatttt gataataatc
     7981 ctgaagactt gattttcggt tttaaaaatc ccggttttgc cattgatatt ggtgcccaat
     8041 accagataaa ttctaaattc tccgtgtcag ctgccttcac ggatatggga tccattaagt
     8101 ggaaaaatag tcttaaaggc tgggaattta atgaggttaa ttatgtattc agaggttttg
     8161 acgtgttgga tgtaattaac gaatccgatg taattgataa cgaattggat tcactggaaa
     8221 atttatatga cattaatgag attgaaggga aagcataccg cgcgccattg gttacaaaga
     8281 cttacctggg tggaagattc cagtttcatc cgaagcaaaa tgttagcctg attctttata
     8341 atgatttctt tcagggaaat gtgaaatctg cccttggttt gaattacggt cttctggtgg
     8401 gaagaagtat ggatctattg gtcggactct cttaccggga caggtcatta gctaatttca
     8461 ctttcggtac tgcatttagt cttgggtttt ttcaattata ttttttatcc gaaaattttc
     8521 catctctctt tatgccgcag aatgcacgaa aactcgacct gcggtttgga attaattttc
     8581 aatttggaag aaaacgaaag ggaagctcct gattattccg caatttgccc tctgcctaaa
     8641 tagcttctaa gtttgggtgg aatttctcgg gttattaaag cattcttgta tctccatttt
     8701 ttaggactct caaattgttt taaaaatttc acttctttcc cttccttcat aacaaccggt
     8761 tcgccgtcct catataatat cctgttagaa gtaattgacg caacccggtg accagtcgta
     8821 ataatacctg ttagattcag cggatctccc gccgaaatgg ctacaagttt tgctttctct
     8881 ttatcgtttc gcgttttgcg catcaaactg accgcttccg gcaaagcata ttgttcaccc
     8941 cagacaccgt ccacaaacct gccgcctctt atttctcctc tcgcctccat ccttcgataa
     9001 acctttaata aatccctcca tggcggagag ccttgctcct tatcaatcag tttcctgaat
     9061 actactccat atcttcgtaa ataaactttc gaaagctgct tggttcgttc ctcttcatta
     9121 attgactcag attcattatt aatgattgac caccgccccg cctgttccat tgaaaacaca
     9181 ttcttacttc ttcgcgactt aaattttgcc ggtgtgagta atgccctcag cccggtatag
     9241 ctatctgatg taactaatcc ggaagcaatt aactccgcaa ttgcttcttc agcctgtaaa
     9301 gccaataaac ctgaactgtt gacgatatcc ttaaagaaag aggcaccatt ttcattcaaa
     9361 atatcgcgga ctaatttccc attcgatgaa agtccggcaa catcagtgag cttgtttttc
     9421 caaataccaa tattgtcgcg ttggattaac atgatgggtg atgccttaat cggactgctc
     9481 atttttgaac cgttatcggt gttaacccga aatctacccc aaaggattcg accagaaaga
     9541 catagcatgt ccagccagaa gaaatcataa tctttaaccc ttaccggtag caaatctcct
     9601 tcccaggctg ccgcgggagc ttcatatccc tccatctgtt ccagcacttt catcaagcca
     9661 tcaggtcctt cctttttgtt ttccacatct acaccctgcc agacaaacaa gaatctcatg
     9721 aaatcggaag aagaaaccgg ttcaatagat ttcctcagcg tttgcaaggt atagcggtgg
     9781 attcttgcca gcaatcttct gtcacaccac tcagtaattt catcattgcc gtggaatgac
     9841 ccgcgcaaaa cgaaaccttc attctctaaa gccattaaag cctgctcgat ttttgttatt
     9901 ggtaattcca ttaactgcac caggtccatg ctttcaactg gccccatcgc ctcgattctt
     9961 ccccgcacaa tttctcgcaa cgcatcctct tcgtcccact tttgacttgc aatcttttca
    10021 gggagattta tttctatttt tgataattcc acagtaaaaa ttctcgcaaa ttgaggaatt
    10081 ctttctgccg caacccaaag ctttcttccc gaattgagtt taatatcaac caccctgtta
    10141 tcagaatgca aaacttccag gaattcattc caggtattgg ccagaatctc ttcctcggag
    10201 aaaaaactaa acagaattaa gctgtcatga agttcatctt cgttaagaac ctgcggccac
    10261 gcttcctctt tcacagcacg aattgcttca ggatccagct tagatagttc gccggcttgt
    10321 tttgggtcca gccaacccct gctttgaaca gcattggttc gcctttcttc cagaggtgcg
    10381 ttatccaaaa aggagtaggg ccgtgcattt ataatttcct gggccattgg agatggtccc
    10441 cttagatctt tccccaccag ggttttcttc ccggatttta tctctgttaa tagtttttcc
    10501 agttcaatga tatccattgc ttcatacaag caatcttcaa tggtttgggt tatcaatgga
    10561 tgatcaggta tttcccgttc cccaacaata ttctcaagac aagctaattg atcaggaaaa
    10621 accagggcca ctaagtcttc ggcttgagaa cgttgaattt gaggcggtac tttcttacca
    10681 cctcttctcc ttagtatagc cagagcgcgg gtcgcattcc atcgccatcg cacctcgaac
    10741 attggtgcat ccagcagcgc ctgaatcaac acatccctaa ccgtctttgg attcagataa
    10801 gaaaaaactt catcaatggc aaagctatga gtagctccca gggaaagaat tatattatct
    10861 tccgtggcgg ctgcctgcaa ctcaaaattg aactttctgc aaaatctctt gcgcagcgcg
    10921 aggccccagg ctctgttcat cctgcttcca aatggggaat ggataacaag gtgcatatct
    10981 cccgcttcat caaaaaagcg ctccatcacc agattatccc gggatggcat tactcccagc
    11041 gctgcctgag tagcggccag gtatgtgact atctggtcac atatttcttc cgggagacta
    11101 atttcggcct ttatccactc cattgcctgg gttttccacg aattatcatc atcctccccg
    11161 attttctccc agtcagggct tcctactaat tccccaattc ttgtacgcaa tctggaaact
    11221 gcttccgata attccctcgt tcttcctggt gcttctccaa accagaatgg aatcgaaggg
    11281 ggggcaccca ttgcatcttc aaccctcact tttcctgttt ccaccttcaa aattctgtag
    11341 gaattatttc ctaattgaaa gacatcaccg ggcatgctct ccacagcaaa gtcctcattc
    11401 agggttccta taaaaagatt ccgaggctcc attattacat cgtaatcaaa attatcaggg
    11461 atagctccgc tgttaatcag tgcggtaagc ctgctgcccc gtcttccctt aagtttccca
    11521 tttatttgat catgatgaac ataagcaccc cggcgtcctc tccgggttgt atacccttcc
    11581 gaaagcattt ggatgatctc gtcgaattct tgcctggtta cttctttgta tggatatgat
    11641 ttccggatga gatggaaaag ttcatcttcc tcataatccc tgcttgacaa ttcagcgaca
    11701 atctgttggg ccaggatatc cagcggttta accggaataa tgatttcatc cagttcaccc
    11761 aatttaattg aatgaaggag agctgcgcac tctatcattt catcccggct gagcgggaag
    11821 attattcctt taggagttcc gtttaccgtg tgcccggaac gaccaacacg ttgcaaaaac
    11881 ttcgcgatgg atttcgggga tccaatttgg caaaccaaat ctattcctcc aacgtcgatt
    11941 cccaattcca ggcttgcggt tgcaattacg gcttttaatt tacctccctt caacctattt
    12001 tcagcatcga gtctatgttc cttcgacatg cttccatggt gcgccgttac cgttccttct
    12061 cccagtcgat tagacagatt atgagacatt cgttctgcca agcgtcttgt attcacaaaa
    12121 ataagggtag tattatgcgc ggtgataagc tcttctacgc gatcgtagat ttcttcccac
    12181 acttcattgg ccattacagg cgccaacggg gatctgggta ctccatcttt aattcaagag
    12241 gccgtttatg tcccgcatta attatactgc atttccgcgt attgttgcca acaagaaaat
    12301 cagctacttt ctcaatgggt ttttgtgtgg ctgaaagtcc aatcctgacc aaagatttat
    12361 ccgccaattt ctcaagtctt tcaatggata aggaaagatg cgaacctcgt ttatctccca
    12421 gcattgcatg aatttcatca acaattatag tccgcgttgt agaaagcatt ttacggccgt
    12481 tctcactggt aagcaataga tataaagatt caggcgtgct aactataatg tgtggcggtc
    12541 ttttaatcat cgcggtcctt tgggcgggtg tagtatcacc cgttctcagt ccaactctca
    12601 cttcaacttc ttttccttcc cttttataat acctggaaat tccatttaaa ggagtctgaa
    12661 gatttttttc gatatcattg cttagagcct tgagtggaga cacatatata acctgggtct
    12721 catcttcaag atttccatcg aggctttgaa cgaataaatt atcaagaata gaaaaaaatg
    12781 ccgacaaggt ttttcctgaa cctgtaggtg ctgagattaa aacattattt gaacttttaa
    12841 ttaccggcca cgcttttgtt tggacctcgg agggctcgcc aacattttct ttaaaccatt
    12901 tccaaacaat tggattgaat agcgattgca gattttgatg atccggcttc atgtattcta
    12961 atgtacgtaa cttcctgcat taatgtctat ggttgcgccg gtggcatgat ttgccaaacc
    13021 gctggcaaga aaaagtactg tgggcgcaat gtctttaggt tctgttaatt cagataatgc
    13081 tatatcacca gtagcataat cattaccata tttatctatg aaaacctgag ccatttcggt
    13141 gcgcgtaaaa cctggagcaa ccgtaaa
//
LOCUS       DASWHX010000152         6051 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_3122329, whole genome shotgun sequence.
ACCESSION   DASWHX010000152 DASWHX010000000
VERSION     DASWHX010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 6051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6051
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3122329"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(73..354)
                     /locus_tag="VGA21_10275"
     CDS             complement(73..354)
                     /locus_tag="VGA21_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009195756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEX9650937.1"
                     /translation="MAKIKWGKRAVNDLKAIHNYIFPDSQFYADRYIDKLLSRVDQLE
                     SQSKLGRIVPEKGDSTIRELIEGNYRIFYRVYKNDVFILRIHHAARNIK"
     gene            complement(342..521)
                     /locus_tag="VGA21_10280"
     CDS             complement(342..521)
                     /locus_tag="VGA21_10280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650938.1"
                     /translation="MKKAAVISALNEFPKEFDLEDFLERLIVINKIDEGLKDIKEDKI
                     VSHSAVKKMVKKWQK"
     gene            complement(1193..1687)
                     /locus_tag="VGA21_10285"
     CDS             complement(1193..1687)
                     /locus_tag="VGA21_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014019266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650939.1"
                     /translation="MSFENRISLKISADDQTEITTAITTLVNKLGPHLKALSAEDRRQ
                     LVKMGDSNVPFVEKALDYALSNPEFAPPYLKVDELMIDVEGVTLLTNYLRLLEQMVSN
                     LKDSITLAGSEAMAGALTYYNSCKQAAKMNVPNAKSILDDLAKRFKGQGRTHEEVLPE
                     EPVQ"
     gene            complement(2290..2805)
                     /locus_tag="VGA21_10290"
     CDS             complement(2290..2805)
                     /locus_tag="VGA21_10290"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEX9650940.1"
                     /translation="MRANLIIILICLVAESSAQELVKLSGEQRQEFIDVHNQWRANVG
                     TKPLEWNNDLAKYAAEWAMQLTKKCQMEHRPTEGKWKRIYGENLYWISTSSASPKHAV
                     DSWGSEIQYYKKPAPIKSPNKYGHYTQMVWKNTTEVGCAIAVCKEGGTLVVCNYDPPG
                     NWLGETAYDKQ"
     gene            complement(2899..4515)
                     /locus_tag="VGA21_10295"
     CDS             complement(2899..4515)
                     /locus_tag="VGA21_10295"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009184642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HEX9650941.1"
                     /translation="MKYILLLSVFLTFGCSAPRGEQPETQRPNIFLIVADDLGYSDLG
                     IYGGDISTPNIDELAKSGLLFTRFHTAVMCAPTRAMLLSGNENHLAGVGSQSGGTGIF
                     AGKFGYEGYLTNRIVPFPLLLHDAGYHTYITGKWHLGRTRETSPAAKGFERSWVMEDG
                     AGNHYNDVSLFDNDSSTYREDGELVNYPEGEYSTEFYTNKLIQYIEGNLNDGKPFFAF
                     AALTTPHWPLQVPQQFDNYKGRYDPGYDSLRCLRFQSLKKAGIIPEQAKFPERLEGII
                     PWNELSEEQKKIEARKMELYSAMVENLDYHVGRMIQFLKDKNLFENTLIVFMSDNGAA
                     AEDFYSKLREGSDLYGYYDNSYENMGKPNSFVSYGPPWAMAGSAPFNRHKSYPTEGGI
                     SAPLIMRGVNIPANGEIRHDYLTVMDLAPTFLEIAGIGYPELQGTENTPPMAGVSLLP
                     FLKGENTIVHDENYVTQHEHRLRLYVRKGDWKLVSIDLPVKEENFRLYNLATDLAESK
                     DLKQEFPEVYNELLTEWHKYVEEARIIIPE"
     gene            complement(4541..4999)
                     /locus_tag="VGA21_10300"
     CDS             complement(4541..4999)
                     /locus_tag="VGA21_10300"
                     /inference="COORDINATES: protein motif:HMM:NF039391.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6265 family protein"
                     /protein_id="HEX9650942.1"
                     /translation="MKQIVNSILLLVIWASAFAQEKDIDQFSWMLGEWRGAQGQAGLY
                     EKWDKIDESLMLGEGYYIVNGDTVIREILRIQKIGRFWTYIPIINKGKPVMFTLIESE
                     PGVWIFENKEHDFPQRVVYSQKTDGSLFAWIEGEQKGQFMKDEYAMQKVN"
     gene            complement(5004..5372)
                     /locus_tag="VGA21_10305"
     CDS             complement(5004..5372)
                     /locus_tag="VGA21_10305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002703380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX9650943.1"
                     /translation="MQNFINWFEIPASDIKRAVKFYQEILQIEITETEMMGMKMGFLP
                     NDGKNVSGAIVQGEDYSPRTDGTLVYLNGGHDLDLPLKRIEPAGGKVLVQKTQISPEI
                     GFFAMFIDPEGNKVALHSIN"
     gene            complement(5459..>6051)
                     /locus_tag="VGA21_10310"
     CDS             complement(5459..>6051)
                     /locus_tag="VGA21_10310"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX9650944.1"
                     /translation="TTFIQIGPSGKTGIIAARFYPHGANAFLNFPVSEITNHSVDMRS
                     LFGIEGGELEERIISANTNPERIQILQDFLIKKLYQYQKDDTVIIECIREITATKGDI
                     KIESLSQNLNISQRQLERKFASTVGLNPKYLGRIIRFNNIFKLVQKKEINSLTMLSHE
                     SGYYDQAHFIRDFKQFTGLNPKVYFRGEHSLSKLFV"
BASE COUNT         1650 a         1285 c         1156 g         1960 t
ORIGIN      
        1 tgtcttatga cttttcactt atgacttatg acttatgact gtttgccagt catgttagct
       61 aaaaatcaat ctctacttaa tgtttcttgc ggcgtggtga atacgcaata taaatacgtc
      121 attcttgtaa actctataga agatacggta attaccctca atcaattcac gaatggttga
      181 atctcctttc tcaggaacaa tacgtcctaa tttggattgt gattccagtt gatcaacgcg
      241 ggataaaagc ttatcaatat atcggtcagc gtaaaactga gaatctggga aaatataatt
      301 atgaattgct ttaagatcat taacagctct ttttccccac tttatttttg ccatttcttc
      361 accatttttt taacggctga gtggctgacg atcttatctt ccttaatgtc ttttaatcct
      421 tcatcaatct tgttaattac aattaatctt tccaggaaat cttcaagatc gaattctttt
      481 ggaaattcat taagagcgga gataacagct gcttttttca taattcaaat atactaaata
      541 tttgaacacc ttgaccagcc gggttttgca acaaggttta catttgtttg atttccggta
      601 gaaaccggga ttgactttcg acttttgact tttgactttt gacttttgac ttttgacttt
      661 tgacttttca cttttgactt ttcacttttg acttttcact ttcgactttc gactttcgac
      721 ttttgacttt tcacttttga cttttcactt ttgactatcc tgagacaact ggttattgcc
      781 ggaaaccaac tgacgataca ttcacaacaa tcggtgggtc ttcggcacca actaattatt
      841 aatagaagtg aactaacgga tactaattac caactggtgt attacggtga atagctggtt
      901 atatccggag aataactgac ggaagctgga taccctctgg ttatgtcccg gatataacag
      961 gttggtgctt caggataact aattatgtaa aaagtataac cggttatact ctgcatatcc
     1021 attggggggt acagataccc tatggggggg gtaccacgat acccctgggg gtacctgaca
     1081 tcccccaaat aaggtgttta aggcaggcag ggacagtaaa agacaaaaga ggcattcaaa
     1141 aaaaagtcgg attttcatcc gacctcaaca aagtataaat catgataatc tattattgta
     1201 caggttcctc tggcaatact tcctcgtgcg ttcttccctg gcctttaaat ctttttgcca
     1261 ggtcatctaa aatacttttt gcattgggca cattcatttt ggcggcctgt ttgcaggagt
     1321 tatagtaggt gagagccccc gccattgcct cactgccagc cagggtaatg ctatccttca
     1381 ggttggatac catctgctcc agcaaacgca aataattggt cagcagagta acgccttcca
     1441 catcaatcat caattcatcc actttcaaat aaggcggtgc aaattcagga ttgcttaagg
     1501 catagtcaag tgccttctcc acgaaaggca cattgctgtc acccatttta actaattgac
     1561 gtctgtcttc cgctgacaga gctttcaaat gtggccccag cttgtttacc aaggtagtaa
     1621 tggccgttgt gatctcagtt tgatcatcag cggaaatttt tagggatatt ctgttttcga
     1681 agctcataga tagtaaatgt ttattgtttg caattatttt tttgtaataa tggtgaattt
     1741 tgttattccc gccttggagg attgtaacaa aatctggtag ttatgtaacc tggatgacat
     1801 tttagagaac aggtgcttaa tttcccctcc ttattttaag gagggcctgc cgaccgaagg
     1861 ttggttaggg gtggttaata attttcttat agtgtctctg aaaatagaaa tcagttgtta
     1921 tatataaccc cctgtagtcc cccttgaaat aagggggaca atttctgggc agggctaaat
     1981 ttctcctcct tattttaagg agggggcagg ggtggttaag gctggtttct gaaaatagaa
     2041 atgcagttgt tatatgtggg agggaaaata ccccctgtag tcccccttaa aatatttact
     2101 taatttcccc tccttatttt aaggaggggg taggggtggt taataaaatg gtaattatac
     2161 cccatatagt cccccttaaa acaaggggga caattttcgg gtgggggtaa aaaatatacc
     2221 ggttttgcaa ctatgtttac atttgcttga tttcccgcca gaaaccggga tgaaattcta
     2281 cttcactgtt cactgcttat catacgcggt ttcgcccagc caattccccg gtggatcata
     2341 attgcagacg acgagggtgc cgccttcttt gcatactgct atagcacagc caacctccgt
     2401 tgtatttttc caaaccatct gggtataatg accatacttg ttgggcgatt ttatcggtgc
     2461 gggtttttta taatattgaa tttcactgcc ccaggaatcg accgcatgct ttggagaggc
     2521 ggatgaagta gagatccaat acagattttc tccatagatg cgcttccatt ttccttcggt
     2581 ggggcggtgc tccatctggc atttttttgt taactgcatg gcccattccg ccgcgtattt
     2641 tgccaggtca ttattccatt ccaaaggttt cgtgcccaca ttggcacgcc actgattatg
     2701 aacatcaata aattcctgcc tttgttcgcc tgaaagtttt accagctcct gggctgaact
     2761 ttctgctacc aggcagataa ggataatgat taagttggct ctcataacgg ataaaatata
     2821 attaatgaat attttctgca agaataaggc cgaataggga tcaattacca aatttatccg
     2881 gcagtagtaa ggataatgct attcgggtat tataattctg gcttcttcca catatttatg
     2941 ccattcagtg agcagctcgt tatacacttc agggaactct tgtttcaaat ctttggattc
     3001 tgccaagtct gtagcaagat tatacaaacg aaaattttct tcctttaccg gcaggtcaat
     3061 actcaccaac ttccagtcgc cctttcttac ataaagccgc aggcggtgtt catgttgggt
     3121 tacataattt tcgtcatgca cgatagtatt ctcgcctttc agaaatggaa gtaaagatac
     3181 gcccgccatg ggcggagtat tttctgttcc ttgcaattcc gggtatccaa ttccggcaat
     3241 ttcaagaaat gtcggggcaa ggtccataac cgtgagataa tcatgcctga tttcaccatt
     3301 ggcaggtata tttactccac gcataattaa tggagctgaa attcctcctt ccgtagggta
     3361 gcttttatgc ctgttgaatg gtgctgaacc tgccatcgcc catggcgggc cataactcac
     3421 aaacgaattc ggtttgccca tgttttcata cgagttatca taatacccat acaaatcact
     3481 accttccctt aattttgaat aaaaatcttc tgctgctgct ccattgtcag acataaacac
     3541 tatcagtgta ttttcaaaaa gattcttatc tttcaaaaac tgaatcatac ggccaacatg
     3601 gtaatcaaga ttttcaacca tcgcggaata taattccatt tttctggctt caattttctt
     3661 ctgttcctca ctaagttcat tccagggtat aatcccttcc aggcgttcag gaaacttagc
     3721 ctgttcagga ataattcctg cttttttcag ggattgaaag cgaagacagc gcaagctatc
     3781 ataaccagga tcatatctgc ctttataatt atcaaattgt tgtggaacct gaagtggcca
     3841 atgtggagtg gtcaatgctg cgaaagcaaa gaaaggtttt ccatcattca gatttccttc
     3901 aatatattgt atcagcttat ttgtatagaa ttctgttgaa tattcacctt ccggataatt
     3961 caccaattca ccatcttccc tataggtgga agaatcattg tcaaaaagtg aaacgtcatt
     4021 gtagtgatta ccggctccat cttccatcac ccaggaacgt tcaaatcctt ttgccgccgg
     4081 acttgtttct ctggtccggc ccaaatgcca tttgcccgtg atatatgtgt ggtaacctgc
     4141 atcgtgtaat aacaatggga atgggacaat ccggttagta aggtatcctt catatccaaa
     4201 tttgcccgcg aaaattcccg ttccaccaga ctggctgccc actccggcga gatgattttc
     4261 atttccactt aatagcattg cgcgggtagg agcacacatt actgctgtat gaaaccgggt
     4321 aaacaggagt cccgatttag ccagttcatc aatattagga gtagaaatat cacccccgta
     4381 aatgcccaga tcagagtaac ctaaatcatc agccactatc aggaaaatgt tcggcctctg
     4441 tgtttcaggt tgttcacccc tgggagcaga gcatccaaaa gtcaaaaata cacttagaag
     4501 gagaatgtat ttcatccctc taatataaaa aggaattgaa ttagtttact ttttgcatcg
     4561 catattcatc tttcatgaat tgtccttttt gttctccttc aatccatgcg aagagtgaac
     4621 catctgtttt ttgagaataa actacccgtt gcgggaagtc gtgttcctta ttttcaaata
     4681 tccaaacacc cggttccgac tctatcaggg taaacatcac gggctttcct ttgtttatta
     4741 taggaatata ggtccagaat cttcctattt tttggattct taatatttca cgaatgaccg
     4801 tatctccatt gacaatataa taaccttcac caagcatcag ggactcatca attttgtccc
     4861 acttttcgta aaggcccgcc tgtccctgcg ctccccgcca ctcgccgagc atccaggaga
     4921 attgatcaat atctttttcc tgcgcgaatg cagatgccca aattaccagg agaaggattg
     4981 aatttacaat ttgtttcata aagttaattt attgaatgta aggcaacctt gttaccttcc
     5041 ggatctataa acattgcaaa aaatcctatc tccggagaaa tttgcgtttt ttgaactaat
     5101 actttgccac cggcaggttc aattcttttt aagggaagat ctaaatcatg acccccgtta
     5161 agatatacga gtgtaccgtc ggttcgggga gaataatctt ctccctgcac aatcgcaccg
     5221 gaaacgtttt taccgtcatt aggtaaaaat cccattttca tgcccatcat ttctgtttca
     5281 gtgatctcaa tttgcaaaat ttcctgataa aatttcacag cccttttaat atctgatgcc
     5341 ggtatttcaa accaatttat aaagttttgc atagttgctt tgcatttaat tttacaattt
     5401 cccgctaatc taagcctcgt gaattaatca aaattgtaaa aaaccgacag gataaatttt
     5461 agacgaaaag ctttgacaat gaatgttctc ccctaaaata aactttgggg ttcagtcccg
     5521 taaattgctt aaaatctcta ataaaatggg cctggtcgta ataaccactt tcgtgcgaaa
     5581 gcatcgttaa gctgtttatc tctttttttt gcaccaattt aaaaatgtta ttaaagcgga
     5641 ttattctacc caggtattta ggatttagtc cgacagtgga tgcgaacttt ctttccaact
     5701 ggcgctggct gatattaaga ttttgagaga gcgattcaat cttaatatca cctttggttg
     5761 ctgttatttc acggatgcac tctattataa cggtgtcatc cttttggtac tgatagagtt
     5821 tcttaatgag aaagtcttgc aggatttgaa ttctttccgg gtttgtattt gctgagatta
     5881 ttctttcttc aagttccccg ccttcaattc caaacaggct tctcatgtct acggaatgat
     5941 ttgtaatctc actaactgga aagttgagaa acgcattggc tccatgtgga taaaatctgg
     6001 cagcaattat cccggtttta ccgctggggc cgatttgaat aaaggtagtg a
//
LOCUS       DASWHX010000153         3695 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_3130518, whole genome shotgun sequence.
ACCESSION   DASWHX010000153 DASWHX010000000
VERSION     DASWHX010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 3695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3695
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3130518"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..550
                     /locus_tag="VGA21_10315"
     CDS             <1..550
                     /locus_tag="VGA21_10315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009579881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEX9650945.1"
                     /translation="AHGIRNTVGFDWHPGTGELWFTDNGRDMLGDDIPPCELNKVSVQ
                     GQHFGYPYCHGGRYRDPEFGIERTCDEFVNPELNFIAHTAPLGMRFYSGNMFPSSYRN
                     QILVAQHGSWNRSTKSGYKVLLVTVENNKAVKDEIFIEGWLNEATQAVWGRPVDILQL
                     EDGSILISDDHAGLIYRVTYKG"
     gene            complement(624..1124)
                     /locus_tag="VGA21_10320"
     CDS             complement(624..1124)
                     /locus_tag="VGA21_10320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650946.1"
                     /translation="MKKVILVFLFGIITHQSKAQLDSLTFDFWVGKWAATWQNSKGET
                     ETGTNLIEKILDERVLQEHFEILSGGNKGFKGTSISVWNSRNKTYHQAWADNQGGYFN
                     FTGEVDSDKKIFKTSPRARGDQTIIQRMVFYDIKENSFTWDWESSTDNGDTWSLNWRI
                     NYSRSN"
     gene            1468..3444
                     /gene="ispG"
                     /locus_tag="VGA21_10325"
     CDS             1468..3444
                     /gene="ispG"
                     /locus_tag="VGA21_10325"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015265930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(E)-4-hydroxy-3-methylbut-2-enyl-diphosphate
                     synthase"
                     /protein_id="HEX9650947.1"
                     /translation="MVEKTLLQQNFRYCESLTRYSRRKTREVYIGDLPVGGINPIRLQ
                     SMTIVDTMDTQGSIEQSIRMIEAGCEYVRITAPSLNSAKNLKNIKAGLRARGYNTPLI
                     ADIHFTPQAAELAATLVEKVRVNPGNYADRKKFQQIEYSDADYAAELRRIRERFSPLV
                     KICKDHGTAMRIGTNHGSLSDRIMSRYGDTPLGMVESALEFVRICEDLGYYDLVLSMK
                     ASNPQVMVQAYRLLVNRLEAESLQPYPLHLGVTEAGEGEDGRIKSAAGIGLLLEDGLG
                     DTIRVSLTEDPEFEIPVARILADRYKNRSAHKPIPGLEVSPKNPFEYFRRESHEVFNL
                     GGTNVPRVIIDISEKKELSMKDLASIGHFYLPEPDKWIMNDTGSDFIYSGNTTINFML
                     PNGLKEIVDYNGWLMRDDKSESFPLFELEQISDAVFSPQTNFIRIESKNAGDDFFNFI
                     KSQLNFLIILETSNLHGLADQRRFFFELIQRGITNPVIIKRDYNHLSSEEFICHASTD
                     AGGLFIDGFGDGIWLSLNGNDHGWSDKIKLINRTSFGILQAARTRTTKTEYISCPSCG
                     RTLFDLQKTTAMIRKRTDHLKGVKIGIMGCIVNGPGEMADADYGYVGSGRNKITLYKG
                     KEVIKRSVTAEHAVDELIEIIKNDGNWIEPASVN"
     gene            3449..3661
                     /locus_tag="VGA21_10330"
     CDS             3449..3661
                     /locus_tag="VGA21_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017729931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6728 family protein"
                     /protein_id="HEX9650948.1"
                     /translation="MEVNEQSPKEKNALRDYFKLGEVGAYFFRKKDPTRPTNFNIKVM
                     HGINRISILVFLGALIIFLIRRFLMG"
BASE COUNT         1102 a          697 c          810 g         1086 t
ORIGIN      
        1 tgcgcatggg attagaaata cggtagggtt tgactggcat cccggcacgg gagaattgtg
       61 gtttaccgac aatggccggg atatgctagg agatgatata ccgccctgtg aattgaataa
      121 ggtgtcagtt cagggtcagc actttggtta tccatattgt catggcggca gataccgtga
      181 tccggaattt ggcattgaaa gaacatgtga tgagtttgta aacccggagt tgaattttat
      241 tgcacacaca gccccactgg ggatgagatt ttattccgga aacatgttcc cgtcaagtta
      301 ccggaatcaa attctggttg cgcagcatgg ttcatggaat cgatccacaa aaagcggata
      361 taaagtgtta ttagtaactg tagaaaacaa taaagcggtg aaagatgaaa tctttatcga
      421 aggatggttg aatgaagcca cgcaggcagt ttgggggcgg ccggtagata ttcttcaatt
      481 agaagatgga agtatcctga tctcggatga tcacgccgga ttgatttaca gagtaacata
      541 taagggttaa ttatggtaag tgacaggtta ctaacacaac ctgcaattta ctttgacatg
      601 accacttaac tgctagctgc aaattaatta cttctcgaat aattaattct ccaattcaaa
      661 ctccaggtat ctccgttatc tgtgcttgat tcccaatccc aggtgaaact gttttccttt
      721 atatcataga aaaccatgcg ttgaataatt gtttggtcac cacgcgctcg tggtgaagtt
      781 ttaaagattt tcttgtcgct gtctacttct ccggtgaaat taaaatagcc accctggttg
      841 tcggcccacg cctggtgata agttttgttt cgggaattcc atactgagat tgaagtccct
      901 ttaaaccctt tgttaccacc ggaaagaatt tcaaaatgct cctgaaggac tctttcatcc
      961 aaaatcttct caatcaagtt agttcctgtc tcggtttctc cctttgagtt ttgccacgtg
     1021 gctgcccact tgcctaccca aaaatcaaag gttaaagaat ccaattgtgc tttgctctga
     1081 tgagtgataa ttccaaatag aaatacaaga atgacctttt tcatagctct ttaatttaat
     1141 ttttgattga aaatgaaacg atttcgattt acaaaagtaa aatccgatag gaatttacct
     1201 ttaataggta gcttttccgt atttacctta cccttttgca gacaataatg gctagtagtg
     1261 atttctgaat tactgtgaat tactgatttc gctcaaactg aaataagaaa agcatataaa
     1321 gtgtttttta attattattt tgaaacgctt tcaattattt aaccattcct gaagatgggc
     1381 taaggctgag atcatacttc ggagcatgtt ttttccaact caaatcaagt atttagcaat
     1441 aagctccctt acctttgtat tcatttgatg gtcgaaaaaa cactacttca gcagaatttc
     1501 agatactgcg aaagtcttac ccgctattcg agacgtaaga cgcgtgaagt atatatcggt
     1561 gatcttcccg tcggcggtat caatcccatc cggctgcagt cgatgacgat tgttgatacc
     1621 atggatacgc aaggttctat tgagcaatca atccggatga ttgaagctgg ttgcgagtat
     1681 gtgcgtatca cagcaccaag tttaaattca gcaaagaatc tgaaaaatat taaagcagga
     1741 cttagagcca ggggatacaa cacgccacta attgcagaca ttcatttcac gcctcaggca
     1801 gcagaacttg cggcaacatt agtggagaaa gtgcgggtca acccgggtaa ttatgccgac
     1861 cggaaaaaat ttcaacaaat cgaatattcg gatgcagact atgctgcgga gcttcgaaga
     1921 atccgggagc ggttttctcc gctagtgaaa atttgcaaag accatggaac ggccatgcgc
     1981 ataggtacaa atcatggaag tctgtcggac cgaattatga gccgctatgg tgacactccg
     2041 ctgggaatgg ttgagtctgc cctggagttc gtaagaattt gcgaagacct cggctattat
     2101 gatcttgttc tatctatgaa ggcgagtaat cctcaggtaa tggtgcaagc ctacagacta
     2161 ttggttaaca ggcttgaagc tgaaagtttg caaccctatc cattgcatct tggagtaact
     2221 gaagccgggg aaggagagga tggaaggata aagtcagcgg caggtatcgg attgctcctg
     2281 gaggatggtc ttggagatac aatccgggtt tccttaacag aggatcccga gtttgaaatt
     2341 cccgtcgcca gaattttggc ggatagatac aagaatagat ctgctcataa accgattcct
     2401 ggcctcgaag tttctcctaa aaaccctttc gaatatttca ggagggaatc gcatgaagtt
     2461 tttaatttgg gtggaacaaa tgttcccagg gtcatcatcg atatttcaga gaaaaaagaa
     2521 ttatccatga aagacctggc ttccattgga cacttttact tgcctgaacc agataagtgg
     2581 atcatgaatg ataccggcag tgatttcatc tatagtggaa atacgaccat taatttcatg
     2641 ctgccaaatg ggcttaagga gattgttgac tataatggct ggctgatgcg ggatgacaaa
     2701 tcagaatctt ttcctttatt tgaattggaa cagataagcg atgccgtttt ttcaccccaa
     2761 acaaatttca tcaggattga atctaaaaac gcgggagatg acttttttaa cttcataaaa
     2821 tctcaactaa attttttaat cattctggag acttcaaatc tgcatggtct ggctgatcag
     2881 cgaagattct ttttcgaact cattcaaaga ggaattacga atcctgtcat tataaaacga
     2941 gattacaatc atttgtcctc agaagaattt atttgtcatg cttcgactga tgccggtggc
     3001 ctttttattg atggttttgg agatgggatc tggctaagcc tgaatggaaa tgatcatggc
     3061 tggagtgaca aaataaaatt aattaaccgc acctcctttg gaatactgca ggccgctcgt
     3121 acccgtacca cgaaaacaga atatatttcc tgtccatcct gtggaagaac cttatttgat
     3181 ttacagaaaa ccacggctat gatccggaag agaaccgatc atctaaaggg agtcaaaatt
     3241 ggaatcatgg ggtgtatcgt taatggcccg ggtgaaatgg cagatgcaga ttatggttat
     3301 gttggttccg gcagaaataa aattacgtta tataagggta aggaagtaat taaacgatct
     3361 gtcactgctg agcatgccgt tgatgaatta attgaaatta ttaaaaatga tggaaactgg
     3421 attgagccag catctgttaa ttaaaattat ggaagtaaat gaacaaagtc cgaaagagaa
     3481 aaatgctctt agagattatt ttaagttagg tgaggttggc gcatattttt tccgaaaaaa
     3541 agacccaacc cggcctacca atttcaacat taaagtgatg catggcatca ataggatttc
     3601 cattctggtt tttcttggcg cattgattat tttcctaatc aggagatttt taatgggatg
     3661 aatattgaag aattccggtc attttgcctt tctct
//
LOCUS       DASWHX010000154        12969 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_152124, whole genome shotgun sequence.
ACCESSION   DASWHX010000154 DASWHX010000000
VERSION     DASWHX010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 12969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12969
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152124"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(212..2629)
                     /locus_tag="VGA21_10335"
     CDS             complement(212..2629)
                     /locus_tag="VGA21_10335"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9650949.1"
                     /translation="MISNYIKIALRYLFRNISSSSLNIIGMSIGIACVLVIVAHVQFE
                     LSYDTFHSNHKNIYRLSEKRIDEGRELNSAVSFSPLAELMRSHIPSITRVVRMYPITG
                     IISPDKINKEKETLFTFADSLLFDVFDFIAVHGDLNTALNHPFSVVLSESRAIKMYGK
                     SDVVGRSLYFEDEEDLYEFTITAIIKDIPQNSHFNTNILASFASISKIQPWYNNWHYP
                     PMFIYVEVKDGTNPNDLQTQIKSMGENHFPDYIKTEKREYIPQNTSDIHLFSDLESEW
                     QANSNYEYIKFFMIIAGFILVIACINFTNLSTVQSIKRSHEVGLRKVLGASKLQLVGQ
                     HLGESLIITLISVVISFGLAELILVNFYNQLIGKTISIGFLLEGVNLVAVIASIVLVI
                     LLSGSYPAFYLSKFSPVSALTNKGNLKSGLGSFSKFLVVFQFIVASILIIATITIQRQ
                     ISFMRTTNLGFNEEQLIAIRLVDRESQVKYQVLTDELLKESMVENVALSSTIPGVVSY
                     FVEWQILSEEMPEGTEFSMASLGVDEEFIPTYNIEILEGRNFSKDIPTDLKAGYILNE
                     AAADKLGWENPVGKDFQMTVYINGEEVRKGKVIGLVKDFHHESLYNSIDPLVLYINKH
                     PYFSDYLTIRLNHTNWQDAIAMLESKWKSFNPDKPMELVFVDDEIEKNYNSEVLMGKI
                     FGAFSVLSIIISCLGLFGLSSYTAQLKTKEIGIRKVLGADITSILKMLYREYLGLVLI
                     ANLISWPVSYYILNKWLTTFPYHVNIGVLVFILALIIAALITIAAISLQSMRSATANP
                     VKSLRTE"
     gene            complement(2635..5031)
                     /locus_tag="VGA21_10340"
     CDS             complement(2635..5031)
                     /locus_tag="VGA21_10340"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9650950.1"
                     /translation="MLKNFIQFSWRKLMKDKSFTSLNLMGLSLGVAFSVIIFLLISYL
                     TGFDTFHKNPDRIYRVITETLQNDNMHYSSGVPVPLGPRMQEDFAEIEKITSVFYDVG
                     GLITITESDGSFSKYNENSGIAYLDMAFYEIFSRNVLQGDLKATLKQPDHVALSKKMA
                     EKYFGEGTPIGKIINLNNSKDLVVGAILEDYSNNTHFPFEVMISYETYRRDNSSNMDN
                     WNSNASDYQTYFLLKENSTAPSLSQKLPEIVEKYHADRPSGTLRYLLQPLADLHFDTR
                     TSNYLYKSVPKSSILTMSIVGFLLLLTACINFINMATAQAIKRSREVGVRKVLGSSRV
                     QLAIRFLGETALICLLALIVGLGLAELFLIEFNALLEVDLHIDQSGPGMIVFLFAAWL
                     AMSFMAGLYPAMVMSGFSPIRALRSSISGGRGGSLNFRRGLVVFQFLVSQLLIISAII
                     IMTQTNYLKNAPMGFKKDLIVVTRLPEQNASKLSVLRNSLLSNSNVQNVSFGFSPPAS
                     GSVWGTYMKFPQTLGEENISVHVKMVDSSYLSTYELKLRAGRFLENSDTARNVVINET
                     LAKMLVRDEPESLLGQNILMWGRILPIIGVVEDFHATSLSEPIAPLIFTNTSSRYHVA
                     GVKINSENVSETLSQIESAWKEVFPQYVFDYTFLDEAIALFYRSQEKMSDTLLLFVAV
                     TLFIGCLGLYGLISFMVNNKTKEVGIRKALGASTTQVMKIFVNELVILVVIAFVIAAP
                     IAHILIGDWLNQFAYRIEIQAWMFIVTIVLVIIITFITVGYKSLRAAQANPVESLRTE
                     "
     gene            complement(5059..7440)
                     /locus_tag="VGA21_10345"
     CDS             complement(5059..7440)
                     /locus_tag="VGA21_10345"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9650951.1"
                     /translation="MIQNFFKTAVRNFLRNSFYSAINVFGLSIGITCAILIFSIIRFE
                     LSYDEDQDAENIYRVITEQVSYGELGSDTGVPNPFTSAFRDDFPEAKYVTIIDGSFRN
                     PVFSVDNNSGISKFFEAPVYVDPDFFKMFRLKWIEGSPEAAHPNKNSVVISRSVANKY
                     FGRTNVVGETILQDSEDELTITGVAEDFPENSSFDFTIFLPYAAKFDEPNNNWNSTSS
                     ATQCFLTLHEDADPEDVEYRLTDFLFKYKAKGANEERTRLYLQKLSEVHFDTNIGFTF
                     TRIVEERSIYALGLIGVFLILTASINFINLNTVQVVKRAKEIGIRKVMGGRRSGLILQ
                     FFSETSLITLASVFLSLGLVELSLIHIDKLLGYSLEFGSWLQPGTLIFILVLFVTITI
                     LSGWYPAMVLSGFQPAQALKKKLASSSKSSLGLRRVLVILQFVISQVLIVCTIIVGMQ
                     MNYFNNTPMGFNKEAIIEVKLQGYEKTQLQSFKNELLEQTAIKNLSYSNTSASSGNIW
                     GGSFRYYKGDEEIREEAQVKWVDHDFAKTYGLEIIAGEDLLPGDTTNSFLVNEEFLNA
                     IGASSPEEVIGNFINMWGEQAPVMGVVKDFFSASMHEKITPVVIGIRNQYAMAGIKVS
                     TNDLQGTLVKIEQIYNKVFPRSIFEYDFLDDRIKEYYEDEQRESILFSTFSIIAIAIG
                     CLGLLGLVSFMAQQRVKEVGIRKVFGASIAHLLGLFTREFVVLILIAFILATPVSYFA
                     MEAWLADFPYHIDINVWVFILAGGISIFIAVFTVGYKSYSAASANPVDVLRDE"
     gene            7600..8973
                     /locus_tag="VGA21_10350"
     CDS             7600..8973
                     /locus_tag="VGA21_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020530658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX9650952.1"
                     /translation="MERQEGSLLIVDDDRDVLETARMFLKQQFEQVIIEPNPNAIPRH
                     IEEFNPDVILLDMNFRKGMNDGEEGFYWLKQILDKDENAVVILITAYGEVDLAVKAIK
                     MGAIDFVLKPWKNQKLLGTILSGLKLRKSKLEVGRLRTTQRKLEDNISRQFGNFIGES
                     PVMQRVFELINKVADTDANVLILGENGTGKELVARALHNKSSRKNNGFISVDLGAVPE
                     NLFESELFGHTKGAFTDAKDNRVGSFELASGGTLFLDEIGNITPPLQAKLLAVIQNRK
                     IKRVGSNKDINIDIRLICATNLPLYEKVRDGSFRQDLMYRINTVEIMLPALRERSEDI
                     ALLTRHFADLYCKKYMKPTMKVSKETISRLQNYNWPGNVRELQHAIERAVILAEGNSL
                     QSSDLLLREPTLESKPFFKATTLEEMEKKFILQSLKDNQGNVTQTARTLGLTRTALYR
                     RLNKYGL"
     gene            8974..10341
                     /locus_tag="VGA21_10355"
     CDS             8974..10341
                     /locus_tag="VGA21_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009583558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9650953.1"
                     /translation="MVLKRFSLLVILRIILILGNVIVIAFILGDARLFFNQIILFIFL
                     TAQIADLIRYVNKTNRELVKFLNAIKQSDFSMTFSQNSAGKSFSELFQAFETIINSYK
                     KVKIEREAQYLFLQKLVDHIHVGIIAVENKDKLVLLNNSARKLLEIPELRNWKGLEAR
                     TPDLIREVKLLEPDGRKLAGIAVGNNKKNLSLDVSSIQLIGEDTILITFQDIKSEIEQ
                     TEIEAWHKLIRILTHEIMNSVTPISSLTETMQMVLRKEDRIRKAEEINDETIEDLAFS
                     LETIQKRSDSLLSFVDSYRKLTKVPRPILGQVNSKELLEASVKLLQAEINKNNIEVSI
                     EFDDPPIEIYLDRSLVEQVLINLITNAIHALEGIANPKLILRSYANENRKVIQITDNG
                     VGIVRKELDQIFIPFYSTKTTGSGIGLSLSKQVMSLHHGNIRVESQTGKGTSFFLEFK
                     SIQGK"
     gene            10418..11782
                     /locus_tag="VGA21_10360"
     CDS             10418..11782
                     /locus_tag="VGA21_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002699423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX9650954.1"
                     /translation="MSLKFDRRKFIKTGTITGIGVSMIGFPNIMMGKDDRKARLGFIG
                     VGLRGRNHLFLALQREDVLVNAICDIDPAAIETSQKMIREAGRKEAAVFKAGEKDFLN
                     LLERDDLDGVIISTPWRWHTPMAVAAMKSGKYAGVEVSAANTLEECWDLVETFESTGV
                     PCMIMENVCYRRDVMAILNMVRLNMFGELVHLECGYQHDLREVKFNDGKNPYGGGIEF
                     GEKGFSEAKWRTWHSVHRNGDLYPTHGIGPVAKYTSINYGNRFVSLTSTASKSRGLHK
                     YIVEKGGEDHPNAKVRFKLGDVITTVIKTANEETIIVSHDTNGPRPYSLGFRVQGTQG
                     IWMDLNKSLYIEGVSEKAHQWEPAQPYLDKYDHPLWKKYADLATGAGHGGMDFFVDHA
                     FIEAVKRKEPTPLDSYDCAAWSAISPLSEQSIALGSMSVDFPDFTRGKWMSRKDRFAI
                     SDEY"
     gene            complement(12047..12346)
                     /locus_tag="VGA21_10365"
     CDS             complement(12047..12346)
                     /locus_tag="VGA21_10365"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEX9650955.1"
                     /translation="MVKIIWTKKAFSQLERAIKYIKEEQGHSYAEIVFNKTLEKTRLL
                     ENNPLIGQVEPLLKHKKSEYRYLIAWSYKIIYRIDQDKIIISRVFHTSRNPRKVK"
     gene            complement(12334..12525)
                     /locus_tag="VGA21_10370"
     CDS             complement(12334..12525)
                     /locus_tag="VGA21_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009583825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650956.1"
                     /translation="MKPEKIKEYLKQIAENVTPETQLEDIYEQLALLSDIDESEEQEQ
                     KGEIFTQAQVEEKSKEWLK"
BASE COUNT         4100 a         2545 c         2521 g         3803 t
ORIGIN      
        1 tcgataaatt ctatcatgct tatcaaattt ctgctcatag cttagctctt gcatagcata
       61 aagcagtatc acaaaactgc acgcgattcc aattgccagt ccgccgatat tgattatgga
      121 ataactcaat tgttttctta ggtgacggaa tgctatttta aaaaatgact cgatcataat
      181 tttttaattt tattcatttc ttaatgcgcc tttattcggt acgtaatgat ttaaccggat
      241 ttgcggttgc tgagcgcatt gattgtaagc taatagccgc gattgttatt aatgccgcta
      301 taataagcgc tagtataaat actagtacgc caatatttac atgatacgga aaggtagtca
      361 gccatttatt taaaatgtaa tagctgaccg gccaggaaat cagattcgca ataagcacga
      421 gcccaagata ttctctatac aacattttta gaatggaagt tatatcggct cccaatactt
      481 ttcttattcc gatttccttt gttttaagtt gagcggtata tgatgatagt ccaaacaacc
      541 caaggcacga tattatgata ctgagcacag aaaatgctcc aaatattttt cccatcaaaa
      601 cctcagaatt ataatttttt tcaatttcat catcaacaaa aaccaactcc atcggcttat
      661 caggattgaa agatttccat tttgattcca gcatagcaat ggcatcttgc cagttggtat
      721 gattcaatct aattgtgagg taatcggaaa aatatggatg cttattaata tatagtacca
      781 gcggatcgat gctattatag agagattcat gatgaaaatc tttcaccaac cctatcacct
      841 ttcctttcct gacttcttcg ccgttaatat atacggtcat ttgaaaatcc tttcctacgg
      901 gattttccca gcctaactta tcagcagcag cttcattgag tatatatccc gctttcaaat
      961 cggtggggat atcctttgaa aaatttctgc cttcaagaat ttcaatattg taagtaggaa
     1021 taaactcctc atccactccc agtgaggcca tactgaattc agttccttcc ggcatttcct
     1081 ccgaaagtat ttgccattca acaaaatagg aaactacgcc cggaatagta gaagacaatg
     1141 ccacattctc caccattgat tctttgagta attcatcagt taatacctgg tacttgactt
     1201 gcgattcgcg atcaaccagt ctgattgcaa taagttgttc ctcattaaat cccagattag
     1261 tggtgcgcat gaaacttatt tgcctttgaa tggtgatggt tgcaatgatc aggattgatg
     1321 cgacaatgaa ttgaaaaaca accaaaaatt tactaaaact tcctaagcct gactttaaat
     1381 tgcctttatt cgtaagggca gaaacaggag aaaatttgga aagataaaaa gcaggatacg
     1441 agccagataa tagaatgacc aaaactatag atgcaattac cgcaacgagg ttaactcctt
     1501 ccaacagaaa tcctattgaa atggttttgc caattagttg attataaaaa ttaaccagga
     1561 tcaattctgc aagaccgaaa gatataacta cagagataag cgttatgatt aaagattcac
     1621 ccaggtgctg ccctactaat tgcaatttag atgcacccaa aacttttctt aatccaacct
     1681 cgtgagatct cttgattgat tggaccgtac ttagattggt gaaattgata caggcaatca
     1741 caagaataaa tccggcaata atcataaaga atttaatata ctcatagttc gagttggctt
     1801 gccattccga ttccaaatct gaaaacaaat gaatgtctga agtattctga ggaatatatt
     1861 cacgcttctc cgtcttaata taatccggaa aatgattttc gcccatagat ttaatctgag
     1921 tctggaggtc attcggattg gttccatctt ttacttcaac ataaataaac atgggcggat
     1981 aatgccaatt gttgtaccag ggttgaattt tactaattga agcaaaggaa gccagtatgt
     2041 ttgtattaaa gtgagaattc tgaggaatgt ctttaatgat cgcggtgata gtaaactcgt
     2101 acaaatcttc ctcatcttca aaataaaggg atcttccaac cacatccgat ttgccataca
     2161 ttttgattgc tcttgattcg gaaagtacta ccgaaaatgg atgattgaga gcagtattga
     2221 gatcaccatg aaccgcgata aaatcaaaaa cgtcgaaaag aagagaatca gcaaatgtga
     2281 ataaagtttc cttttcctta ttaattttat cggggcttat tattccggtg atgggataca
     2341 ttctcaccac cctggttatg gaaggaatgt gactcctcat cagttccgct aaaggtgaga
     2401 atgataccgc ggaatttaat tccctccctt catcaattct cttttcactt aaacggtaaa
     2461 tattcttgtg gtttgaatga aaggtatcat agcttaattc aaactgaaca tgagccacaa
     2521 ttaccaaaac gcacgctatg ccaatgctca ttccgataat attcaagctc gagctggaaa
     2581 tattccggaa gaggtacctt aaggcgatct ttatataatt acttatcatg attattattc
     2641 ggttcgaagt gattcaacag ggttggcttg cgcggccctt agtgatttat accccactgt
     2701 gatgaaagta atgataatta ccaggacaat agttacgatg aacatccagg cttgtatttc
     2761 aattctgtat gcaaactgat ttaaccagtc tccaattaaa atgtgggcaa tgggtgcggc
     2821 aatgacgaat gcaattacta ccagtatgac aagttcatta acaaaaattt tcattacctg
     2881 ggtggtcgaa gcgcctaacg cctttcgaat tccaacttcc ttcgttttat tatttaccat
     2941 aaacgaaatc aagccataca aacccaggca tccgataaaa agggtaacag cgacaaacaa
     3001 aagtaaagta tccgacattt tttcttgtga cctgtaaaaa agggcaatgg cctcatccag
     3061 aaatgtgtaa tcaaatacat actgaggaaa tacctctttc caggcagatt caatttgcga
     3121 aagagtttca cttacatttt ctgaatttat cttaacgccg gctacgtggt atcttgagga
     3181 cgtatttgtg aaaatcaacg gcgcaattgg ttctgaaagt gatgtagcat gaaaatcttc
     3241 cacaacgccg atgattggta gtattctccc ccacatcaaa atattctgac ccaacaaact
     3301 ctccggctcg tccctcacca gcatttttgc cagcgtttcg ttaatcacaa catttcttgc
     3361 cgtgtcggaa ttttctaaaa atcggcccgc acggagtttt aattcatagg tagataaata
     3421 cgaagaatcc accattttaa cgtgaacgct tatattctct tctccgaggg tttgaggaaa
     3481 cttcatataa gttccccaaa cactgccaga tgccggtggc gagaaaccaa aagacacatt
     3541 ttgcacgttg ctattgctca atagtgaatt cctcagcaca cttagtttac ttgcattttg
     3601 ttccggtaat cttgtaacta caattaaatc ttttttgaaa cccataggag cattcttaag
     3661 gtagtttgtc tgggtcatga tgattattgc gcttatgatc aaaagctgag atactaaaaa
     3721 ctgaaaaaca accaatccac gtcgaaaatt aagactacct cctcgccctc cggatattga
     3781 actacgcaat gcgcgtatcg gactaaatcc tgacatcacc attgccgggt aaagacctgc
     3841 cataaaactc attgccagcc atgccgcgaa taaaaacaca atcataccgg gacctgattg
     3901 atcaatgtga aggtctactt ctaatagtgc attaaactcg atcaaaaaca actctgcaag
     3961 tcctaaccca acaattaaag caagaaggca aataagcgcg gtttcaccta aaaaacggat
     4021 tgcaagttgc actctggaac tccctaacac ctttctcaca ccaacttcac gcgacctttt
     4081 aattgcctgg gcggtagcca tattgatgaa atttatgcag gcggtcaaca atagcaaaaa
     4141 ccctacaatc gacattgtaa gaattgatga tttgggaacg ctcttataaa gataattgga
     4201 ggtgcgcgta tcgaagtgaa ggtctgccag cggctgcaaa agatatctta aagtaccgga
     4261 aggacgatca gcatgatatt tctccacaat ttccggtagc ttttgcgaca gagatggagc
     4321 cgtggaattt tctttcagga gaaaataggt ttgataatca ctagcgttac tgttccaatt
     4381 atccatatta gaggaattat cacgccgata cgtttcatag gaaatcatta cttcaaaagg
     4441 aaagtgagta ttattactat aatcttcaag aatagctcca actaccagat cttttgaatt
     4501 attcaggtta attattttgc caatcggtgt tccctcacca aaatacttct ctgccatttt
     4561 cttcgacaaa gcaacatggt caggctgttt taaagtggcc tttaagtctc cttgcagaac
     4621 attgcgacta aagatttcat agaacgccat gtccaaatag gcaattcccg agttttcgtt
     4681 gtacttggaa aatgatccat cagattctgt aatggtaatc aatccgccga catcataaaa
     4741 aacagatgtt atcttttcaa tttcagcaaa gtcttcctgc attcttggcc cgaggggtac
     4801 aggcactcca gacgaatagt gcatattatc attctgcaaa gtctcggtaa ttacccggta
     4861 aattctatcc ggatttttat gaaaggtatc aaatccggtt agatagctaa tgaggagaaa
     4921 aattattaca ctaaacgcca cacccaggct aagccccatg agattcaatg aagtgaaaga
     4981 tttgtccttc attaactttc tccaactgaa ttgaatgaaa ttctttagca tttcttttga
     5041 gtttttaaca ttgcataatt attcatctct taaaacatcc accgggttag cactggccgc
     5101 ggagtaagat ttataaccca cagtgaacac cgcgataaaa attgaaattc caccagcaag
     5161 tataaatacc catacattaa tatctatatg gtagggaaaa tcagccagcc acgcttccat
     5221 agcaaaatat gaaaccggag ttgccaatat gaaagcaatt aatatcagaa caacaaattc
     5281 cctggtgaac aaccccagca agtgtgctat tgaagcaccg aaaacttttc gaattccaac
     5341 ttcttttacc cgctgttgtg ccatgaagga tactaaaccc aacaatccaa gacaaccgat
     5401 ggctatcgcg ataatgctga aggtagagaa taaaatactt tcccgttgct catcctcata
     5461 gtactccttt atgcggtcat ccaggaaatc atactcaaaa atagatctgg gaaaaacctt
     5521 attgtagatt tgttctattt tcactaaagt tccctgcaaa tcattggttg atacttttat
     5581 gccggccatt gcatattgat tacgaattcc tatcaccaca ggagtaattt tctcgtgcat
     5641 cgatgctgaa aagaaatcct ttactactcc cataaccggc gcttgttcac cccacatgtt
     5701 aataaaatta ccaatcacct cctcaggaga agaggcacca atagcattca gaaattcttc
     5761 attaaccaag aaactattag tagtatcacc tggcaataaa tcttcaccgg caattatttc
     5821 aagtccgtag gttttggcga aatcatgatc gacccatttc acctgggctt cttctctaat
     5881 ctcttcatct cctttataat accggaaact tccaccccag atattgccgc tgctggcact
     5941 agtgtttgaa taagataaat ttttgatagc agtctgctcc agtaattcat tcttaaatga
     6001 ttgaagctga gttttttcat agccctggag tttcacttct attatggctt ctttgttaaa
     6061 gcccattggc gtgttattga agtaattcat ttgcattccc acgataatag tacacacgat
     6121 cagcacttgt gagattacaa attgcaaaat taccaacact cttcttaaac ccagactgga
     6181 tttggatgag cttgcaagct ttttcttgag agcctgcgca ggttgaaatc ctgacaggac
     6241 cattgccgga taccaccccg ataaaattgt tatggtaaca aaaagcacca gaataaatat
     6301 caaagtaccg ggttgcaacc atgagccaaa ctccagggaa tagcccaaca atttatcaat
     6361 atggatcaag gataattcta ctaaaccaag agaaagaaat acacttgcaa gggtaataag
     6421 agaagtttca gagaagaact gaaggattag tccggacctt ctgccaccca ttacctttct
     6481 aataccaatt tcttttgccc gctttaccac ttgaacggtg ttgagattga taaaattaat
     6541 ggaggccgta agtatcagaa aaactccaat caatcccagg gcataaattg atctttcttc
     6601 tacaatacgg gtgaaagtaa agccaatatt tgtatcaaaa tgaacctcgc ttagtttctg
     6661 cagatacaac cgggttcgtt cttcattcgc cccttttgct ttgtatttaa aaaggaaatc
     6721 tgtcaacctg tattccacat cttcgggatc tgcatcctcg tggagcgtaa gaaaacactg
     6781 cgttgcactg cttgtagaat tccaattgtt gttaggttca tcaaatttgg cagcataggg
     6841 tagaaaaatc gtgaaatcga aacttgaatt ctcaggaaaa tcttcagcaa cacctgtgat
     6901 ggttaattca tcttcggaat cttgtaaaat agtctcacct acaacgttcg tcctgccaaa
     6961 atatttattt gctacacttc tggaaattac cacggaattc ttattcggat gggctgcttc
     7021 cggcgagcct tcaatccatt tcaacctgaa catcttaaaa aagtcaggat ccacatagac
     7081 aggggcttca aagaatttag aaatgccgga gttgttatca acgctgaaaa caggatttct
     7141 gaaacttcca tcaataattg ttacatattt ggcttcagga aaatcatctc tgaaggcaga
     7201 tgtaaatggg ttaggcactc cggtgtcaga acctaattcg ccataagaaa cttgttcagt
     7261 aattactctg taaatattct cagcgtcctg atcttcatca tagctcagtt caaagcggat
     7321 aattgaaaaa atcagaattg cgcaggtaat accgatggac aatccaaaaa catttatagc
     7381 actgtaaaaa ctatttctca ggaaatttct aactgcggtt ttaaagaaat tctggatcat
     7441 ctggactggt tttagagttt cttagccaaa ataaatgcca ataagcaagt aattgacaat
     7501 cattgatttg caggtattga ttaatattat tcaagaaact atgtatcatt ttcaatacac
     7561 tttgccgtat gtttatgata cactacattt taatacaaat tggaaagaca ggaaggctca
     7621 ttattaattg ttgatgacga ccgcgacgtg cttgaaactg ccaggatgtt cctaaagcag
     7681 cagtttgaac aagttattat tgaaccaaat cccaatgcaa ttccgcggca catcgaagaa
     7741 tttaatccgg atgtgatttt actggatatg aatttcagga aagggatgaa tgatggtgaa
     7801 gagggatttt attggttaaa gcaaatattg gataaggatg aaaatgcggt cgtaattctc
     7861 atcaccgcgt acggtgaagt cgacctggcc gtaaaagcta tcaagatggg agctattgat
     7921 tttgtattga agccctggaa gaatcaaaag ttgttgggaa caatactatc cggtcttaaa
     7981 ttacgaaaat caaagctgga ggttggaagg ttgcgcacaa cccagcgaaa actggaggat
     8041 aatatttcca gacaattcgg aaattttata ggtgagtctc ctgtgatgca aagagtgttc
     8101 gaattaatta acaaagtcgc ggatacggat gccaacgttt taatactagg tgaaaatgga
     8161 accggaaaag aattggttgc aagggcgctg cacaacaagt catccaggaa gaataatgga
     8221 tttatttccg tcgaccttgg cgcggttcct gaaaatctat ttgaatccga actattcgga
     8281 cacacaaagg gtgcttttac cgatgcaaag gataatcgag taggtagttt tgaacttgca
     8341 tcaggtggaa cattattcct ggatgaaata ggtaatatta cacctccact gcaggcaaaa
     8401 ctacttgctg ttattcaaaa tagaaaaata aagcgggtag gttccaataa agatataaat
     8461 atcgatatca ggttaatatg tgcaactaac ttgcctctat atgaaaaggt acgtgatgga
     8521 agttttcggc aggatttgat gtatagaatc aataccgtag aaattatgct tcctgccctt
     8581 cgggaaaggt cagaggatat tgctttgtta acccggcatt ttgcagacct ttattgcaag
     8641 aaatatatga aaccaacaat gaaggttagt aaggaaacca taagcagact gcaaaactac
     8701 aactggccag ggaatgttcg tgaattacag catgcaattg aacgcgcggt tattctggct
     8761 gaaggcaact cattgcaatc ttctgatttg cttttaagag agcctacatt ggaatcaaaa
     8821 ccttttttta aagcaaccac attggaagaa atggaaaaga aatttatcct tcaatcacta
     8881 aaagataacc agggtaatgt aactcaaaca gcaagaaccc tgggtctcac gcgcacagca
     8941 ttatatagga ggctaaataa atatggctta taaatggttt tgaaaagatt ttcactcctt
     9001 gtaattctga ggataattct gatccttggc aatgttatag taattgcatt tatcttgggc
     9061 gatgccaggt tattctttaa tcagattatc cttttcattt ttctcaccgc acaaattgca
     9121 gacttaattc gttacgtcaa caaaaccaac cgggagttgg taaaattcct taatgcaata
     9181 aagcaatcgg atttttccat gactttctct cagaattcag ccggcaaatc ttttagtgaa
     9241 ttattccagg cttttgagac tatcattaat tcatataaga aagttaaaat tgaaagggaa
     9301 gcccagtatt tgttcttgca aaaactcgtt gatcatattc atgttggaat aattgccgtt
     9361 gaaaacaaag acaaacttgt tctgcttaac aattcggcgc gaaagttatt ggagattcct
     9421 gaacttcgaa actggaaagg cttggaggcc agaactccag atttaatcag ggaagtaaaa
     9481 ttactcgaac ctgatggtcg caaattggcc gggatcgcgg tgggtaataa taagaaaaat
     9541 ctctctttgg atgtttcttc gattcaactt attggtgagg acacaatttt gattactttt
     9601 caagatataa aaagcgaaat tgaacaaacg gagatagaag catggcataa acttatacgt
     9661 attctcaccc atgaaattat gaattctgta acacccatct catcattaac agaaacgatg
     9721 caaatggtac tgcgaaaaga ggataggatt aggaaagcag aagaaatcaa tgacgaaacc
     9781 attgaagatt tggccttttc actcgagacg attcaaaaaa gatctgattc tttactttct
     9841 tttgtagata gttacaggaa gttaacaaag gttccgaggc caatacttgg acaggttaat
     9901 tcaaaggaat tactggaagc ttcggtcaaa ctgctgcaag cagagattaa caaaaataat
     9961 atagaggtta gcattgaatt tgatgatccc ccaattgaaa tttacctcga tcgaagcctg
    10021 gttgaacaag ttctgattaa tttaattacc aatgcaatac acgcattgga aggaatagcg
    10081 aatcctaaat tgattcttcg ctcgtatgca aatgaaaacc gcaaagtcat tcaaatcaca
    10141 gacaatggag ttggcattgt gaggaaagaa ttggatcaga ttttcatccc cttttattca
    10201 acaaaaacca caggaagcgg gattggcctt agcctcagta aacaggttat gagtcttcat
    10261 cacggaaata tccgggtgga atctcaaact ggaaaaggca cctctttttt tcttgaattt
    10321 aaatctatac aaggaaaata atcaacaaaa taatttacat atttctttag ctctcctgta
    10381 tatgataact atttttaact gaaaaaataa tcacgccatg agtttaaaat tcgacagaag
    10441 gaaatttatt aagaccggaa cgatcacagg aattggagta tcaatgatcg ggtttcccaa
    10501 tatcatgatg ggaaaggacg accgcaaggc aagactggga tttataggtg ttgggcttag
    10561 gggaaggaac catttgttct tggcattgca aagagaagat gttttggtaa atgccatctg
    10621 cgacatcgat ccggcagcaa tcgaaacttc acaaaaaatg atccgtgagg cgggcagaaa
    10681 ggaagctgca gtttttaaag ctggagaaaa agattttctc aacctcctgg aaagggatga
    10741 tctggacgga gtaatcattt caacgccctg gcgatggcat actcccatgg ccgtcgcagc
    10801 catgaagtct ggaaaatacg cgggtgtaga agtttctgcc gctaacactt tggaagagtg
    10861 ctgggacctg gtggaaacct ttgaatctac cggagttcct tgcatgatta tggaaaatgt
    10921 ttgctaccgc agggatgtga tggccattct aaatatggtg cggctaaata tgtttggtga
    10981 attggtacac ctggagtgcg gatatcagca tgatttgcgg gaggtgaaat ttaatgatgg
    11041 caagaatccg tacgggggcg gcattgagtt tggtgagaaa ggattttcgg aagctaaatg
    11101 gcgtacctgg cattccgtac atagaaacgg cgatctctac cctacgcatg gaattggtcc
    11161 ggtggcaaaa tacaccagca ttaattatgg gaaccgcttt gtatcgctga cttccaccgc
    11221 atccaaatca agaggcttac ataaatatat tgtggagaaa ggtggcgaag accatcctaa
    11281 cgcaaaagta agattcaagt tgggagatgt aattaccacg gtaattaaaa ctgccaatga
    11341 ggaaactatt attgtatcac atgacactaa tggccctcgt ccctactccc tgggattcag
    11401 agttcaggga actcaaggca tttggatgga tctgaacaag tcgttgtata tagaaggtgt
    11461 gagtgaaaag gctcatcagt gggagcctgc tcagccttat ctggataaat atgaccatcc
    11521 tttatggaag aagtacgctg atctggcaac tggtgccggt catggcggca tggatttttt
    11581 tgttgatcat gcatttattg aagcagtgaa acgtaaagaa ccaactccgc ttgactcgta
    11641 tgattgcgcc gcctggagcg ctatcagtcc attgtcagag cagtctattg ctttaggaag
    11701 catgagtgtt gactttcctg attttacaag gggtaaatgg atgagtagga aagataggtt
    11761 tgctatcagt gatgagtact gaacagtgat tcagtgaaca atgaatggtg aacagttata
    11821 aatataagtc caaaaccatg tggtagtcaa accccctatt cccccttaaa acaaggggga
    11881 caatttctag gcagagggta ctttcccctc cttaatttaa ggttgggcct gttgaccgaa
    11941 ggttgggtta ggggtggttt tacaccaaaa cttaccttac ctgtgggcgc cattggaagg
    12001 cattgtttgt agccccccta ttaagggcag ctggcttagt ctatattcac ttcacctttc
    12061 gagggtttct tgaagtatgg aaaactcttg agattatgat tttatcttga tcaattctgt
    12121 agattatttt ataactccag gcaataagat atctgtattc tgattttttg tgtttcaata
    12181 gtggttcaac ttgaccaatt aatggattat tttcaagaag gcgggttttt tcaagagttt
    12241 tattaaatac tatctcagca taagaatggc cttgttcttc tttaatatac ttaattgctc
    12301 tttcaagttg ggagaatgct tttttagtcc aaattatttt aaccattctt tagatttctc
    12361 ttcaacctga gcttgagtga aaatttcgcc tttttgttct tgttcttctg attcgtcaat
    12421 gtcagaaaga agcgccagct gctcatatat atcctccaat tgggtttcag gagtcacatt
    12481 ttcggcgatt tgcttaaggt attcttttat tttttcaggt ttcattaaaa tgcatgtaaa
    12541 tcaaatatac cgaataattt attaataacc gaaaaaggac aattgccccc tgctcggatt
    12601 aatcgaaaat ccactctatg tttaatcccc aatttatttt aaggttggcc tgccatccga
    12661 gggttggtta ggggtggtta tgacaggtag tcaaaccccc tattcccact taaaacaagg
    12721 ggacaatttc ctgggcaggg tgtactttcc cctccttaat ttaaggaggg ggcaggggtg
    12781 gttataaaaa tagaaatgca gctgttatta tgtgggaagg aaataacccc ctgtagtccc
    12841 acttaaaaca aggggacaat ttcctgggga atttacactt tagaaaattt cagatcattc
    12901 gtaacgaagc acaattgccg gatttaagcc agccacccta aaagctttgt agctcactgt
    12961 aattaaaga
//
LOCUS       DASWHX010000155         8336 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_152415, whole genome shotgun sequence.
ACCESSION   DASWHX010000155 DASWHX010000000
VERSION     DASWHX010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 8336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8336
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152415"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..280
                     /locus_tag="VGA21_10375"
     CDS             <1..280
                     /locus_tag="VGA21_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008198856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX9650957.1"
                     /translation="GKKVYISGDTEDIPEMRALRNIDIAFVCMNQPFTMEVEQAADAV
                     LEFKPDIVYPYHYRGREGLSDVKKFESLVKEGDSNIEVRLREWYKPEN"
     gene            complement(346..783)
                     /locus_tag="VGA21_10380"
     CDS             complement(346..783)
                     /locus_tag="VGA21_10380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650958.1"
                     /translation="MKISKLLTIFGKGKTVSKSHIRNLLEMARADGKVDDLENELLFM
                     LAERYEVSPAIINRMSANEYEVVLDLPDNEREKFDQFFDLVQMMMADNFMHEDEINLC
                     YFFAVRYGYKKDRIENLVQEAVVTIREGGDSESAWDKVKLLLG"
     gene            complement(819..1256)
                     /locus_tag="VGA21_10385"
     CDS             complement(819..1256)
                     /locus_tag="VGA21_10385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650959.1"
                     /translation="MKILSFFTRNGKNKEVSEKHLKNLFELAHVDGHFDSREEKLLEI
                     IAKREKIGSDIIKKFASETNGQEMKIPDEEELKFNQFFDLVKMMLADEYIHVLEMDLI
                     RSWGERFGYNKKHLDELIDSIAENIKVGHDANDTIQRVRWMFK"
     gene            1539..1871
                     /locus_tag="VGA21_10390"
     CDS             1539..1871
                     /locus_tag="VGA21_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020599293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEX9650960.1"
                     /translation="MKGTQLGEFEELVLLTVAALNNQAYGISIKDEIENRGKRKISIG
                     SLHSTITRLQEKGYLESKLGSATPERGGRSKRFFFLTHEGKVALHRCKDLRDQLWNLS
                     PETLSLKK"
     gene            1868..4501
                     /locus_tag="VGA21_10395"
     CDS             1868..4501
                     /locus_tag="VGA21_10395"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9650961.1"
                     /translation="MKINSNKINPPRGAIRFIEWQLGNSAEDLIGDLEELFARDITNR
                     KVWIAQILFWYRAVYFSISKTGRVRKKFNFGKNRYSNTSVNLFGSYLKVAVRNVLRSK
                     LFSIINVAGLALGMSAGLLVISMTNDLLKFDEFHENKDYLHRVVTNAAYYNYGWSERA
                     TTCEPVASVLKQTGGVKNVVRIQKRLSGEVDSGAKILPLSGYFADEDFFEIFSFDFIR
                     GGAENSLTRPFQIVLTENTAIKLFNNLNVMDSTLKVGNLGYFTITGIVKDVPKASHMQ
                     FEMVASFSTVPILERDKILSAKESDWTDVNGTYVYCLLEPDASIETIENTLNREASEI
                     YANFDNVKAAFSLQNLKSIVPGKDLSNSLGPKMIDIAIYIMSGLAFMILLSATFNYAH
                     LSSARLLQKSREIGLRKVIGGTRKQLFIQFISETLVVSFISLILSIGIFILIRPHFLA
                     IVPRASEMLDLEITSELFLYFLLFALLAGILAGTLPALYFSRLNPVQALRKVNRAGKK
                     FTSKKSLVVAQFTLSIIFILGVFIISKQYTFSLQYGLGFKPDSTIVMPIYGINPEIVK
                     GEIQKLNEVESIAMGSLIPGTGSTQFSWIKNSEAADSLSSFYMSVDEKFVPALDVKLL
                     AGRNFHPEMLEPHSILINEHLARMLGFDNYQNALEKLVRIQGKEYRIIGVVSNFHYTH
                     LEEPIKNFYLVSNHEQFYYAFVKVNSANITQTFGALENAWLKFAPKKDFNARFMEEHL
                     QRAYSHYVNIMKIFGFLGTLAITIACLGLLGIAVYSMQLRVKEVGIRKVMGATELGLV
                     GLLSRNFLAMMAIAICIGVPIAYFFFNLVLLPLNYYHTKVGVLEIFYSIVLMTGLGLL
                     MIGSQTLKAATSNPAEVLRNE"
     gene            4546..5853
                     /gene="der"
                     /locus_tag="VGA21_10400"
     CDS             4546..5853
                     /gene="der"
                     /locus_tag="VGA21_10400"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010608982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEX9650962.1"
                     /translation="MANIVAIVGRPNVGKSTFFNRLVENRQAIMHDESGVTRDRHYGY
                     AEWTNKYFTVIDTGGYVTGSDDIFERKIRHQVEIALEEASCIIFLVDCSVGLTDLDKD
                     FAKVLRKSKKPVLIAANKADNPERMLVANEFYRMGFELIFPISSVSGSGTGELLDELV
                     SNFEHTDVANPEAGIPKISVLGRPNVGKSSFVNILLGQERALVTEIAGTTRDSLNSHY
                     KLFGKDFILIDTAGIRKKSKVQENIEFYSVMRAIKALQDSDVCIILVDASLGLESQDL
                     SIINLADKYRKGILIMVNKWDLIEKDHKTAETFKKTINEKLGNLSYIPVIFTSVLKKQ
                     RIFQVIELAVKIQENRSKRVPTSQLNDVLLPIIERNPPPSTKGKHIRIKYITQLPAKT
                     PAFGFFCNLPQYIKPPYKRFLENQLRSRFEFEGVPVSLFFKKK"
     gene            5920..6120
                     /locus_tag="VGA21_10405"
     CDS             5920..6120
                     /locus_tag="VGA21_10405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650963.1"
                     /translation="MKKLFLMFLITGFIVAFTSCGGGSSTEAETEAETEEPAATEEEG
                     TESEATEEPDSTATEEADSTAT"
     gene            6220..6846
                     /locus_tag="VGA21_10410"
     CDS             6220..6846
                     /locus_tag="VGA21_10410"
                     /inference="COORDINATES: protein motif:HMM:NF021759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT-like domain-containing protein"
                     /protein_id="HEX9650964.1"
                     /translation="MKGKDLMRVLLMKHRYPEWVVRYCVRLKRHYPFEFVQENSRFSK
                     LGDFTYRTRTQPYVITLNKDLNKDQFLITLVHEIAHRVTAEKFGRRVQPHGNEWKRQF
                     QRLMKPLLKNEVFRPSVLKPLEKHLENPKSTTFADPILIKALNSRMQKGHFFLSDIGI
                     GRAFSFNGRYFKKLRTQRTNVLCREIGTRSNYLVPATAIVQRVRARLR"
     gene            complement(6857..7675)
                     /gene="murQ"
                     /locus_tag="VGA21_10415"
     CDS             complement(6857..7675)
                     /gene="murQ"
                     /locus_tag="VGA21_10415"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009583704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HEX9650965.1"
                     /translation="MSITESSSKYDGLENMSVRDLLININLEDKTVPKSVERCLPQIE
                     ALVVEVTRQMKKGGRLFYIGAGTSGRLGIVDASECPPTFGVEHGLVVGIMAGGDKAIR
                     RAVEFAEDDSERAWKDLQEYQINHKDVVVGLAASGRTPYVIGGVKKARDHGIITGCIT
                     CDRNTELAKSVIYPIEVIVGPEFITGSTRMKAGTAQKLVLNMISTSVMIQLGRVKGNK
                     MVDMQIANNKLIDRGTRMIMEELSVGRFEAEELLKKHGSVRKVIEAIRKSGGEI"
     gene            complement(7697..>8336)
                     /locus_tag="VGA21_10420"
     CDS             complement(7697..>8336)
                     /locus_tag="VGA21_10420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HEX9650966.1"
                     /translation="KISESIQKYFANARISVNHDLLAVARALCSNERGIACILGTGAN
                     SCLYDGKEIVENIPSLGYILGDEGGGAHLGKSLMAHYFNKTLPADLSVQLERRYDMSR
                     NTVLENVYQKKKPAQYLAGFSKFLFDNKAHPFVDSLIRESFKLFFERNVLKYKDAASV
                     PIHFSGAIAFYFNSIIRQIANDYGLTIKNVVEGPIAGLALYHGGKMEDKLNS"
BASE COUNT         2470 a         1698 c         1628 g         2540 t
ORIGIN      
        1 aggcaagaaa gtttatattt caggagacac ggaagacatt cctgaaatgc gggcattgag
       61 aaatattgat atagctttcg tgtgcatgaa ccaacctttt actatggaag ttgaacaagc
      121 agccgatgcg gtgctggaat ttaaaccgga tattgtttat ccctaccact accgcggtcg
      181 ggaaggcttg agtgatgtga aaaaattcga atctttagtg aaagaaggcg attcaaatat
      241 agaggttcgg ctaagggagt ggtataaacc agaaaattaa tttctgctgt catttgattc
      301 tttaccagat tgataccttt agacggtgtt cgacaaaacc ggattttatc ccagtaaaag
      361 tttcacttta tcccatgcac tttctgaatc tccaccttcc cggatagtta ccacagcttc
      421 ctgaaccaga ttttcaatcc ggtccttctt atatccatac ctcacagcaa agaagtagca
      481 taaattaatt tcatcttcgt gcataaaatt atcagccatc atcatttgta caaggtcaaa
      541 aaactggtcg aacttttccc tttcattatc cggaagatcc aacacaacct catattcatt
      601 agcactcatt ctattaatta tcgccggact tacttcgtat cgctcagcaa gcatgaataa
      661 tagctcattt tccaaatcat caaccttacc atctgcccgg gccatttcca gcaagtttct
      721 aatatgactt ttggaaaccg tttttccctt tccaaaaatt gtcagaagtt ttgaaatttt
      781 catagtcgta attatctgct aaaatcaatt atggggttct atttgaacat ccatcttact
      841 ctctgtatcg tgtcgttggc atcatgccct actttaatgt tttccgctat ggaatcaatt
      901 aattcatcca ggtgtttttt gttgtagcca aatcgctcac cccagcttcg aatcaaatcc
      961 atctccaaaa catggatgta ttcatccgcg agcatcattt ttaccaggtc gaagaattga
     1021 ttgaatttta attcctcttc atcaggtatc ttcatttcct gaccgttggt ttcagaagca
     1081 aatttcttaa ttatgtcgct tccaattttt tctctttttg ctatgatttc aagcaatttc
     1141 tcttcccgtg aatcaaaatg accatcaaca tgagccaact caaaaagatt tttaaggtgt
     1201 ttctcagata cttctttgtt tttcccgttt cgggtaaaga atgatagaat tttcataatg
     1261 cctttatttt tttgtttaca caattcaagg cagtagctca gataattctt caacttatta
     1321 actccatcaa tttctcagga gcgcagcagg acttactaaa ttataatatt attaatattt
     1381 attatacttt tttcaattaa taattaacat aatagtcatt agttcataat tatcttgtga
     1441 tattatatga ggttggacag ggatggatat atttggttgc aacctctaaa tgccttcaac
     1501 cgtaaaagtt atatatttgt tctgaataat agaaatatat gaaaggaacg caacttggag
     1561 aatttgagga gttggtcttg cttacggtag ccgctttaaa taatcaggcg tacggtatca
     1621 gtattaagga tgaaatcgaa aatcggggta agagaaagat cagtattggt tcgctccact
     1681 ctactattac ccgactccag gaaaaaggat atcttgaatc aaaactcggc agtgctacgc
     1741 cagagcgagg cgggagaagc aagcgatttt tctttttaac ccatgaggga aaagttgcgt
     1801 tgcacaggtg caaggatttg agagatcaat tatggaacct ttcacccgaa actctttcac
     1861 ttaaaaaatg aaaattaatt caaacaaaat aaacccaccg cgaggtgcaa ttagatttat
     1921 cgaatggcaa ttgggtaatt ctgctgaaga tttaatcgga gatctggaag aactctttgc
     1981 aagagatatc acgaatagga aagtttggat agcacagata ttattttggt atcgtgcagt
     2041 ttatttttct atctccaaaa ccggaagagt tagaaagaaa tttaattttg gcaaaaaccg
     2101 ctattcaaat acatcggtta atctttttgg cagttacctg aaagtggcgg ttagaaatgt
     2161 acttagaagc aaactttttt ccattattaa tgtcgccggt cttgccctgg gaatgtctgc
     2221 cggattgttg gtaattagta tgactaacga ccttttgaag ttcgatgaat ttcatgaaaa
     2281 taaagattat ctgcataggg ttgtaacaaa tgcagcatat tataattacg gctggtctga
     2341 aagagccact acctgcgagc ctgtggcctc tgtactaaag cagaccggtg gagtaaaaaa
     2401 tgtagtccgt attcaaaagc gattaagcgg agaggtggac tcaggtgcta aaatactccc
     2461 cttatccgga tactttgctg atgaagattt ctttgaaatt ttctccttcg actttatcag
     2521 aggtggagcg gaaaattctc ttaccaggcc ttttcaaatt gttcttaccg agaatactgc
     2581 cataaaatta tttaataatc ttaatgtaat ggacagtacc ttaaaagttg gcaaccttgg
     2641 ctattttact attaccggaa tagtaaaaga tgtacccaag gcatctcata tgcaatttga
     2701 aatggtggca tccttttcca ctgtgccaat cttagaaaga gacaaaattt tgtcagccaa
     2761 agaaagtgac tggaccgatg tgaatgggac ctacgtatat tgtttgcttg agcctgatgc
     2821 ttcgatagaa accattgaaa ataccttaaa tagagaagca agtgaaattt acgcaaactt
     2881 cgataatgtg aaagctgctt tttcacttca aaatttaaaa agcatagttc cgggtaaaga
     2941 tcttagcaac tcccttggtc ccaaaatgat tgacatcgcc atttacatta tgagcggcct
     3001 cgcctttatg atcctgttgt cagcaacttt taactatgca cacttatctt cggcaaggct
     3061 gctacaaaaa tccagagaaa ttggtttaag aaaagttatt ggtggcacca gaaaacaatt
     3121 gtttattcaa ttcatcagtg aaacactggt cgtgtccttc atctctctaa ttttatcaat
     3181 tggaattttc attctcattc gccctcattt tctggccatc gtgccgagag caagtgagat
     3241 gcttgacttg gaaattacct ctgaactgtt tctttacttc ctgctgttcg cactgttagc
     3301 cggcatcctt gccggcacac ttcctgccct gtatttttcc agattgaatc ccgttcaggc
     3361 cctccgcaag gttaaccggg cggggaaaaa gtttacatca aaaaaaagcc ttgtggttgc
     3421 tcaatttaca ttatctataa tcttcattct tggcgtattc attatttcaa aacaatacac
     3481 gttctcactt caatacggat tgggatttaa acccgatagt accattgtta tgcccatcta
     3541 tggaattaat ccagaaattg taaaaggtga aattcagaaa ttaaatgaag tggaaagtat
     3601 cgcaatgggt tctttgatac ctggaaccgg ctccacacaa ttcagttgga taaaaaattc
     3661 agaagctgcc gattctttat ccagttttta tatgtccgtg gatgaaaaat ttgttccggc
     3721 attggacgtg aaattactgg ccggaagaaa ttttcaccct gaaatgcttg agccacattc
     3781 aattttgatt aacgagcatc ttgcaagaat gcttgggttc gacaattatc aaaacgcatt
     3841 ggaaaaactg gtgcgaattc agggaaaaga atatcgaatt attggggtag tcagtaattt
     3901 tcattacact catcttgaag agcctatcaa gaatttttac ctggtatcaa atcatgaaca
     3961 gttttattat gcatttgtaa aagttaattc agccaacatc actcaaacct ttggcgcact
     4021 ggaaaatgcc tggctaaaat ttgctcctaa aaaagatttt aatgctcgat tcatggaaga
     4081 gcatttgcaa agagcgtata gtcattacgt aaacattatg aagatttttg gtttcctggg
     4141 aaccctggcg ataaccattg cctgcctggg attgctgggc attgcggttt attctatgca
     4201 acttagagtg aaagaggtgg gcattcgaaa agtaatggga gctacggagt tgggtttggt
     4261 gggacttctc tcccggaatt ttctcgcaat gatggcaatt gctatttgca taggagttcc
     4321 gatagcttat tttttcttca atctggtttt gcttccattg aattattacc acacgaaagt
     4381 tggtgtgctg gagattttct acagcattgt tttgatgacc ggtctcggac ttcttatgat
     4441 aggctcgcag actttgaaag ctgctaccag caaccccgct gaagtcctga gaaacgaata
     4501 attcctgctc tcgcattgac ttggaaatga cctaactttg cgtccatggc aaatattgtg
     4561 gctattgtgg ggcgtccaaa tgtcggaaag tcaactttct tcaaccggtt ggtagaaaac
     4621 aggcaggcca ttatgcacga tgaatctggt gtaacacggg accgtcatta tggatatgca
     4681 gagtggacca ataaatattt taccgtaatt gatacaggag ggtatgtaac aggctctgat
     4741 gatatttttg aaagaaagat ccggcatcag gtagaaattg cccttgaaga ggccagttgt
     4801 ataatttttt tggtggattg ttcagtggga cttactgatc tggataaaga ttttgcaaaa
     4861 gtcctgcgca aatccaaaaa gccggttctg atagcagcga acaaggcaga taacccggaa
     4921 cggatgctgg tggctaatga gttttataga atgggatttg aattgatatt cccgatttca
     4981 tctgtaagtg gcagcggtac cggggaactg ctggatgagt tagtgtcaaa ctttgaacat
     5041 accgatgtcg ccaatcccga ggctggcatt cccaaaataa gtgttctggg cagacccaat
     5101 gttggaaaat catcattcgt aaacattctc ctcgggcagg aacgagcctt ggtaacagaa
     5161 attgcgggaa ccacaagaga tagcctgaat agtcattaca aattatttgg taaggatttc
     5221 atacttatag atacagcagg gataagaaag aaatcaaagg ttcaggagaa tattgaattt
     5281 tacagtgtta tgcgtgcaat aaaggcgttg caggattctg acgtttgtat catccttgta
     5341 gatgctagtc ttgggctcga atctcaggat ttaagtatta ttaaccttgc tgacaaatac
     5401 agaaaaggaa ttctgataat ggtgaataag tgggatttga ttgaaaaaga ccataaaact
     5461 gctgaaacct tcaagaaaac gatcaatgag aaattgggta acctcagcta tatccctgta
     5521 atctttactt ccgtattgaa aaaacagcga attttccagg ttattgaact agcggtgaaa
     5581 attcaggaaa accgttcaaa aagggtcccg accagtcagt taaatgatgt gctgctacca
     5641 ataattgaaa gaaatccgcc tccttccact aaagggaagc atatccggat taaatacatt
     5701 acccaattgc cagctaaaac tcccgccttt ggctttttct gcaatttgcc ccaatatata
     5761 aaacctccct ataagagatt cctggaaaat caactccgat ccaggtttga attcgagggt
     5821 gttcctgtca gtttgttttt caaaaaaaaa taggctgata tttatcaggt agtcaaaaac
     5881 tttctaactt tgaccccgca accaaaaact aaattgaaga tgaaaaaatt atttttgatg
     5941 tttttaatta ctggcttcat tgtcgcattt acttcatgcg gtggaggatc cagcacggaa
     6001 gctgaaactg aagctgaaac tgaggaaccg gcagcaaccg aagaggaagg tactgaatct
     6061 gaggctacgg aggaacctga ttcaactgca actgaggaag ccgattcaac tgcaacttag
     6121 aacacaggtg aaggaagcga atctgaagat tcttcaggaa gctaatttcc aacgaataac
     6181 actgtaatcg aggctttagt ttatctaaag ccttttttta tgaaaggaaa ggatttaatg
     6241 agggtattgc ttatgaaaca ccgctatccg gagtgggttg tgcggtattg cgttcgcctg
     6301 aagcgacatt atccctttga atttgtgcag gaaaattcca gatttagcaa gttgggtgat
     6361 tttacttacc gtactcgtac ccaaccctat gtgattaccc tcaataaaga cctgaacaaa
     6421 gaccagttcc tgataacctt ggttcatgaa attgcgcacc gggttactgc agagaaattt
     6481 ggccgccggg tgcaacccca tggaaatgaa tggaaacggc aattccaaag gttgatgaaa
     6541 ccgttgttga aaaatgaggt gttccgaccc tcggtcctta aacccctgga aaaacacctt
     6601 gaaaatccaa aatctacaac ctttgctgat cctatcctca tcaaagcctt gaacagcagg
     6661 atgcaaaagg gacatttctt tctgtccgat atcggaatcg gtcgtgcatt tagttttaat
     6721 ggcaggtatt ttaagaagct gagaactcag cgcactaatg tattatgccg tgaaattgga
     6781 accagatcta attatcttgt tccggccacg gcaatcgttc aaagagttag agcccggctg
     6841 aggtagagta tcagaatcaa atttccccgc cgctcttgcg aatggcctca attactttcc
     6901 ggacagaacc atgctttttc agtagttctt cagcttcaaa tctgcctacg ctaagttctt
     6961 ccataatcat gcgtgtaccc ctgtcaatca atttattatt ggctatttgc atatcaacca
     7021 tcttatttcc tttaaccctt cctaattgga tcatcacact ggtgcttatc atattcagca
     7081 caagcttttg ggcagttcct gctttcatcc tggttgaacc tgtaatgaac tcagggccca
     7141 cgatcacttc tattgggtaa ataacggact tcgctaattc tgtatttctg tcacaagtaa
     7201 tgcaacctgt tataattcca tggtctcttg ctttcttcac accaccaatt acatagggag
     7261 ttcttcctga ggcggctaat cctaccacta catccttatg attaatctga tattcctgca
     7321 aatccttcca ggcccgctct gagtcatcct cagcaaactc gactgcccga cgtatcgcct
     7381 tatccccgcc ggccattatt ccaactacca acccgtgctc tactccaaaa gttggggggc
     7441 attccgatgc atctacaatt cccaggcgcc cgctggttcc cgctccgata taaaataatc
     7501 tgcccccttt cttcatttga cgagttactt ctactaccag cgcttcaatt tgcggaagac
     7561 accgttcaac tgacttggga acggttttgt cttcaaggtt gatgtttatc aggagatccc
     7621 tgacagacat gttttctaac ccgtcatatt ttgaacttga ttcggttata ctcacaacac
     7681 taaaatttat tcaaagttaa gaattaagct tgtcttccat cttcccgccg tgatataaag
     7741 ccaacccggc tataggacct tccaccacat ttttaatggt caaaccataa tcatttgcaa
     7801 tttgtcgaat aatactgttg aagtagaatg caatagcacc gctaaaatga attggtacgc
     7861 ttgcagcatc cttatatttt aaaacgtttc tctcaaaaaa gagtttaaat gattcccgta
     7921 tcaggctgtc tacaaaagga tgcgctttgt tatcaaaaag aaatttggag aatcccgcca
     7981 ggtattgcgc tggttttttc ttttgataaa cgttttcaag cactgtattt ctactcatat
     8041 catatcttcg ctccaattgc acggataaat cagcgggtaa cgttttattg aaataatggg
     8101 ccattaaact tttccccaaa tgcgctccgc caccctcatc gccaagtata tatcctaaag
     8161 aaggaatatt ttcaacaatt tcttttccat catacaaaca agagtttgcc ccggtaccca
     8221 ggatgcaggc aattcctctt tcattactac atagtgctct ggcaacagca agaagatcat
     8281 gatttaccga aatccttgca ttggcaaagt atttctgaat cgattctgaa attttg
//
LOCUS       DASWHX010000156         2748 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_1263256, whole genome shotgun sequence.
ACCESSION   DASWHX010000156 DASWHX010000000
VERSION     DASWHX010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 2748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2748
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1263256"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..392)
                     /locus_tag="VGA21_10425"
     CDS             complement(<1..392)
                     /locus_tag="VGA21_10425"
                     /inference="COORDINATES: protein motif:HMM:NF042521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6577 family protein"
                     /protein_id="HEX9650967.1"
                     /translation="MNKKLEDTIRKYLSKKGQISKQKLAKAISRDFPSWSEATIDIRI
                     SQLKKQGIIRNPSKGIYSLGTKTSYKPEPSRNLKKLYNRLRKEFPFATFCVWDTNWIS
                     EFMRHQSFKHYLVLEAEKEVMESAFNLLT"
     gene            complement(726..799)
                     /locus_tag="VGA21_10430"
     tRNA            complement(726..799)
                     /locus_tag="VGA21_10430"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(763..765),aa:Ile,seq:cat)
     gene            875..1504
                     /locus_tag="VGA21_10435"
     CDS             875..1504
                     /locus_tag="VGA21_10435"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HEX9650968.1"
                     /translation="MDAVTPEAIIFDLGGVIVDIDVTKTYNDFMGYSGREHLSAEDFY
                     SNSLLFDFEKGKINVEEFLVGLAGILNSSVEKEQLVKSWNSMIVDIPHDRLMLVNKIR
                     NYVPVFAFSNTNALHINHLNNLLLTKHGHSNLNSFFTKTFYSHEIGQRKPDLESFEWV
                     IREGEMEGKNVLFLDDNISNIIAAEKAGIKGWHITSPDEWIDYFSKWMK"
     gene            1492..2718
                     /locus_tag="VGA21_10440"
     CDS             1492..2718
                     /locus_tag="VGA21_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017732547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEX9650969.1"
                     /translation="MDEVEQKKEKSGNWIHLLLFGITLITTTLAGAEWMFGRSLFGTY
                     SLSGLEILSGLEFSLPFLSILTVHEFGHYFTARHYNIKTTLPYFIPFLPITFSIGTLG
                     AVIRLKERVKSTKENFDIGIAGPLAGFVVAIFVLIYGFTNLPPKDHIFSIHPEYQLLG
                     ENWESLVYDTDTIVVNPKGRYPESELLNLLPDTIEFSTQIPKVYLGTNLIFEFFKNNV
                     ADKERLPNHYEAMHYPWLFAGFLALFFTALNLIPVGQLDGGHVLYGLFGRENHRIISS
                     GIFIAFVFYAGLGVINPFDPNVGFAETVLYSILYVGFLYLTYYTISKQFRDRLFIAVL
                     TFTAQFGVVYLFPGVIGYQGWLIFAFIIGRFLGIQHPPTLYDEPISFNRKILGWIALL
                     IFVLSFSPRPIIVEGI"
BASE COUNT          784 a          498 c          552 g          914 t
ORIGIN      
        1 gttagtaaat tgaaagcaga ttccatcact tccttttcag cttccaatac caggtaatgt
       61 ttgaaagatt gatgacgcat gaactcgcta atccaatttg tatcccatac acaaaatgtg
      121 gcaaaaggaa attccttcct aagtctattg tatagtttct tcaagttcct ggatggctcc
      181 ggtttatagc ttgtctttgt tcccaaagaa taaatgcctt ttgatggatt tcgtataata
      241 ccctgcttct taagttgaga aatccgaatg tctatcgtag cctcggacca gcttgggaaa
      301 tcacgcgaga tcgctttagc tagtttctgc ttggagatct ggcctttctt actcaaatac
      361 tttcttatgg tatcttctag ctttttattc aaatttagtg attaattcat tatatataag
      421 attggcaaat atagccagtt tttgccaatc ttatatactc atatgcagca ataccactta
      481 attttactaa tttatgtttc ttacttaaga acaagatgaa agattgagtt ataactttcg
      541 ttttaggaac gaccatcaaa aggtttagag attttaaaag aagtagtttt tcgcctgagg
      601 tcttgattct agtcattcat caaagtcctc tttgcgagag actctgatgg gattatttgg
      661 aaaactcttt tgggaaatgg agaaaacaat tgataattcc taattgctaa ttgaaaattg
      721 attcgtgggc ccagcaggat ttgaacctgc gaccccctga ttatgagtca gatgctctaa
      781 ccaactgagc tatgggcccc aaggcagtca ggccttgtaa ataggagtgc aatgttacat
      841 atttggggct tattgtacaa aaatattgaa tgttttggat gccgtaaccc ctgaagcaat
      901 aatctttgat ttgggaggag ttatcgtcga catagatgtg acgaaaacct ataatgactt
      961 tatgggttac tccggacgag aacatttaag cgcggaagat ttttattcaa attcattatt
     1021 gtttgatttt gaaaaaggga aaataaacgt tgaggaattc ctggtcggac tggccgggat
     1081 tcttaattct tcagtcgaaa aggagcaact tgttaaaagc tggaattcta tgattgtgga
     1141 cattcctcac gatcggctta tgctggtgaa taagattagg aactatgtgc cggtctttgc
     1201 attcagtaat accaatgcgc ttcacattaa tcacctgaat aatttactac ttacaaaaca
     1261 tggtcattcg aacctaaatt cattctttac caaaactttt tattcacatg aaattggaca
     1321 aaggaagccc gaccttgaat cctttgaatg ggtgataaga gaaggagaaa tggaagggaa
     1381 aaatgtttta tttttggatg ataatatatc caacataatt gcggctgaaa aagccggaat
     1441 aaaggggtgg catataactt ctccagatga atggattgat tattttagca aatggatgaa
     1501 gtagaacaga agaaggagaa gtctggaaat tggattcacc tcttgttatt tggaataact
     1561 cttataacca ctacgcttgc gggagccgag tggatgtttg gccgatcgtt gttcggaacc
     1621 tactccctca gcggattaga aattctgtcc ggccttgagt ttagtctgcc tttcttatct
     1681 attcttacgg tgcatgagtt cgggcattat tttactgcca gacactataa tatcaaaaca
     1741 actcttcctt atttcattcc ctttctgccg atcacatttt ccattggtac tttgggggca
     1801 gtcatccggt taaaagaacg agtaaaatct acaaaggaaa atttcgatat aggtattgca
     1861 ggtcctttag cgggatttgt ggttgctatc tttgtcttga tttatggatt cacgaacctt
     1921 ccaccaaaag atcatatatt ttcaattcat ccagagtacc aactcctggg agaaaattgg
     1981 gaatcccttg tttatgatac ggatacaatt gtagtgaatc cgaaggggag gtaccctgaa
     2041 agcgaattat taaatctatt gcctgacact attgaattct ctacccaaat acctaaagtt
     2101 tacctcggga ctaacctgat atttgaattc tttaagaata atgttgccga taaggaacga
     2161 cttcccaatc attatgaggc gatgcattat ccctggttat tcgcgggctt tctggcactt
     2221 ttttttactg ctctaaacct tattccagtt ggccagctgg atggagggca cgttctgtat
     2281 ggacttttcg ggagggaaaa tcataggata atttcgtcgg gcatattcat cgcttttgta
     2341 ttctatgccg gccttggagt gattaatcca tttgatccca atgttggctt tgcagaaact
     2401 gtattataca gcatccttta tgtcggattc ctttatttaa cctactatac catttccaaa
     2461 caattcagag acagattatt tatcgctgtt ctcactttca cggctcaatt tggagtggtg
     2521 tacttatttc ccggagtaat tgggtatcag ggttggctta tttttgcttt tattatcgga
     2581 aggtttttgg gaatacaaca tccgccgact ttgtatgatg aaccgataag ttttaacaga
     2641 aagattcttg gatggattgc attattaatt tttgtgctga gctttagtcc aaggccaatt
     2701 attgttgaag gaatctaaat ttggacaatt acattcctgt tccattca
//
LOCUS       DASWHX010000157         5377 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_540595, whole genome shotgun sequence.
ACCESSION   DASWHX010000157 DASWHX010000000
VERSION     DASWHX010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 5377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5377
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_540595"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..187
                     /locus_tag="VGA21_10445"
     CDS             <1..187
                     /locus_tag="VGA21_10445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650970.1"
                     /translation="GNFEEYLGRVYFDQKKIKRPINYPFSISFGWLLTVLLGNIHGET
                     MAKTNKTRTNNNADIGL"
     gene            447..1151
                     /locus_tag="VGA21_10450"
     CDS             447..1151
                     /locus_tag="VGA21_10450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650971.1"
                     /translation="MRNCNFPLIFTFLVSLSCGSRDNNQSESFSEIKGSNESNEKIEA
                     IPILIMTYPVESFSTWKSAFEEDKSNRESLNIVDLGDLVSYEDSRMVSVFLKTPSHKK
                     GEEFAKSDAMQKSLKLMGVIGDANIILIDALQLPERPVPQRYRMMITHEIEEYNHWKK
                     IFDQHEPERRDAGLELIGLGRSIINAKIISVMFAFDDMDAAQIFAASDELRNAMKESG
                     VISDPILAWYENPRAN"
     gene            1203..2618
                     /locus_tag="VGA21_10455"
     CDS             1203..2618
                     /locus_tag="VGA21_10455"
                     /inference="COORDINATES: protein motif:HMM:NF019284.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650972.1"
                     /translation="MKQVFVIAFNFLFHYSLCQVQIIFSPDSSTFAKGEVTNGRKTGT
                     WIVYNTSSKMPIEAGRYANDKRTGQWTSYYPNGDVRAQLAYEFDLPQGDFIELYEGGY
                     LKTTGQYDNGLPEGRWRNYHLNSLLESEYYYEMGELTGAHKAFSRTGNLFITANYQSD
                     LLHGLWHEYYDKGEIYERGFYLNGEKDSTWVSLDPYGDTLSIKNYNLGTPVGTWEEYA
                     PTGILKRRIYYRAGLEDSLYFENYDNGRAKVRGSFESGEKVNTWSFYFDNGMLESQGN
                     FQNNLKEGLWRSFTRNGYLVDEGNYSKGLQTGQWYYYYGNGQLSSIGSFIKGKEDGLW
                     GLFHTTGLLMQEEEWKMGKLINVSDYVSILGDTLSAGTLKNGSGLKIDYYPGGALNIE
                     CEFSEGVPNGNWTYYYDNGKVAEEGKFKDGLREGSWRYYYSNGKIESSGNYEKDKKIS
                     IWIYYSRRGDILDKINEFRKE"
     gene            2621..3166
                     /locus_tag="VGA21_10460"
     CDS             2621..3166
                     /locus_tag="VGA21_10460"
                     /inference="COORDINATES: protein motif:HMM:NF022588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HEX9650973.1"
                     /translation="MGRHITVFFGIIIAFIVAGLYGFRSYTKSFSPEGEANLTTRSGF
                     IVNINYSRPYQKNRKLFGFKDALAPFGKVWRTGANEATEIEISHDVEIYGQKLSAGRY
                     TLFTIPNEITWMVIFNNELNQWGDFNYDSSKDELRVAVPAFVVKTRKEMFTLEVREKP
                     DGAEIRLLWGDTMVTIPMVVN"
     gene            complement(3168..4898)
                     /locus_tag="VGA21_10465"
     CDS             complement(3168..4898)
                     /locus_tag="VGA21_10465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650974.1"
                     /translation="MRYYFLLSALLLVQLSFGQMAKLNPSINRVGSNNYFPTLSGDGK
                     YMLYRSDLSGEGEELWITKMTSSGQWGLQERFNRVIDLPGVNTMGGFYLNIDGSLLVF
                     SSRRYGSMGGFDIWTSERKGSSWSPPQNGGKPLNTNGHEGDPSLSPDGKTLYFIRCEG
                     LSFEEAINCSIWTARRKSNGFFEEPEKLPAPINTGHETSPLILADNQTLVFASARAGG
                     KGELDLYLSRKEGEEWSQPVSMEFLNSEKNDRYVSIPARGDVAYYSGIDKRQKVLYMA
                     KIPEGLRPYKVLYLESRVVSQSSNPIEATIQYSAINEPSKFVTAEEDGIFSLILREGK
                     IYDIGVLEREGKYLFASERIDLSEIEFSKKERKEYILNDIRNGAEQPLNNIIFQEYSS
                     ELDEASEKELQRLIFILQKNPDYNMEIAVHQKNYMADTIKSNPDLTELIIDTIHTTKI
                     LETVDTIWRKIDFESLLHDPEIDYIMVDSVLLTNPREADRVLDSLNNAISNPERNFET
                     ITNYDTLQVTKIKYTWHNDRSQQQAISIANYLKSKGAPLYRLSTRGMRTKKEAEEDKP
                     EVRVVARFLK"
     gene            complement(4970..>5377)
                     /locus_tag="VGA21_10470"
     CDS             complement(4970..>5377)
                     /locus_tag="VGA21_10470"
                     /inference="COORDINATES: protein motif:HMM:NF022441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2807 domain-containing protein"
                     /protein_id="HEX9650975.1"
                     /translation="LYAGGAIELRSRNPLRSRYFDLNLAGAGEIILEFEGEKLNLRLS
                     GAGNVELSGSTRDLEVTMSGAGGLDAFDLKAEYARIRISGVGGADIYVTKELDAKISG
                     LGDVTYRGNPANIDKSVSGLGTIQEDRHFSDDQ"
BASE COUNT         1608 a         1063 c         1074 g         1632 t
ORIGIN      
        1 gggaaatttt gaagaatatc tgggaagagt ttatttcgac caaaaaaaga ttaaaagacc
       61 tataaactat cctttctcaa tatccttcgg atggttactc accgtcttat taggaaatat
      121 ccatggagaa acaatggcta aaacaaacaa aacgagaaca aataataatg ccgacatcgg
      181 gttatgattt taattcaaat ataaagagac tctttcaagt ttcaaaaatc ggtagtgtct
      241 tagtggagca ttttgcgtgt gccacatttt gccaaacatt gaaaatcaat cagttttcac
      301 ctaaaataaa aatttggaag tgtagcacac gttgatttta atttcatttg gatttaaact
      361 gaggcactat tccaaaaatc agcagcccgg attgttatcc gctcaaaaaa agtttaactt
      421 caacattaac tggacctgtt cttttgatga gaaattgcaa ttttccattg attttcactt
      481 tccttgtctc cctttcttgt ggttctcgag ataacaacca aagtgaatcc tttagcgaaa
      541 tcaaaggatc taatgaatct aatgagaaga ttgaggctat tcccatcctt attatgacct
      601 atccggtgga atctttttct acctggaaat cagcttttga agaagataaa tccaataggg
      661 agtctttgaa cattgttgat cttggcgatt tagtctccta cgaagactcc cgtatggttt
      721 cggttttcct gaaaactccc agtcataaaa aaggggagga gtttgcgaaa tctgatgcaa
      781 tgcagaagtc actaaaatta atgggagtga ttggtgatgc caacattatt ttgatagatg
      841 cactgcaatt gcctgaaaga ccggtacccc aaagatacag gatgatgatc acgcacgaga
      901 ttgaagagta taatcactgg aagaagattt ttgatcagca tgagccggaa agaagagatg
      961 caggccttga gctaattggc ttaggcaggt caataattaa tgcaaaaata atcagcgtta
     1021 tgtttgcctt tgatgacatg gatgccgctc aaatttttgc tgcctccgat gagcttagaa
     1081 atgctatgaa agagtctgga gtaatttctg atccaatcct tgcctggtac gagaatccgc
     1141 gtgctaatta attgtcaaat tatagcatta attttgctta tcttttatga gagattcatc
     1201 gaatgaaaca agtctttgtt attgccttta atttcctttt tcattactca ctttgtcagg
     1261 ttcaaataat tttcagcccc gacagctcaa cttttgccaa aggtgaagta actaacggcc
     1321 gtaaaaccgg cacctggatc gtttataata ctagtagcaa gatgcccatc gaagccgggc
     1381 gctatgcaaa tgataaaaga accggtcaat ggacttctta ttatccgaat ggggatgtta
     1441 gagctcagct cgcgtatgaa tttgatcttc cccagggaga ttttattgaa ctctatgagg
     1501 gaggctacct taaaaccaca ggccagtatg acaatggact tccggaaggc agatggcgaa
     1561 attatcactt aaacagtttg cttgaatcag agtattatta cgaaatgggc gagctaaccg
     1621 gagcccacaa ggctttcagc aggaccggca acctttttat tacagctaat tatcaatcgg
     1681 atttgctcca cggcctgtgg catgaatatt atgataaagg ggaaatatac gaaagaggat
     1741 tttatttaaa tggtgaaaaa gacagtacct gggtatctct tgatccatat ggtgatacgc
     1801 tttccataaa gaattacaat ctgggaacac ctgtaggaac gtgggaagag tacgcgccga
     1861 caggtattct caaacgcagg atttattacc gggcaggact tgaggacagc ctctattttg
     1921 aaaattacga taatggccgt gcaaaagtca gagggtcatt tgaatcaggt gaaaaagtaa
     1981 atacctggag tttttatttt gataatggca tgttggaatc acagggtaat tttcaaaata
     2041 acttaaagga aggattatgg cgatcattta cccggaatgg ctacctggtt gatgaaggaa
     2101 attattcaaa aggtcttcaa acagggcaat ggtattatta ctatggaaat ggacagctta
     2161 gtagtattgg ttcttttata aaaggaaagg aagatggctt atggggattg ttccatacca
     2221 ccggtctgct aatgcaagag gaagaatgga aaatgggaaa gctgataaat gtttcagatt
     2281 atgtttctat cctgggcgac accctgagcg ccggcaccct gaaaaatgga tcaggtttaa
     2341 agatagatta ctatcccggc ggtgctttga atattgaatg cgaattcagt gagggcgtac
     2401 ctaatggtaa ttggacctat tattacgata atggcaaagt tgctgaagag ggcaaattta
     2461 aagatggttt aagggaaggg tcatggagat actattattc aaatggaaag attgagtcat
     2521 cgggtaatta cgaaaaggat aagaaaattt cgatttggat ctactactcc cgcaggggag
     2581 acattcttga taagattaat gaatttcgaa aggaataaaa atgggccggc atattacagt
     2641 tttctttgga atcatcattg cattcatcgt ggcgggtctg tacggttttc gttcatacac
     2701 aaaatccttt agtcctgaag gtgaagccaa tttaaccacc agatccggat ttattgttaa
     2761 tataaattac tccagaccgt atcagaaaaa cagaaagctc tttggtttta aagatgctct
     2821 ggctccattc ggtaaagtct ggagaacggg agctaatgaa gccactgaaa ttgagattag
     2881 ccacgatgtc gaaatttatg gacaaaaatt aagtgccggc cggtatactt tattcactat
     2941 tccaaatgaa atcacctgga tggttatctt caataacgaa ttaaaccaat ggggagattt
     3001 caattatgac agcagcaaag acgaattacg tgttgcagtc ccggcctttg tggttaaaac
     3061 ccgcaaagaa atgtttacgc tggaagtcag ggaaaagccg gatggtgcag agatcagact
     3121 tttgtggggc gataccatgg ttaccattcc aatggtagta aattaaatta cttaagaaac
     3181 ctggcaacaa cgcgaacctc gggtttatct tcttccgctt ctttttttgt tctcattccc
     3241 ctggttgaca gccggtataa cggtgcacct tttgacttta aataatttgc aatggatatt
     3301 gcctgttgtt gcgaccggtc gttatgccag gtatatttga ttttggttac ctgcagcgta
     3361 tcgtaattag tgatggtttc aaaattccgt tcaggattcg aaatggcatt atttaatgaa
     3421 tccagaaccc tgtctgcttc acgagggttt gttaataaaa ctgaatccac cattatgtaa
     3481 tctatttcag ggtcatgaag tagtgactca aaatcaattt tgcgccatat agtatccact
     3541 gtctccaaaa tttttgtagt gtgaatggtg tcaattatca actctgttaa atcaggattt
     3601 gattttattg tatccgccat gtaattcttc tggtgtacag caatttccat gttataatca
     3661 ggatttttct gcaagatgaa aattaatctc tgtaattctt tttctgaggc ttcatctaat
     3721 tcagagctgt attcctgaaa aatgatatta ttcagaggtt gttcagctcc attcctgata
     3781 tcatttaata tatattcttt cctttctttt tttgaaaact caatttcaga taagtcaatc
     3841 ctttcagaag cgaataaata ctttccttct ctttctaaca ccccgatgtc gtatatcttt
     3901 ccttcacgaa gtatcaaact aaatattcca tcttcttcag cagtgacaaa tttagaaggc
     3961 tcgttaattg ccgaatactg tattgtcgct tcaataggat tactgctttg ggaaacaacc
     4021 cgtgattcaa gatacaatac cttgtatggt ctgagtcctt ccgggatttt cgccatgtat
     4081 aaaacttttt gtctcttatc aattcccgaa tagtaagcca cgtcaccccg ggccgggatt
     4141 gaaacatagc gatcgttttt ttcagagttg agaaactcca ttgaaacagg ctgcgaccat
     4201 tcctctcctt cctttcggga aagataaagg tctagctcgc cttttcctcc tgccctcgcg
     4261 gaggcaaaaa ctaaagtttg attgtcagca agaattaatg ggcttgtttc atgacctgta
     4321 ttgataggtg caggcagttt ttcaggttcc tcaaaaaaac catttgattt tctcctggcg
     4381 gtccagatgc tgcagttgat tgcttcttca aaactcaatc cttcacacct gataaaataa
     4441 agggttttgc catccggtga tagtgagggg tctccttcat gaccattggt gttcaatggc
     4501 ttgcctccat tttgcggggg actccatgaa cttcccttcc tttcggaagt ccatatatca
     4561 aacccaccca tgcttccata tcttcttgaa gagaaaacca aaagactgcc atcaatattc
     4621 agatagaaac cccccatcgt gtttactccc ggaagatcaa tcactcgatt aaatctttcc
     4681 tgcaatcccc actgacctga agatgtcatt ttagtaatcc aaagttcttc accttcacct
     4741 gacaaatcac ttctgtatag catgtatttt ccatcaccac ttaatgttgg aaaataatta
     4801 ttggaaccga ccctgtttat tgatggattt aattttgcca tctgtccaaa agataattga
     4861 acaagcagca aggcggataa aaggaaataa tatcgcataa attaagattc tcaccttaag
     4921 ttataccatt ttatttaaag gtcgaatcca ttattccgct aaaatcggat tattgatcat
     4981 ctgagaaatg acgatcctcc tggattgttc ccaatccgga aacactttta tcaatgttcg
     5041 cggggtttcc cctataagta acatcgccca atccacttat tttggcatcc aattctttgg
     5101 ttacataaat gtcagctccc ccaacaccgc ttatccgaat tctggcatat tccgctttga
     5161 gatcaaaagc atcaagccca cctgcacccg acatggtaac ctccaaatct cgagtggatc
     5221 cggacaattc cacattcccc gcgccactca gcctcaggtt tagtttctct ccttcaaatt
     5281 ccaggataat ttctcctgca ccggcaagat tgagatcaaa gtatctcgac ctgagcggat
     5341 tccgtgacct aagttctatc gcgccgcctg cataaag
//
LOCUS       DASWHX010000158        54979 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_2400369, whole genome shotgun sequence.
ACCESSION   DASWHX010000158 DASWHX010000000
VERSION     DASWHX010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 54979)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54979)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54979
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2400369"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            159..2456
                     /locus_tag="VGA21_10475"
     CDS             159..2456
                     /locus_tag="VGA21_10475"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HEX9650976.1"
                     /translation="MIHQEEKLLYDKSQEIDWLKLRMILQKSLIWMLLIIFTTNSTAY
                     LYLRWTKPVFVSESNLKLDIKSEAGILGFDAIDEEQSLGTISGEIELIKSRMFSERVI
                     KSLDLGVSYYSVGKILNDERYKFSPFRINVINMDPNLFDTPVYVEFQENGDFSVSLSI
                     ESLADKMVFSSIDTIRVSNLEFTLNIIKPLNRETLETKFFFLVNSYQKLITYLETNLF
                     VEPVNLSAKTIKVSFRDHNPYKAMDLVNSIDTLYLNYSKEEKQRANFQKIKFVDQQLL
                     ATDEKLSQIEDYLESETLGLQGFSLDNESNEVISRLKEVKSELIAIHKQHQESYSLVA
                     GLDNGDSVWIPKFMAIHPEIIQEITDFKNSKLKLSKLRKTFSENTRIVLEEKELAEMR
                     KRNLVLSLDKLIGSQEILLGELTMERNSLSKKLTSIPQRKNDFNKTNRYYTMYEDFYL
                     SLMQNKTQFQIAQAGITTDFKILSQASIPASPVSPRKTIIFGIGFMSGFLICFFYIGI
                     RYVLHNRITSVEQIESKTKLPVLGSIPFYHSEKLKASKLLVGKHPRSAISESLRGIRT
                     NLEFFLPSKKNKILAVTSTVSGEGKTFFSVNLAGIISLSSKKVIVVDLDMRRPRVHQA
                     FGIECPKEGVSSILIGKNSLEECIHITSMHKLHFLSAGPPPPNPSELLLKPEFDELLK
                     KLSMLYDVIILDTPPVGLVTDGILALKKADLPIYVLRSDFSKTHYLETLMRLNKIHKF
                     SNLTVLLNSIKMSSQGYGYGYGEGY"
     gene            2498..3217
                     /locus_tag="VGA21_10480"
     CDS             2498..3217
                     /locus_tag="VGA21_10480"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007653498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsB/CapC family capsule biosynthesis tyrosine
                     phosphatase"
                     /protein_id="HEX9650977.1"
                     /translation="MNLFSRSKPEKILEVDMHSHLIPKIDDGSQSTRETIEMLKFMHE
                     MGIRKVITTPHIISDLYPNTPAKIMDSFSLLNQEIAGENIPIELEVSAEHYLDEFLMQ
                     SLDRNDILPFGGKYLLFETSFMLKPAVLEDFIFRVISKGLKPVMAHPERYVYFQDSWD
                     LLTEIWNRGALFQINISSLGGFYSRPAKVVAQKLIDSGRVHFLGTDAHNMEHLKKCAI
                     VMKNKYVQKALHLPLLNFELS"
     gene            3214..4182
                     /locus_tag="VGA21_10485"
     CDS             3214..4182
                     /locus_tag="VGA21_10485"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX9650978.1"
                     /translation="MILITGANGLVGSFICRKYLDAGFEVSILVRPSSDLTFLKDIEG
                     RIKIHYGDILDVLSLEQAMQGCKTVVHSAALVSLLGKERKKMFKVNIEGTKNVVNTCL
                     KMGVEELIHISSIAALGRIKEVIQIDENAKWTQSKLNSGYAETKYLSELEVWRGMEEG
                     LNIIIVNPSVVLGIGNWNKSSGQIFKYVHDGNAFYTKGKVNYVDVRDIADAVFILHQN
                     RIYGERFILNAGVSTYKDLFNLIAKELGKKAPLFRVGTILLWMAMVFDQLKSLFTRKS
                     PLITRETVILSEMNFFFDNKKIIENTGIDFRPLQETVRWVCGSQTP"
     gene            4250..5656
                     /locus_tag="VGA21_10490"
     CDS             4250..5656
                     /locus_tag="VGA21_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009579769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9650979.1"
                     /translation="MSESFKRRREDNELIRKFEDHLKNDRSFYFDVDSYEEIIDYYLE
                     RGKYNYALRACTLAIEQYPYSTDILLSKAEILANLEDYDEALKAIDNVLIFQPTDSEV
                     LSLKANILMQAGYYERAVEAFRETIIYGDQSGELYYGLGLAFQSLEKYSEAIDCYKKA
                     IDINPKHENSLYELAYCLDVKGELESSVAYYKQFIESDPYSHFAWYNLGILFEKMENY
                     EEAIKAYEFATAIDESFASAHYNMGNTYMQLSKYSKALDAYKKTFDKEGASADIYFSI
                     ATAYEKLDQFELGLKYYRKAVKLDIFYDDAWFGAGRCLENQGKFYEAIHFYNKALKLD
                     NENPEYWKSVADMEFKVGNLVSSLTAYEEASTRDPENMAVWLDWSFVYYEQGDYEKAM
                     SLVKEGISLIPESSDLYYRATAYLVSGGKYQEAFAYLENALILNFDNHTVLFEFFPKL
                     ETQKALFRIIDQFRKENS"
     gene            5663..6436
                     /locus_tag="VGA21_10495"
     CDS             5663..6436
                     /locus_tag="VGA21_10495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019989717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphosulfolactate synthase"
                     /protein_id="HEX9650980.1"
                     /translation="MNYFLKDLPERTQKPRQHGFTMAMDKGLSVREAEDFLSVCSEFV
                     DIVKFGWATTYVTPNLIDKIKVYKAAGVPVYFGGTLFEAFIVRDQFDDYRKILDKYEM
                     EFCEVSDGSIELDHEKKCEYIAKLKEQVTVLSEVGSKDAEKIIPPYLWIKLMQEELDA
                     GAWKVIGEAREGGNVGLFRSTGEVRSGLVEEILTKIPFEKIIWEAPQKSQQVWFIKLL
                     GANVNLGNIQPSEILPLETIRLGLRGDTFNLFLNKNNSR"
     gene            6442..7380
                     /locus_tag="VGA21_10500"
     CDS             6442..7380
                     /locus_tag="VGA21_10500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018472464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HEX9650981.1"
                     /translation="MSWLKKYPVIYLKGIAMGIADAIPGVSGGTIALIAGIYEQLIDS
                     IKAFDITAIKYLRKFQIRDFWRYVNGNFLLTLVLGIATSLVTLSHFVTFLLREYPIQI
                     WSFFFGLIIISSAVVLRRINKWNPGVIISLLIGVAAAFLITKATPAQTPNELWFIFIS
                     GSIAIIAMILPGISGAFLLLLLGKYEYVYLALRDLNLAIIGVFIAGAITGLLSFSRAI
                     SWLLKNYHNSAIALLSGFMIGSLNKIWPWKIITLFRENSEGEQIPLLEKNVLPAQYFQ
                     ETGNDPFIFQAILFVAVGIFIVVLLEKISISRNKST"
     gene            7377..8123
                     /locus_tag="VGA21_10505"
     CDS             7377..8123
                     /locus_tag="VGA21_10505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013454793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEX9650982.1"
                     /translation="MKLLGLIGHPLTHSFSADYFNKKFERLKLTDFNYALFDFIEISD
                     IRRWAATHPDLLGLNVTIPFKEKIIPYLDQVDKNAKKIGAINTIRIYNDTWKGYNTDY
                     IGFRESLKKIIRSESKLNALILGCGGSAKTVSKVLLDMEIQFQMISRKESKDALSYDQ
                     LNSGNFISDSRLIINTTPLGMHPDINSLPAIEYNQISDKHILYDLVYNPEKTAFLLEG
                     EKRGARIKNGLEMLYIQAEQSWKIWTNNLK"
     gene            8120..10162
                     /gene="uvrB"
                     /locus_tag="VGA21_10510"
     CDS             8120..10162
                     /gene="uvrB"
                     /locus_tag="VGA21_10510"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009579776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HEX9650983.1"
                     /translation="MNFQLTSEFEPTGDQPEAIQGLVNGLTSGENFQTLLGVTGSGKT
                     FTIANVIQKLQKPTLVLCHNKTLAAQLYGEFRQFFPNNAVEYFISYYDYYQPEAYMPS
                     TNIYIEKDLSINEEIEKLRLSATSSLLTGRRDIIVVASVSCIYGIGNPDEFEKNVISL
                     TVGERIPVSKLLYNLVDILYSRSDLDFKRGTFRVKGDTVDIFVAYADFAFRIFFWGDE
                     IENIQVIDPATGEKVAEEKSVSIFPANLFVTGRDVIDVAIKEIQEDLVMQLNLFEEEG
                     KVGEAKRLKDRTEFDLEMIRELGYCSGVENYSRYFDRRKAGARPFCLIDYFPDDFLLV
                     VDESHATIPQVRAMWGGDRSRKVNLVDYGFRLPSALDNRPLTFNEFEAMLNQVVFVSA
                     TPSDYELRKSEGVIVEQIIRPTGLLDPQIEVRPSANQIDDLLEEIDERVKVSERILVT
                     TLTKRMAEELSKYLDKASVKSRYIHAEVMPLDRVEIIRELRLGIFDVLVGVNLLREGL
                     DLPEVSLVAILDADKEGFLRNVRSLVQTIGRAARNERGKVIMYADVMTDSMRVAIDET
                     NRRRNIQQEYNRMHGITPVTIKRSRESIMGQTRVADSLKNKKKFYVEPESKSVAADPI
                     IKYLDESKLETLIKDTRKKMELAAKDLDFLEAARYRDELYELEDFKEKKKNKSHEK"
     gene            10152..10574
                     /locus_tag="VGA21_10515"
     CDS             10152..10574
                     /locus_tag="VGA21_10515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650984.1"
                     /translation="MKNKLFLIVFILTAQWCVAQSSKSFVISVGGDVIKTDIQNFADK
                     VQTGAELNYYLLRNFTVTAGFEFWSSGRESAVLGMRYYPHRNIFARFRGLIGANQVAL
                     GAGYSYPLNERFKLDGLGDFYFDSAEFAFRVGFSYVIR"
     gene            complement(10655..11263)
                     /locus_tag="VGA21_10520"
     CDS             complement(10655..11263)
                     /locus_tag="VGA21_10520"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014772102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEX9650985.1"
                     /translation="MAFELPKLPYSYDALEPHIDAQTMEIHHTKHHSAYVTKLNEAIA
                     GTDNEGKSLEELMNKVSGLSPAIRNNGGGHYNHSLFWTVLSGNGGGTPSGELSDAISK
                     AFESFDKFKEQFSNAGATRFGSGWAWLGIKANGGLCVCSTPNQDNPLMDVATDPCTPI
                     LGLDVWEHAYYLKYQNRRPEYIASFWNIVNWEEVSRRYSEAK"
     gene            complement(11308..11757)
                     /locus_tag="VGA21_10525"
     CDS             complement(11308..11757)
                     /locus_tag="VGA21_10525"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017730243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEX9650986.1"
                     /translation="MTVHPKTDEFFMKEALKLAKEAFTKKEIPVGAVVVMDGKVIAKA
                     YNQTEQLNDSTAHAEMIALTSAFNHIGAKYLKEATLYVTLEPCVMCGGAIFWSQLGRL
                     VYGATDEQKGYRKTSSKILHPKTKIKGGVLGEECKSLIDAFFRQIRN"
     gene            complement(11759..12295)
                     /locus_tag="VGA21_10530"
     CDS             complement(11759..12295)
                     /locus_tag="VGA21_10530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650987.1"
                     /translation="MKLSLFIFLLFTPFPGEKFVKVEVSEGITVKIPESYAEMSSQDL
                     EQRSQSYRRPVAIYTDPSRTAEFVVNFSFSLWNTQDLELLMRFYKASLYEIYSEITMI
                     TEEIQTINKREFVVLEFYSVSKEEKAENLRPIRKYTNLRYTLDQGRTILFNFTCPQNL
                     QKDFEDTANQIMESIRIK"
     gene            12401..14164
                     /gene="aspS"
                     /locus_tag="VGA21_10535"
     CDS             12401..14164
                     /gene="aspS"
                     /locus_tag="VGA21_10535"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020532802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEX9650988.1"
                     /translation="MYRTHTCGELRVSHKGINTTLAGWVQKIRDKGGMIWIDLRDRYG
                     ITQLILEEGKSDNKLIEQARELGREDVIKIAGIVQERYSKNQNLATGEIEVLLSQLEM
                     LNESNLPPFIIEDETDGGEDLRMKYRYLDLRRGPMKENLRLRHRLMNYTRDYLTRNQF
                     IEVETPVLIKSTPEGARDFIVPSRINQGEFYALPQSPQTFKQLLMVGGLDKYFQIVKC
                     FRDEDLRADRQPEFTQIDCEMSFVHRDDVLSMFEGLIKFLFKEEKGVDLADFGRMTYA
                     DAIANYGSDKPDLRFDMSFIELNSLVKGKGFKVFDDAELVVGILAKGCAEYSRKQLDE
                     LTDFVKKPQVGAKGLVYVKFQQDSTIKSSVDKFYNELDLQNWKKNCSAGNGDLLLIIS
                     GDAKTARTALSTLRLEMGNRLNLRDSNTYKPLWVLDFPLLEWEEESGRYFAMHHPFTS
                     PKPEDLAKLESDPGSVNANAYDLVINGVEIGGGSIRIHDRSVQQRMFNILGFSKEEAQ
                     RQFGFLLEAFTYGAPPHGGIAFGFDRLCALFGGTESIRDYIAFPKNNAGRDVMIDTPS
                     QIHKSQLDELGITVLSKKKKE"
     gene            complement(14511..15587)
                     /locus_tag="VGA21_10540"
     CDS             complement(14511..15587)
                     /locus_tag="VGA21_10540"
                     /inference="COORDINATES: protein motif:HMM:NF015647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HEX9650989.1"
                     /translation="MNSVYNVIGLMSGTSLDGLDIAGVMLTDHNNNWSFEILCCESIA
                     YSQTIKNTLAKATQSSAIDLAEMDLALGDFFGNAVNRFITKNNFKPDFISSHGHTVFH
                     QPKKRLTTQIGSANRVHALTGVPVIYDFRSLDVQLGGQGAPLVPVGDVLLFSSYDACL
                     NLGGISNISYDFNGQRIAYDVCALNIIFNYYSRILGFELDESGQMAREGSVNPELSNF
                     LTNLDYYQLNAPKSLGFEDIEKDILIPVKAFEVSANDCLRTFAEHFSDILADSIFRVE
                     RIKKILVTGGGVFNLYFIELLKKKLGGIEVCIPDRKIIEFKEALVFALMGALRLRGED
                     NVLASVTGASRNSCSGSIIGVVGL"
     gene            15640..16353
                     /locus_tag="VGA21_10545"
     CDS             15640..16353
                     /locus_tag="VGA21_10545"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HEX9650990.1"
                     /translation="MNAVIDIGNSRIKLGLFQNGKMIDCKLANNEDEASLLLGKIEIK
                     KMIVSSVRKENDWINNLKVGEKKVFLGPETPLPINIVYDTPDTLGTDRIAAATGAFLR
                     YPKKNCLVIDLGTCITYDFLDEKGNFNGGAISPGIRMRLNALHQQTSNLPLIEAWDAK
                     IPLTGKSTKESILSGVIIGVKQEIEGFIRSYHDKVANLQIIFTGGDFKMVENLLESRP
                     IWANHLVLEGLHGILEHNN"
     gene            16328..17611
                     /locus_tag="VGA21_10550"
     CDS             16328..17611
                     /locus_tag="VGA21_10550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650991.1"
                     /translation="MEYWNTTIRKRSFKILFVGFLLTHSLISAQTISTYSGLGLGDII
                     RPAFSNHIGNGLGLSKGEYWFVNQMNPALLPYNQVTTFQAGLGFETKTVSQNDLQDTY
                     SSFNLLYLGTAIPAKAGKWTFAFGVQPYSRVLYNFATSIDIPGSGVTAEITNQGSGGF
                     NQAYFSNGFKIWKNLSLGIKVSYLFSSLIKESTTIIGDTLDPSRSARSVLQRTNAKDV
                     IFSTGISYKHNINKDISANYGITFDPETKISAERFERIDIRTPAGTVLDIDTVANNLS
                     GSFRLPQKWGAGISFNEGFKWSFGAEVEYQQWSRFKDFSGNNDYFQDGFSIRVGGDIV
                     PNPIAIENYLNRITYRLGLSYDKLPFTISGKDIAEYGVNFGWSMPVSRISYIDFGMRF
                     AVRGSTRNNLIRENYFQLHLGFTFNDRWFLRTAYD"
     gene            17618..18919
                     /locus_tag="VGA21_10555"
     CDS             17618..18919
                     /locus_tag="VGA21_10555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650992.1"
                     /translation="MKKLLLCVLLAAETTVSFGQNCNNWNYPEDKKTVEEKLVLYSDN
                     KRFGNFKESAKHLNWLLVNAPGLHLSIYQNGSQIYEELANAETDPLRKRIYKDSSLLI
                     YDLRIQFCGDEKNVLNRKSLAAYHFFKDDKQRLGELYELFKKTFDLNKNSVWENILVA
                     YLDVVRRYRSAGGNISDEMVLEVYDEISGIIENNINEGGSFEKYEKYQEMLFQILLLI
                     IPLDCDFIDSNLVPRLKANPDDFDLSKKIVQLSVIAKCTETDFFIDAAKKVQAREPTY
                     GLAWAIGNRCLGNENFTCAVQFFEQAITLTDEKEKIADLYYKLANVAFRQDDYPKARE
                     LAFKTIETDHSRRDAYSFIGKLYADSYERCKEEVDRVKDRLVFIAAYQMFQKAGDMEA
                     MKFAKEQFPSREELFERNYNAGQSMKLTCWIDETVILQTRD"
     gene            18888..19493
                     /gene="lptC"
                     /locus_tag="VGA21_10560"
     CDS             18888..19493
                     /gene="lptC"
                     /locus_tag="VGA21_10560"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HEX9650993.1"
                     /translation="MKQLYFKQGINILKVKSLTFIFWSTSFFLLCSGCEEKIDPGKFV
                     EYEGPVTTLDGAEIMHSDSAIITAKIITDKLLQFTNGDQEMPSGFHITFFDSEGKPNA
                     TLRADYAYNDKQKNLWKATGAVELNNIKTGEKLNTEELFWDPPGERIFTEKFVRIESE
                     DQILLGEGLTASQDFTTYKIHKLTGEIVFNLDGEPSANPRK"
     gene            19490..19657
                     /locus_tag="VGA21_10565"
     CDS             19490..19657
                     /locus_tag="VGA21_10565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9650994.1"
                     /translation="MKIRNTLLYSASIGIFAIGIHQGLTVGWAYSYWIFMISVSFLLI
                     LKVLTNKEKER"
     gene            19662..20951
                     /locus_tag="VGA21_10570"
     CDS             19662..20951
                     /locus_tag="VGA21_10570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009579165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEX9650995.1"
                     /translation="MDPLPIIIVSLIFSALFSGLEIAYVTSNKLALELLGKQNTLIGR
                     VISVFQNNPSQFIGTTLVGNTLALVVYGIFMAKILEPVFAANFPAALQNQASILVVQT
                     IVSTIIVLVTAEFTPKSIFLINPNRMLSLLAFPMVIIYYLMYPVVFAIVKLSKLIITK
                     VFRREYYEDRPVFGLTDLNQFIKQLKESGLDEGKVGLDTKIFHNALEFKTVRVRECMI
                     PRTEITAVEVYDAMETVKKAFVDSGHSKVIVYRDSIDEVLGYIHSSELFKKPKSLEDI
                     ITPIIIVPETMLANELMIRFIKEHKSLSLVVDEFGGTSGLVSIEDIIEEIFGEIQDEH
                     DDEDLIEQQLDANTWLLSARHEIDYLNDKYGWEIPTGDYETLGGLILSAKEDLPKKDE
                     QVVIAPFRFTIITVQDIRIDTVKLHIDQKLRGLKQGS"
     gene            21019..23130
                     /locus_tag="VGA21_10575"
     CDS             21019..23130
                     /locus_tag="VGA21_10575"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HEX9650996.1"
                     /translation="MALINKLREKMGRVVAFAIGFAILSFILADLMGPSSFIMGGNNR
                     NIGEIAGETITLDEYQAEIEALVANYTMNLGRNPSEQEMATLRDQAWELLIVKHAFQK
                     QYEKIGLDVHEDEVVDMVQGKNVSPAIQQAFVNPETGDFDKQRLLAYLQQIKGAPQQQ
                     QYAWYLFEKDLKPSRLRLKYENLLMKSTYITTEEAKKNYENQTAVAEIKYLYVPYYSV
                     NDSLVSVTDKELETYLGAHSAQYQAQESRSFQYVTIPIEPSSDDTTYFREEIEELIGQ
                     IKNIEDDSVFAIINSDGLNPFREYRINELPGNVASRLDELEERMVIGPIFEGTAFKLF
                     KITSISEDTIFKARVSHILFKWEEESPEEKAKARKEAQDVLNQLQNGENFEILARLHS
                     KDNSASRGGDLGWGTKGQTWVAAFEAPVFNQKEGGLINRIVESEFGFHIIKVTEPANN
                     QIFKIATISKEVIASDETIEKAFRRAGLFQSSITDYDEFISKAKADSITLQTATGVTP
                     NQRNIPGLGQARVIVQWLYNTAKVGSISDVFELEGTYVVAVMTSRKVEGTSTLNDVRT
                     QVSLKVKNKKKADIIMSKLSEASGTLDEMATAYGSDAVVHSSSDLKLNTNFLPSVGLA
                     PEAIGKTFTLQGGERTIPIPTENGVVIVEMINFTLAGEIADYTTYKTQMKQGLTSGIS
                     YGLSETIKKQSDIKDERYKFY"
     gene            23160..23420
                     /locus_tag="VGA21_10580"
     CDS             23160..23420
                     /locus_tag="VGA21_10580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015264689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein"
                     /protein_id="HEX9650997.1"
                     /translation="MSEAFDDLRRTLDESYTWPVLYMFKFIAPKDKIDKLKTLFPKNE
                     FIERESKEGNYLSLTCKILVNSSEEVIRVYRQASKIEGVISL"
     gene            23436..24134
                     /locus_tag="VGA21_10585"
     CDS             23436..24134
                     /locus_tag="VGA21_10585"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEX9650998.1"
                     /translation="MKNKLAAILISNLLFFSCDSASNNRSEADVTNNSNARTEKSIDS
                     SAFELLVNKIEDPERKNWQNPDLVIQELGNLEQKTVVDIGAGTGYFTFRIAPLAAKVI
                     AVDIDKRFLDVIDDRKPDFDQEWVERIETRLTLEDDPGITENEADIVIIVNTYHFLKN
                     RVNYLQKVRNGMKEGGTILIIDFKQADMEVAPPGDLIVPSDTAIAELKASGFKNITVD
                     QMSLKYQYIIKATS"
     gene            24136..24375
                     /locus_tag="VGA21_10590"
     CDS             24136..24375
                     /locus_tag="VGA21_10590"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017729996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HEX9650999.1"
                     /translation="MWKEENNKLTRTFTFKNFTEAFGFMTKVAIEAEKMNHHPNWSNV
                     YNRVTIELSTHDAGNVVTEKDRKLAEIINKLVTEG"
     gene            24372..25076
                     /gene="rsmI"
                     /locus_tag="VGA21_10595"
     CDS             24372..25076
                     /gene="rsmI"
                     /locus_tag="VGA21_10595"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012926796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEX9651000.1"
                     /translation="MTGAKLFIVPTPIGNLGDITYRAIDVLSKVDLILAEDTRKTGVL
                     LKHYQISKPVKSYHNFNEHRQLAAIIEQLKSGRSIALVSDAGTPGISDPGYLLIRECL
                     NEKIEIEALPGPTAFIPALLKSGFPCNRFIFEGFLPHKKGRISRIEALKSEHRTIIFY
                     ESPHRLIKTLEQFSKIFGIDRAISISREISKMYEETFTGTVSEALDYFRSQTIKGEFV
                     IVVSGNQKKSDGNAPE"
     gene            25147..25593
                     /locus_tag="VGA21_10600"
     CDS             25147..25593
                     /locus_tag="VGA21_10600"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEX9651001.1"
                     /translation="MKTLQKNRENLPVMRSSLWDMFDFDRLFDRDLMNYQWFNKVPAA
                     NIVDKKDSFMINLAVPGMEKKDFHVKIDTGLLTISAEKEEKFEDKDEEFTRKEYNYNS
                     FTRSFVLPESVKTDGVQAKYENGVLNLTLPKKEEVKKSSKTEIKIN"
     gene            25659..26111
                     /locus_tag="VGA21_10605"
     CDS             25659..26111
                     /locus_tag="VGA21_10605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651002.1"
                     /translation="MQNLGVNYSYILETSLIDLRQKSKVWLSEVELWLIELAFFRKML
                     RLNKERLIEGNTSIENELDHYRELIDYYEVRLLRDLKKRLKKHEGKLVKLMDGHPVQD
                     EESFRENHKNIGQVVHAFGNEFRNLKKEFLTFIEPQKNNRYVSHINTD"
     gene            26086..26907
                     /locus_tag="VGA21_10610"
     CDS             26086..26907
                     /locus_tag="VGA21_10610"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX9651003.1"
                     /translation="MSRILIPIDFSECSLNALEYGLKYAEKTDPEPRIIIAHGYNIPI
                     PAAEFSMSLNATVLDEFKKDAEAKFEKLKERFPALKKNSVKFDVKMAFVLDAILKCVH
                     EENPHLIIMGTKGSSGINEVLFGSNTSGIIKSSKIPVIAVPENFKAGAISRITLAFDN
                     KELEDSSILEIVRRLSSIYHAPVDILHIQNKSEENDDGPAMLANFFEGVQHEFHLIEG
                     NDIAEEIEKFLDKSNSDLLAMIPKRHTIIERLFTRSITSKIAQHTRIPLLAIPIK"
     gene            complement(26900..27736)
                     /locus_tag="VGA21_10615"
     CDS             complement(26900..27736)
                     /locus_tag="VGA21_10615"
                     /inference="COORDINATES: protein motif:HMM:NF016362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP cone domain-containing protein"
                     /protein_id="HEX9651004.1"
                     /translation="MKQEISVVKNTGELEPFNKEKLIVSLKRSGASDEIIREINKEIS
                     KLLYPGIPTRKIYRVAFNQLRKKSILVASQYKLKEAITELGPSGYPFEVLVGKLFERM
                     GYQIEIGAILEGACVQHEVDVLAKKENMIEIIECKFHNRSSLKSDVKVPLYINSRYQD
                     IINFKNNNRSKFNFRGWIVTNTRFTDDAVRYSACAGLSLMGWDYPEKNNLKVKIGSSG
                     LYPVTVFTKLTRKEKTSLIENGIIFCGDIITRQNEVKNILRHKSLKSLLDEAISLHAT
                     AI"
     gene            27883..28266
                     /locus_tag="VGA21_10620"
     CDS             27883..28266
                     /locus_tag="VGA21_10620"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX9651005.1"
                     /translation="MSRQIQKIAEEEFRETGLSPSHAFVLMSVNARQEINPGEIAKEL
                     LLAPSTVTRLIEKLEALQLVQRENEGKYMKVITTSKGLEKDIRIRKAWENLFKRYTHT
                     LGQSFSTKLTENLQEAFSKSNRNKS"
     gene            28263..28907
                     /locus_tag="VGA21_10625"
     CDS             28263..28907
                     /locus_tag="VGA21_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009579909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEX9651006.1"
                     /translation="MKTVGILGSGPVGKALAKGFSKYGHQVMIGTRDKSRLDEWIVEN
                     PYAKSGTFDEVAKHGEILVLAVAGRVAKEVLNSSGRSNLSGKVIIDATNPISNDPPQN
                     GVLQFFTDLNHSLMEDLQTEYPDAMFVKAFNSVGNPFMVDPDFEEGKPTMFICGNDLK
                     AKSEVETILEKFGWEVEDMGGAEAARAIEPLCILWCLPGFLRNQWNHAFKLLKK"
     gene            29016..29750
                     /locus_tag="VGA21_10630"
     CDS             29016..29750
                     /locus_tag="VGA21_10630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009196259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HEX9651007.1"
                     /translation="MMISFLIYVFLLFPQTLDATDIIRRADQKMQGSSNMAVMTMVIQ
                     RPEWSREIEMKSWSLGIKKSLILITAPPRDAGTGFLKRDKEIWNWQPRIDRVIKLPPS
                     MMMQSWMGSDFTNDDLVKESSVVNDYNHTYKKDTVIRNYNCYKIEMIPKPETAVVWGK
                     VDVYIEKDELLQLLIKYYDEEGYLVNTMILSEIKNLGGRSIPTLMEMIPADEPNHKTI
                     IRYKEMSFDIDIDDSFFSLQNMKRVK"
     gene            29758..30975
                     /locus_tag="VGA21_10635"
     CDS             29758..30975
                     /locus_tag="VGA21_10635"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9651008.1"
                     /translation="MVLKLAWRNIWRNKRRTIITLSSIAFAVFFSVIMQSMQLGSYER
                     MIENVVKFYTGYVQIHSEGYWEEKILDNSFEPNSSLISEIARLEGVEQVVPRLESFAL
                     SSYQSQTRGALIMGFDPVAEKKLTALHAKIIEGEYLTENDNSVLLSQGLAEYLKIKVS
                     DTVVLIGQGYHAINAVGKYHVKGIIKFPAPAMNNQLVAMPILLAQWFYGAENKYTALA
                     LVLDSPDNVQKVSKEIVSILGRENLEVMTWQEMMPEIVQQIELDYVSGQVMLWILYAV
                     VAFGIFGTFLMMTNERRYEMGIMIAVGLRRIRMQMIMGTELLLMGFSGTLAGIIMAMP
                     LLIYFYFNPIRFTGEYQKLSESFGYEAILPFSMDAGIFLYQAMMVFLITIILGIYPLN
                     KIHRLRIADAMRN"
     gene            30982..32193
                     /locus_tag="VGA21_10640"
     CDS             30982..32193
                     /locus_tag="VGA21_10640"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX9651009.1"
                     /translation="MISKIAWRNVWRSPARSLVVIGAIVLGVWALVFTIGFVNSWVIK
                     FVENSVSQEYSNFQIHEVEFKKDSEMKFFIPEGDKLLVSLQEHQGVIAVTGRMKSNAM
                     ISSPKTATGGIIYGIEPSDEAEVTRLDSAIVDGYYFDGIRRNPILIGSKLAKKLKVKV
                     RSKVVVTLQDINGDITAASFQVAGIFEVMNPISNESKIYVRRSDLSRIVNLSEIHEIA
                     GLTNPSGDDEIIIPELKENYSGLQVEGWKDLAPELDLMLGQVKIQVGIILGIIMVALG
                     FGIVNSMLMAVLERFKEIGMLKAVGMKGRRVFYMIVMETIFMALVGGPIGCLLGYITI
                     EILGKYGLDLSRYSKGLAEFGIDVVLYPAVTPETYPLLTIGVVITALVATLYPARKAV
                     KLKTIDALHKI"
     gene            32195..32899
                     /locus_tag="VGA21_10645"
     CDS             32195..32899
                     /locus_tag="VGA21_10645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020526958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9651010.1"
                     /translation="MTIIKGENINKIYLETVVPVRALNGVDLSIEEGEFTAIVGPSGS
                     GKSTLLNIIGGLDSPTEGKITVAGIDISKLKESKLIDFRLHNIGFVFQSYNLIPVLTA
                     KENVEFIMLLQKWPKQKMEERAIQLLDAMGLSDKYHNKPIELSGGQQQRVAVARALAS
                     RPKFILADEPTANLDSKSTENLLGLMEKMNHEENATFIFSTHDQRVIEKAHRIITLVD
                     GRIASDELHTAGNHVR"
     gene            32889..34061
                     /locus_tag="VGA21_10650"
     CDS             32889..34061
                     /locus_tag="VGA21_10650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651011.1"
                     /translation="MSGRIILLSGLIFSGINTYGQEEETGIEHNWLYNGYIKDLLTVN
                     VLDDSTLVDNLIHNRLNFQWWPKEYLNIYVEVRNRIFFGDLVSAYPNYSELIDTNDDF
                     FDLSWTIVDSKDLVIHSMIDRLYVEYHKKDWEIRLGRQRINWGVNLVWNPNDLFNAFS
                     YFDFDYEERPGSDAIRIKRYTGFASSIEVASNFSNSFDELVSAAMWKINKGGYDFQLL
                     AGKSFQDIAIGLGWAGNIKGAGFKGEATLFEPYENTVYSTVILTTISLDYSFENSLYL
                     HASGMYNSQGLTEPGIGALEPILSRNRLTVRNLSPYKWSTFLQLSYPVHPLVTTGVAA
                     IFYPGDNALFLNPSIGISLPKNFDLDVLAQVFFNDTLFSDYKALAKLFFVRLKWSF"
     gene            complement(34058..36154)
                     /locus_tag="VGA21_10655"
     CDS             complement(34058..36154)
                     /locus_tag="VGA21_10655"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX9651012.1"
                     /translation="MSNSRKAEIYYEKLAYENAIELYERVLAKNPADLNAQTRIAECY
                     IKLNNPRGVVSWYRPIIENPSLMPIHKYNYAQALTALGNYEEAKTWYENYKQDVPDDP
                     RSDYKLKFLEDLDFYTRDSSLYLLTNLQVNSENSEFGAVEVDNELIFLSSRDQALFIK
                     YEDDDENEFSQAPFDIYISTFTDSSFSQPKKLHQVINTKFHEGPMTFYNDYSKVIFTR
                     SNYYSYRKGESEDGKIKLKLYSASLNPGGEWSNVKPFVYNDNEYSTAHPSLSADNLTL
                     YFTSDRPGGFGGNDIYFCQRIDNSWSEPVNAGGQVNTAGDEFFPFISEDGHLYFSSNG
                     HGGFGGLDIYRSTRFDGQFGNIENLGFPMNTSGDDFSFSIGKDGRKGFISSNRPGGKG
                     SDDIYQFEVRYKSIVGRVLESRTKEFVGNAEIMINDSARQTYTILKADESGYFQVDLP
                     LDTYFDLQAKKDNFGLEVPTRISTVVHSFQTDTADVFMWRHELFAQGRIFSNETQELL
                     PDVTVSLENLTQNTFTILETKEDGKYVFVLKPDQLYIIQAEKIDHVTSNFKLNTEGMI
                     ADTLLNDFVLEEVYVTKDVIFFDFNKAIIKPDAQPALNQMITNLKRHKDTYIVVSAHA
                     DAQGTFEYNLTLSNRRALAVVAYLKQKGIREDRITWRGFGEQLLINRCSDGVQCQEEE
                     HSKNRRAELKIEKSKN"
     gene            complement(36238..37146)
                     /locus_tag="VGA21_10660"
     CDS             complement(36238..37146)
                     /locus_tag="VGA21_10660"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HEX9651013.1"
                     /translation="MRQYLFLFFIFLGQVAYSQQQATFSQYMFNGLAINPAYAGSNGV
                     LTATALVRYQSVGLTGSPNTQSLAIHSPLVNKKIGIGLLLVRDNISVINQYSISAMYA
                     YRIKITEKGHLALGLQGGINSINAEYTRATIFNPNDPAFQQDVRSTRPNFGFGIYFNS
                     DRLTAGVSMPQLANNVFTRSPDLSTIKQDNPIILTVGYALDLSRAVKFRPSTLLKFVG
                     GEAVEYDLNAQFSFDDIIWVGASVRFLSAVDILAAVQVTRQLRFGYSYSITTNALNLV
                     DLGSHEVMISYQFKYPKDALISPRFF"
     gene            complement(37146..40916)
                     /locus_tag="VGA21_10665"
     CDS             complement(37146..40916)
                     /locus_tag="VGA21_10665"
                     /inference="COORDINATES: protein motif:HMM:NF037016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEX9651014.1"
                     /translation="MKLTRLILLSIMLLLFIENAFSQLRGQILKAGDGTIDSNGDGFV
                     SLTSAGFSIDGYYVDEFELKMFGIPIYEEGEVLNDIQAGALCGTTDLAVDTAGYSVYG
                     VLENNNLIFRFRLAGDNPSVEAYTILIDTDNKIGNDDPNATPENPGFEIDITFIKRSN
                     AGVYIYDIDGIEDCPAPIAFFPYSTHYQKAIAGTNSCDDPDYFYDFYIPFAALNTLFG
                     LTAQSELRFASVTNVSATCAMAGMISDIGGVNDADYTNNSDAFLDLTSNQCPTALEDL
                     CQTCAGFKSGKTPKPSINTPIKVGESIITGTAVKNADVFINRFDVSSVLVESITTSSG
                     TSGIWEATFATPLALGDSITARALLEGQCSSGISDSDLSFALVIVNTPPTINASTTAL
                     VYTENDPPINLFGDLSINDPDDFQMDEAWITFTVNYIQSEDIITLPPGQGLTLSFDPV
                     TGEVHITGVASLAVYQSVINGIQYSNSSENPSVATRTFTVKVFDGLDESNILTRDIQV
                     INVNDPPVLSGTAGQITYFEGDANVIVDNGILITDIDHTTLQGATVSISNNYIDTEDQ
                     LIFVDQNGITGNFDAVTGVLTLTGVSSIGNYQTALQSVEYSNSASIDPILLVNPLTRQ
                     ISFQVNDGIDLSNITSRGIDMASTNSPPVVVDDMGNPIDTLWVSTLEDIPIDVCLDVV
                     DPDGDAVDIISYALEFGQGTVDLDPLNRLCFTFTPAENYNGFEYIDIDVCDEATPSLC
                     DDIVVAIEIIPVNDEPVIVDPENNNPIDTLFFETNEDQPIDICLEAIDPENDPLELAD
                     AYSTGMNATIDIFDPVNLCFTYSPNQDYNGTDILEVVVCEVGSTTQCDTIIAVGTVLP
                     VNDPPQVTVGGVPVDTLYFDVLEDIVTKLCVDVDDPDGDFVDLVDASPIEGYGGAFEH
                     LPLGDLCFFFAGDTNETGTTFGEIVVCDAQVPALCDTAVVVINILPVNDPPQIISEDL
                     ELDTLRLATNEDIPINFCFRIQDPDPESEEEISINSQTSLGSYTEGTGPTECFLFTPN
                     ENIFGTEYATLSVCDNGTPTLCDLAIIEITINPVNDKPVAVNDTVVVQRNSSVSGNLT
                     DNDYDVESSVLIINTNPENAPLHGNVTLSPDGSFTYKPFPTYSGIDAFTYLVCDDDVE
                     PLCAGALVYIVVEDIPIIVYEAVSPNGDGMNDTWRVEGIELFPNNFIRIYDRYNNLVF
                     QTNSYNNQERAWFGQSNKGISNKSVPEGSYFYMINLGDGSKPLSGYLILKKE"
     gene            complement(41223..42461)
                     /gene="lysA"
                     /locus_tag="VGA21_10670"
     CDS             complement(41223..42461)
                     /gene="lysA"
                     /locus_tag="VGA21_10670"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002702591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEX9651015.1"
                     /translation="MELKNNVYTIQGLELKGLAEKYGTPLYVYDGEKISSQFKAIKSA
                     FSEHKVRIKYAAKALTNVSILKYIRQLGAELDVVSIQEAHIGLKAGFKPHEILYTPNC
                     VSFDEIQEAVELGLLVNVDSISILEQFGNAYHNSVPCCVRLNPHIFAGGNTKISTGHV
                     DSKFGISIYQLPHLVKIIKTHNLQVIGLHMHTGSDILDSEVFLKVAEILFGIARDFGD
                     LKFIDFGSGFKVKYREGDNTTNIFDLGKNLGTAFKQFCSEYGRELEIWFEPGKYLVSE
                     AGMLIARTNVVKTTPATVFAGVDSGMNHLIRPMMYEAYHDIVNISNPTGINRIYTVVG
                     YICETDTFGMDRKLHEVREGDLLLIKNAGAYGFSMSSNYNSRLRPPEILIAGGKDYLI
                     RKRETLADVTRTMVEVDVDA"
     gene            42620..44122
                     /locus_tag="VGA21_10675"
     CDS             42620..44122
                     /locus_tag="VGA21_10675"
                     /inference="COORDINATES: protein motif:HMM:NF025854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX9651016.1"
                     /translation="MKRKTIISIIILMSLALAGLVSFQLYWINSVIKLNNERFISNVH
                     ESLNMVISRLENTENLFFVSRGVSRVLFETEDDKSKFTDSLANFKVMIHADTTKPGEI
                     AKKEIASLKAKIQNDSTARQRVQKELENGWPPDRGIVRKSGMVQIILQELASPPKVIQ
                     HRINKEVLDSLLHQEFANKGIHLKFEYGVLNPVSDEIILANTKNVDHLRETDLKATLF
                     PNDIISNANYLTVNFPDKGSFLFKQIWATLASSLVLLLVIVGCFAFALLTIIRQKKLS
                     EMKNDFISNMTHEFKTPIATVSLACQALGEPAILNQPNIFKKYLDIIRDENERLEDQV
                     ERVLHLASMEKEDLNLKFDKVNLNEILKDVFQQIYIQVEQKKGSIVLEEKSPEIWVNG
                     DANHLFSVFLNLVDNANKYSQDKPDIKIICEKKGDKVMVEVMDKGIGMSKESIRKIFD
                     KFYRVPTGNVHNVKGFGLGLNYVKNIVEAHGGRVSVHSETGKGSSFKVEL"
     gene            44119..44817
                     /locus_tag="VGA21_10680"
     CDS             44119..44817
                     /locus_tag="VGA21_10680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020530981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX9651017.1"
                     /translation="MNERKKLLVVEDDPNLGQILREYLEIKGYDAELCTDGEKGLATY
                     RKGKFDLCILDVMLPKKDGFSLAREIRIMDENTPIIFLTAKNLKQDTIEGFKIGGDDY
                     ITKPFSMEELILRLNAILKRTTPNLNGSGNQKFTFGSFEFDYGQYVLTCKGKSWRLTA
                     KEADLLMLLCQNINKLLDRKLALKLIWHDDSYFNSRSMDVYITKLRKYLKEDEQHVRI
                     ITVHGQGFKLVSIN"
     gene            44968..45717
                     /locus_tag="VGA21_10685"
     CDS             44968..45717
                     /locus_tag="VGA21_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015264708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]
                     imidazole-4-carboxamide isomerase"
                     /protein_id="HEX9651018.1"
                     /translation="MIQIIPSIAILNGKVVRLIQGNYFAETSYKESPLDVAKKYEAHG
                     IEVVHLVDLDGAQRGSPVNYHILEAIAGHTSLKVDFTGGIHTDGDINKAYEYGATFIT
                     AASIAATNPNLFGSWIISYGRERITLGADSLDRKIAIRGWQKNTTIDLFEHIDYFYSR
                     GLKYVKTTDISRDGVMQGPNFKLYRQLMKTFPGMSILASGGVKNMDDVDKLNEIGIFA
                     VIIGKAFYEGRIKLKELEKYLVTHPIQTLKS"
     gene            complement(45731..46090)
                     /locus_tag="VGA21_10690"
     CDS             complement(45731..46090)
                     /locus_tag="VGA21_10690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651019.1"
                     /translation="MKNPLLIVSFITCLLIGTNSFATSLKEDKNNKEGNQESQQTGEK
                     SDLAEVDSAQIDLDSEIPEQPKGLLNKQIKPEEKRPTGELSVPPEDQNNSPVSSFNFL
                     FYLIEKFKFPDLLDESF"
     gene            46195..46533
                     /locus_tag="VGA21_10695"
     CDS             46195..46533
                     /locus_tag="VGA21_10695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010608281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HEX9651020.1"
                     /translation="MALISWEDFESVDLRVGTIISASEFPEAKKPAFKLEVDFGAAIG
                     VKKSSAQITKHYNPQILLGKQVLCVVNFPPRQIGPVISEVLVTGFPDETGEVILAIPD
                     KEVPNGKKLF"
     gene            46590..47408
                     /locus_tag="VGA21_10700"
     CDS             46590..47408
                     /locus_tag="VGA21_10700"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX9651021.1"
                     /translation="MHEAFLKAFSDYEIPFNLSLAEFSKKFVEKLSIDFGLSPAIFHE
                     GEIAAFIFTSINYYDGRLTAYNGGTGVLPAYRGRRYVQQLYNLLLPKLQNRMVYHCVL
                     EVLTRNERAIKVYESCGFGKSRYYHCFKLLGRKRFKNYHYPWNIRISTTPDWKTYTRY
                     FDFLPSFLDTPAMIDQNLKNEKIIEAIIHGEVAGYAIYQKAQGRISHIGVASNWRGKG
                     IGSSLIDYIYEDAANKNLTIININKEAVGVKDFLTRMGFENQFDQWEMVKSVDK"
     gene            47405..49849
                     /locus_tag="VGA21_10705"
     CDS             47405..49849
                     /locus_tag="VGA21_10705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018479972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     UDP-N-acetylmuramoyl-tripeptide:D-alanyl-D-alanine
                     ligase/alanine racemase"
                     /protein_id="HEX9651022.1"
                     /translation="MITFNSLPGITNGKIVNQLKDYEIRYLAIDSRKIISSPHTLFFA
                     IKGDRHDGHNYLESLVKAGITNFVVETDLPVSMDISSCNILKVKSSTEALQHIAANHR
                     AEFTLPVIGITGSSGKTIVKEWLFQLLQGERIVFRSPKSYNSQVGVPLSVWQMNAHHE
                     LAIIEAGISKPGEMEKLERIIKPEIGLFTNIGTAHDSGFASREEKAREKTRLFKNCKL
                     IIFCRDDLQVENAIKELGRKKTFTWSATDKDADLQIVKTTSRELVCRFNEIEFTIEIP
                     GDNKASIDNSSHCIAALLSMGIPVKKIKESFKNLQPVSMRLELKKGINNCPIIDDSYN
                     NDLMGLEIALDFLTHQSKKGKKTVILSDILTSGIPDEELYQLVDKLLDQKKISRLIGV
                     GPGINSMNEKFTLPRKFFETTEELLKELPRISFENETILVKGARDFKLEKVVDKLVLK
                     IHSTVLEINLDALTHNLNYYKSKLKPAVEIMAMVKAFAYGSGSVEIARWLQFHQVNYL
                     AVAYADEGLILRSHGVEIPIMVMNPTPDSFDKLISYNLEPEIYSEKILKSLLNYLSEK
                     AEKVSIHLKTDTGMHRLGFDEEELNHVKELLQNEKITVESVFTHLAAQENADQDEFTR
                     IQIQSFEKMYTQISGWIETKPKRHVLNSAGILRFPEFQYERVRLGIGLYGIDPIEAGN
                     DLMPVGTFRTLISQVKKIKPGETIGYGRIGKAEQGLKIATIPVGYADGFNRRFSNGVG
                     QVIINNKRAKVIGNVCMDMTMIDITDIDASEGDEVIIYGAENPIDEMAKKIGTISYEL
                     LTGIGERVKRVFVQET"
     gene            50425..51345
                     /locus_tag="VGA21_10710"
     CDS             50425..51345
                     /locus_tag="VGA21_10710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEX9651023.1"
                     /translation="MKSYSRIAIFFIFMAFILVAGKSQAQNNNTVSMNSSEYAIRITI
                     INGATMSPVRNATITVDSLTRTYKSQVEGVYSIRLPRGNHTLKIVASGYQDVYQPVSV
                     FDDGQFKLKMYRLIERKDRLNVGRYPVKLSFDNIQKRKGLMFGFGLGTGYSNHAASYT
                     VRSDGFGNFKELSGYRLMTLNFEIGYGLSPKFFINYNYKYSPPNTTVSAYQSTYHGGE
                     LNFSPGRWGRGLITLGGGYQIAKDKEGTLADGYLVNAGLGYEVSPHFVFEMTNFLGTM
                     NNKVDEKTGNRFMNTSTEFILQISINYLFY"
     gene            complement(51416..51913)
                     /locus_tag="VGA21_10715"
     CDS             complement(51416..51913)
                     /locus_tag="VGA21_10715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651024.1"
                     /translation="MHQFFKRRAVSFSASAWFVVATLIFTAYIYYNNLTSLPAQIFFG
                     ILIIITFLLFVLQRVVVVDLDQITLRKSLLLFSVGIGIAAPVPLHHIKEVFINKVKFS
                     QKRTAPTHSSQTLTSQGFEFKAFLLLKDGSTYLLDSSPNHEEILDELISLAEVLNIEL
                     VDNSG"
     gene            52131..53066
                     /locus_tag="VGA21_10720"
     CDS             52131..53066
                     /locus_tag="VGA21_10720"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX9651025.1"
                     /translation="MKKDEIKESNFSEEHIYISVETELKLRHSELNKGVRHCEIINSE
                     NKAGKATVRVGKHFIVYLFVNGDADLISSIDQKVTHISNDHYAMMVFPVSDWSFEIEL
                     QPEQNLKLLCISISELHVIFGNLDLQDPQMLKDALKNYRQKSFLTLKPSTPAVRTIIH
                     QLFTIRGEGISRMVYQKGKIMEFLSVFLGSRDNGISEAECPYITDSLDWEKIRNAEKI
                     IKTDLAHPPTIKELAKKVGTNEFKLKIGFKHLYKNTIYGYLNDYRMDYAKEMLSNKNV
                     LIKDISEKVGYSSTSHFIASFRKKFGITPKQYLRG"
     gene            complement(53072..53740)
                     /locus_tag="VGA21_10725"
     CDS             complement(53072..53740)
                     /locus_tag="VGA21_10725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651026.1"
                     /translation="MRKQICLVSLFLFIPILAFSRQTASSGSSYFYSNSTQSLSELPY
                     NFEFDKYYLVKPTASVGGTRAFAIIGFVLPAPLIGLTTAAGLSDDFTSGTILGASALL
                     LGGVGIPLVAAAGSRMRKESGSRSSLGIIITGWALYGIAMANGVVAVGLSASEIEVPL
                     PVGLTIAILGSASSILFGVEAMNAANDASGISAFRIQPLIGFDTTLTGKLYQNIGVRI
                     MLTK"
     gene            complement(53896..54204)
                     /locus_tag="VGA21_10730"
     CDS             complement(53896..54204)
                     /locus_tag="VGA21_10730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651027.1"
                     /translation="MYDKVGTNATGFEVVSVPVNRDAEKIYSGISAQISVICRREKFF
                     SVNPRADLLKKISLIPLISRITAEKIYSGISAQISVDLREKRVFLRKSAPICRRVFII
                     "
     gene            54344..>54979
                     /locus_tag="VGA21_10735"
     CDS             54344..>54979
                     /locus_tag="VGA21_10735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651028.1"
                     /translation="MRRQTIFFISLFILPVTLPAQNNHDVGEPGQPIENIILPYGRVG
                     IGTLTPLYKLHVAGNLFVSDTTWANNLITDRLTSEFLTISKDATVNRDLFVNGKVGIG
                     TLSPQYKLDLTGSLRASETLFANKLITSFAESGDLTISNQATLHKAFIQNTLGIGVES
                     PAYPLDVLGDIHASSALRSNSVETSQLMVNSGAAFSGNVIMNNYLGIGKSNP"
BASE COUNT        17563 a        10177 c        11406 g        15833 t
ORIGIN      
        1 ggctaaaaaa tgatatttat atttatccgg gagacttaat ctatattgaa ccggtcaaaa
       61 gaccattcag tgaaggcctt agagattcgc ttccgttaat ttcaacatta acaagcataa
      121 ttaccctatt cgcactggtt atcacaatac tataacttat gatccaccag gaagaaaaat
      181 tactttatga caaatcgcag gagattgact ggctgaagct gcgaatgatt ttgcaaaaat
      241 ccttaatatg gatgttgcta atcattttca ccacaaattc aacggcgtat ttataccttc
      301 gatggaccaa gccggttttt gtatccgaat ccaatttgaa attggatatt aaaagtgaag
      361 cgggaatttt ggggtttgat gccatagatg aggaacaatc actcggtact atttctggcg
      421 agattgagct catcaaatca agaatgtttt cagaaagggt aattaagagt cttgacctgg
      481 gggtaagcta ttatagtgtg gggaaaatcc tgaacgatga gcgatacaaa ttttcgccat
      541 tcaggattaa tgtaattaac atggatccaa atctatttga cacacccgta tatgtagaat
      601 ttcaggaaaa cggtgatttc tcagtctcat tatccattga atcattggcg gataagatgg
      661 tcttttcttc tattgatact attagagtat caaatcttga attcacgttg aacataatca
      721 agcctctcaa cagagaaacc ttagaaacta aattcttttt tcttgtcaac tcataccaaa
      781 aactgattac ataccttgaa actaatctgt ttgttgagcc ggtgaatctc agtgctaaaa
      841 caattaaagt tagctttaga gatcataatc cttacaaggc gatggatttg gttaattcaa
      901 ttgataccct ttatttgaat tattcaaaag aggaaaaaca aagagcgaat tttcagaaaa
      961 taaagtttgt tgatcagcag ttattggcaa ctgatgagaa gctgtctcaa attgaagatt
     1021 accttgagtc ggaaactctg ggattgcaag gatttagtct tgataatgag tcgaacgaag
     1081 ttattagcag gctgaaagaa gtaaagtcag aacttattgc gattcataaa caacatcagg
     1141 aaagttacag tttggtggca ggcctggata atggagattc agtctggatc ccgaaattca
     1201 tggctattca tccggaaata attcaggaaa taacagattt taaaaacagc aaactaaagc
     1261 ttagcaaatt gcggaagact tttagtgaaa atacccgaat agttttggag gaaaaggagt
     1321 tggcagaaat gcgaaaaaga aatttggttt tgtcattgga taaattaatt ggaagtcagg
     1381 aaattttact tggcgaattg accatggagc ggaactctct gagcaaaaag ctcacatcaa
     1441 taccccaaag gaaaaatgac ttcaacaaaa caaacaggta ttacaccatg tatgaagatt
     1501 tttacctgag ccttatgcaa aacaaaactc aattccaaat tgctcaggct ggcataacca
     1561 cagactttaa aatattatct caagcttcca tacctgccag cccggtttct ccaaggaaga
     1621 caattatctt tggaattggg ttcatgtcgg gattcctgat ttgctttttc tatataggga
     1681 ttcgctatgt gttgcataac aggattacat cggtagaaca aattgaaagc aaaaccaaat
     1741 tgccagttct tggttccata cctttctatc attcagaaaa attgaaagcc agcaaacttc
     1801 ttgttggcaa acacccccgg tctgccatta gtgaatccct cagaggtata agaactaatc
     1861 ttgaattctt cctgccatca aaaaagaata agatccttgc ggtaacatca acagttagcg
     1921 gagagggtaa gactttcttt tcagttaatc ttgccggcat tatctcactt tcatcaaaaa
     1981 aagttattgt ggtagacctt gatatgcgca ggccaagagt tcaccaggca tttggaatag
     2041 aatgcccaaa ggaaggggtt agctcaattc ttatcggaaa gaatagcctg gaggaatgca
     2101 tacacataac ttcgatgcac aaattgcatt ttctttccgc ggggcctcca ccacctaatc
     2161 cctcagaact gctgctgaaa cctgaatttg atgaattgct taaaaaattg tcaatgctat
     2221 atgatgtaat tattctggat acacccccgg ttggattggt aacagacgga atactggctc
     2281 tcaaaaaggc ggatctgcca atatatgttc tcaggtccga cttctccaaa actcactacc
     2341 ttgaaactct aatgagactt aacaaaattc acaagtttag caatttaacc gtacttttga
     2401 attcaataaa aatgtcctcg cagggatatg ggtatggata cggggaagga tattaaatgg
     2461 gtgaaaatac tccttcctga aaaaaaactg attttcaatg aatcttttca gcagaagtaa
     2521 acctgaaaaa atacttgagg ttgacatgca ttcgcatctt ataccaaaaa tagatgatgg
     2581 atcgcagtcg acgcgagaaa cgattgaaat gctgaaattc atgcacgaaa tgggcataag
     2641 aaaagttatc actactcccc atattatctc cgacctgtat cctaatactc ccgcaaaaat
     2701 tatggattct tttagtcttc tgaaccagga aattgccggg gaaaatattc cgattgaatt
     2761 ggaagtaagt gccgaacact atctcgacga attcttaatg caatcccttg accggaatga
     2821 catacttccg ttcggaggga aatatttact ttttgaaaca tcttttatgt taaaacctgc
     2881 cgtactggaa gatttcattt tcagggtaat ttcgaaggga ttaaaaccgg ttatggctca
     2941 ccctgagcga tatgtttatt ttcaggatag ctgggatctt ctaactgaaa tctggaatag
     3001 gggcgcactg tttcagataa atatttcatc tttgggtgga ttttattcca ggccggcgaa
     3061 agttgttgcc caaaaattaa ttgactccgg gcgggttcac tttttgggaa cagatgccca
     3121 caatatggaa catctaaaaa aatgtgccat agtaatgaaa aataagtatg tccaaaaagc
     3181 tttgcatctg ccattgttaa acttcgaact ctcgtgattt taataaccgg cgccaatggt
     3241 ctggttggaa gttttatttg ccggaagtat ctggatgccg gctttgaagt ttccatcctg
     3301 gtaagacctt caagtgattt aacctttctt aaagatatcg aggggagaat aaaaatccat
     3361 tatggagata tactagatgt gctctctctt gagcaggcaa tgcaaggttg caaaacagta
     3421 gtacacagcg ctgcattagt ttctcttctg ggtaaggaga gaaaaaaaat gtttaaagtg
     3481 aatattgaag gcacaaaaaa cgttgtgaac acctgtttga aaatgggtgt tgaagaactt
     3541 attcatatca gctcaattgc agccctgggg agaatcaaag aagttataca gatcgatgag
     3601 aacgcgaagt ggactcaatc gaaattaaat tctggttatg ctgaaacaaa atacctttca
     3661 gaactggaag tttggcgtgg tatggaagaa ggcctaaaca ttataattgt taatccctcg
     3721 gtggtactgg gcattgggaa ttggaataaa tcaagcggcc agatattcaa atatgtacac
     3781 gatggaaatg ctttctatac aaaagggaaa gttaattatg tggatgtcag ggatattgcg
     3841 gacgcggttt ttattttgca ccaaaaccgg atttatggtg aaagatttat actaaacgcg
     3901 ggtgtttcaa cctataaaga tttgttcaac cttattgcca aagaattagg aaaaaaagct
     3961 ccgttatttc gcgtaggcac aattttgctc tggatggcaa tggtatttga tcagctgaag
     4021 tcattgttca cccgaaaatc tccattaatt acccgggaaa cggtaattct tagtgaaatg
     4081 aacttctttt ttgataataa gaaaattatc gaaaatacag gaatagattt ccgacctttg
     4141 caggaaactg tgaggtgggt atgtggtagt cagactccat aatatccgcc aaagggtttt
     4201 ggtatcatta attaattgct aaatttcata aaccaaccga agggtcttaa tgtcagaaag
     4261 ttttaagagg aggcgtgagg ataacgagct aatacgaaaa tttgaagacc atctgaaaaa
     4321 tgacagaagc ttctattttg atgttgattc ttacgaagaa ataattgatt attacctcga
     4381 gcggggcaag tataattatg cgctgagggc ctgcacgctg gccattgaac agtacccata
     4441 ttcaaccgac attttattga gcaaagcaga aattctagcc aacctggaag attatgatga
     4501 ggcattaaaa gccattgaca atgtattaat atttcaaccc accgattccg aagttctttc
     4561 actcaaagcc aacatactca tgcaagctgg ttattatgaa agagctgtgg aggctttcag
     4621 agaaaccata atctatggtg accaaagcgg tgaactttat tatggacttg gtcttgcatt
     4681 tcaaagtctt gaaaaatatt ccgaggcgat tgattgctac aagaaagcca tcgacataaa
     4741 tcctaaacat gaaaattcac tttacgagtt agcctattgc cttgatgtta aaggtgagct
     4801 ggaaagcagt gttgcttact acaaacaatt tattgagagt gatccatact cgcatttcgc
     4861 ctggtataac ctgggaattt tgttcgagaa aatggaaaat tatgaagaag caatcaaggc
     4921 ctatgaattt gctacagcga tagacgagag ttttgcctca gctcattata acatgggaaa
     4981 tacgtacatg caattgagta aatattcaaa agcactggat gcttacaaga aaactttcga
     5041 taaagaagga gccagtgcag acatctattt cagcattgcc actgcctatg aaaaactgga
     5101 tcagtttgag ttgggcctta agtactatcg aaaagcggtt aaactggata ttttctatga
     5161 tgatgcctgg tttggcgcag gacgctgcct ggaaaaccag ggcaaatttt atgaagcaat
     5221 tcatttctat aataaagcat tgaagctgga taatgaaaat cccgaatact ggaaatctgt
     5281 tgccgacatg gaattcaaag tgggaaatct ggtctcaagt ttaactgctt atgaggaggc
     5341 cagcacccgg gatcctgaaa atatggcagt ttggctggac tggtcctttg tttattatga
     5401 acaaggtgat tacgaaaagg caatgagttt ggtaaaagaa ggcatctcat taatccctga
     5461 aagctccgat ctttattacc gggcgactgc ctatttggtt tccggaggta aatatcagga
     5521 agcctttgct tatttggaaa atgcattaat tttgaacttc gataatcata cggttctttt
     5581 tgaattcttt ccaaagttgg aaactcaaaa agcgcttttc agaatcattg atcagtttag
     5641 aaaagagaac tcctaaatga atttgaatta cttccttaaa gatttacctg aaagaactca
     5701 aaaacccagg cagcatggat tcaccatggc aatggataaa ggtctgagtg tccgtgaagc
     5761 tgaagatttc ttaagtgttt gcagcgaatt tgtcgatata gtgaaattcg gttgggcaac
     5821 aacttacgta accccaaacc tcatcgacaa gattaaagtg tacaaagcgg ccggtgtgcc
     5881 ggtttatttt ggaggaacat tatttgaagc cttcatagtc agagatcaat ttgatgacta
     5941 tagaaagatt ctggataaat atgagatgga gttttgtgag gtttctgatg gctcaataga
     6001 actggatcat gaaaagaaat gtgaatacat tgctaaactc aaagagcagg tgaccgtact
     6061 ctctgaggta gggtcgaagg atgcggagaa aataattcca ccgtatttat ggataaaact
     6121 aatgcaggag gaactggatg ccggagcatg gaaggtaatc ggtgaagcaa gggaaggcgg
     6181 caatgttggt ttgttcaggt caacagggga agtaagatcg ggattagtag aagagattct
     6241 cactaaaata ccatttgaaa aaataatttg ggaagcaccc caaaaatctc agcaagtatg
     6301 gttcattaag ttgctcggcg ccaatgtaaa tctgggcaat attcaacctt cggaaatact
     6361 tcccctggaa acaataagac tgggtctgcg gggagacacc ttcaatcttt ttttgaataa
     6421 gaataactcc aggtaaagtt catgtcctgg ctaaaaaagt atcctgtaat atacctgaaa
     6481 ggaattgcca tgggaattgc cgacgccatc ccgggagtta gcgggggaac tatagcacta
     6541 atcgcgggaa tttatgaaca attaattgat tccattaaag catttgacat tacggcaatt
     6601 aaatatctcc ggaaatttca aatcagagac ttttggagat atgttaatgg aaatttcctt
     6661 ctcacgctcg tattgggaat agcgaccagc ctggtaacgc tttcacattt tgtcaccttt
     6721 ttattacgag aatatccaat ccagatatgg tcatttttct ttggattgat aatcatttca
     6781 tctgcagtag tcttaagaag gattaacaaa tggaatccag gggttatcat aagtttatta
     6841 atcggtgtgg ctgccgcatt cttaattacg aaggcaactc cggcgcaaac acccaatgaa
     6901 ctctggttca ttttcataag cggttcaatt gcaattattg ctatgatact tccagggatt
     6961 tccggagcat tcttgttgtt gctgctggga aagtatgaat atgtatacct ggcattacga
     7021 gatttgaatt tggcaatcat cggggtattt atagcgggag caattaccgg attactcagc
     7081 tttagcagag ctatttcctg gctcctcaag aattatcata attctgccat cgcgttatta
     7141 agcggcttta tgataggatc tctgaataaa atttggccat ggaaaataat tacccttttc
     7201 agagaaaatt ccgaaggtga gcaaattcca cttctcgaaa aaaatgtgct ccccgcacaa
     7261 tattttcagg aaaccggaaa tgatcccttt atattccagg caattctttt tgttgccgtg
     7321 ggaatcttta ttgtagtttt gcttgagaaa ataagcatct caagaaacaa atctacgtga
     7381 aattactagg gttaatcggg catcctttaa cgcattcttt ttctgctgat tatttcaata
     7441 agaaatttga gaggttaaaa ctaactgact ttaactatgc gctctttgac tttattgaaa
     7501 tttcggatat aaggcgctgg gctgcaactc atcctgattt acttggattg aatgttacca
     7561 ttccatttaa ggaaaaaata attccttatt tggatcaggt tgataaaaat gctaagaaaa
     7621 ttggcgctat taatactata aggatttaca acgacacctg gaaaggatac aatacggatt
     7681 atattggatt cagggaatct ttaaaaaaaa taatccgatc agagtcgaaa ctcaatgccc
     7741 taatccttgg ctgtggagga tcagcgaaga cagtaagcaa agtattgctt gacatggaaa
     7801 tccagtttca aatgatctca agaaaggaat ctaaagacgc tctttcctat gaccagctga
     7861 actccggcaa ttttatttct gattccaggt taatcattaa cacaactcct ttggggatgc
     7921 atcctgatat taattcctta cctgctattg aatacaatca aatttctgat aaacacatct
     7981 tgtacgatct cgtttataat cctgagaaaa cggcctttct ccttgaaggc gaaaaaagag
     8041 gagcgcggat taaaaatggc cttgaaatgc tttatattca ggctgaacaa tcctggaaaa
     8101 tctggacaaa taatttaaag tgaattttca gttaacaagt gaatttgaac caacaggtga
     8161 tcaaccggag gctattcaag gccttgttaa cggactcact tcgggtgaaa attttcaaac
     8221 tctgctgggt gtaacaggtt ctggtaaaac attcactata gccaacgtta ttcaaaaact
     8281 tcaaaaacct acattggttt tatgtcataa taaaaccctt gcggcccaat tatacggaga
     8341 gttcagacag tttttcccaa ataatgccgt tgagtatttc atatcttatt acgactatta
     8401 tcaacctgaa gcctatatgc cctcaacgaa catttacata gaaaaggatc tgtccatcaa
     8461 tgaggagatt gaaaaacttc gccttagtgc aacgtcatca ttgcttaccg gcagacgcga
     8521 tataattgtg gttgcttcag tctcctgcat ttacgggatt ggaaatcctg atgaatttga
     8581 aaagaatgtt atttcattaa ctgttggtga aagaattcct gtaagcaaat tgttgtataa
     8641 cctggttgat attctttata gccggtcgga ccttgacttc aaacgtggaa ccttccgggt
     8701 taaaggagat actgtggata tttttgttgc ctatgcggat tttgcctttc gcatattttt
     8761 ctggggcgat gaaatcgaaa atattcaggt tattgacccg gcaacgggcg aaaaagttgc
     8821 ggaggaaaaa tcggtttcaa tttttccagc caatttattt gtcacaggcc gggatgtaat
     8881 tgatgtagcg ataaaagaaa ttcaggagga tttagtgatg cagctaaatt tatttgaaga
     8941 agaaggcaaa gttggtgagg ctaagcggct gaaggataga actgaatttg acctggaaat
     9001 gattcgggag ttgggatatt gcagtggcgt tgagaattac agcaggtatt ttgatcgaag
     9061 aaaagccgga gccagacctt tttgtctaat tgattatttt cccgatgatt ttcttttagt
     9121 agttgatgaa agccatgcga ctatcccaca ggtaagagcc atgtggggtg gtgaccgctc
     9181 caggaaagtg aaccttgtgg attatggatt cagattacca tctgcgctgg ataacagacc
     9241 gctgactttt aatgaatttg aagcaatgct gaatcaggtg gtctttgtca gtgcaacccc
     9301 atcggattat gagttaagaa aatcggaagg tgtaattgtt gaacagataa taagaccaac
     9361 cggattgctt gatcctcaaa tcgaagtcag accaagcgcg aatcaaatcg atgatttact
     9421 ggaggaaatc gatgaaagag ttaaagtgag tgaaagaata ctggttacca cgcttaccaa
     9481 aagaatggct gaggaattga gcaaatatct ggacaaggca agtgtaaaat cccggtacat
     9541 ccatgcggag gttatgccgc tggatagagt agaaataatt cgtgaactga gacttggtat
     9601 ctttgacgta ctggtcggcg ttaatttatt aagggaaggc cttgatttgc ctgaagtgtc
     9661 actggttgct attttagacg cggataaaga aggatttctg agaaatgtaa gaagcctggt
     9721 tcaaaccatt ggacgcgccg cgcgtaatga aaggggcaag gtgataatgt acgcggatgt
     9781 tatgacagat tctatgcgcg tagccattga tgaaacaaac aggaggagaa atattcagca
     9841 agaatacaac cggatgcatg gcataacccc ggtgactata aaaagatccc gggaatctat
     9901 tatgggccaa acaagagttg ctgattcatt aaaaaacaaa aagaaattct atgttgaacc
     9961 ggaaagcaaa tcagtggctg ctgacccgat tattaaatac ctggacgaat ctaaattaga
    10021 gaccttaatc aaagataccc ggaagaaaat ggaattagca gcgaaggatc ttgattttct
    10081 tgaagccgcc cggtacagag atgaattata tgaactggaa gatttcaaag agaagaaaaa
    10141 gaataaaagt catgaaaaat aaattatttc tcatcgtatt catactaacc gcgcaatggt
    10201 gtgttgcaca aagttcaaaa tcctttgtaa tatctgtagg cggcgatgta attaaaaccg
    10261 acattcagaa ctttgcagat aaggttcaga ccggagcgga actcaattat tacctgcttc
    10321 gcaattttac agttactgca ggttttgaat tttggagttc cgggagagaa agcgcggttt
    10381 tgggaatgcg atattatccg caccggaata tttttgccag attcagagga ctgataggtg
    10441 ctaaccaggt agccttaggg gccgggtact cttacccgct gaatgagcgc ttcaaacttg
    10501 atggtttggg tgatttttat tttgattctg cagaatttgc tttcagggta ggcttctctt
    10561 atgttatccg gtaaatcttt attagtagga ttctgaaaat caaaatactt ttggtttcaa
    10621 ataaaaaaag cctccggtaa aggaggcatt taatttattt tgcttcgctg tacctccggc
    10681 ttacttcttc ccaattcacg atattccaga aagaagcaat atactccggc cttctgtttt
    10741 ggtatttgag atagtaggca tgttcccaga catcaagtcc caaaattggt gtgcaaggat
    10801 cagtcgccac atccatcaac gggttgtcct gattgggagt tgagcaaaca cataatccac
    10861 cgttggcttt tataccaagc caggcccagc ctgagccaaa tcgtgtcgct cctgcattgc
    10921 tgaattgctc tttaaacttg tcgaaacttt caaaagcctt acttatagca tcactgagtt
    10981 caccggaagg tgttccgcct ccatttcctg aaagaaccgt ccagaataaa gaatggttat
    11041 aatgcccgcc gccattattt ctgatcgccg gcgacaatcc gctaaccttg ttcattaatt
    11101 cttcaagtga cttgccctca ttgtctgttc cggcaattgc ttcattcaat ttagtgacat
    11161 aagcactatg atgcttggtg tgatggatct ccatggtttg cgcgtcaatg tgaggctcca
    11221 gagcatcgta tgagtatggt aattttggta attcgaaagc cataattata ttcatttatg
    11281 tttaacaatt cttataatgc gaatatacta atttcttatt tgccggaaaa aggcatcaat
    11341 aagtgatttg cactcttcgc ctaatactcc ccctttaatt ttggtcttgg gatgaagaat
    11401 tttgctgctg gtttttctat accctttttg ttcatctgtt gccccatata ctaacctccc
    11461 taactgcgac caaaaaattg caccgccgca cattacgcaa ggttccagag ttacatatag
    11521 cgttgcttcc ttgaggtact tagcgccaat gtgattgaac gctgatgtta atgcaatcat
    11581 ctcagcatga gctgttgaat cattcaattg ttctgtctgg ttataagctt ttgcaatcac
    11641 tttcccatcc attaccacga cagcacctac cggaatttcc tttttagtga aagcttcttt
    11701 agccaacttg agtgcttcct tcatgaaaaa ctcatcagtc ttagggtgca cggtcatcct
    11761 acttaattct gatactttcc ataatttgat ttgcagtatc ctcaaagtct ttttgaagat
    11821 tctgagggca agtgaagttg aatagtatag tacgaccctg gtcaagggta tatcgaagat
    11881 tagtatactt tcttatcggg cgcagatttt cagccttctc ttctttgctt acactgtaaa
    11941 actccagcac aacaaactct cttttgttaa tggtttgaat ttcttccgta atcatagtta
    12001 tctcggaata tatttcatac aaactggctt tgtaaaaccg catcagcagt tccaggtctt
    12061 gcgtattcca aagactgaat gaaaaattca caacaaattc agccgtgcgc gatggatctg
    12121 tataaatggc aaccggtctc cggtaggatt gagacctctg ctctaaatcc tgggaagaca
    12181 tttctgcata actttcagga atctttacag taataccttc tgaaacttct actttcacaa
    12241 acttttctcc cgggaacgga gtgaataaca ggaaaatgaa aaggcttaac ttcattagac
    12301 ttattctgga caaaattaac actgattggg gttaaaaaaa actgaaaaac agtgacatga
    12361 ttaaacaatt tataactttg ccacacttta agtaggcgaa atgtacagaa cacatacttg
    12421 cggagaatta cgagtgtcac ataaggggat aaatacaact ttagccggct gggtacagaa
    12481 gattagagat aaagggggaa tgatttggat tgatctaagg gatcgctatg ggattactca
    12541 gctaattctg gaagagggta agtcagacaa taaacttatt gaacaggcaa gagaattggg
    12601 gcgtgaagat gtaattaaaa tagcgggaat tgttcaggaa cgttattcaa aaaaccaaaa
    12661 cctggctaca ggagagatag aggttttatt atcacaactg gaaatgctaa atgagtcaaa
    12721 tcttccacct ttcattatag aagatgaaac agatggtgga gaggatttaa gaatgaagta
    12781 ccggtacctg gatcttcgcc gcgggcccat gaaagaaaac ttgcgcttga ggcaccgttt
    12841 aatgaattac acgcgagatt atctcacaag aaatcaattc attgaagtag agacaccggt
    12901 tcttattaaa agcacccccg aaggtgccag ggattttatc gttccctcgc ggattaacca
    12961 gggggaattc tatgcattgc cccagtctcc ccaaactttc aagcaattgt taatggtagg
    13021 cggtcttgat aaatattttc aaattgtaaa atgttttaga gacgaagatc ttagagcaga
    13081 ccgtcaacct gaattcactc aaattgattg tgaaatgtcg tttgtgcacc gggatgatgt
    13141 gctaagcatg ttcgaaggtc ttatcaagtt cttgtttaag gaagaaaaag gagtagacct
    13201 tgctgatttt gggcgaatga cctacgctga cgctatagct aattatggat cagataaacc
    13261 ggacttgaga tttgacatgt catttattga gttgaattcg ctggtaaaag gaaaagggtt
    13321 taaagttttc gatgatgctg aattggtggt gggtatcctg gcaaaaggat gcgctgaata
    13381 cagcaggaaa caattggatg aactaactga cttcgtaaaa aaaccccagg tcggagcaaa
    13441 agggctggtt tatgtcaaat ttcagcaaga ttcaaccatt aaatcatctg tagacaaatt
    13501 ctacaatgaa ttggatttgc aaaactggaa aaaaaattgc tctgccggga acggtgattt
    13561 gttacttatt atcagcgggg atgcaaaaac cgcaagaact gccttgtcaa ccttacggct
    13621 tgaaatgggg aatcgtctca atctcagaga ttcgaacacc tacaagccat tgtgggtgct
    13681 tgattttcct ttgctggaat gggaagaaga atcgggcagg tattttgcca tgcatcaccc
    13741 gtttacatca cccaaaccgg aagaccttgc aaaactggaa tctgatccag gctctgttaa
    13801 tgcgaatgct tatgatttag tcataaatgg agtcgaaatt ggaggtgggt ctatcagaat
    13861 tcatgaccgt tctgttcaac agaggatgtt caatattctt ggattttcga aagaagaggc
    13921 gcaaaggcaa tttggttttt tgttagaagc ttttacctac ggtgctccgc cccatggagg
    13981 tatcgcattc ggatttgaca gactatgtgc attgtttgga ggtacagaaa gcatcaggga
    14041 ttatattgct tttccaaaga acaacgcggg tagagatgta atgattgaca caccctcaca
    14101 aatccataaa agtcaattgg atgagcttgg aattacagtt ctgagcaaga aaaagaaaga
    14161 ataaagacta ttgttacacg gttaccagtt tttgggaaat ccataccttt cattttaata
    14221 agtatttaaa ccccctgtat ttttccagtg gttctgaaaa tagaaatgca ttagctgtat
    14281 atggtattta aaccccctgt agtccccctt aaaacaaggg ggacaattct ggtttaattt
    14341 cccctcctta ttttaaggag gggtttgggg tggttaataa ttttccagtg gtggttctga
    14401 aaatagaaat gcattagctg tatatggtat ttaaaccccc tgtagtcccc cttaaaacaa
    14461 gggggacaat tttccagtgg ttctgaaaat agaatgcagt tgttacgttg ttataaccct
    14521 actactccga taatacttcc actgcaggaa ttacgcgaag ccccggtaac actagccaaa
    14581 acattatcct ctcctcttaa ccgcagcgct cccatcagcg caaagaccaa agcttcttta
    14641 aattctatga tcttcctgtc aggaatacat acttcaatgc cgccaagctt ctttttcaat
    14701 aattcaataa aatataaatt gaagacaccg ccaccggtaa ccaggatttt ttttattctt
    14761 tcaactctaa atatagagtc tgccagaata tctgaaaaat gttcagcaaa cgttctcaga
    14821 caatcatttg cgctaacttc aaatgctttc actggaatca aaatatcttt ttcaatgtct
    14881 tcaaagccca gggacttagg agcatttaat tgatagtagt cgaggtttgt caaaaaattt
    14941 gacaactcag gatttacaga accttcgcga gccatttgcc cgctctcatc taattcaaaa
    15001 cctaatattc tcgaataata attaaaaata atattgaggg cgcaaacgtc ataggcgatt
    15061 ctctgtccat tgaaatcata agaaatgtta gaaatacccc caaggtttag gcacgcatca
    15121 tagctgctga acaaaagcac atctcccact ggaaccagtg gcgctccttg ccctccaagc
    15181 tgtacatcaa gtgaccggaa atcataaatt acaggaactc cggtaagggc atgaacgcga
    15241 ttggctgatc cgatttgtgt ggttagcctt tttttaggtt ggtgaaaaac tgtatgacca
    15301 tgactgctga taaaatcagg tttaaaatta tttttagtga tgaacctatt aacggcatta
    15361 ccaaaaaaat cacctagcgc taaatccatt tctgccaaat caatggccga agattgagtg
    15421 gctttagcca gtgtattttt tatagtctgt gaataagcaa tcgattcaca gcagagaatt
    15481 tcaaatgacc aattattgtt gtgatcagtg agcataaccc cggctatgtc cagaccatca
    15541 agggaagttc ctgacatcag cccgataacg ttgtaaacac tattcattga attgcactat
    15601 tttggtaata tagtaacaaa tttcaccaaa attaataaag tgaacgcggt catcgacatt
    15661 ggtaattcaa gaatcaaatt gggcctgttt caaaacggga aaatgattga ctgtaaactg
    15721 gccaataatg aagatgaagc ttctctttta ttaggaaaga ttgaaatcaa gaaaatgatt
    15781 gtaagttccg ttaggaagga aaatgattgg attaacaatt taaaggtggg agaaaaaaaa
    15841 gtatttcttg gtcccgaaac acccctgcca ataaatatag tatacgatac acctgacaca
    15901 ttagggacag atcgaatcgc ggctgcaacg ggggcatttt tacgatatcc aaaaaagaat
    15961 tgccttgtaa ttgatttagg aacctgtatc acctacgatt ttcttgacga aaagggaaat
    16021 tttaatggcg gagcaattag cccggggata agaatgcgat tgaatgctct gcatcaacaa
    16081 acatcaaatc tgcccttaat tgaagcatgg gatgctaaaa ttcccttaac tggaaaatct
    16141 accaaagaat ccattttgtc aggtgtcata ataggcgtta aacaagaaat tgagggattt
    16201 attcgttcat atcatgacaa agtcgcaaat ttgcaaatta ttttcaccgg tggggacttc
    16261 aagatggtag aaaatttgtt ggagagtagg ccaatctggg caaaccatct cgttttagaa
    16321 ggattacatg gaatactgga acacaacaat tagaaagcga agcttcaaaa tactattcgt
    16381 gggattcctg ctcacgcatt cgctgatatc cgcgcaaaca atttctacat attcaggatt
    16441 gggattggga gatataatca ggccggcttt ttcaaatcat attggaaacg gattgggatt
    16501 aagcaaagga gaatattggt ttgtgaacca gatgaacccg gccctgcttc cttacaatca
    16561 ggtcacaacc tttcaggcag gtttgggttt tgaaaccaag acagtcagtc aaaatgatct
    16621 tcaggatact tacagctctt tcaatttgct ttatctgggg acggcgattc cggcaaaggc
    16681 agggaaatgg acatttgctt ttggcgttca accgtacagc agagtattat acaattttgc
    16741 aaccagcata gatatacccg gcagcggagt aaccgcggaa ataactaatc agggctctgg
    16801 cggatttaat caggcatatt tctcaaatgg attcaagatt tggaaaaatc tttccttagg
    16861 aatcaaagta tcatatttat tcagttcttt aatcaaggaa agcacaacca ttattggtga
    16921 cacactggat ccatcccgat cagcacgttc agttttgcag agaactaatg caaaggacgt
    16981 gattttttct accggaattt catacaaaca caacatcaac aaagacatct ctgcaaacta
    17041 tggaatcact ttcgatccgg aaaccaaaat ttctgcggaa agatttgaac ggattgatat
    17101 acgaactcct gcaggtactg tattagatat tgatacggtc gctaataacc tgtccggcag
    17161 tttcagattg ccgcaaaagt ggggtgccgg aatcagtttc aatgaagggt ttaaatggtc
    17221 attcggtgcg gaggttgaat accaacagtg gagcaggttt aaagattttt ccggcaacaa
    17281 cgattacttc caggatggat tttcaatcag ggtaggtgga gatattgtgc caaatccaat
    17341 agccattgaa aattatctca accggattac atatagactc ggcctttctt atgataaact
    17401 tccattcaca atttccggaa aggatattgc cgagtatggt gttaattttg ggtggtcaat
    17461 gcctgtgagc agaatttcgt atattgactt cggaatgcga tttgcagtaa ggggctccac
    17521 caggaacaat ttgatcaggg agaattattt tcagctgcat ttgggcttca cttttaatga
    17581 tcgttggttc cttagaaccg cttatgatta attaaaaatg aaaaaattat tactttgtgt
    17641 tttgctggcc gccgaaacaa ccgtatcctt tggccaaaat tgcaataatt ggaattaccc
    17701 tgaggataaa aaaacagttg aggagaaatt agttctttat tccgataaca aaagatttgg
    17761 taattttaaa gaatcagcca aacatctcaa ttggttgctt gtaaatgcac ccggtctcca
    17821 cttatcaatt taccagaatg gctctcagat ttatgaagag ttggcaaacg cggaaaccga
    17881 ccccctccgg aaaagaatct acaaggattc gtcacttttg atttatgatc ttagaataca
    17941 attttgtgga gatgagaaaa atgttttaaa cagaaaatct ctggcagctt accatttttt
    18001 taaagatgac aaacaacgcc tgggagaatt atatgaatta tttaaaaaga cttttgacct
    18061 caacaaaaat agtgtctggg aaaatatcct tgttgcctac ctggatgttg tgaggagata
    18121 caggtccgcc ggtggaaata tttctgatga aatggtactt gaggtctatg atgaaatttc
    18181 aggtatcatt gagaataata ttaacgaagg cggaagtttt gaaaagtatg aaaaatatca
    18241 ggaaatgtta ttccagattc tgttacttat tattcctctc gactgcgatt ttattgattc
    18301 aaaccttgtg cccagattaa aagccaaccc tgacgatttt gacctttcaa aaaagatagt
    18361 tcagctttct gttattgcaa aatgtaccga aacagacttt ttcattgatg cagcaaagaa
    18421 agttcaggcc agggaaccaa cttatggatt agcctgggcc atcggaaatc gatgtttggg
    18481 taacgagaat tttacttgtg ccgtccaatt tttcgaacag gcaattactc ttaccgatga
    18541 aaaggagaaa atagcggatt tgtactacaa gctggctaac gtcgccttca ggcaggatga
    18601 ttatccgaag gcaagagaat tggctttcaa aactatagag acagaccatt caagaagaga
    18661 cgcttactcc ttcatcggaa aactttatgc tgacagctat gaaagatgca aagaggaagt
    18721 tgaccgtgtg aaagaccggc tcgtattcat tgccgcctat cagatgtttc aaaaagcagg
    18781 ggacatggaa gctatgaaat ttgcaaaaga acaatttccg tcaagagaag aattatttga
    18841 acgtaactac aatgccggac agtccatgaa gctaacttgt tggattgatg aaacagttat
    18901 acttcaaaca agggattaat attttaaagg tgaaatcttt aacttttatt ttttggagta
    18961 caagcttctt cctgctatgt tcaggatgtg aggaaaaaat agatcccgga aaatttgttg
    19021 aatacgaagg gccggttaca acgctggacg gcgctgaaat tatgcacagt gattcagcaa
    19081 taattacagc aaaaataatt actgacaaat tacttcaatt tacaaatgga gatcaggaaa
    19141 tgccatcggg attccatata actttttttg atagtgaagg aaaaccaaac gcaacattgc
    19201 gtgcagacta cgcctataat gataaacaga agaacttatg gaaagcaacc ggagctgttg
    19261 aattgaacaa tataaaaaca ggagaaaagc tgaatacgga agagcttttt tgggatccac
    19321 cgggagaaag aatttttaca gagaaatttg ttagaattga atcggaggat caaattctgt
    19381 tgggcgaggg tttaacggca tcccaggact tcacaactta caaaattcac aaacttacag
    19441 gtgagatcgt atttaatctc gacggagagc catccgcaaa tcccagaaaa tgaaaatcag
    19501 aaacacactc ctgtactcag catcaatcgg catctttgct attggtattc atcaggggct
    19561 aactgtgggt tgggcttatt cctattggat ttttatgatc tccgtttctt tcctgctaat
    19621 tttgaaagtc ctaacaaata aagaaaagga gagataattt attggatccg cttcccatta
    19681 taattgtttc attaattttt tcggcacttt tctccgggct cgaaattgct tatgtaacaa
    19741 gtaataaatt ggctcttgaa ttactgggga aacaaaatac gttaattggc agggtaatat
    19801 ctgtatttca aaataaccca tcccaattca taggcacaac ccttgtcggg aataccctgg
    19861 cgctggtggt ctatggaatt tttatggcca aaatattaga gccggtattt gcagccaatt
    19921 ttcccgccgc actccagaac caggcttcga ttctcgttgt tcaaacaata gtcagcacta
    19981 ttattgttct tgtaaccgcc gaattcactc ctaaaagtat tttcctgatc aaccccaacc
    20041 ggatgctttc tttacttgca ttcccaatgg tgatcattta ctatttaatg tatcctgtag
    20101 tatttgcaat tgtgaagcta tccaaactca taatcacaaa agttttccgc agagagtatt
    20161 atgaagacag accagtattt ggattaactg atctaaatca attcataaag caattgaaag
    20221 agagcggtct cgatgaaggg aaagtaggct tggatacaaa aatcttccat aatgctctgg
    20281 agtttaaaac cgtaagggtg cgcgaatgta tgataccacg cacagaaatt acagccgtgg
    20341 aagtttacga tgccatggaa actgtaaaaa aagcttttgt tgattcaggt cattccaaag
    20401 ttatagttta cagagactca atagatgaag tgctgggata tattcattca tctgaacttt
    20461 tcaaaaagcc aaaaagtctg gaagatatca ttactcctat tatcatcgta cctgaaacaa
    20521 tgttggccaa tgaattgatg atacggttta ttaaagaaca taaaagcctt tcgttggttg
    20581 tggatgagtt tggtggaacc tccggtttgg ttagcataga ggatattatt gaagaaattt
    20641 ttggagaaat tcaagacgaa cacgacgatg aagatttgat agaacagcaa ttggatgcta
    20701 acacctggtt gttgagtgca aggcatgaga ttgattacct gaatgataaa tatggttggg
    20761 aaataccaac aggtgattat gaaactctgg gtggattaat tttgtcagcc aaagaggact
    20821 taccgaagaa agatgagcag gttgtaattg ccccttttcg tttcactata attacggttc
    20881 aggatatccg gattgacaca gtaaagcttc atattgacca aaaattgcgc ggcttaaaac
    20941 aggggtctta accagtagtt ggaattttga ttttcccacg atttagaagt aattttgcgg
    21001 tctttatatg taaataacat ggctttaatc aacaagttga gggagaaaat gggtagagtg
    21061 gtggcatttg ccatcggttt tgcaattctc tcatttatac tggcagattt aatgggaccc
    21121 agctcattta ttatgggtgg aaataacaga aacattgggg aaatagcagg agaaacgatt
    21181 acgctggatg aatatcaagc cgaaatagag gctttggttg ctaattatac tatgaatttg
    21241 ggaagaaatc cgtctgagca ggaaatggcc acgctccggg atcaagcctg ggagttgctc
    21301 atcgttaagc atgctttcca gaaacaatat gaaaaaatcg gacttgatgt gcatgaagat
    21361 gaagttgtcg acatggtgca gggaaagaat gttagccctg caatccagca ggcgtttgtg
    21421 aacccggaaa ccggagattt tgataaacaa agactgctgg catatcttca gcagattaaa
    21481 ggtgctccac aacagcagca atatgcatgg tatctctttg aaaaagattt aaaaccaagc
    21541 cgtttgagat tgaagtacga gaacttatta atgaaatcaa cctacattac aaccgaagaa
    21601 gccaaaaaga attacgaaaa ccaaacagct gtggcggaaa taaaatatct ctacgttccg
    21661 tactactctg taaatgattc actggtttct gtaacagata aggaattaga aacttatctt
    21721 ggtgctcatt cagcacagta tcaggctcag gaaagccgct catttcaata cgtaacaata
    21781 cccattgaac cttcttcaga tgacaccact tattttagag aagagatcga agaattaatt
    21841 ggtcaaatca agaatattga agacgattca gtttttgcca ttattaattc tgatgggttg
    21901 aatccattca gagagtatag aattaatgaa ttacccggga atgtagcctc caggctcgat
    21961 gaactggagg aaagaatggt gatcggacct atattcgaag gtacggcatt taaattgttt
    22021 aagattacat ccataagtga agatacaatt tttaaagcac gggtaagtca tatcttgttt
    22081 aaatgggagg aggaaagtcc ggaagaaaaa gctaaagcaa gaaaagaagc tcaggatgtt
    22141 cttaatcaac ttcaaaatgg agagaacttt gaaatcctgg ccaggcttca tagtaaggac
    22201 aattccgctt cgcgtggcgg tgatctgggt tggggaacca aaggacaaac ctgggttgct
    22261 gcctttgagg ccccggtttt caatcagaaa gagggaggtt taatcaatag gatcgtggaa
    22321 tcggaatttg gtttccatat cataaaggta accgaaccgg ccaataacca gattttcaaa
    22381 attgcaacca tctcaaagga agttatcgca agtgatgaaa ctattgagaa ggcatttaga
    22441 agagcaggtc tattccaatc aagcataact gattatgatg aattcataag taaggccaag
    22501 gcagattcca ttactttgca aacggctacc ggagtaactc ctaaccaacg taacattccg
    22561 gggctcggtc aggcaagggt aattgttcaa tggctataca ataccgcaaa agttggaagt
    22621 atttctgatg ttttcgaact tgaaggtact tatgtcgtag ctgtaatgac ttcacgaaag
    22681 gtggagggta cttctacact caatgatgta agaacgcagg tgtccctgaa agtaaaaaat
    22741 aagaagaaag ctgacattat aatgtctaaa ttatcggaag cgtcagggac attggacgaa
    22801 atggcaacag cgtacggttc tgatgcggtc gttcattcct ccagcgacct taaattaaat
    22861 actaattttc ttccatctgt cggtttagcc ccggaagcaa ttggcaaaac ttttactctt
    22921 caggggggag aacgaacaat tcctattcca accgaaaatg gcgtggtgat agttgagatg
    22981 ataaatttta ccctcgccgg cgaaatagca gattatacca catacaaaac acaaatgaag
    23041 caaggcctca ccagcgggat ttcctatggt ctttctgaaa caataaaaaa acagtcagat
    23101 attaaagatg agcggtataa attttactaa tctcaaccaa aaaggaagcc tttagagtaa
    23161 tgagtgaagc atttgatgac ctaagaagaa ctttggatga gtcttatacc tggccggtac
    23221 tttatatgtt taaattcatt gctccaaaag ataagataga taaattaaaa actctttttc
    23281 ctaaaaatga gtttattgag cgggagtcaa aagaagggaa ttatcttagc ttaacttgta
    23341 aaatattagt aaactcgagt gaagaagtaa ttcgtgttta tagacaggca agcaaaatag
    23401 aaggagtaat ttccttataa gaaaatttga cttcagtgaa aaataaattg gccgcgattc
    23461 taatctccaa cctcctcttt ttttcatgcg attcagcaag taacaatcga tcggaagctg
    23521 atgttacgaa caattcgaat gcaagaacag aaaaatcaat cgatagctct gcatttgaat
    23581 tactggtaaa taaaattgaa gaccccgaaa gaaagaattg gcagaatcct gatctggtta
    23641 ttcaggaact gggaaatctg gaacagaaaa ccgttgttga tatcggcgca ggcaccggct
    23701 acttcacatt cagaattgct cccttagcag caaaagtaat cgcagtagat atcgataaaa
    23761 gatttttgga tgtaattgac gatagaaagc cggactttga tcaggagtgg gttgaaagaa
    23821 ttgaaacgcg gctcaccctt gaagatgatc cagggataac tgaaaatgaa gcagatattg
    23881 ttatcattgt gaatacatat cattttctga aaaacagggt aaactatttg caaaaagttc
    23941 gtaatggcat gaaggagggt gggactatcc ttatcattga tttcaaacag gcggatatgg
    24001 aagtggcacc cccgggagat ctaatcgtac catctgatac tgcaattgca gaattaaaag
    24061 ccagtggctt caaaaatatt actgtggatc aaatgagtct gaaatatcaa tatataatta
    24121 aagcaacctc ttgatatgtg gaaagaagaa aacaataaac taaccagaac atttactttc
    24181 aaaaacttca ccgaggcctt cggatttatg actaaggtgg cgatagaagc cgagaaaatg
    24241 aatcaccatc cgaactggtc aaatgtttac aatcgggtga ccattgaatt atcaactcac
    24301 gatgcaggaa atgtggtcac agagaaagat cgaaaattag ctgagataat aaataaatta
    24361 gttactgaag gttgacaggt gcaaaacttt tcatagttcc tactcccatt gggaaccttg
    24421 gagatataac ctacagggcc atagatgttt taagtaaagt agacctgatc cttgcggaag
    24481 atacccgaaa gacaggcgtt ctcctcaagc attatcaaat cagcaagccg gtaaaaagtt
    24541 atcacaattt caacgagcac aggcaactgg ctgctattat tgaacaattg aaatcaggca
    24601 ggagtatagc cctggtttcg gatgccggaa ctccaggcat ttcagacccg ggataccttt
    24661 tgattcggga atgtctgaat gaaaaaattg aaatagaagc attgccgggg cccactgctt
    24721 ttatacctgc attattgaaa tctggttttc cctgcaatag atttattttt gaaggatttc
    24781 ttcctcacaa aaaaggacgg atttcaagaa ttgaagcttt gaaatcagaa cacagaacaa
    24841 ttattttcta tgagtcgcca cacaggctga ttaagacgct tgagcaattt tcaaaaatat
    24901 ttggaatcga cagagcaatt tccatttccc gggaaatatc caaaatgtat gaagaaacat
    24961 ttaccggaac cgtttcagag gctcttgatt attttagatc ccaaacaatc aaaggggagt
    25021 ttgtaattgt agttagtgga aatcaaaaaa agtctgatgg aaatgctcct gaataatgat
    25081 tcttatcatt ggacaaaatt gaataccgtt ctacattaag ggtcagaata ataaaatata
    25141 acgccaatga aaaccttaca gaaaaacagg gaaaatttac ctgtaatgcg ctcatcccta
    25201 tgggatatgt ttgattttga cagacttttt gacagagacc tgatgaacta ccagtggttc
    25261 aacaaggtac cggcggccaa cattgttgac aaaaaagaca gttttatgat caatctggct
    25321 gtaccgggca tggagaaaaa agacttccat gttaagatag acaccggtct attaactatt
    25381 agcgcggaaa aagaagagaa gtttgaagac aaggacgaag aattcaccag gaaggagtat
    25441 aactacaact cctttacgcg ttcctttgtt ttacccgaat ccgtaaagac cgatggggta
    25501 caagcaaagt atgaaaatgg agttctcaac ttaaccctgc caaaaaagga agaagtgaag
    25561 aagtcatcta agactgaaat caaaatcaat tgaaaatcat ttcatgaggc gggcgattca
    25621 ataaagcccg ccttaatttt ttaaaaaaca tacattttat gcaaaatctt ggtgttaatt
    25681 atagctatat tcttgaaacg agcctgattg accttcgaca aaaaagcaaa gtctggcttt
    25741 ctgaagttga actctggcta attgagctcg cattttttcg caaaatgcta agattaaaca
    25801 aagagagatt aattgaagga aatacttcaa ttgagaatga gttagatcac tatcgggaac
    25861 tcatagatta ttacgaggtt agattgctta gggatctgaa aaaaaggttg aaaaaacatg
    25921 aaggaaaatt ggttaaatta atggacggac acccggtaca ggatgaagaa tccttcaggg
    25981 agaatcataa aaatatcggt caggtagttc atgcatttgg gaatgagttt aggaatttaa
    26041 agaaggaatt cttaactttt attgagcccc aaaagaataa ccgctatgtc tcgcatatta
    26101 ataccgattg acttttcaga atgcagtttg aatgctcttg aatacggcct taaatatgcg
    26161 gagaaaacgg atcccgaacc gcgaataatc atagctcacg gttacaatat tccaattccc
    26221 gccgcggaat tttcaatgag cctcaacgcg actgttttgg atgaattcaa gaaagatgcc
    26281 gaagcaaaat tcgaaaagct caaagaacga tttccggcat taaaaaagaa ttctgtaaaa
    26341 tttgacgtta aaatggcttt tgttcttgat gcaattttga aatgtgttca tgaagaaaat
    26401 ccacatttga taataatggg aactaaagga tcgagcggca tcaatgaagt attattcgga
    26461 agtaacacct caggaataat aaagtcttct aaaatacctg taattgctgt acccgagaat
    26521 ttcaaagcgg gagctatatc tagaataaca ttggcatttg acaataaaga gcttgaagac
    26581 tcttcaattc tggaaattgt gaggaggtta tcctcaattt atcatgcgcc ggtagacatt
    26641 cttcatattc aaaataaaag tgaagaaaat gatgatggcc ctgccatgtt agcaaatttt
    26701 tttgaaggag tacagcatga gtttcacttg attgagggaa atgatatcgc cgaagagatt
    26761 gaaaaattcc ttgataaaag caactccgac cttttggcca tgattcccaa aagacacact
    26821 atcatagaac gattattcac tcgaagtata acttcaaaaa tagctcaaca tacaagaatt
    26881 ccactcctgg caattccgat caaatagcag ttgcgtgaag acttatagct tcgtcaagta
    26941 ggctttttaa agatttatgt cgcaaaatat tttttacttc gttctggcgg gtaataatat
    27001 ctccacaaaa aataatacca ttttcaatta gcgatgtctt ttcttttctg gtcaatttgg
    27061 taaatactgt aactggatat aacccggagg aaccgatttt cactttcaaa ttattttttt
    27121 ccggatagtc ccatcccatt aacgaaaggc cggcacacgc agaatacctc acggcatcat
    27181 ctgtaaaacg agtattggtt actatccaac ccctgaagtt aaatttagat ctgttgttgt
    27241 ttttgaaatt tattatatcc tgataccttg aattaatata taacggaact ttgacatcac
    27301 ttttaaggct actgcgattg tgaaatttac attcgatgat ttcaatcatg ttctcctttt
    27361 tagccaaaac gtcaacctca tgttgcacac aggcaccttc taatatcgcc ccaatttcaa
    27421 tttgataacc cattctttca aatagctttc ctacaagtac ctcaaacgga tagccggaag
    27481 gacccagttc ggtgatagcc tctttaagct tatattggga agcgactaaa atacttttct
    27541 tccttaattg gttgaaggca acgcgataaa ttttgcgggt agggattccc gggtataata
    27601 acttacttat ttccttgtta atctctctga taatttcatc tgacgccccg gaacgcttca
    27661 gggaaacaat cagcttttcc ttgttgaaag gttctaattc acccgtgttc ttgacaactg
    27721 aaatttcctg tttcaaaacc tttaaaaata acccgatttc ctggtattca tgcacatttg
    27781 gttaataata aacaaaatta tatatttgct tgtgcataca aatataattt gaatattaaa
    27841 gactctccat attgcaattg cctgtatttc agtcttggtg ccatgtcaag acaaatccaa
    27901 aaaatagccg aagaagagtt cagggaaaca ggcttgtcgc cttctcatgc cttcgtgttg
    27961 atgagtgtga atgcgcgtca ggaaattaat cctggtgaaa ttgccaaaga attattgctt
    28021 gccccttcaa cagttacccg gctaatcgaa aaactggaag ccttgcagtt ggttcaaaga
    28081 gagaatgaag ggaagtatat gaaagtaata acaacctcca aagggctgga gaaagacatc
    28141 aggatcagaa aggcttggga gaatttattt aagagatata cccataccct tggtcagtca
    28201 ttcagtacta aactaactga gaatcttcag gaagcatttt caaaatcaaa ccgaaataag
    28261 tcatgaaaac agtaggaatt ttaggttcag gtccggtggg caaagcattg gcaaaaggtt
    28321 ttagcaaata tggtcaccag gttatgattg ggaccagaga taaatcccgg ttagacgagt
    28381 ggatcgttga gaatccttac gccaaaagtg gaacttttga tgaagtagca aagcatggag
    28441 agatattagt gctagcagtc gcggggagag tggccaaaga agttctcaat tcatcgggaa
    28501 ggagtaacct ttcaggaaag gtgatcattg atgcaaccaa tccgatctca aatgatcctc
    28561 ctcaaaatgg ggtccttcaa tttttcacgg acctaaacca ctccttaatg gaggatttgc
    28621 aaaccgaata tcccgatgca atgtttgtta aagcctttaa cagcgttggg aatccattta
    28681 tggttgatcc tgattttgaa gaaggcaagc ccaccatgtt catttgcgga aatgatctga
    28741 aggctaaaag tgaagtggaa accattctcg aaaaatttgg atgggaagtt gaagacatgg
    28801 gaggagcaga agccgcacgc gcaattgaac cactctgcat actttggtgt ttgcctgggt
    28861 ttttgcggaa ccagtggaat catgcattta agcttctcaa aaaataggtc cctgggaagt
    28921 tttctaagaa ttaattaaga ctacatttgc aagattattg acttgagcaa ggtcaggaat
    28981 agttcggtac ttaaatttgc agacattttg acattatgat gatatcattt cttatctacg
    29041 tatttttact ttttcctcaa acccttgacg ctacagatat tattcgccgc gcggaccaaa
    29101 aaatgcaggg gagcagcaat atggctgtaa tgactatggt aattcaacgg ccggaatgga
    29161 gcagggaaat cgaaatgaag agttggtctc tgggtatcaa gaaatcttta atcctgatta
    29221 cggcgccacc tcgggatgca ggaaccggtt ttctaaaaag agataaagaa atctggaact
    29281 ggcagccacg cattgatcgg gtgattaaac ttcctccctc tatgatgatg caatcctgga
    29341 tgggttctga ttttactaat gatgacctgg ttaaagaatc ttcagtggta aatgattata
    29401 atcatactta caaaaaagac accgtaattc gaaattacaa ttgttacaaa attgaaatga
    29461 ttcctaaacc tgaaactgcc gtggtatggg ggaaagtaga tgtttatatt gaaaaagatg
    29521 aattactcca acttttgatt aagtattatg atgaggaagg ctacctggtg aataccatga
    29581 ttctttcaga aataaaaaat ctgggtggaa gatcaattcc aactttaatg gaaatgattc
    29641 cggcagatga gccgaaccat aaaaccataa tacgatataa agaaatgagt tttgatattg
    29701 acattgacga ttcattcttc agtcttcaga atatgaaacg ggttaaataa taataacgtg
    29761 gtgttaaagc tggcctggcg caacatctgg agaaacaagc gaagaacgat aattaccctc
    29821 tcttccattg cttttgcggt atttttctcg gtaataatgc aatccatgca attagggtct
    29881 tatgagagaa tgattgaaaa tgttgtcaaa ttctacaccg gatatgttca gattcacagc
    29941 gaaggctact gggaagaaaa aatccttgat aatagttttg agcccaactc atcattgata
    30001 tctgagatcg caagattgga aggagttgag caggttgttc ccaggttgga gtcatttgca
    30061 ttatcttctt accagtcgca aaccagggga gccctgataa tgggattcga tccggtcgcg
    30121 gaaaaaaaat taaccgcact tcatgcaaag atcattgaag gcgaatatct gacagaaaat
    30181 gacaatagtg tcttgttgtc acaggggctg gctgaatatt tgaaaataaa ggtttctgac
    30241 acagtagttc taatcggtca gggttatcat gcaatcaatg cggtgggaaa ataccacgta
    30301 aaaggaatta ttaaatttcc tgcaccggca atgaacaatc agcttgttgc aatgccaata
    30361 cttctggcac aatggttcta tggtgcggaa aacaaataca ctgctttggc actcgttctg
    30421 gatagccctg ataatgtgca aaaagtaagc aaagagattg tttcaatact tggaagggaa
    30481 aatttagagg ttatgacctg gcaagaaatg atgccagaaa tagtacagca gattgaactg
    30541 gattacgtca gtggccaggt aatgctttgg attttatacg ctgtggttgc ttttggaatt
    30601 tttggaactt tcctgatgat gaccaatgaa agacggtatg aaatgggaat aatgatcgcg
    30661 gttggactga gaagaatcag aatgcagatg attatgggaa cggaattatt gttaatgggt
    30721 tttagcggta ctctggcagg tattattatg gctatgcctc ttttgatata tttttatttt
    30781 aatccaatcc gatttacagg agaatatcaa aaactatccg aaagttttgg atatgaagcc
    30841 atactaccat tctctatgga cgccggcatt tttctatacc aggcaatgat ggtttttctt
    30901 atcacaatca ttttgggtat ttatccgcta aataaaatac acagattaag aattgccgat
    30961 gcaatgcgaa attagaaaat tatgattagt aaaatagcct ggaggaatgt ttggagaagc
    31021 ccggcaagaa gccttgttgt aattggagcc attgtactgg gagtatgggc gctggttttt
    31081 accattggat ttgttaattc ctgggtaatc aaatttgtag agaacagcgt ttctcaggaa
    31141 tactcaaatt ttcaaattca cgaagtggaa ttcaagaaag attcggaaat gaaattcttt
    31201 attccggagg gagataaact actcgtatca ttgcaagagc atcagggtgt tatcgcggta
    31261 accggaagaa tgaaatctaa cgcgatgata tcctctccta agaccgccac cgggggaatc
    31321 atttatggaa ttgaaccgtc agatgaagcg gaagtaaccc ggctggattc ggcaattgtt
    31381 gatggctatt atttcgatgg aatcagaaga aaccccatcc ttatcggttc aaaacttgct
    31441 aagaaactaa aggtaaaagt acgctcaaaa gtggttgtta cccttcagga catcaatgga
    31501 gatattacag ctgcttcatt tcaggtggct gggatatttg aagtgatgaa tcccatttct
    31561 aatgaaagta aaatatatgt tcgaagatcg gacctttcaa gaattgtaaa cctgagtgaa
    31621 atccatgaaa ttgccggcct tacaaatcct tccggagatg atgaaattat tattcccgaa
    31681 ttgaaagaaa actattcggg cctgcaagtt gaaggatgga aggatttggc tccggaactg
    31741 gacctgatgc tgggccaggt aaaaatccag gtaggtatta tcctcggaat tattatggtg
    31801 gctctggggt ttggaatagt gaactcgatg ctgatggcgg tgctggaaag atttaaagaa
    31861 ataggaatgt tgaaagcagt agggatgaag gggagaaggg tcttttatat gattgtgatg
    31921 gagacaattt ttatggccct tgtaggcgga ccaattggat gcttgcttgg atacatcaca
    31981 atagaaatac ttgggaagta tggattggat ttatcgaggt attccaaagg gttagctgag
    32041 tttggaattg atgttgtact ttatcccgca gtaactcctg aaacttatcc attattgaca
    32101 ataggggtgg taatcaccgc attagtagcg acgctttatc ctgcaagaaa agcggtcaaa
    32161 cttaaaacta tagacgcatt acataagatt tgatatgacc atcataaaag gggaaaatat
    32221 aaataaaatc tacctggaaa ccgtggtgcc tgtaagagca ttaaatgggg tagatctatc
    32281 cattgaagag ggcgagttta ctgcaattgt gggtccatcc ggatcaggta aatccacact
    32341 gttaaacatc attggagggc ttgactcacc tactgaaggt aaaattacgg tggctggcat
    32401 cgatatatca aaacttaagg aaagtaaact catagatttc agactgcata acattggttt
    32461 tgtgtttcaa tcctataatc tcattccggt gcttaccgct aaagagaatg ttgaattcat
    32521 aatgctgctg caaaaatggc cgaaacaaaa aatggaagaa cgcgcaatcc aacttcttga
    32581 tgcaatgggc ctgtcggata aatatcataa caagcctatc gaactttcag gtggtcaaca
    32641 acaacgggtg gcagtagcaa gggcattggc gtcacgtcca aaatttattc ttgctgatga
    32701 gccaacggcc aacctggatt caaaatcaac agagaacctt ctggggttga tggaaaaaat
    32761 gaatcatgaa gaaaatgcta cgttcatttt ttcaacccat gatcaaaggg tgattgaaaa
    32821 ggcacaccgt ataattactt tggttgatgg aagaatagca tcggatgaat tacacaccgc
    32881 cggaaatcat gtcaggtaga ataatccttt tatccggtct tattttttct ggcataaata
    32941 cttacggcca ggaagaggaa acaggaattg agcacaactg gctttacaat ggttatataa
    33001 aagatttact gactgtaaac gttcttgatg acagtactct ggttgataat ctaatacata
    33061 accggcttaa ctttcaatgg tggccgaaag aatatctaaa tatttatgtt gaagtaagga
    33121 acagaatttt ttttggagat ttagtaagcg cttaccccaa ttacagcgaa ttgattgaca
    33181 ctaatgatga tttctttgat ctatcatgga ccatcgtaga ttcaaaggat ttagttatcc
    33241 actccatgat tgatcgtttg tatgttgaat atcacaaaaa agattgggag atccggttgg
    33301 gcaggcaaag aatcaactgg ggagttaacc ttgtttggaa tccgaatgat ctttttaatg
    33361 ctttctctta ttttgacttt gattatgaag aacgcccggg cagtgatgcc attcgaatta
    33421 aacgatacac cggatttgct tcaagtattg aagtggcaag taattttagt aactcttttg
    33481 atgaattggt tagtgccgcg atgtggaaga ttaataaagg cggctacgat tttcagcttc
    33541 ttgcgggcaa atcatttcag gatattgcca ttggtttagg gtgggcaggt aacataaaag
    33601 gagcggggtt taaaggagaa gcaaccctgt ttgaacctta tgagaacact gtttattcaa
    33661 cagtaatact aacaaccatt agccttgatt actcctttga aaattcactt tacttgcatg
    33721 catcgggcat gtacaattca caagggctaa cagagccggg aataggagcc ctggaaccta
    33781 ttctttcgcg aaaccggctg acggtaagga atctttcacc atacaaatgg tcgacatttc
    33841 ttcagcttag ttatcctgtt catcctcttg taacaaccgg agttgcagca attttttatc
    33901 cgggggataa tgctttattt ctgaatccaa gtataggaat ttctctgcct aaaaatttcg
    33961 atcttgatgt attggcccag gtattcttca atgatactct tttcagtgat tacaaagctc
    34021 tggcaaaatt attctttgtc agactgaagt ggagtttcta attcttgctc ttctcaattt
    34081 ttaattccgc cctgcggttc ttcgaatgtt cttcttcctg acattgaact ccgtcgctgc
    34141 aacggttaat cagcaactgt tcaccaaagc ctctccaggt tattctgtct tccctgattc
    34201 ccttttgttt cagatacgca actacagcca aagctcttcg gttggagagc gtaagattat
    34261 actcgaacgt tccctgggca tcagcgtggg cacttacaac gatgtaggta tctttatgac
    34321 gcttcaggtt cgtaatcatt tgattcaatg ccggctgggc gtcgggtttt ataattgcct
    34381 tattgaaatc aaagaagatt acgtctttag taacatagac ttcctctaaa acaaaatcat
    34441 ttagcaatgt atcggcaatc atgccttcag tattaagttt gaagttgctg gtaacatgat
    34501 caatcttttc agcctggata atgtaaagtt ggtctggttt taagacaaag acatatttgc
    34561 cgtcctcttt agtttccaga attgtgaagg tgttctgagt aagattttca agagaaacgg
    34621 tgacatcagg caagagctcc tgggtttcat tactaaatat tcttccttgc gcgaaaagtt
    34681 catgccgcca catgaaaaca tctgcagtat cagtttgaaa tgaatgtacg accgtagaaa
    34741 tacgggttgg aacttccagg ccgaaattgt cctttttggc ttgtaaatca aaataggtat
    34801 caagtggcag gtctacctgg aagtacccgc tttcatctgc tttcaaaatc gtataagttt
    34861 ggcgggcaga atcattaatc ataatttccg cattgccaac aaattctttt gttcttgatt
    34921 ccagcaccct gccgacaata cttttatagc gaacttcaaa ttgataaata tcatcactgc
    34981 ctttccctcc tggccggtta gaagaaatga acccttttct gccatcctta ccgatagaaa
    35041 atgagaaatc gtcaccggag gtattcatgg gaaatccaag gttttctata ttaccaaatt
    35101 ggccatcaaa cctggtagag cgataaatat ccagtcctcc aaaacctcca tgtccgttgg
    35161 atgaaaaata caggtggccg tcttccgaaa taaagggaaa gaattcatcg ccggcggtgt
    35221 tcacttgtcc gccagcgttt acaggctcag accatgaatt atcaatcctc tgacaaaaat
    35281 aaatatcatt gccaccaaaa cctccgggcc tgtctgatgt aaagtaaagc gttaggttgt
    35341 cagcacttag tgaaggatga gctgtagaat actcattatc gttatagaca aatggcttta
    35401 catttgacca ttcgccgccg ggattaaggc tggcggaata taactttaat ttgattttcc
    35461 cgtcttcact ttctcctttg cgatatgaat aataattact ccgtgtaaaa ataactttgg
    35521 aataatcatt gtaaaaagtc attggacctt catggaactt tgtatttatg acttgatgaa
    35581 gttttttggg ttgagaaaaa cttgaatcgg taaaagtgct gatataaata tcaaacggtg
    35641 cctggctaaa ctcattttca tcatcgtctt catactttat aaaaagggcc tgatctcttg
    35701 agcttaaaaa aatcaattca ttatcaacct caacggctcc aaactcggaa ttctccgaat
    35761 taacctgaag gtttgtcaaa agatacaaag atgaatctct ggtataaaaa tccagatcct
    35821 caaggaactt cagtttgtaa tcactccttg gatcatcggg aacatcctgc ttgtaatttt
    35881 cataccaggt tttagcttct tcgtaatttc ccagagccgt caaagcttgt gcataattat
    35941 acttatgaat gggcataagc gagggatttt caataattgg tctataccag gaaactactc
    36001 cccgcgggtt attaagtttg atgtaacact ctgcaatcct ggtttgagca ttaaggtctg
    36061 cgggattttt agccaatacc cgctcatata attctatggc attttcatat gccagttttt
    36121 cgtaataaat ttccgctttg cgtgaattac tcataaaaaa agttgtgata tcagcactct
    36181 gcgcatttgc agatgaaccc aatcctaaaa caagcaatat ggtaatgagc cgattcatta
    36241 gaaaaatcgg ggggaaatta atgcatcttt tggatacttg aattgataac taatcataac
    36301 ttcatgggat cccagatcca ccaggttaag ggcattggtg gttatggaat atgaataacc
    36361 aaatcgaagc tgccgtgtta cctgaacggc agccaaaata tctaccgcgc ttagaaatct
    36421 aaccgatgcc ccaacccaga taatatcatc aaaggaaaat tgagcattga gatcatactc
    36481 tactgcctcc cctccaacaa atttcaataa agtggaaggc ctgaatttta ccgccctgga
    36541 taaatcgaga gcatacccca cagtgagtat aatgggatta tcttgtttta ttgttgataa
    36601 gtctggactc ctggtgaaaa cattattggc aagttgcggc atgctgacac ctgccgtcaa
    36661 tcgatccgag ttgaaataaa ttccaaatcc gaagttaggc cgggttgacc gcacatcttg
    36721 ctgaaatgcc ggatcgttgg gattgaatat tgtagcccgg gtgtactcgg cgttaataga
    36781 atttattccc ccctggagcc ccaaggcaag atgccccttt tctgtaatct ttattcggta
    36841 ggcgtacata gcacttatgg agtactgatt aattacagat atgttatctc ttaccaataa
    36901 taaaccaatt ccaattttct tatttacaag gggagaatga atggccaatg actgagtatt
    36961 gggcgaaccg gttaatccaa ctgactggta gcgaacaagt gctgttgcgg tcaatactcc
    37021 attactgccg gcataagcgg gatttattgc cagaccatta aacatgtatt gactgaatgt
    37081 agcttgttgc tggctatatg caacctgtcc caaaaaaata aaaaaaagaa agagatattg
    37141 cctcattact ctttcttgag tattaaataa ccgcttaatg gtttgcttcc atctccaagg
    37201 ttaatcatat agaaataaga accttcagga acagatttat tagaaatgcc tttatttgat
    37261 tgtccaaacc atgctcgttc ctgattattg taggaattgg tttgaaaaac taaattatta
    37321 tacctgtcat aaattcgaat aaagttgttt ggaaataatt cgatcccttc aactctccag
    37381 gtatcattca ttccatctcc attgggagaa acagcttcat aaactataat ggggatgtcc
    37441 tccacaacaa tataaaccaa cgcgcctgcg caaagaggtt ccacatcatc atcacacaca
    37501 aggtaagtaa atgcatcaat accactatat gttggaaatg gtttataggt aaaactacca
    37561 tcaggactca atgtaacatt accatgaagc ggagcatttt caggatttgt attgataatt
    37621 agcacactgg actcaacatc ataatcgtta tctgttagat ttcctgaaac ggaactattt
    37681 cgttgaacca cgaccgtatc atttactgca actggtttat cgttgacagg attaattgtg
    37741 atttcaataa tggcaagatc gcaaagagtg ggcgtcccgt tatcacaaac actcaaggtt
    37801 gcatattcag ttccaaaaat attctcgttt ggggtgaaca gaaagcattc cgtagggccg
    37861 gtaccttcag tataactccc caatgaggtt tgagaattta ttgatatttc ctcttcgctc
    37921 tcaggatctg gatcctgaat tctgaaacaa aaatttatgg gaatgtcttc gttagtagca
    37981 agtctaagtg tatccaattc aagatcttca gaaataattt gtggaggatc attcactggc
    38041 aaaatattta tgacaacaac cgcggtatcg cataaggcgg gcacttgtgc gtcacatact
    38101 acgatctcac caaaggtagt accggtttca ttggtatctc ccgcaaagaa aaaacataaa
    38161 tctccaaggg gcaaatgttc aaatgcgcca ccgtaaccct caataggcga ggcatctacc
    38221 aagtcaacga aatcgccgtc ggggtcatca acatcaacac acagcttcgt tacaatatct
    38281 tccagaacgt caaaatatag agtatccacg ggaactcccc cgacggttac ctgcggaggg
    38341 tcgttaacgg gcaaaacggt acccacggca atgatagtgt cgcattgagt cgtgcttcca
    38401 acttcacaaa ctacgacttc caggatgtct gtaccattat agtcctgatt tggagaatag
    38461 gtaaagcaca agtttacagg atcgaaaata tcaatggtgg cattcattcc ggttgaatac
    38521 gcatctgcaa gctccagcgg atcattttcc ggatcgatag cttccaggca tatatcgata
    38581 ggttgatctt cgtttgtttc aaaaaacaaa gtatcgatag ggttgttatt ttccggatca
    38641 acaattacag gttcatcatt tacaggtata atttcaattg caaccacaat gtcatcgcac
    38701 agggatgggg tggcttcatc gcatacatct atgtcgatat actcaaatcc gttgtagttt
    38761 tccgctggag taaatgtgaa gcaaagccta tttaaaggat caaggtctac tgttccctga
    38821 ccgaattcga gcgcatatga aataatatca acggcatctc catcaggatc gaccacatcc
    38881 agacaaacat caatgggtat atcttccaaa gtagataccc ataatgtatc tataggatta
    38941 cccatatcgt ctactaccac cggtggactg ttggtggagg ccatgtcaat tccgcgggag
    39001 gtaatattac tcagatcgat gccatcattt acctggaaac ttatctggcg tgttaaaggg
    39061 tttacaagca ggattggatc aatgctggca ctgttcgaat attccaccga ctgcagtgct
    39121 gtctgataat taccaatgga agagacacct gtcagtgtca gtactccggt aacagcatca
    39181 aagtttcctg tgattccgtt ttgatcaaca aaaatcaatt gatcttcggt atcaatgtaa
    39241 ttgtttgaaa tggacacagt ggctccctgt agcgtggtat ggtcaatatc agtaatcaga
    39301 atcccattat cgacaataac attggcatct ccttcaaagt atgtgatctg gccggccgtg
    39361 cctgaaagta caggaggatc atttacatta ataacctgaa tgtctctggt caaaatattg
    39421 ctttcatcaa gtccgtcaaa aactttcacc gtaaaagtac gtgtggcaac tgacgggttt
    39481 tcactggaat tgctgtattg aattccattt attaccgatt gataaactgc caaagatgct
    39541 acacctgtaa tgtggacttc gccggttacc ggatcaaaac ttagtgtgag tccttgccct
    39601 ggtggcaggg taattatatc ctcactttgt atatagttaa ctgtaaaggt tatccatgcc
    39661 tcgtccattt gaaaatcatc gggatcgttg atggaaaggt caccaaacaa atttattggt
    39721 ggatcatttt ccgtataaac aagggcagta gtggaggcat taatagtggg aggtgtgttc
    39781 acaataacca gtgcaaagct aaggtccgaa tcagaaattc cggatgaaca ttgaccctca
    39841 agaagagccc ttgctgttat actatccccc aatgcgaggg gagtggcaaa agttgcctcc
    39901 cagattccgg atgtgcccga gcttgtagta atagattcaa ccagaacact gcttacatca
    39961 aaccggttga tgaatacatc tgcatttttt acggcagttc cggtgattat cgattcgcca
    40021 actttaattg gcgtatttat ggaaggtttc ggagttttac cgctcttgaa tccagcacag
    40081 gtctggcata agtcttcaag tgcggttgga cattggtttg aagttaaatc caaaaaagca
    40141 tcgctattat tagtataatc agcgtcattg acacctccaa tatccgagat cataccagcc
    40201 atcgcgcagg tagctgaaac atttgttaca gaggcgaaac gaagttcaga ttgcgcggtt
    40261 aatccaaaca gagtgttgag cgctgcaaac ggaatgtaaa aatcataaaa ataatccggg
    40321 tcatcgcagc tattcgtgcc ggcaatagcc ttttgataat gggtactgta gggaaaaaac
    40381 gctattggtg ccgggcaatc ctcaatacca tcaatatcgt aaatgtagac tccggcattt
    40441 gatcgtttta tgaaggtgat atcaatttca aaacctggat tttcgggggt tgcattgggg
    40501 tcgtcattcc caattttgtt atcggtatca atcagaatag tgtaagcttc tacacttgga
    40561 ttatcacctg ccagtctgaa tctgaaaatc aaattattat tttcaagaac tccataaaca
    40621 gaataccctg cggtatcgac tgccagatct gttgttccgc aaagtgcgcc agcttgtata
    40681 tcatttaata cttccccttc ctcataaatc ggaataccga acattttcaa ttcaaattca
    40741 tctacataat aaccatctat agaaaagccg gcggaggtaa gtgaaacaaa accatctcca
    40801 tttgaatcga tggtaccatc tcctgcctta aggatttgac ctcttaattg agaaaaagca
    40861 ttctcaataa agagtaaaag catgatactc agtaatatga gtctcgttag cttcataaag
    40921 ggtttagttt agctgctaag gtttcctaaa gataacacaa gtttccccac ccattaattc
    40981 caaaaacagg gccacctgta attacaaatg taagaaataa ggagaaacag tattatattt
    41041 taggcctgag gttactaaaa gttctcaaaa tacgcttgaa atagacattt gagcatattt
    41101 gccttccttt cttggagtaa ttttgaaatg taataaattg tatggtaggt aacatttttg
    41161 aacccctttg gcccaatttg ggaaaatcat gattaaaagt gggaaagtga tgggattttt
    41221 ttctatgcat caacatctac ctctaccata gttcgtgtga catcagcaag cgtttccctt
    41281 ttcctgatta ggtaatcctt tcctccagcg ataagtatct ccggtggtct taatctggaa
    41341 ttataatttg atgacatcga aaaaccataa gcacctgcat ttttgataag caaaagatcc
    41401 ccttcacgaa cttcgtgaag ctttctgtcc attccaaatg tatcagtttc gcagatgtag
    41461 ccaacaaccg tataaattct gttgattcct gttggattgg aaatattgac gatatcatgg
    41521 taagcttcat acatcatagg tcgtataaga tgattcatcc cggagtctac tccggcaaag
    41581 actgtagcag gtgttgtttt tacgacattc gttcttgcaa ttagcatccc ggcctcgctt
    41641 acaaggtatt ttccgggttc aaaccatatt tccagctcac gaccgtattc agagcagaat
    41701 tgtttaaaag cagttcccag atttttgccc aaatcaaaaa tattagtggt gttgtcacct
    41761 tcgcgatatt tcaccttaaa accgcttccg aaatcaatga atttcaagtc tccaaaatct
    41821 ctggcaattc caaaaagaat ctcggcgact ttcaaaaaaa cttcagagtc aagaatatca
    41881 gatccggtgt gcatatgcaa tccaataacc tgcagattgt gagtcttaat aattttcacc
    41941 aggtggggta actggtaaat gctaatccca aattttgaat ctacatggcc ggttgaaatc
    42001 ttggtgttgc ctccggcaaa tatgtgggga ttcagtcgca cgcaacaagg aacggaatta
    42061 tgatatgcat taccaaactg ttccaggatg gaaatgctgt caacattaac aagcagacct
    42121 aattcaactg cttcctgaat ttcatcaaag gaaacacaat tgggagtata gagaatttca
    42181 tgcggcttaa aacctgcctt taagccaatg tgcgcttcct ggatactaac tacatccaac
    42241 tcagccccca actgtcttat atattttaga atagaaacat ttgttaatgc ctttgcagca
    42301 tattttatcc gaaccttgtg ctccgaaaat gcgctcttta ttgccttgaa ttgactggat
    42361 atcttttctc catcataaac gtatagaggt gtaccatact tttcggccaa acctttcagc
    42421 tcaaggccct gaattgtgta aacgttattt ttaagttcca tgggtgtcta aaaaaatttc
    42481 acaaatatat aaagttgaat atggtaataa cagtaaattt catgttgtca actaatttcc
    42541 tggcggcgtt aaaatatgtt aattcagata attacctttc ttttcatcct acttttgcag
    42601 taagcatcta agtattcgga tgaaaaggaa gacgatcata tccataatca ttttgatgag
    42661 cctggcgcta gccggtctgg taagcttcca gttatattgg atcaatagcg taattaaatt
    42721 gaataacgaa aggttcatca gcaatgtcca tgaatcctta aatatggtga tttccaggtt
    42781 ggagaatacc gaaaatcttt tcttcgtgag ccgtggagta tcaagggttc tttttgagac
    42841 ggaagacgat aaaagtaagt tcacggactc ccttgccaac ttcaaagtaa tgattcatgc
    42901 ggacaccacc aagccaggag aaatcgcaaa aaaagagata gcttccctaa aggccaaaat
    42961 tcaaaatgat agtacagcca ggcaaagagt tcagaaagaa cttgaaaacg ggtggccacc
    43021 cgatcgcggc atcgtaagga aatccgggat ggttcaaata atattacagg aactcgcaag
    43081 tccaccaaag gttattcaac acagaataaa taaggaagtt ttagactctt tgcttcatca
    43141 ggaatttgca aacaaaggga tccatttgaa atttgagtat ggagtattga acccggtttc
    43201 agatgaaatc attcttgcaa ataccaaaaa tgtagaccat ttaagagaaa ccgatttgaa
    43261 agcgacacta ttccctaatg atattatttc caatgccaat tacctgacag ttaatttccc
    43321 tgataaaggg agttttcttt ttaaacaaat ctgggcaact cttgccagtt cattggtatt
    43381 gttgctggtg attgttggtt gttttgcctt tgcattgtta accattattc gtcagaagaa
    43441 attatctgaa atgaagaatg actttatcag caacatgacc catgaattca aaactccaat
    43501 tgctacagta tccttggctt gtcaagccct gggagaaccc gccattttaa accagccgaa
    43561 tatttttaaa aaatacctgg acatcataag agatgaaaat gaaagattgg aagatcaggt
    43621 tgagagagtt ttacacctgg cctctatgga gaaagaagat ttgaatctca aattcgataa
    43681 ggtaaatcta aatgagattc ttaaggatgt ttttcaacag atttatatcc aggttgaaca
    43741 aaaaaaagga agtattgttc tggaagaaaa gtcaccggaa atttgggtaa atggcgatgc
    43801 caaccatttg ttttccgttt ttttaaattt ggttgataac gcgaataaat attcacagga
    43861 taagccagac ataaaaatta tctgcgaaaa aaaaggagat aaggtaatgg tagaagttat
    43921 ggacaaaggg attggaatgt ccaaagaatc aatccggaaa atttttgaca agttctatcg
    43981 cgtacctaca gggaatgtgc acaacgttaa aggatttggt ctgggcctta attatgttaa
    44041 aaatattgtg gaagctcatg gcggcagagt atcagttcat tctgaaacgg gtaagggttc
    44101 ttcttttaaa gtagagttat gaatgaaaga aaaaaacttt tagtagtaga agatgatcca
    44161 aacctcggac aaattctgag ggagtatctt gaaattaaag gatatgatgc cgagctatgt
    44221 actgacggag aaaaaggcct ggccacttat agaaaaggaa agtttgacct ctgcatcctt
    44281 gacgtgatgt tgccaaagaa ggatggattt tcattggcaa gagaaatcag gattatggat
    44341 gaaaatactc ccatcatatt tctaactgca aaaaacctta aacaagatac cattgaaggg
    44401 tttaaaattg gtggagatga ctacatcaca aagccatttt caatggaaga attaattctc
    44461 aggttaaatg caatattgaa gcgaactact cccaatctta atggaagcgg gaatcaaaaa
    44521 ttcacttttg gctcatttga atttgattat ggccaatacg tgcttacatg caaaggcaag
    44581 tcatggagat taacagcaaa agaagctgat ttactaatgc tattgtgtca aaatataaac
    44641 aaacttcttg accggaaatt ggctcttaag cttatttggc acgacgacag ctactttaac
    44701 tcacggtcta tggatgtcta cataactaaa ctgaggaaat atttgaaaga agatgagcaa
    44761 cacgttcgca ttattaccgt tcatggacaa ggttttaagc ttgtatctat aaattaattt
    44821 ccctatattt cttatcggcc gcaagtttct ctttgaaact gaaccggagg cggcattctg
    44881 actcatttta ggcgatttct ctaaatagta gattagcaaa atcaataaag attatccaaa
    44941 gggtaatcca taaaattggt aacacaaatg atacaaatta ttccatccat agcgattcta
    45001 aatggcaaag tggtcaggtt gattcagggt aattatttcg ctgaaacgtc ctacaaggaa
    45061 agtccgctgg atgtagcaaa gaagtatgaa gcacatggaa tagaagtagt tcatttagtt
    45121 gatttggacg gtgcccagag aggatcccct gtcaattacc atattcttga ggccattgca
    45181 ggccatacat cgctgaaagt tgatttcaca ggtggaattc ataccgatgg cgacatcaac
    45241 aaagcgtatg aatacggtgc aacatttatt actgctgctt ccatagcagc aactaacccc
    45301 aatttgttcg gctcatggat aatcagctat ggcagagaaa gaattaccct gggagctgat
    45361 tccctggata gaaaaatagc catccgagga tggcaaaaaa ataccaccat tgatttgttt
    45421 gagcatattg attatttcta cagcaggggt ctcaaatatg taaaaaccac cgatatctca
    45481 agggacggag taatgcaagg gcccaacttt aaattatacc gtcagctaat gaaaacattt
    45541 ccaggcatga gtatacttgc aagcgggggt gttaaaaata tggatgatgt tgataagtta
    45601 aatgaaatcg gaatttttgc tgttattata ggaaaggcat tctacgaagg tcgtattaaa
    45661 ttgaaggaat tggaaaaata cctggttaca catccaatcc aaactttgaa atcctgatta
    45721 atctgatttt ctaaaaggat tcatccagca aatccgggaa cttaaatttc tcaatcaggt
    45781 aaaatagaaa attgaaagat gagactggcg aattattttg gtcctcaggt ggaacggata
    45841 attcaccggt gggtcgtttt tcttcgggtt taatttgctt gtttagaagt cctttgggtt
    45901 gctcagggat ttctgaatcc aaatctatct gagcagaatc aacctcggcg aggtcacttt
    45961 tttcgccggt ttgctggctt tcctgatttc cttctttatt attcttatct tccttaagag
    46021 atgtggcaaa ggaatttgta ccaatcagaa gacaggttat gaaactaacg ataaggagtg
    46081 gatttttcac aaaaaaaagc gctttctatg ctttaaacgt aatattgacc cattttgttt
    46141 caagttaata aaaaggaaaa tgtaacttag cgaggtcaca aaaattttta atgaatggcg
    46201 ttgatttcct gggaggattt tgagtcagta gaccttagag taggcactat tatttccgct
    46261 tcggagtttc ctgaggcaaa aaagcctgca tttaaactgg aggtggactt tggagcagca
    46321 ataggcgtta agaaatccag tgcacagata actaaacact ataatcccca aatacttctg
    46381 ggtaaacaag tgctttgcgt ggtaaacttc ccccccagac aaataggtcc ggttatttca
    46441 gaagttttag ttaccggttt tccggatgaa acgggcgaag taatattggc aataccggat
    46501 aaggaagttc cgaacggtaa aaaattattt taaccttgct gactacatta agcgaaattc
    46561 gttttctcgg tatcaaagat ttgccccaaa tgcatgaagc ttttttaaag gctttttcag
    46621 attacgagat tccattcaat ctttcgctgg cagaattcag caaaaaattt gttgaaaaat
    46681 taagtattga ctttggtctt tctccggcta tttttcatga aggtgagatt gccgcgttta
    46741 tatttacttc aatcaattat tatgacggaa gactaaccgc ttataatgga ggtaccggtg
    46801 ttttgcctgc atacaggggc agaaggtatg tgcaacaatt gtataacctt ctgcttccaa
    46861 agttgcagaa tagaatggtt taccactgtg tgttagaagt attaacaagg aatgaacggg
    46921 caatcaaggt ttatgagtca tgtggttttg gtaaatcaag gtattaccat tgttttaagt
    46981 tgttgggaag gaaaagattc aaaaattatc attatccatg gaatattcgg attagtacaa
    47041 cacctgactg gaaaacctat acgcggtatt ttgatttttt accatctttc ctggatactc
    47101 ccgcaatgat tgatcagaat ttaaagaatg aaaaaataat tgaggccata attcatggag
    47161 aagttgccgg atatgcaatt taccaaaagg cgcaaggaag aataagtcac atcggagtcg
    47221 catcaaattg gagaggcaaa ggaattgggt cgtcacttat tgattacatt tatgaggacg
    47281 ccgctaataa gaatcttacc ataataaata ttaacaaaga ggctgtaggt gtaaaagact
    47341 ttctaacccg catgggattt gaaaatcaat tcgatcaatg ggaaatggta aaaagtgttg
    47401 ataaatgatc acatttaact ctttgccagg catcactaat ggaaaaattg tcaatcaatt
    47461 aaaagattat gagattcgat atctggccat tgacagcaga aaaattattt catcccccca
    47521 cactttattt tttgccataa aaggagatcg gcatgatgga cacaattatt tggagtccct
    47581 ggtaaaggcc gggatcacca attttgtggt tgagacagac ctcccggttt caatggatat
    47641 ttcctcttgc aacattttaa aagtaaagtc ttctactgaa gcccttcaac atatagcagc
    47701 gaaccaccgt gcagaattta ccttacctgt aatcggaata acaggaagca gcggaaaaac
    47761 aattgtaaag gaatggctat tccagttgtt gcaaggcgaa agaattgtat ttaggagtcc
    47821 aaagagctat aactcacaag tgggggttcc cttatctgtt tggcaaatga acgctcacca
    47881 tgagttggcc attattgaag ctggaattag caaacccggc gaaatggaaa aactggaaag
    47941 gattattaaa ccggaaattg gcttatttac caatatcgga accgctcatg attcgggttt
    48001 tgcctcgaga gaagaaaaag cgcgcgaaaa aacaagactt ttcaaaaact gcaagttgat
    48061 aattttttgt agagatgatc tgcaagtgga gaacgcaata aaagaattgg ggagaaaaaa
    48121 aacgtttacc tggtcggcaa ccgacaaaga tgcggatttg caaattgtga agacaacatc
    48181 ccgcgaatta gtttgcagat ttaatgaaat tgaattcacc atcgaaattc ccggagacaa
    48241 caaagctagt attgataatt catctcattg cattgcagct ttgctctcca tgggaattcc
    48301 tgtaaaaaaa ataaaagaaa gtttcaaaaa tctgcaaccc gtttcaatgc ggttggaact
    48361 gaaaaaagga attaataact gcccaattat tgatgactct tataataatg acttgatggg
    48421 gttggaaatc gcgctggatt ttcttaccca tcaaagcaag aaagggaaaa aaaccgtgat
    48481 tctttctgat attctaacgt caggaattcc tgatgaagag ctttatcagc tggtagataa
    48541 acttttagat cagaagaaga tcagccggct gattggtgtt ggcccgggaa ttaattcaat
    48601 gaatgagaaa tttactctgc cgaggaaatt ttttgaaact acagaagaac tactcaaaga
    48661 actccccagg atatcatttg aaaatgaaac aatactggtg aaaggagcca gggattttaa
    48721 gctcgaaaaa gtcgtcgaca agctggtttt gaagattcac agcactgtat tggagatcaa
    48781 cctggatgcc ttaacccata atctcaatta ttacaagtcg aaactgaagc ctgcagtgga
    48841 aatcatggcg atggtgaaag ctttcgctta tggctcaggg agtgtggaaa tcgcaaggtg
    48901 gcttcaattt caccaggtaa attacctggc cgtagcttat gcggatgagg ggttaatact
    48961 acgtagccat ggtgtggaaa tacccatcat ggtcatgaac ccaactcctg attcatttga
    49021 taaattgatt tcctataatc tggaacctga gatatattct gagaaaattc ttaaaagcct
    49081 tttgaattat ttatcagaaa aagcagaaaa agtttctatt catttaaaga cagataccgg
    49141 tatgcaccgg ctgggttttg atgaagaaga attgaaccat gttaaagaac tgctacaaaa
    49201 tgaaaaaata accgttgagt cggtattcac ccacctggca gcacaggaaa atgcagatca
    49261 ggatgagttc accaggattc agattcagag ttttgaaaaa atgtatacgc aaatctccgg
    49321 ttggatagaa accaaaccca aacggcatgt tctgaattcg gcgggaatat tgcgatttcc
    49381 tgaatttcag tatgaacggg ttcggctggg aatcggtctc tatgggatag atccaattga
    49441 ggctggaaat gatctgatgc ctgttggaac atttcgaacc cttattagtc aggtgaaaaa
    49501 gataaaaccc ggggaaacga ttggctatgg aagaatagga aaagcggaac aaggtttgaa
    49561 gattgctact attccggtag gatacgcgga tggctttaac cgaagattca gtaatggagt
    49621 cgggcaggtt ataataaata ataaaagagc caaagtaatc ggtaatgttt gcatggatat
    49681 gaccatgatt gatattacag acattgatgc cagtgaaggc gatgaggtaa ttatctacgg
    49741 tgcagaaaat cccatagatg aaatggcaaa aaagattggt acaatatcgt atgaattatt
    49801 gaccgggatt ggagaaagag tgaaacgggt gtttgtccaa gagacgtaat ttgtctccat
    49861 cctcaacaaa ctgggcatta aatactatta attaaggaaa gcacataatg ggatgaaggc
    49921 gtacccggta actaccactc cgtgatgaag ttaaaagagt taaaaatttc ttttctgaat
    49981 gaggggattg gttaaaatct cactgaattt tcccactatt tatttcttta gaattattta
    50041 gaaaaagttg aagtatcaga aagaaatagc tttgcaaata ttatttattg atgaagctgt
    50101 gtattttcta atttttgata aatcttatcg gctttgtccc ggttgacccc ttaagatgca
    50161 ggatatacac tccttttttc caggagctca catccaattg ttcaaaacca ccttactgaa
    50221 actatctcca ccagttgtct ttattgcccg aactaacttt tgctaatcat ccgaaatttt
    50281 attcgaacgg ccatacataa ttcagcacca ggtaatacct ttgccgaaaa ttcaagattt
    50341 ttttcagccg ggtgatttta agcaaatcga tcatttggcc taatattttg ttttcgaaat
    50401 caaattgaaa aacacgactg cctgatgaaa tcttattcta gaattgccat attctttata
    50461 tttatggcat ttatcctggt tgcaggaaaa tctcaggcac agaataataa cacggtgagc
    50521 atgaattctt ccgagtatgc cattcggatt accattatca acggtgccac catgtctccg
    50581 gtcagaaatg cgactataac agtggatagc ctaacccgca cttacaagag tcaggtcgaa
    50641 ggagtctact ccattcgact tccacgggga aatcacacac taaagattgt cgcctcgggt
    50701 tatcaggacg tttatcagcc ggtttcagtt ttcgatgatg gccagttcaa attgaaaatg
    50761 tatcggctaa ttgaacgaaa agacagacta aatgtgggcc ggtatccggt taagctgagc
    50821 tttgataata tacaaaaaag gaaaggatta atgtttggat tcggattagg aaccggttac
    50881 tccaatcatg cggcatcgta taccgtaaga tctgatggct tcggtaattt taaagaattg
    50941 agcggctaca ggcttatgac tcttaatttc gaaataggat atgggctttc tcccaaattt
    51001 tttatcaatt ataactacaa atattctcca ccaaatacaa cagtttcggc atatcaaagt
    51061 acttatcacg gtggcgaatt gaacttttca cccggcagat ggggcagagg attaattact
    51121 ctgggtggag gatatcagat cgcgaaagat aaagaaggaa ccctggcgga tgggtattta
    51181 gttaatgccg gattagggta tgaagtttct ccccactttg tttttgaaat gaccaatttc
    51241 ctgggcacaa tgaataataa ggtagatgag aaaaccggaa acaggtttat gaatacttca
    51301 acggaattta ttctccagat ttcaatcaat tatttgttct attaatgtcc gcttcacttt
    51361 ggagaccact aatttattat gtgtgaattc aaaaaaaatt aatcacgttt ttggctcagc
    51421 cggaattatc cactaactca atattcagca cttcagcaag gctaattagt tcatctaaaa
    51481 tttcctcatg gttaggtgaa gaatccaata aataagtgga accatcctta agtaacagga
    51541 aggctttgaa ttcaaaaccc tgagacgtta atgtctgaga actgtgggtg ggagctgttc
    51601 ttttttggct aaactttact ttatttataa aaacttcttt tatgtggtga agaggaacgg
    51661 gtgcggcgat tccgatccca actgaaaata aaagcaaaga tttccttaat gtgatttgat
    51721 cgagatctac gaccacgacc ctttgtaaga caaatagtaa aaaagtaata ataattaaga
    51781 ttccaaaaaa tatctgggca ggcagactag tcagattatt ataataaatg taagctgtaa
    51841 aaatcaatgt cgctactaca aaccaggcgg aagctgaaaa ggaaacagct ctacgcttaa
    51901 agaattgatg cattcaggta agttagctct ctcctcagtt tgaagcaatg attaatattg
    51961 acaaatctga ttaaacacta tggaaacatg ataaaaggaa tatcggagta cgtaaggaat
    52021 atcgaagtac gtattaaaaa tgttcaatga ggtataaaat gaagtattta caacccggaa
    52081 atcgaatgta ttgaactcat tagttaattt aaagcttaat tttgtcgatc gtgaaaaagg
    52141 acgagataaa ggaaagtaat tttagtgaag aacatatata tatatcggta gaaacggaac
    52201 ttaaattgcg gcactcagaa ctcaacaagg gtgtgcgcca ctgcgagata attaattccg
    52261 aaaacaaagc agggaaagca acagtaaggg tggggaagca tttcatcgtg tacctgtttg
    52321 ttaacggaga tgcggatttg atatcttcca ttgatcagaa agttactcat ataagcaatg
    52381 accattatgc aatgatggtt tttccagtgt cggattggag ttttgaaata gagttacagc
    52441 cggagcaaaa tctaaagctt ttatgcattt caatttctga actccatgta atttttggaa
    52501 atctggatct tcaggatccc caaatgctga aagatgcctt aaagaattac aggcagaaat
    52561 cctttcttac cttaaagcct tccacaccgg ccgtcaggac aattattcac caacttttta
    52621 caataagggg tgaaggaata tccaggatgg tttatcaaaa gggcaagatt atggagttct
    52681 tatcagtctt tcttgggtcc agggataatg gaatttctga agccgaatgt ccttatatta
    52741 cagacagcct tgactgggaa aaaatacgca acgctgagaa gattatcaaa acagatctcg
    52801 cccatccgcc cacaataaaa gagttagcca aaaaggtagg aaccaatgaa ttcaaactaa
    52861 agatagggtt taaacacctg tataaaaata ccatctatgg ttacctgaat gattatcgca
    52921 tggattatgc gaaggaaatg ttgagcaaca agaatgtgct tattaaagat atttcagaaa
    52981 aagttgggta ttcaagtacc agtcatttta ttgcttcatt cagaaagaaa tttggcatca
    53041 ctccaaagca gtacctgaga ggatagaatt actattttgt cagcattatt ctaaccccga
    53101 tattttgata caatttgccc gtaagggttg tatcaaatcc aattaatggt tgaattctaa
    53161 acgctgaaat tcctgacgcg tcattggctg cattcatggc ctccactcca aaaagaatag
    53221 aggatgcaga acccaggatt gcgattgtaa gaccaaccgg cagaggtact tcaatttctg
    53281 aagcggaaag acctacggca actacgccat tcgccatggc aattccatac aatgcccagc
    53341 cggtaattat gattcccagc gaacttcggg atcctgattc ctttctcatc cttgaacctg
    53401 cagcggcgac aagaggaatc cctacacccc ctaagagcaa ggcggaagct cccaaaatgg
    53461 ttccggaagt aaaatcatct gataatcctg cagcggtagt gagtccgatt aaaggggccg
    53521 gaagtacaaa gccgatgatg gcaaaagctc gcgttcctcc gacactcgcc gttggcttta
    53581 ccagataata tttatcaaac tcaaaattgt atggcaactc gcttaatgat tgcgtagaat
    53641 tactatagaa ataggatgag cctgagcttg cagtttgcct gctaaaagca agaatcggga
    53701 tgaataaaaa caaagacacc aagcatattt gttttctcat ggggaagaga gaattacctg
    53761 aagccaggat gaatttagca ttttacccga ataaatccaa gacaaactca ggaagcaaca
    53821 tctttatgaa aaaattttta aaaagctttc gtggtgatat tcattatgct gcttttatct
    53881 ttatttataa tcattctaaa taataaaaac tctccggcag atcggcgcgg atttgcgcag
    53941 aaaaactctt ttctcccgca gatcgacgct gatttgcgca gaaatccctg aataaatttt
    54001 ctccgcggtg atccgcgata tcagcgggat taaagaaatc ttcttcagca gatcggcacg
    54061 cggatttacg ctgaaaaact tttctctcct gcagattacg ctgatttgcg cagaaatccc
    54121 tgaataaatt ttctctgcat cccggtttac cgggaccgac accacctcga atccagtagc
    54181 gttagttcct actttatcat acattcataa atgtatataa ttatctatat cctacgtata
    54241 gttatattaa tctcataatt aatttttaat atcatataat ttctctataa ttatcaatcc
    54301 tgagtttgct ggcttaatgt ttaacccttt aaaacttata aacatgagac gccaaactat
    54361 ctttttcatt tctttattca ttctccccgt tactcttccg gctcagaaca accatgatgt
    54421 gggcgaaccc gggcaaccca ttgaaaacat cattttgcct tacggcaggg tgggcattgg
    54481 tacactcact cccctttaca aactccatgt tgccggaaat ctgttcgttt cggatactac
    54541 ctgggcgaat aacctgatta ccgatcgcct cacttctgaa tttctaacca tcagtaagga
    54601 tgccactgtc aatagagact tgtttgtcaa cggcaaagtg ggcattggaa cactttctcc
    54661 gcaatacaaa cttgacctta ccggatcact ccgggccagt gaaacgctgt ttgccaataa
    54721 gctaattact tcatttgcag agtcgggtga tttaacgatc tccaatcagg caacccttca
    54781 caaagctttc attcaaaata ctctcggcat cggtgttgaa tcaccggctt acccattgga
    54841 tgttctgggt gatatccatg cctcctccgc tttgcggtca aattcggttg aaacctccca
    54901 gcttatggtg aactccggag cggcattctc tggaaatgta attatgaaca attacctggg
    54961 cattggaaaa tccaacccg
//
LOCUS       DASWHX010000159         5460 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_543851, whole genome shotgun sequence.
ACCESSION   DASWHX010000159 DASWHX010000000
VERSION     DASWHX010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 5460)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5460)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5460
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_543851"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(152..532)
                     /locus_tag="VGA21_10740"
     CDS             complement(152..532)
                     /locus_tag="VGA21_10740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651029.1"
                     /translation="MHLGIVHDDGPDQMGQMEKLSRYGKIYTFLHPGNGEENDNPLVE
                     FENGTKGIANRKYLRYDLVALDDDDEEEKGENQSKTSHPNLGNICGKIINPNDPSNPN
                     FKIIQDGLVCYYKWAGEEIAKLLS"
     gene            complement(595..1476)
                     /locus_tag="VGA21_10745"
     CDS             complement(595..1476)
                     /locus_tag="VGA21_10745"
                     /inference="COORDINATES: protein motif:HMM:NF024572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9651030.1"
                     /translation="MNKLFTDIEIIDRYLRDQSKGDEKDIVKIRLATDAKFRRLFDDI
                     ELMIQGIKETAIHTSREEKLIHLRNFYNEAYESSEDDILDDDDEEGGDEKTFGSSSED
                     YEEPTPGSGLGKSKVISFPAADVLRQYKVAVAAAVTLLIAAWYLIFQMNPITNEELFA
                     ENFVPFSNLDNNKRSTNEVLSERKQAYTAYDQGDYEQAALLFEKVITTSDNRLLDMFY
                     LGNAYLAIGNAENAIKNFKAVIKSETGLTTVAKWYLGLCYLKAEQTDLAREMLLEVQN
                     SGDERAEKAAGILRKLE"
     gene            complement(1490..2074)
                     /locus_tag="VGA21_10750"
     CDS             complement(1490..2074)
                     /locus_tag="VGA21_10750"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX9651031.1"
                     /translation="MNEKVIIEEIKKGNRTQLANIYRAYRSEFIAWLSSKFECSRDEA
                     RDIYQIAILTLHENIVNEKLKHLNSSIKTYLFAIGKNKILESKKAQNRFSYEIDPGEL
                     ELEEINNWEQEDREQHLELMKSSVEKLGNPCKSLLELYYYHNMSLEAIAGQMSYKNTA
                     TAKTLKFKCLGRLRNIFKVEMKNWISNNNNFLAQ"
     gene            2381..2989
                     /locus_tag="VGA21_10755"
     CDS             2381..2989
                     /locus_tag="VGA21_10755"
                     /inference="COORDINATES: protein motif:HMM:NF025092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEX9651032.1"
                     /translation="MMKKLSTIALMFIPALIFAQTKVEVNGEVKDKETGQTLSNCHVY
                     INEHFGTLTDENGVFKLEIPVEFENDNLHISYLGFETLDKPVKDLNEKFMKVDLVPGA
                     IWLDNVIVQADPWVDFRDIISEISSMYDNKQEFYAAVFNEIKNMEFPEPKNEAKNAEE
                     SLTSGEENKGEMLPGIIAFCIASVGLVFMARPFLRLINKDHA"
     gene            complement(3435..3812)
                     /locus_tag="VGA21_10760"
     CDS             complement(3435..3812)
                     /locus_tag="VGA21_10760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013455530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX9651033.1"
                     /translation="MDSLTPNIFVKNMNETIEFYRNIGFEVKMSVPEKGDFVWVMMSG
                     GNVNFMFQTFDSLGSDLPEISRQDGGSLLLYIQVKEIRQYHDRIKDKVNILKGLEKTF
                     YGATEFSIQDINGYILTFAENEE"
     gene            complement(3841..4197)
                     /locus_tag="VGA21_10765"
     CDS             complement(3841..4197)
                     /locus_tag="VGA21_10765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013455529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEX9651034.1"
                     /translation="MMRKSQDEEVQNLLNGIKMMDGEKYKSLVEIREIVFETYPETKE
                     KLMYGGIVFFLDNELFSGIFVNKKHLTFEFSNGYLMKDPYDRLEGKGKYRRHLKIKKR
                     EDITSKETAFFVKQAV"
     gene            complement(4410..4775)
                     /locus_tag="VGA21_10770"
     CDS             complement(4410..4775)
                     /locus_tag="VGA21_10770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008201674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HEX9651035.1"
                     /translation="MKTTPEHDERIAKMTFASVYPHYLTKVESKGRAKEELYQVIEWL
                     TGFNDKKLKELIDDKVTFERFFQKAKLNPNAHQITGVICGYRVQEITNPLTQKVRYLD
                     KLVDELAKGKKMNKILRQA"
     gene            complement(4777..5169)
                     /locus_tag="VGA21_10775"
     CDS             complement(4777..5169)
                     /locus_tag="VGA21_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009583718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEX9651036.1"
                     /translation="MKQDIQAYNDSQAASDKEICNLLSKTICNYLPEAENKIWHAHPV
                     WFLDGNPIVGYSKQKAGIRLMFWSGADFEEEALSVRGEKFKDASIFFNNVSEIEAKDL
                     KRWLKKSREIQWDYKNIVKRKGQLERLK"
     gene            complement(5193..>5460)
                     /locus_tag="VGA21_10780"
     CDS             complement(5193..>5460)
                     /locus_tag="VGA21_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012926883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4256 domain-containing protein"
                     /protein_id="HEX9651037.1"
                     /translation="DSAMGMAADMGIEVLSQEQYLELQKLGNPASPAGGFDAKTSSWL
                     KTPADIRKLGGALFADYRYGNVFVYHNGAESYYAARGFRGSVKV"
BASE COUNT         1505 a         1105 c          956 g         1894 t
ORIGIN      
        1 caggtatagt tggactcttg gcttgggtag aagaactaac acgttacgcg ttttacccat
       61 tattgaacct gagggagatt tctcccggga attattttat aaagtataat gtacctgact
      121 aattatactt taggtttatc aagttgatca cttaacttaa taatttcgcg atctcttccc
      181 ctgcccactt ataataacac acaaggccat cctgaataat tttaaagtta ggatttgagg
      241 gatcattcgg attgattatt ttgccacaaa tgtttcctaa atttggatgt gacgtttttg
      301 attggttttc ccccttttcc tcttcgtcgt cgtcatcaag cgctacgaga tcatatcgca
      361 aatattttcg attagcaata cccttagtac cattttcaaa ttctaccaag gggttgtcat
      421 tctcttcacc atttcccggg tgtaggaatg tgtaaatctt tccatatctg gaaagtttct
      481 ccatttggcc catttggtcc ggaccatcat cgtgaacaat tcctaaatgc atagttgtaa
      541 atatttaaat tgaacatggg tttagaatta tttcaatttt tattgaaatc gattttattc
      601 aagctttcgt aaaataccag ctgctttctc tgcacgctca tctccggaat tctgcacttc
      661 caacagcatt tccctggcaa gatctgtttg ttccgccttc agataacaaa gccccaggta
      721 ccacttggct acggtggtta accctgtttc acttttaatt accgctttga aattttttat
      781 agcattctct gcatttccaa ttgcgaggta cgcattcccc aggtagaaca tatccagaag
      841 ccggttatca ctcgtggtga tgaccttctc aaaaaggagc gcagcttgct cataatcccc
      901 ctggtcatag gccgtgtagg cctgcttcct ttcgctaagc acctcattgg tgcttctctt
      961 attgttatcc aggttactaa aaggaacaaa attttcagcg aataattctt cattggtgat
     1021 cgggttcatc tggaaaatca aataccaggc ggcaatcagc aaagtaaccg cggcagcgac
     1081 cgcaacctta tattgacgaa gtacatctgc cgcgggaaaa ctaattactt tgcttttacc
     1141 caatccacta ccaggagttg gctcttcata gtcttctgat gaagacccaa aagttttttc
     1201 atctccacct tcttcatcat cgtcatccaa aatatcgtcc tctgaagatt cataggcttc
     1261 gttataaaaa tttcttaaat gaatgagctt ctcttccctg gaagtatgta ttgcagtttc
     1321 ttttattccc tggatcatta attcaatgtc atcaaaaagc cggcgaaatt ttgcgtccgt
     1381 tgccagccgt attttcacaa tgtctttttc atcacctttg ctttgatctc tcaggtatct
     1441 atcaattatt tcgatgtcgg taaataattt gttcataatt tgttatttgt tattgggcta
     1501 aaaagttatt gttgtttgaa atccagtttt tcatttcgac cttaaatata tttcttaacc
     1561 ggccaagaca tttaaacttc agtgttttgg ctgtggctgt atttttataa ctcatttgtc
     1621 cggcaatggc ttccagtgac atgttatggt agtagtataa ctcaagcaga cttttgcacg
     1681 gattcccaag cttctccaca ctgctcttca tcaattccag gtgttgctct ctgtcttcct
     1741 gctcccaatt gttaatttct tctaactcca gctcgccagg atcgatctca taactgaacc
     1801 tgttttgagc ttttttggat tccaggattt tattttttcc tatggcgaac aaataggttt
     1861 tgatactgct attgaggtgc ttaagctttt cattaacaat attttcatga agcgtcaaaa
     1921 tagcaatctg gtaaatgtcg cgagcctcat cgcgtgaaca ttcgaacttt gaagaaagcc
     1981 aggcaataaa ttccgatctg taagcccggt aaatattggc aagctgggtc cgatttcctt
     2041 ttttaatttc ttcaatgatc accttttcat tcattatgat ctgggtttaa ttgaaaatga
     2101 tacaaaaggt aacccaataa attgctaaaa actcaactga ttccctgggt aatgggttaa
     2161 tgaaagttag cggaatttat ggggaaatgg aagcggagag gtattaatac agattttaaa
     2221 gactattgaa agttctttcc aaatggaaat actttcttat tggtaatgca ggagtagagt
     2281 aattacgggc atttggaggg ttacttattc gggtgatttc aattaatagc acgagtttct
     2341 ataataacga cacactgcaa tttcaaatct aaacgcccga atgatgaaaa agttaagtac
     2401 gatagcactg atgttcattc ccgctttaat ctttgcacaa accaaagttg aagtaaatgg
     2461 agaggtgaag gacaaagaaa caggacaaac tcttagcaat tgtcatgttt atatcaacga
     2521 acattttgga acactcacag atgaaaatgg agtttttaaa ctggaaattc cggttgaatt
     2581 tgaaaatgat aaccttcata tttcctattt aggttttgaa acattggaca agccggtaaa
     2641 agacctgaat gaaaagttca tgaaggtgga tttggtgcct ggagcgatct ggcttgataa
     2701 cgtaattgtg caagcggatc cctgggttga tttcagagat ataataagtg agatctcatc
     2761 gatgtatgac aacaagcaag agttttacgc ggctgtcttt aatgaaatta aaaatatgga
     2821 atttcctgag ccaaagaatg aagctaaaaa tgccgaagaa tctttgacaa gtggcgaaga
     2881 aaataaaggc gagatgcttc ccggaattat tgctttttgc attgcttctg tcggccttgt
     2941 ctttatggca agaccattcc tgaggttgat taataaggac catgcataac accatgcata
     3001 acaccatggt gaaagaaatc actcttggat ttctctttca ttcccgggaa gatttcagag
     3061 aagccgatta gtcgtatttt cataatttat tttcaaagta tattcaattt caacccctat
     3121 acttttgaag aatcaaatga gccggaatta taactcagtt tttttgttca atcctaataa
     3181 cgatagccgg ggttatagca atagtcagaa aagtcagctt tgctatttcc ctggcaattt
     3241 ttaacctggt acttgttcgg ttaatcttta aaagataatg acctggtggg aatggctgtc
     3301 agtggattca ttccatccac gcggttttta aatcctgaag ggattcttct tatcaattgg
     3361 ttttctatag atatttgaat ccttcagatt catttcaggc cggctttacg ctacaatgtt
     3421 ggcatttcgt tattttattc ttcattttca gcgaaggtca aaatgtaacc attaatatcc
     3481 tggattgaaa actccgttgc accgtaaaaa gtcttttcaa gtccttttag aatgttaacc
     3541 ttgtccttaa ttctgtcatg gtattgtctg atttctttta cttgaatata gagcaacaac
     3601 gaaccaccat cttgtcttga aatttctggc aggtcagaac ctaaactatc aaatgtctga
     3661 aacataaaat ttacatttcc gccagacatc ataacccata caaaatctcc tttttcagga
     3721 acagacatct taacttcgaa tcctatgttc ctatagaatt caatcgtctc attcatattc
     3781 ttcacaaaaa tgttcggtgt aagtgaatcc atatcttttt tgtcgtttat tgatttagat
     3841 ttatactgct tgctttacaa aaaatgctgt ttctttacta gtaatatctt ctcttttctt
     3901 tatttttaag tgtctgcgat atttaccttt accttcaaga cggtcgtaag ggtctttcat
     3961 aaggtatcca ttagagaact caaaagtgag atgtttttta tttacaaata tgccgctaaa
     4021 caattcatta tcgagaaaaa atacaatgcc accatacatt aatttttcct ttgtttcagg
     4081 ataggtctca aaaacaattt cacggatttc taccagggat ttatattttt ccccgtccat
     4141 catcttgatt ccgtttaata agttttgaac ttcttcatcc tgggattttc tcatcattta
     4201 ttttgcttat ctcctttgtg aattcttgct tataatgaat gcccaacggt tctgcttgtc
     4261 gttcgtgcgg aatttcggag tacaaaactg ttaaccagca ttaaaggtga tttgaaaaac
     4321 taaaattgaa tttaagcacg ttaactgcat aacgccaaac gcatgttata agctatgttt
     4381 ctttctgtgg tgtcgtttgt cattagtatt tatgcttgcc gcaaaatctt gttcatcttt
     4441 ttacctttcg ccaattcgtc cactaactta tctaaatatc ttaccttttg tgtcaacgga
     4501 tttgtgattt cttgcacccg gtagccacag attactcctg taatttggtg agcattagga
     4561 tttaatttag ctttctgaaa gaatctttca aacgttactt tatcatcaat caattctttc
     4621 agttttttat cattaaaacc ggtgagccac tctattactt gatacaattc ttcttttgct
     4681 ctgcctttac tctccacttt tgtgaggtag tgcggataaa ccgaagcaaa tgtcattttt
     4741 gctattcgct catcgtgctc gggtgttgtt ttcatgctat tttaatcttt ctaattgtcc
     4801 ttttcttttc actatgtttt tatagtccca ttgtatttct cgtgattttt tcaaccaacg
     4861 ttttaagtct ttggcttcaa tttcagaaac attgttaaag aaaatagaag catctttaaa
     4921 tttttctccc ctgacactta aagcctcctc ttcaaagtca gccccactcc aaaacattag
     4981 tctgataccc gctttttgtt tgctgtaacc aacaattgga ttgccgtcca aaaaccaaac
     5041 agggtgggcg tgccagattt tattttcagc ttccggtaag tagttgcaaa ttgtcttaga
     5101 aagcaagttg caaatttctt tgtccgaagc tgcttgcgag tcattgtatg cttgaatgtc
     5161 ttgtttcata tcttacaaac atattgttgt tattaaacct ttaccgagcc acgaaatccc
     5221 ctggctgcat aatacgattc tgcaccgttg tgatatacga agacattgcc gtagcggtaa
     5281 tcagcaaata gggcgccgcc gagttttcta atatcagccg gtgttttcaa ccagctcgat
     5341 gttttcgcat cgaacccgcc tgccggcgag gcaggatttc caagtttctg caactccaga
     5401 tactgttctt gtgataaaac ctcaatgccc atgtcagccg ccatgcccat tgcactatcc
//
LOCUS       DASWHX010000160        12313 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_3139012, whole genome shotgun sequence.
ACCESSION   DASWHX010000160 DASWHX010000000
VERSION     DASWHX010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 12313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12313
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3139012"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(59..1051)
                     /locus_tag="VGA21_10785"
     CDS             complement(59..1051)
                     /locus_tag="VGA21_10785"
                     /inference="COORDINATES: protein motif:HMM:NF005741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HEX9651038.1"
                     /translation="MDLSTKYMGLNLKSPLVVSASPLSEKLESIKLMEDSGASAVVLY
                     SLFEEQFEFEQHHLHHHTTAGTESFAESLSYFPEAEAYRTTPEDYLELILKAKMSVDI
                     PIIASLNGYSIGGWTHFAKMMSEANVDGIECNIYYIPSNLDMTGEDVENNYVEILQQV
                     REKVNVPVAVKLSPFFSNLANMAHKLDKAGANALVLFNRFYQPDIDLEHLDVLPHIVL
                     SKSYDMRLPLRWIGLLKGRIKASLAATSGIHTGWDVIKLMMVGADVAMLNSVLLKKGI
                     PEIRHIEETMVKWMEENEYKSIKQMQGSMSALNIKNPEKFERVQYMKALSTYCV"
     gene            complement(1056..4649)
                     /gene="nifJ"
                     /locus_tag="VGA21_10790"
     CDS             complement(1056..4649)
                     /gene="nifJ"
                     /locus_tag="VGA21_10790"
                     /inference="COORDINATES: protein motif:HMM:TIGR02176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HEX9651039.1"
                     /translation="MKNPKYVTLDGNEACSYVAYRVNEICAIYPITPSSSMAELADEW
                     SSKKITNIWGSVPTVYEMQSEAGAAGAVHGSLQSGALTTTFTASQGLLLMIPNMFKIA
                     GELTPAVFHVSARSIATHALSIFGDHSDVMATRTTGFALLASANVQEAMDMALIAQAA
                     TLESRIPFLHFFDGFRTSHEISKVELLKEDVMRKMINEKLIHDHRNRALSPDHPVIRG
                     TAQNPDVFFQNREAANSFFNNCPGIVQVKMDEFAKLTGRQYHLFDYYGNPEADRVVIA
                     IGSGTDTLKETVDYLNSHGGKVGLVIVRLYRPFDASALIASIPKSVKAIAVLDRTKEP
                     GSPGEPLYLDVTQAVMDDHFKTNPRFEEIPRLVGGRYGLSSKEFTPSMGIAVFENLKQ
                     GNHKNQFTIGIHDDVTGSSLNFDPDLCLEPDEVYRAVFYGLGADGTVGANKNSIKIIG
                     EQTGNYAQGYFVYDSKKSGSMTVSHLRFGPNKINSPYLIQKSNFIACHQPVFLERINV
                     IQSLMKSGIFLLNSPLHPEEVWQNLPKEIQKGLIEKNARLFTIDAQKVATETGSRRIN
                     TVMQACFFSISEVLPKEKAIAAIKDSIQKTYGKKGSRIVDMNLMAVDNSLNHLHEIPL
                     PKVPDSKFRIMDPVPENSPEFVKNVLGKIIGGAGDEVPVSAFPVDGTYPVGTAQYEKR
                     NIALKIPVWDEEICIQCGKCMMVCPHSVIRANVYDPNYLDSAPKGFKYTDSREKEWKE
                     KGLKYTIQVSPEDCTGCSVCVEVCPVKNKSNVSLKALNMADQAPLRLGEKANWEFFNM
                     IPDMDRSQIKITSIGQQQIQRPLFEFSGACSGCGETPYVKLLSQLFGDRAMVANATGC
                     SSIYGGNLPTTPWTKNALGFGPAWSNSLFEDNAEFGLGFRLALDKHEENARALIQESK
                     HLVGNDFAKEILEANQSDEQGINHQRERISKLKNILSKDKSEKGIRLLNIADYLVKKS
                     VWIVGGDGWAYDIGFGGLDHVLASGKNVNILVLDTEVYSNTGGQMSKSTPIGAVAKFA
                     ASGKPGKKKDLGMMAMSYGNVYVATVAFGAKDLQTLKAFIEAEQHDGPSIILAYSHCI
                     AHGINMEAPLQHQKALIDSGQWILYRYNPKLVEEGQNPLIIDSQPKKISIEEYMNLEN
                     RFRMLWKTHPEKAKEYYKQATESAKEKLAQLQYIASRPVNGQAKKLENVNP"
     gene            4826..7369
                     /locus_tag="VGA21_10795"
     CDS             4826..7369
                     /locus_tag="VGA21_10795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651040.1"
                     /translation="MTTNPGILTLTNFARVFAPGVVCWFLTGTLNAQQDTSRAASNLV
                     HRNLVTNEFIENTYHHYTLDHLVSNYYDFAYNQINTMADFSAPDPYVFSLVGHSFKWN
                     KYYYDGHRINDMYFPGTTLYKPFLYNKDVGFDLTSSEIEFHSKAGGKEEIILNYSGGR
                     LGEKVPWYEEYVNTLQGHSSAFPGAYQPIELRKHLNNSGSLYFFKNLEFNGKIIPFST
                     QATLGSRDQIRFDFTGISELYTERFGNILLSAKIPLRSDAPFDFLQSIVAFQQRDNLF
                     SEFYYDEMESSRLNQGSVSVWGTRNRKGRKLNLGLNLALHSIEKVNPSHSRNLIDQDG
                     EGMEPWYPSANVNQLNLNHQNTIQLNAKGFAFSIITNNGVIEHNPKERFYQNPVYLQT
                     VDTPFIPLYVTEWNSNKFTGALLENEANLNFNKTFSGGKFEFDGRGGFSFDGFLITSK
                     SYLNIAGQLRGRLSYKPNQNNKLNLDIGRGRIPFNFDHIRFLSEDYYTGQSYFWNDAN
                     GDQQFQSNEATGLYSTTGGGYHFLGSNLKQPSVYYIDLSWDWHLGKNWYFAINNEFRS
                     FRNLWTVEFDQDTASIGYFESFRGKSIFYFDGGNEINYRVTNFKPETLEINSDKKANF
                     PFDNPFYAGSTMNLSTEGEKLFLSVSFSAYMIVGYGPTGNGPLHNSPGELSESLANPN
                     NYINHVGRYDSDRAFIVRTLFGWKFSKSFSASLLILFKDGQPISTFETRLDSQIGRNQ
                     VAVWNYTNKGISPYNGQFGFREGAFWNYELKLKYDLQPRNVPFAINLYIYNLMDIATI
                     LNNYSFSPYTNYNPQFPFYNKQGYTPNRRSGMEVQIPRGFMIGLNYYFN"
     gene            complement(7362..8807)
                     /locus_tag="VGA21_10800"
     CDS             complement(7362..8807)
                     /locus_tag="VGA21_10800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009578663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HEX9651041.1"
                     /translation="MSPVLVFSVVGGYFILLITIAFFTGRQADSKTFFTANRQSPWYL
                     VAFGMIGASLSGVTFISVPGWVGTIQFSYFQMVLGYLLGYLVIATVLMPLYYKLNLIS
                     IYTYLEQRFGFWSYKTGAFFFLLSRVVGSSFRLFLVASVLQLAIFDAWGVPFELTVVI
                     TIILIWVYTFKGGIKTIVFTDTLQTLFMLLAVIISIKIITSELGISLSEAIGQIKEGP
                     HSQIFFWDQVDSKNFFKQFLSGAFIAIVMTGLDQDMMQKNLTCRNLRDAQKNMFWFSI
                     ILVLANVLFLALGAMLYIYSSSKGIDVPANTDDLFPSLAINHFSTFAGIVFLLGITAA
                     AYSSADSALTALTTSFCVDILNFKEGEDRRATRIKVHIGFSLILLLVIIIFKQINNQA
                     VISAVFTAAGYTYGPLLGLYAFGLFTRKKPVDKFVPWICVASPIISYVLNLNSEQLFM
                     GYKFGFEILILNGLITFLGLLLVSRKSNTFS"
     gene            8887..9168
                     /locus_tag="VGA21_10805"
     CDS             8887..9168
                     /locus_tag="VGA21_10805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017732777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HEX9651042.1"
                     /translation="MYNIFKHDEPQEVLEDVKVDDLKDLVVFNDDVNTFDHVISTLVK
                     VCKHSNEQAEQCTWIIHYKGKCAVKKGDEPFLRPMKEAILDAGINAAVL"
     gene            9177..9782
                     /gene="recR"
                     /locus_tag="VGA21_10810"
     CDS             9177..9782
                     /gene="recR"
                     /locus_tag="VGA21_10810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009186922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HEX9651043.1"
                     /translation="MQFPSKLIEDAVNEISKLPGIGKKTALRLALFLLKQKIEETESL
                     ANSLLNLRSRITYCGTCFMISDNTECTCRSIKKDHSTICVVEDTPDILAIENTSQYQG
                     MYHVLGGVISPIDGIGPDELQIESLIKRIQADPEIKEIILAISPTLEGDTTAFYISRK
                     LKGFGLKISTIARGIPVGGELEFTDEITLGRSILNRINYSE"
     gene            9855..11051
                     /locus_tag="VGA21_10815"
     CDS             9855..11051
                     /locus_tag="VGA21_10815"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018471982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEX9651044.1"
                     /translation="MMEKLSNRILALAESETLVMAQKAREFKERGVDIISLSLGEPDF
                     KTPKHICEAAKQAIDEGKYFTYPPVAGYTDLREAIAEKLRRDNNLDWAYQNIVVSTGA
                     KQSIANVFLSLLNPGDEVIILAPYWVTYSEIVKLAEGVPVFVKGNLNNNYKATADQIA
                     DAITSKTRMMIFSSPSNPTGSVFTEEELRQIADVLKDKNILVISDEIYEYINFTGKKF
                     SIGSIPEMKEKTITVNGFSKGFAMTGWRVGYIAAPLFIAKACNKMQGQITSATNSIAQ
                     RAALAALKSDFGPTKEMAAAYLKRRDLAYDLLNEIPGVKNYKPAGAFYFFPDFSSYFG
                     KSAGDRKISNSGDLVMYFLEEAHVSSVSGDAFGSPENIRLSYAASEAEIVEAIRRIKE
                     ALAKLS"
     gene            11062..12051
                     /locus_tag="VGA21_10820"
     CDS             11062..12051
                     /locus_tag="VGA21_10820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009578667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-heptose synthase"
                     /protein_id="HEX9651045.1"
                     /translation="MKLEEVFQKFSQMNFLVIGDLMVDCYIRGHIERVSVEAPIPIVK
                     VSSQDYHLGGAGNVALNLLALGAGSTICSVVGSDDDGRRLIHEIKELGLPENGILQSE
                     NRVSTVKTRVISGSQQVFRYDSEITDPIKAAEEKSLLEIIKTAMPNVEAVIFEDYDKG
                     TITKNIISETIGEAKKHNKPVIVDPKIKNFLNYQGVTLFKPNLKEIREGLRIDLPHYD
                     LQSIIKATNEVKSRLNCEGVLATLSARGIFIDYKEEKHHIPSHVRTIADISGAGDTVV
                     SVAALALVAGLSPKSIAALANLGGGIACESVGVNPISKEQLFEEAQQYLGEDI"
BASE COUNT         3700 a         2547 c         2425 g         3641 t
ORIGIN      
        1 cgcggaaaac agatatgtat taataaaaat gtgtttttac tcctatctct ttgcggcttc
       61 aaacacaata tgtacttagc gctttcatat actgaactct ctcaaacttt tcaggattct
      121 ttatattcaa tgcgctcatg cttccctgca tttgcttaat tgatttatat tcattttctt
      181 ccatccactt caccatcgtt tcctcaatat gtctgatctc aggaattcct tttttcaaca
      241 ataccgaatt caacatggca acgtcagcac ccaccatcat taacttaatc acatcccagc
      301 cggtgtgaat tccacttgtt gcagcaagac tcgctttaat tcttccttta agtaatccta
      361 tccagcgcaa tgggagtcgc atatcatatg atttactaag aacaatatgc ggcaatacgt
      421 ccaggtgctc aagatcgatg tcaggctgat aaaaacgatt aaacagtaca agagcatttg
      481 ctccagcttt atccagctta tgtgccatat tggcaaggtt agaaaagaac ggactaagtt
      541 taacagccac aggtacattt actttttctc ttacctgctg aagtatttct acgtagttat
      601 tctctacatc ctctcctgtc atgtcaagat tggagggaat gtaatagata ttgcattcta
      661 ttccatccac attagcttcg ctcatcattt tagcaaagtg agtccatccg ccgattgaat
      721 aaccatttaa actggcgatg ataggaatgt caacggacat tttagctttc aagatcaatt
      781 ccaggtaatc ctcaggggtg gtccgatatg cttcggcttc cggaaaataa ctcagagatt
      841 ctgcaaaact ttccgtgccc gcggtggtat gatgatgaag atgatgttgt tcgaactcaa
      901 attgttcttc aaataatgaa tacaaaacaa cggcagaggc accggaatct tccatcagtt
      961 ttatactttc cagtttttct gaaaggggtg aagctgatac cacgagtggg cttttcaggt
     1021 tcaaacccat gtattttgtg cttaagtcca tggttctagg gattaacgtt ttctaatttt
     1081 tttgcctggc catttacagg cctggaggct atgtattgaa gttgtgcaag tttctcttta
     1141 gcagattctg tggcctgctt ataatattct ttagcctttt caggatgggt tttccacaac
     1201 atcctgaacc tgttttcaag attcatgtat tcctcaattg aaattttctt tggctgagaa
     1261 tcaatgatca acggattttg tccttcctca acaagcttag gattatagcg atataagatc
     1321 cattgacctg agtcaattaa agctttctga tgttgcagtg gtgcttccat gttaattcca
     1381 tgtgcaatgc aatgtgaata agccaaaatg attgacggac catcatgctg ctctgcttct
     1441 ataaatgctt ttaaggtttg caaatcttta gcaccaaagg ccacagtggc aacatataca
     1501 tttccataag acatcgccat catacccaga tcctttttct tcccgggttt accgcttgcc
     1561 gcaaattttg ccacggcacc tatgggagtt gattttgaca tttggcctcc tgtgtttgaa
     1621 tacacttcag tatccaatac caggatatta acatttttgc cggaggcaag cacatgatct
     1681 agcccgccaa acccaatgtc atatgcccat ccatcaccac ccactatcca gacacttttc
     1741 ttaaccagat aatcagcaat atttaacaat cgtattccct tctccgactt atctttagaa
     1801 agaatatttt tgagcttact gattctttca cgttgatggt tgatgccctg ttcatccgat
     1861 tgatttgctt ccaggatctc ttttgcaaaa tcattgccga ctaaatgctt tgactcctga
     1921 attaaagccc gggcgttctc ctcatgctta tctaaagcta acctgaagcc caagccgaat
     1981 tctgcattat cttcaaaaag agaattagac caggccggtc caaatcctaa agcattctta
     2041 gtccagggag tggtgggtag atttccgccg taaatggatg agcatccggt agcattggct
     2101 accattgctc tgtcgccaaa taactgtgaa agtagtttta cgtaaggagt ctcgccgcac
     2161 cctgaacagg ctccgctaaa ttcaaacaaa ggtctttgaa tttgctgctg accaatggaa
     2221 gttatcttga tctggcttct gtccatgtca ggaatcatgt tgaaaaactc ccagtttgct
     2281 ttttcgccca gtcgcagtgg agcttgatcg gccatattca acgcttttaa acttacattt
     2341 gacttatttt ttacaggaca aacttccaca caaacactgc aacccgtgca gtcttcaggt
     2401 gaaacctgga tagtgtattt caaccctttt tctttccatt ccttttccct gctgtcggta
     2461 tacttaaagc ctttgggggc agaatccaga taattgggat cataaacgtt tgcgcggata
     2521 acagagtgcg ggcaaaccat catacatttt ccgcactgga tacaaatttc ttcatcccaa
     2581 accggaattt tcagagcaat atttctcttc tcatactggg cggttcctac cgggtatgtt
     2641 ccatccaccg ggaaagcact taccggaact tcatctccgg caccacctat gattttacct
     2701 agaacgttct tcacaaactc cggcgagttt tcaggaacgg gatccattat tctgaattta
     2761 gaatctggca ccttcggcaa gggaatttca tgcaggtgat tcagggaatt atcaacggcc
     2821 attaggttca tgtcaacaat ccgggaacct ttttttccgt atgttttttg gatagaatct
     2881 tttatcgctg caattgcctt ctcctttgga agaacttcag aaatactaaa gaaacaggcc
     2941 tgcatgacgg tatttattct gcggcttccc gtttctgtcg ctaccttttg ggcatcaatt
     3001 gtaaaaagtc ttgcattttt ttcaattaaa cctttttgaa tttcttttgg cagattttgc
     3061 caaacttcct ccggatgcaa tggtgaattc aataagaaaa ttccactctt cattaaggac
     3121 tgaataacat taattctttc aagaaagacc ggctgatgac aggcaataaa atttgacttt
     3181 tggattaggt aaggcgaatt gattttattc ggaccaaatc tcaaatgaga gaccgtcatt
     3241 gaaccggatt tctttgaatc gtacacaaaa tacccctgtg cataattccc ggtttgttcg
     3301 ccgataatct tgattgagtt tttatttgct ccaaccgtac cgtcggcacc cagtccataa
     3361 aaaaccgcac gatacacttc gtcaggctcc aaacataagt cgggatcaaa gttgagactc
     3421 gatccggtta cgtcatcatg aatcccaatt gtaaattgat ttttgtgatt tccctgtttc
     3481 aggttttcaa ataccgcaat ccccattgat ggagtaaatt cctttgacga caatccgtac
     3541 ctgccaccca ccaaccgtgg aatttcttcg aatcgcggat ttgtcttaaa atgatcatcc
     3601 ataacggcct gagttacatc aaggtaaaga ggttcacccg gagatccagg ctcttttgtt
     3661 ctatcaagaa ccgcaattgc tttaacagat ttgggaatgg aagcaattaa tgcggaagca
     3721 tcgaatggtc gatagagtct gacaataact aatcctactt ttcctccatg actatttaga
     3781 taatcaacag tttctttcag cgtatctgtt ccggaaccta tggcaattac caccctatct
     3841 gcttcggggt tgccgtaata gtcaaagagg tgatattgtc ttcctgtcag tttagcgaat
     3901 tcatccattt tcacttggac aatgccagga cagttgttaa agaatgaatt tgcagcttcc
     3961 ctgttttgga aaaacacatc cgggttttgc gcggttcccc gaattacagg atggtctgga
     4021 gataaagccc tgttccggtg gtcatgaatt aacttttcat taatcatttt tctcatcaca
     4081 tcttccttca gcagttcaac ttttgagatt tcatgagaag ttctgaatcc atcgaagaaa
     4141 tgcaaaaatg gaattctgga ttccaatgtt gctgcctgag caattaatgc catgtccatt
     4201 gcctcctgca catttgccga tgccagcagt gcaaatccag tcgtgcgggt tgccattaca
     4261 tcgctatgat ctccgaaaat agaaagggcg tgggttgcaa ttgatcgtgc tgacacatga
     4321 aagaccgcgg gcgtaagttc gccggctatt ttaaacatat tagggatcat gaggagcagc
     4381 ccctgagaag ctgtgaaagt tgtggttagt gcaccggatt gcaatgaacc gtggactgct
     4441 cctgcagcgc ctgcttcact ttgcatttca taaactgtag gtaccgatcc ccaaatattt
     4501 gtgatctttt tcgatgacca ttcatcggca agttccgcca ttgaggaaga aggcgttatt
     4561 ggataaatag cacaaatttc atttactctg taggcaacat aggaacaagc ttcgttacca
     4621 tccagcgtta cgtattttgg atttttcatc acttcacagc cttaaatttt gactttctcg
     4681 ggttgaaaaa gtacccacct tccctggcaa aaaaaatgac atgagtcagg caaaagaggc
     4741 acaattatca ggttatcaaa attttcttca tttacagaac tgtaatgact ttcatattct
     4801 gcaaacagag ccgatattcg caacaatgac cacaaatccc ggaatcttaa ctctcacaaa
     4861 ctttgcccgt gtgtttgcac ctggcgtggt ttgttggttc ttaaccggca cattgaatgc
     4921 ccagcaggat acttcccggg ctgcgagtaa tcttgttcac agaaaccttg taacgaatga
     4981 atttatagag aatacatacc atcattacac attggatcac ctggtgtcta actattatga
     5041 cttcgcctat aaccagataa atacgatggc tgatttcagc gcacccgatc cttatgtttt
     5101 ttcacttgtt ggtcattcgt ttaaatggaa caaatattat tatgatggcc accgcataaa
     5161 tgatatgtat ttcccgggca caactttata taagccgttc ctttataaca aagatgtggg
     5221 tttcgattta acttcatctg aaattgaatt tcattcaaaa gccggaggga aagaagaaat
     5281 aattttgaat tattcaggag gaaggcttgg agagaaagta ccctggtatg aggagtatgt
     5341 aaatactttg caaggtcaca gttccgcatt tcccggggct tatcaaccca ttgagttacg
     5401 caagcatctt aataattccg gcagtctcta ttttttcaaa aatttagaat ttaatggaaa
     5461 gataattccc ttttcaaccc aggctacttt gggttccagg gatcagatca ggtttgactt
     5521 caccggtata agtgaattgt atacggaaag atttggaaat attcttttat cagcaaagat
     5581 tcctttgcgt tcagatgcac cttttgattt tctgcagtcc atagttgcct ttcagcagcg
     5641 cgacaatttg ttttcagaat tttattatga tgaaatggaa tcttccagac tgaatcaagg
     5701 gtcagtctca gtctggggaa ccagaaacag aaaagggcgg aaattaaatc tcggattaaa
     5761 cctggctctt cattctattg aaaaagtgaa tcccagccac agccggaatt taattgatca
     5821 ggatggtgag ggaatggaac cctggtatcc ttcagctaat gtcaatcaat taaatctcaa
     5881 tcatcaaaat acaattcaac taaatgcaaa agggtttgct ttttcgataa tcacaaataa
     5941 cggagttatt gaacataacc caaaagagag attttatcag aatccggttt atcttcaaac
     6001 agttgacact cctttcattc ccctctatgt tactgaatgg aattcaaata aattcaccgg
     6061 cgctctcctg gaaaatgagg caaatctcaa tttcaataaa acatttagtg gaggaaaatt
     6121 tgagtttgat gggcggggtg gtttttcatt cgatggcttt ttaataacca gcaaatcata
     6181 tcttaatatt gccggccagt taaggggcag gcttagctat aagcccaatc aaaataacaa
     6241 acttaatctt gatatagggc gaggcaggat tccctttaac ttcgatcaca tccgattctt
     6301 atctgaagat tattacaccg ggcaatcata cttctggaat gacgcaaatg gagatcaaca
     6361 atttcaatct aatgaagcta caggattgta ttcaacaacc ggaggcgggt atcatttcct
     6421 gggttccaac ctcaagcaac catcggttta ctatatcgat ctttcctggg attggcattt
     6481 gggcaagaac tggtatttcg ctatcaacaa tgaatttcga tcatttcgaa acttatggac
     6541 ggtggagttt gatcaggata ccgcaagcat cggttatttt gagagttttc gtggcaaatc
     6601 aattttttat tttgacgggg gaaatgaaat caattacagg gtaaccaatt ttaagccgga
     6661 aaccttggaa attaattcag acaagaaagc caattttcct tttgataatc ccttttatgc
     6721 gggttcaacg atgaatctct caacagaagg tgaaaaatta tttctttccg tatctttttc
     6781 tgcttatatg atcgtgggct acggccctac cggcaatggt cctttgcata attctccggg
     6841 tgagctttcg gaaagtttag caaatcctaa taattacatc aatcatgtgg ggcgatatga
     6901 ttcggatcgg gctttcatag ttcgcactct ttttggatgg aagttttcaa aatcattttc
     6961 ggcaagtttg ctgatacttt ttaaagacgg ccaacctatc agcacatttg agacccggct
     7021 ggattcccaa attggcagga atcaggtagc agtgtggaac tacaccaata agggaatcag
     7081 cccttataac ggacaatttg gttttagaga gggtgctttc tggaactatg aattgaaatt
     7141 gaaatatgat ttgcagccac gcaatgttcc tttcgcgatt aatttgtata tctataatct
     7201 catggacatc gcaacaatac tcaataatta ttctttctca ccctatacaa attacaatcc
     7261 tcagtttcct ttttataata agcagggtta cacacctaac cgtagatcag gcatggaagt
     7321 acaaataccc aggggattta tgattggact taattattat tttaactgaa ggtattggat
     7381 ttcctgctta ccagcaacaa ccccagaaaa gtgatcaaac catttaaaat aagaatttca
     7441 aaaccaaatt tatatcccat aaacaactgt tcggaattaa gattcaggac gtaggaaatt
     7501 atgggtgaag ctacgcaaat ccagggtaca aatttatcaa ccggtttttt ccgggtaaac
     7561 aatccgaagg catacagccc aagcaaaggt ccatacgtat aacctgccgc cgtaaaaacc
     7621 gcacttatta ctgcctgatt atttatttgt ttgaagatta taatcaccag cagcaggatg
     7681 agtgaaaatc caatgtgtac tttgattctg gtagcgcgcc tgtcttctcc ttccttaaaa
     7741 ttcagtatat cgacacaaaa ggaagttgtt aaagcagtta aagcggagtc cgcgcttgaa
     7801 taagcagccg cagttatccc cagtagaaat acaattcccg caaaagtact gaaatgatta
     7861 atagcaagcg aaggaaacag gtcatctgta ttcgcgggaa catcaattcc ttttgatgat
     7921 gaataaatgt aaagcatagc tcccaaagcg agaaacagta cattggcaag aacaagaatg
     7981 atggaaaacc aaaacatatt tttctgagcg tcacgaaggt ttctacaggt tagatttttt
     8041 tgcatcatat cctggtcaag tcccgtcatc acaatcgcaa taaacgcacc gctcagaaat
     8101 tgtttaaaaa aattcttgct atcgacctgg tcccaaaaga atatttgaga atgagggcct
     8161 tcttttatct ggccaatcgc ttcactcaat gaaattccaa gttcagaggt tattatttta
     8221 atagaaatta taactgccaa aagcataaac agggtttgaa gagtatcagt aaaaacgata
     8281 gtctttattc cgcctttgaa ggtatatacc cagatcaaaa taatagtgat gacaacagtg
     8341 agttcaaaag gaacacccca ggcatcaaaa atagcaagct gtaaaacgct tgccactaaa
     8401 aacaatcgga atgacgatcc tactactcgt gaaagcagga aaaaaaatgc accggttttg
     8461 tatgaccaaa atccaaagcg ttgttccagg taggtataaa tggagatcag atttaacttg
     8521 tagtaaagcg gcattaacac agttgctatc accaggtaac caagcaggta tcccagcacc
     8581 atttgaaaat aagaaaattg aattgttccc acccaccccg gaacgctgat aaatgtaact
     8641 cccgacagag aggctccaat cattccgaag gcaacaagat accacggcga ttgccggttc
     8701 gctgtgaaaa aggtttttga atctgcctgc cttccggtaa aaaaagctat tgttatcagc
     8761 aaaataaagt agcccccgac tacgctaaat actaaaaccg gactcataac aggatttttg
     8821 aatgcgataa tactttaatt tttattactt ttgcaacaaa ccctggcagt aaagcagtaa
     8881 acaattatgt acaatatctt taagcatgat gaaccgcagg aggttttgga agatgtaaag
     8941 gtagatgatc tgaaagatct tgtggtcttt aatgatgatg taaatacttt cgatcacgtg
     9001 atcagcacac tagttaaagt ctgtaagcac tctaatgaac aagctgagca gtgtacgtgg
     9061 atcattcatt acaaaggaaa gtgtgctgta aaaaaaggcg atgagccatt tcttcgaccc
     9121 atgaaggaag ctatcctcga tgcgggaata aatgcggctg ttctttagaa atacccatgc
     9181 aatttccttc caaactaatt gaagatgctg taaatgaaat ttcaaaactt ccgggtattg
     9241 gaaaaaaaac cgcattgcga ttggcattat ttctactgaa acagaaaata gaagaaacag
     9301 aaagtctggc aaattcactt cttaatcttc gtagtagaat tacgtattgt ggtacatgtt
     9361 ttatgatttc agataacacc gaatgcacat gccgttccat taaaaaagac cactccacta
     9421 tttgtgttgt ggaagataca cctgacattc tcgccatcga aaatacttcg caataccagg
     9481 gaatgtatca tgtactcgga ggagttattt caccaattga cggcattgga cccgatgaac
     9541 ttcagattga atccttaata aagcggatcc aggccgaccc ggagattaag gaaatcatcc
     9601 tggccataag tcccactttg gaaggagata caaccgcatt ctatatttca agaaaattga
     9661 aaggctttgg tcttaaaatt tctacgattg cccgggggat acctgtcgga ggtgaactcg
     9721 aatttacgga tgaaattacc ctgggaagaa gtattctgaa ccgaattaat tattcagagt
     9781 aagcagtaaa tcacaaaatg cctccatata tttcatccga atacaaaaac actatttttg
     9841 cttcatataa ttaattgatg gaaaaacttt caaaccgaat tttggcgcta gctgaatccg
     9901 aaacgctggt aatggctcaa aaagccagag aatttaagga aagaggagtt gatatcatca
     9961 gccttagcct tggtgaacca gattttaaaa ctccaaaaca tatttgtgaa gctgccaagc
    10021 aggctataga tgaaggtaaa tactttactt atccccccgt ggccgggtac acagatttaa
    10081 gggaagctat cgcggagaaa ttgcgccgcg ataataatct tgattgggct taccaaaaca
    10141 tagttgtttc aaccggtgcc aagcaatcta tcgccaatgt ttttttaagt ttgctaaacc
    10201 cgggagatga agtaatcatt cttgcaccct attgggtaac ctacagtgaa atcgtgaaac
    10261 ttgctgaagg tgttcctgtt tttgtaaaag ggaatcttaa caacaactac aaagcaactg
    10321 ccgaccaaat tgcagatgct attacctcta aaacccggat gatgattttt tcatctccaa
    10381 gtaatccaac cgggtctgtt tttactgaag aagaactcag acaaatcgcg gatgttctaa
    10441 aagacaaaaa cattctggtg atctccgatg agatctatga gtatatcaac tttaccggaa
    10501 aaaaattttc tattggctcg attccggaga tgaaggaaaa aaccataacc gtaaacggat
    10561 tctcgaaagg ttttgcgatg accggctggc gtgtaggata cattgcagct ccattgttta
    10621 tagcgaaagc atgtaataag atgcaaggcc agattacatc ggctacaaac tcaattgcac
    10681 aacgcgccgc cctggctgct ttgaagtccg attttggtcc caccaaagaa atggcggcag
    10741 cgtatttaaa gcgccgggat ctggcttatg atcttttgaa tgaaattcca ggcgttaaaa
    10801 actataaacc tgccggcgca ttttatttct tccctgattt tagcagttat tttggaaaat
    10861 cggctggaga tcggaaaatt tcaaactccg gtgacctggt aatgtatttt ctggaagaag
    10921 cccatgtgtc ttctgtaagc ggggatgcgt ttggctcgcc cgaaaacatc cgattatctt
    10981 atgcggccag tgaagcagaa attgtcgagg caattcgaag aattaaagag gctttagcaa
    11041 aactttcgta aattaaatcc aatgaaactc gaagaggtct tccagaaatt ttcacaaatg
    11101 aatttccttg taattggaga cctcatggtt gattgctaca tccgcggtca tattgaaaga
    11161 gtttcggtag aagcacccat acccatagta aaagttagca gtcaggatta ccaccttgga
    11221 ggtgccggca atgtggcact aaatctattg gcacttggtg ccgggtcaac aatttgttcc
    11281 gtggtgggtt cagatgatga tggcagaaga cttattcatg agattaaaga attaggatta
    11341 cctgagaatg gaattttaca atctgaaaac agagtctcaa cagtaaaaac gagagtcata
    11401 tccggatcgc agcaggtttt tcgatatgat tccgagataa ccgatccgat aaaagccgcg
    11461 gaagagaaga gtctgctgga gataattaag actgcaatgc ccaatgtgga ggcggtaatt
    11521 tttgaagact atgacaaggg tactattact aagaatataa taagtgaaac tattggtgaa
    11581 gccaaaaaac ataacaagcc cgtaattgtt gatccaaaga ttaagaactt cctgaactat
    11641 cagggtgtaa cgttattcaa accgaatctc aaggaaatcc gggaaggact tagaatcgat
    11701 ctgcctcatt acgacttgca atcgataatt aaagccacta acgaagtaaa aagcaggctt
    11761 aattgcgagg gagtattagc caccctttcg gccaggggca tctttattga ttataaagag
    11821 gaaaagcatc atataccctc acacgtaaga acaattgcgg acatttcagg agctggcgat
    11881 actgtagtaa gcgttgccgc cttagcattg gtagcgggat taagcccaaa gtcaatcgct
    11941 gctttagcaa atctgggcgg tgggattgcc tgcgagtcag ttggcgtcaa tcctatcagc
    12001 aaagagcaac tttttgaaga agcgcagcaa tatctgggtg aagatattta aggttgcttt
    12061 tgtactttgt attccagcgt ttttaatgcc tgtagtttat actgaaacca aagttcaaag
    12121 gcagatatga gagaaatttg tctgtatttg taaatttttt tatccaaaca cccccccagt
    12181 ttttaccaaa tttaaagtgt tcccattatt aagattgttt tccatgtcag tgacaattaa
    12241 tcgatgaagt caagttctga ggttaattat tttttgagtt tctacaagtt attatatagc
    12301 ttttataatt tat
//
LOCUS       DASWHX010000161        13800 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_3140905, whole genome shotgun sequence.
ACCESSION   DASWHX010000161 DASWHX010000000
VERSION     DASWHX010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 13800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13800
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3140905"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(25..1464)
                     /locus_tag="VGA21_10825"
     CDS             complement(25..1464)
                     /locus_tag="VGA21_10825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009577685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX9651046.1"
                     /translation="MNLPEKITELEKSAIHLEPDVEERKEIRNWMVNYTEDFLNSIRD
                     LKTYEIEDAGWKNDPFQESGINDFKELDPYLRSVDNSGINPASGGHLGYIPGGGLYTS
                     SIGDYWAAITNRYSGILFANPNAVRMENQLIRWMAQLAGYPKGCAGNLASGGSIANLS
                     AIVTARKASKVKAADFPKLVIYLSPQTHHCVDKAIVIAGLEESVLRRLPLNSLHQIDA
                     DKFEVLVEKDIADGLKPFLLVASAGTTDTGAIDPLSRLGDICETNNIWFHVDAAYGGF
                     FLMCDSVKSKFRGIEKSDSLVMDPHKGLFLPYGLGVVLVKDGQMMKEAFKYFANYMQD
                     AHVVSEDVSPADVSPELTKHFRGPRLWLPLKIHGLKAFRDCLEEKILLTNYFYQELQK
                     INGFEVGPEPELSVCIYRYRPSSGDINEFNRSLVKEMHKDGRVFISSTTINGEVFLRL
                     AVLSFRTHLREIDLALSTLKEKIRILESK"
     gene            1543..2100
                     /locus_tag="VGA21_10830"
     CDS             1543..2100
                     /locus_tag="VGA21_10830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020527304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEX9651047.1"
                     /translation="MSLTPSKMVPLGTEAPDFILPDTVSGQQMSLNQLKSEFATVIMF
                     ICNHCPYVKHVREELVRITNDYTKRAVSFIGISSNDIKEYPEDSPGKMKEEAIKYGYS
                     FPYLFDETQEVARDYGAACTPDFYLFDKRMKLVYRGQLDGSRPGNETPLTGTDLRNAI
                     DAVLSGNPVSDLQIPSVGCNIKWKD"
     gene            complement(2553..3092)
                     /locus_tag="VGA21_10835"
     CDS             complement(2553..3092)
                     /locus_tag="VGA21_10835"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX9651048.1"
                     /translation="MEYLTKINEISEQARQEFSSLTIDQLNWKPHPDKWSIGQCLDHI
                     ITTNSTYLQVFESIINRKYKSDLWSRLSPFSNYFGNFMIKTLGPERRKVYRSPGVFRP
                     SQSEITPDITLRFIKHNNELISRSNQIRESGFEDIVIHSPASFFVTYKVSHAIAIIAR
                     HEERHLLQAFDVKGLLMSS"
     gene            complement(3098..4060)
                     /locus_tag="VGA21_10840"
     CDS             complement(3098..4060)
                     /locus_tag="VGA21_10840"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX9651049.1"
                     /translation="MRRFVHNIWLLALIMSLLAACGKPSKNTEQDENPVENFSGLVSI
                     NGTELYCKIIGSGEPVVILHGGPGLDHTYLLPHFLALADKYQLIFYDQRGGGQSSINV
                     DTASISMENFIEDLEGIRKHFEVGKISLMAHSFGGLIAMNYAIKYPENLNSLILIGSV
                     APTSEMMQSASLLDAANVSPEDSIEFNEILTSEEMANGEAAAIEKLMRLSFKRQFFNK
                     NFADSLNLSITERFITTSGLLSGLQKDLIDFDLTDELAQVTVPTLIIYGDYETTYKVS
                     DEILYKTMPNGQNWVLRDCGHFPFIEKKNDFERIVNNFMAGLKE"
     gene            4180..4569
                     /locus_tag="VGA21_10845"
     CDS             4180..4569
                     /locus_tag="VGA21_10845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651050.1"
                     /translation="MVFSRPGSLLVSLIEVEEQDFDPSSFTFKATYKLKFYNNNLLLR
                     KRIKYPSICINKNGKILECPLSDLNGDEIDVINIPAKKSETIRATVNESNPPFTDIGE
                     FTYNLSYYQHGSKKPVKVLISANNSRQ"
     gene            4691..5251
                     /locus_tag="VGA21_10850"
     CDS             4691..5251
                     /locus_tag="VGA21_10850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009583310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX9651051.1"
                     /translation="MDSLTDNALMMQVKNGDLDKLGLLFERYNRILFAFFYRLGNDSE
                     VCEDLIQNVFMRIVKYKKNFKGEGQFKHWIFHIARNIHADNYRVNNKLGKRESLENYS
                     DRLTDHGELVTKDEVDDNVSLLKKCISMMDEEKRELITLSKLEGMKYKDIGTIINCSE
                     GTVKVKIFRALKELKDIFQKTKLNHG"
     gene            5244..6041
                     /locus_tag="VGA21_10855"
     CDS             5244..6041
                     /locus_tag="VGA21_10855"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX9651052.1"
                     /translation="MDKERQKQLIAKFNNLEILPEELILLEKAIEIGEIGLQELKEIS
                     DLDRQLDFRIPEPGENLRAAFYANLAKLKASKSRGNAITGLNIWLTNLGFSQSAIRLA
                     YAFVLVIVGFGIGQWTQSSIPYEKIDALTTQVSEMKGLMMLTLIKEPIATDRLKAVNL
                     AMDADKVDERMILALLNTLNNDDNVNVRLASVEVLLRYSNNPVVRQGLVEAIPNQDNA
                     TMQYALAEAMVLMQEKNSIERLQELMQKEDLDEDVRNKIHESIQKLS"
     gene            6043..6888
                     /locus_tag="VGA21_10860"
     CDS             6043..6888
                     /locus_tag="VGA21_10860"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HEX9651053.1"
                     /translation="MKTLIIISTFCFASIAANGQERKQTINKEYDLAGPAKVLRISNV
                     NGFIKVEGYTGSKVTMEINESLKAKTQSSLEDMITKLSIGTEEYGDTLEIFVDGMCKC
                     DCERGRRYNWNNCDFKGSFLYDFAVRVPQNLNVILSTVNKGDIEVKNVSGKIQANNVN
                     GAIYLNDVREALKVRTINGDVEIHYTDLPKENSEYYTLNGDLTVTYPSALSADMSFKS
                     FNGEFYTNFSIKERLPAIATTTERGGHGTTYKIDETTSVRVGNGGVKLDFETFNGDIF
                     IKKNQ"
     gene            6931..7734
                     /locus_tag="VGA21_10865"
     CDS             6931..7734
                     /locus_tag="VGA21_10865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651054.1"
                     /translation="MKKQMIKLVGVFILIIVFQAKAQEYDQIVIPLSSPDKTGALKLS
                     LPNGTIKVTGYSGKDVILKVSTEQQKINKGTKDGLMKIPNRSLGITAKESENLVTVSG
                     GYNRDVIMEIQVPTKFDLKLTGHNGGDFTVENINGNVEITNHNAGIAARNISGAAILN
                     SHNGDITATFVSVTPDTPMAFTTFNGDVDITFPPTVKATFKMRSERNDILTDFELNLK
                     EAKTKESGRDEDGVYKISIEEWTVGDVNGGGPEMLFKTYNGDVIIRKKK"
     gene            7737..8315
                     /locus_tag="VGA21_10870"
     CDS             7737..8315
                     /locus_tag="VGA21_10870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651055.1"
                     /translation="MRKLTSALVILLLGATAFAQSSRYEDLEYFDKVILKGNVKKVSL
                     ENGDREIVVDGADKNSVKLSRLAGIITLEITSSGPVAVSITNGSLKWIEASSETEIEG
                     AEYLSGGKGKYLVVGMKDSKGRSNNENFNFNFDDFDFDFDYDFDSDFDFDFDFDYDFD
                     FNFDRDYSYNYDYKYDYKYDRKVNRVKEKNDN"
     gene            8541..10970
                     /locus_tag="VGA21_10875"
     CDS             8541..10970
                     /locus_tag="VGA21_10875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009583304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9651056.1"
                     /translation="MLKNYITIALRNITRQKVYTFINVSGLAIGIAACILILLFVKDE
                     LSFDQYHEKSERIYRVSREWMNKDGETSLHLGHVAPPFGPLLENDFSGMIEYAVRFQS
                     GYSPLVKANGKDIQEERFFFGDQNIFKVFSWKVLDGDPETALLEPNAVVLTKTTAEKY
                     FGSENVVGKQITYNNFGLSIEMKVTGVMEDVPLNSHFKFDFLGSFKTIENVFGADNLA
                     RSWGSNNYATYLLLGEGTAPEQLTAEFPGFLNNHMGPLDDGRNRSDVNRLHLWPLASI
                     HLYSHLDSEVEPNGDIAYVYIYSIVALFILVIACINFMNLSTARSAKRAQEVGLRKVL
                     GAVKGLLMRQFILESVIFSIISLLFAVLFVLMILPFFNDFVQKDLSLNVMENPFVALL
                     LVGITLLVGLVAGSYPAFYLASFQPATILKGSHKASGKKISLRSVLVIFQFWISIVLI
                     IGVGIVQDQLEYMRKKDLGFNSDNMLVLPPSNEIYNNFESVKQRLENNPAIEQVTISS
                     RVPSGRLLDSQGGEAEINGEMVDINVRVADVHVDHEYLEQLEIPLAAGRYFDRSRASD
                     SSLAFIINEATVRALGWDSSEEAIGKKFNYGSRQGGEIIGVVKDFHFESLHQPIVPIV
                     FLVTQGRFSNVIVKVKKGMEQEALAFLQDQWQFLRPGFPFTSFYIEEQFSNQYGNEDR
                     LHKIVKYFSGLAVLIAALGLFGLASYTAEQRIKEIGIRKVMGASVPQILILLTSSFTR
                     LVLIAFILAIPLAWFGMDAWLSNFAFRGQLQVMSFAIGGVLALLIAWITVISQTLKAA
                     MTNPVDSIRYE"
     gene            11085..13487
                     /locus_tag="VGA21_10880"
     CDS             11085..13487
                     /locus_tag="VGA21_10880"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9651057.1"
                     /translation="MEFLRENLITSVRSILRNKFFALLNILGLTIGITSFIIISLYVW
                     DEITYEQWHANKDTIYRLEVEYFLPNDGGSQYMAVTAPIISEIVQKDYPEVEYRVRIQ
                     EWRDELIENYAGEKYYESLYFADSAFFQMFSFELNARVNEMPLSEPYSAVITEDLARK
                     YFGRTDVLGELLRLKSFDKDVRITGILKSYESNTHLRPEFLVSMATLKSENELPQHWW
                     TFNTYSYLKLITNSDANALASKIKNISGRYIREQEVSSGYHQEYSLKNIKDIHLNSHI
                     RGEISSNNKASFVFLFGAIGIFIIVIACINFMNLATAQSVGKAKEVGVRKILGARRLQ
                     LAFRFVSESFILTAISFMISLLLVFLLLPGINTFTNKTLTLNLYSINIIILLLTIFLL
                     VVILAGSYPSFILSSFKPIETLKSKISPKGSGKRVRTFLVIFQFALSAFLVVGTMVVY
                     KQLNFMRKQELGFTKERIIVLPTRFQASNSQGFKLLKDELLNDSEVVSTCLSDRIPGK
                     QLNNNVVRLGWSDDAEWSDMRYLTTDEEFRELYNLKLVAGRWFSQEFPGDREKSFLLN
                     ESGVRQLGFSSPEDALGKKLDWQDRQGEVIGVVKDFHFVSLNQEMFPFIIVMNRSNPG
                     YLSIKLRTDNYSSIIERIRTKYEELIPQGVFEYYFLDEDFDIQYKADERFMYLFTIFS
                     AIAIIIACLGLYGLSAFTAESKTKEIGIRKALGATVFQIFILLTGRFSRLILISLVVA
                     LPFGAWAMNNWLKNYPFRTEFSPWLFILAVVGLLIIAVITVGFHSIKAARLNPANTFR
                     EE"
     gene            complement(13488..>13800)
                     /locus_tag="VGA21_10885"
     CDS             complement(13488..>13800)
                     /locus_tag="VGA21_10885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651058.1"
                     /translation="DLIFRNSKFSEVSAILDDADMLLETSLTDGGDYTFKVDDGDLDM
                     RILDGGGEFDITFDDGRIIATDEFKIVERDEHAQLLRLSDGNARIRMHANDGRVSLSR
                     L"
BASE COUNT         4294 a         2523 c         2846 g         4137 t
ORIGIN      
        1 aggaaaaggt aagaattcgg aaatttactt tgactccagg atccggattt tttctttaag
       61 tgtactaagc gctaaatcta tttcacgaag atgggttctg aaagagagta ccgccagtcg
      121 tagaaatact tcaccattaa tggtagtaga agaaatgaag acccggccat ccttatgcat
      181 ttctttaaca agagatcggt tgaattcatt aatatctccg gagctgggtc tgtatcggta
      241 aatacacact gagagctccg gttcgggtcc cacttcaaac ccattaatct tctgaagttc
      301 ctgataaaaa taattagtaa gaagaatttt ttcttccagg caatccctaa aggctttcag
      361 accatggatt ttcaaaggta gccatagtcg cggaccccgg aagtgtttgg tgagttcagg
      421 agaaacatcg gccggggaaa catcttctga aacaacatga gcatcctgca tgtaattggc
      481 gaaatactta aaagcctctt tcatcatttg tccgtctttc accagcacaa ctccaagtcc
      541 atatggtaag aacaaacctt tatgtggatc catcaccaga gaatcagatt tttcaatgcc
      601 tctaaacttt gatttaacag aatcacacat caggaagaac cccccgtatg cagcatcaac
      661 atggaaccaa atattatttg tttcgcaaat gtcgccgagc cgggataatg gatcaattgc
      721 gccggtatct gttgttccgg cagaagctac gagaagaaat ggtttcaatc catctgcaat
      781 atccttttca acaaggactt caaatttatc ggcatcaatt tgatgcaggc tattcaatgg
      841 gagtcttcta agaactgatt cttcaagacc cgcgataact atggctttgt caacacaatg
      901 gtgagtttgt ggggaaaggt aaataaccag ctttggaaaa tctgctgcct taacctttga
      961 tgcttttcgg gcggtaacaa tggcgctaag attggcaatg ctgccacctg aggcaagatt
     1021 tcctgcacat cctttcggat atccggctag ttgggccatc cagcggatga gttgattctc
     1081 catgcgcacc gcgtttggat ttgcgaataa aatacctgaa tacctattag taatggctgc
     1141 ccaataatcg ccaatacttg aggtgtacaa accgccgccg ggaatatatc cgagatgccc
     1201 gccagaagcg gggttaattc cggaattatc tacagatctc aaataaggat cgagttcttt
     1261 gaaatcatta attccggatt cctgaaatgg gtcgttcttc caacctgcat cttcgatctc
     1321 ataggttttc aaatctcgaa ttgaattcag gaaatcttcc gtataattca ccatccagtt
     1381 cctgatctct tttctttctt ctacatcagg ctcaaggtgg attgcacttt tttccaattc
     1441 ggtaattttc tctggtagat tcattgttaa taacaaaaag tttaattctg gatattttga
     1501 taaaagttag ctaagttaga aataagttta aaatacatta caatgtctct tactccatct
     1561 aaaatggttc ctctgggtac agaagccccg gactttattc tgccggatac agtttccgga
     1621 caacaaatgt cactcaatca attgaagtct gagtttgcca cagtaataat gttcatctgt
     1681 aatcactgtc cctatgtgaa gcatgttcgg gaggaattag tccggatcac caatgattac
     1741 actaaacgtg cagttagttt tattggaatc agttcgaatg atattaagga gtatccggaa
     1801 gattcacctg gaaaaatgaa ggaagaagcg atcaaatatg gctactcatt tccttattta
     1861 tttgatgaaa cccaggaagt agccagggac tatggcgctg cctgtactcc tgacttttat
     1921 ctttttgaca agcgtatgaa gctggtttac cgcggtcaac tcgatggcag cagacccggt
     1981 aatgaaactc ctttaacagg aacagatttg cgcaacgcta ttgatgcggt tttgagcggg
     2041 aatccggttt cggatctcca aattcccagc gttgggtgca atatcaaatg gaaggattaa
     2101 ttcacgccaa aaaaaagaag ccacctttcg gtgactcctt caacactcaa aacacctagt
     2161 gcaatcaatc aatggtttga aatccagact gataatttct ttcgcttttt aagcaccaat
     2221 gccactatcg cggctatgac tccaatggtt atcaaagttg ctgttttaga tttttgattc
     2281 atggtaagcg caaataaaca ccgatttatg gaagcttact gataaaatca tcccaatgat
     2341 ttgtaaaaaa gtatcaatcc aactttaaac ggtatgaaat tgacctaaaa ccccggattt
     2401 tcaataaaaa cccaaaaagt ttaaatacta aaaattcttg cctggtatgg ttcgagagat
     2461 ttttgtgcaa tcaaacctaa aaatgaattt caaacttcga tgaactattt ttatatggca
     2521 tgccaaagcg aattttgtca ccaaagcaaa ctctaactac tcattaataa tccctttaca
     2581 tcaaaggctt gtaacaaatg ccgttcttca tgacgggcaa taatcgctat ggcgtgagaa
     2641 accttatatg taacaaagaa agaggccgga gagtgaatca caatgtcttc aaagcctgac
     2701 tccctgattt ggtttgacct tgaaatgagt tcattattgt gtttaataaa acgcaaggtt
     2761 atatcggggg taatttcgga ttgagaaggt ctgaaaactc cgggagatct gtaaactttt
     2821 cttcgctccg gtcctaaagt ttttatcata aagtttccaa aataattgga gaaaggactc
     2881 agacgactcc ataggtctga tttgtacttc ctgttaatta ttgattcaaa gacctgaaga
     2941 taggttgaat tagtggtaat aatgtggtca aggcattggc caattgacca tttatcagga
     3001 tgtggtttcc aatttaattg gtcaattgtc aaagaggaaa actcctgcct ggcttgctca
     3061 gagatttcat taattttagt cagatactcc attgaaatca ctcctttaat ccggccataa
     3121 aattgtttac gatcctttca aagtcgttct tcttctcaat aaatggaaaa tgaccgcaat
     3181 ctcttaatac ccaattctgt ccattcggca ttgttttgta aagaatttca tcagagactt
     3241 tataagtggt ttcataatct ccataaataa tcaaagtcgg cacggttacc tgagccaatt
     3301 catcggtaag gtcaaaatca atcagatcct tttgcaaacc actcaacagt ccgcttgtcg
     3361 ttataaatct ttccgtaatc gacaggttta aactatcagc aaaatttttg ttaaagaatt
     3421 gtcttttgaa gctcagacgc attagttttt caatagcggc agcttcccca ttcgccatct
     3481 cttcggaggt tagaatctcg ttaaattcaa tactgtcttc aggtgaaaca tttgcagcat
     3541 ccagcaggct tgcgctctgc atcatttctg atgttggagc aaccgatcca attaatatca
     3601 aggaattcag attctccggg tacttaatag cgtagttcat cgcgatcaag cctccgaaag
     3661 aatgagccat gagacttatt ttgcccactt cgaaatgttt tctgattcct tccagatctt
     3721 ctataaaatt ttccatagaa atagaggccg tgtcaacatt gatagaagat tgaccgccgc
     3781 cccgttgatc atagaagatt aattggtact tatctgcgag ggctaaaaaa tgtggaagta
     3841 aatacgtgtg atccaaacca ggacctccat gcagaattac cacgggttca ccgctgccaa
     3901 ttattttgca ataaagttct gtgccgttaa ttgagacgag accgctaaaa ttttctactg
     3961 gattctcatc ctgctccgtg tttttagatg gcttaccgca agcagctaaa agagacatta
     4021 ttaacgcgag caaccagata ttatggacaa atcttctcat ttcgagttat taaatattta
     4081 tcgaaagata taaaacttat gggagattag attgcctcaa atgatcaaaa actgataata
     4141 atcatatttg caaacacaat ttttttatat tattacttca tggtattctc caggccgggt
     4201 agtttgctag tgtctttaat tgaagttgaa gaacaggatt ttgatccttc gagctttact
     4261 tttaaggcga cttacaaatt gaagttctat aacaataacc tgctgctgcg taaaaggatt
     4321 aaataccctt caatatgcat taataaaaat ggaaaaatac tggaatgtcc attatcagat
     4381 ttgaatggtg atgaaattga tgtaataaat attcctgcaa agaaatcaga aacaattagg
     4441 gcaacggtga atgaatcaaa tcctccgttt acggatattg gagagtttac ttacaatctg
     4501 tcctattatc agcacggctc caaaaagcct gtaaaagtgc tcatttccgc aaataattcc
     4561 cggcaataga tttcagattc tccgcttcat agctgaaatt taataaaatt aatactgcag
     4621 tgtaactttt actgatcagt tgcggtttcc cttcacaaat aagcaaactg gtcgaccaaa
     4681 cctgaagctg ttggattcac ttacagataa tgcattaatg atgcaggtca agaatggcga
     4741 cctggataag cttggactgc tcttcgaaag atataacagg attttgtttg ctttttttta
     4801 caggctaggg aacgacagtg aggtttgcga ggatttaatt caaaatgtgt ttatgagaat
     4861 tgtaaagtac aaaaagaatt ttaaaggtga aggccagttt aagcattgga tatttcacat
     4921 tgctcgaaat attcacgctg acaattacag ggtaaataat aaattgggta aacgagaatc
     4981 tcttgaaaat tatagtgacc gtttaacaga tcatggagaa ttagtgacaa aagatgaagt
     5041 ggatgataat gtttcgttgt taaagaaatg tatttctatg atggatgaag aaaaaagaga
     5101 attaattacg cttagcaaat tggaaggaat gaagtataaa gatattggga caattattaa
     5161 ttgttctgaa ggaactgtga aagtgaagat tttcagagcc cttaaagagt tgaaagatat
     5221 atttcaaaaa acaaaattga accatggata aggaaagaca aaagcagcta attgcaaaat
     5281 tcaataacct tgaaatttta cctgaagaat taattctttt agagaaggca attgaaattg
     5341 gtgaaattgg tcttcaggag ctaaaggaaa tttcggacct ggatcgccaa cttgatttcc
     5401 ggattcctga gcccggtgaa aatttgcgag cagcgtttta tgcaaacctc gctaaactaa
     5461 aagcttccaa atccagggga aacgcgatta ccggtcttaa catctggtta actaatctcg
     5521 gttttagtca atctgcaata cggctggctt atgcatttgt attagttatt gttgggttcg
     5581 gaattggtca gtggacgcaa tcttctattc cttatgaaaa gattgacgct ctgactaccc
     5641 aggtttccga aatgaaggga cttatgatgc ttaccttaat caaagagcca attgcaaccg
     5701 acagacttaa agctgtaaat cttgctatgg atgctgataa ggttgatgaa aggatgatcc
     5761 ttgctttgct aaacacatta aataatgatg ataatgtaaa tgtgaggctc gcatccgtgg
     5821 aagtattatt acgatactcc aataatccag ttgtgcgaca gggacttgtg gaggcgatac
     5881 ccaaccagga taatgccacg atgcagtatg ctttggcgga agcaatggta ctgatgcagg
     5941 aaaaaaattc cattgaaaga cttcaggaac ttatgcaaaa agaagacctg gatgaagatg
     6001 ttagaaataa aattcatgaa agtattcaaa aactaagtta atatgaaaac actaattatt
     6061 atctcaacat tttgttttgc atcgattgcg gctaatggtc aggaaaggaa gcaaactatc
     6121 aataaggaat atgatttggc tggtccggca aaggtgttaa ggatttctaa tgtcaatggg
     6181 tttattaaag tggagggata taccggatca aaggtgacaa tggaaatcaa tgagagtctg
     6241 aaagcaaaaa cccaatcttc ccttgaagac atgataacca aacttagcat tggaacagag
     6301 gaatacggag acaccctgga gatatttgtg gatggtatgt gtaagtgtga ttgtgaacgg
     6361 ggacggagat ataactggaa caactgtgat tttaagggta gttttcttta tgactttgcc
     6421 gtaagagtgc cacaaaattt gaatgtcatt ctttccacgg ttaataaagg agacattgaa
     6481 gtaaaaaatg tatccggaaa aatacaggcg aacaatgtaa atggagcaat atacctgaat
     6541 gatgtaaggg aggctttgaa agtgagaaca ataaacggag atgtggagat tcactacact
     6601 gatctgccga aagaaaattc tgaatactat acccttaatg gggatttaac cgtcacttac
     6661 ccctctgcat tatcagcaga catgagtttc aaaagtttta atggtgagtt ctatacgaat
     6721 ttctccatca aggaacggtt accggcaata gccaccacaa ccgagcgggg aggtcatgga
     6781 acaacctata agatagatga aacaacctct gtcagagttg gaaacggagg agtgaaactt
     6841 gattttgaaa cctttaatgg tgacatcttt ataaagaaaa atcaataaag acaaagaaat
     6901 aaagggatga ataaatttaa attgagaaaa atgaaaaagc aaatgattaa actggttgga
     6961 gtatttatac ttattatcgt atttcaggcg aaagcgcagg aatatgatca aatagtaata
     7021 cctctttcca gtccggacaa aaccggtgca ttgaaacttt ccttgccaaa tggaacaatt
     7081 aaagttacgg gatattcagg caaagatgta attttgaaag tcagcaccga acagcaaaaa
     7141 attaataaag gaacaaagga tggcttgatg aaaattccaa acagatccct tgggataaca
     7201 gctaaggaat ctgaaaatct tgttaccgtt agtggcggtt ataaccggga tgtaatcatg
     7261 gaaattcagg tgcccactaa gtttgattta aaattaaccg gtcataatgg tggagatttc
     7321 accgttgaaa atataaacgg caatgtcgaa atcacgaacc acaatgcagg tattgctgcc
     7381 agaaacattt caggagctgc aatccttaat tctcacaatg gcgacatcac cgccactttt
     7441 gtaagtgtaa ccccagacac gcccatggcg tttacaacat ttaatggaga tgtggatatt
     7501 acattccctc ccacagtgaa agctactttt aaaatgagat ccgaaagaaa tgacatcctt
     7561 acagattttg aactgaattt gaaggaagcc aaaactaaag agtctggcag ggatgaggat
     7621 ggtgtttata aaatctccat tgaagagtgg acggttggag atgtaaatgg aggcggacct
     7681 gaaatgttgt ttaagacgta taacggtgat gtcataattc gaaaaaagaa ataatcatga
     7741 gaaagttaac ttcagcgctt gtcattttgt tgcttggagc aactgctttt gcgcaatctt
     7801 ccaggtatga ggatttggaa tacttcgata aggtaattct gaaaggaaat gtgaaaaaag
     7861 tgtctcttga aaatggagat cgtgaaattg ttgtagatgg agctgacaaa aatagcgtta
     7921 agctttccag gttggcagga attatcacgc tggagataac ttcttcaggt ccggtagcag
     7981 tgtccataac taacggaagc ctgaaatgga tcgaagcttc ttcagaaact gaaattgaag
     8041 gagccgaata tctgagtggg ggcaaaggca aatatctggt cgtaggaatg aaagattcta
     8101 agggtcggtc gaataatgaa aatttcaatt tcaattttga cgacttcgat tttgatttcg
     8161 actatgattt tgattccgac ttcgacttcg attttgattt cgactatgat tttgatttta
     8221 acttcgatag agactattcg tataactacg actacaaata cgactacaag tacgaccgga
     8281 aggttaacag agtaaaagag aaaaacgata actaattcgt ctcagggaga aatataactt
     8341 ttcccttatt ttttccgttt atgttttata tataagaaga taatattgaa aggtatatat
     8401 cttggtgaat ttgaggatgt tgaacctctt tcagttgctt actaagggaa gaaacatatg
     8461 cagtatcaat tgcggaaaga gtttaataga aagcgacttg tattttgaag agcagtcatt
     8521 agacataaac cataacggtc atgcttaaaa actatataac cattgcactc cgaaatatta
     8581 cccgtcaaaa agtttacact tttattaatg tcagcgggct ggccattgga attgccgctt
     8641 gcatattaat tttattattt gttaaggatg aattaagttt tgatcaatac catgaaaaat
     8701 ccgaacggat ttaccgggtt tcccgggaat ggatgaacaa ggatggcgag accagcctgc
     8761 atctgggtca tgtagctccg ccttttggac cattattgga aaatgatttc agcggaatga
     8821 ttgaatatgc ggttaggttt cagtcaggat atagtcccct tgtgaaggca aatggaaaag
     8881 acattcagga agaaagattt ttctttggtg atcagaatat ttttaaggtt ttctcctgga
     8941 aagtgctgga tggagatccg gaaacagccc ttttggaacc caatgctgtg gttttgacta
     9001 aaaccacagc tgaaaaatat ttcggatctg aaaatgtggt aggaaagcaa atcacctata
     9061 ataattttgg gctaagcatt gaaatgaaag tcaccggtgt aatggaggac gttccactca
     9121 actctcattt taaatttgat tttctgggat catttaaaac aattgaaaat gtgtttggcg
     9181 cggataacct tgccaggagt tggggaagta acaattatgc aacttacctt cttcttggtg
     9241 aaggcactgc ccctgaacaa cttacagctg aatttcccgg tttccttaat aaccacatgg
     9301 gaccactcga cgacggtaga aacaggtctg acgtaaatcg tctgcattta tggccgcttg
     9361 catctattca cctttactca caccttgatt cggaagtgga acccaatggt gacattgctt
     9421 atgtgtatat atactctatc gtcgcgctgt tcattctcgt aattgcctgt ataaatttta
     9481 tgaatctatc cacagcaaga tcggctaagc gtgcacagga agttggcctt cgaaaagttt
     9541 tgggtgcagt aaaaggatta ttgatgcgcc agttcattct tgaatcagta attttttcaa
     9601 taatatccct tctgtttgcg gtactgtttg tactaatgat tcttcccttt tttaatgatt
     9661 tcgtacaaaa ggatttgagt ctcaatgtta tggaaaatcc ctttgtggcg cttctccttg
     9721 ttggtataac cctattggtt ggcctggttg caggtagcta cccggcattt taccttgcct
     9781 cttttcaacc tgctactata ttgaaaggct ctcataaagc atcagggaaa aaaatcagtc
     9841 tccgatctgt gctggtgatt tttcaattct ggatctctat tgttctgata atcggagttg
     9901 gcattgttca ggaccagctg gaatatatgc gcaaaaaaga tttaggcttt aacagtgaca
     9961 acatgcttgt gttgccaccc agcaatgaga tatataataa ttttgaatca gtaaaacagc
    10021 gactcgaaaa taacccggca attgaacagg taacaatttc atcccgcgtg ccttccggac
    10081 gtttgttaga ctctcagggt ggggaggcag aaataaacgg tgaaatggtt gacattaatg
    10141 ttagggttgc agatgtacat gtagaccatg agtatttgga gcaattggag attccgctgg
    10201 cagctggccg gtatttcgac agatcaagag catcagattc atcattggca tttattatta
    10261 atgaagcaac tgtaagagca ttaggatggg atagtagtga agaagccatc ggaaaaaaat
    10321 tcaattatgg ttcaaggcag ggcggtgaaa ttattggtgt ggtaaaagat tttcattttg
    10381 aatcattgca ccaaccaatt gttcccattg tctttctggt aacgcaaggc cgattttcaa
    10441 atgttattgt taaagtgaag aaaggtatgg aacaggaagc ccttgcgttt cttcaggacc
    10501 aatggcaatt tctaagacct gggtttcctt ttacctcgtt ttatatcgag gaacaattca
    10561 gtaatcaata tggtaacgaa gacaggcttc ataagattgt gaaatatttt tccggcctgg
    10621 cggttctgat tgcagctctg ggacttttcg gccttgccag ttatacggcc gaacagcgaa
    10681 tcaaagaaat agggataaga aaagtaatgg gtgcatccgt gccccagatt ttgattttac
    10741 ttaccagtag ttttacccgg ttggtactaa tcgcttttat tctcgctatt cctcttgcct
    10801 ggtttggaat ggatgcatgg ctatctaact ttgcattcag aggacagctt caggtgatgt
    10861 cttttgcaat aggcggagtg ttggcccttt taattgcctg gattacagta atttcacaaa
    10921 ctttgaaagc agcgatgaca aatcctgttg attcaatcag gtatgagtaa gaagctgaat
    10981 tgagttctga atggaccagg gaaatccttt tccttctctt taaattattt gtttgaaact
    11041 gtaactttta tcattagggg aggtatacct gcttaaattc aaatatggaa ttccttcgcg
    11101 aaaacctgat aacttcagtt cgttcaatac tcagaaataa attctttgct cttctcaata
    11161 ttctgggttt aacaatcgga atcacttcat tcatcattat ttcgctctat gtatgggatg
    11221 aaattaccta cgagcaatgg catgccaata aagacactat ttacaggctt gaggtggagt
    11281 attttcttcc caatgacgga ggttctcagt acatggcggt tactgccccg attatttcgg
    11341 aaattgtgca gaaagattat ccggaagtag aatacagagt gagaattcaa gagtggcgtg
    11401 atgagctaat cgagaattat gcgggagaaa aatactatga atctttgtac tttgccgact
    11461 cggctttttt ccagatgttt tcttttgaat taaatgcccg ggtaaatgaa atgcctttaa
    11521 gtgaaccgta ttctgcggta attaccgaag atttagcgcg aaaatacttc ggtagaactg
    11581 atgtacttgg tgaattgctt agactcaaat cctttgataa agatgtcagg ataaccggaa
    11641 ttctaaaatc ttatgaatct aacactcatt taagaccgga gtttctggtt tcaatggcca
    11701 ccttgaaaag tgaaaacgaa ttgccacaac actggtggac tttcaacacc tattcttatt
    11761 taaaactcat tacaaattca gacgcgaatg ccctggcatc aaagattaag aacatttcag
    11821 gaagatatat aagagagcag gaagtttcgt caggctatca tcaggaatat tcactgaaaa
    11881 acataaaaga catccacctt aattcacata tacggggtga aatttcttct aacaataagg
    11941 cttcatttgt atttcttttt ggagctattg ggattttcat tatcgtaata gcttgcataa
    12001 atttcatgaa ccttgctacg gcgcaatctg ttgggaaagc taaagaagtt ggcgtgagaa
    12061 aaattctggg cgcgcgccgg ctacaacttg cttttcgttt tgtaagtgaa tcttttattc
    12121 taaccgctat ttcctttatg atttcccttt tgctggtttt ccttttactc cctggaataa
    12181 acacttttac aaacaaaacc ctgactctca atttatactc aataaatatt attattttac
    12241 tcttaacaat ttttctgctg gttgtgatcc tggctggaag ttatccatca tttattttaa
    12301 gcagctttaa gccaattgag acccttaaat caaagatatc tccaaaagga tccggtaaga
    12361 gggttcgaac ttttttggta atattccaat ttgctttgtc tgctttcctt gtcgtgggta
    12421 caatggtggt ttacaagcaa ttgaatttca tgagaaaaca ggaattgggg ttcacaaagg
    12481 aaagaataat tgttttgcca actcgtttcc aggcgagcaa cagccagggg ttcaaattac
    12541 taaaggatga attgttgaat gacagcgaag tggtaagcac ctgcctctca gatagaattc
    12601 caggtaagca gttaaacaat aatgttgttc gtttggggtg gagcgatgat gcggaatgga
    12661 gtgatatgag atacttgact accgatgaag aattcagaga actgtataac ctgaaattgg
    12721 ttgccggcag atggttctca caggaatttc ctggcgacag ggaaaagtca tttctgctga
    12781 atgaatcagg agtgcgccaa ttgggattct catcaccgga agatgccctt ggtaaaaaac
    12841 tggattggca agaccgacag ggtgaagtaa ttggtgtagt aaaagacttt cattttgtgt
    12901 cgctgaatca ggagatgttt ccatttatta ttgtcatgaa cagatccaat ccaggttatc
    12961 tttcaataaa actccgaact gataattata gctcaattat tgaaaggata aggacaaaat
    13021 atgaagaact gatccctcag ggagtttttg aatattattt tttggatgaa gacttcgata
    13081 tacaatacaa agcagacgag cgattcatgt atctctttac cattttttcc gcgattgcga
    13141 taataattgc ctgcctgggt ctgtatggat tatctgcatt tactgctgaa tccaaaacca
    13201 aagaaatagg cataaggaaa gctctgggtg caactgtatt tcagattttc attttgctta
    13261 caggaagatt ctccaggctt attctgattt cccttgtcgt agctttaccc tttggagcct
    13321 gggcaatgaa caactggcta aaaaactatc cgttccgaac agaattttcg ccctggcttt
    13381 ttatcctggc tgtggttggg ttactgatta ttgcagtaat tactgttggc tttcattcca
    13441 ttaaagccgc cagattaaat cccgccaata ctttcagaga agagtgatta taaccggctt
    13501 aggcttacgc ggccgtcatt agcatgcatt ctgattcgcg cgtttccatc agaaagtctc
    13561 agcaattgtg cgtgctcatc tctctcaaca attttaaatt catcggtagc gataattcta
    13621 ccgtcatcga aagtaatatc aaattcgcca ccgccatcga gtattcgcat atcaaggtcg
    13681 ccgtcatcaa ctttaaaggt ataatcacct ccatcggtca atgaagtttc caatagcata
    13741 tcggcgtcat caagaatggc gcttacttca ctgaatttgc tatttctaaa aatcaagtct
//
LOCUS       DASWHX010000162        13628 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_164166, whole genome shotgun sequence.
ACCESSION   DASWHX010000162 DASWHX010000000
VERSION     DASWHX010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 13628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13628
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164166"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1299)
                     /locus_tag="VGA21_10890"
     CDS             complement(<1..1299)
                     /locus_tag="VGA21_10890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651059.1"
                     /translation="MIKGRFLLHFFAFTLGLLFLGVTAVFSQVTITQGTAPTICGTGT
                     VTLSDIVIDEVNDANNEAIGGANGSGSGVTFILELNNANFSFVNGAGTATSTSGTDDV
                     SSISLGGWTATTVTITFDFNNTGGGVNDKIDAITISGLQVNSGIAGNESYIITRQGGS
                     VSINGLADGTQVGSGLKASVVVANAGSDVSICSGESVQIGGTPTASGGGDSPIYTYSW
                     SPSTGLSSTTSANPNASPTSTETYTVTVTDTSTGCFDTDDITVTVTGSVPTITIAPAT
                     PNPACKGDFIDLSGSSVVGATGGVWSTSGDGGFSPNTTTVAANYQPGANDIINGSVTL
                     TLTATGGCPGSENTVISFEATPATPTITVTLGTTTFCEDGGTTSVTLQSSAAPNGGTY
                     LWYKNGISTGITTQDITLGTVAESGSYTVEVTDGAGALCTS"
     gene            complement(1426..2679)
                     /locus_tag="VGA21_10895"
     CDS             complement(1426..2679)
                     /locus_tag="VGA21_10895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651060.1"
                     /translation="MKHLVGKIINLLIIISIFTTIGCDTSSGLEPGFEKVFYKLYGIQ
                     GDQVGKDLKQLPDGSGYIIIGNSDTTGFSNGTIDQDIYLVRTDAQGNEMYHRTFGGNR
                     KDESVAIEILPSGEFLFVVNSENTNQKIDAVVYKVPDDEFAVYDSAVFGNPLEDNIIN
                     GLSLISNGIIICGTTTDLDRITKPNPPSDLRDIYTVKMDFNFVEDPVWTNVYGYSGDD
                     EGIKVVELSSGSFAFFGTTDTPTPANSQTDLHTFFTFPTSVNGLPNGILSQYGTAGEE
                     FAKSVTVTSVNSFAMIGTSMAATSSIYVTFLRSDLSAPSGNGKSINVSNTEGISIYEA
                     TGGGFIITGNQIVSPENKDIYLAKIDNEGIVIWSRTFGTDGLDEAGSLIQSQDGGIVL
                     TGSFQLENQFKMVLIKAGPDGEMNF"
     gene            complement(2683..3777)
                     /locus_tag="VGA21_10900"
     CDS             complement(2683..3777)
                     /locus_tag="VGA21_10900"
                     /inference="COORDINATES: protein motif:HMM:NF024957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HEX9651061.1"
                     /translation="MLLAVAESWAQTACSQKLAEAQESFNQGHFYGIPSMLKNCIDAG
                     FSNEQRIEAYKLLTVVYLYIDDPIGAENSYLELLELDPEHKVDPAIDPIEIIYLNDKF
                     TTTPFFTLQLLKLGINATIPFVINNYSVGNTNLNTENYEIGIGFTLGAGVDYNLSDYF
                     SIGLELALERRSYNWNNTIFDQDGQNYSENILGLSIPLFLRITYPTKKWHPYVYGGYA
                     LKINLLSGFSDMRFDNNTFTDDGGNATQPVIVPDIQNANELRNTLNTAIVAGLGVKYR
                     LNYQYLSFDIRMNAGLLNHQNVGERYLLTNIEANSLEEESSLATTLGIVLDDIRINDL
                     TFTVGYVKPLYKPRKKVRGAKGIRGIIKRN"
     gene            complement(3816..6944)
                     /locus_tag="VGA21_10905"
     CDS             complement(3816..6944)
                     /locus_tag="VGA21_10905"
                     /inference="COORDINATES: protein motif:HMM:NF044287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651062.1"
                     /translation="MLNYEIHMEPREAEVLEAEIREDLKLSNPFPGLRPFSIDECHLF
                     FGRESQVDEILQKLAENKFAAILGYSGSGKSSLLNCGLVPVLFGGFVTEKSPNWNVII
                     SRPGIAPIDNLAEALLSKDPEYQSASEEEKIINKTIVSSVLRGSNTGLIDIARRHNLA
                     TGENLLIMIDQFEELFRYREEERIAESEDESTEFVNLLLKAVEQDEVPIYLTISMRSD
                     YMGACSKYPRLTDKINQSNYLVPQMTREKRRIAIEGPIAVGGGRISERLVSRLMQDMG
                     DNQDQLPILQHALMRTWEYWVEHKEPGEPMDIRHYNAVGRMSEALSQHANEAYDELST
                     RHKEIAEILFKTITEKGVDNFGIRKPTKVGVIAEIAGVDAFDVIEVIEHFRKPGRSLL
                     MPAAPFKLTPDSVIEVSHESLMRIWTRLKNWVEEESESAQMYLRLSDAAAMYQVGRTG
                     LWRPPDLQLALNWQKKQKPTRIWARRYDEAFERAIVFLDTSRITYEAEQRNQELQQKR
                     LLRRTRAVAVILAIAAIVAIFFFIFGITQRIEADTQRVLAEQRAEEAETSKIAAEQSA
                     DEAIKERENALEQTRLAESNAQIADFAKRDALANLEFAEQQAEIARKQTAIAQAQTKL
                     ADSARLVAEEKTAEALRANEEASRLLLLSIAQSMSVKALNVKDPDLKGLLAQQAFFFN
                     NDNGGKEYDNYIYEGLNNAMSAYYGVTYNKFSGHQNAVRTVAFSQDGKNIFTAGSDGQ
                     ILKWAFDSRDSIPMLLGQKAYPRKMIKVSPDNKWLAVASDSSSIELYDLVMGGRPIEV
                     TGHKGLVYDIEFTPDSKGLYSSGYDQTLRYFDFAKSRLIKNLPGKMLSITVSPDGSRI
                     VTGGLDGNVYIYDTQAFNEKLLLELPGNIIQSVKFSKKGDLIGVGDQFAIVRLLDSQS
                     GEVLNELIGATAQISDVEFSKDDKLVAAGSRDGTVHIWIMSNLDDLPLNLTGPEMSHI
                     WDVDFSPDGEYIVASSSNGVIRYWPTHAETLSQEMCDLIIRNFTQREWERYVGNKVEY
                     QITCNKSLSLRK"
     gene            complement(7052..10225)
                     /locus_tag="VGA21_10910"
     CDS             complement(7052..10225)
                     /locus_tag="VGA21_10910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651063.1"
                     /translation="MLNLLYRALNVLPEERGRVILLLGNGFFMGIFIATYKIASDTLF
                     VGEMEEYISEGIFVSGFLGVCSTALFVYLQNRISFSRLAILNWIIILSISAALYYALI
                     NYSDQYRGILVFLLYVLQGPLAAITLLGFWGIFGRLFDLRQSKRIIGGIDTGQLTAQI
                     LTFFTIGSGIAALSRTEDLLVIGFYSVIGSLIFLILITRKFDLNLVNRPDQRKTKETK
                     FMELVKNNYVILLSLFLAFSVISLYLVENSFLNVLSTQYAESSGTELQEFIAWFYGSI
                     YILSFVFQTFFNDKIISNYGLKVSLFILPVVLTFFTVTSILAGSFFGYNPDAANTTNF
                     FWFFLFIALSKLFTSFLRDSLENPTYKLYFMPLDNRIRFDIQAKVEGVVNEFAKFIAG
                     GFIILLGLLSFFELIHYSYFLIVVIGGWMYIIVKLYEGYRNRVKIKLESHEIDPEKLL
                     KPRNQVVNELEKELYHADPEKAVFSFRLLEKIDPNSIAPSVNSLMKHEQEQIKDYAQT
                     KLNELRGLSVSDEYVIAFDPTRFINLPKRVVSGLDLENLFTTGEISYQRISKLSRSEN
                     PQDRLYAAELISNMEDTELKYLLVELLGDPDDKVRITAMKAGVKNYNYEVLNAIIENL
                     DYLRLSNVAANSLVQIGNNALNALDSAFYRVGQSVTVMTKIIRILGRIGSRPAMENLW
                     AKIDFPDKIVVSEVLDSLGDCNFKASIRQITRIKYAIENDIEDIAWNLAAIGEIPDNQ
                     IGRDLRLAVEEENHHDIEHIYVLLSMLYDSKTIQLVKENLSSRTSEGITYAIELLDVF
                     LSDDLKQKIIPVLDDLPVFDKARKLEAFFPRESLGSKEVLKFLINRDYNQTNRWSKAC
                     ALKQIAMMKIHDYSIDLIANLFNPDLLVSETAAWALFHLDPKLFHENISRLNPEFKRL
                     ISETVIGTKENDKKILKLEKVKFLKNMRIFNEVPGRILANLVDYIEEVTMHEGTSYPL
                     QGNNSDYFFIIYSGEVNYYSDGKKVRNMRRQYFIGELADSEDADSSLLIAQEKALLFK
                     ISKEKFYEQLSDNFNFANQVIRYLYAG"
     gene            10418..11353
                     /locus_tag="VGA21_10915"
     CDS             10418..11353
                     /locus_tag="VGA21_10915"
                     /inference="COORDINATES: protein motif:HMM:NF024806.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9651064.1"
                     /translation="MIIVRSAKLILLWFLIFGSFSGVFSQNYYDQNFRKGYYYLDVSI
                     SQAAEYFGKAIMNDSTQPEAYFYRGLAYYKMDEFGNAIRDFKKSLEIDTTLVKINVFI
                     SFAYRGLNDWDKALESFNEYMIRTSIDSTSFNWLMRGKLKQLNGDITGALEDFKKAVF
                     LSPEQEKFRFYLFLTELEQKNFQAALEQINEMINLNPEFYGYYFYKGNIYFQMMKFNE
                     AIENYTVSIEKNNNNPDGYFRRAWSADTLSNYTTALADYGRAIDLKSDDAAYYAKRGN
                     LLYRMAKIDDACKDWAEANKLHYYKDYEKLNKLCK"
     gene            11379..12203
                     /locus_tag="VGA21_10920"
     CDS             11379..12203
                     /locus_tag="VGA21_10920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009579014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HEX9651065.1"
                     /translation="MDNRKYLPILIVGGIALIVILLLSNSIFLTIEPGKAGVLFKKFG
                     GGLDKERIYYQGFQVIAPWNTMFIYDVREQTAEESMDVLDKSGLSISVDVTVRFNPQP
                     VKIGFIHERFGIDYKNTLVIPEVRSTVRQVMGRFTAEEIYSTKRAEVEGAIKTETENV
                     LGSVNNNIVMNAMLIRSINLPEQIKMAIENKLTQEQEALAYRFRLDKEESEAKRKQIA
                     AEGEARANQIINSSLTPSLLKMRGIEATTSLASSPNSKIIVIGSGSDGLPLILGSN"
     gene            12303..13589
                     /locus_tag="VGA21_10925"
     CDS             12303..13589
                     /locus_tag="VGA21_10925"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009054732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HEX9651066.1"
                     /translation="MSDQVELKIKDKNYQFPTTKGSEDEMAIDISSLRSQTSYITLDP
                     GFKNTGSTTSAITFLDGEKGILRYRGYPIEQLAEKSNFLEVSYLLIYGELPGKAEFEE
                     FRKQITRRSLVHEDIKNILMGFPSAAHPMGVLSSLVSSLTAFYPQSLDPHRGKDKINL
                     SIIRLLAKFPTIAAWTYKNKIGQTFNYPDNSKDYCENFIEMMFKVPTEPYKLDPVVAS
                     ALDKLLILHADHEQNCSTSTVRLVGSSQASLYSSISAGICALWGPLHGGANQAVIEML
                     ENIKEDGGDYKKWMQKAKDKDDPFRLMGFGHRVYKNFDPRAKIIKGAADGVLDSLGIK
                     DPVLEIAKGLEEVALSDSYFVERKLYPNVDFYSGIIYRALGIPVEFFTVMFALGRLPG
                     WIAQWKEMIENTEPIGRPRQIYIGATERDYIDYTKR"
BASE COUNT         4082 a         2831 c         2589 g         4126 t
ORIGIN      
        1 actggtgcac agcgctccgg ctccgtctgt aacttctact gtatagctgc cgctctccgc
       61 taccgtgccc agggtaatat cctgagtcgt gatgccggtg gagatgccgt tcttatacca
      121 taggtatgtc cccccgttgg gtgccgcgct gctctgaagt gtcacgctcg tggtgccccc
      181 gtcttcacag aaggttgttg ttcccagggt taccgtgatg gtcggggtcg ccggggtggc
      241 ctcgaaagaa atgacggtgt tttcagatcc aggacaacct cccgtggctg tcaaagtcag
      301 tgtgaccgag ccatttataa tgtcattagc accgggctgg tagttcgcgg ccacagttgt
      361 tgtgtttgga ctaaatccgc catcacccga ggtagaccaa accccgcctg ttgctccaac
      421 gacagaacta ccagaaagat ctatgaaatc ccccttacaa gcgggatttg gagtagctgg
      481 ggcaatcgta attgtaggaa cagagcccgt gacagtgaca gtaatatcat ccgtatcaaa
      541 acagcctgta cttgtatcag ttactgtgac ggtatatgtt tcagttgatg ttggacttgc
      601 attcggattt gctgaagttg tagaactcaa accggttgaa ggagaccaac tgtaagtata
      661 tattggactg tctcctcctc cagaagcggt gggtgtaccc cctatctgaa cagattcacc
      721 ggagcaaatt gatacatcag atcccgcatt agcaacaaca acacttgcct ttaaaccaga
      781 tccaacctga gttccatccg ctaaaccatt aatactaacg gatccacctt gtctggtaat
      841 gatgtaggat tcattccctg caattccaga attaacttgt aatccagaaa tggttatagc
      901 atcgatctta tcatttacac ccccaccggt gttattgaag tcaaaagtaa tagtaaccgt
      961 tgtggctgtc cagccaccaa gcgaaattga ggatacgtca tctgtacctg acgttgatgt
     1021 agctgttccc gctccattaa caaaagagaa attcgcgtta tttaattcaa gtataaatgt
     1081 aacgccgctc ccggagccat ttgcccctcc aatggcctca ttatttgcgt cattgacctc
     1141 gtcaattaca atatcactta gtgtgactgt tccggtgcca caaattgtgg gcgcagttcc
     1201 ctgcgtaata gtgacctgtg aaaaaactgc agtaacaccc aggaacaaga gtcccagggt
     1261 aaaagcaaaa aagtggagca aaaagcgacc tttaatcatt taccagtgtg aaagccagag
     1321 ggtttagctg ctatttattc attcaatctc acaaaagtaa cccacatcag ttacacatgt
     1381 gatacgaatt ttacctaaaa taaaaaattc aattaattac ctaaatcaaa aattcatttc
     1441 tccatcagga ccagccttta tcagcaccat tttgaattgg ttttccaatt gaaaacttcc
     1501 ggttaaaaca atcccaccat cctgtgattg aataagtgat ccggcttcat ccaatccatc
     1561 agttccgaag gttctgctcc atattacaat accctcatta tcaatttttg caaggtaaat
     1621 gtctttgttt tcaggactta caatctggtt cccggtaata atgaatccgc caccggtagc
     1681 ctcatatatg ctgattcctt cggtatttga aacattaatt gatttcccat tcccggaggg
     1741 tgcacttaaa tcacttctca aaaaagtcac ataaatactg cttgtcgcgg ccattgaagt
     1801 accaatcata gcgaaactgt taactgatgt tactgtaaca gatttagcga attcttcccc
     1861 agctgttcca tactggctca ggataccatt tggcaatcca tttacactgg ttggaaatgt
     1921 aaaaaaagta tgtaaatcgg tctgactatt ggcaggagtt ggagtatctg tggttccaaa
     1981 aaaagcaaaa gatcctgaac tcaattcaac tactttaatt ccttcatcat caccggaata
     2041 accataaaca ttcgtccaaa ccggatcctc cacaaaatta aagtccattt taacagtata
     2101 aatatctctt aggtcggaag gcgggttggg tttagtaatc cgatccagat cagtagtagt
     2161 tccacagata ataattccat tggaaatcag tgataaacca ttaatgatat tatcttccag
     2221 cgggttacca aaaacagcgc tatcatatac cgcaaattcg tcatcgggca ccttatatac
     2281 aactgcatct attttttgat ttgtattttc agaattcacc acaaataaaa attcacccga
     2341 aggtaaaatt tcaatagcta cgctttcatc ttttcggttt cctccaaatg tccgatgata
     2401 catttcattc ccttgcgcat cagtccgcac taagtaaata tcctgatcaa ttgttccatt
     2461 ggaaaaaccc gtcgtatccg agtttccaat aattatataa ccgctgccat caggcaattg
     2521 ttttaaatct ttcccaacct gatcaccctg aattccatac agtttataaa aaactttttc
     2581 aaagcccggc tccaatcctg aagaagtatc gcagcctata gtagtaaaaa tactaatgat
     2641 tatgagcaaa tttattattt ttcctacaag gtgtttcaac ttctagttcc ttttaattat
     2701 tcctctaatg ccctttgcac cccgaacttt ttttcttggc ttgtacaatg gcttgacata
     2761 tccaacggta aaggtcaaat cattaattcg aatatcatcc aaaacaattc ccaaagttgt
     2821 tgccaaagaa ctttcctctt caagactatt ggcttcgata ttggttaata gatatctttc
     2881 cccgacattc tgatgattca gcaacccagc gttcattctt atatcaaacg acaaatactg
     2941 ataattcaat cggtatttaa caccaagccc ggcaactatt gcagtattta aggtatttct
     3001 taattcatta gcattttgga tatcaggaac aattacaggt tgagttgcat taccaccatc
     3061 atctgtaaaa gtattattat cgaaacgcat atcactaaat cctgaaagaa gatttatttt
     3121 caatgcatag ccaccataaa cataaggatg ccatttcttt gtaggatagg ttatacgcaa
     3181 aaaaagtgga atgcttaaac caagtatatt ttcgctataa ttctgtccat cctggtcaaa
     3241 aattgtattg ttccaattat atgatcttct ttcgagggca agttccaaac ctatggagaa
     3301 ataatcgctt aagttgtagt caactcctgc gcccagagta aaccctatcc caatttcata
     3361 gttttcggta ttcaggtttg tgttgccaac actgtaatta ttgataacaa agggtattgt
     3421 agcattaata cccaatttca ggagttgtaa agtgaagaag ggcgtggtag taaatttatc
     3481 attcaaataa atgatttcta tcggatcaat tgcaggatca accttatgct caggatcaag
     3541 ttccagtaac tcaaggtaac tgttttcagc accaatagga tcatcaatat ataagtagac
     3601 aactgttaaa agtttataag cctcaattct ttgttcatta ctgaatcctg catcaatgca
     3661 attttttaac atggaaggga ttccgtagaa atgtccctgg ttaaaacttt cctgcgcctc
     3721 tgcaagtttc tgagagcatg cagtttgtgc ccatgattca gcaactgcca gaagcattag
     3781 aataagtaaa aggtttaccg tttttcgcaa tctgatcact tcctaagact caggcttttg
     3841 ttacacgtta tttgatattc aaccttatta ccgacgtatc tttcccactc ccgctgagtg
     3901 aaatttcgga ttatcaaatc gcacatctct tgagacaaag tttctgcatg agtgggccaa
     3961 tatctaataa ctccattgga gctggaggca acgatatatt caccatcagg gctaaaatcc
     4021 acatcccaga tatgcgacat ctcaggccca gtaagattga gtggtaaatc atccaggtta
     4081 ctcataatcc agatatgaac ggttccatcc cgacttcccg ctgcgaccag cttatcatct
     4141 ttactaaact caacatcact tatctgagcg gtggcaccaa tcagctcatt taaaacttca
     4201 cctgattgag aatctaataa tcgaacaatg gcaaactgat cacctactcc tataaggtcg
     4261 cctttcttac taaatttaac gctttgaatt atatttccgg gtaattcaag taacagcttt
     4321 tcgttaaaag cctgggtatc ataaatatac acattcccat ctaatcctcc tgttacaatt
     4381 cttgaaccat caggagagac agtaattgaa agcatcttgc cgggtaaatt ttttattaac
     4441 cggcttttcg caaaatcgaa atatctcaga gtttgatcat aaccagatga atacaatcct
     4501 ttgctatcag gagtgaattc aatatcatac accaatccct tgtgacctgt aacttcgatt
     4561 ggtcttcctc ccattaccaa atcataaagt tcaatagaac ttgaatcgga agctacggca
     4621 agccatttgt tatcaggact taccttgatc atttttcttg gatatgcctt ttgacccaac
     4681 agcatcggga ttgaatctct tgaatcaaaa gcccacttga ggatttggcc atcactgcca
     4741 gcggtaaaaa tatttttccc atcctgacta aacgcaactg tgcgaactgc attttggtga
     4801 ccggagaatt tgttataggt aacgccatag taagccgaca ttgcattatt caatccttca
     4861 tagatataat tgtcatactc ttttccacca ttatcattat tgaaaaagaa agcctgctga
     4921 gcgagtaatc ctttgaggtc tggatctttc acattcagcg ctttcacaga catcgattga
     4981 gcaatagaaa gcaggagaag tctggatgct tcctcattcg ctctcaatgc ctcagctgtc
     5041 ttttcttcag caactaatcg ggcactgtcg gccaattttg tttgggcctg ggcaatagca
     5101 gtttgcttcc tggcaatttc agcctgttgt tctgcaaact ccagattagc aagagcatca
     5161 cgttttgcaa aatctgctat ttgggcatta ctttctgcaa gtcttgtttg ttcaagggca
     5221 ttttctcttt ctttaattgc ttcatcagca ctttgctctg cagctatttt acttgtttcc
     5281 gcctcttctg ctctttgttc ggcaagtacg cgctgagtat cagcttctat tcgttgggta
     5341 attccaaaaa tgaaaaagaa gattgcaacg atggctgcaa ttgcaagtat tactgcaaca
     5401 gctcgagttc gtcgtaataa ccgtttttgc tgcagctcct gatttcgctg ttcggcctca
     5461 taggtaattc tacttgtatc aagaaaaacg atggctcttt caaaagcttc gtcatacctt
     5521 cgagcccaga ttcttgttgg tttctgtttt ttctgccagt taagggccag ctgcaagtcg
     5581 gggggtctcc ataaacccgt gcgacctacc tgatacattg ccgctgcatc ggaaagtcgc
     5641 agatacatct gagccgattc tgattcctct tcaacccaat ttttcaatct tgtccagatt
     5701 ctcatcaagc tctcgtgaga aacttctata actgaatccg gagttaattt gaatggtgca
     5761 gcaggcatta acaatgacct gccaggcttc ctgaaatgtt caataacttc aataacatca
     5821 aacgcatcta ctccggcgat ctccgcaatt actccaactt tagttggttt tctgattccg
     5881 aagttatcaa ctcccttctc agtgatagtc ttaaaaagta tttccgcaat ctctttatgt
     5941 cgtgtgctca attcatcata tgcttcattt gcatgctgag acagcgcttc actcatccgg
     6001 cctacggcat tatagtgccg gatgtccata ggttcacctg gttctttatg ttctacccaa
     6061 tattcccatg tccgcatcag tgcatgctgg aggattggta actggtcctg gttgtcgccc
     6121 atgtcctgca ttaggcggct caccaacctt tcagagatcc gtcccccgcc aactgcaatt
     6181 ggtccttcaa ttgctatcct tcgtttttcc cgggtcatct gaggaacgag gtaattgctt
     6241 tggtttattt tgtctgtaag tcttgggtat ttggagcatg cacccatgta gtcgctccgc
     6301 atagaaatgg taaggtaaat cgggacctca tcctgttcga cggcttttag taaaaggttg
     6361 acaaattcag ttgattcatc ttcggattca gcaattcttt cttcttcacg atatctaaaa
     6421 agctcttcaa actggtcaat cataattaac aagttttcac ctgttgcaag attgtgcctc
     6481 cgtgcaatgt cgattagacc tgtattgctt cctcttaata ctgaagaaac aatggttttg
     6541 ttaattattt tctcctcttc acttgcggac tgatattccg gatctttaga taagagtgcc
     6601 tctgcaagat tatcaattgg ggctattcca ggtcttgaaa taataacatt ccaattcggg
     6661 ctcttttccg taacaaatcc accaaataaa acaggcacca aaccacaatt aagcaaggaa
     6721 gatttcccac tgccggaata acctaaaatt gcagcaaact tgttttccgc cagtttttgc
     6781 aagatttcat caacctggct ttcccgacca aaaaagagat gacactcatc aattgagaaa
     6841 ggcctgagcc ctggaaatgg gtttgacagc tttaagtctt cccttatttc cgcctcaagg
     6901 acttcagcct cacgaggctc catatggatt tcgtaattga gcatttcagg tttagtacaa
     6961 tctttaaggg ctgctagagc cgcaaattag taaattatat atgaaaaata caaactttta
     7021 acaatacctt ataattatta tcccccattc ctcagcctgc ataaaggtat cttataacct
     7081 gattggcaaa attgaaattg tcggaaagtt gttcataaaa tttttcctta cttattttaa
     7141 acaagagtgc cttttcctgg gctattaaca agcttgagtc agcatcttcg ctatccgcaa
     7201 gttctccaat aaaatactgt ctcctcatat tacgtacttt ctttccatca gaataataat
     7261 ttacttcacc ggaataaatt atgaagaagt aatcactatt gttaccctgc aaaggataac
     7321 ttgttccttc atgcattgtt acttcctcta tatagtcaac caagttggca agtatacggc
     7381 caggaacttc attgaagatt ctcatattct tcagaaattt gactttttca agttttagaa
     7441 tttttttatc attctctttt gtcccaatta cggtttcaga tataagtctt ttaaattccg
     7501 gattcaagcg gctgatgttt tcatgaaata gttttgggtc cagatgaaac agtgcccaag
     7561 ctgcagtttc agaaacaagc aaatcaggat tgaagaggtt tgcaattaaa tcaattgagt
     7621 aatcgtgaat tttcatcata gcaatttgct tcaatgcaca ggcttttgac catctgttgg
     7681 tttgattgta gtcgcggtta ataagaaatt ttagaacttc ttttgaacca aggctttccc
     7741 gcggaaagaa tgcttcaagt tttcgtgctt tatcaaatac cggcaggtca tccagtacag
     7801 gaatgatctt ttgtttcaaa tcatctgaaa gaaatacatc gagcaattcg attgcataag
     7861 ttattccttc actggttcgg ctactcaaat tttctttgac tagttgaatt gtttttgaat
     7921 cgtatagcat tgaaagaagc acgtaaatat gttcaatatc atgatggttt tcttcttcaa
     7981 ccgctaatcg gaggtcgcgc ccaatttgat tgtcaggaat ttctccaatc gcggcgaggt
     8041 tccaggcgat atcttcgata tcgttttcaa tagcatattt aattctggtg atttgcctga
     8101 tgcttgcctt aaaattgcaa tcacctaaac tatccagtac ttcagaaacc acaattttat
     8161 ccgggaaatc tattttagcc caaagatttt ccatggcagg cctgcttcca atccttccca
     8221 ggattcttat aatttttgtc attacggtaa cgctttgtcc aactctgtag aatgcggagt
     8281 ccaatgcatt gagcgcattg ttcccaatct gaactaagga atttgcggcg acattgctaa
     8341 gccgcaaata atccagattc tctataatcg cattaagcac ctcatagttg taattcttaa
     8401 cacccgcctt catggcagta atcctcactt tatcatccgg atctccgagt agttcaacca
     8461 gcaaatattt gagttcagta tcttccatgt tgctgattaa ttccgcagca taaagtcggt
     8521 cttgcgggtt ttcggatctg cttaatttgg aaattctctg atatgatatt tcaccggttg
     8581 taaataagtt ttccaggtcc agccctgaaa ccacgcgttt tggcaggtta ataaatctgg
     8641 tgggatcaaa tgctattaca tattcatcag agaccgacaa accccgaagt tcgttcaatt
     8701 ttgtttgcgc ataatcctta atttgttcct gctcatgttt catcagggaa tttactgaag
     8761 gagctattga attggggtca attttttcaa gtaatctaaa cgaaaaaact gctttctccg
     8821 gatcggcatg atacaattct ttctccagtt cattaacaac ctggtttcgt ggtttaagta
     8881 atttctccgg atcaatttcg tgactttcta atttgatttt cactctatta cgataccctt
     8941 cataaagttt cactataata tacatccaac cccctatcac tacaatcagg aagtaggagt
     9001 aatgaattag ttcaaagaag ctcaatagcc caagcagaat aataaatcca cctgcaatga
     9061 attttgcaaa ctcattcaca acgccctcaa ctttagcctg gatatcgaat cgaattcgat
     9121 tatccagagg catgaaataa agtttatagg taggattttc cagtgaatcc cttagaaaag
     9181 acgtgaacaa tttactaagt gcaatgaaca aaaagaacca gaagaaattt gttgtattag
     9241 ccgcatcggg attgtagcca aagaatgaac cggccaggat tgaagtaacg gtgaaaaaag
     9301 tcagtacgac tggaagaata aataatgata ccttcaatcc ataatttgaa attattttat
     9361 cattgaaaaa ggtttgaaaa acaaaactga gaatataaat ggaaccgtaa aaccaggcga
     9421 tgaattcctg caattcagtt ccgctagact cagcatattg tgtactcaaa acgttaagaa
     9481 aggaattttc caccaggtat agcgaaatca ccgagaaggc gaggaacaag gaaagcagga
     9541 tgacataatt attctttacc aattccataa atttggtttc tttggtcttc ctttgatctg
     9601 gccgattcac caggttaagg tcaaattttc tagtgatcag tatgaggaaa atcaaggatc
     9661 ctattacgct atagaatcca attacaagga ggtcttcagt gcgacttaat gcagcaattc
     9721 ctgagcctat tgtaaaaaat gtcaaaatct gagcagtaag ctgacctgta tcaattcctc
     9781 caattattct ttttgattgc cttagatcaa ataatcttcc gaagattccc cagaatccca
     9841 gcaaagttat ggctgccaat ggcccttgca gaacatacaa aaggaataca agtattcccc
     9901 ggtattggtc agagtagttt ataagtgcat aatataaggc tgccgaaatt gataatataa
     9961 taatccaatt cagtattgcc agtcttgaaa acgaaattct attttggaga taaacgaaaa
    10021 gtgcggtgga acagacgcca aggaatcctg agacgaagat accctcgctg atgtattctt
    10081 ccatttcacc aacaaacaag gtatctgatg ctatcttgta agttgcaatg aaaattccca
    10141 taaaaaaccc attaccgagt aataagataa cccggcctct ctcttcaggg agaacattaa
    10201 gcgcccggta caataagttt aacatggttc agtcagatga gttctaaaat ataataaaac
    10261 aaatccaaaa ttatgaacaa aacaaggttt gatgcattgc caatggtgga atttcaggtt
    10321 tggctcagtt taaatgataa tgtttccatt tccaaagtta tgtgtaatac tagagtcata
    10381 tttcgtgggt gtgctgtaaa tacattttct ttgcaacatg attattgtca gaagcgcaaa
    10441 gctgatattg ttgtggttcc taatttttgg ttctttttca ggagttttct cacaaaatta
    10501 ttatgatcaa aatttcagga agggatacta ttaccttgac gtgagcattt cccaagctgc
    10561 tgaatatttt ggaaaggcga ttatgaatga ttcaactcaa cctgaagcat acttctaccg
    10621 ggggctggcc tattacaaga tggatgaatt cggtaatgca atcagagatt ttaaaaagtc
    10681 tcttgaaata gatacaacac ttgtgaaaat taatgtgttc atttcattcg cataccgggg
    10741 gttgaatgat tgggataagg ccctggagag tttcaatgaa tatatgatcc gcacttcaat
    10801 agattcaact tctttcaact ggctaatgag aggcaagcta aaacaactga atggggatat
    10861 aaccggtgca ttggaagatt tcaagaaggc agtttttctt agcccggaac aggaaaagtt
    10921 tagattttac cttttcctga ccgagctcga acaaaaaaac tttcaagcgg ctctggaaca
    10981 aattaatgaa atgataaatc ttaatccgga attttatgga tactactttt acaaaggcaa
    11041 catatatttc caaatgatga aatttaatga agccattgaa aattataccg tgtcaattga
    11101 aaaaaataat aataatccgg atggatactt tcgcagggcc tggtctgcag acaccttgag
    11161 taattatact actgcacttg cagactatgg cagggctata gaccttaaat cagatgacgc
    11221 ggcttattac gcaaaaaggg gtaacctgct ttacagaatg gccaaaattg atgatgcttg
    11281 taaagattgg gctgaagcga acaagttgca ttattataaa gattacgaaa agttaaataa
    11341 actttgtaaa taaattatta acctaaacaa atacaactat ggataatcgt aaatatttgc
    11401 ccatattaat tgtgggagga atcgcgttaa tagtaattct actattatcg aacagcattt
    11461 ttttaacaat tgaacccggt aaagcggggg tactattcaa aaaatttggt ggtggccttg
    11521 ataaagaacg gatctattat cagggatttc aggtaattgc tccctggaat acaatgttta
    11581 tttatgatgt tcgtgaacag acggctgagg aatcaatgga cgttcttgac aaaagcggat
    11641 tgtctataag tgttgatgta actgtcaggt tcaatccaca acctgttaag attggattta
    11701 ttcacgaacg atttggtatt gattataaaa atacgctggt aattccggaa gtaagatcga
    11761 cagtaagaca ggtaatggga aggtttacag cggaggaaat ttacagtacc aaacgggccg
    11821 aggttgaagg agccataaaa acagaaactg aaaacgtttt gggaagtgta aacaataata
    11881 tcgttatgaa cgcaatgctc atcagatcta ttaatttgcc ggaacaaatt aagatggcca
    11941 ttgaaaataa attaactcag gagcaagaag cacttgctta ccgattcagg ctggataagg
    12001 aggagagtga ggctaaaaga aagcagattg ctgccgaagg tgaggcacgc gccaaccaga
    12061 ttatcaatag tagtttaacg cctagtcttc ttaaaatgag aggtatagaa gcaacaacca
    12121 gtctcgccag cagtcctaat tcgaaaataa ttgtaattgg cagtggttct gatggcttgc
    12181 ctcttattct tggcagtaat taagcttcat taaaggggtt cagtcatttt attttcactt
    12241 gttttttggt tatcttggct ggcctctaaa atgaaaaaaa acatataatt aatatacata
    12301 aaatgtctga tcaagtagaa ttaaaaatca aggataagaa ttaccagttt cctacaacga
    12361 agggttctga agatgaaatg gcgattgata tcagttccct ccgaagtcag accagctaca
    12421 ttacgttgga tcccggcttt aagaatacgg gatctactac cagtgcgatt acatttctgg
    12481 atggagaaaa ggggattctc aggtataggg gttatccaat agaacaattg gccgaaaagt
    12541 caaactttct tgaggtctcc tatcttttga tttatggcga attacccggt aaggcggagt
    12601 ttgaggaatt caggaagcaa attacccgaa gaagtctcgt ccacgaggat attaagaata
    12661 tcctcatggg ctttcccagt gctgctcatc caatgggagt attatcctcg ctggttagtt
    12721 ctttaactgc tttctatccc cagtcactcg atccacatcg tggaaaggat aaaattaatt
    12781 tgagcataat aagactgctt gcaaaatttc ctactatcgc ggcctggacc tacaagaata
    12841 agattggtca aacatttaat tacccggata acagcaagga ttattgtgaa aattttattg
    12901 aaatgatgtt caaggttcct actgaacctt ataaacttga tccggtggtt gcctcagctt
    12961 tggataagct gttaatcctc catgctgatc atgaacaaaa ctgctctaca agtacagtgc
    13021 gactcgtagg ttcttcccag gcatctttgt actcttccat ttcagcaggg atttgtgcat
    13081 tgtggggacc attgcatggt ggagcaaacc aagctgtaat tgaaatgctg gagaacatca
    13141 aggaagatgg cggagattat aagaagtgga tgcagaaagc aaaagacaaa gatgatcctt
    13201 tcaggttgat gggatttggc cacagagtgt acaagaattt tgatcccagg gcaaaaatca
    13261 ttaagggggc tgccgatggt gtactggata gcttgggcat caaggacccg gtgctggaga
    13321 ttgccaaagg acttgaagaa gtagccttga gcgattctta ctttgtggag agaaaattgt
    13381 atcctaatgt ggatttctac tctgggatta tttacagagc attgggaata ccggttgagt
    13441 tctttacggt gatgtttgca ttaggaagac tacccgggtg gattgcacaa tggaaagaaa
    13501 tgattgagaa taccgaaccc attggcagac caaggcaaat ctacattgga gccacagaaa
    13561 gagactacat tgattacacc aaacgataat tgaaaggggc cttcgggcct tttttaataa
    13621 ctaaactg
//
LOCUS       DASWHX010000163        14791 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_553175, whole genome shotgun sequence.
ACCESSION   DASWHX010000163 DASWHX010000000
VERSION     DASWHX010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 14791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14791
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_553175"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(46..477)
                     /locus_tag="VGA21_10930"
     CDS             complement(46..477)
                     /locus_tag="VGA21_10930"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX9651067.1"
                     /translation="MNRLTGLFLLAVFFSCTQSNPNLSREEEIKWKQYRIKGRSLYQL
                     HCSNCHGINGEGLVNLYPPLSNVDWVTLNSEKISCVIKNGIQGELKINGTLFSGDMPG
                     HNNLTNLEIAEITAYILSDLNESQEFYDVNEIIRQLNACED"
     gene            complement(470..1117)
                     /locus_tag="VGA21_10935"
     CDS             complement(470..1117)
                     /locus_tag="VGA21_10935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020530562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEX9651068.1"
                     /translation="MTRIFAVSVVLVFLTLCTAPEKKLPILGYRDVNMVEKNGETIAD
                     TIYHTIGDFRVHNQDGNVITNDTFSDKIYVADFFFTTCPSICPVMTAQMLRVYDHYQT
                     NDKVKLVSYTIDPKHDSVSVLKEYANKLEVSSNTWHFVRAGKDSIYNLAEKSYMVRAA
                     EDQFAPGGFIHSGAFALVDPKRRIRGLYDGTRPEDVDRLIAEIRILLKEIEMTNE"
     gene            complement(1133..1930)
                     /locus_tag="VGA21_10940"
     CDS             complement(1133..1930)
                     /locus_tag="VGA21_10940"
                     /inference="COORDINATES: protein motif:HMM:NF015438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC N-terminal beta barrel domain-containing
                     protein"
                     /protein_id="HEX9651069.1"
                     /translation="MATSNMERKVKSLHIYPVKSLGGISVAFSQVTSSGLQFDRNWML
                     IDEAGNFMTQRKFPEMASFAIKVSDHSLEISNHNDTILAPFADSGPELETKVWGDKIQ
                     GKEVSEETSNWLSEKFGFRVKLIHFATLRKVPDAPGTEIPFSDSSPFLLIGEPSLLDL
                     NTRMKDNLSITRFRPNIVVSGFDPYEEDTWHRIRIGSCRFQVFKKCARCQLPNVNPET
                     AKVDKSILKFLSTYRKDEINRINFGVHLILIEGKVINVGDEIELLSS"
     gene            complement(1900..2514)
                     /locus_tag="VGA21_10945"
     CDS             complement(1900..2514)
                     /locus_tag="VGA21_10945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002705402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04282 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HEX9651070.1"
                     /translation="MEKENCLIIFVKNPALGKVKTRLAEEIGDGPALAVYLKLVNHTR
                     SVTQNLSGNKIVYYSDFVDMEDEWENELFRKSLQSGSDLGIRMKNAFSDQFNSGYKKV
                     CIIGSDLPELSGEIINNAFSILETSDAVIGPAHDGGYYLLGMKKLIQKVFENKEWGNH
                     RVFESTMENFRSLNLTCHQLSTLLDIDTKNDLEKAWPQVTWREK"
     gene            complement(2493..3812)
                     /locus_tag="VGA21_10950"
     CDS             complement(2493..3812)
                     /locus_tag="VGA21_10950"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HEX9651071.1"
                     /translation="MSTQYRTTLLVFLLSIPVYIILHYYTMRFESIQLITGYAFLFFL
                     YLCILKIAGSEDILKTALLFAVLYRILVIPGLPSLSDDFYRFFWDGKLIALGINAWQY
                     TPAEIVARVPNSMDAGLFNLLNSPDYHSVYPPPLQGLFFAAVYLSPESIIGSTVILKL
                     VILLAELFTILLLLKICDRWELKRSNVLIYALNPLVILELTGNIHFEALAIPFLLFAV
                     LLYSKEKDFFSGIALGLAASLKLLPALFAPLFISHFQGKKIFYWSTGFILVSFLSLFP
                     LLKNFDSGIGSGLNLYFQKFEFNASIYYLVREAGFWLKGYNTIATIGPWMAFATVLFI
                     FILSYLSFKKKIQFQNSLLFALTIYLLMTTTVHPWYIIPLIAITTLTNYRFPVIWSCL
                     IFLTYTGYSHNSFTEYSAVVFTEYFITFGFAVWEVRKYSSNWKKKIA"
     gene            complement(3830..4525)
                     /locus_tag="VGA21_10955"
     CDS             complement(3830..4525)
                     /locus_tag="VGA21_10955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015028831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX9651072.1"
                     /translation="MNPITRVIIPAINEENSIAKVIAGIPKSLVSEVIVSDNGSSDNT
                     PEIARQAGATVLIEPAKGYGNACLKAIEYVKSLEEKTDIIVFLDADFSDYPEEMTKII
                     VPIIEADLDLVIGSRALGAMQKGSMTFPQIFGNWLSTRLINLFFGIKFTDLGPFRAIK
                     YDKLLQLDMQDKNFGWTVEMQVKAAKKKFRCVEVPVSYRKRIGASKISGTIMGTVLAG
                     HKILWTIFKNLLD"
     gene            complement(4540..6321)
                     /locus_tag="VGA21_10960"
     CDS             complement(4540..6321)
                     /locus_tag="VGA21_10960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002693708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX9651073.1"
                     /translation="MKHLRLTGLSLFISGFILFILTFFAGNYKLTGEILNATVKDDAQ
                     FKLLETGLSPLYNQEFSNSFQFVGKLNAAFREINNKQIATYEITGSEADQIVQLNETQ
                     LNAETLASVFAENDIGQYKRKVFLQYGGWLIQASFSSAEEFRTAVEKVANDIRKYAIM
                     NDKGFSKYAIRDLDFQLTKASSFGIITRSPSMAMFIIYGLCILGGLLMIIPVYLSEPP
                     GIKNNGIYKNPMKNRGWLGILTGSFLILFYIFLYFYPEHMTQWVIMVDPLSYFLNGDQ
                     AGRFFLYGFLYTLCVLVMGIRMILKYRHSRYHLIRTFSVMFFQLAFAFLIPEFLVRLN
                     QPFFDFKNIWPLDYDFFFDSEIIKLVQSGSIGLFMLFWGILLAIIVVPLFTYLYGKRW
                     YCSWVCGCGGLAETLGDPYRQLSDKSLKAWKIERWMVHGVLAFSVIMTIGVIYTYFTG
                     SGNLLGINTYSLREIYGVWIGAGFAGVVGTGFYPLMGNRVWCRFGCPLAAYLGLIQRF
                     KSRFRITTNGGQCISCGNCSTYCEMGIDVRWYAQRGQNIVRSSCVGCGVCASVCPRGV
                     LKLENGSLKNRIPDPIEIGKNGLTLNS"
     gene            complement(6318..7631)
                     /locus_tag="VGA21_10965"
     CDS             complement(6318..7631)
                     /locus_tag="VGA21_10965"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009580223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HEX9651074.1"
                     /translation="MSQRIVIIGNGIAGVTAARFIRKKSNYSITVISSESEYFFSRTA
                     LMYVFMGHMKFEHIKPYEDWFWKKNKINLAFDIVKSISFSEKKVTLGKGEIIAYDKLI
                     IATGSKSKQLSCSGENLNGVHTLYSLQDLARLDESISGKSNAVIIGGGLVGIELAEML
                     HSRGIRVEFLIREDGYWRDVLALEEAELISRHIKKYGITLRCNTQVKAFIGNSTNEVE
                     SVLSNDDQNITCDLACVAVGVTPNVDFLRNTELKIESGILVNQYLETSIPDIFAIGDC
                     AQIINPDSGRKKIEPVWYAGKFMGEVVANTVCGNSMEYHPGNWYNSAKFFDIEYQVYG
                     YVPNEKTSETEWFYWEDMGTEKSMRLVWSKNNNSFLGIQTLGIRISHPVIDSWINKKI
                     KIGQVIMDLEKINFDPEFFTRHEKKIRNQFKKEFPELMRSKELTT"
     gene            complement(7790..8788)
                     /locus_tag="VGA21_10970"
     CDS             complement(7790..8788)
                     /locus_tag="VGA21_10970"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9651075.1"
                     /translation="MTSIQNDIVLSTENLSIGYPEHGTPVLSNLNLSLRKGTLVSFMG
                     PNGIGKTTLLRTLAGLMNPLDGKIWIRNKEIKNYSLNQLAKIIGVVLTDPIQAGSLSV
                     TDLVSMGRYPYTNWFLNFSESDIEKVEQAIYSVQLEGLRNRKINELSDGQMQKVLIAR
                     ALAQDGEIMILDEPNSHLDLNNRIEIMRILKRMCRQTGKTILVATHELDLALQTSDWL
                     WLATRAGKIIDGIPEDLVLNNAFDEVFDTKGFDLKTGRVIHEGEQLAEVTLQGTGFMY
                     LWTKNALERTGIQIAESSEIRITINEDLQKWTIQLPQGTKEVNSISELIESILSGN"
     gene            complement(8785..9834)
                     /locus_tag="VGA21_10975"
     CDS             complement(8785..9834)
                     /locus_tag="VGA21_10975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019945036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEX9651076.1"
                     /translation="MNKPIQKNISLLWVLLLAILVLLFFIDIFIGSVYIPAAEILSIL
                     TGGESTQESWYHIITKFRLPRVITAILAGSSLALSGLFMQTYFRNPLAGPFVLGISSG
                     SSLGVALLVLSGGMLGGVFAGLGTNQWIIMLAASAGAGLVLLLILIASTRIKDSMTLL
                     IIGLMFGSATGAIVGILQFFSNAEEIQLFILWTMGNLSSLSWPELHVFLIVFGVCVLL
                     SFTLFKTLNSLLLGENYARSMGIPLKRTRFLIIAVTSLLAGCTTAFCGPIAFIGIAIP
                     HIARMLINTSQHKLLVPATIFSGAIILVLCDIISQLPGSQNILPINAITSLMGAPVII
                     WVILRRKNYQKTFAG"
     gene            complement(9835..10926)
                     /locus_tag="VGA21_10980"
     CDS             complement(9835..10926)
                     /locus_tag="VGA21_10980"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9651077.1"
                     /translation="MNTFLGCNNAEQAKVIESSHGTIKYARGFNIESYPGYKKVTITR
                     LFKGSTSEKVYYLTRDDKNDIPQNGIRIQVPVSKMVCSSTTHLPALVLLNEVNSLAGF
                     PSVNYISSPEIRSRIETGNVRDLGSENGLNLEGIVELSPDLMMGYLLTGDSKLYDKIE
                     SSGVPVVINSDYLEETPLGRAEWLKFTAALFNKEEMADSIFSAIEKEYLAIKDLAGKV
                     NYRPTVFGGVMYGGTWFMPGGKSFAGIFLEDAGGNFLWSDTAGSGSLELSFESVLEKA
                     HDAEYWIGASNFKSKADMSQSDIRYELFQAFKQDRIYTYTLNVTVEGGNDFFETAYAR
                     PDWVLSDMVKIMHPDLLKHKSLHFFQKID"
     gene            complement(10967..11962)
                     /gene="meaB"
                     /locus_tag="VGA21_10985"
     CDS             complement(10967..11962)
                     /gene="meaB"
                     /locus_tag="VGA21_10985"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015030717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HEX9651078.1"
                     /translation="MYHKTKNRETSAYYIDGILKGDIPILSQAITLTESHLPPDQNIA
                     GEVLEGILERTGNSIRIGITGIPGVGKSTFVESFGTHLTNLGKKIAILAVDPSSKFSG
                     GSIMGDKTRMEQLARNPAAFIRPSPTGSNLGGVAAKTRESILLCEAAGFEIILVETVG
                     VGQNELAVREMVDFFLLLMLARSGDELQGMKRGIMEMANLIAITKADGDNVEAANQAR
                     AQFHSALHLYSMPESDWKPKVITCSAFESKGLSEIWEAICEYENITRENDFWNIQRNR
                     QLLQWFHSTMEWMLLENFKTSKGIPSIIKEMEKKIMNKSITPTRAAGEIVDQYNK"
     gene            12043..13344
                     /locus_tag="VGA21_10990"
     CDS             12043..13344
                     /locus_tag="VGA21_10990"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HEX9651079.1"
                     /translation="MIFKQKSYWIDVVFGTLFIFSIMFALGSLLDKLEFIDPIGDALE
                     DMEITDRVFSDPDLREILDADTNIVLVNIGLLSRGEISEQINIINKYQPKVIGLDAVF
                     TSLKSDTLGDLMLANALANTPNIIMYGKLIEPDNDEVWNGMELSHPIFTQDHEIAHVN
                     LVTESAGTEQFQFKTCRSFFPMEKLRDPETNLITDVPALAVKICEIYNPEVTAGFLER
                     QIDEEIVNYRGNIMDFGLTKFGGRFYALDVDDVLNENFDPSLLKDKIILMGFLGQYIG
                     DPNTVEDKYFTPMNSKFAGRALPDMFGVVIHANIASMILNEDYMNQMTEMGSALFAII
                     VCILNVAVFQIIYIKMDRWYDGLTKLIQLVEAVLLLFVIVLVFHYYSFKLDLTYGIIA
                     ILLAGDSLEVYNGVVKNIFRKEKKKKPLTIPEEKVSIGNTN"
     gene            13360..14259
                     /locus_tag="VGA21_10995"
     CDS             13360..14259
                     /locus_tag="VGA21_10995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651080.1"
                     /translation="MRTLLSIGVGLLLSTAIFAQDYEFRVMMNKGENQIKSSGGDWEA
                     LKSGTRLNKGDELKLASGAYLGLVHKSGKTMELKEAGTHKIDDLASKIGVGNTSITSK
                     YADFVMAKMTEDSKEENRRKYASVTGAVERAADDASIKVLMPTSVDVYTTNPILAWNP
                     IEENKYKVVVKNWFDKTLDTYETTESWIRLNLEDPKLAKEAMLVVSVSLANNETVNSG
                     DYGLKKLTEADALDFSEELTELKSALTEESSLNRVILAEFFEQNNLLVDALTNYVIAI
                     QLSPNVDYFKEAFEEFKLRNGLK"
     gene            14520..>14791
                     /locus_tag="VGA21_11000"
     CDS             14520..>14791
                     /locus_tag="VGA21_11000"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEX9651081.1"
                     /translation="MKKIYHILTMMIAVAVFAACSSTANTGTTTAKKEKDYSNLSSLA
                     DVLRTVGGIQVSGVGSDVTVSIRGQQTIMLNTQPLYVIDGLPIGND"
BASE COUNT         4480 a         3061 c         2913 g         4337 t
ORIGIN      
        1 gaaaatcaaa caagtcatgg aagccggcag gtccgcgatc aacaattaat cttcacaggc
       61 atttaattgc ctgatgattt catttacatc ataaaactcc tgactttcat tgaggtcaga
      121 taaaatataa gcagttattt ccgctatttc caaatttgtt aaattattat ggcccggcat
      181 atctccgcta aacagggttc cattaatctt taactcgccc tgaattccat tcttaataac
      241 gcaggaaatt ttttcagaat taagtgttac ccaatctaca ttactcaatg gaggataaag
      301 attaaccaat ccttctccat taattccatg acaatttgag caatgaagct gatacagcga
      361 ccggcctttg attctgtatt gtttccattt tatttcttct tccctggaaa ggtttgggtt
      421 tgattgtgtg caagagaaaa atactgccag cagaaaaagt ccggtgagtc tattcattcg
      481 tcatttcaat ttccttcagc aggattctga tttcggcaat cagccggtct acgtcttccg
      541 gccgcgtgcc atcatacaag ccccggattc ttcttttcgg atctaccagg gcaaatgcgc
      601 cactgtgaat aaaacctccc ggtgcaaatt gatcttccgc tgcccgcacc atataacttt
      661 tttccgcgag attgtaaatc gaatctttgc cggctcttac aaagtgccag gtattggagc
      721 tcacttccag tttgttagcg tattccttta gcactgagac agaatcatgt ttgggatcta
      781 tggtgtaact caccaacttc actttatcat tagtttggta atgatcatac acacgcaaca
      841 tttgagcagt cataacggga cagatagaag ggcatgttgt aaagaagaaa tccgctacat
      901 aaattttatc cgaaaaagtg tcattggtga ttacattgcc atcctggtta tggaccctga
      961 aatctccaat ggtatgatag atcgtgtctg caatagtttc tccattcttt tccaccatgt
     1021 taacatccct gtagccaagg atgggaagtt ttttttccgg cgcggtacac aaagtcagaa
     1081 aaacaagaac aacagaaact gcgaagatcc gggtcataga tcaaaaaata tttcaggagg
     1141 atagaagttc aatttcatcg cctacgttta ttacttttcc ttcaatcaaa atcaaatgta
     1201 cgccaaaatt gattcgatta atttcatctt tccggtaagt ggaaaggaac ttcagaatgc
     1261 ttttatccac ttttgctgtt tcaggattta cattaggcaa ctgacatctg gcgcattttt
     1321 taaaaacctg aaaccggcag cttccgattc taatccggtg ccatgtatcc tcttcatagg
     1381 gatcaaatcc ggataccaca atgtttggcc tgaagcgggt gatggaaaga ttatccttca
     1441 ttcttgtgtt gagatcaagc aatgaaggct cacctataag gaggaatggc gatgaatcgg
     1501 aaaaaggaat ttctgttccg ggggcatccg gcacttttct caaagttgca aagtgtatta
     1561 gttttaccct gaaaccgaat ttctctgaaa gccagttact tgtctcttca gaaacctcct
     1621 taccctggat tttatcaccc cagactttcg tttcaagttc gggtccggaa tccgcgaacg
     1681 gtgccaggat agtatcattg tgatttgaaa tttccaatga atggtctgaa accttgatcg
     1741 cgaaggaagc catttcagga aattttcgtt gtgtcatgaa attgccggct tcatcaatca
     1801 gcatccagtt tcggtcaaat tgcaagcctg atgaagttac ctgggaaaat gccacagata
     1861 ttccacccag gcttttaacc ggataaatat gcagtgattt tacttttctc tccatgttac
     1921 ttgtggccat gctttttcca aatcattttt tgtatcgatg tccaataatg ttgataattg
     1981 atgacaagtt aaattaagac ttctaaaatt ttccatggtc gactcaaaaa cccgatggtt
     2041 tccccactct ttattttcga aaactttttg gattaacttt ttcattccca ataaataata
     2101 accaccgtcg tgggcagggc ctataaccgc atcgctggtt tccaaaatgc taaatgcatt
     2161 gtttataatc tccccggaaa gttctggtaa gtcgctgcca atgatgcaaa cttttttgta
     2221 tcctgaatta aattgatctg aaaaagcatt cttcattcta attccaagat cactcccgga
     2281 ttgaagggat ttcctgaaaa gttcattttc ccattcatct tccatgtcca caaagtctga
     2341 atagtagaca attttgttac cggatagatt ttgcgttact gaacgagtgt ggttcacaag
     2401 ctttaaatag acggcaagtg ccggcccatc tccaatttct tccgccagcc gggttttcac
     2461 tttgccgagg gcagggttct taacaaaaat aatcaggcaa ttttcttttt ccaatttgaa
     2521 gaatattttc tcacttccca cactgcaaac ccaaaggtaa tgaagtactc agtaaataca
     2581 accgcggagt attcagtaaa gctattatgc gagtaacctg tgtaggtgag gaagatgaga
     2641 caagaccata taacggggaa ccgataattt gttaaagttg taattgcaat aagcggtata
     2701 atataccagg gatgaacggt ggtcgtcatc aacaaataaa ttgttaaagc aaaaagtaag
     2761 gaattctgaa attgaatttt ctttttgaat gagagatagc ttagaatgaa aatgaaaaga
     2821 actgttgcga atgccatcca gggaccgatg gtggcaatgg tattatatcc tttcagccag
     2881 aacccggctt cacgcactaa ataataaata cttgcattga attcaaattt ttgaaagtag
     2941 agatttagcc ctgaaccgat tccggaatca aaattcttca acaatggaaa caggcttaga
     3001 aatgaaacca ggatgaaacc cgtgctccaa tagaaaattt ttttcccttg aaaatgagaa
     3061 ataaacaatg gcgcgaacaa tgcgggcaaa agttttaatg aagcggctaa tcccagagcg
     3121 attccggaaa agaaatcttt ttcttttgaa tataacagaa cggcgaacaa caggaatgga
     3181 atagctaaag cttcaaaatg aatattaccg gttaattcaa ggataaccag tgggtttaag
     3241 gcatagatta acacatttga tcttttcagt tcccatcgat cacagatttt caataacaat
     3301 aaaatggtaa atagctctgc aagaaggatc accagtttca gaataacggt gctgccaata
     3361 atggattccg gtgagagata gacagccgca aagaataagc cctgcagcgg gggtggatat
     3421 acgctatgat agtccgggga atttaacaga ttgaacaggc cggcatccat agaattggga
     3481 accctggcca caatttctgc tggtgtgtat tgccaggcat taattccgag cgcaattaac
     3541 ttaccatccc agaaaaaccg gtagaaatca tcggaaaggc tgggtaatcc gggaattacc
     3601 agtatcctgt ataaaacagc aaacaataac gctgttttta ggatgtcttc actacctgca
     3661 attttaagaa tgcagagata aaggaaaaat aaaaatgcat agccggtgat aagctgaatg
     3721 ctttcaaacc gcatggtgta gtaatgaaga ataatatata ccgggatgga aagtagaaat
     3781 actagcaggg tggtccggta ttgagtactc attgaaatct cttaataaac taatcaagga
     3841 ggttcttaaa gatagtccat aaaattttgt gacctgccag aacggttccc ataatggttc
     3901 cggaaatttt ggaggctccg attctttttc ggtaggatac aggaacttcc acgcacctga
     3961 acttcttttt ggctgcctta acctgcatct ccactgtcca cccaaaattt ttatcctgca
     4021 tgtcgagttg cagcagttta tcatatttga ttgcccgaaa ggggcccagg tcggtaaatt
     4081 taattccgaa gaaaaggtta attaacctgg ttgacaacca gttaccgaag atttgaggga
     4141 aggtcattga tcccttttgc atggcaccca gtgcccgtga tccaatcact aaatccagat
     4201 ccgcttcaat tatcggtaca ataattttgg tcatttcttc cgggtaatcc gagaagtcag
     4261 cgtcaagaaa aacaatgatg tccgtttttt cttccaggct tttcacatat tcaatggctt
     4321 tcagacaggc gttaccgtag ccttttgctg gttcaattaa gacagtagct ccggcttgcc
     4381 gtgcaatttc aggagtatta tcagaggagc cattatcaga aacgattact tcgcttacta
     4441 aactttttgg aattcccgcg atgactttgg caatggaatt ttcttcattg atagcgggaa
     4501 taattacgcg ggtaatgggg ttcattcagg agagtggatt cacgaattaa gcgttaatcc
     4561 atttttgccg atttcaattg gatccggtat tctgtttttg agtgatccat tttcaagttt
     4621 caatacgccg cgcgggcaaa cactggcaca aacaccacag cccacacatg aggaacgcac
     4681 tatgttttgt ccacgctggg cataccagcg cacatcgatt cccatttcac aatatgttga
     4741 acaattgccg caggaaatgc actgtccgcc gttggtcgtt atccggaacc ttgatttaaa
     4801 tcgctgaatc agaccgaggt atgcagcaag cggacaccca aagcggcacc acacgcggtt
     4861 acccattaaa ggataaaagc cagtacccac cactccggca aatccggcgc caatccagac
     4921 accataaatt tcccgcaatg aatacgtgtt gatacccaat agattgccac tgccggtgaa
     4981 gtaggtgtat ataacaccga tggtcatgat tacagaaaat gcaagaacac catggaccat
     5041 ccatctttca attttccagg ccttcaatga tttatctgaa agttgacgat atggatcacc
     5101 taaagtttca gcaaggccgc cacatccgca aacccaggag caataccacc gtttgccgta
     5161 taaatacgta aacagcggaa cgacaattat tgcaagcaaa attccccaaa aaagcataaa
     5221 taatccaatg gagccagact gcacaagttt gataatttca ctatcaaaga aaaaatcata
     5281 atccagtggc cagatatttt tgaaatcaaa gaagggctga ttgagccgta ccagaaattc
     5341 cggaatcaga aaagcaaatg ccagctggaa aaacataaca gaaaatgtcc ggataagatg
     5401 atagcgcgaa tgccgatact tgagaatcat ccggatgccc attactaaaa cacaaagcgt
     5461 gtagagaaaa ccgtagagaa aaaaacggcc ggcctgatct ccgttaagaa aatagctaag
     5521 gggatcgacc ataataaccc attgggtcat atgttccgga tagaaataaa gaaatatata
     5581 aaatagaatc aggaaggaac cggttaaaat tcccagccag cccctgtttt tcataggatt
     5641 tttataaatc ccattattct taattcccgg tggctctgat aaatagacgg ggatgatcat
     5701 caataaacct ccgaggatac acagtccata aataataaac attgccatgg aaggagaacg
     5761 ggtgatgata ccgaatgaag aagctttggt caattgaaaa tcaagatcac gaatggcata
     5821 cttactaaat cccttgtcat tcataattgc atatttgcga atatcatttg caaccttttc
     5881 aaccgcagtt ctaaattctt cagctgaaga aaaacttgcc tggataagcc agcctccata
     5941 ttgcaaaaag acttttcgct tatattggcc aatatcattt tctgcaaata ctgacgcgag
     6001 agtttccgca ttaagttgag tctcatttaa ctgaactatt tggtcagctt cactaccggt
     6061 gatctcataa gtagcaattt gtttattgtt gatctcccgg aaagctgcat taagtttgcc
     6121 aacaaactga aaggaatttg aaaactcctg attgtaaagc ggactcaatc cggtttcaag
     6181 caatttgaat tgtgcatcat ctttaactgt tgcgttcaat atttcgccgg tgagcttgta
     6241 gttgcctgca aagaaagtaa gtataaacag gatgaagcct gaaataaata gtgagagtcc
     6301 ggttagccgc agatgcttca tgttgtcaat tcttttgaac gcattaattc cggaaattct
     6361 tttttaaatt gatttcgaat ctttttttca tgcctggtaa aaaactcagg atcaaaattg
     6421 attttttcaa ggtccataat tacctgacca atctttattt ttttattaat ccaactatca
     6481 ataaccggat gtgagattcg aattcccagg gtttgaattc cgagaaaaga attgttgttt
     6541 ttagaccata ccagccgcat ggatttttcg gtgcccatgt cttcccaata aaaccattca
     6601 gtttctgaag ttttctcatt aggtacatac ccatagacct ggtattcaat gtcaaagaat
     6661 ttagctgagt tataccagtt tccgggatga tattccattg aatttccgca aacagtattg
     6721 gcaacaactt cacccataaa cttgccggca taccaaaccg gttcaatttt ttttcttcca
     6781 gagtcgggat ttataatctg tgcacagtcg ccgatggcaa atatatcggg gatgctggtt
     6841 tccaggtatt gattcacaag aattccactt tcaattttga gctccgtgtt tctcaggaaa
     6901 tccacgttag gtgtaactcc aaccgcaacg caagccagat cacaggtaat gttctggtca
     6961 tcattgctga gtacagattc aacttcgtta gtagaattac ctataaatgc ttttacctgt
     7021 gtattgcagc gaagagttat tccgtacttt ttgatatggc gggaaatcaa ttcagcctct
     7081 tccaatgcca acacatccct ccaatagcca tcctccctaa ttaagaactc aacccgtatg
     7141 cctcgactat gtaacatttc agccaattca attcctacca gtccaccccc aatgattacc
     7201 gcattgcttt tgccagagat cgattcatcc agcctggcca gatcctgaag actgtaaagg
     7261 gtatgaactc cattcaaatt ttctccggaa caactcaact gttttgattt ggatccggtc
     7321 gcaataatga gtttatcgta agcaatgatt tcaccttttc ccagcgttac ttttttctct
     7381 gaaaatgaaa tggactttac tatatcaaaa gccaggttta ttttattttt tttccagaac
     7441 caatcttcat agggttttat gtgctcaaac ttcatgtgac ccatgaagac atacatcagg
     7501 gcagttcggg agaaaaaata ctccgactcg gaagaaataa cggtaatgga atagttagat
     7561 ttctttctga taaatctggc agcggtaacc cctgcaattc cgtttcctat gatgacaatt
     7621 ctttgtgaca taagcaggag tgaaatttag cttttaaatg tgctaatcaa aacctcctga
     7681 actaaaagat tacagtaccg gtgatgaaaa gtttattcag aaggctggca ataaatacga
     7741 aaaaatcgtt gtggttttca agggaaaaat gaactattga ttaaatgatt tagtttcctg
     7801 agaggatgga ttcgatgagt tcgctaatgg aatttacttc ctttgttccc tgaggcaatt
     7861 gaatggtcca tttctgtaga tcttcattaa tcgtaattct aatttctgaa gattcggcaa
     7921 tttgaattcc ggttctttcc aatgcatttt tagtccatag gtacataaat ccggttccct
     7981 gaagggtaac ttcggcaagt tgttctcctt cgtgaataac ccggcctgtt ttcaggtcaa
     8041 atccttttgt gtcgaaaact tcatcaaaag cattattaag caccaggtct tcgggaattc
     8101 catcaatgat ttttccggcg cgggtcgcga gccataacca gtcgctggtt tgcaaagcca
     8161 aatcaagttc atgagttgcc accaggatcg ttttaccggt ttggcggcac atcctcttca
     8221 gtatcctcat aatttcgatc cggttattta aatcaagatg cgaatttggc tcatcgagga
     8281 tcattatttc tccatcctgg gccagggccc tggcgataag cactttttgc atctgaccgt
     8341 cactaagctc attaatctta cgatttctta aaccttcaag ctgtacagaa tatattgcct
     8401 gctccacttt ctcaatatca ctttcagaaa aattcaaaaa ccagttggta taaggatatc
     8461 tgcccatgct caccaaatca gtaacactca aactgccggc ttgaatggga tcagttagca
     8521 ctacgccgat tatttttgcc agctggttca atgaatagtt ttttatttct ttattgcgga
     8581 tccaaatttt accatccagc ggattcatca accctgccaa tgttctcagc agcgtggttt
     8641 tcccaattcc gttgggtccc ataaaactca ccagggttcc ttttcgtaac gagagattta
     8701 gattcgacaa gacgggtgta ccgtgttccg ggtagccaat acttaggttt tcagtagata
     8761 aaactatatc gttttgaata ctggtcatcc ggcaaaagtt ttttgataat tttttcttcg
     8821 tagtattacc caaatgataa caggagctcc cataagagaa gtgatggcgt taataggtaa
     8881 aatgttttga gacccgggaa gctgtgaaat aatatcacaa aggacaagga tgatggctcc
     8941 actaaatatt gttgccggaa caagtaattt atgctgagaa gtattaatca acatcctggc
     9001 aatgtgggga attgctatac caataaaggc aataggtccg caaaaggctg ttgtgcagcc
     9061 tgccaacaga ctagtgacag ctattataag aaaacgggtt cttttcaacg gaattcccat
     9121 cgatcgggcg tagttttctc ccaagagtaa cgagtttaat gttttgaaaa gtgtgaatga
     9181 caacaagacg catactccaa agacaatcag gaagacatgc aactccggcc aggacaggga
     9241 actcagattc cccatggtcc ataaaatgaa caattgaatc tcttcagcat tgctaaagaa
     9301 ctgcaaaatc ccgacaattg cgccggtggc actgccaaac atcaatccga taattagcaa
     9361 agtcatgcta tccttaattc ttgtagatgc aatcaggatt agcaacagaa ctaatccggc
     9421 accggccgat gcagccagca ttattatcca ttgattggtg cccaaaccgg caaaaactcc
     9481 gcccaacatt cctccggaaa gcaccagtaa agcaactccc aaacttgatc cggaactaat
     9541 tcccaaaaca aaaggcccgg caagtggatt cctgaaataa gtttgcataa ataatcctga
     9601 aagagcgagc gatgaaccgg caagaatggc ggtaataact ctcggtaaac ggaattttgt
     9661 aattatgtga taccagcttt cctgggttga ctcaccccct gtaaggatgc ttaaaatttc
     9721 agctgctggt atatatacac ttccaatgaa tatgtcaata aaaaacaaaa gaaccaatat
     9781 ggctaacagc aaaacccata gcaatgaaat attcttttgg atgggcttat tcaattaatc
     9841 aattttttga aagaaatgaa gacttttatg cttgagtaaa tcaggatgca taattttaac
     9901 catgtcggaa agcacccaat cgggtcttgc atacgcggtt tcaaaaaagt cgttacctcc
     9961 ttccacggtt acatttaatg tataagtgta aatacgatcc tgcttaaatg cctgaaagag
    10021 ttcgtatcgt atatcgcttt gagacatatc agcttttgac ttgaaatttg atgcaccaat
    10081 ccaatactcc gcgtcatgtg ctttttcgag cacgctttca aagctaagtt ccaggcttcc
    10141 gcttccggct gtatcagacc aaagaaaatt tcctcctgca tcttccagaa aaattcctgc
    10201 aaaacttttg cccccaggca taaaccaggt tccaccatac atgacacccc cgaaaactgt
    10261 gggccggtaa ttaaccttcc ctgcaagatc ttttattgcc aaatattctt tttcaatggc
    10321 tgaaaaaata gaatccgcca tttcttcttt attaaataaa gcggcagtaa actttaacca
    10381 ttctgccctg ccaagcggag tctcttccag gtaatctgaa ttaatcacta ccggaactcc
    10441 cgagctttct attttgtcat acaattttga atcaccggtc aggaggtagc ccatcatcaa
    10501 atcgggcgag agttctacaa ttccttccag gttcaatcca ttttctgagc ctaaatccct
    10561 tacgttgccg gtttcaattc tggagcggat ctcgggagag gaaatataat taacagaagg
    10621 aaacccagcc agagaattta cttcatttaa taaaaccaag gcaggcaggt gcgtagttga
    10681 tgaacaaacc attttgctga caggaacttg aatccttatt ccgttctgag gaatatcatt
    10741 tttatcatcc ctcgtaagat aatatacttt ttcacttgtg gaacctttaa ataatcttgt
    10801 tatggttact ttcttatagc caggataaga ttcaatgttg aatccccgtg catatttgat
    10861 agtaccatga gatgattcaa taaccttggc ttgctcggcg ttattgcaac cgaggaatgt
    10921 gttcattatg aataacaaaa ttaatcgctt taatccgtcc atattatcat ttattgtact
    10981 gatcaacaat ttctccggcc gcccgtgttg gagtaatgga tttgttcata attttctttt
    11041 ccatttcctt aattatcgaa ggtattcctt ttgatgtttt aaaattttcc aacaacatcc
    11101 attccatggt tgaatgaaac cattgcaaaa gctgtctgtt tcgctggatg ttccagaaat
    11161 cattttccct ggttatattt tcatactcgc aaatagcttc ccagatttcg gataatcctt
    11221 tgctttcgaa ggcggaacac gtaatcacct ttggtttcca gtctgactcg ggcattgaat
    11281 aaaggtgaag tgctgaatgg aattgagccc gcgcctggtt agccgcttcc acattatctc
    11341 catctgcttt ggtgatagct atcaaattag ccatttccat gattcctctt ttcataccct
    11401 gaagttcatc acccgaccgt gccaacataa gtaacaggaa gaaatccacc atttccctta
    11461 cagcaagttc attttgtcca acaccgacag tttcaaccag aataatttca aatccggccg
    11521 cttcacacaa aagtatactt tcccgggttt ttgccgcgac gccaccaaga ttacttcctg
    11581 taggtgaagg tcttatgaat gcagcgggat ttctggccaa ttgctccatg cgggttttat
    11641 cgcccataat actaccacct gaaaatttac tgctgggatc gactgccaga atagcaattt
    11701 ttttccccag gttagtaagg tgtgtcccga aactttcaac aaaagtactt ttaccaactc
    11761 cggggatgcc tgttatccca atccggatgc tatttccggt cctttcaaga attccttcca
    11821 ggacttcacc ggcaatgttt tgatccggag gcaaatgact ttcagtaagg gtaattgcct
    11881 ggctcaaaat agggatgtca cctttcaaga ttccatctat ataatacgcc gatgtttccc
    11941 gatttttcgt tttatggtac attcttgcta atttaaacca tccaaaccat tagaattgat
    12001 tgttttcggc aattataaag acttttaacc caaccacctg atatgatttt taagcagaaa
    12061 tcttactgga tagacgtagt atttggaacg ctatttattt tctccataat gtttgccctt
    12121 ggtagtctcc ttgacaaact tgaatttata gatccaattg gggatgcgct ggaagatatg
    12181 gagatcactg accgggtatt ttctgatccg gacctcagag aaattctgga cgctgatacc
    12241 aatattgtcc tggtcaatat cggattgctt agcaggggag aaataagtga acaaataaat
    12301 atcattaaca aatatcagcc taaggtcatt gggttagatg ccgtcttcac cagtcttaaa
    12361 agtgacaccc tgggtgatct aatgcttgcc aacgcgcttg ccaatacacc taacattatt
    12421 atgtatggga aacttattga acctgataat gatgaggttt ggaatggaat ggaattaagt
    12481 catccgattt ttacccagga ccatgaaatt gcacatgtaa atcttgttac tgaaagcgca
    12541 ggaactgagc aatttcaatt taagacctgc cggagttttt ttcctatgga aaaacttcgc
    12601 gatccggaaa ccaatttgat aacggatgta ccggcattgg cagttaagat ttgtgaaatt
    12661 tataatccgg aagttactgc cggatttctg gaaaggcaga ttgatgaaga gattgttaat
    12721 tacaggggca acataatgga ttttgggctc acgaaatttg gtggccgatt ttatgcattg
    12781 gatgttgatg acgttttgaa tgagaatttt gatccttcat tgttaaagga taaaatcatc
    12841 ttaatgggat ttcttggcca gtatatcgga gaccccaata cagtagagga taaatacttt
    12901 acgcctatga attcaaaatt tgcaggacgg gcacttccag acatgtttgg agtggttatt
    12961 catgccaaca tcgcctctat gattttgaac gaagattata tgaaccaaat gactgaaatg
    13021 gggtcagctt tgtttgctat aattgtctgc atactcaatg tggccgtttt tcaaattatt
    13081 tacataaaaa tggatcggtg gtatgatggg cttacaaaac taattcagct ggttgaagct
    13141 gttttattac tttttgtaat cgtattagtt tttcattatt acagttttaa gctagattta
    13201 acctatggaa tcatagcaat cttgctggct ggggattccc tggaggtgta caatggcgtg
    13261 gttaaaaata tatttaggaa agaaaagaaa aaaaagccgc ttaccattcc ggaagaaaag
    13321 gtatcaattg gcaacacaaa ctaacataaa acttacatta tgagaacttt gctatcaatc
    13381 ggagttgggt tattactgag tactgcaata ttcgcccagg attatgagtt ccgtgttatg
    13441 atgaataagg gagaaaacca gatcaaatca tcgggtggag attgggaggc acttaaaagt
    13501 ggtacccggc taaacaaagg tgatgagctt aaattggcca gcggagctta tctgggtctc
    13561 gtgcataagt ctggcaagac aatggaattg aaagaagcgg gaacccataa gatcgatgat
    13621 ctggcatcaa aaattggtgt cggtaatacc agcattacca gcaaatatgc agattttgtt
    13681 atggcaaaaa tgacggagga ttcaaaggaa gaaaaccggc ggaaatatgc cagtgttacc
    13741 ggagccgtgg aaagagctgc tgatgacgca agcataaaag tgttaatgcc aacgtcagtt
    13801 gatgtgtaca ccaccaatcc cattttggcc tggaatccaa ttgaagaaaa taaatacaaa
    13861 gtggtagtta agaactggtt tgacaaaact cttgacacct atgaaaccac ggaatcctgg
    13921 attcgcttaa atctggaaga tcccaaactg gcaaaagagg caatgcttgt tgtttcagtg
    13981 agcctggcca acaatgaaac tgtaaattca ggtgattatg gcctaaagaa actgacggaa
    14041 gcagatgcat tggatttttc tgaagaactg accgagctga aatctgcact aacggaagag
    14101 tcctccttaa acagggttat tctggcagaa ttctttgagc aaaataattt gcttgtggat
    14161 gcacttacca actatgtaat tgcaattcaa ttatccccga atgttgatta ttttaaagaa
    14221 gcttttgagg aattcaaatt aaggaatggc cttaaatgat aattgatata agactttgaa
    14281 aggggctttt tggccctttt tttttgatta gccgtttatc tcatttttaa gtggggaggc
    14341 cagaccgcca agttgaaatt ccgggaatga attttacttc tgaattttag tattgtgttc
    14401 ggaaacgaac ctttttagat gtaattaacc ttttataaaa cattttggtt caaaagtcaa
    14461 acaaaagggc ctgaaattac gaataagttg gtagcaagaa ctcaaaaaaa taaattccaa
    14521 tgaagaaaat ttatcacatt ctcacaatga tgattgccgt ggcggttttc gcggcatgtt
    14581 catctacagc caacaccggt actacaacag ccaagaagga aaaagattac tctaacctga
    14641 gctctttggc cgatgtacta agaacagttg ggggcattca ggtctcagga gtaggaagcg
    14701 atgttactgt ttccatcagg ggtcaacaaa ccattatgct aaatacccag cccctttacg
    14761 ttattgacgg gttgccaatt ggaaatgact a
//
LOCUS       DASWHX010000164         5778 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_168587, whole genome shotgun sequence.
ACCESSION   DASWHX010000164 DASWHX010000000
VERSION     DASWHX010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 5778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5778
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168587"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(106..1074)
                     /locus_tag="VGA21_11005"
     CDS             complement(106..1074)
                     /locus_tag="VGA21_11005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009579465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HEX9651082.1"
                     /translation="MRAIVYTKYGSPDVLQLEEVEIPSPKNDEVLVKVHAASINSWDW
                     DLLRGKPWIVRLWGLLKPKYNIPGGDIAGTVESVGNNAKNLKPGDEVFGDLSEYGWGG
                     FAEYVSVPEKALTIKPKEMTFGEAAAIPQAGLLAFQGLQDKKQIESGQKVLINGGGGG
                     VGTFAIQIAKSFGAEVTAVDHTAKLDLMRSLGADHIIDYTSENFMGSKKHYDLILDVV
                     ASHSISDCQQVLNPGGFYVIIGGNMTAIFKAMLLGPLITKMSDRGFGIMGYKPNRDLE
                     TMKELYENGKVKPIIDRSYSLNEVPEAFRYFGEGKVKGKIVIAIIG"
     gene            complement(1124..1927)
                     /locus_tag="VGA21_11010"
     CDS             complement(1124..1927)
                     /locus_tag="VGA21_11010"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX9651083.1"
                     /translation="MIEYMEQFKKSFKDYFSKHSDLYLKYRPTYPVELFEYLSTLTQE
                     HDLAWDCGTGNGQAATGLTKFYKNIFATDPSEEQIRNALPHKKIIYRVEKAEHSTLNN
                     NSVDLITAAQALHWFEFDKFYAEAKRVLKENGIIAVWIYKLPLISCEIDKIIKHFHDY
                     TVDEFWQFENRLVEKKYSTLHFPFEELPTPEFSIEKFLTLPELLGLIHSWSAVQRFIQ
                     KTGRDPVLELKDKLMTCWGLPEQKRKIKWKLTLKVGKNNFSLGRILASL"
     gene            complement(1943..3595)
                     /locus_tag="VGA21_11015"
     CDS             complement(1943..3595)
                     /locus_tag="VGA21_11015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013455056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651084.1"
                     /translation="MKKIFTFVSLPFLFFLMGCQASQEQSSASSGDYKDLVAFFKEWR
                     DFEKPPIRDGAPDYTAESFDKRWPEFLSLQARLQAMDTSSWTIGQQVDWFIVWAELNG
                     FSFNYYVLKPWIRDPAFYKSLYTARSDVPAHEGPTHHLTTEIWTYTSPLSSEERNKLL
                     IELRVIPPLNEQAKLNLTGNAKDLWTAGIRDIREQSEDLRDMANLPGVNNDQELLKII
                     QEAITSTDELTAWLETEAKSKTGPSGIGKENYTWYQQHVHLLPLTWEEEVMLLKRELA
                     RAWSALKLEEHRNRNLPELIAADSPEAFMELAEKSAQSLMNFMEQEDIVTVKEYFDPA
                     LREHLGEFTPVEKRNFFSIGAHYDPRPLYSHFYHWFELARMDNEPHKSEIRRGPLLYN
                     IFDNRNEGMATAVEEMFMQAGLYDDNPRVREIVYIMIAQRAARGLGSLYAHANEMTME
                     EAGSIHSEYTPRGWMKTEKELLIFEQHLYLRQPGYGTSYITGKYLIENALAEFARMKE
                     LKHEPFRIKDFFDQLNSIGNIPVSLGHWQMTGNNHQVKELGK"
     gene            complement(3830..4885)
                     /gene="arsB"
                     /locus_tag="VGA21_11020"
     CDS             complement(3830..4885)
                     /gene="arsB"
                     /locus_tag="VGA21_11020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020530413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HEX9651085.1"
                     /translation="MVTKKKLSFLDSYLTFWIFIAMAFGIGMGYLFPSFPKIIDSFTS
                     GTTNIPIAIGLILMMYPPLAKVNYSLLPKVFIDVKILSISLILNWVIGPVVMFILAIT
                     FLREYPEYMVGLILIGLARCIAMVLVWNDLAEGSSEYGAGLVALNSIFQVFAYSFYAW
                     LFITKLPPLFGFEGAIVDISIGTIAESVVIYLGIPFLMGILSRLILVKLNGEEWYSKK
                     FIPTISPITLIALLFTIIMMFSLKGELIVEIPMDVLIIAIPLLIYFAFMFLIGFFISK
                     AMGADYDKNTAITFTAAGNNFELAIAVAIAVFGLNSGQAFAGVIGPLVEVPALILLVR
                     VAFWLRRKYYGNANALP"
BASE COUNT         1696 a         1249 c         1100 g         1733 t
ORIGIN      
        1 agtaatactt gtcaatccct ccccagccgg aaataagttg gttcttcatt gtttgttcct
       61 ggttgtcatt atttcgtcgg ttcaaacaaa aactctttgc ggaaattacc ctattatcgc
      121 aataacaatt ttgcctttca cttttccttc tccaaaatac ctgaatgctt caggaacctc
      181 atttaaggaa taacttcgat caatgattgg cttcacctta ccgttttcgt ataattcttt
      241 cattgtctct agatccctgt tgggtttata ccccataatt ccaaaacccc tatcgctcat
      301 ttttgtaatc aaaggtccca gcaacatggc tttgaaaatc gcggtcatat tgcctccgat
      361 tataacataa aaacctcccg gattcaaaac ctgttggcaa tccgaaatag aatgagatgc
      421 cactacatcc aggatcaggt cataatgctt tttactcccc atgaaatttt cgctggtata
      481 atcaatgata tggtctgcgc cgagcgaccg catcaaatct aacttcgcgg tgtggtctac
      541 cgcggtgact tcagctccaa atgatttggc aatctgaatc gcaaaagtac cgactccacc
      601 accaccgcca ttaatcaaaa ctttttgtcc ggattcgatc tgttttttat cctgaagacc
      661 ttgaaacgcg aggaggcctg cctgcggtat tgctgccgct tctccgaagg tcatttcttt
      721 gggtttaatt gttaacgctt tttccggcac tgaaacgtac tccgcaaaac ctccccagcc
      781 gtattcactg agatcgccaa acacttcatc tccgggtttt agattctttg cattattgcc
      841 aacagattca acagtacccg ctatatcgcc gccaggaata ttatacttcg gtttcagaag
      901 tccccaaagc cgaacaatcc agggttttcc tctcagaaga tcccaatccc aggaatttat
      961 cgatgccgcg tgaaccttca ccagcacctc atcattttta ggagaaggta tttctacctc
     1021 ttcaagttga agaacatccg gtgagccgta tttggtataa acaattgctc tcatttaatt
     1081 tccattttta attaaattta tcaaattgat ttattcacgt gtatcagaga gatgctaaaa
     1141 tcctccctaa ggaaaagtta tttttcccca ctttcaaagt aagtttccat ttaatctttc
     1201 gcttctgttc aggcagaccc cagcacgtca ttaatttatc cttcaattcc aaaacaggat
     1261 ctcttcccgt tttttgaata aaccgctgaa ccgccgacca gctatgaatt agtcccagca
     1321 gctcaggcag ggtaaggaat ttctcaatac taaattccgg ggttggcaac tcctcaaacg
     1381 gaaaatgcag ggttgaatat tttttttcaa ccaaccggtt ttcaaactgc caaaattcat
     1441 ctacggtata gtcgtgaaag tgtttgataa ttttatcgat ttcacaggaa atgagtggca
     1501 gtttgtaaat ccagacggca atgattccat tctctttcag cacacgcttt gcttccgcat
     1561 agaatttatc aaattcaaac caatgtaagg cctgtgctgc tgtaattaaa tcaacagaat
     1621 tattgttcag tgttgagtgt tctgcttttt caactctgta aataattttc ttgtgaggca
     1681 aagcatttct aatctgttcc tcacttgggt cagttgcaaa aatattctta tagaatttgg
     1741 taagacccgt tgctgcctgc ccattaccag ttccacaatc ccaggcaagg tcatgttcct
     1801 gggtaagtgt tgagagatat tcaaataact caacaggata tgtgggcctg tatttgaggt
     1861 aaagatccga atgctttgag aaataatcct tgaaagattt tttgaattgc tccatatatt
     1921 caatcattaa aatcctggtt ttctatttcc ccaactcttt tacctggtga ttatttccag
     1981 tcatctgcca gtggccaagg gaaaccggaa tattgccaat ggaattcaac tggtcaaaaa
     2041 agtcttttat ccggaatggc tcatgcttca attccttcat ccgggcaaat tccgccagtg
     2101 cattttctat gagatatttt cccgttatgt agctggttcc ataacccggc tggcggaggt
     2161 agaggtgttg ctcaaaaatc agcaattcct tttcggtttt catccaacca cggggagtat
     2221 attccgaatg gatgctacct gcttcttcca tagtcatttc attggcatgc gcatagagag
     2281 agcccagacc tctggctgcg cgctgggcaa tcatgatgta gacaatttct cttacccgtg
     2341 gattgtcatc gtataatccg gcctgcataa acatttcttc caccgctgtt gccatacctt
     2401 cattgcgatt gtcgaaaatg ttgtataaca gcggacccct acgaatttca ctcttatgtg
     2461 gttcattgtc catccgggcc agttcaaacc aatgataaaa atgcgagtat agcggcctgg
     2521 gatcataatg agctcctatc gagaaaaaat tccgtttttc cacaggagta aattcaccta
     2581 aatgctcccg caaggccggg tcaaaatact cttttactgt tactatgtct tcctgttcca
     2641 taaaattcat taagctttgg gcggatttct cagcaagttc catgaatgct tcaggcgaat
     2701 cggcagctat gagttccggc aaattgcggt ttcgatgttc ttccagtttc agtgccgacc
     2761 aggcccgggc caattcgcgt ttcaggagca taacttcttc ttcccaggtc agcgggagca
     2821 ggtgaacatg ctgttgatac catgtgtaat tctctttgcc gattcctgaa ggaccggtct
     2881 tcgatttagc ttccgtctcc agccatgcag ttaattcatc cgtggaagtg attgcctcct
     2941 ggatgatttt aagcaattcc tggtcgttat tcacaccggg tagatttgcc atgtctctaa
     3001 gatcttcact ttgttcccgg atatctctaa tccccgctgt ccaaagatct tttgcattac
     3061 ccgtcaggtt aagctttgcc tgttcattca gcggaggaat tacacgaagc tcgatcagca
     3121 acttgtttct ttcttcagaa cttagtgggg aagtgtatgt ccagatttcg gttgtcagat
     3181 gatgggtggg accttcatga gccggcacgt cgctgcgtgc agtataaagg gatttataaa
     3241 atgcagggtc acgaatccag ggtttcagaa catagtaatt aaaactgaat ccattcaatt
     3301 ccgcccaaac aataaaccag tctacctgct gcccgattgt ccacgatgaa gtatccatgg
     3361 cctggagtct ggcctgtaaa cttaaaaact ccggccagcg cttgtcaaag ctttcagcag
     3421 tgtagtccgg cgcgccatcg cgaattggag gtttttcaaa atcccgccat tctttaaaaa
     3481 aggcaaccag gtccttgtaa tctccggaac tcgcggaaga ttgctcttga gaagcctggc
     3541 aacccattaa aaaaaacaag aatggaagtg aaacaaaggt gaaaatcttt ttcatagttg
     3601 aggttattaa tgcaggttgc actgcatgaa agtatttaaa tacaaactaa tttaattatt
     3661 tatcagaaga aaatgtcaaa gggaaaacaa tccttccggc gctaaccaaa cactaactgt
     3721 tttctgccac ctgttctaat ttatttggga tgcgagcgcg ttgtgaatta gcaaaggcaa
     3781 aagtctttat aaatgttcag gttaattagg atgtaccgag taaatttagt tatggcaaag
     3841 catttgcatt tccgtaatat tttcttctta accagaatgc gactctaacc aatagaatca
     3901 atgctggaac ttccaccagt ggcccgatta cgccggcaaa tgcttgtccg gagttaagtc
     3961 cgaaaaccgc gatcgcaacg gcgatggcta attcaaaatt atttcccgcc gcggtaaagg
     4021 taatggccgt attcttatca taatccgcgc ccattgcttt gctaataaaa aatccaatta
     4081 agaacataaa agcaaagtaa atcaatagtg gaatggcaat gattaaaacg tccataggaa
     4141 tttcaacaat cagttcaccc ttaagagaga acatcataat gatggtaaac aaaagcgcga
     4201 ttaatgtgat tggcgaaatt gtgggaataa attttttgct ataccactct tcgccattaa
     4261 gtttaactaa aatcaaccgg cttaagatac ccatcagaaa tggaatacct aagtaaatca
     4321 caacactttc cgcgatggtt ccaattgata tatcaacaat agctccttca aatccgaaaa
     4381 gcggcgggag ttttgtgata aataaccagg cgtaaaaact gtatgcaaat acctgaaaga
     4441 tgctatttaa ggcaactaaa cccgcgccat attcgctgct tccttcggca agatcattcc
     4501 aaaccaaaac catagcaatg cagcgagcca ggccaattaa tatcaagccg accatatatt
     4561 caggatattc gcgcaaaaaa gtaatggcca atataaacat taccaccggg ccgattaccc
     4621 aattcagaat gagtgagatg gatagaattt taacatctat aaaaactttt gggagcaaag
     4681 agtaattaac ttttgccaat ggaggataca tcattaaaat caatcctatg gcaataggaa
     4741 tatttgttgt tccgctggtg aacgaatcaa tgatttttgg aaatgatgga aataaatatc
     4801 ccattccaat tccaaatgcc atcgcgatga aaatccaaaa ggttaaataa ctatcaagaa
     4861 aacttaattt tttctttgtt accataatta cattaaattt tcaagataca atgatagcca
     4921 tgtgtttatg gcagtgccaa atgagttgta agattaagga aaaaactaat attttcccgg
     4981 ctaatcactt caataatacc gtgtatgagg atttggcatt atttgaaata catccaaatc
     5041 aaatttgaaa accgttcgga tgatttattc catttattgt atgaaattgt tccttgttcg
     5101 attaaaatca tttcaccatt acaatttacc gaacacaaaa cattctcagt catttgtgca
     5161 aattgtaata aaataagcac ccgcgcgcga ataatcatat ccttttaatc ggatagaacg
     5221 tctgtgatgg acttgtgggt tgtagctcat tattcaatgt gcaatcacta actgttcaat
     5281 gatattttcg gtgatggttt catttaccta aaaaatttat catctgccca ttttgcagga
     5341 ttgtttatga tataatctga aatggtttga tacgattaat aattaatctg aagattactc
     5401 attaactcgg gaaagaagca gctttagttg gtgaaagaat atcagcgacg cttatttttt
     5461 aattactgat tggatacact ttattaagtg cgttataaac gcacctttca atggcacctc
     5521 gttacaaacg agggccatcg ttgggggcca tttagtcatt tgtcaccgga aattcttcga
     5581 agtcaaggag ggtgttcaac gaactgtgtc aagataaaaa attattaact tgatacgaat
     5641 tatgaaagaa aacatgaaca tggaagagtt tcgcaaacaa gcagtggaac ttcttcgaca
     5701 aggaaaaccg ttaacgggaa aagatggggt aatgacgccc ctgataaagc aggttctgga
     5761 agcttcgttg gagggtga
//
LOCUS       DASWHX010000165        24797 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_2041783, whole genome shotgun sequence.
ACCESSION   DASWHX010000165 DASWHX010000000
VERSION     DASWHX010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 24797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24797
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2041783"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2092
                     /locus_tag="VGA21_11025"
     CDS             <1..2092
                     /locus_tag="VGA21_11025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020530145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX9651086.1"
                     /translation="EINLITQLSMDTELLIKHDVKSFNEQGIYADKTLFSVFSFSKIK
                     GNPDNPLPDIKSIAISQKLAQKLFGDDNAIGRSLQVGQTYDLIVTCVFDDIPENSSLQ
                     FDFVISFDLFVKENPWTQNWRSGGTRTLVTLKPGASIMSANEKLEELIKKNCDDCTTN
                     PFLFPYTKSRLYNNFENGKSAGGRIEQVVLFSLVAVIILTMACINFMNLATARSASRS
                     REVGVRKIIGAKQSSLIIQFIIESVFMSFIALLFALGVVQVFLPLFNTIADKSIQLDF
                     TNQFITGTLIITLISGLLAGSYPAFFLSSFKPIVALKGQVQSSFSGNGLRKTLVVIQF
                     MTSIVLIIGSIIIYNQIRFISIKNLGFDKESVIVIDKNEDILKNYSAFKNDLLQFPSI
                     TSIGFGGSNIFTIPITSTDPVWPGKLENSSITFKIFRCDEGFIPTMGIELIAGRNFIG
                     LNPQDASNYIINKKAMEVMGLTPENVIGADLEMWNGKGKIIGLTNDFHNDNLRVGIEP
                     LILLYSVNNGWHYFVKVDEKTPISASLATIEQVFKNYSPDHPFQYSFLDEIFDREYRT
                     EAIIGKLSLSFTVVAVLISCLGLFGLASYTAERRTKELGIRKVMGASIRNLIILLCRD
                     FSKLVVISLFLGCPVAWYLMREFLAGYAFHTELGGSVFIITSVALWLIALLTVGYQSV
                     KAALANPVESLRNE"
     gene            2331..2741
                     /locus_tag="VGA21_11030"
     CDS             2331..2741
                     /locus_tag="VGA21_11030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651087.1"
                     /translation="MTFKGIIQVMSGEIIRNFLVILLLFLYTLGNTEFETLHLVIHPD
                     DSLAHTANQEKDSCHRAIYHNEPEEGCSHKSHVLLKVKCGLCDQVCHSDQVHFTIIQS
                     APLEFAELSQKYNQSFFLTSVQANLIPRAPPALS"
     gene            2823..5240
                     /locus_tag="VGA21_11035"
     CDS             2823..5240
                     /locus_tag="VGA21_11035"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX9651088.1"
                     /translation="MSLKTMFLLSTSLFCMQVIAQDTCTGVLTGKVLDDSQQPLIGAT
                     ILLLPLQKGEISDALGSFSFTGLCPGTYLMKVQYLGYEHAEFQLEINGKVTFDIHLKE
                     ISAELEGVVILDHHDALHTENAINFIELDERKLSELAGKSLGETLKEVTGVNSIQTGP
                     GIFKPVIHGVHSDRVLILNHGLRQEGQQWGAEHAPEIDPFIASNIVVIKDASAIKYGT
                     NALGGVIVINPSELPNRPLLSGTVHTILQSNGRSGAFSSMLEGGIKNMDGWGWRVQGT
                     ARRSGDYNTPDYVLTNTGVKELNFSAATGYHKEKIGFDVFFSHFQTQIGILKGTAIGS
                     PEDLATAMESPIPQYTTDFSYKIGEPRQEVSHNLLKINGHIQTTHGDWRIQYGFQNNN
                     RKEYDIRIGQLSQIPSINLKLNSHSLDLEWETLHSERGTVILGLNNILQNNYNISGTQ
                     RIPFIPNFTNLSTGVFGVAKLLLKTWTMDFGLRYDYRYYDVTGYDFKNSLYSTTYNFH
                     NISATLGTKVSLAKDHVILLNLSSAWRPPHVAELLSIGRHQSAAAIEYGLLLQDTTNE
                     VRSIGDVNFNTEQSVKFVATYQWNLQSFSLEASPYANYIFNYIFLRPYGVTKSISGTY
                     PFFRYTQTDALFLGIDLSGSWRPVANFQVIPKASLLRASDEQNNNPLVFIPSNKYDLT
                     FRYEKPAFLFIKSFYIESKSIYVARQTRAPRVISPREIVEAQVEGVDLFKDNTSIFDF
                     QAAPKGYFLWNISIGASIRRSHVQYNLRISSENTLNQKYREYTNRLRYYADDIGRNIT
                     LSLKCIF"
     gene            5262..5906
                     /locus_tag="VGA21_11040"
     CDS             5262..5906
                     /locus_tag="VGA21_11040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651089.1"
                     /translation="MKNKKRIKHNLNLLSVSLMGVCLLIFLLSGCKDNDLVKEDVPEL
                     ITKASLTFTPSGAGNVIVVTATDPDGEGVQDISVDGPINLNADETYILTLSLINELAD
                     ATDPEYDITDEVEAEGDEHMFFFSWTNNVFADPSGDGNIDNRSDPVNYTGASNSVDVN
                     NRPLGLTTTWITATGSSTGTFRILLKHQPELKTDTSNSIIGETDLDITFTINIL"
     gene            complement(6266..6904)
                     /locus_tag="VGA21_11045"
     CDS             complement(6266..6904)
                     /locus_tag="VGA21_11045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017733072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEX9651090.1"
                     /translation="MAVLRYLLVISLMVIAITGLAQDKPERKIVQLSGIVIGEDSTAT
                     LPGVHIYVPTAGRGTTTNAYGYFSLPVLTGDSLIISAVGFHKQNFIVPFNQGDDITEL
                     FQLEVDTIFLENVDILPYPTEEALKQAWLALRLPDETKQLRESLNGEYLAYMIKNTPL
                     DGSLSASYYLDQQLYYQINRYGGISNPFLNPFNWVRFIQDLKSGKYKKKDKN"
     gene            7032..7418
                     /locus_tag="VGA21_11050"
     CDS             7032..7418
                     /locus_tag="VGA21_11050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008202822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX9651091.1"
                     /translation="MRLKNLSLPFSAQVFKSFSEEPRVRIINLLFHKEELCISDIEQI
                     LEFTQTKTSRHLIYLKNAGIVSYRKVDQWVFYYIKEEVMDLVSRILDFLQKDQVLKAD
                     LTTYETLFSNRELSINRIKFSKLNKA"
     gene            7419..8114
                     /locus_tag="VGA21_11055"
     CDS             7419..8114
                     /locus_tag="VGA21_11055"
                     /inference="COORDINATES: protein motif:HMM:TIGR02254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HEX9651092.1"
                     /translation="MSNYQHLFFDLDHTLWDYEANAAEVLDDLYHRHTLGKIINSGSR
                     HFTEVFFEINNRLWDQYDRGLIHKSVIRNERFDRILAAFGVSNSRLAKTINLAFISEC
                     PYKTNVIPGAVELLSHLRPKFGIHIITNGFREIQWIKIAKSGLDKFIDQVFISEEIGA
                     RKPDPKFFEFSLNKVECTRAQSVVIGDNLNTDIKGARDYGIDHIFYNPKKLTHTDRVT
                     WEINHLSQIPELI"
     gene            8173..9804
                     /gene="pruA"
                     /locus_tag="VGA21_11060"
     CDS             8173..9804
                     /gene="pruA"
                     /locus_tag="VGA21_11060"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008627247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HEX9651093.1"
                     /translation="MSTGFYTIPEPKNEPIFDYAPGSMQRGELKKAIEKLRSGKADIP
                     MYIGGEEVRTNNKIRISPPHDHKHTLGFFHVGDKNHVEQAVNAALNAKSSWTSMSWEQ
                     RAAIFLRAADLVSGPYRYILNAATMLGQSKNPFQSEIDAVAEYADFLRFNAHYMEQIY
                     KVQPISPALTWNRLEYRPLEGFVFAITPFNFTSIAGNLPASAALMGNTVVWKPAYTQV
                     YSANILMKIFIEAGLPRGVINLIYVDGPVAGDVIFEHPDFAGIHFTGSTSVFRKLWET
                     IGKNIAKYKSYPRIVGETGGKNFIIAHPSANAAQLSTAIVRGAFEYQGQKCSAASRAY
                     IPSSLWPAIKENVSKALKEIKMGGCEDFRNFCNAVIDEKAFDKISAYIEKATSSNVAE
                     IIAGGKCDKSKGYFIEPTVILAHDPQFATMCEEIFGPVITIYVYNDDNWEETLDLVDQ
                     TSPYALTGAVFSIDRQAIQLATKKLVNSAGNFYINDKPTGAVVGQQPFGGARGSGTND
                     KAGSLFNLLRWVSVRTIKENFLPPSDYRYPFMKEE"
     gene            10059..10652
                     /locus_tag="VGA21_11065"
     CDS             10059..10652
                     /locus_tag="VGA21_11065"
                     /inference="COORDINATES: protein motif:HMM:NF019350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX9651094.1"
                     /translation="MARDFHDEMGNHMASIISMVNILSMKLKTNDQQILSILKKVEFA
                     SKELFQGTREFMWTIDPDHDNLVEILIYLRDYGVDFFQNTNIDFEVGKNLLTGAEALP
                     VQPGSCKHILAIFKEAMTNCLKHSHAAKVTLSSESTNHSFTIIFCDNGIGVREKMGRG
                     LQNMQTRALKVNAILKCENMEQGFSVALKFNNLKIVI"
     gene            10674..11330
                     /locus_tag="VGA21_11070"
     CDS             10674..11330
                     /locus_tag="VGA21_11070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020531666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX9651095.1"
                     /translation="MRESRFNYNTLDRRTRIVIIEDNEQIREGYALILNTNSNYNVVS
                     TYNKCEKALKYLHKDNPAIVFMDIELPGMDGIRGIKEIKKLRPDIDIVVISVYDENDK
                     IFDALCAGASGYITKSSNHMELLHAVDQVLKNGAPMSSNIARKVVQSFQRNYNTPLSA
                     RETQVLSFLASGATYQSISRDLEIGLETVKTHVKHIYTKLQVSSKTEALELAKKGNYI
                     "
     gene            11519..12082
                     /locus_tag="VGA21_11075"
     CDS             11519..12082
                     /locus_tag="VGA21_11075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651096.1"
                     /translation="MKNNFRLLNHPFYQAWSRGKLTPNQISIYGSSYLELIEQIPTYW
                     QKILHSFGIEDTLDAQQIVKEEKEHINLWKKWIIKLQEPKDFPSMKDVTASLFKISES
                     ELLGAISSFEMQQPKVANTKKSGLLEHFGFEAEDLTYFDEHLKEEKHIQFAKRVREHY
                     SNHSEFQQGYNFGKKLFYEALDRFLSN"
     gene            12547..15768
                     /locus_tag="VGA21_11080"
     CDS             12547..15768
                     /locus_tag="VGA21_11080"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HEX9651097.1"
                     /translation="MKKNLLYFLWVLCLVMIQNKASAQERVVSGKVTSSEDGSPIPGV
                     NVILSGTTTGTITDAEGTYRMSVPQEGGTIIFSFVGMKSIEVAMGSQSVIDVQMESDF
                     QELGEVVVTALGSERNASSIVYANQTVKGEDLLSTPNKNALEALRGKAAGVRLSTGSG
                     SVGASTRIVLRGEGSLTGNNNALIIVDGIAIDNTSTSGGAGTSTGGYADHGNRFNDIN
                     PDDIESVTILKGPSATSLYGSRGASGVVLITTKKGKKGDVQVRLNSSYSQEKAYILMQ
                     RQDRFGQGYDNAHFDSGENWSWGPEFDGVVRPWTSPIDADGDGSLESLIRPYSAVPDQ
                     IQEFFNTGNTLSNSISFSGANEGFTYYASFSNVGQSGILDNTKYKRNTVNFNATAKLS
                     EKLSSDFKISYASINQNTAQEGSRAFEGNNAYAMVLQSPVNIPFGELRDYNSPFHGID
                     GYWGSYSSVNPYFILNEFGNEGKINNVLGNASLSYNIIEGLDFTTRFGSNVVSTSVET
                     WTPKFTPSTQLIWGDDLELATRNSKHASPGDYTYFHGLTINRDVTATLNYMKNLTDDI
                     SMDISAGYNFFQRERKNSTGTTNGGLLAEGIYNLGNSVGNPIASLERRKYRIYGVLAN
                     ARFGFRDALFLEYSVRNDWSSTLPENSNSFLYQSVGTSVVLNDLIGMDDNILNFAKIR
                     GSIGTSGKDAGLYLLNSSFTSNPEVFSGDNPVRFPINQQPGSTVGNLIGSPNLKPELT
                     LTYEFGSDLGFFGERIEISYTYYKSEHRDQIVEVSLPRSSGFTTTASNIGLMTNKGHE
                     LTLSVKPIYGVVDGLDVELFGSWAKNVNEVVKVADDISELSFGTYTPGVTVVAKEGEP
                     FGTYKSLAPLTNQDGQVIVDPNTGYPLYTEQEVYLGSYQPDFIASFGMNAKFKGIGFN
                     ILFDMKQGGNFGSQTKFFTEFNGTSEHTAIYNRESFIYPNSVIENDDGTYSENTIEIT
                     EQDLFTNYDPAASTYIIDASFLKLREIGLSYDLPRNLMGKIPFSSARISLFAKNLKFW
                     LPGENTFADPEVNGPALTGNAQGIETSQTPSSKSFGVNLQLTF"
     gene            15791..17230
                     /locus_tag="VGA21_11085"
     CDS             15791..17230
                     /locus_tag="VGA21_11085"
                     /inference="COORDINATES: protein motif:HMM:NF024181.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HEX9651098.1"
                     /translation="MKNIFKSSIAFTLILFMSCEEKITELSNDPNVSAIANDSEVLTS
                     AIGYMGAIQDVDLNQGSFLWAQYYTWGIGVSIGNQERYVAQPDDRNGYWQRAYANCLA
                     DLNYLRSSESAAYRGVAGILTAYVFQGLVDHFGDVPYSEALQGENGVLTPAFDSAPTI
                     YVSLVSSIDSAMDELALATNDIGDDDLVYGGDLNQWVKFANSLKLRLLMRTSEVDAKG
                     SEITTLVSSGTFLESSADIAQISWSGTTGNSNPMWSVQMNGVGDFYFASNAMVNLLNS
                     LNDPRLTTFYAAATTGAFNGQIRGINQGTIDDEPFTAPATDYSGSSAYANGATAPTVL
                     MSPWEVWFLRTEAAARYGTADNEATAFANAVQANFDYMGIAGGAAYITSLSYSTTDPL
                     NDRLNTIGIQKWISMNGTQEDEGWIETRRFDRPGDRIFTSGIFQNPPLSVLAAGTFPA
                     SWLYPATERSYNPKAPGQRAITDQLFWDN"
     gene            17243..17983
                     /locus_tag="VGA21_11090"
     CDS             17243..17983
                     /locus_tag="VGA21_11090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651099.1"
                     /translation="MKYYILLIIALVAFSSCKEYENSAEPSSVTYLPLIEIQGEPEID
                     LECNVGGYTDEGAVATEGGTSIELTTSVIGVYFEGTAVNIPDLYHITYSAENVDGIPG
                     AETRSVTWPACNSDLVTGIEGMYTAAVFRTATSGATVDYSDAEHGPYWVVDLGGGQYG
                     FSCVIGQFYEYGSDYGYGPAYAGTGFVVTANDISTNDFTITATNGVGAFGRDIEVTDF
                     TVDSSAGTISWTATWSAGYEFDVVLTQN"
     gene            complement(19215..20081)
                     /locus_tag="VGA21_11095"
     CDS             complement(19215..20081)
                     /locus_tag="VGA21_11095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014796981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HEX9651100.1"
                     /translation="MRQLKITQNITNRENQSLEKYFQEVNKVDLLTIEEEVTFAKRIQ
                     EGDEAALEKLVKANLRFVVSVAKQYQNRNLTLNDLINEGNLGLIKAAQKFDHTRGFKF
                     ISYAVWWIRQSIMQALAEQSRLVRLPLNKVGTISKISQAASVLEQRLERMPTEEELAN
                     LLDMAEDDIKNTMRITTRQLSVDTPFEEGEDSSLLDVLVDDSMERTDEKITYKDSLSI
                     ETERALSTLTERESQIIKMFFGIGEENPMSLEQISEILGLTRERVRQIKEKALRRLRH
                     SSRSKLLAEYLG"
     gene            20333..20611
                     /locus_tag="VGA21_11100"
     CDS             20333..20611
                     /locus_tag="VGA21_11100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="OadG family protein"
                     /protein_id="HEX9651101.1"
                     /translation="MPSSLNTAITLMVIGMTTVFFILALVVGSGKTLILFVNRFFPAE
                     KVSPIPVFIEKSTISKTKMAVIIAVVEKITKGEGQILDIKKLNNGPKN"
     gene            20595..22457
                     /locus_tag="VGA21_11105"
     CDS             20595..22457
                     /locus_tag="VGA21_11105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020532772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEX9651102.1"
                     /translation="MGRKIRYCLVYRDMWQSSGKYMPRADQLVRVAGPIIDMGCFARV
                     ETNGGGFEQINLLFGENPNTALRQWTKSFNEVGIQTQMLERGLNGIRMYPVPADVRRM
                     MFHLKKKQGTDIARSFDGLNDVQNLELSVRYALEGGMIPQGALCITHSKVHTIEYYLK
                     MADDLIEMGATEIGIKDMAGVGRPVSLGRIVKGIKDRHPKILVQFHCHSGPGFSVAST
                     LQAVDAGADIVDVAMEPVSWGTGHADLLTIHAMLEDAGYEVPKINMNAYMKVRSLTQE
                     FIDDFLGYYMSPKNRLMNSLLIGSGLPGGMMGSLMADLELNLESVNKYLTKSGELQLT
                     QDDLLIKLFDEVEYIWPKLGYPPLVTPYSQYVKNVALMNVIQLIKGRERWSMVSDNTW
                     DMILGRNGKLPGPVDPALVELATSQGKEVFNGVPQDLYPDNLDLFRAEMKQNSWDLGQ
                     DEEELFELAMHPEQYRAYKSGKAKEAFHQDLKEKKQEKLVPVTKGEVAVKTDLPRTLD
                     IEVNGEKFQVKIGYPNVGATPISSENGSAGHADLQNLREITAPIEGTFMLTKDSAERP
                     IKVGDIIKKGDLIGYIESMKVYNAITADKNGEVVEICSRNGDQVIEDEVLIRIA"
     gene            22461..23657
                     /locus_tag="VGA21_11110"
     CDS             22461..23657
                     /locus_tag="VGA21_11110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020532771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HEX9651103.1"
                     /translation="MEILRRIYEMTAISEVIKHPAVILMWAIGFFLLYLGIRKKYEPL
                     LLVPIGFGLILANALGGEMNIVQDDHLAELPLVKIAKDHGIMNFLYYSLIKTGFLPPI
                     IFMGVGALTDFGPMLRNLRLAFFGAAAQIGIFTVLFAAIIFGFTPKEAASLGIIGGAD
                     GPTAIYTTIILAPHLLGPIAIAAYSYMALVPVIIPFVVKYTTTQKELLINMKEQDLLY
                     PEKHPIKNIQLMKILFPLFCGGLVAVLVPTAVPLLGMFFFGNLIKEIGPTTERLSQAA
                     SGPIMNTSTIFLGLCVGATMKYETFLDTKTLLIIAGGLIAFCISIAGGITAVKIYNVF
                     SKKKINPLIGATGLSAVPMAARVANDIALKYDKQNHLLHYAMASNISGVIGSAVAAGV
                     LITFLR"
     gene            24120..24193
                     /locus_tag="VGA21_11115"
     tRNA            24120..24193
                     /locus_tag="VGA21_11115"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:24154..24156,aa:Asn,seq:gtt)
     gene            24369..>24797
                     /locus_tag="VGA21_11120"
     CDS             24369..>24797
                     /locus_tag="VGA21_11120"
                     /inference="COORDINATES: protein motif:HMM:NF036423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
                     /protein_id="HEX9651104.1"
                     /translation="MSAYVKLYCKKSRLKKNGEAPIYYVIKLNAKEKLLFSGKTIKPS
                     LFDNESGEIKNKVLYGKLNSCLTTEKVRFNNIILDLEYKKETITFEKILSCFKSPTEN
                     KCFIEFFSNELDAIKNGIAKKTYKDYLVSLNNLKEYRQSLS"
BASE COUNT         7601 a         4716 c         5053 g         7427 t
ORIGIN      
        1 agagattaat ctcattacgc aactcagtat ggatactgaa ttattaatta aacacgatgt
       61 caaatctttt aatgagcagg gtatttatgc tgacaaaacc ttgttttcag tatttagctt
      121 ttcaaaaatt aaaggcaatc ccgataaccc gttgccagat attaaatcaa ttgccatttc
      181 gcaaaaactg gcacagaaac tttttggaga tgataacgca atagggagat cattacaagt
      241 tggccaaaca tatgatctca tcgtaacctg cgtgtttgat gatattcccg aaaactcaag
      301 ccttcagttt gatttcgtta tctcgtttga tctttttgtc aaagaaaatc cttggacaca
      361 aaactggaga agcggtggaa cgcgaactct cgttactctg aaaccaggag cttctataat
      421 gagcgccaat gaaaagctgg aagaacttat taaaaagaat tgtgatgact gcaccactaa
      481 tcccttttta ttcccctata caaagtcacg gttgtataac aattttgaaa atgggaaaag
      541 tgccggcgga cgaattgaac aggtggtact ctttagcctt gtcgctgtaa tcattctcac
      601 aatggcgtgc ataaatttca tgaatcttgc tacagccaga tcagcttcac gcagccgtga
      661 agtaggtgta aggaaaataa tcggagctaa acagtccagc ttgattatac aatttatcat
      721 cgaatctgta ttcatgtcat ttattgcttt gctgtttgct ttaggagttg tgcaggtttt
      781 cttgccactc ttcaatacga tagctgataa atctattcaa cttgatttta ccaatcaatt
      841 cattaccggt actctgatta taacattgat atccggactg cttgcaggaa gttatcccgc
      901 tttttttcta tcttccttta agccaattgt agctttgaaa ggccaggtcc agtcgtcatt
      961 ctcgggaaac ggacttcgca aaacactggt agttattcaa tttatgactt ccatagttct
     1021 gattatcgga agcataatta tttataacca gataagattt atcagcatca aaaatcttgg
     1081 cttcgataaa gaaagtgtaa ttgtaattga caagaatgag gatattctca aaaactattc
     1141 agcattcaaa aacgatttgc ttcaattccc atccattaca agtattggtt ttggaggaag
     1201 caatattttt actattccaa tcacctccac tgatccggta tggccgggca agcttgaaaa
     1261 ttcttccatt acctttaaaa tctttcgttg cgatgaagga tttattccaa ccatgggcat
     1321 tgaactgatt gcaggccgaa atttcattgg cctgaatccg caggacgctt ccaattacat
     1381 catcaataaa aaagcgatgg aagttatggg gctgactccg gaaaatgtga ttggtgctga
     1441 cctcgaaatg tggaatggaa aaggaaaaat tatcggactt accaatgact ttcataatga
     1501 caatctccgc gttggtatcg aaccactgat tcttctttac tctgtaaaca atggttggca
     1561 ttactttgtt aaagttgatg aaaaaacacc aataagcgca tcacttgcaa ctatagagca
     1621 agtatttaaa aattacagtc cggaccatcc atttcaatac tcctttcttg atgaaatatt
     1681 tgacagagag tatcgtacgg aagccattat tggtaaacta tctttaagtt ttacagtggt
     1741 ggcggtgctt atttcttgcc tgggattatt cggtctcgca tcttacaccg ccgagagacg
     1801 aacaaaagag ttgggcatcc gcaaagtgat gggtgcttca atacgtaatc tgatcatact
     1861 gctttgcaga gatttttcca aactggtagt cattagcttg ttcctgggct gccctgtcgc
     1921 ctggtatttg atgagagaat ttcttgccgg atatgcattt cacactgaat taggtggaag
     1981 cgtttttatt attacctccg ttgcattatg gctaatcgcg ttactaacgg ttgggtatca
     2041 atctgtaaaa gctgctcttg ccaacccggt ggagtcatta cgaaatgagt gataaaaccc
     2101 cgaacttgtc gcatggattt gttacggaat gatctttttt gacaaatgcc agtcaaacta
     2161 ctgcactatt taatttatta aatttttacc gaatatcgtg gaaaaagttg cccctaccgt
     2221 ttatggaatt aaagctgatt atccacctga ataaggtttg aattggcatt cgaattgggt
     2281 acttttaatt tgtcatttat ctacaaacat agggttgaga ccaaatctga atgactttca
     2341 aaggaatcat acaagtgatg tcaggagaga ttattaggaa tttcttagtt atcctgctcc
     2401 tctttttata tactttgggc aatacagagt ttgaaactct gcatcttgtt attcatcccg
     2461 acgactccct ggcccatacc gcgaaccagg aaaaagactc ctgccacaga gcaatctacc
     2521 ataatgagcc tgaagaaggc tgcagccaca aatcgcatgt tttgctaaaa gtgaagtgcg
     2581 gtctttgtga tcaggtttgc cattccgatc aagtacactt tactattatt caatcagcgc
     2641 cgttggaatt tgctgagttg tcgcaaaaat ataatcaatc cttttttctt acttcggtcc
     2701 aggccaattt aattcccagg gctccgcctg cactttcttg atttctccgg ggaacaatta
     2761 aattattcct ctaaataatt ccggctcaat tgcccgagat cttattattt aattctgtta
     2821 tcatgagttt gaaaacaatg tttctgttgt caacttcact tttctgtatg caggtaattg
     2881 cacaggatac ctgtaccggt gtcctaaccg gaaaagtgct ggatgattca cagcagcctc
     2941 ttattggtgc cactattttg ctgctacctt tacagaaagg tgaaatatct gatgctttag
     3001 gtagcttctc tttcacaggt ttatgtccgg gaacatacct gatgaaagtt caatatctgg
     3061 gatatgaaca tgccgaattt cagctggaga ttaatggaaa ggtaacgttt gacattcatc
     3121 tcaaagaaat ttccgcggaa ctcgaaggtg tggttattct tgatcatcat gacgcgttgc
     3181 acaccgaaaa tgcaataaac tttattgagc ttgatgaaag aaagctttca gaattagcag
     3241 gaaaaagtct gggcgaaact ttaaaagaag ttactggagt aaacagcatc cagaccggtc
     3301 ctggaatttt taaaccggtt attcatggtg tacacagcga cagagtactt atactaaacc
     3361 acggcttacg gcaggagggc cagcaatggg gtgctgaaca tgctccggaa atcgaccctt
     3421 ttattgcttc caacattgtt gtgattaaag atgcttctgc cattaagtat ggtactaatg
     3481 cattaggcgg cgttattgta attaatcctt cggaattgcc aaaccggccg ctgctgtccg
     3541 gaacagttca taccatactt caaagtaacg gaagatccgg cgctttttct tcaatgctgg
     3601 aaggtggtat taagaatatg gacggctggg gctggcgggt tcagggaacg gcaagaaggt
     3661 ctggtgatta taatacacct gattatgttt tgacaaatac cggcgtaaaa gaattgaatt
     3721 tttctgcagc tacaggatac cataaagaga aaatcggctt cgatgttttc tttagccatt
     3781 tccagactca aataggaata ttgaaaggta cagcaatagg ttctcctgaa gacctggcca
     3841 cagcaatgga atcgccaata ccacagtaca caacagattt ttcctataag attggcgagc
     3901 ctcgacagga agtaagtcat aatctgctca aaattaacgg tcatattcaa acaactcatg
     3961 gtgactggag gatacaatat ggatttcaaa ataataatag aaaagaatac gatatacgta
     4021 ttggtcagct atcccaaatt ccatctatca atctaaagct aaattctcac agcctggatc
     4081 tggaatggga aacgcttcat tctgaaaggg gtacggttat tcttgggttg aataatatcc
     4141 tgcagaataa ttataatata tctggcaccc agagaatccc gtttattcca aacttcacaa
     4201 atctctcaac tggggttttt ggagtagcta agctattgtt gaaaacatgg actatggatt
     4261 ttggcctcag atatgattac agatattatg atgtcaccgg atatgatttt aaaaacagct
     4321 tgtatagtac tacgtataat ttccacaaca ttagtgcaac tcttgggaca aaagtttcgc
     4381 tggctaaaga ccatgtcatt cttctaaacc tgagtagcgc ctggaggcct cctcatgtag
     4441 cagagctcct gagtattggc aggcatcaaa gcgccgcggc aattgaatat gggttgctgc
     4501 tccaggatac aaccaatgag gttagaagta tcggggatgt gaatttcaac acggagcagt
     4561 cggttaaatt tgtcgctact tatcaatgga acttacaaag ttttagtctc gaagccagcc
     4621 catatgctaa ttacatattc aactacattt ttctgcgtcc ctacggcgta accaaaagca
     4681 taagcggcac ctatcccttt tttagataca cccaaactga tgcactattc ctcggaatcg
     4741 acctttcggg atcctggcgg ccggtagcga atttccaggt aattccaaag gcctccctgt
     4801 tgagggcaag cgatgagcag aataataacc cactggtttt tattccatcc aacaaatatg
     4861 atttaacctt taggtatgaa aaacccgcgt ttctttttat taaaagtttt tatatagaat
     4921 ccaaatcaat ctatgtagca agacaaacca gggcgccccg ggttatttcg ccacgcgaaa
     4981 ttgtggaggc ccaggtagaa ggtgtcgatt tgttcaaaga taacacctca atttttgatt
     5041 ttcaggcggc acctaaagga tattttcttt ggaatatttc tataggcgct tccatcagac
     5101 gaagtcatgt tcaatataat ctgcgcattt cttctgaaaa tactttaaac caaaaatata
     5161 gagaatacac caaccgcctc cggtattatg cggatgatat tggacgaaac atcactctct
     5221 ctttaaaatg tattttctga aaaaataaac ttaaaactat catgaaaaat aaaaaaagaa
     5281 tcaaacacaa tctaaatctt ctttcagtaa gtctgatggg agtatgtctt ctgatttttc
     5341 ttttgtctgg atgcaaggat aacgatttag taaaagaaga tgtacctgaa ctaataacca
     5401 aagcaagttt aacctttact ccgtcaggcg cagggaatgt aattgtggta acagcaaccg
     5461 atcctgatgg ggaaggagtg caggatattt cagttgatgg accgataaat ttaaacgctg
     5521 atgaaaccta tattctcacc ttaagcctta ttaatgagct ggcagatgca accgatcctg
     5581 agtacgacat tacggatgag gttgaagcgg aaggagatga acacatgttc ttcttctcct
     5641 ggacgaacaa tgtatttgct gatccttcgg gagatgggaa cattgataac cggagcgatc
     5701 ccgttaatta tacgggagct tcaaattctg tggatgttaa taatcgtccc cttggtttaa
     5761 caacaacctg gattaccgct acaggatcat ccaccggcac tttcaggatt cttctcaagc
     5821 atcaacctga acttaagacg gatacttcaa attctattat tggggagact gatttggata
     5881 tcacatttac cattaatatt ctctagtgaa attcagaaca gattaagcaa aaagatcaat
     5941 gcgggcatga gaataattga aaatgatcaa gctactgacc aaactgcatg gtaaatcaat
     6001 tagtcttgaa ttcccaaatt gagactttat cctcattatt ggactatttc taagaaatta
     6061 aagtgaaaaa accgtcaaat tggtctgatt taaccctttt tagtttctgg tatagcttct
     6121 gccttataat gtgcatgaaa agcgaagtta tgaaacccga cgaaatgaat ttattcaaaa
     6181 tccttatcgg attgatttac atggtattga ttgctttgat aattgtggcc gtaataatct
     6241 ggtaaaaggt aagttagcta tgatattaat tcttatcctt ctttttatac tttccagact
     6301 taaggtcctg aataaaccta acccagttga aggggtttaa gaatggattg gaaatgcctc
     6361 catatcggtt tatctggtaa tatagctgct gatctaaata ataactggcg cttaatgaac
     6421 catccaatgg agtattctta atcatatacg ccaggtattc gccattaagg ctttccctca
     6481 gttgcttggt ttcatcagga agtcgcaacg ccagccatgc ctgcttgagc gcttcctccg
     6541 tcggatatgg caggatgtcc acattctcca ggaatattgt atcaacctca agctgaaaga
     6601 gttctgtgat atcatcaccc tgattaaatg gaacaataaa attttgttta tgaaacccca
     6661 ctgcactgat gattagacta tctcccgtca atacagggag tgaaaaatat ccataggcat
     6721 tagtggttgt acccctgcct gccgtaggta cataaatgtg cactccgggc aaggtggctg
     6781 tactgtcctc tccaatcaca attccgctta attgcacaat cttgcgttcg ggtttatcct
     6841 gtgctaatcc ggtgattgca atcaccatta atgaaatgac caataaatac cttaatacag
     6901 ccattatttg ggtttttccg gttacaaagg tatgaaatag ctcatagaaa gtgacccctg
     6961 tttccaagat ttaccaaaac tagcctcaaa aattatactc atttcttaaa tttgaagtct
     7021 gaaccctgat catgagactg aaaaacttaa gtttgccctt ttccgcgcag gtctttaaaa
     7081 gtttctccga agaaccccgc gttaggatta tcaatttgct ttttcacaaa gaagaattat
     7141 gtatttctga tattgaacaa attcttgaat tcacacaaac taaaaccagc cggcacctta
     7201 tctatttgaa gaatgccgga attgtaagct atagaaaagt ggatcaatgg gttttctatt
     7261 acatcaagga agaagtgatg gatcttgtca gtcgtatcct cgatttcctg cagaaagacc
     7321 aggtactaaa ggctgatctt actacctacg aaacattatt ttcaaaccgc gaactctcca
     7381 tcaaccgaat taaattttca aaattaaata aggcctaagt gagcaactac cagcatcttt
     7441 tttttgatct tgatcacacc ctttgggatt atgaagccaa tgcagctgaa gttcttgacg
     7501 atttgtatca ccgacacact ttgggaaaaa taatcaattc aggcagcaga cattttaccg
     7561 aagttttttt tgaaattaat aaccgtctct gggaccaata tgaccgcggc ttgatccata
     7621 aatcagtgat tagaaatgaa agatttgata gaattcttgc cgctttcggt gtttcaaatt
     7681 cacgtcttgc caaaacaatt aacctggcat ttatttcaga atgtccttat aaaacgaacg
     7741 taattcctgg tgccgtagaa ttactctctc atcttagacc aaaatttgga attcacatca
     7801 taaccaatgg attcagggaa atccaatgga ttaaaatcgc taagtcagga ttggataaat
     7861 ttatcgacca ggtttttatt tctgaagaaa ttggtgcaag aaagccagat cccaaatttt
     7921 tcgaattttc tttaaataag gttgaatgta cacgggcaca gtcggttgta attggcgata
     7981 atttaaacac cgacattaag ggtgcccggg attatgggat agaccacatt ttctataatc
     8041 caaagaaact aactcatacc gacagggtta cctgggaaat aaatcatttg tctcagatcc
     8101 ctgaactcat ttaatgattt ctaggtcatc tccaacgaat ccattttatt tgcactaata
     8161 aaccccgatc ggatgtcaac tggattttat acaatacccg agcccaaaaa tgaaccaatt
     8221 tttgattacg cgccaggcag catgcaaaga ggcgagttga agaaagccat tgaaaaatta
     8281 cgctccggga aagcggatat tccaatgtat ataggcggcg aggaagttcg gaccaataac
     8341 aaaatcagaa tttctccacc tcacgaccat aaacacactc tcggcttttt tcatgtgggc
     8401 gataaaaacc atgtggaaca ggcggtgaat gcggctttga atgcaaaatc atcgtggact
     8461 tctatgtctt gggaacaaag agcggccatt tttctcaggg cggctgattt ggtttctgga
     8521 ccttacagat atattcttaa tgcggcaacc atgcttggcc aatctaaaaa tccattccaa
     8581 tctgagattg atgctgttgc agaatatgca gatttcctgc ggtttaatgc tcattatatg
     8641 gagcagattt acaaagtcca acctatttcc cctgctctta cctggaatcg gttggaatat
     8701 cggcccctgg aaggtttcgt atttgccatt acgccattca acttcacaag tatcgccgga
     8761 aatctcccgg cttccgccgc cttaatggga aatacagtag tgtggaaacc cgcatataca
     8821 caggtttact ctgcaaatat tctaatgaag atttttattg aagctgggtt acctcgagga
     8881 gtaatcaatt tgatttatgt ggatgggcct gtagctggtg atgtcatctt cgaacatccg
     8941 gattttgccg ggattcactt caccggaagt acctcagttt ttagaaaact ttgggaaacc
     9001 attggtaaaa acattgctaa atataaaagc tatccaagga ttgttggaga aacgggggga
     9061 aagaatttta tcattgctca tccttcagca aatgcagcgc aattgtcaac ggctattgtc
     9121 cgtggagcat ttgaatacca gggtcaaaaa tgttcggcag cttcccgcgc atatatacca
     9181 tcatcattat ggcctgccat taaggaaaat gtttcgaagg ccctcaagga aatcaagatg
     9241 ggcggctgtg aagattttag aaacttctgc aatgccgtaa ttgatgaaaa agcttttgat
     9301 aagatttcag catatataga gaaggccact tcaagcaacg ttgcagaaat aatagcaggc
     9361 ggcaaatgtg ataaatccaa gggatatttt atcgaaccaa ctgttatcct ggctcatgat
     9421 ccccaatttg caaccatgtg cgaagagatc tttggacccg taattaccat ctacgtttat
     9481 aatgatgata actgggaaga aactctggac cttgttgatc aaacctcacc ttatgcactg
     9541 acaggagctg ttttctcaat cgaccggcag gccatacaac tggcaaccaa aaaactggta
     9601 aactcggccg gaaattttta tatcaacgat aagccaaccg gagcagttgt tggtcagcag
     9661 ccatttggtg gagctcgggg gtcaggtacc aatgacaaag caggttccct ttttaattta
     9721 ttacgatggg tctccgtcag aacaattaag gaaaacttcc ttccaccttc ggattaccgc
     9781 tatcccttta tgaaagaaga gtagttctca gaattctcag tccccccttt ttcggtaaaa
     9841 tactaaaaaa cattaccttt acttgaattg tataaaaaca atatttattt ttgatattct
     9901 aatgaaaaaa gttgaattta tcaatactaa ttgttatttc tatattattt tttactactg
     9961 tagtagtata caatcgggtt tttctaagat tcaagatttc ccaattcagc agggcagaaa
    10021 aagatcggca cgcaggcttg aacaaaataa ggaaggaaat ggccagggat tttcatgatg
    10081 aaatgggaaa tcatatggca agtattatca gtatggtaaa catcttatcc atgaaattga
    10141 aaacaaatga ccagcagatt cttagcatct tgaaaaaagt tgaatttgct tctaaagaac
    10201 tgtttcaggg aactcgtgag ttcatgtgga ccatcgatcc ggatcatgat aacctggtgg
    10261 agatcctgat ctaccttaga gactacgggg tcgacttttt tcaaaatact aatattgatt
    10321 ttgaagtagg caagaactta ttgaccggcg ctgaggcatt accggtacaa ccgggcagct
    10381 gcaagcacat ccttgcaatc tttaaagaag caatgacaaa ttgcctgaaa cattctcacg
    10441 cggctaaagt gacattatca tccgaatcca ccaaccacag ctttacaatt atcttttgcg
    10501 ataatggaat cggtgtgcgg gaaaagatgg gacgcggact tcaaaatatg caaacccgag
    10561 cattaaaagt aaatgcgata ctaaaatgtg aaaatatgga gcagggattt tcagtggcct
    10621 tgaaattcaa taacttaaaa atagtcatat aaataacttt aaaataacca aatatgaggg
    10681 aatctagatt caactacaac acacttgacc gccgtacaag gattgtaatc attgaagaca
    10741 atgagcaaat ccgggaaggg tacgctttga tcttaaatac caattccaat tacaatgtgg
    10801 taagcactta taataaatgt gagaaggctc tcaagtatct tcacaaagat aatccggcca
    10861 ttgttttcat ggatatcgag ttgccaggta tggatggtat tagaggtatc aaggagatca
    10921 agaaattgag gccggatatt gatatagttg taatctccgt ttatgatgaa aatgacaaaa
    10981 tctttgatgc cctttgtgca ggtgcatccg gctatatcac aaaaagctca aaccacatgg
    11041 agctgttaca cgcagtagat caggttctta aaaacggcgc acccatgagt tctaatatcg
    11101 cccggaaagt agtgcagtca ttccagagga attacaatac tccactctcg gcaagggaaa
    11161 cccaggtact ttccttcctg gcatccgggg caacctacca aagcatttcc cgggatcttg
    11221 aaataggatt agagacggta aagacccacg tcaagcatat ttatacaaaa ctacaggttt
    11281 cttcaaaaac agaagctctc gaattagcaa agaagggcaa ctatatttaa gaactacttc
    11341 atttttctat accccaaagg gtgactcatt caaatcttcc gaatcatgag tcacccttat
    11401 cccgtgattc gccgcattaa tctaattccc ttctttgtgg tgttgcacct gtgaaaaaca
    11461 ctgcgctatt ttgaaagcag aaaaattaag ataacatttt caggaactca ttacgtaaat
    11521 gaaaaacaat tttagattac taaaccatcc tttctatcag gcttggtcac gtggtaaatt
    11581 gactccaaat cagatttcaa tctatggttc ctcatatctt gaactcatag agcaaatccc
    11641 aacttattgg caaaaaattc tccattcctt tggaatcgaa gataccctcg atgcacaaca
    11701 aatcgtaaag gaggaaaaag aacatattaa tttgtggaag aaatggatta ttaaattaca
    11761 ggagccaaaa gattttcctt ctatgaaaga tgtaacagcc tcattattca aaatttctga
    11821 gtcggaatta ttgggagcaa tctcttcatt cgaaatgcag cagccgaagg ttgctaatac
    11881 aaaaaaatcc ggattattag aacactttgg ttttgaagcg gaagatctga cttactttga
    11941 tgagcactta aaagaggaga aacacattca atttgcgaaa agagtacgag agcattattc
    12001 taatcactcg gaatttcagc aagggtacaa ttttggaaag aaattatttt atgaggcatt
    12061 agacagattt ctttccaatt aacttataaa aagcataaaa aatttacccg aataaaaatt
    12121 tataagtaat aattattttt tggaatatct acgaactatt cgacttgttc taagcaattt
    12181 gaaattgaat ttcactttac aagtcctgat tatcagggtg gatacgatga taccttcaca
    12241 ataaaatata actttaaatt atttgctcgt tgcaaccaaa aacaaaaaag gccggctaac
    12301 aggccttttt tgttttgcaa tattctaaag aaatttggaa tctaatccct aattaatagt
    12361 taaaataatt attgctattt caagaattaa acttaatcaa ttccacaaat cttggcacag
    12421 tgatgaatta gagcaactcc attcaaattg ctagtaaaag ataccattca tttcatccct
    12481 taaaaccagt gattcattgc actacttttt tgttcacctt tggccgttta acaaaaaata
    12541 aaaagcatga agaaaaatct actttatttc ttatgggtgc tttgtcttgt aatgattcaa
    12601 aacaaagcaa gcgcgcagga acgcgtggtg tccggcaagg taacttcatc ggaagatgga
    12661 tctcctatac cgggagtcaa tgtgattcta agtggaacca caacgggtac cataacagat
    12721 gcggaaggta cgtatagaat gtcagttccg caggagggag gcacaataat tttctctttt
    12781 gttggaatga aatccattga agttgcaatg ggttcccagt ccgtgataga tgtgcaaatg
    12841 gaatcggatt ttcaggaatt gggagaagtg gttgttacag ctcttggttc tgaaagaaat
    12901 gccagttcca ttgtgtatgc caaccagacc gtaaaaggtg aagatttatt atccactccc
    12961 aataagaatg ctttagaagc attgcgtgga aaagcagctg gcgttaggct atcaaccggg
    13021 tctggatcgg ttggagcttc tactcgtata gttctaagag gtgaaggttc tcttacaggg
    13081 aataacaacg cgctaatcat tgttgatgga attgcaattg ataacacctc aacttcgggt
    13141 ggtgcgggta cttctactgg cggatatgca gatcatggaa atcgtttcaa tgatattaat
    13201 ccggatgata tagaatcggt tactatcctg aaaggtcctt ctgcaacatc gctttatgga
    13261 tcaaggggtg cgtctggtgt tgttttgatc acaactaaga aaggaaaaaa aggagatgtt
    13321 caagtaagat tgaactcttc ttattcccag gaaaaagctt atattctgat gcaaagacaa
    13381 gacagatttg ggcagggata tgacaatgcc cactttgatt ctggagaaaa ctggtcttgg
    13441 gggcctgaat ttgacggggt tgttagacca tggactagcc cgattgatgc agatggagat
    13501 ggttcactcg aatcattaat aagaccttac agtgcggttc ctgatcaaat tcaagaattt
    13561 ttcaatacag gaaatacttt atctaatagc ataagttttt ccggagccaa tgaagggttt
    13621 acttactatg cttctttttc taatgtaggt caaagtggta ttctggataa tacaaagtac
    13681 aagagaaaca cagttaactt caatgcaacc gcaaaattgt ctgaaaaatt gagttctgat
    13741 ttcaaaatat cttatgcgag tataaatcaa aatacggccc aggaaggatc aagagccttt
    13801 gaaggaaaca atgcgtatgc aatggttctt cagtctccgg tgaatattcc ttttggtgaa
    13861 ttgagagatt ataatagtcc cttccatgga attgacggtt actggggttc ttactcttcc
    13921 gttaacccgt attttatctt aaatgaattt ggtaatgaag gaaagatcaa taatgtcctt
    13981 ggaaatgcct ctttaagtta caacatcatt gaagggttgg attttactac aagattcggt
    14041 tccaatgttg ttagcacatc tgtggagact tggacaccta agtttacgcc cagtacacag
    14101 ttgatatggg gcgatgattt agaattagca actagaaata gcaagcacgc atcccctggt
    14161 gattatacct atttccatgg cttgactatc aatagagatg ttactgcaac acttaattat
    14221 atgaagaacc ttacggatga tataagtatg gatatttctg cagggtacaa tttcttccag
    14281 agagagagaa agaattctac aggtacgact aatggtggtt tgttggcaga aggcatatat
    14341 aatttgggta atagtgtagg taacccaatt gcttcactgg aaagaagaaa gtatagaatt
    14401 tatggagttc ttgctaatgc caggtttgga tttagagatg cattattcct tgagtattcc
    14461 gtaagaaatg actggtcgtc tacacttccg gagaatagca atagcttcct ttatcagtca
    14521 gttggtacct ctgtagtgct aaatgacctg attggaatgg atgataatat tttgaatttt
    14581 gctaaaatcc gtggaagtat cggtacatct ggtaaggatg ctggattgta cttattgaat
    14641 tcatcattca caagtaatcc cgaagtattc tcaggtgata atccggtaag atttccaatt
    14701 aatcaacagc caggctcaac tgtgggaaat ctaattggca gcccaaatct aaaacctgag
    14761 cttacgctga cttatgaatt tggatcagat ttaggatttt ttggggagcg aatcgaaata
    14821 tcatacacgt attataaatc tgaacacagg gatcagattg ttgaggttag cttgccaaga
    14881 tccagtggct ttacaaccac tgccagcaat attggtttga tgactaacaa aggtcatgag
    14941 cttacactct cagttaaacc aatttatggg gtggtagatg gccttgatgt tgaacttttc
    15001 ggatcatggg ctaaaaacgt caacgaggtt gtgaaggtag ctgatgatat aagcgaacta
    15061 tcttttggaa cctatacccc aggggtgaca gtggttgcta aagaaggtga accttttggt
    15121 acttataaat cattggcacc tctgactaat caagatggac aagttatagt agatcctaat
    15181 accggttatc ctctttacac cgagcaagag gtatacctgg gatcttatca gcctgatttt
    15241 atagccagtt ttggtatgaa tgccaaattc aaaggaattg gtttcaacat cttatttgat
    15301 atgaaacaag gaggaaactt cggatctcaa accaaattct tcactgaatt caacggaact
    15361 tctgagcata ccgcaattta taacagggaa tcatttatat atcctaattc cgtaattgaa
    15421 aatgacgatg gaacttattc cgaaaatacc atcgaaatta cggagcagga tctctttaca
    15481 aactacgacc ccgcagcttc aacatatatt attgatgcaa gcttcttgaa gctaagagaa
    15541 attgggttga gctatgattt gccacggaat ctgatgggaa agattccatt ctcttcagcc
    15601 aggatttcct tgtttgccaa gaaccttaag ttctggcttc caggtgagaa tacttttgct
    15661 gatcctgagg ttaacggacc agctttaact ggcaatgctc agggtattga gacatctcag
    15721 actccatcat ctaagagctt tggggttaat ttacagttga cattttaacg taactaaaaa
    15781 gaaagaaaca atgaaaaata tatttaagtc atcaatagct tttacgctga tcttattcat
    15841 gtcgtgtgaa gaaaagatca ccgaactaag taatgatcct aatgtctccg cgattgctaa
    15901 cgattctgag gtactaacaa gtgccattgg atatatgggt gcaattcagg atgtagacct
    15961 taaccaggga tcatttttgt gggcacaata ctatacgtgg ggaataggcg tatcaattgg
    16021 taatcaagag aggtatgtgg cgcagcctga tgacagaaat ggatactggc aaagagcata
    16081 tgccaactgt ttggctgacc ttaattattt aagaagcagt gaaagcgcag catatagagg
    16141 agtcgcgggc attttgactg cctatgtttt tcagggtctg gttgatcact ttggtgatgt
    16201 tccctactct gaagctctac aaggagagaa tggagttctg actcctgctt tcgattcagc
    16261 tccgacaatt tacgtttcct tggtgagctc gattgattct gccatggatg aactggcatt
    16321 agctaccaat gatattggag atgatgatct tgtttatggc ggcgatctta atcaatgggt
    16381 gaagtttgca aactcgctta agctaagatt gttgatgaga acttctgaag ttgatgcaaa
    16441 aggttcagag ataactacac ttgtcagttc cggaacattt ttagagtcat cagcagacat
    16501 tgctcagatt tcttggtctg gaaccactgg aaattcgaat ccaatgtggt ctgtgcaaat
    16561 gaatggggtg ggcgactttt actttgcaag taatgccatg gtcaacttgc taaatagtct
    16621 aaatgatcca cgattaacca ctttctatgc cgctgccaca acgggtgcat ttaatggtca
    16681 gattcgagga attaatcagg gaaccataga tgatgaacca tttacagctc ctgcaactga
    16741 ttatagtggt tcttctgctt atgccaatgg tgctacggct cctacagttt tgatgagccc
    16801 ttgggaagtt tggttcctta gaactgaagc agcagccaga tacggtaccg ctgataatga
    16861 ggcgacagcc tttgcaaatg cagttcaggc gaattttgat tatatgggta ttgcaggtgg
    16921 tgcggcttac attacctctc tcagctattc tactacagat cctttgaatg atagattaaa
    16981 tacgattggt attcaaaaat ggatttccat gaatggaacc caggaagatg aaggatggat
    17041 cgaaacgaga agatttgatc gaccgggtga tcgaattttc actagtggaa tattccaaaa
    17101 tcctccattg tctgttttag cggctggaac tttccctgct tcctggttgt atccggccac
    17161 agagagaagc tataacccaa aggctcctgg tcagagggct ataaccgatc aattattttg
    17221 ggataactaa aaattaaaaa atatgaaata ttatatatta ttaataattg cgcttgttgc
    17281 cttttcttca tgtaaggaat atgagaatag tgcagagcca tcttccgtaa catatttgcc
    17341 tttgattgaa attcagggtg aacctgaaat tgatttggaa tgtaatgtgg gcggatatac
    17401 agatgaaggc gctgtagcaa ccgaaggagg aacttcgatt gaactaacta cctcagtcat
    17461 tggggtctat ttcgaaggaa cggccgtaaa cattccggat ttataccata ttacatattc
    17521 agctgaaaat gtggatggta tcccgggtgc agagactaga agtgtaacct ggccagcatg
    17581 taatagtgat ctggtaactg gtattgaggg aatgtatact gctgcggtat tcagaactgc
    17641 tacttctgga gctacagtag attattctga tgcagaacac gggccatatt gggttgtgga
    17701 tcttggtgga ggacaatatg gattttcttg tgtgataggt caattctatg aatatggatc
    17761 tgattacggt tatggccctg cttatgccgg taccgggttt gtagtaacag caaatgatat
    17821 atcaacaaac gacttcacta ttacagcaac taatggcgtt ggagcatttg gaagggatat
    17881 tgaagttacc gattttacgg tagacagtag tgccggaact atcagttgga ccgctacatg
    17941 gtctgctgga tatgaatttg atgtagttct tactcagaat tagtcattta tattcgatat
    18001 agtgaaagta aggtgtctcg aaagagacac cttcttttta gtgtgtcagg catgtcttaa
    18061 tcagtgcctc attttcccaa agggacaggc gtttctatta atcaggtaat gaaataacat
    18121 taccttgaag ctttaatcct tacatttttc ttcgcaactt atgaacagcc ccggtttatt
    18181 ttgctaggat gtagaaatag gcggaaagga tctggtggtt tgaaagcctc gggttagttt
    18241 caaaaccaaa atcagcctaa tcccttatac atacgaattt taaacaatac atattatatc
    18301 aaaaaaggct gggaatacca ccttttttga ttatctataa ttccaaatct ttatcccaaa
    18361 tattcagcaa gtaacttgct tcgtgagctg tgaccagcct tcgcaaagtt ctctttctcc
    18421 ctggataatt tttattgacg ctaatgtgtg tttacaattt aagcagtcgg gtagaatggt
    18481 gaactgttaa aatatctatc cggtcgtcct ttgagtccaa gtaaattatt cggtaattac
    18541 ccaataagac ctccctgatt tccagattat taagttcgcg tacgattctt cctgaatttg
    18601 ggaaatttgc taatcgcctg gctctttcac gaatccgagt tatctgaaac ctggcatatt
    18661 agttgaatcc tgagctatga actctgcaat cgagattaga tcatcttggc ttgaagtgtt
    18721 caatttagct tagccattca ggaagttccc ggtcaagtac ctcgtccgaa attacttcat
    18781 tagtctctga ctgagctaat cccttttcta tctttctcaa gcaatataag ttcgtcaatc
    18841 acttcatcaa tagaaaattt gccaggaagt tgatttattg cacttataac ttgttctttt
    18901 gttagcataa tctaagataa tggaagtaaa tatgatgccc aaatagtgtg ccatgcttca
    18961 cagtgattgc aaagccataa gcgaacggga gttttagcaa ataaaaattc ctgagtgtca
    19021 attttttctt tacacctaac gaattttgca gcaaactttc tttgactgcg caagggtctg
    19081 aatcttttga aatgtaattg tgaattgttt cgagatcggc aattgcgcct tgaattgaga
    19141 aatattggca tattcataat aaaaaaaggc cggtaaaacc ggcctttttg cttatccata
    19201 attattccaa atctttatcc caaatactcc gcaagtaact tgcttcgtga gctgtgacgt
    19261 aatctgcgta atgctttctc tttgatttga cgaacacgct cacgggtcaa accgagaatt
    19321 tcactaatct gttctaatga catcgggttt tcttctccaa taccaaaaaa catcttaata
    19381 atctgagatt ctctttctgt tagcgttgat aatgcccgct cggtttcaat actcaaagaa
    19441 tccttatagg taatcttttc gtccgttctt tccatactgt catccacgag cacatccagt
    19501 aaactgctat cttccccttc ttcaaaagga gtatctactg acaactgacg agtggtgatt
    19561 ctcattgtat tcttgatatc atcctcagcc atatcaagca gattagctaa ttcttcttca
    19621 gtcggcattc tttccagtct ttgctccaat acagaagcag cctggctaat cttactgatt
    19681 gtgcccacct tattcaaagg cagacgtacc aatcgtgatt gctcagctag tgcctgcata
    19741 attgactgtc gaatccacca taccgcgtaa gagataaatt tgaatccacg ggtatggtca
    19801 aatttctgag cagcttttat taaacccaaa ttaccttcat taataaggtc atttaaggta
    19861 agattcctgt tttgatattg ctttgctacc gaaaccacaa accttaggtt tgctttaacc
    19921 agtttttcga gagcagcttc atctccttcc tgaatccttt tggcaaaagt aacctcctct
    19981 tcaatagtga gtagatcgac cttgttcacc tcctgaaaat acttctccag ggattggttt
    20041 tccctgtttg taatgttttg tgtaatcttt aattgtctca taatttattt tactccctat
    20101 actaaaatct ccaaattcct cctcacctta aatcccctta aaaacggatg caaaggtagc
    20161 taaaagaata acgcaagtca agtaataatg ttttgtaatc aggtaaataa agtaatgaaa
    20221 atatccctga atttgctaaa aaaagctggt tttcccaaga ataatccaat tcctatttat
    20281 atatacgttt tttagcattc tgtgttaaat ttacaatcga ttaaaacact aaatgccctc
    20341 ctcacttaat acggctatta ctttaatggt aattgggatg accacggttt tttttatttt
    20401 ggccctggta gttggatctg gtaaaacctt aattctcttc gtaaatagat tttttccggc
    20461 tgaaaaagtt agcccgattc cggtattcat agaaaaaagc accatttcca aaactaagat
    20521 ggcagttata attgcggtgg tggaaaagat tacaaaaggg gaaggacaga ttttagatat
    20581 aaaaaagtta aataatgggc cgaaaaatta ggtattgcct tgtgtatagg gatatgtggc
    20641 agtcgtccgg gaagtatatg ccaagggctg atcaattagt gcgagtggca ggtcccatca
    20701 ttgatatggg ctgcttcgcg agggtagaaa caaatggagg tggatttgaa caaataaatc
    20761 ttttattcgg tgaaaaccca aacaccgctc ttcgtcagtg gacgaaatcc ttcaatgaag
    20821 taggtataca aactcaaatg ctggagcggg gattaaatgg aataagaatg tatccggttc
    20881 ctgcagatgt gcgaagaatg atgttccacc tgaaaaagaa acaaggtact gatattgctc
    20941 gttctttcga tggattaaat gatgtgcaaa atctcgaact ctcagttagg tatgcattag
    21001 agggtgggat gattcctcag ggcgcccttt gcattactca ttcaaaagtt catactatcg
    21061 aatattatct taagatggcc gacgacctga ttgaaatggg agcaacagag attggcatta
    21121 aagatatggc tggtgtcgga aggccggttt cattgggaag aattgtaaaa ggaattaaag
    21181 atcgacatcc caaaatcctg gttcagtttc actgtcattc aggtccagga ttttctgtgg
    21241 cctcaactct ccaggccgtg gatgctggtg ccgacatcgt agatgtagcg atggaacctg
    21301 tttcatgggg aacgggtcat gcagatttgc ttaccatcca cgctatgctc gaagatgccg
    21361 ggtatgaagt tcccaaaatc aacatgaatg cctatatgaa agtcagaagc ctgacgcaag
    21421 aatttattga tgatttcctt ggttactata tgagtcccaa aaacagactt atgaactctc
    21481 ttttaatagg atcggggtta ccaggcggaa tgatgggaag tttgatggct gatttagaat
    21541 taaaccttga aagtgtaaac aaatacctta cgaagagtgg cgaactacaa cttactcaag
    21601 acgacttact cattaaactg tttgatgagg ttgagtacat ctggccaaaa ttaggatatc
    21661 cgccattagt aactccctat agccagtatg tgaaaaatgt tgcccttatg aatgttatcc
    21721 aattaataaa gggtagagag agatggtcga tggtatctga caatacctgg gatatgatat
    21781 tgggaagaaa tggaaaatta cccggtccgg tggatccggc cctggttgaa ctggctacct
    21841 ctcaagggaa agaagttttt aatggcgttc ctcaggattt gtatccggac aatctcgact
    21901 tattcagagc ggaaatgaag caaaattcct gggatttagg ccaggatgaa gaagagttgt
    21961 tcgaattagc catgcacccc gagcaatacc gagcttataa atcagggaaa gcaaaagaag
    22021 cttttcacca agatctcaag gaaaagaaac aagaaaagtt ggttccggtt acgaaaggag
    22081 aggttgcggt aaaaacagat ttgccaagaa cattggatat cgaagtaaac ggtgaaaagt
    22141 tccaggtgaa aattggttat cctaatgtcg gtgctacacc aatttcttca gaaaatggat
    22201 ctgccggtca tgccgatcta cagaatctta gagagattac agctccaatt gagggaacct
    22261 ttatgctaac caaagattcg gcggagaggc caattaaagt tggagatatt attaagaagg
    22321 gagatctgat tggctacatt gaatcgatga aagtatataa tgctatcact gctgataaga
    22381 atggggaagt ggttgagatt tgttcaagaa acggtgatca ggtgattgaa gacgaggttc
    22441 taatccgaat tgcataatcc atggagatcc tgaggagaat ttatgaaatg accgccattt
    22501 ctgaggtcat taaacatcct gcagtgattc tgatgtgggc gataggattc ttcctgcttt
    22561 atctgggaat aaggaaaaaa tatgagccac ttttattagt gcctataggt ttcgggctaa
    22621 tcctggcaaa tgcccttggt ggtgagatga acattgttca ggatgatcat ttggcagaac
    22681 ttccattagt aaaaattgca aaggatcacg ggataatgaa ttttctgtat tactctctca
    22741 taaaaaccgg atttttaccg ccgataattt ttatgggagt aggggcatta actgattttg
    22801 gcccgatgct gagaaatctg cgactggcgt tctttggagc agccgcacaa ataggtattt
    22861 ttacggtatt atttgccgct atcatttttg gcttcacacc gaaagaggct gcaagccttg
    22921 gaataatcgg gggagctgac ggtccaacag cgatatatac tactatcatt cttgcccccc
    22981 acttgctggg gcctattgcc attgctgcat attcttacat ggcactggtt cctgttatta
    23041 ttccttttgt ggtgaagtat acaaccaccc aaaaggaact tcttataaat atgaaggaac
    23101 aggacctttt atacccggaa aaacatccaa taaaaaatat tcagttgatg aaaattctat
    23161 ttccgctttt ttgcggtgga ttggttgcgg ttttagttcc taccgcggtt cctttattgg
    23221 gtatgttctt ttttggaaat ttaattaagg aaattgggcc cacaaccgaa cgactctccc
    23281 aggcagcttc cgggcctatt atgaatactt cgacaatatt tcttggactt tgtgtgggcg
    23341 caaccatgaa gtacgaaaca tttctggata cgaaaacact gctaataatt gccggaggtt
    23401 tgattgcctt ttgcatttcc attgcgggag gaataactgc agttaagatt tacaatgtct
    23461 tttctaaaaa gaaaataaat cccctgatag gggcaacagg cttaagtgcc gttccaatgg
    23521 ctgcaagagt agccaatgat attgctttga aatacgataa gcagaatcac ctgcttcatt
    23581 acgcgatggc cagcaacatc agcggtgtaa ttggttctgc tgttgctgcg ggggtgctaa
    23641 taacattcct cagataatta cttaactaaa ccttgcagaa cccagactac gtaaggatga
    23701 ggttttttat ttagtattct tctcctacta tttcaaacca agagaccctg tgatgaaagt
    23761 gtcgtggtat atgaagcgtt gtctctcaat gattttctga tgattaataa tatttctgat
    23821 gattaataat attgaaaatt tcccactcaa taagatagag atattgatag ttggaacaat
    23881 cttgtattta gtcaaaccct ttacaacaaa tctaacaatg tgtggcagga agaatccaat
    23941 catttcagtt tcggggaaat cactcgcaga aggaacttac tttatgtttt agatttggga
    24001 tacaaaagtt tgttacgtag aggttatctt ttattgaatc ggtaagtaaa atgggaaaaa
    24061 gggattgcgt attgtaaatt aataaggttg gccgtaatat tgcattccta aaaagacact
    24121 cccccttagc tcagttggtt agagcggctg actgttaatc agtaggtccc tggttcaagt
    24181 ccaggagggg gagctagaaa aggccatttt atttcaaatt tggccttttt tattgctcgt
    24241 tattgcgtat ctttaagaag taacgggctg aaaatctcga ccaaaactcg acctttcaca
    24301 attttctcga ccattactcg accatagttc gacctgattt ttctgcagta aacttttaaa
    24361 catctgttat gtcagcatat gtaaaattgt actgcaagaa gagcaggctc aaaaaaaatg
    24421 gtgaagctcc catctactac gtaattaaac tcaacgcgaa ggaaaagctt ttgttttccg
    24481 gcaagacaat caaaccttca ttgtttgata atgaatccgg agaaattaag aacaaggtcc
    24541 tatacggaaa attaaattcc tgtctgacga cagagaaggt aaggttcaat aatattatcc
    24601 tggatctgga atacaagaaa gaaaccataa ccttcgaaaa gatcctttct tgtttcaaat
    24661 cacctaccga aaataaatgt tttattgaat tcttttcgaa tgaattagat gcaatcaaaa
    24721 atgggatcgc taagaagact tataaagatt acctggtatc gttaaataat ttgaaagagt
    24781 atagacaatc actttca
//
LOCUS       DASWHX010000166         3397 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_2042776, whole genome shotgun sequence.
ACCESSION   DASWHX010000166 DASWHX010000000
VERSION     DASWHX010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 3397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3397
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2042776"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..917)
                     /locus_tag="VGA21_11125"
     CDS             complement(<1..917)
                     /locus_tag="VGA21_11125"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9651105.1"
                     /translation="MLKNYFKSAYRNFVRHKSFTFLNVSGLALGLSASLMIIQYVKYE
                     RSFDSFHSNAENIYRIQYNNYQNGSLSYECAAAVPAVGPEMKNNFPEIIRFTRLYPVS
                     GVITYNSPELGIISFKEDKMQIADQRVFQIFDFELAEGNPETCLEGPNKAVISQSAAQ
                     KYFQDEDPIGKTIKWGSEREYEITGIMEDVPDNSHIKFNILFSFQTLNNETENASEES
                     WGWYDFNTYVQVQEGVEIKELQAKWDKHLQEVRGEAWEHNNFKQEFLFQPLLNIHLYS
                     NLLQESQPQEQGDGQAVYFLGIVAFFILI"
     gene            complement(1035..>3397)
                     /locus_tag="VGA21_11130"
     CDS             complement(1035..>3397)
                     /locus_tag="VGA21_11130"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX9651106.1"
                     /translation="INIAGLSMGMASVFLIILFINDELSFDKFHANAGDIYRIAWQSD
                     DPQTRTPHPMPLAMVEEFPEVIAGTSLSPLWAPGLTRATFAVRNPVNNVHFDEEGILS
                     VDSTFLKVFSFKLLKGNADKVLKEPGYLVITEKSAKKYFGEEDPVGKFLQVTDDERAH
                     TLEVGGVIEDVPVNSHFHFDGLISYVSLKRPGDLYYTWDDFGHYNYIKLRPGADAKAL
                     EAKLMDWAAQHIDIPEERLEYLRTNNLRFILQPLTDIHLKSQIRWELEPNGNISYIYI
                     MASAAIIILLIAGVNFMNLTTSKSTERAKEIGVRKSLGSTRNQLAIQFISESLLLAGV
                     SVIISGLLVELILPWFNSISGKALEINYFKDYNMLLWMVGIAILVGILAGLYPALIIS
                     SSKPHLILKGGAIRSGKGKWIRQALVIFQFGAAMLLITGTAVIYQQLNFIQNTNLGFD
                     KEEIVFIPIRRDEMRRELRTLKTELLRIEGVTSVSAVSNVPGKSFNQNPVYLTENPQN
                     RINASEFSTDFDIFPALNIKLSEGRLFDEKFSTDSTSSFIINRAAATSLSPNESVVGK
                     ELTWDADGNFFKGSVVGIVENFRFQSLHQPVRPVIFQIRPSSYNHLLIKMKTVNPEKQ
                     LKEIEAAWSQFENSFAFSFSFLDETINNQYRSEQRMGKVFNGFSVLAVIISSMGLFAL
                     ASLLFTQRQKEIGIRKVLGAHSSNLIIMLLKNFTVLVFAGIIIGIPISYFLMNNWLAN
                     FNVKIELSPSLFIVAALLILLISWATIGYLTYKTAKTNPVETLRME"
BASE COUNT         1036 a          726 c          616 g         1019 t
ORIGIN      
        1 attatcagga taaaaaatgc gactattccc aggaaataaa ctgcttgtcc atcaccctgt
       61 tcctggggct gggattcctg caacagattt gaatacaaat ggatattcag caaaggctga
      121 aaaagaaatt cctgcttaaa attattgtgt tcccaggctt ccccccgcac ttcttgtaaa
      181 tgtttatccc atttggcctg aagctcctta atttcaacac cttcctgaac ctgaacataa
      241 gtattgaaat cataccagcc ccaggattct tcactcgcat tctcggtttc attatttaaa
      301 gtttgaaagg agaataaaat attaaatttg atgtgtgagt tatccgggac atcttccatt
      361 attcctgtga tttcatattc ccgctcgctt ccccatttga tagttttacc aattggatct
      421 tcatcctgaa aatatttttg agccgcgctt tggctaatca ccgccttgtt tggaccttcc
      481 aggcaggttt ccggattccc ttccgcaagc tcaaaatcaa aaatctgaaa gacgcgttga
      541 tcagcgattt gcatcttatc ttctttaaaa gaaattattc ccagttcagg actattatag
      601 gtaataacac cactcaccgg ataaagcctt gtaaaccgga taatctctgg aaaattattt
      661 ttcatctccg gtcctacggc agggacagcg gcagcgcatt cataacttag acttccattt
      721 tggtaattat tgtattgaat tcggtagata ttttccgcgt tgctatgaaa ggaatcaaaa
      781 ctccgctcat attttacata ttgaattatc attaagctgg ccgacaatcc cagggcaaga
      841 cccgatacat ttaaaaatgt gaaagattta tgccggacaa agtttctgta cgctgatttg
      901 aagtaatttt ttagcatttt ccggggcgta gttttattaa gtatactgaa ttaacaagtt
      961 tttggttgcc cgaatttgaa agtgaaacca ggtattccta taattcacat taaataccct
     1021 aaatacctcg cttattattc cattctgagg gtttccaccg gatttgtctt cgcggtttta
     1081 taggttaagt agccaattgt tgcccaggag attagcaaaa taagcaatgc ggcaactata
     1141 aaaagggatg gactcaactc aattttaaca ttgaagtttg ccagccaatt attcattaag
     1201 aaataggaaa taggaattcc aatgattatc cctgcaaata ccagaactgt aaagttcttt
     1261 agaagcatta taataagatt tgatgaatgc gctcccaaaa cttttcttat accaatttcc
     1321 ttttgtcgtt gtgtaaaaag caaagaagcc agtgcaaaaa gccccataga tgaaatgatg
     1381 acagcaagta ctgaaaatcc attaaacacc ttacccattc tctgttcaga tcgatattgg
     1441 ttattgatcg tttcatccag gaaagaaaag gaaaatgcaa atgagttttc aaattgagac
     1501 caggcagctt caatctcttt taattgtttt tccggattca ctgttttcat tttgattaat
     1561 aaatggttat aagaggaagg tctgatttga aatatgaccg gtctaaccgg ctggtgcagg
     1621 gattgaaatc tgaaattttc aacaataccc actactgaac ccttgaaaaa attcccatcg
     1681 gcatcccagg ttagttcctt ccccacaaca gattcattgg gcgaaagcga cgttgcagca
     1741 gccctgttaa tgataaacga tgaagttgaa tcagtggaaa atttctcatc aaataatctc
     1801 ccttcagaaa gtttgatatt caaagccggg aatatgtcga aatcggtact aaattcagaa
     1861 gcattgatcc tgttttgagg attctcggtc aggtaaaccg ggttttgatt aaatgacttc
     1921 ccgggaacat tcgaaacagc acttactgaa gtaacgcctt caatccgtaa caattcggtt
     1981 tttaacgttc ttagttcacg cctcatctca tctctccgga ttggaatgaa tactatctcc
     2041 tctttatcaa atcccaggtt agtattttga ataaaattca attgctgata tattacggca
     2101 gttccggtta tcagcaacat tgccgcgcca aactgaaaaa ttactaaagc ctgccggatc
     2161 cattttcctt ttcctgacct gatagcccca cccttaagga tcaaatgcgg cttcgaagaa
     2221 gagattatta aagcgggata gagccctgca agtattccaa ccaggatagc aattcccacc
     2281 atccataaca gcatattgta atccttaaaa taattgatct ccaaagcttt cccggaaata
     2341 gaattgaacc aggggagaat taattccact aacaatccag aaatgattac cgaaacacct
     2401 gccaacagca gggattcaga aataaactga atcgcaagct gattccgggt ggaacctaaa
     2461 gattttctaa caccgatctc tttagcgcgt tcggtggatt tagaagtggt aagattcata
     2521 aaatttacac cggcgattaa gagaataatt atcgccgcgg aagccataat gtagatataa
     2581 gaaatattgc cattgggttc cagttcccac cggatttgtg atttcaaatg aatatctgtg
     2641 agtggctgta aaataaatct taaattattg gtacgcaaat actccaacct ttcctcagga
     2701 atgtcaatat gttgcgccgc ccaatccatt aatttagctt cgagtgcttt tgcatctgca
     2761 cccggacgta attttatata attgtagtgt ccaaaatcat cccaggtata gtataaatct
     2821 ccaggccttt ttaaggatac ataacttatc aggccgtcaa aatgaaaatg ggaattaacc
     2881 ggaacatctt caatgacgcc cccaacttcc agggtatgag cccgctcatc atcagtaacc
     2941 tgcaggaatt ttcccacggg atcttcttcc ccaaagtatt tctttgcaga tttctcagta
     3001 attaccaaat atccgggctc cttcagaact ttgtcagcat ttcctttgag cagtttaaaa
     3061 ctgaatacct tcaggaaagt actgtcaaca cttaaaattc cttcttcatc aaaatgaaca
     3121 ttatttaccg gattgcgcac tgcaaatgtt gctcttgtta atcctggagc ccacagcgga
     3181 gaaagacttg tccctgcaat tacctccgga aattcttcaa ccatggccaa tggcatggga
     3241 tgaggcgtgc gggtttgagg atcatccgac tgccaggcga ttctgtaaat gtccccggcg
     3301 tttgcatgaa atttatcaaa actcaattca tcatttataa ataaaatgat gaggaatacg
     3361 cttgccattc ccatggaaag tcccgcgata tttatta
//
LOCUS       DASWHX010000167         8499 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_1282020, whole genome shotgun sequence.
ACCESSION   DASWHX010000167 DASWHX010000000
VERSION     DASWHX010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 8499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8499
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1282020"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            102..1235
                     /locus_tag="VGA21_11135"
     CDS             102..1235
                     /locus_tag="VGA21_11135"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HEX9651107.1"
                     /translation="MFSIIYGYRNRETERVLRSINSLVKSIPDFEYEIILIDYGSDID
                     ISNQLRIALGSIPNLQYSYADFRDRPWSRSHAMNIGFRLAKGKYMFTADIDLIFIPNF
                     WSIINSVKKENEVQFFPFYLLPRFFKYENERELGKNFITITEQGRGISLIPSSVLKMT
                     GGYDERFSYWGYEDNEFHHRCEANGINTIFLSDIPIAHHQWHKSAFEDSDFFPKGWRI
                     IMKTYFEQINFENRKFDLSKQGDLTNNVGWDSNEGLTETTITLDRAESFASLFTKIDF
                     LYTRLAEAGGIKITISLEDFEKFQKATLTRFVNLLNRVANTCKMKHLKVVSKYRAGHM
                     SYNEIRDVCSSFVFIKQVPISDYGLTLSKIEKKVVVKLYKSSR"
     gene            1232..2341
                     /locus_tag="VGA21_11140"
     CDS             1232..2341
                     /locus_tag="VGA21_11140"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX9651108.1"
                     /translation="MKRVFHLMPALNYGGTETVVASLCRYQINTLGYDSRIILFGENN
                     RMDISSDIQVIQILNCNASLGWLGNKMVGVEKLIELIKVEKPDVIHSHSEWTTLIALS
                     CKIKSVKYIFHYHRYFSKPKVRRSALTRFSENVLVTFLLTRFGNSKIIAVSEKLKGQI
                     FHLYPILRLYKNMVQVVGNPIPSARNGKPVPLAVKNPFLILTVSRLEHEKNIFAIISI
                     AEKLESLMPFIWEIYGSGNQSELFDKKVQAANLKNRIILKGVTNDLTNVYPKYDVYVS
                     VSNCETFGLTILESLSQGIPVIARKNGGSNDFLVNKLNAIYLDTDNPNKFAKAIHDIS
                     FDEKVKSIRLNGLKTASRYFINSIGQKIDLIYKSG"
     gene            complement(2415..3902)
                     /locus_tag="VGA21_11145"
     CDS             complement(2415..3902)
                     /locus_tag="VGA21_11145"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HEX9651109.1"
                     /translation="MENLPGQRVDLPVFSLQNGVIRDCSKSALEFLEVSKSDQVVNRE
                     ISKFFPYKQCNGDLSKEMWETRSYESVSSGIQSFPWKFITSQDKEIATVVSLVTYNDF
                     LLAYVNPFGNTNEEWDKFKDQQSLLLNINQKIEEGIYRSSLNNGFYYVNKSFVKMFGF
                     ASEDEVLQLKPEDLNALYDMTNTRSDVLRQIENHGNIRNKEVLFRKKDGSRFWGLESC
                     YLHLDEAGRKFIDGIIVDITPIKESENLLREKNHELQKVNEEMDRFVYSVSHDLRAPL
                     SSVLGLLNLAEMETDPAQLSLFIQMMKKSVKKLDHFINEILDYSRNARLKLEDDRVDL
                     ETLTTEIIKSIEYMDGFERIRKSVKVYSRSEFVSDKKRIKIVLTNLITNAVLYHDSKK
                     QNQFIEINAFIENESARIEVIDNGRGIQTTHLDKIFKMFYRASEDSKGSGLGLFMVKE
                     CVEKLEGTLNVSSTYGIGTKFEIVLKNFLANTARQSRLFGTSASA"
     gene            complement(4214..6613)
                     /locus_tag="VGA21_11150"
     CDS             complement(4214..6613)
                     /locus_tag="VGA21_11150"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010608161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HEX9651110.1"
                     /translation="MYVLKRDGRRESVKFDKITARIEKLCYGLDPRYVNVIDIAKKVI
                     EGIYDGVTTIELDNLAAEIAASLTTKHPDFAKLSARIAISNLHKVTSKSFSSTMKRLY
                     TYIDPKTGHNASLISKEVYGIVKKNAAQLDSSIIYDRDFNYDYFGFKTLERSYLMKLD
                     GRIVERPQHMLMRVAVGIHMEDIEAAIETYNLLSEKWFTHATPTLFNAGTPKPQLSSC
                     FLLTMKDDSIDGIYDTLKQCAKISQSAGGIGLSIHNIRAKGSYIKGTGGVSNGIIPML
                     RNFDMTARYVDQGGGKRKGSFAMYLEPWHADIFDFLELKKNHGKEELRARDLFYALWI
                     SDLFMKRVENNEMWSLFCPNEAPGLADCHSDEFEKLYTKYEAEGKYRKQVKAQDLWFE
                     ILEAQIETGTPYILYKDACNRKSNQKNLGTIKSSNLCTEIIEYTSPDEIAVCNLASIA
                     LPMFVTEDGIFDHQKLFEVTIVATKNLNKVIDVNYYPVEEARRSNMRHRPIGLGVQGL
                     ADAFIKLKMPFDSEEARGLNKDIFETIYYAALTASKDLAKKHGPYETFKGSPVSKGIL
                     QFDMWGVTPSSGRWNWDELKQEIKKHGVRNSLLLAPMPTASTSQILGNNECFEPYTSN
                     IYTRRTLSGEFIVVNKHLMKDLIELSLWNESMKNRLIEANGSIQNVNEIPGNIKELYK
                     TVWEISQKAIIDMAADRGAYIDQSQSLNIHIADPNFGKLTSMHFYAWKKGLKTGMYYL
                     RSKAATDAIKFTVDKEALKQIQPQQVMVVQEAMAMTREQKEADIACSLDNPDDCITCG
                     S"
     gene            complement(6657..7637)
                     /locus_tag="VGA21_11155"
     CDS             complement(6657..7637)
                     /locus_tag="VGA21_11155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009195238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase small
                     subunit"
                     /protein_id="HEX9651111.1"
                     /translation="MNSSSHEPILKENKDRFVLFPITHSDIWEFYKKAEASFWTAEEI
                     DLGQDLNDWEKMNEGERHFISHVLAFFAASDGIVNENLAENFVSEVQYTEAKFFYGFQ
                     IAIENIHSETYSLLIDTYIKDPREKDRLFHAIETMDCVKKKAEWALKWIENASFQERL
                     IAFAAVEGIFFSGSFCSIFWLKKRGLMPGLSFSNELISRDEGLHCDFACLLYNNHVQN
                     KLSVKRVTEIIENAVEIEKEFVTDAIPVKLIGMNADMMCQYIEFVADRLLLALGCKKI
                     YNSTNPFDFMEMISLQGKTNFFEKRVGEYQKAGVMKNTELSPKFSLDEEF"
     gene            8153..8461
                     /gene="rplU"
                     /locus_tag="VGA21_11160"
     CDS             8153..8461
                     /gene="rplU"
                     /locus_tag="VGA21_11160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018475241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEX9651112.1"
                     /translation="MYAVVDIAGKQFKVSQDMKVYAPLIDAEKGKKVDFDKVLLVDRN
                     GKIEVGSPYVKGAKVSAEVLDHLQDDKVIIFKKKRRKGYKLKNGHRQRLTQLLIKKIS
                     "
BASE COUNT         2604 a         1623 c         1541 g         2731 t
ORIGIN      
        1 cataagacat acaaaggaat tcaattacct ctgaagaaaa ggttaaagtt attcgcacga
       61 aaaattatct gcataatgcg ttatctcttt caggtatggt aatgttttcc attatttatg
      121 ggtacaggaa tagagaaaca gaaagagttt taagatctat taattcttta gtgaaatcga
      181 tacctgattt tgaatatgaa attattctta tagattacgg cagtgacatt gatatttcta
      241 atcaattaag aatcgcttta ggttcgattc ctaacctgca gtactcatat gcggatttcc
      301 gggatagacc ctggagtcga tctcatgcca tgaatattgg atttagattg gcaaaaggta
      361 agtacatgtt tactgctgat atcgatctaa tattcatccc gaacttttgg agtattatta
      421 atagtgttaa gaaagagaac gaagtccaat tctttccctt ttacctgttg cccaggtttt
      481 ttaaatatga aaatgaaaga gaattaggta agaattttat tactataacc gaacagggaa
      541 gaggaatttc cctcatccct tcgtctgtac taaaaatgac aggtggttat gatgaaagat
      601 tttcctattg gggatatgaa gataatgaat tccaccaccg atgcgaggca aatggaatta
      661 acaccatatt tctgagtgat atcccaattg ctcatcacca gtggcataaa tcagcctttg
      721 aggattctga cttttttcct aaaggttgga gaataataat gaaaacttat tttgagcaga
      781 taaatttcga aaataggaaa tttgatttaa gcaagcaggg agatttaaca aataatgttg
      841 gttgggattc caatgaaggg ttgactgaaa caacaatcac tcttgacaga gcggagtcat
      901 ttgcttcact gttcacgaaa atagattttt tatatacgcg gcttgcggaa gccggtggta
      961 taaaaataac tatatccctg gaagattttg aaaagtttca aaaagctaca ctgacgcgct
     1021 tcgtcaattt gttgaatcgg gtggctaata cctgtaagat gaaacatctt aaggtagtta
     1081 gtaaatacag agccggccat atgtcttata atgagatcag ggatgtttgt tcctcctttg
     1141 ttttcataaa acaagtgccg attagtgatt atgggttaac cttaagcaaa attgaaaaaa
     1201 aggttgtagt caaattgtat aaatcttcgc gatgaaaagg gtattccacc tgatgcccgc
     1261 tttaaattac ggagggacgg aaactgttgt agcctccctt tgcagatatc agataaatac
     1321 ccttggctat gattcccgga ttattctttt tggagaaaat aaccgaatgg atatctcttc
     1381 tgacattcaa gttatacaaa ttttgaattg taatgcctcg cttggctggc taggcaataa
     1441 aatggttggg gttgaaaaac tcatagaatt aattaaggtt gaaaaacctg atgtaattca
     1501 cagtcattcc gaatggacaa cattgatcgc gctttcttgc aaaataaaat ctgtaaaata
     1561 tatattccat tatcaccggt atttctcaaa accaaaagtt agaaggtcgg cattaacccg
     1621 attttctgag aatgtattag tgacgtttct tctcaccagg tttgggaatt cgaagataat
     1681 tgctgtctct gaaaaactta aaggtcaaat atttcattta tatccgattc ttagactgta
     1741 taaaaatatg gttcaggttg ttgggaatcc aattccttca gcaagaaatg gaaagcctgt
     1801 tcctttagca gtcaaaaatc ctttcctaat tctaacggtc tccaggcttg agcatgagaa
     1861 aaatattttt gctattatat ccatcgctga aaaattggaa tccctgatgc cgtttatctg
     1921 ggaaatctac ggaagtggaa atcaatctga attatttgat aaaaaagttc aggcggctaa
     1981 tttaaagaat agaattattt tgaaaggagt gacaaatgat ctcacaaatg tataccctaa
     2041 gtatgatgtt tatgtaagtg tatcaaattg tgaaactttt ggacttacta tcctcgaatc
     2101 attatcacaa ggtattccgg taatagcgag gaaaaatggc ggttctaatg atttcctggt
     2161 aaacaagttg aacgctatat acctggatac cgataatccg aataagtttg ccaaagcaat
     2221 tcatgatatt agttttgatg aaaaggtaaa atcgataaga ttgaatggat taaaaaccgc
     2281 atcgcgatat tttattaact ctattggtca aaaaattgac ttaatttaca aatcaggttg
     2341 agattgccat gcaagtcatc caacctcaaa gtgaaggata ggtggattct aaatggggat
     2401 tgcccttata agtcttatgc agaagcactc gtcccgaaaa gcctggattg gcgcgcggta
     2461 tttgcaagaa agtttttcaa cacaatctca aactttgttc caatgccata ggtcgaagaa
     2521 acgttcaaag ttccttccaa tttctcaaca cattccttca ccatgaatag gccgagacca
     2581 gaaccttttg agtcttcaga tgccctgtaa aacattttaa atattttgtc cagatgagtt
     2641 gtctggattc cccttccatt atcaattact tcaattcttg cactttcgtt ttcaataaat
     2701 gcattgatct caataaattg attctgcttt ttcgaatcat gatacaaaac cgcgttggta
     2761 ataagattag tgagtacaat tttgatcctt tttttgtccg aaacaaattc gctgcgggaa
     2821 tatactttta ctgattttcg aatccgctca aaaccatcca tgtattcaat gctcttgatt
     2881 atttcagtgg ttaatgtctc aagatcaact ctgtcatctt ccaatttcaa tcttgcattg
     2941 cgcgaataat ccagaatttc attgatgaag tgatccagtt tcttcacact tttcttcatc
     3001 atctgaatga acaaggaaag ctgggcagga tcagtttcca tttctgccag gttcagcaaa
     3061 cccaatactg atgacaaagg agcacgtaaa tcatgggaaa cactataaac aaagcgatcc
     3121 atttcttcat tcactttttg aagttcatgg ttcttttccc tcagtaggtt ttccgactct
     3181 ttaatgggtg ttatatccac aattattccg tcaatgaatt tcctgccggc ttcatccaaa
     3241 tgtaaataac aactttccag accccagaat cttgatccat ctttctttct aaacagaact
     3301 tccttattcc gaatattacc gtgattctca atctgccgca gcacatctga gcgagtgtta
     3361 gtcatatcat aaagagcatt gaggtcttcc ggctttagtt gcaggacttc atcctcactg
     3421 gcgaaaccaa acatctttac aaacgattta tttacataat aaaatccatt gtttaatgag
     3481 ctgcgataaa taccctcttc aattttttga tttatgttta atagcaggct ttgttggtcc
     3541 ttaaatttat cccactcttc attggtattt ccaaatggat taacataggc tagcaagaag
     3601 tcattgtaag taacaaggct gaccacagtt gctatttcct tatcctgact agtaatgaat
     3661 ttccagggaa aggattgtat tccactgctg acagattcgt aggatcttgt ttcccacatt
     3721 tctttggata aatctccatt gcattgtttg taaggaaaga acttgctgat ttccctatta
     3781 acaacttgat cacttttgct gacttccaga aattccaggg ccgattttga gcaatccctg
     3841 attactccat tttgcagtga aaatacaggc aggtctaccc tttggccggg taggttttcc
     3901 attattaggt aatgaataac aatagtaaac taagcataat tatagtcata cttcacataa
     3961 taatagcaat aattctggaa ataaacctac tgaatgtaat atccggacgg tatttaacaa
     4021 taaattcctt taaattatta cgtattcaca acccggaaat aacatctcta tttaaaggcc
     4081 tcgaacgagt tcttaaataa gtggcgccaa ttaaattccg acttcaacct ctttagcaga
     4141 aaaattcatt tcaggtttgc aatgaaaaaa gagtcaaaag gacacctttt ttggatgttt
     4201 ccatgagtga aatctagctg ccgcaggtaa tgcaatcatc cggattatcc agcgaacagg
     4261 caatatccgc ttctttttgc tcccgggtca ttgccatagc ctcctgcaca accattacct
     4321 gttggggctg tatttgtttt aaggcttctt tatctacggt aaatttaatt gcatcggtag
     4381 cagccttaga tctcagataa tacatgcctg tcttcagtcc ttttttccat gcgtagaagt
     4441 gcatggaggt aagtttgcca aagtttggat ccgcaatatg aatattcaga ctttgacttt
     4501 gatcaatata agcaccacgg tcagccgcca tatcgatgat tgctttttga gaaatctccc
     4561 aaaccgtctt atacaattct ttaatattcc cgggaatctc gttaacattc tgaatggatc
     4621 cattggcttc aatcaatctg ttcttcatgg actcattcca caggctcaat tcgatcagat
     4681 ctttcattag gtgtttattc accacaatga attctcctga caaagtgcgg cgggtataaa
     4741 tgttagatgt ataaggttcg aaacattcgt tattacccag gatttgtgag gttgaagcgg
     4801 ttggcattgg agctaacaaa agagaattcc ggacaccgtg ttttttgatt tcctgcttca
     4861 gttcatccca gttccaacgc cctgaagatg gtgtaacacc ccacatatca aattgtaaga
     4921 tacccttgga aacaggcgac cctttaaacg tttcataagg gccatgtttt ttggcaagat
     4981 cttttgatgc ggttaacgct gcataataaa tggtttcaaa gatatccttg tttaatccac
     5041 gggcttcttc tgaatcgaag ggcatcttta gttttatgaa ggcatcggca agcccctgaa
     5101 caccgagacc aatcggacga tgtctcatat tcgatcttct tgcttcttct acaggatagt
     5161 aattgacatc gattaccttg tttaggtttt tagttgctac aatagtaact tcaaataatt
     5221 tctgatgatc aaatatccca tcttcagtga cgaacatggg caaggcaatt gatgccaggt
     5281 tacaaactgc tatttcgtca ggagaagtat attcaattat ctccgtacat aaattgctgg
     5341 attttattgt tcccaggttt ttttggtttg atttccggtt acatgcatcc ttatatagaa
     5401 tgtatggtgt tccggtttca atttgagctt ccagaatttc gaaccagaga tcctgagctt
     5461 ttacctgctt tcggtatttt ccttcagcct catatttggt atacaacttt tcaaattcat
     5521 cactgtgaca gtcagccaat ccgggtgctt cattcggaca gaataacgac cacatttcat
     5581 tattttcaac acgcttcatg aaaaggtctg aaatccataa agcgtaaaat aaatccctgg
     5641 ccctcagttc ttcttttcca tgatttttct ttagttccag aaaatcaaaa atatctgcgt
     5701 gccacggttc cagatacatg gcaaaactgc cttttctttt gccaccaccc tgatcaacat
     5761 agcgcgcggt catatcaaaa ttccgaagca taggtataat accattgctg acgccaccgg
     5821 ttccttttat gtaagatcct ttagctctga tattgtgtat gcttaacccg ataccgccgg
     5881 cggattgtga aatcttggca cactgtttca gcgtatcata gataccatca attgaatcat
     5941 ccttcatggt taacaggaag catgaagaaa gctgaggctt gggtgtacca gcattgaata
     6001 aagttggtgt agcatgtgta aaccactttt ctgaaagaag attatacgtt tcgattgccg
     6061 cctcaatatc ttccatatga attcctacag caacacgcat cagcatgtgc tgaggccttt
     6121 ccacaatcct gccatccagt ttcattaggt acgatctctc cagagtttta aatccgaaat
     6181 aatcgtaatt aaaatctctg tcatagatta tcgatgaatc aagttgagcg gcattcttct
     6241 taactattcc atatacttct ttggaaatca aagaggcgtt atgaccggtt tttggatcaa
     6301 tgtaagtata gagtcttttc atggtgcttg aaaaagactt actcgtcacc ttatgcaaat
     6361 tagaaattgc aatccttgca gatagttttg caaagtcggg atgtttggta gttaaagaag
     6421 ctgcaatttc agcggccagg ttatcgagct caatggtggt tacaccatca taaatacctt
     6481 cgattacctt cttagcaata tcaatcacat tcacgtatcg aggatccaga ccatagcaaa
     6541 gtttttcgat cctggcagta atcttgtcaa atttgactga ttctctgcga ccgtctctct
     6601 ttaaaacata cattgcataa ttagattagt gggtttacaa taaatttatc aggtaattaa
     6661 aattcttcat caaggctaaa cttaggagat agttcggtat ttttcattac acctgctttc
     6721 tggtattctc ctactctttt ttcaaagaaa ttagtttttc cctgcaaaga aatcatctcc
     6781 ataaaatcaa acggatttgt ggagttgtat atcttcttac agcctaatgc taacaaaagt
     6841 ctgtcagcaa caaattcaat atactggcac atcatatccg cgttcatccc aattagtttt
     6901 accgggattg catcggttac aaattctttt tcaatttcaa ctgcattctc aataatttcc
     6961 gtaacccgct ttacggataa tttattctga acatgattat tgtaaagcaa gcaggcaaag
     7021 tcgcaatgta atccttcatc acgggaaatt agttcattgg aaaaacttaa gcctggcatc
     7081 agacctcttt tcttaagcca gaaaatggaa cagaaacttc cggaaaagaa aattccctcc
     7141 actgcggcaa aagcgataag tctttcctga aaggatgcgt tctcaatcca cttcaatgcc
     7201 cattccgctt tcttctttac acaatccatc gtctcgatag cgtgaaaaag tctgtccttc
     7261 tcccggggat cttttatata agtatcaatc agcagggagt acgtttctga atgaatattc
     7321 tcaatagcga tttggaaacc ataaaagaac ttggcttccg tgtattgcac ctctgaaacg
     7381 aagttctcag ccaggttttc atttactatt ccatccgatg cagcaaagaa agcgagcacg
     7441 tgggagatga agtgtctctc accttcattc attttttccc aatcatttaa atcctgacca
     7501 aggtcaattt cttctgctgt ccagaaacta gcttcagcct ttttataaaa ttcccaaata
     7561 tcactgtgtg ttattgggaa taaaacaaat cggtccttat tttcttttaa gatgggttca
     7621 tggctgctgc tattcatttt atctcctttt atataattaa aaataaaaaa tctcttctaa
     7681 tgattgttta aacgcccgtg ttgtcgcttt aaggttagaa aaatgcaaaa gtcgttacac
     7741 aatcatttct gactaatttt aaaaaagtct gttagtttta agttgatttg cctccgaaaa
     7801 cctttttaaa ttttcgaatg cgaagaaggg ttatcgcctt aaaataacct ctacaaatat
     7861 tgcaaattga tggggagaaa tcaaagaaaa aatggccctg gtaggcttcc agaagtggtt
     7921 ttatccatat tgataaaatc caatttaagt aattgtaatt aagaccatta ttaaagaatt
     7981 cttaaggtat acttccatca aatcagcgta aaataggctg tatttttttt tcaaaaatgt
     8041 caaaaaaaaa ttcaaattaa gcctttagtt cttttagtag gccttttagt ttgtaattga
     8101 aggattagaa tctatatttg caatcctttt tgaataatgg atttataacg atatgtacgc
     8161 tgtagtagac attgcaggaa agcagtttaa ggtgagccag gatatgaaag tctatgcccc
     8221 tttaattgat gccgaaaaag gcaagaaagt ggactttgac aaggtattgc tggttgacag
     8281 gaatggaaag attgaggtgg gttcacctta tgtgaaaggg gcaaaagttt ccgcagaggt
     8341 tcttgatcat ttacaggacg ataaggttat catctttaag aaaaagagaa gaaaagggta
     8401 taaacttaag aacggccaca ggcagcgctt aacccaactg ttaattaaga aaatttcgta
     8461 agaaatggcg cataaaaaag gagcaggtag ttccagaaa
//
LOCUS       DASWHX010000168         3720 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_3492613, whole genome shotgun sequence.
ACCESSION   DASWHX010000168 DASWHX010000000
VERSION     DASWHX010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 3720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3720
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3492613"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..964
                     /locus_tag="VGA21_11165"
     CDS             <1..964
                     /locus_tag="VGA21_11165"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9651113.1"
                     /translation="PVKPDSVKGRFKFMKRIYYRILNAIPFIGRKKEQFKALSGVSLS
                     IGHGMFGLLGPNGAGKTTLMRILCGIIDQSYGKIWINGQDTKLKREELQGLIGYLPQS
                     FGTYENMTPYQFLDYQAILRKITNRKEREDRIQYVLKAVHMDEHKDTKIGSFSGGMKQ
                     RIGIAQILLHLPKILVVDEPTAGLDPLERIRFRNLLVELSRERIVVFSTHIIEDIASS
                     CNNLAVIIKGNIEYKGSPASMAELAKDKVWEIVMPADKFEQVRDNYLIVHHMREGDTI
                     RIKCLSKSKPGETAKPVRANLEDAYLWLIKSKTNNHGELSDITD"
     gene            936..1640
                     /locus_tag="VGA21_11170"
     CDS             936..1640
                     /locus_tag="VGA21_11170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013453214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651114.1"
                     /translation="MVSYLTLLIRIVNYNIKVIFANKFIYFALAALLFFGFIITITIF
                     EDPNFNEAVIYGFLVFPGLLLIFYPMAFGIQNDDDSNMLETIFGIPNYRYKVWLIRFV
                     ITILVAFVILYLLGVIADFTMYRFNILPMIGQVLFPVTFLASIAFMVSTLIKNGNGTA
                     IVIIIIALIFFIFAEELEYNAWNVFLNPFSEPRDMSEFVWLSIISKNRIYLTVGTSLC
                     ILYGLFNLQFREKFMQ"
     gene            2033..3064
                     /locus_tag="VGA21_11175"
     CDS             2033..3064
                     /locus_tag="VGA21_11175"
                     /inference="COORDINATES: protein motif:HMM:NF040322.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651115.1"
                     /translation="MRIIERHGILILLINLIPKYFRPMTDHYITWWNVENLFDIKDAP
                     ESRRPPDIARKIKADLTNWTQDVLDRKIQNLASVIMQINNGLGPDLLGVCEIENAHVL
                     NLLVQAVNLGARNYQLIHHDTLDNRGIDVAFIYDANKYQFNAQEFFHHEIVKRYPTRE
                     IVQATIATQKGNEIILVGNHWPSRSGGQYESEPYRIIAGETLSYFIERIQQVKGNDAA
                     ILVMGDFNDEPFNRSLMDYAQSVRDRDKVVNGRNPYLLNLMWPVLGERNASYVFDGRP
                     IMLDQFLVSKGIVKKTGKFDLSDNAKLEKFAGMVRGDYDIPVRFGQETPNLNGFSDHL
                     PISFVLQEK"
     gene            complement(3151..>3720)
                     /locus_tag="VGA21_11180"
     CDS             complement(3151..>3720)
                     /locus_tag="VGA21_11180"
                     /inference="COORDINATES: protein motif:HMM:NF023295.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3387 domain-containing protein"
                     /protein_id="HEX9651116.1"
                     /translation="EILSDEFLLEVQGMKHKSLAIELLKKILNNELKTRAKTNLVKSR
                     KLLQMLETAIKKYQNNLLTTAEIIQELIRIAKAIKEADKEGEKLGLNIDEVAFYNALE
                     VNDSAVQVLGDDTLKEIAREIADKVRANATIDWTIRESARAKLMVLVKRTLSKYGYPP
                     DKQQKAIDTVLKQAELLADGLVEGRSNPY"
BASE COUNT         1091 a          731 c          780 g         1118 t
ORIGIN      
        1 acctgtaaaa ccagactcgg ttaaaggtag attcaaattc atgaagcgga tttattaccg
       61 gatactgaat gcaattccat tcattggccg taagaaagaa cagtttaagg cactttcagg
      121 agtgagttta tctattggtc acggaatgtt cgggttgttg ggcccaaatg gcgctggcaa
      181 aaccaccttg atgcggatcc tgtgtggaat catcgatcag agctacggta aaatctggat
      241 caacggccag gataccaaat tgaagcggga agaactgcag ggacttatcg gttatctgcc
      301 ccaatcattt ggtacttatg aaaacatgac tccttatcag ttcctcgact accaggccat
      361 tttaagaaaa attactaata gaaaagagag ggaagaccgg attcaatatg tattaaaggc
      421 agttcatatg gatgaacaca aggacactaa gattggatct ttttccggtg gaatgaaaca
      481 gcggattggg atcgcgcaga ttttattgca ccttccaaaa atcctggtag tggatgagcc
      541 aactgccgga ctggatcctc tggaaagaat ccgattcagg aatctattgg tagaacttag
      601 tcgtgaaaga attgtcgtgt tctcaacgca cataattgaa gatattgcca gcagctgcaa
      661 caaccttgct gtaattataa agggaaacat tgaatataag ggaagtcctg ccagtatggc
      721 tgaacttgca aaagacaagg tttgggaaat tgttatgccc gcggataagt ttgaacaggt
      781 tagggataat tacctgattg ttcatcatat gcgtgaaggt gataccatac gaattaaatg
      841 tctttccaaa tccaaacctg gtgaaacagc aaaaccggtt agggccaatc ttgaagatgc
      901 gtacttatgg ttaattaaaa gtaaaacaaa taatcatggt gagttatctg acattactga
      961 ttaggattgt caattacaat atcaaagtaa ttttcgccaa caagtttata tactttgctc
     1021 tggcagcatt gttattcttt ggtttcataa taacgatcac catctttgaa gatccaaact
     1081 tcaatgaagc cgtgatttat ggctttcttg ttttcccggg actcttgttg attttctacc
     1141 cgatggcctt tggaatccag aatgatgatg attcaaatat gctggaaacc atttttggaa
     1201 ttcccaatta ccggtacaag gtgtggctaa taaggtttgt cattactatc ctggttgcct
     1261 ttgtaattct ctacctcctc ggagtgattg ccgacttcac tatgtacaga tttaacatct
     1321 taccaatgat aggacaagta ttgtttccgg taacatttct ggcttctatt gccttcatgg
     1381 tttcaacatt aatcaagaat ggcaacggta ccgcaattgt tataatcata attgccttga
     1441 tcttcttcat tttcgcggaa gagttggaat ataatgcatg gaatgtattt ctgaatcctt
     1501 tcagcgaacc ccgcgacatg agtgaattcg tatggcttag catcatctca aaaaacagga
     1561 tatacttaac tgtgggaaca agtctctgca ttctttatgg cctgttcaac ctgcagttca
     1621 gggaaaaatt catgcaataa ttttctgaaa ttattctctg aagctggtgc gttttcccct
     1681 ccttccgaaa caacttatta aatgaaaaca gactgcaaaa ggaggggtag gggtggtttt
     1741 gctaacattc aatctccggg actgttttct tcacgggggg tccctttcgg agaccccacg
     1801 gtgacaggca aatcccatgg atcaactttc ctgggatttt ttatgagcta tttcgcctgc
     1861 atattgggta actggcaata ccctgccgaa acaataaaat agttaacctc aaatggagag
     1921 aggtactgaa caggtatagc tgtttctact gtctgcaatt gtttgcatct tgggcacaag
     1981 gaacaagcca tagatcaact cctaaatctt acatctgtaa taaacacaat caatgagaat
     2041 cattgaaaga catggtattt taatcctatt aattaatttg attccaaaat attttagacc
     2101 tatgactgat cattatataa cctggtggaa tgtagaaaac ctgtttgata taaaagatgc
     2161 tcctgaatca agaaggccac ccgatatagc gaggaaaata aaagccgatc taaccaattg
     2221 gactcaagat gtattagatc gaaagattca gaatcttgcc tcggtgatca tgcagattaa
     2281 taatggactg gggcctgatc tcctcggagt ttgtgagata gaaaatgccc atgtattaaa
     2341 tcttttagtc caggcagtga atttaggagc aaggaattac cagctgatac atcacgatac
     2401 gcttgataat cggggaattg atgtggcgtt catatatgat gccaacaaat atcagttcaa
     2461 tgcccaggag ttctttcatc atgagatagt gaagcgatat cccacccgtg aaattgttca
     2521 agctacgata gcaacccaaa agggaaatga aataattctg gttgggaatc attggccctc
     2581 acgctcggga ggacagtatg aatctgaacc atacaggata atcgccggtg aaacccttag
     2641 ctacttcatc gaaagaattc agcaagtaaa agggaatgat gcggccatcc tggtgatggg
     2701 tgattttaat gatgaaccct ttaaccgatc attaatggat tatgcccaaa gcgttcgtga
     2761 ccgggataaa gtagttaatg gcagaaaccc ttatttactg aacttaatgt ggccggtatt
     2821 gggagaaaga aatgccagtt acgtatttga cggaaggccc attatgcttg accaattcct
     2881 ggtgtctaaa ggcatagtga aaaaaaccgg aaaatttgat ttaagtgata atgcaaaact
     2941 ggaaaaattt gcaggaatgg taagaggaga ctatgatatt cctgtgcggt tcgggcagga
     3001 gacaccaaat ttgaatggct ttagcgacca tttgcctatc tcttttgttc ttcaggagaa
     3061 gtgaacacta tctaaatgca taaataagtt tgcccttgga tcacagcttc attgagaata
     3121 atgtcaggtg catctatttc acctatgtta ttaatacggg ttactcctgc cctccaccaa
     3181 cccatccgcc aacaactccg cttgtttcag caccgtatca atagccttct gttgcttatc
     3241 tggaggatag ccgtattttg aaagcgtccg ttttaccaat accatcaatt tagcccgtgc
     3301 gctttcccgg atggtccagt ctatcgttgc attggctctt actttgtctg caatttctct
     3361 ggctatctct ttgagtgtgt cgtctcctaa tacttgaacg gcgctatcgt tgacttccag
     3421 tgcattgtaa aaagcgactt catcaatatt aagcccaagt ttttctccct ctttgtcagc
     3481 ttctttgatc gctttggcta tgcgtatcag ttcctggatg atttcggctg tggtcaataa
     3541 attgttctga tatttcttga tggctgtttc cagcatctgc aatagtttcc ggctctttac
     3601 aagatttgtt tttgcacggg ttttcaattc attgttcaaa atctttttca acaattcaat
     3661 tgcaaggctc ttatgtttca tgccctggac ttccaaaaga aattcatcgg ataaaatttc
//
LOCUS       DASWHX010000169        28196 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_917833, whole genome shotgun sequence.
ACCESSION   DASWHX010000169 DASWHX010000000
VERSION     DASWHX010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 28196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28196
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_917833"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..286)
                     /locus_tag="VGA21_11185"
     CDS             complement(<1..286)
                     /locus_tag="VGA21_11185"
                     /EC_number="2.3.1.275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009581727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HEX9651117.1"
                     /translation="MEIFLLTAGILIAYLLGAVPTAVWFGKLYHGIDVREHGSGNPGA
                     TNTFRVLGKRAGTIVLLIDVVKGLMAASLPVVLLKLQTFPENELVNFKIIF"
     gene            complement(339..5252)
                     /locus_tag="VGA21_11190"
     CDS             complement(339..5252)
                     /locus_tag="VGA21_11190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651118.1"
                     /translation="MHNGKLFALLNPKICVIFVYFNKSKPENLPIKKIISSKVIIGLL
                     FWVGGIFSVNTTLAQRSSSFPDEPATFLAELQNRLENSNQDVLVALASDVGRIWKADL
                     GLDLQTKLIAHFKIMSEKGYKLNGRLSEYLETLVMAIDQEGLNQTKLLSYMNMTEEVI
                     KSFESRDLMTYFNSMRNFFEFRALYYSNFSNLIVTNDEYTFEYVGSVTILDLEIEEEA
                     EIQKKEVNTATGAFESWDSEPAEEEWGSEWGNEWGDEKSGEEEPVQTLEDVLIQDAVQ
                     PPVSGPTIVFSKLDLVFTTQIDSALTLSKTAGSFTIKDGMFIGEGGKFDWSRTGLGDT
                     VFVTLSKYNFDTKRPVIEAENVTLTYSGKVAEPIEGIFSFNGLVTNKTSYPRFISYRN
                     NIPVVGIGNEDLSYTGGFSLNGKRIVSSSIFEGLSKIEVTKNGVKKFKARSRLFELGD
                     SLITAERAAIVIYHERDSITHPTVRVSYNTKTDDLILQKDKGGFKRTPYSASFFNTDF
                     IADIVRWEVNSDSLEISILNARDEIPAVFESKEYYNPDQVTGSIALYKFNPLSMVMGY
                     YNKKGSPIYLESLEKFTGLERNTIKSSLFYLMWAGFIDYNINTQEVTIKEKALHTTDA
                     QKSKKDFDNIRMLSLTNTNSNATFDMANEQIVVRGIEKFYISEILDVYIQPDSSTITL
                     LENRTFKFDGKVFAGNFEYIGRDFTFNYDAFTLELNQIDSIEFYVLEEDSRGSRRRKV
                     DNAISGITPEDSIASEDGSTMAATSGTLFINRANNKSGRKIFPDYPKFDGTGSSSIVY
                     FNRPEYLGGVYNRSVYFLVPPFKLDSLSNSDPSSIGFTGTFGSSGMFPDFKESLKIQP
                     DFSLGFEHSLPPSGYPLYNTTARFFNKMKLDKAGIRGNGEIKYLTTDLISNDFIFYVD
                     SVTTEGSVAEVRKETHLGTSFPKLYVENYKLRWLPRKDSMYITNLKAPIQIYDNTATL
                     DGTANITKGGIKGSGTLLTRGSQSVSSRYAFNQNNFSARQADFEVKSSNPEKPVLKAD
                     GVKLNFDLTQNFAEITPEVAGDDVLEFPFAQFKTSIPSAVWDLDSSIVKMAKPAQFQL
                     EDSYFYTTRKDLDSLAFYATEAVYDIKKLELKVSGIPFITVADAKITPEGGEVLILEN
                     AQIGTLKNTTLVIDTLNEYHRLYDGTITIISRKKFEGEATYQFVNASEDTFAIKLTNF
                     RLEDLSANKRRQEFHTVANGGISQDDNLLISPGMYYKGDVKMIANSPALELDGYVKLD
                     LKKISGYDTWIRYQSAAEQQEVIFDFDNSVTENGLNVSAGLHFDVNDFSLYSTFVTDK
                     RNYDDEDFFRPGGNLKYLQDSAQFIIISPQKESGETLAGKSFTFNEETGDISFDGTLQ
                     FLENDKSRSITAAGVGTGNLETNEFDFNAFMSIIFNIPQSAFDLMAVDLKNSINLYGA
                     PEALGDKTTLLYKIANLFGDKVAKTYEEQSAMVYIPLVSISPAVVKPIVLSNVDLKWS
                     DINKAFYSVGGIGVSNILREDINGSFEGFLEIIKRLDGERIDLFLKASVDSWYYFSYE
                     NNKLITYSSNPAYNEFIATKSNAGKAKIGEFVFIPGDESEILTFVNRFRNDYLNIQQP
                     YQLNAAPIEEDLTDDGFGGKKKKDGNR"
     gene            5325..5723
                     /locus_tag="VGA21_11195"
     CDS             5325..5723
                     /locus_tag="VGA21_11195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017730176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HEX9651119.1"
                     /translation="MAKGRKKKEKTRFTEKELKEFETIINDKLDKARGEFKVLKETLN
                     RTNDSGTDNTAGTLKILEDGADTLEKESLSQLAARQQKFIAQLENALVRIMNGTYGVC
                     VDTGKLISKERLRAVPHTMHSIEAKLNRDR"
     gene            5796..6281
                     /gene="scpB"
                     /locus_tag="VGA21_11200"
     CDS             5796..6281
                     /gene="scpB"
                     /locus_tag="VGA21_11200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014771401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEX9651120.1"
                     /translation="MECLSEMFGADIPEEDITKALEDVKNKYEDEAFSLQLVHTGGGY
                     QFLTKPAYQSSISILLKHQSRKRLSTSALETLSIIAYKQPVSKPQIEQIRGVNCDYSI
                     QKLLDKGLIEIKGKADSVGKPIIYGTSEFFMDYFGINDLQELPTPKDFQAEQNTIGEE
                     K"
     gene            6314..7066
                     /locus_tag="VGA21_11205"
     CDS             6314..7066
                     /locus_tag="VGA21_11205"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009578606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEX9651121.1"
                     /translation="MKRKKSGNPSKSPPSELIRLNRFIANSGVCSRREADQLIERGLI
                     KVNKEVITQLGYKVRRGDAVFYKGKKLSPEKYVYVLLNKPKDYITTAKDPEGRKTVME
                     LVSNACSERILPVGRLDRNTTGLLLFTNHGELARKLSHPSNNVRKIYRVTVDKPISKS
                     DLDAIAVGVKLEDGLVEIDDLAILNTQKTELGLELHSGRNRIVRRLFESLGYDVVKLD
                     RAVYAGLTKKNLSRGRWRFLTEREVGMLNKGL"
     gene            complement(7183..7255)
                     /locus_tag="VGA21_11210"
     tRNA            complement(7183..7255)
                     /locus_tag="VGA21_11210"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7220..7222),aa:Phe,seq:gaa)
     gene            7358..8680
                     /locus_tag="VGA21_11215"
     CDS             7358..8680
                     /locus_tag="VGA21_11215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013454875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX9651122.1"
                     /translation="MLLVQQITFIIVLAVAIFFLRQRVLMIRRNILMGKNLDRSDRSS
                     ARWHTMLLVAFGQKKMFKNFIPAFLHLLIYLGFILINIEVLEIVLDGILGTHRLFAPF
                     LGDFYNFLINFFEFLALGVLVACIIFLIRRNVLKLKRFHSAEMTAWPRLDANLILIIE
                     CFLMVAILSMNAADQLLQQVHADGYTQTGSFIFSAALFPLFESLSQTGLLVLERFAWW
                     FHIIGILAFAIYITYSKHLHIFMAFPNTYYSNLESRGKMDIMPTVTNEVKLMLGLQSE
                     SSIPPPTEAERFGAKDVTDLYWKNLMDAYACTECGRCTAECPANLTGKKLSPRKIMMD
                     TRDRTEELGNHKSKNTTDSKSLLGDYITKEEINACTSCNACVEACPVNIDPLSIILQL
                     RRYVTMEESAAPASWNSMFQNIETNFAPWKFSPQDRFNWAEPLKEKKQ"
     gene            8677..9480
                     /locus_tag="VGA21_11220"
     CDS             8677..9480
                     /locus_tag="VGA21_11220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013454874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEX9651123.1"
                     /translation="MSDSAISYSVPTVAEMAAKREVPEILFWVGCSGSFDDRSKKITI
                     ALIKILNHAGIKFAVLGAEESCTGDPARRAGNEFLFQMQAMNNIRILNGYNIKKIVTA
                     CPHCFNTLKNEYPELGGNYEVVHHSQFIQQLIDEGKLKMKEDGEFKGSKITYHDSCFL
                     GRANNVYEAPRKVLEALDADLIEMKRCKTKGLCCGAGGAQMFKDAEPGKKEVNTERTE
                     EALETGAKVIASACPFCMTMLSDGVKTREKENEIKILDLAELISGNLNL"
     gene            9497..9997
                     /locus_tag="VGA21_11225"
     CDS             9497..9997
                     /locus_tag="VGA21_11225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651124.1"
                     /translation="MFVDFENLPGDAKIWIYAGNKRFTGNQREWMSEALQEFLEKWTS
                     HGNDLKSGFKVLYDQILVIGLDENAADASGCSIDASVHFIKEIGEHLEVNFFNRNLVP
                     FLHINNLTLVNLNSLKTKIANGEITGESTIFNTLLAKKSDLNQSVIPLKDSWLNRYLK
                     NEEIST"
     gene            10063..12123
                     /locus_tag="VGA21_11230"
     CDS             10063..12123
                     /locus_tag="VGA21_11230"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX9651125.1"
                     /translation="MKNLVKIILIAAFITGASLSFGQSKALKKGVKKITKGEYDLAIS
                     QFQKYTDSRELGGEANFYLGECYRLSNRVHQAEDYYKKAIERGYRAEEVYFYYAFALK
                     MNRKYKDAQIQLERFLNVAENKELIKHANVELDNLAKIDEILNKKTYYTVASLEDLNT
                     ENAEYSPVYQDGFLYFTSNRGVDKIYKATGTGFTDLFRVSTQGANVEIRSLENLGEEI
                     NLFNVNEGSVTFTPDGRTMVFARGNSGKRKGSENVDLFITRFRNREWSDARPININDN
                     GAWDSTPAFSANGRILYFASDRKGGYGGVDLYAATIDRTGRFTRVRNLGPEVNTPGNE
                     MFPYVADDGNLYFASDGHPGIGGLDLFVARRVNGQTTIENLGVPMNSSADDFGLYLFK
                     IDRGFFSSSRDGGKGDDDIYTFLNDDPNLKIVNYFLTGVTKSRDQEGNEKILPNVAVK
                     LLRDERLDTQEMDEMVTGNDGKFNFRIYPHENYILVGEKPPTDVRYFTTRLSYTTIGK
                     GVPQEELKELVTNITLDTTLVLDQIVIDKAIVLENIYYNFGSADIRPDAAQELDKLVN
                     ILLDNPEIAIELSSHTDSVDTEQYNLRLSSNRAKAAVDYIIRQGISKDRIIAKGYGES
                     RPIAANTNPDGSDNEEGRQKNRRTEFKVIRIDQIQSRQEELFDEDRFFQEEGSGAKKQ
                     GQGN"
     gene            complement(12358..13389)
                     /locus_tag="VGA21_11235"
     CDS             complement(12358..13389)
                     /locus_tag="VGA21_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015264559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HEX9651126.1"
                     /translation="MKKLFGALLFTIMITNNSCQEPYSNEDQISTIGPLTLIIHGGAG
                     TILRQNMTTELENEYREKLKEALNAGYAILDAGGSSRDAVIASIIIMEDSPLFNAGKG
                     AVFTSEGKNEMDAAVMDGNTGMAGAVAGIKVIKNPILAAIEVMENSPHVMMSGKGAET
                     FAIEQGLETADSAYFYTQHRYDQLLRAKSRDAATGLHNSFTDTKFGTVGAVALDKSGN
                     IFAGTSTGGTTNKKWGRIGDAPIIGAGTYAENATCGISCTGAGEYFIRSVVAHDVAAI
                     MRYTGKSVRDAAEEVVMKKLVGKNGDGGLIALDRKGNFALTFNTPGMYRGYINEKGRA
                     KVMIYKDEE"
     gene            complement(13401..13838)
                     /locus_tag="VGA21_11240"
     CDS             complement(13401..13838)
                     /locus_tag="VGA21_11240"
                     /inference="COORDINATES: protein motif:HMM:NF018076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEX9651127.1"
                     /translation="MESRRGGERKLEQEMRFARCGTGILSIIASKLSASKITCFDIED
                     WAMENTRENFEVNDTRARIFHGSIDALHDQSYDVILANINKKVLLDQMKYYAAHLKSG
                     GILMVSGFYLEDLKNLAETAGRYRLKKIGESVKNNRALLILGN"
     gene            complement(14029..14796)
                     /gene="tpiA"
                     /locus_tag="VGA21_11245"
     CDS             complement(14029..14796)
                     /gene="tpiA"
                     /locus_tag="VGA21_11245"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014771715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEX9651128.1"
                     /translation="MRRKIVAGNWKMNNDLHEGLKLASEVFNMVNEEVRNDVLVLLAP
                     PAIHLYGLKNLMNNNSRVQLAAQNCSEHVSGAFTGEISATMIRSCGAKYVILGHSERR
                     QYFAESNEMLARKVDQALTNDLRPIFCCGESSGVRDKGEHIAYVSNQIEESLFHLDEK
                     SFGRTVIAYEPIWAIGTGVNATSGQAQEMHKSIRQFVQQKYGKTIAGDLSILYGGSCK
                     ASNASELFACQDVDGGLIGGASLQSIEFVNIAKSFPE"
     gene            14983..16884
                     /locus_tag="VGA21_11250"
     CDS             14983..16884
                     /locus_tag="VGA21_11250"
                     /inference="COORDINATES: protein motif:HMM:NF015213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HEX9651129.1"
                     /translation="MALLGTILILISGILANVYSKANVTPDTAQNARMLINLLDYIGK
                     DYSNAVQDGQIINEYEYEEMLEFSRQSKFFYGEISESVPRQDSLNNQFDSLLHMIVEK
                     SDSKFIAELAAQIKREILALNLVEISPDIWPSVIAGQKLYMAQCSSCHGEKGDGKGLA
                     SAGLEPAPTNFTDVEIMNAFSPFQAYNVIQTGIPGTSMISYSELNDKEIWDLAFYINS
                     IQFEEKNNHQNPEIPLAEVSSLTNNELTKKYNLDEEDVRALRLFVPSQSMSALAIARQ
                     YLNATVESYRKGEKEEARNNSLKAYLEGIEPVEHRIKASDNSLVNKLEKSMMDIRSAI
                     YQNKDVKEIESLTLIAYANIEKADEIISGQKNSIGFTAFMSASILLREGLEAFLIIIA
                     IISILRSMQANKAVRWVHSGWITAVVVGIGSWFFTDQLISWGAQSRELMEGAIALFAV
                     FVLIYIGFWMHRKTEMSRWKIFVEEKVKGLVKRNNLIGLAVFSFMVVFREAFESVIFL
                     SSWSLESGKENEAGIWSGVAIAATLLVLICWTLLRFINRIPLRQFFVYFSLVVVFMAT
                     ILAGEGVHALQESGYSTVTSLPFNLRLGVFGIYPTIETLLAQFAVIGLVIFLWKLNLG
                     SSKVKKPAK"
     gene            complement(16865..17908)
                     /locus_tag="VGA21_11255"
     CDS             complement(16865..17908)
                     /locus_tag="VGA21_11255"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase family protein"
                     /protein_id="HEX9651130.1"
                     /translation="MKGIAFHGIEDVRVEQFPDPEIVDPDDVIVNVKRTSICGSDLHV
                     YYGREKGLDEGTILGHEFVGEVMEAGPASKWKVGQRVMSPFTTSCGDCYYCKLGLTSR
                     CEQGSLFGWVEQGRGLHGAQAEYVKVPLSNSTLVEIPYDLSWEDGLFLGDIFSTGFYC
                     AEQAEIKPYGVYAVLGCGPVGLMTIAASRLLGAEKIFAVDAVEQRLQKAKELGAIPIS
                     LDENVTEIIREATEGRGADAVMEGVGSFKAGRNAYDLLRAGGIISTIGVCTSNDFYFN
                     PAEAYEKNVTFKVGRCPARYYMDKLIPVLQEGKVSPESIITQRMSLTEGRKAYDIFAN
                     KKEGCLKIILLAS"
     gene            complement(17908..19263)
                     /gene="gdhA"
                     /locus_tag="VGA21_11260"
     CDS             complement(17908..19263)
                     /gene="gdhA"
                     /locus_tag="VGA21_11260"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013454014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HEX9651131.1"
                     /translation="MQESSILNDLLKRSPGEKEFHQAVEEVFLTIGPVLEKEKKYRDA
                     KIMERMIEPERVITFRVTWTNDKNEIQINRGFRIQMNSAIGPYKGGLRFHPSVNQSIL
                     KFLAFEQVFKNALTGLPMGGGKGGSDFDPKGKSEAEVMRFCQAFMGELSRHIGHRTDV
                     PAGDIGVGGREIGYLFGAYKKIENQFTGVLTGKGAGWGGSLIRPEATGYGLVYFAQNM
                     LKNIGKDIKGKTCLVSGSGNVAQFAIQKIIQLGGKPVSSSDSSGFIYDEAGINQEKLD
                     FIKELKNVKRGRISEYADKYKSATFTAVPGDAGSNPLWDIKADCAFPCATQNEINATD
                     ASNLVRNGLALLGEGANMPTTKEATDIFLDNGVLFSPGKASNAGGVATSGLEMVQNYS
                     GIYWRAEEVDARLQEIMKNIHDTCLNVSEKYGQKGNYIAGANIAGFTKVADSMIAQGI
                     V"
     gene            complement(19351..22161)
                     /gene="uvrA"
                     /locus_tag="VGA21_11265"
     CDS             complement(19351..22161)
                     /gene="uvrA"
                     /locus_tag="VGA21_11265"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017731056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEX9651132.1"
                     /translation="MKKTGVLTEKSPLDSLDPKKFIIIKGARVNNLKNLSVAIPRNQL
                     VIITGLSGSGKSSLAFDTLFAEGQRMYVESLSSYARQFLGRMEKPEVDYIRGVSPAIA
                     IEQKVNTRNPRSTVGTSTEIYDYLKLLFARVGATISPVSGKEVKRDTVSDVVDFISSQ
                     ETNTRYTISCPLKIPDNRTLDNELKLLLSKGYSRVVIEGELVFIEDLIKVKKNSRTRE
                     INILIDRGVVKNDEDSQFRISDSVQTAFFEGHGECRVEMEGRMSKSFSDKFERDGMQF
                     EIPSVNFFSFNNPFGACRTCEGFGKILGIDEDLVIPDKSLSLYEGAIAPWRSQKMKKW
                     LTPLVKKAAEFDFPIHRPFSELSRKEIDLLWRGNKHFKGIDAFFNYVRTKSHKIQYRV
                     LLSRYRGRTTCPDCRGTRLRKDASYVKIQNQSITDLVLLPVNELTSFFEKMKLNKFQQ
                     KVAGRVIREIKSRLEYLQKVGLEYLTLNRLTNTLSGGEFQRIKLATSLGSALVGSMYI
                     LDEPSIGLHPRDTGRLIEVLKNLRDLGNSVIVVEHEEEIMLAADQIIDIGPEAGRLGG
                     QLVFQGKLEELNGHPLTHTLRYLNGIDKIKVPKNRRKWSKSIVLKGARENNLKNIDVE
                     IPLGVLTTITGVSGSGKSTLVKNILYPSIGKILGTVAEQTGKYDKLEGDYKKITQIEF
                     VDQHPIGKSSRSNPVTYVKAYDGIRQLMADQSLSRQRGYQPKHFSFNVDGGRCDACQG
                     EGTQKIEMQFMADIFLTCESCHGKRFKSEILEVTYNDKNIAEILDLTVDEALVFFEEL
                     NSVYSKLLPLQKVGLGYVRLGQSSNSLSGGEAQRVKLASFLGKTDPGNHVLFIFDEPT
                     TGLHFHDIHKLLTAIQALVDQGNSVVIIEHNVEVIKCSDWVIDLGPEGGELGGNIIYS
                     GTPEGLIKVTKSYTARYLKSKL"
     gene            complement(22499..22720)
                     /locus_tag="VGA21_11270"
     CDS             complement(22499..22720)
                     /locus_tag="VGA21_11270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651133.1"
                     /translation="MNKHLNLNSKHEEELVIIKSDNSINYRFKDTVVYESFDGVNEKL
                     DTVYSYYRNDWKMLYNGKFELIRETDSKF"
     gene            complement(22965..24533)
                     /locus_tag="VGA21_11275"
     CDS             complement(22965..24533)
                     /locus_tag="VGA21_11275"
                     /inference="COORDINATES: protein motif:HMM:NF037015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEX9651134.1"
                     /translation="MKKITLNEITLLITCIFFIFSSCQDKSKDTTDPIISFTNLDDNQ
                     QIRGDFTLEFNATDDREVHKIDFLIDDVMIETISGNDPYQFEINTGDYEDGQHAIKAI
                     VSDSEGNSAFANVSFNIRNLMLTVQAENYFEENSTGYIIIYDMDGEAVEYHELNNNST
                     KDFIYPNNYDGWDFNATIFVASHYGNNKFFYAITVQRCKSSNWILPSYNETEGYNQNL
                     TFSNIPAHDLAIVSNGFKSQVFSPLFNYPLFTKWNPANIFIGLISGQTASYKWIENAE
                     AGNINIDLTQTEEMSKNEVATQKNIFDNYIYGNLSVNDETRSYLLSYTEQQEYFYIPG
                     FESYTTIIGFGSDSKEVYVIKKGDLLTTLEDPEFDVNITSADNSGFTLTHTGDFDYYM
                     ADWSYYDHSNEPYVVTFYTIGGPEDEPKVFPIPAPIMNTYPSLNLSMLEPYYLSAVDL
                     KDVEDYDQISGSNNIDFYFEEPTKEGIYYFLSPNPDGGRFGLSDKEQNKNRDNSLIQR
                     LKNKKLLPGRNPGE"
     gene            complement(24834..26036)
                     /locus_tag="VGA21_11280"
     CDS             complement(24834..26036)
                     /locus_tag="VGA21_11280"
                     /inference="COORDINATES: protein motif:HMM:NF024704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YARHG domain-containing protein"
                     /protein_id="HEX9651135.1"
                     /translation="MKYSSFFLILILLLSKALFAQNNIIKDSDTLKYLIVQDIDTLHQ
                     VRINYYAPRKKNERDRLIYLNATLFDSSGINFDKDIHYFKDTFRGFQFDYIDKIYRVF
                     SLNIGKRGLNKLLESRSFTPRTFHFGSNKSDQLLELAEDGNVSVFADLNPYLDYKEEY
                     RSLEEIWKIHELSKEKILCESCAWGGGCTLYRYFIIRKDSVEEINFDQLRQPYKNQFE
                     PQYEDFRVNFYFSHYDYDFLMAAVDYGNLPENTDLIFSKDLNDTTHILKKHEHWKVIG
                     ENIQEGHNEFFYLRSILDNKEKVIVNYLINKDFEKITYQLFKGHTVTKRELNNFNSDQ
                     LNILKNLIFAKYNYAFDNEFYQAYFNLFAFYNEPEKLISRTKEVNNNLTKTDVENLAI
                     INALVRKK"
     gene            complement(26033..27334)
                     /locus_tag="VGA21_11285"
     CDS             complement(26033..27334)
                     /locus_tag="VGA21_11285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651136.1"
                     /translation="MKNKILLICLFFCQAAYLGAMNKPSVYDPLGKLSTEERNRIEAE
                     GRTLSANRKVNGQDLIIQIDFMSEDEFEEGGVTGRDESGLTLFSLGTYINWSLRYFSQ
                     PAVLMLFLDQGNGNYVLDALNFSDMLMDEGIPDLVRRYIRDEIMKKENSYTETISSGL
                     YAIGEALNPTYRERLEQKGNEMLNTDDRYHRAHCVYGDCHYYEFTPEEKSVLGPTQPK
                     DAITFHGLPESRPDNLTSVVNEIVDWGYTHENEIASNWPWIYDNNENKYQLNAVTPTG
                     YFTVKKDNPWSVLNTISNTKDIGELLPTMGWLQIHGWRATYCNFFAQDLSYYVLGHLP
                     WATGTVANKIHDYIVSSQNFIGIPKSEINTYVDNGFMVYITINQNPAAGHIAMGWMSN
                     AENKEIIQAGRTTGILMVEEGFGDEVDASINLYLGFLKKVI"
     gene            complement(27324..>28196)
                     /locus_tag="VGA21_11290"
     CDS             complement(27324..>28196)
                     /locus_tag="VGA21_11290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651137.1"
                     /translation="NEDYQMTHGEIVTVHNPDSPFVYDGRKDRTETTETTTTTSTSSA
                     TTTAIASTEITIENEIDSVFINDDGKIVVLDTAGNATTHEIPKDENGEDKAVTITDSA
                     GNSYTVEGGEVKKGVSGEGNTPVGNTEEERNLTGLDSLVYAAIIHQIEASEATIDSIK
                     GQMQPFIEAIEGIIESENFYPPRIRGENDELIGEGISLHIAIVDKPENIRSRLSQSVK
                     SIDEARNHLIQLDIPIQELKPTIELLNELKEGEPFEELVESIRNDSGLPESSPEKIKY
                     IEGVINQRINENEE"
BASE COUNT         8150 a         5905 c         5445 g         8696 t
ORIGIN      
        1 cgaagataat tttgaaattt actaattcgt tttcggggaa tgtttgcaat ttgagcagga
       61 caaccggaag tgatgcagcc attaatcctt taaccacatc aattagcaga acaattgttc
      121 cggctctttt gcccaggaca cgaaatgtat tagtggcacc cggattgcca ctcccgtgtt
      181 cccgaacatc aatgccatga tataatttgc cgaaccaaac cgcggtgggt accgcaccca
      241 ataaataagc gatcaaaatt ccggcagtaa gcaaaaagat ttccattgtt taggttcttg
      301 gggataccaa atatacgata ttggtttaat agagaaaatt accggttgcc gtcttttttc
      361 ttttttcctc caaaaccatc atcagtcaga tcttcttcaa tcggggccgc atttaattgg
      421 tagggttgct ggatattcag gtaatcattt cgaaaacggt ttacaaaggt gagtatttcc
      481 gattcatctc cagggataaa cacaaattct ccaatcttag ctttgcccgc atttgatttg
      541 gtcgcgataa actcattgta tgcaggattg gaagagtatg ttatcagctt attattctca
      601 taactgaaat aataccagga gtcaactgat gctttcagaa ataaatcaat gcgctcaccg
      661 tccaggcgtt taataatttc cagaaatcct tcaaacgaac catttatatc ttctcttaga
      721 atatttgaca ctccaattcc accgacacta tagaatgctt tattaatatc agaccatttc
      781 agatctacat tgcttaaaac gatgggttta actacggccg gcgagatgct taccaggggt
      841 atgtacacca tggccgattg ttcttcataa gtcttagcca ccttgtctcc aaaaaggttg
      901 gcaattttat aaagcagagt tgttttatcg ccaagggctt ccggagcgcc atacagatta
      961 atcgaattct tcaaatcgac tgccatcaga tcaaacgccg attgaggtat gttaaaaatt
     1021 attgacatga atgcattaaa atcgaactcg ttagtttcga gatttcctgt accgacaccc
     1081 gcggcagtaa tgcttcttga tttatcattt tccagaaact gaagagttcc gtcaaacgat
     1141 atgtcgccgg tttcttcatt aaaagtaaat gactttccgg caagggtctc acctgattct
     1201 ttttgcggag atatgataat gaattgcgcg ctatcctgca agtatttcag attgccgccc
     1261 ggacggaaga aatcttcatc atcataattc cgcttgtcgg taacgaaggt gctatacaag
     1321 ctgaaatcat tcacatcgaa atgtaatccc gcggaaacat taagtccatt ttctgtaaca
     1381 ctattatcaa aatcaaaaat cacttcctgt tgttcagcgg cagactgata ccgaatccat
     1441 gtatcatatc ccgatatttt ctttagatca agtttcacgt aaccatcaag ttcaagtgcc
     1501 gggctgttgg ctatcatttt aacatcacct ttatagtaca tgcccgggga aattaagagg
     1561 ttgtcatcct gtgaaattcc tccattggca actgtgtgga attcctgtct tcttttattg
     1621 gcagacaagt cttccagtct gaagttggtc aatttaattg caaaagtatc ttccgaagca
     1681 tttacaaatt gataggtggc ttcaccctca aatttcttcc tggagataat agtaattgta
     1741 ccatcataca ggcgatggta ttcattcaaa gtatcaataa ctaaagtggt attcttaaga
     1801 gtaccaatct gtgcattctc aagtataagt acttctccgc cttcaggagt tattttagca
     1861 tcagcgactg ttatgaaagg gattccgctt acttttaatt ccagcttctt aatatcataa
     1921 actgcttccg tcgcgtaaaa tgcaagtgag tccaaatctt tccgggtagt gtagaagtaa
     1981 gaatcctcta gctggaattg agcaggtttt gccattttta caatggaact atccaaatcc
     2041 caaactgcgc ttgggatgga ggttttaaat tgagcaaaag gaaattccaa aacatcatca
     2101 ccggcaacct ccggggttat ttctgcaaag ttttgtgtga ggtcaaaatt cagtttcaca
     2161 ccatccgctt tgaggacggg tttttccgga ttggaggact taacttcaaa atctgcctgc
     2221 cgggctgaaa aattattctg gttaaaggca taccgggatg aaactgactg tgatcccctt
     2281 gtaaggagag tgccggaacc cttaataccg cctttggtta tgttggcagt tccatccagc
     2341 gtcgcggtgt tatcataaat ttgaattggt gccttcaaat tagtaatata catactgtct
     2401 tttctgggca gccatcggag tttatagttc tccacgtaaa gtttgggaaa tgaagtgcct
     2461 aagtgagttt ctttccgcac ttccgcaacc gagccttcag tagttacaga atccacataa
     2521 aagataaagt catttgaaat aagatcggtt gtgagatatt ttatttctcc attgccacgg
     2581 attccagctt tatctaattt catcttatta aaaaatcttg ccgtagtatt atatagcggg
     2641 taacctgaag gaggcaatga atgttcaaac cctaatgaga agtcaggctg tattttaaga
     2701 gattctttga aatcgggaaa catgccgctg ctgccaaaag ttccggtaaa accaattgat
     2761 gatggatcgc tattgctcag gctatcaagc ttaaaaggcg gcacaaggaa atatacagac
     2821 cggttataaa cgccgccaag atattcgggc ctgttaaaat agactatcga gctggagccg
     2881 gtgccatcaa atttcggata atcgggaaat atttttctac cggatttatt atttgcacga
     2941 ttgataaata atgttccgga tgtcgccgcc atagtgctgc catcctccga ggcaatagag
     3001 tcttcaggag ttatacccga gatggcgttg tctacttttc tcctcctgct gcctcttgag
     3061 tcctcttcca gaacatagaa ttcaatggaa tcaatttgat tcaactccag agtgaatgcg
     3121 tcgtaattaa atgtgaaatc acgaccgatg tactcaaaat tgccggcaaa tactttcccg
     3181 tcaaacttaa aagtgcggtt ttcaagcaat gtaattgtac tactatcggg ctgaatataa
     3241 acatccagta tttcactaat ataaaatttc tcaatccctc gcacaacaat ttgttcattg
     3301 gccatatcaa aggttgcatt cgaattggta ttagtgaggg atagcatcct gatattgtca
     3361 aaatcttttt tgcttttctg ggcatccgtc gtgtgcaggg ctttttcttt tattgttact
     3421 tcctgcgtat ttatattgta atctataaat cctgcccaca ttaaatagaa aagagaagac
     3481 tttatggtat ttctctccag accggtgaat ttctccagag attctaaata gatgggggag
     3541 ccttttttat tataataacc cattaccatg cttagtggat tgaacttgta caaggcaatt
     3601 gatcccgtaa cctgatcggg gttataatac tccttcgatt cgaagacagc cgggatttca
     3661 tccctggcat tcaaaatgga aatttcaaga ctgtcagagt tcacttccca cctcacaatg
     3721 tcagcaataa aatccgtgtt aaaaaaactt gccgagtatg gcgttctttt gaaaccaccc
     3781 ttatcttttt gaagtatcag atcatcggtt ttagtattat aagaaacacg cacagttggg
     3841 tgggtgattg aatcgcgttc atgataaatt acgatggctg ccctttccgc cgtaatcaga
     3901 ctgtcaccga gttcaaaaag tctggaccgg gctttaaatt tcttcacgcc atttttggtg
     3961 acttcaattt tagacaaccc ttcaaaaatt gaactactta ctattctttt gccattaagt
     4021 gaaaaaccgc cggtatagga taaatcttca ttgccaattc caaccactgg aatattattt
     4081 ctgtatgaaa taaaccgggg ataggaagtt ttgtttgtga caagaccatt gaatgagaat
     4141 atcccctcaa ttggttcagc aaccttgccg gaataagtaa gtgtaacatt ctcggcttca
     4201 ataaccggcc gttttgtgtc aaagttatat tttgaaagtg ttacaaatac tgtatcacca
     4261 agaccggttc ttgaccaatc aaatttaccg ccttctccaa taaacattcc gtcctttatc
     4321 gtaaaactac ccgctgtttt ggagagagtc aatgctgaat ctatctgcgt agtaaatacc
     4381 aaatccaatt tcgaaaaaac aattgtaggt ccacttaccg gaggctgaac ggcatcctgg
     4441 attaaaacgt cttcaagagt ttgaaccggc tcctcctcac cggatttttc atcaccccat
     4501 tcattacccc actccgatcc ccattcttct tctgcaggct cagagtccca gctttcgaag
     4561 gctcctgtgg ctgtgtttac ttctttcttc tgaatttccg cctcttcttc aatttccaaa
     4621 tccaggattg tgacgctgcc aacatattca aaagtatatt catcattagt tacaatcaga
     4681 ttagaaaagt tggagtaata aagagcacgg aattcaaaaa aattacgcat actgttaaag
     4741 taagtcatca agtcccggct ttcgaaggac tttatcactt cctccgtcat attcatgtag
     4801 ctcagcaatt tggtttgatt taatccttcc tgatcgatgg ccattaccag tgtttcaaga
     4861 tattccgata gtcgtccgtt cagcttgtaa cccttttcgc tcattatttt gaaatgggcg
     4921 atgagctttg tctggaggtc caggcctaaa tccgccttcc agattctgcc aacatctgaa
     4981 gcgagtgcta ccaggacatc ctggttggaa ttctccaatc ggttttgaag ttcggccaga
     5041 aatgtagccg gttcatccgg aaatgaggat gacctttgtg ccagagttgt gttaacggaa
     5101 aaaataccac caacccaaaa cagcaacccg atgataacct ttgaacttat tatcttctta
     5161 attggcaaat tttccggctt tgatttatta aaataaacga aaataacgca gatcttgggg
     5221 ttcagcaagg caaaaagttt tccattatgc acaatttagg acattcattt tatacaagtc
     5281 ctaccttttt gttttggtat agttacttta tttttgcggc tactatggca aagggaagaa
     5341 aaaagaagga aaagacgcga ttcacagaaa aggagttgaa ggagtttgag accattatca
     5401 atgataagct tgacaaggcc agaggtgaat tcaaagtttt aaaggaaacc ctcaacagaa
     5461 ccaatgattc agggacagac aataccgccg gcacactaaa gatacttgaa gacggggcag
     5521 atacacttga gaaggaaagt ctgagccaac tggcagccag gcagcaaaaa ttcatcgccc
     5581 aactcgaaaa tgccctggtt agaataatga atggcactta tggcgtttgc gtggataccg
     5641 gcaaattgat aagtaaagaa aggttacgtg cagtgccaca caccatgcat tcgattgagg
     5701 ctaaacttaa tcgcgaccga tagttgaact ttcttaataa ccatattgag gcgttagtct
     5761 tttgttctcc ttcacccata aaagttaagg aattaatgga atgtctttca gaaatgtttg
     5821 gagccgatat tccggaggaa gatataacaa aagctcttga agatgtgaag aataaatatg
     5881 aggatgaagc attctccctg caattggtac acaccggcgg agggtatcag ttcctaacca
     5941 agcctgccta ccaatccagt atttcgattt tattgaaaca ccagtccagg aagaggctct
     6001 ctacctcggc acttgaaacc ttatcaatta tcgcttacaa gcaacctgta tccaaacctc
     6061 agattgaaca aattcgcgga gtcaattgcg actacagtat tcaaaaactt cttgacaaag
     6121 gactgattga aattaaaggt aaggcggact cggtaggtaa gcccatcatc tatggaactt
     6181 cagaattctt tatggattat tttgggatca atgatctcca ggaattgccc acaccaaaag
     6241 attttcaggc agagcaaaat actatcggag aagaaaagta aattctccgc attaatcatt
     6301 tatcttttta gcattgaaga ggaaaaaatc cggcaacccg tcaaaatcac ccccatctga
     6361 attaatccgg ctgaacaggt ttattgctaa cagtggagtt tgctcacgca gagaagccga
     6421 tcaattgata gaacggggat taattaaagt aaataaggaa gtaattactc agcttggcta
     6481 taaagtgaga cgcggagatg cggtttttta taaaggtaaa aaattatctc ctgaaaaata
     6541 cgtatatgta ctcctgaaca agcccaagga ttacatcact acagccaaag atcctgaagg
     6601 aaggaaaact gtaatggagc ttgtttccaa tgcctgcagt gagcggatac tacctgtggg
     6661 aagattggat agaaatacta ccggactttt gttgttcacc aatcatggcg aacttgccag
     6721 gaagttgtca catccttcaa ataatgtccg gaaaatttac cgggttactg ttgataaacc
     6781 tatttcaaaa tcagatttag atgctattgc cgtaggagtg aaacttgaag atggacttgt
     6841 tgagatagat gacctggcta ttttgaatac gcaaaaaact gaacttggcc ttgagctgca
     6901 tagtggaagg aatagaattg tccgaagatt atttgaatca ttgggttatg atgttgtaaa
     6961 actggatcgt gctgtatatg ccggccttac caaaaaaaat ctttcccgcg gaagatggcg
     7021 atttttgaca gaaagagaag ttgggatgct gaacaaagga ttatgattga tctcaggcat
     7081 ttcaaattat gccattttga aaattaaaat tccccgtttc tagttttgaa aattcagagg
     7141 gtggttcccg ctggtcaaca gtcaccccat gcagaagctt tgtggtgatg agtgggatcg
     7201 aaccaccgac acacggattt tcagtccgtt gctctaccaa ctgagctaca tcaccggtaa
     7261 aaaaattccg acaaaagtat gataatttat taactgtctc caagtatttt aagaaaatat
     7321 cggtaaaatt tgaacagaat cgaatacttt tataaacatg cttctcgtgc aacagattac
     7381 tttcatcatc gtacttgccg tggcaatttt tttcctgcga caaagggttt tgatgattcg
     7441 aagaaacatc ctgatgggta aaaaccttga tcgttccgac cgatcatcag caagatggca
     7501 caccatgctt ctggttgcct ttggacagaa aaaaatgttc aagaatttta ttccagcctt
     7561 ccttcattta ttgatttacc tcgggttcat cctcattaac attgaagtac tggaaattgt
     7621 tcttgatgga atcctgggaa cacatcgctt gtttgcccct ttcctgggag acttctataa
     7681 ttttctgatc aatttctttg aattccttgc cctgggagtg ctggttgcct gcataatctt
     7741 cctgattaga aggaatgtgc ttaaactgaa acgattccac tctgcggaaa tgactgcctg
     7801 gcccaggctg gatgcaaacc ttattctgat tattgaatgt tttttgatgg tcgcgatcct
     7861 ttccatgaat gcagctgatc agcttttaca acaagttcat gccgatggct atactcaaac
     7921 cggatcgttt attttttcag ccgcattatt cccactcttt gaaagcttgt cacagacagg
     7981 tttgctggtt cttgaacgtt ttgcctggtg gtttcatata attggtattc ttgcctttgc
     8041 catttacatt acgtacagca aacatttaca catctttatg gcattcccaa atacctacta
     8101 ctctaatctg gaatccaggg gaaaaatgga tattatgccc actgttacca atgaggtgaa
     8161 actgatgttg ggcttacagt cggaaagtag cattccacca cctacggaag cagaaaggtt
     8221 cggtgcaaag gatgtcacgg atctttattg gaagaatttg atggatgcat acgcgtgtac
     8281 agagtgcggc cgatgtactg cagagtgtcc cgcaaacctt accggtaaga aactttcacc
     8341 cagaaaaatt atgatggaca cacgggacag aacagaagaa ttgggtaatc ataaaagtaa
     8401 aaatactact gactcaaaat ctttgcttgg cgattatatt acaaaggaag aaattaacgc
     8461 atgcaccagt tgcaatgcgt gtgtggaagc atgccccgtc aatattgatc ccttgtccat
     8521 tattctgcag ttgcgcaggt atgtgacaat ggaagaatcc gcggcgcctg cctcctggaa
     8581 ttcaatgttc caaaatattg aaaccaactt tgcaccctgg aaattctccc cccaggacag
     8641 atttaactgg gctgaacctt tgaaggaaaa aaaacaatga gcgattctgc aatatcatac
     8701 tccgtcccta ctgtagctga aatggccgcc aaaagagagg ttccggaaat tttattctgg
     8761 gtaggctgtt caggatcgtt tgatgaccgt tccaaaaaaa ttacgattgc gcttataaaa
     8821 atactgaatc atgcagggat taaattcgcg gtgttaggcg ctgaagaaag ttgcacaggg
     8881 gatccagcac ggagagcagg caatgaattt ctatttcaaa tgcaggcaat gaacaatatc
     8941 cggattctaa atggatacaa tattaagaaa attgtaaccg catgcccgca ctgtttcaat
     9001 accctgaaaa atgaatatcc tgaattggga ggaaactacg aagttgttca tcattctcaa
     9061 ttcattcaac aattaattga tgaaggtaaa ctaaagatga aggaggacgg agaattcaag
     9121 ggtagcaaaa ttacctacca tgattcctgc tttttaggac gcgcgaataa tgtatacgaa
     9181 gctccacgaa aagtattgga agcattggat gctgatttaa ttgaaatgaa acgttgcaaa
     9241 acaaagggat tgtgttgcgg tgccggtggt gcgcaaatgt ttaaagatgc agaacccgga
     9301 aagaaagaag taaacaccga gcgcacggaa gaagctttgg aaactggtgc caaagtaatt
     9361 gcgtcggcat gcccgttttg tatgaccatg ctctccgatg gtgtcaaaac ccgggaaaaa
     9421 gaaaatgaaa taaaaattct ggatttagcg gaacttattt ccggcaatct gaatttataa
     9481 aagaaaggag actactatgt ttgttgattt tgaaaatttg cctggtgacg cgaagatttg
     9541 gatttatgcc ggtaataaac gtttcaccgg aaatcaaaga gaatggatgt ccgaagctct
     9601 gcaggaattt cttgaaaaat ggacctcaca tggcaatgat ttaaaaagcg ggttcaaggt
     9661 gctttatgat caaattctgg taattggtct tgatgaaaat gctgcggatg caagtggttg
     9721 ttccatcgat gcttcagttc atttcattaa ggaaattgga gagcacctgg aagtcaattt
     9781 ttttaatcga aacctggttc cgtttctcca tataaataac ttaaccctgg tgaacctcaa
     9841 tagcctgaaa actaaaattg ccaacggaga aattaccgga gaaagtacaa ttttcaacac
     9901 cctgttagca aaaaagagcg atttgaatca aagcgtaatc ccattgaaag acagctggct
     9961 aaatcgttac ctgaaaaacg aggaaatctc aacctaggtt ggcggaattt ttgctctggt
    10021 ggcagcgaac attcatcgcc agatatgtgt agatttgtat caatgaagaa tttggtgaaa
    10081 atcattctta ttgccgcttt tatcacgggc gcaagcttat cttttggaca gagcaaggcg
    10141 ttaaagaaag gggtgaagaa aatcactaag ggagaatacg acctggctat ttctcaattc
    10201 caaaaatata cagacagccg cgaacttggt ggtgaggcta atttttattt aggcgaatgc
    10261 taccggcttt caaaccgggt tcatcaggct gaagattatt acaagaaggc tatcgagcgt
    10321 ggctaccgcg cggaagaggt ttatttttat tatgccttcg cgctcaaaat gaatcgcaag
    10381 tataaagatg cccagattca gctggaaaga tttttgaatg ttgctgagaa taaagaactc
    10441 atcaagcatg caaatgtgga actggataac ctggcaaaga ttgatgaaat tttgaataag
    10501 aaaacttact atacggtcgc ttcccttgaa gatctgaata ctgagaacgc agagtatagt
    10561 cccgtatatc aggatggatt tctatacttc accagcaatc gaggtgttga taagatatac
    10621 aaagctaccg gcaccggttt tacagatctt tttcgtgtat caacacaggg agccaatgta
    10681 gagattcgtt cgcttgaaaa tctgggcgaa gaaattaatc tcttcaatgt aaatgaaggt
    10741 tctgtaacat ttactcctga tggccgtacg atggttttcg caagaggaaa ttccgggaag
    10801 cgtaaaggtt ctgaaaatgt ggatctattt attacacgat tcagaaatcg tgaatggtcc
    10861 gatgcaaggc cgataaatat taatgacaac ggtgcctggg attctacacc ggcgtttagt
    10921 gcaaatggca gaattctcta ctttgcttcc gacagaaaag gaggctatgg cggagttgac
    10981 ctgtacgctg caactattga tcgtaccggt agatttaccc gggttagaaa tcttggcccg
    11041 gaggtaaata cgcctggcaa tgaaatgttc ccctacgtgg ctgatgatgg taatttatac
    11101 ttcgcttcag atggtcatcc cggaatcggg ggccttgacc tattcgtagc caggcgagtg
    11161 aatgggcaaa ccaccatcga gaatctgggt gttcctatga attccagcgc tgatgacttt
    11221 ggtctttacc tcttcaaaat tgatcgcgga ttcttcagca gcagcagaga cggtggaaag
    11281 ggtgatgacg atatttacac attcctgaat gatgatccta atcttaaaat tgtgaattac
    11341 ttccttaccg gagttactaa aagtcgtgac caggaaggaa atgaaaaaat actccccaat
    11401 gtagctgtaa aactactccg ggatgaaaga cttgacactc aggaaatgga tgaaatggtt
    11461 acaggtaatg atggtaagtt caattttaga atttatccgc atgagaatta tatcctggtt
    11521 ggcgaaaaac ctccaacaga tgtgcgctac ttcactacaa gactcagcta cacaacaata
    11581 ggcaagggag taccacagga agaattgaaa gaactggtaa ccaatattac tttggataca
    11641 acgctagtgc ttgaccaaat cgtaattgac aaggctattg ttctggaaaa catttactat
    11701 aattttggaa gcgctgatat ccggcctgat gccgcccagg aacttgataa gcttgtaaat
    11761 atccttcttg acaatcctga gattgccatt gagctcagtt cacataccga tagtgttgac
    11821 acagagcaat acaacctgcg actaagttct aatcgcgcca aggctgccgt agattatatt
    11881 ataaggcagg gtattagtaa ggataggatt attgccaagg ggtatgggga atccagacct
    11941 attgcggcta atacaaaccc tgatggcagc gataatgaag aaggtcgcca aaaaaaccgg
    12001 aggacggagt tcaaagtaat ccgcattgat caaattcaaa gccgccagga agagctcttc
    12061 gatgaagatc gcttttttca ggaagaagga agcggagcga aaaagcaggg gcagggcaac
    12121 tgaagtttcc taagtttgag tcgatagtag ccttctgtta ttttacaagc agtatcgctc
    12181 ttttgtaaaa cccttaattg aagccttgcc gattgcttaa tttataaaat taaggaaaca
    12241 aaatgactca agacatccat tgggaacagc ggtattccaa ttttcttcaa acgagtgata
    12301 caaataaatg aaggtgcagc cattgaagtc gtaaagctga ttatagaagt caaattctca
    12361 ttcttcatcc ttatatatca tcacttttgc ccggcctttc tcgttaatat aacctctata
    12421 catcccaggc gtattgaaag tgagcgcaaa gtttcctttt ctgtcaagag ctatcagacc
    12481 gccgtcacca tttttcccta ccagtttttt catcacaact tcttcagcag catccctgac
    12541 tgattttcct gtatatctca ttatggcagc aacatcatga gcaacaacac ttctgataaa
    12601 atattcacct gcgccggtgc aggagattcc gcaagtagca ttctccgcat aagtgcccgc
    12661 accaatgatc ggtgcatcgc caatgcgacc ccatttcttg ttagtagttc cccccgtgga
    12721 ggttcccgca aaaatattcc ccgatttatc cagcgctacc gcccctactg taccgaattt
    12781 ggtatccgtg aacgaattgt gtaaaccagt tgctgcgtcc cgcgatttgg cccgcaggag
    12841 ttgatcatat ctgtgctggg tgtagaaata cgctgaatcc gctgtttcca gtccttgttc
    12901 aatagcaaag gtctcagcgc cttttcccga catcatcaca tgtggagagt tttccatcac
    12961 ctcgattgca gctaatatgg ggttttttat aactttaatt ccggcaaccg caccggccat
    13021 tccggtattg ccatccataa ccgccgcatc catttcattt tttccttctg aggtaaatac
    13081 tgctcctttt cctgcattaa aaagtggcga atcttccata attataatag aagcaattac
    13141 ggcatcacga cttgagccgc cggcatccag tatagcatac ccggcattaa gcgcttcctt
    13201 cagtttttcc cggtattcat tttccaattc cgttgtcata ttctggcgca ggattgtgcc
    13261 cgctccacca tgaattatca gtgtaagcgg tcctattgtt gaaatctgat cctcattgct
    13321 ataaggttcc tggcaggaat tgttggttat cataattgta aaaagtaatg ctccaaataa
    13381 ttttttcatc acaaagaatt ttaattacct aaaattaaca aagcccggtt gtttttaacg
    13441 ctttcaccaa tctttttcaa ccggtatctt ccggctgttt cagctaaatt ttttagatct
    13501 tccaaataaa atccacttac catcaatatc ccgccggatt ttagatgagc agcataatat
    13561 ttcatttgat ccaataaaac ctttttgttg atattggcca gaataacatc ataagattga
    13621 tcatgcagag catcgatgga gccatgaaag atccgggctc tggtatcatt tacctcaaaa
    13681 ttttcacggg tattttccat ggcccaatcc tcaatatcaa aacaggtaat tttacttgca
    13741 ctaagtttgg atgcgataat agacaatata ccggtaccac atcgggcaaa cctcatttct
    13801 tgttccagtt ttctttctcc acctcttcta ctttccactc caaaatttca ttttcataac
    13861 ggttgaaaaa tgctttccag tattttgcat atcaaattcg catattttga acagaaccca
    13921 gaaatcctgt ttctgtttcc tggaaggact tatgcctgta aaatttcaga gatagccggt
    13981 gtgcattgta tgatgactac cgcataatca gccatctggt agaagtactt attcaggaaa
    14041 agatttggca atgtttacaa actcgatgga ttgcagtgaa gctccgccaa tcagaccgcc
    14101 gtcaacatcc tgacaggcaa aaagctcaga tgcattggaa gctttgcaac tgcctccgta
    14161 caagatagaa agatctcccg cgattgtctt gccatatttc tgctgcacaa actgacggat
    14221 tgacttatgc atttcctggg cctgacctga ggttgcatta acaccggtgc caatagccca
    14281 aatgggttcg tatgcgatca ctgtccttcc aaaagatttt tcatcaagat gaaacaaact
    14341 ttcctcaatc tggttagaca cataagcaat gtgctctcct ttatcccgca ctcctgatga
    14401 ttctccacag caaaatatag gtcgtaaatc attggtcaaa gcctggtcga cttttctcgc
    14461 cagcatttca ttggactcag caaaatattg tcgccgttca ctgtgcccca ggataacata
    14521 ttttgcaccg catgaacgga tcattgtggc actaatttct ccggtgaaag caccagatac
    14581 atgttcactg caattttgag ccgcaagttg caccctggaa ttattattca tcaaattctt
    14641 cagcccatac aaatggatgg caggtggcgc taataatacc agcacatcat ttcttacttc
    14701 ttcatttacc atattaaaaa cttcggaagc cagtttcaaa ccttcatgta ggtcattatt
    14761 catcttccag tttccggcaa ctattttcct tctcatccca gcaaagataa gaattaacca
    14821 aaaagtaccg attatggttt aattaagttc tttatctaaa gtgcattcaa taaatctcaa
    14881 attaagggct ttagatatag cagaattcat aattctggag ttactttgca ttatttagaa
    14941 ttgatctaaa taagtttgga aaagttggtt aatatcctca aaatggcact tcttgggacg
    15001 atccttatcc tgattagcgg aatattagct aatgtatata gcaaggcaaa cgtgacacca
    15061 gacacagccc aaaatgcccg aatgctgatt aatttgctgg attatattgg taaggactat
    15121 tcaaatgcgg tgcaagacgg tcaaatcatc aatgagtatg aatatgaaga gatgcttgag
    15181 ttctccaggc agagtaaatt tttttacggt gaaatttctg aatcagttcc caggcaagat
    15241 tctttgaaca atcaattcga ttccttactg cacatgattg ttgaaaagtc agattcaaaa
    15301 ttcatcgctg aactggctgc acaaattaaa cgcgaaattc ttgcactaaa cctggttgaa
    15361 atctctcccg atatctggcc aagcgtaatc gccggacaaa agctttatat ggctcagtgc
    15421 agttcatgtc atggtgagaa aggagatgga aagggattag cctcggctgg acttgaaccg
    15481 gctccaacca atttcactga tgttgaaata atgaatgcgt tctctccttt ccaggcctac
    15541 aatgttattc agaccggtat ccccggaaca agcatgattt cctactcgga gctcaacgat
    15601 aaggaaatct gggatttggc attttacatc aattcaattc agttcgagga aaagaataat
    15661 catcagaatc ctgaaatccc gctagctgaa gtttcttcct taactaataa tgaactaacg
    15721 aagaaatata atctggatga ggaagatgtt agagctcttc gtctatttgt gccatcacaa
    15781 tctatgtcag ccctcgcgat tgcacgacaa tatcttaatg caacggtcga gtcatatcgt
    15841 aaaggagaaa aagaagaagc aaggaacaat tcgctgaaag catacctgga aggtatagaa
    15901 cctgttgagc accgaattaa agcatctgac aattcccttg ttaataaatt agagaaaagc
    15961 atgatggata ttcgatctgc catctaccaa aacaaagatg taaaagagat tgaatcgctt
    16021 actttaatcg catatgcaaa tattgagaag gctgatgaaa ttatcagcgg tcagaaaaac
    16081 agtatcggct ttacagcttt tatgtcagca tcgattttgc tgcgcgaggg tttggaagca
    16141 ttccttatta taatcgccat aatcagcatt cttcgatcca tgcaggcaaa taaagcggtg
    16201 cgttgggtgc acagtggctg gattaccgcc gttgtagttg gaattggcag ttggtttttc
    16261 actgaccagt taatctcctg gggagctcag agtcgtgaat taatggaggg agctattgca
    16321 ttgtttgctg tctttgtact gatctatatc gggttctgga tgcatcgcaa aactgaaatg
    16381 agccggtgga aaatttttgt tgaagaaaaa gtgaagggtt tggtgaaaag aaataacctg
    16441 attggtctgg ctgtattttc tttcatggta gtttttcgtg aagctttcga atctgtcatt
    16501 ttcctttctt cctggagcct ggagtctggt aaagagaatg aagctggaat ctggtcagga
    16561 gttgccattg ctgcaacatt gcttgtgctt atttgctgga cattgttacg gtttattaac
    16621 cgaattccgc tgaggcaatt ctttgtttat ttctccctgg ttgttgtttt tatggccaca
    16681 attctcgctg gtgagggtgt tcatgccttg caggaaagcg gctattctac ggtaacgtca
    16741 ctccctttta atcttcgtct gggagtattt ggaatttacc ctacaattga aacgttactt
    16801 gctcagtttg ctgttattgg tttggtcatt ttcttatgga aactaaatct tggaagttcg
    16861 aaagttaaga agccagcaaa ataattttca aacatccctc tttcttattt gcgaagatat
    16921 catatgcctt cctgccttca gttagagaca tcctttgcgt aataatactc tcaggactca
    16981 ctttaccttc ctgcaaaacc ggtataagtt tatccatata atatcgcgcc gggcagcgcc
    17041 cgactttaaa tgttacattt ttctcatagg cttcggcagg gttgaagtaa aaatcattgc
    17101 ttgtacagac tccaattgtt gatataatac cccctgcgcg caagaggtca tacgcatttc
    17161 ttcctgcttt aaagctgcca actccttcca tcactgcatc tgctcccctg ccctccgtag
    17221 cctctctgat tatttcagtt acattttcat ctaatgaaat cggaattgct cccagttctt
    17281 ttgctttttg caggcgttgt tccacggcat cgacggcaaa aatcttttct gcaccaagta
    17341 gccggcttgc cgctatagtc atcaatccga ccgggccaca accaagtacc gcataaactc
    17401 catagggttt aatttctgcc tgctctgcac aataaaaacc ggtagaaaaa atatctccaa
    17461 gaaataatcc atcctcccat gaaaggtcat aaggtatttc tactaatgtt gaattcgaaa
    17521 gtggtacctt cacatattca gcctgggcac catgtaatcc gcgtccttgt tccacccagc
    17581 caaaaagact tccctgctca caccggcttg ttagtccgag tttacagtaa tagcaatcac
    17641 cgcaacttgt agtgaatgga ctcatcactc gctgcccgac tttccatttg ctggcagggc
    17701 cggcctccat cacttcgcca acaaattcat gaccaagtat cgttccctca tccaacccct
    17761 tctccctgcc ataatacaca tgcaggtccg acccgcagat ggaagtccgc ttaacattta
    17821 caattacatc atcgggatca acaatttccg gatcaggaaa ttgttctact cgcacatcct
    17881 caattccatg aaaggctatg cctttcatta aacaattccc tgggcaatca tggagtctgc
    17941 aactttggtg aatcctgcaa tattggctcc ggctatataa tttccttttt gtccatattt
    18001 ctctgagaca ttcaggcaag tatcatggat gttcttcatt atttcctgta atctggcgtc
    18061 tacttcttcc gccctccaat aaataccact gtaattttga accatttcaa gacctgatgt
    18121 agccactccg ccggcattcg aagccttacc gggactgaaa agtacaccgt tatccagaaa
    18181 aatgtcggta gcttctttag tggttggcat atttgctcct tcaccaagta aagccaaccc
    18241 atttctaaca aggttagagg catcggtcgc attgatctca ttctgagttg cacatgggaa
    18301 tgcgcagtca gctttaatat cccataaagg atttgagccg gcatcgcctg gcactgcggt
    18361 gaaagtcgcg cttttgtact tatcggcata ttcgctgatt ctacctcgtt tcacattctt
    18421 caattcctta ataaagtcaa gcttttcctg atttattccc gcttcatcat aaataaaacc
    18481 tgaagaatct gatgaagata ccggtttacc gcccaactga attattttct gtatggcaaa
    18541 ttgagccacg ttccccgaac ctgaaaccaa acaagttttc ccttttatat ctttgccaat
    18601 gtttttaagc atattttgag cgaagtaaac caggccatat cccgtggctt ccggtctgat
    18661 aaggctgcca ccccagccgg cacctttacc ggtaagaact ccggtaaatt gattttcaat
    18721 tttcttatat gctccaaata aatacccgat ttctcttcct cctactccga tgtcaccagc
    18781 cggaacgtct gtccggtgac caatatgtct gcttaactct cccatgaagg cctgacaaaa
    18841 acgcattacc tctgcctccg attttccttt gggatcgaaa tcagaccctc ccttgccacc
    18901 gcccataggc aggcctgtca acgcattttt gaacacttgt tcaaaagcga gaaattttaa
    18961 gatgctctga ttaacagaag gatgaaaccg taatccacct ttgtagggac caatagcgct
    19021 gttcatttga atcctaaatc cacggttgat ctgaatttca tttttgtcat ttgtccatgt
    19081 aaccctaaag gtgattaccc gctcgggctc aatcattcgt tccatgattt ttgcatcccg
    19141 gtatttcttt tctttttcca gtactggacc tatagttaaa aatacttcct caactgcctg
    19201 atgaaattct ttttcgccag ggcttctttt caagaggtcg tttagaatgc ttgattcttg
    19261 cattttggtg ggttttttag ttggctgtaa agataatatt tggtatacga tattagaatt
    19321 tcactatgaa atttatttta agggagatta tcaaagttta gactttaaat accgggcggt
    19381 gtagcttttg gtcactttga ttaagccttc cggggttcct gagtagatta tattcccacc
    19441 aagttctccg ccttcagggc ctaaatcaat aacccaatcc gaacatttta ttacctccac
    19501 gttatgctca ataattacaa ccgaattgcc ctggtctaca agagcctgga tcgcagtgag
    19561 aagtttatgg atatcatgaa agtgcaggcc ggttgtaggt tcatcaaaaa tgaatagtac
    19621 gtgattgccg ggatcggttt tgccaagaaa tgaagccagt tttaccctct gggcctcacc
    19681 gccacttaaa ctattggaag attgtcctaa ccgtacatag cccagtccta ctttttgaag
    19741 tggcaataat ttagaataca ctgaatttaa ctcttcaaaa aataccaaag cttcgtcaac
    19801 agttagatcg agaatttccg cgatgttttt atcattatag gtcacttcca gaatttcaga
    19861 tttgaatctc ttgccatgac aactttcaca agtgagaaaa atgtcggcca taaattgcat
    19921 ttcaatcttt tgtgtcccct ctccctgaca agcatcgcat ctacctccat ccacattaaa
    19981 tgaaaagtgt ttgggctgat aaccgcgctg ccttgataag gactggtcgg ccattaattg
    20041 tcttatgccg tcatatgctt ttacgtatgt tacgggatta gaccgtgatg atttacctat
    20101 tggatgttgg tccacaaatt ctatctgggt gattttcttg taatctcctt ccagtttatc
    20161 gtactttccg gtttgttccg ccaccgttcc caaaatttta ccaattgatg gatacaggat
    20221 attttttacc agtgtagatt ttcctgatcc gctaacacct gttatagtgg ttaatactcc
    20281 cagtggaatt tctacatcaa tatttttaag attattctcc cgtgcgcctt tcagcacaat
    20341 tgatttcgac cattttcgtc tgtttttcgg aacttttatt ttatcaatac cattcagata
    20401 acgcaaagta tgcgttaatg gatggccatt taattcttcc agtttaccct gaaaaactaa
    20461 ttggccgccc aaccgtccgg cctcagggcc aatgtcaata atctgatctg cagcaagcat
    20521 aatttcctct tcatgttcta ccacaatcac agaattaccc aggtcgcgaa ggtttttcag
    20581 cacttcgatg agtctgccgg tatcccgtgg atgcagcccg atgctgggtt catccagaat
    20641 atacatggaa cccaccaagg cgcttcccag tgaagttgcc aacttgattc gttgaaattc
    20701 tccaccggaa agggtgtttg tcagccgatt caacgtcagg tattcaagtc caactttttg
    20761 caggtattcc agcctgcttt taatttcccg gatcaccctg ccggcaactt tctgctgaaa
    20821 tttgttcagc ttcatttttt caaaaaagga agtaagttca ttaaccggca ataataccag
    20881 gtcagtgatt gactgatttt gtattttaac atacgaagca tctttcctta gtctggttcc
    20941 cctgcaatcc gggcaagtag ttctacccct gtaacgggat agcaataccc ggtattgtat
    21001 cttgtgactt ttcgtccgta cgtaattaaa gaaagcatca attcctttga aatgcttatt
    21061 gcctctccaa agcagatcga tttcctttct tgataattca gaaaaaggcc ggtgtattgg
    21121 aaaatcaaac tccgcagctt tttttaccag gggcgtaagc cacttcttca ttttttgact
    21181 gcgccaggga gctatggcgc cttcataaag ggaaaggctt ttatccggga ttaccagatc
    21241 ttcatcaatt cccagaattt ttccaaaacc ttcgcatgtt ctgcaggcac caaagggatt
    21301 attgaagctg aaaaagttga ccgagggaat ttcaaattgc atcccatctc gttcaaattt
    21361 atccgagaat gatttagaca tcctcccttc catttctact ctgcattcgc catgtccttc
    21421 aaagaatgcg gtttgtacag agtctgaaat tctaaactga ctgtcctcgt cattttttac
    21481 cacaccccga tcaatcaaaa tattaatctc cctggttcgg ctgtttttct taacttttat
    21541 taaatcttct ataaagacca gctctccttc aataacaact ctggaatatc ctttgcttaa
    21601 tagcaatttt aattcattgt caagagtccg gttgtcgggg attttcagcg gacatgaaat
    21661 cgtataccgt gtatttgttt cctgggaaga aatgaaatcg acaacatcag aaacggtatc
    21721 acgttttact tctttcccac taacaggact tattgtagca cctactctgg caaacagcaa
    21781 ttttaagtaa tcataaattt cggtggatgt gccgacagtt gaacggggat ttctggtatt
    21841 caccttttgt tcaatggcaa tagccggact gactccgcgg atgtaatcta cctcgggttt
    21901 ttccatgcgg ccaagaaatt gccgcgcata ggaactaaga ctttccacat acattcgctg
    21961 tccttcggca aaaagcgtat cgaatgccag tgacgatttc ccggagccag aaagtccggt
    22021 aataataacc agttgattgc gtggaatggc gacgctcagg tttttaagat tatttacgcg
    22081 agctcctttt attatgatga attttttcgg atcaagtgaa tccaaaggac ttttttctgt
    22141 caaaacaccc gttttcttca tcagggtgca aagatacgaa accaattagc aattagttat
    22201 actgcaatta gattaagatg gaatctcatg aggccagtaa agcctttccg gaagtgttaa
    22261 catgccggcg gtgtccgctg atgccgcaga tcattgcaga gaagaaattg ctcagggagt
    22321 cttctgcgca aatccgcata atctgcggga gaaaagaagt tttctgcgca aataagttag
    22381 ttgatctttg ggaggggagt tatttttcca atggcggatg ctggaactga ctccagaatt
    22441 ctttttcggt acagtagggt gtttcagaca gtcttataaa tgaacgtcaa aatcgaattt
    22501 agaattttga atctgtttct ctaatcaatt caaatttacc gttgtaaagc attttccaat
    22561 cattccggta ataagaataa accgtgtcta atttttcatt aactccatca aaactttcat
    22621 aaacaacagt atccttaaac cgatagttta ttgaattatc actcttaatt atgaccaact
    22681 cttcttcatg tttcgaattt aaatttaaat gtttattcat tttaaaacag tgtttctgga
    22741 ccattcttgc tggtttgaat tgtctgtcca tgaataaggt tcgtgccatt atcattgagt
    22801 ttaaatgtta aacaaaattt ttctctcagg aattctacgc ggaccgaatg tttagtttag
    22861 tgtgtcatag taattcgttt ttttggtcaa ttgttatttt tcattttcaa ttttcagaat
    22921 caggttagag cgatatctaa tcttaattgg taagttgaat tttattattc ccccggattc
    22981 ctaccaggta acaatttttt attttttaac ctttggatca aagaattatc tctattctta
    23041 ttttgttcct tatcagataa accaaacctg cctccatctg ggtttgggga aaggaaataa
    23101 taaataccct cttttgtggg ttcctcaaaa taaaagtcga tattgttgga accggaaatt
    23161 tgatcatagt cttcgacatc cttgaggtcg acagccgaca gataatatgg ctcaagcatg
    23221 gaaagattaa gacttgggta agtattcatt attggggcag gaataggaaa aactttgggt
    23281 tcatcttcag gacctccaat agtataaaag gtgactacgt atggttcatt tgaatgatcg
    23341 taataagacc aatcagccat ataatagtca aaatctccgg tatgtgttaa tgtaaaacca
    23401 gaattgtcgg ccgatgtaat attcacgtca aattccgggt cctccagagt agtcaacagg
    23461 tcgcccttct taataacgta aacctctttt gaatctgagc caaatccaat gatagtggta
    23521 taggattcaa aaccgggaat ataaaagtat tcctgttgct cagtgtaaga taaaagataa
    23581 gatcgggttt catcattaac ggaaaggttg ccgtatatat aattatcaaa aatgtttttt
    23641 tgtgtggcta cttcattctt agacatttct tctgtttggg ttagatcgat gttgatgttt
    23701 cccgcctctg cattttcgat ccacttatac gaagcagttt ggccggatat cagcccgata
    23761 aatatattgg ccgggttcca ttttgtaaat aacgggtaat tgaacagggg tgaaaaaacc
    23821 tgactcttaa atccattact aacaattgca agatcgtggg ctgggatatt ggagaaagtt
    23881 aaattctgat tatagccctc tgtttcattg taagatggga ggatccaatt gctgctcttg
    23941 catcgttgta cggttattgc ataaaaaaac ttgttgttac cataatgaga agccacaaag
    24001 atggtggcat taaaatccca gccatcataa ttatttggat aaataaaatc ctttgttgaa
    24061 ttattattca gttcatgata ctcaaccgct tcgccgtcca tatcataaat tattatataa
    24121 ccagtactgt tttcttcgaa ataattctct gcttgcacgg tcaacattaa gtttcggata
    24181 ttaaaactaa cgttagcaaa agcagaatta ccttcactat ctgaaacgat ggctttgatt
    24241 gcatgttgtc cgtcctcgta atctccggta ttgatttcaa actgataagg atcattgcct
    24301 gaaattgttt caatcatcac atcatcaatt aaaaaatcaa tcttatggac ttcacgatca
    24361 tccgttgcgt taaattcaag tgtaaaatct ccccttattt gctgattatc atccaggttg
    24421 gtaaaagaga ttataggatc agtagtgtct tttgatttgt cctggcatga agaaaaaata
    24481 aaaaaaatgc aggtaatgag tagagtgatt tcgttaagag taattttttt catttgaaat
    24541 aaaatttaaa aaggttaaac aaaaattttc tctcaggaat tctacgcgga ccgaatgttt
    24601 agtttagtgt gtcatagtaa ttcgtttttt ttggttaact gttatttttc attctcaatt
    24661 tttaaaatca ggttagagcg atatttatct gaattagtaa gttgaatttt ttcgttcccg
    24721 gtgttttggg aaagtaaaaa ccgataccat tcgaattggc tgaaaaaatc tgccagaacc
    24781 tgatcggtga atcgccatcc tcttcgggcg aagactgcgt ttctgttgaa accttacttc
    24841 tttctaacca acgcattaat aatagccagg ttttctacat cggttttagt taagttattg
    24901 tttacttcct tggttcggga tatcaatttc tccggttcat tataaaaagc aaaaagattg
    24961 aaatatgctt gatagaattc attatcaaaa gcataattgt atttggcgaa aatcagattt
    25021 ttcaaaatgt taagttgatc cgaattaaaa ttattcaatt ctcttttggt tacagtatga
    25081 cctttgaaca gctgataagt gattttttca aagtctttat ttattaaata gtttacaata
    25141 actttttcct tattgtcaag tattgatctg agataaaaaa actcgttatg gccttcctgg
    25201 atattctctc ctatgacctt ccagtgctca tgttttttaa ggatgtgggt tgtatcattc
    25261 aggtctttgg aaaaaatcag gtctgtattt tcaggcaaat tgccgtaatc cactgcggcc
    25321 atgagaaagt cgtaatcata atgcgagaag tagaagttaa cgcggaagtc ttcatattga
    25381 ggttcaaatt gatttttata aggttgtcgc aattgatcga agttaatttc ttcaactgaa
    25441 tcctttctta taataaaata tctatacaag gtgcatccgc ctccccaagc acatgactca
    25501 caaaggattt tttcctttga aagttcatga attttccaaa tctcttctaa tgacctgtat
    25561 tcttctttat aatctaaata agggtttaag tctgcaaata ctgaaacatt cccgtcttct
    25621 gccaattcta ataactggtc tgatttatta cttccaaagt ggaaagttct tggtgtaaaa
    25681 gaacgtgact cgagtagttt gtttaatcct ctttttccaa tgttaagaga aaaaactctg
    25741 tatattttat caatatagtc gaattgaaat cctcgaaaag tgtccttaaa ataatgaata
    25801 tccttatcaa aattaattcc agagctatcg aaaagcgttg catttaggta gattagtcga
    25861 tctctttcgt ttttctttcg aggtgcataa taattaattc taacctgatg taaagtatca
    25921 atatcctgta caatcaaata cttaagtgta tcactatcct taataatatt attttgagca
    25981 aaaagagctt tactaagcag caatatgagg ataagaaaga aagaagaata tttcatatga
    26041 ctttttttaa aaatcctaga tataaattaa tagaggcatc tacctcatca ccgaatcctt
    26101 cctcaaccat taagatgccg gtagttctac ccgcctgaat gatttcctta ttttcagcat
    26161 tactcatcca acccatggca atatgacctg ctgcagggtt ttgatttatt gttatataaa
    26221 ccataaaccc attatcaaca tacgtattaa tttctgattt aggtatccca ataaagtttt
    26281 gactagaaac aatataatca tgaatcttat ttgcaactgt ccccgtcgcc cagggcaaat
    26341 ggcctaggac ataatagctt aggtcttgcg caaagaaatt gcaatatgta gcgcgccaac
    26401 catgtatttg aagccaaccc atggttggta aaagttcacc aatatcttta gtatttgaaa
    26461 ttgtattcaa tacagaccat ggattatcct ttttcactgt gaaataacca gtaggtgtta
    26521 cggcgtttaa ttggtactta ttttcattat tatcataaat ccaaggccaa ttggacgcta
    26581 tttcattttc atgagtataa ccccaatcaa ctatttcatt aactactgaa gttaaattat
    26641 ctggtcgcga ctcaggcaag ccatgaaaag taatagcatc tttaggttgc gttggcccca
    26701 atacactttt ttcttcagga gtaaattcat aatagtgaca gtctccatat acgcaatgcg
    26761 ccctatgata tcggtcatcg gtatttaaca tttcattccc tttttgctct aatctctcac
    26821 gatatgtcgg gttcaacgcc tctccaatgg cgtacaggcc gctgctaatt gtttctgtat
    26881 agctattttc ttttttcatg atttcgtccc tgatatacct tcttactaaa tccggtatcc
    26941 cttcatccat tagcatatca ctaaaattca aagcatccag tacataattc ccattcccct
    27001 ggtcaagaaa aagcattaag acagcaggtt gagaaaaata cctgagagac caatttatat
    27061 aagtccccaa actaaacaaa gtcagcccac tctcatccct tcctgtaact cccccttcct
    27121 caaattcatc ttcactcata aaatctattt ggattattaa atcctgccca ttcactttgc
    27181 ggtttgctga taaagtcctt ccttcagctt caattctatt ccgttcctca gtgcttaact
    27241 tacccagcgg atcataaacg gatggtttat tcattgctcc taaatatgca gcttggcaaa
    27301 aaaagagaca tattaaaagt attttattct tcattttcgt taattctttg attaatcact
    27361 ccttcaatgt atttgatttt ctcgggtgaa ctttccggaa gcccactatc attcctgatg
    27421 ctttccacaa gttcttcaaa aggttcgcct tctttgagct catttaataa ctcaatcgtt
    27481 ggtttcaact cttgaatagg gatatctagt tgaatgagat gattcctggc ttcatctatt
    27541 gattttaccg attggctcaa tcgtgacctt atattttctg gtttatccac tattgcgata
    27601 tgaaggctaa tcccttcccc gataagctcg tcattttcac ctcttatcct gggtggatag
    27661 aaattctcac tttcaattat gccttcaatc gcttctataa atggctgcat ttgtcctttt
    27721 atgctgtcaa tagtggcttc acttgcttct atttggtgaa ttattgctgc atataccaag
    27781 ctatctaaac cagttaaatt tctctcttct tccgtattcc caaccggtgt atttccttca
    27841 cctgagactc cttttttcac ctccccacct tccaccgtat aactattccc agcactatca
    27901 gtaatggtta cagctttatc ttctccattc tcatctttgg gaatttcatg cgtcgttgca
    27961 ttgccggcgg tgtccagcac tacgattttt ccgtcatcgt tgatgaagac agagtcaatt
    28021 tcattttcga tggtgatttc ggtggaagcg atcgcggttg ttgttgctga ggatgttgat
    28081 gttgttgtcg tagtttccgt agtctccgtc ctgtcttttc tgccgtcata cacgaacgga
    28141 ctgtcagggt tatgaaccgt tactatttct ccgtgggtca tctggtaatc ttcatt
//
LOCUS       DASWHX010000170         5383 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_918532, whole genome shotgun sequence.
ACCESSION   DASWHX010000170 DASWHX010000000
VERSION     DASWHX010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 5383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5383
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_918532"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..838
                     /locus_tag="VGA21_11295"
     CDS             <1..838
                     /locus_tag="VGA21_11295"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HEX9651138.1"
                     /translation="NCTLTIESGSAIYFHNNSSLFVAGTLSVEGSNDQRILFTNDRLD
                     EPYRSTPGQWNGIYFLEGSANNKIDYAIIKNATYGIWLGTPDDDTTPDLEISNSIIEN
                     ISNTGLIAFTSDLLAYNLLINNCGEFTLANLAGGNYDYTHLTLANFGFSFFRNSPSVA
                     FSDNLDLSDGTQIKADLNVSMVNSIIWGNLQEELLFNFQSSTSITSFHHNLVKTQLDT
                     FAINNLINTDPLFINPEIFNYKIDSLSPAINAGVPTFVTFDLDGRLRNGSPDIGAYEI
                     KN"
     gene            complement(848..1318)
                     /locus_tag="VGA21_11300"
     CDS             complement(848..1318)
                     /locus_tag="VGA21_11300"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEX9651139.1"
                     /translation="MEDKSKYASFLKRFFAYVIDLILVGFVQSLVVMPLLGLLGFGIA
                     VGKDFSEPEKLTFIGILAGLGIAAYIGIFIIGWLYFALLESGSRQATLGKMAMDIKVT
                     DIYGDRLSFVKATLRYFGKYLSGLFFCLGYIIAAFTEKRQALHDFIANSLVVNK"
     gene            complement(1320..1586)
                     /locus_tag="VGA21_11305"
     CDS             complement(1320..1586)
                     /locus_tag="VGA21_11305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013453639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651140.1"
                     /translation="METPFEQGQISGEKTIPVSLWDWIITIFITMIPVLGQVMLFVWA
                     FKSDVNPTKANWAKANLIWFGIALILFTLFAIIFGIAAFSFMNT"
     gene            complement(1641..2894)
                     /locus_tag="VGA21_11310"
     CDS             complement(1641..2894)
                     /locus_tag="VGA21_11310"
                     /inference="COORDINATES: protein motif:HMM:NF025034.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl protease family protein"
                     /protein_id="HEX9651141.1"
                     /translation="MNNRREIFLRKVILIFAFNLVISLSCLAQSRLGFQLKDGQKKAV
                     IPFELHNNLIVVPVILNDALPLKFILDTGVRTAILTERTISDIINLPYSRSLSIRGVG
                     GEKIVDAQIATNVTLELPGVRGKGHALFVLEEDYLKLRNYLGTEIHGILGYELLSRFI
                     VKINFNRKKITIYDREYFDSPSRYEKLDITIENTKPYLVTPISINSNQSLNAKLMIDT
                     GSSHTLMLLSNDNVNFEIPEKNVESSLGSGLGGLIYGKVARIHNIQFGEINIKEPVVT
                     FPNRESYPDSLRFSYRNGTIGGGLLAQFNLIFDYQSNSLYIKKNKAYGKAPHYNLSGL
                     TLIADGVHLDEFRIVEVRMESPAEKAGLLVGDKILTVNGKTTEGMEFDSLLGFFNSKV
                     DRLIRIEYSRDDRTYFTSFRLRKIL"
     gene            complement(2970..3944)
                     /locus_tag="VGA21_11315"
     CDS             complement(2970..3944)
                     /locus_tag="VGA21_11315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651142.1"
                     /translation="MLKNYFIAAIRAIRRSPWEAMLKIFAIAAGVCASVIHLAFFSSG
                     DFSDFHELWPSLVLIFLGAALLEQFYGQPQNTGEYKVRIILGDEDLLMRGRKITEAAL
                     VIFAGVILGLVISDSYFTYFHKTNLLIPEGQYMPGILILGFFHFTFFLFTVWFGSAIG
                     VKSDSIRFSTFSKTLAGGLIFVTSLFAIFWSVTSQETSIGFRIYLFDIVALSCFSYAS
                     WNKLAQYTLESKVRQFSTMKLQGASTLRMLFYWIQQLGPSALISVGLSILIGNLYLIL
                     EMPFKNSALSALITFSWTAFITVIIILGLSQAIARGFKQETNGFINHE"
     gene            complement(4088..4669)
                     /locus_tag="VGA21_11320"
     CDS             complement(4088..4669)
                     /locus_tag="VGA21_11320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013453999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEX9651143.1"
                     /translation="MKKICVFCGSNSGFSEVYRKSTIELAELIVERQLEIVYGGGNVG
                     LMGILADHVLQGGGLITGVIPHFLAAKEVGHDRLTKMIFVDSMHERKQKMSELADGFI
                     ALPGGFGTLEELAEILTWAQLGLIQKPVGLLNVDKFYSPLILQLDMMVEKGFLKTENR
                     QLLLADATPSGLLLKMDNFIPVKTTKWISKDET"
     gene            4719..5105
                     /locus_tag="VGA21_11325"
     CDS             4719..5105
                     /locus_tag="VGA21_11325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017733535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX9651144.1"
                     /translation="MKKRVNISSGAEWEEIVGYSRAVRIGNQIEVSGTVSLKDGVPFA
                     NGNAYKQTVRILEIIRDAIEKAGGKLENVIRTRIFVTDISKWEEIGRAHGEFFSTIKP
                     ATSMVEVSKLIGDDYLLEIEATAVLT"
     gene            complement(5102..>5383)
                     /locus_tag="VGA21_11330"
     CDS             complement(5102..>5383)
                     /locus_tag="VGA21_11330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013453585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pseudouridine synthase"
                     /protein_id="HEX9651145.1"
                     /translation="GRTHQIRAHLKHIGHPLFNDVTYGGDKILKGSQFSKYNSFVKNS
                     FKLMARQALHAKSLGFVHPAKNTFVQFDSELPEDFVAVLEKWREYVKYN"
BASE COUNT         1748 a         1103 c         1001 g         1531 t
ORIGIN      
        1 taactgcaca ttgacgatag aaagcggcag cgcaatttat tttcataata attcttcgct
       61 ctttgtagcc ggtacattat ctgttgaagg cagtaatgac cagcggattc tttttaccaa
      121 tgaccggctg gatgaacctt atcgctcaac tcccggccaa tggaatggaa tttacttttt
      181 ggagggaagc gcgaataaca agatcgatta cgcgataata aaaaatgcta cctatggaat
      241 ctggctggga actccggatg atgatacaac cccggatttg gaaatttcaa attcaattat
      301 tgaaaatatt tccaacaccg ggttaatcgc ttttacttct gatttattag catacaactt
      361 gttaattaat aactgcggag aattcaccct ggcaaatttg gccggaggca attatgatta
      421 tactcatcta actcttgcca atttcggatt tagttttttt cgaaattcac catcagttgc
      481 tttctcagat aatctggact tgtcagatgg tacccagatt aaagcagatc tgaatgtatc
      541 aatggtcaat tctatcattt ggggaaatct tcaggaagag ttgctgttca attttcaatc
      601 ttcaacttca ataacttcct tccatcataa tctggtaaaa acacagctcg acacttttgc
      661 aattaacaac ttaattaata ctgatccact ttttattaat cctgaaattt ttaattataa
      721 aattgattcg ttatcgccag ccataaatgc cggtgtgcca acgtttgtta catttgattt
      781 agatggcagg cttcgcaatg ggagtcctga tattggcgct tatgaaataa agaactgatg
      841 aaattcatta cttatttaca accagggaat tcgcgataaa atcatgtaac gcctgtctct
      901 tctcggtaaa agcggcaatg atataaccta aacagaaaaa gagtcctgac agatacttgc
      961 caaaatatcg caaagtagct tttacaaatg aaagccggtc tccataaata tccgtaacct
     1021 taatatccat agccatttta ccaagagtag cctgtctgct gccggattcc aataacgcga
     1081 agtaaagcca gccaataata aaaattccga tgtaagcagc aattcccaat cctgccagaa
     1141 taccaataaa agttaacttt tcaggctccg aaaaatcctt accaacagca atgccaaatc
     1201 ccagtaatcc caacaaaggc ataacgacaa ggctctgaac aaatccaaca aggatcagat
     1261 caattacata agcaaaaaat cgtttcaaaa aactggcgta tttactttta tcttccatgt
     1321 taagtgttca taaatgaaaa tgccgcaatc ccaaaaatta tggcaaataa ggtaaacaaa
     1381 ataagggcaa ttccaaacca aatcaaattc gcttttgccc agtttgcctt tgtcgggttc
     1441 acatcagact taaatgccca aacaaaaagc attacctgtc ctaaaaccgg aatcatggtg
     1501 atgaagatgg taattatcca atcccatagt gagacaggaa tggttttttc acccgaaatt
     1561 tggccttgtt caaaaggtgt ttccatacaa attgagaaat tattacaaag taggcaatca
     1621 gtaattaaag gtaaaatttc ctacaatatt tttcgaagtc gaaaactggt aaaataggtg
     1681 cgatcatcgc gtgagtactc aatccttatt aaccggtcaa cctttgaatt aaagaatcca
     1741 agcaaactat caaattccat cccttcggtt gttttcccat ttaccgttag aattttatca
     1801 cccaccagta agcctgcttt ttcagccggg gattccatcc gaacttcaac aattctaaat
     1861 tcatccaaat gaactccgtc tgcaataaga gttaatccgc ttaaattgta gtgaggtgct
     1921 ttgccatagg ccttgttctt ctttatataa agtgagttgg attggtaatc aaatattaag
     1981 ttgaactggg caagcaaacc accgccaata gttccgtttc tgtaagaaaa tcttagcgag
     2041 tctggatagc tttcccggtt gggaaacgtg accaccggct cttttatgtt aatttcacca
     2101 aactgaatat tatgtatacg agctactttt ccatagatta atccgcccaa gccggaaccc
     2161 agactacttt ccacattttt ttccggaatt tcaaaattca cattgtcatt acttaatagc
     2221 attagagtat gacttgaccc ggtatcaatc attagcttcg cgttgaggct ctggttggaa
     2281 ttaattgaaa tcggggtaac caggtaaggt ttagtgttct caatagtaat gtcaagtttt
     2341 tcataccttg agggagaatc aaaatattcc ctatcataga tcgttatttt ttttctattg
     2401 aaatttattt taacgataaa tctgctcagc aattcatatc ccaggatgcc gtgaatttct
     2461 gtgcccaggt aatttcttaa tttcagataa tcttcttcaa gaacgaataa agcgtggcct
     2521 tttccgcgga cgcctggaag ctcaagcgta acgttggtag caatttgagc atcaacgatt
     2581 ttttcacccc caactcctct aattgaaagg gatcgcgaat aaggaagatt gataatgtcg
     2641 gaaattgtcc gctccgtgag gatggcagtt ctcacgccgg tatccagaat gaatttcaga
     2701 ggaagggcat catttaaaat tacaggaaca acaatcagat tgttatgaag ctcaaacggg
     2761 attacagctt ttttctgccc atctttcaat tggaatccca acctgctttg agccaggcaa
     2821 ctcagagaaa ttaccaggtt aaatgcaaaa atcaggatta cctttctcag aaaaatttca
     2881 cgtcgattat tcactttaga tgcctgccaa ttgtatgggt aaattacaat aagcaatcat
     2941 tacaaaaaag aagccaaaat gattattccc tattcatggt ttataaaacc attcgtttcc
     3001 tgcttaaaac ccctcgcgat tgcttgtgaa aggcctaata taataataac agttatgaat
     3061 gcggtccagc taaaagtaat taaagcggag agtgcggaat ttttaaaagg catttcaaga
     3121 atgaggtaga ggttgccaat gagaatggag aggccaacag aaatcagcgc cgatggtcca
     3181 agctgctgaa tccagtaaaa caacatcctg agggtagatg caccctgaag tttcatcgtt
     3241 gaaaattgtc tgacttttga ctccagggta tattgtgcaa gtttgttcca ggaagcgtaa
     3301 gagaaacaag acaaggccac tatatcaaaa agataaatcc tgaaaccaat ggaagtttct
     3361 tgtgaagtaa ccgaccaaaa aattgcgaaa agagaggtaa caaagattag tccacctgcc
     3421 agtgttttac tgaatgttga aaatcgaatc gaatcactct taactccgat ggcagatcca
     3481 aaccaaacgg taaaaagaaa aaatgtgaag tgaaaaaacc caagtattaa tatgccaggc
     3541 atatactgtc cttcaggaat taacaaattt gtcttatgga aataggtgaa gtaagaatca
     3601 ctaattacca aacccaaaat tactcctgca aaaattacaa gggcagcttc cgtaattttc
     3661 cgacctcgca tcagcaaatc ttcatcgccc agtattattc ttaccttata ctctccggtg
     3721 ttttggggtt gcccgtagaa ttgttccagc agtgcggcac caagaaaaat gagtacaagg
     3781 cttggccata attcatggaa atcagagaag tctcctgaag aaaaaaatgc caagtgaatt
     3841 acagaggcac aaaccccggc ggcaattgca aatatcttta gcattgcttc ccagggactt
     3901 ctcctgattg ccctaattgc agcgataaaa tagtttttta acactttttc tgattgagca
     3961 gcataaagaa atcaagaagt atacctcaaa atgaaaagtt ctggaatgcc tcaggaggcc
     4021 attttttgga aagaatccct ggcttaatgt tcaaaggtga gacagaattt cgttcagatg
     4081 tgaacaatca ggtttcatct ttggaaatcc atttggtagt tttaacggga atgaaattat
     4141 ccattttcaa caagagacca gatggcgttg catcagcaag caaaagctgc ctgttctcag
     4201 ttttcagaaa acctttctcc accatcatat ccagttgtaa aatcaatggc gaataaaatt
     4261 tatcgacatt caaaagtccg acaggtttct gaatcagccc taattgagcc caggtcagaa
     4321 tttcagccag ctcttccaga gttccaaacc cccctggtaa agcaataaat ccatcagcca
     4381 gctcactcat tttttgtttt ctttcatgca tgctgtccac aaaaatcatt tttgtcaatc
     4441 tatcgtgacc gacttctttc gcagcaagaa agtgcgggat aaccccggta atgagtccgc
     4501 ctccctggag gacatgatca gcaaggatcc ccatcaaacc aacgttacca ccaccataca
     4561 cgatttccaa ttgccgctct acaataagct ctgccagctc aatagttgat ttccggtata
     4621 cttcagagaa acctgaattg gagccgcaaa aaacacaaat ctttttcatt taatggaatg
     4681 aatgtcaatt actttgcaac attaaatcac aatcaaatat gaaaaaaaga gttaatatat
     4741 catcgggagc tgaatgggaa gaaattgtgg gatactccag ggcggttcga attggcaatc
     4801 aaattgaagt ttcaggaacc gtttctttaa aagatggagt tccattcgcg aacggaaatg
     4861 cctataaaca aaccgtcagg attcttgaaa taattagaga tgccattgaa aaagcagggg
     4921 gtaagctgga gaatgtaata aggacccgga tttttgtaac agatatttcc aaatgggaag
     4981 aaatcggccg ggcgcacgga gaatttttca gtacgattaa accggcaaca agcatggtgg
     5041 aggtaagcaa acttatagga gatgattacc tgcttgaaat agaagcgact gcagtactta
     5101 cttaattgta ttttacatac tctcgccatt tttcaaggac agccacaaaa tcttccggca
     5161 gttctgagtc gaactgaaca aatgtatttt ttgcaggatg aacaaaaccc agcgattttg
     5221 catgtaaagc ctgtcgtgcc atgagtttga aactgttctt aacaaaggag ttgtattttg
     5281 aaaattgact ccctttcaaa attttatcac ccccataagt cacatcatta aatagcggat
     5341 gcccgatatg ttttagatgc gcgcggatct ggtgggttct ccc
//
LOCUS       DASWHX010000171        12734 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_2046090, whole genome shotgun sequence.
ACCESSION   DASWHX010000171 DASWHX010000000
VERSION     DASWHX010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 12734)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12734)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12734
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2046090"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1151)
                     /locus_tag="VGA21_11335"
     CDS             complement(<1..1151)
                     /locus_tag="VGA21_11335"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX9651146.1"
                     /translation="MGAIKNFLVLFILFVSTTTSAQINRYVIHFTDKNNSEFTINNPS
                     AFLSERSLTRRIKNNVSVTEQDFPVTKLYVNELENEGVNVFYTSRWFNVAIVEMDVSL
                     ITIIEDLAFVSEVEYVAPGTKLNGRSVDYESKNTKNRTTAGESDFQINYMGADEMHDD
                     GFKGNGILIAVTDAGFTNLNNISQFTHLYTNGKILYTFDYTVNDINVYLHDDHGTEVF
                     STMGAFDSGFTGSAPEADYMLFVTEDINSEFRIEEYNWLLAAEFADSAGVDIINASIG
                     YNTFTDASMNYTYEDLDGNTTVISRAAAIAASKGILVVTGSGNEGNTNWRNVLAPGDV
                     ETVLTVGSVTGNGNISSFSGVGPTSDGRIKPDLVALGSNVAVINETGNI"
     gene            complement(1202..1966)
                     /locus_tag="VGA21_11340"
     CDS             complement(1202..1966)
                     /locus_tag="VGA21_11340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651147.1"
                     /translation="MLKKLLWISYLLIIFISCQEPTFVNSRGSLDWDFLPLEVGVIST
                     YELNRVSYLGRLIEPEVEQFLLREEIVEKQVFADGSSLFTILESIPDLNGDWIPKKYF
                     TIRHEFGSAISVIDNIETVELQFPLLNGFRWEGRYSLNDCPDGGEYCDYFEVADRDKE
                     FTAGGITYPKTARIIRQNTQDPLQITEDLVEFAVYAQNIGLIYSETNQLEYASCRNNQ
                     NADCCCPDNILCTLCAGEIEFGFMEKKTLIERITPN"
     gene            complement(1941..3068)
                     /gene="mnmA"
                     /locus_tag="VGA21_11345"
     CDS             complement(1941..3068)
                     /gene="mnmA"
                     /locus_tag="VGA21_11345"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002694600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEX9651148.1"
                     /translation="MSKKGRVLVAMSGGIDSSLAAILLHEQGYEVIGMTMKTWDYAGS
                     GGTKKETGCCSLDSINDARNIAVSLGFPHYILDIRNEFGDFVIDHFTSEYIAGRTPNP
                     CVLCNTHIKWDALLRRADKLNCEFIATGHYANVREENGRYIISRGIDLNKDQSYALWG
                     VSQESLSRTILPLGKITKPQIRDMANERGFGELAKKPESYEICFVPDNDYRGFLKRRV
                     EGLEAEVKGGEFVLEDGTVVGTHEGYPFYTIGQRKGLKIALGYPVYVTEIQKEKNRVV
                     LGTFDELSRDGMYVKKLTLNKYPNLYGKRLDTITKVRYNDHGTPAVIEQAGDTIKVYF
                     GKGVHAIAPGQAAVFYEDDDVIGGGWIQSSFKQHAQKIIMD"
     gene            3206..3808
                     /locus_tag="VGA21_11350"
     CDS             3206..3808
                     /locus_tag="VGA21_11350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651149.1"
                     /translation="MGLKTLVKLSQVTNLSDARYAAGMGVQMVGFNINPESDFYIDPD
                     KFKGIIAWISGVTLIGEFEGSVFSYNAGYNFDYIQISNPDFVKAITGLKKKLIFQIDL
                     DIYDEVAAEKIMTELKDKVEYFLVTKSDEKLNGKEIKSWARLFPIILGFGLQEETIEQ
                     VLQEYPIKGISLIGGEEKVVGFKDYDQLAGILERLQTEKG"
     gene            3893..5635
                     /locus_tag="VGA21_11355"
     CDS             3893..5635
                     /locus_tag="VGA21_11355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015028722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEX9651150.1"
                     /translation="MKDFQIKQSAKEYDVVIVGSGAGGGMAAKVLTEAGAKVAVLEAG
                     PNFNSETDGNMFTWSYDSPRRGAGSREKPFGEFDACYGGWYLEGEPYTTAPGTQFNWW
                     RARMLGGRTNHWGRISLRFGPLDFKRKSLDGLGDDWPISYDDIKPYYDRVDKFVGIFG
                     TKEGIYNEPDGIFMPPPKPRGYELLVKKACDEIGVPIIPSRLSIITQPLNGRAPCHYC
                     GQCGRSCATHSNFSSPSVLFPPAHATGNLDLITGAMAREVVANSEGKCVGLSYINTLT
                     GEEYFIKGKVVILAASACESARLLLNSKSPAHPNGLANSSDVVGRYLTDSTGASAGAL
                     IPALVDGLPHNEDGVGGMHLYVPWWLNDKKLEFPRGYHIEIWGGRGMPGYGFGGGIQN
                     QNNYLGIIDESPREKGGGGYGLTLKNDYRKLYGAVVGFSGRGEAIAMRDNRCEIDPNV
                     VDKYGIPVLRFNYTWTEHEVNQARHMQDTFEDIIDAMGGISLWGKPGADRQYGLTKPG
                     EIIHELGVTRMGNDPKNSVLNKYCQAHDVKNLFVADGGPFVSQADKNPTWTILALSWR
                     TSDYIIQEMKANNL"
     gene            5638..6252
                     /locus_tag="VGA21_11360"
     CDS             5638..6252
                     /locus_tag="VGA21_11360"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020529027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 2-dehydrogenase subunit 3 family
                     protein"
                     /protein_id="HEX9651151.1"
                     /translation="MGTITRRESLKYITLASISAGMLIRCTPEHEAGVASPVVHNARY
                     KLTEEDLKLLNQQFFTQEEMETVRQLSNLIIPADDHSGNAEDAGVPKFIDFMMLDKPE
                     MQTNVRGGLRWLDFQCLKQFEKEFRSCSVDQQKQLLDQIAYPDNAKPEMSQGVSFFNR
                     FRDLVASGFWSSKMGMEDIGYMGNVPTIWNGPPQEWLDKLGVQA"
     gene            6440..6685
                     /locus_tag="VGA21_11365"
     CDS             6440..6685
                     /locus_tag="VGA21_11365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651152.1"
                     /translation="MLKESINLRDETKQDARDRAHKLFLHGIMSYVNGKIDVFELCDR
                     MIRVDSIIRHVPIDQIRKERKDSLKNKILKKFGASDN"
     gene            complement(6716..9151)
                     /locus_tag="VGA21_11370"
     CDS             complement(6716..9151)
                     /locus_tag="VGA21_11370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014019522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HEX9651153.1"
                     /translation="MRIATSFLILLLLFQLPLLAQRKKNTDKEPVQQETPKPKNGMKS
                     YSEIVTRDAKTDIGLFTVHNVNGNYLFEISDSLLNREILVVSRISGTIEGFNFGGAGM
                     KARGQQVLRWQKKDDLLLLRSVSHNSVASNETPIYESVRNNNFEPIIMSFEIKALSKD
                     SSAYIIEVNELFTTDVPMIGPLNADRRKDFGIRSLDKKRSMVMWMKSFPLNTEIRHIL
                     TFEATQLPSNENTNSLSIEMNQSMVLLPRKPMMARNHDRRVGYFSVRQIDYGLDEQKA
                     AERRFVTRWRLEPKDMEAFKRGELVEPKKQIVYYIDPATPSKWRNYIKQGVNDWNIAF
                     EKAGFKNAITAKDPPSNGEDPDWSPEDVRYSVIRYTANPIQNAQGPHVHDPRSGEILE
                     SDIIWYHNVMNLLRNWYFVQTAAVNPLAQKVKFEDEVMGRLIRFVSAHEVGHTLGLQH
                     NMGASYSYPVDSLRSPAFTATMGTAPSIMDYARFNYVAQPGDNAALLPQIGVYDKWAI
                     EWAYKPIPEANSSYEENETLNSWIMSHENDQMYWFGQSSRSDPRAQTEDLGNNSMQAS
                     SYGIANLKRIVPNLIRWTSGNGKNYDDLDELYNQVVNQWNRYNGHVRNNVGGLYETHK
                     TYDQNGQVYSVPAESLQKEAMAWLHKETFATPVWILNEEVMGLVSGSGMMERVRSLQV
                     ATLNQLLSVDRMLRVSEAGARNNNQTYTLLEMMQDLREGLWSELATAGSIDAYRRNLQ
                     RAYLDRMGWLLENQESTSEDLTQSDIRPIARGELKVLRSRISSAISRIRDTETRYHLE
                     DAIVRIDHILDPK"
     gene            9312..9998
                     /locus_tag="VGA21_11375"
     CDS             9312..9998
                     /locus_tag="VGA21_11375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651154.1"
                     /translation="MHHPYTQHGNIWNSLSAEKQVIYLVCYSSIFLDGIPVRAITEIC
                     GSEPEEVESAITKLINERKITKNGEIISCVNLNISNNKFDNDEKIITRIIRENGFWIH
                     LMKKLPIIRFIGISGSLAAHNPVRQKDRNVDMDLFIITSRYSLWILAFFVKLYTGLKI
                     NKLFKHPLCINYIMDGTFLKIHNPNFYVASEIVNLKPLYGKKMYNQFIEANKWVYNYY
                     PNFSPKANFN"
     gene            10001..10360
                     /locus_tag="VGA21_11380"
     CDS             10001..10360
                     /locus_tag="VGA21_11380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651155.1"
                     /translation="MKFINIFFFVCYQLLRCVKKFSFSPILNISFHFRPLEGKSLYRI
                     NPDLNGYNTFVQEKYRFLVKNNFSEYYDEELINMMFPEAGNSNEPILRNGKFINKNEL
                     PDAKAAQKLEKLFKKYE"
     gene            10353..11156
                     /locus_tag="VGA21_11385"
     CDS             10353..11156
                     /locus_tag="VGA21_11385"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX9651156.1"
                     /translation="MSDYYRQVAKYYDIDAQSFESRYWENEILQKIRQEFREQLYWCD
                     FENALEIGFGTGLDIVHFAKAYPERKIFGIDVSREMFHHCNQKLQKENITNAFIAIGT
                     PEEIPEIFKNQKFELIYVFFGALNTTRDLQQVSIQFHKLLSNNGRLVLTFVNKWYLAE
                     IVIQSLKLNFRKAFSRFKHTWGGYSNEKFLESKCYSPVEIRKIFGKEFELVKKHGYSI
                     LYPAWYRNRWVSKMGRRSANFLWNADRMVNRTFCWKFGEYFLYSLRKRS"
     gene            complement(11132..12295)
                     /locus_tag="VGA21_11390"
     CDS             complement(11132..12295)
                     /locus_tag="VGA21_11390"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX9651157.1"
                     /translation="MKLLILSPQFPSPEFPMDGKSKSQLAIELTKLGVQVEIIVPVPF
                     SLKFKTTLFHIDSEAYDNVITHHPVYIKLPFVKRFYFLLNPLQRWLFYMAVRKRINQY
                     LRQGGKIIHAHSMLFTGYVANKFKAANTCKSIVTFHDNEIQSLSGNIRAYRTFLETEI
                     KKSDLIVFVSKNLRNNYFAVAEAKINSEIIPFPIDHYKFEKSLSKPFTIVGCGKLIPS
                     KGFETLIAAAKLLYLAGNEVEVRIIGEGPEKRNLQNLVRQSGLEKFVCFKGFLRNSEV
                     IAEMSAAHLFVLPSYRESLGLVYLEAMSVSTPVIGVKGQGIEDFIINGENGYLMDAED
                     TGQLYSLIQKLMNDGNLRVRIGKNGFETYRNSLANVRNNAAYHFKLYERFLNE"
     gene            complement(12292..>12734)
                     /locus_tag="VGA21_11395"
     CDS             complement(12292..>12734)
                     /locus_tag="VGA21_11395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651158.1"
                     /translation="PWIVGQGSWFKHFFLDQIKYESKVPLIGEGNNWMSVISLQDCAG
                     LIYHCLNAEVNNIFFNICMPGIYIKMKDFVAIISESVTIPVQPYSTQQAKKLFGSAAT
                     AAFQTSLRLNTNYKDIYLGYRWQHFDIESIIKSNLRIPVNHSEV"
BASE COUNT         3737 a         2548 c         2588 g         3861 t
ORIGIN      
        1 attatatttc cggtttcatt tatcacagca acattacttc caagagcgac caaatccggt
       61 tttatccttc cgtctgaagt cgggcctact ccgctgaatg aagaaatatt tccattaccg
      121 gttaccgaac ctactgtaag aacggtctca acatctccag gcgccagtac atttctccaa
      181 ttagtatttc cctcattgcc tgatccggtt actacaagaa tacctttgct ggccgcgatg
      241 gctgctgccc tgcttattac cgtagtatta ccatccaaat cttcatacgt ataattcatg
      301 cttgcatcgg tgaaagtatt gtatcctatc gatgcattaa tgatatctac tccggcactg
      361 tcagcaaact ccgctgccag aagccaattg tattcttcaa tccggaattc ggaatttata
      421 tcttctgtaa cgaaaagcat atagtccgct tctggtgccg atccggtaaa tcctgaatcg
      481 aaagcgccca ttgttgagaa tacctccgta ccatgatcgt cgtgtaaata cacattgatg
      541 tcattcacag tataatcaaa ggtgtacaga atttttccat tggtataaag gtgggtaaac
      601 tggctgatat tgttgaggtt cgtaaatccc gcatcggtca ccgcaatcag aattccattc
      661 cccttgaatc catcatcatg catttcatca gcgcccatgt agtttatctg gaaatcagat
      721 tcacctgctg ttgttctgtt tttagtattt tttgattcat aatcaacgga gcggccattt
      781 aattttgttc cgggagcaac atattcgact tcagaaacaa aagcaaggtc ttcaataata
      841 gtgattaaag acacatccat ttccacaata gcgacattga accaccttga ggtataaaag
      901 acattgacgc cttcattttc gagttcattt acatagagtt tggttacggg gaaatcttgt
      961 tcggttactg atacattgtt tttgattctc cgggtcagag agcgttctga caaaaatgct
     1021 gatggattgt taatagtaaa ttctgaattg tttttatcag taaaatgaat aacatatctg
     1081 tttatttgcg ccgatgttgt tgtcgaaaca aataagataa aaagaaccag gaaattttta
     1141 attgccccca tttatttggt caaatatagt cttgcaacag gataacaagt tccctgccaa
     1201 atcaatttgg agttattctt tcgattaagg tttttttctc catgaaccca aactctattt
     1261 ccccggcaca taatgtgcac agaatgttat ccggacaaca acaatcggca ttttgattgt
     1321 tcctgcagct ggcatattcc aattggtttg tttccgaata aataaggccg atattttgcg
     1381 cgtacacggc aaattcaaca aggtcttccg ttatctgcag gggatcctga gtattctgcc
     1441 tgattattct tgctgttttg ggataggtga tgccaccggc ggtgaattcc ttatcacggt
     1501 ccgctacttc aaagtaatcg caatactcac ccccatccgg acaatcgttc aaactgtatc
     1561 ttccttccca tctaaatcca ttgagcaacg gaaattgcag ttcaaccgtt tcaatattat
     1621 ctataacgga gattgcggac ccaaattcat gtctgatagt aaaatacttc tttggaatcc
     1681 agtctccatt cagatccgga attgattcaa gaatagtaaa caagcttgag ccatccgcaa
     1741 atacttgctt ttcgactatc tcttcccgca gaagaaattg ttcaacttcc ggttctatca
     1801 accggcccag atagcttacc cggtttagtt cgtaggttga aataactcca acttccaatg
     1861 gaaggaaatc ccaatccagg gatccgcggg agttcacaaa agttggttcc tgacaagaga
     1921 tgaaaatgat tagtaagtaa ctaatccata ataatttttt gagcatgttg cttaaagctt
     1981 gattgaatcc atccgccacc aattacgtca tcgtcttcat aaaaaacggc agcttgccca
     2041 ggtgctattg catgaactcc ctttccaaag taaactttaa tagtgtctcc ggcttgttca
     2101 ataacggcag gagtcccatg atcattgtat ctgactttag taattgtatc cagccttttg
     2161 ccataaaggt tagggtactt atttaaggtg agctttttta cgtacatccc gtcacgggaa
     2221 agttcgtcaa aagtgcctaa aactactcta tttttttctt tttgaatttc agttacatac
     2281 acgggatatc ccaaagcaat ttttaatccc ttgcgttgtc caatggtata gaagggataa
     2341 ccttcatggg ttcctaccac tgttccatct tccaacacaa attcgcctcc ttttacttca
     2401 gcttctaaac cctctactct tcgcttcaga aatcctcggt aatcattgtc aggaacaaag
     2461 caaatttcat aagactcagg cttctttgct aattcaccaa aacctctttc atttgccata
     2521 tctctgattt gaggtttggt aattttaccc aacgggagaa tagtcctgga caggctttcc
     2581 tgggaaacgc cccacaacgc gtagctctgg tctttattca gatcgattcc cctgcttatg
     2641 atatacctgc cattctcttc ccggacatta gcataatggc cagtggctat aaattcacaa
     2701 ttcagtttat ctgcccgccg caagagagca tcccacttaa tatgcgtatt gcataataca
     2761 caaggattag gagtcctgcc tgctatatat tcagaggtaa aatgatcaat tacaaaatcg
     2821 ccaaattcgt ttctgatatc cagaatataa tgcggaaatc ccagggaaac agcaatattt
     2881 cgggcatcat taatcgaatc aagactacag catccggttt cttttttagt gccgcctgag
     2941 cctgcataat cccaggtttt cattgtcatt ccaattactt cgtatccttg ttcatggaga
     3001 agaatggccg cgagagagct gtcgattcca ccgctcattg ccaccaggac acgtcctttt
     3061 ttactcattt ttttacctcc tttctaacgg gttcaaagcc cgccgcggaa ttaaaaacca
     3121 attaaactct attaatagtg aatgaattgc aaatatatcc caatgttttg taagattgaa
     3181 tcataaaaaa gaaaacaaac ccgacatggg actgaaaaca ttggtaaagc ttagccaggt
     3241 aaccaatctg agtgacgcca ggtatgcagc gggaatggga gttcagatgg tgggattcaa
     3301 tattaatcct gagtctgact tttacatcga tcctgataag tttaagggga tcatcgcctg
     3361 gatttccggt gttacattaa ttggagaatt tgaggggtca gtattttctt ataatgccgg
     3421 ctacaatttt gattacattc aaatttcaaa ccctgatttt gttaaagcaa tcaccggatt
     3481 aaaaaagaag cttatcttcc aaattgattt ggacatatat gatgaggttg cagcggaaaa
     3541 aatcatgact gagttgaaag acaaggtcga atactttttg gttacaaaat ctgatgagaa
     3601 actaaatgga aaagaaataa aatcctgggc aagattattc cctataatct tagggttcgg
     3661 gctacaagag gaaaccatag agcaggtatt acaggagtat cctatcaaag ggatttcctt
     3721 gattgggggt gaagaaaagg tggttggatt taaggactat gatcaattgg ctggtattct
     3781 ggaaagactt caaaccgaaa agggttaatt taggtttggt acaaaggata acttatgttg
     3841 aatttgtctg gaatttatga gcttgctatc ttgagagatt aaccttctat tgatgaaaga
     3901 ttttcaaatc aaacaatctg ccaaggaata tgatgttgtt attgttgggt caggtgctgg
     3961 aggcggaatg gctgcaaaag ttttaaccga agctggtgca aaggtagcgg tacttgaagc
     4021 cgggccaaat ttcaattctg aaactgacgg taacatgttt acctggagtt atgacagtcc
     4081 aagaagaggc gccggttcca gggagaaacc tttcggagag tttgatgcct gctacggcgg
     4141 ttggtatctt gaaggtgagc cttacacgac tgctcctgga acccagttta actggtggcg
     4201 ggcaaggatg cttggcggtc ggactaatca ttggggacgg atttcactgc ggttcggtcc
     4261 tctggatttc aaaaggaaaa gtcttgatgg cctgggagat gactggccaa tttcctacga
     4321 tgatattaag ccctactatg atcgggttga caaattcgtg ggaatttttg gaaccaaaga
     4381 aggaatttat aatgagccgg atggaatttt tatgcctccg ccaaagcccc ggggttatga
     4441 attgcttgtt aaaaaagctt gtgatgaaat tggggtacca ataatcccgt cacggctgtc
     4501 cataattact caacccctga atggcagagc accgtgtcat tattgtggtc aatgcggaag
     4561 aagttgtgcc acgcattcca atttttcctc tccttccgta ttgttcccgc ccgcccacgc
     4621 aaccgggaat ctggatctta taactggtgc catggccagg gaggtggttg ccaacagtga
     4681 aggaaaatgt gtgggtttat cttatataaa tactttgact ggagaagagt attttattaa
     4741 gggtaaggta gttatattgg ctgccagtgc ttgcgaatct gcccgtttgc tgctgaattc
     4801 caagtcaccc gcccacccga atggattggc aaattccagc gatgttgtag gtagatattt
     4861 aactgacagc acaggcgcaa gtgccggggc tttaattcct gctcttgttg atggcctccc
     4921 gcataatgag gacggtgtcg gcggcatgca tttgtatgtg ccatggtggt tgaatgacaa
     4981 gaaattagaa tttcccagag gatatcatat tgaaatctgg ggcggccgtg gtatgccggg
     5041 ctatggtttt ggtggtggaa ttcaaaatca gaataattat ctgggcatca tagatgaaag
     5101 tccccgggag aaaggtggcg gtggatacgg tttgactttg aaaaatgatt acaggaaatt
     5161 gtatggagct gttgtgggat tctcaggccg cggggaagca attgcaatgc gggacaaccg
     5221 gtgcgaaatt gacccgaacg ttgtggacaa atatggtatt ccggtgcttc gattcaatta
     5281 cacctggact gaacatgaag tgaatcaagc caggcatatg caggatacgt tcgaagacat
     5341 tattgacgcc atgggtggta tttccctttg gggtaaacct ggtgctgaca gacagtatgg
     5401 tctcacaaaa ccgggtgaaa taattcatga attaggtgtt acccggatgg gaaatgatcc
     5461 gaagaattcg gttctaaaca agtattgtca ggcccacgac gtaaagaatt tgtttgttgc
     5521 agatggtgga ccttttgtat ctcaggcaga taagaatccc acctggacaa tacttgccct
     5581 gtcatggcga acctctgatt atataattca ggaaatgaaa gcgaataatt tatagctatg
     5641 ggaacaatta ccagaagaga atcattaaag tatattacac tggcttctat ctcagccggc
     5701 atgcttatca gatgtactcc cgaacacgaa gccggggttg cttctcctgt ggtacataat
     5761 gccaggtata agcttactga ggaagatctg aaactactaa atcaacaatt cttcacgcag
     5821 gaagaaatgg aaacggtcag gcaattgtct aatctaataa ttccggcaga cgaccactct
     5881 ggcaatgcgg aagatgcagg cgttcccaaa ttcatagact tcatgatgct tgacaagcca
     5941 gaaatgcaaa ctaacgtaag aggcggcctt cgatggcttg attttcaatg cctaaaacaa
     6001 tttgaaaagg agtttagaag ttgttcggtg gatcaacaga agcaactttt agatcaaata
     6061 gcctatcctg ataacgcaaa accagaaatg agccagggag tcagtttctt taatcggttt
     6121 cgggatctgg ttgcttccgg attttggagt agcaaaatgg gtatggaaga tattggatat
     6181 atgggcaatg tccctaccat atggaatggt ccaccacagg agtggcttga taagttggga
     6241 gtacaagcct gaaagcaaaa gtcttattcc cactttaagc gtcctgcctc agaaatttac
     6301 catatctttg taaaaccttt ctgatgggct ctcgtatacc ctgatttatt ccaattaatt
     6361 gagtaaaaaa taataaagcg gtggtggagg ttaccaaatg ccctaagagg gatattaaga
     6421 tgttcaatta ctttaaaaaa tgttaaaaga gagtatcaat ttaagagatg aaacaaagca
     6481 ggatgccaga gatcgggccc ataagctctt tcttcatggc atcatgagct acgtaaatgg
     6541 aaagattgat gtctttgaac tgtgcgaccg tatgatacgt gttgacagta tcatacgtca
     6601 tgttcccata gaccagatca gaaaagaacg taaagacagt cttaagaata aaattctgaa
     6661 gaagtttggt gcaagcgata actgactggc ctgaataaag tccgtgttcc caaatttatt
     6721 ttggatcaag aatatgatca attctaacaa ttgcatcttc aagatgatac cgggtctccg
     6781 tatcccttat tcgggaaatt gctgaagaaa ttctcgatct aagcactttt aattcgcccc
     6841 gggcaattgg tctgatatcc gattgagtta aatcctctga tgtactttcc tgattttcaa
     6901 ggagccaacc cattctgtca aggtaagcac gctgcaagtt tcggcggtaa gcatctattg
     6961 aaccggctgt ggcaagctcc gaccataatc cttctctcaa atcctgcatc atttccagca
     7021 aagtatacgt ttggttatta tttcgggcac ctgcctctga aacccgtaac atcctgtcaa
     7081 cgcttaataa ctgattgagg gtagcaacct ggagcgaccg cacccgctcc atcattcctg
     7141 atccggaaac cagtcccatt acttcttcat taagaatcca caccggagtt gcaaaagtct
     7201 ctttgtgcag ccatgccata gcttcctttt gcaggctttc tgccggaaca ctgtaaacct
     7261 ggccattctg atcataagtt ttgtgcgttt catacaaacc tcctacatta tttctgacat
     7321 gtccattata cctgttccat tggttgacaa cctggttata caattcatca aggtcatcgt
     7381 agttctttcc atttcccgaa gtccatctga tcaggtttgg aacgattcgt ttgaggttcg
     7441 caattccata tgagcttgcc tgcatggaat tattccccag atcttcagtt tgtgcacgag
     7501 gatctgatct gctggattga ccaaaccaat acatttgatc attttcatgc gacattatcc
     7561 aggaatttaa agtctcattt tcctcatagg aagaattcgc ttcaggaatt ggtttatatg
     7621 cccattcaat tgcccattta tcatagactc cgatttgagg taataaggcg gcgttgtctc
     7681 ctggttgagc tacataattg aagcgcgcat aatccattat tgatggcgct gttcccatag
     7741 tggccgtgaa tgcaggcgag cgaagggaat ctacaggata ggaatagctg gcacccatgt
     7801 tgtgctgcaa cccaagagta tgtcctactt cgtgggcaga aacgaatcga atcaaccggc
     7861 ccattacttc atcttcaaat tttacttttt gtgctaaagg atttaccgca gcggtttgaa
     7921 caaagtacca gtttcgaagc aggttcatca cgttatgata ccagatgatg tcgctttcga
     7981 gaatttctcc ggaacgaggg tcatgcacat gcggaccctg ggcattttga atcgggttgg
     8041 cagtatatct gatcacagaa tagcgcacat cctcaggact ccagtcagga tcttccccgt
     8101 ttgaaggcgg atcttttgcg gtaatggcat ttttaaaacc ggctttctca aaggcaatat
     8161 tccagtcgtt taccccttgc ttgatataat ttctccattt ggatggtgtt gccggatcta
     8221 tgtaataaac aatttgcttt ttgggctcca ccagttcccc ccttttgaaa gcctccatat
     8281 ctttgggttc cagcctccat cgtgtcacaa atcgccgttc ggcagccttt tgttcatcca
     8341 ggccataatc aatttgtctg actgaaaaat atcctactct tctgtcatgg ttacgcgcca
     8401 tcattggctt tcttggtaaa agtaccatgc tctgattcat ttcaattgaa agagaattgg
     8461 tattttcatt cgatggaagt tgggttgctt caaacgtaag gatatgtctg atttccgtat
     8521 ttagaggaaa gcttttcatc cacattacca tgcttctttt tttatccagg cttctaattc
     8581 caaagtcctt tctacgatca gcattcaaag ggccaatcat tggaacatca gtggtgaata
     8641 attcatttac ttcaataata taagcggatg aatctttgct taaggcttta atctcaaatg
     8701 acattatgat tggttcgaag ttgttatttc gaaccgattc gtaaatagga gtttcgttgc
     8761 tggccacact attgtgtgaa actgatcgga gaagcaatag atcatctttt ttttgccacc
     8821 ttaaaacctg ttgacctctt gccttcattc ccgctccgcc aaaattaaat ccttctattg
     8881 ttcctgagat tcttgataca actaagattt ctctgtttaa aagactatca gaaatttcaa
     8941 aaagataatt gccatttaca ttatgcaccg tgaataggcc aatatctgtt ttagcatccc
     9001 ttgtaacaat ttcagaatag gacttcattc cattctttgg ctttggagtt tcctgttgaa
     9061 ccggttcttt atcagtattt tttttgcgtt gtgccagaag tggcaactga aataacaaca
     9121 ataaaattaa aaatgaagtg gctattctca tggcatcagg gtttagattt taaataatta
     9181 attaaagcaa taaaaataaa tgtggaaagt taaagaattg cttttttgta aacaacttaa
     9241 tgattgctcg ggggaatggc attaattttg gtgttataac atataaattt atcaactaca
     9301 tttggtttaa tatgcatcac ccctataccc aacatggcaa tatttggaat tcattaagtg
     9361 ccgagaaaca agtgatatat cttgtatgct actcctcaat atttttagat ggaatcccgg
     9421 tccgggcaat aacagagatt tgtggctcgg aacccgaaga agttgaaagt gccattacaa
     9481 aattgattaa tgagagaaag attactaaaa atggtgaaat aataagctgc gttaatctca
     9541 atatttccaa caataaattt gataatgatg agaaaattat cacaagaatt attcgggaaa
     9601 atggcttctg gattcacctc atgaagaaac tacccattat tagatttatt ggcatttcag
     9661 gatccctggc agctcacaat cctgtcaggc agaaagatag aaacgttgat atggatttat
     9721 tcattattac ctcccgatat tcgttatgga ttttagcttt ctttgtgaaa ttatataccg
     9781 gacttaaaat aaataagctt tttaaacatc cactgtgcat taattatata atggatggca
     9841 cttttttgaa aatacacaat cctaacttct acgttgcctc agaaattgtt aacctgaagc
     9901 ccttatacgg caagaaaatg tataatcagt ttattgaggc caataaatgg gtatataact
     9961 attatcccaa tttctcccct aaggccaact ttaactagtg atgaagttta taaatatttt
    10021 ctttttcgtt tgctatcaat tattgagatg cgtaaagaag ttttcattca gtcccattct
    10081 taacattagc tttcatttca gaccccttga aggcaaaagc ctatatcgca ttaatcctga
    10141 tctcaatggg tataatactt tcgtgcagga gaaatataga ttcctggtta aaaataactt
    10201 ttccgaatat tacgacgaag agttgatcaa tatgatgttc ccggaagcag gtaactcaaa
    10261 tgaacctatt ttgaggaacg ggaaatttat aaataagaac gaacttcctg acgccaaggc
    10321 agcacaaaag ctggaaaaac tcttcaaaaa gtatgagtga ttattaccgg caagttgcaa
    10381 aatactatga tattgacgcg caatcttttg aaagcagata ttgggaaaat gaaattttgc
    10441 aaaagattcg tcaggaattc agagagcagc tttattggtg tgattttgag aatgccctgg
    10501 aaattggatt tgggaccgga ttagatattg ttcattttgc gaaagcctat cctgaacgga
    10561 aaatatttgg gattgatgtg tcccgggaaa tgtttcacca ttgtaatcag aaattacaaa
    10621 aagagaatat taccaatgca tttattgcca taggaacacc ggaggaaatt cccgaaattt
    10681 tcaagaacca gaaatttgaa cttatctatg ttttttttgg tgcacttaat acaacaagag
    10741 atttacagca ggtttccatt caatttcaca aattgttaag taataatggc aggctggtat
    10801 taacgttcgt caacaaatgg tacctggcag aaatagttat ccaaagcctg aaactcaatt
    10861 tcagaaaagc gttttcaaga tttaagcata cctggggtgg atattcaaac gaaaaatttc
    10921 tggaaagcaa atgttacagc ccagtggaaa taagaaaaat ttttggaaaa gaatttgaac
    10981 ttgtgaaaaa gcatgggtat tcaatattgt atcctgcatg gtatagaaac agatgggtgt
    11041 ctaaaatggg gcggagatca gctaattttc tttggaatgc agaccgaatg gtaaatagaa
    11101 cgttttgctg gaagttcggc gaatattttc tttattcatt aaggaaacgc tcgtaaagtt
    11161 taaagtgata tgcagcatta ttcctgacat tagccaggga atttctatat gtctcaaacc
    11221 cattttttcc tatccggacc cgcaaattgc cgtcattcat aagtttctgt atcaatgagt
    11281 ataactgccc cgtatcttcc gcatccatta ggtatccatt ttcaccatta ataataaaat
    11341 cttcaatgcc ctgaccttta acaccaataa ccggtgtaga gacagacata gcctcaaggt
    11401 ataccaggcc aagagattca cggtaactcg gtaaaacaaa caagtgagca gctgacattt
    11461 ccgcgattac ttcagagttt ctcaaaaatc ctttgaagca tacaaatttc tctaacccgg
    11521 attgccgaac aaggttttga agatttcgct tctcaggtcc ctcaccgata attctgactt
    11581 ctacttcatt cccagccagg taaagtagct ttgccgcggc gatcagtgtt tcaaaacctt
    11641 tactgggtat tagctttccg catccaacta tggtaaacgg cttacttaag cttttttcaa
    11701 atttgtagtg atcgatggga aaaggaatta tttcactatt aatctttgcc tcggccaccg
    11761 caaagtaatt gttccgcagg tttttagata cgaatactat taaatctgac ttttttattt
    11821 ccgtttcaag aaatgtccgg tatgcccgaa tgttacccga taagctttga atttcattat
    11881 catgaaaggt cacgatggat ttacaggtat tcgctgcttt aaatttattt gccacatacc
    11941 ctgtgaataa catggagtgg gcatgaataa tcttcccacc ctgacggaga tattgattta
    12001 tccgtttcct gacagccatg taaaatagcc accgttgcag agggttaagc agaaagtaaa
    12061 atcgcttaac aaatggcaac ttaatataaa caggatgatg agttatgaca ttatcgtacg
    12121 cctcactgtc aatgtggaaa agagttgtct tgaattttag tgaaaatggt acagggacaa
    12181 tgatttctac ctgcacaccg agtttagtaa gttctatagc caattggctt ttagatttgc
    12241 catccattgg aaattccgga ctgggaaatt gtggtgacag aataagtaat ttcaaacctc
    12301 tgaatgattt accggtattc ttaaattact tttaataatg ctttctatat cgaaatgctg
    12361 ccagcggtaa cctaagtaga tatctttgta attagtattt aacctgagcg aggtttgaaa
    12421 ggctgcagtt gccgcggatc cgaataattt ctttgcctgt tgggttgaat aaggttgaac
    12481 aggaatagtt actgattcag aaataattgc aacaaaatct ttcatcttaa tatatatgcc
    12541 aggcatacag atgttgaaga aaatattatt tacttctgca ttgaggcaat ggtaaattaa
    12601 gcctgcgcag tcttgcaggg aaataacgga catccaatta tttccttcac cgataagcgg
    12661 tactttggat tcatacttta tttggtcaag aaagaaatgt ttaaaccagg aaccctggcc
    12721 tacaatccag ggag
//
LOCUS       DASWHX010000172         3358 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_562725, whole genome shotgun sequence.
ACCESSION   DASWHX010000172 DASWHX010000000
VERSION     DASWHX010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 3358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3358
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_562725"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            228..680
                     /locus_tag="VGA21_11400"
     CDS             228..680
                     /locus_tag="VGA21_11400"
                     /inference="COORDINATES: protein motif:HMM:NF040422.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6438 domain-containing protein"
                     /protein_id="HEX9651159.1"
                     /translation="MKFISKYLATLVVAITLWGCARPPSQSADTVISMQKTACYGACP
                     VYSVEIFDDMKITYLGGENVPVVGEQTGEISRQQLDELIEAFTDSNFFDYESDYMGHV
                     TDLPTTYIQFTYEGKSKKVRDYYGAPQSLKDLENKVEELVRSIFWKED"
     gene            complement(677..916)
                     /locus_tag="VGA21_11405"
     CDS             complement(677..916)
                     /locus_tag="VGA21_11405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651160.1"
                     /translation="MDLNNQPLFEGDMVMSLRYEMGKCIVRQIETGIEYESVKTGEIV
                     SWVKMIDVATDNQKVRKILPGEDENGNSSEEKSSA"
     gene            complement(1015..1317)
                     /locus_tag="VGA21_11410"
     CDS             complement(1015..1317)
                     /locus_tag="VGA21_11410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651161.1"
                     /translation="MNLIKEYPRFVTFIILVITFIYCGYFTQNISNVLNPQPIQENLH
                     AYQSGTVQQTGTVQQTGTVQFEIRDSSVSEILNEAAWFISKRIEQGIRQTSHFIGN"
     gene            complement(1438..2652)
                     /locus_tag="VGA21_11415"
     CDS             complement(1438..2652)
                     /locus_tag="VGA21_11415"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014771369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEX9651162.1"
                     /translation="MRILSFLFVVITFSLCTAPAKEDVLTDDELRELSLELAHKYIVA
                     DGHVDIPYRLREEMEDISIRTEKGDFDYIRSKEGGLDAPFMSIYIPSEYQETGGSKAL
                     ADSLIDMVEGLVAKDPDKFALAFSPDMVEQNFKNELISLPMGMENGAPVESDLGNLKH
                     FFDRGIRYITLTHARDNEICDSSYDTTRTWNGVSPFGREVVAEMNQLGIMVDISHVSD
                     SAFYQVMRITKAPAIASHSSCRAFTPGFERNMNDDMIKKLGENGGVILINFGSTFLDS
                     VSVRKQLENERHIERWLAENELNEKDSLALLYINDYNAAHPMYSDVSVVADHIDHVVS
                     LAGIDHVGLGSDFDGVGDSLPNGLKDVSMYPNLIFELLKRDYSEEDIAKICSGNFFRV
                     WREVENVASSIR"
     gene            2753..2998
                     /locus_tag="VGA21_11420"
     CDS             2753..2998
                     /locus_tag="VGA21_11420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009583707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b5 domain-containing protein"
                     /protein_id="HEX9651163.1"
                     /translation="MKEEDLPQFTINELALRNGTDREEVWVAYKGIIYDVTRSRLWFS
                     GMHYEHWSGQDLTYELKDAPHTELVFDRFKKVGTIKK"
     gene            3000..>3358
                     /locus_tag="VGA21_11425"
     CDS             3000..>3358
                     /locus_tag="VGA21_11425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HEX9651164.1"
                     /translation="MILIADSGGSKTDWRLLDGEKIEQYRSEGMNPYIVDRSNLEKTV
                     EALSASIRNKEITEIFFYGAGCSTPDNNNKISESIQKYFANARISVNHDLLAVARALC
                     SNERGIACILGTGANSC"
BASE COUNT         1008 a          681 c          661 g         1008 t
ORIGIN      
        1 ctttagagat ttgcttttgt gagaaggcgg gtgaaaaggc aagaagtagg acaaaacaag
       61 taaaggtctt tctcataagt tagatttaat tacccccaac aagatattaa taacctgatt
      121 aatctattcg gtaaccataa aattaccaat attgtctttt aagtctgcgg attgacccgc
      181 aatttttgtt cctaattatc tggcattatt ctttctttag gtaatttatg aagttcattt
      241 ctaaatactt agcaacgctt gttgttgcaa ttaccttgtg gggctgtgcc aggccaccct
      301 ctcaatcagc agatacagtc atttctatgc aaaaaactgc ttgctacggc gcttgcccgg
      361 tttattctgt ggaaatcttt gatgacatga aaatcaccta tttggggggt gaaaatgtgc
      421 ctgtggtggg tgaacaaacc ggcgagattt cccgtcagca actggatgaa ttaattgagg
      481 catttacaga ttcgaatttc tttgattatg aatctgatta catggggcat gtgaccgacc
      541 tgccaaccac ctatatccag ttcacttatg aaggaaagtc aaagaaagtt cgtgactatt
      601 atggagcccc gcagtcgctt aaagatctgg aaaataaagt ggaagaactt gtgagaagta
      661 ttttctggaa agaggattaa gcagaactct tttcttcgct gctgtttcca ttttcatctt
      721 ctcccggtaa tatctttcga actttttgat tatccgtggc aacgtcaatc atcttcaccc
      781 aggaaacaat ttctccggtt tttaccgact catactcaat ccctgtttca atttgcctta
      841 caatacactt acccatttcg tatcgaagag acattaccat gtcaccttca aatagcggct
      901 ggttgtttaa gtccatcagc tttggggtct tattatttaa actttttttc tttttgaaaa
      961 acccaaacat ttcaattttt taaaaatttc tccaaaaaga tagggcatat taaattagtt
     1021 gccaataaaa tgtgatgttt ggcgaatacc ttgttcaatc cgtttgctga taaaccatgc
     1081 tgcctcgttc aatatttcgc ttactgaaga gtcccggatt tcaaactgaa ctgttccggt
     1141 ttgctgaact gttccagttt gctgaactgt tccgctttgg taagcatgaa gattttcctg
     1201 aatgggttgg ggattcagta cattgctaat attttgggta aaatatccgc aataaataaa
     1261 agtgataacc agaattatga atgttacaaa tcgggggtat tcttttatca ggttcattat
     1321 aatcaggttc tattacctga ttatactctg cggtagtatg ttagttgaag gaaatcggta
     1381 aatgaacgga attcaggggt gaataaacaa attagcggaa ttattcatct ttgaaatcta
     1441 acggatgctg ctggcaacat tttcaacttc tctccagacc ctgaaaaagt tccctgaaca
     1501 aatttttgct atatcttctt cagaatagtc tcgcttcagt aattcaaaga taaggtttgg
     1561 gtacatggag acatctttta gtccgtttgg cagagagtct cccactccat caaagtccga
     1621 cccaagccca acatgatcaa taccagccag actcaccaca tggtcaatat ggtctgccac
     1681 aacacttaca tccgaataca tgggatgagc ggcattataa tcatttatat acagcagtgc
     1741 cagggaatcc ttttcattaa gttcattctc tgccagccac ctttcaatat gccgttcgtt
     1801 ttccagctgt tttcgcaccg aaacgctatc cagaaaggta gatccaaaat taatcaggat
     1861 aacacctcca ttttcaccta atttcttaat catatcatcg ttcatattcc tttcaaaccc
     1921 cggagtaaaa gcccggcatg aagaatgaga agcaatggct ggagctttgg taattcgcat
     1981 tacctggtaa aacgcgctat ctgaaacatg actgatatcg accattattc caagctggtt
     2041 catttcagcg acgacctccc tgccaaaagg actaactcca ttccatgtcc tcgtagtgtc
     2101 ataggaagaa tcacaaatct cattatcacg agcatgtgtc agggtaatat atcgaatgcc
     2161 tctgtcaaag aaatgcttca aattgccgag atcactttct accggtgctc cattttccat
     2221 tcccatgggg agtgagatta attcattttt gaaattttgt tcaaccatat ccggagaaaa
     2281 ggcgagcgca aatttgtcgg gatctttggc aaccaggcct tccaccatat caattagcga
     2341 gtctgccagg gctttacttc cgccggtttc ctggtattca gaaggtatat agatagacat
     2401 gaaaggagca tccaacccgc cctcttttga ccggatatag tcaaagtcac ctttttctgt
     2461 acggattgaa atatcttcca tttcttcacg aagccgatat ggtatatcta catggccgtc
     2521 agcaacaata tatttatgag ccagctccag acttaattct cttaactcat catcggtaag
     2581 aacatcttct ttagcaggag cggtgcaaag ggaaaaggta attaccacaa aaagaaatga
     2641 aaggattctc atagtgctta ttcaatttag acaatcaatt tactttaact ttggaaagat
     2701 aaccaaaatt acgggcaagg caacaattta ggatttcagg cagcttcact ccatgaaaga
     2761 agaagactta cctcaattca ccataaatga attagcttta agaaacggaa cagatcggga
     2821 agaggtatgg gtggcatata aaggaattat ttatgatgtg acccgatcaa ggctttggtt
     2881 ttcaggaatg cattatgaac actggtccgg acaggattta acttatgagc taaaagacgc
     2941 cccgcatact gaactggttt ttgaccgttt caaaaaagtg ggtactataa aaaagtaaca
     3001 tgatattaat agcggatagt ggtggatcga aaaccgactg gagacttctg gatggggaga
     3061 agatcgaaca gtaccgctcc gaagggatga atccttatat tgtggacagg agtaacctgg
     3121 agaagactgt ggaagccctg agcgcaagta ttagaaataa agaaattaca gaaatatttt
     3181 tctatggtgc cggatgctca acccctgata acaataacaa aatttcagaa tcgattcaga
     3241 aatactttgc caatgcaagg atttcggtaa atcatgatct tcttgctgtt gccagagcac
     3301 tatgtagtaa tgaaagagga attgcctgca tcctgggtac cggggcaaac tcttgttt
//
LOCUS       DASWHX010000173         1827 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_3496552, whole genome shotgun sequence.
ACCESSION   DASWHX010000173 DASWHX010000000
VERSION     DASWHX010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 1827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1827
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3496552"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..408
                     /locus_tag="VGA21_11430"
     CDS             <1..408
                     /locus_tag="VGA21_11430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009581193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEX9651165.1"
                     /translation="ALREKHGFAYSIDANYNPFMDTGLFSILFGTDPKQLERSVHIVF
                     KELEKLKSKSLGKVQLHRAKEQLMGQLAMAEENNTSLMLMMGRSMLDLDKIDSLETVF
                     DHIRKIKSRDLQNIAHEIFEFDSLSMLKFVGNA"
     gene            423..1067
                     /locus_tag="VGA21_11435"
     CDS             423..1067
                     /locus_tag="VGA21_11435"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019940359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEX9651166.1"
                     /translation="MEFLDEKLSRYAEMHTFPESGLLARINRETFSEVLMPRMLSGHL
                     QGRILSTFSRMIKPKRILEIGTYTGYSTICLAEGLLQDGKIITIDINQELEVRVRKYF
                     KESGFDDKIDFRIGNALEIIEELDEKFDIVFIDADKENYTNYYHLVFEKVNHEGFIVA
                     DNVLWSGKVLDENTADPETAAIIEFNDQILKDKRVHPVLMPVRDGLMVIQKLNK"
     gene            1064..>1827
                     /locus_tag="VGA21_11440"
     CDS             1064..>1827
                     /locus_tag="VGA21_11440"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEX9651167.1"
                     /translation="MKRSVIVILLLLPFIVWGQKIEVPTQLEFAGIKLKIKEDARGEI
                     QEDINALTQNEKFFLNRLSKVVEYFPIIEKIFAEEGLPDDFKYLIIQESAFIPDAVSS
                     SNAVGYWQFKKETALEVGMRVDSRIDERMHIIPSTRGAARYLQKNNYFFNNWLNALQA
                     YQMGAGGALEALGGDKDSGASSMIIDKKTYWYVKKYLAHKIAFEAGLKMYKPQGNSLV
                     EFTRGENKSLKEIAKEVNVEENLLASYNKWLHVKKIP"
BASE COUNT          612 a          297 c          408 g          510 t
ORIGIN      
        1 gccctcaggg aaaagcatgg atttgcatac tctattgatg caaactataa tccttttatg
       61 gataccggcc ttttttcaat tttatttgga accgacccca aacagttgga aagatctgta
      121 catattgtat ttaaggaatt ggaaaaactt aaatctaaaa gcctgggtaa ggttcagctg
      181 catcgcgcta aagaacaatt aatggggcaa cttgcgatgg ccgaggaaaa taatacttct
      241 cttatgctaa tgatgggtcg cagcatgctt gatttagata aaattgacag cctggaaaca
      301 gtttttgatc atattcggaa gattaaaagc agggacttgc aaaatattgc ccatgaaatt
      361 ttcgaattcg acagcttaag catgttaaaa ttcgttggta atgcttaatt ttatatccca
      421 taatggagtt cttagacgaa aagttaagcc gttatgcgga aatgcacacc tttcctgaat
      481 caggactgct ggcgagaatt aaccgggaaa cattttcaga agttctaatg ccccgaatgc
      541 tttcgggtca tcttcagggt agaattcttt ctactttctc aagaatgatc aaaccgaagc
      601 gcattcttga gatcggaact tatacagggt attcaacaat ttgtctcgca gaaggccttt
      661 tgcaagatgg aaaaataatt actatcgaca ttaaccagga acttgaagta cgcgtaagga
      721 aatattttaa ggagtcagga tttgatgata aaatagattt cagaatcggc aatgctttag
      781 agattattga ggaattagat gaaaaattcg acattgtttt cattgatgcg gacaaagaga
      841 attacaccaa ctattaccat cttgttttcg agaaggtgaa tcatgagggg tttattgtgg
      901 cagacaacgt actttggagc ggaaaagttt tggatgaaaa tacagctgat ccggaaacag
      961 ccgcaattat tgagtttaat gatcaaatat taaaagataa gcgtgttcac ccggtactaa
     1021 tgccggttag ggatgggctg atggtaattc aaaaactaaa taaatgaaaa ggagtgtaat
     1081 cgtcatcttg cttttattgc catttatagt atgggggcag aaaattgagg ttcccactca
     1141 gttggaattt gcagggataa agttaaaaat aaaggaagat gccagaggag aaattcagga
     1201 agatataaat gctcttactc aaaatgaaaa gttttttctc aatagacttt ccaaggttgt
     1261 tgaatacttt ccaataattg aaaaaatatt tgcagaggaa ggattacccg atgattttaa
     1321 gtatttaata attcaggaaa gcgcgtttat tcccgatgca gtcagctctt cgaacgcggt
     1381 gggatattgg caattcaaaa aagaaactgc actggaagtt ggaatgaggg tagattcacg
     1441 aattgacgaa aggatgcaca ttatacccag cactcgcggc gcagcaagat atctgcaaaa
     1501 aaataattac ttctttaata actggctgaa tgctttacaa gcttatcaga tgggagccgg
     1561 cggcgcattg gaagccctgg gtggcgataa agacagcggt gcaagttcca tgattattga
     1621 taagaaaact tactggtacg tcaagaaata ccttgctcac aaaattgctt ttgaagccgg
     1681 gttgaaaatg tataagccgc aaggtaattc actggtagaa tttaccaggg gggagaacaa
     1741 gtccctgaag gaaatagcaa aggaagttaa tgttgaagaa aatttgctgg ctagttacaa
     1801 caaatggctg cacgtgaaga aaattcc
//
LOCUS       DASWHX010000174         3429 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_566681, whole genome shotgun sequence.
ACCESSION   DASWHX010000174 DASWHX010000000
VERSION     DASWHX010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 3429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3429
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_566681"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(45..2483)
                     /locus_tag="VGA21_11445"
     CDS             complement(45..2483)
                     /locus_tag="VGA21_11445"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9651168.1"
                     /translation="MIIHYLTTSFRFLRRNAVFSLINIAGLSIGLASCIVIFLYLLSE
                     LSYDRFWTHHERIFRINSSWNSEKGTARYATSPPTLVPTLLNELPEIEAATRIFKWND
                     FTLRPDSDSSNVFRETNVFMADENYFRVFQDRLIVGNPLTALKNPVSIVLTESAAIRY
                     FGREFYESNVIVGRNILGGRDGGTLWQITGIMKDIPINSHQNFEILISSKTFGDEFDN
                     SQVWTWNVMYSYVLLNEQAKSESLNEKLNSIIDRYVIPYISYSARDLATFGQPMSFGF
                     MPLTDIHLKSNYLKEISLNGSIRLVYVFLSVAVLILLIACVNFMNLSTALSSRRAKEV
                     GIRKSLGSHYRSLLIQFLGESVLVSLISMLMALGISELFMIYLRNTMDLQFLYTPYNS
                     PYFIPALIFISIFAGMLASLYPSIFMSNLKPVAVLKSKAINLSRNSIRNVLVVFQFMI
                     SVGLIICVLVIRDQVEYILSKDLGFEKENVLVIQNDREIDERREEFKSRLLQYPAIVA
                     ASFSTGIPALNEFRIRDYKVEGSQTSIGFRWHEIDDSFIKTLDLDLIDGRNFSGLIAS
                     DSSGLILNEAAVAELGLTQPIGKTIIINEGDVDERKVTVLGVVKNFNYESLRREIMPL
                     GMEFLHTYYFKDYVSIRIQPGMERAAVKIIQELWKEFEPDVPITYSFLDSNFDSMFKT
                     EMTMEKIFKSLTGLAIFISCMGLFGLAAFSTEQKRKEIGIRKVLGASAPGIFILFSSR
                     FIKLSSIGFLLAAPVTFYFTNEWISGFAYHSPISPWNFLIAGGITILLAILTVAYQSI
                     KLSLVNPAEILKED"
     gene            complement(2485..>3429)
                     /locus_tag="VGA21_11450"
     CDS             complement(2485..>3429)
                     /locus_tag="VGA21_11450"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX9651169.1"
                     /translation="GNSFEDLKNKLENLDGVINISAVIRLPGSDNSFSITSLSVPGSE
                     ESLRVNWFRGDHDFINTLEFELIEGRGFDENLSSDSVSIILNEAAVKALSLKDPVGTI
                     LNDLKVVIGVVKDFHFESLRNNIGPAAISLHPEQGHKMAIKIEPGKYQNVIEFINREW
                     NGNLLNNPLNYFFLDDNFGKLMKKDEEMARVVSLFTVLAIFISCLGLFGLSSYLAVLK
                     KKEIGIRKVMGASITNILFLLNWKFSRLVLLAIILAIPITAYLMNWWLKGFAYKIELS
                     ATYFLLASTLTILLAWGTVSYQSIMAAFSNPVNSLREE"
BASE COUNT         1101 a          704 c          590 g         1034 t
ORIGIN      
        1 tgacctttct gcgcttctct ccctaagatt tgaagcaaaa aatcttaatc ttctttgagt
       61 atctcggcgg gattaaccaa tgataattta atggattgat acgccaccgt taagatagcg
      121 agcagaatag taattccacc cgcaattaaa aaattccagg gtgaaattgg tgaatggtag
      181 gcaaatcctg aaatccattc attagtaaaa taaaaggtta caggtgctgc caggagaaaa
      241 ccaatggatg ataatttaat gaaccgactg ctgaataaaa taaatatgcc cggcgcagaa
      301 gcacctaata cctttctgat gccgatttct tttcgttttt gttctgttga aaaggctgct
      361 aatccaaaca atcccatgca tgaaatgaaa atggcaagac ccgtcagtga cttaaaaatc
      421 ttttccatgg tcatttcagt tttgaacatc gaatcgaagt tggaatccag aaagctgtag
      481 gtaattggta catccggttc aaattccttc cacaattcct gaataatttt aaccgcggct
      541 ctttccatgc ccggctgaat tcttattgaa acataatctt tgaaataata cgtatgaagg
      601 aattccatac ccagcggcat aatttcccgt ctcagggatt cataattaaa gttctttaca
      661 accccaagga ccgttacttt tctttcatcc acatctcctt cattaataat tattgttttg
      721 cctatgggtt gcgttaaacc aagttctgca acagcagctt cattaagaat taatcccgaa
      781 gagtcagagg caattaatcc tgagaagttt cgaccatcaa tcaaatccaa atcaagagtt
      841 ttgataaaag aatcatctat ttcatgccat ctaaacccaa tactggtttg agaaccttcc
      901 actttataat ctctgatcct gaattcattt aatgcaggta ttcccgttga gaaggatgcg
      961 gcaacaattg ccgggtattg cagcaaacgt gatttaaact cttccctcct ttcgtctatt
     1021 tcccggtcat tttgtattac cagaacattt tccttttcaa acccaagatc tttgcttaga
     1081 atgtattcga cctggtcccg gataaccaaa acacaaataa ttaatcctac tgaaatcatg
     1141 aattgaaata caacaagtac atttcgaatc gaatttcgac taaggtttat tgctttgctc
     1201 tttaaaactg caaccggttt taggttcgac ataaatattg aaggataaag acttgccagc
     1261 attcccgcaa aaatggaaat gaaaattaat gccggaataa aatatggact attgtatgga
     1321 gtataaagaa actgcaggtc cattgtattc cttaaataaa tcataaataa ttccgaaatt
     1381 cccaacgcca tcagcattga aattaaactc actaacacac tttctccaag gaactgaatt
     1441 aataagctgc gataatggga tccaagggat tttcgaatac caacttcttt agccctgcgt
     1501 gaagataaag ctgtggacag gttcatgaaa tttacacagg caatcaataa aattaagaca
     1561 gcgaccgaca ggaatacata tacaaggcga atgcttccgt ttagagatat ttcctttaag
     1621 tagttggatt taaggtgaat atcagttaat ggcataaagc caaatgacat cggttgacca
     1681 aaagttgcca aatcccgggc actataactt atatatggaa ttacgtaacg gtctattatc
     1741 gaattcagtt tttcatttaa gctttctgat tttgcttgct cattgagaag tacgtaagaa
     1801 tacattacgt tccatgtcca tacctgggag ttatcaaatt catctccaaa agttttagaa
     1861 cttattaata tttcaaaatt ctggtgagag ttaattggta tatctttcat gatccctgta
     1921 atctgccaaa gagttccacc atccctgcca ccaaggatat ttctacccac tattacattg
     1981 ctttcataaa attcccttcc aaaataccgg atagcagcag attctgtcaa aacaattgag
     2041 actggattct tcagagcagt caacggattt ccaacaatca gtctgtcctg aaatacgcgg
     2101 aaataatttt catctgccat aaacacattt gtttccctga atacattcga tgaatcggaa
     2161 tccggcctta atgtaaaatc attccattta aaaattctgg ttgctgcttc tatttccgga
     2221 agctcattta gcaaagttgg tacaagggta ggcggcgaag ttgcatagcg ggcagtacct
     2281 ttttcggaat tccaggaaga gttaattctg aatattcttt catgatgtgt ccagaatctg
     2341 tcatagctga gttcactgag caagtacagg aatataacaa tacagctggc aagccctatg
     2401 gaaagtcctg ctatattaat cagactaaat accgcattcc tccttaggaa cctgaagctg
     2461 gttgtcaggt aatgtatgat catatcattc ttctcttaaa gaattaaccg gatttgaaaa
     2521 tgctgccata attgactgat atgaaactgt tccccaggct aacaaaatgg tgagcgttga
     2581 tgcaagcaga aaataagtgg ctgataattc aattttgtaa gcaaatccct ttaaccacca
     2641 attcatcagg taagcggtta ttggaattgc taaaattatt gcgagtaaaa caagccgtga
     2701 aaatttccaa ttcaacagga aaaggatatt tgtaatagaa gcgcccataa cttttcgaat
     2761 tccgatttcc ttctttttca gtacagctaa ataggatgac aacccgaata agccgaggca
     2821 ggaaataaaa atagccagaa cggtaaatag tgaaactact ctggccattt cttcatcttt
     2881 cttcataagt tttccaaagt tatcatcaag aaaaaagtaa tttagcgggt tgtttagaag
     2941 attgccgttc cattcccggt taatgaattc aataacattt tgatatttcc ccggttcaat
     3001 tttaattgcc attttgtgac cttgttccgg atgcaacgag atggctgccg gaccaatatt
     3061 attacgcaga gattcaaaat gaaaatcttt gaccacacca attacaactt tcaagtcatt
     3121 caaaattgtg ccaaccggat ccttaaggct taatgctttc accgcggcct catttaatat
     3181 tatcgaaaca ctgtccgatg aaaggttttc atcaaatcct cttccttcta tcagctcaaa
     3241 ttcaagggta tttataaaat catgatcacc acgaaaccaa tttacccgca atgattcctc
     3301 agatcccggt acactaaggc tcgttatgct aaaactgtta tcagatccgg gcagccggat
     3361 tactgcagaa atgttgatca ctccgtcaag attctccagt ttattcttaa gatcttcaaa
     3421 actgttacc
//
LOCUS       DASWHX010000175        13454 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_1290424, whole genome shotgun sequence.
ACCESSION   DASWHX010000175 DASWHX010000000
VERSION     DASWHX010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 13454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13454
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1290424"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..389
                     /locus_tag="VGA21_11455"
     CDS             <1..389
                     /locus_tag="VGA21_11455"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEX9651170.1"
                     /translation="VATFNSNAENNGRYYSYVREDISVPTYFRLRQTDIYGVSETYNP
                     VYADCNLNDSEILIYPNPSNGKVTLANLVTGTPIIIQNLKGEILIVTYARSKEETIDL
                     NSFPSGVYLIRIRDGKKEEIYRLAKQ"
     gene            complement(505..960)
                     /locus_tag="VGA21_11460"
     CDS             complement(505..960)
                     /locus_tag="VGA21_11460"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEX9651171.1"
                     /translation="MDTIKSINAWLDSRHPPAGMDFIRILLGIFIVYKGVQFTSNFQQ
                     FTADIESIGWAFIAAHAAQYILFIHFVGGLLIVMGAFTRAMCLLNLPILAGAVVFNSQ
                     KYFTMQNMELPVAAIVLALLIIFFIYGSGRISVDELRRKGKKAETAVNP"
     gene            complement(1178..1333)
                     /locus_tag="VGA21_11465"
     CDS             complement(1178..1333)
                     /locus_tag="VGA21_11465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651172.1"
                     /translation="MELRQEYPELYRNLDEIPITIPTLENPEIDLKTYSEFLESLQHL
                     ILHFKKK"
     gene            complement(1737..2243)
                     /locus_tag="VGA21_11470"
     CDS             complement(1737..2243)
                     /locus_tag="VGA21_11470"
                     /inference="COORDINATES: protein motif:HMM:NF017758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF892 family protein"
                     /protein_id="HEX9651173.1"
                     /translation="MKKIENLNDLFIEQGHELFDIFNQEQKELISIQKQASSRELKRV
                     IDRQLAGVNYQVKRMEEILEKVDAGPGGEKSECCKAIFKQTRDLIKRSLDNEVRDASI
                     ISSLQMLNHYKIAGLGSLAAYAREIGQHDIATFFYETLSEEKAIDRELSNLAEHGINK
                     KALTAMSY"
     gene            complement(2389..2595)
                     /locus_tag="VGA21_11475"
     CDS             complement(2389..2595)
                     /locus_tag="VGA21_11475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651174.1"
                     /translation="MRRLRLRTVLGTLFMIPLLFVSVHSVAQEVDPYEKAKAEVIKTF
                     GSFPDFNSYTSTSILNIQNFSSPG"
     gene            complement(3127..3252)
                     /locus_tag="VGA21_11480"
     CDS             complement(3127..3252)
                     /locus_tag="VGA21_11480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651175.1"
                     /translation="MELKIYHHICKHFLKETIDLFDKTMQKKIEAFDDTEKPVDW"
     gene            complement(3252..3713)
                     /gene="tnpA"
                     /locus_tag="VGA21_11485"
     CDS             complement(3252..3713)
                     /gene="tnpA"
                     /locus_tag="VGA21_11485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018342832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HEX9651176.1"
                     /translation="MMSTYSQIYIQTVFAVKGRESLIKPEWGEQLYKYISGIVRNKGQ
                     KMLSINGMSDHIHFFIGLKPTCCLSDLVREIKKSSNAFIKEKKFTLSNFSWQEGFGAF
                     SYSHSQIDDVCKYIQNQKEHHKKKTFKEEYIEFLHKFEVEHNEQYLFDWIE"
     gene            complement(4123..4194)
                     /locus_tag="VGA21_11490"
     tRNA            complement(4123..4194)
                     /locus_tag="VGA21_11490"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4159..4161),aa:Arg,seq:ccg)
     gene            complement(4214..5143)
                     /gene="mmuM"
                     /locus_tag="VGA21_11495"
     CDS             complement(4214..5143)
                     /gene="mmuM"
                     /locus_tag="VGA21_11495"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF007020.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047150 - betaine-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase"
                     /protein_id="HEX9651177.1"
                     /translation="MGRKTNIIEYPILLDGGLSNQLEELGCNLNNSLWTAEVLRTNPE
                     AIIQAHFNYLEAGAKIITTSSYQATLKGFKDKGLDKVEAEALILKSVELARVAIERYS
                     QKYCRQETTLVAASIGPFGAYLADGSEYRGNYTIHEMELFDFHEAKIRLLDSGSADLL
                     AFETIPSFSEAKIIAAILESTATPSWISFSCRDEEHICDGTPIAECTEMLKAVPGIWA
                     TGVNCTRPGYVKGLIQKIKNQGWEKKIIVYPNSGEKFNAENKSWENSRDILDNNLVEE
                     WLEAGADIIGGCCRVGPQQISAMNKVLISRKRF"
     gene            5567..5761
                     /locus_tag="VGA21_11500"
     CDS             5567..5761
                     /locus_tag="VGA21_11500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014022331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HEX9651178.1"
                     /translation="MKEGKVKFFNYSKGFGFITPSDSGDDIFVHESGLIDRIKENDKV
                     KYEEEPGKKGMNAVRVEVIG"
     gene            5917..7608
                     /locus_tag="VGA21_11505"
     CDS             5917..7608
                     /locus_tag="VGA21_11505"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX9651179.1"
                     /translation="MNKFQKLGLSLPVTEVLNQIGFETPTPVQEKAIPLLLGNDPTDF
                     IGLAQTGTGKTAAFGLPLIDLIDHTSSATQVLIMAPTRELAQQTAKQLTLFSAGKNEL
                     NIVVAYGGAAISNQIKLLKKPAHIVVATPGRLQDLIDRKAVRLNNVRYVVLDEADEML
                     NMGFKEDIDKILSYTPKDKVTWLFSATMPAEIRKIVNKYMDKPIEISINTAELINKDI
                     SHKYVVTTSSNKIPALRRFLDIQPDMRGIMFCRTRRETQHISDELTNLGYGVEALHGD
                     LSQGQRDAVMKRFKMRSMQLLIATDVAARGIDVNDLTHVMHHTLPDQPDIYTHRSGRT
                     ARAGKKGISLAFINPREGKRISELERRMKVKFERIEVPAVEEMRYSRINSWANLIINT
                     HVDDKAEEVLQHLHGQFAHLSKEDILRRLITTQLDHLIFQHNDEDLNEKSDAGARNGK
                     KDAASNRYFVNIGIIDGMTKGDLIHFLSDVSKVDRKHFGEISMHKNYSFFQISSAVDK
                     KISDSFMGVEIEGRQIRVNLDENKRSGRDSSGGKGRMSGKRSWNSKSFKRNIKRR"
     gene            7612..7797
                     /locus_tag="VGA21_11510"
     CDS             7612..7797
                     /locus_tag="VGA21_11510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEX9651180.1"
                     /translation="MGRQNQNAFIKRKKEEEKRKKKKEKQEKKEARKQEPSSSKLEDM
                     LAYVDKDGNITNQPPEG"
     gene            complement(7841..8887)
                     /locus_tag="VGA21_11515"
     CDS             complement(7841..8887)
                     /locus_tag="VGA21_11515"
                     /inference="COORDINATES: protein motif:HMM:NF013215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651181.1"
                     /translation="MGSIKTGITRKRRKKIRESEKVLGWDYFDRKGKRITNLQIIDRC
                     NKLVIPPAWEDVWISHDPKSDLQATGKDAKGRLQYRYHASWTEFRSAEKFESLTMFAS
                     LLPDIRKKIDRDLSLTGMPKDKVVALVIKLMDLYHIRVGNDEYARKNNSYGLTTLKEG
                     HMRIDRSKTAEGELDAILEFRGKSGKDWNIRIWEDDLAILIDESGKVGGRHKSQDLFR
                     YEDSTGKDFDIKSYHINEYLDSLVPSDKKVTAKDFRTWAATWKAASRLSLQIDPDTIT
                     ARKKVATQVIKTVASDLGNTVAVCRSSYIHPVVLGDWHEGMFRPKWNSSGKARKIQGL
                     SKDETVVLRYLDHS"
     gene            9034..11031
                     /locus_tag="VGA21_11520"
     CDS             9034..11031
                     /locus_tag="VGA21_11520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007652538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX9651182.1"
                     /translation="MQLPILQDIVIILGLSVVIILLFQKLRLPAILGFLLTGIIAGPH
                     GLNLISATHEVELLSEIGIIFLLFVIGIEFSLKGLSTVKNTVLIGGLFQVGGTIALTA
                     LFSYMLGMTIPSAIFMGFLISLSSTAIVLKLLQEQGELTSPHGRISVAILIFQDIIVV
                     LMMLLTPLLAGEMENPGTTLAILVVKVVGVIVVVIALARYIVPNLFTLVVKSKSQELF
                     ILTVVVICFATAWLTSSIGLSLALGAFFAGLIISESSYSHQATANILPFREIFISFFF
                     VSVGMLLDLSFFIDHVVNILLLAIGVIFLKIIVIAITVLILNYPPRTIILTALSLFQV
                     GEFAFLLSTSGMKYNLLSQEFYQYFLAISIITIGITPFIMKFSSSIAQFILHAPLPGR
                     VKRRLKVLTHHKAETTAKHEDELKDHLVIIGYGINGENVAKTARQAKIPYVIVELDPV
                     AINKANRNKEPVVFGDASNDVILKHVHIQEARVIVIAISSPESTKRIMRRVREFTKTA
                     FVIVRTRYVREVEEIVGLGADVVIPEEFETSIEIFTRVLKKYLMPNNEIMEFVNQIRS
                     HNYEMLRSIPGFSVVTQANIPDMEIATLPVHQGVNKVVGKSVQESGIRSNFNITVLAI
                     KRGEKYITRVTPETIITQDDILFLFGSPNSIIALNKFLMLN"
     gene            complement(11051..>13454)
                     /locus_tag="VGA21_11525"
     CDS             complement(11051..>13454)
                     /locus_tag="VGA21_11525"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9651183.1"
                     /translation="ISYNHKYSSSTLMLPAMFRNIIKTTFRSLLKQKSYTFINTLGLT
                     VGLASSFLILLWVEDEYRVDGFHEKSANIYQLKRIIYQDDGQNFNTEAIPQPLEIALE
                     ETYPEIDMVTLIGWDIEALFKLEDRSFKETGRYVSPEFLKIFSFPLIIGSKENALNEI
                     NSIVISEDLAAKLYGENWQSNILGSTLPLGNNTFTISGVFENPSPHSSLQFQWLIPAQ
                     RYISENSWVENWNNGGFQMVVLMKDGADINAFGEKVLNEVNNNIQGGDERIVYQKFSD
                     QYLYSNFENGAAQGGRIDYVRALLIVAICIIIIACINFMNLATARSAIKVKEIGVRKV
                     MGAGRNSLALQFLTEALAIAFTASFFAILIVQLILPGFNQLTGKFIQIDFSAPTYWLM
                     ITGIALVTGLLSGMYPAFFLSGINTIHVIRGHIIKVGGGATLRKALVVFQFAISIVLI
                     IGSVSIFRQIMFILDKNLGLDRENIIYVPIEGAILQNFDAYRTELLRIPSISSVASSD
                     QNPLGVGRSTGGANWDGKDPSFQIEISVLSTDHNFLKTMKMELISGSDFSGDFYRDST
                     SFIINEQLARLMNKDNPVGENFSLWGINGKIIGVVRNFHMESLHEAIEPLIIRSNAIG
                     RLAFIRISGNIQQTVQEIEKVTRRLNPGYPFQYTFLDDQFEQDYKDEMMIADLASYFT
                     VIAIFISCLGLFGLSSFIAQQRVKEIGIRKVLGASITNLFMLLSLNFGKLIIIAFILA
                     APVGIYFSNNWLEQFTFRISIGPGIIIAAGLGALIIAVATVSFKAIQTALANPVDSLK
                     DE"
BASE COUNT         4042 a         2637 c         2610 g         4165 t
ORIGIN      
        1 ccgtcgctac atttaattcc aatgcagaga ataatgggag gtattacagt tatgtccgtg
       61 aggatatttc tgtaccgaca tactttcgtt tacgccagac agatatttat ggagtaagtg
      121 aaacgtataa tcctgtttat gcagattgta atttgaatga ttctgagatc ttgatttatc
      181 caaacccctc aaatggaaaa gtaacattag ctaacctggt taccggcact ccaattatta
      241 tccagaattt aaaaggtgaa attcttattg ttacctatgc cagatcgaag gaggaaacaa
      301 ttgatctaaa ttcttttcct tcaggtgttt acttaattcg cattcgggat ggtaaaaagg
      361 aagaaattta cagattggct aagcaataaa cctcaaatca ggatcgcctg ttgcttttac
      421 agtcataaat catggattca atccattgta tttccatttt ggaaaattta ctgaaggaaa
      481 atcaagccgt gggtagttgc ggtactacgg attaacggca gtttccgcct tcttaccctt
      541 tcttctgagt tcatcaacgg aaattcttcc gcttccgtaa atgaagaaga tgatcagcaa
      601 tgcgagtaca attgccgcaa caggcagttc catattttgc atggtaaaat acttctgcga
      661 gttaaacaca actgcacccg cgaggatggg taggttaaga agacacatgg cccgggtaaa
      721 agcacccatt acaataagca agccaccgac aaagtgaata aacaggatgt actgagctgc
      781 gtgtgctgca ataaatgccc aacctatcga ttcaatatct gcggtaaatt gttgaaaatt
      841 gcttgtgaac tgaacgcctt tgtaaacgat aaaaattcct aacagaattc ttatgaagtc
      901 catgcctgcg ggtggatggc gtgaatcaag ccaggcattg atacttttga ttgtgtccat
      961 tgcatctgat ctatgtccaa tgaatgtagg aatttgaaag atatgaagca atgaaaatga
     1021 aaaatttctg gtatggaaat aataaaatgt attgtgatgc cgaaggtcta tacacccctg
     1081 aataaataac tatacattaa aaattccgct aatgcgcaag caaggaccca aagttgcaat
     1141 tgcgatgaga tatataatct tcatttttag tttatgttta tttttttttg aagtgaagga
     1201 taaggtgttg aaggctttcc agaaactcgg agtatgtttt caaatcaatt tcaggatttt
     1261 caagggtggg aatagtaatt ggaatttcat ccagatttct gtaaagttcc gggtattctt
     1321 gcctcagttc cattagaagt ttatttatct cccttaggat ttgatgttga tttttctgag
     1381 cagtcaaatc ttgtaagttt aaagttcaag tcaaatactt aacaaaccat ttcgcttatt
     1441 accttgcaac ttcctccaat tccccctctc cgggaatgcc tggttttcca tataagcgtt
     1501 acatcatcac cggcggaatt gataatttct ccttgttatg gatggtaatt cataaatttt
     1561 tcaaaaggaa tgttcttaat tctactattt aaagatagga aagtgtacgg gcttttgaaa
     1621 gaaaataatt acccttcaaa aatgatcatt atcaggttct gagaatatat attccggccc
     1681 agattattga gtgaggggtt tattaacccg caatagccac tctttattga ttgcattcaa
     1741 taagacattg ccgtcagcgc ttttttattg ataccatgtt ctgccaggtt agacagctct
     1801 ctatcaatag ctttttcctc agataaggtc tcgtagaaaa atgtagcaat gtcgtgttgt
     1861 cctatttctc tggcatatgc agctaatgat cccaacccgg caattttgta gtgattgagc
     1921 atttgcaatg agctaatgat ggatgcatct ctaacttcat tatccagact tctttttata
     1981 aggtctctgg tttgcttgaa aattgccttg caacattcac ttttttcgcc accgggtcct
     2041 gcatccactt tttcgagtat ctcttccatt cgctttacct ggtaattaac acctgccaat
     2101 tgccggtcaa tcactctttt tagctcacga ctgctggcct gcttttgtat agaaatcaac
     2161 tccttttgtt cctgattaaa aatatcaaat agctcgtgtc cttgttcaat aaacaagtca
     2221 ttgaggtttt ctatcttttt cataattaaa aatttgtgtt attgaatcct ttaaaaccaa
     2281 tgtacaggga ttatctctta tgctcaatga tattcgtcag gtagtgagct tgtatccaga
     2341 atgatctcga tgcagggtta tagaagccat tattcgggct caaatatatt aacccggaga
     2401 agagaaattt tggatgttta aaatagaagt ggaggtgtag ctattgaagt ccgggaatga
     2461 accaaaagtc ttgattactt ccgctttggc cttttcatac ggatcaactt cctgcgctac
     2521 tgaatgcaca gaaacaaaca gtaaaggaat catgaataat gttcccaata ccgtcctgag
     2581 tcttaatctt ctcatggttt agaggtttaa ttatagaaat atgtttccca tggatgtaaa
     2641 tattaaaggc aagacctcaa agcagctcct tgagaatttt ccatcaagat ataaaatttc
     2701 cactttagat agaatgtttc cagataatct tcacttatta atcttaaaat cttaatgctt
     2761 acgcaaatta attgggaaaa agtaacacat aatagtctac tcattagatg agggtcggta
     2821 aaaaaatttc agaaagcatt agcagaatgc agggctagtc aaaaaaaatc gcagacattt
     2881 ttccaaaggg ggtaatgaaa gcagataaag aagaggaacg cgaggggaca cttatggaat
     2941 tcatcattcg taattcttaa aatatattta tcaatgacag aaactatttt catttttgag
     3001 aattctggta gatactatat tcttcttcac ttctatttgc atctttatca tttttagatt
     3061 cgaaagattc gaaatattga cataattttt ggggttgatc tcaattgctc ccttaataca
     3121 atgattttac caatcaaccg gtttttcagt atcatcaaag gcttcaattt ttttctgcat
     3181 ggttttgtca aacaaatcaa tggtctcttt caggaaatgc ttgcatatat gatgatatat
     3241 cttgagttcc attactctat ccaatcaaat aaatattgtt cattgtgctc aacttcaaat
     3301 ttgtggagga attcaatgta ttcttccttg aaagttttct ttttgtgatg ctctttttgg
     3361 ttttgaatat atttacaaac atcatcaatt tgagagtgac tgtatgaaaa tgctccaaaa
     3421 ccttcctgcc atgaaaaatt agatagagtg aattttttct cttttatgaa tgcgttggat
     3481 gatttcttta tctctctgac taaatcagac aagcaacagg tgggtttcag accaatgaaa
     3541 aaatggatat gatccgacat tccgttaatg gacaacattt tttgtccctt gtttcgaaca
     3601 atgccggaaa tgtatttgta aagctgttct ccccattcag gtttaataag gctttcccgt
     3661 cctttaactg caaatacggt ttgaatgtat atttgagaat aggttgacat aattagtttt
     3721 ttatttgttg ttcatcccga agggatgtga tgtttataga aaatatctgc aaatacggtt
     3781 tgaatgtata tttgtgaata ggttgacata attagttttt tatttgttgt tcatcccgaa
     3841 gggatgtgat gtttatagaa aatatccaga acagaaacca tacgacccct tcggggtcga
     3901 acgtcaatac ggcatatatt tttctataaa catttgaccc cataggggtc tgtattattc
     3961 caatgtattt cattgattaa aaaatgtcta tgtgaataaa ctgtaagccg ttgccagaat
     4021 gttgaaatca taattgactt caatttgcgt ggcttcgcgc caccttatag caaaaaaatc
     4081 ctctaacata atggatatac aatgatttac aagatttgat tgtagccttg tcaggaatcg
     4141 aacctgaatc ttgggttccg gagacccaca tactatccat tgtactacaa ggccgggaat
     4201 ttcaattcca aaattaaaac cttttgcggg aaatcagaac cttattcata gcgctaatct
     4261 gttgcggacc aacccggcag cagccaccta ttatatccgc tccagcttca agccattctt
     4321 cgactaaatt attgtccagt atatcccttg agttttccca ggatttattc tccgcattaa
     4381 atttttctcc tgaatttgga tacactataa tctttttctc ccaaccttgg tttttgattt
     4441 tttgaataag accttttaca tagcctggcc gggtacaatt taccccggtg gcccaaattc
     4501 ccggaacggc cttgagcatt tctgtacatt ctgctattgg ggtgccatca caaatatgct
     4561 cctcatcccg gcagctaaaa gagatccagg acggcgtggc tgtcgactcc aaaattgccg
     4621 cgattatttt ggcctccgaa aaggaaggaa tagtttcaaa tgccagtaga tcagcgcttc
     4681 cagaatccag taatcttatt ttggcttcat gaaaatcgaa cagttccatt tcatggatag
     4741 tataattacc ccggtattct gatccatcgg ccaggtaagc gccgaaaggt ccaatacttg
     4801 ccgcaacaag ggtcgtttcc tgcctgcaat acttttggga gtaccgttca attgccaccc
     4861 gggcaagctc aacagatttg agaatgagcg cttcggcttc aactttatcc agacctttgt
     4921 ccttaaatcc tttcaaggta gcctggtaac tggatgttgt gataatttta gcgcctgctt
     4981 ccaaatagtt aaaatgcgct tgaattatgg cctcaggatt tgtccgcaga acttctgccg
     5041 tccaaaggga attattcagg ttgcagccca gctcttccaa ttgattggat aatccgccat
     5101 ccagtaatat tgggtattca attatgttcg tttttctgcc caagataaag attgaaaagt
     5161 aactttccaa tcgaatacca gagcctgacc gaatctctaa acatgcattt atgcctcaaa
     5221 taccggattt ttgactaatc tttcacctat cctggttgga gttattttca atttgaaaaa
     5281 ttggagaatc cggtttcatt gccctggaac tacttgtggt tggattatct gatttattgc
     5341 gctacctttg tataataatt gcggattttt taattcactg cctgaaagga ttcattccat
     5401 aatgtagtat tatcatttcc ccaatttcaa atttagagtt tataccaacg gggacacact
     5461 cattatgata acaggcttat tatttctaac tataagaaag caataataac atttatgcgg
     5521 tttccggtgg ctttagtacg cttaacaatt agtaacaata aataaaatga aagaaggaaa
     5581 agtaaagttt tttaattaca gcaaagggtt tggttttatt acaccctctg attcaggtga
     5641 cgatatattc gtacatgaat ccggtttgat tgatcggatt aaagaaaacg ataaagttaa
     5701 atatgaagag gagccgggca agaagggcat gaatgctgtt cgcgttgaag taatcggcta
     5761 gataatattt caatgaatta aggaaaagct attcattttt ttggatagct ttttttcttg
     5821 atcccttctt tagaaccaca tcacatctac taataaaata tttaataaga tcgggcaaaa
     5881 agtctgcctg ttctgcactt tataataaat taacaattga ataaatttca aaaattagga
     5941 ctttcattac cagtaacaga agtccttaat caaattgggt ttgaaacacc aacccctgtt
     6001 caggaaaaag caattccgct tttactaggt aatgatccga ccgactttat cgggttggct
     6061 caaaccggaa ctggaaaaac agcagctttt gggttgccat taatcgatct aattgatcac
     6121 acctcaagtg ctacgcaagt cctcatcatg gcgccgacac gtgaacttgc acaacaaact
     6181 gccaaacagc taaccctgtt ttcagcagga aagaatgagc taaatattgt ggtcgcttat
     6241 ggaggagctg caattagtaa tcaaataaaa cttctgaaga aacctgctca catcgtggtg
     6301 gcaactcccg ggcgtctcca ggatcttata gatcgaaaag cggtgcgtct aaacaatgtg
     6361 cgctatgtcg ttcttgatga agctgatgag atgctaaata tgggtttcaa ggaagatatt
     6421 gacaagatac tttcctatac gccgaaagat aaagtaacct ggcttttttc ggcaaccatg
     6481 ccggcggaaa tcaggaagat cgtaaacaag tatatggata agccgatcga aatttccatc
     6541 aatacagccg agctcattaa taaagatatt tcccacaaat acgtagtgac tacatcgtcc
     6601 aacaaaatac cggcactaag gcggtttttg gatattcagc ctgacatgag gggaataatg
     6661 ttttgcagaa ccaggcgcga aactcagcat atttccgatg aactgactaa cctgggttat
     6721 ggggttgaag ctttgcatgg tgatttgtcg cagggacagc gtgatgcggt gatgaagaga
     6781 ttcaaaatga gatccatgca attactaatt gcaaccgatg tcgcggcacg tggcattgat
     6841 gttaacgacc ttacccatgt aatgcatcat acacttcctg atcaaccgga tatatatacg
     6901 catcgcagcg gaagaacggc ccgcgcgggt aagaaaggaa tctcgctcgc gtttattaat
     6961 cccagggaag gtaagagaat ctctgaattg gaaagacgaa tgaaagttaa gttcgaacga
     7021 attgaggtac cggcagtaga agaaatgcga tacagcagga taaacagctg ggcaaatctt
     7081 attatcaaca cacatgttga tgataaggcg gaagaagtac ttcaacacct tcatggccaa
     7141 tttgcgcact tatccaaaga agacatcctg cgaagattaa taacaaccca gctcgaccat
     7201 ttaatttttc agcacaatga tgaagatttg aatgaaaagt ctgatgccgg cgccagaaat
     7261 ggtaaaaagg atgcggcttc aaaccggtat tttgttaaca tcggaattat tgatgggatg
     7321 actaaaggag atttgatcca tttcttatct gacgtttcta aggttgaccg gaaacatttt
     7381 ggagaaataa gtatgcacaa gaattattct ttttttcaga taagctcggc tgtggataag
     7441 aaaataagcg atagttttat gggagttgaa attgaaggcc ggcaaatccg ggtgaatctt
     7501 gatgagaata aacgatccgg aagagattct tccggaggaa aaggtaggat gtccggaaag
     7561 cggagttgga attcaaaatc atttaaacga aacattaaaa ggaggtaatt tatgggaaga
     7621 caaaatcaga atgcttttat aaaaaggaag aaggaagagg aaaaacgcaa aaagaaaaaa
     7681 gagaagcaag agaaaaaaga agcgagaaag caagagcctt caagcagtaa attggaagat
     7741 atgcttgcct acgtcgataa agacggaaat ataaccaacc aacctcctga aggttagcat
     7801 agcattatac tttaggtata atatacataa tagtaaattc ctagctgtga tccagatatc
     7861 gaagtactac ggtttcatct ttagacaacc cttgtatctt tctggcttta ccagatgaat
     7921 tccattttgg ccggaacatt ccttcatgcc aatctccaag aactaccgga tgaatataag
     7981 aagatctgca aaccgcaacg gtattgccaa ggtctgaagc aacagtttta ataacctggg
     8041 tcgccacctt ctttcgcgcg gtgatggtat ccggatcaat ctggagtgat agacgtgaag
     8101 ctgctttcca tgtcgcagcc cacgtcctga aatctttggc tgttactttt ttatcggagg
     8161 gaacaagcga atcgagatac tcattgatat gataagattt gatatcaaaa tcttttcccg
     8221 ttgagtcttc atatctaaat aaatcctgcg atttatgtct gccacctacc ttaccggatt
     8281 cgtcaattaa gatggctaaa tcatcctccc aaattctgat gttccaatcc ttacctgatt
     8341 ttccccggaa ttcaagtata gcatcaagct cgccttcagc cgttttactc cggtcaattc
     8401 tcatgtggcc ttctttaagc gtagttaagc catagctatt atttttcctg gcatattcat
     8461 catttccaac tctgatgtga tacagatcca ttaacttaat aaccagtgcg actactttat
     8521 cttttggcat accagtaaga gaaagatcac gatcaatctt ctttctaata tcgggcagga
     8581 gagaagcaaa cattgtcaga ctctcaaatt tttcagcgct tctgaattcg gtccacgagg
     8641 catgatatcg atactgaagt ctacctttgg catccttacc tgtagcctgc aaatcacttt
     8701 taggatcgtg agaaatccat acatcctccc atgccggtgg gatgaccaat ttattgcatc
     8761 gatcaattat ttggagattc gtaatccgct ttcctttccg gtcaaaataa tcccaaccca
     8821 ataccttttc actttccctt attttttttc gccgcttgcg tgtgatccct gttttgattg
     8881 aacccatgtt tgttgagata tatttttaac acaagtatgc agtatacaat atactgctac
     8941 tttcttatta aatgcaatgg gtattcgtat ttacctggaa atgagactct atttttcact
     9001 aaattaatcc ctgaaaaaaa caagacaggt tagatgcaat tacccatact tcaggatatt
     9061 gtaatcattc tcgggttatc agtagttata attcttctct tccagaaact caggctgccg
     9121 gcaattctgg gttttctgtt aaccggtatt attgcgggac ctcatggttt gaatttaatc
     9181 agcgcaacac atgaagtaga actgctgtcc gagattggaa tcatttttct tttgtttgtt
     9241 attggaattg aattctccct gaagggattg tcaactgtta agaacaccgt acttataggt
     9301 gggctgtttc aggttggtgg taccattgca ctaacggcat tgttttccta tatgctcggc
     9361 atgactatac catctgcaat ttttatggga tttcttattt cacttagcag tactgccatt
     9421 gtattgaagt tgctgcagga gcagggcgaa cttacatcac ctcatggccg gatttcagta
     9481 gccatactta tttttcagga tattattgtg gttttgatga tgttgcttac accgctactt
     9541 gccggtgaga tggaaaatcc aggaacaact ctggctattc ttgttgttaa agttgtggga
     9601 gtgattgtgg tagttattgc tctggctcgc tacatagtgc ctaacctctt tactttggtg
     9661 gtcaaatcca agagtcagga attatttatt ctcactgtgg tagtgatatg ctttgccact
     9721 gcctggctca cctcttcaat tggtctttcc ctggcattag gtgcgttttt tgccggactc
     9781 ataatttctg aatcttctta cagccatcag gccactgcta atattcttcc atttcgcgag
     9841 attttcatca gctttttctt tgtgtctgta ggcatgttgc ttgacttgtc attctttatt
     9901 gaccatgtag taaatatact gctgttggcc attggagtta ttttcctaaa aataattgtg
     9961 attgcaatta ctgttctgat tttgaattat ccaccacgga caataattct taccgcgctt
    10021 tctctttttc aggtagggga gtttgcattc ttactttcca cgagtggaat gaaatacaat
    10081 cttctttccc aggaatttta ccaatacttt ctcgcgattt ccatcattac aattgggatc
    10141 acgcccttta tcatgaagtt ttcatcctcc attgctcaat ttattttgca tgcgccactg
    10201 ccgggccgcg tgaaaaggcg acttaaagtg ctgactcacc ataaagcgga aaccaccgcc
    10261 aagcatgaag atgagttaaa ggatcatctg gttattattg gttatggaat taatggagaa
    10321 aatgttgcca aaactgcacg ccaggcaaaa attccctatg ttattgtcga acttgatccc
    10381 gtagctatca acaaagcaaa cagaaacaag gagcccgttg tttttggaga cgcatccaac
    10441 gatgtcatcc tgaaacacgt tcatattcag gaggccaggg taatagttat tgcaattagt
    10501 tctcccgaat caacaaaacg aatcatgcgt cgcgttcggg agtttactaa aacggccttt
    10561 gtaatagtgc gcacgcgata tgtaagggaa gtggaggaaa ttgtcggatt aggtgcggat
    10621 gtggttatcc cggaagagtt tgaaacctcc attgaaattt tcacacgggt attgaagaaa
    10681 tacctgatgc ctaataatga gataatggaa tttgttaatc agatccggtc tcacaattac
    10741 gaaatgctgc gaagtattcc cggattcagt gtagtaactc aggcaaatat acccgatatg
    10801 gaaattgcaa ctctgccggt acaccagggc gtcaataaag ttgttggaaa aagcgttcag
    10861 gaatccggaa tccggagtaa tttcaacatc accgttctgg ctatcaaaag gggtgagaag
    10921 tatatcacca gggttacacc ggagaccata atcacccagg atgatattct ctttttgttc
    10981 ggaagtccga attccatcat cgcgttaaac aagttcctga tgcttaatta agatttctga
    11041 atccggagat ttattcgtct ttcaggctat ctaccggatt tgccaaagcc gtttgaatgg
    11101 ctttaaaact cacagtagca accgcaataa taagtgcgcc cagtcctgcc gcaataatta
    11161 ttcccgggcc gattgatatt ctaaaagtaa attgctccag ccaattattg gagaagtaga
    11221 tgccaaccgg tgcggcgaga ataaaggcaa ttattatcag ctttccaaaa ttcagggaca
    11281 atagcataaa caggttagtt atactggctc ctaaaacttt tcgaatgccg atttccttaa
    11341 cccgctgctg ggcgataaat gatgatagtc caaacaatcc caggcaggaa ataaaaatag
    11401 caattacggt aaagtagctg gccagatcgg ctatcatcat ttcatcctta taatcctgct
    11461 caaattgatc atccaggaag gtgtactgaa aggggtatcc gggattaagc cggcgtgtta
    11521 ctttttcaat ttcctggaca gtttgttgta tgtttccact aatgcggata aaggcaagcc
    11581 ttccgattgc atttgacctg ataataagcg gttcaattgc ttcatgaaga ctctccatat
    11641 gaaaatttct taccacacca ataattttac cattgatacc ccataaggag aaattttcac
    11701 cgaccggatt gtccttattc attaggcgtg ccagctgttc attaataata aaagaggtag
    11761 agtcccggta aaaatctcca ctaaaatcgc taccacttat caattccatt ttcatggtct
    11821 taagaaaatt atggtctgtt gacaaaacgg atatttctat ttgaaagctg gggtcttttc
    11881 catcccaatt ggccccgcct gtagaccgtc ccactcccaa gggattctgg tcggaactgg
    11941 ctacgctgga gatgctggga attcgtaata attctgtacg gtatgcatca aagttttgca
    12001 ggatagctcc ttcaattgga acatagataa tgttctctct gtcaaggcca agatttttat
    12061 caagaataaa catgatttgc ctgaatatgg aaacagaccc gataatgagt acaatggaaa
    12121 tagcaaattg aaatactacc agagctttgc ggagtgttgc acctccgcca accttaataa
    12181 tgtgacctct aatcacatgg atagtattaa taccggaaag gaaaaatgcc ggatacattc
    12241 ctgatagtaa acctgtaacc aaagcaatcc ccgtgatcat caaccaataa gtaggggcgc
    12301 tgaaatcaat ttgaataaac ttaccggtga gttggttgaa acccgggagg attagttgaa
    12361 caattaaaat agcaaaaaat gatgctgtga aagctattgc cagcgcttca gttaaaaatt
    12421 gaagcgcaag tgaattgcgg cctgcgccca taacttttct cactcctatt tctttaactt
    12481 taatggcaga ccttgctgtt gctaaattca tgaagtttat gcaggcaatg ataataatgc
    12541 agatggctac aatcagcaat gcccgaacgt aatcaatcct tcctccctgt gctgcaccat
    12601 tttcaaaatt ggaatacaag tattgatctg aaaatttctg gtatactatg cgctcatcac
    12661 cgccctgaat attattgttt acttcattca atactttttc accaaaagca ttaatatctg
    12721 cgccgtcttt cattaatacc accatttgga atccgccatt attccaattt tccacccagg
    12781 agttttctga aatatatcgc tgcgcgggga tcaaccattg gaattgtaag gaagaatgag
    12841 gagaaggatt ttcaaacaca ccggaaattg taaaagtatt attacccaga ggaagggtag
    12901 accctaaaat gtttgattgc caattttcac cgtataattt agccgcgaga tcttcagaaa
    12961 ttacaatgga gttaatttca ttcaacgcgt tttccttact tcctataata agaggaaaag
    13021 aaaaaatctt caaaaactcc ggtgatacat accggcccgt ttctttaaaa gatcgatctt
    13081 caagtttaaa taaagcttca atatcccagc caatcaaggt caccatatca atttccggat
    13141 aggtttcttc cagagcaatt tccagaggct gaggaattgc ttcggtatta aaattctgtc
    13201 catcatcctg gtaaataatt cttttcagct gataaatatt agctgacttt tcatgaaaac
    13261 cgtcaactct gtattcatct tcaacccaaa gtaaaatcag aaaactgctg gcaaggccaa
    13321 cagtcagtcc aagggtatta atgaatgtgt aagatttttg cttaagcaaa cttctaaatg
    13381 ttgttttgat gatgtttctg aacatagcgg gtagcattaa agttgaactt gaatatttgt
    13441 gattgtatga aatt
//
LOCUS       DASWHX010000176         9098 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_185407, whole genome shotgun sequence.
ACCESSION   DASWHX010000176 DASWHX010000000
VERSION     DASWHX010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 9098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9098
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185407"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            143..1861
                     /locus_tag="VGA21_11530"
     CDS             143..1861
                     /locus_tag="VGA21_11530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018479714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HEX9651184.1"
                     /translation="MSEIDWIVLSVTIGFIVTIGVYKNRKSQSLSSYLLSDKKLNWRA
                     VAISIIATQASAITFLSMPGQAYSDGLGFIQIYFGLPLAMLVLAYIIMPLYKRLNIYT
                     AYEFLEMRFDLKTRVLGSILFLIQRSLAVGFTIYAPSLIFSNILGWNSLLTSLLIGVL
                     IIIYTVSGGAKAVMTTHRHQMLIILIGMFVVGGFALFKLPVGVRFQDTFLVADTFGKL
                     NPVSLNFDITERYNIWSGLIGGFFLALAYFGTDQSQVQRYLSGKTTQAAKRGLLFNGI
                     IKIPLQLAILFLGILVFTFYQFEKPPIFFNQKQYQKVAESEYGLDLTYLESVHNELFI
                     QKKEKLKHLLDNSSNNLTTELITIELQSIEKRIDEVESEAARLIALNSPGTTTNDKDQ
                     IFLTFITNHLPIGMVGFLLAVIISASMSSASSILNALAGTSIVDIYQRLYKSDKKDDH
                     YVSAGRKFTLLWGLAAIGFAIFANFTENLIQAINIVGSLFYGSVLGIFLVGFFFQKIR
                     GNAVFIAAICSQVLVFILYLGTDISFLWFNLIGTLFVPLIGIILQITFYKDDKPFHPR
                     GYASRG"
     gene            2120..3031
                     /locus_tag="VGA21_11535"
     CDS             2120..3031
                     /locus_tag="VGA21_11535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651185.1"
                     /translation="MREPQFLKFLEAWHQIMIILALGAIVVAILFFVYHKLRSSAFKQ
                     PKAKYDYLKLHEIRYYWYSVLSISIAIGMVANTYDDETVSLSIVWFFVRCFISIAIAT
                     LFGYVIYLILKFYYPTKLQRKLMKLRYQPRISKTGKEMQLLSEEEEDVHLDEGRQAEE
                     NVFSIDYDVWVDTETGEVKIEKYAGELEALRCNTCGFQTMQLNREEIIKSATKEEAGE
                     LLKYYQCAYCKSRRTTSHRISPIIDSMDQFKLPLGASYVGERKVKSIMIQIVDTKGEL
                     REFFFQNTAQARQFLEEFDFDRLPVEE"
     gene            3153..5237
                     /locus_tag="VGA21_11540"
     CDS             3153..5237
                     /locus_tag="VGA21_11540"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HEX9651186.1"
                     /translation="MKYVVGLIIVTLVSFIGIKTYLPSEEHLNESPQFIVLTSGDTSK
                     ILKPEAHYGREAQLVTQLLQQYHFRKTSVDDSLSAIIFKDYIESYDNNKVYFLKSDLD
                     TFEKFRFRIDDDLKAGRLEFPYQLFEVFKARFFEKNDFVMELLGQDFDFSLDEYYDSD
                     RTKAEWPRTENEQNEVWRKILKAQLLNHKLTGKDIAESKEILRKRYEGYRKAIAQYSS
                     EDIFQQYLNSFTQSYDPHTNYFSPVSAQSFMIDLNLSLEGIGARLTTDGDYTIIANVV
                     AGGPAFKSNMIHENDKITGVAQGDEEKFVDVIGWRVTDVVKLIRGPKGSIVRLQVIPA
                     GEATNAIPIIVRLIREKIQLEDEKVTSEVIPINKNGKDYKLGVITVPSFYFDYDAYRK
                     GDMDFNSTSRDVRKILGTFNEEAVNGVLIDLRRNGGGSLQEAIDLTGLFIKDGPVVQV
                     RSRNGSVEQYEDTDRNVVWEGPLAVLTNRFSASASEIFSGAIQDYQRGVIIGEQTYGK
                     GTVQNMIDLSRFMELGEGKAGEVKLTQAKFYRINGNSTQLKGVTPDVNLPSAYDASIF
                     GEGSKPTALKYDEIQSAKFSKTGKVDSDLIARLNNSYLERLENDPELKGLQEEIKESN
                     ENRNNTVQSLKEDIRKKELEEMEQRRAARAKLSGAIVNPESKNMKLVEMHDPYLKEGL
                     LILAELVSLNIG"
     gene            5243..6127
                     /locus_tag="VGA21_11545"
     CDS             5243..6127
                     /locus_tag="VGA21_11545"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEX9651187.1"
                     /translation="MKFGRVSAEELDQLELSLPSDHKSIRELSGKKSKAPKVYIGCAK
                     WGRKEWLGLLYPEGTSEKDFLNNYVNHFNAIELNGTFYGTKKAHIEKWAESGVKGFKF
                     CPKFTQIISHRKRLKEAEQITEYYLDAINAFGDYLGKAFLQLPENFGPKNFDIFKSYI
                     QSLPGDAPVTVELRHKGWFEDKTIFDETVSMLRENNKGIVITDVAGRRDALHMAITTP
                     YTFIRFNGYDLHQSDYSRMDEWALRLKSWLESGLEEIYFFLHQEDERHTPETSSYFTG
                     KINEVCNLDLRLPNFLNQ"
     gene            complement(6131..6985)
                     /locus_tag="VGA21_11550"
     CDS             complement(6131..6985)
                     /locus_tag="VGA21_11550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651188.1"
                     /translation="MFLLRIIAYTHIIVLLTIGFEASAQRKNDDPKHENHYRKVKAES
                     PEFTIEITEAHSQQEFTLVKMQITNKTDDFLYFKGAEPLLRYEFGEYHPTGGGGLFKG
                     LNIVIDPHSSESQTLKVTGGNNFHVEKFSVLMDGFYRVSANGKVQEGEPFQLPPSVND
                     FKAGSFSCNLDKIKKETKETSVTFKFQYQGTQMGILQPGKITLKLENGQEYANDNRKE
                     RNQLLHSGENGKFNATFHVPGKVTDMQFANMQLIWKSTFSESEMIKLEPLSIDLELDP
                     GLTEGKNK"
     gene            7063..7359
                     /locus_tag="VGA21_11555"
     CDS             7063..7359
                     /locus_tag="VGA21_11555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651189.1"
                     /translation="MTQFSELFYNLSLYLLILSTLLSFFLGSVDIRFLGSKTLWHLIR
                     RYVVVSLLTMVILSAMLVAAGLLVTLDFSLLFTSLGLNLASVLIFFLFTNPAEK"
     gene            7405..8520
                     /locus_tag="VGA21_11560"
     CDS             7405..8520
                     /locus_tag="VGA21_11560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008198112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HEX9651190.1"
                     /translation="MKKTVLFFLIYGFGLTVANSFQRAADLGIKIGILNKGSFNSITD
                     VEGVMVGQVTIQNDSVNTGVTAILPHGDNLFTNKVPAAIYVGNGFGKLAGITQVNELG
                     NIETPIILTNTLNVSTAVSGVVSYILNLPGNENVRSVNAVVGETNDGGLNDIRGRHVT
                     ESHVLEAISLAKTGPVDEGTVGAGTGTICFGFKGGIGTSSRVLPQTVGGYTVGVLVQT
                     NFGGVLEINGAPVGQELGKYYLQDAVDNNPDGSCMIVVATDAPVTNRNLERIAKRAIF
                     GIARTGGIASNGSGDYVIAFSTNKELRLVNDNSNLRSFIELDNNAMSPLFLAAIEATE
                     EAIVNSLLMATVIPNTNIPELPTDKLMDILRKYGKVK"
     gene            8570..8932
                     /locus_tag="VGA21_11565"
     CDS             8570..8932
                     /locus_tag="VGA21_11565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651191.1"
                     /translation="MDENELFSFYKWITNIGLVLFVAFSYFFFGPVVELQSDINNLHE
                     QIEIDNATIDNTTNNATKIQLIKANLARAENAGSKTMTLNITRIGSLLFMAVGLGAMV
                     YGFVNIRKIISSADSGEI"
BASE COUNT         2787 a         1636 c         1860 g         2815 t
ORIGIN      
        1 tttttaccca aaacattatt aaagtaattt gtgaaagtga agaccttaaa ctgctaataa
       61 tgtattggtt atggaattct aataataatg caataactca attccctcac gtttcgtaaa
      121 gctttaaggg caaacttggt aaatgtcaga gattgattgg attgtcttat cggtaacaat
      181 tgggtttata gtaaccatag gggtttataa aaatcgaaaa tcacaaagct taagtagcta
      241 tttattaagt gataaaaaac tcaattggag ggcagtagcc atttctatta tagccaccca
      301 ggccagcgcg attacttttc tttccatgcc gggtcaggct tatagtgatg gacttgggtt
      361 tattcaaatt tacttcgggc tgccgctggc aatgttggtt ctggcttaca taataatgcc
      421 cctgtataaa aggttgaata tctacaccgc gtatgaattt ttggaaatgc ggtttgatct
      481 taaaacccgg gttctcggct ctattctctt tttaattcag cggagtcttg ctgttggatt
      541 taccatctac gctccttccc ttatattttc gaacattctc ggttggaata gcctgctgac
      601 cagcctgctt attggtgtac tcataattat ttatacggta tccggcggtg ccaaagctgt
      661 catgactact cacaggcatc aaatgctgat tattttaatt ggaatgttcg ttgtaggggg
      721 ttttgctctt ttcaaattgc ctgtgggggt caggtttcag gacacttttt tagtagctga
      781 tacatttgga aaattaaacc ctgtgagctt aaattttgac atcacggaac gttataatat
      841 ctggtccggc ctgattggag gtttttttct ggctttagca tatttcggaa ctgatcaatc
      901 ccaggttcag cgatatctat cagggaaaac aactcaagca gctaagagag gtctgctttt
      961 caatggtatt atcaaaattc cacttcaact ggcaattctc ttcctcggaa ttcttgtttt
     1021 taccttctat cagttcgaaa aacctccaat tttctttaat caaaaacaat atcaaaaggt
     1081 agctgaaagt gaatatggtc ttgacctcac ttaccttgaa tccgtacata acgaactttt
     1141 cattcaaaaa aaagaaaagc ttaaacacct cctggataac tcaagtaaca atttaactac
     1201 cgaattaata accattgagc tacagtcaat tgaaaaaaga attgatgaag tcgaaagtga
     1261 ggcggcaaga ttgattgccc tgaatagtcc tggtactacg accaatgata aggatcaaat
     1321 cttcttaact tttataacga accatttacc tataggcatg gtcggattcc ttttagcggt
     1381 gatcatttcc gcttcaatgt cgtcagcctc ttctattttg aatgctctgg caggaacttc
     1441 tattgtagac atctatcaac gcctgtataa gtctgataaa aaagacgacc attatgtttc
     1501 tgcaggaagg aaattcacac tcttatgggg cttggccgcc atcggatttg ccatttttgc
     1561 caatttcacg gaaaacctga ttcaagccat taatattgtt gggtctttgt tttatggatc
     1621 tgtgcttggt attttcctgg taggcttttt cttccaaaaa attagaggta atgctgtttt
     1681 tattgcggcg atatgctctc aggtgcttgt gtttatactc tacctgggca cggatatttc
     1741 atttttgtgg tttaatctta tcggcactct ctttgtcccc ttaattggta ttattctcca
     1801 gataactttt tataaagatg ataaaccatt tcatccgcga ggatatgcat cgcgaggtta
     1861 atctggcagg gtattcttgg taacccaata tctataccca attattacct tatccagagc
     1921 agataattga tttaagtctt tcttgctgta tctgggaagg ataatcttat ttatcctggc
     1981 caacattcta aataaattcc gggctaaatt ctgcattcta ttgaaataat aaattggata
     2041 ttttcttata ttaacaaagg gaacaatatt tgcaatatat taaagtaata ttcatattga
     2101 acgaaaaatc acaggttaga tgagggaacc acaatttctt aaattcctgg aagcatggca
     2161 tcagataatg ataattctgg ctctcggagc aattgtagtg gcaattttgt tttttgtata
     2221 tcataaatta agaagctcgg cttttaagca gcctaaggca aaatacgact atctgaagtt
     2281 acatgaaata agatactatt ggtattcggt tttatccata tcaattgcaa tcggcatggt
     2341 tgccaatact tatgatgatg aaactgtaag cttaagcatc gtctggttct ttgttcgctg
     2401 ctttatcagt attgctattg ctactttgtt cgggtatgtc atctacctca ttctaaagtt
     2461 ctattatcct acaaaacttc aacgaaaact catgaagctt cggtatcaac ccagaatctc
     2521 gaagactgga aaagagatgc aattattaag tgaagaggag gaagatgtcc atctggatga
     2581 gggaaggcaa gccgaagaaa atgtattttc tattgattat gatgtgtggg ttgacactga
     2641 gaccggggaa gtcaaaattg aaaagtatgc tggagaactt gaagcattac gatgcaacac
     2701 atgcggtttc caaaccatgc agttgaaccg tgaagaaata atcaaatcgg ccacaaagga
     2761 agaggccggt gaactgctca aatattacca gtgtgcatac tgcaaaagca gacgaacaac
     2821 ctctcaccgt atatcaccaa tcatagactc gatggatcag ttcaaactgc cattaggagc
     2881 atcatatgtt ggcgagcgaa aagtaaaatc cataatgatt cagattgtgg atacaaaggg
     2941 agaattgcgg gaattctttt ttcagaatac cgctcaggcc aggcaattcc ttgaagaatt
     3001 tgattttgac agacttccgg tagaggaata atttttccat ttttttaaat accgtactct
     3061 acaaaaatgg cctttgtgga ataaaattgg gtaaaaccca tcgttttgaa ttaattttcc
     3121 gtatagaatg aatataattt aagtgaattg acatgaaata tgtagttggg ttaatcattg
     3181 ttacactggt ctcttttatt gggataaaga cctaccttcc gtctgaagaa cacttgaatg
     3241 agtcaccgca atttattgtc cttacaagtg gagatacaag taaaatcctc aagccggaag
     3301 ctcactatgg cagggaagct caattggtta ctcagctcct acaacagtat cattttagaa
     3361 aaacttcagt ggatgattcc ttgtcggcca taattttcaa agattatatc gaatcctatg
     3421 ataataacaa agtttacttt ctaaaatcag accttgacac tttcgaaaaa ttcaggttcc
     3481 ggattgatga tgacttaaaa gccggccggc tggaatttcc ttatcagctt ttcgaagttt
     3541 ttaaagccag gtttttcgaa aagaacgatt ttgttatgga gttacttggg caagactttg
     3601 atttttccct ggatgaatat tatgattcag acagaaccaa agcagaatgg cctcgaactg
     3661 aaaatgaaca aaatgaagtg tggagaaaaa ttctcaaagc tcagttgctt aatcataaat
     3721 tgacagggaa ggacattgcg gaatcaaagg aaatacttag aaaacgctac gagggatatc
     3781 gcaaagcaat tgcacaatat tccagtgaag atatttttca gcagtatttg aactccttca
     3841 cacaatccta tgatccgcat accaactatt tttctcccgt atcggctcaa agctttatga
     3901 ttgatttgaa tctctctttg gaaggaatag gtgcccggtt aacaaccgat ggagattaca
     3961 ccattattgc caatgttgtt gccggtgggc ctgcttttaa aagcaatatg attcatgaaa
     4021 acgacaaaat taccggagtt gcccagggag atgaagagaa atttgtcgat gtaattggct
     4081 ggcgggtgac cgatgttgta aaactaataa ggggacccaa aggatcaatt gtccgattgc
     4141 aggtaattcc cgccggagaa gctactaatg ctattccaat cattgtccgc ctgattaggg
     4201 aaaaaattca gctcgaggat gaaaaagtta ccagcgaagt tatccctatt aataaaaatg
     4261 gaaaggatta taaacttggt gtaattactg ttccttcttt ttattttgat tatgatgctt
     4321 accggaaggg ggatatggat tttaatagca caagccggga tgtgcggaaa attcttggga
     4381 catttaatga ggaagctgta aatggtgttt tgatagatct acgaagaaat ggcggaggca
     4441 gtttgcagga agccatagat cttacaggac tatttattaa ggatggacca gtcgtacaag
     4501 tacgatcacg aaatggaagt gttgagcaat atgaagatac agacagaaat gtggtgtggg
     4561 aaggcccatt agcagttctt accaacaggt ttagtgcttc agcttcagaa attttttctg
     4621 gcgccattca ggattaccag agaggggtca taattggtga gcaaacctat ggcaagggta
     4681 ccgttcaaaa tatgatagat ctctcaaggt tcatggaatt gggagaaggc aaagcaggag
     4741 aggtcaaact tacccaggct aaattttata gaattaacgg aaacagcaca caactgaaag
     4801 gcgttactcc ggacgtaaat cttccctctg cctatgatgc atcaatcttt ggtgaaggca
     4861 gtaaacccac cgcattgaaa tatgacgaga ttcaatccgc gaaattttcg aaaactggta
     4921 aagttgattc agacctaatc gccaggctga ataatagtta cctcgaaagg cttgaaaatg
     4981 atcctgaatt aaagggatta caggaagaaa ttaaagaatc caatgagaat cgtaataata
     5041 cggttcaatc ccttaaggaa gatataagaa agaaggagtt ggaggagatg gaacaacgaa
     5101 gagcggccag ggctaaactt tccggagcta ttgtaaaccc cgaatccaag aatatgaaat
     5161 tagttgaaat gcatgatcct tatttaaagg aaggacttct tatattggca gaactggttt
     5221 cattaaatat tggataaaac tcatgaagtt tggaagagtg tcagctgaag aacttgatca
     5281 gcttgagctt tcgttaccat cagatcacaa aagtatccgt gaactttcag gaaagaaaag
     5341 taaagctcca aaagtctata taggatgcgc aaaatggggc aggaaagagt ggttgggttt
     5401 gctttatcct gaaggaacct ctgaaaaaga ctttctgaat aattatgtca accatttcaa
     5461 cgccattgaa ttaaacggaa ccttttatgg aaccaaaaaa gcacatattg aaaaatgggc
     5521 ggaatctgga gtaaaaggat tcaaattttg cccaaaattc acccagatta tcagccatag
     5581 aaaaagactg aaagaagctg aacaaataac tgaatattac ctggacgcga taaatgcttt
     5641 tggggattat cttgggaaag catttctgca attgccggag aatttcggcc ccaaaaattt
     5701 cgatattttc aaaagttata ttcagtcatt gcccggggat gccccagtga ccgttgaatt
     5761 aagacataaa ggatggtttg aagacaaaac tatttttgat gaaaccgttt ctatgcttcg
     5821 tgaaaataat aagggaattg taattactga tgttgcgggc aggagggatg ccttgcatat
     5881 ggcaataact actccatata cttttatcag gtttaatggc tacgatttac atcagtccga
     5941 ttattccaga atggatgaat gggccttgag attaaagtcc tggctggaat ccggattaga
     6001 ggaaatttat ttctttcttc atcaggaaga cgaaagacac actccggaaa cgtcatcata
     6061 ttttacaggt aaaatcaatg aagtatgtaa ccttgacctg cgtctcccca acttcctgaa
     6121 tcaataaata ttacttgttc tttccttcag ttaaacctgg atctaattca aggtcaatcg
     6181 atagtggctc aagtttgatc atttcagatt ccgagaatgt gcttttccag attaattgca
     6241 tgtttgcaaa ctgcatgtca gtaaccttgc caggtacatg aaatgtggcg ttaaattttc
     6301 cattttcgcc gctgtgcagt aattggtttc tctctttgcg gttatcgttg gcatactcct
     6361 ggccattttc aagtttcaaa gtaattttac ccggctgcag tattcccatt tgagttccct
     6421 gatattgaaa tttaaaagtt actgaagtct ctttggtttc tttcttaatc ttatccaaat
     6481 tacaactgaa tgaaccggcc ttaaagtcat taacagacgg aggcagttga aagggctcac
     6541 cttcctggac ttttccattt gccgacacac ggtaaaagcc atccatcaga acagagaatt
     6601 tttccacatg gaaattattt ccgcctgtta cctttagagt ttgtgattcc gatgagtgcg
     6661 gatctataac aatatttaat cccttaaaca atccacctcc gcctgttgga tgatattctc
     6721 caaattcata tctgagtagt ggttctgccc ccttaaagta cagaaagtca tcggttttat
     6781 tggttatctg cattttaact aaagtaaatt cctgctggga gtgggcttca gtaatctcga
     6841 tagtaaactc aggagactct gcctttacct ttctgtaatg attttcatgc tttggatcat
     6901 catttttgcg ctgggctgat gcctcaaatc caatggtcaa aagaacgata atatgcgtgt
     6961 aagcgattat gcgtaataaa aacatataaa taatagttta atgtaaaatg atatacctat
     7021 aactttgtaa aacacaaata tataattata aaatataata aaatgactca attttcagaa
     7081 ttgttttaca acctgagcct atatctgctt atcctgagta ctttgttgtc tttctttctg
     7141 ggttcggtag atattaggtt tttgggctca aaaactcttt ggcaccttat ccgaaggtat
     7201 gtagtagttt ctttgttgac tatggtgatt ctttcagcaa tgctagttgc tgccggcttg
     7261 ttggttacac ttgatttcag cttgcttttt acaagcctcg gcttaaacct ggcttccgtc
     7321 cttattttct tcctattcac aaatcctgca gaaaaatagc cggcgaattt ttcatttatc
     7381 ttcaattctc tatttttaga gagaatgaag aagacagtgt tgtttttttt aatttatggg
     7441 ttcggattaa ccgttgccaa ttcctttcag cgtgctgcag atttaggtat aaaaattgga
     7501 attttaaata agggctcatt caattcaata acagatgtag aaggagttat ggtcgggcag
     7561 gttactatac aaaatgatag tgttaataca ggagtaaccg ctatactccc tcatggggat
     7621 aatctattta ccaacaaggt tcctgctgca atttatgttg gcaatggatt tggtaagctg
     7681 gcaggaataa cgcaggttaa tgagcttggt aacattgaaa ctcccattat acttactaac
     7741 accctaaatg tttccactgc tgtttccgga gttgtcagct atattttgaa tttacctggt
     7801 aatgaaaatg taaggtcagt aaatgctgta gtaggtgaaa ctaacgatgg cgggttaaat
     7861 gatatccggg ggagacacgt taccgaatct catgttttag aagcaattag cctggccaaa
     7921 accgggccgg ttgacgaagg tacagtggga gcgggaaccg gcacaatctg ctttgggttt
     7981 aaagggggaa tcggaacaag ttccagagtt cttccgcaaa cggtgggcgg atataccgtt
     8041 ggggtgctgg ttcaaaccaa cttcggtggc gttctggaaa ttaatggtgc gcctgttgga
     8101 caagagctgg gaaaatatta tttgcaagat gcggttgata ataatccgga tggttcctgc
     8161 atgatcgttg ttgcaactga tgcacctgtt acgaaccgga atcttgaacg gattgccaaa
     8221 agagcgatct ttggcattgc cagaacaggt ggaattgcct ccaatggaag tggtgattat
     8281 gtgattgcct tcagtactaa caaggaatta aggcttgtaa atgataattc caatttaaga
     8341 agttttatag aactggataa taacgcgatg tctccattgt ttcttgccgc aattgaagcc
     8401 actgaagaag ctatcgtgaa ttcgttatta atggctaccg taatccccaa taccaatata
     8461 ccggaattac ccacggataa actgatggat attctaagaa aatatgggaa ggttaagtaa
     8521 atttggccga ataattcaat aaacataaat cataacacat aggtgtaata tggacgagaa
     8581 tgaactgttt tctttctata agtggataac caacatagga ttggtccttt ttgttgcttt
     8641 ttcttatttc ttttttggcc ctgttgttga attgcagagc gacataaata atttacatga
     8701 acaaattgaa attgataatg caactattga caacacaaca aataacgcta ccaaaattca
     8761 gctgattaaa gccaaccttg ccagagccga aaatgccggc agtaaaacta tgacgcttaa
     8821 tattacgagg attggctctt tattgttcat ggcggttggg ctgggcgcaa tggtatatgg
     8881 cttcgtaaat atcagaaaaa taatttcatc tgccgactcg ggggaaattt aacattagcc
     8941 gcctctcctt aaactcttaa tttcgagttt ggtattcttt ttcatctaat tttttgagac
     9001 ctggaaggaa attttttctt taataaattt ctgatattat cctggacatc gaaggtcact
     9061 cttcaaagaa attgcagaaa ttcttaacca tagtttat
//
LOCUS       DASWHX010000177        11309 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_1686165, whole genome shotgun sequence.
ACCESSION   DASWHX010000177 DASWHX010000000
VERSION     DASWHX010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 11309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11309
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1686165"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..688)
                     /locus_tag="VGA21_11570"
     CDS             complement(<1..688)
                     /locus_tag="VGA21_11570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651192.1"
                     /translation="MRTLLTLSYSDLCSTFREPIFRLLIFFPFFSFAIIRWGYPAILN
                     LYPPIEPYSQVILMWACLQSATLFGFIYGFIFLEEKEENIWQAIQVLPISGFNLVTSR
                     LLVGILISTLVNFTLIHWGGIEHFEIWKEIIVALHFSFTAPLIALYIGAFARNKIEGL
                     AQIKVINILVLIPAVIYFIPYKITFVSAIIPTFWSFRSLELANGSLPKFGIHLIVGTL
                     WYLIVFYLLHR"
     gene            complement(685..1374)
                     /locus_tag="VGA21_11575"
     CDS             complement(685..1374)
                     /locus_tag="VGA21_11575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651193.1"
                     /translation="MNTLVPFLRLDSTLLFRNKLFHISAIITIIYIGLFYLLKPLGNT
                     HIPLIVLIYNDPVIMGYMFAGVLWLFDKNQNTIQAISVLPISKSNYIISKVITLSILA
                     VILSFIMALSAVGIRFNPIHLTISVFLSSVMFCCLGFIIGSISSNFLAFLMKSLPVFI
                     LFATPVTVLFDGVNPVYFILVPSLGSILVMQSAFVAVEVISILLGYIHMCFWTAISIF
                     VCYRITIPRLL"
     gene            complement(1371..2222)
                     /locus_tag="VGA21_11580"
     CDS             complement(1371..2222)
                     /locus_tag="VGA21_11580"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9651194.1"
                     /translation="MLTVGNLSFRYPNNQEDTLKGISFSIQKGKIFGFLGPSGSGKST
                     TQKIVYKLLKGYTGTIIFEDKPLPDWGLEFYEKIGVCFELPNHYTKLTARENLNFFSS
                     FYPIKRKNSDYWLDRVGLIADADKLVSSFSKGMKVRLNFVRAILHDPEVLFLDEPTTG
                     LDPINAGKVKQIIKEMRDSGKTIFLTTHNMFDADQLCDEVALLCNGKIQALDSPSKLK
                     LQHGEKKVIVTQSGDPGNPLTFPLDQLGEIQQFQSILKSNTIETIHSKEATLEEVFIK
                     LTGQQLS"
     gene            complement(2231..2626)
                     /locus_tag="VGA21_11585"
     CDS             complement(2231..2626)
                     /locus_tag="VGA21_11585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651195.1"
                     /translation="MEPQLIRFIYNDELYRLEEKKSGIYRMLVAVDDNYDHQKELLDK
                     ILASVNLSPDDVEISKISGDIKISDLNFSTLISFGIKIREKPNLKPNSLITIDDKTII
                     TAESLDTIANDSTNEHKKKLWSLLKNHFK"
     gene            complement(2629..3150)
                     /locus_tag="VGA21_11590"
     CDS             complement(2629..3150)
                     /locus_tag="VGA21_11590"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014018707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEX9651196.1"
                     /translation="MENVVKNPRHSLATMTELILPNDTNTLNNLMGGKMMHWMDVVSA
                     ICAQRHSNSIVVTASVDNVSFQNPIQLGCVVTLKAFVTRSFSSSMEVYIEVWAENIPD
                     GRKWKSNSAFFTFVAVDKNGKPKRVPELVPETDEEKELYAGALRRRQLRLVLAKRMKP
                     EDAKELKSIFKLD"
     gene            complement(3155..3814)
                     /locus_tag="VGA21_11595"
     CDS             complement(3155..3814)
                     /locus_tag="VGA21_11595"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010608589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HEX9651197.1"
                     /translation="MSMLVDSYKHRGLRKSLIKTLRSKGIEDERLLDAFNAVPRHYFF
                     DDAFLEHAYQDKAFPIGEGQTISQPYTVAIQTALLEIKEGEKVLEIGTGSGYQACILM
                     ELGANVFSVEYHKKLYEKAKTFLPMMGYRPHLKNADGTKGWPTMAPFDKILVTAGAPS
                     VPQALIDQLKPCGILVIPVGSKNLQKMIRVVKTPTGKIEKKEFNNFSFVPLLGEKGWK
                     K"
     gene            3920..4513
                     /locus_tag="VGA21_11600"
     CDS             3920..4513
                     /locus_tag="VGA21_11600"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018622423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEX9651198.1"
                     /translation="MFTGIIEATGKIKSIETSGTNLVFEVESKISKELKVDQSISHDG
                     VCLTVTKKGRGVHYVTAVAETLKRSTLKHLKPGSMVNLERCLPASGRFDGHIVQGHID
                     QTARCNVIEDANGSWLMEFKYDENLGNIAVDKGSICINGISLTCFDTKPGFFRVTIIP
                     YTMENTNLGILKAGDHVNLEFDILGKYVQRIMKEKEA"
     gene            complement(4491..5078)
                     /locus_tag="VGA21_11605"
     CDS             complement(4491..5078)
                     /locus_tag="VGA21_11605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013453626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX9651199.1"
                     /translation="MLEFLENIDQKLFLLINGWHNPAVDSFMYYITDTEFWFPFYGLL
                     LIFLVWKLKWKAIPVVAFVLITVGLSDLITSQFMKPFFQRLRPCHEPELMNLVHIVDG
                     CGKKYGFASGHASNSFALATFLWVSLRHTFKPAFWIFGWSILVSYSRIYVGEHYPGDV
                     IAGALVGSISAIILYYLFMKINPYLPGKIKLPSPS"
     gene            5173..7284
                     /locus_tag="VGA21_11610"
     CDS             5173..7284
                     /locus_tag="VGA21_11610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009580709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX9651200.1"
                     /translation="MAKASSRKVMSSNRIAEKKKTDVKILKKAYSLMCTSKRMAEIYD
                     ENKEICSKYVHSTSRGHEGVQLAAAFHLKPYDYACLYYRDESILLGIGLEPYELMLQL
                     MGKRDDPFSGGRIYYGHPSLKREGFPIIPHQSSATGMQAIPATGMAQGVAYLESQKLR
                     KFPKPPVVLCSIGDGAMTEGEVSEALQMAVLKKLPIIYLVQDNEWGISATGKEMRSMD
                     AFEFALGFKGLARMKADGSDFQNCYEGIGKAFDFVRRERGPILVHAKCPLLGHHTSGV
                     RKEWYRKEEDLKIHSLSDPLPKLKKLLIDSGVGKSELSSLKSEAEAKVKKDFEKSLAS
                     LDPVPEEFDTHEFAPTLVTVEKGNRRPKDAEKVVMVDAALHAIDEIMKTFPESLFYGQ
                     DVGGTLGGVFREAATLAQKYGDERVFNTPIQEAYIVGSTAGMSATGCKPIVEIQFADY
                     IWPGMNQLVEELSKSCYLTKGKFPVQSLIRIPIGAYGGGGPYHSGSIESTLLNIRGIK
                     VIYPSNAADMKGLLKAAFLDPNPVVSLEHKGLYWSKVPGTGDAKTFEPDADYIIPLGK
                     GKIVVSASEENIRAGKACLVITYGMGTHWAWNASKEFPEIVTILDLRTLNPLDWELVE
                     EQVNRHHRAMVLTEEPILNSFAESLAGRISGRCFENLDAPVMTLGAANIPAVPLNVDL
                     EKMMLPSAEKTAKIIRQLLEY"
     gene            complement(7286..9754)
                     /locus_tag="VGA21_11615"
     CDS             complement(7286..9754)
                     /locus_tag="VGA21_11615"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HEX9651201.1"
                     /translation="MRDKARINFSFSLGAGFSFILCSKILTLLLLLNSCIGYRYLKDD
                     QKILYNQKIEGNRKFNKEELKQYFTQKRNRRFLLLPFTPYVYIYKWGENGFSTERLNN
                     KKSRIEVKFDKKIATKEDRRGKVSRLKARKASRLSKVDRILQEGNTGMRVGEPLAVYD
                     SISTKNSATRLQLFYRSNGYFDALVHHEEDTRKKRVNVTYKVAEGKPYFIDSVLFVTK
                     DDSIMHLFQETSRESRINKGDIFTQENLSNERERITALLRNKGYFDFSRQYILFQVDS
                     SFGNHTLWVRTEILVPENRNAHKVFTLDSVNFVMDAQVNIPAGQRTQSPFEGITYLYF
                     EKPYSRKIPNRRIFIRKGELYSHENTLNTQRQLLNMNNFKFININYDTTGGKFVANIF
                     ATLLDEFQISNEVGINYSDAQGFPGPFYNLSLLRRNAFRTLENFETSFRIGLEGVASA
                     TSNEIYRSIEAGGTLAFTFPQFILPIGRNNRLKLGRFNPKTRLQTGYTYNDRPEYRRS
                     NFNLSNTYTWENKRNAQYRMTIGDLSIINSELDSAFEAYLIQLQQRGNNLINTFDPSI
                     VGSIIFSPTFNINQYGSYTRNSTWLRLWIENGGLIMYEINKRYLETFGLEGYKFVKAS
                     LDYRKHRPRENNSSISFRFSTGFAYPYSNNKILPYEKYFFAGGSNSVRGWRPRRLGPG
                     SYTPYDSTDGKITYRFEQQGEVLIESSLEFRQKIAGILAGALFLDAGNIWTIRDDPNR
                     PGSQFKINKFYKEIALAGGAGIRFDFSFLVIRFDLGYKLYDPARPVGRRFFLDPNFNS
                     PPFDRSLNREIEPWILNIGIGFPF"
     gene            9765..10448
                     /locus_tag="VGA21_11620"
     CDS             9765..10448
                     /locus_tag="VGA21_11620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013929729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEX9651202.1"
                     /translation="MAHKLFLIEGAKSVKELLNSQFDISFLLSTQTFLDQNAALLNKC
                     PFPLFMAKVSDIERSGSLKTNNSAIAVAKIPENKPLAPNKSEYILALDDVRDPGNLGA
                     IIRIADWYGITKLILSTNCADPYNPKTIIASMGSFTRVSFHQTNLFKYLEKSACQKIG
                     ATMEGQSIHKIKIATPAIIVLGNESNGLSKLVLDSVNKTVSIPRFGHAESLNVAMAAA
                     VICDNLKRS"
     gene            complement(10445..>11309)
                     /locus_tag="VGA21_11625"
     CDS             complement(10445..>11309)
                     /locus_tag="VGA21_11625"
                     /inference="COORDINATES: protein motif:HMM:NF028437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEX9651203.1"
                     /translation="FLGKFLTALQKPKVPDSIGLEGNDREFARFAHYKTHYFDNFDLA
                     NESLLRSPVYHSVITEYLDKITMQHPDSLISSADVILSQAMKTEATFRYCLVTLSNKF
                     ESSNIMGFDKVFVHIIEEYYLTGKASWTDDDLLDRLKNRVNQIKPGLIGNQAPSLSLV
                     DTEMNPLDLEHIQAKFIVLYFYDHDCGHCKKKTPLLLSQYSSLKEIDVEVIAVDISTD
                     FEKWKGYVSSNNLTFLNGADPYVRSNFRYEYDIRTTPTVYILDENKTIVGKKIDVEKI
                     PDFINYLSRNN"
BASE COUNT         3476 a         2166 c         2362 g         3305 t
ORIGIN      
        1 ttctgtgcaa caaatagaaa actatcaggt accataaggt tcctactatg aggtgaattc
       61 caaactttgg aagagatcca ttagccagtt caagacttcg aaagctccaa aaagtcggta
      121 tgattgcaga gacaaaagta attttgtagg gaatgaaata gattaccgca ggaataagta
      181 cgaggatatt tataactttg atttgtgcga gtccttcaat cttgtttctg gcaaaggccc
      241 caatgtaaag ggcaatcaat ggagcggtaa aactgaaatg aagggcaaca ataatttctt
      301 tccatatttc gaaatgctca attccacccc agtgtattaa agtaaaattt accaatgttg
      361 atatcaatat gccaaccagg agtcgggaag taaccaggtt gaaccctgaa atgggcagta
      421 cctgaattgc ctgccaaata ttttcttctt tttcttctaa aaatataaaa ccataaataa
      481 aaccaaacaa ggtagctgat tgcagacagg cccacatcaa aataacctgc gaataaggtt
      541 caattggagg gtaaaggttc aaaattgcag gataccccca gcggattatc gcaaacgaaa
      601 agaaggggaa gaaaatcagc aagcgaaata tgggttctcg gaaagtggag cacaaatcag
      661 agtaactcaa tgtaagtaaa gttctcatag cagccttggt atggttatac gataacaaac
      721 aaaaatgcta attgctgtcc aaaaacacat atgtatatac ccaagcagaa ttgagattac
      781 ttccaccgca acaaaagctg attgcatcac taaaatgctt ccaagtgatg ggacgagtat
      841 aaaatacaca ggatttaccc catcgaagag aacagttacg ggagtggcaa acaaaataaa
      901 aaccggcagc gatttcatta agaaagcaag aaaatttgaa gaaatgcttc ctataataaa
      961 gccaaggcag caaaacataa cagatgataa aaatacgctg atagttaaat gtattggatt
     1021 aaagcgaatt cccactgcgg acagcgccat tatgaatgat aatattactg ccagaatgga
     1081 aagagttatt actttggaaa ttatataatt tgattttgaa atgggaagca ctgaaatggc
     1141 ctggatggta ttctgattct tatcaaaaag ccaaagtaca ccggcaaaca tataccccat
     1201 aattaccgga tcattgtaaa taagaactat caatggtatg tgggtattcc caagaggttt
     1261 tagcaggtaa aacaacccta tatagataat tgtaataatg gcagaaatgt gaaacaattt
     1321 attccggaaa agaagtgtgc tatccaacct gagaaaaggc actaatgtgt tcatgataat
     1381 tgttggccgg taagtttaat aaaaacctct tccaatgttg cttctttgga atggattgtt
     1441 tcgatagtgt tgcttttaag tatggattga aactgttgaa tttctccaag ttggtccaga
     1501 ggaaaggtta aagggtttcc aggatcaccg gattgagtca caattacttt tttttcacca
     1561 tgttgcagtt taagctttga tggggagtca agagcctgaa tcttaccatt gcataacaaa
     1621 gcgacttcgt cacacaattg gtcagcatca aacatattgt gtgtagtgag gaagattgtt
     1681 tttccgctgt ctctcatctc ctttattatt tgtttcactt tgcccgcatt aatgggatca
     1741 agcccggtgg tcggctcatc aagaaaaagt acttcaggat catgcaatat ggcccggaca
     1801 aaattgagcc ggactttcat ccccttggaa aagcttgaaa ctaatttatc agcatccgct
     1861 ataagcccta cacggtcaag ccagtaatca gaattttttc ttttgatggg ataaaaggaa
     1921 gaaaagaaat ttaaattttc ccttgcagtc agcttagtat agtgattggg taactcaaag
     1981 caaactccaa ttttttcata aaattccaat ccccaatcag gaaggggctt gtcttcaaaa
     2041 ataatggtac cggtataccc cttgagaagt ttatagacaa tcttctgagt agtggattta
     2101 ccgcttccac tggggccaag gaatccaaaa attttacctt tttgaattga gaaactaatg
     2161 cctttgaggg tgtcttcctg attattggga tatctaaaag ataaatttcc aacagttagc
     2221 attcctgaag ttatttaaag tgattcttaa gaagactcca taatttcttc ttgtgttcat
     2281 tagtagaatc attggcaata gtatcgaggg attcggcggt aataattgtc ttatcatcaa
     2341 tcgtaatgag ggaattcggt tttagattcg gtttttcccg gatttttata ccaaaggaaa
     2401 tcagggtgct gaaattcaaa tcagaaattt ttatatctcc gcttattttg gaaatttcca
     2461 catcatcagg actaagattc actgaggcaa ggattttgtc cagtaattct ttctgatggt
     2521 cataattgtc atcaacagca accaacatcc tgtaaatacc ggattttttt tcttccagcc
     2581 tataaagttc gtcattataa ataaacctga tgagttgggg ttccattgct aatccaattt
     2641 aaaaatagat ttcagttcct tcgcatcttc gggtttcatt cttttagcca acactaaacg
     2701 caactggcgt cttctcaacg caccggcata taattctttt tcttcatccg tctcaggtac
     2761 aagttccgga actctttttg gttttccatt cttatccact gccacaaatg taaagaaggc
     2821 gctgttgctc ttccatttcc tcccatccgg gatattttct gcccatactt caatatatac
     2881 ttccatcgat gaggaaaatg atcgtgtgac aaaggctttg agcgtaacca cacagcccaa
     2941 ctgaattggg ttctgaaatg aaacattatc aactgatgcg gtaactacta tgctgtttga
     3001 atgacgctga gcgcatatcg cggagacaac gtccatccaa tgcatcatct tacctcccat
     3061 aaggttattt aaagtgttgg tgtcgtttgg cagaatcagt tctgtcattg tggccagtga
     3121 atgccgggga tttttgacta cattttccat atctttattt cttccagccc ttttcgccca
     3181 aaagaggaac gaaactgaaa ttattaaatt cttttttctc tatttttcca gtgggtgttt
     3241 ttactactct gatcatcttt tgaagatttt tcgatccaac agggataacc agaattccgc
     3301 agggcttcag ctggtcaatc agagcctgtg gaacggaggg tgccccggcg gtgaccagaa
     3361 ttttatcaaa aggtgccatg gttggccacc cttttgtacc atcggcattc ttcaaatggg
     3421 ggcggtaccc catcattgga agaaaggttt tggctttctc atataacttc ttgtggtatt
     3481 caacggaaaa tacatttgca cctagctcca tgagaataca agcttgatat cctgatcccg
     3541 ttccaatttc caaaaccttt tcaccttcct taatttctaa taaagctgtt tgaatagcaa
     3601 ccgtgtaagg ttgtgaaatt gtctggcctt cacctatagg gaacgcttta tcctggtacg
     3661 catgttccag gaaagcatca tcaaagaaat aatggcgagg caccgcgttg aaagcatcca
     3721 gcagcctttc atcttcaatc cctttagatc ggagagtttt tatcaaagac ttccttaacc
     3781 cccgatgttt atagctgtca accaacatac tcatgcgaat ttaggattaa attttgtaag
     3841 ggaagtacct ttttcttgtc ataacataaa ttgttggggg tctcaaatta gattaaattt
     3901 gattttcaaa acccgcgaaa tgtttacagg cataattgaa gcaacaggaa aaataaaatc
     3961 tatcgaaacc tcaggaacga acctggtatt cgaagtggag agtaaaattt caaaagagct
     4021 gaaggttgat cagagcattt cccacgatgg cgtttgctta acagttacta agaagggaag
     4081 gggagttcat tatgttactg cagttgcaga aactttaaaa aggtctacgt tgaaacattt
     4141 gaaaccggga tcaatggtca acctggagcg atgtttaccg gcgagtggaa gatttgacgg
     4201 acatattgtt caaggccata tagatcagac tgccaggtgc aatgtaattg aagatgcaaa
     4261 cggaagctgg ctgatggagt ttaaatatga tgaaaacctg ggaaacattg cggtggataa
     4321 aggttccatt tgcatcaatg gaatttcgct gacatgcttt gatacaaaac cgggattctt
     4381 cagagttact attattccat atactatgga aaatacaaac ctgggcattt taaaagcagg
     4441 ggatcacgtg aacctggagt ttgatatcct ggggaagtat gtacaacgga ttatgaagga
     4501 gaaggaagct taatctttcc gggcagatat gggttgattt tcataaacag gtaatacaaa
     4561 atgattgcag aaatcgaacc taccagagcc ccggcaatca catctccggg ataatgctcg
     4621 cctacataaa ttctcgagta ggatacaagt attgaccagc caaagatcca aaatgcaggc
     4681 ttgaaggtat gcctcaatga aacccaaaga aaggtagcca aagcaaagga attagaagcg
     4741 tgaccggacg caaatccata tttcttacca cacccatcta cgatatgaac aagattcatt
     4801 aattctggtt catggcaggg tcttaaacgc tgaaagaaag gtttcatgaa ttgcgaagta
     4861 attaaatccg acaatcctac agttataagt acaaaggcaa caaccggaat tgctttccat
     4921 ttcagtttcc atactaaaaa aatcagcaac aacccataaa agggaaacca aaattccgtg
     4981 tcggttatat aatacatgaa ggaatcaacc gcaggattat gccatccatt aatgaggaga
     5041 aataacttct gatcaatatt ttcgagaaac tccagcattt cagttttgca aaggtattca
     5101 attaatagga aatcagactc aaggctatat tagagattaa ttacaacgaa ttttggtttc
     5161 aaaatcctta aattggcaaa agcttcatcc agaaaagtta tgtcatcgaa ccggattgcc
     5221 gaaaagaaaa agacagatgt taagatattg aaaaaggcgt attctttaat gtgtacctct
     5281 aaaaggatgg cagaaatata tgatgagaac aaggaaatat gctctaagta tgtacacagc
     5341 acctcacggg ggcatgaggg agttcaattg gcggcggcct ttcacttaaa gccgtatgat
     5401 tatgcctgtc tttattatcg tgatgaatcc attttgttgg gaattggcct ggagccctat
     5461 gaacttatgt tacagttgat ggggaaaagg gatgatccct tttcgggagg aagaatttat
     5521 tacgggcacc cttcgctgaa gcgtgaagga tttcccataa ttccgcacca gagctcagcc
     5581 accggcatgc aggcgatacc ggcaactgga atggctcagg gtgttgctta ccttgaatct
     5641 caaaaactca ggaagtttcc aaagccgccg gtagtcctat gttctattgg agatggagcg
     5701 atgacagaag gcgaggtttc ggaagctttg caaatggccg tgcttaaaaa acttccgatt
     5761 atttacctgg tgcaggacaa tgaatggggt atttctgcta ctggcaaaga aatgcgttcc
     5821 atggatgctt ttgaatttgc tcttggtttt aaaggtttgg caagaatgaa agcagatggt
     5881 tctgattttc agaattgtta tgaaggcatt gggaaagcgt ttgactttgt aagaagggaa
     5941 cgcggaccca ttctggtaca tgccaaatgt ccattgcttg gtcatcatac ttcaggtgta
     6001 agaaaggaat ggtatagaaa ggaggaagat ttgaagattc attccttgag tgatccattg
     6061 ccaaagctca agaaattatt aatcgactcg ggtgtcggga aatctgaatt gagttccctg
     6121 aaatctgagg ccgaagcaaa ggttaaaaag gattttgaaa aatcactggc atcacttgat
     6181 ccggttcctg aggaatttga tacgcacgaa ttcgctccta ctttggttac agtggaaaaa
     6241 ggaaatagaa ggccaaaaga cgcggaaaaa gttgtaatgg tggatgctgc tttgcatgct
     6301 attgatgaga taatgaaaac atttccggag tcattatttt atggtcagga tgtcggaggt
     6361 acccttggcg gtgttttccg ggaggccgca accctggctc agaagtatgg agatgaaaga
     6421 gtttttaata ccccgattca agaagcatat attgtggggt caaccgctgg aatgtctgct
     6481 accggttgca agccaatagt agaaatacaa tttgctgatt atatctggcc cgggatgaat
     6541 caattggttg aagaattaag caagagttgc tatctgacta agggaaagtt tccggttcaa
     6601 tcccttattc gaatccccat cggggcttat ggtggcggcg gtccctacca cagtggaagt
     6661 attgaatcca ccttgctgaa catccgcggg attaaagtga tttatccatc taatgcagct
     6721 gacatgaagg gtctgctgaa ggctgcattt ctggatccaa atcctgttgt atccctggag
     6781 cacaaaggat tgtactggtc aaaagttccg ggtacaggag atgccaaaac ctttgaaccg
     6841 gatgctgatt acattattcc actaggtaaa ggcaagatcg ttgtttctgc ttcagaggaa
     6901 aatataagag cagggaaggc ttgcctggtc ataacatatg gaatgggaac ccactgggca
     6961 tggaatgcga gcaaggagtt tccggaaatt gtaaccatcc tggatttgag aacgctgaac
     7021 ccattggatt gggaacttgt cgaagaacag gtgaaccgac accacagggc gatggtgttg
     7081 acggaggagc ccattttaaa tagctttgct gaaagtttgg ccggcaggat ttccgggaga
     7141 tgttttgaaa atctggatgc gccggtaatg actctgggag cggccaacat tccggcggtg
     7201 ccgttaaatg ttgatctcga aaagatgatg ttaccaagcg cggaaaagac tgcaaaaata
     7261 atcaggcaat tgctggaata ttaacttaga atggaaatcc aattccaata tttagtatcc
     7321 agggttctat ctctcgattt agacttctgt cgaatggtgg tgaattaaag ttagggtcca
     7381 ggaaaaatct tctacccact ggtcgtgccg gatcataaag tttgtaaccc aaatcaaatc
     7441 ggatcaccag gaaggagaaa tcaaaccgga ttccggcccc tccagccagc gcaatctctt
     7501 tgtaaaattt atttattttg aattgacttc cgggccggtt aggatcgtcg cgaatggtcc
     7561 agatattacc ggcatcaaga aacagggcac ctgccagaat accggcaatt ttttgcctga
     7621 actcaagact ggattcgatc aaaacttcac cctgctgttc aaaccggtag gtaattttac
     7681 catccgtgga gtcataagga gtataagaac ccggtcccag acgacggggc ctccagcctc
     7741 gcacactgtt gctgcctcct gcgaaaaagt atttttcata ggggagaatc ttattattgg
     7801 aatatggata tgcgaacccg gtggagaacc gaaatgaaat actagaattg ttttcccttg
     7861 gtctgtgttt cctgtagtca agactagctt tcacgaattt gtaaccttcc agtccgaatg
     7921 tctccaaata tctcttatta atctcataca ttattaaacc tccgttttca atccacaatc
     7981 taagccaggt tgagttccgg gtataactac cgtactggtt aatattgaag gttggtgaaa
     8041 atattatact ccccactatg gaaggatcga aggtattgat aagattatta ccgcgttgtt
     8101 gcaattgaat caggtatgct tcaaaggctg aatctaattc agaatttatt atgctcaagt
     8161 caccaatggt catccggtat tgcgcatttc tcttgttttc ccaggtgtaa gtgttggata
     8221 aattaaaatt agatcttcta tattcaggcc tgtcattata ggtatacccg gtctgaagtc
     8281 ttgtttttgg attgaacctt cccagtttga gccggttatt tctgccgatg ggtaaaataa
     8341 actgaggaaa ggtaaaggcg agggtaccac ctgcttcgat acttctgtaa atttcattac
     8401 ttgttgccga tgcaacacct tccaatccga ttctaaaact tgtttcaaaa ttttcaagag
     8461 tacgaaacgc atttcttcta aggagagaaa gattgtaaaa aggaccagga aatccctggg
     8521 catcactgta attgatacct acctcattcg aaatttgaaa ttcatcaagg agtgtagcaa
     8581 atatgttggc cacgaatttt ccacctgtag tatcatagtt gatgtttatg aatttgaaat
     8641 tattcatatt aagcaattgt ctttgagtat tcaaagtatt ttcgtggctg tacaactcac
     8701 ccttccttat aaagattctc ctatttggaa ttttgcggga ataaggcttt tcaaaatata
     8761 aataagtaat tccttcaaac ggactttgtg tgcgctgacc ggcaggaatg ttgacctgag
     8821 catccatcac aaaattgaca gaatccaaag taaagacttt gtgagcgttc ctgttttcgg
     8881 gtaccaatat ttcagttctg acccaaagag tatgatttcc aaatgaggaa tctacctgga
     8941 agagaatata ttgcctgcta aaatcgaagt aacctttgtt tcttaataat gctgtgattc
     9001 tctccctttc attactaaga ttttcctgcg taaaaatgtc tcctttatta atccggcttt
     9061 cccgcgaagt ttcttgaaaa agatgcatga tagaatcatc tttagttacg aataatacgc
     9121 tgtctataaa atacggtttg ccttcggcta ctttataagt aacattaact ctctttttgc
     9181 gcgtatcttc ttcatgatga accagagcgt caaagtatcc gttgcttcgg taaaagagtt
     9241 gaaggcgagt tgcactattt ttagtagaga tactatcata aaccgcaagg ggttctccaa
     9301 cgcgcatgcc cgtattgcct tcctgtaata ttcgatctac tttgcttaac cgtgaggctt
     9361 tcctggcttt taaccttgat acttttcccc gccgatcctc ttttgtagca atcttcttgt
     9421 caaacttaac ctcaattctg gacttcttat tattcaaccg ttctgttgag aatccatttt
     9481 caccccattt gtagatataa acgtatggtg taaaaggaag taaaagaaac ctgcgatttc
     9541 tcttctgggt aaaatattgc tttaattcct ctttgttgaa cttccggttg ccttcgattt
     9601 tttggttgta caggattttc tgatcatctt tgagatagcg gtatccgata caactgttta
     9661 gaagcagaag aagggtaagt atttttgaac aaagtatgaa gctaaaaccg gcacctaaag
     9721 aaaagctgaa attaattcgc gctttatctc tcaaaaagaa tcgggtggcg cataagcttt
     9781 tcttaattga aggagccaaa agcgttaagg agcttttgaa ttcgcaattt gatatttctt
     9841 tcttgctatc cactcaaact tttcttgatc aaaacgctgc tttactcaac aaatgcccgt
     9901 ttccgctttt tatggcaaaa gtaagtgata ttgagagatc gggttcttta aaaaccaata
     9961 attctgccat agcggtggca aaaataccgg agaacaaacc attggctcct aataaatcgg
    10021 aatatatttt agccctggat gatgtaagag atccgggaaa tctcggagcg ataatccgaa
    10081 tagcggactg gtatggaata actaagctta tattatcaac gaactgtgct gatccctata
    10141 atcccaaaac aatcatagcc agtatgggtt catttacccg ggtaagcttt catcaaacaa
    10201 atctttttaa atatctggag aaaagtgcct gccaaaaaat tggagcaacc atggaaggtc
    10261 aaagtattca taagataaaa atagcaacgc cggcaattat tgtactaggg aatgaatcta
    10321 atggcttgag taagcttgtg cttgattctg taaacaagac agtcagcatt ccaagatttg
    10381 gccatgcgga atcattaaat gttgcgatgg cagctgccgt aatttgtgat aatttaaaaa
    10441 gaagttaatt gttcctgctc aggtagttaa taaaatccgg aattttttcc acatcaatct
    10501 tctttccaac aatggtttta ttctcatcga gtatatagac tgtaggagtt gtacgaatat
    10561 catattcata cctgaaatta gatctgacat aagggtcggc tccattcagg aatgttaaat
    10621 tattactgct cacataaccc ttccattttt caaaatcagt acttatatca accgcaatta
    10681 cttcaacatc tatctccttt aatgacgaat attgtgaaag aagcaggggt gtctttttct
    10741 tgcaatgccc gcaatcatga tcgtagaaat ataacacaat aaatttcgcc tggatatgtt
    10801 ctaaatcgag tgggttcatc tcggtatcaa ccagcgatag ggaaggtgcc tgattgccaa
    10861 tcagacccgg ctttatctgg ttaaccctgt ttttcaatcg gtccagtaaa tcatcatcag
    10921 tccaggaagc ttttccggtg agataatatt cttcaataat gtgaacaaag accttatcaa
    10981 aacccatgat atttgaactt tcaaatttgt tggaaagggt tacgaggcaa tatcgaaacg
    11041 tggcctcagt tttcattgcc tgactcagaa tcacatcagc ggatgagata agactatccg
    11101 gatgctgcat tgttatctta tccaaatatt cggtaatcac agaatgataa acaggggagc
    11161 gaaggagact ttcatttgca agatcaaaat tgtcgaagta atgtgtttta taatgagcga
    11221 atctggcaaa ttcgcgatcg ttaccttcaa gaccaattga atcgggcact ttgggttttt
    11281 gtaatgctgt aaggaatttg cccaggaaa
//
LOCUS       DASWHX010000178         2616 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_574839, whole genome shotgun sequence.
ACCESSION   DASWHX010000178 DASWHX010000000
VERSION     DASWHX010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 2616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2616
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_574839"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(178..1431)
                     /locus_tag="VGA21_11630"
     CDS             complement(178..1431)
                     /locus_tag="VGA21_11630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018479443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HEX9651204.1"
                     /translation="MEQTSQALITDDTVVIGLLMVILALVFWTHNIQTPFWKKFYTYI
                     PSVLLCYFIPSVFTSLGVIDGEASNLYFVSSRYLLPTCLVLLTLSIDLKEIMKLGTKA
                     VIMFFTGTVGVILGGPIAILIFSVIAPDIVGGVGPEAVWRGMTTVAGSWIGGSANQAA
                     MLEIFGSSPEMFSKMIAVDVIVANIWMAFLLYGAGQSGRLDKLFKADATAIEHVQKRM
                     DDFKASVAKIPTLTDIMIILGVGFGATSFSHWVGAPISEFIEVNFPYLEKLSLTSSFF
                     WLVVIATTIGLILSFTKARNLEGAGASKIGSAMLYVLIASVGMRMNLGAILDDPGLFL
                     VGIVWISIHVALLLLVAKLIKAPFFFTAVGSQANIGGAASAPVVAAAFSPSLAPVGVL
                     LAVLGYAVGTYGAWLCGILMQNVAP"
     gene            1616..2311
                     /locus_tag="VGA21_11635"
     CDS             1616..2311
                     /locus_tag="VGA21_11635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651205.1"
                     /translation="MNKRKPIIQFVIFLIITAIPACSTNTQVNHAGNLRDIMMGTDIS
                     AKMDLNELKGIPHLYAVGALENLGGEILIYDGYPIISHFKNVLYVTDHSFDFNATLIV
                     YSQVEKWQSIEIPESLRTREGLETFISEISGPYGLRGKPFPFLLEGEVSNAYWHIVNG
                     IGGENHSDSGIRGNIKNEYVRIVGFYSKDHQGIFTHHDSNMHAHVINADESIAAHLAD
                     IELGKNMVLKIPD"
     gene            2350..>2616
                     /locus_tag="VGA21_11640"
     CDS             2350..>2616
                     /locus_tag="VGA21_11640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002693572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-protection protein"
                     /protein_id="HEX9651206.1"
                     /translation="MEVRFFSTPDHFRKWLQKNHNKANELWVGYYKKSSGKQSITWPE
                     SVDQALCFGWIDGIRKSIDAKSYKIRFTPRKPTSHWSKINVSKVK"
BASE COUNT          844 a          530 c          544 g          698 t
ORIGIN      
        1 atgcgtttcg gaagtagaat gaaatttcga ccaattgggt ttcaggggat cttcagattg
       61 agcttgaatt ccggttgtga ccagaactga tattcctccc agcaggaaat tcttcaggta
      121 attcatatct aattgttttt ataaccaagg ttaatttaca aaaaccaatc ggaaatatca
      181 tggtgctaca ttttgcatga gtattccgca aagccaggca ccataagtgc caacggcata
      241 ccccaatact gccagcagca ctcctaccgg agctagtgag ggactgaaag cggcagcaac
      301 tacaggagcc gaagcggctc cgccaatatt agcctgactt cctaccgctg taaaaaagaa
      361 cggagctttg ataagttttg ctaccagcaa aagcagcgca acatgaatgc taatccagac
      421 aatacccaca aggaaaagtc ccggatcatc caggatggct ccaagattca tgcgcattcc
      481 gaccgaagca atcaaaacat aaagcatagc gcttcctatc ttgcttgccc cggctccttc
      541 cagatttctc gcttttgtaa aggaaagtat caaaccaatg gtagttgcaa ttaccaccag
      601 ccagaaaaaa gaagaagtga gacttagctt ttcaaggtag ggaaaattca cttcaataaa
      661 ttctgaaata ggggcaccaa cccaatgaga aaaggaagtt gcaccgaaac caacacccag
      721 aataatcatg atatctgtaa gagtgggtat ttttgctact gatgccttga agtcatccat
      781 tcttttctga acatgttcaa tagcagtggc atccgcttta aacaatttat caagccttcc
      841 ggattggccc gctccgtaga gcaggaatgc catccagata tttgcaacaa ttacatccac
      901 ggcgatcatt ttggaaaaca tttcagggct tgacccgaaa atttccagca tagcggcttg
      961 attcgcactg ccaccaatcc aacttcctgc aacggtggtc atccctcgcc atacagcttc
     1021 tggtcctacc ccaccaacaa tgtcaggggc aatcaccgaa aagattaaaa tggcaatggg
     1081 gccacccagg attacaccaa ccgtacccgt aaaaaacatg atgaccgctt ttgtacccag
     1141 tttcattatt tctttcaggt caatgcttag tgtaagaagt accaaacaag taggaagcag
     1201 atatcttgat gagacaaaat acaagttaga tgcctcgcca tcgatcacgc ctaatgaggt
     1261 gaagacggaa gggataaaat agcataaaag gacagagggg atataggtat aaaatttctt
     1321 ccagaaagga gtttggatgt tatgcgtcca gaataccaaa gccagaatta ccatcaatag
     1381 tccaatgacg acagtatcat cggttatcag agcttgtgag gtttgttcca tttaaattta
     1441 gtattttctg ccccaataaa atcatttcat ggctaacgac caaaatgtat tgaaaattaa
     1501 actcttaaaa atatcctggt gtatttctga ataattttaa cctatttaga atcagggagt
     1561 cgctagccgt aatttattgc cttatattta tgaaaaaatc agcacgaaaa taggtatgaa
     1621 caagagaaag ccaattattc aatttgtaat atttctaatt atcacagcga tacccgcctg
     1681 tagtacaaat acacaggtga atcatgccgg aaatttgagg gatattatga tgggaacgga
     1741 catctccgca aaaatggacc ttaatgagtt gaaagggatc ccccatctat acgctgtcgg
     1801 tgctcttgaa aacctgggcg gtgaaatttt aatctatgat ggttatccga ttatctccca
     1861 ctttaagaat gtattatatg taactgacca tagttttgat tttaatgcaa ctctcattgt
     1921 ttattcacag gtggagaagt ggcagagtat agaaattccg gaatccttgc gaacaaggga
     1981 aggcctggag acttttattt cagaaatctc aggtccttac gggctgcgtg ggaaaccatt
     2041 tccatttctg cttgaaggag aagtctccaa tgcttactgg catatcgtaa atggaattgg
     2101 cggcgaaaat cattcagata gcggtatcag gggaaatata aaaaatgagt atgtaaggat
     2161 tgttggtttt tattctaaag atcatcaggg tatatttacg catcatgatt ccaacatgca
     2221 tgctcatgta attaacgctg atgaatcaat agcagcacac ctcgctgata ttgaattggg
     2281 gaaaaatatg gttttgaaaa tcccggatta aattttgatg attatagtta ctttttaact
     2341 ttaaaatgca tggaagtaag attcttctct actccggacc atttccggaa atggcttcaa
     2401 aagaatcata acaaagcgaa tgaactttgg gtgggttatt acaagaaaag ttcgggaaag
     2461 caaagtatta cctggccgga atccgtggat caggcacttt gttttgggtg gattgatggg
     2521 attaggaaat caattgacgc gaagagttat aaaatccggt ttactcccag aaaaccaacc
     2581 agccactgga gcaagataaa cgtgagcaag gtaaag
//
LOCUS       DASWHX010000179        24364 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_575499, whole genome shotgun sequence.
ACCESSION   DASWHX010000179 DASWHX010000000
VERSION     DASWHX010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 24364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24364
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_575499"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..292
                     /locus_tag="VGA21_11645"
     CDS             <1..292
                     /locus_tag="VGA21_11645"
                     /inference="COORDINATES: protein motif:HMM:NF024869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HEX9651207.1"
                     /translation="LENLYPDKNTFAVILSHDPKIDDAALEILLKKEIPYIGALGSKK
                     THEKRKTRLLEKGFTEAEIARIHGPVGININAQTAGEIALSIMAEIIEVKNS"
     gene            596..1510
                     /locus_tag="VGA21_11650"
     CDS             596..1510
                     /locus_tag="VGA21_11650"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX9651208.1"
                     /translation="MNTKNPLFKAYLALAIVCFFWGTTYLAIRIGVQFVPGFMMAGIR
                     NIIAGLITCSYFVFIGTKMPDRKLWGIIFVRALLLIVLGNAMVHWAEESVESGLAAIL
                     AATVPLWVAGISSIGFRSVTLNKKSLAGLLLGFAGVLCIFITGKEDLFHLSSIWGVLA
                     MMIAAIGWSLGTVYSSVNKINLPVIFTAGIQMLLGGIISIMISFSIGEQVSVLAIQWQ
                     GWTAIFYLVAFGSILSYNSFIYALDKLPATLVGIYAYLNPLVAVFLGWLILDERMDAW
                     VAFGILITLYGVYLVNKGFSLRAITPDK"
     gene            1583..3973
                     /locus_tag="VGA21_11655"
     CDS             1583..3973
                     /locus_tag="VGA21_11655"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9651209.1"
                     /translation="MLKNYLLITLRNIRKQKLYSFINLTGLATGIAACLIIFLFIRDE
                     KSFDAFHKKNGVIYRLCEVQSFPGTLTQNVALSMPAMGPHMWTEFPEITSFTRYFGGT
                     DWIFNIGEKAIKVPQLARVDSTFLQIFDFELITGDKTTALNDPYSILITEETAIKFFG
                     TTEVLGRQVARGEDNYQITGILKNVPENSHLQFDALISMSSITSQSPEFNDRWGSNYL
                     VTYFIIPEGTNLEAMAERFPEFLVSKMGDEITELYQLYLQPLSQVHLGSIDTQHDYRN
                     YRKFDGKYISIFMVLAGFVLLIAGINFMNLSIYRSVSRSKEVGLRKSLGAQKTQLVAQ
                     FIGESVIFSCLALVTSLAITALLIPSLNLMTGRTMDLASILTEPFPVFALLGITFLVG
                     LLAGVYPAFYVSTFNPATVLKGGSSSSKQSVFKSGLVVFQFALAIGMIAGTFITLNQL
                     RYMKNKDLGFDKSRIVLIPASQESDNKYRTIYNEFKNLPGVDGVTATGQRLGNNLHQW
                     SARAKNEEELLDITISQVNVDKDFLEVYDIELLDGRTFTDQIHSDTRGQSFLINEKLA
                     KELGWENPVGTQLGFSGVDSIGTVIGVVKNFNYNSLHYDINTLGIYMRPDWGYDEISV
                     KIKAGAGEEVFTGLKKIWDQNVSDRPFEYSYLDEHFLAIYRADTQVTKVVASIATLAI
                     LIAMLGLFGLSALSTLNRVKEVGIRKVLGASGNSLLMLLTNKFTLLILSSFIIAAPVT
                     YFFMKGWLENFAFRIEITFAPFLVAALISFLVAFITVGYHAIKALSVNPTDILRYE"
     gene            complement(4011..4538)
                     /locus_tag="VGA21_11660"
     CDS             complement(4011..4538)
                     /locus_tag="VGA21_11660"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HEX9651210.1"
                     /translation="MEWFKDYKVIVTVFFVVLYVISRFFATRIIKRRARKNQFEEKRT
                     LYLLKLFHSINLLIITVLIVLTWDINLRILRDYLLGFLTIVGVAIFASWSILSNLTSS
                     VILFFYYTYKVGSIIRIVDGTNSITGRILEITLFYIKLQTKDRNIISYPNNLAIQRPI
                     IELKDWDSETGIPIR"
     gene            complement(4600..5148)
                     /locus_tag="VGA21_11665"
     CDS             complement(4600..5148)
                     /locus_tag="VGA21_11665"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX9651211.1"
                     /translation="MVRKDLTDEALVELLQHEGNARFFPLLVTRYEKEIFKRCASYIK
                     EKETAADLTQEILIKVYLQLPRFRKEAKFSTWLYAIIHNTCIDHLRKSANRNTIIIKH
                     LADLTEDIPDLEEQVSPEITTQVLTQLLNQITPEERLILILKYQEKQSIKDIHSSLGI
                     SESAVKMRLKRAKQKVFKLINK"
     gene            5219..5935
                     /locus_tag="VGA21_11670"
     CDS             5219..5935
                     /locus_tag="VGA21_11670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009581260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HEX9651212.1"
                     /translation="MIAKETKAVLELDIQPQPTEETCGPTCLHAVYNYYGEKISLSTV
                     ISEVKQLASGGTLGVLLGTHALEHGFDVTIYTYNLDIYDPTWFHANVSIPDKLREQLK
                     YKKGKKFKFASEAYLKFLEIGGQIKFKELTPALITNFIEEGIPVISGLSVTYLYQSAR
                     EIGEKNLYDDVKGEPAGHFVIVNGYDKETRSVYIADPYKPNPMIDGHFYKVKTHRLTN
                     AILLGIVTYDANLLVIRPKK"
     gene            5940..7382
                     /locus_tag="VGA21_11675"
     CDS             5940..7382
                     /locus_tag="VGA21_11675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009581261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RimK family protein"
                     /protein_id="HEX9651213.1"
                     /translation="MSKLVIAENPKKWTIHQEDVVVITPNEYFSDPLYQTSRYKVINL
                     CRSYQYQTLGYYVSLLGEARKHKIIPKVSTLQEFRFPSIVRDETGGFEELIQNEFKSV
                     KEKEIELNVYFGFTHDAEHNKIGILLFNLYQAPLLKAVFEKQKVWELQSLRPLQLNEI
                     DDNERPTLNQALELYLKGRQVVRKSLSRKKYDLAIMVNPDDANPPSDAKALQKFIKAA
                     GKEGLNAEIITKNDFAKLVQYDALLIRETTAVNNHTFRFAKKAVSEGLVVIDDPDSML
                     RCTNKVFLSELLKTHNIPSPKTVILQDSKLTDELRELKFPFILKEPDSAFSKGVKKVN
                     NETELKETLKDFFQKSDLVIAQEFIPTDYDWRVGVLDDEPIFVCKYFMARNHWQIMDW
                     TKKVRKEGKTETIAVEDAPPKLIDIALRAAKAVGNGLYGVDVKEINNKFYIIEVNDNP
                     NIDSGIEDKVLKLDLYRKIMATIVKRIKAS"
     gene            7390..8652
                     /locus_tag="VGA21_11680"
     CDS             7390..8652
                     /locus_tag="VGA21_11680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009581262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-cysteine ligase family protein"
                     /protein_id="HEX9651214.1"
                     /translation="MPNIQRLHLFQGYGVELEYMIVDKETLNVRPLAADLLTKAIGSL
                     EDDFINGEITWSNELVMHVIELKSTLPVTDMVKFSTHFKNNILMANNILDEFNAMLMP
                     TAAHPWMNPQTDTVLWPYGSKEIYETYNKIFNCQGHGWSNLQSSHLNLPFYDDEEFAR
                     LHAAVRLVLPILPALAASSPVLEGKFSKFYDKRLSYYEKNQMRIPSITGKVIPEKLFS
                     RRSYQKFIFDKIKTDLQKHDTLGIMDPVWVNSRGATARFDRGTIEIRLLDIQECPAAD
                     LAIIALVVHTLKWLVAEEGIKLHEQMEFGVEPLYKIFNATIRDAEETIINDPKFLSIF
                     KIEKSELKAGELWKEIHSIVKGKYPDEMVPWENYLKNIFEQGTLARRIMNIAGNEPTL
                     ETLKLVYKELSDCLQENEMFNSWIEDVL"
     gene            8631..9347
                     /locus_tag="VGA21_11685"
     CDS             8631..9347
                     /locus_tag="VGA21_11685"
                     /inference="COORDINATES: protein motif:HMM:NF016873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HEX9651215.1"
                     /translation="MDRRRIIISCEHAGNKVPTQFRFLFKGKESVLQSHLGWDPGAFS
                     VARSLADALKVRLFHQDYSRLLIECNRSLDNPELFSKYSTGLNDMVKDYLIRKYYLSY
                     RKKVERAIGKILDKGKPVLHLSIHSFTPALNGTKRKADLGLLYDPDRRSEEDFSVHLK
                     NELEILSPNLLVKFNYPYLGTDDGFTTYLRTKFPDDSYAGIEIEVNQNMTAWPARKKI
                     QRILTECIRKMYFEVTHELN"
     gene            9354..9791
                     /locus_tag="VGA21_11690"
     CDS             9354..9791
                     /locus_tag="VGA21_11690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020527502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RimK/LysX family protein"
                     /protein_id="HEX9651216.1"
                     /translation="MDDRKVIGRSDKIDLPEFGIFNLEAKIDTGAAICAIHCHEIQLV
                     KKGNEELLQFQLLDPSHPGYNEKTYYSHHFELARIKNSFGQTEERYVVTTPVIIFGKT
                     IETRFSLTDRKDLKFPILLGRQFLRKRFIVDVAKKNISFKKKK"
     gene            9788..10705
                     /gene="rimK"
                     /locus_tag="VGA21_11695"
     CDS             9788..10705
                     /gene="rimK"
                     /locus_tag="VGA21_11695"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020527503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HEX9651217.1"
                     /translation="MKKLKIAVLSRNPKLYSTQRLVEAITTRGHESMVIDHLKCDLVM
                     DEKGPAIYYKGMKLEKIDAVIPRIGASVTFYGSAVVRQFEMMHVFSAIDSLAITRSRD
                     KLRSLQILSRAGVGMPKTAFTNYSSEKNEILHEIGSPPVVIKLLEGTQGLGVVLAETR
                     KAAKSVIEAFEGLKARIILQEFIEEAGGADVRVFIVNGVVVGAMKRQGKEGEFRSNLH
                     RGGKASLIKLRKKETLVALKAAKSMGLKVAGVDMLQSKRGPLVLEVNSSPGLEGIENA
                     TKVDIAGKIIEYIEKEVTKKKRTKDKVKA"
     gene            10712..11656
                     /locus_tag="VGA21_11700"
     CDS             10712..11656
                     /locus_tag="VGA21_11700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009582051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HEX9651218.1"
                     /translation="MKEFIKINGEKIGVGEEKQINVPIARLPTHTQIHIPIYVSRSTE
                     DGPTLLVMAGMHGDEINGVETLRRILVRGLNRPKRGSVISIPVLNIFGFINFSREVTD
                     GKDVNRSFPGTTAGSLASQVAHYVTHEILPQINYGLDFHTGGARRNNYPQVRAALSDL
                     NNEELARAYNPPFIINAPYRDKSLRKQAAKEGIPVLVFEGGESLRLRKNAIDEGLNGF
                     LRLMKHLNMIDDAPEAAKTPVRIDKTTWLRAKVSGLQHTYCRNGCPVAKDEVIGLITD
                     PFGEFEAKITSPVDGFIIGINNNPVINRGDPLIHIGLK"
     gene            11725..12363
                     /locus_tag="VGA21_11705"
     CDS             11725..12363
                     /locus_tag="VGA21_11705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007660835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HEX9651219.1"
                     /translation="MEVFLGTLSALFSVVNPIGATPVFISLTQENSKKEIKDIALRTA
                     IYMVLILVIFFLAGKFILDFFGILVEHIRIAGGIMIVISGMRLLRQDSEKGKIISKEV
                     RLEGMEKDDISFTPMAMPLLSGPGSIALLIGMFDSLKGPAYWESGFIILAIILVSALT
                     FIILLFSRTLNKFLGKGGMAALSRLMGFIVLSIGIGMIMNGLIPLLKQINVN"
     gene            12400..12681
                     /locus_tag="VGA21_11710"
     CDS             12400..12681
                     /locus_tag="VGA21_11710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651220.1"
                     /translation="MKNFDRFQNVMSYYLRSINASIDLLKLKSNEVSGKKHKEMDKQI
                     KDLETHLDKVMEKLDEFFNADPDLLVEVEKELEEIYVDAKSSTRQAMSG"
     gene            12798..13370
                     /locus_tag="VGA21_11715"
     CDS             12798..13370
                     /locus_tag="VGA21_11715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008203182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-70 factor"
                     /protein_id="HEX9651221.1"
                     /translation="MTEAKGLTTEALKGGQIKAFEMFFRQHYNHLCNYANSFVKDRDE
                     AEEIVQNSFSSLWEKRESLNITGSVRSYIFSMVHNQCLNFLKHKKVKRKHASEMMNVT
                     LEANEDPDLQANELELRIFKALQSLPEKCGMVFKLSRFEELKYAEIAEKMNISVKTVE
                     NQMGKALKLMREQLRDYLPFILIVLSAWNG"
     gene            13358..14371
                     /locus_tag="VGA21_11720"
     CDS             13358..14371
                     /locus_tag="VGA21_11720"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HEX9651222.1"
                     /translation="MERMMEEENYMDILIAGFLAGETTPEQNQELNKWLETSEQNRKY
                     FENHKLIFSTSSRLKNWREFDANKAWRKLHIDSKADGRAIPLRRNSTTWLAVAASIAA
                     IATFGYLLLDQQNVTLEPVLWSTNTLTEVKRDTLPDGTLAFLNKETEIAFAYNETEKL
                     KTVALLGEAYFEIEKEKEERFQIEIDELIIEDIGTSFNVNAYPKSELITVIVEKGEVA
                     LYTLNDPGIRVLAGETGIFNKTTKSFAKISEADPNALAYKTRYFSFNNTLLSNIVASM
                     EEVYDVKILLENEMLRNCRITVTFRDEEIDTIMDIIAATLELEVRKINTTEFELHGNG
                     CVE"
     gene            14409..16223
                     /locus_tag="VGA21_11725"
     CDS             14409..16223
                     /locus_tag="VGA21_11725"
                     /inference="COORDINATES: protein motif:HMM:NF025092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEX9651223.1"
                     /translation="MAASQAQNSQKSPLEREVTLSFNFDKADGALRKIEQQAQINFSY
                     NPSIFDLKKDITAQFENKSVREILNSLFPGQLEFKEKGNYVILTKSQLAKNESKQPPL
                     IITGYVIDSDSGLKIGAVSIYDKRTLSAAITNRYGYFKIEINKPEDFSELTISKKGYA
                     DKTITVSKNSEEFLTISIDSEITGIDPVTQLPPDEPETSSALRDVVKPVAMTEREINM
                     TNIKDTLYRKFQVSVFPFVGTNGALSGNVVNEYSLNIFGGYSMGTSKFEVGGYFNINR
                     AYMHGTQLAGFLNYVGGDASGFQAAGFTNIVRGESHAWQMAGSTNVNFDSASKPQFAG
                     LLNTNLNNSKSLHMAGLANFHSGYYDGVAISGLLNANFKKSKGTLAAGMANFQIGDYH
                     GFQVSGLTNLNFGRMEGVQLTSLLNISGNLRGSQIGLVNIADSVSGVQIGFISFSRAG
                     YHKIEISADEIFYANLALRTGSSNAFYNIFTAGIKPQSGEPYWTYGYGVGSSPRLAKW
                     LYLNTDLTAHQVSKGNPVDEFHLLSKFYLGLEFQVARKFSITGAATLNSYLTKNTYTD
                     YPYLFTDYTPKIILEENYSSADLNHQMWMGWKFGLRFL"
     gene            16300..18657
                     /locus_tag="VGA21_11730"
     CDS             16300..18657
                     /locus_tag="VGA21_11730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010852947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX9651224.1"
                     /translation="MPKNLFIPVLFIALNFESSLSQELTQTIRGKVVDKVTNQELIGA
                     TVVIMGTDPIQGAITDITGRFRITKVQIGSHNVKISYIGYQEFILYNLVVNSGKELVL
                     NIPLEEEIIKMEEIVIRPEVDKEKPLNELAAVSARTFSVEETQKFAGSFNDPGRMVAS
                     FAGVTQTDDGNNMISIRGNSPYGLQWKMEGVEIPNPNHFGRVGSSGGGVSVLSSQLLS
                     NSDFLTGAFPAEYGNALAGVFDLNLRKGNNEQREYTLQAGFLGIDLAAEGPFSKRYKG
                     SYLVNYRYSTLSLLSQLGVPIGDAIINFQDISYNVFIPSGKSGSFSFFGFGGLSDQNF
                     DAPRDSAKWNESYDRYDFNYHTNTGMAGLKHAITIKDKTFLQTSLVYSGFGKGYVEDR
                     ISDTYMAERKYKDNSVEEKINANLVMNNKMNAQHSLRSGIYVTNISYSLVQKEDLNLT
                     GQMLDVINSNGNTQILQAFSQWNYRINQNLTFNLGFHYLNLFLNNTYSIEPRSSLQYQ
                     LNEKQSVSLGYGLHSQVQPIGVYFGQLQTAEGIILPNKDLKLSKSHHIVLSYDRTLKE
                     HLRLKTEAYYQYLFDIPISADPAGNFSLINSEGNYATEPLVNDGVGRNYGLEVTFEQF
                     MHRNLYYLFSGSLYDAKYKAHGEDWINTRFNGNFTLTFTGGKEFQILRNNKYRVLGIN
                     LKTIYTGGFRTTPIDIQQSIAEGQTIWMEDKAFTGRATNYFRTDVRISWKRNRLKSTS
                     IVSLDIQNVTNNQNIYGEYFDAESGTIEKWYQAPLIPILSYILEF"
     gene            19005..19634
                     /locus_tag="VGA21_11735"
     CDS             19005..19634
                     /locus_tag="VGA21_11735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019987353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEX9651225.1"
                     /translation="MTDNKKIYKVAFGLAIFTIIYNLAEGMISTYLGFEDESLALFGF
                     GSDSFIEVISGLGIAQMILRIQNNPNSKKSNFERTALKITGSAFYILVFALVITSIYN
                     LWTGHKPLTTFWGVVISMISILVMWVLVVWKRSVGNQLNSAPILADANCTLVCVYMSI
                     ILLVSSGIYELFGISYIDSIGTLGLSYFSFKEGKECFEKANSDKNWCCD"
     gene            complement(19879..20556)
                     /locus_tag="VGA21_11740"
     CDS             complement(19879..20556)
                     /locus_tag="VGA21_11740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651226.1"
                     /translation="MNLKIILLILIIASNAALSQNLQVLLTNSKDIDPHRYEGVRGSP
                     YFFNKFVKGNIISNGAETFTDMILNYNAESSEFEIINEGKTVQLDKSIYMRVEILPEG
                     NPELELDFPIIFQRGFNMKWLKKFGQMVYEGTTIKLIRDYTVQISSNEIQDVGKTLQN
                     KRFVNLEEYFLLVEGELISIKLKKKKILNELEHSNQLEIYVKENKIDIETESGLRKIL
                     AYYETIK"
     gene            20816..22327
                     /locus_tag="VGA21_11745"
     CDS             20816..22327
                     /locus_tag="VGA21_11745"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020528825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HEX9651227.1"
                     /translation="MKARMKKFGSLLSAAILGSLITVGSFQYLEIGNQGKLTIEHIDG
                     TPSRGTRYPAGNGGELANLNFTSAAKKVTPAVVNIRSTMSQPRGNTSYRNLPDPFRDF
                     FGDEFNRRFFNPNQDGQNFQPQIGTGSGVIINSQGYIVTNNHVIDKADDLEVTLNDNR
                     SYKAKVIGTDPTTDLALIKIDETGLPTLPFVNSDEVQIGEWVMAVGNPFNLTSTVTAG
                     IVSAKGRSINILQDRYAIESFIQTDAAINPGNSGGALVDLNGGLVGINTAIASTTGSY
                     SGYGFAVPSNMVSKVVEDLLKYGTVQRGLLGITIRSVTSSLAKEKDLNLATGVYVDSV
                     SENSGARIAGILPGDVIIEVDGNTVQTSPELMELVARKRPGDVVNLKINRNGKERKMD
                     VLLKNREGTTAVVSKENLNILTTLGAEFVTIDKKIATQLNIKGGVQVKEIFAGKLAQN
                     TEVRKGFIITKINETEVTNVEQFKDLLESGKGGILIEGIYPDSNGKYYYALGL"
     gene            22554..23657
                     /locus_tag="VGA21_11750"
     CDS             22554..23657
                     /locus_tag="VGA21_11750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017730661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX9651228.1"
                     /translation="MNKFAKGTLIVVIVLVVAFLMARPKIMEFIDGEISSKESVESPA
                     NSQAQILNVEAVIARPRRVDNKISVTGSVLANESVEITSEISGKIIGIHFQEGQEVKK
                     GELLVSLNDDELKAQLEKLQYTQKLYRDTEFRQRKLLEREAISQEEYEQAITVVKTNT
                     ADIKIIEAQLAKTKITAPFDGVIGLRMVSLGAYVTSSQIISSLYSINPAKVEFSIPSK
                     YSSMVSGGMPISFTTDGSAEIFKGNVYALEPQIEVRTRTLKLKAISPNPQSHLLPGQF
                     ARIELILQTLENAIMLPTESVIPELDGHKVFLKKDGKVNPVSVEVGIRTDREIQILSG
                     VKEADTVLTSGSLQVRPGQPITVTINNNIAWQD"
     gene            23645..>24364
                     /locus_tag="VGA21_11755"
     CDS             23645..>24364
                     /locus_tag="VGA21_11755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009186096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX9651229.1"
                     /translation="MAGLASISIMRPVLAIVMSITIVIFGIIGFNFLGVREYPAVDPP
                     VITVSTGYIGANADIIETQITEPLESSINGISGIKSLTSVSRDGSSRITVEFGLEIDM
                     EAAANDVRDRVSRAVSSLPPDIDAPVVTKADANSFPIIFLNVTSQKRSLLELSAFAEN
                     FLKERFQTIPGVSEARVWGSKRYSMRLNMDPDKLAAYGLTPSDVLNAVNSENVELPSG
                     LVEGLFTELTVRTQGRLNHPQE"
BASE COUNT         7798 a         4548 c         5121 g         6897 t
ORIGIN      
        1 cctggaaaat ctgtatccgg ataaaaatac ttttgccgta attctaagtc atgaccctaa
       61 aattgacgat gcagcattag agattttgct gaaaaaagaa attccatata tcggagcttt
      121 gggcagcaaa aaaacccatg aaaaaagaaa gacacggtta ctggagaaag gatttactga
      181 agcggaaatt gccaggatac atggtccggt gggaataaac ataaatgctc aaactgcagg
      241 ggagattgcc ttgagcataa tggcggaaat tatagaggta aagaattctt agaaatctgc
      301 attaaaatgt aatgaagtta attaacaaag ccggataatt tgaacctcat ttcccagaac
      361 aatttctcag cattaataaa ccaataaacc attacatgat ggatcgctat tattccaaaa
      421 gccagtatta caagtatgga atggaaccat tctttgagtt gaaaatcgcg atgacagata
      481 aatcgattgc aacaaatctc aaataattct cttccgggaa taattctgcc tcgcttaaat
      541 aaaatgatac tataattttg ataagcaagt aaatgttaca tttgcgcatc ttcagatgaa
      601 cacaaagaat ccccttttca aagcatacct ggctctggca attgtctgtt ttttttgggg
      661 aaccacttac ctggcaatca ggattggagt gcaattcgta cccggattca tgatggctgg
      721 aatcaggaat ataatagccg gcctgattac ctgttcatac tttgttttca ttggaactaa
      781 gatgccggac agaaaacttt ggggaataat ttttgtcaga gctttactcc tgatagtgtt
      841 ggggaatgcc atggttcact gggcggagga atctgttgaa agcggacttg cggcaatcct
      901 tgccgctacc gtaccacttt gggtggcggg tataagcagt atcggattca gatcggtcac
      961 attgaacaag aaatccctgg ccgggttgtt acttggcttt gccggggtct tatgcatatt
     1021 tattaccgga aaggaagacc tgtttcattt atcatccatt tggggagttc tggcaatgat
     1081 gattgccgct ataggctggt cattgggtac cgtatattcc tccgtaaata aaattaactt
     1141 acctgtcatc tttacggcag gaatacaaat gctgctgggt ggaataattt caataatgat
     1201 tagtttttca attggtgagc aagtctcggt actggcaatt caatggcaag ggtggacggc
     1261 cattttttac ctggtagctt tcggttctat cctttcttac aactctttca tttatgcgct
     1321 ggataaatta cccgcaaccc tggtaggaat ttacgcatac ttaaaccccc tggtggcagt
     1381 atttctggga tggctgatac ttgatgaacg tatggacgca tgggttgcat tcgggattct
     1441 catcacttta tatggtgttt atttggtaaa taaaggcttc agcctgaggg ctattacccc
     1501 tgataaatag tcttgcggtc atttgtaacc tttttgcaaa caatgggtat acatacatag
     1561 gtccagccaa atccggaaat ccatgcttaa gaattatctg ctgattactc ttagaaatat
     1621 ccgcaaacaa aaactttaca gtttcatcaa cttaaccgga cttgcaaccg ggattgccgc
     1681 gtgtttgatc attttccttt ttataaggga tgaaaaatca tttgatgctt ttcataaaaa
     1741 aaatggcgta atttaccgcc tctgtgaagt gcaatctttt ccgggtacat taacacaaaa
     1801 tgttgctttg tctatgcctg ctatgggccc gcatatgtgg acggaattcc ctgagataac
     1861 ttcttttacc cgctattttg gaggcactga ttggattttc aatattggcg aaaaagcaat
     1921 caaagtaccg cagcttgccc gggttgattc caccttcctg cagatttttg attttgaatt
     1981 gataaccggt gacaaaacaa ctgccttaaa tgatccctac agcattctaa taacagaaga
     2041 aaccgctatc aaattttttg gcaccacgga agtgctgggc agacaggttg cgcgaggtga
     2101 agataactat caaatcacgg gcatccttaa gaatgttccg gaaaacagcc accttcagtt
     2161 tgatgctctg atttcaatga gttcaatcac ttctcaatca ccggagttta atgaccggtg
     2221 gggatcgaat tacctggtta cctattttat tattccagaa ggaacaaacc tggaagcaat
     2281 ggctgaaaga tttccagaat ttttggtttc aaaaatggga gatgagatta cggaactgta
     2341 tcagttgtat cttcagccgc tttcccaggt tcacttaggt tcgattgata ctcaacatga
     2401 ctatcgtaat tatcgcaagt ttgatggaaa atacattagc atatttatgg ttctagccgg
     2461 attcgtttta ttaattgccg gcattaactt tatgaatctc agtatttatc ggtcagtcag
     2521 caggtctaag gaagttggtt taaggaaatc attaggagca cagaaaacac aacttgtagc
     2581 tcagtttatt ggggagtcgg taattttttc ctgcctggct ttggttacgt cgctggccat
     2641 taccgctttg ctgattcctt ctctgaacct gatgacaggt cgcactatgg atcttgcttc
     2701 gatcttaact gaaccattcc cggtttttgc actccttgga ataacattcc tggtaggact
     2761 gctggcagga gtatatcccg cattttatgt atctactttt aatccggcga cagttttgaa
     2821 aggcggctcc tcgagttcaa aacaaagtgt tttcaaaagc ggattggtag tgtttcaatt
     2881 tgcgttagct attggaatga ttgcgggaac ttttatcact cttaatcaac tccggtatat
     2941 gaaaaacaaa gatcttggtt ttgataaatc ccgcattgta ttaattcctg caagccagga
     3001 gtcggataat aagtacagaa ctatttacaa tgagtttaaa aatttacccg gagtggatgg
     3061 agtaacggct acgggtcaaa gacttggtaa caatttgcat cagtggtcag cgcgggcgaa
     3121 gaatgaggag gaattattag acattaccat ttctcaagtc aacgtggata aggattttct
     3181 tgaagtgtac gatattgaat tgctggacgg ccgaactttc actgatcaaa ttcattccga
     3241 tacaagggga caatcctttt taataaatga gaagctggca aaagaactcg gttgggaaaa
     3301 cccggtcggc acacaacttg gctttagcgg tgtggattct attggcactg tgattggagt
     3361 ggtaaaaaat tttaattaca attccctcca ctatgatata aatacgctgg gtatttatat
     3421 gcgccccgat tggggatatg atgaaatttc agtaaaaata aaggctggtg ccggcgaaga
     3481 agtgtttacc ggcctgaaga agatctggga tcaaaatgta tctgacaggc catttgagta
     3541 ctcttacctg gatgaacatt ttctagctat ttatcgcgcc gatacccagg taacgaaggt
     3601 tgttgcatct atcgccaccc tcgctattct aattgccatg ttaggactat ttggtttgtc
     3661 agcacttagt actttaaatc gagttaaaga agtgggtatc cggaaagtac tgggagcttc
     3721 aggaaacagc ttgctaatgc tgctcactaa taagtttact ttattgattc tgagttcttt
     3781 tattattgcg gccccagtta cttatttctt tatgaaaggg tggcttgaaa actttgcttt
     3841 tagaattgaa ataacatttg ctcctttcct ggtggcagca ctgatatcct tcctggtggc
     3901 gttcatcacc gttggttatc atgcaataaa agcgctaagc gtaaacccga cagatattct
     3961 aaggtatgag tagagcaatt aacaattatt atcaattggc acattgataa tcatcggatc
     4021 ggaatccccg tctccgaatc ccagtctttc agttcaatga taggtcgctg gattgcaagg
     4081 ttatttgggt atgaaataat attcctgtct ttcgtttgca gctttatata aaacaaggtg
     4141 atttccagaa tccgtcctgt tatcgaatta gtgccgtcaa caattcttat aatagaacct
     4201 accttatacg tatagtaaaa aaacaaaatt actgatgagg ttagattgct gagtattgac
     4261 catgaagcaa aaatcgcgac tccaactatg gtcaggaatc ccaaaagata atcccgcaga
     4321 attctaaggt ttatatccca ggttagaacg atgaggacag tgatgatgag cagatttata
     4381 ctatgaaaaa gttttagaag gtacagagtt ctcttttctt caaactgatt ttttctggct
     4441 cttctcttta taattctcgt tgcaaaaaaa cgacttatta cataaagaac aacgaagaag
     4501 acagttacaa taactttgta atctttgaac cactccataa tttttctatt tggagttaca
     4561 aaaatagaga tcactccaat agactgaaag gaacctggat catttattta ttaacttgaa
     4621 gactttttgc ttagcacgct tcaatctcat tttaaccgca ctttcagaaa tgcccaaact
     4681 tgaatgaatg tctttaatag actgcttctc ctggtattta agtataagga tgagacgctc
     4741 ctccggggtt atctggttta acaattgggt aagaacctgg gttgtaattt ccggtgaaac
     4801 ctgttcttcc aggtcgggaa tatcctccgt gaggtcggcc agatgcttta tgataatcgt
     4861 attccggttg gcgctctttc gcaaatggtc tatgcaggta ttgtgaatga tagcatatag
     4921 ccaggttgaa aatttcgcct ccttcctgaa cctgggcaac tgaagataaa ccttaatcag
     4981 gatctcctgg gtaaggtccg ccgctgtttc cttttcctta atataagaag cgcacctttt
     5041 aaagatttcc ttttcgtatc ttgttacaag caaaggaaag aaccgggcgt tgccttcatg
     5101 ctgtaatagc tcaaccaggg cttcatctgt tagatctttc cttaccattt cggggtcaaa
     5161 tatactccat actaaaggtg actttttgct aaaagtccgt ctaaacacta acaataagat
     5221 gattgcaaaa gaaacaaagg cggtattaga attagacatt cagccgcaac ctactgaaga
     5281 gacttgtggt cccacctgcc tgcatgcggt ttataactat tatggtgaaa aaatatccct
     5341 gtctaccgtc ataagcgagg tcaaacaact tgcatcaggc ggaacgctgg gagttttgct
     5401 tggcacccat gcattggagc acggttttga tgtaaccatt tatacctata atcttgatat
     5461 atatgatccc acctggtttc atgccaacgt atcgatcccg gataaactga gagaacagct
     5521 taagtataag aaaggcaaga aattcaagtt tgcctcagaa gcctatttaa aatttctgga
     5581 aattggcgga cagattaagt tcaaagaact tactccagcc cttattacta actttattga
     5641 agaaggaatt ccggtgatct caggattaag cgttacctat ttgtaccaaa gtgccaggga
     5701 gattggagag aaaaatcttt atgatgatgt taaaggcgag cctgccggtc actttgtaat
     5761 tgtaaatggc tatgataagg aaacacgttc tgtttatatc gcggatcctt ataaacccaa
     5821 cccaatgatt gacggccatt tttataaagt gaaaacccac agattaacca atgcgatttt
     5881 gctgggcatc gtaacctatg atgccaacct gctggttatt cgaccaaaaa aataacagta
     5941 tgtcaaagct tgtaatagcc gaaaacccaa aaaagtggac tattcaccag gaagatgtgg
     6001 tagtaattac ccccaacgaa tactttagcg accctttgta tcagacctcc cgctacaaag
     6061 tgattaattt atgcaggtct taccaatatc aaaccctggg ttattatgtt tctttattgg
     6121 gtgaggcccg gaagcataaa attattccta aagtgtccac cttgcaggaa ttcagatttc
     6181 ccagcatagt cagagacgag acaggcgggt ttgaggaact aattcaaaat gaatttaaat
     6241 ctgtcaaaga aaaagaaatt gaactgaatg tttatttcgg attcacccat gacgcggagc
     6301 ataacaaaat tggaatactg ctctttaacc tttaccaggc accactcctg aaagcagtct
     6361 ttgaaaagca aaaggtatgg gagttacaaa gcttacgtcc gctccagtta aatgaaattg
     6421 atgataatga acggcctact ttgaatcaag cgcttgaatt gtacctcaaa ggaaggcagg
     6481 ttgtaagaaa aagcctttca aggaaaaaat acgacctcgc tatcatggta aatccggatg
     6541 atgctaaccc accctctgat gccaaagcac tccagaaatt tattaaagct gctggcaaag
     6601 aaggactgaa tgctgaaatc ataacaaaaa atgattttgc caagttagtt cagtatgatg
     6661 ccttattaat cagggaaact accgccgtga acaaccatac tttcaggttt gcaaagaagg
     6721 ccgtttctga aggcctggta gttattgatg atcctgattc gatgcttaga tgtaccaaca
     6781 aagtatttct gagtgaatta cttaaaaccc ataatatccc ttcacccaaa accgtaatac
     6841 tacaagattc aaaattaacc gatgagttac gtgaactcaa gtttcctttt attttgaaag
     6901 aaccagacag tgcttttagc aaaggagtaa aaaaagtaaa taatgaaacg gagctaaaag
     6961 aaactctgaa agatttcttc caaaaatcag accttgttat cgcccaggaa tttattccta
     7021 ccgactacga ctggcgggtt ggagtgctgg atgacgaacc aatttttgtt tgtaaatatt
     7081 ttatggcccg aaatcactgg caaatcatgg attggacaaa aaaagtcaga aaagaaggaa
     7141 agacagaaac aattgctgta gaggatgcac cgccaaaatt gattgacatt gcactcagag
     7201 cagccaaagc tgtgggcaat ggtttatatg gagtcgatgt taaagaaatt aataacaaat
     7261 tttacatcat tgaagtgaat gacaatccaa atattgattc ggggatagaa gacaaagtgc
     7321 tgaagctgga tttgtatcgt aaaataatgg ccaccattgt taaaagaata aaagcgagct
     7381 agtaattata tgccgaacat tcaaagatta cacctcttcc agggatatgg agttgaactg
     7441 gaatatatga ttgtggataa ggaaaccctt aacgtccggc ctttagccgc cgacttgctc
     7501 accaaagcaa tcggaagcct ggaggatgat ttcattaatg gagaaattac ctggtctaat
     7561 gaactggtaa tgcatgtgat tgagttgaaa tcaaccttgc ccgtaactga tatggtcaaa
     7621 ttctccactc acttcaaaaa taatattttg atggccaata atattcttga tgaatttaac
     7681 gccatgttga tgccaactgc cgcccatccc tggatgaatc cgcaaacaga tacggtattg
     7741 tggccttatg gaagcaagga aatttatgag acatataaca agatatttaa ttgtcagggt
     7801 cacggatggt caaatctgca aagctcacat ttgaaccttc ccttttatga tgatgaggaa
     7861 tttgcacgcc tgcatgcggc tgtgaggctt gttttaccaa tacttccggc acttgcggca
     7921 agttctccgg tgctggaagg aaagttttcg aaattctatg ataagcgact gagttattac
     7981 gagaagaacc aaatgagaat tccctccatc accggtaaag tgataccgga gaaattattt
     8041 tctcgtcgtt cttatcagaa attcatcttt gataaaatca aaactgatct tcaaaagcat
     8101 gatacgctgg gaattatgga tccggtgtgg gtgaattcaa gaggtgccac ggcaagattt
     8161 gatcgtggaa ctattgaaat cagactgctg gatattcagg aatgccccgc cgccgatttg
     8221 gctataatag cactggtagt acacactctg aaatggcttg tggctgaaga aggcataaaa
     8281 cttcatgaac aaatggagtt cggggtagaa ccgctttaca aaattttcaa tgccacaatc
     8341 agagatgcag aggaaaccat aatcaatgac ccaaaatttc tttccatctt caaaatagaa
     8401 aaatctgaac tcaaagcagg cgaactctgg aaagaaatcc actctatagt caaaggaaaa
     8461 tatccggatg aaatggttcc gtgggaaaat tacctgaaga atatttttga acagggaaca
     8521 ctcgcccgca gaattatgaa catagctggc aatgaaccaa ctctggaaac actcaaattg
     8581 gtttataaag aacttagtga ttgcctgcag gaaaatgaaa tgtttaactc atggatcgaa
     8641 gacgtattat aattagctgt gaacacgcag gtaacaaggt tccaacccag ttccggttct
     8701 tattcaaagg gaaggaatca gttttacaaa gtcacctggg ctgggatccc ggagcttttt
     8761 cggtcgccag aagtctggct gatgcattaa aagtcagatt attccatcag gattactccc
     8821 gtttgctcat cgaatgcaat cgatctcttg ataatcctga actgttcagt aaatacagta
     8881 ccggattaaa tgatatggtg aaggattatt taatacggaa gtattattta tcatatagaa
     8941 aaaaagtaga acgcgctatt ggtaaaatcc tggataaagg gaaacctgtt ttgcacttaa
     9001 gtattcattc gtttactcca gcacttaacg gaactaagcg taaagcagac ctggggttgt
     9061 tgtatgatcc cgaccgaaga tcagaagaag acttttctgt tcacctaaaa aatgaacttg
     9121 aaattctaag ccccaacctg ctcgtaaaat ttaactaccc atacctgggt actgatgacg
     9181 gattcactac ctatttaagg acgaaatttc ctgatgacag ctatgccgga attgaaattg
     9241 aagtaaatca gaacatgacc gcctggcctg ccaggaagaa aattcaaagg attttaactg
     9301 aatgtatcag aaaaatgtat tttgaggtaa ctcatgaatt aaactaatga ataatggatg
     9361 atcgaaaagt tattggcaga agtgataaaa ttgatctccc cgaatttggc attttcaacc
     9421 tggaggcgaa aatagacacc ggagcagcca tttgtgcaat tcattgccat gaaatacaat
     9481 tagtaaaaaa gggaaatgaa gaattgctgc agttccaatt gctggatcca tctcatcccg
     9541 gttacaatga aaaaacatat tattcacacc actttgaact tgcacgaata aaaaactctt
     9601 ttggccagac cgaagaacgt tatgtagtga ctaccccggt aatcattttc ggaaaaacaa
     9661 ttgaaacacg gttttctctt accgaccgga aagatcttaa atttccaata ttattaggca
     9721 gacaattttt gcgaaaacga tttatcgtgg atgtagcgaa aaaaaatatt tcatttaaaa
     9781 agaaaaaatg aaaaaactta agatagcggt tttatcgagg aatccgaaat tatacagtac
     9841 tcagcggttg gttgaagcaa taaccaccag ggggcatgag tctatggtga tagaccatct
     9901 caaatgcgat ttggttatgg acgaaaaagg tcctgccatt tattataagg gaatgaaact
     9961 tgaaaagatt gatgccgtaa ttccacgaat cggagcttcc gttacttttt atggatcagc
    10021 ggtggtaaga caatttgaaa tgatgcatgt ttttagcgca attgattcac tcgccattac
    10081 gcgttcccgt gataaattga gaagtctcca gatactttca cgcgctggtg tggggatgcc
    10141 caaaacggcg tttactaatt actcaagtga aaaaaatgaa attcttcatg agataggatc
    10201 acctcctgtg gtgataaaat tgctggaagg aacacaaggc ctcggagttg tgcttgcaga
    10261 aaccaggaaa gcagccaagt ctgtaattga ggccttcgaa ggcttaaaag cgcggataat
    10321 acttcaggaa tttattgaag aagccggagg ggctgacgtc agagttttca ttgttaatgg
    10381 cgtagtggta ggagcgatga aacgccaggg aaaagaagga gaattccgat caaatttaca
    10441 tagaggagga aaagcttctc ttattaagtt aagaaagaag gaaactcttg ttgctttgaa
    10501 agctgcaaaa tcaatgggtt taaaagtggc aggagtagac atgctgcaat caaagcgagg
    10561 gccgttggta cttgaagtaa attcatcacc cgggctggaa ggcattgaaa atgcgactaa
    10621 agtggatatt gccgggaaaa ttattgaata tattgaaaag gaagtaacaa agaaaaaaag
    10681 aaccaaagac aaagtgaaag cttgaaaaca aatgaaggaa tttataaaaa ttaatggaga
    10741 gaaaatagga gtgggggaag aaaaacagat taatgtgccg attgcacgat tacctaccca
    10801 cacccaaatc cacattccaa tttatgtgag cagatccacg gaagacggac ctacgctgct
    10861 ggtaatggca ggcatgcatg gcgatgaaat taatggcgta gaaaccctcc gcaggatttt
    10921 ggtccgtgga ttaaacaggc ccaaaagggg atctgtaatc agcattcctg ttcttaatat
    10981 tttcggcttt attaactttt ctcgtgaagt aactgatggc aaagatgtga atcgctcctt
    11041 tcccggaacc accgccggtt ccctggcatc acaagttgcg cattatgtta cccatgaaat
    11101 ccttccgcaa attaactatg gccttgactt tcataccggc ggcgccaggc gaaacaatta
    11161 tccgcaagtc agagcggctt tatccgattt aaacaatgag gaattagcgc gcgcatataa
    11221 ccctcccttc ataatcaatg cgccatacag agataaatcc ttaaggaagc aggctgcaaa
    11281 agaaggaatt ccggttctgg tttttgaagg aggcgaatcc ctgcgccttc ggaaaaatgc
    11341 tatcgatgaa ggtttaaatg ggttcctcag attaatgaag cacctgaata tgattgatga
    11401 cgcccccgag gccgctaaaa ctcctgtaag aattgacaaa actacctggt tgcgggcaaa
    11461 agtatccggg cttcaacaca cctattgtcg taacggctgc ccagttgcca aagatgaggt
    11521 aattggatta ataactgatc ccttcggaga gtttgaggcc aaaataactt ctccggttga
    11581 tgggtttatt atcgggatta ataacaatcc ggtaataaac cggggagatc cattaattca
    11641 tattggcctc aaataaaata aattttcaat tcttcaaatt gggtttaaaa aacttctaat
    11701 tttggcgaca aattcaggta aaaggtggaa gtatttctag gcactttatc ggcattattt
    11761 tcggtagtaa atcctatcgg ggcaacgcct gtttttatca gcctcaccca ggaaaattca
    11821 aaaaaggaga tcaaagatat tgccctgagg acagccattt acatggtgct gattttggtg
    11881 attttttttc tggctggaaa attcatcctg gattttttcg gtatcctggt ggaacatatc
    11941 agaattgccg gtggaatcat gatcgtgatc tcaggaatga ggttattgcg acaagactca
    12001 gagaaaggta aaatcatctc taaagaagtg aggctggaag gaatggaaaa agacgacatc
    12061 tcatttaccc caatggctat gcctttattg tcagggcccg gttccattgc cctgcttata
    12121 ggaatgttcg attcattgaa aggtccggcc tattgggaat ccggatttat tattttagcc
    12181 attatcctgg tttcagcttt aacatttatc atattattgt tttcccgaac tcttaacaaa
    12241 ttccttggaa aaggtggaat ggctgcgctc agcaggttga tgggattcat agttttatca
    12301 attggtatcg gcatgattat gaatggtttg ataccgcttc ttaagcagat aaatgtaaat
    12361 taagcttgaa ttcttaaatt ttataattcc cttaaaatca tgaaaaactt cgatcgattc
    12421 caaaatgtaa tgtcctatta cctcagatcc attaacgcga gtattgactt gcttaagcta
    12481 aaaagcaatg aggtgtcggg taaaaaacat aaggagatgg ataagcaaat aaaagacctg
    12541 gagacgcatc tggataaagt aatggaaaag ctggatgaat ttttcaatgc ggatccggat
    12601 ttgctggttg aagttgaaaa agagttggaa gaaatctatg tggacgccaa gagtagcacg
    12661 cggcaggcca tgagcggcta aatgaacaga tgataaatta attgtattat tcctaatatc
    12721 gtataaccgc aagtaggggt atttcaaggc cagattgtct tatagacaaa cctctctgaa
    12781 aacggtaatg ttaaccggtg actgaagcaa aaggattaac cacagaagct ttaaagggag
    12841 gccagataaa agcatttgaa atgtttttca ggcagcacta taatcaccta tgtaattacg
    12901 ccaactcatt cgtaaaagac cgggatgaag cggaagaaat tgttcaaaat tcattttcat
    12961 ctctctggga aaaacgtgag tccttaaaca ttaccggttc cgtaaggtct tacatatttt
    13021 ccatggtgca caaccagtgt ctgaatttcc taaaacataa aaaggttaag cggaaacatg
    13081 catctgaaat gatgaatgta acgctggaag ccaatgaaga tccggacttg caagccaacg
    13141 aacttgaact aagaattttc aaagcattgc aatcccttcc tgaaaaatgc gggatggttt
    13201 tcaaattgag ccggtttgaa gaactgaaat atgctgagat agcggaaaag atgaatatct
    13261 cggtaaagac tgttgagaac caaatgggaa aagcattaaa actgatgcgt gaacaattga
    13321 gggattacct gcctttcatt ttgatagtat taagtgcatg gaacggatga tggaagagga
    13381 aaactatatg gatatcttaa tagccggttt cctggcgggt gaaactacgc ctgagcaaaa
    13441 tcaggaatta aataaatggc ttgaaacaag tgaacaaaac aggaaatatt ttgaaaatca
    13501 taaactgatt ttttcaactt catcccggtt aaaaaactgg cgggaatttg atgcgaataa
    13561 ggcatggcgg aaactgcaca ttgactccaa agcagatggt cgggcaatcc ctttgcgaag
    13621 aaattccaca acctggcttg cagtggctgc atcaatagct gcaattgcta cattcggata
    13681 cctgcttttg gaccaacaga atgttacact cgaacctgta ttatggtcaa ccaatacctt
    13741 aaccgaagta aagcgggata ccttgcccga tggaacgctt gcctttctta acaaggaaac
    13801 ggaaatagcg tttgcctaca acgaaactga gaaattaaag acagtggcat tgctggggga
    13861 agcttatttt gaaatagaaa aggaaaaaga agaacgattt cagattgaaa tcgatgaatt
    13921 aattattgaa gatatcggaa cctccttcaa tgtaaatgcg tatcctaaaa gcgaattgat
    13981 tacagttatt gttgaaaaag gcgaagttgc cttatatacc ctaaacgatc caggtatccg
    14041 ggttttggca ggagagaccg ggattttcaa caaaacaacg aaatcatttg caaaaatcag
    14101 tgaagccgat ccaaatgccc tggcctataa aaccaggtat ttcagtttca ataatactct
    14161 gctgtcaaat atagtagctt ccatggaaga agtttatgat gtgaagattc tcctggaaaa
    14221 tgaaatgtta agaaactgca gaattacggt tactttccgg gatgaggaaa ttgatacgat
    14281 aatggatatc atagcagcaa cgctggaact tgaggttaga aagattaata ctactgaatt
    14341 tgaattgcat ggtaatggct gtgtcgagta aacaattttt gcttgctata attacgctct
    14401 gtttcttcat ggcagcatcc caggcgcaga atagtcaaaa atctccgctt gaacgcgaag
    14461 ttaccctcag ttttaacttt gataaagcag acggagcttt gcggaaaatt gagcaacaag
    14521 cacaaattaa tttctcctac aacccctcca tttttgatct taagaaagat ataaccgccc
    14581 agtttgaaaa taaatcagtt agggaaattc taaattccct gttcccgggc caattggaat
    14641 tcaaagaaaa aggaaactat gtgattctta caaagtcaca gttagcaaaa aatgagagca
    14701 aacaaccacc ccttattatt acgggttacg ttattgattc tgactccgga ttgaaaattg
    14761 gggcggtaag tatttatgat aaaaggacgt tgtctgccgc tatcaccaat cggtatggct
    14821 atttcaaaat agaaatcaat aagcccgaag actttagcga acttactatt agcaagaaag
    14881 gatatgctga taagacaata acggtaagta aaaattccga agaattttta acaatttcaa
    14941 ttgatagtga aatcactgga atagatccgg taacacaact gccaccggat gagcctgaaa
    15001 caagctccgc tttacgcgat gtagtgaaac ctgtagctat gacggagcgc gaaatcaaca
    15061 tgaccaatat taaagacact ttatatagaa aatttcaggt ttctgttttc ccttttgttg
    15121 gtacgaatgg agctctgagc ggtaatgtag taaatgaata ctcattaaat atttttggcg
    15181 gttactccat gggaacatca aaatttgaag tgggtggata cttcaacatc aaccgtgctt
    15241 acatgcatgg tactcagctt gcaggattct tgaattatgt cggcggagat gcatccggtt
    15301 ttcaggcagc gggtttcaca aatattgtaa gaggtgaatc acatgcatgg caaatggcag
    15361 gctccacaaa tgtaaacttt gattctgcat caaagccgca attcgcggga ctattaaata
    15421 ctaatctaaa taactcaaaa agtttgcaca tggcagggtt ggctaatttt cactccggct
    15481 attatgacgg cgtagcaata tcaggtctcc tcaatgcgaa tttcaagaag tcaaaaggaa
    15541 ctctggctgc cggaatggcc aattttcaaa taggagatta tcatggtttt caggtttccg
    15601 gtttaacgaa cctcaacttt ggtcgaatgg aaggagtaca attaacctcc ttgctaaaca
    15661 tttccggaaa cttgcgtggc tcccaaatcg gacttgtaaa tatcgcggac agcgtctctg
    15721 gtgttcagat tggctttata agtttttcga gagcaggata tcataagata gaaatttcag
    15781 ctgatgaaat attctatgca aatttggcac tcagaaccgg aagctcaaat gctttctata
    15841 acatctttac cgcgggaatt aaaccacagt caggcgaacc ttactggact tatggttatg
    15901 gcgttggttc atcccccaga cttgccaaat ggctatactt aaataccgat ttaactgctc
    15961 accaggtaag caaaggaaat ccggtggatg aatttcatct cctgagtaaa ttttacctgg
    16021 ggttggagtt tcaggtagcg cgaaaattct caattaccgg agcagctaca cttaacagct
    16081 acctgaccaa gaatacctac acagactatc catatctctt caccgattac actccaaaaa
    16141 ttattttgga agaaaattat tccagtgcgg accttaacca ccaaatgtgg atgggatgga
    16201 aattcggtct tagattcttg taaaaacttt tcaaataaaa taggggtctg aaagtccaga
    16261 gttgtcttaa agctgtaaaa gcaaaagact attagaatca tgcctaaaaa tttgttcatc
    16321 cccgttttat ttatcgcgct caattttgaa agttcccttt cccaggagtt aactcaaacc
    16381 attcggggaa aggttgtgga caaggtcacc aaccaggaat taattggcgc tacggttgtt
    16441 atcatgggaa ccgatcccat acagggtgcc attacagaca ttaccggacg tttcagaatc
    16501 accaaagttc aaataggttc tcacaatgtt aagatttcct acatcggcta tcaggaattt
    16561 atactttaca atcttgtggt gaattcaggt aaagaattgg tcttgaatat tcctctggaa
    16621 gaagaaatca taaaaatgga agagattgtc atccggccgg aggtggataa ggaaaagccg
    16681 ctcaacgaat tggcagcggt aagtgcaaga accttttcgg tggaggaaac acagaaattc
    16741 gcagggtcgt ttaatgatcc cggcagaatg gtagcatctt ttgccggtgt cacccaaact
    16801 gatgacggca ataacatgat ttccatccgc ggtaattcac cctatggcct tcaatggaaa
    16861 atggagggag tagagatacc taatcctaat cattttggaa gagtgggcag ctccggaggt
    16921 ggtgtttctg tccttagctc tcaattgctt tctaatagtg attttctcac cggcgcattt
    16981 ccagctgaat acggtaatgc tttggcggga gtttttgacc tcaatttaag aaaaggaaat
    17041 aatgaacagc gggaatatac gctacaggca ggttttcttg gaatcgacct cgctgcggaa
    17101 ggtccgtttt ccaaaaggta taagggttct tacctggtta attaccggta ttcaactttg
    17161 tcgctgctaa gccaattggg tgttcccatt ggcgatgcaa taataaattt ccaggatatt
    17221 tcatataacg tatttatacc atctggtaaa tcgggatcat tttctttctt cgggtttggt
    17281 ggactaagcg accaaaactt tgatgctccc agagattctg ctaaatggaa tgaatcctac
    17341 gaccgttatg attttaacta ccataccaat accggaatgg ccggactaaa acatgccata
    17401 acaataaagg acaaaacttt cttacaaaca tcattagttt attcaggttt tggtaaagga
    17461 tatgtagagg acagaataag tgatacctat atggcggaga gaaaatataa ggataatagc
    17521 gtagaagaaa agatcaacgc aaatttggtt atgaataata aaatgaatgc gcagcacagt
    17581 ttgcggtccg ggatttatgt aaccaacatt agctacagcc tggtacaaaa agaagatctt
    17641 aacctcacag gacaaatgtt agatgtaatt aattctaatg gaaacaccca aattctccag
    17701 gcgttctccc aatggaacta ccgaatcaac caaaacctta cctttaatct tggatttcat
    17761 tacctgaatt tatttttgaa taacacttat tctattgaac cacgatcctc cctgcagtat
    17821 caattgaatg aaaagcaatc tgttagcctg ggttacggac ttcattccca ggtacagccc
    17881 ataggcgtat attttggtca attacaaaca gccgaaggta taatcctacc taataaggat
    17941 ctgaaattat caaagtctca tcatatcgtg ctgagttatg atcgaacatt aaaagaacac
    18001 ctcagattga agacagaagc gtattatcag tacctgtttg atattccaat aagtgcagat
    18061 ccagccggca atttctctct gattaattca gaaggaaact acgccactga acctttggtg
    18121 aacgatggag taggaagaaa ctatggcctt gaagttacct tcgaacaatt tatgcacagg
    18181 aatctttact atttgttttc cggatcatta tacgatgcaa aatataaagc gcatggagag
    18241 gattggataa acacccgttt taatggtaac tttactttga cattcaccgg gggaaaggaa
    18301 tttcaaattc ttagaaataa taaatacaga gtactgggaa taaacctgaa aacaatttac
    18361 accgggggtt tccgaactac acccattgat attcaacaat ccattgcaga aggccaaacg
    18421 atttggatgg aagataaagc attcaccggg agagccacaa attatttcag aacagatgta
    18481 agaataagct ggaaaagaaa cagactaaaa tcaacctcca tagtgtcttt agatatccag
    18541 aatgtaacaa acaatcagaa tatttatggt gaatattttg atgcggagag tggaacaatt
    18601 gagaagtggt atcaggcgcc attaattccg attctaagtt acatactgga gttttgattt
    18661 gatggtcagg aacagcccct cttttttaat tgaaaactca attagagatt taaccgttgc
    18721 gatacaaaga gccccatgga cgaaatataa attgagagta aaactccgca tacccatcca
    18781 tttttaggaa atatctgtaa attagatatc cgcctttggt gttagatagc aaaacgttta
    18841 ccaaagcttg caggggttgg aaaatttata tctgcggttt aagccaaaag ggcgtttcgg
    18901 gagttgggag taaaacaatt acaaatagca tagttaaaaa aaagacaaac tgaagttttt
    18961 aaaacttatt gtccattcgg aaactccacc tggaaaaaat tcaaatgaca gataataaaa
    19021 aaatatacaa ggttgctttc gggcttgcca tatttacaat aatttacaac ttagctgaag
    19081 gtatgatttc aacatatctt ggatttgaag atgaaagctt ggctcttttt ggctttggtt
    19141 cggatagttt catagaggtt atctcgggcc ttggaattgc tcaaatgatt ttaagaatcc
    19201 aaaataatcc gaatagtaaa aaaagcaact ttgaacggac cgcacttaag attactggaa
    19261 gcgcttttta tattttggtt tttgcgctcg taataacaag catttataac ctttggacag
    19321 ggcacaaacc attgacaact ttttggggag ttgtaatttc aatgatttcc atcctggtta
    19381 tgtgggtttt ggtggtttgg aaaagaagcg taggaaatca attgaactct gccccgattt
    19441 tagcggatgc caactgcact ttagtatgtg tctatatgtc aataattctg cttgtaagta
    19501 gtggaattta tgaactgttt gggatttcat acatagacag catcggaaca cttggacttt
    19561 catacttttc ttttaaagaa ggaaaagaat gctttgaaaa agcaaatagc gacaaaaatt
    19621 ggtgttgcga ctgaatataa aaggctgcac acaacatata aagattaaac caagttagct
    19681 gcatagtaag ggactgcaac aatgccatta acttttatta gtggtgctcg ttgtaagctt
    19741 gtgccgaact gacttagaca gttcagcata gcttattttg gatattaatt gagcagatca
    19801 ttttattgag tgtgcgtagg taaaatgaca cgacaattat gctcgagaga agctgtattg
    19861 tcaaaatttc agaagaaatt attttatggt ttcgtagtaa gctaaaattt ttctgaggcc
    19921 cgattctgtt tctatatcga tcttgttttc tttcacatat atttccagct ggttggaatg
    19981 ttcaagctca ttcaggattt ttttcttttt taatttgata gaaatcaact caccctcaac
    20041 cagcaaaaaa tattcttcca aattcacaaa tcttttattt tgaagagtct taccgacatc
    20101 ctgaatctca ttagacgata tttgaacagt gtaatctctt ataagtttga tagttgttcc
    20161 ctcgtaaacc atttgaccaa atttctttag ccatttcatg ttaaaaccgc gctgaaaaat
    20221 tatcggaaaa tcaagttcca gttccgggtt accttccgga agtatttcaa ccctcatgta
    20281 aatggattta tcaagctgaa cggtctttcc ctcattaata atttcaaact ctgaagattc
    20341 tgcattatag tttaatatca tatcagtgaa agtctctgca ccgtttgaaa taatatttcc
    20401 ctttacaaat ttattaaaga agtaaggtga gcccctcaca ccttcataac ggtgaggatc
    20461 aatatccttg ctgttggtaa gtaatacctg aaggttctgt gataaagctg cgtttgaagc
    20521 aattataaga atcaacagaa tgattttcaa attcatgttg ttaagcagat ttctataaaa
    20581 atcaaaatta gaattttgca tttgaaagaa aataaagcaa tcctgctaat tcccaaggta
    20641 ctggaattct ggcaagattg ttaataatag ttaaaaaaga gccattttgg cgcaatcaat
    20701 cctgtttcct aagtcattct gacatcagat gccacacact caactcccta ttattgtcgg
    20761 tataattttg gcattcatgt tgcaatacaa attaccaaac tcaaattgta gaaaaatgaa
    20821 agcgcgtatg aaaaaatttg gttcgttact gtcagctgcc atcttgggga gtctgataac
    20881 agtaggtagt tttcaatacc ttgaaatagg aaatcaggga aaactaacca ttgaacatat
    20941 tgatggcaca cccagcaggg gaacccgtta ccctgcgggc aatggtggag aattggcaaa
    21001 cctgaacttt acttccgcgg caaaaaaggt aacaccggcg gtggttaata tcaggtctac
    21061 aatgagccag cccaggggaa acacttctta tagaaatctt cctgatccct tcagggattt
    21121 ctttggtgat gaatttaata gaagattttt taaccctaat caggatggtc agaatttcca
    21181 gcctcaaata ggaaccggtt caggagttat tattaattcc cagggttaca tagttacaaa
    21241 caaccacgtt attgataagg ctgatgatct ggaagtaaca ttaaatgata accgatcata
    21301 caaagccaaa gttattggta ccgatcctac cacagacctt gctctaatca aaatagatga
    21361 aaccggattg ccaacattac catttgtaaa ttctgatgaa gtgcaaattg gagaatgggt
    21421 aatggcggtt ggaaacccgt ttaatcttac cagtacggtc accgccggaa tagttagtgc
    21481 caaaggcaga agcatcaata ttttgcagga cagatatgcc attgaatcct tcattcaaac
    21541 cgatgcagcc atcaatcctg gtaacagtgg tggtgcactc gtggatttga acggcggctt
    21601 agtgggtatt aatactgcaa tagcttctac tacgggttca tattctggat atggttttgc
    21661 cgttccttca aatatggtta gcaaagtagt tgaagacttg ctaaagtacg gaacggtgca
    21721 gagaggtctg cttgggatta ccatccgaag tgttacttca agtcttgcaa aagaaaaaga
    21781 tttgaacctg gcaaccggtg tttacgtgga tagcgtttca gaaaatagtg gcgcccgaat
    21841 tgcagggata ctgcccggcg acgtgataat tgaagttgac ggcaatacag ttcagacatc
    21901 gcctgagtta atggaacttg ttgccagaaa gcgtcctggt gatgtagtga atttgaagat
    21961 aaatagaaac ggaaaagaac ggaaaatgga tgtattgcta aagaatagag aaggaactac
    22021 cgccgttgtt tcaaaagaaa atctcaatat ccttacaacc cttggagctg aatttgtcac
    22081 tatagataag aaaatcgcta ctcaattgaa cataaagggc ggcgttcagg ttaaggagat
    22141 ttttgccgga aaactagctc agaatactga agtaaggaaa ggattcatca ttacaaaaat
    22201 taatgaaacg gaggtaacaa atgtggagca gtttaaagat ctcctggaat ccggaaaagg
    22261 tggaattctg attgaaggta tttacccgga cagcaacggt aaatactact atgcattagg
    22321 gttgtagcca ataaagttga agcaaaaaag aaccccgctt caggtggttt ttttgttgaa
    22381 ccttctaaag aataagatta ccgtttatga taattaacgg ttctaatgtt tccgttttat
    22441 gtaaagttaa cctgcatttt ttctaatttt aagattcatt tgcctgcaaa aaaaaagtaa
    22501 taaatgttgt ccggcaggat ttgaaatttt gtctttaagt taaaagggag taaatgaaca
    22561 aattcgcaaa aggcacttta attgtggtaa ttgttttggt ggtggctttc ctgatggcgc
    22621 ggcctaaaat aatggaattt atagatggtg aaataagttc aaaagaatcg gttgaatcac
    22681 ctgcaaattc acaagctcag atattaaatg tagaagctgt aattgccagg ccaagaaggg
    22741 ttgataacaa aatttccgta accgggtcgg ttcttgccaa tgaatctgtc gaaataacgt
    22801 cagagatatc cggaaaaatc atcggcatac attttcagga aggacaggaa gtaaagaaag
    22861 gtgagcttct ggtgagcctt aatgatgatg agctgaaagc tcagctggaa aaattgcagt
    22921 acactcaaaa gctttaccgg gacaccgagt tcagacaaag aaaacttttg gaaagagaag
    22981 ctatttctca ggaagaatat gaacaagcga ttacagtagt aaagacaaat acagccgaca
    23041 taaaaattat tgaagcgcaa ttagcgaaaa caaaaattac agcaccattc gacggtgtaa
    23101 tcggtttaag aatggttagc ctgggagcat atgtaacttc aagccaaata atttcgtcgc
    23161 tgtacagcat taatccggca aaagtagaat tttcaattcc cagtaaatac agttcaatgg
    23221 tttcaggcgg aatgccaata agttttacca ccgatggaag cgccgaaata ttcaaaggaa
    23281 atgtttatgc gcttgagccg caaattgaag ttcgcaccag aactttaaaa ctcaaggcaa
    23341 taagtccaaa tccgcaaagc cacctccttc caggacaatt tgcccgcatt gagctgattc
    23401 tccaaacact ggagaatgca attatgttac ccactgaatc tgttatacct gaactcgatg
    23461 gacacaaagt ttttctaaag aaggatggaa aagttaaccc cgtctcggtc gaagttggaa
    23521 tccgaacaga cagggaaatt cagattttat ctggtgttaa ggaagcagac actgtgctaa
    23581 cttccggaag tttgcaagtt agaccaggtc agccgattac cgttaccatt aacaacaaca
    23641 tagcatggca ggattagcat cgatttcaat catgaggccg gttttggcaa tcgtgatgtc
    23701 cataacaatt gtcatcttcg gaattattgg attcaatttt ctgggagtaa gagaataccc
    23761 tgcagtagat ccaccggtaa tcacagtttc aaccggatat attggagcta atgcagatat
    23821 aattgaaact caaatcaccg agcctcttga gagttctata aatggaattt ccggaatcaa
    23881 aagcttaacc tcagtgagca gggatggatc ttcaagaatt actgttgagt ttggattgga
    23941 aatagatatg gaagctgcag ccaatgatgt ccgtgacaga gtttcacgcg cagtcagcag
    24001 ccttccaccg gatattgacg cgcccgtagt tacaaaagca gacgcaaatt cttttccaat
    24061 tatttttcta aatgtaacca gtcagaaacg ctccctgttg gaactgtccg catttgcaga
    24121 aaactttctc aaagaaagat tccagactat tcctggcgta agtgaagctc gcgtttgggg
    24181 ttcaaaaaga tactccatgc ggctcaatat ggatccggat aagctggcag cttatggact
    24241 aacaccttcc gatgtgttga atgcggtaaa tagtgaaaat gtcgaacttc cttccggtct
    24301 tgtggaaggt ctgtttactg aattaactgt cagaactcag ggaagactaa atcatccaca
    24361 ggaa
//
LOCUS       DASWHX010000180         5171 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_1298760, whole genome shotgun sequence.
ACCESSION   DASWHX010000180 DASWHX010000000
VERSION     DASWHX010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 5171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5171
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1298760"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(37..726)
                     /locus_tag="VGA21_11760"
     CDS             complement(37..726)
                     /locus_tag="VGA21_11760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019599897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX9651230.1"
                     /translation="MGNKVTCPISGKSYRKFLPYGRENPRANALCPDCLSLERHRLLW
                     LYMKDMTDFFTARKKLLHIAPEQCLMEKFRKMKNLEYVTADLESHLADLKFDLHNIPL
                     DNNSFDAIICNHVMEHVENDRVVMRELFRVMKPGGWGIFLIPLYFPLPEETIEDLAIT
                     DPIARERLFGQGDHVRKYGMDYMDRLKEVGFSVKADKYASYLSDEDIKRYALQRNEII
                     FFCQKDSGDLR"
     gene            964..1575
                     /locus_tag="VGA21_11765"
     CDS             964..1575
                     /locus_tag="VGA21_11765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002699736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX9651231.1"
                     /translation="MYAETLLDIAMTHADNLILKAAKGDIGAFNQLVNLWYKRIYNYA
                     LKYFCDHDMAMEISQKTFITVNLKLETLREPSKFKPWIYRVASNLCHEEERRRKRTHL
                     KVTFDTESDEVQNVEGTEYNPDRTFQQNELAEILLDALSLINEEQREIVIMKEYEGLK
                     FIEIAEILEISENTAKSRLYYGISALRKILVKKNVTKDTVYYG"
     gene            1568..2395
                     /locus_tag="VGA21_11770"
     CDS             1568..2395
                     /locus_tag="VGA21_11770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651232.1"
                     /translation="MARKWEKSDLISYLYGEIDPESKAELEVALNEDSGLQKDFNEIK
                     ETRDIMGRLGDKEVIPPQILFEGKESAISKLKPYRTWMAVAASILFVFLVGYLTDASI
                     KVSGNELTLAWGNEDSSERLLTREEMDQMINEKVKELQAGTNQDLERRFENFRDEVDV
                     RLVDLRNQNNKEIIRMVNNETSSQREELNLLVQNLKDQNLKSIAEFIEVNNKAQEQNV
                     QRLLADFSSFINRQRVEDLTIIDQNLRTLRDNSESKFEEAGVLLASIINSVNSTRNE"
     gene            2415..3560
                     /locus_tag="VGA21_11775"
     CDS             2415..3560
                     /locus_tag="VGA21_11775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651233.1"
                     /translation="MKNVKTSIWAFSLMLLTVTAWAQSYDAARMKTDLEIAEDILSRL
                     ANQDGERSFWGSNVNGTYIEGYGVILSLPSNFSSRQSVHWGIGSSGSATYTIVGGKRI
                     SIDQSKLDSLQEATRNMITQVMKTFLVDYADLINQLKAEDKIMVVQKKSDFSYAVISG
                     ERAVISEERDGGFYSIDEAKRNMGVSAEIRKSDLIDYKQGKISRDQAMGKVVMNYDLD
                     ASSKKEPDLELFSSIIGRAFRSDLTETYWTSSSPSYERLEGFGVIYNMRVYSRNNLGN
                     DYFSMPTLGEEKISREEGLKKVEEAYPKFEKAILNAMVEYGRTIRSLKEEENLMIKVQ
                     LTECKGCNMPESIDLAVKKSVLTQFDRGQLSLENAVKGVKITKYPYK"
     gene            3738..5147
                     /locus_tag="VGA21_11780"
     CDS             3738..5147
                     /locus_tag="VGA21_11780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017731615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX9651234.1"
                     /translation="MKKSILIAAGIVVTVLIGYFVVKGNKNKEAAEILATVKQGKFLV
                     EIETTGELEAKNSVQILGPAGIRNYRIFNLSIEKIIEEGTVVKKGDWVADLDKSELTN
                     KIKDAQSDLDQKESQYIQTQLDTALQMRQARNELVNLEYGVEEAEITLAQSQFEPPAT
                     VKQAEINLDKAKRAFQQARDNYKIKLQQNKAKMQEVAASLSKTRREYDGMLDVMKSFT
                     ITAPEDGMVIFRKGWDGKPIREGSQVNGWDPVVATLPDLNVMMSKTYVNEVDVRKIKP
                     GQPVEIGLDAFPEKKLRGMVINVANVGEQRPNSDAKVFQVNIQVRGSDPLLRPSMTTS
                     NNILTMELDSVLFIPLEALFNHNDSITFVYKKEGLRTTKQEVEVGETNANEAVILAGL
                     SSADKVFVSTPLGLENVPVNLIPEMDGKRLRKETEFEPIELEPMITLPNGQVVPASRF
                     RRPGQTPGSTSGARPQGDR"
BASE COUNT         1752 a          921 c         1150 g         1348 t
ORIGIN      
        1 catttattca attaaccgct tacggaattg ggtttattat cgaaggtctc cgctatcttt
       61 ttggcagaag aaaataattt catttctctg caaagcatat cgcttaatat cctcatcaga
      121 cagataggat gcatatttat cggctttcac actaaaacca acttccttta aacgatccat
      181 ataatccatt ccatatttgc gtacatgatc accttggcca aaaagccttt cccgggctat
      241 tggatccgta atggccaggt cttcaatcgt ttcttcaggt aagggaaaat ataacggtat
      301 cagaaaaatg ccccagcctc ccggcttcat tacgcgaaac aattcacgca tcacgaccct
      361 gtcattctca acatgctcca tcacatgatt gcagatgatt gcatcaaaag aattattatc
      421 caatggaata ttgtgcaaat caaattttag atctgccaga tgcgactcaa gatctgccgt
      481 tacatattcc aggtttttca tcttacggaa tttctccata agacattgct ccggagctat
      541 atgcagtaat tttttccggg cagtaaaaaa atcggtcata tccttcatat ataaccataa
      601 cagacgatgt ctttcaagac tcagacaatc gggacataat gcgtttgctc tcggattttc
      661 acgtccatag ggcaaaaact ttcggtaact ctttcctgaa ataggacaag taaccttgtt
      721 gcccatgtaa aatatcgcaa ctagccgaat cccagtaggc gccacccgct ggagaaatgg
      781 tctgggaaaa acccttagca ttaagcttat tagccttttc aatgcagaaa actcttattt
      841 ttaacctgat attggttcaa aaatgcctca aaataaggga taattgaatt atttgtaaat
      901 aaaatggcac acatagaact ttttgttctc agataccatc caagaagtgt aaaacagtag
      961 attatgtatg cagaaacttt gctggatatt gcaatgacac atgctgataa cctgattctc
     1021 aaagctgcta aaggggatat aggtgccttt aaccagctgg tgaatctttg gtacaagcga
     1081 atctacaatt atgcattaaa gtatttctgc gatcatgata tggctatgga aatatcgcaa
     1141 aagacattta ttacagtgaa tttaaaactt gagactttaa gagaaccgtc gaaattcaag
     1201 ccctggattt acagggtggc ttcgaatctt tgtcatgagg aggagcgaag aagaaaacgg
     1261 actcacctca aagtaacttt tgataccgaa agcgatgaag tacaaaatgt agaagggaca
     1321 gaatacaacc cggacaggac atttcagcaa aatgaattag ctgaaattct gttagatgcc
     1381 ttgagtttaa ttaatgagga acaaagagag attgtaataa tgaaagaata tgaaggattg
     1441 aaatttattg aaattgcgga aatccttgag atatcggaga acacagccaa atcgagattg
     1501 tattacggca ttagtgccct ccggaaaata cttgtaaaga aaaatgtaac taaagacaca
     1561 gtttattatg gctaggaaat gggaaaaaag tgatttgatt tcttacctgt atggggaaat
     1621 tgatccggaa agtaaagcag agttggaagt ggcactgaat gaagattccg gtctgcaaaa
     1681 agatttcaat gaaataaaag aaacccggga tattatggga aggcttggtg acaaggaagt
     1741 gattccaccc caaatattat ttgagggaaa agaatccgca atttcaaaat tgaaaccata
     1801 tcgcacctgg atggctgttg ccgcctccat actttttgta tttctcgtcg gttatcttac
     1861 ggatgcttct atcaaagttt ccggtaatga gttaacctta gcttggggca atgaagattc
     1921 ttccgaacga ttattgacca gggaagaaat ggatcagatg atcaatgaaa aagtaaagga
     1981 actgcaagcc gggactaatc aggatctgga gcgccgattc gagaatttta gagatgaagt
     2041 ggatgtaaga ctcgtagatc tccgaaatca aaacaacaag gaaattatca gaatggtaaa
     2101 taatgaaacc agttctcaaa gagaggagct taatctgctg gttcagaatt taaaggatca
     2161 aaacctgaaa tcaatcgcgg aattcattga ggttaataat aaggcacaag agcaaaatgt
     2221 gcagcgattg ctggctgact ttagttcgtt tattaaccgg cagcgcgttg aggatttaac
     2281 tattattgat caaaatctga ggacactccg ggataattca gaatcaaaat ttgaagaggc
     2341 cggtgtgctt ctggccagta taattaattc agtgaattca acaagaaatg aataagactt
     2401 taataattac taaaatgaaa aatgtaaaaa caagtatttg ggcgttttcc ttaatgctgc
     2461 tgacggtaac cgcgtgggcc caaagctacg atgctgccag aatgaaaaca gatctggaaa
     2521 ttgcggagga tattctttcc aggcttgcta atcaggatgg agaaagaagc ttctggggca
     2581 gtaatgtaaa tggcacttac attgaaggat atggtgtaat actctccttg cccagcaact
     2641 tctcttcaag acaaagtgtc cattggggaa taggttcatc cggatcagct acgtacacaa
     2701 tagtaggagg caagaggata agcattgatc aaagtaaatt ggatagcctg caagaagcga
     2761 ccaggaacat gatcactcag gtaatgaaaa cgtttcttgt agattatgcc gacctgatta
     2821 atcaattgaa agccgaagat aaaattatgg tggttcaaaa gaaatcagat tttagctatg
     2881 cagttatctc tggggaacga gcagttatct ctgaggaacg agacgggggt ttttattcca
     2941 tcgacgaggc gaagcggaat atgggagttt ctgctgaaat ccgtaaatca gacctgatcg
     3001 attacaagca aggaaaaata tccagggatc aggctatggg taaagtggta atgaattatg
     3061 atcttgatgc atcaagcaaa aaggagccag acctggaatt attctccagc ataattggcc
     3121 gggcattcag atcagattta acagaaactt actggacatc ttcttctcca tcatatgaaa
     3181 gactggaagg atttggagta atttataaca tgcgcgtata ttccagaaac aatctgggaa
     3241 atgattactt cagtatgcca actcttggtg aagaaaaaat ttccagggaa gaaggtctta
     3301 agaaagtaga agaagcgtat cccaaatttg aaaaggcaat actgaatgca atggttgaat
     3361 acggcaggac aatccgaagt ttgaaagaag aggagaatct gatgataaaa gtacaactaa
     3421 ccgaatgcaa gggttgtaat atgccggagt cgatcgatct tgcagtaaaa aaatctgtac
     3481 ttactcagtt tgatcggggt caactgagtt tagaaaatgc ggtaaagggt gtcaagatca
     3541 caaaatatcc atataagtaa aacagtgcaa gttcattcga aaaagtgcct ttagaaatac
     3601 tgaaggcact ttttcgtttt catccggata tatattttat aaatggtact tgcattattt
     3661 acatggtaca acactttatg aatttaaccg taactttgta aataaattaa ggttacaaca
     3721 ataacaacct gatcaaaatg aagaaaagta ttttaatcgc cgcgggtata gtcgtaacag
     3781 tactcatagg ctatttcgta gttaaaggaa ataagaataa ggaggccgct gaaatacttg
     3841 caactgtgaa gcagggtaag ttcctggttg aaatagaaac aaccggagaa ctggaggcaa
     3901 agaattcggt acagattttg ggtccggccg gcattcgtaa ttaccggata tttaatcttt
     3961 ccattgaaaa aattattgaa gaaggcactg tagtgaagaa aggggattgg gtggcagatc
     4021 tggataagtc ggagttgaca aataaaatca aggatgccca gtctgacctc gatcagaaag
     4081 aatctcagta tattcaaact cagctggata cagcactaca gatgcggcag gccaggaatg
     4141 agctggtaaa tcttgaatat ggagttgagg aagcggaaat taccctggcg caatcccaat
     4201 tcgaaccccc tgcaacagtg aaacaggcgg agatcaatct ggataaagca aaaagagcat
     4261 ttcaacaagc cagggacaat tacaaaatta agctccaaca aaataaggca aagatgcagg
     4321 aagttgccgc ctcattgtcc aaaacccgta gagaatatga cgggatgctg gatgtaatga
     4381 aatcattcac catcaccgca ccggaagatg gaatggtaat tttcagaaag ggatgggatg
     4441 gaaaacctat tagagaaggc tctcaggtaa acggatggga tcctgtggtt gccacacttc
     4501 cggatttgaa cgtaatgatg tcaaaaactt atgtcaacga agtggatgtt agaaaaatta
     4561 agcccggaca accggttgag attggccttg atgcgttccc ggaaaaaaaa ttgcgcggta
     4621 tggttataaa tgtggcaaat gtgggtgagc aacgaccaaa cagcgatgca aaggttttcc
     4681 aggtgaacat tcaggtgaga ggttcagatc cgctgctccg gccttcaatg acaaccagta
     4741 ataatattct caccatggag ctggacagcg ttttatttat tccattagag gctttattta
     4801 accacaatga ttcaatcaca tttgtctata agaaagaagg tctgcgaaca acaaagcagg
     4861 aggttgaagt gggcgaaact aacgcgaatg aagcagtaat tctggctgga ttaagtagtg
     4921 cagacaaagt ttttgtttcc acacccttag gtctggagaa tgttccagtg aatctaattc
     4981 ccgagatgga tggcaaaaga cttagaaagg aaacggaatt tgagccaatt gaattggagc
     5041 ccatgataac tttacccaac ggtcaggtag ttcctgcaag cagatttagg cggcctggtc
     5101 aaaccccagg aagtacatca ggtgcgcgtc cgcaaggaga tagataatta aagcaaagtg
     5161 tcggaaaggt t
//
LOCUS       DASWHX010000181        12170 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_1690183, whole genome shotgun sequence.
ACCESSION   DASWHX010000181 DASWHX010000000
VERSION     DASWHX010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 12170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12170
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1690183"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1944
                     /locus_tag="VGA21_11785"
     CDS             <1..1944
                     /locus_tag="VGA21_11785"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX9651235.1"
                     /translation="MEIQMGAEFQNFFVKAVLSDIPANSSLQFDYLISDLHYKTLYNP
                     RTVDSWTNVICETYILLDENNHAEALADKFPNLINEILPDFDQGTYSIGLQPITDIHL
                     NPDMPVGYAPVSDPKYSYILSAIAVLILVVAAVNFMTLSLGQSMVRAKEVGVRKVAGA
                     QRRQLIFQFLAEASLVTLISLGMGVLLSILGLPLFNDLAGKTLALNISGTTILFLGVL
                     AGLITFFAGGYPAFILSGFRPIMVLKNDVKVGGGKQTLRKLLVIVQFVFSIFLISSTL
                     IMRSQINFLRNKDLGFKKEQVLVVPLNISAGRYSAVVESGMQASKPFRNELMKLPEVV
                     KVGVASHIMGTGGWTGVGYTDSEGNFRNFDMNSIDEDYIPALQIEVKHGRNFDVNIPS
                     DIRRGIIINEAFAKEYAWEDPLIEKIPGNDFPDHEIIGVVKDFNYASLHGQVTPLVMV
                     MDPGIILKGISDIGIGTSIAPKLMISMSSDNLSESVEKIKAVWNKLSPGEEFNFSFVD
                     QTLDAQYENDRHLEQVVTSASILAILIGSLGLLGLASLNMTARVKEISMRKILGASNQ
                     TLLTLLAWDFLIMIAIALVLAVPVTYFAMSNWLNGFSYRVSITPVFFLAAGGISLLVA
                     MLTISYHAVKTTMTNPANNLRHE"
     gene            2043..3944
                     /locus_tag="VGA21_11790"
     CDS             2043..3944
                     /locus_tag="VGA21_11790"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013453503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HEX9651236.1"
                     /translation="MGNSVETLKKYWGFDAFRPLQQDIVESVADGKDVLALLPTGGGK
                     SICFQVPGMMREGLVIVISPLIALMKDQVENLKKRGIKAEAIVSGMKMREIDILLDNC
                     IYGKIKFLYVSPERLKTEIFLERLQKMNISFFAVDEAHCISQWGYDFRPPYLQIAEIR
                     SLKPDANIIALTATATPQVREDITRYLELKDPKVFVKSFSRANLSYSVRKTENKNEKM
                     LEILGKVAGTAIVYANTRRRTKELAEYLSQKGMNAEYYNAGLTMEQRISRQESWLKNK
                     KRIMVATNAFGMGIDKPDVRLVVHMDIPANPEAYYQEAGRGGRDEKKAYAVIVYHEND
                     LEDLIEKVKQGRPDLKSIQKVYQALANYFRVAVGSESEELFPFNIKEFCKNFNFNPIE
                     TAAAVRVLESEGIIATSEGYKNPSRLRIIIDQQTLYKKQVSDKTADKIVKGLLRLYGG
                     EVFNNYVNINEQDVSRLAEIEIIEITNKFTHLKEHGFIDYIPGSDKPSLGFVNRRYEA
                     TKLPMAAKQYEIRSKLEISKAESMVAYVSNDRRCRTQQLLDYFGEISYIPCGICDVCV
                     ARKENPVPIDTKLLKEKLGDKKISIPEFLDYWPKSKHEEIINQVRMLIDGGEIELSGA
                     NDIELVKKY"
     gene            4145..4639
                     /gene="tnpA"
                     /locus_tag="VGA21_11795"
     CDS             4145..4639
                     /gene="tnpA"
                     /locus_tag="VGA21_11795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010609444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HEX9651237.1"
                     /translation="MKIRGGPDEIRMGNKTMSTYTRILYQIVFSTKNRAPVLTATYRK
                     ELFKYIWGILDNKKCHLYQINGVEDHLHILTHLHPSIALSDLVKDIKLASTKLIKEKG
                     IFDGFTGWQVGYGAFTYAVDRKDALINYIKNQEAHHRTTTFREEYIDLLTEHGIPYEE
                     KYLL"
     gene            5049..5549
                     /locus_tag="VGA21_11800"
     CDS             5049..5549
                     /locus_tag="VGA21_11800"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4440 domain-containing protein"
                     /protein_id="HEX9651238.1"
                     /translation="MKTIQFISAVALACALASCSNQPGEPVSTFDLEEVKAMIGEANQ
                     KFMEAVDNGDSAAAATLYHSNAIIMPDNAEPVSGSDAILAFLGMIDNIGLKLESTSVW
                     GNEDVLVGEGVYEVTDTSGNSLDHGKYIVLWKEEDGQWKKYRDIWCSNVPMPMPEPAT
                     EEGAED"
     gene            complement(5800..6162)
                     /locus_tag="VGA21_11805"
     CDS             complement(5800..6162)
                     /locus_tag="VGA21_11805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018343187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family membrane protein"
                     /protein_id="HEX9651239.1"
                     /translation="MDSNVQLVKNVLKYTFGLVPIVAGLDKFTNILTDWSQYISEGFA
                     GVLPFEPSTFMMIVGVIEIAAGILVLTKTKIGAYVVSAWLVAIALTLIFSWSYVDVAV
                     RDLVMAIASFSLAKLSEK"
     gene            6284..6958
                     /locus_tag="VGA21_11810"
     CDS             6284..6958
                     /locus_tag="VGA21_11810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007658966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX9651240.1"
                     /translation="MEAINIYDSNNHKIKESEVIKRILGGEKELYEILVRRNNQKLYR
                     IIRSYLKDDVEIEDIMQNSYLKAFTKLYQFKLESSFSTWLIRIAINESLAQLKEKGKL
                     YYLNDSSGNLKSNTILEIPDNKQLNPQDKMIRNEAKQLLENAIDLLETKYRTVYIMKE
                     VEEMSLKEVSIALDITVANVKMRLHRSKEMLKDKLYNLSNNKNIFEFGFSRCDRVTEN
                     VMKLIR"
     gene            7334..8353
                     /locus_tag="VGA21_11815"
     CDS             7334..8353
                     /locus_tag="VGA21_11815"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEX9651241.1"
                     /translation="MEYKYFIGIDVSKNTLDISLLVSELGSAKHLQVSNSDNGILTML
                     KWLQELENYSVKESLFCLEHTGMYNYPLLQFFSKQSANVWVENPIQIKKSLGLQRGKN
                     DKVDALRIAQYAQRAKDRVKLWHPSREVVDKLRHLSALRERLVQTKLKLYTPVDELKK
                     VGNTPMAKVLEKSMSKTMKGLEKDLKAIEAQMKDVIDRDDNLRNLYALVTSVIGIGFV
                     TAVNMIVYTNEFKLFSNHRQFACYSGIAPFEYRSGTSIRGRTRVSHMANKKMKRQLHM
                     ASLTGIKFDEALKQYYERKVSSGKNKMSVLNAVRNKLVARVFAVVNRGIPYKKTNYQN
                     QLVLS"
     gene            complement(8519..9076)
                     /locus_tag="VGA21_11820"
     CDS             complement(8519..9076)
                     /locus_tag="VGA21_11820"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HEX9651242.1"
                     /translation="MKGLILAGGDGSRLGMEKAFLNLHGEIQVSYLCNQFRKSNIEPF
                     VSVPEKKRDQFQEYDLIFDKSDINGPLAGIVAAFDFHSGPWMVFAVDMPFINSEVMEY
                     FLKYRKPGVASCLTIDNINPEPMASIWESESYPLLQTYIADGGESASGFLKANGANLL
                     MVKDPNWVKSINTIEDLEWARQCID"
     gene            complement(9086..9562)
                     /gene="moaC"
                     /locus_tag="VGA21_11825"
     CDS             complement(9086..9562)
                     /gene="moaC"
                     /locus_tag="VGA21_11825"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014022759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HEX9651243.1"
                     /translation="MEKKLTHINKQGKPAMVDVSDKIVTLRTAKAESIVHFGKEIMKL
                     LDNGEIVSPKGPVFQTGIIAGTQAVKRTHELIPFCHPLPVTGIQIEIEPVSEEEVRVS
                     CTVKVEAKTGVEMEALMGASMTALTLYDMCKGLSHNIIISRIGLVEKTGGKRNFHR"
     gene            9609..10808
                     /locus_tag="VGA21_11830"
     CDS             9609..10808
                     /locus_tag="VGA21_11830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009581572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HEX9651244.1"
                     /translation="MIPVEEAYSVILDTVKITKSVAVDIKSCAGRIIREDIKADRNFP
                     PYHRVMMDGIAIQFENWKQGKKQFKIMGTQAAGTPMMESDSKECIEIMTGAMMPAQYD
                     TVIRYEDFVVSENGSDKIATINTEDIIQNQNVHAEGLDRRAGEVLIKKDTRISPAEVG
                     VIASVGETIIQVADFPKAALISTGDELVEINENPEAYQIRRSNVYSIHAVLKEMGMEA
                     SLFHFKDDSQQIRKELNQILNEHDLLIISGGVSKGKFDFLPEALRDSGVEQLFHKVNQ
                     RPGKPFWFGKKPEGVVVFGLPGNPVSTFLCFYKYIKPFLMASMKGSTAQQSAILAEDF
                     EINIPLTYFLQVKLTYENGNCIGRPVPGQGSGDFANLLDVDGFLELKPNAGAVKKGTI
                     LPLLPFR"
     gene            10866..>12170
                     /gene="ffh"
                     /locus_tag="VGA21_11835"
     CDS             10866..>12170
                     /gene="ffh"
                     /locus_tag="VGA21_11835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010610805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEX9651245.1"
                     /translation="MFENLSLKLDLAFKTLKGQGKITEINIASTVKEIRRALIDADVN
                     YKVAKEVTDSIKEKALGRDVLIAVSPGQLMTKIVYEELTELMGGSNAGINLTGDPAVI
                     LIAGLQGSGKTTFSAKLANYLSKQGRQVLLAACDIYRPAAIDQIKVLGESIGIEVYAE
                     PENKNALEIARNAIKHGKSTGKKTIIVDTAGRLAVDEAMMKEISSLKEILKPSETLFV
                     VDSMTGQDAVNTAKTFNEQINFDGVVLTKLDGDARGGAALSIRSVVEKPIKFISTGEK
                     IEALDLFHPDRMANRILGMGDVVSLVEKAQQTFDEEETRRLNKKFRKNQFDFDDFLSQ
                     LQQIKKMGNIKDMMGMIPGVGKAIKDIDVDDESFKPIEAIIRSMTLQERQNPDLIDGN
                     RRKRIANGSGTSVQQVNNLMKQFGEMRKMMKQMNKMGGAKKLA"
BASE COUNT         3912 a         2192 c         2632 g         3434 t
ORIGIN      
        1 atggaaattc aaatgggagc agaatttcag aatttctttg tgaaagcagt tctcagtgat
       61 attcctgcca attcaagttt gcaatttgat tatctaattt cagacctgca ttataaaacg
      121 ctatataatc caagaactgt agatagctgg acaaatgtaa tctgtgaaac atatatattg
      181 cttgatgaga ataatcacgc tgaggcactt gcagataaat ttcccaatct gataaatgag
      241 attcttcctg attttgatca aggaacttac tctatcggac tgcagcctat tacggatata
      301 catctgaatc ctgacatgcc ggttggatac gcgccggttt ctgacccgaa atattcatat
      361 atcctgtcgg caatagccgt acttatatta gttgttgcgg cagttaactt tatgacgctt
      421 tcattaggcc aatctatggt ccgtgctaaa gaagttggag tgcgcaaagt tgcaggcgct
      481 caaagacgtc aactgatatt tcaatttctg gcagaggcga gccttgtaac tctcatcagc
      541 cttgggatgg gggttttgct ttctatatta ggtttacctc ttttcaatga tttggccggg
      601 aagaccctgg ccttgaatat ttccggtacc actattttgt ttcttggagt gctggcaggt
      661 ctaattacct tttttgcagg aggctatccg gcctttattt tatccggatt cagacctatc
      721 atggttttaa agaatgatgt aaaagttggc ggaggaaagc aaacgctgag gaaattgctg
      781 gtgattgtgc aatttgtgtt ctccatattt ttaatctcca gtacacttat tatgcgttcg
      841 caaataaatt ttcttcgaaa taaagattta gggtttaaaa aagaacaagt actagttgta
      901 cctctaaaca taagtgcggg ccgctattcg gcagtggttg agtccgggat gcaggcatcg
      961 aaaccattta gaaatgaatt gatgaaatta cctgaagtcg tcaaggttgg agttgccagt
     1021 catattatgg gcaccggtgg ttggacaggt gtgggatata cggattctga ggggaatttc
     1081 agaaatttcg atatgaatag tattgatgaa gattatatcc cggctctgca aattgaagtt
     1141 aaacatggta gaaattttga tgtcaatatc ccttccgata taagacgtgg aataattatc
     1201 aatgaggcat ttgccaagga atatgcctgg gaagatccat taattgaaaa aattcccggc
     1261 aatgatttcc cggatcatga aataattgga gtggtaaagg attttaacta tgcttccctt
     1321 catggtcagg tgaccccact tgtcatggtg atggatcctg gaataatctt aaaaggtatc
     1381 tcggatatag gaattggaac ctcaatcgca ccgaaattaa tgatttctat gagcagtgat
     1441 aatctgagtg aatccgtaga aaaaataaaa gccgtttgga acaagctcag tccaggcgaa
     1501 gaatttaact tttctttcgt tgatcaaacc ctggatgctc aatacgaaaa tgatcggcac
     1561 ctggaacagg tggttacttc tgcatccatt ctggcaatcc tgattggcag tttaggtttg
     1621 cttggacttg cttcgctaaa tatgacagca agggtgaaag aaatcagcat gcgtaaaatc
     1681 cttggtgcat ctaatcagac attgctcact ctgcttgcct gggatttcct gatcatgatt
     1741 gcaattgcat tggtattggc agttccggta acctattttg ctatgagtaa ttggttgaat
     1801 ggattttcat accgggtatc cattacaccg gtattttttc tggctgccgg aggtatttct
     1861 ttattggtag ccatgctaac aatctcttac catgcggtga agacaactat gacgaatcct
     1921 gcgaataatt taaggcacga ataataattt aatcattatc gataatcccg gcccaggaga
     1981 ataagagata ttttattttt gaatatggcc tatcttagtt gcaaaaaaga agactggcct
     2041 ttatgggcaa ttcagttgaa acactaaaaa aatattgggg gtttgatgcc ttccgtcctt
     2101 tgcagcagga tattgtagaa tccgttgctg atggaaagga tgtcctggct ttgcttccca
     2161 cgggaggtgg taaatctatc tgtttccagg ttccgggaat gatgcgggaa ggattggtga
     2221 ttgttatctc acccttgatt gccttaatga aggaccaggt ggaaaacctg aagaaaagag
     2281 gaatcaaagc ggaagcgatc gtttcgggaa tgaaaatgcg ggagatagat atcctccttg
     2341 acaattgtat ctatggaaaa atcaaatttc tttatgtttc acccgaaaga cttaaaacag
     2401 aaatttttct tgaacgattg caaaagatga atatttcatt ttttgccgta gatgaagctc
     2461 actgtatctc tcaatggggt tatgattttc gccctcctta tttgcagata gcagaaatac
     2521 gatcattgaa gccggacgcg aatataatcg cgctaaccgc tacggccact ccgcaggtaa
     2581 gggaagacat aaccaggtac cttgagctaa aagatccgaa agtatttgtt aagagtttta
     2641 gcagggcaaa tctttcttat tcagttcgaa aaacagaaaa taagaatgaa aaaatgttgg
     2701 aaatattggg aaaggttgcc ggcactgcta tcgtgtatgc caatacaaga cgaagaacaa
     2761 aagaattggc tgaatatctt tcacaaaaag ggatgaatgc ggaatactac aatgccggat
     2821 taaccatgga gcaaagaatt tcccgacagg agtcctggct gaaaaataag aaaaggatta
     2881 tggtggctac gaatgctttt ggaatgggga tagataaacc tgacgtgcgc ctggtggttc
     2941 acatggatat tccggcaaat cctgaagctt actatcagga ggcgggaaga ggaggtcgtg
     3001 acgaaaaaaa ggcctacgcg gtaattgttt atcacgaaaa tgatttggag gatcttattg
     3061 aaaaagtaaa gcagggaaga ccagatctaa aatccatcca aaaagtttac caggcgcttg
     3121 ccaattattt ccgggtagct gtcggttcag aatccgaaga attattccca ttcaacatta
     3181 aagaattttg caaaaatttt aattttaatc ctattgagac ggcagcagca gttcgggtac
     3241 ttgaaagtga aggaataata gcaacttctg aaggatataa aaatccttca cgacttagaa
     3301 ttatcattga tcagcaaaca ttgtataaaa aacaagtgag tgataaaacc gcggataaga
     3361 ttgtaaaagg tttgcttcga ctctatggcg gggaggtatt taacaattat gtaaatatta
     3421 atgaacagga tgtaagccga cttgctgaaa ttgaaatcat agaaatcaca aataaattta
     3481 ctcatttaaa agaacacggg tttatcgatt atattcccgg tagtgataaa ccttcgctgg
     3541 gctttgtaaa ccgaagatat gaagctacga aacttccaat ggctgctaaa cagtatgaaa
     3601 tccggtctaa gctggaaatc agtaaggctg aaagtatggt ggcttatgtt tccaatgatc
     3661 gccgttgccg gactcagcag ttgctggatt attttggaga aatatcttat ataccctgcg
     3721 gaatctgtga tgtatgtgtg gccagaaaag aaaacccggt tcctattgat acaaaactgc
     3781 tcaaggaaaa actaggtgat aaaaaaattt ccatcccgga attccttgat tactggccaa
     3841 aatcgaaaca cgaagaaatt ataaaccagg taagaatgct aatagatgga ggagagatag
     3901 agttgtctgg tgcaaatgat attgaattag tgaaaaagta ttgaatttat ataaagtact
     3961 tcctgaggtt gaggtatgga tagaaaaaat cctgaaagga tttaatataa ataaccccgg
     4021 atgaaatccg gggataaaaa aagatgagat acctgaaccg cgaagcggtt caatagagaa
     4081 gaatcagaat cctgaaaggg agaagtatca aaatcctgaa aggatttaat ataaataacc
     4141 ccggatgaaa atccggggag gaccggatga aatccggatg ggaaacaaaa ctatgagcac
     4201 ctacacccgg atactatatc agattgtgtt tagcacaaaa aacagagcgc ccgtattgac
     4261 cgccacatac cgcaaagaac tttttaaata catctgggga atcctggaca acaaaaagtg
     4321 tcacctttat caaatcaatg gcgtagaaga tcacctccac atccttactc atttacatcc
     4381 ttccatcgcc ctttccgatc tggtgaagga catcaaattg gcaagcacca agttaattaa
     4441 agagaaagga atatttgatg ggtttaccgg ttggcaagtt ggctacggcg cttttactta
     4501 tgctgtagac cggaaagatg cattgatcaa ttacataaag aaccaggaag cgcatcaccg
     4561 gacaacaacc tttcgtgagg agtacattga cttactgacg gaacatggta ttccatatga
     4621 agaaaagtat ttgttatagt tcaacccctg cggggttgtt atcagggatt ttggtttgca
     4681 cggcatttca tgccgcgtta ttaaaaatta aatcccttcg ggattaatcc ggattgcaat
     4741 gggaaaaaga aaacatcaga aaaaggacag aaagacctct tcaacattca gcagttgaac
     4801 atggccgctt acgttcccca tatgaagaaa agtatttgtt atagttcaac ccctgcgggg
     4861 ttgttatctg ggattttggt ttgcacggca tttcatgccg tgttattaaa aattaaatcc
     4921 cttcgggatt aatccggatt gctatgggaa aaagaaatga ctagcccttt tatttctacg
     4981 ttttcccttg tcgttcaatt cacttttcca taacttatcc agttatccaa atgtaatcaa
     5041 accaaccaat gaaaaccatc caatttattt cggcagttgc actggcatgt gcacttgcct
     5101 cttgcagtaa ccagcctgga gaaccagttt caacttttga tctggaagaa gtaaaggcaa
     5161 tgattgggga agccaatcaa aagtttatgg aagccgttga taatggagat tcagctgcgg
     5221 ctgctactct ctatcatagc aatgccatca tcatgccaga caacgctgaa cctgtttcag
     5281 gaagtgatgc aatcctggca tttttaggaa tgatagacaa tatcggtctg aagcttgagt
     5341 ctacttcggt atggggcaat gaggatgtgc tggttggaga aggtgtttat gaagttaccg
     5401 atacaagcgg caacagcctg gatcatggaa agtatatcgt tctatggaaa gaggaagatg
     5461 ggcagtggaa gaagtatcgc gatatctggt gttcgaatgt gccgatgcct atgcctgagc
     5521 cggccactga agaaggagcg gaagattaag cctgctcttc gttataatct taactctggc
     5581 ttaactctgg tgcggaagat acttggcttc agccaccggt taggtgagag aaaaaaaatt
     5641 ttctgatctg aacattgaag tctccgcggg gtttcctcgc cgggataaga actgtttcaa
     5701 agcgaaaaac cccgcaggga ctattgttag ttcatatgtg aggttttgga aatgcttaaa
     5761 tttccaactt cattttatta attttgggct ttactaaaat tatttttccg atagtttagc
     5821 taaactaaaa gaagcaattg ccataactaa atctcttaca gcaacatcta cataactcca
     5881 gctaaaaatt aatgttaatg caatagctac taaccaagca gaaaccacat aagcaccaat
     5941 ttttgttttt gtcaacacca aaatacctgc tgcaatttcg ataacaccaa ctatcatcat
     6001 aaaggtagaa ggctcaaaag ggagcactcc agcaaaacct tccgaaatat attggctcca
     6061 atcggttaaa atattggtga atttgtctaa tcctgcaacg attggcacta aaccaaaggt
     6121 gtattttaat acatttttta ccagttgtac gttcgaatcc ataattttac tttttagttg
     6181 cttataaatt tgttacaccc ttttgatgac ggattttaaa aaaggataca aaattttgta
     6241 actttttcta tgccccctta tctaaaggaa aagacataat aaaatggaag ctataaacat
     6301 ttatgattct aataatcata aaataaaaga atcggaagtt ataaagcgta ttctcggtgg
     6361 cgaaaaagaa ctctacgaaa ttttagtgag acggaataac caaaaattgt atcgaataat
     6421 tcgcagttat ttaaaagacg atgtagaaat tgaagatata atgcagaaca gttacctgaa
     6481 agcatttaca aagttgtatc aatttaaatt agaatcatca ttttctacct ggctaattcg
     6541 tattgcaata aatgaatctc tggctcaatt aaaagaaaaa gggaaattgt attatttaaa
     6601 cgactcatct ggtaatttaa agagcaacac tattttagaa attccagata ataaacaatt
     6661 aaatccgcaa gataaaatga tacggaatga agcaaagcaa ttattggaga acgctattga
     6721 ccttttagaa acaaaataca gaaccgttta tataatgaaa gaagtagaag aaatgagttt
     6781 gaaagaagtt agtatcgctt tagacattac cgttgcaaat gtaaaaatgc gtttacatcg
     6841 ttctaaagaa atgcttaaag acaaacttta caacttatca aataataaaa atatttttga
     6901 atttgggttt agcagatgtg acagagttac tgaaaatgtt atgaaactga taagataaat
     6961 ttgcctcggc attacattta aatggaagag ttatatgcaa aagagtatgc tgagtttatt
     7021 actgtaacct gcctggattg gaagcttgtt ttatccgacg atagggttca acaggttatt
     7081 tcacagtgtt tcgcttgttg gtgaaaagaa caccaacctt tagacttgaa atcaaaagaa
     7141 ttttttaaga atctttgaat aatcaaccaa tggacagcag tgaactataa caccaactaa
     7201 gtaacgactt aacctttgat gtagactgct gttgttttgt atatgagatg cttcaaatag
     7261 aaatttaggc attgaggcct attattaagg ttttaaagaa actcatagac aggttgatct
     7321 aaagcttcat aatatggaat acaagtactt tattggtatt gatgtgtcca agaatacttt
     7381 ggacattagt ttgctggttt ccgagttagg atcagcaaag catttacagg taagcaatag
     7441 tgataatggt atattaacta tgcttaaatg gcttcaagag ctagagaact attcagtaaa
     7501 agaatctctg ttttgtttgg aacatacggg catgtataat tacccactgc tacaattttt
     7561 ctccaaacaa tccgccaatg tatgggtaga aaatccgata cagattaaaa agtctctggg
     7621 tcttcaacgg ggaaaaaatg ataaagttga cgctttgcga attgctcaat atgctcaaag
     7681 agcaaaggat cgcgtaaagt tatggcatcc atccagagag gtggtggata agctaagaca
     7741 cctctcagcg ctccgtgaga ggctagttca aaccaagctg aaattatata ctccagttga
     7801 tgaactcaag aaagtgggca atacgccaat ggctaaagtt ttagagaagt ccatgagtaa
     7861 gacaatgaaa ggtttagaaa aggatcttaa agctatcgaa gctcagatga aagatgtaat
     7921 tgaccgggat gataatctta gaaatctgta cgcactggta acctcggtaa ttggaattgg
     7981 gtttgttact gcagtaaaca tgattgtgta cacgaacgaa tttaaactat tcagtaacca
     8041 caggcaattt gcatgctata gcggtatagc accatttgaa tatcggtctg gtactagcat
     8101 tcgtggtcga accagggtaa gccacatggc caacaagaaa atgaaaaggc agctgcatat
     8161 ggctagctta actggtatta aattcgatga agcattaaag caatattatg aacgaaaagt
     8221 atccagcggc aaaaacaaaa tgagtgtatt aaatgctgtt agaaataagc ttgtggctag
     8281 ggtttttgca gtagttaacc gaggtatacc ttataagaaa acaaactacc aaaatcagtt
     8341 ggttttatca tagaaatcaa gggcagccag ggtctcgaac ttcctagaaa ggaaatagct
     8401 aaattcgaag ttcgacactt agcgaaacgt atgatggtac atactccctg cctgcgtcta
     8461 tttgcaaaac gctatgaaac atttcccgct gaaaaattat agacagccaa cagagttctc
     8521 aatcaatgca ttgcctggcc cactcaagat cttcaatagt atttatgctt tttacccaat
     8581 tcggatcctt aaccattaaa agatttgctc cattggcctt taaaaacccg gatgcacttt
     8641 ctcctccatc cgcgatgtaa gtctgaagta gcggatatga ttccgactcc caaattgatg
     8701 ccataggttc aggattaata ttatcaattg ttaggcaact ggcaacgccg ggtttccggt
     8761 atttcagaaa atactccatc acttctgaat taataaacgg catatccacg gcaaaaacca
     8821 tccagggtcc ggaatgaaaa tcaaatgccg ccacaattcc cgccaacggt ccgttaatgt
     8881 cagacttatc aaaaatcaaa tcatattcct gaaactgatc acgctttttc tctggaacag
     8941 aaacaaaagg ttcaatattg gatttccgga attgattgca gagataggaa acctgaattt
     9001 caccatgaag attaagaaac gctttttcca ttcctaacct ggagccatcg ccacctgcca
     9061 gaatcaatcc cttcatggta attaattatc gatgaaaatt ccgcttgccg ccggtttttt
     9121 caaccagccc gatccgggaa atgattatgt tatgagaaag tcctttgcac atatcataaa
     9181 gcgttaacgc ggtcatagat gcccccatca aagcttccat ttcaacaccg gttttggctt
     9241 ctacttttac ggtacaactt accctgactt cttcctccga aacaggttct atttcaattt
     9301 ggattcctgt aaccggcagc gggtgacaaa aagggataag ttcatgagtg cgcttaaccg
     9361 cttgtgtgcc ggcaataatt cctgtttgaa atacaggtcc ttttggagaa acaatctcac
     9421 cattatccag gagtttcatt atttcttttc caaagtgaac tatactttcc gctttagctg
     9481 ttctcaaggt cactatcttg tcactaacat ccaccatggc cggtttgccc tgtttattga
     9541 tatgggtcag ctttttttcc atttgatgta tatttacctt tataggctaa agttaatcat
     9601 tgtaattcat gataccagtt gaagaggcgt attctgtgat tctggatact gtcaaaatta
     9661 ctaaatctgt agcggtagac attaagagtt gtgcaggcag aattattcgg gaggatataa
     9721 aagctgacag aaactttccg ccgtatcaca gggtgatgat ggatggaatt gctattcaat
     9781 ttgagaactg gaagcagggc aaaaaacaat ttaaaattat gggaactcag gcggcaggta
     9841 cgccaatgat ggaaagcgat tcaaaggagt gtattgaaat aatgaccggg gcgatgatgc
     9901 ctgcacaata tgatacggtg attcgatatg aagacttcgt agtttctgaa aatgggagtg
     9961 acaaaattgc aactataaat accgaagata ttattcaaaa tcaaaatgtg catgcagaag
    10021 gtttagatag aagagcaggg gaggtattaa ttaagaaaga cactcgaatt agcccggccg
    10081 aagtgggagt aatagcttca gtgggggaaa ctattatcca ggtagctgat tttccgaaag
    10141 cggccttaat ctctacaggt gatgaacttg tagaaatcaa tgaaaatccg gaagcgtatc
    10201 aaattcggcg gtcaaacgtt tattccattc acgcggttct aaaagaaatg ggaatggaag
    10261 caagcctgtt tcatttcaaa gatgattcac aacaaattcg taaggagctc aaccaaattc
    10321 tgaatgaaca tgatctgctg attataagtg gcggcgtttc taaaggtaag tttgatttcc
    10381 tgccggaagc attacgggat tcaggtgttg aacaactttt tcataaagtg aatcaaagac
    10441 ccgggaaacc cttttggttc ggaaagaagc cggagggtgt agttgttttt ggtttaccag
    10501 gaaatccggt ttcgacattc ctgtgttttt ataaatacat aaaacccttt ctcatggctt
    10561 ccatgaaagg gagtacagca cagcaatcag cgatccttgc tgaagatttt gaaatcaata
    10621 ttccgcttac ctatttcctg caggtgaagc ttacttatga aaacggaaac tgcattggcc
    10681 ggcccgtccc cggccagggc tccggagatt ttgccaatct gttggatgta gatggtttcc
    10741 ttgaattaaa acctaatgca ggagcggtaa aaaagggtac gatactgcca ttattgccct
    10801 tccgttagat cttgatttct tcttcaatct ggaatgcctt atttttgtat actaaataaa
    10861 ccattatgtt cgagaattta agtctgaagc tggacctggc attcaaaacc ctgaaaggtc
    10921 aggggaaaat caccgagata aatattgctt cgacagttaa agaaatccgg cgcgccctca
    10981 ttgatgcgga cgtaaactac aaagttgcaa aggaagttac tgattctatt aaagagaaag
    11041 ctttaggaag agacgtactg atagcggttt ctcccggaca attaatgact aaaattgttt
    11101 acgaggaact aacagaattg atgggtggct ctaatgctgg cattaacctt accggagatc
    11161 ctgcggtaat attgattgca ggtttacaag gctccggtaa aactacgttc tccgcaaagc
    11221 ttgcgaatta tttgagtaag caaggccggc aggttttatt ggcggcctgt gatatttacc
    11281 ggcctgcggc catagatcaa ataaaagtct taggtgaaag cattggcata gaggtttacg
    11341 cggagcctga aaataaaaat gctctggaaa ttgcaaggaa cgcgataaag catggtaaaa
    11401 gtacggggaa gaaaacgatt attgtagaca ctgccggacg gcttgctgtc gatgaagcaa
    11461 tgatgaagga gatttcatct ttaaaagaaa tcttaaagcc ttccgagaca ttgtttgtag
    11521 tggattctat gaccggtcag gatgcggtga atacggcaaa gacttttaat gaacaaatta
    11581 attttgatgg tgtcgtcctg actaaacttg acggagatgc acggggaggt gccgcgcttt
    11641 ccattcgcag cgtggtagag aagcccatca agtttatttc taccggtgaa aagattgaag
    11701 cactggatct gtttcatcct gacaggatgg ccaacagaat tctgggaatg ggtgatgtgg
    11761 tttccctggt agagaaagcc cagcagacct ttgatgaaga ggagacccgc aggctgaaca
    11821 agaaattcag aaagaaccaa tttgattttg atgacttcct ttctcaactt cagcaaataa
    11881 agaagatggg taatataaaa gatatgatgg gcatgatacc gggagtaggg aaggcgatta
    11941 aagatataga tgtagatgat gaatccttca aacccattga agcaatcatc cgttccatga
    12001 ccctgcagga acgccaaaat cctgacctta ttgacggtaa tcgacggaag agaattgcca
    12061 atggtagtgg aaccagtgtt caacaggtaa ataacctgat gaaacaattt ggtgagatgc
    12121 gcaagatgat gaagcagatg aataagatgg gcggtgcgaa gaagcttgcg
//
LOCUS       DASWHX010000182         7026 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_578692, whole genome shotgun sequence.
ACCESSION   DASWHX010000182 DASWHX010000000
VERSION     DASWHX010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 7026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7026
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_578692"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..567)
                     /locus_tag="VGA21_11840"
     CDS             complement(<1..567)
                     /locus_tag="VGA21_11840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018622039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEX9651246.1"
                     /translation="MKTFLQLSVYFTALLFVTISVIAQESELTAIKGYPFPTGLTSSS
                     SGSKIAWAFDMQGKRNVFVAEGPDFSPRQVTNYDKDDGQEITSLSISADGNWLVFVRG
                     GDHGSNWGDDDPVNPEFRPFPDKVAIYAVPFEGGEPRMIAEADYPVISPKSDMIVFLR
                     NNQVWSASLLDTESKPESLFQTRGRVGSL"
     gene            765..3233
                     /locus_tag="VGA21_11845"
     CDS             765..3233
                     /locus_tag="VGA21_11845"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEX9651247.1"
                     /translation="MDIFTGFRSSSTMRILKVLSMTILVILMSFIPQEDFTLFLKEKL
                     TNYSLNYPQEKIYLHFDREEYFSGENIWFKVYLLNGITHRKDSLSLVVYIDFIGDSSK
                     VIQQKRIKIIKGVGYGNFTLDNTNDFQLLTVKAYTSWMRNYNPEFFFEKKLRIYGSAQ
                     PTFPEVKSNHDFQLLPEGGYLVSGVNNHLGFKATDKFGNGIEFTGRITEGVNTITEFA
                     PSHAGMGSVFFNPERGKEYYAEITFHDGTTRNIQIDNIQNDYLLQVDNSKIDFLNLKI
                     LLSPDFYKPSETAWVVIQAKGEVFFMAQARLSKEEIQIPVAKFTLPKGIVQCSIFDQD
                     GRPKGERLVFIDKPAENTIVVETNKTIYKPREVTEVKLTLSDNSGRPVQGDFSLTVFD
                     LVQVDPAGEFHFNIFDYLMLNSELSGYIESPGYYFDDENKDAAKDLDVLLMTQGWRRF
                     VWEKILNEEKPAYTHFMEQGFSITGILFNQRGKVLSDGSISLFLDGDKSGFMTSLVNE
                     KGVFGFYELDISDSTEIYIQAKDGKDRPDVTIKLDTLFVAPKFEITGVFFPDDEKLKT
                     PLEDLGFKRMQIREAFVLSGMDRVLDEVVIEGQRSKPRLARKQNQLYSVPDNRINLDD
                     LPAPGVYRNVFQMLQGRIAGVQITGDPQNPVINIRGTGQNGTTSIQNNVDPIILVDGV
                     QTDVSMAATIPMANVETVDVIKGLWSSMIYGGSGGMIAIYTKQGNITEARPLPGLTNF
                     TARGYSINKEYYKPNYAEENDLAEPDYRATLHWEPMIRIDSSGTFSLKYFNSDLENEI
                     QIQVEGVTDSGEPLIADYRYFIKK"
     gene            3347..4474
                     /locus_tag="VGA21_11850"
     CDS             3347..4474
                     /locus_tag="VGA21_11850"
                     /inference="COORDINATES: protein motif:HMM:NF012470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HEX9651248.1"
                     /translation="MNLTKVIFTATTFLAAISCTSITREANSETVPVPKNVILIIGDG
                     TGLSQISSAFFYKESAPNYGRFRHIGLIKTSSSRQKITDSAAGATAFASGVKTYNGAI
                     GVADDSTSVETIVEIASRRNIQTGVIATSSITHATPACFYAHVPNRGQAENIAADMVK
                     SEIDFLAGGGLRYFVRRRDSRNLLRELQEKGFGIDTVNLSSFTMLNSGAKLGYLLADD
                     AMSPVLEGRGNFLPNATDLGIQFLNKNNANFFLMVEGSQVDWGGHSNNADYLITELLD
                     MDDAIGKALDFAEKNGETLVIVTGDHETGGYTLSSTLKKTESGREYSDYNEITGTFST
                     DGHSGALIPVFAFGPGSEEFAGVYENTEIFHKIMKLTGWNK"
     gene            complement(4552..5775)
                     /locus_tag="VGA21_11855"
     CDS             complement(4552..5775)
                     /locus_tag="VGA21_11855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009283603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthetase B"
                     /protein_id="HEX9651249.1"
                     /translation="MRRWLTFLCCAFPYFSGASSILIPMDEFQQNHLKAYGIAYYSLQ
                     NDVPVDWLLNYRDGSFLMQYSKGIETECRVRGVSYEVISDPQTNSILAKVASPSVNMN
                     LVRMGRAPKIAVYSPRNDLIEDETDAVLLVLDYAEIPYDIIYDNEVLDDELVLYDWLH
                     LHHEDFTGQVDKFNWRWSNIMEGEMQKMNAQSLGYASVTDMKKDVALKIKSFLAGGGY
                     LFAMCSGAETFDLALSAEELEISPFTNGIVRYELDYTKTLAFTNFTLEDGYSRRFSDI
                     NVSRDWNRKLSYFSIFEFSAKWDVVPAILTQNHEHIIQEFSGLTTAFSKQTVKSSALI
                     LGENNANGNVRYLYGELGNGHWSYYSGHDPEKRNSRGRNGQPTDLSMFPNSPGYRLIL
                     NNVLFPSTKKKRQKT"
     gene            6436..6786
                     /locus_tag="VGA21_11860"
     CDS             6436..6786
                     /locus_tag="VGA21_11860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015266398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease domain-containing protein"
                     /protein_id="HEX9651250.1"
                     /translation="MKRAIELARELRRNQTQEEKKLWNILRNRKFLGKKFVRQHPVYY
                     QSISGSNFFLVDFYCHEKKLVVEIDGGYHDFQKDFDQNRDQILKAMGLIVLRIRNSEF
                     DDIANVLEKIGKYC"
BASE COUNT         2117 a         1431 c         1457 g         2021 t
ORIGIN      
        1 cagtgaacct accctgcccc gggtttgaaa gaggctttca ggtttagatt cggtgtccaa
       61 cagagaagct gaccacacct gattgtttcg caaaaaaact atcatgtcac tttttggaga
      121 aataaccgga taatcggctt ccgcaatcat cctgggttct ccaccttcaa atgggacagc
      181 ataaatagca actttatcag ggaatgggcg gaattccggg tttaccggat catcatctcc
      241 ccaatttgat ccatgatctc cacctctgac aaatacgagc caatttccgt ctgctgaaat
      301 tgagaggctg gtaatttcct ggccatcatc tttatcataa ttagtaactt gtctgggtga
      361 aaagtcgggt ccttcggcaa caaagacatt ccgcttcccc tgcatatcaa atgcccaggc
      421 aattttagaa cctgacgatg acgaggttaa gccggtagga aaagggtatc cctttatggc
      481 cgtcagctca gattcctggg caatgactga aatagttaca aaaagtaaag cagtgaaata
      541 cacggagagt tgtaaaaaag ttttcatgat gcgttggatt aaatgattta agaaaattta
      601 aggatattaa tttgaaaaca aaacttaatt tttacaacag gaaaaaaatg ttcatatcac
      661 cttatctaag tctgttcgtt gaagatatct ctcctttcat tgaattcttt tcagggaatc
      721 tttctatttt atatctttat aacataatac caaattcaaa atgattggat atttttacag
      781 gcttcagaag tagttctacc atgagaattc taaaagtttt atcaatgaca attttggtaa
      841 tactgatgtc tttcattcca caggaagatt ttactctatt cctaaaggaa aagcttacga
      901 actattcgct gaattatccg caggaaaaaa tttaccttca ttttgacagg gaagaatatt
      961 tttcaggcga aaatatctgg ttcaaggttt atttgctgaa tggaattaca cacaggaaag
     1021 attcattgtc tctggtcgtt tatatagatt tcatcggtga cagttcaaaa gtaattcaac
     1081 aaaaaagaat aaaaataata aaaggcgtgg ggtacggtaa ttttactctt gacaacacga
     1141 atgacttcca gctgctgaca gtgaaggctt atacttcctg gatgcgaaat tataatcctg
     1201 aatttttctt cgagaaaaaa ttacggattt atggttctgc tcaaccaaca tttccggaag
     1261 ttaaatctaa ccatgacttc caattattac ctgagggtgg ttaccttgtg tcaggagtta
     1321 ataatcacct gggattcaaa gctaccgata aatttgggaa tgggattgaa ttcactggtc
     1381 gaataacaga gggggttaat accattactg agtttgctcc atcgcatgca ggaatgggat
     1441 cagttttttt taatcctgaa agaggaaagg aatattatgc agaaataacc ttccatgacg
     1501 gaactactag aaacatacaa atcgataata ttcagaatga ttatttactt caggttgata
     1561 actctaaaat tgattttctg aatttaaaaa ttcttctctc ccccgacttt tataaacctt
     1621 cagaaactgc ctgggtggtt attcaggcaa agggagaagt cttcttcatg gcacaagcca
     1681 ggctatcaaa agaggagatt cagatcccgg tagcaaagtt tactttgccg aaagggattg
     1741 tgcaatgctc aattttcgat caggatggaa ggccaaaagg ggaacgcctg gtgtttattg
     1801 ataagccggc cgagaatacg attgtggttg aaacaaataa aacaatatac aaacccaggg
     1861 aagtgactga agtaaaactc accttatccg ataactccgg aagacctgtt caaggtgatt
     1921 tttccttaac tgtatttgat ttggttcagg tagatcctgc tggtgaattt cattttaaca
     1981 tttttgatta cctgatgctc aattcagagc tttccggata tattgagagt ccaggatatt
     2041 atttcgatga tgaaaataaa gatgcggcca aagatctgga tgtcttactt atgacccagg
     2101 gatggcgccg gtttgtttgg gaaaaaatat tgaatgaaga aaaacccgcc tacacacatt
     2161 ttatggagca aggattcagt atcaccggaa tcttattcaa tcagagaggt aaagttttga
     2221 gcgatggtag tatttctctc tttctggatg gtgacaaatc gggatttatg acttcactgg
     2281 taaatgaaaa aggtgttttt ggcttctatg aacttgacat atcagattcc actgagattt
     2341 atatccaggc aaaggatggt aaagaccgac ctgatgtgac cattaaatta gatactcttt
     2401 ttgtagcgcc aaaatttgaa atcaccggcg tcttttttcc ggacgatgaa aaattaaaaa
     2461 ctcctcttga agacctggga tttaagcgaa tgcaaatccg cgaagcgttc gtactttccg
     2521 gcatggatag agttctggat gaagtggtta tcgaggggca gcgatccaaa cccaggctgg
     2581 ccagaaagca aaatcaactc tattcggttc cggataaccg gataaacctt gatgatttgc
     2641 cggcacccgg cgtatataga aatgtgtttc agatgttgca gggccggatt gccggggttc
     2701 agataacagg cgatccccag aatcctgtta ttaatatccg gggaacgggc cagaatggta
     2761 caacctcaat tcaaaataat gttgacccaa tcatactggt tgatggtgtt caaacggatg
     2821 tgagcatggc ggcaacaatt ccaatggcca atgtggaaac tgtggacgtt attaaaggac
     2881 tctggtcctc catgatatat ggcggctccg gtggtatgat tgctatttat actaagcagg
     2941 gtaacattac tgaggccaga cctttacccg gtttaaccaa ctttacggcc aggggatatt
     3001 caattaacaa agaatattac aagcccaatt atgctgaaga aaatgatctt gctgaaccgg
     3061 attatagagc tactcttcac tgggagccaa tgatcaggat tgattcctcc ggaacttttt
     3121 ctctgaaata ttttaacagc gatttggaaa acgagattca aattcaggta gaaggagtaa
     3181 cagactccgg agaaccgcta attgctgatt atcgttactt tattaaaaaa tgaatttccc
     3241 aaaccacatt ccggaaatca gtatatcaaa attagaagaa ttctctggat ttacttttat
     3301 gagccgtatc taagctcgat tttagcatat tgaaaaacca tagtgtatga acttaaccaa
     3361 agtaattttt accgccacta catttttagc cgcaatatcc tgcacttcaa taaccaggga
     3421 agccaactct gaaactgttc cggttccgaa aaatgttatt ttgataattg gagatggcac
     3481 cgggctttca caaatttcct ccgctttttt ttataaggag tcagcaccaa attatggtcg
     3541 gttcaggcat attggcttaa ttaaaacttc aagcagcagg caaaaaatta cagattcagc
     3601 tgccggcgct acagcgtttg caagcggagt gaaaacctat aatggtgcca tcggtgttgc
     3661 tgatgattca acttctgttg aaacgatagt agaaatagcc tcaagaagaa atattcaaac
     3721 cggggtcatt gccaccagtt caattaccca tgctactcca gcttgttttt acgcacacgt
     3781 gccaaatcga ggacaagctg aaaatattgc ggcggacatg gttaaatcag aaattgattt
     3841 cctagcggga ggtggactga gatattttgt gcgtagaaga gattccagaa accttttgcg
     3901 ggaattgcag gaaaagggat ttgggattga tactgtcaat ttaagttctt tcacgatgct
     3961 taattccggg gcaaaacttg gatacctcct tgccgatgat gcaatgtctc ctgtattgga
     4021 aggtcgaggc aattttttgc caaatgctac cgatttgggg atccagttct taaataaaaa
     4081 taacgcgaat ttcttcttaa tggtagaagg ttcccaggtt gactggggag gacattctaa
     4141 taacgctgac tatttaatta ccgaactcct tgatatggat gatgccattg gcaaagcact
     4201 cgattttgca gaaaaaaatg gagaaactct agtcatagtt accggagatc atgaaactgg
     4261 cgggtatacg ctttcatcca ctctaaagaa aactgaaagt ggcagggaat attcagatta
     4321 caatgaaatt accggaacct tttctacgga tggccattct ggcgcgttga tccctgtttt
     4381 tgcttttgga cctggatcag aagaatttgc gggagtgtat gaaaatacag agatctttca
     4441 taagatcatg aagttgaccg gttggaataa ataagacacc tttggcagga aaatactcta
     4501 tgatataact atattgattc ccggagaaat ttactcgcaa tctaaattct ttcaggtctt
     4561 ctgacgtttc ttctttgtag aaggaaataa tacattattc agaattagtc tgtatcccgg
     4621 cgaattggga aacatactca aatcggtagg ttggccattc cttcctcttg aatttctttt
     4681 ttccggatca tgcccactat agtagctcca atgtccattg ccaagttctc cgtataaata
     4741 gcgaacattt ccgtttgcat tattttctcc caaaataagt gcggaagact ttacggtttg
     4801 tttgctgaat gcggtagtca atccggaaaa ttcctgaata atatgttcat gattctgggt
     4861 aaggattgcc gggaccacat cccatttagc agagaattca aaaatagaaa aatagctaag
     4921 tttccggttc cagtccctgc taacattaat atcagaaaat cttctgctat acccatcttc
     4981 aagagtaaaa tttgtaaaag ccagggtttt ggtataatcg agttcatacc ttacaatccc
     5041 atttgtaaat ggactaattt ccaattcctc cgctgaaagc gccaaatcaa acgtctccgc
     5101 tccggaacac atggcaaata gatacccgcc acccgccaga aatgatttta ttttcaaagc
     5161 aacatctttc ttcatatccg taactgaggc atatcccaag ctttgagcat tcattttttg
     5221 catctcccct tccataatgt tagaccagcg ccagttaaat ttatccactt gtccggtaaa
     5281 atcctcatga tgcaaatgca accagtcgta aagaaccagc tcatcatcca aaacttcatt
     5341 atcataaata atatcataag gaatttctgc atagtccaga accagcaata cggcatcggt
     5401 ttcatcttca atcaaatcat ttctgggaga ataaacggca atttttggag ctcttcccat
     5461 ccggaccaag ttcatattta cagaaggact tgctactttc gcaagaatcg aattggtctg
     5521 cggatcagaa attacttcgt aggaaactcc ccttactctg cattccgttt caattccttt
     5581 cgagtattgc atcagaaaac tgccatcacg gtagttcagc agccagtcga ccggcacatc
     5641 attttgcagc gaataatatg cgatcccgta cgcctttagg tgattttgct gaaattcatc
     5701 catggggata aggattgaag atgcaccaga aaaataagga aaagcacaac aaagaaacgt
     5761 caaccatctt ctcatgggca aagcttttaa cactaactaa atttcctcaa ttttcaaagg
     5821 aaaagcaatc tccaagcaga ttttaacctt gaacccttcg ggcggggatg agtaagtttg
     5881 atttctaact ctcccgccct tcgaacacac cgctctatat taagaaagtg cccgcgggtg
     5941 agcaagtttt cttactcccc acccttcggg caccccgctt ttttaaggga gggaccgctc
     6001 tattaagaaa gtagccgggg gtgagcaagt tatttctaac tctcccagcc ttcgaacaca
     6061 ccgctctttt aagagagggg tcgggggtga gcaagttttc taactctccc gcccttcggg
     6121 cacaccgctc tattaagaaa gtggccgggg gtgagtaagt tttctaactc tcccgccctt
     6181 cgggcaccct ctcttttaag agaggggccg ggggtgagca agttttctaa ctctcccgcc
     6241 cttcgaacac accgctctat taagaaagtg cccgggggtg agcaagtttt ctaactctcc
     6301 cgcccttcgg gcacaccgtt ctattaagaa agtgcccggg ggtgagtaag ttgatttcta
     6361 actctcctgc ccttcgggca ccctctcttt taagagaggg gccgggggtg agtcaaaaat
     6421 acctaacttt aaaatgtgaa aagagccatt gaattagcac gggagttaag aaggaatcaa
     6481 actcaagaag agaaaaaatt gtggaatatt ctcagaaaca gaaaattcct tgggaagaag
     6541 tttgtgagac agcaccccgt ctattatcaa tcgatttcag gatccaattt ctttctagtt
     6601 gatttctatt gtcatgaaaa aaaacttgta gtagaaattg atggtggata tcatgatttt
     6661 caaaaagatt tcgatcaaaa tcgcgatcaa atactaaaag caatgggatt gatcgtatta
     6721 agaatcagga attcggagtt tgatgacatt gctaatgttc tggaaaaaat tgggaaatat
     6781 tgctaactct ccccgccctt cgggcacccc tctcttttta gagaggggct gggggtgagt
     6841 accttgattt ctaactctcc ctcccttcga acgtgagtaa gtttgatttc taactctccc
     6901 agccttcgaa cacaccgctc ttttaagaga ggggtcgggg gtgagcaagt tttctaactc
     6961 tcccgccctt cgggcacacc gctctattaa gaaagtggcc gggggtgagt aacaccaagg
     7021 ctacaa
//
LOCUS       DASWHX010000183        62754 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_2063920, whole genome shotgun sequence.
ACCESSION   DASWHX010000183 DASWHX010000000
VERSION     DASWHX010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 62754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62754
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2063920"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..616
                     /locus_tag="VGA21_11865"
     CDS             <1..616
                     /locus_tag="VGA21_11865"
                     /inference="COORDINATES: protein motif:HMM:NF036996.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEX9651251.1"
                     /translation="LASAKAGRNPQVPEVCIYFNYLLFRGNRVKKVESNHFDAFRSEN
                     YPALAQSGVRIEYNVNFIRKVQQGRLKANVKIDTNVSLIKLYPGINRLIVEAMLRTKK
                     LKGVVLETFGSGNVPTEKWLISSLQKAIKRGVIVLNVSQCHGGKVEQGKYAASKVLKE
                     IGVLSGSDITTEAALTKMMVIFGKGGSLNNQKKQLLKPICGEMT"
     gene            695..785
                     /locus_tag="VGA21_11870"
     tRNA            695..785
                     /locus_tag="VGA21_11870"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:729..731,aa:Ser,seq:gga)
     gene            854..1678
                     /locus_tag="VGA21_11875"
     CDS             854..1678
                     /locus_tag="VGA21_11875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009197346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEX9651252.1"
                     /translation="MKKLITKLAFIGILMIGFTNAGFSQDDTTSVADQDTAEVQDQPE
                     EQPAVETQPVYEEPADESGSTFHQKVKEKFIEGGWQFMGIVLLCLIIGLAISIERIIT
                     LNLATTNTKKLLQNVEEALGRGGVDAAKEVTKNTRGPVASIFTQGMLRMSEGIEMVEK
                     SIIAYGSVEMGRLEKGLVWVSLFISLAPMLGFMGTVIGMVEAFDAIEAAGDISPAIVA
                     NGIKIALLTTVAGLIVGVILQLFYNYLVSKIDGLVNEMEDASISLVDLLVKHNLSK"
     gene            1699..2025
                     /locus_tag="VGA21_11880"
     CDS             1699..2025
                     /locus_tag="VGA21_11880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651253.1"
                     /translation="MEQIVNIGLIAAYILVIGGGLSAIILPLINALNNPKSLMYAGIG
                     IAGLLFLFLIGYLIAGNEVTANYIKYGVDAGTSKIVGGALICMYILTLFAIVGILYTE
                     FSKLIK"
     gene            2025..2615
                     /locus_tag="VGA21_11885"
     CDS             2025..2615
                     /locus_tag="VGA21_11885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009185401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEX9651254.1"
                     /translation="MARSKRSQPEINSSSMADIAFLLLIFFLVTTTIANDKGLTIQLP
                     PKPEDMDIIDIKIKERNLFKVYVNSADRILVEGEILEDISDIRQKVIDHVLNNGRDPQ
                     KSESPKDAIVSYKTDRGTSYEVFIKILDELQGAYYDMYAERAGVTNKQWREIASDHKE
                     PEKAKLYDIGRGLLPNGDVTFPMNISIAEPSKIGGN"
     gene            2617..3081
                     /locus_tag="VGA21_11890"
     CDS             2617..3081
                     /locus_tag="VGA21_11890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017732908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEX9651255.1"
                     /translation="MSKFRKKAKTKQDIPTSALPDIIFMLLFFFMVTTVLRETTINVQ
                     QRIPQAIELRKLQRKSLVSYLYIGKPIKTEVFGDEPKIQANDVFIDTKDIVMWVTQEK
                     DKLDEVERDQLTVSLKVDMEAKMGLISDVQLELRNANARKLMYSAPPRRAEN"
     gene            3141..4451
                     /locus_tag="VGA21_11895"
     CDS             3141..4451
                     /locus_tag="VGA21_11895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008198965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9651256.1"
                     /translation="MKTVVLNNQKVINAWCMFDWANSVYSLVIISAIFPVFYNSATEA
                     AFGGETINFFGYEIVNSVLYSYALSFSFLIVAAILPLLSGIADYGGLKKGFMKFFTMM
                     GGISCIALYFFTGKNVELGIIASIVASVGFSGSLVFYNSYLPEITTEDKFDRVSARGF
                     SFGYIGGVVLLVINLLMIEFYDSFGLPDSSSAARFSFLTVGLWWIGFSQITFYYLPKN
                     IHGKKSAGHFLISGYREIVTVWKSMAHLPNLKRFLFSFFFYNMGVQTVMYLAVLFGTK
                     ELNLADSKLIISVLIIQLVAIMGSYLFAIVSEKKGNKFSLTFMIIVWVMICIAAYFTA
                     TEYHFYALAFFVGMVMGGIQSLSRATYSKLIPENTQDTASYFSFYDVTYNISIVLGTF
                     AYGAIEQLTGSMRNSTLAIGVFFLIGIAILSKVNMPRSNQNLTV"
     gene            complement(4428..6215)
                     /locus_tag="VGA21_11900"
     CDS             complement(4428..6215)
                     /locus_tag="VGA21_11900"
                     /inference="COORDINATES: protein motif:HMM:NF015557.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEX9651257.1"
                     /translation="MIPYEVQAYFFLILIAVTLVLIFKEVVRPSVGFLLAVVVLLIFG
                     IILPEDLLAGFSNDKIASIMLLILITSAIRKNFRLELLFDNLFGGTKKYKVFLIRMMG
                     QVAILSSFINNTPVVALMSPYVVEWGKKNKISSSKLLIPLSYAAIMGGMLTIIGTSTT
                     LLLNGFLDEFSIAGLNPFDLLFVGAGSTLVGVLFIAIIAPFLLPDRKDLVEEFEKNTR
                     EYIIETHIAGDSHLVGKSVKEGGLRNLKGVYLVEIIRNKEVIYPVSPDNKIEANDKLF
                     FAGSTEQIVELVNSDLGLILPPKITDIQSVESQDIVECVLAENSTLTGKTIKQSDFRH
                     RYDAAVVAIHRAGERLTGRIGDLKLRPGDLLLLYAGHDFSERVDLYRDLYVISSIPKS
                     VGSNRTRFIALSLIVVSIITLLTVGNFSLFSSLLVILAIMVGFKLITLREIKRELDLD
                     LIGILVFSLVLGEGMLKTGTGIFLSEGLMGLLGGFGPLVLLSGIMLFTMMLTSFINNA
                     AAVSIAFPVTYSLIQTLNMDGSPFYLGIAFAASGSFMTPFGYQTNLIIFGPGGYKISD
                     FIKIGLPITIVYFISCLLLILVLYREVLV"
     gene            complement(6221..6991)
                     /locus_tag="VGA21_11905"
     CDS             complement(6221..6991)
                     /locus_tag="VGA21_11905"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009581942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HEX9651258.1"
                     /translation="MKTKKEIVTDWLPRYTGMELDQFGKYILLTNFINYVELFSSKFS
                     VEIVGKEKPMQTATFDDITILNFGMGSPMAATVMDLLSSIKPRAVLFLGKCGGLKKKT
                     QIGDLILPIAAIRGEGTSSDYFPPEVPALPSFSLQRAVSSMIKKHQMDYWTGTIYTTN
                     RRIWEHDTSFKKYLRKIRVMGIDMESATIFITGFKNEIPTGALLLVSDNPMVPHGVKT
                     SKSDHSVTTSFVGKHLQIGIDSLQELINSGDSVKHLRY"
     gene            7018..7470
                     /locus_tag="VGA21_11910"
     CDS             7018..7470
                     /locus_tag="VGA21_11910"
                     /inference="COORDINATES: protein motif:HMM:NF024976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HEX9651259.1"
                     /translation="MEILNFPEFRFDFKKQEDKILIYDIIRKKHVILTPEEWVRQHLV
                     HFLIFKKFFPKSLMKVESGLNYHKKQKRADLIIYNDSGVPFIIAECKSPKSIINQNAL
                     FQIGVYQKSISPKYLIVTNGITHFMWRKNNDLSRYDQIPEIPEWDYIS"
     gene            complement(7467..8579)
                     /gene="ald"
                     /locus_tag="VGA21_11915"
     CDS             complement(7467..8579)
                     /gene="ald"
                     /locus_tag="VGA21_11915"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009581940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HEX9651260.1"
                     /translation="MIIGVPKEIKNNENRVSVTPGGVKELTKHGHKLYVQSSAGEGSG
                     FPDEDYRNAGAEILNDIESTYTKADMIIKVKEPIEQEYSLIKNDLLLFTYFHFASYEP
                     LTKAMMSSKAVCLAYETVEKSDGSLPLLIPMSEVAGRMAIQEGAKYLEKPLKGRGVLL
                     GGVPGVKPAKVLILGGGVVGTNAAKMAAGLGAEVTIMDVNLPRLRYLDDIMPANVSTM
                     MSNEYNIRELIQTADLIVGAVLIHGAKAPSLITKDMLKTMRPGTVVVDVAVDQGGSIE
                     TCKPTTHENPTYIIDGVVHYCVANMPGAVPYTSTLALTNATLPYAIDLANKGWKKACT
                     DRHDLKLGLNIINGEIVYKAVGETFNLPFAPVDKFF"
     gene            8758..9105
                     /locus_tag="VGA21_11920"
     CDS             8758..9105
                     /locus_tag="VGA21_11920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651261.1"
                     /translation="MVSKIDEFEIPDYEDGSKLKIIVNSCSELGNSAKPGLQIFFMGS
                     FVNYEPKIAEKWRYELNKASSEELLLQDKSWIPNEENFIKVFFVNSTPAMAKIMIKTR
                     SSEVISKQYELPF"
     gene            9232..11628
                     /locus_tag="VGA21_11925"
     CDS             9232..11628
                     /locus_tag="VGA21_11925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009055099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX9651262.1"
                     /translation="MEKSKTKRTLPGKAEVLADYRLAVASREASLIGRKEVFMGKAKF
                     GIFGDGKEVAQLAMAKVFQNGDIRSGYYRDQTFMFAIGELTIKQYFAQLYADPSVVAD
                     PSSAGRSMNGHFATRMLDENGKWKNQTNYKNSSADVSPTASQMPRLLGLAYASKLYRN
                     NKELGRLKDFSKSGNEVAFGTIGNASTSEGMFFETMNAAGVLQVPMLISIWDDDYGIS
                     VPKEFHTIRGSISAALEGFGRTDNMPGFEILKVKGWDYEGLIETYQTAEKVCREEHIP
                     VMIHVTEMTQPQGHSTSGSHERYKSKERLKWEKDFDCISKLKEFILENKIESPEQLNE
                     IEKEAKNEAKVARNEAWTDFMTGINAEQKEAVSLLKKSATQNQEINSLIDELEKTINP
                     IRADAIRVVKKAIRILRTEHNDERNNLVKWILKQEKKNYERFSSHLYSESAESALTIV
                     KVDPVYDSEPVLVDGREILQANFDKIFEMDPRVLAIGEDVGKIGDVNQGMAGLQEKYG
                     ELRLTDTSIRECTIIGQGIGCALRGLKPIAEIQYLDYLLYAIQILSDDLATLQYRTKG
                     GQKAPMIIRTRGHRLEGVWHSGSPMGMIINSLRGIYVLVPRNMTQAAGFYNTILASDD
                     PALIVEPLNGYRLKEKLPVNPGEFKTPLGIPEILREGSDVTVVTYGSMCRIVMDAASQ
                     LSDTGIECEVIDVQSLLPFDINHSIVESLKKTNRIIFMDEDVEGGTTAYMMQQVLEVQ
                     GGYQFLDSLPATLTSRPHRPAYASDGDYFSKPNSEDVFDKVYSMIAEANPSKFPDLYN
                     "
     gene            complement(11625..12341)
                     /locus_tag="VGA21_11930"
     CDS             complement(11625..12341)
                     /locus_tag="VGA21_11930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651263.1"
                     /translation="MMVRISIFILLVILAGCTSSTEFPTVPKIEFVKAEYWDMGLQNQ
                     DSINITIHFQDQQGDLGIFSSENIGPFAAVGYFLDGDQELVTYGHRTRPGFESLPPYE
                     EPYKCTNYIINPTINNITVMDTVYFVANENHYNFLIDFLIKNSDGTFTRFPWESYLGY
                     PDCGQSFNGRFEPADLGIDLRKESPIEGNLTYRMLSLGFPVLLRNKVFKFRIRIKDRG
                     FHTSNVVETPEYTLQDIKEN"
     gene            12388..13365
                     /locus_tag="VGA21_11935"
     CDS             12388..13365
                     /locus_tag="VGA21_11935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl transferase"
                     /protein_id="HEX9651264.1"
                     /translation="MFRIDSKTLQSLSSITFEKLAFDIFKYQSKNNPVYAKYLKLVEI
                     KPGNITGLNQIPFLPIELFKTNIVKTGNWKEETIFQSTGTSGQPSIHYVRDLSFYQEL
                     AAFNFQRFYGKLNDYDIYALLPGYVGSTNNSLIAMISHFIEKSGSLKSGFFLTSHGKL
                     VRLLSSNSEGKKRLLIGVSHALLDFALRNPVKLKNTIIMETGGMKGRKKEITRKELHD
                     ILKSKLDVGKIHSEYGMAELTSQGYSIGDGYFEFPNWMRIIIRDITNPFQRVTNGKAG
                     GINVIDLSNYQTCSFIETKDLGRMKNEKKVEILGRFDNSDVRGCNLLVR"
     gene            complement(13569..14720)
                     /locus_tag="VGA21_11940"
     CDS             complement(13569..14720)
                     /locus_tag="VGA21_11940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018475671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX9651265.1"
                     /translation="MAKILILDDEKSIRDTLREILEYEKFEVDEAKDGQEGLDKLIRN
                     GYDAVLCDIKMPHLDGMEVLQKAKEFNLDTQFIMVSAHGTIETAVEATKLGAFDFVQK
                     PPDLNRLLLTIRNALDKSTLVSQTKVLKRKINKAYDIVGESESIQEVKETIEKVAPTD
                     ARVLITGPNGSGKELVARWLHEKSQRSEGPLIEVNCAAIPSELIESELFGHEKGSFTS
                     AVKQRIGKFEQAHGGSLFLDEIGDMSLSAQAKVLRALQENRITRVGGDKDIKVDVRVI
                     AATNQDLKQLIEEKKFREDLYHRLCVIIIPVPGLTERIEDIPLLVQHFLVEITAEYGI
                     KPKKITDNALKALKSMKWTGNIRELRNVTERLIIMCGDTIDEPDIKKYA"
     gene            complement(14735..15112)
                     /gene="rnpB"
                     /locus_tag="VGA21_11945"
     ncRNA           complement(14735..15112)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VGA21_11945"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(15168..16064)
                     /locus_tag="VGA21_11950"
     CDS             complement(15168..16064)
                     /locus_tag="VGA21_11950"
                     /inference="COORDINATES: protein motif:HMM:NF021554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2279 domain-containing protein"
                     /protein_id="HEX9651266.1"
                     /translation="MTRLTRILCGAFLLILIIPFPGSGSGHGDSLNKKRTTALLVGLP
                     VLHGSSIVLFNELWYQNQPRENFHFFDDGKEWNQVDKAGHLYSGFHFNAIASRLYQWC
                     GFNDMKSDLYGSATSFAFMSTIEILDGYSSEFGASGWDVLANAGGIGLYLGQKLVWDE
                     IRIHPKFSFSPTGFADTRPQVLGGNFMEQLFKDYNGQTYWLSFDLNKFFENKFRFPKW
                     LNIATGYGSTNMIYARKSENYNAGYNSYRQYYLSLDIDLSHIHTRSAFLNSILYFINM
                     IHLPSPGLEFNKNGVKVRYLTF"
     gene            complement(16048..20562)
                     /locus_tag="VGA21_11955"
     CDS             complement(16048..20562)
                     /locus_tag="VGA21_11955"
                     /inference="COORDINATES: protein motif:HMM:NF016260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HEX9651267.1"
                     /translation="MFWVLGTPLILLVGVASALQIPYVQTKVVQRLGEMLTKTTGFIT
                     TVDYVSINWFDKIAVEGISVKDLQDSSMFKADEAVIDFTISNITSTYEIVIDEIVLFN
                     ADFKMFRNPESARFNIQEYIQTIVAHYKKEGASTRSFVIRTVKLFSTTYTLWDASKPV
                     NENYFDINHFTINNVKSQVDNLTILKDTITMDIKSLSGFNIQNKTEIHSITGLLSIHP
                     SRLKLTELQAAIGKSYISDEIIFNFDGYQSLSDFVPNVNIKTTLKSCVIASEELSLFV
                     PAFSGYSEVFTLSGSVNGKIGDLKIKDLDLALGNQSLIKGDFILNGLPELNDFYFDAT
                     ISKSRILISDLKDYVPENVYQRISKLNDLFFFGSLNGNIHDFLARGNFESDLGQIDCD
                     LNLKIPRKDSKEIISYKGNLELTDFDIGEFFQDNILQKVSLKGSIDGRGVTLSTAAFK
                     LDGVISEIGINGYNYSDIVTNANFAKEFFDGNIRISDPNLRFNATGSIDLREKKNEIK
                     IHGRIDTLFFEPVGLLPSDLFLRTEFDVNVTGLQVDSLQGRIITKNTMLINPEGQLNL
                     DSLNLFSQRNSAFRLLKVNASDFSVDLQGDFNYTSALRDITALVKEFQLSFKNQSDSL
                     SQYYRFKSEQISKNDYYVDYFINLKDINPLLQFLVPEISVSKNSQIQGRFSSGFTSIF
                     SLSTSIDTVNYQKSSFFDNEIDLSASKISDSTQILAMLYVYSKNQLINELTRTKDMTL
                     DCVWSNNEMDFNATIAHENNLNYADIFGTVNFLKDSTTIHIKPSDILALNENWRIDND
                     NLIASSGREISVKDLRFFNKNQSIELNGDISEKADKMLTVIIDSVRMENINPLINRNL
                     TGIINGGVRLSDLYHHPLIDSRVEILSMRINDFLVGNIQGRSEWIPQSELFEIDFKVD
                     REEKDIIDVTGLFEPFESENSLNLKAKFSEANLKIIEPFIETIFSNISGTISGEFSIT
                     GTPLFPYINGAGIVNDGSLMVNYLNTNYNLAGDIAFTESKIRFQNIQVTDIDNNNSFL
                     SGEFTHNRFRNFSLNLSGNLDNFQVLNTTSKDNDLFYGNAKVTGKVSFNGPLSKLKIS
                     ANARTNRGTRLFIPLGDTESIEARDYITFVNFSSGEIDSIQGEEKTVNLKGLLLDFDL
                     EITPDAYAEMIFDIQSGDIIRGRGNGKIKLQIDTQGDFAMIGDYEFISGGYNFTLYNI
                     INKEFSILPGSSISWAGDPYRGILDLQASYMQQTSLGPLMDTTVYNHSPESNRRYPTE
                     VLLNLAGPLLSPNIAFDIKVTSYPDIITLDNGQSFSLASMVSSFENRIASDEQEMKRQ
                     VFSLLILRRFSEQEGLLSGSNYIVNSVSEFLSNQLSYWITQLDENLDIDVDLGQLDQE
                     GFNAFQLRLSYTFLEGRLRVTRDGGFTDAENKADFASIAGDWTVEYSLTPEGKFRVKM
                     FNRTNTNQALTTFNQTSNFTAGVSLIHTQSFNEVRDIFNASRKRRLKEIESRDPADTS
                     ARKNEDDPLN"
     gene            20614..21639
                     /gene="tsaD"
                     /locus_tag="VGA21_11960"
     CDS             20614..21639
                     /gene="tsaD"
                     /locus_tag="VGA21_11960"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009185219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEX9651268.1"
                     /translation="MHPAILAIESSCDETAASIIVDGKVLNNIVATQFVHKNYGGVVP
                     ELASREHQKNIVPVVDEALKKAAINISDLDAVAFTQGPGLLGSLLVGASFAKSIAMVN
                     SIPLISVHHMQAHILSHFIDKPHPAFPFLCLTISGGHTQIILVIDYLIMKVLGETRDD
                     AVGEAFDKGAKILGLPYPGGPLIDKYSKKGDENFVDFPVTELAGLDFSFSGIKTSLLY
                     YLRDQQKKNRNFINDHLNDICASYQKALVKMLLIKLEQASKETGIKELAIAGGVSVNS
                     FLRNSLDEKSKEMNWNVHYPKFEYCTDNAAMVAMTAHYKYINNQFEKADVKVDPRLKF
                     PENEINF"
     gene            21623..22018
                     /locus_tag="VGA21_11965"
     CDS             21623..22018
                     /locus_tag="VGA21_11965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009581518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651269.1"
                     /translation="MKSIFKPGDSRKLEIKVSRDDLAKFNGIVVHEVCSTFALARNIE
                     WATRQYVLEITDDDEEGIGTSISIDHVGPAFEGEVLEIESEVKDFNGHELICAYQVKV
                     KNRIIATGTTGQKILKKERIEKIFSSLPG"
     gene            22018..22482
                     /gene="smpB"
                     /locus_tag="VGA21_11970"
     CDS             22018..22482
                     /gene="smpB"
                     /locus_tag="VGA21_11970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010853795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEX9651270.1"
                     /translation="MAKEKRFSGTVNVKNKRASFEYEFLEKFIAGIALTGTEIKSIRE
                     GKVYLQDGFCVFHNHELYLHQVTISPYKQGGHYNHIGDRERKLLLKKKELKKLKSKLE
                     EKGLSIIPTRLFINERGLAKIEIALGRGKKLYDKRESIKEKDLKRDMERLKL"
     gene            22485..23279
                     /locus_tag="VGA21_11975"
     CDS             22485..23279
                     /locus_tag="VGA21_11975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009581516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEX9651271.1"
                     /translation="MEFLERGICRLGIVPVRSAPGDTCEMVNQLLFGEHYTVLELSDS
                     KEWIRIKAYFDEHEGWINSRQHHRITEDYFNQINNSDYKISLDISSSILYRKNTIHIV
                     MGSILPISTNELFKMEEKLAFNGESKSLGQKREFEFLKQVAGKYLNSPYLWGGKSPFG
                     IDCSGFVQQVFKVCGYKLPRDSHRQAEIGTKIDELPDVLPGDIAFFKNSKGKIAHVGI
                     ILEDGNIIHASGQVRIDKLTEHGIVDEKLKQLTHKLFSIKRILKTL"
     gene            23276..23785
                     /locus_tag="VGA21_11980"
     CDS             23276..23785
                     /locus_tag="VGA21_11980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015028274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEX9651272.1"
                     /translation="MNKRVLVLNLDHSPISVCTVQRAFLLVYLNKAQSLMHTKGFYLR
                     SVSMSYPMPAVIKLNNYVQIPYMGVELTRQNIFKRDSFTCQYCGHQKGLTLDHVVPRS
                     KGGKTSWTNLVTACKRCNTQKGDYTPEEAGLNLINRPFRPSYIMFLRDFSGFVQEEWE
                     PYLATHMAS"
     gene            complement(23778..25130)
                     /locus_tag="VGA21_11985"
     CDS             complement(23778..25130)
                     /locus_tag="VGA21_11985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015028825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9651273.1"
                     /translation="MGFNRFRIGVFIRVILLGLCVSGVALLINDPSKIVSAIILIGIS
                     ILILVEIFRYVEKTNRKLTRFLESVRYSDFISGFTSDNKLGQSFADLNQAFNAVLEAF
                     RVARGEKEEHWQYLNTVVQHVTTGLLSFDEEGNVELINARTKHFFGSPQLRNIEELKD
                     KQPELHRMLVNLKPGINRMLRLNSNVHLAVHATELKLRGKIYKLVSLQNIQSELQQKE
                     VEAWQNLTRVLRHEIMNSITPISSLTSTLNEILVEDLVRRNNKIEILEETIDDLEEGL
                     NTIKSRSTGLIRFVEAYRDYTTIPTPKLKKILLHELLEQIASLHRAEIRKGNIELTME
                     VKPEDLFIFADEELIQMVLINLVKNSIEAAGKVKNPRISLKGSVDFELKATIEITDNG
                     EGIIPEALERIFVPFYTTKKAGSGIGLALSRQIMQLHNGTISVESTPGSRTTFTLRFN
                     "
     gene            complement(25120..26511)
                     /locus_tag="VGA21_11990"
     CDS             complement(25120..26511)
                     /locus_tag="VGA21_11990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017733554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX9651274.1"
                     /translation="MNNTQNKSGKILIVDDNEDLLKAAKIFLKRHFLQVDLEKSPEAL
                     PTVLNNESYDIILLDMNFTKDVSSGKEGYYWLERILEIDPSAVVVLITAYGDVDTAVQ
                     AIKAGATDFVLKPWDNQKLLATLHSAMKLRESKLEIKDLRTKQKHLNEDLNNKYLEII
                     GQSLVMEKIYETIDRVAKTDANVLILGENGTGKELVARAIHRNSHRANEVFINVDLGS
                     ITETLFESELFGHMKGSFTDAKEDRAGRFEVASGGTLFLDEIGNLSLPLQAKLLTAIQ
                     NRTITRVGSNKIIPIDIRLVCATNLPIYDMVKENKFRQDLLYRINTIEINMPPLRERD
                     DDVVLLANHFLNYYSKKYNKSVNKISDAAISRMKKYSWPGNIRELQHSLERAIIMSVT
                     SVLQPEDFIFNSKGQEKDEDAFLIENFHLEEVEKVLIRKVLKKYNGNITQAATELGLT
                     RSSLYRRLEKYGL"
     gene            26810..28057
                     /locus_tag="VGA21_11995"
     CDS             26810..28057
                     /locus_tag="VGA21_11995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008199804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEX9651275.1"
                     /translation="MDKKIKKKIWTLKRLSLIAFGVLFFSFILYNFIWADRRSKLNVN
                     REKITIATVDRGPFQEYIPQTGTVFPKETFYLDAIEGGTIKRVVKESGANVNPGDVIV
                     ELSNLNRELTVMSQEASYNESINRVRQTRLGVTQNDLQQKAALAQIENQLAILKPQYE
                     REKILFEKGLIAQQQFERTKADYEYNVKRLEFTYTSYREDSVARIRQLRELDDSERRM
                     KQSLEGVGKILDNLFIRSPIEGQLTTPLLVVGQSINQGQRIGSVDIEGSYKVQCLIDE
                     LYLPKISTGLTATFDFNNQSYSLEIGYIYPGVDAGRFSVDMFFAGEQPAGIRRGQSLR
                     LKIALGGSSEELLLPVGGFYKDTGGNWVYVLEDGGKAVKRDVRLGRRNTEFFEVLEGL
                     DPGDRVITSSYDNFGNNEVLILN"
     gene            28079..28780
                     /locus_tag="VGA21_12000"
     CDS             28079..28780
                     /locus_tag="VGA21_12000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008627067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9651276.1"
                     /translation="MSNTMIKIKGLTKLYTTDEVETTALNSIDIEIQEGEFVAVMGPS
                     GCGKSTLLNILGLLDNPTDGEYWFGEHEVSKFSERQRAQLRKGSIGFVFQSFNLIDEL
                     TVFENVELPLLYLKVPSKVRKEKVEEVLERMNIMHRRNHFPQQLSGGQQQRVAIARAI
                     VARPKLILADEPTGNLDSTNGEEVMKLLAQLNEEGTTIIMVTHSPYDANYAHRIINLF
                     DGKVVTENIKEQFHI"
     gene            28904..31282
                     /locus_tag="VGA21_12005"
     CDS             28904..31282
                     /locus_tag="VGA21_12005"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9651277.1"
                     /translation="MLKNYIKITIRSLMKSKIFVFINIIGMGLALACCVVAYLNYKYA
                     YGFDSDQLNRKEIYRIDFTRVFEGRSRDWGITPLPLGTAIKENIPELEEVVRYHPTGG
                     NIRIEEDLFNTNITYVDEKFFEVFSFPLKYGNSNELSDPSKIFISENLAAKYFGNEDP
                     VGRQITQVLDSGKREYVVAGVIARMQSNSSFTFVESITNWANYKGTYPNLDENNWGAW
                     STVFLKISDPSRINAIEGQLQQYVEPQNLAREDFKVTSYYIEPFDGMAERTREKEMDS
                     YTREGLPTPAAIVPAIMAVLILLIACFNFTNTSIAISGRRLKEIGLRKVMGGRRKQLV
                     IQFLAENIALCLIAMIAGLLMAEYLVPKYSQMWEFLDIELSYVNNVGFFGFLFVLLLF
                     AGLVAGSYPAFYISRFDPTRILKGTAKFGGAGAIPKLLLGLQYVISMLAIIMGIAFVQ
                     NARYQEELDLGFDKAGVIYTYVNNFNEFEIYRNALAENPDIEVIAGSEYQLYSSGRND
                     PVRYEDKQIETDILHVGDDYLKAMSIDIIEGRSFAEDSETDRLESILVSPSFISQIGW
                     TDNPIGKRIVWADTISLYVIGVVKDIYTNGLWDELDPMIIRYVPEKDYRFLTVKTSAM
                     KILEVNEFMKDKWNEIFPTRLYSGRSLDEVLRESAEVNENIIKMFLFLGIIATILSAT
                     GLFTLVSLTIIRRMKEIGVRKVLGASVVNIAKNLNRQFFIILVIAAILGSVLGSYMGS
                     MLMDSIWKYYVAMNIPSAIISVLLMLLISLITVGFKVYNAAMLNPVNTLRSE"
     gene            31346..33733
                     /locus_tag="VGA21_12010"
     CDS             31346..33733
                     /locus_tag="VGA21_12010"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9651278.1"
                     /translation="MIKNYLSIAFRNIRRNSLYSIINILGLSVGIACCLLILSYVRYE
                     LSFDLPHKKNDRIYRVYMDANFGGQQALIGVTPNIIAPILQSQFPEVEKVVRVYNIGK
                     FRPQIVKVKEELYEEKNFYYADSSIFEIFTFQPVYGQLDGSLDKPNTLVLTQSASQKY
                     FGNKNPVGSVVNVNGNTDYEITAVIEDMPNNVHFRFDMVGSFQGLSWAKPENLTFGSA
                     NFYTYVLLYPGADHQNFEDNVATYTENTYGGQWKQSGFFLTLKMEPLKDIYLRSPIDN
                     DIDKKSDIRYVYIFSFIAILVLVIACINYMNLATARGSERAKEVGMRKMLGARKNELI
                     SQFMGESILITGFAIFSAIVMVELLMPVFRELSGRQLQNSVSEPILISSLAGIWIGVS
                     LLAGFYPAVILSSFKPSKVLKGSFKSSAAGRGLRKILVVLQFAISIFLIAGTIVIFRQ
                     LDFIQNKKLGYDKDQVIAVPIDNKIAGNLDIFKNEFVSIPGVINVTAASETPSDVKGG
                     YSYIFEGRSLDESMSVNAMAVDIDFIKTLGIEVVSGRDYSEDSKQKPDFSFIFNEAAA
                     SRSGLSIENLIGERISLNGRNGVVTGIMKDFHFTSMYREIGPLILFIEPSQFAYMMIK
                     IDGQNIQQTLNTLATKWKTLASHRPFEYGFLDDEFTRLYAAEMKVGELFSVFAIVAIF
                     IASLGLLGLAAYMASQRAKEISVRKVMGASVGRVVLLLSFDFARLVLIAFIISAPLAY
                     YIMNNWLNEFVYRVSFGVETLLIAGLLAFIIAWGTVAWQAFKAATINPVDILRYE"
     gene            34037..34267
                     /locus_tag="VGA21_12015"
     CDS             34037..34267
                     /locus_tag="VGA21_12015"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX9651279.1"
                     /translation="MKPQLTTRELQILKLILKQKTSREIGDKIGISRRTVEKVRASLL
                     EKTKSVNAVGLVAWAINNDIVSVSSKKPRIRL"
     gene            complement(34442..37513)
                     /locus_tag="VGA21_12020"
     CDS             complement(34442..37513)
                     /locus_tag="VGA21_12020"
                     /inference="COORDINATES: protein motif:HMM:NF027232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651280.1"
                     /translation="MKFAKTSILTVLALIAVPFEGQSQSNVNSDNEKLYQAYEYRNLG
                     PTRGGRATAVAGHSEKPGFFLMGATGGGVWKTEDYGDSWQNISDGYFSTPSIGAIRVA
                     NSNPDIIYVGTGSDGIRSNVITGKGVFKSTDAGKSWSFAGLPMTGQIGAVEIHPSNPD
                     IVFVAAIGQAFQRNKERGVYKTTDGGKTWSQVLYHSDSIGAVDLEFAPDNPDIIYATL
                     WRAERKPWTIISGSKEGGIYKSIDGGKTWKKQTQGLPTGLIGKIDLAVTPADPEILYA
                     LVEAAPDDVRGLYKSSNRGESFELVSSYRPLTDRPFYYCNVDVNPQNPNTVYVNSTGF
                     HYSVDSGKTWRRMRTPHGDNHDMWINPKDTLVWVQSNDGGANVTINYGKTWSSIENQP
                     TAELYQVEVDDQVPYWLYAGQQDNSTMAVPSLPPYNHPAGDAGHWLAVGGCETGPAVP
                     KPGNHNIVYSNCKGRFGVYDKRTGQERQYYVGAANMYGHNPADLKYRFQRVSPIHVSP
                     HNPDVVYHTSQYVHKTTDDGITWETISPDLTAFDPKTQVISGSPITRDITGEEFYSTI
                     YAIRESSVKAGVIWVGANDGPVHVTRNGGETWEQVTPKGLAAGGRVDAVEPSPHKEGK
                     AYIAVLRYQLGDWKPYAYRTTDFGKTWRLLTTGKNGIPENYPVRVVREDQVKEGLLFA
                     GTEFGMFISYDDGETWEKFQRNLPVTPVTDIKIYRDDLILSTMGRGFWIMDNISSLRA
                     AKSVDAVTLFKSKDTYRFRFRGSNSVPEYPSPGVVIDYYLAHNAKDEITLEIVDARQQ
                     VIRSFESETRRQSQDDEPSMATGFFFRGNPSSLEKTAGSHRFIWDMDHKGAWDSNRRR
                     SFLNGPMAAPGEYQVRLIVDGNTYTQTVKLVIDPSLAASGITYDDLKSQEELTLKIRD
                     LQDTAKRVGNSVQNVRKEIAKELKENSSLKSEDDKWAKIENQMFTADGTYMTPMLLDQ
                     ISYLSSMLNRADQRPGKDAFDRYTELKIKFENILKEYLSLKETSAVAGDGG"
     gene            complement(37611..38213)
                     /locus_tag="VGA21_12025"
     CDS             complement(37611..38213)
                     /locus_tag="VGA21_12025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015814087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HEX9651281.1"
                     /translation="MGKKDPKIDAYIARSADFAKPILKHIRNLIHKGCPEVAEKIKWS
                     FPNFEYKGIFCNMAAFKAHCTFGFWQGALIKDNHGVLTSGEAMGHFGKITSLKDLPSD
                     KIILDYIHQAVRLKDEGIKNPKKTVKRLKEVRVPSEILKIIRANKKAFSVFENFSNSN
                     KKDYIEWIEEAKTETTREKRISTMMEWLQEGKPRNWKYMP"
     gene            complement(38276..38884)
                     /locus_tag="VGA21_12030"
     CDS             complement(38276..38884)
                     /locus_tag="VGA21_12030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651282.1"
                     /translation="MKKEEIAGFIFAFIAYTLKIYRIHFHTYFIVAAIIYLLGLYIYM
                     VTTRKIEMDSFFIGLSSTFLLIWLLFLTKFFPMGIIPMSFSFISLVVGVYFKFRNKST
                     LADGRQWINLVILILCIVIYKMPNDQQYYFTGIKYNYEIESDYMTWAKYSWFLYREGK
                     YVQALSASERALSIANQMNDDFWIEKIIDQQEKIQSKNWTKY"
     gene            39108..41549
                     /locus_tag="VGA21_12035"
     CDS             39108..41549
                     /locus_tag="VGA21_12035"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9651283.1"
                     /translation="MIHHYLKIAFRNLVRQRFYTLINIFGLALGLACTVLIGHYIHYE
                     LSYDTIHPDVERLYRVNQTNIWYPEQGAMSSTGLPLAKILADDFPEVESSLRINTPYR
                     FTVRYDDSFGNIKAFNEDDFLASDSNFFEFFEFKLKEGDPATALKGLNKVVISDEASA
                     RLFGEEPGLGKIILMGEDRIPLEVTGVTESQPENIHFDFDYLISIYTNPNIKRFEWSW
                     IWTQAVTYIKLKEDANPEDLEGKMESLVDLHVAPSFKRFGIDFSEFIKGGSWNFYLQP
                     VTRIHLYSAVEGNRIGNTGDIKYIYIFGLVGIFILMIAVINFINLATARAGIRAKEIG
                     IKKVMGSQKRNLIMQFLSESMLVTFISLGVGLAVMEIIRILLNKYLLIPMLFSIWENP
                     VLLLIMPLFAIIIGILAGLYPAFYITRLAPGEILKGRITRGVAKSNLRNLLVVVQFTI
                     SIILIAATLIVSQQVNFFLNSDLGFEKENILLINNSERLGGQLQSYVDQLDSETAIKG
                     AAISMLRPGRGGFEDIYSVEGYEEKYPMSTEKVDANYFSIMNLKFRAGRPFAENSISD
                     RNSVIINESTARLFNWTPEEALSHRLNDLGDELEAVPIIGVVKDFHFQPLQNKIGPLL
                     IFHENGAMWGDMRVVIVKYDTKMESQLVNLLQSKWHQILPDTPFEYEFLDQTLAEQYD
                     FEKQLGGLFSFFTTLSIVIAIIGLIGLVAFASEQRRREIGIRKVLGAPVLRILVMLNS
                     HHLKLIVLSLIFAVPVAWYAMDNWLSGFPYKIEISPWVFLLAGGSVAFLSLLCVSYLS
                     IRAASLNPAEVLKEE"
     gene            complement(41666..43345)
                     /gene="recN"
                     /locus_tag="VGA21_12040"
     CDS             complement(41666..43345)
                     /gene="recN"
                     /locus_tag="VGA21_12040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009581430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEX9651284.1"
                     /translation="MLKELRISNFALIKELRFSPSEALTIITGETGAGKSIMLGAVGL
                     LLGERADTKTLYDAGDKCYVEGDFNISEYDLAGFFKDYDLDYDKICIIRREISPSGKS
                     RAFINDTPVTLDILKELGNNLMDIHSQHDTLLLRSSDFQLEVLDSYAGTSEILANFKS
                     AFAKYRHSEKNLKQLREEHSRLSAEFDYINFQKEELDKTNLVEDEQENLEEQVQVLEN
                     SEEIKSNLNLILNSMQDSEISVLNQVKESLRLLKHLTKFSSRYELFSSRLENLSFELE
                     ELIRDLATEEENVETDPARAEELKSRLSLIYGLQQKHKVSTIKELVEILEVLEQKVNT
                     ASGMESSIKEAEAKVRKFWDEIEKYGRLLSEKRRKVVPELQKELTGYLKDLGMPDAAI
                     VIEITDVEAGNQGMDKVSYLFSANKGIKPKELRQVASGGEFSRLMFCLKFILAQKTRL
                     PTIVFDEIDNGISGETALKLGNMMKAMARGHQVITISHLPQIAARGSNHFFVYKENLD
                     GKAVSKIRELSEEERVAEIAKMIAGDNPTQNAFKSAKELIANKLTGRETGS"
     gene            complement(43412..44317)
                     /locus_tag="VGA21_12045"
     CDS             complement(43412..44317)
                     /locus_tag="VGA21_12045"
                     /inference="COORDINATES: protein motif:HMM:NF027445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4835 family protein"
                     /protein_id="HEX9651285.1"
                     /translation="MRYWNISASLFATTILFHSSSAQELNCSVRVNADQIALSDKRIF
                     TDMETSFEQFLNSRKWTTDTYRQVERIKCNINVTIDKMVSIGTYEATVQIQSARPIYN
                     TNYESILLNFADREWQFDYTESQPMDFVETRFENNLTSLLAYYAFIVLGFDYDSFSEL
                     GGTPFFNKAMNVVTEAQPSNFLGWSPQRSNRNRYWLAQNLTGQALEEIRQGIYTYHRL
                     ALDTYLENTPDAQQKILEVLTHLKQLNDRNPSQILITAFVDAKTDELVNIYGDASPNV
                     KRQAHSLLISIDPAKKNRFDKILQN"
     gene            complement(44292..45491)
                     /gene="coaBC"
                     /locus_tag="VGA21_12050"
     CDS             complement(44292..45491)
                     /gene="coaBC"
                     /locus_tag="VGA21_12050"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009581433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HEX9651286.1"
                     /translation="MLKGKKILLGVTGSIAAYKSALLTRLLVKEGADVQVIMTSSAHD
                     FITPLTLATLSKHPALTDFIRNDAGEWNNHVELGLWADLMIIAPASANTLAKMANGQC
                     DNLLLAIYLSAKCPVMIAPAMDLDMYAHPSTKNNLMKIGSYGNILIEAEDGELASGLK
                     GLGRMSEPEHILKEIQGFFLIKKRFKGKKVLINAGPTYEPIDPVRFIGNHSSGKMGYA
                     IAERFAEMGAKVELVSGPTYLDVKSDNIHITRVTSAKEMLKACQKHFKSCDIAVLAAA
                     VADYSPSNFTRHKIKKKTGVLSMELASTPDIAKELGKVKKKNQVLVGFALETENETRN
                     AAKKLKEKNFDLIVLNSLQDKGAGFGHDTNKISIFRKDNKKKYFELKSKKDVANDIIN
                     EILEYIG"
     gene            complement(45495..45815)
                     /locus_tag="VGA21_12055"
     CDS             complement(45495..45815)
                     /locus_tag="VGA21_12055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010856660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEX9651287.1"
                     /translation="MANPSIITRDLTGLSKEVGNIYETVAIISKRSRQIATNLKEELN
                     SKLAEFASTVDNLEEIFENREQIEISKFYERLPKPTIIATEEFTDGKVLYRQAEEEEG
                     GIKL"
     gene            complement(45827..46633)
                     /gene="bamD"
                     /locus_tag="VGA21_12060"
     CDS             complement(45827..46633)
                     /gene="bamD"
                     /locus_tag="VGA21_12060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009581435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HEX9651288.1"
                     /translation="MRNQQGWIVLVLLVILTSSCGKFRKIQKNPDWRVKYEAAMKYYE
                     KEDYFKANLLLEEVLPIARGLPESETILFYYAYSYYHQGQYLLASHNFRTFYQTYSRS
                     PLAQEAEFMYAYSLYRESPNYNLDQSSTVEAIVAMQIFLNKNPASRYRPEAIEIIDQL
                     QVKLEKKAYENSKQYYKVGRFQSAVIALDNFTKDYPASVFAEEIYFLKLDAQYNLAKL
                     SIPDKREERYRETVRLYQEFIEKFPNTSFRKDAESIHEDSSEKLNKIAEN"
     gene            complement(46714..47424)
                     /locus_tag="VGA21_12065"
     CDS             complement(46714..47424)
                     /locus_tag="VGA21_12065"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEX9651289.1"
                     /translation="MKKSGIAILVILAFSPAISQSLKLLDNEQNWEGKIGDKIQATIR
                     IQNISATPVSIVIQRVKTEIGTTQLTRLCWSGDCLEPDAQKLIISKKLEPGEITSNFV
                     SILDAGLTEEFSIVSYHIYNKDNPDDAISFDVHYSVKEAARGDVFYESAEIFLRDIYP
                     NPASENAFLDYQLKVEEQEVKIIIHNVLGSIVGVHPLSFHESQLKIPIQDLNPGVYFY
                     TLYLNTRSVTTKKFVVKK"
     gene            complement(47586..49067)
                     /locus_tag="VGA21_12070"
     CDS             complement(47586..49067)
                     /locus_tag="VGA21_12070"
                     /inference="COORDINATES: protein motif:HMM:NF024500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OstA-like protein"
                     /protein_id="HEX9651290.1"
                     /translation="MNSIAQDRIRIENSREFIGGKLNGEDYQKLIGEVIFKREDTWIY
                     CDEAIYFKKRSYIEAKGNVHIIDGDSVDITSEILIYDGNTRVAKLRNNVIFTKLGIMT
                     LYTDNLDYDRVSQVANYFSGGKLVDSTNTLTSRRGYYDVNTNMASFKHNVVGKNKNYT
                     LTSDTLQYNSATKIIYFRDRSILIDDEGNVLEYDEGIYNTLDETSLLERNIVRTESYI
                     IKANIFNLDDKKQEYRAKRNVELVDTENDIIVTGQSAIYSKRTGITKVFDQPVLKVLM
                     DGDTLFLSADTLVAYDSDIEAKKKLIAYHNVKFFKEDLQGRADSLIYEVSDSLIHFFT
                     NPILWTEGNQIIADTISAFIVNKKIDKMKLVSNSFIVSQDTLSNFNQVKGRNVLAFFK
                     DNKISLVQVTGNGESLFYALNDEETDLIGLNKSTSSNITINFENNKAKEVHFYTSVEA
                     SFIPPHELKPADRNLKGFTWRIKEKPTLMEVLGELKLLQKLVN"
     gene            49057..50535
                     /gene="tilS"
                     /locus_tag="VGA21_12075"
     CDS             49057..50535
                     /gene="tilS"
                     /locus_tag="VGA21_12075"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEX9651291.1"
                     /translation="MEFIAMPQRLTNNIDIFACIVIAKRQGLFKQANVAKVANKMPHN
                     SESSNTHEFERAFQRFIIKEKLVPTRSKILLAVSGGVDSMVLLHLFHSLKYRIGVAHC
                     NFGLRGKESDYDQKFLKEYTRKNNIEFYNKRFQTKEYAKKGKLSVQMAAREIRYFWFE
                     EIRRKHSYDLIATAHHKDDAIETILLNMFRGTGIMGLAGILPQRGCIIRPLLFANKNA
                     VIEYARSMNLAWREDSSNLDNKYSRNLLRNRIIPNLIKENPGLRKVLDNSLSRFRIAA
                     QLLRDYSALIKMKFVEMQGEVFSINMKIRNIAYSEPVLYAIIKDYGFNYTQVKEMLHA
                     GSSPGKKFCSDRYCIFADREKWILIEKEIEVQEKLLIRKSTKRKKSRLGDFNISRERG
                     LKKIIGDFNTAHLDFDKLRFPLEIRAWKEGDKFQPLGMRGKKKISDLMIDQKIPVNLK
                     RRVKVITSGDDIVWVVGIRIDERYKITPQTREFYIMKFVSND"
     gene            50528..51046
                     /locus_tag="VGA21_12080"
     CDS             50528..51046
                     /locus_tag="VGA21_12080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020530690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX9651292.1"
                     /translation="MINPFKKTFTPQEMSLFRFLSKVKVFEDLDYKELSYFVPFLHER
                     EYKRDEIVFFRGDPSQAFYIIKKGRVSLSVDIEDKFEMLDELKTNQSFGDNAFLNNTR
                     RIYTSMITSDEAILYVIPRVNIHEIFDREVEVKAKVMQSLSSQYNEYTTNLFKAYQSS
                     FGFFDLGKVYKS"
     gene            51129..52064
                     /gene="mdh"
                     /locus_tag="VGA21_12085"
     CDS             51129..52064
                     /gene="mdh"
                     /locus_tag="VGA21_12085"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014020429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HEX9651293.1"
                     /translation="MKITVVGAGNVGATCADVLAYREIANEIVLLDIKEGLAEGKALD
                     IWQKAPINQYDSYTTGVTSDYSKSAGSDVIVITSGLPRKPGMSRDDLIETNAGIVKIV
                     TESVVANSPNSIIIVVSNPLDVMTYQAHITSKFPRTRLMGMAGILDTARYRAFLAEAL
                     QISPKDIQAVLMGGHGDTMVPLPRYTTVAGIPVTELIDSEKLNAIIERTKTGGGELVK
                     LMGTSAWYAPGSAAAQMAEAIVKDQKRVFPVCIKLEGEYGIDNCYLGVPVILGKNGIE
                     KIIELQLNDEEQAMLNTSRDHVTEVMQVLDRLNSK"
     gene            52084..52644
                     /locus_tag="VGA21_12090"
     CDS             52084..52644
                     /locus_tag="VGA21_12090"
                     /inference="COORDINATES: protein motif:HMM:NF022588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HEX9651294.1"
                     /translation="MRKLYKVFLLLIFLSVFELANGQDVKLPGLDPSPADIAILRESR
                     NADPHAKIIYSRPQKKGRVTIAADNGALAPFGKLWRTGANETTELIVYKDIKLGGVDI
                     AAGTYSLYTIPGEKEWTLILNGKLNTWGHFAYDESQDKYKITGAVKSYEPAVEAFTIV
                     FGGVSGGKSTLYLAWDTTIVEIPVEL"
     gene            complement(52806..53198)
                     /locus_tag="VGA21_12095"
     CDS             complement(52806..53198)
                     /locus_tag="VGA21_12095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009577792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HEX9651295.1"
                     /translation="MIKEQLKIMIQLAVSDGMIAEKESRLIMTVGLANGLSKEVIKGM
                     MEAPEPMGSLSGLTDDQKFEYLYNIVQMMKVDGQVFKSEISFAEDVAAKLGYKKEAIS
                     ELSSRIYSDPSITSHREDLKKRVNKFLV"
     gene            complement(53317..53463)
                     /locus_tag="VGA21_12100"
     CDS             complement(53317..53463)
                     /locus_tag="VGA21_12100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651296.1"
                     /translation="MAIRKNLALIFGIIIAVVILFQAVARENQVNRTDSANLDSKIEH
                     FSSL"
     gene            53589..54278
                     /locus_tag="VGA21_12105"
     CDS             53589..54278
                     /locus_tag="VGA21_12105"
                     /inference="COORDINATES: protein motif:HMM:NF022441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HEX9651297.1"
                     /translation="MKLIISRTLIGIFLLASFSVLSQDREERSLDSFSKMRVGEGIKV
                     ELKKGTSEKAIIEVRGLDVDDVVTEIRGGSLNIYLDGNWHRNIDVSIELTYKSLNSIT
                     ISSAGNVRTNEAIESDDLYISASSAGGGDIEVKVKELELEASSAGTLDVYGSTIELEA
                     AASSAGSIDAYDLKAEEVVVRASSAGSIKVQATKLIDARASSGGSIRYRGNPSKEYTN
                     SSSGGSVRKSG"
     gene            complement(54295..55944)
                     /locus_tag="VGA21_12110"
     CDS             complement(54295..55944)
                     /locus_tag="VGA21_12110"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEX9651298.1"
                     /translation="MNRILMIIGTLLLVNSCQTKTEPEFTGHVAFLASDDLEGREAGT
                     TGEQKAAAYIVEQFQKIGLKPLGDDNTFLQAFDFLASKEVGERTTLSIDDNQVNNENF
                     YPLNFSGNTMAEGKIIDGGFGIHALELGYDDYAKLTELKNKILLINISSPDGIHPHSK
                     YLDYHNLKERARVAEEKGASGVIFYNIDATAPDPEKHYTQKITSLEIPVTFIKNETSE
                     TLAGKNVIITVELVEERRVGHNVIGWIDNGANSFAVLGAHYDHLGYGEGGGSLYRGDD
                     KMIHNGADDNASGTAMVIELAKGVGRSGLINQNYVFILFSGEERGLLGSKYFVDNPTI
                     DLSKIKYMLNMDMVGRVDTTKNAISIMGSGTSPQWDSLLNLQTTPFDLVKSASGVGPS
                     DYTSFYLKDIPALGFFTGTHSDYHKPGDDSETLNYEGMMQVYQLIFNIVSDLNDFETI
                     AFSKTVDSNARSAPSFTVTLGIIPDYVYEGKGLKIDGVTEGKPGAVAGLQKGDIITRM
                     GELSIDDIYAYMNALSKFKQGEEIEIEILREGIQTVLKTTL"
     gene            complement(55953..56312)
                     /locus_tag="VGA21_12115"
     CDS             complement(55953..56312)
                     /locus_tag="VGA21_12115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651299.1"
                     /translation="MKYLLAVLFLFPISFAYAQEEEIWYVWIDTEIKQKDNTIRIVSE
                     EPIRITCCVKSPKYRRFQKQTVKWIKKNYDLAFNLEAPLKNIEDKSFAETVIKEAIEK
                     SGDDESIIIIKYSATCK"
     gene            56775..56963
                     /locus_tag="VGA21_12120"
     CDS             56775..56963
                     /locus_tag="VGA21_12120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010853463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEX9651300.1"
                     /translation="MASQKLVRSKDKIIAGVCGGIAEYMGVDPTVIRILYVFASIFTA
                     AFPGILVYIIMWLIMPEN"
     gene            complement(56944..58524)
                     /locus_tag="VGA21_12125"
     CDS             complement(56944..58524)
                     /locus_tag="VGA21_12125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX9651301.1"
                     /translation="MNKQAILFSTEFVFSDQNLRSIFDSFPFALAITTLEGKILMFNN
                     VAVQLTGYSRDELLNGGDIRNLYYDPNDRLRIMAELKKGKIIKDEKILIKLKDGQPYP
                     VFLSISLLQSENEMLLLGTINERSVDYKVSPEILGVVLDDSGNKEENDPQKRLVKSEE
                     RLEFALTGADLGLWDWNIETDRVIINTKWASMLGYEVSEVAANLSKREMMIHPEDKKS
                     RITALNNHLSGKTPFYASEYRIKTKDGTWRWVLDRGKITKKKSDGTPIRMTGTHLDIH
                     YRKEAERKLVELTKSLQQSNYGLKQFAYITSHNLKSPVNNISSLLSLVDQSRISADNK
                     EMLSKINQLASTLNDTLDDLTKIISVSDGSSQETAEVLNFKSVIDSVLVSLGPMVQES
                     NASITLDLKIQEIRYSKSFLYSILLNLIGNAIKYRSAEKQPEIILATRKENDNILLTV
                     QDNGLGIDLSIHKDKVFNLYNRFHNHVEGKGMGLFLIKSQVESLGGSVDLKSKPGEGS
                     TFIIRLAEQRPTVSSQAL"
     gene            complement(58699..59895)
                     /locus_tag="VGA21_12130"
     CDS             complement(58699..59895)
                     /locus_tag="VGA21_12130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013407957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEX9651302.1"
                     /translation="MNDYYQVLGIPRSATAEQIKITFKRLAVRYHPDKNPGDKRAEEI
                     FKEISLAYDVLSDPEKKARHDLVLAYSRTSKSFQSQTTNYPRYNTYQRRPYSPPGKYQ
                     YQFGWSYVRNQLLAFGFVFVVAIIVLSVQAAYEYRLRKEKERIDAWRNQRMQMAIMTF
                     ENGKIRPAFDTLRYMIEEFPGEFELKDQKNKLLTKVKVAADQQFRRKDFSRAAFLFTV
                     ARDYEEPYLLNLDVHFKLAQSYENLEEYEKAAETINYVLARDSENINLNLELANLYTN
                     RLDEPEKALEFFDRARDKIRGLLEAAYGRAYELVINPVKMPDLYYHVYRGRGIANTKL
                     GNHSDAIKDFNWAILFRPGHGEDYFYRAENYFALNDLRRSCRELDKAIEAGFEAAIVT
                     RRQRCR"
     gene            60025..61032
                     /gene="msrP"
                     /locus_tag="VGA21_12135"
     CDS             60025..61032
                     /gene="msrP"
                     /locus_tag="VGA21_12135"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF003767.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HEX9651303.1"
                     /translation="MANFIIPRSWQRIRERITDESIYQNRREILKKLGLAGGGLLMSP
                     FLFPSCTSSNGSASESVSQADNNFSFPGMMDLYPPKKNETFPLDRPITDEYLATHQNN
                     FYEFIHPNDPNIYNAYKYTQDFDTRDWSFEVSGLAGNTGKFALEEMIKKFGLEERTYR
                     FRCVEAWSMAVPWTGFSFASLVKFLKPESSARYIRFLSAADENQMPGVKNQPWYSWPY
                     FEGLRMDEAMNELAFMATGIYGKPLPKQNGAPMRLLVPWKYGYKNAKSIVKIEFIRGE
                     PETFWHKVAPSEYGFYSNVNPGVPHPRWSQAQEQMLGEDTKRPTLLYNGYGEWVAQMY
                     S"
     gene            complement(61029..62243)
                     /locus_tag="VGA21_12140"
     CDS             complement(61029..62243)
                     /locus_tag="VGA21_12140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HEX9651304.1"
                     /translation="MKKLALGIILISICSCNSKHEENSAVESAPMILEEIRFPGNRAS
                     EPNLTVGPDGTIYLSWIEDQNDNSTLKYARLNNGNWVSETQIASGANWFVNWADFPSM
                     IALESGYAAHFLAYISEGKYSYGVNLASSKTGKDWNNPVMAHDDNTPTEHGFVTLLPM
                     SRENYLAVWLDGRNYVDSEHKPATKEMSLRCGLFDLDGNKLEEHVIDSRVCDCCQTDG
                     AISSRGPVIVYRDRGLMEVRDIFISRFENGEWKEPWIVHPDEWEIPGCPVNGPAVDAS
                     GSNISVAWFTAARGTRKVLVSFSGNDGDQFGEPVRIDLGNPLGRVDLIQFDNFTAVTW
                     MEENGETAFIYCRKIFRDGTLGEVIPINETDKSRSSGFPRMTRDKESLIFAWTVPGEV
                     STIQTVKGNFTD"
     gene            complement(62270..62686)
                     /locus_tag="VGA21_12145"
     CDS             complement(62270..62686)
                     /locus_tag="VGA21_12145"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019945408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HEX9651305.1"
                     /translation="MKKILILCTGNSCRSQMAEGYFRKFAGNNAKIVSAGIEAHGLNK
                     NAVKVMNEDEVDISTHTSKTIDDLEESKFDFVITVCDNARESCPVFPSEVKTIHQNFE
                     DPAAFKGDEKNVLQKFREVRDQIKSFALKFVHLNLH"
BASE COUNT        18854 a        12257 c        12498 g        19145 t
ORIGIN      
        1 gttagcttct gcaaaagcgg gtcgtaatcc gcaggtgccc gaggtctgta tttatttcaa
       61 ttacttactc tttcgcggta acagggtgaa gaaagtggaa agcaatcatt ttgacgcatt
      121 tcgatcagaa aattatccgg cactggctca atcaggtgta agaattgagt acaatgttaa
      181 cttcattagg aaagttcagc aggggaggtt aaaggcaaat gtgaaaatag atactaatgt
      241 tagcctaatc aaattgtatc cgggaataaa ccggttaatt gttgaagcta tgttgcgtac
      301 aaagaaactc aaaggtgttg ttctggaaac atttggatct ggaaatgtcc ccacggaaaa
      361 atggttaatc tccagtttgc agaaagccat taagcgggga gtaattgttt tgaatgtctc
      421 ccagtgccat ggaggcaaag tggaacaagg caagtatgcg gcatcaaaag ttctcaagga
      481 gattggggta ttaagtggca gcgacattac gaccgaagca gccctgacga agatgatggt
      541 tatattcggc aaaggtggct ccctgaataa tcaaaaaaaa cagctactaa agccaatatg
      601 cggcgaaatg acttaacttg cacggccatt aaaaacaatt taatatttgc aaaaagttta
      661 ttttattgat ttatttgtgg cttgtagttt tttaggagag gtgaccgagt ggttgaaggt
      721 gctcgcctgg aaagtgagtg tactcttaca aaggggtacc aagggttcga atcccttcct
      781 ctccgcgggt tggaaaaaga aacatgaatt ggcataataa ttttacttaa tctggataca
      841 taattactaa actatgaaaa aattaattac taaactggct ttcatcggca tactaatgat
      901 cggtttcaca aacgccggtt tcagccagga tgatacaact tctgtcgcgg atcaggatac
      961 ggctgaggtg caagaccagc ctgaagagca accggcagta gaaacacaac ctgtttatga
     1021 ggaacctgcc gatgaatccg ggtcaacgtt tcaccaaaaa gttaaagaaa aattcataga
     1081 aggtggttgg caattcatgg gtatagttct gctttgttta attatcggac tggctatatc
     1141 gattgaaaga attatcactc tcaatctggc aaccactaac actaaaaaac ttttgcagaa
     1201 tgtggaagaa gcgctgggaa gaggaggtgt tgatgctgcc aaggaagtaa ctaaaaatac
     1261 cagggggcct gtagcttcaa tattcactca gggaatgctg aggatgtcag aaggaataga
     1321 aatggttgag aaatcaatca ttgcttacgg atcagttgag atgggaagat tggaaaaagg
     1381 tttagtgtgg gtatccctgt ttatttcgct tgctcccatg cttggattca tgggtacagt
     1441 aataggtatg gtagaagctt ttgatgcgat tgaagcagcc ggtgatattt ccccggcaat
     1501 tgttgcaaat ggtattaaga ttgcattgct aacaaccgtc gcaggtttga tcgttggagt
     1561 tattcttcaa ttattctaca actatctggt ttcgaaaata gacgggctgg tcaatgaaat
     1621 ggaagacgcc tctatttctc tggtcgacct tctggtgaaa cataatctta gcaagtaaca
     1681 tttgttaaat aaattgaaat ggaacaaatt gttaatattg gacttattgc tgcttacatt
     1741 cttgtaatag gcggagggtt aagtgctatc atccttccct tgattaatgc gttaaacaac
     1801 cccaagagtt taatgtatgc cggtatagga attgcggggt tgctgttttt attcctaatt
     1861 gggtatttga ttgctggaaa cgaagtaaca gccaactaca ttaaatatgg agtggatgcc
     1921 ggaacctcaa agatcgtggg aggggcactg atatgcatgt atatccttac tctatttgca
     1981 atcgtaggaa tattgtatac ggaattctct aaactgataa agtaatggcc cgttcaaaaa
     2041 gatcacaacc ggaaatcaat tcaagctcaa tggcagatat tgccttcctg ctgttgatct
     2101 tctttcttgt tacgacaact attgcaaatg ataaggggct gactatccag cttccaccta
     2161 agccggaaga tatggatata attgacataa aaattaagga aagaaattta ttcaaagttt
     2221 atgtaaactc cgctgacagg attttggtgg agggtgaaat tttggaagat atatcggaca
     2281 tcagacaaaa ggttattgac catgttttga ataatggtag ggatcctcaa aaatctgagt
     2341 ccccaaaaga tgctattgta tcgtataaga ccgacagggg aacaagttat gaagtgttta
     2401 ttaagatatt ggacgaacta cagggagcct attatgacat gtacgcggaa cgcgccgggg
     2461 ttacaaacaa acaatggcgt gaaatagcat ctgatcataa agaacctgaa aaggcaaagc
     2521 tttatgatat tggaagagga ctactaccca atggtgacgt gactttccca atgaatattt
     2581 ccatcgctga accatcaaag ataggaggaa attagcatgt caaagtttag aaagaaagcg
     2641 aaaaccaaac aagatatccc aacatcagct ttgcctgata tcatattcat gctccttttt
     2701 ttctttatgg ttaccacagt tctaagggag acaacaataa atgtacagca aagaataccg
     2761 caggcaattg agctgagaaa gctgcaacga aaatcattgg taagttacct gtatattgga
     2821 aagcccataa aaacggaagt tttcggtgat gaaccaaaaa ttcaggctaa cgatgttttt
     2881 attgatacga aagacattgt aatgtgggtt actcaggaaa aagacaagtt ggatgaagtg
     2941 gaaagagatc aactcaccgt ttctcttaaa gtagatatgg aagctaaaat gggtctgata
     3001 tctgacgtac aattggaact gaggaatgcc aacgcccgga aattaatgta ttctgcgcca
     3061 ccaagacgcg cggaaaacta atagaatgct acatattatc agatgaaagt ctttccttat
     3121 ggaaagactt ctttatgaga atgaaaaccg ttgttctgaa taaccagaaa gttattaatg
     3181 cctggtgcat gtttgactgg gcaaactcgg tttattccct ggttattatc tccgcgattt
     3241 tccccgtctt ttataattca gcaactgaag ccgcttttgg cggtgagacc attaatttct
     3301 tcggttatga aatagtgaac agcgtattat attcctacgc actctctttt tcatttctaa
     3361 tcgtggcagc catacttcca ttgctatctg gaattgctga ttacggtgga ttaaagaagg
     3421 gtttcatgaa gttctttacc atgatggggg gaatatcctg tattgcactt tactttttta
     3481 ccggaaagaa tgttgaactg ggaattattg cttccatagt tgccagcgtt gggttttcag
     3541 gcagtttggt attctataat agttacttgc cagagataac tactgaagat aaattcgacc
     3601 gcgtaagtgc caggggattt tcttttggat atataggtgg agttgtgctg ttggttatca
     3661 atctgctaat gattgagttt tatgattcct tcgggctccc tgatagttca tctgctgcca
     3721 gattttcatt cctcaccgta ggcttatggt ggatcgggtt ttcacaaatt acattttatt
     3781 accttccaaa aaatattcat gggaaaaagt cggcaggtca ttttctgatc agtggctaca
     3841 gagaaatagt gactgtctgg aaaagcatgg ctcatctacc taacctgaaa cgattcctgt
     3901 tctccttttt cttctacaat atgggagtgc aaacggttat gtatctggca gttctttttg
     3961 gaaccaaaga attaaatctt gctgattcga aattaatcat atccgtcctg attattcaac
     4021 tggttgcaat aatgggttcg tatttgtttg caatagtctc agagaaaaaa ggcaataaat
     4081 tctctctaac tttcatgata atagtttggg taatgatttg tatagccgct tactttacag
     4141 ccacagaata ccatttctat gctttagctt tctttgttgg aatggttatg ggtggaattc
     4201 aatccctttc gcgcgccacc tattcaaaac ttattccgga aaatactcag gatacagctt
     4261 cttatttcag tttctatgat gttacctaca acatttcaat agtattggga acatttgcct
     4321 acggtgcaat tgaacaattg accggcagca tgagaaacag taccctggca attggtgttt
     4381 tctttctgat tggtatcgca attctcagca aagtgaacat gcctcgctca aaccagaacc
     4441 tcacggtata gcaccagaat aagcaagagg caagaaatga aatatacaat tgtgatgggt
     4501 aatccaattt taataaaatc tgagatctta taaccgcccg gaccaaaaat tatcaggttg
     4561 gtttgatatc cgaagggagt cataaatgaa ccgcttgcgg cgaaagcaat acccaggtag
     4621 aaaggacttc catccatgtt aagagtctga atcaaactat aggtaaccgg gaatgcaata
     4681 gacactgctg ccgcattatt aataaatgaa gtcagcatca ttgtaaatag cataatacct
     4741 gatagcaaaa ccaagggtcc aaatccacct aacaaaccca tcaggccttc tgaaagaaaa
     4801 ataccggttc cggtttttag cattccctca ccaagcacaa gagaaaatac aaggatgcca
     4861 attaaatcga ggtctagttc acgcttaatc tctctcagag taatcaattt aaagccgacc
     4921 atgattgcca gaattaccaa taatgaggag aagagagaga aattaccaac agtcagaagt
     4981 gttattatgg aaactacaat taaagaaagg gcgataaatc tggtacgatt tgaccctact
     5041 gattttggta tactactaat gacataaagg tcacggtaaa gatcaactct ttctgaaaaa
     5101 tcatgtcccg cgtagagcag caataaatca ccaggtctaa gtttcagatc tccaatcctg
     5161 cctgttagtc tttctccggc gcgatgaatt gctacaactg cagcatcata ccggtggcga
     5221 aagtcagatt gtttaatggt tttcccggtc agtgttgaat tctcggcaag cacacactct
     5281 acaatatcct gactctcaac agattgtata tcggtaattt ttggcggcaa tattagtccg
     5341 agatcagaat ttacaagttc aacaatttgt tccgtgctac cggcaaaaaa cagtttgtca
     5401 ttagcttcaa ttttattatc cggtgaaaca ggataaataa cttctttatt gcgaattatc
     5461 tcaaccaggt agacgccttt tagattccgc aagccgcctt ctttaactga tttgcctacc
     5521 aggtgtgagt ccccggcaat atgagtttca ataatgtatt ccctggtgtt cttctcaaat
     5581 tcttcaacca ggtccttccg atcaggaagg agaaagggtg caattatggc tataaatagt
     5641 acaccaacca gggttgagcc tgctccgacg aatagtaaat caaaagggtt caaaccggca
     5701 atggagaatt catccagaaa tccatttaat aataatgtgg tgctggtccc gataattgtg
     5761 agcatacccc ccataattgc agcataagaa agcggaataa gcaatttgct tgaagatatc
     5821 ttattttttt taccccactc aactacataa ggcgacatca aagcaactac cggtgtattg
     5881 ttaataaagc ttgaaagaat ggccacctgg cccatcatcc ttatcaggaa aactttgtat
     5941 tttttagtcc ctccaaagag attatcaaac agaagttcta gacgaaaatt cttcctgatg
     6001 gcagaagtta taagaatcag gagcatgata cttgcgatct tatcattaga gaaaccggca
     6061 agaaggtctt ctggcagaat gatgccgaat atgagtaaga ctacaactgc gagcaaaaat
     6121 cctacagaag gccttacaac ttctttgaat atcaaaacca gagtaacggc gattagaata
     6181 agaaaaaagt aagcctggac ttcgtagggt atcatttcaa ttagtatcta agatgtttta
     6241 cggaatctcc tgaattaatc aattcttgca atgaatcaat tccaatttgt aaatgcttgc
     6301 ctacaaagct ggtggtaact gaatggtcgc ttttacttgt tttaactcca tggggtacca
     6361 tgggattgtc tgagacaaga agcaaagcgc cggttggtat ttcattttta aatccggtga
     6421 taaaaattgt tgcagactcc atatcaatcc ccatcaccct tatcttccta aggtatttct
     6481 tgaaacttgt gtcgtgctcc catattcggc ggttggttgt atatatggtt cctgtccagt
     6541 aatccatctg atgcttctta atcatagagg agacagcacg ctgcagactg aatgatggta
     6601 atgcaggcac ttcaggagga aaataatcac tgctggtacc ctcaccccta attgctgcaa
     6661 tgggaaggat aagatctcca atttgagttt tcttttttaa tccgccacac ttgccaagaa
     6721 ataggacggc tcttggtttg atcgaactta gcaggtccat gactgtagct gccataggac
     6781 ttcccatgcc gaagttcagg attgttatgt catcaaatgt ggcagtttgc attggttttt
     6841 ctttgccaac aatttcaacg ctaaatttac ttgaaaataa ctccacgtaa ttaatgaaat
     6901 ttgttaggag tatatatttt ccaaactggt caagttccat tcctgtatat ctgggtaacc
     6961 aatcggtaac aatttctttt ttcgttttca tcataagatt taggtatttt agaatctatg
     7021 gagatcttaa attttcctga atttcgattt gattttaaaa aacaagaaga taaaattcta
     7081 atttatgaca ttattcgaaa gaaacatgta attcttactc ctgaagaatg ggtaagacag
     7141 cacctggtac atttccttat tttcaaaaaa ttctttccca aatcactcat gaaagtagaa
     7201 tcgggactga attaccacaa aaaacaaaaa agagcggatt tgatcattta caatgattcc
     7261 ggagtcccat tcattatagc tgaatgtaaa tcgcccaaat caattattaa tcaaaatgct
     7321 ttatttcaaa taggcgttta tcaaaaatct atttctccga agtacttaat tgtaacgaat
     7381 ggaattaccc attttatgtg gagaaagaac aatgacttaa gcagatatga tcagatcccg
     7441 gaaattccgg aatgggatta catttcctaa aagaatttat caaccggagc aaaaggtaaa
     7501 ttgaaagttt cccccaccgc tttatatact atttctccat taatgatatt caatcccaat
     7561 ttcagatcat ggcggtctgt gcatgctttt ttccagcctt tatttgccaa atcaatggca
     7621 tatggaagtg ttgcattggt aagggcaagc gtggaagtat agggaacagc accaggcatg
     7681 ttagctacac aataatgaac aacgccatca ataatataag tgggattttc gtgagtggtt
     7741 ggcttacagg tttcgatgct ccctccttga tcaacagcca catcaacaac aacggttccc
     7801 ggtctcatgg tcttaagcat atctttagta ataagactgg gtgccttggc accatgaatc
     7861 agaacagcac ctacaattaa atcagccgtt tgaattaatt ccctgatatt atattcattt
     7921 gacatcattg tgcttacgtt ggcaggcatt atgtcatcca gatacctgag tcttggtaaa
     7981 ttaacgtcca taatagtaac ttccgcccca agacctgccg ccattttagc agcattagtg
     8041 ccaacaactc caccacctag aattagaact tttgccggct taacgcctgg tacacctccc
     8101 agcagcacac ctcttccttt taatggcttc tccaggtatt ttgcaccttc ctgaatggcc
     8161 atgcggcctg caacttcaga cattggtata agcaagggca aacttccgtc agatttctca
     8221 acagtttcat aagcaaggca aactgcctta cttgacatca tagccttagt taagggttcg
     8281 taggaagcaa aatgaaaata agtgaaaaga aggaggtcgt ttttaataag agaatactcc
     8341 tgttcaatgg gttccttcac cttaataatc atatccgctt tcgtgtaggt gctttcaata
     8401 tcgttcaaaa tttcagcgcc ggcgttgcgg taatcttcat ccggaaaacc gcttccttct
     8461 cctgctgaag attgaacata aagtttgtgt ccatgctttg ttaattcttt gacacctccc
     8521 ggggtaactg agaccctgtt ttcgttgttt ttaatttcct ttggaacccc tatgatcatt
     8581 ttcaagattt gatttttaag gccacaaata tagtaagatg tacttgaata ataacttatt
     8641 aatagctagt aaaacacgag tatattatta taattaaaca aataattaat taatattatc
     8701 gaagtaaaat agtactataa ttatctttat ataataaaaa ataatacaaa tatgaatatg
     8761 gtttcaaaga tagatgagtt tgaaattcct gattatgaag atgggtcaaa attgaaaata
     8821 attgttaatt cctgcagcga gcttgggaac tccgcaaagc ctggattgca aatctttttc
     8881 atgggtagtt ttgttaatta tgagccaaag attgctgaga agtggcgata tgaattgaac
     8941 aaagcgagtt cagaagaatt attgcttcaa gataaatcct ggataccgaa cgaagagaat
     9001 tttataaagg tttttttcgt caattcaaca ccagctatgg cgaaaataat gataaaaacc
     9061 agatcttcgg aggtaatctc aaaacagtac gagcttccct tttaagcaag gcaagatggc
     9121 cataagcctg ttgttacgtt tgattcaagg tcgttttgtg ctatttttgc taaccttcat
     9181 tagagcaatc attgtttcaa aacggatatt ttgagttcca cgaaagcagc catagaaaaa
     9241 tcaaaaacga aaaggacatt gcctggtaag gccgaagtgt tggctgatta ccggctggca
     9301 gttgccagca gggaagccag cctgatcggc cgtaaggaag ttttcatggg caaggcaaag
     9361 tttggcatat tcggtgacgg caaagaagtt gctcaacttg cgatggcaaa agtttttcaa
     9421 aatggagata ttcgctcagg gtattaccgg gatcagactt tcatgttcgc gataggagag
     9481 cttaccatca agcaatattt tgcccaactt tatgccgatc cctctgtggt agcagaccct
     9541 tcatcagcgg gaaggtcgat gaatggtcat tttgcaacgc gcatgctgga tgaaaatggt
     9601 aaatggaaaa atcaaactaa ttacaaaaat agcagcgcgg atgtatctcc aaccgcatca
     9661 caaatgcccc ggcttttagg tttagcatat gcatccaaac tttatcgcaa caataaagaa
     9721 cttggccgtt taaaagattt ctcaaaatca ggaaacgaag ttgcgtttgg aactattggt
     9781 aatgcatcca cctcagaagg aatgtttttt gaaaccatga atgctgccgg tgttctgcag
     9841 gttccaatgc tgatttcaat atgggatgac gattacggta tttctgttcc taaagaattt
     9901 catacaattc gtggaagtat ttccgcggct ttggaaggat ttggaaggac agataatatg
     9961 cctggttttg aaattcttaa agtgaagggg tgggattacg aaggtttgat tgaaacatat
    10021 caaacagcag aaaaagtttg ccgtgaagaa catatcccgg ttatgattca tgtaacggaa
    10081 atgacccagc cgcagggtca ctccacgtcc ggatcacatg aaagatataa atcaaaagag
    10141 cgattgaagt gggagaagga ttttgattgc ataagtaaac tgaaagagtt tattcttgaa
    10201 aataaaattg aaagcccgga gcagctgaat gagattgaaa aagaagcaaa gaatgaagcg
    10261 aaggtggcca gaaatgaagc ttggacagat tttatgacgg gaatcaatgc cgaacaaaag
    10321 gaagctgttt cgttactaaa aaaatccgca acccaaaatc aggaaataaa ttcactcatt
    10381 gatgaattag aaaaaacaat taatccaatc cgggctgatg ccataagggt tgtaaaaaaa
    10441 gcaatcagaa ttctgcgcac tgagcataat gatgaaagaa ataatcttgt caaatggatt
    10501 ctcaagcagg aaaagaaaaa ttatgaaaga tttagttctc atctgtatag tgaatctgcc
    10561 gaatctgccc tgactattgt aaaagttgat ccggtgtatg atagtgaacc cgttctggtt
    10621 gatgggaggg aaatactcca ggctaatttt gataaaattt ttgaaatgga cccccgggtg
    10681 ctggctattg gtgaagatgt tggcaagata ggagatgtga atcagggaat ggcggggctt
    10741 caggaaaaat atggtgaatt gcggttgaca gatacaagta taagagagtg caccatcata
    10801 gggcagggaa ttggctgtgc actaagaggt cttaagccaa tagcagaaat tcaatatctg
    10861 gattacttgc tttatgcaat ccagattctt tccgatgacc tggcaactct tcagtataga
    10921 acaaaaggag gtcaaaaagc accaatgata attaggaccc gggggcatcg attggaagga
    10981 gtatggcact ccggttcgcc catgggaatg ataatcaata gcctcagagg catatatgtg
    11041 ttagtgccaa gaaatatgac tcaggcggca gggttttata atacaatcct ggcatcggat
    11101 gatccggcat taattgttga accactcaat ggatacaggc tgaaagaaaa actgccggtt
    11161 aatcctggtg aatttaaaac acccctgggg atcccggaaa tattaagaga aggaagtgat
    11221 gttacagtgg taacatacgg atcaatgtgc agaattgtaa tggatgctgc gagccaactc
    11281 agtgacacag gaattgaatg cgaagttata gatgtacaat cattgctgcc ctttgatata
    11341 aatcattcga tagttgaatc cttaaagaaa acaaaccgga taatttttat ggatgaagat
    11401 gttgagggtg gaaccactgc ctacatgatg cagcaggtgt tagaggtaca aggcggctat
    11461 caatttttag attcacttcc agctacatta acttctcgcc cccacagacc ggcatacgcg
    11521 tctgacggtg actatttctc aaaaccgaat tcagaagatg tatttgacaa agtatattcg
    11581 atgatagcag aggcaaatcc ttcgaaattt ccagacttat ataattaatt ttctttaata
    11641 tcctgaaggg tatattcagg agtctctaca acattactgg tatgaaaccc acgatcctta
    11701 attcttattc taaatttgaa aaccttattt ctcaatagca ctggaaatcc cagtgaaagc
    11761 atcctgtagg ttagatttcc ctcaataggg gattctttcc tcaaatcaat tccgaggtct
    11821 gcaggttcaa agcgtccatt aaaactctga ccgcaatcag gatatcccag ataagattcc
    11881 cagggaaacc tggtaaatgt cccatcagaa ttcttaatca ggaagtcaat caggaaattg
    11941 taatgatttt catttgctac aaagtacacc gtatccatta cggttatatt attaattgtt
    12001 ggattaataa tatagttagt gcacttataa ggttcttcat agggaggaag gctttcaaag
    12061 cccggccggg ttcgatggcc atacgtaacc agttcctggt cgccatccaa aaaataaccg
    12121 actgcagcaa aaggtccaat gttctcactg gagaagattc ccaaatcccc ttgttgatcc
    12181 tgaaaatgga tggttatatt aatggaatcc tgattttgca aacccatatc ccaatattca
    12241 gctttaacaa attcaatttt aggaaccgta ggaaattcag ttgaactggt gcatccggct
    12301 aaaattacta ataagatgaa aatcgaaatt cttaccatca tcaattacta tcctgcaaat
    12361 ttataaataa ggtttaattt tattgaaatg ttcaggattg attcaaaaac cttacaatca
    12421 ttatcgtcaa ttacctttga gaagttggca tttgacatat tcaaatatca gtccaaaaat
    12481 aatccggttt atgccaaata cctgaaactg gtggagatta aaccgggtaa tatcacaggg
    12541 ctaaaccaaa ttccctttct cccaattgaa cttttcaaaa caaatatcgt aaagaccgga
    12601 aattggaaag aagaaactat ctttcaaagt accggtactt ctggtcagcc cagtatccat
    12661 tacgtaaggg atctttcctt ttatcaggaa ttagccgctt tcaatttcca gcgattttat
    12721 ggtaaactga atgattatga tatttatgca ttattgcctg gctatgtggg gtcaacgaac
    12781 aattctttaa ttgccatgat ttctcatttc attgagaaat caggttcact caaatcagga
    12841 ttttttctta cgagccatgg aaaactggtc agattactga gctcaaattc agagggtaaa
    12901 aagaggttac tgataggtgt ttcccatgcc ctccttgact ttgctttaag gaatcctgtg
    12961 aaattgaaga ataccattat tatggaaaca ggagggatga aaggacgaaa aaaagaaatc
    13021 acaaggaagg aattgcatga cattctgaaa agtaaactgg acgttggaaa aatccattct
    13081 gagtatggca tggcagaatt aacttcacaa ggatattcta ttggggatgg ttactttgaa
    13141 tttccaaact ggatgagaat tatcattcgc gacataacaa acccattcca aagggttaca
    13201 aatgggaaag ccggaggtat aaatgtcatt gatctttcaa attatcaaac gtgttcattt
    13261 attgaaacaa aggacctggg acgaatgaaa aatgaaaaaa aagtagaaat tttgggaaga
    13321 tttgacaatt cggatgtccg tggatgtaat ttattagtga gataaccgtt actattttgt
    13381 ttgcgggttt tttataattt gagtatgttt ggtcaaatta ggctagtttc acataacctc
    13441 gaaccatgaa gagtagatta attattgttg caatattgat tatgggggcc gtagcgtgta
    13501 cgcaaaagat ttgccccaca tatgctaaaa ctgacgtaaa agcgtctgaa aagcaccaaa
    13561 atctataatc aggcatattt cttaatgtca ggttcatcaa ttgtgtctcc acacatgatg
    13621 atgagtcttt ccgtcacatt tctcaattca cgaatattac cagtccattt cattgatttc
    13681 agagccttga gcgcattatc agtaattttc tttggcttga taccatattc cgcagtgatt
    13741 tctaccagaa agtgctgaac cagcaggggg atatcttcaa ttctttccgt aagacccggc
    13801 accggaataa ttattacaca taatctgtga taaagatctt ccctgaattt cttctcttca
    13861 attaattgtt taagatcctg attggttgcg gcaattaccc tgacatccac tttgatgtcc
    13921 ttatcacctc caacccgggt tattctgttt tcctggaggg cccgtaacac tttagcctga
    13981 gcagaaaggc tcatatctcc gatttcatca aggaataaag aaccaccgtg agcttgttca
    14041 aactttccaa tcctttgttt tactgccgag gtaaaagatc ctttttcgtg accaaataat
    14101 tcactctcta ttaattcgga tggaattgca gcacaattca cttcgatcag aggtccttca
    14161 ctcctttgac ttttttcatg aagccatcgg gcaacgagtt cttttccgga accatttggg
    14221 cctgtaatca gaacgcgggc gtcggtggga gcaacttttt caatagtttc ctttacttcc
    14281 tgaattgatt cggattcgcc aacaatatca taggctttat tgatcttccg ctttaagact
    14341 ttggtttgcg aaaccagagt tgatttatca agggcatttc taatggttaa taaaagcctg
    14401 tttagatcgg gaggtttttg aacaaagtca aatgccccaa gcttcgttgc ttcaacagct
    14461 gtttcaatgg tcccatgagc tgataccata ataaattgag tatccaggtt gaattctttt
    14521 gctttttgaa gtacttccat tccatcaaga tgaggcattt ttatgtcaca aagcacagcg
    14581 tcgtaaccat tgcggattag cttgtccaac ccttcctgcc cgtctttggc ttcatcaacc
    14641 tcgaattttt catattcaag aatttctctg agagtatccc ggatactttt ctcatcatct
    14701 agaatcagaa ttttagccat aataccaaat taaaaaaaga tgcaagtcta taagccgggt
    14761 tctgtatcca tcaaaatgga ctcctatcat ttatcttgac ttgatgtcac catcaagttc
    14821 cagcgaccca cccgtttcga gatcaactga tctgaggagg tactcatatt ctcgcgaata
    14881 tcgaaactgc ttggtctttc agctcacaag ggttaccaag ccccgcaagt taccttttgg
    14941 gcggtgagct cttacctcac cttttcaccc ttaccccatt aggggcggtt tgttttctgt
    15001 ggccctgtct gtcatcgaca atgtcgatgc cttcctgtta ggaagtgtga ttacctctgc
    15061 tgcccggact ttcctcttta ctaaaaaata aagcgatagg acaacttgca tcaatacaaa
    15121 aatagcgaat tccgggcaga atagaattca aaattttcag agtagtttca aaacgtcaaa
    15181 tatctcacct taactccatt tttgttaaac tcaaggccag gggatggcaa atgaatcatg
    15241 ttaataaagt aaaggataga attaagaaag gctgaccggg tatgaatgtg ggaaaggtca
    15301 atgtcaaggg acaggtaata ttgccgatag gaattgtacc cggcattgta attctcagat
    15361 ttcctggcgt aaatcatgtt tgttgagcca tatccggtgg cgatattcag ccatttcgga
    15421 aatctgaatt tattttcaaa aaatttattc aggtcgaatg atagccaata cgtttgacca
    15481 ttataatctt tgaaaagctg ttccataaaa ttacctccta agacctgagg cctcgtgtcg
    15541 gcaaaaccgg ttggagaaaa tgaaaactta ggatgaattc ttatctcatc ccaaaccaat
    15601 ttttggccaa gatataatcc aattcctccg gcatttgcca aaacatccca accggaagca
    15661 ccaaattctg aagaataccc atcaagtatt tcaatagtag acataaaagc aaaactagtt
    15721 gcagatccat agagatcaga tttcatatca ttaaacccac accactggta tagtctggaa
    15781 gctattgcat tgaaatggaa accggaataa agatgtcctg ctttgtcaac ctgattccac
    15841 tcttttccgt catcaaaaaa gtgaaaattt tcccggggtt gattttgata ccataattca
    15901 ttaaagagga ctatcgaact tccgtgcaaa acaggaaggc cgaccagtaa tgcagtagtt
    15961 ctttttttgt ttaatgaatc accatgtcct gatcccgaac ctggaaatgg aattatcaag
    16021 ataagtaaaa atgccccaca aagaattcta gttaagcggg tcatcttcat tttttcgtgc
    16081 ggatgtatcc gcaggatccc gcgattcaat ttctttgagt cttcttttgc ggcttgcatt
    16141 gaaaatgtct ctcacctcat tgaaactttg cgtatgaatt aaactcactc cagcggtaaa
    16201 gtttgaggtt tggttaaacg tggtcagtgc ctggtttgta tttgtccggt taaacatctt
    16261 tactctgaat ttcccctcgg gagtaagtga atactccaca gtccagtcgc cggcgatgct
    16321 ggcaaaatca gccttatttt cagcatctgt aaaaccacca tcgcgggtaa ctctaaggcg
    16381 gccctcaaga aacgtgtagg ataatcttaa ttgaaaagca ttgaagccct cctggtctaa
    16441 ttgaccaagg tcaacatcta tatcgaggtt ttcatctaac tgagtgatcc aataacttaa
    16501 ttggtttgat aaaaactcac taactgagtt aactatgtaa ttgctgcccg aaagcaatcc
    16561 ctcctgttct gaaaacctcc ggaggataag gagactgaaa acctgtcttt tcatctcttg
    16621 ttcatcggaa gcaattctgt tttcaaatga agaaaccatg gaggccaggg aaaatgactg
    16681 cccattgtct aaggtgatga tatcaggata gctggtcacc ttaatatcga acgcgatatt
    16741 cggtgacagc aggggtcctg caaggttaag cagcacctca gtgggatacc tgcgatttga
    16801 ttccgggcta tgattataaa ccgttgtatc catcaaaggc ccaagggagg tctgttgcat
    16861 ataagatgcc tgaagatcaa gaattccccg atatggatct cctgcccatg atattgaact
    16921 gcccggtaga attgaaaatt ccttgttgat tatattatat agggtgaaat tatagccccc
    16981 ggaaataaac tcataatcgc caatcatggc aaaatcaccc tgagtatcga tttgaagctt
    17041 aattttcccg tttcctcgtc cgcgaattat atctcccgat tgaatatcaa aaatcatttc
    17101 tgcataggca tcaggggtga tttccagatc gaaatcaagt aataatcctt tgagattaac
    17161 tgttttttct tctccctgaa tagaatcgat ttcacccgaa gaaaaattca caaaagttat
    17221 ataatccctg gcttcaatac tttcagtatc gccaagtggg ataaataacc gtgttcctct
    17281 gtttgtacgg gcattggccg aaattttcaa tttacttagc ggcccattga aactaacctt
    17341 tccggtaact ttcgcatttc cgtaaaaaag atcgttatcc tttgaggtag tattcaaaac
    17401 ctgaaagttg tccagattgc cgctcaaatt cagggaaaaa tttctgaacc ggttgtgggt
    17461 aaattctcct gacagaaaag aattgttatt atcgatgtct gttacctgta tattctgaaa
    17521 tctaattttt gattctgtga aagctatgtc tccggccagg ttataattgg tgtttagata
    17581 atttaccata agcgacccat cgtttacgat tccggctcca ttaatataag ggaacagagg
    17641 agttccggta atagaaaatt cgcccgaaat agttcctgaa atatttgaga agatggtttc
    17701 aatgaagggc tcaataattt tgagatttgc ttcagaaaac ttagccttga gattaagaga
    17761 attttcactt tcaaagggtt caaataatcc cgttacatca attatatcct tttcctcccg
    17821 gtcgacttta aaatcaattt caaataactc agattgtgga atccattcac ttcttccctg
    17881 aatattgcca accagaaaat catttattct cattgaaagt atttctaccc tggaatcaat
    17941 caagggatga tggtacaaat cgcttaatct gaccccacca ttaattatcc ctgtaagatt
    18001 ccggttaatt aatggattaa tattctccat tctgacgctg tcaattataa ctgtcagcat
    18061 tttgtctgcc ttttcagaaa tatccccatt taattcgatt gattgatttt tattgaaaaa
    18121 cctaagatct ttaactgata tttctctacc gctgctggca atcaggttat cattgtctat
    18181 tctccaattt tcatttaacg ctaaaatatc agaaggttta atatgaatgg ttgtgctatc
    18241 ttttaagaaa ttcactgttc cgaatatgtc ggcatagttc aggttatttt catgagcaat
    18301 tgtggcatta aaatccattt cattattgct ccagacacag tctaatgtca tatcttttgt
    18361 tcgcgtcaat tcgtttatta attggttttt tgaatagacg taaagcattg ccaggatttg
    18421 agtactgtca cttattttgg atgctgagag atcaatttca ttatcaaaga atgatgactt
    18481 ctgataattt acggtatcaa ttgatgttga aagactgaat atggaagtaa accctgaact
    18541 aaatcgaccc tggatttggg aattttttga aaccgaaatc tctggaacca ggaattgtaa
    18601 caaggggttg atatctttta gattaatgaa ataatcaacg taataatcat ttttagaaat
    18661 ctgctctgat ttaaacctat agtattgact caagctatca ctctggtttt taaaacttaa
    18721 ctgaaactcc ttaaccagag ccgtaatgtc tctaagtgca gaggtatagt taaaatcacc
    18781 ctgaaggtca acagaaaaat ctgatgcatt tacctttagc aatctgaatg ctgaatttcg
    18841 ttgtgagaac aaatttaacg agtccaggtt aagctgtcct tccggattta tcaacattgt
    18901 gttcttcgta attatacgtc cctgtaagct atcaacctga agtcctgtca cattcacatc
    18961 aaattctgtt cgcaaaaata agtcagacgg aagtaaacca acaggttcaa aaaacaaagt
    19021 atcaattcta ccatgaatct taatttcatt tttcttttcc cttaaatcaa ttgagcctgt
    19081 agcattaaat cttaggtttg gatcagaaat tcgaatattt ccatcaaaga attctttagc
    19141 aaaattagca ttggttacga tatccgaata attgtaaccg tttattccaa tctcggaaat
    19201 cactccatca agtttaaatg cagcagtgga caatgttacc ccgcgaccgt caatggaacc
    19261 cttcagagag actttctgca aaatattatc ctggaaaaat tcaccgatat caaaatcagt
    19321 caactcaaga ttacctttgt aagaaattat ttctttggaa tctttcctgg gtatttttaa
    19381 attaaggtcg cagtctatct gcccgaggtc actttcaaaa ttcccgcgcg ccagaaaatc
    19441 gtgaatattt ccattaagac ttccaaaaaa gaaaaggtca tttaatttac taatcctttg
    19501 gtagacattt tccggcacat aatcttttaa atctgaaatc aaaattcttg atttcgaaat
    19561 ggtcgcatca aagtaaaaat cattaagttc aggtaatcca tttaagatga agtctccttt
    19621 tatgagactt tgattgccca atgctaaatc caagtccttt attttcaaat ctccaatctt
    19681 accattcaca gatcctgata atgtgaaaac ttcgctataa ccggaaaaag ccggaacaaa
    19741 taatgaaagt tcttcagaag caataacaca acttttaagg gtagttttta tgttaacatt
    19801 tgggacgaaa tcactcaggc tttgatatcc gtcaaaatta aaaatgattt catcagaaat
    19861 ataacttttc ccaattgcag cctgcaattc tgtcaacttt aaccgcgacg gatgaatgct
    19921 aagaagacca gtgattgaat gaatctcagt tttgttttga atgttgaagc ctgataaact
    19981 tttgatatcc atcgtaatgg tatctttaag gatggtaaga ttatcaacct gggatttaac
    20041 gttatttata gtaaaatgat ttatatcgaa ataattttca tttacaggtt ttgacgcatc
    20101 ccataatgta taagtggtgg agaaaagctt tacagttcta ataacaaaag acctggtact
    20161 tgcaccttct tttttgtaat gggcaactat agtctgaata tattcctgga tattgaatct
    20221 ggctgattca ggatttcgaa acattttgaa gtctgcattg aacaatacga tctcatcaat
    20281 tacaatttca tatgtagaag ttatattgga aatagtgaaa tcaattactg cttcatcagc
    20341 cttgaacatt gagctgtcct gaaggtcttt aactgatatc ccttctaccg caatcttatc
    20401 aaaccagttt atactaacgt aatctactgt ggtaataaat cctgtagttt ttgtaagcat
    20461 ttcgccaagt ctttgcacta ctttggtttg tacataaggt atctgtaaag cagaagcaac
    20521 tcccaccaga aggatcaaag gggttccaag aacccaaaac actattttag caattcgttt
    20581 tttaataact tgcgaccggt taaatcagaa attatgcatc ccgcgatact tgccattgaa
    20641 tcctcttgcg atgaaaccgc ggcatccata atagttgacg gcaaagttct taataatata
    20701 gttgcaacgc aatttgtcca caaaaattat ggaggagtgg ttcctgaact tgcatcccgg
    20761 gaacatcaaa agaatatcgt gccagtagtg gatgaagcat tgaaaaaagc agcaattaat
    20821 atttctgatt tggatgcagt ggcattcacc cagggccccg gattattagg ttccctcctg
    20881 gtaggcgcca gctttgccaa gtcaattgca atggtgaatt ccattccctt gatctctgtc
    20941 caccacatgc aggctcatat tctgtcacat tttattgata agcctcaccc cgcgtttcca
    21001 tttctttgct taactatcag tggtggccac actcaaataa tacttgtaat tgattacctg
    21061 ataatgaaag ttttgggaga aacccgggat gatgctgtgg gagaagcttt tgacaaagga
    21121 gccaaaattc tgggacttcc gtatccggga ggtcccttga ttgataaata ttcgaaaaaa
    21181 ggtgatgaga acttcgtgga tttccctgtt accgaattgg caggacttga tttttcattc
    21241 agcggaataa agacttctct tttatattat ttgagagatc agcagaagaa aaataggaat
    21301 tttattaatg atcatcttaa tgatatttgt gcaagttacc agaaagcatt ggtaaaaatg
    21361 ttattgataa aattagaaca agccagtaaa gaaaccggaa taaaagaact agccatagcc
    21421 ggtggggtat ctgttaactc tttcctcaga aattctcttg atgaaaagtc aaaagaaatg
    21481 aactggaatg ttcattaccc taaatttgaa tactgtactg ataacgcggc tatggttgct
    21541 atgactgctc attataaata tatcaacaat caatttgaaa aggctgatgt gaaagttgat
    21601 ccacgattaa aatttcctga aaatgaaatc aatttttaaa ccaggagact ccaggaagct
    21661 tgaaataaaa gttagccggg atgatcttgc aaaattcaat ggaattgtag tgcatgaagt
    21721 ttgttctact tttgcacttg caagaaatat tgaatgggca acacggcaat atgtgttgga
    21781 gatcactgat gatgatgaag aaggaatagg aacttctatt tccattgacc atgttggtcc
    21841 tgcgtttgag ggggaagttc tggagattga gagtgaggta aaagatttta acgggcatga
    21901 attgatttgc gcataccagg taaaggtaaa gaacaggatt attgcaactg gaactaccgg
    21961 ccaaaagatt ctaaaaaagg aaagaattga aaaaatcttt agtagtttac cgggataatg
    22021 gctaaggaaa aaagattttc cggtactgta aatgttaaga ataaaagggc ttcatttgaa
    22081 tatgaattcc ttgaaaaatt tattgcaggc attgctttaa caggaacaga aattaaatcg
    22141 ataagagaag ggaaagttta cctgcaggat ggattttgcg ttttccataa tcatgaattg
    22201 tatttgcatc aggttactat ctccccttac aaacagggcg gacactacaa tcatattggg
    22261 gatcgggaga gaaaattact tttgaaaaag aaagaactta aaaaattaaa aagtaaactg
    22321 gaagaaaaag gtctttctat aatcccaacc cggttgttca taaatgaaag ggggctggca
    22381 aaaattgaga ttgctcttgg tcgtggaaaa aaattatatg ataagcggga aagtataaag
    22441 gaaaaagatc ttaaaagaga tatggaaaga cttaaactgt aattatggaa tttcttgaaa
    22501 gaggaatttg cagattagga attgtgcctg tcagatctgc tcccggggat acctgcgaaa
    22561 tggttaacca attattgttt ggtgaacact acactgttct ggaactttca gacagtaaag
    22621 agtggattag gattaaagct tattttgatg agcatgaagg ttggattaat agcagacaac
    22681 atcatcggat aacggaagat tacttcaacc aaattaataa ttcagattat aaaataagtc
    22741 tcgacataag ctcaagtata ttataccgga agaatactat ccacatagtc atgggcagca
    22801 tccttcccat ctctaccaac gagttattta agatggaaga aaagctggct tttaacgggg
    22861 aatccaaaag tcttggtcag aaaagagagt ttgaatttct taaacaggtt gccggaaaat
    22921 atctgaactc accttatttg tggggaggta aatcgccctt tggcatagac tgcagcggat
    22981 tcgttcagca ggttttcaag gtttgcggtt acaaattgcc cagggattcc caccggcaag
    23041 cagaaattgg gacgaaaatt gatgaattac cggatgtttt accgggagat atcgcttttt
    23101 ttaagaatag taaagggaaa attgctcatg taggtataat tttagaagat ggaaacatca
    23161 ttcatgcatc aggacaagtt agaattgaca aattgacgga gcatggaatt gtggatgaaa
    23221 aattgaaaca attaacacac aagctgtttt ctattaaaag aattttgaag actttatgaa
    23281 taaaagagta cttgtattga atcttgatca cagcccgata tcggtttgta ccgtgcaaag
    23341 ggcgttcctg ctggtatatt tgaataaagc acagtcgctt atgcatacca aaggctttta
    23401 tttacgatcg gtaagcatga gctatccaat gccagctgtc atcaaactaa acaactatgt
    23461 tcaaatccct tatatggggg tcgaattaac ccgtcagaat atcttcaaga gggatagttt
    23521 tacctgtcaa tattgcggtc atcaaaaagg tttaaccctt gaccatgtcg tacccagatc
    23581 caaaggggga aaaaccagct ggacaaattt ggttaccgct tgtaagagat gcaatacgca
    23641 aaaaggagat tacaccccgg aagaggcggg tttgaattta atcaacagac ctttcaggcc
    23701 atcctacatt atgtttttaa gagacttctc aggttttgtg caagaagagt gggaaccata
    23761 cctggccact cacatggcta gttaaatcgt agggtgaagg tagttctgct accgggagtt
    23821 gattcgactg aaatggttcc attatgcaac tgcatgattt gtcgcgataa tgcaagtccg
    23881 atgcccgagc cggctttctt agtcgtatag aacggcacga aaatccgttc caatgcttcc
    23941 ggaataatgc cttcaccatt gtcggtaatt tcaatggttg ctttcagctc aaaatcaaca
    24001 gaacctttca ggctaatcct tgggttcttc actttaccag ccgcctcaat ggaattcttc
    24061 acaagattga tcaataccat ctggataagt tcttcatcgg caaaaataaa caagtcctcc
    24121 ggcttcacct ccatggtaag ttctatattc cccttcctga tttcagctct atgcaagctg
    24181 gcaatttgtt ccaataattc atgaagcaaa attttcttga gtttgggagt tggtatcgtg
    24241 gtataatctc tataagcttc cacaaagcga atcaaccccg tgcttctgct cttaattgta
    24301 tttaatccct cttcaagatc atctatagtt tcttcaagaa tttctatttt attgttccgt
    24361 cgaaccagat cttcaaccag aatctcattc aaagtggagg ttagtgatga aataggagtg
    24421 atggaattca taatttcatg ccttaaaacc ctggttagat tttgccaggc ttcaacttct
    24481 ttttgctgca attcagattg aatgttttgc aaggaaacaa gtttgtagat ttttcctcgc
    24541 agttttagct cggttgcatg gacagcaaga tgcacgttac tattcagccg cagcatccgg
    24601 ttaattcccg gtttcaggtt taccagcatc cggtgaagtt ccggttgttt gtcttttaat
    24661 tcttcaatat ttcttaactg cggtgaaccg aaaaaatgct ttgtcctggc atttataagt
    24721 tctacatttc cttcttcatc aaatgaaagc aatcctgtag ttacatgctg aactactgta
    24781 ttcaggtact gccagtgttc ttccttttca cctctggcta cacggaaggc ttccaggacg
    24841 gcattaaatg cctggttcaa gtctgcaaag ctctgcccca gtttattatc agaggtaaag
    24901 cctgaaataa aatcagaata cctcaccgat tcaagaaacc tggttaattt tctgttagtc
    24961 ttttccacat acctgaaaat ttcaaccaga atcagaattg aaatccctat caatattatt
    25021 gcactgacaa tttttgaagg gtcgttaatc agtaacgcga ctcccgaaac acataatccc
    25081 agtaaaatta cccgaatgaa aacaccaatt cgaaaccggt taaagcccat acttttctag
    25141 ccttctataa agagaggatc ttgttaagcc caattcagta gctgcctgag taatattacc
    25201 attatatttt ttcaatactt ttctaatcaa aactttttca acttcttcaa ggtgaaagtt
    25261 ttcaatcaga aatgcatctt catccttttc ctgacctttg gagttaaata taaagtcttc
    25321 aggctgcagt accgaagtaa cgctcataat aatggcacgc tcaagggagt gttgcaattc
    25381 cctgatatta cccggccagg aatatttttt cattctcgaa atggcagcgt cacttatttt
    25441 gtttacggat ttattatatt tttttgaata gtagttcagg aaatgattgg ccagaagaac
    25501 aacatcatcg tcacgctcac gaagcggcgg catgttaatt tcaatagtat taattcggta
    25561 aagtaagtct tgccggaact tgttttcctt taccatatca tagatgggca agttagtggc
    25621 acaaaccaga cgaatatcta tgggaataat tttatttgat ccgaccctgg taatagtacg
    25681 attttgaatc gcggtaagaa gttttgcctg caaggggagc gacaaattgc caatttcatc
    25741 caggaataaa gtcccgccgg atgcaacttc aaatcggccg gctctatctt cctttgcatc
    25801 cgtaaaagag cctttcatgt gtccgaaaag ttcgctttcg aaaagtgttt ccgtaatgga
    25861 acctaaatca acattgataa agacttcatt agccctgtgg ctattccggt ggattgccct
    25921 tgcaaccaat tccttgccgg tcccattttc acccaaaatg agtacgttgg catctgtctt
    25981 tgcaaccctg tctatggttt cataaatttt ttccatcaca aggctctgcc cgatgatttc
    26041 caggtatttg ttgttcagat cttcattcaa atgcttttgc ttggtcctga gatctttgat
    26101 ttcaagtttg ctttctctca atttcatggc tgaatgaaga gtggccagaa gcttttgatt
    26161 atcccagggt ttcaaaacaa aatctgttgc ccccgctttt atcgcctgta cagcagtatc
    26221 tacatcgcca tatgcggtta ttaataccac cacagcggaa ggatcgattt ccagaatcct
    26281 ctccaaccaa taataacctt ctttgccgct gctcacatct ttggtaaaat tcatatccaa
    26341 tagaataatg tcgtaagatt cattattaag cactgttggc agcgcttcag gacttttctc
    26401 cagatcaacc tgcaaaaaat gtcgcttcaa aaagatcttg gctgctttta gcaggtcttc
    26461 gttatcatca acgattagga ttttgcctga tttattttgt gtattgttca tggtgtcatt
    26521 tctggagtac cttacactat tacaaagtta ataccgatgt tcgattccgg actataaatc
    26581 ctcttgtttc acaattttag ccgggttttt cgctttgcca ggatcaatca aactggcagg
    26641 aacttagata ttaccgtccg cttgcggaca cctccgttcg tgcacgaacg cgggaaaaac
    26701 taacaaccta tgaaaaacgt agaataaaga ggattcaatc aattggcaca ctattggcaa
    26761 aactgtaatt acctatttaa gtgacaattg aaataaaaac tcattattga tggacaaaaa
    26821 aattaaaaag aagatatgga cattgaagag gttatcgctg attgccttcg gagtcctctt
    26881 cttttccttc attctttata actttatttg ggcagaccgt cgatcgaaac tcaatgttaa
    26941 ccgcgagaaa attactattg ccacggttga caggggtcct tttcaggagt acataccaca
    27001 aacaggtaca gtctttccca aggaaacctt ttacctcgat gccattgagg gtggaacgat
    27061 aaagcgtgtg gtgaaagaaa gcggcgcgaa tgtgaacccg ggagatgtta tcgttgaact
    27121 ctctaattta aatcgtgaac ttacggtgat gagccaggaa gcttcctata atgaaagtat
    27181 caaccgggtt cggcaaacaa gactgggtgt gacccaaaat gatctccagc aaaaagcggc
    27241 attggcccaa atagaaaacc agttggctat tctaaagccc cagtatgagc gggaaaaaat
    27301 actatttgag aaaggcttga ttgcccagca gcaatttgaa agaacaaaag ctgattatga
    27361 atataacgta aaacgtcttg aatttacata tacttcatat agagaagata gtgtggccag
    27421 aatccgtcag ctgcgtgagt tggacgattc ggagcgaaga atgaaacaaa gccttgaagg
    27481 agtaggtaaa attcttgata acctgtttat ccgttctcct attgaaggcc aactgaccac
    27541 gccactgctg gtagtgggtc agtcaataaa tcagggtcaa cgcattggtt cagtggatat
    27601 tgaaggcagc tataaagttc aatgtcttat cgatgaattg tatctgccta aaatttctac
    27661 aggcttaact gcaacctttg actttaataa ccagtcttat tcattagaaa ttggatatat
    27721 ataccctggt gttgatgctg gaaggtttag cgttgacatg tttttcgcag gtgagcagcc
    27781 ggcaggaatc cggaggggtc aatctctgag actgaaaata gccctgggag gttcttccga
    27841 agagttactg ttgcctgtcg gtggtttcta taaagataca ggcggaaact gggtatacgt
    27901 attggaggat ggtggtaagg cggtgaagcg ggatgtccgt ttaggtagga gaaacacaga
    27961 attcttcgaa gtacttgaag gacttgaccc cggtgataga gtaataactt ccagctacga
    28021 taattttgga aacaatgaag tactaattct aaattaacaa ccataaagac tttcaacaat
    28081 gagtaacacc atgatcaaaa tcaaaggact aacaaagttg tacaccaccg atgaagtgga
    28141 aacgacagcc cttaacagca tagacattga gatccaggaa ggagaatttg tcgccgttat
    28201 gggaccatcc ggatgcggaa aatcaacctt actgaacatt ttaggattat tggataatcc
    28261 taccgatggt gagtattggt ttggtgagca tgaagtttca aaattcagtg aaaggcagag
    28321 agcccaactg agaaaaggat ccatcggatt cgtatttcag agctttaacc tgattgatga
    28381 attgaccgtt tttgaaaacg tagaacttcc tttgctttac cttaaagtac catcaaaagt
    28441 aagaaaagaa aaggtggaag aagtgctgga acgcatgaac atcatgcatc gaagaaatca
    28501 ctttcctcaa caattatccg gaggtcagca gcaaagggtc gcaattgcca gggcaatcgt
    28561 ggcaagaccc aaattaatat tggcggatga acccaccggt aacctcgatt caactaatgg
    28621 tgaagaggtt atgaaattgc ttgcccagct aaacgaggaa ggaactacta ttatcatggt
    28681 aactcactca ccttatgatg caaattacgc tcatagaatc atcaacttgt ttgatggtaa
    28741 agttgtgact gagaatatca aagagcaatt ccacatttaa tataattata ccaaaggggc
    28801 aatcgaaaga ttgtccctgg ttttttagcc tgaccaaccc aaattttaca gagttaatgc
    28861 aacttcaata actctggtat tgtcttatct taaaaacact accatgctga aaaactacat
    28921 taagattacc atcaggagct taatgaagag caaaatcttt gtcttcatta atattattgg
    28981 gatggggctg gccctggcgt gctgtgttgt agcgtatttg aattataaat acgcgtatgg
    29041 gtttgattcc gatcaattga accggaagga gatataccgg attgatttta cccgtgtatt
    29101 tgaaggacga tcaagagact ggggaattac ccccctgcct cttggaaccg caattaaaga
    29161 aaatataccg gagctggaag aggttgtgcg atatcacccg actggaggca atattcgtat
    29221 tgaggaagat ctgttcaata cgaacatcac ttatgttgat gaaaaattct ttgaagtctt
    29281 ttctttccca ttgaaatacg gtaatagtaa cgaattatca gatccttcaa agattttcat
    29341 ttcagaaaat ctggcagcaa aatattttgg gaatgaagat ccagtaggcc ggcagataac
    29401 tcaagtcctt gatagcggca aacgagaata tgtagttgca ggcgtaattg ccaggatgca
    29461 aagcaattca agttttactt ttgtggaatc aattactaat tgggctaact acaagggaac
    29521 gtatccaaat cttgatgaaa ataactgggg agcctggagc actgtctttc ttaaaattag
    29581 cgatccatca agaattaatg cgatagaagg acagcttcag cagtacgtag aaccgcaaaa
    29641 cctggcaagg gaggatttca aagtaaccag ttactacatt gaaccttttg atggcatggc
    29701 agaaaggacc agggaaaaag aaatggatag ctataccagg gagggtttgc ctacacctgc
    29761 agcaattgta cctgcaataa tggctgtcct gattcttttg attgcctgct ttaattttac
    29821 caacacgagc atagccattt caggccgtag attgaaagaa atcggactta gaaaagtaat
    29881 gggagggagg agaaaacaat tggttattca gtttcttgct gaaaatatag ccttatgcct
    29941 aatcgcaatg attgcaggct tgctcatggc cgaatacctg gttccaaaat attcgcagat
    30001 gtgggagttt ctggatatcg aattaagtta tgtcaacaat gttggattct tcggatttct
    30061 tttcgtgctt ttgctttttg caggacttgt tgccggaagt taccctgcgt tttatataag
    30121 cagatttgat ccaacccgga tattaaaagg aaccgcgaag tttggaggcg cgggagcaat
    30181 tccaaagcta ttgttgggct tgcaatatgt tatatccatg cttgcaatta ttatgggtat
    30241 cgcgtttgtt caaaatgcca gatatcagga agaattagat ttgggatttg acaaggctgg
    30301 agtgatatat acttacgtga acaattttaa tgaatttgag atttatagaa atgctttagc
    30361 tgaaaatcct gatattgaag ttatcgctgg atcggaatat caactttaca gcagtggaag
    30421 aaatgatccc gtaagatatg aagacaaaca aattgaaact gatattttac atgtgggtga
    30481 cgattacctg aaggccatga gcatagatat tatcgaaggg aggtccttcg ctgaagattc
    30541 cgaaaccgat cgtcttgaat caattctggt atccccttcc tttataagcc aaattggctg
    30601 gacagataat ccgataggaa agagaattgt gtgggcagac acgatttctc tgtacgtgat
    30661 tggtgtggtt aaggatattt ataccaacgg attgtgggat gaacttgatc ccatgattat
    30721 ccggtatgtg ccggaaaagg attacagatt ccttacggta aagacctccg cgatgaaaat
    30781 cctggaagta aatgagttta tgaaagacaa atggaatgaa atttttccaa cgcgtttata
    30841 ttcaggccgc agccttgatg aagtattgag agaatctgct gaggtaaatg aaaatataat
    30901 aaaaatgttt ttgttcctgg gcattattgc taccatactt tccgcaaccg gcctgtttac
    30961 tctggtttca ctaactatta taaggcggat gaaggaaata ggagtgagga aagtgcttgg
    31021 cgcctcagta gtaaacatag cgaaaaatct gaatcgtcag tttttcataa ttttagtgat
    31081 tgcagcaatt ttgggttcag tgttgggcag ctacatggga tcaatgttaa tggattccat
    31141 ttggaaatac tatgtagcaa tgaatattcc atccgcgatc atatctgttc ttttgatgct
    31201 tttaatctcg ttaataacag ttggctttaa agtatataac gccgcaatgc tcaatccggt
    31261 taatacgctc cgatcagaat aaaaaactgg ccttgtattg cagaaggtat tatattgaag
    31321 tcgaaacctt tcaaaaagta gaagtatgat caagaattac ctgtctatag ccttccggaa
    31381 tatcaggaga aattcactgt attcaatcat caacattctg ggattatcag tagggatagc
    31441 ctgttgcctg cttattctca gttacgttcg gtatgaactg agctttgatc tgcctcacaa
    31501 aaaaaatgac cggatatacc gggtctacat ggatgcaaat tttggcgggc agcaagccct
    31561 tatcggcgta acacctaata ttatcgctcc catccttcag tctcaatttc ctgaggtcga
    31621 aaaagtagta agggtctaca atattggaaa attcaggcca caaatagtga aagttaaaga
    31681 ggagctctat gaggagaaaa atttctatta tgccgattct tccatatttg agatattcac
    31741 ttttcaaccc gtttatggcc aactggacgg ttctcttgac aagccaaaca ccctggtgct
    31801 tacacagtca gcttcacaaa agtattttgg aaataaaaat cctgttggct ctgttgttaa
    31861 tgttaacggc aacacagatt atgaaataac cgcagtaata gaagatatgc ccaacaatgt
    31921 tcacttcaga tttgatatgg taggttcttt tcagggctta agctgggcta agccggaaaa
    31981 cctgactttc ggttcagcaa acttttatac ctacgttttg ctttaccccg gagctgacca
    32041 tcaaaacttt gaagataatg ttgcgaccta tacggagaat acttacggag gacaatggaa
    32101 acagtctggt tttttcctga ccctgaaaat ggaaccgcta aaggatatct acctgagatc
    32161 accaattgat aatgacattg ataaaaagag cgacatccgg tatgtataca tcttttcttt
    32221 tattgcaatc ctggtgctgg tcatagcctg cattaattac atgaacctgg caacggcaag
    32281 aggttcagaa cgtgccaagg aagtggggat gcgaaaaatg ttaggcgcca gaaaaaatga
    32341 gttaatctct cagtttatgg gagaatcaat tctgataacc ggcttcgcga ttttttccgc
    32401 tatagtgatg gtagaattac taatgcctgt gttcagagaa ctatcaggca ggcaactcca
    32461 aaattctgtt tctgaaccaa tattgatttc ctcgctggct ggaatctgga tcggagttag
    32521 tttactagcc ggcttttatc ccgcggtgat attatcatcc ttcaaacctt caaaagtgtt
    32581 gaaaggaagt ttcaaatctt ccgcggcagg cagaggcctg cgaaaaatat tagtagttct
    32641 tcagtttgcc atctctattt tcctcattgc cggaacaata gtcatcttca ggcaattgga
    32701 tttcattcag aacaagaaat taggatatga caaagaccag gttatagctg tcccaattga
    32761 taacaaaata gctggaaacc tggacatttt caaaaatgaa ttcgtatcaa tacccggtgt
    32821 tattaatgtc acggcggcat ccgaaacgcc ttcggatgtt aaaggagggt attcctatat
    32881 tttcgaagga aggtcattgg atgagtcaat gtcagtgaat gcaatggcag tggatattga
    32941 ttttattaaa acactgggga ttgaagtagt ctccgggagg gactacagcg aagacagcaa
    33001 gcaaaagccg gatttctcct tcatattcaa tgaagcagcg gcaagccgct caggactgag
    33061 tattgaaaac ctgattggtg aacggatttc ccttaacggc agaaatggag tggtgacagg
    33121 gattatgaag gattttcact ttacttctat gtatagagaa attggaccac tgattctatt
    33181 cattgagccc agtcagtttg cctatatgat gataaagatt gacggccaaa atattcagca
    33241 gaccctaaac accctggcca ctaaatggaa gactctggct tcgcatcgcc cgtttgaata
    33301 tgggtttctt gatgatgaat ttacccggtt atacgctgca gaaatgaaag ttggtgaatt
    33361 attttctgtg ttcgctattg ttgcgatttt tattgcatcc ctgggattgc tgggtttggc
    33421 cgcttatatg gcatcacaac gcgccaagga gatttctgtt agaaaagtaa tgggagccag
    33481 cgtaggcagg gttgtattac tcctttcctt cgattttgcc aggcttgtgc tgatagcctt
    33541 cataatctca gcgccattag cctactatat tatgaacaac tggcttaatg aatttgttta
    33601 cagagtctct tttggagtgg agacgctttt aattgcagga ttattggcat ttataatcgc
    33661 ttggggaaca gttgcctggc aggcattcaa ggcagcaaca ataaatcccg ttgatattct
    33721 caggtatgaa taattggaca agtattagta gttttgcgag agtataaagt acttatatct
    33781 gctggatgtt caaaaatcag cttcaatttc atcatccttc agaatctctc aagccagaat
    33841 attattatta tttctaaaca tttccatccc ttaaaaaggt tgaaaatacg ccttgggact
    33901 gatttttagc aattgagggg atattagcca gaaatattta ttttacaatg ataattatct
    33961 atgaaattta ttaatcaatt ttgtatttta cgcctgtaaa gtaaaactac caactaacaa
    34021 ataaattaat aagtaaatga aaccacaatt aactaccagg gaattacaaa tcctaaaact
    34081 gatccttaag cagaaaactt caagagagat tggtgacaaa attggaattt ccagaagaac
    34141 tgtagaaaaa gttcgtgctt ctttattgga gaaaacaaaa tccgtgaatg ctgtaggttt
    34201 agtagcctgg gctattaata acgacattgt ttctgtgagc tccaagaagc cacgaatcag
    34261 actttagtaa gtttagagct taggagagaa aaggaacagg tgaacaggtg acgcaagaca
    34321 agtcctcgcg ctgaaaatct gttctggtac tatttttatg agatcaatat aagagaaata
    34381 ctcttccagt aattctattt cattgaattc agccctgtta ctgagaataa agtgaaattc
    34441 attaaccccc atctccggcg acagcgctgg tctccttcag agataaatat tctttcagaa
    34501 tattttcaaa tttaattttc aattcagtat accggtcaaa agcatcctta ccaggccgct
    34561 ggtctgcacg gttcagcatg gatgataaat aactgatttg atccaggagc attggcgtca
    34621 tgtacgtccc atcggcagta aacatctgat tttcaatctt tgcccatttg tcgtcttcgg
    34681 attttaatga agaattttct ttcaattctt tcgcaatttc cttgcggaca ttttgcactg
    34741 aatttccaac tcttttagcg gtatcctgta aatcacggat tttcagagtt agttcttcct
    34801 gcgacttcag atcatcatag gtaattcctg aagcggccaa gctggggtct atgaccagtt
    34861 ttacggtctg cgtataggta tttccatcaa caatcaaccg aacttgatat tcaccgggtg
    34921 cggccatagg tccgttcagg aaacttcgtc tccggttgct gtcccacgca cctttatgat
    34981 ccatatccca gataaaacga tgggaaccgg cagttttctc caggctggag ggatttcccc
    35041 tgaaaaagaa tccggtggcc atactcggtt catcatcctg actttgccgc ctggtttcac
    35101 tttcaaaact tctgataacc tgttgcctgg catctactat ctccagtgtg atttcatctt
    35161 tcgcattatg agcaagataa taatcaatta cgacgccagg acttggatat tccggcacgg
    35221 aattggaacc cctgaagcgg aatcgataag tatcttttga cttgaataaa gtgaccgcat
    35281 caacagattt tgctgctctt aaagaagaaa tattatccat tatccaaaat ccccggccca
    35341 ttgtactaag aattaaatca tcgcggtaaa tcttaatatc cgtgacagga gttaccggca
    35401 ggtttcgctg gaatttctcc caggtttcac catcgtcata agaaataaac attccgaact
    35461 ctgttccggc aaataacaat ccttctttca cctgatcttc acggactacg cgaacgggat
    35521 aattctccgg aattccattt tttccggtgg ttagtaatct ccaggtttta ccaaagtcgg
    35581 ttgtcctgta tgcataaggt ttccagtctc caagctgata ccgtaaaact gcgatatagg
    35641 ctttgccttc tttatgaggt gatggttcca cagcatccac gcgtccgccg gcggccaatc
    35701 ctttgggtgt aacttgttcc cacgtttccc ctccattcct ggtaacatgg accgggccat
    35761 cattggctcc tacccagata acccctgctt ttactgatga ttccctaatc gcgtatatgg
    35821 ttgaataaaa ttcctccccg gtgatgtccc tggtaatggg gctcccggaa ataacctgcg
    35881 tcttcggatc aaaagcagta aggtctggtg agatagtttc ccaggtaatg ccatcatctg
    35941 ttgttttgtg gacatactga gaggtgtgat aaacaacatc cggattgtgg ggtgacacat
    36001 gaatcggtga aactctctga aaccggtatt tcaaatcagc tggattatga ccatacatat
    36061 ttgcagcacc aacataatat tgtctttcct gaccggtacg tttatcgtaa acgccaaacc
    36121 ttcctttgca gttggaatat acgatattgt ggttgcctgg ttttggaacc gccggtccgg
    36181 tttcgcaacc accaactgca agccaatgtc ccgcatcacc tgcgggatga ttatatggtg
    36241 ggagagaagg aactgccatg gtagaattat cctgttgccc tgcatagagc caatacggta
    36301 cctgatcgtc tacctctacc tgatataatt ctgctgttgg ttgattttca atactggacc
    36361 aggttttccc atagttaatg gtaacattgg ctccgccatc attagattga acccacacaa
    36421 gagtatcttt cggattgatc cacatatcat gattgtctcc atggggtgtc cgcattctgc
    36481 gccaggtttt tccactatcc actgagtaat gaaatccggt ggagtttaca taaactgtat
    36541 taggattttg tggatttaca tccacgttgc aataataaaa aggcctgtcg gtaagtggcc
    36601 ggtaggatga tactaactca aacgactcac ctctatttga tgacttataa agacctcgga
    36661 catcgtcagg tgctgcttca accaaggcat acaaaatttc gggatcggca ggtgtaactg
    36721 ccaaatcgat ttttccaatc aaccctgttg gtaatccctg cgtttgtttt ttccaggttt
    36781 ttcctccatc aatagattta taaatacccc cttccttact tccactgata attgtccagg
    36841 gttttctctc tgcccgccac aaggttgcat aaataatatc cggattatcc ggtgcaaact
    36901 ccaaatctac tgcaccaata ctgtcagaat gatataaaac ctgactccag gttttgccac
    36961 catcagtagt tttgtaaaca ccacgttctt tattacgctg aaaagcttgc ccaatggctg
    37021 caacaaatac aatatcaggg ttggaaggat gtatttctac ggcaccaatt tgtcctgtca
    37081 ttggcaaccc ggcaaaagac caggattttc ccgcatccgt tgacttaaaa acaccttttc
    37141 ctgtaattac attacttctg attccatctg aacctgttcc tacataaata atgtccgggt
    37201 ttgagttcgc cacacgaatt gctccaatcg aaggagtgga gaaatacccg tcagaaatat
    37261 tctgccagga atcgccgtaa tcttcagtct tccatacacc accaccggta gctcccatca
    37321 ggaagaaacc cggcttttcg gaatgcccgg ccacagccgt tgcgcggcct cctcttgttg
    37381 ggccaagatt ccgatattca taagcctggt aaagtttctc gttatcagaa tttacattgg
    37441 attgactttg tccttcaaat ggaaccgcaa ttaatgcaag gacagttaga atggatgttt
    37501 tggcaaattt catggataag tgatttagtc tctgaattta gaaaaaaaag gtcacttaaa
    37561 aagaaagaat aggttgaatg cagctattaa tttgcgagtg gtaatgtgga ttatggcata
    37621 tacttccagt tccggggttt cccttcctga agccattcca tcattgtgct aattcgcttc
    37681 tcccgggtcg tctcagtttt tgcttcctca atccattcga tgtaatcttt tttgttagaa
    37741 tttgaaaaat tttcaaaaac tgaaaatgct ttcttgttag ctcgaattat tttcaaaatt
    37801 tctgaaggca cccttacttc ctttagtcgc tttaccgttt tcttcgggtt ctttatgcct
    37861 tcatctttaa gcctgactgc ctgatggatg taatccagaa taatcttgtc acttggcaga
    37921 tctttaaggc ttgtaatttt gccaaaatgt cccatcgctt cccctgatgt caggacccca
    37981 tgattatctt taattaatgc tccttgccag aagccaaatg tgcagtgcgc cttgaaggcg
    38041 gccatgttgc aaaaaattcc cttgtattca aaattaggaa aactccattt aattttttcc
    38101 gcgacttcag gacatccctt gtggatgagg ttacgaatgt gtttcaaaat aggtttggca
    38161 aagtcagctg acctggcaat ataagcgtca atttttggat ctttctttcc catgattttg
    38221 atttgagatt tcaaaattaa caaaataatt caaacgaatt aaagtctaaa ttgaactaat
    38281 attttgtcca gtttttgctt tggatttttt cctgttgatc gattattttt tcgatccaaa
    38341 aatcgtcatt catctggttc gcgatactta aggctctttc actggccgaa agcgcctgaa
    38401 catattttcc ctcccggtat agaaaccaac tatactttgc ccaggtcata taatcagatt
    38461 caatctcgta attatatttt ataccggtaa aataatactg ctggtcattt ggcattttgt
    38521 agataacaat gcacaaaatg agtattacca ggttaatcca ctgacgacca tctgccaaag
    38581 ttgatttgtt cctgaactta aagtaaacac caaccaccag agaaatgaat gaaaagctca
    38641 tgggaattat ccccatggga aagaatttgg taagaaataa aagccagatc agcaaaaagg
    38701 ttgatgaaag accaatgaag aaggaatcca tttcaatttt gcgggttgta accatgtata
    38761 tatagagtcc caataaataa ataatggctg caactataaa gtaggtgtga aagtgaatcc
    38821 tgtagatttt caatgtgtac gcgatgaagg caaaaatgaa accggcaatt tcttcttttt
    38881 tcattgggat ttgcttgcaa cgaatcaaaa gcaaaaaaag tctttttcca aaagaatttg
    38941 ggttaaataa agtgttatat aatcatacac tatactgtat caataatgat acagtataaa
    39001 actaattctg gcaaataatt tacttcttag ccgctgataa tgaatacttt aacaccatgg
    39061 cactgttttg gtttccataa taacatcaca cttaaaaaag accaagaatg attcatcatt
    39121 atctgaaaat cgcttttcga aatcttgttc gacaaagatt ctatactctg attaacattt
    39181 ttggtttggc tctcggcctt gcctgcacgg ttcttatcgg gcactatatc cattatgaac
    39241 tcagttatga cacgatccac cctgatgtgg agaggcttta cagggtgaat caaactaata
    39301 tctggtatcc ggaacaaggt gccatgagtt caaccggact tcctctggct aaaattcttg
    39361 cagatgactt tcctgaagtt gaatcctcat tacgtataaa cactccctat aggtttacag
    39421 tcaggtatga tgattctttt ggaaatataa aagcctttaa tgaagatgat tttctggcat
    39481 ctgactcaaa tttcttcgag tttttcgaat tcaaattaaa agaaggtgat ccggcaactg
    39541 cattaaaggg tttaaacaaa gtagtaattt ccgatgaagc ttctgcaagg ctgttcggag
    39601 aagaacctgg cttaggaaaa attatcctga tgggtgaaga tcgaattcct ttggaagtta
    39661 caggtgtaac cgaatcccag cctgaaaata ttcatttcga ttttgactac ctgatttcta
    39721 tttatacgaa tccaaatatt aaacgatttg agtggagttg gatttggacg caggctgtta
    39781 cctatatcaa attaaaggag gacgctaatc cggaggattt ggagggaaag atggagagtt
    39841 tggttgacct tcatgttgca ccttcgttta agagattcgg tatagatttc agtgaattta
    39901 ttaaaggagg cagctggaat ttttatttgc agcctgtcac cagaattcat ttatattcag
    39961 cagttgaagg taacaggatt ggaaacaccg gcgacataaa atatatctat attttcggcc
    40021 ttgtgggaat ctttatttta atgattgcag ttattaattt tattaatctg gctaccgcga
    40081 gagctgggat acgtgccaag gaaatcggaa ttaaaaaagt tatgggctct caaaagagaa
    40141 atttgatcat gcagtttctc tccgaatcca tgttagttac ttttataagc ctgggagtgg
    40201 gtctggcggt gatggagatt ataaggattc tgctgaataa atatctgcta attccgatgc
    40261 tgttcagtat ctgggagaat ccggtactcc tgctaataat gccactcttc gcgattatta
    40321 ttggaattct ggcaggactt tatccggcgt tctatattac cagacttgcc cctggtgaaa
    40381 ttttaaaggg cagaattacg cgtggcgtag caaaatccaa cctgcgtaat ttattagtgg
    40441 ttgttcaatt taccatttcc atcatcctga ttgccgctac ccttattgtt tcacagcagg
    40501 taaatttctt tctaaatagc gatctaggtt ttgaaaagga aaacattttg ttaattaata
    40561 actccgagag acttggtgga caactccaat cctatgttga tcaattggat tcagaaacag
    40621 caattaaagg cgccgcaata tcaatgctca ggccagggcg tggtggtttt gaggatatat
    40681 attctgtaga aggatatgag gaaaagtatc caatgtcgac agaaaaagtc gatgcgaatt
    40741 atttttcaat tatgaacctg aaatttcggg caggaagacc ttttgctgag aatagtatct
    40801 cagatcgaaa ttcggtaatc ataaatgaaa gcacggcaag attatttaac tggacaccgg
    40861 aggaggcatt atcacatcga ttgaatgatc ttggagatga gttggaagca gtgcccataa
    40921 taggtgtagt taaagatttt cattttcagc cccttcaaaa taaaattggt ccactattga
    40981 tttttcatga aaatggagca atgtggggag atatgcgggt ggttattgta aaatatgata
    41041 caaaaatgga aagtcaattg gtaaatctgc ttcaatcaaa atggcatcaa attctgcctg
    41101 atactccatt tgaatatgaa ttcctggacc aaacgctcgc tgaacaatat gattttgaaa
    41161 aacaactggg tggattattt agttttttca caacgctatc cattgtgatt gcaattatag
    41221 ggttaattgg tttggtcgct tttgcatccg agcaacgccg gagagaaatt gggatccgga
    41281 aagtactggg agctccagtt cttcgaatcc tggtgatgct gaactcgcac cacctcaaac
    41341 tgattgtttt aagcttaatt tttgccgtac cggtcgcctg gtatgcaatg gacaattggc
    41401 tttcagggtt tccatataaa attgaaattt ccccctgggt atttttactc gcaggcggct
    41461 ctgttgcctt cttgtctctc ctctgtgtca gttatctctc catcagagct gcttccctta
    41521 acccggcgga agtactgaag gaagagtaac caccggtgca attggaattg taagagaaac
    41581 tcaatttact atattgaacg caatccatcc tatttagcat tggtcagatt tatgcatttt
    41641 cattatggct gcagtccagg ccatatcaag agcctgtctc tcttccggta agcttgttcg
    41701 caataagttc tttggcactt ttgaaagcgt tttgtgtcgg attatcgccc gcgatcattt
    41761 tagcgatttc cgcaactcgt tcttcttcag acagctcacg gattttgctg accgcttttc
    41821 catccaggtt ttccttgtac acaaagaaat ggttgcttcc cctggcagca atttgaggta
    41881 agtgactgat ggtaattacc tgatgtcccc gggccattgc cttcatcatg tttccaagtt
    41941 tcagggctgt ttcacctgaa ataccattat ctatctcatc aaaaactatg gttggcaatc
    42001 gggttttttg agcaagaatg aatttgaggc aaaacattaa cctgctgaat tcaccaccgg
    42061 aagcaacctg acgaagttct tttggtttta tgcccttgtt ggcagaaaat aaatatgaaa
    42121 ccttatccat tccttgattt cccgcctcaa cgtcagttat ttcaataaca atggctgcat
    42181 caggcattcc cagatctttc agatatccgg tcaattcttt ttgcaattca ggaacaactt
    42241 tccttctttt ttccgagagt aacctgccgt atttctctat ttcatcccaa aattttcgga
    42301 ctttggcttc agcttcttta atagatgact ccatccctga tgcagtattc actttttgtt
    42361 ccagcacttc caggatttcc accaattcct ttatagtact taccttgtgt ttttgttgga
    42421 gaccgtaaat caggctcagc ctggatttta actcttcagc acgcgccgga tcggtttcca
    42481 cattttcttc ttccgttgca aggtctctga taagttcttc aagctcaaaa gagagatttt
    42541 ccaatcttga tgaaaacaac tcatatcggc tggaaaattt agtaagatgt ttcagcaacc
    42601 gtaaactttc ttttacctgg ttcagaacag aaatttcaga atcctgcatg gaattcaaaa
    42661 ttagattcaa gtttgattta atctcttctg aattttctaa tacctgaacc tgttcttcca
    42721 gattctcctg ttcatcttca acaaggttag tcttatcaag ttcttccttt tgaaaattta
    42781 tataatcaaa ctccgcggat agtcgggagt gttcttctct aagttgtttc agattttttt
    42841 ctgaatgtct gtattttgca aatgcagatt tgaagtttgc aagaatttct gaggttccgg
    42901 cataggaatc caaaacttca agctgaaaat ctgatgatcg taataaaagt gtgtcatgct
    42961 gtgaatggat atccattaga ttgttcccca attctttcag aatgtctaaa gtaaccggag
    43021 tatcattgat gaacgcgcga ctttttcctg aaggtgaaat ttctctccgg ataatgcaga
    43081 ttttatcata atccaaatca taatctttga aaaaaccggc caaatcatat tctgaaatgt
    43141 tgaagtctcc ctcaacataa catttatcac ctgcatcata caaggttttt gtatccgctc
    43201 gttctccgag caataagccc acagctccca acattattga tttaccggca ccggtctcac
    43261 cggtgataat cgtaagagcc tctgatggag agaatctaag ctctttgatt aaggcgaaat
    43321 tggaaattcg taattctttg agcatagcac tttggcaata ttaccaattt taccggagga
    43381 ataggaaacc ctgcagaaag gagactgaaa attaattttg cagaatttta tcaaagcgat
    43441 ttttctttgc aggatcaatg ctgatcagca gactgtgagc ttgtctcttc acattcggag
    43501 atgcatcgcc gtaaatgttt accaattcat cggtctttgc atcaacaaat gcggtgatca
    43561 atatttgact tggattcctg tcatttaact gtttcagatg agtaagtact tccagaatct
    43621 tctgctgggc gtcaggcgtg ttttcaaggt aagtatccag ggctaatctg tgataagtat
    43681 agattccctg ccttatttct tccagtgcct gtcctgtaag attttgggcc agccagtacc
    43741 tgtttctatt acttcgctgg gggctccagc caaggaagtt actgggctga gcttctgtaa
    43801 ccacattcat cgctttattg aaaaagggtg taccgcctaa ttcactaaaa gaatcataat
    43861 caaatcccaa tacaatgaaa gcgtaatagg caaggagtga agtgagatta ttttcaaaac
    43921 gggtttccac gaagtccatt ggctgacttt ctgtgtaatc aaattgccac tcacggtcag
    43981 caaagtttag caagatggat tcatagttgg tattataaat tggtcttgct gattgaatct
    44041 gaactgtagc ttcgtaggtt ccaatactaa ccatcttgtc aatggtaaca ttgatgttgc
    44101 attttattct ttcaacctgt ctatatgtat ccgttgtcca tttgcgggag ttaagaaatt
    44161 gctcaaatga agtttccata tcagtaaaaa tacgcttatc agacagggca atctggtctg
    44221 cattaacacg cacactgcaa ttaagttcct gggctgagga tgaatggaac aaaatggttg
    44281 ttgcaaaaag gctagccgat atattccaat atctcattga taatgtcatt tgccacatcc
    44341 tttttactct ttaactcaaa atactttttt ttattatctt tcctgaagat ggaaattttg
    44401 ttggtgtcat gtccaaaacc tgctccttta tcttgcagcg aattaagcac aattaaatca
    44461 aaattctttt cctttagttt ttttgccgca ttgcgggtct cgttttcagt ttcaagggca
    44521 aaccctacca gcacctgatt cttctttttt acttttccta attcttttgc aatatcaggc
    44581 gtacttgcta attccataga taatacgccg gtcttcttct taattttatg cctggtaaaa
    44641 ttgcttgggg aatagtcagc aaccgcagct gccaacactg caatatcgca tgatttgaaa
    44701 tgtttttgac aagccttaag catttccttt gccgaggtga cccgggttat atgaatatta
    44761 tcagacttga catctaaata ggttggcccg gagacaagtt ctactttagc tcccatttcc
    44821 gcaaatcttt ccgcgattgc atatcccatt tttccgctgg aatggtttcc aataaaacga
    44881 accgggtcga taggttcata agttggaccc gcattaatga gaaccttttt tcccttaaat
    44941 ctttttttga ttaaaaaaaa accctgaatt tctttcagaa tatgttctgg ctcagacatt
    45001 cttcccaggc ctttaagtcc gcttgccagt tcgccatcct ctgcctcaat taaaatgttt
    45061 ccataggaac ctatcttcat aagattattc tttgtgcttg gatgggcgta catatccaaa
    45121 tccatcgccg gagcgatcat tacaggacat ttagcggaaa gataaattgc aagcagcaaa
    45181 ttgtcacatt gcccattggc catttttgca agtgtattgg cggaagccgg agcaataatc
    45241 ataaggtctg cccataagcc taattcaaca tgattattcc attcaccagc atcatttcta
    45301 ataaaatccg ttagggccgg gtgctttgaa agtgtggcca gagtaagtgg ggtaatgaaa
    45361 tcgtgggcag aagatgtcat gattacctgc acgtctgcac cctccttaac caggagtcgg
    45421 gtgagcagtg cagatttgta agcagctata ctacccgtaa caccgagcaa tatttttttg
    45481 cccttaagca aaacttagag ttttatacca ccctcttctt cctcagcttg cctgtacaag
    45541 accttgccgt cagtgaattc ttctgtggca attattgtcg gcttgggtaa tcgctcatag
    45601 aatttagaga tttcaatttg ttcccggttt tcaaaaatct cttcaagatt atctactgtt
    45661 gaagcgaatt cagcaagctt actattgagc tcttctttaa gattggtcgc aatttgtctt
    45721 gatcttttag aaatgattgc tactgtctca taaatatttc caacctcttt ggacaagcca
    45781 gtaagatccc gggtaataat agatggattt gccatatttt tgataattaa ttttctgcta
    45841 ttttgttaag tttttcagaa ctatcctcat ggatactttc ggcgtctttc cgaaatgatg
    45901 tattagggaa tttctcaata aattcctgat aaaggcgtac ggtctccctg tacctctcct
    45961 ctcttttatc aggtatactt agttttgcca gattatattg tgcatctagt ttcaggaaat
    46021 agatttcttc cgcaaaaaca gaggcgggat agtcttttgt aaagttgtca agtgctatta
    46081 ctgctgactg gaatcttcca accttataat attgtttaga gttctcgtac gctttctttt
    46141 ccagcttcac ttgcaattgg tctatgattt caatggcttc aggcctgtat ctgcttgcgg
    46201 gattcttgtt aaggaaaatt tgcatagcaa caatggcttc aaccgtgctg gattgatcca
    46261 gattataatt tggagattca cgatagaggc tataggcata catgaactca gcttcctggg
    46321 ctaatgggct gcggctataa gtctggtaaa aggtcctaaa attatgtgat gccagtaaat
    46381 attgaccctg atgataatag gagtaagcgt aataaaatag aatagtttcg ctttccggca
    46441 ggcctcttgc aattggcaaa acctcttcca acaacagatt agctttaaaa taatcttcct
    46501 tttcatagta cttcatggca gcttcgtact tcaccctcca gtcaggattc ttctgaatct
    46561 ttctgaattt tccgcatgat gaagtgagta tgacaagaag gactaaaact atccaaccct
    46621 gctggtttcg cataagggtg caaatatacg gttatggact aaaattacca aaaaccttgc
    46681 cgttccagtg agttgaaaat gggtaaaatg gctttacttt tttactacaa atttcttggt
    46741 ggtcacgctt ctggtattaa ggtagagcgt atagaaatat acccccggat taaggtcttg
    46801 tatgggaatt ttaagctgac tttcgtgaaa agaaagcggg tgtaccccaa caatgctacc
    46861 aaggacatta tggataataa ttttgacttc ctgttcttct acttttaatt gataatccag
    46921 aaatgcattc tctgaggcgg gatttgggta gatatccctt aagaatattt cagctgactc
    46981 ataaaataca tctccacggg ctgcctcttt aactgagtaa tgcacatcaa aggaaatagc
    47041 atcatcagga ttatccttgt tatatatatg ataggaaaca atgctgaact cctctgtcag
    47101 acctgcatcc agaatactta cgaaattgct ggttatttca ccaggttcca acttcttact
    47161 tataatcaat ttttgtgcat ccggttcaag acaatccccg ctccaacaaa ggcgagttag
    47221 ctgtgtggta cctatttcag tctttactct ctggattacg atagaaaccg gagtagctga
    47281 tatattctgg attctgatgg tggcttgtat tttatcaccg attttgccct cccaattctg
    47341 ttcgttgtca agcaacttta aactttgact aatggcagga ctaaaagcca aaattaccaa
    47401 aattgctatt cctgattttt tcacatcaaa accctttatt cacacacatt ttgacacaaa
    47461 attacatagt ttctacccac taattcaact aagaaattaa gaaaatgtta catttcaagc
    47521 aaggaataac aatattatag ttatgcaatc gttgcaatta gctctgtttg ggggatagaa
    47581 tcaggtcaat taaccagctt ctgcaataat ttcagctccc ctaatacttc catcagggtt
    47641 ggcttttctt ttattctcca ggtgaagcct ttgagattcc ggtcggctgg ttttagctca
    47701 tggggcggga taaatgatgc ctcaacgctg gtgtaaaaat gaacttcctt tgctttgttg
    47761 ttctcaaaat tgatggtgat attactgctt gtgctcttat ttaagccgat taagtctgtc
    47821 tcttcgtcat tcaaggcata aaaaagactt tctccgttgc cggttacctg aaccagactt
    47881 attttattgt ctttgaaaaa ggccaggaca ttccttcctt tcacctgatt aaaattggat
    47941 agtgtgtcct gcgaaactat aaaagaatta gaaacaagct tcattttgtc aatcttctta
    48001 ttcactatga aggcactaat tgtgtccgca attatctgat ttccttccgt ccataaaatt
    48061 ggatttgtaa agaaatggat taaggaatca gacacttcgt aaatcaaaga atcggcacga
    48121 ccctgtaaat cttctttaaa aaacttaaca ttatggtacg caatcaattt ttttttcgct
    48181 tcaatatcac tgtcataggc aacaagcgta tccgccgaaa gaaataatgt atctccatcc
    48241 atcagaacct tcaaaacggg ctggtcgaat acttttgtaa ttcccgttcg tttagaatat
    48301 atagctgatt gcccggtaac aataatatca ttttcagtat ctaccagttc cacattcctc
    48361 ttggctcgat attcttgctt tttatcatca aggttgaaaa tatttgcctt gataatataa
    48421 gattctgttc gaactatatt tcgttccagc aagcttgttt catcaagagt gttataaatt
    48481 ccttcatcat actccagcac attaccctcg tcatcaatga gtattgagcg atcacgaaag
    48541 taaattattt tagttgctga attgtactgc agcgtatccg aggttagcgt ataattctta
    48601 ttctttccga ctacgttgtg tttaaatgag gccatgtttg tgtttacatc ataatacccc
    48661 ctacggctgg taagtgtatt tgtactatct actaattttc ctccggaaaa ataattggcg
    48721 acctgactta cacggtcata atccaaatta tcagtataaa gagtcataat gccgagcttt
    48781 gtgaagatta cattatttct aagttttgcc acacgggtat ttccatcata aatgagtatt
    48841 tcactggtta tgtccacact gtctccgtca ataatatgca cattgccttt agcttcaatg
    48901 taacttcttt tcttgaaata gatggcttca tcacaataaa tccaggtatc ttctctttta
    48961 aatattactt ctcctattaa tttttgatag tcttcaccat tcaactttcc cccaataaat
    49021 tcccttgaat tctcaattct aattctgtcc tgcgcaatgg aattcatagc aatgccccaa
    49081 aggcttacaa ataatattga tatttttgca tgtatagtca tagcaaaaag gcaaggcctg
    49141 tttaaacaag ctaatgttgc aaaagtagca aataaaatgc cccataactc agaatcttca
    49201 aatactcatg aatttgaaag agctttccag agattcatca tcaaggaaaa gctggttcca
    49261 acgcgttcaa aaatattact tgcagttagc ggtggtgttg attcgatggt tttgctgcat
    49321 ctcttccatt cgttaaagta caggattggt gtggcccatt gcaactttgg tttacggggt
    49381 aaggaatctg attatgatca aaaatttctc aaggaataca ccaggaaaaa caatattgaa
    49441 ttctacaaca aaaggtttca aaccaaagaa tacgctaaga agggaaaatt aagcgttcaa
    49501 atggcggcca gggaaatccg ctatttctgg tttgaggaaa tcaggagaaa acacagttat
    49561 gatcttattg ccaccgccca tcacaaagac gatgctattg aaactattct cttaaatatg
    49621 tttcggggga ccggaattat ggggcttgct ggtattctcc cccaaagggg gtgtattatc
    49681 cgtcctttgt tattcgcgaa caaaaatgcg gttatcgaat atgccaggag tatgaacctc
    49741 gcctggagag aagacagttc aaacctggat aacaaataca gtcgcaatct tttgcgaaac
    49801 aggataattc caaacttaat aaaggaaaat ccaggcctga gaaaagtact ggacaactca
    49861 ctgagtcgat tcaggattgc cgcgcagctg ttgcgtgatt acagcgcatt aattaaaatg
    49921 aagtttgtcg aaatgcaggg tgaggtgttt agcatcaaca tgaagattag aaacattgct
    49981 tattctgaac ctgttctgta cgcgataatc aaagattacg gattcaatta tacccaggtg
    50041 aaagaaatgc tccacgcggg cagttctccg ggaaagaaat tttgttctga tcgttattgc
    50101 atctttgcag acagggagaa atggatttta atcgaaaagg aaattgaagt tcaggaaaaa
    50161 ttactaatca ggaaaagcac aaaaagaaag aaaagcaggt tgggggattt caatatttca
    50221 agggaaagag gtttaaagaa aattattggt gattttaata ctgcacacct tgatttcgac
    50281 aagcttagat tcccgcttga aattcgtgcc tggaaagaag gtgataaatt tcaaccgttg
    50341 ggaatgagag gtaaaaaaaa gattagtgat cttatgattg accagaaaat tccagtaaac
    50401 ttgaaaagga gagttaaagt aataacctcg ggtgatgata ttgtttgggt agtagggatc
    50461 agaattgatg agcggtataa gattacacct caaacccggg aattttacat aatgaaattt
    50521 gtcagcaatg attaatccat ttaagaagac ttttacgccg caagagatga gcctattcag
    50581 gtttttatcc aaggttaaag tttttgaaga tttggattat aaagagttat catattttgt
    50641 tccctttctc cacgaaagag aatataagag agatgaaata gttttcttcc ggggagatcc
    50701 aagccaggca ttctatatca tcaagaaagg cagagtttcc ctgtccgttg acattgaaga
    50761 taagtttgaa atgcttgatg agttgaaaac taaccagtca tttggcgata atgcctttct
    50821 caataatacc cgaaggatat acacttctat gataacctca gatgaagcaa ttctttatgt
    50881 aattcccagg gtaaatattc acgaaatttt tgacagggaa gtggaggtaa aagccaaggt
    50941 aatgcaatct ctttcctccc aatacaatga atatactacc aacttgttca aggcctacca
    51001 gtcctctttt ggtttttttg acctgggcaa ggtatataaa agttaacaca ctcgtattta
    51061 tcctttttct tgctgtcatc tttatataaa ttagcgtcct ttccaccaaa aaataaaaag
    51121 aaattattat gaaaataacc gttgtaggcg caggaaatgt tggagcaacc tgtgctgatg
    51181 tacttgctta cagagaaata gctaatgaaa ttgttctgtt ggatattaag gaaggacttg
    51241 ctgaaggcaa agccctggat atttggcaga aggccccgat aaatcagtat gacagctaca
    51301 caacaggagt tacgagtgat tattcgaagt ccgcaggctc tgatgtgatt gtaattactt
    51361 caggattgcc cagaaagccg gggatgagca gagatgactt gatagaaacg aatgccggaa
    51421 ttgtcaaaat agttaccgaa agcgtagttg ccaactctcc caattccatc atcattgtcg
    51481 tttccaatcc attggatgta atgacttacc aggctcatat tacctcgaag ttcccgcgta
    51541 caaggctaat gggtatggca ggtatcctgg atacagcccg atacagagca tttctggctg
    51601 aagccctgca aattagccca aaagatattc aggcagtatt aatgggagga catggggata
    51661 cgatggtgcc tctgcccaga tatactacag ttgcggggat cccggttact gagttgattg
    51721 attcagagaa actaaatgct attattgaac gtacaaaaac aggagggggt gaattggtaa
    51781 aactgatggg tacttcggcc tggtacgctc ctggtagcgc ggcggcgcaa atggctgaag
    51841 ccatagttaa agaccaaaaa agagtttttc cggtatgtat taagcttgaa ggcgagtatg
    51901 gtatagataa ttgttatctg ggagtaccgg ttatacttgg gaaaaatggg attgaaaaaa
    51961 taattgaatt gcaattgaat gatgaagaac aggcaatgtt aaatacttca agagaccatg
    52021 tgaccgaagt aatgcaagtg ctggatcgat tgaattctaa atgagcaact aaccttttaa
    52081 ccaatgagga aattatataa agttttttta ctacttattt ttttaagcgt ttttgagctg
    52141 gcaaatggcc aggatgtaaa attaccggga cttgatccat ctcccgcaga cattgctatt
    52201 ttgagagaaa gccgaaatgc ggacccccat gcaaaaatta tatacagcag accacaaaag
    52261 aagggtcgcg taactattgc agctgataat ggagcattag ctccttttgg caagctttgg
    52321 cgcacagggg ccaatgaaac tactgaactg atcgtttaca aagatatcaa gctcggaggt
    52381 gttgacatcg ctgccggcac ttattcattg tacactatcc ccggagaaaa agagtggacc
    52441 ttaattctca acgggaaact caatacctgg ggacatttcg cttacgatga gtctcaggat
    52501 aaatacaaaa ttaccggggc tgtgaaatct tatgaacctg ctgttgaggc attcactata
    52561 gttttcggag gggtatccgg tggtaaatct acgctatacc tggcatggga tacaactatt
    52621 gttgaaattc ccgtggaatt atagaccttg gaattgtatc agcaatgaaa atgattgagc
    52681 cccgtaaggg gcttttttat tatgcacacc tggataattt ccggttttgc gcctgaaatt
    52741 tgattatcaa cctgaaattt gacaatcaac ttcagatgat agaaacattg attgcttctt
    52801 tccttttaca caagaaattt attcactctt tttttcaagt cttccctgtg ggaagtaatg
    52861 gaaggatcgc tgtaaatccg gctgcttagt tctgaaattg cttccttctt gtatccaagt
    52921 ttagcggcta catcctcagc aaatgaaatc tcgcttttga aaacctggcc atcgaccttc
    52981 atcatttgaa caatattgta caggtattca aatttttgat catcagtcaa tccggataaa
    53041 cttcccatcg gctcaggagc ttccatcatg cctttgatca cttctttaga taatccattg
    53101 gccagtccga ccgtcattat taacctggat tccttttcgg caatcatacc atcgctaacc
    53161 gcaagctgaa tcattatctt aagttgttcc ttaatcatga cattgacaat tggtggtgaa
    53221 agccaattta gcaaactatg tggtaatatc gtgcgggttt aaaaacaaat ggggcctaaa
    53281 aatgccccat tttgctaact caaccttagc ttaagctcaa agtgaggaga aatgctcgat
    53341 tttcgagtct aaattggcag aatcagtcct atttacctga ttttctctgg cgacagcctg
    53401 gaaaagaata actacggcga ttattattcc aaaaatgagt gccagatttt ttctaattgc
    53461 cattttaatt tctttgtaaa gggataccgg caattttacc aatggttaca gcttcaaaat
    53521 aaatatattt gcgcgatgca actccgggaa ggcttcccag tctttgttat agaattaatt
    53581 atagaattat gaaattaatc ataagcagaa ctttaattgg tatcttcttg ctggcttcat
    53641 tttcggttct atctcaagac agggaggagc gaagtttaga ctcctttagt aaaatgagag
    53701 tcggtgaagg cattaaagtt gaattgaaaa aagggacttc tgaaaaggcg attatagaag
    53761 ttagaggtct tgacgtggat gatgtggtta ccgagattag aggtgggagt ctgaatatct
    53821 atttagatgg gaattggcac agaaatattg atgtttccat cgaacttact tacaaatctt
    53881 tgaactcaat aacaatcagc agtgctggaa atgttagaac gaatgaagct atagaatccg
    53941 atgatttgta tataagcgcg tcaagtgcag gaggaggcga catcgaggta aaagtaaaag
    54001 aactggagtt ggaagcttcc agtgctggaa ctttagatgt ttacggttcc actattgagt
    54061 tggaagccgc agcttcgagt gccgggagca ttgatgctta cgatcttaaa gccgaagaag
    54121 tagtggtaag agcttccagt gccggatcca taaaagttca ggcaaccaaa ctcattgatg
    54181 ccagggcatc cagtggcggc agcataagat atcgtggtaa tccctccaaa gaatacacaa
    54241 attcttccag tggtggatcg gtaagaaaat cggggtaggt aaaattacta taccttataa
    54301 tgttgtttta agaaccgtct gaattccttc tctcaaaatc tctatttcaa tttcttctcc
    54361 ttgcttgaac ttactcaggg cattcatgta agcataaata tcatcaatgg aaagttcgcc
    54421 catcctggta attatatctc ccttttgtaa tcccgcaaca gccccgggct tgccttccgt
    54481 tactccatct attttaagtc cttttccttc ataaacataa tcaggaataa tacccaaggt
    54541 aacggtaaag cttggcgctg aacgtgcatt ggaatctaca gtttttgaaa aggcaatagt
    54601 ttcaaaatcg tttaaatcag aaacaatatt gaaaataagc tggtaaacct gcatcatacc
    54661 ttcatagttt aaggtttcag aatcatcacc aggcttgtga tagtctgaat gagttcctgt
    54721 gaagaaacca agtgcaggga tatctttcaa ataaaaggag gtataatctg aaggacccac
    54781 accgctagct gatttaacta aatcaaaggg tgtggtttgc agatttaata aagagtccca
    54841 ttgcggtgag gttcccgaac ccattattga aatggcattt tttgtagtat ccacgcggcc
    54901 caccatatcc atattcagca tatacttaat ctttgaaaga tcaatagtag ggttatccac
    54961 gaaatatttt gaaccaagga ggcctctttc ttcccctgaa aaaagaataa aaacatagtt
    55021 ttgattgatg agtccggaac ggcctacgcc ttttgccaat tcaataacca ttgccgtgcc
    55081 gctggcattg tcatccgcac cattgtgaat cattttatca tctccgcgat aaagtgatcc
    55141 gcctccctcg ccataaccca gatgatcata atgtgcaccc agcaccgcga atgaatttgc
    55201 accgttatcg atccatccaa ttacattatg acctaccctt cgctcttcca ctaattccac
    55261 agtaataatt acatttttac ccgcaagagt ttcggaagtc tcatttttaa taaaagtcac
    55321 agggatttca agcgaagtaa tcttttgagt ataatgcttc tcaggatccg gggctgtcgc
    55381 gtcaatatta taaaaaatta ctcctgacgc ccctttctct tcagcgactc ttgccctttc
    55441 tttaagatta tgataatcca gatactttga atgggggtga atcccgtcag gtgaagatat
    55501 attgataagc aggattttgt ttttaagttc agttagcttg gcgtagtcat cataacccag
    55561 ttcaagggca tgaattccaa atccaccgtc aataattttt ccttccgcca tagtattgcc
    55621 agagaaattc agagggtaaa agttttcatt atttacttga ttatcatcaa tggaaagggt
    55681 agtgcgttcg cccacttctt ttgatgcaag aaaatcgaaa gcctgaagaa atgtattgtc
    55741 atctccgagc ggcttcaaac caattttctg aaattgctca acaatatatg ctgctgcttt
    55801 ttgctcaccc gtggttcctg cttcccttcc ttccaaatca tcggaggcca aaaaggcaac
    55861 atgaccggtg aactcaggtt cagttttcgt ttgacaggaa tttaccaaca gcagtgtgcc
    55921 gataatcatt agaattctat tcatggggaa ttttatttgc aggttgctga atacttgata
    55981 atgattatgg attcatcatc cccggatttt tcaattgctt cttttatcac cgtttcagcg
    56041 aatgatttgt cttcgatatt tttcagcgga gcctcaaggt taaaagccaa atcataattt
    56101 ttcttaatcc attttacggt ttgtttttga aaccttcggt atttgggaga cttgacgcaa
    56161 catgttattc tgatgggttc ttcgctaacg atcctgatgg tgttatcttt ttgttttatt
    56221 tcggtgtcta tccacacata ccagatttct tcctcctgcg cgtaagcaaa tgaaatcggg
    56281 aataagaata aaactgcaag caagtatttc atgaagcaaa aataattcaa taccgcatca
    56341 gaaaaaaagt tcagctacga gcttttcgaa ttaaaaagaa taatactgca attaccgcta
    56401 atattaatac tatttcccag aatcccatta ttcattcatt gattgttcgc gtattgcttt
    56461 aaattccgat cttttccctt cattttggaa atttgaacta ttgctaagat cgattcttat
    56521 ttgataaaat aagctggcat cctcaaccat tcacctccaa agtaaatatt ttgtatcatc
    56581 ttatattttg aaagtacttt tttttgataa tcgaagttat taatgccaaa atcaattcgg
    56641 tgatcgttca tttaccgata tctatatgta cgatttcgga cacacttata tctcaagcct
    56701 ccaaaatttg cagttttttg ataatttatt gtttggtata atgcttgaaa cttaaattga
    56761 aaaaaaataa tgatatggca tcacaaaagc tagttcgctc caaggataaa atcattgcgg
    56821 gggtttgcgg aggtatcgct gaatatatgg gggtggatcc aaccgtgata agaatcttat
    56881 atgttttcgc ttccatcttt accgcagcat ttcccgggat tttggtttat atcatcatgt
    56941 ggctcataat gcctgagaac taaccgtggg cctctgttca gcaagtctga taatgaaagt
    57001 agatccttca ccaggtttgc tttttaaatc cactgaacct ccaagagatt cgacctgact
    57061 ttttatcagg aacaggccca tgccttttcc ttcaacatga ttatgaaatc tattatacag
    57121 attaaatacc ttgtctttat gaatactaag gtcgataccc aatccattat cctggacagt
    57181 taacaggata ttatcgtttt ctttcctggt tgcaagaatg atttccggtt gtttttctgc
    57241 agatctgtat tttattgcat tacctatcag gttcaacaaa atgctataaa ggaaagattt
    57301 agaatacctg atttcttgaa tttttaaatc caacgtaatc gatgcattgg attcctggac
    57361 catcggtcca agacttacca gaacactgtc aatgacagat ttgaagttca gaacttcggc
    57421 agtttcctga gaacttccat ctgagacaga aataatcttt gtcagatcat ccagggtatc
    57481 gtttaaggtg ctggccagct gattgatttt cgaaagcatt tctttgttat cggcgctaat
    57541 ccgggactga tcgaccaatg agagcaagga gctgatatta tttaccggag atttaaggtt
    57601 atgacttgtg atataagcaa actgtttcag gccataattg gattgctgaa ggctttttgt
    57661 caattcaacc aattttcttt ctgcctcctt tctgtaatga atatcaagat gtgtaccggt
    57721 cattcttatg ggtgttccat ctgatttctt tttggttatt ttacctcgat ccaaaaccca
    57781 acgccaggtt ccgtctttcg tttttatccg gtactcggaa gcatagaatg gagttttgcc
    57841 tgagaggtga ttatttagcg cagttattct actttttttg tcttcgggat gaatcatcat
    57901 ttcccttttg gataagttcg ctgcaacctc agatacttca taacccagca tggaggccca
    57961 tttagtatta attataaccc tgtcagtttc aatattccaa tcccaaaggc ccaaatcggc
    58021 tcctgtcaaa gcaaactcga gccgttcttc actcttaaca agtcttttct gcgggtcatt
    58081 ttcttccttg ttcccggaat catccagaac cacgcccaat atttcggggc tcaccttata
    58141 gtcaacagaa cgctcattta tggtcccaag aagtaacatc tcattttccg attgtaataa
    58201 actgatcgaa aggaaaaccg gataaggttg tccgtctttc aacttaatta aaatcttttc
    58261 atccttaatg atcttgcctt tcttcagttc tgccattatt cgaagcctgt cattcggatc
    58321 ataatataaa ttccgtatat cgcccccatt aagtaattcg tcacgtgaat aacctgtaag
    58381 ttgaaccgcc acattgttaa acattagaat ttttccctca agagtagtta ttgcaagggc
    58441 aaatggaaag gaatcaaaaa tagaccgtaa gttctgatcg gagaatacaa actctgtcga
    58501 gaaaagtatg gcttgtttat tcatgataag ttaaccccat aaggaactga atgttttaaa
    58561 acagagactt aatttggccg atgactgagc ctcagaacat agcttcttcg aggaaatacg
    58621 gatcagtata aaggttatga tttaagtttt aataggcaac aaggtgtaag aaaattggtg
    58681 aatttgaaga ttttcccgtt acctgcatct ttggcggcgg gttactattg cggcttcaaa
    58741 accggcctca atagctttat ctaattccct gcaagatctt ctaagatcat ttaatgcgaa
    58801 atagttctct gccctgtaaa aataatcctc tccatggcca ggtctgaaaa ggatggccca
    58861 attaaaatct tttatggcat cagaatggtt tcccaatttg gtattggcaa tgcctcttcc
    58921 cctgtaaacg tggtaataga ggtctggcat ttttaccgga ttaattacca gttcatatgc
    58981 ccggccataa gcagcttcaa gaagtccacg tattttatcc cgtgccctgt caaaaaattc
    59041 aagtgctttt tcaggctcat ccagacgatt tgtgtagaga ttggcaagtt caagattaag
    59101 gtttatgttt tcactatcac gcgccagaac gtaattaata gtttccgcgg ctttctcata
    59161 ttcctccagg ttttcataag actgtgccaa cttgaaatga acatcaagat tcaaaaggta
    59221 gggttcttca taatctctgg ccactgtaaa caaaaaggcg gctcttgaaa agtctttcct
    59281 tcgaaactgt tggtcggcag caactttgac ctttgtcaga agtttatttt tttgatcttt
    59341 gagctcaaat tctccgggaa attcttcaat catatatcta agcgtgtcaa aggcgggtcg
    59401 aatcttccca ttttcaaaag tcattattgc catttgcatt cgttgatttc gccaggcatc
    59461 aatccgttcc ttttctttcc gcaacctgta ttcataggca gcctgaacgc ttagtactat
    59521 tatcgcaaca acaaaaacaa acccgaatgc cagtaattga tttcttacat aactccagcc
    59581 gaactgatat tgatactttc ccggaggtga gtagggcctc ctctggtagg tattatatct
    59641 tgggtaattt gtggtttggg actggaatga cttactggtt cgagagtaag ctaaaaccag
    59701 atcatgccgg gcttttttct caggatcaga gagaacatca taagccaggc ttatttcttt
    59761 gaatatttct tcagcacgct tatcgccggg gtttttatca ggatgatacc ggaccgcgag
    59821 tcttttaaaa gtaattttga tttgttctgc ggttgcagac cttggaatac ccagaacctg
    59881 ataataatcg ttcacaatgc taatttaaga agattaccac caatgttgaa cgaataaata
    59941 ccacaatttg atatgcaagg agttaataaa gggttgaatg gaatgaaaat tggtatattt
    60001 atcaacacgc taaatcttca tctgatggcc aattttatca ttccccgatc atggcaaaga
    60061 atccgggaaa gaataactga tgaatcaatt taccaaaatc gcagggagat acttaagaaa
    60121 ttgggtctgg ccggtggcgg attgcttatg tcaccctttc tgtttccatc ctgcacaagc
    60181 tcaaacggga gcgcttcgga atcagtgagt caggcagaca ataatttttc atttcccggt
    60241 atgatggatt tgtatccacc gaaaaagaat gagacctttc ctttagacag gcccataacc
    60301 gatgaatatc tggccactca tcagaataac ttttatgaat tcatacatcc taatgaccca
    60361 aatatatata atgcgtataa atacacccag gattttgaca ctcgcgactg gagttttgag
    60421 gtttcagggc tggctggaaa tactggtaaa tttgctttgg aagaaatgat taagaagttt
    60481 gggctggagg agcgaaccta ccggtttaga tgtgtggaag cctggtcgat ggcagttcca
    60541 tggacgggct tttcttttgc aagtctggtt aaatttctta aacccgaaag cagcgccagg
    60601 tatatcaggt ttttgtctgc cgcggatgaa aatcaaatgc cgggagttaa gaaccaaccg
    60661 tggtacagtt ggccatattt tgaaggactc cggatggatg aggctatgaa tgagttggca
    60721 tttatggcca ccggaattta tggaaagccg ctccctaagc aaaatggtgc gcctatgagg
    60781 cttttagtgc cgtggaagta tgggtataag aatgcaaaat caattgtcaa gattgagttc
    60841 atcagaggtg aacccgaaac tttttggcat aaagttgctc caagtgaata cggattttat
    60901 tccaatgtaa accctggtgt accccatcca cgttggtctc aggctcagga acaaatgttg
    60961 ggtgaagata ctaagcgacc aaccttgcta tataatggct atggggaatg ggtggcccaa
    61021 atgtattctt aatcagtaaa attacctttg acagtttgta tagttgaaac ttcacctgga
    61081 acagtccagg caaaaatcaa tgattccttg tctctggtca ttcttggaaa gccacttgat
    61141 cgggatttgt ctgtttcatt gattggaatg acttctccca atgtaccgtc tctgaaaatt
    61201 ttgcgacaat agataaatgc agtttcgcca ttttcttcca tccaggttac ggcggtaaaa
    61261 ttgtcgaatt gaatcagatc aactctccct aatggattac cgagatcaat tcgcaccggt
    61321 tcgccaaatt gatctccgtc attcccggaa aaagaaacca atacttttct ggttccccgg
    61381 gctgcagtaa accaggcaac tgaaatattg cttccggatg catctacagc cgggccattg
    61441 accggacagc cggggatttc ccattcatca gggtgaacaa tccagggttc tttccactcg
    61501 ccattttcaa accgggagat aaatatgtct cggacttcca ttaatcctct gtcgcgataa
    61561 acgatgaccg ggccgcgaga tgaaatggct ccatcagtct gacaacaatc gcagaccctg
    61621 gaatcaatta catgttcttc gagtttattt ccatctaaat caaaaagacc acacctcaat
    61681 gacatttcct tagttgccgg tttatgttca gaatccacat aatttcttcc atctaaccaa
    61741 acagccagat aattttccct gctcattggc aacaatgtaa cgaaaccatg ttcggtcggg
    61801 gtattatcgt cgtgggccat caccggatta ttccagtctt tgccggtttt gcttgaagca
    61861 agattaacgc cgtaggaata tttaccttca gaaatatatg caaggaagtg agcggcatat
    61921 ccactttcaa gtgcaatcat tgatggaaaa tctgcccaat ttacaaacca gttagcgcca
    61981 gacgcgattt gagtttcgct tacccaattg ccattattta accgggcata tttgagagtt
    62041 gaattatcat tctgatcttc aatccaagag agataaatag taccatccgg accgacggtt
    62101 agatttggtt ccgacgcacg gttgcctgga aatcttatct cttctaaaat catgggtgcg
    62161 gactcaactg ctgaattttc ttcatgtttt gaattacatg agcaaattga aatcaatatg
    62221 attcctaaag cgagtttttt cataatttaa gtgtagctat ataaatgtac tagtgtagat
    62281 ttaaatgaac aaatttgagg gcgaagcttt ttatctgatc ccgcacttcc cggaatttct
    62341 gaaggacatt tttttcatct cccttaaaag ctgccggatc ttcgaaattt tgatgtatgg
    62401 tttttacttc agatggaaaa accgggcagg attccctggc attgtcgcaa acagttatca
    62461 cgaaatcaaa ttttgattct tccaaatcat cgattgtctt tgatgtatga gtggatatat
    62521 caacttcatc ttcattcatt acttttacgg catttttatt caacccatgc gcttcgattc
    62581 cagcactaac gattttagca ttattaccgg caaattttct gaaataacct tcagccattt
    62641 ggctccggca agaatttcct gtacaaagga taaggatttt tttcattagt taaactcttc
    62701 tgatttactc cttcgttcca tcaatatcgt atttctccac tcattttcca attt
//
LOCUS       DASWHX010000184         8545 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_2793868, whole genome shotgun sequence.
ACCESSION   DASWHX010000184 DASWHX010000000
VERSION     DASWHX010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 8545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8545
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2793868"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            221..1663
                     /locus_tag="VGA21_12150"
     CDS             221..1663
                     /locus_tag="VGA21_12150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009579780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651306.1"
                     /translation="MAKLKNIIKQLTNEDYQAIHTSLQESSADKSSYLLKSLRERQLS
                     DNKIMDELGVNTNAYYTLRSRLNQKIEEYLLQQMESPRTDILKKVANINEIIFTKKKA
                     IAIATLKKLEKELLDYDLSNELTIVYKSLKKLHINAPDYFNYSQSYNKHVAYMLALDK
                     AEDLLAQYLQKYGNYTLTGSEIEKMGLTVLAEEMDNVCRLYQSHRLYVYKSCMMVFHK
                     LFVLKDEEAGVDEEPVEDALDKVEQIFESYNLDPIYYHLHLAFEFLKLEYYNHYKVYR
                     KAEKYFEEVNDATSSLLSNYSLFIYPARFLISKIERSMRLGTEGELYSESEGLFLGYE
                     PDKDDVPKYITYVIYRALCCYYAEKYDESARWINNLLNEMSLKKFPELLLEIKMILAL
                     QYCMMHDFDLFSQLINSVQRQLRLIGKVGFNHIIILTKILRTSMNEGKKNKEAKIKSL
                     IGRIGKINVPYFVPTSLIKMDEALVSALSR"
     gene            complement(1664..2080)
                     /locus_tag="VGA21_12155"
     CDS             complement(1664..2080)
                     /locus_tag="VGA21_12155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009579779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9651307.1"
                     /translation="MEETKLKFYAVMLIDDNEIDNLINQKMIEAANISEHIYTHTGAR
                     SAIEFLRNLEKLENESKKVLPEVIFLDIDMPLMDGFQFLDEFDKLSESTKSNCKVVML
                     TSSINPQDVSKSKKYSYVKKYINKPLSQENLLNLKI"
     gene            complement(2203..3735)
                     /locus_tag="VGA21_12160"
     CDS             complement(2203..3735)
                     /locus_tag="VGA21_12160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009579778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX9651308.1"
                     /translation="MTTSEILNHLGLSKQNSGVSKGQTWLSGTGELIESYSPVDGKLI
                     GTVTSAGREDYEQVINQAQKAFAEWRLIPAPRRGEIVRQIGLALRENKIALSTLVSYE
                     MGKSLQEGFGEVQEMIDICDYAVGLSRQLHGLTMHSERLNHRMYEQYHPLGIVGIITA
                     FNFPVAVWAWNSMIAWVCGDVCVWKPSEKVPLCSIACQNIINKVFVANNVPEGVSGIL
                     NGKIEVGELLTKDTRIPMISATGSIRMGKAVGATVGARLGRALLELGGNNAIIITENA
                     DIDMTIRGTVFGAVGTAGQRCTSTRRLIIHEKIYDFVKEKLVKAYKQIKVGNPLDERN
                     HVGPLIDRAAVKMYSEALEKVKAQNGTFAVEGGVMDGAGFESGCYVRPAIAEIGKRAE
                     IIQHETFAPILYLIRYKDLDEALEIHNGVVQGLSSAIMTLNMREAETFLSVKGSDCGI
                     ANVNIGTSGAEIGGAFGGEKQTGGGRESGSDAWKAYMRRQTNTINFGSELPLAQGIKF
                     DV"
     gene            complement(3819..5270)
                     /locus_tag="VGA21_12165"
     CDS             complement(3819..5270)
                     /locus_tag="VGA21_12165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017733099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX9651309.1"
                     /translation="MTRRQFFLTVLFASFFGAMVAFAIFLSLSPNDGENFLNTQSDGF
                     SISEKQSMKTANANRPAINYEVPATLNFIDAADEVISGVVHIQSIYSKGSVTDLLNPY
                     RRGPAVSSGSGVILTVDGYIATNNHVIEDASEVRVTLFNNRSYLAKVIGSDPQTDLAL
                     LKIQADNLDFIPYGNSDEVAIGEWVLAVGNPFNLNSTVTAGIVSAKARNIQIFNENGL
                     EIESFIQTDAAVNPGNSGGALVNLKGELIGVNTAIATQTGSYEGYSFAVPVSLVRKVM
                     DDLLEFGNVQRGLLGIIIRDVDARLAEVEGLSMVTGVYVPAVNPSSAAEEAGIQSGDV
                     IIEINNIEVKNSSELQELVARHRPGDEILVTYMRKDQRYEVKAQLRNFVGNTELDYRP
                     TSSTISGAHFEDVPKSEQVLLGISGGARLKQIEEGKWTDTGIEKGFVITSIDKINVDD
                     AEDLRRIMSNKSGGILVEGINSDGDSKLFALNW"
     gene            complement(5497..5940)
                     /locus_tag="VGA21_12170"
     CDS             complement(5497..5940)
                     /locus_tag="VGA21_12170"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEX9651310.1"
                     /translation="MTLIKYRNPMETYPASFSRMIDNLFNDKWLNDSPEDFNLRTFNP
                     KVDIAEVENEYEVHLTVPGMKKDDFKIEVNENTLTISGERKFENEEKAKQFHSIESHY
                     GKFARSFQLPKNVNQDKIKANYNDGILVVKLAKTEKSLEKVIKIQ"
     gene            6159..7415
                     /locus_tag="VGA21_12175"
     CDS             6159..7415
                     /locus_tag="VGA21_12175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009581200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEX9651311.1"
                     /translation="MALGPGILFASTCIGVSHLVQSTRAGADYGFALLIAIVVANVLK
                     YPFFEFASRYTSATGVNILDGYLKEGRWILILYFLISLPSMFIVTAAVTFVTAGLLGN
                     LLGLNLTTDIWSALLLGFCVIALGIGKYKLLDSLLKWVGAILLISVVAAFFSALINGK
                     AAMVDGFVAPSVLEPAGIIFLIALMGWMPTAVDISTWTSLWAEEKAKVTGNTPSLKEA
                     SFDFNFGYWVSAILAICFMTLGAYVMHGTGTELSNNSTVFAGQVISLFTESIGNWSYI
                     IISIAAFSTMFSTTIAVVDGYGRAISRTTQLLFSLESSSRRPFIIWTIIISIGAFLVI
                     TIFVGNLKQLVDFATILSFVIAPLAGFINYRVIFSKEIAKEFVPPAWLKWLAIAGLIF
                     LSVFTIIYFFVLLDPEGTSQFFKNIL"
     gene            complement(7417..>8545)
                     /locus_tag="VGA21_12180"
     CDS             complement(7417..>8545)
                     /locus_tag="VGA21_12180"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX9651312.1"
                     /translation="AGDKINENWRLYGRSTSDDKAPIIALLAAIDALQNSEIAFTSNI
                     KLLFDGEEEMGSPHVMDYLTNFNSLFEDIDVWLMVDGPVFLTGQPVLNFGSRGITGME
                     LTVYGATRPLHSGHYGNYAPVPGQMLAALLATMKDEKGKVLIEGYYDTVEPISDFERE
                     QINKIPKIDEQIRTDLGIAQPEGDGETIYKRLLLPSLTIRGLSSGNVGALARNVIPNI
                     AVATLGMRLVKGNDPEQMMNLVEAHIKKQGWHIVYDEPDMETRMNYPRIIKVEREKDG
                     GPASKISMNHPAVLPVIESIKSFTGDNLVLIPSSGGSNNAKVAVLDGIKMPGISISIA
                     NHDNNQHAENENIRIGNLWYGIDLMGVLLTMPEPKTELKKK"
BASE COUNT         2377 a         1854 c         1590 g         2724 t
ORIGIN      
        1 tgggtttgtt aaagttaaaa gaaattattc aaagataaaa tatacctttt ccgaatttca
       61 ctaaaccaga atggacgata accagaaatt tgctgtagaa attcatgtta ttttcaccaa
      121 aaacttacct ttagccaacc ccgggtatat tttttaatta aagctattca atatcacttg
      181 ggatactctt aaatttccgg acattttccc agcccaaata atggcaaagt taaagaacat
      241 aatcaagcaa cttactaacg aggattacca ggctattcat acatctcttc aggaaagcag
      301 tgcggataaa tcaagctacc tactgaaatc actgcgtgag cgacagttgt cagacaacaa
      361 aatcatggat gagttgggag ttaatactaa cgcttattat accctgcgat cccggcttaa
      421 ccagaaaatc gaagaatact tgcttcaaca gatggaaagc ccaaggacgg atatccttaa
      481 gaaggtggct aatatcaatg aaataatttt cacaaagaag aaagctattg caatcgcaac
      541 attaaagaag ttagaaaagg aattgcttga ttatgatttg tccaatgagc tgactatcgt
      601 ttataagtca cttaaaaagc tgcatattaa tgctccggat tatttcaatt attcgcaatc
      661 ttataacaag catgttgcct atatgctggc tttagacaaa gctgaggatt tgcttgctca
      721 atatttgcaa aagtatggta attatacgct tactggaagt gagattgaga agatgggtct
      781 gacagtttta gctgaggaaa tggataatgt gtgtagatta taccagtcgc atcgacttta
      841 tgtttacaaa agttgtatga tggtatttca caaacttttt gtcctgaaag atgaagaggc
      901 gggtgtggac gaggaaccgg tagaggatgc cctggataag gtggagcaaa ttttcgagtc
      961 atataacctt gaccccattt attaccatct ccacctcgcg ttcgaatttc taaaacttga
     1021 atattataat cattataaag tatatcgaaa agctgaaaaa tatttcgagg aagtgaatga
     1081 tgcaaccagc agtttattat caaactattc cctgttcatt tatccggcac ggttccttat
     1141 ttctaaaatt gaaaggagca tgagacttgg gacagaaggt gaactgtatt cagaaagcga
     1201 ggggctgttc ttaggttatg agcctgacaa agatgatgtt cctaaatata taacatacgt
     1261 catctaccga gctttgtgtt gttactatgc ggaaaaatat gatgagtccg cacgctggat
     1321 taataacctg cttaatgaga tgagcctgaa aaaatttccg gagcttctgc tcgaaattaa
     1381 aatgatcctt gctttgcaat attgcatgat gcacgacttt gatttattta gtcagttaat
     1441 aaacagtgta cagagacagc tgcgactcat tggaaaagta ggatttaacc acattatcat
     1501 tcttaccaag attctgcgta cttctatgaa tgaaggcaaa aagaataaag aagctaagat
     1561 aaagtctctg attggaagga ttggtaaaat aaatgttcct tattttgttc caacttcctt
     1621 gatcaaaatg gatgaggcgc ttgtcagcgc tctgagcagg taatcagatt ttcaagttca
     1681 acagattttc ctgtgaaaga ggtttattga tatacttttt cacgtaggaa tattttttgg
     1741 atttactaac atcctgcgga tttatggaag aagtcagcat aaccaccttg cagttacttt
     1801 ttgtagactc tgaaagctta tcaaattcat ccagaaactg gaatccatcc atcaggggca
     1861 tatcgatatc aagaaaaatt acttccggta aaaccttctt ggattcattc tccaactttt
     1921 ccagatttct caggaattca attgcgcttc gtgcacctgt atgtgtataa atatgttctg
     1981 atatattcgc agcttcaatc atcttttgat taataagatt atcaatctca ttatcatcaa
     2041 tcagcattac agcataaaat tttaacttag tttcctccat ttctaaatga acttattccc
     2101 aatttatcct taaatattat aattaaaacc cttattaaca aaaactaaat tcgcaattgt
     2161 tttctttatg taacaaacaa actaaaattt gatgttttca aatcacacat caaacttaat
     2221 tccttgtgcc agaggtaatt ccgaaccaaa attgatggta ttagtctgcc ttctcatgta
     2281 tgctttccag gcatcagagc ctgattccct accacctcca gtctgttttt cgccgccaaa
     2341 ggcaccgcca atctcagcac ctgaagttcc aatatttaca ttagctatcc cgcagtcaga
     2401 accctttact gaaagaaacg tttccgcttc ccgcatattg agtgtcataa ttgccgacga
     2461 caatccttgc accactccgt tgtgtatttc caacgcttca tccaaatctt tatacctgat
     2521 taagtataaa atcggcgcaa atgtttcatg ctgaattatt tctgcacgct tcccaatttc
     2581 agcgatagca ggtcttacat agcatcctga ttcaaatccc gcaccgtcca taacgccacc
     2641 ctccacagca aaggttccgt tttgcgcctt gaccttttcc agggcttctg aatacatttt
     2701 tactgccgcc cggtcaatca atggtcctac atggtttctt tcatcaagag gattgcctac
     2761 tttaatttgc ttgtaggctt ttactaattt ctcttttaca aaatcataga ttttttcatg
     2821 gataatcaat cttcgcgtag acgtacaccg ttgcccggca gtacccaccg ctccaaacac
     2881 cgtacccctt attgtcatgt caatgtcggc attttctgtg atgataatcg cgttgttgcc
     2941 accaagttcg agcaaagccc gaccgagtct cgctcctact gttgctccca ctgcctttcc
     3001 catccgtatc gaaccggtcg cagaaatcat gggaatccgg gtatctttgg ttagaagttc
     3061 acccacttca attttaccat ttaaaatacc tgaaaccccc tccggaacat tgttggcaac
     3121 gaacacttta ttaattatgt tttggcatgc tatactgcaa agtggtactt tttccgaagg
     3181 tttccacaca caaacatctc cgcaaaccca tgcaatcatg gagttccatg cccacaccgc
     3241 gacagggaaa ttaaatgctg taattattcc aactataccc aaaggatggt actgctcata
     3301 cattctgtga ttcaatcgtt ctgaatgcat ggttaatcca tgcaactgcc gtgacaagcc
     3361 gaccgcgtag tcacagatat caatcatttc ctgaacttca ccaaatcctt cctgtaagga
     3421 tttacccatt tcataggata cgagtgtgct cagtgctatt ttattttccc gcaaagctaa
     3481 tcctatttgt cttacgatct ctcccctccg gggtgccgga atcaatcgcc actccgcgaa
     3541 tgctttttga gcctgattta ttacttgttc gtaatcctcc ctaccggcag atgtcactgt
     3601 ccctataagt ttgccatcaa ccggagaata cgattcgatt aattccccag ttccgcttaa
     3661 ccaggtttgg cctttggaaa ctccgctatt ttgcttactt aatccaaggt gatttaagat
     3721 ttctgaggtt gtcatttgca agttgattta gacttgcaaa actacggaat tgccttctta
     3781 ttgattgagt gtttccgaaa agaaaaagcg ggtaaggatt accaattcag cgcgaaaagt
     3841 tttgaatcac catccgaatt gattccctcc accaggattc cccctgattt atttgacatt
     3901 attcggcgca ggtcttcggc atcatcgaca ttaattttat caatcgaagt aataacaaat
     3961 cctttttcaa ttcccgtgtc tgtccatttt ccttcttcga tctgctttaa ccttgctcct
     4021 ccgctgatac cgagcagtac ctgttcactt ttcggaacat cttcaaagtg agctcccgaa
     4081 atggtggaag aggtggggcg ataatccaat tcagtattgc caacaaaatt tcttagttgt
     4141 gccttcactt catatcgctg atcctttctc atataggtaa ccaaaatctc atctcctggc
     4201 cggtgacgtg ctaccaactc ctgaagttca gagctgtttt tcacttcaat attattaatt
     4261 tcaattataa cgtctccact ttgaattccg gcttcttcgg cagcactgga aggattaaca
     4321 gcaggtacat atacgccagt gaccatgctt agtccttcaa cttccgccag ccttgcatcc
     4381 acatccctta tgattattcc caggaggcct ctttgtacat taccaaactc caataggtca
     4441 tccatgacct tacgtaccaa tgaaacagga acggcgaatg aataaccttc ataggacccg
     4501 gtttgtgtag ctatagcggt attcactcca attagctctc ccttgaggtt aacaagggca
     4561 cctccgctat ttccagggtt tactgctgca tctgtctgaa taaaagattc tatctctaat
     4621 ccattttcat taaatatctg aatgttcctg gcttttgcac tcacaatgcc tgccgttacc
     4681 gtagagttca ggttgaatgg atttcccacc gccaacaccc attccccaat agccacttca
     4741 tcagaatttc cgtaaggaat aaaatccaga ttgtctgcct ggattttcaa taaggccaga
     4801 tctgtttggg gatcacttcc aattactttg gcaagatatg accggttatt aaacaaggtg
     4861 accctgactt cactggcatc ctcaattaca tgattattgg ttgctatata tccatcaaca
     4921 gtaaggataa ccccggatcc tgaagacact gcaggacctc tgcgatatgg atttagcaga
     4981 tctgtgactg agccttttga ataaatagat tgaatatgaa caacaccgct tattacttca
     5041 tcggctgcat caataaaatt taatgtagca ggcacttcat aattaattgc tggccggttc
     5101 gcattggcag tcttcatgga ttgcttttca gaaatactaa acccatcgga ttgggtattc
     5161 aggaaattct ctccatcgtt gggagataaa gaaagaaata tggcgaaagc caccatagcg
     5221 ccaaaaaagg aagcaaatag cacggtaagg aagaattgtc tgcgagtcat aattctgtca
     5281 tttatttatc ccccaataaa aacttatacg caaatagaaa tggtacgttc cactaaaata
     5341 gctcagaatt tataaatttc ggacaaaatg gggcatgaaa ggcaaatcca tgggaattaa
     5401 atgaatccac aaacaaatga aaatccagag ggttcttaaa acaaaaaagg cgacactagc
     5461 gtatcacctt ttttccttca atcaccacta tcagctctac tgaattttaa ttactttctc
     5521 caggcttttt tctgtttttg caagtttcac caccaaaatt ccatcattgt aatttgcttt
     5581 gattttatcc tggtttacat tttttggaag ttgaaatgaa cgggcaaatt taccatagtg
     5641 gctttcgatt gagtgaaatt gctttgcctt ttcttcattc tcaaattttc tctctcctga
     5701 aatggtcagg gtattttcat tgacttcaat tttgaagtca tccttcttca ttccgggaac
     5761 tgtcaaatga acttcatatt cattctcaac ttccgcgata tcaacctttg gattaaatgt
     5821 tctaaggttg aaatcttcgg gtgaatcatt tagccatttg tcgttaaaca ggttgtcaat
     5881 catcctgcta aatgaagcag ggtacgtctc cattggattt ctgtatttta ttagtgtcat
     5941 agctgtaatt agttttagtt ttactttttg ttcccctcat ttcaaatcca ataccaaccc
     6001 aaaaaagtgt gctgaaagta actttttcac gtcatattgt cacaagttat caagaaagtt
     6061 taaattttac tgcctttttg tctgctattt gaggaattag ggttttggtt atctttaggg
     6121 tttttacccg gatgaataca aaaatcagga gatacttaat ggcccttggt ccagggattt
     6181 tgtttgcaag tacctgcatt ggagtttcac atttggttca atctacgcgg gccggcgctg
     6241 attatggatt cgcgcttttg attgcgatcg tagtcgccaa cgtgttaaag tatccttttt
     6301 tcgaatttgc gtcgcggtat acatcagcaa ccggagttaa cattctggat ggttatttaa
     6361 aggaaggccg atggatcctg atactttatt tcttaatctc attgccctcc atgtttattg
     6421 ttaccgcggc ggtaacattt gttactgcag gattgcttgg aaatcttttg ggtctaaacc
     6481 tgactactga tatttggtcg gcactgttac tgggattttg tgtaatagct ctgggcatag
     6541 gtaaatacaa gctattggat tcgctgttaa agtgggttgg cgccattttg ctaatttcag
     6601 tggtcgcagc atttttcagt gctttgataa atggaaaagc ggcaatggtt gatggtttcg
     6661 ttgcaccttc tgtactggaa cccgccggaa tcattttcct cattgcccta atgggctgga
     6721 tgcccacggc cgtggacatc tctacctgga caagtttatg ggctgaagaa aaagccaaag
     6781 tcactggcaa tacaccaagt cttaaagaag catctttcga tttcaatttt ggatattggg
     6841 tatcagcaat tctggccatt tgtttcatga ctttaggcgc atacgttatg catggaaccg
     6901 ggacagaact ttccaacaat tcaacggtat ttgcaggcca ggtaatttca ttatttactg
     6961 agtcaatcgg taactggagt tatatcataa tttcgatagc cgctttttcg acgatgttca
     7021 gtacaacgat agcggtagtg gatggatatg gaagggcaat ttcccgcact actcagcttt
     7081 tgttcagcct ggagagttcc tcgcgccgac cttttattat ctggacgatt attatttcga
     7141 ttggcgcttt tttggtgatc acaatttttg taggcaacct gaaacaactg gttgactttg
     7201 ccacaatcct ttcttttgtg attgcgcccc tggcaggatt tattaattat cgggttattt
     7261 tctccaaaga gattgctaaa gaatttgtcc cgcctgcatg gttaaagtgg ctggctatag
     7321 caggacttat ttttttatct gtatttacta ttatttattt ctttgtattg ctggaccccg
     7381 aaggaaccag ccagttcttc aaaaatattc tataagttac ttcttcttaa gttccgtttt
     7441 cggctcaggc atggtgagta agactcccat aagatctatt ccataccata agttaccaat
     7501 ccggatattt tcgttttcgg catgttgatt attgtcatgg ttagcaatac tgatgctgat
     7561 gccaggcatc ttgataccat ccagcacagc aactttcgca ttgtttgagc cgccgctact
     7621 gggaattaat acgaggttat cgccggtgaa agattttatt gattcaatca caggcaagac
     7681 tgccgggtga ttcatggaga tttttgatgc tggaccgccg tccttttctc tttccacctt
     7741 aattatcctg ggataattca ttcgggtctc catatccggc tcatcgtata caatgtgcca
     7801 gccttgtttc ttaatatgcg cttccaccag gttcatcatt tgctccggat catttccttt
     7861 taccaaccgc atacccaggg ttgccaccgc tatgttaggg ataacatttc tggccaaggc
     7921 accaacattg ccactgctta aaccccggat ggttagggaa ggcaatagca ggcgtttata
     7981 aattgtttca ccatctcctt ccggttgagc tattcccagg tctgtacgaa tttgttcatc
     8041 aattttcgga atcttattta tttgctcccg ttcaaaatcc gaaataggtt caaccgtatc
     8101 ataatatccc tcaatcaaaa ctttcccttt ttcatctttc atggttgcta acagagctgc
     8161 cagcatttgt ccgggaaccg gtgcatagtt tccgtaatga ccactatgga gggggcgtgt
     8221 tgctccgtat actgttagtt ccattccagt aattcccctt gacccaaaat tcagaacagg
     8281 ctggccggtt agaaataccg gaccatcaac catcagccag acatcaatat cttcaaacaa
     8341 tgaattgaaa ttggtaaggt aatccattac atggggggaa cccatttctt cttccccatc
     8401 aaataaaagt ttgatatttg aagtgaaagc tatttcagaa ttttggagcg catctattgc
     8461 tgccaataat gcaattatgg gagctttatc atcactggtc gaccggccgt atagtctcca
     8521 gttctcgttt atcttgtctc cggct
//
LOCUS       DASWHX010000185         2966 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_2067042, whole genome shotgun sequence.
ACCESSION   DASWHX010000185 DASWHX010000000
VERSION     DASWHX010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 2966)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2966)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2966
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2067042"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..561)
                     /locus_tag="VGA21_12185"
     CDS             complement(<1..561)
                     /locus_tag="VGA21_12185"
                     /inference="COORDINATES: protein motif:HMM:NF026253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HEX9651313.1"
                     /translation="MYLKILSLLLLFGCVTVHAHGDNDFVIIQENNKKGLATIEGKVL
                     IPPTYEDIGWSQGILDISHEVIGYKQNESWGLITIHNKVITPALYSELFKANDHVFIA
                     AKKGQFSQKNFFGLLDHQGKNLSGFQFTDLAGAGNRIIGIYRDGANYYYGVLGVLNNM
                     SNVVIPFSYKKIEKISESLFRVKNEAN"
     gene            891..1484
                     /locus_tag="VGA21_12190"
     CDS             891..1484
                     /locus_tag="VGA21_12190"
                     /inference="COORDINATES: protein motif:HMM:NF014021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEX9651314.1"
                     /translation="MESLGNSIRKLRENKELPLRTVAAYLDIDQAILSKIERGQRKAS
                     RKQVVKLAQYFKVKENELITTWLSDKLVDDLQDEKTALKALELAEERVAYGKYQKINR
                     SAILKKINSYFVKDGRVSKAWVFGSFSRREEDYKSDIDILIEVPPHSKFSLFDLSDIQ
                     YHLEKTLSTKVDLVMREAVRPPLMERMKNELMLVYER"
     gene            1474..1821
                     /locus_tag="VGA21_12195"
     CDS             1474..1821
                     /locus_tag="VGA21_12195"
                     /inference="COORDINATES: protein motif:HMM:NF014043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HepT-like ribonuclease domain-containing
                     protein"
                     /protein_id="HEX9651315.1"
                     /translation="MRDKDITSHDRLGHIQEAMEKIETFIKGHTRESFLKDEVIISAT
                     LFQFAIVGEAIRYLSNEILDKYNYPWHKVRGFRNFILHEYHAISYRVIWNTVKEDLPQ
                     LKKKITEILRKEF"
     gene            2115..>2966
                     /locus_tag="VGA21_12200"
     CDS             2115..>2966
                     /locus_tag="VGA21_12200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009580208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX9651316.1"
                     /translation="MYLEQLYTGCLAEAAYYIESEGEAAIIDPIRETEPYIKLAKSRN
                     AKIKYIFETHFHADFVSGHIDLAAKTGAGIIYGPGAKTAYEAYNAKDGEEFNLGKLKF
                     IVLHTPGHTPESSCYLLFDETGKEHALFTGDTLFINDVGRPDLLDGVMTKEELAGMLY
                     ESLNNKIKVLPENVLVYPAHGPGSACGKNIGPERHSTIGEQKRTNYALQPMSKEEFIK
                     KVTDGILPPPSYFFEDARINKLGYIPLDEVFEGSLKSLSLDNFRNSITENTVILDTRK
                     PNTFEKGF"
BASE COUNT          985 a          558 c          602 g          821 t
ORIGIN      
        1 gttagcctcg ttcttaaccc ggaaaagaga ttctgaaatt ttctcaatct ttttatagct
       61 gaatggaatg acaacgttgc tcatgttatt caaaacgcct aaaacgccat aataataatt
      121 ggctccatct ctgtaaattc cgattatcct gttaccggcg ccggccaaat cggtaaactg
      181 aaatccactg agattttttc cctggtgatc cagcagacca aagaagtttt tttgactgaa
      241 ctgacctttt tttgcagcaa taaaaacatg atcatttgcc ttaaagagtt cagaatacaa
      301 ggcaggtgta ataaccttgt tatggatagt aattaatccc caactttcgt tttgcttata
      361 tcctataact tcatggctaa tatccaaaat accctgcgac cacccgatgt cttcgtatgt
      421 gggaggtatc aaaactttgc cttcgatggt cgccaggcct ttcttattat tttcctgtat
      481 aatgacaaaa tcattgtcac cgtgagcatg cacagtcaca catccaaata gaagtagtaa
      541 ggaaagaatc tttagataca aacggaacaa attttgttga tcaactgttg tcagtaataa
      601 ttagcaaaat taagtacttt tgtatagaaa aatgtatcat aatttgatat ttacaggagt
      661 tagtttcgta tttgaattaa agtgattcct taccggattt tttttgagat tcatcgcgaa
      721 ttctcagtaa accaaaaacc ggagatattt tactaataag aaagtacatt tcgcattttc
      781 aaaaagcttc tgccctaaaa tccccgccgc cctccaggaa ataattaaga tttcctcttg
      841 cttaaataaa agactttgac aaaatttgtc aaaacaaaaa agttcaagaa atggagagtt
      901 tagggaacag tatcagaaag cttcgtgaga ataaagaact acccttgcgg acagttgcgg
      961 cctatcttga tattgaccag gctatactga gtaaaataga gcggggtcaa aggaaagcat
     1021 cacggaaaca ggtagttaag cttgcgcaat attttaaggt gaaagaaaac gaactaatca
     1081 ccacctggct ttctgacaaa ttggtagatg acctgcagga tgaaaagact gccttaaagg
     1141 cgctggaact ggcggaagaa agagtcgcct atggcaaata ccaaaaaata aaccgttccg
     1201 ccatcctaaa aaaaatcaat agttattttg tgaaagacgg gcgcgtgtca aaggcatggg
     1261 tttttggatc tttttcaagg cgcgaagagg attataagag cgatattgat atactgattg
     1321 aggtaccccc tcattcaaaa ttttcgcttt ttgatctttc cgatattcaa tatcatttgg
     1381 agaaaacact tagcaccaaa gtagacctgg tgatgcgcga ggccgtgcgg ccaccgctaa
     1441 tggaaagaat gaaaaatgag ttaatgctcg tctatgagag ataaagacat aacctcacac
     1501 gaccggctgg gacacattca ggaggcaatg gagaaaattg aaacctttat taaaggccac
     1561 acccgcgaat cttttttaaa agacgaggtc attatcagtg ctacgttatt tcagtttgcc
     1621 attgtagggg aagccattcg atatctgagc aacgaaattt tagataagta taactatccc
     1681 tggcacaagg tgagagggtt cagaaacttt attttgcatg aataccatgc catcagctac
     1741 agggtaatat ggaataccgt aaaagaggac ttgcctcaac ttaagaagaa gatcacagaa
     1801 atactgagaa aggaattttg attatgccaa ctctccagtt taaaggaaag acatttgttc
     1861 aaaaccatca ttcagccaca tggtatcatc agcttgttcc tcaaaagaag ttgagtcaat
     1921 accggaaacg aaaattggat ttacaatgac aacgtgaact ggtatttcct gactttatta
     1981 cagcggagat tgaccaggaa gctaagaggg actaagtgag cgcattttct tgaatttggt
     2041 tcactaaact gaatttaaag cgttcataat catgctattt taacttaatt ttgcaggaaa
     2101 tagctcaata acacatgtac ctggaacagc tttacacggg atgcttggca gaggccgctt
     2161 attatattga atcggagggg gaagctgcaa ttatagatcc aatcagggag actgaacctt
     2221 acataaagct ggcaaaatca agaaatgcta aaattaaata catttttgaa acccatttcc
     2281 atgcggattt tgtttccggg catattgatt tggccgccaa aacaggagct ggaataattt
     2341 atggcccagg agccaaaacg gcttatgaag cctacaatgc aaaggatggt gaagagttta
     2401 atttaggcaa gctaaaattc atagtgttac atactcctgg tcatacaccc gaatcaagtt
     2461 gttatctttt attcgatgaa accgggaaag aacatgccct gttcacagga gatacgctat
     2521 ttattaatga tgttggaaga cctgatttac ttgatggcgt aatgacaaaa gaagagttgg
     2581 ccggaatgct gtacgaatca ttaaacaata aaataaaagt tttgcccgaa aatgttctgg
     2641 tatatccggc acacgggccg ggttccgcct gtggcaaaaa tataggtccc gaaaggcatt
     2701 caacaatcgg tgaacaaaag agaacgaatt atgctttgca gcccatgagc aaggaagaat
     2761 tcataaaaaa agtaacggat ggaattctgc ctcctccctc atatttcttt gaggacgccc
     2821 ggattaacaa actggggtat atcccgctcg atgaagtttt cgaaggttcc cttaagtcgc
     2881 tatcattgga taatttcaga aatagcatta cggaaaatac ggtaatcctt gataccagga
     2941 aacctaatac ttttgaaaaa gggttc
//
LOCUS       DASWHX010000186        10492 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_1307504, whole genome shotgun sequence.
ACCESSION   DASWHX010000186 DASWHX010000000
VERSION     DASWHX010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 10492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10492
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1307504"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(172..870)
                     /locus_tag="VGA21_12205"
     CDS             complement(172..870)
                     /locus_tag="VGA21_12205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018475222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HEX9651317.1"
                     /translation="MSKYKELKEKCYEANLQLPRLKLVHYTFGNVSAADRDNGVFAIK
                     PSGVPYADLKPEDIVILNFENKIIDGKLKPSSDTKTHSYLYNAWPEIGGICHTHATYS
                     VAWAQAQADIPILGTTHADHLTHDIPCTPPMKDNLIEGDYEHNTGRQIVDCFDSKKLD
                     YKEVEMVLVGNHGPFTWGKDSGKAVYNSKVLEELAKMALLTLSINPDAPRLSDALIKK
                     HYERKHGKNSYYGQ"
     gene            complement(879..2525)
                     /locus_tag="VGA21_12210"
     CDS             complement(879..2525)
                     /locus_tag="VGA21_12210"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014021528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HEX9651318.1"
                     /translation="MEINYVIGLDFGTDSVRAVLVNAGNGETLSTKEHWYKRWAAGKY
                     CDASKSQFRQHILDHLEGIEETIAGVVKDSGVEPEFVKGICIDTTGSSPVPVDESGTA
                     LCLKPEFENNPNAMMVLWKDHTANKEAEEINRLARSWGGIDYTKYEGGIYSSEWFWAK
                     ILHIIRKDEKIREAAFSWVEHCDLMTYILTGTSDLKSFKRSRCAAGHKAMWHESWGGL
                     PDEKFLMQLHPTFKKLKGNLYAQSFTSDHVAGNLSDEWASKLGLTPATIIAVGTFDAH
                     AGAVGAEVEEFALVRVMGTSTCDIIVSPGEVVDNQTVKGICGQVNGSVIPDMVGLEAG
                     QSAFGDVLAWFRDVLSWPVENLNLRLEAEDKLKLKDSIITGLSAQAEKIPLNEMLPIA
                     LDWINGRRTPDANQALKSAIMNLNLATKAPEIFKALVESICFGSKKIVERFSDEGVEI
                     RTVIGVGGVAKKSPFVMQTLANVLNMPIKVASSDQVPALGAAMYAATAAGIFKNVNEA
                     IKTMGNGFEKTYNPDPEKMEIYLKLYEKYCQLGEFVESQT"
     gene            complement(2665..3975)
                     /gene="chrA"
                     /locus_tag="VGA21_12215"
     CDS             complement(2665..3975)
                     /gene="chrA"
                     /locus_tag="VGA21_12215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HEX9651319.1"
                     /translation="MQKPSFKEAFWFWLKLGFISFGGPAGQIAIMHEFLVEKKRWISN
                     SRFLHALNYTMILPGPEAQQLATYTGWLLHGTKGGLIAGIFFVLPSMFILLALTVLYA
                     KYGDIPWVYALFEGLKPAVLAIVILALIKIGKKSLHTFFHYLVGLGAFICIFFFNISF
                     PIIILAALLIAFAVQKFYPNLFVKSQTAINQSIKDESGYFINNEHKSEVKPHVWLLVR
                     QLTIVAMLWFIPLIIFYLVDINFYFWKQLVLFFTQAALVTFGGAYAVLPYVAQVSVEK
                     LHWLSELQMIDGLALGETTPGPLVMVLVFVGFMAAYNNQNGSLIAGSFGLVTTAFYTF
                     LPCFLFIFAGAPIIERTRDNRMIREALSIVTSAVVGVVLNLTLYLGKAVIFPNEFIFL
                     QIDFISIGWIAISFIAMYQFRVGMIPWIAISAAFGLVNYFLSNI"
     gene            complement(3998..4993)
                     /locus_tag="VGA21_12220"
     CDS             complement(3998..4993)
                     /locus_tag="VGA21_12220"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HEX9651320.1"
                     /translation="MNDNLLILLSRTNVKELLTIGEGISAVENAFKLYGMGKATPPGI
                     LGMHADAGGFHIKAGLMNLANHYFVAKTNANFPENRKNFNLPTIQGVVLVMDAKNGRP
                     LSIMDSIELTIIRTGAATAVAAKYLSKPDSKIVTICGCGEQGKISIRMLREIRDIQKV
                     YAYDTNHQTAEIFAKDVRDTLSIDVELTDSIEKVIPLSDICVTCTTSEKAFVKEAYIY
                     PGLFLAAVGADNEEKQELEPEVLKKSKLVTDLTAQASKIGELHHAIEAGLVDEDFVYA
                     ELGEIVAGTKQGRKSPDEIFVFDSTGMALQDVASAVIVYEKALSLRKGISFDFSK"
     gene            complement(5084..5938)
                     /locus_tag="VGA21_12225"
     CDS             complement(5084..5938)
                     /locus_tag="VGA21_12225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013928483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate resistance protein ChrB
                     domain-containing protein"
                     /protein_id="HEX9651321.1"
                     /translation="MQWITRESPKIDRIACPWLIKKFIDHDAVFHFVPFKDVKSSAKI
                     LNAIPYDIPGVEYTHYDDQCTFDYFINKHKIEDPAVAIIAKIVRGADTDRHSLASQAS
                     GLWAISSGLAFNIKKDHELLEAGFIIYDALYSWAKHLKDLKHTQNPTEILLVDVYRKF
                     LKHKNSEKKIPKWVEELKEIIQDQIDTELSLSLKEVSENLQVHPAYLSREFSKYFEDL
                     NFGDYLRKLRIEKALKLLETTKYSLSEIAFLCGFSDQSHFTRIFKKHLGIPPMAYKKN
                     KLRESRRK"
     gene            complement(5942..6520)
                     /locus_tag="VGA21_12230"
     CDS             complement(5942..6520)
                     /locus_tag="VGA21_12230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002978432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX9651322.1"
                     /translation="MTTLYIKNMVCNRCIMVVKQEIEKQGLHPQKISLGEVTIQEDDI
                     SGVTKDNLDAALRNLGFERIDDKKARIIEAIKNKIIQMIHHSDNVELKVNWSNLLSEE
                     LKYEYNYLSSLFSSVQGITLEQYIIRQKVEKVKEYLFYDEFNLSEIADRLGYSSVSHL
                     SAQFKKITGFTPSEMKKSRGIDNNRKPIDSVS"
     gene            complement(6698..8536)
                     /locus_tag="VGA21_12235"
     CDS             complement(6698..8536)
                     /locus_tag="VGA21_12235"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX9651323.1"
                     /translation="MKILDKIKSKVTYTNLVILIAGILIGWLIFGGNQKPSENLAADH
                     TLEDHEAGQVWTCSMHPQIREDGPGKCPICGMDLVPVEMDETDLDEEVSQFTVKLSNA
                     AIKIAEVSTTVIERKAPYNEIYLPGKVMPDERRISQLTSRFAGRIEKLSVNFTGQKVR
                     EGQVLAKIYSPELVTAQKELFEAVKYKESNPRYYEASRQKLKLWSLTEQQINNIEEAG
                     EVEFYFDVLSPISGTITIRHVTLGDYVKEGSLLFEVMDLSHIWVVFDAYESDLPWIKL
                     RDKINFTIKSIPGRKFDATITFIDPVINRQTRVAGVRAELNNPRDILKPQMLASGILI
                     TMLPGSSDDELVIPKSAILWTGKKAIVYVMTDINNNLFQYREIELGAEAGNYYVVTNG
                     LSEGEKVATNGVFKIDAAAQLQGKQSMMNPEGGKSSTPHQHGEMKNDMEEEIKIPLRK
                     ETTGSAQGEKVIVDEKFKVQLTNFYKSQLQLQEALVATDAAKAKLGVPVAEAALSNVD
                     MTLVKGEMHNLWMSFHSKFREALDRIENSNEIETQRLIYADFSESLYGAIKKFGIVDE
                     TVYYKFCPMARENKGGYWLSSVEEIRNPYFGDAMLTCGANTEVIRQ"
     gene            complement(8538..9806)
                     /locus_tag="VGA21_12240"
     CDS             complement(8538..9806)
                     /locus_tag="VGA21_12240"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX9651324.1"
                     /translation="MKRSVKYIIVLTISLLVVNENVFGQDSIQAPVNDYLKIAAENNP
                     GLKSAFNQYLAALERMPQARTLPDPTIMFSLFISPVETRLGAQRAGISLSQAFPWFGQ
                     LKSQEMAAAQTAQAQFETFEEMRNELFFKVRSTYYDLYVLEATIAITGENIRLLQSFR
                     ELANVKLESAKGSAVDLLRVEIDLADLENQLKYLEDSRLPFQAEFKELLNTENTINIQ
                     LPDTLATLVLEKGKNESLDSINLHNPDLRKFDFQINALESEIDVARKMGLPSFSLGMS
                     YTIVTKRTDMDVPDNGKDMLTFPQVGVRIPIYRKKYQAMISERQFLKTSVSDQKENRI
                     NQLAVQLDKTWRDYLDAERRVGLYLNLTRLANQVLNILVTQYTSAGSDFEELLRMERQ
                     LLRYELELEKARADQNTAVAYIYYLTGKHF"
     gene            complement(9803..>10492)
                     /locus_tag="VGA21_12245"
     CDS             complement(9803..>10492)
                     /locus_tag="VGA21_12245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018480055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX9651325.1"
                     /translation="RFIKEKMDNGEFELPAGVSYKFTGNYENQIRAAKRLAVLVPISL
                     LIIFLILYFQFGNVQTTTMVFSGIFVAFSGGFIMIWFYSQDWFLNFAVAEISMRDLFD
                     IHTINLSVAVWVGFIALFGIATDDGVIMGTYIKQTFEREKPKNVKSVREAIIHAGVKR
                     VRPAMMTAATTIIALIPVLTSTGKGSDIMVPMAIPAFGGMLFQVITMFVVPTLYCMWQ
                     ESIVKNKTEQE"
BASE COUNT         2948 a         2343 c         1911 g         3290 t
ORIGIN      
        1 atcttgaaaa ctttcttgat acccggcaaa cccggtttga tacaattttt accggcacac
       61 cggataaccc acagttcaga gatatttacg cacccgtgga tgggtttgtt atcaatgctg
      121 ggttcaaatt aaaattctga atttgctgac attaaaacaa aggtgttgaa actattgacc
      181 gtaataagaa ttctttccgt gtttcctttc ataatgtttt tttattaaag catctgaaag
      241 ccgtggtgca tccgggttta ttgacaatgt aagaagcgcc attttggcca actcttccaa
      301 aactttggaa ttataaaccg cttttccgga atcttttccc caggtgaaag gtccatgatt
      361 gccaaccagc accatttcta cttctttata atccagtttt tttgaatcga agcaatccac
      421 aatttgtcga cccgtattat gttcataatc accttcaatc aaattgtctt tcatgggtgg
      481 agtgcacggg atatcatgag tgaggtgatc ggcgtgggtg gtgcccagaa tcggaatatc
      541 agcctgagcc tgcgcccatg cgacagaata agtggcatga gtatgacaaa ttccaccaat
      601 ttcaggccat gcattgtaaa gatacgagtg cgtttttgtg tctgaggaag gcttcaattt
      661 tccatctata atcttatttt caaaatttaa aatcactata tcctccggct taagatcagc
      721 gtatggtact ccacttggtt tgatggcaaa aactccatta tcccgatcag ccgcgcttac
      781 atttccaaag gtatagtgaa ccaatttcag cctgggaagc tgcaggtttg cttcataaca
      841 tttttctttc aattccttgt actttgacat tgcctcattt atgtttgact ttcaacaaat
      901 tcaccaagct ggcaatattt ctcgtataac ttcaaataaa tttccatttt ttccggatcg
      961 ggattatacg ttttctcaaa tccattcccc atggtcttga tggcttcatt cacatttttg
     1021 aaaataccag ccgcagttgc agcatacatg gccgcgccca gggcgggaac ttgatccgat
     1081 gaagctactt ttatcggcat atttaaaaca tttgccaaag tctgcatgac aaagggagat
     1141 tttttggcaa caccacccac tcctattacg gttcttatct caacaccttc atcactgaat
     1201 ctttctacaa ttttttttga tccgaaacaa atggattcta ccaacgcttt aaaaatttcc
     1261 ggggcttttg ttgccagatt aagattcata atggccgact tcaaagcttg gtttgcatcc
     1321 ggggttcttc ttccattaat ccagtcaagg gcaatcggca gcatttcatt gagtggaatt
     1381 ttttctgcct gagcggataa tccggtaatt atggaatctt ttaactttaa tttgtcttcc
     1441 gcctcaaggc gtagatttag attttctacg ggccaggaca gaacatctct gaaccacgcc
     1501 aggacatcac cgaaagcaga ttgccctgct tccagaccta ccatatcagg aataaccgat
     1561 ccattaacct gcccgcaaat tccttttaca gtttgattat ctacaacttc acctgggctt
     1621 acaataatat cacaagtaga agtacccatc acacgaacca gagcaaattc ctccacttcc
     1681 gcacccactg ctccggcatg tgcatcaaat gtaccaactg caattattgt tgccggagtt
     1741 aatcctaatt ttgaagccca ttcgtcagat aaattgccgg ctacgtggtc agatgtaaag
     1801 ctttgtgcat agagattacc tttaagcttt ttgaaagttg ggtgcagttg cattaaaaat
     1861 ttttcatcgg gtaacccgcc ccatgactcg tgccacatgg ctttgtggcc tgccgcacac
     1921 cggcttcgct taaaagattt aaggtcagaa gttcctgtca aaatgtacgt catcaggtca
     1981 caatgttcca cccaggaaaa agctgcctct cttatctttt catctttacg aataatgtga
     2041 aggatttttg cccagaacca ttccgaagaa taaatgccgc cttcatattt tgtataatcg
     2101 attcctcccc aggaacgggc caggcgatta atctcttcag cttctttatt tgccgtgtga
     2161 tctttccaca ataccatcat ggcattggga ttattttcaa attccggttt aagacaaaga
     2221 gccgttccac tttcatcaac cggaaccggt gaagatcctg ttgtgtcaat acaaattcct
     2281 tttacgaact caggttcaac gcccgaatct tttactaccc cggcaatagt ttcctcaata
     2341 ccttcaagat gatctaaaat gtgctgcctg aattgcgact ttgatgcatc acaatatttc
     2401 cctgccgccc accgcttgta ccaatgttcc tttgtcgaaa gtgtttcacc attaccggca
     2461 ttcaccagca ccgccctaac agagtcagta ccaaaatcca gccctattac atagtttatt
     2521 tccatctgcc tacgatgctg cgtctcttat cctctgccat ttaataaatt gcattcaaat
     2581 caaatgtaat catattttta gtgaatagga tgcagcaagt cttaacagga ggaatactta
     2641 cctcggcggg gaagcctttg ttatttagat attcgaaagg aaataattca caagtccgaa
     2701 cgctgcgcta atggcaatcc agggaatcat tcctacgcgg aattgataca tggcgataaa
     2761 tgaaatcgct atccagccta ttgaaataaa atcaatttgt aaaaaaataa attcgttggg
     2821 gaaaattacg gctttaccta aatacagagt aaggttcaac accaccccaa caactgccga
     2881 cgttactatg cttaaagctt ccctgatcat cctgttatct cttgtccttt caatgatcgg
     2941 cgcgccagcg aagataaaaa gaaagcatgg caggaaagta tagaaagcgg ttgttactaa
     3001 tccaaaacta cctgcaatca acgacccatt ttgattatta taggcagcca taaatccaac
     3061 gaacactaat accataacca gtggacctgg cgtagtttca cccaaggcaa ggccgtcaat
     3121 catttgcaac tcagatagcc aatgaagttt ttcgacactt acctgggcta cataaggcaa
     3181 gactgcgtaa gctccaccga atgttacaag tgccgcttgt gtaaaaaata aaacaagttg
     3241 tttccagaaa taaaagttaa tatcaaccag gtaaaaaatg atcaatggaa taaaccaaag
     3301 catagcaaca atagttagct gccggactaa cagccataca tggggcttaa cttcagattt
     3361 gtgttcatta ttgataaaat aaccggattc atctttaatt gattggttta ttgctgtctg
     3421 agatttcacg aaaagatttg gatagaactt ttgaactgca aatgcaatta acagagcagc
     3481 gaggatgata attggaaagg aaatgttaaa gaagaaaata caaatgaatg ctccaagacc
     3541 aaccagatag tgaaaaaagg tatgcagaga ctttttccca atttttatta acgcgagaat
     3601 tacaattgcc aaaaccgcgg gttttaatcc ttcaaacaag gcgtaaaccc aggggatgtc
     3661 accatatttt gcatacaata cagttaatgc cagcagaatg aacatggagg gcaaaacaaa
     3721 aaaaattccg gcaattagcc caccttttgt cccatgcaac agccaaccgg tataagttgc
     3781 cagttgttgg gcttccggtc ccggtaatat cattgtataa tttaatgcat gaaggaaacg
     3841 gctgtttgaa atccatctct ttttttcaac cagaaattca tgcataattg caatttgacc
     3901 ggccgggccg ccaaaactga taaatcccag cttcagccaa aaccaaaatg cttctttaaa
     3961 ggaaggtttt tgcatttatc tattacatac acgaaaatca cttgctgaaa tcaaaagaga
     4021 tcccttttct taaggataac gccttttcat atactattac agctgatgca acatcttgta
     4081 acgccatacc cgtactatcg aatacaaata tttcatccgg cgattttctt ccttgttttg
     4141 taccggccac tatttctcct agctcggcat aaacaaagtc ttcatcaacg agacctgcct
     4201 caattgcatg gtgcagctct cctattttgg aagcctgagc agttaaatcc gttacaagtt
     4261 tcgatttttt caaaacctcc ggttcgagct cctgcttttc ttcgttgtcg gctccaaccg
     4321 cggcaaggaa taaaccaggg taaatatacg cctcctttac aaaagctttt tcagatgtcg
     4381 tgcaagtgac acaaatgtcg ctcagtggaa taaccttctc aatgctgtca gttaattcta
     4441 catctataga aagagtgtcc ctgacgtcct tggcaaaaat ttccgcggtt tggtggttgg
     4501 tatcgtaagc ataaaccttt tgaatatctc taatttctct caacatccta atcgatattt
     4561 ttccctgctc gccgcatccg cagattgtta caatttttga atcaggcttt gaaagatatt
     4621 ttgccgcgac tgccgttgcc gcaccggttc ggattatggt caattcaata gagtccatga
     4681 tggacagagg tcggccattt tttgcatcca ttaccaggac aactccctgg atggtgggca
     4741 gattaaaatt ttttctattt tcagggaaat tggcatttgt ctttgccaca aagtagtggt
     4801 tcgcgagatt catcaagccg gccttaatat gaaaccctcc ggcatctgca tgcatgccaa
     4861 gaattcccgg gggtgtcgct ttgcccatac catacagctt aaatgcattt tcaacggcac
     4921 tgatcccttc cccgattgtc agcaattcct tcacgtttgt tctggacaat aagattagaa
     4981 ggttatcatt catagaattt aaaaattatt gttcttgact tacaggatgc aaactaaagt
     5041 gattgaagaa agaaaatata gaagggaatt aacctttggg attctattta cgccgggatt
     5101 ctcttaattt atttttttta taagccattg gtggaatacc aaggtgtttc ttaaatattc
     5161 tggtaaagtg gctctgatcg gaaaacccgc atagaaatgc aatttcggaa agagaatatt
     5221 ttgttgtctc aagcagtttc agtgcttttt caattctaag tttcctcaga taatcgccaa
     5281 aatttaaatc ctcaaagtat ttggaaaatt ctctggacaa ataagcggga tgaacctgaa
     5341 gattctctga aacttctttt agacttaaac ttaactcggt atcaatttga tcctgaatga
     5401 tttccttcaa ctcctctacc cattttggaa tctttttttc tgagttttta tgcttcagga
     5461 attttcggta tacgtcaacc aatagtatct cggttggatt ctgagtgtgc ttcaaatctt
     5521 tcaaatgctt cgcccaggag tataaggcat cataaataat aaagcctgct tccaataatt
     5581 catgatcttt ttttatgttg aaggcaaggc cggacgagat tgcccaaagt cctgaagcct
     5641 ggcttgcaag gctgtgccgg tcagtatcag ctcctctcac tattttggca ataattgcca
     5701 ctgccggatc ttcaatttta tgtttgttta taaagtaatc aaaagtgcat tgatcgtcgt
     5761 aatgggtata ttccacacca ggaatgtcat acggtatagc gttgagaatt ttggcgcttg
     5821 attttacatc cttaaatgga acgaaatgaa aaacagcatc gtgatcaatg aattttttaa
     5881 tcagccaggg gcaggcaata cgatcaattt tgggactttc acgcgttatc cattgcatgt
     5941 cttaagaaac ggaatcgatt ggctttctgt tattatcaat acctcttgac ttcttcatct
     6001 ctgaaggagt aaaacccgta attttcttga attgagctga taaatgtgaa acactgctat
     6061 atcccagccg gtcagctatt tcactcaaat taaattcatc ataaaacaag tattccttaa
     6121 ccttttcgac cttttgccga attatatact gctcaagggt tataccttga acacttgaaa
     6181 acaggctgct taagtaattg tactcatatt tcagttcctc tgaaagcagg tttgaccagt
     6241 taactttcaa ttcgacatta tctgagtggt ggatcatttg gataatttta ttcttgatgg
     6301 cttcaattat ccttgccttc ttgtcatcaa tgcgttcaaa tcccaaattc cttaacgcag
     6361 catccaggtt atctttggtg actccggata tatcatcttc ctgaattgtt acttctccaa
     6421 gtgaaatttt ttgagggtgc aaaccttgct tttcaatttc ctgttttacc accatgatac
     6481 agcggttgca aaccatattc ttaatataaa gggtagtcat tattaatgcc tttactaact
     6541 cacaatttac ctattatttt gaaaatcaga gcagcactat gcttaaatca gacaattacg
     6601 ttaaggggtg caaaatcgcc aattattgca atagattatt attacaggaa tcttaatttg
     6661 tgtgaaagaa aatttattta ccgcttccta cagattctta ttgtctgatt acttccgtat
     6721 tcgctccgca tgtcagcatt gcatcaccga agtaagggtt tctgatctct tccacagaac
     6781 ttaaccaata accaccttta ttttcccggg ccattggaca gaacttgtag tataccgttt
     6841 catcaactat cccaaatttt ttgatcgcgc cgtatagcga ttcactgaaa tctgcatata
     6901 ttaatcgctg ggtttcaatc tcatttgaat tttcaatccg atccaatgct tccctgaact
     6961 ttgagtggaa cgacatccac agattatgca tttcgccctt taccagggtc atatctacat
     7021 tggaaagagc cgcttcggcc accggcactc ctaattttgc ctttgcggca tccgtagcca
     7081 ccagtgcttc ctgcagttgt aactgcgatt tatagaaatt ggttaattgt actttgaatt
     7141 tttcatctac aattaccttt tcgccctgcg cacttcccgt ggtttccttt ctgaggggaa
     7201 ttttaatttc ttcctccata tcattcttca tctcgccatg ctggtgaggg gtagaacttt
     7261 tacctccttc cggattcatc atgctttgct tgccttgcag ttgtgccgct gcatcgattt
     7321 taaacacgcc attggttgca accttttcgc cttcacttaa tccatttgtt accacgtaat
     7381 agttacctgc ctctgcaccc aactcaatct cccggtattg aaaaagatta ttattaatat
     7441 cggtcataac atacactatt gcttttttgc ctgtccacag gattgcagat ttgggtataa
     7501 ccaactcatc atcactgctt cccggaagca tggttatcag gatgccagag gccaacattt
     7561 gcggtttgag gatatctctg ggattattaa gttctgccct gactccggct accctggtct
     7621 gacgatttat gacagggtca ataaatgtaa ttgtagcgtc aaatttacgt ccgggaattg
     7681 atttaattgt gaagttgatt ttatcccgga gtttgatcca tggaaggtca ctttcatacg
     7741 catcgaatac aacccaaata tgactcaaat ccataacttc aaacagcagc gagccttctt
     7801 taacataatc tcccagggtt acatgacgaa tagtaattgt acccgaaatg ggtgaaagta
     7861 catcgaaata gaattctact tctcccgcct cttcaatgtt attgatttgc tgttcagtta
     7921 aactccataa tttaagcttc tgcctggacg cttcgtaata tctgggatta ctttctttgt
     7981 atttcacagc ttcaaaaagt tccttttgtg cagttacgag ttcaggtgaa tatatttttg
     8041 caaggacctg cccttccctt actttctggc cggtaaaatt gacggataat ttctcaattc
     8101 tgcccgcgaa acgggaagta agctgagaaa tacgccgttc atccggcatc actttgcccg
     8161 gtaaataaat ctcgttataa ggcgctttcc tttctattac cgttgttgag acttcagcaa
     8221 tttttatcgc tgcattagaa agttttacgg taaactgact gacttcctca tccaaatcag
     8281 tctcatccat ttcaacaggt acaagatcca ttccgcatat tggacatttt ccgggaccgt
     8341 cttcccttat ttgcggatgc atggagcaag tccaaacctg gccggcttca tgatcttcaa
     8401 gagtatggtc cgctgcaaga ttttcagaag gtttttgatt tcccccaaaa atcagccacc
     8461 caattaaaat tccggcaatc agaattacca aattagtgta ggtgacttta cttttaattt
     8521 tatcaagtat tttcatttta aaaatgtttt ccggttaaat aatagatata agcgacggcg
     8581 gtgttttgat cggccctggc tttttcaagt tccagttcat acctcaaaag ctgcctttcc
     8641 attcggagca attcttcaaa atcacttccc gcggatgtat attgagtgac caggatattc
     8701 aacacctggt tggctaaacg ggtcagattt aaatacaagc caactcttct ttcagcatcc
     8761 aggtaatctc tccatgtttt atccaattgt accgccagct gattgattct attttccttt
     8821 tgatccgaaa ctgaggtttt cagaaattgc ctttcactaa tcattgcctg gtatttttta
     8881 cggtaaatgg gtattcttac tccaacctga ggaaatgtta gcatgtcctt accgttgtca
     8941 ggaacatcca tatctgttct cttggtcacg atggtataag acatccccag gctgaatgat
     9001 ggcaaaccca tttttcttgc cacgtcaatt tcgctttcca gggcattgat ttgaaaatca
     9061 aatttcctga gatcgggatt atgcaggttt atggaatcaa gagattcatt tttacctttt
     9121 tcaagcacaa gcgtggccag agtatcgggc aattgaatgt taattgtatt ttccgtatta
     9181 agcagttctt tgaattccgc ttggaacggt aatcttgagt cttccagata tttcagttga
     9241 ttctccaggt cagcaaggtc tatttctact ctcaaaagat cgacagcact gccttttgca
     9301 gattccagct ttacattggc caattcacgg aaggattgca gcaatcgtat attttctcct
     9361 gtaatcgcga ttgtagcctc aagaacatat agatcataat atgttgaacg cactttgaag
     9421 aataattcgt tccgcatttc ttcaaaagtt tcaaactgcg cctgggcggt ttgggctgca
     9481 gccatttcct gggactttag ctggccaaac cagggaaaag cctgacttaa ggaaataccc
     9541 gcccgctgtg cccccaatct tgtttccacc ggacttataa ataggctaaa cataatggtt
     9601 gggtcaggca atgtcctggc ctggggcatt cgttcgagtg cggccaggta ttgattaaac
     9661 gcactcttca gtccaggatt attttcagca gctatcttca ggtaatcatt tactggagcc
     9721 tggatactat cctgtccgaa tacattttca tttaccacca acagggaaat ggtaagcact
     9781 attatatatt ttacacttcg tttcattctt gttcagtttt gttcttaaca atactttcct
     9841 gccacatgca gtaaagtgtg ggaaccacaa acattgtaat tacctggaaa agcattccgc
     9901 caaatgcagg aattgccatt ggaaccataa tatcagatcc ttttcctgta gatgtgagca
     9961 cgggaatcaa agcaataatt gtagtcgccg cggtcatcat tgccggtctt actcttttca
    10021 caccggcatg tattatggct tctctcacag attttacatt tttgggtttt tcgcgctcaa
    10081 aggtttgttt gatgtacgtg cccataatta caccatcatc ggtagctatt ccgaaaagag
    10141 caataaagcc tacccacact gccacactca ggttaatggt gtgtatatca aatagatccc
    10201 gcatgctaat ttccgctact gcaaaattca gaaaccaatc ctggctataa aaccaaatca
    10261 tgataaagcc tcctgaaaac gccacaaaga tccctgaaaa caccattgtt gtcgtctgaa
    10321 catttccgaa ttgaaaataa aggatcagaa aaattatcag caacgaaatg ggcactaaaa
    10381 ctgcaagtct tttagcagcc ctaatctgat tttcataatt acctgtgaat ttataagaga
    10441 ctccggcggg aagttcaaat tcaccattat ccattttttc tttaataaac ct
//
LOCUS       DASWHX010000187         4559 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_943939, whole genome shotgun sequence.
ACCESSION   DASWHX010000187 DASWHX010000000
VERSION     DASWHX010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 4559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4559
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_943939"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..203
                     /locus_tag="VGA21_12250"
     CDS             <1..203
                     /locus_tag="VGA21_12250"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEX9651326.1"
                     /translation="STILKTETEVGIRIINSLGRTVFREEGLPGKSGENFFVINPILD
                     PGGYLLTISAGNFNEVFRIVFK"
     gene            305..1492
                     /locus_tag="VGA21_12255"
     CDS             305..1492
                     /locus_tag="VGA21_12255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651327.1"
                     /translation="MKEMALKDNWNRYHVPIIIILGLLINFFLYQKFGVQIVSDSHRY
                     IALPPIPSMEFGIDKNFWYVGYTFFVLAADCLLTSQLDVIIFQVIIYCISLIFIYFTS
                     FKLFENTNTAFLTTLTYLAFLDITQWNFYILAESFYVSFVVIITYFILSFNRNVKSLF
                     IIVPLILYCFFIKPTGFSILVAFATLVVSVFWEELTKKRLAFSIICLLIMTSGFFLLN
                     KMLATFIESIIYNDYQYGELIYGIHHYPDIPTYNLLIIREDDLTFPSAEAAPLFRIAE
                     FAIRNPFYFIKLFSAKAFYLLFQVRPYYSLPHNVYLLGILIPVYAGFIISVSKSRLPG
                     RVILFALVFILTHTGIVCLTTVDWDGRFLLPMLPLIFIIGVPSLIELVKKIFHKETAL
                     KRL"
     gene            1510..2061
                     /locus_tag="VGA21_12260"
     CDS             1510..2061
                     /locus_tag="VGA21_12260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011583893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HEX9651328.1"
                     /translation="MNLGLTLLPLSPYLLEGMESSLILSENQISQKILRMAYEIYENN
                     LKEKEIIIAGIEGQGTVLAKSLAKAVEKISPVKVKLVKISIDKEKPESSEITIDQEPK
                     ILKKKVIILVDDVLNTGRTFAYGMRPFLSFDVKKIEVAVLVDRGHTLFPVSAKYRGYE
                     LSTTLNETIIVNLESQKKGVYLE"
     gene            2063..2881
                     /gene="panB"
                     /locus_tag="VGA21_12265"
     CDS             2063..2881
                     /gene="panB"
                     /locus_tag="VGA21_12265"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019596756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEX9651329.1"
                     /translation="MSVPKSNIKKVTTHQLQEMTNRGEKISMLTAYDFSMARILDGAG
                     IDVLLVGDSASNVMAGHETTLPITLDQMIYHASGVVRGVSRALVIVDIPFGYYQGDSQ
                     EALKSAIRIMKESGAHAVKIEGGSEIKESVERILSAGVPVMGHLGLTPQSIFKFGTYT
                     VRAKEEAEATKLLEDSKLLEECGCFALVLEKIPAKLAKKVADQLTIPTIGIGAGADVD
                     GQVLVMQDMLGITKDFKPRFLRRYADLNKIISDAVSQYISDIKAGDFPNDEEKY"
     gene            complement(2878..3447)
                     /locus_tag="VGA21_12270"
     CDS             complement(2878..3447)
                     /locus_tag="VGA21_12270"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEX9651330.1"
                     /translation="MISLILPLLLSVFAVNVLGENDVEEICLNDKEKELYQLIMEYRK
                     EKGLNSISLSPSLSKVAQTHVNDLNENYKLDEKCNAHSWSRKGKWTSCCYTEDHKKAE
                     CMWDKPKELAEYNSKGYEIVSWGFPELSALTALKEWQKSPGHNPLLINSGVWEQARWQ
                     AIGIGLRGNYAAVWFGVMKDTNSEIQFCD"
     gene            3546..>4559
                     /gene="icd"
                     /locus_tag="VGA21_12275"
     CDS             3546..>4559
                     /gene="icd"
                     /locus_tag="VGA21_12275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014796260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HEX9651331.1"
                     /translation="MTSKKITITDGKLSVPDNPVIPFIEGDGTGIDIWPASRLVFDAA
                     VENAYGGKRKIEWMEVLAGEKAFNETGNWMPEETLNKFREYLVGIKGPLTTPVGGGIR
                     SLNVALRQELDLYACVRPVRWFHGVPSPVKDPSTTNMVIFRENTEDIYAGIEFMHGTP
                     EADKIIKFLTGEMGIKKIRFPETSSIGIKPVSLEGTERLVRSAIEYALSEGRPTLTLV
                     HKGNIMKFTEGAFKKWGYDLAEREFGDKTFTWAEYDRIAVAEGTEAANSAQQKAIEAG
                     KLLIKDVIADAFLQQILTRPAEYSVIATLNLNGDYISDALAAIVGGIGIAPGANINYI
                     TGHA"
BASE COUNT         1381 a          875 c          929 g         1374 t
ORIGIN      
        1 tctccacaat actgaaaacg gaaacggaag ttgggattag aattattaac tccctgggca
       61 gaactgtatt tagagaggaa ggattacctg gaaaatccgg ggagaatttt ttcgtgataa
      121 atccaatttt ggatccggga ggttatttat tgacgatctc tgctggcaat tttaatgaag
      181 tatttagaat agtttttaag tgaaaatcaa agaatatatc aacgttggtt tcacactcaa
      241 tttgatgaat tttgaagccg attctccaaa gagattccgt ataaatttcg tattctgaat
      301 taggatgaaa gaaatggcgc tgaaagataa ttggaataga tatcatgttc ccataataat
      361 aattttaggg ctactgatca atttcttcct ttaccaaaaa tttggagtcc agatcgtctc
      421 tgattctcac cgctatatag ctttgccacc tatcccttct atggaattcg gaatagataa
      481 aaacttctgg tatgttggtt acacattttt tgttttggca gccgattgtc tactaacctc
      541 acaacttgat gttatcattt ttcaggtcat tatttattgt ataagcctga tatttattta
      601 tttcacctct tttaaactgt tcgaaaacac caatacggct ttccttacta cacttactta
      661 tcttgccttc ctcgacataa ctcaatggaa tttctatatt ctggcggaat cattttatgt
      721 gagttttgtg gtgatcataa catactttat attatcattt aaccgtaatg tgaaaagcct
      781 gtttatcata gtgccactaa ttttatattg ttttttcatc aaacccaccg gcttttcaat
      841 actagtagct tttgcaactt tagtagtttc agtattttgg gaagaactca caaaaaaaag
      901 attggcattt tcaattattt gtcttttaat aatgacctca ggattctttt tactaaataa
      961 aatgctagca acatttattg aatctataat atataatgac taccaatatg gagaattgat
     1021 ctatgggata catcattatc cggatatccc aacttataat cttctaatta tcagggagga
     1081 tgatttaaca tttccatctg ccgaggctgc cccccttttc aggattgctg aatttgcaat
     1141 tcgaaaccca ttctatttta ttaaactttt ctccgctaaa gcattttatt tgcttttcca
     1201 ggtaagaccg tactatagcc tgccgcataa tgtatacctg cttggtattc ttattcctgt
     1261 ttacgcggga ttcataataa gtgtcagcaa atcgcgctta ccagggagag taatcttatt
     1321 tgcgttggtt ttcattttaa cacatacagg cattgtttgt cttacaactg ttgactggga
     1381 tggtagattt ctacttccaa tgctgccgct aatatttatt ataggagttc cgtccctgat
     1441 tgaattagtt aaaaaaatct ttcataagga aacagccctc aaaaggctat aatcgcccat
     1501 ccgggtgcta tgaatttagg gctaacccta ttacctttat ctccttatct tcttgaaggc
     1561 atggaatcga gcttaattct ttctgaaaat caaataagtc agaaaattct ccggatggcg
     1621 tatgagatct atgaaaataa tctcaaagag aaagagatca taattgcggg tattgaagga
     1681 cagggaactg tccttgccaa atcacttgca aaggcagtag agaaaatttc acctgttaaa
     1741 gtaaaattgg tgaaaatatc gatagacaag gaaaagccgg agtcttcaga aatcaccatc
     1801 gaccaggagc caaaaattct gaaaaagaaa gttattatcc tggtagatga tgtgctgaac
     1861 accggcagaa cttttgctta cggcatgcgt ccatttcttt ctttcgatgt aaagaaaatt
     1921 gaagtggcag ttttggtgga tcgcggccat actttatttc ctgtttctgc aaaataccga
     1981 ggttatgagt tgtcaacaac ccttaatgaa accataatag ttaatcttga atcacaaaag
     2041 aagggcgttt acctggaata aaatgtcggt accaaaatca aacataaaaa aagttaccac
     2101 tcatcagttg caggaaatga caaaccgtgg cgaaaaaatt tccatgctaa ccgcttatga
     2161 tttctccatg gctcgtattc ttgacggagc cggtatcgat gtgcttcttg taggtgactc
     2221 tgcttctaat gtaatggcag ggcatgaaac aactcttcca atcactctcg accaaatgat
     2281 ctaccatgcc tcaggagttg tacggggcgt ttcacgggca ctggtaatcg tagatatacc
     2341 ctttggctat tatcagggag attcgcagga agcgttgaag tctgcgattc gaatcatgaa
     2401 agaatcggga gctcatgcgg taaaaattga aggcggaagc gaaataaaag aatcggtgga
     2461 gagaatttta agtgccggag ttccggttat ggggcacctt ggtttaaccc cacagtctat
     2521 ttttaaattc ggaacatata ctgtacgagc caaagaagag gctgaagcaa ccaaactgtt
     2581 ggaagactct aaattattgg aagagtgtgg atgctttgca ctggtacttg aaaaaattcc
     2641 tgcaaaacta gcgaaaaaag ttgccgatca gttaacaatt ccaaccatcg ggattggagc
     2701 cggagcggat gtggatggcc aggttctggt aatgcaggat atgctgggta tcacaaaaga
     2761 ttttaagccc cgtttcttga gaagatacgc agacctgaac aaaataattt ccgatgcggt
     2821 gtctcaatat atttctgata ttaaggcagg cgatttccct aatgacgaag agaaatacta
     2881 atcgcagaat tggatctcag aattggtgtc tttcatcact ccaaaccaga ctgcggcata
     2941 gtttccacgc aatccaatcc ctatagcttg ccacctggct tgctcccaaa ctccagaatt
     3001 tatcaataat ggattgtgcc ccggactttt ttgccactcc ttcaaagcgg tcagcgctga
     3061 taattcagga aacccccagc ttacaatttc atatcctttg ctgttgtatt ctgcaagttc
     3121 ttttggttta tcccacatgc attccgcttt tttatgatct tcggtataac agcaagatgt
     3181 ccacttgcct ttcctggacc agctgtgagc attgcatttt tcatccagtt tataattctc
     3241 attcaggtca ttgacatggg tttgtgccac tttgcttaaa gaaggagaca atgaaatgga
     3301 attcaatccc ttttctttgc ggtactccat tattaattga tacagctcct tctccttgtc
     3361 attaaggcat atttcttcga catcattctc acctaaaaca ttgacggcaa aaactgataa
     3421 aagcaagggt aaaatcaatg aaatcataac tggccggtta atttaagcaa aacttgtggc
     3481 gtgtgtcatt taaaaactgt cgggattgtt taaattcacc acttattaaa taaaaatagt
     3541 aaataatgac cagtaagaaa ataactatca ccgatggaaa gcttagcgta ccggataatc
     3601 cggtaattcc tttcattgaa ggagatggaa ccggtattga tatttggccc gcttccaggc
     3661 tagtatttga tgccgcagta gaaaatgcat atggtggaaa acgaaagatt gagtggatgg
     3721 aagtgcttgc cggggaaaaa gctttcaatg aaacaggaaa ctggatgcct gaggaaactc
     3781 tgaataaatt tcgggaatat ctggtcggca ttaaaggtcc cctgaccacc cccgttggag
     3841 gtggcattcg gtctctgaat gtggcccttc gtcaggaatt ggatttgtat gcatgcgtga
     3901 gaccggtgag atggtttcat ggtgtgccat cacctgtaaa ggatcccagc actactaaca
     3961 tggttatttt ccgtgaaaac actgaagata tttatgcggg gatcgaattc atgcatggaa
     4021 ctccggaagc tgataaaata ataaagtttt tgacaggaga aatgggcatc aaaaagatca
     4081 ggtttcctga aacctcatcc atcggtataa agcctgtttc gcttgaaggt acagaacgcc
     4141 tggtaaggtc agcgattgaa tacgcgctta gcgaaggacg acccacctta acgctggttc
     4201 ataaaggtaa tattatgaaa ttcaccgagg gcgcattcaa aaaatgggga tatgatctgg
     4261 cagaaagaga atttggagat aagactttta cctgggcaga gtatgacaga atcgcggttg
     4321 cagaaggaac tgaagctgca aattccgctc aacaaaaagc aatcgaagcc gggaaactat
     4381 taattaaaga tgtaattgct gatgcatttc ttcaacaaat cctgacgcga ccggccgaat
     4441 actctgttat tgcaactctg aatctgaatg gagattatat ttctgatgcg ctggcagcaa
     4501 tcgtgggcgg aataggaatt gctccgggag ccaatataaa ctatatcaca ggtcatgca
//
LOCUS       DASWHX010000188        41275 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_2070839, whole genome shotgun sequence.
ACCESSION   DASWHX010000188 DASWHX010000000
VERSION     DASWHX010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 41275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41275
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2070839"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..417
                     /locus_tag="VGA21_12280"
     CDS             <1..417
                     /locus_tag="VGA21_12280"
                     /inference="COORDINATES: protein motif:HMM:NF039201.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEX9651332.1"
                     /translation="FRSFQANKEEGQSERSWDSKKPKSISRLIPSKLACGSNLSAYKF
                     VEIGSGGPIHFRKDEIEAQFFPNPAKDYITIKHNISPLKKPYVKIYDSQGGELVVDKA
                     LSYEENQINISHLACGNYVIHINFNGGVFISQLIKE"
     gene            414..941
                     /locus_tag="VGA21_12285"
     CDS             414..941
                     /locus_tag="VGA21_12285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651333.1"
                     /translation="MKKAIVLFIGLALVNSCNPFTGKPENIYAYFVFSQSEGDFFNSE
                     PLYDETELRVANPNIPGKHPFIEVKERDGFHQFGLSGWTVPITYFDYGNGDVDTLAVS
                     LDPDFDLPMSNFNRLKSVKFYLNNILIEEWDFKNHPEKRDSILMFNTEEARLNGFNSE
                     VVIRVPKSPDLDELN"
     gene            complement(1022..1795)
                     /locus_tag="VGA21_12290"
     CDS             complement(1022..1795)
                     /locus_tag="VGA21_12290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007653842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX9651334.1"
                     /translation="MEFVKYSVEQRIATVILSRPAKRNALNNQFVRELDETFAKIDKD
                     KRTKVVIIKAEGDVFCAGADLEYLKQLQQNTFEENLEDSTQLKNLFYRIYQSDKIVIA
                     QMHGHAIAGGAGIITLCDYVFASNQAKIGYTEVKIGFIPAIVTLFLIKKAGEGRAKQL
                     LLSGDLIDAETAVNFGLVYKVVEANELESSVLNFAKKLVSDNSGESMKMTKQLIHKVQ
                     KMNEEEALDYAAKLNAKARESKDCRKGIEAFLDKKNIEW"
     gene            complement(1841..3424)
                     /locus_tag="VGA21_12295"
     CDS             complement(1841..3424)
                     /locus_tag="VGA21_12295"
                     /inference="COORDINATES: protein motif:HMM:NF015714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HEX9651335.1"
                     /translation="MQELAPMPFARLTLVFSLGILSCRYAAIPHDFSYFILLSAYLGV
                     AISFRKKLFSNSHLVGILGLLTIYLGGWSIYSNQLQTENPHLNSPKLLESESFLLWLN
                     KPLDKKGERNRGIAEIIGFQIDGNWRKCNHRILVTLTEDVDGKLGDLLLVKSHPDTIK
                     NFNDNLEFDFKAYYALQGIYLTQYITPKKVVAKSVRDIPLHQSLAFNTRIQIEQTIDK
                     YFGDSQSGGVLKALTIGQKSDLDPDVKENFSNSGTMHVLAVSGLHVGIFYGIFLFIFN
                     KLINTRWKYLIITFIVIQLWFYALITGLSPSVVRAVTMFSILALGKLIDRRSNAFNLV
                     FASAFMILLFEPGLLFSVGFQLSYLAVLSILFFGPKLTRAQPVWIPPKLWTLLAISLA
                     AQIGTLPLSLYYFKQFPTYFLLSNLLVIPAVFIILNGAILFIAIGFIDFFQQGLAYLL
                     DKFVNMMLLSVELLNKLPLNVISTRVFGTKEVILLYLLICSLSIFWLKRKYSWLIYSC
                     ALIGVFFISRIFTLWMYYH"
     gene            complement(3449..4405)
                     /locus_tag="VGA21_12300"
     CDS             complement(3449..4405)
                     /locus_tag="VGA21_12300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013453508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEX9651336.1"
                     /translation="MVEKVITLENISLIDFLGIEDQNIKEVASAFPKSKIISRGNEIR
                     IKGNSAEILRINAILNSLLEHYHKYGKITHTNIQDVLTSNINEEKEIPSKDEVLIYGP
                     SGSVIKPRTINQQKLVYSVRENDVIFVVGPAGTGKTFVSVAMAVQALKNKEVKKIIIT
                     RPAVEAGENLGYLPGDLKEKIDPYLRPIYDSLDEMVPFEKLKFYLESRVIEIAPLAYM
                     RGRTLNNAFILLDEAQNTTPMQIKMFLTRMGPSSKVIVTGDMTQIDLPKNQNSGLAEA
                     IGILKDIPGVGFIELQPKDVVRHKIVRDIVEAYQKTIIKKDN"
     gene            4498..5262
                     /locus_tag="VGA21_12305"
     CDS             4498..5262
                     /locus_tag="VGA21_12305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009577835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HEX9651337.1"
                     /translation="MAIITFTSDFGETDHYIAAVKARILAFNPNLTIVDITHNVQAGD
                     LAHASFVVKSVYRDFPKGTTHLIAVGSAKSRFVGLKVNEHYFLGHDNGLFGLITEETS
                     TEAIDLNNPHKIRSQFPAKDILATAAAKLASGVGFSDLGVRIDDFKIMIPRRVKSTKS
                     QIAGHVIRVDYYGNLISNIEEETFRFLTRDKRFAISFGRERAFHVHDYYDSVENGECF
                     IIFNSLGLLEIGINKGNAAQLLGLDYDSPIIINIEQ"
     gene            5262..5555
                     /locus_tag="VGA21_12310"
     CDS             5262..5555
                     /locus_tag="VGA21_12310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014797667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEX9651338.1"
                     /translation="MLTRIVIMNFEEENIQSFLKIFEQAENKIRTLPGCIHLELKRNV
                     HNHSQLATISKWNSEKELNNYRDSEFFKDTWQKTKALFSTDAEAFSFVDVDLK"
     gene            5598..5679
                     /locus_tag="VGA21_12315"
     tRNA            5598..5679
                     /locus_tag="VGA21_12315"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5632..5634,aa:Leu,seq:caa)
     gene            complement(5707..8811)
                     /locus_tag="VGA21_12320"
     CDS             complement(5707..8811)
                     /locus_tag="VGA21_12320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651339.1"
                     /translation="MKTRIIKQTFISFGLLLAISFYAISQKITIADGNFNDPAIWSPA
                     GVPASSDDVIINRDVIINTAHIINSLLINNKENYTATVEMYAGAGLSVVRDVQLEMAP
                     GILKEVEIIMADGALLDIGGNMNFIRENEGQDIGCRLFMTGGTTSVAGDLNYNYLTGG
                     WTDGTIFFPEFITEIQLFGNSELSVSGTMNLIQDEATTLSTFQYILQVRTLDNAKLNV
                     GSLVMQQDVDVSAYTPQIHVESLGASTITIAGDLTMNQMGAASNLLRFESEDISSFSI
                     LGNIIINKQLGRTILFELWNNSTGTVNGDISVTKSGGTSNIAIRARDQSNLIVNGPNG
                     LTKTDSGGGSLFNLDVEANASMNFTNGSVAIYADNTTAGSTILEINGELSIGKDLIIN
                     ALYRRFNINLTGTLLNPALLSVGGNFNLTFNPSNLSPSGVAFTNAKLIVGNDVNIYQN
                     ASSDSKELFLTFDGDIDGTIGGNFTWEDKRGISNFTLGGIVAGSAGTLQMGNLNLKHT
                     AILSHSSSAMTTTLKQDAAIVVSGNSQLTIDTDFGLTTERNKLYLLDNSNLNVMGDFT
                     ISSKNQQNATLFLFNNSSLTLGGELLRPLNFGDISFNDQSYMTFNGNSPQVIPAQSLT
                     GAGTDGIYFNRVVANNTSPTIPQLTLISDVNLNQLDFMSGIIKTSSTAKIIFREGGIS
                     TGSGNQRFIDGPVQKNGTTDFEFPLGDSNGTIDRWSPLSVSNITGGASTLTTTAEYFI
                     GSVPNSDDIDPTLATISGIEYWDVSPSEGISAEVTLFWKDADFSEITDEEDLTFAHYN
                     STSSRWEDMFGAVGEGSVVGPGGGEGSITAYLSSFSPVSFGSKGGVNPLPVILTDFKA
                     ELKNKFAEISWITSSEIENDRFEIEKSMNGREFLKISEVKGHGSTSEENFYIIKDRPL
                     NGVNYYRLKQIDFSGKYTYSKVVSVEKNDLLASTPVIYPNPVSSGIIGKITIDMNPEI
                     AINGDPLSITLIGPTGNTIIRKEVVYSGNSINLDLDQDLNKGIHFVIVSNVLKRYQTK
                     LLVE"
     gene            8949..9638
                     /locus_tag="VGA21_12325"
     CDS             8949..9638
                     /locus_tag="VGA21_12325"
                     /inference="COORDINATES: protein motif:HMM:NF024957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEX9651340.1"
                     /translation="MKYVKHHFNNALKHFILSAIFLAFSGNDVISQYKSPAGSLNFGI
                     STYQGISFLEGAQFTDIKPGYAQASGLNIKYYQTITIGLQVDILYSKAAGTFSKIEAL
                     SNQNNLKIKTKQKLNFISVPISINLYLSDRFESENIHIKLGVVPSFLIKAVKSSNNPE
                     ENGDKVENSNDFRSMDFGFLAGIGVEVRRFNLIGEYYLGVYNLNANKNGSRITNHGPR
                     IGLNYFLKRWD"
     gene            complement(9614..10681)
                     /locus_tag="VGA21_12330"
     CDS             complement(9614..10681)
                     /locus_tag="VGA21_12330"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX9651341.1"
                     /translation="MRQSKISLDHDNTYFQDIVERGFEMVIKIDFNYSFKYLNKPARH
                     FFRNYELENEGLKRVLSTKFPKIRLNEAIEQGCITGETHLENNENVSVPLSYLLLSHS
                     NEYEGEMFTLILRDNTSKKAIESSFSQKRDELNRFIYRVSHDLRGPIASMMGLSNLVH
                     REINEAKSLDYFSLFQSQVERLNNIVIALGKHSVLEFSDYEQEEFTFNNIVADMLASY
                     QDLNSKVVKINKSIIKNTFIGNQFALQTILHSLIDNSCNFLSEHRPGLISIEVYDNTE
                     FIFITIEDNGIGIENIYKQNIFQMFYKASTKSRGSGLGLYLFDTAVKKMGGSYLMESE
                     VDKGTRFEVIIPIDQSHLFRK"
     gene            complement(10691..11470)
                     /locus_tag="VGA21_12335"
     CDS             complement(10691..11470)
                     /locus_tag="VGA21_12335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651342.1"
                     /translation="MTKTILALGKSGPVMNAIVNELLSAGNHVSIVGCPERNAISQPS
                     LVKTVGICSVRDLISNGSFETIFINLYGEPSTAENRLIQQAIKCAANKKNITFLILRR
                     AGRNNSIELSNPDNQHSCKFAVIISSFIFEDLLLSSFKTNIHLSRELLAGSYGISASD
                     IGRQIDKAIKMKNQFNGITYFVQGPEKIDLNEVTAKTRNHLRVVKKPSLQVRNLFQAL
                     TRIRCIAFFMRNPVKAFHIDKEWNELGKPQISFDGFLNKIA"
     gene            complement(11539..13380)
                     /locus_tag="VGA21_12340"
     CDS             complement(11539..13380)
                     /locus_tag="VGA21_12340"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9651343.1"
                     /translation="MTEILFTFIGLFKDSELIYLISTVLWIFIAAVVGIDRLIRSHLQ
                     ADKLILKKIETESENLRLQILLLKSDFESLKSDFKLKSRFYANITHEFRTPLTLLLGP
                     LEEAFQKDTKSFSKTELILMLKNSRKLLRLINQLLEISKIEWGGSGLTIIRGDITELL
                     STITSFFLSMAEKRQVDFIRNMPLENPLCYFDPEKIEKIVTNLLSNSFKFTTPQGQVF
                     FNVDFSYSPNGDQQEIDNIIISVSDTGIGIDSEKIPKLFDLFYSSDNREGREYEGSGI
                     GLALVKELVEKHLGNIQVVNQIVGVKFIVTLPVNKRYFAESEITTFSFNANPKNINEL
                     EYLETPGIEIISKFDKNEFDVIKNSSLILLVEDNDDMRTLIRERFGKQFLIKEAKNGL
                     EGLAMALEIIPDLIITDLVMPRMSGIELCTFLKSDERTNHIPVIMLTGKSAVEERIKG
                     LETGADDYLIKPFNINELQVRVNNLIDQRKKLKERYERDLKIPRNEFSRVISKDEKFL
                     DRAAKAVERNIANSHFSVEDFQKEMGMSRMQLHRKLKALSEKSTSEFIRLIRMKRAAE
                     MILNGQGTISEIAYEVGFNDPSYFTKCFRNEFGIPPSEYKDYMNGLE"
     gene            13590..14063
                     /locus_tag="VGA21_12345"
     CDS             13590..14063
                     /locus_tag="VGA21_12345"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX9651344.1"
                     /translation="MEIFLHSNLKFFREKFGLSQEQIADLIEKKKSVIGHYERGTSFP
                     PLDSLVRISTFFGITLDNLTMADLRRQDEFILKFLDFNYQGFPELFKEPEATYENDRL
                     EDPTISADEKLEILKNQLKKEREEKSFLHNSLKNFANLQGIIMRRYDDLYYSRMK"
     gene            complement(14065..18927)
                     /locus_tag="VGA21_12350"
     CDS             complement(14065..18927)
                     /locus_tag="VGA21_12350"
                     /inference="COORDINATES: protein motif:HMM:NF013524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HEX9651345.1"
                     /translation="MISRILTSLIAFQLFMGHTYHSVGQNPDWMVPGKSYYRIPVSQD
                     GMYRITSEDISKSGLPIAAIDARNIQIFHRGNEQAITVTGQNDTRLDPQDFIEFYGQK
                     NDGTLDGDLYTIKELQPHIYYNLFSDTTAYFLTWGVGNERGKRIQMQSEFNSQGLVKE
                     QFVWKERINLFTNEYSRGYSLQNQISSSRFVQGEGFSGSRINAGSQIEIEVESGIKKS
                     GGPNGILEIQILGRNSEFHEVEIWIGQNLSSLRLAGSFNSFAYSPKITQLELIDSDIN
                     DGGKLLVRVKNINNNRANQVSVSYIKTKIPVEPNADAQNQFFQLEQKATEKTYLELSG
                     ISQETEVYDVSNPDSPIKLGLAYQDGILKGVITNTGNGNKLFVVSSRFLISKIAKVEN
                     RPLVNLLNDYIIITHEILRKPGESFTDPISEYAKYRSSASGGGFNPIIVDVNEIFNHF
                     SYGERTPLGIFNFLTQLSDEIPRNIFLIGKGLETSVNPERTTAALAFNNLVPTSGSPG
                     SDIIYSSGLTGLSSIPKFATGRLSASTSYEVESYLKKIIVAESIKVPRLFQKEILHLS
                     GGVKPEELSTFKSHLDSMMSIASNSPVGAKVQTLQKKSTKDYEFIDITQKINEGVGLV
                     TFFGHSASNLLDIDVGFVTNPELGYDNYGKYPIFYMNGCNIGSVYGNSYIQSEDWVLA
                     KNKGAIAVMGLSNLGAEQYLSVYGNLFYKIAFDEPSFYGKGLGEIQQELNRRYESLFG
                     TSYYAETHLEQMVLLGDPAVKLVPYSLPDYAFQKSAISLTQPNGELNTNEDIKVQIAI
                     SNNGILSTSPLQIAIRRRLQDGSPELLGPYSFIITGTSQSLEILIPNKNSKYAGFNEL
                     EIVLDPENQINEIEENNNFYNHNFYVKDRIPLKVNPYPYALLPSHNFEFVLLNKSGDN
                     SFITIAPENNFSNPIEISLQNENEIARSFWDSGADGISTYYWKTSSDNEEWSSVSSFT
                     LQTNDESRGWTQRHPAQISENSIEGITIDEQNNWTWQASELSIHLETIGGGGNYTIED
                     VVLELNSTAYIYSSRLCSNNTLNLLAFDRDSLVPYLASSEIPQCGRTPQIINSIHDET
                     LQTFPDLFLNALTNIAEGDFVLLFSIGTLNFDLWPADIFAELEQFGLGSPDASMLIPG
                     NSLFFVGKKGGSATQLMYGDSEVLLSTDEILSSQNSEGTIMTQLIGPASYWNSFQFTV
                     SDIMPGEFTFDIVAIDTLGNSTDFIKGIKNETDLRTLDAAKYPNIKLKYFTKGDEPRL
                     LNDWTVLYTPQPEGHLSYLGNSFNASPGFSVDEGRQLSVEFAFTNLSQENYSDSLEVY
                     LEQTVAATETRRSQMRIASPPPGETIIFNVDLTTMGMVGENSFFIEVNDSFHAEKNSL
                     DNKVQLFQFFTVVPDDIDPVIDVFVDGKHIINGDHVSSDPEITINYWDSNPYLIRENL
                     DNFIINFGTFCDTCALSRVELSSSSIHYYLEPGKNILKIDYKPGKLTRGKYLLEIFAP
                     DMKGNSPEFRYELLFEITNSETRNIKVQPAPNPFNKSLNFIFDIEAQEIPSGISLAIF
                     NARGQLIFEEEITLYSTLKVGLNRIEIPWKISNEPGIYYYKVRVIDDEEWMLAPAGNS
                     GSQGIIIFVR"
     gene            19175..19516
                     /locus_tag="VGA21_12355"
     CDS             19175..19516
                     /locus_tag="VGA21_12355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009582738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEX9651346.1"
                     /translation="MNLENTQVQMRKGILEFCILHIISRGEVYASDMLEELTSAKIMV
                     VEGTLYPLLTRLRKAGLVEYKWVESKSGPPRKYYWLTKQGSEFLLNLNETWKELIEST
                     KAITNKKVEKS"
     gene            19526..21811
                     /locus_tag="VGA21_12360"
     CDS             19526..21811
                     /locus_tag="VGA21_12360"
                     /inference="COORDINATES: protein motif:HMM:NF022441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEX9651347.1"
                     /translation="MKKNISINIGGIIFHIEEDGYERLKEYLDSINKYFSTFEDSSEI
                     IADIESRIAEIFLAKLNEGKQVITVEDVGSLISTMGSIQDFQAVEESDLFASQGTEKE
                     KGQDKKSGDRETPRKLYRDGKRKLLGGVASGIAHYFSIDPLWIRLIFVLLVFDVFLTF
                     SVSSILILSYIIMWIVLPESRDLPEDKKIKKLYRNPDDRVLGGIASGVAAYFGADVVV
                     VRLLFVLSIPIGGILVYIILWIILPEATTITDKVLMKGEPVTLSNIESNIKSSLNVKE
                     GEENLLIKILLFPFRLIAMLLEAIARILGPLLGFLVDVVRIVAGVVFSATGVCLLIAL
                     TITLGLLVGMFSETQYMNMDGLPIDLLKESFPVFGLVAAFIAAAIPALAMILGGIAII
                     IKKRTVHPSIGWSLFAIWMISIATMAIAAPGIITEFRSEGNYVREINYDIKDKIAVLD
                     LNETGMDDYHVTMLRLRGHDGDQYRLVQEFESRGRSRKEATDNAQMIEYNVQLQDSVL
                     LFDSNIQFKNNARFRNQRLNMTLYIPYNQPFIMTERMQHIIYNTLYRYDYRVSDIDDD
                     NRWIFTREGLECTTCGGLSYDQSDRNYTRTYSNHGFTELRLNGPFQVFVSPGEYSVEI
                     TGSQRQIRRARVNQSRDRLSIIFNDERIGSADNRILKVNIVLPELEQIRLSGKNISTV
                     NGFTCQQLKVQLDRESESSLDIQADEVLVTLLGKSRLNISGHAKDLDADIGPGSEFIH
                     QNFTSTNSSINYAGKSAIRDN"
     gene            21937..22518
                     /locus_tag="VGA21_12365"
     CDS             21937..22518
                     /locus_tag="VGA21_12365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009583298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX9651348.1"
                     /translation="MKLTIDKSATEDLLIEGCLQNSPSAQKAVYERYSPKMYAICIRY
                     VGDSESAKDILIQGFLKVLNKIGQFKREGSFEGWIRRIIVNESLTYLRKNKFMHLEVD
                     LETAEREPDYNFVQNTFEADELIALVNLLPPGYKTVFNLFAIEGYSHKEIAEMMGINE
                     NTSKSQLSRARKHLQELLLKREINVDLKFTGNE"
     gene            22511..23218
                     /locus_tag="VGA21_12370"
     CDS             22511..23218
                     /locus_tag="VGA21_12370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651349.1"
                     /translation="MSNHRIDNIFKNKLEGQQVNPPAEAWPAIHSQLKGRRINRIPIH
                     KIAASLLLIGLAAYSGYMITSSDNIIRGSGVGQTITSETKPAPKIEAGSTSNLPALIE
                     DESKSKLPETENFNKLHHFNLELLQPVAGIGNQLTTINQIEIIKQVSYEKTLRKPLVK
                     VEEERLPAITISYKSGNKMLMDDATSEPEKESTLRRAWDYALSVKNGEERPLNIKEMK
                     NELFASDFKKKKSKSQD"
     gene            23223..24308
                     /locus_tag="VGA21_12375"
     CDS             23223..24308
                     /locus_tag="VGA21_12375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651350.1"
                     /translation="MKKITVLLVCFLLSAAAVMSQSEEPVSRKDTIIISFANGSRLLL
                     LINKKDDIELIKRYDINAIISDLSQEINGLREDEDLVINEEEGDKYLLKPVVVEKSDF
                     QPADESEKTEEENKEKKDYENEGDYDSDDSKNDSYDVRINKKRKYWPRTRQSINIDIG
                     LNNYLEDFKFPDENNKPYSVKPHGSVYFGINTTYKTKLTGPLFVEWGGGFDWYNFNLD
                     NKSIRFVKTDSVVRFDPDPRTDIDPIRSKLNAGYIYLKMVPMLDFSYNSRSRLWNYHG
                     DGFRLGLGGYAGFRITSRTKFVYKMDGDREADKDSSNIFLNNVRYGIRFQVGYRGVDM
                     FLNYDVSPLFSENRGPELNAFSFGFTL"
     gene            complement(24360..24833)
                     /locus_tag="VGA21_12380"
     CDS             complement(24360..24833)
                     /locus_tag="VGA21_12380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651351.1"
                     /translation="MTKEEGKKYLERLNLIFYSIIALPLCAFAWFYLENHAGRHEVQV
                     NEQLRQSLQYVFPFLILVISGFGILIPGNQIKKINNSGNLKDRLGEFQRILLLRYALF
                     EIACILSVFAYYLTVEKFYAVFFVGTIVLFSLNRPTPFRIISNLRIKGEDKEIFQ"
     gene            24918..25505
                     /locus_tag="VGA21_12385"
     CDS             24918..25505
                     /locus_tag="VGA21_12385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017731602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX9651352.1"
                     /translation="MLINEKASRKHQIESEATQMFRSKGYAGSSMRDLAANLGIEAAS
                     LYSHVKSKEEILQKICFRIAQNFLEVLQKVLADKKRPIESLREAIIMHCLVMTRDVSA
                     SAVFWNEWKHLSGKSKEEFIQMKTKYEDVFISLISEGINSGEIRSIDSKFAALTILSS
                     LNCIHTWYKPDGNLAPRQVGEELAGMLLSGIQNKK"
     gene            25511..26500
                     /gene="paaA"
                     /locus_tag="VGA21_12390"
     CDS             25511..26500
                     /gene="paaA"
                     /locus_tag="VGA21_12390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009581974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaA"
                     /protein_id="HEX9651353.1"
                     /translation="MYGGGNIFEPINQNELLKDQDPIKLAEFESRIERGEKIEPADWM
                     PKLYRKQLIRMIEQHAHSEIIGALPEGTWITRAPGFKRKMALMAKVQDEVGHAQLLYS
                     AAETLGKSREDMISDLITGKSKYSNIFNYPAYTWADSCFISWLVDAGAIVNQVANSKG
                     SYGPYCRALERICIEESFHLKYGHHCVIHMATGTEKQREMMQQAINRWWAPMMHFFGP
                     PDKMSVHTEILMKWKVKMASNDECRQQFLDMYVPKIWELGLTVPDPLLRKNEETGVWE
                     YTEPDWEEFKRVINGDGPCNKERLAVRRTAEERGQWVRNALLKAENVYAIPLA"
     gene            26507..27124
                     /locus_tag="VGA21_12395"
     CDS             26507..27124
                     /locus_tag="VGA21_12395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013452429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation b"
                     /protein_id="HEX9651354.1"
                     /translation="MDSLDPRINRIAIPEKWDGDFKKSSLDQLETFEVFVQPKENKAF
                     QHEGIVHASDLELAFLFAKEQFSRRYTCSGMWVCKTSDVYVSPYTEDGKTVYEIKLPV
                     EQNGAAEKPYEIFHLYKRGKQHKHVGTVKAKSHINAIRNAGINIEEAKPVLNIWVIDS
                     SKIYANKTEDVDIWNTLPDKQFRDAIAYRGAEKIKEYKSRVKNNE"
     gene            27117..27863
                     /gene="paaC"
                     /locus_tag="VGA21_12400"
     CDS             27117..27863
                     /gene="paaC"
                     /locus_tag="VGA21_12400"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009581976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaC"
                     /protein_id="HEX9651355.1"
                     /translation="MSEAIRDLLYKMADDELILGHRNSEWTGLGPMLEEDIAFSSMAQ
                     DKVGQSLAFFELLHSLGESEPDTVAFTRNANQFHNCQFVELPIGEYDFSLVRHFLFDN
                     ADYLRFSHLKDSAYTPLSDLAKKFMGEVKYHLMHGEIWMKKLANGNEESLTRLQKSLD
                     YAFPFALGIFEKSEFEDQLITDGIYNGEEFLKNLWLEKIQNQISKTPLQLDTSVEAVF
                     GGRKGMHTEHLQPLLDEMSEVFKVDPGADW"
     gene            27863..28345
                     /gene="paaD"
                     /locus_tag="VGA21_12405"
     CDS             27863..28345
                     /gene="paaD"
                     /locus_tag="VGA21_12405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018478406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaD"
                     /protein_id="HEX9651356.1"
                     /translation="MIITEEKILHWLEEVKDPEIPVLSLIELGVITGVDIVDSHVKVS
                     MTPTFAGCPAIDYMKKDVIRVLHEKGIENVEVEVSFKSQWNSNMITDKGRKALEEFGL
                     APPPRHDFIIDIDILQHVKCPYCRSEDTEVKSPFGPTLCRSIHYCNGCKQAFEQFKPI
                     "
     gene            28401..28997
                     /locus_tag="VGA21_12410"
     CDS             28401..28997
                     /locus_tag="VGA21_12410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651357.1"
                     /translation="MKRKLFIELFLFIAFIAMVSGCNNAKKAREAAELDSLKQFYLVV
                     KDSLDSTWNFMIAEDDLKLTFMKRLLEEITHSNSYDEETVNSLVSRIEDARLMRYDRQ
                     SMGNQNLIDEYDFAINSLIKEITALARSNARYQNFPSMAQLIEYIQETDERVLLHRVH
                     YDFFAKDYNKFINEHGELAKEIDGGDPQNKVNLFQISE"
     gene            29001..30089
                     /locus_tag="VGA21_12415"
     CDS             29001..30089
                     /locus_tag="VGA21_12415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009581984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkane 1-monooxygenase"
                     /protein_id="HEX9651358.1"
                     /translation="MNLGKKIGFFSAFILPFLLLLGSYPQGYGYYLPLIFSFVFLPII
                     DFLVGEDKTNVPDDKIAFISKEKYYKIVLFIWVFVQTGILTWCFYHITYKHLETYEWI
                     GFILGASYVTGGIGITVAHELGHKNSKLQRFYSKVLLMQVCYMHFIIEHNQGHHVHVA
                     TPVDPATARKGENFYAFWVKSVFMGYFHAWQIEIKRLEKSGINKVSIQNRMIWYTLAP
                     ILFAGIFTLFFSIVSGRIVFEIPIFFFVQSLFGFTLLEHVNYIEHYGITRKEIEPGKF
                     ERVTPQHSWNANHLLSNFFLFQLQRHSDHHINATKPYQVLKQYDESPQLPAGYPTMVI
                     LSLLPPFWFRVMDKRLENHLQLAGKSVA"
     gene            complement(30201..31049)
                     /gene="fdhD"
                     /locus_tag="VGA21_12420"
     CDS             complement(30201..31049)
                     /gene="fdhD"
                     /locus_tag="VGA21_12420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002701469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HEX9651359.1"
                     /translation="MSKPSVFNVKVDRFSENQTERKIDLVAVEEPLEIRLGFGKKEER
                     VQRAITVTMRTPGNDYELATGLLFNEGIIKSYDDIADIHFCQDTGKQEEAENVVRVEL
                     AYEITPNLLPLERNFYTNSSCGVCGKTSIDSLEIRDCAIIPPAKPVLSRATLTSLPEK
                     LRKSQSVFEHTGGLHASALFRPDGDLVIMREDIGRHNALDKLIGAMVFERKIPLYNYL
                     LLVSGRASFELVQKSVVAGIPIMAAVGAPSSLAVDLAERFGITLVGFLKENYFNIYSH
                     PSRVIA"
     gene            complement(31049..32719)
                     /locus_tag="VGA21_12425"
     CDS             complement(31049..32719)
                     /locus_tag="VGA21_12425"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004155987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEX9651360.1"
                     /translation="MKSDLQIAQAAKPLRIDEIAGNLGIVEKDLEYFGQFKAKVPLHY
                     INKEKWQKGNLILVSAITPTPPGEGKTTTSIALADGLNKIGKKAVVVLREPSLGPVFG
                     MKGGAAGGGYSQVIPMEDINLHFTGDFSAIEKANNLLAALIDNNIQNLENNLGLDPRT
                     VTWKRVMDMNDRALRHVVTGLGGKSNGFLREAGFDITAASEVMAILCLSEDLMDLKNK
                     MGNIYVGDTFGGKAIYARDLKANGAMTVLLKDAIKPNLVQTLENNPAIIHGGPFANIA
                     QGTNTVVATKMGLSLTDYVVTEAGFGADLGAEKFLDIKCAYSGLSPKTIVLVATVRAL
                     KYHGGQKREELLTPNMDALKKGSLNLEKHLENMNHYGVPAVVAINRFVSDSREELEFL
                     KQLCINKNVRVAECRGWEAGGDGATDLAKQVIDNIKDCKSDFTPVYDWNLSVEEKIET
                     VARKIYGAGSISYLEKAKLDLNKVKRLGLEKLPVCIAKTQNSLSDDTKLLGRPEGFKI
                     TVREIEIAAGAGFLIPITGKMLRMPGLPARPAAENIDIDKNGNVIGLS"
     gene            32840..33532
                     /locus_tag="VGA21_12430"
     CDS             32840..33532
                     /locus_tag="VGA21_12430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651361.1"
                     /translation="MNPNVPHFISYMFVLITAATVYMVYYGVRLTDKKIAGKVLLIII
                     AWLILTGILSASGFYLEVEKQPIRFFLAAPAIVLTIIILFIIPSTRKFLKGLSLEVFT
                     YLSAIRIPVEITLWWLYLYNQVPVEMTFEGRNFDILAGITAPIIAYFVFTKKKWSTNI
                     AYTWNIVSLLLLVNIIAHAFLSLPTPFQQLAFENPNWGILYFPYLWLASFVAPVVLFT
                     HLVSIYQLRKGS"
     gene            complement(33541..35268)
                     /locus_tag="VGA21_12435"
     CDS             complement(33541..35268)
                     /locus_tag="VGA21_12435"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015812984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HEX9651362.1"
                     /translation="MSDVNMITREKRKFLPEDFTVKNWAQLLPYFQDLLERKIDNKAN
                     LMNWFIDRSELESILSEDLAWRYIKMSCDTENGEKRKSFEFFVNEIEPKVAPISNDLN
                     KKAVESPFFNEIDDSGYKIMIRNMVKQIEIFRDENIPLKTKLQTESQKFGQISGAMTI
                     EIDGKELTLQQAAVKLQSIDRKLREEVYNKISLRRLEDREKLDDLFTLLVRLRDEIAR
                     NADFANFRDYMFASMGRFDYEPQDCFDFHESIRSEVVPLLNEQAADRKKNLSFATLKP
                     WDKAVDILGRQALVPFTNSNELVERTIACFDALDPFLGQCLRIMREMNHLDLESRKGK
                     APGGYNYPLAEIGVPFIFMNATSTLRDMVTIMHEGGHAVHSFLTRKLELGDFKNPPSE
                     VAELASMSMELISMDHWNLFFQKKEDLKRAKIEHLEQIIETLPWVATIDKFQHWIYEN
                     PTHNHDERRVVWNKIFQQFSDSITDWTGLEENRNYLWQKQLHLFEVPFYYIEYGMAQL
                     GAIAVWKNYKENHIKGLKGYMDALKLGYTKTIPEVYAAANIKFDFSKTYISELMAFVK
                     EELHKLKAE"
     gene            complement(35278..36375)
                     /locus_tag="VGA21_12440"
     CDS             complement(35278..36375)
                     /locus_tag="VGA21_12440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009193736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HEX9651363.1"
                     /translation="MSDSGGIAANKTNSHYYNLKVKEVVRETEDAISIVFDPEVNIDY
                     KSGQFLTIILPFDGKKVRRAYSLCSSPFTDSNPAVMVKRVEGGLMSDYLNTRLKAGDI
                     LEVMEPMGNFTTEFSIENKRHIIMFGGGSGITPLISIIKSTLNQEPESILTLIYCNRN
                     IDSVIFRDQLENLQKHNEGRLQVIHILDDAPVNWQGPSGLLNHEMLQQLIERIPNWGP
                     EKTVYLMCGPEGMMNNVETLLEKRGIPREKIFKESFVAGTIGKEEKQDSAMEEGEGKD
                     YDVTVIYDGEEYTFEVKAGQHILETALDRDIDLPFSCQSGLCTACRGKLISGKIKMDE
                     DEGLSEAEKSEGYVLTCVGRPISSDVKIQIG"
     gene            36518..37855
                     /gene="purB"
                     /locus_tag="VGA21_12445"
     CDS             36518..37855
                     /gene="purB"
                     /locus_tag="VGA21_12445"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019944822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEX9651364.1"
                     /translation="MDLNSLSALSPIDGRYRNQTKTLAPYFSEIGLFRYRVRVEIEYF
                     ISLCKIPLPQLTKFDKNKFDDLRKIYEKFSEEDANHIKDIEKTTNHDVKAVEYFLKNC
                     FEELGLKNFSEFIHFGLTSQDINNTGIPLSLKEFMEFDFNPLLSEVADLINHCSKLWE
                     KIPMLARTHGQPASPTFVGKEFLVFKVRLEQQRSLLESIPYSAKFGGASGNLNAHKVT
                     YPDINWDAFADTFVEQLGMKRSFPTTQVEHYDNLAALFDNVKRINTIFIDLCRDLWLY
                     ISFGYFFQKIKDGEIGSSAMPHKVNPIDFENAEGNLGFANAIFEHLSSKLPVSRLQRD
                     LTDSTVLRNIGVPFSHTVVAYRSLLKGLGKLQLNKEAIDSDLQDNWVVVAEAIQNILK
                     REGHSKPYEILKDLTRKSEKITEQEVKNFIEQLKVSDKVKEELKKITPFNYTG"
     gene            complement(37859..38272)
                     /locus_tag="VGA21_12450"
     CDS             complement(37859..38272)
                     /locus_tag="VGA21_12450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651365.1"
                     /translation="MRRVAYLEYIFLGVFLTGLCSFTFAQDKPPKTNKIDQNPRVDIS
                     KEGPKAKKYLEILKSDTQGILYGNLCFEEFTRSKGYIYVIQPQPNPIFKARFKKSWHN
                     FGVKLIITLKHSIFWKVVEKNKTRECRQKTGDRVG"
     gene            complement(38340..39794)
                     /locus_tag="VGA21_12455"
     CDS             complement(38340..39794)
                     /locus_tag="VGA21_12455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009582714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX9651366.1"
                     /translation="MNKLSILIICIISSSPVYSQERTILTLEEVVRMAKQQSTAAILA
                     ETIRENRYWQFRTYKSNYAPRLALDGTLPDFNHSVTPVVQPDGTYSYPSVNRMNSDLT
                     LSLSQAIAPTGTQVFLNSSISRFDNLETNAQVYGGNPLEIGFSQPLFQFNQLRWDRRI
                     EPLRYEESRRKFAEDLESISQTASNLFFDLLIAQISLDIARKNVESNDTIFKIAQGRY
                     ELGKIPENELLQLELNLMNSRQAVAESELNFETSQLRLRSYLGITDENLVLIVPATVP
                     EFAVDEQVALQQARNNRQEAVAFDRRKLEAERDVSRAVGESGLDASLFGVFGLSNQAT
                     DFPGVYNNPGDQQRVRLGFFIPIVDWGRQKSRKKTAEANLKLVDFTVQQDEQNFDQEV
                     LTQVRTFKMLKDQIVITQKADEISLRRYDISKNRYLIGKISITDLSLALTEKDNAKRT
                     YITSLRNFWGAYYNLRRLTLHDFATNQILYLPED"
     gene            complement(39787..41148)
                     /locus_tag="VGA21_12460"
     CDS             complement(39787..41148)
                     /locus_tag="VGA21_12460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020531489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9651367.1"
                     /translation="MSERFIANIYIALEAVFANKMRSILTALGIIFGVAAVIAMLAIG
                     NGAQQEILEQIKLVGVNNIVITPVVEQSEGAVEEDVATGKEKKKFSPGLTLRDAESIR
                     KIVPGIERLSPEILIETYIIKSGLRRSAKLVGVQPEYFEVANFVLSDGKMFNDEHLKR
                     GDAVCIIGKGINTKFFPTDNPIGKSIKCGSQWLEIIGVLEERLISENAISNLGIRDYN
                     MDVYIPIQAMLIRYENRDLLTSTGVQAGARISRGNVIIIGSSSTDEPTTQENYHQLDR
                     LVIQVGETELLNPVADIVARMIQRRHFEVVDYEITIPELLLKQQQRTKSIFNFVLGAI
                     AGISLLVGGIGIMNIMLASVMERIKEIGLRLSIGAQKKDIILQFLFEAIMISITGGLI
                     GVMLGVGIAIGVSRIAEIPTIITFSSIILSFGVAATVGLIFGLAPARRAANQDPITSL
                     RHE"
BASE COUNT        12601 a         8101 c         7706 g        12867 t
ORIGIN      
        1 tttagatcat tccaggcaaa caaagaggaa ggccagtcag aacggagctg ggattccaaa
       61 aaaccaaaat caatctccag attgatccct tcaaaactag cttgcggctc aaacttaagt
      121 gcctataagt ttgttgaaat tggaagtggg ggacctatac acttccggaa ggatgaaatt
      181 gaagctcaat tctttcctaa tccggctaaa gattacatta caataaagca taatatatct
      241 ccgctaaaaa agccctatgt aaaaatctac gattcacaag gaggggagct ggtggttgat
      301 aaagctcttt cttatgaaga aaatcaaata aatatatcgc atttggcatg cggaaattac
      361 gtaattcata ttaattttaa tgggggagtt tttattagtc aattgataaa agaatgaaga
      421 aggcaattgt tctttttata ggattggctc tggttaattc ctgcaatcct tttacaggta
      481 agccagaaaa tatttatgct tactttgttt tcagtcaatc agaaggagat tttttcaact
      541 cagaacctct atatgatgaa acggaattaa gagtggccaa cccaaatata ccgggaaagc
      601 atccctttat tgaagtgaaa gaaagggatg gttttcatca gtttggcttg agtgggtgga
      661 cagttccaat aacttatttt gattatggca atggagatgt ggatactctt gcggtttccc
      721 tcgatccaga ttttgatttg ccaatgagta atttcaatcg gcttaagagt gtgaaatttt
      781 atttgaataa tattctaatc gaagagtggg atttcaagaa tcatcccgaa aaaagagatt
      841 ctatcttgat gtttaatacc gaagaagcac gattgaatgg atttaatagt gaggtggtta
      901 taagagtgcc aaagtctcca gatctagatg aacttaatta gttccgccac ggggagcaga
      961 tgtctatcac cctacataaa ttttactttg aattaagatt cttacgccat cagatgcata
     1021 cctaccattc aatatttttc ttatcaagaa aagcctcaat acctttcctg caatctttac
     1081 tctctcttgc ttttgcgttc aattttgccg cataatccaa cgcctcctct tcattcatct
     1141 tttgtacctt atgaattaat tgctttgtca ttttcatgga ttctccgctg ttatccgaaa
     1201 ctaatttctt cgcaaaatta agtactgatg attcaagttc atttgcctct acaaccttat
     1261 aaacaagtcc aaaattaacg gctgtttcgg cgtcgataag gtctccactc aacaataact
     1321 gcttagctct gccctcaccg gctttcttta tcagaaaaag agtaactatt gctggaataa
     1381 agccaatctt cacttcggtg tatccaatct ttgcctggtt agatgcgaaa acatagtcgc
     1441 atagggtaat tataccggca ccgccggcaa tggcatgtcc atgcatttgt gcaataacaa
     1501 ttttgtctga ttgataaatt ctgtaaaaaa gattcttcag ttgagtagag tcctcaagat
     1561 tctcttcaaa agtattttgt tgcaattgct tcagatattc caaatcagct ccggcacaaa
     1621 aaacatcgcc ttctgccttg atgatgacga ccttagtacg tttatcctta tcaattttgg
     1681 caaatgtttc gtctagttcg cgaacaaatt gattatttaa tgcatttcgc tttgctggtc
     1741 tgcttagaat tacggttgca attctttgtt caacagaata tttaacaaat tccattattt
     1801 gccttcattc aaatttactg gaatatataa ttaattaaca ttaatgatag tacatccata
     1861 aggtaaaaat tctactaatg aagaaaacac caatgagtgc acacgaataa atcagccaac
     1921 tgtactttct ttttaaccag aaaatgctca aactacaaat taacaaatag agcaaaatca
     1981 cctcttttgt accgaaaact ctggtggaaa taacatttaa aggtaattta tttaatagct
     2041 caacagatag caacatcata tttacaaact tgtccaatag ataagccagt ccctgttgaa
     2101 aaaaatcaat aaacccaatt gcaataaata agattgcacc atttagaatt ataaaaacag
     2161 caggaattac cagcaggtta cttaagagga aataggtggg aaattgctta aaataatata
     2221 acgataatgg caaggttcca atttgtgcag caagtgaaat agccaacagg gtccataact
     2281 ttggaggaat ccaaacaggt tgagctcggg ttaactttgg tccaaaaaat aatatagaca
     2341 gaacagcaag gtaagacagc tgaaaaccca ccgaaaagag caatccaggc tcaaaaagca
     2401 aaatcataaa tgcactggca aatacaaggt tgaaagcatt acttcgtcta tcaatcaact
     2461 ttcccaaggc caatatactg aacatggtaa ctgcccggac caccgaaggg ctaagtcctg
     2521 tgataagggc ataaaaccaa agttgtatta caatgaaggt gattatcaaa tatttccatc
     2581 ttgtatttat taatttattg aaaatgaata aaaatattcc ataaaatatt ccaacgtgta
     2641 aaccagaaac cgccaaaaca tgcatggtgc ctgaattgct aaaattctct ttcacatccg
     2701 gatcaagatc gctcttctga ccaatggtta gggccttgag aactccaccc gactggctgt
     2761 ccccgaaata tttgtcaata gtttgctcaa tttgaattcg ggtgttaaag gcaagacttt
     2821 gatgtaatgg gatatctctt acactttttg caacaacttt ctttggagta atatattgag
     2881 ttaagtaaat accttgtaac gcgtaatatg ctttgaagtc aaactctaaa ttatcattaa
     2941 aatttttaat ggtatcagga tgggatttta ccagcagaag atctcccagt ttcccatcaa
     3001 catcttccgt aagagtaact aaaatcctgt ggttacattt cctccagttc ccatcaattt
     3061 gaaatccgat aatttctgca attcccctgt tacgctcgcc ctttttatcc aatggtttat
     3121 ttaaccagag taagaaactt tctgattcca aaagttttgg tgaattaagg tgggggtttt
     3181 ctgtttgtaa ttgattggag taaatactcc atcctcccaa ataaatggtt agcagaccaa
     3241 gtatgccaac cagatggcta ttgctgaata attttttcct gaaactaatg gcaaccccta
     3301 aatacgctga caacaaaatg aagtaggaga aatcatgagg aatggcggca tatctgcatg
     3361 ataatatacc taaagaaaaa accagggtta acctggcaaa cggcatgggc gctagttctt
     3421 gcaccccaca atataattat attaataact agttgtcttt tttgattatt gttttttgat
     3481 atgcttcaac aatatctcgc acaattttat gcctgacgac atcttttggt tgtaattcaa
     3541 tgaaaccaac cccgggaata tccttcagaa tacctattgc ttctgccaaa ccggagttct
     3601 gattcttcgg taaatcaatt tgggtcatat ctcctgtaac aattactttg gaactcggcc
     3661 ccatccttgt aaggaacatt ttgatttgca tcggagttgt attctgtgct tcgtcaagaa
     3721 gaatgaaagc attgtttaat gttctgccac gcatatatgc caatggagca atttcaatga
     3781 cgcggctttc cagataaaat ttcaatttct caaaaggaac catttcatcg aggctgtcat
     3841 atatcgggcg cagataggga tcaatctttt cctttagatc tccgggaagg tatccgagat
     3901 tttctccagc ctctacagcg ggtctggtta taattatttt ttttacttcc ttattcttaa
     3961 gagcctgaac agccattgca accgaaacaa aggtctttcc ggtaccagca ggtccaacaa
     4021 caaaaataac atcattctca cgcacgctgt aaaccagttt ttgctgatta attgttctcg
     4081 gctttattac tgacccgctt ggaccgtata tgagaacttc atcttttgaa gggatttctt
     4141 tttcttcgtt aatgtttgaa gtcaaaacat cctgaatatt tgtatgagta attttaccgt
     4201 acttatgata atgttccagc agggaattca gtatcgcatt gattcgaaga atctctgctg
     4261 agtttccttt aatccttatc tcattgcctc tggaaattat tttactttta ggaaatgccg
     4321 atgccacttc tttgatattc tgatcttcaa ttccaagaaa atctatcagg gaaatatttt
     4381 caagtgtgat taccttttct accaaacgtt gcagatttga atttaaatta cttgatcttt
     4441 aaatggttga aaattaatta tttttactca caaatttagc aaatttatta tcctttcatg
     4501 gccatcatca catttacatc ggatttcgga gaaaccgacc attacattgc agccgtgaaa
     4561 gccaggatcc ttgcatttaa tcctaattta accatagttg acataaccca caacgtgcaa
     4621 gcaggagatt tagcccatgc ttcttttgtg gtgaaatcag tgtatagaga ttttccgaaa
     4681 ggaacaactc acctgattgc tgtgggttca gcaaaatccc gatttgtggg tttaaaggtt
     4741 aatgaacatt attttcttgg gcatgacaac ggcttatttg gattgattac tgaagaaaca
     4801 agcactgaag caatagattt gaacaatcct cacaagatac gaagtcagtt cccggcaaag
     4861 gatatattag ccactgcagc ggcaaaattg gcaagtggag ttggattttc agacctgggc
     4921 gtgcgaattg atgattttaa gataatgata ccccgcagag tgaaatcaac aaaatctcaa
     4981 attgccgggc atgtgatcag ggtagattac tacggaaatc ttatctcaaa tattgaagaa
     5041 gagacttttc gtttcctcac cagggataag cgatttgcaa tatcctttgg tcgcgaacga
     5101 gcttttcatg ttcatgatta ttatgattca gttgagaacg gtgagtgttt cataattttt
     5161 aacagtctgg ggttacttga aattggaatc aacaaaggca atgccgcgca gctgcttggg
     5221 cttgattatg atagcccgat aattattaat attgaacaat aatgctgaca cgaattgtaa
     5281 taatgaattt tgaagaggag aatattcaga gcttcctgaa aatctttgaa caggctgaga
     5341 ataagatccg gacactacct ggctgtattc acctggaatt aaaaagaaat gtgcataatc
     5401 attcccaact ggcaactatt agcaaatgga attcagaaaa agagttaaat aactatcgtg
     5461 attcagaatt ctttaaggac acctggcaaa aaaccaaagc attgttttca actgatgcgg
     5521 aagctttttc ttttgtagat gttgatctta aataattgta aaaatgggca tcatgcaata
     5581 tgtttgcgtg ctttagagcc gaagtggcgg aattggtaga cgcgcacgac tcaaaatcgt
     5641 gttccttcgg gagtgagggt tcgattccct ccttcggtac aaaaaaggcc tgtattaggc
     5701 atttttctat tcaacaagaa gtttggtttg atatcttttt aaaacattac ttacaattac
     5761 aaaatgaatt cctttattga gatcctggtc caagtcgagg ttaatcgaat taccgctata
     5821 tacaacttcc ttccttatta ttgtatttcc agtgggtccg atgagcgtaa tactcagagg
     5881 atcgccatta attgcaattt ccggattcat atcaatggtt atttttccaa tgatgccgga
     5941 tgaaacagga ttaggataaa ttaccggcgt gctggccaac aaatcattct tttctaccga
     6001 aaccaccttt gaataggtat acttaccact aaaatctatt tgtttgagac ggtaataatt
     6061 gacaccattt aaaggtctgt cttttataat gtaaaaattt tcttcagaag ttgacccatg
     6121 accctttacc tcagaaattt tcagaaattc cctcccattc atggacttct ctatttcaaa
     6181 acgatcattc tcaatttcgc ttgaagttat ccaggaaatc tctgcaaact tatttttcaa
     6241 ttcagctttg aaatcagtta aaattaccgg cagtggattc acaccccctt tacttccgaa
     6301 agagaccggg ctaaaacttg agagataggc tgttatcgaa ccttcgcctc cacctggtcc
     6361 cacgacactt ccttcaccaa ctgcgccgaa catgtcttcc caccggctcg aagtactatt
     6421 ataatgcgca aatgttaaat cttcctcatc cgtaatctca ctaaaatcag catctttcca
     6481 aaatagagta acctccgccg aaattccttc agaaggactc acatcccagt attcaatacc
     6541 actgatggtg gccagagttg gatcaatatc atcactattc ggaactgagc caatgaaata
     6601 ttcagcagta gtggttaagg ttgaagcgcc gccagtaatg ttactcacac taaggggcga
     6661 ccaccggtcg attgttccat tactatcgcc taatggaaac tcaaaatctg tagttccgtt
     6721 tttctgcaca ggtccatcaa taaatctctg attacctgac cctgtggaaa ttccaccttc
     6781 cctaaatatg attttcgctg tggaagaagt ttttattatt ccactcatga aatcaagttg
     6841 gtttaagtta acatccgaaa ttaaggtgag ttgaggaatc gttgggctgg tattattggc
     6901 aacaacccgg ttgaaataaa taccgtccgt gccggctcca gtcaaagatt gagcaggaat
     6961 aacctgaggt gagttaccat taaatgtcat gtaactttga tcattaaaac taatatcacc
     7021 gaagtttaat ggtcgtaata gttcaccgcc taaagtaagg gaagagttat tgaacaggaa
     7081 gagcgttgca ttttgctgat ttttactgga aattgtaaaa tctcccatca catttaaatt
     7141 tgaattatca agcagatata gtttattcct ttccgttgtt aaaccgaagt ccgtatcaat
     7201 agtcaattgc gaatttccac ttacaactat ggctgcatct tgtttaagtg ttgttgtcat
     7261 cgcggaagaa gaatgagata aaatagccgt atgttttaaa ttaaggttac ccatttgaag
     7321 cgtgcctgca cttccggcca caattccccc cagagtaaag tttgaaatac ctctcttatc
     7381 ttcccaggta aaattaccgc caatagttcc gtcaatatct ccatcaaatg ttagaaaaag
     7441 ctccttgcta tcggaacttg cattttgata gatatttaca tcattgccca caattaattt
     7501 cgcatttgta aatgcaacac cggatggact taaattactt ggattaaaag tgagattgaa
     7561 atttcctcct acggataata atgcgggatt caaaagggtg ccggtaagat taatattgaa
     7621 tctccggtac agggcgttta taattaggtc tttgccaatg gataactcgc catttatttc
     7681 aagtatggtt gatcccgcag tagtattatc ggcataaata gcgacacttc cgtttgtaaa
     7741 attcatgctg gcattggctt ccacatctaa attaaaaagg gaacccccgc cactgtcggt
     7801 ctttgtcaac ccattcggac cgttcacaat taaattggat tgatctcttg cccttattgc
     7861 aatattggat gttcctccac tttttgtgac ggatatatct ccatttaccg taccggtact
     7921 gttgttccag agctcaaaaa gtattgtacg gcccaattgt ttatttatta ttatgtttcc
     7981 cagaatactg aagctcgaaa tatcctcaga ttcaaaccgt aataagtttg atgctgctcc
     8041 catctggttc attgttaaat cgccggcaat ggtaattgtg gaagccccca gtgattcaac
     8101 gtggatttga ggggtatacg cacttacatc aacatcctgt tgcataacca gagaaccaac
     8161 gttcagttta gcattatcca gagttcttac ctgcaaaatg tactgaaatg ttgataatgt
     8221 tgtagcctca tcctggatca ggttcatcgt tccggaaacc gacagttcac tattaccaaa
     8281 taattggatt tctgtgatga attccggaaa gaaaattgtt ccatctgtcc aaccacctgt
     8341 taaataattg taattcagat cccctgctac gcttgtcgtt cctcccgtca taaacaatct
     8401 acagccaatg tcttgtcctt cattctccct gataaaattc atattcccgc caatatctaa
     8461 gagagcgcca tcggccataa taatttcaac ttccttcaga atacccggcg ccatttccaa
     8521 ctgaacatcc cgtaccacag aaagacccgc accagcgtac atttccaccg tggccgtata
     8581 attctctttg ttattaatta gcaacgaatt aattatgtga gccgtgttta ttataacatc
     8641 acggtttatt attacatcat ccgaagaagc tggaacacct gccggactcc agatagccgg
     8701 atcattaaag ttgccatccg ctatggttat tttttgactt attgcataga atgaaatagc
     8761 aagcaataac ccaaaggata tgaatgtttg cttaataatt cttgttttca tatttccagg
     8821 tagtttagac cactatatta atttaatcca taataagttt ttctatttaa atagatactt
     8881 acttattaat gtaatattat tagtattagt agactttttt taattttact aaatggtact
     8941 tttagtggat gaaatatgta aaacatcatt ttaataatgc tttgaagcac tttatccttt
     9001 ccgcaatatt tctggctttc agcggaaatg atgtaatcag tcaatataaa tctcctgccg
     9061 gaagtctgaa ttttggaatt tccacttacc aggggatatc gtttttggag ggagcccagt
     9121 tcacagatat caaaccagga tatgcacagg ccagcggatt aaatattaaa tattatcaaa
     9181 ctatcacaat aggtctccag gtagatattt tatattcaaa ggctgcagga accttttcta
     9241 aaatagaagc actctcaaat caaaataatc tcaaaattaa aactaaacag aaacttaact
     9301 ttatatcggt acctatttca ataaaccttt atctttcaga taggtttgaa tctgaaaaca
     9361 ttcacattaa gctcggagtt gttccttcat ttttaataaa agcagtgaag tcaagtaata
     9421 atccggagga aaacggagat aaagttgaaa attccaatga tttcaggtcc atggactttg
     9481 gatttcttgc aggtattggt gtggaagtga gaagatttaa tctgattggg gaatattatc
     9541 tgggagtata taatttaaac gccaacaaga atggttctcg aataaccaat catggaccca
     9601 gaattggatt aaattatttt ctaaaaaggt gggattgatc aatcgggata attacttcaa
     9661 accgggttcc cttgtctact tcactctcca ttaaataaga tccacccatt ttcttaacgg
     9721 cagtatcaaa caaatacaga cccaatccgc tccctcgaga ttttgtgcta gccttataga
     9781 acatttgaaa gatattttgc ttatagatat tttcaatccc tattccattg tcttcaattg
     9841 taataaaaat aaactctgta ttatcgtaaa cttcgattga aattaaccca ggtctgtgtt
     9901 ctgatagaaa attgcaggaa ttgtcaatta gagaatgcag gatggtctgt aaggcaaatt
     9961 gatttccaat gaaggtgttt ttaatgatgc ttttattaat ttttaccact tttgaattta
    10021 gatcctgata tgaggcaagc atatccgcta caatattatt gaaagtgaat tcttcctgct
    10081 cgtagtcact aaattccagg actgaatgtt tacccagcgc gattacaata ttatttaacc
    10141 tctctacctg actttgaaag agactaaagt aatcaagaga cttagcttca ttaatttctc
    10201 tgtgtaccaa attggagagc cccatcatgc tggcaattgg gcctctcaag tcatgtgaaa
    10261 ctctatatat aaatctatta agttcatctc ttttttgaga gaaacttgat tcaatagcct
    10321 ttttggaagt attatccctt aaaattaaag taaacatttc tccttcatat tcattcgagt
    10381 gacttagaag caagtagctc aacggaacag aaacattctc gttattttcg agatgtgttt
    10441 cgcctgtaat acaaccttgt tcaatagctt cattcaacct tattttggga aactttgtgg
    10501 acagtactct tttcaaccct tcattttcaa gctcgtaatt tctgaagaaa tgccttgccg
    10561 gtttatttag gtatttaaat gaataattaa aatctatctt aataaccatt tcaaaccctc
    10621 tttcaactat atcctgaaag taggtattat catgatccag agatatttta ctttgtctca
    10681 acttcgcagt ttaagcaatc ttgtttaaga atccatcaaa cgaaatttga ggtttcccta
    10741 attcattcca ttctttatcg atatgaaatg cttttaccgg atttcgcata aagaatgcta
    10801 tacatctaat ccgggttaaa gcctggaata aattcctaac ctgcaggctt ggttttttta
    10861 caacccgaag atgatttctt gttttggctg taacctcatt caaatcgatt ttttcaggac
    10921 cttgcacaaa gtatgtaata ccattaaact gatttttcat tttaatggct ttgtcaatct
    10981 gtcttccgat atcacttgca gaaattccgt aactcccggc tagtaattcg cgtgaaaggt
    11041 ggatattggt tttgaatgac gaaagaagca ggtcctcaaa aatgaatgaa gatatgatta
    11101 ctgcaaattt acagctgtgt tgattgtcag ggtttgataa ttcaatgcta ttattcctgc
    11161 cagctcgcct tagaataaga aatgtaatgt tttttttgtt agctgcgcat ttaatagcct
    11221 gctgaattag tctattttca gcagtagaag gctctccgta aagattaata aaaattgttt
    11281 caaaagagcc atttgagatt aagtctctaa ctgagcatat tccgaccgtt tttaccagtg
    11341 atggttgact aatggcattc ctttcaggac atccaacaat tgaaacatga ttgccggctg
    11401 acagaagctc gttaactatc gcattcatga cagggccact ttttcctaat gccagtattg
    11461 ttttggtcat cagaacaaaa gtattctggt aaattcaggc tcagtggtaa tttaatccct
    11521 aaaacttgta caattatatc actccagtcc attcatataa tctttgtatt cagaaggtgg
    11581 tataccaaat tcatttctaa agcatttcgt aaaataagaa ggatcattaa atccaacttc
    11641 atatgcaatt tctgaaatgg ttccctggcc attcaaaatc atctcagcgg cccttttcat
    11701 tcttattaat cgtataaatt cactggtgga tttttctgac aaagccttta gctttctatg
    11761 caattgcatt cgactcattc ccatttcctt ttggaagtcc tccacagaaa agtggctgtt
    11821 ggcgatattt ctttcaaccg ctttggcagc tctatcgaga aacttttcat ccttactaat
    11881 caccctgcta aattcatttc tagggatttt tagatcccgc tcatatcgct ccttgagttt
    11941 ttttctttga tcaatcaggt tatttaccct tacctgcaat tcattaatat taaatggttt
    12001 aatcagataa tcatccgcac ctgtttcgag gcctttaatc ctttcttcaa ctgcagattt
    12061 gccggtaagc attattacag gaatatgatt agttcgttca tcgcttttta gaaatgtgca
    12121 tagttcaatt ccactcatac gcggcattac aagatctgtt ataattaagt caggaattat
    12181 ctccagagcc attgcaagac cttcaagtcc attctttgct tctttaatta gaaattgctt
    12241 gccaaacctc tccctgatta atgtacgcat atcatcattg tcttctacca atagaatcag
    12301 agaagaattc ttgataacat caaattcgtt tttatcaaac tttgaaataa tttcaatccc
    12361 gggagtctca agatattcaa gttcattaat gttttttgga tttgcattaa atgagaatgt
    12421 ggtaatctca ctttcagcaa aatacctttt gttaacaggc aacgtaacta taaactttac
    12481 accaactatt tggttaacaa cctgaatatt ccccaggtgc ttttcgacca attctttgac
    12541 gagggccagc ccaattccac ttccttcgta ttctctccct tctctgttgt ctgagctata
    12601 aaacaaatca aatagttttg ggatcttttc cgagtcaatt ccaattccag tatctgaaac
    12661 agaaattata atgttatcaa tttcctgttg atccccatta ggtgaataag agaaatcaac
    12721 attaaaaaat acctgcccct ggggtgtagt gaatttgaat gaattggaca ataaattagt
    12781 aactattttc tcaatttttt caggatcaaa ataacacagg ggattttcaa gtggcatatt
    12841 cctgataaaa tctacctgtc ttttttcagc cattgataaa aaaaaggaag tgattgtgct
    12901 aagaagttcg gttatatccc ccctgataat agttaaccca gaacctcccc actcaatttt
    12961 ggatatttca agcagttggt ttattaacct caacagtttt cttgaattct ttagcattaa
    13021 gatgagttca gtcttgctga acgattttgt atctttttgg aatgcctcct caagcggtcc
    13081 aagaagtaat gtaagagggg tcctgaattc atgagtaata tttgcataga atcgagactt
    13141 aagtttgaaa tcagacttta gactttcaaa atcacttttt aatagcaaaa tctgtaatct
    13201 taaattttca gattccgtct caattttttt aaggataagt ttgtcggctt gaagatgaga
    13261 tctaataagc ctgtctatcc cgaccacagc agcaataaag atccataata cagtacttat
    13321 caggtagatt aactcagaat ctttaaaaag gccaatgaaa gtaaatagaa tttctgtcat
    13381 aatttttcaa aactaaaagg gagttatagt tgtagacatt ggaggctgag ccatcacgtt
    13441 cagaaaccaa gcatactttt aaacctggac ggtattgtcc atggtaaatt tttttttgaa
    13501 ttaattagca atgtctcgta tatttttcgt acaattattt ctgattatat tataaaaata
    13561 tcacagttat attacaaatt ccaaaataaa tggagatttt tttacattcg aatcttaaat
    13621 ttttcagaga gaaattcggc ctatcgcaag agcagatagc cgatttaatt gaaaaaaaga
    13681 aaagtgtgat tggtcattac gaaagaggaa cgtcttttcc tccccttgat tccctggtca
    13741 gaatttccac tttctttgga attaccctcg acaatttaac tatggcagac ctacgaagac
    13801 aagatgaatt cattctcaaa tttcttgatt ttaattacca aggctttccg gaattattta
    13861 aagagccgga ggctacctat gagaatgacc gacttgaaga tcctaccata tcagctgacg
    13921 aaaaactaga aatcctgaaa aatcaactta aaaaagaaag agaggaaaaa agttttctcc
    13981 ataacagcct caaaaatttt gcaaatttac aaggtattat catgcgacga tatgacgatc
    14041 tgtactattc aagaatgaaa taaattacct tacaaaaatt attatcccct gggatccgga
    14101 attacctgca ggagctaaca tccattcttc atcatcaatc actctcactt tatagtagta
    14161 tatacctggc tcatttgaaa ttttccatgg gatttcaatt ctattcaaac caacctttaa
    14221 tgtagagtat agagtaattt cctcctcaaa aattagttgt cccctggcat taaaaattgc
    14281 taaagaaata ccagatggta tttcctgagc ttcaatatca aaaataaaat tcaaactttt
    14341 gttaaatggg ttaggagcgg gttgaacttt tatgtttctt gtttctgaat tcgtgatctc
    14401 aaataagagc tcatacctga attcgggtga attgcctttc atatccggag cgaaaatctc
    14461 caaaagatat tttccccggg ttaacttgcc aggtttataa tctattttca gaatattctt
    14521 cccaggttca agatagtaat gaatagatga agaagataat tcaactctgc tcaaagcaca
    14581 ggtgtcacaa aaagttccaa agtttattat gaaattgtca aggttctccc ttataagata
    14641 tggatttgaa tcccaataat taatcgtaat ttcaggatcg cttgaaacat ggtcgccatt
    14701 tataatgtgt ttaccgtcaa caaatacatc aattaccgga tcaatatcgt caggaacgac
    14761 tgtaaaaaat tggaacaatt gaactttatt atccaaggaa ttcttttcag catgaaatga
    14821 atcattcact tcaataaaaa aactgttctc accaaccatt cccatagttg tcaggtcaac
    14881 gttgaaaata atagtttcgc caggtggagg ggaagcaatt ctcatttgcg accttctcgt
    14941 ctctgtggca gcaactgttt gttccaaata gacctccagc gaatccgaat aattctcctg
    15001 agatagattt gtaaatgcaa attccacact tagctgcctc ccttcatcaa ccgaaaagcc
    15061 cggcgatgca ttgaatgagt ttccaagata acttaaatgt ccttctggct gtggtgtata
    15121 caaaactgtc caatcattca gaagcctggg ctcatctcct tttgtgaagt acttgagttt
    15181 aatattgggg tacttggcgg catccagggt tctcaaatcc gtttcattct taatgccttt
    15241 aatgaagtct gttgaatttc cgagtgtatc aattgcgaca atatcaaatg taaactcgcc
    15301 tggcataata tcgcttactg taaattggaa actgttccaa taacttgccg gaccaataag
    15361 ttgtgtcatt atagtgcctt ctgaattttg acttgaaagt atttcatccg tacttaataa
    15421 tacttcagaa tcaccataca ttaactgagt ggctgacccg ccttttttac caacaaagaa
    15481 taaggagttt ccaggaatta acattgaagc atcaggagag ccaagaccaa actgttccag
    15541 ctcagcaaat atgtcagctg gccataggtc aaagttgagg gttcctatgg aaaagagaag
    15601 gacaaagtct ccctcagcaa tatttgtcaa agcattcaga aatagatcag gaaaagtttg
    15661 caaagtttca tcatgaatgg aattaataat ctgcggagtc cggccacatt gtggaatctc
    15721 tgaactggct aagtacggca ccagtgagtc tctgtcaaat gccagcaaat tcaaagtatt
    15781 gttcgagcac aatctcgaag agtatatgta tgctgttgaa tttagttcca gaacaacatc
    15841 ctctatagta taattaccac ctccgccaat ggtttcaaga tggatactga gttccgaagc
    15901 ttgccaggtc cagttatttt gttcatcaat tgtgatcccc tcaattgaat tttcagaaat
    15961 ttgcgccgga tgtcgctgag tccaacccct ggattcatca ttagtttgta aagtaaagga
    16021 agaaacacta ctccattctt cattatcact tgacgttttc caatagtagg tgcttattcc
    16081 atccgctccg gaatcccaaa agcttcttgc aatctcgttt tcattttgaa gagagatttc
    16141 aattggattt gaaaaattat tttccggtgc tattgtgata aaagaattgt ctccactttt
    16201 attcaatagg acaaactcaa aattatgtga gggcaaaaga gcataggggt atggatttac
    16261 tttcaacggt atccggtcct ttacatagaa gttgtgatta tagaaattgt tattttcttc
    16321 gatttcatta atttgatttt cgggatccag aactatctcc aattcattga atccagcgta
    16381 tttagaattc ttgttaggaa tcaggatttc taagctttga gatgtcccgg ttattatgaa
    16441 tgaatatgga ccaagtagtt caggagatcc atcttgtaaa cgtcggcgta tcgcgatttg
    16501 taacggagat gttgatagaa ttccattgtt gctaatcgcg atttgaactt ttatgtcttc
    16561 atttgtattt agttctccat ttggctgagt aagggaaatt gcactttttt ggaaagcata
    16621 atccggaagt gagtaaggaa ccaacttaac ggcgggatca cccaataaaa ccatttgttc
    16681 aagatgagtt tctgcataat aggacgttcc aaaaagcgat tcgtatcttc gattgagctc
    16741 ttgttgaatt tcacccaatc cctttccata aaatgacggt tcatcgaatg ctattttata
    16801 gaataagttc ccatatacgc tcaggtattg ttcggcgcca agatttgata atcccattac
    16861 tgcaattgcg cctttgttct ttgccagaac ccaatcctca gactggatat aggaattacc
    16921 gtacactgat ccaatgttgc agccattcat gtagaagatt ggatacttac cataattatc
    16981 ataaccaagt tcggggttag taacaaatcc aacatctata tctaacaagt tgctcgcgga
    17041 atgtccaaaa aaagttacca ggcctacccc ttcattaatc ttctgggtga tgtcaataaa
    17101 ttcataatcc tttgttgatt tcttttgaag agtttgaacc tttgcaccaa ccggagaatt
    17161 agaagcaatg ctcatcatac tatcaagatg gcttttaaat gtcgacaatt cctcaggctt
    17221 tactcctcca cttaaatgca aaatctcctt ctgaaataac cttggaactt tgattgattc
    17281 agccacaatg attttcttca aatagctttc cacttcataa gatgttgatg cggataatct
    17341 tccggttgca aatttgggga tagatgataa acctgtgaga ccactggaat aaattatgtc
    17401 agagcctggg cttcctgaag ttggaaccag gttgttgaat gcaagtgcag cagtagttct
    17461 ttcaggattt accgacgttt ccaaaccttt cccaataaga aaaatatttc tcggaatctc
    17521 atcagaaagc tgagtaagga aattaaaaat tcctaatgga gtcctttcac cataactgaa
    17581 atggttaaag atttcattaa catcaacaat tatcggatta aagccacctc ctgatgctga
    17641 acttctgtat ttagcatatt ccgagatggg atccgtaaaa gattcacctg gctttctcag
    17701 aatttcatga gttataatga tgtaatcatt gagcaaattg acaagtggcc gattttcaac
    17761 cttggcaatt ttgcttatta gaaacctgga actcaccaca aatagtttgt tcccatttcc
    17821 ggtattggta ataactccct tcaaaattcc gtcctgataa gctaatccaa gttttattgg
    17881 ggaatcagga tttgatacat cgtaaacctc agtttcctgg cttatgccgc ttaattcaag
    17941 gtaagtcttt tcagttgctt tctgttcaag ttgaaaaaac tgattttgtg catccgcgtt
    18001 tggctcaact ggaatcttag tttttatata tgataccgaa acctgatttg ctctattgtt
    18061 attaatgttt tttactctta caagtaactt gcctccatcg ttaatgtcag aatcaattaa
    18121 ttccagctgg gtaattttag gtgagtaggc aaaactatta aatgagccgg ccagccggag
    18181 cgatgaaaga ttttgcccta tccatatttc aacttcatga aattctgaat ttctgccaag
    18241 gatttgaatt tcaagaattc cattagggcc cccagacttt ttgattcctg attctacttc
    18301 aatttctatt tgacttccgg catttatcct gctgccggaa aaaccttcac cctggacaaa
    18361 ccttgatgag gaaatttgat tctgtaaaga ataacccctg gagtactcat ttgtaaaaag
    18421 attaattctc tctttccaaa cgaattgctc ttttaccaaa ccttgagaat taaactcaga
    18481 ttgcatctga atcctctttc ctctctcatt tccaacgccc caggtaagaa aataagcagt
    18541 tgtatctgag aatagattat aatatatatg aggctgcagt tctttaatag tatacaaatc
    18601 tccgtccagc gttccatcat tcttttgacc ataaaattca ataaagtctt gtgggtccag
    18661 tcgggtatca ttttggccag ttacggtaat agcttgttca tttcccctgt gaaaaatctg
    18721 aatgtttctg gcatcgattg cagcaattgg taatccagac ttgcttatat cttcagaggt
    18781 aattctatac attccatcct gagaaacagg tattctgtaa taacttttac caggaaccat
    18841 ccaatcagga ttttgaccca ccgagtggta agtatgaccc ataaaaagct ggaaggcaat
    18901 taatgatgta agtattcttg agatcacagt acaatgatat aatcatatac aatatatata
    18961 taaccatatt aactcaattt cttacattta gggactccag cgtaatttat tatattatta
    19021 atcttataaa ggacagtaat ttatctttta cacagacata atataatttt tcttagataa
    19081 ttgttgaatc gtgattattt tgcgtaaggt acttggccat acctagtact tttccgtata
    19141 caatagctag ggtattataa ataactctta tggtatgaat ttagaaaata cacaggtaca
    19201 aatgagaaag ggcattttgg agttctgcat tcttcatatc atttcaagag gcgaagtgta
    19261 tgcttcagat atgttggagg aattaacttc cgcaaaaata atggttgttg aaggaactct
    19321 atatcccctg ttaacgcgac tgcgaaaggc ggggcttgtt gagtataagt gggtggaatc
    19381 caaatcagga cctcctcgta aatactattg gctaacaaaa cagggttcag aatttttact
    19441 taatttgaat gaaacctgga aagaacttat agaatcaacc aaagcaatta caaacaaaaa
    19501 agtcgaaaaa agctaatcac cagacatgaa aaagaatatc agtataaata ttggcggaat
    19561 aatttttcat attgaagaag atgggtatga aagactaaag gaatatttag actccataaa
    19621 taagtacttt tcaacctttg aagacagttc agaaattatt gctgatatag aaagcagaat
    19681 tgctgaaatt tttcttgcca aattgaatga aggtaagcag gtaataacag tcgaggatgt
    19741 cggatcccta atctccacga tgggtagcat acaggatttc caggctgtgg aggagtcaga
    19801 tttgtttgca agccagggaa cagaaaagga aaagggtcag gataagaaat ccggtgaccg
    19861 agaaactcca cgtaaactgt acagggatgg caaacgaaaa ttgcttgggg gcgtggcctc
    19921 cggtatcgcc cactattttt ctattgatcc actttggatc cgcttaatct ttgtgctttt
    19981 ggtttttgat gttttcctga ctttttctgt atccagtatt cttatacttt cttatattat
    20041 tatgtggata gttctacccg agtcaaggga tttgccggaa gataagaaaa taaaaaagtt
    20101 atatagaaat ccggatgaca gggtgcttgg aggtatagcc agcggagttg cagcttattt
    20161 cggtgccgat gttgttgtag ttcgtctgtt gtttgtgtta tcaataccta taggtggcat
    20221 tttagtttat atcattctgt ggattatttt acctgaagct accacaatta cagacaaagt
    20281 gcttatgaag ggagaaccag taactctttc aaacattgaa tccaatatca agagcagtct
    20341 taatgtaaag gaaggggaag aaaatttgct gattaaaatc ttattattcc ctttcaggtt
    20401 gattgctatg cttttggaag caatcgcaag aatattaggt ccactgcttg gtttcctggt
    20461 tgatgttgta agaattgttg cgggggttgt cttctctgct acaggtgtat gcttattaat
    20521 cgcgcttact attacactag gtttattagt tggaatgttc tcagagactc agtacatgaa
    20581 tatggatggc ttacccattg atttgctgaa agaaagcttt cctgtatttg gattagttgc
    20641 cgcgtttatt gcagccgcca ttcctgcact ggccatgata ttaggtggca ttgcaataat
    20701 tattaagaaa aggaccgttc acccttcaat tgggtggtca ttatttgcaa tatggatgat
    20761 tagtatcgct acgatggcga ttgctgctcc aggaataatc actgagtttc gcagtgaagg
    20821 aaattatgtt agggaaatta attatgacat taaggacaaa atagccgtac tcgatctgaa
    20881 tgaaacagga atggatgact atcatgtaac aatgcttcgg ttgcgcggtc atgatgggga
    20941 tcaatatcgt cttgttcagg agtttgaatc aagaggtaga tccagaaagg aagctactga
    21001 taatgcccag atgatcgaat acaatgtaca actgcaggat tcagtcctgt tatttgatag
    21061 caatattcaa tttaaaaata atgccagatt cagaaatcaa agattgaata tgacattgta
    21121 cattccatac aaccaacctt ttatcatgac cgaacggatg cagcacatta tctataatac
    21181 actatatcga tatgactaca gggtttctga tatagatgat gataatcgat ggatttttac
    21241 cagggaaggt ctggagtgca caacatgcgg aggcctttcc tacgatcagt ctgatcgtaa
    21301 ctatacccga acttacagta accatggttt cactgagtta agattgaatg gaccctttca
    21361 agtttttgtt tctccaggtg aatatagcgt agaaattacc gggagccaac ggcaaatcag
    21421 acgcgcacga gtaaaccaat cccgagatag attatcgatt atttttaacg acgaaagaat
    21481 agggtcagct gataatagaa tactaaaggt taatatagta ttgcctgagc ttgaacaaat
    21541 cagactctcc ggaaagaata tctcaacagt taatggattc acttgccaac aattgaaagt
    21601 tcaattagac agagaaagtg aaagcagttt agatattcag gctgatgagg ttctggtcac
    21661 tttgttgggc aagtccagat taaatatctc gggccatgca aaggacttag atgccgatat
    21721 tgggcctgga agcgaattta tacaccaaaa ttttaccagt acaaattcat caattaacta
    21781 cgccggcaaa tcagctatcc gggataatta gcttatttct ccaattccgg gtaaaaggca
    21841 actttccctt ccaaatttca gttaatcttc atagacaaaa ttaactttgt ccatgcaacc
    21901 tatcgttcca atttcgcatc ttctatttgc tccggaatga agttaacaat tgacaaatcg
    21961 gcaacagagg accttttgat tgaaggctgt ctacagaaca gtccttcagc gcagaaggct
    22021 gtttatgagc gctattcacc gaaaatgtat gccatttgca ttcgttacgt tggggattca
    22081 gaatcagcaa aggatattct gattcaggga tttttgaagg tgcttaataa aatcggccag
    22141 tttaaaagag aagggagctt tgaagggtgg atacgaagaa ttatagtaaa tgaatcgctt
    22201 acttatcttc gaaaaaataa attcatgcat ttggaagtgg atctggaaac ggccgaaaga
    22261 gaacctgatt ataactttgt tcaaaacact tttgaggcag atgaattgat tgcgctggtg
    22321 aatttgcttc cacctggtta taaaacagtt ttcaacctat ttgctattga aggctattcc
    22381 cacaaagaaa ttgctgaaat gatgggcata aatgaaaata caagtaagtc gcagctcagc
    22441 cgtgcaagaa agcatctgca ggagttgctt cttaaaaggg aaataaatgt tgatttaaag
    22501 ttcacaggaa atgagtaatc accggattga caatattttt aaaaataagt tggaaggcca
    22561 gcaggtgaat ccacctgctg aggcatggcc tgccatacat agtcaactaa aaggaagaag
    22621 gatcaaccgc atacctattc ataagattgc agcaagtctt ttattgattg gtttagcagc
    22681 ctattccggt tatatgatta ccagctccga taatatcatc cggggttccg gggtggggca
    22741 aaccataact tcagaaacga agccagctcc aaaaattgaa gcaggttcaa cttcaaatct
    22801 tccggcactt attgaggatg agtctaaaag taagttacct gaaactgaga atttcaacaa
    22861 actccatcat tttaatcttg agttgctaca accggttgca ggaatcggta atcaattgac
    22921 taccatcaat caaattgaaa ttattaaaca agtttcttat gaaaaaactc tccgcaaacc
    22981 cctggtaaaa gtggaagaag aaaggcttcc ggcaataacc ataagttata aatcaggaaa
    23041 caaaatgttg atggatgatg caacctccga acctgaaaag gaatctacac tcagaagagc
    23101 atgggattat gctctaagtg taaaaaatgg agaagaaaga ccactcaaca tcaaagaaat
    23161 gaagaatgag ctttttgctt cagatttcaa aaagaaaaag tcaaaaagtc aagattaaaa
    23221 aaatgaaaaa aataactgta ttgcttgtgt gtttcctgtt aagcgctgcc gcggttatga
    23281 gccaatcaga agaacctgtt tccaggaaag atactattat tattagtttt gccaatggca
    23341 gccgtcttct gttattaatt aataaaaagg atgacattga attgattaag cgctacgaca
    23401 tcaacgccat tatatcagat ctctcacagg aaataaatgg attgcgtgag gatgaagacc
    23461 tggtgattaa cgaagaagaa ggagacaaat atctcctgaa gccagtggtt gtggagaaat
    23521 cagatttcca acccgccgat gagagtgaaa aaaccgagga ggagaacaaa gagaaaaagg
    23581 attatgaaaa tgaaggtgat tacgacagtg acgactctaa aaacgatagt tacgatgttc
    23641 gtattaacaa gaaaagaaaa tattggccaa gaacaagaca gtctataaat attgatatag
    23701 gcttaaacaa ctaccttgaa gatttcaaat ttcctgatga aaataataaa ccttactcgg
    23761 taaaacctca tggttcagtg tattttggta taaatactac gtacaaaacc aaattaaccg
    23821 gacccctatt tgttgagtgg ggtggcgggt ttgactggta taatttcaac ctggataata
    23881 aaagcatcag gtttgtaaaa acagatagtg tagtgaggtt tgatccggat ccaagaactg
    23941 atattgatcc aattcgaagt aaactaaacg ctgggtatat ctacctaaag atggttccta
    24001 tgctggactt cagttataat agccgatcca ggttgtggaa ttatcacggc gatggcttca
    24061 gacttggact tggcggatat gccggattca ggattaccag tagaacaaaa ttcgtatata
    24121 aaatggatgg ggatagggaa gctgacaagg atagctcaaa tattttcctt aataatgtca
    24181 gatacgggat caggttccag gttggataca ggggcgtaga catgtttctc aactatgatg
    24241 taagtccatt gtttagtgaa aatcgcggtc ccgaattgaa tgctttcagt tttggattta
    24301 cactttagtt agaaataaat ctttcaagaa gaaggcttca ggattttcct gaggcttttt
    24361 cattgaaaga tctctttatc ctcgcctttg attcttaaat tagaaatgat tcgaaaagga
    24421 gtgggtctgt ttaaagaaaa aagaacaata gttcctacaa aaaaaactgc gtaaaatttt
    24481 tcaacggtaa ggtaataggc aaacaccgat aaaatacaag ctatttcaaa caaagcgtag
    24541 cgtaaaagca aaattcgctg gaattcaccc aaacgatctt tcaggttacc tgaattattt
    24601 atctttttga tttgatttcc aggaattaga attccaaatc ccgaaatcac aagaatcaaa
    24661 aaaggaaaaa catattgcaa ggattgccta agttgctcgt tcacctgaac ctcatgcctc
    24721 ccggcatgat tttcaagata aaaccatgca aatgcacata gaggaagcgc aattatgcta
    24781 tagaaaatca ggttcaatct ttccaggtac tttttccctt cttccttggt cataccgtaa
    24841 gaattacagg cttcaattta ctaataattg catctttaat tattgctaac agtcgttagg
    24901 tttgttatct ttgtggaatg ctgataaacg aaaaagcgtc cagaaaacat cagattgaat
    24961 ctgaagcaac ccaaatgttc cgatcgaagg gatatgccgg gtcatctatg cgggatctgg
    25021 cagctaattt aggcatagag gcggcaagcc tgtattcgca tgtgaagtct aaggaggaaa
    25081 tcctgcaaaa aatttgtttt cgcattgctc aaaattttct tgaagtttta caaaaagtac
    25141 ttgcagataa gaagcggcct atagagagtc ttcgggaggc tattataatg cactgccttg
    25201 ttatgacccg cgatgtatct gcatcggcag ttttctggaa tgaatggaaa cacctgagcg
    25261 gaaaatccaa agaagaattt atccaaatga aaactaaata tgaggatgtt tttatttctt
    25321 taattagtga gggaataaat tcgggagaga tacgaagcat tgactctaaa tttgctgccc
    25381 tgacaatttt gtcttcactc aattgcattc atacctggta taaacccgat gggaatttag
    25441 caccaagaca ggttggagag gaattggcag gcatgttact ttctggaatt caaaataaaa
    25501 agtaaaaaat atgtacggag gaggcaacat tttcgaaccc attaaccaaa acgagctgct
    25561 caaagaccaa gatcctatca aacttgcaga gtttgaatca agaattgaac gtggggaaaa
    25621 gattgaacct gcagactgga tgccgaagct ttacagaaag cagctcatca ggatgatcga
    25681 gcagcatgca cacagtgaaa taatcggtgc gttaccggaa ggcacatgga taacgcgcgc
    25741 tcccggattc aaaagaaaaa tggctttgat ggcaaaagtg caggacgaag tagggcatgc
    25801 gcagcttctc tacagcgcgg cggagacttt ggggaaatcc agagaagata tgatctccga
    25861 tttgataacc ggaaagtcaa aatattccaa cattttcaat tacccggctt atacgtgggc
    25921 tgattcctgt tttattagtt ggctggtaga tgcaggggcc atcgtaaacc aggtggcgaa
    25981 ttcgaaagga agttacggcc cttattgcag ggcactggaa aggatttgca ttgaagaatc
    26041 gttccatctg aagtacggtc atcattgtgt aattcacatg gccaccggga ctgaaaaaca
    26101 aagagaaatg atgcagcagg ctattaatcg ttggtgggct cccatgatgc atttctttgg
    26161 acctccggat aagatgtccg tgcatacaga gatcctaatg aaatggaaag taaagatggc
    26221 ctcaaatgat gaatgtagac aacaatttct cgacatgtat gtacccaaaa tttgggagtt
    26281 gggtctcacc gtacctgatc cgttgctaag aaaaaatgaa gagactggag tttgggaata
    26341 tacagaacct gattgggaag agtttaagcg ggtaatcaat ggtgacggcc catgtaataa
    26401 ggaaagattg gcagtacgga gaaccgctga agaaagaggt caatgggtaa gaaatgcact
    26461 cctcaaagca gaaaatgttt acgcgattcc actcgcttaa aaagttatgg attcacttga
    26521 tccaagaata aatcgaattg caattcctga aaagtgggac ggcgatttca agaagtcttc
    26581 tcttgaccag ctggagactt ttgaagtttt cgttcaaccc aaagaaaata aagccttcca
    26641 gcatgaaggt attgtacatg cctcggacct tgaacttgcc tttctctttg ccaaggagca
    26701 attcagccgt agatatacgt gctccggcat gtgggtttgc aaaaccagtg atgtttatgt
    26761 tagtccatat acagaagatg gcaagactgt gtatgaaatt aaattaccgg ttgaacaaaa
    26821 tggtgctgca gaaaagcctt atgaaatttt ccatctgtat aaaagaggta aacagcacaa
    26881 gcacgttggc acagtaaaag caaaatctca cattaacgct attaggaacg caggaattaa
    26941 cattgaggaa gcaaagccgg ttctgaacat ttgggtaata gattcgtcca agatttatgc
    27001 aaacaaaact gaagacgttg atatctggaa tacgcttccg gacaaacagt tcagggatgc
    27061 tatagcttac cggggggctg aaaagataaa agaatataaa tcaagggtaa agaacaatga
    27121 gtgaagcgat aagagattta ttatataaga tggcggatga tgagctcata cttggtcatc
    27181 gaaattctga atggactgga ttagggccaa tgcttgagga ggacatcgct ttttcttcaa
    27241 tggcccagga caaagttggt caaagcctgg cattttttga actgttacat tccctcggtg
    27301 agtcagaacc ggataccgtt gctttcacca gaaatgctaa tcaattccat aattgtcagt
    27361 tcgtagaact acctattgga gagtatgatt tcagtcttgt gagacatttt ttatttgata
    27421 atgccgatta tctgagattt agtcacctaa aagactccgc ttacactccg ctttctgatt
    27481 tggcaaagaa atttatgggg gaagtgaaat atcatttaat gcacggcgaa atctggatga
    27541 agaaactggc aaacggaaat gaagaaagcc ttaccagatt acaaaaaagc cttgattatg
    27601 cgtttccatt tgccctgggg attttcgaaa aatctgagtt tgaagatcag ttaattactg
    27661 atggcattta caatggcgaa gaattcctga aaaatttgtg gctggaaaaa atccagaatc
    27721 aaatatcaaa aacaccactc cagctagata cttctgttga agctgtattt ggagggcgaa
    27781 agggtatgca tacggaacat ctacaacctc tactggatga gatgtctgaa gttttcaagg
    27841 ttgatccggg ggcagattgg taatgattat aactgaggag aaaatattgc attggcttga
    27901 ggaagttaag gatcctgaaa ttcccgttct tagccttatt gaattaggcg ttattaccgg
    27961 cgtggacatt gtggattccc atgtaaaggt ttctatgact ccaacctttg ccggctgccc
    28021 tgcaatagat tacatgaaaa aagatgtgat ccgggtgtta catgaaaagg gcattgagaa
    28081 tgttgaggta gaagttagtt ttaaaagcca gtggaactct aacatgatta ctgataaagg
    28141 caggaaagca ttggaggaat ttggccttgc tccacctccg aggcatgatt ttattataga
    28201 catcgatatt cttcagcatg taaaatgtcc ttactgcaga agtgaagata ctgaagtaaa
    28261 atcccccttt ggtccaacgc tatgccggtc tatacattac tgcaatggtt gcaaacaggc
    28321 attcgagcaa ttcaagccaa tttagagctg aaattttgtt gctttttttg ctacctttaa
    28381 attaagaatc aacacaatgt atgaaaagga aattattcat tgagcttttt ttgtttatag
    28441 ctttcatcgc catggtatca ggttgcaaca acgcgaagaa agccagggaa gcagcggaat
    28501 tagacagcct gaaacaattt tacctggtag taaaagacag tcttgattca acctggaatt
    28561 ttatgatagc tgaagacgat ttgaagctaa cttttatgaa gcgcttgctg gaagaaatta
    28621 ctcactcgaa ttcttacgat gaagaaacag ttaattctct ggtgtcaaga atcgaggatg
    28681 cacgtcttat gcgctatgac cgccaaagta tgggtaatca gaacttgata gatgaatatg
    28741 attttgctat caattccctg attaaagaaa ttaccgcgct ggcccgttcg aacgctcgct
    28801 accagaattt tccttctatg gctcaactca ttgaatatat tcaggagaca gatgaaaggg
    28861 tgttgttaca ccgcgtacat tatgattttt ttgccaaaga ttataataag tttattaacg
    28921 aacatggtga actggcgaaa gagattgatg gtggcgatcc tcaaaataaa gttaacttat
    28981 ttcaaattag tgaataagga atgaaccttg ggaagaaaat agggtttttc tccgctttca
    29041 tactcccctt cctattattg cttggttctt acccacaggg atatggctat tatctgcccc
    29101 ttattttttc attcgtattt ttacccatta ttgattttct ggttggtgaa gataaaacca
    29161 atgtgccaga tgataaaata gctttcattt ccaaagaaaa gtattataaa attgtcctct
    29221 ttatctgggt atttgttcaa acaggcattc taacctggtg tttttatcac attacttaca
    29281 agcatcttga aacctacgaa tggattggct tcattttagg agcatcttat gtaaccggag
    29341 gcatagggat aacagttgca cacgaacttg gacacaaaaa ttcgaaactg caacggtttt
    29401 acagcaaagt attgctaatg caggtttgct atatgcattt tatcattgag cataaccagg
    29461 gacatcatgt gcatgttgct acccctgtgg atcccgcaac tgccaggaag ggggaaaatt
    29521 tttatgcctt ctgggtcaaa tcagtattta tggggtattt tcacgcttgg cagattgaga
    29581 tcaagagact tgaaaaaagt ggaattaata aagttagcat ccaaaataga atgatttggt
    29641 acaccctggc ccccatttta ttcgcaggaa tatttacttt atttttttca attgtttccg
    29701 gaagaattgt atttgaaatt ccaatatttt tcttcgttca aagcttattt ggattcacac
    29761 tacttgagca tgtaaattat attgagcatt atgggattac acggaaggaa attgaaccgg
    29821 gtaaattcga aagagttact ccccagcact cctggaatgc taatcacctg ctgagtaatt
    29881 tttttctgtt tcaattacaa agacactctg atcaccatat caatgctaca aagccttacc
    29941 aggttctaaa acagtacgat gaaagtcccc agctgcccgc aggttatcct actatggtca
    30001 tactcagctt acttcccccc ttttggttca gagttatgga caagagatta gaaaatcatc
    30061 tgcaattggc cggaaagagc gtcgcttaaa taataaagat taggatttcg aagtgcttct
    30121 aaagaagttt atttgtcagg tatgatctgc ctggattaag gatcaaaata tattcatcaa
    30181 agaatattca ggaaaagttc tcaggcgatg actctggatg gatgcgaata tatattgaag
    30241 taattctctt taagaaaacc taccagggtg atgccaaatc tttctgccag atcaacagca
    30301 agactcgaag gagctccaac tgccgccatt attggaattc ctgccactac cgatttttga
    30361 acaagttcaa agctagcccg tccgctcacc agaagcagat aattatacag agggatcttc
    30421 ctttcaaaga ccatcgctcc aatcaattta tccaaagcat tatgcctgcc aatgtcttcg
    30481 cgcataataa ccaggtctcc gtcaggccta aagagtgctg aagcatgtaa acccccggta
    30541 tgctcaaaaa ctgactgtga ttttctcaac ttttcgggta aggaagttag agttgcccgg
    30601 ctcaataccg gtttggccgg gggtatgatt gcacaatcgc gaatttccag ggaatcaatg
    30661 gaagtttttc cacaaactcc acaacttgaa ttcgtataga agtttctttc gagaggcagc
    30721 aagtttgggg ttatttcata agccagttct acacgcacaa cattttcagc ttcctcctgc
    30781 tttcctgtat cctgacaaaa atggatatcg gctatatcat cataactttt gatgattcct
    30841 tcattaaaca acaaacccgt ggccaattca taatcattac ctggcgttct catagtgacg
    30901 gtaatagctc tctgaactct ctcctctttc tttccgaagc caagtctgat ttcaagaggt
    30961 tcttcaactg cgacaaggtc aattttcctt tcagtctggt tttctgaaaa tctatctact
    31021 ttaacattga atacagaagg cttagacatt atgataaacc tattacattt ccatttttat
    31081 caatatcaat attttccgcc gccggcctgg caggcaatcc cggcattcgc agcattttcc
    31141 cggttatagg gattaaaaat ccagcccctg ctgcaatctc aatttcccta accgttatct
    31201 tgaacccttc cggtctccca agaagttttg tatcatcaga taaagagttc tgtgtcttag
    31261 cgatgcaaac cggtaacttt tccaaaccta atcgttttac tttatttaaa tccagtttag
    31321 ctttctcaag gtacgaaatt gaaccggcac cataaatttt cctggcaaca gtttcgattt
    31381 tttcttcaac agagagattc cagtcgtaaa caggagtaaa atctgattta cagtctttaa
    31441 tattatcaat tacttgttta gccaagtccg ttgcgccatc acctcctgct tcccacccgc
    31501 gacattcggc cactcttacg tttttgttta tgcataattg ttttagaaat tccagttctt
    31561 ccctcgaatc acttacgaac ctatttattg caactactgc cggcactccg taatggttca
    31621 tgttctctaa atgtttttca aggtttagcg agcctttttt caaggcatcc atatttggtg
    31681 ttaacaattc ctcacgcttc tggccaccgt gatatttcaa ggcccgaaca gttgctacca
    31741 gtacgatagt ttttggtgaa agtccggagt aggcgcattt aatgtctaag aatttttccg
    31801 cgcctaaatc cgcaccaaag cctgcttcag tcactacata atcggttaga gataagccca
    31861 tttttgtggc gactaccgtg tttgtaccct gggcaatatt agcgaatggc ccaccatgaa
    31921 taatagcagg attgttttcc aaagtttgaa ccagattcgg ctttatagcg tccttcagca
    31981 aaacagtcat tgcaccgttt gctttaagat cccttgcgta aatcgccttc cctccaaagg
    32041 tatctccaac atatatattg cccatcttgt ttttcagatc catcaggtct tcggataggc
    32101 aaagaattgc cattacttct gaggccgcgg taatatcaaa gcctgcttca cgcaggaatc
    32161 catttgactt ccctcccagt ccggtaacca cgtgtctaag agctctgtca ttcatgtcca
    32221 tcactctttt ccaggttacg gttcggggat ccaagccaag attgttctct aaattctgaa
    32281 tattattatc gattaaagct gccagtaaat tgttcgcctt ttcgatggcg gaaaaatctc
    32341 cggtaaaatg aaggtttata tcttccattg gaataacctg ggaataacca ccacctgccg
    32401 cgccgccttt cattccaaac accggaccta aagacggttc tcttaataca acgaccgctt
    32461 tttttccgat tttatttaag ccgtcagcca gagcaataga agttgtagtt ttaccctcac
    32521 ccggaggagt tggagtaatt gctgaaacta aaatcaggtt tcctttttgc catttttcct
    32581 tattaatata atgtaacggt actttagctt tgaactgacc gaaatattcc aggtcttttt
    32641 ccacaattcc aaggtttccg gcaatctcgt caattcttaa aggtttagct gcctgagcaa
    32701 tctgtaaatc acttttcatt ttcaaggttt aggtttccta atcgttagtt ttagagaggc
    32761 taatattaca acattcattt gaaaaaataa tccaactgac ttggtggatt tatgccttta
    32821 ttatgtagaa atttgattta tgaatccaaa tgtaccccac ttcatcagtt atatgtttgt
    32881 actgattact gcagcaactg tttacatggt ttattatgga gttagactaa cagataagaa
    32941 aatagccggc aaagtattac taattattat tgcctggtta attcttaccg ggattcttag
    33001 tgcttcgggt ttttaccttg aagtagaaaa acagccgatt aggtttttcc tggcagctcc
    33061 ggcaatagtg ctgacaataa tcatcttgtt cataattcct tcaacaagaa aatttctaaa
    33121 agggttatct ctcgaagtat ttacctattt aagcgccatc aggattccgg ttgaaatcac
    33181 gctttggtgg ttatacttat acaaccaggt tcctgtggag atgacctttg agggaaggaa
    33241 ttttgatatt ttagctggaa taacggcacc aataattgcc tattttgtct tcaccaaaaa
    33301 gaaatggagt acgaatattg cctacacatg gaatattgtt agtctcttgc tccttgttaa
    33361 cattattgca catgcttttc tttcactccc cacacccttt cagcaattgg catttgagaa
    33421 tcctaactgg ggaattctat atttccccta tctatggctg gcaagttttg tagcacccgt
    33481 tgttttattt acccaccttg tttctattta ccaactcagg aagggatcat agccggcaaa
    33541 ctattctgct tttagcttgt gtaattcctc tttaacaaaa gccatcagtt cagatatata
    33601 tgtcttactg aagtcaaatt taatattcgc tgctgcataa acctcaggaa tagtttttgt
    33661 ataacctaat ttcagagcat ccatataacc cttcaatccc ttgatatgat tttctttgta
    33721 atttttccaa accgcgattg caccaagttg ggccatccca tattcaatat aataaaatgg
    33781 gacctcaaac aggtgaagtt gtttctgcca caaatagttc ctgttttctt caagaccggt
    33841 ccaatcagta attgagtctg aaaattgttg aaatatttta ttccatacca ctcttctttc
    33901 gtcatgatta tgggtggggt tttcataaat ccaatgttga aatttgtcaa tggtggctac
    33961 ccatggaagt gtttcaatta tttgttccag atgctcaatc tttgcacgct tcaagtcttc
    34021 ttttttttga aagaaaagat tccaatgatc catagagatc aattccatag acatagaagc
    34081 aagctccgct acttcagaag gtgggttttt gaaatcgcct agttccagtt tccgcgtaag
    34141 gaaggagtgt acagcgtgcc cgccttcatg cataatggta accatatccc tcaaagttga
    34201 ggtggcgttc ataaatataa atgggacccc aatctctgca agggggtaat tatatccccc
    34261 aggcgctttt ccttttctgg actccagatc caaatggttc atttcacgca ttattcgcag
    34321 acactgcccg agaaacggat cgagtgcatc aaaacaagcg atcgtccttt caacaagttc
    34381 attgctgttg gtaaaaggta ccaatgcctg acgaccaaga atatccacag ctttatccca
    34441 gggttttaaa gtagcaaagc ttaagttttt tttccgatct gctgcctgtt cattcaataa
    34501 tggaacaact tctgacctga ttgattcatg aaaatcaaag cagtcttgtg gctcgtaatc
    34561 aaaacgaccc attgaggcaa acatataatc tctgaaattg gcaaagtctg cattacgagc
    34621 tatctcatct cttaacctga ccaacaaggt aaaaaggtca tctagttttt ccctgtcctc
    34681 caatcttctg agtgatattt tattgtatac ttcctcacgc aatttgcgat cgattgattg
    34741 cagtttcaca gctgcttgct gaagagttag ctctttgcca tcgatctcaa tggtcatagc
    34801 accggaaatt tggccaaact tttgtgactc ggtttgaagt tttgttttaa gaggaatatt
    34861 ttcatcccgg aaaatttcaa tctgtttaac catgttccgg atcattattt tgtaaccgga
    34921 gtcatcaatt tcattaaaga aaggagactc tacagccttc ttatttaagt cattggatat
    34981 tggagcaact ttcggctcaa tttcgttcac aaaaaattca aaagactttc gcttttcccc
    35041 gttctctgta tcacaagaca ttttaatata cctccatgca aggtcttcag acagtatgga
    35101 ttccagttcg ctccggtcaa taaaccaatt catcagatta gctttattat caatcttcct
    35161 ttccagcaaa tcctgaaagt aaggcagcag ttgtgcccaa ttttttactg taaaatcctc
    35221 agggagaaat tttctttttt ccctggtaat catgttcaca tcactcatct tttattatta
    35281 acctatttga attttaacat cggagctaat gggtctgccc acacaggtaa gtacatatcc
    35341 ttctgatttc tcggcttcag acaggccttc atcttcatcc attttaattt tacctgaaat
    35401 tagttttcca cgacaagcgg tacaaagacc gctttgacag gagaagggca aatcaatatc
    35461 acgatctaaa gctgtctcaa gaatgtgctg accagctttt acttcaaagg tatattcctc
    35521 tccgtcataa atcacggtga cgtcgtagtc tttaccctcc ccttcttcca tcgctgagtc
    35581 ttgtttttct tctttaccta tggttcccgc gacgaaactt tctttgaaga ttttttccct
    35641 gggaattcct cttttttcta aaagcgtttc tacattattc atcatccctt cgggtccaca
    35701 catcaggtaa accgttttct cagggcccca attcggaatt ctctcaataa gttgctggag
    35761 catttcatga ttcaacaacc ctgaaggacc ctgccaattc acgggagcat catcaaggat
    35821 atgaataacc tgcaacctgc cttcattgtg tttctgcaga ttctccagct gatcgcgaaa
    35881 tatcaccgaa tcaatatttc tattacaata aatcagggta agaatacttt caggttcctg
    35941 gttcaatgtt gatttaataa tggagatcaa gggagtaatc ccactcccac caccgaacat
    36001 tataatatgg cgtttatttt caatactgaa ttctgtagtg aaattaccca taggctccat
    36061 tacttctaaa atgtccccag cctttaatcg ggtattcagg tagtcagaca tcaatccgcc
    36121 ttcaactctt ttcaccataa ccgccgggtt agagtcggta aagggtgatg aacaaaggga
    36181 ataagcacgc ctcactttct tgccatcaaa aggtagaatt atggttagaa actggcctga
    36241 tttatagtca atattaactt caggatcaaa tacaatggaa attgcatcct cagtctccct
    36301 tacaacttct tttactttga ggttgtagta atgtgaattt gttttatttg cagcgatgcc
    36361 cccggaatca ctcataattg tcttttagtt aaataagcca cttttcataa taagggcagt
    36421 agttgcaaat ctaaccatga agccagaaaa aagccttagt tatgttattt tatgttgagg
    36481 aaaactaaat ttgtgccttc ataaccttca cttcttaatg gatctgaatt ctctgtctgc
    36541 actttcacct attgatggcc gctatcgaaa tcaaactaaa acgcttgcgc cttacttttc
    36601 agagattgga ctgtttcgat acagggtgcg ggttgaaatt gaatatttta tttccctatg
    36661 taaaattccc ctgcctcaac taacaaaatt cgataagaac aagtttgatg atttgaggaa
    36721 aatttatgaa aaattttccg aggaagatgc gaaccacatc aaagacatag aaaagacgac
    36781 caaccacgat gtgaaggccg tagaatattt tctaaaaaac tgttttgaag agttgggctt
    36841 aaaaaacttt tccgaattta ttcatttcgg tttaacgtca caggatatta ataataccgg
    36901 tatcccgctt tccctgaaag agtttatgga attcgatttt aatcctcttt tatccgaagt
    36961 ggccgatctt attaatcatt gctcaaaact ttgggagaaa ataccgatgt tggccaggac
    37021 tcatgggcag cctgcttcac caacatttgt gggaaaggag ttcctggttt ttaaagtgag
    37081 acttgagcag cagcgcagtt tacttgaatc aattccctat tcagcaaaat ttggaggtgc
    37141 aagtggaaac ctgaatgccc acaaggtgac atatcctgat attaactggg atgcatttgc
    37201 ggatacattc gttgaacaac tggggatgaa acgtagtttt cctactaccc aggtagaaca
    37261 ttatgataat ctggccgctc tcttcgataa tgttaagaga atcaatacta tttttatcga
    37321 cctgtgccgg gatttatggc tttatatttc attcggctat ttttttcaaa agattaagga
    37381 tggagagatt ggttcttcag cgatgcctca taaagtgaat cctattgatt tcgaaaatgc
    37441 agaaggaaac ctgggttttg caaatgcaat ttttgaacac ctcagttcga agttaccggt
    37501 atccaggctt caacgtgatt taactgattc tactgttttg agaaatatag gagttccttt
    37561 ttcacatacc gtggttgctt accgatcact gcttaaaggt ttgggtaaat tgcagttaaa
    37621 caaggaagct attgacagtg atttacaaga taactgggtg gtagttgctg aagcaattca
    37681 aaacatattg aagcgggaag gtcactcaaa accttatgaa attctgaaag accttacgcg
    37741 taaaagtgaa aagatcactg aacaggaagt aaaaaacttt attgaacaac ttaaagtttc
    37801 agacaaggtt aaggaagaac ttaagaaaat cactccattt aattacacag gttaagaatt
    37861 agcctaccct gtctccagtc ttctggcggc actccctggt cttatttttt tcgacaactt
    37921 tccagaaaat actgtgcttt agagtaataa ttaatttgac accgaaattg tgccagcttt
    37981 tcttaaaccg agccttaaag attggattgg gctgcggttg aattacataa atatatcctt
    38041 ttgatcttgt aaattcctcg aagcacaaat tgccatacaa aattccctgg gtgtcagact
    38101 ttaggatctc cagatatttt tttgcttttg ggccttcctt gctgatatca actctcggat
    38161 tttgatcaat tttatttgtt ttaggtggtt tatcctgggc gaaggtaaag gagcatagtc
    38221 cagttagaaa tacacctaga aaaatatatt ctaaatacgc aactcttctc attgcttaaa
    38281 agtacaggat atttgggaat cctgcattta tttctgtcta cgggattaat tgtttttatt
    38341 taatcttccg gcagatacag aatctgattg gttgcaaaat catggagagt tagtcttcta
    38401 aggttataat aggcgcccca aaagttacgc aatgaggtga tataagtacg cttcgcatta
    38461 tctttttctg ttaaggcaag acttaaatca gtaatactga tcttgccaat cagatacctg
    38521 ttttttgaaa tatcatatct acgtagtgaa atttcatccg ctttttgtgt gataacaatt
    38581 tggtctttaa gcatcttgaa ggttctcacc tgggttagta cttcctggtc gaaattctgt
    38641 tcatcctgct gaactgtaaa atcaactaat ttcagatttg cttcagctgt tttttttctg
    38701 gatttctgcc gcccccaatc cacaattggg ataaagaacc caagtcttac ccgttgttga
    38761 tctccagggt tattatagac tcctgggaag tctgtggcct gattgcttaa accaaaaacc
    38821 ccaaacaatg aagcgtctaa tccggattca cctacagccc tggaaacatc tctttccgcc
    38881 tccagttttc ttctgtcaaa cgcgactgct tcctgcctgt tattacgagc ttgctgcaaa
    38941 gctacttgct catccactgc aaattccgga acggtagcgg gcacaataag cacaagattt
    39001 tcatcggtaa ttccaagata tgatctcaac cgtaattgtg aagtttcaaa attcaactct
    39061 gactccgcga ctgcctgtcg ggagttcatc aggttaagtt ccaattgcaa taactcattt
    39121 tcaggaattt taccaagttc atatcggcct tgcgcaattt tgaaaattgt atcattactt
    39181 tccacgttct tcctggcaat atctaatgaa atctgtgcta tcagtaaatc aaaaaataaa
    39241 ttactggcag tttgtgaaat actctccaaa tcttccgcga actttcttct ggattcttca
    39301 taacgaagcg gctcgatccg ccgatcccac cgaagttgat taaactggaa caagggctga
    39361 gaaaatccaa tttccaatgg attgccacca tatacttgag cattggtctc caaattgtca
    39421 aatcgtgaaa tactgctatt gagaaaaacc tgtgtgccgg taggggcaat agcctgactt
    39481 aagcttaacg ttaaatcgga gttcatcctg ttcactgaag gatagctgta agtaccatcc
    39541 ggttgaacca caggtgttac cgagtgattg aagtcaggaa gggttccgtc aagagccaac
    39601 cgtggtgcat aattactttt ataggttcga aattgccaat acctgttttc ccggattgtt
    39661 tcggcaagaa tggccgccgt ggactgttgc tttgccatac gtactacttc ttccaaagtt
    39721 aaaatggttc tttcctgtga ataaaccggt gatgaagaga taatacaaat aatgagaatg
    39781 gataacttat tcatgacgta atgatgttat tggatcctga ttagcggctc gccgggccgg
    39841 ggctaatccg aaaattaaac caacggtcgc ggctacacca aatgataata tgatagatga
    39901 aaacgtaatt attgttggga tctcggcaat ccttgacact ccaattgcaa tgcccacgcc
    39961 caacataact cctattaaac ctccggtaat acttatcata atcgcttcaa acaaaaattg
    40021 aagtataata tctttttttt gtgctccgat cgataagcgt aatcctattt cttttattct
    40081 ctccatcacg gaagccagca taatattcat gatacctata ccaccaacca gaagtgaaat
    40141 tcccgcgatt gctcctaaaa caaaattgaa aatacttttg gttcgttgtt gttgtttcaa
    40201 taagagttcc ggaattgtta tttcataatc aacaacttca aaatgtcttc gttggatcat
    40261 cctggccaca atatctgcta ccggatttag caattccgtt tcgccaacct ggatcaccag
    40321 gcgatcaagt tgatggtaat tctcctgggt agtgggttca tcagtggatg aagagcctat
    40381 tatgatcaca ttgccccggc tgattcttgc tcctgcttgc acgccggtgc ttgtaagcaa
    40441 atccctgttt tcgtatctga tcaacatagc ctggataggt atatatacat ccatattata
    40501 gtctcggatg cccaggtttg aaattgcatt ttcgctaatt aatctttctt ccagaacacc
    40561 gataatctcc agccattgcg atccgcactt tatggattta cctatagggt tatccgtagg
    40621 aaagaatttg gtatttattc cttttcctat aatacatacg gcatctcccc gttttaggtg
    40681 ctcatcgtta aacattttcc catccgacaa cacaaaatta gctacttcaa aatattcggg
    40741 ctgcactcca accaatttag cagaccgtct taagccactc tttatgatgt aagtttcaat
    40801 aagaatttca gggctgagtc tttctatccc agggacaatt tttctgatac tttcggcatc
    40861 acgcagtgtt agcccgggag aaaatttctt tttctcctta ccggtagcaa catcttcctc
    40921 tacagctcct tcagattgct caaccacagg agttatcaca atattgttaa cgcctaccag
    40981 cttaatttgc tccagtattt cctgctgagc tccatttcca atggcaagca tagcaattac
    41041 cgccgctact ccgaaaataa tcccgagggc tgtaagtatt gatcgcatct tgttggcaaa
    41101 gaccgcctca agagcaatgt agatatttgc aataaacctt tccgacactt tgctttaatt
    41161 atctatctcc ttgcggacgc gcacctgatg tacttcctgg ggtttgacca ggccgcctaa
    41221 atctgcttgc aggaactacc tgaccgttgg gtaaagttat catgggctcc aattc
//
LOCUS       DASWHX010000189        49997 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_3180785, whole genome shotgun sequence.
ACCESSION   DASWHX010000189 DASWHX010000000
VERSION     DASWHX010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 49997)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49997)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49997
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3180785"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1092)
                     /locus_tag="VGA21_12465"
     CDS             complement(<1..1092)
                     /locus_tag="VGA21_12465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013453821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HEX9651368.1"
                     /translation="MNRVIRKIAILGSGVMGSRIAAHFANIGVQVLLLDITPSKLNDE
                     EIQKGLTLEDPAVRNRIVNSSFESTRKSKPNSFYHKDFVSRVSLGNFEDDLQKISECD
                     WIMEVVVENLEIKKSLFEKVEKHRKPGTIITSNTSGIPIHLMAEGRSGDFRKNFCGTH
                     FFNPPRYLPLLEIIPTPETDSALTDFLMYYGDRFLGKTTVLCKDTPAFIANRVGVYAI
                     LKVVETMKKLNLTIDEIDKLTGPVIGRPKSATFRTSDIVGLDTLIKVTNNLYQALGND
                     ESRETFKLPAEIDVLQNNNWLGDKSGQGFYKKSKDSKGNTQILTLDLESMEYKPKSRV
                     KFDTLEKTKPIENIRERFKILLGGKDKAGE"
     gene            complement(1089..1562)
                     /locus_tag="VGA21_12470"
     CDS             complement(1089..1562)
                     /locus_tag="VGA21_12470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019948451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX9651369.1"
                     /translation="MKKKSMKREETIDFNIKASWHAIYRMYNQEAIKYEFTITMAFVL
                     LNIDRENGTPAMKIAPLMGMEARSLTRVLKSMEKKGLICRKKHENDGRSVRIFLTTLG
                     NEKQLIAKEKVKIFNAVIRDSIPEEKITNMIETLTEINKLIEQNNIFEYVIANQR"
     gene            1699..2031
                     /locus_tag="VGA21_12475"
     CDS             1699..2031
                     /locus_tag="VGA21_12475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002700055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEX9651370.1"
                     /translation="MHSSELLKGNLQTIILKVLSDQKRMYGYEIVQKVKQSSIETIVL
                     TEGSLYPMLHKMEADGLLKIELEYVGKRIRKYYSLTETGASQVEIKLKEFAAFVKTMS
                     AVLQLKLT"
     gene            2031..4715
                     /locus_tag="VGA21_12480"
     CDS             2031..4715
                     /locus_tag="VGA21_12480"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9651371.1"
                     /translation="MKILPEKYLHYIRKDLESKGLIHEGLEDEIVDHISTLVENRMGQ
                     GKRFMEAYQDALKEFGGVGRIRQVQKETIQFENYKTKIMLQNYLKIAYRNIVKHRFYS
                     MINVLGLAIGIACCILILLYVEDETGYDKHLANSHRIYRVASDHLFHGNEFRAAVTPA
                     PLASALINDFPEVEASARLRNGGSIVFRKGESAIKEPSLAYADSTFFNVFPFNFLEGN
                     PETALVKPNSIVINQTTAQKYFGNSNAAGKTLLTSEGTELLVTAVIEDIPVNTHFQIP
                     IFITMANWSRANNDNWMSNNFHTYLLLREGYKSEELEAKFPGMVEKYVGPQIRQFTGV
                     SLEEAEAGGSRAKFFLQPVTSIHLESDLAVELGVNGDITYMYIFSSIAIFILIIACIN
                     FMNMSTARSAGRAKEVGIRKVLGSYRSHLIRQFLTESVILTLLAGILAFAFAVLALPY
                     FNWVAGKSISMPFSDVTFVLVTLFGIITVGVLSGFYPSFILSAFRPAQGMKGVFNAGR
                     RSTNIRSILVVFQFVISVVLILGTLVIYNQLNYIQHKNLGFEKDQVLVIRNAYLLDNN
                     LEPYKNSLVSESLVSGATISSYLPAGGPRSDNTYFPKGNPELESGVNMQVWSVDHDYL
                     KTFKMDIAAGRDFSIDFPSDRSALILNESAAAGFGFENPIGEIISEYYWDPTTGAINQ
                     DSMIDHQVIGVVRDFHFSSLRNNISPLCLRLNRSAGSISVRFNAASASEIIDLARTKW
                     FSLAPGQPFEYFFLDDQFNSIYSSEKRTGKIFATFAGLAIFVACLGLFILSAYTTERR
                     TKEIGVRKVLGASQKSIIFLLSKEFGKLIVAAFIIAIPLGIYGMNAWLSQFAYKANPG
                     IFTFLSAGLVALLVAFVTMSYQSFRAARTNPADSLRNE"
     gene            4816..7239
                     /locus_tag="VGA21_12485"
     CDS             4816..7239
                     /locus_tag="VGA21_12485"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9651372.1"
                     /translation="MWTNYFKVAFRNIKRQKFYSFTNIFGLSVGMASTILILLYVADE
                     LSYDKFHKDSDLIYRVGIFGRLAGQDFNGATSPAPMASTLVDEIPQINAAVRLNIWTK
                     VITRYEELAFTEEKLLLADSNFFSFFSFKLIQGNKDEVLKGPNKLVITESAAKKYFGY
                     SGPGDQTPIGKLLGIGNGSRTCEVVGIAEDPPANSHFHFTLIMSMDSWDASRQTQWTS
                     NSFYTYYKTIPAAGDMNPTFESLVDKYVGPEVKQYLGIDMNEFRNQGGAYSYILEPLT
                     EIHLNSRLDNQLEPGGRKEYLYVFAAIAAFIILIACINFMNLTTARSADRAKEVGIRK
                     TIGALRYRLMGQFFSESILFTLISAVISVLIIIVALPYFNTLSGKEIGLDVLMNSKYL
                     LGILILIALVGIGAGSYPAIYLTSFIPAEVLKGRIRAGLKSGGIRSFLVVLQFSISIG
                     LIISTLLVYQQLDLMQKKNLGFKKDNILVVDNAPKLGNNKKVFKDLLKQNPVIENVSF
                     SNMTPPHIDNNSVFRPLGENAEDNLFFYFRVDEDYDELMALDLVQGRFFADDIASDSN
                     AVILNEAAMKQLGWVDYEGKQLLSFNRSESGDIINVIGVIKDFNFESLRNEVKPLLFF
                     NAPDWGLVSVRLKEGTDFQSDINFIEEKWKEFTGNSPFDFVFLDEDFDALFRAEQRMG
                     NIFLLFTVLAIAIACLGLFGLASFTSEQRAKEISIRKVMGASMSQVVVLLSGNFTKLV
                     LISFVIATPAAWYGMNKWLQGFAYRVEISILTIAIGGLVALVIAWLTISMQALKAAKS
                     SPVNSLRAE"
     gene            complement(7240..7926)
                     /locus_tag="VGA21_12490"
     CDS             complement(7240..7926)
                     /locus_tag="VGA21_12490"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEX9651373.1"
                     /translation="MEIIKCMIVDDEPLGRDVLEKFILQTPGLKLSAVCKNGFEALQF
                     IHETEIDLIFLDINMPKLSGLSFVKTMTKSPKVIFVTAYPEYAVEGFEVNAVDYLVKP
                     VSFERFIKSVNKVKELMQLNHAIDADSIFVKVEKKLFKLRFSDIFYIEAYGDYLKLHT
                     GKNILIATETMKNIEDKLPVAIFQRIHKSFIINLTKMEFIEANQVRIKERFLPVGATY
                     KEEFEKRLKK"
     gene            complement(7914..8954)
                     /locus_tag="VGA21_12495"
     CDS             complement(7914..8954)
                     /locus_tag="VGA21_12495"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX9651374.1"
                     /translation="MKSLASDRNKIQKILLHTFFWILSFYILLRIFVRGNEIESIDII
                     YTFLFQCTIAPFAYANLLWLKPLLDSGKWYLYGFILMLIVSAAAGFNYFFFTVGVDFV
                     LPGYYFISYYDWIEIIQFVFGHLFILTLLQISLEWFELNRERQRISELEKEKINFEMK
                     ALLGQINPHFLFNTLNNLYSMVLNGSQQSGEYILKLADVLRYVIYQSRQEAILLEDEI
                     RLLTDYILLQELRTDYPNAEFNVSGETGTWRIPPMIFLPLLENSFKHGIKGEKESQKI
                     IINLQVNEREIFFKIQNEREEKETIRDKQPGGIGMENIKRRLELVFPENHEFNIEERE
                     NLFFTNLKIWKS"
     gene            complement(8954..9562)
                     /locus_tag="VGA21_12500"
     CDS             complement(8954..9562)
                     /locus_tag="VGA21_12500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651375.1"
                     /translation="MKKFLIVLLIITSCVPSIHPLYHERDLIEDQRLFGNWRNAGEGE
                     FWDGRESWKFEKLPPKQGFLGSKTPDPLSGYLLTITEDSIAAEFVARLLELDNQLFLD
                     IFPKSEYGDIKGIENNWLQMHLFPVHTFARISFENKAMIIEPFDPDFIIDLLEQKKIR
                     ISHEKTGNNFILTASTDELQKFVMKFGREKEAYIDPGLFTRM"
     gene            complement(9668..10783)
                     /locus_tag="VGA21_12505"
     CDS             complement(9668..10783)
                     /locus_tag="VGA21_12505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008197693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HEX9651376.1"
                     /translation="MKRLILYHLIIFFSSLAQLSAQNYYVYVTAESEDEVSLIKFDGK
                     KAEVIKTIPIGIWPADIEGPHGLTMSLDKKYWFVSVAHGNPFGTLYKYSTETNELVGK
                     TDLGLFPASMEVSKATGLLYCVNFNLHGDMVPSTVSVVDPETMTELKQIETGVMPHGS
                     RISSNGLLQYSVAMMSGELFEINTVNLEVSRMLVLDEKEMTMSGGMDHSAMDYETGAA
                     KKMVHSKTKPTWAMPHPTLQVVYVAGNGSDELLEIDTKLWKVTHRLKSGKAPYNVDVT
                     PDGLKVVVTYKGEGATGVWDVATRKELAKISNTRKVSHGIAISPDSKYAFISVEGIGG
                     EPGTMDVIDLTTFKKVSYAELGKQAGGIIFWKMEQLD"
     gene            complement(10792..12768)
                     /locus_tag="VGA21_12510"
     CDS             complement(10792..12768)
                     /locus_tag="VGA21_12510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008197691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEX9651377.1"
                     /translation="MKRIATGLLCCWIFCSANAQSKVSIKVDKIPLTIDVGQSLPLNA
                     RVVDENNRTIEERTLRYFSRKRRAVSVDSTGLVTGILPGEYSLIIISPASDGNVARVD
                     IPVIVNYPPISRIEILDFPSSIYENTNQVIRTKVTDAANLERINIDLEYSSRNPEIAS
                     VDMFGNVLANKKGKTTISVKKEAIATEVTVMVVENPTAKVALKTTVSEARAGDVIKLE
                     ATTTDKSGKKLTDIPVYFSFEGRAFDVSSTASGLIQQDGRFVAEEPGMYTLIAKSGNF
                     ADQKTIRIIDRGVQRKVERVGKGSVANKHTSDFWIWEGLDGRDYGVTGTWGADGTAYF
                     WDVTDPSDIVKIDSIQVDARTVNDVKVSEDGKICIISREGASNRKNGIVILDVSNPRD
                     VSIISTFTENLTGGVHNLFISKNHVYALSAGQKYYSINIEDPKNPKIAGKFELDTPGH
                     SIHDVWIEDGIAYSSNWGDGVQLVDVGNGIAGGTPDNPVQFASYAYPSGANHATFPYR
                     SKSTGKFYVFAGDEIFPYGLNLKGANVAGGFIHIIDFTDLENPVEVARFEVPGAGSHN
                     LWIENDMLYVAFYNGGVRVVDISGELMGDLFNQGREVGWLIPGDPDGFVPNAPFTWGA
                     QPYKGHIYISDWNSGLWAVRLEPEKPKETQIQSK"
     gene            complement(12848..14710)
                     /locus_tag="VGA21_12515"
     CDS             complement(12848..14710)
                     /locus_tag="VGA21_12515"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9651378.1"
                     /translation="MGMNIVEKSDMKFQLAFLLLFLAFNTFSQNTNEIELANEYYAKA
                     ELEKAREMYEQLARNTQNIPYIHQNYFNVLVELGDFSGAESYLDKVIKRYPDNLYYQL
                     DIGTLYVRQGDSEKANTYFRKLISKIKDDNYKVRITAQYFINKQLMDWAVITYNEGRR
                     AMNNDLLFSLEMANIYRMQNQKDKMVGEYLNYVVQNPSNLNYVKNTLQNLLSEPDELE
                     SLETLLYERVQSEPGNELYAELLIWVNLQQKNFYGAFIQARALDKRAKSDGRRVLDVG
                     LIAMRNEDYEEAIKIFDYIIKEYPGSFNFIVSRMYRIKSREEIVKKFYPVNIDDIKAL
                     VNDYDAFVNDIGINTTTLEAMRNQAQLYAFYLDEKEEAIRILNEIIKSSAANQELVAE
                     SKLTLGDIYILTGETWESTLLYSQVEKSMKESKSGYEAKLRNARLSYYKGEFDLARDH
                     LNILKTATTREIANDAMDLSLLIVENTGLDADTTNAAMKKFAAIELLLFQNKAESAIQ
                     QIDSMLVHFSNHTLADELYMQKAKILMKAGQFDQTITLLEKIVEEYGYDVLGDNAYYT
                     LGKVYEEQLKNKEKAMEIYQDFLVRYPGSIFATDARKRFRKLRGDFSASEKIIN"
     gene            complement(15042..16682)
                     /locus_tag="VGA21_12520"
     CDS             complement(15042..16682)
                     /locus_tag="VGA21_12520"
                     /inference="COORDINATES: protein motif:HMM:NF024603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEX9651379.1"
                     /translation="MKIKVHLTIVIAVIAWGCANQTQPTGGPKDENPPILLSSSPENG
                     ALNFKGQVIELQFNEMIKEANLQSQLIITPSVNGKFNSKINRNKLQITFEKAFNDSTT
                     YTLNFRESIKDVTESNSVENLVLVFSTGSYLDSLGISGNVITLLTNEPAANYTVAIYS
                     ANDTLNIFNSAPLYATQTNTDGDFQFLNIKSDYYTIYTWKDKNNDLKCQSESEPFAFL
                     NEILELDSTRSDIFLKPFPFNIKPLTITSARQAGQYYEVRFSKRTTSYFIQLYNQQDS
                     LYHHYIDENKAIRIYNTSPSNPEIQFSLTVLDSAEQKITDTIRFSFLPNSRPKEPFSA
                     SITMANLKTINSEFIATIEFTKPIKKFIDTLWTINLDSVSIPLPDFGPSRVFQPNPLQ
                     LQIITTLPDSLTLMKNLSFKMNINSLQSIESDTNTLMTKPIAILNPDNFGTIELQFPG
                     DSFPFNVHLLSAKGVLQKVYPGNSRVLMEYLGADQYKIRVLIDANADGVWRPGNFRNR
                     IMPEPVIFFYDQESKTDIITLKKNWVLGPFLIPLSVDN"
     gene            complement(16679..17560)
                     /locus_tag="VGA21_12525"
     CDS             complement(16679..17560)
                     /locus_tag="VGA21_12525"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX9651380.1"
                     /translation="MFEKILECPVCNGSKFKNQIICQDFLVSGESFVLTDCESCGLRL
                     TNPRPNQESIGRYYESDHYDPHSGKTSNIFNLIYKTVRSINLTWKKNLINQYSPHGRL
                     LDFGTGTGEFLLKMKSNAWEVEGTELHGRPREFGNQKYNLDIKENLSQLTHNQVFTAI
                     TLWHVLEHIYDINSLLQQLASLLSKDGKLIIATPNILSYDAVKYGQDWAGYDVPRHLN
                     HFKPKTMKKLLKRNGLTLQNIIPLKMDAYYVSILSEKNLKHSLPFLKGMIAGFRSNSW
                     AAKNSDNYSSLVYIVRK"
     gene            complement(17562..19424)
                     /gene="mnmG"
                     /locus_tag="VGA21_12530"
     CDS             complement(17562..19424)
                     /gene="mnmG"
                     /locus_tag="VGA21_12530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008628287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEX9651381.1"
                     /translation="MFREYDVIVVGAGHAGCEAAAAAATMGSKVLLATMNMTTIAQMS
                     CNPAMGGIAKGQIVREIDALGGLSGIISDKSMIQFRMLNKSKGPAMWSPRTQNDRLRF
                     SEEWRLALEKIENLDFWQEMVKEIVIEKGNAIGIRTAMGIEIRAKSVILTNGTFLNGI
                     IHIGEKQFGGGRTGEKAATGLTEQLVSFGFQSGRMKTGTPPRIDGRSLNYSKMEEQKG
                     DETPGKFSYMDTQPLSVQKSCHITYTNPVVHEILQSGFDKSPMFSGRIEGIGPRYCPS
                     IEDKINRFAERDRHQIFVEPEGWNTVEVYVNGFSTSLPEDVQYAALQQIPGFEKAKMF
                     RPGYAIEYDYFPPTQLNLSLETKGIQNLYFAGQINGTTGYEEAASQGLIAGINANNKV
                     HNKEPFLLRRSDAYIGVLIDDLINKGTDEPYRMFTSRAEFRLLLRQDNADLRLTESGH
                     RLGLASDLRLERVLDKKNETTELLATLSKLKVTPTEINGTLTSLITATIKEKVSVENL
                     LRRPEISLQGLLKNDHKLESRLSKFSSEGKEQAEILIKYDSYLKKEQEMVDKMGSLEH
                     YIINDGFNYDRIKGLSAEGREKLKFINPKSLGQASRISGVSPADISIIMVYLGK"
     gene            complement(19500..19919)
                     /gene="ybeY"
                     /locus_tag="VGA21_12535"
     CDS             complement(19500..19919)
                     /gene="ybeY"
                     /locus_tag="VGA21_12535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009580807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEX9651382.1"
                     /translation="MNGSVSFHTQDIEFEVPNPQKTRKWILGIIRNEKRELAGIHYLF
                     CSDDFLLSLNQKFLMHNAYTDILTFDYSKKGVLSADIYISIDRIKENSTKFKKTFEQE
                     LRRVMIHGVLHLLGYSDKTTGEKSAMRKREEACLSLY"
     gene            complement(19922..20323)
                     /locus_tag="VGA21_12540"
     CDS             complement(19922..20323)
                     /locus_tag="VGA21_12540"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011586840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9651383.1"
                     /translation="MNSIKIEIPSLSENIRIIESFIDNAKDRFNLNDDIYGNIMVAVT
                     EGVNNAIQHGNKNDKKKNVSITLQLDESQIVFRIMDQGSGFDFRNLPDPTSPENLSKP
                     GGRGIFLMRHLCDGVNFIDDGRTVELVFYIN"
     gene            complement(20342..23764)
                     /locus_tag="VGA21_12545"
     CDS             complement(20342..23764)
                     /locus_tag="VGA21_12545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017730993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4175 family protein"
                     /protein_id="HEX9651384.1"
                     /translation="MSESVNGLAKKIKEYKKKFYLNLILKGSIITASIILTFFLAANL
                     VEYVGRFSSIIRGLIFFAFVISSIGLLVYFILIPLFKSNDPENGLRDEDAARMIGGFF
                     PEIRDKLLNIIQLQKMNNGKNQLVSASINQKSGNLRNFTFATAINLGHNRKYLRYLVP
                     PAIIALILAVVLPNLFSTSTQRIVQFSREFIPAAPFQFVIDSQKLLAFKNEDFTIELT
                     LTGEAFPENVYLVNNGRKMKMNRLNASSFEFTFQKIQAEKSFYFESAGFTSFEFELKI
                     ASRPNLKSFDVHINYPSYLGKDPERFSNVGNLQIPEGSIVTWQFQTLEAMELQVDFKD
                     SKNDILFQNLDNQFVEAKKQFFKSEEYEIKLRNKFSENLQKINYSVSVIPDQYPEINL
                     NIFQDTVLYSFLILGGYLEDDYGLSRLGLFYEIERNNNKSDWQSIPIPLVSHQNNQSF
                     YYEWTLGDMNIKEGEKINYYLQVWDNDGVNGAKSTKTGTYTFMIPTRDEIKKEIEAAT
                     SKSEDKIEETLKQTEDLRKKLEEAENRLRGKKDMDWQDENLLNEILQKKHELEEALKK
                     LSEENKANDLKRQRFDEENARIREKVEQLQKLMDDLLDEETRKLYEELQKLLEEKEAS
                     EEMQKLINRINQKENNLEKELERTLELFKKMKFEYKLNETIHELEELAEEQEELARKT
                     GEDKVGEEEMEQKSEEGKQEEGSQQEENSVNQEAEEKDSQNSGENEGENENLTGQQEE
                     LNTDFEQIKENIEEIQDLNQNLKNPQSMQDFNDEQQQIQENQQKSLENLKQNQNKKAQ
                     DSQKKAAQQMKQLSQKLQEMQGGMEMETMTENMENLRDILHNLLQLSFDQEKLMNDFR
                     EINQSDPRFVEFSQQQLKIKDESKIVKDSLLSLASRVFQIQSFVTREVSEMNRYIDEA
                     MEALRDRGRNVIPVTTGKQQFAMTSMNNLALMLDDVLQQMQQAMSEAMGNPQKGKGNQ
                     RMPGMTELQQQLNQQIQQLKESGKSGRELSEELAKLAAEQERIRRALQEAEEKLNQGQ
                     GQGELQELMNQMEETELDLVNKHLTEETIERQNEILTRLLDAENSIRERDLDDEREAE
                     TAKSYENLVPKAFEDYFKLKEKEVELLKTVPLKLFPYYKKEVNEYFKRIGEN"
     gene            complement(23889..24260)
                     /locus_tag="VGA21_12550"
     CDS             complement(23889..24260)
                     /locus_tag="VGA21_12550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009197093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651385.1"
                     /translation="MLKKDHENEKRWQRLLKLLEDRIGKKPADLNSVLFLIGLQELGK
                     GPKSFSKEQKQDIMHIAICKVLSLSGFYQLEGHDEEGWPHWKLVKKLPAFDLLEQEKL
                     LKMHVIEYFESEVWPNERMME"
     gene            complement(24253..24600)
                     /locus_tag="VGA21_12555"
     CDS             complement(24253..24600)
                     /locus_tag="VGA21_12555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018472351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX9651386.1"
                     /translation="MSIVSDFNDYRHRMNDAILDSDNKILKRIFNLDTNAFQEGDLDV
                     KTKEMIGLACSMTLRCDDCVRYHLGKCFELSVSDKAVFEVFAIANLIGGTIVIPHLRR
                     AVEYWEELKKSNA"
     gene            24648..25322
                     /locus_tag="VGA21_12560"
     CDS             24648..25322
                     /locus_tag="VGA21_12560"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEX9651387.1"
                     /translation="MIRDFVNLLFPQWCAGCMGHLVSGEESVCTFCRSELKISSTYKE
                     KPNGIVSKFYGKIPVEHAFTMFQFSRKGVIQNLLHRIKYGGYYDMGVWLGNLLGAEIK
                     HEPWISDLNIIVPIPLHKSKLQLRGYNQSEAFARGLSGAIQVPIDEVMVRKQHTQTQT
                     RKSRMERWVNVSEIFELTRGKFVEGKNVLLVDDVITSGATLEAAGSVLIKKGVNKIYI
                     ATIANA"
     gene            complement(25370..26557)
                     /gene="gldJ"
                     /locus_tag="VGA21_12565"
     CDS             complement(25370..26557)
                     /gene="gldJ"
                     /locus_tag="VGA21_12565"
                     /inference="COORDINATES: protein motif:HMM:TIGR03530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility lipoprotein GldJ"
                     /protein_id="HEX9651388.1"
                     /translation="MSVVLLGSCQFIQNIFGGGSRPTSTKPGKLSTTTGLAYNDEQGG
                     FQVSPFEGQPDGPNLVFIEGGRTVLGSSEQDIMNSRDNIERTVTVASFYMDETEIANI
                     HWLEYLYYIQQDSTREYYLAALPDTTVWSRELAYNDPYVDHYFRYPGFRYFPVVGVSW
                     IQAIDYTEWRTAAVNQSLAEQAGIDYTAYPQGRIPLETGVVLPNYRLPTEAEWEYAAM
                     ALIGTQWLDENQTHKRLYPWDGHALRNPYGRDMGYFLGNFKRGRGDYAGIAGKLNDGA
                     MITSYIYEYPPNDYGLYNMAGNVNEWVWDVYRPLSFEDVDDLNPARRNGWLDEENQYD
                     TGYDDNDWQSLISNKSRVYKGGSWKDVAYWLSPGTRRYLEEDSATSTIGFRCAMIRAG
                     SNY"
     gene            26916..27998
                     /locus_tag="VGA21_12570"
     CDS             26916..27998
                     /locus_tag="VGA21_12570"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX9651389.1"
                     /translation="MEESIRQRKKARLYWILNISGWCIFLVVYSWIAVTMYLFHWKIL
                     LAYFNSTIIGFSLTHFLRLFIKKKEWIKLPIRRLALYTVVASLIMGAIWALITYPINY
                     SLLGLNELEGLDKFRNENIGLVNTILTVHFQFSLLMFIWCLLYFGINFFYHFRESEVE
                     KWKLNAMVREAELQALKSQINPHFIFNSLNNIRSLVLEDPEKARSMITNLSGLLRYSI
                     QFSRKEKVKLEDEIEVVKNYLKLESIQYEDRLRYKLEITEEALDFKIPPMIIQILVEN
                     AIKHGIAALPEGGEVNIRTHLFEDDLVVEVINSGRYKTEIAGTGIGLQNASERLRMLF
                     GKLSTLTVENLDDNRVIAKFSVPITI"
     gene            28000..28716
                     /locus_tag="VGA21_12575"
     CDS             28000..28716
                     /locus_tag="VGA21_12575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018619493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEX9651390.1"
                     /translation="MKAIIIDDSRLARNELKRLLTDFDFLQIIGEASNAIEAKEKIES
                     QKPDVIFLDIQMPGKDGFELLEELEVIPQVIFTTAYDQYAIKAFEVNALDYLQKPIEK
                     DRLSAAINRVVERQNDQAAKGDSDYLKPTDQVFVKDGERCWFVLLSEVPFFEVDGNYT
                     KIFFKDQRPMIPRTLNYLESRLDPKFFFRANRRQIINLSWIEKIEPWFSGSIRIYLKG
                     SKEIDVSRRQTQRFKELTGI"
     gene            28876..29118
                     /locus_tag="VGA21_12580"
     CDS             28876..29118
                     /locus_tag="VGA21_12580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651391.1"
                     /translation="MSFKEKCSEITFHGKLLATKKLGNHQVFLFWLKSFYVELWFNNR
                     KGEIHGINSFESVKGLDFYLDDFSIQEIRKIVTDKN"
     gene            complement(29115..32456)
                     /gene="mfd"
                     /locus_tag="VGA21_12585"
     CDS             complement(29115..32456)
                     /gene="mfd"
                     /locus_tag="VGA21_12585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009578620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEX9651392.1"
                     /translation="MTREDLIRSYSEDGIIKTLAENFHTEKIIQLKGLSGSISAVILA
                     SLFSTDKSTSLVVLADKEDAAYFFNDVREIIGNEKVLFFPASYKRPYQYEEIENANVL
                     LRSEVLTWLNNDKSEPTVIITYSEALGEKVVNKRSLSKNTYPISVGDRIDQEFLISVL
                     INYDFEKADFVYEAGQFAVRGGIVDIFSFSNEIPYRVEFFGDSVESIRTFNPESQLSI
                     DKLNSLSLIPNVQTKLLHEERQPFLHFLPANSSIWFDDLKMTLDIIESNFAKCSVEFN
                     SILESAGNTQVVLSPETLFENKESFLKCLSELRVVEFGSRFHFRRSFLLQFNTHPQPS
                     FNKNFELMARELEANQRAGIRNIIAAESPKQLERLMTIFEEINPDLKAEELNISIHKG
                     FIDKDLGQAIYTDHEVFERFHRYRLRDQFTKSRALTLKELQNLHPGDYVTHIDYGIGR
                     FVGMETVEMNGRKQESIRLIYRDDDLLFLSVHSLHKISKYSGKEGVPPVMSKLGSAEW
                     DNKKKRIKKKVIDIASDLIKLYAKRKQSEGFAFSADSFLQAELESSFIYEDTPDQAKA
                     TEDVKVDMQEAHPMDRLVCGDVGFGKTEVAIRAAFKAVCDGKQAAVLVPTTILAMQHY
                     RTFTERLANFPVGIEYVNRFKPARKVKEILQDVDSGKINILIGTHRIVNKDVRFKDLG
                     LLVIDEEQKFGVKVKERLKEIKVNVDVLTLTATPIPRTLHFSLMGARDLSIISTPPAN
                     RQPVTTELHEFNETVIRDAISFELQRGGQVFFVHNRISDIDEVANIVLRLVPDARIGV
                     AHGQMVGDKLEKTMLRFIEGYYDILVSTNIIESGLDIPNANTIIISHAHMFGMSDLHQ
                     MRGRVGRSNRKAFCYLLTPPSFMLSSDARKRLTALEEFSELGDGFKVAMRDLDIRGAG
                     NLLGSEQSGFINDLGFETYHKLLDETIQELKETEFKDLFIKPNQNLQYLVQDCIIETD
                     LEVLIPDFYVENISERLSLYSKLDNIKTEDELKAFTDSISDRFGKLPDSVGELIETVK
                     LRWLGESLGFEKIVLKNSELKCHFISPNKETYFQSDTFGKIIDFVQSHSSKCKLLDRK
                     NRLVLTITDIPSVHAAIDFLIKIKI"
     gene            32564..33742
                     /locus_tag="VGA21_12590"
     CDS             32564..33742
                     /locus_tag="VGA21_12590"
                     /inference="COORDINATES: protein motif:HMM:NF018452.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1214 domain-containing protein"
                     /protein_id="HEX9651393.1"
                     /translation="MKKLAFGALNLYRKLKLFRLKLKGMTIEDEHEERIVSGKAWEEF
                     CENLKPAGAALLYGDSPKDPFSQAEGYRYLTRLVRASLEAFVEFSDPEYPVLRRMVHE
                     TIKMGADNPDNYYQNTKINGEYEYRIYGMRGTVHYLGFGTQRGDYGKTGNMEPSGYLE
                     GSELEINPDGTFEIHVSCEKKEGNWLPLTKDSSILMVRQTFLDRKSEVLADLQIERAG
                     GSKKPSMVTPKTIDDGLNAAAAFVSGASLLFSRWSDGFKKNKNKLPRFDQAKSNAAGG
                     DNNIAYYHSYWELADDEALVIETTPPECEHWNFQLNNYWMESLDYRYYTIHINKQSAV
                     YNTDGSVRIIVAHKDPGLPNWINTVGHNLGTMCFRWVKAKENPEPETKVVKFDQVVKL
                     "
     gene            33747..34976
                     /locus_tag="VGA21_12595"
     CDS             33747..34976
                     /locus_tag="VGA21_12595"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEX9651394.1"
                     /translation="MSEDVGLKFEKNYPVPIRIWNFFGKTGLQRKIKTDDLLKKAKRN
                     TRLEDFGNEFFMEPLEVLVKSINEEAELHAFGRYVTKARLVNLLSNRLRAEDYLKRNP
                     QINDIELLPIWWISGLQRTGTTFLHRLLATVPGTATIKSWEALNPVPLEKDFQNKKRI
                     AMAQTSEKGLRYIAPDFFAIHPVEHLSPEEEILLLDISFMSTVPEATLRVPTYAKWLE
                     KQDQTPAYDYMQKLIRIIQFQKGVKKRWVLKSPHHLEYLDTLTNVFPGTNFIFTHRDP
                     LQTIASFCSMIYFSRRIFSSNVDEMEIGHHWLRKIKLMLEKSMKFRAMNKDVECADIS
                     YQNLIKDPFGQVKIILDRYNNTVTETDRRAIQQSVKQNKRDKFGLHNYELQDFGLTGN
                     ALEVEFLEYKTKYSQYL"
     gene            34978..35955
                     /locus_tag="VGA21_12600"
     CDS             34978..35955
                     /locus_tag="VGA21_12600"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9651395.1"
                     /translation="MADYQSPVTATLTGILNAFKKQKRIGDLKETDRVDGKTCLITGA
                     NSGLGFALAVELAKRGAKVLMACRSGIPDAGEKVKKYSTSEKVEMFKVDLSDLTSVDA
                     LVNELKSGMIKIDILICNAATVPSKARKTPQGFETMFVVNYLAKFVLLNRIRKENLLS
                     SDNTPRIIIISSESHRSPKNLEFESFGNYEDFAMSKVVALYGYYKLALTTLAREFSRR
                     TNDKKLNVSVHVICPGAINSNIAREAPGYTKPLIKFIFALFFQKPEKAAKPVSYLACS
                     KEIEGRNDIYLHMYTEKLPDAKTMDTTNGKALWDRSNFLLKSVNYGRTF"
     gene            35984..36778
                     /locus_tag="VGA21_12605"
     CDS             35984..36778
                     /locus_tag="VGA21_12605"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9651396.1"
                     /translation="MYYLRMAGVFVIIYVAICLIYYFIQELLIFHPKKLQQDFEFSYG
                     DKAEEIFLENGSATIHGLYFPVENSKGLILYFHGNSGALDGWGYLGNKFNSIEYSVFV
                     SDYRGYGKSTGRLSEKNLYSDAQVIYDYFTGRCEDERIIIMGRSLGSAIAIELALQNS
                     YEKLILESPFHSLTGMIKMFFNYLPHDLLLQYKFDSQSKIDKIPEPILIFHGTNDEVV
                     PIESARKLESRIQSKYHKFVEIEGGGHNNLSNFDVYNEELIKFLDK"
     gene            36775..37182
                     /locus_tag="VGA21_12610"
     CDS             36775..37182
                     /locus_tag="VGA21_12610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651397.1"
                     /translation="MNFYKLIKAAGILFLLSHPTIAQDPQNMKVENQVLDCGKMKTSF
                     ADIKEAKETLEKTVFAFTQELKTTKRNGIKEVYYYSCDNETGFLLIKEDDREIVYRDY
                     PRFQWKLLIEATDLDGFYDKNVRGKYMLVVRDQ"
     gene            complement(37248..38009)
                     /locus_tag="VGA21_12615"
     CDS             complement(37248..38009)
                     /locus_tag="VGA21_12615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017733845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1/P1 nuclease"
                     /protein_id="HEX9651398.1"
                     /translation="MKKVFAGLLVMCFTQVIGWGVTGHRTVGQIAENHLTKKAKNEID
                     RILDGKSLAYVSNWMDDIRSDSLYDHTHDWHWVTLPDEVSYAEVEKNPDGDIIEAITR
                     LIRELKTHTLDKKTESEHVKMLIHLVGDIHQPLHVGNGEDQGGNDARVKYFGTSTNLH
                     NVWDSQMIDGKNFSYTELANELDKVTPQEVAVLQSASVLDWANESRELRPLVYDLPDH
                     GSLSYEYVYRKWHVVERRLLESGVRLAGVLNDIYR"
     gene            complement(38014..38709)
                     /locus_tag="VGA21_12620"
     CDS             complement(38014..38709)
                     /locus_tag="VGA21_12620"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9651399.1"
                     /translation="MSKPDLLLLHGALGSSSQVAPLSLFLENEFSPGLMDFSGHANPS
                     LFIKDFSFDVFVEDIYNWAGRMSLSKVNLFGYSMGGYAALLFAHRYPDKVNKIFTLGT
                     KLLWTEEESQKESAFLNPGRMEEKAPQFANRLKQMHGLTHWASLVNKTSAFIESMGTN
                     APLTMEIYQQLKCRVLLAVGDKDTMAKVDDTVKVYGWLQNGELLVIPNTSHSIEKVDM
                     ESLANEIKRFMKS"
     gene            38749..39456
                     /locus_tag="VGA21_12625"
     CDS             38749..39456
                     /locus_tag="VGA21_12625"
                     /inference="COORDINATES: protein motif:HMM:NF014626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HEX9651400.1"
                     /translation="MSGRYSIVSSPKSIETHFKAKFEYAFEANANAHPMQMLPVISSL
                     HPDKIVPYYWGLIPYWAKSSDIGQKNFNAWAEGIHKNSSFKIPLERRRCLVPANGYIE
                     WRNFSGIKSPYFVYSPGNPLIALAGLFDTWMNEATEEITRSFTIITTKSNNRLRVISR
                     RMPVILRESNYQKWLDVSKDYRQAMSLLRPIPDKLTNAYHISLSINNPVHNYPELLEP
                     QQDPLAKDNLDIKMFFV"
     gene            complement(39453..40004)
                     /locus_tag="VGA21_12630"
     CDS             complement(39453..40004)
                     /locus_tag="VGA21_12630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017730570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651401.1"
                     /translation="MKKISNAFLLLIFCATISAAQVGDSFPELTAETVENKKVTIPDD
                     TKGKFTLVGLAYSKKSEEDLNTWFTPVYNKFVRSADDAGLFADFTYDINVYFVPMFTG
                     VKAAAQGTAKRQALKELDESLYPHMLFYKGELKTYKEALDFEHKDVPYFFVLDKDGKI
                     IYATSGAFSDKKLGEIEELIDDF"
     gene            complement(40044..41426)
                     /locus_tag="VGA21_12635"
     CDS             complement(40044..41426)
                     /locus_tag="VGA21_12635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020533645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX9651402.1"
                     /translation="MKRIGPYLVLLLLLSCNPPGESQNENLAPVTAFTNVNVIPMDEN
                     HVLENQTVLISEGKITAIGDATSIKVPEQATMIDGTGKFLIPGLAEMHAHIPGTNASP
                     EYLENTLFLYLSNGVTTIRGMLGQPSHLELREKAANNEILSPRIFTSGPSLNGNTVKT
                     PEDAQKMVTEQKEAGYDFLKLHPGIQLEVFNELVASANEVGIPFSGHVSVHVGIRKAI
                     EAQYGSVDHLDGYLEGLVPESANVDPTQNGFFGFNFVKYADLSLLDDLSVSTAAKNVW
                     VVPTHALMKRWAGSLSPDEIGAEPEMKYMPRQTLEAWINNVKQFQSNAAYTVENAIAF
                     IELRDKILKSLYNAGVGMLLGSDAPQIFNVPGFSIQHELKAVVDAGIPNYDALKSGTL
                     NPAIFFGKENEFGMVKAGHSADLILLNANPLDDISAISSRIGVMVRGRWMPESEIQQR
                     LAEIAAQNGY"
     gene            complement(41494..41703)
                     /locus_tag="VGA21_12640"
     CDS             complement(41494..41703)
                     /locus_tag="VGA21_12640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008200723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651403.1"
                     /translation="MSIALNTIRTGKKYFFRNFGEEIIFQVTRILSDHDFKLKDLNSL
                     ETYTFSDLIQFGKGQDYELIELERM"
     gene            41735..43297
                     /locus_tag="VGA21_12645"
     CDS             41735..43297
                     /locus_tag="VGA21_12645"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX9651404.1"
                     /translation="MEGKKMVLLALMMTPGFYGNAQEIKIIENHSDLQPHPLAIINSE
                     FREINLSISPDGKYLYFMSLRGGNPWSVEGYSIFRGKSRYDGDIWYSQKEAGQWTDPI
                     CLPPPVNTSSAEDEPNISPDGNSVMYQSWKDSWLTDGGPYYISKLNGDKWENPEGMSG
                     GINQYFKINFRDYEGYATDGASLSPDGNIFMVALAPDYNDSMDIYISRKDNGIWTYLK
                     NTALSTSGDERSIFIAGDNKTVYFASNGYAGFGGMDIFKTILNDDDSFGEIQNIGKPF
                     NSEKDDQGFIVTASGAESYFVRDDDIYYCDLRLAADELKPLPSIIISGVVTDIFGYPL
                     ESEITISKKETGQLKGKSTSNSKTGEYAVSILKEEGVFEKKVKANGHKIQKENFTLVD
                     MGRFEEKTINTVLVEENTLLIHFDFDEVSLSEYDRSLLDSLVAEMYRNRRLRLMMEGH
                     TDEQGTAEYNSSLSRERVEEVARYLREKGIPEGIMKLKAFGETSPRAGLTVVNRDALN
                     RRVEIKVMELKR"
     gene            43374..43733
                     /locus_tag="VGA21_12650"
     CDS             43374..43733
                     /locus_tag="VGA21_12650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009579066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3127 domain-containing protein"
                     /protein_id="HEX9651405.1"
                     /translation="MNIKGKLLEIFDVVQVTDTFKKREFVLEYMENPQYPEFVKFELT
                     QDRCEILNAFKAGEEVEVWFNLRGRKWTDPKGEVKYFNSLQAWRIQQAGAGQQEDSPR
                     KQEEPGWLAGEQDDLPF"
     gene            43747..45117
                     /locus_tag="VGA21_12655"
     CDS             43747..45117
                     /locus_tag="VGA21_12655"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX9651406.1"
                     /translation="MKKWIWSGAIVILITAVAIIARPILLILSGFGAKNLCSCVYNSG
                     REPSSVLKNELGNFPLTLGNFYLDKKDSSATGSVLGFAKRTAVYKNGEGCTLLVDYSK
                     EEFKQRKPMPIELSAVEIDTLPWPFGTQFPDTVIPGVNYSKLSAIMDDAFTEVEGERK
                     KNTRGVVIVYDGQMILERYSEEFDPETPQMGWSMSKSIINALVGILVSQEKLDVYSPA
                     PVPEWSDRADPRHLISIDQLLRMSSGIEWEENYGAPSSATNMLFTSGDMGKYTASLPL
                     EAPPDSLWKYSSGSSNLLSRIIKQITDEDYLSFVDRELFSKLGIKSIVWEKDESGTLV
                     GSSFMWASPRDWAKLGLLFLNDGVWNGERILPEGWVEYSSTPTPKATRGYGAHFWLNR
                     MQERENTQHPDAPADMFSMQGFEDQRVFIIPSRKLVIVRLGQTSDEHFGFNAFLKNIL
                     ECFPDR"
     gene            45148..46557
                     /locus_tag="VGA21_12660"
     CDS             45148..46557
                     /locus_tag="VGA21_12660"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013455232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEX9651407.1"
                     /translation="MFNQLGLYTDFYELTMAQGFFKNGWARKKAGFDYFFRSLPFEGG
                     YLIFAGLSDLLEMLNRYRFSKDDLKYLRSKGFEKPFLNYLKDFRFQGNIYSCREGEIV
                     FPNEIIVRVEGNIIETQLIETLLLNILNFESLIATKASRVCYAAKGRKVIDFGLRRAQ
                     GFGGFHASKAAAIGGVTVTSNVMSANEFELEVSGTQAHSWIEAFEDELTAFRKFAELY
                     PDNCVLLVDTYNTLKSGVPNAIKIANEMKSRGRELKAIRLDSGDLAYLSKKSREMLDK
                     EGFKNVKIIVSNQLDEHIIRSLLAQNAQVDIFGVGTNLITGKEDAALDGVYKLSFFDR
                     LDRLKISDNIEKTLLPGKKKVLRHLDKGGNFYADVIVSDDHTKPSVMIHPFSPEKRLK
                     LTGFAHENLLIPVMKKGKSTLKENHVSRIAEYASKRLSQLPEEHKRFENPHIYKVGVS
                     NNLLKKRNKMIKDIRSKFE"
     gene            46567..47106
                     /locus_tag="VGA21_12665"
     CDS             46567..47106
                     /locus_tag="VGA21_12665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020526174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamidase"
                     /protein_id="HEX9651408.1"
                     /translation="MKALLIVDVQNDFCPGGSLAVPEGDKVVNVINSLMEKFKIVIAS
                     KDWHPEKTVHFEKWPVHCVQNTKGAEFHPNLNTVGIQNIFLKGTGNKDDGYSAFEATN
                     ENLEDFLKSHGIKELYVTGLATDYCVKASALDSSKLGIKTIVVEDAIKGVEVNPGDVA
                     KALTEMKNQNIEFIQSKEI"
     gene            complement(47132..48034)
                     /locus_tag="VGA21_12670"
     CDS             complement(47132..48034)
                     /locus_tag="VGA21_12670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651409.1"
                     /translation="MHFRFLLCFYLLLLFESRAQDFQPLFEPGAEYTLILNDGTTLTG
                     IVMQFSEGVVTLRNDAGDFRVNVKNIRRIKGGPEKTSDSNEKWFASPKKGTYVLGPSG
                     LPLEPNEFNYQNNFIVINTLNYGITNAVSVGGGFELLTAALGHPIYYLNPRISIPLRE
                     NLHLGSGILFLSAPSLEINGTNVDVSKYYDLGVIYGSATLGNSDRNITLITGYGLLNS
                     KPTNRPTISLSGVYRLGSRIALISENWLIPDYYIISYGMRLMTRRIAFDFTLINNHEF
                     YANGTFGFPFVSFMIKLGKLRIEP"
     gene            complement(48094..48348)
                     /locus_tag="VGA21_12675"
     CDS             complement(48094..48348)
                     /locus_tag="VGA21_12675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651410.1"
                     /translation="MKTRSAITLENLQRMEQLRYESFLNAVEEAFRQTKTGIRHTDTA
                     MLKFADQLIGKFAAIYANTRNEELGRQVQEIILNKSQNYN"
     gene            48398..49309
                     /locus_tag="VGA21_12680"
     CDS             48398..49309
                     /locus_tag="VGA21_12680"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing transcriptional regulator"
                     /protein_id="HEX9651411.1"
                     /translation="MSIYVKLKMVAQAKILRVLKLISILKSRSKTIKQIASTLEITER
                     SVYRYLDLLTEVGFLVDKDFEDRYFIHVEEDEKSNFNFSTEESSLLKELISAGATNHP
                     LKETLLKKLYLNSDLKSISRNLEKARLGMLVGKINEGITLQKQVVLKSYHSANSNEIK
                     DRLVEPITLGELYDEVIAFDTSDRKVKHFKLERISSVIILSKSQRNTSSHAQAKSDIF
                     GIDGTSETWIKLRLNLRSYLLLREEFPKSIPYLKKEEEYLFNGPVRSFNGIGRFVLGL
                     MDSVEIVGPPKFKNYIKEKCKLMVESL"
     gene            49433..>49997
                     /locus_tag="VGA21_12685"
     CDS             49433..>49997
                     /locus_tag="VGA21_12685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M28"
                     /protein_id="HEX9651412.1"
                     /translation="MKTLKQVFAIIFLLPGFLMAQEKADLNAVYKIKQEGLDNSKVME
                     IARHLTDVSGPRLTNSPGLKRAQEWAVEEMKGWGLQNSGLEKWGDFGRGWEVQKNYVA
                     MTSPYYLAFIASPKAWTPGTNGLISGEVMMLDASTEGDLEKYKGQLKGKIVLFDTDAD
                     TSPSFEADASRLTAEDLAERAKQPIGGG"
BASE COUNT        14742 a         9814 c         9913 g        15528 t
ORIGIN      
        1 ttcacccgct ttatctttcc cccccagaag tatcttaaat ctttccctaa tattttctat
       61 tggttttgtt ttttccagcg tgtcaaactt taccctggac ttcggtttat actccatgga
      121 ttccaaatcc aatgtgagta tttgtgtatt accttttgaa tctttacttt tcttataaaa
      181 accctgacct gatttatctc ccagccaatt gttattctgt aatacgtcaa tttcggctgg
      241 cagcttaaag gtttctctgg attcatcatt cccgagcgcc tgatagagat tgttagtcac
      301 tttaatcaaa gtatccaatc ccacaatgtc agaagttctg aatgttgccg attttggcct
      361 tccgattaca ggacccgtaa gtttgtcaat ttcatcaatg gttaaattca actttttcat
      421 tgtctccacc accttcaaaa tagcataaac accaactctg ttggcaataa acgcgggcgt
      481 atctttacaa agaacggtag ttttacccaa gaatcgatct ccataataca taagaaaatc
      541 tgtgagggca gagtctgttt caggtgtagg aattatctcc agtaatggca gatatcttgg
      601 aggattaaag aaatgggtgc cgcagaaatt ttttctaaaa tctccgcttc ttccttcagc
      661 cattaaatga ataggaatgc ctgatgtgtt ggaagtgatt attgttccag gtttgcggtg
      721 cttttcaact ttttcaaaca gtgatttttt tatttccaga ttttctacca caacttccat
      781 tatccaatca cattctgata ttttctgcaa atcatcttcg aagtttccaa gacttaccct
      841 tgaaacgaaa tctttgtgat agaaggaatt gggtttcgat ttcctggttg attcaaagga
      901 ggaatttact atcctgtttc ttacagcggg atcttccaac gtcaatccct tttgaatttc
      961 ttcgtcattc agtttggaag gagtgatgtc cagtaatagt acttgtacgc caatattggc
     1021 aaaatgtgcc gctatccttg accccattac tcctgatcca agaattgcta tttttctgat
     1081 gactctgttc atcgctggtt tgctattaca tattcaaaaa tattattctg ctctatgagc
     1141 ttattgattt ctgttaatgt ttcaatcatg tttgtgattt tttcttcagg aattgaatcc
     1201 cggatgaccg cgttgaaaat ctttaccttc tctttcgcaa ttaattgttt ctcgttgcca
     1261 agtgtggtta gaaatatcct aactgacctg ccgtcgttct catgcttttt tctgcaaatc
     1321 agtccttttt tttccatcga ttttaatacc ctggttaagc ttcgagcttc cattcccatt
     1381 aagggtgcta ttttcattgc cggagttcca ttttcccgat caatgttcag cagaacaaat
     1441 gccatagtga tggtgaactc atacttaatg gcttcctgat tgtacatccg gtaaatggca
     1501 tgccaggatg cttttatatt aaaatcaata gtttcttctc ttttcatgct cttcttcttc
     1561 aaatgtaaga atatttagta tgcatgcata ctaaatattg gagaatttat tcgtcaagtc
     1621 gcaggtttca gtcgggtcat gcaacctctg atcatcctta tacgtctgat acataagact
     1681 tctatgtatt tgtgacttat gcattcatca gagctattaa agggaaatct gcaaaccatt
     1741 attctaaagg tgctttcaga ccaaaaaagg atgtatgggt atgagattgt tcagaaagtt
     1801 aaacaatctt cgatagagac cattgtatta acggagggtt cattatatcc catgctccac
     1861 aaaatggaag cggacgggtt attgaagatc gagttggagt atgtaggaaa acgtatccgg
     1921 aaatactact cgcttactga aaccggtgct tcacaggttg aaattaaact aaaggaattt
     1981 gcggcttttg taaaaacaat gagtgctgtg cttcagctga aactaacctg atgaaaattc
     2041 tgccggaaaa atatcttcac tacatcagaa aagatcttga atcgaaaggc ttaattcatg
     2101 agggacttga agatgaaatt gttgatcata tttctactct ggtggaaaac cgtatggggc
     2161 aagggaagcg ctttatggaa gcttatcagg atgccttaaa ggaattcgga ggggttggaa
     2221 gaatcaggca ggttcaaaaa gaaactattc aattcgaaaa ctataagacc aaaatcatgt
     2281 tacagaacta tttgaaaatt gcttaccgga atattgttaa gcacagattt tattcaatga
     2341 ttaatgtttt agggctggca attggtattg cttgctgcat actcattctt ctttatgttg
     2401 aagatgaaac aggctatgac aagcatcttg caaattcgca tcgcatctac agggtggcat
     2461 cggatcatct ttttcatggc aatgaatttc gtgcagcggt aactccggcc ccacttgctt
     2521 ccgcacttat caatgacttt cctgaagtag aggcaagtgc gcgattgaga aatggaggca
     2581 gcattgtttt tcgaaaagga gaatctgcca ttaaggaacc ttcattggct tatgctgatt
     2641 ccactttttt caatgtattt ccatttaatt ttcttgaagg taatccggag actgctttag
     2701 ttaagccaaa ttcgattgtt attaatcaaa ccacagcgca aaaatatttt ggaaattcca
     2761 acgctgccgg aaaaacattg cttactagtg aagggactga attgttagtt acagctgtta
     2821 tcgaagatat tccggttaac actcactttc aaattccaat attcatcact atggcgaatt
     2881 ggtccagggc aaataatgat aattggatga gcaacaactt ccacacttac ttattattaa
     2941 gagaaggata taaatcagaa gaactggagg ctaagttccc cgggatggtt gaaaaatatg
     3001 ttggtccgca aatcagacaa tttacagggg ttagcctgga agaggcggaa gccggcgggt
     3061 cacgcgcaaa gttctttttg caaccggtta ccagtattca cctggaatcc gacttagcgg
     3121 ttgaacttgg ggttaatgga gacatcactt atatgtatat ctttagctca atcgcaatat
     3181 tcattctaat cattgcctgc atcaatttta tgaatatgtc aactgcaagg tctgccggcc
     3241 gcgctaaaga agttggcatt agaaaagttc ttggttctta caggtctcat ctaatcagac
     3301 aatttcttac tgagtccgtt atcttaacct tgctggcggg gattcttgct tttgcttttg
     3361 cggtacttgc tcttccttat ttcaactggg tggcgggaaa aagtatttca atgccatttt
     3421 ccgatgtcac ttttgtactg gtaacccttt tcgggatcat aacggttgga gtactttccg
     3481 gattttaccc ttcatttatt ttatctgcat ttcgtcctgc ccagggaatg aagggagtat
     3541 ttaatgcagg gagaagatcg actaacatca ggagtatctt agtggttttt caatttgtca
     3601 tttctgttgt tttgatattg ggcacactgg tcatctataa tcagttaaat tatattcagc
     3661 ataagaatct ggggtttgaa aaagatcagg ttctggttat acgaaatgcc tatctgttgg
     3721 ataataacct tgaaccttac aagaattcac tggtatcaga gtctttggtt tcgggcgcga
     3781 ctatttcttc ttacttgccg gctggtggtc ctcgaagtga taatacgtat ttccctaaag
     3841 gaaatccgga actggaaagt ggagtgaaca tgcaggtctg gagtgttgat catgattatc
     3901 taaagacatt caagatggat attgcagccg gcagagattt ttcaatagat tttccatctg
     3961 atagatctgc actaattctt aatgaaagtg cagccgctgg ttttggtttc gaaaacccta
     4021 ttggagaaat tatctcagaa tattactggg atccaactac cggcgctatt aatcaggatt
     4081 ctatgattga ccatcaggtt attggagttg taagggattt tcatttttct tcactaagaa
     4141 ataatataag tcctctttgt ttaagactta accgaagtgc gggttccata agtgtaaggt
     4201 ttaacgcagc ctcggcgtct gaaattattg acttggccag gacaaagtgg ttttcattag
     4261 ctcctggcca gccttttgag tatttttttc tggatgatca attcaatagc atttattctt
     4321 ctgaaaaacg aactgggaaa atctttgcaa cttttgcagg actggcaatt tttgttgcct
     4381 gccttggctt gtttattttg tcagcctaca ctacggagcg acgaaccaag gaaattgggg
     4441 taagaaaggt tctgggtgct tcacaaaaaa gcatcatctt cctgctatca aaagaattcg
     4501 gaaaactcat tgttgcagca tttatcatag ctattccttt aggaatatat ggaatgaatg
     4561 catggctgtc tcaattcgct tacaaagcaa atccgggtat atttactttc ctgtcggctg
     4621 gcttggttgc tttactggta gctttcgtta ctatgagtta ccagtccttc cgggccgcca
     4681 gaactaaccc ggcggattct cttagaaatg aataaatcaa tggcttaatt ctgaataatt
     4741 ggggtattcg ctcttttttg cttttaagac gcaacctttt cccgcataaa gctatctaag
     4801 ttaaaaagct tatctatgtg gactaattac ttcaaggttg cttttcgcaa cattaagcgc
     4861 cagaaatttt attcattcac caacattttt ggactaagtg tggggatggc ctcgacaatt
     4921 ttgattcttc tttacgtagc cgatgaattg agttatgaca aattccataa ggactccgat
     4981 ttaatttacc gggttggcat ttttggccgg ctggccggtc aggattttaa cggagcaact
     5041 tcgccagccc ctatggcttc aaccttagtt gatgaaattc ctcaaataaa tgctgccgtt
     5101 agactgaata tctggacaaa agttattacc agatatgaag agttggcttt tacagaagaa
     5161 aaactgttgc ttgcagattc caactttttt tcattcttca gttttaaact tattcaggga
     5221 aataaagatg aagttttgaa aggtcccaat aagcttgtga tcactgaaag cgccgcaaag
     5281 aaatattttg gctattcagg acccggtgac caaactccca ttggtaaact gttgggtatc
     5341 ggaaatggtt cccgaacctg tgaagttgtt ggtattgcag aagatcctcc tgcaaattca
     5401 cattttcatt ttactctgat tatgtcgatg gattcctggg atgcttccag gcaaacacaa
     5461 tggactagca atagttttta tacctattat aagacgatac ccgctgccgg ggacatgaat
     5521 ccaacatttg aaagcctggt ggacaaatat gttggccctg aagtaaaaca atatctcggt
     5581 attgacatga atgaatttag aaaccagggt ggagcatata gttatatcct tgagccgctc
     5641 acagaaatac atttaaactc aaggcttgat aatcaattgg agccgggagg cagaaaagaa
     5701 tatttgtatg tcttcgcagc aattgccgca tttattattc taatcgcctg cataaatttt
     5761 atgaatttga cgacagcaag atcggcggac agggctaagg aagttgggat tagaaaaact
     5821 attggtgccc tgcgataccg cttaatgggt caatttttca gtgaatctat tttatttacc
     5881 ctaattagcg ctgtcatttc agttttgata ataatagtag ctctacccta tttcaacacg
     5941 ctgtccggta aggaaattgg tttggatgtt ctaatgaatt caaaatactt gttaggcata
     6001 ctcattctaa ttgccctggt tggaataggc gccggatcat atcctgccat ttatctcacc
     6061 tctttcattc cggctgaagt acttaaaggt cgtatccggg ccggattgaa gagtggtggg
     6121 ataagaagtt tcctggttgt gctgcagttt tcaatatcca taggtttaat tatatccact
     6181 cttctggttt atcagcaatt ggatcttatg caaaagaaaa acctgggatt taaaaaagat
     6241 aatatactgg ttgttgataa tgctcccaaa ctcggcaaca acaaaaaagt tttcaaggat
     6301 ttgctcaagc agaatcctgt cattgagaat gttagttttt caaatatgac acctccgcat
     6361 attgataata attccgtctt tcgcccgcta ggtgaaaatg ctgaggacaa tctatttttt
     6421 tatttcaggg ttgatgagga ttatgatgag ttaatggcat tggatcttgt gcagggcaga
     6481 ttctttgctg atgacatcgc ttccgattcc aatgcagtta tccttaatga agcggccatg
     6541 aaacaactgg gatgggttga ttatgaaggc aagcaactgc tttcatttaa cagaagtgaa
     6601 agtggagata tcatcaatgt gatcggagtt ataaaggatt ttaattttga atcgctgcgc
     6661 aatgaggtaa aacctttgct cttctttaat gcacctgatt ggggattggt gtctgtccgg
     6721 ctaaaagagg gtacggattt tcagtctgat attaatttca ttgaagaaaa atggaaggag
     6781 tttacgggta attctccctt tgactttgtg ttccttgatg aagattttga tgcattgttc
     6841 cgcgcagaac aacgaatggg caatattttc cttttgttta cggtattggc aattgccatt
     6901 gcctgcctcg ggctatttgg cctggcttct ttcacatcag aacaaagggc taaagaaatc
     6961 agcatccgaa aagttatggg cgcatccatg tctcaggtgg tggttttgct ttcaggtaac
     7021 tttacaaagc tggtattgat atcgtttgtg attgccacgc cggctgcctg gtacggaatg
     7081 aacaaatggc tgcaagggtt tgcttaccgg gttgagatta gtatacttac gattgccatt
     7141 ggaggacttg ttgcgctggt aatcgcctgg ctgaccattt caatgcaagc gctgaaagcg
     7201 gcaaagtcaa gcccggtgaa ttcgctgcgt gcggagtgat tattttttaa gccgcttctc
     7261 aaattcttct ttataagtgg ctcctaccgg cagaaatctt tctttaattc tcacctggtt
     7321 agcttcaata aattccatct ttgttaaatt tataatgaag gacttatgaa tgcgctgaaa
     7381 aattgccacc ggtagtttgt cttctatgtt cttcatggtt tcagtggcaa tcaaaatgtt
     7441 tttgccggtg tggagtttta agtaatcgcc gtaagcttca atataaaaga tatctgaaaa
     7501 tctcagcttg aataatttct tttccacttt aacaaaaatg ctatctgcat caattgcgtg
     7561 attcaactgc atcagttctt ttaccttatt taccgatttt atgaatcttt cgaatgaaac
     7621 cggcttcacc aggtaatcaa cagcatttac ctcaaaacct tccactgcat attccggata
     7681 cgccgttacg aaaattactt tcggagattt agtcattgtt ttcacaaagc taaggcctga
     7741 gagcttcggc atatttatgt caaggaatat caaatcaatt tccgtttcat gaataaattg
     7801 taatgcttca aatccgtttt tgcagacggc gctaagctta agcccgggag tttgaagaat
     7861 aaatttctcc agcacatccc ttcccagggg ttcgtcatct actatcatgc attttatgat
     7921 ttccatatct ttaaattagt gaaaaaaaga ttttctcttt cttcaatatt aaattcatgg
     7981 ttttcaggga aaacgagctc cagccgccgt ttaatatttt ccattccaat tcctccgggt
     8041 tgtttgtccc tgattgtttc tttttcttcc ctttcgtttt gaattttaaa gaatatttct
     8101 ctttcgttca cctgcaggtt tattattatt ttttgagatt ccttttctcc tttgattccg
     8161 tgtttaaatg aattttcaag taacggaaga aaaatcattg gaggtattct ccatgtaccg
     8221 gtttcacctg aaacattaaa ctccgcgttg ggataatcgg ttctcagttc ctggagtaag
     8281 atgtaatcgg taagcaaacg gatttcgtct tccagcagta tcgcttcctg cctgctttgg
     8341 taaattacat atctcaaaac atctgccagt ttaagaatat attctcccga ttgttggctc
     8401 ccattcaaaa ccatagagta taaattattc agggtattaa acaggaagtg gggattaatt
     8461 tgacccagta acgctttcat ttcgaagtta attttctcct tttcaagctc ggaaatacgc
     8521 tgacgttccc tgttaagctc aaaccattcc agcgagatct gcaataaagt caagatgaaa
     8581 agatgaccaa atacaaattg aattatttct atccaatcat aataagagat aaaataatat
     8641 ccgggcaaaa caaagtctac cccaacggtg aagaagaaat agttgaatcc tgcagcggcg
     8701 gaaactatta acatcaaaat aaacccgtat aaataccatt tccctgaatc cagcaagggt
     8761 ttcagccata gcaaattagc ataagcaaaa ggggctatag tgcattgaaa caggaaggta
     8821 taaataatat caatggattc tatctcattc ccccttacaa aaattcgcaa gagtatataa
     8881 aaggacagga tccaaaagaa agtgtgaaga agtatttttt ggatcttatt tctatctgat
     8941 gctaaactct tcattacatc ctggtaaata atcccggatc tatgtaggct tccttttccc
     9001 ggccaaattt cattacaaac ttctgaagtt catccgtact ggcagtaaga ataaagttat
     9061 tgcctgtctt ctcatgacta attctgattt tcttctgttc caataaatca attatgaaat
     9121 ccggatcaaa tggctcgatg atcatggcct tgttttcaaa acttattctc gcaaaagtat
     9181 gaactggaaa tagatgcatt tgaagccagt tgttctctat tcctttgata tctccatatt
     9241 cacttttggg gaaaatatca aggaataact ggttatccag ttccagtaat ctggctacaa
     9301 attcagccgc aatgctatct tccgttatgg ttaacagata accggataat gggtccggag
     9361 ttttacttcc caagaaccct tgtttaggcg gcagtttttc aaatttccaa ctctcacggc
     9421 catcccaaaa ttctccctca cctgcatttc gccagttgcc aaataatctt tggtcctcga
     9481 taagatctct ttcatgataa agtggatgta tagaaggaac gcaggaggta attattaata
     9541 atactatcag aaattttttc atagctatcg ctttttttaa caaataacga gtagccttca
     9601 gtccccgtca aaaattatcg atgaaccggt tcaaatcttc gatgaactga cctatgaacc
     9661 ggaacggtta atccaattgt tccattttcc aaaagataat acctccggcc tgcttaccta
     9721 gttccgcata agaaactttt ttaaatgtag tgagatcaat cacatccatg gtaccgggtt
     9781 cccctccaat tccctccact gaaataaagg cgtacttgct gtcgggactt atagctattc
     9841 catgtgaaac tttcctggta tttgaaattt tagctaactc tttccttgta gccacatccc
     9901 aaactcccgt agctccttct cccttatacg tcactaccac tttcaagcca tcgggagtca
     9961 catccacatt gtatggtgcc ttaccggact ttagtcggtg agttactttc cacagctttg
    10021 tatctatttc aagtagttca tccgatccat ttcctgcaac ataaactact tgtaatgtcg
    10081 gatgcggcat tgcccaggtg ggtttggttt tggaatgaac catttttttg gctgctccag
    10141 tttcataatc cattgctgaa tgatccatac ccccgctcat tgtcatttcc ttctcatcaa
    10201 gtaccagcat tcgggagact tccaggttaa ctgtatttat ttcaaacaat tctcctgaca
    10261 tcatcgcgac actgtactga agcagcccat tagaagaaat gcgtgagccg tgtggcatta
    10321 cgccggtttc tatttgtttt aattcggtca tggtttccgg gtcaactacg gaaacagtac
    10381 ttggaaccat atctccatgc agattaaagt ttacacaata aagtaatccc gttgctttgg
    10441 aaacttccat ggatgccgga aataaaccaa ggtcggtttt gcccactaat tcattcgttt
    10501 ctgtggagta cttatacagt gtaccgaaag ggttgccatg tgccacagaa acaaaccagt
    10561 attttttatc aaggctcatg gttagtccat gaggaccctc aatatccgcg ggccagattc
    10621 caataggaat agtcttaatt acttccgctt tcttgccatc aaatttgatc agtgaaacct
    10681 catcttctga ttccgccgtt acatacacgt agtagttttg agcagagagt tgagcgagag
    10741 aactgaaaaa aattatgagg tgatacagga ttaatctttt cattctaatc gttacttgga
    10801 ttggatttga gtttccttag gtttctctgg ttccagcctg acagcccaca atccggaatt
    10861 ccaatctgaa atatatatgt gtcctttata tggctgggct ccccatgtaa atggagcatt
    10921 cggaacaaat ccatcgggat ctccagggat tagccaaccg acttcacgtc cctgattaaa
    10981 cagatcgccc atcaattctc ccgaaatatc cactactctg actcctccat tataaaaagc
    11041 gacgtacaac atatcatttt caatccacaa attatgggac ccggcgccgg gtacttcaaa
    11101 tcttgcaact tcaaccggat tctccagatc agtaaaatca ataatatgta taaaaccgcc
    11161 ggcaacattg gctcctttca ggttcagacc atatgggaat atttcgtccc cggcaaatac
    11221 ataaaatttt cctgttgatt tagacctgta gggaaatgtt gcatgattgg ctccgcttgg
    11281 gtaagcataa ctggcaaatt gcaccggatt gtccggcgtg ccgcctgcga ttccatttcc
    11341 aacatcaacc aattgaacac catcgcccca attggaagaa tatgcaattc catcttctat
    11401 ccaaacatca tgaatggaat ggccgggggt atccagttca aactttccgg caatttttgg
    11461 attcttggga tcttcaatgt tgatagagta gtatttttga cctgcactta gtgcataaac
    11521 atgattcttt gaaatgaaaa ggttgtggac tccacctgtc agattttcag taaaggttga
    11581 aataatagaa acatcccttg gatttgagac gtccaggatt acgattccat ttttcctgtt
    11641 ggatgcccct tcccttgaaa taatgcatat tttaccatct tcagaaactt taacatcatt
    11701 aactgttctt gcatctactt gtatgctgtc aatcttaacg atatcggaag gatccgttac
    11761 atcccaaaaa taggcggtgc catcagctcc ccaggttccg gtcacaccat aatctctccc
    11821 atccaaccct tcccaaatcc aaaaatcaga ggtgtgttta ttggcgacag accctttgcc
    11881 tactcgttcc acttttcttt gaactccgcg atcaattatt cgaattgttt tttggtctgc
    11941 aaaatttccg ctcttagcaa taagagtata cattcccggt tcctctgcca caaaacggcc
    12001 gtcttgttgt attaagccgg aagcggtcga tgaaacatca aaagctctgc cttcaaatga
    12061 gaaataaact ggaatatcgg ttagcttttt cccactctta tctgtggttg ttgcttccaa
    12121 tttaatcaca tctcctgccc gggcctcgga aacggtagtc ttcagtgcta cttttgctgt
    12181 tggattttca acaaccataa ccgtgacctc tgtcgcaatg gcttctttct taactgatat
    12241 agtagtttta ccttttttat ttgccaaaac atttccaaac atatctaccg aagctatttc
    12301 aggattgcgt gaactgtatt ccaaatcaat gtttattctc tcaagatttg ccgcatctgt
    12361 aacctttgtg cggataacct gattggtgtt ttcataaatg ctggaaggaa aatcgagaat
    12421 ttcgatcctg gaaattggag gataattaac aatgaccggg atatccaccc ttgctacatt
    12481 accatcactg gctggagaaa ttatgatcaa gctgtattcg ccaggtagaa tcccggtaac
    12541 aagaccggtt gaatccaccg aaaccgctcg tcgttttctg gaaaagtacc gcagagtccg
    12601 ttcctctatt gttctgttgt tttcatcaac tacccgggca ttcaaaggca agctttggcc
    12661 aacatctatg gtcagcggaa ttttatcaac ttttattgaa actttactct gggcgttggc
    12721 tgaacaaaaa atccagcaac acagtaaacc tgttgcaatt cttttcattt taatacgtga
    12781 atttggttga gagattgaat ttaatgaaaa agaatgagct tacgcaaact taaatttatt
    12841 gcggcttcta attgatgatt ttttcggacg ccgaaaagtc accccgtagc ttacggaacc
    12901 tctttctggc atcagtggca aaaatgcttc cgggataacg caccaggaaa tcctggtaaa
    12961 tctccatcgc cttttcctta ttttttaatt gctcctcata aactttcccc aaggtgtagt
    13021 aggcattatc acccaggaca tcataaccat attcctcaac aatcttctcc aaaagagtta
    13081 ttgtttgatc gaattggcca gctttcataa gaattttggc cttttgcata tataattcat
    13141 ctgccagagt gtggtttgaa aaatggacga gcatcgaatc tatctgttga attgcacttt
    13201 cagccttgtt ttgaaacaga agtaattcaa tcgcggcgaa ttttttcatg gccgcattcg
    13261 ttgtatcagc atccaatccc gtattttcaa caatgagtaa gctcaaatcc atggcatcat
    13321 tggctatttc ccttgttgtt gctgttttca aaatgttcaa atggtctctg gccaaatcaa
    13381 actccccttt ataataagac agtcttgcat tcctcaattt ggcttcatat ccagattttg
    13441 attctttcat cgacttttca acctggctgt atagcaatgt ggattcccag gtttcgcccg
    13501 taagtatgta gatatctcca agggtcagct tagattccgc taccaattcc tgattcgcgg
    13561 cagacgactt gattatttcg ttcaatattc tgatggcttc ttccttttca tccaaataaa
    13621 aagcgtagag ctgcgcctga tttctcatgg cctccaaagt tgtggtatta atcccgatat
    13681 catttacaaa ggcatcataa tcatttacta aagcctttat gtcatcaatg ttgacgggat
    13741 aaaacttttt aacgatttct tccctggatt taattcgata catcctgcta acgataaaat
    13801 tgaagctgcc tggatattct tttatgatat aatcaaagat tttgatggct tcttcataat
    13861 cctcattgcg catggcaatc aggccaacat ccagaactct tcttccatct gacttggctc
    13921 gcttatccaa agctcttgcc tgaatgaatg caccgtaaaa attcttttgt tgcaggttta
    13981 cccagatcag caattctgca tataattcat ttccgggttc actttgtacc cgctcataga
    14041 gcagcgtttc gagactttct aattcatccg gttcagataa taaattttga agggtgtttt
    14101 ttacatagtt taagttggag gggttttgaa caacataatt tagatattct cctaccattt
    14161 tgtctttttg attctgcatt ctataaatat tcgccatctc caacgagaag agcaaatcat
    14221 tgttcatagc tcgcctgcct tcattatagg ttatcactgc ccaatccatt agttgtttgt
    14281 tgataaaata ttgtgccgta attcgaacct tataattatc atctttaatt tttgagataa
    14341 gcttcctgaa gtaagtattc gctttttcac tatctccctg acgaacataa agagtaccta
    14401 tatccaactg ataataaaga ttatccggat acctttttat aactttatcc aaataagact
    14461 ccgcgccact aaaatcacca agctcaacta aaacattaaa ataattctga tggatgtagg
    14521 gaatattctg ggtatttctt gcaagctgct catacatttc tctggccttt tcaagctctg
    14581 cttttgcgta gtattcattt gccaattcta tctcattggt attttgagaa aatgtgttga
    14641 atgctaagaa caataaaagg aaagcaagtt gaaatttcat atctgatttt tccacaatat
    14701 tcatacccat aataataata taactataaa tgtatattta ctattgttat tattaggttg
    14761 tgatttatgt tgaaaagtaa ggaaagttaa tcttgataaa ttgatttgtt tttaagtctt
    14821 cgcttattaa catagatagt tctgttaaag agctgattat aagtgaatta acatatcctt
    14881 tatgttaggt taaagttgat tgttgaaaat gataactcag ttttgactgt gcaaaatgtg
    14941 gatattcaat tttttattaa cggtctgctg aaataattct gaacatttac tgaaattttg
    15001 gaagtttgga ttttatcaac aaattgtgag gttgaattca actaattatc cacactaaga
    15061 ggaataagaa aaggtccgag gacccagttt tttttaagag taattatatc cgttttggac
    15121 tcttgatcat aaaagaaaat aacgggttct ggcattattc gatttctaaa attgcctggt
    15181 ctccaaactc catccgcatt tgcatcgatg agtacgcgaa tcttatattg atcagcgcca
    15241 aggtactcca ttagcactct ggaatttccg gggtagactt tttgcaacac cccttttgca
    15301 ctcaacagat gaacgttgaa tggaaaagaa tcaccaggaa attgaagttc aattgtacca
    15361 aaattatcag gatttaaaat agcaatgggt tttgtcatta aagtattcgt atctgattca
    15421 atactttgta gagaattaat attcattttg aatgaaaggt ttttcattaa ggtgagacta
    15481 tcaggaagtg tagtgataat ttgtaattgt aaaggattag gctggaaaac ccggctgggc
    15541 ccaaaatctg gcaatggaat ggacactgaa tccaaattaa tagtccataa agtgtcgatg
    15601 aattttttta taggtttagt gaattcaatt gtggcgataa attctgaatt tattgttttt
    15661 aaattggcca tagtgatact cgcgctgaaa ggttcctttg gtcttgaatt cggcaggaaa
    15721 gaaaaccgga tggtgtcggt aattttttgc tcagcactat caagaactgt taaggaaaat
    15781 tgtatctctg gattagacgg agaggtatta tagattctaa ttgctttgtt ttcatcaata
    15841 taatgatgat aaagagagtc ttgttggtta tagagctgaa taaaataaga ggtagttctt
    15901 tttgaaaacc ttacctcata atattgtcct gcctgtctgg ctgaagttat agttaatggt
    15961 ttaatattaa aaggaaaagg cttcaggaaa atgtcagagc gcgttgaatc caactccaga
    16021 atttcattga gaaaagcgaa aggttcggat tctgattggc attttagatc gttatttttg
    16081 tccttccagg tatagattgt gtaataatcg gacttgatat tcagaaattg aaaatctcca
    16141 tcagtattag tttgggtggc atatagtgga gcggaattaa aaatattgag tgtgtcattc
    16201 gccgagtaaa tagcaactgt gtagttggct gccggttcgt ttgttaataa tgtaatgaca
    16261 tttccggaga taccgagaga gtccaggtag gaaccggtgc taaaaacaag cactaaattt
    16321 tcaacagagt tactttcagt tacatcttta attgattcac gaaaattcaa ggtataagtt
    16381 gtgctgtcat taaaagcttt ttcaaaagtg atttgcagtt tattccggtt tattttggaa
    16441 ttaaatttcc cattaacaga aggggtgata attaattggc tttggagatt tgcctcctta
    16501 atcatttcat tgaactggag ttcgattacc tggcctttga aatttaaagc tccattttca
    16561 ggtgaggatg atagcagaat gggtggattc tcgtcctttg gaccaccggt tggttgagtt
    16621 tggttggcac agccccaggc aattacggca atgactatag ttagatgaac tttaattttc
    16681 attttctgac aatataaacc aggctcgaat aattatcaga gtttttagcc gcccatgaat
    16741 tggaacggaa tcctgctatc atgcctttga gaaagggtaa ggaatgtttc aaatttttct
    16801 cacttagaat tgaaacataa taagcatcca ttttcagagg gattatattt tggagagtga
    16861 gaccattgcg tttaagtagt ttcttcatcg ttttgggttt gaaatgatta aggtggcgag
    16921 gtacgtcgta accggcccaa tcctgaccat attttaccgc gtcgtaggag aggatattgg
    16981 gagtggcgat aatcaattta ccatcctttg aaagtaacga ggccaattgc tgtaataggg
    17041 aattaatgtc ataaatatgt tcaaggacat gccatagagt gatcgcagta aacacctgat
    17101 tatgagtaag ttgtgaaaga ttttctttaa tatcaagatt atatttctga ttaccgaatt
    17161 cgcgaggcct gccatgaagt tcagtacctt caacttccca ggcattactt ttcattttta
    17221 gcaaaaactc tcccgttcct gtaccaaaat ccaataacct tccgtgtgga gaatactggt
    17281 taatcagatt cttcttccag gtcaggttaa tagatcttac ggtcttataa atcaggttga
    17341 aaatgttgga ggttttacca gagtgtggat cgtaatgatc cgattcataa tagcggccta
    17401 tagactcctg gtttggtctg gggtttgtta agcggagtcc gcaagactcg caatcagtta
    17461 gcacgaaact ttctccagac accaggaagt cttgacagat tatttgattt ttgaactttg
    17521 agccattaca gacagggcat tccagaatct tttcaaacat tttacttgcc gaggtaaacc
    17581 attataattg aaatgtccgc aggtgagact ccgctaatac gggaagcctg accaagagat
    17641 tttggattaa tgaacttaag tttttccctg ccttcagcgg atagtccttt tatcctatca
    17701 taattgaatc catcattaat tatgtaatgt tccaggctac ccattttatc caccatttcc
    17761 tgttctttct ttagatatga atcgtattta attagaattt cagcctgttc tttaccttca
    17821 gaactgaatt tgcttaacct cgactccagt ttatggtcgt tcttaagtag cccctgcagt
    17881 gagatctcag gccttcttag tagattctca acacttacct tttctttaat tgtggccgta
    17941 attaatgaag tcagggttcc gttaatttcc gttggggtaa ctttcagttt tgagagtgta
    18001 gctagaagtt ctgtggtttc attcttcttg tccaataccc tttccagtct caaatctgag
    18061 gccaaaccca gcctgtgacc ggattctgta agcctgagat cggcattgtc ctgcctgaga
    18121 agcagcctga attcagctcg tgaagtaaac attctgtagg gctcatccgt ccctttgtta
    18181 atcaggtcat caataagaac tccgatatag gcatcagatc ttctaagaag gaaaggttct
    18241 ttattatgta ctttgttatt agcatttata ccggcaatca atccttgtga agcggcttcc
    18301 tcataacctg ttgtaccatt aatttggcca gcgaagtaga gattttgaat ccctttagtt
    18361 tctagactta agtttagctg ggtgggcggg aaataatcat attcgatggc ataacctggt
    18421 cggaacattt ttgccttttc aaaccctgga atttgctgca aagcggcata ttggacatcc
    18481 tccggaaggg aggttgaaaa tccattcaca tagacttcga cagtattcca tccctcaggt
    18541 tcaacaaaaa tttgatgtct gtcccgttct gcaaaccggt ttattttatc ctcaattgat
    18601 gggcaatatc gtggtcctat tccttctatt ctgccggaaa acatgggaga tttatcaaat
    18661 ccggattgaa gaatttcatg tactacggga ttggtatagg taatatgaca acttttttga
    18721 acagataaag gctgtgtatc catataggaa aacttaccag gagtttcgtc tcccttttgt
    18781 tcttccattt tggaataatt gagggatcta ccatcaatcc ttggaggtgt tccggtcttc
    18841 atccttcctg actgaaaccc aaatgataca agctgttcgg ttaatccggt ggccgctttt
    18901 tctcctgttc ggccaccgcc aaactgtttt tcacctatat ggataatacc atttaggaaa
    18961 gttccgtttg ttagaattac ggatttagct ctgatctcaa ttcccatcgc tgttcttatg
    19021 cctatcgcat ttcccttctc tattacaatt tccttcacca tttcctgcca aaaatccaga
    19081 ttttcaattt tttccagagc gaggcgccat tcttccgaaa atcgcaggcg gtcattttgc
    19141 gttcttggac tccacatcgc aggacctttg gacttgttca gcatccggaa ttgaatcata
    19201 gacttatcag agatgatccc tgaaagtccg cctaaagcat cgatttcacg cacaatttga
    19261 ccttttgcta ttccccccat tgcaggatta caagacattt gggcaattgt ggtcatattc
    19321 atagtggcca ggaggacctt gctgcccatt gttgcagcgg cagcagcggc ttcacaccct
    19381 gcatgaccgg cacctactac aattacatca tattctctaa acatactatc ccccgttcca
    19441 cgtgaaacat taaaacttta gttttgaaca cggtttgtgt tccacgtgaa accttttttt
    19501 taatacaaag ataaacaagc ttcctctctt ttgcgcattg ccgatttttc gccggtagtc
    19561 ttatcagaat accccaacag gtgtaataca ccatgaatca ttactcttcg cagctcttgc
    19621 tcaaaggttt tcttgaattt tgttgaattt tccttaatcc tatcaatgct aatgtagata
    19681 tcagcgctca atactccctt cttcgagtag tcaaaagtca ggatatcagt ataggcatta
    19741 tgcattagaa atttttggtt aagactaagc aaaaaatcgt cagagcaaaa gaggtaatga
    19801 atcccggcca actccctttt ttcattccta atgataccaa gaatccattt acgcgtcttt
    19861 tgcggattag gcacctcaaa ttctatatcc tgagtatgaa atgagacaga accattcatt
    19921 tctaattaat ataaaacacc agttctacag tgcgaccatc atcaataaaa ttaaccccat
    19981 cgcagaggtg tctcataagg aaaatccccc taccgcctgg tttggagagg ttttcaggtg
    20041 aggtagggtc gggcaaattt cgaaaatcaa atccggaacc ttgatccata attcgaaata
    20101 caatctgact ttcatcaagt tgaagcgtta tgctaacatt ctttttctta tcattcttat
    20161 tcccgtgctg aatggcattg tttacgccct cggttacagc taccattatg tttccataga
    20221 tatcatcgtt aagattaaac cggtctttgg cattatcaat gaaactttca attatgcgga
    20281 tattctcaga caatgaaggg atttcaattt tgattgaatt catataatgc cagggaatat
    20341 attagttctc tccaattcgt ttgaagtatt catttacttc tttcttataa taagggaaga
    20401 gtttgagggg taccgttttt aataactcaa cttccttttc tttgagctta aaataatctt
    20461 caaaagcctt tggtacaagg ttttcgtaag attttgctgt ttcagcttca cgttcatcat
    20521 caaggtcgcg ttcacgtatt gagttttcgg catccagcag ccgtgtcaga atttcatttt
    20581 gcctttcaat agtttcttca gtcaaatgtt tgttaacaag atctagttct gtttcctcca
    20641 tctgattcat caattcctga agctcacctt gcccctgccc ctggtttagc ttttcttctg
    20701 cctcctgcaa tgcacgtcga atcctttcct gctccgcggc caattttgca agctcctcgc
    20761 tgagttccct tccagatttg ccactttctt ttaattgctg aatttgctgg ttcaattgct
    20821 gctgcaattc cgtcataccc ggcattctct ggtttccttt gcctttctgg ggattaccca
    20881 tggcttccga catagcttgt tgcatttgtt gcagaacgtc atccaacatc aaggcaagat
    20941 tgttcatgga agtcatggca aattgctgtt tacctgtggt aaccggaatt acgttccttc
    21001 cccggtctct cagggcttcc attgcctcat ctatatatct gttcatttcg cttacctccc
    21061 gggtaacgaa actttgaatc tgaaaaaccc tgctggcaag ggaaagtaaa ctgtctttta
    21121 caattttaga ctcatcttta atctttagtt gttgctgaga gaattccaca aaccggggat
    21181 cgctttgatt gatttcccgg aaatcattca tgagcttttc ctggtcaaaa gatagctgaa
    21241 gcaaattgtg aagaatgtct ctcaaattct ccatgttttc cgtcatggtt tccatttcca
    21301 taccgccctg catttcctga agcttttggg agagttgttt catttgttgt gcggcttttt
    21361 tctgtgagtc ctgagctttt ttgttttgat tctgcttcag gttctcaagg ctcttttgtt
    21421 ggttctcctg aatttgctgt tgttcatcgt tgaagtcttg catagactgc ggattcttca
    21481 aattctggtt caaatcttgt atttcttcaa tattttcctt tatttgttca aaatcagtgt
    21541 tcaattcttc ttgttgcccg gttaggtttt cattttcccc ttcgttttct cctgaatttt
    21601 gactatcctt ttcttcggcc tcctgattta ctgaattctc ttcctgttgt gaaccttcct
    21661 cttgctttcc ctcctcgctt ttttgttcca tctcttcttc ccctactttg tcctctccag
    21721 ttttccgggc aagttcttct tgttcttccg ctaattcctc aagttcatgg atagtttcgt
    21781 tcagcttata ttcaaatttc atttttttga atagctcaag agttcgctcc agttcttttt
    21841 caaggttatt ttccttctgg tttatcctat ttataagctt ttgcatttct tcggatgcct
    21901 ctttttcctc aagaagtttt tgaagttctt cataaagttt tcgtgtttct tcatccagca
    21961 aatcatccat aagcttttga agttgctcca ctttttcccg tattcgggca ttttcttcat
    22021 caaatcgctg ccgcttcaaa tcattggctt tattttcctc actcagtttt ttcaatgctt
    22081 cttcgagttc atgtttcttt tgcagaattt cattgagaag attttcatcc tgccaatcca
    22141 tatccttttt cccgcgaagt ctgttttcag cttcctcaag cttcttccgg aggtcttctg
    22201 tctgctttaa tgtttcttca attttgtctt ccgatttgct ggtggcagct tcaatctcct
    22261 ttttgatctc atccctggtt gggatcatga aggtgtaggt tccagttttg gtggattttg
    22321 ctccgtttac cccatcatta tcccacacct gcaaataata attgatcttc tctccttcct
    22381 ttatattcat atcaccaagc gtccattcat aataaaaact ctggttattt tgatggctaa
    22441 ccagcggtat tggaatgctt tgccaatcag atttgttatt gtttctttca atttcataaa
    22501 acagacccaa ccggcttaat ccatagtcat cttccagata accgccaagg atgaggaatg
    22561 aatacagaac cgtatcctga aagatgttca gatttatttc cggatattgg tccggaatta
    22621 cagacactga gtagtttatt ttttgaagat tttctgagaa tttatttcta agtttaattt
    22681 catattcttc agatttaaaa aactgctttt tcgcctcaac aaactgatta tcaagatttt
    22741 gaaatagaat gtcgttttta gaatccttga aatcgacttg taattccatt gcttccagtg
    22801 tttggaattg ccatgttacg atggaaccct caggtatttg caggttacca acatttgaaa
    22861 acctttcggg gtccttccca agatatgacg ggtaattaat gtggacgtca aagcttttca
    22921 ggtttggtct gctagcaatt ttaagttcaa attcaaagct tgtgaagccg gcactttcga
    22981 aataaaatga cttttcagcc tgaatttttt gaaaggtaaa ttcgaaagac gaagcattga
    23041 ggcggttcat tttcatcttc cttccattat tgaccagata aacattttcc gggaaggctt
    23101 ctcctgttaa agttaattca attgtgaaat cttcattttt aaacgcgagt aatttttggg
    23161 aatcaataac aaactgaaat ggagcggctg gaataaactc tctgctgaat tgaacaattc
    23221 tctgggtgct ggttgaaaac aaattgggca gaacaacggc cagaattagg gcaattattg
    23281 cgggaggaac gaggtatcga agatactttc ggttgtgacc aaggtttatt gccgttgcaa
    23341 aggtgaaatt tctgagattg ccggattttt gattaatgct ggctgaaacc aattgattct
    23401 tcccattatt cattttttga agctgaataa tgttcagcag tttatcccga atttcaggaa
    23461 aaaaaccacc tatcattctt gccgcgtctt catcgcgaag tccattttca gggtcattgg
    23521 atttaaacaa aggaatcagg ataaagtaaa caagcaaacc gattgaactg ataacgaagg
    23581 caaagaaaat taatcctctt attattgagg aaaacctacc gacatactca accaaatttg
    23641 ctgcaagaaa gaaagtgaga attattgaag cagttataat gcttccttta agaataagat
    23701 ttagataaaa ctttttcttg tattctttta ttttttttgc taacccattt actgattcac
    23761 tcataccgca cctgaatttc tattcattct actctaaata tagcaagaat aggcttaact
    23821 gactttaaag gggtcaaaca ttgtgcctca ttactaaaac gaatgatttt gaatgatgtt
    23881 tcccaatttc attccatcat tctttcattt ggccaaactt ctgattcgaa atattcaatg
    23941 acatgcattt tcaaaagctt ttcctgctcc aaaagatcga aggccggcaa cttcttaaca
    24001 agtttccaat gaggccatcc ttcttcatca tgtccctcta attggtaaaa accagataag
    24061 ctgagcactt tacagatggc gatatgcatt atatcctgtt tctgttcttt agagaaagac
    24121 ttaggcccct tacccaattc ctgcaagcca ataaggaata ggacgctatt cagatcggcc
    24181 ggttttttgc caattctatc ctcaagtaat ttgagtaacc gttgccatct cttttcattt
    24241 tcgtggtcct ttttaagcat ttgatttttt caattcttcc caatactcca cggcccttct
    24301 caaatgagga attacaattg ttccaccaat cagattggca atcgcgaaaa cttcaaacac
    24361 tgctttatca gaaactgata attcaaaaca tttacccaaa tggtacctga cgcaatcgtc
    24421 acagcgaagc gtcatagaac aggcaaggcc tatcatttcc tttgttttca catcaaggtc
    24481 accttcctgg aaagcatttg tatcaaggtt aaaaattctt ttcagtatct tgttatcaga
    24541 atcaagaatg gcatcattca tccggtggcg gtagtcatta aagtcagaaa caatactcat
    24601 tgtgttattt attattaaac ttgaaattat aaaatataat tggggtcatg attagagatt
    24661 ttgtaaatct tttatttcca cagtggtgcg cgggttgcat gggtcattta gtttcgggtg
    24721 aggaaagtgt ctgtacattt tgtcgttcgg aacttaagat atcttcaacc tataaggaaa
    24781 aaccaaatgg aattgtgagc aaattttacg gcaaaatacc tgtggaacat gcattcacca
    24841 tgttccaatt cagcagaaaa ggagtaatcc aaaatttgct ccatcgaata aaatatggcg
    24901 ggtattatga tatgggagtt tggttaggga atttgcttgg agcggagata aaacacgagc
    24961 cctggatttc tgacttaaat attatcgtgc ccatacccct tcataaaagt aaactccagt
    25021 taaggggcta taatcagagt gaggcattcg cccgtggatt gtcaggagca atacaagttc
    25081 ctattgatga ggtcatggtc aggaaacaac acactcaaac ccaaacacgc aaatcaagaa
    25141 tggagcggtg ggtcaatgtt tctgaaattt ttgagttaac cagagggaaa tttgttgagg
    25201 ggaaaaatgt tttattggta gatgatgtga ttacctccgg ggcaacattg gaggcagctg
    25261 ggtcagttct gataaaaaaa ggagtcaaca aaatttatat tgcaaccatc gccaatgcat
    25321 aaaaaaagcc ccagaaaggg gctataaatt caagttgaat ttcttttctt tagtaattag
    25381 atccggccct aatcattgcg catctgaaac caatggttga agtagctgaa tcttcttcca
    25441 gatatcttct ggttccggga gacagccagt aagcaacgtc tttccaggat ccccctttat
    25501 ataccctgga tttattacta ataagtgatt gccagtcatt gtcatcatac ccggtatcat
    25561 attgattttc ctcatccaac cagccatttc tacgtgccgg gtttaaatcg tccacatctt
    25621 caaatgaaag tggtctgtaa acatcccaaa cccactcatt cacgtttccg gccatattgt
    25681 ataacccgta atcattggga ggatattcat aaatgtacga tgtaatcatt gcgccatcgt
    25741 taagcttacc ggcgatacct gcataatcac ctcttcctct tttaaagttt cccaggaagt
    25801 aacccatatc tcttccataa gggttcctta atgcatggcc atcccatgga taaagtcttt
    25861 tatgagtttg attttcatcg agccattgtg ttccaatgag tgccatcgct gcatattccc
    25921 attcggcttc agtggggaga cggtaattgg gtaaaacaac accggtttcc agtggaattc
    25981 tgccttgtgg atatgcggtg tagtcgattc cggcctgttc cgcaagactt tgatttactg
    26041 cagcggttct ccattccgtg tagtcaattg cctgaatcca actgactccc actaccggga
    26101 aatatctgaa gcccggatac ctgaagtaat gatcaacata aggatcgtta tatgccaatt
    26161 ctctggacca gaccgttgta tcgggtaatg cagctaaata atattccctg gtagagtctt
    26221 gttgaatata atacagatac tcaagccagt ggatattagc tatttcagtt tcatccatat
    26281 agaaagaagc gacagtaaca gttctttcta tattatcccg tgaattcatg atatcctgtt
    26341 cggaagatcc aagaacggtt ctaccccctt caatgaaaac aaggttgggt ccatccggct
    26401 ggccctcgaa aggactaacc tgaaatccac cctgttcatc attataagcc aatccggttg
    26461 ttgtactgag tttccccggt ttagtgctag taggtcgtga tccaccacca aaaatatttt
    26521 ggatgaactg gcaggagccc aacaacacaa cagacattag gataatgcct atgtttctga
    26581 acgctctttt taaagaattc atagccaatt tgttaagatt catagctttt ataacgtact
    26641 aatatagtaa tataaatagt tagcctcaaa ataattagag cgccaaaata gtattttaat
    26701 tagattcgat taccaattac ttataaaatt tcgctgtttt ggactttaag taattgcaat
    26761 attgtaatgg taataactga tagtctgggt aaatattata caatgcacca ccattttttg
    26821 tcaggcaaaa cccaaaatga ccttttatct gctcatatat ccaagaattc aattccggat
    26881 attaatacat cacaatacac actaattttg aataaatgga agagagtata agacagcgga
    26941 agaaagcaag actttactgg atattaaata tttccgggtg gtgtatattc cttgtagttt
    27001 attcatggat cgccgtaaca atgtatctgt ttcattggaa gattctcctg gcatacttta
    27061 acagtacaat tattggcttt tctctgaccc atttcctcag actgttcatc aagaagaaag
    27121 aatggatcaa gctgccaatc aggcgcctgg ctttatatac tgttgtcgcg agcctgataa
    27181 tgggagcaat ctgggccttg ataacatatc caattaatta ctccttgctg ggacttaatg
    27241 agctggaggg acttgataag tttagaaatg agaacatagg cttggtaaat acaattctca
    27301 ccgttcattt tcaattttcc cttttaatgt tcatctggtg ccttctttac tttggaatca
    27361 atttctttta ccatttcagg gagagtgaag ttgaaaagtg gaaattaaac gcgatggtgc
    27421 gggaagcaga acttcaggct ttaaaatctc agattaaccc acacttcatt tttaatagct
    27481 tgaataatat ccgttccctg gttctggaag atcctgaaaa agcccggtca atgataacaa
    27541 acttatcagg tttattaagg tattcaattc agttcagccg caaggaaaaa gttaaactgg
    27601 aggatgagat tgaagttgta aaaaattacc taaagctgga atcaattcag tatgaggaca
    27661 gattgcgata caaattggag attactgaag aagcgcttga tttcaagatt cctcccatga
    27721 ttattcaaat attagtggag aatgcgatca aacatggcat cgctgcatta ccggaaggcg
    27781 gagaggttaa tatacggact cacttatttg aagatgacct ggtggttgaa gtgattaatt
    27841 ccggaaggta taaaacagaa attgcaggta caggaattgg tttacaaaat gcctctgaaa
    27901 gacttcgcat gctgtttgga aaactctcca ctttaaccgt agaaaatctt gacgataacc
    27961 gcgtgatagc aaaattttca gttccaataa ccatttgaaa tgaaagcgat tataattgat
    28021 gattcaaggc tggcaagaaa cgagctcaaa agactactaa ctgattttga ttttctccag
    28081 ataatagggg aagcttccaa cgctattgaa gcgaaagaaa aaattgaatc gcaaaaacca
    28141 gatgtaattt tcctggatat ccagatgcca ggaaaggatg ggtttgaact gcttgaggaa
    28201 ttggaagtaa ttccgcaagt tatatttaca acagcctacg atcagtatgc aataaaggct
    28261 ttcgaggtaa acgcattgga ttaccttcaa aaacctatag aaaaggaccg gctgtcagcc
    28321 gctattaata gagtagttga aaggcaaaat gaccaggcgg caaaaggtga ttctgactat
    28381 cttaagccta cagatcaggt atttgtgaaa gacggagaaa gatgttggtt tgtattgttg
    28441 agtgaagtac cgttcttcga ggtagatggt aattatacta aaatcttttt caaggatcag
    28501 cgacctatga taccgcgtac attgaattac ctggaaagcc gtttagaccc caaattcttt
    28561 ttcagggcca acaggcgaca gataattaat ctttcatgga ttgaaaaaat agaaccctgg
    28621 ttcagcggca gcatcagaat ttacctgaaa ggaagtaagg aaatagacgt ttccaggcgc
    28681 cagacccagc gatttaagga actcacagga atttagcccg tttcccgcat gccaattgat
    28741 tttaattgag gtaataaatc cgactaaaga gccagaaatt accccgaaaa tggggattac
    28801 tttgccgcat ttttccggat aaataggtag caagttaaaa ggtttgttat gttggacaga
    28861 gcaaagtttt gccgaatgag tttcaaggaa aaatgttcgg aaataacctt tcacgggaaa
    28921 ttattggcaa ctaaaaaact tggaaatcac caggtattcc tattttggtt aaagagcttc
    28981 tacgttgaac tttggttcaa taatagaaaa ggcgaaatac atggaataaa ttcatttgaa
    29041 tctgtaaagg gtctggattt ttacctggac gattttagta ttcaggaaat acgaaaaatt
    29101 gtcacagata aaaattagat cttaatctta atcagaaaat caatcgctgc atgaaccgaa
    29161 ggaatatctg ttattgtcag aaccaaccgg tttttcctat cgagaagttt gcattttgaa
    29221 gagtgtgatt gtacaaagtc aattattttc ccaaaagtat cagattggaa ataggtttct
    29281 ttattgggtg agataaagtg acatttgagt tcggaattct tcaaaactat cttttcaaat
    29341 ccaagacttt cacccaacca tcgtagtttt acggtttcaa tcaattcacc cacgctgtcc
    29401 ggtaattttc caaaacgatc actaatggag tccgtgaaag cctttaattc atcctctgtt
    29461 ttgatattat ccaatttcga atacaaactg agccgctcag aaatattttc tacataaaaa
    29521 tcaggaatga gaacttccag gtctgtttca ataatgcagt cctgaaccag gtactgaaga
    29581 ttttgatttg gcttgataaa caaatccttg aattctgttt cctttaattc ctggatagtt
    29641 tcatcaagca gtttatgata cgtttcaaat ccgaggtcat tgataaaacc gctttgttca
    29701 gaacccagca aattcccggc tccgcggatg tccaaatcac gcattgccac cttgaacccg
    29761 tcaccaagtt ccgagaattc ctccaatgca gtgagtcttt ttcgtgcatc ggaagaaagc
    29821 atgaaggaag gaggagttaa gaggtagcaa aatgcttttc gattggaacg gcccaccctg
    29881 ccccgcatct ggtgtaagtc actcatcccg aacatatgag catgactgat gataatggta
    29941 tttgcattgg gaatatccag gccagattct ataatatttg ttgaaacgag gatatcataa
    30001 tagccttcaa tgaacctgag catggttttc tccaacttat ctccaaccat ttggccatga
    30061 gccactccaa ttcgagcatc cggtacaaga cgcaacacaa tatttgcaac ttcatcaata
    30121 tcacttattc tattgtgaac aaagaaaacc tgacctccgc gttgcaattc aaaactaatt
    30181 gcatccctga taactgtttc attaaattcg tgaagttcgg tagtaaccgg ctgccggttg
    30241 gccgggggtg tactgataat acttaaatcc cgtgcaccca tcagactaaa atgcaaagtt
    30301 cgggggatag gtgtcgcagt aagagtaagc acgtcaacat ttacttttat ttccttgagt
    30361 cgctccttaa ccttcacacc aaacttttgt tcctcatcaa taacaagtaa acccaggtct
    30421 ttgaacctga catccttatt tacaatccgg tgagtgccga taagaatatt gattttacca
    30481 gaatcaacat cctgaagaat ctctttgact ttcctggcgg gcttgaatct gttaacgtat
    30541 tcaattccga caggaaaatt tgccagcctt tcagtaaagg tacgatagtg ctgcatggca
    30601 agaatagtag ttggaaccag tacagcagcc tgcttaccat cacaaactgc tttaaaagca
    30661 gcgcgaattg ccacttctgt tttaccgaag cccacatcgc cgcaaactag tctatccatt
    30721 ggatgagctt cctgcatatc aacttttaca tcctcagttg ctttagcctg gtcgggagtg
    30781 tcctcgtaaa taaaggatga ttcaagctcg gcctggagga aactgtctgc tgaaaaagca
    30841 aaaccttcag actgttttct ttttgcatat agcttaatca agtccgaagc gatatcaata
    30901 accttcttct taattctttt ctttttgtta tcccattcgg ccgatcccag cttactcatt
    30961 actggaggca caccttcttt tccggaatat tttgaaattt tatgtaatga atgaacactg
    31021 aggaagagca aatcatcatc acgataaata agccgaattg attcctgttt cctgccattc
    31081 atctccactg tttccattcc cacaaatctg ccaattccat aatctatatg tgtaacatag
    31141 tcgccgggat gaagattttg gagctctttc aaagtaaggg ccctggattt ggtaaactga
    31201 tcgcgaagcc ggtaacgatg gaatcgttcg aaaacttcat ggtccgtgta aatcgcctga
    31261 cctaaatctt tatctataaa gcctttatgt atgctgatgt ttaactcttc tgctttcaaa
    31321 tctggattta tctcttcgaa gatggtcatc agacgctcca gttgttttgg tgattctgcg
    31381 gcgataatat tgcgaatacc tgcgcgctga tttgcctcta actcccgggc cattaattca
    31441 aaattcttgt taaaggaagg ctgaggatgt gtattgaatt gcagcaagaa acttcttctg
    31501 aaatgaaatc ttgagccaaa ctccactact cttaattcag aaaggcattt cagaaagctt
    31561 tctttatttt cgaaaagtgt ttccggactc agaactacct gagtatttcc ggccgattcg
    31621 aggatagaat tgaactcgac cgagcactta gcgaaattgg attcgatgat gtctaatgtc
    31681 attttcaaat catcaaacca gatactggaa ttggctggaa gaaaatgaag gaatggttga
    31741 cgctcttcat gcaggagttt ggtctgcaca ttgggaatca ggctgaggga attcaattta
    31801 tcaatggaaa gctgactttc aggattgaaa gttcgaatgc tttcaaccga gtcaccaaag
    31861 aactctacgc ggtaaggtat ttcgtttgaa aaagaaaaaa tatcaacaat cccaccccga
    31921 actgcaaact gacctgcttc ataaacaaaa tctgcttttt caaaatcata atttatgaga
    31981 acgcttatca aaaactcctg atcgatccgg tctccaactg aaatgggata agtattttta
    32041 ctaagggacc ttttatttac aaccttttca ccgagagcct cagaataggt aattatgaca
    32101 gttggttcag atttatcatt atttaaccaa gtcagcactt ctgaccggag caagacattg
    32161 gcattttcaa tttcttcata ttggtaaggt cttttataac tggccggaaa aaataaaacc
    32221 ttttcgttac caataatctc tctaacatca ttgaagaaat aggcggcatc ttccttatcg
    32281 gccaatacga ctaaagaagt tgatttatca gtgctaaata aggaagccag gattacagca
    32341 gatatacttc ccgagaggcc tttaagctga attatttttt cagtatgaaa attctccgct
    32401 aaggttttta taattccgtc ttcagaatac gatcgtatca gatcttctcg cgtcattgtt
    32461 tttcaactcg ggaccgcaaa agtaaacata tctaaatctt cataactaaa ttattctcct
    32521 ttaataatga atctgaagat ttagtattta ttattttgct gttatgaaaa aattagcctt
    32581 tggagccctg aatttgtacc gaaaactcaa attattcagg ttaaaactga aaggaatgac
    32641 aattgaggat gagcatgagg aaagaattgt ttccgggaaa gcatgggaag aattttgcga
    32701 aaacctgaaa cctgcaggtg ctgcccttct ttacggggac tcaccgaagg atccattcag
    32761 ccaggcagag ggataccggt atcttaccag gttggtaaga gcatccctgg aagctttcgt
    32821 tgaattttct gatcctgaat acccggtatt acgaagaatg gtgcatgaga caataaagat
    32881 gggtgccgat aaccctgata attattatca aaacactaaa attaacggcg aatatgaata
    32941 ccggatttat ggaatgcggg ggacagtaca ttatctcggg tttggaaccc agcgcggaga
    33001 ttatggtaaa acaggaaata tggaaccaag cggctatctt gaaggatctg aattggagat
    33061 aaatcctgat ggaacttttg aaatccacgt aagttgtgaa aagaaagaag gaaactggct
    33121 gcctttgaca aaggattcca gtattttgat ggtcaggcaa acttttttgg acagaaaatc
    33181 tgaggtgctt gcagatttgc aaattgaaag ggccggaggg agcaagaagc catcaatggt
    33241 aacaccaaaa acaattgatg atgggctaaa tgcggcagca gcttttgtat caggagccag
    33301 tctgttattt tcacggtggt cggatggatt taagaagaat aagaataaac tgcccaggtt
    33361 tgatcaggca aaaagtaatg ctgcaggagg tgataataat attgcctact accacagtta
    33421 ctgggaatta gcagatgatg aagcgctggt tatagaaact acgccaccgg aatgtgaaca
    33481 ttggaatttt caacttaata attactggat ggaatcactt gattaccggt attatacaat
    33541 tcacataaat aaacaatccg ccgtttacaa taccgatgga agtgtaagga taatagtagc
    33601 acataaagat cccggattgc caaactggat aaacactgtg ggtcataacc tgggcacgat
    33661 gtgttttcgg tgggtgaaag caaaagaaaa tcccgagcct gaaactaagg ttgtaaaatt
    33721 tgatcaagtg gtgaaacttt aatcaggtgt cagaagatgt aggtttaaaa tttgaaaaga
    33781 actatccggt acccatacgg atctggaatt tttttggaaa gaccggcctg caaagaaaaa
    33841 ttaagactga cgatctcctg aagaaagcaa aaagaaacac cagattggag gattttggca
    33901 atgaattttt tatggagcct ttggaagtct tggttaaatc gataaatgaa gaagcagaat
    33961 tgcatgcttt cggaaggtat gttacaaagg caagactagt aaatctactc tctaacaggt
    34021 tgagagcgga agattacctg aaaagaaatc cgcaaattaa tgatatagaa ttattgccaa
    34081 tctggtggat atcaggctta caaagaaccg gaaccacctt tttgcatcgc ttactggcca
    34141 cagttccggg caccgcgacg attaaaagct gggaagcttt gaatccggta ccgctggaga
    34201 aagactttca aaataaaaaa agaattgcaa tggcccagac cagcgagaaa ggacttcgct
    34261 atatcgctcc ggatttcttt gccatccatc cggttgagca tttatctccg gaagaagaaa
    34321 tcttactgct cgacatttct ttcatgagca cagtcccgga agcaacgttg cgtgtgccca
    34381 cttatgcaaa atggctggaa aagcaggatc agacgccggc ttacgactat atgcaaaaac
    34441 tgatcaggat tattcaattt caaaaaggag taaaaaaaag atgggtgctt aaatccccgc
    34501 atcacctgga atacctggat actcttacta atgtgtttcc cggaactaat ttcattttta
    34561 ctcatagaga tccgttgcaa accattgcct ccttttgcag catgatctac ttttcaagga
    34621 gaattttttc aagtaatgtt gatgaaatgg aaattggaca ccattggcta agaaaaataa
    34681 aattaatgct ggagaagagt atgaaattta gagcaatgaa caaagacgtt gagtgtgcag
    34741 atatttctta ccaaaaccta atcaaagatc catttggcca ggttaagatc atcctggatc
    34801 gttataataa tactgtaaca gaaacagacc ggagagcaat tcaacaatcg gtgaagcaaa
    34861 ataaaagaga taaatttggt ttacataact acgaactgca ggattttgga ctgacgggga
    34921 atgccttaga agtcgagttc ttagaataca aaacaaaata ctcacaatac ttataggatg
    34981 gcggattacc aaagcccggt tactgcaaca cttacaggca tccttaatgc ctttaagaaa
    35041 caaaaaagga taggggattt aaaagaaacc gaccgggtgg atgggaaaac ctgtcttatc
    35101 acaggggcta attccgggct tggttttgct ctcgcggttg agctggcaaa acgtggagca
    35161 aaagtattaa tggcttgcag aagcggaatt cctgacgccg gtgaaaaagt gaagaagtat
    35221 tctacttcag aaaaagtgga aatgtttaaa gttgatttat ctgatctcac ttctgttgat
    35281 gcattggtta atgagttgaa atcaggaatg ataaagattg acattttaat atgcaatgcg
    35341 gcaacggttc cctccaaagc aaggaagaca ccccagggat ttgaaaccat gtttgtagta
    35401 aattacctgg ccaagtttgt tttattgaac agaatccgga aggaaaattt attgagttcg
    35461 gataatacgc cccgaatcat aattatttca tcagaatcgc atcgatctcc aaaaaatctg
    35521 gaatttgagt cctttggtaa ctatgaagat tttgccatgt caaaagttgt ggcactttat
    35581 ggatactata aactggcgct gacaacactg gcgagagaat tttccagaag gacaaacgac
    35641 aaaaagttga atgtttcagt gcatgtaatt tgccccgggg ctataaacag taatattgcc
    35701 agggaagccc caggttacac gaaaccccta atcaaattta tttttgcttt attttttcag
    35761 aaacctgaga aggctgcgaa accagtttct tatttggcgt gttcaaaaga aattgaaggg
    35821 agaaacgata tttacctgca tatgtatacc gagaagttac ctgatgcgaa aactatggat
    35881 acaacaaatg gaaaagcctt gtgggatcgc tccaatttct tgctgaaatc cgttaattac
    35941 ggcagaactt tttaatttta agactctttg aatttttaac tccatgtatt atctgaggat
    36001 ggcgggtgtt tttgtaatta tttatgtggc tatttgcctg atttactatt tcattcagga
    36061 actgctgatt tttcatccca aaaaattaca gcaggacttc gagttttcgt atggagacaa
    36121 ggctgaggaa atttttctgg aaaatggcag cgccaccatt cacggtttat attttcctgt
    36181 tgaaaattcc aaaggactaa tcctttactt ccatggaaat agcggcgctt tggatggatg
    36241 gggctacctg gggaataaat tcaactcgat tgagtactcc gttttcgttt cagattaccg
    36301 cggctatgga aaaagtaccg gcaggctttc agaaaaaaat ctttattcgg acgcacaggt
    36361 tatttatgat tattttaccg gtagatgtga agacgaaaga attattatta tgggaagatc
    36421 actgggttca gctattgcca ttgaattagc attgcagaac agctatgaaa aattgatatt
    36481 ggagtctccg tttcacagcc tcacaggaat gataaaaatg ttcttcaatt atcttccaca
    36541 tgacttgttg ttgcaataca aattcgattc acaaagcaag attgataaaa taccggaacc
    36601 tatattgatt ttccacggaa ctaatgatga agttgttccg atagaatcgg cgcggaaatt
    36661 ggaaagccgg attcaatcta aatatcataa atttgtagaa atagaagggg gcgggcacaa
    36721 taacctttct aatttcgacg tctacaatga agagttgata aaatttttag acaagtgaat
    36781 ttttataaat tgataaaagc agcgggaatt ttgtttttgt tgtcgcatcc aacaattgct
    36841 caggatccac agaatatgaa agttgaaaac caggtgctgg attgcgggaa aatgaaaacg
    36901 tcttttgcag acattaaaga ggcaaaggaa actttagaaa aaactgtttt tgcgtttacc
    36961 caggaactaa aaacaacaaa aagaaacgga atcaaggaag tgtattatta ctcttgcgac
    37021 aatgaaacag gcttcctatt aataaaagag gatgatagag aaatagtata tagagattac
    37081 ccgcgttttc agtggaagct tttaatagag gcaactgacc tggatggctt ttatgataag
    37141 aatgtgcgag ggaagtatat gttggtggtg agggaccagt agagacatta acctcgccaa
    37201 ttaactcatg ccagtaataa taaccttgca gtaaggaata gtaaattcta cctataaata
    37261 tcattgagca ctcccgccag tcgaactccg gattccaata atcgtctttc cacaacatgc
    37321 catttcctgt acacgtattc atagcttagg ctgccgtggt ctggcaaatc ataaaccagc
    37381 ggccgaagtt cgcgggattc attggcccaa tccaatacag aggcagattg caaaactgca
    37441 acttcctggg gggtgacctt atccagttcg tttgccaatt cggtatagct gaagttcttt
    37501 ccatcaatca tctggctatc ccaaacgtta tgaagattag tagaagttcc aaagtatttt
    37561 acccgggcat cgtttccccc ctgatcttca ccattcccca cgtgtaatgg ctggtgaata
    37621 tctccaacca aatgtataag catcttcaca tgttcagact cagtcttttt atccagtgtg
    37681 tgagttttta gttccctgat tagcctggtg atggcttcaa tgatatcacc atcaggattc
    37741 ttttcaacct cagcatagga aacttcatcg ggtaaagtta cccagtgcca gtcatgagta
    37801 tgatcatata aagaatctga ccggatgtca tccatccaat tacttacata cgcgagtgat
    37861 ttaccatcca gaattctgtc aatttcattt tttgcttttt ttgtcagatg attttctgca
    37921 atttgtccaa ctgttctatg tccggtcacc ccccagccaa taacctgcgt aaaacacatt
    37981 accaataaac cagcaaatac ctttttcatc ttctcatgat ttcataaaac gttttatctc
    38041 gtttgccaat gattccatat caactttttc aatggaatga gatgtgtttg ggataaccag
    38101 cagttcaccg ttttgcaacc aaccgtatac ctttacggtg tcatccactt tcgccatcgt
    38161 atctttatcg cccacggcaa gtaataccct gcactttaat tgctgataga tctccatggt
    38221 taggggggca tttgtaccca tcgattctat aaaagcgctg gtcttgttaa ccagactggc
    38281 ccagtgtgtt agtccatgca tttgctttag ccggtttgca aattgaggag ctttttcttc
    38341 cattctcccg gggtttagaa atgcgctctc cttttgtgat tcttcctctg tccaaagcaa
    38401 ctttgtacct aaagtaaata tcttgttgac tttatccgga tatctatggg caaataatag
    38461 cgccgcatac ccgcccatgc tgtacccaaa caaatttacc ttgctaagtg acatacgccc
    38521 ggcccaatta tagatgtctt ccacaaagac atcaaaggaa aaatccttta taaaaagaga
    38581 agggttggca tgcccggaaa aatccataag tccgggagag aattcatttt caagaaataa
    38641 agacaacggg gcaacctgcg aggaagaacc cagagcgcca tgaagcaaaa gcaggtcggg
    38701 tttagacatt ttgaattaaa tttatcaaag atattatcaa attcgcttat gagcggacga
    38761 tactccatag tttcgtcacc caaatccatt gaaacgcact tcaaggcaaa gtttgaatat
    38821 gcatttgaag cgaatgccaa tgcccatccc atgcagatgc ttcctgtgat tagcagtttg
    38881 catccggata aaatcgttcc ttattattgg ggattgattc catactgggc taagagctct
    38941 gatattggac agaagaattt taatgcatgg gcggagggaa ttcataagaa ttcttctttc
    39001 aagattcccc tggaaagacg aaggtgtctg gttcctgcca atggctatat tgaatggcga
    39061 aatttcagcg ggattaaaag tccatatttt gtttatagcc cgggaaatcc gttaatagct
    39121 ctcgcgggac tattcgatac atggatgaat gaagccacgg aggaaattac ccggtctttt
    39181 acgattatta caacaaaatc taataaccgc ctgagagtta tttcgaggag gatgcccgtt
    39241 atcttaagag aatcaaatta tcaaaaatgg ctggatgttt caaaagatta ccggcaggcg
    39301 atgagtttgt tgcgaccaat acccgacaaa ctaaccaatg cttaccacat atcactgagc
    39361 atcaataacc ccgtccataa ttacccggaa ttactggaac cacagcaaga tccattggct
    39421 aaagataatc tggacataaa aatgtttttt gtctagaagt cgtcaatcaa ctcctcaatt
    39481 tctcctaatt ttttatcgct aaaggcaccg gaagtcgcat aaatgatctt tccatcctta
    39541 tcgagaacaa aaaaataagg cacatcttta tgttcgaaat caagcgcttc tttataggtc
    39601 ttaagttcgc ccttatagaa aagcatatga gggtataagc tttcatcaag ttctttcagt
    39661 gcctgtcttt tcgctgttcc ctgggcagca gcttttaccc ccgtaaacat gggcacaaag
    39721 taaacattga tgtcataagt gaaatcggca aacaaaccgg catcgtcagc gcttcgaacg
    39781 aatttattat agactggagt aaaccaggta ttcaaatcct cttccgattt tttggaataa
    39841 gcgagaccaa ccaaagtaaa ttttcctttg gtatcatcgg ggatggttac ttttttgttt
    39901 tcaacagttt cagcagttag ctcgggaaag gaatcaccaa cctgggcggc cgaaattgtt
    39961 gcgcagaata taaggagaag aaaagcgttt gagatctttt tcattgcaga ttggttttgt
    40021 tttgaaacga aacctgatat tatttagtac ccgttttggg ctgctatttc agctaatctt
    40081 tgttgaattt cactttccgg catccatcta ccacgaacca ttacaccaat tcggcttgaa
    40141 atcgcggaaa tgtcatccaa tggatttgca tttaacagga tgagatcagc agaatgcccc
    40201 gctttaacca ttccaaattc gttttccttg ccaaaaaaaa tggccggatt gagtgtccct
    40261 gatttcagag catcataatt gggaatgcct gcatctacca cggctttcaa ttcatgttga
    40321 attgaaaagc cgggaacgtt aaaaatctga ggcgcgtcgg atccaagtag catccctaca
    40381 ccggcattat ataagctttt taaaattttg tctctaagct caatgaatgc aatcgcattt
    40441 tcaactgtgt acgcggcgtt agactggaat tgcttcacat tattaatcca tgcttccagg
    40501 gtttgccggg gcatatattt catttccggc tctgcaccaa tttcatccgg agataatgac
    40561 cctgcccagc gtttcattaa tgcatgggtc ggcacaaccc acacattttt tgcagccgtg
    40621 cttactgaga ggtcatccaa aagagataag tcagcatatt ttacaaagtt gaatccaaaa
    40681 aatccatttt gggtagggtc aacattcgcg gattcaggga ccaacccttc gagataacca
    40741 tcaaggtgat cgacagagcc atactgagct tcaatagctt ttcggattcc tacatgtaca
    40801 gacacatgtc cgctgaacgg aatgccaact tcattagcgg aggcaacaag ttcattaaaa
    40861 acttcaagtt gaatacccgg atgaagcttt agaaaatcat atccggcctc cttttgttca
    40921 gttaccatct tttgggcgtc ctcaggtgtt tttacagtat ttccattaag agaaggcccg
    40981 gaagtgaaaa ttcttggact taaaatctcg ttattggctg ctttttccct gagctcaaga
    41041 tgtgatggtt gacccagcat gccccggatg gtagttacac catttgacaa ataaaggaat
    41101 agcgtatttt ccaggtactc cgggcttgca ttagttccgg gaatatgcgc atgcatttca
    41161 gccaggccag gaataagaaa ttttccggtt ccgtctatca tggttgcttg ttccggaacc
    41221 tttatagaag tggcatcacc aatagcagta atttttcctt ccgaaattaa aacagtttga
    41281 ttttccagaa catgattttc atccatgggt atgacattca cattagtgaa tgctgtaacc
    41341 ggagccaggt tttcattttg tgattccccg ggaggattac aggataataa taataacaga
    41401 acaaggtaag gaccaattct tttcatagcc gtgtagttta taatgaagct aagtaaatta
    41461 ttcaggagtt aaaaagagaa tttagacaaa tggtcacatt ctctctaatt ctatcaattc
    41521 gtaatcctgc ccttttccaa attgaatcag gtcactgaaa gtataggttt cgagagaatt
    41581 taaatctttc agtttaaaat catgatcact taaaatgcga gtaacctgaa aaattatttc
    41641 ttcaccaaaa tttcggaaaa aatacttttt tcccgttctt atcgtattta aagcaatgga
    41701 catgttacaa atttaaattt taaatttgta aaagatggaa gggaagaaaa tggttttgtt
    41761 ggcattaatg atgaccccgg gtttctatgg caacgcccag gaaatcaaaa taattgaaaa
    41821 ccattccgat ctgcagcctc atcctttagc tatcattaat agtgaattcc gtgaaataaa
    41881 tcttagcata tctcctgatg gaaaatacct ttatttcatg agtcttcgcg gtggaaatcc
    41941 ctggtctgtg gaaggttatt ctattttcag agggaagtca aggtatgatg gtgatatctg
    42001 gtattcccaa aaagaagccg ggcaatggac tgatccgatt tgtcttcctc caccggtcaa
    42061 tacttcaagt gctgaggatg agcccaatat tagtccggat gggaattccg tcatgtacca
    42121 gtcgtggaaa gattcgtggc tgactgatgg aggtccctat tacattagca agcttaatgg
    42181 agacaaatgg gaaaatccgg agggaatgag tggcggcatc aatcaatact tcaaaataaa
    42241 tttcagggat tatgaaggtt atgccacaga tggagcctca ctctctccag atggaaatat
    42301 ttttatggtt gccctggcac ctgattacaa tgattccatg gatatctaca tctcgcgaaa
    42361 ggataatggc atctggactt atctgaaaaa taccgcgctg agtacgtctg gagatgagcg
    42421 atcaattttt attgcaggag acaacaaaac agtttatttc gcttcaaacg gatatgccgg
    42481 attcggagga atggatattt ttaaaacgat tttaaacgat gatgattctt ttggtgaaat
    42541 tcaaaatatt ggaaagccat tcaattccga aaaagatgac caaggcttta ttgtcacagc
    42601 ttcaggggca gagtcctact ttgtaagaga tgatgacatt tattattgcg acctgcgatt
    42661 ggcagcagat gaactaaaac cgcttccttc gatcatcatc agcggagttg tgacagatat
    42721 ttttggatac ccccttgaat cagaaattac aatttcaaag aaagaaaccg gccaactgaa
    42781 aggaaaatca acatcaaatt ccaagaccgg tgaatacgcg gtctcaatcc taaaggaaga
    42841 aggagtattc gaaaaaaaag taaaggcgaa tggtcacaaa attcaaaaag agaattttac
    42901 gctggttgat atgggccggt ttgaagaaaa aactataaac acggtgctgg ttgaggaaaa
    42961 tacgctattg atccattttg attttgatga agttagttta tctgaatatg acagatcatt
    43021 gctagacagt ctggtagctg agatgtaccg taatcgcagg ttgcggctga tgatggaggg
    43081 acacacagac gagcaaggaa cagcggaata caattcatcc ctttcgaggg aaagggtaga
    43141 ggaagtggcc aggtatttga gagagaaggg catccctgaa ggaataatga aattaaaggc
    43201 atttggagaa acaagtcctc gtgcgggact tactgttgta aaccgggacg cactaaaccg
    43261 cagggtagag ataaaggtta tggaattgaa aagataaatt atatttcttc agataaggat
    43321 agggaaagca tcctcattct attaattttg acacctcaat agcaaaaaca agaatgaata
    43381 taaagggtaa acttctggag atatttgatg tggttcaggt tacggacaca tttaagaaaa
    43441 gagaattcgt gttggaatat atggagaatc cccaatatcc cgaatttgtg aagtttgaat
    43501 taacgcagga ccgctgtgaa attcttaatg cattcaaggc aggagaagaa gttgaagttt
    43561 ggtttaatct ccgtggaaga aaatggacag atcccaaagg agaagtaaaa tattttaatt
    43621 cactgcaagc ctggagaatt caacaggcag gcgcaggtca acaagaggat tccccccgga
    43681 aacaggaaga gccaggatgg ctggctggtg aacaagacga tcttcctttc taaataaaac
    43741 aattaaatga aaaaatggat ttggtctggg gcaatcgtta ttctaataac ggcggttgca
    43801 attattgccc gtcccattct gctaattctt agtggctttg gcgcaaaaaa tctgtgttcc
    43861 tgtgtttata attcaggcag ggaaccttct tcagttttaa aaaacgaatt gggaaacttc
    43921 ccattaactc ttggtaactt ttatcttgat aaaaaggatt cttctgcaac cggatcagtt
    43981 ttaggttttg ccaaaaggac tgcggtttat aaaaatggtg aaggatgtac tttgctggtg
    44041 gattattcca aagaggaatt caaacagcgg aagccaatgc cgatagagtt atcggcggtg
    44101 gaaatcgata ctttaccatg gccttttgga acccaattcc cggatacggt gataccaggt
    44161 gttaactatt caaaactgag tgcgataatg gatgatgcct tcacagaagt tgaaggagaa
    44221 aggaaaaaga atacccgtgg agtggtaatc gtatacgatg gtcaaatgat tctggaaaga
    44281 tattctgaag aatttgatcc tgaaacacca caaatgggct ggtccatgtc aaaaagtatt
    44341 attaatgcgc tggttggtat tcttgtcagt caagagaaac ttgatgttta ttctcccgcc
    44401 cccgttccgg agtggagtga tcgagcagac cctaggcatt taatatccat tgatcaatta
    44461 ctgcgcatga gcagcggaat agagtgggag gaaaactacg gcgctccaag ttcagcaacc
    44521 aacatgctat ttaccagcgg agacatgggt aaatatacgg catcattgcc tttggaagca
    44581 cctcccgatt ctctgtggaa atattcatcc ggctcctcaa acctgttatc cagaataatt
    44641 aaacaaataa cggatgaaga ttacctgagt tttgttgaca gagaattatt ttcaaaactc
    44701 ggcataaaat ccattgtttg ggaaaaagat gaaagcggaa ctttggttgg ctccagtttt
    44761 atgtgggcaa gcccccggga ttgggcgaag cttggattat tgttcttgaa tgatggagtt
    44821 tggaatggcg aaagaatcct tcctgaagga tgggttgaat actcctctac accaacaccc
    44881 aaagcaaccc gtggctatgg agctcatttc tggctaaatc gaatgcaaga aagagaaaat
    44941 acccagcatc ctgatgctcc cgctgatatg ttcagcatgc aggggtttga agatcagaga
    45001 gtctttatta ttccatcaag aaaattagta attgtcaggt taggccaaac ctccgacgag
    45061 cattttggat tcaatgcgtt cttaaagaat attcttgagt gtttccccga taggtaaact
    45121 atttatttct gggtaagttt gacaaccatg ttcaaccaac tagggctata tacggatttt
    45181 tatgagctta caatggctca gggttttttc aaaaatggct gggccaggaa gaaagccggc
    45241 tttgattatt tttttcgatc cctgcctttt gaaggaggtt atcttatttt tgccggtctc
    45301 tcggatcttc tggagatgct taataggtac cggttttcca aagatgattt gaaatacctg
    45361 aggtcaaagg gatttgaaaa accatttctg aattatctga aggattttag atttcaggga
    45421 aatatttatt catgccggga aggcgagatc gtttttccaa atgaaataat tgtgcgggtt
    45481 gagggtaata tcatcgaaac acaattgatt gaaacattac tgcttaacat tctaaatttt
    45541 gaatctctaa ttgccaccaa agcatccagg gtttgttatg cggccaaagg aagaaaagtt
    45601 attgatttcg gattacgtcg tgcccaggga tttggaggtt ttcatgccag caaggcagca
    45661 gctatcggag gagtaacagt cacttccaat gtgatgtctg ctaatgaatt tgaattagaa
    45721 gtttccggaa ctcaggccca ttcgtggatc gaagcgtttg aggacgaact aaccgcgttc
    45781 cgaaaatttg ccgaattgta ccctgataat tgcgtgctgt tggttgatac ctataatacg
    45841 ttgaaaagtg gagtacccaa tgcaataaaa atcgcaaatg aaatgaaaag tcgcggccgg
    45901 gaactgaaag cgatccggct ggatagcgga gaccttgcct atctatccaa gaaatcaaga
    45961 gaaatgttag ataaggaagg gtttaagaat gtgaaaataa ttgtttcgaa ccagttggat
    46021 gaacacatca tcagaagttt gttggctcaa aacgcgcaag tagatatttt tggagtggga
    46081 acaaatttaa ttaccggcaa ggaagacgct gcattggacg gtgtttataa attatcattt
    46141 tttgatcgcc tggataggtt gaaaatttct gataatattg aaaaaactct tctgccggga
    46201 aagaagaaag tattaaggca tttagataaa gggggtaact tttacgctga tgtgattgtt
    46261 tctgatgacc acaccaaacc atcagtaatg attcaccctt tttctccaga gaagagatta
    46321 aaattaacgg gctttgcaca cgaaaatctt ttgattccgg taatgaaaaa gggaaaatct
    46381 acgctgaaag aaaatcacgt ttcacgaata gctgaatatg catccaaaag actttcccaa
    46441 ctacccgagg aacataaacg ttttgagaat cctcatattt acaaggtagg tgtaagcaat
    46501 aacctcttga aaaagaggaa taaaatgata aaagatataa gatcgaagtt tgagtaagca
    46561 gataatatga aagctttact aattgtggat gttcagaatg atttttgtcc cggcggaagt
    46621 ttggcagttc cggaagggga taaagttgta aatgttatta attcccttat ggagaaattc
    46681 aaaatagtga ttgcaagtaa agactggcat ccggagaaga ctgttcattt tgaaaagtgg
    46741 cccgttcact gcgttcaaaa tacgaaagga gcagaatttc accccaactt aaataccgtg
    46801 ggaatccaga atatttttct gaaaggaact ggcaacaaag atgacggtta ctctgctttt
    46861 gaagcaacaa atgaaaatct tgaggatttt ctgaaatctc atggaattaa agagttgtac
    46921 gttactggtc tggctacaga ttattgtgta aaagcatctg ctctcgattc atcaaaactc
    46981 ggcataaaaa ccattgtggt ggaagatgct attaaaggag ttgaagttaa ccctggagat
    47041 gttgcgaaag ctttaactga aatgaagaat caaaatattg aatttattca atcaaaggag
    47101 atctgacgcc ggataaaatt aatacgaaag tttaaggctc aattctaagt tttcctaatt
    47161 ttatcatgaa cgacacaaaa ggaaaaccaa aagttccgtt agcataaaat tcgtggttat
    47221 ttatcaaggt gaaatcaaat gcgattctcc gggtcattaa tcgcattcca taagaaatta
    47281 tataatagtc ggggatcaac cagttttctg aaatgagcgc aatccgggaa cccaacctat
    47341 agactccact taggcttatt gttggcctgt ttgtgggttt ggaatttaat aacccataac
    47401 cggtaattaa cgtaatgttc cggtccgagt ttcctaaagt tgcacttcca taaataactc
    47461 ccagatcata atactttgaa acatccacgt tcgtgccgtt tatttcaagt gatggcgctg
    47521 atagaaacag aatacctgaa cccagatgaa gattttccct aaggggaatt gaaatcctgg
    47581 gattaagata atagatggga tggcctaaag ccgcagtgag aagttcaaat ccgccaccca
    47641 ctgaaacggc attcgtaata ccgtaattga gtgtgttgat aacaataaaa ttattttgat
    47701 aattaaactc attaggttca aggggaagtc cgctgggtcc taaaacataa gtaccctttt
    47761 tgggcgaagc aaaccatttt tcattggagt cacttgtttt ttcaggccca cctttaattc
    47821 ttctgatgtt tttaacatta actctgaaat caccggcatc atttctcaaa gttacaaccc
    47881 cttcgctgaa ttgcatcaca ataccggtaa gggtcgtccc atcatttaag atgagcgtat
    47941 attccgcacc gggctcaaac aaaggctgga aatcctgagc ccgtgactca aaaagtagca
    48001 aaaggtaaaa gcagagaaga aatctgaagt gcatgaattt gggttatgcc aaattcaaat
    48061 ttagcaaagt agggaaagtt ttatctgaaa taactaatta tagttttgac ttttattcaa
    48121 aataatttcc tgaacctgtc tgcccaattc ctcattccgt gtattggcat agatggcggc
    48181 aaatttacct attagctgat cagcaaattt cagcatggcg gtatccgtgt gcctgatgcc
    48241 cgtctttgtt tgcctgaatg cttcctccac ggcgtttagg aagctttcgt aacgtaattg
    48301 ctccattctt tgcaaatttt ccagagtaat agcagacctg gttttcatag tttttccttt
    48361 tattcttatt aaaataaaga ccttattttg acaatttgtg tcaatttacg taaaattgaa
    48421 aatggtagct caggctaaaa tccttagagt attaaaactg atttcaattc tgaaatccag
    48481 aagcaaaacc atcaaacaaa tagcttcaac tctggagata accgaacggt ctgtttaccg
    48541 gtaccttgac ttattaacag aagtgggttt tcttgttgat aaggattttg aagataggta
    48601 ttttattcac gtggaagaag acgaaaaatc aaactttaat ttttcaacgg aagaatcctc
    48661 gttactcaaa gaattgattt ctgccggcgc taccaatcac ccacttaagg aaacactatt
    48721 gaagaaacta tatttgaatt cagatttgaa atccatttcc aggaatcttg aaaaagccag
    48781 attgggaatg ctggttggta aaattaatga aggtataaca ttgcaaaaac aagtagtcct
    48841 gaagagttat cattctgcaa atagcaatga aattaaagac cgtttggttg aacccattac
    48901 cttgggtgaa ttgtacgatg aggtgattgc atttgacact tcagacagaa aagtgaaaca
    48961 ttttaaatta gagcgaataa gtagcgtaat tattttgagc aaaagtcaaa gaaacacctc
    49021 aagtcatgcc caggcaaaat ccgatatttt tgggattgat ggaacctcgg aaacatggat
    49081 aaagctcagg ctaaatctcc ggagttattt gttacttaga gaagaatttc caaaatccat
    49141 tccttacctg aaaaaggagg aggaatatct atttaatggt ccggtccgga gtttcaacgg
    49201 tattgggaga tttgttctcg gtttaatgga ttcggtggaa atcgtgggac cacccaaatt
    49261 caaaaattac attaaagaaa agtgcaaatt aatggtggag tccttataac tatcaatagg
    49321 aatcgaccaa tcagaaagtc agtttgccat tcagagaaag aattaatcag attgtgaaat
    49381 gcattaatta aattcaattc tgaatttaga tcaactcact aaaacaataa acatgaaaac
    49441 gttaaagcaa gtttttgcca tcatctttct tctgccaggt ttccttatgg cccaggagaa
    49501 ggcagatctt aatgccgttt ataaaattaa acaggaaggc cttgacaata gcaaagtgat
    49561 ggaaatagcc cggcacctta cagatgtaag cggacccaga ctcactaatt ctcccggtct
    49621 taagcgcgcg caggaatggg cggttgagga aatgaaaggt tggggacttc aaaattctgg
    49681 tttggaaaaa tggggggatt ttggcagggg gtgggaagtt caaaaaaact atgtagccat
    49741 gacgtctcca tattaccttg cctttatagc atccccgaaa gcatggactc ccggaaccaa
    49801 tggattaatc tctggcgagg taatgatgct tgatgcatcc accgaaggag atctggaaaa
    49861 atacaaaggt cagctgaaag gaaaaattgt actttttgac acggatgccg acacaagtcc
    49921 ttcatttgaa gctgatgctt caagacttac cgcggaagat ttggcagagc gggctaaaca
    49981 acctattggc ggtggcc
//
LOCUS       DASWHX010000190         3136 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_201917, whole genome shotgun sequence.
ACCESSION   DASWHX010000190 DASWHX010000000
VERSION     DASWHX010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 3136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3136
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201917"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            399..2594
                     /locus_tag="VGA21_12690"
     CDS             399..2594
                     /locus_tag="VGA21_12690"
                     /inference="COORDINATES: protein motif:HMM:NF039587.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HEX9651413.1"
                     /translation="MKRTAFILLIVITLNYFSVAAYAQSGISINRAIDEIVLDGEMNE
                     ESWKNARLVTNFTQYYPTDSLIAEARTEIRLTYDDKFIYVYAWMEETKEGKYVTSTLR
                     RDYRGGASDGITIVFDTFKDQTNAFVFGVNPFNVQREALVANGGSQGDDFDLSWDNKW
                     YSQTGRGDGYWTAELAIPFKSIRFKNGVETWNVNFYRIDTRYNERSIWTPIPRNFEMF
                     TLAFTQKMQWDKPLEKTGPNISLIPYVSGGISKDHENLESQKETGNIGFDTKIGVTPG
                     LNLDLTVNPDFSQVEVDVQQTNLSRFELFFPERRQFFLENADLFSNYGFERSSPFFSR
                     RIGIVRDTINDINLQNPILFGARLSGKLNNNLRLGILNMQSAKDDEVGIPSLNYSVIT
                     LQQKVFRRSNVSAFFVNKQNFTEDYENDSTDFNRVFGIYYNLSSADAKWNGKLFYHQS
                     VNPYKQDHDFAHGLYLEYSVRTFGVEWAHEWVGENYDAQVGFVPRKGFLRLNPELSLR
                     FYPKSGIINNHGPRGELSYVWSEIYGKLDHELQGNYNISFQSGANASVDFTQNFIYLT
                     DPFDPTNMEGVELPAFSKFTYYDYRLSFRSNDRRAFYFNINHQGGEYFNGTRYGISGG
                     LNLRAQPYVLITANFNYNHINLPEPFSDANLFLFGPKLDVTFTRNLFLTTFFQFNNQS
                     ENVNINARLQWRFKPVSDVFLVYTDNYASTNWAVKNRAIVVKVTYWLNL"
     gene            2681..>3136
                     /locus_tag="VGA21_12695"
     CDS             2681..>3136
                     /locus_tag="VGA21_12695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009579045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF255 domain-containing protein"
                     /protein_id="HEX9651414.1"
                     /translation="MRKGIISVFSTLILVAVIVSAAFITSVPGIKDLSLKANNESVVK
                     WYTFEEAIEINKKAPRKIFVDVYTDWCGWCKKMDVNTFNNPTVAEYLNEKYYPVKFNA
                     EQRESIVFRDHTFKFIAQGARGYHELAAALLNNKLSYPTVVFLDEDFNMI"
BASE COUNT          971 a          582 c          632 g          951 t
ORIGIN      
        1 atgttacttt gccgccttct ttgagttcaa tctctgcttc acctaaccag gaaagtaatt
       61 tcggatctgt aaggcattgc catacctgct caataggata gtccaaaagc cgtttcttgc
      121 tgatgtccct tttcatattt aatttgtttt tttgaccagt acgaatttaa ataagtttag
      181 aaggaaaata aagggaatcc aatacatttc tgatgggctg ggatgtgctt tcaaatcagt
      241 tcgaattgga gacacggtga ggaatttgtt tcgaagaaaa actccaaccc tccttccaag
      301 gcttaatata taagttgaaa accgcttgaa cccccaattc gaatatttat aaaatttaca
      361 cttccttttt tggtgaagca cctatttttg cgcaacccat gaaaagaaca gccttcattt
      421 tacttatagt tattacacta aattattttt cagttgcggc atacgctcaa tctggaattt
      481 cgataaacag ggccatcgat gaaatagtat tggatggtga aatgaacgaa gaatcgtgga
      541 agaatgcccg gttggtaacc aactttaccc aatattatcc gacagattca ttgattgccg
      601 aagcccgcac agaaataagg ctcacgtatg atgataagtt tatatacgtg tacgcgtgga
      661 tggaagagac aaaagaaggg aaatacgtta cctcaaccct ccggcgcgat tacagaggcg
      721 gggcttcgga tggaattact atcgtattcg atacattcaa agatcaaacg aatgcatttg
      781 tttttggggt gaatcctttc aacgtgcagc gtgaagccct ggtggcaaac ggcggaagtc
      841 aaggggatga tttcgatctt tcatgggaca ataagtggta ttctcaaacc gggcgtggtg
      901 atggatattg gaccgcggaa ttagccatcc cttttaaatc cattcgtttt aaaaatgggg
      961 ttgaaacctg gaatgtaaat ttttaccgga ttgatacccg gtataatgag cgttccatct
     1021 ggactccaat tccaaggaat tttgaaatgt ttactctggc atttacacag aaaatgcaat
     1081 gggataaacc tcttgaaaaa accggaccca atatttccct tataccctat gtttccggtg
     1141 gtatcagcaa agaccatgaa aatctggaat cacaaaagga aaccggaaat atcggttttg
     1201 atactaaaat tggtgttact cccggtctaa acctggactt gacagtaaat ccggatttta
     1261 gccaggtaga agtggatgta caacaaacca atctgtcccg gttcgaatta ttctttccgg
     1321 aaaggcgaca gttttttctc gaaaatgcgg acctcttttc taactatggt tttgaaagat
     1381 catctccatt cttttccaga agaataggga ttgttcgcga cacaataaat gatattaatt
     1441 tacaaaatcc cattttgttc ggagcgcggc tgagcggtaa actcaataac aatttgcgcc
     1501 ttggaattct caacatgcaa agtgcgaaag atgatgaagt aggaataccc agtttaaatt
     1561 attcagttat tactctgcaa caaaaagtgt tcagacgatc aaatgtttca gccttcttcg
     1621 ttaataagca aaattttaca gaagattatg aaaatgacag tactgatttt aaccgggtat
     1681 ttggcattta ttataatttg tcttccgcgg atgcgaaatg gaatggcaaa cttttctatc
     1741 accaatctgt aaatccttat aaacaagatc atgactttgc tcatggatta tatcttgaat
     1801 atagtgttag aacttttggc gttgaatggg ctcatgaatg ggtaggagaa aactatgatg
     1861 cccaggtagg gtttgtaccc agaaaaggat ttctcagact taatcctgaa ctttcattac
     1921 ggttttatcc aaagtccgga ataattaata accatggtcc aagaggggaa ctctcctatg
     1981 tttggtcgga aatttatggt aagcttgatc atgaattgca aggaaattat aacatcagct
     2041 ttcaaagcgg agcaaatgct tcggttgact ttactcagaa tttcatttat ttgactgacc
     2101 ccttcgatcc gaccaacatg gagggagttg aattacccgc gttttccaaa ttcacttatt
     2161 atgattacag attgtcattc agatctaacg atcggcgggc attttatttc aatattaacc
     2221 accagggcgg agaatatttt aatggaaccc ggtatggcat aagcggcgga ttgaatttac
     2281 gtgctcaacc ttatgtgtta attactgcca acttcaatta caatcatatt aatcttccag
     2341 aacctttttc agatgccaac ttgttcctat tcggaccaaa attggatgta acctttaccc
     2401 ggaatttgtt tcttaccaca ttctttcaat tcaacaacca atcagaaaat gtgaatatta
     2461 atgcaaggct tcaatggcgg tttaagccgg tttcagatgt gtttctggtc tatacagaca
     2521 actatgcatc aaccaactgg gctgtcaaga acagagccat cgtggttaag gttacttatt
     2581 ggctaaatct ttgatccata gatagtgtga ttaagagaaa ttttggccaa ttttttttaa
     2641 atttgaacta ataaaaaccc taagtgttat ttgaagaaca atgaggaagg gaataatttc
     2701 agtttttagc acgctaatat tggttgcggt aatagtcagc gctgccttta ttactagtgt
     2761 gccggggatt aaggatctct cgttgaaagc caacaacgaa agtgttgtta agtggtatac
     2821 ttttgaagaa gcaatagaaa taaataagaa agcaccaagg aagattttcg tcgacgtata
     2881 cactgactgg tgtggctggt gcaaaaagat ggatgtcaat acatttaata accccacggt
     2941 agccgaatat ctcaatgaga aatattaccc ggtaaaattt aatgctgaac aaagggaatc
     3001 tattgttttc agagaccaca cctttaaatt tattgcacag ggtgcaaggg gttatcacga
     3061 acttgcagct gccctattga ataataaatt aagttacccc acagttgtat ttcttgatga
     3121 agactttaac atgatc
//
LOCUS       DASWHX010000191        12267 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_585587, whole genome shotgun sequence.
ACCESSION   DASWHX010000191 DASWHX010000000
VERSION     DASWHX010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 12267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12267
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_585587"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..621
                     /locus_tag="VGA21_12700"
     CDS             <1..621
                     /locus_tag="VGA21_12700"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX9651415.1"
                     /translation="VVKDFNFESLRNEIIPMVIVNLEGEFGFFSQLSIRIAGESTNEI
                     IPEVESIYKELNPQNPFIYTFLDETLECQYSSEYTSGKMLGFFTIIAIVIACVGLFGL
                     AAYSISQRTKEIGVRKVLGASVRSITLLIFSDFGRLIMAAFIIATPVSYYAMDRWLES
                     FAYKTSLNPWVFLLAGILVLFITTITISYQSISAATVNPAKSLKSE"
     gene            837..1190
                     /locus_tag="VGA21_12705"
     CDS             837..1190
                     /locus_tag="VGA21_12705"
                     /inference="COORDINATES: protein motif:HMM:NF024297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX9651416.1"
                     /translation="MESVITKSYIEPLYLYGDLEKIKEGFHEEFRMNVLYNGVYSIRT
                     RDEWIERLRNNSLAGNAVKKTYTWEFNLIDVEDQTAVVKLTVNEEGKLKYIDYLTLYK
                     FENGWRVITKQFSMY"
     gene            complement(1430..1786)
                     /locus_tag="VGA21_12710"
     CDS             complement(1430..1786)
                     /locus_tag="VGA21_12710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014021466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX9651417.1"
                     /translation="MNLKPIKTKKDYQQALDRLELIFDAKKGTKEGDELEILGILIDQ
                     YENEHFPIDLPDPIEAIKFRMEQLGYTQTDLAKVIGLKSRASEILSKKRKLSLDMIRQ
                     LHDKLNIPTDVLIQAY"
     gene            complement(1792..2085)
                     /locus_tag="VGA21_12715"
     CDS             complement(1792..2085)
                     /locus_tag="VGA21_12715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013752372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HEX9651418.1"
                     /translation="MRVIAKKILREFWAKHSDCEQQLKSWYREAEKSEWKNTNEIKKE
                     YPSASVLGNNRVVFNLKGNKYRLIVKINFHYQMMWIRFIGTHKEYDRIDANKI"
     gene            complement(2176..3510)
                     /locus_tag="VGA21_12720"
     CDS             complement(2176..3510)
                     /locus_tag="VGA21_12720"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9651419.1"
                     /translation="MRLYLHRKILIGFTTSIIILFLLAAISLLFFKGFLDTVEWGSRA
                     RELMFNLERSSALVSQIESVSTDFKIDENNNSITSLKELTISFSENMRAIISLTKDEI
                     NKEKISELQILANSLIHQIKMSTEPDSAGASVIGELARSIIILVNRIQAEENLLIKDR
                     SIKAGVWFRQFAFTFLLLVTFILLLMTFLIIKVNRTLKLRFETEGKIKAAELSTRKAN
                     AELESFSSSVSHDLRAPLRTIAGYAEILKKSQGPHLTPDGNRLIAIIISNSLTMEKLI
                     DDLLNLSKVNRDTLTRTTVDMELFVKPIWNEVLKQAGNPEVEIVWKELGRANIDINLF
                     RQVWINIFSNAIKYSGKKEKAQVEVGTITRNAGRTYYVKDNGAGFDMKYSDKLFGAFQ
                     RLHKQSEFEGTGVGLALVKRIVERHKGKVWAESIVKEGATFYFTIPDSLKPE"
     gene            3894..4790
                     /locus_tag="VGA21_12725"
     CDS             3894..4790
                     /locus_tag="VGA21_12725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008198470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9651420.1"
                     /translation="MKLVIENLSKTYPGNIQALVNINIEVDQGLFGILGPNGAGKSTL
                     MRTIATLQEPDIGSIHLDDINVLTEKTKVRKILGYLPQEFGVYPNLTANNMLNHIAVL
                     KGVTIRGERKELVDALLHKVNLWTYKNKNMGTFSNGMKQRFGIAQALIGDPTLIIVDE
                     PTAGLDPAERNRFLNLLSELGENVIVILSTHIVDDVKELCSRMAIISEGRLLYIGSPG
                     HAISELNGKVWSRFVEKSELPEFRLSMNILFEKLIAGKQGIHVLSDEKPGPEFWQVQA
                     GLEDVYFAHLLRAGEQTSQLMN"
     gene            4795..8418
                     /locus_tag="VGA21_12730"
     CDS             4795..8418
                     /locus_tag="VGA21_12730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEX9651421.1"
                     /translation="MFTEFFFFELKYRFARKITYIYFVLIFVLSFLIVTSPTLKIAGA
                     VGQTAANSPYTIALLQSILSFVFTIITSAFISVAIIRDFEHNTSGIIFTTPLSKFGYL
                     FGRFSGSVVIVILVQTAISFGFMAGFAVGEYLPWDVAWINRKLLPFNLLHFVQPFLLF
                     TVTNIFITGALFFSSGSLGRRPLFIYMQGVLLVIVYQIGYNLMGNLRMEKWVAILDPF
                     GVNTFQFVTRYWTPVEQNAMLISLDGTFLVNRIIWIALGLLSLLFTYFRFSFHKKSLA
                     FSTPGKEKKMSVLPNAISLIIPKVRKTINLKTYWVQFTRLTLDHFRMIWKERPFLIIM
                     ASGALVIIANALKITSVYGTPIYPTTSEILSLLGSFNLFFIIIMIFYSGELIWKERSS
                     KVNLIVDSLPVPTGINLFAKICSITLIYISLFILLTLSGITIQIIQGYYEFELSAYFY
                     TLYVDRLTSLFLFTVLVFFIQIVINNRYMGFAVCILVVVFRSYMGRLGIESGLLQYAG
                     GSLGIFSEMNLYGHFVEPFTWLRTYWSAFAGSLTAICVLFYLRGYDNSLGSRLKVAVS
                     RVKGPIMVFSLISLIVFILSGFYIRYNVSRLNRFDHSDNLKSWKAEYEVKLKKFQYLN
                     QPKIINTVLNVSINPASRDFIIDGNYTLINNSSHVINELHISKSSDPNILIEKVSFDK
                     DFSLVKKHDNLGFAIYQLAQPLLPGASMNMGFRVSFITKGFVNGATNLDIVYNGTFIS
                     NDYLPGLGYNSAVELTEKDDREDFNLPKLQLTSSGNNKQIGIFGNDADMINLDITVST
                     SSDQLVVGPGKLKAKWSEGSKLFYHYVTTRPISNYYAIVSGNYKVIEDNWKGVNLEIY
                     YHPEHLNNVGGMMMALKESMNYYTSNFGSYQNDNLRIVELPRYKSHAQSLATTITFSE
                     AIEFVSRVKDRNIDLDIVSFVTANETASQWWKHNISIADTQGMKLISEGIGRYSSLMV
                     LQNSYPGQTLEKYLEYELDAYLRGRSMDRKKEQPLYLVENDQAYVYSHKAALIFHALQ
                     DYLGEKKLNDALREFRENWQSGSVRYATSIDLINEIRKIVPDSLNNLIDDYFFNITLF
                     NNSISDGAYKKNPDGSFEVTMNLQAHKLVLDSLGNEIEVPINDWIDVGVYGKDISGQD
                     SLIYLRKYKFSNSAEILSISVNRLPTIAGIDPLKKLIDQHPNNNKVKLKEFIDVSNLF
                     IGF"
     gene            complement(8580..9773)
                     /locus_tag="VGA21_12735"
     CDS             complement(8580..9773)
                     /locus_tag="VGA21_12735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019948198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate/molybdate transporter"
                     /protein_id="HEX9651422.1"
                     /translation="MNTQTKWTLRDIKFNKQELAGSFGDIGTDLPLIIAMILAAGLHT
                     PSVLIVFGLMQIFTGLIYKMPMPVQPLKAMATLVIVQQISSNILFGAGLAIGIVMLAL
                     SVSGLLTKLSQIIPKAVIRGIQLGLGLKLCMLAFQKYIPDQGIPGYILAFVVFVLIIF
                     LLDNKKYPASLFAIALGVIFAFLFNIDSNTLQYAFGFNLPLLFTPTIDDVTTGFLLLA
                     LPQIPLSLGNSIIATKQVSHDLFPDRKPLTIKKIGLTYSLMNLVIPFFSGIPSCHGSG
                     GMVGHYTFGGRTGGSVILYGLFYLILGLFFGNGFENIVQIFPLPVLGVILVFEGLALM
                     ILVKDLMKNQRVFVIALLVGVIASGLPYGFVISLVVGTILYYTPSKLSTLKNIGNNQT
                     DKNEI"
     gene            complement(9831..10538)
                     /locus_tag="VGA21_12740"
     CDS             complement(9831..10538)
                     /locus_tag="VGA21_12740"
                     /inference="COORDINATES: protein motif:HMM:TIGR00035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HEX9651423.1"
                     /translation="MKTLGIIGGLSWFSTTVYYKTINQLTNERLGGSHSARLLLYSVD
                     FNDFKVLQEKNDWGKIEHMLTDIALRLQSAGADCIVMATNTPHLVADIVRQKIKIPLL
                     HIAEETAKEIIRQKINKVGLLGTKFTMENAFFKDRLTKFGINTVIPDSSDRDFIHATI
                     FNEFTKGIFKDETKKKYIDIIAKLKRDGAEGVIFGCTEIGLLITQDDSNITVFDTTAI
                     HSKAAVDFALSTELVTE"
     gene            complement(10704..12158)
                     /locus_tag="VGA21_12745"
     CDS             complement(10704..12158)
                     /locus_tag="VGA21_12745"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX9651424.1"
                     /translation="MKESITIIFLLVAILAYAQIDENTELFKTLKVQDSTFFERGFNR
                     CDLDFLGNHIAGNLKFYHDQSGFQDRKAFFENTEKYICSNSDKKPIRKVDVNSLEVFP
                     LYNDGNLYGAIQKGIHHFYLRESGKEDIWTSTAKFTHIWVLESKTWKLSEVLSYDHHD
                     PVFDEPRNNNLEKLLKENNVPALGIGVIENGKLTKVEVYGTLDKQATAPYNTIFKVAS
                     LTKPIVALTTLKLIDKGILDMDEPLYKFWIDPDIKEDKRYKKLTPRLVLSHQTGFPNW
                     RYMTETNKLAFQFDPGTKYQYSGEGFEYLRKAIETKLGKSIEELTNELLFSPLQMTDT
                     RFWWDNKMDESRYAQNFDADGNILPTIKYYEANAAANLLTTVENYGKFLAYVINGAEL
                     SENLFQEMQNHQVELKVNNYFGLGWEILTDFSNNEFALLHTGKDPGVSTLAIIFPKSK
                     NGYLIFLNGDNADKIYEYLLTKMLYLGVELWNKR"
BASE COUNT         3666 a         2275 c         2305 g         4021 t
ORIGIN      
        1 gtggttaaag atttcaattt cgaatcattg agaaatgaga ttatcccgat ggttattgta
       61 aaccttgaag gagagttcgg attttttagc cagctttcca taaggatagc cggtgaaagt
      121 acaaacgaaa tcattccgga agttgaaagc atttataaag aattgaaccc gcaaaatcca
      181 ttcatttaca cattcctgga tgaaacgctg gagtgccaat attcttcaga atatacttcg
      241 ggtaagatgc tgggcttctt tacaataatc gcgatagtga ttgcctgcgt aggactattc
      301 gggttagctg cctactccat cagtcagcgc accaaggaga ttggtgtccg aaaagttttg
      361 ggcgcaagcg tgaggagcat aacgctttta atattcagcg attttgggcg gttgatcatg
      421 gccgctttca ttatcgcgac tcctgtgtcc tattacgcca tggaccgctg gcttgaaagt
      481 ttcgcataca aaacttctct taatccctgg gtgtttttac ttgcaggaat attggtgttg
      541 tttattacga caattaccat ttcataccag tctattagcg cggccacagt gaatccggca
      601 aaaagtctca agtcagaata gaaagttgat tttggacgga agacagatgt attttgaaag
      661 aaccggtggc tattgttcaa tgaaaagaat aaagttatgt tgttttaagt ataacaagta
      721 attgcaattc atgtttgatt taaattaaac ctgtcatgaa acatctaact attttactgg
      781 tattgttgag tgcaaccacc atttttgcgc aagacaatta gtcggcaaaa aaggacattg
      841 aatccgttat taccaaaagt tacattgaac ccttatacct gtatggcgat ttggagaaga
      901 ttaaagaagg gtttcatgaa gagttcagaa tgaacgtctt atataatggt gtttacagca
      961 ttcgaacccg cgatgagtgg attgagcgac tcaggaataa ttccctggct ggaaacgcgg
     1021 taaaaaaaac atacacctgg gagtttaatt tgattgatgt agaagaccaa accgccgtgg
     1081 taaaacttac tgtaaatgaa gaaggcaaac ttaaatatat tgattacctg acgctttata
     1141 aattcgaaaa tgggtggcgc gtaattacaa aacagttttc gatgtattga aatcctgcaa
     1201 ttaatccgcg cctgaccttc caattttctt catgatttca tcctgaattc atttcaggat
     1261 catagcaatt agcaattgct tttttgctga ggtggttatt gtttacaagc taggccagtt
     1321 ttagcattct gctaacgttt tgggtttgtt tagtggcgga taaatccgga ggatctgtac
     1381 gcctatacgg aaataattag acaaatccgg cgttggcggt ttgtgccgtt tagtaggcct
     1441 gaattaaaac atctgtcgga atgttcaact tgtcgtggag ttgtctaatc atatccaacg
     1501 atagctttct cttcttactc agtatttcac ttgctctact tttgagtcca atgacttttg
     1561 ctaaatctgt ctgagtataa ccaagttgct ccattctgaa ttttatcgct tcaattgggt
     1621 caggcaagtc aattgggaaa tgttcgtttt cgtattggtc aatcaatatt cccaatatct
     1681 caagttcgtc accttcctta gttcctttct tagcgtcaaa aattaactca agtctgtcca
     1741 acgcttgttg gtagtccttt ttagtcttaa ttggttttaa gttcatagtc atcaaatttt
     1801 attcgcgtca atcctgtcgt attctttatg agtcccaata aatcgtatcc acatcatttg
     1861 ataatgaaaa ttaattttaa caattagtcg gtatttgttt cctttcaggt taaaaacaac
     1921 tctattattc ccgagaacac ttgctgatgg gtactctttc tttatttcat tggtattttt
     1981 ccactcactc ttttcagctt ctcgatacca ggatttaagc tgttgttcgc agtccgaatg
     2041 tttagcccaa aactctcgca gtattttctt agcaataact ctcaagtctt aatgttttgt
     2101 caaaggtagg gaagagttcc cgtattggga aattttattt gataaaaaat ctgctttttt
     2161 ttggtcgtgc aattgtcatt ccggtttcaa agaatcaggt attgtgaaat aaaaagttgc
     2221 accctccttc acgattgact ccgcccaaac tttaccctta tgacgttcta ctatcctctt
     2281 aaccagggca agcccgacac cagtaccttc aaattctgat tgtttgtgaa gtctttgaaa
     2341 tgccccaaat aatttgtctg aatatttcat atcaaagcca gctccattgt cttttacata
     2401 ataagttctg ccggcgttcc gggtaattgt cccgacttca acttgggctt tttctttttt
     2461 gcccgaatat ttaatagcat tgctgaatat gttaatccac acttgccgaa agagatttat
     2521 gtcaatattt gcccgtccca attctttcca aacgatctca acttcaggat tccctgcttg
     2581 ttttagaact tcattccaga ttggtttaac gaataattcc atatctactg ttgttctggt
     2641 aagtgtgtcc cggttaactt ttgacaaatt aagcaggtca tctattaact tttccatcgt
     2701 taatgaattg ctaataataa tagcaatcag cctgttgcca tcaggggtta aatgaggacc
     2761 ctgtgatttt ttcaaaatct ccgcgtaacc tgcaatcgtg cgcaaaggcg ctctcaaatc
     2821 atgagatacc gacgagctga atgattccaa ttcagcattt gcctttcttg tagataactc
     2881 agccgctttt attttccctt ctgtttcaaa tcgcaactta agtgtcctgt ttaccttaat
     2941 tatgaggaag gtcattaaga gtaaaataaa agttacgagc aggaggaatg taaaagcaaa
     3001 ttgtcggaac cagacgccag ctttgatgga cctgtccttg attaaaagat tttcttcagc
     3061 ctggattcta ttcaccaata taataattga ccttgcaagt tcaccaatta cacttgcccc
     3121 ggcagaatcc ggttcagtag acattttaat ttgatgaatt aaagagtttg caagaatttg
     3181 taattcgcta attttttctt tatttatttc gtccttggtt aaagaaatga tagctctcat
     3241 attttcactg aacgaaattg ttaattcttt aagagaggtt atggaattat tattttcatc
     3301 aattttgaag tcagttgaga cactttcaat ttgactaacc aatgcactgc ttctttccag
     3361 gttaaacatt aattcccggg cacgggagcc ccactcaacg gtatccagaa atcctttaaa
     3421 aaataagaga gatatcgcag cgagaagaaa aagaataata atagaggtgg tgaaacctat
     3481 aagaattttt cgatggagat agagtctcat aaagtattaa tacatttaat gtagttaata
     3541 aaaaatcaaa acattttcat aaagtattaa tacaatatgc caatttaata ttcccacagg
     3601 atacaataaa ttgaacgtat agtaaattta ccaaagtaat aatcaaataa gactattaat
     3661 gttagccatg taagttagaa ttcataatta cagttttttg tagttctccg taatttttat
     3721 catatttttt ctttgcaggt ttcagttacg ggtgattaaa taatcgacat gcttaatccg
     3781 ttggcatcaa tgaaggagta aacagcatac ttcttacaga atggtattga tgccaaattg
     3841 atgttataca aaatttgaac tatccttatt tagtcataat tgaatagatc aaagtgaaat
     3901 tggtaataga aaatctgagt aagacatatc ctggaaacat ccaggcactg gtaaacatta
     3961 acatagaagt tgatcaggga ctctttggta tacttggccc taatggagcc ggaaaatcta
     4021 cgttaatgcg tactattgct acgctccagg agcctgatat tggatcaatt catttagatg
     4081 acattaatgt gctaactgag aaaacaaaag tgagaaaaat actcggttac ttacctcagg
     4141 aatttggtgt ttacccaaat ctaacagcaa ataacatgct taaccatatt gcggtgctta
     4201 aaggagtcac gatacgcgga gaaaggaaag aacttgtaga tgctcttctg cacaaggtta
     4261 atctttggac ttacaaaaac aaaaatatgg ggacattctc gaacgggatg aaacagcgtt
     4321 ttggaattgc tcaggcgttg atcggcgatc caacattaat aattgttgat gaaccgaccg
     4381 ctggacttga tcccgcggaa agaaaccgat ttttgaatct cctttccgag ctgggtgaaa
     4441 atgtaatagt aattctttcc acccacatag ttgatgatgt aaaagaactc tgctcacgga
     4501 tggcgattat aagcgaaggc aggttgcttt acattggaag tcctggtcat gccattagcg
     4561 aattgaacgg taaagtgtgg agcagatttg tcgaaaagtc tgaattgcca gagtttagat
     4621 taagtatgaa tatacttttt gaaaagttaa ttgcaggaaa gcagggaatt catgttttga
     4681 gtgatgaaaa accgggtcct gaattttggc aggtacaggc agggcttgaa gatgtttatt
     4741 ttgcccattt acttagagct ggggagcaaa catcacaact tatgaattga taaaatgttc
     4801 acagaattct tcttcttcga actaaagtat cgtttcgcga ggaaaataac ttatatatat
     4861 tttgtcttga tttttgtgct cagcttttta attgtaacca gccctacact taaaattgcc
     4921 ggagctgttg gccaaaccgc cgccaactca ccatatacga ttgccttact tcaatccatt
     4981 ttatcttttg tatttacaat cattacttct gcattcatta gtgttgcaat tatccgggat
     5041 tttgagcaca acaccagcgg gatcattttt actactcccc tttcaaaatt tggttattta
     5101 tttggtcgct tctcagggtc cgtagttata gtaattctgg tacaaactgc aattagtttc
     5161 ggatttatgg caggatttgc cgttggagaa tacctgccct gggatgttgc atggattaac
     5221 aggaaattac ttccctttaa tttgctccac tttgttcagc catttttact ttttacagta
     5281 acgaatattt ttattacggg tgcattattt ttttcaagcg ggtcgcttgg cagaagacca
     5341 ttgtttattt atatgcaggg tgttcttctg gttatcgtat atcagattgg atataatttg
     5401 atgggtaacc tcaggatgga aaaatgggtt gcaattctgg atcccttcgg ggtaaataca
     5461 tttcaatttg taacaaggta ttggacgccg gtcgaacaaa acgccatgct tatttccctc
     5521 gatggcactt tcctggttaa ccgcataatc tggattgctt tgggcttatt atcgctctta
     5581 tttacctatt tccggttctc ttttcataag aaaagcctgg cgttctcaac accagggaaa
     5641 gaaaaaaaaa tgagtgttct tccaaatgca atttcactaa ttatcccaaa ggtcaggaag
     5701 actataaatt tgaaaacata ttgggttcaa tttacaaggt taacacttga tcactttaga
     5761 atgatttgga aggaacgacc cttcctgatc atcatggcca gcggtgccct ggttataata
     5821 gcaaatgctt tgaaaatcac ctcagtctat ggtacaccca tttatcctac taccagcgaa
     5881 attctttcat tgttgggttc attcaatcta tttttcatta tcataatgat tttttactcg
     5941 ggagaattga tctggaaaga gcgcagttcc aaagtaaatt taatcgtcga ctcactaccg
     6001 gttcctaccg gaatcaattt gtttgccaag atctgttcca taacattgat ctatatttcc
     6061 ttgtttattt tacttaccct ttcgggaata actatacaaa taattcaggg atattatgaa
     6121 ttcgagttat ccgcatactt ctatactctt tatgtagaca gattaacctc ccttttcctt
     6181 tttaccgtgc ttgttttttt tattcaaatt gtcattaata acagatacat gggatttgcg
     6241 gtttgcatcc tcgttgtcgt gttcagatcg tatatgggaa gattgggtat cgaaagcggc
     6301 cttttgcaat atgccggcgg aagtcttgga attttttccg aaatgaactt atacggacat
     6361 tttgttgagc catttacatg gctcagaact tactggtcgg cttttgccgg atcgctgact
     6421 gccatttgcg ttttatttta cttgcgcgga tatgacaatt cacttggcag caggttaaaa
     6481 gtggctgtgt cccgagttaa aggtccgatc atggtattct ctctaatttc acttatcgta
     6541 tttatattat ctggatttta catcagatat aacgtctcaa gattaaaccg ttttgatcat
     6601 tcggataatt tgaagtcctg gaaggctgag tacgaagtga aattgaaaaa atttcagtac
     6661 ctcaatcaac caaagataat taataccgtc ttaaatgtga gcataaatcc agcatcacgc
     6721 gatttcatta ttgatggaaa ttatacctta attaacaatt catcacatgt aataaatgag
     6781 ctgcatatta gcaaaagctc tgatcctaat attttaattg agaaggtcag ttttgacaaa
     6841 gatttttctt tagtgaagaa acatgataat ctgggctttg caatatacca actcgcacag
     6901 ccgctgttac ctggtgcaag tatgaatatg ggtttcagag tttcttttat caccaaaggg
     6961 tttgtaaacg gtgcaactaa ccttgatata gtctataatg gtacttttat ttccaatgat
     7021 tatctccccg ggttaggata taacagcgca gttgaattaa ctgaaaagga cgatcgggaa
     7081 gattttaact tgccgaaact gcaattaaca tcttctggca ataacaaaca gattggaatt
     7141 tttggcaatg atgcggatat gataaacctc gacatcactg tgagtacttc gtctgatcaa
     7201 cttgttgttg gcccggggaa actaaaagct aaatggagcg aaggaagcaa actcttttac
     7261 cactatgtta caacgcggcc catttccaac tattatgcaa tagtatcagg caactacaag
     7321 gtaattgagg ataattggaa aggagtaaat cttgaaatct attatcaccc tgaacactta
     7381 aataacgttg gtgggatgat gatggccctt aaagagtcga tgaattatta tacaagtaat
     7441 tttgggtctt atcaaaatga taatttgagg attgttgagc ttccaaggta taaatcccat
     7501 gcgcaatcat tagctaccac aattaccttc tctgaagcaa ttgaatttgt ctcaagagtt
     7561 aaagatcgga acattgattt ggatatagtt tcgtttgtta ccgcgaatga aactgcaagt
     7621 caatggtgga aacataatat ctcaattgca gacactcagg gaatgaaact gatctctgag
     7681 ggaataggaa ggtattcatc actcatggtg ctgcaaaatt cttatcccgg acaaacgctg
     7741 gaaaaatatc ttgagtatga acttgatgcc tatctccggg gcagaagcat ggaccgaaag
     7801 aaagaacaac ctctatattt ggttgagaac gatcaagctt atgtttatag ccacaaggcg
     7861 gccttgatat tccatgcgct tcaagactac ctgggtgaga aaaaattaaa tgatgcttta
     7921 agggaattta gagagaactg gcaatccggc agcgttaggt atgcgacctc aatagatttg
     7981 attaatgaaa tccggaaaat tgttccagat agtcttaaca atttaattga tgattatttt
     8041 tttaacatta ccctttttaa taatagtatt tcagatggag cgtacaagaa aaatcctgat
     8101 ggatcttttg aagttacaat gaatctacag gcacacaagt tagtattaga cagtttaggt
     8161 aatgaaattg aagtcccgat taatgactgg attgatgtgg gagtttatgg gaaagacatt
     8221 agcgggcaag acagtcttat ttaccttcgt aaatacaagt tttcaaattc agcagagatt
     8281 ttgtccattt ctgtgaacag gctgcccacc atagcaggga ttgatccatt gaaaaagctg
     8341 attgaccaac acccaaataa taataaggtt aagctgaaag aatttattga tgtcagcaat
     8401 ctctttattg gattttgaaa cccaaacctt ttgtaacctg tcagtagttt gtaatacgga
     8461 cttccggttt ctgccgctgt ttattaattg ctgcacgaca gcaataccgg aaaccagggt
     8521 aattaccgca tgtcctgact gggcggcaaa gaaccgggat gaatactcag accggagatt
     8581 taaatctcat ttttatcggt ttgattattt cctatattct tcaaggtgct caatttggaa
     8641 ggagtataat ataaaatagt tccaacaact aaagatatga caaacccgta agggagacca
     8701 gacgcaataa ctccaaccaa taaagcaatg acaaaaactc tttggttttt cattaaatct
     8761 ttgaccaaaa tcatcaaagc caatccttcg aatacaagta ttacccctaa taccggaagt
     8821 ggaaatattt gaacaatatt ttcaaatcca tttccgaaaa ataatcccag gatgagataa
     8881 aaaagtccgt atagaattac cgacccgccg gttctgcctc caaaggtgta atgcccaacc
     8941 ataccgcccg aaccatgaca gctgggtatt ccactaaaaa atggtatcac gaggttcata
     9001 agggaataag tcaatcctat tttttttatc gttaaaggct ttctgtcggg aaataaatca
     9061 tggctaacct gttttgtcgc tataatagaa ttgcccaatg acagcggtat ctgaggtaat
     9121 gctaataata aaaacccggt cgtcacatcg tcaattgttg gagtgaacaa caggggcagg
     9181 ttgaaaccaa aggcatactg cagcgtgttt gaatcaatat taaataaaaa agcgaagatt
     9241 acaccaagcg caatcgcaaa caaagacgcc ggatactttt tgttatctaa aagaaaaatg
     9301 atcaatacaa aaaccacaaa tgccaaaata taacctggta tcccctggtc aggaatatat
     9361 ttttgaaaag caagcatgca tagttttaac cctagtccaa gttggattcc tctaattact
     9421 gctttgggga ttatttgact gagtttggtc aaaagtccgg aaacggaaag ggccaacata
     9481 acgataccta tggcgagacc ggctccaaat aatatgttgc tacttatttg ttgtacaatt
     9541 actaaagtgg ccattgcttt tagtggttgt acaggcatgg gcattttata aatcaatccg
     9601 gtaaatatct gcattaagcc aaatacaatg agtacactgg gtgtatgcaa acctgcggca
     9661 agtatcatgg caatgatgag gggaagatcg gtgccaatat ccccaaaaga tcccgccagt
     9721 tcttgcttat tgaattttat atcccttaat gtccatttcg tttgagtatt catggagatt
     9781 tattcattaa ccgctaacag attgcggctt ggtgattatt tcatctctct ttattcggtt
     9841 actaattctg ttgatagcgc aaagtcaact gctgctttag aatgaattgc tgtcgtgtcg
     9901 aaaactgtta tgttactgtc atcctgtgtt attagcaaac caatttctgt gcaaccgaaa
     9961 attactcctt ctgctccgtc tctttttaat ttggcaatta tatcaatgta ttttttcttt
    10021 gtctcgtctt tgaaaattcc ttttgtaaac tcgttgaaaa tggtggcgtg aataaaatct
    10081 ctgtcggaac tgtccggaat aacggtgtta atcccaaatt ttgtcaagcg gtctttaaag
    10141 aaagcattct ccattgtaaa ttttgtccca agcagtccga ctttgtttat cttttgtctg
    10201 atgatttcct ttgctgtctc ttctgcaata tgaagcaatg gaatttttat tttttgtctt
    10261 acgatatccg cgactaaatg cggtgtattc gttgccatta caatacaatc tgcaccagcg
    10321 ctttgaagtc gcagtgcaat gtcggtgagc atatgctcaa ttttgcccca gtcatttttt
    10381 tcttgcaata ctttgaaatc attaaagtca accgagtaaa gtaaaagtct ggcagagtgc
    10441 gaaccaccaa gtcgctcatt tgtcagttga ttaattgtct tgtagtaaac tgtggttgaa
    10501 aaccaactta gtccaccgat aattcctaat gttttcattt acttttgttt cattttcagg
    10561 gttacttata acggcaggta ttaaaccagt agcggaataa aaaccgttaa acttatcgga
    10621 tagcaagata cttaattaaa tacaaaacgg ttcaggttaa taccaaaacc gctatcgcag
    10681 ttatacattc taatgcaacg cttttatctt ttattccaca attcaactcc cagataaagc
    10741 atctttgtaa gcaggtattc atagatttta tctgcattgt cgccatttag aaaaattaaa
    10801 tacccattct ttgattttgg aaatattatt gccaatgtgc ttacgccggg gtctttcccg
    10861 gtatgcaata aagcgaattc attattgcta aaatctgtta agatctccca acctaatcca
    10921 aaatagttat taacttttaa ttcaacctga tgattttgca tttcctggaa taggttttca
    10981 gaaagttctg cgccattgat tacgtaagct aaaaatttcc cataattttc aacagtagtc
    11041 aaaaggtttg ccgcagcatt tgcttcataa tattttattg ttggtagtat atttccgtca
    11101 gcatcaaagt tttgagcgta tcttgattca tccattttat tatcccacca aaagcgagtg
    11161 tcggtcattt gcaaaggtga aaaaagcaat tcattagtta gttcttctat acttttgcca
    11221 agtttggttt ctatggcttt tcgcaaatat tcaaatcctt ctccggaata ttgatattta
    11281 gttccgggat caaattgaaa agcaagttta ttggtttcgg tcatataacg ccaatttgga
    11341 aatccggttt ggtggctaag cactagtctt ggtgttaatt ttttgtatcg tttgtcttct
    11401 ttaatatccg ggtcaatcca aaacttgtac aatggttcat ccatatccag tatacccttg
    11461 tcaataagtt ttagggtagt tagcgcaaca ataggtttgg tcaatgacgc taccttgaaa
    11521 atggtgttgt aaggtgcagt agcttgcttg tctaatgttc cataaacttc aactttggtc
    11581 aatttgccat tttcaattac accaattccc agggcgggta cattgttttc ttttaataat
    11641 ttttctaagt tattattcct gggttcgtca aacacagggt cgtgatggtc gtaactcaac
    11701 acttcgctta acttccaagt cttgctttct aaaacccaaa tatgggtaaa tttggctgtg
    11761 cttgtccaaa tatcttcttt cccgctctct ctcaaataaa aatgatgaat tcctttttga
    11821 atggcaccgt aaagatttcc atcgttgtaa agaggaaaaa cttccaagct gtttacatct
    11881 acttttctaa taggtttttt atcagaattt gaacaaatgt atttttctgt attttcaaaa
    11941 aatgcctttc tgtcttgaaa cccactttga tcgtgataaa attttaaatt gccggctata
    12001 tggtttccca gaaaatccag gtcgcaccgg ttaaacccac gttcaaagaa agtgctatct
    12061 tgaactttca gtgttttaaa caattcagtg ttttcatcta tttgagcgta agctaaaata
    12121 gcgactaata agaaaattat cgtgattgat tctttcattg agtcttcgtt tcttttgatt
    12181 ttaaatgttg ccgtatggtt cgggacttaa cgaagtgacg gcaaatagat gcaccatact
    12241 ttcgccctgc accaaagtta attaaat
//
LOCUS       DASWHX010000192         1977 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_202528, whole genome shotgun sequence.
ACCESSION   DASWHX010000192 DASWHX010000000
VERSION     DASWHX010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 1977)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1977)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1977
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_202528"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            370..627
                     /locus_tag="VGA21_12750"
     CDS             370..627
                     /locus_tag="VGA21_12750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015030231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HEX9651425.1"
                     /translation="MIAANYTEFRTRLKKYLDNVEENNETLIIKRGTGKGTVLISLEE
                     YNAIMETMHLLSSKANADRLYESIKQMKSRKVVRKGLIEGK"
     gene            690..773
                     /locus_tag="VGA21_12755"
     CDS             690..773
                     /locus_tag="VGA21_12755"
                     /inference="COORDINATES: protein motif:HMM:NF018476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YoeB family
                     toxin"
                     /protein_id="HEX9651426.1"
                     /translation="MLKKINELIKDIQRNPFEGKGNQNLKI"
     gene            739..882
                     /locus_tag="VGA21_12760"
     CDS             739..882
                     /locus_tag="VGA21_12760"
                     /inference="COORDINATES: protein motif:HMM:NF018476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YoeB family
                     toxin"
                     /protein_id="HEX9651427.1"
                     /translation="MKVKETRTLKYDLSGLWSRWIDREHRLFYQTKAKEILIYSCRYH
                     FGR"
     gene            complement(982..>1977)
                     /locus_tag="VGA21_12765"
     CDS             complement(982..>1977)
                     /locus_tag="VGA21_12765"
                     /inference="COORDINATES: protein motif:HMM:NF025587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase family protein"
                     /protein_id="HEX9651428.1"
                     /translation="YNLVEKEGQDQFDEEYEIKTCDMKLFFEGGDEGKKQFARDLGAA
                     MQGIGFAILEGHGVDSTLYAKAEEKVAEFFETTTREQRIRYLAHRQGSVNQGYFPMKE
                     TTIIHPDLVEGWVFCRRAFNLGENKDYKEEDFWPRPGYEPFFRNIILEHERLILPLMQ
                     SMLSYLGCDPNLYNEKFTKTNFGFRLNYYPPLQQEDLNSGAGRMLGHEDVDFFTILPA
                     QSVDGLQVLNRQNMKWIRLKAPDGSIVLNTGDYMQRITNDIFPSTTHRVSKPRDHSLI
                     SKPRISIPMAVYVWEHETLEVLPCCGEPKYKPINAETFHTKITSKYYGDDYAEQK"
BASE COUNT          592 a          391 c          406 g          588 t
ORIGIN      
        1 ctttgttagc aatgcggtaa tcaatggcat atatgtatta cgaatgtgta ttgtcaactt
       61 cagaacttcc aaattcgatg tggaagcttt accggaaaga gttattgaat tgggaaggga
      121 attgcattaa caaaaaagca aatgtcgcat cgtgatttct aataaaccgc caggtttctt
      181 taattaataa ttctgataga tcttcccgct ttaggtgcac actgcatcag gggtgagaga
      241 aaatttctgg tttgaatctg tgatgacaat gttaagcgat acggtgcctg aatggccatt
      301 ttttaattca aatacataac attttcgaat tacacaataa gtacgtatat ttgtacttaa
      361 ttaattctta tgattgccgc taattatacg gagtttagaa ccaggttgaa aaagtatttg
      421 gataatgtgg aggagaataa tgaaacactc ataattaaac ggggtacggg caaaggcacg
      481 gtcctgattt cgctggaaga atacaatgcc attatggaga cgatgcattt gctgagttca
      541 aaggcaaatg ccgacaggtt gtacgaatct attaaacaga tgaaatcaag gaaagtggtt
      601 cgcaaaggat taatagaagg aaagtgagaa tcgtattctc caaaaactca tgggaggatt
      661 acacttcctg gttaaaagag gataagaata tgctgaaaaa aataaatgag ttaattaaag
      721 atattcaaag aaatcctttt gaaggtaaag gaaaccagaa ccttaaaata tgacctgtcc
      781 ggattatggt cgcgctggat tgatcgggag cacagactgt tttaccagac aaaggcgaaa
      841 gagattttga tttatagttg tcgatatcat tttggcaggt aaccattatg cctgacccgc
      901 accatacctc ggatctaaac cttcctgccg taaatcagta agtttgaagt gtcttcaggt
      961 aatggatatt ttttcttaaa tttacttttg ctcagcgtag tcatccccgt aatatttgct
     1021 ggtaattttg gtgtgaaaag tctccgcatt gatgggcttg tatttaggtt cgccgcaaca
     1081 aggcaacact tccagcgttt catgctccca aacgtatacc gccatgggaa ttgaaatcct
     1141 gggcttgctg ataagagaat gatctcgcgg cttgctcaca cgatgtgttg tgcttgggaa
     1201 aatatcattg gtgatgcgct gcatataatc acctgtattt agaacaattg agccatcggg
     1261 agctttgagc cgaatccatt tcatgttttg ccggttaagg acttgcaaac cgtccacact
     1321 ttgagccggg aggatggtaa aaaaatcaac atcttcatgt cccagcattc ggcccgcacc
     1381 agaattcaaa tcttcttgtt gcagtggcgg gtaatagtta agtctgaatc cgaaattggt
     1441 tttggtaaac ttttcattat aaagattagg atcacacccc agatatgaaa gcatgctttg
     1501 cattaaagga agtattaacc tttcatgttc cagtataata tttctgaaaa aaggttcata
     1561 acccgggcgg ggccagaaat cttcttcctt atagtcttta ttttcaccca ggttgaatgc
     1621 tctccggcaa aaaacccaac cctcaacaag gtcaggatgg ataatagtcg tttctttcat
     1681 agggaagtat ccctgattta ctgagccttg tctgtgagca agatatctaa ttctttgctc
     1741 ccgggtggtg gtttcaaaaa actcagccac cttttcttct gcctttgcat aaagggtcga
     1801 gtcaacacca tgaccttcaa gaatggcaaa tccaattccc tgcatcgcag ctcccaaatc
     1861 cctggcaaat tgtttctttc cctcgtcacc cccctcgaaa aacagcttca tatcgcacgt
     1921 ctttatttca tattcctcat caaactgatc ctggccctct ttctccacca aattata
//
LOCUS       DASWHX010000193         6912 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_2801543, whole genome shotgun sequence.
ACCESSION   DASWHX010000193 DASWHX010000000
VERSION     DASWHX010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 6912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6912
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2801543"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1923)
                     /locus_tag="VGA21_12770"
     CDS             complement(<1..1923)
                     /locus_tag="VGA21_12770"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9651429.1"
                     /translation="MKSTKAKPPKWATRFIEWYCKPDIAEDLQGDLNEYFDENLRTRG
                     PFYARFNYVIDAFKFFRPYTLKKFQFINLITNWIMLGSYIKTSGRNIIRNKLFSAINI
                     VGLAISMSVGLLLISIISDLKSYDLFHMNKDRIYRVNSTWADQKDDPWPYASTSIKTG
                     LLLRENMAGAEAVSILRRNFSGDAHFGEKIIPVRGLWADNSFLSIFSFPMLEGNPGNA
                     LAEPYSIVLTEQTSKKIFGIESGLGKIVTLDTTNYVVTGILKDIPEFSHIKFEALGSF
                     STFDIQNRNNDRLYGWTSMWMNYVYVLLPDKGKTTGLQANLNNLSDTENSVLEHIKIS
                     LWLQPMSQFVLGPNLSNNDGANMDPVILWIVGVLAFVVMLSAAFNYTNLSIARSIRRG
                     REVGVRKVIGANRGNVIWQFMSESIVISLLALLLSAGVFILLRNQFLTIDPELLEIVS
                     LKISPAVIIGFILLAIFVGFISGILPAIFFSRISPVKVLKNAASIKGFRHLNFRKALI
                     AIQYTISLMFITATVIGFNQYKHSLAFNLGFTTDNILNVRLQGNDAAIMRKEFSEIPE
                     IIRISGSNLVPSIGNFWSASLKYQDPNDSIFVYFVQVDENYIPLHDHKLIAGRNYSGK
                     SGKFEESEIIINEKTLE"
     gene            complement(1920..2252)
                     /locus_tag="VGA21_12775"
     CDS             complement(1920..2252)
                     /locus_tag="VGA21_12775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012925542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX9651430.1"
                     /translation="MKGTNLGEFEEVVLLTIASLSDDAYSVEICKGLKKNTGRAVKLG
                     VVHAVLNRLEEKGFTKSRLGEATKARGGKRKRFYTVTSSGKTALVKTKKVRDQIWSTI
                     PKTVISTR"
     gene            2881..5250
                     /locus_tag="VGA21_12780"
     CDS             2881..5250
                     /locus_tag="VGA21_12780"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9651431.1"
                     /translation="MLSNYLKIAFRNIARHKAYSVVNILGLAGSFAITILIIFYSNSV
                     LTYDKFHEDSDNIYFMYRDRATEDGRLDVFDTWYPLAEVTVEEFPVVESGTRMVTIGN
                     TWVELDEKRYEQQLTYADSNFFEVFSWPLLKGDPETVLDDPNSVVISSAIAQRFFGNE
                     DPIGKTLTFGFATQRMVTGVLGEIPSNSSYTFECIVPLNDRQVTQWLGENHWRGSFCE
                     TFIKLRPDADPNALRPQLGLLMEKYVNEQEQGDFLILPIGDLYDKITGRKKYAHILLW
                     VAFGILVIAIINFTNLATAQSLLRVKEVGVRKVLGASKNRLINQFLGESMMMAIISLI
                     TGGLLAELLVNDFGEMVNMNLETNYFQEPVFLALILGIGIIVGLVSGFYPAFFVSRMQ
                     AGKILKGENVKGGRMSFRNVLVMIQFVLAIVLVSGVGIIMRQINFMKNHDLNFDQKNV
                     IVIPLSLRDFRDPSTALPRIISFRNELVNIAGVNTVTGSSSVPGNYRQSFSLFLAEGK
                     MDVTLDWQVAVIDHEFFETYGVKMIEGRSFIQGSSSDFDRGVILNQSALKQIGWETGV
                     GKKLYFPRSREELDVIGVVEDFNVQSLREPVQPLVHYYGGDSARSYRFVSVKINPDSR
                     EQILAEVARTWQRMDFGQELEYYFPAERFRELYATEENVASVLTFSMSFAILIACLGL
                     YALASFSVALKSKEIAIRKVMGATVQSIITAFSRKYLLLVGVAGIPAFVLSWYGMNMW
                     LEDFAYRITIGADIYLFTLLLVILIAFATIAWHALKAGRTNPVDYLREQ"
     gene            complement(5646..>6912)
                     /locus_tag="VGA21_12785"
     CDS             complement(5646..>6912)
                     /locus_tag="VGA21_12785"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LamG-like jellyroll fold domain-containing
                     protein"
                     /protein_id="HEX9651432.1"
                     /translation="DATLILRSGSTLWLQGESLLTIQGGGWLCVEDGASIILQDPASR
                     LILDDGSQMGVHSIWARGHNCLKPDAIEFTGDGGIGPWPGNALTFDGTDDRVTFPNVD
                     ELNLGDFDDFTIEVFFQSYFIGQAPTYQTLLSKRSFRNMSEADGFILGIWPGEPFTGK
                     VFAQFNGTPNYLSESQNLFDGACHYIALTRKENEVTFYVDGISYGSQLSYRSLNSTSP
                     LYAGYDPPEGINFKGEIAEIRFWNLARTSDQIADYENEFITGNETGLMCAWQFNQASG
                     EVALDSSPNLFHANLGNEPEPESFDPMWYLNRECEIPNYFSGNWPDKFVLDAVPDRST
                     KDTIQYEPKLKAWPIPFDREVTLLIESDEDESMELKVYDENGLEVLSTTEYRTNEIIH
                     LEKGLKKGIYVVKARFGNKVKEMKIIKMN"
BASE COUNT         2015 a         1353 c         1407 g         2137 t
ORIGIN      
        1 ttccagggtt ttctcattaa ttatgatttc actttcttca aacttcccgg attttccgct
       61 ataatttctt cccgcaatca acttatgatc atgtagagga atgtagtttt cgtcaacctg
      121 gacaaaatat acaaaaatag aatcattcgg atcctggtat ttcaatgatg ctgaccagaa
      181 attaccaata ctgggaacca ggtttgatcc ggaaatccga atgatttcgg gaatctcgct
      241 aaactctttt cgcattatcg cggcgtcatt accctgaagt ctaacattca aaatattgtc
      301 agtggtgaac ccaaggttaa aggccagcga atgcttgtat tgattgaatc cgataaccgt
      361 agcagttatg aacatgagag agatcgtgta ttgaatggct attaaagcct ttctgaaatt
      421 cagatgtctg aatcctttta ttgaggcagc gtttttaaga acctttacgg gacttattct
      481 gctgaaaaat attgcgggaa gtatcccgga tatgaagccg acaaaaatgg ccaggagaat
      541 aaaaccaatg attacggcgg gactaatttt taatgatact atttctaata actcaggatc
      601 aatggttaga aattgatttc taagcagaat gaaaacaccc gcggaaagca gtagcgctaa
      661 gagtgaaatt actattgatt cagacataaa ttgccagatt acattgccgc ggttcgctcc
      721 gattactttc cttactccaa cctcacggcc tcttcgaata gatcgcgcta tggacagatt
      781 tgtataattg aaagcagctg acaacattac gacaaaagcc aatacaccga caatccacaa
      841 aatgaccgga tccatatttg ctccgtcgtt gttgctaaga ttaggcccaa gaacaaattg
      901 actcatgggc tgcagccata aagatatttt aatgtgctcc agaacagaat tctcagtatc
      961 agataaatta ttgagattcg cctgtaaacc tgtagtttta cccttatcgg gtaataatac
     1021 gtacacgtaa ttcatccaca tgctggtcca tccatataac ctatcgttat tccgattctg
     1081 aatatcaaaa gtggagaagg agccaagcgc ttcgaattta atgtgagaga attcaggaat
     1141 gtctttcagg atgccggtaa ccacataatt agtggtgtcg agtgtcacaa ttttgcccag
     1201 gccactttca atgccaaaaa tctttttaga ggtttgttca gtaagtacaa tggagtaggg
     1261 ctcggccaaa gcattacccg gattgccttc cagcattgga aacgaaaaaa tagataggaa
     1321 agaattgtct gcccacagtc ctctgacagg aattattttt tctccaaaat gtgcatcacc
     1381 gctgaaattt cttctaagaa tgcttacagc ttccgcgccg gccatgtttt cccggagcag
     1441 caggccggtt ttgatggaag tagacgcgta aggccagggg tcgtccttct ggtcagccca
     1501 ggtactgttt acgcggtaaa tcctgtcttt attcatatga aacaaatcat aagattttaa
     1561 gtcggaaata atggaaatca gcaataaccc gacagacatg cttatggcca atcctacaat
     1621 attgatcgct gaaaaaagtt tgtttctgat aatgtttctt cctgatgttt taatgtagct
     1681 tcctaacata atccagtttg ttattaggtt tatgaattga aattttttaa gcgtgtaagg
     1741 tctgaagaat ttgaaggcat ctataacata attaaacctt gcataaaatg gccccctggt
     1801 tcttaggttt tcatcaaagt attcgttcaa atctccctgg aggtcttccg caatatccgg
     1861 tttgcaatac cattcaataa accgggttgc ccatttagga ggtttagcct tggtactttt
     1921 catctggttg aaataacagt tttaggaatt gtactccaga tctgatcacg aacttttttt
     1981 gttttaacca gggcggtttt acctgaagaa gtcaccgtat aaaatcgctt tcgtttgcca
     2041 ccgcgcgctt tggtcgcttc gcccaatctg ctttttgtaa atcctttttc ttccagtctg
     2101 tttaataccg cgtgaacaac ccccaacttc actgctcttc ccgtattctt ctttagtcct
     2161 ttacagattt ccacgctgta ggcatcgtct gataaagagg ctatggttaa caataccacc
     2221 tcttcaaact cacccaggtt cgttcctttc ataaatatta attatttatt acgtaaatgt
     2281 atgtaaaata gttgcaaata gaaaaaatcc ttttactacg ctgtgtaaat ccggccttgc
     2341 caaataacgg taagacgttt tctttaggta tttactacta taaagagggt tattaaactt
     2401 cctataaaaa gaaccataat gaagataaca aaccacaacc gttttatagt atcaataaga
     2461 ttttccatat atcaattcaa ttaaagtctg ttttaaagag ataaagttga cttgatgaat
     2521 tggctatggc ccttcagcag aaataaattc gggaagaatt aaattggata atttaattgg
     2581 aactaatgtc ttaaaacaaa cgggaacttt cataaggata aaagctcttc aaaagtacga
     2641 atagatttag ccggttgaat caaaagttaa ataattattt ttaggttaac cgtaaactaa
     2701 tctttataaa aaaataggaa atcatcacgt tcaattttgg gcttcaaact gaacgactca
     2761 tgtatcaaaa ggaaacacaa tgtttgggca atagttgggg aatatgttgt taatcaatgt
     2821 cttatgattc atggcacatt attggcactt tcaggtgctc tggaattaaa catttaatgc
     2881 atgttaagta attacctgaa aatagctttt cggaatattg ccagacacaa agcctattct
     2941 gtggtaaaca ttctgggctt ggcaggcagt tttgccatta ctattcttat aatattttac
     3001 tctaacagtg tacttaccta tgataagttt cacgaagact ctgacaacat atattttatg
     3061 taccgggaca gagccaccga agatggacgt ctggatgtct ttgacacctg gtatcctttg
     3121 gcggaggtaa cggtggaaga gtttccagtc gttgaaagcg gaacccggat ggttactatt
     3181 ggtaatacgt gggtggaatt agatgaaaag aggtatgagc agcaattaac ttatgcagat
     3241 tctaattttt tcgaagtttt ctcctggcct ttgttgaaag gggacccgga aacggtttta
     3301 gatgatccca attccgttgt tattagcagt gcaattgctc agcgattttt tggaaatgaa
     3361 gatccaattg ggaaaaccct gactttcggc tttgcaactc aacgaatggt tacgggagtt
     3421 ttgggagaaa taccttctaa ttcctcttat acctttgagt gcattgttcc tcttaatgat
     3481 cgacaggtta ctcaatggct gggagagaat cactggcgtg gttcattttg cgaaacattt
     3541 ataaagctgc gaccggatgc tgatcccaat gcactcagac cccagctagg tttgttgatg
     3601 gaaaagtatg tgaatgagca ggagcaagga gattttctta ttctcccaat tggggattta
     3661 tacgacaaga ttacgggccg taagaaatat gcgcatattc ttctttgggt agcatttgga
     3721 attttggtaa ttgcaataat caatttcaca aatctggcaa ccgcccaaag tttgttgcgg
     3781 gtgaaagaag tgggtgtgag aaaagtattg ggtgcaagca agaacaggtt gataaatcaa
     3841 tttctggggg aatctatgat gatggcgatt atatcgctca ttaccggcgg attacttgcc
     3901 gaattacttg taaatgactt tggtgaaatg gtgaatatga atttggaaac taactacttt
     3961 caagaaccgg ttttcctggc tctgattctg ggaattggca tcatagtcgg attggtatcg
     4021 ggattctatc cggctttttt tgtttcccgg atgcaggcag gaaaaatact aaaaggtgaa
     4081 aatgtgaaag gcggcagaat gagtttcagg aatgttttgg taatgattca attcgtgctt
     4141 gctatagtgc ttgtaagtgg agttgggatt atcatgcggc aaattaattt catgaagaat
     4201 catgatctga attttgatca gaaaaatgtt atcgtaattc cactatcctt gcgtgatttt
     4261 cgcgaccctt caaccgcgct gcccagaatt atttccttca gaaatgaatt agtaaatatt
     4321 gcgggagtga atacggtaac cggctccagt tcagtaccgg gaaattaccg tcaaagtttt
     4381 tctctttttc tggcggaagg aaaaatggac gtcactcttg actggcaggt agcggtaatt
     4441 gaccatgaat tttttgaaac ctatggagtc aaaatgattg aaggaagatc gttcattcaa
     4501 ggttcaagta gcgattttga tcgcggtgta attctaaatc aatcagccct gaagcaaata
     4561 ggctgggaaa cgggcgtagg taagaaattg tatttccccc gttccagaga agaattggat
     4621 gtgattggtg tggtggaaga ttttaatgtg cagtcactta gagaacctgt gcagccgctt
     4681 gttcattatt atggaggtga cagcgccaga tcatacaggt ttgtttctgt caagatcaat
     4741 cctgattcgc gggaacagat acttgcggaa gttgccagaa cctggcagcg aatggatttc
     4801 ggtcaggaat tagaatatta ctttcctgca gaaagattca gggaacttta tgcaacagaa
     4861 gaaaatgttg ccagcgtact tactttttcc atgtcgtttg caattctgat tgcctgcctt
     4921 ggactttacg cgctggcatc atttagtgtg gctttgaaat caaaagagat cgcgattaga
     4981 aaagtgatgg gcgccactgt tcaatccatc attaccgcat tttcacgaaa atatttgcta
     5041 ctggtgggag tagccggtat tcctgccttt gttctttcct ggtatggaat gaatatgtgg
     5101 cttgaagact ttgcctatcg aattacgatt ggagccgata tttatctgtt cacactactg
     5161 cttgtaatcc tgattgcttt tgccaccatt gcctggcatg ctttaaaggc aggccgcaca
     5221 aatccagtag actatttaag ggagcaatag tctggaagtt agttatcatt tagaattttt
     5281 tcaggcaaac ttatttctgc cagtgagttc tccttcttta taaaattcgg ttattaagaa
     5341 aactgttaaa tatttctata gtatcagtct gggaaagccg ggtatattca ccctcagaat
     5401 gaacaaaggg tgttatacta atccttttta gattatagtt gatcgtctct gccggacggc
     5461 agcaggccac cactacaatg cttgaatagt aaatcaaata caaggtcatc acttcttcaa
     5521 aaacggtaga actttcatag tagataattt aagtaattat tttagagaac actctcgtag
     5581 attatttata ttgctattac cttctttatt cttgtgattt taatttaatt taatttctca
     5641 aagctttagt tcatcttaat aattttcatc tctttgactt tattaccaaa cctggctttt
     5701 acaacgtaaa tccctttttt caaccccttc tccagatgaa taatttcgtt ggtccgatat
     5761 tcggttgtac tcaatacctc aagcccattt tcatcataga ctttcaattc catggattca
     5821 tcttcatcac tttcaatcag cagagtaact tccctatcaa acggaattgg ccatgccttg
     5881 agtttgggtt catactgaat ggtatctttt gtactccggt caggtacagc atctaataca
     5941 aatttgtcag gccagttgcc ggaaaaataa ttcggaattt cacattctcg gtttaggtac
     6001 cacatggggt caaacgattc cggctccggt tcatttccca gattggcatg gaatagatta
     6061 ggtgaactgt caagagccac ctcaccggaa gcctggttaa actgccaggc gcacataaga
     6121 cctgtttcat ttccggtaat aaattcattt tcataatcag caatttggtc actggttctg
     6181 gcaagattcc aaaaacggat ttcagcaatt tcacctttaa aattaatgcc ttctggagga
     6241 tcatacccag catatagggg cgaagtgcta ttcaagctcc tgtaacttaa ttgcgaacca
     6301 taagaaattc catccacata aaaggtgact tcgttttctt ttcgtgttaa tgctatataa
     6361 tggcaggcgc cgtcaaaaag attctgggat tcactcaggt aattcggtgt accattaaac
     6421 tgggcaaata ctttaccagt gaatggctct cccggccata ttcccagaat gaaaccatcc
     6481 gcctcagaca tgttcctgaa acttcttttg ctgagcaggg tttgataagt gggagcttgg
     6541 ccgataaagt aggactgaaa aaacacctcg atagtaaagt catcaaaatc tccaaggttg
     6601 agttcatcaa cattggggaa tgttaccctg tcatccgttc catcaaaagt caatgcatta
     6661 ccaggccatg gaccaatgcc accgtctcca gtgaattcaa ttgcatctgg tttaagacaa
     6721 ttatgtcctc ttgcccatat tgaatgcaca cccatttgtg atccatcatc cagaatcagc
     6781 cggctggccg gatcctgaag aataatgctg gctccatctt ccacacacaa ccatccgcct
     6841 ccttgaatgg tgagcagact ttctccttgc agccaaaggg tagaaccgga acgtaaaatt
     6901 aacgtggcgt ct
//
LOCUS       DASWHX010000194        12402 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_947331, whole genome shotgun sequence.
ACCESSION   DASWHX010000194 DASWHX010000000
VERSION     DASWHX010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 12402)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12402)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12402
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_947331"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..410
                     /locus_tag="VGA21_12790"
     CDS             <1..410
                     /locus_tag="VGA21_12790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002696756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX9651433.1"
                     /translation="VQIFGTWCPNCMDETKFMANWYEDNKDRGVEIIGLAYENKDDFA
                     YAKARINKMVEKLNVSYDFLFAGSRDNAKASATLPMLNKITAFPTTIFIDKSGKVRKI
                     HAGFSGPGTGHYYIELVEEFNTTIDELVSEEKQ"
     gene            complement(431..1642)
                     /locus_tag="VGA21_12795"
     CDS             complement(431..1642)
                     /locus_tag="VGA21_12795"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020526717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX9651434.1"
                     /translation="MKFSEFNLHENVMQGIDSMGFQEATPVQEKAIPLILEGKDIIGS
                     AQTGTGKTAAFLLPIIHKIALMPEHDDHIKAIVIVPTRELAVQIDQQLTGLSYFTSVS
                     SLAIYGGSDGATFVREMQALKKGADIIIVTPGRFIAHLNLMYVVLNDFKYLVLDEADR
                     MLDMGFHDDIMKIISFLPEKRQNLLFSATMPRRILDLANKILHDPVHISIAISRPAER
                     VLQMAFIVYDKQKLALATHILTSKNLHSVIVFCSTKSSVKSLTRELKKAKLNVEEISS
                     DLEQKQREKVLVDFRNRKINTLVATDVLSRGIDIEDIELILNYDVPNDGEDYIHRIGR
                     TARAESRGVAITLVGENEQSTFAEIEKLLGKPVNKAKIPAQLGSGPEYQPGKRRSVNR
                     KRSFDRKKRKF"
     gene            1786..2880
                     /locus_tag="VGA21_12800"
     CDS             1786..2880
                     /locus_tag="VGA21_12800"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX9651435.1"
                     /translation="MKIRSITWFLTIFLCVWGCQVSKKVDPRILQARVFVEQVRDSSL
                     VPGIALTVIKNGEMIISAGFGYSDLESETPVDPSKTLFRIGSVSKSITGVGLGILMQE
                     EKVNLDTSIYYYLPDFPKKSWDFTTRQVAGHLAGIRHYRNDEFLSNQHYPDVISGLSI
                     FMDDSLLFEPGTEYRYSTYGWSLVSAIMEKASGKPFLEFMQTRVFDALKLRNLQPELK
                     GSQIRNLTGFYQLNENEIVSAPEVDNSYKWAGGGFISTAEDLASFAHKVFFDYALLSE
                     STIDKLITPMTLPDGESTNYGLGWRTGEDDRGHFYVGHSGGSVGGITMLIIYPEEKIV
                     VVMLSNSSNVRYQNAYNKIAWLFMDSEATN"
     gene            complement(2877..4385)
                     /locus_tag="VGA21_12805"
     CDS             complement(2877..4385)
                     /locus_tag="VGA21_12805"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX9651436.1"
                     /translation="MKKLYTILVALLLLVSNSTFCQPSDKNNRIMESFIQKGMIDWEI
                     PGLAVAIVKNGKLVFQEAYGVRELGKKDPVDINTIFSIASTTKAITVACLGILVDEEK
                     LNWNDPVIKHFPEFRLKDPYITKNLTVRDLLTHRAGMPNTDLFWITPNFDKEEIFRRM
                     ELIDPAYSFRSSYTYQNIMYAVAGEVVARVSGVPWHQFVNERIFTPLSMTYSVPLMSE
                     ITSIENKVTPHYIIEGKPETIVTTSTDPVAPAGSIWSSIGDMSKWLAFMLDSANVNGK
                     RLLSASTYSEILKPNHIIPKNSFYSTTALTKPNWTTYGLGWFQHDYRGKMVEFHTGSI
                     GGLIAIVGMIPSEKIGVCVLANMDHAELRHAIMYKSFDLYAFNDNSRDWHQEIFNLYK
                     GSDDAEAVQDQEKEDSRVEGTKTSLEKLDSYAGVFEDGIFGKIDVTFSDEELVFNFNE
                     TEIGRLTHWHYDTFKLIFSKKYWGSSFINFNLNPEGDIEELEFFGITWMKKN"
     gene            complement(4393..5985)
                     /locus_tag="VGA21_12810"
     CDS             complement(4393..5985)
                     /locus_tag="VGA21_12810"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008202362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HEX9651437.1"
                     /translation="MRHSNKIPAFILLIFFWACQTTQKYDLLIQNVTIYDGSGNPPVA
                     GSVAVNGDKIVKVGEFAGISEKIIDGTGHSISPGFIDMHSHLEAIKRLPDAENLVRQG
                     VTTALGGPDGGGPVPFGEYLDTLEIIGVGPNVAFLVGHNTIRRRVMGNEDRDPTGEEL
                     AAMKSMVETAMLEGAFGISTGLKYLPGTFSKVEELIELSKMASAYGGIYTSHLREEGL
                     KLLPAVHEAIEISREAVIPVVLTHHKAIGVPAWGKSVQSLAMVDSARNLGLDIMLDQY
                     PYTASHTGIAVLIPPWALEGSAAEGFEKRVSDPVLRDSIKKAIIYNIMNDRGGRDLRR
                     IQFSRVSWKEDLQGKTLHDWAVEERMEPTVENGAELVIRAQLNGGCGAIYHAMDDADV
                     NRIMQHPQTMIGSDGGLAEIGKLHPHPRYYGTFPRVIGHYARDLALFPVETAIYKMTG
                     LSADRLGIKDRGRIKEGNFADLVIFDVATIIDKSTFTEPHQYPEGIPFVIINGKIAVD
                     NGEFYPVKSGKVLRGPGYLKEN"
     gene            6175..8712
                     /locus_tag="VGA21_12815"
     CDS             6175..8712
                     /locus_tag="VGA21_12815"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009577875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEX9651438.1"
                     /translation="MKPHLLKTILILPGFILLTVSLTVFSQAPRQKSSSEILMDIKKL
                     NVLGNVLYIAAHPDDENTRLIGYLANERLFNTAYLSMTRGDGGQNLVGPEISELLGII
                     RTQELLAARRTDGSEQFFTRANDFGYSKSADETLQIWDREKILSDIIWVIRNFQPDVI
                     ITRFPPDERAGHGQHESSTILAEEAFDLAKEINAFPEQLRYTSVWQPARLLWNTGRFW
                     SQNVNDSDPGVISINTGEYNPYLGKSYPEIAATSRSMHKSQGFGSSGLRGEAIEYLIS
                     KKGSAANEDLMDGIDYSWNRVKGGLRIKARVDKAISSYNVSNPSESVPQLLEIRRELL
                     KLEDAFWRERKIKEINDLIKHCMGLYLEMKSQEPMLTHGDSLKIGVEAINRSQIEAVF
                     SEIHIPSIGISAKVNKSMNYNNRIEEQLSAIIPTTLATTQPYWLIENGTLGVYRVDDQ
                     QLVGKPENNPAIVGEFTVSIAGENMKFSTPVIYKWTDPVKGELYRPLSITPPAFVEIE
                     QKVLMFPNDKPKDVQVRVKSARETIRGELILQVPDGWKVSPASRIVEINSKGEEKVYD
                     FIILPPKDQQIAEVKPIINIDDGTSSNKKLITIEYDHLPTQILFPESSSKIVRVEIER
                     MGDRVGYIMGAGDEIPESLEQIGYSVWKMMDDEVTPENLKQLDAVILGVRVLNTNDRI
                     RFQMPALLGYVKNGGTLVVQYNTTGRLKTNEFSPYPLRISRDRVAEENAQVEILLPDH
                     PALTTPNKISQSDFEGWVQERGLYFPNEWSEEYDALLSSHDQGEDPKNGGLLVAKYGE
                     GYYVYSGYAWFRELPAGVPGAYRLFTNLISLGQKEKPRETKVGRNQK"
     gene            8734..8883
                     /locus_tag="VGA21_12820"
     CDS             8734..8883
                     /locus_tag="VGA21_12820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018479715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651439.1"
                     /translation="MEKPPERNDSQDDELLPSSFGSWKKLYTIVLGTLFILIIIFYLM
                     TTFLS"
     gene            8880..10574
                     /locus_tag="VGA21_12825"
     CDS             8880..10574
                     /locus_tag="VGA21_12825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018479714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HEX9651440.1"
                     /translation="MTTIDWIVLFGTLFFIVGYGVWKTKGSKNIEGYLLGDKSLKWWT
                     IGLSVMATQASAITFLSTPGQAFEDGMRFIQFYFGLPLAMVILSITAIPIYYRLKVYT
                     AYEYLETRFDLKTRSLAAFLFLIQRGLGAGITIYAPAIILSTILGWTLTMTVLLIGIL
                     VIIYTVSGGSRAVSQTQKQQMAVMMGGMILAGIMVVHYLPEHVSFGDAVKVAGKMGKM
                     NVVSLNFDLSDRYNIWSGLIGGMFLALSYFGTDQSQVARYLGGKSITESRLGLLFNGL
                     LKVPMQFLILFIGVMVFVFYQFTMPPVFFNESAKQLLYDTPAKDQVELLEKRHEEIYN
                     QKKSAVNNLIIAMRHGNTLAEEESISDINRAMAESNAIKTEISGIVEEQLPGANPRDT
                     DYVFITFVMENLPVGLVGLILAVIFSAAMSSSSSELNALSSTTTVDIYKRSIRKVGSE
                     RHYLNSSKLFTLMWGLFAIGFALFAARLENLIQAVNIIGSIFYGTILGIFLAAFYFKK
                     IKSNAVFYAALAAQLVVLYCYFRTNIAFLLYNIIGCVLVITLGYIFEMFFRSRNVQ"
     gene            complement(10629..11516)
                     /locus_tag="VGA21_12830"
     CDS             complement(10629..11516)
                     /locus_tag="VGA21_12830"
                     /inference="COORDINATES: protein motif:HMM:NF022588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HEX9651441.1"
                     /translation="MKKINLLVVLLFTAFIANAQSIQTPAPSPFATFSQMVGLTEIKM
                     EYSRPSVKGRNIFGTGDGYLLQYGDIWRTGANSGTQISFSTDVKVNGKEVPAGTYAIY
                     TIPGGDEWKFILYKDLSVGGNTDDYKVEDEMASFVIKAMKPANIVETLTFGINNITPE
                     STATLDLMWENAHISVLIDANADALIVKQIDEAMRNPMAGVGSLYATSANYYYNNNKD
                     LNKALEWMTKACEINKSIFNLETKADIQAKLGKYKEAVATVNELYKAGETVTGGTLNF
                     YNTTLKPRVDTKVAEWNKK"
     gene            complement(11698..>12402)
                     /locus_tag="VGA21_12835"
     CDS             complement(11698..>12402)
                     /locus_tag="VGA21_12835"
                     /inference="COORDINATES: protein motif:HMM:NF022588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HEX9651442.1"
                     /translation="SMKGRDLFAELTPVGQVWRTGANLSTKITISADVELEGKRIPAG
                     TYSFYSIPGEQEWTVIINKKISWGTEYDESQDLERITVPTISTREPLEVFEFYLTPMS
                     DVSGILGFAWGNVKVQMNIKSYDQEKTLASIEEIMSKPEEAKPGDFFAAANYYNTLNK
                     DLKNALAWIDVYINNTENAYWAYRVKAQIQSKLGDNVSAIKTAELAIKIATEQGNDDY
                     VRYTKKEMAGYKSAKK"
BASE COUNT         3613 a         2507 c         2547 g         3735 t
ORIGIN      
        1 tagtacaaat ctttggaacc tggtgtccaa attgtatgga tgaaaccaaa ttcatggcta
       61 attggtacga agataacaaa gatcggggag tagagataat tggcctggcg tacgagaata
      121 aagatgactt cgcttatgca aaagcccgga tcaacaagat ggtagaaaaa ctcaatgtta
      181 gctatgattt tctctttgcc ggcagccggg acaatgcaaa agccagtgca acgctgccga
      241 tgttaaataa gataacggca tttccaacta ctatttttat tgacaagtcc ggaaaagtaa
      301 gaaagataca tgctggattt tcgggcccgg gtaccgggca ttattatatt gaactggttg
      361 aggaattcaa tacaaccatt gatgaactgg tttccgaaga aaagcaataa tattaagcaa
      421 tactcttaat tcagaatttt cgtttctttc tatcaaaact tctcttccgg ttaacactcc
      481 ttctttttcc aggctggtac tccggacctg atcccagctg tgcaggaatc tttgccttat
      541 taaccggttt cccaaggagt ttttcaattt cagcgaatgt gctttgctca ttttctccaa
      601 ctaaagtaat ggccacaccc cttgattcag cccgggcagt gcggccaatt ctgtgaatat
      661 aatcctcgcc atcattcgga acatcgtaat tcaaaatcaa ttcaatatct tcaatatcta
      721 ttccacgaga taaaacatcg gtggcaacaa gggtatttat ttttctgttt cgaaagtcta
      781 caagcacttt ttcccgttgc ttctgttcta aatccgagct aatttcttcg acattgagtt
      841 ttgctttctt caactcccgt gtaagcgatt taacactgga ttttgttgaa cagaaaacga
      901 taacactatg caaatttttg gaagtcagaa tatgcgtagc cagggccagt ttttgtttat
      961 cgtaaacaat aaatgccatt tgcagaaccc gttcagccgg tctggaaata gcaatgctga
     1021 tatgaaccgg atcgtgcaga attttattcg ccagatccag gatgcgcctg ggcatggtcg
     1081 cggagaagag aagattttgt cttttctcag gaagaaaaga aataatcttc atgatgtcat
     1141 catgaaatcc catgtccagc atgcgatccg cttcatcaag aaccaggtac ttaaaatcat
     1201 ttaacaccac atacatcaaa ttcaggtgag caatgaatcg gccaggagtc acgataatta
     1261 tatccgcgcc ttttttcaaa gcttgcatct cacgaacaaa ggtagcgcca tcactgccac
     1321 cgtaaattgc cagggagcta accgaagtaa aataggacag gccggttaat tgttgatcaa
     1381 tttgaacggc aagttccctg gtaggtacaa ttacgatagc tttaatgtga tcatcatgtt
     1441 caggcattaa agcaatttta tgaatgatag ggagaaggaa ggccgctgtt ttgccggtac
     1501 cggtttgcgc ggatccgatt atatcttttc cttccagaat taacgggatt gctttttcct
     1561 gaaccggggt agcctcctga aatcccatgg aatcaattcc ctgcattaca ttctcatgga
     1621 ggttaaattc gctgaatttc aattaccgcg gctttttaat ctaattatga tctcttcatg
     1681 attaatcatc cgcaaaagta aatcactacg acgaaattat cccaacatat tatttaaatt
     1741 ggaaaatttg aagtttattt aagctattaa acaaaatcgg gtagaatgaa aatccgttca
     1801 attacgtggt ttttaaccat ctttctttgt gtatggggat gtcaggtcag caagaaagtt
     1861 gacccaagaa tattacaggc cagggttttt gttgaacaag ttcgtgacag cagcctggtt
     1921 cctggaattg ctcttacagt tattaaaaat ggagaaatga ttatatcagc cggatttgga
     1981 tattccgatt tggaaagtga aactccggtt gatccctcta aaactttatt ccggattggg
     2041 agtgtgagca agtccataac tggtgttggg ttgggaatat taatgcagga agaaaaggtg
     2101 aatctggata cttctatcta ttattacctc cccgattttc cgaaaaaaag ctgggacttc
     2161 acaaccagac aagttgccgg gcatctcgca ggcatccgtc attacaggaa tgatgaattc
     2221 ctgagcaatc agcattaccc ggatgtaatt tccgggcttt caattttcat ggatgacagc
     2281 ctcctttttg agcctggaac agaatatcgt tattccacct atgggtggag cctggttagc
     2341 gctattatgg agaaagcatc aggtaagcca tttttagagt ttatgcaaac ccgggttttt
     2401 gacgcgctga aattaaggaa cctgcaacct gaattgaaag gaagtcagat aagaaatctc
     2461 accggttttt accagttaaa tgagaatgaa attgtatccg ctcctgaggt tgacaatagt
     2521 tataagtggg ccgggggagg tttcatttct actgctgaag atttagccag cttcgcccac
     2581 aaggtgtttt ttgattatgc cttgctaagt gaatccacaa tagataaatt aataactccg
     2641 atgactttgc cggatggcga aagtacaaat tacggacttg gttggcgaac gggtgaagat
     2701 gatcgaggcc atttctatgt tggccatagc ggtggtagtg ttggcgggat cacaatgctg
     2761 attatctatc cggaagaaaa aattgtggtg gttatgctta gcaatagttc taatgtgcga
     2821 tatcagaatg cgtacaataa aattgcctgg ctctttatgg attcggaagc aactaactaa
     2881 ttttttttca tccaggtgat tccaaagaat tccaattcct caatatcccc ttcaggatta
     2941 aggttgaagt taatgaaaga actcccccaa tatttttttg aaaagattag tttgaacgtg
     3001 tcataatgcc agtgcgttaa cctgccaatt tcagtttcat tgaaattaaa aaccaattcc
     3061 tcatcgctga aggtaacatc aatctttcca aaaattccat cttcaaaaac tccagcatat
     3121 gaatcaagct tctctaagga agtcttggtt ccttcaaccc tgctatcttc cttttcttgg
     3181 tcctgtaccg cttccgcatc gtcagatcct ttatacagat tgaaaatttc ctggtgccag
     3241 tctctggagt tatcattaaa tgcataaaga tcgaatgact tatacattat tgcgtgcctc
     3301 aattcagcat gatccatgtt ggccaggaca caaactccaa ttttttcgga gggtatcatt
     3361 ccaacaatgg caatcagacc tccaatactt ccggtgtgaa actctaccat ttttcctctg
     3421 taatcatgct gaaaccagcc cagaccatag gtagtccagt ttggcttggt aagcgcggtt
     3481 gttgaataaa atgaattttt aggaataatg tgatttggtt taagaatttc tgaataagta
     3541 gaggcgctca gcaaacgttt tccattcaca ttcgcgctgt caagcatgaa agcgagccat
     3601 ttggacatgt cgccaatgct tgaccaaatg gacccggctg gcgcaaccgg atctgtgctt
     3661 gtggtaacaa ttgtttcagg cttgccttct ataatataat gcggagttac tttattttca
     3721 atggatgtta tctctgacat caatggaacg gagtatgtca tgctcagtgg cgtaaaaata
     3781 cgctcattga caaactgatg ccagggaact ccgcttactc ttgccaccac ttcccctgca
     3841 acagcataca tgatattttg ataggtatag ctgcttctga aactgtaagc aggatcaata
     3901 agctccattc tccggaatat ttcctcttta tcaaaatttg gagttatcca aaacaaatca
     3961 gtattcggca ttccggctct gtgagtaagt aaatctctaa cggtaagatt ttttgtaatg
     4021 taaggatctt tcaatcgaaa ttccggaaaa tgtttaatca ccggatcatt ccaattcagc
     4081 ttttcctcat ctacaagaat tcccaggcag gcaacagtaa tagctttagt ggtggaggcg
     4141 atagaaaata ttgtattaat gtcaaccgga tccttcttac ccagttcccg aactccgtag
     4201 gcttcctgaa aaactaactt tccatttttt acaattgcta ccgccaggcc cggtatttcc
     4261 caatctatca ttcccttttg aataaaggac tccataattc tgttgttttt gtctgaaggt
     4321 tgacagaaag tggaatttga aaccagtaac aacagcgcga caagaattgt atataatttc
     4381 ttcataatca ttttaatttt cctttaaata tcccggtccg cggagcactt tgcctgattt
     4441 taccggatag aattctccat tatccacagc aatttttcca tttataataa caaagggtat
     4501 gccttcagga tattgatgcg gctctgtaaa agtagattta tcgataattg tcgcgacatc
     4561 aaaaatgacc aggtctgcaa aatttccttc tttgattctg ccgcgatcct taattccaag
     4621 tctgtctgcc gaaaggccgg tcattttata aatagcagtt tcaaccggaa acaaagctaa
     4681 atctcttgcg tagtgtccga tcaccctggg gaaagttccg taatacctgg ggtggggatg
     4741 taattttcca atttctgcca aaccaccatc ggaaccaatc attgtttggg gatgttgcat
     4801 aatgcggttt acatctgcat catccatcgc atgataaatg gcaccgcaac cgccgttaag
     4861 ttgagccctg ataacaagtt cagctccatt ttccaccgta ggttccatgc gttcctcaac
     4921 agcccagtcg tgcagtgtct tgccctggag atcttctttc caggaaactc tggagaattg
     4981 tatccggcgc aagtctcttc cgccgcgatc attcatgata ttataaatga ttgccttttt
     5041 gatgctgtct cttaaaaccg gatcgctgac tcttttttca aatccttcag ccgcgctgcc
     5101 ttccagtgcc caaggcggaa tcaatactgc aattcccgtg tgacttgcag tgtatggata
     5161 ctggtcaagc ataatatcca ggcccagatt tcttgccgaa tctaccatgg ctagcgattg
     5221 aacactttta ccccaggcag gtacgccaat ggccttatga tgggtaagaa ctacggggat
     5281 gacggcctca cgggaaattt caattgcttc atgcacggct ggtagaagtt tcaatccttc
     5341 ttctctaaga tgagaagtat aaataccccc atacgcggaa gccatctttg aaagttcaat
     5401 caattcctca actttagaaa atgttcccgg caggtattta agaccggtgg aaattccaaa
     5461 agctccttcc agcatagcgg tttccaccat tgatttcata gctgcaagct cttccccggt
     5521 tggatctctg tcttcattac ccattaccct tctgcggatt gtattatgac caaccagaaa
     5581 tgcaacgttc ggaccaacac cgattatttc caaagtatcc agatattccc caaatggtac
     5641 aggaccaccg ccatcaggcc cacccagggc cgttgtgact ccttgccgaa ccaggttttc
     5701 agcatcaggc aaccgcttaa ttgcttccag atgcgagtgc atatcaatga agcctggcga
     5761 tatagaatga ccggtgccgt caattatttt ctcagaaata cccgcaaatt ctccaacctt
     5821 aacaatttta tccccattca ccgcaacact acccgcaaca ggtggattgc cggagccatc
     5881 gtaaatagta acattttgaa taagcaggtc atatttctgt gttgtttggc atgcccaaaa
     5941 aaatattaac aggataaagg caggaatttt gttagagtgt cgcatgggta ctgatttaag
     6001 tggttgaaat taagggttaa gatattcttc tcaaattaat tttcaacaaa ccttaaaggt
     6061 aggttttcaa cccgcttaaa tagtcgaaat gttaattcaa atattaaatc ctgaatttcg
     6121 acgcaatgtg cttaactttc agttccaaaa aagttttaat caacatattt taggatgaag
     6181 ccacatttat taaagacaat attaatatta cccggtttta tattgctaac agtttcgcta
     6241 acagtattta gccaggctcc caggcaaaaa agttcttcag agattttaat ggacattaag
     6301 aaattgaatg tattaggaaa tgtgctttat attgcagctc atccggatga tgaaaatacc
     6361 cggttaattg gatacctggc caatgaaaga ttattcaaca cggcatattt gtcaatgacg
     6421 cgtggtgacg gcggccaaaa tcttgtcggc ccggaaatca gcgagttact gggaatcatc
     6481 agaactcagg aattactggc agcccgcaga acagatggaa gtgaacagtt ttttacaaga
     6541 gctaatgatt ttggatattc aaaatcggca gatgaaacgc ttcagatctg ggacagggag
     6601 aaaatactct cggatattat ttgggtgata aggaattttc agccggatgt gatcatcacc
     6661 cggtttcctc ctgatgaaag ggcaggtcac ggccaacacg aatccagcac cattcttgct
     6721 gaagaggctt ttgatcttgc caaagaaata aacgcatttc ccgagcaatt aagatacacg
     6781 tccgtttggc agccggcgcg cctcctctgg aacactggaa gattttggag tcaaaacgtg
     6841 aacgattctg atccgggagt tatctccatt aacacagggg agtataatcc gtacttgggt
     6901 aaatcgtatc cagaaattgc agccacaagc cgaagtatgc ataagagtca gggattcggc
     6961 agtagcgggt tacggggaga ggcaattgag tatcttattt caaagaaagg ttcagcggct
     7021 aatgaagatt tgatggatgg aattgattat tcctggaatc gtgtaaaggg aggtctcagg
     7081 ataaaagccc gggtggacaa agcaattagc agctacaatg tttcaaatcc ttccgaatct
     7141 gttccgcaat tacttgaaat cagaagggaa ttgctgaagc tggaggatgc attttggaga
     7201 gaaagaaaaa tcaaagaaat taatgatttg atcaaacatt gcatgggttt ataccttgag
     7261 atgaaatctc aggagccaat gttgacccac ggtgacagcc tgaaaatagg agtggaagcc
     7321 atcaacagat ctcaaattga agcggttttt tcggagatac acattccatc tataggtatt
     7381 tctgctaagg ttaacaagtc aatgaattac aataatcgaa ttgaagaaca actctcagcc
     7441 attatcccaa caaccctggc aacgacacaa ccttattggc taatagaaaa tggaacttta
     7501 ggtgtttata gggtagatga ccaacaactt gtcggtaaac ccgaaaataa tcccgcaatt
     7561 gttggggagt ttaccgtttc cattgctggt gagaatatga agttttcgac tccggttatt
     7621 tacaagtgga ccgatccggt aaaaggtgaa ttatacagac cactgtcaat tacaccaccg
     7681 gcatttgttg aaattgaaca aaaagtactg atgtttccca atgacaagcc caaagatgtg
     7741 caggttcgtg tgaagtcagc ccgggaaaca atcaggggag aattaatttt acaggttcct
     7801 gatggttgga aggtgagtcc tgcatcccgc attgtcgaaa ttaattcaaa gggtgaggag
     7861 aaagtttacg attttattat tctgccgcca aaagatcagc agatagcgga ggtaaagccc
     7921 ataataaata tagatgatgg aacatcaagc aataagaaac tgattacgat agaatatgac
     7981 catttgccaa ctcaaatttt gtttccggaa tcctcttcga aaattgtaag ggttgaaatt
     8041 gaaagaatgg gcgacagggt tggatatata atgggagcgg gcgacgaaat acccgaaagc
     8101 cttgaacaaa tagggtattc agtttggaag atgatggatg atgaagtaac tcctgaaaat
     8161 ctaaaacaac ttgatgcagt aatattaggt gtgcgggttc ttaatacaaa cgatcggatc
     8221 cgatttcaaa tgcctgcact attagggtat gtgaaaaatg gaggaaccct ggtggttcag
     8281 tataatacta ccggcagatt gaaaactaat gaattctccc catatccttt aagaatttca
     8341 agagaccggg tggcggaaga aaatgcccag gttgaaattc ttttgcctga tcatcctgca
     8401 ttaactacac caaacaaaat ttcccaatca gatttcgagg gttgggttca ggagcgaggc
     8461 ttgtatttcc caaatgaatg gtctgaagag tacgacgcgc ttttatcaag tcatgatcag
     8521 ggggaagacc ccaaaaatgg cggactattg gtagctaaat atggagaggg gtattatgtt
     8581 tatagcggtt atgcctggtt tagagaattg ccggccggag tccccggtgc atacagactt
     8641 ttcactaatc tgatttcttt aggacaaaaa gaaaaaccca gggaaacgaa agttggccgg
     8701 aatcagaaat agaaaaactg aaatttaaaa attatggaaa aaccaccgga acgaaatgat
     8761 tcccaggatg atgaattatt accctcttcc tttggttcct ggaaaaaact ttacaccatt
     8821 gttttgggaa ctctcttcat tcttatcatt attttttacc tgatgactac ctttctttca
     8881 tgactacgat tgattggata gtcttatttg gcactttatt cttcatagtc ggttatggag
     8941 tttggaaaac aaaaggatca aaaaatattg aaggatattt gcttggcgat aaaagtctga
     9001 aatggtggac gatcggactt tcagtaatgg cgacccaggc cagcgccatc acatttctgt
     9061 ccactcccgg tcaggcattt gaagatggaa tgcgattcat ccagttttat ttcggtttgc
     9121 ctttggccat ggtaatcctt tctataacgg ccatacctat ttactatcgg ctaaaagttt
     9181 atacagcgta tgaatacctt gagacaaggt tcgacctaaa aacccgctcc ctggcggctt
     9241 ttctattttt gattcaaagg ggtttgggcg ccggaattac catttatgct cccgccatta
     9301 ttctttctac cattttggga tggacactca ccatgacagt tttgctcatt gggattcttg
     9361 ttattattta tacagtttcc ggtggatcga gagctgtaag ccagactcag aagcagcaaa
     9421 tggccgtcat gatgggtggg atgatactgg caggaataat ggttgtccat tacttgcccg
     9481 aacacgtgtc attcggggat gctgtaaaag ttgcaggaaa gatggggaaa atgaatgttg
     9541 tcagtctaaa ctttgattta tcagacagat ataatatctg gtctggcttg ataggaggga
     9601 tgtttctggc actttcctat tttggaaccg accaaagcca ggtggcacgg tatctgggtg
     9661 gaaaatcaat cactgaaagc aggcttggtt tgctattcaa tggtttgctt aaagttccca
     9721 tgcagtttct aatccttttt attggagtaa tggtatttgt tttctatcaa tttacaatgc
     9781 cgcccgtatt ttttaatgaa agtgccaagc agttacttta tgatacgccg gcaaaagatc
     9841 aggtagagtt attagaaaaa agacatgagg aaatttacaa tcagaagaaa tccgcagtta
     9901 ataatctaat tattgcaatg cggcatggaa atacgttggc ggaggaggaa tctatttccg
     9961 acatcaatcg tgccatggcg gaatccaatg caataaagac cgagatttcc ggaatagttg
    10021 aagaacagct gccaggagca aatccacgag atacagatta tgtatttata acctttgtga
    10081 tggagaatct tccggtggga ttagttggct taattctggc agtaatattt tcagctgcca
    10141 tgagttcttc ctcttcagag ctgaatgctt tgtcatcaac taccacagta gatatttata
    10201 aaagatcaat cagaaaagta ggatcggaac gccattacct caattccagc aaactcttta
    10261 cattaatgtg ggggctgttt gcaattggat ttgcattatt tgccgcacgt ctcgaaaatt
    10321 taattcaggc agtaaatatt atcggttcaa ttttttatgg aactatttta ggtattttcc
    10381 ttgcagcttt ttatttcaag aaaataaaat ccaacgcggt gttttatgca gctttggcag
    10441 ctcaactggt agttctctat tgttatttta ggaccaatat cgcatttcta ctctataata
    10501 ttattggctg tgtattagtc ataacacttg ggtatatttt cgaaatgttt tttagatcca
    10561 ggaatgttca ataaaaaaac accggcttgt ggccagtgtt ttctattatt gtctttataa
    10621 agctgcaatt actttttgtt ccattcagca acttttgtgt caactcttgg ctttaaagta
    10681 gtattgtaga agttcaaagt tccgccggtg actgtttctc ctgccttata gagctcgttg
    10741 acagtggcga cagcttcttt gtattttcca agttttgcct ggatatcagc tttagtttca
    10801 agattgaaga ttgatttatt gatctcgcaa gcttttgtca tccattcaag agctttattc
    10861 aaatccttat tattgttgta gtaataattt gctgaagtag cataaaggga accaacgccc
    10921 gccatgggat ttcgcatcgc ttcatctatt tgcttcacaa tgagagcatc ggcattcgca
    10981 tcaataagta cggaaatatg agcattttcc cacatcagat ccaatgtcgc ggtactttcg
    11041 ggagtgatat tgtttatacc gaaggtcaaa gtttcgacta tattagcggg tttcatcgct
    11101 tttatcacaa aactggccat ctcatcttca actttgtaat cgtcagtatt tccacccaca
    11161 gataaatcct tgtacaggat aaatttccat tcatcgccac cgggaatagt gtaaatggca
    11221 taggtccctg caggtacctc cttaccgtta actttcacat ccgtgctaaa ggaaatttga
    11281 gttccggaat tagctccggt tctccatata tcaccatact gaagaagata cccatctccg
    11341 gttccaaaaa tatttcttcc tttgacactc ggcctggaat actccatttt gatttcagtt
    11401 agccccacca tttgggaaaa ggttgcgaat gggctgggtg cgggagtctg aatggattgc
    11461 gcattagcaa taaatgcggt aaaaagcagt actactagta gatttatttt tttcattttc
    11521 atattgtgtt tgttaggatg ccaaatttaa taaatgaaat gaatatctaa gaatgtattt
    11581 tagatatgat tggcctaatt gaatagaaaa ttgaatgcaa ttttgaattt tatttgattt
    11641 taaggggaag aacaggtttt caaaataaat attgaaaacc tgcctcaagt gcaataatta
    11701 ttttttagct gatttataac ccgccatttc cttttttgta taccgcacat aatcatcatt
    11761 gccttgttct gtcgcgatct taatagcaag ttcagctgtt ttaatcgctg agacattgtc
    11821 tcctaatttt gattgaatct gagctttcac cctgtatgcc caataagcgt tttcagtgtt
    11881 attaatataa acgtcaatcc atgctaatgc atttttaaga tccttattca aagtattata
    11941 gtaatttgcc gccgcaaaga aatctccagg tttcgcctct tcaggcttac tcattatttc
    12001 ttcaatgctc gccaatgtct tttcctgatc ataacttttg atattcattt gaacttttac
    12061 attaccccat gcaaaaccca gaattccaga aacatcggac atgggtgtta aatagaattc
    12121 aaaaacctcc aatggttctc gtgttgaaat tgttggaacg gttatccttt ccaaatcctg
    12181 actttcatcg tactcggttc cccagcttat tttcttattg atgataacag tccattcctg
    12241 ttctccggga attgaataaa aggaataagt acccgccgga atccttttac cttcaagttc
    12301 cacatcagcg gagattgtga ttttagtaga aagatttgct ccggttctcc agacctgccc
    12361 gacaggtgtc agctccgcaa aaagatccct tcccttcatg ct
//
LOCUS       DASWHX010000195         2040 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_587235, whole genome shotgun sequence.
ACCESSION   DASWHX010000195 DASWHX010000000
VERSION     DASWHX010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 2040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2040
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_587235"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(40..699)
                     /locus_tag="VGA21_12840"
     CDS             complement(40..699)
                     /locus_tag="VGA21_12840"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX9651443.1"
                     /translation="MKLILFLCLFLFTTNCFGQYNNIYKESAWEDRDKWQQPERILEA
                     IRVKEGTKVADIGCHQGYFTVKLAGKVGVTGKVYAVDIDSYQLDKLKENLRSRELLDK
                     VEIIKGDYDDPKLPSLTLDAVVIIDSYHEMDENEKILQHIYHSLVPGGRLVLIEPIAP
                     ERESWSRDRQTGKHEIAIRYAREELIFAGFKILKEENPFINRMEEKGDKEWMIIAIRP
                     E"
     gene            875..>2040
                     /locus_tag="VGA21_12845"
     CDS             875..>2040
                     /locus_tag="VGA21_12845"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX9651444.1"
                     /translation="MSRNTIRFVTVLATLSIVGLSIIQIYWAKKVYSLKESEFNLSVS
                     NALLKVSERLCKCEKSTLPANHPVDQLSSNYFVVNINDMIDPVYLDFYLRDELSKRNV
                     ITDFEYGIYDCTSQDMVYTNFVQFDGTPNPELATSTFPTLNSETYYFGVYFPHKYGML
                     VSQMGIWVFLTGVMGLVIIFFGYTLFVILKQKRLSEIQRDFINNMTHEFKTPISTIAI
                     SSEVLRNPDITQNPSRLLNYATIIQNEANRLRQQVERVLQMATLEKEDLGLKKEKVDV
                     HEIINKAISSLEVPLADRHGVVEKELYAQSSEIIADPLHLTNIIYNLIDNSIKYCNEN
                     PKIIVSTRNEKGKFVIEVGDNGIGITPEHQKKVFHKFFRVPTGNIHDVKGFGLG"
BASE COUNT          626 a          411 c          386 g          617 t
ORIGIN      
        1 atttaatatg agtctacctt gaatcgaaaa agtaaaccgt tattcggggc gaatcgcgat
       61 aatcatccac tctttatctc ccttttcttc catacgattt ataaatggat tttcttcctt
      121 aagaatttta aagcctgcaa aaattaattc ttcccgggca taccggatgg cgatctcgtg
      181 ctttccggtt tgccggtccc ttgaccatga ctcccgttcc ggagcgatgg gttcaatcag
      241 caccaatctc cctcctggta caagtgaatg ataaatgtgc tgtaaaatct tttcattctc
      301 atccatctca tggtaagagt cgataataac aaccgcatca agggtaagcg atggtaattt
      361 aggatcgtca taatcacctt tgattatctc aactttatcc agtaactccc ggctccggag
      421 attttccttt aacttatcaa gctggtaaga atcaatgtca acggcataca ctttgccggt
      481 tactcctact ttcccggcca gtttcactgt aaaatatccc tgatgacaac cgatatcggc
      541 aactttggtt ccttctttta ctctaatcgc ctcaaggatt cgctcaggct gctgccactt
      601 gtcacgatct tcccaggcgc tttctttgta gatattattg tactgaccaa agcaatttgt
      661 agtgaataaa aaaaggcaca aaaaaagaat taatttcatg attacaggtt tgaatatttg
      721 ccacaagaaa taggccaaat cagaccaata taccgggcaa ccacttacaa cattccttag
      781 ataattacga acttaactcc ttaattgtta ataacttgtt aaataagact tcacagtatc
      841 ttatttgaat taattttaag ttttactttg aagaatgagc cggaacacta tcagatttgt
      901 aacagtgctg gcaacccttt caattgtggg gttatcaatt attcagatct actgggcaaa
      961 gaaagtttac agcctgaagg agtctgaatt taatctaagc gtcagcaatg cactgttaaa
     1021 agtttcagag cgattgtgta aatgcgagaa aagtaccctg cccgccaatc atcctgttga
     1081 tcaactttcc agcaattatt ttgtggttaa tataaatgat atgattgatc cagtctacct
     1141 ggatttctac cttagggatg aattaagcaa gcgaaacgta attaccgatt ttgaatacgg
     1201 aatttatgac tgcacttccc aggacatggt ttatacgaac tttgttcagt ttgatggaac
     1261 acctaatcct gaattagcaa catcaacatt tcccactctg aacagcgaga catattactt
     1321 tggcgtctac tttccacaca agtatggaat gttggtgagt cagatgggga tttgggtttt
     1381 ccttaccgga gttatgggat tggtgattat cttttttggt tacacgcttt ttgtgatcct
     1441 gaagcaaaaa agattgtcgg agattcagcg cgactttatt aataacatga ctcacgaatt
     1501 caaaaccccc atttctacta tcgccatttc ttcagaagtg ttgagaaatc ctgatatcac
     1561 gcaaaatccg tcgagacttc ttaattatgc aaccataatt caaaacgaag ccaatcgctt
     1621 gcgacaacaa gtggaaaggg tgttgcagat ggctactctg gagaaagagg acctgggatt
     1681 aaaaaaagaa aaggttgatg ttcatgagat aattaacaag gccatttcca gcctggaggt
     1741 tcccctggcg gataggcatg gagtggtgga aaaagaattg tatgctcaat catcagaaat
     1801 tattgccgat ccgctgcatc tcaccaacat catatacaat ttgattgata attccattaa
     1861 gtattgcaat gaaaatccga aaataattgt ttcaacaaga aatgaaaaag ggaagtttgt
     1921 tattgaagtc ggagacaatg gaataggaat tacacccgaa catcaaaaga aagtatttca
     1981 caaattcttc agggttccta ccggaaatat tcatgatgta aaaggatttg gcctgggttt
//
LOCUS       DASWHX010000196        10766 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_2079788, whole genome shotgun sequence.
ACCESSION   DASWHX010000196 DASWHX010000000
VERSION     DASWHX010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 10766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10766
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2079788"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(247..1530)
                     /locus_tag="VGA21_12850"
     CDS             complement(247..1530)
                     /locus_tag="VGA21_12850"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009583083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEX9651445.1"
                     /translation="MQADILLGLQWGDEGKGKIVDFMAPRYKVVARFQGGPNAGHTLE
                     FGDIKHVLHQIPSGIFREDIKNIIGNGVVLDPAAFKKEIDSLSGYGLDLRSNLFISRK
                     AHLILPTHILLDQALEKSKGDKKIGSTLKGIGPTYQDKISRTGLRVGDLLSDNFESKI
                     RERMAVHYEIIAGSEIQFDRKSLEAELMIGIEFLKTFNLADTDYMVNEELQLGNNVLC
                     EGAQGSMLDVDFGSYPFVTSSNTTASGACSGLGISPNKIKDVYGIFKAYGTRVGSGPF
                     PAELFNEIGDSLRKVGNEYGATTGRPRRCGWLDLPALKYSIMLNGVTKLFMMKADVLS
                     VLNEIKVCTEYELKGKVLTDKVPFGIVDDEITPVYKIFPGWKVNLERMRKFDELPGEL
                     QNYITFLEEQLGVPIMLISVGPRREQTLVMDKMLA"
     gene            complement(1534..1878)
                     /locus_tag="VGA21_12855"
     CDS             complement(1534..1878)
                     /locus_tag="VGA21_12855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009583084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX9651446.1"
                     /translation="MKYTHEIIDNILRIRLSGDLIGEDSGMNLVGIANDSLSQDVKLC
                     IIDISDLRYINSSGIGVLITILTKFRNKGGEVYLLHPSENVQKLLIITKLNAIFQIAD
                     NEDEAVQKLKKS"
     gene            complement(1862..2341)
                     /locus_tag="VGA21_12860"
     CDS             complement(1862..2341)
                     /locus_tag="VGA21_12860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013929251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEX9651447.1"
                     /translation="MAAKYENIEEVKRIFITYLEMKNLRKTPERDAILDEIYHTMGHF
                     DVDSLYSTMISKNHRVSRATVYNTLDLLVECDLVSKHQFGRNLAQYEKAFGYKQHDHL
                     ICTDCHQVVEFCDPRIHNIQTTVGDLLQFKVMHHSLILYGSCSKNNCENKEKNEIHS"
     gene            complement(2395..4623)
                     /locus_tag="VGA21_12865"
     CDS             complement(2395..4623)
                     /locus_tag="VGA21_12865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009583086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HEX9651448.1"
                     /translation="MQEVDQEEEKREIIRRYRSLLRKAKPILKEGDARLIKKAFNLAT
                     TAHRDMRRISGDPYIFHPLAVAQICVDEIGLGTTSIVSALLHDVVEDTDIELEEIEKE
                     FGHKVVSIIDGLTKISGVFEYGTSQQAENFRKMLLTLSEDVRVILIKIADRLHNMRTL
                     ESMPREKQLRIASETIFLYAPLAHRLGLYAIKSELEDYYLKYTEVEPYKEIVNKINLA
                     KEKRNRFIKSFIKPLQEELKKLDYDFEIKGRPKSVYSIWNKMKNQNIPLEEIYDLFAI
                     RIILDAEGDLEKQACWQVYSIVTDYYTPNPDRLRDWISTPKSNGYESLHTTVMSQNGT
                     WVEVQIRTKRMDEIAERGYAAHWKYKDSPHSQESGLEKWINKVRDMLEQNDTNAIEFV
                     DDFKSNLFNEEVFAFTPKGELKILPVGATALDFAFEIHTEIGARCLGAKVNQKLVPLN
                     YVIKNGDQIEILTSPKQKPKEDWLRFVVSSKAKTKIKDTLKESKKEAARDGKEIVLRK
                     LKQIKLEPTTETFDKLRTYFNAKTQLDLFYKVGKGLIVPSEIKKFRNKQETADPVKTK
                     NIEDANALKKEIARAKEKKQEILLIGEDMDIVEYRFAKCCNPIPGDDVFGFVTVSEGI
                     KIHRTSCLNAPELLSKHGQRVVKAKWASKHESSFLAGLGIIGTDRVGLINDVTKIISN
                     ELKVNMRSITVDTDTGIFEGNIQLYVNDTRHLDLLIHKLMKVNGVVKVSRFNYLDNEN
                     AP"
     gene            4711..4792
                     /locus_tag="VGA21_12870"
     tRNA            4711..4792
                     /locus_tag="VGA21_12870"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4745..4747,aa:Leu,seq:tag)
     gene            4874..6208
                     /locus_tag="VGA21_12875"
     CDS             4874..6208
                     /locus_tag="VGA21_12875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009583087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEX9651449.1"
                     /translation="MDITLEKTHPLSGSIKITLNEGDYQPKVEEKVKQYAKKADLKGF
                     RKGMVPPSVIKRMYGKSIIVEEVNHLLSHTLQDYIKDNKLTILGEPIPNYDKHAEIDW
                     ENQKNFNFEYEIGLVDNFKLDIKAKKVKAYAIEVGKEEVDKTISNLQNQYGQMTNPEI
                     SEKGDSLFGELKSEDESFSKDLLLEYEKVEKKLQEKMSGVKQEDVLEFDIKKAFKDVE
                     ACAEVLSILPAEAKQLKGAFKFQIKKINRVIKAELSQDFFDKVFGKDTVTTEPEFLDK
                     VKAEVAKGYERESGRFLEQTIVDTLVDNTKINFPEEFLRKWLSVSNEGKVSKEDIAKE
                     FPNYLKELKWSLIKKRITEENQIKVEHADVINKTKELIQAQFGMRMSPELEKNMVSFA
                     DNYLKGNDGENYMRIYNQLQSERIFSFLKTQLSLQEKKIKPEEFQRLVLGQE"
     gene            6259..6927
                     /locus_tag="VGA21_12880"
     CDS             6259..6927
                     /locus_tag="VGA21_12880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009583088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HEX9651450.1"
                     /translation="MNKEEFRKFAVKNKHVSGTAFDDYARQIEDMTQYVIEERPGNFR
                     AIDVFTRLISDRIIFLGMPVDDVIANVVTAQLLFLSSVDPKKDIVMYINSPGGVVTAG
                     LGIYDTMQYVDPEVATICTGLAASMGAVLLAGGANKKRSALPHARIMIHQPSGGMQGK
                     STDMEISLKLIMSLRQELYTILAHHTGKPVKEIEKDSDKDYWMKSDEAKSYGIIDEVL
                     NKKK"
     gene            6931..8151
                     /gene="clpX"
                     /locus_tag="VGA21_12885"
     CDS             6931..8151
                     /gene="clpX"
                     /locus_tag="VGA21_12885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009186393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEX9651451.1"
                     /translation="MAEVTCSFCGRSKKDVDLMISGIHAHICDKCITQAQQILSEETK
                     AKEGETPKFNLIKPIEMKKFLDEFVIGQDEAKKYISVAVYNHYKRLMQKKGEDDITIE
                     KSNILMVGETGTGKTYLARTLAQLLQVPFCIADATVLTEAGYVGEDVESILTRLLQAA
                     NYEVEAAERGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLLEGTQVNVPPQGG
                     RKHPDQKMITVNTENILFICGGAFDGMNKVIAKRINTRPIGFSSDKDRTSVKEDSLLR
                     FVNAQDLKSFGLIPELVGRLPVVTYLEPLSREVLRSILTVPRNALVKQYKKLFEMEGI
                     SLSFTETALDFIVEKAMEFRLGARGLRSICEAIVTDAMFEMPSKEDVKNLNITKPYAE
                     EKFNKSKFTKLKVA"
     gene            complement(8148..9263)
                     /gene="lpxB"
                     /locus_tag="VGA21_12890"
     CDS             complement(8148..9263)
                     /gene="lpxB"
                     /locus_tag="VGA21_12890"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019947137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HEX9651452.1"
                     /translation="MKYYLIAGERSGDLHGSNLVKELKRRDPESEFRGFGGDEMQKTG
                     VTLAVHYAELAIMGVSDVLLGIFKILRLVRKCKQDILDYDPDVLILIDFGAFNLRIAG
                     FAEKKGIKVFYYISPKVWAWNQSRALKIKRTVDRMFVILPFEKDFYKKFDYEVDFVGN
                     PVADAVRSHSINKSFIEINNLDPEKKIIALLPGSRKQELRKMLPVMKQVAERNPQFQF
                     VVAGVNSLPAGLYEIVSEVRNVSVAFEQTYDLLAHADAAIVTSGTATLETALWHVPQV
                     VIYKSDTLNYFAGKLLIKVKYISLVNLIADKEVVKELLHNNVQADVVSKEFLKIASES
                     DQRRFQLKEYEKLSQDLGQQNPSAMAAELMFKYLKSD"
     gene            complement(9277..9705)
                     /locus_tag="VGA21_12895"
     CDS             complement(9277..9705)
                     /locus_tag="VGA21_12895"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011584776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HEX9651453.1"
                     /translation="MGKILVSRKEHFNAAHKLYNPHWSKEKNVEVFGPCANENWHGHN
                     FDLIITVKGEPDQDTGFVVDLKKLSNLIRSRIIDKLDHKNLNLDVDFMQGKMASCENL
                     VFEVWKILEPEIPLLSKNANLHSIRLYETPRNFIEYYGED"
     gene            complement(9780..>10766)
                     /locus_tag="VGA21_12900"
     CDS             complement(9780..>10766)
                     /locus_tag="VGA21_12900"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEX9651454.1"
                     /translation="GNAPFITRIGNSKVAPDLIYLVGSSGVSRSADFGNSWTTTAINN
                     QWSSGTITSTYNIEVSDADYKVIWAGGAMSSAINIHVSSNGGNTFSPVTKYSLRTLGP
                     ISGIATHPYEPNTAYLLFSLARTPKILRTKDLGVSWEDISGYGTDETSSNGFPDVATY
                     CLFVHPVDPNIIWAGTEIGIFESIDDGVSWHFQEGNLPSASIWQIKAVDDKVIVATHG
                     RGLWAAQIDGLQWPENVVVGINKEELSVSANFYPNPASDFINVDFDSPSGKDYVIRLI
                     DAHGRVVRYVPGRSQDGKQSEKIDLDNLSNGIYIVSIEMSGKSESGRIVVIK"
BASE COUNT         3187 a         2139 c         2098 g         3342 t
ORIGIN      
        1 agccttttct cttttaaact catctctttt gactcgtcct ttagagcggt tcgctatcat
       61 tccaattgtt caaagaacgt ccgcattctt gtctccaaaa atacttcgac aacattttct
      121 atgttgttac ttttttgccc caccttctga tcggtttccc ctcttttccc ctcaaaacgg
      181 attgcaaagg taggtggaaa gttaataatt ttcaataaag aggatcagaa aacttgggac
      241 gaaaagctat gcaagcatct tatccattac cagggtttgt tccctcctcg gaccaacgga
      301 aatcagcata ataggaacac ctaactgttc ttccaaaaag gtaatataat tctgcaattc
      361 accaggtaat tcgtcaaact tcctcatcct ttctaaattc actttccaac caggaaaaat
      421 tttgtagacc ggtgtgattt catcatcgac aattccaaag ggcactttgt cagttaacac
      481 tttacctttt aactcatatt cagtacacac cttaatttca ttgagtacgc tcagcacatc
      541 tgccttcatc ataaataatt ttgttacacc gttaagcata atcgaatatt ttaatgcggg
      601 taaatcgagc caaccacacc gtctcggtct tccagtggtg gctccatatt catttccaac
      661 ttttctcaaa ctgtcaccga tctcattaaa aagctcggcc ggaaaagggc cacttcctac
      721 acgggtacca taggccttga aaattccgta tacatcttta atcttgtttg gactgattcc
      781 aagaccggaa caagcacctg aagcggtggt gttcgatgaa gttacaaaag ggtagctgcc
      841 aaaatcaaca tccagcattg atccctgtgc cccttcgcac aaaacattat tgcccaactg
      901 aagttcttca ttcaccatat aatctgtatc agcaagattg aaagttttca gaaattctat
      961 tccaatcata agttctgctt ccaaagactt tctatcaaat tgaatttccg atccagctat
     1021 tatttcgtaa tgaactgcca tgcgttccct gatctttgat tcgaaattat cagacaacag
     1081 atcgccaaca cgcaagcctg tcctggagat cttgtcctga taggtgggtc cgattccttt
     1141 taatgtgctt cctattttct tatctccttt tgatttctcc aaagcctgat ccaggagtat
     1201 atgtgtgggc aaaattaaat gagctttcct tgatatgaat aaattggacc ggagatcaag
     1261 gccataacct gaaaggctat cgatttcttt cttaaaagct gcgggatcaa gtacgacgcc
     1321 attaccgata atatttttta tatcttccct gaaaattccc gatggaatct ggtgcaagac
     1381 atgtttaata tctccaaatt ccaatgtatg gccggcatta gggccaccct gaaatctggc
     1441 aacaacttta taccggggtg ccataaaatc cacaattttt cccttgcctt catctcccca
     1501 ctgaaggccg agtagaatat ctgcttgcat gtatcaggat tttttaagtt tctgaactgc
     1561 ttcatcttcg ttatcggcaa tttggaagat tgcattcagc tttgttatta tcagtaattt
     1621 ttggacgttt tcggaagggt gaagcaaata tacttcaccg cctttattac gaaatttggt
     1681 aagaatggtg attagaactc caattccgct gctgtttata tacctgagat ctgaaatatc
     1741 aatgatacag agtttcacat cctgagaaag tgaatcgtta gcgattccaa ccaaattcat
     1801 tcctgaatct tcgccgataa gatctccgga cagcctaatc ctgaggatat tatctattat
     1861 ctcatgagtg tatttcattt ttttctttgt tttcgcaatt atttttggag caggacccgt
     1921 aaagtattaa agaatgatgc atcactttaa actggagtaa atctccgaca gttgtttgaa
     1981 tattgtgaat ccggggatca caaaattcaa cgacctgatg gcaatcggtg caaattaagt
     2041 gatcatgctg tttatacccg aatgcttttt catattgtgc taaattcctg ccaaactgat
     2101 gtttgctaac taaatcacat tcaaccaata agtccagagt attgtagact gttgcacgac
     2161 ttacccgatg gtttttagaa atcatggttg agtataatga atccacgtca aaatggccca
     2221 tggtatgata gatttcatca agtatagcat cccgctccgg ggttttcctc aggtttttca
     2281 tttcaagata tgtaatgaaa attcgtttta cttcttcaat attttcgtat ttagctgcca
     2341 ttagcctaat tcatattcca aagtaacatt gaataattga aagcaaaaag gagactatgg
     2401 tgcattttca ttatccaaat aattaaatcg gctaactttc actacgccat ttactttcat
     2461 cagtttatga atcaataaat ccagatgccg ggtatcattt acataaagtt ggatattgcc
     2521 ttcaaaaatt cccgtatcgg tatctactgt gattgatctc atatttactt ttaactcatt
     2581 ggaaattatt ttcgttacat cattaattag tcctacccgg tctgtgccaa taattccaag
     2641 ccctgcaaga aaagaacttt catgttttga ggcccatttt gcttttacca cccgttgacc
     2701 atgctttgac agaagttcgg gtgcattaag acaagatgtt ctatgaattt tgattccttc
     2761 gctgacggta acgaatccaa aaacatcatc acccgggata ggattacagc acttagcaaa
     2821 ccggtattcc acaatatcca tgtcttcacc aatcaataaa atttcctgtt ttttttcttt
     2881 agccctggct atttcttttt tcaaggcatt ggcatcttca atattctttg tttttacagg
     2941 atccgcggtt tcttgtttgt ttctgaattt cttaatctca ctaggaacaa tgagaccctt
     3001 tccaacttta tagaataaat ccagctgcgt ttttgcattg aagtaggttc ttaacttgtc
     3061 aaaagtttcg gttgtcggtt ccagctttat ttgctttagt tttcgcaaca cgatctcttt
     3121 tccatccctg gcagcctctt ttttcgattc tttgagggtg tctttgattt ttgttttggc
     3181 ttttgagctt accacaaatc gcagccaatc ttcttttggt ttttgttttg gtgaggtgag
     3241 aatttcaatt tgatcaccat tcttaattac ataattcaat gggacaagtt tctgattaac
     3301 ctttgcaccc aaacaccggg ctccgatttc agtatgaatt tcaaaggcaa agtccagagc
     3361 tgttgctcca acaggaagga ttttcaactc tcctttggga gtaaaagcaa aaacttcttc
     3421 attaaataaa ttggatttga aatcatcgac aaactcaatg gcatttgtat cgttttgctc
     3481 aagcatgtcc ctgactttat tgatccattt ttctaatccg gattcctgag aatgagggct
     3541 atctttatat ttccaatgag cggcataacc acgctcagct atttcatcca ttcttttagt
     3601 ccgtatttgc acttcaaccc aggttccatt ttgactcatc actgttgtgt gaagagattc
     3661 atatccatta ctttttggag ttgaaatcca atcacgcaat cgatccgggt tgggggtata
     3721 ataatcagtt acgatagagt aaacttgcca gcatgcttgt ttttccaggt cgccttcagc
     3781 atcaagaata attcggatag caaagagatc ataaatttct tccaggggaa tattttgatt
     3841 cttcatcttg ttccaaatag agtatactga ctttggtctg cctttaattt caaaatcata
     3901 gtcaagtttt ttcagttctt cctgaagagg tttgataaaa ctcttgataa acctgttacg
     3961 cttttctttt gccaggttaa ttttgtttac aatttcctta tatggctcaa cttcggtgta
     4021 tttgaggtag taatcttcca attccgattt gatagcgtag agtcccaatc gatgggcaag
     4081 tggagcataa agaaagatag tttcagaagc tattcttaac tgtttctccc tgggcattga
     4141 ttccagagtt ctcatattgt gcagacggtc tgcaattttt atgagaatta cccgaacatc
     4201 ttctgaaagg gtgagcagca tttttctaaa attctctgct tgttgtgaag ttccgtattc
     4261 gaatacacct gatatttttg taaggccatc aataatggaa acgaccttat gtccgaattc
     4321 cttttcaatt tcttcaagct caatatcggt atcttcgacc acgtcatgta ataaggctga
     4381 tacgattgaa gtggttccaa ggccaatttc atccacgcaa atctgggcta cggcaagggg
     4441 gtgaaagatg tatgggtcgc cggaaattct ccgcatatcg cgatgggctg ttgtagcgag
     4501 attaaaagct tttttgatta atcgggcatc gccttctttc aagataggtt tagcttttct
     4561 gagcaaagaa cggtagcgcc gtattatttc tcttttttct tcttcctgat ctacttcctg
     4621 gatttccttt acttctgcca ttttctaaaa acggatgcct gaattgttcg tattgaaata
     4681 ttatgtaaat ttgcccctat tttttccaaa gcggatgtgg cgaaattggt agacgcacta
     4741 gacttaggat ctagcgccgc gaggcatggg ggttcgagtc cctccatccg cacagtaaac
     4801 cctcgatttg tgaatttaac gattcggagc gagggcatca attttttcag aattgtctat
     4861 taaaccaaca gctttggaca tcactttaga aaaaactcac ccgttatcgg gttcaattaa
     4921 aattacacta aacgaagggg attatcaacc caaagttgaa gaaaaagtaa agcaatacgc
     4981 caagaaggcg gatcttaaag gctttcgaaa aggaatggtg cctccctcgg taatcaaaag
     5041 aatgtatggc aaatctataa tcgtagagga agttaatcac ctattatcgc acaccctaca
     5101 ggactacatt aaggataata agttaaccat ccttggagaa cctattccta actatgataa
     5161 gcacgcggaa attgactggg aaaatcagaa gaatttcaat ttcgaatacg agatcggtct
     5221 tgtagacaat ttcaaactgg acataaaagc caagaaagtg aaggcttatg caattgaagt
     5281 gggtaaagag gaagttgata aaacaatttc caatcttcaa aaccaatatg gacagatgac
     5341 caatcctgaa atcagcgaga aaggagatag tcttttcggt gaactaaaga gcgaagatga
     5401 atcattttcg aaagatctat tgctggagta tgagaaagtt gagaaaaagc ttcaggaaaa
     5461 aatgagcggt gtgaagcagg aagacgtatt ggaatttgac atcaagaaag cctttaaaga
     5521 tgttgaagca tgcgctgagg ttttatcaat acttcctgcg gaagccaaac aacttaaagg
     5581 agcgttcaag tttcaaataa agaaaattaa cagggttata aaggcggaat taagccagga
     5641 tttttttgat aaggtatttg gcaaggatac cgtgactact gaaccggaat ttttagataa
     5701 agtgaaggcg gaagttgcaa aaggttatga gcgggaatca gggcgatttc tggaacaaac
     5761 aattgtggat accctggttg acaataccaa aataaatttt ccggaagaat tcctcaggaa
     5821 gtggctttcg gtgtctaatg aagggaaggt ctccaaagag gacattgcta aggagtttcc
     5881 caattatctt aaagaactta agtggtcttt gataaagaaa cgaattactg aagaaaacca
     5941 aattaaggtt gagcatgcag atgtaataaa caagacgaag gagttaattc aggcacaatt
     6001 tggaatgaga atgtctcctg aactggaaaa aaatatggtt agttttgccg ataactatct
     6061 gaaagggaat gatggtgaaa attacatgcg tatttataac caattacagt cggaacgcat
     6121 tttcagtttt ctgaaaacgc aacttagctt acaggagaaa aagattaaac ctgaagaatt
     6181 tcagaggctc gtattggggc aggaataaaa atgccttata ttggcattaa tcatctgttt
     6241 tatagcacta tataattaat gaacaaagaa gaatttcgaa aattcgcggt taagaataag
     6301 catgttagtg gaaccgcgtt tgacgattat gctcgtcaaa ttgaagacat gactcaatat
     6361 gtgattgagg aacgcccggg taatttcagg gcaattgatg tatttacgcg gttaatcagt
     6421 gacaggatta ttttccttgg tatgccggta gatgatgtta tagctaatgt tgttacggca
     6481 cagcttttat ttctttcttc tgttgaccca aagaaagata ttgtaatgta catcaacagt
     6541 ccgggcggcg ttgttacggc aggattgggt atttatgaca ctatgcaata tgttgatccg
     6601 gaagtagcca ccatttgcac aggtcttgcg gcttcgatgg gggcggtact gttggcaggc
     6661 ggcgccaata aaaaaagatc agcactgccg catgccagga taatgattca ccagccatca
     6721 gggggaatgc agggtaaaag tacggacatg gagatatctc ttaaattgat aatgtcatta
     6781 aggcaggaat tgtataccat tcttgcccac catacgggaa agcctgtgaa ggaaattgag
     6841 aaggattcag ataaggatta ctggatgaaa tctgatgaag ccaagagtta tggtataatt
     6901 gacgaggtat taaataaaaa aaagtaggtt atggctgaag ttacctgttc attttgcggg
     6961 aggagtaaga aagatgttga tttaatgata tcaggcatac atgcgcatat ttgcgacaag
     7021 tgcataacac aggctcagca aattctttct gaagaaacca aggcgaagga gggtgaaact
     7081 ccaaagttca atttgatcaa gccaattgaa atgaaaaaat ttctggatga gtttgtgatt
     7141 ggacaggatg aagccaaaaa atatatttct gtcgcggttt acaatcatta caagagattg
     7201 atgcaaaaga aaggtgaaga tgatattaca attgagaagt caaacatttt gatggtgggt
     7261 gaaaccggaa caggaaaaac ctatttggca agaactcttg ctcaactttt acaagtacct
     7321 ttttgtatag ccgatgctac tgtgcttacc gaggcgggtt atgtagggga ggatgttgaa
     7381 agcattctta cgcggctgct tcaggcagcc aattacgaag tggaagcggc agaacgaggc
     7441 attgtttaca ttgatgaaat tgacaagatc tcgagaaaat cagataatcc ttcaattacg
     7501 cgagatgtta gcggggaagg tgttcagcag gccttgctaa aactgctgga aggaacacag
     7561 gttaatgttc ctccccaggg tggcagaaaa catcccgatc agaagatgat cacagttaat
     7621 acggagaaca ttttatttat ttgtggtggt gcatttgacg ggatgaataa agttatagct
     7681 aaaagaatca acacccgtcc tataggcttt tcctctgaca aagacagaac atccgttaag
     7741 gaggacagtt tgcttcgttt tgtaaatgcc caggatctta aaagttttgg gctaattccg
     7801 gaactagtag gaaggttgcc tgttgttacc tatttggaac ctttaagcag ggaggttttg
     7861 agaagcattt taactgtacc aagaaatgcg ctggtaaaac agtacaagaa attgttcgaa
     7921 atggaaggca taagtctttc ctttacagaa actgcattag attttatagt tgaaaaagca
     7981 atggaattta ggcttggtgc aagaggacta agatcaattt gcgaagcgat tgtcaccgat
     8041 gccatgtttg aaatgccaag caaggaggat gttaagaatc taaacataac aaaaccctac
     8101 gcggaggaga agttcaataa gtccaagttc accaagctaa aagttgctta atcacttttc
     8161 agatatttga acattaattc ggcagccatc gccgaaggat tttgctgacc caaatcctga
     8221 cttaattttt catactcttt aagctgaaat cttctttgat cggattcact ggcaattttt
     8281 agaaactcct ttgatactac atctgcctgc acattgttgt gcaatagttc cttaactact
     8341 tctttgtcgg caataagatt aaccaggcta atatatttga cttttatcag cagttttccc
     8401 gcaaaataat taagcgtatc tgatttataa attactactt gcggaacatg ccaaagagca
     8461 gtttctaaag tagctgttcc ggaagtgact attgcggcat ccgcgtgggc caacaaatca
     8521 tacgtttgtt caaaggccac agaaacattc ctgacttctg aaacaatttc atataaaccg
     8581 gctggcagtg aatttacacc agccaccaca aattgaaatt gagggtttct ctctgcaact
     8641 tgcttcatga cgggaagcat tttgcgcaac tcttgttttc tactgcctgg aagcagcgcg
     8701 attatcttct tttccggatc aaggttattt atttctataa aagatttatt tatagaatga
     8761 cttctcacag catctgctac cgggtttcct acaaaatcca cttcgtaatc aaactttttg
     8821 taaaaatctt tttcgaaagg aaggattaca aacatcctat ccactgttct ttttattttt
     8881 aatgccctgc tctgattcca ggcccaaacc tttggagata tgtaatagaa tactttgatt
     8941 ccttttttct cagcgaaccc tgcaatgcgc aaattaaaag cgccaaaatc gatcaatatc
     9001 aaaacatctg gatcataatc cagaatatcc tgtttgcatt ttcttactaa tctgagaatc
     9061 ttaaaaatgc caagcaacac atcagaaact cccataattg ctaattccgc atagtggacc
     9121 gccagggtaa ctcctgtttt ttgcatttca tctccgccaa accccctgaa ttcagattca
     9181 ggatctctgc gttttaattc tttaaccaga tttgagccat gcagatcgcc cgatctttca
     9241 cccgctataa gataatattt catttacaat gcgggtttaa tcttcgccat aatattcaat
     9301 gaagttccgg ggggtttcat acaatcgaat ggagtgcaga tttgcatttt tactcaacaa
     9361 cggaatctca ggttcaagaa ttttccagac ttcaaaaaca agattttcac aactcgccat
     9421 tttgccctgc ataaaatcga catccagatt cagattttta tggtcaagtt tgtcaataat
     9481 cctggatcga atcaggttac tcaatttttt caagtcaacc acaaatcctg tatcctggtc
     9541 tggctctcct ttcaccgtaa ttataagatc gaaattatgg ccgtgccaat tttcattggc
     9601 acaggggcca aaaacttcca cgtttttttc tttagaccaa tgcggattgt acagcttgtg
     9661 tgccgcatta aaatgttctt ttctgcttac cagtatttta cccatgatac caggctacaa
     9721 attctttggc aaatatagtt aattccgagg gtttggattt ggttggtatt ccaggaggtt
     9781 tatttaatta ccacaatcct tccggattca ctttttcctg acatttctat actgacgata
     9841 taaatcccat ttgatagatt atcgagatct attttttcgc tttgttttcc gtcttgtgaa
     9901 cgacccggta catacctgac aaccctaccg tgcgcatcaa tcaacctgat aacataatct
     9961 tttccggatg gtgaatcaaa gtcaacgtta ataaaatcac tggcagggtt gggatagaaa
    10021 ttcgcgctaa cagacagttc ttccttatta attcctacca ctacattttc aggccattgc
    10081 aagccatcaa tctgtgcagc ccagagtccg cgaccgtggg ttgctactat cactttgtca
    10141 tctaccgcct ttatttgcca gatggatgct gatggaagat ttccttcctg gaaatgccag
    10201 cttacgccat catcgatgga ttcaaaaatc ccaatttcgg taccagccca aatgatgtta
    10261 ggatcaacgg ggtgaacaaa taaacaatag gtggcaacat ccggaaaacc attgctgctt
    10321 gtttcatcag ttccgtagcc actgatgtct tcccatgata ctcctaaatc ttttgttcgt
    10381 aaaattttcg gcgttcgagc taaagagaat agcaagtaag cggtgttggg ttcataaggg
    10441 tgagtagcaa tacctgatat tggccctaag gtacgcagtg aatattttgt taccggactg
    10501 aatgtattgc ccccattaga ggaaacatga atatttatgg ctgagctcat cgctccacct
    10561 gcccagatca ctttgtaatc cgcatctgaa acttcgatgt tgtatgtaga agtaatagta
    10621 ccactgctcc actgattatt gatggctgtt gtagtccagg aattgccgaa gtcagcagat
    10681 cttgaaactc ctgaagaacc taccaggtaa attaagtccg gagcaacttt tgaattgccg
    10741 attcgagtta taaatggtgc attacc
//
LOCUS       DASWHX010000197         8804 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_1317881, whole genome shotgun sequence.
ACCESSION   DASWHX010000197 DASWHX010000000
VERSION     DASWHX010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 8804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8804
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1317881"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(314..763)
                     /locus_tag="VGA21_12905"
     CDS             complement(314..763)
                     /locus_tag="VGA21_12905"
                     /inference="COORDINATES: protein motif:HMM:NF025160.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HXXEE domain-containing protein"
                     /protein_id="HEX9651455.1"
                     /translation="MKTFSPNQTAMLLPLSLLAHQLEEYFGKFPIWYSNLLDAQLSDQ
                     DFIMINVIGLLLISGFSLSYFFNKNNLILVALGTLIFVNGIIHLVLSIFTLSYSPGVI
                     SGVILFLPLGTSIYLRILPKLEEKEKVAAISIGILLLLSVSMIAMNI"
     gene            complement(827..2443)
                     /locus_tag="VGA21_12910"
     CDS             complement(827..2443)
                     /locus_tag="VGA21_12910"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEX9651456.1"
                     /translation="MKIPYYLLLPALALGGACDPKSEPEDLSQLLELVAGDSLSAHIK
                     YLASDELKGRLPGTPEYGLAIDYVIGKFKEMEIEPAGERGEYIQMLTIRNSRISEGNS
                     FMEWNGKMLSANEDYYFIGNLNEVTSELPETEIVFAGYGINAPNYDDFSLVDVENKVI
                     LVLIGSPESLQSSERAHFSNWETKYRKAADKGVVGILFINSNPSIDSTFAARANSASQ
                     RGVFGVTREDGEAFGRRAFPGKLKFSALLNSASMASKELNAAISDYKENKIVGQSIGS
                     IRVKTESIFSEFQSANIVGKIEGSDNTLKKEYVIHTAHLDHVGIGKPVEGDSIYNGAH
                     DNASGVASLLEIARLYQSLERKPKRSILFVMVTAEEMGLLGSDYFTTYPTVPIESIVA
                     NVNTDMPTLIAPLLSIEPLGAEHSTIMNEVKNAAEILSLEIMPDHMPDEVRFVRSDQY
                     NFIKKGIPALHVKYGLKSTNPDEDLSKAIRNYTRNVYHKPSDEWNDLFDFEAGEVYVK
                     CNFLISYFIANNAERPAWNTDSFFNPLNQR"
     gene            complement(2555..2749)
                     /locus_tag="VGA21_12915"
     CDS             complement(2555..2749)
                     /locus_tag="VGA21_12915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010852279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HEX9651457.1"
                     /translation="MKKGTVKFFNASKGFGFIKDSESEKEYFVHISGLIDQIKQDDEV
                     VFELQEGKKGLNAANVKLAS"
     gene            complement(2844..4568)
                     /locus_tag="VGA21_12920"
     CDS             complement(2844..4568)
                     /locus_tag="VGA21_12920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013408534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 family protein"
                     /protein_id="HEX9651458.1"
                     /translation="MIRNVLSIYLVVFILSGSKLYSQSPDLYAQTSEVNNIMVQFRAD
                     YGSLSRFYFMENTFERIQRFKDLNQEYLGKLSALDFDELNVSSRVDYILFSRDLNDNM
                     YQLQAQESEMNQIKEWITFATPVYELEKKRRRGLSMNGSEVARVLNNTLETLQAGLQK
                     LNDTQPFDSRLTNRAEAAIKDVQRGLEHLYEFYNGYDPDFTWWVEKPFVKLDTMISRY
                     GRLMKSKAKLITTQQDDGSGIVGQPIGREEIIRQLNQEMIPYTPEELIEIANREFAWC
                     DAEMLKASTELGFGKDWHAALEKVKNTYVPEGEQPAAILKLYNESIDFLKQNDLLTIP
                     PVAEESWRMIMMTPRRQLVNPFFTGGEVLSISYPTNTMDHDDKLMSMRGNNPHFSRST
                     VHHELIAGHHLQFFMNSRYKTYRRFRTPFWTEGWALYWELLLYDLKFPQSPEDRIGML
                     FWRMHRCARIIFSLNYHLGKWTPQQCIDFLVDRVGHEKANAEGEVRRSFVGGYSPLYQ
                     VAYMLGGLQFWALKKELVDSGEMTYKEYHDAVLQENSLPVEMIRATLTNQSLNKDFKT
                     RWKFYNLR"
     gene            complement(4593..5453)
                     /locus_tag="VGA21_12925"
     CDS             complement(4593..5453)
                     /locus_tag="VGA21_12925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009053530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HEX9651459.1"
                     /translation="MEGIIDYFAEIPSVYRTIFFIVGITFFWVLEGTIPLFSFEYKKI
                     VHALKNLTLNALQLLIGLGLAGALLLASDFTAQNQFGILYLLGLPLWIHVILGVLLLD
                     FIGAYFIHWLEHRVKWMWKFHLIHHTDTNVDVTTGLRHHPGETIFRITFTLLAVLIAG
                     VPMGVIIIYQTLSVIFAQITHANIKVPKGPDKILSWIFVTPNMHKVHHHYTQPYTDTN
                     YGNIFSIWDRIFRTFAEKDVDSLVYGIDTHMNPKENDDLINLLEIPFQKYRAPKGSKF
                     YEEEIKENLA"
     gene            complement(5455..6144)
                     /locus_tag="VGA21_12930"
     CDS             complement(5455..6144)
                     /locus_tag="VGA21_12930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651460.1"
                     /translation="MKRIISATILLLLPFLISAQELYRTTMVRAAPGKLLELIDAIKK
                     KAEVEKKDLHAIRHSQGDHWDLLIMETIDDYDDTGLLLTTGSRGVLDDPMDHLISWKE
                     ELYVTGPTHREFRRTFNNSDFFHVEIFVALSGQRKQLYDERVMENKYLEELSRDPNQI
                     FVKTQGAKWDMFTMGYYKDLKDYANSAQSSDKKEEAAAKAAGFTSAGSIGAYLRNFIT
                     EHHDTLAVKVK"
     gene            complement(6165..7019)
                     /locus_tag="VGA21_12935"
     CDS             complement(6165..7019)
                     /locus_tag="VGA21_12935"
                     /inference="COORDINATES: protein motif:HMM:NF024628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HEX9651461.1"
                     /translation="MCRLLAYLGRPVVLDKFLFQPKNSLINQSFHALEIEEPLNGDGF
                     GVGWYHKEIAQDPAVFVSIYPAWNNRNLRYMAPKIMSDCLVAHVRAASEGDVSESNCH
                     PFHFKNFLMAHNGGVENFSRIKRSVHKKLSDPLFHWIQGQTDSEHLFALVLDNLMKKD
                     RIDADTISQSFEETIAFLLEAMHEQKIDDPAYLNMVMTDGERLVALRYASDKTVKPLT
                     LYYSEGSRYECQEGVCQMIPAREGEKSVMVVSEKLTDIVDDWRMIPENNFILVYKDLS
                     VKIRPVSL"
     gene            7113..8513
                     /locus_tag="VGA21_12940"
     CDS             7113..8513
                     /locus_tag="VGA21_12940"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HEX9651462.1"
                     /translation="MLVTFVIGYILVTLGIGFWYARRVKSSADFVSAGRQLPLILNSF
                     ALFALWYGSETIFGASAVFMEEGLLGVIEDPFGAALCLILFSVVFARRLYRMNLFTLG
                     DLFRNKYGRKVEVIASFFMIISFFGYVAAQLVALGLLIHSLMGIGLETSIIASAIIVG
                     TYTVVGGMWAISVTDFLQSVVIILGLGWVTYTLVSEAGGLGLVIESAPAGFYRFIPEA
                     EPLSWIEYFGAWSVIGLGSLASQDVFQRVNAAKSEKSAVLAGYLGGGIYLLFSMFPLI
                     IILSIRKLYPELQVSDTQQSIPEVILLHSSLPVQIFLFGALISAIFSTCSGAILAPAS
                     LMAENVIKPLWGNKLSEKRFLIILRISVIVITLTGMMMALGSKNIYELVGESSILGLV
                     SMLIPMTAAIFWQRANALGAISSMILGFIFWLFAEHIFSTSIPALFYGMVGGLIGLFA
                     GNFGSKSINKKIEGEH"
     gene            complement(8475..>8804)
                     /locus_tag="VGA21_12945"
     CDS             complement(8475..>8804)
                     /locus_tag="VGA21_12945"
                     /inference="COORDINATES: protein motif:HMM:NF016213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HEX9651463.1"
                     /translation="FLITWGILAGAVTLLDYVIPVYGTKRFGGSRYGIMGSIIGLLLG
                     IFFFPPFGIIIGPLIGALLGELYAGQKGKNAMRSAIGSFAGFLFGTFLKLIVSLVLTF
                     YFFVNAF"
BASE COUNT         2520 a         1897 c         1695 g         2692 t
ORIGIN      
        1 gtttgttttc ccataactat agataattca ttgcatttca aaagtacttt gtatttcaaa
       61 gtaaagcaaa gaaattacct aatactattt tcctgggcat tagattttac cgttggaatt
      121 tgttaataag gaattaattt agaaaataac cttgctaaat cttcaggagg gattcagatg
      181 cttattaata acccgtatca cccaaagaga ccattcagta ttgccaaaat ataaggttga
      241 taaataccac cggacagtaa tgatggcatt cccgggaaac ccaggtgcag gatagcaaaa
      301 atgccaacac ttttcaaata ttcatcgcaa tcatgctaac gctaagtaac aaaaggatgc
      361 caattgaaat ggctgcgacc ttttctttct cttccaattt aggcagtata cgcaaataaa
      421 tactggttcc taaggggagg aaaagaataa ctcctgaaat tacgcccggt gaatacgaaa
      481 gggtaaaaat actaagcacc aaatggataa taccgttcac aaaaattagt gttcccagcg
      541 cgactaaaat caggttattt ttattgaaaa aataagaaag tgaaaagccc gagataagga
      601 gcaagccaat aacattgatc attatgaaat cctgatcgga aagctgtgca tccagaagat
      661 tggaatacca aattggaaac ttaccaaaat attcttccag ttgatgagct aaaagactta
      721 agggtagcaa catcgccgtc tgatttggtg aaaaagtttt cataactgat gcggttatta
      781 ttccatgtta acacttcaat ggtaaaatat cagatacttc ttcacactac ctttgattca
      841 gaggattaaa aaaactgtcc gtattccatg ccggtctttc agcgttgttg gctataaagt
      901 agctgattaa aaagttgcat ttcacataga cctcccctgc ttcgaaatca aacaggtcgt
      961 tccactcatc ggaaggtttg tgataaacat ttctagtata attacgtata gccttactta
     1021 gatcttcatc tggattggta gactttaacc catactttac atgcaaagcg ggtattcctt
     1081 tcttaataaa attatattga tcgctcctca caaaacgcac ttcatccggc atatgatcgg
     1141 gcattatctc caatgaaaga atctctgccg catttttaac ttcattcata atggtagaat
     1201 gctccgcacc taatggttct atggaaagta atggcgcaat taatgtcggc atatctgtat
     1261 ttacattggc tacaatactt tctattggca cggttggata tgtagtgaaa taatcagagc
     1321 ccaaaagacc catttcttcc gccgttacca ttacaaataa aatggatctt tttggctttc
     1381 tttctaatga ttggtaaagc cgggctatct caagaagact tgccacaccg gaggcattgt
     1441 catgagctcc attgtaaatg gaatctccct caacaggttt gccaattccc acatgatcca
     1501 gatgggcagt atgaattaca tactcctttt taagcgtatt atcactacct tctatcttgc
     1561 ccacaatatt cgcgctttga aactcagaga aaattgattc ggtttttacc ctgatacttc
     1621 caattgactg tcccacgatc ttattttcct tgtaatcaga gattgccgcg tttaattctt
     1681 tggaagccat ggaagccgaa ttcaggaggg cagaaaattt taatttgcct ggaaatgcac
     1741 gtctgccaaa cgcttcaccg tcttcccggg taactccaaa gacacctctc tgagaagctg
     1801 agtttgccct ggccgcaaac gtggaatcaa tggatggatt cgaattgata aaaagtattc
     1861 caacaactcc tttgtctgcc gcttttctat attttgtttc ccaattagaa aagtgcgcac
     1921 gttctgaaga ttgaagagat tcaggactcc cgatgagaac aagtattact ttattttcaa
     1981 catcaaccaa agagaagtca tcataatttg gggcattaat tccataccct gcaaatacga
     2041 tttctgtttc aggtaactct gaagttactt cattcagatt tcctataaaa taataatcct
     2101 cattggctga aagcattttt ccattccatt ccatgaaaga attcccttca cttatcctcg
     2161 aattccttat ggtcagcatt tggatatatt cacccctctc ccccgcgggt tcaatctcca
     2221 tttctttaaa tttcccaata acgtaatcga tagccaaacc atattcaggc gtacccggca
     2281 atctgccctt taattcatcg gaggccagat actttatatg ggcggataag gaatcccccg
     2341 caacaagttc caaaagttgt gacaaatctt ccggttctga ttttggatcg caggctccgc
     2401 caagagctaa cgcgggtaat aacaaataat acggtatttt catcttaatt agcaattcat
     2461 gaatggttga aagtaataat taaattcgat ttcatccaat aaaaaagccc ctcccaaaat
     2521 tgggaagagc ttaatttcct taaacgaagg gttcttagga ggctaattta acattagctg
     2581 cattcaaacc tttttttccc tcctgaagtt caaaaaccac ttcgtcgtct tgttttatct
     2641 ggtcgattag accagaaata tggacaaagt actctttttc actttcggaa tctttaatga
     2701 agccaaaacc ttttgacgca ttaaagaact taactgttcc ttttttcatt ttgataatta
     2761 aactaaatat taggtccaaa gttgcataaa ttttgccaaa tctgatcttt ccgattgcat
     2821 tatttgaaga aaatacttac ggtttatctt aaattataga atttccatcg cgttttaaaa
     2881 tccttgttta atgattgatt ggtaagtgtc gccctgatca tttcaacagg taaactattt
     2941 tcctgcagaa ccgcgtcgtg atattcttta tacgtcattt ctcccgaatc caccagttcc
     3001 tttttcaggg cccaaaattg cagaccaccc aacatatagg ctacctggta aaggggtgag
     3061 taaccaccta caaaagacct tcgcacttca ccttcggcgt ttgctttttc atgaccaaca
     3121 cggtccacca gaaaatcgat acattgctga ggagtccatt tacccagatg gtaattcagg
     3181 ctgaaaataa ttcttgcaca gcgatgcatt ctccaaaaaa gcatcccgat ccggtcttcc
     3241 ggtgattggg gaaatttcaa atcatataaa agaagctccc aatacaacgc ccagccttcg
     3301 gtccaaaaag gagtgcgaaa acggcggtac gttttatacc ggctgttcat gaaaaactga
     3361 agatgatgac ccgcgataag ttcatgatga acagtagatc ttgaaaagtg cggattatta
     3421 ccccgcatac tcatcagctt atcatcatgg tccatggtgt tggttggata ggaaatgctt
     3481 aagacttctc ctcctgtaaa aaatggattc accaattgtc ttcttggagt catcatgatc
     3541 attctccagc tttcttcggc caccggaggt attgtcagca aatcattttg tttcagaaag
     3601 tcgatggact cattgtatag tttcaggatg gcggcaggtt gctcaccttc gggaacgtac
     3661 gtgttcttta ctttttccag cgcggcatgc cagtctttgc cgaaacccaa ttcggttgaa
     3721 gcctttaaca tttcagcatc acaccaggca aactcccggt tagcaatttc aattaattct
     3781 tcaggcgtat agggaatcat ttcctgattc agctggcgga taatttcctc tctgccgatc
     3841 ggttgaccca ctattccgct gccatcgtct tgctgtgtag tgattaactt cgctttactt
     3901 ttcattaacc ggccatacct ggaaatcatg gtatcaagtt tcacaaaggg tttttccacc
     3961 caccatgtaa aatcgggatc ataaccgtta taaaattcat acaaatgctc aagacctcgt
     4021 tgaacatctt taatggcagc ctcagcccgg ttggtcaatc gtgaatcgaa gggttgggta
     4081 tcatttagtt tttgaaggcc ggcttgcagt gtttccaaag tattgttcag tactcgggca
     4141 acttcagatc cattcatgga taatccgcgg cgtcttttct tttccagctc ataaaccgga
     4201 gtcgcaaaag ttatccattc cttgatttga ttcatttcag attcctgagc ctgcagctga
     4261 tacatattgt cattcagatc tctgctgaat aaaatgtaat ctacccgcga agacacgttc
     4321 agttcatcaa aatccagggc agacaatttc ccaagatatt cctggttaag atccttaaat
     4381 ctctggatcc tttcgaaggt attctccata aaatagaacc tgctcaaact tccgtaatcg
     4441 gcgcggaact gaaccatgat attattcact tctgaagttt gcgcatagag atcagggctt
     4501 tgagaataaa gtttacttcc tgatagaatg aagaccacca ggtaaattga aagtacattt
     4561 cgaatcatga tgtatgggat taaggtgatt atttatgcaa gattttcctt tatttcttcc
     4621 tcataaaatt ttgaaccttt gggtgcccgg tacttctgaa agggtatttc aagaagattg
     4681 attaaatcat cattttcttt gggattcata tgggtatcta ttccgtacac cagtgaatca
     4741 acatcctttt ctgcaaaggt tctaaaaatc cggtcccaaa tagaaaaaat atttccataa
     4801 ttagtatctg tatagggttg cgtatagtga tgatgcacct tatgcatgtt cggggtgacg
     4861 aatatccagg ataaaatctt atcgggacct ttcggcactt tgatgtttgc gtgggttatc
     4921 tgcgcgaata tcacggacag agtttggtaa atgataatca ctcccatggg aactccggcg
     4981 atcaggacag ccagcagtgt aaaggtaatt ctgaaaatag tttctccggg atgatgtctc
     5041 aatccggtag ttacatccac gttagtatcc gtatgatgga taagatggaa cttccacatc
     5101 cactttactc tgtgctctaa ccaatggata aaataggcgc cgataaagtc gagtaataat
     5161 actcccagaa ttacatggat ccacagagga agacccagaa gataaaggat gccgaattga
     5221 ttttgagcgg tgaaatcact agccagcagc agcgcgcccg ccagtccaag acctataagc
     5281 aattgaagcg cgttcaaagt aaggttcttc agtgcatgaa caattttctt atactcaaaa
     5341 ctgaataatg gaattgttcc ctctaaaacc caaaagaatg taattccgac aatgaaaaaa
     5401 attgttctgt acactgatgg gatttccgcg aaataatcaa taatgccttc catcctattt
     5461 tacttttact gccagcgtat catgatgctc agttataaaa tttctcaggt atgcaccgat
     5521 acttccggca cttgtaaaac cggccgcttt agcagccgct tcttcctttt tatctgaaga
     5581 ctgagctgaa tttgcgtagt ctttcaaatc cttatagtac cccatggtga acatatccca
     5641 ttttgctcct tgcgttttaa caaaaatctg attcggatct cgagacaatt cttccaggta
     5701 tttattttcc atcacgcgtt catcgtaaag ttgttttcgc tgccctgaca gcgccacaaa
     5761 aatttcaaca tgaaaaaaat ccgagttatt gaatgttctg cgaaactccc tatgagtggg
     5821 tccggtgaca tataattctt ccttccagct aattaaatga tccatcggat catccaaaac
     5881 cccccggctt cctgttgtta aaagcagccc ggtgtcatcg taatcgtcaa tcgtttccat
     5941 tatcaacaaa tcccagtggt caccctggct atgcctgatt gcgtgcaaat cttttttttc
     6001 aacctccgcc tttttcttaa ttgcatcaat tagttccaat aattttccag gtgcggcccg
     6061 taccatggtt gttctataaa gttcttgcgc ggaaataaga aaaggaagca gcaaaagaat
     6121 cgtagcggag ataattcttt tcatggagga caatttaaaa aaaattacag acttaccggc
     6181 ctgattttca ctgaaaggtc tttataaacc aatataaagt tattttcagg gatcatgcgc
     6241 cagtcatcta caatatccgt caatttttct gagaccacca taacagattt ttcaccctcc
     6301 ctggccggga tcatttggca aactccttcc tggcattcat agcggctacc ttcagaataa
     6361 tacagggtca atggtttaac tgttttatcg gaggcatatc gtaatgctac cagtctttct
     6421 ccatcagtca tcaccatatt cagataagcg gggtcatcaa tcttttgctc atgcatggct
     6481 tcgagcaaaa aagcaatggt ctcttcaaaa gattgagaga tagtatcagc atctattcgg
     6541 tcctttttca ttaaattatc aagaaccaga gcgaataaat gttccgaatc ggtttggccc
     6601 tggatccaat gaaagagcgg atctgaaagt tttttatgaa cgcttctttt tattcttgaa
     6661 aaattttcaa caccgccgtt gtgtgccatc agaaaatttt tgaaatgaaa ggggtggcaa
     6721 tttgattcag aaacatctcc ttcgctggca gcccgcacgt gggcgaccag gcaatccgac
     6781 attatcttgg gtgccatgta ccgcaaattt ctgttattcc acgccggata aatgctgaca
     6841 aatacagccg ggtcctgcgc gatttctttg tgataccatc ccactccaaa gccatctcca
     6901 ttcagaggtt cttcaatttc cagggcatga aagctctgat taatcaacga gtttttgggt
     6961 tgaaaaagaa atttatccaa tacaaccggt cttcccaaat aagctaataa cctgcacata
     7021 gttttatcct aaatgttgaa aatttaagta atttccttct gatattaact gacttggttt
     7081 acatttcttt tctaactaac ccctgacata tcttgctggt cacatttgtt atcggatata
     7141 tacttgttac actcggcata ggattttggt acgcgcgcag agtcaaaagc agtgcagatt
     7201 ttgtttctgc cggtcgccaa ctgccgctaa ttctcaattc cttcgctctt tttgcgcttt
     7261 ggtacggatc ggaaaccatt tttggcgctt cggcagtttt tatggaagaa ggtcttcttg
     7321 gtgtaattga agatccattt ggagctgctc tgtgcctgat tctcttttct gttgtttttg
     7381 ccaggcgact ttaccggatg aaccttttta cgctgggcga tttattcaga aacaagtatg
     7441 gtagaaaagt tgaagtcatt gcttccttct ttatgataat aagttttttt ggatacgtgg
     7501 ccgcgcaatt agttgccctg ggattgctta tccattcctt aatgggaatt ggactggaaa
     7561 cttccattat agccagcgcg ataattgttg gcacttacac tgttgtggga ggcatgtggg
     7621 ccatatcagt tactgacttc ctgcaaagcg ttgttattat tttaggactt ggctgggtta
     7681 cctataccct tgtgagtgaa gctggagggc ttggtttggt aatagaaagt gcaccagccg
     7741 ggttctacag gttcattcct gaggcagagc cattgagttg gatagaatat ttcggagcct
     7801 ggtctgtaat tggtcttgga tcacttgcca gtcaggatgt ttttcaaaga gtaaacgccg
     7861 ctaaaagcga aaagtcagct gtgctagctg gatatcttgg aggaggcatt tatcttttgt
     7921 tttccatgtt cccactaata ataatcctaa gtattagaaa gctttatccg gaacttcagg
     7981 tttcagatac gcagcaaagt ataccggaag taattttact ccactcaagc ctgcccgttc
     8041 agattttctt atttggtgcg ttgatttccg cgattttcag tacctgcagc ggtgccattc
     8101 tggcgccggc aagtcttatg gcagaaaatg taattaaacc actttggggt aataaactta
     8161 gtgagaagag attccttata attctccgca tctctgtaat tgtgattact ttaacaggaa
     8221 tgatgatggc acttgggagc aagaacattt atgagctggt tggtgagtcc agcatacttg
     8281 gtttggtttc tatgctcatc cccatgacgg ccgctatttt ctggcagcgt gcgaacgccc
     8341 tcggtgccat cagcagtatg atcctgggat ttattttctg gctatttgcc gaacatattt
     8401 tcagtacttc aatccccgca ctattttatg gaatggtggg tgggctgatc gggctgtttg
     8461 ccggaaattt tggttcaaaa agcattaaca aaaaaataga aggtgagcac taatgatact
     8521 ataagcttca ggaaggtgcc gaaaaggaag cctgcaaaac ttcctatcgc cgaccgcatg
     8581 gcattcttcc ctttttggcc ggcatagagt tctcccagca gagctccaat caatggccca
     8641 atgattatac cgaagggtgg aaagaaaaaa atacccagca ataacccgat aatgcttccc
     8701 ataattccat atcgggaacc accaaatctt ttcgttccat agacgggaat tacataatca
     8761 aggagagtta cagctccggc aagaattccc caggtgatta gaaa
//
LOCUS       DASWHX010000198        18875 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_2080317, whole genome shotgun sequence.
ACCESSION   DASWHX010000198 DASWHX010000000
VERSION     DASWHX010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 18875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18875
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2080317"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(69..287)
                     /locus_tag="VGA21_12950"
     CDS             complement(69..287)
                     /locus_tag="VGA21_12950"
                     /inference="COORDINATES: protein motif:HMM:NF022596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HEX9651464.1"
                     /translation="MMKMGKLFIISGVILIVVGVIITLLPKNSVIGRLPGDIIIEREN
                     FKFYIPVTTGIIISIILTIILYVISRFR"
     gene            294..1016
                     /locus_tag="VGA21_12955"
     CDS             294..1016
                     /locus_tag="VGA21_12955"
                     /inference="COORDINATES: protein motif:HMM:NF012799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX9651465.1"
                     /translation="MKSLNQTKGKLLLIPNLISETKSQIELEVVSAIQNTKYFLAETP
                     KIARRFIKNIDSNIHLESLSINPYDKNTSMEELELLLQPLKQGFDVGIITDSGMPGIA
                     DPGSSAVLIAHKWGVQVKPFVGASSIFLALSASGLPGQQFSFHGYLPVSEKECTSTIK
                     KLELESSKRNSSQIFIETPYRNKRLFNLLLKTLMGDTKLCIAMDITGIEERIMTQTVS
                     KWNRQSFEFKKLPCVFIIYSGT"
     gene            1076..2326
                     /gene="metK"
                     /locus_tag="VGA21_12960"
     CDS             1076..2326
                     /gene="metK"
                     /locus_tag="VGA21_12960"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009184266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEX9651466.1"
                     /translation="MPYLFTSESVSEGHPDKIADQISDRILDNFLAMDPDSKVACETL
                     VTTGLAVISGEVKSKAYLDVQSIAREVINEIGYNRSELQFDGNSCGVISTIHEQSPDI
                     NQGVVRASEEEQGAGDQGMMFGYATNETENFMPLPLDLSHRILWELSQIRRGGKEMTY
                     LRPDAKSQVTIEYNDDNTPIRIDSIVVSTQHDEFSDEKAMRETISNDIKNILIPKVKS
                     HLPEKLQKLFTDQITYHINPTGKFVIGGPHGDTGLTGRKIIVDTYGGKGAHGGGAFSG
                     KDPSKVDRSAAYATRHIAKNLVAAGVANEVLVQVSYAIGVSKPMGIYVNTFGSSKVSK
                     KDSEIARVISEVFDMRPASIIKRLKLKEPIYSDTAAYGHMGRSSEIKKVRFRSNGAII
                     ERQVETFTWEKLDYVDKLKEAFGL"
     gene            complement(2321..2650)
                     /gene="raiA"
                     /locus_tag="VGA21_12965"
     CDS             complement(2321..2650)
                     /gene="raiA"
                     /locus_tag="VGA21_12965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018472100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEX9651467.1"
                     /translation="MKVQIHSIKFVADEKLIKYINKKLGKLEKFYSKVTDVEVMLKLS
                     NTNPENKTVEMKVNVPGEQIFVKEKSSTFEAATDIATDILKRQIKKFKDKLIKPKKSL
                     QKKGLPS"
     gene            complement(2680..3549)
                     /gene="xerA"
                     /locus_tag="VGA21_12970"
     CDS             complement(2680..3549)
                     /gene="xerA"
                     /locus_tag="VGA21_12970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009183801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase/integron
                     integrase"
                     /protein_id="HEX9651468.1"
                     /translation="MIESFLKYLKFEKRFSRHTLVSYQTDLKQFKEFLSAFDSTPEAA
                     THIDVRAWIVSLVENQISARSINRKIATLRSFYKFLLKRETITTDPSSRIKVLKTAKR
                     LPQFVKQSELNILLDQFDFKNSLNGWRDKVMLEVLYGTGIRLSELVNLEVKDIDLNKQ
                     TIKVLGKRNKERVIPFSNGLKVVILDYMKAKEGLITKNGFFLITNKGEKVYPEFVYRI
                     IKKYLKNYTSADKTSPHVLRHTFATHLLNKGADLNAVKDLLGHSSLAATQVYTHNSME
                     KLKKVFDQAHPKA"
     gene            complement(3613..3807)
                     /gene="rpsU"
                     /locus_tag="VGA21_12975"
     CDS             complement(3613..3807)
                     /gene="rpsU"
                     /locus_tag="VGA21_12975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018472098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEX9651469.1"
                     /translation="MIVINIKENESIDKALKRFKKKFEKTGVLKELRSRTYFEKPSIR
                     HRAVKLKAKYRQKMKDLENN"
     gene            complement(3895..5067)
                     /locus_tag="VGA21_12980"
     CDS             complement(3895..5067)
                     /locus_tag="VGA21_12980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009580887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX9651470.1"
                     /translation="MHTTISENPVTLSFSETENQQMIAEMIKAFGVKEITPYRKKWDD
                     DQHFPVELFHKLGKLGLMGVLVPNEYGGSGFGYHEYVTAISEIAKLDGSIGLSVAAHN
                     SLCTGHILEFGNEEQKQRWLPKLATGEWIGAWALTEPNTGSDSANMKTVAKRDGNGWT
                     LNGAKNFITHGNSGNVAVVIARTGEPGDSNGMTAFVIERGTPGLKPGRKEDKLGMRAS
                     ETSEVILEDCRVPDENVLGEVGQGFKQAMKILDGGRISIAALGLGIAQGAFEESLKYS
                     KERYQFGQPISKFQGISFKLSEMATKIEASKLLIYQAADFKNKGKKVTKESAMAKYYS
                     SEVAVEVSTEAVQIFGGYGYTKDYPVEKYYRDSKLCTIGEGTSEIQKIVISRLLLS"
     gene            5241..6569
                     /locus_tag="VGA21_12985"
     CDS             5241..6569
                     /locus_tag="VGA21_12985"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015812465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HEX9651471.1"
                     /translation="MNIAVIGTGYVGLVTGTCLAETGNKVICVDIDEEKVNSLQSSKI
                     TIYEPGLEPIFERNIRQGRLQFTTSLETGVKSSDIIFLALPTPQGENGSADLSHILEV
                     AKKIGPLLDKYKVIVDKSTVPVGTSDKVRKMISESTTSEFDVVSNPEFLREGVAVDDF
                     MKPDRVVIGTNSERARSLMEKLFEPMVRQGNPILFMDERSAELTKYAANSFLATKITF
                     MNEIANLCELMGADVDMVRKGIGTDTRIGNRFLFAGIGYGGSCFPKDVKALGESSREL
                     NYDFRILDAVMQVNEKQKTKLIDKAKKYFNGSLFNGSLKGKHFAVWGLSFKPYTDDIR
                     EAPSLSNIKELLNLGATISAFDPEGMDNVKKVLGDSIRYSVNEYECLENADGLFIFTE
                     WPVFRTPDFDILKNKMKGHVIFDGRNLYSIKSIMNLGFNYFSIGRKDVVQ"
     gene            6566..7558
                     /locus_tag="VGA21_12990"
     CDS             6566..7558
                     /locus_tag="VGA21_12990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009580889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronic acid decarboxylase family
                     protein"
                     /protein_id="HEX9651472.1"
                     /translation="MSKLKVLVTGGAGFLGSHLCEKFLQEGYQVFAMDNLITGDLRNI
                     NHLLSNHDFEFINHDVTKFVDFPGNLNFIMHFASPASPVDYLKIPIQTLKVGALGTHN
                     LLGLAKAKNARMLVASTSEVYGDPLVHPQNEEYYGNVNPVGPRGVYDEAKRFLEAITM
                     AYHRFHKLDTRIVRIFNTYGPRMRLNDGRVLPAFIGQALRSEDLTVFGDGSQTRSFCY
                     VDDLVDGIYKLLLSSYSMPVNIGNPDEITILDFAKEIIKLTGTRQKIVFKPLPANDPK
                     QRQPDITKAKKLLSWEPKIHRSEGLKITYDYFKSLPMEVLKKIEHHTFENYIKP"
     gene            7555..8580
                     /gene="galE"
                     /locus_tag="VGA21_12995"
     CDS             7555..8580
                     /gene="galE"
                     /locus_tag="VGA21_12995"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010610811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEX9651473.1"
                     /translation="MSGKILVTGGAGFIGSHTVVELSNSGYEPVILDDFRNSEKSVLL
                     GLKNILKRDVKCYNANCCEADTVEKIFDKEQFNGVIHFAAFKSVNESLKIPVQYYYNN
                     IVSLLVLIEAMRKFNVHEFVFSSSCTVYGQPEELPVTESSPFKEAFSPYGSTKQISEK
                     ILIDAFKSNLPLRTMSLRYFNPIGAHPSGEIGELPIGYPNNLVPYLTQSAAGVRGPLT
                     VFGNDYNTPDGTCIRDYIHVVDLAKAHVKSLGYLAGVKEESTFEAFNVGTGNGTSVLE
                     LIHSFENANVIKLNYDIGPRRPGDAESIYANCDKAKDQLGWETSLTLEDALRDAWNWQ
                     KRLSNNK"
     gene            8577..9626
                     /gene="rfbB"
                     /locus_tag="VGA21_13000"
     CDS             8577..9626
                     /gene="rfbB"
                     /locus_tag="VGA21_13000"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017729998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEX9651474.1"
                     /translation="MKSEKTILITGGAGFIGSHVVRLFVNKYPNYRIINLDALTYAGN
                     LENLRDIESKPNYVFVKGNVCDKISVAATMSASNITDIIHLAAESHVDRSITDPLQFV
                     HTNILGTLTLLECCRDLWKNTDSRFYHISTDEVYGSIDEGLFTEYTPYNPNSPYSASK
                     ASSDHFVRAYGNTYGLPVVISNCSNNYGSYQFPEKLIPLIINNILERRPLPVYGKGEN
                     VRDWLFVEDHAKAIDLIFHQGRNGETYNIGGQSEYKNLDLVHLICEEADKIINPDTSS
                     IELITFVKDRPGHDLRYAIDISKLKNELGWSPSVSLKVGLNKTILWYIENQEWLRHVT
                     SGAYKKYYNLNYSNR"
     gene            9626..10510
                     /gene="rfbA"
                     /locus_tag="VGA21_13005"
     CDS             9626..10510
                     /gene="rfbA"
                     /locus_tag="VGA21_13005"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018479680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEX9651475.1"
                     /translation="MKGIILAGGTGTRLHPITLGISKQMIPVYDKPMIYYPLSVLMLA
                     SIKKILIISTSHDLPLFKQLLGDGSQWGLKLEYAVQEKPEGLAQAFLIGERFIGKDKV
                     ALILGDNIFYGRGMGTLLRNYKNPRGGVVFAYRVNDPERYGVVEFDAKMKAVSIEEKP
                     KKPKSNYAVPGLYFYDNDVVSISKKLKPSTRGELEITDVNNMYLKKGKLKVGVLGRGT
                     AWLDTGTFDSMVQASNFVQIVEKRQGLKICCPEEIAYRMGFIDKKQLIKLAKPLEKSG
                     YGAYLLKILEDENPIRNK"
     gene            complement(10494..11474)
                     /locus_tag="VGA21_13010"
     CDS             complement(10494..11474)
                     /locus_tag="VGA21_13010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018479607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEX9651476.1"
                     /translation="MKNSILIVTIILFSCGEKKSQEIISRPAIKNVSVPPFNSDSAFH
                     FLKKQVDFGPRVPNSREHELTGNYLISKLAEYGGTITVQNFTATSADGVKLNLRNIIG
                     SFHEGKTRRILLAAHWDTRPYADKDSADMDKPIDGANDGASGVAVLLEIARVISINTP
                     PDAGIDIILFDGEDWGENMLRPSTPLESGLETWYCLGSQYWAKNKHKRGYSAYFGILL
                     DMVGAKDSKFFIEGLSYEMAPKIVEKVWQTASQLGYSNYFVNQRKGAITDDHEFMNAA
                     GIPSINIVHYDPQYGYFGDYHHTHRDNLDLIDKNILEVVGSTVMHVIYYE"
     gene            complement(11464..12951)
                     /gene="cysS"
                     /locus_tag="VGA21_13015"
     CDS             complement(11464..12951)
                     /gene="cysS"
                     /locus_tag="VGA21_13015"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013454567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEX9651477.1"
                     /translation="MPENLVVFNTLTGKKEEFKPINPPFVGMYVCGPTVYSDVHLGNV
                     RTFISFDVIYRFLTYKGYKVRYVRNITDVGHLESDADEGEDKISKKAKLENLEPMEIV
                     QRYATGFHHVMDLFNILPPRIEPSATGHLIEQIEMIKKLIENGFAYEANGSVYFDIKK
                     YNESHQYGILSKRVLEDLMAGTRALDGQDEKRFPLDFALWKKAAPGHLMKWDSPWGLG
                     FPGWHLECSVMGTKYLGETFDIHGGGMDLKFPHHECEIAQSIGAYGKAPVNYWLHTNM
                     LTVNGQKMSKSLGNSFLPEQLVKGDHELLDQAYSPMTVRFFMLQSHYSSTLDFSNEAL
                     KAAARGYKRLMNGIRISRKLKFERVDGKVNKDLVKEINDSIQGCKDAMNDDFNTALTI
                     SHIFNLLKKVNSLHTGQIGFDEISEPVFEKMCTTFIDFTEKVLGLREENPARPVEVIE
                     ALLKVYKQAKFKKEYDKVDMIRADLKQSGIILKDMKTGVEWAYEE"
     gene            13112..13588
                     /locus_tag="VGA21_13020"
     CDS             13112..13588
                     /locus_tag="VGA21_13020"
                     /inference="COORDINATES: protein motif:HMM:NF014617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor"
                     /protein_id="HEX9651478.1"
                     /translation="MKLEENSLKDLVEKNLEYESLFLVAVKVSRHGDSQRVKIILDGD
                     NGITIEQCAEVSRKISQVLDEDEGDQSPFVLEVTSPGVDHPIRMFRQYVKNVGRRVKV
                     ILQDRIEIKGILKSVNEKFIEVEKEIKSKNKKEHKSEMVEIPFDKIEKTNILISFK"
     gene            13597..14838
                     /gene="nusA"
                     /locus_tag="VGA21_13025"
     CDS             13597..14838
                     /gene="nusA"
                     /locus_tag="VGA21_13025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009280761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HEX9651479.1"
                     /translation="MDSLNLIETFAEFAKGKNIDRPTMIRILEDVFRTMIRKKYDQDD
                     NFDIIINVDKGDLEIWRFREIVDDNSEDIWDYDKITLTDARKIEADFEIGEEVAEDIK
                     LEDFGRRAVMTARQTLMQKIKDLEKDILFHKYKDLVGEIINGEVYQILSREVLLIDGE
                     GNELTIPKSEQIQKDRYRKGDTARAVVHRVEMVNGNPKIILSRTSPVFLERLFESEVP
                     EVYDGLITLKKVVREPGERAKVAVESYDDRIDPVGACVGMKGSRIHSIVRELQNENID
                     VINFTENLELYIQRALSPAKISSIKINAEEKRVSVYLKPDQVSLAIGKGGQNIKLASR
                     LVDMEIDVFREIGDYEEEDVDLEEFGDEIENWIIDEFKKIGLDTAKSVLALSMEDLIR
                     RTDLEEETIAEVVRILKKEFE"
     gene            14880..17513
                     /gene="infB"
                     /locus_tag="VGA21_13030"
     CDS             14880..17513
                     /gene="infB"
                     /locus_tag="VGA21_13030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011586765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEX9651480.1"
                     /translation="MAEQGTMRLGQVARKLNVGIHTITEHLASKGFEVEDKPNTKITP
                     EQFNILAKEFAASLLEKEEASGLTIGAKHNENVVIEKSRTEQRESEDDDEILIKNLSL
                     EKDGEVTDVGKETEESEKPKLKGITVVGKIELGAKKEEAKQESEEKETITKETSAGTR
                     ESGTEPVPESELIKAKSDKLQGLKVVGKINLPARKEKEKEKSEPFASSDKSADRKKRP
                     RKRIPTQKPPQRTGDRTRRRPEKEEPSEKEIQEQIKATLAKLSGGKKTSAGAKYRREK
                     RISKAEAHEEKVLQEMRDSKVLKVTEFISASDLASLMNVSINDVISTCLNLGMFVSIN
                     QRLDAEAITIIADEFKYSVNFTTSEDETEIYEEEDSEEDLKPRAPIVTIMGHVDHGKT
                     SLLDYIRKSKVTAGEAGGITQHIGAYDVLTEGGQKIAFLDTPGHEAFTAMRARGAKVT
                     DIVIIVVAADDNVMPQTKEAINHAQVAGVPIVIAINKIDKPNANPEKIKEELSKVNIL
                     VEDWGGKYQCQAISAKLGQGVNELLEKVLLEAELLELKANHDKPAIGTVIEASLDKGR
                     GYVTTLLVQNGKLKISDIILAGPYYGRVKAMFDHTGTKMNEVGPSTPVLVLGLDGAAQ
                     AGDKFNVMESEREAREISVKREQILREQNIRTKKHITLDEIGRRLAIGSFRELNVIVK
                     GDVDGSVEALSDSLLKLSTGEVQINIIHKGVGQISESDVLLATASDAVIVGFQVRPSA
                     SARRLAESEEIEIRLYSIIYDAINEIKDAMEGMLAPEVEEVITGNAEVRSVFKITKVG
                     TVAGCMVTEGVIKRNSEIRLVRDGIVIYEGQILQLKRFKDDASDVKSGFECGISIKNF
                     NDIKVGDVIESYEKKEVKRTL"
     gene            17523..17714
                     /locus_tag="VGA21_13035"
     CDS             17523..17714
                     /locus_tag="VGA21_13035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651481.1"
                     /translation="MEFVQKFLLTLIGLSLFFLVLGLYKPYWMVWWEDIQNRKKVLSV
                     YLTSAIFFLCIYLVISYLY"
     gene            18060..>18875
                     /locus_tag="VGA21_13040"
     CDS             18060..>18875
                     /locus_tag="VGA21_13040"
                     /inference="COORDINATES: protein motif:HMM:NF037531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651482.1"
                     /translation="MKFSTENASYSLENENFPWQQNLNGMIEEFEYKGRWRVPDEEFW
                     YNGALKFHPGEPIILEIFGTFNPGMFDTEKKGIILGETTKGDITLVDSDYITHSHSFV
                     SDVTIGKYRPSYIIAGHQFYSAESIQFSKTTFKTFNLFTWLDTAGLIRDERNLLPSEY
                     TKPESLSFKCFEECSGAIDFHHTLNLIFRNNRTEVEEDCSVSLSYNKSRHFKNILMDI
                     YIFNGFITLMTFEQCYPLSISFTDPSYTEDNKAKIIQCFYQNPDYNKKYKARSK"
BASE COUNT         5883 a         3595 c         3873 g         5524 t
ORIGIN      
        1 gccgggcact gggtacacgc ggaacaaccc gagttattta ttaccctctt aaatgacttc
       61 ttgaagtctt agcgaaaccg gcttattacg taaaggatta ttgtaagaat gatactgata
      121 attatgccgg tggtgacggg gatataaaac ttgaaatttt ctctttcaat tattatatcc
      181 cccggcaacc ttccgataac tgaatttttg ggtaaaagtg taatgataac cccaacgaca
      241 attaagataa ctcctgatat tatgaataat tttcccatct tcatcataat agaatgaaat
      301 ctctaaatca aacaaaagga aagcttttat taattccaaa tttgatttcg gagacaaaat
      361 cacaaattga gcttgaggtt gtttccgcta ttcagaatac aaaatacttc cttgccgaaa
      421 cgcccaaaat agcgaggaga ttcataaaaa acatagactc aaacattcat ctggaatcac
      481 ttagtataaa tccatacgat aaaaatacct cgatggaaga attggaattg ttacttcaac
      541 ctttaaaaca aggctttgat gttggcatta ttacagattc aggaatgccg ggtatagctg
      601 atcccggaag ttcagcggtc ttaatagctc ataagtgggg tgtgcaagtg aagcctttcg
      661 ttggtgcttc ctctattttt cttgctttat cagcttcagg gttgccaggc cagcaattct
      721 ctttccacgg ctacttaccg gtgtccgaaa aagaatgcac atcaacaatt aaaaagcttg
      781 agttggagtc atctaaaaga aattcaagcc agatttttat agaaacacca taccgaaata
      841 aaagactttt taatcttttg ttgaaaaccc ttatgggtga taccaaattg tgcatcgcta
      901 tggatattac cggaattgaa gaaaggataa tgacccaaac ggtatcaaaa tggaatcggc
      961 aatcctttga attcaaaaaa cttccctgtg ttttcataat ttattccggt acctgaaatt
     1021 tctttgttgg gctattatac ctaaatttgc gttccatttt atttaaatat tgaacatgcc
     1081 ctatttattc acatctgaat ccgtttcaga aggtcatcct gacaagattg ctgatcaaat
     1141 ttccgatcgc attcttgata attttctcgc tatggatccg gattcaaagg ttgcctgtga
     1201 aacactggtt actaccggtc ttgcggtaat tagtggtgaa gtgaaatcca aagcttacct
     1261 ggatgttcaa agcattgccc gggaggttat taacgaaatt ggttacaata gatctgaact
     1321 tcagtttgat ggaaattctt gcggagtaat ctctaccatt catgagcaat caccggatat
     1381 taaccagggt gtcgtccggg cttcggaaga ggaacaaggt gccggcgacc aggggatgat
     1441 gtttggatac gcaactaatg aaactgaaaa ctttatgccg ctgcccctcg acttgtcaca
     1501 tagaatcctg tgggagttgt cgcaaatccg tagaggcgga aaagaaatga cttacctgag
     1561 gcccgatgcg aaaagtcagg taacaattga atataacgat gacaatacgc ctataagaat
     1621 tgattcaatt gtcgtttcca cccaacatga tgaattcagc gatgaaaaag ccatgcggga
     1681 aacaattagc aacgacataa aaaacatttt gattccgaag gttaaatcac atctcccgga
     1741 gaagcttcaa aagcttttca ccgaccaaat cacctatcat ataaatccaa ctggtaaatt
     1801 tgtaattgga ggccctcatg gtgacaccgg cctgaccgga agaaaaatta ttgtagatac
     1861 gtatggtgga aaaggtgcgc atggcggtgg tgctttttca ggaaaagatc cctccaaggt
     1921 agaccggtcg gcagcctacg ccacccggca tattgcaaag aatcttgttg ctgcaggagt
     1981 tgccaatgaa gttttagtcc aggtatctta tgctatagga gtttccaagc ccatggggat
     2041 ttatgtgaat acttttggaa gctctaaagt gagcaaaaag gatagcgaaa tagctcgcgt
     2101 tatttcagag gtttttgaca tgaggcctgc ttcaataatc aaaagactta aactgaaaga
     2161 acccatttac tctgatactg ccgcttacgg gcacatgggc agaagttctg aaatcaagaa
     2221 ggttcggttc agatcaaatg gcgctattat tgaaagacaa gttgaaacct tcacctggga
     2281 aaagcttgat tacgttgata aattaaaaga agcttttgga ttatgaagga agccctttct
     2341 tttgaagact ctttttaggc ttaataagct tgtccttaaa tttttttatt tgacgtttaa
     2401 gaatatccgt tgcaatatca gtcgctgctt caaaagtgct tgacttttcc tttacaaaaa
     2461 tttgttcgcc cggaacatta actttcattt ccaccgtttt gttttcagga tttgtattac
     2521 tcaacttcag cattacttcc acatcagtaa ccttgctgta gaatttctcc aatttcccca
     2581 gcttcttgtt gatatattta atcaacttct catcggcaac gaattttatc gaatgaatct
     2641 gcactttcat ataattagag tttaattttt cacatttaac taggcttttg gatgcgcctg
     2701 atcaaaaacc tttttaagtt tctccattga attgtgtgta taaacctgtg tagcagccag
     2761 actggaatgc ccgagcaaat ctttgacagc atttaaatct gcgcctttat ttaacaaatg
     2821 tgttgcaaac gtgtgcctca aaacatgggg actggtctta tctgcactcg tataattctt
     2881 tagatatttc tttattattc tgtaaacaaa ctccgggtat accttttcac ctttatttgt
     2941 aattaagaaa aaaccattct ttgtaattaa cccctccttt gctttcatgt agtcaagaat
     3001 tacaactttt aatccatttg aaaatggtat tacgcgctct ttgtttcttt ttcctaaaac
     3061 tttaatggtt tgcttattaa ggtcgatatc tttcacttcc agattaacca actctgaaag
     3121 cctgattccg gtaccatata ggacttcaag cattactttg tctctccagc catttaaaga
     3181 atttttgaaa tcaaactgat ccagcagaat attcaattcg gattgcttaa caaattgagg
     3241 taatcttttt gctgttttca gtaccttaat tcttgatgat gggtctgttg taatggtttc
     3301 tctttttaat agaaatttat aaaaggaccg cagagttgca attttccggt taattgaccg
     3361 cgcacttatc tgattctcta ccaggcttac aatccaggca cgaacatcaa tatgagttgc
     3421 tgcttctgga gtggaatcaa aagctgaaag aaattctttg aactgcttta aatcggtttg
     3481 ataagaaacc agtgtatgcc tgctgaaacg cttttcaaat tttaggtatt tcaaaaagga
     3541 ctcaatcatc atctttacgt tcccaccgca gtaacactaa cttaacaaaa aaaatgataa
     3601 ttgggaagga cgttagttat tttccaggtc cttcattttt tggcgatatt tagcttttag
     3661 tttcacagct ctatgcctta tggagggttt ctcaaagtac gtcctcgacc ttaattcttt
     3721 caacacgccg gttttctcga atttcttctt aaaacgctta agtgccttat caatagactc
     3781 gttttctttt atgttaatta caatcataat attctattcg ggttgcaaag ttattaaaag
     3841 aatagcaaat aggccaccct gtgcctgaaa aaataaaagg aatgcgggtg taaattatga
     3901 aagtaataac cttgaaatga ctattttttg gatttcagag gttccttcac caatggtaca
     3961 tagcttggag tctctataat atttttcaac ggggtagtcc tttgtgtatc catatcctcc
     4021 aaatatctgc accgcttctg tcgatacttc aacggcaact tctgaagaat aatattttgc
     4081 cattgcactt tccttggtta ctttcttgcc cttgttctta aagtctgctg cctgataaat
     4141 gagaagttta gatgcttcta ttttagtcgc catttctgac aatttgaatg aaatcccctg
     4201 gaatttagaa atcggctgtc cgaactgata tctttccttt gaatacttta atgattcttc
     4261 aaaagcaccc tgggcaattc ccagaccaag cgctgctatg ctgattctac ccccatcaag
     4321 aattttcatt gcctgcttaa acccctggcc aacttcgccc agtacattct catcaggtac
     4381 cctgcaatct tctaaaatta cttcacttgt ttcagaggct cgcattccca atttgtcttc
     4441 ctttctgcca ggcttaagac cgggggttcc tctttcaatt acaaatgcag tcattccgtt
     4501 ggaatcaccg ggttccccgg ttcttgctat aactacagcc acgtttccgg aatttccatg
     4561 ggtaataaag ttcttggctc catttaatgt ccacccattc ccatctcgct ttgctacggt
     4621 tttcatatta gccgagtcgg agcctgtgtt tggctctgtt aaagcccagg caccaatcca
     4681 ttcaccggtg gcaagtttgg gcagccatct ttgtttttgt tcttcattcc caaattccag
     4741 tatatgtcct gtgcataagc tgttgtgtgc agctactgat aaacctatgc tcccgtccag
     4801 ttttgctatt tccgatattg cagtcacata ttcatgatac ccgaaaccag aacccccata
     4861 ctcattaggg accaatacgc ccatcaaacc aagttttccc agtttatgaa acagttcgac
     4921 aggaaagtgt tggtcatcat cccatttctt ccggtagggg gtaatttctt tcactccaaa
     4981 agcttttatc atttcagcta tcatttgctg attttctgtt tcactgaaag ataatgtaac
     5041 cggattttcg ctaattgttg tatgcataaa ctattgacct ttagatattt aagtagtttt
     5101 gatataaaat gttcgctaaa ataacaactt tttaagcttg tttacaaaat cttttgaggg
     5161 attgaatgaa taaattgatt taaaatccct attaagtaat aacacaggct aactttacgt
     5221 ccaaaaacaa gtttcaaggg atgaatattg ctgttattgg cactgggtac gtaggattgg
     5281 ttaccggaac ctgtcttgct gaaaccggga acaaagttat atgcgttgat attgatgaag
     5341 agaaggttaa ttctttgcag agtagtaaaa taacaattta tgaacctgga cttgagccca
     5401 tttttgaaag aaatatccga cagggaagac ttcaattcac aacaagtctg gaaacaggag
     5461 ttaagagctc tgatattatt tttcttgctt tgccaacacc ccaaggtgaa aatgggtcag
     5521 ccgacttatc ccatatactt gaagttgcaa aaaaaatcgg acccctgtta gacaagtaca
     5581 aagtgattgt tgacaaaagc actgtgcctg ttggaacttc ggacaaagtg aggaaaatga
     5641 tttcagaaag tacgacatca gaatttgatg tagtatccaa cccggaattc ctgcgtgaag
     5701 gtgtggctgt tgatgatttt atgaagccag acagggtagt aatcgggaca aactcggaaa
     5761 gagcccgatc tcttatggaa aaattatttg agccaatggt tcgtcagggg aatcccatcc
     5821 ttttcatgga tgaaaggtcg gctgagctaa ccaagtatgc agccaattct tttcttgcaa
     5881 ccaagattac cttcatgaat gaaattgcta acctctgcga attaatggga gctgatgtag
     5941 atatggtgag gaaaggtata ggtaccgata cccggatagg aaaccggttc ttatttgcgg
     6001 gaataggata tggaggaagc tgctttccaa aggacgtgaa agctcttggt gaatcatcca
     6061 gggagctgaa ttacgatttc agaattttgg atgcagtaat gcaggtaaat gaaaagcaaa
     6121 aaacaaaact tatagacaaa gcaaaaaagt atttcaatgg gtcgttattc aatgggtcgt
     6181 taaagggaaa gcattttgct gtttggggtt tgtctttcaa accttatacc gatgatattc
     6241 gtgaagcacc aagtttatca aatattaaag agttgttgaa tttaggcgca acaataagcg
     6301 cgtttgatcc tgaaggaatg gacaatgtta agaaagttct gggtgattca attcgttatt
     6361 ctgttaacga atacgagtgt cttgaaaatg cagatgggct tttcattttc actgaatggc
     6421 ccgtgttccg aacccccgat ttcgatattt taaaaaataa aatgaaaggg catgttattt
     6481 tcgatggcag gaatttatac tcaattaaat caattatgaa ccttggtttc aattatttca
     6541 gtattggaag aaaggacgtt gttcaatgag caaacttaag gtgttagtta caggtggtgc
     6601 gggctttctg ggatcgcacc tttgtgaaaa atttctgcag gaaggttatc aggtattcgc
     6661 gatggataat ctcattaccg gcgacttacg aaacattaat cacctccttt ctaaccatga
     6721 ttttgaattt atcaatcatg acgtcacaaa gtttgttgat ttccccggca acttaaactt
     6781 tataatgcac tttgcctcac ccgccagtcc tgttgattac ctgaaaatac ccattcaaac
     6841 attaaaagtt ggtgccctag gaacacacaa cttgcttggc ctggctaaag ccaaaaatgc
     6901 caggatgctg gttgcctcta cttccgaagt ttacggtgac ccattggtgc accctcaaaa
     6961 tgaagaatac tacggaaatg taaaccccgt gggaccaaga ggggtttatg atgaggctaa
     7021 acgatttctg gaagccataa caatggctta tcaccgcttc cataaattgg ataccaggat
     7081 cgtcagaatt ttcaatacct atggtccgag gatgcgcctg aatgatggaa gggttctgcc
     7141 tgcatttatt ggacaagctt tgcggagtga agatttgaca gtttttggtg atggaagcca
     7201 gacaagatca ttctgctacg tggatgacct ggttgatgga atttataaat tattattaag
     7261 cagttattcc atgccggtta atattggaaa cccggatgaa attactattc tcgatttcgc
     7321 caaagaaatc ataaaactca ccggtaccag gcagaagatc gtgtttaaac ctcttcctgc
     7381 caatgatccc aaacagcgcc agccggacat tactaaggct aaaaaacttt tgagttggga
     7441 accaaaaata catcgcagcg aaggacttaa aataacttac gattatttca aatcacttcc
     7501 tatggaagta ttgaagaaga tagaacatca tacttttgag aattatatta aaccatgagt
     7561 ggtaagattt tagtaacagg cggagcaggg tttattggtt ctcacaccgt ggttgaattg
     7621 tcaaactccg gctatgagcc tgtaatcctg gatgacttca ggaattcgga gaaaagcgta
     7681 ctgctcggat taaagaacat cctgaaaagg gatgtgaaat gctataatgc aaattgttgt
     7741 gaggccgata cggtggagaa aatttttgat aaagagcaat ttaacggtgt catacatttc
     7801 gcggcattca aatccgttaa tgaatcgctg aagattccgg ttcagtatta ttataataac
     7861 attgtttcct tgcttgtgct aattgaggca atgaggaagt ttaatgtgca cgagttcgta
     7921 ttttcctcct cctgcactgt atatggccag ccggaagaat tacctgtgac cgaaagttct
     7981 ccgtttaaag aggctttttc tccatatggt tcaacaaaac aaatttcaga gaagatactg
     8041 atagatgcct ttaagtctaa ccttccgctc aggacaatgt ccctcaggta ttttaatccc
     8101 attggagctc atccttccgg tgagattggt gaattaccaa ttgggtatcc aaataacctt
     8161 gttccatatc ttacacaaag cgcggctgga gtccggggac cacttaccgt atttggaaat
     8221 gattataata cgcctgatgg aacctgcata agagattata ttcatgtggt ggaccttgcc
     8281 aaggcacatg tgaaatcact gggatatctg gccggtgtca aagaagaatc cacttttgaa
     8341 gcttttaatg taggaaccgg aaacggaacc tcagttctgg aattaattca tagttttgaa
     8401 aatgccaatg tgattaagtt gaactacgac attggtcccc gaagacctgg cgatgcggag
     8461 tccatttatg caaattgcga taaggccaaa gatcaactgg gttgggaaac ttctttaact
     8521 ttggaagatg cactgcggga tgcctggaac tggcagaaac gcttaagtaa caacaaatga
     8581 aatctgaaaa aacaattctg ataacgggtg gtgccggctt tattggaagc catgttgtaa
     8641 gactttttgt gaataagtat cccaactatc gaattatcaa tttagatgca ttgacctacg
     8701 ccggaaacct ggagaatctc cgggatattg aatcaaagcc aaactatgtt tttgtgaagg
     8761 gaaacgtttg cgacaaaatt tcggtagccg caacaatgag cgcaagcaat ataaccgaca
     8821 taattcatct tgccgcggag tctcatgttg acagatccat aacagatccc ctgcagtttg
     8881 ttcacacaaa tattttaggc acacttacct tgcttgagtg ttgtcgtgat ctttggaaaa
     8941 acacagacag ccggttttat catatcagca ctgacgaggt ttatggatcc attgacgaag
     9001 gtctttttac tgaatatact ccctataatc caaactcacc ttattcagca tcgaaagcaa
     9061 gttctgacca ttttgttcgg gcttacggaa atacttatgg cctccctgta gttatttcaa
     9121 attgcagcaa taactatggt tcatatcaat ttccggaaaa attaatccct cttataatta
     9181 ataatatttt agagagaaga cctttgcctg tttatggtaa aggagaaaac gttcgcgatt
     9241 ggctttttgt tgaagatcat gcgaaagcta ttgaccttat ctttcaccag ggaagaaatg
     9301 gggaaaccta taacattgga ggccaaagtg aatataagaa tcttgatctt gtacatctga
     9361 tttgcgagga agctgacaag atcattaacc ctgacacatc ctcaatagaa ttaataacct
     9421 ttgtgaaaga cagacccggc catgatttgc gatatgcaat tgatatttca aaattaaaaa
     9481 atgaattggg ctggtcgccc tcggtttcat tgaaggtagg tttaaacaaa acaattttat
     9541 ggtatattga aaaccaggaa tggttaagac atgttacatc cggagcttat aagaaatact
     9601 acaatttaaa ttatagcaac agataatgaa aggtattatt ctggcaggtg gaactggtac
     9661 caggctgcac ccaataacgt tgggtattag caagcaaatg attcctgtct acgataaacc
     9721 catgatctat tatcctctgt ctgttttgat gctcgccagc ataaagaaga ttctaatcat
     9781 ttctacatcg cacgacttac cgctctttaa acaattgctt ggagatggtt cccaatgggg
     9841 attaaaactt gaatatgctg tacaggagaa gccggaggga ttggctcaag ccttcctaat
     9901 cggtgaaaga tttattggaa aggataaggt cgcccttatt cttggtgata atatttttta
     9961 cggtcgcggt atgggaactt tgttgcgaaa ttataaaaat cccaggggcg gtgtagtatt
    10021 tgcctaccgg gtcaatgacc cggagcgata tggcgttgta gagtttgatg caaaaatgaa
    10081 ggctgtgtct attgaagaga aaccaaagaa gccaaaatcg aattacgctg tgcccgggtt
    10141 atatttttat gataatgatg ttgtttcaat ttcaaaaaaa ttaaaaccaa gtaccagggg
    10201 tgaacttgaa attaccgatg taaacaatat gtatcttaaa aaaggtaaac tgaaagtagg
    10261 agtgctggga agaggaactg catggcttga taccggcacc tttgattcaa tggtacaggc
    10321 ttctaatttt gttcaaatcg tagaaaaaag gcaaggattg aaaatttgtt gtccggaaga
    10381 aattgcttac aggatgggct ttatagataa aaaacagcta atcaaactgg cgaagcccct
    10441 ggagaaaagt gggtatggtg catacctgtt gaaaatcctg gaagatgaga atcctattcg
    10501 taataaataa catgcatcac cgtactgccg accacctcta aaatattttt atcaatcaag
    10561 tcaagattat cgcggtgggt gtgatggtag tctccaaagt acccatattg aggatcataa
    10621 tgcacaatat taatggaggg aattccggct gcattcataa attcatgatc atctgtgatg
    10681 gctcccttcc tttgattaac aaagtaatta ctatatccta attggctcgc ggtttgccaa
    10741 actttttcca cgatttttgg tgccatttca tagcttagcc cctcaataaa aaattttgaa
    10801 tctttagcac caaccatatc cagtaaaatt ccaaagtatg ccgagtatcc cctcttatgc
    10861 ttattctttg cccaatattg cgaacccagg cagtaccagg tttccaatcc gctttcgaga
    10921 ggcgttgatg gccttagcat attttctccc caatcctccc cgtcaaacag aataatatca
    10981 atgcccgcat caggtggagt atttatactt ataacccggg ctatttcaag gagaacagct
    11041 acaccactag ctccatcatt ggctccatca attggtttat ccatgtctgc tgaatcttta
    11101 tcagcatagg ggcgggtgtc ccagtgtgcg gccagtaaaa ttctccgggt ttttccctca
    11161 tggaaggaac ctataatgtt gcgcaaattt aatttaactc catccgcgga ggttgcagta
    11221 aaattttgca cagtaatagt accaccatat tcagctaact tagagattag ataattacct
    11281 gttaattcat gctcccggga attaggaacc ctggggccaa aatcaacctg ttttttcaga
    11341 aagtgaaaag cagaatcgct attgaatgga ggtacagata catttttgat ggccggtctg
    11401 cttatgattt cctgcgattt tttttctccg cagctgaaca gaataatggt tacaatcagg
    11461 atgctattct tcataggccc actcaactcc agtcttcata tctttcaaaa taatgccaga
    11521 ttgctttaag tcggctcgaa tcatgtctac tttatcgtat tctttcttaa acttggcctg
    11581 cttatacact tttagcaacg cttcaatgac ctcaaccggc cgggctggat tttcttctcg
    11641 taatcctaac actttctcag tgaaatcaat aaatgtagtg cacatctttt caaacaccgg
    11701 ctcactgatt tcatcaaaac ctatttgacc ggtatgaagt gaatttacct tcttcagcaa
    11761 attaaaaata tgagaaatag ttaacgcggt attaaagtca tcattcatgg catctttaca
    11821 gccttgaatg gaatcgttaa tctctttcac caaatcttta tttacttttc catcgactct
    11881 ctcaaatttc aatttcctgg aaatccggat tccattcatc agtcttttat atccccgggc
    11941 tgcagctttc agggcttcat tactaaaatc caatgtgctg gaatagtgtg attgcaacat
    12001 gaagaaacga accgtcatag gactgtaagc ctgatccaat aattcatgat cacccttcac
    12061 gagttgctca ggaagaaatg aattgcccag cgacttgctc attttctgtc cattcacagt
    12121 aagcatgttt gtgtgcagcc agtaattaac gggagccttc ccataggctc caattgattg
    12181 agctatttca cattcgtgat gaggaaattt caaatccatc ccccctccat gaatatcgaa
    12241 agtttctccc aaatatttag tgcccattac ggaacattca agatgccaac cgggaaaacc
    12301 caaaccccat ggtgagtccc atttcattaa atggcctgga gccgcctttt tccataaagc
    12361 aaagtcaagc ggaaatcttt tttcatcctg accatcaagc gccctggtcc cggccatcaa
    12421 atcttccagc acgcgttttg acaaaatccc atactggtgg gactcattat attttttgat
    12481 atcaaaataa actgagccat tcgcttcata ggcaaatccg ttttcaatta gcttttttat
    12541 catctcgatc tgctcaatta aatgcccggt agcgctgggt tcaatacggg ggggaagaat
    12601 attgaataga tccattacgt ggtgaaatcc ggttgcataa cgctgcacaa tttccatggg
    12661 ttccaggttt tccagtttcg ccttttttga aatcttatct tcgccctcgt cagcgtcact
    12721 ttccaggtgt cccacatctg tgatatttcg cacatatctt actttatacc ctttgtaggt
    12781 aagaaaccgg tatataacat cgaaactgat aaaagtccga acattgccca agtgcacgtc
    12841 actataaacc gttggtccgc atacatacat tccaacaaat ggcgggttaa tgggtttaaa
    12901 ctcttctttt ttgcccgtca gggtattgaa aacaacaaga ttctctggca tggatgcaaa
    12961 aataaccaaa taaattattt aggaatcagc tattttgaag attggcgtca aagttttgct
    13021 ttaacgcctg atttcgatta tttttgcctt gaaactgata gtaaacaatt ttctgagggg
    13081 gctaaatggt cccctctttt tatgcatcat aatgaagctt gaggaaaata gtctgaagga
    13141 tttggttgaa aagaacctgg aatatgaaag cttgttcctg gttgcagtca aggtttcccg
    13201 gcacggagat tcacagcgag tgaaaattat cctggatggg gacaatggaa ttaccataga
    13261 acaatgcgct gaagttagca gaaaaatcag tcaggtactg gatgaggatg agggggatca
    13321 aagcccattt gtactggaag ttacaagtcc cggagttgac caccccattc gaatgttcag
    13381 acaatatgta aagaatgtgg gaaggagggt taaggtgatt ctccaggacc gaatcgaaat
    13441 taaagggatt ttaaaatcag taaatgaaaa atttattgaa gttgaaaagg aaataaaatc
    13501 aaaaaataag aaagaacata aaagtgagat ggtagaaata ccatttgaca aaattgaaaa
    13561 aactaacatt ctaatttcat tcaaatgatt acgcagatgg acagcctaaa tttaattgaa
    13621 acgtttgccg agttcgcgaa gggcaagaat attgatcggc caacaatgat cagaattctc
    13681 gaagatgttt tcagaaccat gatcaggaag aaatacgacc aggatgataa ctttgacatt
    13741 atcattaatg tcgataaagg tgatcttgaa atttggagat ttcgtgaaat tgtcgatgac
    13801 aattcggaag acatctggga ttatgataaa atcaccttaa cggatgcaag aaagatcgaa
    13861 gccgactttg aaatcggcga ggaagtagct gaagatatta aactcgaaga ttttggaaga
    13921 agagctgtta tgactgcacg tcaaactctg atgcaaaaaa taaaagacct tgaaaaagat
    13981 attctctttc ataagtataa agacctggta ggtgaaatta ttaatggaga ggtatatcag
    14041 attctttcaa gggaagttct gttgatagat ggtgagggca atgaacttac cattccaaaa
    14101 agtgaacaaa tccagaaaga caggtacagg aagggtgata ccgcccgcgc ggtggttcac
    14161 cgggtagaaa tggtcaatgg aaaccctaaa attattcttt caagaacctc ccctgttttc
    14221 ctggaaagac tttttgaaag cgaagttccg gaggtgtacg atgggttaat cactcttaag
    14281 aaagtagtta gggagcctgg tgaaagggca aaagtggctg ttgaatcgta tgatgacagg
    14341 attgacccgg tgggcgcctg cgtaggaatg aagggatcca gaattcatag tatcgttcgt
    14401 gaattgcaaa atgagaatat cgatgtaatc aacttcaccg aaaacctgga actctacatc
    14461 cagcgggcat tgagtcctgc taaaattagc agtataaaaa ttaatgcgga agaaaaaaga
    14521 gtttccgtat acctgaagcc ggatcaggtt tctctggcaa tcggtaaagg cgggcaaaat
    14581 attaaacttg ccagcaggtt ggtggatatg gaaattgatg tgttccggga aattggagat
    14641 tacgaagagg aggatgtgga tctggaagaa ttcggtgatg aaattgaaaa ttggataatt
    14701 gacgaattca agaaaattgg attggatacc gcaaagagtg tgctcgcatt atcaatggaa
    14761 gatcttatcc gaagaacaga tttggaagaa gaaactattg cagaagtagt gcgcatatta
    14821 aagaaggagt ttgagtaagt caatttttaa tactcataaa aattggggat atttgaagta
    14881 tggctgaaca aggcacaatg agattgggac aggtggctcg taagctaaat gttggtattc
    14941 ataccattac tgaacattta gcttccaaag gttttgaagt tgaagataag cctaatacca
    15001 agattacgcc tgaacaattc aatatcctgg ctaaagaatt tgcagcttct cttcttgaaa
    15061 aggaagaagc atccggcctt actattggcg cgaagcacaa tgaaaatgtc gtaattgaaa
    15121 aatcacggac agagcaaaga gaatcagaag atgacgatga aattctgatt aagaatttat
    15181 ccctcgaaaa agatggagag gtaactgatg ttggaaaaga aacagaagaa tctgaaaaac
    15241 cgaaattaaa aggtattacg gtagtcggta aaattgaact cggcgcaaag aaagaagaag
    15301 ccaaacagga atcagaagag aaggaaacga ttacaaaaga aacaagtgcc gggacaagag
    15361 aatccgggac cgagcctgtg cctgaatctg aattgattaa agcaaagtca gataaattgc
    15421 aaggccttaa agttgttggc aagattaatc ttccagccag aaaagagaaa gaaaaagaga
    15481 agtcagaacc ttttgcttct tccgacaaat ctgccgacag gaaaaagaga ccgcgaaagc
    15541 gaatcccgac tcaaaaaccg ccacagagaa caggagacag aaccaggaga agaccggaaa
    15601 aagaggagcc ttcagaaaaa gaaattcaag aacaaattaa agccacgctg gcaaaattaa
    15661 gcggtggcaa gaaaacctcc gccggagcca agtatcgcag agaaaaacgc atctcaaaag
    15721 cagaagccca cgaagaaaaa gttttgcagg aaatgagaga tagcaaagtc ctgaaagtga
    15781 cagagttcat ttcagccagc gatctggctt cacttatgaa tgttagcatt aatgatgtaa
    15841 tctccacctg cctgaatctg ggaatgttcg tttctattaa tcagcgattg gatgcggaag
    15901 caatcaccat catcgcagat gaattcaaat acagtgtgaa cttcaccacc agcgaagatg
    15961 aaactgaaat ttacgaagaa gaagatagtg aggaagattt aaagccacga gctcccattg
    16021 tcaccataat gggacatgtt gaccacggta aaacctcatt gctggattac atccggaaat
    16081 caaaagttac tgccggtgag gccggaggta ttactcagca tatcggcgcc tatgacgtat
    16141 taacagaagg tggacagaaa atcgcctttc ttgatactcc tggccatgaa gcattcactg
    16201 caatgcgcgc ccgcggagca aaagttacgg atattgttat tattgtggtt gctgcagatg
    16261 acaatgtgat gcctcagacg aaagaagcca tcaatcatgc tcaagtggct ggagttccaa
    16321 ttgttatagc tataaataaa attgataaac ccaatgctaa ccctgaaaag ataaaggaag
    16381 aactcagtaa ggtaaatatc ctggttgaag attggggtgg aaaataccaa tgccaggcta
    16441 tctcagctaa attggggcaa ggagtaaatg aattgcttga aaaggttctt cttgaagctg
    16501 aacttctgga gttaaaagca aaccatgata agcccgcgat tggaactgtg attgaagcct
    16561 ctcttgataa gggacggggt tatgttacta ctcttttggt tcaaaatgga aaactaaaaa
    16621 tcagcgacat cattctggca ggcccttatt acggaagagt aaaagccatg tttgatcata
    16681 ccggtacaaa aatgaatgaa gttggtcctt caactccggt tctggttttg ggattagacg
    16741 gcgctgccca ggcaggagac aaattcaatg ttatggagtc tgaacgcgaa gcacgggaaa
    16801 tttcggtgaa gcgtgagcag attttacggg agcaaaatat ccggactaag aagcatatta
    16861 cccttgatga aatcggaagg agacttgcca ttggcagctt ccgggaattg aatgttatag
    16921 ttaagggcga tgtggatggt tcagttgaag ctctttccga ttctttgttg aaattatcaa
    16981 ccggtgaagt tcagattaat atcattcata aaggcgttgg gcagatatct gaatccgatg
    17041 tccttcttgc cacagcctct gatgctgtaa ttgttggctt ccaggtgcgg ccttccgcaa
    17101 gtgcccgccg tctggccgaa tctgaggaaa tagaaattcg attatactcc attatttatg
    17161 acgccatcaa cgagattaaa gatgccatgg aaggtatgct tgctcctgaa gtggaagaag
    17221 taattaccgg taacgctgaa gtaagaagcg ttttcaagat tacaaaagta ggaacagtcg
    17281 ccggctgtat ggtaaccgaa ggggtgatca agaggaacag tgaaatcaga cttgtgcggg
    17341 atggtatcgt aatttatgaa ggacaaatcc tgcagctaaa aagatttaaa gatgatgcct
    17401 cagatgtaaa atcaggattt gaatgcggaa tcagtattaa gaatttcaat gacatcaaag
    17461 ttggtgatgt gattgaatcc tacgaaaaga aagaagttaa gaggaccttg taattttctt
    17521 tcatggaatt tgtccagaaa tttctattga cattaatcgg attgagcctg ttctttttag
    17581 tgctgggcct ttataaacct tactggatgg tgtggtggga agatattcag aacaggaaaa
    17641 aagtgttgtc cgtctacctt acttccgcga tatttttcct ttgcatttac cttgtcataa
    17701 gttacctgta ctaagttttt ctggagaaga aataatttct ttttttcttt atgaatcgcg
    17761 tactccaaat actgctcatc cttccttcat atacttccat cctactccgg atatcaatat
    17821 tctgatattc ggctcgtctg cacggttatt tttgtgatta aatgattatt ttgattttca
    17881 attacctccg ttcataacaa tagcaaaatc atccttgtaa tagttatttt tataaatcat
    17941 gacaaaatca aacaaatcat agtgcagaca accatagaag aagaaatgca attcgtatag
    18001 gccaacaaac gcattattga aatttttgag caaaaaataa aaactaaaat cagtgaggtt
    18061 tgaagtttag tactgagaat gcttcatact cccttgagaa tgaaaacttt ccatggcagc
    18121 agaaccttaa tggtatgata gaagaatttg aatataaagg aagatggagg gtgcctgatg
    18181 aagaattctg gtataatgga gctcttaaat tccatcctgg cgaacctatt attttggaaa
    18241 tattcggaac attcaatcct ggaatgttcg atacagaaaa aaagggaatt attttgggcg
    18301 aaacaacaaa aggggacatc acccttgttg atagtgatta cataacacat agccatagtt
    18361 ttgtttctga tgtaacaatt ggaaaatatc gcccaagtta tattatagct gggcatcaat
    18421 tctattccgc tgaatcaatt cagtttagta agactacgtt caaaacattc aatcttttta
    18481 catggctcga cacagcaggc cttatacgag acgaaagaaa cttacttcca agcgagtaca
    18541 ccaaacctga gtcgttatca tttaagtgtt ttgaagaatg tagtggtgct attgattttc
    18601 atcatactct aaatcttatt tttagaaata atcgaactga agttgaggaa gattgctcgg
    18661 tttcattaag ctataacaag agccgacatt ttaagaacat tttaatggac atctatatct
    18721 ttaatggctt cataacttta atgacttttg aacaatgtta tcctctttcc atttcattta
    18781 ctgacccaag ttatacggaa gataataaag ccaaaataat tcaatgtttt tatcaaaatc
    18841 ctgattacaa caaaaagtat aaagctcgga gtaaa
//
LOCUS       DASWHX010000199        11487 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_2456073, whole genome shotgun sequence.
ACCESSION   DASWHX010000199 DASWHX010000000
VERSION     DASWHX010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 11487)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11487)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11487
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2456073"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1321
                     /locus_tag="VGA21_13045"
     CDS             <1..1321
                     /locus_tag="VGA21_13045"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HEX9651483.1"
                     /translation="AGDKNEILKWEIENEYARRPESYGLPWIEIVPSVLTRPNKFSID
                     NYSGSLESWETFGKWLWDLNKDLDELPEATTRKISELVSSIEDPVEKIRAVYDYVQSN
                     TRYVSIQLGIGGWRPIAANLVDQMGYGDCKGLSNYTIALLKSIGIHANYVIIDSGWGE
                     DPLIKDFPSSQFNHVIVCVPLKEDSVWLECTSQTNPFGYLGTFTGDRAALSITEQGGK
                     IVHTVDYDQKLNQQNSFANVSIVASGNAIAKTKRFYHGLMYEKGGLDSYLNKNTTEQR
                     KWLNENIDIPNFEIIKFNFQNKKELIPVAIEEMELRLQRYAPVSGKRIFFKPNLFTKR
                     NIVRQQIRERKTDLLIRFGYVETDTIIFNLPKDYHPEFIPDPINLESEFGTYSFRVEI
                     ENSKLIYYRKASINKGRFSKEKYQDWIGFNKILADYDNMQIVLINRT"
     gene            complement(1323..2135)
                     /locus_tag="VGA21_13050"
     CDS             complement(1323..2135)
                     /locus_tag="VGA21_13050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020530870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9651484.1"
                     /translation="MKDKVVIITGGSSGIGKALAEIFGSKGSKIVITGRNSDALSKVV
                     FELEKKGIEILGVHSDVSKWEDNVAMVDQTLTRFEQIDVLINNAGINMRALFSDVDLE
                     VIKKVMDINFYGTIYATKACLPHILKNKGSILGISSIAGYRGLPGKTAYSTSKFAMQG
                     FLEVLRTELLYTGVNVMTVCPGFTASNIRKATLNKEGKLQGESPRNEDKMMTAEECAK
                     RIYKAAVNRRKIVIMTRQGKLAVFMNKWFPVWMDKKVYDLFADEPGSPLSQN"
     gene            complement(2141..5056)
                     /locus_tag="VGA21_13055"
     CDS             complement(2141..5056)
                     /locus_tag="VGA21_13055"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009580049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX9651485.1"
                     /translation="MKVSAAQPFQIVYSLYQHEYMGFLFESFVVHVDQYGKLTFQHQN
                     ISSQNAREFASGLDEKDFKLIELMDSIQQDAIVKKFSRKLIKPAEFFLKIYDKQRGNV
                     DVQNEIESFLEKRRAKILSLIKGRQMYEMGNDGEPTWRKIEITPKNASVLFHFRRNAD
                     NTHYFPTIKYNGEKLEWQYKGAYLICHNPAWLVLNNKLYGFQGEVDGYKLKPFLNKKF
                     IVIPRNLEDTYYKKFIAPLVASFKVFAKGFKIENEKLAPEPVLNFSEFGNGNTMDLFE
                     EMEESESDKIMFELSFRYGNYQFPADKIQPISVKLEKQGNDYTFHKVRRVNSEEKSAI
                     GFLSSIGMSLKNARAVMSKPEAFNWINDNLIPLEEKGFKINQKGANGKRYFLGESSIK
                     VQIDESIDWFDVNAIVKFGEFEIPFKELRKYITKNRREIKLPNGELAVIPESWFREYG
                     ELFAFVHLDVEKGTSHLYKHHLALISDLKKNQLAEVTISRKLEKLKNLESIGNFEMPL
                     HFTGTLRPYQKAGYDWLCFLNEFGFGGCLADDMGLGKTVQTLALLQARKSVADSASLL
                     VMPTSLLYNWEMEIKKFTPRLRIFIYTGTHRKKNPSVFEDFDIILTSYGIIRLDAEIL
                     SGYNFDYIILDESQAIKNPSSIVAKAVHQLNSKYKLILTGTPVENSTLDLWSQISFIN
                     PGLLGTMSFFKNEYMVPIEKKKDESKTQKLYSLIKPFILRRQKSQVAKELPEKVENIK
                     YSEMTARQAEMYDEVKSKFRNQILDTIEKQGLSQSQLLVLQGLTQLRQLANHPKMVDE
                     SFEGVSGKIEDVMYMLDNAQSEGHKTLVFSQFVKLLTIMRSELEQRGYKYCYLDGSTR
                     DRKSVVEQFQNDDSVRIFLISLKAGGLGLNLTEADYVFLLDPWWNPAVEAQAVDRAHR
                     IGQDKKVFTYKFITRNSVEEKILELQKKKLKLAQNLITKDENIYKSFSKEEIESILA"
     gene            complement(5127..6293)
                     /locus_tag="VGA21_13060"
     CDS             complement(5127..6293)
                     /locus_tag="VGA21_13060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010854608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX9651486.1"
                     /translation="MISRISMGGVAIGSMALIIVLSVFNGLEELLRTLYGTFDPEIKI
                     EVSEGKTFEITEELLEKVRSVPGINRVTESAEDFVYITYKNAEYAAILKGVSADFINE
                     EKFESAVVNGEFKLKEDDINFAIVGKGIQYALGMDPANLFEPLQLHYVRNAPSGAAID
                     PSRLIHKRSILPAGYFALEKQIDETYIIVPLEFALELFDFSSRRTALELRLLEDANIR
                     EVQSQLKSKLGPTFKILNSDEQHATLLRTVRIEKLFVFIIFTFIIAIASFNIFFSLSM
                     LAIDKKKDISILYSMGANDSIIRRIFLTEGAMIALSGASIGLILGAVITWIQQNYGII
                     SMGMQTSVLEDYPVKMKITDFIYTGASIVVITFLSSFRPALIATRYASLKSLHS"
     gene            complement(6365..6757)
                     /gene="rbfA"
                     /locus_tag="VGA21_13065"
     CDS             complement(6365..6757)
                     /gene="rbfA"
                     /locus_tag="VGA21_13065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014771422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEX9651487.1"
                     /translation="MEETTRQKKFARLIQKEVAEILLKDTKGIVGNSFVSVSYVSISP
                     DLALAKIYLSMLLEKDREAILEKIEFRKKEIRKMLGNKIGKQVRIIPELIFMVDDLEE
                     KAFKMDALIDSLEIPPPADESARDSEEE"
     gene            6871..8274
                     /locus_tag="VGA21_13070"
     CDS             6871..8274
                     /locus_tag="VGA21_13070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651488.1"
                     /translation="MLTFFKINDPYRVLFVFLGLILVWIPGLISNPLTLPELNWMITG
                     ERLGGGNILYRDLWTEVAPFSAGVYWVLDIFFGRSQAAYMILALVLISFQCVIFNRIL
                     LKNDSFRQPNYVPAVIYASLSILSFDFMTLSPQLMATTFLLLAMDFAFTHIMARVKQD
                     EKIQYIGLFLLAATLFYFPYFIFLIGFLLVLLIFTGTVARRFFLILYGYFLPLVLTGI
                     YYWYRDGLEYFWNQFLTMDFFQNERKLMSSNSMMLVAGMPLTFIIFSFIRLFGRTRYT
                     NFQITLIQVVLLLFIFAGLGYWISPTKPPHALIIFLPFAAFLISHFFMLIKSKFFSEM
                     AFLSFLVLIILWNFGTRYQFSPTSERIDYSDLKVSTSRWDEKASGKNIMILTPDINVL
                     NVSKLASPFLNWRVSKEVFENPDVYENVILVYKSLEKEKPDLIIDPQGVMGDFFKKMP
                     GLESKYNQAGEFWIRRN"
     gene            8334..8963
                     /locus_tag="VGA21_13075"
     CDS             8334..8963
                     /locus_tag="VGA21_13075"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020533978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HEX9651489.1"
                     /translation="MSFFTDEEWVKWFDCLSGESYLVIDNYLPEEIYRISRNYLISHF
                     ENDDFKKAGIGASTSHMVKDSVRGDFVYWLSRDKDVEFEQFFELADELKEKLNRYCYL
                     SLSGYEFHLAHYPPGAHYEKHIDEFHGRNNRMISVVIYLNEHWKPSDGGELKIYLSDK
                     EIIIEPISRRCVLFISDKVQHEVLTTKVSRYSLTGWLQHHPSILDHIMR"
     gene            9081..>11487
                     /locus_tag="VGA21_13080"
     CDS             9081..>11487
                     /locus_tag="VGA21_13080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651490.1"
                     /translation="MIKYMILGKLNCRELILSLAFIPGLLFLNNQTYGQNTSVAIGKL
                     QPQDNAVLLLFSPEKNQGLIIPVSYRSSIAAPEQGMIVFDDQEKKVFYFDGSNWKELG
                     APGLNSNSSSLHIELNGNNLEIYDGTSMVNSSSFTETAPVPDEVLTWDGDKWAPGQIT
                     ISPSKVVGEIAVGVVPKGSLSGLVESSILDYGNGVSILNNVFIEKALAVGSLSNFGLP
                     GQVLTSNGTGLAPAWTLISGDATLAGDGSFLISPGTINSTKILDGSISAADLGNMGAV
                     NGQVLKWNQTNNRWEPGSDNTGTGTVATLNNGEILLGDGTGNFALTMGGDAAINSATG
                     VVTISDEAITSAKIQDGTISTTDVGDAQITDAKINSVSAGKVTGITNNIVPRSNGAGL
                     VDGTIEDDGMNIAIGATINTQMKLRVRNTSSVNNYSYGIYSLVANAPGSNIGVLGYGI
                     SSTGSEARGLNGIASGTGLNIGVYGGTLSGGANSWAGFFAGKVLVETDLYIRNGIALG
                     SGNNFGTAGQVLTSNGAGFPVSWTTMSGGSGFSIDNAIPVGDGTGMVASQIVDDGAQL
                     GIGIAPDVAAKLSLSTDNHFFTLYAENERVTGSTNSGIVGSARNSTSGNVGVWGDAVV
                     PTSGDNIGIYGKAFGGNKNWAGYFEGDIATTGGLAFGAAQDFGTTGQVLTSNGANAAP
                     SWTTPGGSLPAISSGQLFVGDATNNPVAVNMSGDATISNTGVLTIAINAIGSAEITDG
                     SIATADLGNDVVTAAKILDGTITSSDISNATINGIDLAGNIDISTSGYITTSDLNVNS
                     VDYT"
BASE COUNT         3276 a         2330 c         2367 g         3514 t
ORIGIN      
        1 agcgggagac aaaaatgaaa ttttgaaatg ggaaattgaa aatgaatatg caagaagacc
       61 tgaaagctat ggattgccct ggatagaaat tgttccatcc gtgctaacaa gaccgaataa
      121 attttcaatt gacaattatt caggctcgct ggaaagctgg gagacttttg gcaaatggct
      181 ttgggatttg aataaagatt tggatgaact tccggaggca acaactagga aaatctcaga
      241 gttggtaagc tcaattgaag atccggtgga aaaaatcaga gcggtatatg attatgtaca
      301 atccaataca cgctacgtga gcatccaatt ggggattggt ggatggcgac ccattgctgc
      361 aaaccttgtt gaccaaatgg gttatggtga ttgtaaagga ctttccaatt atacaatcgc
      421 attactaaag tcgattggta ttcatgccaa ttatgttata atagattcgg gttggggtga
      481 agatccctta atcaaggatt ttccaagctc tcagtttaat catgttatcg tgtgcgtacc
      541 attaaaggag gatagcgttt ggcttgaatg cacaagtcag acaaacccat tcgggtatct
      601 gggtactttt actggagata gagcggcttt gtcaattacc gaacagggag gaaaaatcgt
      661 tcatacggtt gattacgatc agaaattaaa tcagcaaaat tcctttgcaa atgtttcaat
      721 tgttgcttcc ggaaacgcaa tagcgaaaac aaagcgtttc tatcacgggt taatgtatga
      781 aaaaggcggc ctggattcct atttaaataa gaacactacc gaacaaagga aatggctcaa
      841 tgaaaatatc gacatcccaa attttgaaat cataaaattc aactttcaga ataaaaagga
      901 attgatacct gtggcgattg aagaaatgga attgcgattg caaaggtatg ctcccgtttc
      961 cgggaaaaga atcttcttta agccaaacct atttacaaag cgcaatattg tacgacagca
     1021 aatcagggaa aggaaaaccg atttgctgat taggtttggt tatgtagaaa ctgacacgat
     1081 catttttaac ctgccaaaag attatcaccc ggaatttatt cctgacccaa ttaatctgga
     1141 atcagaattt ggcacttata gcttccgtgt agagattgag aatagtaaat tgatttatta
     1201 ccgcaaagcc agtataaata aaggtagatt ctctaaagaa aaatatcagg attggatagg
     1261 ctttaacaag attttggctg attatgataa tatgcagatt gttctgatta atcgaacgta
     1321 gctcaatttt gacttaacgg actcccaggt tcatcggcaa acaaatcata caccttttta
     1381 tccatccaca caggaaacca tttattcatg aataccgcaa gttttccctg gcgggtcatt
     1441 attactattt tcctccgatt tacggctgcc ttataaattc tctttgcaca ttcttcggcg
     1501 gtcatcatct tatcctcatt ccgaggagat tcaccctgga gtttcccttc tttattcagc
     1561 gtggctttcc tgatattgga agcggtaaat ccgggacaga ccgtcattac atttacaccg
     1621 gtatataaca gttctgttct taacacttca agaaatccct gcatggcaaa tttggaagtg
     1681 gaataggcag tttttccagg taatccacga tagcccgcga tagaggaaat tcccaggatc
     1741 gaacctttat ttttaagaat atgaggaagg catgctttgg tagcgtaaat ggttccatag
     1801 aaattgatat ccatcacttt cttaatcact tccaaatcaa catctgaaaa aagggctcgc
     1861 atattaattc ccgcgttgtt aatcaggaca tctatttgct caaatcttgt cagagtttga
     1921 tcaaccatgg caacattgtc ttcccacttg cttacatcac tgtgaactcc gagaatttca
     1981 atgccttttt tctccaactc aaaaaccact ttgcttaaag cgtcggagtt tctgccggta
     2041 ataacaattt tggagccttt acttccaaag atttctgcta aagctttccc gattcccgaa
     2101 gaacctccgg ttattattac taccttatct ttcattgcat ctaggccagt atcgattcaa
     2161 tttcttcctt tgaaaaagat ttataaatgt tttcatcctt ggtgattagg ttttgagcga
     2221 gtttcaattt cttcttttga agttcaagaa ttttttcctc tacagaattc cgggtgataa
     2281 acttgtaggt aaaaactttt ttatcctgac caatccgatg cgccctgtct acggcctggg
     2341 cttccaccgc cgggttccac caggggtcga gtagaaaaac gtaatccgct tcggtgaggt
     2401 tcaaacctaa tccaccggct ttcagggaaa tcagaaaaat cctgactgag tcgtcatttt
     2461 gaaattgctc aactactgat tttctgtcgc gcgtggagcc atccaggtaa caatacttgt
     2521 aaccacgttg ttccagttcg gagcgcatta tggtgagcaa ttttacaaat tgactaaata
     2581 caagggtttt gtgtccttcc gattgcgcgt tgtccagcat gtacattaca tcttcaatct
     2641 ttccggaaac accttcaaat gattcgtcca ccattttcgg atgattcgcg agctgccgga
     2701 gttgagtcag gccctgcaag accagtagtt gggattgact caacccttgc ttctcaatgg
     2761 tatcaaggat ttgatttctg aattttgatt taacctcatc atacatttcg gcttgcctgg
     2821 cagtcatttc agaatactta atattctcca ctttttccgg taactctttc gcgacttgtg
     2881 atttttgccg tctcaggata aatggtttaa tcagggagta aagtttttgc gttttcgact
     2941 catctttctt cttctctatc ggaaccatgt attcattttt gaagaacgac attgttccca
     3001 gcaaaccagg attgataaac gaaatctgag accagagatc cagcgtcgaa ttttccactg
     3061 gtgttcctgt caggatcaat ttatatttag aattaagctg gtgcacagct tttgccacga
     3121 tgctggatgg atttttaata gcttgtgatt catccaggat tatataatcg aaattatatc
     3181 cggaaaggat ttcggcatcc agccgtatta tgccgtagga ggtaagtatg atatcaaagt
     3241 cttcaaaaac cgaggggttt ttcttgcgat gagtgccagt gtaaatgaaa attctgagcc
     3301 gtggggtaaa cttcttgatt tccatctccc agttataaag cagggaagta ggcatcacaa
     3361 gtaaggatgc gctgtcggca acagattttc tggcctgtag cagagcaagg gtttgaacag
     3421 ttttgcccag tcccatatca tcggcaagac atccgccaaa gccaaattca ttcaggaaac
     3481 aaagccaatc atacccagcc ttctgataag gtcggagcgt tccggtaaaa tgcaggggca
     3541 tctcaaaatt tccgatggac tccaggtttt tcagcttttc aagctttctg ctgatggtca
     3601 cttcagcaag ctgatttttc tttaagtcgg aaatcaacgc caggtgatgt ttgtaaaggt
     3661 gcgatgttcc tttttcgaca tctaaatgaa caaaagcgaa aagctcgcca tactccctga
     3721 accaactttc gggaattacc gcgagctctc cattgggtaa ttttatctcc cgacggttct
     3781 ttgtgatgta ctttcgcaat tccttgaaag gaatttcaaa ctcaccaaac ttgactatgg
     3841 cgttaacgtc aaaccaatct atgctttcat caatttgaac tttaatcgaa gattcaccaa
     3901 ggaagtatcg tttaccattt gccccttttt gatttatctt gaatcctttc tcctccaacg
     3961 gaattaaatt atcatttatc caattgaagg cctcgggctt cgacattacg gctctggcat
     4021 ttttgagaga catacctata ctggagagaa atcctatcgc gctcttttct tcagaattta
     4081 ccctccgtac tttgtgaaac gtataatcat tgccctgctt ttccagtttt acacttatgg
     4141 gttgaatctt gtcagccggg aactggtagt taccgtacct gaacgaaagc tcaaacatta
     4201 tcttatcaga ttccgactct tccatttctt caaataaatc catggtatta ccatttccaa
     4261 actcggagaa atttaacacc ggctcgggag ctagtttttc attttcaatt ttgaaaccct
     4321 ttgcaaacac tttaaaagaa gcaacaagag gcgctataaa ttttttgtaa tacgtatcct
     4381 ccagattcct gggaataaca ataaatttct tattaagaaa aggtttaagc ttatacccat
     4441 ccacttcacc ctgaaatcca tacaatttat tattcagaac cagccatgca ggattatggc
     4501 aaatcaggta agctccttta tactgccatt caagtttttc accattgtat ttaatagttg
     4561 ggaagtaatg agtattgtcc gcatttctcc taaagtgaaa caacacactt gcgttttttg
     4621 gcgtaatttc tattttccgc catgtgggtt caccatcgtt ccccatttcg tacatctgtc
     4681 tgccttttat taaagagaga attttggccc ttcttttttc aaggaaagat tcgatttcgt
     4741 tctgaacatc cacattaccc ctctgcttat cataaatctt tagaaagaat tcagcgggtt
     4801 tgataagttt tcgtgaaaat ttcttcacaa tagcatcctg ctgtattgaa tccattaact
     4861 caattagttt gaagtccttt tcatccagtc ctgaagcgaa ttctctggca ttttgcgagg
     4921 aaatattctg atgttgaaag gtaagcttgc catattggtc aacatgaacc acgaaggatt
     4981 caaacaaaaa acccatgtat tcgtgctggt agagcgaata aactatttgg aacggttgag
     5041 cggcggaaac cttcattctt acaattggcc aatttgcaat gtaagaaatt ttggtctaaa
     5101 atccagtgat atttacctgc aagcccctaa ctatgaaggc ttttcaggga tgcataccgg
     5161 gtggcaatta gcgcgggacg aaatgaagaa aggaaagtta taactacaat actggcccct
     5221 gtatatataa aatcagtaat tttcatttta accggataat cttccaatac agaggtttgc
     5281 attcccatag agataatccc atagttttgc tgaatccagg tgataaccgc gccaaggatc
     5341 aaaccaatgc ttgctccgct taatgcaatc atggcaccct cggttagaaa aattcttcga
     5401 atgatggaat cattggcgcc cattgaatat aaaatggaaa tgtccttctt tttgtcaata
     5461 gcaagcatag acaaggaaaa gaagatattg aaagaggcaa tggctatgat aaatgtgaag
     5521 atgataaaaa caaaaagttt ttcaatcctg acggtgcgca acaaggtggc atgttgttcg
     5581 tcactattca ggattttgaa tgttggaccc aattttgatt ttagttgaga ctgcacttcc
     5641 cggatgttag cgtcttccaa aagtcgcaat tcgagtgcag ttcttcttga gctgaaatca
     5701 aaaagttcga gggcaaattc aagcggtaca ataatatagg tttcgtcaat ttgcttttcc
     5761 agtgcaaagt atcctgcagg aaggatggaa cgcttatgaa tcaaccttga tggatcaata
     5821 gcggcccccg atggagcatt tcttacgtaa tgaagttgca atggttcaaa taaatttgcc
     5881 gggtccatcc ccaatgcata ctgaattccc ttgcctacga tcgcgaaatt aatgtcatct
     5941 tcttttaatt tgaactcgcc gttcaccaca gcactctcaa acttttcttc gtttatgaaa
     6001 tcagcactca caccttttaa aatcgcggca tattcagcat tcttgtaagt gatataaaca
     6061 aagtcttcgg ccgattctgt tacacggtta atgccgggga cacttctgac tttctccagt
     6121 aattcttcag taatttcaaa tgtcttgcct tctgaaacct caatttttat ttctggatca
     6181 aaagttccgt aaagggttct tagcaactct tcaagtccgt taaaaacact tagcacaata
     6241 atcagggcca tagagccaat ggcaacaccg cccattgaaa tgcggcttat cactgtgata
     6301 aaatttcttt tcttttttga aaaaaaataa cgacgggcaa cgtagaaagg aaatctcatg
     6361 attattactc ttcttcggaa tccctggcag attcatccgc gggtggagga atttccagag
     6421 aatcgatcaa cgcgtccatt ttgaaagctt tttcttcaag atcatcaacc ataaaaatga
     6481 gctcaggaat tatccgcacc tgcttgccaa tcttattacc cagcattttc ctgatctctt
     6541 ttttcctaaa ctcgatcttt tccagaatcg cttccctgtc tttctcaagt aacatactga
     6601 gatagatctt cgcaagggca agatccgggc ttatggaaac gtatgacact gagacaaatg
     6661 aattccccac aatccctttt gtgtctttta ataaaatctc agcaacttcc ttttgtatca
     6721 gcctggcgaa ttttttttgt cttgtagttt cttccatttc acaaaaatag tattgttgaa
     6781 ctccttatcc tttaaattat ggaaaatacc tttttattac gctttaatta atctttattt
     6841 tcgcctgatt atcaggataa ccgtttcaaa ttgttaacat tttttaagat aaacgatccg
     6901 tacagggtcc tctttgtatt tctgggtctc atacttgtct ggattccagg cttgatatcc
     6961 aatcccctga cattaccgga attaaactgg atgattacgg gcgaaaggtt aggtggcggt
     7021 aatatccttt atcgtgatct ttggacggaa gttgctccct tttcagcagg tgtttactgg
     7081 gtattggata ttttcttcgg aagatctcag gccgcctata tgatacttgc cctggttctc
     7141 ataagttttc agtgtgtcat ttttaaccgg atactcctta aaaatgattc tttcaggcag
     7201 cctaactatg tcccggcggt tatatatgca tcattgtcga ttctctcctt cgattttatg
     7261 acgctttcac ctcaattgat ggcaacaacc tttctgctgc tggcaatgga ttttgctttt
     7321 acccatatca tggccagagt aaagcaagat gagaagattc agtatatcgg gctattcctg
     7381 cttgcagcaa cactgtttta ttttccatat tttatctttc taataggatt tctgctggtg
     7441 ctcttgattt ttaccggcac tgtggcaaga agatttttcc tgatcttata tggatatttt
     7501 ttaccgctgg ttttaacggg aatatattat tggtacaggg atggtcttga atatttctgg
     7561 aaccagtttc taactatgga tttttttcaa aatgagagaa agctaatgtc gtcgaattcc
     7621 atgatgttag tggctggcat gccgctaaca ttcataattt ttagtttcat ccggctcttt
     7681 ggcagaacac gctatacaaa ctttcagatt actctcattc aggtggtttt gcttttattc
     7741 atttttgccg gcttaggata ttggattagc cctacaaagc ctccgcatgc attaatcatt
     7801 tttctgccct ttgctgcttt tttgatcagt cattttttca tgctgataaa atccaaattt
     7861 ttttcagaaa tggcatttct ctcatttctt gtgctgataa ttctatggaa ttttggaacc
     7921 cgctatcaat tttcgccaac ctcggaaagg atagattaca gtgacctcaa agtttcaaca
     7981 tcaaggtggg atgaaaaggc ttcgggtaaa aatataatga ttttgactcc tgatataaat
     8041 gtattgaatg tgagcaaact tgcaagtcca tttttgaact ggagagtctc taaagaagtt
     8101 tttgagaatc ctgatgtgta tgaaaatgtc atcctggttt ataaaagcct cgaaaaagaa
     8161 aaacctgacc tgataataga ccctcagggt gtaatgggtg acttcttcaa aaaaatgccc
     8221 ggtctggaat caaagtataa ccaggcggga gaattttgga taaggagaaa ttgagtttgg
     8281 ggtaagtttg aatttggatt tggtgtggtg ggaaatttgt taattttgtt ccgatgagct
     8341 ttttcacaga tgaggagtgg gtcaaatggt ttgattgcct ctcaggtgaa agttatttgg
     8401 taattgataa ttatcttccg gaagaaatat accgtatttc ccgaaactat ttaatttctc
     8461 attttgagaa cgatgatttc aaaaaggccg gcattggcgc atcgacttct cacatggtga
     8521 aagactcagt tcgtggagac tttgtctatt ggctatcgag ggataaagat gttgagtttg
     8581 aacaattctt cgaacttgct gatgaattga aagaaaagct gaatcggtat tgttacctca
     8641 gtctttccgg gtatgaattt catcttgctc attatcctcc cggtgcgcat tatgaaaaac
     8701 atattgatga atttcacggt cggaataaca ggatgatatc ggtcgtaatt tatcttaatg
     8761 agcattggaa gcctagcgac ggcggagagt tgaaaatata tctgtcggat aaagagataa
     8821 tcattgaacc tatttcacgt cgctgtgtct tatttataag cgacaaggtg caacacgaag
     8881 tgctcaccac aaaagtatcc cgctatagct taaccggttg gttacagcac catccctcaa
     8941 ttttagatca tattatgagg tgaatcactc aaaaggtgtg atttcttgtt caataatacc
     9001 ggttcgtgta tgaaattata taaaataaca aataatatat attacctaat atatatattt
     9061 ttgtgtcaag taagactata atgataaagt atatgatatt aggaaaatta aactgcagag
     9121 aacttatact aagcctggct tttattcccg gattactctt tctcaataat caaacatatg
     9181 gacaaaacac ttccgtagcc ataggcaaat tgcaacccca ggacaacgcg gtgcttcttc
     9241 ttttttcgcc tgaaaaaaat cagggactca ttatccctgt gtcttacagg agttcgattg
     9301 cagcccccga acaaggcatg atcgtttttg acgatcagga gaaaaaggtc ttttattttg
     9361 acgggagcaa ctggaaagaa ctgggtgccc ctggattaaa ttccaattca agttctcttc
     9421 acattgaatt aaacggaaac aacctggaaa tatatgacgg aacctcgatg gtaaattcat
     9481 cttcatttac agaaactgca ccggtgcctg atgaagtttt aacgtgggat ggcgacaaat
     9541 gggctcccgg acaaattaca atttctcctt ccaaagtagt gggtgaaata gctgtcggtg
     9601 ttgttcctaa gggcagtttg tcaggccttg tggaaagctc gattcttgac tatggaaacg
     9661 gtgtctctat tctgaataac gtgttcattg aaaaagcact tgcagtggga agtctttcaa
     9721 actttggtct tcccggccag gtacttactt caaacggaac tggtttagct cccgcgtgga
     9781 ctttgatcag cggagacgca actcttgccg gtgatggaag ttttcttatt tctcccggca
     9841 caattaattc aacaaaaatt ttagacggta gtatttctgc cgctgacctt ggtaatatgg
     9901 gcgcggtaaa tggccaggtt ctcaaatgga atcagactaa caatcgctgg gaacccggat
     9961 ctgataacac cgggacagga acggtagcga cgctgaataa cggggaaatt ttgcttggtg
    10021 atggcaccgg caattttgcc ttgacaatgg gcggcgatgc ggccataaac agtgcaacag
    10081 gagttgtaac gatttcagat gaggcaatta catccgcgaa aatccaggat ggaacgatct
    10141 ccacaacgga tgtgggtgat gcgcaaatta cagatgctaa aatcaattcc gtttcggcag
    10201 ggaaggtgac tgggattaca aataacatcg tgccaaggag caatggagca gggcttgttg
    10261 atggaacaat tgaggatgac ggtatgaata tcgcaattgg cgcaactatt aatacacaaa
    10321 tgaaattacg agtccgaaac acaagttcgg ttaacaacta cagctatgga atttattcgc
    10381 ttgttgcgaa cgcaccgggt agtaatatcg gtgtattggg ctacggtatt tcttcgactg
    10441 gttcagaagc caggggcctc aacggtattg catcaggtac aggtcttaat attggggttt
    10501 atggtggaac tctttcaggt ggagcgaata gttgggctgg attttttgcc gggaaagtgt
    10561 tagtcgagac agacctgtat atcagaaatg gaattgcgct cggtagcgga aacaatttcg
    10621 gcactgcagg ccaggttctc acttccaacg gtgccggttt tcccgtgtcc tggacgacca
    10681 tgagtggtgg cagcggcttc tcaatagata atgcaattcc ggttggagac gggacaggaa
    10741 tggtagcttc ccaaatcgtt gatgatgggg cgcagttggg cattgggatt gctcccgatg
    10801 tagcagcaaa attatcactg tcaacagaca atcatttttt cacactatat gcggagaatg
    10861 aacgcgtcac aggttcaacg aattccggta tagttgggtc tgcaagaaat tcaacatccg
    10921 gtaacgtggg agtttggggg gacgcggttg tccctacatc cggagacaac atagggattt
    10981 acggaaaggc ttttggaggt aataaaaatt gggcaggata ttttgagggc gatatcgcca
    11041 caaccggcgg acttgctttt ggagcagccc aggattttgg cacaacaggg caggtactca
    11101 cctcaaatgg agccaacgct gcaccatcgt ggacaacacc tggtggcagc cttcccgcaa
    11161 taagttcggg ccagctattc gtaggagacg caaccaataa cccggttgcg gtaaacatga
    11221 gcggtgacgc gacaatcagt aacaccggtg tcctgactat tgccattaat gcaatcggtt
    11281 ctgctgagat caccgacggc tcaatagcaa cggcagacct tggcaacgac gtggttactg
    11341 cagcaaaaat ccttgacggc actatcactt cttccgacat ttcaaacgcg acgattaacg
    11401 gtattgatct tgccggtaac attgatatca gcacaagtgg ctacatcacc acttccgact
    11461 tgaacgttaa ttcagtcgat tatacat
//
LOCUS       DASWHX010000200        12599 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_589885, whole genome shotgun sequence.
ACCESSION   DASWHX010000200 DASWHX010000000
VERSION     DASWHX010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 12599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12599
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_589885"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="VGA21_13085"
     CDS             <1..180
                     /locus_tag="VGA21_13085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HEX9651491.1"
                     /translation="SEFLIPFAVDNLIQESKCLTKVIRTDARWFGITYKEDKPLVENA
                     IRELIDKKVYPTPLW"
     gene            174..1172
                     /locus_tag="VGA21_13090"
     CDS             174..1172
                     /locus_tag="VGA21_13090"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HEX9651492.1"
                     /translation="MVITKIEEIAGKFFTGKFDFELLETGHINQTFLVSNKSGVKFIL
                     QSLNTHIFKHPRFVVDNYMTIQPFCHDIVPKLIQTKNGKLFLTIENNTWRAFEYVEGT
                     RTIQEIQNEDQAYSGARLFGIFLHKCAIINPEDIAETIPQFHNLNNRIKKFNTAYKED
                     KLNRGSHIRKLIPVLDKFHFLGNEFQKATESLPQRVVHNDTKPGNLLFASETNEAKKV
                     IDLDTVMPGLIITDFGDMVRSFTPGGKEDDPNPESAEVRRSIYQALTEGFSESVQTFI
                     LPEEKELLTFGAQCIIFEQALRFLTDFLEGDLYYKTSKENQNLDRGIHQLNILKSV"
     gene            1403..3259
                     /locus_tag="VGA21_13095"
     CDS             1403..3259
                     /locus_tag="VGA21_13095"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HEX9651493.1"
                     /translation="MKFSWKKLILGSLLTIVLVVTVFLVNLIWFKPFSIRLFYDKIFV
                     EFAISDPEVVTQMAIPVLYDRTKSDLTDVSDARQWENFNKMKETLATLRSYNFENQSD
                     ENKLNTRILEWYLAVQAESEAYFYYDYPVNQMFGVQNNLPSFLENSHKMRDESDIEAY
                     ISRLSKFEIKFDQVLEGLHIREDRGIIPPKFMITRVIDEMKGFTGQNESIALEEDPNP
                     VKRNILYTNLNKKISEVEGLTEEQKQEFMTRVEEEIRSTVFPAYQKLIDYFSELYQKA
                     NTDDGVWKFSDGENFYRYMLKQNTSLNLDPAEVHAMGLSEVERIKQEMWNILISEGYQ
                     DTTQNIGDVIQELNKQERFLFPNDDNGRQAALNEYNLILDEISAGLNDAFDIRPKAGL
                     EVLRVPEFKEEGAAGAYYNRPAMDGSRGGLFYANLRDMKEVVKFGMKTLAYHEGIPGH
                     HFQVAIQSELEGQPIFRTISLFTAFTEGWALYAERLAWELGFYENDPFGNLGRLQAEM
                     FRAVRLVVDTGIHFKKWTREEAIDYMVANTGMTPSGVTTEIERYIVMPGQACAYKIGM
                     MKILELREKAKSELGEKFDLRKFHNVVLKNGAVPLEILEDLIDGYIAESKGA"
     gene            complement(3323..4435)
                     /locus_tag="VGA21_13100"
     CDS             complement(3323..4435)
                     /locus_tag="VGA21_13100"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX9651494.1"
                     /translation="MRKFAALIILIAVSCTSKVEESTSSLDILDKKFSAFFPEGEPGG
                     AVLLAKDGEVIFSKGYGIADINTKEPITAKTLFNLGSISKTFVSNAILILEEQGMLSI
                     DDELIKYFPEFKNPEIGSKVRIKHLLTHTSGLPDNRKVNEDTVFYLTAKDAENWAPEL
                     EVDSLNFEPGTNFEYSNPAFNGLALIIEQVSGIKWQDFIIENIMRPSGMVASTITDGA
                     HPESGVSHGYEKYLGKFIEDDYGEEPTFAAAGNGGVWSSVEELFQYELALESSKFLSK
                     EKMQDSRIAKKFRKWIGEEPENIGWSWFVTKTEEGLPMVGHTGSQGGFRANYQTIPEK
                     EIFFVILCNTPRDGFSEIMMGWLRENNWLDGMPEKN"
     gene            complement(4463..5287)
                     /locus_tag="VGA21_13105"
     CDS             complement(4463..5287)
                     /locus_tag="VGA21_13105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019987991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HEX9651495.1"
                     /translation="MSRIIRVIFIVLLSLMVFVWLWKSGDQKIETPPGMVFIPGNDAM
                     DSFFMDATPVTVAQFREFVNATGYITQAEKFGDAGVFTIEENWHLVKGANWEFAMGRE
                     EPKAKDSHPVTQISYYDALAYSEWAGKRLPTEKEWQHAAMDGEHLDTQYNWGNRLVVE
                     GRFMANTWQGSFPFYNSNEDGFATTSPVGTFGKTKLGLTDISGNVWEWCSDWKISPGL
                     SMVNFEPDSTTEKVLRGGSFLCDLSYCQGYRLDGRSGSTPQTSLFHTGFRCVKDIN"
     gene            complement(5305..7476)
                     /locus_tag="VGA21_13110"
     CDS             complement(5305..7476)
                     /locus_tag="VGA21_13110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014772957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEX9651496.1"
                     /translation="MKRNLLNILLFHLALILTIGCAPSVTENESNSFQPPVAEKIEQK
                     LEANGDVRLDNYYWMKLTDEQKNAEAPDEQTQKVVKYLEDENEYTAAVLNGTEEFQKK
                     LFDEIVSRIKQTDESVPYFENGYWYYTRYEEGKEYPIYCRKVETMDNSEEIMLNVNEM
                     AEGYAYYAVGGRSVSTDNNLLAYAVDTVSRRQYTVYFKNLETGEIADLYIPNTTGGVT
                     WANDNETVFYTKKDPITLRSDKIFRHEVGTNAANDAEIYYEGDDTFYTGVYKTKSDKY
                     IIIWSGSTLTNEYRIIDANNPAKPFRVFSPRERGLEYNIDHYKNKFYIVTNLDALNFR
                     LMETPENATSKENWKEVIPHRADVLLQGVEVYNDYMVISERSKGLTQLRIINQNDKSE
                     HYLDFGEQAYTAYVSTNPEFDSKILRFGYTSLTTPNSTYDYNMETREKILMKQEEVLG
                     GFDPGNYVTERIWATARDGVKVPISLVYRKGFVKDGNAPMQLYGYGSYGATIDPSFSS
                     IRLSLLDRGFVYAIAHIRGGQMLGRQWYEDGKMFKKKNTFTDFIDCAKFLIEENYTGT
                     EKLFAYGGSAGGLLMGAVANMSPETFKGIIAAVPFVDVMSTMLDESIPLTTGEYDEWG
                     NPNELESYQYMKSYSPYDQVEAKYYPNMLITTGYWDSQVQYWEPAKWVAKLRDLKTDE
                     NKLLLYTNMDVGHGGASGRFERYKLTALQYAFMFDILGITE"
     gene            7610..8917
                     /locus_tag="VGA21_13115"
     CDS             7610..8917
                     /locus_tag="VGA21_13115"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX9651497.1"
                     /translation="MKRLILLIPVLLFNASTNPEKSVVVEKELIKKDLPYLVDFYKTR
                     HMNPEISLEEKNTSAALAAELRKIGIEVTGNFGGYGIVGVLKNGDGPTILYRTDMDAL
                     PMYEKTELPYKSKLITQYSGEEVGTMHSCGHDMHMAAWLGTARVMTEMKSRWKGTLVL
                     IGQPAEEIGQGSKMMLDAGLYQKFGVPDYGIGLHSSPTIAAGQVGLGKGFTMANTESI
                     DIKVFGVGAHGATPHMSIDPVIIGSMIVMELQTIVSRSLKPTESAVITVGAIKGGIKH
                     NIIPDEVTLKLTVRTFTEEVRMMVHKRIKEICRGVAIAAGLSEDNLPEVIIPDVFTPS
                     NYNNPDLVDRITQSAESVISKDNVVYAEPQMVGEDFSRYGQTEHKVPTVLLWLGTVPD
                     DRMKSGFLPGLHSPFYYPQPEKSIETGVSVMSQTLLDLFNSGS"
     gene            complement(8901..9416)
                     /locus_tag="VGA21_13120"
     CDS             complement(8901..9416)
                     /locus_tag="VGA21_13120"
                     /inference="COORDINATES: protein motif:HMM:NF042765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6702 family protein"
                     /protein_id="HEX9651498.1"
                     /translation="MPGLLVPILPIFFSFSGNVLSEVNHAIYLSVCEVDHREQGNSAE
                     INVKVFETDIRDAFQNMFSLQADFNSARATSAYKEESEQYFKKYLKIVINGQRADLKF
                     SSLELNGDSVWFYFTITCNKEWASISVTADYLMELFPDQSNIISVYHGDDKKFFRITR
                     TKKTDQASFRN"
     gene            9541..9948
                     /locus_tag="VGA21_13125"
     CDS             9541..9948
                     /locus_tag="VGA21_13125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651499.1"
                     /translation="MIDDLSKFMKKVSEQIEAKFLEYDQERSVVICPVKGGRIQTVYG
                     TIKSGDKYGGKTGIEFTSKVCSLMDVQNPLDMIKAQSENCHAKFVVYNDEVWVEACAY
                     LESATESTLREMVMETAQMADTWEMKVTGLDVN"
     gene            complement(9945..10844)
                     /locus_tag="VGA21_13130"
     CDS             complement(9945..10844)
                     /locus_tag="VGA21_13130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008198035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX9651500.1"
                     /translation="MRRFFLYSIIIASINSCTAETEDPKSRLENNIQARLNQIKGIFA
                     VAFKDLNTGDTLFINADEGFHAASTMKTPVMIEVYKQADAGLFSMEDSIEVKNEFVSI
                     VDGSLYSMDISDDSEPELYAKIGSKIPVRDLVIPMITYSSNLATNILIDKVGAKNVTA
                     SMRVFGAMKIEVLRGVEDIKAYELGMSNTTTARDLQIILEAIAKKTAGLEESCNEMLD
                     ILLQQKFNEIIPAKLPEETEVAHKTGSITGVQHDSGIVYLPDGTSYVIVLLSKELENA
                     NDGIAALAEVSALVYEFVVKEKN"
     gene            complement(10955..>12599)
                     /locus_tag="VGA21_13135"
     CDS             complement(10955..>12599)
                     /locus_tag="VGA21_13135"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX9651501.1"
                     /translation="FKQEFLFQPLLNIHLYSNLLQESQPQEQGDGQAVYFLGIVAFFI
                     LIIAWVNYINLSTAKSFERANEVGVRKVMGAVKSQLRNQFIIESFLINLMALVIAILI
                     IILAWPSFTQLTGRPIPFTMIYEPSFWLLATGLFAGGTLLSGFYPAWMLSSFKPIAVL
                     KGKSTIQSGGSFLRKGLVVFQFFASVTLISGTLIVWDQLRFMKNMDLGIDIRQTLVLE
                     SPGMRDSLYSQNVDAFKNEVLRINGVANVTVNTNVPGDEIFYTRGIRRLTGGPESHLT
                     SYIVGIDENYIPAFNLNVIAGRNFSKDFPSDEDGIIINRSLANSIEYVNLEEAIGEKV
                     SLGGDTLNIIGVIEDYHQMSLKNKTAPIVMRYSTFDSFYALKINSDSYQSVLAGVNGV
                     YNQIFPGNPVNYFFLDEFFNKQYDSDRQFGQVFSLFSILAIFVACLGLFGLASFMTTQ
                     RTKEVGIRKALGSSVGGIAALLSGSFIKLVIVGAILATPVAWIIMSLWLESFPYHIKI
                     SPLVFLLSWLMVILIALASVAYQTITTALLNPAKTLRYE"
BASE COUNT         3813 a         2635 c         2498 g         3653 t
ORIGIN      
        1 tcggaatttc tgattccttt tgccgtcgat aatctcattc aagagagcaa atgcctaaca
       61 aaagttatca gaacagatgc ccgatggttt ggtataactt ataaagagga taaacctctg
      121 gttgaaaatg caattcgtga actcattgat aagaaagttt atccaacccc cttgtggtaa
      181 ttacgaaaat tgaagaaata gccggaaagt tttttaccgg taaatttgat tttgaattgc
      241 tggaaacagg tcacatcaat cagaccttcc tggtgagcaa taaatctggt gttaaattca
      301 ttctacaatc tcttaacact catatattta aacacccccg gttcgttgtt gacaactata
      361 tgactatcca gcccttctgc catgatatag ttccaaaatt aattcaaact aaaaacggga
      421 agttatttct taccatcgaa aataatacct ggcgagcatt cgaatatgta gaaggcacaa
      481 gaacaatcca ggaaattcag aatgaagatc aggcatattc cggagcccgg ttatttggca
      541 tttttcttca taaatgtgcc ataataaatc cggaagatat tgcggaaaca attcctcagt
      601 tccataatct gaataacagg ataaagaaat ttaacacggc atacaaagaa gataaattaa
      661 accgcggaag ccatatcaga aaattaattc cggttttgga taaattccat ttcctgggca
      721 atgagtttca aaaagcgaca gagtctcttc cgcaaagagt tgttcataat gataccaaac
      781 caggcaatct cctatttgca agtgaaacga atgaagcaaa aaaggtcatt gatcttgaca
      841 ctgtaatgcc aggcctgata ataaccgatt tcggagacat ggttcgcagc tttacccccg
      901 gtggaaaaga agatgatccg aatcccgaaa gcgctgaagt aagaaggagc atctatcagg
      961 cgttaacgga aggattttcc gaatccgttc aaacatttat actcccggaa gaaaaggaac
     1021 ttctaacctt tggagcccaa tgcataatct ttgaacaagc tttgcgattc ctgactgatt
     1081 ttcttgaagg agatttgtat tacaaaacaa gtaaggagaa tcaaaatttg gataggggaa
     1141 ttcaccagtt aaatatttta aagtccgtgt aacattaaac atgctccgcc agtccgtgga
     1201 ttacgtttgt atttttggca gggtcttagt ttggaatcat tatacgtgtg ccacactttt
     1261 tcgacatgga aaacaatcaa tttcacaaaa taaagacaga agtgtggcaa acgtagatat
     1321 taatttcaat gtgatcttaa ctgaaacact accgtatttt tgaaggttta ggtacattta
     1381 ataaaacaaa attcaattta ttatgaaatt cagttggaaa aaattgatcc tcggctcctt
     1441 actcactatc gtgcttgttg tgaccgtttt cctggttaat ctaatctggt tcaaaccgtt
     1501 cagcatccgg cttttttatg ataaaatctt tgttgaattc gcaatcagcg acccggaagt
     1561 ggtaactcaa atggcaatcc cggtgctgta tgacagaact aaatccgatc tcaccgatgt
     1621 ttctgacgcc agacaatggg aaaattttaa caagatgaaa gaaaccctgg cgacgctgag
     1681 aagttataat tttgaaaatc agagtgatga aaataagctg aataccagga tacttgaatg
     1741 gtaccttgcg gtccaggctg aatctgaggc gtatttttac tatgattacc cggtaaacca
     1801 gatgtttggt gtacaaaata accttcccag ctttctggaa aactcgcata agatgagaga
     1861 tgaaagcgac atcgaggcct atatttcccg gttgtcaaaa tttgaaataa aatttgatca
     1921 ggttcttgaa ggattgcaca tccgtgaaga taggggaatt attccaccca aattcatgat
     1981 caccagagtc atagatgaaa tgaaaggttt tacaggtcaa aacgagagta ttgccctgga
     2041 ggaggatcct aatccggtta agagaaatat tttgtatacc aacctgaaca agaaaattag
     2101 tgaagttgaa ggattgacag aggaacaaaa gcaggaattt atgacccggg ttgaggaaga
     2161 aataaggagt actgtattcc ctgcttacca aaaactgata gattatttca gtgaactcta
     2221 ccaaaaagcg aacacagatg atggcgtttg gaaattctct gatggcgaaa atttttaccg
     2281 gtacatgcta aagcaaaaca cctcccttaa ccttgatcct gctgaagttc acgcaatggg
     2341 attatctgaa gttgagcgaa taaagcaaga gatgtggaat atattaatca gtgaggggta
     2401 tcaggatacg acacaaaata tcggtgatgt tatccaggaa ttgaataagc aggaacgctt
     2461 tctttttcca aatgatgata acgggcgaca ggcagcgttg aatgaatata acctgatact
     2521 ggatgaaatt tctgctggcc tcaatgatgc gttcgatatc agaccaaagg caggattgga
     2581 ggtgctgcgg gttcccgaat ttaaggaaga aggagccgcc ggtgcttatt acaatcgccc
     2641 cgcgatggat ggctcacgcg gtggattgtt ctacgcaaac ctcagggata tgaaggaggt
     2701 agtaaaattc ggaatgaaaa cgctcgctta ccatgaggga attccgggac atcattttca
     2761 ggttgctatt cagagcgagc tggaaggcca acccattttc agaaccattt ctttgttcac
     2821 tgctttcacc gaaggatggg cgctttacgc ggaaagactt gcctgggaat tgggtttcta
     2881 tgagaatgac cccttcggaa atctgggccg gctgcaagcg gagatgttca gagcggtaag
     2941 gctggttgtt gatacaggaa tccattttaa aaaatggacc cgggaggaag caattgatta
     3001 tatggttgca aataccggga tgactcccag cggtgttacc actgaaattg aacgatacat
     3061 tgtaatgccg ggtcaggcat gtgcttataa gattgggatg atgaaaattc tggaattacg
     3121 ggaaaaggcc aagagcgaat tgggagagaa gtttgactta agaaagttcc ataacgtagt
     3181 gctgaaaaat ggcgctgtac ctttagagat tcttgaagat ctaatagatg gatatattgc
     3241 cgaatcgaaa ggggcgtgat tttgttaagt gtccggtgat taaattttca attattatga
     3301 ttgatccggg atttcaaaaa agttaatttt tctccggcat cccatccagc caattatttt
     3361 cacgtagcca gcccatcata atttccgaaa aaccgtcgcg cggagtattg caaagaatta
     3421 caaaaaatat ttccttctca ggaatagttt gataattggc gcggaacccg ccctgactgc
     3481 cggtgtgacc aaccattggc aatccttctt cagttttcgt aacaaaccat gaccagccaa
     3541 tgttttccgg ctcttcacca atccatttcc taaacttctt tgcgattctt gaatcctgca
     3601 ttttttcttt ggaaagaaat tttgagcttt ccaaagctaa ttcatattga aacaattctt
     3661 caacagaact ccaaacccca ccgttgcctg ctgcagcgaa agttggctct tctccataat
     3721 catcttcaat aaattttcca agatattttt catagccgtg acttactcct gactccggat
     3781 gtgcaccatc tgtaattgta ctggccacca tgcccgaagg ccgcataata ttttcaataa
     3841 taaaatcctg ccattttatt ccgcttacct gttctatgat tagggccagt ccattaaacg
     3901 caggattcga atactcgaaa tttgttcccg gttcgaagtt cagactatcc acttcaagct
     3961 caggagccca gttttcagca tctttcgccg ttaagtagaa cacagtgtct tcattcactt
     4021 ttctgttatc aggcagtccg gaagtatgag taagcaagtg tttaattcta accttggaac
     4081 caatttcagg atttttaaat tcgggaaaat actttatcaa ctcatcatct atcgacagca
     4141 taccctgctc ttcaagtatc agaattgcat tggagacgaa agttttcgaa atagaaccta
     4201 aattgaaaag agttttcgcg gtgattggct ccttcgtatt tatatccgct attccatatc
     4261 ctttggaaaa aatgacttcc ccgtcttttg ccagcaatac cgcgccaccc ggttcaccct
     4321 cagggaaaaa tgcggagaat tttttatcaa ggatatccag ggaagaggtt gactcctcaa
     4381 ccttagaggt gcaggatacg gcaatcagga taatgagtgc agcaaatttt ctcatattct
     4441 caggtttttc tcaaattcaa gttcaattaa tatctttaac acatctgaaa cccgtgtgga
     4501 aaagacttgt ttgcggagta gaaccactcc ttccgtccaa cctatatccc tggcaatagg
     4561 aaagatcaca caagaacgaa ccgcctcgca aaactttttc tgtggtgcta tcaggttcaa
     4621 aattcaccat tgataatccg ggagaaattt tccaatctga acaccactcc caaacattac
     4681 cggaaatgtc cgtcaaacca agttttgttt taccaaaggt accgacggga gacgtagttg
     4741 caaaaccatc ttcatttgaa ttataaaatg gaaaggaacc ctgccaggtg ttagccatga
     4801 atcttccttc caccaccagt ctattgcccc agttatactg tgtatccaaa tgttctccat
     4861 ccattgcggc atgctgccat tctttttcag ttggaagccg ttttccagcc cattctgaat
     4921 aagcaagagc atcgtaataa gatatctgag ttacagggtg gctatccttt gctttcggtt
     4981 cttccctgcc catggcgaat tcccagttag ctccttttac gagatgccag ttttcttcta
     5041 tagtaaaaac acccgcatcg ccaaacttct cggcctgggt aatatatccg gtagcattaa
     5101 caaattcgcg aaattgagca acggtaaccg gagttgcatc cataaagaat gaatccatgg
     5161 catcgttacc gggaatgaaa accattcccg gcggcgtttc aattttctga tccccgcttt
     5221 tccataacca tacgaaaacc attaatgaca ggagtactat aaatatgacc cggataattc
     5281 tgctcatgtt ctaaaaaatg actactattc cgtaatgccc agaatatcaa acataaacgc
     5341 gtattgaaga gctgttaatt tataccgctc gaacctaccg gaagctcctc catgtcccac
     5401 atccatattg gtgtaaagca acaatttatt ttcatcggtt ttcaagtcac gtaattttgc
     5461 cacccacttt gcaggttccc aatattgcac ttgtgaatcc cagtaaccgg tggtaatcag
     5521 catgttggga tagtatttcg cttcaacctg atcataaggt gaataggatt tcatgtattg
     5581 ataggattca agttcattgg gattccccca ctcatcatac tcacctgttg tgagcggaat
     5641 gctttcatca agcatggtag acattacatc cacaaatggt acggcggcaa taataccttt
     5701 aaaggtttca ggactcatat tggcaacggc tcccattaaa agaccgccag cgctgcctcc
     5761 atacgcaaac agtttctcag taccggtata attttcttcg attagaaatt ttgcacagtc
     5821 tataaaatcg gtaaatgtat ttttcttctt aaacatcttc ccgtcttcat accattgtct
     5881 tcccagcatt tgaccaccgc gaatatgcgc gatggcataa acaaatccac ggtcaagcaa
     5941 acttaatctg atggaactaa atgacggatc gatagtcgct ccgtaagatc catacccata
     6001 cagttgcatg ggcgcgtttc cgtctttgac aaaacctttt cggtatacaa gcgatattgg
     6061 cacttttacg ccgtcacgcg ccgtagccca aattctttcc gtgacataat ttccgggatc
     6121 aaatccacct aaaacctctt cttgtttcat taagattttt tccctggttt ccatattata
     6181 atcataagtc gaatttggag ttgtcagcga ggtatagcca aatcgaagaa ttttggaatc
     6241 aaattcagga ttagtagaaa cataagctgt gtaggcttgc tcaccaaaat caagataatg
     6301 ctccgattta tcattttgat tgatgatgcg taattgtgtc aggcctttgc ttctttctga
     6361 aatcaccata taatcattat atacctccac gccctggagc agtacatctg cgcggtgcgg
     6421 aatcacttct ttccagttct cttttgaagt agcattttcc ggagtttcca ttaatctgaa
     6481 attcagcgca tccaggttgg taactatgta gaatttgttt ttataatgat caatgttgta
     6541 ctccaaaccg cgttccctcg ggctaaaaac ccggaacggt ttagcaggat tattcgcgtc
     6601 aataattcta tattcattgg taagcgtact gcctgaccag ataattatat acttgtcaga
     6661 ttttgtttta taaactcctg tataaaacgt gtcatcaccc tcataataaa tttcggcatc
     6721 atttgccgcg ttggttccta cctcatgacg aaatatttta tctgaacgaa gggttatcgg
     6781 gtcctttttc gtgtagaaaa ccgtttcatt gtcattggcc cacgttacac ccccggttgt
     6841 atttggtatg tacaaatccg ctatctcacc tgtctccaga tttttaaaat atacggtata
     6901 ctgccggcgc gaaacggtat caaccgcgta agccaacaga ttattatcag tacttacact
     6961 tcttccacca accgcgtaat aggcatatcc ttctgccatc tcgttcacat taagcataat
     7021 ttcttctgaa ttatccattg tttccacttt tcggcaataa atcgggtatt ccttaccttc
     7081 ctcataccgt gtgtaatacc aatacccatt ttcgaaataa ggaacagatt catcagtttg
     7141 cttgattctg gaaacaattt catcaaatag tttcttctga aattcttcgg ttccatttaa
     7201 tactgccgcg gtgtactcat tttcatcttc aaggtacttt accacttttt gagtttgttc
     7261 atccggagct tccgcgttct tttgttcatc ggtcagcttc atccaatagt aattgtcaag
     7321 tctgacatcc ccattagctt caagtttttg ctcaattttt tcagcaaccg gtggttgaaa
     7381 tgaattcgat tcattttctg ttacagatgg cgcacatcca attgttaaaa tcaacgccaa
     7441 atggaacagc aagatgttta ataggtttct tttcatatgt ttaattttta cggggattaa
     7501 tttaatacaa ataactatga agattagagc catttatata aaatccccga tccttccatt
     7561 tgtggagcta tattattttc tttaacttga ataacataaa taactcccca tgaaacgcct
     7621 gattctctta attcccgttt tattgttcaa tgcatccaca aatcctgaaa aatcggttgt
     7681 ggttgagaag gaattaatca aaaaggactt gccttacctt gttgattttt ataagaccag
     7741 gcatatgaat ccggaaattt cattggaaga aaaaaatact tcagcggccc tcgcagctga
     7801 acttaggaag attgggattg aggtaaccgg gaattttggc ggttatggca ttgtgggtgt
     7861 tctcaaaaac ggagatggcc cgacaattct ttaccggacc gatatggatg cgctgcccat
     7921 gtacgaaaaa acagaactcc catacaaaag caaacttatc acccagtata gtggagaaga
     7981 agtaggaacc atgcattcat gtggacatga tatgcacatg gctgcatggc ttggaaccgc
     8041 aagagtaatg acggaaatga aaagccgatg gaaaggaacc ctggtgctta tcggacaacc
     8101 ggctgaagaa atcggacagg gttctaagat gatgctggat gccggccttt atcaaaagtt
     8161 cggtgttccc gattatggca ttggattgca ttccagtcca accattgccg ctggacaggt
     8221 cggtttaggc aagggtttta caatggcaaa tacggaaagc attgacataa aagtttttgg
     8281 agtaggagcg cacggggcaa cgccacacat gtctattgat ccggtgatta ttggaagcat
     8341 gattgtaatg gaactgcaga ccatagtaag cagaagcctt aaaccaacgg agtcggcggt
     8401 tattacagtt ggcgcaatta aaggaggaat caagcacaat atcattcctg atgaagttac
     8461 acttaaattg acggtgcgaa cttttactga agaagtgaga atgatggttc acaaacgaat
     8521 aaaggaaatt tgcagaggcg tggctatcgc tgcaggactt tcggaagata atttgcccga
     8581 agtaataatt ccggatgttt ttactccttc aaattacaat aaccctgatc tggttgacag
     8641 gattacccaa tcagcggaat cagtgattag taaggataat gttgtctatg ccgaacccca
     8701 gatggttggc gaagatttca gccggtatgg acaaaccgaa cacaaagtgc ccacagtgtt
     8761 attatggctg ggaaccgttc ccgatgacag gatgaaatca ggattcttac caggactgca
     8821 ttctcctttt tactacccac agccggaaaa gtcaattgaa accggtgtga gcgtgatgtc
     8881 tcaaacgtta cttgatcttt tcaattccgg aagctagcct gatcagtctt tttagtcctg
     8941 gtgatccgga agaatttctt gtcgtctcca tggtaaacag aaataatgtt agactgatcc
     9001 ggaaataatt ccataaggta atcggcggtt actgatattg aagcccattc cttattgcat
     9061 gtgatggtga aatagaacca tactgagtca ccatttagct ccagggaact gaatttcaaa
     9121 tctgctcttt gtccgttaat cacaattttc aggtatttct taaaatattg ttcagattcc
     9181 tccttgtagg ctgaagtcgc gcgtgcagaa ttaaaatccg cttgcaaaga aaacatattc
     9241 tgaaaggcat cgcggatatc cgtttcgaag acttttacat tgatctctgc cgagttacct
     9301 tgctcccggt ggtcaacttc acacaccgac aggtaaatcg cgtgattaac ttcagaaagg
     9361 acatttcctg aaaaactgaa aaatatgggt agaattggaa ccagtaagcc cggcatgata
     9421 caaatttaga aaacttcgcg cttatatttt ttccgggaat gggtagtttt tatttccctg
     9481 ataaatgaat tcaagtctgc atttttagta atttggcact gaagctaaac tagttgacca
     9541 atgattgatg acttgtccaa gtttatgaag aaagtctctg agcaaattga ggcgaagttc
     9601 ttagaatatg accaggagcg ttccgttgtc atatgtcctg taaaaggcgg cagaatccaa
     9661 actgtttatg gtaccattaa atcaggagat aaatatggcg gaaagacagg tattgaattc
     9721 acctccaagg tttgctccct aatggatgtt caaaacccat tggatatgat taaggctcaa
     9781 tcggaaaatt gccatgccaa atttgtagtt tataatgatg aagtttgggt tgaagcctgc
     9841 gcttaccttg aatcagccac agaaagtaca ttgagggaaa tggtaatgga aaccgcccag
     9901 atggcagaca cctgggagat gaaggttacc ggtttagatg tgaactagtt tttctctttc
     9961 accacaaact cataaacaag ggcagaaact tccgccaggg ccgctattcc atcattcgcg
    10021 ttttcaagtt ccttggaaag caagacaatt acgtaacttg ttccatcggg tagatacacg
    10081 atccctgaat catgctgaac tcctgtaatt gaacccgttt tatgggccac ctccgtttcc
    10141 tccggcaatt tcgcggggat tatctcatta aatttctgct gaagcaatat atcaagcatt
    10201 tcattgcacg attcctccaa accggcggtc tttttcgcga ttgcttccag gataatttgc
    10261 agatcgcggg ccgtagtagt attactcata cccagttcat aggcttttat atcttcaaca
    10321 ccccgcaata cttcaatttt catcgcgcca aaaacacgca tggaagcagt gacgtttttg
    10381 gcacccactt tatcaatcag aatattggta gcaagattac tgctatacgt aatcattggg
    10441 attactaaat ccctgacagg aattttggag ccaatctttg catataattc cggttcgctg
    10501 tcatctgaaa tatccataga ataaagactt ccatccacga tacttacaaa ctcatttttc
    10561 acttcaatag aatcttccat agaaaaaagt cccgcatcag cctgcttata tacttcgatc
    10621 atcacgggcg ttttcattgt actggcggca tgaaaacctt catcagcatt tataaataaa
    10681 gtgtcaccgg tattcaaatc cttaaaggct acagcaaaga ttcctttaat ctggttaagc
    10741 cttgcctgga tattattttc taacctcgat tttggatctt cagtctctgc cgtacaggaa
    10801 tttatgctcg caataatgat tgagtataag aagaatcttc tcatggaaat attattttga
    10861 attccaaagt aatggaaatc aggagtaaaa tcctaccaca acaatgttga ttgccaggtt
    10921 actatcttat aatggaggta ttaatttatc ttcattattc ataccgaagt gttttggccg
    10981 gatttagtaa ggccgtggta atagtctggt atgccacaga agccaatgca attaggatta
    11041 ccattaacca gcttagcaga aaaaccagcg gacttatttt gatatgatag ggaaatgatt
    11101 cgagccatag agacataatg atccaggcta cgggagtagc gagtatagct cccactatta
    11161 ccagcttgat gaaactacct gacagcaaag ctgcaattcc tccgaccgat gatcctaaag
    11221 ccttccggat ccctacctct ttggttcgct gtgttgtcat gaaggaagca agtccgaata
    11281 aaccaagaca cgctacaaaa atggccagga tggaaaacaa actgaaaacc tggccgaatt
    11341 gacggtcgga atcatattgc ttattaaaaa attcatcaag gaaaaaataa ttaacgggat
    11401 ttcccggaaa gatttgatta taaacaccat ttactccggc caaaacactt tggtaggagt
    11461 ctgaattaat ttttaaagca taaaatgaat cgaatgttga atatcgcata actataggag
    11521 cagttttgtt cttcaaagac atttggtgat aatcttcaat tacaccaatt atgttaagcg
    11581 tatcacctcc taaactcact ttctcgccaa ttgcctcctc gagatttaca tattcaattg
    11641 agtttgccag agatcggtta attattattc catcctcatc tgaaggaaag tctttgctaa
    11701 aatttcttcc cgcgataaca ttaagattaa aagcgggaat atagttctca tcaattccca
    11761 ctatataaga agtgaggtgt gattcagggc cacctgttag tcgtcggatt cccctggtgt
    11821 agaaaatttc atcacctgga acattggtat taacggttac attggcaact ccattaattc
    11881 tcagcacttc attcttaaaa gcatcaacat tttgactgta taaggagtcc cgcatcccgg
    11941 gtgactccag gaccagagtc tgacgaatat caatacccag atccatgttc ttcataaagc
    12001 gcaattgatc ccaaacgatt aaagttccac tgattagggt aacagaagca aagaattgaa
    12061 aaaccaccaa tccttttctt aaaaacgatc cgccactctg aatagtagat ttacccttga
    12121 gcactgcaat aggtttaaaa gatgaaagca tccatgcagg gtaaaagccg gaaagtaaag
    12181 ttccgcctgc aaataatccg gttgccaaca gccagaaaga aggctcataa atcatcgtga
    12241 atggaattgg ccttcccgta agctgggtga aagagggcca tgccaggatt atgatcaaaa
    12301 tcgcaataac cagtgccatt agatttatta agaatgattc tatgataaac tgatttctaa
    12361 gctgcgactt tacagcaccc ataacctttc ttactccaac ttcattggca cgttcaaagg
    12421 attttgctgt ggacaagttt atataattta cccaggcaat tatcaggata aaaaatgcga
    12481 ctattcccag gaaataaact gcttgtccat caccctgttc ctggggctgg gattcctgca
    12541 acagatttga atacaaatgg atattcagca aaggctgaaa aagaaattcc tgcttaaaa
//
LOCUS       DASWHX010000201         5868 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_590781, whole genome shotgun sequence.
ACCESSION   DASWHX010000201 DASWHX010000000
VERSION     DASWHX010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 5868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5868
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_590781"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(433..2106)
                     /locus_tag="VGA21_13140"
     CDS             complement(433..2106)
                     /locus_tag="VGA21_13140"
                     /inference="COORDINATES: protein motif:HMM:NF024579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651502.1"
                     /translation="MINVSFKRLSVPGWAGTFRYHPVWLLTIVLSILSCTPKSENAGF
                     AAKTGITVCGTVQFSDGCGQETDSLISYGLALVHHMTYDEAQKVFDKVIATAPECFWG
                     HWGKALTLIHPVWPDEPGEAGMKQGWELIQKASQLSSNQKETVYGQALASYYENGLEK
                     TELERLKSFEKGWETALEMFPDDLEIKSFYALLLISTADPQDKTYPNLIKAGKLAEEV
                     LQVIPDHPGGFHYTIHAYDYPELADKALSAANNYSDIAPEVPHALHMPTHIFTRLGMW
                     EESIDWNLRSAKAASKQRVGGNVSLHYFHAVDYIVYAHLQRLEDLKASQIIGEFKNAE
                     APYQEHSTTAYTMASVEARYALERQEWEKAASLEARQPNGINWDKFPEFEALTHFAVG
                     LGAARSGLTKKSENAAKKLEELKQKVSNPYWAQQVEIQKNAVKGWLAYANGNKKEALE
                     WMTLAAEQENATNKHAITPGELLPASELLGDMLLELNKPAEALVQYEIALKRSPLRFN
                     SLYGAAHAAELSGNQEAAKTYYEKLIEVSADAEVTLPRREKAMSYLRGA"
     gene            complement(2178..2558)
                     /locus_tag="VGA21_13145"
     CDS             complement(2178..2558)
                     /locus_tag="VGA21_13145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651503.1"
                     /translation="MEQEFQSIIIIPLGDELRATIIKFIEEAVAKAWYHDSNKNQRGA
                     ISNIILDKFDTGQLKDFVERLKRSNTKTINQFLANHTPLNHQDEEINKIAKLQKSKET
                     PGKRLKTQERYKIFFEVYYGNNPN"
     gene            2749..3387
                     /locus_tag="VGA21_13150"
     CDS             2749..3387
                     /locus_tag="VGA21_13150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651504.1"
                     /translation="MRRRLFFLKFLKLIAFLGFGSSFFLKRTVSGENELTRSFIRYRD
                     NPNDDTAYHLYKTLPLYNSNVKQSRQEIDNLYYQSFIVLKNHLATGLKGAIDLAFRLF
                     SITYGAERSALIWAIAPCIHINPTYLLVSLKTHSHLVSKEMLLGNLGLEFVDNIALQD
                     LEIERRILSLENVNDPSLLRIRNECIGILANKHHDACDYMMEVGKYNISKSL"
     gene            complement(3616..3714)
                     /locus_tag="VGA21_13155"
     tRNA            complement(3616..3714)
                     /locus_tag="VGA21_13155"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3653..3655),aa:Asn,seq:att)
     gene            3848..4198
                     /locus_tag="VGA21_13160"
     CDS             3848..4198
                     /locus_tag="VGA21_13160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651505.1"
                     /translation="MKSFGQTARDFRIEKGFTLRNFCSKANLDPSNWSKVERELMEPP
                     KDKEIIETIASALNLDNEQKQKLMDLAIIHSIPKELVDEQIVKKLPIFFRTLSGKKPT
                     EKELKELIKLLSKP"
     gene            4206..4817
                     /locus_tag="VGA21_13165"
     CDS             4206..4817
                     /locus_tag="VGA21_13165"
                     /inference="COORDINATES: protein motif:HMM:NF017885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEX9651506.1"
                     /translation="MPKPRRLTQRYICDEADKTRGSEDIPVEIEEIIELKYKIQIVPL
                     RELLKVVNTEGFLSKDKLEIYVDQERYINPIFKQRLRFTLAHELGHYILHPEFYEDVE
                     FKKPEDIITFFNSMNEDDLHWFEYQANEFAGRILVPVTKLESLINGNKSYIEELKGKF
                     QESDGHDDLILDAFSRKICGDFDVSNDVIKIRIHKERLIEHFS"
     gene            5227..>5868
                     /locus_tag="VGA21_13170"
     CDS             5227..>5868
                     /locus_tag="VGA21_13170"
                     /inference="COORDINATES: protein motif:HMM:NF024502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase SAM-like domain and Arm
                     DNA-binding domain-containing protein"
                     /protein_id="HEX9651507.1"
                     /translation="MINVTTGIILDTRRIKKDGSYPAKLRICHLRKRKYYSIGKSYTE
                     TDFVKIEDKKSRGWHKEERVILNAITAKAEDAIKKIEQFSFSAFERKFFISKTDRESV
                     YFHFQEYIDFLNKQARVNTADSYRSALNSLIAFKRSLSFNEVTVEFLNRYEAWMKSED
                     RSPSTVGIYIRSLRTIINNAIKQGDLVMSNYPFGKHGYQIPNSRNIKKALRLRE"
BASE COUNT         1771 a         1119 c         1152 g         1826 t
ORIGIN      
        1 ttttaagagt ttgggcaaat tatttaacaa tgattaaatt cagaatttta attacttcat
       61 tatcacgatt ccttcgcttc gcataccttt tcatttgaag tttgttaatc tcatattttt
      121 caaaagccat tcgaaaaatg aactctacct ctccctgccg ccgcaaaagg tgtacctgaa
      181 aaattgaaag agacttgcta ccaaaatgat cggcggaaga ggaaaagttc ataaagccat
      241 cagggaagcc ctggaaaaag acgccgaact aaaggcccga tatgaggcaa tcctggctga
      301 tgtgaaagat cccaacaaaa gttaattttc aggaattgca gttgttttaa atataataaa
      361 agaggaggga ggacctcctc ttttattatt gtccacagga attctttcct taaaccggaa
      421 atcaaagatc gtttatgctc ctctcaggta tgacattgct ttttctctcc ggggtaaagt
      481 gacctcggca tcagccgaaa cctcgatcag tttttcgtaa tacgtttttg ccgcttcctg
      541 gtttccggaa agttcagcag catgggctgc accataaagg ctgttaaacc tgagtggact
      601 tcttttcagg gcgatctcat attgaacaag cgcttcagcc ggtttgttga gctccaacaa
      661 catgtcaccc agtaactcgc tggccggaag taattctccg ggagtgatag catgcttatt
      721 tgtggcattc tcctgttcgg cagccagggt catccattca agcgcttctt ttttatttcc
      781 atttgcatat gccaaccagc cctttacggc atttttttgg atttcaactt gctgtgccca
      841 atatggattg cttacttttt gtttaagctc ttccaatttt ttagccgcat tctcagactt
      901 tttagtgagc ccgctccttg cagcgcccaa tccgacagca aaatgggtca gagcttcaaa
      961 ttccggaaac ttatcccagt ttattccgtt gggttgccta gcttcaagag aggcggcctt
     1021 ttcccattcc tgtctttcca gggcataccg ggcctcgacg gaagccatcg tataggcagt
     1081 ggttgaatgt tcctggtatg gagcttctgc attcttaaac tccccgataa tctgacttgc
     1141 ctttagatct tccagacgtt gtaaatgcgc ataaacaata tagtcaacgg catgaaaata
     1201 gtgcagtgat acattgccgc caactcgctg ctttgaggcg gccttcgccg atcgcaaatt
     1261 ccagtcgatg gattcttccc acatacccaa ccgcgtaaaa atgtgggttg gcatgtgcaa
     1321 tgcatgaggg acttcaggag caatatcgct atagttgttg gcggctgata aagctttatc
     1381 cgcgagttcg ggatagtcgt aggcatgtat ggtgtaatga aatccgccgg ggtgatcggg
     1441 aatgacctga agtacctctt cagccagttt accggccttt attaaatttg ggtaagtttt
     1501 atcctgcgga tcagcagttg aaataagcag taaagcataa aaagatttta tttcaagatc
     1561 atccggaaac atttccaaag cagtctccca gcctttctca aaactcttta acctttccag
     1621 ttctgttttt tcaaggccgt tttcatagta agaggcaaga gcctgaccat aaacggtttc
     1681 tttttgattg gatgaaagtt gagacgcctt ttgtatcaat tcccatccct gcttcattcc
     1741 tgcttctcca ggttcgtcag gccaaaccgg atggatcaag gttaacgctt ttccccagtg
     1801 tccccaaaaa cactccggag cagttgcaat tactttatca aaaacttttt gagcttcatc
     1861 atatgtcatg tgatgcacta aagccaatcc gtatgagatc agggaatcag tttcctgccc
     1921 acatccgtct gaaaactgaa ccgttccaca aaccgtgatg cccgttttag ccgcgaaacc
     1981 tgcattttca cttttcgggg tgcatgataa gattgacaaa acaatggtta ataaccatac
     2041 aggatgatat ctgaatgttc cggcccagcc tggaacagaa agacgtttaa atgaaacatt
     2101 tatcatatta tttaggtatt aggttgttta aactgacctg cagagggtaa acttggccaa
     2161 tccggtaaat ttgtgaatta attcggatta tttccatagt aaacttcaaa aaaaatttta
     2221 tatctctcct gggttttaag cctcttgcca ggtgtctctt tgcttttctg caacttggca
     2281 attttattaa tttcctcatc ttgatggttc agtggtgtat ggttcgccag gaattgatta
     2341 atggtcttag tatttgatct cttgagtctt tcgacaaaat cctttaattg acctgtatca
     2401 aacttatcta gaattatgtt tgatatggcg cctctttgat ttttatttga atcatgatac
     2461 catgctttgg caacagcttc ctcaatgaat ttaattatcg ttgcccgtag ttcatctccc
     2521 agagggataa taataatact ttgaaattcc tgctccaaaa acaaaagttg ttctcatttc
     2581 gaaaaaaatg attactgcta caacggatcg taaaagtaaa ttagtaaaat ctgcggggtg
     2641 cttgcaagta ttttgtcgca tcgtatcaga gagcttgtaa tagatatttc aatagtacaa
     2701 ttaccataat aattgactag ctttgactct agaatattcc ttggactaat gcgtagaagg
     2761 ttatttttcc tgaaatttct taaacttatt gcttttttag gttttggttc ttcttttttc
     2821 ttgaaaagga ctgtttcagg ggaaaatgaa ctaacacggt catttatccg gtacagagat
     2881 aatccgaatg atgatactgc atatcacctc tataaaactc tacctttata caattccaat
     2941 gtcaaacaaa gtaggcaaga gattgataat ttatattatc agagctttat tgttttgaaa
     3001 aatcatttag cgacgggtct aaagggtgca attgaccttg ctttcaggtt attttccatt
     3061 acttatggag cagaaaggtc agcacttatt tgggctattg ctccatgtat ccacataaac
     3121 ccgacatacc tcttagtatc tctgaagaca cattcccact tagtttcaaa ggaaatgctt
     3181 ttgggaaatt tgggtcttga atttgttgat aatatagcgt tgcaagattt ggaaattgaa
     3241 aggaggattc tgtccttaga aaatgtcaat gatccgagtt tattaagaat acgtaatgaa
     3301 tgtattggca ttttagctaa taaacaccat gatgcatgcg actacatgat ggaagtggga
     3361 aagtataata tatctaaaag tctgtaggtt aaatccttct taataatggg tctgaattgc
     3421 aacggccgat cttcctcttt tttctgattt gggaatttcc tcctcttaaa ggataatcct
     3481 attggattta cctttctcac cacggaacct catatttctt tcttctcccc gtgcttgaag
     3541 agaatcgtcc gaaaactacg gccagcatga aatcttgatc gcaaaaagtg cattattgag
     3601 attatgctgt gatgatgggc ccagcaggat ttgaacctgc gaccctctga ttaatagtca
     3661 gataatgaac aaacaaactt tctgataatc agactgatag caacttttct tcccttcagg
     3721 tcggatatag gatctgttct attctttggt ctttctgtga aactacaggc attaaaagat
     3781 ttcataatgt ggtattctct taccacaatt ttgtggtata tttgtaccac aactaaacta
     3841 aacgcaaatg aaatcattcg gtcaaactgc aagagacttt cggattgaaa aaggttttac
     3901 gctccgcaac ttttgttcaa aggcaaacct ggatccttca aactggagta aagttgaaag
     3961 ggaattaatg gaacctccaa aggacaagga aattattgaa accattgctt ctgctttgaa
     4021 tcttgataat gagcagaagc aaaagttaat ggaccttgca attattcatt caattcctaa
     4081 agaattggtt gatgaacaaa ttgtcaaaaa acttccaata ttttttagaa ctctaagcgg
     4141 taagaaaccc accgaaaaag aattgaagga gttaattaaa cttttaagta aaccctaaat
     4201 tcgaaatgcc gaaaccgaga agattgactc aacgctacat ttgtgatgaa gccgataaaa
     4261 ccagaggatc agaagatata cctgttgaga tcgaggaaat tatagaatta aaatataaga
     4321 ttcaaattgt tcctttacgc gagctcctta aggtggtaaa tacagaggga tttctaagta
     4381 aggacaaact ggaaatctat gttgatcaag aaaggtacat aaatcccata tttaagcagc
     4441 gacttagatt cacccttgct catgagcttg gccattacat tttacatcca gaattctacg
     4501 aagatgttga attcaagaaa ccggaggata taattacatt tttcaattca atgaatgaag
     4561 acgacctgca ctggtttgaa taccaggcaa atgaatttgc cggtagaatt ttagttccgg
     4621 taaccaaact ggagtcgctt ataaatggaa ataaatccta tatagaagaa ttgaaaggca
     4681 aatttcaaga atcagatgga catgatgatt taatcctgga tgcattctca cgaaaaattt
     4741 gcggagactt tgatgtctct aatgatgtaa tcaaaattag aatccataag gaacgattaa
     4801 ttgaacattt cagttagttt cagcaagatg aaagatcgag ttacaacctt cgttttagga
     4861 gcgccatcaa aagggttagg gattttaaaa gaagtagttt ttttcctgag gtcttgattc
     4921 tagtcattca tcagagtcct ctttgactct gatgggatta tttggaagac tcttttggga
     4981 aatggagaaa acaattgatg attcttaatt gctaattgaa attgttttcc tggtgggcca
     5041 gcaggatttg aacctgcgac cccctgatta tgagtcataa tccatactat ttgtttatta
     5101 tatctttctt accaattttg catctggaat tagtaaaaat agccatttta ggtcattata
     5161 ctattttttc ttttattgat gttattggtt tgttttgcta tatttgtgta tcaaattgtg
     5221 tagcaaatga taaatgtaac cactggaatc atcctggaca ccagaagaat caagaaggat
     5281 ggctcatacc ctgctaaact ccgcatttgc catttaagga agcgtaaata ctactccatc
     5341 gggaaatcct atactgagac ggattttgtt aaaatcgaag acaagaagtc cagaggatgg
     5401 cataaagaag aaagggtaat attaaacgca attacggcta aggctgaaga tgctattaag
     5461 aagattgagc agttttcatt cagtgccttt gaaaggaagt tctttataag taaaacagat
     5521 agagaaagcg tgtatttcca ttttcaggag tatatagact ttttgaataa acaggcgagg
     5581 gtaaacacag cggatagcta ccgatctgct ctaaactccc tgatagcctt taagcgaagt
     5641 ctttccttta acgaagttac ggtagaattt ctgaacaggt atgaagcctg gatgaaatca
     5701 gaagataggt caccttcaac agtaggaatc tatattagga gtttgcgaac catcataaac
     5761 aatgcaataa agcaaggtga tttggtgatg agcaactatc catttgggaa gcatggatac
     5821 caaataccca attccaggaa tataaagaaa gcacttagac tgagggag
//
LOCUS       DASWHX010000202        34055 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_592433, whole genome shotgun sequence.
ACCESSION   DASWHX010000202 DASWHX010000000
VERSION     DASWHX010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 34055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34055
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_592433"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="VGA21_13175"
     CDS             complement(<1..242)
                     /locus_tag="VGA21_13175"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX9651508.1"
                     /translation="MWNIFVISYFVLHSLCLIILLFNWRRIKFNKKVDEYNDFISVII
                     ACRNEEENITKIIESINLQDYPVKNFEVIIVDDHST"
     gene            324..884
                     /gene="ruvC"
                     /locus_tag="VGA21_13180"
     CDS             324..884
                     /gene="ruvC"
                     /locus_tag="VGA21_13180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015263952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEX9651509.1"
                     /translation="MTKVNNNEKIIVGLDPGTRIMGYAALLVQNSRLKLLQYGVIRLE
                     KYSGYELKLKKIFERVTGLIEEFSPDEIALEAPFYGKNVQSMLKLGRAQGVAMAAALH
                     HQIPIAEYAPKKVKQSVTGNGNASKEQVAKMMQTLLNIKETPKLLDATDALAVAITHY
                     FQARPKKGTSKSWKAFIADNPGRIKS"
     gene            complement(895..1290)
                     /locus_tag="VGA21_13185"
     CDS             complement(895..1290)
                     /locus_tag="VGA21_13185"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009583543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEX9651510.1"
                     /translation="MATIFTKIVNREIPAHIVAETNHFLAFLDINPLVMGHVLVIPKK
                     EVDYIFDLDSETLSGLMEFAKKVSSAIREAVPCLRIGVAVIGIEVPHCHIHLIPLNSM
                     NDINFTRPKLKPTDDELAEIASKIKSNLN"
     gene            complement(1294..1764)
                     /gene="greA"
                     /locus_tag="VGA21_13190"
     CDS             complement(1294..1764)
                     /gene="greA"
                     /locus_tag="VGA21_13190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009186697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEX9651511.1"
                     /translation="MGKISYYTEEGLKKLKEELSELKTKGRSEIAKQIADARDKGDLS
                     ENAEYDAAKDAQGLLELKISKLETILGNARILDQSTVDKSKVSILSSVTIQNKNNGSK
                     FTYTIVAEEEADLNEGKISVDSPIGKGLLGKKVGQSASVQVPAGKINFKILDIS"
     gene            complement(1892..2749)
                     /locus_tag="VGA21_13195"
     CDS             complement(1892..2749)
                     /locus_tag="VGA21_13195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013455308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEX9651512.1"
                     /translation="MAKQFFRLYETLSKISEKPILTSFIVLLLTAILVIGMSLPYYLG
                     DFKNFYAQILAEAHGMIFDIAVIGILIFWLNRRGEVRQRIRTYRDEIDDFRLWESEEA
                     AFRTVGNIKRLNRHNIFRIDLVETYLARTNLNHIDLEESNLNSANIRNSFLIQANLKK
                     TRLNQTCFENANLNQADLSDSYAGGANFKDAFLIKTNLRNSFLIKANFVNAFLMEADL
                     SGTHLTGANLENASLYKADLRSATGITVEQLSKAKTLYQAKLDPEIAIEMELQFPELL
                     GIERPMKVG"
     gene            complement(2843..3775)
                     /locus_tag="VGA21_13200"
     CDS             complement(2843..3775)
                     /locus_tag="VGA21_13200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008626654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9651513.1"
                     /translation="MKCIIIDKMHPSIAPALEGLGLEVDYFPEINQSEILQIIGTYEG
                     LIVRSKVSVDKTFLDQAPNLKFVARAGAGTDNLDTDELETRGIKIINAPEGNRDTLAE
                     HTVGLLLSLLNKIHLASHEVKSLQWNREANRGEELMGKTVAIFGYGNMGQAVAHRLKS
                     FGVEIIAYDKYKNEIAEDFVRLVPLKTIFNKTDVLSLHIPLTQETRHLVNETFIQKFK
                     KPFYLINTARGEIVSLKALKYYLDQNAILGAALDVLENEKFDALNAIEMEILKEIINS
                     RKIIITPHIAGWSHQSYVRINEVLINKIKNYLSE"
     gene            complement(3790..5160)
                     /gene="mgtE"
                     /locus_tag="VGA21_13205"
     CDS             complement(3790..5160)
                     /gene="mgtE"
                     /locus_tag="VGA21_13205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017732884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEX9651514.1"
                     /translation="MEEKVKLQFELTKEYLNGLREAIQSGNAPYIKESLEGINPVDLT
                     ELIYELNVDESKFIIDQLTPEIQAEVISELDEDVIADVLKVFSSAQIASIVSRLESDD
                     AADIIMELPVKTREEIIAEIDNKEKASNIIELLRYEENCAGGLMAKEIIKANDNWSVF
                     QCIEEIRKQAEIVHKIYSVYVVNDKGILVGRVPLKKILLSSDNTPVTEIYDEDLISVM
                     TYDSKEEAASVMRKYDLESLPVINVQGKLLGRITIDDIVDVISEQIEEERQLMSGISE
                     DVEEDDSIWMLSRARLPWLVIGLVGGILGAKVIGVFEEDLIELTAIAFFIPLIMATGG
                     NVGIQSSALVVQSLANPPAFSQSLTNKLMKTLLVALINGILLSLFVSGLVWLIDHDMK
                     LSFVVSVALFTVVLLASILGTLIPLILDKMNINPALASGPFITTFIDLIGLAIYFTVA
                     HFMYNF"
     gene            complement(5145..5921)
                     /gene="rsmA"
                     /locus_tag="VGA21_13210"
     CDS             complement(5145..5921)
                     /gene="rsmA"
                     /locus_tag="VGA21_13210"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014019094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEX9651515.1"
                     /translation="MVFAKKRFGQHFLINQGVADKIVNSLSFHNNYNTVVEVGPGTGV
                     LTARLMELKRGKLIAVELDKESIIFLKENFDVNKFTLIEGDFLKLDLAEFPSSLGIIG
                     NFPYNISSQIFFKILEHKEKVMEVVGMLQKEVARRIASAEGSKEYGILSVLVQAYYDV
                     ELLFEVSPGSFRPPPKVQSSIIRLKRNNTTNLDCDEVLFRRLVKQGFQNRRKTLWNAL
                     KPINLPDQFRSSEFMSKRAEQLSVKDFEKLTKQVEEWKKR"
     gene            6016..7032
                     /gene="pdxA"
                     /locus_tag="VGA21_13215"
     CDS             6016..7032
                     /gene="pdxA"
                     /locus_tag="VGA21_13215"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009195526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HEX9651516.1"
                     /translation="MSDKRIKPYIGITIGDLNGIGPEVIIKALSAQKILDFFNPVIYG
                     SGKVISYYRKLLDLNDFNFAQIKDEQLIPKRVNVVNCWQDNFEIKPGFGDEESGKFAF
                     LALKRACEDLKSQKIHAMVTGPINKEKMPREEFNSVGHTEFISSFFEVKETLMFLVSE
                     QTRVGLVTGHIPLKDVAMKITKSGIESKLRVMEKSLINDFGISKPRIAVLGLNPHAGE
                     NGLLGVEENNIISPVIKDFKEHGKLVFGPFPGDGFFGSGQYVQYDGVLAMYHDQGLIG
                     FKTIAFGSGVNFTAGLGVIRTSPDHGTAYSIAGKNKADESSMRAAMMLAYDIALRRFE
                     MVEN"
     gene            7107..7652
                     /locus_tag="VGA21_13220"
     CDS             7107..7652
                     /locus_tag="VGA21_13220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009583535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEX9651517.1"
                     /translation="MGRNRDYTIDIYKLSNTRHDYHFVFEDSFFGEFENSVITKGKGE
                     IDVELDKSPTFIKVDFRIIGSVELICDRSNDLYDFPFDTKGEIILKFGENTDEISDTI
                     EIIPWETQRIDLAQYIYEFIGLAVPMKKLHPRYSNDDETDGIVFSTEIEKDQTEKNEI
                     DPRWSKLGKLKNLKEKKNGAS"
     gene            7639..7824
                     /gene="rpmF"
                     /locus_tag="VGA21_13225"
     CDS             7639..7824
                     /gene="rpmF"
                     /locus_tag="VGA21_13225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011585010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEX9651518.1"
                     /translation="MAHPKRKISKTRRDKRRTHYKAYVKTYMECSNCGAPVLYHRVCS
                     ECGYYRGKQAVEVEATA"
     gene            7828..8769
                     /gene="plsX"
                     /locus_tag="VGA21_13230"
     CDS             7828..8769
                     /gene="plsX"
                     /locus_tag="VGA21_13230"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009583533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEX9651519.1"
                     /translation="MKIALDAMGGDFAPKVNIEGAKMALQELDPEIKLVLLGDEAILK
                     PLLSDLEVDPSRFEIVHTTQIIEMGEHPTKALTLKPDSGIVRAFAMLKAREIDAFCGT
                     GNTGAMHVGSMFSVKTIEGIIRPGLAGFVPKESGNYGIILDVGANADCKPDVLYQFGV
                     IGCIYAKNVLEIPNPKIGLMNLGEEEQKGSLHTQAAYQLFKINSNLNFIGNIEGRDLF
                     NDKADVIVCDGFTGNVILKLVESIFDLLKKRNFVDEYFERFNYEAIGGSPILGINGNV
                     IIGHGASTAQAVKSMILQARDMAKADIHTKIKNNLDF"
     gene            8773..9768
                     /locus_tag="VGA21_13235"
     CDS             8773..9768
                     /locus_tag="VGA21_13235"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009583532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HEX9651520.1"
                     /translation="MGKIRAAITGVGGFVPDYILTNAELETMVDTTDEWIRTRTGIRE
                     RRILKDPKAGTSYIGVNAVNQLLKKTNTKPEEIDLILCATVTPDMVFPATATLIASRI
                     GAINSFGYDIQAACSGFIFALTTGARYIESGAYKKVVIIGADKMSSIIDYTDRTTCII
                     FGDGGGAILLEPTEEEVGIIDSIHRHDGSGAPYLHIKAGGSLNPATHETVDLKQHYVY
                     QEGSTVFKFAVTNMADMAEKIMKKNNLTANDINFLVPHQANKRIIDATHERMGINEDR
                     VLMNIDRYGNTTAGTIPLLLWDFEKKFKKGDNLILSAFGGGFTWGAVYVKWAYNS"
     gene            9815..10378
                     /gene="efp"
                     /locus_tag="VGA21_13240"
     CDS             9815..10378
                     /gene="efp"
                     /locus_tag="VGA21_13240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014019059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEX9651521.1"
                     /translation="MATTADFKNGLCIEFNNNLYTIVEFQHVKPGKGPAFVRTKLKNL
                     NTGKVIDNTFTSGVKITTARIERRPHQFLYKDDLGYHFMDSNSFEQIALQQDLIDAPQ
                     FMKDGQVVEIIYHAETETPLSCELPTHVELVITYTEPGIKGDTATNASKPATLETGSQ
                     IQVPLFVEQDQKIKVDTRTGTYIERVK"
     gene            10384..10851
                     /gene="accB"
                     /locus_tag="VGA21_13245"
     CDS             10384..10851
                     /gene="accB"
                     /locus_tag="VGA21_13245"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009583530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEX9651522.1"
                     /translation="MKVKEIQDLIDFLSKTGLNEVNIETDEFKIHIVREPKVKQVVDT
                     MKNSSSASASSSVPAKAEANSDEQPKTSKASKLVEIRSPMIGTFYRSPNPDSPSFVEV
                     GDKIGPGQTVCIIEAMKLFNEIEAEISGTIVKVLVENASPVEYDQPLFLVEPN"
     gene            10877..12223
                     /gene="accC"
                     /locus_tag="VGA21_13250"
     CDS             10877..12223
                     /gene="accC"
                     /locus_tag="VGA21_13250"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015264056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEX9651523.1"
                     /translation="MFKKILIANRGEIALRIIRTCREMGIKTVAIYSMADKESLHVKF
                     ADEAICIGPAPSKESYLSMPRIIAAAEITNADAIHPGYGFLAENSEFSQICQDYKIKF
                     IGASARMIEQMGDKATAKSTMKSAGVPVIPGSEGLLSSVDEAKEIAKGIKYPVILKAT
                     AGGGGRGMRIVNSEEQMQKAWDDARRESGAAFGNDSLYLEKFVEEPRHIEIQIVGDKN
                     GKACHLSERDCSIQRRHQKLVEETPSPVVNQTLREKMGQAAIKGAEAIGYEGAGTVEF
                     LVDKHGDFYFMEMNTRIQVEHPITEEVTDYDLIKEQIKVAAGITISGKNYYPNLCSME
                     CRINAEDPAKDFRPNPGKITNFHMPGGHGVRVDSHAYAGYVVPPNYDSLIAKLIVSAQ
                     TREEVIVRMKRALSEFVIEGIKTTIPFHLKLMDDKNFQSGNFTTKFLESFDFSDLG"
     gene            12315..14108
                     /locus_tag="VGA21_13255"
     CDS             12315..14108
                     /locus_tag="VGA21_13255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEX9651524.1"
                     /translation="MKVWIIISSFIFIGQSPGYQGKVDSLTLIASQTTVDTVKIDALY
                     EIAKIFYGQNSKDFDVFAGELLVLAEEVNYSRGIARILYLTGLYLHREGKFIEALQLM
                     KRLQEIGSQPGLTFYKGQALALTGIIHKERGRLNQGLEYLEEAAKIYKAIDDKNRLIN
                     VMNATAGIYTDLAKIHSNDSNYLEIGYMKLMEVNSMAKELGHLTMIANSNALLGDIFR
                     MKKNFAKSISLYKEAWFIADSIGNKNMSTDIWGVIGFSFYESGQIDSARIYLTQAIAA
                     SRMYNYEPLSIKYSIELGRTYISTGDYAVAQNILTQTEKQARSLGFWNYLNDIIKMKV
                     EIFSQLKDFENGLSAMSELDIVTDTLNKIASRDKIALLESSYELDNAELKLAQMEEEK
                     EYQAKIKKIVIGGSIPLAALAIITIVLLWRWNLGKKKMLATQQNLHHLEISNLQLQEK
                     NLKMNLELKNKELAAQALNLVTKENLLKDIRESVTQLNDGVQSPAAIHAILKQIELSQ
                     NRDKDWNEFKLYFEQVHVDFFKELNSRIGDLSPSEIKLSALLRLNLSIKQCADILGIS
                     PESVKMARHRLRKKLQLQTEDNLITFLMTLN"
     gene            complement(14109..15515)
                     /locus_tag="VGA21_13260"
     CDS             complement(14109..15515)
                     /locus_tag="VGA21_13260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012930694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEX9651525.1"
                     /translation="MNSNSKLISFFRLVRQAIRGEEENFTEGSINRAIFLLSLPMVLE
                     MAMESLFAVVDIFYVSRLNNTDAVSAIGLTESILTIIYSIAIGLSMGATAMVARRIGE
                     KNKEGAAIAAIQSLYIGFAAALIFSVAGFFFHRDLLTMMGATEGILSTGANYAKWMLI
                     GNFSVMLLFLFNAIYRGTGNAAMAMRALWLSNILNIILDPIFIFGLGPIPSFGVEGAA
                     IATNIGRTIGVAYQVYYMFSGKGILKVNRSHLVVRVDIIKRLLKVSAGGTAQFLIGSA
                     SWIFLVKIMANFGSAALAGYTIAIRVIIFTILPSWGLANAAATLVGQNLGAGQPDRAE
                     KSVWRTGYYNMAFLGLVMIVFLVSSRPILNFFTGDKEVIDYGTNCLQIIALGYLFYGY
                     GMVITQSFNGAGDTRTPTILNLIFFWLVQIPLAYLLSSTFGFGANGVFSAVPIAETAL
                     AITGIIIFKKGKWKEMKI"
     gene            complement(15703..16296)
                     /locus_tag="VGA21_13265"
     CDS             complement(15703..16296)
                     /locus_tag="VGA21_13265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017734038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3109 family protein"
                     /protein_id="HEX9651526.1"
                     /translation="MISHKGTLISEDIIENYFVCDLIKCKGACCVEGDLGAPLEDDEL
                     DLLKREYPKIKPFLAKAGIKEIEKQGEYVLDSDGDYSTPTIKGKECAYSVYDERGILK
                     CGIEQAFLAGKSKFRKPISCHLYPIRITKYKEYEAINYNRWHICGPACSNGEALKVPL
                     YVFLKDALIRRYGKKWYEELIALIQQSEPEKVSSKSK"
     gene            complement(16301..17080)
                     /locus_tag="VGA21_13270"
     CDS             complement(16301..17080)
                     /locus_tag="VGA21_13270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651527.1"
                     /translation="MSNSEFYPLIAPKKDGLLALESDSWILAPIKTLGNYLEVYSLLL
                     STAYNHRIYLDLSAGTGMLEMNNTGECIYDTSLLAFSSPVQFNRYIFWEPNETYAQAL
                     RVRIKRNFKDKNVLIISTSWEKFLEQVRNYVPINTKGIKPWIFCNVNPRFGFPEIHFF
                     DSIESLDPVVFFTLAGEKKKFWTKLKSGKDDFELYARNHIRNIEKWTYENNFRIKGSF
                     SLTESSKMNSLYYQGVLCRKANVSKVVTEVRRRSSNQIQLF"
     gene            17209..19464
                     /locus_tag="VGA21_13275"
     CDS             17209..19464
                     /locus_tag="VGA21_13275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017734037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEX9651528.1"
                     /translation="MEYLHSLNEAQKEGVINTEGPCMVIAGAGSGKTRVLTYRIAHLI
                     KSKGVDPFNILSLTFTNKAAGEMRNRIESVAGLDARNLWMGTFHSVFARILRSEGTRL
                     GYPNNFTIYDTDDSKSLIKSIVKEMGLDEKLYKPGIVYNRISGAKNRLIGWEDYQNNP
                     LYLADDESTLRPEIANIYRNYCLRCFKAGAMDFDDLLLKTNELFEKFIEVLNKYQHRF
                     QYVLVDEFQDTNLSQYKITRKLSSVNQNICVVGDDAQSIYAFRGADIQNILNFEKDFP
                     DLRIIKLEQNYRSTQNIVNAANSVINNNRAQLKKNVWTTNPEGSLIELFKANSDNEEG
                     RLVASSVFEEKMQNQLHNSDFAILYRTNSQSRAMEEALRRMNIKYKIIGGLSFYQRRE
                     IKDLLAYLRFSVNHNDEESLKRIINLPKRGIGDGTISKIMVAAEEHAIPVWDVLCNIK
                     SFMSGRAANAIEDFTILIKSFEIEIGKKDAYEVASHIAKASGILKELYQDKTIEGLNR
                     FENVQELLNAVKEFVDNQENEEKNLNTFLQEVALLTGIDNDKDEDNDKVTLMTIHMAK
                     GLEFKNVYIVGLEEDLFPSQMMLTSRADLEEERRLFYVAITRAKEKLFLSYAVTRYRF
                     GRLKSCEPSRFLDEVDPQYIKENRKFNTVQNYSTSPATNYARNFVNNIRKTSSPKQTP
                     ASYQTPIDFEASDTTRLKAGMKVEHPKFGFGKVISMEENGINRKAKVHFDGIGQKTLL
                     LSFAKLKIHEE"
     gene            19520..20170
                     /locus_tag="VGA21_13280"
     CDS             19520..20170
                     /locus_tag="VGA21_13280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013452724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4290 domain-containing protein"
                     /protein_id="HEX9651529.1"
                     /translation="MEKIFEYNTERKNLILKEYGRNIQKLVHYLKTIEDKEKRSQMAA
                     TLIELMKQISPSIRENNETNQKLWDDLYIMSGFDLEIDSPYPMPPLDILNKKPLRLGY
                     STNNIRFKHYGKNLELLIAKAMELEDPEEREGAVVYIGKLMKSFYSTWNKDNIEEESI
                     LKNIRELSKNELDIDITKVKENNLFESLYKERPKPPPQHTNRGRKNQRGGGSKRRR"
     gene            20173..21480
                     /gene="murA"
                     /locus_tag="VGA21_13285"
     CDS             20173..21480
                     /gene="murA"
                     /locus_tag="VGA21_13285"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019599329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEX9651530.1"
                     /translation="MSSFRIVGGNQLKGEIIPQGAKNEALQIISATLLTPEKVTIHNI
                     PEIRDVQRLIQLLKSLGAQVEDKGKGTFTFAASNIDLGFLDSPEYLEMGSTLRGSIMI
                     MGPLLARFGKGKIPRPGGDKIGRRRLDTHFIGFQKLGAKFEYDPGTGFYTVEANHLKG
                     TYMLLDEASVTGTANLIMTSVLAEGKTTIFNAACEPYIQQLCKMLVRMGANIDGIGSN
                     LIMIEGVNKLKGTEHTLLPDMIEIGSFIGLAALTQSEITIKNAGVNHLGLIPEVFRRM
                     GIQMLIDGDDIFIPAQSEYVIDTFIDGSILTISDAPWPGFTPDLLSIALVVATQARGT
                     VLIHQKMFESRLFFVDKLIDMGAQIILCDPHRATVIGLDRKYPLRGINMSSPDIRAGV
                     SLLIAALSAEGTSIIYNGEQIDRGYQNIDGRLRGLGADIQKVD"
     gene            complement(21491..22615)
                     /gene="dnaN"
                     /locus_tag="VGA21_13290"
     CDS             complement(21491..22615)
                     /gene="dnaN"
                     /locus_tag="VGA21_13290"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010611734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEX9651531.1"
                     /translation="MKFIVSSSALLKQLSGINGVITTNPVVPILENFLFEINDSKLTI
                     TASDLQTSMITELEIESKESGSIAVPAKILMDTLKNLPEQPVNFSIDEQTYSVEISSD
                     NGRYKLSGENATDFPKVPSVSDDFSVEMSSGVLAKAVASTIFATSNDELRPAMTGVYV
                     NLSDSNTTFVATDGHRLIRYRRADVKSENGSAIIIPRKALNLLKTTLPVDEVPVNIEF
                     NMANAFFRFGNIKMICRLIDERFPDYENVIPKDNKNKMVIDRAELLSSMRRIAIYANK
                     TTHQVRLKITGSELQISAEDLDFSNEANERLSCEHDGEDIEIGFNARFLIEMLSNGDA
                     KEVEFQFSAPNKAGLIVPREKEDNEDILMLVMPVMLNNYV"
     gene            complement(22707..23654)
                     /locus_tag="VGA21_13295"
     CDS             complement(22707..23654)
                     /locus_tag="VGA21_13295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651532.1"
                     /translation="MQILSRRIHNGYWIASLVILSGLTFFVYTSGERSGENFDENKFT
                     LEVNSIITSVDISSPGVNLNFEFLEGEWIVNDSFRMDPGMRDAFFTVISSIKVKKPAG
                     INQKDSLINFLKTSGSKVRIINNQNLIKSYLVGGDELSGVSYFMDSDDEAPYEVILPG
                     YKSYVAGIYSVPLKDWRNRFLFELDWSSLKSILVEYINNESPGFSVNYKEDFFYLNNN
                     SQPIDSAVIFDYIGELMNLQAERFLLPEEDLAYDSLAASLPLAKVVLVTIGSNDQVLE
                     VFPTIKERRLTLARYNGELLVLFNPRSLGPVLIHNEHWE"
     gene            complement(23638..25317)
                     /gene="gldG"
                     /locus_tag="VGA21_13300"
     CDS             complement(23638..25317)
                     /gene="gldG"
                     /locus_tag="VGA21_13300"
                     /inference="COORDINATES: protein motif:HMM:TIGR03521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     substrate-binding protein GldG"
                     /protein_id="HEX9651533.1"
                     /translation="MNSRKVGDILKFLNLAVMLLLLNIMANRNFFRLDLTEEKRYSIS
                     TATKEILTSLDDNILVEVYLGGELPPAFERMQKSIREILEEFRIYSDYKVSFRFIDPE
                     QAGNAQSRQNFFQSISEKGILPYDVFMNKDGNRIQKRILPGAVVSYGLAEKGVQLFKG
                     NQSAAPEVRLNQSIEGIEYEIASTIKTITNDMVPAISFITGHGELDSINIISFRSTLS
                     ERYRVQSIDLPETIEIPNTDLIIIIKPVRAFSDQDKFKIDQYIMRGGRAMFLLDMLNV
                     KMDSIQTGTFAFGYDLALQDLLFKYGVRLNQDLIQDVISGSQPIVIGSMGDQPQIQLM
                     PWPFNPILNNFSAHLITRNLDAIYGRFVSSIDTVKATGVAKIPLLYTSAYTRRLSAPV
                     RVSAEDLKIQLTPDQMNEKNIPVAYLLEGTFTSLYKNRFPPPGIDRDQEITGESGNTG
                     IIVITDGDIARNEINSSTGRPMELGYDPYSRQMFGNMDFLLNAVDYLVDGKGLITARA
                     KEVQIRPLDKVKIGEQKGFWQTLNLVTPLVLLFAYGIIRFYLRKRRYANFK"
     gene            complement(25314..26048)
                     /gene="gldF"
                     /locus_tag="VGA21_13305"
     CDS             complement(25314..26048)
                     /gene="gldF"
                     /locus_tag="VGA21_13305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013453568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     permease subunit GldF"
                     /protein_id="HEX9651534.1"
                     /translation="MLHIYSKEINSFLNSLIGYIVISVFLTGLGLLVWIFPETSVLDY
                     GYADLETLFSFSPYIFIFLVPAITMRMFAEEKKSGTLELILTKPVSGMSIIIGKFLSG
                     LTLVIVALLPTGVYYYSMHSLGNPAGNIDSAEVMGSYLGLILLGGVFVSFGIFASSIT
                     RNQIVAFILGVFFCFVMYQGFSSISIMPGVEGNSLLIEQLGISYHYSSMSKGLIDSRD
                     AVYFLTIIIIMIGSTNLVLKSKQWGS"
     gene            complement(26051..26968)
                     /gene="gldA"
                     /locus_tag="VGA21_13310"
     CDS             complement(26051..26968)
                     /gene="gldA"
                     /locus_tag="VGA21_13310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009194724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     ATP-binding subunit GldA"
                     /protein_id="HEX9651535.1"
                     /translation="MSIRIEGLTKIYGKQKAVDGISFEVNQGEIMGFLGPNGAGKSTT
                     LKMITGYLPPTSGNISVCNLDVQESGAEVRKIIGYLPEHNPLYLDMYVHEFLQFVGKI
                     HGFRGNELIKRVEEIIILCGLAEEENKLLDSLSRGYRQRVGLAQALFHDPEVLILDEP
                     TTGLDPNQILEIRKLIKEVSSRKTVLFSTHIMQEVQALCSNVVIINKGKIVANDTIDV
                     LTRMKGKETRIELEVIPEIDMARLAEIEGVNNVISKGNGHYEITASFEKDLRPLISRK
                     VMELGSDVVGLKLFESSIESIFQELTRQE"
     gene            complement(27015..27713)
                     /locus_tag="VGA21_13315"
     CDS             complement(27015..27713)
                     /locus_tag="VGA21_13315"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008203134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9651536.1"
                     /translation="MKIENKIAIVTGASKGIGLETCKLLISKGVRVAGWSRTAPGLEH
                     KNFQFFAVDVSNLKSVENGYAATIKGFGVEVDILINNAGLGYEYPFEETPLDLWQKMF
                     STNVDGIFYCSRLVIPGMKKKEEGHIINISSIAGTNGIVNMSAYCATKHAVRGLSQSM
                     FKELRNYGIKVTCIYPGSTKTNFFDEISSVKISDNMMMPEDIAGTIIQCLETSGNYHP
                     VDIEVRPLRPKGKQ"
     gene            complement(27715..28518)
                     /locus_tag="VGA21_13320"
     CDS             complement(27715..28518)
                     /locus_tag="VGA21_13320"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX9651537.1"
                     /translation="MNRVIYIIFLISFFLLTTSGLVCSQNKELPDSVKLNGKYIRGYF
                     TNTWAMIKSPANWEEKDWRNFGMTGAATFVLYIFDEDIVDWVSEIRSNESNTVSDIFE
                     PFGNSSVLMPFSVLFGTYGIGVLTKDYRLKKSSLVALQSFAISGFFTQAIKFAVNRDR
                     PFRGHRAFPSGHTSAVWSVAAVFAYEYKDKMWVAPVAYSLATLTSLSRINDLRHWPTD
                     VVFGFALGYFTGRYISRIHDRNVEKNLNLSLSPFYGNEFKGLSLVYRFR"
     gene            complement(28511..29491)
                     /locus_tag="VGA21_13325"
     CDS             complement(28511..29491)
                     /locus_tag="VGA21_13325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651538.1"
                     /translation="MKGKKTVGIYIKIVAGIFIVLPIIVGSGQSFAQGQVNWDWWCTN
                     TGQAIGCPTDNWVDYISISPGGMGPNALPVPSVAKGRVDNELSLELGAQAHFGAGDNT
                     QNLWTYLNVNLAKDIASLVIYAIPQEHFDVSDQTRDERMLSGEYFEPEGFAKGDVYFG
                     MEIQILKDRPRGPDLLLRAVTKTASGSETGGARFTDSPGYFIDVSAGKDFQLGEENKW
                     LRPYANLGFYAWQTYDRKHPQNDAPMYGLGLDLILNKNSFSTSLSGYAGWKDNGDKPL
                     VYRLEYTRKAKTFNYLAQYQLGFNDFDYTSLKLSIIYHTENLFRKKKNSE"
     gene            complement(29493..30326)
                     /locus_tag="VGA21_13330"
     CDS             complement(29493..30326)
                     /locus_tag="VGA21_13330"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020532804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEX9651539.1"
                     /translation="MNSGMIPSKYFEALDFIKKRISDSPEIGIILGTGLGDLAKEIDI
                     KSEISYSDIPHFQVSTVEFHEGRLLFGTLGNKQVVAMNGRFHYYEGYNMEQVTFPVRI
                     MKLLGITHLFISNASGGLNSAQEISDLLIMKDHINLFPDSPLRGEHNEEFGPRFPDMS
                     EPYNRDLIAKAVDIAKRLAIRAHVGVYAGVAGPALETPAEYEYIRRLGADAVGMSTIP
                     ENIVARQMGIDCFAISVITDLGIEGKIKKVTVEEVIAAAKTAEPGMTKIMKELIQGIQ
                     A"
     gene            complement(30450..30917)
                     /locus_tag="VGA21_13335"
     CDS             complement(30450..30917)
                     /locus_tag="VGA21_13335"
                     /inference="COORDINATES: protein motif:HMM:NF025227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HEX9651540.1"
                     /translation="MQDTKYSIESISVRFGLLTTVGLLAYFLIMKFVGLVHIVELRTL
                     NFLVLVIGIYKALKAFQKNNEQPMGYLQGWGIGCLTSSVAVLPFALFIFIYLQLDPAL
                     MEYIRNRDEFGRFLNPYMLAFVIAFEGIVSGLVLTFILLQWMMKSHLKGGGLR"
     gene            complement(31417..31830)
                     /locus_tag="VGA21_13340"
     CDS             complement(31417..31830)
                     /locus_tag="VGA21_13340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019942140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEX9651541.1"
                     /translation="MIKIFVDTDVCIDLLSERMPFNIHAAKLFSLADNHKIKIFVSSI
                     SFANIDYVLRSQFSGKSSRQMLAKFKTLVTVLPFDSKTIDLSIASDFADFEDAIQYNT
                     AIENGQSILITRNTKDYKKAHIQVLTPETFLTSRL"
     gene            complement(31833..32081)
                     /locus_tag="VGA21_13345"
     CDS             complement(31833..32081)
                     /locus_tag="VGA21_13345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009580846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6364 family protein"
                     /protein_id="HEX9651542.1"
                     /translation="MDTKLTLKLDDNIIEKAKVYAKKRKTSLSKLIETYLDYLTSGNM
                     EAGEITPLVKSLSGVLNLPKNYDYMKSYKDHILKKYSK"
     gene            32627..>34055
                     /locus_tag="VGA21_13350"
     CDS             32627..>34055
                     /locus_tag="VGA21_13350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651543.1"
                     /translation="MKTINQSKGLLIFIVLLTVIFQKASCQISSFPYLEDFEADDGGW
                     IAQNNGAPNGWEYGTPTGFSLNSAHSGIKVWGTNLDGDYDNNANLYLESPEFDFSGMV
                     TDPYFLISTAFIIENYYDGAYIEYSTDMGVIWTVLGDLTAGQNWYNKTLSNGEGWSGS
                     YPDWSVASIRLSGLADQPSVMLRIVFTSDESITREGILIDDVQIVSNFTDMKISAEEN
                     NILLTSEQSTINVDNIGTDISTPFSIQVEITRNLEVLHQETFQNITLTEGQSLALNPA
                     YYFDEPGGYTLTATAQISDFIESNNGFTKKLIVIGSSGSTLPYTAGFESGDADWVTED
                     QVVNSWELGYPAGNVLNAPHTGLNSFVTNLDGNYDNNAVLELYSPLFDFSSANGSESF
                     QFAINHELEPGYDFAWVEFSTDLGESWSKLGTNLSGGNNWYNFVQGWSENSAGWKTAS
                     HSLSFFSGEATVILKIVVSSDGIAVK"
BASE COUNT        10543 a         6814 c         6753 g         9945 t
ORIGIN      
        1 tcggttgaat gatcatctac aattattact tcaaaatttt tgaccgggta atcctgcagg
       61 ttaatagatt ctataatttt agtaatattt tcttcctcat ttcgacatgc aattattacg
      121 gaaatgaaat cattatattc gtcaaccttt ttattgaatt ttattcttct ccaattaaaa
      181 agcagaataa taagacatag tgaatgcagg acaaagtaac taatgacaaa tatattccac
      241 atggttgcta aagcagacta atggattaaa gaattgatta agaccttaaa atgcaataaa
      301 atgattaatt tgaatcagat aagatgacaa aagttaataa taatgaaaaa attattgttg
      361 gattagatcc aggaaccagg ataatgggtt acgccgcgct gttggttcag aattccaggt
      421 tgaaattgct gcaatatgga gttatcagac ttgaaaaata ttcagggtat gaattgaagc
      481 ttaaaaaaat atttgaaaga gttacaggtc ttatagaaga attttctccg gatgagattg
      541 cacttgaagc tcctttttac gggaagaatg tgcaatcgat gttaaaatta gggcgtgccc
      601 agggcgttgc aatggcagct gcccttcatc atcaaatccc cattgcagaa tatgcaccta
      661 aaaaagtgaa gcaatctgta accggaaacg gaaatgcatc caaagaacag gtggccaaaa
      721 tgatgcaaac tcttttaaac attaaggaaa cacccaagct tttggatgca acggatgcac
      781 tggcagtagc catcactcat tattttcagg ccaggccaaa gaaaggaact tcgaaaagct
      841 ggaaagcatt tatagctgat aacccgggaa ggataaaaag ttgacgggca agttttaatt
      901 taaatttgat ttaatttttg aagctatctc tgcaagttca tcatcagtag gtttcaattt
      961 cggccgcgta aaattaatgt cattcataga attcaatgga atcaggtgaa tatggcaatg
     1021 cggaacttca attcctataa ccgccacacc gatacgcaaa catggtaccg cttctctaat
     1081 tgcagaggaa actttctttg caaattccat taagcctgac agagtctcac tatcaagatc
     1141 aaatatataa tcgacttctt ttttggggat caccaataca tgccccataa ccaggggatt
     1201 tatatcaaga aatgccagaa aatgattagt ctccgccaca atgtgggctg gaatttcgcg
     1261 atttacaatt tttgtgaaaa tggttgccaa gtctcaggaa atatctaaaa ttttgaaatt
     1321 tattttgcct gccggaacct gaacacttgc tgactgcccc acctttttcc caagcaatcc
     1381 tttgccgatt ggtgaatcaa cagaaatctt tccttcattc aagtcagctt cttcttcagc
     1441 cactatggta taggtaaatt ttgaaccgtt gtttttattt tgaattgtca cacttgataa
     1501 tatcgatacc ttagacttgt caacggtgga ttggtcaagg attcgggcat ttcccaggat
     1561 ggtctccaat ttggatattt ttaattcaag caatccctgg gcatctttcg cggcatcgta
     1621 ctcagcattt tcgctcagat ctcctttatc ccttgcgtcg gctatctgtt tagcaatttc
     1681 actccgacct ttagttttca attccgaaag ttcctctttc agttttttaa gtccttcttc
     1741 ggtgtaatat gatatctttc ccatgattgt attttgataa gtccttataa aaaaaaatac
     1801 ggcctcaatg aaaccgttat catttgattg ttagttattt attaagctaa tttaagttat
     1861 aaaagtataa caggcaagca cttgaggcta attaacctac tttcataggt cgttcaatgc
     1921 caagtaattc agggaattgg agttccatct caatagcaat ttcaggatcc agtttagcct
     1981 ggtaaagagt tttggccttt gaaagctgtt ccacagtaat tccggttgcc gaccttaggt
     2041 ctgctttata caaacttgca ttttccagat ttgcaccggt taaatgtgta cccgaaaggt
     2101 ctgcttccat caggaaggca tttacaaaat tggcttttat aagaaagctg ttacgtaagt
     2161 tggtttttat caggaaggca tctttaaaat tcgcaccacc tgcataagaa tccgaaaggt
     2221 ctgcctggtt aagattcgcg ttttcgaaac aagtttgatt tagccgggtt tttttaagat
     2281 tagcctggat aagaaatgag tttcggatat tggcagaatt aaggttggat tcttccaggt
     2341 caatatggtt gaggttggtt ctggccaggt atgtttcaac caaatcaatc ctgaaaatgt
     2401 tgtggcgatt caatctttta atatttccga cggttctaaa cgcggcttcc tcagattccc
     2461 ataatctaaa gtcatcaatt tcatctctgt acgttctgat gcgctgtcga acttcccccc
     2521 tacggttcaa ccagaaaatc aaaataccaa tcaccgcaat gtcgaaaatc attccgtgtg
     2581 cttcagccaa aatttgggca tagaagtttt taaaatctcc cagataataa ggaagactca
     2641 tgccgattac caaaatggca gttaaaagca aaactataaa cgaggtgagt attggctttt
     2701 ctgaaatttt tgataaggtt tcataaagtc gaaagaattg ctttgccatt gccagatcga
     2761 attacagccg aaaagtataa caattaatta atataattca atattatacc atctggctgc
     2821 tgataagtaa tggaataaca ggttattctg atagataatt cttgatttta tttataagaa
     2881 cttcattaat tcttacatag gactggtgcg accagccggc aatatgagga gtgattataa
     2941 ttttccttga attaatgatt tcctttaaaa tctccatttc aatggcattt aaggcatcaa
     3001 atttctcatt ttccaataca tcgagcgccg ctcccaggat tgcattttga tccaaataat
     3061 acttcaatgc tttcaatgaa acaatttcgc ctctggcagt attaattaag tagaaaggtt
     3121 tcttgaattt ttgaatgaaa gtctcattga ccagatgtct ggtttcctgg gttaggggta
     3181 tatgcaagct gagcacatct gttttattaa aaatagtctt aagcggaact aaacggacga
     3241 agtcttcagc aatctcattc ttgtatttat cataagcaat aatctccact ccgaatgatt
     3301 tgagtctgtg agccaccgcc tgtcccatgt ttccatagcc aaatatcgca acggtttttc
     3361 ccattaactc ttctcccctg tttgcttccc tgttccattg caaagatttt acttcatggc
     3421 tggccagatg gatcttatta agcagggaaa ggagaagtcc tacagtgtgt tcggcaagcg
     3481 tatcccggtt tccttccggc gcattaatga tttttatacc tctggtctca agttcatcag
     3541 tatctaaatt atccgttccg gctccggccc tggcaacaaa ttttaaattt ggagcctggt
     3601 ctaaaaaggt tttatcaacg ctgactttac ttcgaacaat taatccttca taagttccga
     3661 taatttgtaa aatttctgat tggttaattt cagggaaata gtcaacttct aaccctagtc
     3721 cttccagggc tggggcaata ctaggatgca ttttatcaat gatgatacac ttcacaggaa
     3781 tccagatttc taaaaattat acataaagtg tgcaacagta aagtatatcg ccaatcctat
     3841 caggtcaatg aacgttgtaa tgaaaggtcc ggatgcaaga gcgggattaa tattcatttt
     3901 atccaatatt agcgggataa gtgttcccag tatagaagcc agtagtacaa ctgtgaaaag
     3961 agcaacgctc actacaaagg aaagcttcat atcatggtct atcagccaaa ccagacctga
     4021 tacaaaaaga cttagcagga ttccattgat gagagctaca agcagggtct tcatgagctt
     4081 atttgtcaga ctctgcgaaa aggcaggtgg atttgccagg ctttgtacaa ccagggcaga
     4141 ggattggatt ccaacgtttc ccccggtggc cattatcaat ggaatgaaaa aagcgatggc
     4201 cgtgagttca attaaatctt cctcaaagac accaattact ttcgctccaa gaattcctcc
     4261 aaccaggcca attaccagcc agggtagccg cgccctggag agcatccaaa tgctgtcatc
     4321 ttcttccaca tcttcggaaa taccggacat taactgccgc tcttcttcga tttgttcaga
     4381 aattacatca acaatatcat caatagtaat tctacccaac aactttccct ggacgttaat
     4441 tacgggcagt gattcaagat cgtacttcct cattaccgac gcagcttcct ctttgctgtc
     4501 gtaagtcatt acagaaataa ggtcctcatc ataaatttcg gtcaccggtg tattgtcgct
     4561 tgacaataat atttttttga ggggcactct tcctacaaga atgcctttat cattaaccac
     4621 atagactgaa taaattttat gaacaatttc ggcttgtttc cggatctctt caatacattg
     4681 aaatactgac cagttatcat tggctttaat tatttctttg gccatcaatc cgccggcaca
     4741 attttcttcg taccgcaaaa gctcaattat attcgatgcc ttttctttat tatcaatttc
     4801 cgctattatt tcctcccggg tctttacagg aagttccata atgatatcgg cagcatcatc
     4861 actttcaaga cgcgaaacaa ttgaagcgat ttgggcggag ctgaatactt tcagaacatc
     4921 tgcaattaca tcttcatcaa gctcactgat aacttcggcc tgaatttccg gggttagttg
     4981 atcaataatg aactttgatt catcaacatt gagttcatag attagttcag taagatctac
     5041 cggatttatt ccttccagag actccttaat atatggagca tttccggact ggatagcttc
     5101 tctaagccca ttcagatatt ctttggtaag ttcaaattga agttttacct tttcttccat
     5161 tcctcaacct gtttggttag tttctcaaaa tccttgacac tcagctgttc agcccgttta
     5221 ctcataaact cagaagacct aaactgatcg ggcaaattta ttggttttag tgcgttccac
     5281 aaggtcttac gccggttttg aaacccttgc ttaaccaacc ggcggaacaa aacttcatca
     5341 caatcaagat ttgtagtatt gtttcgcttt aaccgtatta tcgaagattg cactttcggc
     5401 ggtggacgga atgatccggg cgacacctca aagagcagtt caacatcata ataagcctga
     5461 acaagaacac tgagaattcc atattccttt gagccttccg cggaagcaat tcgtctcgca
     5521 acttcctttt gcaacatccc aactacttcc attacttttt ctttgtgttc caggattttg
     5581 aaaaaaattt gcgatgaaat gttataagga aaattaccaa ttatgcctag tgaggatgga
     5641 aattctgcca gatccagttt taagaaatct ccttcaatca aggtgaactt atttacatca
     5701 aaattttcct taagaaaaat aatggattct ttgtccagtt caacagcaat taattttcct
     5761 ctcttcaatt ccattaatct ggcggtcaac acgccggttc caggtcctac ttccaccaca
     5821 gtgttatagt tattgtgaaa agagagcgag ttcacaattt tgtctgccac tccctggtta
     5881 attagaaaat gttgtccgaa tctcttcttt gcaaaaacca taatataccg gtaaatctaa
     5941 gtattgctaa gcctccaccc cgccgttttg aaattaatta tcaaatttga attaacttgg
     6001 ttataaaaaa agataatgag cgacaagagg atcaaaccat atatcggaat tacaattggt
     6061 gatttaaatg gaataggacc tgaagtcatt atcaaagcgt taagtgccca aaaaattctt
     6121 gattttttta acccggtaat ttatggctcc ggaaaagtaa tttcctatta cagaaaatta
     6181 ttggatttga atgattttaa ctttgcacag ataaaagatg aacaattaat tcctaaaaga
     6241 gtaaatgttg ttaattgctg gcaagataat ttcgaaatca agccaggttt tggagatgaa
     6301 gaaagcggga aattcgcgtt cctggcatta aaaagagcct gtgaagatct gaagagtcag
     6361 aaaattcatg caatggttac aggtccgata aataaggaaa aaatgcccag ggaagaattt
     6421 aattccgtgg gccacactga atttatcagt agtttctttg aggttaaaga aacgctgatg
     6481 ttcctggtta gtgaacaaac aagagtagga ctggtaaccg gtcatattcc tcttaaagac
     6541 gttgccatga agattacaaa atctggtatt gagtcgaaat tgagggtaat ggagaagtct
     6601 ttgatcaatg attttggaat ttcgaaaccc agaatcgcag ttttaggctt aaatccgcac
     6661 gcgggtgaga atggtttgct cggagttgag gagaacaaca taatttctcc ggtaataaaa
     6721 gattttaaag aacacggcaa gttggtattt ggaccctttc ctggagatgg tttttttggc
     6781 tcaggtcaat atgttcaata tgatggagtt ttagccatgt accacgacca ggggttaatt
     6841 ggatttaaaa ccattgcctt tgggagtgga gtaaacttta cagctggact cggtgtaatt
     6901 agaacctctc ctgaccatgg aaccgcttac tcaatagccg gcaaaaacaa ggccgatgag
     6961 agctcaatgc gggctgccat gatgctcgcc tatgacatcg ccctaagaag gtttgaaatg
     7021 gttgagaact aggccataat tacctcttta cttggtattt atttgaatta tctttttaaa
     7081 tttgcgcctt atttggcttt tgggaattgg gaagaaaccg ggactatacg attgatattt
     7141 ataaattaag caatacccgg catgattatc attttgtttt cgaagattcg ttttttggag
     7201 agtttgaaaa tagcgtgata acaaagggta agggtgaaat tgatgttgaa cttgacaaat
     7261 cacccacgtt cataaaggtg gatttcagga tcataggttc agttgagcta atatgcgatc
     7321 gttccaatga cctatatgat ttcccgtttg acaccaaagg ggagattatt ttaaagtttg
     7381 gtgagaatac ggatgaaata agtgacacca tagaaataat tccctgggaa actcagcgga
     7441 ttgatttggc tcagtacatt tatgaattta ttggacttgc cgtaccgatg aaaaaattac
     7501 atccccggta ctctaatgac gatgaaacgg atggtattgt gttttcaaca gaaattgaaa
     7561 aagaccaaac cgaaaagaat gaaattgacc caaggtggtc aaaattaggt aaactgaaga
     7621 atttaaaaga aaagaaaaat ggcgcatcct aaacgcaaaa tttcaaaaac gcgaagagac
     7681 aaaagaagaa cccattataa agcatatgta aagacctaca tggaatgctc caattgtgga
     7741 gcaccggtct tatatcacag agtgtgctca gaatgcggat attatagggg caagcaagct
     7801 gttgaagttg aagcaacagc ctaattaatg aagattgctc tcgacgccat gggcggtgac
     7861 tttgccccaa aagtcaatat tgaaggggcc aaaatggcac tgcaggaact tgaccctgaa
     7921 attaaactgg tcctgttggg tgatgaagca attttgaaac ccttgttgtc cgatcttgaa
     7981 gtagaccctt cacgatttga aattgttcat acaacccaga ttattgagat gggtgaacat
     8041 cccacaaagg ctttaaccct aaaaccagat tcaggcattg taagggcctt tgctatgcta
     8101 aaagcgcgcg agattgatgc attttgcggt acaggtaaca ccggcgcgat gcatgttggt
     8161 tccatgtttt ctgttaagac aattgaagga ataatcaggc caggtttggc aggatttgtt
     8221 ccgaaggaat caggaaatta tggaattatt ctggatgttg gagccaacgc agattgcaag
     8281 cctgatgttt tgtatcaatt tggtgtaatt gggtgcatat atgcaaagaa tgtacttgaa
     8341 attccaaatc caaagattgg attaatgaat ctgggggagg aagaacaaaa agggtcactg
     8401 catactcagg cagcctatca gcttttcaaa ataaactcaa atctcaattt tattggtaat
     8461 attgaaggca gagatctatt taatgataag gccgacgtga ttgtttgtga tggatttacc
     8521 ggaaatgtta tactaaaact cgttgaatca atttttgatt tactgaaaaa gcgcaatttc
     8581 gtcgatgaat actttgaaag attcaactat gaagctattg gcggaagccc tatcttgggc
     8641 attaatggta acgtcataat cgggcatggt gcttctaccg cccaggcagt taagagcatg
     8701 attttgcagg ctcgagacat ggcaaaagca gacattcaca cgaaaataaa aaacaacctg
     8761 gatttctaag caatgggaaa aataagagca gcaataacag gagttggtgg atttgttccg
     8821 gattatatac tcactaatgc tgagcttgaa accatggtgg ataccactga cgaatggata
     8881 agaaccagaa ccggaatcag ggagagaaga atactgaagg atcctaaggc cggcacttct
     8941 tatataggag taaacgcggt taaccagtta ttgaaaaaaa caaataccaa accggaagaa
     9001 attgatctca tcttatgtgc taccgttact cctgatatgg ttttccccgc cacggcaact
     9061 ttaattgctt cccggattgg agccataaat tcatttggtt atgatataca agccgcctgt
     9121 tccggattta ttttcgcatt aacaaccgga gccaggtata ttgagtcagg tgcgtataaa
     9181 aaagtggtga taatcggtgc tgataaaatg tctagtataa ttgactacac cgatagaacc
     9241 acttgcataa ttttcggtga tggcggcggt gcaatcctgt tggaaccaac agaagaagaa
     9301 gtaggaatta ttgattctat ccaccggcat gatggtagtg gtgcacccta cctgcacatt
     9361 aaggcaggtg gaagtttaaa ccccgcaacc catgaaacag tagatttgaa acagcattat
     9421 gtttaccagg agggttcaac agtgttcaaa ttcgcggtga ccaatatggc tgatatggca
     9481 gaaaaaatca tgaagaaaaa caatttgact gccaatgata ttaattttct tgttccccat
     9541 caggccaaca aaagaataat tgatgcaacc catgaaagaa tgggaattaa tgaagatcgc
     9601 gtacttatga atatagacag gtacggtaat acaaccgccg ggaccattcc attattgttg
     9661 tgggactttg aaaagaaatt caaaaaggga gacaatctta ttttatccgc ttttggtgga
     9721 ggatttacct ggggtgcagt ttatgtgaaa tgggcttata attcctgatt tttaaagaaa
     9781 actaatcatt tgtacttttg ctgaaaaaat aagaatggca actaccgcgg atttcaaaaa
     9841 tggtctttgt attgaattta ataataactt gtatacgatt gtggaatttc aacatgttaa
     9901 accgggaaaa ggtccggcat ttgtcagaac caaactgaaa aatcttaaca ccggtaaggt
     9961 aattgataac acatttactt cgggagtaaa aattactaca gcaagaattg aacgacgccc
    10021 tcatcaattt ttatacaaag atgatctcgg ctaccacttc atggacagca attccttcga
    10081 acagattgca cttcaacaag atttgattga tgctccacaa tttatgaaag acggccaggt
    10141 agttgaaatt atatatcacg ctgaaaccga gactcctctt agctgcgagt tgcccactca
    10201 tgtggaactg gttattactt ataccgagcc tggcattaag ggagataccg ccacaaatgc
    10261 ttcaaaaccc gcgacgctgg agacaggatc gcaaatccag gtccctttgt ttgttgaaca
    10321 agaccaaaaa attaaagtag atacccggac gggaacttat attgaacgag taaaatgagc
    10381 caaatgaaag ttaaagaaat tcaagaccta attgacttcc tttcaaaaac gggactcaat
    10441 gaagtaaata ttgagacgga tgaatttaag attcacatcg ttcgcgagcc aaaagttaag
    10501 caggttgtgg atactatgaa gaattcttcc tcagcttccg cttcttcatc ggttccggca
    10561 aaagccgaag caaactctga tgaacaacca aaaacttcca aagcatccaa attagtagaa
    10621 atacgttcac ctatgattgg gactttttac cgatcaccaa atcccgactc accttctttt
    10681 gttgaagtgg gagataaaat cgggccaggc cagactgttt gtataataga agctatgaaa
    10741 ctttttaatg agattgaagc tgaaatctcg ggaaccatag taaaagtact ggtagagaac
    10801 gcgtctccgg tggagtatga tcaaccgctc tttttagtag aacccaatta gttgcataaa
    10861 ccctgaatcc taatccgtgt ttaaaaaaat actaattgcc aaccggggcg agattgctct
    10921 ccgaattatc cgaacctgtc gtgagatggg tatcaaaacc gtggcaattt atagcatggc
    10981 agacaaggaa agcctccatg taaagtttgc ggacgaggct atttgtattg gtcctgcccc
    11041 aagtaaggag tcttatttaa gcatgcccag aattattgcc gctgcagaaa ttactaatgc
    11101 ggatgctatt cacccggggt atgggtttct ggctgaaaac tctgagttct cccaaatctg
    11161 ccaggattat aaaatcaagt ttatcggagc ttcagcaagg atgattgagc aaatgggcga
    11221 caaagccact gccaagtcca ctatgaagag tgccggagtt ccggtaattc ccggatcaga
    11281 aggattgctg agttctgtgg atgaagcgaa ggaaattgcc aaaggcatta aatatccggt
    11341 tatcctgaag gcaaccgccg gcggcggtgg tcgcgggatg cggatcgtga atagtgagga
    11401 acaaatgcag aaagcctggg atgatgcacg tcgggaatca ggtgcagcat ttggtaatga
    11461 tagcctttac ctggagaagt tcgttgaaga accccgtcac atcgaaattc agattgtggg
    11521 cgataagaat ggcaaggcat gtcatttatc agaaagggat tgttctattc aaagacgaca
    11581 ccaaaagctg gtggaagaaa ctccctcccc ggttgttaat caaactttgc gggagaaaat
    11641 gggacaagcc gcaataaaag gagctgaagc tattggttat gaaggagctg gaactgttga
    11701 gttcctggtg gataagcacg gagattttta tttcatggaa atgaacacaa gaatccaggt
    11761 ggagcacccg ataaccgaag aagtaaccga ttacgattta ataaaggaac agataaaggt
    11821 agcggctgga attacaatct ccggaaagaa ttattatccc aacctgtgtt caatggaatg
    11881 ccggataaat gcagaagacc ctgccaaaga tttcagacct aacccgggaa agattactaa
    11941 cttccacatg cctggcggtc acggagtaag ggttgacagc catgcctacg ccggatatgt
    12001 tgtgccgcct aattatgatt ccctgattgc aaagttaatc gtgagcgctc agacccgtga
    12061 ggaggttatc gtaagaatga aaagggcatt gagcgaattt gtaatcgaag gcataaagac
    12121 taccattcct tttcatctga aattgatgga tgataaaaat tttcaatcag gcaacttcac
    12181 caccaaattt ctcgaaagtt ttgatttttc tgatttgggt tgatttgact acgaggcttt
    12241 tgaaccaact taatcctttc taatctcctc aagattcgct aacttatttt gtcaatagta
    12301 gtgattttca attcatgaag gtttggataa taatctcttc atttattttt attggccaat
    12361 cccccgggta tcagggtaag gttgacagtt taacactaat tgcaagccag accactgtcg
    12421 ataccgttaa gattgacgct ttgtatgaaa tagccaaaat cttttatggc cagaactcaa
    12481 aagactttga tgtttttgcc ggggaattat tggtcttggc agaggaggta aattattccc
    12541 ggggaattgc cagaattctt tatctcaccg gattgtattt gcatcgagaa ggtaaattca
    12601 ttgaggcgtt acagctcatg aaaaggcttc aggaaattgg aagccaacca ggattaacct
    12661 tttataaagg acaggcttta gccttaaccg gaattattca taaggagaga gggcgattga
    12721 accaggggtt ggaatacctg gaagaagcgg caaagattta taaagctata gacgataaaa
    12781 acaggttgat taacgtgatg aatgctactg ccggcattta tactgatctg gccaaaatcc
    12841 atagcaatga ttcgaattat ctggaaattg gttatatgaa actaatggaa gtgaattcga
    12901 tggccaaaga attgggccac ctgacaatga ttgctaactc aaatgccttg ttaggagata
    12961 tttttcgaat gaagaaaaat tttgcaaagt caatttcatt atacaaagaa gcgtggttta
    13021 ttgccgattc tattggcaac aagaatatgt ccacagatat ttggggtgta attggttttt
    13081 ctttttacga gtcagggcaa atcgattcag ccagaattta cctgacccag gccattgccg
    13141 ctagtagaat gtataactat gaaccattat caataaaata ttctattgaa ctaggcagaa
    13201 cctatatctc aacaggtgat tatgcggtgg cccaaaatat tttaactcaa accgaaaagc
    13261 aagccaggag ccttgggttc tggaattatt tgaacgatat tattaaaatg aaagttgaaa
    13321 tttttagtca actcaaggat tttgaaaatg gtttatctgc catgtctgaa ttggatattg
    13381 ttacagatac cctgaataag atagccagtc gggataaaat agcattactg gagtccagct
    13441 atgaacttga taatgccgaa cttaaacttg cccaaatgga agaagaaaag gagtatcagg
    13501 cgaaaataaa aaaaattgtt ataggtggtt caattccact ggctgccctg gcaataatta
    13561 caattgtatt gctttggcgg tggaacctgg ggaaaaagaa aatgcttgcc actcaacaga
    13621 atttgcatca tcttgaaatt agcaatttgc aacttcagga aaagaatctt aaaatgaacc
    13681 ttgagctcaa gaacaaggaa ttggccgccc aggcattaaa tcttgttact aaggaaaatt
    13741 tgctaaaaga catcagggaa tcagtcactc agcttaatga tggtgttcaa agtcctgccg
    13801 caatacatgc aattctgaaa caaattgaat tgagccaaaa cagagataaa gattggaacg
    13861 aattcaaact ttattttgaa caagttcatg tcgatttttt taaggaactc aactcacgga
    13921 ttggggatct ttctccctca gaaataaaat tatccgcatt gctgagatta aacctgtcta
    13981 tcaaacagtg cgcggatatc ctcggaattt caccggaaag cgtaaaaatg gccaggcaca
    14041 ggttacgtaa aaaattgcag cttcaaactg aggataactt aatcactttc ctcatgactc
    14101 taaactagct agatcttcat ctctttccat ttgccttttt tgaaaataat aatcccggtt
    14161 attgccaatg ccgtttctgc aattggaaca gcggaaaata ctccattagc accgaatcca
    14221 aaggttgaag ataataaata tgccagtggg atttgaacca gccagaaaaa aatcaaattc
    14281 aaaatggttg gagtgcgtgt atcacccgcc ccattgaaag attgagttat taccattccg
    14341 tagccataga ataaataccc aagagcaata atctgtaagc aattggttcc gtaatcaatt
    14401 acttccttat cacctgtaaa aaaattcagt attggccttg aggaaaccaa aaaaactatc
    14461 attaccaggc caagaaaagc catattgtag tatcccgttc tccagacaga tttttcggcc
    14521 ctgtctggtt gtcctgctcc taagttctga ccgactagcg ttgcagctgc attggcaagc
    14581 ccccaggatg gcaagatggt aaaaattatc acccggatgg caatagtgta tccagccagc
    14641 gcggcactcc caaagttggc cattatcttt accagaaaaa tccagcttgc tgaacctatc
    14701 agaaattgag ctgtgcctcc tgcagatact ttcagaagcc ttttaataat atcaacccgt
    14761 acaaccaaat gcgaacgatt aactttcaga ataccctttc cactaaacat gtaatacact
    14821 tggtatgcaa cgccgattgt tctcccaatg ttagtggcta tcgctgcacc ctcaacacca
    14881 aaggatggaa ttggacctaa cccaaatatg aagataggat caagaataat attcaggata
    14941 ttggataacc aaagtgctcg cattgccata gcggcgttac ccgttcctct ataaattgca
    15001 ttaaagagga atagcagcat gacgctgaaa ttcccgatga gcatccattt tgcataattg
    15061 gcaccggtac ttaaaatccc ttctgtagct cccatcatgg ttagcaaatc acgatggaaa
    15121 aagaacccgg ctacactgaa gattagcgcg gcagcaaatc caatatataa agattgaatt
    15181 gccgctatag ccgcaccctc tttattcttc tcccctatac ggcgggctac cattgctgtg
    15241 gcacccattg aaaggccgat ggctatagaa tagataatag taagaataga ttcagtcaaa
    15301 ccaatcgctg aaacagcgtc agtattattt aacctgctga catagaagat atccaccact
    15361 gcaaagagtg attccatcgc catttccaaa accattggaa gtgacaacag aaaaatagcc
    15421 cggttaatac tgccttcggt gaaattttct tcttcccccc ggattgcctg cctaaccagc
    15481 ctgaaaaaag aaataagttt actattggag ttcattaaac gtacattaaa agttggaaaa
    15541 agcctgtgcg gcgaaattga tcggaggact tacctctatt ccaggccttt catacctggg
    15601 tctatctgac cagcttcata attctgtacg ttttaaattt aagcagcgta aatatggctt
    15661 aattcacggt aattcacaag tagtaaaaac tctattaaca aattatttgg atttcgatga
    15721 gaccttttcc ggttcacttt gctgaataag cgcaattaat tcctcatacc attttttgcc
    15781 gtaacgtcta atcaaagcgt ctttcaaaaa aacataaaga ggaactttga gagcttcacc
    15841 attgctgcag gcgggaccgc atatatgcca tcggttataa tttatcgctt cgtattcctt
    15901 gtatttagtt atccgaatgg gatataagtg acaactgatt ggttttctga attttgattt
    15961 ccctgccaaa aaagcctgtt caatcccaca tttcaaaatt ccccgttcat cataaacaga
    16021 ataagcacat tctttaccct taattgttgg ggtagagtaa tcgccgtcag agtccaatac
    16081 atattcaccc tgcttttcaa tttctttaat tcctgcttta gcaagaaacg gttttatctt
    16141 tgggtattct cttttgagca agtcaagttc atcatcctcc aatggcgctc ctaaatcacc
    16201 ttcgacgcag catgcaccct tgcacttaat caaatcacat acaaagtaat tctcaataat
    16261 gtcttcagaa atcagtgtac ccttatgtga aatcatggtg ctaaaatagc tgaatttgat
    16321 tggatgacct gcgtcggact tcagttacta ctttagaaac attggctttt ctgcacaaaa
    16381 caccctggta gtaaagagaa ttcatttttg aggattccgt taatgagaat gagcccttaa
    16441 ttcgaaagtt attttcataa gtccattttt caatgtttct gatatgattc ctggcataca
    16501 attcaaaatc atccttacct gatttcaact tggtccaaaa ttttttcttt tctccggcaa
    16561 gtgtaaaaaa tactacagga tctaatgatt cgatagaatc aaaaaaatga atttcaggaa
    16621 atccaaatct cgggttcaca ttacaaaaaa tccagggttt aattcctttc gtatttatgg
    16681 ggacataatt tctaacttgc tcaagaaatt tttcccacga agtactgatt atcagcacat
    16741 ttttatcttt gaaatttctt ttgattctaa ctcgcaaagc ctgggcatac gtttcattag
    16801 gctcccaaaa gatgtaacga ttaaactgaa ccggcgatga gaacgccagc aaacttgtat
    16861 cataaataca ttcgccggta ttattcattt ccaacattcc tgttcctgca ctcaagtcga
    16921 gataaatccg gtgattataa gcagttgaaa gcaagaggga atacacttca agatagtttc
    16981 cgagggtttt gattggagcc aggatccagg agtcagattc cagagccaga aggccatcct
    17041 ttttcggagc aatcaaagga taaaattcgg aattggacat tcaataatgt ggttttgcaa
    17101 aagtaataaa gaatgagggg tttctgtcca atttcgctta ataattagaa tatcctctta
    17161 caaaaaaacc aattgtgcta tcttgcggaa atagtttttt ctgattgaat ggagtacctg
    17221 catagcctga atgaagcaca aaaagagggg gtgataaaca ccgaggggcc atgcatggtt
    17281 atcgcaggag caggctccgg aaaaaccaga gtactcacct atcggatagc tcaccttatc
    17341 aaatccaaag gagtggatcc attcaatatt ctctcgctca cttttactaa taaggcagca
    17401 ggggaaatga gaaacagaat tgaatccgtg gcaggattgg atgcacgaaa tctttggatg
    17461 ggaacttttc attccgtttt cgcacggatt ctccggtcgg aaggcacacg gctgggttat
    17521 cccaacaatt tcacaattta tgatacggat gattctaaat ctttgataaa atcaatcgta
    17581 aaggaaatgg ggttggatga aaaactctat aagcccggta ttgtttacaa tcgaatttcc
    17641 ggcgccaaaa acaggttgat tggctgggaa gattatcaaa acaatccact ttatttggcc
    17701 gacgatgaaa gcacccttcg ccccgaaatt gccaacatct accggaacta ttgtctgcga
    17761 tgcttcaaag cgggcgccat ggattttgat gacctgctgc taaagacaaa tgaactattt
    17821 gaaaaattca ttgaagtgct gaacaaatat caacatcgtt ttcagtacgt cctggtagat
    17881 gagtttcagg atacaaatct ttcccaatat aaaataacca ggaagctcag ctcagttaac
    17941 cagaatattt gtgtagtggg tgatgatgcc cagagtattt acgcgttcag aggagccgat
    18001 atacaaaaca ttctgaattt tgaaaaagac tttcctgatc tgagaatcat caaacttgaa
    18061 caaaactacc gctcaacgca aaatattgtt aacgcggcaa attctgtcat aaataacaat
    18121 cgtgctcagt taaaaaagaa tgtatggacc actaaccccg aagggtctct catagaatta
    18181 tttaaggcaa actccgataa tgaagaagga aggcttgtcg catcgtcagt ttttgaagaa
    18241 aaaatgcaaa atcaattgca taacagtgac tttgcaatcc tttaccggac taacagccag
    18301 tcgcgcgcaa tggaggaagc cctgcggaga atgaatatta agtataaaat catcggcgga
    18361 ctttcattct atcaacggag agaaataaaa gatctacttg catacctgcg attctccgta
    18421 aatcacaacg atgaggaatc acttaagaga atcataaacc tgccaaaacg gggtataggt
    18481 gacggaacga tcagcaaaat tatggttgcg gctgaagaac atgcaattcc ggtttgggat
    18541 gttctttgca acataaaaag ttttatgagt ggaagagccg ctaatgccat agaggatttt
    18601 actatcctga taaaaagttt tgaaattgaa atcggaaaga aggatgctta cgaagttgca
    18661 agtcatattg caaaggcttc aggaattctg aaagaacttt atcaggataa aacaattgaa
    18721 ggacttaacc ggtttgaaaa tgttcaagag ttactgaatg cagtaaaaga gtttgttgat
    18781 aatcaggaaa atgaggagaa gaatttaaac acttttttgc aggaagtggc attgcttacc
    18841 ggcattgaca atgataagga tgaagataat gacaaggtga cgttaatgac cattcacatg
    18901 gctaaaggac tggagttcaa aaatgtctat attgtgggat tggaagaaga tctttttccg
    18961 tcacaaatga tgttgacatc acgggctgat cttgaagagg aaagaagatt gttttatgtt
    19021 gccattacaa gagcaaagga gaaactcttc cttagctacg ctgtaacacg ttatcgattt
    19081 ggaagactta agagttgcga gccaagcaga tttcttgacg aagttgatcc gcaatatatt
    19141 aaagagaaca ggaaattcaa cacggttcaa aattactcca catccccggc caccaactat
    19201 gcaaggaatt ttgttaataa tatccgtaaa acatcctcac caaaacaaac tccggcaagt
    19261 taccagacac ctatcgattt tgaagccagt gacaccacaa gactaaaagc aggaatgaaa
    19321 gttgagcatc ccaagtttgg ttttggtaag gtcatctcca tggaagaaaa cggaattaat
    19381 cgcaaggcta aggtgcattt tgacgggatt ggacaaaaaa ctcttctttt aagctttgcc
    19441 aagctaaaaa ttcatgagga gtaaacttct ggtatctatt gaattatttg gtaagtttgc
    19501 accagactta aattgatcga tggaaaagat ttttgaatac aacaccgaaa ggaagaacct
    19561 gatactcaaa gagtatggaa gaaacatcca gaaactggtg cattacctta agaccattga
    19621 ggacaaggaa aaaagaagcc agatggcagc cactctgata gaactaatga agcaaataag
    19681 tccttcaatc agagaaaaca atgaaaccaa tcaaaagctg tgggatgatc tttatattat
    19741 gtcaggattt gatctggaaa tagacagtcc ttaccccatg cctccccttg atattctgaa
    19801 taagaaacct ttgcgacttg gatattccac taataatatc cggttcaagc attacggaaa
    19861 gaatctggaa ctattgattg ccaaagcaat ggaacttgag gatccggagg aaagggaagg
    19921 cgcggttgta tatattggga aactcatgaa aagtttctac agcacctgga ataaagacaa
    19981 tattgaagag gaatcaatcc tcaaaaatat acgtgaactt agcaagaatg agcttgacat
    20041 tgatatcacg aaggttaagg aaaacaattt atttgaatca ctatacaaag agcgccccaa
    20101 gcctcctcct cagcacacca acaggggcag aaagaatcaa agaggcggtg gcagtaaacg
    20161 cagaagataa ccatgagttc tttccggata gttgggggaa atcaacttaa gggagagata
    20221 attcctcaag gagcaaagaa cgaagctctt cagataatct ccgccactct tttaacgccg
    20281 gaaaaagtta ctattcacaa cattcctgaa atcagagatg ttcaacgact gattcaatta
    20341 ctcaaatccc tgggagctca ggttgaagat aaaggaaagg gtacttttac tttcgcggct
    20401 tcaaacatcg acctgggttt tctggatagc cccgagtatc tggaaatggg atctacacta
    20461 cggggctcca ttatgattat ggggccgcta cttgccagat ttggcaaagg aaaaattccc
    20521 aggcccggtg gcgataagat tggcagacgc cggttggata cacactttat tggatttcaa
    20581 aaattagggg caaaatttga atatgatccc ggtactggtt tctataccgt ggaagcaaat
    20641 catctaaagg gcacgtatat gttgcttgat gaagcttccg tcaccgggac cgccaatttg
    20701 atcatgactt ctgttttagc ggagggtaaa actaccattt ttaatgcagc ttgcgagcca
    20761 tatatccagc aactttgcaa aatgttggta cgcatgggag caaacattga tggtattggt
    20821 tctaacttga ttatgatcga aggggtaaat aaattgaagg gaacagaaca cacacttctt
    20881 cccgatatga ttgagatagg aagttttatt ggtcttgctg cgctgactca atctgagatt
    20941 acaatcaaaa atgccggcgt taatcacctt gggttgattc ctgaagtgtt tcgaaggatg
    21001 ggaatccaaa tgttaattga cggtgatgat atctttattc ctgcccagag cgagtatgta
    21061 atagatactt tcatcgatgg ctcaatctta actatttcgg atgcaccctg gccaggcttc
    21121 actcctgatt tgctcagcat cgcgctggtg gtcgcgactc aggccagggg tacagtttta
    21181 atccatcaaa aaatgttcga aagcagatta ttttttgtcg ataaacttat tgatatgggg
    21241 gcgcaaataa ttttatgcga tccacaccgg gctacggtga ttggcctgga tagaaaatat
    21301 ccgctacgtg gaattaatat gtcttcccct gatatcagag ctggggtctc tttgttgatt
    21361 gccgcactct cagcggaagg gaccagcatc atttataatg gagagcagat agaccgcggt
    21421 taccaaaata ttgacggcag gctgcgtggc cttggtgcag atattcagaa agttgattaa
    21481 ttaaatttcc ctatacgtaa ttattgagca taacaggcat cacaagcatt aagatgtctt
    21541 cattatcctc cttctccctg ggtacaatca ggcccgcttt attaggtgcc gagaattgga
    21601 attcaacttc tttagcatcg ccattagaaa gcatttcgat caggaatcgg gcgttgaatc
    21661 caatctcaat atcctcccca tcatgttcac aggaaagtct ttcatttgct tcattggaaa
    21721 aatccagatc ttcagcacta atctggagtt cactgccggt aatctttaat cttacctggt
    21781 gggtggtctt attcgcgtaa atggcaattc gcctcattga actcagaagt tccgccctgt
    21841 caattaccat tttatttttg ttatcctttg gaattacatt ttcatagtcc gggaatcttt
    21901 catcaatcaa ccggcaaatc attttaatgt tgccgaacct gaaaaaggca tttgccatat
    21961 tgaattcaat gtttacagga acctcatcaa ccggaagagt tgtcttcaac agatttaaag
    22021 cttttcgggg aattatgatg gcacttccat tctctgattt cacatctgcc ctccggtagc
    22081 gaatcaatct gtgaccatcc gttgctacaa aggtagtgtt tgagtcgctg agattaacgt
    22141 aaactccggt cattgccgga cgaagttcat cattactggt ggcaaatatg gtactggcaa
    22201 ccgctttggc cagaacacca gaagacattt ccacagaaaa gtcatccgaa acagagggaa
    22261 cttttggaaa gtcagtggca ttctccccgg aaagcttata gcgaccgtta tcagaactaa
    22321 tctccacact gtaagtttgt tcgtcgatac tgaagtttac gggttgttcc ggaaggtttt
    22381 taagggtatc cataagaatt ttagcgggaa cagcgatgct tccactttct tttgattcaa
    22441 tttccaactc tgtaatcatt gaagtttgaa ggtctgaagc tgttatggtg agcttgctgt
    22501 cgttaatttc aaatagaaag ttctccagaa ttggtacaac aggattagta gtaataactc
    22561 catttatgcc ggaaagctgt tttaacaggg ctgatgagga aactataaac ttcatagatt
    22621 ggaaattagt gatgttttaa aacatcggta aagttaaaaa taaaatcaaa attacatagt
    22681 gaaaactcag ccgcgagcca aaattgctat tcccagtgtt cattatgtat caaaaccggt
    22741 cccagggatc gaggattgaa cagaaccaac aattccccat tataccgcgc taaagttaat
    22801 cgtctttcct ttattgttgg aaatacctca agaacctgat cattggaacc gatagtcaca
    22861 aggacaactt ttgccagggg aagtgatgcc gccagactat cataagccag gtcttcttca
    22921 ggcaaaagaa atctctcagc ctgaaggttc atgagctcac ctatataatc aaatattaca
    22981 gcactatcaa tgggttgcga attattattg agataaaaaa aatcttcttt ataatttaca
    23041 ctgaatcctg gtgattcgtt attaatatat tcaacaagaa ttgatttgag gcttgaccag
    23101 tccagctcaa aaaggaatcg gtttcgccaa tctttaagtg ggacggaata aattcctgct
    23161 acatagcttt tataaccggg cagtataact tcataaggcg cctcatcatc gctgtccata
    23221 aaataggaga cgccactcag ttcatcacct ccaaccaaat aggatttaat caaattctga
    23281 ttattaatga tccgcacttt ggagccggag gtttttagaa aatttataag ggagtctttt
    23341 tgattgatgc cggccggttt ttttactttg atggagctga taacagtaaa gaaagcatcc
    23401 cgcattcccg gatccatccg gaaggaatca tttacgatcc actccccttc caagaattca
    23461 aaattcaaat ttaccccggg agaagaaata tctactgaag taataatgga gttcacttcc
    23521 aatgtaaatt tattttcatc aaaattctcc ccgctcctct cacccgatgt atagacaaaa
    23581 aatgttaatc ctgataaaat taccaggctt gcaatccaat aaccattgtg tattctccta
    23641 cttaaaattt gcatatctcc tcttcctcag gtaaaatctt ataattccgt aagcaaataa
    23701 aagaacaagg ggtgtaacca gattcaaagt ttgccaaaat cctttttgct cgccaatttt
    23761 cactttatca agcggacgaa tttgaacttc tttggctcgc gctgtgatca atcccttgcc
    23821 atctaccagg taatccaccg catttagcaa gaaatccata ttaccaaaca tctgcctgga
    23881 atagggatca taccctaatt ccatgggtct gccggttgac gaattgattt cattcctcgc
    23941 gatatctcca tcggtgatta ctattattcc tgtattacca ctttctcctg ttatttcctg
    24001 atctctatca atgcctggcg ggggaaatct gtttttgtaa agagaagtaa aagtaccctc
    24061 tagcaaataa gcgactggaa tatttttctc attcatttgg tccggagtga gttgaatttt
    24121 caagtcttcc gcgctaaccc ggaccggagc tgataatctt ctcgtatatg cactggtata
    24181 caataacggg atttttgcta cacctgtagc ttttaccgta tcaatactgc tgacaaatct
    24241 tccgtaaatc gcgtcaagat ttcttgtaat taaatgagcg ctgaagttat ttaatatcgg
    24301 gttgaacggc cagggcatta attgaatctg gggttgatct cccatggagc cgataacaat
    24361 tggctgagat cctgaaatta catcctggat gagatcctga ttcaggcgaa ctccatattt
    24421 gaataataag tcctgaagtg ccagatcata cccaaaagca aatgttcccg tttgaatgct
    24481 atccatctta acattgagca tatccagcag aaacattgcc cggccaccac gcataatata
    24541 ttgatcaatt ttgaacttat cctgatcaga aaatgccctg accggtttaa taattattat
    24601 taagtcggta ttcgggattt caatggtttc cggtaagtct atggattgca cccggtatct
    24661 ttctgacaat gttgacctga aactaataat attaatgcta tccagttcac catgaccggt
    24721 aatgaaagaa atggccggca ccatatcatt agttatggtt ttgatagttg atgcgatttc
    24781 atactcgata ccctcaatgc tctgattcaa tcgtacttcc ggagcagccg actgattgcc
    24841 tttgaacaac tgaactcctt tctcggccag gccataagaa acgaccgcac cgggcaagat
    24901 ccgtttttga atccggtttc catccttatt catgaaaaca tcataaggca atattccttt
    24961 ttcagaaata ctttgaaaaa agttttgcct tgattgcgca tttcctgctt gttcaggatc
    25021 aataaacctg aagcttactt tataatccga atatattctg aactcttcca atatctcccg
    25081 tattgatttt tgcattcttt caaatgccgg gggcaattct ccgccgaggt acacttctac
    25141 cagaatatta tcgtcaagag aagttagaat ttctttggtt gcggttgaaa tggagtatcg
    25201 tttttcctcg gtcaaatcaa ggcgaaaaaa atttcggttt gccatgatat tcagaagcaa
    25261 taacatgact gccaaattaa gaaacttaag aatatcgcca acctttctgc tattcatgat
    25321 ccccattgtt tactctttaa gaccaggtta gttgatccaa tcattattat gattatggtg
    25381 agaaaataaa ccgcgtccct tgaatcaata agtcccttgc tcattgaaga gtaatgataa
    25441 ctgattccaa gttgttcaat aagtaaagaa ttaccttcca cgccaggcat tattgatatc
    25501 gatgaaaagc cctggtacat cacgaaacaa aaaaatactc ccaggatgaa agcgacaatt
    25561 tggttcctgg taattgagga agcgaaaatt ccaaatgaca cgaacactcc accaaggaga
    25621 atgagtccca ggtatgagcc cataacctct gctgaatcaa tatttccggc aggatttccg
    25681 agggaatgca tggagtagta atacactccg gttggaagca aagcaacgat tacaagagtc
    25741 aatccggata agaatttccc aataattata ctcattccgg aaaccggctt tgtaagaatt
    25801 aattcaagag ttccggattt tttttcctcc gcgaacatcc gcatcgtaat agcgggcaca
    25861 agaaaaataa aaatataagg tgaaaatgaa aataaagttt caagatcagc atagccataa
    25921 tccagaacgc tggtttcggg aaagatccag acaagcaatc ccaacccggt aagaaaaaca
    25981 gatattacaa tatatccaat aagtgaattg agaaagctat ttatttcctt actataaata
    26041 tgaagcataa ttattcctgc cgcgttagtt cctgaaaaat agattcaatg ctgctttcaa
    26101 ataattttag ccccactaca tcgcttccca attccattac tttcctggaa atcaatggcc
    26161 ggagatcttt ttcaaaagat gcagtgattt cataatgtcc attacctttg ctgattacgt
    26221 tattcactcc ttctatttcg gcgagcctgg ccatatcaat ttcaggaata acttccaact
    26281 caatccttgt ttctttcccc ttcattcggg taagaacgtc aatggtatca ttagcaacaa
    26341 tctttccttt attgatgatc actacattac tgcacaacgc ttgaacttcc tgcatgatat
    26401 gtgttgaaaa cagcacagtt tttctggagc taacctcttt tatgagttta cgtatttcca
    26461 gaatctggtt gggatccaaa cctgtagtag gttcatccag aatcagaacc tcgggatcgt
    26521 gaaataatgc ctgggccagt ccgacccgct gacggtaacc tcttgaaagt gaatccagga
    26581 gcttattttc ttcctctgct aaaccgcaaa gaataataat ttcttccact ctttttatta
    26641 attcattccc tctgaagccg tggattttcc cgacaaattg caaaaattca tgtacataca
    26701 tatccaggta aagcgggttg tgctcgggaa ggtatccaat aattttcctg acttccgctc
    26761 cactttcctg cacgtccagg ttacatactg aaatattccc ggaggttggc ggaagatagc
    26821 cggtaatcat ttttaaggtg gtagattttc cggcaccgtt tggcccaaga aaacccataa
    26881 tttctccctg atttacttca aaggaaatgc catcaactgc tttttgctta ccgtaaattt
    26941 ttgttaaccc ttcaattctt attgacatga atctgcattt gcaaggcttt caaatatacg
    27001 ttgtttttca cgttttattg ttttcctttt ggtctgagcg gcctcacttc tatgtcaacc
    27061 gggtgatagt ttcctgaagt ttccaaacat tggattattg tgccggctat atcttccggc
    27121 atcatcatat tatcagaaat ttttaccgag cttatttcat caaaaaagtt tgttttggtt
    27181 gaacctggat agatgcaggt aacttttatt ccgtaatttc ttaattcctt gaacatcgat
    27241 tgcgacaagc cgcgaacagc atgtttggtt gcacaatagg cagacatatt cacaattcca
    27301 ttggtgccgg caattgaact gatattaata atatgacctt cctccttctt tttcattcca
    27361 gggataacca gtctggaaca ataaaatatt ccatccacat tggttgaaaa cattttttgc
    27421 cacagatcaa ggggagtttc ttcaaaagga tattcgtagc caagcccggc attgttaatt
    27481 agtatatcta cctctacccc aaaacctttt atggttgctg cgtaaccatt ttcaactgac
    27541 ttaagattgg agacatcaac agcaaagaat tgaaagtttt tgtgctccaa cccgggagcc
    27601 gtgcgagacc accctgccac tctcacacct ttgcttatta aaagcttgca ggtttccaac
    27661 ccgattccct tcgatgcacc ggtaacaatg gctattttat tttcaatttt catttcatct
    27721 gaatcgatat accaaactca accccttgaa ttcgtttcca taaaatgggc ttaaggataa
    27781 gttaagattc ttttcaacat tccggtcatg aattctggaa atgtaccggc cggtaaagta
    27841 tcctaatgca aatccaaata ccacatcagt tggccaatga cgtaagtcat ttatccttga
    27901 caaagaagta agggttgcaa gactatatgc aaccggggca acccacatct tatctttgta
    27961 ctcataggca aatacggcag ccacagacca gaccgctgaa gtatggccgg aaggaaatgc
    28021 ccgatgaccc cggaatgggc ggtcgcggtt tactgcaaat tttatggcct gggtgaaaaa
    28081 accggagata gcaaaacttt gcaaggcaac caatgatgat ttttttaacc ggtaatcttt
    28141 ggttaaaacg ccaatgccat aagtaccgaa gagcacagaa aagggcatta atacgctact
    28201 atttccaaaa ggttcgaaaa tatccgatac cgtgttggat tcgtttgatc gtatttcgct
    28261 cacccaatcc acaatatctt catcaaaaat atataaaaca aaagttgccg cgcccgtcat
    28321 tccaaaattc ctccaatctt tttcttccca gttcgccggt gacttaatca ttgcccaggt
    28381 attggtaaaa tacccacgta tatacttgcc gttgagtttc acactgtcgg gcaactcttt
    28441 attctgacta caaaccagac cggaagtagt cagcaaaaaa aaggaaatca gaaaaattat
    28501 atatataacc ctattcacta ttcttctttt ttctgaaaag attttctgta tgataaatta
    28561 ttgaaagttt tagagaagtg taatcaaagt cgttgaatcc aagttggtac tgggcaagat
    28621 aattaaatgt ctttgctttt cgcgtgtatt ccaaacgata aacaagtggt ttatcgccat
    28681 tatctttcca tcccgcgtaa ccggaaagag aagttgagaa tgaattttta tttagaatca
    28741 gatccaatcc caggccatac atgggtgcgt cattctgagg atgctttctg tcataagttt
    28801 gccaggcgta gaaaccgagg ttggcgtaag gtcgcagcca tttgttttct tcacccaatt
    28861 gaaaatcttt cccggcgctt acatcaatga agtatccggg ggaatcagta aacctggcac
    28921 caccggtttc cgatccgctt gcagttttgg ttacggcccg cagcaacaaa tccgggcctc
    28981 ttggccggtc tttcaaaatt tgaatttcca tgccaaaata tacatcgccc ttcgcgaaac
    29041 cttccggttc aaaatattct cctgaaagca ttctttcgtc ccttgtttga tcggagacat
    29101 cgaaatgttc ctggggaatt gcataaatga ccagtgatgc aatgtcttta gcaagattaa
    29161 cattcagata cgtccagagg ttttgggtat tatcacccgc tccaaaatga gcctgcgctc
    29221 ccaattccag gctaagttca ttatcaactc ttccttttgc tacggaaggc acaggcaaag
    29281 cgtttggtcc cattccgccc ggtgaaatcg aaatatagtc aacccagtta tcggtaggac
    29341 atccaatggc ttgaccggtg ttagtgcacc accaatccca gttcacctga ccttgtgcaa
    29401 aactctgacc tgaccctacg attataggaa gcacaataaa aattcctgcc acaattttta
    29461 tataaattcc caccgtcttt ttccctttca tattacgcct ggattccctg gattaattct
    29521 ttcataattt tagtcattcc cggttccgcg gttttagcgg cggcaatcac ttcttctacc
    29581 gtaacttttt ttatttttcc ttcgattccc aaatcagtaa tcactgaaat ggcaaaacaa
    29641 tcaataccca tttggcgggc gactatattt tccggaattg tagacatgcc gaccgcatcc
    29701 gcccctagtc ttctgatgta ttcatactca gcaggagttt ccagcgcggg cccggccact
    29761 ccggcgtata cgcccacatg agcccggata gccaggcgtt tggcaatgtc caccgctttt
    29821 gcaatcaggt cccggttata aggttcgctc atatcaggaa accgcggccc aaactcctcg
    29881 ttatgttccc cccgcagggg actatccgga aacaggttaa tgtgatcttt cattatcaat
    29941 aaatcactta tttcctgcgc gctattaaga cccccggacg cattagaaat aaataaatgc
    30001 gttataccca acagtttcat aattcgtacc gggaaagtaa cctgttccat attgtaccct
    30061 tcgtaataat ggaaccggcc attcatggct actacctgct tatttcccaa cgttccaaaa
    30121 agtaatctac cttcatgaaa ttctactgtg gaaacctgga agtgcgggat atcgctataa
    30181 gaaatttcag attttatatc aatttctttc gccaggtccc caaggcctgt gcccaggatg
    30241 atcccgattt caggagagtc gctaatcctt ttttttatga aatcgagggc ttcaaaatac
    30301 ttcgatggaa tcattccgga attcaatggg gctggttatt aaattttgac tcgaaaataa
    30361 tggaggcagt gaacaattag accaaattac gccaaaaaaa cctgttttgg attgaagaga
    30421 tttctttaaa aggatagaaa gggaagtaat tagcgaagtc cccctccctt gagatgactt
    30481 ttcatcatcc actggagtaa aataaaagtg agcaccaggc ctgaaacgat tccctcgaaa
    30541 gcaatcacga atgcaagcat gtacgggttc aggaatctgc caaactcatc ccggttccgg
    30601 atgtattcca tcagcgcggg atcaagttgg aggtagataa aaataaataa tgcaaacggc
    30661 aggacggcaa ccgaggaggt taagcagcca attccccaac cctgcaggta tcccattggt
    30721 tgctcattat ttttttggaa ggcttttaag gctttgtaaa tacctattac cagtaccagg
    30781 aaattcaagg tccgtagttc tacgatatgt accaggccca cgaatttcat gattaaaaaa
    30841 tatgccagca aacctacagt ggtaagtaat ccaaaccgga cagaaatact ctcaattgaa
    30901 tatttggtgt cttgcataat gaaaaatgag gaaggtttct aaatgtaaga atctttgtac
    30961 tcaaataaca gatttttatg agaaattaaa tccaaagaaa atcctgccag tatgataatc
    31021 aaacccgctc taattcagtg ccttttaggt gtgccacact ttgctgacat tgaaaatcaa
    31081 taaattttca cctaataaaa agtggaagtg tggcacacct tgatgcacat attcgagggc
    31141 atgtaacaga gggcctttta ggtgtgccac actttgccga gattgaaaat caatcaattt
    31201 tcacctaaaa taaaaagtgg aagtgttgca caccttgatg tacgtacccg agggcatgta
    31261 acagcaataa caatggctat gaaaatgtaa atgtcgattt tagagtggtt ctaaaaaggg
    31321 caagttgctg ctaagggttt gcgggtaagc aaagttgcct ttttaagcgt taggttcgat
    31381 cggtctattt tgctacacgc tgtcaggctt tggcggttac aatctgcttg tcaagaatgt
    31441 ctctggtgtt agcacctgta tatgtgcttt tttatagtct ttcgtatttc tggttattaa
    31501 aattgactga ccattttcaa ttgcagtatt atattgtata gcatcttcaa agtctgcaaa
    31561 gtccgatgca attgataagt ctatggtctt gctgtcaaaa ggtaaaacgg ttacaagagt
    31621 tttaaactta gcaagcattt gccttgaact tttaccagaa aattgcgaac gcaaaacata
    31681 gtcaatattg gcaaatgaaa ttgatgaaac aaatattttt attttatgat tgtcggcaag
    31741 tgaaaatagt ttagcggcat gaatattaaa tggcatacgt tcagatagca agtcaatgca
    31801 tacgtctgtg tctacgaaaa ttttaatcat aattatttag aatatttttt tagaatatgg
    31861 tctttatatg atttcatgta atcataattt ttgggaaggt ttaatacacc ggataaactt
    31921 ttgaccaaag gagtaatttc accagcttcc atattgcctg atgttaagta atctaaatat
    31981 gtttcaatta gcttagaaag actcgtcttt cttttcttcg cgtaaacttt tgccttttca
    32041 atgatattat cgtcaagctt gagtgtcagt tttgtgtcca ttgtcaatag aattaattat
    32101 acgtgcaaat ataactaatt tatacgtatt tatcaagaat tgcaggattt taaccgctta
    32161 agtattgttc ccctggaaat atcttcggct gaatatacaa tgcctgccat cactggatta
    32221 tgatggatat tatcactaat gattccagga agtttttaat ggtaacatta ttagaatctc
    32281 tcaaaatatc agactgccta aacccacttc agttgattgc aataaatgaa tgtgacgact
    32341 catgttcatc cggtattctc tatataaatg gagttaggaa aattatccaa taatttcgca
    32401 tgctgctaac ttgtcctgct acaattgaac cttgttccag gcgagtgatg gataagaaca
    32461 tcactcaaaa atagttagct aaatttatcg ctttaaccca gccaggagaa cgccaaaatt
    32521 tttgggatat aattaccaga ttttggaacc agcttaccat tcaccttgat ttcgaatagc
    32581 ttcatttgtg acaaattatt attcaaaact aaaactttaa tcaggcatga aaacaatcaa
    32641 ccaatcaaag gggctactta tatttatagt actcctcaca gtaatttttc aaaaggcatc
    32701 ttgccagatt tcttcattcc cctaccttga agatttcgaa gctgatgatg gaggctggat
    32761 cgcgcagaat aatggagccc ctaatggttg ggaatatggg actccaaccg ggttctcttt
    32821 aaattctgcg cactcaggca taaaggtatg gggaacgaat ctggatggtg attatgataa
    32881 caacgccaac ctttatcttg agtctcctga gtttgatttt tcaggaatgg tgaccgatcc
    32941 ttatttctta atctccacag cctttattat tgagaattac tatgatggag cttatatcga
    33001 atattcaacg gatatgggcg taatctggac agtccttgga gatttgacag ccggtcaaaa
    33061 ttggtataat aaaacattat ccaatggcga aggatggtca ggtagttacc cggattggag
    33121 tgttgccagt atcagactct ccgggctggc ggatcaacca tcggtaatgt taagaatcgt
    33181 ttttacctct gatgaatcta taacgagaga aggtatattg attgatgatg tccaaatagt
    33241 ttccaatttc acggacatga aaatatcggc tgaagaaaat aatattctcc tcacatctga
    33301 gcagagcacc attaatgtag ataacattgg aactgacatt tctactccat tctcaataca
    33361 agtcgaaata actagaaatc tggaagtctt acatcaggaa acttttcaaa atataaccct
    33421 gacggaggga caatcgctag cgctaaaccc tgcttactac tttgacgagc ctggcggata
    33481 taccttaaca gcaaccgctc aaatttctga ttttattgaa tccaataatg gttttactaa
    33541 gaaactaatt gttattggtt catcaggttc tacattacca tatactgcgg gatttgaatc
    33601 cggagatgcg gattgggtta cggaggatca ggttgtgaac tcatgggaat tgggatatcc
    33661 cgcgggtaat gtgctaaacg ccccacatac gggccttaat agttttgtta ccaatttaga
    33721 tgggaattat gataataatg ctgtattaga attatatagt ccattatttg atttctcatc
    33781 agccaatggg tctgaaagtt ttcaatttgc aattaaccat gaattggaac cgggatacga
    33841 ttttgcctgg gttgaattct caaccgattt aggtgaatcg tggtcaaagc tgggaacaaa
    33901 tttgtcaggt ggcaacaatt ggtacaattt tgtccaaggc tggtcggaaa attctgcggg
    33961 ttggaaaacc gcgtcccact ctttgagttt tttttcaggg gaagctactg tcatactcaa
    34021 aattgtagta agctctgatg gcatcgccgt taaag
//
LOCUS       DASWHX010000203        28915 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_2814930, whole genome shotgun sequence.
ACCESSION   DASWHX010000203 DASWHX010000000
VERSION     DASWHX010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 28915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28915
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2814930"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2035
                     /locus_tag="VGA21_13355"
     CDS             <1..2035
                     /locus_tag="VGA21_13355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase M14"
                     /protein_id="HEX9651544.1"
                     /translation="QEYVGHDNNRDWFMSNMPETKAATTVLYNQWYPQIVHNHHQSSP
                     AWARIFIPPFRSPVNDKIHPGVTTGVNMVGAAMASRFVSKKMPGAISQTTYEMWWNGG
                     MRTVPYYHNMIGILTETAHTTPTPRYYPPDSIPKMVGGKTPNGTEIFYPYPWKGGESH
                     FRDPINYMIEASMAILDLASSRKEYFLKGIYDMGRDAIEDEAGAYAYVIPKNQWDAGE
                     ANNLVNILMQGGIEASQATSKFTAGGQEYEAGSYVLYGAQAFRPYLADLLEKQNYPEL
                     YQYPGGPPISPYDLTGWTLPMQMGVKVDRIDQSFSANTRVLTQQIDVTAGKIDGSATY
                     GYALSNNENRSYKAVNRLINAGEKVLISDQPFSDGKSNFIAGTFIIEKGNNTENLVKT
                     LTRELGVTIAGIKTKPSGNLRNLGKVKVGVYKSWQANMDEGWTRFVLEQYDFDLDTLH
                     NEDILSKNLSAYSAIIFPAQSPNGIMNGHEPGSMPEKYVGGIGIEGLLAIENFTKQGG
                     VLIMFDDASDLAIEQFGLPVDDITSNLSSSQFFIPGSIVRIKNDLNNPLAYGMQEETG
                     ASFQRSKAFNVVSQRRYAEGGREETKPAEKPDVEIVTRYASSEILMSGWGNGVNRLKN
                     MGAVMNVGYGNGRIILFGFRPQFRAQPRGTYKLIFNSIYLGASKQANGNNKSE"
     gene            complement(2086..2343)
                     /locus_tag="VGA21_13360"
     CDS             complement(2086..2343)
                     /locus_tag="VGA21_13360"
                     /inference="COORDINATES: protein motif:HMM:NF015069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET family transporter"
                     /protein_id="HEX9651545.1"
                     /translation="MHWTELVGHFGALLSSITFIPQVYKAWKTKSVKDLSMTMILIVF
                     SSTIVWLVYGFSFWLWPVIIANLAICSLSLILVYFKFTFTA"
     gene            2459..2758
                     /locus_tag="VGA21_13365"
     CDS             2459..2758
                     /locus_tag="VGA21_13365"
                     /inference="COORDINATES: protein motif:HMM:NF022281.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2752 domain-containing protein"
                     /protein_id="HEX9651546.1"
                     /translation="MSFKPGSGVVIIGISIIIYAILLVLNASGIETWLPRCLLTHFTG
                     LNCLGCGMNRAIILFLQGNFVAALEMNSLIIFTIPLAIIFVYYLPSFLFKKKYYG"
     gene            2751..3539
                     /locus_tag="VGA21_13370"
     CDS             2751..3539
                     /locus_tag="VGA21_13370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651547.1"
                     /translation="MDKNVIPFPDKYEFDMGIYFSRGWQLFKLGSGSFIGFSILFIVI
                     IVVVSVTPLISVVSGLFQYLLMAGVYIFCRNLLKKKDEFSQFFRGFNYFIQILIFFLI
                     ISFFLLPLSYILTNQILPPGLLEQYMAGEVRPAEVLEVISSAIEANYNYVMLLLTLII
                     IISLYLHISYSFTLPLIADSELQFWDAMEASRKIIAQKFFHFLGMYLLLGVIIFISTV
                     ITCGFGLIVSLPFSLCVIFSAYDEIVYPPSEAKDELTNDNVTFA"
     gene            complement(3600..3989)
                     /locus_tag="VGA21_13375"
     CDS             complement(3600..3989)
                     /locus_tag="VGA21_13375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651548.1"
                     /translation="MRRFKFFLIPIVLISLAIFAVFVLMSLWNWLIPELFGLGTITFL
                     QALGLLLLLRILIGPRFGFPLFRRYAISGFGNPGRRHYWRTRMHEKWNNMNEEERAKF
                     HNYRCGSFKEKEDKVDENRDETAKSEN"
     gene            complement(4038..4364)
                     /locus_tag="VGA21_13380"
     CDS             complement(4038..4364)
                     /locus_tag="VGA21_13380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651549.1"
                     /translation="MMFRTKNRVKRITAFVVFGTIFFVAIGYLVMVLWNGLIPEIFSS
                     MPEITFWQAVGLLILTRILFTGFRPGGYRRRGAWRNKWMEMSEEEREELKRKWKERCE
                     KKETPD"
     gene            complement(4367..4942)
                     /locus_tag="VGA21_13385"
     CDS             complement(4367..4942)
                     /locus_tag="VGA21_13385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020600042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX9651550.1"
                     /translation="MEETQAISMSTDAQKNLVAAVKEYSKRLFSFIRKKVNSDEDAND
                     ILQDVWFQFSNIWYSESIEQVGAWLFTVARNKITDKYRKKKTELIDDYAYENEEGEIQ
                     FPKIMISESFSPENEQLRNLFWDELLNALDELPENQKEVFIMNELEDFTLQEIADKTG
                     AKLKTVISRKGYAVKHLRIRLQSLYEEFFNY"
     gene            complement(5021..7390)
                     /locus_tag="VGA21_13390"
     CDS             complement(5021..7390)
                     /locus_tag="VGA21_13390"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9651551.1"
                     /translation="MLKNYLLVSFRNQIRNKGYFTINLFGLTLGIVSSLYGFLWIKDE
                     LSINTFHNDMERIALVRANWQQSNGNISTFSNTQGTVYQAAKDEVAEVEHATRVSTWG
                     NSILMKSGENSFIEKGIYADKEFFEIFNFPFTEGDAQVAITQQNTIAISEELAKKYFI
                     NSDPLGKTFELNGRVATVSAIFKNVPSNSTLQFDFAMPFEDFLKDYGYEADWTNYDFY
                     TFVKLNNASDFEAADLKFESMIQNHLSEDVNEWLFLQPFNDIYLYSKFTDGKVTGGRI
                     EYVRIFSAVILFILFIACINFVNLATSRAANRSKEVGVRKVNGAHKSQLVFQFLTESF
                     LITLISLVVSVVVVELFLPGFNFLTEKSIIINYGDGLLLFGLAAIVIVVTLASGLYPA
                     FYLSGFKPALILKGSSSVAVGGITLRKFLVTFQFALSVILLISASVISNQIDFIRNTN
                     LGFDRENVVIIWSRPGISNNFESFRNKALENPEIVNLGASNQILLDVSNSTTSVNWPG
                     KTDLDDIQFRAIITGMDFLETMGIELIEGRSFNRDFPYSQDDSLKQFIITKKSAEMMK
                     LENPLGALISLWDSPGTIVGVVDDFNSKSIHSRLDPIVFMLDSRNQGRVFLKLQEGQT
                     LAGVQIIENLYKEFEQNYPFEYSFMDDSFDRQYKLESLTEKLANMFSLMAIAISCLGL
                     FGLTSFLIDRKTREIGIRKVLGASVSGLIIKLSSGFMKLFVLAVIFGSSIAYFLMINY
                     LKKFAYHMDFNFWIIGVTAGVLFLISQLTVIYHISKAALSNPAEILRNE"
     gene            7508..8794
                     /locus_tag="VGA21_13395"
     CDS             7508..8794
                     /locus_tag="VGA21_13395"
                     /inference="COORDINATES: protein motif:HMM:NF037979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HEX9651552.1"
                     /translation="MSIVKSVFSSRLTTVLTLIGVAVGLGNVWRFPYMMGSYGGSAFL
                     AIYLVFTLLLAIPALMAEMHFGKRSRNGTVGAFRHAFGNPTGTWIGYLLVTVITISAS
                     YYGIIVSSVVFSTGFSLAAGFSGKNIDAYNSLLANPSIQFAGTFILILVSLLVVDKGL
                     NKGIEWISRRIIPFFLMALIYIIIHTLTLPGAIEKLILFLKPNFSVISATEIFAALGQ
                     AFFSVGLGGTFVVVYSGYMKDNEQIPGVALYTGLGDLGSSMLISLFLVPSILIFNLDM
                     ASGPGLIFSTLPRLFEVMPGGRFVGTLFLLSISLVAFLSLVAAHEVAITSVNHEVKRI
                     SRRKIILVCGLIQSLLIIPSILNPDLIAILDLIFGSGMQVFGSLMAVIGITWFLKSKE
                     AFATKTGAGIIFLWAKWVIPIILTTILISYIYETIS"
     gene            8806..9270
                     /locus_tag="VGA21_13400"
     CDS             8806..9270
                     /locus_tag="VGA21_13400"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX9651553.1"
                     /translation="MKKNLLKQDEDHIQLDAVEDHNGILTIRGGGDKRNWNGIHYKQG
                     LSSKNVGSTNLSINIATIPPGGVAYAHVHDGFEVMLYIHQGNVRHEFGHKLQKILENG
                     AGDFIYIKSGVPHEVFNMSDTEPVVAFVARSSANEWDRIVPYDREKGEMVKD"
     gene            9512..10534
                     /locus_tag="VGA21_13405"
     CDS             9512..10534
                     /locus_tag="VGA21_13405"
                     /inference="COORDINATES: protein motif:HMM:NF040069.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6340 family protein"
                     /protein_id="HEX9651554.1"
                     /translation="MRTIFLLIFIILSSCSMRLVHMRAMQPALITVPTEVNTILLLDR
                     SSPESKEKEIIGSILSADTPGDRNAVIAQFFSGIESQFSAVNRFQTIKATESLPGTNL
                     SSAFPVQFAWEQVETLCDKYEADAIISLEVLSTRFLVTNGTRMTTKKVGEVNIPFPQI
                     YAKGVATADIGVRLYDPVLQKVVDEFLFSSTRDWEVNGVTIADAVRLLIDKTEALRDV
                     SFAAGLQYGSRLSPEPIRITREFYKKPKRNAAMERGRRQADTRLWKEAAETWENGIEN
                     SNNKTAGRLAYNVAIAYEVLGSFDLAIKWAQTSYVEFGNKKAKEYSDMLHRRLNRENI
                     LKDQLN"
     gene            complement(10538..11080)
                     /locus_tag="VGA21_13410"
     CDS             complement(10538..11080)
                     /locus_tag="VGA21_13410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651555.1"
                     /translation="MLVKRISGFIFLMLVSGVATPGHSQMLDIDRYQMMIEFLAKNGG
                     RWESIRENYDQNKVASVKGDGFYCYIDQDKNFFRIRKVFHFRNSDFTTYERTWYWHPA
                     EKKILFITWASDGRVETGSVEVVSEKAFVLTYDATDSEGNVVKRKEENFISGDREFQT
                     KVLQLKDNDWEEVERFDWKR"
     gene            complement(11091..12788)
                     /locus_tag="VGA21_13415"
     CDS             complement(11091..12788)
                     /locus_tag="VGA21_13415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651556.1"
                     /translation="MKEEIQYDVIILGGGLAGLTLSIQLKKSNPEISILVLERRATEA
                     PAAAHKVGESTVELGTHYLREILDLKSYLDKKQLPKYGLRFYLTPQHKEDLTKRVEIG
                     PRVKLPVPSHQLDRGIFENELCRITSDLGCKLILGASVKEVNFSDDFHEVLYSKSGDK
                     FSAKGKWVVDATGRAGVLKRKLGFQRPLDHDVNAVWFRYNCVIDVDDWSADPVWSGQL
                     KPGLRKLGTIHFMDHGYWVWIIPLTEGSTSIGIVADQKLHPFDSLNTHEKAIQWLEKN
                     EPQCAEKFKEKKDDLLDFMILKHYAHHSGKFYSGERWGVTGESGAFLDPFYSPGTDFI
                     SLSNCWLHDLITRYLSGEDVSVRSIIYERVHTALIDNWLPIYQNKYPLMGNTQIMVCK
                     IFWDFATYWSIQVPIFVNNGFTNIRLLTRLSSESESIFQRLGKLNNQVQNLFVAWSPY
                     EKETFTHKYFDPLEIDFLKNFQQNIGKKTATPEELLARLESNMNVLEKIASELFRRVN
                     TVVNNQPYDLVVNPYQMDLSVGPGDGPENLFGTDTLISEDIDKFWMYSFSKDPVITA"
     gene            12947..14293
                     /locus_tag="VGA21_13420"
     CDS             12947..14293
                     /locus_tag="VGA21_13420"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEX9651557.1"
                     /translation="MNSGNIILIISGLITLIACKEEIVPEAYKPTNKHKAYIFSLEEL
                     NLDKTALGSEWIKSSEEALLSPVWIETPFEEAFYLEPSQAEALSYRFSVKRGQKLMIN
                     VYSYGADSVILFMDLFRIVNDSLKQWNHVASSDDEDNKLAFEARENSDYIVRLQPELL
                     RGGKYSITIRKVPTLIFPVTGKDSRAIGSFYGDPREGGKRNHHGVDIFANRYTPIIAP
                     TKGYIRFVGERNLGGNVIWLNDVQRGQNLYFAHLQEQLVEENTWVEAGDTIGTVGNTG
                     NARSTPTHLHFGIYSRGPQDPLFFIKETNLEADSISVNQELIGLFARSMNDQLFIPNI
                     SPAFKQGSFPDTIKTHQVFKIIGGFRNYYKVQLPNQQIAYLLNRFVESISSPIDEQVI
                     RNETASLLNEPSSSGVSKETIREGEQITVLGHFSDFWWVQTSNGSLGWLPKYRVTQ"
     gene            14365..14964
                     /locus_tag="VGA21_13425"
     CDS             14365..14964
                     /locus_tag="VGA21_13425"
                     /inference="COORDINATES: protein motif:HMM:NF015639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HEX9651558.1"
                     /translation="MSAKVRSVERLFKSLDKRIHQFQSTTRLQCAPGCGKCCQKPDIE
                     ASVLEFLPFAYHFYHENDFATVMKFVEAPESRICKLFKPLVIESEKGFCGNYNNRGLI
                     CRLFGYSALRDKSGKPTMFTCSIIKSSPEYPETLAKLSDGLRIPFTADYYKRLSQIDY
                     GLANELLPVNAAIRRAIQQVVQYYTYRPKMKPGNLKKDG"
     gene            15029..15727
                     /locus_tag="VGA21_13430"
     CDS             15029..15727
                     /locus_tag="VGA21_13430"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HEX9651559.1"
                     /translation="MRILFIFTCISIFSISLYSQSKDVIARVYLNNSAKSYSAAGSSK
                     FTGTLIEALESETGSDKSLHIAELFQFFQNNELKADTVTYGKNDPGSEFIFANRDDRS
                     TNYALIIYVEEYDTWEKIEGIENDAKKIESLLADNFGFNTELVSNPTRVELLMTIRKF
                     TTMKYKPNDHLFIYFAGHGYYDELYKWNYLVLKDSQKDEEVKQSYMPVSQLLMLADNI
                     SADKVLVFIDAGAK"
     gene            15733..16167
                     /locus_tag="VGA21_13435"
     CDS             15733..16167
                     /locus_tag="VGA21_13435"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEX9651560.1"
                     /translation="MPEIHHNFVVNQGIEKVFNGVSDPIYLDKWWSKNSSGNPRTGEI
                     YKLGFGPGYAWEAEVTSVSKPDLFELKLIKADNDWMGSKVTFELVSLNDKKTQVKFHH
                     SGWQSDSDHFRISNYCWAMYLRILKRYLEYEEEVPYEKRLEV"
     gene            complement(16168..17502)
                     /locus_tag="VGA21_13440"
     CDS             complement(16168..17502)
                     /locus_tag="VGA21_13440"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEX9651561.1"
                     /translation="MNHLKNLNLVILSTAFLAFSCTNAEKNADAVMTEEEIITKAREV
                     HETVITLDTHDDINTANFTLDNNYTMDLDTQVNLVKMDSGGLDVAWFIVYTGQGDLNE
                     QGYAAAYENAIDKFNAIHLLAEAIAPDKVEIALTSHDVRRIVESGKKVAMIGIENGYP
                     IGTNISRVEEFYNLGGRYMSLAHNGHSQLSDSNTGEADSVWLHNGLSELGKEVIAEMN
                     RLGMMIDVSHPGKEAMKQMVALSKAPIIASHSAARALCNHSRNLDDEQLMLIKKNGGV
                     VQTVAFASYVSEGKTKKRDSLIQIINKEVAAEMGFELLGSFREVRQLPEADRNTYIAK
                     FREMRTKAEPRIQSEVDPVAPRVNVSDFADHIDYIVNLIGLDHVGISSDFDGGGGVEG
                     WNNAAETFNVTLELVRRGYTQEEIAKLWSGNLLRVLDEVQAIAGQIQSETSQ"
     gene            17764..18318
                     /locus_tag="VGA21_13445"
     CDS             17764..18318
                     /locus_tag="VGA21_13445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651562.1"
                     /translation="MRKVKVLIILIPALVFSGAPLLQAQDIYASVHNGFGVSFVDAGN
                     RLLTGQSLSNTNRFSWFGSIEGLYLLPSGYLLGVEFGAHRLYSYKWYDGVGNRTTTIV
                     TYHPGAIVGIVLKDNFYIKAGVNLRAFSYGGVSPGAMIALDYALPLNDLFSIPLGLRS
                     DIIIDGNIILSLNASIGLRYYINY"
     gene            complement(18492..18854)
                     /locus_tag="VGA21_13450"
     CDS             complement(18492..18854)
                     /locus_tag="VGA21_13450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651563.1"
                     /translation="MAKQKKAGKSARKKAAPKKKATRKKSAAKKAAPKKKAAKKKAAP
                     KRKAAKKSSAKKKAAPKKKAAKKKAAPKRKKKAAAKKSSAKKAAPKRKAAKKKAAPKK
                     KAAKKKAAPKKKAAKKKK"
     gene            complement(19186..19605)
                     /locus_tag="VGA21_13455"
     CDS             complement(19186..19605)
                     /locus_tag="VGA21_13455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008623971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEX9651564.1"
                     /translation="MKYWIRTKIESSKSHLALLIFRVAVGGMMLTHGIPKARRVFAGD
                     WRFGDPIGLGSEVSLLLATFAEAGCGILIILGLWTRLASVPLVITMAVAGFIHHAPDP
                     FSTKEKSFLYLAAFILLFFLGGGKYSLDRTLSKPDNY"
     gene            complement(19671..20117)
                     /locus_tag="VGA21_13460"
     CDS             complement(19671..20117)
                     /locus_tag="VGA21_13460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651565.1"
                     /translation="MWKIIPLLILISTEALGQEAPKKATTIKVITTDKLEEVFQKYEK
                     YLDDEGYIIRNKENLISLQTSLKEKSLGPMSSYSVYVEYLLEFKQMEDKTSVTITGNY
                     ADNGGMSKSKAITNKGSSNFGVGLAWRIMNELAIRYPGGQISYSME"
     gene            complement(20172..21287)
                     /locus_tag="VGA21_13465"
     CDS             complement(20172..21287)
                     /locus_tag="VGA21_13465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651566.1"
                     /translation="MVKKLIRITRLCFLCNLVFFCFISFHSCKIQSSNEGVEVEVIYE
                     SGKAAAVKIHSKLLEDRDASDDLKIELENNESNTNVLGEFYWNENILFKPLIPLTPGK
                     TYLIMAGGEAIAKFTIPESDNSSVPGITEIFPGQDTVPSNLLKMYLQFSEPMGVDHSG
                     KFVKVLDAKGDSVSPIFLNLVPELWNEDKTILTLWLDPGRIKRGLIPNERLGAPLIEE
                     RYYTLIIMPGWRSKKGTGTGEEFNKKFFVSHRDSISPSTDSWTLLLPLAESNDRLVIH
                     FNETLDYILCQEAISIWKDTVRISGKATLTNEEQQFNFYPDSPWKEGDYKVEAESRLE
                     DLAGNNLNRLFETDKTQAPPVHSAGSRYLHFIISDHK"
     gene            complement(21281..22381)
                     /locus_tag="VGA21_13470"
     CDS             complement(21281..22381)
                     /locus_tag="VGA21_13470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651567.1"
                     /translation="MKKYYVNGTLVLVLIVLFAGANKTADLFEGFETGTPEIKSMSSL
                     TFGPEGILFIGDTRNAAVFAVETGDTSPGEAPQRINISNFDRLVAESLGTDVKNIDIQ
                     DMAVNPISKKLYFSVHTNDGSAVLLKLEGEKLAAVKLSDIRYSTTGLHNAPGEGEVDD
                     RGRSKRIWSISDLNYFNDQVMITGISNQEFGSSFRSIPFPFQDSQSMSSLEIYHGNHG
                     RYETNSPVRTFIPTEIKGEKYIVASYTCTPLVLFPLNELKPGKHVKGRTVGEFGYGNA
                     PLDMIIMPKDGKNYLVMNNSNRPVMIIDMDKLASYEGALTEPVEYIAGVEHTTMPWVN
                     VLQLDKVNDNQFVMLQRRSNGNLDLWSASPRW"
     gene            22613..23290
                     /locus_tag="VGA21_13475"
     CDS             22613..23290
                     /locus_tag="VGA21_13475"
                     /inference="COORDINATES: protein motif:HMM:NF027591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4919 domain-containing protein"
                     /protein_id="HEX9651568.1"
                     /translation="MKVRLIAFFLVLSISVTAQFQAPNYDGISKVIKDKASPYFYPEL
                     LSRYHDNDTALSTQHYRILYYGYIYHENYDPYSLPLQFDSLNNILKKEIFTPNDYQEL
                     IEMEKRILMKFPFNLREVSLLAKAYEETGQMELAAAMHDKFRKITEAILSTGDGKTKE
                     TAMHVIMNSHEYDILHYLGLEYNNETAQTESTYELLSVKPNKYNLEGIFFNIKKMFEH
                     EAKILEK"
     gene            23454..25877
                     /locus_tag="VGA21_13480"
     CDS             23454..25877
                     /locus_tag="VGA21_13480"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9651569.1"
                     /translation="MYKSNLIVAWRNLIRNLLHTSINIFGLTIGITACFSIFTLIRYE
                     YSFNKNHPDLDRIYRIYSVYGGAFSGVNRGVCGALPVYIKENFSGLENVANFHTWSAK
                     VEIPLNDGSVKDLDTQSDLVIAVPDYIDLVGQYNWLTGSKAILGVPNKVVLIESQAKK
                     YFGELPYDELLGRQIIYSDSLFMEIGGVVSNTKDNTDFAFTDILSFATIKSTWLKDNY
                     ADNDWGSTNSSSQLFVKLSEGTLIDNLNEQLESAAEKGDAETDSEWTTTFSAQPLSDI
                     HYNTEIGVFDNVSHVANLDTLRKLTWIAIALLVIAAINFINLETALATKRSHEVGIRK
                     VLGSSRVRLVFHFLNESFMITTAAVALSIPLIMASISIFKDYIPEGVSFNLSDTTTLI
                     YLGLTIAIVGLLAGLYPAFVMSSFSPVKSLKNQSGRKNSLSANLRKGLTIFQFGFSQI
                     LIIGTVIVGLQIHYMHSKDLGFETKSILYFYTSYYAELSKKDVLKNELQRIPEISSLS
                     NFGNPPAQRGYSTSITKFINDKGDEIMSNAHQKSGDTTYIPFFGLKFAAGRNYRPNAA
                     GKETVINETFARELGFEDSFDALGNIFRRGNDTLTIVGVLEDFHFRSLHHKIEPIMIT
                     LDNTARSFGIKMTESANTDQVIEKIKNEYAKVYPDRTFAHYFLDETVEQFYKTEQRAS
                     KLAITATAIAIIISCLGLFGLASFTAVQRTKEIGIRKVLGATADMIVSLLSKDFLKLV
                     LISIVISVPIAYWIGELWLEDFAYKIPISGWIFVLSGLGSILIAFLTVSYQSIKAAMA
                     DPAESLRYE"
     gene            complement(25880..27757)
                     /locus_tag="VGA21_13485"
     CDS             complement(25880..27757)
                     /locus_tag="VGA21_13485"
                     /inference="COORDINATES: protein motif:HMM:NF024245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEX9651570.1"
                     /translation="MIKVISASLLVFVSASLLAQSEKSFVLIQPVYDGKGEMPLMELV
                     PDTANIYKVSQKALEESFIGQAIQLYHFAQNYLINTGQVSAREPAYLAITKNQGGFAK
                     KGFQIKTLEGTINKSNAYYVDLTANSITSKLDRVMSVSQLYPHELGHVIFRLLSSTDS
                     IEQQAKSVTVHYFSLMTDNSVAFNEGFAEHMENISRIYERNETIRKGIENDVAVREPN
                     LLRASESFVRDFNWPLRLDYYKCTMLVWYQQYEDFKRYKHSLEGTVTFQNGYPALSSV
                     QDMMSVRNAGVIHLDNKRNKAQLMCSEGFVSAFFSRLAVNGLGKYYRNAKFYSNFLID
                     TIGVIDPVQKFNEVENLFVKYFHVLNKHVRLEQSDRAQLMDFIEGYMEEFPDESEIIL
                     STYQELSGEAYSPDYPVEIWLMVKNYNHRVLALDALGAITLPVYTFNLNAAQGVDLLS
                     VPGMDGQSAAELIRYRDEIGPFQSLNQVLELSHVTEEAKKGLRNSEFDEAYLEGMDEK
                     SLSIGPLLSVPLFHLLKTGTFAYLIIFSATFLLLLRKTSKGLNALLTFIKYYFIWILL
                     LVAGLAALVVQGNPILLFLPIMVVYYIFGYLILRKKYRSLKHFILISFLMHLVIIYSL
                     I"
     gene            complement(27845..>28915)
                     /locus_tag="VGA21_13490"
     CDS             complement(27845..>28915)
                     /locus_tag="VGA21_13490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009582652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline racemase family protein"
                     /protein_id="HEX9651571.1"
                     /translation="IKKFAMSSTFQRIQKQQFFQNKNDWISIRTVDMHTGGEPLRVIL
                     SGLPTLEGKSVLQYRRYMKENLDKYRKLLMLEPRGHADMYGCVLLPPNNDSSDFGILF
                     LHNEGYSTMCGHAIIAITKLAIEQAWINMEEPVTRMKIDAPCGTIESFARIQNGKTRS
                     IYFHGVPSFVVGVNIPLNTASIGAITYDLAYGGAFYAYVDADKFEIDLSQENYTGLIH
                     LGMEIKKEVIRTNKHIKHPIEPDLSFLYGTIFISNKSLTADSKNVCIFANGEVDRSPT
                     GSGVSGRMALHYKKGDLHIGEEMKIESITGSIFTASVVELVKFGEFDAVIPRVEGTAF
                     ITGQHEFLLDPEDPFQEGFFLR"
BASE COUNT         8687 a         5837 c         5446 g         8945 t
ORIGIN      
        1 ccaggaatat gtgggtcatg ataataaccg ggattggttt atgagcaaca tgcctgagac
       61 aaaagcagcc acaaccgtat tgtataatca atggtatcca caaattgtgc ataatcacca
      121 ccagtcttct ccggcgtggg cccgaatttt tatccctcct ttccgcagcc cggttaatga
      181 taaaatacat ccaggtgtaa caaccggtgt aaatatggtg ggtgcagcaa tggcaagcag
      241 gtttgtttcc aagaaaatgc cgggagcaat ttcgcaaacc acttatgaga tgtggtggaa
      301 tggcggtatg cggacggttc cctactatca taatatgatt ggtatcttaa cggaaaccgc
      361 ccacaccact cctactccga gatactatcc tccggactca attcccaaaa tggttggggg
      421 gaaaactcct aatggaactg aaatttttta tccatatccc tggaaaggtg gtgaatccca
      481 tttccgggat ccgattaatt atatgattga agcgtcaatg gcgatacttg atcttgcgtc
      541 ttcaaggaag gaatattttc tgaaagggat ttatgacatg ggtcgcgatg caattgaaga
      601 tgaagccgga gcttatgcct atgttatacc aaaaaatcag tgggatgcag gtgaagcaaa
      661 taatttggta aacatactta tgcagggcgg aattgaagcg agtcaggcaa cttcaaaatt
      721 cactgccggt ggccaggaat atgaagccgg gagttacgtg ctttatggag cccaggcatt
      781 cagaccttat cttgcagatt tattggaaaa gcaaaattac ccggaattat atcaatatcc
      841 aggcgggccg ccaatctctc cctatgattt gaccggatgg actttgccta tgcaaatggg
      901 agttaaagtt gatcgaatcg atcaatcatt ttctgctaac actagagtcc tcactcaaca
      961 aattgacgtc accgccggca aaattgatgg ctcagcaacc tatggatacg cattgagcaa
     1021 caacgagaac agaagctata aagctgttaa ccgtctgata aatgccggtg agaaagtttt
     1081 aatcagcgac caaccatttt ctgatggcaa gagtaatttt attgcaggca catttattat
     1141 tgagaagggt aataataccg aaaatcttgt aaaaacatta acgcgagagt taggcgttac
     1201 cattgccggt atcaaaacga agccttctgg gaatttacgg aaccttggaa aggttaaggt
     1261 cggcgtttat aaatcatggc aggccaacat ggatgaaggt tggacgcggt ttgttttaga
     1321 acaatatgat tttgatctgg acacccttca taatgaagat attctctcga aaaacttatc
     1381 tgcttattcc gctatcatat tccctgctca aagccccaat ggtataatga atgggcatga
     1441 accgggttct atgcctgaga aatacgtggg aggtattgga attgaaggac ttctggctat
     1501 tgagaatttc accaagcaag gaggagtttt gataatgttt gatgatgctt ccgatcttgc
     1561 aatagagcaa tttggacttc cggtggatga tatcaccagt aatttgtctt ccagtcagtt
     1621 tttcattccg ggatctattg taagaattaa gaatgattta aataaccctc ttgcttatgg
     1681 aatgcaggag gaaaccgggg cttcttttca aagaagtaaa gcattcaatg ttgtttcaca
     1741 gcggcgatat gcagaaggag gccgtgaaga aacaaaacct gcagagaaac cggatgtaga
     1801 aattgtaacc cggtatgcaa gttccgagat acttatgagt ggttggggca atggagtgaa
     1861 cagattaaaa aatatgggtg ccgtaatgaa tgttggttat ggcaatggga gaataattct
     1921 ttttggattc cgacctcaat tccgtgccca gccaagaggt acttataagt tgatttttaa
     1981 ctcaatttat ttaggtgctt caaaacaagc aaatggcaat aataaatcag aataacgtct
     2041 aaatatcaac cggaattaat gcgtggctga attgttacct attttttatg ccgtgaaagt
     2101 aaatttaaaa tagaccagaa tcaggcttaa agaacaaata gccaggtttg caataattac
     2161 aggccacagc caaaaggaaa atccataaac cagccatact atagtgctac tgaaaacaat
     2221 aagaatcatt gtcatgctta aatcctttac actttttgtt ttccaggctt tgtaaacctg
     2281 tggtatgaat gtaatactac taagtagcgc tccgaaatga cctactagtt cagtccaatg
     2341 cataatcttt taaagttttg gaagaaaatt gagccaagat aaggctaaat cctaaaatct
     2401 gaattcaatg cgtaatttta atattgattt agatgtgata aaggaatagt tttatacaat
     2461 gagttttaag ccgggaagcg gagtggttat aattggcata tcaattatca tttatgccat
     2521 tttgcttgtc ttgaatgctt cgggaattga aacctggctt ccgcgatgtt tgcttactca
     2581 ttttaccgga ttaaattgtt tgggctgtgg aatgaacagg gcaattattc tgtttctgca
     2641 aggaaacttt gtggctgctc tcgagatgaa ctcactgatt atttttacca ttccattggc
     2701 cattattttt gtttactatc tgccttcatt cctgtttaag aaaaaatatt atggataaaa
     2761 acgttattcc ctttccggac aaatatgaat ttgacatggg catatatttc agcagaggct
     2821 ggcagctttt taaacttggt tcgggaagtt ttatcggctt ttcaattcta ttcattgtaa
     2881 tcatagtagt agtatctgtt actcccttga tcagtgtagt cagtggcctt tttcaatatc
     2941 ttttaatggc aggagtttat atattttgcc gcaatttgct gaaaaagaaa gatgaattct
     3001 cacaattttt ccgtggattt aattacttca ttcagatcct aatattcttc cttattataa
     3061 gttttttcct cttaccactt tcctacatcc tcacaaacca gatcttacca cccggcttac
     3121 ttgaacaata tatggcaggc gaagtcagac cggctgaagt tcttgaggtt atatcatctg
     3181 ccatcgaagc taattacaac tatgtaatgc ttttgctgac attaatcatt atcatttcac
     3241 tgtacctgca tatctcctac tcattcacac ttccattgat tgcagattct gaacttcagt
     3301 tttgggatgc aatggaagcc agcagaaaga ttattgccca aaaatttttt catttcctgg
     3361 gtatgtatct actgctggga gttattattt ttataagtac ggtaataacg tgcggttttg
     3421 gactaatagt aagtttacca ttttcccttt gcgttatttt ttccgcttat gatgagatcg
     3481 tttacccgcc atctgaggca aaagatgagc ttactaatga taatgtaact ttcgcctgat
     3541 catagaaaaa gccgcaatga aagaacattg cagcttcaca aagaattaga gaactctaat
     3601 taattttctg atttggcggt ttcatcccta ttttcatcta ctttatcttc tttttcttta
     3661 aaagatccgc accggtaatt atggaatttg gctcgctctt cttcattcat gttattccac
     3721 ttttcatgca ttcttgttct ccagtaatgt cgtctgcctg gattgccaaa tccggaaatg
     3781 gcgtatcttc tgaaaagcgg aaaaccgaat cgaggaccga tcaaaattct aagcaacagt
     3841 aacaagccaa gcgcctgtaa aaatgtaatt gtacccaatc cgaaaagctc cggtattaac
     3901 caattccata atgacatcaa cacgaagact gcaaaaattg ccaacgaaat caacactata
     3961 ggaatcagga aaaatttaaa tcttctcatt gtcgtaatta ttatttataa ggaagacgat
     4021 gaactttcag aattacttta atcgggtgtc tcttttttct cacatcgctc cttccatttt
     4081 cttttcagct cctcgcgttc ttcttccgac atttccatcc atttatttct ccaggctcca
     4141 cgtctccggt aacctccggg acgaaatcct gtaaaaagaa tcctggtaag aataagaagg
     4201 cccacagcct gccaaaaagt tatttccggc atggaagaaa aaatctccgg aatcaagcca
     4261 ttccaaagaa ccattaccag gtagccaatc gctacaaaaa agatagttcc gaaaaccacg
     4321 aatgccgtaa ttctcttaac ccgattttta gtcctgaaca tcattttcaa taattaaaaa
     4381 attcttcata taatgattgt aaccgtattc gtaaatgttt tacagcatac ccttttcggg
     4441 aaatcactgt ttttaatttc gcaccggttt tatctgcaat ttcttgtaat gtaaaatctt
     4501 caagctcgtt cataatgaat acttcttttt gattctctgg taattcatcc aatgcattta
     4561 gtaattcatc ccaaaataaa tttcttaact gttcattttc aggagagaaa ctttccgaga
     4621 tcattatttt gggaaactga atttcacctt cctcattttc gtaagcataa tcatcgatga
     4681 gttcagtctt cttcttcctg tatttatctg ttattttatt cctggccacg gtaaataacc
     4741 atgctccaac ctgttcaatc gactcactgt accaaatgtt gctaaattga aaccagacat
     4801 cctgaagaat atcattcgcg tcctcatcag aattaacttt cttacggata aagctaaaaa
     4861 ggcgcttact gtattcctta actgctgcaa ccaggttttt ctgtgcgtct gttgacatgg
     4921 aaatggcttg agtctcttcc attatactaa agacgactca aaccaaaatt tactctatct
     4981 gattaagaaa atctcaattc caaaatcaaa atagctgaaa ttattcattc ctaagtattt
     5041 cggcggggtt tgacagggcg gctttactaa tgtgatagat aaccgttaat tgagaaatca
     5101 ggaataaaac gcctgctgtt actccaatta tccaaaaatt aaaatccatg tggtatgcaa
     5161 actttttaag atagtttatc atcaggaaat aagcgatgga actgccaaag atcactgcca
     5221 gcacaaacaa tttcatgaag ccggaagata gtttaataat tagtcctgac accgaagcac
     5281 ccaggacttt ccgaattcca atttcacggg tttttctgtc aatgaggaaa gaagtaagtc
     5341 caaacaatcc cagacaggag atggcaatag ccatgaggga gaacatattt gccagctttt
     5401 ctgtcagaga ttcaagttta tattgcctgt caaacgaatc atccataaag gagtactcaa
     5461 atggataatt ctgctcaaat tctttataaa gattttctat gatttgtacg ccggccagtg
     5521 tctggccttc ctggagtttc agaaataccc ttccctggtt cctgctatca agcatgaata
     5581 ctattgggtc tagccgggaa tggatgcttt tactgttgaa gtcgtcaaca actccaacaa
     5641 tagttcccgg ggaatcccaa agtgaaatca gggctcccag cggattctca agtttcatca
     5701 tttctgcact tttttttgta atgatgaatt gttttagtga atcatcctga gaatatggaa
     5761 aatccctgtt gaaagacctt ccttcaatca attcaattcc catcgtttca agaaaatcca
     5821 ttccagtgat tattgccctg aattgaatat catccagatc tgtttttcca ggccagttta
     5881 ctgatgttgt tgagttacta acatcaagca gaatctggtt tgatgctcca agatttacaa
     5941 tttccggatt ttccaaagct ttatttctga aagattcgaa attgttgcta atgccaggtc
     6001 ttgaccaaat gattaccaca ttctcccggt caaatcctag gttagtattt ctgataaaat
     6061 caatctgatt gctgatcacg gaagcactga ttagaaggat cacggataaa gcaaattgaa
     6121 aggttactaa aaatttacga agcgtaattc cacctacggc aactgatgac gatcccttta
     6181 atatcaatgc cggcttgaaa cccgaaagat aaaacgcagg ataaaggcct gatgccagcg
     6241 tcactacaat cacaatagcg gctaatccga aaagcaataa gccatccccg taatttataa
     6301 ttattgattt ttcagtaaga aaattaaatc cgggcaagaa cagttcaact actactaccg
     6361 aaacaaccaa tgaaattagc gttatcagaa aagattctgt aagaaactga aaaaccagct
     6421 gagacttatg agcgccattt actttcctca ctcctacttc tttagaccta ttggcagccc
     6481 tggatgtagc caggttaacg aaattaatgc aagcgataaa cagaataaaa agaataaccg
     6541 cactaaaaat ccgaacgtat tctattcgtc ctccggtaac ctttccatca gtgaatttcg
     6601 aatataagta tatgtcatta aatggttgaa ggaacaacca ctcattaaca tcttcggata
     6661 aatggttctg aatcatggat tcaaacttta aatcggctgc ttcgaagtcg cttgcattat
     6721 taagtttaac aaacgtatag aaatcataat ttgtccaatc tgcttcgtat ccatagtctt
     6781 ttaaaaagtc ctcaaaaggc atcgcaaagt caaactgaag agtggagtta gatggaacat
     6841 tcttaaaaat tgcgcttacc gttgcaaccc ggccattgag ttcaaacgtc tttcctaaag
     6901 gatctgaatt aataaaatat ttctttgcta actcttctga gattgcaatg gtattttgtt
     6961 gtgttatggc aacctgggcg tctccttccg taaaggggaa attaaatatt tcaaaaaatt
     7021 ccttgtccgc gtaaattccc ttttcaatga atgaattctc cccagacttc atcagaatag
     7081 aatttcccca ggtggaaacc cttgtggcgt gttcaacttc agctacctca tcttttgctg
     7141 cctgatacac tgttccctgt gtgttagaaa aagtagaaat atttccgttg ctctgttgcc
     7201 aatttgccct taccagggca atgcgttcca tatcattatg aaaagtattt attgataatt
     7261 cgtctttgat ccacagaaat ccataaagac tgctaacaat tccaagggta agaccaaaaa
     7321 gattgatagt gaaatagcct ttgttacgaa tttgatttct aaatgaaacc agcaggtaat
     7381 tttttagcat aaggtcaagt attatatccg gttaagatgt attttccttc atatatacat
     7441 ccatttatag caaatggttg ctaagaaacc tctaaaataa cgacattggc gtaacctaat
     7501 ttgtttaatg tcaatagtaa aatcagtctt ttcatctcga cttaccactg ttttaaccct
     7561 aattggagta gctgttgggc tgggcaatgt atggcgattc ccatacatga tgggaagtta
     7621 cggaggcagc gcttttttgg ctatttacct ggtatttaca ctccttttgg ccattccagc
     7681 attgatggcc gagatgcact ttggaaaacg aagcagaaat ggcacagtgg gagccttcag
     7741 acatgcattc ggaaatccaa cgggtacatg gattggttac ttattggtga ccgtcattac
     7801 catttccgct tcctactatg gaattatagt aagtagtgtc gttttcagca cagggttttc
     7861 tttagcggct gggttctccg gcaaaaatat tgacgcatat aattctctgc tggccaatcc
     7921 ttcaatccaa tttgccggaa cctttattct tattcttgtt tcattattgg tagtcgataa
     7981 aggtctgaac aaaggaatcg aatggattag ccggaggatt atacccttct ttctgatggc
     8041 actaatttac ataatcattc atacgttaac ccttcctggt gctattgaga agttaatcct
     8101 tttcctcaag ccaaacttca gcgttatttc agccacggaa atttttgccg ccctcggcca
     8161 ggcttttttt tctgtaggtc tgggcggaac atttgtcgta gtttattcag gatacatgaa
     8221 ggataatgaa cagattccgg gggttgcttt atatacaggt ttgggagatt taggatcatc
     8281 catgctaatc agtttgttcc ttgtgccgtc aattttgatt tttaatcttg atatggcttc
     8341 tgggccgggc cttatttttt caacattacc caggttattt gaagttatgc ccggcggcag
     8401 attcgttggt acgcttttcc tgctttcgat ttccttggtt gcatttcttt ctcttgttgc
     8461 agcccatgaa gttgctatta ccagcgtaaa tcatgaagtt aaaagaatct ccaggaggaa
     8521 aatcattttg gtttgcggcc ttattcaatc ccttttaatc atacctagta ttttaaatcc
     8581 tgatttgatt gcgatacttg atttaatttt tggttccggg atgcaggtat ttggaagttt
     8641 gatggcggta attggcatca cctggttttt aaaatcgaaa gaagcttttg caacaaaaac
     8701 cggagctgga attattttcc tctgggcaaa atgggtaatt ccaataattc ttactacaat
     8761 attaatcagt tatatttatg agactatttc ctaattaaaa ttcatatgaa aaagaatctg
     8821 ctaaaacaag atgaagatca tattcaactt gatgctgttg aagatcataa tggtatcctg
     8881 accattcgtg gtggcggaga caaaagaaat tggaatggca tccactataa gcaaggactg
     8941 tcttcaaaaa atgttggttc cacaaattta tccattaaca tagctacaat acccccggga
     9001 ggagtggctt atgcccacgt tcatgatggt tttgaggtaa tgctttatat tcaccaggga
     9061 aatgttcggc atgaattcgg ccacaaattg caaaagattc ttgaaaacgg cgcaggcgat
     9121 tttatctaca tcaaatccgg agttcctcat gaagtcttta atatgagtga cactgagccg
     9181 gtggttgcat ttgttgcccg ctcaagtgcc aatgagtggg acaggattgt tccatatgat
     9241 agagagaagg gtgaaatggt aaaggattaa tatcgaaata cctatatgaa gagttagcag
     9301 gcaaggaata ctttggattt actgccacac ttatgacaga tagtagtctg aatcttgttc
     9361 gcccgcttcc attttgagtt aatcatcgaa aacaaaaatt aaattagtgc caaaatggca
     9421 aaactatcct gctcaccatg actttttctg tgggttatta cttaataaat taactaactt
     9481 ggctaaagtt tatgtctatt aattaacatt catgcgcaca atttttcttc tgattttcat
     9541 cattttatca tcttgctcca tgcggcttgt gcacatgaga gccatgcaac ccgcattgat
     9601 cacggttcca actgaagtaa acacaatatt actgcttgac cggtcaagcc cggagtcgaa
     9661 agagaaggaa ataattggct caattttgtc tgcggataca cccggtgaca ggaatgcagt
     9721 gattgcacag tttttttctg ggattgaatc tcaattttca gccgttaacc ggtttcaaac
     9781 aatcaaagcc acagagtcat tacccgggac aaatctttct tctgcattcc cggtccagtt
     9841 cgcgtgggag caggttgaaa cactttgcga taaatatgaa gccgatgcca tcatttcact
     9901 ggaagtatta agcacaagat tcctggtcac caatggaacg aggatgacca ccaaaaaagt
     9961 gggagaagta aatataccct tcccccaaat ttatgctaaa ggagttgcaa ctgcagacat
    10021 tggagttcga ttatatgatc ctgttctgca aaaagttgta gatgaatttt tattctcctc
    10081 caccagagat tgggaagtaa atggtgtaac cattgctgat gcagttcgcc tgttaattga
    10141 taagacggaa gccctgcgtg atgtaagttt cgcggccggt ctgcagtatg gttcaaggct
    10201 ttccccggaa cctatccgga taacccgtga attttataag aaacccaagc ggaacgcggc
    10261 catggagcgt ggacgcagac aagcggatac caggctttgg aaagaagcag ctgagacctg
    10321 ggaaaatgga attgagaatt cgaataataa gacagccggt cggctggctt ataatgtcgc
    10381 gatagcttat gaagtgttgg gatcttttga tttggcgata aagtgggcgc agacgtctta
    10441 tgtagaattt ggaaacaaaa aggcaaaaga atactccgat atgctgcatc gaagattaaa
    10501 ccgcgagaat attctgaaag atcaacttaa ttaatagcta cctcttccaa tcaaatcgtt
    10561 caacttcctc ccaatcattg tcttttaact gcaaaacttt agtttgaaat tcgcgatcgc
    10621 ctgatatgaa attttcctct tttctcttca caacatttcc ctcagaatca gtggcatcat
    10681 aggtaagaac gaatgctttt tctgaaacaa cttcaacaga tcctgtttca actctgccat
    10741 cactggccca ggtaataaat agaatttttt tttccgcggg gtgccagtac caggtacgtt
    10801 cataggtagt aaaatctgaa ttcctgaaat gaaagacttt gcgaatgcga aagaaattct
    10861 tatcctggtc aatataacag taaaaaccat ctcctttcac agaggctact ttattctggt
    10921 cataattctc cctgatactc tcccatcgac cgccattctt tgccagaaat tcaatcatca
    10981 tttgatagcg atcaatatca agcatttgtg aatgaccagg cgtggccact ccgctcacaa
    11041 gcataagaaa aataaaacct gaaattcttt ttacaagcaa aatctataag ttaggcggta
    11101 ataacaggat ccttcgaaaa ggaatacatc cagaatttat caatatcttc acttattaat
    11161 gtatccgtac caaataaatt ttcaggtcca tcgcctggtc cgacggacag gtccatttgg
    11221 tacggattaa ctaccaaatc atagggctga ttatttacaa cggtattcac tcttctgaat
    11281 aattcactgg caattttttc caaaacgttc atgttacttt ccagccgggc caggagttct
    11341 tccggagttg cagtcttctt tccaatattt tgctgaaaat ttttaagaaa atcaatttca
    11401 agaggatcaa agtatttgtg tgtaaaggtt tctttttcat aaggagacca ggcaacaaac
    11461 agattttgaa cctggttatt caatttacct aaccgttgga aaatggattc agactcagaa
    11521 gatagcctgg ttagcaatct gatattcgta aatccgttat taacaaagat gggaacctgg
    11581 atcgaccaat aagtcgcgaa atcccaaaag attttacata ccataatttg ggtgttaccc
    11641 atcaatggat atttattctg atagatgggc agccagttgt caataagtgc tgtatgaacc
    11701 cgctcataga taattgaacg aacacttaca tcttcaccgc ttaaatatct ggtaatcaga
    11761 tcgtgcagcc agcaatttga aagtgaaata aagtctgttc ccggcgaata aaatggatcc
    11821 aggaacgcgc cggattctcc ggttactccc caacgttcac cggaataaaa ttttccactg
    11881 tgatgagcat aatgttttaa tatcatgaag tccagcaaat catctttttt ctctttgaac
    11941 ttttcagcac attgcggctc atttttttcg agccactgta ttgccttttc atgagtgttt
    12001 agcgaatcaa acggatgaag cttttgatcg gccacaatgc caatactggt ggacccttcc
    12061 gttaagggaa ttatccatac ccaatacccg tgatccataa aatgtatggt acccagtttt
    12121 cgaaggccgg gtttaagttg ccctgaccaa accggatcag ctgaccaatc atcaacatca
    12181 ataacacagt tatatctgaa ccaaaccgcg tttacatcat ggtcaagagg tctttgaaat
    12241 cccaatttcc ttttcagaac tcctgctcta ccggtagcgt caaccaccca tttgcctttg
    12301 gccgaaaatt tatcaccgct ctttgagtat aatacttcgt gaaaatcatc cgaaaaattc
    12361 acctctttta ctgatgcccc gagaatcagt ttgcaaccca agtcagaagt tattctgcat
    12421 aattcatttt caaaaatacc cctatccaat tgatgactgg gaaccggtaa ctttactctc
    12481 ggaccaattt caacccgctt tgtcagatct tccttatgct gcggcgtgag ataaaatctc
    12541 aacccatact ttggcagttg ttttttgtca aggtagctct tcaaatctaa aatctccctg
    12601 agataatggg tgcctaattc aacggtagat tccccaactt tgtgcgcagc cgccggagct
    12661 tcagtagccc gtcgctccaa tactaatatt gaaatttcag gatttgattt ctttaattga
    12721 atggaaaggg taagtccggc aagcccacca cccaaaataa tcacatcata ttgaatttcc
    12781 tctttcatct ctggtaacta taggtatttc taagctgtgc aactaaatgt tgtaacagca
    12841 aagtaaatat tttattgtga taactcaatc ttgctcagaa ttaataatta aatcaaaaag
    12901 tgataagttt cgtataagaa tttagcccag ctaaacccgt ttgcaagtga attcaggaaa
    12961 tatcatacta ataattagcg gattaattac acttatcgcg tgtaaggaag agattgttcc
    13021 tgaagcatac aaacccacaa acaagcataa ggcctacatt ttttcactag aggaattaaa
    13081 tcttgacaag acggctcttg gaagtgagtg gatcaaaagt tcggaagaag ctttacttag
    13141 ccctgtttgg attgagacac catttgaaga agctttttac ctggagccat cacaggcgga
    13201 ggcattgagt tatagatttt cggtaaagcg gggacaaaaa ttaatgatta atgtttactc
    13261 ttatggagct gattccgtca tcttgttcat ggatttattc agaattgtta acgactccct
    13321 gaagcaatgg aatcatgtag cctcatcgga tgatgaagat aacaaacttg catttgaagc
    13381 aagagaaaat tccgattata ttgtcagatt gcagccggaa ttacttagag gtggaaagta
    13441 ctccatcaca atccgaaaag ttccgactct catttttccc gttaccggga aagatagccg
    13501 cgccataggt agtttttacg gcgaccctcg tgaaggtggt aagcgtaatc atcacggtgt
    13561 tgatatattt gccaatagat atacacccat tattgcaccg acaaagggtt atatcagatt
    13621 tgtgggtgaa cgcaatttag gtggaaatgt tatttggctt aacgacgtgc aaagaggtca
    13681 aaatttatat tttgcgcatc tgcaggaaca actcgttgaa gaaaatactt gggttgaagc
    13741 cggcgatacc atcggtacag tagggaatac aggaaatgca aggagtactc ccactcatct
    13801 tcattttgga atatacagcc gtgggcccca ggatccgtta ttttttatta aagaaacaaa
    13861 ccttgaagcg gattctattt ctgtaaatca ggaattgatc ggcttgtttg caagatcaat
    13921 gaatgaccaa ttatttatcc caaacatttc tccggctttc aaacaaggga gttttccaga
    13981 taccatcaaa actcaccagg tattcaaaat tattggtggt ttcaggaatt actataaagt
    14041 acaattacct aatcagcaaa tagcttattt acttaataga tttgttgagt caattagttc
    14101 tccaattgat gaacaggtta tcagaaatga aactgccagt ctcctaaatg aaccttcttc
    14161 ctcaggcgtt tcaaaagaaa ctatccggga aggagaacaa atcaccgttt taggccactt
    14221 cagtgatttt tggtgggttc aaacttccaa tggcagcctg ggctggctcc ctaaatatcg
    14281 ggtcacccaa taattcgctg ataccggcaa aactgatccg gattttgatt agaattgcag
    14341 acttttttct gtggttttat gacaatgtca gcaaaggtaa ggtccgttga aaggcttttt
    14401 aagtcattgg ataaacggat ccatcaattc caaagtacaa cccggttgca atgtgccccg
    14461 ggctgtggta aatgttgcca gaaaccagat atagaggcca gcgtattgga gttcctgccc
    14521 tttgcctatc atttttatca tgaaaatgat tttgcaactg tgatgaagtt tgtagaagca
    14581 ccggaaagca gaatctgcaa gctttttaaa ccgctggtta ttgaatctga aaagggtttt
    14641 tgtggcaatt acaataacag gggtttaata tgcaggctct ttggttattc tgcccttcgt
    14701 gataaaagcg gcaaaccaac catgtttacc tgttcaatca taaaatcttc tcccgaatat
    14761 cctgaaacat tggcgaaatt atcagatggc ttgagaattc cctttacagc agattattat
    14821 aaaagacttt cccaaattga ttatggactg gctaatgaat tgctccctgt caatgctgca
    14881 attcgaagag ctattcaaca ggtagttcaa tattatactt atagacctaa aatgaaacca
    14941 ggaaatctta agaaagacgg ataacacacc ctataataaa ttggaacttt aaaccaattt
    15001 aattagattc cacattcatt aaattaccat gagaatactc ttcatattta cctgtatcag
    15061 catattttcc atatcgctat attcgcaatc aaaagatgtc atcgccagag tatatctgaa
    15121 taattcagca aagtcttact cagccgctgg ttcctcaaaa tttaccggta ccctgattga
    15181 agcacttgaa tctgaaaccg gttccgataa gtctttgcac atcgctgaat tgtttcagtt
    15241 tttccagaat aatgaattga aagccgacac ggttacttat gggaaaaatg atcccggttc
    15301 tgaattcatt tttgccaacc gtgatgaccg aagtacaaat tatgccttaa taatttacgt
    15361 ggaagaatat gatacctggg agaaaattga aggtatagaa aacgatgcaa agaaaattga
    15421 atctcttttg gccgataatt tcggatttaa tacggaactt gtttcaaatc caaccagagt
    15481 tgagctcctg atgaccatac gtaagtttac caccatgaaa tacaaaccca atgaccacct
    15541 ctttatttat tttgcaggtc atggatatta cgatgagctg tataagtgga attacctggt
    15601 tttgaaagac tcacagaaag acgaagaggt aaaacaatcc tatatgccag tttcacaatt
    15661 attgatgtta gcagacaata tatcagccga caaagtatta gtttttattg atgccggagc
    15721 aaaataattt ttatgcctga aattcatcat aattttgttg tcaatcaggg tattgaaaaa
    15781 gttttcaatg gtgtatccga cccaatttac cttgataaat ggtggagcaa aaattcctcc
    15841 ggaaacccaa gaacgggaga aatttataaa cttgggtttg gccccggtta tgcatgggag
    15901 gccgaagtaa cttctgtttc caaacctgat ttattcgagc tgaaattaat aaaggcggat
    15961 aatgactgga tgggctctaa agttactttt gagcttgtgt ctttgaatga taaaaaaacc
    16021 caggtgaaat ttcaccactc aggctggcaa tctgacagtg atcacttcag gatttcaaat
    16081 tattgctggg ctatgtatct cagaattcta aagcgatacc tggaatatga ggaggaggtt
    16141 ccttacgaaa aacgcctgga ggtgtaacta ctgagatgtt tccgattgaa tttgtccggc
    16201 aatcgcctga acttcatcca ggactcgcaa caagttgcca ctccaaagtt tagcaatctc
    16261 ttcctgggtg tatcctcttc ttactaattc caacgttaca ttaaatgttt cggctgcatt
    16321 gttccagcct tcaactccac ctccaccatc gaaatccgaa ctaatgccca catgatcaag
    16381 accaatcaag tttactatgt aatcaatatg gtctgcaaaa tcggatacat ttactctggg
    16441 tgcaacagga tcgacttccg attgtattct tggttccgcc tttgtcctca tttctctgaa
    16501 tttagcaata taggtgttcc gatccgcttc cggtagttgc ctcacttccc tgaaacttcc
    16561 taataattca aagcccattt ctgcagctac ttccttgtta ataatctgaa tgagtgaatc
    16621 ccttttcttt gtttttccct cactgacata gctggcaaaa gccactgtct ggacaacccc
    16681 tccattcttc tttatcaaca tcagttgttc atcatccaga ttgcgactgt gattgcataa
    16741 cgccctggca gctgaatgtg aagcaataat cggagcttta cttagtgcaa ccatttgctt
    16801 cattgcttct ttgccggggt gtgacacatc aatcatcatt cccagtctgt tcatctcggc
    16861 aataacttcc ttaccaagtt cacttaggcc attgtgcagc cagacactgt ctgcttctcc
    16921 cgtattactg tcacttagct ggctatgacc attgtgcgcg agagacatat agcggccacc
    16981 caagttgtag aattcttcca ctcttgaaat atttgtgcct atgggatacc cattctcaat
    17041 tccaatcatg gccactttct taccggattc aacaattcgc ctgacatcat gggaagtaag
    17101 ggcaatttcg accttatcgg gagctattgc ttccgcaaga aggtggatgg cattaaattt
    17161 atctatcgca ttttcataag cagccgcata tccctgttca tttaagtctc cctgaccagt
    17221 ataaacaata aaccaggcaa catctaatcc gccgctgtcc atttttacca ggttcacttg
    17281 agtatccaaa tccatcgtat agttgttatc cagggtaaaa ttggcagtat taatgtcatc
    17341 gtgggtatcc agagtaatta cagtctcatg gacttcccgg gctttggtta ttatttcttc
    17401 ttcagtcatt acagcatccg cgtttttctc tgcattagtg caactaaaag ccagaaaggc
    17461 agtggataaa attactaagt tcaaattttt caggtgattc atctctattt gggagttaat
    17521 tcaaaattga atataaggac aaacatgaaa atatattgaa aaggaattta gaaatgttaa
    17581 taagaaactt cttgtaatta aacttttggt tcaagcagtt agtgataatg cgcttatgat
    17641 gtgataataa aatgcctttc ttaaagttta actttaagaa agctatattt ccagagtgaa
    17701 aattcacaag tactttatta ttcatcacgc ggaaatattt atttttgcac aaaaagaaat
    17761 tctatgcgca aagttaaagt attgatcatc ttgattccgg ccttagtatt ttccggagca
    17821 ccactgctgc aagctcagga tatttacgct tcggttcaca atggatttgg agtttcattt
    17881 gttgatgcag gtaaccggct tttgacaggt caatcattaa gtaacaccaa tcgattttcc
    17941 tggtttggat ctatcgaagg tttgtattta ctgccatccg gatatttgct cggagtagaa
    18001 tttggtgctc acagacttta ctcctacaag tggtatgatg gtgttggaaa cagaactact
    18061 actatcgtta cttatcaccc cggggcaatt gttggaatag ttctgaagga taatttttac
    18121 attaaagcag gagttaattt gagagcattt tcgtatggtg gtgtttcacc gggagcaatg
    18181 attgcccttg attatgcatt gcctttgaat gatttatttt caatcccctt agggctaaga
    18241 agtgacatta ttatcgatgg taatattatc ctttctttga acgcttccat tggtttgaga
    18301 tattacataa attattgatt ttcttcattt tcagcctttt ttcagggttt atgacgaaaa
    18361 aaaaagtctc ccccaattaa ttgaggaaga cttaaatgtt gcaaaaaaag cgaatcgcaa
    18421 gggagtcgga atcaggttgt cgctacgcgg caattctcat caaaaaactc ccaacgcatt
    18481 ggtctgcaat actacttctt cttcttagca gctttctttt taggagcagc ctttttctta
    18541 gcggctttct ttttaggagc agcttttttc ttagcagctt tccttttagg agcagctttc
    18601 ttagcactac tctttttagc agcagctttc ttctttctct taggtgcagc tttctttttg
    18661 gcagcttttt tcttaggagc cgctttcttt ttagctgaac ttttcttagc agctttcctt
    18721 ttaggagctg ctttcttttt agcagctttt ttcttaggag ctgccttctt tgctgcagac
    18781 ttcttgcgag ttgctttttt cttgggagca gctttttttc ttgctgactt ccctgctttt
    18841 ttctgtttgg ccataacttg acattttaat ttgccgttaa tataacattt tttataaaac
    18901 aaattttatt tataagaaaa tattacaatt tttttttcaa gcacaaattt caaaaaccat
    18961 ttcatacacc aaaaaaattt ttgttcgtaa ggaaaattta gaaactaaaa ttgattccat
    19021 aaaaagtttt tcatgtcctt taaagaaata attttcttct gaggtaatta cgttccgctt
    19081 aaaataaaaa agaaaaaaag atcgttgcgc agaaaaatta attgcctgca gaaaagtata
    19141 catcaaaata agaaaaacta cgaaataaga atttaaaggg tgattttaat aattatcagg
    19201 tttactcaat gtcctgtcga gagaatattt tccccctccc aggaaaaata gtaaaatgaa
    19261 agcagcaagg tataagaacg acttctcttt agtagaaaaa ggatcaggag catgatgaat
    19321 aaacccggca acagccattg tgattaccag cggtactgac gcaagtcttg tccataaacc
    19381 cagaataatt aatataccgc agccagcctc cgcaaaagtt gccaaaagca aggaaacttc
    19441 ggatccaaga ccaattgggt caccaaacct ccaatcaccg gcaaatactc gtctggcctt
    19501 ggggatgcca tgtgttagca tcattcctcc aacagctact ctgaatatta gcagagcaag
    19561 atgtgattta gatgattcaa ttttggttcg tatccaatat ttcatgcgca gtacgattaa
    19621 tattttttaa ttaaggaaag cttagtattc ccaataatta caggatgaag ttactccata
    19681 ctgtaggaaa tttggccacc cggataccgg atagccaact cattcattat tctccaggcc
    19741 aatccaactc cgaaattgga acttccttta ttggtaattg ccttactctt actcatccct
    19801 ccattatccg catagtttcc ggtaatagtc acagatgttt tatcttccat ttgtttgaac
    19861 tccagcaagt attccacata tacactatat gaactcatgg gtcccaatga cttttctttt
    19921 aaactggtct gaaggctgat tagattttcc ttgtttcgaa taatatagcc ttcatcatca
    19981 agatattttt catatttctg aaagacctcc tccaatttat cggtggttat aactttaatg
    20041 gttgttgctt tctttggagc ttcctgtcca agggcttccg ttgaaattaa aattaagagc
    20101 gggataatct tccacatagc gttaattgat aatatacttt tgaatataac aaaattacct
    20161 tactccggtt tctatttatg atctgaaata ataaaatgca agtaacgcga cccggctgaa
    20221 tgcaccggcg gtgcctgggt tttatcagtt tcaaaaagcc ggttcaggtt attaccggcc
    20281 agatcttcca gcctcgattc tgcttcaact ttgtaatcgc cttctttcca tggcgagtcc
    20341 ggataaaaat taaattgttg ttcttcattc gtcaaggttg ctttgccgga tattcttaca
    20401 gtatctttcc agatgcttat cgcttcctga cacaggatat aatccagcgt ttcattgaaa
    20461 tgaataacca accgatcatt actttcagcc aggggtaata gcaacgtcca actgtcagtg
    20521 gaaggcgaaa tgctgtctct gtgggaaaca aaaaatttct tattgaattc ctctcctgtc
    20581 cccgttcctt tttttgatct ccagccaggc ataattatta aggtgtaata acgttcttcg
    20641 atcaggggtg ctccaagcct ttcattgggt ataagtcctc ttttaatccg cccggggtcc
    20701 agccacaagg tcaatattgt cttgtcttca ttccaaagct ccggaaccag gttaagaaag
    20761 attggactga cagagtctcc tttagcatct aaaactttga caaatttacc ggagtggtcc
    20821 acacccatgg gctctgaaaa ttggagatac attttcaaaa ggtttgaagg aactgtatcc
    20881 tgcccgggga aaatttccgt gatgccgggt acgctggaat tatcagattc cggaattgta
    20941 aacttagcta tcgcctcacc tcctgccata ataaggtatg ttttgcccgg agttaaggga
    21001 atcagtggtt taaaaagaat attttcattc caataaaatt caccgagaac attggtatta
    21061 ctttcattat tctccagttc aatcttcaaa tcgtctgacg catccctatc ttccaaaagc
    21121 tttgaatgaa tctttacagc ggcagctttc ccactttcat aaataacttc tacttctacc
    21181 ccttcattac tactttgaat tttacaggaa tgaaatgaaa tgaagcaaaa aaaaaccagg
    21241 ttacacaaaa agcataacct ggttatccgg attaatttct ttaccatctt ggtgaagcgc
    21301 tccagaggtc aagatttcca ttggatcttc tttgcagcat tacaaattga ttatcattta
    21361 ccttatccag ttgcagcaca ttaacccagg gcatggttgt atgttccacc ccggcaatgt
    21421 attcaacagg ttctgttagt gctccttcat agcttgccag cttatccata tcaataatca
    21481 ttaccggcct attggaatta ttcattacca aatagttctt tccatctttt ggcataataa
    21541 tcatatccaa tggtgcattt ccataaccaa attctccaac cgttcttcct tttacatgct
    21601 tgccgggctt gagttcatta agtggaaata ataccaatgg cgtgcaggtg taactcgcta
    21661 caatatactt ttctccctta atttcggttg ggataaatgt tctgacaggt gaattggttt
    21721 cataccttcc gtgatttccg tgatagatct ccagggaaga catgctttga gaatcctgaa
    21781 aaggaaatgg aatgcttctg aaagaagaac cgaattcctg attggaaatt ccggtaatca
    21841 ttacctgatc attaaaatag ttcaaatctg aaatcgacca gattcttttt gatcttcctc
    21901 tgtcatcaac ttcaccttcc cccggggcat tatgcaaacc ggttgtcgaa tatcgaatat
    21961 ccgataattt aacggctgca agcttttctc cttctaattt taaaagaacg gcggagccat
    22021 cattggtgtg aacagaaaag tataacttct ttgaaatagg attcactgcc atatcctgta
    22081 tatcaatgtt tttcacatca gtgcctaatg actctgccac taaacggtca aagttgctga
    22141 tgttaattcg ttgtggagct tctcctggac ttgtatcccc tgtttcaact gcaaatactg
    22201 cggcattcct ggtatcccca ataaatagaa ttccttccgg gccaaaggtc aatgaactca
    22261 ttgacttaat ctccggtgta cccgtttcaa atccttcaaa taagtcagcg gttttattgg
    22321 ctccggcaaa aagcacaatt aatactaaaa ccaatgttcc gtttacataa tatttcttca
    22381 tgatttcttt ttattaagat tagccaatca atttagtaaa attccatttc cctgtaaacg
    22441 gaaatctgtt aaagaaacgt tatctctata ctcaaaatag aaaaattgca ttattgatgt
    22501 aaattttagt gtttaaacag ctattcctga aattgacaaa atttattctg attgaatttc
    22561 aggattgtat gatgatggga atcaaattat tttagtaatt aagaatctgg ttatgaaggt
    22621 aaggttaata gcattctttc tcgttttatc aatttcggtt acggcacagt ttcaggcacc
    22681 aaactatgat ggaatctcaa aagtaattaa agataaagcc tctccgtact tctaccctga
    22741 attgctttcc aggtatcatg ataatgatac cgccctgagc acacagcatt accggattct
    22801 ctattacgga tatatctatc atgagaatta tgatccttat agcttgcccc tgcaattcga
    22861 ttcattaaat aatattttga aaaaggaaat cttcactcct aacgattacc aggaattaat
    22921 tgaaatggaa aaaagaattt tgatgaagtt ccccttcaat ttaagggaag tcagtctatt
    22981 ggcaaaagct tatgaagaaa caggacaaat ggaattagcc gctgccatgc atgacaaatt
    23041 tcgcaaaatt actgaagcaa tactttccac aggtgatggg aagacaaaag aaaccgcgat
    23101 gcacgtaatt atgaatagcc atgaatatga tattctgcat tatctcggac tggagtacaa
    23161 caatgagacc gctcaaacgg aaagcaccta cgaattgttg tcagtgaaac ctaataagta
    23221 taatctggaa ggaattttct tcaatattaa gaagatgttt gagcatgaag ccaaaattct
    23281 ggaaaagtaa gcttgggatt gatttctgcc tttgaaattt ttgcaattca ataaaactat
    23341 ttacttcata aattcatcat ttgattagta atatttgacg gccaagtcca aaatttttgg
    23401 tctaaactgt cacctttttg aattttaagc gtattcatcc aaaccccgca gttatgtaca
    23461 aaagcaattt aattgtcgcc tggagaaacc tgatccgcaa tttacttcac acttccatca
    23521 acattttcgg tttaacaata ggtataacag catgcttctc aatttttacc ttgattcgat
    23581 acgaatatag tttcaacaaa aatcatcctg atttggatag aatttacaga atctattctg
    23641 tttatggggg agcattcagt ggagtaaaca gaggagtatg cggagcacta ccggtatata
    23701 ttaaagaaaa ttttagcggg cttgagaacg tcgcaaattt ccacacctgg tctgccaaag
    23761 tggaaattcc cctgaatgac ggttctgtga aagatttgga cacccagtct gatttggtaa
    23821 ttgcggtacc ggattatatc gatttagttg gacaatataa ttggctcacc gggtccaaag
    23881 caattcttgg agttccaaac aaggtagttc taatagaaag tcaggcaaag aaatattttg
    23941 gtgaactgcc ttacgatgaa ttgctcggga ggcaaattat atacagcgat tcattattca
    24001 tggaaatagg aggcgtagtt tctaatacaa aggataacac agattttgct ttcactgata
    24061 tcttatcttt cgccacaatt aagagtacgt ggttaaaaga taattatgca gacaacgatt
    24121 ggggtagcac aaattccagt tcacagcttt ttgtgaaatt aagcgaaggc actctgattg
    24181 ataatttgaa tgagcagttg gaatcagcgg ctgaaaaggg cgatgcggaa accgattcag
    24241 aatggacgac cactttttca gctcaaccct tatccgatat tcattacaac acagagattg
    24301 gtgtttttga taatgtttct cacgttgcca atctggatac tctccgcaaa ttaacctgga
    24361 tagccattgc tttgctggtg attgcggcta ttaatttcat aaatcttgaa acagcgctgg
    24421 caacaaagcg ttcccacgaa gtaggaataa gaaaagtgtt gggcagttcc agggttcggc
    24481 ttgtgtttca tttccttaat gaaagcttta tgattacaac cgctgcggtt gctctatcga
    24541 tacctctgat aatggcttcg atttccattt ttaaagatta cattccggag ggggtttcct
    24601 ttaatctttc agataccaca acattaatct acctgggatt aaccattgcc atcgtaggat
    24661 tgttggcagg tctctatcct gcttttgtga tgtcttcctt ctctccggtc aaatctttaa
    24721 aaaatcaaag cggaagaaaa aattccctaa gtgcaaattt gagaaaggga ttaacaatat
    24781 ttcaatttgg tttttctcag atattaatta tagggacggt tatagttgga cttcaaattc
    24841 attatatgca ctccaaagac cttgggtttg aaacgaaaag tattctttat ttttacactt
    24901 cttactatgc agagttaagc aagaaagatg ttcttaagaa tgaattgcag agaattccgg
    24961 aaatatcaag cttaagcaat ttcggaaatc ctccggctca acgcggatat agcacaagta
    25021 ttaccaaatt cattaatgat aaaggcgatg aaattatgtc taacgcgcac caaaaatcgg
    25081 gtgacacaac atacatccct tttttcggat taaaattcgc cgcgggaaga aattaccggc
    25141 cgaatgcggc ggggaaagag acggtgatta atgaaacttt tgccagggag ttgggatttg
    25201 aagattcttt tgatgcactt ggaaatatat tcaggcgtgg taatgatacg cttacgatag
    25261 ttggagtgct ggaagatttt cacttccggt ccctgcatca taaaattgaa cccataatga
    25321 ttacactgga caatacggca aggtcatttg gaattaagat gacggaaagt gccaatacgg
    25381 accaggtgat tgaaaaaatt aagaatgagt atgcaaaagt ttatcctgat agaacttttg
    25441 cccattactt cctggatgaa acagtggaac aattttacaa gacggaacaa cgggcatcaa
    25501 aattagccat tacggcaacg gctatcgcga taattatttc ttgcctgggc ttgtttggcc
    25561 ttgcttcatt tactgccgtc caacggacca aggagatcgg catcagaaaa gttttgggtg
    25621 ccaccgcgga tatgattgtt tccctcctgt ccaaagactt tctcaaattg gttctaatct
    25681 caatagtaat ttctgttcca attgcttatt ggattggaga actatggctg gaagattttg
    25741 cctacaaaat cccaataagc ggctggattt tcgtgctcag cgggcttggc tctatcctga
    25801 tagcattttt aacagtcagc tatcaaagca tcaaggcagc catggcagac ccggccgaaa
    25861 gtttacgcta cgagtaggtt taaatcaggg aatatattat taccaggtgc atcaggaatg
    25921 agatcaggat gaaatgcttt aaactccggt atttctttct gaggatgaga tatccgaaaa
    25981 tataatagac aaccattatg ggtaaaaaca gtaagatggg attaccctga acgaccaatg
    26041 cagccaggcc ggcgactagc aataaaatcc aaatgaaata atatttgatg aacgtaagta
    26101 aagcatttaa tccttttgaa gtctttctga gaaggagcaa aaaggtagcg gagaaaataa
    26161 ttaaataagc aaaagttcct gttttcaaca aatgaaataa aggaacgctc aataagggac
    26221 caatacttaa cgatttttca tccatacctt ccaaataggc ttcatcaaat tccgaatttc
    26281 gcaatccctt tttagcttct tcagtaacat gagacaactc caaaacctgg ttcaaacttt
    26341 gaaaaggtcc aatctcatcc ctgtaccgga tcaattccgc agcactttgc ccgtccattc
    26401 ccggaactga gagtaaatca actccctgtg ccgcattaag attaaaagta tacacgggaa
    26461 gcgtaattgc acccaatgca tcaagtgcaa gtacgcgatg attatagttt ttaaccatca
    26521 gccaaatttc gacaggataa tccggtgaat aggcttcacc gctcagttcc tgatatgttg
    26581 aaagaatgat ttctgattca tcaggaaatt cttccatata gccttcaatg aaatccatta
    26641 actgagctcg atcagactgc tctaaccgta catgcttatt caacacatgg aaatacttca
    26701 caaataaatt ttccacttca ttaaactttt ggaccggatc tatcacaccg atggtatcta
    26761 ttagaaagtt ggaataaaat tttgcattac ggtagtattt gcccagacca tttaccgcga
    26821 gtcgtgaaaa aaatgctgac acaaaccctt ctgagcacat caattgtgcc ttattgcgtt
    26881 tattgtccag gtggataaca ccggcatttc gcaccgacat catatcctga acgcttgaaa
    26941 gggccggata tccattctga aacgtcactg ttccttccaa tgagtgctta tagcgcttga
    27001 aatcctcata ttgctggtac cacactaaca ttgtgcattt ataataatcg aggcgtagag
    27061 gccagttaaa atcccttaca aacgattctg aagcgcgcaa taagtttggt tctctgaccg
    27121 ccacatcatt ttcgattcct ttcctgatgg tttcatttct ctcatagatc cgcgaaatat
    27181 tttccatatg ctctgcaaac ccttcgttaa acgctactga attgtctgtc atcaaactaa
    27241 aataatgaac agttacactt ttggcttgct gctcaataga atccgtggaa ctcaaaagtc
    27301 gaaaaattac atgtcctaat tcatgaggat ataactgtga aacggacata actctatcca
    27361 attttgatgt gatgctgtta gccgtcagat ccacgtaata ggcgttactc ttgtttatag
    27421 taccttccaa agtcttaatc tgaaaacctt ttttagcaaa gccgccctgg tttttggtga
    27481 tagccaggta cgcaggctcc ctcgcagaaa cctggcctgt atttattaaa taattttgag
    27541 caaaatgata aagttgaata gcttgtccta taaaactttc ttccaaagcc ttttgggata
    27601 ccttgtaaat attggctgta tcggggacca attccattaa aggcatctcc cctttcccat
    27661 cgtaaaccgg ttggataaga acaaaactct tttcagattg ggccaacaaa ctcgcggaaa
    27721 caaaaacaag caacgaagca gaaatcactt ttatcataac tattcctttt aagaatgaga
    27781 caagcaaagg atattgctag ttacagcatc caccggaaaa atcagtaata tattatttac
    27841 ttgattatct cagaaaaaaa ccttcttgaa acgggtcctc cgggtcgagt agaaactcat
    27901 gttgcccggt aataaaagca gtgccctcaa ccctggggat aactgcatca aattcaccaa
    27961 acttaacaag ttcaacaact gatgcggtga agatactgcc ggttatgctt tcgattttca
    28021 tctcttcgcc aatatgtaaa tctccttttt tgtaatgaag tgccatccgg ccgcttactc
    28081 cggatcctgt gggggatcta tccacctccc cgttagcaaa aatgcataca tttttactgt
    28141 cggctgtcaa agatttattg ctgatgaaaa ttgtgccata aagaaaactt aaatcaggtt
    28201 caatggggtg tttaatatgc ttatttgttc ttattacttc ctttttgatt tccattccta
    28261 aatggatcaa gccggtataa ttttcctggg ataaatcaat ttcaaattta tcggcgtcta
    28321 cgtaagcata aaaagctccg ccataagcaa ggtcataagt tatagcacct atagacgcgg
    28381 tgttcaatgg tatatttact ccgaccacaa aagatggaac tccatgaaaa taaatagacc
    28441 tggtttttcc attttgaatt cttgcaaaac tttcgatggt accacacgga gcatcaattt
    28501 tcatccttgt aacaggttct tccatgttaa tccatgcttg ttctatggcc aatttagtaa
    28561 tcgcgattat tgcatggccg cacatcgtac tatacccctc gttatgaagg aaaagaatgc
    28621 caaaatctga tgaatcatta ttgggaggaa gtaaaacgca gccatacata tcggcatggc
    28681 cgcgaggttc gagcataagt aatttgcggt acttatcaag attttctttc atgtacctgc
    28741 gatattgaag cactgatttt ccttccagtg ttggcaaacc ggatagaatt acgcgcagtg
    28801 gttcaccacc tgtgtgcata tccaccgttc gtatacttat ccagtcgttt ttattttgaa
    28861 aaaattgttg tttttgaatt cgctggaagg tgctactcat cgcgaacttt ttgat
//
LOCUS       DASWHX010000204        21399 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_1325161, whole genome shotgun sequence.
ACCESSION   DASWHX010000204 DASWHX010000000
VERSION     DASWHX010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 21399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21399
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1325161"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..289
                     /gene="trxA"
                     /locus_tag="VGA21_13495"
     CDS             2..289
                     /gene="trxA"
                     /locus_tag="VGA21_13495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017731547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEX9651572.1"
                     /translation="MVTKSEKPVLVDFWAEWCGPCKMIHPVVEELAADYEGMAVIGKV
                     DVDNNPNVSFKYGVRSIPTLLVFKNGQVVDKQVGAVPKGVLSQKLDAQLAS"
     gene            complement(372..3218)
                     /locus_tag="VGA21_13500"
     CDS             complement(372..3218)
                     /locus_tag="VGA21_13500"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEX9651573.1"
                     /translation="MKTFLEHIADDIELKSPDEMRDLCFVFPTKRASLYFRSLLADKF
                     SGKVFWGPSILSIEEFINACSTQVPSDEITLVLELFTVYKKHDPEVVLDKFFNWGQIL
                     LKDFDEADRYMVNTDKLYRNIREYQELEVLFGDNEETKNAMEAFNKVVQVQDKTQLMA
                     MFIKTWGIISKVQHNFQEHLAKKGLAYQGFLYRKLAEDLKAGRVTPYKQVILCGFNAL
                     SASEEVIFDALLINGKGKVYWDTSEFYINDADDAGKFLKRYKKKWKQKESIWINTDVS
                     LEEKQIHIIGTPELVGQGKLAANELEKITSDKINEVRTAIVLGDEKLLFPVLYAFPEN
                     IESINVTMGYPLNSTSWYSLAESFVSLWKENQDSKNDYVFNRKELLIFLGNPLIKALY
                     EGKKDRLLEIQEIKRSRLRMSEIQPFLKNKLIETALRSEKSGMSVLQNLQDLLIKISL
                     ILTRGEKSMEEEFTYHLVKNLVRLRETLDSNSLNPQPELLLKLIREIMISVRIPFSGE
                     PLKGVQLMGFLETRALDFDNLFMLSLNEGIIPSGKTKTTYIPYAIRKAFKMPTFEELD
                     AIYSYHFKRLLQRGKKIFLFYNTEVAVDGSGEKSRFLRQIKKELKPFENITIHERIIS
                     PVISSRKDYPIIKIPKTREILTDMNQFVVNGNEKPRRLSPTTLTTYIECSLRFYFKHV
                     ARMPELEEFNEDMDPREFGNIVHNALEKIYKPWEGREINSGEIKGILSGSIISEKVAE
                     AYAENKYSVEKELEGKNLLNRDIIEKVIEKALEQDALEAPIKIISLETDSLNKELELG
                     GRKVYIGGNLDRLDYLPLKNTFRIIDYKTGKVDLLPRTKRQHHNFDEYLEDYFTNSKY
                     KSGFQAYYYAYLFYKSRPGENVTAGIFGLKSVNQGIQYLRNGTTIDPKLLDVYESRLE
                     NLISEIFNEEIPFVETDDHKKCEYCSYRSICRKN"
     gene            complement(3215..6454)
                     /locus_tag="VGA21_13505"
     CDS             complement(3215..6454)
                     /locus_tag="VGA21_13505"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEX9651574.1"
                     /translation="MKKTPSQLDLFSMRMKAHPEGGKLTIFQSSAGSGKTFTLVKEYL
                     KIILKDPSNFSHVLAVTFTNAATKEMKDRILDVLEELAAGNKSEMRKAIEDDFKSELQ
                     DHLPIEKRAAEALTKILYNYSHFDVSTIDHFFTRVVRALAYELKQPVKYDIDVDNERA
                     VENAIERLFGEIHTNKETRNWLKEFALSRLDEDKGWYIEYSLLELGMELFQEKFRENF
                     DDAGKKIDIQQLKKLADGLVFIKESYTRDLKALSKRAVDLISDFDLEIIDLKGGSRSV
                     ANGFIKILDGDFKLTDTFIETCNGYQDWFSQSSRKRARIKQLVDGGLDEIGKQLAEHY
                     SKFNTAFITANELLKNIYSYGLMGEMNKNLQKYKLENNLLLLSDIGSMLRQVIGENDA
                     PFIYEKLGSRYKHLLIDEFQDTSNFQWINLKPLVINSLSNGYKVYLAGDVKQSIYRWR
                     GGNLHLILSDVKKQLDQYGKIDETPLNINYRSAKNIVEFNNSFFEIARKILQLHPEVN
                     PDDYLITQAYQHVKQKSFRDDEGYVQVNFLEKPDEYEKHWHEAAKVKVVDTISECLQD
                     GFQYKDILILVDVGRLANELADHLSGANIPVITENSLLVENNQKVKMILSILNWLDNP
                     GDIVSKAAILHHHLEICEGRERIFDHDIYSRENQHEFFNKTIPKELIETAASLKQLPL
                     YELVEELVIIFKLNDPPDAFVQKFQDICLRLASRGIISIESFFNWWNKKITRKDKFND
                     LGVKVPSETDAVEITTIHQAKGLQRNIVIVPFAGDRFEMRTWKDTIFWTSELPPKFQE
                     YNLLPLRFSDNLIDSEFASAYKKELMEGIVDRLNVAYVAFTRAEDRLYIFTNINKKPE
                     NPYGNLHRLIYGVFTDREFPFQSDFDLENNQFILGSPSKKKIKPDESLIEHITKFPSR
                     HYSEKVTIRKDSQKFFLLFDNTTAKKIREGIVVHAAMELVSSEGDVVRTLDRLEAQGL
                     LRRNEKKNIAGKVSEILLKSEIGKWMETGWIPIKEHEIFSRGEIFKPDLVLVKDNKAI
                     LIDYKREKRSAAHEKQILNYADILTAMGLQIEKCLLIYLDEFEIVEVKK"
     gene            6622..8940
                     /gene="ligD"
                     /locus_tag="VGA21_13510"
     CDS             6622..8940
                     /gene="ligD"
                     /locus_tag="VGA21_13510"
                     /inference="COORDINATES: protein motif:HMM:TIGR02779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HEX9651575.1"
                     /translation="MAKSSTRVEIGKRKLELSNLKKILYPKDGIVKAQVIEYYLKIAP
                     TILSHIKGRPLTLVRFPDGIEGERFYQKNRPDWAPDWIEYETLGKEAKDYMIATEAAS
                     LVWLANLACLELHQMHSRRPNFDKPDYMVFDIDPPEGYEFTKVVDLALRLRQHVESYG
                     YATFPKTTGGKGIHILCPVEVKYSFDEVFQTAKGIAEPFVKKNEKETTLQIRKEARKG
                     RVLIDVFRIRQGQSIISPYSMRGVPGAPVSMPLSWEEIEATKKSTEWNINTAMERVLQ
                     EGDAWEGINAYATSLHTKLKGKKIVKNLGPNSKYKTPETLESYKKKRDFRKTPEPDAN
                     YAGGDGNGFVIHRHHASHLHYDLRLEQDGVLKSWAVPRGMPPRPGIKRLAVATEDHPM
                     KYLNWEGTIPKGQYGGGDMWIYSLGKYEIVKEKKNGFYFNLYSKDLNGEFRMHLMKKK
                     EWLLERVNNPQINYLQDPIEFMLSTSEEEPPKGDYFYEVKWDGIRVMISLDEGEIRIR
                     SRSQRDITAQFPELLIPEEAFRATCGLFDAEIVCLDEKGRPDFKKVIHRLMQSTESGI
                     KKGAARNPVYCYFFDCLYLDGRPIVNEPLWRRKDWLKDAIKPGTAYRVSEIVEEGEEL
                     FEAAKKMELEGIMSKDPESRYLPGKRSNSWIKIKVRNTVDCVIVGYTEGKGDRKKYFG
                     ALQIIQINNGDPVYRGKVGSGFDSKLMKSIMEELEKLKKIKNPKIENPVDPAVTTWVE
                     PKLFCEVQYAMITNNETFREPVFIRMRPDLEE"
     gene            complement(8981..9289)
                     /locus_tag="VGA21_13515"
     CDS             complement(8981..9289)
                     /locus_tag="VGA21_13515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651576.1"
                     /translation="MKLPEDIVKKINELFPGNEENLIVWNLINEILQKKINVGHAQLI
                     RSVLILSEGDKEVIRKLIQSNFNGDPRDVIMEADCKEGKPGHYFIPLFKQEMILQAKI
                     "
     gene            complement(9954..10259)
                     /locus_tag="VGA21_13520"
     CDS             complement(9954..10259)
                     /locus_tag="VGA21_13520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651577.1"
                     /translation="MLEKIFIIIATLLFFPVASVTLGIMLDSIFEARYFKEAGAIGYA
                     FYFLLFGALIGIFCCGLILFHKKIPFFRDRPIVLIHFAFLFIMFISYYFYIRSDVRY"
     gene            complement(10279..12297)
                     /locus_tag="VGA21_13525"
     CDS             complement(10279..12297)
                     /locus_tag="VGA21_13525"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing sensor
                     histidine kinase"
                     /protein_id="HEX9651578.1"
                     /translation="MIKPFLKITFLLVLAWPAFSQPENINDLKTVRDSLSSDTGRILV
                     SIELIEALWRNDNDEEAMKEAETAYSLSQNIDYDIGTGMALMHIGIINYRKSYFRGSL
                     TSLLEAIPYFEKVGRKSDLALVFHWTGNSYYKLSQFEKSLEYYLKSLRLREEIGDEQG
                     TAFSYYNIANIYFDQKQYETALDYHFKSLAIKGKLNDSRGIAFSFNNIANNYMELQEV
                     DKALDYYQQAYVLFREINHQQGIAYTLGNIARIYKKNKQFSKALESLLQAKAIMEEIN
                     DKQALIEAHNQIASVMISLRQYSSSEKHLLYGQLLARETGSEEALIDNYLEFSRLDSA
                     RNNMKGAFEWHKKYVAKKESIFDTQKSKQIAEMQAAFDLERKDQEIVLLNKETELHRS
                     QRKNQLVIFACVLLTMFAVMSGLMYFLRQKQKTNRKLEEQKKYSEELNRLNDKLFSII
                     SHDLRNPLISLKSILELAANQRITKEELTVLLSSIGDNTQHVMDLLDNLLNWAREHLS
                     GDPVAFENMNLNEIVQNAINLLKPMADQKNIRLVDQLSNSAIIYGNRNMIELVVRNLL
                     SNAIKFTQNMGEVIINCELNASYVTISIRDTGIGIPHDQLSKLFGTKNFSTRGTANEK
                     GSGLGLILCREFVEKNGGTIWVRSTEKKGSSFYFTLPMAQSLPAVITA"
     gene            complement(12332..12580)
                     /locus_tag="VGA21_13530"
     CDS             complement(12332..12580)
                     /locus_tag="VGA21_13530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651579.1"
                     /translation="MRFNYNDLTRVVEPHCYCNTVKGDEALRACQVGGNSSTGLPGWK
                     LFHLSKIGSIQILDENFNDARPDYKPGDKGMQVIYIEI"
     gene            complement(12561..13190)
                     /locus_tag="VGA21_13535"
     CDS             complement(12561..13190)
                     /locus_tag="VGA21_13535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651580.1"
                     /translation="MEKCLNKKPHQRFNRLIKTEYETKYRGDYINKWKTIVSAKDLKK
                     ALLKHFSYLADSPGYTKVLKSIIQKVKQARIVKNALEIKFMGGDVMSCHAPSTKSSLN
                     SYPVSFQKIVSQHEITVVKEKMLLGDDRGVEAYFDETLIGFNKLGRVKTLAAISRLHS
                     KCYMITRNWEDITSPGCAKKLGLGIVSSFHETPDHQCNLKQKIGAIQLQ"
     gene            complement(13325..14491)
                     /locus_tag="VGA21_13540"
     CDS             complement(13325..14491)
                     /locus_tag="VGA21_13540"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HEX9651581.1"
                     /translation="MKKNLVYKDSKSDKFWQIEVAGNSFTVTYGKTGTAGTSQTKSFD
                     SEEKCFKEAEKLLNEKLRKGYKESKGATKVVAPSPAPKKKAGLTMKPADFTRIENYIK
                     KSVSDFEGEVKDELDTDGDMGEFADSLWENFIMSGAEHLVDELKLGQKESNKYYDFFR
                     KKFHEEFERLVSSFPIAVPQSKQEIDKELEEAVVKRNWHKVTKLINEGADPNTSSVLS
                     RAVASSQTTLVDLLLTKGADPNIADNGGFTSFDLAVGSLPLSTIKLFIKFGAQKTPKL
                     FFISVQNDSIIFFHLLEAFHISEMEKTNCFTEAGYRGNREIIDFFLENDMDINSKNEW
                     GYTILHAAAMRPNDKNIAVIKFLLEKGADKKLKDKDGKQPFELTEGKHKKLLLP"
     gene            complement(14614..15372)
                     /locus_tag="VGA21_13545"
     CDS             complement(14614..15372)
                     /locus_tag="VGA21_13545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651582.1"
                     /translation="MVTHPGWWLLERMLERTTAVLGSGLEAPVSREQIEQAWLPAGLK
                     TILADHLGAAAAIEEQSSYAILRAVVGVPPIVRHARTDTGYIRLVAIARNEQLVDSDP
                     LVFARLGENDTVANEPECFITELEEGESWRANRLGYTFDRVLPWRWAWPFTSIVYPVG
                     DEVMSLDWNYSLDEMATVLDRGLGLKAGATFEAGWVVGRIEERGAWLAVRRIGNNFRL
                     CLGGERDAVRRLQELKQTLTELQIEICGENEEEE"
     gene            complement(15455..15817)
                     /locus_tag="VGA21_13550"
     CDS             complement(15455..15817)
                     /locus_tag="VGA21_13550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020607345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651583.1"
                     /translation="MTNIKTRIDEWSVKDLEDNSSLNIIVESCTELGNNSKPGIQIIY
                     MGNIVNFEPNVVEQWAYKAGKEGVTDYLLSDKSWMVHTDRYIKIYLVTGSPLKAKVEV
                     KTRSSKLVVKEYELPFEV"
     gene            complement(15917..16081)
                     /locus_tag="VGA21_13555"
     CDS             complement(15917..16081)
                     /locus_tag="VGA21_13555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651584.1"
                     /translation="MKINRREFVKYSTVSIIGVAVNLNAHLGVGSIESSLTISAALAG
                     RSGFSDGKSY"
     gene            complement(16129..17457)
                     /locus_tag="VGA21_13560"
     CDS             complement(16129..17457)
                     /locus_tag="VGA21_13560"
                     /inference="COORDINATES: protein motif:HMM:NF016274.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HEX9651585.1"
                     /translation="MKNLKKPLIYCLLSAISLYACVPLEESKAVSAAEVSDTSFYTFP
                     AEFEEHEAVWIKWTTETYLGNSTDGVIKAVIKALNPFVKIKLVVSDKEEESEVKRILK
                     EEGIPYSHVEFFINPNCTDRWLRDNGPVFVRNNEGQYKIVDFGFNSWGVGKQEDPINI
                     DIDVTDRSFARILNIPAIRSSMISEGGDRDFNGMGTVMVTESVELQRNPDMTKEQIAE
                     ELKRVLGQKKVLWLRTGPIEGDQMKMKGALPGNAFTSTTCGHIDEYARFADPNTIILA
                     QVSEEERQSNEIMSMTYDILEENYQMLKKETDQDGNPFNIIRIPVTDHYYVSLAYSKE
                     IPWYELSWYDDLRYFDGTPVLEGDTINQLIASSYLNFLVTNGCVLVAAYYKEGRPLST
                     RQKDEQAKEILQQAFPGREIIQIHKAEDLNSNGGGIHCMTQQQPKMLTGN"
     gene            complement(17488..18846)
                     /locus_tag="VGA21_13565"
     CDS             complement(17488..18846)
                     /locus_tag="VGA21_13565"
                     /inference="COORDINATES: protein motif:HMM:NF025680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HEX9651586.1"
                     /translation="MKSIKNRYIFKLSILVFLILFNGCNVLDQEPKNQFDGQIPITDK
                     FSAQGALNGLYDALQEIDYYGAYFPISVDMFTEDLVISAFWDSYKHLDSHYVPATSEN
                     IKLIWLQMYRTINVANNIIYYVPGVDDPLFTQDEKNEIVGAAETIRGMVYFDLLRTFG
                     EHWDSGSSFGVPIRIEPAFNAEFTPRSTVAQTYQQIITDLQNAESKLPDAYHPAYMNK
                     QTVAALLARTYLYLQDYEKASEYASAVITSGNYALQENYASVFSQKNSSESIFELNFS
                     AQDPSYYLREFGERDEATISPDFFDIFDENDTRRATIFPDFDNENLLTTEKYITQDAS
                     AFIIRLAEMYTIRAEANAILLNDPNAGIADLNVIRERAGLSALQPGDLQNMPDFINIL
                     LEEKRREFYAEGHRYFDLARHQKMEEVYGIENFRSIFPIPFVEILTASGLGLTLQQNP
                     GY"
     gene            complement(18898..>21399)
                     /locus_tag="VGA21_13570"
     CDS             complement(18898..>21399)
                     /locus_tag="VGA21_13570"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HEX9651587.1"
                     /translation="EDFPDYLNTKSNSITDINPADIASIEILKDASATAIYGSRGANG
                     VVLITTKRGKEGKGIMSLNHYSGVQLGPTNMIEMMDSREFLELMNEASANDGLGENFY
                     TNQMGLDINDPELQNTNWYDEIMNPAPICQTQFSAQGGSNNIRYYTGLTYFDQEGVQI
                     GAGFKRFNGRLNLDFDASDRLTIGTSLGISRSILKRTETDASFYGVVVNAQAGDPTMP
                     VYEADGSYADPFNYGSWWMLENPVKAANEYNRYTNTSRLLGTIFGEVKLTSWLKFKTA
                     WSVDYTDLKDEQYISSLTWESIRAGANGLGQYSTYQDLTWLSENTLTFDYSAGSSHRF
                     NAVLGYSAQESQRDDSDIRARQFPMDGEGKLGSASSVTVGYSGGYSWGIESYFARLNY
                     AFKGKYLLTLTTRADGSSRFGINNKFGIFPSGSVAWRISEEGFMKESNLFSDLKLRFS
                     HGLTGNQEGISNFASRYLWALNASYNGQGGMSPSKKGVSQIANPDLTWEKTTQTNLGI
                     DIEVFHGKIGLIMDAFVSNTKDLLLSVPVPGTSGTSVMTKNAGKLRNKGLEIALNSVN
                     LDRALTWSSGFNISFIRNEVVEVLNDGEPQDQSHILMEGMPLGTFYMIHFLGVDPQTG
                     DAMFEDIDGNGVINSDDSQVVGTAQPDFFGGLSNTLSYKNFNLDFFFQFNVGNKIFNR
                     SKTQIEQLGYNYLYFSDEDYANGDVYILPNGNNSISVMDRWRQPGDITDIPRASVKTK
                     NYQDFSDQWLEDGSYLRLKNIELSYTLPNSLLQRTNVFKSIRIFVQAQNLLTFTKYTG
                     FDPEIETSSNLLTQGQDYSGLGQMRTILAGFNIGF"
BASE COUNT         5995 a         4588 c         4095 g         6721 t
ORIGIN      
        1 aattgtgacg aagtctgaga agcccgttct tgttgatttc tgggcagagt ggtgtggtcc
       61 ttgcaaaatg attcatcccg ttgttgagga gcttgccgcg gattacgaag gaatggcggt
      121 tatcggcaag gttgacgttg ataacaatcc caatgtgtct ttcaaatatg gtgttcgatc
      181 catacctaca ctgctggttt ttaagaatgg gcaggtggtg gataagcagg ttggcgccgt
      241 tcccaaaggc gtattatctc aaaagcttga tgcacagctg gcatcgtagt tatacctaac
      301 attttccttc aagccatgtc atttgatatc gcctgtgact ttttcagcag aaacgggaat
      361 gactacaaaa actaattctt cctgcaaata cttctatacg aacagtactc acatttcttg
      421 tgatcatcgg tttctacaaa gggaatttct tcattgaaga tctctgaaat tagattttca
      481 agtcgtgatt cataaacatc cagtaacttt ggatcgatgg tggttccatt gcgcaagtat
      541 tggattccct gattcacaga ttttaatccg aaaatgccgg ccgtaacatt ttctccgggg
      601 cgcgatttgt aaaacaggta tgcgtaataa tatgcctgaa agcctgattt atatttcgag
      661 ttggtaaaat aatcctccag gtactcatca aagttatgat gttgcctttt cgttcggggt
      721 aataaatcta ctttgccggt tttgtaatct attatcctaa aagtgttttt taaagggaga
      781 taatccagcc ggtcaaggtt accaccaatg taaacttttc tgccacccag ctccaattct
      841 ttattcaatg aatctgtttc caaagaaata atcttaattg gtgcctccaa tgcatcctgt
      901 tccagcgcct tttcgatcac tttttcaata atatcccgat tcagtaaatt tttcccttcc
      961 agctcctttt ccacgctgta cttattttcc gcgtatgctt ccgctacttt ttctgaaatg
     1021 atactcccag acagaatccc ctttatttca ccggaattga tctccctgcc ttcccatggc
     1081 ttataaattt tttccagtgc attatgtact atgtttccaa attcccgagg atccatatct
     1141 tcattaaact cttccagctc cggcatcctc gcaacatgtt taaaataaaa tcggagcgaa
     1201 cattctatgt aagtagttaa cgtggtagga ctcagccgcc tgggtttctc atttccattc
     1261 accacaaact ggttcatgtc ggtcagaatt tccctggtct tgggaatttt gataatggga
     1321 taatccttac gagaagatat taccggcgaa attattcgct cgtggattgt aatattttca
     1381 aatggcttca gctctttttt tatctgtctc aaaaatcgac ttttctctcc tgaaccatct
     1441 actgccactt cagtattata aaaaagaaaa atctttttac cccgctgcaa cagccgcttg
     1501 aaatgataag aataaatagc atcaagttct tcaaaggttg gcattttaaa agccttccgg
     1561 attgcataag gtatataagt tgtttttgtt tttccggatg gaataattcc ttcattcagg
     1621 gaaagcataa ataagttgtc aaaatcaaga gcgcgagtct ccagaaaccc cataagttgc
     1681 acgcctttca atggctcgcc tgaaaaagga attctcaccg aaatcatgat ttcacgaata
     1741 agcttgagta ataattcagg ttgcggattg agcgagttgg agtctaatgt ctcccttaac
     1801 ctgaccagat ttttcaccaa atgataagtg aattcctctt ccattgattt ttcgcctctt
     1861 gtcaaaatca atgagatctt gatcaggaga tcctgcaaat tctgcaaaac cgacataccg
     1921 gatttctcag atctcagagc tgtttctatc aatttatttt tcaggaaggg ttggatttca
     1981 gacattcgca atctgcttcg ctttatttcc tgaatttcca ataaacgatc tttctttcct
     2041 tcatacaagg ctttaatcaa cggattgcca aggaatatca ataactcttt tctattgaaa
     2101 acgtaatcat tctttgaatc ctgattttct ttccaaagtg aaacaaagct ttcggctaat
     2161 gaataccatg aggttgaatt caagggataa cccatggtta cgttgattga ttctatgttc
     2221 tccggaaatg cataaagcac cgggaaaaga agtttttcat cccccaaaac aatggccgtc
     2281 ctcacttcat tgattttatc cgaagttatc ttttctaact cgtttgcggc aagcttccct
     2341 tgtcccacaa gctcaggcgt accaataata tggatttgct tttcttcaag agacacgtcc
     2401 gtatttatcc agattgattc cttttgtttc cacttctttt tatagcgttt aagaaatttt
     2461 ccggcatcgt cagcatcatt tatataaaat tcagaagtat cccaatacac tttgccctta
     2521 ccattaatta gtagtgcatc aaagatcacc tcttcactgg cggacaaagc attgaagccg
     2581 caaagaatga cttgcttgta aggtgtaact ctaccggcct ttaaatcctc tgcaagtttc
     2641 cggtagagga atccctggta tgctaaacct tttttagcca ggtgttcctg aaaattatgc
     2701 tgcactttac tgataatccc ccatgttttg ataaacatgg ccatcaattg agttttgtct
     2761 tgcacctgga ctaccttatt aaaggcttcc atcgcgtttt tcgtctcttc attgtcacca
     2821 aacaaaactt cgagttcctg gtattcccgg atattgcggt acaatttgtc cgtattaacc
     2881 atataacggt cagcttcatc gaagtccttc agcaatatct gaccccagtt aaaaaattta
     2941 tccagcacaa cttcaggatc atgcttttta tagaccgtga acaattccag caccagcgta
     3001 atttcatccg aagggacttg agttgaacaa gcattgataa actcttcaat gcttaagatc
     3061 gatggtcccc agaaaacttt tccggaaaac ttatcagcta acagacttcg aaaataaaga
     3121 cttgcccttt tggttggaaa cacaaaacac aaatcacgca tttcatcagg cgacttcaac
     3181 tcaatatcat ccgcgatatg ttccagaaat gttttcattt tttcacctcc acaatttcaa
     3241 attcatcaag gtaaatcaac aggcattttt ctatttgcag tcccattgct gtcaggatat
     3301 cagcataatt caggatttgt ttttcatgtg ctgcacttcg tttttcacgc ttgtagtcta
     3361 ttaaaatagc tttgttgtct ttcactaaaa caagatccgg tttaaagatt tcacccctgc
     3421 taaatatctc atgttctttg atgggtatcc atcctgtttc catccattta cctatttcgc
     3481 tttttaaaag aatttcagaa acttttccag caatgttttt cttttcattt ctgcggagta
     3541 atccttgtgc ttccaaccgg tccaaagttc ttacaacatc accttcagat gaaactaatt
     3601 ccattgcggc gtggacaaca ataccctccc tgattttctt tgcggttgtg ttatcaaaaa
     3661 gcagaaagaa tttttgagaa tcttttctga tggtaacctt ttccgaataa tgccgggaag
     3721 gaaatttagt aatatgttca attaaggact catccggttt gattttcttt tttgaaggtg
     3781 atcctaagat gaattgatta ttttccaagt caaaatccga ctggaaagga aattcccgat
     3841 cagtgaaaac tccataaata agcctgtgca gattcccata aggattttca ggtttcttat
     3901 ttatattggt gaagatatat aagcgatctt ctgccctggt gaatgctaca taggctacat
     3961 tgagacggtc cacgattcct tccattagtt cctttttgta cgcagatgca aattcagaat
     4021 cgatcaggtt atcactgaac cgaagtggca acaaattata ttcctgaaac tttggcggca
     4081 attcggaagt ccagaaaatg gtatctttcc aggtgcgcat ttcaaaccga tctccggcaa
     4141 agggtacaat tactatatta cgctgcaggc ccttagcctg gtgtatggtt gtaatttcta
     4201 cggcatctgt ttcggaggga actttaactc ccaaatcgtt aaatttatcc ttgcgcgtaa
     4261 tctttttatt ccaccagttg aaaaaagatt cgatgctgat aataccacgg ctggccagtc
     4321 ttaagcagat atcctgaaac ttttgtacaa aagcatcagg tggatcattc aatttaaaaa
     4381 taatcactaa ctcctccacc aattcatata aaggaagttg ttttaaactg gctgcggttt
     4441 caattaattc ctttggtatt gttttgttga aaaattcatg ctggttttct cttgaataaa
     4501 tgtcatgatc aaaaattctt tctctgcctt cacaaatttc gagatgatgg tgaaggattg
     4561 ccgctttgga aacaatatcc ccgggattgt ccagccaatt caaaatgctg agaatcattt
     4621 tcactttctg attattttcc accaacagtg aattttcggt aattaccgga atattggcgc
     4681 ccgagagatg atcagcgagt tcattcgcca acctgccgac atcaacaaga atcaaaatat
     4741 ccttatactg aaatccatcc tgcaagcatt ctgaaatggt atccactact ttcactttgg
     4801 cggcctcatg ccaatgcttt tcatactcgt ccggtttttc gaggaaattt acctgcacat
     4861 aaccttcatc atctctgaag cttttttgtt tcacatgctg ataagcctgt gtaatcaaat
     4921 agtcatcggg attcacctca ggatgtagct gcaaaatttt ccgggctatt tcaaaaaatg
     4981 aattgttaaa ttcaacgata ttttttgcgc tccggtaatt tatgttgagc ggtgtttcat
     5041 caatcttacc atattgatcg agttgttttt taacatcaga caaaatcaaa tgcaaattcc
     5101 cgccacgcca gcgataaatg gattgtttaa catcccctgc caggtaaacc ttgtaaccat
     5161 tgcttagaga attaattaca agaggtttta aattgatcca ctgaaaattg ctggtatcct
     5221 ggaactcatc aatcagaaga tgtttgtatc gactccctaa cttttcataa ataaaggggg
     5281 cgtcattttc gccgattacc tgacgcagca tgcttccgat atctgagagt aacaagagat
     5341 tattttcaag tttatacttt tggagatttt tattcatttc ccccattaaa ccataggaat
     5401 aaatgttctt gagcaattca ttagcggtaa taaaagcggt gttaaatttt gaatagtgtt
     5461 ctgctaactg ttttccgatt tcatccaggc ctccatcgac cagttgttta atccgcgcgc
     5521 gtttccttga agattgtgaa aaccaatcct gataaccatt gcaggtttct atgaatgtat
     5581 ccgttaattt aaaatccccg tccagaatct tgatgaatcc attggctacg ctgcgggatc
     5641 cacctttcag atcaataatt tccaaatcaa agtctgaaat tagatccact gctctttttg
     5701 acagagcttt caaatcacga gtataacttt ccttaatgaa aactaaccca tcagccaatt
     5761 tctttaattg ttgaatgtca attttctttc ctgcatcatc aaaattctcg cgaaactttt
     5821 cctgaaataa ttccattccc aattccaaca ggctgtattc gatataccaa cctttatcct
     5881 cgtccaacct gctcaacgca aattccttca gccaattccg agtttcttta tttgtgtgaa
     5941 tttctccaaa tagtctttcg attgcgttct ctacggcgcg ttcattatcc acatcaatat
     6001 catactttac aggttgtttt aattcgtagg caagagctct cactacccgg gtaaaaaaat
     6061 gatcaatagt agaaacatca aaatgcgaat aattgtaaag tattttagtg agcgcttcag
     6121 cagctcgctt ctcaataggc aaatgatcct gcaattccga cttgaaatcg tcttcaatcg
     6181 ctttacgcat ttcactttta ttaccggcag ccagttcttc caatacatca agaatccggt
     6241 ccttcatttc tttggtagcc gcattagtga aagtgaccgc cagcacatgt gaaaaattag
     6301 agggatcttt caggattatt ttcaaatact ctttgacaag cgtaaatgtt tttcccgaac
     6361 cggcagaaga ttgaaagatg gtaagttttc ctccttccgg atgagctttc atgcgcattg
     6421 aaaataaatc aagctgtgaa ggagtttttt tcatagtaaa ccattgctaa attattaaag
     6481 ccgcactcat tacagaaata tattccaact taatataaac tcacaacaga aatggattca
     6541 atacattcca tgccacaatt ttgtttatta aatttgacaa tgtgtgtcac tggttgacaa
     6601 attctccctt attattaccg aatggctaaa agttctacgc gggttgaaat tggcaagcga
     6661 aaattggagc tctccaatct aaaaaaaatc ctatacccaa aggacgggat agtaaaagcc
     6721 caggtgattg agtactatct gaaaatagcg ccaaccattt tgtcgcatat aaaaggacgt
     6781 cctctaacac tagtccgatt tcctgatggt attgaagggg aaagatttta tcaaaaaaac
     6841 cggccggact gggcaccgga ttggattgaa tatgaaacat tgggaaaaga agccaaagac
     6901 tatatgattg ccacggaagc ggcgtcattg gtttggctgg ctaacctggc ttgcctggaa
     6961 cttcaccaaa tgcattccag aagacccaac tttgataagc cggattacat ggtattcgac
     7021 atcgatcctc ctgaaggtta tgaattcacc aaggtggtgg atttggcttt gaggctcaga
     7081 caacacgtgg aaagttatgg gtatgcaaca tttccaaaaa ctacgggggg aaaaggaatt
     7141 catattttat gtccggttga ggtaaaatac agcttcgatg aagttttcca aactgccaag
     7201 ggaattgcag aaccttttgt aaagaagaat gagaaggaaa ctacccttca aatcaggaag
     7261 gaagcaagga aagggcgagt gctgatagat gttttcagaa tccggcaagg gcaatccatc
     7321 atttcgccgt acagtatgcg cggagttcct ggcgctccgg tttccatgcc cctcagttgg
     7381 gaggaaatag aagcaacaaa aaaatcaacc gagtggaata ttaatactgc catggagcgg
     7441 gtattgcagg agggtgatgc ctgggagggt attaatgcgt atgctacttc cctgcatacg
     7501 aagttaaaag gaaagaaaat agtgaagaac cttggaccaa attcaaaata caaaacgcct
     7561 gaaactttag agagttacaa aaagaaaaga gattttagaa aaactcctga acctgatgcc
     7621 aactatgccg gaggagatgg aaacgggttt gtaattcaca ggcaccatgc ttctcatctg
     7681 cattatgatt tacggctcga acaagatggc gttttgaaat cctgggcagt gcctcgagga
     7741 atgccgccgc ggcccggaat caaaaggttg gcagttgcca cagaagatca tccgatgaaa
     7801 taccttaact gggaaggaac gattcccaaa ggacaatacg gtggcggtga tatgtggatt
     7861 tatagtttag gaaagtatga gattgtcaaa gaaaagaaga atgggttcta cttcaatcta
     7921 tacagtaaag acttgaatgg agaattccgg atgcatctga tgaaaaagaa agaatggttg
     7981 ctggaaagag tgaacaatcc gcagattaat tatttacaag atccgataga attcatgctt
     8041 tccacttctg aagaagagcc gccgaaaggt gactattttt atgaagtgaa atgggatggc
     8101 atccgggtaa tgatctcact ggatgagggt gagattagaa ttagaagtcg aagccaaaga
     8161 gatattactg cccagtttcc ggagttgcta attcctgaag aagcttttcg tgctacctgc
     8221 ggactatttg atgcggagat tgtttgcctg gatgaaaaag gccggcctga tttcaagaaa
     8281 gtaattcacc ggctaatgca atctacggag tctggaatca agaaaggtgc ggcaagaaat
     8341 cctgtatatt gttatttctt tgattgcctc tacctcgatg gtcgcccaat tgttaatgag
     8401 ccattatggc gaaggaaaga ttggttgaaa gatgccatta agcccggaac tgcgtacagg
     8461 gtaagtgaaa ttgtggaaga aggggaagaa ctttttgaag ctgcaaaaaa aatggaactg
     8521 gaaggaatta tgtctaagga tcctgagagc cggtatctgc ccgggaaacg aagtaattcc
     8581 tggataaaga tcaaggttag aaatacggtg gattgcgtaa ttgttggata tacggaaggc
     8641 aaaggagatc ggaaaaagta ttttggtgcg ctccaaatca tccaaattaa taatggagat
     8701 cctgtttacc gggggaaagt agggtcaggt tttgattcta aattgatgaa atcaataatg
     8761 gaagaattgg aaaaactaaa aaaaataaaa aatccaaaaa ttgaaaatcc ggttgatccg
     8821 gcggttacca catgggttga gcccaaatta ttttgtgagg ttcaatatgc aatgattacc
     8881 aacaatgaaa ctttccgtga gcctgtattc atcaggatgc gtcctgattt ggaagagtaa
     8941 caaaattgtc aaaaaacatt cgacaaagga aacaggaagt ttaaattttt gcttgcagga
     9001 tcatttcttg cttaaagaga ggaataaaat aatgcccggg tttcccttct ttgcagtctg
     9061 cttccatgat cacatctcta ggatctccgt taaaatttga ttgtatcagc tttcttatta
     9121 cctccttatc tccttcagat aagatcaaaa cacttcttat taattgcgcg tggcccacat
     9181 tgatcttttt ttgcaaaatt tcatttatta aattccatac aattaaattt tcttcattgc
     9241 caggaaataa ttcattgatt ttcttcacga tgtcttcagg cagcttcatt ccaaattaaa
     9301 aatcaatatc gtgtttttta caatgccatc ttggtgaatg gatatcaaca aagcagccac
     9361 caagcttaat aattccctgc tctgcttttt ttaaagatgc tgaagtcggt tcgccatata
     9421 caattgggat catcctgttg ctatggtttt tagcttcctt agggcaattt tttagaatta
     9481 tctggttttg aatttgatta cctggatcga tcattatata gcttattgta tcgtctggtt
     9541 ttccagagat gtctgtttgt tctttaacat ttgaattgct tcggggctgc tcgcaggacc
     9601 tcgcaaatcc gtgtagtccg cgggagaaag ttttatataa tcccgctgat atcgcagatc
     9661 accgcagaga aaaatggtta gagagcattc tgcgcaaatc cgcgtaatct gcgggagaat
     9721 tttttttaat cccactgata ttgcggatta ccgcagaaat gattcacgaa gctttcatca
     9781 gtatcagcgc ggtttgtaac aacaggaatt tcacactcac ttccaacatt ggtataactt
     9841 gttcaattaa aatgtgtcct tgctgttgta aaagaagcgt tcatatcgac cccaatcgcg
     9901 ctgaatagtc tgcaaatacc gaaattccct tttgccgcct gcctgtaaag gaatcaataa
     9961 cgtacatcgg accggatgta gaaataataa gaaatgaaca taatgaaaag aaaagcgaag
    10021 tgaatgagca caatcggtct atcgcggaaa aatgggattt ttttatgaaa caatatcagc
    10081 ccgcagcaga aaattccgat cagtgcccca aagagaagaa aataaaaagc ataaccgatg
    10141 gctcctgctt ctttaaaata tcgggcttca aaaattgaat ccagcataat tcccaaggta
    10201 acgctggcaa ccgggaaaaa taaaagggtt gcgatgatga tgaatatctt ttccagcatt
    10261 taattttact attaaaaatc aagcagtaat caccgctggt aacgactgcg ccataggtaa
    10321 agtgaaatag aatgagcttc cctttttctc agtgctacgc acccaaattg ttcccccatt
    10381 tttctcaacg aattcacgac aaagaattaa tcccagacct gagccttttt catttgccgt
    10441 acccctcgtg gagaaatttt ttgtaccgaa taatttactt aactggtcat gaggtattcc
    10501 aattcccgta tcacggatgg agattgtaac ataagatgca ttcagctcac aattaatgat
    10561 tacttcgccc atgttttggg tgaatttaat ggcatttgaa agtaagtttc gtaccaccag
    10621 ctcaatcata tttcgattac cataaataat tgccgaattt gataattgat ccactaaacg
    10681 aatgtttttt tgatcagcca tgggcttgag taaattaatc gcattttgaa caatctcatt
    10741 taggttcatg ttctcaaagg caaccgggtc tccgcttaaa tgctctcttg cccagtttag
    10801 caagttatcc agcaaatcca ttacatgctg ggtgttatcg ccaatcgagg acaaaagcac
    10861 cgttagttct tccttggtaa tgcgttggtt tgccgctaat tccaggatgc ttttgaggct
    10921 gataagaggg tttctcaaat catgggaaat aatggaaaac aatttatcat ttaaccggtt
    10981 aagttcttcc gaatatttct tttgttcctc cagtttacgg ttagtctttt gtttctgccg
    11041 aagaaaatac atcaagccag acatgaccgc gaacatggtg agcaagacac aagcgaatat
    11101 aaccagttga ttttttctct ggctccggtg aagttctgtt tctttattga gcaaaacgat
    11161 ttcctgatct tttcgctcca ggtcaaaagc agcctgcatt tcggcaatct gtttgctttt
    11221 ttgagtgtcg aatatgcttt cttttttggc tacatatttc ttgtgccatt caaaagctcc
    11281 cttcatgttg ttccgggcag aatccagacg ggaaaattcc aggtaattat caatcagtgc
    11341 ttcttccgac cctgtttccc gggcaagtag ttgcccgtaa agcaaatgtt tttcagacga
    11401 ggagtattgc cggaggctga tcattacact tgcgatttga ttgtgggctt caatcaaagc
    11461 ctgcttatca ttgatctcct ccattatcgc tttagcctgc agtaacgact caagggcttt
    11521 ggaaaattgt ttattctttt tatagatcct tgcaatgttc cctaaggtgt aggctatacc
    11581 ctgctgatga ttgatctccc tgaaaagaac atacgcctgc tgataataat ccagcgcctt
    11641 gtcaacttcc tgaagctcca tgtaattatt agcgatatta ttaaaagaaa aagctatgcc
    11701 ccggctatca tttagtttcc cttttatcgc aagagattta aaatgataat caagcgctgt
    11761 ttcatattgt ttttgatcaa agtatatgtt ggcaatgtta tagtaggaaa aagcagtgcc
    11821 ttgctcatca ccgatttctt cccgaagcct gagggatttc agatagtatt ccaaagattt
    11881 ttcaaactgg cttaacttgt aataggaatt tcccgtccaa tgaaaaacaa gcgccaggtc
    11941 agactttcgt ccaacttttt caaagtaggg aattgcttcc agcaggcttg tcaacgaccc
    12001 tctaaaatag gacttcctgt aattgatgat acctatgtgc attaatgcca ttccggttcc
    12061 tatgtcatag tcaatatttt gggacaatga atacgctgtt tcagcttcct tcattgcttc
    12121 ctcatcatta tcatttctcc acaatgcctc aatcagctca atggaaacca gaatgcggcc
    12181 ggtatcggat gaaagtgaat ctctaactgt tttcaggtca ttgatgtttt caggttgtga
    12241 aaaggcaggc cacgccagaa ccaacaaaaa agttattttc aagaacggtt tgatcatatt
    12301 tctattcagg ttgggattga aatcctttca tttatatttc gatataaata acttgcatcc
    12361 ctttatcacc cggcttataa tcgggacggg catcattaaa attttcatcc aatatttgaa
    12421 tcgatccgat ttttgagagg tgaaacaact tccatccggg caatccggtt gaactattac
    12481 cacccacctg acatgccctc aaggcttcat cgcctttcac tgtattacaa taacaatggg
    12541 gctcaacaac cctggttagg tcattgtaat tgaatcgcac caattttttg tttgagattg
    12601 cattgatgat caggtgtttc atggaagcta gaaactatcc ccaaacctag ttttttagca
    12661 caccctgggc tggtaatatc ttcccaattt cgggtaatca tgtaacattt tgagtgaagt
    12721 ctgctaattg cagccagggt tttgactctc cccaatttat tgaagccaat cagtgtttcg
    12781 tcaaaataag cctcaacgcc ccggtcgtct cccaaaagca ttttctcttt cacaactgta
    12841 atttcatgct ggctgactat tttttgaaac gatacgggat aagagttgag ggaagatttg
    12901 gttgatggtg cgtggcaaga cattacatct ccacccatga attttatttc caaagcattc
    12961 tttacgattc gggcttgctt aactttttgg atgatactct tcagaacttt ggtgtatccg
    13021 ggagaatcag ccaggtagga gaaatgcttt aataatgctt tcttcagatc ctttgcgcta
    13081 acaatggttt tccatttatt gatgtaatca cctctgtatt tggtttcata ttctgttttt
    13141 attagccgat taaacctttg atgtggtttt ttgttcagac atttttccaa acttctattt
    13201 gccagaattt gtctgacttg ctgtctttgt aagtgaggtt gcgcttcatt tcatccttat
    13261 tcatcgtata tgcatggttt gttttattct atactttcca gtcaccgtta ctcattattt
    13321 tatgttaggg caacaaaagt tttttatgct taccttctgt aagctcaaac ggttgcttac
    13381 cgtctttatc tttcaatttt ttatcggctc ctttttccaa taaaaattta atcactgcaa
    13441 tgtttttatc gttaggccgc atggctgctg cgtgcagaat agtgtagccc cactcgtttt
    13501 tactatttat atccatatcg ttttcaagaa aaaaatcgat tatttcccgg ttgcccctgt
    13561 agccagcctc tgtaaagcaa ttggtttttt ccatttcaga aatgtgaaac gcctccaata
    13621 agtggaagaa gattatggag tcattttgaa cggatatgaa aaataatttc ggtgtcttct
    13681 gggcaccaaa ttttataaat aatttgatgg tacttaatgg taaactacca actgcaagat
    13741 caaatgaagt gaagccccca ttgtcagcaa tgttggggtc agctcctttc gtaagaagca
    13801 ggtccaccag ggttgtttgc gaagaagcta cggcccgcga tagaactgaa gaagtgttcg
    13861 ggtcagcgcc ttcatttata agtttagtaa ccttatgcca gtttcgtttt actaccgcct
    13921 cttcaagctc cttatctatc tcttgctttg attggggaac tgcaatggga aaactactga
    13981 cgagcctttc aaattcttcg tgaaattttt tccgaaaaaa atcatagtat ttgttagatt
    14041 ctttttgccc aagcttcagt tcatcaacta aatgttccgc accactcata atgaagttct
    14101 cccaaagact atccgcgaac tcacccatat caccatcagt atctagctca tctttaacct
    14161 ctccctcaaa atcgctaact gactttttaa tataattttc tattcgcgtg aaatctgcag
    14221 gtttcattgt taagcctgct ttcttcttag gcgctggtga aggggctaca actttagtag
    14281 ctcctttact ttccttatat ccttttctta gcttttcatt caacagtttt tctgcttcct
    14341 taaaacattt ttcttcgcta tcaaaacttt tggtttgtga ggtcccagca gttccagttt
    14401 ttccgtaggt aacggtaaat gagtttccgg caacttctat ttgccagaat ttatctgact
    14461 tgctgtcttt gtagacaaga ttttttttca ttttaattaa catttaaaat tgtttggttg
    14521 ttaaaaatat ctttggtttc tgattctcag aaaccagatt tcaaaaaatt catctgaaat
    14581 aaatgaacat tatttcataa ttacagacaa cagctattcc tcctcctcat tttcaccaca
    14641 gatttcaatc tgcaattccg taagtgtttg tttcaattcc tggaggcgtc gcaccgcgtc
    14701 tcgctcccct ccgaggcaga gccgaaagtt gttgccgatt cggcgcacag caagccaggc
    14761 gcctcgctct tcgatgcgcc cgactaccca tcctgcctcg aacgtcgccc cagccttcag
    14821 ccccagcccc cggtcgagga cggtcgccat ttcgtccaat gaataattcc agtccaagga
    14881 catcacctca tcgccgacgg ggtacacgat cgacgtgaac ggccatgccc agcgccaggg
    14941 cagcacgcga tcgaacgtgt atccaagacg attcgcccgc catgattccc cttcttcgag
    15001 ctctgtaatg aaacactcgg gctcattggc cacggtatcg ttctcaccca aacgcgcgaa
    15061 gaccaagggg tcggaatcca ccagttgctc gttccgtgca atcgcgacca gacgaatata
    15121 gccagtgtca gtgcgagcgt gccggacgat aggtggaacg ccaaccacgg cgcgcaggat
    15181 cgcatacgac gattgctctt caattgcagc ggcagcaccg agatgatcgg cgagtatcgt
    15241 cttgagcccg gcagggagcc aagcctgttc gatctgctcg cgcgacacgg gcgcctcaag
    15301 gccggacccg aggacagcgg tagtccgttc cagcatccgc tcgagcaacc accagccagg
    15361 atgggtcacc atcacggata atcggattta atattggaaa ttgacgtgta ccccacacta
    15421 ctgcataatt gcttcgctct cttttaaaaa tcgcctatac ttcgaacggc aactcatact
    15481 ccttaactac cagtttcgaa ctcctcgttt tcacctccac ttttgccttt agcgggctgc
    15541 ctgtaaccag gtaaatttta atataccggt cggtgtgtac catccatgat ttgtctgata
    15601 acaggtaatc cgttactcct tctttgccgg ccttatatgc ccactgctcc acaacattcg
    15661 gttcgaagtt cacaatattg cccatgtaaa taatctggat gccgggcttt gagttgtttc
    15721 caagttcagt gcagctctcc actataatgt tcaaggagga gttgtcttca aggtctttta
    15781 cagaccattc atcgatacga gttttaatgt ttgtcataat tctctttatt ttttgaaatt
    15841 caattggtaa aaaaatccgt gtattcataa aaaaaacagg ctaaaattcc ggtgatttaa
    15901 tcgcccgatc tccctttcaa taagacttgc catcgctaaa gccacttctt cccgccaggg
    15961 cggctgaaat tgtcaaagat gattcgattg aacccacgcc taaatgtgca ttcaggttaa
    16021 cagctacacc aattatgcta actgttgaat atttaacaaa ctccctgcga ttgatcttca
    16081 tttttgatac agaaattata actacacaaa tcctttcttt tgaaccgttt aatttcctgt
    16141 tagcattttg ggttgctgct gagtcataca atgaatacct cctccattag agttaaggtc
    16201 ttcagctttg tggatttgaa ttatttctct accgggaaag gcttgctgaa gaatttcttt
    16261 tgcttgctcg tctttttgtc tggtggataa gggtctgcct tctttatagt aggccgcaac
    16321 taaaacgcat ccgtttgtaa ctaaaaagtt gagataactt gacgctatca attgattgat
    16381 tgtatctccc tcgagtacag gtgtaccatc gaaataacgg agatcatcat accatgacaa
    16441 ttcgtaccat ggtatttctt tactatatgc taaggataca taatagtgat cagtgaccgg
    16501 aattcgaatg atgttaaatg gattcccgtc ttgatccgtt tcttttttca acatttggta
    16561 attctcttca agtatgtcgt aagtcatgct cataatctca tttgactgac gctcttcttc
    16621 acttacctgt gctaatatta ttgtgttagg atcagcaaac cgggcatatt catctatatg
    16681 gccacaggtg gtagatgtaa atgcgtttcc gggtaatgca cctttcattt tcatctgatc
    16741 cccttcaata ggaccagttc gcagccacaa cactttcttt tgccccaata ctcgtttcaa
    16801 ttcttcagca atttgctctt tcgtcatatc cgggttccgt tgaagctcaa ctgattcagt
    16861 gaccataaca gttcccattc cattaaaatc cctgtctcca ccttcgctta tcatacttga
    16921 cctgatcgca gggatgttaa gaatcctggc gaatgatctg tcggtaacgt ctatatcgat
    16981 atttatagga tcttcctgtt ttcctacacc ccaggaatta aatccaaaat caacaatttt
    17041 atattgacct tcattatttc ggacaaaaac agggccgttg tcacgcaacc agcgatccgt
    17101 acagttaggg ttaataaaga attctacatg actatatggg attccttcct ctttcaggat
    17161 tcttttgacc tcactctcct cttccttatc tgaaactacc agcttaatct ttacaaaagg
    17221 attcaaggct ttgatgacag ctttgattac gccatcagtt gaattgccaa gataggtttc
    17281 agttgtccat ttaatccata ccgcctcgtg ttcttcaaac tctgccggaa atgtataaaa
    17341 agaagtgtca gatacctccg ctgcggagac cgctttactt tcttcaagcg gcacacatgc
    17401 atagaggcta atcgcggata acagacaata gattaatggt tttttaagat ttttcataat
    17461 aattataatt aggacttttt tagtttatta ataccccggg ttttgctgta atgtcaggcc
    17521 caaaccggaa gcagttagaa tttcaacaaa gggaattggg aatatcgatc taaaattttc
    17581 aatgccataa acctcttcca ttttctgatg tctggccaga tcgaagtagc gatgcccttc
    17641 ggcatagaac tctcttcttt tttcctccaa aagaatattg atgaagtcag gcatattctg
    17701 cagatctccc ggttgcaatg cggataagcc ggcccgctcc ctgatcacat ttaaatccgc
    17761 tattcctgcg ttcggatcat ttaacaggat agcatttgct tccgcacgga tagtgtacat
    17821 ttctgccaac ctgataatga aggctgatgc gtcttgagtt atgtactttt cagttgttaa
    17881 caggttctca ttatcaaaat cgggaaatat tgtagctctt cttgtatcgt tctcgtcaaa
    17941 aatatcgaaa aaatcgggag agattgtcgc ttcatccctt tcaccaaact ccctcaaata
    18001 ataacttggg tcttgtgcgc taaaattcag ctcgaagatt gattcggagg aatttttttg
    18061 agagaaaaca cttgcatagt tttcttgcag agcataattt cctgatgtga tgactgcgga
    18121 agcatactct gaagcttttt cataatcctg caaatacaaa taggtacggg caagcaacgc
    18181 ggcaactgtt tgtttgttca tataagccgg atgataagca tccggtaatt tgctttctgc
    18241 gttttgcaga tcagtaatga tttgctgata ggtttgtgct accgtacttc tgggagtaaa
    18301 ctccgcatta aaagcaggct caattcgaat gggcactcca aaggacgagc cggagtccca
    18361 gtgttctccg aaggttctta gaagatcgaa gtaaaccatc cctctgatcg tctcggctgc
    18421 acccactatt tcattcttct catcctgggt gaaaagcggg tcatccactc cgggaacata
    18481 gtagataata ttattagcaa cattgatggt cctgtacatt tgaagccaga tcaatttgat
    18541 gttttcgctg gtggccggta cgtaatgact gtcaaggtgt ttgtaactat cccagaatgc
    18601 cgagatcacc aggtcttctg tgaacatatc cacgctgata ggaaaatacg ctccataata
    18661 gtcaatctcc tggagtgcat cataaagtcc gttcaaggca ccttgagcag aaaatttgtc
    18721 agtgataggg atctgaccat caaactgatt tttgggttcc tggtcaagaa cgttacatcc
    18781 gttgaaaagg atcaaaaaaa ccagtataga gagtttaaag atgtatctgt tttttattga
    18841 tttcattgta ttatttctaa aggttattgt ttgatttttt ttcttaatga aaattatcta
    18901 aaatccaata ttgaagcccg ccaggatagt cctcatttgc cccagaccgg aatagtcctg
    18961 accctgggtt agtaaatttg agctggtttc aatttcagga tcaaacccgg tgtatttggt
    19021 gaaagtgaga aggttttgtg cttgcacaaa aatcctgatt gatttgaata cattcgttct
    19081 ttgcaatagt gaattaggca atgtgtacga taattcaata tttttaagcc tgagataaga
    19141 accgtcttcc aaccactggt cactaaagtc ctgataattc tttgtcttta ccgacgccct
    19201 tggaatatcc gtaatgtcac cgggttgtct ccaacgatcc attacagata tcgagttatt
    19261 cccatttgga agaatataaa catccccgtt tgcataatct tcatcactga agtacaggta
    19321 attgtatcct aattgttcaa tttgggtttt gcttcggttg aaaattttat ttcctacatt
    19381 gaattggaag aagaaatcaa gattgaaatt tttatatgac aacgtattgg ataatccacc
    19441 gaaaaagtct ggctgggcgg tacctacaac ctgggaatca tcgctgttaa ttactccatt
    19501 cccgtcaatg tcttcaaaca ttgcatcacc tgtttgcgga tcgactccca ggaaatggat
    19561 catataaaat gtaccgaggg gcataccctc cattaaaata tgagattgat cctgaggttc
    19621 tccgtcattc aaaacctcca ccacttcatt acgaataaat gaaatgttga agcctgaact
    19681 ccatgttaat gccctgtcca ggtttactga attgagagcg atctccaatc ctttgtttct
    19741 cagctttccg gcattttttg tcataacact ggttccagat gtcccgggaa caggaacact
    19801 caacaataga tctttagtat tgctcacaaa tgcatccatt attaagccta ttttcccatg
    19861 aaagacttcg atatcaattc caaggtttgt ttgggtagtt ttttcccacg taagatccgg
    19921 gttggcaatc tggctcactc cctttttcga aggagacatt ccaccttgtc cgttatatga
    19981 ggcattcaat gcccataaat accgggaagc aaaattggaa atgccttcct gattgcctgt
    20041 taagccatga ctaaatctta atttaaggtc gctgaataaa ttactttctt tcataaatcc
    20101 ttcttcactg attctccagg caactgaccc ggatgggaaa attccgaatt tgttgttaat
    20161 tccaaaacgg gaagagccat cagccctggt ggtcagggta agtaaatatt tgcccttaaa
    20221 tgcataattc aatcttgcaa aataggattc gatgccccag gagtatccac cactataccc
    20281 taccgttaca cttgaggctg aacccagttt tccttctccg tccattggaa attggcgagc
    20341 ccgaatatct gaatcatctc tttgcgattc ctgagcagag taaccaagca ctgcattaaa
    20401 ccgatggcta cttccagcag aatagtcaaa agttaaagtg ttttcactca gccaggtaag
    20461 atcctgatag gttgagtatt gtcctaatcc gtttgctccc gcccggattg attcccaggt
    20521 tagagatgat atatattgtt catctttgag gtccgtgtag tcaaccgacc atgcagtttt
    20581 gaactttagc caggaggtta gtttaacttc gccgaatata gtacccagga gcctgcttgt
    20641 attagtgtat cgattatatt cgttggcagc cttcacggga ttttcaagca tccaccacga
    20701 accgtaattg aagggatcgg cgtaagaacc atctgcctcg taaacgggca tggtaggatc
    20761 gcctgcctgg gcatttacga ccacgccata aaatgatgca tcagtttctg tcctcttgag
    20821 tattgatctt gaaattccca gactggttcc gatagtcaac ctgtcagaag catcaaaatc
    20881 aagatttagt ctcccgttaa atcgcttgaa gcctgcacca atctgcacac cttcctggtc
    20941 aaaataagta agaccggtat aatatcgtat attgttactt cctccctgag cgctaaattg
    21001 cgtttgacag atgggagccg ggttcattat ttcatcgtac cagtttgtgt tttgaagctc
    21061 tggatcgttg atatcaaggc ccatttgatt agtataaaaa ttttccccta aaccatcgtt
    21121 tgcactggct tcattcataa gttccagaaa ttctctgctg tccatcattt caatcatatt
    21181 ggtagggccg agttgaactc cggagtaatg atttaaactc atgattcctt tgccttcctt
    21241 acctctttta gtagtaatga gaacaacgcc atttgcgcct cgggatccat agatcgctgt
    21301 tgcggacgca tcctttagga tttctattga agcaatatcc gctggattaa tatccgtaat
    21361 tgaattgctt ttggtgttca ggtaatcggg gaagtcttc
//
LOCUS       DASWHX010000205         6758 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_595257, whole genome shotgun sequence.
ACCESSION   DASWHX010000205 DASWHX010000000
VERSION     DASWHX010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 6758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6758
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_595257"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(2..74)
                     /locus_tag="VGA21_13575"
     tRNA            complement(2..74)
                     /locus_tag="VGA21_13575"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(39..41),aa:Met,seq:cat)
     gene            complement(190..2793)
                     /locus_tag="VGA21_13580"
     CDS             complement(190..2793)
                     /locus_tag="VGA21_13580"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX9651588.1"
                     /translation="MRETNNTSPPKRLRQVLEWLYGSDTVEDLIGDLDEIFYRNSELG
                     KKTAAWEYNKQILSLLFSYGVKRRKRMQKKKYVESNYYIMHKSYLNLLVRSLRKDKLF
                     SIIRLSNLVIGIAVSLLCWIYVRYELSYEKSFQNSEQIYRLQHQTPDKIWAAVPRAAG
                     VYASENFQAVISMVRFSPASTLVRLDEKVFMEDEILFSDSTVFKVFDYEFVAGSPDKS
                     LTNPHSVVLTESSAKKYFGNNEAVGKQLLFEFDRGEPREVTGVIKDPPSNSHLQFDFL
                     ASIYFENPEYNRSWSNWGTYAYLLMKPGSDLNSIGAGIEAEYIKQYKMEDDSSLKTIF
                     MPLEKIHLYSNGEKELSVNANINYLYIFSFAGFFVLIISMLNFINLTLAKSSDRGREV
                     GIRKSIGASKGSLAVQFLSESYLQVIISSLLGISLVYLILPFFRELSGLPLPFSLWQP
                     DIILPVVILVLVAGLICGIYPSMIISKFKPAEILKSNMSNAGTKTGDTRQILSIVQFV
                     ISAILIIGSIIIYRQSQFIRERDLGFEKDQVITTRLNYSMNVKLDVFESELKRVPGII
                     EVSHSSSIPGYRIMREGVTDVSSGISPTNRLLLTSESFIESFDLQIIKGRGFTKNSNE
                     PKGEWILNEEAVKAYFEVEEVDPIGRNLAWGGDTAEVVGIVKNFNFETLHEEVSPLAI
                     YREPRARSYLAIRFEAGKIEGVLRNINKVYGEVYSNLPKAEFEFLSHRFETLYNAENK
                     LQTLIFIFCALSLLLTISGIFALAVHYTKKKTKEIAIRKVLGSSVLNVIILISRHFAI
                     MVIISFVISIPLSYYLTNWWLEQFAYKINMGIITFITGGFSILLIVLLSSLYTTINTA
                     ITNPVKWLRNE"
     gene            complement(2790..3122)
                     /locus_tag="VGA21_13585"
     CDS             complement(2790..3122)
                     /locus_tag="VGA21_13585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020530725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX9651589.1"
                     /translation="MKGFIGEFEELVLLTVAVLGDNAYGVLIKDEIEKTANRGISLGA
                     LHATLGRLEDKGFIESHMGGATKVRGGRRKRYFRLTRQGKIALHTIRNLRDKLWENSK
                     TDLSLKEI"
     gene            3261..3710
                     /locus_tag="VGA21_13590"
     CDS             3261..3710
                     /locus_tag="VGA21_13590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010855621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2784 domain-containing protein"
                     /protein_id="HEX9651590.1"
                     /translation="MGTLAGAFYDKGKFHYLRISMPTALYQFADFFFFAFHSALILFN
                     SLGWLFQKTRFYNLITLSLTAFSWFILGIWYGWGYCFCTDWHWNVREKLGFIDHTNSY
                     VDLLLIKITGIDFPNSLVDTATVTVFTVSLIMSVWLNVRDFRNKNHK"
     gene            complement(3743..3946)
                     /locus_tag="VGA21_13595"
     CDS             complement(3743..3946)
                     /locus_tag="VGA21_13595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651591.1"
                     /translation="MENIMQKSCSKIDARDVIANNLSFLSKEKRIWLHRTADYNELGR
                     QDEEILFIMKDDVTMLSHSVFAY"
     gene            4113..4754
                     /locus_tag="VGA21_13600"
     CDS             4113..4754
                     /locus_tag="VGA21_13600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651592.1"
                     /translation="MKKILYFFFLITLVPFLSYSQQRQQGSGDISLSVNLVTSSLPMK
                     MKEYKDENLIPRFFEFNFEKWFKNLSLTGSVRTFEASKLKWAYRVSSQPLNIDHISTN
                     FELRRVELNLFLGARVNISSKFQVQVQTGIINSAGNEIVDVLQYAIANGILMSGEVHS
                     SKNKTYYDFGGQANLLILFKVSQLVQINLFGGSGMFVDGRTRIEGGVGTKFSF"
     gene            4793..4915
                     /locus_tag="VGA21_13605"
     CDS             4793..4915
                     /locus_tag="VGA21_13605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651593.1"
                     /translation="MSERFFSNDSLLILNEAREVIQYDRLIILEENQKKGKSNQ"
     gene            complement(4909..5487)
                     /locus_tag="VGA21_13610"
     CDS             complement(4909..5487)
                     /locus_tag="VGA21_13610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651594.1"
                     /translation="MKNKAFLLALSLSTACNFTGVFVPDPIDPRLPMYTETGNDVAGA
                     FVNKIVWKSIATSETGFISPGYDFGFPSIYFYDSGDRIKLKLTGKLSGVETDLIFDIL
                     TSNISSPEDFARLNGSKIILDGVNNKGYLRSSRYQLNSSSGVGQIYFKKINVSGQRIS
                     IAGTFGFLVSDPIHGNLDVSYGRFDYGECEVY"
     gene            complement(5477..5989)
                     /locus_tag="VGA21_13615"
     CDS             complement(5477..5989)
                     /locus_tag="VGA21_13615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651595.1"
                     /translation="MIKQQNLVGSVKGTPGIHLGAAMNFKPLRKSSKFSLEKQSALIT
                     RGYTQTIGSFKHEASLAYITSTLLAKYSFRFLSIQNGFSYEVLADASGPQFEAKYSEF
                     DISWISGINLFENRRAGIYFHYFLGLKPVYNYYMFDKLGNFIEEIHDVKNRSLLFGIK
                     VRITEIYDEK"
BASE COUNT         2105 a         1369 c         1139 g         2145 t
ORIGIN      
        1 gtagcggggg taggacttga acctacgacc ttcgggttat gagcccgacg agctaccaac
       61 tgctccaccc cgcgatatga ggcacaaaat tagttcactt atcgcttaaa accaaactgc
      121 gccgattacc ggaattaact atcatcgccc gtgtcttcct aaacaacatt cattggctgc
      181 tcccaatgtc tattcattcc tgagccattt caccggattt gtaatcgcgg tgttgatggt
      241 tgtgtacagt gaacttaaca gcacaatcaa caggatggaa aaaccaccgg ttataaaagt
      301 aattataccc atatttattt tataagcaaa ctgctccagc caccagtttg ttaaataata
      361 ggacagcgga atcgaaatca caaaacttat aataaccata atagcaaaat gcctgcttat
      421 tagtattatc acattcaaca ctgaacttcc caaaactttc cttatcgcga tttctttggt
      481 tttctttttt gtatagtgga ctgccaacgc gaaaattcct gaaatggtga gcaaaaggga
      541 cagcgcacaa aagatgaaga tcaaagtttg caatttattt tccgcattat ataaggtttc
      601 aaaacggtga cttagaaatt caaactcagc tttgggaagg ttagaataaa cttcaccata
      661 caccttatta atgtttctca atactccttc aattttaccg gcttcgaaac gaattgccag
      721 gtaacttctc gccctcggtt cccggtatat agcaagtgga ctcacttctt cgtgcagggt
      781 ttcaaagttg aaattcttca caattcccac aacctcggcg gtatcacctc cccaggcgag
      841 atttctgccg atagggtcaa cttcttccac ttcaaaataa gccttaacag cttcttcatt
      901 taatatccat tcgcctttgg gctcgttgga attttttgta aacccccggc ctttgataat
      961 ctgcagatca aatgattcga taaatgattc gctcgtaagc aacagcctgt tggtcggaga
     1021 tattcctgaa cttacatcag taactccttc ccgcataatt ctgtaacccg gaattgaaga
     1081 ggagtgtgat acttcaataa taccaggaac gcgcttcagt tcactttcga atacatccag
     1141 ctttacattc atggagtagt tcagccgggt agtaattacc tggtcttttt caaaacccaa
     1201 atccctctcc cttataaatt gagattgcct gtaaattatt atggagccaa ttattaaaat
     1261 cgcggaaatc acaaactgaa caatagaaag aatttgcctg gtgtcgccgg tttttgtacc
     1321 tgcattactc atgtttgatt ttagaatttc ggcaggctta aactttgaaa taatcataga
     1381 agggtaaatt ccgcaaatta atcctgccac caacaccaga attactaccg gaagtattat
     1441 atccggctgc cacaatgaaa aaggcaaagg caaccctgat agttctctga agaatgggag
     1501 aattaaataa acaaggctta tccctaataa agaagaaatt ataacctgaa gatatgactc
     1561 ggaaagaaat tgaacagcca ggctcccttt tgaagctcca atcgactttc ttattcccac
     1621 ctcccggcct ctgtccgaag atttagccag ggtaagatta atgaaattaa gcattgaaat
     1681 tatgaggaca aaaaatcccg caaatgaaaa aatgtacaga taattaatat ttgcatttac
     1741 tgacaattcc ttttcaccat tagaatacaa gtggattttt tccaggggca tgaaaattgt
     1801 ttttaaactg gaatcatctt ccatcttata ttgttttatg tattcagcct caattccggc
     1861 tccaatggaa ttcaaatccg aaccgggttt catcaaaagg tatgcataag ttccccaatt
     1921 tgaccaactt cggttgtact ccggattctc aaaataaata gaggcaagaa aatcaaattg
     1981 aagatgagaa ttcgaaggag gatctttaat tactcccgta acttctcttg gttcaccacg
     2041 gtcaaattca aaaagcaatt gtttccctac cgcttcatta tttccaaaat atttttttgc
     2101 agaagattca gttaaaacta ccgaatgtgg attagtaagc gacttatccg gacttccagc
     2161 tacgaactcg taatcgaaaa ccttaaaaac tgttgaatcg gaaaataaaa tttcatcttc
     2221 cataaaaacc ttctcatcaa gccgaaccag ggttgatgcc gggctaaagc ggaccatgga
     2281 aattactgcc tgaaaattct cggatgcgta aacgcctgca gccctcggca ctgcagccca
     2341 tattttgtcg ggtgtttggt gttgcagccg ataaatttgt tctgaattct gaaaggattt
     2401 ctcataactc agctcatagc ggacataaat ccagcagagc aaagaaactg caattccaat
     2461 tacaagatta ctgagtctta ttattgaaaa aagtttatcc tttctcaacg atcttactaa
     2521 gagattcaaa tagcttttat gcatgatgta atagtttgat tcgacatatt tttttttctg
     2581 catccgtttt cgtcttttta caccatacga aaacagcaaa ctcaaaattt gtttgttata
     2641 ttcccacgcc gcggtttttt ttcctaattc tgaattccta tagaatatct cgtccagatc
     2701 cccaattaaa tcctctactg tgtctgaacc ataaagccat tctaaaacct gtcttaacct
     2761 tttcggcgga gatgtgttat tcgtttctct catatttctt ttaatgagag atctgttttt
     2821 gagttttccc aaagtttatc tctcaaattc ctgatagtat gaagagcaat cttaccctga
     2881 cgtgtcaatc taaaatatct ttttcttcga ccgccgcgca cttttgtggc tcctcccata
     2941 tgtgattcaa taaaaccttt atcttcaagc ctgccaagag tagcatgaag cgctcccaga
     3001 ctgatgccgc gattggctgt tttttcaatt tcatccttta ttaaaacccc ataggcatta
     3061 tcaccaagca ccgcaaccgt tagaagcact aattcttcaa attcaccgat aaatcctttc
     3121 ataatccttg atacgaggat ttgggctatt tgtttctatt tttaatataa aataagagat
     3181 ctttcaggaa aggcaacatc ctgagacaaa tagtaattta ttgttttgtt tcgcgttttg
     3241 cgaaatgcga aacccacccc atggggaccc tagctggcgc attctatgat aaaggcaaat
     3301 ttcactacct tagaatatca atgcctacag cgctctacca atttgcagac tttttctttt
     3361 tcgcgtttca ttcagcgctt attttattca actctttggg ctggcttttt caaaaaactc
     3421 gtttttacaa tttaattact ctctccctta ctgcattttc ctggttcatt cttggaattt
     3481 ggtatggttg gggatattgt ttttgcactg actggcattg gaatgtccgg gaaaaacttg
     3541 ggttcattga ccacacaaat tcctatgttg atctactgct cataaaaata acgggtatag
     3601 acttccctaa ttccctggtt gataccgcta ctgtaactgt attcaccgta agtcttatca
     3661 tgagtgtttg gttgaatgtc cgcgatttca ggaacaagaa tcataaataa ttaatcttcc
     3721 gtctaaattg gtattcgttt cttcagtaag cgaaaacact gtgagataac atggtgacat
     3781 catccttcat aatgaataag atttcctcat cttgcctccc gagttcattg taatctgctg
     3841 tccggtgaag ccaaatgcgt ttttctttgg aaaggaaact caagttattc gcaataacat
     3901 cccttgcatc tatcttcgaa caactctttt gcataatgtt ttccatttta gaagtaaaat
     3961 ccgggtaaat ttactaccta acattgactt taaagagtca agttattaga ttctggtatc
     4021 gcttgttggt cgaaaaaaca ccaacatcgg agaggtatag ttttcttcat ttttcattaa
     4081 ctttgattaa cgttgcttaa ccccattagt taatgaagaa gattctatac ttcttttttc
     4141 tgattacact tgttcctttt ttaagctact cccagcaaag acaacaaggg tctggggata
     4201 tttctctttc cgttaatctt gtcacttcct cattgcctat gaaaatgaag gaatataaag
     4261 atgaaaatct cattcccagg ttttttgaat ttaattttga aaaatggttt aagaatctct
     4321 ctttaacagg aagtgtaaga acatttgagg ctagcaaatt aaaatgggct tacagagttt
     4381 catcccaacc tctaaatatt gatcacatct caaccaattt tgagcttcgt agagttgaac
     4441 taaatctgtt ccttggtgca agagtaaata tatcttctaa atttcaagtt caagtacaga
     4501 ccggaattat caattcggca ggtaatgaaa tcgtggatgt ccttcaatat gctatcgcta
     4561 atggtatttt gatgagtgga gaagttcatt catcaaaaaa taaaacatat tacgattttg
     4621 gtggtcaagc caaccttttg attttattta aagttagcca actggttcag attaacttat
     4681 ttggtggatc cggaatgttc gttgatggcc ggacaagaat tgaaggaggt gtaggcacca
     4741 agtttagctt ttgaattgtt gaaagctgag cgcagggata aaacagtaaa aaatgagtga
     4801 gagattcttt tccaatgatt ctttgctgat acttaatgaa gccagggagg ttatccaata
     4861 tgaccgtttg attattcttg aagaaaacca aaagaagggg aaatcgaatc agtaaacttc
     4921 acattcaccg taatcaaatc ttccatacga tacatccaaa ttaccatgta taggatcact
     4981 aacaagaaac ccaaatgtcc ccgcaataga tattctttgt ccgctcacat taattttctt
     5041 gaaataaatt tgaccaaccc cgctgcttga attcaattga tatctggatg aacgcagata
     5101 tcctttatta ttgacaccat ctaaaataat ttttgaacca tttaaccttg caaagtcttc
     5161 tggagaggaa atattggaag tcagtatatc aaatattaaa tcggtttcga caccagataa
     5221 tttacctgtg agttttagtt taatcctatc gccggaatca taaaagtaaa ttgatggaaa
     5281 cccgaaatca taacctggac taataaatcc ggtttctgaa gtagcaatac ttttccaaac
     5341 gattttattc acaaaggcgc cagctacgtc gttgcctgtt tctgtgtaca ttggaagtct
     5401 gggatcaatt ggatcgggaa caaatactcc cgtgaaatta catgccgtag ataatgagag
     5461 agccaataag aatgctttat ttttcatcgt aaatttcagt gattctcact tttatcccga
     5521 acaataaact acgatttttt acatcgtgaa tttcttcaat aaaatttcct aatttgtcaa
     5581 acatgtaata gttatagaca ggtttcaaac cgaggaaata atgaaaataa atacctgctc
     5641 ttctattttc aaataaattg atacctgaaa tccatgaaat atcgaattcg ctatacttag
     5701 cctcaaattg tgggccactt gcatcagcaa ggacttcata cgaaaagcca ttttgaatgg
     5761 ataaaaatct gaaggaatat tttgctagaa gcgtagaagt gatgtaagca agacttgcct
     5821 cgtgtttaaa tgatccgatt gtttgtgtgt atcctcttgt aatcaacgca gattgctttt
     5881 ccagagaaaa tttggatgac tttcgaaggg gtttaaaatt cattgcagca cctaaatgaa
     5941 tgccgggtgt gcctttaaca gaacctacaa gattttgttg ttttatcatg tttttgctaa
     6001 aaccaccaag catataaaat tgcgagagtg cattctgtgg gatcacaata aataaaaaga
     6061 accccaggaa aattttttga gtgcagcttg aaatcacgct ttagattgac attactttat
     6121 ataatatagg aaaaaaactg ttaaatatat aaatattcct attaaatccc aaatttctca
     6181 aatttgtgct gaatccgtca atcaaaatta aaaacccaaa accttatgtt gtccctagac
     6241 gcagtcggtg ttctttttac caaacggcca ttacgtaatc aactaactca gctcttgatg
     6301 tttttccacc aataagctaa acacagttag ataacttcat ttcattaacc atcaaaatga
     6361 accagaaaaa atctcaggaa ttttcagctg tcaaattaaa tctggcactg gagtacttat
     6421 acttccggat atttgcataa gccagctttg accggattat tgtgaatgta atcaattttt
     6481 tgccaaaata cttcctctct ccacaaggtc acagccaggg aattgcgctc ctaaacatga
     6541 tattttctgt ctttggcatt tacgtgtaat tgttcatgca aatcgaattt ctcaatcttt
     6601 aacactttac catcacaaaa aaagtaaatc tttatttggt ttatattata aactagttta
     6661 taattgagaa ttaatttaaa taatagaact atgaagcagg aaaataaccc gcttaccgcc
     6721 cagcaaagcc ttgatatcat caccaatatg atcagaca
//
LOCUS       DASWHX010000206        19052 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_2089026, whole genome shotgun sequence.
ACCESSION   DASWHX010000206 DASWHX010000000
VERSION     DASWHX010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 19052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19052
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2089026"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(155..616)
                     /locus_tag="VGA21_13620"
     CDS             complement(155..616)
                     /locus_tag="VGA21_13620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651596.1"
                     /translation="MVAFRQIQHAFILMFILALGILFLSSLFIYQAGTNPIPLFAFIL
                     ITILFAVVLLLFYRLKVVVFNDKILITFGVGLIWKIVDISRIKRIEIVRNPFYYGWGL
                     RIIPNGMLYNISGFKAVELHFHDTVRILRIGSANPAKLKQEIETRIRERIE"
     gene            complement(618..2018)
                     /gene="lpdA"
                     /locus_tag="VGA21_13625"
     CDS             complement(618..2018)
                     /gene="lpdA"
                     /locus_tag="VGA21_13625"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018472077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEX9651597.1"
                     /translation="MQYDVTVIGSGPGGYVAAIRCAQLGFKTAIIEKYNTLGGTCLNV
                     GCIPSKALLDSSEHYHNAEHNFKEHGIALKELSLDLKQMMKRKDEVVEQTTSGISFLM
                     KKNKIDVHTGIGSFVDKNTISIKDSKGKSTEIKSDKIIIATGSKPSSLPFINIDKKRI
                     ITSTEALTLNEVPKHLIVIGGGVIGMELGSVYARLGSKVSVVEFLDSLIPTMDKTMGK
                     ELQKIAKKLGMEVYVKHKVIAVETKGKNVTVTAETPKGDKIDIKSDYCLVSIGRRPYT
                     DGLNLEKVGIKTNKAGQIEVDEHLKTSADNIYAIGDVIRGAMLAHKAEEEGVLVAEQL
                     AGQKPHINYLLIPGVVYTWPEVASVGYTEEQLKEQGIKYKTGSFPFKASGRARASMDI
                     DGLIKVLADASTDEILGVHMIGPRAADMIAEAVVAMEYRASAEDIARMSHAHPTFTES
                     FREACLAATDNRALHI"
     gene            complement(2041..3591)
                     /gene="odhB"
                     /locus_tag="VGA21_13630"
     CDS             complement(2041..3591)
                     /gene="odhB"
                     /locus_tag="VGA21_13630"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009057371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HEX9651598.1"
                     /translation="MSLEIKVPTVGESITEVTIAQWLKKDGEIVEMDEVLCELESDKA
                     TFELNAEAAGKLTIKVQEGETVEIGAVICEIDENAKSDKKPAADKTEPAKASATPPKE
                     GKSSSGPVKTGKVKEMVVPTVGESITEVTISSWLKSDGDYVVLDEVIAEVDSDKATFE
                     LPAEASGFLSIVASEGTTLKIGDTICKIEVAEGAPAGKTDAPAQSPGKDVPVAETSKS
                     AGYYAESHPSPAAAKILAEKGIKPEEVKGSGVDGRITKADAEKATPKKQAPQKDTTPS
                     SAPKTAFSRNSTRKKMSNLRKTIARRLVAVKNETAMLTTFNEVDMKPVMSIRSKYKEQ
                     FKEKHGVGLGFMSFFTKACCVALQEWPAVNAQIDGYELVYNDFCDVSIAVSTPRGLVV
                     PVVRNAESMTFAEIEQEILNLATKARDSKLTIEEMTGGTFTITNGGVFGSMLSTPIIN
                     APQSAILGMHNIVERPMALNGEVVIRPIMYLALSYDHRIVDGRESVSFLVRVKQLLED
                     PSRLLLEV"
     gene            complement(3613..6327)
                     /locus_tag="VGA21_13635"
     CDS             complement(3613..6327)
                     /locus_tag="VGA21_13635"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017733290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HEX9651599.1"
                     /translation="MDKYSYIANAEIDYIDKLYKEYQQNPESVDITWQKFFEGFEFSI
                     QKFGENGHTDTEASPKEIQVSKLIHAYRARAHLRSNTNPVRKRKDRKAVLDLPDFGLS
                     EADLDLEFDSGEEIGIGRAPLRKIIESLKKIYEDTIGFEFMFIREPEILQWFMKKIEH
                     ERLNVKPGDLEIKRILGKLNEAVVFENFLHTKYMGQKRFSLEGGETTIPALDAIINKG
                     AELGVEEVVIGMAHRGRLNILANIMGKTYEQIFSEFEGGGDPDLTMGDGDVKYHKGFS
                     SQIKTPGGQVVNLKLAPNPSHLEAVNPVVEGFARAKADAEYDRQFDKIMPVLIHGDSA
                     VAGQGIVYEVVQMSKLDGYYTGGSIHFVINNQVGFTTDFDDARSSIYCTDVAKIIDSP
                     ILHVNGDDPEAVIFCVNVAVEFRQKFHRDIFIDMVCYRRHGHNESDEPKFTQPSLYNV
                     ISKHQNPREVYNKKLIERGDVGAELATQMDEEFRNLLQDRLNMVKQKPLPYSYQALEK
                     EWQNLRRSNPADFDRSPDTFISQKAIDKIANAITTVPDTFKPIKQIEKQLKQRNDMFF
                     KDKKLNWAAAELLAYGSLLLDGKIVRISGQDVQRGTFSHRHAVLRDVETNESYNSLNY
                     IEEGQGKFMIYNSLLSEFAVMGFEYGYAMANPNSLIIWEAQFGDFANGAQVMIDQFIA
                     PSESKWQRMVGLVLLLPHGYEGQGPEHSSARPERYLQLCAEYNMIVANVTEPSNFFHL
                     IRRQLAWPFRKPLIVMSPKSLLRHPAVESPISEFTKGHFREVLDDKYVTPTKVKRALL
                     CTGKIYYDLLEKQQNDKRKDVAIIRVEQLYPFPELQVDDILDTYKGPEVFWVQEEPAN
                     MGYWSYVHRIYRQNVKVLARKSSASPATGYAKVHVSEQQELVTSAFNV"
     gene            complement(6640..7092)
                     /locus_tag="VGA21_13640"
     CDS             complement(6640..7092)
                     /locus_tag="VGA21_13640"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX9651600.1"
                     /translation="MNEFLLVDSLDYLNKLLAVTYIIENTNNTLAFYSVFNDRVSVED
                     FDSNRKFGKWREMFGQRKKFKSYPAVKIGRLGVSNEYKGEGYGTSIIDYIKYLFINNN
                     RTGCRFVTVDAYKQSLKFYEKNGFQYFGSKDKDEDARQMYFDLLGLED"
     gene            complement(7185..7343)
                     /locus_tag="VGA21_13645"
     CDS             complement(7185..7343)
                     /locus_tag="VGA21_13645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651601.1"
                     /translation="MAKPIKETPFLEGKEAKRFLERMKNVQENKISQKEKERIRNNFA
                     KLDSIAAK"
     gene            7605..8159
                     /locus_tag="VGA21_13650"
                     /pseudo
     CDS             7605..8159
                     /locus_tag="VGA21_13650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015265175.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
     gene            8349..8867
                     /gene="rimM"
                     /locus_tag="VGA21_13655"
     CDS             8349..8867
                     /gene="rimM"
                     /locus_tag="VGA21_13655"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEX9651602.1"
                     /translation="MKKDNSILFGKIIKPHGIKGELRIDLLNPNFEYNKKMESVFVEI
                     DGKLVPFFIEDYSGRGPFGVLKLEGINTLEQAAGLHSKSLYINSKFIRSEQNSRPKIL
                     ELLSFQVEDIRLGNLGVVTDIEEGAQERLVVKKGGREILIPFTKPLIKTWDANSKIIL
                     VDLPAGFLDIYE"
     gene            8867..9550
                     /gene="trmD"
                     /locus_tag="VGA21_13660"
     CDS             8867..9550
                     /gene="trmD"
                     /locus_tag="VGA21_13660"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013453862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEX9651603.1"
                     /translation="MRFDIITCLPKLLESPFSDSIMKRAIDKKLVSVNIHDLRRFASG
                     KQKQTDDYAYGGGAGMVMMIEPIWNCISKLKAERNYDEIIYMTPDGESFNQKLANNLS
                     LKQNLILLCGHYKGVDERVREHLVTKEISIGDYVLSSGELAGSVLADAVIRLLPGVIS
                     DESSALTDSFQDNLLAPPVYTRPSDFQGWKVPDVLLSGHEKAIQDWRHEQSMERTRQR
                     RPNLLDGDS"
     gene            complement(9547..10299)
                     /locus_tag="VGA21_13665"
     CDS             complement(9547..10299)
                     /locus_tag="VGA21_13665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018341931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9651604.1"
                     /translation="MSNTNIRVIIIDDEEPARVIIRSFLEKYAGFEVISECENGFEGI
                     KMINEQNPDLIFLDIKMPKITGFEMLELLDKIPAVIFSTAYDEFAIKAFEMNAADYLL
                     KPYSKERFHHAIQKAKEKLGHEQSQKLQPLIDYAKDQETELNRIVVRYGSKIHIFPTS
                     ELHYIEAQDDYVKFFFQKGSFLKQGTMKYYEEHLPSNDFVRIHRSYILNLNQLLRLEP
                     FEKESFVVVLKVGGNLPVSRSGYSRLKEVLNF"
     gene            complement(10292..11341)
                     /locus_tag="VGA21_13670"
     CDS             complement(10292..11341)
                     /locus_tag="VGA21_13670"
                     /inference="COORDINATES: protein motif:HMM:NF025854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX9651605.1"
                     /translation="MMSHPLYQKPANRFYLIIWSVISIGHFCIFYFFYNFSILASALD
                     SLIFNTLYFGLGLGIWYLVRFNPLNKRLSRTILNHAISGVIAVILWASIGKSLIGILP
                     IEDKAYENFLANSNLGRNLIGFALYALFSLSFYLIEHYESLQDRIREELELKALVNEA
                     HLKSLKSQINPHFLFNSLNSINSLTLVSPEKTRDMVIKLSEFLRYSAGKQSQEHATLE
                     EELRHMNIYLDIEKVRFGKKLDIETSIDETCLKAKIPNFILQPLLENAIKYGVQESLD
                     TKPITIKCSKSGTFLRIEVENQFDDTINAKGEGIGLNNIKARLKILYGRDGLIQMESA
                     NNLFKVIIEIPQENE"
     gene            complement(11370..12062)
                     /locus_tag="VGA21_13675"
     CDS             complement(11370..12062)
                     /locus_tag="VGA21_13675"
                     /inference="COORDINATES: protein motif:HMM:NF021443.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HEX9651606.1"
                     /translation="MSNEANSRRAWFGIGILVVGATFLARNLGFIPHEIEIYMGWELI
                     FILIGTVMMVSGKSGGLVFLLIGGFFLLQDITHFYFRLRDWWPVILIIIGVFMLLRQA
                     GRGKGDGGSGIAKGNRDTDTIDDVNIFGGGSKNITSKNFKGGKVTSIFGGADLNFHDA
                     IPAPENAVIDLFTMFGGCTLYVPSDWTVRMEEFSLFGGFSDNRRNVKADSNKVLIVKG
                     LVIFGGGEIKTA"
     gene            complement(12192..12323)
                     /locus_tag="VGA21_13680"
     CDS             complement(12192..12323)
                     /locus_tag="VGA21_13680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651607.1"
                     /translation="MKNKNTSFLQSIHNANRSLILAIKEVLLSSKEAEKFNTILQIG"
     gene            12464..12814
                     /gene="rplS"
                     /locus_tag="VGA21_13685"
     CDS             12464..12814
                     /gene="rplS"
                     /locus_tag="VGA21_13685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017733285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEX9651608.1"
                     /translation="MSELLKLVEQQFKLGTEIPAFKAGDTINVHVKIKEGNKERVQQF
                     LGTVIQRKNKGTLGETFTVRKVSNGVGVERIFPISSPSIEKIEVTRKGSVRRARLFYL
                     RGRQGKAAKIKEKQ"
     gene            12902..13960
                     /locus_tag="VGA21_13690"
     CDS             12902..13960
                     /locus_tag="VGA21_13690"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007657144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HEX9651609.1"
                     /translation="MSYQKIVKLLGDKSDSLLKHESKTIGKDQLHPMGPDYVDRILSP
                     SNRNPQVLRNFQSILNSGRLAGTGFVSILPVDQGIEHTAGASFAPNPIYFDPEKIVEL
                     AVEGGCNAVATTFGNLGLVSRKYAHKIPFLVKLNHNELMTYPNKFDQIMFGSVEEAWN
                     LGAAAVGATIYFGSEESNRQIVEVAEAFERAHQLGMVTVLWCYARNNEFKKDGVDYHV
                     AADLTGQANHLGVTIQADIIKQKLAENNGGFTAIKFGKSHPAMYDKLASGHPIDLCRY
                     QVANCYMGRIGLINSGGASSGASDLAEAVMTAVVNKRAGGMGLISGRKAFQRPMKEGV
                     ELLNTIQDVYLAAEIGLA"
     gene            13983..14825
                     /gene="accD"
                     /locus_tag="VGA21_13695"
     CDS             13983..14825
                     /gene="accD"
                     /locus_tag="VGA21_13695"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010609802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HEX9651610.1"
                     /translation="MSWFQRKDKGILTPTEKKKEAPDGLWFKTPNGKIVHIRELKSNA
                     YVSPEDGYHVRIGSREYFEILFDDNKFTELDKDMISADPLGFSDGKPYAERIKQGQVK
                     SGYKDAVRTAFGKVNGIDWTVACMDFTFIGGSMGSVVGEKIARAIDHSIKNKIPLLII
                     SKSGGARMQEAGLSLMQMAKTSAKLALLSEAKVPFVSLLTDPTTGGVTASYAMLGDFN
                     IAEPGALIGFAGPRVIRETIGKDLPKGFQSSEFLLDRGFIDFIIDRRNLKSRLTSLVG
                     MLRN"
     gene            14867..15847
                     /locus_tag="VGA21_13700"
     CDS             14867..15847
                     /locus_tag="VGA21_13700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015330016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX9651611.1"
                     /translation="MRLIAKPVLCNYYVTYRCNATCGFCDIWEKPSPYIDLDQARKNL
                     FDLRKLGVKVIDFTGGEPLLYRELSTLLKEAKDLGFVTTITTNCLLYPKRASELRGLV
                     DMLHFSLDSGVKQMHDEMRGVACFDKVMESIEIAKSLEERPDILFTAFDHNLDQIEKI
                     YYEIVLPNNLVLILNPSFSYNRVEASLDERSLNILSSWSNKKNIYLNDAFIELRRDGG
                     NHTANPVCKAASSTIVISPENKLILPCYHLGIQEFKINGDLFTLYHSSEVQNLVALEG
                     RLPECEGCTINCYMQPSFAVELNKYWWKALPSTLKYNAIKGTWKMALKAI"
     gene            15944..17245
                     /gene="nqrF"
                     /locus_tag="VGA21_13705"
     CDS             15944..17245
                     /gene="nqrF"
                     /locus_tag="VGA21_13705"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002696849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit F"
                     /protein_id="HEX9651612.1"
                     /translation="MLPVLTSIAAFTAVIVLLVLVLLYAQSKLVQSGDVDIIINGDAS
                     NPLTVSAGSTLLTTLSAQKIFLPSACGGGGTCAMCKCQVLEGAGDVLPTEVGHLSRKE
                     QQDHVRLACQVKVKQDLKIKIPEEIFGIKKWECEVVSNYNVSTFIKEFVVKLPEGEIL
                     NFQSGGYIQIDVPETVVHFKEMDITPKPELNHKPDVFQNDWDKFQLWGLTMKNSEPIF
                     RAYSMANHPAEGNIIMLNIRIATPPWDRANNKWMDVNPGICSSYVFSRKLGDKVTISG
                     PYGEFHINPTQREMIYIGGGAGMAPLRSHIFHLFHTEKSNRKVSYWYGGRSKKELFYT
                     NHFRSIEKDFPNFSFHIALSEPLQDDNWSIKKSLDDKDSDGYIGFIHQVLYDNYLKLH
                     KEPEEVEYYLCGPPLMNAAVLQMLDEMGIPSENIRFDDFGG"
     gene            17304..18464
                     /locus_tag="VGA21_13710"
     CDS             17304..18464
                     /locus_tag="VGA21_13710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009579238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamase"
                     /protein_id="HEX9651613.1"
                     /translation="MDLKLFFSPAGKETSSGIKSKTALLKYLHVFQDEMPEIKEANIA
                     LIGITESRGSAGNDGVIRAPDNIRKVLYNLKKGFGNYRIVDLGNLRNGMSFEETNGRV
                     REVCQFLIQNNVLPVLFGGTHAIDYGQYQAYDGLEKLVTVLNVDAFLDMEDPDGKEPD
                     LDHTNRIFLHEPNYLFHYSHLAYQSYLVNRDTLSVLEKLYFETHRLGNLHTNLVEMEP
                     VIRNADMLTFDISAIKSNDAPGSSQAQPFGLTGEEACQICWYAGLNEKLSSAGFYQYN
                     PDKDDADNKTAKVVATMIWYFIEGYYHRQNEQNFKANDYMKYVVSMPADPETLIFYKS
                     KASEKWWMEVPIPKSGGKYKRDYIIPCTYSDYEKSTSGDVPDRYISTLAKLV"
     gene            complement(18573..>19052)
                     /locus_tag="VGA21_13715"
     CDS             complement(18573..>19052)
                     /locus_tag="VGA21_13715"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEX9651614.1"
                     /translation="SDFDPSTLNSGASGDSDLYADRKIDMLANGNFSADETVRIRFRL
                     WSDELAVGWGWVIDNLRIQSLITGIDEVIDEESFVIYPNPSSGNFTLKAQLNEPISNS
                     RIHVTNLLGKSIFLKQLDSRNTIVNEVIDLGNFPDGLYIITLETPKGRITRKVIKVY"
BASE COUNT         5581 a         3920 c         3905 g         5646 t
ORIGIN      
        1 attacgatcg gtttatcagg tccaaaagat gaaaacggcg acagcaaacc ggtaaccatt
       61 tccgatgacg ctgggattaa ttaaacggtg gatgctggga ggtgaagaga atctcaaaat
      121 tattaacaag gaattagaga atcagatgaa tgttttattc tatacgttcc ctgattctcg
      181 tttcaatctc ctgctttaac ttcgcggggt tggcgctgcc gattcttaaa attctgacgg
      241 tatcatgaaa atgcaattcc accgctttaa aaccggaaat attgtaaagc attccattgg
      301 gaataattct caacccccag ccatagtaaa atggatttct cacaatttca attctcttaa
      361 tcctggaaat atccacgatt ttccaaatta aaccgacgcc aaaagtaatc agaattttgt
      421 cattgaatac caccactttc aaccggtaaa aaagaagcag caccacagca aagagaatgg
      481 tgatgagaat aaatgcaaat aaagggatgg gattggttcc tgcctgataa atgaacagag
      541 acgacagaaa tagaattcct agcgccagaa tgaacatcag aataaaagca tgttgaattt
      601 gtctgaaagc taccatacta tatatgcaaa gccctgttat cagtagcagc cagacatgct
      661 tccctgaaag attctgtaaa ggtaggatgt gcatgactca ttcgtgcaat atcttcagca
      721 gatgctctgt attccattgc taccactgcc tcggctatca tgtcggcggc gcgcggccct
      781 atcatatgta ctcccaaaat ttcatccgtt gaggcatcag ccagaacttt gatcagtcca
      841 tcaatatcca tactggcgcg agctcgtcct gaagctttga aagggaatga accggtttta
      901 tattttattc cctgttcctt taattgttcc tccgtataac ccacgctggc aacttcgggc
      961 caggtataaa ctactccggg aataagcagg taatttatgt gcggtttttg accggcaagt
     1021 tgctcggcaa ccagcacacc ttcttcttcc gctttatgag caagcattgc cccgcgaatt
     1081 acatcaccaa tcgcgtaaat attatccgct gaagttttaa gatgttcgtc aacttctatc
     1141 tgacctgctt tattggtttt tattcccacc ttttcaaggt taagtccatc agtatatggg
     1201 cgtcgcccga ttgacaccag gcaatagtca cttttaatat caatcttgtc accctttgga
     1261 gtttcggccg tcaccgttac gttcttgccc ttggtttcga cggctattac cttatgcttt
     1321 acgtatactt ccatgcccag cttttttgca attttttgca gttctttacc cattgtctta
     1381 tccatagtag gtatcagact atcgagaaac tctaccacag aaactttgga acccaaccgt
     1441 gcatacacag aacccaattc cattccaata actccgcccc caattacaat caaatgtttt
     1501 ggtacttcgt tgagagttaa agcttcggtg ctggtaataa ttcttttctt atctatgttg
     1561 ataaaaggaa gcgatgatgg ttttgatccg gttgcaataa taattttatc agatttgatt
     1621 tcggttgatt ttcccttgct gtctttgatg cttattgtat tcttatcaac gaatgaaccg
     1681 attccggtgt gaacatcaat cttattcttc ttcatcagaa aggaaattcc acttgtagtt
     1741 tgttccacta cctcgtcttt ccgcttcatc atttgcttca ggtcgagcga cagttctttt
     1801 aacgcgattc catgctcttt aaaattgtgt tcggcgttat gataatgttc tgaggaatca
     1861 agtagcgctt ttgaaggaat acaccctaca ttaaggcagg tgccgccaag cgtattatat
     1921 ttttcaataa ttgcggtttt aaaccctaat tgtgcgcacc ggatggcagc gacatagccg
     1981 cctgggcccg agcctattac agtaacatcg tattgcatgg gtattttttt tcaggttgaa
     2041 ttaaacttct aataataatc ttgatgggtc ttccaatagt tgcttaaccc ttacaaggaa
     2101 actcacagat tccctgccgt caacgattct atggtcgtaa gacaacgcca gatacataat
     2161 aggccttatt accacttctc cattaagcgc catggggcgt tcaacaatat tatgcattcc
     2221 cagaatagcg gattgtggtg cattaattat gggtgtggaa agcattgaac cgaaaactcc
     2281 accattggtg atggtaaaag tacctccggt catttcttcg atagtaagtt tgctatccct
     2341 ggctttggtg gcgaggttca gaatttcctg ttcaatttca gcgaaagtca ttgattcagc
     2401 atttcttact acaggtacca ccagcccacg gggagtagaa accgcaatag aaacatcaca
     2461 aaaatcattg taaaccaatt cgtatccatc aatttgagca ttaaccgcag gccattcctg
     2521 caatgcgacg caacaagctt tggtaaaaaa tgacatgaag ccaagtccta caccatgttt
     2581 ctccttgaac tgttctttat attttgaacg tattgacatc acgggtttca tatccacctc
     2641 attaaaggtg gtaagcattg cagtttcatt cttgacggca actaacctgc gagcaatggt
     2701 ttttcgcagg ttcgacattt tcttacgcgt ggaatttcgc gagaacgcgg tctttggtgc
     2761 tgatgaaggt gttgtatctt tttgtggagc ttgcttcttc ggcgttgctt tttccgcatc
     2821 tgccttggta atcctgccat caactccact tcctttaact tcttcgggtt taataccttt
     2881 ttcagcaaga attttcgccg cagccggtga tggatgactt tccgcatagt accccgcgct
     2941 tttagatgtt tcagcaaccg gtacatcctt tccaggggat tgtgccgggg cgtcagtttt
     3001 accggcaggt gcgccttcgg caacttcaat tttgcaaata gtatctccaa ttttcagggt
     3061 tgttccttcg ctggcaacga tgctaagaaa tcctgatgct tctgcaggca attcaaaagt
     3121 agccttgtca gaatcaacct ccgcaattac ctcgtccagc acaacataat cgccatccga
     3181 ttttagccag gaactaatgg taacttccgt aattgattca ccgactgtcg ggactaccat
     3241 ttcttttact ttcccggtct taaccggacc ggaggaagat ttaccttctt tcggaggagt
     3301 tgcacttgct ttggccggct ctgttttatc agctgccggt tttttatctg actttgcatt
     3361 ttcatctatc tcgcagataa ctgctccaat ttctacggtt tcaccttcct gcactttgat
     3421 agtaagttta ccggcggctt ccgcgttaag ctcaaaggtt gccttatcgg attcaagttc
     3481 acaaagaact tcatccattt ccacaatttc tccgtctttt ttaagccact gggcaatcgt
     3541 tacttccgta atagattctc ctacggttgg tacttttatt tcaagactca taagtaattt
     3601 ctttttatgg gtttaaacgt tgaaggctga ggtaactaac tcctgttgtt cggatacatg
     3661 cactttggca taacctgttg ccggcgaagc actgcttttt ctggccaaca cttttacatt
     3721 ttgccggtaa atgcggtgga cgtacgacca ataccccatg ttggctggtt cttcctgcac
     3781 ccagaagact tcaggccctt tgtaagtatc cagaatatca tctacctgca attcaggaaa
     3841 aggataaagt tgttcaaccc ggataatagc cacatctttc cgcttatcat tttgctgctt
     3901 ttcgagaagg tcataataga ttttacctgt gcataaaaga gctcgtttca ctttggttgg
     3961 tgttacatat ttatcatcaa gtacttcccg gaaatgacct tttgtaaact cagagattgg
     4021 cgattctacc gcagggtgcc tgagaaggga tttgggtgac attactatca gaggtttcct
     4081 gaatggccag gctaattggc gacgtataag gtgaaaaaaa ttagagggtt ctgtaacatt
     4141 agccacaatc atgttgtatt ctgcacaaag ctgcagatat cgctccggtc tggcactgga
     4201 gtgttcgggt ccctggccct cgtatccatg tgggagtaaa agtaccaaac ccaccattcg
     4261 ctgccattta gattctgacg gtgcaatgaa ttgatcaatc atgacctggg ctccatttgc
     4321 aaaatctcca aactgcgctt cccatataat taaggaattc ggattagcca tcgcgtaacc
     4381 atattcgaaa cccatgaccg caaactcgga aagcagggaa ttataaatca tgaattttcc
     4441 ctgaccttct tctatataat tcaaactatt atatgattca ttggtttcaa catcacgaag
     4501 tacagcgtgc cggtgtgaaa acgttccccg ttgaacatct tgcccggaaa ttctcacaat
     4561 ttttccatca agcagaagag aaccgtaagc taataattcc gccgccgccc agttcagttt
     4621 tttatctttg aaaaacatgt cgttccgctg cttaagctgc ttttcaattt gtttgattgg
     4681 cttaaacgta tccggaactg ttgtaattgc attcgctatt ttatcaatgg ctttttgtga
     4741 aataaaagtg tcgggtgacc ggtcaaagtc ggcaggattt gatcttctca gattctgcca
     4801 ctccttctcc aatgcctggt aggaataggg aaggggtttt tgtttaacca tatttaacct
     4861 gtcctgaaga aggttcctga attcttcgtc catttgggta gcaagctcag cacccacatc
     4921 accgcgttcg atcagtttct tgttatatac ttctcttggg ttttgatgtt tcgaaattac
     4981 attgtaaagg gagggttggg taaattttgg ttcatcactt tcgttatggc cgtgacgccg
     5041 gtaacacacc atatcaatga aaatgtcacg atgaaatttc tgccggaatt caacagcaac
     5101 atttacgcaa aatattactg cttccggatc atcaccatta acatgcaata tcggactgtc
     5161 gataattttt gctacatctg tacaataaat agaggatcgg gcatcatcaa agtcggtagt
     5221 aaatccaacc tggttgttta ttacaaagtg aatactaccc ccggtgtaat aaccatcaag
     5281 tttagacatt tgcacaactt catagacgat tccttgtcct gccaccgcag aatcaccgtg
     5341 gataagcacc ggcattattt tatcaaattg ccggtcatat tctgcatcag cttttgcgcg
     5401 ggcaaatcct tccaccacag gatttactgc ttccaaatgc gagggattcg gagccagctt
     5461 cagattcact acctgaccac cgggagtttt aatctggctg gaaaatcctt tgtggtactt
     5521 tacatcaccg tcacccatgg ttaaatccgg atcaccgcca ccctcaaatt cactgaatat
     5581 ttgctcatag gttttgccca taatatttgc caggatattt agccggcccc ggtgtgccat
     5641 ccctatcact acttcttcaa cgcccaactc tgcacccttg ttaataatag catccaaagc
     5701 aggaattgta gtttcacccc cttccagcga aaaccttttc tgacccatgt atttggtgtg
     5761 aaggaagttt tcaaagacca ctgcttcatt gagtttgcca agtatccttt ttatctccag
     5821 atcgccaggc ttaacattga gtcgttcgtg ttcaattttc ttcataaacc attgcaaaat
     5881 ttccggttcg cggatgaaca taaactcaaa gccaatggtg tcctcgtaaa tctttttaag
     5941 agattcaata atctttctca acggtgctct tcctattccg atttcctcac cgctgtcaaa
     6001 ctccaaatca aggtcagctt cactcaatcc aaaatcaggg agatccagca ctgctttgcg
     6061 gtcttttcgt tttctgaccg gattagtatt tgagcggaga tgagctcgtg ccctataagc
     6121 atgaatgagt ttgcttacct ggatttcttt aggtgaagct tctgtgtccg tatgtccatt
     6181 ctcgccgaat ttctgaatgg aaaactcaaa gccttcgaaa aatttctgcc aggtaatgtc
     6241 aacagattca gggttttgct ggtattcttt gtaaagtttg tctatgtagt caatttcggc
     6301 gttggcgata taggagtact tatccataac ggggtttatt tacagcggga aacaaatgta
     6361 tgggtttagg agttattatt aaaactttat atgcggttat acgaatatag ctgcttagcc
     6421 aggttacaaa tgttctggaa ttagggtcaa attacaggtc tgtttcccat tttgaaaaga
     6481 aattcaggtg tgttgaaagg gctagaaaag ctggttatgt gggcgggtaa aaataaagcc
     6541 gaaaagctga gcaagaaaga cggtcagaga tagctaagcg cagctaataa aggtggagta
     6601 aagattcctc tttagaatct ttaatatttc cctaagggtc taatcctcca gccctaacaa
     6661 atcaaaatac atttgtcgag catcttcatc cttatctttt gatccaaaat actgaaatcc
     6721 atttttttcg tagaatttta acgattgctt gtacgcatca accgtaacaa acctacatcc
     6781 tgttctatta ttattgataa acaaatactt aatgtagtct attattgaag tgccataccc
     6841 ttctccctta tattcattgg aaactcctag ccttcctatc tttacagcgg gatagctctt
     6901 gaatttcttt cgttgtccaa acatttccct ccattttcca aattttctat tgctatcgaa
     6961 atcctctaca gatacccggt cgttgaaaac agaataaaat gcaagcgtat tattagtgtt
     7021 ttcgataatg taagtaacag cgagcagctt gttaagataa tctaatgaat ctactagtaa
     7081 gaattcattc aaatcagaat ctccacagtc aaagggcttt atagtatgtg tatcatgaag
     7141 ccttataagc ctagcatcat caggcaggtc cgttcgacta tttgctattt ggcagcaatc
     7201 gaatctagct tggcaaagtt gtttcttatt ctctccttct ctttctgact aatcttattc
     7261 tcttggacgt tcttcatcct ctcaagaaac ctctttgctt ctttaccctc aagaaatggt
     7321 gtttctttta taggcttagc catttttatt aatgaattta tagactttat gaatttcgcc
     7381 ggattggatc agactttgtc aacaccaaga gacaggcaac aaatgtatta caaagataat
     7441 accttaatat tctcattaca actactacta atgcttgcac cttgtacctt acttttagtt
     7501 ctaccctctc caaattattt agttcagatg ctgtttccac ggaatttgcc ttatctttgc
     7561 ccctctttta aaatgaggac gtgatcctta aaaaaaatta aaaaatggtt aaaatgagat
     7621 tagcccgcag agggcgcaaa aatctggcac tgtacgatgt agtcgtagcc gacgccagag
     7681 ctccacgaga tggtcggttt attgaaaaaa tcgggactta taaccccaac accaaccctg
     7741 cactggttga actggatgaa gagaaagcct tcaaatggat aatgaacggc gcccagccta
     7801 ctgataccgt cagggcaatg ctctcataca ggggagtttt agtgcgaaag catttgcagg
     7861 tgggtgtaat taaaggcgct ttgacacagg aacaggctga cgagaagttt gaagcctgga
     7921 aaaaggaaaa agccgcaaaa atccagacta aagtagacag gctggctgcg gaaaaggaaa
     7981 agcaggataa agaaaaactg gcggctgaat ctaaggtgaa agaagccaga gctgaagcct
     8041 taaagaaaag gcagcaggta gccgagcagg aagccaaaac tgctgaagct gccgaagaag
     8101 taacggctga atcgccggca gaagttcagg aaaccgcgga agcaaccccg gctcaaacag
     8161 aaacgactgg agaatctcct gttgaggctc ctgaagagtc taaaactgag gagacttcaa
     8221 aagaggcgga aataaaaact gaagcggaac ctatagctga aacaccggca gccgtagaac
     8281 caaagtctga ggagcaaaaa aaggcagact cggaagcaga agaaaagaag gctgaataat
     8341 gttactgaat gaagaaggat aattcaatcc tcttcggcaa aattattaag ccacacggga
     8401 taaagggtga actcagaatt gacctgctaa atcccaattt tgaatataat aaaaagatgg
     8461 aatcagtatt tgtcgaaatt gatggcaaat tggttccatt ttttattgaa gattactcag
     8521 gcagaggtcc ttttggagtg ttgaagttag aggggataaa cactctggaa caagcggccg
     8581 gccttcattc caaatcactt tacattaata gtaaattcat cagatctgaa caaaattccc
     8641 ggcctaaaat cctggaatta ctttcatttc aggttgaaga tattcggtta ggcaatcttg
     8701 gggtggtaac agacattgaa gaaggtgcac aggaaaggct cgttgtcaaa aaaggcggac
     8761 gggagattct gattccgttt acaaaaccgc tgattaaaac atgggatgca aattcaaaaa
     8821 tcattctggt tgatcttccg gcaggatttc tggacattta cgaataatgc ggtttgatat
     8881 aattacatgc cttccaaagc tgctggaaag tccattttca gattccatca tgaaaagagc
     8941 tattgataag aaactggtga gtgtcaacat tcatgatttg cgccggtttg cttcgggtaa
     9001 acaaaagcaa actgatgatt atgcttatgg agggggagcc ggcatggtaa tgatgattga
     9061 acctatctgg aattgtattt caaaactgaa agccgagcgt aattatgatg aaatcattta
     9121 tatgactccg gatggagaat catttaatca aaagttagcc aacaaccttt ctttaaagca
     9181 aaatcttatt cttctatgcg gtcattataa gggagttgat gaaagggtaa gggagcatct
     9241 ggttacaaag gaaataagca ttggcgacta cgtactttcc tccggtgagc tggcaggttc
     9301 ggtcttggca gatgctgtga tcaggttgct tccgggtgtt atttctgatg aatcctccgc
     9361 acttactgat tccttccagg ataatttact ggcgccgccc gtttatacca gaccctcaga
     9421 tttccagggc tggaaagttc ctgatgtatt attatcaggt catgaaaaag ctattcagga
     9481 ttggcgtcat gaacaatcaa tggagagaac aaggcagcga agaccaaatt tgctggatgg
     9541 agattcctaa aaattcaaaa cctctttcag cctcgaatag ccggagcgac taaccggtaa
     9601 attaccccct acttttaata ccactacaaa ggattccttt tcaaaaggct ccagtcgcag
     9661 caattgattt aagttcaaaa tatacgaacg atgtattctt acaaagtcat tagaaggtaa
     9721 atgctcttcg tagtatttca ttgtaccctg tttcagaaaa gatccctttt ggaaaaagaa
     9781 tttgacataa tcatcctgag cttcaatata gtgaagttcc gaggtaggaa atatatgtat
     9841 cttagatcca tatcgcacaa caattctgtt tagctctgtt tcctgatctt ttgcataatc
     9901 aattaatggt tgcagctttt gggattgctc atgtcccagc ttttcctttg ctttttgaat
     9961 ggcatgatga aacctttcct tggaataagg cttcagcaaa taatcggccg cattcatttc
    10021 aaatgctttg atagcaaatt catcatacgc cgtactgaaa ataactgccg gaattttatc
    10081 caataattcc agcatttcaa agccggtaat tttaggcatt ttgatatcaa ggaaaatcaa
    10141 atcaggattt tgttcattaa tcatcttaat tccttcaaaa ccattttcac attctgagat
    10201 aacttcaaag cccgcgtact tttccaaaaa tgatctaatg ataactcgcg ccggttcttc
    10261 gtcgtcaatt attatgactc taatgttagt gttactcatt ctcctggggt atttctatga
    10321 ttactttaaa aaggttattt gctgattcca tttgtattaa tccatcacga ccataaagaa
    10381 tctttagcct ggccttaatg ttgttaagtc cgattccctc cccttttgca ttaattgtat
    10441 catcaaattg gttttccact tcaattctaa ggaaagtgcc ggattttgaa cacttaatgg
    10501 taattggctt tgtatccagg ctttcctgga cgccatattt gatcgcattt tcaagcaatg
    10561 gctgcaaaat aaagttagga attttggctt taaggcaggt ttcgtcaatg cttgtttcta
    10621 tatccagctt cttgccaaac cgtactttct caatatccag gtagatattc atgtgccgca
    10681 gttcttcttc cagggttgca tgctcctggc tttgtttacc agccgaatac cggagaaatt
    10741 cagataactt aatgaccatg tccctggttt tttccggtga aaccagggtc aatgaattta
    10801 ttgaattcaa gctattgaaa aggaagtgcg gattaatttg gctttttaag gattttagat
    10861 gggcttcatt cactaatgcc tttaattcca actcttctct gattcggtcc tgcaaacttt
    10921 cataatgttc gataaggtaa aagctaagag aaaacaaagc gtagagagca aatccaatca
    10981 ggtttcttcc aagattcgaa ttagcaagaa aattttcata ggctttatcc tcaatgggta
    11041 gaattcctat caatgatttg ccaatgcttg cccaaagaat taccgcaatt acccccgaga
    11101 tcgcgtgatt gagaattgtt ctggaaagtc ttttatttaa gggattgaaa cgaaccagat
    11161 accagatacc cagacccagc ccaaaatata aagtattgaa gataaggcta tccaacgcgg
    11221 aggcgagaat tgaaaaatta tagaagaaat agaaaatgca gaaatggccg atggagatca
    11281 ctgaccagat aatcaaatag aatctattcg cgggtttttg atagagggga tgactcatca
    11341 ttcaatagta tgcagatatt aatccctgac tatgccgttt taatttctcc gccgccaaaa
    11401 attaccaaac cttttacaat caacacttta ttggaatcgg ctttaacatt tctacggtta
    11461 tccgaaaaac ctccaaataa gctgaactct tccattcgca cagtccagtc ggagggtacg
    11521 tataaagtgc atcctccaaa catggtgaaa agatcaatta cagcattttc gggggctggt
    11581 attgcatcat gaaaattcaa atccgcacct ccgaagatgg atgttacttt acctcctttg
    11641 aaattttttg aagtaatatt tttgctgcca cctccgaaaa tgttcacatc atcgatggta
    11701 tcggtatccc tgttgccttt ggcaataccg ctaccaccat cacccttgcc tcgtcctgcc
    11761 tgacgtaata acatgaaaac accaattatg ataaggataa ccggccacca atctcttaat
    11821 ctgaagtaaa agtgagtgat atcctgtaac aagaaaaaac caccgatcaa aaggaatacc
    11881 aatcctccac tcttgccgga taccatcatg acagttccga taagaataaa tatgagttcc
    11941 caacccatgt aaatctcaat ttcgtgagga ataaaaccga gatttctggc caggaaagtt
    12001 gccccaacca caagaatccc tatcccgaac catgcccggc ggctatttgc ttcatttgac
    12061 atttctttag tgtttaaaat ccattaaaat taacgcagaa tcagtattta caaaggtcct
    12121 atttcggtga ataaacaata aatccgggtt aaccactgtt cagacggggt aggagctctc
    12181 acgagcagct gttatcctat ctggagaata gtattaaatt tttcagcctc cttcgaactt
    12241 aacaaaacct ctttgatagc aagaattaat gaacgatttg cgttatggat gctctgaagg
    12301 aatgatgtat tcttattttt cattttaaaa accccctaat tttaacaaaa tagcctatta
    12361 ctcaattcat taaaatttct ttgaaaacac aatgatcaat aaattaaaag atgattgaga
    12421 attcttttct atatttgcag tcctaaaaat taagcatcag gtcatgagcg agttactaaa
    12481 gctggtggaa caacaattca agttaggaac tgaaattccg gcatttaagg caggtgacac
    12541 aataaatgtg cacgtgaaaa ttaaagaagg aaataaagaa agggttcagc aatttctggg
    12601 gacagtaatc cagagaaaga ataaaggaac attgggagaa actttcacgg ttagaaaagt
    12661 atcaaacggt gttggagttg agcgaatatt tcctatttct tcaccttcaa ttgagaaaat
    12721 tgaagttacc cgaaaaggat cagtgcggag agcgaggctg ttttatttaa gaggtcgcca
    12781 gggcaaagcc gcgaaaatca aagaaaagca ataattatct caaatcgttc atattaagcc
    12841 agccgtttac ggttggcttt tatatttaaa tttgttataa taatccaaat ccaatcacga
    12901 tatgtcttat cagaaaattg taaaacttct tggcgataaa tcggattcat tgctcaagca
    12961 tgaaagcaaa acgataggga aagatcagtt gcatcctatg ggtcctgatt atgttgacag
    13021 aattctgtcc ccttcaaaca gaaacccgca agttctaaga aattttcagt caatattgaa
    13081 tagcggaaga cttgcaggta ccggatttgt atctatttta cctgttgacc agggaattga
    13141 gcacaccgcc ggggcctcat tcgcaccaaa tccaatttat tttgatccgg agaaaattgt
    13201 ggaattggcc gttgaaggtg gatgcaatgc tgttgccacg acctttggaa atcttggtct
    13261 tgtaagccga aaatacgcgc ataaaatacc tttcctggta aagctgaatc ataatgaatt
    13321 gatgacttat ccaaacaagt ttgatcaaat catgtttggt tcagtggaag aagcctggaa
    13381 cctgggagcc gcggctgtag gagcgaccat ttattttgga agtgaagaat caaaccgcca
    13441 gattgtagaa gttgccgaag cttttgaaag agcacatcag ttgggaatgg tcaccgtcct
    13501 ttggtgctat gcccgcaata atgaatttaa gaaagatgga gtagattatc atgtggctgc
    13561 cgacctgacg ggtcaggcaa atcaccttgg tgtaactatt caggccgata ttatcaagca
    13621 aaaattagct gaaaataacg gcggattcac agccataaaa tttggcaaat ctcatccggc
    13681 aatgtatgat aaattggctt ccgggcatcc tattgatttg tgcagatatc aggttgctaa
    13741 ttgttatatg ggtcgtattg gtcttatcaa ttcgggagga gcttcttccg gtgcttccga
    13801 tttagcagag gcggttatga ctgccgtagt gaataaaaga gcaggcggaa tgggtcttat
    13861 ttcaggcagg aaggctttcc aaaggcccat gaaggaggga gttgaactcc tgaataccat
    13921 ccaggatgtt tacctggcgg ccgagattgg tttggcctag caatgatcta acttagagtt
    13981 atatgtcctg gttccaaagg aaagacaaag ggattttaac cccaactgag aagaagaaag
    14041 aggcgcctga tggtctttgg ttcaagaccc cgaacggaaa gattgttcat attagagaac
    14101 tgaaaagtaa cgcttatgtt tcccctgaag atggctacca cgtaaggatc ggctcgcgcg
    14161 agtattttga aatccttttt gatgataaca agttcacaga actggacaag gatatgattt
    14221 cagccgatcc tctgggtttt tcagacggga agccgtacgc tgagcgaatc aagcagggac
    14281 aggttaaatc agggtacaaa gatgctgtgc gtaccgcttt cggtaaagta aacggcatag
    14341 actggacagt agcgtgcatg gactttactt ttattggagg ttcgatgggt tctgtggtag
    14401 gagagaaaat tgcccgcgct attgatcatt caatcaaaaa caaaattccc cttttaatca
    14461 tttccaagtc ggggggagct cggatgcagg aagccggttt gtctttgatg caaatggcaa
    14521 aaacttccgc aaaactggca ctcttaagcg aggccaaagt tccgtttgtt tccctgctca
    14581 ccgatcccac caccggcggt gtcacagctt catacgcgat gctgggagat ttcaatattg
    14641 ccgaacctgg tgctttgatt ggattcgccg gtccacgggt tattagagaa acaataggaa
    14701 aagatcttcc aaaaggattt cagagcagcg aatttctact ggatagggga tttattgatt
    14761 ttattatcga caggagaaac ctcaaatcca ggttaacttc actggtagga atgctccgga
    14821 attaatctcc attttttctt tcctccggat ttcggtaatt ttctgaatgc gattgatcgc
    14881 gaaaccggta ctatgcaatt attacgtcac ttacagatgc aatgccacct gtgggttttg
    14941 cgatatctgg gagaagccat ctccttacat agacttggat caagcaagaa aaaatctttt
    15001 tgatttaagg aaacttggag tgaaagtaat cgactttaca gggggcgaac ccctgcttta
    15061 ccgggaatta tccaccttac ttaaagaagc aaaagattta gggtttgtta ccaccatcac
    15121 caccaactgt cttttatacc caaaacgtgc gtcggaatta agggggttag tagatatgct
    15181 ccatttctcc ctggattccg gagtgaaaca aatgcacgat gagatgaggg gagttgcctg
    15241 ctttgataag gtgatggaat caattgaaat tgcaaaatca ttagaagaaa gaccggatat
    15301 actttttaca gcctttgatc ataacctgga tcaaatagaa aaaatttact acgaaatcgt
    15361 tcttcccaat aacctggtcc ttattcttaa tccatccttt agttacaatc gggttgaagc
    15421 gtctcttgac gaaagaagtt tgaatatact cagtagctgg agtaacaaaa aaaatattta
    15481 tctaaatgat gcgtttattg aacttcgccg ggatggaggg aatcataccg caaacccggt
    15541 ttgtaaggcg gcaagttcta ccattgtaat atctccggaa aataaactga ttttgccttg
    15601 ttaccacttg ggcattcagg aatttaaaat caatggagat ttatttaccc tctatcactc
    15661 ttcagaagtt cagaacctgg tggcattgga aggaagattg ccggaatgtg aaggttgcac
    15721 tattaattgc tatatgcagc cttcatttgc agtagagctg aataagtact ggtggaaagc
    15781 ccttcccagt acattaaaat acaatgcaat aaaaggtacc tggaaaatgg ctctgaaagc
    15841 aatctaatct gtgttttaaa taaaaagagc tgttttagcc tattccatat tattaaatat
    15901 atttgtaggg atgttgatta ggataaaatt tttagaataa tatatgctac ctgttttaac
    15961 ctctattgcg gcctttacag cggttattgt tttactggtt ttggttttgc tttatgctca
    16021 atcaaagttg gtgcagtcag gggatgtcga tataatcatt aatggagatg cttcaaatcc
    16081 tttaacagtt tcagcgggtt caaccctgct taccacgctt tctgctcaga agatattttt
    16141 accatcggct tgcggcggag gcggaacctg tgccatgtgc aagtgccagg tattggaggg
    16201 tgccggagat gtgcttccca cggaggttgg ccatttaagc agaaaagaac agcaagatca
    16261 cgtaaggctg gcctgtcagg taaaagtcaa acaggatttg aaaatcaaaa tcccggagga
    16321 gatattcgga ataaaaaaat gggagtgcga agtagtcagc aattataatg tctctacatt
    16381 cataaaagaa tttgtagtga aattaccgga aggtgagata ctgaattttc aatcgggtgg
    16441 atatattcaa attgatgtac cggaaacagt cgttcatttt aaggagatgg atattactcc
    16501 caaaccggag ttgaatcata agccggacgt atttcagaat gactgggaca aattccaact
    16561 ttggggcctt acaatgaaga atagtgagcc aatattccgc gcatacagta tggctaatca
    16621 tccggcagaa ggaaatatca tcatgctgaa tatacgtatt gcaacccctc cctgggatag
    16681 ggcaaacaat aagtggatgg atgttaatcc gggtatatgc tcctcgtatg tcttttcaag
    16741 aaagctggga gacaaagtaa ccatctccgg accttatggt gagttccata taaacccaac
    16801 ccagcgggag atgatatata ttggtggtgg tgccggaatg gcaccattga gatctcatat
    16861 ttttcatttg tttcacacgg aaaaatccaa cagaaaagtt tcgtactggt atggaggtcg
    16921 atcaaaaaaa gaattgttct acactaatca cttccgatcc atcgaaaaag atttccccaa
    16981 tttctcgttt cacatagccc tgtcggaacc attacaggat gataattgga gcattaagaa
    17041 gtcattggat gataaagatt ccgatggata tatcgggttc atacatcagg tgctttacga
    17101 taattactta aaacttcata aagaacctga agaggttgaa tattatttgt gcggaccacc
    17161 tctgatgaat gccgcagttt tacagatgtt ggatgaaatg ggtataccgt ctgaaaatat
    17221 tcgtttcgac gacttcggtg gttaaaaaaa atatcagggg agatttatct ccctttttta
    17281 ttttactaat ctagtttgct ctcatggatt taaaattatt tttttctcct gcgggaaaag
    17341 aaacttcttc cggaatcaaa tccaaaactg cattgttgaa atacctgcat gtatttcagg
    17401 atgaaatgcc ggaaatcaaa gaggctaata ttgcgttaat cggaataact gaaagtcggg
    17461 gttctgcggg taatgatgga gtaatccggg ctcccgacaa tattcgaaaa gtgttgtaca
    17521 atctgaaaaa gggatttggc aattaccgaa ttgtggattt aggcaatttg agaaacggga
    17581 tgagtttcga ggaaaccaat gggcgggtac gggaagtatg ccagttcctg attcaaaata
    17641 atgtacttcc ggtccttttc ggcggaactc atgctattga ttacggtcaa tatcaggcct
    17701 acgatggact ggaaaaactg gtaacggtgc ttaatgtgga tgcttttctg gatatggaag
    17761 atcccgatgg aaaagaaccg gatcttgatc acaccaatag aatttttttg catgagccta
    17821 attatctttt tcattatagc catcttgcct atcaaagcta tcttgttaat cgtgacacat
    17881 tgtcggtttt agagaagctg tattttgaaa cgcaccgatt gggtaacctg cacacaaatc
    17941 ttgttgaaat ggaaccagtg atccggaacg cggacatgct gacatttgat atatccgcaa
    18001 taaaaagcaa tgatgcaccc gggagtagtc aggctcagcc ttttggatta accggagagg
    18061 aagcttgcca gatttgctgg tatgccggcc taaatgaaaa actaagctct gcaggtttct
    18121 atcaatataa tcctgataaa gacgatgcgg ataacaaaac cgcaaaagtg gtggctacta
    18181 tgatttggta tttcattgag ggttactatc accggcagaa cgaacaaaac ttcaaagcca
    18241 atgattacat gaaatatgtt gtttccatgc ctgctgaccc ggaaacatta atcttttaca
    18301 aaagtaaggc tagcgaaaag tggtggatgg aagttcctat tccaaaaagt ggtggcaaat
    18361 acaaacggga ttacataatt ccctgcacgt attcggatta tgaaaaatca acttccggtg
    18421 atgtgcctga cagatatatt tccactctgg cgaaattggt ttaaactgat cttcagttta
    18481 ttgaagtaag aagaaattca tttccctttt taataagaaa gtcaggcaat tttatgccag
    18541 ccctcttagc acaaatttct tcagcgccgg ttttaataga ctttaatcac ttttctggta
    18601 attctgccct tgggtgtttc taatgtaatt atgtacaatc catcaggaaa attacctaaa
    18661 tcaataactt cattcacgat tgtatttctt gagtccagct gcttcaggaa aatacttttt
    18721 cccaataaat tggttacgtg tatcctggag ttgctgattg gctcgtttaa ttgtgccttc
    18781 agagtaaaat taccacttga tggattcgga tatatcacaa atgactcctc atcaataact
    18841 tcatcaattc cggtaatcaa actttgaatg cgcaggttat cgattaccca gccccagcca
    18901 acggctaact catcagacca aagccgaaag cggattctca ccgtttcgtc agccgaaaaa
    18961 ttaccattgg cgagcatgtc tatcttacga tctgcataca gatcggaatc gccgctggct
    19021 ccggaattaa gagttgatgg atcaaaatcg ct
//
LOCUS       DASWHX010000207         4728 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_964100, whole genome shotgun sequence.
ACCESSION   DASWHX010000207 DASWHX010000000
VERSION     DASWHX010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 4728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4728
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_964100"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(299..2713)
                     /locus_tag="VGA21_13720"
     CDS             complement(299..2713)
                     /locus_tag="VGA21_13720"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9651615.1"
                     /translation="MIRSIFISAIRNIRRHRTFSFINVFGMIISLSLSLLIIQVVVDQ
                     YSFDTFHEDSDRIYRVNSEIFRTGGGTEKYATTPLPLTDKLSSDFTFFEEVTRLSGGL
                     SGNVTSSGQTIPIRGFYTSPTFFKIFNFEFENSVAEPLKDPFQIVLTRNAAHKFFGRE
                     SALGKSLVIEGFGTFTVSGVLKDSNKNTHLEFEALASQSTLHSLRQTSAVRPQMFEWK
                     NYYSAYSYLKIKEGVRVEDIEAALTAIEEENYKEVILDARDQKITFYLQALKDISPGP
                     QLSNNLGRSLPDILLYFLIGLAAIVMAMACLNYTQLTIAKSITRAREIGIRKVIGAQK
                     SQVFLQFVGEAVVISLVSLVFSYLLLQFIKPAFLTLHISNEFSFNLNESPGVLLLFVI
                     FTTVIGIIAGSFPALYLSSLKPVHVLKSGNKLNNNSKLPLRKAMLTLQFTFAVIFTLT
                     VLIINKQTQYMIKADYGINTDNIINVRLNGISFDQIAPQVANLSGVEGVAGVSHSLGT
                     FEDGDSDYKKSPEAEPFNMRDFMITGLYLQNLDIKLLAGKYFSEEMLTAGGEKFVILN
                     EKAVRLFGFADNSASLDEVIYVDDSVMLEVVGVTESFHFRPFNYEIGPIAFRLRPDAA
                     RILSIRFQPGTNPEQLASAVLPVYRKFLPNHEMEWSLMDNEIKDAYEDSGLTDISKIV
                     GYISFLAISISCLGLLGMVMFIVQTRSKEIGMRKILGASVSQLVIILNKSSMIILAIA
                     FAIGVPVSYLLGDWFLSIYAYRIPIGLSLILTGVGVIMGIVFITIGSQTLPTAKSSPL
                     KWIRYE"
     gene            2895..3296
                     /locus_tag="VGA21_13725"
     CDS             2895..3296
                     /locus_tag="VGA21_13725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651616.1"
                     /translation="MHLTIALIIPVLLVYSFEVKSQEYHKAVAKLNSIYEVHGLSSVS
                     EPIVFDSLTRILEIDNYRVPMSDNTFIQCVKNRRAKDGMKYSAKFYMQQGETITLKGN
                     DLIQKAFLAIPLSDQGICKAFVFQLEQLKKE"
     gene            complement(3412..4434)
                     /gene="aroF"
                     /locus_tag="VGA21_13730"
     CDS             complement(3412..4434)
                     /gene="aroF"
                     /locus_tag="VGA21_13730"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014021132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEX9651617.1"
                     /translation="MIIKLTDEINRKEKASIISKVSDLNFKTREVKTQKSEYLIGIGT
                     AEFDIREIGHLPGVEDIHVVSDNFKLVSRKWKVKTTTIHLGHDISIGRENFSVMAGPC
                     AIEGEKQILETLSHLRNNGVKIMRGGVFKPRSSPYSFRGLGMDGLKLWYEAAKEYGIK
                     IITEVMQVSQVEEMYDFVDIYQVGARNTQNFNLLDELGKVDKPVMIKRGISGTIDELL
                     YSAEYVFSGGNENLILCERGIRTYETASRNTLDLNAIPILKDKTHLPVIADPSHGIGI
                     RKYIEPLALASIMAGADGVIYELHKTPEEAASDGYQSLNFFESEELIRKMRKVYEMKR
                     QLNQNH"
     gene            complement(4415..>4728)
                     /locus_tag="VGA21_13735"
     CDS             complement(4415..>4728)
                     /locus_tag="VGA21_13735"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF012511.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEX9651618.1"
                     /translation="RLIDSISSSFIYAVSDSAITGVKKDLTEHQLQYLKRLSEMNLKH
                     PVLTGFGISNAAAFQSVCKFVSGGIIGSAFIRELSNGTDIRNSIANFLTQFKANDHKI
                     N"
BASE COUNT         1431 a          987 c          894 g         1416 t
ORIGIN      
        1 acaaccggaa caatgtacga cacccagcct gccgcactca gaatgaaagg catcatggaa
       61 ggcaaaggtt atcctttact ttatattgaa gttaaccagg gccacagttg gggaaactgg
      121 gaagaattac tggatgatgc gcttgtttat ctttatggga attgaaaaaa gattacagta
      181 ttgatgaaaa accaaatttt cacctaaaat aagtggaagt gatccgacca ctgggtgtgt
      241 gttccttact tctaaacgtc agtgttgaaa atttggtaag gacatgctca ttcaggattc
      301 actcatagcg aatccatttc aacgggcttg actttgccgt aggcaaggtc tgtgaaccga
      361 tggtaataaa tactattccc ataattacac caacgccggt taaaatcaag ctcagaccaa
      421 ttggaattct gtaggcataa atgctaagaa accaatcacc caaaaggtaa cttaccggga
      481 caccgatggc aaaagcaatc gccagaatta tcattgatga tttattaaga atgataacca
      541 gttggcttac gctggcacct agtattttcc gcattccaat ttctttggaa cgggtttgca
      601 cgatgaacat aaccatcccg agaagcccaa gacaggaaat gctgatagcc aaaaatgaaa
      661 tatatcctac aatcttgctt atgtcggtta atcctgaatc ctcataggcg tctttaattt
      721 cattgtccat cagagaccat tccatttcat gattaggcag gaatttccgg taaacaggca
      781 gaactgcaga cgcaagttgt tcaggatttg ttccaggttg aaaacggatg ctgagaatcc
      841 tggctgcatc gggccgaagc cggaatgcaa taggaccgat ttcataattg aatggtctga
      901 agtgaaagct ttctgttact ccaaccactt ccagcataac agagtcatcg acataaatta
      961 cttcatccag cgatgctgaa ttatctgcaa agccaaataa tcgtaccgct ttttcattca
     1021 ggattacaaa cttctccccg ccggcggtta acatttcctc cgaaaaatac ttacccgcaa
     1081 gcaatttgat atccagattt tgaagataca aaccggtgat cataaaatct ctcatattga
     1141 aaggctccgc ttccggagat tttttataat cgctgtcgcc gtcttcaaaa gttccaaggc
     1201 tgtgtgaaac acccgctacg ccttccaccc cacttagatt agcaacctgg ggagctattt
     1261 gatcaaaaga aatcccattt agtctgacat taataatatt atcagtgttt attccataat
     1321 cagctttaat catatactgg gtttgcttgt tgataattaa aacggtaagc gtaaaaatca
     1381 ccgcaaaagt aaactggagt gttaacatcg ctttccgcag cggaagtttc gaattgttgt
     1441 tcaatttatt ccctgatttt aatacatgaa ccggcttcag agatgacaaa tacagcgctg
     1501 gaaatgaacc tgctattatg ccgataacag tggtgaaaat aacaaagagt aaaagtaccc
     1561 ctgggctttc attcaaattg aatgaaaatt cattacttat atgtaatgtc aaaaatgccg
     1621 gcttaataaa ttgaagtaat aagtaagaaa acaccaggga aaccagagaa attaccacgg
     1681 cctcgcccac aaattgcaaa aacacctgac ttttttgggc gccaattact tttctaattc
     1741 ctatttcacg tgcccttgtg atagattttg caatggtgag ttgggtataa ttaagacagg
     1801 ccatcgccat tactatagct gccaaaccaa tcaggaaata caaaagaata tctggtaatg
     1861 atcgccccag attgttagaa agctgaggtc ccggactaat gtctttgagg gcttgcaggt
     1921 aaaaagtaat tttctggtcg cgcgcatcaa ggattacttc tttataattc tcttcctcaa
     1981 tggctgtcaa agcagcctca atatcttcaa cacgtactcc ttcttttatt ttgaggtaag
     2041 aatatgcgga ataatagttc ttccactcaa acatttgcgg tcttaccgcg gatgtttgtc
     2101 ttagggagtg caatgtggac tgagaggcaa gtgcttcaaa ctccaggtga gtgtttttgt
     2161 tagaatcttt cagcactccg gatacagtaa aagtaccaaa gccttcgatt accagagatt
     2221 ttcccagggc ggattcgcgg ccaaagaatt tatgtgctgc attcctggta agcacgattt
     2281 gaaatggatc tttaaggggc tctgccacac tgttttcaaa ctcgaaattg aatatcttaa
     2341 aaaatgttgg cgaggtataa aatccccgga ttggaattgt ctgccctgaa gaagtaacat
     2401 tgccactgag ccctccggat aatcgcgtca cttcttcaaa aaaagtaaaa tcactgctca
     2461 gtttgtctgt caatgggagg ggagtagttg cgtatttttc agttccgcca ccggtgcgaa
     2521 aaatttctga attaacccgg taaattctat ctgaatcctc atgaaaagta tcaaaagaat
     2581 attggtcgac tacaacctga attatcaaca gactgaggga taaactgatg atcattccaa
     2641 atacattaat aaatgaaaat gttctatggc ggcgaatatt ccttatcgcg gagatgaata
     2701 tacttcgtat catggtttgg ggatttaggt tcaaaggaaa ccaaaaagaa tgccaccctc
     2761 aaggaattgc caggattaag atccggtaag aatgaattga ccgttgacgc tcaaaactgt
     2821 tcgctcccga acggaatatc atggtaattc ctaaagtaat ttgctaattt tgaaattact
     2881 tataataaaa tagaatgcat ctgactatag cgctcataat tccggtgttg cttgtttatt
     2941 catttgaggt caaaagtcag gaataccaca aagcggtggc aaaactcaat tcaatttatg
     3001 aggttcacgg attaagttcg gtcagcgaac ccattgtatt tgatagtttg accagaatcc
     3061 ttgaaataga taattacaga gtgcctatgt cggacaatac ttttattcaa tgtgttaaga
     3121 acaggagagc gaaagatgga atgaagtatt ccgcaaagtt ttatatgcag cagggagaga
     3181 ccatcaccct aaaaggaaat gatttgattc aaaaggcatt ccttgccatt ccattatctg
     3241 atcaaggaat ttgcaaggcc tttgtattcc aattggaaca gctcaagaag gagtagattt
     3301 accgcttaac ttccacatca tttttaaccc tgagccagtt tccccaagcg tccgttaatt
     3361 taattattga taaattgaag ggcctccctt gcgaggtaat cttattctgt tttaatggtt
     3421 ctgattgagt tgtcttttca tctcataaac tttccgcatc tttcttatta attcttcgga
     3481 ttcaaaaaaa ttcagacttt gatagccatc agatgcggct tcctccggag ttttatggag
     3541 ttcataaata actccatcag ctcctgccat tatgcttgcc aaagccaggg gctcaatata
     3601 ttttctgatt ccaatgccgt gggatgggtc ggcaattaca ggcaaatgag tcttgtcttt
     3661 aagaatcgga attgcattga gatcaagcgt atttctactc gctgtctcat aagtccttat
     3721 tcctctttcg caaagaataa gattttcatt cccgcctgag aatacgtact cagctgaata
     3781 caggagttca tctattgtgc cgctaattcc tctctttatc attaccggct tatccacttt
     3841 tcccaattca tccaataaat taaaattttg ggtgttcctg gcacctacct ggtagatatc
     3901 gacaaaatca tacatttcct ccacctggct tacctgcatc acctcggtga taattttaat
     3961 accgtactcc tttgccgctt cgtaccataa ttttaaacca tccattccca aacctctgaa
     4021 ggaatatgga gaactacgtg gcttgaatac tcccccacgc attattttaa ctccattatt
     4081 tcttaaatga cttagcgttt ccagaatttg tttttcccct tcaatggcac acgggccggc
     4141 cattactgaa aaattttctc ttcctatgct aatatcatgt cctaaatgta tggtagtagt
     4201 ttttactttc cattttctgc tcaccagttt gaagttatca gacacaacat gaatatcttc
     4261 cacgccgggc aaatgaccaa tctcccggat gtcaaactct gcggttccaa ttcctattag
     4321 atattcggac ttttgagtct taacttccct ggttttgaag tttaaatctg aaactttgct
     4381 aatgatggat gccttttcct tccggttaat ttcatcagtt aattttatga tcatttgctt
     4441 tgaattgagt taaaaaattg gctattgaat ttctgatatc agttccattt gacaactccc
     4501 ggataaaggc gcttccgata attccaccgg aaacaaattt gcaaacactc tgaaaggcag
     4561 cagcatttga aataccaaac cctgtcaata cgggatgttt cagattcatt tcagaaagtc
     4621 ttttcaagta ttgcaattga tgttctgtta aatctttttt caccccggta atggccgaat
     4681 cagaaacagc ataaataaag gaacttgaga tagaatcgat taatcgaa
//
LOCUS       DASWHX010000208         5430 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_1332905, whole genome shotgun sequence.
ACCESSION   DASWHX010000208 DASWHX010000000
VERSION     DASWHX010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 5430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5430
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1332905"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..704)
                     /locus_tag="VGA21_13740"
     CDS             complement(<1..704)
                     /locus_tag="VGA21_13740"
                     /inference="COORDINATES: protein motif:HMM:NF018190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate binding family 9 domain-containing
                     protein"
                     /protein_id="HEX9651619.1"
                     /translation="MKTHTTATRILFLFLIFGFSGVHARQVEKKSMEARRVDHPPKID
                     GVLEEIWEIESTERLKFYQSQPDNGKAASFDTQVLVLYTNYGLYIAAKMFDDDPRNIK
                     KELGERDNMDKNTDQFGVLFDTYNTGQNAFGFVVNASKVQSDAFLTPNNDDLSWDAVW
                     TSAVGFDEEGWIVEMEIPFSAIRFPKKEEPVWGINFMRQIKDKAEESWWNFIDNSIEG
                     FVNQSGELKGLKDISPP"
     gene            814..1311
                     /locus_tag="VGA21_13745"
     CDS             814..1311
                     /locus_tag="VGA21_13745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013452917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEX9651620.1"
                     /translation="MIIGLISDTHGHVDDKVFNHFKDVDEIWHAGDIGDKATLRKLTD
                     FKPVKAVFGNIDPNELQLTLPEDLWFTCNGLEVMITHIGGYPPKYNKRIRKILEETRP
                     GLFICGHSHILKIMTDAERDNMLYINPGAAGNQGFHLIKTLVKFSIENSKLTKLQVIE
                     LGRRG"
     gene            complement(1341..1568)
                     /locus_tag="VGA21_13750"
     CDS             complement(1341..1568)
                     /locus_tag="VGA21_13750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651621.1"
                     /translation="MAITRLKRKGRRNILKAKNKRKAIKRLKAVPVIRNIDVEAIKKE
                     FSKKSSKVEEKEATAEVKPDKKPKAVTKKKN"
     gene            1724..3133
                     /gene="hisS"
                     /locus_tag="VGA21_13755"
     CDS             1724..3133
                     /gene="hisS"
                     /locus_tag="VGA21_13755"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014770743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEX9651622.1"
                     /translation="MAREKPGLPKGTRDFGPDVMNKRNFILNTITKVFRKYGFRQIET
                     PSLENLSVLMGKYGEEGDQLLFKVLNSGDYLKDVTDEDFEKGYKSLLPKITNKGLRYD
                     LTVPLARFVVMNRNEISFPFKRYQIQPVWRADKPQRGRYREFLQCDADIIGTSSLLCE
                     MEIVQMMAEVMSELGIAGFKIRMNHRKILGSMAKSDGLQNQENLFAVIIDKMDKIGWD
                     GVSKELLKIGFTPDSLSKFKQIFSGELDKEDLRKKLSPMLDKDGANGLDDIFTVFEML
                     KKSRGNPDEQDFKWHLQFDPSLARGLSYYTGIIFEVEIEGVTVGSVSGGGRYDDLTGI
                     FGLPDVAATGFSFGIDRLYDALEELKVFPDFAIEGHNILIVHFDEETFNYGLEVLWRL
                     RKNQKLNTRGVSIEMYPDQSRLTKQMAYANRNPIRFTIIIGSEEMKSGKLTLKDMITG
                     DQQSLSEDEIIDYLSQKRV"
     gene            3130..4620
                     /gene="hutH"
                     /locus_tag="VGA21_13760"
     CDS             3130..4620
                     /gene="hutH"
                     /locus_tag="VGA21_13760"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009581557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HEX9651623.1"
                     /translation="MKKIHTISLEDIGLNQMSECLENDISLELSPEAINKINRCRDYL
                     EKKLRETTGPIYGINTGFGSLYDRDISNENLGKLQENLIKSHACGTGKPIEKEIVQLM
                     LYLKIKSLSYGHSGVRLETVNGLIDFFNNRIYPEVFTFGSLGASGDLAPLAHLSLPLI
                     GLGNVYFENERYTGAEILKIMRWDALGFREKEGLALLNGTQFMSAFGCYCLIHAERLI
                     RLANIIGALSLDSFDGRIEPFHPSVHKIRPQPGQSVVAAQVRDLLSDSQLIRLSKKHV
                     QDPYSFRCIPQVHGACLDTFEFAKNIFLSEINGVSDNPNIFPDEDLIISAGNFHGEPL
                     AFALDFLSIALAELGNISERRTFQLISGARGLPDYLVANPGLNSGLMIPQYTAAALVN
                     RNKLLANPASTDSIVSSNGQEDHVSMGANAATKAYEIVQNLYSILAIELMTASQALEF
                     RRPSRTSSYLEDWIHNFRKNVPFIDQDRVLHEDIEKSEAFLRKATI"
     gene            4633..>5430
                     /locus_tag="VGA21_13765"
     CDS             4633..>5430
                     /locus_tag="VGA21_13765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HEX9651624.1"
                     /translation="MAEKKQILGIFLLALTFNMARAQNASPLGRFSVDFIKGCAPLTI
                     LVTDNLSELTPIYQYKGFNSNATNQTTYTYNTPGQFYIVQTIQIPTDRQDSLLIQVME
                     PVQPEFTLFTCETQGASVQVTDNYYDQYEIDYGDGTIVTVNSSVPITNHFYNSAGTYT
                     ITVRGIFTNAFDNCGISSKSFTTISNISPAIISLLEMQTNGDIILNYTLPQSILYDIQ
                     IAVENGNIFQKLKSISNQSSKDTIRNLNPSRNYCFRIISYDPCVNNTI"
BASE COUNT         1610 a         1054 c         1123 g         1643 t
ORIGIN      
        1 ggcgggctga tgtcctttaa cccttttaac tctccggatt gatttacaaa tccttcaatg
       61 gaattgtcta taaagttcca ccaggactcc tcagctttgt ccttaatttg ccgcataaaa
      121 ttaatacccc acacgggctc ctcttttttg ggaaaacgaa ttgcactgaa aggaatttcc
      181 atttcaacaa tccagccttc ttcatcaaaa cccaccgcac tggtccatac cgcatcccag
      241 cttaaatcat cattattggg tgttaaaaag gcatctgact gaactttgga ggcattcacc
      301 acaaatccaa atgcattctg tcccgtatta tatgtgtcaa ataagactcc aaattgatct
      361 gtatttttat ccatgttatc ccgttcacct aactcctttt tgatattcct gggatcatcg
      421 tcaaacattt tggcggcgat atacaaacca taattggtat ataacacgag gacttgagtg
      481 tcaaatgatg cagctttacc attatctggt tgggattggt aaaactttag tctttcggtg
      541 gattcaattt cccatatctc ttccaaaacg ccgtcaattt ttggtggatg gtcaactctt
      601 cgagcttcca tcgatttttt ttctacttgc cgtgcgtgta ctccggagaa tccaaaaata
      661 agaaacagga atagtatcct tgtggcggtt gtgtgggttt tcaaaagaat ttgagtattt
      721 gtcctatgtt gttaataccg gtaaaacgcg aaaaggttgc aaaaggatct gctatttgga
      781 cattttatta tataattccc taaaaattct gttttgataa ttggcttaat ttcagatact
      841 catgggcacg ttgatgacaa agttttcaat catttcaaag atgttgatga aatctggcat
      901 gccggagata tcggcgacaa ggcaaccttg aggaaattga ccgattttaa acctgtgaaa
      961 gcggtttttg gtaatattga tcccaacgaa ctccaactta cgcttccgga agatctttgg
     1021 ttcacttgca atggattaga ggtgatgatt acccacattg gaggatatcc gcccaaatac
     1081 aataaacgga ttcgaaagat tttggaagaa accagacccg ggttatttat ttgcggacat
     1141 tcacatattc tgaaaataat gacagacgcc gaaagggaca atatgttgta tataaatccc
     1201 ggtgctgccg gaaatcaggg ctttcacctg attaaaaccc tggttaaatt ttcaattgaa
     1261 aatagtaaac ttactaagct gcaggtaatt gagcttggcc ggcgaggata aaaaaagcct
     1321 cagttggagg ctggagatta ttagttcttc tttttcgtta cggctttggg cttcttatcc
     1381 ggctttacct ctgcagtcgc ttctttttcc tcaacctttg aggatttctt tgagaattct
     1441 ttcttgatag cctccacatc aatattcctg ataacgggaa ctgccttcaa tcgctttatg
     1501 gctttccgct tattttttgc cttcagaatg tttcttctgc cttttctttt cagtctggta
     1561 attgccattg tattgttttt tgaggatgta aatctaatgc atatcgaatt tattaataaa
     1621 taaattgacg tgaatttctt tagttgatat attcctaaag caaaggatgt agattgaata
     1681 tgctcaattt gtttagcttt gcgtgccacg caacaagatt ttaatggctc gagaaaaacc
     1741 tggattgcct aaaggaaccc gcgacttcgg acctgatgtg atgaataaga gaaattttat
     1801 tctgaatacg attacaaagg tttttagaaa gtatggattc aggcaaattg aaactcccag
     1861 tttggaaaat ctttcggtgc tgatgggaaa atatggagag gagggagatc aactgttatt
     1921 caaagttctt aattccggag attatctgaa agatgttact gatgaggatt ttgaaaaagg
     1981 atataaatcc cttcttccca aaattacaaa taaaggatta agatatgatt taacagttcc
     2041 cctggcccgc tttgtggtga tgaacaggaa tgaaatctcg ttcccattta agagatatca
     2101 gatacaaccg gtctggcggg ccgacaaacc tcagcgtggc aggtataggg agtttttaca
     2161 gtgcgatgcg gatattatcg gcacctcaag tttgctctgc gaaatggaaa tagttcaaat
     2221 gatggcagaa gttatgagcg aattgggaat tgccgggttc aaaatccgta tgaatcacag
     2281 gaaaattctt ggcagtatgg ccaaatcgga tggattacaa aatcaagaaa atttgtttgc
     2341 ggttattatt gataaaatgg ataaaattgg ctgggatgga gtttcaaagg agttgctgaa
     2401 aataggtttt actcccgatt cactttcaaa gttcaagcaa atcttttccg gagaattaga
     2461 taaagaagat ctgagaaaaa aactatctcc catgcttgat aaagatggcg cgaatggact
     2521 tgacgatatt tttacggtgt ttgagatgct gaagaaatcg aggggaaatc ctgatgaaca
     2581 agattttaag tggcatcttc agtttgatcc ttcactcgcc cgcggattaa gttattacac
     2641 tgggataatt tttgaagtcg aaatagaagg agtaactgtg ggaagtgttt ccggcggcgg
     2701 aagatatgat gacctgaccg gtattttcgg tttacctgat gttgcagcca ccggcttctc
     2761 atttggtatt gacaggcttt atgatgcgct ggaagaattg aaagtatttc cggattttgc
     2821 cattgagggt cataatatcc tgattgttca ttttgatgaa gaaacattta attatgggct
     2881 tgaagttctc tggaggttaa gaaaaaatca gaaactcaat acgcgtggtg tttctatcga
     2941 aatgtatccg gaccagtcca ggttgaccaa acaaatggct tatgctaacc ggaatcccat
     3001 tcgatttacc attattattg ggtctgaaga aatgaagtcc gggaaactta ctttaaaaga
     3061 tatgattacc ggggatcagc aatctctttc tgaagatgaa attatcgatt acttaagcca
     3121 aaaaagggta tgaagaaaat tcataccata tcattagagg atataggcct gaaccaaatg
     3181 tcggaatgtc tcgaaaacga tatttctctg gagctttctc ctgaagccat taataaaatc
     3241 aatcgatgcc gggactatct ggaaaaaaaa ctccgcgaaa ccactggtcc catctatgga
     3301 atcaacacag gctttggctc gctatatgat agggatatat cgaatgaaaa cctgggtaaa
     3361 ttgcaagaaa atctaatcaa atctcatgct tgcggaactg gaaagcccat tgagaaagag
     3421 attgtccaat taatgcttta tttgaaaatc aaatccctgt cctatggcca ttccggagta
     3481 agactggaaa cagttaacgg gcttatagat tttttcaaca acagaattta tcctgaagtt
     3541 ttcactttcg gatccctggg tgcctccgga gatttagctc ctctcgcgca tctttcacta
     3601 cccttgattg gtttgggaaa tgtttatttt gaaaatgaga gatataccgg tgcagaaatt
     3661 ctgaagatca tgcgatggga tgctttggga ttccgggaaa aggaaggttt ggcattgctc
     3721 aatggtactc agtttatgag tgcttttggc tgctattgtc tgattcatgc ggaacgactt
     3781 atccggctgg cgaatattat tggcgcactc agtctggata gcttcgatgg caggattgaa
     3841 ccgttccatc caagcgtgca caaaatacgc ccccagcctg gccagagtgt agtggcagca
     3901 caggtacggg acttgttatc cgacagccaa ttaatccgcc tgtcaaagaa acacgtgcaa
     3961 gacccttaca gctttcgctg cataccgcaa gtacatggag cctgcctgga tacctttgaa
     4021 tttgcaaaaa atattttcct ttctgaaatc aatggagtat cagacaatcc caatatcttt
     4081 ccggatgaag atttaattat ttcggccggt aactttcatg gtgaaccgtt ggcatttgct
     4141 ctggattttc tcagcattgc tcttgctgaa ctggggaata tttcagagcg aagaacgttt
     4201 caactgattt ccggagccag aggcctgcct gattatcttg tcgcaaaccc cggcttgaat
     4261 tccggattga tgatcccgca atatacggcg gcagcacttg ttaacagaaa caaattattg
     4321 gctaatcctg ccagcaccga cagcattgtt tcttcgaacg gacaggaaga tcatgttagc
     4381 atgggggcta atgctgcaac aaaagcctac gagattgttc aaaatcttta ttccatactt
     4441 gccattgaac ttatgactgc cagccaggca ttagaattca gaaggccttc caggacatcg
     4501 tcttatcttg aagattggat tcacaatttc cggaagaatg taccgtttat tgatcaagac
     4561 agggttttac atgaagatat cgaaaaatcg gaggcatttt tgaggaaagc taccatttga
     4621 tacttccata ttttggctga aaagaagcaa atcctgggta tttttttatt ggcgctcaca
     4681 ttcaacatgg ccagggcgca gaacgcaagt cccctgggaa ggttttccgt ggactttata
     4741 aagggatgcg caccgttaac gattcttgta acggataatt taagcgaatt aactcccatt
     4801 tatcagtata aaggctttaa ttcaaatgca acaaatcaaa ccacttatac ctataataca
     4861 cccggtcaat tttacattgt tcaaaccata caaatcccaa ctgatcgcca ggacagtctc
     4921 ctgatccagg taatggagcc ggtacaaccg gaattcacgc tttttacctg tgaaacccag
     4981 ggagcctccg tacaggttac cgataattat tacgatcaat atgaaatcga ttatggagat
     5041 gggacaattg tgacggttaa ttcgagcgtg ccaattacga atcactttta taattcagca
     5101 ggcacgtata ctataacagt tcgaggtatt tttactaatg cttttgataa ctgtggcatc
     5161 agttctaaaa gttttactac catcagtaac ataagtcctg ccataatttc gcttcttgaa
     5221 atgcaaacca atggagatat aatcctcaat tatactctgc ctcaaagtat cttatacgac
     5281 attcaaatag ctgtggaaaa tggaaatatt tttcaaaagt tgaaatccat ttcaaatcaa
     5341 tcctcaaaag ataccatcag gaatttaaat ccatcaagaa attattgttt ccggataatt
     5401 tcctatgacc catgtgttaa caatacaatt
//
LOCUS       DASWHX010000209         7813 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_1725467, whole genome shotgun sequence.
ACCESSION   DASWHX010000209 DASWHX010000000
VERSION     DASWHX010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 7813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7813
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1725467"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..675
                     /locus_tag="VGA21_13770"
     CDS             <1..675
                     /locus_tag="VGA21_13770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651625.1"
                     /translation="NQGNQSSENNRVTICHKTPAGNNSITVSQAAVETHLRHGDSLGA
                     CQDEQGSPENQNQENRNQGDQNQSDQGNQVQKDGGLNKEGSGSQVNQGSSDRDNSGTE
                     GSRSTQGNSGAEGSSEVPVYGTVKATLYKFTKTISSGGVLDFRVRDAFTNQVITQEKL
                     PGEFIWTSEWATFNGDERALRPEHVDMIKLREAPAPPPQTLFVEFTKPIYDQIINKIV
                     AYYRNF"
     gene            772..1872
                     /locus_tag="VGA21_13775"
     CDS             772..1872
                     /locus_tag="VGA21_13775"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008626056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HEX9651626.1"
                     /translation="MSTSTNKIQFNTQSDFYRELIRNVNDYFKANNISKYGNRRMIIK
                     TICMFVFYFAPFSLLFLDFGFGLWSVLVLYALMGIGKSGIGLSVMHDANHNSFSKNQK
                     VNKWMSYSMNIVGGSSINWKIQHNVLHHTFTNVHGHDEDINAPFILRFSPHQKYYLIH
                     KFQFIYSWFFYGFLTLGWIMYQDYGQLIGYNKRGLLKSQKTTFSKEFTILILTKISYL
                     AYIIALPMIFGPAVWWSYLVGFAIMHYVAGFILSIIFQPAHVIEETEFPLTDESGKLA
                     TGWAEHQLMTTCNFARNSRIFSWFVGGLNFQVEHHLFPNICHVHYPGISAIVKATAEK
                     FNLPYYSQRTFLSALVAHGKMLYRLGNPKLAA"
     gene            1968..3644
                     /locus_tag="VGA21_13780"
     CDS             1968..3644
                     /locus_tag="VGA21_13780"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015029899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HEX9651627.1"
                     /translation="MKKQYLFLLAIIILAACKPSIKYDVIIRNGTVYDGSGGDPVVTD
                     IGIVGDSIVALENLDNISAATEIDAIGMAVAPGFINMLSWATTSLIEDGRSLGDIKQG
                     VTLEVFGEGWSEGPLNDAMKEEITKDQGDIKYDIKWTTLGEYMDYLVEKGVSCNIASY
                     VGATTLRIHELAFENRSPTPEELERMKNLVRTAMEEGALGIGSSLIYAPAFYADTEEL
                     IELCKVASEYGGSYISHMRSEGDYFLEAIDELMRISKEANLPAEIYHLKAGGKENWHK
                     MDLALAKIDSARNAGLAITADMYNYIAGATGLDASMPPWVQEGGYDEWSKRLKDLEIR
                     KKIKGEIENKGAGWENLYYAAGTPDNLLLVGFKEDSLKYLTGKTLTEVAQLRNQDPVE
                     TMMDLVIQDGSRVGTVYFLMNEENLVKQIKKDYVSFCSDAESEAPEGVFLKSNPHPRA
                     YGNFARLLGKYVREENVISLQEAIRRLTSLPASNIKIKRRGSLAPGFKADVVIFDPNE
                     IRDHATFEKPHQYATGVRDVFVNGIQVLSGGEHTGNFPGRVVRGPGWKGQ"
     gene            3651..4403
                     /locus_tag="VGA21_13785"
     CDS             3651..4403
                     /locus_tag="VGA21_13785"
                     /inference="COORDINATES: protein motif:HMM:NF023828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3667 domain-containing protein"
                     /protein_id="HEX9651628.1"
                     /translation="MNCKNCAVEFEGNYCPNCGQNSGVMRFTYPYFLRETFFSSLDIE
                     HGFTYSLKALFLRPGNAIRDYLQGKRLSLTVPGKYLILMGAISTFLAVQYEIFKVPEI
                     SEKLMWLPDVAGFFKFAAQYTTVINIITIPIFAFFSWIIFRNKGYNYAENTILNIFIT
                     SQQLLMLVILVPLVSLFETMNSELVACYTIITIIYNVWVYLQFFKLKIVSGTLLSLAA
                     ILLSYAGQFFFNYFIYTILPQQWVEYLAFITL"
     gene            complement(4393..5316)
                     /locus_tag="VGA21_13790"
     CDS             complement(4393..5316)
                     /locus_tag="VGA21_13790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010611832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HEX9651629.1"
                     /translation="MKRRTFFNNVAALSTLAVTGIPANALFADSFQKKNSFNLKYAPH
                     FGMFRESAGEELTDQLQFIADQGFTALEDNGMMDRQVEDQERIAKKMSTLGMTMGVFV
                     AHKIYWSEPNLASGKPDKLEEFLDYIKKSVEVAKRVNAKWMTVVPGHLDLRLELDYQN
                     ANIINALRRACDILEPHNLVMVLEPLNRFNHPGLFLTKIPQAKMLCQAVDSPSCKILF
                     DIYHQQIQEGNLIPNMEMAWDDIAYFQVGDNPGRKEPGTGEINYQNVFQHIYQKGFRG
                     VVGMEHGNSVNGKDGELKVIEAYKRADNFKV"
     gene            complement(5319..6662)
                     /locus_tag="VGA21_13795"
     CDS             complement(5319..6662)
                     /locus_tag="VGA21_13795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020528816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX9651630.1"
                     /translation="MKKENPISPIKRRTFMKGSAMAAGAVMLDPFSSIASHFNTSVDD
                     TIKVALIGCGGRGTGAANQAMKTKENVKLVAMADVFEDRLKGSFETLTKVHGESGRMA
                     VPEENKFLGFDSYKKAIDLADVVILTTPPGFRPWHFEYAVQQGKHIFMEKPVATDAYG
                     IRKVLENAEIAKAKKLNVVVGLQRHYQSNYREAVKRINDGMIGDIISGQVYWNDGGVW
                     VNERKPGMTEMEYQVYNWYYFTWICGDHILEQHIHNIDVANWFIGEYPVRAQGMGGRQ
                     VRTGKEYGEIFDHHFVEFEYPGGAIISSQCRHQRDCMSRVSETFQGTKGSVYTNSNNK
                     ALLKNYPNSILYEHAGEGDPNPYQVEHDELFAAIKEGKVIINDAENGAKSTMTAIMGR
                     MATYSGKVMNWDEALASNKRLVDDPEQWSWKSQPPTLPDENGFYPIPAPGQTEVL"
     gene            complement(6670..7617)
                     /locus_tag="VGA21_13800"
     CDS             complement(6670..7617)
                     /locus_tag="VGA21_13800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009185031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HEX9651631.1"
                     /translation="MKLLKFLLFPLIFVLPAYIFNGFVTYKLTIPNSNESIQFVAIQG
                     GKFTMGNNAIPTESPEHVMEVSDFWMSVHEVTWQQYELFVRRDKNNADPISNEKLKEL
                     GIDGVSRATQPYTDMSFGMGKEGFPAVNITQYAALSYCKWLSALTGDFYRLPTEAEWE
                     YTAKGGVLHDYFTVINSLDDYAWHKGNSSSHYHAVGLKKPNSLGLYDILGNVSEWTMD
                     AYAPYENKSTKDFWRIPQELYPRVARGGSWMDERSKITPTHRIDSQPGWKIRDPQLPK
                     SRWWHTNAPFIGFRIVRPRVQPTPEEIIKYWLKPIEDYN"
BASE COUNT         2184 a         1641 c         1642 g         2346 t
ORIGIN      
        1 aaccagggaa accaatcttc agaaaataac cgggtaacta tctgtcacaa aactccagcc
       61 gggaataatt cgattactgt ttctcaggca gcagttgaga cacaccttcg acatggtgac
      121 agcctgggag cttgtcagga tgagcaaggc agcccggaaa accagaatca ggaaaaccgg
      181 aatcagggag accagaacca aagtgatcag gggaatcaag ttcagaaaga tggcggcctg
      241 aacaaggagg gcagcgggag ccaggtaaat caaggaagct ccgaccggga taattctggc
      301 actgagggaa gcaggagtac gcagggtaat tccggtgcag agggttcatc ggaggttcct
      361 gtttatggca ccgtaaaagc cacactatat aaatttacca aaacaatttc ttcaggcggg
      421 gttcttgatt tcagggtaag ggatgcattt accaaccagg ttatcacgca ggaaaaattg
      481 ccgggtgagt ttatctggac ttcagaatgg gcaactttca atggcgatga aagagcgctt
      541 agacctgaac acgtcgatat gattaagctg cgtgaagctc cggcgccacc gccccagact
      601 ctttttgtgg aatttaccaa gccaatttac gaccagataa taaataagat tgtcgcctat
      661 taccggaatt tctagaaaag agcgcctaaa tgttggccta cataccgccg gtaagaaatt
      721 ttggcataac aacagtttgt ttgctacctt tgcaccatct aaaagcaatt aatgagtacc
      781 agcacaaata agattcaatt caatacacag tcggactttt accgggaatt aatcagaaat
      841 gtaaacgatt atttcaaagc caataatatt tccaaatatg ggaataggcg catgataatt
      901 aagacaatct gtatgtttgt cttttacttc gctccctttt cgttgctttt cctggatttt
      961 gggtttggac tgtggtctgt tttggtgtta tatgctttaa tgggaattgg caaatcagga
     1021 ataggtcttt ctgtaatgca cgatgcaaat cataattcat tcagcaaaaa tcaaaaagtt
     1081 aataaatgga tgagctactc catgaatatt gtcggaggta gttccattaa ctggaaaata
     1141 cagcataatg ttcttcatca tacttttact aacgttcatg gtcacgatga agacattaac
     1201 gcacctttca ttctaagatt ctcgcctcat caaaagtatt accttattca taagttccag
     1261 tttatttact cctggttctt ctacggtttt ctaacccttg gctggataat gtaccaggat
     1321 tatggccagc ttatcgggta taataaacgg ggactactga aatcgcaaaa gacaactttc
     1381 agcaaggaat tcacaatact tattctgacc aaaatttctt atctggctta tattattgca
     1441 ttgcctatga ttttcggccc tgcagtttgg tggtcttacc tagtaggatt tgccattatg
     1501 cattatgtag ccgggtttat actttccatt attttccaac ctgcccatgt aattgaagag
     1561 actgaatttc ctttaactga tgaatccggc aaacttgcaa caggttgggc ggaacatcaa
     1621 ctaatgacca cctgtaattt tgccagaaac agccggatat tctcctggtt tgtgggtggt
     1681 ttgaattttc aggttgaaca tcacctgttt cccaatattt gtcatgttca ttatcccgga
     1741 atatcagcca ttgtaaaagc caccgcggaa aagtttaatt taccttatta ttcccaacgg
     1801 acattccttt cagcattagt tgctcatggc aaaatgcttt accgtttagg taatccgaaa
     1861 ctggcagcct aagcaatcat ataccctggc taatttattt tcacattcat ttgattgaca
     1921 ggtaaaagat tgtttctaaa tttataatcc acatattctt gtcgatcatg aaaaaacaat
     1981 acctgtttct tcttgccatt attattctgg ccgcgtgtaa gccttctatt aaatatgatg
     2041 ttataatccg gaatggaact gtctatgatg gatcaggagg tgatccggtt gtaaccgata
     2101 ttggtattgt gggcgattcg atagttgcat tggaaaattt agataatatc tccgcggcca
     2161 cagaaattga tgccataggg atggcggttg ctccaggctt tatcaacatg ctaagctggg
     2221 caacaacttc actgatagaa gacggaagat cattgggaga tataaagcaa ggagttactc
     2281 tggaagtatt tggggaaggt tggtctgaag gaccgttaaa tgatgctatg aaagaagaga
     2341 tcacaaagga ccagggtgat ataaagtatg acataaaatg gaccacttta ggtgagtata
     2401 tggattacct ggttgaaaag ggagtcagct gcaacattgc ctcttatgta ggggctacta
     2461 cactgagaat tcatgaactc gcttttgaaa atcggtctcc aactcctgag gaactggagc
     2521 ggatgaaaaa tcttgttcgc acggctatgg aagaaggtgc gctgggaatt ggttcttccc
     2581 ttatctatgc gcctgcgttc tacgcggata ctgaagagtt aattgaactt tgtaaggtag
     2641 cttctgaata tggaggaagt tatatctcgc acatgcgaag tgaaggagat tattttcttg
     2701 aagcaataga cgagctaatg agaatttcta aggaagccaa ccttcccgcc gaaatatacc
     2761 atttgaaagc aggcggcaaa gagaactggc ataaaatgga tttagccctc gctaaaattg
     2821 acagcgccag aaatgccggg cttgccatta ccgccgatat gtataattat attgccggtg
     2881 caaccggact tgatgcctcc atgcctccct gggtacagga agggggttat gatgaatggt
     2941 caaaacgtct aaaggatctg gaaataagaa aaaaaattaa aggtgaaata gaaaataagg
     3001 gtgccggttg ggagaactta tattacgccg caggtactcc ggacaacctg ttgctggtag
     3061 gatttaagga agactcactg aagtatttaa ccggtaaaac tcttactgaa gttgcacaac
     3121 ttcgaaacca ggatccggtt gagacgatga tggacctggt tattcaggat ggaagcaggg
     3181 taggtactgt gtattttctg atgaatgaag aaaacctggt gaagcaaatc aaaaaggatt
     3241 atgtaagctt ttgcagtgat gctgaatcag aagccccgga aggtgttttc ctgaaatcaa
     3301 atccacaccc gcgtgcttat ggtaattttg cccggctttt aggcaagtat gttagggaag
     3361 aaaatgtaat tagcttgcaa gaggcaatta gaaggttaac ttccttgccc gcatcaaaca
     3421 tcaaaataaa aagaagggga agccttgctc cgggcttcaa agcggatgta gtgatttttg
     3481 atccgaatga aatccgggat catgctacat ttgagaaacc acaccaatat gcaaccggcg
     3541 taagggatgt cttcgtgaat ggaatccagg ttttatcagg tggggagcat acaggaaatt
     3601 ttccgggccg ggttgttaga gggccgggat ggaaaggtca ataaaagtta atgaattgta
     3661 aaaactgcgc tgtagaattt gaaggaaatt attgccctaa ttgtggtcag aattccggtg
     3721 taatgcgttt tacgtatccc tattttttaa gagaaacctt tttttccagc ctggatattg
     3781 aacatgggtt tacctattct cttaaagctt tgttcttgcg acctggaaat gcgattcgtg
     3841 attatttgca gggaaaaaga ttgagcctga ctgtaccggg caaatattta attctcatgg
     3901 gagccatctc tactttcctg gctgttcagt atgagatttt caaagttccg gagatcagcg
     3961 aaaaattaat gtggcttccg gatgtagccg gattctttaa atttgctgca caatatacca
     4021 ccgtaattaa tattatcacc ataccgatct ttgctttttt cagttggata attttcagaa
     4081 acaaaggata taactatgct gaaaatacta tcctgaatat tttcattacc tcgcaacaat
     4141 tgcttatgct ggtaattctt gttcctttgg taagcctttt tgaaaccatg aactccgagc
     4201 tggtggcctg ctatacaata atcaccatta tctataacgt ctgggtttac ctgcaatttt
     4261 tcaaattaaa aatagtatca ggaacactat tatcactggc ggcaattctc ttgtcttatg
     4321 caggtcagtt cttctttaac tattttatct acacgatttt gccacagcaa tgggtagagt
     4381 atttagcttt cattacactt taaaattatc tgccctctta tacgcttcaa taactttcag
     4441 ttctccgtct ttaccattta ctgaattgcc atgctccatt cccacgactc cccgaaaccc
     4501 tttttgataa atatgctgaa acacgttttg ataattgatt tccccggtac ccggttcttt
     4561 ccggccaggg ttgtcgccta cctgaaagta ggcaatatca tcccatgcca tttccatgtt
     4621 aggaatcaga tttccttcct gtatttgttg gtgatatata tcaaataata ttttgcagga
     4681 tggactatcc actgcttggc aaagcatttt agcctgaggt attttggtga gaaataatcc
     4741 gggatggtta aaccggttga gtggttccag aaccattact aaattgtgcg gctccagaat
     4801 atcgcaagcc cttcgtaatg cattgatgat gttagcattt tgatagtcga gttcaagtcg
     4861 caaatccaaa tgaccgggaa ctacggtcat ccacttggca ttcacccgct ttgccacctc
     4921 cactgatttc tttatatagt caagaaactc ttcaagctta tccggtttgc cacttgccag
     4981 gttgggctca ctccaatata tcttatgcgc tacaaaaacc cccatggtca tgccaagcgt
     5041 cgacatcttt ttggcaattc gctcctgatc ttcaacctgc cggtccatca ttccattatc
     5101 ttccagggca gtaaagccct ggtcggcaat gaattggagc tggtcagtta gttcttctcc
     5161 cgcggactcc ctgaacattc cgaagtgcgg ggcatatttc aaattaaaag aatttttctt
     5221 ttgaaatgaa tcggcgaata atgcattggc aggaatacct gttaccgcca atgttgaaag
     5281 cgccgccaca ttattaaaaa aagttcgcct tttcatagtt ataagacttc agtttgtccg
     5341 ggtgccggaa tgggataaaa tccattttca tcagggagtg tagggggttg agacttccac
     5401 gaccattgtt caggatcatc caccaatctt ttattggagg caagggcttc atcccagttc
     5461 attactttgc cggaataggt tgccatcctt cccataatag cagtcatggt actctttgct
     5521 ccattttcag catcattgat gatcactttg ccctctttaa ttgcggcaaa cagttcatca
     5581 tgttccacct ggtacgggtt tgggtctccc tcacctgcat gttcataaag tatggagttc
     5641 gggtaattct tcagcagggc tttgttgttc gaatttgtat aaacagatcc tttcgtaccc
     5701 tgaaacgttt cagaaacccg gctcatgcaa tctctttgat gccggcactg acttgagatg
     5761 atggctccgc cgggatattc aaattcaaca aagtgatggt caaagatttc tccatactct
     5821 ttaccggtgc gaacttgcct gccacccata ccctgggccc gaacggggta ttctccgata
     5881 aaccaattgg caacgtcaat gttatggatg tgttgttcca aaatatgatc accgcaaatc
     5941 caggtgaaat aataccagtt ataaacctgg tattccatct ccgtcatgcc gggtttcctt
     6001 tcattcaccc aaacccctcc atcattccaa tacacctgac cggatataat gtcgcctatc
     6061 attccgtcat taattctctt tactgcttcc cggtaattac tttgataatg tctttgcaaa
     6121 ccaactacta cgtttaattt ctttgctttt gcaatttcag cattttccaa aacctttctt
     6181 atgccgtaag catctgtggc aaccggtttc tccataaaaa tatgttttcc ttgttgcact
     6241 gcatattcaa aatgccaggg ccggaatccg ggaggcgtcg taagtataac tacatcggcc
     6301 agatcgattg cttttttata actgtcgaat ccaaggaatt tattttcttc cggcaccgcc
     6361 attcttcccg attcaccatg cactttggtg agcgtttcaa aactgccctt gagtcggtct
     6421 tcaaaaacat cagccatagc cacaagttta acattctctt ttgttttcat ggcctggttt
     6481 gccgcacctg tcccgcggcc gccgcagccg atcagggcca ctttgattgt atcatccaca
     6541 gatgtattga aatgcgaagc aatggaactg aaaggatcaa gcattaccgc acctgcggcc
     6601 atagcgcttc ccttcataaa agttctcctt ttaatgggtg aaatcggatt ttcttttttc
     6661 atggtgctgt taattatagt cttcaatagg tttaagccaa tattttatta tttcttctgg
     6721 agtcggctgt acacgcggtc ttacaattcg aaatcctata aagggtgcgt tagtatgcca
     6781 ccacctgctt ttaggtaact gcggatctct aatcttccat ccaggctgag agtcaattct
     6841 atgagtgggt gtaattttac ttcgttcatc catccagctt ccaccacgtg ctacccttgg
     6901 ataaagttcc tgcggaattc tccagaaatc tttagtggat ttattttcat agggtgcata
     6961 agcatccata gtccattcgg aaacattgcc aagaatatca taaagaccaa gagagttagg
     7021 ttttttaagt ccaacagcat ggtagtgcga agaagaattt cctttatgcc aggcgtaatc
     7081 atccaaagaa ttaataaccg taaagtaatc gtggaggact ccgcctttgg cagtgtattc
     7141 ccactccgct tctgtaggca accggtaaaa atctcctgtt aatgcagaca accatttgca
     7201 ataagagagt gctgcatatt gggtaatgtt tacagcaggg aaaccttcct tgcccatgcc
     7261 gaagctcata tcggtgtacg gttgagtggc cctgcttacg ccatcgatgc ctagttcctt
     7321 aagtttttcg tttgaaattg gatctgcatt atttttatcc cttcgaacaa ataattcata
     7381 ctgctgccag gttacttcat ggacagacat ccaaaagtca gacacctcca tcacatgctc
     7441 cggagattct gttggaattg cattgtttcc catggtgaat ttgccccctt gtattgcaac
     7501 aaattgaatg gattcgttgc tatttggaat cgttaattta taggtaacaa agccattgaa
     7561 aatgtatgcc gggagaacaa aaattaacgg aaacagcagg aacttcagga gtttcattat
     7621 tggtaaacaa gatagtatta ttgaggacaa ttagcaacag ctttacagcc tcacttttca
     7681 ctgtttgcca gttatgttat tgcgaggagc ggagctgcga tgtaatcttt gcctgtatcc
     7741 aaacaacatt tgtgcgagac aacttttgac ttttgtctta tgacttttca cttatgactt
     7801 atgacttatg act
//
LOCUS       DASWHX010000210        19452 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_2096308, whole genome shotgun sequence.
ACCESSION   DASWHX010000210 DASWHX010000000
VERSION     DASWHX010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 19452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19452)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19452
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2096308"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1192
                     /locus_tag="VGA21_13805"
     CDS             <1..1192
                     /locus_tag="VGA21_13805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009578069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX9651632.1"
                     /translation="QPVVEVNISPDVPGEIIELNVEEGDSVTQGQLLVRIRPDNFINA
                     LERAKANYNQQQANLASAKASLARAHANHTRAEADYKRNKELFEQKVLSEAEWDAADQ
                     AYKVANNDLESAKQSVKAAEFILESSKATVGDAEENLRLTNVNAPVYGIVSKLNVEQG
                     ERVVGTQQFSGTEMMRIADLNQMEVLVDVNENDIIRVSIGDTVIIDVDAYSHLDKKFK
                     GLVTSIANTANDKVSQDAVTEFEVKIRILNSSYADLITKGNRFPFRPGMTASVEIITD
                     QKDDILAVPLSAVTTRNKQELERQRLGQNTEDNETVQSTSISPADAELVEIVFVNRDG
                     IAKAIEVKTGISDYENIEILSGLTPGDQIIIGPFLAISRRLKDGDAVKKTEKTAPEPA
                     VATN"
     gene            1268..2275
                     /locus_tag="VGA21_13810"
     CDS             1268..2275
                     /locus_tag="VGA21_13810"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020529525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEX9651633.1"
                     /translation="MSNSFSKILIIQTAFIGDVILATSLIEKLSHYYPEAKIDFLLRK
                     GNQILLKGNPKVGEVLVWNKADKLRETIRIIRLLRKLNYDLLINAQRYFTTGFVSFFS
                     KAKYKIGFDSNPFFFDYDEKYPFKIARNVNEHEVDRLHQLISKITDASPAMPKLYPGN
                     EEEEKILHLIDKPFITIHPGSAWATKQLPVEKWIELVNALNAELQVYLLGGPAEKNLC
                     EKIKVAANKNAVHNKAGELNLLESAALMKCAKVNYVNDSAPLHICSSMDAPCISVFCS
                     TIPEFGFAPLAKHSKVVQIKGDLYCRPCGTHGKLKCPEGHFKCGNSISIEDLNPAEFL
                     Q"
     gene            complement(2279..2848)
                     /locus_tag="VGA21_13815"
     CDS             complement(2279..2848)
                     /locus_tag="VGA21_13815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014798039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family nucleotide pyrophosphatase"
                     /protein_id="HEX9651634.1"
                     /translation="MNLNYTLILGSRSARRQEIMRNAGFEFKVVSIDCEETFPPELDL
                     HNIPKFLAEKKSRAYKTPLEENEMLITADTIVLLNNNVLGKPATINEAKATLTLLSGK
                     SHEVITGVCIRTNVKTVSFHEKTTVSFVELSGNEIDYYIEKQNPIDKAGAYGIQEWIG
                     LIGVESIIGSYYNVVGLPIHKLYSEMKQF"
     gene            complement(2814..4115)
                     /locus_tag="VGA21_13820"
     CDS             complement(2814..4115)
                     /locus_tag="VGA21_13820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013453715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HEX9651635.1"
                     /translation="MAEIRPLRAWRYNPDILPDIDSLTSPLFDVVSIRQREVLYQNPN
                     NSIHISVPLGDNPALTARKTLDSWKKNKIIIQDPIPGIYVYYQYFTLAGEHKELCRKG
                     FIVNIKTYPWDDKVILRHENTMPGSVNDRMQILKEMEFHASPTHGLYTDPGFSLEKFM
                     DESMLTPIYETEDYQGVREVMSVIHDENVINRFVELLKDKQVILADGHHRYEGSLAYK
                     QKCLTNNPNHTGNEAYNYHFMYLTNTELDDLRILPTHRLILGLPNFDEVKFLAKLSED
                     FTIKTIEDAFDVNLIIQGKKAAFGLLISDNAYKLRLKEGKVSELKWNFPDEIKNLDLT
                     ILHYFIIEKALGIPGKDQAASKYIQFDKSFPDCLTRVINKQAQMAIITQDIAIDDVKK
                     VCYSGYTLPPKATYFFPKVICGFLFSSIKENEFELYPDSWI"
     gene            complement(4108..5601)
                     /gene="accC"
                     /locus_tag="VGA21_13825"
     CDS             complement(4108..5601)
                     /gene="accC"
                     /locus_tag="VGA21_13825"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012928064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEX9651636.1"
                     /translation="MKRSITKILVANRGEIALRIIRSAKEMGIKTVAVYSEADRDSLH
                     VRHADEAICIGPPPSKESYLRTDKIINAAIKTGANAIHPGYGFLSENPEFAKKVESGK
                     IIWIGPTSKSIHIMGNKLAAKKAVSKYDIPLIPGSEGAISDKKHARQVAKKIGFPVLI
                     KASAGGGGKGMRVVENDKAFDEQLDRAQSEALSAFGDGSVFIEKFVNSPRHIEFQVMG
                     DQYGSIIHLFERECSIQRRHQKVIEESPSSVLTPELRERMGKAAVDVARSVNYVGAGT
                     VEFILDDKMNFYFLEMNTRLQVEHPVTEMITGLDLVKLQIQIAQGEKLKLKQSDVQIR
                     GHAIESRVYAEDPENQFLPDIGTLKTYITPKGIGTRVDDAYEQGMEIPIHYDPMIAKL
                     VTYGSDRKEAIARMVRAIDEYIITGVATTLSFCKFVMNHKAFVTGKFDTKFVENYYQP
                     ENRKLDKDELRAAAALLVYLKEKDKIKTAGNQNNNAKGWLNRLKDNG"
     gene            5765..7015
                     /locus_tag="VGA21_13830"
     CDS             5765..7015
                     /locus_tag="VGA21_13830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009578080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HEX9651637.1"
                     /translation="MDLFSKLRVEEGLLGQHSQIKPDYFMFPKLEGEIKPRMKFEGRE
                     MIIWSLNNYLGLANHPEVRRVDAEAAAKYGLAYPMGARMMSGNSVQHKELEKQLADFV
                     SKESALLLNYGYQGVISIIDSLVDRRDVIVYDAESHACIVDGVRLHMGKRFVYNHNDM
                     ESLEKQLGHATKLVDETGGGILVITEGIFGMSGNMGKLKEIIALKQKYEFRLFVDDAH
                     GFGTMGATGAGAGEEQGVQDGIDLYFSTFAKSMASIGAFVAGNTSVTEHLRYSLRSQI
                     FAKTLPIALVIGALKRLELIRSQPQLRENLWKITKALQTGLQEQGFNIGKTQSPVTPV
                     FFFGGVGEAANMAYDIRERYNIFCSMVVYPVVPKDVIIFRLIPTAVHTLDEVEETIDV
                     FSKTKEKLASGSYKRQELIVINTR"
     gene            7122..7295
                     /locus_tag="VGA21_13835"
     CDS             7122..7295
                     /locus_tag="VGA21_13835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010856743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone H1"
                     /protein_id="HEX9651638.1"
                     /translation="MNRFEELKNLVMSLEGDFSKFYEKKNQAAGTRVRKGMQDLKNLA
                     QDIRKEVQGIKNE"
     gene            complement(7340..8209)
                     /gene="dapA"
                     /locus_tag="VGA21_13840"
     CDS             complement(7340..8209)
                     /gene="dapA"
                     /locus_tag="VGA21_13840"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009195475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEX9651639.1"
                     /translation="MFKGTGVALVTPFNPDFSIDFDGFSRLLEHTAKGVDYYVVQGTT
                     GESVTTSNEEKTQLLEFVKKNNPRNLPIVFGIGGNNTQIILSSIKQTDFEGVSGLLSV
                     SPYYSKPSQEGIVQHFIRIADTSPVPVILYNVPGRTSSNLTAETTLRLADHANIIGIK
                     EASGDLNQCQIISRNKPKEFLLISGDDLLTVPILSIGGVGVISVLANAYPEIFRQICR
                     NFETGNLVESRLESFKLLEINPLMYAEGNPVGVKEVLKKMGVCGNEVRLPLVTASETL
                     RERIYLAMKNIRA"
     gene            complement(8202..8735)
                     /locus_tag="VGA21_13845"
     CDS             complement(8202..8735)
                     /locus_tag="VGA21_13845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651640.1"
                     /translation="MFSKTLLNYRTSRRLKQNTLERDSIGFSKSRLIGILFTMESEEK
                     FSKIKMLQKDFEQLGKQVEVLSFLPKGATNYEFLFNIFTLQDISFTGKFRNEDVNKFA
                     ATRFDYLLVFDSSLSPIMRNIVARSNAHCRMGCYFEDSEKLFEFMISQNEQRNSLHLI
                     NEITKYLKGLNKESAYV"
     gene            complement(8739..10748)
                     /gene="ligA"
                     /locus_tag="VGA21_13850"
     CDS             complement(8739..10748)
                     /gene="ligA"
                     /locus_tag="VGA21_13850"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011584700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEX9651641.1"
                     /translation="MTKDEAKRKIADLTEQINYHNRLYYLEDRIKIEDFEFDKLLQKL
                     IDLENKFPEFRYPDSPSQRVGGAITKEFKTVEHKFPMLSLGNTYSKEDLDEFDARVCK
                     GLNKSEVEYFCELKFDGVAISVTYENGMMVRAVTRGDGTKGDEITQNARTIRSLPLKV
                     PGKVPEMFEVRGEVFLSKENFKKLNEFRMEAGEELYANARNTASGTLKMQDSALVAVR
                     KLDCFLYSVMGENLNLKSHSDAIDFLIKHDFNVSKTFKRCKSLEEVLHYINIWEQKRH
                     TLPLETDGVVVKVNDIKDQIKLGFTAKNPRWAIAYKYKAEAAVTRLQGITYQVGRTGA
                     ITPVAELEPVFLAGTTVKRASLHNANEIKRLGLHIGDYVHVEKGGEIIPKITGVELSK
                     RKKSEQIQYISRCPECNTKLVRIEGEALHYCPNHKGCPPQIKGRIEHFIGRNTMDIDS
                     LGSRTIGLLFEKGLLKQPSDLYHLNYQDIFYLEGFKDQSTKNILKGIEESKKTGFAKV
                     LFALGIRHVGKTVAEKLAHHFKSIEAIKNASLEELLEAPEIGEVIAQSLVDFFFDNEN
                     LKEIERLKKAGLNFELKEEENRLEGDTLVGKSFVISGVFENFGRDEIKETIKRNGGKL
                     LSSISGNVDFLVAGQNMGPAKKKKASDLGVRIITEEEFIAMLNHT"
     gene            complement(10769..12646)
                     /locus_tag="VGA21_13855"
     CDS             complement(10769..12646)
                     /locus_tag="VGA21_13855"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009579965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEX9651642.1"
                     /translation="MKKLLIVTLILIPSLANSQPDRWQQQVDYTMEIDLDDITHQFKG
                     IQKIIYTNNSPDTLTHIYYHLYFNAFKPGSMMDIRSRTIKDPDNRVRERIFNLKTNEI
                     GFLKVRSLKDETGKNLDHKTEKSDETILEVALHTPILPNTTTVFSMEFDGQVPLQIRR
                     SGRDNAEGISYSMAQWYPKLAEYDYEGWHTNPYIGREFYGVWGNFDVTLKLDAKYVVA
                     ATGVLQNPEETGHGYEKSELKVKRPKGKLEWHFKADNVHDFVWAADPDYIHDVVQTDF
                     GLELHFFYQNDTSIIKNWKQLPAYTEKAFRFMNENFGKYPYSEFSVIQGGDGGMEYPM
                     ATLIRGNGNLRGLVGVTLHELIHSWYYGVLGTNESLYPWMDEGFTSFASSVVSNYLFQ
                     RDVEANPVARQHSAYFSLVESGKEEPMTTHSDHFNENRSYSTSAYSKGATALGQLNYI
                     MGREVFMKAMRRYYNTWKFRHPNLTDFKRVMEKESGLELDWFFEYFIYSTKTIDYAIR
                     TVVAPTNMETEIRIDRIGHMPMPLDILVEYKDGSQEFYYIPMVLMRGEKKPEETTVPR
                     TVLDDWAWTDPVYTFLVATNGRQIQSVEIDPSQRMADIDRSNNRIEINYKMSETIENE
                     K"
     gene            complement(12735..12941)
                     /gene="xseB"
                     /locus_tag="VGA21_13860"
     CDS             complement(12735..12941)
                     /gene="xseB"
                     /locus_tag="VGA21_13860"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009579964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEX9651643.1"
                     /translation="MAKSNKFKYTEAVREIEEILNEIEAGELEVDILAERVRRAGELI
                     KLCREKLRKTEEDLEENLNEEEFD"
     gene            complement(12919..14175)
                     /gene="xseA"
                     /locus_tag="VGA21_13865"
     CDS             complement(12919..14175)
                     /gene="xseA"
                     /locus_tag="VGA21_13865"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009579963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEX9651644.1"
                     /translation="MNHHSLFELNSLVKNVLSQHLQPSYWVVAEIGEMHLNQKGHCYL
                     ELIEQENDEVIAKAKATIWAYTYRNLSAWFESITQQSIQTGLKLLMNVQVQFHEVFGF
                     SLNIRDIDPTYTLGESEKKKTAVLEKLTKEGIIDMNRELMLPVVPQRIAIISSPTAAG
                     YEDFIDQLEKNPDNFFIQTHLFKAIMQGQEALFSIPEALRRIHQRMNDFDLVVIIRGG
                     GSQVDLDCYDSYDICSHIAQFPIPVITGIGHERDESIADRVAHTRLKTPTATAEFLLI
                     GFREFENELLSLRQRIHRVAETRIENERLDLDAFANSIRLLSRGLIQSNVTKLNFFET
                     QMIKLPVSFLSINRTALKNYDERLRLLDPKSILKRGFTISTINNVLLKNVKGISMGDL
                     LVTQTHDSFIESDIKNIYQDGEKQQI"
     gene            complement(14231..14683)
                     /locus_tag="VGA21_13870"
     CDS             complement(14231..14683)
                     /locus_tag="VGA21_13870"
                     /inference="COORDINATES: protein motif:HMM:NF019430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HEX9651645.1"
                     /translation="MKKISIIVAVLFGMQPAFAQEPEAKMRAARIGFLTNRVELTPQQ
                     AEKFWPLYNQFNDKRENVQIEKRMMHMEMNKNGLTDDEAKKMIEKELKTRQDELNLEK
                     EFIADIQTVLTPVQAIKLLRAERDFNMEVLRNLQQRRNQPNHEGRPGN"
     gene            complement(14694..15116)
                     /locus_tag="VGA21_13875"
     CDS             complement(14694..15116)
                     /locus_tag="VGA21_13875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651646.1"
                     /translation="MKKLEQIPKKEVFQVPEGYFEELPAQIRKKIDADKSPIWYGRPQ
                     FRIALASITIILAIIFSVVHFSATELLIQEELSEISDERLINYLESADISLVDIIEEI
                     SIDEDTYLEIMKENFTFDSDKIEEEILLNLLKYSDELL"
     gene            complement(15149..15688)
                     /locus_tag="VGA21_13880"
     CDS             complement(15149..15688)
                     /locus_tag="VGA21_13880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019986937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX9651647.1"
                     /translation="MEEKDILDKLLNPETRDYGFNLLVTHFQERVYLLARKMVIDHDD
                     ANDITQDVFIKVYQNLEKFRGDSSLFTWIYRITSNECISFLRKKKIRNFLPWSQSSDN
                     WKSQIDNSPLISGDEIQKKLQKAILTLPEKQRLVFNLKYFDELKYEEIAEITGTSVGG
                     LKASYHLAVKKIEQFLNDH"
     gene            complement(15721..15873)
                     /locus_tag="VGA21_13885"
     CDS             complement(15721..15873)
                     /locus_tag="VGA21_13885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651648.1"
                     /translation="MKKLIERIFDKLPGTNNDLTYFQKVWVIAIAFAFSLIILIKAIT
                     HIINNF"
     gene            complement(15873..16820)
                     /locus_tag="VGA21_13890"
     CDS             complement(15873..16820)
                     /locus_tag="VGA21_13890"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX9651649.1"
                     /translation="MKPFRNILLGLDASDLDPLLIKFASFMSRSNPTRAFYFVNIIKH
                     MKVPKEVRKEFPEIVEAALKDRKSHINDSIRENLVMHPDSTSKVIVKNGDPAKEILKL
                     IEQLDIDLIVMGKKPSAESSGVVLHQVARRADCNLLIVPKGGDAVLNDLRTSRKLLVA
                     NDYSNHSELAMEHAVNMALNSDTKKTIEIYCQHVYTVPTGYHYTGKTYEEFAEIMRNN
                     AEKSYKKFIKKIDIKGSKIIPVYSLETHGHLVSSIYRMAVEINADVIIFGARGVSSTT
                     AIFIGSFAEKMLKIDSTIPLLVVRTKGDNVGFLEYIRKF"
     gene            complement(17128..19014)
                     /gene="serA"
                     /locus_tag="VGA21_13895"
     CDS             complement(17128..19014)
                     /gene="serA"
                     /locus_tag="VGA21_13895"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009183497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HEX9651650.1"
                     /translation="MSEKYFIIDFDSTFTQVEALDILAGISLKGNKRKAALKEIKSIT
                     EKGMHGEINFKKSLTERIRLLEANKKHLPPLIKKLKEKVSPSFKRNREFLTKYRNHIF
                     IISNGFREFIVPIVSEYGIRDDHVFANSFQFDKDGGIVGFDKKNVLSNNNGKVKQLAS
                     LKLSGEVYVIGDGYTDYEIKAAGLSNKFFAFTENVERKHILEQADHVAPSLDEFLFVN
                     KMNGSLSYPKNRIKVLLLENVHSKAFDLLKKEGYQVEEINESLSEEELLKKIPDISIL
                     GIRSQTHVTKRIIEQARRLISIGAYCIGTNQIDLDECTKKGIVVFNAPFSNTRSVVEL
                     AIAHIIYLMRRITAVNADMHKGNWNKSAVGSHEIRGKKLGIIGYGNIGTQLSVIAESL
                     GLDVYYYDIVEKLALGNATKCNSLKELLNLADIVSLHVDGRKENKNLISSTQLKWMKK
                     NAILINLSRGHVIDLDDLKKVLQSNKLGGAAIDVFPEEPLGRNKKFKSPLLELPNVIL
                     TPHIGGSTEEAQENIANFVPNKIMDYVNTGKTTNSVNFPNLNLPPLENAHRLIHIHQN
                     TSGVLASINQVLAAHNINILGQYLKTNELIGYVITDIDKEYNREVVQELKKIDGTIKF
                     RVLY"
     gene            complement(19165..>19452)
                     /locus_tag="VGA21_13900"
     CDS             complement(19165..>19452)
                     /locus_tag="VGA21_13900"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEX9651651.1"
                     /translation="GNLVTSIEDKLSREGISVYPNPADRSFTIGFKSPVTEELTIRVF
                     DTFGKVMMTSIVPAGSTSINVDAKRLKNGMYVVQIDHENLVITRQKIVVVH"
BASE COUNT         5503 a         3971 c         3702 g         6276 t
ORIGIN      
        1 tcagccggtg gtagaagtca atattagccc ggatgttccg ggggagataa ttgaacttaa
       61 tgttgaggag ggcgattctg taactcaagg tcagcttttg gtgaggattc gacccgataa
      121 tttcataaat gcattggaaa gagctaaggc aaactataat caacagcagg caaatcttgc
      181 ctccgcaaag gcaagccttg ccagagcgca tgcaaatcac acccgggctg aggcagatta
      241 taagcgcaac aaggaattgt tcgagcagaa agttctatct gaagctgagt gggacgcggc
      301 tgatcaagcc tataaagtcg ctaataatga tttagagtcc gcgaagcaat ctgttaaagc
      361 agcggaattc attttggaaa gctcaaaggc aacagtagga gatgcagaag aaaatctaag
      421 attaactaat gttaatgctc ctgtatatgg aattgtatcc aaactaaatg ttgaacaggg
      481 cgaacgtgtt gtaggtactc agcagttctc aggaacggag atgatgcgga ttgctgacct
      541 gaaccagatg gaagtgttag ttgatgtaaa tgaaaacgat attatcaggg ttagtattgg
      601 cgatacggtt attattgatg ttgatgcata ctcacacctg gataaaaaat ttaaaggact
      661 ggtcacttcc atagccaaca ccgccaatga taaggtttcc caggatgctg taactgaatt
      721 tgaagtaaag atcagaatac ttaattcatc ttacgcggat ttgattacca aaggaaacag
      781 gtttccattc agacccggta tgactgcgag tgtcgaaata attacagacc aaaaggatga
      841 cattttggct gttcctcttt cagcagttac cacaaggaac aagcaggaat tagagcggca
      901 acgtttagga cagaatacgg aggacaacga aaccgttcag tcaacttcta tttctccggc
      961 agatgctgaa ctggttgaaa ttgtttttgt aaaccgtgat gggattgcga aagcgattga
     1021 agttaaaacc ggaatcagtg attatgagaa tatagagata ctaagcggat taactccggg
     1081 agaccaaatt attatcggtc cttttctggc catttcccgc cggctgaaag acggtgatgc
     1141 ggttaaaaag acagaaaaga ccgcacctga accggcggtt gcaacaaact aagcagtcaa
     1201 ttaactaaaa aatgtggaag cccttgtcca aagcgagggc ttttttatat tttagaagca
     1261 aggcaaaatg agcaattcgt tttcaaaaat cttaattatt caaaccgctt tcattgggga
     1321 tgtgattctt gctacatctt tgattgaaaa attatcgcat tattaccctg aagccaaaat
     1381 agacttcctc ttgaggaagg gtaaccaaat cttgcttaag ggaaacccaa aggtaggtga
     1441 ggttttggta tggaataaag cagataaatt aagggaaacg attagaataa ttaggttatt
     1501 aagaaaattg aattatgatc ttttgattaa tgcccaacgc tattttacta ccggctttgt
     1561 aagttttttt tcgaaagcta aatacaaaat tggtttcgat tccaatcctt ttttctttga
     1621 ttatgacgaa aaatatccat tcaaaatagc gcgcaatgtg aacgaacatg aggttgacag
     1681 gttacatcaa ttgatttcca aaataactga tgccagtccg gcgatgccta aattgtatcc
     1741 cggtaacgaa gaggaagaaa aaattcttca tttaatagat aaacctttta ttacaatcca
     1801 tcccggatcg gcttgggcga ccaaacaatt acctgttgaa aaatggattg aattggtaaa
     1861 tgcacttaac gcggaattac aagtttatct tttaggtggt cctgctgaaa agaatttgtg
     1921 tgaaaaaatt aaagtagccg caaataaaaa tgcagttcat aataaggcag gcgaactgaa
     1981 tcttttggaa tcggcagcct tgatgaaatg tgccaaagtg aattacgtga atgattcagc
     2041 cccgcttcat atttgttctt ccatggatgc accttgtatt tcagtttttt gctctaccat
     2101 tcctgagttt ggttttgctc ccctggcaaa acattcaaaa gttgttcaaa taaaaggaga
     2161 tttatattgc aggccatgtg gaactcacgg gaaattaaaa tgtcctgagg gacacttcaa
     2221 atgtggcaat agtatttcaa ttgaagactt gaatccggcg gaatttcttc aataaaacct
     2281 agaattgttt catttcacta taaagcttat ggatgggtaa tcctactaca ttatagtatg
     2341 aaccaataat agattccacc ccgatcaaac caatccattc ctgtatgccg taagctccgg
     2401 ctttatcaat tggattttgc ttttcaatat aataatcgat ttcattgccg gaaagttcaa
     2461 caaatgaaac agtggttttt tcatgaaatg aaacagtttt gacattcgtc cggatgcaaa
     2521 ccccggtaat tacttcatga gattttcctg aaagtaaagt cagggttgct tttgcttcat
     2581 ttatggtggc aggtttccca agcacattat tatttaataa aacaatggta tctgctgtga
     2641 ttagcatttc attttcttcc aaaggtgttt tatatgctct ggattttttt tcagcaagaa
     2701 acttgggtat gttgtgcaaa tccagttcgg gagggaaggt ctcttcgcaa tcaatggaaa
     2761 ccaccttgaa ttcaaagccg gcattacgca ttatttcttg tcttcgcgca gacctagatc
     2821 caagaatcag ggtataattc aaattcattt tcttttattg agctaaacag gaatccgcag
     2881 ataacctttg gaaagaaata tgttgctttg gggggtaggg tgtaacctga gtaacaaact
     2941 tttttcacat catcgatggc aatgtcctgg gtaataatgg ccatttgtgc ctgtttattg
     3001 attaccctgg taagacagtc cggaaaactt ttgtcaaatt ggatatactt tgaagcagct
     3061 tgatctttgc ctggtattcc caaagctttt tctataatga agtaatgcaa aattgtaaga
     3121 tccaggtttt ttatttcatc aggaaaattc catttcaatt ccgaaacctt gccttctttc
     3181 agcctgagtt tatatgcatt gtcgctgatc aataatccaa aagcggcttt tttaccctga
     3241 atgatcagat taacatcaaa tgcgtcctca attgtcttaa ttgtaaagtc ctctgacaat
     3301 tttgccagaa atttaacttc atcaaagttt ggcaacccca aaattaaacg atgtgttggc
     3361 aagattctta aatcatcaag ctcggtattt gtaagataca tgaagtggta attgtacgct
     3421 tcgtttccag tatgatttgg attattcgtc aagcattttt gcttgtaagc taatgagcct
     3481 tcatacctgt ggtgaccgtc ggcaagtatt acctgcttat ctttaagtaa ctctacaaac
     3541 cgattgatta cattttcgtc gtgaatgaca ctcatcacct cccggacacc ctggtaatct
     3601 tccgtttcat aaataggagt caacatactt tcatccataa atttctccaa tgaaaaccca
     3661 ggatcagtat acaggccatg ggtcgggctt gcatgaaact ccatttcctt caggatttgc
     3721 atcctgtcgt ttacagagcc aggcatcgta ttttcgtgtc gaagaattac tttatcatcc
     3781 caaggatagg ttttaatgtt cactatgaat ccttttctgc agagttcttt atgctcaccg
     3841 gcaagggtaa aatattggta atacacgtaa attccaggta ttggatcctg aataataatt
     3901 ttatttttct tccaggaatc aagagtcttt cgtgcggtta aggccggatt atccccaaga
     3961 ggtactgaaa tgtgtatgct attattaggg ttttgatata aaacctctct ttggcgtatt
     4021 gaaacaacat caaaaagggg agaggttaga gaatcaatat cagggagaat atccggatta
     4081 tatcgccagg cacgcagcgg tcttatttca gccattgtct tttaatctat tgagccaacc
     4141 ttttgcattg ttgttttgat tgccggccgt tttaattttg tccttctcct taagatatac
     4201 caataatgct gccgcggctc ttaactcatc cttatccaac tttctgtttt ccggttggta
     4261 ataattttca acaaactttg tgtcaaattt tccggtaaca aaggctttat gattcattac
     4321 aaatttgcaa aagcttaaag ttgtggcaac accggtaata atatactcgt caatcgcgcg
     4381 gaccatcctt gcaatagctt ccttccggtc agaaccataa gttaccagtt tagcaatcat
     4441 tgggtcgtaa tgtattggta tttccatgcc ttgctcatag gcatcgtcta cccgggtgcc
     4501 aattcctttc ggcgtaatgt aggttttcag ggttccaata tccggcaaaa attgattttc
     4561 aggatcttca gcataaactc tggactcaat agcgtgaccc cttatctgca catcagactg
     4621 ctttaatttt aatttttcac cctgggcaat ttgaatttgt aatttcacca aatcaagacc
     4681 tgttatcatc tcggtaacgg gatgctctac ttgtaacctg gtattcattt ccagaaaata
     4741 aaaattcatt ttatcatcta aaataaactc gacagtacct gcacctacat aatttactga
     4801 tcgggcgaca tcaactgcag ctttacccat cctctccctg agctccggag ttaaaacgct
     4861 cgacggtgat tcctcaataa ccttctgatg ccgcctttga atagagcatt ctctttcaaa
     4921 caaatgaata attgaaccat attgatctcc catcacctgg aattcaatat gtctcggaga
     4981 gttcacaaac ttttcaataa agaccgagcc atctccaaaa gcactcaagg cttcactttg
     5041 tgcccggtcc aattgctcat caaaagcctt gtcattttca actacacgca taccctttcc
     5101 acctccaccg gcactagctt tgatcaggac tggaaaccct attttttttg caacctggcg
     5161 ggcatgtttc ttatctgaaa ttgctccttc gcttcccgga attaatggaa tatcatactt
     5221 gctcacggct ttttttgccg caagtttatt tcccattata tggatgctct tcgaggtcgg
     5281 gccaatccag ataatttttc cactttccac tttctttgca aattccggat tctccgaaag
     5341 gaatccataa ccgggatgga ttgcatttgc accggttttt attgctgcat ttattatttt
     5401 atcagttcta agataactct cttttgacgg tggagggcca atacaaatgg cttcatctgc
     5461 atgccgtaca tgaagagaat ccctgtctgc ttctgagtaa acagccaccg ttttgattcc
     5521 catttccttt gccgaacgaa taattcggag tgcaatctca cctctgtttg ctacaagtat
     5581 tttagtaatg ctccttttca aaatccgact tttttaaagt tacaatgcta aataattatt
     5641 atctgaatgt ctacttatgg ccttcattaa ttgaattgag ggtcttttga gttgaatttt
     5701 ttaattatta gctttgtgag gccttttagg ctaatcacta atcaaccatc aaaatcttaa
     5761 aatagtggat ttattctcaa aactgcgagt agaggagggg ttgctggggc aacattctca
     5821 aattaagccg gattacttca tgtttcccaa gttagaaggt gaaataaaac ctcgaatgaa
     5881 atttgaaggg cgtgaaatga tcatctggtc attgaataat tacctgggac tagccaacca
     5941 tcctgaagta agaagagttg atgctgaagc cgcagcaaaa tatggacttg cttatccaat
     6001 gggtgccaga atgatgtctg gtaattcggt ccaacataaa gaattagaaa agcaattagc
     6061 tgactttgtc agtaaagaat ccgcactcct gctcaactat ggataccagg gagtaatttc
     6121 tataattgat tctctggttg ataggagaga tgttatcgtg tatgatgcgg aatctcatgc
     6181 atgtattgta gacggagtga gacttcatat gggtaaacga ttcgtgtata accataatga
     6241 tatggagagt ctggaaaagc aacttggaca tgccacgaaa ttggttgatg aaaccggtgg
     6301 cggaattctg gtaattacag aagggatttt tgggatgtct gggaatatgg gaaaactaaa
     6361 agagattatc gctcttaaac agaagtacga attcagatta tttgtggatg atgcgcacgg
     6421 ttttggaaca atgggtgcca ccggtgcggg agcaggtgag gagcagggcg ttcaagatgg
     6481 cattgatctc tatttttcaa ccttcgccaa atcaatggct tctatcggtg cttttgttgc
     6541 tggaaataca agcgtgaccg agcatttacg gtactcgttg agatctcaga tttttgccaa
     6601 aacattaccc attgcacttg taattggtgc tctgaaacga ttagaactta tccggtcaca
     6661 gccgcagttg cgggagaatc tctggaaaat aaccaaagcg ctccaaaccg ggcttcagga
     6721 gcaagggttc aacataggga aaacccagtc tcctgtaact ccagttttct tttttggtgg
     6781 cgttggcgaa gctgcaaaca tggcttatga tattagagaa aggtataaca ttttctgtag
     6841 catggttgtt tatcctgttg tgcctaaaga tgtgattatc tttaggttaa tcccgacagc
     6901 cgtacataca cttgacgagg ttgaagagac catagatgta ttttcaaaga caaaagaaaa
     6961 acttgcaagt ggcagctata agcgacaaga acttattgtc ataaataccc gctaaatgcc
     7021 tttaattggc taaaaaaggc aagtttttaa tagtttttta atcattttga ctatattgcc
     7081 tcagaatcta aatttatcaa taaccttaaa tatagcttca catgaataga tttgaagaac
     7141 taaaaaatct tgtaatgtca ttagagggtg acttctctaa attctatgaa aagaaaaatc
     7201 aagctgccgg aactcgagtt agaaagggta tgcaagactt gaagaacctt gctcaggata
     7261 ttcgaaaaga agttcaaggc atcaagaatg agtagtaatg cttaaaaaca ttaagaaaag
     7321 ggctcaaatg gccctttttt tatgccctga tgtttttcat ggccaggtag attctctctc
     7381 ttagagtttc ggatgcagtt accaggggta atcttacctc attcccacaa acccccattt
     7441 tcttcaacac ttcttttacc cctaccggat tcccctcagc gtacattaag ggattgattt
     7501 ccagtagttt aaatgactcc aaccggcttt caaccagatt gccggtttca aaattccggc
     7561 aaatttgcct gaagatttcc gggtaggcat tggccaatac ggaaattacg ccaacgccgc
     7621 cgatagaaag tatgggcact gtgagcaaat catctccgga aataagcaga aactctttag
     7681 gtttattcct ggaaatgatt tggcattggt tcaaatctcc tgaagcttct ttaataccta
     7741 ttatgtttgc atgatccgct aaccgaagcg tagtttccgc agtcagatta cttgaggtgc
     7801 gcccgggcac attatagagt ataacaggta caggtgaggt atctgcaatt ctaatgaagt
     7861 gttgaacaat tccttcctga cttggtttgc tataataagg acttacagaa agcaatcctg
     7921 aaacaccttc aaaatctgtc tgcttaattg aacttaggat aatttgcgtg ttatttccac
     7981 ctatgccaaa cacaatggga agattgcgcg ggttattctt tttcacaaac tcgagaagtt
     8041 gtgttttctc ctcgttgctg gtagttaccg attctcctgt agttccctga accacatagt
     8101 agtctacacc tttggcagtg tgttcgagca gtcggctaaa cccatcaaaa tcaattgaaa
     8161 agtcagggtt gaagggtgtg acaagagcta ctccggttcc tttaaacata cgcgctttcc
     8221 ttatttaatc ctttcaggta tttagttatc tcatttatta aatgtagtga gtttctttgc
     8281 tcgttttgag aaatcataaa ttcgaataat ttctcgctat cttcaaaata acagcccatt
     8341 ctgcagtgcg cattactcct tgccacaata tttcgcatga tgggagacag ggagctgtca
     8401 aacactaata aataatcgaa tcttgtagcc gcaaatttgt ttacatcttc atttctaaac
     8461 tttccggtaa atgaaatatc ctgaagggta aaaatattga aaaggaattc ataattagtg
     8521 gcacctttgg gaaggaaact cagaacttca acctgcttac ccaattgctc aaagtccttt
     8581 tgcagcattt ttatcttgct gaatttttct tctgattcca tggtgaacag gattcctata
     8641 agcctggatt tactaaaacc gatagaatcc cgctccaggg tattttgttt taggcgccgg
     8701 gaagtcctgt agtttaatag ggtcttgctg aacatcgatt atgtatggtt aagcatcgca
     8761 ataaattcct cctcagtaat tattcgaaca cccaggtccg atgctttttt cttttttgcc
     8821 gggcccatgt tttgccctgc aaccagaaaa tcaacattac cggagatgga tgaaagtaat
     8881 ttgcccccat tcctttttat cgtctcttta atctcgtctc ttccaaaatt ttcaaacact
     8941 cccgaaatta caaaagattt tccaaccagc gtatctcctt ccagccgatt ttcctcctcc
     9001 ttaagttcaa aattgagccc ggcttttttt agcctttcta tttccttaag attttcattg
     9061 tcgaaaaaga agtccaccag actttgtgcg atgacttctc ctatttccgg cgcttccaat
     9121 aactcttcca aagaagcatt cttgatcgcc tcaatacttt tgaaatgatg ggctaacttt
     9181 tccgctaccg ttttcccgac atgccggata ccgagggcaa ataatacttt tgcaaaaccc
     9241 gttttctttg actcttcaat ccccttcaga atattttttg tcgactggtc tttaaaacct
     9301 tcaaggtaaa aaatgtcctg ataattaagg tggtatagat cagaaggctg ctttagaagt
     9361 cctttttcaa aaagtaaacc aatggtgcgg cttccgaggc tgtctatgtc catcgtattt
     9421 ctacctatga agtgttcgat tcttccttta atttgcggag gacacccttt atggttagga
     9481 cagtaatgaa gggcttcccc ttcgatcctt accagtttag tattgcactc aggacaccgg
     9541 gaaatatatt gaatttgttc cgatttttta cgcttcgaca attcaacacc ggttattttt
     9601 gggatgattt cacctccctt ttcaacatga acataatctc caatgtggag tccaagcctt
     9661 tttatttcat tggcattatg caacgaggct cgctttacag tggtgccggc caggaaaaca
     9721 ggttctaatt ctgctaccgg tgtaattgcc ccggtccggc caacctgata agtaattccc
     9781 tggagccggg ttactgcagc ctctgctttg tatttatacg cgatggccca gcgaggattt
     9841 tttgcagtga agcccagttt gatctgatct tttatgtcgt taacttttac tacgacgcca
     9901 tcggtttcaa ggggaagcgt atgccttttt tgctcccaaa tgttaatata atgcagcact
     9961 tcctccaggc ttttacacct tttgaaggtt ttggaaacat tgaaatcgtg tttgataagg
    10021 aagtcaatgg catcggaatg gctcttcaaa ttcagatttt ctcccattac tgagtacaaa
    10081 aaacaatcga gctttcttac tgctaccaat gcactatctt gcattttaag ggttccgctg
    10141 gcggtgttcc ttgcattcgc gtaaagctct tcacctgctt ccatcctgaa ctcattcagt
    10201 ttcttaaaat tctcctttga tagaaaaact tcacccctta cttcaaacat ttcaggcact
    10261 ttaccgggaa cttttaaagg cagacttcgg attgttcgtg cattctgagt tatttcatcg
    10321 ccctttgttc cgtcgccgcg cgtaacggcc cttaccatca tcccgttttc ataagtaaca
    10381 cttatggcta ctccatcaaa ttttaattca cagaaatatt ctacttctga tttattcaat
    10441 cctttacaaa cccgggcatc aaattcatca aggtcttctt ttgaataggt atttcccagt
    10501 gagagcattg ggaatttatg ctccacagtt ttgaactctt tggtaatagc tccaccaact
    10561 ctttggcttg gagaatcggg atatcgaaat tcggggaact tattctcaag atcgattaat
    10621 ttctgtaaga gtttatcgaa ctcgaaatct tcaattttaa ttcgatcttc cagatagtac
    10681 agccggttgt gataatttat ttgctcagtt aaatctgcaa tctttctctt tgcttcatct
    10741 ttggtcatag acaaaacatc taagtatgct atttttcatt ttcaatggtc tcactcatct
    10801 tataatttat ttcaatccgg ttgtttgatc tgtcaatgtc tgccattcgt tgagagggat
    10861 caatttctac agattgaatt tgcctgccgt ttgtggcaac taaaaaggta taaactgggt
    10921 ctgtccatgc ccagtcgtcc aaaaccgttc ggggtacagt agtttcttcc ggcttttttt
    10981 cacctcgcat taagaccatt gggatgtaat aaaattcctg tgatccgtct ttgtactcaa
    11041 ctaatatgtc caggggcatt ggcatatgac caatcctgtc aattcgaatt tctgtttcca
    11101 tatttgtagg tgcaacaacc gttctgattg catagtcaat agtttttgta gaatatataa
    11161 aatattcgaa aaaccaatcc agctcaagac cggattcttt ctccataact cgcttaaaat
    11221 cagttagatt ggggtgcctg aatttccagg tattgtaata tcttcgcatg gctttcataa
    11281 agacttccct gcccattata taattgagtt gccccaacgc tgttgcacct ttggaatatg
    11341 cagaagttga atacgatctg ttttcattaa agtggtcgct gtgtgtggtc atgggctcct
    11401 ctttcccgga ctctaccagg gaaaagtatg cagagtgttg cctggctacc ggattggctt
    11461 caacatccct ctgaaacaaa taattcgata cgactgaact cgcaaatgaa gtgaaaccct
    11521 catccatcca ggggtataga ctttcatttg ttccaagtac accataatac cagctgtgaa
    11581 tcaattcatg tagtgtcacg ccaactaatc ctcttaaatt gccattacca cggattaagg
    11641 tagccattgg atattccatt ccaccgtcgc ctccctggat tacagaaaat tcagagtatg
    11701 ggtactttcc aaaattctca ttcataaacc ggaaggcttt ttcagtataa gcgggaagtt
    11761 gcttccagtt ttttatgata gatgtgtcat tctgataaaa aaaatgcaat tccaaaccga
    11821 agtctgtttg gacaacatca tggatataat caggatctgc agcccaaaca aaatcgtgga
    11881 cattatctgc tttgaaatgc cattccaatt tgcctttggg ccgcttcact ttcagttcag
    11941 atttctcata tccatgcccg gtttcctccg gattttgaag aacacctgta gctgcaacaa
    12001 catatttagc atcaagtttt agagtgacat caaagtttcc ccagactcca taaaactccc
    12061 tgccgatgta tggattggtg tgccagccct cataatcata ctctgccagt ttcggatacc
    12121 actgagccat ggaataggaa attccttccg cgttatctct gccagatctt cggatttgca
    12181 aaggaacctg gccgtcaaat tccatcgaaa atacagttgt tgtatttgga aggataggag
    12241 tgtgaagtgc cacttccaat attgtttcat cagatttctc cgtcttatgg tccaggtttt
    12301 tacccgtttc atcttttagg gatcttactt tcaaaaaccc aatctcattt gttttaagat
    12361 taaaaattct ttcccgtaca cggttatccg gatctttaat cgtgcgggat cttatatcca
    12421 tcatgcttcc aggtttgaat gcattgaagt acaagtggta atagatgtga gttagagtgt
    12481 caggagaatt attcgtataa attatttttt gtataccttt aaattgatga gtgatatcat
    12541 caaggtcgat ttccatggta taatcaactt gctgctgcca acgatcaggt tgagagtttg
    12601 ccaatgaagg gatcaaaata agagtgacta tcaataattt tttcatcttg ccgattttct
    12661 ttagcataaa ggtacacaga gaatcagtca ttgaaaataa ttatatctgg gtggataatt
    12721 gttcatacca atggttaatc aaactcctcc tcattaaggt tttcttccag gtcttcctcg
    12781 gtttttctta gcttttcacg gcatagtttg ataagttcac cggctctcct tactctttca
    12841 gccagaatat ccacttcaag ttcgccggct tctatttcat taagaatttc ttcaatctcc
    12901 cgaactgctt ctgtatattt aaatttgttg cttttcgcca tcctgataaa tattttttat
    12961 gtcactttca ataaaagaat cgtgtgtttg ggtaaccaac aaatcaccca ttgaaattcc
    13021 ttttacattt ttcagaagaa cattatttat tgttgaaatt gtgaatcccc ttttcaatat
    13081 tgatttggga tcgagcaatc gaagtctttc atcataattt tttaaagctg ttcggtttat
    13141 tgataaaaag gaaaccggaa gtttaatcat ttgagtttca aaaaaattaa gcttagtcac
    13201 atttgactgg atcaaacctc tgcttaataa cctgattgaa ttagcgaatg catccaaatc
    13261 taatcgttca ttctcaattc tggtctctgc tacccggtgg attctttgcc taagactcag
    13321 caattcattt tcaaattccc ggaatccgat taatagaaac tcggcagtcg ccgtgggtgt
    13381 tttcaggcgc gtatgagcaa cccggtctgc aatggattca tcccgttcat gtccgatccc
    13441 cgtaataacg ggaattggga actgagcaat atgggaacaa atatcatagg aatcataaca
    13501 gtccagatcg acctgcgatc cgccaccccg aattattact actaaatcaa aatcgttcat
    13561 tctttggtgt atcctgcgta aggcttcagg aattgaaaat agcgcttcct ggccttgcat
    13621 aattgccttg aacaaatgtg tttgaatgaa aaagttgtca gggttttttt ccagttgatc
    13681 gataaagtct tcatagccgg ccgcggtggg agaacttata atggcaattc tttgtggcac
    13741 aaccggaagc atcagttctc ggttcatatc tatgatcccc tctttggtta gtttttccag
    13801 caccgcggtt ttcttctttt cactttcccc aagtgtgtag gtgggatcaa tatcgcggat
    13861 gttcaggcta aagccgaaca cttcatgaaa ctgaacctgt acattcatca gaagtttaag
    13921 gccggtttga attgactgtt gagttatact ttcaaaccag gcgctgagat ttctatatgt
    13981 ataggcccaa atagtagctt tggcttttgc aattacctca tcattttcct gctcaatcag
    14041 ttcaaggtag caatgacctt tctgattcag gtgcatttcc ccgatttctg ctaccaccca
    14101 gtaggagggc tggaggtgct gcgaaagcac attttttacc agcgaattga gttcaaataa
    14161 ggagtgatgg ttcatcactc caaaataagc aaaccggacg agttgagaaa attagttacc
    14221 caattgatga ctagtttcca ggccttccct catgatttgg ttgattacgc ctttgttgaa
    14281 gattccgtag cacttccata ttaaaatcac gctcagctct aagtaactta atcgcctgaa
    14341 ccggcgttaa tactgtctga atatcagcga taaactcctt ctcaagattc agttcatcct
    14401 gccttgtttt tagctctttc tcaatcattt tttttgcttc atcatccgtt aatccattct
    14461 tattcatttc catgtgcatc atcctttttt caatctgcac attttccctc ttatcattaa
    14521 attgattgta taatggccaa aacttttctg cctgttgggg agttaactca acccggttag
    14581 ttagaaatcc gattctggca gccctcatct tagcttccgg ctcctgcgcg aaggcaggct
    14641 gcattccaaa cagaactgcg actataattg aaatcttttt cattgtaatt ttattaaagt
    14701 aattcatcag aatattttag aagatttaat aatatttcct cctcgatttt atcgctgtca
    14761 aatgtgaaat tttctttcat tatttcaaga taggtatcct catcgatgct aatttcttca
    14821 atgatatcaa cgagggaaat atcagccgat tcaagataat ttatcagtcg ttcatcgctt
    14881 atttcgctta attcttcctg gattaacaat tcggttgcag aaaagtgtac cactgaaaaa
    14941 ataattgcca aaatgatcgt aatagaagcc agagcaattc tgaattgagg tctcccatac
    15001 caaatagggg atttgtccgc gtcaattttc tttcggattt gagccgggag ttcttcaaaa
    15061 tacccttcag gaacctggaa aacttccttt tttggaattt gctctaattt cttcatgtag
    15121 tagttagatg atattatcaa taaaaggttt aatgatcatt taaaaattgt tcaatttttt
    15181 taactgccag atgataactt gctttaaggc ctccaactga agttccggta atttccgcaa
    15241 tttcttcata cttcagttca tcaaagtatt ttaggttgaa tactaatcgt tgcttttcag
    15301 gaagcgtaag aattgctttt tgcaatttct tctgaatttc gtctccggaa atcaacggag
    15361 aattatcaat ctgtgatttc cagttatctg atgactgaga ccatggcagg aaattcctga
    15421 tttttttctt tctgaggaag gatatacatt cattacttgt gattctgtaa atccaggtaa
    15481 acaagctgga atcgcctctg aatttttcca ggttttgata tacttttatg aaaacgtcct
    15541 gggtaatatc gtttgcatca tcatggtcga taaccatttt tctcgccaga agatagactc
    15601 tttcctgaaa atgcgtaaca agaagattga atccatagtc cctggtttca ggattcagta
    15661 gtttatccag aatatctttt tcttccaaat ggatggatcg ttataacaga ctattggaca
    15721 ctagaaatta tttatgatgt gagtgatggc tttgatcaga attattaatg aaaaagcaaa
    15781 tgcgattgca atcacccaga ctttttgaaa gtaggtcaaa tcattgtttg ttccgggaag
    15841 tttatcaaaa atacgttcta ttaatttttt cattaaaatt ttctgatata ctcaagaaat
    15901 cctacattat ctcctttggt ccggacaacc aaaagtggaa tagttgaatc tatctttagc
    15961 attttctcag caaagcttcc tatgaaaatt gcagttgttg atgaaacccc ccttgcacca
    16021 aatattatta catctgcgtt tatttccaca gccatccggt agatagatga cacaaggtgc
    16081 ccgtgagttt ccagggaata aacaggaatg attttcgaac ccttaatatc tatcttctta
    16141 ataaattttt tgtaggattt ctccgcatta ttccgcatta tttctgcaaa ctcttcataa
    16201 gttttccccg tataatgata accggtaggc accgtgtaaa cgtgctggca ataaatttct
    16261 attgtcttct tagtatctga attaagtgcc atattaacag catgttccat tgcaagctcc
    16321 gaatggtttg aataatcatt tgcaaccagt agtttcctgg aagtccgcag gtcatttagg
    16381 acagcatctc ctcctttggg tacaataagt aaattacaat ccgctcttcg tgccacttga
    16441 tgcaatacca caccggaact ttctgccgaa ggctttttgc ccatcactat caggtcaata
    16501 tccagctgct caataagttt aagaatctcc tttgcaggat cgccattctt gacaataact
    16561 ttggaggtgg aatcggggtg catcaccaaa ttttctctta ttgaatcatt aatgtgagat
    16621 ttccgatctt taagagcagc ttccactatt tcaggaaact ctttacgaac ttctttaggc
    16681 actttcatgt gcttaattat attcacgaaa tagaatgctc gcgtagggtt ggaacgtgac
    16741 ataaacgaag caaacttaat tagcagcgga tcaaggtctg aggcgtccag accaagaaga
    16801 atatttcgga atggcttcat gaaagaaata aattaggtgt aaaattaaca aattaatctg
    16861 ctttggaaca ccttaaatta atttgctttc gatacccaat tctccatttt ttcatcttaa
    16921 cttagagtat tacccactgg tagtattttc gttttttaaa cctaacaaaa aacaaatacc
    16981 gggtgagttt acttttgaaa ccgggcctca tcccgattcg tcgggccccc gcaagccggg
    17041 gttaacagtg gagggttgag aaagcggagc agcccaaatc ctgtctatta tcaggtttaa
    17101 taaaccatag acaaccagtg ggttttttta atacagtact ctgaacttta ttgtaccgtc
    17161 tattttcttc aattcctgaa ctacttccct gttgtattct ttatcaatat cggtaattac
    17221 ataaccgatc aattcgttgg tctttagata ttgacccagg atattgatgt tatgagcagc
    17281 gagtacctgg ttgatgcttg ccagaactcc tgaagtattt tgatgaatgt gaattaaccg
    17341 gtgtgcattc tcgagtggcg gtagattcag attcggaaaa tttacactgt ttgtggtctt
    17401 gccggtattt acgtaatcca tgatcttgtt aggaacaaaa ttggcaatgt tctcctgggc
    17461 ttcttcagta cttcctccaa tatggggagt aagaataaca tttggaagtt ccaggagtgg
    17521 cgatttgaat tttttattcc ttcccagagg ttcttcagga aaaacatcaa tggccgcgcc
    17581 acccagttta ttactttgga gtaccttttt aagatcatct aaatcaatta catgacctct
    17641 gctaagattt ataaggatgg catttttttt catccacttc aactgggttg atgaaatgag
    17701 attcttgttt tctttcctgc catccacatg gagagaaacg atatcggcaa gattcagtaa
    17761 ttctttgagg gaattgcact tagtagcatt acccagcgca agtttttcta ctatatcgta
    17821 gtaatacaca tcaagcccaa gagactcggc gatcaccgaa agttgagtgc caatatttcc
    17881 ataaccgatg atgcccaact tcttaccccg gatttcgtgg ctgccaacag cggatttgtt
    17941 ccagttacct ttgtgcatat cagcattgac agcagtaatt ctacgcatca ggtaaatgat
    18001 atgcgcaatg gccagttcta ccacagatcg ggtattggaa aaaggggcgt tgaaaactac
    18061 aattcctttc ttagtacatt catctaaatc aatttgattt gttccgatgc agtacgcacc
    18121 gatagatatc agacgacgag cttgttcaat tatccttttt gtaacatgag tctggctcct
    18181 gataccaaga atgctgatat caggaatttt cttcagcaat tcctcttcgg ataaactttc
    18241 attaatttcc tcaacctgat agccttcctt tttgagcaga tcgaatgctt tggaatgcac
    18301 attttcaaga agcagaactt ttattcgatt cttagggtag gagagtgaac cattcatttt
    18361 atttacaaaa agaaattcat ccaaacttgg tgctacgtga tcggcttgtt ccagaatatg
    18421 tttgcgctcc acattttcgg tgaaagcgaa gaatttattg gaaagaccgg cagctttaat
    18481 ttcataatca gtatacccgt ctccaattac gtaaacttct ccagataatt ttaagctcgc
    18541 caattgctta acctttccgt tgttattcga aagcacattt ttcttgtcaa acccaacgat
    18601 accgccgtcc ttgtcgaact gaaatgagtt ggcaaaaacg tggtcatctc ttattccata
    18661 ctcagaaact attggcacaa tgaactccct gaacccattg gaaataataa aaatatgatt
    18721 gcggtatttg gtgagaaatt ctctgtttct tttgaaggag gggcttactt tttcttttaa
    18781 ttttttgatt aaaggaggta agtgtttctt attcgcttcc aataacctga tgcgctcagt
    18841 caatgatttt ttgaagttaa tttcaccgtg cattcccttt tcagttattg atttgatttc
    18901 tttaagcgct gcttttctct tgttgccttt gagactgatt cctgccagta tatcaagggc
    18961 ttcaacctgg gtgaaagtgg aatcaaaatc aattatgaaa tatttctcgc tcaagagatt
    19021 ctctttagtg gcaaaggtaa taaaaataac ttagtcgcgg tttggtgatt tatttcatcg
    19081 ctttgaaaag gttactccag attggtcaaa aaaaaacaac ctgatgcaaa tgcacaaggc
    19141 tgtcttcttc agataaatct atctctagtg aaccacgacg atcttttggc gcgttatcac
    19201 taaattctca tggtcgattt gaactacgta cattccgttc ttcaatctct tagcatctac
    19261 atttatactt gtggatccag caggtacaat tgaagtcatc attacctttc caaacgtatc
    19321 gaataccctg atggtgagtt cctcagtcac cggagatttg aatcctatgg taaaggacct
    19381 gtctgccggg tttggataaa cggatattcc ttccctggag agcttatctt caatggaggt
    19441 taccaggtta cc
//
LOCUS       DASWHX010000211         3386 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_1336442, whole genome shotgun sequence.
ACCESSION   DASWHX010000211 DASWHX010000000
VERSION     DASWHX010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 3386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3386
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1336442"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            321..2453
                     /locus_tag="VGA21_13905"
     CDS             321..2453
                     /locus_tag="VGA21_13905"
                     /inference="COORDINATES: protein motif:HMM:NF018190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HEX9651652.1"
                     /translation="MATGKVVPTTPANRIEVDPIIDGNVLSDEIWQQIQPVTNLIQNQ
                     PNRGQPSSEKTEIRISYTDKMFYLSVICYDSQPEKLVVSDARRDASLENTDAFLFILD
                     TYNDGQNGFVFGTNSLGVQYDAQVDNEGQGNFNQNRQAGGTIGGFNINWDASWDVKAE
                     TGSYGWSAEFAIPLRTLRFAPGDGKTWGLNFQRTIRKTNEIDYWVEMPIEFDIKRLSL
                     AGKLTGLNLKSPGNLKLIPYGLMRVYRDVNDEPIDTDMDYEFGGDIKYSITPSLTLDL
                     TYNTDFAQVEVDDQQVNLDRFNLFFPEKRPFFLENAGIFSVGSPGEVDLFFSRRIGIS
                     DGLPVPIIGGARLSGKIKNTNLAMLTMFTDDVDSAGIEQNNFSIVRVNQQLSQRSSIG
                     AAFLNREGLGDSNRYNRTYAMDGKLGIGKKATVSGFYSQTKTPDLTGREHAYKLQAQY
                     EWNGLNVNLAYTEVGESFNPEMGFLFRNAFRKPEFLVLKKVRMNGKLGLLEIRPHISY
                     RGYWNFDNFQETGFLHIDNHWDFLQGLEIHTGVNITTEGVVDDFAINPGDTVRAGTYN
                     HVEGQVLIITNASKPLYLNTRSVIGGAFGGIKYQNSGTLGIRIGDNFNSELTYSNNDF
                     NLDESHFTTHVFAGRVSYSFTPSIYLQSLVQYNSVADLWSVNARFGWLQQANAGLFIV
                     YNQVNGGNNPVNRNFIIKYSRVIDLVRR"
     gene            2508..2702
                     /locus_tag="VGA21_13910"
     CDS             2508..2702
                     /locus_tag="VGA21_13910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651653.1"
                     /translation="MKRFKFYDLFHELPNGSLTPKREIIINGISFGPGVLFTRGVSFG
                     EVSINIRVLTSQVRKIMGQL"
     gene            3202..>3386
                     /locus_tag="VGA21_13915"
     CDS             3202..>3386
                     /locus_tag="VGA21_13915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651654.1"
                     /translation="MKLKQFSLPEGHLLSVVILFIFLYQPGLLRAEIKNGYEIELEET
                     RQSLENINTMIKMEGRN"
BASE COUNT         1031 a          622 c          738 g          995 t
ORIGIN      
        1 aattggttta cctggcaaca gtgaactttg agaaacgatg gaagaataca gtgcataact
       61 ggccttccat tttcaattac ttatccttat attttgaaga tcgaattaat ttgaatgaca
      121 cagtttaatg aacattcccc ccggttcggc ctgtcctccg gatgtcacac aaagattgct
      181 ctcaatattt cccaaatttc ccgcattatt aaaagaaaat aataaattcg tagcgctaaa
      241 ctattcccgc tgtattgaaa atgagattat ctctcaccgt tttttgtatt ttctgcgtca
      301 ctccattcac ttacagccaa atggctaccg gcaaagtcgt tccaactaca ccggcaaacc
      361 gaatcgaagt tgatcccata attgatggta atgtattgtc tgatgaaata tggcagcaaa
      421 ttcaacccgt taccaacctg attcagaacc agccaaatcg cgggcaacct tcatcagaaa
      481 aaacggaaat acgaatttcc tatactgata agatgtttta tctgtcggtt atttgctacg
      541 attcccagcc tgaaaaattg gtggtgtcag atgcccgtcg tgacgccagt ctggaaaata
      601 ccgatgcttt tcttttcatt cttgacacat acaacgacgg gcaaaatggg tttgtttttg
      661 gtacaaattc cctgggagtt cagtatgatg cccaggtgga taatgaaggg cagggaaatt
      721 ttaatcagaa ccggcaggct ggcggtacca tcggaggttt taatattaat tgggacgctt
      781 cctgggatgt gaaagcggaa acgggaagtt atggctggag tgctgagttc gccattcctt
      841 tgagaacttt acgatttgca ccgggtgacg gtaaaacctg gggtttgaac ttccagcgca
      901 ctattagaaa aaccaatgaa attgactact gggtagaaat gccaatcgag ttcgacataa
      961 aacgactgag ccttgccggg aaacttaccg gccttaattt gaaaagccct ggaaatttga
     1021 aattaattcc ctacggttta atgagagtat accgggatgt taatgatgaa cccatcgaca
     1081 ccgacatgga ttatgaattt ggcggcgaca tcaaatacag tatcacgcca agtcttactc
     1141 tggacttaac ctataataca gactttgccc aggttgaagt tgatgatcag caagtcaatc
     1201 tggatcggtt caatctcttc ttcccggaaa agagaccatt cttcctggaa aatgccggaa
     1261 tctttagtgt tggaagtccc ggagaagttg atttgttttt tagccgccgc attggaatca
     1321 gcgacgggct tccggttccg atcattggag gggctagact gtcagggaaa attaagaaca
     1381 ctaacctcgc catgcttacc atgttcactg atgatgtaga ctccgcaggc attgaacaaa
     1441 ataatttcag tatagtcaga gtaaatcagc agctttcaca acgatccagt attggcgccg
     1501 cgtttctaaa ccgggagggt ttaggcgata gtaatcgtta taacagaacc tatgcaatgg
     1561 atggtaagtt gggtattggc aagaaagcca ccgtctccgg attttattct caaacaaaaa
     1621 ctcctgactt aacgggtcgt gagcatgcgt ataaacttca ggcacagtac gagtggaatg
     1681 gattgaatgt gaatcttgct tatacggagg tgggggagtc attcaatcct gaaatgggtt
     1741 tcttatttcg aaatgctttc cgtaagccgg aatttttagt gttaaagaag gtaaggatga
     1801 atggcaagct gggtttgctg gaaatcaggc cacacatttc ttaccgtgga tattggaact
     1861 ttgataactt tcaggaaaca ggctttctcc atatagacaa ccattgggat tttttgcagg
     1921 gattggaaat tcacacaggt gttaatatta ctactgaggg tgtggttgat gattttgcaa
     1981 ttaatccggg cgatacggtc agggcaggca cttacaatca tgttgagggt caggtactga
     2041 ttattaccaa tgccagcaag cccttgtatt taaataccag gtccgtgatc ggtggagctt
     2101 tcggcggaat taaatatcag aacagcggaa cacttggaat tcgaattgga gacaacttca
     2161 attcagaact tacttattcc aataatgatt ttaacctgga tgaaagccat tttactaccc
     2221 atgtatttgc aggtagagtt tcctactcct ttactccttc tatctatctt cagtcactgg
     2281 tacaatataa cagtgtcgcc gatttatggt ctgtgaatgc caggtttggc tggctacaac
     2341 aagcaaatgc gggattgttc attgtctata atcaggtgaa cggcggaaac aatccggtta
     2401 atcgaaattt cattatcaaa tacagccggg tgattgattt ggtgaggagg tagtgtatct
     2461 acctaaaagg attattgatt tttaaactct tcgtttatat tagcgatatg aaacggttta
     2521 agttttatga tctttttcat gaacttccga atggttctct aaccccaaaa agggaaataa
     2581 ttattaatgg aatttctttt gggccgggtg tcttattcac acgaggcgtt tcttttggtg
     2641 aagtatccat caatataaga gttttgacat cgcaggtgag gaaaataatg gggcaattat
     2701 aattaaaagg gttttacaaa tagcttgatg gaaggaaagg aagaaaatat tagcaaaaat
     2761 aatgagccgg actggaagct ggaattaaag gtttatccga agaaaattta tccttagaag
     2821 ccaaattaat gcaattagaa atgagagccg gaatattatt atcggaattc cggttgcagg
     2881 attattaatt acggttaccg ttgctattga ggttatctta ttccatacgg gaaacaataa
     2941 aagccagaat gaactttacg aagagattaa taaaatcaaa gaatcaaatt tggaaaaatt
     3001 cggaaaactc caagatgaac ttcgcagttt aaacctgcag aatgaatcac tgaaaaatga
     3061 gataaatcag ctccttaaat aaattattga gtgcttggtt catctaatct tattccttga
     3121 atgctcatcc ctattttcga gtgattttta aaacagtttc cattcatact ttagcggtcg
     3181 taaaaattcg atccgtaaag aatgaagttg aaacagtttt cgttgccaga agggcattta
     3241 ttatcagttg ttattttatt catttttctt tatcagcccg ggttgcttag ggcggaaatc
     3301 aaaaatggct atgaaattga attggaggaa acacgccaga gtctggaaaa tattaacact
     3361 atgattaaaa tggaaggcag gaataa
//
LOCUS       DASWHX010000212         4245 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_3546777, whole genome shotgun sequence.
ACCESSION   DASWHX010000212 DASWHX010000000
VERSION     DASWHX010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 4245)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4245)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4245
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3546777"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(163..630)
                     /locus_tag="VGA21_13920"
     CDS             complement(163..630)
                     /locus_tag="VGA21_13920"
                     /inference="COORDINATES: protein motif:HMM:NF020387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HEX9651655.1"
                     /translation="MEVFRLSRSKYANALTASGAAGRWNFDDEYVLYTGASRSLATLE
                     RLAHANTSKLAGDYEMMIISIPNGRGFYHEIRIEDLPGEWRFNSVYSELQKIGSDWYT
                     KLLSPVLKVPSAVIPREYNYILNSTHPDFSKISLNPNEEYFWDARLIEMFLKV"
     gene            complement(639..1145)
                     /locus_tag="VGA21_13925"
     CDS             complement(639..1145)
                     /locus_tag="VGA21_13925"
                     /inference="COORDINATES: protein motif:HMM:NF021255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin Xre/MbcA/ParS toxin-binding
                     domain-containing protein"
                     /protein_id="HEX9651656.1"
                     /translation="MSRKSSFKDEETIGMRKKTKLKRRLRSKGLIPVSHMTLPVSVGS
                     FEIIGALQNEGLSAKYYRQLKTLSGLNDEELSDLLNINVKTFRKYRDTKGEVDVKPPQ
                     KEQVVNLLGLYRHGIDVFGGIKEFAEWLDKENFFFDGSKPLTYLKTLTGIKFVDRRLT
                     GLEYGDNA"
     gene            complement(1267..2364)
                     /locus_tag="VGA21_13930"
     CDS             complement(1267..2364)
                     /locus_tag="VGA21_13930"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013452874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEX9651657.1"
                     /translation="MITRPTLLLDKQKCLANISTMVNKAKKAGVVLRPHFKTHQSHEI
                     GRWFREIGIDRITCSSLSMANYFAEDGWSDITIAFPVNVLEIETINKLAAKVKLNLLV
                     ETVEAVELISGKLTSKINLFIKIDAGYGRTGIDYMNLKKIDNVIEAISRHDYLNFSGF
                     LEHAGHSYNARGQEEIMKVHFETLENMATLKARYMQAFPEPVISVGDTPTCSVAEDFG
                     AADEIRPGNFVFYDLMQEQIGACDYEQIAVIMACPVVALHPERNEIVVYGGGVHFSKD
                     RIVNNGKTVFGKVVENNGAGWGDVIEDTYVKSLSQEHGIIKATDSFLKRKKIGDIVKI
                     LPVHSCMAADLMDGYLTTDGEWLEKRVNSLS"
     gene            complement(2443..>4245)
                     /locus_tag="VGA21_13935"
     CDS             complement(2443..>4245)
                     /locus_tag="VGA21_13935"
                     /inference="COORDINATES: protein motif:HMM:NF026253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HEX9651658.1"
                     /translation="GIYRDGANYYYGVLGVLNNMSNVVIPFSYKKIEKISESLFRVKN
                     EANRYAIFSADGNNLTGFTIDRTENKDGYLYITSLGKSGLMTLKGEIIFAPEYAEIFI
                     DKNQPKALAISSWSILDKNGKIQRNFQVENMSPYSDAYWKISGNGYEGIMDLNGKMLT
                     NHEINRIGKLQDDVSIIECSGKFGLIRSNGKIIIPARFDSLIYADGFIYTRFKENQEM
                     GWAVMDTFAVTKSKILYDDIRLYQEKLFAVKKKGKWGFIDRTGEEIVACVYDEVGIPK
                     NNLVKIKFHGEYGIITLDNEWVVLPKKYKVELVGDSTYILKNPYQNSLHNFRGEMIYF
                     TNYILKNESGYLSENIGDSTIWFIDLGGRMVDQTSPMTTVSYDDGNITIVRRNSKLGA
                     MDHNGKIIIDFNNDFEDLHSPSEGYFGIKKNGNYGFIDFDNRLRIANRYENVKPFCNN
                     LAAVKIRNKWGFIDKKEQLVIQPFYDEVESFNQVVSIVKKDGKSGILKIDGELEQRLE
                     FDEIKRLPNGKYLIIKDEKFGMLNENGRQIITPKFDEIHDTDTDYLIVRNGGKYGIIA
                     LNGVPLIPPTYDELIYDKAKGVYLAKIRGQWAMK"
BASE COUNT         1233 a          940 c          661 g         1411 t
ORIGIN      
        1 gtcttgataa aaaaacaaat attgaaacta agtggagttc ttgcaggtta gagcgataaa
       61 ttcaggccag atattcacca aaagaccagt acatttttcc ctctcattgt cttcaatttc
      121 caaggaccct tagtataaat accatattat ttccattccg ttctaaactt tcaagaacat
      181 ttcaataagt cttgcatccc aaaaatactc ttcatttggg tttaaactta ttttgctgaa
      241 atcaggatgt gtactattta aaatataatt atactctctc ggtattacag cagatggcac
      301 ttttaaaacc ggtgagagta gctttgtgta ccaatccgaa cctattttct ggagttcaga
      361 ataaacagaa ttaaacctcc attcaccagg aagatcctca attctaattt catgataaaa
      421 acccctgcca ttaggaatag atattatcat catttcataa tctcctgcga gcttggaagt
      481 attcgcatgt gctaaccgct ctagtgttgc cagggaacgt gacgctccag tataaagaac
      541 atattcatcg tcaaaattcc acctgccagc tgctccggat gcagtaagag catttgcata
      601 tttactcctg gataatctga atacttccat tggccggact aggcattatc accatattcc
      661 aagccggtca atctcctatc tacaaattta atccccgtaa gtgtctttaa atatgtaagt
      721 ggtttgctgc cgtcaaagaa gaagttctcc ttatccaacc attccgcaaa ctcttttatt
      781 ccgccaaaca cgtcgatgcc atgccgataa agcccaagca aattaacaac ttgctccttt
      841 tgaggtggct taacgtccac ttcacctttt gtatccctat atttccgaaa ggtttttaca
      901 tttatgttca acaaatcaga aagctcttca tcattcaatc cagacaaggt ttttagctgt
      961 ctataatatt ttgcacttaa accctcattt tgcaaagccc ctattatctc gaatgaacca
     1021 actgacaccg gaagtgtcat atggcttact ggtattaatc ccttagacct caacctgcgc
     1081 ttcaacttcg ttttcttacg cattccaatt gtttcttcat ctttaaaaga gctttttctt
     1141 gacatattca aattttaatt tcaaaaaaca acttagtcca atattaccga taaaaaaagg
     1201 caatcttgcc tttttttgaa tattattaat tatcatttaa agataaaagt catttaggaa
     1261 aacctgtcaa ctgagcgaat tcaccctctt ctccagccac tccccgtccg ttgtcagata
     1321 tccgtccatt aaatccgcgg ccatacagga atgtacaggc agaattttca caatatcgcc
     1381 gattttcttt ctcttcaaaa atgaatccgt ggctttaata ataccatgtt cctgcgacaa
     1441 acttttcaca taagtgtcct caatgacatc gccccagccg gcaccgttat tttcaaccac
     1501 tttaccaaaa accgtttttc cgttattcac gattctgtct ttcgagaaat gaactccgcc
     1561 tccataaaca acaatttcat ttctttccgg gtgaagggca acgacagggc aagccattat
     1621 aactgcaatt tgttcataat cacaagcgcc gatttgttcc tgcattaaat cataaaacac
     1681 aaaattacct ggtctgattt catcagctgc accgaaatct tctgctacac tgcaagtggg
     1741 agtatctcca acggaaatta ccggttccgg aaatgcttgc atatacctgg ctttaagcgt
     1801 ggccatgttt tcaagggttt caaaatgaac tttcataatt tcttcctggc ccctggcatt
     1861 atagctgtgt cccgcgtgct ccaaaaagcc ggaaaagttt aaataatcat gtcggctaat
     1921 ggcttcaata acattgtcta ttttcttaag attcatgtag tcaattccgg ttcttccata
     1981 gcccgcatca atctttataa aaagatttat tttagaagtc aatttgccgg aaatcaattc
     2041 caccgcttca acggtttcca ccagcagatt caatttcact tttgcagcca gtttattaat
     2101 ggtttcaatc tccagtacat taacaggaaa tgcaatcgtt atgtcgctcc atccatcttc
     2161 agcaaaataa tttgccatgc ttagcgacga acaggttatg cggtcaattc ctatttctct
     2221 gaaccatctt ccaatttcat gagattgatg ggttttaaaa tgagggcgta aaactacacc
     2281 ggcttttttt gccttattta ccattgttga aatattagca aggcattttt gtttatcgag
     2341 aaggagcgtg ggtcgggtaa tcatgttttt agtagtatca ggatatgtga atggtattgg
     2401 ttttcttctc tggttccggt taatatcaaa ttaaattgaa ggttatttca ttgcccactg
     2461 tccgcggatt ttggccaggt atactccttt tgctttgtca taaatcaatt catcgtaagt
     2521 aggtgggatt agcggaactc cgttcaatgc aattataccg tatttcccac cattcctgac
     2581 aatcaggtag tccgtgtcgg tatcgtggat ttcatcaaat ttaggagtaa taatctgacg
     2641 accattttca tttagcattc cgaatttttc atcctttata atcaaatatt ttccgtttgg
     2701 caatcttttt atttcatcaa attcaaggcg ttgttccaat tctccatcta ttttcagaat
     2761 gccagattta ccatctttct ttacgatgga aactacttga ttaaaagatt ccacctcatc
     2821 gtaaaagggc tgaattacga gctgttcttt tttatcaatg aagccccact tatttcttat
     2881 cttaactgct gccagattat tgcaaaatgg ttttacattt tcataccggt tggcaatcct
     2941 taaacggtta tcaaaatcaa tgaatccata attgccattt tttttaattc cgaaataacc
     3001 ttcactgggc gaatgcaaat cctcaaaatc attattaaaa tcgataataa ttttgccgtt
     3061 atggtccatt gcccccagct ttgagtttcg tctgacaata gtaatattac catcgtcata
     3121 actgaccgtt gtcataggtg aagtttgatc caccattctt ccacccaaat caataaacca
     3181 aatggtactg tctccgatat tttcacttaa gtaaccggat tcatttttta atatataatt
     3241 ggtgaagtaa atcatttcac ctctaaaatt atgcaaacta ttttgataag gattctttaa
     3301 gatataggtg gagtcgccta ctagttctac tttgtatttt tttggcagaa ctacccattc
     3361 gttgtccagt gtaataatgc catactctcc atgaaacttt attttaacca gattattttt
     3421 aggaattcca acttcatcat aaacacaagc tactatttct tcaccggtac ggtcaataaa
     3481 cccccactta cctttcttct ttacagcaaa taatttttcc tggtaaagtc gaatatcatc
     3541 atatagaatt ttactttttg ttactgcaaa ggtatccatc acagcccagc ccatttcctg
     3601 attttcttta aaccgggtat aaataaatcc atccgcataa atcagggaat caaatcgggc
     3661 gggaataata atctttccat tacttcggat taaaccgaat tttccggagc attcgatgat
     3721 ggaaacatca tcctgaagct taccaattct attgatttca tgatttgtga gcattttccc
     3781 attcaaatcc ataatcccct cgtatccgtt accggagatc ttccagtagg catccgaata
     3841 cgggctcata ttttcaacct ggaaatttct ctggattttg ccattcttat caagaatgct
     3901 ccaactggaa attgcaagcg ccttgggttg atttttgtct ataaatattt ccgcatactc
     3961 aggagcaaag atgatttctc ccttcaaagt cattaaaccg gatttgccaa gagaggttat
     4021 atacaggtag ccatccttat tttcggtccg atcaatggta aaccctgtga ggttatttcc
     4081 atcggctgaa aaaatcgcat accggttagc ctcgttctta acccggaaaa gagattctga
     4141 aattttctca atctttttat agctgaatgg aatgacaacg ttgctcatgt tattcaaaac
     4201 gcctaaaacg ccataataat aattggctcc atctctgtaa attcc
//
LOCUS       DASWHX010000213        29279 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_3548967, whole genome shotgun sequence.
ACCESSION   DASWHX010000213 DASWHX010000000
VERSION     DASWHX010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 29279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29279
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3548967"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..335
                     /locus_tag="VGA21_13940"
     CDS             <1..335
                     /locus_tag="VGA21_13940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010856600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-dependent epimerase"
                     /protein_id="HEX9651659.1"
                     /translation="NTRLPMMYMDDAERATIELMDAPASNIRIRSSYNVSAMSFSPAE
                     IAKSIAHKIPGFRITYSPDFRQKIAESWPASIDDTKAKEDWNWQHEFDLEKMTAQMLA
                     NLEQTINK"
     gene            340..1122
                     /locus_tag="VGA21_13945"
     CDS             340..1122
                     /locus_tag="VGA21_13945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010608108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX9651660.1"
                     /translation="MKNYNNLLLETKEGILTLIISRPSSLNALNLETMKELDEVFDEI
                     LTDEQVQGVILTGAGEKSFVAGADITELANLDAKSAKSFAQNGHRILAKIENCPKPVI
                     AAINGYALGGGCEIAMACHMRTATNTAIFGQPEVNLGVIPGYGGTQRLTRLIGKGKAI
                     ELMTTGDSIKADEALSLGLVNHVFNSQSELIDGTIQLMRKILSKAGFAISMVIDSVNA
                     YDDLRNGYESEAENFARSVDTGDFKEGTRAFLEKRKPQFKGK"
     gene            1135..2631
                     /locus_tag="VGA21_13950"
     CDS             1135..2631
                     /locus_tag="VGA21_13950"
                     /inference="COORDINATES: protein motif:HMM:NF014051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEX9651661.1"
                     /translation="MGALKQLAGETALYGVSSIFGRLLNYFLVPLHTAIFIPSEFGSI
                     TLLYSYVAVFNVLYTYGLETAYFRFITKSRDPKIYDLACTSVLSSSLVFSAILIYFAP
                     DIARLLGEPGKGYIIQWLAVILFVDAFSSIPFAKLRIENRPLKFVIAKLGNILLIVFF
                     NILFLLVFKGIYEERFLVSLKPFIGDIYTPGLGIGYVFLANLLGSAGLLFFLFSEIKK
                     FSFYFNWTTLKPILIYSIPIMITGLSGMLNDNLDKIMIASYLPADFYPQHNPQGALGI
                     YGAVFKLSVFMMLGIQAFRYAGEPFFFSQAERRDSAMLYASVFHYFVVAGSVLMIAVS
                     VNADLIAFVFLRNPEFRLALYLLPYLLLAKLFFGMYINISIWYKLTDKTMYGVYFSVA
                     GTIITLVLNLILLPIIGYLGSAIAMLSCYSSMCVLAYYFGQRIYPVPYKLWPVFAYLA
                     ISTIFSVGSFFVQHTSFAVDSALNIAFTIVFTGIIFLFEKKRWKRNHA"
     gene            2733..4445
                     /locus_tag="VGA21_13955"
     CDS             2733..4445
                     /locus_tag="VGA21_13955"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9651662.1"
                     /translation="MNYCTNKIQNFFIPILVIISLESSLAQRNEPGEDQNKEWRTEYF
                     FTEGEKFYLLEDYAKSLESFQKAYDLSPNNPAVNYKLAQISAKAEDNSKALFYINKSI
                     TGSPENKYYYLLASELHTRNGDFDSASETYKKMIESIPGTDEYLFELAAIYLYDRKLD
                     LALKTYNQIESKLGVSEESSFQKQKIYLDQNKTDLAIAEMEKLIRTFPDEPSYHARLG
                     EMLLSSNKTEEAIALLKTVINDYPHYSRARLMLSDAYEKNGELELSRQNLMIAFEDPA
                     LDSNLKLQILNTYRAGDRGNRDFVIELTETIARAHPENPTIKVVLGDINLEYDNKEVA
                     LSYYLEAVEIDPSVFNVWDNILKLEYGLNRIESVIKHSEEAMELFPNQYSPYFFNGVA
                     NIRSKNFDEGIYAVNETKKRTSDPGIITQCNLLLGDAYFELKEYQKSDQFFETVIASD
                     PDNNSALNNYSYYLSLRKANLNRAKELSSRLIANNPDNPTYLDTHAWVLFQKEEYEEA
                     RKIIEKAIRSPNANAIHFEHYGDILFKLGKEAEAVEQWKQAKKLNSNSELIDKKIADR
                     KLYN"
     gene            4408..5184
                     /locus_tag="VGA21_13960"
     CDS             4408..5184
                     /locus_tag="VGA21_13960"
                     /inference="COORDINATES: protein motif:HMM:NF025490.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4292 domain-containing protein"
                     /protein_id="HEX9651663.1"
                     /translation="MTRKLQTGNYITKLSIILAAVGILFSCSKRTIGISESIDEGETT
                     IHMVDFQYLSIRSKLLLTESETKEAVILTRMKKDSVIWFNVSGSMGIQGLRAILTTDS
                     VKILDRVTKEYSRFSYEELSEKLNFKVDFNLIQSLIVGNVPQGTSTGDRIDPGKDKFT
                     FKITRPGITVTNQIHSMLMKPERILLKEDERLNSLTFDYSVFELTDARPFPMRVIARL
                     IYDLNGLVVEEIFDAQHTKVEFSDKPLKFPFNVPNKYDSR"
     gene            5156..6382
                     /locus_tag="VGA21_13965"
     CDS             5156..6382
                     /locus_tag="VGA21_13965"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEX9651664.1"
                     /translation="MSRINMIRDNKVIFIIGCFLFCCLPFSVAAQKSKTQLEKEKSEN
                     LRKITETQRILQETENRKETSMGQLQAINEQIKIRQDLIKAIEGEITLLNQEIDDTNE
                     IIESLNDDLDNLKEEYASMVYAAYKADQGFEKLIFIFSASSFNQFMMRLKYMEQYGIE
                     RKNQVKEIEFVTESLLDQLDYIRKKREQKNDLLNEQITENQKLTSLNVKQKSAISSLA
                     KKESQLRKELAERKTANERLEKMIATIIAEEIRKSSASANSNKLTLTPEAARLSESFA
                     GNAARLPWPVESGFISRGFGEQPHPILKRVKTNNKGVYIQTKENEQIRAVFDGVVTRV
                     IEVPGMNYAALVRHGEYMTVYANLRAVDVKPGQQINIKDPIGTVFTDAEGISEIYFQV
                     WKNNDILNPQSWLSRK"
     gene            6474..6704
                     /locus_tag="VGA21_13970"
     CDS             6474..6704
                     /locus_tag="VGA21_13970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014798032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEX9651665.1"
                     /translation="MFNVLGFFGGLGGWEILLILMVLLIFFGAKRIPELARGLGKGIR
                     EFKDATKEIKNEIEEGVKEDPKPPKEENKTSS"
     gene            6848..8263
                     /locus_tag="VGA21_13975"
     CDS             6848..8263
                     /locus_tag="VGA21_13975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008202349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEX9651666.1"
                     /translation="MRKIQILALFLVFIFISDAYSQDFPFDSASLDFVWSQPREELDH
                     FPNEINYETIESRIGTIHTEIPINYNDKVYAFIKYFTEKDREYTRMVLKRSTYYFPIF
                     EEVLRKHNLPQEIKYLSIIESGLNPRAISRVGAAGLWQFMPLTGRYYKLHQDWYVDER
                     MDPYKATEAACLYLKQMYDMFGDWELALAAYNTGPGNVRKAMRRSGKKSFWDIYRYLP
                     RETRSYVPQWAAMVYTMNFAEDHNFFVEDEFEYEIDYDTIMVKQFLNIEILANHLDLC
                     LEDLQKLNPSLKRNALPENTNLYPVKIPTDKLNYFIANRVVILDSSSYGKKHFEQLAR
                     NTPGSTYGRDKIVYRVKSGDVLGTIAMRYGIGISDLRRWNNISGNLIRVGQRLDIWML
                     PGAFEKQPNIVNSKPETVTPQILDYAGSKVYLVQSGDTLWDISKKFDGLTIEKIKELN
                     NLTSNKITPGQKLIIQKNSDD"
     gene            8256..9332
                     /locus_tag="VGA21_13980"
     CDS             8256..9332
                     /locus_tag="VGA21_13980"
                     /inference="COORDINATES: protein motif:HMM:NF027451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4837 family protein"
                     /protein_id="HEX9651667.1"
                     /translation="MIKRITLGFITVLFISCGTQRNGGLLAPASGDWGEILLVMDSTK
                     WAGDLGNEIKATFQPNVEGLPRAEPLFTIRYINPLKLNGVLKSSRYMIFVTSFQSQTR
                     QSTQLKNYFTSESIGTIMEDSTRFLHINENEFAKGQKVMYLFGKTDRILIKNIRENRK
                     SLQGFFNNQEQIRMLSSLFAAKEVKGINKHLLDKHQFTLRSPFGYQIADERENFIWLR
                     SFGAEIDKNIFISYKNYSSEDQLKPESLITWRDEIARQYIFEDPKRPQTFVLTEKRIP
                     VVSEITNINKHYSVELKGLWRTNNLSMGGPFISYSLVDEELNRIYYIEGFLYSPGKAQ
                     RDLVRELETILSTFQTESDLRKTS"
     gene            9389..11656
                     /locus_tag="VGA21_13985"
     CDS             9389..11656
                     /locus_tag="VGA21_13985"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009193712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HEX9651668.1"
                     /translation="MSAKIRKQDALNYHESDPPGKIEVNPTKLLSTQRDLALAYSPGV
                     AEPCLEIAKNPQDVYRYTSKGNLVAVITNGTAVLGLGDIGPEASKPVMEGKGVLFKKF
                     AGIDVFDLEIDAKDPDDFIKIVKSLQPTFGGINLEDIKAPESFYIEEVLRREMTIPVM
                     HDDQHGTAIISAAALLNALVVARKKIGNVKVVINGAGAAAIACTKLWISLGVKRSNVV
                     MCDSKGVITKRRNNIDESKRQFATYRHLKTLHDAIKGADVFLGLSKADVLKPKDLKSM
                     AKKPIVFALANPNPEIEYDVAIKTRKDIIIATGRSDHPNQVNNVLGFPYIFRGALDVR
                     ANAINEEMKKAAVIALANLAKEPVPDIVKKAYGDDSLKFGANYLIPKPLDSRLLTTIA
                     PAVAKAAIKSKVAGIKVKDWEEYNIFLQKKIGIDTRLINRIISRAKRDPKRVIFTEAN
                     NYKILKAAQIIKDENIGKPILLGKKKEILNLIKIHDLDLKNVVIIDPYEEDNKREVYG
                     KILYESRKRKGLILSQAVKQMRDRNYFGPMMLHLGEADAIISGLTQDYPKTIRPALQI
                     VGVDENVCKVAGMYIITNKRGTFFLADTTVNINPNAEELVDIIGMTARAVHFFDIEPR
                     IAVLSYSNFGSNKGEIPEKTRLAVQLARKKFPGLLIDGEMQANVAVNTNLQKELYPFS
                     ELIDKGANTLIFPTLMSGNSAYKLLMEIGGAEAIGPVLLGVKKPVHILQLGSSITDIV
                     NMASLAVVDAQSREK"
     gene            11653..12246
                     /gene="ruvA"
                     /locus_tag="VGA21_13990"
     CDS             11653..12246
                     /gene="ruvA"
                     /locus_tag="VGA21_13990"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011583438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEX9651669.1"
                     /translation="MITYVIGKFVEKQPGSLVVESNGIGYKINISLNTFSFIKEKKEG
                     KILTYLHIKEDAHILFGFATESERQIFLNLISISGVGPGTGLMVMSSLSPEEVRRAIV
                     HEDVKTIQSIKGIGGKTAQRIILELKDKMAKTGLTEGMGELPAGINNSLKNEALSALI
                     TLGINKIAAEKSIDFVLKNSGDNITLEELIKRSLKTS"
     gene            12269..19405
                     /gene="sprA"
                     /locus_tag="VGA21_13995"
     CDS             12269..19405
                     /gene="sprA"
                     /locus_tag="VGA21_13995"
                     /inference="COORDINATES: protein motif:HMM:TIGR04189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HEX9651670.1"
                     /translation="MVPQKLRRLLSISVGLFIAINFFLAKVSGSAYGYYYQTQNTKSD
                     SLRIKEDSLNNLQYNPSRLPTFQQRDRFGDPFSNFTSPSPLLLSDPASLKLDLEMDSS
                     NNYTIYEKIGEINYRPTSTITFDEFNRYQTNKLLKEYWKTKSESLDGESAVSGRNLIP
                     PIYVSPVFDRLFGGSYVELEPRGFVTLDFGGKWQRIDNPAIPVRQQRSGGFEFDQQIS
                     MNVVGKVGEKLQVTANYDNNNSFDFENNLKVEYTGYEEDIIKKLEIGNVSLPLNNSLI
                     SGAQNLFGLKTQLQFGKLFVTAVASTQRGKTDALEIESGGVQKRDFELRASDYDENRH
                     FFLSHFFRQNYERWLSQIPQVSSGLNVTRVEVYVLNKNSTTQSLRNIVALMDLGEGFR
                     VRRDAGGLPLPVVEPLGGINSPAANNSNGLFLNITDPQISRSSDLIKETLENQFNLIN
                     STDFEVVNSARLLDPSEYFIHPQLGYISLYRKLQNDEVLAVAYEYTYNGRPFKVGELR
                     EDYTARPENEVAYLKLLRPRKINIRDQFNKIIPTWDLMMKNIYSLNSNQISKEGFQFR
                     IIYRDDRTGIDNPSLHEGIETKDVPLIGLLGLDKLNPSNDPPPDGNFDYVEGLTINPV
                     SGLIIFPVLEPFGEHLRKIFERNNEQDLIQKYAFDTLYATTKADAELQAERNKFFLKG
                     SFQAGSSSEIILPGINIAEGSVRVLSGNTPLQEGIDYTVDYNFGKVTIINEGVVNSGN
                     RLKITYEKADLFNFQSRTLLGTRMDYRVSNDINFGGTILYLNERPLISRISIGDEPTR
                     NMKYGFDVNVRKDSRLITKMVDALPFLQTKELSAVTFSGEFAQIVPGTSNIVDGEGTS
                     YVDDFEGIATPFHLGNTHQAWAIASTPVTPDNKFDPSGNMRNDLSFGYKRAKIAWHVI
                     DNVFYRSGRSLRPDNIGDQELSNHYVRGVRPQEIFPARDNEIITGFEGIFDIAYFPTE
                     RGPYNFNPSFISSDPKSNWGGITRPVTSEVDFDKSNTEYLEFWLLDPFIDGTNGRVLD
                     GSLNTNNTTGGELIFNLGSISEDVLKDGKHAFENGLPADGSLNNVTQNVWGRVTDATY
                     LNDAFDNSAGARVNQDVGLDGLKNADEQALYPDFSALADPAADDFRHYLDPSFDQLDA
                     KILERYKDFNGLEGNTPLSSTFSNSQFPDNEDLDSDNTLNDLEEYYEYSVSLNPGQLT
                     IGSKYIVDAVTSNVDLPNGSRETVTWYLFRIPVRNFDRKIGNIQGFKSIRFVRTYLTG
                     WEQPVVLRMSKFRLIGSQWRRYTRSLEENRFDQLPETDFTDFTVSVVNIEENSQGGGV
                     NSPYTLPPGFRRDRDNTSVIERNLNEQSMRLCVEDLSDKDARAVFKNVDIDLINYGSI
                     KMFIHAESDNAVDDEITGFLRLGTDFTENYYEIEVPLKITPADVSTNDPNIQRLVWPL
                     ENEINIDLDLLYQLKARRNRTRNPDEFGFRYSEPYTDGLRNYNITIVGRPELSTILGL
                     MIGVRNPGSPDRSSKSVCIWANELRVSDFDRKAGWAANANVNVKLADLGTISGSTRYT
                     TPGFGGVQTKISERERKETLEWDIATNLALDKLLPRGTGLKIPMYASYQQSVITPEFD
                     PKDPDIPLEQTLATISNTEEERNYKNIVQDKGTRRSLNFTGIRKTRNNPESRSDFWDI
                     ENFSFTYAYSDFKRSDFNIESYLLKNQRGSIDYNYAYDPLYIEPFKNVKFLNSPHLKL
                     LKDINLNLLPNSFGLRGELDRRFIKTQYRNENLDIGSIEPTYEKSFFFNRNYTLGWNL
                     TKAISLNYTARTNAIIDEPEGDIETQEEKDEIWDNILDLGRTKTFTQNVNASYKLPFD
                     KLPLIDWVSADYQYTAGYIWTAGSVNLRDSLGNIIRNDRTNNVSGKLDFLKLYNKNKF
                     LKDINSPVKRPVDPKADTVQRPNFGALKGVLRLIMSLRSINMTYSLNESTILPGFLPE
                     PSYFGQDDGLSAPGWGFVFGSQDNEIKRRAVENNWLAQGTGLTNPFTQRQTIDIGLRA
                     TLEPFNDFKIQLDAKKTKSSTFQEIFRYDEDSLTFRSLNPTRSGTFSISYISINTAFK
                     KDRSDNSSPLFDDYIAYREVIRQRLNDLNSSGEYNLNSQDVLIPAFIAAYSGKSAEEI
                     KLSPFPGTPLPNWRVDYNGLSKVEALKDIFSSVTLTHAYNSTYSINSFTNSLLYEEGV
                     GLDKDIEDYPLASIVNENGELVPIYIINQVVITERFSPLIGLNVRTRSRLTAKIDYKI
                     ERTMTLNLSNTQITEIKSNDIGFDFGYTTSNFTLPFRVQGRTVALKNDLTFRMNFSVR
                     DTRTHQRRIEEGSTITNGNVNFQLRPNINYVLNQRLSLQVYFERNINEPKVTNSFKRA
                     TTAFGVQIRFSLAQ"
     gene            19457..19837
                     /gene="gcvH"
                     /locus_tag="VGA21_14000"
     CDS             19457..19837
                     /gene="gcvH"
                     /locus_tag="VGA21_14000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010853093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HEX9651671.1"
                     /translation="MNFPEELKYTKDHEWIKIEGDEATVGITDFAQSELGDIVYVEIE
                     TAGKSLSQGDVFGTVEAVKTVSDLFMPVSGEVAQVNADLQNKPEIVNNDPYGKGWMVK
                     LKISNSNETKGLLSAAQYKEVIGQ"
     gene            19841..20212
                     /locus_tag="VGA21_14005"
     CDS             19841..20212
                     /locus_tag="VGA21_14005"
                     /inference="COORDINATES: protein motif:HMM:NF037970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEX9651672.1"
                     /translation="MINKYSIASLVWAIFTGTLTSLPGTLIPRITGTANFILDNVSHI
                     LFFLILGFLVGKSLLFQFPSFGPQKAFFLTFFICIGYGFALELVQIPIPERVFEWSDV
                     FANGFGAIFGASISVLLKKAN"
     gene            20277..20951
                     /locus_tag="VGA21_14010"
     CDS             20277..20951
                     /locus_tag="VGA21_14010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014018538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEX9651673.1"
                     /translation="MEAKKNPKADLTRTSGLFLNVGLVISLLLVISAFEYRFYDDGSL
                     VNLGQVKDEFEDMLEIPPTEQPPPPPPKIQQPEIIEVPDEEEIEEEIEVDLDVEITEE
                     TVIENIIIDEAPDEEVADDIFNIVEDQPDFPGGKSAFYDYVAKNMKYPTTARRMGTEG
                     RVYVQFVVDKSGNITQVEVIRGIGAGCDEEAERVVKASPKWKPGKQRGQPVRVRMVLP
                     ITFKLN"
     gene            21142..21426
                     /locus_tag="VGA21_14015"
     CDS             21142..21426
                     /locus_tag="VGA21_14015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651674.1"
                     /translation="MEARKNPKADLNKTTGLFLNVGLVLSLLMVISAFEWRFYDDGSL
                     VDLGQVKDEFEDMLEIPPTEQPSPPLTFQKFQTFGKFTESTSIQHSQVVP"
     gene            complement(21398..22276)
                     /locus_tag="VGA21_14020"
     CDS             complement(21398..22276)
                     /locus_tag="VGA21_14020"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009579250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HEX9651675.1"
                     /translation="MISSMTGFGTCTKENPNFESTVEIRTLNSRFLDVSIRLPKAING
                     KEMEIRNIITEKLIRGKVTIEVEIRAKNDPITSVAINKELFKQYYEQLRRLADSVIAP
                     HDDLFRIAIQSPDVMMNETKSEQAEQAWQISRDCLMDALKACDEFRLQEGKALESKFK
                     EYVNTINDLLKKIDEYEPGRIKKIKDRLTGNLQELIGNDRADVNRLEQEIIYYIEKID
                     ISEEKVRLGNHLGYFIEVLEKEDNAGKKLGFISQEIGREINTIGSKANDPDIQMLVVG
                     MKDELEKIKEQLANVV"
     gene            complement(22333..25539)
                     /locus_tag="VGA21_14025"
     CDS             complement(22333..25539)
                     /locus_tag="VGA21_14025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="translocation protein TolB"
                     /protein_id="HEX9651676.1"
                     /translation="MREGRVAICVIILCFFTILNPLYAQQAREKFGKNRVQYKVFDWK
                     FYSSDNFDVYFYEGGDVAAKEAINFLEGEFDRITDVIGYSPYTKTKVFLYNSIADLQQ
                     SNVGINDLDYSEGGQTNFIKSYVEIAHPGTVNDFRQELIYNTSRLLINDMMFGGSLSD
                     MFQSAYLLHLPTWFVEGASAYIANGWSLEMDDYIRDFVTAQRRLRLSRLTGEEARLAG
                     QSIWNYIAETYGRSSISNILNLTRIIRNEEKSIANTLGLSFRQFLNDWQDFYIEIEQI
                     VSTDYVDPADALQVLEKNRKGFDYSQVKLSPDATKLAYVRNYNGRYSVWIRDLDTQNE
                     DKVLSGGYKVINQKVDYQSPLLGWADNTTLGVVNYEKARTTLWLYDITTRTRIPTRFE
                     RFEKVHSFNFSGNGRLAIMSADVAGINDLYLVSVTRSRIKRLTNDLYDEINPVFIPNS
                     NIILFSSNRANDTLSVVSESYKDLGDNFNLFLYNLDTTNLTLNRVTNTFSQDVSPLAL
                     NYSDFYYLSDQKGIQNVFNYNLIDSIYSQVSNFSNNIRYYDLNFNLGRAAFVMENENV
                     SNIYIDYNFDRNRSIFTSQTKRQSVKQARFITDRRRQNLVNRSNKVEVDFSKPDSVQQ
                     SKAKEDIIDTDNYVFDKELESKARIDQPESFLSQYRRFQTQKTIFGPLDYETRFSADN
                     LVTSWVIDPMIGFGILIETQMKDILEDHSFTGSVMGTTDFRSGDFVGEYKFLRGMIDY
                     GFRFYRRVMEWPTAEDVPQKYKKNIIELSASYPLSVRSRFTLKPFFGNTKFEDLDFAL
                     LGGQIPPGRAATINRNYVGINSELVYDNSISRGMNIIEGTRVKITFKHWEGITDKDKS
                     FSNLTFDFRHYQKISREFVFATRIFYGKFFGRNPQNYLLGGVDNWLFSKTKNRGDSEN
                     QGSPLYTARNSDNSDLLFLEYVTPLRGFSYNTFYGNDALVLNAELRFPVIKYFYKGPI
                     SSNFFRNLQFIGFYDIGSSWTGPNPFSEKNEVNTLVIDRGIFRSEIKNYRSPWLQSFG
                     AGLRTVILGFFMKFDLAWPVENFERGTPKFQASFGFDF"
     gene            complement(25567..26619)
                     /locus_tag="VGA21_14030"
     CDS             complement(25567..26619)
                     /locus_tag="VGA21_14030"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX9651677.1"
                     /translation="MNLTVYDYIIVGQGLAGTALAVELMRRKKTVLIFNDPDLHSSSM
                     AAAGIFNPITGRRWVTTWKAQEVFACFPKFYKELEKLVNAKFFHEMPVFRFFQNMQEQ
                     NHWLSLPAAEVPAFVQEITRPGNYSEFIKDSYGGILLNNSGYLNVSIFLKALKNYFME
                     LGIYRQEKVTLDNINWQSPFAVNGSTADRLILCLGYEGVRKTPFGDKPFNAVKGELLE
                     VETSIQLPEMIFNGPAFILPQSDGLIKVGATYDFNDETMETTIKAREILLERIHSFFL
                     PEIRIRNQIAGFRPSSVDRRPVAGEHPHNKKLGILNGLGTKGVSLAPYISKLLADKFE
                     NGKELDREIDIKRFFD"
     gene            26671..27321
                     /locus_tag="VGA21_14035"
     CDS             26671..27321
                     /locus_tag="VGA21_14035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013453874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX9651678.1"
                     /translation="MIKIKKFVFNPFSENTYVLFDETREAVIIDPGCSTPGEERELKS
                     YVEKYQLKVIAVLNTHGHIDHVLGNQFVADTWNVKLGMCKRDLPTYKSVISYASNYGF
                     PKYREMEPGFFLDEEDEFEFGVSTLSVIFTPGHSVGHIVFVNQEQQFVIGGDVLFSGS
                     IGRTDLPGGNFDTLIESIQDKLFSLHDSFVVYSGHGPETTIGEEKVSNPFCRIEQE"
     gene            27331..28014
                     /gene="pssA"
                     /locus_tag="VGA21_14040"
     CDS             27331..28014
                     /gene="pssA"
                     /locus_tag="VGA21_14040"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HEX9651679.1"
                     /translation="MKNLPHILTLFNLLSGCLGITFLFQGHLSLGAIMIFAGAIFDFL
                     DGFAARMLNAKSELGKQLDSLADLVTFGVLPALILYTLSANLNDGWISYLPLGVAIAA
                     ALRLAKFNIDDRQTNFFIGLPTPAAALLVASIPFIMDEEHVTGTLNIYSLIGLSVFLS
                     IMMISRVKLMSFKFSHYNWKENEPKYILILLFLVLVFYFSFISIPLIFLLYLIFSVFV
                     KYPETTKKG"
     gene            28101..>29279
                     /locus_tag="VGA21_14045"
     CDS             28101..>29279
                     /locus_tag="VGA21_14045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651680.1"
                     /translation="MFHPQTQKYFTCLLPALLLIFTNTGYSFQPSLSNSILSSGKWIK
                     IEVSERGIYRITKQDLLNSGFSSAGIVPSKIRLFGNGGKVIPQKISDPRPEDLIENAI
                     YITGSNDGTFDTSDVILFFADGPDELRYDQSNKLQYSRNPYSNSSYYFLTISDNDGKR
                     IESRINEGGSFTLIDYYHDLKSHEVDEHNILQALITSYGGSGREWYGERFKENLNQSI
                     SFNIDNLFDAGNSTLITSLLGVSKTSSSFQTKINNILLGDQPITAIPDSRYGVKGQAR
                     VDTFLISNSLLGQIQNAEIQLQYFPGNAIEDAGYLNYLILAAERKLIKGAGSLIFQNL
                     LTNVSGSHTYAIQNLQTPDIIWDITNPKSPVLQEFDFTGDTGMFGITTDSLHRFIAFN
                     S"
BASE COUNT         9083 a         5829 c         5824 g         8543 t
ORIGIN      
        1 aaaatacccg actccccatg atgtacatgg atgatgctga gagagcaact attgaattaa
       61 tggatgcacc cgcaagtaat atcagaattc gttccagcta caatgtttca gcaatgagtt
      121 tctctcctgc ggagattgct aaatccatcg cgcataaaat tccaggcttt cgaattacct
      181 attctcctga cttcagacaa aaaatagcgg aatcgtggcc tgcttctatt gatgatacga
      241 aggcaaagga agactggaac tggcagcacg aatttgacct tgaaaaaatg acagcccaaa
      301 tgttggctaa cctggaacaa acgattaaca aatgaatcaa tgaaaaatta taataatctt
      361 ctactcgaaa caaaagaagg aatccttaca cttattatct ctcgtccttc cagtctcaat
      421 gccctgaatc tggaaaccat gaaagaactg gatgaagttt ttgatgaaat attaacagat
      481 gaacaggtgc aaggagtaat cctaaccggt gctggagaaa aatcgtttgt ggctggagcc
      541 gacataacag aattggcaaa ccttgacgcc aaatctgcaa agtcatttgc tcaaaatggc
      601 catagaattc ttgcaaaaat tgagaattgt cccaaacccg taattgcagc aataaacgga
      661 tatgccttgg gcggtgggtg cgaaattgcc atggcatgcc acatgcgaac ggccacaaat
      721 acggcaatat tcggtcaacc tgaagtgaat ctcggagtga ttccaggtta tggaggtacc
      781 cagcgtttaa cgcgtttgat cggcaaaggc aaagcgatag aacttatgac aaccggtgat
      841 tcaatcaaag ctgatgaagc cttgagtttg gggttagtta atcacgtttt caattctcag
      901 tctgaattaa tcgatggcac aattcaactt atgcggaaaa tactctcaaa agccggtttc
      961 gctatttcta tggtgatcga ttctgtgaat gcatatgatg acctccggaa tggatatgaa
     1021 tcagaagcgg agaattttgc ccgcagtgtt gatacaggtg atttcaaaga aggtaccagg
     1081 gctttccttg aaaaaagaaa acctcaattc aagggtaaat agatcgaatc cgcaatggga
     1141 gctcttaagc aacttgccgg tgaaaccgcg ctctacggcg taagcagcat atttggccgc
     1201 ttgttaaatt acttccttgt acccttacac accgctatct tcattccttc agaatttgga
     1261 tccataacat tactttattc ttatgtagct gtcttcaatg tattatacac ctatggtttg
     1321 gaaaccgctt atttcagatt tattaccaaa tcgagggatc caaaaattta tgatctggct
     1381 tgcacttcag ttctttcatc gagtcttgtc ttctctgcta ttcttattta tttcgcaccc
     1441 gacattgcga gattattggg tgaaccaggc aagggttata ttattcaatg gcttgcggta
     1501 attttatttg tagatgcatt ttcatccatc ccttttgcaa aacttcggat tgaaaacagg
     1561 ccgctaaagt ttgtcattgc aaaacttggc aacatattat taatcgtttt ttttaatatt
     1621 ttattccttt tggttttcaa aggcatttat gaagaaaggt tcctggtttc ccttaagcct
     1681 ttcatcgggg atatttatac cccaggcttg ggcattgggt atgttttcct cgcaaatctt
     1741 ttgggttcag ccggattatt attcttcctc ttttctgaaa ttaagaagtt cagcttttat
     1801 ttcaactgga ccacattaaa gccaatcctt atctattcaa ttcccattat gatcaccggc
     1861 ctttcaggta tgctcaacga caaccttgac aaaataatga ttgcctctta tttgcctgcc
     1921 gatttttatc ctcaacataa tccccaggga gcgttaggaa tttatggagc agtcttcaaa
     1981 ttaagcgtat tcatgatgct tgggatccag gcttttcgat atgccggtga accgtttttc
     2041 ttcagccagg cggagcggag ggactcagca atgctatatg catccgtatt tcattatttc
     2101 gtggttgcag ggtcagttct aatgattgcc gtatccgtta acgcggacct cattgcgttt
     2161 gtatttttac gaaaccccga gttcagactg gccctttact tattgccata tttgttgctg
     2221 gctaagttat tctttggtat gtacattaac atttccattt ggtataagct tacagataaa
     2281 acaatgtacg gagtgtattt ttctgttgcc ggtaccataa ttacccttgt ccttaattta
     2341 attcttctgc caattattgg ttacctgggc agcgcaattg ccatgctgtc ttgttatagc
     2401 tccatgtgcg ttctggctta ttattttggc caacggatat acccagtacc ttacaaactg
     2461 tggcctgtat tcgcatacct ggcgatttca acaatatttt ctgttggctc attttttgtg
     2521 caacatacaa gttttgcggt tgactcagcg ttgaatattg cctttacaat tgtttttacg
     2581 ggtattattt ttctgttcga aaagaaaaga tggaagagaa accatgctta attacataac
     2641 ccacggcaac tcattgttta gcaatttcgt tacctttgaa gtgacttttt ggcgacttcc
     2701 aggcattaac cattgattcg agactttgcc caatgaatta ctgcacaaat aaaattcaaa
     2761 acttttttat cccgattctg gtaataattt ccctggaatc atccctggca caaagaaatg
     2821 agcctggcga ggatcagaat aaggaatgga gaactgagta tttttttaca gaaggcgaaa
     2881 agttttactt gcttgaagat tatgccaaat cactggaatc ttttcagaaa gcctatgatt
     2941 tgagccccaa taacccggca gtaaactata agctggcgca aatctctgca aaggcggaag
     3001 acaacagcaa ggcgctcttt tatattaata aatctataac tggcagtcct gaaaataaat
     3061 actattatct gttagcttct gaattacata ccaggaacgg agatttcgac tccgcatctg
     3121 aaacgtataa gaagatgatc gaatcaatcc cgggaacaga tgaatacttg tttgaattag
     3181 cagccatata tctttacgac agaaaactgg atttggctct taaaacttat aaccagattg
     3241 aaagcaaatt gggtgtctcg gaagagtctt catttcagaa acaaaagatt tacctggatc
     3301 agaataagac agacctcgca attgcagaaa tggagaaatt aataagaacc tttcccgatg
     3361 aaccatcata ccatgcgagg ttgggtgaaa tgttactaag cagtaataaa acagaggaag
     3421 ccatcgcatt attaaaaact gtgattaatg attatccgca ttattctcgc gcaaggttga
     3481 tgctttctga tgcatatgaa aaaaatggcg aactggaact ttcccgtcaa aatcttatga
     3541 tcgcctttga ggatcctgct ttggattcta atttaaaact tcaaattttg aatacgtatc
     3601 gcgcgggcga tcgggggaat agagatttcg ttatcgaact tactgaaact atagcccggg
     3661 cccatcccga aaacccaact attaaagtag ttctagggga cattaatctt gaatacgata
     3721 ataaagaagt cgctctttcc tactacctgg aagcagttga aattgacccc tccgtattca
     3781 atgtctggga taatatattg aaattggagt atggcttaaa cagaatagag agtgttatta
     3841 agcattccga agaagcaatg gaactttttc ccaaccagta ttcaccttac tttttcaatg
     3901 gcgttgctaa tataagaagc aaaaattttg atgaaggtat ttatgctgta aacgaaacaa
     3961 aaaagagaac aagcgatcct ggaattataa cccagtgcaa tttattatta ggagacgcct
     4021 attttgaatt aaaagagtat caaaaatcag atcaattttt tgaaacagtt attgcttctg
     4081 atcctgacaa taattctgca ttaaataatt acagttatta cctttcgctc aggaaggcaa
     4141 atttgaatcg ggcaaaagaa ttatcatcaa ggctaattgc aaataacccc gacaatccca
     4201 cttacctgga tacacatgca tgggtgctgt ttcaaaaaga agaatatgaa gaagccagaa
     4261 aaattataga aaaagctatt cggagtccta atgccaatgc aattcatttt gagcattatg
     4321 gggatatcct ttttaaactt ggaaaagagg cggaagctgt ggagcaatgg aagcaggcaa
     4381 aaaaactcaa ttcaaattca gaattaattg acaagaaaat tgcagacagg aaactatata
     4441 actaaactct ccattatcct tgccgcagtt ggaattttgt tttcctgcag taaaagaaca
     4501 attggtataa gcgaatctat cgatgaagga gagacaacta ttcacatggt tgactttcag
     4561 tacttaagca ttcgctcaaa acttttacta accgaaagcg aaaccaaaga ggccgttatc
     4621 cttacaagaa tgaaaaagga tagcgtaatt tggtttaatg tttctggttc catgggaatt
     4681 cagggtctcc gtgcaatact gacaacagac tcagtcaaaa ttctggacag ggtcacaaaa
     4741 gaatattcca ggtttagtta tgaagaatta agtgaaaaac taaatttcaa agtagatttc
     4801 aatctcattc aaagccttat tgtgggcaat gtgccgcaag gtacttcaac aggtgataga
     4861 attgatcctg ggaaagataa atttactttc aaaattaccc gtccgggaat cacggttaca
     4921 aaccagattc actcgatgct aatgaaaccc gaaagaattc tattaaaaga agatgagcgt
     4981 ctaaattccc tcacctttga ttactccgtt tttgaattaa ctgatgcaag accatttcct
     5041 atgagagtaa ttgccaggtt gatttatgac cttaatggtc tcgtcgttga agaaattttt
     5101 gatgcccagc acaccaaagt agaattttcg gataaacctc ttaaattccc gtttaatgtc
     5161 ccgaataaat atgattcgag ataacaaagt aattttcatc atcggatgct tcctgttttg
     5221 ctgcctgcca ttttcagttg ccgctcagaa atcaaaaacc caacttgaaa aagaaaagtc
     5281 tgagaacctt cggaaaataa cagaaactca gagaattctg caagaaactg aaaacaggaa
     5341 ggagacttca atgggccagc ttcaggcaat taatgaacag ataaaaatca ggcaggattt
     5401 aatcaaagca attgaaggtg aaattacttt acttaaccag gaaattgatg ataccaatga
     5461 aataatagaa tccctgaatg atgacctgga taatcttaaa gaagaatacg catcaatggt
     5521 ttatgccgca tacaaggctg atcagggttt cgaaaaattg attttcatct tttcagcatc
     5581 ttcatttaac cagtttatga tgcgtctgaa atacatggag cagtatggaa ttgaaagaaa
     5641 gaaccaggtt aaagaaattg aatttgtgac tgagtcgctg ctggatcagc ttgattatat
     5701 ccggaaaaaa agggaacaga aaaatgactt gttaaatgaa caaataaccg aaaatcaaaa
     5761 attaacctcg cttaacgtta aacaaaaatc cgcaatctct tctctggcta aaaaagagtc
     5821 acagcttcgc aaagaattag cagaaagaaa aactgcaaat gaaaggctgg aaaaaatgat
     5881 tgccactatt attgctgaag aaattcgaaa aagttcagcc tcagcaaatt caaacaaact
     5941 tactcttact ccggaggcag cccggctaag tgaatctttt gcaggaaacg cggctaggtt
     6001 gccctggccg gtggaatcag gttttatttc caggggattc ggtgaacagc cccaccccat
     6061 attaaaaaga gtgaaaacca ataacaaagg ggtttatatt cagacaaaag aaaatgagca
     6121 aatcagggca gtattcgatg gtgttgtaac ccgggttatt gaagttcccg gaatgaatta
     6181 tgcagccctt gtaagacatg gcgaatacat gacggtttat gcaaatttac gggcagttga
     6241 tgtaaaaccg gggcagcaaa tcaatattaa agacccaata gggaccgtgt tcacagatgc
     6301 tgaaggaatc tctgaaatat actttcaagt gtggaaaaat aacgatattc taaatcctca
     6361 aagctggtta tcacgtaaat agtaacggta agattcgatt taaatcgttc tttctttaat
     6421 caaataatgc atatctttac tatagcgaaa tagaaactta aattaattgt cctatgttta
     6481 atgttttggg gttttttggt ggattaggcg gatgggaaat tctcctaatc ctgatggtgc
     6541 ttttgatatt ttttggagct aaaagaattc ccgaattggc tcgcggactt ggcaaaggaa
     6601 ttcgtgagtt taaagatgct acaaaagaaa taaaaaatga aattgaggaa ggcgttaaag
     6661 aagaccccaa acctcctaag gaagaaaaca aaacttcctc ctgattggct catcaggcga
     6721 aatcccgtat ctcattttat ctttattact ggcaacattc ttgttaaaga aatttagtaa
     6781 ccaataaaaa ctgtctgctt gtagtgatga atgtttccta tatttaacca ttttaatttg
     6841 gacgcaggtg cgtaaaattc aaatacttgc tctgttcctg gtatttattt tcatcagcga
     6901 cgcctattca caagatttcc cttttgatag cgcaagcctg gattttgtat ggtcccaacc
     6961 gcgtgaagag ttggatcatt ttcctaatga aatcaattat gaaaccattg aaagcagaat
     7021 cggtaccatc catactgaga ttcctattaa ctacaatgac aaggtttatg cattcataaa
     7081 atattttacg gaaaaagatc gcgaatacac caggatggta ttgaaaagat ctacttatta
     7141 ctttcccatt tttgaagagg ttcttcggaa acataatctt cctcaggaaa taaaatattt
     7201 atcaataatt gaatcaggcc ttaacccaag ggcaatttca cgcgtaggtg cagcaggact
     7261 ttggcaattc atgcctctca ccggtcgtta ctacaaacta catcaagact ggtatgttga
     7321 tgaacgcatg gatccttata aagcaaccga agcagcctgc ctgtacctga aacagatgta
     7381 tgatatgttt ggtgattggg aactggcatt ggcggcatat aacaccggac ccgggaatgt
     7441 aaggaaggcc atgcgcagat ccggtaaaaa atcgttttgg gacatttaca gatacctccc
     7501 tcgcgaaacc cgatcctatg ttccccagtg ggctgcaatg gtttatacta tgaattttgc
     7561 agaagaccac aacttctttg ttgaagatga atttgaatat gagattgatt acgatacaat
     7621 catggttaag caatttctga acatcgaaat actggcaaac catcttgatt tatgcctgga
     7681 ggatttgcag aaattaaacc cctccctaaa acggaatgct ttgccggaaa atacaaattt
     7741 atatccggtc aaaataccca ccgataagct taattatttc attgccaaca gagttgttat
     7801 acttgactcc agttcctatg gaaaaaaaca ttttgaacaa cttgcaagaa acacaccagg
     7861 cagcacctat ggaagagata aaatagttta tcgtgtcaaa agcggagatg tgctgggaac
     7921 aattgccatg agatatggta ttggaatttc cgatctccgc cggtggaaca atatctcggg
     7981 aaacctgatc cgggtaggac aacgcctcga tatctggatg cttcctggcg cttttgaaaa
     8041 acaaccaaat attgtgaact ctaaacctga aacggtaact cctcagattc tggattatgc
     8101 tggcagcaaa gtgtatctcg ttcagtctgg tgatacctta tgggacatct cgaaaaaatt
     8161 tgacggctta actattgaga aaataaaaga actcaacaat ctaacctcaa acaagattac
     8221 acccggtcaa aaattgatca ttcaaaaaaa tagtgatgat taagagaata acccttggct
     8281 ttattactgt tctctttatt tcttgtggca cacaaagaaa tggaggtctt cttgcacccg
     8341 ccagcggtga ctggggagaa atattgctgg taatggattc tactaaatgg gctggtgatc
     8401 ttgggaatga aattaaagcc acttttcaac ccaatgtgga aggattgccg agagcggagc
     8461 cgctatttac cataagatac attaacccgc taaaacttaa tggcgtactc aaatcttcac
     8521 gatacatgat ttttgtgacg tctttccaaa gccaaacccg gcaatcaact caactcaaaa
     8581 attacttcac ctctgaatcc attggcacca taatggaaga ttctacaaga tttttgcata
     8641 tcaatgaaaa tgagtttgcc aaaggacaga aagtcatgta cctgttcgga aaaacagaca
     8701 gaatactaat aaagaacata agagaaaacc ggaaatctct tcaaggattt ttcaataacc
     8761 aggaacaaat acgaatgtta agctcacttt ttgcggcaaa agaagttaag ggtatcaaca
     8821 agcacctgtt agataaacat caattcactt tgagatctcc atttggatat caaattgccg
     8881 atgaaaggga gaacttcatc tggttaagga gttttggagc ggaaatagat aagaatattt
     8941 tcatctcgta taaaaactac tcatctgaag accagttgaa gccagagtca ttaattacct
     9001 ggcgggatga aattgcccgt cagtatattt ttgaagatcc taaaagacca caaacctttg
     9061 ttctgactga aaaaaggatt ccggtcgttt ctgaaataac aaatattaat aaacactata
     9121 gcgttgaatt gaaaggtctt tggcgtacaa ataacctttc tatggggggc ccctttattt
     9181 cctatagttt ggttgatgaa gaactaaata ggatatatta catagaaggt ttcttgtaca
     9241 gtcccggtaa agcccaaaga gatcttgtcc gggaacttga aaccatttta tcaacctttc
     9301 agaccgaatc cgacttgaga aagaccagct aggtttacgg tttctatccc ggaattttat
     9361 acctttctga ctcaacaagt tattgcaaat gtccgccaaa attcgtaagc aagatgccct
     9421 gaattaccat gaatctgacc caccaggcaa gattgaagtt aatcctacaa aattattaag
     9481 tactcaaaga gatcttgccc tggcatattc tccgggtgta gctgaaccct gtttggaaat
     9541 agccaaaaat ccacaggatg tgtacaggta tacatcaaaa ggcaacctgg tcgctgtcat
     9601 tactaatgga acggcagtgc tgggtttggg tgatattgga cccgaagcat ccaagcctgt
     9661 aatggaaggc aaaggtgttc ttttcaaaaa gtttgcggga atagatgtgt ttgatctgga
     9721 aattgatgca aaagatccgg atgatttcat aaagatcgta aagtcattgc aacccacttt
     9781 tggcgggatt aacctggagg acattaaagc acctgagagt ttttatattg aagaagtttt
     9841 gcggagagaa atgaccattc ctgtcatgca tgatgatcag catggaacgg caataatttc
     9901 tgctgccgcc cttttaaatg cattagtggt agccaggaag aaaatcggga atgtcaaagt
     9961 tgttataaat ggagccggtg cagccgcaat tgcatgcaca aagttatgga tcagcctggg
    10021 agtcaagaga tctaatgtgg tgatgtgcga ttcgaaaggt gtaataacaa agcgcagaaa
    10081 taatattgac gagagtaaaa gacagttcgc cacttaccga cacctgaaga ctcttcatga
    10141 cgccattaaa ggagcggatg tctttctcgg cttgtccaaa gccgatgtac tgaaacccaa
    10201 agatcttaaa agcatggcta agaaacctat tgtctttgct ctggccaatc caaatcctga
    10261 aattgagtat gacgtggcga taaaaacccg gaaggatatc attattgcaa caggaagatc
    10321 agaccatccc aaccaggtaa ataatgtctt gggattccct tatatttttc gtggcgccct
    10381 tgatgtacgt gctaatgcta ttaatgagga gatgaaaaag gctgcggtaa tcgctttagc
    10441 taatcttgca aaagaaccgg ttcccgatat tgttaaaaag gcctatgggg atgactccct
    10501 gaaatttggt gccaattatt taattccgaa accacttgac tcacggcttc ttacaactat
    10561 cgctcctgcg gtggccaagg ccgcaataaa atcaaaagtg gctggtatta aagtaaaaga
    10621 ctgggaggaa tacaatattt ttctgcaaaa gaaaatagga atagacaccc ggctcatcaa
    10681 cagaataatt agccgggcca agagagaccc caaaagagtt atttttactg aagccaataa
    10741 ttataaaatt cttaaagctg ctcaaatcat aaaagatgaa aatattggca agccaattct
    10801 tcttgggaaa aagaaagaga ttttgaacct cattaagatt catgatcttg atttgaaaaa
    10861 tgttgtgatt attgatccct atgaagaaga caataaaagg gaagtttacg gaaaaattct
    10921 ttacgaaagc agaaaaagga aaggactcat tttaagccag gcggttaagc aaatgcggga
    10981 ccggaattac ttcggcccta tgatgctcca ccttggagaa gccgatgcga tcatttcggg
    11041 cctcacccag gattatccga aaacaattcg tccggcattg cagattgtcg gcgttgatga
    11101 aaatgtttgt aaagtagcag gcatgtatat catcacaaat aagcggggaa cttttttcct
    11161 tgccgatact accgtgaata ttaatcctaa cgctgaagaa ctcgtagaca tcattggcat
    11221 gactgccaga gcagttcatt tttttgatat tgaaccaaga attgcagtgt tgtcttattc
    11281 caattttggg tcgaacaaag gtgaaattcc ggaaaaaaca aggcttgcag ttcagcttgc
    11341 gagaaaaaaa tttcccggac tgctgattga tggtgaaatg caggcgaacg ttgccgttaa
    11401 cacaaacctt caaaaggaat tatacccctt tagcgaactt attgataaag gagcaaacac
    11461 attaattttt ccaacattga tgtcgggtaa tagcgcctac aaattactga tggaaattgg
    11521 aggagcagaa gcgattgggc ccgttctgtt aggtgttaaa aagccagtcc atattttaca
    11581 gctgggcagt tccattacag atatagtaaa tatggcgtcc cttgcagttg tcgacgccca
    11641 atcaagagaa aaatgattac gtacgtaata ggaaaattcg tcgaaaagca acctggttcc
    11701 ctggtagtgg aatccaacgg cattggatat aaaataaata tctccttaaa caccttctct
    11761 ttcataaaag aaaaaaagga aggcaaaatt cttacttacc tgcatattaa agaggatgca
    11821 cacatccttt tcggattcgc taccgaatcc gaacggcaga tttttttgaa tttaatatcc
    11881 atttccggag ttggcccggg cacaggtcta atggtgatga gctcgctttc tcctgaggaa
    11941 gtcagaaggg ccatcgttca tgaagatgtt aaaacgatcc aatccatcaa gggaatcggc
    12001 ggtaaaacag cccagcggat tatccttgaa ttaaaggata aaatggcaaa aaccggatta
    12061 accgaaggca tgggagaatt accggcaggt ataaacaata gtttgaaaaa tgaagcgtta
    12121 tctgcattga taacgctggg gatcaataaa attgccgctg aaaaaagtat cgattttgtc
    12181 ctcaaaaact ccggggataa tattaccttg gaagagttaa taaagcgatc gttaaaaact
    12241 tcataaggtg taggattatt agtacgtctt ggtaccgcag aaattacgta ggctattatc
    12301 aatatcagtt ggactcttca ttgctataaa cttttttctt gcaaaggttt ctggtagtgc
    12361 gtatgggtac tattatcaaa cccaaaatac caagtctgat tcattacgga tcaaagaaga
    12421 tagcctgaac aatcttcaat acaacccctc ccggttacca acatttcaac aacgcgaccg
    12481 ctttggagat cccttttcaa atttcacttc cccatcccct ttactcttat ctgatcctgc
    12541 cagtcttaaa ctggatctgg aaatggattc atcaaataat tataccatct atgagaaaat
    12601 tggtgaaatt aattaccggc ctacttccac catcactttt gacgagttca acaggtatca
    12661 gacaaataaa cttcttaaag agtattggaa aacaaaatca gaaagtctcg atggagaaag
    12721 cgctgtaagt ggaagaaatc tcattccccc catttatgta agtccggttt ttgacagatt
    12781 atttggtggc agttatgttg aacttgagcc caggggattc gtgacactgg atttcggtgg
    12841 aaagtggcag cgaattgata atcccgcgat accagtaagg caacagcgaa gcggtggctt
    12901 tgagtttgac caacaaataa gcatgaacgt ggtaggaaaa gtgggggaaa aacttcaggt
    12961 aactgccaac tacgataata ataatagctt cgactttgaa aataacctca aggtagagta
    13021 taccggctat gaagaagata tcataaagaa actggaaata ggaaacgtaa gccttcctct
    13081 caataatagt cttatctcag gggcccaaaa tttatttgga ctgaaaaccc agttgcaatt
    13141 cggtaagttg ttcgtaaccg ctgttgcttc cactcagcgt ggtaagacag atgcacttga
    13201 aatagaaagt ggcggcgtgc aaaaaagaga ttttgaatta cgggcatctg actacgatga
    13261 aaaccggcat ttttttcttt cccacttttt ccgtcagaat tatgagagat ggctttcaca
    13321 aattcctcaa gtaagttccg ggctcaacgt tactcgagta gaagtctatg ttttgaacaa
    13381 aaattcaaca acccagtcgc tgagaaacat tgttgcgctt atggatcttg gggaaggatt
    13441 tcgcgttcga agagatgccg gcggacttcc gcttccggtt gttgaaccct tggggggtat
    13501 taattcgccg gcggcaaata attcaaacgg actatttctc aacatcacag atcctcaaat
    13561 atccagaagc tctgatttaa taaaagaaac tctggaaaat caattcaacc ttattaactc
    13621 aaccgacttc gaagtagtaa atagcgcccg gctccttgat cccagtgaat attttattca
    13681 tccccaactt ggatatatta gtctataccg gaaacttcag aacgatgagg tactggctgt
    13741 agcctatgaa tacacctaca atggccggcc attcaaagtg ggtgagctaa gggaagacta
    13801 caccgcccgg cctgaaaatg aggttgctta cctgaaacta ttgcggccaa gaaaaataaa
    13861 tattcgcgat caattcaata aaatcattcc cacctgggat ttgatgatga aaaatattta
    13921 tagccttaac tcaaatcaaa tcagcaaaga aggttttcaa tttagaatta tctatcggga
    13981 tgaccggaca ggcattgata accccagctt gcatgaaggt atcgagacga aagatgttcc
    14041 cttgattggc ttattgggac ttgataaatt aaatccctcc aatgatccac ccccagatgg
    14101 aaattttgac tacgtggaag gtttaaccat taatcctgtg agtggattga taatcttccc
    14161 tgtactggaa ccatttggtg aacatcttcg aaaaatcttt gaaagaaata atgaacagga
    14221 cctgattcag aaatatgctt ttgacacttt gtacgctaca acaaaagcgg atgctgaatt
    14281 gcaagccgaa cggaataaat tttttctgaa aggaagtttt caagcaggtt catccagtga
    14341 aattattcta cccggaatta atattgcaga aggttctgtg cgggtactgt ctggaaatac
    14401 acctcttcag gagggaattg attatacagt ggattacaat ttcggtaaag tcaccattat
    14461 caatgaaggc gtggtaaact ccggcaaccg actgaaaatc acctatgaaa aggccgactt
    14521 gtttaatttt caatcccgaa ctctgctggg cactcgtatg gattatcgcg tcagcaatga
    14581 cattaatttt ggcggtacca ttctttatct caacgagcgg ccccttatca gcaggataag
    14641 tattggtgat gagcccacca gaaacatgaa atacgggttt gatgtaaatg ttagaaaaga
    14701 ttcaaggtta attactaaaa tggtagacgc gctaccattc ctgcagacca aagaactttc
    14761 cgccgttact tttagcggag aatttgctca aatagttcct ggaacctcaa atattgtaga
    14821 tggagaaggc acctcgtatg ttgatgattt cgaaggaatt gccaccccct ttcaccttgg
    14881 gaacacgcac caggcatggg caattgcctc cacacctgtt actcccgaca ataaatttga
    14941 tccaagcggg aatatgagaa atgacctcag ctttggttac aaaagagcaa agattgcctg
    15001 gcatgtcatc gacaatgttt tttatcgcag tgggcgaagc cttcgtcctg ataatatcgg
    15061 agaccaggag ttatctaacc attatgtacg aggagtaaga ccacaggaaa tttttccggc
    15121 acgcgataat gaaataatta ccggctttga aggaattttt gatattgcct acttcccaac
    15181 agaaagaggc ccttataatt ttaacccttc attcatcagc agcgacccga aatcaaactg
    15241 ggggggcatt acccggccgg ttacttccga agtcgatttt gataagtcaa acacagaata
    15301 cctggagttt tggttacttg atccatttat tgatggtact aatggcagag ttctggatgg
    15361 aagcctgaat accaataata caaccggcgg agaactcatt tttaatttag gaagtatttc
    15421 agaagatgtc cttaaggatg ggaaacacgc gtttgaaaac gggctcccgg ctgatgggtc
    15481 attaaacaat gtcacccaaa acgtctgggg tcgcgtgaca gacgccactt acctcaatga
    15541 tgcttttgat aattctgccg gcgccagggt gaaccaggat gtcggactag acggattgaa
    15601 aaatgcggat gaacaagcgt tatatcccga cttcagtgcg ttagctgacc cggctgctga
    15661 tgattttcgt cattaccttg acccttcatt cgatcagttg gatgcaaaaa ttctggaaag
    15721 gtataaagac tttaatggcc ttgaaggaaa tactcccctt agctccacgt tttcaaacag
    15781 tcaattcccc gataatgaag acctggattc tgacaatacc ctgaatgacc tggaggaata
    15841 ttatgagtac tctgtttctt tgaatccggg ccagcttacc atagggagta aatatattgt
    15901 ggatgcggta accagtaatg ttgatttgcc aaatggctcc agggaaacag ttacctggta
    15961 tctcttccgg attcccgttc ggaattttga tagaaaaatc ggtaacattc agggatttaa
    16021 gtcaatccgc tttgttcgta catatttaac gggctgggag cagcctgttg ttttgcgaat
    16081 gtcaaaattc agacttatag gcagtcaatg gcgtagatat accaggagcc tggaagaaaa
    16141 ccggtttgac caacttcctg aaacggactt taccgacttt acggtatctg tagttaatat
    16201 tgaagagaat agccagggcg gtggagtaaa ttcaccctat accctgcccc cgggtttcag
    16261 gagagatcgg gataatactt cagtgattga acgaaatctt aatgagcaat ccatgcggct
    16321 ttgtgtggaa gacctcagtg ataaagatgc tcgggcagtc tttaaaaatg ttgacataga
    16381 cctgataaac tatgggagta taaaaatgtt tatacatgct gaaagtgaca atgccgtgga
    16441 tgatgaaata actggtttct taagattggg cactgatttc accgaaaatt attatgaaat
    16501 tgaagttcca cttaagatta ctcctgcaga tgtaagtacg aatgacccta atatccaacg
    16561 gctggtatgg ccgctcgaaa atgagataaa tattgatctg gacctgttat accaattaaa
    16621 agctcgacga aacagaacca ggaatccgga tgaatttggc ttccgctatt cagaaccata
    16681 tactgacggc ttgcggaact ataacataac catcgtggga agacccgaac tcagtaccat
    16741 acttgggctg atgatcggag tgagaaatcc tggctcacct gatagatctt ccaaatccgt
    16801 ttgtatttgg gccaatgagt tgcgggtgtc tgattttgat cgtaaagcag gatgggcagc
    16861 aaatgctaat gtgaacgtaa agcttgctga tctcggtaca attagcggct caacccgcta
    16921 cacaacacct ggatttggag gcgtccagac caaaatttct gagcgagagc gcaaggaaac
    16981 tctggaatgg gacattgcca ctaacctggc ccttgataag ttgctgccca gaggtacagg
    17041 tcttaaaatt ccaatgtatg caagttacca gcaaagtgtt attacgccgg agttcgaccc
    17101 aaaagacccg gatattccac ttgagcaaac actggctact ataagcaaca ccgaagaaga
    17161 acgaaattac aagaacattg ttcaggataa aggcacgcgc aggagcctta attttacggg
    17221 gatacgaaaa acccgtaata atcccgaatc aagaagcgat ttttgggata ttgaaaattt
    17281 ctcctttaca tatgcttata gcgatttcaa acgtagtgat ttcaatattg aatcctacct
    17341 gctgaaaaat cagcgcggct caattgatta taactatgcc tacgatccgc tctatattga
    17401 gccctttaaa aatgtcaaat tcctgaattc cccacattta aaacttctta aggatatcaa
    17461 cctgaatctt cttcctaata gcttcggctt acgaggtgaa cttgatcgac gcttcataaa
    17521 aacacaatat aggaatgaga atttggatat tggcagcatt gagccaacct atgaaaagag
    17581 tttcttcttt aaccgtaatt atactctcgg ttggaatctg actaaggcca ttagcttaaa
    17641 ttacacagct cgtactaatg ccataataga tgaacccgaa ggagacattg agacccagga
    17701 agaaaaagat gaaatctggg ataatattct ggatttggga cgaacaaaaa cctttaccca
    17761 aaatgttaat gcttcctata aacttccatt tgataaactt cccctgattg attgggttag
    17821 tgccgattat caatatacag ctggatatat ctggactgcc ggttctgtaa acctccgcga
    17881 ttcactgggt aacattatta gaaatgacag aactaataat gtatccggta aacttgattt
    17941 cctgaaactt tataacaaga ataagttttt gaaggatata aacagccccg ttaaacgccc
    18001 ggttgaccca aaagcggata ccgtgcaaag acccaacttc ggagccttga aaggcgtgtt
    18061 gcggttaatc atgtctctca gaagtataaa catgacgtac agtcttaatg agtcaaccat
    18121 cctgcctggt tttcttccgg aaccctccta ttttggccag gatgatggtt taagtgctcc
    18181 cggttggggt tttgtttttg gaagtcagga taacgaaatt aaaagaagag cggtggaaaa
    18241 taactggctg gcccagggga ccgggctcac aaatcctttc actcaacggc aaacgattga
    18301 cattggactt cgcgcaaccc tcgaaccttt taatgatttt aaaattcagc tcgatgccaa
    18361 gaaaaccaag tcctccactt ttcaggagat tttcagatac gatgaagatt cattgacctt
    18421 caggtcattg aaccccacac gttcaggaac tttttcaatt tcttatatct caatcaatac
    18481 cgcttttaag aaagaccggt cagataacag ttctccgctg tttgatgatt atatcgcata
    18541 cagggaggtg atcagacaac gactgaatga tttgaatagc tccggtgaat ataatctcaa
    18601 ttcacaagat gttcttattc cggcatttat tgcagcatat agtggaaaga gtgccgaaga
    18661 aataaagctc agcccgttcc ccggtactcc ccttccaaac tggagagttg actacaacgg
    18721 cctttcaaaa gttgaagcct taaaagatat tttttcatcg gtaactctta cacatgcgta
    18781 taattcaaca tattcaatta atagttttac taatagcctt ttgtatgaag aaggagttgg
    18841 attggacaaa gacattgaag attatcctct tgcatcaatt gtaaatgaga atggagagct
    18901 tgtaccaatt tatattatca accaggtagt aattacagaa agatttagcc ctttgattgg
    18961 acttaacgtt agaaccagga gcaggctcac cgcgaaaatt gattataaga ttgaacggac
    19021 aatgacgcta aatttgtcca atacccagat cacggaaatt aaatccaatg acattggctt
    19081 tgatttcggt tacacaacct cgaatttcac cctccctttc agggttcagg gaagaacggt
    19141 tgcccttaaa aatgatttga cattccgaat gaacttctca gttcgcgata ccagaactca
    19201 ccaaagaaga attgaagaag gcagcacaat aaccaatgga aatgtcaatt tccagctccg
    19261 acctaatatt aattatgttt tgaatcagcg cctgagctta caagtttatt ttgaaaggaa
    19321 tataaatgaa cccaaagtaa ctaattcttt caagcgggca accactgcat tcggagtcca
    19381 gattcgattc agtttagcac aataaaattt ttgcaattga aatttccgtg tatttttggt
    19441 gcaaaaaaca agcgacatga attttcctga agaactgaaa tatacaaaag accacgaatg
    19501 gattaagatt gaaggtgatg aagcgacagt tggaattacc gatttcgctc agagtgaact
    19561 gggcgatatc gtttatgttg aaattgaaac tgccggcaaa tctttatccc agggagatgt
    19621 ttttggaaca gttgaggccg ttaaaacggt gtctgatttg ttcatgccgg tttctggaga
    19681 agtggcccag gtgaatgctg atttgcaaaa caagccggaa atcgtaaaca acgatccata
    19741 tgggaaagga tggatggtta agctgaagat ttccaattcg aatgaaacaa agggtttact
    19801 ctcagccgcg cagtataagg aagtaattgg ccaataattt ttgattaata aatactctat
    19861 agccagcctt gtctgggcaa tttttacagg gactttgacc tcacttcccg gcactttgat
    19921 cccccggatt acgggaacag ccaattttat tctggacaat gtgagtcata ttcttttctt
    19981 ccttatcctg ggttttctgg taggaaagag ccttttattc caatttccat cttttggtcc
    20041 tcaaaaagct tttttcctga cattttttat ctgcattggc tatggttttg cccttgaatt
    20101 agtccaaata ccgattccgg agagagtttt tgaatggagc gatgtttttg caaatgggtt
    20161 tggagcaatt tttggagcat ctataagtgt tttattgaag aaagcgaatt aaaataacct
    20221 gggaatttta ttatatttgt tagaactgct tttctaaatt ttctaaaaaa tagcatatgg
    20281 aagcgaagaa aaacccaaaa gctgacctta caagaacatc cggtttattc ctgaatgttg
    20341 ggctggtaat cagtctattg ttggtgatct ctgcctttga gtatagattc tatgacgatg
    20401 gaagcctggt taacctgggt caggtgaaag atgaatttga ggatatgttg gaaattcctc
    20461 cgacggaaca gccgccgcct cctcctccga aaattcagca gccagaaatc attgaagttc
    20521 ctgatgaaga agagatcgaa gaggaaattg aagtagatct tgatgttgag attactgaag
    20581 aaacggtaat tgagaatatt attattgacg aagcccctga tgaagaagta gctgatgata
    20641 tttttaacat tgttgaggat cagccggatt ttccgggagg taagtcagca ttttacgatt
    20701 atgtggccaa aaatatgaag tatccaacaa ctgcccgacg gatgggtact gaaggaaggg
    20761 tgtatgttca gtttgtagtt gataagtcgg ggaacattac acaggtagaa gttatcagag
    20821 gcattggagc cggttgtgat gaagaggctg aacgtgttgt aaaagcatca ccaaaatgga
    20881 aaccaggtaa gcagcgtgga caaccggtga gagtaaggat ggttcttcct ataaccttca
    20941 aactaaattg attctaatag aatttgaatg aacccggcaa gaccgggttt ttttattccc
    21001 ctctttttca gtcttgtttt caaatttaga catttctttc ccctatccct tccccggtat
    21061 tttttgctga aattcattga aattgggctg atcattgtgc tattgagtat ctcatttttc
    21121 aatgattaaa caaaataacc tatggaagcg aggaaaaacc cgaaagcaga ccttaacaaa
    21181 accacagggc tgtttctgaa cgttggcctt gttcttagtt tgctaatggt gatttcagcc
    21241 tttgaatgga gattttatga tgatggcagc ctggttgatt tgggtcaggt gaaagatgag
    21301 tttgaagata tgttggagat tcctcccacc gagcagcctt ctccgcctct aactttccaa
    21361 aagttccaaa cttttggaaa gtttacggaa tcaacctcta tacaacattc gcaagttgtt
    21421 ccttgatttt ttccaactca tccttcattc ccacaacaag catttgtata tcagggtcat
    21481 tggcttttga cccaatggta ttaatctctc tcccaatttc ctgagaaata aagccaagtt
    21541 tcttaccggc attatcttct ttttcaagaa cttcaataaa atagccaagg tgatttccaa
    21601 gtcttacctt ttcttctgaa atatcaatct tttcgatgta atagataatt tcctgttcca
    21661 gtcgattcac atccgctctg tcattcccaa tcaactcctg aagattaccg gttaatctat
    21721 ccttgatttt ctttatccga ccaggctcat actcatcaat ctttttaagc aagtcattaa
    21781 tggtattcac gtactccttg aatttggatt ccaaagcttt cccttcttgc aaccgaaatt
    21841 catcacaagc cttcagagca tccatcaaac aatcacgtga aatctgccat gcctgttctg
    21901 cttgctcgct ctttgtttca ttcatcataa catctggaga ctgaatagct atcctgaaca
    21961 agtcatcatg tggcgcgatc acactgtctg ccaacctgcg caactgctca tagtattgtt
    22021 taaaaagttc tttattgatt gccacagaag tgatggggtc attttttgcc cgaatttcaa
    22081 cctcaatagt tactttgccg cgaataagtt tttcagttat gatatttcta atttccatct
    22141 ccttcccgtt aatcgctttt ggaagcctga tgctaacatc aagaaacctg gagttaagag
    22201 ttctgatttc aactgttgat tcgaaatttg ggttttcctt tgtacaggtg ccgaaccctg
    22261 tcattgaact tatcattcag taatattttt tgacaagtta aagatattta tgcaaactga
    22321 aactgaaggg gattaaaaat caaaaccaaa ggatgcctgg aacttcgggg ttcctctttc
    22381 aaaattttca acaggccagg caagatcaaa tttcataaag aatccaagaa ttacagttcg
    22441 taatcctgcc ccaaaacttt gcagccatgg actccggtaa ttcttgattt cactccggaa
    22501 aattcctcgg tctataacca gggtatttac ttcattcttt tcgctaaaag ggttcggccc
    22561 tgtccacgaa gatccaatat cataaaatcc gataaattgc aggttcctga agaagtttga
    22621 tgaaattggt cctttataaa aatatttgat tacaggaaat cgaagttcag catttaatac
    22681 cagtgcatca tttccataaa aagtattata gctgaagcca cgcaaaggag ttacatattc
    22741 aagaaaaagc aaatcactgt tatcactgtt tcttgcagta tacaaagggc tgccctgatt
    22801 ctctgaatca ccccggttct tggtttttga aaataaccag ttatcaacac caccaagcag
    22861 gtaattttgg ggattccttc cgaaaaactt cccgtagaaa atgcgcgtag caaaaacaaa
    22921 ctctctggaa attttttggt aatgcctgaa atcgaaagtc agattactaa agcttttgtc
    22981 tttatctgtt atcccttccc agtgtttaaa tgtaattttt accctggtgc cttcaataat
    23041 gttcattccc cttgatatgc tattatcata taccagttca ctgtttatgc ccacataatt
    23101 tctgtttatg gttgcggccc ttcccggtgg tatctgccca cctaacaagg caaagtcaag
    23161 atcttcaaat ttagtatttc caaaaaacgg cttcagcgta aacctggaac gaactgatag
    23221 cggatagctg gcgcttaatt caataatatt cttcttgtat ttttgaggca cgtcctccgc
    23281 cgttggccat tccattaccc ttctgtagaa tctgaaacca tagtctatca ttccgcgcaa
    23341 aaatttatac tcgccaacaa aatcgccgct tctaaagtcg gttgtcccca ttacacttcc
    23401 ggtaaaacta tggtcttcca ggatgtcttt catttgagtc tcaatcagaa ttccaaatcc
    23461 tatcatgggg tcaatcaccc aggaggttac cagattatca gcactaaacc gggtttcata
    23521 atcaagcgga ccaaatatgg ttttctgagt ttgaaatctt ctgtactgac ttaaaaaaga
    23581 ttcaggctga tcaattctcg ccttggattc caattcctta tcaaaaacat aattatctgt
    23641 atctataata tcctctttag cttttgattg ctgaactgaa tccggcttag aaaaatctac
    23701 ctctaccttg ttgctccggt ttaccaggtt ttgtctcctc ctgtcggtaa taaatctggc
    23761 ttgttttaca gattgccttt tagtttgaga agtgaaaatg ctacggttac gatcaaaatt
    23821 gtaatcgata tatatattgg aaacattttc gttttccatt acaaacgccg ctcttcctaa
    23881 attaaaatta aggtcataat accttatatt attactgaaa ttgctgactt ggctgtaaat
    23941 ggaatctatc agattataat tgaatacatt ttgaattcct ttctgatcag acaggtaata
    24001 aaagtcagaa taatttaagg ccaatggact tacatcctga ctaaatgtat tggttacgcg
    24061 gttcagggtt aggttagtcg tatcaagatt gtaaaggaac aaattaaaat tatcacccaa
    24121 atctttatac gactcagaca caacgcttaa ggtgtcattt gcccggtttg aactgaatag
    24181 aataatattg ctatttggaa tgaagaccgg attaatttca tcgtacaagt cattggttaa
    24241 cctcttaatt ctgcttcgtg ttactgaaac cagatacaga tcatttattc cggccacatc
    24301 ggcactcatt attgccagcc ttccatttcc tgaaaaatta aaagaatgaa ctttttcaaa
    24361 ccgttcaaat cttgtaggaa ttcttgtgcg cgtggttatg tcatacaacc acaatgtggt
    24421 tctggccttt tcataattta ctacacccag tgtagtgtta tccgcccacc ctagcaatgg
    24481 agactggtaa tctactttct gattgataac tttataacct cccgacagga ctttatcttc
    24541 gttctgagta tctaaatctc ttatccatac agaatatctg ccattgtagt ttctaacata
    24601 ggccagtttc gttgcatccg gacttaattt tacctggctg taatcaaatc cttttctatt
    24661 tttctctaaa acctgaagcg catctgcagg atcaacataa tcggttgaaa caatttgttc
    24721 tatttcgata taaaagtctt gccaatcatt gaggaattga cggaacgaaa ggcccaacgt
    24781 gttggcaatg cttttttcct cattccgaat tatccgggtt aggtttagaa tgttcgaaat
    24841 ggaactcctt ccgtaagtct cagctatata attccaaatg gattggccag ccagtcttgc
    24901 ctcttcaccc gttagcctgg ataatcttag acgtcgctga gcggtaacga aatcacgaat
    24961 gtaatcatcc atttccaggc tccagccatt ggctatgtag gcagaagctc cctccacaaa
    25021 ccaagtgggt aaatggagga gataagcgct ttggaacata tcggacagac ttccaccaaa
    25081 catcatatca ttgatcaaga gcctgctggt gttgtaaatg agttcctgac ggaaatcatt
    25141 taccgtgccc gggtgagcaa tttctacata ggattttatg aaattcgttt gaccaccttc
    25201 cgagtaatca agatcattaa tgcccacatt gctttgctgg aggtcagcaa ttgaattgta
    25261 aagaaacact ttggtttttg tataaggact gtagccaata acatcggtta tcctgtcaaa
    25321 ttcaccttcc aaaaaattga tcgcttcctt agcagcaaca tccccaccct cgtaaaaata
    25381 tacatcgaaa ttatcggatg aataaaattt ccaatcaaag actttatact gaaccctgtt
    25441 tttcccaaac ttttccctcg cctgctgggc ataaagcgga ttcaaaattg tgaaaaaaca
    25501 aagaatgatt acgcatatag caacacgccc ttccctcatg cagaaatttg ttttggaacc
    25561 tgaaatttaa tcaaaaaaac gttttatatc tatctctcta tcaagttctt tgccattctc
    25621 aaacttatcc gctaaaagtt tagaaatgta cggcgctaag gaaactccct tggtgcccag
    25681 tccatttaat atgcccaatt tcttattgtg aggatgttcg ccggcaaccg gccgacggtc
    25741 tactgaacta ggcctgaatc ctgcaatttg atttcttatc cttatttcag gaagaaagaa
    25801 ggaatggatt ctttccagca gaatctccct tgccttaatg gtcgtctcca tggtttcatc
    25861 attaaaatca taagttgctc ccaccttaat caggccatca ctttgtggca aaataaaggc
    25921 aggaccgtta aagatcattt ctggtaattg aatgctggtt tcgacttcca gcaattctcc
    25981 tttcaccgcg ttaaaaggtt tatcaccaaa tggagttttt ctcacgcctt cataacctaa
    26041 acataaaatt agccggtctg cagtagatcc atttaccgcg aatggagact gccagttgat
    26101 gttatcaaga gttacttttt cctgacggta gatccccaat tccataaaat aatttttgag
    26161 cgccttcagg aatattgaaa cattgagata cccggaattg ttaagaagaa ttccaccgta
    26221 actatctttt atgaattcag aataattgcc cggcctggta atctcctgaa caaaagccgg
    26281 aacttcagca gctggtaaac ttaaccaatg attctgttcc tgcatatttt ggaaaaacct
    26341 gaaaacaggc atctcatgga aaaattttgc attgacgagc ttttcaagct ccttatagaa
    26401 ttttggaaaa caggcaaaca cctcctgggc tttccatgtt gtaacccacc tccttccggt
    26461 gatgggattg aaaatacctg cagcagccat tgaagatgaa tgcaaatcgg gatcattaaa
    26521 aataagaacg gtcttcttcc ttctcatcaa ctcaactgcc aaagctgtcc ctgccaagcc
    26581 ctggccaacg attatatagt catataccgt caagttcaaa tttgaaatat cttaagaata
    26641 cagcaacttt gtatcgcgaa taaagctacc atgattaaaa ttaaaaaatt tgtctttaat
    26701 ccgttttcag aaaatactta tgtgcttttt gatgaaacgc gggaagcagt gattattgac
    26761 ccgggatgtt caactcctgg ggaagaaaga gaattgaaaa gttacgttga aaagtatcaa
    26821 ctgaaggtga tagctgtttt gaatactcat ggtcacatcg accatgttct gggtaaccaa
    26881 tttgttgcag acacatggaa tgtgaagctt gggatgtgta agagagatct tcctacctat
    26941 aaatcagtaa tctcctacgc atcaaactat ggatttccaa aataccggga aatggagcct
    27001 ggattttttc tggatgaaga agatgaattt gaatttggag tgagtacact cagcgtaatc
    27061 tttacaccgg gacattcagt tgggcatatc gtttttgtga accaggaaca acagtttgtt
    27121 attggcggcg atgtgttgtt cagcggaagt attggaagga cagaccttcc cggcggaaat
    27181 tttgacactc tgattgaaag tattcaggac aagttatttt ccctgcacga cagttttgtc
    27241 gtatattcag gtcatggacc tgaaaccacc atcggtgaag aaaaggtcag caatcctttt
    27301 tgccgaattg aacaagagta gaatcaccat ttgaaaaatc tgccgcatat tctcacttta
    27361 tttaacctgt tgagtggatg ccttgggatc acttttcttt ttcagggcca tttaagtctt
    27421 ggggccataa tgatctttgc cggcgctatc tttgatttcc ttgatggatt tgcggcccgg
    27481 atgttaaatg caaagagtga gctgggaaaa caattagatt ctctggctga tttggttaca
    27541 tttggcgttt tgcccgctct gatactttat acattatcag cgaatctcaa cgatggttgg
    27601 atttcctatc ttccgttagg agttgcaatt gcggccgcct tacgcctggc caaattcaat
    27661 atcgatgacc ggcaaacaaa tttctttatt ggtttgccta cgccagctgc agcgctgctg
    27721 gttgcttcaa ttccatttat tatggatgaa gaacatgtta ccggaacatt gaatatttat
    27781 agtctgatag gattaagtgt cttcctttct attatgatga tatcccgggt taagctgatg
    27841 tcatttaaat tttcgcatta taactggaaa gaaaatgaac caaaatatat tctgattcta
    27901 ttgttcctgg tgcttgtctt ttatttctca ttcatttcca tacccctgat atttttgctc
    27961 tatttaatct tttcagtttt tgtgaaatat ccagaaacca caaaaaaagg ctgatttgaa
    28021 tcttttaggc aatatttttg catttaaacc gttgactttt cactaattat ccggatatat
    28081 ttctggcttt cagtaatcct atgttccatc ctcagactca aaagtatttc acttgtcttc
    28141 tgcctgccct attattgatc tttaccaaca ccggatactc gtttcagcca agcttatcta
    28201 actctattct cagctccggg aaatggatta agatagaggt gagcgagcgc ggcatttaca
    28261 gaattacaaa acaagatttg ctcaattccg gctttagctc tgccgggatc gttccatcaa
    28321 aaatcagact ttttggtaat ggaggaaaag ttattcccca aaaaataagt gatccgagac
    28381 cagaagacct catagaaaat gcgatttata ttaccggaag taacgacgga acttttgaca
    28441 cgagtgatgt aatacttttt tttgccgatg gaccggatga attgcgatac gatcagagta
    28501 ataagctgca gtattccaga aacccatatt caaattcctc ttattacttc cttacaattt
    28561 ccgataatga tggcaaaaga attgaatcca ggattaatga aggcggttct tttactctta
    28621 ttgattatta tcacgatctt aaatcgcacg aagtagatga acataatata ttacaagccc
    28681 ttataacttc atatggtggt tccggacggg aatggtatgg agaaagattt aaggaaaatc
    28741 ttaatcaatc catctctttt aatatagata acttatttga tgcgggtaat tctacactca
    28801 tcacttctct tttgggcgtt tcgaagacct catcatcatt ccagacaaaa ataaataata
    28861 ttcttctggg cgatcaacca attactgcca tacctgattc aagatatggg gtaaagggac
    28921 aagctcgggt tgacactttt ttgatttcca attcactatt gggtcagatc caaaatgcgg
    28981 aaattcaatt acagtatttt cctggtaatg caatcgagga tgccggatat ttaaactacc
    29041 tcatcttggc ggctgaaaga aaattaatta agggagccgg gtcactcata tttcagaacc
    29101 tgttaaccaa tgtttcaggg agtcatactt atgcgataca aaatttacaa acccccgata
    29161 ttatttggga tataacaaat cccaaaagtc ctgtcctgca agagtttgat ttcaccggcg
    29221 atacaggcat gtttggaata acaacagaca gtttgcaccg gttcattgca ttcaattca
//
LOCUS       DASWHX010000214        10693 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_1738913, whole genome shotgun sequence.
ACCESSION   DASWHX010000214 DASWHX010000000
VERSION     DASWHX010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 10693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10693
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1738913"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..188
                     /locus_tag="VGA21_14050"
     CDS             <1..188
                     /locus_tag="VGA21_14050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651681.1"
                     /translation="SGMDKSRDLKFDELQMKLRPEIVTNGEFQSAGKDQEKKDNVKLN
                     GVGGDIKPNPDNNRKNI"
     gene            201..878
                     /locus_tag="VGA21_14055"
     CDS             201..878
                     /locus_tag="VGA21_14055"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEX9651682.1"
                     /translation="MSSLSFNLLSLMAIIIIILLSLSFWMKSSKKLSTEKRNRNFITI
                     RITVFLIGFVLAIISVERGDSASHGLKIPDLIAWIVTVIMMMVFVIAMSRGAKNKSIQ
                     KVVPYLAYNHWNYSRIGINTVTWVLYLAGYEYLLRGILLFESVDNFGVINAVAINVVI
                     YVLIHTPKGWLETIGSIPMGFLLCALTIGSESVIPAILVHASMSVSIDFFSIMNKQQM
                     RKLRVKS"
     gene            974..2059
                     /locus_tag="VGA21_14060"
     CDS             974..2059
                     /locus_tag="VGA21_14060"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HEX9651683.1"
                     /translation="MLTRKQFLKRMAAISAATTLSGFYGFQCTPRKVKEVVEGNDMIT
                     FDLHSHPGQFFWKGIEGFKGDEGVVKTINEMNEGGLNGGFISMVSDMLVLKRTETGIV
                     PERKFEPGEAWHDIKRQLSVINELLQTLDAHQGLKASDLITDPSSNVAIYLACEGGDF
                     IGEDIERVDQIYESGIRSIQLVHYAPNNIGDLQTHDAIHQGLSEMGREVVRRMNSLGM
                     VIDVAHASFETVKNIAEVTEHPVILSHSILKMEAERPISARAITAEHAKAVAETGGVI
                     GAWPSGFNRNFDEYVENILRLIDVVGIDHVGLGTDMDSNFRPVMDSYLQLPDWIAALK
                     WKGLSEEETRKVAGGNALRVLQGVLKS"
     gene            2563..2997
                     /locus_tag="VGA21_14065"
     CDS             2563..2997
                     /locus_tag="VGA21_14065"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEX9651684.1"
                     /translation="MFSKSCQYALQSILYIVLHNKDARLVGLNEISESQKIPRHFLSK
                     ILQQLVKAKVLNSAKGPNGGFSMNKSPEKLNLMTVVKIVDGFEIFDRCGIGLKTCSDK
                     NPCPIHFNYKEVKQRIKDLLSEKTLSELGEDVLKGKSIVSYR"
     gene            3091..3594
                     /locus_tag="VGA21_14070"
     CDS             3091..3594
                     /locus_tag="VGA21_14070"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEX9651685.1"
                     /translation="MFPKENINLENKGIIGARESVTLISHVVLSEDTNSLHILLGGRL
                     MEWMDLASEITAQKHSQCIALTARIQEINFYKKIGVGDIVTIKAQITRVFNSSMEILV
                     EVFTEHVPELETIKTSDGLFTMVAVDLNGKPIRIRDIQPETEKEIRLFKEAGERKKQS
                     LKKKLNK"
     gene            3591..6191
                     /locus_tag="VGA21_14075"
     CDS             3591..6191
                     /locus_tag="VGA21_14075"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020527549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HEX9651686.1"
                     /translation="MIGITLEIEFDKTFVEKTSTQSKSSPKQSFSYEEVKERTAEYFG
                     GDALAAEVWINKYALKDFRGNIYERDPSDMHRRISGELSRIENEYPNPIGEEEIFQLL
                     DHFRYLVPQGGPMAGIGNDKQVVSLSNCFVIGNPGKSDSYSGIMRIDEEQVQLMKRRG
                     GVGHDLSHIRPRGSSVKNSAVTSTGIVTFMERYSNSTREVAQDGRRGALMLTLSSRHP
                     DILDFIHAKKDNAKVTGANISVKIDDEFMEAVQNDQHYYLQFPVDDNNHLVSKKVKAR
                     VIWDAIVDSGWSSAEPGILFWDTIIRESVADCYAKDGFKSISTNPCGEIPLCPYDSCR
                     LLAINLLSYVKNPFTPDAEFDFNLFDKHAGMAQRIMDDIIDLEIEKIDKIIKKIISDP
                     EPFDLKRVELNLWKKIKNKCQEGRRMGIGITAEGDMVAAMGLKYGSQEAVNLSEKVHK
                     FLALGVYRASVKLAGERGAFPLFDFSKEIKNPFIKRLAAEDPDLYKEMKKKGRRNISL
                     LTIAPTGTTSLMTQTTSGIEPAFSISYKRRRKLSDKEQDKNADFVDELGDRWSETYVF
                     HHGFKSWLLVNGYNPETVMHFDNDKLDAIIAKSPYSNSTAKDIDWIQKVKMQGAIQKW
                     VDHSISVTVNVPKETTREQISEIYLKAWENGCKGVTIYREGSRSGVLVRKDDKKDLYD
                     IKENHAPRRPKILKAKILRFLNNEEKWIAIVGMLNDKPYEIFTGKAEDSFAILSTVNE
                     GWVIKNKLKDSQTRYDFQYADKAGYRITIEGLSRCFDPEYWNYAKLISGVLRHGMPLK
                     FVVDLVDNLSLEGASLNTWKNGVSRALGKFIPDGTTPINQGCPECEQESLVYQEGCIT
                     CKNCGYSKCK"
     gene            complement(6203..7588)
                     /locus_tag="VGA21_14080"
     CDS             complement(6203..7588)
                     /locus_tag="VGA21_14080"
                     /EC_number="4.1.99.2"
                     /inference="COORDINATES: protein motif:HMM:NF009709.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050371 - tyrosine phenol-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006570 - tyrosine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine phenol-lyase"
                     /protein_id="HEX9651687.1"
                     /translation="MVLVKHKSWAEPYKIKVVEPLFITTREFRAKVIAEAGYNTFLLR
                     SEDVYIDLLTDSGTSAMSDRQMAGLMLGDEAYAGSRNFYHMEDAIRKYYGYKYVVPTH
                     QGRGAEHIISSILIKAGDFIPGNMYFTTTKLHQELAGGVFVDVIVDEAHDPNSLFPFK
                     GNVDLNKLESVIKKEGANKIPYVAIATTVNMAGGQPISLENLRAVRELTSRYGIRIIH
                     DMTRVAENAFMIKMREEPFKDWSVAEIVKEICSLTDGATMSAKKDALVSIGGFLAVND
                     EFVFEEARNMVVIYEGLHTYGGMAGRDMEAMAIGIEESVQFDHMHARIGQVHYLGQKL
                     IEFGIPIVRPLGTHGVFLNAKEFLPHIPQENFPAQTLAAEIYIDSGVRSMERGIVSAG
                     RDSTTGEHRFPELELVRLTIPRRVYTQAHMDVVAESVQSVFETRQGINGLKMVYEPKH
                     LRFFQARFEKK"
     gene            7741..7956
                     /locus_tag="VGA21_14085"
     CDS             7741..7956
                     /locus_tag="VGA21_14085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651688.1"
                     /translation="MKKFKIKSKSHVPWVRDHLTDQPYLEIEIFDEEVLFDILKIDPV
                     AKELKSECIFVSKEATRKLGKWILDNC"
     gene            7994..10000
                     /locus_tag="VGA21_14090"
     CDS             7994..10000
                     /locus_tag="VGA21_14090"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX9651689.1"
                     /translation="MKKFFLIIVLNYLIFPTFLQAQDYDLGANINTDKVEYAPSISAD
                     GNTLIFQSNRDGNYKLYFSERETSGKWSLPKPIINVNKFAEKAGLIGGPSISYDGNYI
                     YFFANLDGGVGVEDIYYTERKGNDWSEPVNVGSPVNSRNYEGFPSISSDGKKLYFMRF
                     SGKQLYEGKFCYQLWVSERDSDGKWGRPLPLPAPVNQGCEKCPRIMSDNETLIFSAIR
                     GPSTKNNNFDLYMSRLDGVRWTEPVPLDFANTPDDEAFGSVPSSGDIMYLNVDNNGNQ
                     DLKGVPIPQALQPKKVINVKGKVTDAGTGKPIGANIIIIRDLDTLNTGALQSNPYDGN
                     FTYVLTEGYAYEVIITAPGFNAQTLNYDLKELQDYKLINEVVNLTPYRGLLRLTIVNS
                     LNKDTITTAVVTTLSNPVEGINGYYEIPVSFGIEYKIEMNAKGFEPGQMDFQVSDIMP
                     KKVYKKSIELQPALPELIIVVNDKESGEKLKVYLEIFDKTKRTLKYKGMMEVGDYSGN
                     LEHGHDYIMRITRPGYFFFEEGINLSEIFYGLKIEKSIVMTPLKKGEKLTLNNIIFEL
                     NSADLTQDSKTELDNVFELLKQNRNIVLEIAAHTDDLGDDTYNLQLSNARAKSVKEYL
                     LSLGVPEMMLVSKGYGESMPAVRNDSEENRKLNRRVEFLVLDAK"
     gene            10380..>10693
                     /locus_tag="VGA21_14095"
     CDS             10380..>10693
                     /locus_tag="VGA21_14095"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX9651690.1"
                     /translation="MSPNFKFNLLAITIFLFVLHPVSSQELVRVTPEEVGMSSERLGR
                     VTGVFNEYVEAGKIAGSVILVMRKGKVAYFEAFGYRNVEDKLKMERNTIFRIASQTKA
                     IT"
BASE COUNT         3415 a         1912 c         2287 g         3079 t
ORIGIN      
        1 gttccggaat ggataaatca cgggatttaa aatttgatga actacaaatg aaactgaggc
       61 cggaaatagt tacaaacggc gaatttcaat ccgcaggaaa agatcaggaa aagaaagata
      121 atgtaaagtt gaatggtgtg ggtggcgaca tcaagccaaa tcccgataat aaccgtaaga
      181 atatttagca gataaatgtc atgagctccc tttcatttaa ccttttgtct ttaatggcga
      241 ttatcattat tatcttatta tctctctcgt tttggatgaa gagctccaaa aaactttcta
      301 cggaaaaaag aaaccgcaat ttcattacaa taagaattac ggtttttcta attggttttg
      361 ttcttgccat tattagcgtg gaaagaggag actcagcttc tcatggactt aaaattccgg
      421 atttaatagc gtggattgtt acagtaataa tgatgatggt ttttgttatt gcaatgtcgc
      481 gcggtgccaa aaataaatca atccaaaaag tggttccata tttagcatat aatcactgga
      541 attacagccg gataggtata aatactgtga catgggtatt atacctggca gggtatgaat
      601 atctgctccg aggtattttg ttgtttgagt ctgttgacaa tttcggggta ataaatgctg
      661 tggcaattaa tgtagtgatt tatgttctta ttcacactcc aaagggctgg ttggagacta
      721 taggttctat acccatgggt tttctgcttt gtgctttaac catcggttcg gagtcggtaa
      781 tcccggcgat actggtccac gcttccatgt cggtttctat tgattttttt agcattatga
      841 acaagcagca gatgaggaaa ttgagagtta aatcctaatt aggtttgaaa atggtgaaaa
      901 aagagacagg tttgaatcag taacagttga atctattata gcaaattcaa ttcgattcca
      961 aaccaaagaa cttatgctaa ccagaaaaca gtttcttaaa aggatggctg caatcagtgc
     1021 tgccaccacc cttagtggat tttatggttt tcagtgtact ccacgaaaag ttaaagaagt
     1081 ggttgaagga aatgacatga tcacttttga tttgcacagt catcccgggc aatttttctg
     1141 gaaggggatt gaagggttta agggagatga gggagtggtg aaaacaatta atgagatgaa
     1201 tgaaggtgga cttaatggtg gatttataag tatggtaagt gatatgttgg tgcttaagcg
     1261 aaccgaaacc ggaattgtgc cggaaaggaa atttgaacca ggggaagcct ggcacgatat
     1321 taaaagacaa ttgtctgtta tcaatgaact tctgcaaact cttgatgctc atcagggcct
     1381 aaaggcttct gatttgataa ctgacccatc ttcgaacgtt gctatttacc tggcatgtga
     1441 aggaggtgat tttattgggg aagatattga gagggtggat caaatttatg aatcagggat
     1501 tcgttctatc cagctggtgc attatgcgcc aaataatata ggagatttgc agacccacga
     1561 tgcaatccac caaggattat cagaaatggg tagggaagta gtgcgaagaa tgaatagtct
     1621 gggaatggta atagatgtgg cgcatgcttc ttttgaaacg gtgaagaaca tcgcggaagt
     1681 aacggaacat ccggtaatcc tttcacatag cattttgaaa atggaagcgg agcggccaat
     1741 ttctgctcgt gctataactg ctgaacatgc taaagcggtt gctgaaacag gaggagttat
     1801 tggcgcgtgg ccctctggct ttaataggaa ttttgatgag tacgttgaaa atatcttgcg
     1861 acttatcgat gttgtgggta tagaccatgt cgggcttgga acggatatgg atagcaattt
     1921 ccgacctgtg atggacagtt atttgcagtt acctgattgg atagcggcac tgaaatggaa
     1981 aggcttatcc gaagaggaaa cacgtaaagt tgcgggaggg aatgcccttc gggtacttca
     2041 gggagttttg aaaagttgat gcttgttgat atgaattgcc atgaacaggg gaatcagatg
     2101 acttaaatca ttttaattca ataatagttc aggtaactta atgcttatta aaggagtaag
     2161 tcatgaaaat attgttaatt attttcgcaa cattaattgg cattggactt tcgccggtag
     2221 cgtttttatt aatgcctttt ttggcattag caacgattat atgggggctg tcattaatga
     2281 ttagagaatc aagtcatttt caccgtcatt ttcaccatca ttctcactga attaattcaa
     2341 acttgcggtg aattgtctga ggaatgcagg ttcataaaca attttcattc ccttggttga
     2401 atttttgtta tccaaaacat ttgtgaaaat ttaattgatt gctaaatagc cttcagcaag
     2461 atagaaatga cgtatccatt gacaatggac attactgcga gcctcaacct tcggagatct
     2521 aaaaaagtaa tgcttatatt tcagctttag taaataaact ttatgttttc aaaatcctgc
     2581 cagtatgcat tacaatccat tttgtatatt gtacttcaca ataaagatgc ccggcttgta
     2641 ggcctgaatg aaatttcaga atcacaaaaa attcccaggc atttcctcag caagattctg
     2701 cagcaattgg ttaaggcaaa agtacttaat tccgctaagg gcccgaatgg aggattttca
     2761 atgaataaat caccggagaa actgaatctt atgacggtgg taaaaatagt ggatgggttt
     2821 gaaatctttg accgttgcgg aataggccta aagacttgtt ctgataagaa tccatgcccg
     2881 attcacttta attataaaga ggtaaagcaa agaataaaag acctgctttc tgaaaaaaca
     2941 ctttctgaac tcggagagga tgttttgaaa ggaaaatcca tagtttctta cagataagac
     3001 tcaaaaataa ctaagtcggc gtaaacaaag ataaaattag acttttaggt ctaaatatct
     3061 ttttatattt tagagaaatt ttacttcccc atgttcccaa aggagaacat taatcttgaa
     3121 aacaaaggca ttataggtgc cagggagtct gttaccctaa taagtcatgt tgttttgtcg
     3181 gaagatacta attcactcca tattctgctt ggcggcaggc taatggaatg gatggattta
     3241 gcttccgaaa ttactgcaca gaagcattct caatgtattg ctctgacagc acggattcag
     3301 gaaatcaatt tttacaagaa aataggagta ggtgacatag ttactataaa agctcagatt
     3361 acccgtgtat ttaattcctc aatggaaatt ctggtagaag tatttactga gcatgttccg
     3421 gagttggaaa caataaaaac ctctgatgga ttatttacaa tggtggctgt ggatttgaat
     3481 ggcaagccaa ttcgaatccg ggatattcag ccggaaaccg aaaaagaaat cagattattt
     3541 aaagaagcgg gagaaagaaa gaaacaatcc ttgaaaaaaa aattaaataa atgataggca
     3601 ttaccctgga aatagagttt gataaaactt ttgtagaaaa gacttcaaca caaagcaaat
     3661 caagtcccaa gcaatccttc tcctatgaag aagttaaaga aagaacagct gaatattttg
     3721 gtggtgatgc actggcagcc gaagtatgga taaataaata tgcgttaaaa gactttagag
     3781 gaaatattta tgagcgtgat ccttctgaca tgcacagaag gatttccgga gaattatcac
     3841 gaatagaaaa tgagtatcca aatccaattg gggaagaaga aatttttcaa ctgctggacc
     3901 acttcaggta tttagttcca cagggaggtc ccatggctgg tataggaaat gacaaacagg
     3961 ttgtctcact atctaattgc tttgtaattg gaaatcctgg gaaatcagat tcatactccg
     4021 gaataatgcg cattgatgaa gagcaggtac agctaatgaa acgaagaggc ggtgttggtc
     4081 atgacttatc gcacatccgg ccgcgtggaa gttcggtaaa aaacagtgcc gtaacctcaa
     4141 caggcattgt tacattcatg gaacgatact ccaacagtac gagggaagta gctcaggatg
     4201 gaaggcgggg agcattgatg ttaactttat catcaaggca tccggatata cttgatttta
     4261 ttcatgccaa gaaagacaat gccaaggtta ccggtgccaa tatctcagtt aagatcgatg
     4321 acgagtttat ggaagccgtt cagaatgatc agcattacta cttgcaattt ccagtggatg
     4381 ataataacca cctggttagt aagaaagtga aagccagggt gatttgggat gcaatcgtgg
     4441 acagcggatg gagttcagca gaaccaggca ttcttttctg ggatacaatt ataagagagt
     4501 cagtagccga ttgctatgcc aaggatgggt tcaaatccat atcaaccaat ccttgtggtg
     4561 aaattccatt atgtccttat gacagttgcc ggcttttagc cataaatctg ctcagctatg
     4621 tgaagaaccc gtttactcca gatgcagagt ttgatttcaa ccttttcgat aagcatgcgg
     4681 gaatggcgca gcgaatcatg gatgatatca ttgatcttga aatagaaaaa attgataaaa
     4741 taatcaaaaa aattattagt gatccggaac cgtttgattt aaaacgtgtt gaattaaatc
     4801 tttggaaaaa aattaagaat aaatgtcagg aaggaagaag aatggggatt ggtattaccg
     4861 cggaagggga tatggttgcg gccatggggc tgaaatatgg cagccaggaa gcagtcaatt
     4921 tatccgagaa agttcataaa tttctggcct tgggggttta tcgagcttca gtgaaactgg
     4981 ccggagaaag aggcgcattt ccattgtttg attttagcaa agaaatcaag aatcctttta
     5041 ttaaaagact cgctgctgaa gaccctgacc tatataagga aatgaagaaa aaagggagaa
     5101 gaaatatttc ccttttaacc attgcgccaa ccggaactac gagcctaatg actcaaacaa
     5161 cttccggaat tgaaccggcc ttcagcatta gttataaaag acgaagaaaa ctaagtgaca
     5221 aggagcagga caagaatgcg gactttgttg atgaactggg agatcgatgg tctgaaacgt
     5281 atgtattcca tcatggattt aaatcctggc ttttggtaaa cggatacaat ccggagactg
     5341 taatgcattt tgataatgat aaactggatg caataatagc caaatcaccc tattcaaatt
     5401 ctactgcaaa agatattgac tggatacaga aagtgaagat gcaaggggca attcaaaaat
     5461 gggtagacca ctcaataagc gttaccgtaa atgtacccaa ggaaacaacc agggaacaaa
     5521 taagtgaaat ttatctaaag gcatgggaaa atgggtgcaa aggagtaact atttacaggg
     5581 aaggttcccg ttctggagtc ctggttcgaa aagacgacaa aaaggatttg tatgatataa
     5641 aggaaaatca cgccccccgc aggcctaaaa tcctgaaggc aaaaatcctc aggtttttga
     5701 acaatgagga aaagtggatt gccattgtgg ggatgctaaa tgacaagcct tatgaaatct
     5761 ttaccggtaa agccgaagac tcatttgcaa ttctttctac tgttaatgaa ggctgggtaa
     5821 tcaaaaataa actcaaggat tctcaaaccc gctatgattt ccaatatgca gataaggctg
     5881 gttacagaat aaccattgaa ggattgagcc ggtgttttga tcctgagtat tggaattatg
     5941 cgaaattgat ttccggagtg ctgaggcatg gcatgcctct taaattcgta gtagacctgg
     6001 tagataatct tagcctggaa ggtgcttcgc taaacacctg gaaaaacgga gtatcgcgtg
     6061 cgttgggcaa atttattcct gatggcacaa ctccaatcaa tcagggttgc cctgaatgtg
     6121 agcaagaatc acttgtttac caggaaggtt gcatcacctg caagaactgc gggtactcaa
     6181 aatgtaagta aaaaactctg gtctattttt tctcaaaacg tgcctgaaag aacctcaggt
     6241 gctttggctc atagaccatc ttcaatccat ttatgccttg ccgggtttca aatacagatt
     6301 gcaccgactc tgcaacaaca tccatgtgag cctgggtata aactctcctt gggatagtta
     6361 aacgaaccaa ctccaattcc ggaaatctat gttctcctgt cgttgaatct cttcctgcgc
     6421 taacaatacc tctctccata ctccttactc cactgtcgat gtaaatttca gcggcaagag
     6481 tttgtgccgg aaaattttcc tggggaatgt gaggaagaaa ttcttttgca ttcaaaaaca
     6541 caccatgagt tcccaaaggc ctcacaattg gaattccaaa ttcaataagt ttttgaccca
     6601 ggtaatgaac ctggccaatc ctggcatgca tatgatcaaa ctgtactgac tcttcgattc
     6661 ctatggccat ggcctccatg tcgcgacccg ccattccccc ataggtatgt agaccttcat
     6721 atatcaccac catatttctg gcttcctcaa aaacgaattc atcattaaca gccaaaaatc
     6781 caccgatact cacaagtgca tcctttttgg cactcatggt tgctccatca gtcagggaac
     6841 aaatttcttt aactatttct gcaacggacc aatctttaaa cggctcttct ctcattttaa
     6901 tcataaaagc attttcagcc acccgtgtca tgtcatgaat gatccggatt ccgtacctgc
     6961 tggttagttc tcttacagct ctaagatttt ccaaggagat aggttgccca ccggccatgt
     7021 tcacggtagt tgcaattgct acataaggaa ttttatttgc tccttctttc ttaatcacag
     7081 actcaagctt gttcagatca acgtttccct tgaagggaaa cagactattc gggtcgtgtg
     7141 cttcgtctac gataacatcc acaaacactc ctcccgctaa ttcctgatgt aattttgttg
     7201 tggtgaaata catattgcct ggaatgaaat ctcccgcttt tatgaggatg ctgcttataa
     7261 tatgttccgc tcccctgccc tggtgggttg gtaccacgta tttatatccg taatacttcc
     7321 taatggcatc ttccatatga taaaaatttc tacttcctgc ataggcttca tctcccaaca
     7381 ttaatccggc catttgccgg tcgctcatcg cagaagtacc actgtctgtc aggagatcaa
     7441 tatatacatc ttcagatctc aatagaaatg tattataccc agcttcagca atcacctttg
     7501 ctcgaaattc ccgggttgtg ataaataagg gttctactac tttgatttta tagggctcag
     7561 cccatgattt atgctttaca agtaccatag atataattgt tttaattcaa aggtggctta
     7621 aacggttgca aaatggcatg actaaaatca ggattataaa atcacctcaa tacatcctca
     7681 tgtgacatgt gtcagcgttt atataccaat tagtttccat attcgggtaa acttaaaaca
     7741 atgaagaagt ttaaaatcaa atccaaaagt cacgttccct gggtcagaga tcatttgact
     7801 gatcagcctt atcttgaaat agagatattt gatgaggaag tcttgtttga tattcttaaa
     7861 atagatccgg tagccaaaga actgaagagt gaatgtattt ttgtatcaaa agaagcaacc
     7921 agaaaactgg gcaaatggat attggataat tgttaatata agatacattt gtgctttgat
     7981 agtttaatta agtatgaaga aatttttcct aatcatagta cttaattacc tgatttttcc
     8041 cacttttctg caagctcaag attatgatct gggagccaat atcaatacgg ataaggttga
     8101 atacgccccc tccatcagtg ccgacggaaa tacactgatt tttcagtcaa accgcgatgg
     8161 aaattataag ctctattttt cagagcgtga aacctccggc aaatggtccc ttccaaagcc
     8221 catcattaac gttaataaat ttgctgaaaa agccgggcta attggcggcc cttctattag
     8281 ctacgatgga aattatatct atttctttgc aaacctggat ggtggagttg gtgttgagga
     8341 catttattat acagaaagaa aaggcaatga ctggagtgaa ccggttaatg ttgggtctcc
     8401 cgttaattcg cgtaactacg agggatttcc atctatctcc tcggatggca aaaaacttta
     8461 ctttatgcgt tttagtggca agcaattgta cgaaggaaaa ttttgctatc aattgtgggt
     8521 atcagaacgc gattctgatg gaaaatgggg taggccgttg ccattgccgg cacccgtaaa
     8581 tcaaggctgt gagaaatgtc cccggatcat gtcagataat gaaactctga tcttctcggc
     8641 tattcgagga ccctccacaa aaaacaataa cttcgattta tatatgtcaa ggcttgatgg
     8701 cgttcggtgg acggagcctg ttccactcga tttcgctaat actccggatg atgaagcatt
     8761 cggttctgtc ccatcttcag gagatatcat gtatctcaac gttgacaaca acggaaatca
     8821 ggatttgaaa ggtgttccta ttccgcaagc attgcagcca aaaaaagtta ttaatgtgaa
     8881 aggaaaagtt acggatgccg gaaccggtaa gccaattggc gctaatataa tcataattcg
     8941 agacctggat acactgaaca ccggagcatt acaaagcaat ccctatgatg gtaattttac
     9001 ctatgttttg acagaaggct atgcatatga agttatcatt actgctcctg gtttcaatgc
     9061 ccaaactcta aattatgatc ttaaggaatt gcaagactac aaactcataa atgaagtcgt
     9121 aaatcttact ccgtacaggg gcttgcttag attaacaata gttaattctc ttaacaaaga
     9181 tacaattacc accgctgtcg taactacatt atcaaatccg gttgaaggaa ttaatgggta
     9241 ttatgaaata ccggtatcgt ttggtattga gtataaaatt gagatgaatg ctaagggatt
     9301 tgaacctggg caaatggatt ttcaggtttc agacataatg ccaaaaaagg tctataagaa
     9361 aagcatagaa ctgcagcctg ctttgcctga acttattata gtggtgaacg acaaggaatc
     9421 tggagaaaaa ttaaaagtgt atctggagat ctttgacaaa accaaaagga ctctgaaata
     9481 taaaggaatg atggaggttg gtgactattc gggaaatctt gaacacggcc atgattatat
     9541 aatgaggata acccggccag ggtatttctt ctttgaagaa ggaattaatc tgagtgaaat
     9601 tttttacggt ttgaaaatcg agaaatctat tgtgatgact ccgctcaaaa aaggagaaaa
     9661 gctgactttg aataatataa tctttgaatt gaacagtgct gacctgacgc aggactccaa
     9721 aacagagctg gataacgtat ttgagctttt aaaacagaac agaaatattg tgctggagat
     9781 tgccgcacat actgatgacc ttggtgatga tacttataac cttcaacttt ccaatgcaag
     9841 agctaaatca gtcaaggaat atttattatc attgggtgtg cccgaaatga tgcttgtttc
     9901 aaaagggtat ggagaatcga tgcccgcagt ccggaatgat tcagaagaaa accgaaaatt
     9961 aaacagacgg gttgaatttc tcgttcttga cgcgaaatga agtttattag gttaaatact
    10021 caaagaagct tgctgatttc attggatgca agagccaacg gagcaagtta tattggatat
    10081 aagggagaac ctttgttttg caaaaacact tccgggccag catcgtaaga aaggagtaga
    10141 ttaagtttta caagtttgat gagccaatta cgcaaaagtg atgacttttt ttgatcaaat
    10201 gaaatactcc cgatgcagta tggagagtat taataaagca atcagactgg aaagggaata
    10261 ataaattctg atccaaaaaa aattgatctt ttcactaatc aaattgctga aaatcatcta
    10321 tgttgaagtt ttagttgtct tccaaaattt gtaatttgtg aagacaataa atatgcatca
    10381 tgtctccaaa cttcaaattt aaccttcttg ctattaccat cttccttttt gttttacacc
    10441 ctgtatcatc tcaggagtta gtcagagtta ctcctgaaga agttggaatg tcttcggagc
    10501 gactgggaag agttaccggc gtttttaacg aatatgttga agcaggtaaa atagctggaa
    10561 gcgtaatcct tgtgatgcgt aagggaaaag tagcttactt tgaagctttt ggttatagaa
    10621 acgtggagga caaattaaaa atggaaagaa ataccatttt ccgtattgca tcgcaaacaa
    10681 aagccattac aag
//
LOCUS       DASWHX010000215        15946 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_2112280, whole genome shotgun sequence.
ACCESSION   DASWHX010000215 DASWHX010000000
VERSION     DASWHX010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 15946)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15946)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15946
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2112280"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1378
                     /locus_tag="VGA21_14100"
     CDS             <1..1378
                     /locus_tag="VGA21_14100"
                     /inference="COORDINATES: protein motif:HMM:NF025486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="putative porin"
                     /protein_id="HEX9651691.1"
                     /translation="KLTVDKQIGNVIEGDRSVKGTSYDAFGSFHNKKKTYWIMANYSR
                     TRHVAFETGGVDEGSIGAFRDYFDDDAEPVMIGPESIEKRRNIHSYQELQLLKPVKMY
                     HEFDRYYQKNSFSFVNDDSGPAEFFDNFYVDTVATVDESHFSYYKNEGGLKGRSGKLF
                     YNAFYKNRLIDFDYKYIDSLALDFKTKRPEHYIGGQVIYNLDGVTDAKVSMEYLLEGN
                     YKIKASISTKWGEIGYFNSRYQPSFIQRSYFGNHDRWSNDFKDPIFLQVNARGKIMAG
                     PIELRPEVAFTQISHYIFFSSDTIPAQASNTVGYLSPGIDFSVKLPLNFTFQSEIIYT
                     HFLNETRSFVQIPKFLVNGHLYYKNMLFKGALDTQIGAKIYWREDYYADNYYPILQQF
                     FYQDEFKMPTSLKIDLYLSFKVRSALVFFKFVNLKQMLIGEGNLVAPFYPQQRNAFDF
                     GFTWFFYD"
     gene            1384..1974
                     /locus_tag="VGA21_14105"
     CDS             1384..1974
                     /locus_tag="VGA21_14105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010610060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-(ms[2]io[6]A)-hydroxylase"
                     /protein_id="HEX9651692.1"
                     /translation="MAFKLGLKLPTDPRWVNIAEKNLEEILTDHAYCEQKAASSCISM
                     IIQFPELDELVETLTPVVAEEWEHFERVLAHLRKRGFSLGKPRKDLYVQKLNEVIRKG
                     GSREDQLLEKLLQNALIEARSCERFRLLWKGINEGDLSEFYYELMVAEAGHYKNFLLL
                     AKRYVAPEKVEKRWKQLLKEEAEIMKSLEARGDRMH"
     gene            complement(1971..3233)
                     /locus_tag="VGA21_14110"
     CDS             complement(1971..3233)
                     /locus_tag="VGA21_14110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009578116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthetase B"
                     /protein_id="HEX9651693.1"
                     /translation="MKKALVIILFSTLLISGSSASYIFIPMDETQANHLKAYGIAYWV
                     LTNEIEVDWLLNYRGGSFMMKYHQKFENELVIRGVSYEVISDAQSNNIIASIASPSSN
                     MDIMKLEKFPKIAVYSPKSKQPWDDAVTLVLTYAEIPYDMIFDDEAMYGELSRYDWLH
                     LHHEDFTGQYGKFFRSFRNFAWYINQQREFEQSARKHGFEKVSELKREIVKKIRDFVS
                     GGGFLFAMCSATDTFDIALASDGVDIVDWMYDGDPPDPKAQQKLNFGSTFAFTDFRLS
                     RDPYEYEFSNVDNQSRERGLNQNNDYFQLFEFSAKWDPIPTMLTQDHTRTIKGFMGQT
                     TGYKKDLIKPDVVVMGENKSISEAKYIHGVFGKGFWTFYGGHDPEDYQHIVGEEATDL
                     NLHPNSPGYRLILNNILFPAAKKKKQKT"
     gene            3331..4569
                     /locus_tag="VGA21_14115"
     CDS             3331..4569
                     /locus_tag="VGA21_14115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009185853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9651694.1"
                     /translation="MDLRENIREGLKSIQANLLRSILTALIVAIGITSLVGILTAIDG
                     IQSSINDNFSTLGANSFTIRAKQGSRQTQRGITEKSYPDLTFKESNQFVDNYAYPAEI
                     SIYTYVTGIAEIKRLSEKTNPNVRVLGANEYYLSIKGLEISKGRNFSSIETRYGSEVV
                     ILGQAIRDALFKENEDPINKMIGILGSKYKIIGVIEEQGSLGGGGGIDRSVVVPVQAA
                     TRLGADRRLSYTVEAAISDPTQMEAAMGEATGVMRKVRRDRIGSEESFEIVRSQSLAE
                     RLEEITGYLRIGGFGIGFITLLGASIGLMNIMMVSVTERTREIGIRKALGATPAKIRQ
                     QFLIEAIVVCQLGGILGIILGIGIGNLVSSLIASGSFLIPWFWIIVAFLIGVIVGLSS
                     GYYPAFKASRLDPIDALRFE"
     gene            complement(4512..5876)
                     /gene="mtaB"
                     /locus_tag="VGA21_14120"
     CDS             complement(4512..5876)
                     /gene="mtaB"
                     /locus_tag="VGA21_14120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009580991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HEX9651695.1"
                     /translation="MKKVAFYTLGCKLNFSETSTLSRMVESKGFQKVEFDDKPDIFII
                     NTCSVTENADKKCRKIVRDALRINKDGYIAIIGCYAQLKPKEIAEIPGVNAVLGAAEK
                     FQLPDLLETFHKRNEKVIMASEIEQIHDFHHSFSIHDRTRTFLKIQDGCDYSCSFCTI
                     PLARGESRSDTIRNILLHVKQIAANKVKEIVLTGVNTGDFGIQNGKRSESFIDLAKTL
                     DQVEGIERFRISSIEPNLLTDEIISFVSQSVKFVPHFHIPLQSGSDEILGLMRRRYQT
                     SLYTSRINQIRKLMPNACVGVDVIAGFPGETNAHFLETYDYLNHLDVNYLHVFTYSER
                     ADTKAAVMENSVPVKIRNERSKMLRILSEKKRRLFYESQLGAELEVLFEHDIEDGRIH
                     GFTPNYVRVSHLYNESLINSIQKVRLINISNNGTVDCEILLTADYSNRRASIGSSLEA
                     LNAG"
     gene            6103..8265
                     /locus_tag="VGA21_14125"
     CDS             6103..8265
                     /locus_tag="VGA21_14125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009577803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HEX9651696.1"
                     /translation="MALIRTRALEMVANRKTERIVPPADKISDYFGVNSFNLNTMKSM
                     LSQHVFRRIAKAIAEGTPIDAEAAEALASSAKNWAISKGTTHYTHWFQPLTGATAEKH
                     DSFFNAISGIEEFKGSTLVQQEPDASSFPHGGMRSTFEARGYTAWDPSSPLFIWGRTL
                     CIPSVFVSYTGEALDYKAPLLKAVEALDKIATRICQLFDKDVAKVTPTLGCEQEYFVI
                     DKALFSARPDLVMAGRTLFGHNPARGQQLDDHYFGSIPSRIYNFMKDFEIEALQLGIP
                     VSTRHNEVAPSQFEVAPIFEDINVATDHNQLLMDVMNRVADRHDLKVIFHEKPFAGLN
                     GNGKHNNWSLMTNTGVNLLAPSSSARENLQFLVFLVNTIKAVHDYADLLRASIASAGN
                     DHRLGANEAPPAIISVFIGAELSAVLDELESSGNVRIRKGDNMYMKMGIDKIPQIIVD
                     NTDRNRTSPFAFTGNKFEFRAVGGNENVAAPMTVLNLIVAEQLRIFLDEANKEMQKGT
                     EKRLAIANILKKYIKDSKKIRFEGDNYSQVWQKEAARRGLSNIKDTPRALDEYLNPKS
                     VKLYEKHGVLVKRELEARNEIRLENYILKIQIEARVMGDLILNHIIPTAINYQNKLLT
                     NAKGLKELGVDNKEVVRNIKAIGVHIKTISTLVEKMVQDRRKINKIDDQREKAIQYCD
                     NIKGKYFDTLREAVDALEFLVDDEDWPLVKYRELLFLR"
     gene            complement(8315..8689)
                     /locus_tag="VGA21_14130"
     CDS             complement(8315..8689)
                     /locus_tag="VGA21_14130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014798685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651697.1"
                     /translation="MDKRRIVKDYDALPEEVVSQLKLEYPHGFAQNLISFTNKEGKKV
                     SALPFETDEVYYLVRMTTLEAERIIEDDEDYDDEGNLREDFSAEIEEEIVTDSAEEEE
                     SSGFDHDDPIRDQAGEEEEEED"
     gene            complement(8793..9674)
                     /locus_tag="VGA21_14135"
     CDS             complement(8793..9674)
                     /locus_tag="VGA21_14135"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9651698.1"
                     /translation="MFSYRKKWLRKIVILTAVVVITPHLISGCFSFRMSPKEIAEYYR
                     DQYHQPVIKKIKFEGRNINFATIGSDSLPMVIFVHGAPGSWNAFRHFLKDADLYSKAQ
                     LVAVDRPGYGYSDLGMPETRVAEQSKLLSALFQYNKSGKPAILVGHSLGGPIAARMSI
                     NYPKEVGALILVSPSIDPGLEPEEWYRFPLALPFIRWILPASLDMTNREILHLRKELE
                     QMLPLWEEIKAPTTVIQGSKDNLVDPDNAKFAQKMIINAPLKIQVETEADHFIPWNNP
                     GLIKSAILSYLEEVENP"
     gene            complement(9676..11337)
                     /locus_tag="VGA21_14140"
     CDS             complement(9676..11337)
                     /locus_tag="VGA21_14140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009577809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEX9651699.1"
                     /translation="MKRNKRIIGLGLLLVFTTAFVAFRSSDRYFEIARNLEIFAAVYK
                     EINNYYVDEVNPNTLMRTGIDAMLQSLDPYTNYIPEDDIEDFRYETTGQYGGTGIQIT
                     KMNGKTMITKVFEGYSAFRAGIIVSDEITKVNGVEISALNLDETGKLMRGQLGNHITL
                     SVLKYGESQARDFSLKIEKITDPNVPYYTMLTDDIGYFLLVGFTFGAGDEAQQAVRNL
                     INKGAKKIILDLRENPGGIVDEAVAITNIFISKGIEVVYTRGKVKDASVNRSFQTRSE
                     PVNLSIPLVILINSGSASASEIVAGTIQDLDRGVIIGEKSYGKGLVQQPRSIPYNSQV
                     KITIAKYYIPSGRSIQSLDYAHRNPDGSVGTVPDSLKTAFLTKNGRTVYDGGGIDPDV
                     EVHSLELSSIGQAMDENGYFFEFAARYKSRHSRIEPPRNFRLADAEFEEFVNWVSEKE
                     YEYTTDLDRELEDLIQSSKYEKYFLSIENQLIDLQETIEINKKSDIHLHKDELKMLLE
                     SYIVSMYYPESGIIEANFKHDEVLGKAIDVLENSSEYKSLLSSGK"
     gene            complement(11410..12408)
                     /locus_tag="VGA21_14145"
     CDS             complement(11410..12408)
                     /locus_tag="VGA21_14145"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017731466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HEX9651700.1"
                     /translation="MRRSKIIGVGHYVPERVVTNDDLAKIMDTSDEWITERTGIKERR
                     YFDPEKDTVSNMSATAAKMAAERAGISMKEIEFIVLATITPDYFFPGSGVLLQRELGL
                     HGIGALDLRNACSGFIYGLSVADQFIRTGMYNTILVIGAEIQSSALIMDSEHRGTNVI
                     FADGAGAAILRATDNENQGILSTHLHADGRYAEELFVKDPGSSRKVRLSHELIDGDTF
                     YVHMNGRDVFKHAIVRFHEVIQEALAANNLKQTDIDLLIPHQANLRISQHVQKQLGLS
                     DDKVFNNIMRYGNTTAASIPIAMSEAWSDKKIQAGNLICLASFGSGFTWASALIRW"
     gene            complement(12456..13154)
                     /locus_tag="VGA21_14150"
     CDS             complement(12456..13154)
                     /locus_tag="VGA21_14150"
                     /inference="COORDINATES: protein motif:HMM:NF014626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HEX9651701.1"
                     /translation="MIERYSISRPALNLEERFGIEVDKKYKPKYNAAPTQLVPIITLS
                     GRGGLSYFHWGQTPELAKAKPVGQKLLFADSSDLTIKANLKRKLAAHRCIIPADGFYA
                     WKKVGKKEMIPYRIVTLNQVLFSIAGLWEEYEDESGNEVHTFSMIQTKSPQVIEQITP
                     SLPAILHQDEEEIWLEKSSGSDDLISIIKPYSNDELSFFTVSSRIHSLNAEGPELAKP
                     APAADQFGNYSLFD"
     gene            complement(13177..14142)
                     /locus_tag="VGA21_14155"
     CDS             complement(13177..14142)
                     /locus_tag="VGA21_14155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019597251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX9651702.1"
                     /translation="MKFQKNNIEQHFSPPIIQFNGHLQTIIPGLFRRIKINGLQRERW
                     VTPDGDFIDVDWLKNSDKRLVILCHGLEGDSRRPYILGMIKALASRNFSTLSWNFRSC
                     SGEINKLKRFYHSGATEDLHFVIHKAIEKLAPEEIFIIGFSLGGNLALKYLGENHNLS
                     PLIKKTVGISVPIHLASSCKKMMNYENRLYEIRFLRSLKGKVISKEKLMPGTFDLKPL
                     TRIHHLWDFDDLYTGPIHGFKDAPDYYEMCSAINFLKDINIPTLLINAKNDPFLSEKC
                     YPDNQQISNPLVTTIYPDKGGHCGFSASNGLYYSEYVTLEFLSKD"
     gene            14343..>15946
                     /locus_tag="VGA21_14160"
     CDS             14343..>15946
                     /locus_tag="VGA21_14160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009583509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEX9651703.1"
                     /translation="MKYLSLTSLLFLFFQLNALFGQNKFTLNGYIRDAESGEELIGAT
                     VFVTNSGSGGVTNAYGFYSVTLPAGTYVIRYAYLGYKTTETKVELSKDVTQNVDLEEE
                     SVEIEEMVITAERAQQNFEEINMSRNELDVDQIKKLPALFGEPDIIKTIQMLPGVITA
                     GEGTSSYFVRGGSADQNLILIDEAPIYDPSHLFGLFSVFNGDVIKDADLYKGGIPARF
                     GGRLSSILDVRTRDGNNKKIHGSAGIGTLASKLLVEGPLRKEKSSFLVSARRSYADIF
                     IAAAGEDARVSFYDINAKANWRLNNKNRFFAAFYVGKDSFSFNKIFGFDWGNRTATLR
                     WNHHYTEKLFSNLTLIGSQFAYGLELKDPVQGFEWTSRIQQFTLKADNHYFASPKVEI
                     KFGYDLSYRRFFPGKIEPNQPGSIFTTSELEHLFALDHALYLESKQTISERFTLELGV
                     RLSIFQNIGETTIKYYEDINNRSSTIVTGERKYDQFENIKTFVNPEPRVSGRFLLKSQ
                     SSLKASYNRMVQNVHLVSSGTVPLPL"
BASE COUNT         4785 a         3086 c         3219 g         4856 t
ORIGIN      
        1 aaaattaacc gttgataagc aaattggcaa cgtaatagaa ggcgacagaa gcgtcaaagg
       61 aacctcatac gatgcatttg gcagttttca taataagaaa aaaacgtact ggataatggc
      121 taattattcc cgaaccaggc atgttgcatt tgaaaccggt ggagtagacg aaggtagtat
      181 cggagcattc cgggattatt ttgatgacga tgctgagcca gttatgattg gcccggagag
      241 tattgaaaag aggaggaata ttcacagtta tcaggaattg caattgctta agccggttaa
      301 gatgtatcat gaatttgaca ggtattacca gaagaattcc tttagctttg ttaatgatga
      361 ctcaggtccg gctgaatttt ttgacaactt ttatgtcgat actgttgcta ccgtggatga
      421 gtcgcacttt agctattaca aaaatgaagg tggcttaaaa ggtagatctg gaaaattatt
      481 ttataatgcc ttttacaaaa atagattaat tgattttgac tataaatata ttgactccct
      541 ggctcttgat ttcaaaacaa agaggccgga gcattacatc ggcggtcagg ttatctataa
      601 tttagacggt gttacagatg caaaggtatc tatggaatac ttattggaag gaaactataa
      661 aattaaagcg agtattagta ctaaatgggg tgaaattgga tattttaatt caaggtacca
      721 accttcattc atacaaagaa gttattttgg taaccatgac agatggtcaa atgattttaa
      781 ggatccaatt tttcttcagg ttaacgccag aggaaaaatt atggccggtc ctattgaact
      841 aagacccgaa gttgctttta cgcaaatcag tcactatata ttttttagtt cagataccat
      901 acccgcgcaa gcttcaaata ctgttggtta tctaagtccg gggatagatt ttagcgtaaa
      961 attacccctg aattttactt ttcaatctga aataatttat acccatttcc ttaatgaaac
     1021 ccggtcattt gtgcagatac ccaaattttt ggttaacggt cacttgtatt acaaaaacat
     1081 gctgtttaag ggtgctttgg atactcaaat tggagcgaag atttattggc gagaagatta
     1141 ttatgcagat aactattacc caatactgca gcaattcttc tatcaggatg aattcaaaat
     1201 gcctacctcc ctgaaaattg atttatacct gagttttaaa gtcagaagcg cattggtctt
     1261 cttcaaattt gtgaatttga aacaaatgtt gattggtgaa ggcaatcttg tcgcgccatt
     1321 ctatcctcag caaagaaacg cctttgattt tggctttacc tggttttttt atgactaatt
     1381 caaatggcat ttaagctcgg cttaaaactc cctactgatc cacggtgggt aaacattgca
     1441 gaaaagaacc tggaagaaat actcacagac catgcctatt gcgaacaaaa ggcagcttct
     1501 tcatgcattt ctatgatcat acaatttccg gaattagatg agttagtaga aacactaaca
     1561 ccggttgtcg ctgaagaatg ggaacatttt gaaagggttc tggcgcattt gaggaaaaga
     1621 ggattttccc tgggaaagcc tcgcaaggat ttgtacgttc agaaattaaa tgaagttatt
     1681 agaaaaggag gaagccggga ggatcagcta ttggaaaaat tattgcagaa cgcgctaatt
     1741 gaagcaaggt cctgtgaacg attcaggttg ctatggaaag gcataaatga aggcgaccta
     1801 agtgaattct attacgaact tatggttgcc gaggctgggc actataagaa cttcctatta
     1861 ttggctaaaa ggtatgttgc tccggaaaaa gttgaaaaac gctggaagca attgttaaaa
     1921 gaagaagctg aaataatgaa atcccttgaa gcccggggtg atcgaatgca ttaagtcttc
     1981 tgctttttct tttttgcagc ggggaataaa atattgttta aaataaggcg atatcccggt
     2041 gaattgggat gcaaatttaa atctgttgct tcctcgccaa caatatgctg gtagtcttca
     2101 ggatcatgcc cgccataaaa tgtccaaaag ccttttccaa acaccccatg aatatatttt
     2161 gcttcactta tagatttatt ctcacccatt acaactacat caggttttat caaatctttt
     2221 ttgtagcccg ttgtttgacc cataaatccc tttatggtac gtgtatgatc ctgagttagc
     2281 atagtaggaa taggatccca ttttgcactg aattcaaata attgaaagta gtcattgttc
     2341 tgattgagtc cgcgctcccg tgattgatta tcgacatttg aaaattcata ttcataagga
     2401 tctctggata atctgaaatc ggtaaaagca aaagtgcttc caaagttcag tttttgttgt
     2461 gctttcggat caggagggtc gccatcatac atccaatcca cgatatcgac accatctgaa
     2521 gcgagcgcaa tgtcaaaagt atcagttgcg gaacacatgg caaataagaa tcctccaccg
     2581 gaaacaaagt cgcggatctt ttttacgatt tccctcttca gttctgaaac tttctcaaaa
     2641 ccatgcttcc gcgcggattg ttcaaattct ctctgctggt taatatacca ggcaaagttt
     2701 cgaaagcttc tgaaaaattt accgtactga cctgtgaaat cctcatggtg taaatgcagc
     2761 cagtcataac gtgataattc tccatacatg gcttcatcat cgaagatcat atcatagggt
     2821 atttccgcgt atgtaagaac aagggttact gcgtcatccc agggttgttt actttttgga
     2881 gaataaacgg caatttttgg gaacttctcc agcttcataa tatccatatt agaacttgga
     2941 ctggctatgg aggcaataat attattggat tgggcatcac taattacctc ataacttacg
     3001 cccctgataa ccagttcgtt ttcgaacttc tgatgatatt tcatcataaa gcttcccccc
     3061 ctgtagttaa gtaaccaatc cacttcaatt tcgttagtta atacccagta agcaatgcca
     3121 tatgctttaa gatggttggc ttgagtttca tccataggaa tgaaaatata actggcagaa
     3181 cttccggata ttagtagagt ggagaataaa atgataacta atgctttctt cataattcaa
     3241 ccgttaaatc agccatctat cttaattata acgaaattta tgtaattata gatattgaaa
     3301 gcacttaaaa gcaataattt agacctataa atggatttaa gggaaaatat aagggaaggg
     3361 ttaaagtcaa ttcaggccaa tttgcttcga tcaattctaa ccgctttgat agttgccatt
     3421 gggattacct ccttagttgg aatacttact gcaatagatg gaattcagtc cagcattaac
     3481 gacaatttct ctactttggg agcgaattcc tttacgatca gggctaagca gggaagccgg
     3541 cagacgcagc gtggtattac tgaaaaatcc tacccggatt taacttttaa agaatctaat
     3601 caattcgtcg ataattatgc ttacccggcc gaaataagta tttataccta tgttaccggt
     3661 atagctgaaa ttaaacgact gtcagaaaag accaacccga atgtcagggt tttaggtgcc
     3721 aatgaatatt atctgtccat taaagggctt gaaatttcaa agggcagaaa tttttcaagc
     3781 attgaaacgc gatatggttc cgaagtagtg atccttgggc aggccataag ggatgcattg
     3841 tttaaagaaa atgaagatcc gataaataaa atgattggca tcctgggttc aaaatataaa
     3901 ataattggag ttattgagga gcagggttcc cttggtggag gtggtggtat agacaggtca
     3961 gtagtagtac cagttcaggc ggccacaaga ttaggagcgg atcgaagact atcctacacc
     4021 gttgaagccg ctatttccga tccaactcaa atggaggcag cgatgggaga ggccactggg
     4081 gttatgagaa aggttagaag agatagaata ggaagtgaag aatcttttga aatagtaaga
     4141 agtcagtccc tggcagaacg attggaagaa ataacaggtt atttgcgtat tggtggcttt
     4201 ggtattggat tcataactct gcttggagct tccataggtc taatgaatat tatgatggtt
     4261 tctgtaaccg aaaggacccg tgagattggg attcgaaaag ccctgggcgc taccccggcg
     4321 aaaatcaggc agcaattcct gatagaagca atcgtggtat gtcagttagg aggtattttg
     4381 ggaattattc tgggaattgg aattggaaac ctggtcagca gcttaatcgc atccggatca
     4441 tttcttattc cctggttttg gattattgtg gcattcctga ttggagtgat cgtgggcctt
     4501 tcctccggat attatccggc attcaaagct tcaagacttg atccaataga tgccctgcga
     4561 tttgaatagt cagcggttag tagaatttca caatcaacag ttccattatt gctgatattg
     4621 attaacctca ctttctgaat actattaatc aggctttcat tatacagatg tgagaccctc
     4681 acatagttcg gtgtaaaacc atgaattcgg ccatcctcaa tatcatgctc aaacaatact
     4741 tccaactccg ctccaagctg gctttcataa aataagcgtc tctttttctc agacaagatc
     4801 cttaacattt tcgatcgctc attcctgatc tttaccggca cagaattttc cattaccgcg
     4861 gcttttgtat ccgccctttc tgaataagta aatacatgga ggtagttaac atctaaatgg
     4921 ttaagataat cataagtctc aagaaagtgt gcgttggttt cacctggaaa gcccgcgatt
     4981 acatccactc caacacaggc atttggcatt agtttcctta tttgatttat ccggctggta
     5041 tatagtgagg tttgataacg tcttctcata aggcctaata tttcatctga tccggattga
     5101 agagggatgt gaaaatgtgg aacaaatttc acagattgac ttacaaagct tatgatttca
     5161 tcggttaata aattaggttc tatcgagctg attctgaaac gttcaattcc ttcaacctga
     5221 tctaaagttt tggcaaggtc aataaatgat tctgatcttt ttccattttg aattccgaag
     5281 tcaccggtat ttacgcccgt caatactatt tcctttacct tattggcggc tatctgtttt
     5341 acatgaagaa gaatattccg tatggtatca cttcttgatt ctcccctggc aagaggaata
     5401 gtacaaaatg agcaggaata atcgcatcca tcctgtattt tcagaaaagt ccgggttctg
     5461 tcatggatgg aaaaggaatg gtgaaaatca tgaatttgtt caatttcaga agccatgata
     5521 accttttcat tccgcttatg aaatgtttcc agaaggtcag gtaattgaaa tttctctgct
     5581 gctcccaaaa cggcatttac tccaggtatt tctgcaattt cctttggctt tagttgtgca
     5641 tagcagccaa taattgcaat gtaaccatcc ttattaatac gtagagcatc cctaacaatc
     5701 ttcctgcatt ttttatctgc attttccgta acgctgcagg tattaataat gaagatgtct
     5761 ggtttgtcat caaactcaac tttctgaaat cctttggatt caaccattct ggaaagggtt
     5821 gaagtttcag aaaaattgag tttacagccc aaagtataaa aggcaacttt cttcataatt
     5881 gcaaagttat cgtatttaaa aaaggttgac aacagggtaa attccggccg ctttttccgc
     5941 tgaaaccggt tttgtagtta gcagcagggt caaattctac cttaatcttc aaaaaaaata
     6001 ataaaaaata gaagatacgg gtataatatg accttaatat ggaaataaaa tcattttatt
     6061 ctctaacttc gcctcaaatt aacctaccaa acttaaatta acatggcatt aatacgaacc
     6121 agagcgctgg aaatggttgc caatcgcaaa acagaacgga ttgtacctcc agccgacaag
     6181 atttccgatt atttcggggt taattccttc aacctgaata cgatgaaatc aatgttgtca
     6241 caacatgttt tcagaagaat cgcgaaagct atagcggagg gcacacctat tgatgccgaa
     6301 gcagcagagg ctttagcttc ttcagcaaaa aactgggcaa tttccaaagg aacaacgcat
     6361 tatactcatt ggtttcagcc acttaccggg gcaacagcgg aaaaacacga ttcctttttt
     6421 aacgcgatct ctggtattga agaattcaaa gggagtaccc tggtgcagca agaacctgat
     6481 gcatcatcat tcccacatgg agggatgaga agtacttttg aggcaagagg gtacacggcc
     6541 tgggacccaa gctctccgct ttttatttgg ggaagaactc tttgtatacc ttcagtcttc
     6601 gtctcttata caggtgaggc gttggattac aaagcgccgc ttttgaaagc tgttgaagcc
     6661 ctcgacaaaa tcgccacccg gatttgtcaa ttgttcgata aagatgtagc caaagtaact
     6721 cctactcttg gttgtgaaca ggaatacttt gtcatagata aggctttatt ttctgcccgg
     6781 ccagacctgg taatggcagg aaggacgcta ttcggtcaca acccggccag gggtcagcaa
     6841 ttagatgacc attattttgg atcgattccc agcaggatat acaattttat gaaggatttt
     6901 gagattgagg ccctccagtt gggaattccg gttagcacaa gacataatga ggttgccccg
     6961 agccagtttg aagtggctcc aatttttgaa gacataaatg tggctacaga tcacaatcaa
     7021 ctcctgatgg acgttatgaa tagagttgca gaccgtcatg atctgaaggt aatatttcat
     7081 gaaaaaccct ttgccggatt aaacggaaac ggcaaacata ataactggtc tcttatgacc
     7141 aatacgggcg ttaacttgct ggcaccttcg agttcagcaa gagagaatct ccaattcctg
     7201 gttttccttg tgaacacaat aaaagctgtt catgattatg ctgacttact aagagcgagc
     7261 attgcttcag ccggcaacga tcacagactg ggagctaatg aagcgcctcc ggcaatcatt
     7321 tctgttttta taggcgcaga actatctgct gttctggatg agctcgagag cagcggaaat
     7381 gtacgcataa ggaaaggaga caatatgtat atgaaaatgg gtatcgataa gataccccag
     7441 atcattgtag ataatacaga tagaaacaga acatcgcctt ttgcctttac aggtaataaa
     7501 tttgaattca gggccgtggg tggtaatgag aatgttgccg ctcccatgac cgttttaaat
     7561 ctgatcgttg cagaacaatt gcggattttt ctggatgaag ccaacaagga aatgcagaaa
     7621 ggaaccgaaa aaaggctggc aattgccaat atccttaaaa agtatattaa agattcaaaa
     7681 aagattcgat ttgagggaga taattactcg caggtttggc aaaaagaagc ggcaaggcgg
     7741 ggcttatcca atataaaaga tacacctagg gcgctggatg aatatttaaa cccaaaatca
     7801 gttaaacttt atgaaaaaca cggagtcctg gttaagagag aattggaggc tcgaaatgaa
     7861 attcgattgg aaaattatat tttgaaaatt cagattgaag cccgtgtaat gggtgacctg
     7921 attctcaacc atataatacc tacggcgata aattatcaaa ataaactatt aacaaatgca
     7981 aaaggtctta aggagttggg tgttgataat aaagaagtag tcaggaatat aaaagccata
     8041 ggagttcata tcaaaacaat ttctaccctg gtggaaaaaa tggtgcagga cagaaggaag
     8101 attaataaga ttgatgatca aagagaaaaa gcaattcagt actgtgacaa tataaagggt
     8161 aaatattttg ataccctcag ggaagctgtt gatgctttgg aatttttggt tgatgatgaa
     8221 gactggcctt tggttaaata ccgggaattg ttgtttttaa gataatgatt gaatacgaag
     8281 gtgtgggaat gccttgcaga aattctggcg tttcttaatc ttcttcttct tcttcttccc
     8341 ctgcctgatc tcggatgggg tcatcatggt caaagccact ggattcttct tcctcagcgg
     8401 aatccgttac tatttcttct tcaatttccg cgctaaaatc ttcccgcaga tttccttcat
     8461 cgtcatagtc ttcatcgtct tcaatgattc gctctgcttc cagagtcgtc atcctgacca
     8521 ggtagtaaac ttcatcagtt tcaaacggca aggcagaaac ttttttccct tctttattgg
     8581 taaatgaaat taagttttgt gcaaacccgt gtggatactc caatttcagt tgtgatacaa
     8641 cttcttcagg cagcgcatca taatctttaa cgattcttct cttgtccata ctgttactcc
     8701 ataacggcgg gcctaaaata ggagaggccg cccaagtaat caataccaat agagcataaa
     8761 agtaaaaaat ctgcgaaaag tatcaagagt aattatggat tttcaacctc ttccaaataa
     8821 ctaagaattg cagacttgat tagtccggga ttattccatg gaataaaatg atctgcctct
     8881 gtctcaacct gtatttttag cggagcattg ataatcatct tttgggcgaa ttttgcatta
     8941 tccggatcta ccaaattatc cttgctgccc tgaatcacag ttgttggagc tttgatctct
     9001 tcccaaagtg gaagcatttg ttctaattct ttcctgagat gaagtatttc tcggttcgtc
     9061 atatccagag aggcaggtaa aatccacctt atgaatggta atgccaatgg aaacctgtac
     9121 cactcttcag gttccaaacc aggatcaatg gatggagaaa caagaattaa tgcaccaact
     9181 tcttttggat aattaatcga cattcgtgca gcgattggtc cgccaaggga atgtccaaca
     9241 agaatcgcgg gcttaccgga tttattatat tgaaacaagg ctgacaataa ttttgattgt
     9301 tctgcaactc tggtttccgg catgcctaag tctgagtatc catagccagg cctgtcgacg
     9361 gcaacaagct gagcttttga ataaaggtca gcgtccttca gaaaatgcct gaatgcattc
     9421 caggagccgg gagcgccatg tacaaagatt accataggca gggaatcaga tccaattgta
     9481 gcaaaattaa tattccggcc ttcgaactta atttttttga taacaggttg gtggtattgg
     9541 tctctgtaat attcggcaat ttcttttggt gacatcctga aagaaaagca ccccgaaata
     9601 agatgcggag tgattaccac cactgcagtt agaataacga tttttcttaa ccattttttc
     9661 cggtaggaaa acattttatt ttccagaact taataaggat ttgtattcac ttgagttttc
     9721 taacacatct atagccttac ccagaacttc atcatgcttg aagtttgctt caattatgcc
     9781 ggattcagga taatacatgg agacgatgta gctttccaat aacatcttta gctcatcctt
     9841 gtgaaggtgg atgtcggatt tcttgtttat ttctatggtc tcttgaaggt caatcaattg
     9901 attttctatg cttaaaaaat acttttcata cttggatgat tggattaaat cttccagttc
     9961 ccgatcaagg tcggttgtgt attcgtattc tttctccgat acccaattaa caaactcctc
    10021 aaactccgcg tctgctaatc tgaaattcct gggaggttct atcctgctat gccttgattt
    10081 atatctggcc gcaaattcaa agaaataccc gttttcatcc atcgcctgac cgatagagga
    10141 gagttccagt gaatgaactt ccacatctgg atcaatgccg ccgccatcat aaaccgtgcg
    10201 tccatttttt gttaggaaag ctgtttttaa actgtcgggt actgttccaa cacttccatc
    10261 tgggtttctg tgtgcataat caagagattg aatacttctg ccgcttggaa tatagtattt
    10321 tgcgatagta atttttacct gagagttata aggaatgctc cggggttgtt gaaccaatcc
    10381 ttttccgtag gatttttcgc caatgataac ccctcgatcc aaatcctgga tggtcccggc
    10441 aacaatttcg gaagcagaag cgctaccact attaattaaa attacaagag gaatgctcaa
    10501 attaaccggt tcgcttctgg tttgaaaact tctgttaacg ctggcgtcct tcaccttccc
    10561 ccgagtgtaa acaacttcaa ttcctttcga aataaaaata ttagtgatgg ctacagcttc
    10621 atcaacaatt ccaccgggat tttcccgtaa gtccaaaata attttcttcg ctcctttgtt
    10681 gataagattc cttactgctt gttgggcttc atccccggca ccaaaagtaa aaccaaccag
    10741 caagaaatag ccgatatcat cagttaacat ggtgtaataa ggaacattgg gatcggttat
    10801 cttttcgatt ttgagtgaaa agtccctggc ctgagattct ccatatttga gaacagacag
    10861 tgttatatga ttgccaagct gaccgcgcat cagtttgcct gtttcatcca gattaagggc
    10921 tgagatttca accccattta cttttgtaat ttcatcactt acgattattc ctgctctgaa
    10981 ggccgagtaa ccttcaaaaa cttttgtgat catggtcttg ccattcatct tggtgatttg
    11041 aatgccggtt ccaccatatt gcccggtggt ttcatagcgg aaatcttcaa tatcatcttc
    11101 aggaatataa tttgtgtaag gatcaaggga ctgaagcatc gcgtcaattc cagtgcgcat
    11161 taatgtattt ggatttactt cgtcgacata gtaattatta atttctttat atactgccgc
    11221 aaaaatctcg agattcctgg ctatttcgaa gtaacgatca gaacttctga aagctacaaa
    11281 agcagtggta aataccagca ggagaccaag ccctataatc ctcttatttc tcttcatatc
    11341 attccaggcg atttgtaaac taaatttacg aaaatggagt ctttttgttt ataaaaattc
    11401 aataataggt taccaacgga ttaatgccga tgcccatgta aacccacttc cgaatgaagc
    11461 aagacatatc aaatttcctg cctgaatctt tttgtcactc caggcctcgc tcattgcaat
    11521 aggaatactc gccgcggttg tatttccgta tcgcattatg ttgttgaaaa ctttatcatc
    11581 cgacaaaccg agctgctttt gaacatgctg gctaattctt aaattagcct ggtgcgggat
    11641 tagtaaatca atatccgttt gtttcagatt attggcagcg agcgcttcct gaattacttc
    11701 atgaaatctg acgatggcat gcttgaaaac atctcgtcca ttcatgtgca cataaaatgt
    11761 atcgccgtca atgagttcgt ggctcaacct gacctttcta ctgctgccgg gatcctttac
    11821 aaataattct tctgcatatc ttccatctgc atgaagatga gtagacagaa ttccttgatt
    11881 ttcattatcg gttgccctca ggattgctgc acctgcaccg tccgcaaata ttacatttgt
    11941 acccctgtgt tcggaatcca ttatcaaagc cgaggattga atttccgcgc caataaccag
    12001 tatcgtgtta tacatgccgg tgcgaatgaa ttgatcggca acactcaagc cataaataaa
    12061 tcctgaacag gcatttctta aatcaagtgc gccaattcca tgcaaaccca attccctttg
    12121 caaaagcaca ccagatcccg ggaagaagta atcgggggta attgtggcca ggacaataaa
    12181 ttcaatttct ttcattgaaa ttccggctct ctccgcggcc atttttgctg ctgttgccga
    12241 catattggaa accgtgtctt tttcagggtc aaagtaccgt ctttctttga ttccggttct
    12301 ttcggtaatc cattcatcgg aagtatccat aatttttgcc agatcatcat tggttaccac
    12361 tcgttcaggc acataatgtc caactcctat tatttttgat cttcgcataa cattaaataa
    12421 gtgtttctaa tatagcacct attattaaag ataggtcaat caaaaaggga ataattccca
    12481 aattgatctg ccgcaggtgc aggtttggct aattcgggtc cttctgcatt taatgaatga
    12541 attctggacg agactgtaaa aaatgataat tcgtcattgg agtaaggttt gattattgat
    12601 attagatcat ctgatcccga agacttttca agccagattt cttcttcatc ctgatgaagg
    12661 attgcgggaa gtgaaggcgt aatttgttca attacctggg gagatttagt ttgaatcatg
    12721 gaaaaagtgt gcacttcatt tccagattca tcttcgtatt cttcccataa tcctgcaatt
    12781 gagaatagaa cttgatttag ggtaacaata cgatagggaa tcatctcctt tttgcccacc
    12841 tttttccatg cgtaaaaacc gtcggcagga ataatgcatc tatgagcagc aagttttctt
    12901 ttgagatttg cttttatggt gaggtctgaa gaatctgcaa agagaagttt ttggcctaca
    12961 ggttttgctt tggctaattc gggagtttga ccccagtgaa aatagcttaa tccaccccgg
    13021 cctgataatg tgattatcgg caccaactgc gttggtgcgg cattatattt tggtttatat
    13081 ttcttatcca cttcgatccc aaatcgttcc tctaaattca aagcaggcct tgaaattgaa
    13141 tatcgctcaa tcataacagg ctaatttagc aaattcttaa tccttcgaga ggaattccaa
    13201 agtaacatat tctgaatagt aaagtccgtt tgaagcggag aacccacaat gaccgccttt
    13261 atccggataa attgtcgtaa ccagcgggtt tgaaatttgc tgattatccg gatagcactt
    13321 ttcagaaaga aaaggatcat ttttagcatt tatcaatagg gtaggaatat ttatatcctt
    13381 caggaaatta atagcggagc acatctcgta ataatccggc gcatccttaa atccgtgaat
    13441 cgggcctgta taaagatcgt caaagtccca aagatggtga atccgggtta agggttttaa
    13501 atcgaatgtc cccggcatta atttttcttt tgaaattact tttcccttta atgatctaag
    13561 aaaccggatc tcatacaggc gattctcata attcatcatt tttttacaac ttgaagccag
    13621 gtgaatgggt acagaaatac caacagtttt cttaatcaac gggctcagat tatgattttc
    13681 acccagatat tttaaagcca gattgccgcc aagactaaat cctatgataa atatttcctc
    13741 cggcgctaat ttctctattg ctttatgtat aacaaaatga aggtcttcgg ttgccccact
    13801 gtggtaaaat ctcttgagct tgtttatctc tccgctacaa cttctgaagt tccatgaaag
    13861 cgtgctgaag tttcttgagg ctaatgcctt tatcattcct aaaatgtagg gtctccggct
    13921 gtctccctcc aatccatggc acaatataac caatcgttta tctgaattct tcaaccagtc
    13981 aacatcgata aaatcaccat caggtgttac ccatcgttct ctttgcagcc cattaatttt
    14041 tattcttctg aatagacccg gtataatggt ttgcaagtgt ccattgaatt gaatgattgg
    14101 tggagaaaag tgttgttcta tattattttt ttgaaatttc attttggtat agtgggctga
    14161 aatttactta ttaaatattg aaattctgaa tttgagtggg ggtgggagtg atcagagttg
    14221 tctttaacta atacgaataa attcatcaaa acaattaaat gaaatatctc tttaataata
    14281 ggggtgtaat ttaataaagg atttactttt gccaccgtta atcacttgaa gcaatatatt
    14341 caatgaagta tttatcctta acctcactat tatttctttt ttttcaacta aatgccctgt
    14401 ttggtcagaa caagtttact ttaaacggat atatcagaga tgcggaaagc ggagaagaat
    14461 taattggagc aacagtcttt gttacgaatt caggttcagg cggagttaca aatgcttatg
    14521 gtttttattc ggttacgctt ccggcaggaa catatgtaat acgttatgca tatctgggtt
    14581 acaaaacgac agaaacaaaa gtggaattgt caaaagacgt tactcaaaac gttgaccttg
    14641 aggaggaaag cgttgagatt gaggaaatgg taataaccgc agaacgtgcc caacaaaatt
    14701 tcgaagagat aaatatgagc cgaaatgagc tggatgtgga tcaaattaag aagttaccgg
    14761 cattgtttgg agaacctgat ataatcaaaa caattcagat gttaccgggt gttattacag
    14821 ctggggaggg gacatccagt tattttgtgc gaggcggcag tgcagatcaa aatctaattt
    14881 taatagatga ggctcccatt tatgatcctt cacacctttt cggattattc tcggttttta
    14941 atggtgatgt tattaaagat gctgatttat ataaaggagg catacctgct cgtttcggag
    15001 gacgtttatc ttcaatcctg gatgtacgaa cccgtgatgg caataacaaa aaaattcacg
    15061 gctcggcggg cattgggaca ctcgccagta agctcctggt tgaagggcca ttaagaaaag
    15121 agaaaagttc gtttctggtt tccgcccgtc ggtcttatgc ggatatcttt atcgcggcag
    15181 cgggagaaga tgcaagggtt tcattctatg atataaatgc aaaggcaaat tggaggttga
    15241 acaacaaaaa cagattcttt gccgcatttt atgtgggtaa agattcattc agttttaata
    15301 aaatttttgg gttcgactgg ggcaaccgta ccgccacact tcgatggaat catcattata
    15361 ctgaaaaact tttttcaaac cttactctta taggcagtca gttcgcgtat gggttggaat
    15421 taaaagaccc tgtacaaggg tttgaatgga cttccagaat tcaacagttt actttgaaag
    15481 cagataatca ttacttcgca agtccaaaag ttgagatcaa atttggatat gatttatctt
    15541 accgcaggtt tttccctggt aaaattgaac ccaatcaacc cggctcaatt tttacaactt
    15601 cggaacttga acacctgttc gcactggatc atgccttgta ccttgaaagc aagcagacta
    15661 tatccgaaag atttacactt gagctgggag ttcgattgag catattccaa aacattggcg
    15721 aaactacaat aaagtattat gaagatataa acaacaggtc ttcaacaata gtgacaggag
    15781 aaaggaagta tgatcagttt gagaatataa aaacatttgt aaatcctgag cccagagttt
    15841 caggaagatt tcttcttaag tcccagagtt ctttaaaggc ttcgtataac agaatggttc
    15901 agaacgtgca cctggttagc tccggtactg ttccattgcc attaaa
//
LOCUS       DASWHX010000216        32356 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_1749901, whole genome shotgun sequence.
ACCESSION   DASWHX010000216 DASWHX010000000
VERSION     DASWHX010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 32356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32356
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1749901"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(263..1120)
                     /locus_tag="VGA21_14165"
     CDS             complement(263..1120)
                     /locus_tag="VGA21_14165"
                     /inference="COORDINATES: protein motif:HMM:NF042672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6624 domain-containing protein"
                     /protein_id="HEX9651704.1"
                     /translation="MEWRLAWRMWESESLDKNYALGAKQFDSLLAIKSNIDPNLLISG
                     IRCLAETNQYPKAYVVLLNNPRIFEINLCVEGWFRPPNTPEWGDICIRMDNNADLLFP
                     QIKEDLLIMYFNDQIARGREIRSLVNETGYSISKDKIYDEDIDGTDKINIEKLKIIID
                     EIGFPDRKMVGKQGMMSIYTIIRNANKYPEFQASQLKNVKLAAEQGELSTEDYAYLFD
                     RVKLNAGERQVYGTHFSAPDYADRKAVLMPITDEEKLDDRRRELGLMPIELFKRLLLF
                     EFEGTDSIS"
     gene            1387..1722
                     /locus_tag="VGA21_14170"
     CDS             1387..1722
                     /locus_tag="VGA21_14170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012925542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX9651705.1"
                     /translation="MKGTYLGEFEEVILLIVAILNGKAYGNSLKEEFQGRANRSVNLS
                     AIHAALYRLEQKGFVKSQFGEPTCERGGKRKRFFSATNKGLSALQESRDLRKNLWADV
                     PVNALSKLR"
     gene            1719..4379
                     /locus_tag="VGA21_14175"
     CDS             1719..4379
                     /locus_tag="VGA21_14175"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9651706.1"
                     /translation="MNKPPKIATWFLRLFCDPAFVEMIEGDLYEEFQTRLTSRGKKNA
                     TLYYWWSVIRSFRPYLLKRNSNPYKYTEIMLLHNYFKIAYRNLIKQKLYTSINVIGLT
                     AGMASSLLILLFVKSELTFDQHHERAEDVFRVSTNGESNGETMNMQTSPLGLAFKMKS
                     EFPEVEEIARLSSFVGVEKNLVSYEETKFFQDEGYLADSAFFKILKYDFAEGNPATCL
                     NNSGNIVISRSLAEKIAGNEPALGKIISIENSFGKVDYEITGVFKEPVKNTHLKISFV
                     TSMNNPGIGEYIFRSNELLGNNFVYSYLRLNQNASPQQLVSKFPAFLDKYVGEDMEKT
                     GFRKSHDLINVADIHLKAKGLGFQNEGDLKTIYILSTIAGIILLIACINFMNMATAQS
                     GHRSREIGVRKVFGAQRISLANQFLTESVLITLISLIVSIGIVYLLLPSFNQITGQRI
                     TLDLLEPAQLLIAVAIGFITGFIAGSYPAFHLSGISINRIFKNKSNKSGLGMVRKGLV
                     IFQFLISISLIVGVITINQQLNFVQSRSLGFQKENQIAIPLRSPEVGRQYKILENEFS
                     KLPGIVNIGAGHAYPGKFVFNDNSYYKDGSSMDEAVNIRTNYVDFKFMNTLGVEVVNG
                     REFSEDFPADVNTAVVLNEAAVRNLGYELDDVVGRNIHSMFPINGRDTIITYKVSGVV
                     KDFNFQSLYEDIIPYMFTMSNPDTFQPRYLLVSVNTNNYQNLFAEMKTTWDRQAGGIP
                     FEYTFMDDDLNSQYKSDESLSFIVKVFTTVAIIISALGLFALSSFTAESRLKEMGVRK
                     VMGASGKDIFLIMSGNFSILILISFVLSIPPGWYFIRTWLNSFAFRIDINPWIFLLAG
                     AVTFAFAILVVSYQCLKVSLVNPVNVLKDE"
     gene            4496..7861
                     /locus_tag="VGA21_14180"
     CDS             4496..7861
                     /locus_tag="VGA21_14180"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HEX9651707.1"
                     /translation="MRKLIIIISLIILATLLYRWFFRRPQSCNVLVFSKTAEFRHESI
                     EEGKLAIMKLGKENGFEVDTTEDAAIFKERNLRNYKVIIFLSTTGDVLNESQQLELTR
                     FIQAGGSFVGIHAAVDTEYDWPWYNQLVGAYFESHPQIQKATVKVVNPQHPATERLPT
                     EWERVDEWYNLKDIQPYINVLINLEESSYEGGKNGENHPISWFHEFDGGRVFYSGFGH
                     TSETFQEPEFLNHLLGGIQYAAGDGSPVNYDNVTVTPDARRFAKTVMESGLNEPMELE
                     VLKDGRVLYIERRGGIRMFFPQENKAKTIGRLEVFSEQEDGLLGLAKSPDFESTGWIY
                     LFYSPAIDDHVQYVSRFTIIDDTVDLSSEKIILKIPNQRDECCHSGGGLEFDSKGNLY
                     ITVGDNTNPFASDGYAPIDEREGRSAWDAQKSSANSMDLRGKILRIKPEADGTYSIPD
                     GNLFPKDDINSRPEIYIMGNRNPFRLSVDNKTGFIYWGEVGPDAAKDSTDRGPKGYDE
                     VNQARSAGFFGWPYFVADNKAYNDYDFTKNLSGRKFDARNPINNSPNNSGLGKLPSAQ
                     PAFIYYPYDASEEFPLTGTGGRNAMAGQVYYFEDYKNFANAFPEYYDGKLFIYDWMRG
                     WINVVTMDDPGNYTKMEGFLPEEKWIHPIDMVFSEKGELYIIEYGSIWYVGNADARLS
                     KISFISGNRAPVASITTDKIAGAVPLEVLVSARNSVDYDGDDLTYHWKLDGKLVPGIS
                     GDENLTISENGIHEVELTVTDSEGNVSKNSLDIYAGNTPPEIALEIAQNETFFRGGDL
                     KYSINLTDEEDSQNNKIDLSRLVIAGTFLPSGFELKNTLSFQDYLNNNPAYLGKILIE
                     NSDCQACHQERQSSVGPSYLEISNRYENNELNSKSLSGKIVNGSVGVWGTRPMIAHHQ
                     LNETETKLMVEYILNLDKPVKQSETQLSGIFELKEQSEKGIYVISASYTDQGANGIQP
                     LTTRDYMILRNPVLEAEDFDFSNQIMTYSAKAGEVPILDRDITVTVGYSGKYFGYSEI
                     DFTGITSIDADIGLAAIYTSGGVIEVRLDSPAGDLLGMYKLNQGVMEMSEKVYTIPIQ
                     PLSGFHNVYFLFKSIDGSTSQPVAVVNWVRFNE"
     gene            complement(7904..9118)
                     /locus_tag="VGA21_14185"
     CDS             complement(7904..9118)
                     /locus_tag="VGA21_14185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014774228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015506 - nucleoside:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015858 - nucleoside transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HEX9651708.1"
                     /translation="MNSKVRVQLSVMMFLQFFVWGAWFVTLSSYLTDIGFEGLFVGRA
                     YLMNNIAAIISPFFVGMIADRFFNSEKLMGFLHLLGGVVMFRATGITEVGPLIGVLLL
                     YNVCYMPTLALVNAISFNQMQNPDKEFPGIRVWGTLGWIVAGLTILLIQTSFFEGVET
                     SNIPMKMAAGVSILLGLFSFSLPNTPPKMAGEKVSVGDVLGLKALRLMKDWSFALFVI
                     CSFLISIPLAFYYNFTNVFLNESEMTGVAGKMTMGQMSEVIFMILMPFFFARLGVKKM
                     LLFGMLAWVIRYVLFALGNNEALVWMYYIGILLHGICYDFFFVTGQIYVDRKAGKEIR
                     ASAQGLFALITYGLGIGVGSEVSGRIVSAYTVNGVKDWPSIWWVPAVFALVIVIFFAL
                     SFKDKQEVDSSL"
     gene            complement(9139..10329)
                     /locus_tag="VGA21_14190"
     CDS             complement(9139..10329)
                     /locus_tag="VGA21_14190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009183904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX9651709.1"
                     /translation="MASLAILGSGFISDFYVQTLQGYRSKDRVIIAHSRDELRLKKFC
                     GRHGIPNFTTTMEEAISHPEVDLVVIGLPNYLHKEAVLLSVKHKKGVLCTKPLGRNYS
                     EALEMMKAVEEAGVFNGYLEDLVYTPKTVMSIDNIRNGSIGKVIWARSRETHPGPHSD
                     WFWDKELAGGGAILDLACHCIEIARSFIGKEIRPVEAMCWAATQVKPIEAEDHAIGLV
                     KYSNGAIGQFEVSWTFRGGLDLRDEVMGTEGTIWLNHFLRTGFELFTTSEKKGYVSEK
                     SESQKGWLFPVGDEKVDLGYVNMFTDMLNAWEEKKRPVETFYDGMVVNAIMDACLLSA
                     KTKKWEPIVIEDWRGEMDSPKDSFKQEYDADHFLVKIEKLQDGSKKIILQEKRTGKIS
                     QRVE"
     gene            10643..11887
                     /locus_tag="VGA21_14195"
     CDS             10643..11887
                     /locus_tag="VGA21_14195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014020316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX9651710.1"
                     /translation="MSLKTSPSTVVKHVLIFLFFCIGIASIAQEADSVAAASDDTEMI
                     AKGQTLFQQNCAQCHRVQAKLIGPALAGINDRRELSWIINFVKNSQKVITSGDEYANN
                     LFNEYNKVEMPAFDFFTDEDITSIISYVQTEAGKPAEVVQTSDAGQGATTAQAPTGYL
                     IAILIGLLFVIFLILIVLILIVGILKRYLDQKGDLGKADQEVVSGTIDWGKVLRSKAA
                     MGAIAFVFTAIVLKSVIDGLYHIGVQQDYAPTQPILFSHARHAGQFAIDCNYCHTGVR
                     KSKSANIPSANICMNCHTEILRDSPEIQKIYTALANDTPIEWVRVHNLPDLAYFNHAQ
                     HVQVANIECQTCHGPVEEMEVVRQFAPLTMGWCIDCHRKTDVNTRGNGYYNKLVELHE
                     KNSKEPMKVEDIGGLECARCHY"
     gene            11913..15035
                     /locus_tag="VGA21_14200"
     CDS             11913..15035
                     /locus_tag="VGA21_14200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009580628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAT-variant-translocated molybdopterin
                     oxidoreductase"
                     /protein_id="HEX9651711.1"
                     /translation="MKETKKRYWKGLEELNNDPEYLKLKDKEFPEYLPTNGSSDSQWD
                     RRDFLKLMGFSLTAATLAACETPVRKAIPYLNKPVNVDPGIANYYASTYQEGGDYCSI
                     VVKTREGRPIKINGNKLSTINNGSVTAQVEASVLSLYDTERLDGPRKGNTDLNWSELD
                     SEIIAQLNQISAAGGLIRIVSNTILSPATKEVIEIFKTKYPSTLHVVYDTRSYHGIRK
                     GNMASFATDTMPAYDFSKANVIVSFDADFLGTWLSPAEFSGQYSKNRKLGPNKKSMSR
                     HFHFESNMSLTGANADYRGKMKPSQHGLAVATLYNMIAAKTGAAAANVPAVDIPFLQK
                     AATDLWNNRTKSLVVSGSNDYAVQVLVNAINDLLGNYGKTLDLTKPINLAQGNDESMD
                     AFISEAKSGSIGAAIFLNCNPVYDHPRGSELGSSLANISLTVSTSDRRNELTSLVGYC
                     APDNHFLESWNDAEPGKGHYSLAQPTITPIFYTRQAQETLLKWAGNEMTDYLEFLQNS
                     WKTKFYPNANDSIGFQIFWDKTLVDGVMVVISEETLEQPAFSADFASVETALNQNYKK
                     DNSGWELSIYQKVGMGSGSMANNPFLHELPDPVTKVTWDNYVTVSQSDAAELGIEMDG
                     DRTTKVNLNVGNISLSVPALIQPGQAKGTLGLSLGYGRTAAGRVGNGLGVNAYPLVKL
                     LNGTLAYTISSGVQISNTEEEHRIARTQTHQTYMNRNFVVQEAKLSEYQSDPLAGRHV
                     PKIKTWSDPNHEKEPEAITLWKGHKYTNHHWGLIVDMNSCTGCSACVVACNVENNIPV
                     VGKDEVLNRREMHWLRIDRYYSSDAADDDQYALEEASENPEVTFQPMMCQHCNNAPCE
                     TVCPVAATTHSTEGLNQMAYNRCIGTRYCANNCPYKVRRFNWFKYHDNRQFSGVNTTM
                     NTDLGKMVLNPDVVVRSRGVMEKCSMCVQRIQDGKLKAKKEGRRTTDEDINTACADAC
                     PTHAITFGDMLEKESLISKMLRIEYNEDGTVKGAQEERAYHVLEEVGVRSNVHYLTKI
                     RNKDIEEENV"
     gene            15060..16424
                     /gene="nrfD"
                     /locus_tag="VGA21_14205"
     CDS             15060..16424
                     /gene="nrfD"
                     /locus_tag="VGA21_14205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HEX9651712.1"
                     /translation="MQAVSSVRVPLVTSGKTVSDVTEDICRQVEGPPTKSWLLGMAIS
                     LTALGIGGYALFMLLWHGIGVWGLNKTVGWAWDITNFVWWVGIGHAGTLISAILLLFR
                     QKWRMSINRAAEAMTIFAVICAAMFPGFHMGRLWLGFYWALPLPNTFGSLWVNFNSPL
                     LWDVFAISTYFTVSLVFWYIGLVPDFATIRDRAKTQVAKTIYGALSLGWDGAAKTWQR
                     FEAVALVLAGLATPLVLSVHTIVSMDFATSVIPGWHTTIFPPYFVAGAIFSGFAMVLT
                     LMLVTRKMFRLEDYITIEHVEMMNIIIIITGSIVGIAYITELFMSWYSQVPAEQYAFF
                     NRAAGPYWWAYATMMTCNVISPQLFWIKKIRRNLMATFILSIVVNIGMWFERFVIIVT
                     SLHRDYLPSSWAMFYPTRYDVGVYLFSFGFFFTAFLLFAKYFPVVNIAEIKSIIKSTS
                     EKSK"
     gene            16427..16954
                     /locus_tag="VGA21_14210"
     CDS             16427..16954
                     /locus_tag="VGA21_14210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013455798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3341 domain-containing protein"
                     /protein_id="HEX9651713.1"
                     /translation="MQVDKNYLVGVYSDEDVVVNAVKSVRSKGVKIHEVYTPFPIHGL
                     DHALGYKESRIHISAFLFGLTGTILALTMMFYMLGIDWPMIIGGKDHASLPPLIPVTF
                     EITVLLAALGMVGTFLVISDLKPYKKPRLFDIRITDDKHVMAVDLAKNTFFTKDQLIS
                     IIKESGAEEVNEKNF"
     gene            16986..17594
                     /locus_tag="VGA21_14215"
     CDS             16986..17594
                     /locus_tag="VGA21_14215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013455799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX9651714.1"
                     /translation="MNKIIVATFAVVLLGCRAGGNDPGLEYAPNMYHAVPYEPLAQIK
                     DKDAGNWVSSLEDGKGEYYSSNPYNPYEMNMRMPPANSVPRTAGGYLPYRIPKDSLEY
                     AARTLKSPLDSTAAITEEGKLLYVRFCQHCHGESGQGDGPVGVALAGVPAYNSALVKD
                     KPEGHIFHVITMGKGRMGAHASIVSPEERWKIVRYVEQLQKK"
     gene            17597..18955
                     /locus_tag="VGA21_14220"
     CDS             17597..18955
                     /locus_tag="VGA21_14220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009195225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol:cytochrome C oxidoreductase"
                     /protein_id="HEX9651715.1"
                     /translation="MNLDERYTFGSSTKKKLLVFAAVGVVVTIIGLIMVMSGGGEHGA
                     ENEHAATLAAKNLLADANPSGATPVADASEESHGSAPWLKRLFVGIWINNIFFIGIAI
                     IGVFFVAIQYASQAGWSAGMQRIPEAFGYWLPIGAIITLVMFFVAGHDIFHWTHTDLY
                     TEGGANYDKIISGKRAYFFWPLQDNPGFPIFFIVRMVAYFALWIWFFMMLRKHSLAED
                     LDANTSHWYKMRSLSAIFLIIFAVTSSTAAWDWIMSIDTHWFSTMFGWYTFASWWVSG
                     LAMTTLIVIYLKEKGLLSLVNENHLHDLGKFVWAFTIFWTYIWFGQFLLIYYANIPEE
                     SIYFHERMKTSNYSGIFYFNLIINFFLPFLILMTRDAKRKMTFLKLVCTMLLIGHWFD
                     FYLMITPGSLQFDGGFGLLEIGVTMIFLAGFLYVALVNIAKAPLIAKNHPMLDESRHH
                     HI"
     gene            18992..20068
                     /locus_tag="VGA21_14225"
     CDS             18992..20068
                     /locus_tag="VGA21_14225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008627759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HEX9651716.1"
                     /translation="MKVIVIAGIVLIVVILFLIFRIATLVGILKGGEKRDGQSNQINA
                     LLLLIFIIVSGSVLLWYAYTKYDEYTLPVASEHGVITDNLFWVTTLITGFVFIITQAL
                     LFYFPFKYQYKEGRKAFFYPDNNMIEMLWTIIPAIALAILIFYGLTVWNDITDEAPQE
                     AERIEILGHQFAWKLRYSGRDNLLGKHDYRLIMAENPVGVDFTDKNAFDDFSAGQMYV
                     PKGRPVELRIRARDVLHSVFAPHFRLKMDAVPGMVTSFWFVPTKTTAEMRVETGNVNF
                     NYEIACTEICGYGHFGMRMIVIVVEPKEFDQWKASQETLIQREPGLLKYVSEDLKELA
                     RIKSGLTDIQDKESESVSVAGSNK"
     gene            20076..21956
                     /locus_tag="VGA21_14230"
     CDS             20076..21956
                     /locus_tag="VGA21_14230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009580634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEX9651717.1"
                     /translation="MATAEIHIEEGHSAGGHEHDHDHQGNFWTKYIFSQDHKVIAKQF
                     LISGIFWALLGGILSVIFRLQLGFPDMNLMWLKPFLGGWITEAGKLDQEFYLALVTMH
                     GTIMVFFVLTAGLSGTFSNFLIPLQVGARDMASGFLNMLSYWFFFLSSVVMFSSLFIS
                     TGPASGGWVIYPPLSALPQAMSGSGLGMTLWLVAMALFIISTLLGGINYVTTIINLRT
                     YGMTFTRLPLTIWAFFLTAVIGILSFPVLFAAALLLIFDRSFGTSFYLTDIYINGEAL
                     PNSGGSPILFQHLFWFLGHPEVYIVLLPALGITSEIISTNSRKPIFGYRAMVGSLLAI
                     TFLSFVVWAHHMFVTGMNPFLGSIFMLLTLIIAVPSAVKVFNYLATLWKGNIVFTPAM
                     LFSIGLVSYFISGGLTGIYLGNAAVDIQLHDTYYVVAHFHIVMGSASFFGMLAGIYHW
                     FPKMFGRMLDNRLGYIHFWLTFVGVYLVFFPMHFIGMAGFPRRYYSFTNFDAFQSFTD
                     LNMLITIAAIITLAAQFIFLFNFFYSIFRGRPAPANPWRSNTLEWTTPIHPGHGNWPG
                     EIPHVYRWPYDYSKPGAKEDFIPQNIPFSDTMESNLPEENELIKLEGADSGAIKVDDT
                     KH"
     gene            21940..22995
                     /locus_tag="VGA21_14235"
     CDS             21940..22995
                     /locus_tag="VGA21_14235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008199293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HEX9651718.1"
                     /translation="MIQNTDKELAAFKKLSLITLIAVYFLILVGGIVRSTGSGMGCPD
                     WPKCFGSWIPPTSENQLPENYQDKYSMQRHEKNIKLANYLSALGMKNTAMRILDDDNI
                     RVEEEFNAVKTWTEYINRLVGATIGFLIFLTTLASFRLWKRNKVIVIMSVITLGTIGF
                     QGWLGSLVVSTKLLPGMVTTHMLLALVIVAMITFLWFKGNGNKFELKGGSLNIIIILS
                     LIALAIQVLLGTQVRERVDVAGAAAVNSGDWVNQIGLIFYVHRSFSIIILIISILLVN
                     KIVKEIGRENQHYKLSRIVAGLVILEMMTGASMAYFSIPPFLQPIHLLLGTLVFGLTF
                     LLFLSMSSEKYQIEVVK"
     gene            22992..23885
                     /gene="cyoE"
                     /locus_tag="VGA21_14240"
     CDS             22992..23885
                     /gene="cyoE"
                     /locus_tag="VGA21_14240"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014020325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HEX9651719.1"
                     /translation="MNSPGTNIIIKTGVLYRTRAFVELLKTRLSFLVAFSSGFGYILA
                     TNSFNWIQFTCLFLGGFLVSGGAVTINQILEKDVDAIMNRTKGRPIPTGRVSIPEATI
                     FSILISFAGLMLLLYSTNILTATLSLVSLILYSFVYTPLKRVGSIAVFVGAIPGALPP
                     LLGWVAATGQISVEAIMLFSIQFVWQFPHFWAIAWVSYDDYKKAGIKLLPSGGGKNLN
                     TAIQIMTYTLFLLPLGILPAKLGITGVNSALVATICGALFLAQTFSLMKDGSRKSALR
                     IMYGSFLYLPIVQIAYLLDKI"
     gene            23935..24468
                     /locus_tag="VGA21_14245"
     CDS             23935..24468
                     /locus_tag="VGA21_14245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010610201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HEX9651720.1"
                     /translation="MDPKKFILWLFIVSIIMLFGAFTSAYMVKKASGDWLSIALPGIF
                     YFNTVVILLSSASMHIAVMSTKKNNLKLTRITLVITSILGLTFLAGQYSGWQDLVRNN
                     VFFVGHPSGSFIYVLTGVHGIHLVSGVIFLFIIVAAAFRFKVHSKSQRWIQMCATYWH
                     FLDALWLYLFIFLLLNN"
     gene            24486..25253
                     /locus_tag="VGA21_14250"
     CDS             24486..25253
                     /locus_tag="VGA21_14250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019944896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HEX9651721.1"
                     /translation="MSSTTAAVSDGKKLWYGGQSPVKASYGKLMMWIFLVSDAFTFSA
                     LLITYGLIRYSHPAFEGAVREFTFSTEYWPVPEKVFAAIPFFHGLNAPLVFVGLMTFI
                     LILSSVTMVLAVEAGHRMDRKNVEKWMLWTIVGGLAFLGCQAWEWTHFIIGTGEATMA
                     KRFADGAWQSFEVLGANLSINQYGPPDFAALFFFITGFHGTHVFSGVLLNFLIFHNAT
                     TGVYDRRGHYEMVEKVGLYWHFVDLVWVFVFTFFYLV"
     gene            25274..25591
                     /locus_tag="VGA21_14255"
     CDS             25274..25591
                     /locus_tag="VGA21_14255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009580639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HEX9651722.1"
                     /translation="MEETKEIIVQPVNKEKVRQLVRVAIILGIVTGLEFLVAFTFHSG
                     PLKTSIFVAMTIIKAFYIVGEFMHLRHEVKMLIWSILLPMMFVVWLLITLIYEGGSIL
                     QVR"
     gene            25656..26198
                     /locus_tag="VGA21_14260"
     CDS             25656..26198
                     /locus_tag="VGA21_14260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651723.1"
                     /translation="MKKFVLLVVVIIPVGLVLFVKFFGKNEYNLPVYYQNGYPAITDC
                     TLVGAAVLEPAGLAFENLNRVNLIFYEPECESNLDFFNSQTERIRLNMSEADFINYIT
                     ISSVDSTYSQEVRKKFAVCGLLIAEQKEENLKDWKCTGFIVMIDWKARIRGYYSGTDF
                     EDIDRVILELNVMETIVNNE"
     gene            26191..26715
                     /locus_tag="VGA21_14265"
     CDS             26191..26715
                     /locus_tag="VGA21_14265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020530646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF420 domain-containing protein"
                     /protein_id="HEX9651724.1"
                     /translation="MNSKNRPIWIIISTSIVIPVLVAILFFSEGIIPLLGDWVYSLPQ
                     VNAVINSVTAILLIAGKYQIRMGNKELHKRLMITSFILGIVFLLCYVTYHASVPSTHY
                     QGEGFLKYIYFFLLVTHIITAIIVVPFVLLALFYALQENFFRHRKIVKYAHPLWLYVS
                     ITGVIVYLMISPYY"
     gene            26737..26973
                     /locus_tag="VGA21_14270"
     CDS             26737..26973
                     /locus_tag="VGA21_14270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014020331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651725.1"
                     /translation="MKRVLLLVIALIVSYGTSFAQCAMCRATLETSVSNGESLEMAST
                     LNFAILYLLALPYLLALVIGLLWYRRARGKGTAS"
     gene            27020..30736
                     /locus_tag="VGA21_14275"
     CDS             27020..30736
                     /locus_tag="VGA21_14275"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX9651726.1"
                     /translation="MNRSLLFLVGLVLILSGILIKYFLLPKEAEPQEIANRIEKNLQT
                     EISISKSQLAVVRERIMNDDYLRFEDLNTIASDYPFYVYYRTRLFYWSEFQLVPEYNE
                     FSDVGTIGFIHLDQGNFIVTRDQFNKKDDIYEIVSLIPVQTEYEIFNKYLESDYNDRI
                     IEFPDMIISNSGNEGYPVKFDGQILFYVDSYISVNQYSIHFQLISISLYTIGLILILY
                     TVYSKVLELTNRRKFEIALAILFISLAGVRFVMLFTEFPSAYANWVLFDSRLFASSIL
                     NPSIGDLMLNTLALFALILFAYMNYYRSNLFIKILGLKIIARWILSVKLILIGFLAMV
                     YQFLIFRLIYENSQWSLDITSTLYFPASKVIFYLIFVINSAIFFLTFHLIFRIFTRVN
                     DKSLYSLIINYSVGALIFILVAYNLNLPIVFIIMVNLGYFLVLHFLKLSRFLVQMRYL
                     AFLYLFVAAQACALIGAYSVQYFEEENELENKFRFADQFLVESDNLGELLLSEAGFQI
                     KQDPLITSRMYSSFMSKETISQKIKRVYLGSYFDKYNVQVNLYNATGQPFNSEGTQGT
                     YRQIKDAFAQEQYETEFPNLYFVNRVGADVTKRYLYFIDIERYNQVIGYILIDLSLKK
                     IIPESVYPELLVDSRFFQPYQESEYSYAVFIGGRATYSTGNYNYTVGFDNDLLENPSL
                     FNRGLSYRGHHHFAVKDLDSRTIVITSTLYPAINVVSNFSFLFLILVAIIILIIVFYS
                     IYNWLNDVKLKFATKIQLYINLAFIIPLFVVSIATLSLINKSFRKELEIEYYKKAENL
                     GRNIIGDLNEYLKLDLDREDLGNRLASITANANLDANLYNIRGKLIGSSQPSIFDKKI
                     ISEYINPKVIELIREDGNSEAILRERIGSLTYNAAYVALKSFNDGSVLGILNIPFFDS
                     ETIVERQQIDVLSNIMNIFTAIFLILLFLSYLASKWLTLPLQMITQRIRKISLSGENK
                     PLAWNSEDEIGLLVGEYNRMLINLEVSKKALAKSEKESAWREMAQQVAHEIKNPLTPM
                     KLTLQQLEKSLEELKLQKGSTEKPIKTLLYQIDNLSDIASSFSAFAKMPIPEMKNIEL
                     TSLLRSTFDLYKNQDKGKVTLNQYHRELYVLGDDQLMGRIFSNMIINSLQSSNDDDLV
                     TVVGTIHVNGKGNVLITIKDDGNGIPMEIRDKIFVPNFSTKTNGSGIGLAIAKHGIEN
                     MGGKIWFESEIGIGTTFFIEIPLDSKGSFLVGSH"
     gene            complement(30713..31459)
                     /locus_tag="VGA21_14280"
     CDS             complement(30713..31459)
                     /locus_tag="VGA21_14280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019945891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX9651727.1"
                     /translation="MIEIRNIHKSFEGKTVLTGIDGIFKRGTTSMIIGASGTGKSVLL
                     KCIVGLLKPDIGEVFYNEREFTNAHRNLKTEIRREIGMLFQGGALFDSKNVEQNVKFP
                     LDILTKMPEHEKIDRVNFVLSRVGLENINKKMPSEISGGMKKRVGIARAIVNNSKYLF
                     CDEPNSGLDPLTAIKIDDLIHEITQEYSITTVVVSHDMNSVMEIGEYVMFLHEGAKLW
                     EGSNQDIDKTDIPELNEFLFPNKLMRAHKK"
     gene            complement(31456..32193)
                     /locus_tag="VGA21_14285"
     CDS             complement(31456..32193)
                     /locus_tag="VGA21_14285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018477386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9651728.1"
                     /translation="MGLLSAIGKYFIFLGSLLINRESFRTYLKLVFDEAILIGINSIV
                     LVSLVSLFIGAVTAIQTAYNLVSPLIAKYVISMVVRDMTVLELAPTITAVVFAGKVGS
                     SIAGGLGTMRITEQIDALEVMGINSVSYLVLPKILATLIMYPLLVVLAGTLSIWGGYI
                     AGTLSGVVTPEEYIFGIRASFVEYTITFALIKSYTFAFLISSISSFKGFYTEGGALEV
                     GQASTSAVTNSVIAILVADYLLAQLLL"
BASE COUNT         9568 a         6240 c         6794 g         9754 t
ORIGIN      
        1 tacggaaatt taatccgtac ttgatcccca atttacgaag acttaatcct gtggataaat
       61 attcggcaat gatactttct tttaattcta tgtgattcat tttggttgac tttattgtca
      121 acctatttca ggacgagaca accattttaa gaaccaccct gcccgcaaat tgtccccctt
      181 attttatggg gactacaggg ggtataagtt ttgttcggga acgcttcggg caagagaagg
      241 atcaagaatc aagaggctta ttctatgaaa ttgaatcagt cccctcaaac tcgaatagta
      301 agagtcgttt gaagagttca attggcatga gtcctaattc tcttcgtcta tcgtccaatt
      361 tttcctcatc agtaattggc atcaatacgg ctttacgatc agcgtaatca ggtgcagaaa
      421 aatgagtacc gtaaacttgt ctttcaccgg cattcaattt aaccctatcg aaaagatagg
      481 cgtaatcttc cgtgcttaat tctccttgtt cagcggcaag tttcacattt ttcaattgtg
      541 atgcctgaaa ctccggatac ttattggcat ttctaattat agtatatata ctcatcatgc
      601 cctgtttgcc taccattttt ctgtcaggaa agccgatctc atcaataata attttcaatt
      661 tttcaatatt gattttgtca gtcccatcaa tatcttcatc ataaatttta tccttgctaa
      721 tactgtaacc tgtttcatta accaggctcc tgatttctcg cccgcgggca atttgatcat
      781 taaaatacat tattaacaag tcttctttta tttgggggaa caggagatct gcgttattat
      841 ccatcctgat gcaaatgtct ccccactccg gagtatttgg tggcctaaac caaccttcaa
      901 cacacaaatt gatttcaaaa atgcgtggat tattgagaag aacaacatac gcttttggat
      961 attggtttgt ttcggccagg caacgaatcc cgctaataag taaatttgga tcaatattgg
     1021 atttaatagc caacaagctg tcaaattgtt tagcgccaag tgcatagttt ttgtccaatg
     1081 attctgattc ccacattctc caggctagtc tccattccac ttcattttta tacaagttgt
     1141 ccctgcaccc tgcgaacatt attaacagta agcaaccagt gatagttcta cgataagtaa
     1201 cttcaaacat cattcatgga aaattaggta tgctgcaaat gtaagaaaca aaatggtttg
     1261 gagttaaagg taggtgtgat gaaatgaact taaaacctga taattttccg tataagaaat
     1321 tggggctaat cgtaaccttt ttctcattgg atcagtctat atatataatt tacatatata
     1381 taaaatatga aaggaacata tcttggtgaa tttgaggagg ttatattact aattgtagca
     1441 attcttaatg gcaaagctta cggaaactcc ttaaaagaag aatttcaggg acgcgcaaac
     1501 aggtcggtga acctgagcgc aatacatgcg gctttataca ggttggaaca aaaaggattt
     1561 gtcaaatctc agtttggtga acctacctgc gaaaggggag gcaaaaggaa gcgattcttt
     1621 tcggcaacca acaaaggctt gtcagccctt caggaatcaa gggacttgcg taaaaatctt
     1681 tgggctgatg taccggttaa tgcgttaagc aaacttcgat gaataagcct ccgaaaattg
     1741 caacctggtt tctacggtta ttctgtgatc ccgcatttgt agaaatgatt gagggagatt
     1801 tgtatgagga atttcaaact cgcttaacct ctcgtggaaa gaagaatgca actctctatt
     1861 attggtggag tgttattcgt tctttccggc cttacctttt aaaaagaaat tcaaatccat
     1921 ataaatacac tgaaattatg ctgcttcaca actacttcaa gatagcctac agaaatctga
     1981 tcaaacagaa actgtatact tcaattaatg taattggatt gactgcggga atggcttcca
     2041 gcttgttaat tcttttgttt gtaaaatctg agctcacctt tgatcaacat catgaaaggg
     2101 cggaggatgt ctttcgcgtt tccacaaatg gagaatctaa tggagaaaca atgaatatgc
     2161 aaaccagtcc tttgggattg gctttcaaaa tgaaatctga atttccggaa gttgaggaaa
     2221 ttgctcgttt gtccagcttt gtgggggttg agaaaaattt ggtttcttat gaggaaacaa
     2281 aattttttca ggatgaaggc tacctggcag attccgcatt tttcaaaatt ttgaaatatg
     2341 attttgcaga gggaaatccc gccacctgcc ttaacaattc cggaaacata gtaatctccc
     2401 ggtcgcttgc tgaaaaaatt gccgggaatg aacctgcatt gggaaaaatc atcagcatcg
     2461 aaaactcttt tggtaaagtg gattatgaaa ttaccggtgt ttttaaagag ccggttaaaa
     2521 atacccatct taaaatttcc tttgtaacga gcatgaacaa tcctggcatt ggagaatata
     2581 ttttcagaag taatgaattg ctcggaaata acttcgtgta ttcgtacctg cgtttaaatc
     2641 aaaatgcttc tccgcagcaa ttggtatcaa agtttcctgc ttttttagat aagtatgtgg
     2701 gtgaagacat ggaaaagacc ggatttcgaa aatcccatga cctgattaat gtggcagaca
     2761 ttcatttgaa agcaaaagga ctgggctttc aaaatgaagg tgatttgaag actatataca
     2821 tcttaagcac cattgcaggg ataattctgc taattgcgtg tattaacttt atgaatatgg
     2881 ccactgctca gtccgggcac agaagcaggg aaattggagt gagaaaagtt tttggggcgc
     2941 aacgaatttc cctggccaat caatttctta cagagtcagt tcttatcaca ttgattagct
     3001 taattgtgag cattggaata gtatatttac tgctcccgtc ttttaaccag ataacagggc
     3061 agagaattac ccttgatttg cttgaaccag cacaactgct gattgcagtg gcaatcggct
     3121 ttatcacggg atttattgcc ggatcttacc ccgcatttca tttatcagga atatcgataa
     3181 acagaatctt taaaaataag agtaacaaat caggcttagg aatggtgaga aaaggcctgg
     3241 taatttttca gtttttgata tcaatcagtt tgattgttgg ggtgataacc atcaatcaac
     3301 agctgaattt tgtgcagtca agatcgctgg gtttccaaaa ggaaaaccaa attgcaattc
     3361 ccttacgctc gcctgaggtt ggacgacaat acaaaattct ggagaatgaa ttttcaaaac
     3421 ttcctggaat agtaaacatt ggtgccggcc acgcttatcc cggaaaattc gttttcaatg
     3481 ataattccta ttacaaggat ggaagttcaa tggatgaggc ggtaaatatc cgcacaaatt
     3541 atgtggattt caagttcatg aatactctgg gtgtagaagt tgtaaatggt cgggaatttt
     3601 cagaagattt ccccgcggat gttaacacag cggtagtctt aaatgaagcg gccgtacgca
     3661 atttgggcta tgaattggat gatgttgttg gaaggaacat acattccatg ttccctatta
     3721 atggaaggga tactattata acatacaaag tttcaggtgt cgtaaaagat tttaattttc
     3781 aatctcttta tgaagacatt attccatata tgtttacgat gtcaaatcct gatacttttc
     3841 aacccaggta tttgctggtt tctgtaaaca caaataatta tcaaaacttg tttgcagaaa
     3901 tgaaaactac ctgggataga caggccggtg ggataccatt tgaatatacc tttatggatg
     3961 atgatctgaa ttctcagtat aagtccgatg aaagtctctc atttatcgtg aaagtattta
     4021 caacagtcgc catcattatt tcagcactcg gtttgttcgc tctctcgtca tttactgcgg
     4081 aaagcagact gaaagaaatg ggggtgcgaa aagtaatggg agcatccggg aaggatatct
     4141 ttttgatcat gtcaggaaat ttttcaattc tcatcctgat ctcttttgta ctcagcatcc
     4201 cgccaggctg gtacttcatc agaacctggc tcaattcctt tgccttccgt attgatataa
     4261 acccctggat attcctattg gcaggcgctg taactttcgc ttttgccatt ctggtggtca
     4321 gctatcaatg cctcaaagtt tcattggtga atccggttaa tgtgcttaaa gacgagtaac
     4381 aggtatcagg gaatctcagg caaagatcat cagtaagtga tcatttttta ttacgatttg
     4441 tatttgactt gatagttcac catttgttgc cttacttgtg cttcaaacaa atcgtatgcg
     4501 caaactaatc attattattt ccttgattat tctggcgaca ctgctttatc ggtggttttt
     4561 tagaaggcca caatcttgta atgttttagt tttttcgaaa accgcggaat tccggcatga
     4621 gtctattgaa gagggtaagc ttgctatcat gaagctgggt aaagaaaacg gattcgaagt
     4681 ggatacgaca gaagatgctg ccattttcaa ggaaagaaac ctgcgcaatt ataaagtgat
     4741 aatctttcta tctacaacag gggatgtcct taatgaatct cagcaattag aattaacacg
     4801 ctttattcag gcgggtggct cgtttgtggg tattcacgcc gcggtggata cagaatatga
     4861 ctggccttgg tacaatcaac ttgttggtgc ttattttgaa agtcatcctc aaattcaaaa
     4921 ggcaacggta aaagtggtga atccacagca tcctgcaaca gaaaggttac cgacagaatg
     4981 ggagcgggtt gatgagtggt acaatttgaa agatatccag ccctacatta atgtgttaat
     5041 taatctggaa gaatcctctt atgagggcgg gaaaaatggt gagaaccatc ctatctcctg
     5101 gttccatgag tttgatggag gccgggtttt ttatagtggg ttcggtcaca cctccgaaac
     5161 ttttcaagag cctgaatttc tgaatcatct tctgggtgga attcaatacg ctgccggcga
     5221 tggttctccg gttaattatg ataatgtaac tgtaactccg gatgccagaa ggtttgcgaa
     5281 aacggttatg gaatcaggat tgaatgaacc tatggaactg gaagttctca aggatggcag
     5341 agtactttat attgaaagaa ggggcgggat tagaatgttt tttcctcagg agaataaagc
     5401 gaaaacaata ggcagacttg aagtattttc agagcaggag gatggcttgt tgggattagc
     5461 caaatctccg gactttgaat caacaggatg gatctattta ttctattctc cggcgattga
     5521 tgatcatgtc cagtatgttt cacggttcac aattatagat gataccgtag atctatcctc
     5581 ggaaaaaata attctcaaaa tacctaatca aagggatgaa tgttgccact caggaggcgg
     5641 cctggaattt gatagtaaag gcaatttata cattacagtc ggcgacaata caaatccatt
     5701 tgcatcggat ggttacgcac ccattgacga gcgggaggga cgcagcgcgt gggatgccca
     5761 gaaatcatca gcgaacagca tggatctgcg tgggaaaatt ctgcgaataa aaccggaggc
     5821 ggatggcact tattctatac cagacggaaa tctgtttcct aaagacgaca ttaatagcag
     5881 gccagaaatt tatataatgg gaaaccgtaa tcctttccga ctttcggttg ataacaaaac
     5941 cggatttatc tattggggtg aagtaggtcc ggatgccgct aaagacagca cagatcgggg
     6001 accgaaaggt tatgatgagg tgaaccaggc ccgtagcgca ggtttttttg gctggccata
     6061 ttttgttgcg gataacaaag cgtataatga ttatgatttc acgaaaaacc tttccggaag
     6121 aaaatttgat gcgcgtaacc cgataaacaa ttcacccaac aattcaggac ttggtaaatt
     6181 gccatccgcc cagcctgctt ttatttacta cccatatgat gcatcggaag aattcccgct
     6241 gaccggaacg ggtgggagga atgcaatggc aggacaagta tattattttg aagactacaa
     6301 aaattttgca aatgcatttc ctgaatatta tgatggtaaa ctattcatct atgactggat
     6361 gcgtgggtgg atcaatgttg taactatgga tgatccgggt aattatacga aaatggaagg
     6421 ttttcttccg gaagaaaagt ggattcaccc cattgatatg gttttctcgg aaaaaggaga
     6481 attatatatt attgaatacg gctccatttg gtatgtaggc aacgcggatg cccggttgtc
     6541 gaagatcagt tttatctcag gaaacagggc ccctgttgcc tccattacca ctgataaaat
     6601 agccggtgct gtgccattag aggttctcgt ttcagcgcgg aacagcgtag attatgatgg
     6661 cgatgatctc acttaccact ggaagttgga tggtaagtta gttccgggaa ttagcggtga
     6721 tgagaactta accatttctg aaaacggaat acacgaagtt gaactaacgg ttacagattc
     6781 agaaggtaat gtaagtaaaa atagcttaga tatatatgcg ggaaatacgc ctcctgaaat
     6841 tgcgttggaa attgcgcaga atgagacatt tttcaggggt ggagatttga aatactccat
     6901 taatctgaca gatgaggaag attcccaaaa taataaaata gatctgtcaa gattggtaat
     6961 tgccggcacc tttctgcctt caggttttga gcttaaaaat actttatctt tccaggatta
     7021 cctgaataac aacccggcct acctggggaa aattctaatt gaaaattcag attgtcaggc
     7081 ttgccaccag gaaagacaaa gttcggttgg accatcttat ctggagatca gcaaccggta
     7141 tgaaaataat gagctaaaca gtaaatcttt atctggaaaa atagtgaatg gcagcgtggg
     7201 agtttggggt acgagaccta tgattgctca ccaccaactt aatgaaacag aaacaaaatt
     7261 gatggtggag tatattctga atctggataa accggtaaag caatctgaaa cacaattgtc
     7321 cggaattttt gaacttaaag agcaaagtga aaaaggtata tatgtgatct ctgcttcata
     7381 cacggaccag ggtgcaaacg gaatacaacc tttgacaact cgtgattata tgattttgag
     7441 gaatccggtt ctggaagcgg aagattttga tttctctaac cagatcatga cttattctgc
     7501 caaagcaggc gaagttccaa tcctcgatcg cgacattaca gttactgttg gctactctgg
     7561 aaaatatttt ggctacagtg aaatagactt taccggaatt acatcaatcg atgccgatat
     7621 tggcctggcg gcaatctata ccagtggcgg cgtaattgaa gtgcgattag attcccctgc
     7681 cggtgacctt ttgggaatgt ataagcttaa tcaaggtgta atggaaatga gtgaaaaagt
     7741 ttatactatc ccgatacaac cactttccgg atttcataac gtttatttct tgttcaaatc
     7801 tattgatgga agtacaagtc aacctgtagc ggttgtaaat tgggtgcgtt ttaatgagta
     7861 attgctcaga aatccgatgg gaagagacag tctctatttt ttattacagt gaactatcca
     7921 cctcctgctt atccttaaag cttaatgcaa aaaagatcac aattaccaat gcaaatactg
     7981 caggcaccca ccagatagat ggccaatctt tgactccatt aactgtgtaa gcggaaacaa
     8041 ttctgccgga cacttctgaa ccaacgccga ttccaagtcc ataagtaatc aatgcgaata
     8101 atccttgagc gctggctcga atctctttac ctgctttcct gtcaacataa atttgacccg
     8161 ttacaaagaa gaaatcataa caaattccat gaagcaaaat tccaatgtaa tacatccaaa
     8221 ccaaggcttc attattaccg agggcgaaca aaacataccg gatcacccag gctaacattc
     8281 caaacagcag catctttttt acaccaagcc gggcaaagaa gaacggcatc aggatcataa
     8341 aaattacctc cgacatttgg cccattgtca tctttccggc tactccggtc atttcacttt
     8401 catttaaaaa tacattggtg aaattgtagt agaatgccag gggaattgag atcagaaaag
     8461 aacaaatgac gaaaagggca aacgaccagt ctttcatcaa ccgtaatgct tttaaaccaa
     8521 gaacatcgcc cacagaaact ttttctccag ccatttttgg aggtgtattt gggagcgaaa
     8581 aagaaaataa ccctaaaaga atggaaacac ctgccgccat tttcatgggt atattcgaag
     8641 tctcaacacc ttcaaaaaag gaagtttgaa ttaaaagaat agttaaccct gcaacaatcc
     8701 agcccagagt tccccacact cttatccccg gaaattcctt atcaggattt tgcatctggt
     8761 taaatgagat ggcattcacc agggctagcg tcggcatata acacacgtta tagagtagga
     8821 gtactccaat cagtggtcca acctcagtaa tgcctgtcgc ccggaacata accacaccgc
     8881 ccaataagtg caaaaatccc attaactttt ccgagttgaa aaacctgtca gcaatcattc
     8941 ctacaaaaaa gggggaaata attgccgcaa tattattcat gaggtaagcc ctgcccacaa
     9001 acaaaccctc aaagccgatg tcagtaagat aagaggaaag agttacaaac caggctcccc
     9061 acacaaagaa ttggaggaac atcattacag acaactgtac tcgtactttt gaattcatca
     9121 ataccataat atatagtgtt attcaactcg ttgggaaatc ttaccggttc gtttttcctg
     9181 aagaattatc ttcttacttc cgtcctgcag tttttcaatt ttcaccagga aatggtctgc
     9241 atcgtactcc tgcttaaaac tatctttcgg ggaatccatt tcaccccgcc aatcctcgat
     9301 tactatcggt tcccattttt ttgttttggc agataacagg catgcatcca tgatggcatt
     9361 caccaccatg ccgtcataaa aagtttctac gggtcgtttc ttttcttccc acgcattaag
     9421 catgtcggta aacatattta cgtagcccag atcaactttt tcatctccga ccgggaaaag
     9481 ccagcctttt tgagattccg atttttcaga aacatatcct tttttttctg aagtggtgaa
     9541 gagttcaaat ccggtacgca aaaaatggtt cagccagatg gttccttccg tgcccatcac
     9601 ttcatccctc agatccagcc cgcctctaaa cgtccagctt acttcaaact gtccaattgc
     9661 tccgttggaa tacttgacga gtccgatggc atgatcttcg gcttcaatag gttttacctg
     9721 ggtagccgcc cagcacatag cttccaccgg tcttatttcc ttaccaataa aactcctggc
     9781 aatctcaatg caatggcatg caaggtcaag aattgctccg ccgccggcga gttctttatc
     9841 ccaaaaccag tcgctgtgcg gacccggatg tgtttcacga ctcctggccc aaattacttt
     9901 tccgattgaa ccattgcgga tgttgtcaat tgacatcacc gtcttcgggg tataaaccaa
     9961 atcctccagg taaccattaa acacccctgc ctcctcaact gctttcatca tttccagcgc
    10021 ttcagaatag ttacggccca gaggtttagt gcagagaact ccctttttgt gctttacact
    10081 aagcagaacc gcttccttgt gcaaataatt aggaagacca atcacaacaa gatccacttc
    10141 gggatgactg atggcttctt ccattgtggt ggtaaagttt ggtataccat ggcggccaca
    10201 gaatttcttc aaccttaatt catcacgaga gtgagcaata attactctat ccttgcttcg
    10261 gtatccctga agtgtctgga cataaaaatc gctgataaaa cctgacccta aaattgcaag
    10321 acttgccata taatgtgtcg ttgaatatat aaagagattt gattaaacaa aaacattgac
    10381 caggcctgac ttcagcaata atcaggaagt atgttgtttt gaaatttaaa accaatacat
    10441 ttacgagaac caaaagtaaa attcaaataa taatttatag gaagaaaaga ggatttggat
    10501 ttttaatttc agtcagaaat taggattgat ttggaccaaa tttggtcaat ttgaaaatga
    10561 ttgaaataaa tttgaaatat atatatcgaa tattagattt gtacggtctt aaaaacctta
    10621 aaccaaacta taacctataa acatgtccct taagacatcc ccctccaccg tcgttaagca
    10681 tgtacttatt ttccttttct tctgcatagg catagcttca attgctcaag aggccgattc
    10741 ggtcgcagct gcttctgatg atacggaaat gattgcaaag ggtcaaacac tatttcaaca
    10801 gaattgcgca cagtgtcacc gggtgcaagc aaaactaatc ggcccggcat tagccggtat
    10861 taatgaccgc cgggaactct cctggattat caattttgta aagaatagtc agaaggtaat
    10921 tactagtgga gatgagtatg caaacaattt atttaatgag tacaacaagg ttgaaatgcc
    10981 tgctttcgat ttctttactg atgaggatat aacttctatt atttcatatg ttcagactga
    11041 agccgggaaa cctgctgagg tagtccaaac atctgatgcg ggacagggag ctactactgc
    11101 tcaagctcca accggatacc ttattgcaat cctgatcggt ttgctatttg tgatattcct
    11161 gattctgata gtgttgatcc ttattgtagg aattctaaag agatacctgg atcaaaaagg
    11221 tgatttgggg aaagctgatc aggaagttgt ttccggaaca attgactggg ggaaagtact
    11281 taggtcaaaa gcagccatgg gagctatcgc atttgtattt accgctattg ttttgaaaag
    11341 tgtaattgac ggcctttatc acattggggt tcagcaagac tatgcgccaa ctcaaccgat
    11401 tctgttttca cacgccaggc atgccggaca gtttgccatt gattgcaatt attgtcacac
    11461 cggagtaaga aaaagcaaaa gtgccaatat tccatcagca aatatttgta tgaattgcca
    11521 cactgaaatt cttagagaca gccctgagat tcaaaaaatt tatacagccc tggctaatga
    11581 cacacctatt gaatgggtca gggttcataa ccttcccgac cttgcctact tcaatcatgc
    11641 acaacatgtc caggttgcaa acattgaatg ccaaacctgt catggtccgg tagaagaaat
    11701 ggaagttgtc cgtcaatttg cacctttaac tatgggatgg tgtatcgatt gccacagaaa
    11761 gacagatgta aatacccgcg gtaatggata ttataataaa ctggttgaat tacatgaaaa
    11821 gaatagcaaa gagccgatga aggttgaaga tattggaggt ctggaatgcg cccgatgcca
    11881 ttattaattg ataaagcgac ttactactaa ttatgaagga aactaagaag aggtactgga
    11941 aaggattaga ggagttaaac aacgatccgg aatacctgaa gttaaaggat aaggaattcc
    12001 ctgaatacct tccaaccaac ggttcttcgg attcgcaatg ggatcgcaga gacttcctca
    12061 aattgatggg cttcagcctg acagcagcaa cgcttgccgc ttgtgaaaca ccggtcagaa
    12121 aagctatccc ctatttgaat aaaccggtta atgttgatcc tggcattgct aattattacg
    12181 cttcaacgta ccaggaaggt ggagattatt gttcgatagt agttaagaca agggaaggca
    12241 gacctatcaa gatcaatggc aacaaacttt caacaataaa caatggttct gttactgcgc
    12301 aggttgaggc atctgttctt tcattatatg atactgaaag acttgatggc cccaggaaag
    12361 gtaacaccga tctaaattgg agtgagttgg atagtgagat tattgcacag ctaaatcaga
    12421 tttctgctgc cggtggcctg ataagaattg ttagtaacac gatccttagc cctgctacca
    12481 aagaggttat tgaaatattc aaaacaaaat atccctcaac tctgcatgtt gtatatgata
    12541 cacggtcata ccacggaata aggaaaggaa acatggcatc atttgcaaca gatacaatgc
    12601 ctgcatatga tttcagcaaa gcaaatgtaa tcgtatcctt tgatgcggat tttctgggta
    12661 catggttatc acctgcggaa ttttccggtc agtattcaaa aaacagaaag ctgggaccca
    12721 ataaaaagtc gatgtcccgc cattttcatt ttgaatcgaa catgtctttg acaggtgcga
    12781 atgctgacta tcgcgggaaa atgaaaccat cgcagcatgg tcttgccgtg gctacattat
    12841 acaatatgat tgccgccaag acaggcgcgg ctgctgcaaa tgtaccagca gtagatattc
    12901 ccttcctgca aaaagcagct accgatctct ggaataacag aaccaaatcc ttggttgtct
    12961 ccggttcaaa tgattatgcc gttcaggttt tggtgaatgc catcaacgac ctgcttggca
    13021 actatggcaa aaccctggat ctaaccaaac ctataaacct cgcccaggga aatgatgaat
    13081 caatggatgc ctttatcagc gaagccaaat caggttctat tggtgccgca attttcctga
    13141 attgtaatcc tgtttacgat cacccgcgag gttcggaact tggatcatca ctcgcaaata
    13201 tttccttgac agtttcaacc tctgatagaa gaaatgaatt gacatccctg gttggatatt
    13261 gtgcacctga taatcatttc cttgaatcct ggaatgatgc ggagccagga aaaggacatt
    13321 acagccttgc tcagccaact atcacaccca tattttatac ccgtcaggcc caggaaactc
    13381 ttctgaaatg ggccggtaac gaaatgactg attatttaga attcctgcaa aatagctgga
    13441 aaaccaaatt ctatcctaat gccaatgaca gcataggatt ccagattttc tgggataaga
    13501 cacttgtgga tggggttatg gtagtaattt ctgaagaaac attggagcaa cctgcattct
    13561 ctgctgactt tgccagtgta gaaacagcct taaaccaaaa ttataaaaaa gataactcag
    13621 gctgggaact gtccatatat cagaaggtag gaatgggtag cgggagcatg gccaataatc
    13681 ctttcctgca tgaattacct gatccggtga ccaaagttac ctgggataac tatgttacgg
    13741 tttcacaatc tgatgccgcc gagttgggaa tagaaatgga tggtgataga actactaaag
    13801 taaaccttaa cgttggcaac atttcacttt ctgtgccggc acttattcag ccaggccagg
    13861 caaaaggaac gctggggctt tcattgggat atggtagaac tgctgcaggc cgggttggaa
    13921 acggattggg agtgaatgct tatcccttgg taaaattgtt gaatggaaca ctagcgtaca
    13981 ccatttcatc cggagtgcaa atctccaata cagaggaaga acataggata gcccgcaccc
    14041 agacgcacca gacatacatg aatcgcaact tcgttgttca ggaggcgaaa ctttcagaat
    14101 atcaaagtga tcctttggca ggcagacatg ttccgaaaat taaaacatgg tctgatccca
    14161 accatgaaaa agaacctgaa gctatcaccc tttggaaggg tcataaatac accaaccacc
    14221 actggggatt gattgtggat atgaactcct gcaccggttg ctcggcgtgt gtcgttgcct
    14281 gcaatgtcga aaacaatatt cccgtagtcg gcaaagatga ggtattgaat aggagagaaa
    14341 tgcactggct ccgcattgac agatattata gcagtgatgc tgctgatgat gaccagtatg
    14401 ctcttgagga agcatctgaa aacccggaag ttaccttcca gccgatgatg tgtcaacatt
    14461 gcaacaatgc tccttgtgaa actgtttgcc cggtggctgc aacaactcat agcactgagg
    14521 gactcaacca gatggcatat aatcgttgta ttggcactcg atattgtgca aataactgcc
    14581 cttataaagt aagaagattc aactggttta aatatcacga taacaggcaa ttctcgggtg
    14641 ttaatactac catgaatact gatttgggta agatggtcct caatcctgat gtagtcgtga
    14701 gatcaagagg agtgatggag aagtgctcta tgtgcgtgca aagaattcag gatggaaaat
    14761 taaaggcaaa gaaagaaggt agaagaacaa cagatgaaga tattaataca gcgtgtgccg
    14821 atgcttgccc tacgcatgca ataacctttg gtgatatgct ggaaaaggaa agcctaatct
    14881 ccaaaatgtt aaggattgaa tataacgaag atggaacggt taaaggagcg caggaagagc
    14941 gtgcgtatca tgtacttgaa gaagttggag taagatcaaa tgttcactat ctgacaaaaa
    15001 tcagaaataa ggatatagaa gaagagaacg tgtaaataat aaaacgaaac aggattacaa
    15061 tgcaagcagt gtcttcagta agagtgcctt tagttaccag cgggaaaact gtttccgatg
    15121 taactgagga tatttgtaga caggtcgaag gacctccgac aaaatcctgg ttattgggaa
    15181 tggccatctc actaacagcc ctgggaatag gaggatatgc tttgtttatg ttgttgtggc
    15241 acggcatcgg tgtctggggt ttgaataaaa cagttggctg ggcctgggat attaccaact
    15301 tcgtatggtg ggtcggtatc ggtcacgcgg gaacactgat ttctgcaatc ctgttactct
    15361 tcagacaaaa atggagaatg tcaatcaaca gggcggctga agccatgaca atcttcgcgg
    15421 taatttgtgc cgccatgttc cccggattcc acatgggccg actatggtta ggcttttatt
    15481 gggcattgcc tttacctaat acattcggct cattgtgggt aaactttaac tctccgttgc
    15541 tatgggatgt atttgccatc tctacttact ttacagtttc ccttgttttc tggtatattg
    15601 gtttggtacc tgattttgca acaattcgcg atcgggctaa aacccaagtt gctaagacaa
    15661 tttatggtgc gttaagtctg ggctgggacg gcgccgcaaa aacctggcag cgtttcgaag
    15721 ctgttgcatt agtcctggcg ggcctggcta cgccactggt actgtctgtt cataccatcg
    15781 tttctatgga ctttgctact tcggttatac ccgggtggca caccactatc ttcccgcctt
    15841 actttgtggc aggtgcaata ttttccggat tcgcgatggt gcttacacta atgctggtaa
    15901 ctcgaaaaat gttcagactt gaagattata ttaccattga acacgtggaa atgatgaaca
    15961 ttatcatcat aataacgggc tccatagtcg gtattgcata tattactgaa ttatttatga
    16021 gctggtattc tcaggttcct gccgagcaat acgccttttt taacagggct gccggaccat
    16081 actggtgggc ttatgcaaca atgatgacat gtaatgtgat ttcaccacag ttattctgga
    16141 ttaagaagat tagacgaaat ttaatggcaa cattcattct aagtattgtg gtaaatattg
    16201 gaatgtggtt tgaacgattt gtaattattg taacctctct gcacagagat tatctgccat
    16261 caagttgggc aatgttttac ccaacaaggt atgatgtggg tgtatactta ttttcatttg
    16321 gattcttctt cacggcattc ttattgtttg ccaaatattt cccggtggta aatattgcag
    16381 agatcaaaag cattattaaa tcaacctcag aaaaaagcaa gtaaatatgc aagtggataa
    16441 gaattactta gtgggggttt acagtgatga agatgttgtt gttaacgcgg ttaagtccgt
    16501 aagaagcaaa ggtgtgaaga ttcatgaagt atatactcct tttccaattc atggccttga
    16561 ccatgctttg ggttataaag aatcgagaat ccatatttct gcatttctct ttggtcttac
    16621 aggaacaatc ctcgccttaa ccatgatgtt ttacatgctg gggattgact ggccaatgat
    16681 tattggagga aaagatcacg catcattgcc tccgctcatc ccggtaactt ttgaaataac
    16741 agtgcttctg gccgcattag gaatggttgg aactttcctg gtgatcagcg atttgaaacc
    16801 ttataagaaa cccagactgt ttgatataag aattacagat gataaacatg tgatggctgt
    16861 tgatcttgca aagaatactt tctttaccaa ggatcaatta ataagtataa ttaaagaatc
    16921 gggcgctgag gaagtgaatg aaaaaaactt ttgagactac tactcatatg cgaattggaa
    16981 acaacatgaa caaaattatt gtagcaactt tcgcggttgt cttgttggga tgccgggccg
    17041 gtggtaatga cccgggattg gaatatgcac ccaacatgta tcatgctgtg ccttatgaac
    17101 cgcttgctca aattaaagac aaggatgcag gtaattgggt tagttctctt gaggatggta
    17161 aaggagaata ttacagttcc aatccttata acccttacga aatgaatatg cgaatgcctc
    17221 cggctaattc agtgccaaga acagcaggag gttaccttcc atacagaatt cccaaagata
    17281 gcctagaata tgcggcaaga accttaaaga gtccccttga ttcaacggct gccatcacgg
    17341 aagaagggaa attattatat gtacgattct gtcaacattg ccacggcgag agcggacaag
    17401 gtgatggacc cgtcggtgtg gcactggccg gagttcctgc atacaactcc gcgttggtga
    17461 aagataaacc ggaaggtcac attttccatg tcataacaat ggggaaaggt cggatgggtg
    17521 ctcatgcttc gatagtaagc cccgaagagc gatggaaaat cgtcaggtat gtagaacagc
    17581 ttcaaaagaa ataaccatga atttagacga acgatatact tttggttcct ccacaaaaaa
    17641 gaaactcctt gtatttgcag ctgtaggagt tgtggtgacc attatcggtt tgattatggt
    17701 tatgtccggt ggcggtgaac atggtgctga aaacgaacat gcagcaactt tagccgctaa
    17761 gaatctgttg gccgatgcaa acccatctgg tgcaacccct gttgccgatg caagcgaaga
    17821 atcacatggt tcggctccat ggctgaaaag acttttcgtt ggaatctgga tcaataatat
    17881 tttcttcatc ggaattgcca taatcggagt attctttgtg gcaatacaat acgcctcgca
    17941 ggcgggatgg tctgccggaa tgcagcgtat cccggaagca tttggatact ggctgccaat
    18001 tggagccatc attactttgg ttatgttttt tgttgccggt cacgatattt ttcattggac
    18061 acatacggat ttatatacag aaggtggcgc gaactatgat aaaataatca gcggtaaaag
    18121 agcgtatttc ttctggccat tacaagataa tccagggttt ccaattttct ttattgtacg
    18181 tatggtcgct tattttgcat tatggatctg gttctttatg atgttaagga aacacagtct
    18241 tgcggaggac cttgatgcca acacttctca ttggtacaaa atgagaagcc tttcagcaat
    18301 attcctcatt atatttgcag tcacttcttc caccgccgcc tgggactgga taatgtcaat
    18361 cgatacccat tggttctcca ctatgttcgg ctggtacaca tttgccagct ggtgggtatc
    18421 cggtcttgca atgacaactc ttattgtaat ttacctgaaa gagaaaggat tgctatctct
    18481 tgttaatgaa aatcaccttc acgatctggg taagttcgtt tgggcattta ccattttctg
    18541 gacctatatc tggtttggac aattcctact catttactat gcaaatattc cggaggaatc
    18601 aatttatttt catgagcgaa tgaagacaag taattactca gggatttttt attttaatct
    18661 gataataaac tttttcctcc catttcttat tcttatgaca agagatgcaa aaagaaagat
    18721 gacattcctg aaactcgtat gtacgatgct gcttattggg cattggttcg atttttattt
    18781 aatgataaca ccaggaagtt tgcaatttga tggaggtttc ggactccttg aaattggagt
    18841 gactatgata ttcctggcag gattcctata tgtagctctg gtaaatattg caaaggctcc
    18901 attaatagca aagaatcatc caatgctgga tgaaagtcgg catcatcata tttagaatat
    18961 gaagaaaaat tattttgaaa agtaatcaga tatgaaagta attgtaattg cggggattgt
    19021 actgattgtt gttatcctgt ttttgatttt cagaatagca actcttgttg gaattttgaa
    19081 aggtggtgag aaaagagatg gacaaagcaa tcagataaat gctttattgc ttttgatttt
    19141 tattatcgtt tcgggaagtg tattactttg gtatgcatat acaaaatatg atgaatacac
    19201 cttgcccgtc gcgtcggagc atggagttat cacagataac ctcttttggg taacaacact
    19261 gattactggt tttgtattca tcataaccca ggcacttcta ttttatttcc cgttcaaata
    19321 tcaatacaaa gaagggcgaa aagcattttt ctatccggat aacaacatga tagaaatgct
    19381 ctggacaatt atccctgcca tagcattggc aatcttgatt ttctatggtc ttactgtttg
    19441 gaatgatatc actgatgagg ctccacaaga ggcggaaaga atagaaattc ttggccatca
    19501 gtttgcctgg aaattgcgct attccggaag ggataacttg ctgggtaaac acgattaccg
    19561 cttgattatg gctgaaaatc cagttggagt agacttcact gacaagaatg catttgatga
    19621 ttttagtgcc gggcaaatgt atgtgccgaa aggaaggccg gttgaattaa gaattagagc
    19681 aagagatgtg cttcatagtg tttttgctcc tcattttcga ctgaagatgg acgcggtacc
    19741 cggaatggtg acatccttct ggtttgttcc aacaaagact actgcagaaa tgagagttga
    19801 aaccgggaat gttaatttta attatgaaat agcatgtact gaaatttgcg gttatgggca
    19861 tttcgggatg agaatgatag ttattgttgt tgaaccgaaa gaatttgacc aatggaaggc
    19921 cagccaggaa accctgattc aaagagagcc cggtctgctt aaatatgttt cagaagactt
    19981 aaaggaattg gcaagaataa aatcaggact tacggatatt caagataaag aaagtgaatc
    20041 ggtttcggtg gcgggatcga ataaatagat ttattatggc aacagcggaa atacatattg
    20101 aagaaggaca ttcagcggga ggtcacgagc atgaccatga tcatcaaggt aacttctgga
    20161 caaaatacat tttctcacaa gaccataaag taattgcaaa acaattcctc atctcaggaa
    20221 ttttctgggc tttattaggc ggcatattat cggtaatatt caggttgcaa ctgggttttc
    20281 ccgacatgaa tttaatgtgg ctaaaacctt ttcttggtgg ttggataacc gaagcaggaa
    20341 aacttgatca ggaattttac ctggcgctgg ttaccatgca cggtaccatt atggttttct
    20401 ttgtgctgac tgccggatta agcggaacct tcagcaattt tctgattccc ttacaagttg
    20461 gggcgaggga tatggccagt ggcttcctga acatgctatc ttattggttc tttttcctct
    20521 caagtgtggt catgtttagc tcccttttta tttctaccgg gccggcatcc ggaggttggg
    20581 taatatatcc gccattgagc gcccttcccc aggctatgtc aggatccgga ttaggaatga
    20641 ccctttggct ggtcgctatg gctttattta ttatttctac cctgttggga ggtattaact
    20701 atgtaaccac gattattaac ctcagaacgt atggaatgac atttaccaga cttccattaa
    20761 caatctgggc atttttcctt accgctgtta ttggaattct ttcattccct gtactttttg
    20821 ccgcggcatt attactgatt tttgacagaa gctttggtac aagcttttat ttgacagata
    20881 tatatataaa tggtgaggca ctccctaatt cgggaggaag tcctattcta ttccagcatt
    20941 tgttttggtt ccttggacat cctgaagtat acatcgtact attaccagcc ttggggatta
    21001 cctcagaaat aatttcaaca aattcaagga aacccatatt tggctacaga gcgatggtag
    21061 gctccctgct ggcaattaca tttttatcct tcgtagtctg ggcacaccat atgtttgtta
    21121 caggaatgaa tccttttctt ggatcaatat tcatgctgct aacattaatt attgcagtgc
    21181 catctgcagt aaaagtgttt aattacctcg ccaccctctg gaaaggaaat atcgtattta
    21241 cacccgcgat gttattttct atcggccttg tgtcttactt tatttccggc ggattaaccg
    21301 gaatctattt aggaaacgcg gccgtagata ttcaactgca tgatacttat tatgtcgtgg
    21361 cgcactttca tattgtgatg ggatctgcct cattcttcgg aatgctagcc ggtatttacc
    21421 attggtttcc taaaatgttt ggaagaatgt tagacaaccg gttgggatac atccatttct
    21481 ggttaacatt cgttggcgtt tacctggtct tcttcccaat gcattttatt ggaatggcag
    21541 gatttcccag gaggtattat tctttcacca actttgatgc cttccagtca tttaccgact
    21601 tgaatatgct gataaccatt gccgccataa ttactctggc agctcaattc atattcctgt
    21661 ttaatttctt ctacagcatt ttcaggggca gacctgcacc tgctaacccg tggagatcga
    21721 atacactgga gtggactact ccaattcatc cggggcatgg taactggccg ggtgaaattc
    21781 ctcacgttta ccgatggccg tatgattaca gcaagccggg tgcgaaggaa gactttattc
    21841 ctcagaatat tccattttca gatactatgg aatcgaatct gccggaagaa aatgaactta
    21901 ttaagctgga gggtgctgat agcggggcaa taaaggttga tgatacaaaa cactgataag
    21961 gagttagccg catttaagaa attaagcctg ataactttaa tagcggtgta ttttttgatt
    22021 cttgtcggag gaattgtgag aagcaccggt tccggaatgg gatgcccgga ttggcctaaa
    22081 tgttttggaa gctggattcc tccaacatca gagaatcaac ttccggaaaa ttatcaggac
    22141 aagtattcaa tgcaaagaca cgaaaagaat attaaactag ccaattatct ttcagccctg
    22201 gggatgaaaa acacggctat gcggatactg gatgatgaca acattagagt tgaggaagaa
    22261 tttaatgcag tgaaaacctg gacggagtat attaaccgtc ttgtgggggc aacaatcggc
    22321 ttcctgatct tcctgacaac actcgcttct tttcgccttt ggaagagaaa taaagttatt
    22381 gttattatgt cagtcattac cctgggcact ataggattcc aggggtggct gggcagcctg
    22441 gtggtttcta ctaaactgct tccgggtatg gttacaacac acatgttact cgccctggtt
    22501 attgttgcca tgattacctt tttgtggttt aaaggaaacg gcaataaatt tgaattaaaa
    22561 ggcggatcat taaacataat tattatacta agcctgattg cccttgcaat tcaggtttta
    22621 ttgggaaccc aggtgagaga aagggttgat gtggcgggtg ccgctgctgt aaattcaggg
    22681 gattgggtaa atcaaattgg tctgattttt tatgtgcaca gatctttttc aattattata
    22741 ttaataataa gcattctcct ggtgaacaaa attgtgaaag agattggtag agaaaatcaa
    22801 cattacaaac tttccagaat tgttgccggc ctggtaatac tcgaaatgat gacaggagct
    22861 tcaatggcat atttttcaat cccacctttt ttgcaaccca tacatctttt gcttggaacc
    22921 ctggtgtttg gattaacttt tcttttgttc ctgagtatgt catctgaaaa atatcaaatt
    22981 gaagttgtga aatgaatagc cccggaacaa atatcatcat taaaacaggt gtcctttata
    23041 ggacgagagc cttcgtagaa ttactaaaga cgcggctttc attcctcgtt gctttttctt
    23101 ctggtttcgg atatatactg gctacaaact cattcaactg gattcaattt acgtgtttgt
    23161 tcttaggagg attcctggtt agcggaggtg ctgtaactat aaatcaaatc ctggagaaag
    23221 atgttgatgc cattatgaac agaacgaaag gccggcccat tccaacagga agggtttcaa
    23281 taccagaggc caccattttt tccatcctga tcagttttgc aggcttaatg ctcctgcttt
    23341 attcaacgaa tatccttacc gcaactttat cattagtttc tctcattctc tacagctttg
    23401 tttatacacc ccttaaacgc gtaggttcca tcgcggtatt tgttggtgca attcccggtg
    23461 cgctgccacc acttcttggg tgggtcgccg ccaccggcca gatttcggta gaagctatta
    23521 tgctttttag tattcagttt gtctggcagt ttcctcactt ttgggctatt gcctgggttt
    23581 cctatgatga ttacaaaaaa gccggcatta aattattgcc ttctggcgga gggaagaatc
    23641 tcaatacggc tattcaaata atgacttaca cattgttcct tttgcctttg ggaattttgc
    23701 ccgctaagct cggtatcaca ggagtaaatt ccgctttggt agccacaatt tgtggagctt
    23761 tatttcttgc tcaaacattt tcattaatga aggatggctc aagaaaatca gctttaagaa
    23821 taatgtatgg ttcgttcctt tatttaccta ttgttcaaat tgcgtatttg ttagacaaga
    23881 tataatatgg tgaaggatat acaattaacg gaagaaccaa ggaaaacctt tacgatggat
    23941 cccaaaaaat tcattctgtg gctttttatt gttagtatta ttatgttatt cggagcattt
    24001 accagtgctt atatggtaaa aaaagcatcg ggagattggc taagtatagc gttgccagga
    24061 atattctact ttaatacagt agtaatttta ctaagcagtg cgagtatgca cattgcggta
    24121 atgtcaacaa aaaagaataa tctcaaattg acgcggatta cacttgtaat cacttccatt
    24181 ttggggctta cttttcttgc aggacaatat tcaggttggc aagatctggt gagaaataat
    24241 gtcttctttg ttggccatcc atccggttca ttcatttacg tgttaaccgg agttcatgga
    24301 atacatttgg tgagtggagt gatttttcta ttcatcatag tggctgccgc atttagattt
    24361 aaagttcata gcaagagcca gagatggatt caaatgtgtg caacttattg gcattttttg
    24421 gatgcccttt ggctatattt gtttatcttt ttattattga ataattaact caaacaagca
    24481 aggatatgtc ttcaaccacc gctgcagtat ctgacggaaa gaagctctgg tacggaggtc
    24541 aatcgcccgt gaaggcgagt tatggaaaat taatgatgtg gattttcctc gtttcagatg
    24601 cattcacatt ctcagcactt ctgataactt acggcctcat ccgctattct catcctgctt
    24661 ttgaaggtgc ggtaagggaa tttactttct ccactgaata ctggccggtt ccggaaaaag
    24721 tttttgctgc aattccattc tttcatggac taaatgctcc tctggtattt gttggtttaa
    24781 tgacattcat tttgatcctc agcagcgtaa ctatggtgct cgcggtggag gcaggccatc
    24841 gaatggatcg gaaaaacgtc gaaaaatgga tgctttggac tattgtcggt ggtcttgcat
    24901 tccttggatg ccaggcctgg gagtggactc attttattat cggcacaggt gaagcaacca
    24961 tggcaaagag atttgcggat ggtgcctggc aatcctttga agtcttggga gcaaatctta
    25021 gcattaatca atatggaccg ccagattttg cggcgttatt cttttttata accggatttc
    25081 atggcactca cgtttttagc ggtgtgctgc tcaatttcct gatattccat aatgccacga
    25141 caggtgtata tgacagaaga ggtcactatg aaatggttga gaaagtggga ttgtattggc
    25201 actttgtaga cctggtttgg gtgttcgtat ttacgttttt ctatcttgta taataagttg
    25261 aaatattata atcatggaag aaactaaaga aattatagtt caacctgtaa ataaggaaaa
    25321 agtccggcaa ttggtaagag ttgccatcat tttgggtatt gtcaccggcc ttgagtttct
    25381 ggtagcattt acgtttcact caggtcctct gaaaacctca attttcgttg ctatgaccat
    25441 aataaaagct ttctatatag ttggcgaatt tatgcacctg cgtcatgaag tgaagatgtt
    25501 gatttggtcg atcttgctgc cgatgatgtt tgtggtgtgg ttactgatta ctcttatcta
    25561 tgagggtgga tcaattcttc aggtcagata gatacttaat aaaaatctca acccaatcag
    25621 gcaagagcct gatttttttt taacctgaac cgcttgtgaa aaagtttgtg cttcttgtag
    25681 tagtaatcat cccggtgggt ctcgtattgt tcgtgaaatt cttcggaaag aatgaatata
    25741 atctccctgt ttattaccag aatggatatc ccgcgataac tgattgtact cttgttggtg
    25801 cggcagtttt ggaacctgca ggattggcct ttgaaaacct taaccgggtt aatttgattt
    25861 tctatgaacc agagtgtgaa tcgaacctgg actttttcaa tagtcaaact gagagaatca
    25921 gattaaatat gagtgaggct gactttatta attacattac aatttcttca gtcgattcaa
    25981 catattcaca ggaagtgaga aaaaaatttg ccgtatgtgg attgttaatt gctgaacaaa
    26041 aagaggaaaa tctgaaggat tggaaatgca ctggatttat tgtaatgatt gattggaagg
    26101 ccagaatcag aggatattat tcgggaactg attttgagga tattgaccgg gtaatcctgg
    26161 aattgaatgt tatggaaact atagttaata atgaatagta aaaaccggcc gatttggatc
    26221 ataatttcaa cttccatagt catacctgta ctggtagcga tcctgttttt ttcagaagga
    26281 atcatcccct tgttgggtga ttgggtctat tcacttcccc aggttaatgc agtcataaat
    26341 tcggttaccg ccatcttact gatcgctgga aagtatcaaa taagaatggg caataaagag
    26401 ttgcataagc gactaatgat tacttccttc atcctgggca tagtgtttct actctgctat
    26461 gttacttatc acgcttcggt tccttctacg cattaccaag gagaaggatt tctgaaatac
    26521 atttactttt ttctattggt aacccatatt attacagcaa taattgttgt tccatttgtt
    26581 ttgctggctc tattttacgc tcttcaggag aatttcttca gacacaggaa aattgttaaa
    26641 tatgcccatc ccttatggtt gtatgttagc attacaggag ttattgttta tcttatgatt
    26701 agcccttact attaagaatt ttaataaatt tggagtatga agcgagtctt gttgcttgtc
    26761 attgctctga tcgtatctta tggaacgagt tttgcgcaat gtgccatgtg cagggcaact
    26821 ttggaaacca gcgtgagcaa tggggaatct ctggagatgg cttcaacctt gaattttgcc
    26881 atcctttatt tgcttgcact gccttactta ttagccctgg ttatagggct tctttggtac
    26941 agaagggcca ggggcaaggg tactgcttcc taagcagagt tgtgcccgct atttcaatat
    27001 tttgtatttt tagtgaaaaa tgaaccggag tctgctcttc ttggtgggtt tggtattaat
    27061 cctttcgggg attctgatta aatatttcct tcttccgaag gaagctgaac cacaggaaat
    27121 agcaaatagg attgaaaaaa atctccaaac cgaaatctca atcagcaaat cacaactcgc
    27181 ggtagtaagg gaaaggataa tgaatgatga ttatcttcga tttgaagact tgaatacaat
    27241 tgcatctgat tacccatttt atgtctatta ccgaaccagg ctattctact ggtctgagtt
    27301 tcaactcgtg cctgaataca atgaattttc agatgttgga acaattgggt ttatccatct
    27361 cgaccaggga aattttatag taaccaggga tcagtttaat aagaaagatg acatctatga
    27421 aatagtttcc ctcattcccg ttcaaacaga atacgaaatt tttaataaat atctggaatc
    27481 agattacaat gaccggataa ttgagtttcc tgatatgata atttccaatt cgggaaatga
    27541 ggggtatccc gtaaaattcg acggtcaaat attattttat gtcgacagct atataagcgt
    27601 aaaccagtac tccatccact tccagttgat ttcgatttct ttatacacaa tagggttaat
    27661 attaatatta tacacggttt actccaaagt attggagtta acaaatcgaa ggaaatttga
    27721 aatcgctctt gcaattttat ttataagtct ggctggggtg agattcgtca tgctttttac
    27781 agaatttcca tcagcatacg ccaattgggt gctctttgat tcgaggttat ttgcatcctc
    27841 tattttgaat ccatcaatag gtgacctgat gcttaacaca cttgcactgt ttgcactgat
    27901 cctttttgcc tacatgaatt attatcggag caacttattt ataaaaattc taggtctaaa
    27961 aataattgca aggtggatcc tttctgttaa gcttattctg attggctttt tagcaatggt
    28021 atatcaattc ctgattttca gattgattta tgaaaattct cagtggtctt tagatatcac
    28081 atcaaccttg tattttcccg cttccaaggt tattttttac ctgatatttg taatcaattc
    28141 tgccatattt tttctaacat ttcatttgat ctttagaata ttcaccaggg taaatgacaa
    28201 atcattatac agtctcatca taaactattc cgttggggca ctgattttta ttttagttgc
    28261 ctacaatctc aatttgccta ttgttttcat tatcatggta aatctgggat attttctggt
    28321 tttacatttt ttgaaattat ccagattcct ggttcaaatg agatatctgg cttttcttta
    28381 tttattcgtt gccgcccagg catgtgcact tataggagct tattcagttc aatattttga
    28441 agaagaaaat gagttggaaa ataaattcag attcgcggac caattccttg ttgaaagtga
    28501 caatttgggt gaattactgc tatctgaagc tggatttcaa ataaagcagg atccgctgat
    28561 aaccagcagg atgtatagtt cctttatgtc caaagaaaca atttctcaga aaattaaaag
    28621 ggtttacctt ggaagttact ttgataaata taatgtacag gtaaacttat acaatgcgac
    28681 cggtcagcca tttaattcgg agggaactca ggggacctac agacaaataa aagatgcatt
    28741 tgcccaggaa caatatgaaa cggagtttcc aaatttgtat ttcgtaaata gagtcggtgc
    28801 ggatgttacc aaaaggtatt tatatttcat tgatatagaa aggtacaatc aggttatagg
    28861 ttatatcctt attgatcttt cactgaaaaa aataataccc gaatcagtat atccggaatt
    28921 attggttgac agcagattct ttcagcctta ccaggaatcg gagtacagtt atgcagtatt
    28981 tattgggggc cgggcaacct attcaacagg aaattataat tatacagttg ggtttgataa
    29041 tgatctttta gagaaccctt cactttttaa ccgcgggtta tcttatagag gtcaccacca
    29101 ttttgccgtg aaagatctgg acagcagaac gattgttatt acttctactt tgtaccctgc
    29161 aattaatgta gtttcaaact tttcttttct ctttttgatt ctggtggcaa ttattatttt
    29221 aattatagtt ttttacagca tttacaactg gcttaatgat gttaagctga aattcgcgac
    29281 taagattcaa ctttatatta acctggcctt tattattcca ctgtttgttg taagcatagc
    29341 tacattaagc ctgattaata aatcttttag aaaggagctt gaaattgaat actataagaa
    29401 agcagaaaat cttggccgga atattattgg tgatttgaat gaatatttga agcttgacct
    29461 tgatcgtgag gatttgggaa acaggttggc aagcataacg gcaaatgcaa atcttgatgc
    29521 aaacctttat aacatccgcg gtaagctaat cggatcaagc caaccttcca tattcgataa
    29581 gaagatcatc agtgaatata taaatccaaa agtaatagag ctaataaggg aagatggcaa
    29641 cagcgaagca atcctgagag aacgtatagg tagtcttacc tacaatgccg cttatgttgc
    29701 attgaagtct tttaatgatg gcagtgttct tggaattttg aatattccat tcttcgattc
    29761 tgagaccatt gttgagcgcc aacagattga cgttctttca aacataatga atattttcac
    29821 cgctattttc ctcatacttt tgtttttgtc ttatttagcc agcaaatggc taaccttacc
    29881 attacaaatg attacccaaa ggattaggaa aatttcgttg tctggtgaaa ataaaccatt
    29941 ggcatggaat tctgaggatg aaatagggtt acttgttggc gaatataaca ggatgttgat
    30001 aaatcttgaa gtgagcaaaa aagccctggc aaagagtgaa aaagagtcag catggcgcga
    30061 aatggcgcag caggttgcac atgaaataaa aaaccccctt actcccatga aactgacatt
    30121 gcagcaattg gaaaaatctc ttgaggaatt gaaattgcaa aaaggatcaa ctgagaaacc
    30181 gataaaaact cttttgtacc aaattgataa cctgagtgat atagcgtcat ccttcagcgc
    30241 ctttgctaaa atgccgattc cggaaatgaa gaacattgag ctcacctctt tgctgagaag
    30301 cacttttgat ctatataaga atcaggacaa ggggaaagtg acgttgaatc aataccaccg
    30361 tgaactttat gtattaggtg acgatcaatt aatgggtcgg atcttttcca acatgatcat
    30421 taattccttg caatcttcaa acgatgacga cttggtaacc gtagttggaa ctatccatgt
    30481 aaatgggaaa gggaatgtac ttattacaat taaagatgac ggcaatggca tacctatgga
    30541 gatcagggat aagatctttg tccctaattt cagcacaaaa actaatggct ccggcattgg
    30601 cctggcaata gctaagcacg gaattgaaaa tatgggtggt aaaatctggt ttgaaagcga
    30661 aattggaatt ggaacaacat tttttattga gatcccactt gattccaaag gatcatttct
    30721 tgtgggctcg cattagctta ttggggaata aaaattcatt gagttccgga atatcggttt
    30781 tatctatatc ctgattactt ccttcccaca gctttgctcc ttcgtgcagg aacataacat
    30841 attcgccaat ttccataacc gagttcatat cgtgagaaac caccacagtg gtaatactat
    30901 attcttgggt tatttcgtga atcaggtcat ctatttttat cgcggtgagc gggtcaagtc
    30961 cggaattagg ctcatcacaa aacaaatatt ttgaattgtt aacaatggcc ctggcaatac
    31021 ccactctttt tttcattccg ccagaaattt ccgagggcat tttcttattt atattttcga
    31081 gccccactct gcttagaaca aaatttaccc ggtcaatttt ttcatgctcc ggcatttttg
    31141 tcaaaatatc aagaggaaat tttacattct gctccacatt cttcgaatcg aataatgcac
    31201 cgccctgaaa caacatgcca atttctctcc ggatttctgt cttcaggtta cgatgagcat
    31261 tagtaaattc gcgttcatta taaaaaacct cccctatatc aggctttaaa agaccaacaa
    31321 tacatttgag aagtacactc ttaccagttc cactggctcc aattatcatg ctagtggttc
    31381 cgcgcttgaa aattccatcg attcctgtaa gaacagtttt cccttcaaaa ctcttatgaa
    31441 tattccggat ttctatcata gtaataactg cgccagtaaa taatctgcaa ccagaatagc
    31501 gattacacta tttgtaaccg cggaggtgct tgcttgtcct acttccaatg ctcctccctc
    31561 ggtataaaaa cctttaaacg aagagatact ggaaatcaga aatgcaaacg tgtacgattt
    31621 tattaaagca aatgttatgg tgtattctac gaacgatgca cgaataccaa aaatgtattc
    31681 ttccggagta acaacaccac ttaatgttcc ggcaatgtag cctccccaga tgcttaatgt
    31741 tcccgccaga actaccagca agggatacat gatcaaggtg gcaagaattt ttggcagaac
    31801 caggtaggaa accgaattaa ttcccataac ctcaagggca tcaatctgtt cagtaattct
    31861 catcgtaccc aggcctccgg ctatgctcga gccgactttt cccgcgaaca cgactgctgt
    31921 tatagtagga gcaagttcca gcactgtcat atcccgaacc accattgata tcacatattt
    31981 ggctatcagg gggcttacga gattgtaggc cgtttggata gctgttacag cacctatgaa
    32041 caatgagacc agtgaaacaa gtacgataga attgatgcca attaaaattg cctcatcaaa
    32101 tacaagtttc aaatatgttc tgaacgattc cctgtttatt aacagagaac ccaggaagat
    32161 aaagtatttt ccaattgcac ttaataagcc catagcgccc taaaaggtat tttcctgatg
    32221 attaactacc tttaccgcca agaaagcgga taatcccaaa atttacaaat attattgata
    32281 cctcagatgc aaaagacagc tattataaca ggcggcacca aaggcattgg aaaatcactc
    32341 gttgaggaat ttgttc
//
LOCUS       DASWHX010000217         2627 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_3558336, whole genome shotgun sequence.
ACCESSION   DASWHX010000217 DASWHX010000000
VERSION     DASWHX010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 2627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2627
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3558336"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(265..>2627)
                     /locus_tag="VGA21_14290"
     CDS             complement(265..>2627)
                     /locus_tag="VGA21_14290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018479581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD
                     domain-containing protein"
                     /protein_id="HEX9651729.1"
                     /translation="ADPNPGDEIELVFKATIDPKWYLYSSDFDPEVGPMVTTFEFESS
                     DDYELLGGINLVDPKKKYDSIFEGEYTYFKNNAEFRQKIKLNGNSTVLKGTYSYQVCS
                     DIDGKCIPFDDDFEIPVLLSGISTQVTPFGQPANPLGQQLLRPATWEFGLSNPDYSDG
                     DEIELIFTAHIDENWYLYSSDFDPEVGPMVTTFSFEKNDTYELTGDINPVGAMKKYDS
                     IFEGEYTYFKKKGEFRQRVKILEANTFFVSGTVEYQVCSDIDGKCIPFEDEFIFGNEP
                     AGKETPSPKKTSLGLTERDSTSPYSLLAFLGFAFIAGLAALLTPCVFPMIPVTVTYFT
                     KTAESSGGKSMALLYGLSIVVIYTIIGVGVSFLFGAEVANELATSAAANLIFFAVFVL
                     FGLSFLGLFEINLPSSWLTKVDSQADRGGIIGIIFMALTLCLVSFSCTGPIVGSILVE
                     SAGGAFLKPIIGMFGFSMAFAIPFTLFAFFPGALSKIPKSGGWLNSVKVVLGFIELAL
                     SLKFLSIVDQVYHWGILDREIFLAIWIIILIMMGFYLLGKIRLKGDDEAQSISVTRLL
                     FAIATFSFVAYLIPGMFGAPLKALAGYLPPMTTHDFDVPAMIRSYQSGIAGGNEQELC
                     ENPLHADILEFPHGIKGYFDYDQAISCARKQNKPLFIDFTGHGCVNCREMEARVWSDP
                     EVLKRLRNDFVLVALYVDDKKELPESEWYTSSYDGKIKKTIGRQNADFQISRFRNNAQ
                     PFYVILDNEENLLIEPKAYDLNVQNFIKFLEEGKKNFYSKNQIVRK"
BASE COUNT          815 a          562 c          478 g          772 t
ORIGIN      
        1 tcagcacaaa aatgattttt aaaaccttca ttttatctct gtgaaatcat ttaaaaatcc
       61 aaaaaatcat tcaatgatac ttaaatttta ctaattctga aataaaaccg gatttcgggt
      121 agcgtctaag tttggaatca ttatacgtgt gccacacttt ttcgatatgg aaaagcaatc
      181 aatttcacct aataaagacg gaaatgtggc acaggtagat attaattttg cagattattt
      241 atagtgtgca ttgtaagggt agttttactt tctgacaatt tggtttttag aataaaaatt
      301 ctttttcccc tcttccagaa atttaataaa attctgaaca ttcaaatcgt atgcttttgg
      361 ttcaattaat aaattttcct cattatctaa aataacgtag aacggttgag cattatttcg
      421 gaaccgcgaa atctgaaaat ctgcattttg ccttccaatg gttttcttta ttttcccatc
      481 ataagatgag gtgtaccatt cgctttccgg taactctttt ttgtcatcta catataaggc
      541 taccagtaca aaatcattcc tgagtcgttt caaaacttct ggatcagacc acactcttgc
      601 ttccatttcc cggcagttca cacaaccgtg accggtaaaa tctataaaca atggcttatt
      661 ttgttttctt gcgcaagaaa ttgcctgatc gtaatcaaaa tatcccttta tcccatgtgg
      721 aaattccagg atgtcggcat gcagaggatt ttcgcaaagt tcctgttcgt tgccgcctgc
      781 tattcctgac tggtaacttc taatcatggc gggtacatcg aagtcatggg ttgtcatggg
      841 tggtaaatac cctgcaagcg cttttaatgg agcaccgaac atccctggaa tcaggtatgc
      901 tacaaaagaa aatgtagcaa tggcaaacaa cagccgggtt acactgattg attgtgcctc
      961 atcatcacct ttaagtctga ttttccccaa caggtagaat cccatcatga taagaattat
     1021 tatccagatg gcgagaaaaa tctctctgtc caaaatcccc caatgataga cctgatcaac
     1081 aatggataga aatttcaatg ataaagcaag ctcaataaag cccagaacaa cttttacaga
     1141 attcaaccac cctccggatt tcggaatctt tgaaagagct cccggaaaaa atgcaaagag
     1201 agtaaaggga attgcaaacg ccatgctgaa tccaaacatg ccaattattg gctttagaaa
     1261 agcaccaccg gctgactcta ccaggatgct gccaacaata ggtccggtac acgaaaagga
     1321 taccaggcac aatgtgagcg ccatgaatat tatgcctata atacctcccc tgtctgcctg
     1381 actatctact ttggtcagcc aactgcttgg taaattaatt tcaaacaacc cgagaaagga
     1441 caatccgaaa agtacaaata cggcaaaaaa gataaggttt gcggcagcac tggtagcaag
     1501 ttcgttggca acttcggctc caaaaagaaa cgaaacaccc acaccaataa tggtgtaaat
     1561 aaccacaatg ctcaacccgt acaataatgc catacttttg cctcccgatg attcagctgt
     1621 ttttgtaaag taggttaccg taactggaat cattggaaaa acgcaaggcg ttaagagggc
     1681 agcaagtcct gctataaatg caaaacctaa aaaggctaat aatgaatatg gactggtgct
     1741 gtctcgttct gtcaggccta aagaggtctt cttaggagaa ggagtttcct tcccggctgg
     1801 ttcatttccg aaaataaatt catcttcaaa tggaatgcat tttccatcta tatcggaaca
     1861 cacctgatac tccaccgtcc ctgacacaaa aaaagtatta gcctccagta tttttacccg
     1921 ctgtctgaat tcgcctttct ttttgaaata tgtgtactct ccttcaaaaa tgctgtcata
     1981 ctttttcatt gctcctaccg gatttatatc tcctgtcaat tcataggtgt cattcttttc
     2041 aaaggaaaaa gtagtgacca tggggccaac ttcaggatca aaatctgaag agtagagata
     2101 ccaattctca tcaatgtgcg ctgtaaatat caattcgatc tcatcaccat ctgaataatc
     2161 cggattggat aaaccaaatt cccatgttgc aggtcttaac aattgctggc caagaggatt
     2221 tgcgggctgt ccaaaaggag taacctgagt ggaaatgccg gacagcagaa ctggaatttc
     2281 gaaatcatca tcaaaaggaa tacactttcc atctatgtcc gagcaaacct gatacgaata
     2341 tgttcctttt aataccgtgg aattcccgtt taatttaatt ttttgcctga actccgcgtt
     2401 gtttttgaaa taggtatatt ctccttcaaa aatgctatca tactttttct ttggatcgac
     2461 gagatttatg cctccaagta attcataatc gtcagaagat tcgaactcga aggtggtaac
     2521 cattgggccg acttccggat caaaatcgct ggagtataaa taccatttgg gatctattgt
     2581 agctttaaag accagttcga tttcatcgcc agggtttggg tccgccg
//
LOCUS       DASWHX010000218        25060 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_1750424, whole genome shotgun sequence.
ACCESSION   DASWHX010000218 DASWHX010000000
VERSION     DASWHX010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 25060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25060
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1750424"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(507..2750)
                     /locus_tag="VGA21_14295"
     CDS             complement(507..2750)
                     /locus_tag="VGA21_14295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018473267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HEX9651730.1"
                     /translation="MFNVRLHVIIYSILFFGCADRSRPIADANPYQDKVDSILNLMTL
                     EEKIGQLTLFTSHWSVTGPTLGEENRKYIVEGKAGNIFNAHTAGYNRELQRLAVEESR
                     MKIPLLLGSDVIHGHKTIFPIPLAEACTWDLDIIQKAARYSAMEATASGLNWNFNPMV
                     DIARDPRWGRISEGSGEDTYLGSLIAAAKVRGYQGDDLSDHSTMLACVKHFAAYGAAQ
                     AGRDYHTVDLSERTLREFYLPLFKAAIDAGSGSVMASFNELNGIPATGNHFLLTEILR
                     NEWRFQGFVVSDYTGINEMIPHGFAFDLKNAGELAVNAGIDMDMQGSVFLNHLSESVK
                     EGKVSETTIDASVRRILTAKFQLGLFDDPYRYTDTLREKEVIYSEEILSHARETARQS
                     IVLLKNEEFEGRKILPLSKSIKRIAIIGPLGDNKEDQLGTWHAAGDASKVVSLLEGVR
                     EATPDAEVNVVHGCGFTGEDRSGFSEAVKIAKQSEVVILAVGENYQQSGEAASRSELD
                     LPGLQQQLVEEIANTGKPVVVIVMAGRPLTIAWIAENMPAVLNAWHLGTMAGHAIADV
                     LFGDHNPSGKLTITFPRNVGQIPIFYNMKNTGRPFDAGNKYTSKYLDVLNEPLYPFGY
                     GLSYTQFSYSEIELSKSTITLNDSLDVSVNITNSGDVTGSEIAQLYIRDLVGSVTRPV
                     KELKGFRKITLQPRESRNIHFTITIDDLSFYRQDMSYGAEPGKFKVFVGTSSAETKEA
                     EFELRSH"
     gene            complement(2764..4284)
                     /locus_tag="VGA21_14300"
     CDS             complement(2764..4284)
                     /locus_tag="VGA21_14300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009582089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HEX9651731.1"
                     /translation="MNKNIVSFIIIIILIQACSGNTHDEFVLEKANNNKPLNIVFILS
                     DDHRYDFMGFTGKVPFLQTPNMDKMALQGAHIQNAFVTTSLCSPSRASILTGQYAHHH
                     GVVDNQSPIADSVNFFPQYLQRAGYETAFVGKWHMGHDNDEPRKGFDRWVSFRGQGTY
                     YNPAMNVDGERIEYKDSTYITDLLTDYAVDWLKTRTSEQPFFMYLSHKAVHSEFHPPK
                     RHNGKYQGKPIQYPLTMFPPGSDDPRAEGINNDSIYNYKDVPDWVKKQRYSWHGVDYM
                     YHGNINFENFYYRYNETLLAVDESIGRIIEYLEEAGLMESTIIFYMGDNGFAFGEHGL
                     IDKRQAYEESMRVPLLAFSPANIKPGTIIKPVIQNIDIAPTILELAGLKMPSNMDGMP
                     FSRMLLGEEIPWRDKLFYEYFWERPFPQTPTVFAVRSDQYKFIRYQGIWDINEFFDLQ
                     NDPLESNNLIRNEEYQDIIADMNQELFDWLKKTNGMQIPLRRDQGKRFDNKYSGTY"
     gene            complement(4322..5698)
                     /locus_tag="VGA21_14305"
     CDS             complement(4322..5698)
                     /locus_tag="VGA21_14305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009582085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HEX9651732.1"
                     /translation="MKIKLDSFCFGVSVFFVVFVSCNSGGKSSTSEDINNSRAISDDS
                     LMTLIQYRTFQYFWDGAEPVSGLARERIHMDGIYPQNDQTVITSGGSGFGIMAILVGI
                     ERGFITRQQGFERLNHIADYLKNAERFHGAWSHWLYGETGKAKPFSPKDNGGDLVETA
                     YLVQGLIAARQYFREGNDSEMELAAKLDTLWKEVEWDWYRKNDENVLYWHWSPQYQWE
                     MNFPVKGYNECLIMYVMAAASPTHGIPAEVYHEGWARSGEIRSANEKYGYKLILSHNG
                     AEEYGGPLFWAHYSYLGIDPRGLKDQYADYWELNRNHTLINRQYCIENQMNFEGYGEN
                     CWGLTSSYSPKGYAGHSPGRDLGVISPTAALSSFPYTPEYSMQAMRFFYEELGDKLLG
                     EYGFYDAFSIQDDWFPQRYLAIDQGPIVVMIENHRTGLIWDLLMSSEEVKQGLAKLGF
                     TYNEKHRY"
     gene            complement(5717..7705)
                     /locus_tag="VGA21_14310"
     CDS             complement(5717..7705)
                     /locus_tag="VGA21_14310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009195261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HEX9651733.1"
                     /translation="MKRGISYILRPSLLILLLCSCKKDMEISPPTIQLNSIRVGSYEL
                     DLDDQQLNSETPYDQPIIASFSASLDTTSLKNSVTLQSVSGESYSLNFSFQDNYKTFS
                     ATPSTNLSSMDNFQLEIANTLRGNDGEVFPGVVVDFSTKPGTMNITSLLMSEKEVINN
                     SRITDIPLVGTIEIMFSKPVDPDYMSGDYFKILGIQNIPLSILLSEDKKKVTLTISQP
                     LRHLTRYRLWISGDITGLNGESFTQMNRYFYSAINTEPVLPVISDEDLLTLVQQQTFK
                     YFWDFAEPVSGMARERNISGSLVTTGGSGFGVMAIIVAIERGFITRQQGIDRLSQILN
                     FLETADRFHGVWSHWLDGTTGSVIPFGTEDDGGDLVETSFMIQGLLTFRQYLNSNVLQ
                     ENDLITRINMLWEEVEWDWFTQGGQNVLYWHWSPNHGWNMNHQIRGHNETLITYFLAA
                     ASPTHAIDASAYHLGYAINGDIVNGNSYYNIILPLGQAYGGPLFFSHYSFLGLDPRNL
                     RDTYCSDYFLQNANHTLINQAYCLDNPKDFVGYSDLNWGLTASDNQIGYSAHSPTNDL
                     GVITPTAALSSFPYAPEQSMKALKFFYYNLGDRLWGPYGFYDAYNITEGWTATSTIAI
                     DQGPIIIMIENYRTGLLWDLFMSAPEVQTAMNKLDFTN"
     gene            complement(7777..9033)
                     /locus_tag="VGA21_14315"
     CDS             complement(7777..9033)
                     /locus_tag="VGA21_14315"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG-like jellyroll fold domain-containing
                     protein"
                     /protein_id="HEX9651734.1"
                     /translation="MKLKRVNWILSIATIFGMTTALLMSCKDDEPVELALVSLTADGI
                     DLNAATSPTNVPPGATITAEFNTSINESTLAVILTRDYDNTTMNLNVSVVGSTVTIDP
                     VDDFGTGTLFVLAIAPGLTSAEGKVLTQGIERNFTTDGAFAPAGAVAHWTFEDNANDV
                     IGAFDPTTSQVVDITYVASRNTTAGKAASFNGSTSIIEISNGDDLMNDGSWTLSFWVK
                     PSSANGKTSGHFVLGLGAFYGFQFEIAGDYSNCKLAARYGYNHPTNGPGTASEDLWVD
                     ATGNLGWQGWTFSKDLSGSGGLEAVIKDQWTHVALVYNAVEKTGTAYINGEKMKEQDF
                     DLWPEGDNKTYVSGLEYDGVAPEVVNELAFGFIQSRAGTLWDNETWGGYDFASANHFQ
                     GELDDIRIYFKTLTLTEIDLMYNSEK"
     gene            complement(9109..10608)
                     /locus_tag="VGA21_14320"
     CDS             complement(9109..10608)
                     /locus_tag="VGA21_14320"
                     /inference="COORDINATES: protein motif:HMM:NF019592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HEX9651735.1"
                     /translation="MKIYISIVRVIKKGGLISLLILSSCEDFLEKPPQGMLTQETFPT
                     TANDAELAVNAVYNTLRIPNFNSGLFPILDIMSDDANKGSNPDDAANAIGPYDRFEHV
                     ATEGSLLRWWNTLYEGIKRANVVIEIVPDIAMDLTLQNRNIAEASFLRALFYFDMVRA
                     WGGVPIITSTEPSADATRATKAEVFSLIEDDLLFAIANLPEKSGYGNPADLGRATKGA
                     AKSLLARVYLFQQNFADAASLTLEVINSGEYNLEMNFEDANSVLGEHGIESVFEVGAF
                     GQEGIQNGGNQYANVQGVRGTPNRGWGFNRPTMDLMNAFEPNDPRMDATIIFLGEVLD
                     GVPIMGDGSTPDETTDSHGNIELETYNQKVWTPGSNVPPSHGHNRRIIRYADVLLMAA
                     EALNEVGNPGQALVYLNLVRERARQGNNTILPDITETDQTLLRDIIYNERRVELALEG
                     QRFWDLLRTGQAQSVLGPLGFTINKDELLPLPQDEIDLTQGRLEQNPGW"
     gene            complement(10623..13619)
                     /locus_tag="VGA21_14325"
     CDS             complement(10623..13619)
                     /locus_tag="VGA21_14325"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX9651736.1"
                     /translation="MFRFMTPLNRVLTFSLAIILFFPEQTQAQRVIVRGQVKEAGTGE
                     TLPGVNVVIKGTNIGTVTDIDGRYTIEISEPTNSMLIFSYVGYVTQEMAVNNQTTIDI
                     TLEEDIDILQEIVVIGYGTVRKSDLTGSVSSIRGKDLGKVTSSNPMQSLQGKVPGVQV
                     SSASGAPGSSVIVRIRGQGTFNVAAPIYVVDGVIVQDIDFLNSADIESVDILKDASAT
                     AIYGSRGANGVVMITTKKGKLGEPVISFNAEYSIQKLEKKIDLLNGREFAIVANQIKS
                     GSYNNVDLVPDTDWQDLIFDIAPMQHYQISASGASEKTSYYIGMGYFNQEGIITKSNY
                     ERVTLKLNNSYNITNFIKIGNNIAVTPYKRQNTNGNAVFVAYRAWPTLEPFQPDGTYT
                     PVPGVGNVLADIEYTNSFDNGLRTVGNFYGDVDFLKGIKFRSSFGVDIDNVKSKRFTP
                     IYFVNSQQENSTDDLTKNYFFKTDWLWENTLSFNNETGPHYLDAVIGFTMQESSSEQV
                     TLVGENLLRPDQDFWYINPNNINPNGILNGVNDDLNFSMISYLARFNYTLKDKYLFTA
                     TFRRDGSSKFTKENKYANFPSFAVGWNLINENFLREIPFLSNLKLRASWGIVGNEKIR
                     YSSQFSLISNGLNAVFGTEDVQYPGSSYGISGNSGIKWENTHQTDIGFEFGFFGDQLI
                     GELDYYNKRTKDILIPLLVPGYFGNGDGATIFFNAGEVVNKGLEYILAFRGETSSINY
                     RIGTNGTTIHNETVKVRGTGGSDDELGGFANGQVTRSAPGLPIGAFYGYIVDGIFQNS
                     AELNSYPHLNNTGVGDLRYRDVNADGVLNGDDRTMIGSPIPDFVYGLNLEVGYKGVEL
                     SIDFQGQAGNEIYNNKETVRPDLYNFEQRYFNYWTGDGSTNTDPRPSSGGVNFNNPSS
                     HFIYNGSFFRLRSLTLAYNFPTSLISRARIQSAKVYFSGTNVFTISDYTGYSPEVSSE
                     IVNLNGIDFGGYPVSQIFSIGLNVNF"
     gene            complement(13932..15788)
                     /locus_tag="VGA21_14330"
     CDS             complement(13932..15788)
                     /locus_tag="VGA21_14330"
                     /inference="COORDINATES: protein motif:HMM:NF019315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HEX9651737.1"
                     /translation="MINKPKILLLVLAGIFCHSAVAQYDIAITDSIMHNELVLSDLFY
                     YEDPGGEFTFKDISNSKFQKNFKKNPHYIKSDFQPDVSYWIRLKIKHNPESTNLWLLE
                     FYDQTIDEIFCYMPQPGGEFQTQAMGDMFKFPNRKFRHKNFEVILNNSQDSVMTYYFR
                     VKSHETADVRIVVRSFYRFLYHAITEYLFFGLFYGILFSVALYNLLMASVFRDYKYHL
                     YIFYLLSLAVYSASRDGIAFQYLWPDAYQWNQIAVGTSIYAMVLGALLFTRSFLKTDK
                     KFPLLDKVILWWVIIRTLIFMYAMFVDTALFSYRIIEIIPVSIILYSSIAILQKGYKP
                     ARFMVIAHAVLFTGFIIKSLVDSGMIAFNPVTHYSLRIAFVIEMIFLTLALCDRVRLL
                     KITRDQIQRRIIREHEINAELTDKVNKELEQKIKERTLELDEKNSLLEESYKKLSEKA
                     EQINKMNSVLDLDNWKLKKEIRSVLHDRFLNKELSYEDFKWLFPDELSCYRLLEEIKW
                     ENGFLCTKCGNDKYSRGKTKFSRRCTKCGYEESVTVNTVFHGIKFPINKALFIHYLTK
                     EENGKYTFVELSEILNLRPGTISNFKRKIAGITNGHSNEIVLFGPRKVQFNR"
     gene            complement(16078..16151)
                     /locus_tag="VGA21_14335"
     tRNA            complement(16078..16151)
                     /locus_tag="VGA21_14335"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16115..16117),aa:Arg,seq:acg)
     gene            16314..17060
                     /locus_tag="VGA21_14340"
     CDS             16314..17060
                     /locus_tag="VGA21_14340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651738.1"
                     /translation="MMRLTKGLLFLSCFSMISGTLLAQEINQSENTTFRTRYGQPDIP
                     GTFVMDFGFTQMMNYPDTLSNKFWASKSVNFYYYYDFKLGTSGITFSPGIGFAFDRYG
                     FQKSITLANPNEDTVQVVGLPIIQPSWDVNKTKLIINYFEIPLELRWHSNPNSFRRSF
                     YVALGGKFGVLIDNSTKIKYEVNDDKFKVKDKRDLSLNKIRYGFLVRMGYKGFSLFYY
                     QNLSTLFEENKGPLLSDPKSFSIGITMRGF"
     gene            complement(17064..17843)
                     /locus_tag="VGA21_14345"
     CDS             complement(17064..17843)
                     /locus_tag="VGA21_14345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009581404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX9651739.1"
                     /translation="MYNFLKFETDNGTATIILNRPEVYNALNDEITYELQDVLKQIAK
                     DPEARVVVLTGEGKAFCSGQDLKASSGREKRSFKESLHKRYNPIISAIRNMPKPVICR
                     LNGVAAGAGCSLALACDIIIAADDAVLIEVFINIGLVLDSGSSYFLPKLISYSQAFEM
                     STMGSKINGRKAAEMGLVNYSVPMAELDAKVKVFTDFYSKAPTKAVGIIKKQLNKSYT
                     SSMDEMLEYEALTQEIAGSSNDYKEGVTAFLEKRKPDFKGN"
     gene            complement(17854..18468)
                     /locus_tag="VGA21_14350"
     CDS             complement(17854..18468)
                     /locus_tag="VGA21_14350"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX9651740.1"
                     /translation="MSRVAAIISYIFHPLLMTTILFMAMFAINSAPVMPYESGVRQII
                     TVIFLVTFVIPAISISFLRVTKSISSLKLENRKERLIPFFFITIFYGVASWMFLSKLN
                     FTGSINIILITITALILLLTLITLFWKISAHATGVGGVLGFLSAIYLQDPNKESLVSI
                     SAAVIMSGLVLSSRLYLNAHNPRQVYAGLFLGFIFSFASILLFA"
     gene            complement(18468..19901)
                     /gene="rpoN"
                     /locus_tag="VGA21_14355"
     CDS             complement(18468..19901)
                     /gene="rpoN"
                     /locus_tag="VGA21_14355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010611636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HEX9651741.1"
                     /translation="MQKLGLSQTLSQKLSPQQIQFIKLLQVPTAELEARIEEELETNP
                     ALEEGKEENSDDLPEEEYEETDYENDEINVEDYLRDDDFSGYKMQGDGSAFEEREMPI
                     ATSSSLPEQLLSQLGFLGLDERQTSIGTQLIGSIENDGYIRRDLEAIINDLAFSMNIE
                     TSLEEVEAILSKIQNFDPPGIAARNLQECLLLQLKRKEDKEEIHLAIRILSDCFEEFT
                     KKHYQKIMKKLGVEEEELFKLAVDHIIKLNPKPGSSGAGMVKNQYLLPDYILTNNNGK
                     LELTLNSKNAPDLRISHSYSEMFKAYDKSDKKDSKLKETVSFVKQKLDAAKWFIDAIK
                     QRQQTLLNTMQAIIKYQYDFFLSGDESKLRPMILKDIALEINMDISTVSRVANSKSIQ
                     TEFGLYPLKYFFSEGIATDSGEDVSSREVKHSLKELIGGEDKKRPLSDDKLEKLLKAK
                     GYNIARRTVAKYREQLNIPVARLRKEL"
     gene            20018..21466
                     /gene="asnS"
                     /locus_tag="VGA21_14360"
     CDS             20018..21466
                     /gene="asnS"
                     /locus_tag="VGA21_14360"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017733330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HEX9651742.1"
                     /translation="MIAKRKKIALVLESVETGVEVLVMGWVRTKREAKNVIFINLNDG
                     STINNLQVVANPSNFDQSILDKINTGTAVKVNGTLIESGGAGQKVELAASTIEILGVA
                     DPEKFPLQPKKHSLEFLREIAHLRPRTNTFGAIFRIRHAMIFAVHGFFNSKGFLNIHT
                     PIITGSDAEGAGELFKVTTLYLEDIQRNEEGKVDFRKDFFEKETNLTVSGQLEAELAA
                     SALGEVYTFGPTFRAENSNTTRHLAEFWMIEPEMAFYDLEDNMTLAEEFVKSVINYVL
                     ENCSSDLAFLQNREQEEEKNKPQQERNEMNLIDRLKFILNNSFERITYTEAIDILKNS
                     NLNKKKKFQFLIEEWGTDLQSEHERYLVEKHFKKPVIIYNYPKEIKAFYMRQNEDGKT
                     VAAMDVLFPGIGEIIGGSQREERLEHLLSRINELKINAESLWWYLEIRKFGSVPHSGF
                     GLGFERFVQFVTGMSNIRDVIPFPRTPGNAEF"
     gene            complement(21492..21842)
                     /locus_tag="VGA21_14365"
     CDS             complement(21492..21842)
                     /locus_tag="VGA21_14365"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEX9651743.1"
                     /translation="MKRILLFGIVIFLAAQAKAQSETSFLNKDFEKKIQIDIYPNPTT
                     DYLNIQLAGTELNSEVSFHLHNIIGNEVKVEFEKMGDNKYRINVETLSPGYYLLAIKE
                     TNTKFSKTLKFLKR"
     gene            complement(21936..23441)
                     /locus_tag="VGA21_14370"
     CDS             complement(21936..23441)
                     /locus_tag="VGA21_14370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018620998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEX9651744.1"
                     /translation="MKILSAGQIKAADQFTIENEPIRSIDLMERASQQLANWLMQKFD
                     TGKKIIIFCGTGNNGGDGLALARLLSEKKYQIAAYTITKGKKGSADFEQNKARFEKSG
                     TIFDISETSEIPFLSNKEDLIIDAIFGTGLTRKPEGLYADVIEKINGSEATIVSVDIS
                     SGLPCDDMVQNPVAVKPTYTVSFQFPKRSFFFKENAPFVGEWFIVDIGLNKEFIASQK
                     TQFNWLTEDIVKLFQIKRKRHSHKGDFGRCLMVAGEKGKMGAAVLASKACLRAGAGLL
                     TVVVPKCGTQILQTSVPEAMVNENNGAEFLQEQPIRIEDYDAIGIGPGIGQRKETKEL
                     LIQILKGSSKPIVADADALNLMAANQELLEILPRGSILTPHPKEFERLAGKSKNSQEQ
                     VKLQQSFSRKYKVITVLKGGYTSISDSEGNIYFNTGGNPGMATGGTGDVLTGILTGLM
                     AQGYSPLNSALFGVFLHAKAGDLAANHGSQESLIAGDIIENLPLAFRLLSK"
     gene            complement(23486..24115)
                     /locus_tag="VGA21_14375"
     CDS             complement(23486..24115)
                     /locus_tag="VGA21_14375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019989625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HEX9651745.1"
                     /translation="MAEFRSLFPLNLVAFPGEELNLHIFEPRYIQLIDDALADSKKFG
                     IPAYVKSKIEFGTEVEIAEVSKEYEDGRKDIKTRAGRIFRVLNFMNPVRGKLYSGGKV
                     EFIKTDYSSDPSLQIEIEELLADLYQALNIKTSFLEKTERRVFDIAHNIGLSKEQEYE
                     LLKITREDLRQTFVINHLQKAIPLLKEMERSKELIKMNGHFKNFDAINF"
     gene            complement(24159..>25060)
                     /gene="icd"
                     /locus_tag="VGA21_14380"
     CDS             complement(24159..>25060)
                     /gene="icd"
                     /locus_tag="VGA21_14380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004156571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HEX9651746.1"
                     /translation="WFHGVPSPVKDPSTTNMVIFRENTEDIYAGIEFMHGTPEADKII
                     KFLTGEMGIKKIRFPETSSIGIKPVSLEGTERLVRSAIEYALSEGRPSLTLVHKGNIM
                     KFTEGAFKKWGYDLAEREFGNKTFTWAEYDRIVEKEGADTANAAQQKAIDEGKLIIKD
                     VIADAFLQQILTRPAEYSVIATLNLNGDYISDALAAIVGGIGIAPGANVNYITGHAIF
                     EATHGTAPKYAGQDKVNPGSLILSGALMFEYLGWKEAATLIYSGLEKAIAGKRVTYDF
                     HRLMEGATLLKCSEFGKEIVNNM"
BASE COUNT         7265 a         5378 c         4617 g         7800 t
ORIGIN      
        1 agtttaattt ttatccttgg attagcaatg tcttttttgc ttgtaactaa acaagaagcg
       61 aaagctgata cctgttggta atttaaaact atttccaccg aatctcattt tgcaaatcca
      121 tcggaatgtg tccctaaata atcgtcaatt acaattctca atgtcaatct gaacgataag
      181 gcgtcggacg gatacgtctt ttgtccgaac tcaatttctt aagagttaaa aatctaatga
      241 aatgactaga tcgaaaatgt tatccgtcag acgccgggct tagctccttg aataagaact
      301 tgcattgcta cctgattcgc ccggccctat ggataagtaa ttagtcactt ttcaaattag
      361 atatccccga gtccgtcccc aactatcaag taggaattaa tctacatgtc tggattccga
      421 gacagaaata acgcttttca aaaatccagg ttaaatattt gattcaattt gttaaatgag
      481 tctgtatcaa gtacaactca gacgtgttaa tgacttcgta actcgaattc ggcttctttg
      541 gtttcggctg aactggtgcc gacaaacact ttgaatttcc ccggttcagc gccatagctc
      601 atgtcctgtc tgtaaaagga aaggtcatca atagtaattg taaaatgtat atttcggctt
      661 tctcttggtt gaagagttat ttttctgaac ccttttaact ctttaacagg cctggtaaca
      721 cttcctacaa gatctctgat atacaattgt gctatttcac tacccgttac atctcctgag
      781 ttagttatgt ttacactaac atccaatgag tcgtttagcg taatggtact tttacttagt
      841 tctatttcac tatacgaaaa ttgtgtgtag ctcaatccgt atccaaaagg atacaatggt
      901 tcgttaagga catcgaggta tttgctagtg tatttgttac ctgcatcaaa tggtctcccg
      961 gtgtttttca tgttatagaa aatgggtatt tgccctacat tcctcggaaa ggtgatcgtt
     1021 aattttccgg aaggattatg gtcaccaaat agaacatcag cgattgcgtg ccctgccatt
     1081 gttcccaagt gccatgcatt aagcactgca ggcatgtttt cagcaatcca ggctatcgtc
     1141 aatggtctac cggccataac aattaccacc accggtttcc cagtattggc aatttcttca
     1201 accagctgtt gctgaagacc gggaagatcc aattctgatc gactggctgc ttcaccactt
     1261 tgttgataat tctcaccaac tgcaaggata accacctctg attgctttgc gattttaaca
     1321 gcttcagaaa acccagacct atcctcacct gtaaatccgc atccatgaac aacattaact
     1381 tctgcatcag gcgtcgcctc cctgacaccc tcaagtaatg acaccacctt acttgcatca
     1441 cccgcagcat gccatgtgcc taattggtct tctttattat ctcccaacgg accaataatc
     1501 gcaattcgct taatagattt tgacaaggga agtattttgc gtccttcaaa ttcttcattt
     1561 ttaagcaaga caattgattg ccttgcagtt tcccttgcat gcgacaagat ttcttctgaa
     1621 taaattacct ccttttctct taaagtgtct gtatacctgt atggatcatc aaataatcct
     1681 aactgaaatt ttgcggtaag tatccttcgg acagaggcat caattgttgt ttcagaaact
     1741 ttaccttcct ttacagattc agataaatgg ttcagaaata cagacccctg catatccata
     1801 tcaatacctg cgttgactgc aagctccccg gcatttttta aatcaaatgc aaatccgtgt
     1861 ggtatcattt catttattcc cgtataatca gaaaccacaa aaccctggaa tctccattca
     1921 tttctgagaa tttctgtgag aagaaaatga tttccagtgg ctggaatacc gttgagttca
     1981 ttgaacgatg ccataactga accagagcct gcatcaatag ctgctttgaa caaagggaga
     2041 taaaattctc tcaatgtgcg ctcagacaaa tcaactgtat gataatctct tcctgcctgt
     2101 gcagctccat aagccgcaaa gtgtttcacg caggcgagca tggttgaatg atctgaaaga
     2161 tcatcaccct ggtatccacg aactttagca gcggcaatta aacttcccag ataagtatct
     2221 tctcctgaac cctctgaaat tctgccccag cgcgggtccc ttgcaatatc taccattgga
     2281 ttaaagttcc aattcaatcc ggatgcagtt gcttccatag cagaatacct cgccgctttt
     2341 tgaatgatgt ctaaatccca agtacatgct tccgcaaggg gaatgggaaa aatggtttta
     2401 tgcccatgga tgacatcgga tcccaataat aatggaatct tcattcttga ctcttcaacg
     2461 gcaagcctct gcaattccct attgtaaccg gctgtgtgag cattgaatat attgccagcc
     2521 ttgccttcaa caatatattt tctgttttct tctcccaatg ttggtcccgt aacgctccaa
     2581 tgacttgtga aaagggtaag ttgaccaatt ttttcttcca gtgtcatcag attcaaaata
     2641 gaatctactt tatcctgata aggatttgca tcggcaattg gtcgtgagcg gtctgcacag
     2701 ccaaaaaaca aaattgaata aataattaca tgtaagcgga cattaaacat gggctgatga
     2761 tggttagtaa gttccactat atttattatc aaaccttttc ccctggtctc tccttagagg
     2821 tatttgcatt ccgttggtct ttttaagcca gtcaaaaagt tcctgattca tatctgcaat
     2881 gatgtcctgg tattcctcat tccgaattaa attatttgat tccaaaggat cattctgaag
     2941 atcaaaaaat tcattaatat cccatattcc ctggtagcga atgaacttat attgatcaga
     3001 tcttactgca aaaacagttg gagtttgcgg aaaggggcgt tcccagaaat attcataaaa
     3061 taatttatcc ctccatggta tttcctcacc caataacatt ctggaaaatg gcataccatc
     3121 catgtttgat ggcatcttta atcctgccag ttcaagtatg gtaggtgcaa tgtcaatatt
     3181 ttgtatcacc ggcttaataa tggttcccgg tttaatgttc gcaggactaa atgccaataa
     3241 tggcactctc atggattctt catacgcttg tcttttgtct atcaacccat gttcaccaaa
     3301 agcaaaacca ttatccccca tatagaaaat gattgtagat tccattagtc ctgcctcttc
     3361 aagatattct attattcttc cgatgctttc atctactgca aggagtgttt cgttataacg
     3421 gtaataaaaa ttctcaaaat ttatgttgcc atggtacatg taatccactc catgccagct
     3481 atagcgctgc tttttcaccc agtccggcac atctttgtag ttatatattg aatcattgtt
     3541 aattccttca gctcttggat catcagatcc tggtggaaac atcgttaacg gatattgaat
     3601 cggtttcccc tgatactttc cattatgcct tttgggaggg tgaaattccg aatgaaccgc
     3661 tttgtgtgag aggtacatga agaaaggttg ttctgaggtt cttgttttaa gccagtcaac
     3721 tgcataatct gtaagtaaat cggttatgta cgtactgtcc ttatattcaa ttctttctcc
     3781 atcgacattc attgcaggat tgtaataggt tccctgtcct ctaaagctta cccaacgatc
     3841 aaaccctttt cttggctcat cgttatcatg gcccatatgc cacttgccaa caaatgcagt
     3901 ttcatagcct gcccgttgca gatactgagg aaagaagttc acggaatctg caattggcga
     3961 ctggttatca acgacaccgt gatgatgagc gtactgacct gttaatattg aagcgcggct
     4021 tggtgagcat aacgatgtgg taacaaatgc attctgaatg tgggctcctt gtaatgccat
     4081 cttgtccatg ttaggggttt gcaaaaaagg aaccttaccg gtaaagccca taaaatcgta
     4141 acggtgatca tcgctaagta taaatacgat attgagcggt ttattattgt ttgccttttc
     4201 caaaacaaac tcatcatgag tatttccact acatgcctgg ataagtatga taattatgat
     4261 gaatgataca atatttttat tcattgactt gttgacttta accattcaaa tttaactgca
     4321 attaataccg gtgtttttca ttataagtaa atccgagttt tgctaaaccc tgcttcacct
     4381 cttcagaaga catcagcaaa tcccaaatta atcctgttcg gtgattttca atcattacaa
     4441 caatgggacc ctggtcaata gccagatatc gttgcggaaa ccaatcgtct tgaatactaa
     4501 atgcatcata aaaaccatac tcacccaaaa gcttatctcc aagttcctca tagaaaaagc
     4561 gcatggcttg catggaatat tctggagtat agggaaatga cgacaaggct gctgtgggtg
     4621 aaattacccc caggtctctc ccaggactat gcccggcata ccctttcggc gaataagaag
     4681 atgtaagccc ccaacaattt tcaccatacc cctcaaaatt catttgattc tcaatacaat
     4741 attgacggtt tatgagcgta tgatttctgt tcagttccca gtagtcggcg tattgatctt
     4801 ttaagccacg gggatcgatt cccaaatagg aataatgcgc ccagaaaagt ggaccgccat
     4861 attcttccgc accgttatga ctgagtatta atttgtatcc atatttttca tttgcagagc
     4921 gtatttctcc tgatcttgcc caaccttcat gatagacttc ggcaggaatt ccatgggttg
     4981 gtgaagctgc ggccattaca tacatgatca aacattcgtt ataacccttc accggaaaat
     5041 tcatttccca ctgatattgt ggtgaccaat gccagtacaa aacgttctca tcatttttgc
     5101 gataccagtc ccactccact tctttccaaa gggtatccaa tttcgcagca agttccattt
     5161 ctgaatcatt accttctctg aaatattgtc ttgcagctat caacccctga acaaggtatg
     5221 cagtttcgac caggtcgcca ccattgtctt tggggctgaa aggtttggct ttgccggttt
     5281 caccgtacag ccaatgcgac catgctccat gaaatctttc ggcatttttc aaatagtctg
     5341 caatgtgatt taatcgctca aagccttgtt gacgggtgat gaaacctctt tcaattccaa
     5401 ccaggattgc catgattcca aaccctgagc cgccggatgt aatcaccgtc tgatcatttt
     5461 gcggataaat tccatccatg tgaattcgtt cccttgccaa cccggagaca ggctccgctc
     5521 catcccaaaa atattggaaa gtgcgatact gaatgagggt cattagggag tcgtcggaaa
     5581 ttgccctgct gttattgata tcttccgaag tagagctttt accccccgaa ttgcaggata
     5641 caaaaacgac aaagaatact gagacaccaa agcaaaaaga gtccaatttt atcttcatat
     5701 caagcttata aaattttcaa tttgtaaaat ccagcttatt cattgctgtc tgaacttccg
     5761 gtgctgacat aaacaaatcc cataaaagtc ctgttctata attttcaatc ataattatga
     5821 taggtccctg atcaattgca atagttgaag tcgcggtcca cccctcagtt atattatacg
     5881 catcgtagaa tccgtatggt ccccaaagtc tgtctcccag attataataa aaaaacttca
     5941 aggccttcat cgattgttcc ggagcataag gaaaggatga aagggcggcc gtgggagtaa
     6001 ttactcccag atcatttgtt ggagaatggg cactataacc aatttgattg tcgctagcag
     6061 taagtcccca attaagatca ctgtagccaa caaaatcttt agggttgtct aagcaataag
     6121 cctgatttat gagtgtatga tttgcatttt ggaggaaata atcactgcaa tatgtatcac
     6181 gcaaatttct tggatcaaga ccgaggaatg aataatgcga gaaaaacaac ggacctccgt
     6241 aggcctgtcc gagaggaagg ataatgttgt aatatgagtt tccattgaca atatcaccgt
     6301 taattgcata tcccaaatga taagcagaag catcgatggc atgagttgga gatgctgcag
     6361 caagaaaata cgttattaat gtttcattat ggcctctgat ttgatgattc atattccaac
     6421 cgtggttcgg ggaccaatgc cagtataaaa cattttgccc tccctgggta aaccaatccc
     6481 actccacttc ttcccagagc atatttattc gggttattaa atcattctcc tggagtacat
     6541 tactattaag atattgcctg aaggtgagta aaccctgaat cataaatgat gtctcaacca
     6601 ggtcacctcc gtcgtcttcc gtaccgaatg ggattacaga accggttgtc ccatccagcc
     6661 aatgggacca gactccatgg aatcgatctg cggtctcaag gaaattaaga atttgagaca
     6721 acctgtcgat tccttgttgt cttgtaatga accctctttc tattgctact attattgcca
     6781 tcactccaaa accggatcca cccgttgtaa ccaggcttcc gctaatattt ctttctctgg
     6841 ccatcccact tacaggttcg gcaaagtccc agaaatattt aaaagtttgc tgttgaacca
     6901 atgtgagaag atcttcatca cttattaccg gaagcaccgg ctcggtgttt attgcactat
     6961 aaaagtaacg attcatctga gtaaatgact cgccgttcaa tcccgtaatg tcacccgata
     7021 tccatagacg gtaacgggtt aaatgtctca atggctgact aatggtaagg gtaaccttct
     7081 ttttatcttc agagagcaaa atcgaaagtg gtatgttctg gatacccaat attttgaaat
     7141 aatctccgga catataatcc ggatcaaccg gtttcgaaaa cataatttca atcgttccaa
     7201 caagtggtat atccgtaatc cttgagttat taataacttc tttctcactc attaaaaggg
     7261 aggtgatgtt cattgttcct ggttttgttg aaaaatcaac aactactccg ggaaaaactt
     7321 cgccatcatt acctcgcaaa gtattggcta tttcgagctg gaaattatcc attgatgaca
     7381 ggtttgtaga aggggtagcc gaaaacgtct tgtaattatc ctgaaaggaa aaatttaggg
     7441 aataagattc accagaaacg gattgaagtg taacggaatt ttttaatgat gtagtatcta
     7501 atgaagcgga gaaactggcg attataggct gatcgtaagg tgtttcggaa tttagttgct
     7561 gatcgtccag gtcaagttca tagctgccta cacgaattga gttaagttgt atggtaggcg
     7621 gactaatctc catatctttt ttgcatgagc acaaaagcag gattaataaa gaaggtctta
     7681 atatgtaaga gatacctctt ttcacaattc cgattagaac aagagggttc ccttactacc
     7741 agggaaccct cttaaaaata aaactagttt aacttattac ttttcagaat tatacatcaa
     7801 gtctatttct gttaatgtca gcgttttaaa atagattctt atgtcgtcca gttcaccctg
     7861 gaaatggttt gctgatgcaa aatcataacc accccatgtt tcattatccc aaagtgtacc
     7921 tgccctgctt tgaatgaagc caaaagccag ttcgttaaca acttccggtg ccactccgtc
     7981 atactcaagc cctgaaacat atgttttatt atcaccttca ggccacaaat caaaatcctg
     8041 ctctttcatt ttctcgccat ttatgtatgc agttcctgtt ttttctacag cattgtacac
     8101 caatgctaca tgcgtccatt ggtctttgat tactgcttcc aggccaccag atccggataa
     8161 gtctttagaa aatgtccatc cttgccaacc cagattaccg gtagcgtcta cccagaggtc
     8221 ctcacttgca gtacccgggc catttgttgg atggttatat ccataccttg ctgccagctt
     8281 gcaattggaa tagtctccag ctatttcaaa ttgaaaccca taaaatgcgc cgaggccaag
     8341 gacaaagtgt ccactcgttt tgccatttgc agagctcggt tttacccaaa agcttagtgt
     8401 ccaactcccg tcgttcatca agtcatctcc attggagatc tcaattatac ttgtggagcc
     8461 attaaaagat gctgctttac ctgcagtggt gtttcttgaa gcaacatagg taatgtctac
     8521 cacctgactt gtggttggat cgaaggctcc aatcacatca ttagcattgt cctcgaaggt
     8581 ccaatgcgcg acagcaccag caggcgcaaa agctccatca gtcgtgaaat ttctttcaat
     8641 tccctgcgta agtacttttc cttctgcaga cgtaaggccc ggtgcaattg ctaacacaaa
     8701 tagagttcca gttccgaaat catcaacagg atcaatggta actgtagatc caacgacaga
     8761 tacattcaga ttcatcgtgg tgttatcata atctcttgtt aagataacag ccaaggtact
     8821 ttcattaatg cttgtattaa attctgccgt aatggttgca cctggcggaa catttgtcgg
     8881 acttgttgca gcattcagat caattccatc tgctgtaaga ctaaccaaag ccagttctac
     8941 cggctcatca tctttacagc tcataagcaa agctgttgtc atcccaaaaa tcgttgcgat
     9001 gcttagaatc caattcactc ttttcaattt cataattcta tactttttaa gttaaacgtt
     9061 tgtttaattg ttttattgaa gtgtattttc attaattcat tattcatttt accatccggg
     9121 attttgttct aatcttccct gggtgaggtc aatctcgtcc tggggcaatg gtaacaattc
     9181 atctttattt atggtgaatc cgagggggcc taaaactgat tgagcctggc ctgttcttaa
     9241 aagatcccag aaacgttgac cttccaaagc taattcaact cttctttcat tgtagataat
     9301 atcgcgtaat aatgtttgat cggtttcggt aatgtcaggc aggatagtat tattcccctg
     9361 ccttgctctc tccctcacca aattaagata cacgagggct tggccaggat taccaacttc
     9421 atttaatgcc tcagctgcca ttaataagac atctgcatat cgaattattc tccggttatg
     9481 cccgtgggac gggggtacgt ttgagccggg agtccagact ttctgattgt acgtctctaa
     9541 ttctatattt ccatggctgt cggttgtctc atctggtgtg gaaccatcgc ccataatggg
     9601 tactccatct aatacttcac ctaaaaagat tattgtagca tccatccttg gatcgttggg
     9661 ttcgaaagca ttcatcaaat ccattgtggg cctgttgaaa ccccatccac gattaggagt
     9721 tcctctcact ccctgaacat ttgcatattg attacctcca ttttggatac cctcctgacc
     9781 aaaagctcca acttcaaaaa cggattcaat tccatgttct ccaagaacac tatttgcatc
     9841 ctcaaaattc atttcgagat tatattcgcc ggaattgatt acttcaagag taagacttgc
     9901 agcatcggca aaattttgct ggaacaaata cactcttgcc aacagggatt ttgctgcacc
     9961 tttagttgcg cgtccaaggt cggccggatt tccatagccg gatttttccg ggagattagc
    10021 aattgcaaac agcaaatcat cttcaattaa agaaaaaact tccgcctttg ttgccctggt
    10081 agcatctgca gatggctcgg tggatgttat gatcggaact cccccccagg ctctgaccat
    10141 atcaaaataa aagagcgcac gaagaaagct ggcttctgca atgtttcggt tctgaagagt
    10201 taagtccatt gctatatcag gtactatttc aatcacaaca tttgcccttt ttataccttc
    10261 gtagagtgtg ttccaccagc gtaggaggga tccttctgtt gcaacgtgtt caaatcgatc
    10321 atacggaccg attgcattag ctgcatcatc cggattactt cctttattgg catcatcaga
    10381 catgatatcc aggattggaa ataatccaga attgaaatta ggaattcgca aagtattata
    10441 caccgcgttg actgccaatt cagcatcgtt agcagtggtg ggaaatgttt cttgagttaa
    10501 catgccttgc ggaggttttt cgagaaaatc ttcacaggat gaaagtatca acaaagaaat
    10561 caaccctccc ttttttatta ccctgactat gcttatataa atcttcatta ttcttaactg
    10621 ctttaaaagt tcacattaag cccaatggaa aaaatctgcg aaaccgggta tccgccaaag
    10681 tcaattccat ttagattaac aatctcgctg gatacttctg gtgagtatcc cgtgtagtcg
    10741 cttatcgtaa aaacatttgt tccactgaaa tagactttgg cagactggat tcttgctctg
    10801 ctgattaggc ttgttggaaa attgtaagcc agggtcaggc tgcgtagcct gaaaaacgat
    10861 ccgttgtaaa taaaatggga ggagggatta ttaaaattaa cacctcccga acttggcctt
    10921 ggatctgtat ttgttgaacc atcgccggtc cagtaattga aatatctttg ctcaaaattg
    10981 taaagatcag gtctaaccgt ttctttgtta ttatatattt catttcctgc ctggccctga
    11041 aaatcaatgc ttagttcaac tcctttatag cccacttcca aattaagacc atagacgaag
    11101 tccgggattg gagagccgat cattgttctg tcgtcaccat ttaatacacc atcggcatta
    11161 acatcacggt atcttaaatc gccaacgcca gtattattca gatgtggata tgagttcaat
    11221 tcagctgaat tttgaaaaat gccatctaca atgtaaccat agaaagcacc aattggaagg
    11281 ccaggagcag accgcgttac ctgtccgttt gcaaacccgc caagttcatc atcgctccct
    11341 ccggtccccc tgactttcac agtttcgtta tgtatagttg tcccattagt tcctattcgg
    11401 taatttatgc tgcttgtttc accacggaat gccaaaatgt attcaaggcc cttgtttaca
    11461 acttcaccgg cattgaaaaa tatagttgct ccatctccat tcccaaagta gccgggtacg
    11521 agcaagggaa tcagtatatc ctttgttcgc ttgttatagt aatccaactc tcctattaat
    11581 tgatctccaa agaacccaaa ttcaaaccca atatcagtct gatgtgtgtt ctcccatttt
    11641 atgcctgaat tccctgaaat accataggat gatccgggat attgaacatc ctcagtacca
    11701 aatacagcat tcaatccgtt ggaaatcaag gaaaattgac ttgaatatct tatcttttcg
    11761 ttcccgacta taccccagct agctcttaac ttcaagttgg aaagaaatgg aatctctcgt
    11821 aaaaaattct cgttaatcaa attccaccca acagcaaaag acgggaaatt tgcatatttg
    11881 ttttccttag tgaatttcga ggatccatcc cttctgaaag ttgcagtaaa cagatattta
    11941 tctttcaaag tatagttgaa acgagccagg taggaaatca ttgagaaatt cagatcgtca
    12001 tttactccat tgagaatacc gttagggtta atattattgg gattaatata ccagaaatcc
    12061 tggtcgggcc ttagcagatt ttcacctacc agtgtcactt gctctgagga tgactcttgc
    12121 atggtaaaac caattaccgc atccaaataa tgaggccctg tttcattgtt aaagcttaaa
    12181 gtattttccc acaaccaatc agttttaaaa aaataattct tggtcaaatc atcggtggaa
    12241 ttctcttgtt gagagttgac aaagtagatg ggtgtaaagc gcttggattt tacattatca
    12301 atatctactc cgaaagagct tctaaacttg atccctttta gaaaatctac atcgccataa
    12361 aagttaccca ctgtacgcag gccattgtcg aaactatttg tatactcaat gtctgctaag
    12421 acatttccga cccctggcac aggcgtatat gtaccatccg gttgaaaagg ctctaaggtt
    12481 ggccaggctc tgtacgctac aaagacggca ttcccattgg tattctgtct tttataaggt
    12541 gtaactgcga tattgttacc tattttgatg aaattggtta tattgtagct attgttcagt
    12601 ttcagcgtta ccctttcgta attagacttg gttataatac cctcctgatt aaaatacccc
    12661 attccgatat agtagctggt tttttcgctt gctccggaag cggagatctg atagtgctgc
    12721 attggggcga tatcaaaaat cagatcctgc cagtcagtgt caggaaccag atcaacatta
    12781 ttgtaactcc cggatttgat ttggttggcg acaatggcaa actcccgccc atttaacaaa
    12841 tcaatttttt tctcaagttt ctgaatgctg tattcagcgt tgaatgaaat tactggttca
    12901 ccgagtttac cttttttggt agttatcatt accacaccat tagcacctct tgaaccgtaa
    12961 atagctgttg ccgaagcatc cttcaatata tcaacggatt cgatatctgc cgagttaaga
    13021 aaatcaatat cctgaacaat aacaccatcc acaacataaa tcggcgctgc aacattaaat
    13081 gtaccctgac cccttatccg aacaatcaca cttgaacccg gtgcgcctga tgcactagac
    13141 acttgtacac ccggcacttt accttgcaaa gattgcatag gattggatga agtaactttt
    13201 cccagatctt tgccacgaat agaggaaacg gacccagtta aatcagactt ccgcaccgta
    13261 ccataaccga taacaactat ttcttgcaaa atatcaatgt cttcttcaag cgtaatatca
    13321 attgtcgtct gattattaac agccatttcc tgggtgacat agcccacata tgaaaatatc
    13381 aacatggaat ttgtaggttc actaatttct attgtatatc taccgtcaat gtcggttaca
    13441 gtaccaatgt tggttccttt gattaccaca tttaccccgg gaagagtttc gccggttcca
    13501 gcttctttta cctggcctct tactatcact ctttgggcct gcgtctgctc agggaaaaag
    13561 agaataattg ccagtgaaaa ggttagcacg cgatttaaag gggtcataaa tctgaacata
    13621 accggtggtt tttactgtct aaaagaaata ctttgattta agtccgcaat tccaaggccg
    13681 ggaatcctaa tttgaagttc attttctcaa ccttatattc ctcagatcaa ctcttactaa
    13741 aatttactta ttagttatat atccgattta cttatgctac taattccgct aaatttttca
    13801 ggattttctg tggtttacct atgttaattt tatcacaata atctcaatag aaatttgctc
    13861 tactttaaaa ttagcaatgt tttagggatt tcccctatcg aataaccaca aaatgccaga
    13921 gactgttcag attatctatt gaattgaact ttgcgagggc cgaatagcac tatctcattt
    13981 gagtgtccgt ttgtgatgcc tgctattttc cttttgaaat ttgaaatggt gcctggtctc
    14041 aaattgagaa tttcagataa ctcaacaaag gtatatttgc cgttttcttc ctttgtgagg
    14101 taatgaatga acagtgcctt attgatcgga aatttgattc catggaaaac tgtgttaact
    14161 gttacagatt cttcataacc acactttgta catctcctgg agaatttagt tttacctctt
    14221 gaatatttgt cgttaccgca ctttgtgcag aggaaaccat tttcccactt gatttcttct
    14281 aataaacggt aacagctaag ttcatcagga aaaagccatt tgaagtcttc ataactcaat
    14341 tccttattta agaaacgatc gtgtaatacg ctccgaatct cttttttcaa tttccagttg
    14401 tcaagatcaa gtactgaatt catcttattt atttgctcgg ccttttcaga aagcttttta
    14461 taagattcct caaggaggct gttcttttca tctagttcca acgtccgttc tttaatcttt
    14521 tgctctaatt ctttgttcac tttatctgtt aattctgcat taatctcatg ttcacgaatg
    14581 attcttcttt gaatttgatc ccttgttatc ttaagtaacc gcactctgtc gcataaagcc
    14641 aaggtcagga atatcatttc tatcacaaat gcaattctta aactataatg cgtaacaggg
    14701 ttgaaagcaa tcattcctga atcaactaat gatttaataa tgaaaccagt aaacaaaact
    14761 gcatgagcaa ttaccataaa acgagctggc ttatacccct tttgcaatat tgcaatgctt
    14821 gaataaagaa ttatcgaaac cgggataatt tcaataattc tatagctgaa taaggccgta
    14881 tcgacaaaca ttgcatacat gaaaatcaga gttctgatga taacccacca taggataact
    14941 ttatccagta atgggaattt tttatcagtc ttcagaaaac ttcgggtgaa aagtaaggcc
    15001 ccaagtacca ttgcataaat tgatgtgcca actgcaatct gattccactg gtatgcatct
    15061 ggccacagat attgaaaagc aattccgtca cggctggctg agtatacagc aagactcaga
    15121 aggtagaata tatataagtg atacttataa tctcgaaaga cagatgccat aagtagatta
    15181 tataatgcta cactgaagag tatgccatag aataatccga agaacaaata ctccgttatt
    15241 gcatggtaaa ggaaacgata aaaagaccga accacaattc tcacatcggc agtttcgtga
    15301 gactttaccc taaagtaata agtcattacg ctgtcctggc tgttgtttaa gattacttca
    15361 aaattcttat gtctgaattt ccggttagga aatttaaaca tgtcacccat tgcctgggtt
    15421 tggaattcac cacctggctg tggcatataa caaaaaattt catcaatggt ctggtcatag
    15481 aattcaagaa gccaaaggtt ggttgattca ggattgtgtt tgattttcag tcgtatccag
    15541 tagcttacgt caggttggaa atcgcttttt atataatgag gattcttttt aaaatttttc
    15601 tgaaattttg aattggagat atctttaaag gtgaattctc ctccaggatc ttcatagtaa
    15661 aataaatcgc tcaataccaa ctcattatgc atgattgaat cggtaatggc aatgtcatat
    15721 tgtgcaaccg cactatgaca aaaaatacca gcaagaacga gcagaagaat tttgggttta
    15781 ttaatcacct ttcactatga tatggtcacc aaggccaatt tagtaaagct attttgaaat
    15841 gcggaattct aaggtgaata aagagctaac ttgttttatg ttgcacatta ttcggtatag
    15901 cttttccttt atcaattgta atatgacttt ttcttagact caagattaga tatgattaga
    15961 tctcaaattg aaggattgca aaaaaaccaa tgcctaatac accgagaaaa actccaagaa
    16021 tatcacagtt tacagatttt actctaaact cttaactcca aactctcaac tcatcagtga
    16081 acccggaggg agtcgaaccc ccaacctcct gatccgtagt caggtgctct atccagttaa
    16141 gctacgggtc catttcttta ctttaaatac atttcaaaat cgctgcatcg gtccggtttt
    16201 tgagaaggca aaagtaagca tattcaccaa aaattcgccc gtatatccta attagtcaaa
    16261 aaagtttatc tttgccagct aagtttgaag tggtgggtat tgaatttttt cttatgatgc
    16321 gattaacaaa aggactactc tttttatctt gtttttctat gatttcaggc actcttttag
    16381 cccaggaaat caaccaatca gaaaatacaa ccttcagaac gcggtatggt cagcccgata
    16441 tccccggcac ttttgtgatg gattttgggt tcactcagat gatgaactac cccgatacac
    16501 tcagcaataa attctgggct tccaaatcgg ttaattttta ttattactac gatttcaaac
    16561 tgggaacgag tggcatcaca ttcagtccgg gtattggctt tgccttcgat cgatatggtt
    16621 ttcaaaagtc gataacactg gccaatccca atgaagatac cgtccaggta gttggcctcc
    16681 ctatcattca gccatcctgg gacgtcaaca aaaccaaact catcattaat tattttgaaa
    16741 tccctttaga actgcgatgg cattccaacc caaacagttt ccggagaagt ttttacgtcg
    16801 cattgggagg taaattcggg gtgctcattg acaactctac caagatcaag tatgaagtga
    16861 atgacgacaa gtttaaggta aaggacaaaa gagatctcag ccttaacaaa atacgctatg
    16921 gtttcctggt cagaatgggc tataagggat tcagcttatt ctattaccaa aatctttcaa
    16981 cactattcga agaaaacaaa ggtccgctgt tgagtgatcc aaaatcattt tcaatcggca
    17041 taactatgcg cggattctaa aatttaattt cctttgaaat ctggttttct cttttcaaga
    17101 aaggctgtca caccttcttt ataatcattt gagcttccag caatctcctg ggttaatgct
    17161 tcatattcca gcatttcatc cattgaagag gtataggact tgttcaattg cttctttatt
    17221 atgcccactg cctttgttgg cgctttcgaa taaaagtcag tgaacacctt tacttttgca
    17281 tccaactccg ccattggaac agaataattt acaaggccca tttccgcagc cttcctgcca
    17341 ttgatctttg aacccatagt gctcatttca aaggcttgtg agtagctgat tagcttcggc
    17401 aaaaaatatg aggagccgga atccagcacc agtccgatgt taatgaatac ttcaatcaaa
    17461 acggcatcat ccgcggcaat aattatatca caagcgagag ccagtgaaca gcctgccccg
    17521 gcagccacac cattcaacct acaaatcacc ggcttaggca tattacgaat tgcactgatt
    17581 atcggattgt atcttttatg cagtgattcc ttgaacgatc ttttctctct accggaggac
    17641 gctttcaaat cctgccctga gcagaatgcc ttcccttcac ctgttagtac aacaacccgg
    17701 gcttccggat ctttggcgat ttgtttcaac acatcctgaa gttcataagt aatttcatca
    17761 ttcaatgcat tatacacttc aggccggttt aaaataatag ttgccgtacc attgtcagtt
    17821 tcaaatttca aaaagttgta catgatatat cgtttacgcg aaaagtaata ttgaagcaaa
    17881 actaaaaata aatccgagaa ataatcctgc gtaaacctgc ctgggattat gggcattaag
    17941 ataaagccgc gatgacaata ctaagcccga cataattaca gcggcggaaa ttgatacaag
    18001 gctttccttg tttggatcct gcaagtaaat tgcagaaagg aagcctaaca ccccaccgac
    18061 tccggtagcg tgagcgctta tcttccaaaa aagggtaatc agagtcaaca gcagaattaa
    18121 ggcagttatg gtaatgagaa taatatttat tgatccggta aaattgagtt ttgaaaggaa
    18181 catccaactg gcgactccat aaaatattgt tataaagaag aacggtatta accgttcctt
    18241 tctgttttcc agcttaagcg atgaaatgga ttttgtaaca cgcaaaaatg atatacttat
    18301 ggctggaata acaaacgtga ccagaaaaat cacagtaatt atttggcgga caccactctc
    18361 ataaggcata acaggcgctg aattaatggc aaacatagcc atgaacaaaa tggttgtcat
    18421 cagcagagga tggaaaatat aggatatgat tgccgcaact ctgctcacta taattctttt
    18481 ctcagcctcg caaccggtat gtttaattgt tccctgtatt ttgccacggt tcgcctggcg
    18541 atgttatagc cttttgcttt caaaagtttt tcaagcttat catcagacaa tggccttttt
    18601 ttgtcttcac ctcctatcaa ctcctttaat gaatgtttta cttctcttga acttacgtct
    18661 tcgccggaat cagttgcaat tccttcggag aaaaaatact tgagagggta taatccaaac
    18721 tcggtttgta tacttttgct gtttgctacc cgagacaccg tagaaatatc catgtttatt
    18781 tccagtgcaa tatcttttag gatcatggga cgcagtttac tttcatcgcc tgatagaaaa
    18841 aaatcgtatt gatattttat tatagcctgc atagtattca ggagggtttg ctgtctttgt
    18901 ttaatggcat caatgaacca ctttgccgca tcaagtttct gcttgacaaa agaaacggtt
    18961 tccttcagct ttgaatcctt cttatcactc ttgtcatagg ctttaaacat ttcagaatag
    19021 gaatgactta ttcgaaggtc gggagcattt tttgaattca gagtaagttc cagttttcca
    19081 ttattattgg ttaaaatgta atcgggcaac agatattggt tcttaaccat tcccgctccc
    19141 gaacttccgg gtttcggatt caatttgatg atatgatcga cagcaagttt gaataattct
    19201 tcctcttcca cccccagctt cttcataatt ttctggtagt gtttttttgt aaattcttca
    19261 aagcaatcgc ttaaaatgcg aatggccaaa tgaatctcct ctttgtcctc ttttcgcttt
    19321 aactgcaaaa gaagacattc ttgtaaattt cgcgctgcaa ttcccggagg atcaaaattc
    19381 tgaatctttg acaagatagc ttctacctcc tccagactgg tttctatatt catagagaaa
    19441 gccaggtcgt taataatcgc ttccagatct ctccggatat acccgtcatt ttctatactt
    19501 ccaatcaact gcgtgcctat tgaagtttgt ctttcatcaa gtccaagaaa gcccagttga
    19561 gataaaagct gctcaggaag agaggaagaa gttgcaattg gcatttcacg ctcctcgaat
    19621 gccgacccat ccccttgcat cttataacca ctgaaatcat cgtcgcgtaa ataatcttcg
    19681 acgtttattt catcattttc gtaatctgtc tcttcatatt cttcctctgg aagatcatcg
    19741 gaattttcct ctttaccttc ttccaaagct ggattggttt ctaactcttc ttctatacgg
    19801 gcttccagtt ccgcggtagg aacctgcaac aatttaatga actgaatttg ctgaggagac
    19861 agcttctgac tgagggtctg agataagcct aatttttgca ttgataattc tttccaacgt
    19921 gcacagatta ccttactttc aaagttatat tattttacct aatctttgat aaaaaggcca
    19981 gaatatcgtt tttttgcgca cttatcagaa ccgatgtatg attgctaaaa gaaaaaagat
    20041 tgccttggta ttggaatctg tggaaacagg agttgaagtg ctggtgatgg gctgggtgcg
    20101 aacaaagaga gaagccaaga atgtaatttt cattaacctc aacgatggct caaccattaa
    20161 caatctgcaa gtagttgcaa atccttcaaa tttcgatcaa tcaattcttg ataaaatcaa
    20221 cacgggtact gctgtaaaag ttaacggtac gttgatcgag tctggtggtg caggtcagaa
    20281 agtggaactt gcggcatcaa caatagaaat tctgggtgtt gctgatcctg aaaagtttcc
    20341 gcttcaacca aaaaaacatt cgcttgaatt tttacgagaa atcgcacatc tacggccacg
    20401 cactaataca tttggagcaa ttttcaggat ccggcacgcg atgatttttg cagtccatgg
    20461 tttttttaat tccaaaggat ttctaaacat acacacacca ataattacag ggtctgatgc
    20521 ggaaggtgcc ggtgaattgt tcaaagtaac tactttgtat cttgaagata ttcaacgtaa
    20581 cgaagaaggt aaagttgatt tcagaaagga tttttttgaa aaagaaacga accttaccgt
    20641 ttcaggtcag ttggaagcag agctggcggc gagtgcattg ggcgaggttt atacatttgg
    20701 cccaactttt cgcgcagaga attcaaatac aacccgtcat ctggctgagt tttggatgat
    20761 tgaacctgaa atggcgtttt atgatttgga agacaacatg accctcgcgg aagagtttgt
    20821 aaagtcagta attaattatg tattggaaaa ttgcagttcg gatttagctt tcctgcaaaa
    20881 cagggaacag gaggaagaaa aaaacaagcc acagcaagaa agaaatgaaa tgaaccttat
    20941 cgacagatta aagttcattc ttaataattc ttttgaacgt ataacttata ccgaggccat
    21001 cgatattctt aagaattcaa atttaaataa gaaaaagaaa tttcaatttt taattgaaga
    21061 gtggggaaca gacctccaat cagaacatga aagatacctg gtcgaaaaac attttaagaa
    21121 accggtgatt atttacaact atcctaaaga aattaaggca ttttacatgc gtcagaatga
    21181 agatggaaaa acggttgctg ctatggacgt tttatttccg ggtattggtg aaattattgg
    21241 cggatcacaa cgtgaagaaa gacttgaaca tttgttgtca cgaattaatg aacttaagat
    21301 aaatgcagaa agtttgtggt ggtatttgga gattcgaaag ttcggttccg tgccccactc
    21361 aggttttgga ttaggatttg aaagattcgt acaatttgta accggcatga gcaacatccg
    21421 cgatgtaatt ccatttccac ggacacctgg aaatgctgaa ttctgatttc aacacaaaaa
    21481 attttagaga tttatcgctt aaggaacttc aaagttttgg aaaactttgt attagtttct
    21541 ttaatagcca gaaggtaata gcctggagaa agtgtttcta cattgatgcg gtatttattg
    21601 tcacccatct tttcaaattc aaccttcact tcatttccaa ttatattatg aaggtggaat
    21661 gatacttcag agtttaattc agtacccgcg agttggatat tgagataatc agttgtcggg
    21721 ttgggataga tatcaatctg aattttcttt tcaaaatcct tattcagaaa cgaagtttcc
    21781 gactgcgcct ttgcctgggc tgccagaaaa atcactattc caaataataa aatccgtttc
    21841 atcgcgttga gctgctcaat aatcagacca ataacggcct tatcctttgt taaaaatacg
    21901 taaataatga atgctttgtt taattaatcc gggaattatt tggatagtaa ccggaacgcc
    21961 aaaggtaaat tttcaataat atcacctgca attagagact cctgagagcc gtgatttgct
    22021 gccaggtcac ctgctttagc gtgaagaaaa accccaaaaa gagcggaatt taagggagaa
    22081 tagccctggg ccatgagccc tgtaaggatc ccggttaata catctcctgt gccaccggtt
    22141 gccattccgg ggttgccgcc ggtattgaaa taaatattcc cttcggaatc ggaaatagaa
    22201 gtgtacccac cctttaggac agtgatgacc ttatattttc gggaaaatga ttgctgcaac
    22261 tttacttgct cctgggagtt tttggactta cctgctaacc tttcgaactc ctttggatgc
    22321 ggggtgagaa ttgatcctct ggggagaatc tccagcaatt cctggttagc agccatcaaa
    22381 ttcagggcat cagcgtctgc cacaattggt tttgaagatc cttttaaaat ctgaatgagg
    22441 agttccttgg tttctttcct ttggcctatt cccggaccaa tgccaattgc atcataatct
    22501 tcaatccgga tgggctgctc ctgcaggaat tccgcgccgt tattttcgtt aaccatagct
    22561 tcgggaactg aagtttgtaa aatctgagtt ccacatttag ggaccactac cgtaagcaga
    22621 cctgcgcctg ctcttaagca agctttactg gctagtacag cagcacccat tttccctttt
    22681 tctcccgcaa ccataagaca ccttccaaaa tcacccttgt gactgtgacg tttcctctta
    22741 atttgaaaca gtttaacaat atcttcggta agccaattaa attgtgtttt ttggctggct
    22801 ataaattcct tattcagacc gatatcaact ataaaccatt caccaacgaa cggagcattt
    22861 tctttaaaga agaaagatcg cttaggaaat tggaaactga cagtatatgt tggttttact
    22921 gctacgggat tttgtaccat atcatcacag ggcaaaccac tggaaatatc aacagaaact
    22981 atggtagctt ctgagccgtt aatcttctca ataacatctg catataaacc ttccggcttt
    23041 ctggttaatc ccgtgccaaa aattgcatca attatgagat cctccttatt agataaaaag
    23101 ggaatttcag atgtttcaga gatatcgaat atggttcctg atttctcaaa ccttgctttg
    23161 ttttgttcaa aatcagccga tccttttttc cctttagtaa ttgtataagc agcaatctga
    23221 tatttttttt ccgaaagtaa tctggccaac gctaatccat ctcccccatt atttccggtg
    23281 ccacaaaaaa taattatttt tttaccagta tcaaattttt gcattaacca gtttgctagt
    23341 tgctgcgatg cgcgttccat cagatctatg gatctgatgg gttcgttttc aatggtgaat
    23401 tgatcggctg ccttaatttg ccctgcagat aaaatcttca taatgcaatg cagatgagtt
    23461 accagtaaat atggcgcgaa ttaagttaga aattaatcgc gtcaaaattc ttaaaatggc
    23521 cattcatttt aattaattct ttgcttcgct ccatctcttt tagaaggggt attgcttttt
    23581 gcaaatgatt aattacaaaa gtttgacgta aatcttccct ggttattttc agtaactcgt
    23641 attcctgttc ttttgataat cctatattat gagcgatatc gaatactctt cgctccgtct
    23701 tttccaggaa actggtttta atatttaggg cttgatagag gtctgcaagt aattcctcta
    23761 tttcaatttg aagagaagga tctgaggagt aatcagtctt aatgaattcc acttttccac
    23821 ctgagtaaag tttaccgcgt accgggttca tgaaatttaa aacacggaaa attctgccgg
    23881 ccctggtctt tatgtccttt cgcccatcct catattcttt tgaaacttca gcaatttcta
    23941 cctccgtgcc aaattcaatt ttgcttttaa catacgcagg gattccgaat tttttactat
    24001 ccgcgagggc atcatcaatt aactgaatgt aacgtggttc aaaaatatga aggtttaatt
    24061 cttcaccggg aaaagcaacc agattgaggg ggaaaagaga tctaaattct gccatgggaa
    24121 aatccgaaag ggaggtcaat cttcatttac ggaacttatt acatattgtt tacaatttcc
    24181 ttcccgaatt cagaacattt tagcaaagtg gctccttcca tcagcctgtg aaaatcataa
    24241 gtaactcttt tgccggcaat tgctttctcc aggccggagt agataagagt tgctgcctct
    24301 ttccatccta aatattcaaa cataagcgca cctgacagta tcaaagaacc gggattcact
    24361 ttgtcctgtc cggcatactt cggcgcagtt ccgtgagttg cttcaaaaat tgcatgaccg
    24421 gtgatatagt taacattagc tcccggagca attccgattc cgcccacgat cgctgccagg
    24481 gcatcagaaa tataatctcc atttaagtta agagttgcaa taacagaata ctctgccggc
    24541 cttgtcagga tttgctgaag aaaagcatcg gcaataacat ctttaattat tagtttacct
    24601 tcatcaattg ctttttgttg agcggcatta gcagtatccg ccccttcttt ttctacgatt
    24661 ctgtcatatt cagcccaggt aaaagtttta tttccaaatt ccctttctgc caaatcataa
    24721 ccccactttt taaaggcacc ttcagtgaat ttcataatat tacctttgtg aaccagcgtt
    24781 aaggagggtc gtccttcgct aagcgcgtat tcaatcgctg atcttaccag gcgttctgta
    24841 ccttcaagcg aaacaggctt tataccgatg gatgaggttt caggaaacct gatctttttg
    24901 atgcccattt ctcctgtcaa aaactttatt atcttatcag cttccggagt tccatgcatg
    24961 aattcgatcc ccgcataaat atcttcagtg ttttcacgga aaataaccat gttagtagtg
    25021 ctgggatcct ttacaggtga tggcacacca tgaaaccatc
//
LOCUS       DASWHX010000219         2372 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_252834, whole genome shotgun sequence.
ACCESSION   DASWHX010000219 DASWHX010000000
VERSION     DASWHX010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 2372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2372
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_252834"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(120..1886)
                     /locus_tag="VGA21_14385"
     CDS             complement(120..1886)
                     /locus_tag="VGA21_14385"
                     /inference="COORDINATES: protein motif:HMM:NF028237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HEX9651747.1"
                     /translation="MNLQPNTFAIILLISGLIELILSVVSYQSGTSASKWFSVVMFFI
                     SIWAIAYGFELMCSSLDQMIFWINIEYIGIAFLPSAWIIFTIKFVTKEHWLTPWFYIA
                     VFIYSAIIVGLVWTNESHHLHYSEVGLSTTGPIPLLVITTGLSYHIHTIYFYLILLIG
                     LALFLDKFRKSNRYYQNQTIIILFGMLIPWIANFIYLIGLRPYEHIDMTPFGFVLTSL
                     FIWYGISRLKLFDILPVARSKLLERMQEGVLVVNQEDKIVDMNSAMKKFLSKGNSQLY
                     GKLIYKILPGVARPYWALGEKSSGKFETSISVNNKVTHFEVTVIHLEDNHKNLLGTLS
                     VFKDITERVQSHEELMRQSEELKNLNQLKDRLFTIIAHDLRGPLNSFLQLLSVQQAGE
                     ITDDQFKKILPVVTLEVGYTSSLLENLLQWSISQIKESKVSPQKINLNSIASEEFNLF
                     IKKANEKGIDLINNVESDIMVYADEEMVRLIMRNLVANAIKYSKKNDLIKVSAAKEDG
                     QIITSISDNGMGMDAAKMQEIIDGTVISSPGTDREKGTGLGLILCQDFVRRNNGRFWI
                     ESEVGKGTTVYFSLPVSSVGGR"
     gene            2034..>2372
                     /locus_tag="VGA21_14390"
     CDS             2034..>2372
                     /locus_tag="VGA21_14390"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007658045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX9651748.1"
                     /translation="MLHIVRTDSGNLDFVTLVKQLDADLAARDGEIDHAYYEQFNKID
                     KIEFVVVAYENDLPVGCGAIIAHERGAMEVKRMYVTPAKRGQGISTKILIELETWAAE
                     LSFNKCILETG"
BASE COUNT          743 a          464 c          422 g          743 t
ORIGIN      
        1 ttcgactccg gtaataaatt cattaaaatc attacccgga tcatttatga cttccaatac
       61 ctttggctga attttttata atcaagtact ctaatttctt taatttcttc aggagtaatt
      121 tatcttcccc cgacactgct aacaggcaaa gaaaaataaa cggttgtgcc ttttccaact
      181 tcactttcta tccaaaatct gccattattt cttctcacaa aatcctggca taaaatcaaa
      241 cccaaaccgg tacccttctc tctatctgtg cccggacttg agataactgt tccatcaatt
      301 atctcttgca ttttcgcggc atccatgccc attccattat cacttattga agtaattatt
      361 tgaccatcct ctttggcggc acttactttt atgagatcat tctttttaga atatttgatg
      421 gcatttgcga ccagatttct cataatcagc cgaaccattt cctcatctgc ataaaccatt
      481 atatctgact ctacattgtt gattaagtct ataccttttt cattcgcttt tttaatgaac
      541 aaattaaact cttcggaagc aattgaattt aagttgattt tttgggggct gactttactt
      601 tcttttatct gacttatcga ccattggagc aaattctcaa gtaaggaaga ggtgtaaccc
      661 acttccaggg taactaccgg taaaatcttt ttgaattgat catcagtgat ttcacctgcc
      721 tgctgcacgc ttagcaattg taaaaagcta ttgagagggc ctcttaagtc atgtgctatt
      781 attgtgaaca accggtcctt cagttgattg agatttttca attcctccga ttggcgcatc
      841 aattcttcat gggactgaac cctttccgta atgtctttga acaccgaaag cgtaccgagt
      901 aaatttttat ggttatcctc cagatgaata acggtaacct caaaatgtgt gactttattg
      961 tttacactaa ttgaagtttc aaactttcct gaggactttt ctcccaacgc ccaatatggt
     1021 cgtgcaacac caggtaaaat tttgtatatg agttttccat aaagttgaga atttcctttg
     1081 gacaggaatt ttttcatggc agagttcatg tctactatct tatcttcctg gttcaccacc
     1141 aacactcctt cctgcattct ctctaataat ttactcctgg caaccggaag aatatcaaaa
     1201 agttttaacc gggaaattcc ataccagata aaaagagaag ttaacacaaa accaaaaggt
     1261 gtcatgtcaa tatgttcata gggcctaagt cctattaagt aaatgaaatt ggcaatccag
     1321 ggaataagca tcccaaagag aataataata gtttggttct ggtaatacct gttggatttt
     1381 ctgaatttgt ccaggaacaa ggcaaggcca atcagtaata tcaggtagaa ataaattgta
     1441 tgaatatgat aggataagcc ggtagtgatg acaagcaggg gaatcggacc tgttgttgac
     1501 aatcctactt cactataatg aaggtggtga gattcgtttg tccagactaa tccaacaata
     1561 attgccgagt aaataaatac ggcaatgtag aaccatggag ttaaccaatg ctctttagtt
     1621 acaaatttta tggtaaatat tatccatgct gagggtagaa atgctatacc aatatattca
     1681 atattaatcc aaaaaatcat ttggtcaaga ctggaacaca tcagttcaaa tccataggct
     1741 atcgcccata tagaaatgaa aaacatgacc acggaaaacc acttggaagc tgatgtgccc
     1801 gactgataac taaccacgga aagaataagt tcaatgagtc cggatattaa cagaatgatc
     1861 gcgaatgtgt ttggctggag attcataaat acggtaacta cttgctttag ctaaccaaat
     1921 ttaacataat ctgccatttg acctttaaat taaaagaaat atggacgctt gttatcttgg
     1981 ttgtcattca gaaattaatt ttttgatatt taactttcaa gctgcaaatc aatatgctgc
     2041 acattgtaag aactgattcc ggaaacctgg actttgtgac cctggtaaag caacttgatg
     2101 ccgatctggc tgcgagagat ggcgaaattg atcatgccta ttatgaacaa ttcaataaaa
     2161 ttgataaaat agaatttgtg gtagtcgctt atgaaaatga tttgcctgta ggctgcggag
     2221 cgataatagc tcatgaaaga ggtgcaatgg aagtaaagcg aatgtatgtg actcccgcga
     2281 aacgcggtca aggcatttct acaaaaatcc tgatagaact ggaaacctgg gcggcagaat
     2341 tatctttcaa taaatgcatt ttagagaccg ga
//
LOCUS       DASWHX010000220         3319 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_1360720, whole genome shotgun sequence.
ACCESSION   DASWHX010000220 DASWHX010000000
VERSION     DASWHX010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 3319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3319
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1360720"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..507
                     /locus_tag="VGA21_14395"
     CDS             <1..507
                     /locus_tag="VGA21_14395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009581990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX9651749.1"
                     /translation="SRSRHRTMVPIEEYENGHILTSQSCKKLTDAGVKVNLGAHGQLQ
                     GLGAHWELWMLSQGGMTNMEVLRAATINGAEYLGMDSEIGSIETGKLADLIILELNPL
                     EDIRNTSSVNYTIINGRVYDTSTMNEIGNTNRPRTPFWWEMAGYSEKFDWHAITHGIA
                     TPGCICGY"
     gene            complement(508..1527)
                     /locus_tag="VGA21_14400"
     CDS             complement(508..1527)
                     /locus_tag="VGA21_14400"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX9651750.1"
                     /translation="MHSYLSTISSSLHQLIGNLNWNIGRILSDSKFLLLTIQSQTGEE
                     ELLAEQAEQIEDLKMWIVAFLIVAFVIIVVMTIVIQSNRILRRQNTTIKSQQEEISRI
                     NNNLAIQVENLKELNSEKNSVISYVSHDLITPLSNIEGLVNLVSLKKENLSSEQEEYL
                     KKMRDVVKNGKETIQSMLNINKIEQEFKGIDLMEHEAISLIEEVLLGYQYQMDEKKIN
                     VRKEYILESIILNTDKQYFKQIIGNLLSNAIINTPENEEITISCGVVDGNVKIKINDN
                     GEGLSEKDKERIFLKYTQSRGVGNQQGISLAIVKQLVDKLNAKIRVESKPGHGTQFIL
                     EFSGH"
     gene            1641..2921
                     /gene="tyrS"
                     /locus_tag="VGA21_14405"
     CDS             1641..2921
                     /gene="tyrS"
                     /locus_tag="VGA21_14405"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017729836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEX9651751.1"
                     /translation="MGNFIDELKWRGMIHDIMPGTEELLDSGMCSGYIGFDPTAPSLH
                     IGNLVQIMMLVHFQRSGHKPYVLVGGATGMIGDPSGKSEERNLLDEESLRINESCIKK
                     QLEKFLVFEEISNHAVMVNNYDWFKDRGFLEFLRDIGKHLTINYMLAKESVKNRIEAG
                     ISFTEFSYQILQGYDFLYLYQNHGVKFQMGGSDQWGNITTGTELIRRKANGEAFALTT
                     PLITKVDGSKFGKTEEGNVWLDGEMTSPYKFYQFWLNCSDEDSTKLIKIFSIKGKEEI
                     FDLESKHRNAPHERILQQVLAEEITARVHSEEELEQAKIASRILFGKSTKEDLESLDE
                     KLILSVFEGVPQTEISKGNLNQDILQFLSEGTNGVVFKSKAEARRMIEGGGLSFNKTK
                     IDSIEKAPEYNLLQGKFLLIQKGKKNYYLIKVVD"
     gene            complement(2924..>3319)
                     /locus_tag="VGA21_14410"
     CDS             complement(2924..>3319)
                     /locus_tag="VGA21_14410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008201852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HEX9651752.1"
                     /translation="RDHHDVSGTDSPFRETSNIYDGSRFTADMAVQNVIGDSFRGATW
                     VSIHNGGGVGWGEVINGGFGMYLDGSETAANNIRNMLFFDVNNGIARRSWARNEGAVS
                     AIKKEMLRTPGLKITLPNSVNEDDLKSLL"
BASE COUNT         1026 a          626 c          655 g         1012 t
ORIGIN      
        1 agcagatcaa ggcaccgaac aatggttccc attgaagaat atgagaatgg acacattttg
       61 accagtcaat cgtgcaaaaa actaaccgat gccggagtta aggtaaattt gggagctcat
      121 gggcaattgc aggggttagg agcacactgg gaactttgga tgctaagcca gggaggaatg
      181 acgaatatgg aagtattaag ggcggcaacc ataaatgggg ctgagtacct gggtatggat
      241 tcagaaattg gtagcattga aaccggcaaa cttgctgatc taattatttt ggagttgaat
      301 cccctggaag acatcagaaa tacttcttcg gtaaactata ctattataaa tggcagagtt
      361 tatgacactt caaccatgaa tgaaattgga aatactaata ggccgaggac tcccttctgg
      421 tgggaaatgg ccgggtattc agaaaaattt gactggcatg caatcacaca tggtattgca
      481 acaccgggat gtatctgcgg gtactagcta atgtccacta aattccagaa tgaattgagt
      541 gccatggcca ggtttacttt caaccctgat tttggcattc aatttatcca ctaactgctt
      601 cacaattgcc aaggatattc cctgctgatt ccctacgcct ctgctttggg tatactttaa
      661 aaaaattctt tccttatctt tctcacttag tccctcccca ttatcattaa ttttgatttt
      721 cacgtttcca tcaaccacac cacaggaaat tgttatctct tcattttcag gagtgtttat
      781 tattgcatta cttaataagt tgccgattat ttgtttgaag tattgtttgt cggtatttaa
      841 aataattgat tccaggatgt actctttacg gacgttgatt ttcttttcat ccatctgata
      901 ctggtaccct aaaagaactt cctcaataag agaaattgct tcatgctcca ttaaatcaat
      961 tcccttaaac tcttgttcta ttttattgat attgagcatg ctttgaatag tttcctttcc
     1021 attcttaacc acgtcccgca tcttcttaag gtattcttcc tgctcagaag aaaggttttc
     1081 ttttttcaat gatacaaggt tcaccaggcc ctctatatta ctcaatggcg taattaaatc
     1141 atgagaaaca taggatataa cagaattttt ttctgaattt aattctttaa ggttttctac
     1201 ctgaatagcc aggttattgt ttatccggga aatttcctcc tgttggcttt taatcgtagt
     1261 attctgtctt cttagaatcc ggttagactg aattacaatt gtcatcacaa cgataattac
     1321 aaaggccaca atgagaaaag ctactatcca catcttcaga tcctctattt gttccgcctg
     1381 ctcagccaga agttcttcct cacctgtttg agattgtatg gtaagtaaaa gaaattttga
     1441 atcagataga attctgccaa tattccagtt caaattccca atgagctggt ggagtgatga
     1501 agatatcgta ctcagatacg aatgcatgaa acattttcaa ttaagcctta aaataaggag
     1561 ttattttata aattaaaaat ctctttaaag gcatttaatt cacaatacaa tatctttgcc
     1621 atcagcttaa aatgggggca atggggaatt ttattgatga actgaaatgg cgtgggatga
     1681 tacatgacat catgcctgga acagaagaat tattggattc tggcatgtgt tccgggtaca
     1741 taggatttga tcccaccgcc ccttctctgc atattggcaa tcttgtccag attatgatgc
     1801 tggtacattt tcagcgatcc ggccataaac cttacgtgct tgttggcggt gcaaccggaa
     1861 tgattggaga tccatccggt aaatcagaag aaagaaattt actagatgag gaaagtttgc
     1921 gcattaacga atcctgtata aagaaacaat tggaaaaatt tcttgtgttt gaggaaatca
     1981 gtaatcatgc ggtaatggtc aataattatg actggtttaa agaccggggc ttcctggaat
     2041 ttctcagaga tattgggaaa catctcacca tcaattatat gttagcaaaa gaatccgtga
     2101 aaaacagaat agaagcggga atttccttta ctgagtttag ttatcagatc cttcagggct
     2161 atgattttct ttacctctat caaaatcatg gtgtgaaatt tcaaatgggt ggctcggatc
     2221 agtggggtaa tattactacc ggtactgaat taatccggag gaaagcaaat ggagaagctt
     2281 ttgcattaac aactcctctt ataaccaagg ttgatggttc aaaatttggg aagaccgagg
     2341 aaggtaatgt ttggttggat ggtgaaatga catcacctta caaattttat caattctggc
     2401 ttaattgttc ggatgaagat tctaccaaac tcattaaaat atttagtatt aagggtaagg
     2461 aagaaatctt tgatttggaa agtaaacata ggaatgcgcc acatgaaaga attttgcagc
     2521 aagtacttgc ggaagaaatt actgccaggg tacattcaga agaagaactg gagcaagcta
     2581 aaattgccag tagaattcta ttcgggaaat ctacgaaaga agatttggaa tccctggatg
     2641 aaaaattaat tttatcagtt tttgaaggag ttccgcaaac cgagattagc aagggaaatt
     2701 tgaaccaaga tatcctgcaa tttctatcgg aaggtacaaa tggagttgtc ttcaagtcta
     2761 aggctgaagc aaggagaatg attgaaggag gcggacttag ctttaacaaa acaaaaatcg
     2821 attcaataga aaaagctccg gaatacaacc tgctccaggg aaaattcttg ttgatccaaa
     2881 agggaaagaa aaactattac cttatcaagg tagtggatta accttataaa agagatttaa
     2941 ggtcatcttc atttacagaa ttgggcaatg taatcttgag cccaggcgtc cgcaacattt
     3001 ccttctttat cgcggaaacc gcaccttcat ttcttgccca cgatcttctt gcaattccat
     3061 tatttacatc gaagaaaagc atgtttctta tgttatttgc agcagtctca cttccatcca
     3121 gatacatgcc gaaccctcca ttaattactt cgccccaccc tacgccgccg ccattatgaa
     3181 tggaaaccca ggtagctcca cgaaatgaat ccccaatcac gttttggacg gccatatctg
     3241 cggtaaatct ggatccatca taaatatttg atgtctccct gaatggtgaa tcagttcctg
     3301 aaacatcatg gtgatctct
//
LOCUS       DASWHX010000221        10191 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_1770436, whole genome shotgun sequence.
ACCESSION   DASWHX010000221 DASWHX010000000
VERSION     DASWHX010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 10191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10191
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1770436"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(122..610)
                     /locus_tag="VGA21_14415"
     CDS             complement(122..610)
                     /locus_tag="VGA21_14415"
                     /inference="COORDINATES: protein motif:HMM:NF025474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility lipoprotein GldH"
                     /protein_id="HEX9651753.1"
                     /translation="MIRIHNFFIIFLLAGVLISCDSKRVFETNENFSQKYWHKDSLKS
                     FSFHIPDHTSTYSLYMNLRITASYPFQNIYIQCQLKDSLNKAFEDQLLNFQLFHSQTG
                     EPFGSSGVGDIFDHQFKVFENYKFPASGGYSLHFAQMMRKDTLNDIMSVGARVEFFQP
                     EN"
     gene            complement(594..1751)
                     /gene="ricT"
                     /locus_tag="VGA21_14420"
     CDS             complement(594..1751)
                     /gene="ricT"
                     /locus_tag="VGA21_14420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013455461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HEX9651754.1"
                     /translation="MGCSTCKSGGVVAGCRNNGGCLTGGCNKMNVFDWLTDMEIPASQ
                     KFDVVEVRFKSGRKEFFRNAESLDIITGDPVVVDVPNGHHVGYISLQGELVRLQMQKK
                     GITNDDQVRKIYRLATTKDLEKLATVQNRESNTLYRTRQIIKDLGLEMKLTDVEFQGD
                     NSKATFFYSAEDRVDFRELIKMLATEFKIRVEMRQISLRQEAGRIGGVGSCGRELCCS
                     TWLSDFKSVTISAARYQKLSLNPTKLSGQCGRLKCCLNYELETYIDALKDIPEVEEPL
                     VTEKGNAVLQKTDIFRKLMWFGYESENTWHPVDVSRVVEIQSLNKQGKKPATLLEDTD
                     FAREEKVRAISRDLERLDKKFADKGRPKKRKNKKRKNRNPNNRQRPNDKNS"
     gene            complement(1812..3098)
                     /gene="purD"
                     /locus_tag="VGA21_14425"
     CDS             complement(1812..3098)
                     /gene="purD"
                     /locus_tag="VGA21_14425"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011583703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEX9651755.1"
                     /translation="MMNILVIGSGGREHALSWKLSQSNLCEQLFIAPGNAGTEGVGKN
                     IPLNPDDFASVATFIKKNNVNLVVVGPEAPLVNGIRDFLENDSELKGLMIIGPGKSGA
                     RLEGSKDFSKEFMVRHGIPTGKAMTFHKENIEEGIVHLKTLKAPYVLKADGLAAGKGV
                     IITESFEEARSIFKDMIQNKMFGEASTKVLLEEYLDGVELSVFVLTDGESYKILPEAK
                     DYKRIGENDTGPNTGGMGAVSPVPFADKILMEKIEKRVVIPTIKGLKSDGIPYIGFIF
                     IGLMIVNGNPYVIEYNVRMGDPESQVVIPRLNCDLAELFQATSRHTLNDFQVNFDKRA
                     ATTVVMVAEGYPGPYNKGKSIEGLDLESNWLTFHAGTEKKDGQIVTNGGRILAITGMG
                     ESMQTALSRSYQGVSMISWEGEYHRNDIGQDILAMS"
     gene            complement(3144..5321)
                     /gene="recQ"
                     /locus_tag="VGA21_14430"
     CDS             complement(3144..5321)
                     /gene="recQ"
                     /locus_tag="VGA21_14430"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009580052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HEX9651756.1"
                     /translation="MLIEQDDILKEKLKEIFGYSQFRGNQIPIIRNVLDKKDTFVIMP
                     TGAGKSMCYQLPALMSEGVAIVISPLIALMKNQVDQLSALGVNAQFLNSTLTKTEMNK
                     VKKDTLDGFVKLLYVAPESLTKEENVSFLKKAQISFVAVDEAHCISEWGHDFRPEYRR
                     IRQIITQIGDLAIIALTATATPKVQIDIVKNLNIETSATFKSSFNRKNLYYEVRPKQH
                     TKKQLIRFIKENKGKSGIIYCLSRRKVEEIASLLNVNDIKAAPYHAGLDSSVRERNQD
                     AFLNEEVDVIVATIAFGMGIDKPDVRFVIHYNVPKSVEGYYQETGRAGRDGLEGKCVM
                     FYSPGDISKLEKFNKDKSVQERENAAILLLEMVSYAESSICRRKQLLHYFGESFDGNC
                     DACDNCLHPKEEFDGEEFVWTALDAVRQTKERFGIDHLVDVIRGVESQYVKSYNHDDL
                     DIFGKGDEHSAEFWKSVVRQTLLHELILKDIDNVGVLKLSDKGKDFLENPVSLKLTKD
                     HEYSDLGEEEEPDQAPMASACDEELFEILKALRKKVAKEKGFPPYVIFQDPSLDEMAT
                     IYPTSKEELARINGVGMGKVNKFGDVFMEAIQKYVKENDITTANDVVVKSAINKSKLK
                     IYIIQAIDRKIDLDEISETKNTTPEEVLKEIENIIYSGTKLNLDYYINQVLDPERQED
                     LMEYFLNAETDDLQVAMDDDANQDYSEEEIRLMRIKFLSEYAN"
     gene            5392..6360
                     /locus_tag="VGA21_14435"
     CDS             5392..6360
                     /locus_tag="VGA21_14435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009580051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HEX9651757.1"
                     /translation="MNIAKNIKKIAKGVLLNEANSIRAIAGFIDDNFAACVQEILEIK
                     GRVIITGIGKSAIIAQKIVATLNSTGTPSVFMHAGDAIHGDLGIIQPEDMVICLSKSG
                     NTPEIKVLVPLIKRRGVKLAALVSNTNSFLAKQADYILNATIAEEACPNNLAPTTSTT
                     AHMAVGDALAVCLLRLRNFSSEDFAMLHPGGSLGKQLYLKVEDVFPGNELPVVSPDTP
                     LKEVILEISSKRLGATAVVNKKQNLLGIITDGDIRRMLEKQANIKSTKAADIMTKNPK
                     TVSVGDFAVHALSLMQKKNITQVVVVDNKKVVGFVHLHDLLKEGLV"
     gene            6361..7440
                     /locus_tag="VGA21_14440"
     CDS             6361..7440
                     /locus_tag="VGA21_14440"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HEX9651758.1"
                     /translation="MAKQKPVVLIMAGGSGTRFWPYSRDKHPKQFIDILGSGESLIQA
                     TFRRAKKITEKNRIFVATNIKYKKLVLKQLPEITESQILLEPRKKNTAPCITYASMIF
                     QKKFGDVATVVTPSDHTIVREKEFIKVLQHGIRIAENNHGLVTIGIKPTRPETGYGYI
                     QTDKSTKPVKKAVAFREKPSRKKAAEYVRSGNYLWNAGIFVWKNSTIRNELEKFQPKL
                     INDFKQVEATLTTGNTNLKELKKAFEKCQDISIDYAVMEKAGNVSVIPGDFGWSDLGS
                     WNAVFDLSKKDKNGNVIASNSIVEKTKNSIIIGSSKKLIVVEGLNDFLVADHENAILI
                     CRKDNEKEIREFVNRIAKEKGKKFT"
     gene            complement(7433..8173)
                     /gene="rlmB"
                     /locus_tag="VGA21_14445"
     CDS             complement(7433..8173)
                     /gene="rlmB"
                     /locus_tag="VGA21_14445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009580179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEX9651759.1"
                     /translation="MKESDHIYGIRAIIEAIHAGKEVDRIFIQKNARNELIKELIQLA
                     RANNLPMVQVPQEKLDRITKKNHQGAVCLLSGITYASLDHILSQTFENGKIPLIAILD
                     RITDVRNFGAIVRSAEGAGVHAIVVPSRGNAPTSADAIKTSAGALHHLPICRSENLKE
                     TISFLKDSGLRIIGCTEKAEVLHFNENLKDPVALLMGSEDDGISPEYLKMCDGMIKIP
                     MLGKIASLNVSVSAGIIFYEAIRQRFTM"
     gene            complement(8170..9381)
                     /locus_tag="VGA21_14450"
     CDS             complement(8170..9381)
                     /locus_tag="VGA21_14450"
                     /inference="COORDINATES: protein motif:HMM:NF040439.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HEX9651760.1"
                     /translation="MMLSYLLCISLFFKMDPGTAESLADQNLSYLYNRNSEMDIKCRL
                     IYSGESSELLLHIKMRKDVYEMTDYYVFEEYVNSYSDKIGNTSDTVQILDYIIKRTRG
                     GFNFRIKLNPNLQNKIYVLHFANRHNQKHYYYWVPLSNTRKSSILIYDSENLPVIRDW
                     ISNENPVNIVSTQKDSLLFAYHYQIDFEPADPPMMTESSGNASLSIDSLFSVGAGNSI
                     SLTSQGLYFIQSDTTSLHGLGIRVQNSYFPELAHINDLINSLRYFSTRSEWDKLNTAI
                     NKKDALDNYWLSIFRSSEMAKSVIRNYFDQVEEANSLFTNYKEGWKTDMGMVFIIMGP
                     PDEVFRNQAKEEWIYQKTEDLPKMKFTFEKINNIFTDNHYVLIRNADYRNEWYRAVEM
                     WRKGRYETLIR"
     gene            9465..>10191
                     /locus_tag="VGA21_14455"
     CDS             9465..>10191
                     /locus_tag="VGA21_14455"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009580181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX9651761.1"
                     /translation="MGIFTTEITSDKILSDNPIHQRLLKAYRLAAPFVNGDVLELGVG
                     EGRGLKLLAPSAGSYTALDKLKSSIEILSAQYPEVRFIHSSIPPVKDLESNSFDLVIS
                     FQVIEHIKNDRLFLEEIGRLLRPGGKCLISTPNIKKTLTRNPWHIREYTREELYALAK
                     PIFGQIDIKGITGSEKAWKYYQQNKESVARIMKYDIFDLQHRLPAPLLRIPYEILNRI
                     NRNKLQAKDNELVSQISEADYMLS"
BASE COUNT         2992 a         2160 c         1919 g         3120 t
ORIGIN      
        1 cgcaaacccg cgatttgcaa atcatgtggc cggataaatc agtatttcaa tcctttgatt
       61 attttacaga gagaaaagtg tacgtcacgc tggggagacc ggtttcggga aaacttactc
      121 tttaattttc aggctgaaaa aattccaccc gtgctccaac cgacataata tcgtttaggg
      181 tatctttacg catcatttgc gcgaaatgta aactataacc tcctgatgca gggaacttat
      241 aattttcaaa aactttgaac tgatgatcga aaatatctcc aactccgctt gaaccgaaag
      301 gttcgcctgt ttgtgaatga aacagctgaa agttcagaag ctgatcttca aacgctttat
      361 tcaaagagtc cttcaactgg cattgaatat aaatattttg aaaaggatat gaagcagtaa
      421 ttctcaggtt catataaaga ctatatgtag aagtgtggtc tggtatgtgg aatgaaaaag
      481 atttaagtga atctttatgc cagtattttt gggagaaatt ttcattggtt tcaaaaaccc
      541 ttttagaatc gcatgatatt aatacgcctg ctaataggaa gatgataaaa aaattatgaa
      601 ttcttatcat taggtctctg cctgttatta gggttccgat tcttgcgttt tttgttcttc
      661 cgttttttgg gcctgccttt atctgcaaat tttttatcca gacgttccaa atcacggcta
      721 atcgctctga ctttttcttc acgagcaaag tcggtatctt caagcagtgt agcgggtttc
      781 ttgccttgtt tattcaggct ttgaatttcc acaacgcggg atacatccac agggtgccag
      841 gtgttttcgc tctcatatcc aaaccacata agctttctaa agatgtctgt cttttgcaaa
      901 accgcatttc ctttttctgt cacaaggggc tcttcaactt caggaatatc tttcagagca
      961 tctatatacg tttccagctc atagtttagg cagcacttaa gtcgtccgca ttgaccggaa
     1021 agtttagtgg ggttcaggga aagcttttgg taacgggcag cacttattgt gacactttta
     1081 aaatcgctca accaggttga gcagcacaat tcccggccac aacttccaac accgccgatt
     1141 ctgcctgctt cctgccgtaa acttatttga cgcatctcca ccctaatctt gaattcggtg
     1201 gcaagcatct taataagttc ccgaaaatca acccggtctt cagctgaata aaagaaagtg
     1261 gctttggaat tgtcaccctg gaattctaca tcagtcagtt tcatttcgag ccccaggtct
     1321 tttatgattt gacgagtccg gtataaagta tttgactccc gattctgcac tgttgcaagt
     1381 ttttctaaat ccttcgtagt tgcaaggcgg taaatttttc tcacttgatc gtcgttggtg
     1441 atacctttct tttgcatttg cagccttacc aactcaccct gcaaagagat atagccaaca
     1501 tgatggccat tgggtacatc cactacaacc ggatcgcctg ttataatatc cagactttcc
     1561 gcattgcgaa aaaattcctt tcttcctgac ttaaaccgca cttcaaccac atcgaacttc
     1621 tgtgaagcag ggatttccat atctgttaac caatcaaaca cgttcatctt gttgcagcca
     1681 ccggtaagac agccgccgtt attacgacaa cctgcaacta caccaccgga tttacatgta
     1741 ctgcatccca tattttatct atttctttca gacaaggagt aaaattaagg taaatagagc
     1801 taatagtaaa gtcaggacat tgcaaggatg tcctggccaa tatcattcct gtgatattcg
     1861 ccttcccaac tgatcatgga cactccctgg taagaccgcg ataatgctgt ctgcatgctt
     1921 tcgcccattc cggtgattgc cagaatcctt cccccattgg ttactatctg accatctttt
     1981 ttttcggttc cggcatgaaa ggtgagccaa ttggattcca gatcaaggcc ttctattgac
     2041 tttcctttgt tatatgggcc ggggtatcct tcagccacca ttaccactgt ggtggcagcc
     2101 cgtttatcaa aatttacctg gaaatcattc agcgtgtgcc ggctggttgc ctgaaatagt
     2161 tcagccaggt cacaatttaa tctgggtatt accacctggc tttcaggatc acccatcctt
     2221 acattatatt caattacata cggattacca tttacaatca tcaatccgat aaagatgaat
     2281 ccaatatatg ggattccatc agacttcaat ccttttatgg taggtataac tactcgcttt
     2341 tctatttttt ccatcaaaat tttatctgca aatggaacag gacttactgc acccatcccg
     2401 cctgtgttcg gtccggtgtc attctcgcca attcgcttgt aatcttttgc ttcaggaaga
     2461 attttataag attcgccatc ggtcaaaaca aaaaccgata gttcaacccc gtcaaggtat
     2521 tcttcgagta aaacttttgt gctggcttcc ccgaacattt tattctgaat catatcctta
     2581 aaaatgctcc gcgcttcttc aaaggattca gtaatgataa cccccttgcc tgcggcaagc
     2641 ccatccgctt ttaatacata gggagcctta agtgttttca ggtgaacaat tccttcttca
     2701 atattttcct tatgaaatgt catggctttg ccggtcggaa tgccatgcct taccataaat
     2761 tccttggaga aatctttact gccttccagc cgggcgccgg atttacccgg acctataatc
     2821 atcaaacctt ttaattctga atcattttcc aaaaaatctc ttatgccatt aaccagcgga
     2881 gcttcaggcc caactacaac caggttaaca ttatttttct taatgaaggt tgcaacactt
     2941 gcaaagtcat ccggatttaa gggaatattt ttgccaactc cctctgtgcc tgcatttccc
     3001 ggagctataa aaagttgttc acataaatta ctttgggata atttccagga aagtgcgtgt
     3061 tcccgtcctc cggatccgat tacaagaata ttcatcataa atcaattatg atcaaaaata
     3121 taaaaatgtg tgagagcagg gaattaattt gcgtattctg acaaaaactt aatccgcatc
     3181 aatctaattt cttcctctga ataatcctga ttggcgtcat cgtccatagc aacctgaaga
     3241 tcgtcggttt cagcgttgag gaaatattcc atcaaatctt cctgccgttc cgggtcaaga
     3301 acctggttga tataataatc cagattaagc ttggtgccgc tatagataat gttctcaatt
     3361 tcctttaaca cttcttcggg tgtggtgttt ttggtttctg aaatctcatc taaatcaatc
     3421 ttccggtcta tggcctgaat aatataaatt tttaactttg atttatttat cgctgatttt
     3481 accactacat cattagcggt agtaatgtca ttttccttca catacttttg gatggcttcc
     3541 atgaaaacat caccaaactt attgacttta cccataccaa cgccatttat ccttgccaac
     3601 tcttccttcg aggtaggata aattgtagcc atttcatcaa gagaaggatc ctggaaaata
     3661 acataaggtg gaaagccttt ttctttagct actttctttc ttagtgcctt cagaatttca
     3721 aataattcct catcgcatgc ggatgccatt ggagcctggt ccggttcttc ttcttctcca
     3781 aggtcactgt attcatgatc ctttgtaagc ttgagagaga cagggttctc cagaaaatct
     3841 ttccctttat cagacagctt aagcactccg acattgtcga tatctttcaa aataagttca
     3901 tgaagcaagg tctgacgaac cacagacttc caaaattctg ctgaatgttc atcgcctttt
     3961 ccaaaaatat ccagatcatc atggttgtag ctcttcacat attgagactc tacaccacgg
     4021 ataacatcca ctaaatgatc gattccaaat ctttcctttg tttgccgtac cgcatcaagc
     4081 gctgtccaaa caaattcttc gccgtcaaat tcttccttgg gatgaagaca gttatcgcag
     4141 gcgtcgcagt ttccatcaaa actttctcca aaataatgga gaagttgctt tcttcgacaa
     4201 atggaggatt ccgcgtaaga gaccatctcc aaaaggagaa ttgcggcgtt ctcacgctcc
     4261 tgcacggact tgtccttatt gaatttctcc agtttgctga tatcgccggg gctgtaaaac
     4321 ataacacatt ttccttccag tccatctctg cccgctcgtc ccgtttcctg gtaatagcct
     4381 tcaacagatt tgggaacatt atagtgaatc acgaatcgca catccggctt atcaatcccc
     4441 atcccgaaag caattgtagc tacgatcaca tccacttctt cattgagaaa tgcatcctgg
     4501 ttcctttctc tgactgaact atcgagcccg gcatgatatg gagcggcctt gatatcattt
     4561 acatttaaaa gagacgcgat ttcttcaacc ttccttctgc ttaagcaata aataattcct
     4621 gactttcctt tattctcctt gataaatcga atcaattgct ttttggtgtg ctgctttggt
     4681 cttacttcat agtaaagatt ttttctgttg aaagaagact tgaaagtggc tgaagtttca
     4741 atattcaggt tcttgacgat atcgatctga acctttggag ttgccgtagc agtaagagcg
     4801 ataatagcaa ggtcgcctat ctgggtaatg atttgtctta tccttcgata ttccggacgg
     4861 aaatcatgac cccactctga aatacaatgg gcttcgtcta cggcaacaaa agaaatttgc
     4921 gccttcttta gaaaactaac attttcctct ttggtaagag actccggagc cacataaaga
     4981 agcttaacaa aaccatcaag tgtatccttt ttcactttat tcatctcggt ttttgtaaga
     5041 gtcgaattca aaaactgagc atttacaccc aacgcgctta actgatccac ctgattcttc
     5101 atcaatgcaa tcaggggtga tataacaatg gcaactcctt cactcattaa tgcgggcagt
     5161 tggtaacaca ttgatttacc ggcccctgtg ggcattataa cgaaagtatc ctttttgtcg
     5221 agtacatttc ttatgatggg aatttgattt cctctgaatt gcgaatatcc aaagatctcc
     5281 ttgagcttct cttttaggat atcatcttgt tcaatcaaca tcttaattca cagactattt
     5341 tatgcgttaa aatcctttat ctttgccaag taaaatccaa caggtaagga attgaatata
     5401 gcaaaaaata ttaagaaaat agctaaaggt gtacttttaa atgaggctaa ttcaatcaga
     5461 gctatcgcag gttttattga tgataacttt gcagcttgtg tacaggaaat tcttgaaata
     5521 aaaggcaggg taattatcac tggaattggt aagagcgcca ttatagctca aaaaatagta
     5581 gcaactttaa atagcaccgg aactccatct gttttcatgc atgctggcga tgctatccat
     5641 ggcgatcttg gaatcattca acctgaggac atggtgattt gtctgagtaa aagcggcaat
     5701 actccggaaa ttaaagtcct tgtgcctcta ataaaaagaa ggggagttaa attagccgcg
     5761 ctggttagca atacgaattc ttttctggcc aagcaagctg actatattct gaatgcgact
     5821 attgcggaag aagcttgtcc caataatctg gctcctacca caagtaccac cgcgcacatg
     5881 gcggttggtg atgcactcgc cgtctgcctg ttgagactcc ggaatttttc atcggaagat
     5941 tttgccatgc tgcatccggg aggaagcctg ggaaagcaat tatacctcaa agttgaggat
     6001 gtctttcccg ggaatgagct gcctgttgta tctcccgata ctccacttaa ggaggttata
     6061 cttgagattt catcgaagcg gcttggtgcc actgccgtag tgaataagaa acaaaacctg
     6121 ctgggtatta ttaccgatgg ggatatcaga agaatgctgg aaaagcaggc taacattaaa
     6181 tcaacaaagg ctgcggatat tatgacaaaa aatcctaaaa ccgtaagtgt gggagatttt
     6241 gccgtgcatg cacttagcct gatgcagaag aaaaacatca ctcaggttgt tgttgtagac
     6301 aataagaaag ttgtgggctt tgtacatctg cacgatcttt taaaagaagg acttgtataa
     6361 atggctaaac aaaaacccgt agtattaata atggccggag gttcgggaac ccggttttgg
     6421 ccttatagcc gggacaaaca tccaaaacaa tttattgata ttcttggaag cggagaatct
     6481 ctgatccagg ctactttccg aagggcaaaa aaaattacag aaaaaaatcg aatatttgtt
     6541 gcaactaata ttaagtataa gaagctcgtt ttaaagcagc ttccggaaat taccgaatca
     6601 caaatcctgt tggagccacg aaagaagaac acggccccct gcattaccta tgcctctatg
     6661 atattccaaa aaaaattcgg agatgttgct acagttgtaa ctccttcaga ccataccatt
     6721 gtcagagaaa aagaatttat caaagtatta cagcacggta tccggattgc agagaataat
     6781 catggactgg taaccattgg aattaagcca acccgaccgg aaaccggata tggttacatt
     6841 caaacggata aatcaacaaa accggttaag aaagcagtcg ctttcaggga aaaaccttca
     6901 aggaaaaaag cggcagaata tgtcagatcg ggaaactatt tatggaatgc aggaatcttt
     6961 gtttggaaga attcaacaat ccgcaacgaa cttgaaaaat ttcaaccaaa actaattaac
     7021 gattttaagc aagttgaagc gaccttaaca acggggaaca ccaatttgaa ggagttgaaa
     7081 aaggcatttg agaaatgcca ggatatctct attgattacg cggtgatgga aaaggccgga
     7141 aatgtctccg ttattcctgg tgattttggc tggtctgatt taggttcgtg gaacgccgtt
     7201 tttgacctga gcaaaaaaga caaaaatgga aacgttattg cctccaactc tattgttgag
     7261 aaaacaaaaa acagcattat cataggtagt tctaaaaaat tgattgttgt tgaaggactg
     7321 aatgacttcc tggtagctga tcatgaaaat gcaattctta tttgcaggaa agacaatgaa
     7381 aaagaaattc gcgaatttgt aaacagaatt gccaaagaaa agggcaagaa gtttacatag
     7441 tgaatctttg ccggatagct tcgtaaaata ttatacccgc agaaacggaa acattcaaag
     7501 aagcaatttt acccagcatg ggaattttaa tcatcccatc gcacatctta agatattccg
     7561 gtgaaatgcc atcgtcttcc gatcccatta atagtgctac cggatctttc aggttttcat
     7621 taaagtgaag aacttcagct ttttcggtgc accctataat ccgcaacccg gaatctttga
     7681 gaaatgaaat tgtctcttta agattttctg atctgcagat cgggaggtgg tgcaaagctc
     7741 cggcacttgt tttaattgca tctgcacttg tcggagcatt tccccgcgaa ggaaccacaa
     7801 ttgcatgtac gccggcaccc tccgcgcttc tgacgatggc gccgaaattt ctaacatctg
     7861 taattctatc caaaatagca attagcggaa ttttaccatt ttcaaaagtt tgagacaaaa
     7921 tgtggtccag agatgcatac gttataccag acagtaaaca caccgctccc tgatgattct
     7981 ttttcgtgat tcgatccagt ttttcctggg gaacctgcac cattggcaag ttattggccc
     8041 tcgccaattg aataagttct tttattaatt cattcctggc atttttctga atgaaaattc
     8101 tatctacttc cttccctgca tggatagctt caatgatggc cctgatgcca tatatatgat
     8161 cagattcttt caacggataa gagtttcgta tctccctttt ctccacattt caaccgctct
     8221 gtaccattca ttcctataat cggcattcct gattaataca taatgattgt ctgtgaaaat
     8281 gttatttatc ttttcaaagg tgaacttcat tttgggtaaa tcttccgtct tttggtaaat
     8341 ccattcttcc tttgcctggt ttcgaaaaac ttcatccggg ggacccatga taataaaaac
     8401 cattcccata tccgtcttcc atccttcttt gtaattagtg aataaactgt ttgcttcttc
     8461 gacctgatca aaataattac ggattacgga tttcgccatt tccgatgatc gaaagatact
     8521 taaccagtaa ttatccagcg catctttctt atttatggcg gtatttaatt tatcccattc
     8581 agaacgtgta ctaaagtaac gcaaggaatt tattaaatca tttatgtgtg ccaattcggg
     8641 gaaataggaa ttttgaaccc gtatcccaag tccgtgcaat gaagttgtat cagattgaat
     8701 aaaataaagc ccctgacttg ttaaagatat tgaattgcca gctccaacac taaaaagtga
     8761 atcgatagat aatgaagcgt ttcctgatga ctccgtcatc ataggaggat cagccggctc
     8821 aaaatcaatt tgatagtggt atgcaaatag taaagagtct ttttgggttg aaactatatt
     8881 aaccggattc tcgtttgaaa tccaatcgcg aattaccggc aaattttcgg aatcgtaaat
     8941 caggatagaa gattttcgtg tattcgataa cggtacccaa taatagtaat gtttttgatt
     9001 gtgacggttt gcaaaatgaa gcacatagat tttattttga agatttggat ttaactttat
     9061 tctgaaatta aatccccccc ttgttcgctt aataatatag tctagaattt gaaccgtatc
     9121 agaagtgtta ccaattttgt cactgtagga gttgacatat tcttcaaaaa catagtaatc
     9181 cgtcatctca taaacatcct ttctcatttt tatatgaagc agcaattctg aagattcacc
     9241 agaataaatt agcctgcatt tgatatccat ctccgaattt ctattatata gatagctgag
     9301 attctggtct gcaagactct ctgccgtacc aggatccatt ttgaagaata gggaaataca
     9361 aagtaagtat gacaacatca ttatttctgc aaattacttc aatatcgatt ttaattctag
     9421 caggaggtta attttgcatt aaatttgagt gaaattttca cagggtgggt atattcacaa
     9481 ccgaaattac ttcggataaa attttgtcgg acaacccgat ccatcagcgg ttgctgaaag
     9541 cctaccgcct ggccgctcct tttgttaatg gtgatgtact tgaacttggg gtgggagagg
     9601 gaagggggct taaacttctt gcaccgtccg caggatcata tacggcatta gataaactta
     9661 agagttcaat tgaaattcta agtgcccaat accccgaagt tagatttatt cactcgagta
     9721 ttccacctgt taaagacctt gaatcaaatt cgtttgattt ggtaattagt tttcaggtaa
     9781 ttgaacatat taaaaatgac cggctctttc ttgaagaaat tggccggctg ttgagacctg
     9841 gtgggaaatg tctgataagt acgcctaaca tcaaaaaaac cctgacaaga aacccctggc
     9901 acataaggga atatacgcgt gaagaattat atgctttagc taaaccaatt tttggacaaa
     9961 ttgatattaa gggaattaca ggatctgaaa aggcatggaa atactatcaa caaaataagg
    10021 aatccgtggc aagaattatg aaatatgaca ttttcgactt acaacaccgg cttccggctc
    10081 ctttacttcg aatcccgtat gaaattttaa accggattaa ccgcaataaa ttgcaggcaa
    10141 aagataatga actggtttct cagatttctg aggcagatta tatgttgtca g
//
LOCUS       DASWHX010000222         5740 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_3576021, whole genome shotgun sequence.
ACCESSION   DASWHX010000222 DASWHX010000000
VERSION     DASWHX010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 5740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5740
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3576021"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(312..1412)
                     /locus_tag="VGA21_14460"
     CDS             complement(312..1412)
                     /locus_tag="VGA21_14460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020530333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEX9651762.1"
                     /translation="MSAFIHQLDNWPDFTWRLNELINLLSEGRNLQGRIFGRMESIGF
                     DLRKEATLETLILDVLKSTEIEGEILNPNQVRSSIARRLGMEIAGSVESDRNVDGMVE
                     MMIDATQNSFKPLTKERLFDWHAALFPTGRSGMYKITVADWRTDSTGPMQVVSGAMGK
                     EKVHFQAPHSSLIETEMSRLLTWFNTNNELDLVLKAAVAHLWFVTIHPFDDGNGRITR
                     ALTDMLLAQSDRSTQRFYSMSAQIRLERKQYYEILEKTQTGNLDITDWIKWFLSCLIN
                     ALKSTDAILNRVLFKADFWNRHAKTVMNERQKKLLSKILDGFEGKLTSSKWAKIAKCS
                     KDTAIRDINDLISKNILQKEEAGGRSTKYELK"
     gene            complement(1723..1980)
                     /locus_tag="VGA21_14465"
     CDS             complement(1723..1980)
                     /locus_tag="VGA21_14465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651763.1"
                     /translation="MYTYLSPDEIIWLAVLPVTTVIALAGIWFGKGWGIILAVIVFAA
                     ILLLDVYYKVWPHAILATVGFALLAFFCWQSRQYFMIMKES"
     gene            complement(2546..4114)
                     /locus_tag="VGA21_14470"
     CDS             complement(2546..4114)
                     /locus_tag="VGA21_14470"
                     /inference="COORDINATES: protein motif:HMM:NF036516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M61 family peptidase"
                     /protein_id="HEX9651764.1"
                     /translation="MVKHQAKAKLFLFCILTVWQLPAWAQVKPVIEYTVSVPQPATHK
                     YHVELRTAGWNQDTVTFKMPKWMPGYYQIMDYAKSVENIFVRDEKGKDLPFQKVNDNT
                     LRISGIRNKSLILSYDVMTARQFVANSYVDSEHAYIIPAGTFFYIDGFLHLPLNVEIV
                     TTPWNNIATGLEPVTGKPNQFTAPDFDILYDCPILIGNLEELPPFKVNGIEHRFIGYK
                     PGMFDRKDFIDKLKRITEAAVAVIGDIPYNQYTYIAIGPGRGGIEHLNNTTVSFDGSS
                     LSNPDAMNRMMNFLAHEYFHHYNVKRIRPFELGPFDYEKGSRTNLLWVSEGLSVYYEY
                     LIVRRAGLADERTLLNNLEGNINSYENNPGRFHQSLAQASYNTWKDGPFGTQGEERDK
                     AISYYDKGPIAGMLLDFAIRHTTQNEKSLDDVMRLLYWKYYKAKSRGFTDAEFQQTCE
                     EVAGTSLTQIFEYVYTTRELDYTKYLSFAGLKMEELPNTDVDKKQTRKFTLSRIENPD
                     ALQKSILKSWQGEK"
     gene            complement(4374..5291)
                     /locus_tag="VGA21_14475"
     CDS             complement(4374..5291)
                     /locus_tag="VGA21_14475"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX9651765.1"
                     /translation="MKKLKILSIFLFLGQLSVPVLGQERLPVIDMHMHIGAPLDLPAG
                     GPAPCLPQPCVRQGHASANSADNLKRTLEVMDRYNIVKGFLSDLDLSELREWAKAAPD
                     QFIISPFILDPSKAASPDDLRKELETKRIAGIGEIGSQLIGIAPNDPRLEPYFALAEE
                     FDVPVLIHTEGIGPPLPSFRSAAGSPLLLEEVMVRHPKLRIFVENSGFPYLDEMIAMM
                     YQYPQLYGDVSTMTWIIPRSAFYDYLQRLIKAGLGKKLMFGSDQMLWPEMISKGIEAI
                     EEADFLTSEQKRDILYNNAVRFLQLEKKK"
     gene            complement(5476..5733)
                     /locus_tag="VGA21_14480"
     CDS             complement(5476..5733)
                     /locus_tag="VGA21_14480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014771086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HEX9651766.1"
                     /translation="MKLVFVDESWEDYLYWQKTDKKILKRINSLIKDITRNPFDGIGK
                     PEPLKYKYSGFWSRRIDGEHRLIYQVKEDEILIAKCRFHYD"
BASE COUNT         1570 a         1217 c         1100 g         1853 t
ORIGIN      
        1 caaacttatc catcatttgg agcggaacta cacctatgtt ccttctaaat tgtatgactc
       61 cctggtaagg atgaatcaga attacaaccg gattgttcac aggaatttca agcaggaata
      121 ttctgcaaat gtggcctgaa atgtgacatg ttttactttt ctcaggaatt agttttttga
      181 ttttgagtta aacgtgccgg caaggagtcc ggttggcaat gctatcaaag gtgcaagaaa
      241 agttgaagcg ggcttcaacc cttcgcatac aactggaacc cctggttata ccgcatattg
      301 cctgctggca tttatttcaa ttcatatttt gtacttctac caccagcttc ttccttctgt
      361 aatatgtttt tgcttattaa atcgttaatg tctcgtattg cagtatcctt tgagcatttt
      421 gcaatctttg cccattttga agatgtcaac ttaccttcaa atccatccag aattttactc
      481 aacaacttct tttgtctttc gttcattact gttttggcat gcctgttcca aaaatcagcc
      541 ttaaataaaa cccggttcaa aatggcatcg gtcgatttaa gtgcatttat caagcagctt
      601 aagaaccatt taatccagtc ggttatgtca agatttcctg tttgtgtttt ttcaagaatt
      661 tcatagtatt gctttctttc aagtctgatt tgggctgaca tactataaaa acgctgtgta
      721 cttctgtctg attgtgctaa taacatgtca gtcaaggctc tggttatacg accattccca
      781 tcgtcaaatg gatgtatagt tacaaaccat aaatgagcaa cagcggcttt tagtaccaaa
      841 tcaagttcat tatttgtatt aaaccatgtt aataatcgac tcatttcagt ttctatcaaa
      901 gatgaatggg gcgcctgaaa atgaaccttt tctttgccca tggctcccga tacgacttgc
      961 atagggcctg tagaatcagt tctccaatct gcaactgtta ttttatacat tccgcttctt
     1021 cctgttggga agagcgcagc atgccaatcg aaaagtcttt cttttgttaa tggtttgaaa
     1081 ctattctgag ttgcgtcaat catcatttca accattccat ccacattcct gtcggactca
     1141 actgaacctg cgatttccat tcctaatctt cttgcaattg aagaacggac ttggttggga
     1201 ttcaatattt caccttcaat ttctgttgat tttaaaacgt caaggattaa tgtttcaagc
     1261 gttgcttcct ttcttaaatc gaaccctatt gattccattc ttccaaatat gcgtccttgc
     1321 aaatttctcc cttcacttaa taagtttatg agctcattaa gtctccaggt aaaatctggc
     1381 caattatcta attggtgtat aaacgcactc attcgctgca tattatgcaa cgaaaatacg
     1441 gcttattcgt cgcattccaa aatttatcgt tgcgcatttt gcgttgattg caccagttaa
     1501 tcattgtacg tgcgttatta ccttcatttt aaaatacttg atgtcttttt atgtttctaa
     1561 ctaacgtttt tgggcttggc gaagtggcgg catttcagca ccaaagttta ttagatgcac
     1621 taacttttgt ttaattataa atgttataga tgcaccaaac caaatataac caacggcaga
     1681 caggataaaa attttgatgt tatccgtaac atgatttggt tctcagcttt ccttcattat
     1741 cataaaatac tgcctagact gccagcaaaa aaatgcaagc agagcaaacc caacggtggc
     1801 aagaatagca tgtggccata ctttataata cacatcaagc aataaaatag cagcaaatac
     1861 tattacggca aggataattc cccaaccttt gccaaaccag atacctgcca gggcaataac
     1921 tgttgtaaca ggcagtacag cgagccatat tatttcatct ggggataaat aggtatacat
     1981 ctgcgaaagt tgctctgcat ttatttgcag tttaaaaatt ccagcaatgt tggataaggc
     2041 aagtgaatga taacatgctc aatcctattt ttcttttttt catttccggt aagtttttct
     2101 gtctgttaat atttattatg tggtgttaat cagtcaagtt gattggaact atcactcaga
     2161 ggtctggaag acactgccca ggtagctttc tgtatgagtc tcaatccact ttgtcaagtt
     2221 actctgtctt tagagttgcc gctaacggtt ttgcttttta tttttttgcc caatcataga
     2281 catcttttga taaggactgg gaactttttt gcaggttgaa tatttagaat gttggtgttg
     2341 gatttgagtt ggattaggaa atccaactat tcattaggca cggataaagg atattcgcac
     2401 ccactgagag gcgcaaacta aattgaaaaa gcgtttttca cttgtgaagg tgaattataa
     2461 acatcagagt cctctagcga gagactctga tggggataaa aaacacaata cttcttcttt
     2521 cggatccacc ggtcaaaagg caaaatcatt tttcgccttg ccacgatttt aatatggact
     2581 tctgcaacgc atccggattt tcgatgcgac ttaatgtgaa ctttcttgtt tgcttcttat
     2641 caacatccgt gttgggtaat tcttccattt tcaaccccgc aaaacttaaa tactttgtgt
     2701 aatcaagttc acgggtggtg tatacatatt caaatatctg tgtcaacgag gttccggcca
     2761 cttcttcgca tgtttgctga aattcagcat cggtaaatcc acggcttttt gctttgtagt
     2821 atttccaata cagcagtcgc atcacatcgt ccagcgactt ttcattttgt gtcgtatggc
     2881 ggatagcaaa atccagaagc attcctgcaa tcgggccctt gtcataatag gagatagcct
     2941 tatcgcgctc ctcaccttgt gttccaaagg ggccgtcctt ccatgtatta taactagcct
     3001 gtgccaggga ttgatggaac cggcccgggt tattctcgta agagttaata ttcccttcca
     3061 ggttattaag caatgtgcgt tcatcagcga gtccggctct tctcacgatt aaatactcgt
     3121 agtaaaccga taacccttcg cttacccaaa gcaggttggt tctgcttcct ttttcatagt
     3181 cgaagggtcc gagttcaaac ggccggatac gctttacgtt gtaatgatga aaatactcgt
     3241 gagcaagaaa attcatcata cgattcatag catcagggtt gctgagactg ctgccatcaa
     3301 aactgactgt ggtgttgttc agatgctcaa ttcctcctct gcccgggcca atagcgatgt
     3361 aggtatattg gttgtacggg atatcgccaa taactgccac agccgcttct gtaattctct
     3421 ttagcttgtc aataaaatct tttctatcaa acatgcccgg cttgtagccg ataaaccgat
     3481 gttcaattcc atttacttta aaaggtggca gttcctcaag atttcctatc aggatagggc
     3541 aatcataaag gatatcaaaa tcaggggctg tgaactggtt gggttttcct gttaccggtt
     3601 ccaatccggt tgcaatatta ttccatggtg ttgttacaat ctccacattt aaaggcaggt
     3661 gaaggaaccc atctatatag aagaaagtgc ctgcgggaat tatataggca tgttcactat
     3721 cgacataact gttggccaca aattgcctgg ctgtcatcac atcataactt aaaatgagag
     3781 acttgtttct gattcctgag atgcgcaatg tgttatcgtt cactttttga aaaggtaaat
     3841 ctttcccttt ctcatctctc acgaaaatat tttcaactga tttcgcatag tccataatct
     3901 gataatagcc tggcatccat ttaggcattt tgaaagtcac tgtatcctgg ttccagcctg
     3961 cggtacgaag ttctacatga tacttgtgtg tggctggttg cggcactgag acagtatatt
     4021 ctatcacggg ctttacctgc gcccatgcag gcaattgcca caccgtgaga atgcagaata
     4081 agaagagttt tgcttttgcc tgatgcttca ccatttgtgt ctgggttgta tttgttgtta
     4141 acttctcgta ttcgttaggg ttcgctgtta aggtatgaaa ttaatcaaat gctttagtat
     4201 gtaacggttt cgggctttac gttggtggcg gcttagaagt acaacacttt cgccttgcac
     4261 caaagttaat taaatgcact actgtttgat taagcattgc acccgccatt tcgtttaagc
     4321 ccttgcacag ttatagtttt taataaattc aggcataaat aattttcctc tccctatttc
     4381 ttcttttcca gctgaagaaa cctcacggca ttgttataca ggatatctct tttttgttcg
     4441 cttgtcaaaa aatcagcttc ttcgatggct tcgattcctt tgctaatcat ttccggccaa
     4501 agcatctggt ctgagccgaa cattaacttt ttgcccagtc ctgccttaat gagtcgttgc
     4561 agatagtcgt aaaaagcgct tcgcggaatg atccaggtca ttgtagatac atctccataa
     4621 agttgaggat actggtacat catggcaatc atttcgtcaa gataaggaaa gcccgaattt
     4681 tccacgaaga tcctgagctt tgggtggcgg accataacct cttcaagcag gagaggacta
     4741 cccgcggcag atcgaaaact gggcagaggc ggtccgatgc cttccgtatg aattaaaaca
     4801 ggaacatcaa attcttctgc taatgcaaaa taaggctcaa ggcgcggatc gtttggggca
     4861 ataccaatga gctgcgaccc gatctcacca atgccggcaa tacgtttcgt ctcaagctcc
     4921 tttctgaggt catccggtga agccgccttt gacggatcaa gaataaaggg agatataata
     4981 aactgatcgg gtgccgcctt ggcccattcc cgtagctcac tcaggtcaag gtcactgaga
     5041 aaacctttca caatgttgta gcgatccatt acttccagag tccttttaag attatcagct
     5101 gaattggcag atgcatgtcc ctgcctgacg cagggctgtg gcagacaagg ggcagggccg
     5161 cctgcaggta aatccaatgg agcaccgatg tgcatatgca tatcgataac aggcagtcgt
     5221 tcctgaccaa gcacgggaac agacaactga cccaaaaaca gaaagattga aagaatttta
     5281 agttttttca tattagatga tttttaatag ctcaaggcta atgaatatta attgtgcaca
     5341 acggttggcg cataaaaaat gtagggcttt tggaacgcta atttgtcccc gtgaataaag
     5401 tttgttgaaa gaaataaaac tctataaaaa cctagtcaga cctatatttt ttatgctgct
     5461 gttgccacac gttttttaat cataatgaaa cctacatttt gcaattaata tttcatcctc
     5521 tttcacttga tatattaatc gatgttcgcc atctattctc ctagaccaaa agccggaata
     5581 tttgtatttt aaaggctctg gtttacctat tccatcaaat ggattccttg taatgtcctt
     5641 tatcaaggaa tttattcttt tgagtatttt tttgtccgtc ttctgccaat acaaatagtc
     5701 ttcccaagat tcatcaacaa acactaactt cattcctcaa
//
LOCUS       DASWHX010000223         3903 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_3578527, whole genome shotgun sequence.
ACCESSION   DASWHX010000223 DASWHX010000000
VERSION     DASWHX010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 3903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3903
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3578527"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(251..757)
                     /locus_tag="VGA21_14485"
     CDS             complement(251..757)
                     /locus_tag="VGA21_14485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018479859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2480 family protein"
                     /protein_id="HEX9651767.1"
                     /translation="MDEIINKVARSNLKVIDLAEYRDGSERLFFDLKDLLFQGQILRE
                     NNFRDFIANHDWASYKGKNIAIGCSIDAIIPAWAYMLLVTKMQPFAGKIIIGNLEMLE
                     NEIFRDAISSLPLNEFRNAQIIIKGCGEVPVPDFAYAEITRILRPVAASIMYGEPCST
                     VPIYKKTK"
     gene            complement(762..1451)
                     /gene="tsaB"
                     /locus_tag="VGA21_14490"
     CDS             complement(762..1451)
                     /gene="tsaB"
                     /locus_tag="VGA21_14490"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEX9651768.1"
                     /translation="MALILSLETATKVCSVALHDKGVLLAEMNVMEEYSHFEKLPTLI
                     EQVLKESNRKFSDLKCIAVSMGPGSYTGLRIGVSMAKGIAFSLNIPIVSVNTLKGLAV
                     SAMEKAGEKELLCPMIDARRSEVYTMIVNHHLEEIWPTQALIIDKHSFNEVIENHNLL
                     VFGDGSNKFISLFESFTKIRFNELSPSAKQIGELGWKKFQNAEFENTAYFEPYYLKNF
                     RPGIGKKKLTL"
     gene            complement(1452..3125)
                     /locus_tag="VGA21_14495"
     CDS             complement(1452..3125)
                     /locus_tag="VGA21_14495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014799406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEX9651769.1"
                     /translation="MPIKAFDKINRKWIVLIGSLSVILILAFSGPGEKYFEVARNLDI
                     FASLFKEVNGYYVDEVDPKNLVAIGITAMTESLDPYTVYIPEEEAADFRIITTGKYAG
                     IGIMVARINGQIAVTMVEEKYPAFKAGIKIGDRIIEVDGHSLATTMHEEISRLLKGEI
                     DKEVKVKVERYGEPQPIEFRLKRQNIKIANVHYSGMLDSQTGYIRLSDFTNDAGKEVI
                     AALDSLKKLGASSLILDLRGNLGGLLNEAIAVCNVFIEKGKEVVSTRGQVPDWNRSHA
                     TLGAPVDKDIPIAILIDNRSASAAEIVAGVIQDYDRGILVGNKSFGKGLVQTTRSLPY
                     NAQLKITTAKYYIPSGRCIQAIDYANRNEEGFVDKIPDSLKVAFKTANGRTVYDGGGI
                     DPDFETGKQILAAITNELLNKGLIFDYATEYYYHHTQITPLDKFELTDKDYNDFVKWV
                     QSKDFEYNTRVEMELKHIEETASNEKYYGALKERISSLRKELEERKRNDFDIFKEEIK
                     NLLAMEIVSRYYFQRGQLRYMVRDDAVIKRATHLLQDQTQYNKALQARK"
     gene            complement(3115..3525)
                     /gene="rnpA"
                     /locus_tag="VGA21_14500"
     CDS             complement(3115..3525)
                     /gene="rnpA"
                     /locus_tag="VGA21_14500"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEX9651770.1"
                     /translation="MTTHVMATKNPKYPNKFKKNEKLSNKKEIEALFASATAIKVTPL
                     KILFLVREDLDLPQVLISIPKRNFRKASARNLLKRRIREAYRINKHLLSPGPGQNPMI
                     AYIYLATENLPFKTIENSVRTSLKKLNEKITHAN"
     gene            complement(3547..3705)
                     /gene="rpmH"
                     /locus_tag="VGA21_14505"
     CDS             complement(3547..3705)
                     /gene="rpmH"
                     /locus_tag="VGA21_14505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011583506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEX9651771.1"
                     /translation="MKRTFQPSKRKRKNKHGFRSKMETANGRRVLASRRAKGRKKLSV
                     SDERRHKG"
BASE COUNT         1077 a          878 c          633 g         1315 t
ORIGIN      
        1 agccttttct cttttaaact catctctttt gactcgtcct ttagagcggt tcgctatcat
       61 tccaattgtt caaagaacgt ccgcattctt gtctccaaaa atacttcgac aacattttct
      121 atgttgttac ttttttgccc cgccttctga tcggtttccc ctctttcccc ctcaaaacgg
      181 attgcaaagg taggtggaag ttaattagta atcaaatctc ttttgtgaaa taatttagag
      241 agaattgaat ttatttggtc tttttataaa tcgggactgt actgcaaggc tcaccataca
      301 ttatgctggc tgcaaccggt cttaaaatcc ttgtgatttc agcatatgca aaatccggaa
      361 cgggaacctc accacatcct ttaataataa tttgagcgtt tctaaattca tttaatggaa
      421 gcgaagaaat tgcgtctcga aagatttcat tttcaagcat ttccaaattg ccaataatta
      481 ttttgcccgc aaaaggctgc attttagtaa ccaataacat ataagcccaa gccggaatga
      541 ttgcatcaat gctacaaccg atggctatat ttttcccttt atatgatgcc cagtcatgat
      601 tagcaataaa atccctgaaa ttattctctc taaggatctg tccctgaaac aatagatctt
      661 taagatcgaa gaataatctt tcagaaccat cacggtattc cgctaaatcg ataactttta
      721 gattactcct ggctactttg tttattattt catccatctc attacaaagt taacttcttt
      781 ttgccaatac ctggcctgaa gttcttaagg taataaggct caaaatatgc cgtattctcg
      841 aattctgcat tttgaaattt tttccagccc aattcaccta tctgtttagc tgaaggggac
      901 agttcattaa atcgaatttt cgtaaatgac tcaaataatg atatgaattt gttagaacca
      961 tcgccaaaaa ctaaaaggtt atgattttca attacttcgt tgaaagaatg cttatcaata
     1021 atcaaagcct gggtaggcca aatctcttcc aaatgatgat tcacaatcat tgtgtaaacc
     1081 tccgaccttc ttgcatcgat cattggacaa agcaattcct tctcacccgc tttttccata
     1141 gcagaaactg ccagaccttt taatgtattc actgacacaa ttggtatatt caatgaaaag
     1201 gcaatgccct tagccatact aactccaatt cttaaccctg tataggatcc aggacccatc
     1261 gaaactgcaa tacatttcag gtcagaaaat tttctgttcg attcctttaa aacttgttct
     1321 atcagtgtgg gtagtttctc aaaatgggag tactcttcca ttacattcat ttcagccaac
     1381 agcactcctt tgtcatgcaa ggcaactgag caaacttttg ttgctgtttc caggctgaga
     1441 atcagggcca tttattttct tgcctgaagg gccttgttgt attgagtttg atcctgcaat
     1501 aagtgggttg ctctttttat tactgcatca tccctgacca tataccgaag ttgacccctc
     1561 tgaaagtaat atcttgaaac tatttccatt gccaacaaat tcttaatttc ttccttaaag
     1621 atatcaaagt cgtttctttt tctttcctcc aactcctttc tcaagctgct gatcctttcc
     1681 tttaaagctc cgtaatattt ttcattcgac gccgtctctt caatatgttt caactccatt
     1741 tcaaccctcg tattgtactc aaaatccttt gattgcaccc attttacgaa gtcattgtaa
     1801 tctttgtcag tcagttcaaa cttatccaga ggggttattt gcgtatgatg gtaataatac
     1861 tccgttgcat aatcaaatat taaacccttg tttagtaatt cattcgtaat tgctgcaaga
     1921 atttgtttcc cagtttcaaa atcaggatct attcctccgc catcataaac tgttctgcca
     1981 tttgccgttt tgaatgccac cttcaatgaa tcgggtattt tatccacaaa tccttcttca
     2041 tttctattag cataatcaat agcttgtatg catctgccac tgggtatata atatttggcg
     2101 gttgtaatct ttaattgagc attataggga agacttcgag ttgtctgaac caatcctttt
     2161 ccaaatgatt tgttacccac caatatacct cgatcgtaat cctgaattac gccagcaaca
     2221 atctcagcag cagatgcgga cctattatct atgaggatgg caattggtat gtccttgtca
     2281 accggcgctc ccaaagttgc atgactccgg ttccaatccg gcacctgccc ccgcgtactt
     2341 actacttctt tccctttctc aatgaataca ttacaaacag caatcgcttc atttagcagg
     2401 cctcccaaat tccctcttaa atcaagaatt aaagatgaag cgcctaactt ttttaaagaa
     2461 tccaaagctg ctatcacttc cttgccagca tcgttcgtaa aatccgagag cctaatgtat
     2521 cctgtttgtg agtccagcat gccagaatag tgaacattag ctatcttaat attctgtcgc
     2581 tttaatctga actcaatggg ttgcggctct ccatacctct ccactttaac ctttacttct
     2641 ttgtcaattt cacctttcaa aagccggcta atttcctcgt gcatggttgt cgccaaggaa
     2701 tgcccatcaa cttcaattat tctatctcca attttaatcc ccgctttgaa tgcaggatat
     2761 ttttcctcaa ccatggtgac agcaatttga ccatttatcc gggcaaccat tattcctatc
     2821 ccagcatact ttccggttgt tataattcga aaatcagctg cctcttcttc aggtatataa
     2881 accgtatagg gatccagaga ttcagtcatg gcagttattc cgattgcaac aaggtttttt
     2941 ggatccactt catcaacata atagccgttg acctccttaa aaagcgaggc aaaaatgtcc
     3001 aggttgcggg cgacttcaaa gtatttctca ccgggaccgc taaaagccag aataaggata
     3061 actgataaag aacctatcaa tactatccat tttctgttta tcttatcaaa tgctttaatt
     3121 ggcatgagtt attttttcat ttagcttctt caaggaggta cgcacggaat tttctatcgt
     3181 cttaaatggc aagttctctg ttgctaagta aatataagca atcattggat tttgtcccgg
     3241 cccaggtgat aataagtgct tattgatcct ataagcttct ctaattcttc tcttgagcaa
     3301 atttctggcc gaagcttttc gaaaattcct ctttggaatt gaaattaaga cctgaggaag
     3361 gtccaaatct tccctaacca ggaaaagtat ttttagagga gttactttaa ttgcggttgc
     3421 gctggcgaac aatgcttcaa tctccttctt gtttgaaagc ttttcattct ttttaaattt
     3481 atttggatat tttgggtttt tggtagccat aacatgggtc gtcatggcgg cggatataaa
     3541 ttgttattat cctttatgcc gtctttcgtc agaaacagat agcttctttc ttcctttcgc
     3601 tcttcggcta gccagaactc ttctgccatt tgcagtctcc atcttagacc tgaagccatg
     3661 cttgttcttt cgtttccttt ttgaaggctg aaatgttctc ttcatctctt tttcatcaat
     3721 taatgggctg caaagataac cagtctttaa tgaaataaca aagcctaagg agatgctcaa
     3781 gactatttgg ggggcacaac taatgataaa tgggcttgaa atcaaaaaag agctccggga
     3841 atagcttccc ggagctgtcc taatcatccc cacacacaaa ccacacatag gcggtatttt
     3901 tag
//
LOCUS       DASWHX010000224         8857 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_1774094, whole genome shotgun sequence.
ACCESSION   DASWHX010000224 DASWHX010000000
VERSION     DASWHX010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 8857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8857
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1774094"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2394)
                     /locus_tag="VGA21_14510"
     CDS             complement(<1..2394)
                     /locus_tag="VGA21_14510"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX9651772.1"
                     /translation="MKNSETITVTKSKEGKQINGKTDLKLAFIDDIPFEIKPGETILS
                     FLNRHIGKGKVPTLCDAPNLKPYGACRVCSVDVALTHNGNRKVQASCHTPISENTYVY
                     PNSERISSLRKNILELILTDYPEEKFKTDSPINNELKAVSGMLGIEPAQIRYPKGRNH
                     FDRKPDKSHPYMISDLSGCINCYRCVRACDEVQGQFVLSTAGRGFDTKIIMGMDTTFA
                     ESECVSCGACSQNCPTNAISDVFKSFALEADKTTRTVCTYCGVGCNLEVSSKGQEIVS
                     IRAPWDAEVNQGHTCLKGRYAFKFYNHPDRLRSPMIKRSGKFEEVSWDEAYDFITGKL
                     TAIRDEFGPDSIGGISCARATNEENYLMQKFIRAVIGTNNIDGCARVCHSPTALGMQR
                     SFGTGAATNSIEDINHTTCMLVIGANPTVAHPVTGAKIKQKAMKGIPLIIIDPVEIEL
                     VKYAKHFLQVRPGTNVAVLNMMLYYIINENLVDEEFVAKRCEGFVEFKSDILRLNMDE
                     LSKITGVDKEEVRAAAITYAAAELAMEFHGLGVTEHSQGTFAIQLIANLAMITGNIGK
                     KGVGVNPLRGQNNVQGMADMGVQPHQGAGYLDVTQPEIHSMYTTFYKRPVSDKVGLKI
                     PQMFDAAIESNLKALWIIGEDVVQTDPNTQHVKKALDNLELLVVQELFMTETAKRATV
                     ILPATSFLEKNGTFTNGERRIQRVNEVVEPLAGTKPDGQIIVEIMNRMGYEQPDYHPR
                     TALEEISRVVPFFKGVKWDELGDNGKQWPVDENGKDSKIIHIDTFKRGKGKFHYIDFR
                     "
     gene            complement(2399..4060)
                     /locus_tag="VGA21_14515"
     CDS             complement(2399..4060)
                     /locus_tag="VGA21_14515"
                     /inference="COORDINATES: protein motif:HMM:NF013662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HEX9651773.1"
                     /translation="MSDNVSALSGRKGLDRNLFEELVKEGKKNGTPEPEVLRQIAKDF
                     LIGDSITYGASTFYDFLKPENKQIKAYICNGSVCMLAGTQENLKTKLLEHLGEEEIGH
                     MCCLGRCHENGSFYYRGENYSGKSDSEIDEIMQGSAGKNKDQYFVGTNMAEPILTAPF
                     PDLQEFYARLIKAFSRDPEKLLQEIKDSEVRGRGGAGFKMSFKLESCKNTPSDQKFIV
                     CNADEGDPGAYSDRYLMEERPHLVLFGMMVNGFITGASRGVLYIRGEYPESVSAVNLA
                     IAELEELGLLGDNIKGSGFDFRFKVIEGAGAYVCGEETALLSSIEGQPPHVRVRPPYP
                     TQEGLFNKPTVVNNVETLACIPYIINNSGKVYAGIGTGKSSGSKLVCLDSFFNRPGMY
                     EVAMGTTLKEVVNELGQGFKQPIKALHIGGPLGGIVPVHKIPDLTLDFESFAENGFLL
                     GHAGVVSIPENFPMIKYLEHLFEFTAAESCGKCVPCSIGSVRGMEMLVKAQHEDYQID
                     RKLMDDLLETLEVGSLCALGGGLPLGIKNALQYFENELKPYFIKN"
     gene            complement(4193..4663)
                     /locus_tag="VGA21_14520"
     CDS             complement(4193..4663)
                     /locus_tag="VGA21_14520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009283589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX9651774.1"
                     /translation="MQIAKNKVAAIHYTLTDNDGNTIDSSQGRDPLLFIQGVGNLIPG
                     MEEGLEGRARGDKFKIKVRPEKGYGEINPKLVQDVPRKSFGDQEVQPGMQFQTNQGQV
                     LTVKEVKTEVVTVDANHPLAGRELNFDVEVMEVREATKEELDHGHVHGKGGHHH"
     gene            4843..5034
                     /locus_tag="VGA21_14525"
     CDS             4843..5034
                     /locus_tag="VGA21_14525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651775.1"
                     /translation="MKRMFTFLLAIICCQAYGTEVIGNYKTEVIVNLDRSVLVKEIIE
                     VTVEGINIRRGIYRDIINV"
     gene            5056..6747
                     /locus_tag="VGA21_14530"
     CDS             5056..6747
                     /locus_tag="VGA21_14530"
                     /inference="COORDINATES: protein motif:HMM:NF044955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HEX9651776.1"
                     /translation="MRYPVSVVSVMKDGVPEPYSVDEFDQFVRVKIGNPDILLENRVH
                     KYEITYTMPEQVRFFDNHDEIYWNAIGTGWDFEIQYAEARVFLPDGAIITEYAGYTGY
                     FQDTSCECEIVIVNENELEIKTTGSLYPNQGLTVAAGFSKGVIPPPTLSDKDRYLVKP
                     DLIYTLGAIGLGIVLIYFMVSWYLVGKDPEGGTIVPLFYSPTGISPAASRSLTKMGFD
                     NKSFTASVVNLAVKGIIKIEKIKDGYKLHKLVENPADLPMEEKSIFDKLLKHQNSFSI
                     GDKYSSTLNKALSNHKESLIKELSKTYFNTNQKWLIPGVLISIVTIFVCFGATLNYDE
                     NTFIGLIPIGMISIIFFPILVAIFKKLSSLGSLSQALTMLGGLAFLGFIGYNYFGILD
                     AIDLLGSIDVYVVYFFGILLISLLTLNFLFSYLIKAPTVSGRKLMDKVDGLKMYLNVA
                     EKDRLEQMHAPDQTIEHFEKLLPYAIALDQEKHWSDRFETLLASASEGGYQPTWYTGG
                     TIRAITFSTLTRDIGRGFNSSVSSASTPPQSSSSSGSSGGGSSGGGGGGGGGGGW"
     gene            6772..6993
                     /locus_tag="VGA21_14535"
     CDS             6772..6993
                     /locus_tag="VGA21_14535"
                     /inference="COORDINATES: protein motif:HMM:NF022823.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3185 family protein"
                     /protein_id="HEX9651777.1"
                     /translation="MKLPKLLVGIVLLVIGIFVIYWAYNHSPEAGLGDRIVDALEGEK
                     VLSKTTYYTMLVSGTLLIILGITRTVKSL"
     gene            complement(7002..7964)
                     /locus_tag="VGA21_14540"
     CDS             complement(7002..7964)
                     /locus_tag="VGA21_14540"
                     /inference="COORDINATES: protein motif:HMM:NF024579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651778.1"
                     /translation="MKYFQFFFFLIAINACSVGNDQNKIDEEVDGSEKKPAAYSLDGN
                     ALYPPELAPETLARLTANLDSARQNYEADSTDEMNIIWYGRRLAYLSLYPEAIEVFSK
                     GLELHPKSFKLYRHRGHRYISTRQFDKAIDDLEKAAWFIAGEELMIEPDGAPNKLNIP
                     LSTTQFNVYYHLALAHYLKRDYRKAINTYLECMKVSDNNDLLVATTDWLYMSYRKIRR
                     DIDAKKLLDAISADMEIIENESYLNRLLMYKGTKTPEELLQVAGDQEDNGVTIATQGY
                     GVGNWYLINGDREKAKEIFTQVVSGTSWSAFGFIAAEAELRRMK"
     gene            complement(7975..8529)
                     /locus_tag="VGA21_14545"
     CDS             complement(7975..8529)
                     /locus_tag="VGA21_14545"
                     /inference="COORDINATES: protein motif:HMM:NF022588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HEX9651779.1"
                     /translation="MLKKIFIILAIIAGLVIIYNIYLLATTKSHSPAEVVEYTNESLS
                     IAITYSRPYKKGRLIFGEGEKALVPFGEKWRTGANEATQISFNQNIYLEDSLLKKGIY
                     SLYTIPGKTEWVLAINSKTNYWGRTLVGNPFEEELDVVRVKGRVSETDTEIEQFTINF
                     TESDSLLQLNMLWDRSKVTFNIKN"
     gene            8631..>8857
                     /locus_tag="VGA21_14550"
     CDS             8631..>8857
                     /locus_tag="VGA21_14550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651780.1"
                     /translation="MKYLYQKQFQNALSACALLTILLIPHILKAQNDSDSYTRYELLD
                     VSTQSFRIIYDVSAVRSGDVYYFNTLRKGSE"
BASE COUNT         2448 a         1915 c         1765 g         2729 t
ORIGIN      
        1 cctgaaatca atgtaatgga attttccttt tccccgttta aaagtatcaa tgtgaataat
       61 tttcgagtct ttcccgtttt catccaccgg ccattgtttt ccattatcgc ccaattcatc
      121 ccatttcaca cctttaaaaa agggaactac ccggctgatt tcttccagcg ctgttcgtgg
      181 gtgataatcc ggctgctcat atcccatccg gttcattatc tccacaataa tctgtccgtc
      241 aggttttgtg ccagccagag gctctactac ctcatttacg cgttgtattc ttctttcacc
      301 attggtaaac gtgccattct tttccaggaa ggaagtcgcc ggtaaaatta cggtagcacg
      361 tttcgcggtc tctgtcatga acaactcctg caccaccagc aactccaaat tatccaacgc
      421 tttctttaca tgctgagtat tgggatcggt ttgaaccaca tcttcgccaa ttatccacaa
      481 cgctttaagg ttgctttcta tggcagcatc aaacatctgc gggattttta atcccacttt
      541 atctgaaaca ggtcttttat aaaaagtggt atacatggaa tgaatttcag gttgggtaac
      601 atccagatat cccgcaccct ggtgcggctg cacgcccata tctgccattc cctgcacgtt
      661 attttgtccg cgcagaggat ttacccctac gcctttctta ccgatatttc cggtaatcat
      721 ggcaaggttg gcaattaatt gaatcgcaaa agtaccctgg ctatgttccg taactccaag
      781 tccatggaat tccatggcaa gctccgcggc agcgtaagtg atggccgctg cccgcacttc
      841 ttctttatcc actccggtaa ttttagacaa ctcatccatg ttcagtctga gaatatcgga
      901 tttaaactca acaaagcctt cgcatcgctt tgctacaaat tcttcatcca ccaggttttc
      961 gttgatgatg taatacagca tcatgttgag tactgcaaca ttggttccgg gtcgaacctg
     1021 cagaaaatgt ttggcgtatt tcaccaattc aatttcaacc gggtcaatga taatcagcgg
     1081 aatacctttc atagctttct gcttgatttt tgccccggtg accggatgtg ctaccgtcgg
     1141 attcgcgccg atgactaaca tgcatgtggt atgatttata tcctcaatcg agtttgttgc
     1201 tgcaccggta ccaaatgatc gttgcattcc caatgccgta ggagagtggc aaacccgggc
     1261 gcagccatca atattattag tacctatcac agcccggata aatttttgca tcaggtaatt
     1321 ttcttcattg gttgcccggg cgcaggaaat tccgcctatg gaatcaggac caaattcgtc
     1381 tcggattgcg gtcagtttgc cggtaataaa atcatacgct tcatcccacg acacttcttc
     1441 aaatttacca ctccgcttta tcattggaga tctcagccgg tcaggatgat tatagaattt
     1501 gaaagcatat cttcctttga gacaagtatg accctggtta acttcggcat cccagggggc
     1561 gcgaatgctg acgatttcct gtcctttgga agaaacttcc aggttacagc ccacgccgca
     1621 ataagtacaa actgttctgg tggttttgtc cgcttccagt gcgaaggatt tgaatacatc
     1681 ggaaattgca ttggttgggc aattttggga acaagcgcca caggatacac attcggattc
     1741 agcaaaggta gtatccatac ccataattat tttggtgtcg aagcctcttc cggccgtgct
     1801 tagtacaaat tggccctgca cctcatcaca ggcgcgcaca caccggtaac aattaatgca
     1861 gccgctaaga tcggagatca tgtaggggtg gcttttatcc ggctttcgat cgaaatgatt
     1921 tctgcctttc ggataccgga tttgtgcagg ctcaatgccg agcatcccgg aaacagcctt
     1981 cagttcgttg tttatgggac tgtcggtctt aaatttttct tcaggataat cagtgaggat
     2041 aagttccaga atattcttac gcagggagct gattcgttcg gagttgggat agacataggt
     2101 attttcagaa atgggagtat gacaggaggc ctgaactttt ctattgccat tgtgcgttaa
     2161 tgcaacatct acgctacaaa cgcggcaggc tccgtagggt tttaaatttg gagcgtcaca
     2221 aagggtaggc accttacctt ttccgatgtg gcggttgaga aaggaaagaa tcgtttcccc
     2281 gggttttatt tcaaaaggaa tatcatcaat gaatgccagt ttaagatctg ttttaccatt
     2341 tatctgcttt ccctctttcg atttggttac tgtaatcgtc tccgaatttt tcatcacctt
     2401 aatttttaat gaaatatggt ttcaattcgt tttcaaaata ctgaagagca ttctttattc
     2461 ccagtggcag tccgccgccc aatgcgcaca atgatcctac ttcaagtgtt tccagcaagt
     2521 catccattaa ttttctgtcg atttggtaat cttcatgctg agcttttaca agcatttcca
     2581 ttccccgaac agaaccaatg ctgcagggaa cacatttacc gcatgactcg gccgcggtga
     2641 attcaaacaa atgctccagg tatttgatca ttggaaagtt ttccggtatg gaaactacgc
     2701 cggcgtgtcc aagcagaaat ccattttcgg caaaactctc aaaatccaga gtcagatcag
     2761 gaattttgtg aaccggaaca attcccccca aaggccctcc gatatgcaac gctttaatgg
     2821 gttgcttaaa accctgaccc aattcattta ccacttcctt caaggttgtt cccattgcaa
     2881 cttcgtacat tccgggacga ttgaaaaaac tgtccagaca aaccaatttg gaaccggaag
     2941 attttccggt gccgattcct gcatatactt taccgctatt attgatgata tacggaatac
     3001 atgccagggt ttccacatta tttactaccg tgggtttatt gaaaagacct tcctgggtag
     3061 ggtagggcgg cctcaccctt acatgaggcg gctgaccttc aatagaagaa agcaaagcgg
     3121 tttcttcacc acacacgtat gcgcctgcac cttcgattac tttaaatcta aaatcaaaac
     3181 cactgccttt aatattgtcg ccgagcaatc caagttcctc cagttccgca attgcaaggt
     3241 ttactgccga aacagattcc ggatattcac cccggatata aagtacgcct cttgatgccc
     3301 cggtaataaa tccattgacc atcattccaa ataaaacaag atgtggtctt tcttccatga
     3361 gatatctatc agaatatgca ccgggatcgc cttcatccgc attgcagaca ataaacttct
     3421 gatcagaagg agtattttta cacgattcta atttgaaact cattttaaaa ccagcaccgc
     3481 cacgaccccg aacttcagaa tcctttattt cttgtaacaa tttttcagga tcacgggaaa
     3541 atgctttaat aagccgagca taaaattctt gcagatcagg aaagggtgca gtgaggatag
     3601 gctccgccat attagttcct acgaagtatt gatctttatt ctttccggct gagccttgca
     3661 taatttcatc aatctcagag tccgatttgc cggaatagtt ttcaccccgg tagtaaaaag
     3721 atccattctc atgacacctg cccaggcagc acatgtgacc aatttcttct tcacccagat
     3781 gttccaggag ttttgttttt agattctcct gggttcctgc cagcatgcat acacttccgt
     3841 tgcagatgta agcctttatc tgtttgtttt ccggtttaag aaaatcgtag aatgtggacg
     3901 caccataggt tattgaatct ccaatcagaa aatctttggc aatttgacga agaacttcag
     3961 gttcgggcgt tccgtttttc tttccttctt ttaccagttc ttcaaacagg ttcctgtcca
     4021 gaccttttct cccagaaagt gcacttacat tatccgacat ttctaatacc cggttagttg
     4081 accttcaaga gtttagtttc gcttcaaaat ttactcaaat tagtcaaaat ctggcatttt
     4141 agaaaggaaa ttgagtttgg tgaccggccg gatatcagag ttaagtatag tattaatggt
     4201 gatgaccgcc tttgccatgc acatggccgt gatccaattc ttctttggtg gcttccctga
     4261 cttccatcac ctccacatcg aaattcagtt cacgacctgc aaggggatga ttggcatcta
     4321 cagtaacgac ctcggtttta acttccttca ccgttagtac ttgtccctga ttggtctgaa
     4381 attgcatccc cggctgaacc tcctggtcac caaatgattt tctggggaca tcctgtacaa
     4441 gtttgggatt aatttctcca taccctttct caggcctcac cttgatctta aatttatctc
     4501 ccctggccct gccctcaagt ccttcttcca tacccgggat taaatttccg acgccctgaa
     4561 taaaaagcag aggatccctg ccctgactcg aatcgatggt attcccatca ttgtcggtta
     4621 aagtgtaatg aattgcagct actttgtttt tggctatttg cataattact ctggtatttt
     4681 gtttaaaata ggagcggcaa agttagggct attattcagg aattgcagat tgaaaaaagg
     4741 ttaattgcag cgcttatcaa atcctattaa attagaaagt aaataaggat ttgtaattta
     4801 ttattaatca attatttata aaaaacaaaa accaattcac aaatgaaacg aatgttcact
     4861 tttctcctgg ccattatttg ctgtcaggct tatggaactg aagtaatcgg gaattacaaa
     4921 accgaggtaa ttgtcaatct tgatcgaagt gtattagtga aagagataat cgaggtaacc
     4981 gtggaaggga ttaacattcg ccggggtatt taccgggata taattaatgt gtaaattaat
     5041 gaacaaggtc ggagaatcag gtatcccgtt agtgtggtca gtgttatgaa agacggagtt
     5101 ccggaacctt actctgtgga tgaatttgat caatttgtac gtgttaaaat tggtaatccg
     5161 gatattttgc tggaaaaccg ggtgcataaa tatgagatta catacaccat gccagaacag
     5221 gtgcgattct ttgataacca tgatgaaatc tattggaatg ctatcgggac agggtgggac
     5281 tttgaaattc aatacgctga agcacgggtt ttcctgccgg atggagccat catcaccgaa
     5341 tatgcagggt acacagggta ttttcaagat accagttgtg aatgtgagat agtaattgtg
     5401 aacgaaaatg aattggaaat aaaaactacc ggatcattat atccgaacca gggcttaaca
     5461 gttgctgccg gctttagtaa aggagtaatt ccacctccta ctttatctga caaagacaga
     5521 tacctggtaa aaccagatct gatttatacc ctgggcgcga ttggattagg aatcgtatta
     5581 atctatttta tggtatcgtg gtacctggtt ggaaaagatc cggaaggagg aaccattgta
     5641 ccgctatttt actcaccgac cggtatttcc ccggcagcga gcagaagttt aacaaaaatg
     5701 gggtttgata ataaaagttt tactgcatct gtagtaaatc ttgcagtaaa aggaattatc
     5761 aaaattgaaa agataaaaga tgggtataag ctgcacaaac tggtggaaaa tccagctgat
     5821 ttacctatgg aggaaaaatc gatctttgat aaacttttga agcatcaaaa ttcgttcagc
     5881 ataggagata aatacagttc cactcttaat aaggcattgt caaatcataa agaatcctta
     5941 ataaaggaac tgtcgaaaac ctatttcaat acgaaccaaa aatggttgat acccggagtg
     6001 ctgatttcca ttgtaactat tttcgtttgt tttggtgcca ccttaaatta tgatgaaaat
     6061 acttttatag gattaatccc tataggaatg atttctataa tattcttccc tatcctggtg
     6121 gctattttca aaaagctaag tagtcttgga agcctatctc aagcactgac aatgctggga
     6181 ggacttgctt tcctgggttt catagggtat aactactttg gaatccttga tgctattgat
     6241 ttgcttggct cgatagatgt gtacgtagtt tatttttttg gaatactgct aatatctctg
     6301 cttacgctca attttctttt ctcttacctg ataaaagctc ccacagtttc cggaagaaaa
     6361 ttgatggaca aagtggatgg attaaagatg tatttaaatg tagcggaaaa agaccggctc
     6421 gaacaaatgc atgcaccgga ccagaccata gaacactttg aaaaattact cccttatgcc
     6481 attgcacttg accaggagaa acactggtct gatagatttg aaactttgct tgcatccgct
     6541 tcagaagggg gttatcaacc aacctggtat acaggcggta ctatccgggc tattactttt
     6601 agtacattaa ccagggatat tggaaggggg tttaattctt ccgtttcatc ggcgagcaca
     6661 cctcctcaaa gcagtagttc ttcaggcagt tccggaggcg gatcttccgg cggtggcggt
     6721 ggcggtggtg gtggcggcgg ctggtaatgg attatgtttt atatttactg aatgaaatta
     6781 cctaaactac ttgttggcat tgtattgctg gttattggca tttttgtaat ctactgggcg
     6841 tacaaccaca gtccggaagc aggcctgggt gacagaattg tagatgccct ggagggagaa
     6901 aaggttttaa gtaaaactac ctattacacc atgttggttt ccggtacttt actgattatc
     6961 ctgggtataa cccgaactgt aaaatcactt taagaggaaa tttatttcat tctcctcagc
     7021 tcagcttcag ccgcaataaa gccaaaggca gaccaacttg taccactaac cacctgggta
     7081 aatatctctt tggctttttc tctgtcgcca ttaatcagat accagttacc aactccatat
     7141 ccctgagttg caattgtaac tccattatcc tcctgatccc cggcaacttg taataattct
     7201 tccggagttt tagttccttt atacataagc aacctgttta ggtaggattc attttcaata
     7261 atttccatgt cggctgaaat tgcatccaaa agttttttgg catcaatatc ccgccttatt
     7321 ttccggtagc tcatataaag ccaatctgta gtagcaacca gtaagtcgtt attgtcagaa
     7381 actttcatgc attccaggta agtattgata gcttttcgat aatcacgttt tagataatga
     7441 gctaaagcca ggtgatagta cacattaaac tgcgtagttg ataaagggat attcaattta
     7501 ttaggcgctc cgtcaggttc aatcattaat tcttctcctg caataaacca cgcggctttt
     7561 tcaaggtcat ctatggcttt gtcaaactgg cgggtagata tgtaacgatg cccgcggtgg
     7621 cggtataact taaatgattt cggatgcaat tccagaccct tgctaaatac ttcaatagct
     7681 tcaggataca gggacagata agccagccgt cgaccatacc agatgatatt catttcatcc
     7741 gttgaatcag cttcataatt ttgtctggca gaatccaggt tggcagttaa tcttgccaac
     7801 gtttccggtg caagttcagg cggatacaaa gcatttccat ctaatgaata agcggcaggc
     7861 tttttctcac taccatcgac ttcttcatca attttatttt gatcatttcc aacactgcac
     7921 gcgttaatcg caattaagaa aaagaaaaat tgaaaatact tcattgagtg aaattcaatt
     7981 tttaatgttg aatgtaactt ttgacctgtc ccaaagcata tttaattgca aaaggctgtc
     8041 tgattccgta aaattaatgg tgaactgttc aatctcggta tctgtttctg aaactctgcc
     8101 cttgaccctt acaacatcca gctcttcttc aaacggattt ccaactaatg ttctccccca
     8161 ataattggtc ttggaattga ttgctaaaac ccattcagtt tttccgggaa ttgtgtagag
     8221 tgaatatatt ccttttttca ataatgaatc ctccaggtaa atattttgat taaaagaaat
     8281 ttgtgtggcc tcattggctc ctgtgcgcca tttttctccg aaaggaacta gcgctttttc
     8341 accttcccca aaaatcaacc ttcctttttt gtaaggcctg ctataagtta ttgcaatact
     8401 cagggattca ttggtgtatt caactacctc tgcaggacta tgactttttg tggtggcaag
     8461 caggtatata ttgtatatga taacaagtcc tgcgataatg gcgagtataa tgaatatctt
     8521 ctttagcata gaattggtgt ttcgattatc taagataacg gaataatgct tcagctgatt
     8581 agtgattccg acaataattc ataaattgat aaccctaaat ttcgtacccc atgaaatatt
     8641 tatatcaaaa acaatttcaa aatgctttaa gtgcatgtgc attacttacg attttactta
     8701 tacctcatat tttgaaagcg caaaatgatt cagacagcta tacgcggtat gaattattag
     8761 atgtttctac tcagagtttc agaattatct atgatgtaag tgcggttcgt tccggggatg
     8821 tatattattt caatactttg agaaaaggca gtgaaca
//
LOCUS       DASWHX010000225         3742 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_3240274, whole genome shotgun sequence.
ACCESSION   DASWHX010000225 DASWHX010000000
VERSION     DASWHX010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 3742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3742
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3240274"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..402)
                     /gene="era"
                     /locus_tag="VGA21_14555"
     CDS             complement(<1..402)
                     /gene="era"
                     /locus_tag="VGA21_14555"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007656142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEX9651781.1"
                     /translation="MTTISHKAGFVSIIGKPNVGKSTLMNQLVGEKLSIITSKAQTTR
                     HRILGILSGENFQIVYSDTPGIIKPAYKLQESMMTFVKTAVSDADIIIYIVELGQKFD
                     PDDEVIRRIKKSEKKLILVINKVDLDKDKLLE"
     gene            complement(412..2457)
                     /locus_tag="VGA21_14560"
     CDS             complement(412..2457)
                     /locus_tag="VGA21_14560"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008627958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEX9651782.1"
                     /translation="MKSFVLSLIVFILPCNSFSQDLKNPSFEEVLSLQSVGGHTLSPD
                     GKSIVYSINSTDWENNQYDTELWLSRNGEHPFQLTNNTKGSSSGVRWSPDGKWIAFVS
                     RRDDGNQIYIINPIGGEAIKITKEKDGVNDFEFSPNGKSIAFTKTTEAKSNKQREDKF
                     GKFSVEDNEYNLPTLWLMDFNPEDFFKAQLPNYTKDSVSKELEKPKALIDNPKFAIRR
                     YEWSPDGKSIALNHAPNTDILGFMKTDISIFDVETKKVNPLLQNSAGDFFAAWSPDNK
                     AILYTSGVNDTTSNFFKNAKVFIINTDGSDNRQIATGLDEDLGGFVWTPLGIFATASI
                     RTNRHLYKIDPKSGNIDIVNVNHNRIYGLSFSKDGSIMSFNGQRDNELTELYTCPTMN
                     FKITQLTSLTKQINGWKVAKSEVQTWQSEDGTAVEGVLHKPADFDPGKKYPLLVMIHG
                     GPTGVDRPTPVLTYVYPLIQFLNKGAVVLRVNYRGSAGYGEAFRSLNVRNLGVGDAWD
                     VLSGIDNLISKGFIDESKMGCMGWSQGGYISAYLTTNTNRFKAISVGAGISNWMTYYV
                     NTDIHPFTRQYLEGTPWNDPEIYAKTSPMTTITKASTPTLIQHGEFDRRVPIPNAYEL
                     YQGLQDVGVETKLIVYKGFGHGITKPKERLAAVWHNWQWFLKYVWGEEVEIPIEEEK"
     gene            complement(2638..>3742)
                     /locus_tag="VGA21_14565"
     CDS             complement(2638..>3742)
                     /locus_tag="VGA21_14565"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX9651783.1"
                     /translation="AGSVILVMRKGKVAYFEAFGYRNVEDKLKMERNTIFRIASQTKA
                     ITSAGIMMLQEQGKLLISDPLSKYIPEFEKTKVAVQKENESEYEIVDANRQITIRDLL
                     THTAGIGYGWGLAEKEWEDAGILGWYFADRDEPISTTVGRMASLPMSEQPGQKFVYGY
                     GTDILGVVIERASGMPLDAFIELNILKPLGMEDTYFYLPPQKANRLSVVYSATNAGGI
                     TKAPDPGKMIGQGAYLEGPRKSFSGGAGLLSTAEDFARFLLMMFNEGELNGHRLLAPS
                     TIKLMTSNHLRDIPYSPGRGFGLGFEVVLDAGSRGIPGSVGEYGWGGAYGSNYWVDPE
                     EKLIIVYFKQLIPTNGLDDAGKLRSLIYQAIVD"
BASE COUNT         1036 a          832 c          703 g         1171 t
ORIGIN      
        1 ttcaagaagc ttgtctttgt caaggtcaac tttattgatc accagtatca attttttttc
       61 ggatttttta atccgccgga tgacttcatc atcaggatca aatttttgtc ccaactccac
      121 aatgtaaata ataatgtccg cgtcgcttac agcagtcttt acaaaagtca tcatggattc
      181 ctgcaattta tacgcgggtt ttataatgcc tggtgtgtct gagtaaacaa tctgaaaatt
      241 ttctccactt agtatgccaa ggattctgtg gcgggtagtt tgagctttgc ttgtgataat
      301 agacaacttt tcacccacaa gttgattcat gagggtagac ttgcccacat ttggtttgcc
      361 aataatggaa acaaatcctg ctttatgtga aattgtggtc aaactatttt tttacttttc
      421 ttcttctatc ggaatttcca cctcttcacc ccagacatat tttagaaacc attgccagtt
      481 gtgccaaacc gcggcgagcc tttcttttgg ctttgtgatt ccgtgaccga agcctttgta
      541 aacaattaat ttggtttcca cgccgacatc ctgcaaaccc tggtataact cataggcatt
      601 gggtatgggt acccgtcgat caaattcacc atgctgaatt aatgttggag ttgaagcttt
      661 agtgatagta gtcatgggag atgtttttgc atagatttcc ggatcgttcc agggtgtacc
      721 ttccaggtat tgccgggtaa atgggtgaat gtctgtattt acataataag tcatccagtt
      781 ggaaataccg gcacccaccg agattgcttt aaatcgattg gtgttggttg tcagataagc
      841 ggaaatataa ccaccctggc tccagcccat gcaacccatt tttgattcat caataaaacc
      901 cttgcttatt aaattatcaa tccccgaaag aacatcccaa gcatcgccca cccccaggtt
      961 gcgaacgttc aatgaacgaa aagcttcccc ataaccggct gagccccggt aattaacccg
     1021 taatacaacc gcacccttat ttaaaaactg aataagagga tatacataag taaggactgg
     1081 tgttggccgg tcaacgcctg ttggtccccc gtggatcatc accaacaagg gatatttttt
     1141 accgggatca aaatcagcgg gtttgtgcag cactccttcg accgcagtac catcctcact
     1201 ttgccaggtt tgtacttcac ttttagcaac cttccatcca tttatctgtt tggttaaaga
     1261 tgttaactga gttattttga aattcattgt ggggcaagta taaagttcag tgagctcatt
     1321 atccctttgc ccgttgaaag acattataga gccatcttta ctaaatgaaa ggccataaat
     1381 tcgattatgg ttcacattaa caatatctat atttcctgat ttgggatcga ttttataaag
     1441 atgccggttt gtgcgtattg aagcagtagc aaaaataccc aaaggagtcc atacaaatcc
     1501 gccgagatct tcatctaaac ctgttgcaat ttgtcgattg tctgatccgt ctgtgtttat
     1561 tatgaaaact tttgcattct taaaaaaatt actggtggtg tcatttacac ccgatgtata
     1621 caaaatagct ttgttgtcag gagaccaggc agcaaaaaaa tctccggcgg agttctgtaa
     1681 taatggattg acctttttag tttctacatc gaagatagag atatccgttt tcatgaatcc
     1741 gaggatatct gtgttaggtg catgatttaa ggcaatagat ttgccatcag gagaccattc
     1801 atatctccgt attgcaaatt tgggattatc gattaacgct tttggctttt ccagttcttt
     1861 tgaaactgaa tccttagtat aattgggcag ttgagctttg aaaaaatctt cggggttaaa
     1921 atccataagc cataaggtcg gcaagttgta ttcattgtct tccacagaga acttcccaaa
     1981 tttatcttcc ctttgtttat tggatttagc ttcagtggtt ttggtaaaag ctattgattt
     2041 tccgtttggt gaaaactcaa aatcgtttac gccatccttc tctttggtaa ttttaatggc
     2101 ttccccacct attggattga taatataaat ctgatttcca tcatctctgc gggacacgaa
     2161 tgcaatccac tttccgtccg gagaccaacg cacgcctgaa ctgcttcctt tagtattatt
     2221 agtgagctga aatggatgct caccattcct ggaaagccaa agttccgtat cgtattgatt
     2281 attttcccag tccgtagagt taatggaata aacaatactc tttccatcag gagataaggt
     2341 gtgtccacca accgactgga gtgataatac ttcttcaaaa gaagggtttt tgagatcttg
     2401 ggaaaaactg ttacatggaa gaatgaaaac tatcagcgac aacacaaatg atttcataca
     2461 cgataaaatt taatttgtaa aagatacata attattggtg ttgttcacag ttgttctttc
     2521 ctggaatcga attccgattt ctgttttatt gcctgtaggg cctgggtttg aatcaaatca
     2581 agaataaggc aacgactttg aaggtttcag gttccgagca ataaacgcta aaaatactca
     2641 gtccacaatg gcctggtaga ttaaggacct caatttacct gcatcatcca aaccattggt
     2701 tggaataagc tgtttgaaat aaacaataat cagtttctct tccgggtcaa cccaataatt
     2761 tgaaccgtac gcaccacccc acccatattc tccgactgaa cccggtatcc ctcgtgatcc
     2821 ggcatcaagc acaacttcaa atccgagccc aaatcctctg cctgggctgt agggaatatc
     2881 ccggaggtga ttggacgtca ttaattttat agttgatggc gccagtaacc tatgtccatt
     2941 cagttctcct tcgttaaaca tcatcagcag aaatcttgca aaatcctcag ccgtagagag
     3001 gagtccagca cctcccgaaa agctttttct agggccttcc aggtatgcac cttgccctat
     3061 cattttacca ggatccggtg ctttggtaat tccaccggcg ttagtagcag aataaacaac
     3121 cgataatcga tttgctttct gcgggggcaa gtagaaatac gtgtcctcca tacccagcgg
     3181 tttcaaaata ttcagttcga tgaatgcgtc aagaggcata ccgcttgctc tttcaataac
     3241 aactcccaaa atatctgtgc catacccata cacaaatttt tgccctggct gctcactcat
     3301 aggtaatgaa gccatccttc caacggtggt ggaaatgggc tcatccctgt cagcaaaata
     3361 ccaacccaaa attcctgcat cctcccattc tttttcagca agtccccaac cataaccaat
     3421 tccagctgtg tgagtaagca aatcccgaat ggtaatttgt ctgtttgcat ctacaatttc
     3481 atattcactt tcattttcct tttgaacagc aactttagtt ttctcaaact ccggaatata
     3541 tttgctgaga gggtcggata tcaatagctt tccttgctcc tgcaacatca taattcccgc
     3601 gcttgtaatg gcttttgttt gcgatgcaat acggaaaatg gtatttcttt ccatttttaa
     3661 tttgtcctcc acgtttctat aaccaaaagc ttcaaagtaa gctacttttc ccttacgcat
     3721 cacaaggatt acgcttccag ct
//
LOCUS       DASWHX010000226        12439 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_3581437, whole genome shotgun sequence.
ACCESSION   DASWHX010000226 DASWHX010000000
VERSION     DASWHX010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 12439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12439
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3581437"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(38..181)
                     /locus_tag="VGA21_14570"
     CDS             complement(38..181)
                     /locus_tag="VGA21_14570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651784.1"
                     /translation="MDALNDLAAGMGILTAGVAAAVVLTYVVNTIGRRFNNGENLIDM
                     DLF"
     gene            441..920
                     /locus_tag="VGA21_14575"
     CDS             441..920
                     /locus_tag="VGA21_14575"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX9651785.1"
                     /translation="MIRFFESTDLDQITGIFNEVVETGEYFFTEFPLTAEQMRERLST
                     PERETVVLEDDGAILGFYYMNPLFEGRSNHVANCAYAVSKSARGKKVGSRLAEFSIEH
                     ARKKGYKSISFVAVVATNEVANHLWQKLGFRMVGTMKKSHRNKDGSFVDTNIYQLEL"
     gene            complement(924..2060)
                     /locus_tag="VGA21_14580"
     CDS             complement(924..2060)
                     /locus_tag="VGA21_14580"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007659321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HEX9651786.1"
                     /translation="MIRIFQCLIILAVAIILGCSGSNQETEMEKEPPEHLAESKPYGT
                     FGGKEVTQFILRNSAGMEVGILNYGGTVTHILTPDKNGNAGDVILGFENLEGYLQDDN
                     PYFGCLVGRYANRIANATFELRGETFQLAANNDANSLHGGLRGFDKVVWDAELMPGDS
                     SLRLKYLSHDGEEGYPGNLQVEVTYALSSDNELVIKYTATTDQPTPVNLTNHCYFNLS
                     AGTDETILDHELMLDADKFTEVNENLIPTGNLADVDSTPMDFRNLNRIGNDIMKVAGG
                     FDHNWVLNKSGEEIEKAGELHHSASGRVMEIFTTEPGIQFYSGNFLDGTLRGKAGKIY
                     FKHGGLCLEAQHFPDSPNQPEFPNTILEPGNTYRQTTIYKFSIR"
     gene            complement(2057..3988)
                     /locus_tag="VGA21_14585"
     CDS             complement(2057..3988)
                     /locus_tag="VGA21_14585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018622331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-L-arabinofuranosidase domain-containing
                     protein"
                     /protein_id="HEX9651787.1"
                     /translation="MLPIASYAQEVSRKFNPVNFSKVEVIDNFWKPTMDLVATETLAA
                     CIEQTEVKTPRIRNFEKVARDKSEAHEGIFYDDSDVFKALEAIAYSINNYPDPALEKK
                     ADEWIDKIAAAQLEDGYLNTYYTLGRLDQRWTDMSMHEDYNGGHMIEAAVAYFNATGK
                     RKFLDVAIKWAVHFDSLFGPGKRHWVTGHQELELALVKLYHTTNDKKYLDLAYWLLEE
                     RGKKLAKGYTWSSWRDTAYAQDVIPVRDQTEITGHAVRAMYMYTGAADVASLTGDEGY
                     MKAMLTVWGDVTNRNMYITGGIGASGRNEGFGIDYDLPNEQAYCETCASVGMVFWNQR
                     MNLLTGDSKFIDILERSLYNGARDGLSLSGDHFFYGNPLASRGQHARREWFGTACCPA
                     NIARLIASIGDYIYGYSDKGVWVNLFVGSKTNINVNNSEIGIEMQTNYPWDGNIKITV
                     NPKSRAEFPLYVRIPGWVQGKPVPGDIYKYGDNIDTPYKVSLNGKEIPVAIEKGYAVI
                     NNKWKKGDEVQLVLPMEVRRVVAREEVEQNNDRVALQRGPLVYCVEGVDNDGQAWNLI
                     VPENTRFTSEFNPKMLGGITTIQFEIPVINVGSDGMSIQTQNKTITAIPYYTWANRGR
                     SEMQVWLPVRINDVKINYR"
     gene            4301..6169
                     /locus_tag="VGA21_14590"
     CDS             4301..6169
                     /locus_tag="VGA21_14590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015265696.1"
                     /note="Members of the Solute:Sodium Symporter (SSS), TC
                     2.A.21 as described in tcdb.org, catalyze solute:Na+
                     symport. Known solutes for members of the family include
                     sugars, amino acids, nucleosides, inositols, vitamins,
                     urea or anions, depending on the system; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/solute symporter"
                     /protein_id="HEX9651788.1"
                     /translation="MNSGFSNIDYIIFFGYCALIITIGLYVSRNKGKEKTAQDYFLAS
                     RSLTWWAIGASLIAANISAEHFIAMSGSGFAIGLGIAAYEWIAAISLIIVAKFFLPVF
                     LKNEIYTIPQLAEKRFNKHVGLFFASFWLLVYVFVNLTSVSYLGALALEKIVGIPLIY
                     GIIGLLLFSGIYSIYGGLTSVAWTDVIQVIFLVFGGLVTTFLALDAVGGGSIIGGFSY
                     IFEAGRDHFSMIISEGQIMVPDGGGGTKDAFNDLPGLAVILGSMWITNLGYWGYNQYI
                     IQKGLAAKSIEEAKRGLIFAGYLKILVPLLVIIPGITAYVLVNDFTPEQLAAMVGKPV
                     EELGVIAKSDEAYPWLLQNFVPAGIRGLAFAALTAAIVSSLASMINSTSTIFTMDIYK
                     HYINKAASNKQMVRVGRLVAIVAMLIAISFAPQLSNLDQVFQYIQEYTGFIYPGVVVV
                     FTMGLFWNQITSKAAIWTAAATIPIGIAIKFALPEIPWILRMGYVFIILCTVASVISF
                     LDKGVKVKSPTLGEGVQKKLISTSYLLTALSIVCILPGLFFSGTYAHLGFESIFVLAS
                     IFLMLAIISYTNAKIDVEDRKAFHTEVTIFKTGVGFNVGAIGIIAIVAFLYIVFWM"
     gene            6180..7679
                     /gene="araA"
                     /locus_tag="VGA21_14595"
     CDS             6180..7679
                     /gene="araA"
                     /locus_tag="VGA21_14595"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012925839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HEX9651789.1"
                     /translation="MLPNLKKLEVWFLTGSQHLYGDDVLKQVAAHSREIISSINLSGQ
                     IPVKIIARQTLTTPELIYQVCLEANSSSGCIGVILWMHTFSPAKMWIRGLKILQKPLL
                     HFHTQFNAEIPWEVIDMDFMNLNQSAHGDREFAHICARLNLKRKIIAGHWKDKSCLKQ
                     IGIWTRAAIARYDLDGARIGRLGDNMRYVAVTEGDKVEAEIKLGFSTNGYGISELAEL
                     ITKITNARAEKLVYEYLDEYQVVRELKKGGPKYSSLVYAARIELGLRTFLKKENLVGF
                     TDTFEDLHGLEQLPGIAVQRLMRDGYGFGAEGDWKTAALVRAMKVMSCGLEGGNSFME
                     DYTYNFTSVENQVLGAHMLEICESLAESKPSCEIHPLSIGGKSDPVRLVFNVGAGKAV
                     NASLIDTGSRFRMLVNKVELRKPEKALPKLPVARAVWKPLPDLKTSATAWLMAGGSHH
                     TCLSKALEVEHLMDFCEMMNIELLVIDESTNLVNFHNELRWNDKSFEEK"
     gene            complement(7686..10109)
                     /locus_tag="VGA21_14600"
     CDS             complement(7686..10109)
                     /locus_tag="VGA21_14600"
                     /inference="COORDINATES: protein motif:HMM:NF026255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX9651790.1"
                     /translation="MRIGIAIVLKLILVLMLANTAFGQEKSAVITGTLIDINTKETLP
                     YTTVAVYRTDSTLVDGAITDFDGAFRLEVEPAQYYLEIQSISYELSRILVTVADGESR
                     SLGTITMRQAVKLLEEVVVTGEKNQMRLELDKRIFNVGQNINNVSLNAADILDNLPSI
                     NVDVEGNVSLRGNQNVRILVDGKPSGLVGISGPDALRMLQGDLIESVEVITNPSARYD
                     AEGTAGIINIILKKQERMGFNGSFNINGGLPVDVGISTNFNYKVGKFNFFANYGIDYD
                     KRPGGGSSYQKFTFPDTTYVTYRDQDRERGDFGNSFRFGIDTYITDNDVITASGLYRI
                     SDESNDSRIIYDDYSGDGVLLDKTLRLDNETEDDQNSEFQLVYRKTLPGDGHELAAEV
                     QFRDNSETEKSQISESDALTNNAPYLFQRSSNREGDQNWLAQLDYVYPVNEAFKFEAG
                     YRGTFRQMESDYLVEEQNTSGNWISLDNFSNQFIYDEDVNAGYAIFEHKLPKWGYQVG
                     LRAENTRIKTYQRETAEESKKSYLNFFPSVYLTYKLSELNTIQVSYSRRLSRPRHWFL
                     NPFLSFNDPRNIRTGNPNLDPEYTNSYEAGYLNNREKTSLYGGIYYRYTSGMIQRINY
                     VETNGTTYSQPHNLGSEESYGIELNVSSDPLEWLNLNGNANFYHAKVDGDFNGERLSS
                     ETYTTQFNLSTKFDLGKVDLQLRGRYRAPENTTQGRRKSQYHADIGANWLILKGNGTI
                     SLNIRDLFNTRKYRGISEGQYFYSESEFQWRSRQLRITFTYRINQKRKDNGEDNDDNG
                     GNDDMEFQN"
     gene            complement(10524..11777)
                     /locus_tag="VGA21_14605"
     CDS             complement(10524..11777)
                     /locus_tag="VGA21_14605"
                     /inference="COORDINATES: protein motif:HMM:NF025854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX9651791.1"
                     /translation="MRLIYKIVILYLLITLGVIIAGGIITYVNIQTQVNLEQKRHLRE
                     QLGYLKNSIEKGVPIESLNHEKVSITRVPQGVGEERIEFTDTVVIHATLRRLEPHVKL
                     KTITEVSGTFYRFEMYDVIVEAEDIIEGVYKSMLGIYAILFVAVIIITFIFSARVFKP
                     FNKTLEFISGFSLSKSDPLDLRKTSTREFTQLNEFISEMTRKVKMDYQSLKEFTENAS
                     HEMQTPLAIMNSKLQLLIDSPNLNDNELKQILSAQEALTKLSRLGHSLALLTKIENQE
                     FNKLEEVNMSALVEKTLDDFTELIEIKGLTLSKNIDGNVKVHSDQVLMDVIIANLLQN
                     AIKHNIEKGKIRVSLNAESLTIENTGNVPKKDPALFFERFRKEDQSGDSLGLGLAIVQ
                     KICEANNFQISYNYTDNKHVLTVKF"
     gene            complement(11774..>12439)
                     /locus_tag="VGA21_14610"
     CDS             complement(11774..>12439)
                     /locus_tag="VGA21_14610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020526681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX9651792.1"
                     /translation="EDNKSLSESIEAFMTREGVVCENAYNYKQAIDKIISFDYDVVIL
                     DLMLPDGDGLDILKTIKEERKQCGVLILSAKNSTGDKIKGLNLGADDYLAKPFQLTEL
                     NARLNAINRRKKFDGHSEIQFKEMRIDTVKEEIKVHGKPVTLTRKEYELLLFFLSNKE
                     RIITRQTIAEHLWGDYMDLNDSFDFVYTHIKNLRKKLTAHGCRDYITTRYGAGYKFKS
                     TIE"
BASE COUNT         3415 a         2640 c         2447 g         3937 t
ORIGIN      
        1 cgtttggttg agcgttgaat taagaatatc tgttcgatca aaataaatcc atatcaatga
       61 gattctcccc attgttaaat cttctgccaa ttgtgttgac aacataagtg aggacaactg
      121 ctgcagcaac tccggccgtg agtatcccca ttccagccgc gaggtcgttc aatgcatcca
      181 tgtgttagtt cgtttaagtt gagaatgatt agattatttg attggttccc aaagatcaga
      241 ctagttatta attatttgta aatcagatta ttagagtttt cagaattcaa aaattttgtc
      301 atattgaaaa ttcctttttc aaatcgaatc tcaggtaatt aacattatta aataatattg
      361 tagcttcaaa tgttgatttg ccaacaacat ctttgaccaa ttgaattgtt gaacacaaag
      421 ctgactttaa ttctgactta atgataagat ttttcgagtc aacagatctt gatcaaatta
      481 ccggcatttt taatgaagtt gtggaaacag gagaatattt cttcaccgaa tttcctctta
      541 ctgcggaaca aatgcgggag cgattatcaa caccggaaag agaaaccgtg gtcctggaag
      601 acgatggagc gattcttgga ttttattata tgaatccttt atttgagggc agaagcaatc
      661 acgttgccaa ctgtgcctac gcggtaagta aaagtgcccg cggtaaaaaa gtaggtagca
      721 gacttgctga gttttctatt gagcatgccc gcaagaaagg gtataagtcc atttcttttg
      781 ttgccgtggt tgctactaat gaagtagcta atcatctctg gcaaaagcta ggtttcagaa
      841 tggtgggcac catgaaaaaa tcgcatagaa acaaagacgg ctcttttgtt gacacaaata
      901 tttaccagtt ggagttgtga aggttatctg atcgaaaatt tataaatggt ggtttgcctg
      961 taagtatttc ccggttccaa aatcgtattg ggaaattcag gttgatttgg tgaatcagga
     1021 aaatgctgcg cttcaagaca caaacctcca tgcttaaaat agatttttcc cgccttaccc
     1081 cggagggttc catccagaaa atttccggaa tagaattgaa ttcccggctc agtggtaaat
     1141 atctccatta ctcttccact tgccgagtgg tgcaattcac cagccttttc tatttcttct
     1201 ccggatttat tcaaaaccca attatgatca aatcctcctg ccactttcat gatatcatta
     1261 ccaattcgat ttagatttct gaaatccatt ggtgtcgagt ccacatccgc caaattacct
     1321 gtgggaatca ggttttcatt cacttctgta aacttgtctg catccagcat aagttcatgg
     1381 tccagaattg tttcatcggt tccagcagat aaattaaaat agcagtggtt ggtcaggttt
     1441 accggagtag gctggtcggt agtggctgtg tatttaatta caagttcatt gtcagaactt
     1501 aatgcatagg tcacctccac ctgcaaattt cccgggtatc cttcctcccc gtcatgacta
     1561 agatatttca aacgcaggct gctgtcgccg ggcataagtt ccgcatccca aaccaccttg
     1621 tcaaaacctc ttagaccgcc atgcaaacta tttgcatcat tatttgccgc caattggaag
     1681 gtttctcccc tcaattcaaa tgttgcattg gcgatccggt ttgcatatct gcccaccagg
     1741 cagccgaaat aggggttgtc atcctgaaga taaccttcta aattttcaaa ccccaggatt
     1801 acatcacctg catttccgtt cttatccgga gttaagatat gggtaacagt acccccatag
     1861 ttcaatattc ctacttccat tcctgctgaa ttcctgagaa tgaactgagt aacttccttc
     1921 ccaccaaagg ttccataggg ttttgattca gcgaggtgtt ccggaggttc tttttccatt
     1981 tcagtttcct ggtttgaacc tgaacatccc agaataatgg caactgcaag aattatcagg
     2041 cattgaaaaa ttcttatcat ctgtaattta tcttaacgtc attaattctt accggaagcc
     2101 atacctgcat ttcactgcgg ccccggttag cccaggtgta ataagggatg gccgttatag
     2161 ttttattttg agtttgaatt gacattccat cgctgcccac attaattacg ggaatctcaa
     2221 attgtatggt tgtaattcca cccagcattt tcggattaaa ttctgaagtg aatcgcgtat
     2281 tttccggaac tatcaggttc caggcctggc catcattatc cacaccttca acacaataaa
     2341 ccagcggacc ccgttgcaag gcaaccctgt cattgttttg ttcaacttcc tccctggcca
     2401 ccactcttct tacttccatt ggaagaacta actgcacttc atcccctttt ttccatttgt
     2461 tatttatcac cgcgtatcct ttttcaatgg caacaggaat ttcttttcca ttcaagctta
     2521 ctttgtatgg agtatcaata ttatcgccat atttgtaaat atcaccagga acaggttttc
     2581 cttgtaccca accgggtatt cttacgtata acggaaattc agctcttgat ttgggattta
     2641 cagtaatttt aatattgcca tcccatgggt aattggtttg catctcaatt ccaatttccg
     2701 aattattaac attaatgtta gttttacttc ctacaaaaag atttacccaa actcctttat
     2761 ctgaataccc ataaatataa tctccaattg aagcgattaa cctggcaatg ttggccggac
     2821 agcaggcagt accaaaccat tcgcgccttg catgttgacc ccgcgaagca agcggattcc
     2881 cgtaaaagaa atgatcaccg ctcaaagaaa ggccatcgcg tgctccattg tataaacttc
     2941 tttccagtat atctataaat ttactgtcgc cggtaagcaa gttcattcgt tggttccaga
     3001 aaaccattcc cactgatgcg caagtttcac aatatgcctg ctcgtttggc aaatcataat
     3061 caattccgaa tccctcattt ctaccggacg caccgattcc cccggtgata tacatgtttc
     3121 ggtttgtgac atcgccccag accgttagca tagctttcat atacccttca tccccggtta
     3181 gcgaagcaac atccgcggcg ccggtataca tgtacatagc tcttactgca tgacctgtta
     3241 tttccgtttg atccctaacc ggtattacat cctgggcata cgcggtgtca cgccagctgc
     3301 tccaggtata tcctttcgcc aatttttttc ctctttcttc caaaagccaa taagcaagat
     3361 ccagatactt tttatcattg gttgtgtggt aaagcttaac aagcgccagc tccaattcct
     3421 ggtgaccggt aacccaatgc ctcttcccgg gaccaaacaa actgtcaaaa tgaaccgccc
     3481 atttaatggc cacatccaga aatttacgtt tgcctgttgc attgaagtaa gccaccgcgg
     3541 cttcaatcat gtgtccgccg ttataatctt cgtgcatgct catgtcggtc catctttgat
     3601 ccaatcgacc cagggtataa taagtattca gataaccatc ctccagttga gccgcggcaa
     3661 tcttgtcaat ccattcatct gcttttttct ccagggcagg atcaggataa ttattgattg
     3721 aataagcaat agcttccagc gccttgaaga catccgaatc atcataaaaa attccttcat
     3781 gagcctcact tttatcgcgg gcaacttttt caaaatttct tatccgggga gttttcactt
     3841 ccgtttgctc aatgcaggct gccagcgttt ctgtggcaac taaatccatt gtgggtttcc
     3901 aaaaattatc tatgacttca actttagaaa aattaacagg attaaatttt cttgaaactt
     3961 cctgcgcata ggaagcaatt ggtagcatta ttaaacaaaa gcttacgatt atactctttg
     4021 gtttcatatt ctgaatatta aacattggac gattacgttt ttaaagttaa aaataataat
     4081 ggttatataa agagcaaggt gaccaactaa tcctgaaaca ggttgacttc atcatttcac
     4141 gggggtcaaa tcattaagga tgcaggtgaa gttctaaaat ttaattaaaa taatgctatc
     4201 ttaatgcctt tcaaatgcgg acctgaaagc agcatcacac aatcggattt ggaaatataa
     4261 agaaggaaac tattacgttc ggaataataa ttaatgcaat atgaatagcg gattttcaaa
     4321 tattgactac atcattttct tcgggtattg tgccctgatc atcaccatag gattatatgt
     4381 ttcacgaaat aaaggaaaag aaaaaacagc ccaggattac tttcttgcca gccgatcact
     4441 aacctggtgg gcaatcgggg cttctcttat cgcggctaat atttccgcgg aacattttat
     4501 cgccatgtcg ggttccggat ttgccatcgg gcttggaata gctgcctacg aatggattgc
     4561 cgccatctcg ctaatcattg ttgcaaaatt cttcctgcct gtattcctta agaatgaaat
     4621 ttatacgatt ccccagttag cagagaaaag gtttaataag catgtgggac ttttctttgc
     4681 ttcattttgg cttctggttt atgtttttgt gaatttaact tccgtaagtt atctgggggc
     4741 actggctttg gaaaaaatcg taggaatacc cctaatctat ggaattattg gtttgctcct
     4801 tttctcggga atctattcaa tttacggggg attaacatcc gttgcctgga cagacgtaat
     4861 ccaggtgata ttcctggtct ttggcggcct ggtaaccaca tttctggcgc tggatgctgt
     4921 aggaggcggc agtataatcg gcgggttcag ctatattttt gaggccggca gggatcattt
     4981 ttccatgatc atcagcgaag gtcaaataat ggtgccggat ggaggtggcg gaaccaaaga
     5041 tgcttttaac gacttgccgg gactggcggt gatcctgggt tcaatgtgga ttactaacct
     5101 tggctactgg gggtacaacc agtatatcat tcaaaaaggc cttgccgcca aaagtattga
     5161 agaggctaaa cgcggattga tcttcgccgg atatctgaaa atcctggttc cactgcttgt
     5221 aattataccc ggaattaccg catatgtttt ggttaatgat tttactcctg aacagcttgc
     5281 cgctatggtc ggaaagcccg tggaagaatt aggagtaata gccaaatccg acgaggctta
     5341 tccctggttg ctgcagaatt ttgttcccgc cggaattcgc ggccttgcct ttgctgctct
     5401 aacggccgcc atagtttctt cgctggcatc aatgattaac agtacttcta cgatctttac
     5461 catggacatc tataaacatt atattaataa agcggcatcc aacaagcaaa tggtgagagt
     5521 tggcagactt gtggcaattg ttgccatgct gattgccata tcgttcgcgc cccagttaag
     5581 caacctggac caggtttttc aatatattca ggaatatacc gggtttattt accccggtgt
     5641 agtagtggtg tttactatgg gcttattctg gaaccagatt acaagcaaag cagccatctg
     5701 gaccgcggcg gctaccattc ctattggaat tgctatcaaa tttgcattac cggaaattcc
     5761 atggattcta cgaatgggtt acgtattcat aatcctgtgt acggtagcct cggttatttc
     5821 attccttgat aaaggagtaa aagtgaaatc accaaccctt ggtgaaggtg ttcagaagaa
     5881 actcatttcc accagttacc tgctaactgc cctcagcatt gtttgcattt tgcctggttt
     5941 gtttttctcc ggtacatatg cgcaccttgg ctttgaatct atatttgtgc ttgcctcaat
     6001 atttcttatg cttgctatta tttcttatac caatgcaaaa attgatgttg aagaccggaa
     6061 ggcgtttcat acggaagtta caatattcaa gaccggagtt ggctttaatg taggtgcaat
     6121 tggtattata gccattgttg cattcctcta tattgtcttc tggatgtaat aaattcaaga
     6181 tgttaccgaa tcttaaaaag cttgaagttt ggtttctgac aggcagtcag catttatatg
     6241 gagatgatgt attgaaacag gttgccgctc attcaagaga gataatttct tcaatcaatt
     6301 tatcaggaca gattccggtt aagattattg ccaggcaaac attaactaca ccggaattaa
     6361 tttatcaggt ttgtctggaa gcgaacagtt cttctggctg tatcggggta atactttgga
     6421 tgcatacctt ttcgccggct aaaatgtgga tcagaggtct gaaaattctt cagaaaccat
     6481 tattacattt tcacactcaa ttcaatgcag aaattccatg ggaagttatt gatatggatt
     6541 ttatgaatct gaaccagagt gcccatggtg atcgtgaatt tgcccatata tgtgcgcgac
     6601 tcaatctgaa gcggaagatt atagccggcc actggaaaga taagtcttgc ctgaagcaaa
     6661 ttggcatctg gacaagagcg gcgattgccc ggtatgattt agatggcgcc cggataggaa
     6721 gacttggtga taatatgcgg tatgtggcag taactgaagg agacaaagtt gaagcggaga
     6781 taaaactcgg attttcaaca aatggatatg gaattagcga attggctgaa ttgattacaa
     6841 aaattactaa tgcccgggcc gaaaaacttg tgtacgaata tctggatgaa taccaggttg
     6901 tccgggagct taaaaaaggc ggcccgaagt attcttcact ggtttatgca gcaagaattg
     6961 aactcgggct gcggacattt ctgaaaaaag aaaacctggt tggttttact gacacctttg
     7021 aagatttgca cggactggaa caactgccgg gaattgcggt tcagaggctg atgcgtgatg
     7081 gatatggctt cggtgctgaa ggagattgga aaaccgcagc cctggtaaga gcgatgaaag
     7141 tgatgagttg cggtcttgaa ggaggtaatt cctttatgga ggattatacg tataatttca
     7201 cttcagtgga aaatcaggtt ctgggagcac acatgctcga aatatgtgaa tctttagcgg
     7261 agtctaaacc ttcttgcgag attcatcccc tgagtattgg aggcaagtca gatccggtca
     7321 ggcttgtatt taatgttgga gcgggaaagg ctgtaaatgc atcccttatc gataccggca
     7381 gcaggtttag aatgttggtt aataaagttg aactcaggaa gcctgaaaag gcattgccaa
     7441 agttgcctgt agcgagagct gtttggaaac ctcttcctga cctaaaaaca tccgctaccg
     7501 cctggctgat ggctggaggt tcacatcaca cctgtctgag taaagcattg gaggtggagc
     7561 atctgatgga tttttgtgaa atgatgaaca tagaattact tgtgattgat gagtccacga
     7621 acctggtaaa tttccataat gaattaagat ggaatgataa atcgttcgaa gaaaaataat
     7681 ttcctctaat tctgaaattc catatcatca ttcccaccat tgtcatcatt atcttcacca
     7741 ttatctttgc gcttctgatt tatgcggtag gtgaaagtaa tgcgcaattg gcgtgatctc
     7801 cactgaaatt cagattctga gtaaaaatat tgcccttcag aaattccacg gtatttcctg
     7861 gtattgaaaa ggtcgcggat attcaaactt atggtaccat tacctttcag gatcagccag
     7921 ttggctccaa tgtctgcatg atattggctc ttcctcctgc cctgggtagt attttccgga
     7981 gccctgtatc ttcctctaag ctgtaggtca actttaccca gatcgaactt tgttgaaaga
     8041 ttaaattggg tagtataggt ttcactgctg agacgttcgc cattaaaatc tccatcaact
     8101 ttggcatgat agaaatttgc atttccattc aggtttagcc actccagcgg atcgctggag
     8161 acgtttagtt caattccgta tgattcttcc gatccaaggt tatgaggttg cgaataagtg
     8221 gtaccattag tttcgacata atttatccgc tgaatcatac ctgaagtata ccggtaataa
     8281 atgccaccgt acaaactagt tttttccctg ttgttcaaat atcctgcttc gtaggagtta
     8341 gtgtattccg gatccaggtt tggatttccg gtccggatat tcctgggatc attgaatgat
     8401 aaaaatggat tcaggaacca atgacgcggc cttgatagtc tgcggctgta acttacttgt
     8461 atggtattta attcagagag cttgtaggtc aggtaaacgc tgggaaagaa attcaaataa
     8521 gactttttac tttcttcagc cgtttcacgc tgataagttt ttatccttgt attttccgcc
     8581 cgcaatccca cctggtatcc ccacttgggt aatttatgct cgaaaatggc gtagcctgca
     8641 tttacatcct catcatatat aaattgattg gagaaattat caagggagat ccaatttccg
     8701 gaggtattct gttcttccac caggtaatca ctctccattt gccgaaatgt gcctctatag
     8761 cctgcttcaa atttaaatgc ctcattcacc ggatatacat aatcaagttg agccagccaa
     8821 ttctgatctc cttccctgtt cgatgagcgt tggaataaat atggcgcatt attggtcaat
     8881 gcatcggatt cactgatttg tgatttctca gtttctgaat tgtctctaaa ctgaacttcc
     8941 gcggccagct catgtccatc gccgggcaat gtttttcgat aaaccagttg aaattccgag
     9001 ttttgatcat cttcagtttc attgtccagc cttaatgttt tatccaacaa tactccatca
     9061 ccggaataat catcataaat aatgcggctg tcattgcttt catcagaaat tctatataat
     9121 cctgaagctg tgataacgtc gttatcggtg atatatgtgt caatcccgaa tctgaaagaa
     9181 tttccaaaat caccgcgctc gcgatcctga tcgcggtagg taacataagt tgtatcaggg
     9241 aaggtaaact tctgatacga cgatccgcca ccaggacgtt tgtcataatc gataccgtaa
     9301 ttggcgaaga aattaaattt tcctacttta taattaaaat tagtagaaat accaacatca
     9361 acaggtaatc cgccatttat attaaaagaa ccattgaaac ccatacgctc ctgttttttc
     9421 agaataatgt taatgatacc tgcggttcct tcggcatcat accgggccga gggatttgtg
     9481 ataacttcaa cactttctat gaggtcaccc tgcagcatcc gcaaagcatc agggccgcta
     9541 atcccaacaa gcccggatgg ctttccatca accagaattc tcacattttg atttccccgg
     9601 aggcttacat tgccttctac atccacgttt atggaaggca ggttatctaa aatatccgct
     9661 gcattaaggg atacattatt tatattctgc cccacattaa aaatccgctt atccaattcc
     9721 aatctcattt gatttttctc accggtgacc actacttctt ccagtaactt cacagcttgc
     9781 cgcatcgtaa tggtacccaa tgatctagat tctccatccg ccacggttac caatatcctg
     9841 gaaagctcgt aagaaatgga ctgaatttcc aggtaatatt gtgcaggttc cacttccagc
     9901 ctgaaagctc catcaaaatc cgttatcgct ccatccacca aagtagaatc ggttctatat
     9961 acggccacag ttgtatatgg aagagtttct tttgtgttta tatctattaa cgttccggta
    10021 ataaccgccg atttctcctg cccaaaggca gtgttggcca acattaatac caaaatcaat
    10081 tttaatacta tcgctattcc tattctcact atcttaaatt tttacttatc taacaaacca
    10141 tattcttgca accttattct tgctattgtc tcattaacaa aaataaagtg ggaatttgaa
    10201 gcaaatttga agtgttcgcc cttaagattt tggtaatgcc atggagcctt ttacgagtgc
    10261 cattctttgc tggcaaaagt ggaagtgtgg cacacgtttg atgttacttt tatttacttc
    10321 ttcaagtatt ccagtagagc ctttttacgt gtgccacact ttgccggcaa taggggaagt
    10381 gtggacatac gttgatgttt actttcattt actcttcaag tattccgtta gagtcttttt
    10441 acgtgcgcca cactttgccg gcaatagtgg aacgcggaca cacgctagat gttaatttaa
    10501 tttggattta aactgagaca ctatcaaaat tttacagtca gaacgtgttt gttatcagtg
    10561 tagttatacg agatctggaa attgttggct tcacaaattt tttgaacaat ggcaagtccg
    10621 agtcccagtg agtctccact ttgatcttcc tttctgaaac gttcaaagaa taaggcggga
    10681 tctttttttg gaacatttcc cgtattttca atggttaggg attctgcatt taatgaaacc
    10741 cggattttac ctttttctat gttatgcttt atcgcgttct ggagcaggtt tgcaattata
    10801 acatccatca aaacctgatc agagtgaact ttgacattgc catcaatatt cttgctgagt
    10861 gtaagtcctt tgatttcaat aagttcagtg aaatcatcca gagttttttc cactaatgca
    10921 gacatattca cttcttcaag tttattaaat tcctggtttt caatttttgt taataatgcc
    10981 agcgaatggc ccagcctgct caattttgtt aatgcttcct gggccgaaag aatttgcttt
    11041 agctcgttat cattaagatt tggagaatct atcaacagtt gcagtttact attcattatg
    11101 gccaggggag tttgcatttc atgtgaggca ttctctgtaa attctttcaa tgattggtaa
    11161 tccatcttca ctttcctggt catttcagaa ataaactcat taagctgagt gaattccctg
    11221 gtagaagtct tccgcagatc aaggggatca gatttactca gactaaatcc actaatgaat
    11281 tctaaagttt tattgaaggg tttgaacact ctcgcggaaa aaatgaaagt tattataatt
    11341 acagccacaa ataagatcgc gtagattccc aacatagatt tatatacacc ttcaataata
    11401 tcttccgcct caacaattac gtcatacatt tcaaatcgat agaatgtccc gctgacttcg
    11461 gttatagtct tgagcttgac gtgtggttca agtcttcgca aggttgcgtg gatcaccacc
    11521 gtatcggtga attcaattct ctcttcgccc actccctgag gaactcttgt aatggatact
    11581 ttctcatgat tcaatgattc gattgggact cccttttcaa ttgaattttt cagataaccc
    11641 agttgctctc tcaaatgtct tttttgctcc aaatttacct gggtttgaat gttaacgtat
    11701 gtaattatac ccccggctat aattacaccc agtgttatca acaaatacag tattactatt
    11761 ttgtagatta atctcattca attgtgcttt tgaatttata cccggcaccg taccgggtag
    11821 taatataatc ccggcagcca tgtgctgtta gtttttttcg cagatttttg atatgcgtgt
    11881 aaacaaaatc aaatgaatca ttaaggtcca tatagtcgcc ccaaaggtgt tcggcaattg
    11941 tctgacgggt aattattctt tctttgtttg aaagaaaaaa taataaaagt tcatattctt
    12001 ttcgggtaag ggttactggt tttccatgaa ctttgatttc ttcctttact gtatcaatcc
    12061 gcatttcttt gaattgtatt tctgagtggc catcaaattt tttcctcctg tttatcgcgt
    12121 tcaggcgggc attcagctcg gtgagttgga acggttttgc caaataatca tccgcgccca
    12181 aattcaagcc ttttatctta tctccggtag aattttttgc agataaaatc agtacgccgc
    12241 attgctttcg ttcttctttt atagttttca gaatatccaa accatcacca tccggaagca
    12301 tcaaatccag gatgacaaca tcataatcga aggaaattat cttgtcaatt gcctgtttat
    12361 aattgtaggc attttcacaa acaacacctt ccctggtcat aaaagcctca atgctttctg
    12421 aaagtgattt gttgtcctc
//
LOCUS       DASWHX010000227         1915 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_3243434, whole genome shotgun sequence.
ACCESSION   DASWHX010000227 DASWHX010000000
VERSION     DASWHX010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 1915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1915
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3243434"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1025)
                     /locus_tag="VGA21_14615"
     CDS             complement(<1..1025)
                     /locus_tag="VGA21_14615"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEX9651793.1"
                     /translation="MGSETMKDILLIVWYRFWQLVASLGLKMYFSSLQVNGLKNVPAK
                     GPIILAVNHQNSFLDAIIVACVLNRKSYFLARGDVFRKKWAHTMLSSLGIMPIYRTRD
                     GLGKVKDNTAIFNACSEILKENGAVMIFPEGNHYMRWSLRPLQRGIARIAFQSRESAE
                     IPPIKIVPVGLQYEAHMTAASRILVQIGEPVDVEKYLAMENVDQRTKMDLLLHDLFNA
                     MNPLILDIPEEGYESLVEKLKAVRGYFSNQVQQLQSDQGIISALLNSEPVEVNVRMDV
                     KSRINYLVYPIIPVGWITHILPRLIISTLIRVKVKDPQFIAPLNFALGLLIYPANAIA
                     WGVIITIL"
     gene            complement(1007..>1915)
                     /locus_tag="VGA21_14620"
     CDS             complement(1007..>1915)
                     /locus_tag="VGA21_14620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009185544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HEX9651794.1"
                     /translation="LNSVHLNFSQEKVWMLNLALAFIMFGVALSLETRHFREVARNPK
                     GFWVGVVSQFIFLPAITFLLVYFLRPHPGLALGMILVAACPGGNVSNFYSLVAGGNVA
                     LSVSLTGFATFFAVFLTPLNFEFWGSILPETEEILNSIDISFFEMAKTLVLILIIPLL
                     LGIWFRRKFPPLTIRIEKYVRILSFLILAAFIGFALAGNMDVFSKYIHYVVYLVLLHN
                     ALALITGYLTAKITRLNSRDVKTITIETGIQNSGLGLLIIFTFFDGMGPMALITAWWG
                     IWHLISGIVSAIIFRRFKISQWVLKQ"
BASE COUNT          603 a          450 c          374 g          488 t
ORIGIN      
        1 agaatagtaa taatcactcc ccaggcaatt gcattggccg gataaattaa caaccccagg
       61 gcaaaattaa gtggagctat gaactgggga tctttcacct ttaccctgat aagggtagaa
      121 attattagtc ggggaagaat atgagtgatc catccaaccg ggatgattgg ataaacaaga
      181 taattaatcc ttgatttgac atccattcta acatttactt ctaccggttc gctgttaagc
      241 aaggccgata ttatgccctg gtcagattgt aattgctgaa cctggttaga aaaatatccc
      301 ctgacagctt tcagcttttc aaccaaactt tcatatcctt cctccgggat atctagtatt
      361 agcggattca tagcgttgaa caagtcatgg agcagcaaat ccatttttgt ccgctgatca
      421 acattttcca tagcaagata tttctcaaca tcaaccggtt ccccaatttg aaccaatatc
      481 ctggatgccg ccgtcatgtg agcttcgtat tgcaatccta ccggaacaat tttaataggc
      541 gggatctcag cactctcccg cgattggaaa gcaatccggg caattcctcg ctgcaaaggc
      601 cggagcgacc atctcatgta atgattcccc tcaggaaaaa tcatgaccgc cccattttcc
      661 ttcaaaattt ccgagcacgc attaaaaatg gcagtgttat ctttcacctt tcctaaacca
      721 tcgcgggttc tgtaaatcgg cataattcca aggctcgaca acatcgtatg cgcccacttc
      781 ttcctgaata catcacccct ggcaagaaaa tacgatttgc gattcaacac acacgcaaca
      841 atgatggcat ccaaaaatga attctgatga ttaacggcaa gaatgatggg tcctttggca
      901 ggtacattct tgagtccgtt tacctgcagg ctggaaaaat acattttcaa tcccagggac
      961 gcgacaagtt gccaaaatct ataccatact ataagcagaa tatctttcat tgtttcagaa
     1021 cccattggct gattttaaat cgccggaaga taattgctga tacaatcccg gagattaagt
     1081 gccagatacc ccaccaggct gtgatcagcg ccatgggtcc cattccatca aaaaaagtaa
     1141 aaataataag cagtccaagc cctgaatttt gaattccggt ttcaatagtt attgttttta
     1201 catctcttga gttgagcctg gtaatttttg cagtcagata accggttatc aacgccaaag
     1261 cattatgaag caaaaccaga taaactacat aatgtatata ttttgagaat acatccatgt
     1321 tgccagccaa tgcaaaacca ataaaggcag ccaatattaa gaaagaaaga atccgaacgt
     1381 atttctcaat ccggattgtg aggggcggga attttcgccg aaaccaaatc ccaagcaaca
     1441 atggaattat gaggatgagc accagggttt ttgccatctc gaagaaacta atatcaattg
     1501 agttcagaat ttcttctgtc tccggtagga tgcttcccca gaattcaaaa ttcagcgggg
     1561 ttaggaagac cgcaaaaaaa gttgcaaatc ctgtcagact cacactcagg gctacatttc
     1621 ctccggcgac caaagaataa aaattggaaa cgttcccgcc aggacaagca gccaccagga
     1681 tcatgcccag tgccagtccg ggatgcggcc ttaaaaaata aacgagcaga aaggtaatag
     1741 ccggaagaaa aataaactgc gacaccactc ctacccaaaa tcctttggga tttcttgcaa
     1801 cttccctgaa gtgccgggtc tcaagactta aggccacacc aaacatgata aaggcaagag
     1861 ctagattcag catccatacc ttttcctgac tgaaattcag gtgaacagag ttgag
//
LOCUS       DASWHX010000228        10214 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_3243795, whole genome shotgun sequence.
ACCESSION   DASWHX010000228 DASWHX010000000
VERSION     DASWHX010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 10214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10214
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3243795"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1802)
                     /locus_tag="VGA21_14625"
     CDS             complement(<1..1802)
                     /locus_tag="VGA21_14625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651795.1"
                     /translation="MKSVRLIFLGFIILMLLVCSLAEAANENLSLACTADAGNDVSLC
                     LGGRTTLGGLQAASGGTGPYTYEWSPVTGLDNPFIANPAASPKETITYSLTITDAEGC
                     QSTSAVTITINTPPTANGGSNFEICQGDEVRLGGKPSAMGGQPPYSYQWVPTVGLDNP
                     TKADPIASPTVSSYYFLLVEDAFGCQDVDIVWVEVMDPVALSAGNDQDICSSTASILL
                     NGSVAGATTIEWSTDGTGYFSPGADNLEASYVPSTADKAFTEITLTLTASNASCDPVQ
                     DEVKIFFYPETVVDAGSDTTICSENPDLVLNGSISGSSTVGYWSTSGTGIFDPDSSSL
                     NAKYFPSPEDLLNGSVILTLSSLNSSVCQDSSDSMIITYQKLTADAGSDQEICANETS
                     IAVTGSITGLTDSVFWSSDGFGSFESPNQLITSYIIGKNDLEMDSVTLTLTHVGICSG
                     VTDQVTIFIKKLPEISAGTDFTVCSSGQIELNGTAAGNFAEAFWVSTGDGNFSPDNTG
                     LEAAYVSGSGDIAAGYVDLKLIVNSSNGCNSVEDGIRISFNEEVLVEAGPNQQICADT
                     KFVTLEPAFSAPVDSIKWLATGDGAFTVYPITGNA"
     gene            2033..4093
                     /locus_tag="VGA21_14630"
     CDS             2033..4093
                     /locus_tag="VGA21_14630"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HEX9651796.1"
                     /translation="MRTFTNLFILTGLILPHFLDAQSVTVENFSNYGLTNQGIYLEQD
                     KQFGSFLYEFKLEQQKVDTVGFAAAIGIDGYLQVGGFEPVTEFKIVNKEKTKFSFNGL
                     FGSVLNDTPAEILVSGYKNGMQVAEEVLITINKTKPGKFYDFTANSQFYNVDEIRFRS
                     ENNMSFIIEHWSYSEKCLAAIADAGENSEVCASGSILTANNPSVGTGTWQVIKGSGIL
                     DNPGSFQTQVENLSVGLNEFEWKVVNGYCDSAVSNVQVYRYAEPNLAETVVDFETCST
                     EVTLNANSPTNGTGMWTIIAGTAIINSVNEPITKAGNLSTGENIFVWQISNGICASTA
                     DTLKITKNPSPDESWAGTDFITCSSEVQLAANLPLTGTGEWSVKEGNAIISDKGNPTA
                     YAFNLSEGENIFQWNITNECNTFSSRITITVPFNVTQANAGSNQEVCINEIAMNANVA
                     VIGTGEWSIISGSGQIQDPGNPASLITGLNEGQNLFVWKIKGQECRISSDTVSVTVNP
                     IIVNAGEDKEIFTGQSVTIGPESFDLASGTVFWEGGYEIDDPYLLNPRVSPLETTTYT
                     MTYTDGSGCEGRNSVVVKVIERETIVDLHIPKGISPNNDGINDTWHIRGIEEFSNNAV
                     KIFNRWGQIVFERNGYDNTDPFIGVSKVGLSFGSELPEGTYFYHISLYETNTVFKGYI
                     EVRK"
     gene            4118..4408
                     /locus_tag="VGA21_14635"
     CDS             4118..4408
                     /locus_tag="VGA21_14635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651797.1"
                     /translation="MTIRKFIQKTLKLQSNTELQEVQRLINATENYISSINENSTNKL
                     ERNHELIFLSGIIQHLENNYTQIPDCIYEKILKCNSGVKKKSFENFRKEYSC"
     gene            complement(4632..5534)
                     /gene="floA"
                     /locus_tag="VGA21_14640"
     CDS             complement(4632..5534)
                     /gene="floA"
                     /locus_tag="VGA21_14640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017733738.1"
                     /note="flotillin-like protein involved in membrane lipid
                     rafts; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin-like protein FloA"
                     /protein_id="HEX9651798.1"
                     /translation="MEYALYIWIGAILGGFWVIMYFIPIGLWISATLSGVRVNLFTLM
                     IMRFRKVPPLLIVQSLILSTKAGIKGITTDVLEMHFLARGNVIAVIKALIIADKANLD
                     LSFKQATAIDLAGRDVLKAVGISVTPFVIVVPSIIGVSIEGIQLLVDVRVTVRANIKQ
                     LVGGAGEETIKARVGQGIISAIGKAKNYKAILGNPEFISKEVLMNGLDSGTAFDILSI
                     DIADIEVGKNIGAALQIDQAKADLHIAQAKAEERRAVAVALEQEMIAKSQEARALVIE
                     ALAKIPMAMAGAFRRGSMYANDQN"
     gene            complement(5551..5673)
                     /locus_tag="VGA21_14645"
     CDS             complement(5551..5673)
                     /locus_tag="VGA21_14645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651799.1"
                     /translation="MSKEKGGKEQSVKKKPAKNLKEKRAAKAAKRMEKLEIRKI"
     gene            5982..6542
                     /locus_tag="VGA21_14650"
     CDS             5982..6542
                     /locus_tag="VGA21_14650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018475781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX9651800.1"
                     /translation="MKLYIKFMVSLRCKLMVKEELNKLGVKNAIIDLGVVEIFEDITK
                     EQHKLLKKNLLKSGLELLDDKRSILIEKIKNVVVEMIHYSDELPKENFSDYISNKLGF
                     DYTYLANTFSEVKGITLQHFIIINKVEKVKELLMYNDLNLTEISYRLNYSSVAHLSNQ
                     FKKITGTTPSYYKQLKLKRRSNLESL"
     gene            6871..7221
                     /locus_tag="VGA21_14655"
     CDS             6871..7221
                     /locus_tag="VGA21_14655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651801.1"
                     /translation="MEIKGTELEKCKVYITANVIDYLPDSIITRTILNKNSGNVKVTA
                     IDSSKVLKEVSPFDSLIQIIEGKAEIVIDELSILVEAGQFIIVPAHSHNFIRATERFK
                     MFSTVIKSGYEDVV"
     gene            7255..7476
                     /locus_tag="VGA21_14660"
     CDS             7255..7476
                     /locus_tag="VGA21_14660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018477601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651802.1"
                     /translation="MRSSIIDNKFKEGEVVCARVDPGQRLVIRRYNDRIYYCKIQASP
                     GTNELVYFERELMPPAENKASGEKTQIIK"
     gene            7509..8606
                     /locus_tag="VGA21_14665"
     CDS             7509..8606
                     /locus_tag="VGA21_14665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019600036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HEX9651803.1"
                     /translation="MEQLKSNHNISSVIIGSGSYIPTRRITNQDFINSAFFDETGKRL
                     TKPNAEVIKKFGEITGIQERRYVSEDLVASDIAYLAAKDAIETSGINKETLDYIIVAH
                     NFGDIKEENRRSEFVPALASRVKHHLNIKNSNTICYDLPFGCAGWLQAFIQSDFYLKS
                     GSAGRILVIGAETLSRISDPHDRDSMIYADGAGAVIVEGKKGSKPAGILSHCSHTYTT
                     DHAYALRMGKSNNPGYSLDNLFLKMQGRSLYEHALKKVPKVIKESIDKAGLSLSQIHK
                     VLIHQANLKMIEAILRRLFEEYGIKKIPDHVFPLTIGWLGNSSVATLPTLYDLFSKDK
                     LQDHKFEHGSNLVFASVGAGVNINSMVYRVP"
     gene            8721..9599
                     /locus_tag="VGA21_14670"
     CDS             8721..9599
                     /locus_tag="VGA21_14670"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010611626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HEX9651804.1"
                     /translation="MEKEKLTLEQVINLGTPDVIRYAVPVMIILVLAEWGISYYQKKE
                     IYNKRDLLAATLIGTGNLIVSALIKTSLFVTFLFIINLMPWSIPPTWWSYILCIIAID
                     FFRYWGHRVAHENRFWWATHVTHHNSKKYNWSVSFRLSWVQHIKFVFFIPVAMMGFHP
                     VVFFICHQIEVLYQFWIHTEYIRKLPKPIEYIFTTPSHHRVHHATNAKYIDKNYGSTL
                     IIWDRMFGTFQPEEEQAIYGITKPLTTYNPITLVFHEWRDICKDLYRAKNIKEARKYL
                     FGRPSELSKQESESAE"
BASE COUNT         3236 a         1982 c         2049 g         2947 t
ORIGIN      
        1 gcatttcctg taatagggta tactgtaaat gctccatcac cggtggctag ccatttaata
       61 gaatcgacgg gtgcggagaa agctggttct aaggtgacaa attttgtatc ggcacaaatc
      121 tgctggttcg ggccggcttc caccaagact tcttcattaa aggaaatcct tatcccatct
      181 tcaacggaat tacatccatt tgaagaattg acaatcaatt taagatcgac atatccggcc
      241 gcaatgtcac ctgaaccgga aacgtaggct gcttccaatc cggtattatc agggctgaaa
      301 tttccatcgc cggttgatac ccagaatgct tctgcaaaat ttcccgcagc cgttccgttt
      361 aattcaattt gcccggaact gcaaactgta aaatctgtcc ctgccgagat ttccggcaat
      421 ttcttgatga aaatagtaac ctgatccgtg accccactac aaatacccac atgagttaat
      481 gttaaagtta cgctgtccat ttcgaggtcg tttttaccaa ttatataact ggtaatcaat
      541 tgattaggac tttcaaagct cccaaatcca tcggatgacc aaaaaacgga gtcggttaaa
      601 ccggtaattg atccggtaac cgcgattgac gtctcgttag cacaaatctc ctggtcacta
      661 ccggcatcag cagtaagttt ttgataggtt attatcattg aatcagaaga atcctgacac
      721 acagaactat ttaaggaaga taaagtaagg ataaccgaac cattgaggag gtcttcgggg
      781 ctggggaaat actttgcgtt caaactggaa gagtccggat caaagatacc ggttccggat
      841 gttgaccagt aacctacagt ggaacttcct gaaatggaac cgttcaaaac caaatcagga
      901 ttttcagaac aaattgttgt atcacttccg gcgtccacaa cagtttcagg gtaaaagaaa
      961 atctttactt catcctgaac cggatcgcag gaagcattgc ttgctgtgag ggtgagcgta
     1021 atttctgtaa atgctttatc tgcagttgag gggacataag aagcttccag gttatccgct
     1081 ccgggtgaaa aatacccggt tccatctgta gaccactcga tcgtggttgc accggcaaca
     1141 cttccattta ataatatact ggctgtgctg ctacaaatat cctgatcatt gcctgctgac
     1201 aacgcaacag gatccataac ttcaacccag acaatatcta catcctggca tccaaaggca
     1261 tcctcaacaa gcaaaaagta gtaagaagag accgttggag aagctatggg gtcagctttt
     1321 gtgggattat ctaaacccac agtaggtacc cattgatagg aatagggggg ttggcctccc
     1381 atagcggaag gtttaccccc taacctaacc tcatccccct gacaaatttc gaaattagat
     1441 ccaccgttgg ctgtcggtgg tgtattgatt gttatggtaa cggctgaggt agattgacac
     1501 ccctccgcat cagtaattgt taatgaataa gtgattgttt ctttagggct ggcggcagga
     1561 tttgcgataa agggattatc taatcccgta accggactcc actcgtacgt ataaggacct
     1621 gttcctcctg aggctgcctg gagtccgccc agggtggttc tgcctcctaa acaaaggctt
     1681 acatcgttac cggcatctgc tgtacaagcc agagacaagt tctcattagc ggcttctgcc
     1741 agtgagcaaa ccagaagcat caggataata aaacctaaga agattaaacg tactgacttc
     1801 ataaacttga tttaattatt agaccatgca aaggttggca gcacgcaagt attctttatg
     1861 ggaaaattca ggaatgaaat tctttaggca ttttacctat gcttagctct ggagtctcaa
     1921 ttgagttttt aagaaatggg taaaatacct atttcctcca attcaggtat tctgtaatat
     1981 ttaaatgaag cctatcaatc atctttgggg tgttatttta aataaaatgt tgatgcgaac
     2041 ctttacaaac cttttcattt taaccgggct gatactaccc cattttcttg atgcccaatc
     2101 tgttactgtt gagaatttca gtaattacgg tttaaccaat caaggaattt acctggaaca
     2161 agacaagcaa ttcggttcct ttctttatga atttaaattg gaacaacaga aggtggatac
     2221 tgtgggattc gccgccgcaa ttggtataga tggatacttg caagttggag gattcgaacc
     2281 tgtaactgaa tttaaaatag tgaacaaaga gaaaacgaag ttctcattta atggattatt
     2341 tggttcagta ctgaatgaca caccggcaga gattttagtt tcaggatata aaaatgggat
     2401 gcaggtagcc gaagaggtcc tgataaccat aaacaaaaca aagccgggta aattttacga
     2461 tttcacagcc aattcgcaat tttataatgt agatgaaata agattcagaa gtgaaaataa
     2521 tatgtctttc attattgagc attggtctta cagtgaaaaa tgcctggctg caatagcgga
     2581 tgccggagaa aatagtgaag tctgtgcttc aggcagcata ctaacagcga acaacccctc
     2641 agtcgggaca ggaacctggc aggtaatcaa agggtcagga atacttgata atcccggttc
     2701 atttcaaacc caggttgaaa acctaagcgt tggcttaaat gaatttgaat ggaaagttgt
     2761 gaatggatat tgcgacagtg cagttagcaa tgtgcaggtt tacaggtacg ccgagcctaa
     2821 tctcgcggaa acagtagttg attttgaaac ctgttcgact gaagtcactc tgaatgccaa
     2881 ttcaccaacg aatggtaccg gaatgtggac cattatcgcc gggacagcaa taattaattc
     2941 tgtaaacgag cctattacga aggccggtaa tctttcaacc ggtgaaaata tttttgtatg
     3001 gcaaatttcc aatggtattt gtgcttccac agcagacaca ttaaaaataa caaaaaaccc
     3061 aagccctgat gagtcatggg caggaactga ctttatcact tgttcttctg aagtgcaact
     3121 tgcagccaac ttaccactaa ccggtacagg tgaatggtcg gtgaaagagg ggaatgcgat
     3181 tatttcagat aaaggaaatc ctaccgcgta tgcttttaat ttaagtgaag gtgaaaatat
     3241 ctttcagtgg aacattacca atgaatgcaa tactttttca tctcgcataa ctatcacagt
     3301 tccatttaat gttacccagg caaatgccgg aagtaaccag gaagtgtgca tcaacgaaat
     3361 cgcaatgaat gctaatgtcg cggtgattgg aaccggcgag tggagtatta ttagcggttc
     3421 gggacaaatt caggaccccg ggaatccggc gtctctgata acgggcttga atgaaggaca
     3481 aaatttattc gtgtggaaaa tcaaaggtca ggaatgccgg attagttccg atacggtatc
     3541 ggtaacagtt aatccaatta tagtgaatgc cggggaagat aaagaaatct ttaccgggca
     3601 atctgtaacc atcggtcccg aaagttttga tcttgcttca ggaacagttt tttgggaggg
     3661 agggtatgaa attgatgatc cttatttatt aaatcccagg gtcagcccgc ttgaaaccac
     3721 cacatatact atgacctata cggatggtag cggctgcgag ggtaggaatt cagttgttgt
     3781 taaagtgatt gaaagagaaa ccatcgtgga tctgcatatt ccaaagggaa tttcaccaaa
     3841 caatgatggt atcaatgaca cctggcacat caggggtatt gaggagtttt ccaataacgc
     3901 ggtgaagata tttaatcgct ggggacagat tgtgtttgaa agaaatggat atgacaatac
     3961 agatcctttc atcggagttt ccaaagtcgg tttatccttt ggatccgagt tgcctgaagg
     4021 cacgtatttt taccacataa gtttgtatga aacaaataca gtattcaaag gttatattga
     4081 agttagaaaa taattcttac ctctcacata gaataaaatg actatcagaa aattcatcca
     4141 aaagacctta aaattgcaat ctaatacaga gttgcaggaa gttcagagat taattaatgc
     4201 aactgagaat tatatttcat caataaatga aaattccacg aataagctgg aaaggaatca
     4261 tgaattaatt tttctaagcg gaattattca acaccttgag aacaactata cccaaattcc
     4321 ggattgtatt tatgagaaaa tattaaaatg caatagcgga gtcaaaaaga aatcatttga
     4381 gaatttcaga aaagaataca gttgttagcc tggaaattgt cccccttgtt ttaaggggga
     4441 ctaaaggggg ttttccttcc cacatataac aactgcattt ctattttcaa aactacccct
     4501 gggaaaaata ttaaccaccc ctgccccctc cttaaaataa ggaggggaaa ttaccccctg
     4561 cccagaaatt gtcccccttg ttttaagggg gactacaggg ggtttttgaa tttcagattt
     4621 tgctaatgaa attagttttg gtcgttcgca tacattgagc ctctcctgaa tgcaccggcc
     4681 atggccatgg gaatctttgc caatgcttca ataaccaaag cacgggcttc ctgtgacttg
     4741 gctatcattt cttgctccag ggcaaccgcc actgctcgtc tttcctccgc tttagcctgc
     4801 gcaatatgta aatctgcctt ggcctgatca atttgtaacg cggctccaat attttttcct
     4861 acctcaatat ctgctatatc aatggaaagg atatcaaaag cggtgcctga gtccaatccg
     4921 ttcataagga cttctttgga aatgaattcc gggttaccaa gaattgcttt gtagttcttt
     4981 gctttgccga ttgccgaaat tatgccctgg ccaactctgg cttttatcgt ttcttcacct
     5041 gcgccgccaa ccagttgttt aatgttggct cttacggtaa ctcgcacatc caccagcagc
     5101 tgaatgcctt caatggatac cccgataata gagggtacga caataacgaa cggagtcaca
     5161 gaaataccaa cggctttcaa tacatctctg cctgccaggt caatggcagt agcctgcttg
     5221 aaggaaaggt caagattggc tttatcggca ataatcaatg cctttatcac tgcaatgaca
     5281 ttacccctgg ccaaaaaatg catttcaaga acatcggtgg taattccttt aatgcccgct
     5341 ttggtagaaa gaattagcga ttgaacaata agaagcggcg gcactttacg aaaccgcata
     5401 atcataagag taaacaaatt aacccttaca cccgacagag ttgcgctgat ccacagacct
     5461 atggggatga aatacataat cacccaaaat ccacccagga ttgcaccaat ccagatgtaa
     5521 agagcatatt ccatcattgt ttttagtgac ttatattttc cttatttcca acttctccat
     5581 tcgctttgca gcttttgcag ctctcttttc ctttaaattt ttggcaggct ttttcttaac
     5641 ggattgttcc ttccctccct tttcttttga catttctttc tattttatat gatactttaa
     5701 actgatgata atgtaataat ataattagca atggtaaagg taaatcactg atacttttat
     5761 gacattacat tcttatgtaa gaacgttaca taaatcacat gttttaccct gaattttctc
     5821 attaccggat gatttgatag cacacttaaa attacccggg taaaatttaa aaattctttt
     5881 cattgcctta tttttgctat cggtcttatg ggccaaaaat acattatcct tcatatatta
     5941 tccttattta cggagcacgg aatgtttgaa taccattcaa aatgaaactc tacattaaat
     6001 ttatggtcag tttgcgatgc aaattgatgg ttaaagaaga acttaataaa cttggggtga
     6061 agaatgcaat tatagacctc ggggttgttg aaatttttga ggacataacc aaagaacaac
     6121 ataaactttt gaagaaaaat ctactcaagt cggggttaga gcttctggac gataaaagga
     6181 gtattttgat tgaaaagata aagaatgttg ttgttgaaat gatccattat tccgatgaat
     6241 tgcctaaaga gaatttttcg gactacataa gtaataaatt aggtttcgac tacacttacc
     6301 ttgccaatac attttctgag gtcaagggaa ttacattgca gcatttcatc attataaata
     6361 aagttgaaaa agtaaaagaa ttattaatgt ataacgacct taatcttacc gagatttcct
     6421 atagattaaa ttatagtagc gtcgcccatt tatctaacca gtttaaaaaa attacaggca
     6481 ccacaccttc ttattataag caattaaaac tcaaaagaag aagtaaccta gaaagcctgt
     6541 gagttaggta atcagcttgg agaataatct cttccttttc attaatcctt gatatcagtt
     6601 tcttccaaat acaaatttgg actaataatg aatgccagtc cgcattcgtt tgaatgagtg
     6661 gctcacgctt gagaaataca ctaaaacaag tttgaatggc attacagacg cctcaattcc
     6721 aggaaaactg tgatatatat aaagattatt caaaaaaatg taacattctc caaacccata
     6781 ttcataattt tgtttagaca agattttata ttcaggaaaa tatttatgca aaggaagtgc
     6841 tgaattacaa tttcaagaat cttaggatta atggaaataa aaggaacgga actcgaaaag
     6901 tgcaaggttt atataaccgc gaatgtaatt gattacttac cagactcaat tatcacaaga
     6961 acaattctaa ataaaaactc tggaaatgtt aaagtgactg caattgattc cagcaaggta
     7021 ttaaaggaag tctcaccatt tgattctctt attcagatta ttgaaggaaa agcagaaata
     7081 gtgattgatg agttatctat cctggttgaa gccggtcaat tcataattgt ccctgcccat
     7141 tctcacaatt tcattagggc gacggaacga ttcaagatgt tttctaccgt tattaagagt
     7201 ggatatgaag atgtagtttg atttgaatta ttgtgaattc aaaattgaca caaattgaga
     7261 agttcaataa tcgataacaa atttaaggaa ggcgaagttg tttgtgctag agtagacccc
     7321 ggccagaggt tggttatcag gcgctataac gacaggatat attattgtaa gattcaggcg
     7381 agcccgggca caaatgagct tgtctatttt gaaagagagc taatgcctcc tgcagaaaac
     7441 aaggcatccg gcgaaaaaac tcagattatt aagtaaacaa gtgatagtaa ataattatcc
     7501 ataattccat ggaacagcta aaatcaaatc ataatatttc ttccgtaatc attggaagcg
     7561 gaagctatat acccacgcgt agaatcacca atcaggattt cataaattct gcattttttg
     7621 atgagacagg aaaaaggctc accaaaccaa atgcggaagt cattaaaaaa tttggcgaaa
     7681 taaccggcat tcaggaaaga aggtatgtaa gtgaagatct tgtagcatca gatatagcat
     7741 atcttgcggc aaaggatgcc attgaaactt caggcattaa caaggaaaca ttagattata
     7801 taatagttgc gcataacttt ggagatataa aagaagaaaa cagaagaagt gagttcgtgc
     7861 ctgcgctggc atcaagagtt aaacaccatc ttaatattaa aaattcgaat acaatctgtt
     7921 atgatttgcc atttggatgt gcaggctggc ttcaggcttt tattcaatct gatttctacc
     7981 tcaagtccgg ttcagccgga agaatactcg ttataggggc tgaaaccctc tcacgaattt
     8041 cggatccaca tgaccgcgac agcatgattt atgctgatgg agccggtgcc gtaattgtgg
     8101 aagggaagaa agggagtaag ccggcgggaa ttctttcaca ttgctctcat acctacacaa
     8161 ctgatcatgc ctacgcatta agaatgggga agtccaacaa ccccggatat tctctggata
     8221 atctgttttt gaaaatgcag ggtcggtcac tttacgagca tgcacttaaa aaagttccaa
     8281 aagttattaa agaaagtatt gacaaggccg gtttgtctct aagtcaaatc cacaaagtgc
     8341 ttattcatca ggctaatttg aaaatgattg aagcgatttt acgccgcctt tttgaggagt
     8401 atggaatcaa aaagattcct gaccatgttt ttcccctaac catcggatgg ctggggaaca
     8461 gctcggtagc caccttacct actttatatg acttgttcag caaggataaa ttgcaggatc
     8521 ataaatttga acatggaagc aacctggtat ttgcttcggt tggtgccggt gtaaatataa
     8581 attctatggt atatcgggtt ccctgatttt acaggcatta tattcgccaa taaatcaaag
     8641 atttaaatga ttttgaaatt atgtgatgaa agtcggacct ggtagaataa aaataaattt
     8701 acctgggctt aaagtaatta atggaaaaag aaaaactgac acttgagcaa gtaatcaatt
     8761 tgggcacacc ggatgtaatt cgatatgccg tacctgtaat gataatcctg gttttggcag
     8821 aatggggaat atcttattac caaaaaaaag aaatatacaa taagagagat ttgttggccg
     8881 ccactttaat aggcacaggt aacttaatcg taagcgcact gattaagacg agtttgtttg
     8941 taacgttttt gtttataata aatctaatgc cctggtccat tccacccacc tggtggtcct
     9001 acatactttg cataattgct atcgactttt tcagatattg gggtcacagg gtagctcatg
     9061 aaaacaggtt ttggtgggct actcacgtta cgcaccataa ttccaaaaaa tacaactggt
     9121 cagtttcgtt tcgacttagt tgggttcaac atatcaaatt tgtattcttt ataccggttg
     9181 caatgatggg atttcacccc gtagtttttt tcatttgtca tcaaatagaa gttctctatc
     9241 aattctggat acatactgag tatattcgaa agcttccaaa acctattgaa tatatcttca
     9301 ccactccttc ccatcatcgc gtacaccatg ctaccaatgc aaagtacatc gataagaact
     9361 acggctccac attaattatt tgggatagaa tgtttggaac ttttcaacct gaagaagaac
     9421 aggcaattta tggtattacc aagcctttga ctacctacaa cccaataact ttagtgttcc
     9481 atgaatggag agacatatgc aaggatttat acagggctaa aaacattaag gaagcccgca
     9541 aatacttatt tggcaggcct tctgaattat cgaaacagga atcggaatca gcagaatagc
     9601 cattgctgca aaatttaata tttggaatga tgaagcgatc ccctgagctt ctgcgttcaa
     9661 aaactgcttt acacctcctt aatacaattc ttctttgtat ctttgtacat gaatatttta
     9721 aggagctgga gagaacaaaa aattttatta aaaagaagat ttcccatcct tacagatgaa
     9781 gactttcaat ttaaagatgg cgaaaaggaa agtatgctca agaccttaca aaacaaatta
     9841 ggtaaaacta agagcgagct tgagtcgatc tttgctgaga tacaattaac ctaagccaac
     9901 ccaatctagg aaaattgtct ctcttgtttt taaggggaac tgcgaggggg ttttgaatta
     9961 ccgcaaatac atcagctctg tcagaaattg tcccccttgt tttaaggggg actacagggg
    10021 gtttgaacca cccctgccaa gaaaattgtc tcccttgtct taaggggaac ttcaggggtt
    10081 ttgaattacc gcaaatacat cagctctgtc agaaattgcc tcccctgttt taaggggaac
    10141 tgcgaggggg ttttgaatta ccgcaaatac atcagctctg tcagaaattg tcccccttgt
    10201 tttaaggggg acta
//
LOCUS       DASWHX010000229        10959 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_3244077, whole genome shotgun sequence.
ACCESSION   DASWHX010000229 DASWHX010000000
VERSION     DASWHX010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 10959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10959
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3244077"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1428
                     /locus_tag="VGA21_14675"
     CDS             <1..1428
                     /locus_tag="VGA21_14675"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010610765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX9651805.1"
                     /translation="ELNLDLSGVKNYDELVSHVADAVAKANPGDWIVGRGWHQSKWDP
                     VPEEMVDGFQTHKKLSEISPENPVWLSHASGHAGFANARAMEIAGVNPLSIESLEQNV
                     EGGEIITDALGNPTGIFSETAQGLIRRHIPENTPAMDRRALELAIKACQRNGITSFHD
                     AGTGQGTIDLMQQFKEEGILGVRMYIMLAGRSEELINQWLEKGPVHDTDDQLLTIRAI
                     KLNADGALGNRGAWLLEEYTDRQGHFGFATMPLEYMERVTRDGLSAGFQVNTHAIGDR
                     TNREILDIYEKVLTEIPEKATDHRFRIEHAQHLSLDDIPRFGELGVIASMEAIHMSSD
                     RPWAIDRLGEKRIVDGAYVWQKLLQSGAIIVNGTDVPVEPIDPIACFYASVSRKTLAG
                     IPEGGFEPEQKMSREEALRSYTLDAAYGEFAEEVKGSISPGKFADFTVFDQDIMTVPE
                     NQILDTKVTMTVFNGKVVYDGNPSM"
     gene            1441..1977
                     /locus_tag="VGA21_14680"
     CDS             1441..1977
                     /locus_tag="VGA21_14680"
                     /inference="COORDINATES: protein motif:HMM:NF022588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HEX9651806.1"
                     /translation="MKILFTFMVLLIVACTAEKKVGEDSSRPEREISSPRREATGNAS
                     GINIMVDYGSPAVKGRRIWGGLEKYDRVWRAGANETTAISFDKDALINGEPIDKGKYA
                     IFIIPKENEAWIVIFNQDWDEWGAFRYNQERDVLRFTVSPEWSDNIHERLEYSIEEGN
                     LTFRWEKVSLSMAVSSRN"
     gene            2114..2536
                     /locus_tag="VGA21_14685"
     CDS             2114..2536
                     /locus_tag="VGA21_14685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009577459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX9651807.1"
                     /translation="MSKKKVTGIGGIFFKSEDPKKLNEWYEKNLGLVLNEYGSLFEFR
                     MSDNSDTKGYLQWGPFTKDTRYFDPSQKQYMINYRVENLEELVEELKSAGVEVVDEIE
                     IYEYGKFVHIMDPEGNKLELWEPVDSVFTKMYEGKTTK"
     gene            complement(2537..3070)
                     /locus_tag="VGA21_14690"
     CDS             complement(2537..3070)
                     /locus_tag="VGA21_14690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019599578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal-dependent hydrolase"
                     /protein_id="HEX9651808.1"
                     /translation="METPDLRYPIGKFNAPEAFSKDSIDDWINQIEQLPARIKSETEY
                     LGKEELDYRYRPEGWTVRQVIHHLADSHMNSFMRFKLTLTERSPTIKPYLENMWAELP
                     DAKDTPVAHSLNILDGLHKRWVYLLKTLNDDQFNLEFIHPEYNKTYKLFVVTALYSWH
                     SNHHLAHIKQAKIRKEK"
     gene            complement(3156..4052)
                     /locus_tag="VGA21_14695"
     CDS             complement(3156..4052)
                     /locus_tag="VGA21_14695"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEX9651809.1"
                     /translation="MKITFVVNSLYHSQSAFRKNILPQLYLTSNKDIDYLFTEKKGDS
                     ILLTRKAIESGTTLIIGYGGDGTLHEILNGYMTSGSQDKDSIIIGQFPIGTGNDFSRN
                     FNLSKKIADLSRRLHSPSIRKIDIGQLTLADGTNVYFHNEVSFGFSAEVIRKMEISKG
                     TGKKSYFKSIMQAFNSYRPTAVKIVGDNFKWEGKAFMIVVANGKYMGGGMSLAPDASP
                     FSGLFSITIVGEVSMWEYLTQLPYIFQSKKLKHPRVYYFEARDLEIHSGEVTFAAESD
                     GELIAPVPKRIVCLPSRIKFLG"
     gene            complement(4105..5187)
                     /locus_tag="VGA21_14700"
     CDS             complement(4105..5187)
                     /locus_tag="VGA21_14700"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX9651810.1"
                     /translation="MNHFSIHQEISKAETLPAAFYRSDEVFNSFKEKVFAVTWHLVGN
                     EKLFNLKTNLYPINFYPGFMDEPLLLTCDENDSVAALSNVCTHRGNVLINSPAKKSKL
                     ICGYHGRKFALDGKFEFMPEFKEAENFPSNCDNLPKIPLAKWKQFFFLSLNPAYEFIK
                     IEKILNERTGFLPVENFTFKPELSREYLVNSHWALYCDNYLEGFHIPFVHNDLNEAID
                     YGTYETKTFEYGNLQIGYAKGGTEKFDLPEGHIDYGKNVSAYYYWIFPNLMLNFYPWG
                     LSVNIVQPLTKDRCKVIFQIFMHDESKMTNSAGALLDKVEREDEFVVESVQKGVNSML
                     YKAGRFSPKREIGVHHFHLLLSKFLG"
     gene            5254..6564
                     /locus_tag="VGA21_14705"
     CDS             5254..6564
                     /locus_tag="VGA21_14705"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEX9651811.1"
                     /translation="MQIPDAKPKVGFLTATSLVVGNMIGSGIFLLPATLAFYGGISIF
                     GWVISGIGAIFLALVFGRLSKLVPNSGGPYAYTREGFGDFAGFIVSWGYWISIICTNA
                     AIAVTLVSYLSVFIPVIAASNLTSALIAVGTVWFLTWINSLGIKRGGNMQVITTVLKI
                     IPLLAVSIAGIFYLNIDHFQPWNISSQSNIDAVGATVALTFFAFMGMESATIPSEDVK
                     NASIVIPRATITGTMLVLLIYMLSYVALMGLIPPESLQKSTSPFADGAGLIWGDTGRY
                     IIGLGAIISTFGALNGWILLQGQVPYAIARDRLFPHSLSKVNIHKAPVTGLVVSSIFI
                     TILIISNFTKGLVGMFTFMILLTTVVVVFPYLFCSLAEILILIKRKKSKTKFIKPLLV
                     GIPSFLYLMWALTGVGKDEVYYGLLLIIAGIPFYALIKYKTKRE"
     gene            complement(6581..9274)
                     /locus_tag="VGA21_14710"
     CDS             complement(6581..9274)
                     /locus_tag="VGA21_14710"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005388 - P-type calcium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006816 - calcium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HEX9651812.1"
                     /translation="MKYEFKGLSTQEVLDSRKKFGSNELSPYEIKSFWSKLVGNFQDP
                     IIIILTAALVIIFVLSIFELTEWYEALAIAIAVALATLVSTFSEYKNESSFQKLQEEA
                     SRIENTAFRGGQLRKIPVGEIVTGDYVLLQAGDKVPADGKVVKGELRVNQASLTGESD
                     SVYKTKMPFDYLPKAKDLADPHLIFRGSVVDDGEGVVRVDTVGNNTFYGHLAKELAEA
                     EGRLSPLQVKLKGLAQLISRFGYIAAIFIAIAFTFNKIVVANGFEGDKIMAYLSQWEI
                     LIPDLLDALVLSIIIVVAAVPEGLPMMIAIVLSLNMRKLLVEKVLVRKLLGIETAGSL
                     NILFSDKTGTITKGQLEPKLFINSAGTIFPDFNSIPLELKELLGLSVIENSSSYISPE
                     GDVIGGNGSERALIAFVSKNEIEKTMKLGAEGEKKILFNSERKFSATQVKIDYQIKGL
                     DSTRITLIKGAPEVLIPECTHQFEDDGTKVPLTSTDALYKEMDELAEAGIRLIALAVT
                     NELIKDDHSIPNSACLVGIVGIGDEVRAESFRAIKEVQNAGIQVVMITGDKKGTASSV
                     ATEVGLLTRSDDVVMVSSELSEHSDEDIKRVLGNLKIIARALPSDKSRLVRISKSLGK
                     VVGMTGDGVNDSAALKHADVGVAMGSGSEVSKEAGDIVILDDNFHSISNAIRYGRTIF
                     KSIRKFIVFQLTVNVAAVCTAFLGPLIGVDFPLTIIQILWINIIMDTLAAIAFGGEPA
                     LLRYMEEPPVNRDENILTKNMVSAILTSGLFITFFSIFFLTFPPFKDLFVRNGIPAPE
                     VHLTAFFNLFIFMIIFNGFSVRTEKYNLFEHISQNTSFVWVMAMILGLQVIFTYIGGN
                     ILRTTALTVNEWIIIASMALVIIPVDMIRKYIVNSIERSRA"
     gene            9273..9536
                     /locus_tag="VGA21_14715"
     CDS             9273..9536
                     /locus_tag="VGA21_14715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651813.1"
                     /translation="MQTNKFCYRPDKIPALQQPENSRFFPENLRPMGLVVYQNTLGGK
                     HSKELHPFPITKLAGNCSFPNFGLFFYPLSQFIFAFTYPCLAN"
     gene            9736..10776
                     /locus_tag="VGA21_14720"
     CDS             9736..10776
                     /locus_tag="VGA21_14720"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013453657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX9651814.1"
                     /translation="MELGLNPEKISAAAQFATQNEYSGPKDLRVAILGSFAREPYMQI
                     VGPTRERGGPAGMIVKNGYVTATWGDLDRVDMTFSVTKSYLSTTVGLAFDEGRMALKD
                     PVAKYVWDGTFEGEHNSKITWHHLLNQSSDWSGSLFGVPDWSDRPPREGSIDDWKNRK
                     LNEPGTFFEYNDIRVNLLAYSILQVVRRPLPELLKERIMDPIGASSTWRWYGYETSWV
                     DIDGTKVQSVSGGGHFGGGMFINTYDQAKFGLLFLREGNWNGQQLISKKWIELLQQPS
                     EANESYGYMWWLNKGERKWEGVSDSVFYAAGFGGNYIIVDKEHDLLIVTRWLEPSKLG
                     EFAKMVLDLLEK"
BASE COUNT         3276 a         2117 c         2318 g         3248 t
ORIGIN      
        1 gaacttaacc tggatttaag cggcgtgaaa aattatgatg agttggttag ccacgttgct
       61 gatgcagttg caaaagctaa tcccggtgat tggatagttg gtcgtggctg gcatcaaagc
      121 aaatgggatc ctgtaccgga agaaatggta gatgggtttc aaactcataa gaaactcagc
      181 gagatttctc ctgaaaatcc agtatggtta tcgcacgcca gcgggcatgc cggttttgca
      241 aatgcaagag ctatggaaat tgcgggagta aatccactct ctatagaaag tttggaacag
      301 aatgttgaag ggggtgaaat tattactgat gcattgggga atcccactgg aattttcagt
      361 gaaaccgcac agggtctcat caggagacat attccggaaa atacacctgc aatggatcgc
      421 cgggctctgg aattggcgat aaaggcttgt caaagaaatg gtataacaag ttttcatgat
      481 gcaggtactg gacaaggtac cattgatctg atgcagcaat tcaaagagga aggaattctg
      541 ggggtgagga tgtatataat gcttgccggc aggagcgaag aactaattaa tcaatggctg
      601 gaaaagggac ctgtgcacga tactgacgat caactcttaa cgattcgggc aataaaactt
      661 aatgctgatg gtgccctggg aaatagaggc gcctggttac tggaggaata tacggacaga
      721 cagggacatt tcggatttgc taccatgccg cttgagtata tggaacgcgt aactcgtgat
      781 ggtctgagtg cgggatttca ggtgaacaca catgccatcg gggacagaac caatcgggaa
      841 atacttgaca tttatgaaaa agtattaact gaaattcctg agaaggcaac ggaccatcgg
      901 tttagaattg aacatgcaca acatcttagc ctggatgaca ttccccgatt tggcgaactg
      961 ggagtaattg cttctatgga agcaatccac atgagtagcg accgaccatg ggcaattgac
     1021 agacttgggg aaaagcgaat tgttgatggc gcttatgttt ggcagaagct gcttcaatca
     1081 ggcgcgataa ttgtgaatgg aacggatgtg cctgtagaac cgattgatcc aattgcatgt
     1141 ttttatgctt cagtaagcag gaaaaccctg gctggaatac cggaaggtgg atttgaacca
     1201 gagcagaaaa tgtcaaggga agaagcactt cgttcgtata cgcttgatgc agcttatggt
     1261 gaatttgctg aagaggttaa aggatcgatt tcacctggta agttcgcgga ttttacagtt
     1321 tttgatcagg atattatgac ggttcctgag aatcaaattc tggatactaa agtgactatg
     1381 actgtattca atggtaaagt tgtgtatgat ggaaatcctt caatgtagat tatttaatca
     1441 atgaaaatct tattcacttt tatggtatta ctaattgtag cctgcacagc tgagaaaaaa
     1501 gttggagaag atagttcaag accggaaaga gaaatttcaa gtcctcgaag agaagctacc
     1561 gggaatgcaa gcggaataaa catcatggtt gattatggaa gccctgcagt aaaagggcgt
     1621 agaatctggg gtggcctgga aaaatatgat cgcgtctggc gagccggtgc caatgaaacg
     1681 actgcaattt catttgataa agatgcatta attaatggtg agccgattga caaaggtaaa
     1741 tatgcaattt ttattattcc aaaagagaat gaagcctgga ttgttatttt caaccaggat
     1801 tgggatgaat ggggcgcatt cagatacaac caggaaaggg atgtccttag gtttactgtt
     1861 agcccggagt ggagtgataa cattcatgag cgactggaat attcaattga agaaggaaac
     1921 cttaccttcc gatgggaaaa agttagctta tcaatggctg taagttcaag aaactgattt
     1981 tattacaatc acggttagca tttggagtag tactaaataa ttaatctctg aaaatagatc
     2041 tttgagttgt ttttgcccca gctctttcat ttaccttagt caaataaatt tcaacaaaga
     2101 aaattattta aagatgtcaa agaaaaaagt aacaggaatc ggaggaatct tctttaagag
     2161 tgaggatcct aaaaaattga atgagtggta tgaaaagaat ctgggattgg ttttgaatga
     2221 gtatggctca ctttttgaat tccgaatgtc tgataattct gatacaaaag gttatttgca
     2281 gtggggtccc ttcacaaaag atacccgata ttttgatcct tctcaaaagc aatacatgat
     2341 taattacagg gttgaaaatc ttgaagaact ggtagaagaa ttgaaaagtg ccggcgtaga
     2401 ggtcgtagat gaaatagaga tttacgaata tggtaaattt gtgcacatta tggatcctga
     2461 agggaataag cttgaactat gggagccggt ggatagtgtt tttacgaaaa tgtatgaagg
     2521 taaaactacg aaatagctac ttttcttttc tgattttggc ttgtttgatg tgggccaggt
     2581 gatggttgct gtgccatgaa taaagtgccg taaccacaaa aagtttataa gtcttgttat
     2641 attcaggatg aataaattca aggttgaatt ggtcgtcatt caatgttttc aggaggtaaa
     2701 cccatcgctt gtgtaaccca tcaaggatgt ttaaggagtg agctacagga gtgtcttttg
     2761 catccggtaa ttcagcccac atgttttcaa gataaggctt tattgttgga ctcctttcag
     2821 ttaaagttaa tttgaatctc ataaaactat tcatgtggct atctgccaaa tggtgaataa
     2881 cctgcctgac tgtccaacct tccggccgat accggtagtc caactcttcc ttaccgagat
     2941 attcagtttc actttttatc cttgcgggta attgttcaat ttgattgatc cagtcatcaa
     3001 ttgaatcttt gctgaaagct tccggtgcat tgaactttcc tatagggtaa cgcaagtcgg
     3061 gtgtttccat gacacattaa tttattacaa attacgcaaa attttcagag cattaaactc
     3121 cgctgatgag cgcaaaaata gttaagaaat acaaatcagc cgaggaattt aattctggaa
     3181 ggaagacata ctatccgctt aggaacaggg gcaataagtt ctccatctga ttctgccgca
     3241 aatgtgactt cacctgaatg aatttcaaga tccctggcct caaaatagta tactctgggg
     3301 tgtttcaact tctttgattg aaagatgtaa ggtaattggg tcaaatattc ccacatactc
     3361 acctcaccaa caatagtaat ggagaacagc ccgctaaatg gggaagcatc aggtgcaaga
     3421 ctcatacctc cgcccatgta tttaccgtta gccaccacaa tcatgaatgc tttgccttcc
     3481 catttaaaat tatctccaac tatcttgaca gcagtaggac ggtaggaatt gaatgcctgc
     3541 ataatggatt tgaaataaga tttctttcca gtgccttttg agatttccat tttccggata
     3601 acctcggcac tgaagccgaa tgacacttca ttatgaaaat aaacatttgt tccatccgcc
     3661 agggtaagtt gcccgatgtc aatttttcta atggatggcg agtgaaggcg ccgggataaa
     3721 tctgcaattt tttttgacaa gttaaagttc cttgaaaaat cattgccggt accaatggga
     3781 aattgtccga tgatgataga atccttgtcc tgactgcctg aagtcatgta accattcagt
     3841 atttcgtgca gcgtgccatc accaccatac ccaataatta gcgtagttcc cgactcaatt
     3901 gctttcctgg tcaggaggat tgaatcaccc tttttttcag taaacaggta atcaatatct
     3961 ttatttgaag tcaggtacag ttggggaagg atgttcttcc tgaaagcaga ttgggaatga
     4021 taaagagagt tgacaacaaa agttattttc ataaattatc ccagagtggt ctgtaatagt
     4081 tggaatattt catttgaacg cagactaacc cagaaatttt gataatagca ggtgaaagtg
     4141 atgaactccg atttctcttt tgggagaaaa cctgccagcc ttgtatagca tagaatttac
     4201 gcccttctga acactttcta caacaaactc atcttcccgc tcaaccttgt ccaataaagc
     4261 gccggcactg tttgtcattt tagattcatc atgcataaat atttgaaaaa taactttaca
     4321 acggtctttt gttaaaggtt gaacaatatt gacagataaa ccccacggat aaaagtttaa
     4381 catcagattt ggaaatatcc aataataata tgccgataca ttctttccat agtcaatgtg
     4441 gccctcggga agatcaaatt tttcagttcc accttttgca tatccgattt gcaggtttcc
     4501 atattcaaaa gttttggttt cataagttcc ataatcgatc gcctcattta aatcattatg
     4561 aacaaatgga atatgaaaac cttccagata attgtcgcaa tacaaagccc aatgcgagtt
     4621 caccaggtat tctcttgata actccggttt aaatgtaaaa ttctccacag gcaggaaacc
     4681 ggttctttcg ttcagaattt tttcaatttt gataaattca taagcaggat ttaagctaag
     4741 aaaaaaaaat tgcttccatt tagccaatgg gatttttgga agattatcgc aattggaagg
     4801 aaagttttct gcttctttga attccggcat aaattcaaat ttaccatcca acgcaaattt
     4861 ccttccatga tatccacaaa tcagtttact tttcttagcc ggcgaattta tcaagacatt
     4921 tcccctgtgg gtacacacat tgctaagggc cgccacagaa tcattttcat cacaggttag
     4981 caaaagcggt tcatccatga atccgggata aaaatttatg gggtaaaggt tagtcttcag
     5041 attaaacaat ttttcatttc ccaccaggtg ccaggtaaca gcaaaaactt tttctttgaa
     5101 agaattaaaa acttcatcgc tcctgtagaa cgcggcgggt aacgtctcag cttttgatat
     5161 ttcctgatgg atagaaaaat gattcattat ttaaatgatt cattatttaa aacaattacc
     5221 aaaaatatga aaatatcggt attatcttag aaaatgcaaa ttcctgacgc aaaacccaaa
     5281 gtcggattct tgactgccac ctcactggtt gttggcaata tgataggttc cgggatattt
     5341 ttattgccgg caactctcgc gttttatggt ggaataagca tttttggttg ggtaataagc
     5401 gggattggtg ccatttttct cgcgctggtt ttcggaagat taagcaagct tgttcccaac
     5461 tcaggaggcc cgtatgcata tacgcgtgaa ggattcggcg atttcgccgg ctttatagtt
     5521 tcctggggat attggatttc aatcatttgt acaaacgcag ctatagcggt tactttggtt
     5581 agttatttat ctgttttcat cccggttata gccgcttcaa atctaacgtc agcacttatt
     5641 gctgtgggaa ctgtttggtt cctcacctgg atcaatagtc tgggcataaa gagaggtggt
     5701 aatatgcagg tgataactac agttctgaag ataattccat tgctggcagt atcaattgcg
     5761 ggtattttct atctgaacat cgaccatttt caaccctgga atataagcag tcaatcaaat
     5821 atcgatgctg ttggagccac cgttgcgtta acattttttg cattcatggg catggagtct
     5881 gcgactatac cttctgaaga tgtaaagaat gcatcaattg taattccaag ggcaacgata
     5941 accggtacta tgctggtgct gttaatttat atgctgagtt atgtggcttt aatggggcta
     6001 attccaccgg aaagtttaca gaagtccact tcaccttttg ctgatggagc cggtttgatt
     6061 tggggtgata ccggcagata tattatcggg ctgggagcaa tcatcagcac gttcggtgcc
     6121 ttgaacggct ggatattatt gcagggccag gtaccatacg cgatagcgag ggacagatta
     6181 tttccgcata gtttaagcaa ggtaaatatc cataaggcac cggttaccgg gctggtagta
     6241 tcaagtattt tcataactat tctgataatt tcaaacttta caaagggact tgtaggcatg
     6301 ttcactttca tgatattgct gacaactgtt gtagtagtct ttccttacct tttctgttca
     6361 cttgctgaaa ttttaattct tattaaaaga aaaaagagca agactaaatt cataaaacca
     6421 ttgctggttg gaattccatc attcctatat ctgatgtggg cgttaaccgg tgtgggaaag
     6481 gatgaagttt attacgggct cttattaatt attgcaggta tcccttttta cgccctgatt
     6541 aaatataaaa caaagcgtga gtaattctac gatttgagta tcatgccctg cttctttcga
     6601 tactatttac aatgtattta cgaatcatat caacgggtat tatcaccaaa gccatcgatg
     6661 caattattat ccattcgtta actgtcagtg cggtagttct aagaatattt cctccgatat
     6721 aagtgaaaat tacctgtagt cccaggatca ttgccattac ccacacaaag ctggtattct
     6781 gacttatatg ttcaaacagg ttatattttt cggttcttac gctgaagcca ttaaaaatga
     6841 tcatgaaaat aaagagatta aaaaaggctg ttagatgaac ctcaggagcc gggataccat
     6901 ttctcacaaa tagatctttg aaaggaggga aagtgaggaa gaatatagaa aagaaggtga
     6961 tgaaaagacc gctggttaaa atggccgaaa ccatattctt tgtaagaata ttttcatctc
     7021 tgttaacagg tggctcttcc atataccgca gcaaagcagg ctcaccccca aatgctattg
     7081 ctgcaagggt atccataata atattaatcc ataatatctg gataatagta agcgggaaat
     7141 ctactccgat aagagggcca aggaatgcgg tacaaacagc ggcaacattt accgtaagct
     7201 ggaaaacaat aaatttacga attgacttaa aaatggttct tccatatctt atggcattac
     7261 tgattgagtg gaagttatca tccaggatca cgatatctcc ggcttccttt gaaacttctg
     7321 atccactgcc cattgctaca cccacatcgg catgcttcaa tgcggcggaa tcatttacgc
     7381 catctccggt catccccaca actttaccaa gggatttgga aattctgacc agcctgctct
     7441 tatcagaagg aagtgcccgg gcaataattt taagattgcc aagtaccctc ttaatatcct
     7501 catcactatg ttcgcttaat tctgaagata ccattactac atcatccgac ctggtcagca
     7561 atccaacttc tgtggctact gatgaggcag ttcctttttt gtctcctgtt atcattacaa
     7621 cctgaatacc cgcattctgc acttctttaa ttgcacggaa agattcggct ctcacttcgt
     7681 caccaatacc tacgataccc acaaggcaag cactattggg aatagagtga tcatctttga
     7741 ttaattcatt ggttaccgca agggcaatca gtcgaatacc tgcttcggcc agctcatcca
     7801 tttctttata taatgcatcc gtacttgtta atggtacctt tgttccatca tcctcaaact
     7861 gatgtgtaca ctccggtatt aatacttcag gcgcaccttt gatcaaggtt atcctggttg
     7921 aatcaagccc tttgatttga taatcaatct ttacctgcgt ggctgaaaat tttcgttcac
     7981 tattaaatag aattttcttt tcgccttcag cgcccaactt catggttttt tcaatttcat
     8041 tttttgaaac gaaagcaata agcgctcttt cagaaccatt accaccaatt acgtcacctt
     8101 cgggagaaat atagcttgaa gaattttcga taactgataa tcccaacagc tccttgagtt
     8161 caagtggaat tgaattgaag tcagggaaaa tagttccggc actgttaata aataatttgg
     8221 gctcaagttg gcctttggta attgtacccg ttttatcaga aaacaaaata tttagggaac
     8281 ctgccgtttc aatacctaaa agctttcgca caagaacttt ttcgaccaat agtttccgca
     8341 tgttaagaga gagcacaatg gcgatcatca ttggcagccc ttccggaacc gcggcaacga
     8401 caataattat ggaaagaacc aacgcatcaa gaaggtctgg aatgagaatc tcccactggg
     8461 acaaataagc cattattttg tctccttcaa aaccattggc caccactatt ttattgaaag
     8521 tgaatgcgat agcaatgaaa atcgcggcta tatatccgaa ccggcttata agttgagcta
     8581 atcccttcaa ttttacctga aggggtgaaa gcctcccttc tgcttccgcc agttctttgg
     8641 caaggtgacc ataaaaggta ttattgccaa ctgtgtcaac tcttacaaca ccttctccat
     8701 catcaacaac tgaaccccgg aatatcaggt gcggatcagc aaggtctttg gcctttggta
     8761 ggtagtcaaa tggcattttg gtcttataaa cggagtcaga ttcaccggta agagaggcct
     8821 gattgactct taattctcct tttaccactt tgccatccgc gggtacttta tctccggcct
     8881 gaagcagaac ataatctccg gtgacaattt cacctaccgg tatttttcta agctggccgc
     8941 ctcgaaatgc agtattttca atgcgggaag cttcttcctg aagtttctgg aaagatgatt
     9001 catttttata ttccgagaat gtggaaacca atgtggccaa agcaacggca attgcaattg
     9061 ccagtgcttc ataccattca gttaattcaa aaatggataa aacaaaaatt atcaccaggg
     9121 cagcggtgag tataattatg atagggtctt gaaaattacc caccaacttg ctccagaaac
     9181 tcttaatttc atacggactg agttcgtttg agccgaactt cttcctggaa tctagaactt
     9241 cctgggtact aagaccctta aattcgtatt tcatgcaaac taataaattt tgttaccgcc
     9301 cagacaaaat tcctgcgttg cagcagcccg aaaatagtag attttttcct gaaaacttac
     9361 gtccaatggg tttagttgtt tatcaaaata ccttaggtgg gaaacattcg aaagaattgc
     9421 atcctttccc aatcacaaaa ttggcaggaa attgctcatt tcccaatttt ggcctatttt
     9481 tttatccttt atcgcagttt atttttgctt ttacttatcc atgcctggca aactaactgt
     9541 aattcagtga gtttatattg atatcgcctg gtttcgttta tttttgtagg acacaaatat
     9601 ccaaggcgat tcataatgag tataaaattc caattgaaat attacttact cattgtcctc
     9661 ttaatatcct cattaacagg gacatgtcaa aatagttatt atccaacccg ggctgaatgg
     9721 caatccaaaa caccaatgga gctgggtctg aatccggaaa aaatatcagc tgcggctcag
     9781 tttgccacac aaaatgaata ctccggtcca aaggatttgc gcgttgcaat attaggttct
     9841 tttgcccgcg aaccttacat gcagattgta gggccaacca gggagagagg cggcccggct
     9901 ggaatgattg taaagaacgg ctacgtgacc gcaacctggg gagatttgga tagggttgat
     9961 atgactttca gtgtaaccaa gagttacttg tctaccacag tgggacttgc ttttgatgaa
    10021 ggtcgaatgg ctttgaaaga cccggtcgcc aaatacgtct gggatggtac atttgaaggt
    10081 gaacataatt ccaaaatcac ctggcatcac ctgcttaatc aatcctcgga ttggtcaggt
    10141 tccttgtttg gagtacctga ctggtctgac cggcctccca gggaaggcag tattgatgac
    10201 tggaaaaacc ggaagctcaa tgagccggga accttttttg aatacaacga catccgggtt
    10261 aatttactgg cttattccat acttcaggtt gtaaggcgac ccttgcctga actgttaaaa
    10321 gaacggatta tggatcctat tggggcttca tcaacctggc gctggtatgg ttatgaaacc
    10381 tcgtgggttg atatcgatgg aaccaaagtt caatccgtga gcggtggtgg tcatttcgga
    10441 ggtgggatgt ttattaatac atatgaccag gccaaatttg gcttattatt tttacgcgaa
    10501 ggaaattgga atggtcaaca actgatttca aagaaatgga ttgagctgct ccaacaaccc
    10561 tctgaggcta atgaaagcta tggatacatg tggtggctaa ataaaggaga acgaaaatgg
    10621 gagggagttt cagattctgt tttttacgcg gcaggatttg gcggcaatta tattatagta
    10681 gacaaggagc atgacttgct gatagtaaca aggtggctgg aaccttcaaa gcttggtgaa
    10741 tttgcaaaaa tggttcttga tttacttgaa aagtaaaaat tgctagttcc ccgaaaaaat
    10801 atcatcaagg cttgaagcga taccaattct tacgccaaaa tctctgtatg atccattttg
    10861 gaaggatgaa acaaactcca acctgaatat tcggaggata taatcaatac tatatccaaa
    10921 ttctgtgtag tggtttgcag tgggagcttc cagatgatt
//
LOCUS       DASWHX010000230        13730 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_280058, whole genome shotgun sequence.
ACCESSION   DASWHX010000230 DASWHX010000000
VERSION     DASWHX010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 13730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13730
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280058"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(102..500)
                     /locus_tag="VGA21_14725"
     CDS             complement(102..500)
                     /locus_tag="VGA21_14725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651815.1"
                     /translation="MEANKYLNLCQVNQEFLSKLTDKFPDLYFDWKITVLFYCSVHII
                     RGLAESNGDSLGNRHNDVFDYLQKVNSGDKIYKAFRTLYRNSRDVRYSGFTTRENFEF
                     FCRIKFEESKRMYGQLKSWIETKGVKVEVA"
     gene            complement(478..1005)
                     /locus_tag="VGA21_14730"
     CDS             complement(478..1005)
                     /locus_tag="VGA21_14730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651816.1"
                     /translation="MRNTTSDTQIEEGRKLTITGMLMALEGFLKELSGQASDALSLYD
                     IGEKNELILQLEKSIDDPLKTMSDINESFKAGFLGFKEQLITKFVASKSKLVERGFMV
                     NDDGATQVFISLNTDTDESREVFYDLLDKYEKKKIFDDYPIIFHFINTHDLDDAVSGI
                     EGIKKIPLDGSKQIS"
     gene            1161..1334
                     /locus_tag="VGA21_14735"
                     /pseudo
     CDS             1161..1334
                     /locus_tag="VGA21_14735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_040609789.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            1334..1804
                     /locus_tag="VGA21_14740"
     CDS             1334..1804
                     /locus_tag="VGA21_14740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651817.1"
                     /translation="MEFEVYELHEQILLYLYEYRNTGNLYNLLDKFGNLNRNLLREKV
                     DELYSKGFIHVIPEYYVQVVSLKPKKNRGVTVQTGNRQNKPERILARILTTGVEYVKK
                     EILNQNRQSSNINIGKGSNVNFGTTKSQSVKSSNSQRLISAKKEMSIIKRCSLE"
     gene            1955..2027
                     /locus_tag="VGA21_14745"
     tRNA            1955..2027
                     /locus_tag="VGA21_14745"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1988..1990,aa:Gln,seq:ttg)
     gene            2077..3015
                     /locus_tag="VGA21_14750"
     CDS             2077..3015
                     /locus_tag="VGA21_14750"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009579085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HEX9651818.1"
                     /translation="MQSVKIFGGNGTRKLAEEIATSFGKPLGSVSIRKFSDGEIWVSF
                     DESVRGCDVFVVQSTGAPSDNLVELILMIDAAKRASADYVTVVVPYFGYARQDRKDQP
                     RVSIAAKLMANILSAAGADRLMTVDLHADQIQGFFDIPVDHLDGSSVFVPYLSNLKSD
                     ILVFASPDVGGVKRARNYAKYFGADMVVVDKHREKANQVASMQLIGNVEGADVILVDD
                     LIDTGGTMCKAAKLILEKGAKSVRAVVTHPVLSGNAYKNIDESVLEELVVTDTIQVKG
                     SSKKIRVLGIADLFAKAIRKIHDHESTSSLFIKGFN"
     gene            3038..3724
                     /locus_tag="VGA21_14755"
     CDS             3038..3724
                     /locus_tag="VGA21_14755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002706110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HEX9651819.1"
                     /translation="MKTVEIIGYKRANLGKSEAKRLREEGNVPCVLYGDQKQVHFYSP
                     MILFNELLFTNEAHFVHLNVEGEEYSAILQEVQFHPVSDIILHADFLALHEDRRIKMS
                     VPVRLKGTSPGVAKGGTLIQKRRSLMIKAFPKDMPDHIDVDISELDFHHAVKVNKVVE
                     ENFEIQDTKIASIAVVEVPRALKTVEEEAAEAAAKLAIEGVEGGEVAEGEEGEKKAES
                     KEGKEGKDNK"
     gene            3831..4388
                     /gene="pth"
                     /locus_tag="VGA21_14760"
     CDS             3831..4388
                     /gene="pth"
                     /locus_tag="VGA21_14760"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008630750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEX9651820.1"
                     /translation="MKYLIAGLGNIGAKYELTRHNVGFLILDRLAERLGGNFTPDRLA
                     EISRVKFKGKQLVLVKPATYMNQSGKALNYWLQQEKINKQNLMVIADDIALPFGKIRI
                     RAKGSDAGHNGLKNIEELLMGPDYPRLRFGIGDDFAQGQKVDFVLSNFNQQEFEALPV
                     LMDNACEAILSFISIGLERTMNIFN"
     gene            complement(4381..6429)
                     /gene="metG"
                     /locus_tag="VGA21_14765"
     CDS             complement(4381..6429)
                     /gene="metG"
                     /locus_tag="VGA21_14765"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007657514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEX9651821.1"
                     /translation="MSKEFKRYTVTAALPYANGPIHIGHLAGVYIPADIYVRYLRSKE
                     KDVVFVCGSDEHGVAITLKARKEGLTPQQVVDKYHSMIKESFENFGIGFSIYHRTSSS
                     THHETASSIFLKLYEQGKLEEKTSEQFYDRQEKQFLADRYIIGTCPNCGNTNAYGDQC
                     ESCGTSLSPAELINPRSAISGAKPVLKETRHFYLPLNEYESWLRGWIIEGHKNDWKIN
                     VYGQCKSWIDNGLKPRAVTRDLDWGVPVPLKGMEGKVLYVWFDAPVGYISATIDWAKE
                     NGIDWKPYWQDKDTKLLHFIGKDNIVFHCIIFPVILKSHGDYILPENVPANEFLNLEG
                     NKISTSRNWAVWLHEYLEEFPGKEDVLRYTLCANAPETKDNDFTWKDFQARNNNELVA
                     IFGNFINRAAVLTDKFFNGVIPERGEIFSIDEKLIAELNAYPDKIASALEEYKFREAL
                     SLFMDLARLGNKYLAETEPWQLIKTNPERVKTILNLALQVAANLQILGHPFLPFTSKK
                     LGSILKLESYNWKDSGSIDLIKPGSKLGKAELLFEKIEDDTIQYQIQKLENTRKENMQ
                     SNQLPGLRPEIQYDDFAKLDIRVGTILEAEVVPKSKKLLKIKLDTGLDTRTVLSGISE
                     QYSPEEIIGRQVCVLANLAPRKMLGLESQGMILMSEDSDGKLNFIAPSSQTGNGSQIS
                     "
     gene            6571..8379
                     /gene="typA"
                     /locus_tag="VGA21_14770"
     CDS             6571..8379
                     /gene="typA"
                     /locus_tag="VGA21_14770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010608086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEX9651822.1"
                     /translation="MTSIRNIAIIAHVDHGKTTLVDKMIYTANIFRENQEKKELILDS
                     NELERERGITILSKNVSIVYKDTKINIIDTPGHSDFGGEVERVLKMADGVLLLVDAFE
                     GPMPQTRFVLNKALGLGLKPIVVVNKVDKPNCRPDEVHELVFDLMFNLGATEEQLDFV
                     TLFGSSKQGWMSTDWKEPTNNITPLLDAILKVIPAPVVKEGTPVFQITSLDYSSFIGR
                     IAIGRIYKGILKEGDRLGLTKRNGKTVPVKLKELHVFEGLGKKKVNDVACGDICAIVG
                     IEDFEIGDTISSLENPEPMPRIAVDEPTMSMLFTINNSPFYGKEGKYVTSRHLRDRLF
                     RETEKNLALRIEETSSEDKFIVFGRGILHLSILIETMRREGYELQVGQPQVLYKNIDG
                     LRCEPVETLVIEVPREYSGKAIEMVTQRKGELLIMEPKQDVQHLEFSIPARGIIGLRN
                     SLLTATAGEATITHRFKSYEPFKGEIAGRTNGSLISMEAGPGTAYSIDKLQDRGRFFV
                     DPGENVYTGQVIGEHSRQSDLVVNIQKGKKLTNIRASGTDDAARIVPKIKFSLEEALE
                     YIQKDEYVEVTPKSIRMRKIYLDENERKRKANVEAV"
     gene            complement(8393..9361)
                     /locus_tag="VGA21_14775"
     CDS             complement(8393..9361)
                     /locus_tag="VGA21_14775"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEX9651823.1"
                     /translation="MKTKGIDNAKLGLFVLAGLVFLVFSLYMIGSNRNIFGSTFIISA
                     KFYNVNGLMAGNNVRFSGIDVGTVKKLELDSDTSVNVVMVIDEKVRRFIKVNAIASVG
                     TDGLMGNKLININAQLGNAAQIKAGDVLATMKPVETDEMLRTLSTTNENIAIITEDLK
                     KITLKINSSNSLWSLLSDTTIAKDIKDAVLNIKTASKNASNAVNEIGDLAYAIGEGDG
                     LLGTLISDTTLSYNLSHSLGKVDSAASQLSILMTDLQQMALQIKDGKGTAGAILYDSL
                     MTVKLNTSLDNIEQGTARFNENMEALKHNFLFRRYFKKLEKQSKEN"
     gene            complement(9366..10103)
                     /locus_tag="VGA21_14780"
     CDS             complement(9366..10103)
                     /locus_tag="VGA21_14780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009581237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX9651824.1"
                     /translation="MNSGVIEIEKLNKSFGENHVLSDFTVNLNKGENLIVLGKSGSGK
                     SVLIKCIIGLLTPDSGSIKVFETEIAELSHEELDQIRAKVGFLFQSNALYDSMTVREN
                     LEFPLRRHWTKAKRKTEEVEKMVLEALGNVGLAHTVDMMPAELSGGMRKRIALARTLI
                     LKPEIILYDEPTTGLDPITAKEISELILEIQAKYNTSSMIISHDMNCIKITSNRVILL
                     IGGKCYADGTYDDLKNSSDSSVKQFFE"
     gene            complement(10105..10884)
                     /locus_tag="VGA21_14785"
     CDS             complement(10105..10884)
                     /locus_tag="VGA21_14785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009577870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9651825.1"
                     /translation="MRYSKFFRSTRLFLAEVGGISKFLARFFSEGFRPRYEFQEFIRQ
                     CFWVGYKSLPLVATTAFIMGLVLTIQSRPTLVEFGAESMLPAMVSVSLIREISPVITA
                     LICAGKIGSGMGAELGSMKVTEQIDAMEVSGTNPFKYLIVTRIAAATLMLPVLSSLSN
                     AVSLYGGYLGVNIRGSVSWHLYWNQVFHALSYGDFVPSLIKTFFFGFAIGLIGCYKGY
                     NTQKGTEGVGRSANSAVVVASLLVFIIDLVAVQITDLLGLT"
     gene            complement(10984..11853)
                     /locus_tag="VGA21_14790"
     CDS             complement(10984..11853)
                     /locus_tag="VGA21_14790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018477897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9651826.1"
                     /translation="MDNGMLKEGALKDKVILVTGGGTGLGKEMATYMSRLGAKIAILS
                     RKEEVLKKSAQEMSSASGNEVMPITCDIRKYEDVEKSLEAIESQLGKLTSVLNNAAGN
                     FVSPTERLSHRAFDTVVDIVLRGSYNVTLAMGKKWLSENQKGTFLNIVTTYAWTGSGY
                     VVPSACAKAGVLALTRSLAVEWGKYGIRSNAIAPGPFPTQGAWTRLFPGDLAKKIDPI
                     QRIPLGRVGEYHELANLAAYLISDYSGYINGEVVTIDGGEWLKGAGEFNQFEMIPDAL
                     WDAYEQMRKGSKG"
     gene            11944..12894
                     /locus_tag="VGA21_14795"
     CDS             11944..12894
                     /locus_tag="VGA21_14795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013452350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX9651827.1"
                     /translation="MNHRIISLAALVLIFVSSCIAPKKYNEQLAENVRMQGEITRLTS
                     DLSVAKKRITDLNSKLDSLSQDNNMTKDNLAATSSSLENLQNEHEKLQTLYDNVLKNS
                     GKLNRDLAEQQERLLTMESDLESAKAKNDQLSIDLAEREKKVSELEKILADKEAAVLA
                     LKKKVTDALLNFGEKELTVEVRNGKVYVSLAEQLLFKSGSTSVDEKGRIALQQLAAAL
                     KDSKDIHIMVEGHTDNVPISRASQYMNDNWDLSVMRATTIVRILTNYGVSPEQVTASG
                     KGEFSPVMENTTPENKQKNRRTEIILTPNLDELFKILEAN"
     gene            12953..13366
                     /locus_tag="VGA21_14800"
     CDS             12953..13366
                     /locus_tag="VGA21_14800"
                     /inference="COORDINATES: protein motif:HMM:NF020360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HI0074 family nucleotidyltransferase
                     substrate-binding subunit"
                     /protein_id="HEX9651828.1"
                     /translation="MNKPDIRWKQRFHNFNEAYQRLKEAVEIPELNELERNGLVQRFE
                     FTLEMGWKTLKDFLQHKEFSFRPSPKDTLRLAQQSGYIDQAQELIDGLELRNMLSHDY
                     SGAKFLESEEKFRNIIYPALEKLYLFLKSEFEKSE"
     gene            13363..13680
                     /locus_tag="VGA21_14805"
     CDS             13363..13680
                     /locus_tag="VGA21_14805"
                     /inference="COORDINATES: protein motif:HMM:NF037100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEX9651829.1"
                     /translation="MKNGLSKRDYETIKGILEKYPEITLVNLFGSRATGTFHAGSDID
                     LAIMNKGVTDEIIQRILSDFEESTLPYFVDIINYYTIEHKPFREHIDRVGQLFYQKKM
                     VNK"
BASE COUNT         4250 a         2702 c         2723 g         4055 t
ORIGIN      
        1 gtaaaggcgg tctgaaaggt ggcaaggcaa aggctaaagc gttaagtcct gaaaggagaa
       61 aagatagtta aaaaggcagc aaataagcgg tggaaaagag attaggctac ttcaacttta
      121 actccctttg tttctatcca agattttaat tgcccataca tacgtttact ttcttcaaat
      181 ttaatgcgac aaaagaattc gaagttttcg cgggttgtaa atccactgta cctgacatct
      241 ctggaatttc tgtataaagt ccgaaatgct ttgtaaatct tatcgcctga gttgactttt
      301 tgaaggtaat caaaaacatc attgtgtcta tttcccaggc tatctccatt tgattcagcc
      361 aatccgcgga taatatgaac actacaataa aacaatacgg tgattttcca atcaaaataa
      421 aggtccggga atttatcagt taatttggat aggaattcct ggttaacttg gcatagatta
      481 agatatttgt ttgcttccat ctaagggaat tttttttatt ccctcaattc cgcttacagc
      541 atcatctaaa tcatgagtat ttatgaagtg gaaaataata gggtaatcat cgaaaatctt
      601 tttcttttca tatttatcca gtaaatcata aaatacctca cgagattcat cggtgtctgt
      661 attcaatgaa ataaatactt gagttgctcc atcatcattt accataaaac ccctttcaac
      721 caatttagac ttggaagcga caaactttgt tattagctgt tctttgaatc ccaagaatcc
      781 agctttgaat gattcattta tatcagacat tgtttttagt ggatcgtcta tggacttttc
      841 aagctgaagt atgagttcgt tcttttctcc aatatcatat agcgaaagcg catcacttgc
      901 ctgtccggat aattccttca gaaaaccctc taaagccatt aacataccag tgattgttag
      961 ctttcttcct tcttcaattt gagtatctga ggtcgtatta cgcattaatg gagttgttat
     1021 agtgaagaga gcgcaaaatt agtacctaaa gatgaataaa cttagataaa gttgattaat
     1081 aattcaagta tatatacgtg gaaatagtaa aagtaactga aggaaatagt aataattatt
     1141 gagataaaaa tgaactctgc cttacaaatg cattttctaa gaaattagag aatcattgct
     1201 tgacaatagc cctgcacttt gtatattata acttcgttcg cattcataaa agtttgaggg
     1261 taactcctgc aatggaagct ggattggcaa aacggccaat gactatcggg gatttagtaa
     1321 acttaattga ttaatggagt ttgaagttta cgagcttcac gaacaaatcc ttttgtattt
     1381 atatgaatac aggaatactg gaaatcttta taatctgctg gacaaattcg gcaacttaaa
     1441 tagaaatctg ttaagggaaa aagtagatga actatattct aaagggttta tccatgtaat
     1501 tcctgaatat tatgttcaag tagttagcct taaacctaaa aagaatcggg gtgtaaccgt
     1561 acaaacaggc aataggcaaa ataagcccga acgaatactt gcaagaattt taacaacggg
     1621 tgttgaatat gttaagaaag aaattttgaa tcaaaaccgt caatctagta atataaatat
     1681 tggaaaaggc tccaatgtta attttgggac aacaaagagt caatcagtta aatcatccaa
     1741 tagccaacga ttaatctccg ctaaaaaaga aatgagtata ataaaaaggt gctcattgga
     1801 atagggattg cagtaatagc aggattaata ctttattacc tgtttggaat aggtggaggc
     1861 ggataatttc aaactgaggc taccaaatta tttcatgcct tttctattta aggaaataaa
     1921 ttatactttt gcagtccgaa ataagggcaa gaattgtccc atggtgtaac tggcaacacg
     1981 tctgattttg gttcagaaga gtctaggttc gagccctagt gggacaacta acccgatata
     2041 caccgggatt taatttagta tagcaatttc aatctgatgc agtctgtaaa gatctttggc
     2101 ggaaatggta ccaggaaatt agctgaggaa attgccactt catttggcaa acctctcggc
     2161 tctgtttcca ttcgcaaatt ctctgatggc gaaatctggg taagcttcga tgagtctgtc
     2221 aggggttgcg atgtttttgt tgttcagagt acaggcgctc cttccgataa tctggttgaa
     2281 ctgattttaa tgattgatgc cgctaagaga gccagtgcgg attatgtgac agttgtagtt
     2341 ccctactttg gctatgcccg ccaggacagg aaagatcaac cgcgggtttc catcgctgct
     2401 aaacttatgg ccaacatttt atccgcagca ggcgctgacc ggttaatgac agtggatctg
     2461 cacgccgatc agattcaggg attttttgat attcccgtgg atcatcttga tggttcttct
     2521 gtttttgttc cctatttaag caacctgaag agtgatattt tggttttcgc ttctcctgat
     2581 gttggaggag taaagcgagc cagaaattat gcaaagtatt ttggagctga tatggtggta
     2641 gtggataaac acagggaaaa ggcaaaccag gtggcatcca tgcagctaat tggtaatgta
     2701 gagggtgccg acgtaattct agttgatgac ctcattgata ccggcggaac gatgtgtaag
     2761 gcagctaaat taatattgga aaaaggagcg aaatcggtta gagctgtcgt aacacaccct
     2821 gtgttgtcag gaaacgcgta caagaatatt gatgaatccg tattagagga attagtggta
     2881 acagatacaa ttcaagtaaa aggatcaagt aaaaaaatta gagtactggg cattgcagat
     2941 ttatttgcaa aagcaatccg gaaaattcat gaccatgaat ccaccagttc attattcata
     3001 aaaggattta actagcaaca actaaataat aaattatatg aaaactgtag agattatagg
     3061 gtataaaaga gcaaatctcg gaaagagcga agcaaagcgc ttaagagaag agggtaacgt
     3121 tccatgtgta ttatatggcg accaaaaaca ggtgcatttc tattcaccaa tgattctctt
     3181 taacgaactg ctttttacta atgaagctca cttcgttcac ctcaatgtag agggtgaaga
     3241 atatagcgca attttacagg aagtccagtt tcatccggtc agcgacatta tactgcatgc
     3301 agatttcctg gcacttcatg aagacaggag aataaaaatg agtgtaccgg tcaggttgaa
     3361 aggcactagt ccgggagtag caaagggtgg aaccctaatt caaaaaagac gttccctgat
     3421 gatcaaagca tttccaaaag atatgcccga tcatattgat gtggatatat ccgaacttga
     3481 ttttcaccat gcggtaaaag taaacaaagt ggtggaggag aatttcgaaa ttcaggatac
     3541 caagatcgca tctatagctg tggtagaagt accacgagct cttaaaacgg ttgaggaaga
     3601 ggctgccgaa gctgcagcga aacttgccat cgagggtgtt gaaggtggag aggttgcgga
     3661 gggtgaagaa ggagaaaaga aagcggagtc taaagaaggc aaggaaggga aggacaacaa
     3721 gtaatttcga aacgacacac ttcaaaatcc cgcctaattg acgggatttt ctttttgcaa
     3781 ccaattgtca tcaaataaat gcaccctaag tttttcttaa ttttataccc gtgaaatatc
     3841 tcattgcagg attgggaaat ataggtgcta aatatgagct tacccgtcat aatgttggct
     3901 tccttattct tgaccggtta gcagaaagat tgggtggcaa ttttactcca gacagacttg
     3961 ccgaaatttc cagagtaaaa tttaagggca agcaattggt tttggtaaaa cctgccacct
     4021 acatgaatca aagtggtaaa gctcttaatt actggctgca gcaggagaaa attaataagc
     4081 aaaatctaat ggtaatcgct gatgatattg ctctcccatt tggcaaaatc cgtatcaggg
     4141 caaaaggatc tgatgccggc cacaatggat taaaaaacat tgaagaactg ctaatgggtc
     4201 ctgattatcc gcggctaaga tttggtattg gtgacgattt tgcacagggg caaaaagtag
     4261 attttgttct gagcaatttt aatcaacaag agtttgaagc attgcccgtt ttaatggaca
     4321 acgcatgtga ggccattctt tcattcattt caattgggct ggaaagaacc atgaacatct
     4381 ttaactgatt tggcttccgt tgcctgtttg tgaagatgga gctataaaat tgagttttcc
     4441 gtcagaatct tccgacatca ggatcatccc ctgagattcc agccccagca tttttcttgg
     4501 agccaggttg gccaggacac atacctgcct gccaataatt tcttcaggac tatactgttc
     4561 acttatgccg ctcagaacgg ttcttgtgtc aagcccggta tcaagtttaa tcttaagaag
     4621 cttttttgat tttggaacta cttctgcttc aaggatagtt ccaaccctta tatcaagctt
     4681 cgcaaaatca tcgtattgaa tttccggccg aagcccgggc agttggtttg attgcatatt
     4741 ctcttttctt gtgttttcta atttttgaat ttgatactga atagtatcat cttctatctt
     4801 ttcaaataat aattcagcct tccctaattt ggaacctggt ttaatcagat caattgaacc
     4861 actatctttc cagttataac tttcaagttt aagaatactc ccaagttttt tacttgtaaa
     4921 gggaagaaat ggatgtccga gtatctgaag atttgccgct acctgaaggg caagattcaa
     4981 aatcgtcttt actctttccg gattggtttt gatgagttgc catggctccg tttccgcaag
     5041 atatttattc cccaacctcg ccaaatccat gaacaagctc agagcttcgc ggaatttata
     5101 ttcttccagg gcagaagcga ttttatcagg gtaagcattt agttccgcaa tcagtttttc
     5161 atcaatcgaa aaaatttcac ctctttcagg aattactcca ttaaagaatt tatcagtaag
     5221 gacagcggcc cggtttatga aatttccaaa aatagcaacc agttcattgt tatttctggc
     5281 ctgaaaatcc ttccaggtga aatcgttatc ttttgtttcc ggtgcattgg cgcaaagagt
     5341 gtagcgcaat acatcttcct taccgggaaa ttcctcaaga tattcatgca accaaacagc
     5401 ccagtttctt gaagtggaaa ttttgttacc ttccaggttt agaaattcat tggcaggtac
     5461 attttcaggt aaaatgtaat caccgtgaga cttaagaatc accggaaata ttatacaatg
     5521 aaatacgata ttatctttcc caataaaatg aaggagtttg gtatccttat cctgccaata
     5581 cggtttccag tcgataccat tttcctttgc ccaatcaata gtagcagaga tataaccaac
     5641 aggagcatcg aaccatacat ataggacttt gccttccatc cctttcaatg gtacaggtac
     5701 gccccaatcc aaatccctgg taactgcacg aggcttaagt ccgttatcta tccaggattt
     5761 gcattgtcca tatacattaa ttttccaatc gtttttgtgt ccttcaatga tccacccacg
     5821 cagccagctt tcatattcat tcaaaggcaa ataaaaatgc ctggtctcct taagaacagg
     5881 tttagcaccg ctaatggccg agcgagggtt tatcagttct gccggactca gcgaagttcc
     5941 acaagattca cattgatcac cataagcatt cgtgtttccg caatttgggc aggttccaat
     6001 aatatagcga tcggcaagaa attgcttttc ctgccggtca taaaattgtt cgcttgtttt
     6061 ctcctcaagt tttccttgct cataaagctt tagaaaaata gaggaggctg tttcatgatg
     6121 agtggatgat gatgttctgt gatagatgct gaagccaatg ccaaaatttt caaatgattc
     6181 cttaatcatg gagtgatact tatcaacaac ttgctgcggt gttagccctt cttttcttgc
     6241 tttaagtgtt atggctacac catgctcatc agatccgcat acgaatacaa catccttttc
     6301 tttacttcgc aagtaccgga cataaatatc cgccgggatg taaactcctg ccaaatgacc
     6361 aatgtgtatc ggaccattgg cataaggtaa tgcagcagtg acggtatatc tcttaaattc
     6421 ctttgacatt tttttcaatc aaaaactggg caaagataaa gatttaaagg ggttttaccg
     6481 gattgaccac tcattttcaa agattaattt gagtggttac attttagact gaaacccttt
     6541 ttgtactttt gcacctgttt tcaataaaga atgacttcaa ttagaaatat tgcaataatt
     6601 gctcacgtag atcacggcaa gacaacactt gtggataaaa tgatctatac cgcaaatatt
     6661 ttcagggaaa accaggagaa aaaggaattg attctggaca gtaatgaact ggaaagggaa
     6721 cggggcatta ccatacttag caaaaacgtt tctattgttt acaaagacac caaaattaac
     6781 attatcgata cgccaggtca ctccgatttt ggcggtgaag tggaaagagt acttaaaatg
     6841 gcagatggcg tgttgctgtt ggttgatgct tttgaaggcc caatgcctca aacccgtttt
     6901 gtcctgaata aagcccttgg tttgggacta aagcctattg ttgttgtgaa taaagtagat
     6961 aagccgaatt gtcgccctga tgaagttcat gaattggttt ttgatttaat gttcaatctg
     7021 ggcgcgaccg aagaacagct tgactttgtt acacttttcg gttcatccaa acaaggatgg
     7081 atgagtaccg attggaaaga accgacaaat aatatcacac ctttgctgga tgcgatcctt
     7141 aaggtaatcc ccgccccggt tgtaaaagaa ggcacgccgg tatttcagat tacttcgctg
     7201 gactattctt cctttattgg aaggattgcc attggacgga tttataaagg aattctgaaa
     7261 gaaggcgaca ggcttggcct cacaaagaga aatggaaaaa ctgttcctgt caagcttaaa
     7321 gagttacatg tgtttgaggg gttaggtaag aaaaaagtta atgatgtggc ctgcggagat
     7381 atttgtgcta ttgtagggat tgaagatttt gaaattggcg atacaatctc cagcctggag
     7441 aatcctgaac ctatgcctcg tattgcagtt gatgagccaa cgatgagcat gttattcacg
     7501 atcaataatt ccccttttta tggtaaagaa ggcaaatacg taacttcacg gcatttaagg
     7561 gatcgtcttt tccgggagac agaaaaaaat cttgccctcc ggattgagga aaccagttcc
     7621 gaggacaaat tcatcgtttt tggtagggga attcttcacc tttccattct tatcgaaacc
     7681 atgagacgtg aaggatatga gttgcaggta ggacaacctc aggttcttta taagaatata
     7741 gatggactaa gatgtgaacc cgtagaaact ctggtgattg aggttccccg ggaatattcc
     7801 ggtaaagcca ttgaaatggt gacgcagaga aaaggagaat tgctaattat ggagccaaag
     7861 caggatgtgc agcaccttga attttccatt cctgctcgcg gaattatcgg gctcagaaat
     7921 agtttgctca cagcaaccgc cggtgaagca accataactc atcgctttaa atcttatgaa
     7981 ccatttaaag gtgaaattgc gggaagaacc aacggctcat taatttcgat ggaagccggc
     8041 ccgggaaccg cgtattctat tgataaactt caggatcgcg ggcgattttt tgttgatccg
     8101 ggtgagaatg tctacaccgg ccaggtaata ggcgaacaca gcagacaaag cgatttagta
     8161 gttaatatcc aaaaaggaaa gaaactgaca aacatccggg cttcgggcac ggatgatgcc
     8221 gcacggattg ttccaaagat aaaattctcc ctggaagaag ccctggaata tatccagaaa
     8281 gacgaatacg tagaagtaac acccaaatcg atccggatga gaaaaattta tctggatgag
     8341 aatgaaagaa aacggaaagc aaatgttgaa gccgtctgag acttttaggt ggttaattct
     8401 ctttactttg tttctctaac tttttaaaat accttctaaa caggaagttg tgtttcaacg
     8461 cttccatatt ctcattaaat cttgcggtgc cctgttcaat attatccaga ctggtattga
     8521 gtttcactgt cattagcgaa tcataaagta tagcacctgc tgttcctttc ccatctttta
     8581 tctggagagc catctgctgc aaatcggtca taagtattga taattgtgaa gctgccgaat
     8641 ctaccttccc cagggaatgg ctcaaattat aggaaagcgt agtatcagaa atcagggtac
     8701 ccagtaagcc atctccctct cctatcgcat acgcaagatc cccaatctca tttaccgcat
     8761 ttgacgcatt cttgctggct gtctttatat tcaaaaccgc atctttaata tcctttgcaa
     8821 ttgttgtatc ggataacaga ctccataaac tattgctgct attgatcttt aaagttatct
     8881 ttttcaaatc ttcggtaata atggcaatgt tttcattagt tgtactaaga gttctaagca
     8941 tttcatccgt ttctaccggt ttcattgtag ccaaaacatc ccctgctttt atttgcgcag
     9001 catttcccag ctgcgcgttg atatttataa gtttatttcc cattaatcca tctgttccaa
     9061 ctgatgcaat tgcatttacc ttgataaacc tccgaacttt ttcatcgata accataacta
     9121 cattcacgga ggtgtcacta tccagttcca atttctttac agtgcccaca tcaatgccgg
     9181 aaaatcttac attatttccc gccatcaaac cattcacgtt atagaatttt gcacttataa
     9241 taaatgtgga gccaaatata ttcctgttgc ttccaatcat ataaagtgag aagacaagaa
     9301 aaacaagtcc ggcaaggaca aacagaccaa gttttgcgtt atcaattcct tttgttttca
     9361 ttactttatt caaaaaattg tttaacactg gagtccgatg aattttttaa gtcatcatag
     9421 gtaccgtcag cataacattt tcctccaata agtaaaatta ctctgttaga agtaatcttt
     9481 atacaattca tatcatggga tattatcatg gatgacgtgt tatactttgc ctgaatctcc
     9541 aaaataagtt cactgatttc ctttgctgta attggatcca gtccggtagt tggttcatca
     9601 tataagataa tttccggttt aaggatgagt gtacgtgcca gggctattcg tttccgcatt
     9661 ccaccggata actccgcggg catcatatcc actgtgtgag caagacctac atttcccaag
     9721 gcttccaaaa ccattttctc cacttcttcg gtttttcgct ttgctttagt ccaatgtctc
     9781 cggaggggaa attctaaatt ctctcttact gtcattgaat catataaagc attactttga
     9841 aacaggaacc ccacttttgc ccgtatctgg tcaagttctt catgactaag ttcagctatt
     9901 tctgtttcaa acactttaat tgaaccacta tcaggagtca gcaagccgat aatgcatttt
     9961 attaaaaccg atttgccgga cccggatttt cccagtacaa tcaggttttc ccccttattc
    10021 aggttcacgg taaaatcact gaggacgtga ttctctccaa atgatttatt tagtttctct
    10081 atttctatta ctccgctatt cattttatgt caatcctaac aagtcggtaa tttgcacggc
    10141 aactaaatca attataaaca cgagtaatga tgcaactaca actgcagaat tggccgatct
    10201 cccaacgcct tctgtacctt tttgagtatt atatccctta taacaaccta tcaagccaat
    10261 ggcaaaaccg aaaaagaaag tctttataag cgagggaacg aaatctccat agctaagtgc
    10321 atgaaatacc tggttccagt acaaatgcca acttactgac ccgcgtatat ttacccccaa
    10381 atatcctcca tataaagaaa ccgcatttga caaacttgac aatactggaa gcatcaaagt
    10441 tgctgcagcg attcgggtga ctattaaata tttaaatgga tttgttcctg acacttccat
    10501 cgcatcaatt tgttccgtta ctttcatcga acccagctct gcacccatcc ccgaccctat
    10561 tttccctgcg caaatcaatg cagttataac cggcgaaatt tctctaataa gagaaactga
    10621 aaccatggcg ggtaacatgg actccgcgcc aaattcaacc agggttggcc tggattgaat
    10681 ggttaagacc aatcccatga tgaaagctgt agtagcaact aaaggaaggg atttataccc
    10741 aacccaaaag cattgcctga tgaactcctg aaattcgtat cgaggtctaa acccttcaga
    10801 aaaaaacctg gccaggaatt tactaatccc gcctacttca gcaaggaaaa gcctggtcga
    10861 cctaaaaaat ttggaatatc tcatttattt gaaagtccct tacctaaaga tgaagctcag
    10921 tgcaaaatat tcatttgact attatcattt gagcaaatga tgtttatcat cttggtaaac
    10981 aacctaccct ttactgcctt ttctcatctg ttcatatgca tcccaaagtg catccggaat
    11041 catctcaaac tgattaaatt cacctgcacc ttttaaccat tccccaccat caatggttac
    11101 tacttcacca tttatgtagc ctgagtaatc agagattagg taggcagcca ggtttgccag
    11161 ttcatgatat tcacccactc tgcccagtgg aattctttgg ataggatcaa ttttttttgc
    11221 taaatcccct ggaaacagtc ttgtccaggc accctgggtt gggaatgggc ccggcgcaat
    11281 ggcattagag cgaataccgt actttcccca ttctaccgcg agggatctgg taagcgcaag
    11341 tacgcctgcc ttggcacaag ctgagggcac cacgtatccg gagcctgtcc atgcataagt
    11401 agttacaata ttgaggaaag tacccttttg attttcagac aaccatttct tccccattgc
    11461 cagagtcaca ttgtacgatc cccgcaaaac tatatccacc accgtgtcga atgcccgatg
    11521 tgaaagtctt tctgtaggac ttacaaaatt acctgcagca ttgttcagca cagaagttaa
    11581 tttgcctaac tgactttcta tagcttccaa tgatttttca acatcttcat atttccggat
    11641 atcgcaggtg attggcatca cttcatttcc acttgcagaa gacatttcct gcgcggattt
    11701 ttttagtacc tcctcttttc tgcttaagat ggctatcttc gcgccaagcc gcgacatata
    11761 agttgccatc tcttttccca aaccggtgcc tcctccagtt acgagaatta ccttatcttt
    11821 taaagcacct tctttaagca tcccattatc catgattctc ttaattattt gtttagctga
    11881 attaatccca aatattgaac attaatttcg atatttgtca gaacaaataa caaaacatct
    11941 tccatgaatc acagaattat cagtctcgcg gccctggttt taatttttgt atcttcctgc
    12001 attgctccca aaaagtataa tgaacaatta gcggagaacg tcaggatgca aggggaaatt
    12061 acccggctta caagtgattt gagtgtagca aaaaaaagga ttactgattt gaattcaaag
    12121 ctggattcgt tgagtcaaga caacaatatg actaaagata atctggcagc aacatcatct
    12181 tcccttgaaa atcttcaaaa tgaacatgag aaattgcaaa cgctgtacga taatgtgttg
    12241 aagaatagtg gaaaactgaa ccgggatctg gcagagcaac aagaaaggtt gctgacaatg
    12301 gaaagtgatt tggaatctgc caaagcaaag aatgatcaat tgtctattga ccttgctgaa
    12361 agagaaaaga aagtttctga actggaaaaa attcttgctg ataaagaagc cgcggtgctg
    12421 gctcttaaaa agaaagttac agatgcatta ttgaacttcg gtgaaaagga attaactgtg
    12481 gaagtcagaa acggaaaggt ttatgtgagc ctggcggaac aattgctttt caaatccgga
    12541 agcaccagcg tggatgaaaa gggcagaata gcattacaac agttggcagc agcgcttaag
    12601 gattcaaaag atattcatat catggtggaa ggacacaccg acaatgtgcc gataagcaga
    12661 gcatctcaat atatgaatga caactgggat ctgagtgtga tgcgtgcaac tactatcgta
    12721 agaattctaa ccaattatgg tgtaagtcct gagcaagtta ctgcttccgg caaaggagag
    12781 ttttcacccg ttatggaaaa tactactccg gaaaataaac agaaaaatcg tcgcacagag
    12841 atcatcctta caccaaattt ggatgagttg tttaagatat tagaagcgaa ttagtttgat
    12901 aaaatatcgc cctggtaatc aagtatatta aatccagata accttataag gcatgaataa
    12961 acctgatatt agatggaagc agcggttcca taatttcaat gaagcttatc aacggctgaa
    13021 ggaagctgtg gaaattccag aactaaatga actggagcgg aatggactag tacaaaggtt
    13081 tgaatttacc ttagaaatgg gctggaagac attaaaggat tttttacagc acaaagaatt
    13141 ttcgttcaga ccttctccta aagatacact gcgacttgct cagcagagtg gatatattga
    13201 tcaggctcag gagttaatcg atggtcttga actccgaaat atgctgtctc atgattacag
    13261 cggagccaaa tttctggaaa gtgaagagaa atttagaaat ataatttatc cagctttgga
    13321 aaagctgtat ttatttttga aatcagaatt tgaaaagagt gaatgaaaaa cgggctctca
    13381 aaaagagatt atgaaacaat aaaaggaata cttgaaaagt acccggagat aactcttgta
    13441 aatctcttcg gcagccgcgc aacaggcact tttcatgcag gaagcgatat agatctcgca
    13501 ataatgaata agggagttac ggatgaaata atccagagaa tactttccga cttcgaagaa
    13561 agtacattac cttattttgt cgacattatt aattattata ccattgaaca taaaccattc
    13621 cgcgaacata tcgaccgggt tgggcaatta ttttatcaga agaaaatggt aaataaataa
    13681 gttaagtcag ccttttggca ttttcttcgg agatatctgg cccattagaa
//
LOCUS       DASWHX010000231         9478 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_1781401, whole genome shotgun sequence.
ACCESSION   DASWHX010000231 DASWHX010000000
VERSION     DASWHX010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 9478)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9478)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9478
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1781401"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..449)
                     /locus_tag="VGA21_14810"
     CDS             complement(<1..449)
                     /locus_tag="VGA21_14810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="folate hydrolase"
                     /protein_id="HEX9651830.1"
                     /translation="MKNHQLAIFCIVLTLISYKSVSQDRILGFSQASSDAQKALEKKF
                     DEMVTPSNLDQWMKKLAARPHHVGSPYGKENAEFMTALFRQWGFETEIETFQVLFPTP
                     KLRSLEMIAPSVYKAGLEEPALKEDVATNQKKEQLPPYNGYSIDGDVT"
     gene            complement(585..1724)
                     /locus_tag="VGA21_14815"
     CDS             complement(585..1724)
                     /locus_tag="VGA21_14815"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010610001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEX9651831.1"
                     /translation="MKFGTKAIHAGVEPEKVTGAIMTPVFQTSTYVQASPGAHKGFEY
                     SRTQNPTRTALQSNIAALENGTHGLCFASGLSAIDAVIKTLKAGDEVIASNDLYGGTF
                     RLYNKIFSKFGLVFKYTAMDNPEKITPLINDNTKLIWIETPSNPMMNIIDVKAVSEIA
                     RKHGLLLGVDNTFASPYLQNPLDLGADIVMHSVTKYLGGHSDVIMGALVVKDNKLAED
                     LAFVQNSSGAVPGPQDCFLVLRGIKTLHLRMQRHCENGAIVANYLKRHSKVEKVYWPG
                     FEDHPNHSVAKKQMLDFGGMISFLVKGGSKEKTMEVCERFKIFSLAESLGGVESLANH
                     PAIMTHASVAPEERKRIGIHDNLIRLSVGIEDSDDLVEDLDQALS"
     gene            complement(1760..2869)
                     /locus_tag="VGA21_14820"
     CDS             complement(1760..2869)
                     /locus_tag="VGA21_14820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651832.1"
                     /translation="MAVKLFRKYFLPGLIFQSVLIGGAYGTGRELVEFFLSQGPIPGF
                     IGMLFVILLWGILLAISFELARSAQKYDYRSFIESLLGKGWVAYEILYVATMILVISV
                     VASASSELITGYFGWHPLVGSFFILTGVGILAFYGGKTVENFLSAWSFVLYGVYIILI
                     IQIAWQFHPQISRSLETVTEDNHWFVNALRYTGYNIAFVPALLFCLKDIETRKEAISA
                     GFIGGLIGMIPAILFYFAMLSQYPEILSATVPATALLDSLDQPVFSAVFQVVLLGTFL
                     ETGIGFVHGFNQRVAGVIETGGKTMKRRTRAFNAVIILVISIFMANQFGLVNLIAKGY
                     GIITWGFWIVFLLPLLTYGLYKILKTWRNFSSNSN"
     gene            3044..3241
                     /locus_tag="VGA21_14825"
     CDS             3044..3241
                     /locus_tag="VGA21_14825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651833.1"
                     /translation="MKKYHIIVALACAVLAVFYIKTELFLSGVGSIIGTNIQIASIHV
                     NYITGVLAVIIIAYLIRKQES"
     gene            3281..3820
                     /locus_tag="VGA21_14830"
     CDS             3281..3820
                     /locus_tag="VGA21_14830"
                     /inference="COORDINATES: protein motif:HMM:NF019320.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme NO-binding domain-containing protein"
                     /protein_id="HEX9651834.1"
                     /translation="MKGIIFNITEKFINDTYGEDTFDEILVECQLITKEPFVDPGTYP
                     DEDLLEIVGKSCAHLNERLPDFLKKLGKYAFFQLASAYPGFVKGFNHPKDFLKTVDSI
                     IHVEIRKLYRDAYLPEFVYHDPDPLTLVISYYSKRRLYDLMEGLIEGVGEYFNHPISQ
                     QRKLRTVGEREVCDYHLTF"
     gene            3824..4714
                     /locus_tag="VGA21_14835"
     CDS             3824..4714
                     /locus_tag="VGA21_14835"
                     /inference="COORDINATES: protein motif:HMM:NF025854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9651835.1"
                     /translation="MSNQSNTQIAEIRCLKRLQIAQQRIHILQKMIEDQTRELYYKNV
                     ELERIIEVRTQELLSKNLMLQQRNEELMEISYVASHDLQEPLKTISGFVDLFRNQFMN
                     KIDTSADLYIKYITEATSRLQNLIKDLLDFSRIGKAQSLEEIDCNKLISGLLLDLSSA
                     IQESEAVIQTFDLPVIVGSNTELRLLFQNLISNAIKFRQKDNPPEISISCSNSGNELM
                     KFEVADNGIGIESKFKEKIFVLFQRLHNRNEYQGTGIGLAHCKKIVERHGGTIWVDST
                     PGIGSTFYFTIKSNHNEKKA"
     gene            4698..5129
                     /locus_tag="VGA21_14840"
     CDS             4698..5129
                     /locus_tag="VGA21_14840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020533064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9651836.1"
                     /translation="MKRKLECILLIDDDEPTNFLHKLVITNANVAESIKELSSAEDAL
                     TLLASGGSDLSKTEPSLIFLDINMPGMNGWDFLEEYKKLNFVNQGKVIIIMLTTSLNP
                     DDRAKAESNPFIRGFINKPLNSDSLYSILKTHFPEYYEDVA"
     gene            complement(5180..7921)
                     /locus_tag="VGA21_14845"
     CDS             complement(5180..7921)
                     /locus_tag="VGA21_14845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651837.1"
                     /translation="MKVKKYLLYFFLSIASLTVGYFLYIKWFSNNHRTLWDLVPENAL
                     IVYETNGIALIWNDLIQTKQWENLRKIPAVASREFVVEKLDSLAGRQGKLDQISRNDK
                     VLISIHQISNTELDAIFYISLLEENAQKLLREVLDTLSGNDDFEIIERYYQENLIREI
                     KKKSVSRSIFTFSIIDNIFVASFTPFLLEDVVRNISANNNFSNGNENLFSISSLSNDK
                     GNLYINFPAATGYVKNFFSKDNPFSSSLNNLLSNGFLDITFDDRNFFLNGFSFLQKET
                     SLLSTLAEATPGESKVKALIPSQTAMLYQLNVGDGNSWMTGLETFWNNSKSLAGDWEN
                     LQKDYGFQTDRFFSLVTGEIALCLLESLIKQKSELILFIHTSDINGAYNQINTLAERA
                     NKIIGDTLYTETFSDYKITQLNIKQFPQKVLGKQFIGFNEVYYMPVLDYIVFSNSVEN
                     LKSLIIDIESENTWGKRVSQTSFLENTLEKFNAGLIIDIPKAFDLLHNVSSPQLKNFL
                     EKNSRVIKSFEKASIQFRNQGTMLYTSIAIRNGGSIDSKPEVDFEPVQSVAISSEIIS
                     KPFVVRNYINGSLEVLVQDTARFLYLINNQGEVLWKDSLGFNLRGEVEQIDFFKNDKL
                     QYLVCLGDHFRLIDRNGNEITDFTLKLPFQVFNWKVIDYDRSKNYRILISDFQGNLFL
                     YDKNGENLEGWTPKLLSGRLGSVPFHLRVRGRDVIIGIQENGIVNMMTRRGENLRGFP
                     LDLKGRTNNTLFIEKGGDFQSSLVTALTNEGELIKFDLEGKILDRDQLFKPNPTTTFA
                     MVVERLGRTFVIMQQDGSRLSMLDKNGVLLFEKDYLSTTALSGQYYDFGSGKKIYIVI
                     DPIQEFAYIYLENGVLVNSRPLASGNLVGLLYFEGLQKFHVYSTFMNNFQISGFYVQ"
     gene            complement(7962..8531)
                     /locus_tag="VGA21_14850"
     CDS             complement(7962..8531)
                     /locus_tag="VGA21_14850"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX9651838.1"
                     /translation="MGLLSITTVTSIGIYHMWQDNNVKPEISGRETIKSDQVLDENTG
                     SNEVPAQKIEEFSPETNNSNSENVKNFEGRRVVPGEIIRLETINFDENKSVITEDSFE
                     ELNRLAEFLKKNPYVKIEVHGHTDKTKNENFSLELSLKRAESVRKFLITKGVKNKIDV
                     KGYGFDQPLKNTEGVYPTNRRVEVRIIGI"
     gene            complement(9127..9200)
                     /locus_tag="VGA21_14855"
     tRNA            complement(9127..9200)
                     /locus_tag="VGA21_14855"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9164..9166),aa:Ala,seq:tgc)
     gene            complement(9378..>9478)
                     /locus_tag="VGA21_14860"
     rRNA            complement(9378..>9478)
                     /locus_tag="VGA21_14860"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         2977 a         1851 c         1689 g         2961 t
ORIGIN      
        1 gttacatcgc catcaattga atagccattg taaggtggta attgttcttt cttttgatta
       61 gttgctacat cttctttcaa tgcaggttcc tccaatcctg ctttgtaaac ggatggggcg
      121 atcatttcca gacttctcaa tttgggagtt ggaaataaaa cctgaaaagt ttcaatttca
      181 gtttcaaaac cccattgcct gaaaagggct gtcataaatt ccgcattttc tttcccgtac
      241 ggagacccga catgatgagg tcttgcagca agtttcttca tccactgatc taaatttgac
      301 ggcgtcacca tttcatcaaa cttcttttcc aatgcttttt gagcatctga tgacgcctga
      361 ctaaatccta aaattctatc ctgcgaaact gatttataac tgattagagt gaggacaatg
      421 caaaagattg ccaattgatg gtttttcata attagtatat agagttaggg atgcctaaac
      481 taggaaaaaa atcaattttg tcctattcag aaaatgaaag atggttagaa gatttaccgg
      541 aggaaaaaag gttatttgaa tgggaaatcc ggcctcatca gatactatga caaagcctga
      601 tccagatctt ctaccaaatc atccgaatct tcaatgccaa cacttaatct aatcaggttg
      661 tcatgaatgc caattctttt acgctcttcc ggtgcaacgc ttgcgtgggt cattattgcc
      721 gggtgatttg ctaatgactc aactccaccc agggattctg ccagggagaa gattttaaac
      781 cgctcacaaa cttccatagt tttttctttt gaacctcctt tgactaaaaa ggaaatcatt
      841 cctccaaaat ccagcatttg tttcttcgcg acactatgat tgggatgatc ttcaaatcca
      901 ggccaataaa ctttctccac ctttgaatgt ctctttaagt aattggccac aattgctcca
      961 ttttcacaat gtctttgcat tcgtaagtga agcgttttga tacccctcaa tactaaaaaa
     1021 caatcctgag ggcctggaac agctcctgaa ctattctgaa caaaagctaa atcttccgcg
     1081 agcttattat ccttgaccac caatgcgccc atgataacat cagagtgtcc gcctaaatat
     1141 ttggtcaccg aatgcatcac gatatccgcc cccaggtcga gcgggttttg aaggtaagga
     1201 gaagcgaaag tattatccac acccagcaat aatccatgtt tcctggctat ttcagaaact
     1261 gcttttacat caatgatgtt catcattggg ttactgggtg tttcaatcca gatcagtttg
     1321 gtgttatcat ttatcaatgg cgttattttc tcaggattat ccatggcagt atacttgaac
     1381 actaaaccaa acttgctgaa aatcttattg tacaaccgaa atgtgcctcc gtacaaatca
     1441 ttggatgcaa ttacctcatc tccggctttc agagttttaa ttacagcatc aatagctgaa
     1501 agtccggaag cgaagcataa cccatgagta ccattttcca gagctgcaat gtttgattgc
     1561 aaggcggtcc gtgtaggatt ttgtgttctt gaatattcaa atcctttatg agctccgggt
     1621 gaagcttgaa cgtacgttga ggtttgaaac acaggagtca taatcgctcc tgttactttt
     1681 tccggttcaa caccggcatg gattgccttg gttccaaatt tcataatacg aattatttgt
     1741 gactaagata agaaaaatat taatttgaat tgcttgaaaa atttctccag gttttcagga
     1801 ttttatataa gccgtaggtg agtaatggaa gaagaaatac aatccagaac ccccatgtta
     1861 taatgccata gcctttggcg ataaggttca ccaatccaaa ttggtttgcc atgaatatgg
     1921 atataaccag tataattacg gcattaaatg cccgggtcct tcttttcatt gttttacccc
     1981 cggtctcaat aacgccggca actctttgat tgaatccatg cacgaaccca atgcctgttt
     2041 ccaaaaaggt tcctagcaaa actacttgaa aaacagctga gaataccggt tggtcaagag
     2101 aatccaacaa ggcagtggca ggaactgtag ccgataaaat ttctggatat tgactaagca
     2161 ttgcgaaata aaaaagtatg gcaggaatca ttccaataag tcctccaata aaacccgcag
     2221 aaattgcctc ttttcttgtc tcaatatctt ttaaacagaa gagcaaagca ggcacgaaag
     2281 caatattata ccctgtatat ctcaatgcat ttacaaacca atgattgtct tctgtaactg
     2341 tttctaaaga acggctgatt tgtggatgaa actgccaggc gatctggata atcagaataa
     2401 tataaactcc ataaagaaca aaagaccagg ccgataaaaa attttccaca gttttaccgc
     2461 catagaatgc cagaattcca acgcctgtta atataaaaaa acttccaact aatgggtgcc
     2521 agccaaaata tccggtaatt aattcggagg atgcagaggc taccactgag attacaagaa
     2581 tcatggttgc aacgtagaga atttcatagg ctacccaccc ctttccaagt aaactctcaa
     2641 tgaaggaacg gtagtcgtac ttttgagcag atcgagccaa ttcaaaacta atggccaata
     2701 ggattcccca aagcagtatt acaaagagca tcccaataaa gccaggtata ggcccctgac
     2761 taagaaagaa ttcaactaat tccctgcctg taccgtaagc tccacctatt aataccgatt
     2821 ggaagataag tccaggcaaa aagtactttc gaaataattt cacagccaca attacgtcaa
     2881 ttgggggaaa tcctcaaaaa tgtagagctt ataaggatgt ggcaactaat taacgaatta
     2941 aaggggaagt gtttggtaat tttttaccag ataataatat tattatacaa ataatatttt
     3001 gttcttttta cctatttttg atattaaata taactataca actatgaaga agtaccatat
     3061 tattgttgcc ctggcctgcg cggttctagc tgttttttac attaagactg aattattcct
     3121 atccggagtt ggttcaatta tcggaacgaa tattcaaatc gcatcaattc atgtgaatta
     3181 cataactggt gtattggccg taattattat tgcttattta ataagaaagc aggaatctta
     3241 attttaataa ggaattcttt aatcattcac atgtcctgaa atgaaaggaa ttattttcaa
     3301 cataacagaa aaattcatca acgataccta cggggaggat actttcgatg aaatcctggt
     3361 tgagtgccaa ctcattacca aagagccatt tgtagatccg ggaacttatc ctgatgaaga
     3421 ccttctggaa attgtgggca agtcttgcgc tcatttgaat gaaaggttac cagatttcct
     3481 caagaagtta ggaaagtatg ctttttttca gcttgcaagt gcataccccg gttttgttaa
     3541 aggtttcaat catccaaaag atttccttaa aaccgtggat agcattattc atgttgagat
     3601 aagaaagctt taccgggatg cttaccttcc tgaatttgtt tatcacgatc cagatccatt
     3661 aacgttagtt ataagttatt attcgaagag aagactttat gatttgatgg aaggtttaat
     3721 tgaaggagtg ggagaatatt ttaatcatcc aatatcgcaa cagagaaaac taaggaccgt
     3781 tggtgaaaga gaagtctgcg attatcactt aaccttctga ttagtgtcaa atcaaagcaa
     3841 tactcagatc gccgaaatac ggtgtttaaa aagacttcaa attgctcaac aaagaattca
     3901 tattcttcaa aagatgattg aagaccaaac ccgggaactt tattacaaga atgtagaatt
     3961 agaaaggatt attgaagtta gaactcaaga gctactgtca aaaaatttaa tgcttcagca
     4021 gcgaaatgag gaattaatgg aaatatctta cgttgcttcc cacgatttac aggaaccact
     4081 taagaccatc tcagggtttg tagatttgtt tagaaaccaa tttatgaaca agatagatac
     4141 aagtgcagat ttgtacatca aatatataac cgaagcaaca tcgcgattac aaaacctgat
     4201 taaggatttg ttggattttt caagaattgg aaaagcacaa agtctggaag aaattgattg
     4261 taataaactt atctccggct tactcctgga cctgagttcc gccattcagg aaagcgaagc
     4321 agttatacag acatttgatt tacccgtaat agtgggttcg aatacagagc tgcgtttgct
     4381 gttccaaaac ctgataagta acgcgatcaa gtttcgccaa aaggataacc ctccggaaat
     4441 ttcgattagc tgtagtaatt ccggcaatga gcttatgaaa tttgaagtcg cagataacgg
     4501 aattggcatt gaatccaaat tcaaagaaaa gatttttgtt ttatttcagc ggttgcataa
     4561 cagaaatgaa taccaaggta ccggaatagg acttgctcac tgtaaaaaga ttgtggagag
     4621 acatggcggg actatttggg ttgattcaac acctggaatt ggaagtacat tttattttac
     4681 aataaaatca aatcataatg aaaagaaagc ttgaatgcat tttattaatt gatgatgatg
     4741 aaccaactaa ttttctacac aaattggtaa ttaccaatgc gaacgttgca gaaagtataa
     4801 aggaattgag tagcgcggag gacgcattga ccctcctggc ttcgggaggt tcagatctat
     4861 ccaaaaccga accaagccta atatttcttg atataaatat gcctggaatg aatggctggg
     4921 attttctgga agaatataag aaactcaatt tcgtaaatca gggaaaagta attataatca
     4981 tgttgacaac atctttaaac cccgatgacc gggcaaaggc agaatccaac ccattcattc
     5041 gtggctttat caacaaacca cttaattctg attctttgta ttcaattctg aaaacacatt
     5101 ttccggaata ttatgaggac gttgcctgat tccagatcca ctacttaatg gaaatcgtta
     5161 tccgcatgat tttttggttt tactggacat aaaaacctga aatctgaaag ttattcataa
     5221 aggtggaata gacatggaac ttttgaagcc cttcgaaata taataagccc accaaattgc
     5281 cactggcaag cggtcttgaa ttaacaagga caccattttc aagatagatg taggcaaatt
     5341 cctgaatagg gtcaatcaca atataaattt ttttacccga accaaaatca taatattgac
     5401 cagacagagc agttgtacta agataatctt tctcaaataa aagcactccg tttttatcca
     5461 gcatactaag tctggagcca tcctgctgca ttattacaaa tgtacgaccc agcctttcta
     5521 caaccatagc aaaagtggtg gttggattgg gtttgaataa ctgatcacgg tctaagatct
     5581 tcccttctag gtcaaatttt atcagctcac cttcattggt aagcgcggtt accaatgagg
     5641 actgaaaatc tccacccttt tcaataaata gagtattgtt tgttcttccc tttaaatcca
     5701 aagggaaccc gcggaggttt tcacctctgc gggtcatcat atttacaatt ccattttcct
     5761 gaattccaat tattacgtct ctgcctctta ctcgcagatg aaaaggtact gatcctaatc
     5821 tcccggacaa taatttggga gtccatcctt cgaggttttc accattctta tcatatagaa
     5881 ataagttacc ctgaaaatcc gaaataagaa ttctgtaatt tttacttctg tcataatcga
     5941 ttactttcca attaaagacc tgaaatggaa gcttgagggt aaagtccgtg atttcattcc
     6001 catttctgtc aataagcctg aaatgatcgc ccaaacacac aagatattgt aatttgtcat
     6061 ttttgaaaaa atctatttgc tcaacttcac cgcgtaaatt aaatcccaaa gaatctttcc
     6121 acagaacctc accctgatta ttaatcaggt atagaaacct tgctgtatcc tgtactaaaa
     6181 cttcaagcga tccattaata tagtttcgca caacaaaagg ctttgaaata atttcagaag
     6241 aaattgcaac tgattgcaca ggctcgaagt caacttccgg ttttgaatct atgctcccgc
     6301 cgttgcggat tgcaatggaa gtgtataaca ttgttccctg gttcctaaat tgaatactgg
     6361 ctttttcaaa acttttgatt actctggaat ttttctcaag gaaatttttt aactggggtg
     6421 aacttacatt gtgcaataaa tcgaatgcct taggaatatc tatgattagg ccggcgttaa
     6481 atttctcaag agtgttttca aggaagctgg tttgacttac tcgcttaccc caggtatttt
     6541 cagactcaat atctattatt agagatttga gattttccac gctgttggag aacactatgt
     6601 aatccaatac gggcatataa taaacctcat taaagccaat gaattgtttt cctaaaacct
     6661 tttggggaaa ctgctttatg tttaactgag taatcttata atcgctgaat gtctcggtat
     6721 ataaagtatc cccgataatc ttattagccc tttctgcaag ggtatttatc tgattataag
     6781 caccattaat atcgcttgta tgaataaaca agatcaattc actcttttgc ttaattaaag
     6841 actcaagcag acataaggca atttctcctg ttacaagaga aaagaatcta tccgtttgga
     6901 aaccgtaatc tttttgcaaa ttttcccaat cgcccgctaa tgatttgctg ttgttccaaa
     6961 aggtttccaa gccagtcatc caggagtttc catcaccaac gtttaactgg taaagcattg
     7021 ctgtttgaga aggaatcagg gctttaactt ttgattcgcc tggcgtggct tcggctaagg
     7081 ttgataataa cgaagtttct ttttgcagaa aactgaaccc attcaaaaaa aagtttctat
     7141 catcaaatgt gatatctaaa aaaccatttg acagaagatt atttagtgag ctactaaacg
     7201 gattgtcttt tgagaaaaag tttttaacat agccggtcgc tgcaggaaag ttaatgtata
     7261 aattaccctt atcgttactt aggctggata ttgaaaatag attttcatta ccatttgaga
     7321 aattattgtt tgcgctaata tttcttacaa catcttctaa aaggaaaggt gtaaaacttg
     7381 caacgaatat gttgtcgatt atgctaaaag taaaaattga cctggaaacg gacttctttt
     7441 ttatttcacg aatcaggttt tcctgatagt atcgttcaat gatttcgaag tcatcattgc
     7501 cggataatgt atcaagtact tctctgagga gtttttgtgc attttcttcc agtagcgaga
     7561 tatagaatat agcatctagt tccgtattgc taatttgatg aatcgaaatt aatactttgt
     7621 cattgcggct aatttggtct agttttccct gccttccggc taaactatcc agcttttcaa
     7681 ccacaaactc cctggacgca actgctggaa tttttcgcaa attctcccat tgcttagtct
     7741 gaatcaggtc attccaaata agagcaattc catttgtttc ataaactatg agggcatttt
     7801 ccggaaccag atcccataaa gttctatggt tattggaaaa ccatttgata tataagaaat
     7861 agccaactgt taatgaagca atgcttagaa agaaatacaa taaatatttc ttcactttca
     7921 ttcttttcca gcaaaggtaa aattaattgg caaacatgta atcaaattcc gataatacgg
     7981 acttctactc gtcgattggt tggataaaca ccttccgtat ttttcaacgg ttggtcaaaa
     8041 ccgtaccctt tgacatcaat cttatttttt acaccttttg taattaaaaa cttccttacg
     8101 gattctgccc ttttaagaga taattccaaa ctaaagtttt catttttggt tttgtctgta
     8161 tgtccatgaa cttctatttt gacataagga ttttttttca aaaattccgc aagcctgttt
     8221 agctcttcaa atgaatcttc agttatgaca gatttatttt catcaaaatt tatcgtctca
     8281 agtctaataa tttctcctgg aactactcgt ctgccctcaa agtttttgac gttttcagag
     8341 ttcgaattat tagtttcggg agaaaactct tcgatttttt gagcaggaac ttcattggaa
     8401 cccgtgtttt catccaatac ttgatccgat ttgattgttt cccgaccaga gatttcaggt
     8461 ttgacattat tgtcttgcca catatggtaa attcctatgg acgtaactgt ggtaatagaa
     8521 agcaaaccca ttaagattat cagacctctg gtcttatttc gtaatttcct ttcaaagcta
     8581 tacttcataa gagtttcttt tttggaatac tgaaaaatac caggttaatt ggtaattttc
     8641 ctttgtttga accaggcttc accactcatg tacttttcag ggataagtgg gtaatagatt
     8701 attcgttctt tctttattta acgctttatt gctatttaga agacttccag agtgagagta
     8761 atatttattg tgtttaaatt aaattactga taatttctta gaaatacaat aattttttaa
     8821 ggaattactt tcattttacc cattaggaat caaattgtat tatttgaaaa tgagcaaatt
     8881 tggaaccgaa ttattcaaaa tttgttccca tgtcggtctg gaattaccgg cataaagttt
     8941 tgtcgaattt ataaagattt tgggaaatcg aaattgtcat taaaacagac caaaaaagtg
     9001 gccatcggac ccctactaat atcccaactt tctatggtgg agaataccgg aaaccttccc
     9061 ggtgaaatac cgggacgctc cagttcatgc attactttcc cctactcata tcccaacttt
     9121 ctatagtgga gaataccgga atcgaaccgg tgacctcctg cttgcaaggc aggcgctcta
     9181 gccagctgag ctaatccccc agttaattgc attattgcta attgataatg tgagctacaa
     9241 gccccagttt aggtatgagt ggagagcttg gagcttagag agttctcttt taactctgta
     9301 ctctcaactc actctgttct tgccttacaa taccgtaaaa caattgaaaa tgataacaaa
     9361 ccaggcccgg gctccagaaa ggaggtgttc cagccacacc ttccggtacg gctaccttgt
     9421 tacgacttag ccctagtcac tggtataacc cttaacagct cctgtaacgg tcaccatc
//
LOCUS       DASWHX010000232         2587 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_1034239, whole genome shotgun sequence.
ACCESSION   DASWHX010000232 DASWHX010000000
VERSION     DASWHX010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 2587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2587
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1034239"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1885
                     /locus_tag="VGA21_14865"
     CDS             <1..1885
                     /locus_tag="VGA21_14865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018618950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transferrin receptor-like dimerization
                     domain-containing protein"
                     /protein_id="HEX9651839.1"
                     /translation="LRSLEMIAPSVYKAGLEEPALKEDVATNQKKEQLPPYNGYSIDG
                     DVTGDLVFVNYGVPEDYEVLKRMGVEVKGKIVIAKYFGSWRGIKPKVAAENGAIGCII
                     YSDPKEDGYYQGDVYPKGAYKNEYGVQRGSVMDMPLYPGDPLTPNIGATADAKRLDYK
                     DAPTITKIPVLPISYKDAEPLLAALGGQVVPNEWKGALPITYHIGPGPAKVRLRLQFN
                     WDLKPAYNVIARMRGTEAPDQWVIRGNHHDAWVNGANDPISGMVALMEEARVVGELAK
                     NGWKPKRTIIYCAWDAEEPGLIGSTEWVEFHAEELKDKGAIYLNTDSNARGFLGAGGS
                     HTLEKFFQEIANDVTDPQTGLTVAERRKAAMKTYGNPDYEKFTLYALGSGSDYTPFIQ
                     HLGIASLNVGFGGESGGGEYHSIYDTYDHYKRFKDPGFAYGETLAKVMGRATLRLANT
                     EILPFEFGSLTTTVDSYLKEVMNIADKMRSETSRHNQNVEKGYYKAAADPTLPFVEPE
                     MKNVVPYFDFSGLQNEMTNLKNVVQKVDSKTFSMEPSSKLNKLLYQSERKLTLDEGLP
                     RRPWFKHHIYAPGFYTGYGVKTLPGVREAIEQRDWEEVRKEIDDLEKVINQLSMYLES
                     IIK"
     gene            1926..>2587
                     /locus_tag="VGA21_14870"
     CDS             1926..>2587
                     /locus_tag="VGA21_14870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013454978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEX9651840.1"
                     /translation="MKKYLGLLIVLSLWTSPDILSQRRGGAGASSTTTYDEKLYNALE
                     WRSIGPFRGGRSAAVTGVPGKTNLFYFGSTGGGVWRTRDGGQSWEIISDGYFGGSIGV
                     IAVSDWDNNVMYVGGGEKTVRGNVSYGSGMWKSLDAGKTWKSLGLKESRHIARIRIHP
                     KNPDLVYAAIMGDLFKSSEMRGVYRSKDGGNTWERILFANADAGAVDLIMDPNNPRIL
                     YA"
BASE COUNT          821 a          447 c          631 g          688 t
ORIGIN      
        1 attgagaagt ctggaaatga tcgccccatc cgtttacaaa gcaggattgg aggaacctgc
       61 attgaaagaa gatgtagcaa ctaatcaaaa gaaagaacaa ttaccacctt acaatggcta
      121 ttcaattgat ggcgatgtaa ctggagattt ggttttcgta aattatggag ttcctgaaga
      181 ttatgaagtt ctgaaacgaa tgggtgtaga ggtaaaaggg aaaatcgtaa ttgccaaata
      241 tttcggatcg tggcgaggca tcaagccaaa agttgcggct gaaaatggag caattggttg
      301 tattatctat tcagatccaa aagaagatgg atactaccag ggtgatgttt atccgaaagg
      361 tgcttataaa aatgagtatg gagttcagcg aggttctgta atggatatgc cactttatcc
      421 cggtgatcct ttgaccccga acattggagc aacggcggat gcaaagagac tggattacaa
      481 agatgcacca acgataacca aaataccggt acttccaatt tcctataaag atgccgaacc
      541 actattggca gcgcttggtg gtcaggtagt gccgaatgaa tggaaaggag ccttacctat
      601 aacttatcat attggtccgg gtccggcaaa agttaggttg agactccagt ttaattggga
      661 tttaaaaccc gcctataatg ttattgccag gatgcgagga actgaagccc ctgatcaatg
      721 ggtgatcagg ggtaaccacc atgatgcgtg ggtcaatggc gctaatgatc ctatatcagg
      781 aatggtggcc ttaatggaag aagcaagagt ggtgggtgaa ttggcaaaaa atgggtggaa
      841 accaaaacga actattattt attgtgcatg ggatgcggag gagccgggtt taataggttc
      901 gacagaatgg gttgaatttc atgctgaaga attgaaagat aaaggcgcga tttacttaaa
      961 tactgattcc aacgcccgag ggtttctggg cgcgggaggc tcgcatactc tggagaaatt
     1021 tttccaggaa attgccaatg atgttaccga tccacaaacc gggttaaccg ttgctgaaag
     1081 aagaaaagct gcgatgaaga cttatgggaa tcctgattat gaaaaattta ctttgtatgc
     1141 gttgggctca ggatctgatt acactccttt tatacagcat ttgggaattg caagtttgaa
     1201 cgttggattt ggtggggaaa gtggtggcgg ggagtatcat agtatttatg acacatacga
     1261 tcattacaaa cgatttaaag atcctggatt tgcctacggt gaaacattgg ccaaagtaat
     1321 gggacgggct acccttcgtc ttgctaatac tgaaattctt ccttttgaat tcgggagttt
     1381 aacaacaacc gttgatagtt accttaaaga agtaatgaac attgccgata agatgagatc
     1441 tgaaacttcc cgacacaatc aaaatgttga aaaaggatat tacaaagctg ccgctgaccc
     1501 aactttacct tttgtagagc cggaaatgaa aaatgtagta ccttattttg atttctcagg
     1561 tcttcaaaat gaaatgacaa atctgaaaaa tgttgttcaa aaagttgatt caaaaacttt
     1621 ctccatggag ccatcatcaa aactcaacaa attactttat caaagtgaaa gaaaacttac
     1681 tttggatgaa ggtctgcccc gtcgtccatg gtttaaacat catatatatg ctcccgggtt
     1741 ttatacaggt tatggagtaa aaacactacc tggggtccgg gaagcgattg agcaaagaga
     1801 ttgggaagaa gttcgaaaag aaattgatga ccttgaaaaa gtgataaatc aattatcgat
     1861 gtatcttgaa agtattatta agtaaaatat cctaatgaaa tcttataact aaaatctgac
     1921 cagccatgaa aaaatatctt ggattattga ttgtcttgtc attatggaca agtcctgaca
     1981 ttttatccca gcgaagggga ggtgcaggag catcatcaac aacaacctat gatgaaaaat
     2041 tgtacaacgc acttgaatgg cgaagcattg gacctttcag aggaggaaga tcagcggcag
     2101 ttacgggcgt gcccggaaaa acaaatttgt tttattttgg ctctacagga ggcggggtct
     2161 ggagaacccg ggatggcggt caaagttggg aaattatctc cgatggatat tttggtggat
     2221 ccatcggcgt aattgcagtg agtgattggg ataacaatgt gatgtatgtt ggtggaggtg
     2281 aaaaaaccgt tcgcggaaat gtttcttacg gatctggaat gtggaaatca ttagacgcag
     2341 gaaaaacctg gaaaagctta gggctgaagg aatcccggca tattgccaga atcagaattc
     2401 atcctaaaaa tcctgacctt gtctatgctg ccattatggg agatttattt aaatcttcag
     2461 aaatgcgggg cgtttatcgc agtaaagatg gcggcaacac ctgggagcga atcttgtttg
     2521 ccaatgcaga tgcgggagcc gttgacctaa ttatggaccc caacaacccc agaattttat
     2581 atgcaag
//
LOCUS       DASWHX010000233         1814 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_284927, whole genome shotgun sequence.
ACCESSION   DASWHX010000233 DASWHX010000000
VERSION     DASWHX010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 1814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1814
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_284927"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..103
                     /locus_tag="VGA21_14875"
     CDS             <1..103
                     /locus_tag="VGA21_14875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018475241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bL21 family ribosomal protein"
                     /protein_id="HEX9651841.1"
                     /translation="DKVIIFKKKRRKGYKLKNGHRQRLTQLLIKKIS"
     gene            107..370
                     /gene="rpmA"
                     /locus_tag="VGA21_14880"
     CDS             107..370
                     /gene="rpmA"
                     /locus_tag="VGA21_14880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020527948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEX9651842.1"
                     /translation="MAHKKGAGSSRNGRESHSKRLGVKIYGGQEVKAGNIIVRQRGTS
                     HHPGPNVGLGKDHTLFALTDGVVTFRKGRKDRSYVSVSTSEKK"
     gene            617..>1814
                     /locus_tag="VGA21_14885"
     CDS             617..>1814
                     /locus_tag="VGA21_14885"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEX9651843.1"
                     /translation="MKQKLLIFLSILLSGLVAQGQGLTTSSMNGRVNDSAGEPLPGAT
                     IVAIHNPTGTQFGNITNADGFFRIPNMTVGGPYTITISFVGYQDFVKQNIYLTLGQTF
                     KLDANMQEQVTELEGIDIIANRNDIFDGNRTGAQTMVGLETIENTPTLGRTIGDFARF
                     LPQARIEEGFDGFEISIAGINNRYNSIYIDGAVSNDVFGLSASGTNGGQTGVSPYPID
                     AIEQFNIVIAPFDVRQSGFAGGSVNAITRSGTNSVQGSAYWYSRNEGLAGKTPAEDFS
                     GGITRDRLPEFSSNIYGVRVGAPIIKDKLFFFLNAEVQRQETPQPYDFNTYIGTATSG
                     DVQNVISVLNGYGYDPGPFDNNTSFLNSEKIIGKFDFNLGKNHKLSLRHSYVNAENLE
                     ARSSSTS"
BASE COUNT          568 a          346 c          402 g          498 t
ORIGIN      
        1 cgataaggtt atcatcttta agaaaaagag aagaaaaggg tataaactta agaacggcca
       61 caggcagcgc ttaacccaac tgttaattaa gaaaatttcg taagaaatgg cgcataaaaa
      121 aggagcaggt agttccagaa atggaagaga atctcatagc aagaggcttg gggttaaaat
      181 ttacggcggt caggaggtaa aggccgggaa tattattgta aggcaaaggg gcacatccca
      241 tcatccgggc cctaacgtag gccttggtaa agatcatact ctttttgcat taactgatgg
      301 agtggttact tttagaaaag gtcggaaaga tcgctcctac gtttccgtct caacttctga
      361 aaagaaataa aaacttctga agagaaaaat aatgagaggc cgtttaaacg gcctttttta
      421 tgcctcggtt ttcctgcagg acatacgatt taaaattcaa gtcaattcac agtttgtgat
      481 tagccaacca tttcctaatt tgtgcctgca ttcagacacc aaaaattaac taaatgataa
      541 tacttgctgt ccatgctaaa ttcaaagtcg acgtatcttt agaggaatct aaattaatca
      601 actgaactac acacccatga aacaaaaatt actaatattc ttaagcattc tgctctcggg
      661 gcttgttgcg caagggcaag gattaaccac ttcctctatg aacggaaggg ttaatgacag
      721 tgccggggaa ccactcccgg gtgcaaccat tgttgcgatt cataatccaa caggaacaca
      781 atttggaaat ataaccaatg cagatggttt cttcaggatt cccaatatga ctgtgggcgg
      841 tccctatacc attactataa gctttgttgg ttatcaggac tttgttaaac aaaacattta
      901 tttaacactt ggtcaaactt tcaagcttga tgcgaacatg caggagcagg ttactgagct
      961 tgaagggatt gacattattg ctaacaggaa tgatattttt gatggtaacc ggaccggcgc
     1021 tcaaaccatg gtgggtcttg aaactataga aaacacccca acgctgggac gaaccattgg
     1081 tgattttgcc cgatttttgc cccaggccag aattgaagaa ggctttgatg ggtttgaaat
     1141 ttctattgcc gggatcaata acaggtataa ctccatctac atcgatgggg cagtaagcaa
     1201 tgacgtgttt gggttatctg ccagtggaac caacgggggg cagacgggtg tatctcctta
     1261 tccaattgat gcgattgagc agtttaacat tgtgattgca ccgttcgatg taaggcaaag
     1321 tggatttgca ggtggttcgg taaacgcaat caccagaagt ggaacaaact cagtacaagg
     1381 atctgcttat tggtattcaa gaaatgaagg tttggccgga aagacgccgg ctgaagattt
     1441 ttctggcgga attaccagag atagattacc ggaattctcc agcaatattt atggcgtaag
     1501 agttggggct ccaattatca aggacaaatt gtttttcttt ttaaatgctg aagttcagag
     1561 gcaggaaact cctcaaccct atgacttcaa tacatatata ggtactgcca caagcggcga
     1621 tgttcaaaat gttattagtg ttttgaatgg ctatggatat gatcccggcc cttttgataa
     1681 caatacttcg ttccttaata gtgaaaagat aattggcaaa tttgatttca acctgggcaa
     1741 aaaccacaag ctttctctaa ggcattctta tgttaatgcg gaaaaccttg aagcaagaag
     1801 ctcatctacc tcta
//
LOCUS       DASWHX010000234         2886 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_3591314, whole genome shotgun sequence.
ACCESSION   DASWHX010000234 DASWHX010000000
VERSION     DASWHX010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 2886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2886
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3591314"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..700)
                     /locus_tag="VGA21_14890"
     CDS             complement(<1..700)
                     /locus_tag="VGA21_14890"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HEX9651844.1"
                     /translation="MNKSMTLILAIVWLSGCASYKSNVMFSGAGSGNDNAWFEMSSFP
                     IEVNDQIEINVLPWKGEELMIGNEEIMAQHIKITIDSKGYAQIPLLGKIFLQGYNLAE
                     ADSLLSLSFANFLTEPFVETRFLNKRVSVIGAEKTQVVELPSQTIRLSEVIALSGGIP
                     SKSRANNIKILREDRYFIADLSRAKERLKNDIYIYPGDLIYIEPVKRPFSEGLRDSLP
                     LISTLTSIITLFALV"
     gene            complement(731..1876)
                     /locus_tag="VGA21_14895"
     CDS             complement(731..1876)
                     /locus_tag="VGA21_14895"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX9651845.1"
                     /translation="MKIAIVLNTSWNIYNFRKGLIKNFLEQGHEVVAIAPKDQFSDRI
                     RELPCQFIPIRMDSRGINPLKDIGLFFELLWIYYRHAPDLILHYTIKPNIFGSIAAGL
                     LGIKNVANVCGLGTLFLNPGLPTSFAMWLYKLAFRFPFKVFFQNAEDQNVFVSKNLIV
                     ESRCRQIPGSGVDVDFFRPFLKTIQSEVHFLMMSRIILDKGVKEFLSAARSLKDKGYN
                     VRFSLAGKLDPYHKRGIPMAKFAEWLDWSGVNYLGFKEDIRHLISTSDVVVLPSYREG
                     TPKSLLEAAACGKPIICTNVPGCKQVVQEGYNGFLCQPGSAADLERAMKQFYDLELPE
                     RRQMGRNSRKVAETKFSERIVIGIYNEIIQELDLPAPEFSEPQMQRI"
     gene            2043..2669
                     /locus_tag="VGA21_14900"
     CDS             2043..2669
                     /locus_tag="VGA21_14900"
                     /inference="COORDINATES: protein motif:HMM:NF016161.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB2 family protein"
                     /protein_id="HEX9651846.1"
                     /translation="MEIGFLHLHKTVVVIFLLLILSKVVLLLAGKKELLQKLRSKTRV
                     ADMILGGLILITGIYLFFLKANPEVYLYAKIILVLISIPLGIVGFKKQKPILAVMSLF
                     IIIYMYGIAETRSLSFKPPKFDLPAGIKNESSLSRGEVIYQSLCIDCHGSDGKKGLFK
                     APDLTTSKLTLQQRLDVISNGKGLMQPYSRQLNEEEIESVLEYIGSLK"
     gene            2666..>2886
                     /locus_tag="VGA21_14905"
     CDS             2666..>2886
                     /locus_tag="VGA21_14905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017730230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEX9651847.1"
                     /translation="MIKTFETNHRKEKAVLVALINQFQTDEMVREYLDELAFLATTLG
                     VETLGEFTQRLEKPDVATFVRKGKLEEIK"
BASE COUNT          886 a          571 c          542 g          887 t
ORIGIN      
        1 taaccagtgc gaatagggta attatgcttg ttaatgttga aattaacgga agcgaatctc
       61 taaggccttc actgaatggt cttttgaccg gttcaatata gattaagtct cccggataaa
      121 tataaatatc attttttagc ctttcctttg ccctggataa atctgcgata aaatacctgt
      181 cttcccttaa tatcttaatg ttattcgccc tgcttttaga gggtattcct cccgataacg
      241 cgatgacttc tgaaagtctt atcgtttgac tgggtaattc caccacctga gtcttctcag
      301 cgccaataac agagactctt ttgtttaaga aacgtgtctc aacaaagggt tccgttaaaa
      361 agttagcaaa ggataaactt aacaaggagt cggcttcagc aaggttataa ccttggagga
      421 aaatcttacc caataaaggt atctgcgcat atccttttga atcaatagtt atcttgatat
      481 gttgagccat aatttcctca tttcctatca tcaattcttc acccttccag ggtagtacat
      541 tgatttcaat ctgatcattt acttcaatcg gaaagctgct catttcaaac caggcattat
      601 cattaccaga ccccgcgcca ctaaacatta catttgactt gtaactggcg catcctgaca
      661 gccacacgat tgccagaatc agcgtcatac ttttattcat attcatctaa ataatgggga
      721 ctttgacatt ttaaatcctc tgcatttgag gctcgctgaa ttccggagcg gggagatcca
      781 actcttgaat tatttcatta taaattccta taactattct ttcactaaat tttgtttctg
      841 caacttttct tgagtttcgt cccatttgcc ttctttcagg caattcaaga tcataaaatt
      901 gcttcattgc tctttctaaa tccgctgcgc ttcctggctg acataaaaat ccattgtatc
      961 cctcctgcac aacttgttta catccgggaa catttgtgca tattattggc ttaccacaag
     1021 ctgcagcttc cagtaatgat tttggggttc cttcacggta tgagggaagc acgacaacat
     1081 cgctggttga aattaagtgt cttatatctt ccttgaatcc caggtaattt actccggacc
     1141 aatccagcca ctctgcaaat ttcgccattg ggatcccgcg tttgtgataa ggatccagtt
     1201 tacctgccag ggaaaacctg acattatatc ccttatcttt taaggagcgt gcagcggaaa
     1261 gaaattcttt cactccttta tccaggataa ttcttgacat catcaaaaaa tgaacttcac
     1321 tctgaattgt ttttaagaat ggtctaaaga aatcaacatc aacaccagat ccgggtattt
     1381 gcctgcatct ggattcaaca atcaaatttt tggatacaaa aacattctga tcctctgcat
     1441 tctgaaaaaa tactttgaag ggaaaccgga aggctaattt ataaagccac atcgcgaaac
     1501 ttgttggtaa cccagggtta agaaacaggg ttcccagccc acaaacatta gctacatttt
     1561 ttattcctaa taagccggcc gctatgcttc caaaaatatt tggtttgatg gtataatgta
     1621 agatgaggtc cggggcatga cggtaataga tccaaagaag ttcaaaaaac agcccaatat
     1681 cttttaatgg attgatcccc cggctgtcca tccttatggg aatgaactgg caaggcaatt
     1741 ccctgatgcg atcgctgaat tgatctttgg gtgcaatagc aactacttca tgtccctgtt
     1801 ctaaaaaatt cttgattaac cccttccgga aattgtagat attccacgag gtattcaaca
     1861 caattgcaat cttcattgtt acttttaaat cgaaaccaga ttaattaatt gtgcagtaaa
     1921 aagaaggaat tcaagacaaa tataagcgaa taagaaactg aagaagaggt attgtaagat
     1981 atttggtaag attgcatagt tttgaatatg cgggtaaaaa ctaaatttga caaaaattaa
     2041 gtatggaaat aggatttctt caccttcata aaaccgttgt cgtcattttt ttgctattga
     2101 ttctaagtaa ggttgtgctt cttctggccg gaaagaaaga attactgcag aaattaaggt
     2161 ccaaaacccg ggtagcagac atgattttag gcggattaat actaataacc gggatttatt
     2221 tattttttct aaaggctaat cctgaggtgt atttatatgc taaaataatt ttggttctga
     2281 tttccatccc attaggtatc gtggggttca aaaaacaaaa accaatttta gccgtcatgt
     2341 cactttttat cattatctac atgtacggga tcgcggaaac gagaagtctt tcattcaaac
     2401 ctccaaaatt tgatctgccc gcgggaatta aaaatgaatc ttccttatca cgaggtgaag
     2461 taatttacca gagtctttgt atagactgtc acggatcgga tgggaagaag ggtttgttta
     2521 aagctccgga tttaacaaca agcaagctaa ccttgcaaca aagattggat gtaatttcga
     2581 atggaaaagg attaatgcaa ccctattcca ggcaattaaa tgaagaagaa atagaatccg
     2641 tattggaata tattggcagt ttgaagtgat aaaaacattt gaaaccaacc atagaaagga
     2701 aaaggcggta ttagttgcgc ttatcaacca gtttcaaacc gatgagatgg ttagggagta
     2761 tctggatgaa cttgccttcc ttgcgacaac ccttggagtt gagacgttgg gtgaatttac
     2821 ccagaggttg gaaaagcctg atgttgccac cttcgtcagg aaaggtaagc ttgaagaaat
     2881 caagaa
//
LOCUS       DASWHX010000235         1523 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_1037369, whole genome shotgun sequence.
ACCESSION   DASWHX010000235 DASWHX010000000
VERSION     DASWHX010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 1523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1523
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1037369"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(240..1277)
                     /gene="rlmN"
                     /locus_tag="VGA21_14910"
     CDS             complement(240..1277)
                     /gene="rlmN"
                     /locus_tag="VGA21_14910"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019942116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEX9651848.1"
                     /translation="MNEKKDIRRESLEGITNFLTINGEKPYKARQVHEWLWMKSARDF
                     DVMSNLSLATRALLKESYTIRPVEIALKQKSKDYTIKVGFRLHDGNLVEGVLIPSKDR
                     MTACISSQVGCSLSCKFCATGYMERKRNLDAAEIYDQVIRIRDLSEEEFGKPLTNIVY
                     MGMGEPLLNYSNVLESVNHITNEKGLGMSYKRITVSTAGIAKMIRRLGDDEVKFNLAL
                     SLHAANDEKRNKIMSINETNSLKILAESLNYFYSKTENEITFEYIIFNDFNDSQKDAE
                     ELLAFCRKVPSKVNIIEYNPIREADFRNARPDKIENFRAFLEKQGVNVRVRRSRGKDI
                     DAACGQLANKQ"
BASE COUNT          421 a          331 c          282 g          489 t
ORIGIN      
        1 tttgcaggct ttaaaattct taaggaagaa aatccattta taaatcgtat ggaagaaaag
       61 ggagataaag agtggatgat tatcgcgatt cgccccgaat aacggtttac tttttcgatt
      121 caaggtagac tcatattaaa ttagctttca ctccaattac caaaaaacca aatatcccaa
      181 tggcgggaaa agtgtaaata acggtctcag gcttccggag aaccagactc gccacaagtt
      241 cactgtttat tcgccagctg gccgcaagca gcatcaatat ctttaccccg gcttcttcgc
      301 acccgtacat ttacaccttg tttttccaga aaagctctga agttttcgat tttgtctggc
      361 cgtgcattcc tgaaatctgc ttcgcgaatc ggattgtatt caatgatatt caccttagat
      421 ggaaccttcc tgcaaaatgc aaggagttcc tcagcgtctt tctggctgtc attaaaatca
      481 ttgaaaatta tgtattcaaa agttatttca ttctctgttt tcgaataaaa atagttgagt
      541 gattcagcaa gtattttcag ggaattcgtt tcattgatag acattatttt atttcgcttt
      601 tcgtcattgg ccgcatgaag tgataatgcc aggttaaatt ttacttcatc atctccaagt
      661 cgcctgatca tttttgcaat gccggctgta ctcacagtga ttctcttgta cgacatcccc
      721 aacccttttt cgtttgtaat atggtttact gattccagta cattgctata gttcagcagc
      781 ggttctccca ttcccatgta cacaatatta gtaagaggct tgccgaactc ttcttccgac
      841 aggtctctga tccgaatcac ctggtcgtat atttccgctg catccaaatt gcgttttctt
      901 tccatataac cggtggcgca aaatttgcag gaaagcgaac agcctacctg tgatgaaata
      961 caagcagtca tcctgtcttt ggatggtatt aatacgcctt caaccaggtt gccgtcatgc
     1021 agcctgaagc caactttaat ggtgtaatcc ttgctcttct gttttaatgc aatctcaacc
     1081 ggtcgaattg tataactttc tttcagcaaa gcgcgggtgg caagagatag attagacatc
     1141 acatcaaagt cacgcgcaga tttcatccac aaccattcat gaacctgtcg tgctttgtaa
     1201 ggcttttccc cattaatggt gaggaaattc gttatcccct ccaaactttc ccttctaatg
     1261 tcttttttct cattcacatc gcaaaagtaa tgagaatctt tctccttgca tataattcat
     1321 tcccaatgac atattgtcct gcttttttag caaatcatcc atttaatgtc ataatgtcaa
     1381 gtataaattg aaatgatttg gatggattat tttttgatgc cctccattcg gaggatttac
     1441 gaaaaatgaa ttttgataac tatacggtaa aagcgcagga agttgtgcag aaggcggcag
     1501 agagcgtggc gttaaacgga cat
//
LOCUS       DASWHX010000236         9170 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_1049624, whole genome shotgun sequence.
ACCESSION   DASWHX010000236 DASWHX010000000
VERSION     DASWHX010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 9170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9170
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1049624"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..329
                     /locus_tag="VGA21_14915"
     CDS             <1..329
                     /locus_tag="VGA21_14915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651849.1"
                     /translation="LLICGFTILTTSQAHSQAMVTRGQEVQLIWFGGVTAISFHNVQV
                     ETANGSLQFTVSFQLPDGAPGVNKKAYTLPLYWISEFGIHVGSGIVTPNGKVIARFHV
                     KTENRT"
     gene            456..1547
                     /locus_tag="VGA21_14920"
     CDS             456..1547
                     /locus_tag="VGA21_14920"
                     /inference="COORDINATES: protein motif:HMM:NF042644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6588 family protein"
                     /protein_id="HEX9651850.1"
                     /translation="MKNLKVLFLCALFVFPFRALAQEDLIEYLPAGEADAKFLMDQYL
                     SIMAAVVESGLNSGWYTTGDPHYKFGFEINAALNTIFIPSRENFFTVTSSDLGNSSLV
                     KRETDGSYTILTTGEVPTGYGPEDVTPIFQITNPADPNAGAIFKGPSGNDLSDEFLIN
                     AVIVPSLQLGIGIPYNMDIKIRYTPQYDYGDVKFGNWGVGLMHSIKQHIPALKVLPFG
                     WSLFIAYSRLNGEIDFSGQWTGNDQLGEFHSNTWTFQTIASKDFSIFTVYGGIGYNIG
                     NTEFDIKGTYNIVDVNPGDGMRVPLNTPIVADLTSNPYHFAYDSQSLRMTLGLRMKLG
                     PLIFFGDYTLQRTSVLTFGMGFTFKEDEF"
     gene            complement(1556..2281)
                     /locus_tag="VGA21_14925"
     CDS             complement(1556..2281)
                     /locus_tag="VGA21_14925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651851.1"
                     /translation="MNTKPCLFTVFLLILFNATFAQISDFLHHSSFDWSYWEIYDNDQ
                     EKSEFLSKSGLQTMNAATVKIVDIDGNQIPDVISISGGNTLVLMRNMGSAFETILELD
                     ETLIMVGRPKPWNPVYLLTRDDNCCDPGFYSLNYYRPGFDQTNKLTYVFDKSIIVQKD
                     VELISENLPPFRFELKSDLSLHVNADPASSEIGKYMTNASGFAVASKVGTDGRTWWLV
                     LMPVETHSLKAGWMPSNDLKRKF"
     gene            2403..2630
                     /locus_tag="VGA21_14930"
     CDS             2403..2630
                     /locus_tag="VGA21_14930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651852.1"
                     /translation="MAKITRRNSGSKAGGQGSYNPVVSFQSRVTRLEYVKGSTESGKL
                     ADLVALEKDPTSGDLFSIMEIKVERTMIDGN"
     gene            2655..4121
                     /locus_tag="VGA21_14935"
     CDS             2655..4121
                     /locus_tag="VGA21_14935"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018623226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018478 - malonate-semialdehyde
                     dehydrogenase (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEX9651853.1"
                     /translation="METLLKFKEARNFINGTFIDSDALTMEVISPLDGRTISTIPLSG
                     ATDLDNAVEYAMAAYNDWSSTPIKERVQILFIYRNLLNKYRDELTELVHIENGKIFSE
                     AEAEVDKAIELTEFACSLPQIVPGEFLEVSKGVECRIERIPLGVVASITPFNFPNMVP
                     NWTIPIVIGLGNCMILKPSEKVPLTAARLALILKEAGLPDGVFNVINGSQEIVEAICD
                     HPSIKAVSFVGSTRVAKIVYKRATSNLKRCLALGGAKNHLIVLPDAQTEMTASNIAAS
                     MTGCAGQRCMAASAMVAVGEIDHIINKLVEEAKKIIPGKNLGAVISKEAKERIENYVT
                     QAEKEGAKVLLDGRNVKVSGYENGFYVGPTILDFVIPDMAIAKEEVFGPVLAIMRTDS
                     LEKAMEIEKSSIYGNAASVFTQNGRAAKFVADNASAGMIGVNIGVPVPREPFSFGGMN
                     ESKFGSSDITGKSSIEFWTQIRKTTTKWSPESRINWMS"
     gene            4123..5466
                     /locus_tag="VGA21_14940"
     CDS             4123..5466
                     /locus_tag="VGA21_14940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020603430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX9651854.1"
                     /translation="MQTTTLHQTLENSLNYTLFSWSKQAGQNPISVKRTKGVYLYDTD
                     GKRYLDFSSQLMNVNIGHGDPRVKEAILKQMEEVNYVYPGAITEIRGKLGEKIAQIAP
                     EGMSKTFFTLGGADAIDNAIKLARHYTGRSKIVSLYRSYHGSTYGAMSAGGDPRKHGV
                     DNQQVPNSIHVENPYFYRCPWHSKTPEECSERAAAQMERVIQFENPESVAAILMEGES
                     GSSGCIKYPPGYWDRVRKIANKYGILTISDEVMSGFGRTGKWFAIENHNVIPDIICMA
                     KGLTSGYLPLGGILVSDTISRHYENETLPLGLTYSAHPLSCAAALEVMNIYETDKLFE
                     NAFQMGKYIEAAITQLMDKHPSVGDFRNTGLLGCIELVKNKKTKEPITPWNAKPNQME
                     ATNKILSVIRESGMFTFVRWNYIFVCPPLIISRQEVDEGMNIISRALSVADEYCD"
     gene            complement(5470..6390)
                     /locus_tag="VGA21_14945"
     CDS             complement(5470..6390)
                     /locus_tag="VGA21_14945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651855.1"
                     /translation="MRRFTWLFTSLLILGGCNTLETIDEISNFFVDPDVEPIEGTLVR
                     SLPLAFAGQMSQKAVIGTEPDFVTIVSPCNAFPCTSVIKFKVGETGFPWFALPSNTTV
                     TVAGLWTSPQQGLLSVLFTINTPGSLKLIEVRTFPIQENFDGNLIIATGRIDINGGSG
                     PLLSLEVTEEEFNLEFDRFLTFTSLDSLIEVTQEVWMIEIMDNGTEELSDDIYSLTGA
                     GQFATVAQEKVMALQIAALFVEVSANCLTNPINGDILIQMGGTSGDTPQVGQAQLEFT
                     GNCNATVKVNIATGSYVGAFGKNIGFNILD"
     gene            complement(6458..7195)
                     /locus_tag="VGA21_14950"
     CDS             complement(6458..7195)
                     /locus_tag="VGA21_14950"
                     /inference="COORDINATES: protein motif:HMM:NF014724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEX9651856.1"
                     /translation="MKVTRIHKIIVVISAVLLSPIVVFWAGRKVFTYVFIATMSGCSV
                     FYPGMYETFENGLENQPYDAIIVPGVPFEGESWSSIMKIRVHWSYYLYQKGYTKNIIY
                     SGGAVYTPYVEAKVMSLYGKALGIPDQRIFTEERAEHSTENVYYSYKMAQDLGFKNIA
                     LATDIFQLNNLRSFIQKYNLDVKLLPIVFDTLATLNRYEPVIEASIALTDTLTFIPLP
                     ERESFFKRLQGTLGNNIEFEEEDLVDE"
     gene            7363..>9170
                     /locus_tag="VGA21_14955"
     CDS             7363..>9170
                     /locus_tag="VGA21_14955"
                     /inference="COORDINATES: protein motif:HMM:NF039501.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5686 and carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEX9651857.1"
                     /translation="MSRVFRILFIYGLWLAAHSALAVSGPGGVQGTITTEDGETLPYS
                     TIYVRNIQTGTSSNAEGFYRISLDPGKYDLVFQYMGYETSVQFVEVGQTFLELNITLK
                     PQIIVLNTVEITARDEDPSYTVIRKAIAKAKYHTQIIDTMSARVYIKGSGRLISAPFF
                     ARNQLKKEGIDSTSAFIQESVSIIKYKRPNTIEEQVISIRTIGDAQNQSPNAYINGSF
                     YEPEIAGAISPLSPRAFAYYIFEHQGTFFDRGVMVNKIKVIPRSKGDDIFWGFIYIVE
                     DSWAIHSLDLKATKLGINFHLRQICDQIEPNIWLPVNHNVEGDGKILGFDFEFQYLVS
                     VSDFYIELNPELDFEFEVIDEKVNPELAKELKPKLDIKTAQLEEKLANGNEITRKELR
                     KVLKEYEQMELEEFEMPDILQDYKMIIDSTAYDFNPSYWAEIRPIPLSKYEVRGYEKM
                     DSMAVAEKEEQLKDSLKAERKKNFHVEDLLLGNSYRITDQSRFRIKPFLENLGFNSVE
                     GYNLTYGLRYDGNYKGRKRLSFGPDVRYSFARNKVTGRGVMEYRFGERLTPGRIYGEG
                     GKYVVQYNHAEPIHPIVNSFMSLLFSKNYMKIYERD"
BASE COUNT         2841 a         1777 c         1954 g         2598 t
ORIGIN      
        1 cactcttgat ctgcggattt acgattctca ctacgagcca agctcactcc caggctatgg
       61 taacaagagg ccaggaagta caattaatct ggtttggtgg ggtaactgca atctcttttc
      121 ataatgtcca ggtcgaaact gcaaatggaa gtcttcagtt cacggtgagt tttcagttgc
      181 cagacggcgc tccgggagta aacaagaaag catacacact tcccttatat tggatttcgg
      241 aatttggtat tcatgttggc agcggcatag tcacacctaa tggcaaagta attgcccgtt
      301 ttcatgtaaa aacagaaaac aggacttagc gtcctggaaa ttattgttat tcgcaattgc
      361 caccttcgta ataaggtcaa acaatggttt tttggctatt cctttaggca tgtaaataag
      421 tatttaatat attgacggta ataaaacctt caactatgaa aaatctaaaa gttttgtttc
      481 tctgcgcgct ttttgttttt ccttttcggg cattggcgca ggaagatttg atagagtatc
      541 tgccagccgg tgaagctgat gctaaatttc tgatggacca atatttatct atcatggcgg
      601 cggttgttga gtctgggctt aatagcggtt ggtatactac cggtgatcct cattataaat
      661 ttgggtttga gataaatgcc gctcttaata caatttttat tccctccagg gaaaatttct
      721 tcacagttac ttcaagtgat ttgggtaatt cttcattagt aaaaagagaa actgatggct
      781 cctataccat attgactacc ggggaagtgc cgacaggata tggacccgaa gatgtaactc
      841 ccatttttca aataacaaat ccagcagatc ctaatgcagg tgcgattttc aaaggaccga
      901 gcggcaatga tttaagcgat gagttcctaa taaatgccgt aatagtacca tctcttcagc
      961 ttggaattgg cattccttat aacatggata tcaaaatcag gtatactccc cagtatgact
     1021 acggtgatgt gaagtttgga aactggggcg ttggattaat gcattccatc aaacagcata
     1081 tcccggcact caaagtatta ccatttggct ggtcattgtt tattgcatat tcaaggctta
     1141 acggggaaat agatttttca ggccaatgga caggaaatga tcagttgggt gaattccatt
     1201 ccaacacctg gacttttcag accattgcat caaaagactt ttcaatcttt accgtatacg
     1261 ggggtatagg ttataatatc gggaatactg aatttgatat taagggaacc tataatattg
     1321 ttgatgttaa tcccggcgac ggaatgaggg tacctcttaa cactcctatt gtggcggacc
     1381 ttacgtcaaa cccctaccac tttgcgtatg acagtcagag cttacgaatg acccttgggc
     1441 ttaggatgaa attaggtccg cttattttct ttggagatta cacattacag agaacgagtg
     1501 ttttgacgtt tggaatgggc ttcaccttca aagaggatga attctaattt ttaatttaga
     1561 attttctctt aaggtcgttg ctgggcatcc agcctgcctt aagggagtgc gtttcgacag
     1621 gcataaggac aagccaccag gttctgccat cagttccaac tttagaagcc acggcaaatc
     1681 cggaggcgtt agtcatatat tttccaattt cagagcttgc gggatcagca ttgacatgaa
     1741 ggcttaaatc actctttaac tcaaatcgaa atggaggtaa attttcactt atcagctcaa
     1801 catctttctg aacaattata gatttgtcaa atacataagt cagcttattg gtctggtcaa
     1861 atcctggccg gtaataattc agagaataaa agcccggatc gcagcaattg tcgtctcttg
     1921 ttagcaaata aacaggattc catggtttag gtctgccaac cataataaga gtttcatcca
     1981 actccagaat agtttcgaaa gcggaaccca tattacgcat taatactagt gtgttgcctc
     2041 cggaaattga gatcacatcc ggtatttgat tgccgtcaat atcaacaatc tttaccgttg
     2101 ctgcattcat ggtttgcagt ccggacttgc ttaagaattc agatttttcc tgatcattat
     2161 cataaatttc ccagtaactc caatcgaaag aactgtgatg gaggaaatca ctaatctgtg
     2221 caaatgtggc attgaacaaa ataagtaaaa atactgtaaa aaggcagggt ttagtattca
     2281 tctttggcat gtcagtaaaa catattaata tactaaaact atatatcggt acaaagaaat
     2341 cccatcagtc ggatggctaa aatatttcaa tatttcgtaa cttctggttc taaaccatct
     2401 acatggccaa aataacaagg cgaaattctg ggtcaaaagc cggtggccag ggatcttaca
     2461 acccagtggt atcatttcaa tccagggtaa cgcggttgga atacgtcaaa ggctccacag
     2521 aatctggaaa attagcggat ttagtagcac tggaaaaaga tccaaccagt ggagatctct
     2581 tttcaattat ggaaataaaa gttgaaagaa cgatgatcga tggaaattag gtgtatgaat
     2641 cttaacttga tttaatggaa actttactga agtttaaaga ggcaaggaat tttattaacg
     2701 gaacatttat agatagtgat gctctcacta tggaagtaat tagccccctt gatggtcgga
     2761 ccatttctac tataccttta tcgggagcta ctgatttgga taacgccgtc gaatacgcga
     2821 tggccgcgta taatgattgg agttcaactc ccataaagga aagagtacaa atacttttta
     2881 tctatagaaa tcttcttaat aaataccggg acgaactaac ggaattagtc catatagaaa
     2941 atggaaaaat attttctgaa gccgaagcag aagttgacaa agctattgag ctgacggaat
     3001 ttgcctgttc cctgcctcaa attgtgcccg gagaatttct tgaggtaagc aaaggtgtgg
     3061 agtgcaggat tgaaagaatt cctctcggag ttgttgcctc tattactccg tttaattttc
     3121 ctaatatggt tcccaactgg actattccca tagttattgg attgggaaac tgtatgattt
     3181 tgaaaccgtc cgagaaagtt cccctgactg ccgcgcgatt ggctttgata ttgaaggagg
     3241 ccggcttacc ggatggcgtt ttcaatgtta ttaatggttc gcaggaaatt gtggaagcta
     3301 tttgtgatca tccatcaatt aaggccgttt cttttgttgg ctcaacccgg gtagccaaaa
     3361 ttgtttataa gcgggcaacc tcaaatttga aacgctgtct tgcactcgga ggtgctaaaa
     3421 atcatctcat tgtacttcct gatgcacaaa cagagatgac ggcgagtaac attgcagcca
     3481 gtatgacagg atgtgcagga cagcgttgta tggcagcatc ggcaatggtg gcagttggtg
     3541 aaattgatca tatcatcaat aaacttgtgg aagaggcgaa gaaaattatt cctggtaaga
     3601 acctgggtgc agttatttcc aaagaagcaa aagaaagaat tgaaaattat gttacgcaag
     3661 cagaaaaaga aggagccaaa gttttgcttg atggacgtaa tgtgaaagtt tccggttatg
     3721 aaaatggttt ttatgtcggt cctaccattc ttgattttgt aataccggat atggcaatcg
     3781 caaaagagga ggttttcggt ccggtgctgg ctatcatgcg tacagattcg cttgagaaag
     3841 cgatggagat tgaaaaatca tccatatatg gaaacgcggc atccgtgttt acacaaaatg
     3901 gcagggcggc caaatttgtt gctgacaacg cgagtgccgg aatgattggt gtcaacatcg
     3961 gtgtgcctgt tccccgcgaa ccattttctt tcggaggaat gaatgaatcg aaatttggat
     4021 cttctgatat taccggaaaa agttcaatag aattctggac tcaaatcaga aagacgacaa
     4081 ctaagtggag tcctgaatcc cgaataaact ggatgagtta atatgcagac aactacctta
     4141 catcaaactc ttgaaaatag cctgaattat actttattct cctggtcaaa acaagcaggt
     4201 cagaatccaa tttctgtaaa gcgaacaaag ggagtgtatt tatacgatac tgatgggaaa
     4261 aggtatctgg atttttcctc tcaattaatg aatgtaaata tcggccacgg tgatccccgg
     4321 gttaaggaag ccatcttgaa acaaatggaa gaggttaact atgtttatcc cggagcaatt
     4381 acagaaatca ggggaaagtt gggtgagaaa attgcacaaa tcgcgcctga agggatgagc
     4441 aaaacattct tcacactggg aggtgccgat gccatcgaca atgcaatcaa gttggcccgc
     4501 cattataccg gccggtcaaa aattgtatcg ctttatcgat cttatcacgg ttcaacatat
     4561 ggggctatgt ctgcgggggg tgatccgcgc aagcacggag tcgacaacca gcaagtacct
     4621 aactctatcc atgttgaaaa tccctatttc taccggtgcc cgtggcatag taaaacccct
     4681 gaagaatgtt cggaacgcgc agctgcccaa atggaaagag taattcaatt tgaaaatccg
     4741 gaaagtgttg ccgctatttt aatggaaggt gaatcgggca gctccggctg cataaagtac
     4801 ccgcctggtt attgggacag agtcagaaaa attgcgaata aatatggaat cctgacaata
     4861 tccgacgaag taatgagcgg gtttggccga acaggtaaat ggtttgccat tgaaaatcac
     4921 aatgtgattc cggacatcat ttgcatggcc aaaggcctaa cttccgggta cctgccgttg
     4981 ggcggtatcc ttgttagcga taccatttca aggcattatg aaaatgaaac ccttccactt
     5041 gggctgacat acagcgctca cccgttaagt tgcgcggccg ctttggaagt aatgaatatc
     5101 tatgaaacag ataaactttt tgaaaatgct tttcaaatgg gtaaatatat tgaagccgcg
     5161 attactcaat taatggataa gcatccatcc gttggcgatt tcagaaatac cggattattg
     5221 ggctgtattg aattagtaaa aaataaaaaa accaaagaac cgattactcc ctggaatgcc
     5281 aagccaaatc agatggaagc aaccaacaaa atactatctg ttatccggga gtctggcatg
     5341 tttacatttg taagatggaa ttatatcttt gtttgtccgc cgctaataat ttccaggcaa
     5401 gaagtggatg aagggatgaa tataatttcc agggcattgt ccgtagcgga tgagtactgc
     5461 gattaaaact caatcaagaa tattaaagcc aatattttta ccgaatgctc ccacatagga
     5521 tccggtagct atattcactt taactgttgc attacaattg ccggtaaatt cgagttgtgc
     5581 ctgacctacc tggggagtgt caccggaagt tcctcccatt tgtatcagaa tatctccatt
     5641 gattggattt gtaaggcagt ttgctgaaac ttcaacaaac aaagcagcaa tctgcaatgc
     5701 cataactttt tcctgggcaa cagttgcaaa ttggcctgct ccggtgagcg aatatatatc
     5761 gtctgacaac tcttctgttc cattatccat aatttcaatc atccatacct cttgcgttac
     5821 ttctatcagg gagtccaggg aagtaaaggt caaaaaccga tcaaattcca aattaaattc
     5881 ttcttcggtt acttccaggg acagcaacgg accgctgccg ccattgatgt caattctgcc
     5941 ggtagcaata atcaggttcc catcaaaatt ctcctgtata ggaaatgttc ttacttcaat
     6001 gagtttgagt gatcctggcg tattgatggt aaaaaggact gaaagcaaac cttgttgtgg
     6061 cgaagtccat agcccggcta ctgttaccgt cgtattcgag ggtaatgcga accaggggaa
     6121 ccctgtttct ccaactttga atttaattac cgaagtgcat gggaatgcat tgcaaggact
     6181 aacgatcgtt acaaaatcag gttcagtacc tatgactgcc ttttgagaca tttgtccggc
     6241 aaaggctaat ggtagcgacc tgaccaaagt tccttcaata ggctccacat ccggatcaac
     6301 aaaaaagttt gaaatttcat cgatggtttc aagcgtgttg catccaccaa gaatcagtaa
     6361 ggatgtaaag agccaggtaa aacgtctcat tctttaaaga atattcaata aagatagaat
     6421 tttataaaaa caaaagggaa ttaatcggat tcaatactta ttcatcaacc aaatcttcct
     6481 cttcaaattc aatattatta cctaaagtac cctgcaaacg cttaaaaaac gattcccttt
     6541 caggaagggg aatgaaagtc aatgtatctg taagggctat tgaagcttca ataaccggct
     6601 catagcgatt cagggtagcc agtgtatcaa aaacaatcgg caacaatttc acatccaggt
     6661 tatacttttg tatgaaggat cttaaattat tcaattggaa aatatctgta gctaaagcaa
     6721 tgtttttgaa tcccaaatcc tgggccatct tgtaagagta atacacgttt tctgtgctgt
     6781 gttcagctcg ttcttcagta aaaatacgct gatcaggaat tcccaatgcc ttaccataaa
     6841 gtgacattac ctttgcttcc acataaggag tataaaccgc gcctccggaa taaattatgt
     6901 tctttgtata acctttttga tacagataat aagaccagtg aaccctgatt ttcataatgc
     6961 tgctccagga ttccccttca aatggaacgc caggaactat gatggcatca taaggctggt
     7021 tctccaatcc attttcaaag gtttcataca tacctggata aaaaacggaa cagccggaca
     7081 ttgttgcgat aaacacgtag gtaaatactt ttctgccagc ccagaaaaca acaataggag
     7141 ataacaaaac agcagaaatg acaacaatga tcttatgtat gcgggtaact ttcatttaaa
     7201 acattaataa aatatcctgt cagctaatag agcaatttgt aacattgcga cctttgagcg
     7261 gtcataataa tattctatgc aactgatata tgcttcctgc cgtcttgtta ctaaatttaa
     7321 aaatagaaca aatttgataa ttcacttgtt ttagcttcaa caatgagcag ggtttttaga
     7381 atcttattta tttatggttt atggttggct gcccattcgg ctttagccgt ttccggtccg
     7441 ggtggggttc agggaacaat tactaccgaa gatggtgaga cacttcctta ttccaccata
     7501 tacgtaagga atatccaaac cggcacctcc tccaacgccg aaggatttta caggataagt
     7561 ttggatcccg gaaagtatga ccttgttttc cagtatatgg ggtatgaaac atccgttcaa
     7621 tttgttgaag ttggacaaac ttttttagaa ttgaacataa cactaaaacc tcaaatcatt
     7681 gttctgaaca cagttgaaat cacggcaaga gatgaagatc cttcttatac ggtgatcaga
     7741 aaagccatag cgaaagccaa gtatcacacc caaattattg atacgatgag tgcacgagtt
     7801 tatattaaag gctccggccg gctaataagc gcgccattct ttgcgcgtaa ccaattaaag
     7861 aaagaaggaa ttgattcaac ttcggcgttc atccaggaat ctgtcagtat tataaagtac
     7921 aaaagaccaa ataccattga agaacaggta atttcaatcc gcacaattgg tgacgcgcaa
     7981 aaccaatccc cgaatgcata cataaatgga agtttttacg aacctgaaat tgccggcgcc
     8041 atatcccctt tgtcgccaag ggcattcgct tactatattt ttgagcacca gggaaccttc
     8101 ttcgacaggg gagttatggt gaacaagata aaggtgatac cgagatcaaa gggtgatgat
     8161 attttttggg ggttcatcta cattgtcgaa gattcctggg caattcatag cctggacctt
     8221 aaagctacta aattgggaat caactttcat ctcagacaaa tttgtgatca aatcgaacca
     8281 aacatttggc ttccggtaaa ccacaatgtc gaaggtgatg gtaaaatcct gggatttgat
     8341 tttgaatttc agtacctggt gagtgtgagt gatttttaca ttgaattaaa cccggagctg
     8401 gattttgaat ttgaagttat tgatgagaag gttaatccgg aacttgccaa agaattgaag
     8461 ccaaaactgg acatcaaaac agctcagctc gaggaaaaac ttgccaatgg taatgaaata
     8521 acgaggaaag aactcaggaa agttctgaaa gaatatgagc agatggaact tgaggaattt
     8581 gaaatgcctg atattcttca ggattacaaa atgattattg actccacagc gtacgatttc
     8641 aatccctctt actgggcgga aatacgacca atcccactgt ccaaatatga agttcgtgga
     8701 tatgagaaaa tggactctat ggctgtagct gaaaaggagg agcaacttaa agacagttta
     8761 aaagcggagc gtaagaagaa ctttcatgtt gaggatttat tgcttggcaa ttcgtatcgt
     8821 attactgacc aaagcagatt caggattaaa ccttttcttg aaaatttggg ctttaattct
     8881 gttgaaggtt acaatctaac ctatggattg cggtacgatg gaaattacaa gggccggaaa
     8941 aggctgtctt tcggacccga tgtccggtac tcctttgccc ggaacaaagt aaccggtcgc
     9001 ggtgtaatgg aatacagatt tggagaacga ttaaccccag gaagaattta tggagaagga
     9061 ggaaaatacg tagtgcagta taaccatgcg gaacctatac atcccattgt aaatagcttt
     9121 atgtccctat tattcagcaa gaattacatg aaaatttatg agcgggatta
//
LOCUS       DASWHX010000237         2480 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_297752, whole genome shotgun sequence.
ACCESSION   DASWHX010000237 DASWHX010000000
VERSION     DASWHX010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 2480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2480)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2480
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297752"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..624
                     /gene="era"
                     /locus_tag="VGA21_14960"
     CDS             <1..624
                     /gene="era"
                     /locus_tag="VGA21_14960"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013453834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEX9651858.1"
                     /translation="ADIIIYIVELGQKFDPDDEVIRRIKKSEKKLILVINKVDLDKDK
                     LLESMMAEWQVQLQPDELIPVSALKSFNINSLLDAIINLLPEHPAYFPKDEITDKPER
                     FFAAEIVREKIFLNYKQEVPYSSEVVITDFKEDEKIIRIRAEIYVERDSQKGILIGKG
                     GRSLKKVGIESRADMEKFFGKQIHIETFVKVEPDWRKKITSLKKFGY"
     gene            complement(629..2104)
                     /locus_tag="VGA21_14965"
     CDS             complement(629..2104)
                     /locus_tag="VGA21_14965"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX9651859.1"
                     /translation="MKVYIQAFLIFFLLIFISCGQAPQAGSDPVTKSLEYQSWETTQQ
                     PEGTAAYLGLGSSAKVLCTAVFASNRDPEEAFTNSGKIILAEEFARDAAYEINFETKQ
                     VFIFLGDSVRRSATYVGDQGCIIDSPEGLKFTPVDVISSLLPADSLDWPMGDKLTGSL
                     NTYSSELLDSVNAAAFNPKALTAAFLVIHKGNIILEQYDQGVDRNTQLESWSMGKSLT
                     ATLIGRLIQMGKLSLEQKAPVKEWQREGDPRGEITIRNLLNMSGGLRFPAHRDPEVKV
                     PLALLAHMYIYTEAINVFDFAVNRPLEFVPGSTGLYRNCDPLTLGYIFRTIVESEGRN
                     YFTWPQEELFDKIGIRRQLLETDPYGNFIMTGFDYGTARNWGRLGLLYLQDGMWQGER
                     LLPEGFVGFVSTPAPGWSEPIYGGLFWLNGTGALSVPRDAFYMAGGGGQKTIIVPSLD
                     LVVVRMGHSDGSTYADDSLNEALEKIVRLVSTAKESGTTDN"
BASE COUNT          782 a          513 c          486 g          699 t
ORIGIN      
        1 gcggacatta ttatttacat tgtggagttg ggacaaaaat ttgatcctga tgatgaagtc
       61 atccggcgga ttaaaaaatc cgaaaaaaaa ttgatactgg tgatcaataa agttgacctt
      121 gacaaagaca agcttcttga atccatgatg gcggaatggc aggttcaact ccagccagat
      181 gaattaattc cagtgtcagc actgaagagt ttcaatatta attccctttt agatgcaata
      241 atcaatttgc ttccggaaca tccggcctat tttcccaaag atgaaattac agataagccc
      301 gagcgttttt ttgccgcgga gattgttcgt gaaaagattt tccttaatta taaacaagaa
      361 gtgccctata gcagtgaagt ggttattacg gattttaagg aagatgaaaa aattatcagg
      421 attcgggctg aaatatatgt ggaaagagat tctcagaaag gaattctgat tggtaaaggc
      481 ggcaggtcgc tcaaaaaagt aggaatagaa tcaagagcgg atatggaaaa atttttcgga
      541 aaacaaattc atattgaaac ttttgtgaaa gtggaacctg actggcgaaa gaaaattacc
      601 agcctgaaga agtttggata ttagtttctc aattgtcagt tgttcctgat tctttagccg
      661 tgcttaccag cctaacaatt ttttccaaag cctcattcag ggagtcatcg gcgtacgtgg
      721 aaccatcact gtggcccatt cttactacga ctaaatccag cgacggaacg ataatcgttt
      781 tttgtccgcc accaccggcc atataaaatg catcccgggg aacagacaat gcaccggtac
      841 cattcagcca aaacaaacca ccataaatag gctcgctcca acccggagca ggagttgaaa
      901 caaaaccaac aaaaccttca ggcaataatc tttctccctg ccacattcca tcttgcaaat
      961 aaagcaatcc cagacgcccc cagtttcttg cggtgccata atcaaacccg gtcataataa
     1021 aattgccata tggatctgtt tccaataact gtctgcgtat tcctattttg tcaaataatt
     1081 cttcctgcgg ccaggtaaaa taatttcttc cttcagattc tacaatggtt cgaaatatgt
     1141 atcccagcgt gagcggatcg caatttctgt acaaacctgt actgccagga acaaattcta
     1201 atggcctgtt aacggcaaaa tcgaaaacgt tgattgcttc ggtatatata tacatatgag
     1261 ccagcaatgc aagaggtact tttacctccg gatctctgtg agcaggaaat cgaagtccgc
     1321 cactcatatt taacaaatta cgaatcgtaa tttcacccct gggatcaccc tcccgttgcc
     1381 attcttttac aggtgctttt tgctccaggg atagtttccc catctggatc aatcggccaa
     1441 tcaaagttgc cgtcagactc ttacccatcg accagctttc taattgcgta ttcctatcaa
     1501 ctccttgatc gtattgttcc aaaataatat ttcccttatg gataacaaga aatgctgccg
     1561 tcaatgcttt tgggttaaaa gcggcagcat ttacagaatc aaggagctcg ctggaatatg
     1621 tattcaggct tccggttaat ttatcgccca tcggccaatc caatgaatcg gcaggaagaa
     1681 gagatgagat aacgtcaacg ggggtgaatt tcagaccttc aggagaatca attatacatc
     1741 cctgatcacc gacataagtt gccgatctgc gtacgctatc acccagaaaa ataaaaacct
     1801 gcttagtttc aaagtttatt tcataagccg cgtctcttgc aaattcctca gcaagaataa
     1861 tcttgccact attcgtgaat gcttcttcag ggtcgcgatt ggatgcaaat actgccgtgc
     1921 ataagacttt agctgatgaa cccagaccca ggtaggccgc tgtgccttca ggttgctgcg
     1981 tagtttccca ggattgatat tccagggatt ttgtgacagg atctgatcct gcctgtggag
     2041 cctggccgca tgaaataaat attagcagaa agaaaatcaa aaaggcttga atgtacactt
     2101 tcatggcgtg ggaattgaaa ttctaaatgg ctccaaagta atagagaatt ttccagaaaa
     2161 ggaaaagcaa ttattttgga atctcattat tacttattct ctcttcaaat tatatctggt
     2221 ggataaaccg ggatatttag ttcaattgta aaggtgtaat cacaagagag atatttgaat
     2281 agtacaacag aatataaatc gtattatata aatatttaat tttcgaatct atttcaataa
     2341 atagaagtta aggcgatccc gaaaagcctg aaacagagta gggaaaatta aaccactatt
     2401 ttcaaatgaa ttttaatcga acaaattacg tctcttttat gcttgcagga ctaataactg
     2461 catcatgtca aattcgggat
//
LOCUS       DASWHX010000238         6199 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_1409391, whole genome shotgun sequence.
ACCESSION   DASWHX010000238 DASWHX010000000
VERSION     DASWHX010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 6199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6199
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1409391"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..351
                     /locus_tag="VGA21_14970"
     CDS             <1..351
                     /locus_tag="VGA21_14970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008630501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX9651860.1"
                     /translation="PNLCTIPESFSLQMPSRYYLKCLTDSKFDSIHFLELQELFEASQ
                     ELERLFRKMTEAILAGVINRHIELQTSGIEERFRSFCQRSPHLLQLVPHKYIASYLNI
                     DSTNFSKLFNTVKL"
     gene            412..1272
                     /locus_tag="VGA21_14975"
     CDS             412..1272
                     /locus_tag="VGA21_14975"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX9651861.1"
                     /translation="MNLLWLDRKEYPFKSRNAEINGQQMHYIDEGDGPVIVFVHGTPS
                     WSFDFRNVIKALSKSHRCIAVDHIGFGLSAKPANYPYSTQQHAENFKVFIKNLRLSTF
                     TLVVHDFGGPIGLHYALKFPEEVRKLVIMNSWLWSNTGDPDFIKFSKILKNPLLPFLY
                     LYMNFSARFLLPNSFGSKKLSKSILKHYTKPFASKNERYGTLGFARSLLSAQGWYEEL
                     WSLRNKISSKPALFIWGMQDKFITPKYLEKFESEFTNSITIRLENVGHFPQEEEPINV
                     SLAIKNFHDS"
     gene            1481..4495
                     /locus_tag="VGA21_14980"
     CDS             1481..4495
                     /locus_tag="VGA21_14980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017731397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX9651862.1"
                     /translation="MKQPFTLIGAILLMALSVQAQERIVSGKVTSVEDGTPIPGVNIV
                     LKSTMNGTTTDVDGAYKISVPSEGGTLIFSFIGFETQEINVGASTVIDVSMVLDVTEL
                     SEIVITGYGERQKRTYVGSISTLNSASIENRPVNTIDQVLQGQVPGLSLVASSGTPGS
                     VQEIRIRGISSLTASNDPLFVIDGIPVITGSTERGSTGSLDALASINANDVESISVLK
                     DATATALYGARGSNGVIIITTKKGKSGKPVISFNAQYGSVARATDAPEMLNAAQWDEL
                     YYEGRVNRGDAPDIATAKAMFNSGWDGVTSTKWSDEVLNKDARSQQYDISIRGGGEQT
                     NYYGSIAYFNQDGVNKGINLERYSGKINLSTYITDKVSFSNSLTISRTEQNGQLEGSA
                     YFGSPDAAYMFLRPTDAPYNPDGTINITNISGVIWNPLWFPEHNIREIIQGRVLGNSG
                     LIAEILPGLEFSSTLGIDYFTTEEINYGNRYYGDGASGNGTSYMYNSRSSNWVWKNQL
                     SYNFELGFSSKFGINLAYEAQKNNYKTIATGGFGFAADGLYYPNSAGTSDFSSGFVND
                     WSINSALVVLNYSFKDKIFFDGSFRREGNSRFHPDHRWGTFYAIGGSYVISEEGFLSG
                     SNILTFAKIRASYGKTGNAGIGLNSYQSFLGFSGDYNGLPAVSPSSVGNAELTWENSN
                     QFSVALQTSFLDRINLEVEYYNRLVYDMLFDVPLSRTTGFTSQTQNLGEMVNKGIELQ
                     ADADIIRSGSFTWNLGLNFTTVDNEVTKLPRDANGMEIGIETGTYKITEGQPAYAYYM
                     PTWAGVDPATGAPLWYTDETETATTSTLSLAGDAFQGMAPHATFFGALNTRVEFKGIY
                     LTASLYYSTGNGVYDTWAFYTQSDGSQTFTIANGYARQYDRWQQPGDISPNPINIYRN
                     TNQSNGLSSRRLYDGTYLRLRNVALGYNIPGNLVSKAKLSSARIYLMGNNFWTWKKDK
                     QFEWDPEQGSNATINLVPSVLKTFTVGINITY"
     gene            4514..5896
                     /locus_tag="VGA21_14985"
     CDS             4514..5896
                     /locus_tag="VGA21_14985"
                     /inference="COORDINATES: protein motif:HMM:NF025680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HEX9651863.1"
                     /translation="MKSTNNLFKYTATTIVAAALVLSSCDSEFLEPTVSTAKDLETNV
                     NNVDDLNNIVLGAYERLSQTGFLGRDIIAHGSVRSDDGFSNGNSGRFVASAQFEYQST
                     SGDPNAMWNDGYEVIANTNIVINAVTEDDGTQEADHIKGEAYAIRALAHMQLLLFFGQ
                     QNTGGTLGVPYITEYLGDLAPARLSVTEVWTKIGEDLNMASSLMSESLNDGPIRLTTY
                     GVAALKTRYFLFIQDYANVISNAQYVINSGLFQFANASNLAASYVGSSPATSIFELAF
                     TLSDNLGINGLYQIMNYTGYGDVLPLPGLYNLYDAGDARRNLIEYDATLDTYRMVGKY
                     PTSTYDDNVRVIRYAEVILNYAEALFMSGQTGPALTQLNSVPAAVGAQQWASVTLGNI
                     LDERRKELAMEGFRFHDLMRHGMDILKTDPSRQTFTGNSVPFGDPRLALPIPEAEINA
                     NSNIVQNTGY"
     gene            complement(5990..>6199)
                     /locus_tag="VGA21_14990"
     CDS             complement(5990..>6199)
                     /locus_tag="VGA21_14990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651864.1"
                     /translation="WKSIAGDKALINWQVPMGIAAMIVGCITVYSALFSTGYWIYGKV
                     LPAAILTLISIVGVLVLLRLWKWLN"
BASE COUNT         1904 a         1172 c         1282 g         1841 t
ORIGIN      
        1 cctaacttgt gcactattcc tgagtctttt tcactgcaga tgccttcccg ttactattta
       61 aaatgtttaa ccgatagcaa gtttgattct atccattttc tggaattaca ggaattattt
      121 gaagcctcac aagaattgga aagattgttc agaaagatga ctgaagcgat tttagcaggt
      181 gttattaaca ggcatattga attacaaaca tcgggtattg aagaacgttt tagatccttt
      241 tgtcagcgaa gtccgcatct gcttcaatta gttccacaca aatacatcgc ctcgtacctg
      301 aacattgatt caacaaattt cagcaaactt tttaatacag tgaaactata agttggtaaa
      361 gaccaacttt attgcagatt caattttctt cctttgaaga aaaaaggaat tatgaatctt
      421 ttatggcttg acagaaagga atatcctttc aaatccagga acgcggaaat caatgggcaa
      481 caaatgcatt acattgatga gggtgacggc ccggttattg tttttgtaca tggaacacct
      541 tcctggagtt ttgatttcag aaatgtaatt aaggcattat caaaatccca cagatgcatt
      601 gccgttgatc atattgggtt cggactatcc gctaaacctg caaattatcc ttattccacg
      661 caacaacatg ccgaaaattt taaggtattt atcaaaaatc tgagattaag tacttttacg
      721 ctagtagttc atgatttcgg tggacccatc ggtttacact atgcgttgaa gtttccggaa
      781 gaagtcagga agctggtaat tatgaacagc tggctgtgga gcaataccgg tgatccggat
      841 tttattaaat tcagtaagat tctgaagaac cctttacttc cttttctcta tttgtatatg
      901 aacttttctg cgaggttcct gttgcctaac tcctttggaa gtaaaaaatt aagcaaatcc
      961 attctaaaac attacaccaa accctttgca tcaaagaatg aaagatatgg gacattgggg
     1021 ttcgcacggt ctcttctaag cgctcagggt tggtatgagg agttatggag tcttagaaat
     1081 aagatctcaa gtaagcctgc actttttatt tggggaatgc aggacaaatt tattacccca
     1141 aaatatttgg aaaaatttga atcggagttt actaattcaa taacgattag gttggaaaat
     1201 gttggtcatt ttccccagga agaggagcca ataaacgttt cattggccat taagaatttt
     1261 catgattcct gatatttgtt tttttaatca actaatatga acaatataaa tattagaaga
     1321 atcaattggc atatatatgc attaccatcc aattttgttt taccgggatt catgtctttt
     1381 tcattcgttt agaaattaaa tttcacgttg taaaaaataa ttctaaatta gagcgtctaa
     1441 cttatattat taagtgtttt ttaactaaac caacaattca atgaaacaac catttactct
     1501 aattggagct attctgctta tggccctttc agttcaggcc caggagcgaa tagtctcagg
     1561 taaggttaca tcggtggagg atggcactcc aattcccggt gtaaacattg tgcttaaaag
     1621 tacaatgaat ggcacaacta cagatgtaga cggtgcctat aaaatctctg tgccttctga
     1681 gggaggaacg cttattttct catttatcgg atttgaaaca caagagatta atgtaggagc
     1741 ttcaactgtt attgatgttt ccatggttct agatgttaca gaattatcag aaattgtaat
     1801 aaccggttac ggtgaaaggc aaaagaggac ttacgtagga tctatttcca ctcttaacag
     1861 cgcttctatt gaaaaccgtc ctgtcaacac aattgatcag gtgttgcaag gccaggttcc
     1921 gggactaagt cttgttgcct catctggcac gccagggtca gttcaggaaa ttagaatccg
     1981 aggcatcagt tcgctaacag ccagcaatga tccattattt gtaattgatg gtattcctgt
     2041 tataacaggc tccaccgaaa gaggaagtac cggatccctg gatgcactgg catcaattaa
     2101 tgctaacgat gttgaaagca tctcggtact gaaagatgcc acagccaccg ccctctatgg
     2161 tgcaagagga tctaatggtg taataatcat tactacaaaa aaaggaaaga gtggaaaacc
     2221 ggtaatttcc ttcaatgccc aatatggatc ggttgcccgt gccacagatg cgcctgaaat
     2281 gcttaatgcg gctcagtggg atgaattgta ttatgaaggc agagtaaata gaggtgatgc
     2341 tcctgatatt gcgacagcaa aagcaatgtt taatagtgga tgggacggag ttactagtac
     2401 aaaatggtca gatgaggtac tcaataagga tgcaagaagc cagcagtatg atattagtat
     2461 tcgcggtgga ggagagcaaa ctaactatta cggctccatt gcttacttca atcaggatgg
     2521 tgtaaacaag ggaatcaatt tagaaaggta tagtgggaaa ataaatctta gcacctatat
     2581 cactgataaa gtgagtttct ccaattcatt aacaatatca agaacagagc agaatggcca
     2641 attggaaggt agcgcctatt ttggcagccc tgatgctgca tatatgttcc tgagacccac
     2701 cgatgctccg tataatcctg atggaacaat taacataacc aacatctccg gtgttatctg
     2761 gaatccgcta tggttccccg aacacaatat tcgtgaaatt attcaaggaa gagttcttgg
     2821 aaactctggt ttgattgcag agattcttcc cggccttgaa ttcagttcga ctctaggcat
     2881 agattatttc acaacggaag agataaacta cggcaacagg tactatggtg atggtgcatc
     2941 tggtaacgga acttcctaca tgtacaacag cagaagctct aattgggttt ggaaaaacca
     3001 gttaagttat aattttgaat tgggtttcag cagcaaattt ggaattaacc tcgcttatga
     3061 agctcagaag aacaactata agactatcgc aaccggtgga tttgggtttg cagctgacgg
     3121 actttattat ccaaattccg cgggaacttc cgacttttca tcaggctttg ttaatgactg
     3181 gtcaatcaat tctgcattgg tcgttctaaa ttattcgttc aaggataaaa tattttttga
     3241 tggttctttc cgcagggaag gtaattcaag atttcatcct gaccatagat ggggaacatt
     3301 ttatgcaatt ggcggttctt atgtgatttc tgaagaggga ttcctttcag gatcaaatat
     3361 tttgacattt gctaaaattc gagcttctta tggcaaaacc ggaaatgcag gaatcggact
     3421 taacagttat cagtccttcc ttggatttag tggagattac aatgggctgc ctgcagtctc
     3481 tccaagctca gtaggtaatg ctgaattaac ctgggaaaac agcaatcaat tttcagtcgc
     3541 tttacaaact tcattccttg atagaattaa cctggaagtt gaatattaca acagacttgt
     3601 gtacgacatg cttttcgatg ttccgctttc acgaacaacc ggctttacaa gtcagacaca
     3661 gaatttagga gaaatggtaa acaaaggaat cgaacttcag gctgatgcgg atattatccg
     3721 gagtggttct tttacctgga atcttgggtt aaattttaca acggttgata acgaagtaac
     3781 taagctgccg agagatgcca atggaatgga aattggaatt gaaacaggta cctataaaat
     3841 aacagaaggg cagccagctt atgcatacta tatgccaacc tgggctggtg tagatcctgc
     3901 aacaggggct cccttgtggt acactgatga aaccgaaact gcgacgacct ccactttaag
     3961 tttggcaggt gatgcatttc aaggaatggc tcctcatgct acattctttg gtgcattgaa
     4021 taccagagtg gaatttaaag gaatatatct tacagcctct ttgtattatt caacaggaaa
     4081 tggagtgtat gacacatggg ccttttatac tcagtcagat ggttctcaaa cgttcaccat
     4141 tgccaatgga tatgccagac aatatgacag atggcagcag cctggagaca tttctcctaa
     4201 tcctattaat atttaccgga atacaaatca gagtaatgga ttatcctctc gcagattgta
     4261 tgatggtacc tatctgaggc ttagaaacgt agctctggga tacaatatcc caggaaactt
     4321 ggtttcaaaa gcgaagctaa gcagcgccag aatttacctt atgggtaata atttctggac
     4381 gtggaaaaag gataagcagt ttgaatggga tccggaacaa ggctctaatg caactattaa
     4441 tcttgtccca tcggtcctca aaacatttac tgtaggaata aatattacgt attaatttta
     4501 aaatttgatg aacatgaaaa gtactaataa tttatttaaa tatactgcca caacaattgt
     4561 agcagctgca ctcgttttgt catcatgcga ttctgaattt ctggaaccta cagtttctac
     4621 ggcgaaagat ctggaaacta atgtaaataa cgttgatgac cttaacaata tagttttagg
     4681 tgcttatgag cgattaagtc aaaccggctt tttgggaaga gatattattg ctcatggatc
     4741 tgttaggtct gatgatggat tcagcaacgg taatagtggc cgttttgtag cttccgcaca
     4801 gtttgaatat cagtcaacat ccggagatcc taatgcgatg tggaatgatg gctatgaggt
     4861 aattgccaac actaatattg ttataaatgc cgtcactgaa gatgatggta cccaagaggc
     4921 agaccatatc aaaggtgaag cttacgcgat tcgggcactt gctcatatgc agttgctcct
     4981 tttcttcggt caacaaaaca cgggtggtac gctgggagtt ccatacatca ctgagtatct
     5041 tggagatctt gctccggcac gactatccgt tactgaagtt tggaccaaaa ttggagagga
     5101 tctaaatatg gcttcaagtt tgatgtctga atcccttaat gatggtccca tacggcttac
     5161 tacctatgga gttgctgcgc ttaaaacaag gtattttctt ttcattcagg attatgccaa
     5221 cgtaataagc aatgcacagt atgtaatcaa ttcaggtttg tttcaatttg caaatgcttc
     5281 aaacctggct gcttcctatg taggttcaag tccggcaacc tccatatttg aactggcatt
     5341 taccctgtct gacaatcttg gaataaatgg cttataccag attatgaatt ataccggata
     5401 tggcgatgtc ctgccgcttc ctggtcttta caatctttat gatgctggag acgcaagaag
     5461 gaatttaatc gagtatgatg cgacactgga tacttataga atggtcggaa aataccccac
     5521 ttcaacatat gacgataacg tgagggtaat tcgttatgcc gaagtaatat taaattatgc
     5581 agaggctttg tttatgagcg gacaaactgg tccggcctta acccaattga atagtgttcc
     5641 cgcagcggtt ggagctcagc aatgggcaag cgttaccctg ggcaatattc tggatgaaag
     5701 gcgaaaagag ttagccatgg agggctttcg ctttcatgat ctaatgaggc acggaatgga
     5761 tattctgaaa acagatccca gtcgccaaac attcacagga aacagtgttc ctttcggcga
     5821 tcccagatta gctttgccaa tacccgaagc tgaaatcaat gctaattcta atatagtaca
     5881 aaacacaggg tattaaacca gataactttt ttttgtaaaa caaaggccac tcgctaaaag
     5941 gtggcttttg tttttttttg tagaataatt tttataaata caactcaaat caattcaacc
     6001 atttccataa ccttaataag accaaaacac caacaattga tattaatgtt aatatcgcag
     6061 ctggaagtac ttttccataa atccaatacc cggttgagaa taacgcgcta tacacggtaa
     6121 tacaaccaac aatcatggca gcaattccca ttggaacttg ccaattgatc aaagctttgt
     6181 ctcctgcaat tgatttcca
//
LOCUS       DASWHX010000239         4033 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_298806, whole genome shotgun sequence.
ACCESSION   DASWHX010000239 DASWHX010000000
VERSION     DASWHX010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 4033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4033
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_298806"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..300
                     /locus_tag="VGA21_14995"
     CDS             <1..300
                     /locus_tag="VGA21_14995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651865.1"
                     /translation="PDMPGEDYPDFEELQTEANDGHIIIGTYRNNSGSGHVVMIVPGE
                     AEKYSDVLIFKGYDSLIKFPKVLECGTDHRESKTSAFDNISIEKLKKMKWYRLVN"
     gene            279..1469
                     /locus_tag="VGA21_15000"
     CDS             279..1469
                     /locus_tag="VGA21_15000"
                     /inference="COORDINATES: protein motif:HMM:NF024704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YARHG domain-containing protein"
                     /protein_id="HEX9651866.1"
                     /translation="MVQISKLSTVIFFSFAMFISYTSISQEFESISFVGYENEEVLLL
                     CRQDGKNRPCSLNENEMQIELKDINSQLIGKNELTLVYFSLESQFNWFLEFPNVNPYP
                     FVIELSNIPRTFYLDFDYNATAFDLVRFQAYLASTSDIEVVNQFPPNNLIELDLTNGE
                     TREIPIKAMDPIIVQDYLFYADYHDVKNFDLVYDIYRVKIGDWENPQRIFIKNYRNGW
                     RVTPDGKYLIVEIISGGYSPTCILFNIETKKYSILETKFRKRPTFYSYLKNAICFYDH
                     SISSKEQEVERFHYIPIPESFPYTPDWAMEFGESFINHYWIEEAPEDSLRTLDKSQLR
                     LLRNSVFARRGWRFTDQALADFFSQFEWYRDQLARNQGNDKIQLTNTDKYRSNLILKI
                     ENEK"
     gene            1576..2352
                     /locus_tag="VGA21_15005"
     CDS             1576..2352
                     /locus_tag="VGA21_15005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651867.1"
                     /translation="MKKTFLLGIVILFNLTDCKEKEVPLVADFDAEITGTSPNATIVF
                     TSSSTGADTYEWTFGEGANITTSSVISPSISVEKAGLLTVTLTIHRGAEENSITQDID
                     VPGNSAILEFTDVEFALGAGDLDYGRFFSVATGQVYIDTEVNANNGSTISLAFESLGQ
                     TLHYFQSPSSSDYSIPNATQTKVINFPAGALMSIAQFEAMDDDEILADLTINDDGNGF
                     GNGNIPGIVLFQISDGRIGAIKTRSINADRLLVDIKVQKY"
     gene            2788..3030
                     /locus_tag="VGA21_15010"
     CDS             2788..3030
                     /locus_tag="VGA21_15010"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX9651868.1"
                     /translation="MPNTYTQIYIQIVFAVKAFGNQLQKLRLSKGMSQEDLADESGLA
                     YTTINKLENGHLNTGIATLFDLAKGLKVHPRELFDF"
     gene            complement(3325..3732)
                     /locus_tag="VGA21_15015"
     CDS             complement(3325..3732)
                     /locus_tag="VGA21_15015"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013455726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX9651869.1"
                     /translation="MENDSKAPEPVGPYPYSKRVGNLLFLSGIGPRKKGSKDIPGVTL
                     GKDGKVFSYDIEEQCRSVFQNVKYVLEESGSSWENLVDVTVFLTNMDDFAIYNKVYAE
                     YFPYNRPCRTTVAVSALPTPIAIELKCIATIGE"
     gene            complement(3735..>4033)
                     /locus_tag="VGA21_15020"
     CDS             complement(3735..>4033)
                     /locus_tag="VGA21_15020"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX9651870.1"
                     /translation="LGFYGFEIYGNDALLRSVVVNAEKRSKGVGTEIIREALLKAREL
                     GVSNLYLLTFTAKGFFEKLGFSVINRRLVPNSIASTEEFANFCPDTAVCMTIKV"
BASE COUNT         1284 a          821 c          770 g         1158 t
ORIGIN      
        1 ccggacatgc ccggcgaaga ctacccggat ttcgaggaat tacaaacaga agccaatgat
       61 ggtcatataa ttattggaac ttatcgaaac aattccggca gcggccatgt agtgatgatt
      121 gtgccggggg aggcggaaaa atattccgac gttttgattt tcaaaggcta tgacagtcta
      181 ataaagtttc cgaaagttct cgaatgtggt acagatcata gagaatcaaa aacaagtgcc
      241 tttgataata ttagtattga gaaactaaaa aaaatgaaat ggtacagatt agtaaattaa
      301 gcacagtgat ttttttcagt tttgccatgt tcatctctta cacttcaatt tcgcaagaat
      361 ttgaatctat tagttttgtt ggatatgaaa atgaagaggt gttgttgctc tgtcggcagg
      421 atgggaaaaa tagaccatgt tccttaaatg agaatgaaat gcagattgaa cttaaagaca
      481 ttaattctca actaattgga aaaaatgagt tgaccctggt ctattttagt ttagaatctc
      541 aatttaattg gtttttagaa ttccccaatg ttaaccctta ccctttcgta attgaactaa
      601 gtaatatccc gagaacattc tacctagatt tcgactataa tgcaactgca tttgatttgg
      661 taagatttca agcgtattta gcttcaacaa gtgacataga ggtcgtaaac caatttccac
      721 caaacaattt aatagagctc gatttaacaa acggggaaac cagggaaatt cctataaaag
      781 cgatggatcc aataattgtt caggattatt tgttctacgc agactatcac gatgtaaaaa
      841 attttgattt agtttatgac atctacaggg taaaaattgg cgactgggaa aaccctcaaa
      901 gaattttcat taaaaattat cgaaatggat ggcgggtgac acccgatggc aaatacttaa
      961 ttgttgaaat catttctgga ggctattccc ctacttgtat cctcttcaat atcgaaacaa
     1021 aaaaatactc aatccttgaa accaaattca gaaaaaggcc tactttttat tcttatttaa
     1081 aaaacgctat ttgcttttat gaccactcta tttcgtcaaa ggaacaagaa gtcgaaagat
     1141 tccactacat tcccatcccc gaatccttcc catacactcc cgactgggct atggaattcg
     1201 gcgagagttt catcaatcat tactggatcg aagaagctcc ggaagattcg ctgcgcaccc
     1261 tggataaatc ccagctaaga cttttgcgaa actcagtctt cgcccgaaga ggatggcgat
     1321 tcaccgatca ggctctggca gattttttca gccagttcga atggtatcgg gaccagcttg
     1381 caagaaacca ggggaatgat aaaattcaac ttactaacac agataaatat cgctctaacc
     1441 tgattttgaa aattgaaaat gaaaaataat agttaaccaa aaaaaacgaa ttactatgac
     1501 acactaaact aacattcggt ccgcgtagaa ttcctgagaa gaaaaatttt gttaaacatt
     1561 taattaatta ttcatatgaa aaaaacattt ctactcggga ttgtcatcct atttaactta
     1621 accgattgta aagaaaaaga agttccatta gtggcagact ttgatgctga aataacggga
     1681 acttcaccaa atgcaacgat tgtttttaca agctcctcaa ccggagctga cacatacgaa
     1741 tggacattcg gcgaaggcgc aaacatcaca acttcctcag taatatcacc gagcatctct
     1801 gttgaaaagg caggcctgct gaccgttact ctaaccattc acagaggggc tgaggaaaac
     1861 tccattacgc aggatattga tgtgcctgga aattctgcaa tactggagtt caccgatgtt
     1921 gaatttgctc ttggtgccgg tgatctggat tacggaagat tcttttcagt cgccacggga
     1981 caggtataca ttgatactga agtcaatgcc aacaatggct ctactattag tttggcattt
     2041 gaaagcctgg ggcaaaccct gcattacttt caaagtcctt catcctcaga ttacagcata
     2101 cctaacgcaa ctcaaaccaa agtaataaac tttcccgcgg gtgcgttgat gagtatagct
     2161 cagttcgaag caatggatga tgatgaaatc cttgctgatc tgacaatcaa tgatgatggc
     2221 aatggttttg gcaacggaaa cattcctgga atagtactgt ttcaaatttc cgatggccgt
     2281 attggggcaa taaaaacccg atcaataaat gcggatagat tgctggtgga tataaaggtt
     2341 cagaagtatt aaccaggaat gtggattggt ggcactaata gcttactagt attcttaagt
     2401 tctggtgcca ctattcttct aataacaact aaaacattat tataatgatt aagaaatata
     2461 aagcacaaat tcttgcagca tgtttgaaaa tgtcctttcc tccccctcag tctccgaagg
     2521 gactctaagg cattaaattt ctgttacggt ttttagtgat aatcctgcac taggtttaca
     2581 atgttaaaca ctcctttttc ttacccacgc agtaggcgga tattcatatc cgtgcctgat
     2641 taattggttt gatttctcaa tccaacatca tcattctacc tattcaccct tgcaaaaata
     2701 gctaaacccg ttaagtccca tagggacgat cagtttgtag aaaataaaac aatgcaataa
     2761 gtaagctccg taggagcgac ctgaaatatg ccaaacacct acacgcaaat ttatattcaa
     2821 attgtctttg cagtcaaagc atttggaaat cagcttcaaa agcttcgcct ttcaaaaggg
     2881 atgtcccagg aagatttggc ggatgagtcg ggtctggcgt ataccactat aaacaagctg
     2941 gagaacgggc atcttaacac cggcatcgcc acgctctttg atttagcaaa agggttaaag
     3001 gtacacccaa gagaattatt tgatttttga agatgatcac cgctaacctg tttattgcct
     3061 tcgcagggtc tgccgagcca ccgcatcgaa actgccaatt gaattttaat aaacatcaag
     3121 gtgtgccaca cttccacttt ttatttaggt gaaaattgat tgattttcca tctccgcaaa
     3181 gtgcggcacc cctaagaggc tctatattgg caattgaatt tttgtaaata tcaaggtgtg
     3241 cgacacttcc aactatggcc ctcgtttgta acgatgtgcc agggcaatta ttaatcccga
     3301 taaagaatct aaatgatacg ttatttattc gccaatggta gcaatacact tcaattcaat
     3361 ggcaatgggg gtaggcagcg cactaacagc aactgttgtg cggcacggtc tgttgtatgg
     3421 aaaatattcc gcataaacct tattataaat agcgaagtca tccatattgg taaggaaaac
     3481 cgtcacatca accagatttt cccaggaaga tccggattct tccaaaacgt attttacatt
     3541 ctgaaagaca gaacggcact gttcttctat atcatacgag aatactttcc catcttttcc
     3601 taaagtaaca ccagggatat ctttggaacc tttctttctg ggtccgattc cggagagaaa
     3661 taaaagatta ccaacccttt ttgaataagg atatgggcca accggctcgg gagctttaga
     3721 atcattttcc atgcttaaac ttttatagtc atacatacgg cggtgtccgg acagaaattc
     3781 gcgaattctt cggtggatgc aatggaattt ggaactaacc ttcggttaat cacagaaaat
     3841 ccaagctttt caaaaaaacc ttttgccgtg aatgtcagca aataaagatt ggatacacca
     3901 agttccctgg ccttaagaag tgcttcccgg ataatttcgg ttcctactcc ttttgatctc
     3961 ttctctgcat taactacaac gcttcgcaac agggcatcat ttccgtaaat ttcaaaaccg
     4021 tagaaaccca gcg
//
LOCUS       DASWHX010000240         2515 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_3266424, whole genome shotgun sequence.
ACCESSION   DASWHX010000240 DASWHX010000000
VERSION     DASWHX010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 2515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2515
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3266424"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..969)
                     /locus_tag="VGA21_15025"
     CDS             complement(<1..969)
                     /locus_tag="VGA21_15025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018621360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX9651871.1"
                     /translation="MRDYEMFSLPNGIRVIHKQVSNTKIVHCGFILDIGSRDELAHQQ
                     GIAHFWEHMAFQGTRKRKAFHILNRLESVGGELNAYTTKEKICFYASVLDPFFENAIE
                     LLTDITFDSIFPPKQIDKERRVILEEMAMYLDTPEDALQDEFDKVVFGNHPLGNNILG
                     TEESVKSFSRKNFKEFISENLDTSRLIFSCVGNISLDHVKKFSRKHLVKIPSKYNNRK
                     RKPFGDYIPKNETKYRNLIQAQCAIGCPAYPIGNERRLPFFMLVNILGGPGMNSRLNL
                     ALREKHGFAYSIDANYNPFMDTGLFSILFGTDPKQLERSVHIVFKEL"
     gene            1079..2368
                     /locus_tag="VGA21_15030"
     CDS             1079..2368
                     /locus_tag="VGA21_15030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020528830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P N-terminal domain-containing
                     protein"
                     /protein_id="HEX9651872.1"
                     /translation="MRYQPISREFYKRNRANFISKIKPGSIAIFNSNDIMPTNADGQM
                     PFRQNNDLLYLSGIDQEQTMLVIFPDFPEKKNNEILFIQETSEEIAIWEGNKLTREQA
                     RQISGIDTICWLAEFPKLFNTMMSQAEYVYLNANDHIRANAVVETREVRFAKMCREKY
                     PAHKFERSAPIMGLLRGIKYEEEIDMLRTACNITEAGFRRILKMVKPGVMEYEIEAEL
                     LHEFVRSRSRGFAYEPIIASGFSSCILHYTQNNLECKDGDILLLDIGAEYGNYNADLT
                     RSIPVNGKFTTRQKEVYKSVLHVQREAFKLLVPGNNIQDYHKEIGRIMEAELIKLGLL
                     DKTDVKNQNPERPLYLKYFMHGTSHHLGLDVHDWVNWWRNLEEGMVLTVEPGIYIREE
                     SLGIRIENDVVIRENGVEDLMAGIPVEIAEIEDLMNS"
BASE COUNT          812 a          438 c          510 g          755 t
ORIGIN      
        1 caattcctta aatacaatat gtacagatct ttccaactgt ttggggtcgg ttccaaataa
       61 aattgaaaaa aggccggtat ccataaaagg attatagttt gcatcaatag agtatgcaaa
      121 tccatgcttt tccctgaggg cgagatttaa tcgtgaattc atgccgggtc cacccagaat
      181 attcaccaac ataaaaaaag gaagtcttct ttcgttacca atcgggtagg caggacaacc
      241 aatcgcgcat tgggcttgaa ttaaatttct gtacttggtt tcatttttgg gtatataatc
      301 accaaaaggc tttctcttgc ggttattgta cttcgaaggt atttttacca aatgttttct
      361 tgagaatttc ttaacatgat caagcgaaat atttcccaca caggaaaaaa ttaaccggct
      421 tgtatccagg ttttcactga taaattcctt gaaattcttc cttgaaaaag actttacact
      481 ttcttctgtt ccaagaatat tatttcccag cggatgattg ccaaaaacca ctttatcgaa
      541 ttcgtcttgc aaagcatctt ccggtgtatc cagatacatg gccatctctt ctaaaattac
      601 ccttctctct ttatcaattt gttttggtgg aaagatcgaa tcgaaagtta tatccgttaa
      661 taattcaatg gcattttcaa agaatggatc cagaacagac gcgtaaaagc aaattttttc
      721 tttagtcgta taggcattta attctccacc aacagattca agcctgttta atatgtgaaa
      781 agcttttcgc ttcctggtgc cctggaaagc catgtgctcc cagaagtgag caattccctg
      841 ttgatgagcc agttcatctc tacttccgat gtcaagaata aatccgcaat gaactatttt
      901 agtattggaa acctgcttgt ggattactct gatgccgttt ggcaatgaaa acatttcata
      961 atcacgcatg agagtataat tccttcaaaa ttaatataat tatggattat tgtagtgtta
     1021 acttggtaat agctttcaac tctattatta aatctaagcc cctaattctg ttttaaacat
     1081 gcgatatcaa ccaatatcac gggaatttta caaaaggaac cgagctaatt tcatttcaaa
     1141 aataaagcct ggttccattg caatctttaa ttccaacgat ataatgccta caaatgcaga
     1201 cgggcagatg ccttttcgcc aaaacaacga tttgctttac ttgtctggca ttgatcagga
     1261 acagacaatg ttggtaattt ttccggattt cccggaaaag aaaaacaatg agattctctt
     1321 cattcaagaa accagtgaag aaattgcaat ttgggaagga aataaattga ccagggaaca
     1381 agcgcgtcag ataagtggaa ttgatacaat ctgctggctt gcggaatttc caaaattatt
     1441 taataccatg atgtctcaag ccgaatatgt gtacctgaat gcaaacgatc atatccgggc
     1501 taacgctgtg gtcgaaacca gagaggtcag gtttgcaaag atgtgcagag aaaaatatcc
     1561 ggcgcataaa tttgagagat ctgctcctat tatgggtttg cttcgaggta ttaaatatga
     1621 agaggagata gatatgttga gaacagcatg taacataact gaggccggtt tcagacgaat
     1681 tctaaaaatg gtaaagcctg gtgtaatgga atacgaaatt gaagcggagt tattacatga
     1741 atttgtgaga agtcgatcca ggggattcgc gtatgaaccc attattgcca gcggttttag
     1801 ttcttgcatc cttcactata ctcaaaacaa tctggagtgc aaagatggcg acattctact
     1861 ccttgatata ggtgcggaat acggaaacta taatgcagat ttaaccagaa gcattcctgt
     1921 aaatggtaaa tttactacac gtcaaaaaga agtatataaa tccgtattac atgttcagcg
     1981 ggaagccttt aaattattgg ttccaggtaa taacatccag gattatcata aagaaattgg
     2041 caggatcatg gaggcggaac taataaagtt agggttgctt gataagactg atgtgaagaa
     2101 ccaaaatccg gaaaggccat tgtatttaaa atatttcatg catggaactt ctcatcattt
     2161 gggacttgat gttcatgatt gggtaaattg gtggaggaat ctggaagaag gaatggtttt
     2221 gacagttgaa ccgggaatct atatcagaga agaaagtctt ggtatcagaa ttgaaaatga
     2281 tgtggtgatt cgcgaaaatg gagtggaaga cctgatggct ggtattcctg ttgaaattgc
     2341 ggagattgaa gacctgatga attcataaaa tgaaaagttc tttaacaatg agagaagaac
     2401 taccctgggt tgcaggggaa ttgaaactgg aaaaattatg ttatactgat atctacaact
     2461 caaatgtttc aatttggaaa cttgattcgg ataatgaaac gctgggtttc tacgg
//
LOCUS       DASWHX010000241         4234 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_1805721, whole genome shotgun sequence.
ACCESSION   DASWHX010000241 DASWHX010000000
VERSION     DASWHX010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 4234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4234
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1805721"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1198
                     /locus_tag="VGA21_15035"
     CDS             <1..1198
                     /locus_tag="VGA21_15035"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HEX9651873.1"
                     /translation="DLTNTIFLLEKLGNPHFKFKAVHIAGTNGKGSSAHFIASILQSA
                     GYNAGLYTSPHLKSFTERIKINGKEVSEDFVGEFVESVKPLLSKISPSFFEMTVAMAF
                     DYFARKKINIAVVEVGLGGRLDSTNVLIPEVSLITNISYDHTDLLGNTLEQIAFEKAG
                     IIKNSRPVVIGKRQEEVAHVFSIKAKSESTRIYFASDEIKLKPLNGAYQIFRSGKIWI
                     ELMNVPIVTDYIIENLPGVLQTIILLRQRGFIITDEAIKEGIREMVFKTGLKGRWQKI
                     NDKPLAICDVAHNLDGILKVLKEIRKIKYNKLLMVWGCVKDKDIEPILAVLPKNASYY
                     FCQPDIPRAMDASLLREKAENVGLTGVAIPNVEKAFQRAVRDASEDDFIFVGGSTFVV
                     AELENL"
     gene            1200..1859
                     /gene="trmB"
                     /locus_tag="VGA21_15040"
     CDS             1200..1859
                     /gene="trmB"
                     /locus_tag="VGA21_15040"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009183484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEX9651874.1"
                     /translation="MARNKLLKFADNNQTVKVIQAGKPIYNSIKGNWRKLQFKNNNPI
                     ILEIACGRGEYTIGFARLFADRNFVGIDLKGSRLWAGAKIADKENLRNAAFLRTQMHM
                     LEDFFEENEVDEIWIIHPDPRPKKSDTRRRLTSPRYMEIYKRIIKQGGWIHLKTDSTS
                     LYEYSLEVLMDRLDIHGLLHTDNLDQSTYLSDHYGIKTRYELMFRVEGENIKYLKFQF
                     Q"
     gene            2287..>4234
                     /locus_tag="VGA21_15045"
     CDS             2287..>4234
                     /locus_tag="VGA21_15045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651875.1"
                     /translation="MKRRIVLLYFISSLITGVPSICQVTISSSPFNYLQNFDNLINTG
                     SAPWVDNSTLPGWYATENSVRASDGNSPQLTMFSFGTGTDRALGARQDNSTGEMAYGV
                     RLVNNSGKTFQSMFIDLDGEQWRKSADSIPQVIKFMFIISNSPINSIDISTINNTEFY
                     EFNFISPVNNSLPITALDGNLPANRYHLSASIKINFPPGYDILLKWLDPNDKTVDHGM
                     GIDNLNVVFSETILDYDVANISANFEDFFNSSIKYEIIDETNLSTFTNPDQLSIETWG
                     TGFEQTLDENYSTAASLLEPVNYKLIQLNFAGTTYIELRPISGPGDNDSFYFINKSPL
                     MSCLALQVPHPLVDTNTPEQASYLVKELSPFIMSVSGHNRCMSSAYSGCSGTTDACGV
                     SDESYRISDPAHSSATYFQKSTEILNEKYSDLNILQLHGFAAEPGDPNFIISNGTRLK
                     PVNDLAWGLGELLSQTLPSSVYPTPSLLTYEAPHKNLSYTKLIGTHNIQGRLMNNYEG
                     DVCSGIQISSSVTGKFTHLEQYNEFRTDKRNYPILRDAVQQIYDCNYIVLAVELEYFH
                     AYCMSGNDVFLEWKSLKENNHSFYTIEESADRETYYAVATFNSNAENNGRYYSYVRED
                     ISVPTYFRLRQTDIYGVSETYNPVY"
BASE COUNT         1344 a          746 c          886 g         1258 t
ORIGIN      
        1 agatttaacc aacaccattt tcttgctgga aaaattgggg aacccgcact ttaaatttaa
       61 agcagttcac atcgccggaa ccaacgggaa agggagtagt gcgcatttta ttgcatcgat
      121 tttacaaagc gcgggttaca atgccggttt atatacttct cctcatttaa aatcttttac
      181 agagcggata aagattaatg gaaaagaagt tagcgaagat tttgttggag agtttgtgga
      241 aagtgttaaa cccttactct cgaaaatctc accgtctttt tttgaaatga ccgtggccat
      301 ggcgtttgat tactttgccc ggaaaaaaat taacattgct gttgtagagg taggattggg
      361 aggccgctta gacagcacca atgtgctgat acctgaggtt tctttaatca caaacatttc
      421 ctacgatcac accgatttat tagggaatac cctggagcag attgcatttg aaaaagccgg
      481 aatcataaag aattcaagac ctgtggttat cggaaagcga caagaggagg ttgcgcatgt
      541 attcagcata aaggctaaaa gtgaaagcac gaggatttac tttgcatccg atgaaataaa
      601 attgaaaccg ctgaacggtg cctaccagat ttttagatcg ggcaaaattt ggattgaatt
      661 gatgaatgta cctattgtaa ctgattatat aattgagaat cttccgggtg tgcttcaaac
      721 tattatttta ctcaggcaga gaggattcat aattaccgat gaagccataa aagaaggaat
      781 ccgggaaatg gtatttaaaa ctggtcttaa gggaagatgg caaaaaataa atgataaacc
      841 ccttgcaatt tgtgatgttg cccacaatct ggatggaatt ctgaaagttt tgaaagaaat
      901 aaggaaaata aaatacaata aattattaat ggtttgggga tgcgtgaaag acaaagatat
      961 tgaacccata ctagctgttc ttccgaagaa tgcaagctac tatttttgcc aaccggatat
     1021 tccacgagcg atggatgcat cattgttaag agaaaaagct gaaaatgtgg gactcaccgg
     1081 cgttgccatt ccaaatgtag aaaaagcttt tcaaagagca gtaagggatg ccagcgaaga
     1141 tgattttatc tttgtaggtg gcagcacatt tgttgttgct gaattggaaa atctataaag
     1201 tggcaagaaa taaacttttg aaatttgcgg ataacaatca aaccgtgaaa gttattcaag
     1261 ctggaaagcc aatttataat tccataaaag gaaattggag aaagcttcag tttaagaaca
     1321 ataatcccat cattcttgaa attgcatgcg gcagggggga gtacactatt ggatttgccc
     1381 ggttatttgc agataggaat tttgttggga ttgatttaaa aggttcgaga ctttgggccg
     1441 gagcgaaaat tgccgataaa gaaaatttaa ggaatgccgc ttttcttcga actcaaatgc
     1501 acatgctgga agatttcttt gaagaaaatg aagtagatga aatatggatt attcacccgg
     1561 atccaagacc aaagaaaagc gataccagga gaaggttaac ttctccaaga tacatggaga
     1621 tatataagcg gattatcaaa cagggtgggt ggattcatct aaaaactgat agtacatctc
     1681 tttacgaata ttctctggaa gttttaatgg accggctgga tatccatggc ctccttcata
     1741 ctgataacct cgaccaatcg acttatttgt cagatcatta tggaattaaa acccgctatg
     1801 aactcatgtt cagagtcgaa ggcgaaaata tcaaatatct caaatttcaa tttcagtgat
     1861 gggaattgct atccctaccg cgtggtgaat ataattggaa attaattttt catatcccga
     1921 caaataatgc tgaatcagaa acaaagcgaa aaccggatgt gattgtcttt tgtttttcaa
     1981 gaagtgcaga taagttcaat aatttttggg taaatgcttc aactttagtt caattcaatt
     2041 ttatggaata taaaaatctt atcaaaatcc aaacattgct aacaccatcc gagggaatat
     2101 gattctgtga aatacccgga taataggtga tttaagattg aaattagcgc aatatttttg
     2161 ttaatgcttt cttccccttt tgtacgagtg aaacagagca aacagcttag ttaggaattg
     2221 tggtgggaac catctaatcc ggcgttgaag ggaaattttt tgaatgacag ggattaaatg
     2281 gtctgcgtga aacggcgaat tgtattgctt tacttcattt ccagtctgat aacgggggtt
     2341 ccttccattt gtcaagttac tatttcgagt tctcccttta attatttgca aaattttgat
     2401 aacctgataa atacggggtc agctccatgg gtggataatt caacacttcc cggatggtat
     2461 gccactgaaa actctgtccg ggcaagcgat ggaaactctc cacagttaac catgtttagt
     2521 tttggtaccg ggacagacag ggcattgggc gccaggcagg ataattcaac aggagagatg
     2581 gcttatggag ttcggttagt aaacaattcc ggcaaaacgt ttcaatcgat gtttatagat
     2641 ctcgatggag aacaatggag gaaatctgct gacagcattc cacaggttat taaatttatg
     2701 tttattatca gcaactctcc gatcaattca atcgatattt caactatcaa taacactgaa
     2761 ttttatgaat tcaatttcat cagtccggtt aataattcat tgcccataac ggcgctggat
     2821 ggaaatttgc cggcaaacag gtatcatctg tcggcatcaa ttaaaattaa ttttccgcca
     2881 ggttatgata tcctgttgaa gtggcttgac ccaaatgata aaacggtgga tcatggaatg
     2941 ggtattgata atttgaatgt agttttttca gaaactatac tggattatga tgtggcaaac
     3001 attagcgcca attttgaaga tttttttaat tcctctatta aatacgaaat cattgacgaa
     3061 actaacctct caacatttac aaatcctgat caactatcta ttgaaacctg gggaaccgga
     3121 tttgaacaaa cattggatga aaattattca acggcggcat cccttttgga gccggtaaat
     3181 tacaaattaa ttcagctgaa ttttgcgggg accacctata tagagcttcg tcctatctcg
     3241 ggtccaggtg ataatgatag tttttacttc ataaataaaa gtccattaat gagttgcctt
     3301 gctttgcaag ttccacaccc gttagttgat actaatactc ctgaacaggc gagttattta
     3361 gtaaaggaat tgagtccatt tatcatgtcg gtatccggac acaacaggtg catgtcatca
     3421 gcttattccg gttgcagtgg tactaccgat gcttgtggag tttctgatga atcctacagg
     3481 atatccgatc cggcacacag ctccgcgact tattttcaga aatcaacgga aattttgaat
     3541 gagaaatatt cagatttgaa tatcctgcag cttcatggct ttgctgctga gccgggagat
     3601 ccaaatttta tcatttcaaa tggtaccaga cttaagccgg taaacgacct ggcctggggg
     3661 ctcggtgaac ttttatcgca gactcttcct tccagcgttt atccaacgcc aagcctgctt
     3721 acttacgaag ctccacacaa aaatctttct tatacgaaac tcattggaac tcataatatt
     3781 caggggcgat taatgaataa ctatgaagga gatgtgtgtt cgggcattca gatttcctct
     3841 agtgttactg gtaaatttac ccatctggaa cagtataatg agtttagaac agataaacgc
     3901 aattatccta tattacgtga tgcagttcaa caaatctatg actgcaacta tattgtactt
     3961 gctgttgagc ttgaatattt ccatgcttat tgcatgtccg gaaatgacgt ttttttggaa
     4021 tggaaaagtc ttaaagaaaa taaccacagc ttttatacaa tcgaagaaag tgcggacagg
     4081 gaaacatatt acgccgtcgc tacatttaat tccaatgcag agaataatgg gaggtattac
     4141 agttatgtcc gtgaggatat ttctgtaccg acatactttc gtttacgcca gacagatatt
     4201 tatggagtaa gtgaaacgta taatcctgtt tatg
//
LOCUS       DASWHX010000242         3853 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_2180857, whole genome shotgun sequence.
ACCESSION   DASWHX010000242 DASWHX010000000
VERSION     DASWHX010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 3853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3853
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2180857"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..732
                     /locus_tag="VGA21_15050"
     CDS             <1..732
                     /locus_tag="VGA21_15050"
                     /inference="COORDINATES: protein motif:HMM:NF023179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HEX9651876.1"
                     /translation="IARKIGISANAFHERVNFLSNTLLNLGFSYKIYQTKYNSLSAGI
                     STELTCIRVRDDTNFEGGNHDPVLLYNNGRLNRADFGLGFYYQSQYIDIMLAAGRISS
                     GWRSESKNLTTNLFASVRGKLPLFLSTDLFEPYVSLTRLSTSGNLISAGFFYTCFDVI
                     SIGIGYTSTDAVAYSLSVSIDENIYVGYSIQDHFKSNGGLLGDSHEVILKVEFNQGSK
                     EKLPMNSKKKQKRNKRLSKFTGKNN"
     gene            complement(741..1166)
                     /gene="rpiB"
                     /locus_tag="VGA21_15055"
     CDS             complement(741..1166)
                     /gene="rpiB"
                     /locus_tag="VGA21_15055"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019595792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HEX9651877.1"
                     /translation="MSKKIAIGGDHAGFELKNFIRDLLIKEHDVKDFGPFTNESVDYP
                     DFAHPLSEAVEKGEFDLGILVCGSGNGVCMTANKHKGIRAALAWNEELASLARQHNNA
                     NVLCLAGRFIDKVSAEKIVKAFLSANFEGGRHETRVNKI"
     gene            complement(1163..1963)
                     /gene="tatC"
                     /locus_tag="VGA21_15060"
     CDS             complement(1163..1963)
                     /gene="tatC"
                     /locus_tag="VGA21_15060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008627869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEX9651878.1"
                     /translation="MSFLDHLEELRWHLVRALIAVGVFSVIAFLLKDFFFGKLILGPS
                     RLDFYTYRLLCEFSDKLGLEALCIKQLPFVIQSRQMAGQFSMHVTSSFVIGLICAFPY
                     AFWEIWGFIAPGLYYNEKKIARGATFFVSLLFMIGVVFGYFIIAPLSINFLSNYQVDS
                     SILNQFDITSYVTTLTMLVLACAFVFQLPIVVYFLTKAGLINAQFMRSYRRHAIVVIL
                     ILGALITPPDPISQVLVSIPLYGLYEISIYISAVVTRRRKKKEALEAL"
     gene            complement(1989..3260)
                     /gene="glyA"
                     /locus_tag="VGA21_15065"
     CDS             complement(1989..3260)
                     /gene="glyA"
                     /locus_tag="VGA21_15065"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013455251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEX9651879.1"
                     /translation="MKRDHEIFSLIEKEKSRQQNGIELIASENFTSPQVMEAMGTVLT
                     NKYAEGLPGKRYYGGCEVVDEVEQLAIDRVKELFGATWANVQPHSGAQANAAVMLACL
                     QAGDKIMGFDLSHGGHLTHGSPVNFSGKLYRPVFYGVQKETGTIDWDRLTDLADKEKP
                     KLIIAGASAYSRDWNYEAMRLAADRAGAILMADIAHPAGLIARGLLDDPLEFCHVVTT
                     TTHKTLRGPRGGMIFLREDIDNPWGLKSPKGKIRKLSSVLDSGVFPGTQGGPLEHVIA
                     AKAVSFKEALSDDYLNYVIQVQKNAHAMAEAFVAKGYSVISGGTDNHMMLIDLRSKNL
                     TGKEAEESLIKADITINKNMVPFDDQSPFVTSGMRIGTPAMTTRGMKENDMRKITDMI
                     DTVLKNKDSESKILEIKQEVNRWSNQFPLYQ"
     gene            3401..>3853
                     /locus_tag="VGA21_15070"
     CDS             3401..>3853
                     /locus_tag="VGA21_15070"
                     /inference="COORDINATES: protein motif:HMM:NF022441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2807 domain-containing protein"
                     /protein_id="HEX9651880.1"
                     /translation="MKKFSIFILAFAMMSCELAYYKRGNGKMITRDFDVRSFSEVELR
                     GDYEITLIESDKERLEIEADENLMDDIDVEVIGDILRIESRRIRSRKGIRINIFYNEL
                     SGLYAGGAIELRSRNPLRSRYFDLNLAGAGEIILEFEGEKLNLRLSGAG"
BASE COUNT         1144 a          816 c          805 g         1088 t
ORIGIN      
        1 atagcccgga aaatcgggat tagtgcaaac gcctttcatg aaagagtaaa cttcctaagt
       61 aatactcttt tgaatttagg attttcatac aaaatttatc agactaaata taattctcta
      121 tccgccggaa tatcaactga attaacctgc attagggtga gggatgatac aaattttgaa
      181 ggtggtaatc acgatcccgt tttgctgtat aataacggca gactaaatcg tgctgatttt
      241 gggcttggat tttattatca gtctcaatat atagatatca tgctggcagc gggcaggata
      301 tcctcgggat ggcgaagcga atcaaaaaat ttaacaacta atttgtttgc atccgttcgt
      361 ggtaaactac cgctattttt atccactgat ttatttgaac cctatgtgtc gctcacgagg
      421 ctatccacgt caggaaatct catttcagcc gggttcttct atacctgctt cgatgtgatt
      481 tcgattggga ttggttatac cagtactgat gcagttgcct acagcctgtc agtttccatt
      541 gacgagaaca tttacgttgg ttattcaatt caagaccact ttaaaagtaa tggtggattg
      601 ctgggtgata gtcatgaagt aatactgaaa gtagagttca atcagggcag caaggaaaaa
      661 ttgcccatga actctaagaa gaagcaaaaa aggaataagc ggctaagtaa gtttactgga
      721 aagaacaatt gattccggac ttaaatttta ttcactcttg tttcgtgccg cccgccctca
      781 aaatttgcac ttagaaaggc tttcacaatt ttttcggcag aaactttatc aataaacctg
      841 ccggcaagac acaatacgtt tgcattgtta tgctgacggg caagggaggc caactcttca
      901 ttccaggcca gcgccgctct aattccttta tgcttgttcg ctgtcatgca aactccattg
      961 ccacttccgc acaccaaaat acctaaatca aattcacctt tctccacggc ttcggaaagg
     1021 gggtgagcga agtcagggta atccacggac tcatttgtaa aggggccaaa atctttcaca
     1081 tcatgttcct taataagtaa atctcttatg aaatttttaa gttcaaatcc ggcatgatcg
     1141 ccaccaattg caattttctt actcataatg cttctaaggc ttccttcttt ttcctgcgtc
     1201 gtgttactac agcggaaata tagatagata tttcataaag gccataaaga ggaatagaaa
     1261 caagtacctg gctaatagga tccggcggag tgataagagc accaagaatc aggatcacaa
     1321 caatagcatg gcgccggtaa gacctcatga attgtgcatt aattaatcct gccttggtca
     1381 gaaagtaaac gacaatagga agttgaaaca caaatgcaca tgcaagtacc agcatggtga
     1441 gtgtagttac ataagaagta atatcaaatt gattcaatat tgaagaatca acctgatagt
     1501 ttgaaagaaa atttattgag agaggtgcaa taataaagta gccgaatacc acgccaatca
     1561 taaacagcag gcttacaaaa aatgtagccc cacgggcaat ctttttttca ttatagtata
     1621 aaccaggtgc aataaatccc catatttccc aaaacgcgta agggaaggcg caaatcaaac
     1681 cgatcacaaa actggaagtt acgtgcatgg agaattggcc ggccatctga cgactttgta
     1741 taacaaaggg aagttgctta atacacaatg cttccaggcc caacttgtca ctaaactcgc
     1801 acaatagccg gtaggtgtaa aaatcaagtc gggaagggcc aaggattagt tttccaaaga
     1861 aaaaatcttt caaaagaaat gcgattacgg aaaatacgcc taccgcaatc aaagcccgga
     1921 ctaagtgcca cctgagttcc tcaaggtgat ccagaaacga catctctttg gaatttttct
     1981 ttgccacttt attgatacaa tggaaactga ttactccacc tgttcacttc ctgcttaatc
     2041 tccagaattt tgctttcact atctttattt ttaaggacgg tgtcaatcat atctgtaatc
     2101 ttgcgcatat cattttcttt catgccgcgg gtagtcattg ccggagttcc gatcctcatt
     2161 cctgaagtta caaacggtga ttggtcatca aaaggaacca tatttttatt aatggtaata
     2221 tccgctttta tgagtgattc ttccgcttct ttccctgtta agttttttga ccgcaaatcg
     2281 atcagcatca tgtgattatc cgttccaccg gaaatgacag aatatccttt agccacaaaa
     2341 gcttcggcca ttgcatgcgc gtttttctgc acttgaatta cataattcag ataatcatca
     2401 ctcagggctt ccttaaatga aacggctttt gcggcaatca cgtgttcgag cgggccacct
     2461 tgtgttccgg gaaacacgcc ggaatccaaa acagaagaaa gtttccgaat ttttcccttt
     2521 ggagacttta gtccccaggg gttatcaata tcttcccgga gaaaaatcat acccccgcgc
     2581 ggaccacgca atgttttgtg ggtggttgtt gttacaacat ggcaaaattc gaggggatca
     2641 tcaagcaaac cgcgtgcaat taatcccgcg ggatgggcga tatccgccat taaaatagcg
     2701 ccggctctat cagcagccaa tcgcatagct tcataattcc agtcccggct gtaggcgctg
     2761 gctccggcaa taattagctt tggtttttct ttgtccgcaa ggtccgtcag gcggtcccag
     2821 tctatggttc cggtttcctt ttgaactcca tagaaaaccg gacggtaaag tttgccggaa
     2881 aaattaacgg gtgaaccatg agtgagatga ccaccgtgtg acaaatcaaa tcccattatc
     2941 ttatcgccgg cttgtaagca agcaagcatc acggcagcat tggcttgtgc gcctgagtgc
     3001 ggttggacat tcgcccaggt tgcgccaaat aattctttca cccgatcaat cgcaagttgt
     3061 tccacttcat ccacaacttc acagccaccg taatatctct tgccaggcaa tccttcagcg
     3121 tacttgtttg taaggacagt acccatagct tccataacct gcggggaagt gaagttctca
     3181 gaagcaatta attcaatccc gttttgctgc ctggatttct ctttttcaat aagggaaaaa
     3241 atttcatggt cacgcttcat gagcctgtgt tttgaatcgt caaaattagt ttaagggtga
     3301 ttaattacca attatctgaa attgaaccgg ataatccttt tattatcacc cattcgataa
     3361 tatttcgtat attcattaaa ctcccaaatt caaaaatgtt atgaagaagt tttcaatatt
     3421 cattcttgcc ttcgcaatga tgagttgcga attggcctac tacaagcggg gcaatgggaa
     3481 aatgattacc cgcgattttg atgtcagatc attcagcgaa gttgaacttc gcggagacta
     3541 tgagattaca ttaattgaaa gtgataagga aagattggag attgaggctg atgaaaatct
     3601 gatggatgac attgacgtag aggtaattgg ggatatactc aggatagaaa gcagaaggat
     3661 cagaagccgg aagggtatac gtattaacat tttctacaat gaactgtccg gcctttatgc
     3721 aggcggcgcg atagaactta ggtcacggaa tccgctcagg tcgagatact ttgatctcaa
     3781 tcttgccggt gcaggagaaa ttatcctgga atttgaagga gagaaactaa acctgaggct
     3841 gagtggcgcg ggg
//
LOCUS       DASWHX010000243         4524 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_3270906, whole genome shotgun sequence.
ACCESSION   DASWHX010000243 DASWHX010000000
VERSION     DASWHX010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 4524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4524
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3270906"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(175..489)
                     /locus_tag="VGA21_15075"
     CDS             complement(175..489)
                     /locus_tag="VGA21_15075"
                     /inference="COORDINATES: protein motif:HMM:NF025687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 family protein"
                     /protein_id="HEX9651881.1"
                     /translation="MLNPAINIILNPSKIGVISILFASNLLTYIIDIVLIWALYILLK
                     PINEKLALLAVFLRLMETACLAMGVTDELVTLRLLSNPTYSAMMEPNQLYAFARMLPV
                     LV"
     gene            complement(859..3630)
                     /locus_tag="VGA21_15080"
     CDS             complement(859..3630)
                     /locus_tag="VGA21_15080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020601117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 metallopeptidase family protein"
                     /protein_id="HEX9651882.1"
                     /translation="MKHLSITFSLVIFLIHPVCSQNHETDYESKIKEFTTDTRFFAES
                     VGSIFDHPSIPSPLDHFGTIIGAAGVMHRTSEIYGYYKALSDASPNLIMDQSGTTEEG
                     RPFYLITISSQSNITNLVRYKDILSKLADPRKIDKETADQLARDGKPVYYLNGGLHSP
                     EMGSPEMLMELAYRLVTDPSDEIKNILENSIVLINPVSEPDGRDKQVDWYYRYSKERT
                     DWNDGFTRSPPYWGKYVYHDNNRDGLQVSQELTKNIFRVFFDWHPTIMLDLHESVPLL
                     YISTGTGPYNENVDPITIGEWQAISNHEMTALAAQGMPGVFNWAFYDGWWPGYGIWIA
                     NNHNSIGRFYETFGNAGANTYLRDISRQRIAGDLITSREWYRPDPPSGKVFWSARNNI
                     NYMQAGVLASLTYAADNGATLLKNFYKKGVNNVMTGEKGKVKMFAIPAKQRDPAMAAY
                     LVNQLQNQGIEVHRVKDGEYKNEYAVLLDQPYSKLAVDLLTKQNYPQDAKFPPYDDIA
                     WTLGYLYGVEVQAIDSMKYDRSRLTLLSGLEQYKGNVTGEGVDYILPYKAQSGVLTAI
                     YDAKNRSKKMKAYVVNKKTASGNDTLNTGTIILKGLTSAQAQELAAKHGLDLMAGNAG
                     IDNAHEISLPRIAVYHSWYNTQDEGWVRFTLEQKGIPYTSIDKDDLKTGKLRSLFDVI
                     LIPSQRGGATGFINGLNDAFGPMAYTKTEQYPSHGTPDATSDMTGGPGHTGVGNLANF
                     IREGGVLIPLEASAISIADLGIGRQVSSFSPGGLFHPGSIVTVKSRNNTSPILYGFPE
                     TFHVFKGNGELLSTAIYNRNLMILQYGTKALEDEMEYTGEIMGMKKPEDEKKEATEKK
                     TEKSTPYVLSGMVRNEKAIIGQGAIFNVPYGSGRVVFFTFNPLHRFLNHHDSSLLWNV
                     LINWNYLDK"
     gene            4294..>4524
                     /locus_tag="VGA21_15085"
     CDS             4294..>4524
                     /locus_tag="VGA21_15085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651883.1"
                     /translation="MGEIRNKPKIKLKIIKEGEGYTAVGQWRNRSLVTCGDTWDELQT
                     MILEMVNLSFEDLGYTFTIEEIQFEYDLESFFD"
BASE COUNT         1329 a          903 c          945 g         1347 t
ORIGIN      
        1 aaaatttcga agataagtcc aatcctataa ctttttaatt atgacctgaa aattgaagtt
       61 gccgaacccc ttcgctgctt gtgcggaaat attaacgcct ttataagaac cataatccca
      121 atgtaacttc aaaaatactg aaaactgttg aacccaaacc aggaaacgta aatcctatac
      181 taaaactgga agcattcttg cgaacgcgta gagttgattt ggttccatca tggctgaata
      241 cgttgggttt gacagcaacc gtaaggtaac cagctcatcg gttactccca ttgccaggca
      301 agcggtttcc atcaatcgta aaaatactgc taacagggca agtttttcat taatgggttt
      361 gagtagaata tacaatgccc agatgagaac tatatcaata atatacgtaa gcaaattgct
      421 tgcaaacagg attgaaatta ctcctatttt actgggattt agtattatgt taattgccgg
      481 attgagcata ttaataatat ggtctcagac agtaaaagta gcaatcacaa aacctgtgaa
      541 gtggcctgag gcatgaataa ttaaagtggc tacatactac attagcatta atatttatcg
      601 agttggtgtc ggtcttcact ttgggctgtg ggaattttgc tcttgccata gcttgagttt
      661 cgcggtgggt ttccttcggc aagctcagga caagtgcacg gctatggtgt cgcgtgcagg
      721 tttttctgtt gaatttcatt tcagtgattg caacaaccat tgtaatgtat gtgctggcag
      781 ttcctattcc tggaaggtca gatggaattc ccgattgggc acattaaatt acaatatctg
      841 agaaattcta tttgagattc atttatccaa ataattccag tttatcaata cattccaaag
      901 caaagaagaa tcatgatgat tcagaaatcg gtgcaatgga ttaaaagtaa aaaagaccac
      961 tcgtccgctt ccataaggta cattaaaaat tgctccttgt ccgataattg ctttctcgtt
     1021 tcgaaccatt ccggataaca cgtaaggagt acttttttca gttttctttt ccgttgcttc
     1081 ctttttttcg tcttcgggct ttttcatccc cataatttct cccgtgtatt ccatctcatc
     1141 ctccagcgcc ttggttccgt actgcaggat catcaggttt ctgttataga tggcagtgct
     1201 gagcagctca ccatttccct taaaaacatg aaaggtttct ggaaatccat aaagaatagg
     1261 gcttgtatta tttcttgact tcacggtaac aattgaacca ggatggaata atcctccggg
     1321 actaaaactg gaaacctgcc tgccaattcc aaggtctgca atgctgatcg cggatgcttc
     1381 aagaggaata aggacgccac cttctctaat gaaattagcc agattgccaa cgccagtatg
     1441 acccggacct ccggtcatat cagaagttgc atcaggcgtt ccatgagatg gatattgctc
     1501 cgttttagta tacgccatcg gaccaaatgc atcattcaat ccattgataa atccggttgc
     1561 tccacctcgc tgactgggga tcaggattac atcaaataaa ctcctgagtt ttccggtttt
     1621 tagatcgtcc ttatcaatag aagtataagg aatacctttt tgttccaggg taaatcgtac
     1681 ccacccttca tcctgtgtat tataccagct atgatataca gcaattcttg gcaacgaaat
     1741 ttcatgagca ttgtctatgc ctgcattgcc agccatcaaa tccagtccgt gttttgcagc
     1801 taactcttgt gcctgagcag aagtcagacc tttcagaata atggtgccgg tatttaaagt
     1861 atcgtttcct gaagcagttt ttttattaac cacataggcc ttcattttct tactgcgatt
     1921 tttggcgtca taaatggcag taagaacgcc ggactgtgcc ttgtatggta gtatgtaatc
     1981 tacaccttca cctgttacat tacccttata ttgctcaaga ccggatagca gagtaagtct
     2041 actcctatca tatttcatag aatcaattgc ctgcacttcc acaccgtata agtaccccag
     2101 agtccatgcg atatcatcgt agggaggaaa tttcgcatct tgcggataat tttgtttggt
     2161 taacagatct accgcaagtt tgctataggg ttgatccaga aggacagcat attcattctt
     2221 atattctccg tccttaaccc gatgaacttc aattccctgg ttttgaagct gattaacaag
     2281 gtatgcagcc atagcggggt cccgttgttt tgcgggaata gcaaacattt ttactttccc
     2341 tttttcaccg gtcatgacat tattaacacc tttcttataa aagtttttta gaagagtagc
     2401 accattatcg gctgcatagg ttaatgatgc cagaacccct gcctgcatat aattaatatt
     2461 atttctggca gaccaaaata cttttccgct cggagggtca gggcggtacc attcgcgtga
     2521 ggtaatcagg tctccggcaa tccgctgcct gcttatatct ctcaaataag tgttggcacc
     2581 cgcgttgccg aatgtttcgt aaaaccggcc gattgaattg tgattattgg caatccagat
     2641 tccgtaacct ggccaccagc cgtcataaaa tgcccaatta aaaacaccag gcattccctg
     2701 ggctgcgagt gcggtcattt catgattgga tatggcctgc cactcaccaa tagtgatcgg
     2761 atccacattt tcattgtatg gtcctgttcc ggtggagata taaagaagcg gaaccgattc
     2821 atgcaggtcg agcataatgg tgggatgcca gtcgaaaaat actctgaaaa tatttttggt
     2881 tagttcttgc gaaacctgaa ggccatcccg gttattatcg tggtaaacat attttcccca
     2941 gtagggagga cttcttgtaa acccgtcatt ccaatcggtg cgttcttttg aataccggta
     3001 ataccagtct acctgtttat cgcgtccgtc cggttcagaa accggattta tcaaaacaat
     3061 ggaattttca aggatatttt taatttcatc cgaggggtct gtcaccaatc ggtatgcgag
     3121 ttccatgagc atttcaggag atcccatttc cggtgagtgc aagcctccgt tcagataata
     3181 gacaggtttt ccatcgcggg ctaactgatc tgcggtttct ttatcaattt tcctcgggtc
     3241 agcaagtttg ctgaggatgt ctttataacg cacaaggttg gtgatattgg attgactgga
     3301 aatcgtaatt aaataaaaag gccggccttc ttccgtggtt cctgattgat ccataattag
     3361 atttggagac gcatcggaca gcgctttgta gtatccgtaa atttctgatg tgcggtgcat
     3421 cacaccagct gcgccgatga tcgttccgaa atgatctaat ggggaaggaa tagaaggatg
     3481 atcaaaaatt gaacctactg attctgcgaa aaatcgggta tcggtggtaa attctttaat
     3541 cttactttca taatccgttt cgtggttttg actgcaaaca ggatgaatca aaaatatgac
     3601 taaggaaaag gttattgaaa ggtgtttcat ttggttgtgg ctcatggtat taaggggata
     3661 aaattgaaaa tcgaatataa agaaaatagg cttttaatgc ctaccgcgcg taaaaaaaat
     3721 cgaggattaa gaagttctgg taagcagtta tttaggaatt tttgttgaag cccagtgaac
     3781 gtgaataact tcaaaatatg attttggaaa ttgctaatct tacttttgaa aatttgggtt
     3841 catcaaaatg acgcgctgct aaatttccag cgctcggttc caggtataat tacaaattaa
     3901 tcaatcaaag aaaactaaaa actataatgt agggaggaga agttatttca aattttggga
     3961 gaggagatag aaaatgtgtt tcaaccggag gagaaaaaaa tgaagtcgaa aaaaatccgg
     4021 agggctaaca gccattttgt tcggtaaatt tccccggaat taagtaatga atttattacc
     4081 tttgtatttg tgaaaagcag tgaattgcta aggatcatga aaagggacgg atggtatgaa
     4141 ataaggaaaa agggcagcca tattattatg caacaccctg ataagccaaa tatcataccc
     4201 gtgcccttcc atgcctccaa agaaatgaaa aaaggcactt tacaacacat tttaaaactg
     4261 gcagaaattg aaacccggaa aaggtaaaac gatatgggag agattagaaa caaacctaaa
     4321 atcaagctga agatcattaa ggaaggtgag ggatatacgg cggtaggtca atggagaaac
     4381 cggagccttg ttacttgcgg agacacctgg gatgaacttc aaacgatgat cctggaaatg
     4441 gtgaatcttt catttgaaga tttgggctat acctttacca ttgaggaaat acaatttgaa
     4501 tatgaccttg aaagcttctt tgac
//
LOCUS       DASWHX010000244         2652 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_3273017, whole genome shotgun sequence.
ACCESSION   DASWHX010000244 DASWHX010000000
VERSION     DASWHX010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 2652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2652
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3273017"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..435
                     /locus_tag="VGA21_15090"
     CDS             <1..435
                     /locus_tag="VGA21_15090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010609983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX9651884.1"
                     /translation="VKPKASPAQPVHVEDYMSRKLVTFHPDQTMDDVIDTLLKKGISG
                     GPVIDDDGNLVGIISEGDCLKQVVKGKYTNTPNLSGYAKDHMARNVKTVSPQMNIFDL
                     AQMFLDMRLRRFPVIKDGKLVGQISQRDVMKAVQQLKDTTWK"
     gene            501..1445
                     /locus_tag="VGA21_15095"
     CDS             501..1445
                     /locus_tag="VGA21_15095"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9651885.1"
                     /translation="MDSRKKILVFGGTGFIGSEFVKILMANRDAYEIYLLMHTRGKFR
                     EYEGIKIITGNLSGFNLSWLKKINPDYIVHMARMQGRGKLGRFLAAKRGANANKRIIN
                     YLLKNNLHPRIIYVSGSLVYGSVGEKIVREDQQLSPTSFAEQYILAERPWMECLKNKS
                     LPVSIVRPPWIVGQGSWFKHFFLDQIKYESKVPLIGEGNNWMSVISLQDCAGLIYHCL
                     NAELNNIFFNICMPGIYIKMKDFVAIISESVTIPVQSYSTQQAKKLFGAAAAAAFQTS
                     VRLHTNYEDIYLGYPWQHFDIESIIKSNLRIPVNHSEV"
     gene            1442..2605
                     /locus_tag="VGA21_15100"
     CDS             1442..2605
                     /locus_tag="VGA21_15100"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX9651886.1"
                     /translation="MKLLILSPQFPSPEFPMAGKSKSQLAIELTKLGVQVEIIVPVAF
                     SLKFITTLFHIESEAHDNVITHHPIYIRLPFVERFYFLLNPLQRWLFYMAVRKRIKQY
                     ISQGGKIIHAHSMLFAGYVANKFKASNNCKSIVTFHDNEIQSLSGNIRAYRTFLETEI
                     NKSDLIIFVSKNLRNNYFALAEARINSEIIPFPIDHYKFEKSLSKPFTIVGCGRLIPS
                     KGFEILIAAAKLLYLAGEEVKIRIIGEGPEKQNLQNLVRQSGLEKFVRFKGFLSNSEV
                     IAEMSAAHLFILPSYRESLGLVYLEAMSVSTPVIGVKGQGIEDFIVNGENGYLMDAED
                     TGQLYSLIQKLMNNDNLQVRIGKNGFETYRNSLANVRNNAAYHFKLYERFLNE"
BASE COUNT          847 a          476 c          552 g          777 t
ORIGIN      
        1 gtcaaaccca aagcgagtcc ggcacaacca gttcatgtag aggattacat gagcagaaag
       61 cttgttacct tccatcctga ccaaaccatg gatgatgtaa ttgatacctt gttaaagaaa
      121 ggaatttcgg gcggccctgt aatagatgat gatggtaatt tggttggaat aatttctgaa
      181 ggggactgcc tgaagcaggt tgttaagggg aaatacacca atacaccaaa tctttcggga
      241 tacgcgaaag accatatggc tagaaacgtg aagaccgttt caccgcaaat gaatatattt
      301 gatcttgccc agatgttcct ggatatgaga ttacggaggt ttcctgtgat aaaggatgga
      361 aagcttgtcg gacaaatcag ccagcgggac gtaatgaagg cggttcagca attaaaagac
      421 acaacctgga agtaaacacc agtttgaact aacaaaaaaa actctcaatc ttgggagttt
      481 tttttatgat tacaaatact atggattcaa ggaaaaaaat tctggttttt ggcggcaccg
      541 gatttatagg ttcagaattt gtgaaaattt tgatggcaaa cagggatgcc tatgaaattt
      601 atcttttgat gcatacaagg ggtaaattca gagaatacga aggcatcaaa attattacag
      661 ggaatttatc ggggttcaat ttgtcctggc tgaaaaaaat aaacccggat tacatcgttc
      721 atatggccag gatgcagggg agagggaaac ttggaagatt tcttgccgct aagagaggtg
      781 caaatgctaa taagagaatt atcaattatt tactgaaaaa taatcttcat cccagaatta
      841 tttatgttag tggcagcctg gtttatggat ctgttggaga gaaaatagtt agagaggacc
      901 aacaattatc tccaaccagt tttgctgaac aatatattct ggccgagcgt ccctggatgg
      961 aatgtcttaa gaataaatct ttaccggttt ccattgtaag acctccctgg attgtaggcc
     1021 agggttcctg gtttaaacat ttctttcttg accaaataaa gtatgaatcc aaagtaccgc
     1081 ttatcggtga aggaaataat tggatgtcgg ttatttccct gcaagactgt gcaggactaa
     1141 tttatcattg cctcaatgca gaattaaata atattttctt caacatctgt atgcctggca
     1201 tatatattaa gatgaaagat tttgttgcaa ttatttctga atcagtaact attcctgttc
     1261 aatcttattc aacccaacag gcaaagaaat tatttggagc cgcggcagct gctgcctttc
     1321 aaacctcggt caggttacat actaattacg aagatattta cttgggttac ccctggcagc
     1381 atttcgatat agaaagcatt attaaaagta atttaagaat accggtaaat cattcagagg
     1441 tttgaaatta cttattctgt cacctcaatt tcccagtccg gaatttccaa tggctggcaa
     1501 atctaaaagc caattggcca tagaacttac taaactcggt gtgcaggttg aaatcattgt
     1561 ccctgtagca ttttcgctaa aattcatcac aactcttttc cacattgaaa gtgaggcgca
     1621 tgataatgtc ataacccacc atcctattta tattagattg ccatttgttg agcgatttta
     1681 ctttctgctt aaccctctgc aacggtggct attctacatg gctgtaagga aaaggataaa
     1741 acaatatatc agtcagggcg ggaagattat tcatgcacac tccatgttat tcgcagggta
     1801 tgtagcaaat aaatttaaag catcgaataa ctgtaaatcc atcgtaacct ttcatgataa
     1861 tgaaattcaa agcttatcgg gtaacatacg ggcttaccgg acatttcttg aaacggaaat
     1921 aaacaagtca gatttaataa tattcgtatc taaaaacctg aggaacaatt actttgcgct
     1981 agccgaagca aggattaata gtgaaataat tccctttcct attgatcact acaagtttga
     2041 gaaaagttta agtaaaccct ttaccatagt tgggtgcgga agactaatac ccagtaaagg
     2101 ttttgaaata ctgatcgccg cggcaaagct actttacctg gccggggagg aagtgaaaat
     2161 cagaattatc ggtgagggac ctgagaagca aaatcttcaa aaccttgttc gtcaatccgg
     2221 gttagagaaa tttgttcgct ttaaaggatt tttgagtaac tctgaagtaa tcgcggaaat
     2281 gtcagctgct cacttgttta ttttaccgag ttaccgtgaa tctcttggcc tggtatacct
     2341 tgaggctatg tctgtctcta caccggttat tggtgttaaa ggtcagggta ttgaagattt
     2401 tattgttaat ggtgaaaatg gatacctaat ggatgcggaa gatacggggc agttatactc
     2461 attgatacag aaacttatga ataacgacaa tctgcaggtc cggataggaa aaaatgggtt
     2521 tgaaacatat agaaattccc tggctaatgt caggaataat gctgcctatc actttaaact
     2581 ttacgagcgt ttccttaatg aataaagaaa atattcgccg aacttccagc aaaacgttct
     2641 atttaccatt cg
//
LOCUS       DASWHX010000245         5352 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_3275691, whole genome shotgun sequence.
ACCESSION   DASWHX010000245 DASWHX010000000
VERSION     DASWHX010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 5352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5352
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3275691"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..482
                     /locus_tag="VGA21_15105"
     CDS             <1..482
                     /locus_tag="VGA21_15105"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEX9651887.1"
                     /translation="LIFESARWAPSSFNEQPWRFIYAHKSNPRDHGKIADALNDHNRS
                     WAAKAPVLIAVIAKTRLSHNERENRHAFYDVGQAVATLTLQATDLGLCMRQMAGFHSD
                     IAIANLGILQGFEPIAIIALGYEGEVDENTDERLAWKQNHVRTRKPVDEVVKEGSWS"
     gene            493..945
                     /locus_tag="VGA21_15110"
     CDS             493..945
                     /locus_tag="VGA21_15110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017732785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX9651888.1"
                     /translation="MKLEDEINQTKFRSEYQKLNLNLIFTNNWVQNKHRKTFEEFGVT
                     PQQYNVLRILKGNYPKPYSTSEIRMRMLDPMSDASRIVERLIQKKLVERKTCKSDRRL
                     VDVVITQKGLNLIEYTSSAIAAQEKIFHALTITEAQDLNTLLDKLRAE"
     gene            complement(1334..2710)
                     /gene="hutF"
                     /locus_tag="VGA21_15115"
     CDS             complement(1334..2710)
                     /gene="hutF"
                     /locus_tag="VGA21_15115"
                     /EC_number="3.5.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009580536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050416 - formimidoylglutamate deiminase
                     activity [Evidence IEA];
                     GO_process: GO:0019557 - histidine catabolic process to
                     glutamate and formate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamate deiminase"
                     /protein_id="HEX9651889.1"
                     /translation="MNLRFSGILTSKGWLTPGYVTINNSGVIEKISDSPLQSEIDEQI
                     NGFALPGFRNSHSHAFQYAMAGITEQHKPGIQDNFWTWREAMYQVALTMNPEQMEKVA
                     TFLYAEMLRHGYTHVAEFHYIHHDKDGSQYDNPAELGERLIAAAATAGIKITLIPIFY
                     QMGNFGQPPQERQRRFISQNYDAYMKLWEASQVAALNYKKASLGVGVHSLRAVRDDAI
                     IRVTENYDPRYPFHLHVSEQLKEVEDCIAHLGVRPARWLLDNVQLDENFHLIHATHLD
                     DTEVKGLAESGANVVLCPSTEGNLGDGIFRLLDFKIFGGKWSIGTDSHIGLNPFEELR
                     MLDYRERLITHQRNVLNDKLFGDTGAYGFDKVLQSGRMAMGDSTTGYLDPGNALDAFV
                     VEDSHPLLASNKPEFLLSTIIYTFDSSQMLGTMVDGKWAIRGQHPQYEKLSRDFLEAV
                     KEMGIRNR"
     gene            complement(2707..3516)
                     /locus_tag="VGA21_15120"
     CDS             complement(2707..3516)
                     /locus_tag="VGA21_15120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009580537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HEX9651890.1"
                     /translation="MKVFEVISPATKPVPVLISVPHCGTGFPEEIKSLIRLELLPPDD
                     TDFFVRQLYDFAPALGITMIYANYSRWVIDLNRDPESKPLYSDGRIITSLVPETDFLG
                     NSIYNNETPTKAEIHRRIKSYYIPYHSEISNQIKALQKKFRHILLFDAHSIRKSVPSI
                     QSQPFPDLILGDNQLKSARSEIIEVVWNEINNSNYTSSHNVPFQGGFITRSFGNPNRN
                     IHALQLEMTKINYMDDSETEYHSGRAAEMRKVLKSIFRKLIVTLEQLNKKE"
     gene            complement(3560..3700)
                     /locus_tag="VGA21_15125"
     CDS             complement(3560..3700)
                     /locus_tag="VGA21_15125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651891.1"
                     /translation="MREQCQELKNKNERFITGGYEKSGRYDFVPYQFALYSGADWFMK
                     GN"
     gene            complement(3791..4981)
                     /locus_tag="VGA21_15130"
     CDS             complement(3791..4981)
                     /locus_tag="VGA21_15130"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX9651892.1"
                     /translation="MTSQSQMNLDAVRNATPGTRKFIHFNNAGSSLPPLPVIEAVNQY
                     NAEEFMRGGYETFELNKDSFHKTYNSVADLINCNPSELALMENATSAWNAAFLSINFE
                     PGDIILATETDYASNYLAYLRLSERIPLEIKILPVEKSGEVSVGELKSMLSSKVKLVS
                     VTHMPTNGGLVNPVEEIGATLKNSSAFYLVDACQSAGQYPLDVKKIGCHFLSATGRKY
                     LRAPRGTGFLYASKATLERTKPITLDLHSAIWTSLDSYVMEPSAKRYENWEFNYSNIF
                     GLKAAAEYAVELGLDNIWERVQKLGQTLRDELSRIDGIIVQDRGSVQSGIVTFTSDKV
                     EYTKLKKKLAVKDIHVGITPKAAAFLDMENRGLSSMIRSSVHYYNSETEIEKFIRVLH
                     DLHS"
     gene            complement(4978..>5352)
                     /locus_tag="VGA21_15135"
     CDS             complement(4978..>5352)
                     /locus_tag="VGA21_15135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651893.1"
                     /translation="EACSRRLPFFNALDGKPREDYSFNYLIISLKFDVTYPYIASLFY
                     NGFTSFRPEIITIINEKFLNLVPQNYLVPQIPLLALATANFIDLVSSKGIDLGEIILS
                     KSSISANSSEILQIIQKNNLLL"
BASE COUNT         1619 a         1098 c         1091 g         1544 t
ORIGIN      
        1 atttgatctt tgaatcagcc aggtgggcgc catcgagttt taatgagcag ccctggaggt
       61 ttatttatgc tcataaatct aatccgcggg atcatggaaa aattgcggat gcattgaatg
      121 atcataatcg ttcctgggcg gcgaaagcac ctgtcctgat agcggtgatt gctaagacga
      181 ggttaagtca taacgaaagg gaaaacagac atgcatttta tgatgtagga caagcggtgg
      241 caactctgac attgcaagct acggacctgg gattgtgtat gcgtcagatg gccggattcc
      301 attcggatat agcaattgca aatcttggaa ttctgcaagg atttgagccc attgccataa
      361 ttgcgcttgg atatgaaggg gaggttgacg aaaatacgga tgaaagacta gcctggaaac
      421 aaaatcatgt gcgcactcgt aagccggtgg atgaagtagt taaagaaggg agttggagct
      481 aaaagaaaca aagtgaaatt ggaagacgaa attaatcaaa caaaattcag gtcggagtat
      541 caaaagctca acctgaatct aatcttcaca aataattggg ttcagaataa gcatagaaaa
      601 acatttgaag aatttggagt tactccgcag cagtacaatg ttttgcgaat tctgaagggt
      661 aattacccga aaccatattc cacttcagag atccggatgc ggatgctgga tccaatgtcg
      721 gatgcatccc gaattgtaga aagactgatt caaaagaaac tagtggaaag gaaaacctgc
      781 aaatcagatc gaagattagt ggatgtggtg atcactcaaa agggactgaa tttgattgaa
      841 tacacctcaa gtgccattgc agcccaggaa aaaattttcc atgctttgac aataactgaa
      901 gctcaagatt taaatacact actcgacaaa ttaagagctg agtagggagg tggtggtcta
      961 agagttaagt taatgcaatt tgcttcgtaa cttttttctc aggtccttca gctctccctt
     1021 attatggaat cgcctcataa tttggtaaat ttattaagat aagaataaaa ccacccctac
     1081 cccaaccttc ggtcggcagg ccctccttaa aataaggagg gaaaaaattt cttcaaaaca
     1141 aattaaccta ctgattcctg accaaattat tatcagtaaa tacaggcaag aagatgaaag
     1201 caaacaggtg aaccagttga tgctaaattc ccttccttat tttaaccgtt tccccgacca
     1261 aaatgggtgg ttgtctcaga gttaacctac cgattccccg accaaaatag gggtggttgt
     1321 ctagagagtt aacctaccga ttccgaatcc ccatctcttt aacagcttcc aaaaaatcac
     1381 gactcaactt ttcatattga ggatgttgtc ccctaatagc ccattttcca tcaaccattg
     1441 ttcctaacat ctggcttgaa tcaaaggtgt aaataatcgt gctgagcaaa aattctggtt
     1501 tgttactggc caacaaagga tgtgaatcct ccacaacaaa agcatccagt gcgtttcccg
     1561 gatcaagata tccagtcgta gaatcaccca ttgccattct gccggattgt aaaaccttat
     1621 caaaaccata ggcacctgta tcaccaaaaa gcttatcatt caaaacattt cgctgatgcg
     1681 taatcaacct ttcccgataa tccagcatcc gcaattcttc aaaaggattt aatccgatat
     1741 ggctgtcggt gccaatactc cattttccac cgaaaatttt aaaatcaagc agcctgaaaa
     1801 taccatcacc taaattgcct tccgtgctgg gacacagaac tacgttggcc cccgattcgg
     1861 ccaacccttt cacttcggta tcatcaaggt gggtagcatg tataagatga aagttttcat
     1921 ccaattgaac gttatccagc agccatcggg ctggcctgac tcctaaatgc gcgatacaat
     1981 cttccacctc ttttaattgc tcagaaacgt gaagatgaaa tgggtacctc ggatcgtagt
     2041 tttctgttac cctgattatg gcatcatctc ttaccgcccg gagtgaatgt actcccacgc
     2101 ccaatgaagc ctttttataa ttaagtgctg caacctggga agcttcccaa agtttcatgt
     2161 aagcatcata attttgacta ataaatcttc gctgtcgctc ctggggtggc tggccaaaat
     2221 ttcccatttg gtaaaaaatt ggtatcaggg taatcttgat tccggcagtg gcggctgccg
     2281 cgattaaacg ttcgcccaat tctgcagggt tgtcatattg gcttccatct ttatcatgat
     2341 ggatgtagtg aaactcggcc acatgtgtgt aaccatggcg aagcatttca gcatataaaa
     2401 atgtagcaac cttctccatc tgctctgggt tcatggtgag ggcaacctga tacatcgcct
     2461 cacgccaggt ccagaaatta tcctggatcc ccggcttatg ttgctcggta attcctgcca
     2521 tcgcgtattg aaaagcatga gagtgagaat ttctgaatcc tggcaaggca aatccattga
     2581 tttgctcatc gatttcactt tggaggggcg aatcagaaat cttttcaatg actccggaat
     2641 tattaattgt gacgtacccc ggagttagcc accctttgga ggttaaaata cctgaaaacc
     2701 gcaaattcat tccttcttgt ttaattgttc aagtgtaacg attagcttgc gaaaaataga
     2761 tttaagaact ttcctcattt ccgcagcacg gcctgaatga tattctgtct ctgaatcatc
     2821 catgtaatta atttttgtca tctctaattg caaagcatga atatttctat tgggattacc
     2881 aaaagaacgc gtaataaatc cgccctgaaa aggtacatta tgagaactcg tataattact
     2941 attattgatt tcattccaaa caacttctat tatttcagaa cgagcgcttt tcaattgatt
     3001 atctcctaaa atcaaatcag gaaatggttg tgactgaata gaaggcaccg attttcgaat
     3061 cgaatgagca tcaaaaagca gtatgtgcct gaattttttt tgtaatgctt taatttgatt
     3121 tgaaatttct gaatgatagg ggatgtaata gcttttaatc ctccggtgta tttcagcttt
     3181 cgtcggggtt tcattattat atatggaatt gcctagaaaa tctgtctcgg gaaccaggga
     3241 tgtaatgatt ctcccgtcgc tatataaagg tttgctttcc ggatcgcgat tcaaatcaat
     3301 tacccagcgg gaataatttg cgtagatcat tgtaattccg agagctggtg caaaatcata
     3361 caattgccgg acaaaaaaat cagtatcatc gggtggaagc agctccaacc gtataaggga
     3421 ttttatttct tcagggaatc cggttccgca gtgtggaacg cttattaaaa caggaacggg
     3481 ttttgttgcc ggtgaaatta cttcaaatac tttcatagct ggtgaaagca aaagtaatca
     3541 atcattaaat caatcaaact taattcccct tcatgaacca atcagcgccg ctatataaag
     3601 caaactgata aggaacaaaa tcgtagcggc cacttttctc atacccacct gtaataaatc
     3661 tttcattttt atttttgagt tcttgacatt gttctctcat aaaaacaaaa ttctcaccaa
     3721 aaattgcaag cacctgaata tcaccagttt aaaagttatg caatgtgtaa ttgaacgata
     3781 atgaacagtt ttaggagtga agatcgtgca gtactctgat gaacttttca atttctgttt
     3841 ctgaattgta atagtgaaca gaagaccgaa tcatactgct gaggcccctg ttttccatat
     3901 caagaaaagc tgcagctttt ggggtaatac cgacatggat gtctttcacg gcaagcttct
     3961 tcttgagctt tgtatactct accttgtctg aagtaaaggt gacaatgcca gactgcacag
     4021 aaccacggtc ttgaacaatg atcccatcaa tccttgaaag ttcatcccga agagtttgac
     4081 ctaatttctg gacccgctcc cagatattat ccaatcccag ttcaactgcg tattctgctg
     4141 ccgccttaag tccaaaaata ttggagtagt taaattccca gttttcatag cgcttggctg
     4201 aaggctccat tacgtatgag tcaagcgaag tccaaatggc tgaatgcagg tcaagagtaa
     4261 ttggcttggt tctttccagg gtagctttgc ttgcataaag aaatcctgtt cccctgggcg
     4321 cgcgtaaata tttgcgccct gtggcagaaa gaaagtgaca accaatcttt ttcacatcca
     4381 ggggatattg accggcagac tgacaggcat caaccagata aaaagcggaa ctgtttttta
     4441 aggtagctcc aatttcttcc acaggattta ccagcccgcc attggtgggc atatgagtta
     4501 cacttacaag ttttactttt gaagaaagca tagacttcaa ttctccaaca gaaacttcgc
     4561 cgctcttttc aaccggaagt attttgattt caagcggtat tctttcactt agtctcaaat
     4621 aggccaggta gttagatgca taatctgttt cagtagccag gataatgtct ccgggttcaa
     4681 aattgatgga aagaaaagcc gcattccacg ctgaggtcgc gttctccata agggccaatt
     4741 cagaggggtt gcaattaatc aggtctgcga cggaattgta ggttttgtga aaagagtctt
     4801 tgtttaattc aaaggtttca tatccaccac gcataaattc ttcagcattg tattggttca
     4861 ctgcctcaat taccggaagt ggaggtaatg aagacccggc attattgaaa tgaatgaact
     4921 tcctcgtgcc tggagtggca tttcttacag catcaagatt catttgtgac tgactggtca
     4981 taatagtaaa ttattttttt gaattatctg aaggatttca cttgaattcg cgcttataga
     5041 ggattttgaa agaattatct cgccaaggtc aatgccttta gaactaacta aatcaataaa
     5101 gtttgcagtg gcaagtgcca gtaagggaat ctggggcacc aggtaattct gaggcaccag
     5161 gtttagaaac ttttcattaa ttatagtaat tatctcgggt cgaaaagagg taaacccatt
     5221 atagaataat gaagcgatgt aaggataggt aacatcaaat tttaatgaaa taataaggta
     5281 attgaaggaa taatcttctc tgggtttgcc atcaagggca ttaaaaaatg gtaaccttct
     5341 gctacaagct tc
//
LOCUS       DASWHX010000246         4191 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_3612624, whole genome shotgun sequence.
ACCESSION   DASWHX010000246 DASWHX010000000
VERSION     DASWHX010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 4191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4191
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3612624"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1895)
                     /locus_tag="VGA21_15140"
     CDS             complement(<1..1895)
                     /locus_tag="VGA21_15140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651894.1"
                     /translation="MKKISLIIFFFAVISKSMATHIVGGEFELIHLTEYNYRLNLIIY
                     FDNINGNPGAEDPSAEVYIFRKVDGALIQTFVLDQTEDYFVQYSIPECANKFPLETRR
                     LNYSRIIFLNPAVYNDPGGYYVVWERCCRNNIIDNIVNPDQTGQTFILEFPPVVKDGQ
                     QFINSSPILFPPLRDYACMDQFYFVDFQGTDPDGDSIAYSLVVPLNSSTSQALPVPSP
                     PPHPLVNWSFGISLSNIVPGNPTLEISTDGLLTVTPSQTGLFVFEVLAEEFRNGEKLG
                     EVRRDFQMLVVECELPKPPPNLVVKAPGSNTFVSDLPLITFQREDEKCLEFAVTSDPL
                     YQKLRFKARPVNFKGDASGILSIKSGTVGLNDSLRFDVCFPDCPFKEGEPFIIDIIAF
                     DNQCPQGKSDTLRLAVNVEPPINSDPFFTNADKSITKLILEGDTWQLAVEGVDPDKDS
                     LQISVLTDEFNPSDYGMSFSEFVYDKDAGTINTTFKWESKCDVYDFTARNNFIMSLLL
                     EDYDTCLLGNADTIRMNLNILLPPNTNPVISTDLPALEIEKYLDTQLSFNVFGEDTDG
                     DLVVLKAAGDDFKLSDYGIQFPGDSAIGNVQSKFLWNISCDIINLNEKDEFNFFIWLE
                     DRDKCKFP"
     gene            1987..3414
                     /gene="rlmD"
                     /locus_tag="VGA21_15145"
     CDS             1987..3414
                     /gene="rlmD"
                     /locus_tag="VGA21_15145"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014772572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEX9651895.1"
                     /translation="MKYFVRFAVYMKSGSILENIEILSMASEGKCVARVENKVIFVKG
                     VAPGDVCDLKITKSKKKFSEAIPVAFRNTSTHRVDPFCSHFGTCGGCKWQHISYQFQL
                     TSKQKLVEESFAHPVRIEIPEVLPIMESEKQTHYRNKLEYTFSNYRWITNEELESKEK
                     LDRRGLGFHVPGRFDRVVNIQECFLQPEPSNGIRNALRDFALKENLSFYNFKTHSGFL
                     RNLIIRNTTLNEVMLIVQFSEDNKNMIEKCMTFLKEKFPEVTSLQYVINQKKNETFFD
                     QEVKLFHGKDFITEQFENYKYRIGPKSFFQTNSHQALRLYQKVREMASLSGKEIIYDL
                     YCGTGTIGIFLSDKSKSVLSIDSIPEAIEDAKENALINNVNNIEFLSGDLKDFLSENL
                     IERYGFPDVVICDPPRAGMHQKVIENLIKLSPEKIIYVSCNPATQARDIALLINYYEI
                     ISMQPMDMFPHTTHVENIALLHKRS"
     gene            3424..3792
                     /locus_tag="VGA21_15150"
     CDS             3424..3792
                     /locus_tag="VGA21_15150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017731352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651896.1"
                     /translation="MVNDPELNGKYLGTITKDFAMVAETLKEASYQIRKRGFSNYPIF
                     PISKSDPGVGKLLIDKHNAAIAWNIHVTYLDEFIQRKLVDEKKQEDFKKVYKDPDEFC
                     CLFVIDNDFTKFIFIPYPID"
     gene            complement(3789..>4191)
                     /locus_tag="VGA21_15155"
     CDS             complement(3789..>4191)
                     /locus_tag="VGA21_15155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018473735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HEX9651897.1"
                     /translation="EARMDIIHELSDLNVKPTSMIDISDGLASELLHLCKGSKLGARI
                     FEAQLPIDKMTYDTAVEFNLDPITCVLNGGEDYELLFTIKQEDHEKLKNHPDIHFIGY
                     MESAIDTPTLVTKGENEVPLKAQGWNHFRKH"
BASE COUNT         1342 a          848 c          806 g         1195 t
ORIGIN      
        1 ggaaatttac acttatcccg gtcttcaagc cagataaaaa aattgaattc atccttttca
       61 ttcagattaa taatgtcgca ggagatattc cagagaaatt tgctttgaac attaccgatg
      121 gcgctgtcac caggaaactg aattccataa tcagacagtt tgaaatcatc cccggctgcc
      181 tttaggacca ccagatctcc atctgtgtct tcaccaaaaa cattaaacga aagctgggtg
      241 tccagatact tttctatttc caatgccggc aagtccgttg aaataaccgg gttggtgttt
      301 ggtggcagca aaatattaag gttcattcgg atggtatccg cattgccaag caagcacgta
      361 tcgtaatcct ccagcagcaa gctcatgata aagttattcc ttgctgtaaa atcataaaca
      421 tcacatttac tttcccactt aaatgtggtg tttatagtgc cggcatcctt gtcataaaca
      481 aattcactaa aactcattcc ataatctgat ggattaaact cgtcagtcag tacagagatt
      541 tggagagagt ccttatccgg gtcaactcct tcaacagcaa gttgccaggt gtcgccttcc
      601 agaataagct tggtaatgct tttgtcagca ttcgtgaaaa agggatcact gttgattggg
      661 ggttccacat ttacagccaa tcgcaaagta tcagactttc cttgcgggca ttgattatca
      721 aatgcaataa tatcaataat aaatggctca ccttctttga aagggcaatc cggaaaacag
      781 acatcaaacc taaggctgtc attgagccca actgtaccgc tcttgataga taggattccg
      841 cttgcatcgc ctttgaaatt taccggcctc gctttgaatc gtaatttctg gtataacgga
      901 tcagaagtaa cggcaaattc caggcatttt tcatcctccc gctgaaaagt aatcaaaggc
      961 aaatcactga caaaggtatt gcttccagga gctttaacta caagattcgg cggcggcttg
     1021 ggcaattcac attccactac cagcatttga aaatctctgc gaacctcacc cagcttttca
     1081 ccatttctaa attcctccgc caggacttca aaaacaaaca agccggtttg agatggagtg
     1141 acggttaaca atccatctgt gctgatctcc aacgtgggat tgcccggaac aatattactg
     1201 agactaattc caaaagacca gttaactaaa ggatgaggag gtggacttgg aacaggtaac
     1261 gcctgagatg ttgaactgtt caaaggaacc actaaagaat aggcaatcga atcaccatcc
     1321 ggatcagtgc cctgaaaatc cacgaagtaa aattgatcca tgcaggcata atctcgcagg
     1381 ggcggaaaaa gaattggtga actgttaata aactgttgtc catccttaac taccggagga
     1441 aactccagaa taaaagtttg gccggtttga tccggattca caatattatc aataatatta
     1501 ttacggcagc atctctccca tactacataa tatccgccgg ggtcattata caccgcgggg
     1561 tttagaaaaa ttattctgga ataattcagt ctgcgcgttt caaggggaaa cttgttggca
     1621 cattcaggaa ttgaatactg aacaaaataa tcttccgtct ggtctaatac gaaagtctgt
     1681 atcaaggctc cgtcaacctt ccggaaaata tacacttcag cggaggggtc ttctgctccg
     1741 gggtttccgt ttatgttatc aaaataaatt atcaggttga gccggtaatt gtattccgtt
     1801 aaatgtatga gctcaaattc accccctaca atatgagtag ccatgctctt tgaaatgact
     1861 gcaaaaaaga aaattattaa agatatcttt ttcatttaaa tcctttcaaa aacaaagtta
     1921 atatacttga aaccggaaga aattaacttc ttcaaaatcc cccaatttcg ggggtgtaag
     1981 taattcttga aatactttgt taggtttgcg gtatacatga aaagcggctc aatcctggag
     2041 aatattgaaa tcctgtctat ggcatctgaa ggtaaatgtg tagcacgggt cgaaaataag
     2101 gtaatattcg tgaaaggtgt tgctccaggg gacgtttgtg atcttaaaat cacaaaatcc
     2161 aaaaagaaat tctcagaggc catacctgta gcttttagaa atacgtccac gcatagggtt
     2221 gatccatttt gttctcactt tggaacctgt ggaggttgta aatggcagca tatttcatac
     2281 cagttccagc taacaagtaa gcaaaagctt gtcgaggaat ctttcgcgca cccggttaga
     2341 attgaaattc cggaggtcct acctataatg gaatctgaaa agcaaacgca ttaccggaat
     2401 aagcttgaat acactttttc taattacagg tggataacaa acgaagaact tgaaagcaaa
     2461 gagaagcttg accgtcgcgg gcttggtttc catgtgcccg gccggtttga cagggttgtt
     2521 aatattcagg aatgtttctt acagccggaa ccttccaatg gcatacgaaa tgccctgcgt
     2581 gatttcgcct taaaagaaaa tttgtccttt tataatttca aaacacattc cggattttta
     2641 aggaacctga taatcagaaa tactacgctc aatgaggtta tgttaatcgt gcaattctcg
     2701 gaagataata aaaacatgat tgagaaatgc atgaccttcc tgaaagaaaa atttccggaa
     2761 gtaacctcgc ttcaatacgt tatcaatcag aagaaaaatg aaaccttttt tgatcaggaa
     2821 gtgaaattat ttcatgggaa ggattttatt acagaacaat ttgaaaatta caaatacagg
     2881 ataggtccaa agtcattttt tcagacaaac tctcatcagg cattgagatt atatcagaaa
     2941 gtaagagaaa tggcttcttt gagtggcaag gaaataattt atgatttgta ttgtggcacc
     3001 gggaccatcg gtatttttct cagcgataag tcaaaatcag tattatcaat agatagtata
     3061 ccggaagcta ttgaagatgc aaaagagaat gccctgatta acaatgtaaa taacattgaa
     3121 tttctttccg gagatttaaa agatttctta agtgaaaatc tgattgaacg ctatggattt
     3181 cctgatgttg tcatctgcga tcctcccagg gccgggatgc atcagaaggt aatagaaaac
     3241 cttataaaac tttcaccgga aaaaattatt tacgtgagct gcaaccccgc gacacaggca
     3301 agggatattg ccttgttgat taattactat gaaatcatct ccatgcagcc gatggatatg
     3361 ttccctcata caacccatgt agaaaatata gcattgcttc ataaaagatc ataaattgtg
     3421 aacatggtaa atgatccgga gcttaatgga aaatacctgg gcaccattac aaaagacttt
     3481 gctatggtgg ctgaaacttt aaaggaagct tcctatcaaa tcagaaagag aggtttttca
     3541 aactacccga tttttcctat aagcaaatct gatcccggag ttggaaaatt attgatcgat
     3601 aagcacaacg cagctattgc ctggaacatc catgttactt acctggatga atttattcaa
     3661 cgaaaattag tagatgaaaa aaagcaggaa gatttcaaaa aagtctacaa agaccctgac
     3721 gaattttgtt gcctgttcgt gatagacaac gattttacaa aattcatctt tatcccctac
     3781 cctattgatt agtgcttcct aaaatgattc cagccttgag cctttagtgg aacttcattt
     3841 tctcctttag ttaccaatgt gggagtatcg atagcgcttt ccatataacc aatgaaatga
     3901 atatccggat gattcttcaa cttttcatga tcttcttgtt tgatcgtaaa aagtaactca
     3961 taatcctcac caccattcaa aacacaagta atgggatcca aattaaattc aacggcggta
     4021 tcatatgtca ttttgtcaat gggaagctgc gcttcaaaaa ttctggctcc taatttcgaa
     4081 cctttacata agtgcagaag ttccgatgcc agtccatcag aaatatcaat catcgaagtt
     4141 ggcttcacgt tcaaatcact cagttcatga attatatcca ttctcgcttc c
//
LOCUS       DASWHX010000247         4635 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_3278589, whole genome shotgun sequence.
ACCESSION   DASWHX010000247 DASWHX010000000
VERSION     DASWHX010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 4635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4635
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3278589"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3863
                     /locus_tag="VGA21_15160"
     CDS             <1..3863
                     /locus_tag="VGA21_15160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651898.1"
                     /translation="FVPEKTDGNPDTGKQVKASFDIVIDDWNNMLVSLNIDPFQLKSM
                     NGLGFKVTNAVIDMSDTRDPLGVSFPAGYNAGTPPGLWRGVFIRELSIKLPKQFKSND
                     TQSNPQNPRRIEIEGSNMIIDNQGFTGVISANYLLNLDQGAIGKWAFSLNHLHIAFVG
                     NQIESAGFSGAIRIPVTGDRDTLSYSAIIDPGNSYIFTITPPDSVRIPIFNATKVVLS
                     PASSLTIVIEDGEFRPRANLHGVLGIGGDKVALAQIEFENLQVQTVSPYLSIGNLSFG
                     SPALEQKMGNFPIQIHNIGFQNRENNKVALLFEVTVALMKGEDSGGIGGSAGFVIEGK
                     IEDGGNLLDFKYDHLELNKISINADIGAVKLAGTLEWFEEDPIYGKGVKGYAMLEVTK
                     ISLQATVLFGKINGFRYWYADALVRLNPGVGSVLLLTGIGGGAFHHMSQVSFEGGGSP
                     LGRGISGATYIPNERIFLGFKASVELATVGSDKIFNGDATFEIAFTNRGGIEYIDFQG
                     NGYFLTAVEVVDEAKMSEKVGKMANSTGSSSAPLKPAPIAAHMRINYDFPNRTLHGNF
                     EVYINVAGGIIKGIGANDRAGWSVIHISPGEWYMYVGTPSDPLGVELLGMVRSESYFM
                     MGQNIPASPPPPEKVSEILGGIDLDYMAELNDLGEGRGIAFGTRLLMDTGEMQFFPFY
                     ASLSMGMGFDLMMKDYGNAECVGHNGPIGISGWYANGQAFAYFEGKIGILIKLLKKTK
                     RYEIINLGAAAVLQAKFPNPSWLYGVVGGYFNILGGMVKGDCRFEVTIGEECELFNRA
                     GVLENIEVIAAITPGEGEAEVSVFNAPQAVFNMAVDHQFQLVDEYDQIRFFKIGLDFA
                     NVKDGETEIEGKVSWNSDHTTLLFESHEILPPTKSLAFSVSVFYEEKINGNWTPVLDN
                     GSKISETRSVTFTTGEAPDYIPENNVLYSYPLRNQYNVYKGESSVGYVQLKQGQDYLF
                     HPGPEWDQKTRFVQVGNANPKLGSVGYDAANKRVSFSFPDMSPNKIYKLEIVNIPTGT
                     VTMDQNVSAVESTFTADENITIETKTAEGTIDILEEKSLFDLAFRTSLYSTFTEKVNA
                     INFGETFMDPNFDYQGVVDFGSLLINNSELFDNYELQGTETIPPLITLEATPDNPWLS
                     GLIPVIYSQYPVGNLFSFTWRSSEPYGVPPLRTMHYSNLNVDALLGENALITGSGGIQ
                     FNPETIIYKVPYFVYKDLGDLQNQIYNYMCTHSATSKMNQIVNTTYPLLNINYSYNYQ
                     FKYHLPGNSNLKSSAPRSLKINFE"
     gene            3860..>4635
                     /locus_tag="VGA21_15165"
     CDS             3860..>4635
                     /locus_tag="VGA21_15165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651899.1"
                     /translation="MRRLISILVFLIPATISLGQGSNTTMIISKHYGDSIVLRWAPLS
                     PAAWNLCNKYGYTLERYTITRDSVLLDTKSRMILTPIPLKPKPLEDWEELIETDPFSA
                     IIAQAIYGENFDVNTGQQSGMAQIMQQVKELERRYSFAVFSADLSAIAANYAGLRWVD
                     HDIRKNETYIYRVIPAVPPAILPSDTAFVYVRNDWKPDLHKPFNVKAEFEERKVTLSW
                     DENVLERKFSAYQVERSDDGGRSFHKISELPIINTYKEEN"
BASE COUNT         1380 a          847 c         1082 g         1326 t
ORIGIN      
        1 agtttgtacc ggagaaaaca gacggtaatc ctgacaccgg taaacaggtc aaagcatcat
       61 ttgatatagt cattgatgac tggaacaata tgctggtgtc gttgaatatt gatccttttc
      121 agttaaaaag catgaatggc ctgggcttca aagtgactaa tgcggttatt gatatgagtg
      181 atacccgtga tcctctaggc gtctcttttc cggcgggata taatgccggt acaccacctg
      241 gcctttggcg cggcgtattt atacgggagt tgagcataaa acttcccaaa caatttaaat
      301 ctaatgacac tcaatcaaat ccgcaaaatc cgcggcgaat cgaaattgag ggttccaata
      361 tgataatcga caaccaggga ttcaccggtg ttatttcagc taattatctc ttaaatcttg
      421 atcagggtgc catcgggaag tgggcattct cattaaatca ccttcatata gcttttgtgg
      481 gaaaccagat cgaaagtgca ggattctcgg gagcaataag aatacctgtc accggagatc
      541 gggatacttt gagttatagt gcgatcattg atccgggtaa ttcctatatt tttacaatta
      601 cacctccgga ttcagtcagg ataccaattt ttaatgccac aaaggtagta ctaagtccgg
      661 cttcttccct cactattgtt attgaagacg gtgagttccg tcccagggca aatcttcatg
      721 gtgtactggg aattggtgga gacaaagttg ctcttgcgca aattgaattt gaaaatcttc
      781 aagtccagac agttagtcca tacctaagta tcggaaactt atcatttggt tctcctgccc
      841 ttgaacaaaa gatggggaat ttccctattc agatccataa tatcggcttt cagaatcggg
      901 agaataataa agtggcatta ctatttgaag tgacagtggc tttaatgaaa ggagaggaca
      961 gtggtggaat tggtggttca gcaggttttg ttattgaagg taaaatcgaa gatggtggca
     1021 acttgctgga tttcaaatat gaccaccttg agctaaataa aatttccatt aatgctgata
     1081 taggagcagt aaaacttgcg ggtacgctgg agtggtttga agaagatcct atttacggca
     1141 aaggagtaaa aggatacgcc atgctggagg ttaccaagat ttctttgcaa gcgacggtat
     1201 tattcggaaa aataaatgga ttccgttatt ggtatgctga tgcattggta agattgaacc
     1261 ctggtgtggg gagcgttttg ttattaaccg gaattggcgg cggggcattt catcacatga
     1321 gccaggtgag ttttgagggt ggtggcagtc cgttgggccg gggtatttcc ggagctacat
     1381 atatccctaa cgaaagaatt tttttgggtt tcaaggcaag cgtcgaactg gcgaccgttg
     1441 gatcggataa aatatttaat ggggatgcca cctttgaaat tgcgtttaca aacagaggcg
     1501 gaattgagta tatagatttt caggggaatg gatattttct aactgctgtg gaggtagttg
     1561 atgaggccaa gatgtctgag aaggtaggta aaatggcaaa ttccacaggc agtagttctg
     1621 cacctttaaa accggctccg attgcagccc acatgcgaat taattatgat tttccaaata
     1681 gaactttgca tggaaatttt gaggtataca ttaatgtcgc cggtggtata ataaagggca
     1741 ttggagcaaa tgatcgtgcc ggctggtcgg taattcatat ttcaccgggt gagtggtata
     1801 tgtacgttgg aactccaagt gacccacttg gtgtagaact actaggtatg gttcgctctg
     1861 aaagttattt tatgatgggg cagaatattc ccgcaagtcc accaccccct gaaaaagttt
     1921 cggaaatact cggcggcatt gatttggatt atatggcaga actcaacgac ctgggcgaag
     1981 ggagaggaat cgcattcgga acacgactgc taatggatac cggcgaaatg cagttctttc
     2041 ctttttatgc atcgctttca atggggatgg gctttgactt gatgatgaaa gattatggca
     2101 atgccgagtg tgtcggtcat aatggcccaa tcggaattag cggttggtat gccaatggcc
     2161 aggcctttgc atattttgaa ggaaaaattg gcatccttat aaaactatta aagaaaacca
     2221 aaaggtacga aataattaat cttggagcag ccgctgtgtt acaggcgaag tttccgaatc
     2281 caagctggtt atatggggta gttggagggt actttaatat tctgggcgga atggtgaagg
     2341 gagattgccg gtttgaggtg actataggag aagagtgtga gttgtttaat agagctggag
     2401 ttctggagaa cattgaggtt atagcagcca tcacaccagg ggaaggggaa gctgaagtca
     2461 gtgttttcaa tgcaccacag gcggtattca atatggctgt agaccatcaa tttcaacttg
     2521 tagatgaata tgatcaaata cggtttttca aaataggtct tgattttgca aatgtaaaag
     2581 atggagaaac agaaattgaa ggtaaggtaa gctggaattc ggatcacaca actttacttt
     2641 ttgaatcgca tgaaattttg ccgccgacta aatcgcttgc gtttagtgtt tctgtttttt
     2701 atgaagagaa aatcaatggt aactggactc ctgtgcttga taatggaagt aaaatttcag
     2761 aaaccagatc ggttactttt actaccggag aagctcccga ttacatcccg gaaaataatg
     2821 tactctatag ctatccattg cgaaaccagt acaatgttta taaaggcgag tcttctgttg
     2881 gatatgttca gctgaaacaa ggccaggatt atttgtttca tccaggcccg gaatgggatc
     2941 aaaaaacgcg gtttgtgcaa gtggggaacg caaatccaaa attgggcagc gtaggctatg
     3001 atgctgccaa taaaagagta agcttttcat ttccggatat gagtccaaat aaaatttaca
     3061 aattagaaat cgtaaatata cctaccggaa cagtcactat ggatcagaac gtttccgccg
     3121 ttgagagtac ttttactgca gacgaaaata ttacaataga aacaaagacc gcggaaggga
     3181 cgatagatat tctggaagaa aaaagtcttt ttgatttagc tttccggaca agcctgtata
     3241 gcacgtttac tgaaaaggtt aatgcgatca attttgggga gacctttatg gaccccaatt
     3301 ttgattatca gggggttgtt gattttgggt cgttattaat taacaattca gaattgtttg
     3361 ataattatga attacaggga acggaaacca ttccaccact tattaccctt gaggcgacgc
     3421 ctgacaatcc atggctttcc ggtttaatac ctgtcatata ttctcagtat ccggtgggaa
     3481 acctgttctc ctttacatgg agaagttcgg agccctatgg agttcctcca ttacggacca
     3541 tgcattactc caacctgaat gtggatgctc ttcttggtga gaacgcattg attacaggtt
     3601 ccggtggtat acaatttaat cctgaaacca tcatatacaa ggttccctat tttgtgtata
     3661 aagatttggg ggatttgcaa aaccaaattt ataattatat gtgtacccat tcggcaactt
     3721 ccaaaatgaa tcaaattgta aatacaacct atccactctt aaatataaac tactcatata
     3781 attaccaatt caaatatcat cttcccggaa attccaattt gaagtcaagt gctcccaggt
     3841 ccttaaaaat aaattttgag tgaggaggct aatttcaata ttggtttttt tgattccggc
     3901 aactattagc cttggtcagg gctcaaatac taccatgata atttcaaaac attatggtga
     3961 ctccattgtt ttacgctggg caccccttag tcctgctgcg tggaatcttt gcaataaata
     4021 tggatatact ctggaacggt acactattac ccgtgacagt gtattgctgg acacaaaatc
     4081 caggatgatc ctgacgccaa ttcctttaaa gccaaagcct ctggaagatt gggaagaatt
     4141 aattgaaaca gatccttttt ctgccattat agcccaggcc atttatggag aaaactttga
     4201 tgtaaataca ggacaacaat ccgggatggc tcaaatcatg caacaagtaa aggaactgga
     4261 gcggcggtat tcttttgctg tatttagcgc cgacttgtcc gcgatagccg ccaattacgc
     4321 cggactccgt tgggtggatc atgacatcag aaaaaacgag acctatatct atcgggtaat
     4381 tcccgcagta cccccggcaa tccttccctc cgacaccgcc tttgtttacg tcagaaatga
     4441 ttggaaacct gatttgcaca agccatttaa tgtaaaagct gagtttgaag aaagaaaagt
     4501 aacacttagt tgggatgaaa atgtgctgga gaggaaattt tccgcttacc aggtagagcg
     4561 atcggatgat ggaggaagaa gttttcataa aatttctgag ttgccgataa taaatactta
     4621 taaagaagaa aataa
//
LOCUS       DASWHX010000248         2985 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_1825312, whole genome shotgun sequence.
ACCESSION   DASWHX010000248 DASWHX010000000
VERSION     DASWHX010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 2985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2985
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1825312"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2206
                     /locus_tag="VGA21_15170"
     CDS             <1..2206
                     /locus_tag="VGA21_15170"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX9651900.1"
                     /translation="PNNTYRIEGKTNSDFWLSNIDAEYGKELNSGAYSQVKNVLRIAP
                     MGNLFFNYGDKRFSENNIYRVEPVSEFFEMFRFNFIEGSEKNVLKDPYSIVMTRSTAR
                     KYFGDEPALGKIINYDSLLLTVKGVVEDLPTNTHLNFDLIYAQPGNDQHYHAFTYLRL
                     TKNSDSKRAEELILNMRDDLDEFHKLTTVKLIGVKDIYFESEAAFGNSGAGDRTQLTG
                     FMIAGFLILLIAILNYINLSLAIYSGKRFEVGIRKILGESKLQFNIIMFLESLLISLA
                     SVFLAVFGIELTLPYFSDFLSLSLENKFLSSPYYWLSGVAFVLAVNLIITVYPSLMLG
                     KANVSRLVKSKETINGKQGLGFLNILIFIQFILFFTLGISAWFVNRQIHYIDNKDLGF
                     STHGVIKIRNAFFLNGVENYNLLKERLLTYSEIESVTHGAMMGDDMTPLAYKPEGQDQ
                     IYENLLSFGVGEDYFDVMNIKIVAGDFRRVMEAAQSGHVVSLVNQSFIDQYNWRENPV
                     GKTLILRPGTENELHRTISAVFRDFHFFSLKEKIGPHIISMRKEPTNININVLVKTNP
                     ANLKRAYEIIETEWYAFAPDRPVEYVLMDDEIRSFYQQERRVGNITLLFSVLALLLSF
                     GGLIIFVTYITKLRSKEIALRQVLGASMMQIILVLNKKTFWIIMLAALFGSTFSYLLI
                     NQWLTNFANTITLHPIAFIAATIAGYGIAFMIIVGQSYKSAISNPLATLGQE"
     gene            2576..>2985
                     /locus_tag="VGA21_15175"
     CDS             2576..>2985
                     /locus_tag="VGA21_15175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9651901.1"
                     /translation="MLKNYLKISFRNIFRHKGYSFINIFGLTVGLTTSIFIFLWVVDE
                     LSYDRFHANADNIYRVMINNTYPDGRIITHGATPVKLKDAMQTGVSEINLITQLSMDT
                     ELLIKHDVKSFNEQGIYADKTLFSVFSFSKIKGNP"
BASE COUNT          889 a          556 c          600 g          940 t
ORIGIN      
        1 tccaaataat acctaccgga ttgaaggaaa gaccaatagc gatttctggt tgtcaaacat
       61 tgatgctgaa tatggaaaag aattaaattc cggggcttat tcacaagtga agaacgtttt
      121 gagaatagcc ccaatgggga accttttttt taattatggt gataagcgtt ttagtgagaa
      181 taatatttat cgtgttgaac cagtatcaga attttttgag atgttcaggt tcaactttat
      241 tgaaggaagt gaaaaaaatg ttctgaagga tccatattca atcgtgatga cccgctccac
      301 cgcgaggaaa tactttggcg atgaacctgc acttggcaag ataattaatt atgattcgct
      361 attgttaacg gtgaaaggtg tggttgaaga cctgccgaca aatacccatc tgaactttga
      421 tttaatttat gcgcaacctg ggaatgatca acactaccat gccttcactt atctgaggct
      481 aaccaaaaac tctgattcaa aacgtgcgga agaattgatc ctgaacatga gagatgattt
      541 ggatgaattc cacaagctca cgacggtaaa acttattgga gtgaaagaca tctacttcga
      601 atcggaggct gcatttggaa atagcggagc cggtgacaga actcagttaa ctgggttcat
      661 gatcgccggt ttccttatcc tgctgattgc catattgaat tatatcaacc tttctctggc
      721 tatttattca ggcaaacgtt ttgaagtcgg cattcgtaag attttgggtg aatctaaact
      781 tcagtttaac atcattatgt ttctggaatc tctcttaatt tcattggcaa gtgttttttt
      841 ggcggtattc ggaatcgaac tgaccttgcc atatttcagt gactttctga gtttgagtct
      901 tgaaaacaaa ttcctctctt ccccttacta ttggctgtca ggcgttgcgt ttgttttagc
      961 agttaatttg atcataacag tttatccatc cctaatgctt ggcaaagcca atgtcagcag
     1021 gttggtgaaa agcaaagaga caatcaatgg taaacaaggt cttggttttt tgaacattct
     1081 tattttcatc cagtttattt tgttttttac acttggaata tcagcctggt ttgttaatcg
     1141 tcagattcat tatattgata acaaagacct ggggttttca acgcacggag taatcaaaat
     1201 caggaatgca ttctttctta atggagttga aaactataat ttattgaagg aacgattatt
     1261 gacttattca gaaattgaaa gcgtaaccca tggggctatg atgggagatg acatgacacc
     1321 gcttgcttat aaacccgaag ggcaagatca gatttatgag aatctcttat cttttggagt
     1381 tggggaggat tatttcgatg tgatgaatat taaaattgtg gcgggagatt ttcgaagagt
     1441 tatggaagcg gcacagtcag gacatgtcgt ttccctggtg aaccaaagtt tcattgatca
     1501 atataattgg cgggagaatc cggtaggaaa aactctcatc ttaagacccg gaaccgaaaa
     1561 cgaacttcat cgcaccattt cagcagtctt ccgtgatttc cacttctttt ccctgaaaga
     1621 gaaaatagga cctcatatca tctcgatgcg aaaagaaccc accaatatca atattaatgt
     1681 attggtaaaa acaaacccag ctaacctgaa gcgagcttat gaaattattg aaaccgaatg
     1741 gtacgcgttc gcaccagaca ggccggttga atacgtgttg atggatgatg agatccgatc
     1801 attttatcag caggaacggc gggttggaaa cattaccctt ttgttttcag tgctggccct
     1861 gctgctttca tttggcggat taatcatttt tgtcacttac atcaccaagc tgagatccaa
     1921 ggagatcgcc ttaaggcagg tgctgggagc ttccatgatg caaatcatct tggtacttaa
     1981 taaaaagact ttttggatta tcatgctggc agctttgttt ggcagcacat ttagttacct
     2041 gctgataaat caatggttaa ctaatttcgc caatacaatt accctgcatc caattgcatt
     2101 tatcgcggca acgattgcag gctatgggat tgctttcatg atcattgtcg gccagtctta
     2161 taagtcagct attagcaatc cgttagcaac attaggccag gaataaactg attttgatct
     2221 ttttttttaa ttcattttgt gcgaaataaa gttatcattt ctgtcaactt tatgcaggac
     2281 gagtcaatga gccaaatccc agaattcttt aaaacaaatc tttcaatcat cccttttata
     2341 gctccatccc aaagccgacc actatcgtcc gcatgagaac aaacgaggtt tttttgatcc
     2401 ctttaaatca ggcaaattga agattatata atggcatagt gttggtttat gagctcatgg
     2461 tcagataaca gtttattgaa acaaacccgg ttgagtcacc agaaatgagt gataaaatcc
     2521 cgaacttgtc gggtggattt tgttaacaaa tgattaacct tttacctctt aactaatgtt
     2581 aaagaactat ctgaaaattt cttttcgcaa tatcttccgc cacaaaggct attctttcat
     2641 taatatattt ggtcttactg taggactgac caccagtatt tttatttttc tctgggtggt
     2701 tgatgaattg tcatacgatc ggtttcatgc gaacgccgat aatatttaca gggtaatgat
     2761 caacaatact tacccggatg gtagaattat aacacacggt gccacacctg ttaaactgaa
     2821 ggacgccatg cagaccggag tttcagagat taatctcatt acgcaactca gtatggatac
     2881 tgaattatta attaaacacg atgtcaaatc ttttaatgag cagggtattt atgctgacaa
     2941 aaccttgttt tcagtattta gcttttcaaa aattaaaggc aatcc
//
LOCUS       DASWHX010000249         2777 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_3282791, whole genome shotgun sequence.
ACCESSION   DASWHX010000249 DASWHX010000000
VERSION     DASWHX010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 2777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2777
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3282791"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="VGA21_15180"
     CDS             complement(<1..266)
                     /locus_tag="VGA21_15180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651902.1"
                     /translation="MADIVPKDQSINFEDFKNENGLIYWWASEFMVMLGYDDIHSFKK
                     AIDKAMKACISLGIDCLDNFIKEQNNGHEDYKMTRFACYLTAMNA"
     gene            870..1193
                     /locus_tag="VGA21_15185"
     CDS             870..1193
                     /locus_tag="VGA21_15185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651903.1"
                     /translation="MENFKMYLFVTLLIIPFQVSAQDSLKMVSEIRLDLEKFAIQRES
                     GKIIQLVATLGVGITGYLISDGKDVPTAVPIILSGVALVGFIIDMDAGNRLVRKKQRK
                     VKNRN"
     gene            complement(1302..2162)
                     /locus_tag="VGA21_15190"
     CDS             complement(1302..2162)
                     /locus_tag="VGA21_15190"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HEX9651904.1"
                     /translation="MNNCSNIRIGIFALILLISSPIIGQEKLSAILAPRELHDLISKE
                     NIVIVDLRGESDYKDEHIPGSINSNRKLFENTELPYSGMMASKDKVEKILSGLGILPA
                     SQIIIYDESTSLDAARLWFVLKTYGHQKVQLLDGGIQLWKRQNFVVTKDTGRVNPSSY
                     KFPEQKGLEYYATREEVKSVLKNQNVVILDVRSEREFSAGHIPGAIHLEWTNNLEKEN
                     VFKSSEALKEQFEKLGVTPDKQIITYCRSGVRAAHTGFILKDLLGYENVKIFDGSWIE
                     WLHFGEEVEK"
     gene            complement(2217..>2777)
                     /locus_tag="VGA21_15195"
     CDS             complement(2217..>2777)
                     /locus_tag="VGA21_15195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M1"
                     /protein_id="HEX9651905.1"
                     /translation="LMKSWWNDSKKIPGLTLSENDYTQLAYELAVRGADDSEIILKTQ
                     SGNIKNPDRKQRMEFVIPALSSNKEVRDQFFESLKMEINREHEPWVLEALGYLHHPVR
                     SAHAVEYIQGSLELLEEIQKTGDIFFPTRWLGATFSGHNTKEAAEIVRRFLDSHPHYP
                     SKLREKILQTSDPLFRASVWNKESIE"
BASE COUNT          856 a          551 c          472 g          898 t
ORIGIN      
        1 gcattcatcg cagttagata acatgcaaat cgtgtcattt tgtaatcctc atgcccatta
       61 ttttgttcct taatgaaatt atccagacaa tcaattccta atgaaataca agccttcatt
      121 gccttgtcta tggccttctt aaaagagtgt atatcatcat agccaagcat caccatgaac
      181 tcagaagccc accaataaat taggccattt tcgtttttaa aatcctcgaa attgattgat
      241 tgatccttcg gtacaatatc agccatcgta gcattatttt gtatagggca aatttacaag
      301 aatgtgtaag catttctaag gattacttgg ctaaatcttt atatttcaac cttcctgctg
      361 tatttctgga ctgacacttc aaacctttca tttactgcca atcctctcct atttacaatt
      421 ggattaacct gcaagaaata cgcatacttg gtatacacgg cagaaatgac tgttaagaat
      481 attgaaattc tgagaaagca acttgaacta attgaattga ctatcactta aaaaaatagg
      541 ggaacttttg gttcccttat gtgttggaag gttgttaaac ttatttcaac attgatggaa
      601 aaacggcagc atcagatgtg ccgtgaacct cataaccccg ccccctcatt aggagtctcc
      661 cattaggaaa agagagcaaa aaaatatgaa caaaaaatag cgttggaaaa tgaagttaat
      721 tttgaagacc tcattaaatc agggctgaat tatgatccaa aggaaaaacc caaaaaagcc
      781 aaaaagaaga agcaatctaa aaaatgagga tttatcttta caaaatacag aaggtagatt
      841 aacctacgac caactaacta aaaaatagta tggagaattt caaaatgtat ctatttgtaa
      901 cacttcttat tatacctttt caagtttcgg ctcaagatag tcttaaaatg gtatcagaaa
      961 taagacttga tttggaaaag tttgccattc aacgggaatc tgggaaaata atacaattag
     1021 tagcaacttt gggtgttgga attactggct acttgataag cgatggtaaa gacgtgccca
     1081 ctgctgttcc aataatactc tcaggagttg cacttgttgg atttatcatt gatatggacg
     1141 ctggtaatag attagttaga aaaaaacaga gaaaagtaaa aaatcgaaat taatgccttt
     1201 gtcccaagtt gtatgtagtt gccaatattt ataatggggt tgtttgctca aagaagttaa
     1261 tcttttttta gcaataatcc cctttagggc gaaaagtcac actatttctc cacttcttcg
     1321 ccaaagtgta accattcaat ccatgatcca tcaaagattt taacattctc atatcccagc
     1381 aaatctttta aaataaatcc ggtatgagca gcgcgaactc ctgaacggca ataagttatg
     1441 atttgtttgt ctggagtcac tcccaacttt tcaaattgtt ctttcaatgc ttcactggat
     1501 ttaaatacat tttccttttc cagattattc gtccattcta aatgtattgc acccgggata
     1561 tgaccggcac tgaattcacg ctctgaacgt acatcgagaa taacaacatt ttgatttttc
     1621 aaaacactct ttacttcttc ccttgttgca taatattcca atcctttttg ctcagggaat
     1681 ttataggatg atggatttac tctgcctgta tcctttgtaa caacaaaatt ttgccttttc
     1741 cataactgta tcccgccatc cagcaactgc actttctggt ggccgtaggt tttcaaaacg
     1801 aaccataacc gcgcagcatc caagcttgtg gactcatcat agataataat ttgtgaagct
     1861 ggaagtattc ccaaaccact caatattttt tcaactttgt ctttggatgc catcatacct
     1921 gaatatggta attcggtatt ttcaaatagt tttcgatttg aattaattga tccgggaata
     1981 tgctcatctt tatagtcact ctcgccacgc aggtcaacga ttacaatatt ttcctttgaa
     2041 attaaatcat gcagttctct gggagcaaga attgcactaa gtttttcttg cccaattatt
     2101 ggtgagctaa tcaacaggat taaagcaaaa atgccgattc ttatgtttga gcagttgttc
     2161 atcaatttga ttattagaat tctttagtta aatctacaat ttaaaatgca acagtttcac
     2221 tcaatacttt ccttattcca tactgaagca cgaaataatg ggtctgaagt ctgtaaaatc
     2281 ttttctctga gtttacttgg ataatggggg tgagaatcca ggaatcttcg aacaatttct
     2341 gctgcttctt tcgtattatg accggaaaaa gtagctccaa gccagcgggt agggaagaaa
     2401 atgtctccgg tcttttgaat ttcctccagc aattccagac ttccctgtat atattcaaca
     2461 gcgtgagcag acctcaccgg atggtgcaaa tatcccaatg cttccagtac ccagggttca
     2521 tgctcccggt tgatttccat tttcaggctt tcaaaaaatt gatcccggac ttctttattg
     2581 gaagataatg ccggtatcac aaattccatc cgttgttttc tgtcaggatt ttttatattt
     2641 cctgactggg tttttaaaat tatttctgaa tcatccgctc cccgcaccgc cagctcgtat
     2701 gccagttgtg tgtaatcatt ttctgagagg gttaaacccg gaatcttttt ggagtcgttc
     2761 caccaggatt tcatcaa
//
LOCUS       DASWHX010000250         8720 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_2925590, whole genome shotgun sequence.
ACCESSION   DASWHX010000250 DASWHX010000000
VERSION     DASWHX010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 8720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8720
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2925590"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(64..1602)
                     /locus_tag="VGA21_15200"
     CDS             complement(64..1602)
                     /locus_tag="VGA21_15200"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator"
                     /protein_id="HEX9651906.1"
                     /translation="MKQVPLLIYLLMLCLTSYSQSSNSKVEDALKKAFEAQNPEDGLV
                     FLNKVGELINSADDSTKLEYYIAKGVAHGQLGNADSSFYFLDQAESISKQLTNDLQLI
                     KIYNTEGLVLMGLSKYEEALDAFQKGLAIGENKKDKKYLVAVRKILGNSGGIFYQLGD
                     YEGALRHTRQALKISEQLNDSSGLAYNNLRLALTYQAMDSLITCIEYLKVSSNLLQHL
                     KDTTTMLYVENTLGLVYNKRLQFENSLQHYQEAFKLASLIKNQEEVIHTKIFIAKNYL
                     QLDKLAFTEALALEVIDETIRNGMAKHRQSAYDLLYEIAVKRNDWQLALNYRNKSLDI
                     KDSIANVEIKSRIAELETKYETQKKEAEIVRLSLENDLNVANLAISRNANIAMAVGGL
                     LTVILLIVFFTLRHKKQLAEKEAQDLQIEALKKRFMELHSSPAELAVALEFDELNNKL
                     NTHLTGREFDALKLSLGGKTNVEIADALFISVSTVKFHLRNTYSKMGVGNRKEAFQLM
                     LKSS"
     gene            complement(1935..2906)
                     /locus_tag="VGA21_15205"
     CDS             complement(1935..2906)
                     /locus_tag="VGA21_15205"
                     /inference="COORDINATES: protein motif:HMM:NF042645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6600 domain-containing protein"
                     /protein_id="HEX9651907.1"
                     /translation="MKSFTQGLAMLILLNTTACIASYSTPNAPGPRPMGYGTSPQQVQ
                     ISLQVFYDQLSPYGTWVSYNNYGYVWIPSAGPDFFPYASEGHWVFTNIGWTWYSNYSW
                     GWAPFHYGRWLFDNYYGWIWVPDTEWGPAWVVWRGGGDFYGWAPLEPGINISIVLGGN
                     HQVHHDRWIFVRNGDIGRSNVYSYTVNRTSNTTIINNTTVIQNTRTNTQNNKTYIAGP
                     DRNDVQRVTGRPVRQVAIQEATQPGQMLKGDQLRIYKPAVQRNGNSGQKSAPAKIENL
                     KNVKPVNERPKVNSQQGKQSPAKGKGNQEKPAKKKGKKGGNKGHEYS"
     gene            3225..3557
                     /locus_tag="VGA21_15210"
     CDS             3225..3557
                     /locus_tag="VGA21_15210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651908.1"
                     /translation="MSILLFSKRKNTAAQLLAFLVLVLIWHHLQSISIYLGFIKVFPH
                     FYGADLGSLFLIGPLFLFLFYVRYQMKEKTRPLKLNDWIHFLPFAIAIIVHPVIFQGT
                     ATSEIVKQ"
     gene            3783..4400
                     /locus_tag="VGA21_15215"
     CDS             3783..4400
                     /locus_tag="VGA21_15215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008627808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651909.1"
                     /translation="MSKDKYIQDLREIKEIMSRSSRFISLSGLSGVSAGVFALMGAYM
                     AYKTVYTNNDYSTYGQAILTSENLTTLLLIAMVTLVLSIGIGIYFTTKETKKQNQKIW
                     DHQTKRLLINLAIPLITGGILCLMLLLRGYVGIVAPLTLIFYGLALVNASKYTLSEIR
                     SLGLAEIVLGLISMQFIGFGLLFWSVGFGVLHIVYGAAMQMRYKS"
     gene            4397..4687
                     /locus_tag="VGA21_15220"
     CDS             4397..4687
                     /locus_tag="VGA21_15220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007660395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEX9651910.1"
                     /translation="MKKLLRDLDKAFENKIRLGIMSALIVNEYLDFNSLKDLLDVTDG
                     NLASHLKLLENNKYVKFRKEFLNRKPNTKYSLTSKGREAFMKHIRAIEQLLN"
     gene            4742..6082
                     /gene="creD"
                     /locus_tag="VGA21_15225"
     CDS             4742..6082
                     /gene="creD"
                     /locus_tag="VGA21_15225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015265266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HEX9651911.1"
                     /translation="MDTSETFIDKLSNWIKNSVILKMTTIAIMVLLLLIPTSMIESVI
                     SEREQLRAQATNEVSSKWAGSQQIMGPILTIPLTYEYVQDEKLVSLVKLWYLLPMELR
                     IKGELNPRKLRRGIYEVVVYESNLVISGSFDKIQMPNQTNLKEIAYDQAFLTIGISDL
                     KGIKKPINIKLNEKTLTVEPGSRISDLVYSGVTVDFPDIENLFKQNADFQFSLDLQGS
                     KDLSFIPIGSTTEVEIASSWESPSFSGNFLPDSREVTTDGFKASWLILQLNRNFPQSW
                     IGDDLSKKMEAASFGVDLILPLDDYQKSMRSVKYAIMTITLTFLIFFLVEILNKRKIH
                     PFQYTLVGLALCLFYVLLVSISEHSNFNFAYGISTLGIVSMVALYSLSIFKRRKLTVI
                     LVTTLTGIYGFLFVTLQLSEYALLMGSLGLALILGATMYFTRNINWYKLNIETE"
     gene            complement(6309..6863)
                     /locus_tag="VGA21_15230"
     CDS             complement(6309..6863)
                     /locus_tag="VGA21_15230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018344240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEX9651912.1"
                     /translation="METTAVATKTKWVLDPAHSELLFRVKHLMITTVKGEFTKFSAEV
                     ESKGNDFSKATIRVTVDTNSVFTNEDTRDNHLRSADFFDAEKYRELTFEGTSFEKVND
                     ENYQLKGSLTIKGVSKQIQLDVEFGGINKDPWGNEKAGFSLSGKINRKDFGLNWNTAL
                     ETGGVLVGDEVKLAGEIQLVKQTD"
     gene            complement(6970..7551)
                     /locus_tag="VGA21_15235"
     CDS             complement(6970..7551)
                     /locus_tag="VGA21_15235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020606448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX9651913.1"
                     /translation="MYEQLLKTVQQKVTLTSDEAELLKQFFTLKKVRKRQYMLNEGEV
                     CKYNLFVAKGLLRAFGVEENGYEQVVQFAVEGWWISDLNSFFSGDVAVYNIEALEDCE
                     LLLLTRQSMDEMLEKLPKMERYFRLLMQNHIVALRQRIIASQRHSAEERYIRLIEGFP
                     TILARVPQQYIASYLGMTPETLSRVRKQISEQK"
     gene            7706..7888
                     /locus_tag="VGA21_15240"
     CDS             7706..7888
                     /locus_tag="VGA21_15240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651914.1"
                     /translation="MIEDGFRIEKLLFSFERAGFNDEGEEPKALVLKMLESASFEVEM
                     GTGKLVLSGYKNFMGI"
     gene            7932..>8720
                     /locus_tag="VGA21_15245"
     CDS             7932..>8720
                     /locus_tag="VGA21_15245"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX9651915.1"
                     /translation="MERRIFVTGATGFIGMKLVSRLVESGNIVHALVRNPAHNVLASN
                     SSVKLVHGDLMNIRAIYKGMENCSQVYHLAGNVSTWAKDPKDFYETHVAGTQNILEAA
                     RKWNIEKIIITSSGAVLGPSTSNPVTENSKRVLPFFNDYEKSKHLMERLAKRYSTGRL
                     QIVLVNPARVYGPGLIRESNTAIEIIRKYLQGNWKLIPGRDHIGSYAYVDDVVDGHIL
                     AMEKGRSGERYILGGDNRKFSDLFVITRNLTDVNRKLYKIPLSLV"
BASE COUNT         2513 a         1621 c         1806 g         2780 t
ORIGIN      
        1 agacttgctt ttacctgacc ctatgggtta gttttctaaa aataaattat aactaattga
       61 tattcaactt gatttaagca taagttgaaa tgcctctttt cgattgccca cacccatttt
      121 ggagtaggta tttcttaggt gaaatttaac cgtactcact gaaatgaaga gtgcatcagc
      181 aatttcaaca ttggttttac cccccaggct caatttaagc gcatcgaatt cgcgccctgt
      241 aaggtgagtg ttcaacttat tgttaagttc atcaaactcc aaagccacag ccagctctgc
      301 aggacttgaa tgcaattcca taaagcgctt tttcaatgct tctatttgta ggtcttgcgc
      361 ttctttttca gcaagctgct ttttatgcct tagtgtgaaa aaaacaatca gcaaaattac
      421 cgtgagcaaa ccacccaccg ccattgctat attagcattt ctggatatgg ccaggttggc
      481 aacattcaga tcattttcaa gggatagcct tactatttca gcttctttct tttgtgtttc
      541 atatttagtt tctaactcgg ctattcgaga ctttatttcc acattcgcta tggagtcttt
      601 tatatccaaa cttttatttc tgtagtttaa ggctagctgc caatcatttc tttttacagc
      661 tatttcataa agtaaatcat aggccgattg tcgatgcttc gccattccgt tacggatcgt
      721 ttcatctatc acctccaagg caagggcctc tgtaaatgct aatttatcaa gctgtaaata
      781 attttttgca ataaagattt tagtgtgaat aacttcttcc tggtttttta tcaatgatgc
      841 cagcttaaaa gcttcctggt aatgctgtaa tgaattttca aattgtaatc ttttgttata
      901 tactaaaccc aatgtatttt ccacataaag catagtggtg gtgtctttca aatgctgcag
      961 taaattgctg gagactttta aatattcaat gcatgtaatt aaactatcca ttgcctgata
     1021 ggtgagtgcc agtctaaggt tattgtaagc taaacccgaa gaatcattga gctgctctga
     1081 tatcttcagg gcttgtcgag tgtggcgcaa ggcaccttca taatctccca actgataaaa
     1141 aatgccacct gaattaccaa gaattttacg aaccgctacg agatacttct tatctttttt
     1201 gttttctcca attgccaatc ctttttgaaa tgcatcgaga gcctcttcgt acttactcag
     1261 gcccatcaga accagtcctt cagtgttgta gattttaata agttgcaaat cattggtcaa
     1321 ttgcttacta atgctctcgg cctgatctaa gaaatagaaa gacgaatctg cattgcccaa
     1381 ctgaccatgg gcaacacctt tggcaatata atactctagt tttgttgaat catccgcgct
     1441 gtttattaat tcaccaacct tatttagaaa tacgagccca tcttcaggat tttgtgcttc
     1501 aaaagctttt tttagtgcat cttcaacttt ggaattgcta ctttgggaat agctggttaa
     1561 acacaacatt aacaggtaaa tcaaaagagg tacttgtttc aaaattggct tgttgattgt
     1621 tgtaaatgct aatataatag ggtttaggta gtattgaaat tgaatgaggt agtattaata
     1681 tgtccataag agaaaacagc ctgaattctt atccaatcgc atgccggaag cagtgaagca
     1741 gtgaagcagg gaagcaggga agcagtgaag cagtgaagca gtgaggcagt gaagcaggga
     1801 agcagtgagg cagtgaagca gtgagcagtg agcagtgaaa cagaaggagg gtcagggtgg
     1861 ttttacgcac ccactaagga acgggtatcg ttgcttgtct gtatcaacta agcactggca
     1921 ttttaaactc ttaactaaga gtactcatgc cctttatttc cccctttttt cccctttttc
     1981 ttggcaggtt tctcttgatt acccttgcct ttggctggag attgctttcc ctgctgcgaa
     2041 ttaactttcg gtcgttcatt cacaggcttt acatttttca aattttcaat tttagccggg
     2101 gctgattttt gtccggaatt cccattacgc tgtacagcag gtttataaat tctcagttga
     2161 tctcccttta gcatttggcc aggttgagtt gcttcctgaa tagcgacttg tcttaccggt
     2221 ctgccggtta cacgctgaac atcattcctg tcaggacccg ctatgtaagt tttattattt
     2281 tgtgtgttag ttctggtatt ctgtataact gttgtgttat tgataatggt ggtattgctg
     2341 gtgcgattaa cggtgtagga atacacattc gaccttccta tgtccccgtt acgaacaaag
     2401 atccagcgat catgatgcac ctgatgattt ccgcctaaca ctatacttat attgatgcct
     2461 ggttcgaggg gtgcccatcc gtaaaaatcg cctccgcctc tccataccac ccaagccggg
     2521 ccccattctg tatccggtac ccatatccat ccgtaataat tatcaaacaa ccatcggccg
     2581 tagtgaaagg gcgcccatcc ccaactataa ttagaatacc atgtccaacc tatattggta
     2641 aacacccaat gaccttccga agcatagggg aaaaaatccg gtccggcact aggtatccaa
     2701 acatatccgt aattattata tgatacccaa gtaccatagg gacttaattg atcatagaaa
     2761 acttgtaaac ttatttgtac ttgttgtgga cttgttccat agcccatggg tcgtgggcca
     2821 ggtgcgtttg gtgtcgagta ggatgctatg caagctgtcg tatttagtag aatcaacatt
     2881 gccaaccctt gtgtgaagct tttcattggt gtatcggttt aatatatata ggttatagtc
     2941 tttgaaaatt tccagtttgg aataaacggt ttaaaatttt cgatatgacc ttacaaagtt
     3001 gagttctttg ttattgattt atgttacaca ttagtgcaaa tgacttacat atttcataca
     3061 tttgttgttg tgatggtttc catgaatgcc attttaataa gcatttacta caattaacct
     3121 atttaatacg tatacttaag gaaagtatat tcggtgataa gagaaagcat atttgactgg
     3181 tggtccctgg taattatgct gggattgtac agggtttgat agtgatgagt attttgcttt
     3241 tcagcaaaag aaaaaataca gccgcacaat tacttgcttt tttagtcctc gtcctgattt
     3301 ggcatcattt gcaaagtatc tccatttacc ttggctttat taaagtattc ccgcatttct
     3361 atggggcgga tcttgggtct ctgtttctta ttggcccgtt gttcttgttc ttgttctatg
     3421 tacggtatca aatgaaggaa aaaaccagac ctttaaaatt gaatgactgg atacatttcc
     3481 tgccctttgc aattgcaata attgttcatc cggtaatttt tcagggaacg gcgaccagtg
     3541 aaatagtgaa gcagtgaaac agtgaaacag tgaagcagtg aagcagtgaa gcagtgaaac
     3601 agtgaaaaag tgaatcagtg aaacagtgaa acagtgaagc agtgaagcag tgaagcagtg
     3661 aagcagtgaa gcagtgagta tttagcaatg aacagtgagg caaggatttt ctcctgtttt
     3721 tttagcagtt atcttgcaaa gtaacaaatt taccttttac tttgtatttc aaagtacttt
     3781 ttatgagtaa agataaatat atccaggacc taagggaaat caaggaaata atgagccggt
     3841 cgtcccggtt tatttcgctg agtgggcttt cgggggtttc agccggggtt tttgcattaa
     3901 tgggggctta tatggcctat aagacggttt acaccaacaa tgattattca acatacggac
     3961 aagccatctt gacttcggaa aacctgacaa cgctactttt gattgctatg gtgacacttg
     4021 ttctttctat tggtataggg atttatttta caacgaaaga aaccaagaag caaaatcaga
     4081 aaatctggga tcatcaaaca aagagacttt taatcaacct tgcaattcct ctcattacag
     4141 gtggcatact ttgtcttatg ctactcttaa gaggttatgt cggaatcgta gctccgttaa
     4201 ccctgatatt ctatggtttg gcgctggtaa atgcaagtaa atatactctt agtgaaatcc
     4261 gaagcttggg actagctgaa attgtgttgg gattaatatc catgcaattt attggctttg
     4321 gattattgtt ttggtcagtt gggtttggtg tactgcatat cgtgtatggg gcggccatgc
     4381 aaatgaggta taaatcgtga agaaactgtt aagagatctt gataaggcgt ttgaaaataa
     4441 gattcgactt ggcataatgt cagcattgat tgtgaacgaa tatcttgatt ttaattcatt
     4501 aaaggatttg ctggatgtga ctgatggcaa cctggccagt caccttaaac tcctggagaa
     4561 taacaaatat gtaaaattta gaaaggaatt cctgaaccga aagcctaata ccaaatattc
     4621 tttgacgagc aaaggaaggg aggcattcat gaaacacatc agggccattg aacaacttct
     4681 taattaaaaa atttttaact ataaactttg tattgcaaag tactttcaat aaataatatt
     4741 tatggataca tctgaaacat tcatcgacaa attaagtaac tggattaaga attcagtaat
     4801 acttaagatg acaactattg ccattatggt gttgttatta ttgataccga catccatgat
     4861 tgagtctgtt attagtgaac gggaacaact aagggctcag gcaacgaacg aggtcagttc
     4921 caagtgggcc ggaagccagc aaataatggg gcccatttta acaataccat tgacatatga
     4981 gtatgtgcag gatgaaaagt tggttagctt ggttaagctt tggtatttat tgcctatgga
     5041 attaagaatt aaaggagagt taaatccccg gaaattaaga agaggtattt acgaggtagt
     5101 agtgtacgaa tcaaatcttg tgatatccgg gagctttgat aaaatccaga tgccgaacca
     5161 aacaaatctt aaagagatag catatgatca agcatttctt accatcggaa tctctgattt
     5221 gaaaggaata aagaagccaa ttaatatcaa attgaatgaa aaaactctta ccgttgaacc
     5281 aggttccagg atatccgact tagtttattc cggagttact gtggattttc cggatattga
     5341 aaacctgttt aagcaaaatg cggattttca attttccttg gacctgcaag ggagtaaaga
     5401 cctttcattt attcccattg gaagtaccac agaagttgaa attgccagca gttgggaatc
     5461 gccaagtttt agtggcaact ttttacctga ctcccgggag gtgactactg atggttttaa
     5521 agccagctgg ctgattctcc aactgaatcg taattttccg cagtcatgga ttggagatga
     5581 tctatctaaa aaaatggagg ccgcctcatt cggcgttgat ctaattcttc cactggatga
     5641 ctatcagaaa tcaatgaggt cggtgaaata tgctataatg accatcaccc tcacatttct
     5701 gattttcttt ctggtggaga tattgaataa aaggaaaatc catccatttc aatatacact
     5761 tgttggtctt gctttgtgtt tgttttatgt tttactggtt tcgatatcgg agcactcgaa
     5821 ctttaatttt gcatatggta tttctacttt gggaattgta tcaatggttg ccctttattc
     5881 tttaagtatt tttaagagac gaaaattaac tgtaattctg gttacaactt taaccggaat
     5941 ttacgggttc ctgtttgtta cccttcaatt atcagaatat gctttgctca tgggcagcct
     6001 ggggctagca ttgatcttag gcgcaacgat gtatttcaca aggaacatta attggtataa
     6061 attgaatata gagacggaat aagaatttcc agaacgcggg gtttaaagag atcccgcgct
     6121 gtgacgcaag ccccatggaa agcgatataa ttgagctcag accagcgagt ccggagcggc
     6181 ccatgccccg cccaaggcgg ggacacgcct caaattaatt attgcacaaa taatagttta
     6241 actaccagac aaaaaaagtg agcgcttttt aacgcccacc taaactaaaa tttcttaaga
     6301 tttcataatt agtcggtctg cttaacaagc tgaatctcgc cggctagttt cacttcatcc
     6361 ccaaccaaca ctccacccgt ttccaatgcg gtattccagt taaggccaaa atctttccgg
     6421 tttatttttc cgcttagcga aaagccggct ttttcatttc cccaaggatc tttattgatg
     6481 ccaccaaatt caacatccaa ttgtatttgc ttgcttactc ctttaatggt gagactgcct
     6541 ttcagttggt aattttcgtc attaactttt tcgaaggagg ttccttcaaa agtgagttcg
     6601 cgatattttt ccgcatcgaa gaaatcagca cttcgcaggt gattgtcgcg agtgtcttcg
     6661 ttcgtgaata cagagtttgt atctactgtt accctgatgg tggccttgga gaaatcgttc
     6721 ccttttgatt caacctccgc gctgaatttt gtgaactcac ctttcacggt ggtgatcatc
     6781 aggtgtttta ccctgaaaag aagttcactg tgggcgggat ccaaaaccca cttggttttt
     6841 gttgctactg ctgttgtttc cataattgta tttttctgat tttgttctac tctttatgca
     6901 aaactagtga gcttattcaa gagtaaaatt gatgtatgat aagaaaggac ttgatatgga
     6961 tcaagtgcgc tacttctgct cagagatttg tttgcggacg cgactcaggg tttcgggtgt
     7021 cattccgaga taggatgcga tatattgttg tggaacccgg gcaagaatgg tggggaagcc
     7081 ttcaattaac ctgatgtatc tttcttcagc ggagtgcctt tgtgaagcga tgattctttg
     7141 tcgcaacgcc acgatatgat tttgcattag cagcctgaaa tatcgctcca ttttgggaag
     7201 cttttctaac atttcatcca tggattgcct ggtaagtaaa agcagttcac agtcttccag
     7261 cgcttcaata ttatacaccg ctacatcgcc tgagaaaaaa ctattcaaat ctgaaatcca
     7321 ccaaccttcg acagcaaact gaactacttg ctcataacca ttttcttcca caccgaatgc
     7381 acgaagaagt ccctttgcaa cgaagaggtt atatttgcaa acttcacctt catttaacat
     7441 gtactgccgc ttccggactt tctttagtgt aaagaattgc tttaggagtt cagcttcatc
     7501 gcttgtaagc gtcacttttt gttgaacggt ttttagaagt tgttcgtaca caggatgttg
     7561 tttaccattc aaatatagcc agtattaaaa taattctgaa ccaggattta tggtttggtt
     7621 tgaaaggaag cctttaatta atgccggaat aatattttct taattcagta attttgataa
     7681 ttctaattct ggctactttt aacaattgat tgaagacggc tttagaattg aaaaattatt
     7741 gttcagtttt gaaagagctg gctttaatga tgaaggggaa gaaccgaaag cgttggtttt
     7801 gaagatgctg gaaagtgcat cttttgaggt cgaaatgggc accggtaaat tggttttatc
     7861 cggatataaa aattttatgg gcatctaatc tatttcttta agaaagataa ttttaaattc
     7921 attaccagaa tatggagagg cgaatatttg taacaggggc gaccggcttt attggaatga
     7981 agctggttag cagacttgta gaaagtggta atattgtaca tgcgttggtt aggaacccag
     8041 cccataatgt tttagcctca aattcatcgg tgaagttagt gcacggagat ttaatgaata
     8101 ttagagcgat ctataaaggc atggaaaatt gttcacaggt ttatcaccta gcgggtaacg
     8161 taagtacctg ggcaaaagat ccaaaggatt tttatgaaac gcatgttgca gggacacaga
     8221 atattcttga ggccgcaaga aagtggaata tagaaaagat tattattacc agctctggtg
     8281 cagtgctggg tccctccact tctaatcccg ttacagagaa ttcaaaaagg gttttgcctt
     8341 tttttaatga ctatgaaaag tcaaagcatt taatggaacg tttggctaaa cgatattcga
     8401 ccgggagact tcagatagtg ctggtaaatc ctgcgagggt ttatggtccg ggattgatta
     8461 gagaaagtaa cacagctata gaaataatca ggaaatatct tcagggaaat tggaaactga
     8521 ttccaggaag ggatcatatt gggtcttatg cgtatgtaga tgacgtagtg gacgggcata
     8581 tcctggccat ggaaaaaggg agaagtggtg aacgctatat tttaggagga gacaaccgca
     8641 aattttccga tctatttgtg attaccagaa acttaaccga tgttaaccgg aagttgtaca
     8701 aaattccgtt aagcctggtg
//
LOCUS       DASWHX010000251         3522 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_1085799, whole genome shotgun sequence.
ACCESSION   DASWHX010000251 DASWHX010000000
VERSION     DASWHX010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 3522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3522
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1085799"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2250
                     /locus_tag="VGA21_15250"
     CDS             <1..2250
                     /locus_tag="VGA21_15250"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEX9651916.1"
                     /translation="TYTHTVAESSGTVDVVLEATDGTETVSKGFTYVLRTPSEVASRP
                     PGTRRGINYFADDETRAILCLEAPTNLSGYVIGDFTDWKIDSNFKLKQDGDFFWIEIT
                     GLTPGTEYAFRYLIDETIYIADPYSDKILDPDDRFIDENIYPGLKTFPEAALVPQWYF
                     NRLSILQTGQEYFFWQNDNYQKPEKEKLVIYELLIRDFFGPGQESFQNLIDTLSYFKS
                     LGINTIELMPVTEFNGNDSWGYNNTFMFAPDKAYGPKNKLKEFIDAAHSKGIAVVLDV
                     VMNHHDIPSPLLLLDFDYTTFKPGSTNPWFNVEAPHSVIKFFFDIKHSSVYTQSYLDS
                     LNHYWINEYHFDGFRFDFSKGFTQKFTSSFESLAQYDPQRVSILKRMANKIWSYSPDT
                     YVILEHFADNTEETDLANSGMLLWGNSQYAYKQNALGFVEDSDIDWIFHRTRGWNNAH
                     LVGYVESHDEQRLMFENLNYGNQSGFYDARNLHTALNRMKAIAATFFSIPGPKMIWQF
                     QELGYDIDINQNGRVGRKPVPWNDAEGLDYNNEEARVSLRNNFAEMIKLKNAYSIFST
                     ADVTFLGENTLIRQIILKNDPYTKTPANHEEMNVVVISNFDIISKNISVNFPHTGTWY
                     NYFANLDEVQVESLPHTVELNASDFRIFTDYRLPKPDLITSVNDSPLTPGIHIYPNPV
                     NETMTIDTGEFIPFQIRIISLSGQILLNSTLDSAQKHSVDVSFLPNGMHILVMESAGV
                     NYVTKIIKQ"
     gene            2251..3252
                     /gene="mgrA"
                     /locus_tag="VGA21_15255"
     CDS             2251..3252
                     /gene="mgrA"
                     /locus_tag="VGA21_15255"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010853628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HEX9651917.1"
                     /translation="MTTVYTPSATRYDTMKYRRCGKSGIQLPVISLGLWHNFGDVDNQ
                     EICRSIIHRSFDLGITHFDLANNYGPPPGSAETNFGNILKKDLSAYRDEMIISTKAGY
                     YMWEGPYGEWGSKKYLVSSLDQSLKRLGIDYVDIFYSHRYDPRTPLEETMEALYLSVI
                     QGKALYVGISNYSPEETKLASGILKTMGIKYLIHQPKYSMFYRWADDTKLFDVLETEG
                     IGAIAFSPLAQGLLTNKYISGIPVGSRASKSHGFLKEKDISEEKIRKVIELNVIAGER
                     GQSLAQMALAWTLRDSRVTSALIGVSSVTQLEDNCATIQNLEFTEQELLRIEEVLKD"
     gene            complement(3253..>3522)
                     /locus_tag="VGA21_15260"
     CDS             complement(3253..>3522)
                     /locus_tag="VGA21_15260"
                     /inference="COORDINATES: protein motif:HMM:NF019365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015926 - glucosidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HEX9651918.1"
                     /translation="ANNILGTDALLAEYPATPTGQRNYIIDLTRIVLESGGQGVIYWE
                     PAWISTSCSTLWGQGSHWDNATFFDAANNNEVLPAFDFFKKENYE"
BASE COUNT         1070 a          729 c          728 g          995 t
ORIGIN      
        1 acctataccc atactgttgc agagtcctcc ggaactgtag atgttgtgct ggaagcaacg
       61 gacgggactg aaaccgtgag caagggattt acttatgtgc tgagaactcc ttcggaagtc
      121 gcttcaagac ctccgggcac cagaagagga ataaattact ttgccgatga tgaaacaaga
      181 gcaatcctgt gtcttgaggc gcctactaat ttatccgggt atgtaatcgg tgattttact
      241 gactggaaaa tagactccaa ttttaaattg aaacaagacg gggatttttt ttggattgaa
      301 attaccggcc tgactcccgg aacagagtat gcttttcgct acctgattga tgagacaatt
      361 tatatcgcag atccctactc cgataaaatt ctcgatcccg atgatcgatt tattgatgaa
      421 aatatttacc ccggcttaaa aacttttcct gaagcggccc tggtgccgca gtggtacttt
      481 aaccggcttt ccattttgca aacaggacaa gaatattttt tctggcaaaa tgataattac
      541 caaaaaccag aaaaagaaaa gctggtcatc tatgagttat tgattcgtga tttcttcgga
      601 cccggccagg aatcatttca gaatctcatc gatacacttt catacttcaa aagtctgggt
      661 attaacacga ttgaactcat gccggttaca gaattcaatg gaaacgacag ctggggatat
      721 aataatacct tcatgtttgc cccggacaaa gcctatggcc ctaaaaacaa actaaaggaa
      781 ttcattgatg cagctcattc taaaggaata gcggtggttc ttgatgttgt gatgaaccat
      841 catgacatcc cttcacccct gcttttactt gatttcgatt acactacttt caagccggga
      901 tcaaccaatc cctggttcaa tgtggaagcg ccgcattctg taattaaatt tttcttcgat
      961 ataaaacatt caagtgttta tacccagtct tatcttgatt cactgaatca ttattggatc
     1021 aatgagtatc attttgatgg tttccgtttc gatttttcca aagggttcac acaaaaattt
     1081 acttcgagct ttgaatcact ggcacaatat gatccccagc gggttagcat attgaaacga
     1141 atggccaata aaatttggtc ttatagtccg gatacttatg ttatcctgga acactttgca
     1201 gacaataccg aagaaactga tttagccaac tccgggatgt tgctgtgggg taattcacag
     1261 tatgcctaca aacaaaatgc cctgggattt gtagaggatt cggatattga ctggattttc
     1321 caccgcacac ggggctggaa taatgcccac ctggtagggt atgttgagag tcatgatgag
     1381 cagaggttaa tgtttgaaaa tttgaattat ggaaatcaaa gcgggttcta tgatgcgcgg
     1441 aacctgcata ccgctctgaa tagaatgaaa gctattgccg ctactttctt cagcatcccc
     1501 ggcccaaaaa tgatctggca atttcaggaa ctaggctacg atattgatat aaaccaaaat
     1561 ggacgtgttg gcaggaaacc tgttccctgg aatgatgcgg agggtctgga ttataataat
     1621 gaggaggcaa gagttagttt aagaaataat tttgcagaaa tgattaagtt gaagaacgcc
     1681 tattcaatct tttccacggc cgacgtgacc tttctgggtg aaaataccct aatcaggcaa
     1741 atcatattaa aaaacgatcc ttatacgaag accccggcca accacgaaga aatgaatgtt
     1801 gttgttattt ccaatttcga cataataagc aaaaacattt ccgtgaattt cccacatacc
     1861 ggtacatggt ataactattt tgcaaatctg gatgaggttc aagttgaatc tttaccacat
     1921 actgtggaat taaatgccag tgatttcaga atatttaccg attaccgcct cccaaaacca
     1981 gacctgataa catctgttaa tgatagtccg cttacaccag ggattcatat ttatccgaac
     2041 cctgtcaatg agacaatgac aattgatacg ggagaattca tccctttcca gattaggatt
     2101 atttctcttt caggacaaat actattaaat tcaacactgg attcagcgca aaaacattct
     2161 gtagatgttt cattcctgcc caacggaatg catatattag tcatggagtc tgccggagtc
     2221 aattatgtaa caaagattat caagcaataa attactactg tgtatacacc ctccgcaacc
     2281 cgttatgata caatgaaata ccgaaggtgc ggaaaatcgg gaatacaact tcctgtaatt
     2341 tcattgggtc tctggcataa tttcggagat gttgataatc aggagatttg ccggagcatc
     2401 attcaccgtt cttttgattt aggaatcact catttcgatc tcgctaataa ttacggacca
     2461 ccgccaggct ccgcggaaac caatttcggg aatattctga aaaaagattt gtctgcctac
     2521 cgggatgaga tgatcatttc aactaaagcg ggctactaca tgtgggaagg tccttatggt
     2581 gaatggggct caaaaaaata tcttgtgtca agtcttgatc aaagtctgaa acgcctggga
     2641 attgattacg ttgacatttt ctattctcac cgttacgatc cacgcacgcc gctcgaagaa
     2701 accatggaag ccttatattt atctgtcatt caggggaaag cattatatgt cgggatttcc
     2761 aactactcac cggaagaaac taaattggcg tcaggaatct taaaaacgat gggaataaaa
     2821 tacctgattc atcaacccaa atattccatg ttttatcgat gggctgatga tactaaacta
     2881 tttgatgttc tggaaactga gggaattggc gccatagcat tctcgccact tgcgcaggga
     2941 ttacttacca acaaatatat aagcggaatt cctgtggggt caagagcttc caaatctcat
     3001 ggcttcctta aagaaaaaga tatcagcgaa gaaaaaataa ggaaggtcat tgaattaaat
     3061 gtgattgccg gtgagcgcgg gcagtcactg gcccaaatgg cattggcatg gacactcaga
     3121 gattcgcggg ttacttccgc gctaataggc gtaagcagtg taactcaact tgaagataat
     3181 tgcgctacga ttcagaacct ggaattcact gaacaggaac ttcttcggat cgaagaagtt
     3241 ctcaaagatt aattactcgt aattttcctt tttgaagaaa tcaaatgccg gcagaacttc
     3301 gttattgttg gccgcatcaa aaaaagtagc attgtcccag tgagaaccct gtccccataa
     3361 tgtgctgcag gaagttgaaa tccacgccgg ttcccaataa attacacctt gtccaccact
     3421 ttcaagaaca atccttgtta gatcaatgat gtaattccgc tggcctgtgg gagtggcagg
     3481 atattctgca agcagtgcat cagtgcccaa aatattattt gc
//
LOCUS       DASWHX010000252         7045 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_1087971, whole genome shotgun sequence.
ACCESSION   DASWHX010000252 DASWHX010000000
VERSION     DASWHX010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 7045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7045
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1087971"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..551)
                     /locus_tag="VGA21_15265"
     CDS             complement(<1..551)
                     /locus_tag="VGA21_15265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002700937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HEX9651919.1"
                     /translation="MDIEKILKERILVLDGAMGTMIQSYKLTEDDYRGERFKYHPSSL
                     KGNSDLLNLTKPDIIKEIHRKYFEAGADIAESNTFTATSIAQHDYGLEEFAYEINVEG
                     VRLAKEVATEFTKSNPKKPRFVAGSIGPTNKTASLSPDVNDPGYRAINFDQLRAAYKE
                     QARGLMDGGADIFLIETIFDTLNA"
     regulatory      complement(602..702)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(909..1676)
                     /locus_tag="VGA21_15270"
     CDS             complement(909..1676)
                     /locus_tag="VGA21_15270"
                     /inference="COORDINATES: protein motif:HMM:NF024957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HEX9651920.1"
                     /translation="MRIVLILLFVFCSNMVLGQFWIGPKIGIQGYTTTFKFSEARDDY
                     KSYPRMGFTIGGAISANLKPTFSITSEFQYSLKGKHTEIRENEWRNVATFHYLEGPIM
                     LRYIFEEFYYQEVTFRPYIQGGPNFMYWLGGKGNIRNEESSGVFYKISFDEADTVNFS
                     KMSIGGANRFMWGLELGGGVIVNVKQGQIILIDLRFHFGHTFFGDKDGAKYPMLGFSD
                     NLEQHYRLLNLSATYLFEVDLRKMRKGKSTINKRKRR"
     gene            1938..3377
                     /locus_tag="VGA21_15275"
     CDS             1938..3377
                     /locus_tag="VGA21_15275"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HEX9651921.1"
                     /translation="MRKSLIASLALLMSVATFGQEVPTYSHYFINPYLYNPAFAGSEG
                     KGALFLTHRRQWVGIDGAPVTSNLSYHTPFTRGLNFGLNLNWDERSILNSYNGLLTLG
                     YTIKFSRQVFMRFGISGGARWSSIDLDQVDNPSDPALIGILGNSISLDGNAGISLHFG
                     TFSLGASLPHIFSTETISSEPFSTGEFFPTDIILFTASYRQYFAQDQFAFEPRFFYSY
                     SEVGDSQWEALATLDLKNIIYLGGAYKQDYGFTALLGLKIRGSLGIGYSFDFGPAAYS
                     GLKNGTHEFQLSLAFGKGRKRLNQKYSFLDMRRSIEPKPEKPLIVETQKDPVVEEPVV
                     ETPIEEVPVEEEIVEETQPAVEEQPVQEEETEEQIPEVVQPEIVTPEPDGSGDQPVVV
                     RRGGHLLELPEGHYVINGAFTRLENAQNFSDELFQKGYKASFGFVTEKNLYYVFLFKT
                     DNTQSARAERDRLRQNNLFPNAWYLVVEK"
     gene            3414..6614
                     /locus_tag="VGA21_15280"
     CDS             3414..6614
                     /locus_tag="VGA21_15280"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HEX9651922.1"
                     /translation="MIRISQINKLLTLLVFLMISWGVQAQAPRISSIEPEKASAGSLV
                     TITGDGFSTDMTKMIVWFGAARGQIVSSTENIIEVTVPAGATYGSLSVTNTTTNLIGY
                     STRQFLLTYGGKTFDVANLTAPIDFPTSFELFDLCLCDFDGDGKVDVGSASLTTPQPY
                     IFKNTSTGNTPTFSNQRFTVPGVTKAISCIDLNGDGKPEMLLTRGDQISNSFFVLQNT
                     STTGTISFSSNVQSYFSAEERPVNRLAIRDLDGDGLPEVIATNSFNNSIYIFKNESTP
                     TLVKLNSIPQPVTIVGSTDSFDIEAVDLDGDSKPDLVISPFNKENLYIVKNTTTALNN
                     FTFSNATQIIIAGKFREMVMTELNGDTLPDFVIANSSENKIHLGTNTSTSNTFSFSWT
                     EKTSNQPFSLDCGDLNGDGLGDVLASNLAGTDMKILLNNTVLPSTSPTFQEITVSADA
                     VADQSVLFTRQTKIGDISGDGKPDLLAATWDNTFSKYNLTVIRNKNCVEPILNPVGPL
                     AVCSGQTITMSTVSALEATYVWKIDGVDTGAPSQPTYDYVVDGSSPKITVSVVSEAGS
                     CDILSNEVSITFDPGIAPATPTLSDPAAVCVGSDVTLTGPARAGATFFWRGPNGFTSS
                     VQSPVISSATAEAAGTYYLAIQEGNCLSDEASLFVEIVSPPGAAISTAGSSVICQGQS
                     SITLEVTNVSDFSYQWKLDGSPIAGATGVTHDALQQGSYTVALSSNIASCTVETPAFA
                     VNSVPPPVADFNASASGCVGEVIQFENISTVDGAQTATYLWNFGDGSTGSPDSDPTHG
                     FNAAGTYDVTLTVNYGDATCQDIVTKPISIAASPTITISTPSLELCSSDSLVLKASGT
                     YASLIWSTMETADSIQIFTGGEYYATAMNAEGCEAADTVTIVELPSPEVTITADPGTG
                     INLGESVILSATGGLSYSWTPTASLDNPGVATPMATPDSTTIYQVEVTGENGCVTFAQ
                     IEILVGIELNPRKVFSPNGDGIEDDWIIGGIQNMPNCSVVIFDRQGRKVFESQPYNND
                     WKALTPGGGQLPAGPYYFIIKCDNEVAKTGSVLVVR"
     gene            6771..>7045
                     /locus_tag="VGA21_15285"
     CDS             6771..>7045
                     /locus_tag="VGA21_15285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651923.1"
                     /translation="MRVNTRKASCVFVFLIFCAIKVYPQSGLTFIGTGTDCSVLPDPA
                     NNPFTAMLPPVVSQQNTADTPSKVLVVDVDGDGVPESIVAGKFGNNLR"
BASE COUNT         2021 a         1514 c         1464 g         2046 t
ORIGIN      
        1 gcattcaaag tatcaaatat ggtttcaatt aaaaaaatat ccgcgccgcc atccattagt
       61 cccctggctt gctctttgta agccgccctc aactgatcaa agtttattgc cctgtatccg
      121 ggatcattaa catccggaga aagagacgcg gttttgtttg ttggaccgat ggaccccgcg
      181 acaaaccgag gctttttggg attgcttttg gtaaattctg ttgctacctc cttcgccagc
      241 cggactcctt ccacattgat ctcataggca aactcttcca atccgtaatc atgctgagca
      301 atactggttg ccgtgaaagt attgctttcg gcaatatcag ctccagcctc aaaatactta
      361 cgatgaatct ctttgattat gtcaggctta gtaagattaa gtaaatctga atttcctttt
      421 aaggatgatg gatgatattt aaaacgctca ccccgataat catcttcagt gagtttatac
      481 gattgaatca tcgttcccat ggctccatcc aggaccaata ttctctcctt caggattttt
      541 tcaatatcca ttttattacg ttttaactta ctgatttaga tgagtttagg atagatttgc
      601 ctacttatct gtccccattt tactgggtgg gatttggcac ccgccaatca cgggttgcca
      661 agacttcaaa gggcccattc cctcagtctt tctcaataag cggctcaaag ataagtaaaa
      721 tattgggaca atgcagaatg ttggcagctc tctagtagtg tctcagttaa gatcacaatg
      781 aaattaatat ctacgtgtgc cacacttcag tcctttatca ggtgaaattg attgtttttc
      841 cgtgtcgaaa aagtgtggca cacgtataat gacgtataat gattccaaac tgatacacta
      901 cccagattct accttcgttt gcgcttgtta attgtgcttt tgcccttgcg catttttctc
      961 aagtctactt cgaataaata tgtcgctgaa aggtttaata atctgtaatg ctgctccagg
     1021 ttatcactaa atccaagcat tggatattta gcgccatcct tgtcaccgaa gaaggtgtgt
     1081 ccaaaatgaa acctgagatc gattagtatg atttggcctt gcttcacatt cacaattaca
     1141 ccgccgccta actccaatcc ccacatgaat ctgttggccc cgccgatgct cattttgctg
     1201 aagtttacag tatcggcttc atcaaaggaa attttgtaga aaacaccgga actttcttca
     1261 ttccgaatgt tgccttttcc tcccagccaa tacataaaat taggtccccc ctgaatgtac
     1321 ggcctgaatg taacctcttg ataataaaac tcttcaaaaa tatatcttag cattattggc
     1381 ccttccaaat aatgaaaagt ggccacgttt ctccattcgt tttcccggat ttcggtatgc
     1441 tttcctttta gagaatactg gaattcgctc gtaattgaaa aagtcggttt taaattagcg
     1501 gagatggcac ctccaatagt aaaacccatt ctgggatagc ttttatagtc atcccttgct
     1561 tcactgaatt tgaaagttgt ggtataaccc tgtatcccaa tctttggccc aatccaaaat
     1621 tgtccaagaa ccatgtttga acaaaacaca aacagcaaaa tcaggactat tctcatgctt
     1681 caattataag aaagattaaa aactttcttt gaaaacgtta atttaacctt gaaattacat
     1741 aaatttattc caaaatttgg aacaccatta aaaatttctc gaaccagaag acgttgaata
     1801 aagatacaaa ttcgggggta ttaatgtctt tgggttactt tcatggatac aattgagata
     1861 aatataaaag ggggttatct taaaaaaatc taatcaatct taataattgt tctattgatg
     1921 ggtaaacagg tcgcgttatg aggaaaagtt tgattgcctc actggcattg ctgatgtcgg
     1981 ttgcaacttt tggccaggaa gttccaactt atagccatta cttcattaat ccatatctgt
     2041 ataatccggc atttgcagga agcgaaggca aaggagcttt atttctgacg cacaggaggc
     2101 agtgggtagg cattgatggt gctcctgtta caagcaacct atcctatcat actcctttca
     2161 cccgaggtct gaattttggt cttaacttaa attgggacga aagaagtatc cttaattcat
     2221 ataatggcct gttaaccctt ggatatacta taaagtttag ccggcaggtt tttatgcgct
     2281 ttggaatatc gggaggcgcg cgatggagtt ctattgacct ggatcaggtg gataatccat
     2341 cggacccggc attaattgga attttgggta attccatttc actggatgga aatgctggaa
     2401 tatccctgca ctttgggaca ttcagtttag gagcttcatt acctcatata ttttcaacag
     2461 aaaccatttc cagcgagcct ttttccacag gagaattttt tcccactgat ataatcctat
     2521 ttacagccag ttaccggcag tattttgcgc aggatcaatt cgcatttgag cccaggtttt
     2581 tctactctta ctccgaagtt ggcgacagcc aatgggaagc cctggcaact cttgacttga
     2641 aaaatatcat atacctgggt ggcgcataca agcaggacta cggatttacc gccctgcttg
     2701 gactaaaaat acgcggctca ttgggtattg gatatagttt tgattttgga cctgctgcct
     2761 attcaggttt aaaaaacgga actcatgaat tccagttaag cctggctttt ggaaaaggaa
     2821 gaaagagatt aaatcagaag tattcttttt tggatatgcg gcgctcaatt gaaccaaaac
     2881 ctgaaaagcc attaatcgtt gagacgcaga aagacccggt ggttgaggag ccagtggtag
     2941 aaacacccat agaagaggtt ccggttgaag aagaaatcgt tgaagaaacc caacctgccg
     3001 ttgaagagca gcctgttcag gaagaagaaa ctgaagaaca aatcccggag gtagtccaac
     3061 ccgaaatagt aacccccgaa ccggacggaa gtggtgacca acccgtagtg gttcgcagag
     3121 gcggacactt attggaactt cctgaaggtc attatgtaat aaacggagcc ttcacgagac
     3181 ttgaaaatgc ccagaatttc agcgatgaat tatttcaaaa gggatacaag gcctcatttg
     3241 gttttgtaac cgagaaaaat ttatactatg tgttcttatt taagaccgat aacactcaaa
     3301 gtgcaagagc agaaagagac aggttgagac aaaataactt atttccaaat gcatggtatt
     3361 tggttgttga aaagtaggtt actttttgac taattttaat aattaataga ttaatgatca
     3421 ggataagcca aattaacaaa ttgctcactt tgcttgtttt cctcatgatt tcatggggag
     3481 ttcaagctca ggcccccaga atatcctcaa ttgaacctga gaaggcttct gccggctcct
     3541 tggttactat taccggggat ggcttttcca cggatatgac gaaaatgatt gtgtggtttg
     3601 gagccgcacg gggacagatt gtgtcttcaa ctgaaaacat aattgaggtt acagtaccag
     3661 ccggagccac atatggcagt ttaagtgtta caaatactac cactaattta atcggatatt
     3721 ctaccaggca attcctgctc acttacggtg gaaaaacttt tgatgttgcc aaccttacag
     3781 ctccgattga ctttccaact tcctttgaac tttttgattt gtgcctttgc gattttgacg
     3841 gcgatggtaa agtggatgta ggatcggcat cactgacaac accccagccg tatattttca
     3901 aaaatacttc taccggaaat actcctacct tttcgaatca aagatttact gttcccggag
     3961 taacaaaagc gatttcttgt atagatctaa atggagatgg taagcctgaa atgttgctaa
     4021 cacgcggcga tcagatctcc aactcatttt ttgttttgca aaataccagt accaccggaa
     4081 caatttcgtt ttcaagtaat gtgcaatcct atttcagcgc tgaggaaagg cctgttaaca
     4141 ggctcgcaat ccgggatttg gatggggatg gattgccgga ggtgattgcc acaaattcat
     4201 tcaataattc catctatatt tttaagaacg aaagtacacc cacactggtc aaattaaatt
     4261 cgataccaca accggtaaca atagtgggtt caacagatag ttttgatatt gaagccgttg
     4321 accttgatgg agactcgaaa ccagaccttg taatatctcc atttaataaa gagaatttat
     4381 acatcgtaaa aaataccact acggcgctca ataatttcac atttagcaat gccacgcaga
     4441 tcatcattgc cggcaaattc agggaaatgg tgatgacaga gctcaatggg gatacccttc
     4501 ctgattttgt aatcgcaaat agttcggaaa acaaaattca tctgggtacc aatacttcca
     4561 catcaaacac attttcattc agctggacag aaaagacatc caatcaaccc tttagcctgg
     4621 attgtggtga ccttaacggg gatggacttg gcgatgttct tgcctccaat cttgccggca
     4681 ctgacatgaa aatattgctg aataatacag tccttccttc tacatcgcct acctttcagg
     4741 aaattaccgt ttcagcagat gcagttgctg atcaatcagt attatttacc aggcaaacta
     4801 aaattggaga cataagtggc gatggcaagc cggatctact ggctgctaca tgggacaata
     4861 ctttttccaa atacaacctg accgtgatcc gcaataagaa ttgtgttgaa ccaatattaa
     4921 atccagtggg acctttagcc gtctgcagcg gccagaccat aaccatgagt acggtttctg
     4981 ctttagaagc tacttatgtc tggaaaatag atggagttga tacaggcgct ccatcgcagc
     5041 ccacctatga ttacgttgta gatggcagca gtccaaaaat aaccgtgtcc gtagtttccg
     5101 aagcaggaag ttgtgatatt ttatccaatg aggtttctat cactttcgat ccgggtattg
     5161 caccggcaac cccaactctt tctgatccgg ctgctgtttg cgtgggcagc gatgtaacgc
     5221 tcaccggccc cgctcgggcg ggagctacat tcttctggcg tggacccaat ggtttcacct
     5281 cttctgtgca aagccctgtg attagctccg caaccgcgga agctgccgga acatactatc
     5341 tcgctataca ggaaggaaat tgtttaagcg atgaagcatc tttattcgtg gaaatcgttt
     5401 cccctccggg tgcagctatt tccactgcag gatcttcggt tatatgtcag ggacaaagct
     5461 ccattaccct ggaagtaact aacgtttcag atttctccta ccaatggaaa ctggacggaa
     5521 gcccaattgc cggggccact ggtgtaacgc atgatgcttt acagcaggga agttacacgg
     5581 ttgctttgag ctcaaatata gccagttgta cagttgaaac ccccgcgttt gcagtaaaca
     5641 gtgttccgcc acccgttgcc gattttaatg cttccgcgag cggttgtgtt ggagaagtta
     5701 ttcagtttga aaatatttcc acggttgatg gtgcacagac tgctacttat ctttggaatt
     5761 tcggtgatgg aagtacgggt tcaccggatt ccgatccaac tcacggcttt aatgcggcag
     5821 gaacctacga tgtaacatta acggttaatt acggtgacgc gacctgccag gacatcgtaa
     5881 caaaaccaat tagtattgca gcctctccaa ccatcaccat cagtactcct tccctggagt
     5941 tatgttcttc tgactccctt gttctgaaag cctcaggcac ctatgcgtct ctgatctggt
     6001 ctacaatgga aacagctgat tcaatacaga tttttaccgg tggcgagtat tatgcaaccg
     6061 ctatgaatgc ggaaggctgc gaagccgcgg ataccgtaac tatagtagaa ttaccctccc
     6121 ctgaagtaac tataaccgct gaccccggta cggggataaa tttaggagaa tctgttatcc
     6181 tttcagcaac aggaggactc tcctattcct ggacaccaac agcaagtctt gataacccgg
     6241 gtgtggccac accaatggca acaccggatt caactacaat ttaccaggtt gaagtaaccg
     6301 gtgaaaatgg ttgtgtcaca tttgctcaaa ttgaaatatt ggtaggcatt gagttgaacc
     6361 ccagaaaagt attttctcct aatggagacg ggattgaaga tgattggata atcgggggca
     6421 tacaaaatat gccaaactgt tccgttgtca tttttgacag gcaaggccga aaggtttttg
     6481 aatcacagcc ttacaataat gactggaaag ctttaacccc cggtggcgga caattacccg
     6541 ccggtcctta ttattttatc attaaatgtg ataatgaggt ggctaaaaca ggtagtgttt
     6601 tagttgtccg ttaaaggaca ataccttaaa atcttaatga aaaaaaggat tttattccta
     6661 tcttcggaaa tctttcgaat gtaatgtaag cagtagtata gctattgaat tatcggttac
     6721 aaagcaagta acttaaggat ttatagtaat ggaattaatc cccaaccaga atgagagtta
     6781 acacgagaaa ggctagttgt gtatttgtat ttctaatctt ttgtgctata aaggtttatc
     6841 ctcaatccgg attaactttt attggaaccg gcacggattg ttccgtactt ccggatccgg
     6901 ctaacaatcc atttaccgcg atgctgccac cggtagtttc gcaacagaat accgctgata
     6961 ccccatccaa agttttagta gttgatgtag atggagatgg agtgcctgaa tccatagtag
     7021 cggggaagtt tggcaacaac ctgcg
//
LOCUS       DASWHX010000253        46523 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_3299597, whole genome shotgun sequence.
ACCESSION   DASWHX010000253 DASWHX010000000
VERSION     DASWHX010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 46523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46523
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3299597"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(455..688)
                     /locus_tag="VGA21_15290"
     CDS             complement(455..688)
                     /locus_tag="VGA21_15290"
                     /inference="COORDINATES: protein motif:HMM:NF024835.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX9651924.1"
                     /translation="MAKTSKTRNRNNLKAFGDKLQKLRLSKGMSQEDLADESGLAYTT
                     INKLENGHLNTGIATLFDLAKGLKVPPRELFDF"
     gene            784..969
                     /locus_tag="VGA21_15295"
     CDS             784..969
                     /locus_tag="VGA21_15295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651925.1"
                     /translation="MMMELPRDLNLIKLPVNIEIIIYHIREELRTCKAAVSPDGRPDE
                     AWRTSSLASLILCFTCL"
     gene            complement(1509..1883)
                     /locus_tag="VGA21_15300"
     CDS             complement(1509..1883)
                     /locus_tag="VGA21_15300"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEX9651926.1"
                     /translation="MTEKTLQPTDITFQQNISDSIFCYHLRLTDNYNSPDKWTEDAWK
                     TIEQHTNFLENLGKSGILIFAGRTLFLPDDKRLFGIALIKAKNIETAKEILSNDPAVV
                     KGIQQADIFPFSLGIRHFDNIE"
     gene            complement(2142..2543)
                     /locus_tag="VGA21_15305"
     CDS             complement(2142..2543)
                     /locus_tag="VGA21_15305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012926103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX9651927.1"
                     /translation="MRQKIVHISLVVNDYDEAINYYTSVLNFTLIEDTVLTETKRWVL
                     LAPKGSNECCLLLAKAINEEQKSRVGNQTGGRVFLFLQTDNFKRDYENLVDKGVSIVR
                     EPVKETWGTVAVFEDLYGNLWDLIEPPSRKN"
     gene            complement(3004..3831)
                     /gene="tsf"
                     /locus_tag="VGA21_15310"
     CDS             complement(3004..3831)
                     /gene="tsf"
                     /locus_tag="VGA21_15310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010610089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HEX9651928.1"
                     /translation="MITAQDVNKLRQMTGAGMMDCKKALTEANGDFEKAIEILRKKGQ
                     KVSASRADRNTTEGSVFIKTTDDQKEAVLVALGCETDFVAKNEEFQRLGNLILETAFK
                     NRPASLEELLKLPAQNLTVQEKIIELVGKIGEKIEIVDYTTLTGEMVIPYIHAGSKLG
                     VLVALTGTNETDVSQAGKDVAMQIAAMNPVAVNKDDVDQTVIEKEIEIGKEQARQEGK
                     AEEMLEKIALGKLNKFFKENTLLNQTFVKDNNLTVQKYLEGISKNLSVSAFKRVSVS"
     gene            complement(3919..4710)
                     /gene="rpsB"
                     /locus_tag="VGA21_15315"
     CDS             complement(3919..4710)
                     /gene="rpsB"
                     /locus_tag="VGA21_15315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013455482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEX9651929.1"
                     /translation="MARVEYKDLLDAGVHFGHLTRKWDPRMSGFIFMEKNGIHLIDLK
                     KTMECLDSACIAMKGIVRSGRKVLFVATKKQAKSIVGSEAQRLNMPYVTERWLGGMLT
                     NFATIRKSLKKMSSIDKLTKDEAYQNLAKREKLMISREKGKLEKVLGGISDLTRLPAA
                     LFVVDVKREHIAVKEAIKLNIPVIAMVDTNSDPTTIDYPIPANDDAFKSIDLIVKVVC
                     RAVEEGLEERRKDKDDAKLKEQEEVKRKADETGQEEPVKEKEASN"
     gene            complement(4723..5109)
                     /gene="rpsI"
                     /locus_tag="VGA21_15320"
     CDS             complement(4723..5109)
                     /gene="rpsI"
                     /locus_tag="VGA21_15320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010610091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEX9651930.1"
                     /translation="MEQTNKIGRRKTSVARVYLKSGKGIIQINDRKLEDYFPSEILQT
                     IVKQPLEIVNATASYDFNINVEGGGFKGQAEAVRLGIARVLCEENPDNRPPLKKAGFL
                     TRDPRMVERKKYGRRKARKRFQFSKR"
     gene            complement(5113..5562)
                     /gene="rplM"
                     /locus_tag="VGA21_15325"
     CDS             complement(5113..5562)
                     /gene="rplM"
                     /locus_tag="VGA21_15325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002698364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEX9651931.1"
                     /translation="MDSLSYKTKFQNVATVDKKWVLVDADAQVLGRLSSQVAAIIRGK
                     NKPGFTPHVDGGDNVIVINADKVKLTGKKWDQKTYTRYSGYPGGQKSLTAKQVKARSS
                     ARIIEAAVRGMLPKNRLGRAIFNNLHVYEGAEHPHEAQKPKQIKLQS"
     gene            complement(5664..6374)
                     /locus_tag="VGA21_15330"
     CDS             complement(5664..6374)
                     /locus_tag="VGA21_15330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008625596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pseudouridine synthase"
                     /protein_id="HEX9651932.1"
                     /translation="MAQIRFQNLIISEDENHIFLNKPAGISTLEDRFSPINILQMAKA
                     VYPGCQICHRLDKETSGVLLVAKNSDTYKFVSKLFEVRKVKKIYHAVVDGLHDFKNFR
                     NDKSLQQTGRGKSRTSNIGKESTTIFTTLDTYRYHSLVECVPMTGRTHQIRAHLQSMD
                     ASILGDREYGGKDVFLSQLKKRFNLKKFTEELPLIKRMALHAFSIEFKGINGVNHKVT
                     AQYPKDFAVLLKSLAKYR"
     gene            complement(6374..7414)
                     /locus_tag="VGA21_15335"
     CDS             complement(6374..7414)
                     /locus_tag="VGA21_15335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009578818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9651933.1"
                     /translation="MRFNSSSLALIVSILVSALVFFILTSVKTIEKNEISLVILFTFI
                     GGFIIAWVILEFLVFRNINSFVAKLRQERNLNGKSDPGENNNTLAEELNNLLEDKNRT
                     IDELKKLAAFRKEFIADVSHELKTPIFAAQGFVHTLLDGAVKDKSVRNKFLKKAARSL
                     DGLDALVQDLVTLSQMEIGEVRMHREYFDIMQLTQEVIDQFEHKAEKKTLKLLYQGDT
                     KQEILVYADPHRIFQVLSNLISNSINYTDEGGQIYVEIIPNQDHVWIKIKDTGIGIPK
                     EDLARIFQRFYRVDKSRSRKQGGTGLGLAIVKHIIELHKSEVKVESEVGMGSTFSFKL
                     PKEKTPQKKRVN"
     gene            complement(7411..8097)
                     /locus_tag="VGA21_15340"
     CDS             complement(7411..8097)
                     /locus_tag="VGA21_15340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009194854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX9651934.1"
                     /translation="MNTPGKILIIDDENDILELLAYNLEKEGYEVKTAQDGVKGVSVA
                     RKFRPQVILLDIMMPIQDGIETCRQLREIPELSEVYVIFLTARAEEYSEIAAFETGAD
                     DYIVKPIKPRALLSRINAQFRRESKQKVEKNLIQAGNLFIDKNSYVVKIGDKKLVLPK
                     KEFELLYFLAQNPHKVYSRNDLLSNIWGTDVYVLARTVDVHIRKVREKIGEGFIETVK
                     GVGYKFVENQ"
     gene            complement(8134..8955)
                     /locus_tag="VGA21_15345"
     CDS             complement(8134..8955)
                     /locus_tag="VGA21_15345"
                     /inference="COORDINATES: protein motif:HMM:NF022750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HEX9651935.1"
                     /translation="MRFLIFTLALLAFIAWSEKSSDTYRYIPNYVWGPGEKITYRAHY
                     GFITAGTGEMIIHDDIYEFNGRKCYKLEIIGKSGGLFEFVMHIEDYWGTYFDVESIIP
                     HYFYRNLQEGRYRKWETTDFNHETDTATVITFDKVTKKIGTLEKFAVPDNAQDMVGGY
                     YYLRLLDFSKFTKGDTIIVDSFFDDTSYNFKILYLGKEKLNTAIGKINSIILQPIMPK
                     NKLFDGENSITVWISDDINKVPLKARANMFIGAVEIEITGYTAGKRKQRPAKFWE"
     gene            complement(8964..9998)
                     /locus_tag="VGA21_15350"
     CDS             complement(8964..9998)
                     /locus_tag="VGA21_15350"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF021535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEX9651936.1"
                     /translation="MKWIKRSALLILILFLILTVGYFSEIIYGIRQLQGQLKIIKGSI
                     ELDEYLANPSKPDSIKQKLGLVTRVKEFSINILEFNSTDNYTSVYDQKGEDILWVLTA
                     SLPFKLEPKTWKFPFLGEVTYKGHFNLHRAIQEARELEKENWDVNVRTVTAWSTLGWF
                     SDPVLTGMLDRREGELAELILHELTHSTLFVKDSVEFNENLASFIGTKAAKLFLISEY
                     GKDSNQFIEYENGLKDQQKIVNFMLTSASKLDSLYTNFANQTDTAQIKKYKDEMITRI
                     INNLDTVTFENKNEFQDFFRERKPNNAFFMNFVRYNSMEDEFERILASKFDGDLKSFL
                     DFFTTNYPSL"
     gene            complement(9995..11203)
                     /locus_tag="VGA21_15355"
     CDS             complement(9995..11203)
                     /locus_tag="VGA21_15355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651937.1"
                     /translation="MIKFRTLIIIGFFALQCINPSLAQYDNTPFLHHLPQDSSKINSL
                     YLSFETLNFLKNNEYSSYIADGYTLFGSQFLPYFSYFAASKVRVNAGIYLQKDFGNDK
                     FTAIEPYFSIKLSRKNFHLIFGNLEGSLNHKLIEPLYDFERVLNDRQEFGFQAMVDRE
                     KFFGEVWVNWENTIYKGDPVQEEIAGGLSIDKKFNLGKLEISIPLQLFVYHRGGQIDS
                     SPNHLLTRVNATAGVNLNLPLGNFIESINGSAYYINSINGDSKTSNDQRQPPAGPVFP
                     SLPQYPEASKGDTLVYGEGNGFYANAGLNLKHNLNLMASFWIADNFASPKGGQLYPSE
                     TSSYKKAGLIVSRRTLIIARIFHDWYLMDNVIFTTRVEPYWDLNTNAFEFAFGFYLNY
                     RPEFLLWRNK"
     gene            11329..12351
                     /gene="recA"
                     /locus_tag="VGA21_15360"
     CDS             11329..12351
                     /gene="recA"
                     /locus_tag="VGA21_15360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018476909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEX9651938.1"
                     /translation="MSDKAEKLKALQLTIDKLEKAYGKGAVMKLSDEKVLDIPAIPTG
                     SLGLDIALGIGGIPRGRVTEIYGPESSGKTTLTMHCIAEAQKAGGLAAFIDAEHAFDK
                     SYAEKLGIDTENLLISQPDNGEQALEITEHLIRSGAIDIIVIDSVAALVPRGELEGEM
                     GDSKMGLQARLMSQALRKLTGTISKTGCACVFINQLREKIGVMFGNPETTTGGNALKF
                     YSSVRLDIRRIGQIKEASNDVTGNRTRVKVVKNKLAPPFKVVEFDIIYGSGISKAGEI
                     IDLGAELDIIQKSGSWFSYNGNKLGQGRESVKQLIEDNPELMEELESKIKGKIKGEGV
                     NEPVSA"
     gene            complement(12440..13702)
                     /locus_tag="VGA21_15365"
     CDS             complement(12440..13702)
                     /locus_tag="VGA21_15365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020607660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9651939.1"
                     /translation="MKSVVLLKLIVESFRFAWSALMMNLFRTVLSLLGVTVGIFAIIS
                     VFTVVDSLESSIRSSFNFLGDKVIRVEKWPFGLGGEYPWWKYMRRPLATYQDYEFLHT
                     NLSNYSAMTISSARGGQTVKRESNSINAVTILGVSQGYSDVYEVPVEKGRYFTYQELI
                     NGRNVCVVGANLVDALFPNEEPIGKTIKIRGLNHFVIGVLEREGESFLGGLPTNDDNC
                     YIPYADWSKMYYSGSRFGGGATIAIKGRETDIGLVELENEVTGLMRRKRGLKPREEDD
                     FAINRPEALQSFIGSTFDIITVAGWIIGGFSILVGGFGIANIMFVSVKERTHIIGIQK
                     SLGAKNYFILFQFLFEAIFLSVIGGGFGLLLVYFLSFMSLGSLELSLSIRNVAIGLGV
                     SSIIGTVAGIVPAALAARLDPVEAIRMQ"
     gene            13813..14859
                     /gene="queA"
                     /locus_tag="VGA21_15370"
     CDS             13813..14859
                     /gene="queA"
                     /locus_tag="VGA21_15370"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010610099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEX9651940.1"
                     /translation="MKLSEFKFDLPSGLIALYPAENRDESRLMVINRKTKEIEHKTFK
                     DIVSYFDEDDVMVINDTKVFPARLYGNKEKTGAKIEVFLLRELNKSMHLWDVLVDPAR
                     KIRVGNKLYFGEGDLVAEVIDNTTSRGRTIRFLYDGEDEDFYKAIDELGETPLPKYIK
                     RPAEPSDRERYQTIFAKNVGAVAAPNSGLHFTKQIMKRLEIKGVKITPVTLHIGLGTF
                     RPVDVEDLTKHKMDSENFEVKQPTVDLVNTALADKKRIIAVGTTAMRALESSVSASNK
                     LKENGGWTDKFIFPPYEFKICNVLLTNFHMPESTLLMMACAFGGYNLVMDAYKLAIKQ
                     KYKFFAYGDAMLII"
     gene            14867..15544
                     /locus_tag="VGA21_15375"
     CDS             14867..15544
                     /locus_tag="VGA21_15375"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019599837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEX9651941.1"
                     /translation="MAGLKKYVLIVAGGYGSRMGSDVPKQFISIAGKPILIHTFEAFL
                     KYSQDLIFILILPADQIDNWRKICVENSFSTPHIIKEGGATRFQSVKKGLEIIKEDGL
                     VAIHDGVRPCVPQEVISSSFLIAKSKGSAIASVSIRESIRKVVGNNSQVMNRNDFVVI
                     QTPQTFPINLIQKAYEIHEQDFFTDDATVYETAGMPVNLIEGSYSNIKITTKDDLRIA
                     EAFLTHK"
     gene            complement(15596..15817)
                     /locus_tag="VGA21_15380"
     CDS             complement(15596..15817)
                     /locus_tag="VGA21_15380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002996718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2795 domain-containing protein"
                     /protein_id="HEX9651942.1"
                     /translation="MYWTLELASYLEDAPWPATKDELIDYSIRSGAPLEVVENLQELE
                     DDGQPYESIEEIWPDYPTKEDFFFNEDEY"
     gene            16016..17695
                     /gene="ettA"
                     /locus_tag="VGA21_15385"
     CDS             16016..17695
                     /gene="ettA"
                     /locus_tag="VGA21_15385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009186200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HEX9651943.1"
                     /translation="MSEEKIIFSMTGVGKIYPPKKQVLKDIYLSFFYGAKIGVLGLNG
                     SGKSSLLRIIAGVDKDYHGEVVFSPGYTIGMLEQEPKLDPKKNVREVVEEGVSEVVSL
                     LKEFESINEKFADPAVMENPDKMQKLIDKQAEVQEKLDAVDAWELDNRLEKAMDALRT
                     PSGEENVEHLSGGEKRRVALCRLLLQEPDVLLLDEPTNHLDAESVLWLEQHLKQYKGT
                     VIAVTHDRYFLDNAAGWILELDRGEGIPWKGNYTSWLEQKQSRLEKEEKSESKRRRTL
                     ERELEWVRMSPKARQAKGKARLNAYEKLLGEEGKQKEEKLELFIPPGQRLGNKVIEAK
                     KVAKAFDDKLLFDDLNFSLPQGGIVGVIGPNGAGKTTLFKMITGSVKPDSGSFDLGPT
                     VQIGYVDQEHAEIDPNKTVWETISDGNDIIKLGTREMNSRAYVSKFNFTGPDQEKKVG
                     VLSGGERNRVHLALTIKKGANLLLLDEPTNDLDVNTLRALEDALENFAGCAVIISHDR
                     WFLDRICTHILAFEGDSKVRWFEGNFSDYEENKKKRLGDIEPHRIKYRKLK"
     gene            complement(17705..18670)
                     /locus_tag="VGA21_15390"
     CDS             complement(17705..18670)
                     /locus_tag="VGA21_15390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019989904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HEX9651944.1"
                     /translation="MKKGVFFLILFSAAISSFRWGFFAHQRINQLAVFTLPPPLNQFY
                     KAHIKYLSDNAVNPDMRRYAIKEEAPRHYIDLDIYPDSTIEFLQQNWYRVTEKIPADS
                     LNEYGIVPWHIVTMYHRLKDAFLQKDPARILKLSAEIGHYIGDANVPLHTTENYNGQL
                     TNQRGIHGFWESRLPELFFEDYTFFVGKAEYIESPLREAWKAVLSGHQALDSVLRFEK
                     ELSNKFPADKKYTYEERGNSFVRTYSREYATAYHEMLSGMVERRMRASVKMVGDFWYT
                     CWVHAGQPDMSEFIEFDFSEWEKENQRKEREGWLNKIFNAREEGG"
     gene            18783..19037
                     /gene="rpsT"
                     /locus_tag="VGA21_15395"
     CDS             18783..19037
                     /gene="rpsT"
                     /locus_tag="VGA21_15395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015263901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEX9651945.1"
                     /translation="MANHKSALKRIRQTEKKNTQNKYEMKSARTAIKKLRTTTKKDEA
                     EELLKKVTSMLDKLAKKNLIHKNKAGNNKSKLKKYVNSLK"
     gene            19117..19821
                     /gene="radC"
                     /locus_tag="VGA21_15400"
     CDS             19117..19821
                     /gene="radC"
                     /locus_tag="VGA21_15400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009582146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEX9651946.1"
                     /translation="MDKLYEKPYLGIKTWAEEDRPREKLLLKGKTHLSDAELLAILLG
                     SGSRALSAVDLAKLVLNSTDNNLNGLARLSVKDLMKFKGIGEAKAISIVSALELGRRR
                     KEQEPEKLDQISCSSDIYRLMKPVLLDLNHEQFWIILLNRRNAVMKKFQVSSGGVSGT
                     VADPKMIYKTALEYLASSVILVHNHPSGNLKPSQSDIALTQKLKNAGAFLDLPILDHI
                     IFTDNGYFSFADQSML"
     gene            19822..20433
                     /locus_tag="VGA21_15405"
     CDS             19822..20433
                     /locus_tag="VGA21_15405"
                     /inference="COORDINATES: protein motif:HMM:NF019533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HEX9651947.1"
                     /translation="MTRNKIFYFVILIVVLLILFNSFDNGETREEFLDRIATERANKD
                     LLFKSDKESPFKDSLVFTELKYYPPDPDYVIDAKFTKIASPQLITLATSDGATKKYKE
                     YGYAEFELEGQMNKLLLLEMEKPFQNKLFIPFADETSANQTYGAGRYLEADKPNGNIV
                     ALDFNLAYNPYCAYSEEYSCPFPPPENLLSVAIEAGEKKYYEN"
     gene            20512..22926
                     /gene="pheT"
                     /locus_tag="VGA21_15410"
     CDS             20512..22926
                     /gene="pheT"
                     /locus_tag="VGA21_15410"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009582149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEX9651948.1"
                     /translation="MRISLNWLKQYIFLEETPEQLADILTMTGLEVEGIEIFESIKGG
                     LKGLVVGEVLTCVQHPEADRLSLTTVDIGAGKPVQIVCGAPNVAKGQKVVVAPVGTVI
                     YPINAEPFEIKRTKIRGEFSEGMICAEDEIGLGADHQGVIEMKTDLPNGSEVTHYFEI
                     ENDIVFEIGLTPNRGDATSHIGVARDLKAFLRRKLEWPSVDQFKVERNIMNISVEIRD
                     KNACPRYSGISITGINVQTSPRWLQNKLESIGLSPINNIVDITNYVLNETGQPLHAFD
                     ADKIANGRVIVQTLANGTKFTTLDEVERTLSADNLMICDSNGGMCIAGVFGGAGSGIT
                     KETVNIFLESAYFSPDSIRKTSQQHGLKTDAAFRFERGTDPNLTVYALKRATMLIKEI
                     AGGQISSEIVDEYPSQIPDREVEITYKNVDRLIGITIDRENIFSILESLDIKIENKTH
                     DGFTAIVPPYRYDVTREADVIEEILRIYGLNNIKIPPKVSAESLAEFPQKDPINILSR
                     TSKLLAANGFYEILTNSLTKPDYAESVDEIDTSANVAIINKLSEDLGVMRQSLLFSGL
                     EVMAYNINRRQTDLKFFEFGKIYHKKAGGYKEKQCIGLFITGLREKENWIRKDSPVIF
                     HDIIGASQLVVDSLCGEIITQSPSRQSIFSFGCSLKVGDRDIGIAGRINPRILKLFDI
                     SKEIFYSELDWDLLLKISNDNIVYEEVTRFPEVRRDLSLVLDKEVSFEEIRRLAQKQE
                     TFLIKEMNVFDLYEGENIGKNKKALAISFILQDKKKTLTDKVIDKTMKRLMNTFETEL
                     GAIIRK"
     gene            22926..23222
                     /locus_tag="VGA21_15415"
     CDS             22926..23222
                     /locus_tag="VGA21_15415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009582150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651949.1"
                     /translation="MSQERLQNQLFSLERKVRLLVGEYKKLKEEIIQVKGENDTLGRT
                     ILKKEQQIGDFQNKINISKIVDSIAVDDVKAAELKKNIDLYIKEIDKCISHLSR"
     gene            23233..23517
                     /locus_tag="VGA21_15420"
     CDS             23233..23517
                     /locus_tag="VGA21_15420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009582151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEX9651950.1"
                     /translation="MEQLSVKIKIADREYPMKVRPEDEEKVRNAGKVINERLKKYKEQ
                     FGLEDKQDLLAMVAFDSLVDLLNLENNISGADDGVLQKVDEINQLVSQVL"
     gene            23597..25159
                     /gene="rny"
                     /locus_tag="VGA21_15425"
     CDS             23597..25159
                     /gene="rny"
                     /locus_tag="VGA21_15425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009186468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEX9651951.1"
                     /translation="MSEIMIIIASAGALVVGIGLGWLIFSKKFQQEEKKALEKAEIII
                     REAEINAETLKNNRILEAKEKFLKMKEDFEEEARKRKNLILSNESKIKQREASISKQF
                     EDMKRKEGDLDRQKDELNEQLDIVKRRKDELDKFHQQKVSILEKISNLTADQAREQLM
                     ESLVEETKAKASSKIKEIVDEAKLTATKQAKKLVIETIQRTATEHAIENCVSVFNIDS
                     DDIKGKIIGREGRNIRAIEAATGVEIIVDDTPEAIIISGFDPVRREIARLSLHRLVAD
                     GRIHPARIEEIVAKTQKNIEEEIVEIGERTVIDLGIHGLHPELIKMVGRMRFRSSYGQ
                     NLLQHSREVANLCAIMATELGLNPKQAKRAGLLHDIGKVWPEESEKPHAILGMEIAQK
                     YNEHPAICNAIGAHHDEIEMTTLVSPIIQACDAISGSRPGARREVMESYIKRLKDLEA
                     LALSFDGVHKCYAIQAGRELRVLVDAENVNDQKAEMLSFEISQKIENEMQYPGQIKVT
                     VIREKRAVSYAK"
     gene            25485..25703
                     /locus_tag="VGA21_15430"
     CDS             25485..25703
                     /locus_tag="VGA21_15430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651952.1"
                     /translation="MKRVNLLVKTAIVFGLVWLASCKADDILNTCTTCTETTTQVSSE
                     YCGTIVEVQTFEQTLYNTSGQDWTCVRN"
     gene            25857..27767
                     /locus_tag="VGA21_15435"
     CDS             25857..27767
                     /locus_tag="VGA21_15435"
                     /inference="COORDINATES: protein motif:HMM:NF019315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HEX9651953.1"
                     /translation="MLINSIPLRGQAWLDLSHHDFLTNPETKLSGDWEFYWNQLLEPG
                     DFNPSLRPDFIHVPGVWNRQGDYKTLGYGTYRFQIKLPPNQFGLSVYFPIINSSAKIW
                     VNDILVAETGIVNSDKNYYTPQLMGTAVPLPDKAEVVTLVVQVANFTYYTSGIPSPPR
                     VGRSSAIFDRNNRNNGIENFFSGSLIAMCIYQLMLFFLNTHRKPYLFLALICFGVAIR
                     ALIVHGGSFLLPNLYPSVHWEVWKKIEFASVYGMLILFPSYIYYLFPTAASKRIQQTF
                     LVTGIILCIPVIFTPHYVYGMLLEIVHLLLLFAFVYAIYSVIKAWRSGNKDARIICFG
                     IIASFPFILIEIIQNSVFTQYNIEFEFLVEAGVLVFLLFQVYLLGRHYAQAYRRLEVS
                     NRELERMVSNRTIELVTSNSVKDRLLSVMSHDVKSPLNSLQGILNIYNKGALQREEFD
                     NFTKIIEADLHKTSLLVENILFWTKNQIKGVEIKIEKFDLRQLLDEITGLYQTIAGNK
                     GIQLGHNLTNETVINSDRNILYFVIRNLVANAIKFSRENTSVRVEVDYENDTILIRVK
                     DQGVGMNDEILRNLCAPELILSTTGTENEKGTGLGIGLCKEYLEKVNGEIVVDSLEGK
                     GSTFTIIINKKN"
     gene            complement(27764..28177)
                     /locus_tag="VGA21_15440"
     CDS             complement(27764..28177)
                     /locus_tag="VGA21_15440"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9651954.1"
                     /translation="MPNLVFIVEDNPAQQRILREHFEQSLGNYVVKTFDTPQDMMSNI
                     HLKPFAIVLDHFFGEKSQNTGVEYLRELRSIRNKTPVIYYTTLDDDKVRKEVLDLGAE
                     AYIIKNSASLVRLRTTLDLLHEKVSKKGFFKKLFG"
     gene            28294..29310
                     /locus_tag="VGA21_15445"
     CDS             28294..29310
                     /locus_tag="VGA21_15445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020527620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEX9651955.1"
                     /translation="MDSTSIKNIKPLGFPWETQDPFLFCVHHEDFYPEGNEQMGPKAS
                     LAGRNIGQDFTIKDGWRMYHGQNVPGFPAHPHRGFETVTIATKGHIDHSDSLGAAGRF
                     GNGDVQWMTAGRGVQHCEMFPLLREDDKNTMELFQIWLNLPKAKKLTDPYFSMLWNED
                     IPNYNHKDSNGLETRVKVVAGKIAGVQAPPPTPDSWAAALNNEVAIWLIRMNTGAKFI
                     LPPASAAVNRTIYFYSGKTVNIAGQEFDSYNAIELFGDKEVEILNGNVESWFLLLQGK
                     PIGEPVVQYGPFVMNTQAEIQQAFQEYQQTQFGGWPWPGNEHVHAREKGRFAKYPDGK
                     IVER"
     gene            29703..30116
                     /gene="arfB"
                     /locus_tag="VGA21_15450"
     CDS             29703..30116
                     /gene="arfB"
                     /locus_tag="VGA21_15450"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013929748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HEX9651956.1"
                     /translation="MASVSKSKFALLQKELTFTSARSSGPGGQNVNKVNTKVTLRFSI
                     QNSEILSEEERGILLRKLANKITKDGSLILSCQEKRSQVQNKELVIQKFLDLIDKTLK
                     KPKARKSTKPSKTSREKRLQKKKEISEKKKWRGKI"
     gene            30237..31433
                     /locus_tag="VGA21_15455"
     CDS             30237..31433
                     /locus_tag="VGA21_15455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013452634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HEX9651957.1"
                     /translation="MGPGAIVAAAFIGPGTVTTATFAGSSYGYTLLWAIAFSIFATFI
                     LQEMASRLGIVGRIGVGQAIRQKVMQPFIKWGAYFLVIGAIVIGNAAYEAGNITGAAL
                     GFQKYGESISVLGINPLVIIIGILAFSLLFSGKYKVIERSLILMVSIMGIVFLASAVF
                     LQPNLREVLKGLFTPLLPEGSLMMVIGLIGTTVVPYNLFLHASTVKEKWKSASDLKTS
                     YWDTLISVTAGGIITMAILITSAIAFEGEAKATSIADLSYQLTPLLGNWSEYFIAVGF
                     LAAGLSSSITAPLAAAFATSEILGWNSTMKGKNFRSIWIFVLVTGIVFSSLGFNPTSV
                     ILFAQVANGMLLPIIAIFLVWVMNDKRIIGEYVNSKVINFLGILVIIVTLILGLKGIL
                     SATNFL"
     gene            31445..32215
                     /locus_tag="VGA21_15460"
     CDS             31445..32215
                     /locus_tag="VGA21_15460"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF003814.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HEX9651958.1"
                     /translation="MKNLEIDINCDMGESFGQFKIGNDEGIFPHITSCSIACGFHGGD
                     PLTIENTIIGALKNGVNVGAHPSYPDVPGFGRRKMDLQKAELKALIKYQIIAVKGLAE
                     CLGAAITYVKPHGALYNSMVINNEETKWVIEGIKEIDNQLMLMGLAGSQVESISKEMN
                     IEFIAEAFADRRYEPDGTLMSRRKKNSIISDPNQAADQVISIIKNNSVESLDGTLVPI
                     KARSICIHGDNPKAVEILTAITIKLAENNIIKKSFASR"
     gene            32212..32925
                     /gene="pxpB"
                     /locus_tag="VGA21_15465"
     CDS             32212..32925
                     /gene="pxpB"
                     /locus_tag="VGA21_15465"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HEX9651959.1"
                     /translation="MTIIPYGDSALLINFEQVIDPQVNENVIELAKVIEHSNIAGVTF
                     LVPAYCSLAIGFQPERITFQQLKKLTENLNSNISKHQHPESKKISIPICYESEFALDK
                     QEVMSYTNLSWDEIKQLHLSISYRVYMLGFLPGFVYMGSLPEKLESPRKKIPRLKVPA
                     LSVGLAGFQTGIYPLDAPGGWQIIGKTPIRIFDNRNRNPFLFEPGMLVTFKEITEDEF
                     YYMATEVDNRNFDLSSLYG"
     gene            32918..33847
                     /locus_tag="VGA21_15470"
     CDS             32918..33847
                     /locus_tag="VGA21_15470"
                     /inference="COORDINATES: protein motif:HMM:TIGR00724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HEX9651960.1"
                     /translation="MDSIITLSIIRPGLNSMVQDAGRSGYQKFGVPVNGFMDRSSARI
                     ANWLVGNPLNDPLLEITMLGPQIEIDNSCSIAVTGADISPMLNGELIPMYETHRVASN
                     STLSFGKLNSGCRTYISFSGKLDVKQWLGSASASAHETQNLTPQSLLTKNMNIRLISS
                     GSLIERRFPWKSKFESKIVARILPGPEYNYFSGETLSHFLNHEFVVGINSNRMGYRLE
                     GTVRGFNPDKEVISSAVIPGTIQITNSGSPIILMADAQTSGGYPRLANIISADLDKVA
                     QLKPGDTIAFQLTTLEKALEAKKVYEHYLNQLK"
     gene            33968..36367
                     /locus_tag="VGA21_15475"
     CDS             33968..36367
                     /locus_tag="VGA21_15475"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9651961.1"
                     /translation="MIQNYFKIAWRQIIRHKGFSIINIIGLSISLAVGLVIIMMVTDQ
                     LLYDTYNRNKERVYRINTEVLRKNEVSSLNKYATTSLPVGHQLAENYTGIVKSARLLR
                     GFGNGWVEFDPDNDLNIPLGGFFADPEIIEILDLEFEFGNPVTALVNPYSVVITRQAA
                     NKLFKEKNPVGELIKVGELGEYKVTGVLKNTQSKSHVIFEALASISTTPTLIAEEKLR
                     PDRDSWKNCTAGWVYVLLTENKNPQEVDVYLREISQKFGSENDDRNFRYYLQSITEIN
                     PGPFLANQIGPGMPMIMVYFLGGLALIVILTACFNYTNLSIARALNRAKEVGIRKVSG
                     AKRRQIIWQFLGESILISLFALSLAFILLIFIQPAFRNLSFSRILQWDLTINLPAIFA
                     SVIVAITVGILAGILPAMLLSSFQPIKALKNLSGMKLLSRVGLRKGLLAVQFGLSIIF
                     IISVHLIYNQMNYMMNADYGFNYENNVRLALGESSWRNMKSELLSYSDIENVSVVSHI
                     PAAGTTYGDTFFKELNDPRSMDYFSVDQDFIENLELQLLAGRNFPEQLSEGETMIIIN
                     EKAVDEFGYSSPLDAIGQTLYNADTVAVQIIGVVKNFHHALMMQQIEPMALRYRPQQF
                     KIIQVKMANPEAIASVVAAWEKVNPGKLINYKLMGDEIKELYQLVFGGLAKIISFISF
                     LAIVISCLGLLGMATYTIETRLKEISIRKVLGSSDWASIYLLSKGFIALLGITIVFAV
                     PLAILINNLWLQLIAFRVGIGASVVVTSILIIMILGLFTIGSQTLRALKVNPAEILRN
                     E"
     gene            36617..38968
                     /locus_tag="VGA21_15480"
     CDS             36617..38968
                     /locus_tag="VGA21_15480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020530825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9651962.1"
                     /translation="MLKHHLLLAFRNFLRYKSSFLINLVGLSTGLACVLLIYLWVDDE
                     LHMDKFHEKDASLYQAMEHQKYAEEIMTTTSTPGILAEALAEEIPEIEYAATATWVSD
                     FTLSIGEKNVGAKGYYVGKDYFNIFSLGLIHGDANHVLLDKNSMVISSELSAKLFETT
                     EGVIGKEVQLQHDKTFLVSGVFEGTPRQSSIQFDFVLSIELFKDENQWANSWGNNGPS
                     TFAVLIDGADVDEVNYKIEDFIQKKNEQSNVRLFLMPYSERYLYGRFRDGKPAGGRIE
                     YVRLFSIIAIFILVIACINFMNLSTARATRRAKEVGIKKAVGAYKGTLVSQFLTESIL
                     ITFISLLTAIVIVKLILPQFNIITEKQIELIFTPTLILWALGITIFTGLVSGSYPAFY
                     LSAFKPVRVLKGDVKGTIGELWARRGLVIFQFAMSTILIFCVLVVYNQIQYVQNKNLG
                     YNKDNIIFFQMEGRVESNRETFLSEVRKLPNIVSAATIGHNLLGRQNNTSGLEWDGYN
                     PEEKILFENMRVGYDLIETLGIEMKEGRSFSRDFATDPIKIILNEAAIRTMNYENPVG
                     RTMKLWGKEREIIGVVKDFHYQSLHSEVNPLFFILAPEVTWYIMVRIEAGTEKTTLNQ
                     LAGFYNEFNPGFTFDYEFLDQSYAGMYIAEQRVASLSQYFAGFAILISCLGLFGLAAF
                     TAQRRLKEIGIRKAMGSSVSSIVLLLSKDFTQLVGISIVVALPVSYFLMSMWLSRFAF
                     KIELSWIYFVGSGLIAILISWLTISIQSFKAARVSPTQCLRNE"
     gene            complement(39022..39783)
                     /locus_tag="VGA21_15485"
     CDS             complement(39022..39783)
                     /locus_tag="VGA21_15485"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX9651963.1"
                     /translation="MEGISAFEITIYVLAGIITAIVNTLAGSGTIFSFGALLYLGVPI
                     DLANTTNRIGVFFQNVTAIITFRSYGKYSLRNLKLQYVIPTLAGAMIGAFAAINVSER
                     FLDIVAGIAMVILLFDAIFDFKKYIKNLHWFNTHTAWLSVLLFFIIGLYGGFIQIGIG
                     IMVVFAIHLVSDSDLVESNFLKLIIILVYTVPTTIYFIYQGKIIWIPALALTVGQIIG
                     AIAAGIFSNVSTNAGFWIRRLLVLMILATLIKVLL"
     gene            complement(39833..41494)
                     /locus_tag="VGA21_15490"
     CDS             complement(39833..41494)
                     /locus_tag="VGA21_15490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008200634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX9651964.1"
                     /translation="MKQILIFSPIILMLSCAQEKEPFTREDVQSAQKIIGLQFEGWEI
                     DTMYSYLLRNRRGYDTMRTYTINHEIAPPLLFDPRPDSFVMPESGPDSLHLEMEEVEL
                     PGSDAGIAFLPVYKLAALIQSGKMTSSELTRIYLERLKTYGDTLEAVITLTEKTALEQ
                     AARADEEIANGKYRGYLHGIPYGIKDLFSYPGYPTTWGSFPYKEQQIEITAAVIERLE
                     HAGAVLIAKLTSGALARGDVWFGGKTRNPWDLNQGSSGSSAGAGAAVAAGLVGFAIGT
                     ETLGSIISPSSRCGATGLRPTFGAVSRYGCMALSWSMDKAGPICRHALDCAIVLKAIK
                     GEDIRDRTTRQFTLIFNPATDLGGYKIGYLKEDFERDSSDVSSNKDRTLETLKLLGAN
                     LEEVKLPDGQPYQVFDIILRAEAGAFFDELVLSHQDRTMVEQDRGSRANSLRQSRFIP
                     AVEYLQANRHRKKLMEAFHEVIKEYDFIIAPTFRSRQSIITNLTGHPAISVPDGFDEK
                     GNPTSVTLIGNLFDDGKILEAAYIFQKATDFEESHPELFMNNQSN"
     gene            41616..42752
                     /locus_tag="VGA21_15495"
     CDS             41616..42752
                     /locus_tag="VGA21_15495"
                     /inference="COORDINATES: protein motif:HMM:NF020965.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HEX9651965.1"
                     /translation="MVQRHLGISVCLILLVYAFRSNGQVEQPHNALAASYQAGYVFGT
                     NGFLRGENRKKEDINFYQNASFKFGRQTDGSELWHRRYNFPFYGAGFHVADYNSPDEI
                     GTPFTVFGFCSFPLHRWQNLSLIYDLEVGVSFNWEPYSIEHNPYNVVMGSKGQGYVGM
                     GFFADFKLSKRWIMNAGVHFAHYSNGAVKRPNLGINSISPKVILRYNLYEDVINYDHR
                     DVPEFDSNNEWTVSLYAGEKQIITETTLDSILRFKREKHQVYGLTTSYHRKISYKSKF
                     GIGTSFSYDGSNNSPDSAWTDNLQWSIFPSYELDVGKFTIIIQPGFYLVRTQTLFNSE
                     RWYQRVGFKYHILENLFVGVNVRAFYFSAADYLEWNLGYRLNWH"
     gene            complement(42777..43934)
                     /gene="galK"
                     /locus_tag="VGA21_15500"
     CDS             complement(42777..43934)
                     /gene="galK"
                     /locus_tag="VGA21_15500"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014019989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HEX9651966.1"
                     /translation="MNHKLISNKFREIYDQEPIIIRSPGRINLLGEHVDYNEGIVLPA
                     AIDKAIWIGVAVSDNAESAIYAIDLEETFSFSLDKIERSAKDWPNYVLGVIDQLIKNK
                     YEFTNFNAVFGGDIPLGAGLSSSAAMECGFLIALKELFNLDLDLLSITKMGQLAEHTY
                     VGTKCGIMDQFANVFGKEDHIIRLDCRSLDYSYHKLALGSYSLVLLDTGVKHSLAESA
                     YNKRREQCDEGVKILRNKFGIMESLRDCDMDQLKKVKHEMAEEVYKRCYYVVTEMQRV
                     ELAIRTLESDNLNELGGLMYETHRGLSNYYDVSCEELDFLVDEARSFGALGARMIGGG
                     FGGCTLNLVKISETENFVGQLTKRYKVQFNLKLQAVPVTIKDGTSVFSTAQ"
     gene            complement(44154..44963)
                     /locus_tag="VGA21_15505"
     CDS             complement(44154..44963)
                     /locus_tag="VGA21_15505"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HEX9651967.1"
                     /translation="MALKDFPLNSPYPVTEEQKKFYNDCGYILVNRLANSAEIYYYRN
                     AIREAVERNKEEKRPLSERDTYGKAFLQIKNLWLENETVKKFVLAKRFGSVAAKLMGV
                     ERVCLYHDQALFKEAGGGHTPWHQDQFYWPLDTDKTITMWMPLVDLTLEMGIMQFVTG
                     SHKQGYLIAQEISDDSDDFFVNYIRENNLEVSRYQELKAGDATFHSGWTLHNAPPNKT
                     DSTREVMTIIYYAEGTRILKPDHDWRQKDLDAWLGGRKPGEVAASSMNPVI"
     gene            complement(45028..>46523)
                     /locus_tag="VGA21_15510"
     CDS             complement(45028..>46523)
                     /locus_tag="VGA21_15510"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M20 family peptidase"
                     /protein_id="HEX9651968.1"
                     /translation="NFIKSEYWNEDFFYFSKKYCVMKKLGYGFLLLSLAVLIILMLNT
                     STFESKQITVDQVTIEIPESPENNLAEAIKFKTVSYEDTSKLDTAEFHSFHKFLKSAY
                     PLIDSLIPMEKVNESLLYKWDGTNKALNPIILMSHQDVVPVDENTLDQWEVDPFKGEI
                     KDGMVWGRGAMDDKVSLIAIMEAVEFLISKGFEPARTIYMAFGHDEEVGGPKGAGEIA
                     KLLQSRGVKAELVIDEGGYVVDKLMSGIDKSLALINVAEKGFVSFKITVNTSGGHSSS
                     PPVDNTIGLLTRAINRLEENQFEYKLVEPINSQMKYMGPELPFLQKVVFANPWLFGGT
                     VAKAMNAHTTTAPTIIQGGMKANVIPTTAWAIMNFRILPGENIELIHEHVVNIINDDR
                     FTIEIDGSAKNPSPVSTIESPAFELIAKTIKQSLGETIVAPGLLGGGTDATHFYSITN
                     NVYRFLPMRINPENLTGFHGINESITIDNYHEIVRFFVQLIRNCNEL"
BASE COUNT        13937 a         9269 c         9241 g        14076 t
ORIGIN      
        1 gtatgggaca actgattaag gaatattatt tcaatgaggc gactgaagtt gaacttgagc
       61 ttccccaatt gcaaggtgga atttactacc ttgaattcct gagtgtgaat cagcgagtgt
      121 tggtgcgaaa gattattaaa gaataagtcc ggataatcat tgtagcgcta tgtccttttt
      181 ggtgtttcca cttaccctca gatactccga agggacactg aagcattaaa aagtggaagg
      241 gtggcacacc ttgatgcacg tactcaagcg catgtaacag tgggcctttt aggtgtgcca
      301 cactttgctg acattaaaat caatcaattt tcacctaaaa taaaaagtgg aagtgtggca
      361 caccttgatg tttacaaaaa ttcaactggc agtttcgatg cggtggctca gcagaccctg
      421 cgaagacaat aaatagggtt agcagtgatc atgctcaaaa atcaaataat tctcttggag
      481 gtacctttaa gcctttggct aaatcaaaga gcgtggcgat gccggtgtta agatgcccgt
      541 tctccagctt gtttatagtt gtatacgcca gacccgactc atccgccaaa tcttcctggg
      601 acatcccttt tgaaaggcga agcttttgaa gcttatctcc aaatgccttc agattgtttc
      661 tgttgcgggt tttagaggtt tttgccacat agcaaatctg ctataaaata aaatccggcg
      721 gtatagccaa cctgctatgg atttgatata ttgaagccat taaaccaact gctggaatga
      781 gcaatgatga tggaactgcc aagagacctg aacctgatta agctgcccgt aaatattgaa
      841 ataattattt atcacatccg cgaagagtta cgcacttgca aggctgctgt ctctccggat
      901 ggcaggcccg atgaggcctg gcgtacctcc agcctggcat cccttatttt atgctttacc
      961 tgcctttgac tgcaaagaca atctgaatat aaatttgcgt gtaggtgttg ggcatatttc
     1021 aggtcgctcc tacggagctt acttattgca ttgttttatt ttctacaaac tggagggtcc
     1081 ctacgggact taacgggttt aactattttt catttcttcg acagaataaa tctacccatt
     1141 gcacaacggt tagcgtaaga aagtacagac tgtgttggcc ggtaataaaa tatttatctc
     1201 ctgacatatt tatttttacc gccaagttaa agctaatgtg cgttacagac gcacattccc
     1261 ctggcacctc gtggaacacg agggccatgt tcggggattt taaattttct attgtttaat
     1321 aatattgagc cattcaaacc aagcacgtaa ctccgcagag tagctaccaa tggtataggg
     1381 cgtttttttc actttcacta tttcctctct ttactatacg ttcagctggt aatttaagta
     1441 accgaagctt ttgagatagg taaaattttc gggccacatc ttgcagtttg cagtcgtctc
     1501 tgtccttatt attctatgtt gtcaaagtgg cggataccta atgaaaacgg aaaaatgtct
     1561 gcttgttgaa tacctttcac tacagcgggg tcattggata aaatttcttt agcagtttct
     1621 atgttttttg ccttaatcag tgcaatccca aaaagtcgtt tgtcatctgg tagaaatagt
     1681 gtccggcctg caaaaatcaa aatgcccgat ttgcccaaat tctccaaaaa gtttgtgtgt
     1741 tgctcaatgg ttttccaagc atcctctgtc catttgtcgg gagagttgta gttgtctgtc
     1801 aaacgcaaat gatagcagaa tatgctgtca gagatgttct gttggaaagt gatgtctgtc
     1861 ggttgtaaag ttttttcggt catatgtttt tctttggagt tatcacattg cacgcaggac
     1921 attgtcagca aaagtccgaa taaatgaatt gtcaagtgtc gcatgcttgt cttcatttaa
     1981 aatacctata acgttcccgg cgctcaaaga aaggcgggca gtgagcgttt cgctttccct
     2041 gccgatgagc tgcaccacgg gggatactct ttaaatatag aaattttcac accactttta
     2101 ctcgcacgaa tattagcggc tggcattttg tcacaaatag tttaattctt tcttgaagga
     2161 ggttcaatta agtcccaaag attgccatat aagtcttcaa aaacagcaac agttccccag
     2221 gtttctttta caggttctct tacaattgaa actcctttgt ccactaagtt ttcataatct
     2281 cttttaaaat tgtcagtttg aagaaataaa aaaactcggc ctccggtctg attacctacc
     2341 ctgctttttt gttcttcatt aatagctttt gctaataaca ggcaacattc attcgaacct
     2401 tttggagcaa gtaacaccca tcttttagtt tctgttaaga cagtatcttc gattagagtg
     2461 aaattaagta ctgaagtgta ataattaatt gcctcatcat aatcgttaac aaccaaagaa
     2521 atatgtacaa ttttttgtct cattacttgt caagttcgcc taaaaaaaac ctgtcattgt
     2581 tcatcaatta accctggctg tcgattgaat gcttctgact aacagccctg gctaagaaac
     2641 gtggagcttt tcggagtgat ttcctgtccg ccccatgttt tcttaggtgc cgttataggt
     2701 cgttttttct tattttcttg tccattatat aaataatata acactataag aaatcaaatt
     2761 tgctgttccg tgagcaatcc atccaggaat aatactacca aacgctaatt tttcgttaat
     2821 atatgttttt agataaccag gcacggatat gaatatccgc gctcactgcg ggtttcaaat
     2881 ccatcgagat tttttgaatt gactgggttt agctaaattt tcgtgtaaga ttttattgag
     2941 tatctgccaa aaataaaaaa gccatcttac cgatggcttt ttatgtcatt tcaagttggt
     3001 tcttcaactt acacttactc ttttaaatgc gcttactgaa agattttttg aaattccttc
     3061 caggtatttt tgcactgtta agttattatc ctttacaaat gtctggttaa gcagtgtatt
     3121 ctctttaaag aatttgttga gttttcctag tgcaattttt tcaagcattt cttccgcttt
     3181 cccttcttgt cttgcctgtt ctttgcctat ttcaatttcc ttctcgatca cagtttgatc
     3241 cacatcatcc ttattcacgg caaccggatt catggcagca atttgcatgg caacatcttt
     3301 ccccgcctgt gacacatcag tttcattggt tcctgtaaga gctaccagca ctcccagttt
     3361 actgcctgca tgaatgtaag gtattaccat ttcacctgtc agggtagtat agtccacaat
     3421 ttcgattttc tctcctatct tacctaccaa ttcaattatt ttttcctgaa ccgttaggtt
     3481 ttgagccgga agcttaagta attcctccag tgatgcaggt ctatttttaa aggcagtctc
     3541 gaggatgagg ttacccaacc tctggaattc ttcattttta gctacaaaat ccgtctcaca
     3601 tcctaaagcc actaaaactg cttctttctg gtcatcggta gttttaataa agactgaacc
     3661 ttcggtagta tttctatccg ctcttgaagc agacactttt tgacctttct ttctcaatat
     3721 ttcaatagcc ttttcgaaat caccattggc ttctgttaat gcttttttgc agtccatcat
     3781 gcccgcaccg gtcatttgtc tcagtttatt tacatcttgt gctgttatca tgacaatttt
     3841 ttttgaattt taaataataa aaaattgaac accggaagct ccaatgttca attcataaaa
     3901 ataatttatc agaatttctt aattagaggc ttctttttct ttcactggtt cttcctggcc
     3961 ggtttcgtca gcttttcttt tcacttcctc ctgctcttta agctttgcgt catctttatc
     4021 tttcctacgc tcttcgagcc cctcttccac cgctcggcaa acaactttca ctattaaatc
     4081 gattgactta aaagcatcat catttgccgg tataggataa tcaatggttg ttggatcaga
     4141 gtttgtatcc accattgcaa taaccgggat attcaacttg atggcttctt taacggcgat
     4201 atgctcacgc ttaacatcca caacaaataa ggctgccgga agtctggtta ggtcagaaat
     4261 tcctcccagc actttttcaa gtttgccttt ttcccgggaa atcatcaatt tttctctctt
     4321 agccaggttt tgataggctt catcctttgt caacttatca atagatgaca ttttcttcaa
     4381 tgacttcctg attgtagcaa agttagttag cattccaccg agccatcttt cggttacata
     4441 gggcatgttt aaccgttgcg cttcagatcc cactattgac ttagcttgct tcttggttgc
     4501 aacaaacaga acttttctac cagatcgcac aattcctttc attgcaatgc aagcgctatc
     4561 caggcattcc atggttttct tcagatctat caggtgaatg ccgttctttt ccatgaaaat
     4621 aaacccggac atcctggggt cccactttct tgtgaggtga ccaaaatgaa ctcctgcatc
     4681 caataaatct ttatactcaa ctcttgccat ttaatgcaaa ttttatcttt tactaaattg
     4741 aaatcttttt cttgcttttc ttctgccgta tttcttacgc tctaccattc ttggatcccg
     4801 ggtaaggaat ccagcttttt tcagcggggg gcggttatcc ggattttctt cacataaaac
     4861 tctggctatt cctaaccgga ctgcttcagc ctgacctttg aacccaccgc cttcaacatt
     4921 aatattaaaa tcataactgg ccgtcgcgtt tactatctca aggggttgct taacgatagt
     4981 ctggaggatt tcggatggaa aataatcttc caacttccga tcattaattt gaatgatacc
     5041 cttgccagat ttcaggtaca ctctggcaac tgaggtcttc cgtcttccaa tcttattagt
     5101 ttgttccatc gattacgatt gcagtttgat ttgtttaggt ttttgtgctt catgaggatg
     5161 ttctgcgcct tcataaacat gaagattatt aaatattgcc ctccccaatc tgttttttgg
     5221 aagcatacct ctcaccgccg cttcaattat cctggccgag gatcttgctt ttacctgttt
     5281 agcagttaaa ctcttttggc ctcctggata tccactgtat cgtgtatagg tcttctgatc
     5341 ccacttcttt cctgttagct ttaccttatc cgcgttaata acaataacat tatcgccgcc
     5401 atcaacgtgg ggagtaaacc cgggcttatt ctttccccga attattgcag ccacctggct
     5461 cgataatcta cccaacacct gagcatctgc atctaccagt acccatttct tatctacggt
     5521 tgcaacgttt tgaaatttcg tcttgtaact taaactatcc acttttcctt attgttaatt
     5581 ttttcacagg cctttaaaag gaggcacaaa gatagagtct tctcccctga aatacaatat
     5641 gcatttatgt ctttttggca attttagcgg tacttggcta aggattttaa caagacggca
     5701 aaatccttgg gatattgagc agtgacttta tggttgactc cattaatgcc tttgaattct
     5761 atggaaaatg catgcaaggc cattcgtttg atcagcggga gttcctctgt aaattttttc
     5821 aggttgaatc ttttcttcaa ttggctcagg aatacatctt tcccgccata ttcgcgatca
     5881 ccgaggatag aagcatccat ggattgcaga tgcgccctga tttgatgggt tcttcctgtc
     5941 attgggacac actcaaccag ggagtgatac cggtaggtat ccagcgtggt gaagatggtt
     6001 gtagactctt ttcctatatt ggatgttctg ctcttgcccc ttccggtttg ctgaagagat
     6061 ttatcatttc tgaagttctt gaaatcgtgc agtccatcaa ccaccgcatg gtagattttc
     6121 tttaccttcc ttacttcaaa taatttagag acaaatttat atgtatcgga attcttggcc
     6181 accaaaagaa ctcccgaggt ttctttatcc agccggtgac atatctggca tcccggatat
     6241 acagcttttg ccatttgtaa aatgttaatt ggcgaaaacc tgtcttccaa agttgatatc
     6301 ccggccggct tatttaaaaa gatatgattt tcatcttcac tgataataag gttttgaaat
     6361 cgaatttggg ccatcagtta acccgtttct tttgaggggt cttctctttg ggaagcttaa
     6421 aagaaaaagt cgagcccata cccacttcgc tttctacttt tacttccgac ttatgcagtt
     6481 caatgatatg ttttacgatc gccaagccaa gccctgtccc tccttgcttt ctggatcgac
     6541 ttttatccac tctgtaaaat cgctggaaaa tgcgcgccag atcttctttt ggaataccaa
     6601 tgcctgtatc ctttattttt atccagacgt gatcctggtt cggaattatt tcgacataaa
     6661 tttgtccgcc ctcatctgta taatttatac tattgcttat caggttagat aacacctgaa
     6721 atatccggtg gggatccgca taaaccaaaa tttcctgctt tgtatctccc tggtacagga
     6781 gtttaagagt tttcttttcc gctttatgtt caaattgatc tattacttcc tgggttagct
     6841 gcatgatatc aaaatattct ctgtgcatcc tcacctcacc aatttccatt tgagaaagag
     6901 ttacaaggtc ttgtaccagg gcatcaagtc catcaaggct acgggctgct tttttcaaaa
     6961 atttattacg aacggattta tctttgactg ccccgtcaag aagggtatga acaaatccct
     7021 gggctgcaaa aattggtgtc ttaagttcgt gggaaacatc tgcaatgaac tcctttctga
     7081 aggctgccag ttttttaagt tcatcgattg ttctattctt atcttccaat agattattca
     7141 actcttctgc taaagtattg ttgttttccc cgggatctga tttaccattt aagttccttt
     7201 cctgtcgaag ctttgcaaca aatgaattaa tatttctaaa gacaagaaac tccaggatca
     7261 cccaggcaat aatgaagccg ccaataaatg tgaataaaat aactaaagag atttcattct
     7321 tctcaatagt ttttacagaa gttaaaataa aaaaaaccag ggcgctaacc agaattgaaa
     7381 caatcagcgc cagggatgag ctgttaaacc tcattgattt tcaacaaatt tatacccaac
     7441 acctttaact gtttcaatga atccttcacc gattttttcc ctcacttttc ttatatgaac
     7501 atcgactgtt ctggctaaaa cataaacatc agtgccccaa atattagaaa gcaaatcatt
     7561 acgtgaataa accttatgtg gattttgtgc aagaaaatat aagagttcaa actccttctt
     7621 gggcagtact aatttcttat ctccgatctt caccacatag ctatttttgt cgatgaataa
     7681 gttaccggcc tggatcagat tcttttcaac tttctgcttc gactcacgtc tgaactgagc
     7741 attgatccgg ctaagcaagg ccctgggttt tatgggtttt acaatataat cgtcggcacc
     7801 agtttcaaaa gcagcgattt cggaatattc ttcggccctg gccgttagaa atattacata
     7861 gacctctgat agttcaggta tttcccgcaa ttgacggcag gtttcaatgc catcctgaat
     7921 aggcatcatg atatccagta aaatcacctg gggccggaat tttcttgcta ccgaaactcc
     7981 tttaacccca tcctgagccg ttttaacttc gtatccctcc ttttcaaggt tgtaggccag
     8041 tagctctagg atatcatttt catcatcaat gattaaaatt tttcctggag tgttcactaa
     8101 attaaatttg atcaaaatta ggcattattg ggctcattcc cagaacttag caggtcgctg
     8161 tttccgtttg cccgctgtat atccggttat ctcaatttcc accgcaccaa taaacatatt
     8221 cgccctggct tttagcggca ctttgttaat gtcatctgaa atccacaccg tgatagagtt
     8281 ttctccatca aacaatttat tttttggcat gatgggttgc aatattatgg agttaatctt
     8341 tcctattgcc gtatttaatt tttctttgcc caggtaaagg attttgaaat tgtaggatgt
     8401 gtcgtcaaaa aaactatcca caataattgt gtctccttta gtgaattttg aaaaatcaag
     8461 aagtcttagg tagtaataac ctcccaccat atcctgtgca ttatcgggaa cggcaaactt
     8521 ttccaaagta ccgattttct tggtaacttt atcgaaagta attaccgtcg ctgtatcggt
     8581 ttcatggtta aaatcagtgg tttcccattt tctgtatctt ccttcctgaa gatttcgata
     8641 gaaatagtgt ggtattattg attcaacatc aaaataggtt ccccagtaat cttcgatatg
     8701 cattacaaac tcaaataaac ctccggactt tccgattatc tcaagtttat agcattttct
     8761 cccattaaat tcatagatat catcatgaat gatcatttca cctgttcccg cggtgatgaa
     8821 accataatgg gctctatagg taattttctc cccgggaccc caaacgtaat tgggtatata
     8881 ccggtatgtg tctgaagact tctcagacca ggctatgaat gccagtaaag caagagtaaa
     8941 tatgaggaag cgcactatca aatttacaaa gaggggtaat tagttgtaaa gaaatcaaga
     9001 aacgatttca aatctccatc aaacttactt gctaatattc tctcgaattc atcttccata
     9061 gaattataac gaacaaaatt cataaaaaat gcattattcg gtttcctctc ccgaaaaaaa
     9121 tcctgaaatt cattcttgtt ctcaaaagtg acagtatcca ggttattaat gattcttgtt
     9181 atcatttcat ctttgtattt tttaatttga gccgtgtctg tctggtttgc aaagttagta
     9241 tacaaactat ctagttttga ggcactggtt agcataaaat ttactatttt ttgctggtcc
     9301 tttaaaccat tttcatactc aataaactga tttgaatctt ttccatactc ggaaatcaag
     9361 aaaagttttg ctgcttttgt gccgatgaaa cttgccagat tctcgttgaa ttcgaccgaa
     9421 tcttttacaa ataatgtgct gtgagtgagt tcatgcaata tcaattcagc cagctcaccc
     9481 tctctcctgt ccagcatgcc ggttaaaaca ggatcactga accagcccag agttgaccat
     9541 gcagttactg ttcttacatt tacatcccaa ttttcttttt ccaattctct tgcttcctga
     9601 attgctctat gcagattgaa atgtccttta tatgttactt caccaagaaa aggaaacttc
     9661 caggtcttgg gctcaagttt gaagggcagg ctcgcagtaa gcacccacaa aatgtcttct
     9721 cctttctgat cgtagactga agtgtaattg tcagtggaat taaattctaa aatattgatc
     9781 gaaaactcct ttacccgggt tactaatcct aacttttgtt taatcgaatc aggtttggaa
     9841 ggatttgcca aatattcatc cagttcgata gaccctttaa taattttcaa ctgaccttgc
     9901 agttggcgaa ttccgtaaat gatttcggaa aaatacccaa cagtcaaaat caggaataag
     9961 attaaaatta ataatgcgct ccgcttaatc cacttcattt atttcgccac aaaagaaatt
    10021 ccggccggta attgagatag aaaccaaatg caaactcaaa tgcgttagta ttcaaatccc
    10081 aatacggctc cacgcgggta gtaaaaataa cattatccat tagataccaa tcgtggaata
    10141 tccgggcaat gatcagcgtc ctccggctca caattagtcc ggcctttttg taactgcttg
    10201 tttcactcgg atataattgc ccgcctttcg gacttgcaaa attgtccgct atccaaaaac
    10261 ttgccattaa gttcaggtta tgtttcaaat tgagccctgc attcgcataa aatccatttc
    10321 cttctccata aaccagcgta tcccctttgc ttgcctccgg atactgagga agtgaaggga
    10381 agacaggccc ggcaggtggc tgtctctggt cattacttgt cttgctgtca ccatttatag
    10441 aattaatata atacgcactg ccattaattg attcaatgaa attaccaaga ggtaaattaa
    10501 gatttacgcc ggcggtcgcg tttactctgg ttaaaagatg attggggctg gagtcgatct
    10561 ggcctccccg gtgataaaca aataattgca gggggatact aatctcaagt tttccgaggt
    10621 taaatttctt atcaattgat aatcctccgg ctatttcttc ctgaacggga tcacctttat
    10681 aaatcgtatt ctcccaattt acccatacct caccaaagaa tttttccctg tcaaccatag
    10741 cctgaaagcc aaactcttgc ctgtcattca aaacccgttc aaaatcataa agcggttcta
    10801 taagtttatg attaagactt ccttccaggt tcccaaaaat caaatgaaag tttttcctgc
    10861 ttaatttaat ggaaaaataa ggttcaatgg cggtgaattt atcatttcca aaatcttttt
    10921 gtaaatagat tcccgcatta actctgactt tactggcggc aaagtatgaa aagtaaggaa
    10981 gaaactgaga accgaaaaga gtatatccat cagcgatata agaactgtat tcattattct
    11041 tcaaaaaatt gagagtctca aatgacaaat agagagagtt tatttttgat gagtcctggg
    11101 gcaaatgatg taagaaagga gtattatcat attgcgccag ggaagggttg atacactgca
    11161 atgcaaagaa accaatgatt attagcgtcc tgaatttaat cataaccaag aggtaaatat
    11221 acataagaac aaacaatgtg aaagcactta aagtattgct tgaacaatcc ttcttgataa
    11281 ttgaaatacc aagtagtaac ttcacatccg gattttagaa aagttaaaat gagtgataaa
    11341 gcagagaaac taaaagccct ccaacttact atagataaac ttgagaaagc ctatggaaag
    11401 ggagccgtga tgaagttaag tgatgaaaaa gtgcttgaca taccggcaat tcctacaggc
    11461 tctcttggct tggatatagc cttgggtatt ggtgggattc caagaggtag agttactgag
    11521 atttacggcc cggagtcttc gggaaagaca acattgacta tgcactgcat tgctgaagct
    11581 caaaaagctg gcggccttgc agcatttatt gatgccgaac atgcgtttga caaatcttac
    11641 gctgaaaagc ttggcattga taccgagaat ctgttgattt cacagcctga taacggtgag
    11701 caggcacttg agattacaga acatttgata cgttcagggg ccattgatat tattgtcatt
    11761 gactctgtcg ccgcgctggt ccccaggggt gaattagaag gagaaatggg agacagtaaa
    11821 atgggtttgc aggcaagatt gatgtctcaa gctcttcgaa aacttaccgg gacaatcagt
    11881 aaaactgggt gtgcatgtgt atttatcaat cagctccgcg agaagattgg agtcatgttt
    11941 ggaaaccctg aaaccactac aggtggtaat gccttgaaat tctattcgtc tgttcgtctt
    12001 gacattcgcc ggattgggca gattaaggaa gccagtaatg acgttaccgg aaacaggacc
    12061 agggttaagg ttgtaaaaaa caaacttgct ccgcctttta aagttgttga atttgatatt
    12121 atttatggtt ctggcatttc caaagccggc gaaatcatag atttgggcgc tgaattggat
    12181 attatccaaa aatccggatc ctggttttct tacaatggaa acaaacttgg acaggggcgg
    12241 gaatctgtga agcaattaat tgaagacaac cccgaattaa tggaagagtt ggaatcaaaa
    12301 attaaaggca aaattaaggg tgaaggagtc aacgagcctg tatcagctta gtctgagtaa
    12361 acaaataata tcttaagttg aattaaaagg atggcgtgcc atcctttttt ttatgatttc
    12421 gaacctaatt catgaaagat tattgcatcc tgatggcttc cactggatct aatctcgctg
    12481 ccaaggcagc aggtacaata ccggctacag ttccaatgat acttgagacg ccaagaccta
    12541 tcgctacatt tctaatagaa agtgaaagtt ccagcgatcc cagggacatg aaagaaagaa
    12601 aataaacgag tagtaatccg aagccaccac cgataacact taagaagata gcttcaaata
    12661 agaactgaaa taaaataaag taattcttgg ctcccaatga tttttgaatt ccaataatgt
    12721 gtgtgcgttc ctttactgat acgaacatta tattcgctat tccaaaacca cccaccagga
    12781 ttgagaaacc tccaattatc cagcctgcta ctgttattat atcaaatgta gagccaataa
    12841 aactttgcag ggcttccggt ctgttaattg cgaaatcatc ttcttccctg ggcttaagac
    12901 ctctcttccg gcgcatcagg cctgttactt cattttcaag ttcaaccagt ccaatatcgg
    12961 tttccctacc cttaatggca atggtggctc ctccgccaaa ccggctgccg ctataataca
    13021 tctttgacca gtctgcataa ggaatataac agttgtcatc attggtgggc aaaccaccca
    13081 ggaatgattc cccttcccgc tcaagaacgc caataacaaa atgatttaac ccacgaattt
    13141 tgatagtttt tccaatgggt tcttcatttg gaaataatgc gtcaacaaga ttagctccta
    13201 ccacacaaac atttctgcca tttatcaatt cctgataggt gaaatacctt cctttttcca
    13261 ccggtacttc atatacatct gagtagcctt gggaaacacc cagaattgtt acagcgttaa
    13321 tgctgttact ttccctctta acagtttgac ctcctcttgc cgaggatatc gtcatcgccg
    13381 aataattgga aagattggta tgaagaaatt cataatcctg ataagtggcc agagggcgac
    13441 gcatgtattt ccaccaaggg tattctcccc ccaacccaaa tggccacttt tcaaccctta
    13501 ttactttatc gcccaagaaa ttgaagctgc tccggatact tgactccagg gaatcaacaa
    13561 cggtaaaaac tgaaataatg gcaaaaatgc caactgtaac accgagaaga gacaatacgg
    13621 ttctaaacag attcatcatg agggcactcc aggcaaacct gaaactctcc acaatcaact
    13681 ttaaaagtac aacactcttc atttaatttt actttattcc cgtattacac taaaaattca
    13741 aaattatcct tacttttgct acccctaaag ttaggctatc cttattattc atttaatacc
    13801 gtaatattaa atatgaaact atccgaattc aaatttgatc tgccttctgg tttaatcgca
    13861 ttatatccgg ctgaaaatcg ggatgaatcc aggttgatgg taatcaaccg taaaaccaaa
    13921 gaaatagaac acaaaacctt taaagacatt gtcagttatt ttgatgagga tgatgtaatg
    13981 gtgattaatg acaccaaagt tttccctgca aggttatacg gcaacaaaga gaaaacaggt
    14041 gcaaaaattg aagtatttct tctcagggag ctaaataaga gcatgcatct ttgggatgtt
    14101 ttagttgatc cggccaggaa gatcagggtt ggcaacaagc tgtattttgg tgaaggtgac
    14161 cttgtggctg aggtaattga caacactact tcacgcggaa gaactatccg gttcttatat
    14221 gatggagaag atgaagactt ctataaggcc atagatgaac tgggagaaac tcctttacct
    14281 aaatatataa agcgaccagc cgaaccatca gatcgggaac ggtatcaaac catcttcgcg
    14341 aaaaatgttg gtgctgttgc cgcacccaac tcgggtctgc atttcacaaa gcaaattatg
    14401 aagagacttg agatcaaggg tgttaaaatt acgcccgtaa ctctgcacat tggattgggt
    14461 actttccgtc ccgtggatgt agaagatctt accaagcaca aaatggattc tgagaatttt
    14521 gaggtaaagc agccaactgt tgacctggtt aatactgccc tggcagacaa gaagcggata
    14581 atagcagtgg gaacaacagc catgagggct ctggagtcat ctgtgtctgc ttcaaacaaa
    14641 ttaaaggaga atgggggttg gacagacaaa tttattttcc caccctatga atttaagatc
    14701 tgtaatgttc ttttaactaa tttccatatg cctgaatcta cgctgcttat gatggcttgt
    14761 gcttttggtg gatataatct tgtgatggat gcttataaat tagccattaa gcagaagtat
    14821 aagttctttg cctacggtga tgcaatgttg ataatataat tccggtatgg ccggcctgaa
    14881 aaaatatgta ctaattgtgg caggcggcta tggaagcaga atgggttctg acgttcctaa
    14941 gcaatttatc tcaatagccg gcaaaccaat tctaatccac acgtttgagg catttctcaa
    15001 atactcccag gatttgattt tcatattgat cctacctgcc gatcaaattg acaattggag
    15061 aaagatctgt gtagaaaatt ctttctcgac accgcatata attaaagaag gcggtgccac
    15121 tcggtttcaa tcggtgaaga aagggctgga aattatcaag gaagatgggt tggttgctat
    15181 tcatgatgga gtaaggccct gcgtgccaca ggaggtaatc agttcttctt ttttaattgc
    15241 aaaatcaaag ggatctgcaa ttgcctctgt ttccatccgt gaatccatcc ggaaggtagt
    15301 tggcaataat tctcaggtaa tgaacaggaa tgattttgtg gtaatacaaa caccccagac
    15361 cttcccaatc aatctaatac aaaaagcgta tgaaattcat gagcaagatt tttttactga
    15421 tgatgccaca gtttatgaaa ctgcaggaat gccagtaaat ttaattgaag gtagttattc
    15481 gaacattaag ataacgacca aggatgattt gcgtattgcc gaagctttct tgacccacaa
    15541 ataaaaaaag catctccaat aatgaagatg cctcacaatc tttttaattt atcaatcagt
    15601 attcgtcttc gttaaaaaag aaatcttctt tagttggata atcgggccaa atttcctcga
    15661 tagattcata aggctggcca tcatcctcta attcctgtaa attctccaca acttcgagag
    15721 gcgctcccga tcggatggaa taatcaatta attcatcttt agtggccggc cagggagcat
    15781 cctccagata ggaagctaat tctaaagtcc agtacatttt tccttcttgt taatagcctt
    15841 attttatcgt gcaaaaatag aatttaatta aacaaatcaa actctaatat cattttttaa
    15901 gaacctgaac caaatattaa ttttcagcct ttcatacggc aaaacctgct ttttgttatc
    15961 tatattgcga cgaaatttta aaagtcctct cacaagcata aatgacccta ttcggatgag
    16021 tgaagaaaag ataatttttt caatgacagg cgtgggcaaa atatatccac cgaaaaagca
    16081 ggtactgaaa gacatttacc tcagcttttt ttatggagct aaaatcggtg ttttgggatt
    16141 gaatggttca ggtaaaagct cactgcttag aataattgca ggagtggaca aggattatca
    16201 tggcgaagtt gtatttagtc cgggatacac tatcggaatg cttgaacagg aaccaaaatt
    16261 ggatccgaaa aaaaacgtca gagaggttgt ggaggagggt gtttcggaag tagtttcttt
    16321 gttaaaggag tttgaatcga ttaacgagaa gtttgcagat cccgcggtga tggagaatcc
    16381 agataaaatg caaaaactaa ttgacaagca ggcggaagtt caggaaaagc ttgacgcggt
    16441 tgacgcgtgg gaactggata accggcttga gaaggcaatg gatgcattaa gaactccatc
    16501 aggagaagaa aatgttgaac atctttcagg aggtgaaaaa agaagagtgg ctttgtgtcg
    16561 actgttgctt caggaacctg atgtcttgct tcttgatgaa cctaccaacc acctggatgc
    16621 cgaatctgta ctttggcttg agcagcactt aaaacaatac aaaggaactg ttattgctgt
    16681 tacacacgat cgttattttc tggataatgc tgcaggatgg atcttagaat tggatcgtgg
    16741 tgaaggaatt ccctggaaag gtaattacac ctcatggtta gagcagaaac agagtcggct
    16801 ggagaaagaa gagaaatcag aatccaaaag aagacgaact ctggagcggg aactggaatg
    16861 ggtacgaatg tctcccaaag cccggcaggc taaaggtaaa gcccgtttga atgcctatga
    16921 aaaattgctc ggagaagaag gaaaacagaa ggaggaaaag cttgagttat tcattccacc
    16981 cgggcaacga ttgggaaata aagttattga agcaaaaaaa gtggcgaaag cctttgacga
    17041 taagttgttg tttgatgatt tgaatttctc actgccacaa ggaggcattg tgggtgttat
    17101 tggcccaaac ggtgccggta aaactacgct ttttaaaatg ataaccggaa gcgtaaaacc
    17161 tgatagcgga tcattcgatc ttggacccac agtgcaaatc ggatatgttg atcaggaaca
    17221 tgcagagatc gaccccaata agacagtttg ggaaaccatc tctgacggga acgatattat
    17281 caaattagga accagggaga tgaactcaag ggcttatgta agtaaattca atttcaccgg
    17341 cccggaccag gaaaagaagg tgggggtatt atccggcggc gaacgaaaca gggtacatct
    17401 tgccttaacc ataaagaaag gagcaaactt gctattgctt gatgagccta caaatgacct
    17461 tgatgtcaat acccttcgcg ctttagaaga tgccctggag aattttgccg gatgtgctgt
    17521 tataattagt cacgaccggt ggttccttga ccgaatttgc acccacatcc tcgctttcga
    17581 aggtgactcc aaagtgcgtt ggtttgaagg taacttcagc gattacgaag aaaataagaa
    17641 aaagcgactt ggtgatattg aaccgcacag aattaagtac cgtaagctaa aataatctcc
    17701 tgttctatcc tccttcttcc ctggcattga aaatcttatt caaccagcct tctctttcct
    17761 ttctttgatt ctccttctcc cattcagaga aatcaaactc tataaattct gacatgtcag
    17821 gctgccctgc atgaacccag caggtatacc aaaaatcacc aaccatttta accgatgccc
    17881 gcatgcgcct ttccaccatc ccggaaagca tctcatgata agcagtggca tattctcttg
    17941 aataggtccg aacgaaagaa tttccccttt cttcatacgt atatttcttg tctgccggaa
    18001 atttattact caattctttc tcaaatcgca aaactgaatc aagagcttga tgaccactaa
    18061 gcactgcctt ccaggcctcc cgcaatggag attctatata ctcagcttta cccacaaaga
    18121 acgtatagtc ctcgaaaaat aattcaggca acctggattc ccaaaaccca tgaattcctc
    18181 tctgattggt taattgtcca ttatagtttt ctgtggtatg aagcggaaca ttggcatcac
    18241 cgatgtaatg tccgatttct gccgagagct ttaggattcg ggcaggatcc ttttgcaaaa
    18301 aagcatcttt tagccgatga tacatagtaa caatatgcca tggaacgatt ccatactcat
    18361 tgagactatc tgccgggatc ttctccgtaa cgcggtacca attctgttgc aaaaattcaa
    18421 tagttgagtc aggataaata tcaaggtcga tataatgcct tggcgcctcc tccttaatgg
    18481 cataacgtcg catatccgga tttacagcat tatcactgag gtatttaatg tgtgccttat
    18541 aaaactggtt taaaggaggc ggaagagtaa aaactgcaag ttggttgatt cgctgatgag
    18601 cgaaaaatcc ccatcgaaaa ctactgatgg cagcactgaa taaaatcaaa aagaaaaccc
    18661 cttttttcat aaatattgat gtgttaaggg gtttaggaat ctatctgaaa gtcagatttt
    18721 gtaaaaatca ataaatactg gtacatttgc aaccttaaaa ttaatttacg ggataactaa
    18781 ttatggctaa ccataaatca gcgctaaaaa gaatacggca gaccgagaag aaaaatactc
    18841 aaaacaaata tgagatgaag tctgcccgta cagcaataaa aaagctaagg acaacaacaa
    18901 aaaaagacga agcggaagaa ttgcttaaga aagtaacctc aatgcttgac aaattagcga
    18961 agaagaactt aattcataaa aataaggctg gaaacaataa atctaaattg aagaagtacg
    19021 tcaacagcct taaataaatt ctaacttaac ttaacaaaaa ctagccctgc cggagaaaat
    19081 ccgcggggtt ttttattgtt ctttattgcc ttatctatgg ataaactgta tgaaaaacct
    19141 tacctgggta ttaagacgtg ggctgaagaa gaccggccca gagaaaagtt gttgttgaaa
    19201 ggcaaaacgc acttaagtga tgcggaactt cttgccattt tgcttggatc aggaagtcga
    19261 gctttaagtg cagttgattt ggccaaactt gttctgaaca gtaccgataa taatctcaat
    19321 ggtctggcca ggctttcagt aaaagatttg atgaaattta aggggatcgg cgaggctaaa
    19381 gcaataagca tagtgagcgc cctggaactg ggaaggagga gaaaagaaca ggagccggag
    19441 aaactggatc aaatttcctg ctcttcggat atttaccgtc ttatgaagcc ggttcttctg
    19501 gatttgaacc acgaacagtt ctggataatt ttactaaacc gcagaaatgc ggtgatgaaa
    19561 aaattccagg taagctccgg cggtgtttcc ggaactgttg cagatcctaa aatgatttat
    19621 aagactgcgc tggagtatct tgcttcatcg gtgatccttg ttcataacca cccgtcagga
    19681 aatctcaagc ccagccagtc agacattgca ttaacccaga agcttaaaaa tgccggtgca
    19741 tttttggatc tgccaattct ggatcacatt attttcactg ataatggcta ttttagtttc
    19801 gcagatcaaa gcatgcttta gatgacccgg aataagattt tctattttgt aattcttatt
    19861 gttgttttat tgattctctt caactccttt gacaatggtg aaaccaggga agaatttctt
    19921 gacaggattg caaccgagag agcaaataag gatctccttt ttaagtcgga taaggaatct
    19981 ccattcaagg attcattggt ctttaccgaa ttgaagtatt atcctcccga tcctgattat
    20041 gtgatcgatg ctaaatttac caaaattgca agtccgcaat tgattacact ggccaccagt
    20101 gatggagcca ccaagaaata caaagaatac ggctacgcgg aatttgagtt agagggtcaa
    20161 atgaacaagc tgcttcttct tgaaatggaa aaaccctttc agaataaatt gtttataccc
    20221 tttgctgatg aaaccagcgc caaccaaacc tacggtgcag gtcgttacct ggaagctgac
    20281 aagcccaatg ggaacattgt cgcacttgat ttcaatcttg cctacaatcc gtattgtgcg
    20341 tattccgaag agtacagttg cccatttcct cctcctgaaa acttgctttc ggtagcaatt
    20401 gaagccggcg aaaagaaata ttacgaaaat taagatttac gctatcaacc aagccgcata
    20461 aatatcttat ttttgagcct ttaaattttc aattccacac caccctgatt catgaggatt
    20521 tcccttaact ggttaaagca atatatcttc ctggaagaaa cccctgaaca gttagcagat
    20581 attttaacta tgaccggact tgaagtagag ggaatagaaa ttttcgaatc cattaaagga
    20641 ggattaaagg gactggttgt gggagaagtt cttacctgtg ttcagcatcc ggaggccgac
    20701 agactttcat taaccactgt ggatatcggt gcgggtaagc ccgtgcaaat tgtttgtgga
    20761 gcgcccaacg tagctaaggg ccaaaaggtc gttgtagcac cggttggaac ggtgatttat
    20821 ccaatcaacg cggaaccatt tgaaatcaaa agaactaaaa tcaggggcga gttttccgaa
    20881 ggaatgattt gtgccgagga tgaaataggt ctgggagctg atcatcaggg tgttattgaa
    20941 atgaaaacag atctgcctaa tgggtctgaa gtcacccatt attttgaaat agaaaatgat
    21001 attgtctttg aaattggctt aacgccgaac cggggcgatg ctacttcaca tatcggagta
    21061 gctcgtgatt taaaagcttt tttgagaaga aagttggaat ggccatctgt agatcaattt
    21121 aaggttgaaa gaaatattat gaatatttct gtagaaataa gagataaaaa tgcatgtccg
    21181 agatattcag gcatatcaat aacaggaata aacgttcaga cgtcgccacg gtggcttcaa
    21241 aacaaacttg aatcgattgg cctttccccc ataaataaca ttgttgatat taccaattat
    21301 gtgttgaatg aaaccggtca gccactccat gcttttgatg cggataagat tgcaaatggc
    21361 agggtcatcg tacaaactct tgccaatggc actaaattca caacgttgga tgaagtagag
    21421 cgaaccttat cagcagataa cctgatgatt tgcgattcaa atggcggaat gtgtattgcg
    21481 ggcgtttttg gcggggcagg ttccggaatt acaaaagaga ctgtaaatat tttccttgaa
    21541 tccgcctact tttccccgga ttctatccgc aaaacaagtc agcagcatgg cttgaaaaca
    21601 gatgcagcct tcaggttcga gcggggaact gatcccaatc ttaccgttta tgcattgaag
    21661 cgggcaacca tgcttatcaa ggaaattgcc ggagggcaga tttcctctga aatagtagat
    21721 gaatatccaa gccaaattcc agaccgcgaa gttgaaataa cttataaaaa tgtcgacagg
    21781 ctgatcggaa taactattga tcgtgaaaat attttctcaa ttcttgaaag ccttgatatc
    21841 aaaattgaaa ataaaaccca cgatggattt accgctattg ttcctcccta caggtatgac
    21901 gtaacccgtg aagccgatgt tatcgaggaa atccttcgaa tttatggact caataatatt
    21961 aaaatacctc ccaaagtaag cgctgaatct ctggcggaat ttccccaaaa agatcccatt
    22021 aatattcttt cccgaaccag taaattactg gcagcaaatg gtttctatga aatcctgacc
    22081 aattcattaa ccaagccgga ttatgctgaa tcagtagatg aaattgatac ctccgccaat
    22141 gtggctataa taaataagct aagtgaagac cttggagtaa tgcgtcaaag tttgcttttc
    22201 tcgggactgg aagtaatggc ctataatatt aacaggaggc aaacagattt gaaatttttt
    22261 gaatttggaa agatttatca caagaaagcc gggggttaca aggagaagca atgtattgga
    22321 ttgttcatta ccgggctacg agagaaagaa aactggatcc gcaaagattc cccggtaatc
    22381 ttccatgata ttattggcgc atcgcagctg gttgttgatt cattatgtgg cgaaatcata
    22441 actcaatctc catcccgaca aagtattttc tcctttggat gttcgcttaa ggttggagac
    22501 agagatattg gaatagcagg taggattaat ccaagaattt taaaattgtt tgatatttct
    22561 aaagaaatat tttactcaga attggattgg gacttgcttt taaaaatctc aaatgataat
    22621 atcgtgtacg aagaagtgac ccggtttccg gaagtgcgaa gagacttatc tttggtttta
    22681 gataaagaag tttcattcga agaaatccgg aggctggctc agaagcagga gaccttctta
    22741 attaaagaaa tgaatgtttt cgatttatat gaaggagaaa acattggaaa aaataagaaa
    22801 gctctggcca taagcttcat tcttcaggat aagaagaaaa ctttgactga taaagtcatt
    22861 gataaaacca tgaaaaggtt aatgaatacg tttgaaactg aattaggtgc tataattcga
    22921 aaataatgag ccaagaaaga ctgcaaaatc aattattctc cctggagcgc aaagtaaggc
    22981 ttctggtagg ggagtataag aagctaaaag aagagataat tcaggtaaaa ggagaaaatg
    23041 acaccttagg ccggactata ctgaaaaaag aacaacagat aggagatttt caaaataaga
    23101 ttaatattag taaaattgtg gattcgatag cagttgatga tgtaaaagct gctgagttga
    23161 agaagaatat tgatttgtat attaaagaga tcgataagtg catatcgcac ttgagtagat
    23221 aaaataatcc gaatggagca actttccgtt aaaataaaaa ttgccgacag ggaatatcct
    23281 atgaaggttc gacccgaaga tgaagaaaaa gtgcggaatg ccgggaaagt cattaacgaa
    23341 agattgaaga aatataaaga gcaatttgga ttagaagata aacaagattt actggctatg
    23401 gtagcttttg atagcctggt ggatttacta aacctggaaa acaacatttc gggagccgac
    23461 gatggtgttt tgcagaaagt cgatgaaata aaccagcttg tatctcaggt tctttaaata
    23521 atactccaca tcagtatagc ccttcacctg gcttttatac atcccgacta tcgtcaaaaa
    23581 taaacgtgac gggattatgt ctgaaatcat gataattatt gcctccgccg gtgcccttgt
    23641 agttggtatc ggacttgggt ggttgatttt cagtaagaaa tttcaacagg aggaaaaaaa
    23701 agcattagaa aaagccgaaa ttattatcag ggaagctgaa attaatgcag aaacgcttaa
    23761 gaataataga attctggaag cgaaggaaaa gtttctcaaa atgaaagaag attttgagga
    23821 agaagcccga aaaaggaaga acctcattct tagcaatgaa tccaaaatca aacaacggga
    23881 ggcaagtatt tcaaagcagt ttgaggatat gaaacgaaaa gaaggagatt tggatcgtca
    23941 aaaggatgaa cttaatgaac agctggatat cgttaaaaga aggaaagatg aacttgacaa
    24001 attccatcag cagaaagtga gtattcttga aaagatttca aacctgactg ccgatcaggc
    24061 gagagaacaa ttgatggaat cgcttgtgga agaaactaaa gcaaaagcct cttcaaaaat
    24121 taaagagatt gtagacgaag caaaactaac tgctaccaag caggcgaaaa aacttgtcat
    24181 tgaaactatc caacgaacag ccacagagca tgccattgaa aattgcgttt ctgttttcaa
    24241 cattgatagc gatgacatta agggaaaaat aattggccgc gaaggtagaa atatccgggc
    24301 cattgaagct gccacgggag ttgagattat cgtggatgat actcctgaag caataataat
    24361 ttcaggcttt gatcctgtta ggcgcgaaat cgcgagatta tccctgcaca ggctggttgc
    24421 ggacggaaga attcacccgg caagaattga agagatcgtt gctaaaaccc agaagaatat
    24481 tgaagaggag atagtggaaa ttggggaacg caccgtaatt gacctgggga ttcatggatt
    24541 gcatcctgaa ttaatcaaaa tggttggcag aatgagattc cgatcgtctt atggacaaaa
    24601 tttgctgcaa cattccaggg aagttgcgaa cctgtgtgca ataatggcaa ccgagcttgg
    24661 tttgaatccc aagcaggcga agagagcagg cttgcttcat gatattggaa aggtttggcc
    24721 ggaagaatcg gaaaaacctc atgcaattct gggaatggag attgcccaaa aatataatga
    24781 acaccctgct atttgtaatg caattggagc tcaccatgac gagatcgaaa tgaccaccct
    24841 ggtttctcct atcatacaag cttgtgatgc catttcgggt tcaaggccag gtgcccgaag
    24901 ggaggtcatg gaatcttata taaagagact aaaagatttg gaagcacttg cattaagctt
    24961 tgatggtgta cataaatgct atgccattca ggccggaaga gagttgaggg ttttagttga
    25021 tgcggaaaac gtaaatgatc aaaaagcgga aatgctttct tttgaaataa gtcaaaaaat
    25081 tgaaaatgaa atgcagtatc ccgggcagat aaaggtgaca gtgatccggg agaaacgggc
    25141 agtgtcttat gcgaagtagg atttcgtttt agcccgctta gtgattaatg ttattcaata
    25201 attcctaatg aaaaatattt aggaccagat tcattctaat ctttatatga tttttcatag
    25261 gtataaagct gattttgatc aataatggct gtttttgatg ggatgtttaa aagatccaag
    25321 ttttaagttc aacctaatta tttatcgctt ttctgaatac agagaggtta cagttatctt
    25381 gacagtcgta ttgataattg tattcaaaaa aaataactaa ttacaatatt ttgttatttt
    25441 ggcattttat taaatatttt atttccaaac taaatcctca atccatgaaa agagtgaatt
    25501 tacttgttaa aactgcaatt gtgtttggac tcgtatggct ggcatcatgc aaagctgatg
    25561 acattcttaa tacctgcaca acttgtactg aaacaacgac acaagtttct tcagaatatt
    25621 gcggtaccat tgttgaagtc caaacttttg agcagacact ttacaatact tccggacagg
    25681 actggacctg tgtccgcaat tagaagttac ttttatgtaa ttaaagccgg caaatgccgg
    25741 tttttttatt tatggccatt ccgactctaa cattttctgt taattcccgt attttagctg
    25801 attagtttcc ccggaccttc atgcggctaa ttcctttgac agcctttatc gttttaatgc
    25861 tgattaattc catcccttta cgcggacagg cctggcttga tttatcacat catgattttt
    25921 tgacaaaccc ggaaacaaaa ctatcaggtg attgggagtt ttattggaat caattgttag
    25981 agccgggtga ttttaatccg tctttaagac ctgacttcat tcatgttccc ggcgtgtgga
    26041 acaggcaggg agattacaaa acacttggct atggaacata ccggtttcaa attaaacttc
    26101 cgcccaatca atttggattg tcggtttact ttccaataat aaattcttcc gctaaaatat
    26161 gggtgaatga tattctggtt gccgaaacag ggatcgttaa ttcagacaaa aattattata
    26221 cgccgcagct aatgggaacc gctgttcctt tgccggataa agcggaagtg gttaccctgg
    26281 tggtacaggt agctaacttt acctattata caagcgggat tccatcccct ccgagagttg
    26341 gacgatcaag tgccattttc gacagaaata atcgcaataa cggcattgaa aatttttttt
    26401 ccggcagttt aattgccatg tgcatttatc aactgatgct cttttttctc aatacccatc
    26461 gaaagccata cctctttctg gcattgattt gttttggtgt tgccatcagg gcattgatag
    26521 tccacggtgg ctcattcctt ttgccgaatc tatatccttc cgtgcattgg gaagtatgga
    26581 aaaaaattga gttcgccagc gtttatggaa tgctaatatt atttccatct tacatttatt
    26641 atcttttccc aaccgctgcc tcaaagcgta ttcaacagac attcctggtt acgggcatta
    26701 tactttgcat accggtaatc tttacacccc attatgtata tggtatgctg ctggaaatcg
    26761 tacacctttt gttgttgttt gcttttgtct atgccattta ttcggttata aaagcatggc
    26821 gtagtggaaa taaagatgca cggataattt gctttggcat tattgcttcc tttcctttta
    26881 ttctgattga aattattcag aactcggttt tcactcaata caatattgaa ttcgaatttc
    26941 tggtggaggc aggtgtactg gtattcctac tatttcaggt ataccttctt ggccggcatt
    27001 atgcacaggc ttaccggaga ctggaagttt caaacaggga actggagaga atggtttcaa
    27061 acagaacaat agaactggta acttctaact cagttaaaga ccggctgctg tcggttatgt
    27121 ctcatgatgt aaagagtcct ttaaattcct tacaggggat tttaaatata tataacaagg
    27181 gcgcgttgca gcgagaagag tttgataatt tcacaaaaat tattgaagct gatttgcata
    27241 agacaagttt attggttgaa aacattttat tctggaccaa aaatcagatt aaaggcgtag
    27301 aaattaaaat cgaaaaattt gatcttaggc agttactgga tgaaattaca gggctctatc
    27361 agacaattgc cggtaataaa ggcatccaac ttggtcataa cttaacaaat gaaaccgtca
    27421 taaattcaga cagaaatatt ctctacttcg taatccgtaa tctggtagct aatgccatta
    27481 aattttccag ggagaatact tccgtaagag tagaagttga ttatgagaat gatactattt
    27541 tgattagggt taaagatcaa ggagttggaa tgaatgatga aattctcagg aatctatgcg
    27601 ccccggaatt aattctaagt acaactggta ccgaaaatga aaaagggaca ggactcggca
    27661 taggtctgtg taaggagtat cttgaaaagg ttaatggtga aattgtagtg gacagccttg
    27721 aaggaaaagg cagtactttt acaattatta tcaataagaa gaattaacca aataattttt
    27781 tgaaaaatcc ttttttagaa actttctcat gcagtaaatc aagtgtagtt cttagtctta
    27841 ccaaagaagc ggagttctta ataatatatg cctcggcacc taagtcaagt acctcttttc
    27901 ttaccttgtc gtcatccaat gttgtgtagt aaatgacagg agtcttattc cttattgatc
    27961 ggagttctct caaatattcg acaccggtat tttgactttt ctcaccaaaa aaatgatcca
    28021 acacgattgc aaacggctta aggtggatat ttgacatcat atcctgggga gtatcaaatg
    28081 tctttactac gtaattccca aggctctgct caaaatgttc cctgaggata cgttgctggg
    28141 cagggttgtc ttcaacaata aatactaaat tcggcatcgt ggatgggtta atcagggtaa
    28201 attaatcaat tttacaacat gatgtaatat caaatcataa attgagaaca aaatcaggtt
    28261 gacgcttgtt aaacttttta aaaagtaatt caaatggaca gtacctcaat aaagaatata
    28321 aaacctttgg gatttccttg ggaaacccag gatccttttc tgttttgtgt gcaccatgag
    28381 gatttttatc ctgaagggaa tgaacaaatg ggaccaaaag cttccctggc gggccgaaat
    28441 atagggcagg attttaccat taaagatggt tggagaatgt accacggtca gaacgttccg
    28501 ggcttccctg cccatcctca ccgcggattt gaaacagtaa ccatcgccac caagggacat
    28561 attgatcatt ccgattcttt gggcgccgcg ggcaggtttg gcaacggtga tgtacaatgg
    28621 atgactgcgg gcagaggtgt tcagcattgc gaaatgtttc ccttgcttcg ggaagacgat
    28681 aagaacacca tggaactctt tcagatttgg cttaatctgc caaaggccaa aaagttaact
    28741 gatccctatt tcagcatgct ttggaacgaa gacatcccaa actataatca caaagattcg
    28801 aacggattgg aaacccgggt gaaagttgtg gcaggaaaaa tagcaggcgt gcaggctccc
    28861 ccacccactc cggatagctg ggcagccgct ttgaataacg aggttgcaat ttggttgatc
    28921 cgcatgaata ccggagcaaa atttatcttg ccacccgcct cagccgcagt aaaccgaacg
    28981 atttatttct actctggtaa aactgtgaat atcgctggac aggaattcga ttcctacaac
    29041 gcaatagaat tatttggtga caaggaagtt gaaattttga atgggaatgt agagtcttgg
    29101 ttcctgctac ttcagggaaa gccaatcggt gagccggtgg ttcagtatgg tccgtttgta
    29161 atgaatacgc aagctgaaat tcagcaagca ttccaggagt atcagcaaac acaatttgga
    29221 ggttggccct ggcctggcaa tgaacacgtt catgccaggg aaaaaggaag attcgccaaa
    29281 tacccggatg gcaaaattgt tgaacgatga atgttcgttc gtgaacaatc aattgcgcgg
    29341 aattaattgt ttcttgcagc caaaaattaa cccaggacgg aaactcaaag gttttacaaa
    29401 ctggtttgga atttgcacgg gagattttaa actttattta gatgcggatt tttattgcac
    29461 ttgtgttatg gtggattctc ttcatcattt gctggcctat tgccattctt ttgattttac
    29521 tttatccaat catttggtta ttacttcttc catttagaat acttggattt gcagtggaag
    29581 gtatttttaa actgatcagg gaaatatttt tattgccttt caaggtagtt agaagtattg
    29641 cacctcgata gtccggtagt cattaagcga actgaattcc aaaaattact accttcgcat
    29701 taatggcttc tgtaagtaaa tcaaaatttg cattattgca aaaagagcta acatttacat
    29761 cagcccggtc aagtggtcca ggggggcaaa acgtaaacaa agtcaataca aaagttactc
    29821 ttagattcag cattcaaaat tctgaaattc tttctgagga agaaagagga attcttttga
    29881 gaaaattggc aaacaaaata actaaagatg ggagtttgat tctttcttgc caggaaaagc
    29941 ggtctcaggt tcaaaataaa gaattggtta ttcaaaagtt tcttgattta attgataaaa
    30001 ctcttaagaa gcccaaagca agaaaatcta caaaaccatc gaaaacgtcc agagaaaagc
    30061 gacttcaaaa gaaaaaggaa atttcagaaa agaaaaagtg gcgcggtaag atatagaagc
    30121 gctgagggtt tatcagccaa ttcttttata attagcaatt atttgcctaa aatggatgtt
    30181 tccaaaccac tcaaactgaa attactctga ccgctttaag caagaaatta aaaaacattg
    30241 gccccggtgc aattgtggcg gcggctttca ttggacctgg tactgttact acagctacat
    30301 tcgcaggcag cagttatgga tacaccctgc tctgggcaat cgccttttct atttttgcca
    30361 cctttattct tcaggagatg gcatcaagac ttggaatcgt tggtcgtatc ggcgttgggc
    30421 aagccatccg tcaaaaagta atgcaacctt ttattaagtg gggagcttat tttcttgtta
    30481 tcggcgcaat cgtaattggc aacgccgcct acgaagccgg taatattacc ggcgccgcat
    30541 tgggatttca gaaatatgga gaatctattt cagtcctggg aataaacccg cttgtaatca
    30601 tcattggcat cctcgcattt tcattattgt tcagcggaaa atataaagta attgaaagat
    30661 cattaattct gatggtaagt attatgggaa tcgttttttt ggcatcggca gttttcctgc
    30721 agccaaacct aagggaagtg ctgaaaggtt tattcacacc gttgttgccc gagggatccc
    30781 tgatgatggt gattggattg attggaacca cggtggtgcc ctataatctt tttttacatg
    30841 cttcaacagt aaaagaaaaa tggaaaagcg cctcagatct taaaacttct tactgggata
    30901 cgctaatctc cgttaccgct ggtggtatta ttacgatggc tattctgata acctctgcca
    30961 tagcttttga gggtgaagct aaagcgactt caatcgctga tctttcctac cagctcactc
    31021 ccctattggg taattggtct gaatatttta ttgccgtggg ctttcttgca gccggcttat
    31081 catcctcaat aactgctccg ctggccgccg cttttgcaac atcagagatt ttgggttgga
    31141 acagcacaat gaaaggaaag aattttcgca gcatttggat ctttgtactg gtaacgggta
    31201 ttgtgttttc ttcactgggt ttcaatccaa cctctgttat tctttttgcc caggttgcca
    31261 atggaatgct tcttccgata attgccattt tcctggtttg ggtgatgaat gataaaagga
    31321 taattggtga gtatgtcaat tcaaaagtta ttaatttcct gggaatcctt gtgattattg
    31381 ttacccttat cctgggtttg aaaggaattt tatctgctac taatttcttg taactttaac
    31441 ctgattgaaa aatttagaaa tcgatattaa ttgcgacatg ggtgagagct ttggccaatt
    31501 taaaattggc aatgatgaag gaatttttcc ccatatcacc tcttgtagta tcgcctgcgg
    31561 gtttcacggc ggcgatcctt taaccattga gaatactatt attggcgcac tgaaaaatgg
    31621 tgttaatgta ggcgcgcatc cctcctatcc cgatgtgccc ggttttggca gaagaaaaat
    31681 ggatttacaa aaagcggaac tcaaagcgct tatcaagtat caaataatag cggttaaagg
    31741 tctagctgaa tgcctgggag ccgcgattac ctatgtaaag ccgcatggtg ctttgtacaa
    31801 ttccatggta attaataatg aggaaaccaa atgggtgatt gaaggaatta aggaaataga
    31861 caatcaactt atgctgatgg gactggcggg gagtcaggtg gaaagcattt caaaggaaat
    31921 gaacattgaa tttatcgcgg aagctttcgc agaccgtcgt tacgagccgg atggaacgtt
    31981 gatgtcgcgg cgcaagaaaa attccattat cagcgatccg aaccaggccg ctgatcaggt
    32041 tatttccata ataaaaaata attcggttga aagtttggat ggaactttgg ttcctatcaa
    32101 agcgagaagc atttgcatcc acggtgacaa tccaaaggca gttgaaattc ttaccgcgat
    32161 tacaatcaaa ctcgctgaaa ataatatcat taaaaaaagt tttgcatccc gatgaccatc
    32221 attccctatg gtgattctgc attgctaatt aattttgaac aggttattga tccccaggtg
    32281 aatgaaaatg ttattgaact ggccaaagtt atagagcatt ccaatattgc gggggtaact
    32341 tttcttgtcc cggcttattg ttctcttgca attggatttc aacctgaacg aattaccttt
    32401 caacagttga agaaattaac ggagaacctc aattctaata tatctaaaca tcaacatcct
    32461 gaatcaaaaa aaatatccat tccaatatgt tatgagagcg aatttgcttt agataagcag
    32521 gaggtaatga gttatactaa cctttcatgg gatgaaatta aacaactcca tttatctatt
    32581 tcctaccggg tgtacatgct tgggtttctt ccaggttttg tttacatggg tagtcttcct
    32641 gaaaaattag aatcaccccg aaaaaaaatc ccaagattaa aagttccggc attatccgtc
    32701 ggccttgccg gatttcaaac cggcatttat cctttagatg ctcccggcgg ctggcaaatt
    32761 atcggtaaaa ctccaattcg aatttttgat aatcgcaata ggaatccttt tctctttgaa
    32821 cctggaatgc ttgtaacatt taaagaaatc acggaggatg agttttatta tatggcgacg
    32881 gaggttgaca acaggaattt tgatttgagt tccctttatg gatagtatta ttactttaag
    32941 cataataagg ccagggttaa attctatggt gcaggatgcc ggccggtcgg gttatcaaaa
    33001 gtttggagtt cccgtaaacg gatttatgga tagatcatca gcacgaatag ccaattggct
    33061 tgttggaaat cctttaaatg accctctcct tgagattaca atgctgggac ctcaaattga
    33121 aattgacaat tcctgttcca ttgcggtaac cggagcagat atttcaccca tgttgaatgg
    33181 agaattaatt cctatgtatg aaacacaccg cgttgctagt aattctacgt tgtcattcgg
    33241 caagctaaat tcaggttgcc ggacatatat ttctttcagc ggaaaattgg acgtaaagca
    33301 atggcttggt agtgccagcg cctcagcaca cgaaactcaa aatctgaccc cacaaagtct
    33361 gttaactaaa aacatgaata taaggttgat ctccagcggg agtctaattg aaagaagatt
    33421 cccatggaaa tcaaaatttg aaagtaaaat tgtagcgaga atcttaccgg gacccgaata
    33481 caactacttt tccggagaaa cactttctca tttcctcaac catgaatttg ttgttggaat
    33541 aaattccaac agaatgggct acaggcttga aggaactgtc aggggattca accctgataa
    33601 ggaagtaatt tcttcagcag ttattccggg aacaattcag ataaccaatt ccggatcacc
    33661 cataattctg atggctgatg cccaaacctc cggtggatat ccgcgccttg ccaatatcat
    33721 ttcagcagat ttagataaag tagctcaatt gaaaccagga gacactattg catttcaact
    33781 tacaacgctt gaaaaagctc tggaggccaa aaaggtctac gagcattacc tgaatcaatt
    33841 gaaataagtt ttttttgaaa ataatcccga atacctgaaa cctgaccagc taaaggagtg
    33901 tctatataac ttatattcag ttgctttctg ggggagttac agttcaataa aaaaaaaatt
    33961 ctaactcatg attcaaaact atttcaaaat tgcctggcga caaataatcc ggcataaggg
    34021 atttagcata atcaatatca ttggcttgtc aattagcctg gccgttggtc tggtgatcat
    34081 aatgatggta accgaccaac tcctttatga tacttataac cgcaataagg aacgggttta
    34141 tcgaatcaat acagaggttt taagaaagaa tgaagtttct tcgttgaata aatacgccac
    34201 aacttcatta cccgtcgggc atcagctggc tgaaaattac accggcatcg taaaatcagc
    34261 cagattatta agaggctttg gtaacggatg ggttgaattt gatccggaca acgatctaaa
    34321 cattccgctc ggtggtttct ttgccgatcc tgagattatc gaaattcttg atttagaatt
    34381 tgaatttgga aatccggtaa cagcccttgt aaatccatat tcagtagtaa ttacacgaca
    34441 ggcagctaat aaacttttta aagagaagaa tccggttggt gagctaatta aagttggtga
    34501 actaggcgaa tacaaagtca cgggtgtttt gaagaatacc caaagcaaat ctcatgttat
    34561 ttttgaagcg ttggcttcca tttccaccac accaactttg atcgctgaag aaaaattaag
    34621 accagacaga gattcctgga agaactgtac cgccgggtgg gtttatgttt tgcttactga
    34681 aaacaaaaat ccacaggaag ttgatgttta tttgcgggaa atatcccaaa aatttggaag
    34741 cgaaaatgat gaccggaatt tcaggtacta tttacaaagc attacagaaa ttaacccggg
    34801 gccattcctg gccaatcaga tcggacccgg aatgccaatg ataatggttt actttttagg
    34861 aggcctggcg ctgatagtaa ttttaacagc ttgttttaat tatacaaatc tctctattgc
    34921 gcgggcatta aacagggcaa aagaagttgg aatcagaaag gtatccggtg ccaaacgaag
    34981 acagattatc tggcaatttc tcggagaatc aattttgatc tcattatttg cattatccct
    35041 tgcgttcatc ctcctgattt tcattcaacc cgctttcaga aacctgagtt tttcaagaat
    35101 cctgcaatgg gatttaacta ttaatctccc ggccatcttt gcctcagtaa ttgtcgcgat
    35161 tacagtgggc attctcgccg gtattttacc cgcaatgttg ctttcatcat ttcagcctat
    35221 aaaggccctc aaaaatctca gcgggatgaa actactctca cgggtaggac ttcgtaaagg
    35281 attgctggca gttcaatttg gtttatctat aattttcatt atttcggttc atttgattta
    35341 taaccagatg aattacatga tgaatgctga ctatggattc aactatgaaa acaatgtccg
    35401 tctggcctta ggtgaatctt cctggagaaa tatgaaatct gaacttctga gttatagtga
    35461 cattgaaaat gtttctgttg ttagtcatat tccggcggca ggcacgactt atggtgacac
    35521 attcttcaaa gaactcaatg acccgaggtc aatggattat ttcagcgtgg atcaggattt
    35581 tattgaaaat ctggaattac agcttttggc aggccgaaac tttccggaac aactttctga
    35641 gggcgaaaca atgattatca tcaatgaaaa agcagttgat gagtttggat actcctcacc
    35701 acttgatgct attgggcaaa ccctttacaa tgcggataca gtagcggttc agattattgg
    35761 cgtggtaaaa aatttccatc acgccctgat gatgcagcag attgaaccaa tggccttacg
    35821 ttacaggcct caacaattca aaattattca ggtgaaaatg gctaacccgg aagcaattgc
    35881 cagcgtggta gctgcctggg aaaaagttaa tcccggcaag cttattaatt acaaactgat
    35941 gggcgatgaa ataaaagaat tgtatcaatt ggttttcgga ggattggcaa aaataatttc
    36001 tttcatctca ttccttgcga ttgttatttc gtgccttggt ttgttgggta tggcaacata
    36061 caccattgaa acccggctca aggaaatatc aattcgtaaa gttcttggat caagcgattg
    36121 ggcctccatt tacttgttat cgaaaggctt tatcgcatta cttgggatta caattgtctt
    36181 tgcggttcct cttgcaattt tgattaacaa tttatggctt cagttgatcg cattcagagt
    36241 tggcattggt gcaagtgtag ttgtgaccag catcttgata ataatgattt tgggtttatt
    36301 tactattggt tctcaaacat tgcgcgcatt gaaagtaaac cccgctgaaa ttctcagaaa
    36361 tgaataactg agtgcgttaa aaaatcggcc tgattgtact ttacaaataa ttttatggtg
    36421 taattccggt tgtcttctct attcggctcc tggattttct acctgaagta ataccggtta
    36481 ttaccgtgat tctgccggtt tatcaattaa ctttgatgcc ttatccacca aacgttttta
    36541 tattatgatt aaaaagattc ctttgcaacc tatttgtaaa aagtaagtac ttattaagat
    36601 cctaattgaa ttactaatgt tgaaacatca tctattgctt gcatttcgca atttcctgcg
    36661 ttataaaagt tctttcctca ttaatctggt tggattatcc accggtctcg cctgcgtcct
    36721 cttaatttac ctctgggtag atgatgaact tcacatggat aaattccatg agaaagatgc
    36781 cagtctttac caggccatgg aacatcagaa gtacgcggaa gaaattatga ccaccacctc
    36841 tacacccggt atcctggcgg aagcactggc ggaagagatt cccgaaattg aatatgccgc
    36901 taccgcaaca tgggtaagtg attttaccct atcaattgga gaaaaaaatg ttggtgctaa
    36961 aggatattac gttggcaaag attatttcaa tattttttca cttggcctga tccacggcga
    37021 tgccaatcac gtgttgctgg ataagaattc catggtaatt tcatccgagc tttcggcaaa
    37081 actttttgaa acaactgagg gtgttattgg taaagaagtt cagcttcagc atgacaaaac
    37141 ttttcttgtc tccggagttt ttgaaggtac tcccaggcag tcctccattc aattcgattt
    37201 tgttttatct attgaacttt tcaaagatga aaaccaatgg gcgaattcct ggggtaataa
    37261 tggtcccagc acttttgcag tgttgattga cggtgcagat gttgatgaag taaattataa
    37321 aattgaagac tttatccaaa aaaagaatga acaatccaat gttcgattat tcctgatgcc
    37381 ttactctgaa aggtatttgt acgggcgatt cagggatgga aaaccggctg gtggacgaat
    37441 cgaatatgtc agattattct ctataatcgc cattttcatt ctggtaattg catgcattaa
    37501 ttttatgaat ttatctacgg caagggcaac ccggagagca aaggaagttg gaatcaagaa
    37561 agcggttggt gcttataaag gaacacttgt cagtcagttc ttaaccgaat caatcttaat
    37621 tacttttatt tcattattaa ctgccatagt aattgttaaa ttgattctgc cccagtttaa
    37681 tataatcaca gaaaaacaaa tagaactaat ctttactccc actttgatac tttgggcgtt
    37741 ggggatcacc atatttacag gattagtatc aggcagctac cccgcttttt atctctccgc
    37801 ttttaaaccg gtaagggttc ttaaaggaga tgttaagggt acaataggcg aactatgggc
    37861 aagacgcggc ctggtaattt ttcaattcgc tatgtctaca attctgatat tttgtgttct
    37921 ggttgtttac aatcagattc aatatgttca gaataaaaat ctgggatata ataaagacaa
    37981 tattattttt ttccagatgg aaggaagagt ggaatcgaac cgggaaactt ttttatctga
    38041 agtcagaaaa cttccgaata ttgtttcagc agcaaccatc ggacacaatc ttttaggcag
    38101 gcaaaataat acctcgggat tggaatggga tggatataac ccggaagaga aaatattgtt
    38161 tgaaaatatg cgggtgggat atgacttgat tgaaactttg gggatagaaa tgaaggaagg
    38221 ccgtagtttt tccagagatt ttgccactga ccccattaag attattctga atgaagccgc
    38281 tatccgcact atgaattacg aaaatcctgt gggcagaacc atgaaattat ggggaaaaga
    38341 acgtgaaatt atcggagtgg tcaaagattt tcattatcaa tcgcttcata gtgaagttaa
    38401 ccctttgttc ttcattttgg ccccggaggt tacctggtat ataatggttc ggattgaagc
    38461 aggaactgaa aaaacaactt taaatcaact ggccggtttc tataacgaat ttaatcctgg
    38521 tttcaccttt gattatgaat ttttggatca atcctatgcg ggaatgtata tcgctgaaca
    38581 acgggttgct tcattgagtc aatattttgc tggttttgct attctgattt catgtcttgg
    38641 attgtttgga cttgcagctt tcactgctca aagaagactg aaagaaattg gaatccggaa
    38701 agcgatgggt tcaagtgtca gcagtatagt gcttttacta tcaaaggact ttacgcaact
    38761 ggtaggcatt tctatagtgg tagcattgcc ggtgagttac ttcctaatga gcatgtggct
    38821 aagcagattt gcctttaaaa tcgagcttag ctggatctat tttgtgggtt caggacttat
    38881 tgccatttta atcagctggc tcaccattag cattcaatct ttcaaggccg cgagggttag
    38941 tccgacacag tgcctgagga atgaataaaa acttcaaccc gctgactagt ttcaattcaa
    39001 agctggcagt aattccgggg attagagcaa aaccttgatc agagtggcca ggatcatcaa
    39061 taccagcagc cttcttatcc agaacccagc attagttgaa acattggaga aaattcccgc
    39121 ggcaattgct ccgataattt gtcctactgt aagcgccagc gcgggaatcc aaataatctt
    39181 tccctggtaa ataaaataaa tggtggttgg aaccgtataa accagtatga ttatcagttt
    39241 aaggaaatta gattccacca aatccgaatc gctcaccaaa tggattgcaa atacaaccat
    39301 aattccaatt ccaatttgaa taaacccgcc ataaagtcca ataataaaga ataacaagac
    39361 tgaaagccat gcagtatgag tattaaacca atgcaaattc ttgatatact ttttgaagtc
    39421 gaatattgcg tcgaatagta agattaccat agctattccc gccacgatat cgagaaatct
    39481 ttcagacaca tttatagcag caaaggctcc tatcattgct ccggcgagtg ttggaattac
    39541 atattgcagc tttaaattgc gaagtgagta ttttccgtaa cttcggaatg taattattgc
    39601 agtgacattt tgaaagaaaa cgccaattct gttggtggta tttgccagat caataggaac
    39661 gccaagatat aacaaagctc caaaggaaaa gatggtacca ctgcctgcca gggtattaac
    39721 aattgctgtt attatccccg cgagaacata aatagttatt tcaaatgcgg aaatcccttc
    39781 caaattatct taatgacaat actatttaaa ctccgttaaa tatagcaatt gttcaattgg
    39841 attgattatt cataaaaagt tccgggtgag attcttcaaa atccgttgcc ttttgaaaaa
    39901 tatacgcggc ttcaaggatt ttcccatcat caaataaatt accaattaaa gtaactgaag
    39961 tgggattacc cttttcatca aatccatccg gaactgatat ggcaggatgt ccggttaaat
    40021 tcgtaattat agattgcctg ctcctgaaag ttggtgcaat tataaaatca tattccttga
    40081 ttacttcatg aaatgcttcc atcaatttct ttcgatgccg gttcgcctga agatattcca
    40141 cggcaggaat aaaccgggat tgccgaaggg aattggcccg tgatcccctg tcctgttcaa
    40201 ccattgtcct gtcttgatgc gacaaaacca gttcatcgaa aaatgcacct gcttcagccc
    40261 gtaggattat atcaaatacc tggtaaggct ggccatcagg aagtttaact tcttcaagat
    40321 ttgctcccaa tagtttcaaa gtttccaggg ttctgtcttt attagaggaa acatcgctgc
    40381 tgtcacgttc aaaatcttct ttaaggtatc caattttgta accgccgaga tcggtggccg
    40441 gattaaatat taatgtaaat tgccgggtag ttctatccct tatatcttca cctttaatcg
    40501 cctttaagac aatagcacaa tcaagagcgt gtctgcaaat tggacctgcc ttatccatac
    40561 tccaggataa agccatgcag ccgtaccggc ttactgcacc aaaagttggc cgcaaacctg
    40621 tggcaccaca ccgggaagaa ggtgaaataa tggaacctaa tgtttcggta ccaatggcaa
    40681 aacccacaag accagccgca acagctgctc ccgctcccgc tgaagaaccg gatgaacctt
    40741 gatttaaatc ccagggattc cgggtttttc ctccaaacca gacgtcaccc cgcgcaaggg
    40801 caccggaagt aagtttggcg atcaaaacgg ctcccgcgtg ttctaatctt tcaattaccg
    40861 ccgctgtgat ttcaatttgt tgctctttgt aaggaaagga tccccaggta gtgggatatc
    40921 cgggatatga gaacaaatcc ttgattccgt aaggaatgcc atgcaaatat ccccggtatt
    40981 ttccattggc aatttcttca tccgccctgg cagcttgttc aagcgcagtt ttctcagtta
    41041 aagttatcac agcttccagg gtatcgccat acgtcttcag gcgttccagg taaattcttg
    41101 ttaattcgga tgatgtcatt ttccctgact ggatgagagc cgctaattta tacaccggta
    41161 aaaaagcaat cccggcatct gaacctggca gctcaacttc ttccatttct aaatgcagag
    41221 aatcaggccc cgattcaggc attacaaaac tgtcaggccg tggatcaaat aataatggtg
    41281 gagcaatttc atgatttatc gtatatgtac gcattgtatc ataacctctc cggttcctca
    41341 aaagataaga atacatggta tcaatctccc atccttcaaa ctgaagtccg ataatttttt
    41401 gggctgattg aacatcttct ctcgtaaatg gctccttttc ctgggcacat gatagcatta
    41461 aaatgatggg ggaaaatatt aagatttgct tcatttccgg gtgaataatt ggttacttcc
    41521 atattaagat tattaattga tggatcaaaa ccattaatgt aattgaggtt aataagttta
    41581 tctttgaaat ttgacaatac aaatttcagc tggttatggt tcaaaggcat cttggtattt
    41641 ctgtttgttt aatactactg gtatacgctt ttcgttcaaa tggacaagtt gagcaaccac
    41701 acaatgcttt ggcagcatct tatcaggctg gctatgtatt tggaaccaat ggatttctcc
    41761 gcggagaaaa tcgcaaaaaa gaagatatta acttttacca aaacgcttct tttaaatttg
    41821 gtcgtcaaac ggacggtagc gagttgtggc accggagata caatttcccg ttttacggcg
    41881 caggttttca tgtcgcggac tacaattcgc cggatgaaat cgggactcca tttacagttt
    41941 ttggattttg ttcatttcca ttgcatagat ggcaaaacct gtctttgatt tatgatttgg
    42001 aggtgggggt ttcttttaat tgggaacctt atagcattga gcataatcca tacaatgtgg
    42061 taatggggtc caaaggtcaa ggctatgtcg gaatgggttt tttcgctgac tttaagctaa
    42121 gtaagcgctg gataatgaat gccggagtcc actttgcgca ttattcaaat ggcgcggtga
    42181 aacgacctaa ccttggcatc aactccatct ctccaaaagt aattttgaga tacaatcttt
    42241 atgaggatgt gattaattac gatcaccggg acgttccgga atttgattct aataatgaat
    42301 ggacagtctc actctatgcc ggcgaaaagc agattattac agaaactacc cttgattcta
    42361 ttctgaggtt taagagggaa aagcaccagg tttatggtct aaccaccagt tatcaccgta
    42421 agataagtta caagtctaag tttggaattg ggaccagttt cagttatgat ggttcaaata
    42481 actcccctga cagtgcatgg accgacaatc ttcagtggag tatttttcca tcttatgaac
    42541 ttgacgtggg taaatttacg ataattattc agccgggatt ttacctggtc agaacacaaa
    42601 cccttttcaa ttctgagcgg tggtatcaaa gagtggggtt taagtatcat atacttgaaa
    42661 atctttttgt cggtgtaaat gtacgggcat tttatttttc tgcggctgac tatcttgaat
    42721 ggaacctcgg gtaccggctt aattggcatt gattttggtt cagcattaat taatgattat
    42781 tgagcagtag agaatacgga ggttccatcc tttatcgtta cgggaactgc ctgcaatttc
    42841 aggttaaact gtactttgta ccttttggta agttgcccga caaaattttc tgtttctgaa
    42901 atctttacaa ggttcagggt acaacctcca aaaccaccac ctatcattct cgcgcccaaa
    42961 gcaccaaaac tccgggcttc atcaacaaga aaatctaatt cttcacagct tacatcataa
    43021 tagttgctca atcctctgtg ggtctcatac atcaacccac ccaattcatt gagattatca
    43081 gattccaagg ttcggatcgc cagctccacg cgttgcattt cagttaccac ataatagcac
    43141 cgtttgtata cttcttcagc catttcatgt ttcacttttt taagttgatc catatcgcaa
    43201 tctctcaggc tctccattat gccgaattta tttctcaaga ttttcactcc ttcatcacac
    43261 tgttcccttc tcttattgta agctgattca gccagagaat gcttcactcc cgtatcaagc
    43321 aataccaaac tgtaagaacc aagcgcaagt ttgtgatagg agtaatcaag agatcggcaa
    43381 tcaagccgga taatgtgatc ttctttacca aaaacattcg cgaattgatc catgattcca
    43441 catttcgtcc cgacgtaggt gtgttcagcg agttgaccca tttttgttat tgatagcaaa
    43501 tccagatcaa gattaaataa ctcttttaat gcaattagaa atccgcactc cattgctgcc
    43561 gagcttgaaa gtccggcacc taacggaatg tcacccccga aaaccgcgtt aaaattggtg
    43621 aattcgtatt tatttttgat taattgatca attacgccca agacataatt tggccaatct
    43681 ttcgcagatc gctcaatttt atcgagcgaa aaactgaacg tttcctccaa atcaattgca
    43741 taaatagcag attcagcatt atcggatacg gccaccccaa tccagattgc tttatcaata
    43801 gccgccggca aaacaatacc ctcattataa tcaacatgtt cacccagtaa atttattctg
    43861 cccggcgacc tgataattat cggttcctga tcataaatct ctctgaattt gttagaaatt
    43921 aatttgtgat tcatttatgt aatgtattaa ttaacatcaa cgtgtggcaa cacttccact
    43981 tttattttag gtgataattt gattgatttt caatgtcggc aaagtgtggc aaacgtaaaa
    44041 ggcctattaa tgaatgttac atcaaagtgt gccaacactt ccacttctta ttttaggtga
    44101 aaatttgatc aatcggcaaa gtgtggcaca cgtaaaaggc ctcagcaacc ggattaaata
    44161 acaggattca tagaactggc agcaacttct cccggctttc ttccgccaag ccatgcatca
    44221 agatcttttt gcctccagtc gtgatcaggt ttaagaatcc gggttccttc cgcatagtag
    44281 attattgtca ttacttctct tgtagaatct gtcttattcg gcggagcatt atgtaatgtc
    44341 catccggaat gaaaagtagc atcacctgct ttcaattcct gatatcgtga aacttccagg
    44401 ttattttccc ggatgtaatt gacaaagaag tcatcagagt catccgaaat ttcttgggcg
    44461 atcaaatatc cttgcttatg tgaaccggta acaaattgca taatacccat ttccaaagtt
    44521 aaatctacca ggggcatcca catggtaatc gttttgtcgg tatccaaagg ccagtagaat
    44581 tgatcctggt gccagggtgt gtgcccgccg ccagcctctt taaataacgc ctggtcatgg
    44641 taaaggcata ctcgctctac tcccattaat ttagccgcga cacttccaaa tctttttgcc
    44701 aaaacaaatt tcttaacggt ttcattttcc aaccaaagat ttttgatctg caaaaaagct
    44761 ttcccataag tatctctttc gctcaacggt cgtttttctt ctttgtttct ttctaccgct
    44821 tctcttatcg cattcctgta ataatatatc tccgcggagt tggcaagtcg gttaaccaaa
    44881 atataaccac agtcgttgta aaattttttt tgttcctccg ttaccgggta aggactgttt
    44941 aaaggaaaat ctttcaaggc cacaattcaa tttaaaaaga aaacgtaaat caattgttca
    45001 aatatatttt catagtaact aaaatgttta taattcattg caatttctga ttagttgtac
    45061 aaaaaacctg acaatttcat ggtagttatc aatagtaatt gactcattga taccatgaaa
    45121 tccggttaga ttttccggat ttattcgcat gggaagaaac cggtaaacat tattggttat
    45181 agagtaaaaa tgcgtagcat ccgttccacc gccaagcaaa cctggtgcaa caatcgtctc
    45241 gcccaaactc tgttttattg tttttgcaat cagttcaaaa gcaggtgatt ctatggttga
    45301 taccggagaa ggatttttgg ctgagccatc tatttcaatt gtgaagcgat catcattaat
    45361 aatattcact acatgctcat gaatcaattc aatattttcc cccggcaaaa tcctgaaatt
    45421 cataattgcc catgctgtgg tggggataac attcgctttc atacctccct gaattatggt
    45481 tggcgcggta gtagtgtgtg cattcattgc cttcgccacc gtgcctccga aaagccaggg
    45541 attagcaaaa accacttttt gcaaaaatgg taattcgggg cccatatatt tcatttgaga
    45601 atttatgggt tccaccaatt tatattcaaa ttgattttct tccaacctgt taatagcccg
    45661 ggttaacaat ccgatggtgt tatcaaccgg cggactagat gaatgacctc cggaagtgtt
    45721 cactgttatc ttgaatgaaa caaagccttt ttctgccaca ttgattaatg ccagggattt
    45781 gtcaattcct gacatcaact tatcaaccac ataacctcct tcatcaataa caagctccgc
    45841 tttaactcct ctcgattgca ataattttgc aatctcaccg gcgccttttg gtccacctac
    45901 ttcttcatca tgaccaaacg ccatatatat cgttcttgcc ggttcgaatc cctttgatat
    45961 taaaaactcg accgcttcca taatcgctat caaactcacc ttatcatcca ttgctcctct
    46021 tccccaaacc attccgtctt tgatttctcc cttaaaagga tcaacttccc attggtctaa
    46081 agtattttca tctaccggga ccacatcctg atgcgacatc agaataattg gattcagcgc
    46141 tttgttagtt ccatcccatt tatataacaa gctttcattt actttttcca ttggaataag
    46201 cgagtcaatt aacggataag ccgatttaag aaatttgtga aaggaatgga attctgcggt
    46261 atcaagtttt gaagtatctt cataggaaac agttttgaat tttattgctt ccgcaagatt
    46321 gttctccggg ctttccggaa tttcaatagt tacctgatca acagttatct gctttgactc
    46381 aaaagtagat gtgtttaaca tcaatattat taatactgcc aacgacagca gtaaaaagcc
    46441 atatcccaat tttttcatga cacaatattt ttttgaaaag tagaaaaaat cctcgttcca
    46501 atattcagat ttaatgaaat tta
//
LOCUS       DASWHX010000254        13746 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_2561376, whole genome shotgun sequence.
ACCESSION   DASWHX010000254 DASWHX010000000
VERSION     DASWHX010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 13746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13746
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2561376"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            66..719
                     /locus_tag="VGA21_15515"
     CDS             66..719
                     /locus_tag="VGA21_15515"
                     /inference="COORDINATES: protein motif:HMM:NF016214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEX9651969.1"
                     /translation="MFIGHFALGFASKKLAKPLSLGTSFLAVQFLDLLWPFFLIFGWE
                     TVEIDPGNTAYTPLNFISYPISHSLLTSLGWGILFGLVFYLVKREKGHAILLGFLVVS
                     HWVLDFMTHRPDLPLTPWGSMKVGMELWKSIPATIVVESLLFIAGVYIYLTSTKPRGL
                     KSWLVIMGLVFFLIIISIVNITSPPPPSAEMIGYAGFAQWLLVFWGYWIDKQRFEEN"
     gene            complement(1121..2683)
                     /locus_tag="VGA21_15520"
     CDS             complement(1121..2683)
                     /locus_tag="VGA21_15520"
                     /inference="COORDINATES: protein motif:HMM:NF013148.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium bicarbonate transporter family protein"
                     /protein_id="HEX9651970.1"
                     /translation="MKSTTKSSAHEGLEWTGRLFGGITDDIKRRAPHYIDDFKDGLHS
                     KVAGTTLFLYFAALANAIAFGALTGALTGNQIGITEMIVVTAVGGICFALFSGQPLTI
                     LGGTGPITIFTGLLYTACLQLDIPFLATYAWVGIWSGIILLICAFTDASALMQYFTRF
                     TDEIFAALISVIFIVEAAKDTINSFGPEGFGLSSGFLTFILAMGTFGISRYLKLTTST
                     PYLRKTLRDFLSDFGPSIAIVIMTAGAMLFPEVELNRPAVPDSISTTIGRPWLVNIFE
                     IPVWAIFASIGPAILATILLFLDQNITTRLVNSADNKLRKGMGYHLDLAVVGIIVLVG
                     SIFALPWIVAATVHSLNHVKSLATVKVVDVGGGQKKEKIVSVRENRVSGLSIHVMIAG
                     SLFLLNYFAYIPMPVLFGLFLYMGFASLGGNQFYERMMLWITDPKLYPDTHYTKNVPI
                     KVMHRFTLIQSLCFLALWILKTSVIGILFPLMIAALVPIRIFASRYFKKEHIAALVAE
                     DAEEEERIGVVM"
     gene            2979..4016
                     /locus_tag="VGA21_15525"
     CDS             2979..4016
                     /locus_tag="VGA21_15525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651971.1"
                     /translation="MEKNRKIFYAIGIVIIVAIAGYYFAGGNFGVSPEDAASSAMDGV
                     KKADKYTSGSSEEIAMDGEEVQQLLQNDQFQQMIRDEAFRELMKSPEFTALAKNPAFI
                     DLAKNPAFEAMAKNPQFLDMAKSPAFETLAKNPNFEVLAKNPEFMNLAKTAEFSALAK
                     SPQFEELMKSPEFMALAKNPAFADLAKSPSNFELMKNAAFEDLAKNPALADLAKSPSF
                     QALAKNPAFANLAMNPQFQALAKNPQFTALAKNPAFEALAKNPEFMALAKSPAFENLA
                     KSPQFADLMKSPQFGELMKSPQFLSLAKNPQFEAMAKNPAFTDLMKNQTFTSLMKNAQ
                     FADLEKLATMQ"
     gene            4137..7139
                     /locus_tag="VGA21_15530"
     CDS             4137..7139
                     /locus_tag="VGA21_15530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651972.1"
                     /translation="MNYRLTLLYLTFSFSAYAQLNLIETNEIRLVTYDFGHQYVLPHA
                     GRCFHNALEFHKKLFDYTPSEKITILIQDFGDYGNGGATAVPKNAVSIGLAPFSYAFE
                     TSPAGERIFSMANHELIHVLALDNATKTDRFYHKLFFGKVMPTKENPISMFYSFLTSP
                     RRYSPRWFHEGIASYIETWMSGGVGLAVGSYDEMVFRTRVLENARIYTALGLESEGTT
                     TDFQGRSNSYLYGTRFMGYLSYEYGPEKIIEWVKRNDGSKRTFSAQFKQVFGKPLATS
                     WDEWIEFEKEWQRVNAEQLRENPITDVKPITERNLGSVSYAFHNEKRNEVYVAVNYPG
                     QVPHLATINLNTGKLDKLVDIKGAALFYVTSLTFDPEAEILYYTTDNDNWRDLNSYNV
                     ETGKTKLLQKDVRTGDLAFNHVDKSIWGIKHLNGYSTITKIPRKNELEENTRPYSGWK
                     QIYTLPYGKDIFDIDISPDGRKMSAAVADTRGNQLLLIFDLMKLEQGEVSADTVFNFD
                     ITSPQSFRYTKDGKYLYGTSYWSGVSNIYRVETESLEIAPVTNAVTGFFRPLLINDEQ
                     LFAFEFRSDGFQPVYIKNELVENINTVNYLGTKTIAKHPMLKDWETHPPRPSEMNFDE
                     MKIEEGQYRAGKLLKVNYAYPTVVGYKDFVGLGYLLNIGDPFGFRELEFEFAYTPRSF
                     RNGLTESDSLNSILSDEELYHFSAYAKLGRFEMYGSFNGTDFYDLFGPTKTTRKGIQF
                     GVDYLTTLIYDPPRNLDLNLGISTFYGLEKSPEFQTINTTASDNNFFVNLRGGLTYRY
                     MTNSLGAVDPEKGIRVSLRPAAAYSAGSIYPRAILSLDYGIQMPVNHFSIWIRTAAGK
                     AFSNTLNPFTRFGFAAFGNNYIDHQTSRRYRTEFSFPGVQYDADVTIIAKEFAKGMIE
                     FVLPPIRYRELGFLNFYANWTQLSIFTSGLRADNNQQFVNFGAQLDTRLVLFSLMPST
                     LSLGYASAFDLVTENNYNEWMISLKILR"
     gene            7214..7933
                     /locus_tag="VGA21_15535"
     CDS             7214..7933
                     /locus_tag="VGA21_15535"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HEX9651973.1"
                     /translation="MNSVFLVLMALAPGIAVTLYIYLKDEYEPEPIWLLLLSFLYGCI
                     AFGITYVFDFAIYKIIHLEAQNLFHQGIRALILVGLVEECSKFIFVRGLLYPNKNFDE
                     PFDGIVYAVMVGMGFATIENIIYVINGGGGTAIVRMFSAIPAHAVFAILMGFFLGKAK
                     FDKKNERLNVWLGLIVATVYHGVYDYFLFISFVAGIWILAGVSLVIAILLSRISIRMH
                     HDASPFKNNPKEQHRYPKEPQ"
     gene            complement(8163..9236)
                     /locus_tag="VGA21_15540"
     CDS             complement(8163..9236)
                     /locus_tag="VGA21_15540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017732296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ice-binding family protein"
                     /protein_id="HEX9651974.1"
                     /translation="MNNIKIASVISLLLIFSVVGCKNDDAEIIQIESPTVTLTNPLNN
                     STGVTRNTAVVVTFSEAMNASTINTETFTLTQGTIVVPGTITYVENTATFLPLVALEA
                     GEQYTANVSTGVKDLDGNPLATKVTWNFTTGASSSTQQAINLGTAGSFLVLAKTAINN
                     NPTSIMTGDLGLSPAATSYITGFALTDETGYATSAQVTGKVYAADMADPTPINMTTAV
                     ENMITAYNDAAGRPTPDYFELNTGNIGGLTMTPGLYKWTNTVLIASDVTISGSATDIW
                     IFQIAGDLTMSSAVNVTLIGGALPENIFWQVAGTVTLGTTSHFEGIILSMTGITLNTG
                     ASMNGRALAQTAVVLDGNTVIKP"
     gene            9257..9433
                     /locus_tag="VGA21_15545"
     CDS             9257..9433
                     /locus_tag="VGA21_15545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651975.1"
                     /translation="MVKEYQLAMNGEYSGRVNDFKKTNLAWLPGVQVGFRKIDFLKLL
                     TTNLQVLSMVSVTQ"
     gene            9684..9839
                     /locus_tag="VGA21_15550"
     CDS             9684..9839
                     /locus_tag="VGA21_15550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019944387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lmo0937 family membrane protein"
                     /protein_id="HEX9651976.1"
                     /translation="MGNLLYAIAIILIILWAIGYLGFNSGGIIHVLFIIAVIAVLFRV
                     IRGSRIV"
     gene            9954..10595
                     /locus_tag="VGA21_15555"
     CDS             9954..10595
                     /locus_tag="VGA21_15555"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HEX9651977.1"
                     /translation="MKKILLLILAISSIAYPGKAQETEIDLRQRFIFGVKIGANLSTV
                     FDTQGEQFNADPKIGFVTGAFLTVPIWRFLGVQPEILFSQRGFKATGVILGSPYKFTR
                     TISYIDLPLLATFNLNNVVTVVAGPQLSFQVWQRDAFSTGTSPTQVQEFSNDDIRKNT
                     LSILTGLDFNFNHIVLGTRAGFDVQDNIGKGTVITPRYKNVWVQATLGYRFYN"
     gene            10882..11106
                     /locus_tag="VGA21_15560"
     CDS             10882..11106
                     /locus_tag="VGA21_15560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018341781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651978.1"
                     /translation="MRTRNSAFILIVLGILMLIYTGFNFVTTERVVDLGPLKIDKQKN
                     HSVAWPPIVGIVLVVGGVFLLGKTKKGRAI"
     gene            11623..13167
                     /locus_tag="VGA21_15565"
     CDS             11623..13167
                     /locus_tag="VGA21_15565"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX9651979.1"
                     /translation="MKKFYLLLLLPVIVNAQNASVEASRAWKKKEGHNIIANFTELLS
                     IPNRASDLPNIRKNAELISKMFSDRGFQMQLLEIEGSPPAVYGEKKVLNAQRTICFYV
                     HYDGQPVDPSSWSNAPFQPVLYDKAMYAGGQPIAMPKANQAIEENWRIYARSASDDKA
                     PIIALIAAIDALKAVKIDYTSNIKFFFDGEEEAGSPHVTQFFNKYSSLFKDINFWLLV
                     DGPVFQTGAPTLKFGGRGFTGLEITVYGATRPLHSGHYGNYAPVPGQMLASLLASMKD
                     EKGKVLIAGYYDSVDPVSDFEREQIKKIPNIEKELKADLGLMFVEGDNETLFERLLLP
                     SLTVKGLQSGNVGSLARNVIPNTAIANLGLRLVKGNDPEKMLDLVEAHIKKQGFHIVY
                     EEPDLEIRRTYQKIVKVARSKSGSPAAKVSMDHPELLPVIQAVKSFTGDDLVLLPSEG
                     GSNGVFTAVFDGLGQPGISVNIANHDNNQHAEDENIRIGNLWYGIDLMSVLLTMPGGN
                     ETFKKK"
     gene            13190..>13746
                     /locus_tag="VGA21_15570"
     CDS             13190..>13746
                     /locus_tag="VGA21_15570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M20"
                     /protein_id="HEX9651980.1"
                     /translation="MKKLLLLLIIPCIGYSQNKVVDATRNWKEKEGYNILASFAELLS
                     IPNHGSDAVNIRRNAKLIGENFTNRGFKMQLLEVEGAPPAIYGELKTPNAHRTVCFYV
                     HYDGQPVDPSRWVNPPFEPTLYSNALFNNGKPIPMPKAGDKINENWRLYGRSTSDDKA
                     PIIALLAAIDALQNSEIAFTSNIKL"
BASE COUNT         4145 a         2699 c         2870 g         4032 t
ORIGIN      
        1 gcccatgtaa ttgagtttac cgattaatat tttcaatttg gtcaggtaaa taaacctggc
       61 ggaaaatgtt tattggtcac ttcgctttag gtttcgcctc taaaaaactt gcgaaacctc
      121 tttctctggg aacatcattt ctcgcagttc agtttctgga tctcctgtgg cccttttttc
      181 tgatatttgg ttgggaaaca gtggagattg atcccggtaa tactgcttat acccccttga
      241 attttattag ctacccaata tcccatagtc tgcttacttc tctgggttgg ggaatactct
      301 tcggtcttgt cttttattta gttaagagag aaaaaggtca cgccatatta ctgggttttc
      361 ttgtagtcag ccattgggtt ctggatttta tgacgcaccg ccccgattta cccttaactc
      421 cctggggaag tatgaaagtg ggaatggaac tgtggaaatc aattccggca acaattgttg
      481 tcgagagttt attgtttata gcaggggttt atatttatct aacttccacc aaaccccggg
      541 ggttgaaatc atggctggta ataatgggac tggtgttttt tctgatcatc attagtattg
      601 tcaacataac ttctcctccg ccaccatcag cagagatgat tggatatgcc ggatttgctc
      661 aatggctgct agtgttctgg gggtattgga ttgataaaca aagatttgaa gaaaattaac
      721 ccctgaatga ttttctctgc ggtgatctgc gaaatcagcg gtatcaagta aaatcttccc
      781 cacagattac gcgggatttg cgcagaaatc tttgaataat tttctctgca tgatctgcga
      841 ttatcagcgg gattaagtaa aattttctcc cacagattac gcggatttgc gcagaaatct
      901 ttgaattatt tttttcttcg gtgatctgcg atatcagtag gattaagtct tctcctgcag
      961 aaatcctgaa tgattttctc tgcggtgaac tgcgattatc agcggtatca agtaaaattt
     1021 tctcccgcag attacgcgga tttgcgcaga aatctttgaa tgattttttt ctgcggtgat
     1081 ctgcgatatc agcgggatca atgatttaca gaaactctgt ttacataacc acgccaattc
     1141 tttcttcttc ttcagcatct tccgccacaa gagcggcgat atgttccttc ttgaaatacc
     1201 tgctggcaaa aattcgtatg ggtactaaag cagcaatcat caacggaaaa agaattccta
     1261 ttacagatgt ttttaaaatc cacaacgcca aaaagcacaa ggattgaatc aaagtgaacc
     1321 ggtgcataac ctttataggg acgttcttgg tataatgcgt atcgggatag agttttggat
     1381 ctgtaatcca aagcatcatt ctttcgtaga attggttacc ccccagtgaa gcaaatccca
     1441 tataaaggaa taaaccaaat agaaccggca ttggaatata agcaaagtaa tttagcagga
     1501 acaaggagcc tgcaatcata acatggatag ataatccgga aactctgttt tcgcgaaccg
     1561 aaactatttt ttctttcttc tggcctccgc caacgtcaac aactttcacc gttgccaaac
     1621 ttttgacatg atttagtgaa tgcacagtgg ccgcgacaat ccagggaagt gcaaaaatgc
     1681 tacccaccaa aacaattatg ccgacaaccg ccagatccag gtgataaccc attcccttcc
     1741 tcaatttatt atctgctgaa tttacaagcc gggtagtaat attctggtcg agaaacagaa
     1801 gtatggttgc aagaatcgct ggaccaatag acgcgaagat tgcccataca ggaatttcaa
     1861 agatgtttac cagccatggt ctgccaatgg tggtactaat actatcagga actgcaggtc
     1921 tgtttaattc aacttcagga aataacatgg ctccagctgt cattataacg atggcaatgg
     1981 aaggtccaaa atcacttaga aaatctcgga gcgtttttct gaggtaggga gtggaggttg
     2041 taagctttag gtacctggaa attccgaaag ttcccatggc cagaataaag gtcaggaagc
     2101 cgctcgataa tccgaaacct tcaggcccga atgagttaat ggtatctttg gccgcctcaa
     2161 caatgaaaat tactgaaata agagccgcaa agatttcatc cgtgaaccgc gtaaaatact
     2221 gcatcaatgc gctggcatct gtgaaagcgc aaatcaaaag tataatgccc gaccaaattc
     2281 ctacccaggc gtaggtagcg agaaagggta tatctaactg gagacatgct gtatataata
     2341 gtcctgtgaa aatggtaatg ggtccggttc caccaaggat tgttaagggc tggcctgaaa
     2401 ataaggcaaa gcaaatgcca ccaacggcgg ttacaacaat catttcggtt ataccaattt
     2461 gatttccggt taaagccccc gttaaagctc caaaagcaat tgcattggct agagccgcaa
     2521 aatataaaaa caaggtagtg cccgcaacct tcgaatgcag cccgtctttg aaatcatcaa
     2581 tataatgggg agcccggcgc tttatatcat cggttattcc accaaataat ctccctgtcc
     2641 attctagtcc ttcatgtgct gatgattttg tagtactctt cataaatttt cttattgaaa
     2701 tatttttttg gtttagatag ttttagaaag gatattgcta agaaagaatt tcaatatttt
     2761 gctccaatgg cgcacataca taaataaaag ttacgttgac taaatcattc atataagccc
     2821 gtaatccaac tttccttgac ataacttgca cgtaaaatgt tgaaaatcag tggtgtatat
     2881 aaaggggtta ccttattatc ctaattggaa attaaggaat aaatatgcta actttatttt
     2941 cccctaacaa ctaggattca aactaaactt acttaaaaat ggaaaaaaat cgaaaaattt
     3001 tttatgcgat cggtatagta ataatagtag cgattgcagg gtactatttt gctggtggaa
     3061 attttggagt ttctccggaa gatgcagcaa gttctgccat ggatggtgta aagaaagctg
     3121 ataagtacac tagtggttcc tctgaagaaa ttgcaatgga tggtgaagaa gtacagcaac
     3181 tgcttcaaaa tgaccaattc cagcaaatga tcagagatga agcgtttagg gaactcatga
     3241 aatctcctga atttacggca ctagctaaga acccggcatt tattgatctc gctaagaacc
     3301 cagcatttga agcgatggct aagaacccac agtttttgga tatggcgaag tcgccggctt
     3361 ttgagactct tgccaagaat ccgaattttg aggtcttggc taaaaatccg gaatttatga
     3421 atcttgccaa aacagcagaa ttcagcgcgt tggcaaaatc acctcagttt gaagaactca
     3481 tgaaatctcc ggagttcatg gcgctggcta agaatcctgc ctttgcagat ttagccaaga
     3541 gtccttcaaa ttttgaactg atgaaaaatg cagcttttga ggacctggcg aagaaccccg
     3601 ctttggcgga tctggccaag agcccgagct ttcaagcttt ggcaaagaat cctgcttttg
     3661 cgaatttagc catgaatcca cagttccagg ctcttgcgaa gaaccctcaa ttcactgctt
     3721 tggctaagaa tccggctttt gaggcactgg ctaaaaatcc cgaattcatg gctctggcaa
     3781 aatctccggc gtttgaaaat cttgcaaaat cgcctcagtt tgctgacctg atgaaatcgc
     3841 ctcagtttgg agagttgatg aaatcacctc aattcctgag tctggccaaa aatcctcaat
     3901 tcgaagcgat ggcaaagaat cccgctttta ctgatttgat gaagaatcaa acttttactt
     3961 cacttatgaa gaatgcacaa tttgctgacc tggaaaagtt ggctaccatg caatagtaag
     4021 aattcataaa acaacccaaa cttaccatgg ggagacagct tgactgcctc cctttctttt
     4081 tgtcattcaa attatttcac taatttgttt gccaagctcc agcctaaacc ctgattatga
     4141 attacagact caccctcctg tacctgacat tctccttttc tgcttacgca cagcttaact
     4201 taattgaaac aaacgaaatc cggcttgtga cctatgattt tggtcatcaa tatgtacttc
     4261 ctcatgccgg aaggtgtttc cacaatgccc tggaatttca caaaaagctt tttgattata
     4321 ccccttcaga aaagatcacg atattaattc aagactttgg tgattatggc aacggtggag
     4381 ccactgctgt tcctaagaat gcagttagca taggtcttgc tccatttagt tacgcttttg
     4441 aaacaagccc ggcaggagaa agaattttta gcatggctaa tcatgagctt attcacgtac
     4501 tggctcttga caacgccaca aaaacggatc gcttttatca caagctattt tttggaaagg
     4561 ttatgcctac taaggaaaat ccaatttcaa tgttttatag tttcttaacc agtccccgga
     4621 ggtattcgcc ccgctggttt cacgaaggta ttgcttccta catagaaacc tggatgagcg
     4681 gaggagtagg acttgcagtg gggagttatg atgaaatggt ttttagaacg cgggtgctgg
     4741 aaaatgcaag gatttatact gcccttggtc tggaatcgga agggacaacc acagatttcc
     4801 agggaagatc caactcatat ttatacggca cacggtttat gggatatctg tcctatgaat
     4861 acggaccgga aaaaattatc gagtgggtaa aacgaaacga tgggagcaag cgaacttttt
     4921 ccgcgcaatt caaacaggtt tttggcaaac ccttagctac cagttgggat gaatggatcg
     4981 aatttgaaaa ggaatggcaa agagtaaatg ctgagcaatt aagagaaaat ccgataacag
     5041 atgtgaaacc cattacagaa agaaatctcg gctctgtttc ttatgctttt cataatgaaa
     5101 aaagaaatga agtatatgtc gcggtaaatt acccgggaca ggttccgcat ttggcaacca
     5161 taaacctgaa taccgggaaa cttgataagc ttgtagatat taaaggtgcc gctttgttct
     5221 atgtaacctc gctaactttt gaccccgaag ctgaaatact gtactatact acagataatg
     5281 ataactggag agatttaaac agttataacg ttgaaacagg gaaaaccaaa ctcctccaaa
     5341 aagatgttcg aaccggagac cttgcattta accatgtaga taagagtatt tggggtatta
     5401 aacatcttaa cggatattct accataacga aaattccccg aaagaatgag ttggaagaaa
     5461 ataccaggcc gtacagcgga tggaagcaaa tctatacact gccttatgga aaagatattt
     5521 ttgatataga tatttcacct gatggcagaa agatgtcggc agccgtagcc gacacacgcg
     5581 ggaaccaact tttattaatt tttgatttaa tgaagctgga gcagggtgaa gtttctgccg
     5641 atactgtatt taattttgat attacctccc cgcaaagttt caggtataca aaagatggaa
     5701 agtatttata cggtacttca tactggagcg gtgtgagcaa tatatacaga gtggaaaccg
     5761 aatctttgga aatcgcaccc gtaaccaatg ctgtaaccgg attttttagg ccactgttaa
     5821 tcaatgacga acaattgttt gcatttgaat ttcgcagcga tggattccag ccggtttaca
     5881 ttaagaatga gttggttgaa aatattaaca ctgtaaatta tctgggtaca aaaacaatcg
     5941 ctaagcatcc aatgttaaag gattgggaaa ctcaccctcc ccggccatcg gaaatgaatt
     6001 ttgatgagat gaaaattgaa gaaggccagt acagggccgg taaattactc aaggttaatt
     6061 atgcctaccc aacagttgtc gggtataagg atttcgtggg tttaggatac ttgctgaata
     6121 ttggtgatcc ttttggattt cgggagttgg agtttgaatt tgcttataca ccacgaagtt
     6181 ttagaaatgg attaactgaa agtgattccc tgaatagcat cctgagtgat gaagaattat
     6241 atcatttctc cgcttatgcc aaacttggaa ggtttgagat gtatggctcg tttaatggaa
     6301 ctgattttta tgatttattt ggtcctacaa aaactacacg gaaagggatc cagtttggtg
     6361 tagattacct taccacactt atttatgatc caccacgaaa tctggacctt aacctgggta
     6421 ttagtacgtt ttacggtctg gaaaaatcac cggaattcca aacaattaat actaccgctt
     6481 ccgataataa cttcttcgtg aatttacgag gaggactaac ctaccggtat atgacaaatt
     6541 ccttaggtgc cgtggatccg gaaaaaggaa ttcgcgtgtc tctcaggcct gccgcggcat
     6601 attctgcagg aagcatttat ccgcgggcaa ttttaagcct tgactatggc atccagatgc
     6661 cggttaacca tttttctatt tggattagaa ctgctgctgg aaaggcgttc agtaacacat
     6721 taaacccttt caccaggttt ggatttgccg cgtttgggaa taattatatt gatcatcaga
     6781 ccagcagaag atacaggaca gagttttcat ttccgggtgt gcaatatgat gctgacgtaa
     6841 caatcattgc caaagaattt gccaaaggca tgattgaatt tgtcttgcct ccaatcagat
     6901 atagagaatt aggtttcctg aatttctatg ctaactggac tcagctcagc atatttacat
     6961 cgggccttcg ggctgataat aatcagcaat ttgtgaattt cggtgcacag cttgacaccc
     7021 gtttagttct gttttcgcta atgccatcta cactatccct cggatatgca tcagcctttg
     7081 atctggtcac tgaaaataac tataacgagt ggatgatttc actaaagatc ttgcggtgat
     7141 aattctaaaa attcgaaccg gttaatccgt ttggataact ttagaattat attacctttt
     7201 taaattgaaa taaatgaatt cggttttcct ggttttgatg gcactggctc cgggtatcgc
     7261 ggttacctta tatatctatc tcaaggatga atatgaaccg gagcctatat ggctgctgct
     7321 gttaagcttc ttatatggat gtatagcatt tgggatcacc tatgtatttg attttgcaat
     7381 ctataaaatc attcaccttg aagctcaaaa tctttttcac cagggaatca gggccttaat
     7441 cctggttggg ctggtggaag aatgttctaa gtttattttt gtcagagggt tgctataccc
     7501 aaataaaaat ttcgatgaac cctttgatgg aattgtctat gccgttatgg ttggaatggg
     7561 atttgccacc attgaaaaca taatttacgt aatcaatggt ggtggcggta ctgccattgt
     7621 acggatgttt tccgcaatcc ctgcgcatgc ggtctttgct atactgatgg gtttttttct
     7681 gggcaaagcc aaatttgata agaagaatga acgcctgaac gtatggctgg gcttgatcgt
     7741 ggctactgtt tatcatgggg tgtatgatta cttcttgttt atttcatttg tagcaggtat
     7801 ctggatcctc gcaggagtat ccctggtaat tgctatcctc ctttccagga tttcaatcag
     7861 gatgcaccat gatgcttcac cattcaaaaa caaccctaaa gaacaacacc gttatccaaa
     7921 agaacctcaa taatgaattg gcaataccgg gattccagag ttcaagagac aaaaatcgcg
     7981 tcctacgttt ttgataactc attgcggcgg aaaacggaat gacaatctcc agaattattt
     8041 agtatgtcat tcctgtttct gccaggtgtt tcaaaagcac aaaaccggga tccagttact
     8101 tttcattcaa aaaaaagaag caacttatag ttgcttcttt ttttagattt ttgttgagag
     8161 gcttacggct taattaccgt attgccatca aggacaactg ctgtctgtgc cagagcacgg
     8221 ccattcatac tggctcctgt atttaaagtg atacctgtca ttgacaaaat aattccttca
     8281 aaatgagagg ttgttcccag ggtaaccgta cctgcaacct gccagaaaat attctcaggc
     8341 aaagcgccgc ctattaaagt aacgtttacc gctgaactca tagtgaggtc tccggcaatt
     8401 tggaatatcc aaatatcagt agcactacca gaaatggtaa catccgatgc tataagtacg
     8461 gtgttggtcc acttgtataa tccgggagtc attgtaaggc caccaatgtt tcctgtgttt
     8521 agctcaaaat aatcaggagt tggtcggcct gctgcatcat tataagcagt tatcatattt
     8581 tcaactgcag ttgtcatatt tataggtgta ggatctgcca tatcagcggc ataaactttg
     8641 cctgtcacct gcgctgaggt tgcatatccg gtttcatctg ttagggcaaa gccggtgata
     8701 taacttgttg cagccggact tagtccaagg tctcccgtca tgatcgaagt gggattattg
     8761 ttaatcgcag tctttgcaag gaccaaaaag cttcctgcag taccaagatt tattgcctgc
     8821 tgagtagaag acgatgctcc ggttgtgaaa ttccatgtca ctttagttgc aagtggatta
     8881 ccatccagat cttttacacc tgtactaacg tttgcagtgt actgctcacc ggcttccaga
     8941 gctactaagg gtaaaaatgt tgctgtattt tccacataag taatggtacc gggaacaaca
     9001 attgttccct gagtcagggt gaatgtttcc gtgttaatgg ttgaagcatt catcgcttca
     9061 ctaaatgtaa ccactactgc tgtgttacgc gtaaccccgg ttgaattatt caagggatta
     9121 gttaaagtca ccgtagggct ttctatttgg attatttcag cgtcatcatt cttgcaaccg
     9181 acgacggaaa aaatcaaaag taaactaatg actgatgcaa tttttatatt attcattgtt
     9241 ataaatttaa tcgcatttgg ttaaggaata tcaacttgcg atgaatggcg aatattcggg
     9301 aagagttaat gatttcaaga aaactaatct tgcctggttg ccaggagttc aggttggatt
     9361 tagaaaaatt gattttctaa aacttcttac aacaaatttg caggtattaa gcatggtaag
     9421 cgttacacaa taatcatttt atgttacata tatcacagct taattgttta attaactcca
     9481 gttagtggtc ctgatcatcc caaagggcat aaacttctat atctgtgata tatgtaatac
     9541 ttatgaattc ttatataaca gtttataaag agaattgata caatatttgt atgtatttga
     9601 aatagaacaa aataaaacaa gaagggaaaa gagtgattga ggcgtagttt taccaaatca
     9661 taatgaaatt aaaaaacaat aaaatgggaa atttacttta tgctatcgcg attattctga
     9721 ttatcctctg ggcaatcggg tatttaggat ttaactccgg cgggataata catgtcttgt
     9781 ttattatcgc cgttatagcc gttctattcc gggtaatcag aggtagtcga attgtttaat
     9841 actgctcata aaattttttg agccggggtg gatttgaata tctcaatatg caaagttgtt
     9901 ctatcatcag ggttaatttg aatctgaaat aaatttaaaa taataataga ctcatgaaaa
     9961 agatactact attaatattg gcaattagct caatagctta cccgggtaaa gcccaggaaa
    10021 cggagattga tttacggcaa aggtttattt ttggtgttaa aattggagcc aatttatcaa
    10081 cagtgtttga tacacaaggt gagcaattta atgcggatcc caaaattggt tttgtaacgg
    10141 gtgcattttt gacagtacct atttggagat tccttggcgt tcaaccggaa atactatttt
    10201 ctcagcgagg attcaaggca acaggtgtta ttcttggcag cccttataaa tttactcgta
    10261 caataagtta tattgatttg cctttgctgg ccacgtttaa cctaaacaat gtagttacgg
    10321 tggttgcagg accacaactc tcttttcagg tttggcaacg agatgcattt tcaacaggaa
    10381 cttctcccac gcaggttcag gaatttagta atgacgatat tagaaaaaat accctgtcta
    10441 tattaacagg attggatttc aattttaatc atatcgtact gggaacaagg gcagggtttg
    10501 atgttcaaga taatatcggg aaaggcacgg tgattactcc gcgctataag aatgtctggg
    10561 tgcaagctac tctgggttac aggttttata attagataat taggaataaa aagttcatca
    10621 tgaaaaatgg atcgagaaaa atgagattgt ggaatctgat aatactaacc ttatgcctaa
    10681 ctcttttgtt tagcgtaaca tcctgccttg ttttggttcg caaagataat ggaaagcatc
    10741 ggggttggtt taaaaattca aaaagccatc cgcggcatac caaaccggga aaagcgaagg
    10801 gtaaccatga ggagagtact tcaaaacttt ggtgggagat agataaaaac ttaatatcac
    10861 ataatataaa aaaatagaat tatgagaact agaaactcag ctttcatcct tattgtactg
    10921 ggaatattga tgctaatcta taccggattt aattttgtta ctaccgaaag agtagtggat
    10981 ttaggcccat taaaaattga taaacagaag aatcactctg ttgcatggcc gcctattgtt
    11041 ggaatagtgc tggtagtagg cggagtcttt ttgcttggta agactaaaaa agggagagct
    11101 atttaatgct tcttttattt tgattttaat ggggttagca ggtattgtcc gctgttgctg
    11161 ttgcttcatt gacttcactt ggaacttcaa atttagtatg tcaaaaaatt gcagactaag
    11221 ttttttgata actcagatgt tatctagtat gtcctctcgg tttctgccag agtgtttcat
    11281 aagcgcaaaa ccggcaatac cgggatccag ggatgcaagc tacgaaatcg ccaggctacg
    11341 tttttgcaac tcatagtggg caaacctaaa gggaatgaca atcaaatttt gccgccaagc
    11401 ctggacaagt agtaattacc ctggtttccg gttttgctgt cgaacagcaa ttaataaacg
    11461 agagcagaaa cctgaatgtc aatctccaaa ctattcgtag tttgtaaccg cgaagcaaaa
    11521 actcacccca cttatcaaaa aacccccgct cttaatccgg tttatgaaac ttagaattaa
    11581 cttacacatc cagttcagct tctaatatta aaacaatttc ccatgaaaaa attctatctc
    11641 ctgttacttc tacctgttat agtaaatgcc caaaatgctt cagttgaagc aagcagagcc
    11701 tggaagaaaa aagaaggtca caacataatc gcaaatttta cagaactcct ttcaattcca
    11761 aaccgggcgt ctgatttgcc caatatccgt aagaatgcgg aacttatcag caaaatgttt
    11821 tccgaccgcg gatttcaaat gcaactgctc gaaatagagg gatctccgcc cgctgtctat
    11881 ggggaaaaga aagttcttaa tgcccagcgt actatctgtt tctacgtcca ttacgacggc
    11941 caacccgttg acccgtcctc atggagcaac gctccttttc agcctgtgct gtatgacaaa
    12001 gctatgtatg caggagggca gccaattgcg atgcctaaag ccaatcaagc catcgaagag
    12061 aactggcgaa tctatgcacg gtcagcaagt gatgataaag ctcctattat agcgttgata
    12121 gccgccatag atgcattgaa agcagtaaag attgactata cctcaaatat taaattcttt
    12181 tttgatggag aagaagaagc aggttctccg cacgtaactc aattctttaa taaatattct
    12241 tccttattta aagacatcaa cttttggctt ttggttgatg gcccggtatt tcaaacagga
    12301 gctccgactc tcaagttcgg aggcagaggt ttcaccggat tggagattac tgtttatggt
    12361 gcaacacgac cacttcacag cggacactac ggaaactacg cccctgtacc cggacaaatg
    12421 cttgcaagtt tactggcttc catgaaagat gaaaagggta aggtattaat agcgggatac
    12481 tatgattctg tagaccctgt ttcagatttt gagcgggaac aaatcaaaaa aatccccaat
    12541 attgaaaagg aattaaaagc agatctgggc ttgatgtttg ttgaaggtga taacgaaact
    12601 ctttttgaac gcctgctttt gccatcgctt actgtaaaag ggttacaaag tggcaatgta
    12661 ggttctcttg ccagaaatgt aattccaaat acagccatag cgaaccttgg acttcgatta
    12721 gtcaaaggaa atgacccgga aaaaatgctt gacctggtgg aagctcatat taaaaagcag
    12781 ggatttcata ttgtttatga ggaaccggat ctggaaatca ggaggacgta tcaaaaaatt
    12841 gtcaaagtag ccagaagtaa gtccgggtca ccggcggcaa aagtttctat ggatcacccg
    12901 gagttgctgc cggtcattca ggctgtaaaa agctttactg gagatgattt ggttctgctc
    12961 cccagtgaag gtggctccaa tggagtgttc acagccgtct ttgatggcct tggtcaacct
    13021 ggaatttcag tcaacattgc taatcacgac aacaatcaac atgcagaaga tgaaaacatc
    13081 cggattggta acttgtggta tggcatcgat ttgatgtcgg ttttactcac tatgccaggc
    13141 ggtaacgaaa ccttcaaaaa gaaataactt ccaaattaaa ataaagtcca tgaagaaatt
    13201 gctcttgctc ctgataatac cttgcatagg ttactcacaa aataaagttg ttgatgcaac
    13261 ccggaattgg aaagaaaagg aaggttataa tatcctggca agttttgctg aattactttc
    13321 gattccaaat catggttccg atgcggtcaa catccgccgg aatgcgaaac tgattggaga
    13381 gaactttaca aaccgtggtt ttaagatgca attgctggaa gtggagggcg cccctccggc
    13441 aatttatgga gagcttaaaa ccccaaatgc ccaccgaaca gtttgctttt atgtgcatta
    13501 cgatggccag cctgtagatc cctcccgatg ggtaaatcca ccctttgagc caacactcta
    13561 cagtaatgcg ctcttcaaca acggcaaacc tataccgatg ccaaaagccg gagacaagat
    13621 aaacgagaac tggagactat acggccggtc gaccagtgat gataaagctc ccataattgc
    13681 attattggca gcaatagatg cgctccaaaa ttctgaaata gctttcactt caaatatcaa
    13741 actttt
//
LOCUS       DASWHX010000255        11422 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_334055, whole genome shotgun sequence.
ACCESSION   DASWHX010000255 DASWHX010000000
VERSION     DASWHX010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 11422)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11422)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11422
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_334055"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1459
                     /locus_tag="VGA21_15575"
     CDS             <1..1459
                     /locus_tag="VGA21_15575"
                     /inference="COORDINATES: protein motif:HMM:NF037374.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBG domain-containing protein"
                     /protein_id="HEX9651981.1"
                     /translation="TVTADPEQTKNYGSSDPVLTYQVTPALIGGDTFTGVLERAAGED
                     VDTYEINNGTLDGGGNYTITFEISNFTITPKTLSVTGITISNKEYDGTTDAIISGTPT
                     LEGIVGSDVVSLGSGSLSANFEDANAGSGKNVTVSGITLTGTNAGNYTLSTTLPVTAN
                     ITPRAIAVTANTNQSKTYGSSDPILTYQVTPSLISGDTFTGALSRGSGENTGTYSITM
                     SSLSAGGNYTITFVGEDFTITPLAITVTANPNQNKVFGSTDPALTYSVNPSLVAGDSF
                     TGQLSRVVGENAGSYAISIGTLSAGSNYQTVFESGNFIIEKASHTITFDLIEDRFNTS
                     EPFTVNATAGSGLEVMFNILSGPATVNGSSVTLTGETGTATIRASSEDENHLPATADQ
                     SFEITLDPVLGMEETWKRDIVIFPVPSDGMITIRSGEQEMQKVFLLDISGKLIWKSSQ
                     EKPVREMEIEIQEKGEFLVFIQTGKGMISRKIIKY"
     gene            1528..2769
                     /locus_tag="VGA21_15580"
     CDS             1528..2769
                     /locus_tag="VGA21_15580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651982.1"
                     /translation="MRKCISVIIVFLIIGGCEDDYPIKKGNIQFSFSSLSGSGGRMET
                     LTKPVAVLLSIEDNDGNSIEQNKKILLYTFGNGYVSESLQLATGTYQLAQFMVLDADN
                     KVIYATPVEGSELAQYVNDPLPIEFVVSDDGTTLVTPQVLAVDADNTPENFGYVSFSF
                     EVVSPLPRLTQIKVFEGGLSYATHMLDYTYSNNVLDEVKFYYCYPDEQNCNTSAWYKF
                     EYYPDGRIKGRYLYDAITGNVVETDIYEYEGILTKKINHYYDQYNTSNAVEFFYDQNN
                     KLTHSTLSSGYSSENSNYRLSEYTHNEDGSITEHVYLDGLNKDGLRFIFTYTFDQYNT
                     PSLYNTGIAEWVMFPVNKNVASITLKEIYQAGDPPPDCSTNQAYQYNALGLPESYVTS
                     NPENCYLGFMDGTVVQYFYNQ"
     gene            2876..3382
                     /locus_tag="VGA21_15585"
     CDS             2876..3382
                     /locus_tag="VGA21_15585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014798959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX9651983.1"
                     /translation="MIETERLILKPLTYNQLIKYIKADNSLEVELDLNESSRTISPEL
                     KEALEQTILPNVADITKDYLYSTLWTVISKEANKMIGDICIVGEPNSNGEIEIGYGTY
                     DEFQKKGFMTEAVGGMIKWAKSQTKVFSIIASTEKENIASFTILLKNNFIKVGETEAL
                     FIWKLTLK"
     gene            complement(3647..4447)
                     /gene="ispE"
                     /locus_tag="VGA21_15590"
     CDS             complement(3647..4447)
                     /gene="ispE"
                     /locus_tag="VGA21_15590"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019599308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEX9651984.1"
                     /translation="MVIFPNAKINLGLRVTEKRNDGFHNLETCFFPVPYFDVLEIIPS
                     KKDSFFIYNSDALTDNILLRARNIFRGFKRFPKVDIFLYKNIPVGSGLGGGSSDAAFL
                     LKGLNTLFNLGIKETKLEEIAEQLGKDCPFFIRNTPSLASGTGNIFESVSVNLSGYYL
                     TIITPGINISTKEAFSWSTPRVPKKLLNETLKNLKPEDWQGLIQNDFQSIIEEKYPEI
                     KEVSETLRKAGSVFTLLSGTGSSVYGLFQRKPKFESLPQSWKVWEGKL"
     gene            complement(4455..5267)
                     /locus_tag="VGA21_15595"
     CDS             complement(4455..5267)
                     /locus_tag="VGA21_15595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017732592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9651985.1"
                     /translation="MAKNVLQGKKGIIFGALDQNSIAWKAAERIYDEGGVFTLSNAPV
                     AMRMGKINELGEKCKAVVIPADATSVEDLENLFTKSMETLGGKLDFILHSIGMSPNVR
                     KGKDYGDLNYDWYLKTLDISGVSFHKVMQVAEKKDAMNEWGSILALTYIAAQRTFPDY
                     ADMAQAKAVLESVARSYGYRFARLKKVRVNTISQSPTMTTAGSGVPGFDVFFDYSDKM
                     SPLGNASAADCANYIVAMFSDYTRMVTMQNLFHDGGFSNSGITEELVQKLSK"
     gene            complement(5377..5889)
                     /locus_tag="VGA21_15600"
     CDS             complement(5377..5889)
                     /locus_tag="VGA21_15600"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020603110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HEX9651986.1"
                     /translation="MLKIELVKGDITNLLVDAIVNAANNSLLGGSGVDGAIHRAAGPE
                     LLAECRTIGGCTTGDAKITKGYKLPAKYVIHAVGPVWRGGENDERMKLKSAYENSLLL
                     AIDNNVKSIAFPNISTGIYNFPKREAAEIAINTVREVLDKNNYSIEVTFVCFDQENYD
                     HYLNLLSDLR"
     gene            5966..6778
                     /gene="murI"
                     /locus_tag="VGA21_15605"
     CDS             5966..6778
                     /gene="murI"
                     /locus_tag="VGA21_15605"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013453708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEX9651987.1"
                     /translation="MSETSIKPVGIFDSGIGGLTVAHAIKELLPMETQVYFGDTAHLP
                     YGDKSKAAIQAYSIKIADVLLQKGCKTIVMACNSASSSAFELLEEYTGSKAKIINVID
                     PMVDYVAENFSGEKVALIGTKRTIESQAYEKKIRKADPTIKLKSVPTPLLVPMIEEGF
                     FNNKISHDIIDEYLGHQVFRGIKALILGCTHYPLIKKQIDNYFNGEVAVLDSSEVVAI
                     HLKKELSCHGLLSPQKTGNDHFLVSDYTRSFESTAELFFREKIHLEMYKLWE"
     gene            6848..8731
                     /locus_tag="VGA21_15610"
     CDS             6848..8731
                     /locus_tag="VGA21_15610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018620252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEX9651988.1"
                     /translation="MSDIIKHECGIALLRLRKPLSYFIEKYGSPTYGMNKMFILMEKQ
                     RNRGQDGAGIANIKIDVPPGHRYISRNRSFDEQALTQIFKKIGKKYKKAKKDGGDDFY
                     NEKWLKEHCAFTGELWLGHVRYGTYGKNSLEYVHPFLRQNNWRSKNLVLAGNFNMTNV
                     DELFKILVGLGQSPKEMVDTVTVMEKIGHFLDEENERIFQKYKKDYSKKEITELIENE
                     CDLQRVLKKSCKDFDGGYAMAGLTGYGGAFVARDPSGIRPAYYYADEEVVVVASEKPA
                     IKTAFNIEFNQIHEIEPGHALIINKSGDFAEFPFIKKLEKKSCSFERIYFSRGTDPDI
                     YTERKNLGRLLVPQILKSINFDLKNTIFSYIPNTAETAYLGLLKGLENYLIARRKEII
                     LDEKPSVDSLEEILSFRPRVEKLVLKDVKMRTFIADDEHRDEIVAHVYDSTYEVVRKN
                     VDTIVVLDDSIVRGTTLEKSILRMLSKLEPKKIVIVSSAPQIRYPDCYGIDMSKLQEF
                     VAFRAAMELIKETGQENILEEVYQKCMDPKEAKKENFVKKIYEPFPYNEISRKIAEIV
                     KPKDMNAELEIIYQTVDNLHKAIPNHSGDWYFTGDFPTPGGNKVVNRAFANFMEGKIV
                     RAY"
     gene            complement(8814..9140)
                     /locus_tag="VGA21_15615"
     CDS             complement(8814..9140)
                     /locus_tag="VGA21_15615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018343551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HEX9651989.1"
                     /translation="MITVTENAKKRLNDLRHQEGKDEGFSVRVSVKGGGCSGLMYDLS
                     FDNKIGDKDEIFEDKGIKIMVDKKSILYLFGTILDFSGGLNGKGFQFINPNASRTCGC
                     GESFAV"
     gene            complement(9147..9554)
                     /locus_tag="VGA21_15620"
     CDS             complement(9147..9554)
                     /locus_tag="VGA21_15620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018478386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651990.1"
                     /translation="MESVSDINVELAKIQNAIQAHLGLDANSLIFDYRTQDGKVRLDL
                     VTINPRHNQSFLYHTEIGFDKLDALKKMQGYVKNAHDKESHYTIQWAARSSKELHTSY
                     FRASNVYEALDKLYYGRDMNTITVFSVNLNPVS"
     gene            9647..10051
                     /gene="mce"
                     /locus_tag="VGA21_15625"
     CDS             9647..10051
                     /gene="mce"
                     /locus_tag="VGA21_15625"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018478389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HEX9651991.1"
                     /translation="MRKVEHIGIAVKNIQNSNQLFQRLLGVDPYKSETVESEAVTTSF
                     FKVGNTKIELLESTTEDSAVAKFIANRGEGIHHIAFEVEDIQKEMARLASEGFQLLNP
                     EPKRGADNKLVVFLHPKSCNGVLVELCQEIKP"
     gene            complement(10075..10251)
                     /locus_tag="VGA21_15630"
     CDS             complement(10075..10251)
                     /locus_tag="VGA21_15630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651992.1"
                     /translation="MLKKIAIFTILFAFLLAGCGPRPQYKTRSGKKKLKNYNSLQYNK
                     SDWDKAKKKPKRKN"
     gene            10334..11371
                     /gene="thiL"
                     /locus_tag="VGA21_15635"
     CDS             10334..11371
                     /gene="thiL"
                     /locus_tag="VGA21_15635"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018473735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HEX9651993.1"
                     /translation="MGESRTELSELGEFGLIDHINQSVKKENQDTLVGIGDDAAVIDC
                     QNENLLLTTDMLVEGIHFDLSYFPLHHLGYKAVACNVSDIAAMNGIPKQMLVSIALSN
                     RFSIEAVDAIYAGINSACEKYKVDLVGGDTTSSSSGLIISASILGRCSKEKTVLRSGA
                     SNKDIICVTGDLGGAYVGLQVLEREKEEFLVNPAMEPRLEKYNYIVGRQLKPEARMDI
                     IHELSDLNVKPTSMIDISDGLASELFHLWRASGLGARIFEGQLPIDKMTYDTAVEFNL
                     DPITCALNGGEDYELLFTIRQEDHEKLKNHRDIHFIGYMESAIDTPTLVTKGENEVPL
                     KAQGWNHFRKH"
BASE COUNT         3606 a         2216 c         2372 g         3228 t
ORIGIN      
        1 cacagtgaca gcggatcccg aacagaccaa aaactacggc tcatccgatc ccgtacttac
       61 ctaccaggta actcctgctt tgattggtgg agatactttt accggagtcc ttgaacgtgc
      121 agcaggagag gatgtagata cgtatgagat caacaacggg acattagatg gcggaggtaa
      181 ttatacgatc acctttgaaa tcagtaattt caccatcacg cccaaaactc tctctgtcac
      241 aggcatcacc atttccaata aagaatacga cggaaccacc gatgccataa taagcggaac
      301 tcccacattg gaaggaatag taggcagtga cgttgtttcg ctgggatcgg gcagtttatc
      361 ggcaaacttc gaagatgcca acgcaggatc gggtaagaac gtaaccgttt cgggcatcac
      421 cctgacagga accaatgcag ggaattacac cctttcaaca actttacccg ttactgccaa
      481 tataactccc cgagccatcg cggtaacagc caatacaaat caaagcaaaa cttacggctc
      541 atccgatccg atactgacct accaggttac cccttcttta atttccggag atacttttac
      601 aggagcattg tcgcgagggt caggagaaaa tacaggcacc tattctatca caatgagcag
      661 cttgagtgcc ggtggcaatt atactataac atttgtgggt gaagacttta ccatcacacc
      721 cctggccatt acggtaactg ccaacccaaa ccaaaacaag gtctttggtt cgactgatcc
      781 ggccttgaca tatagcgtga acccttccct ggttgcagga gattccttta ccggacagct
      841 tagccgcgtt gtcggagaga atgcaggcag ctatgccatc tcaattggca cactttcggc
      901 cgggagcaat taccaaaccg tttttgaatc tggaaacttc attattgaaa aagcatccca
      961 caccatcacc tttgatctaa tcgaggaccg attcaacacc agcgaaccgt ttaccgtaaa
     1021 tgctacggcc ggttcgggat tagaagtgat gttcaatatt ctttccggtc cggcaacagt
     1081 gaatggcagc agcgtaacgc ttaccgggga aacaggaacc gcaacgatcc gcgcctccag
     1141 tgaagatgaa aaccatttac cggctactgc agatcaaagc tttgagataa cgctggatcc
     1201 tgttttggga atggaagaaa cctggaagag agacatcgtt atcttcccgg ttcccagtga
     1261 tggaatgatc accatccggt cgggagagca ggaaatgcaa aaagtcttcc tgctggatat
     1321 cagtggaaag ctgatttgga aaagcagcca ggagaaaccg gtcagggaaa tggaaatcga
     1381 aatccaggag aaaggagaat tcctggtgtt cattcaaacc ggtaaaggaa tgatttcaag
     1441 gaagataatt aaatactaaa acctttttaa atccgcaaaa ggttgagaat aacagttgat
     1501 aagtttaatc agttaaatat cattactatg agaaagtgca tttcggttat aatcgttttc
     1561 ttaataatag gaggatgcga agatgactac ccaattaaaa aggggaacat tcaattctcc
     1621 ttctcatcct taagcggatc tggcggcagg atggagacgc tgaccaagcc tgtagcagtt
     1681 ctgttgagca tcgaagataa tgatgggaat tcaattgaac agaataagaa gatacttcta
     1741 tatacatttg gtaatggtta cgtgagtgaa agcttgcaat tggcaaccgg tacatatcag
     1801 ctcgctcagt tcatggtgct cgatgcagac aacaaagtga tctatgctac accggttgaa
     1861 ggatcggaac tggctcaata tgtaaatgac ccgttgccga ttgagtttgt agttagtgat
     1921 gatggaacaa cattggttac cccgcaggta ttagcggttg atgccgacaa cacaccggag
     1981 aattttgggt atgtgagttt ttcgtttgag gtggtttctc cgttgccaag actaacccaa
     2041 attaaagtat ttgagggagg tctatcgtat gctacacaca tgcttgatta tacctattcc
     2101 aataatgtat tagatgaagt aaagttttac tactgctacc ctgatgaaca gaattgcaat
     2161 acgagtgcct ggtacaaatt tgaatattat ccggatggtc gtatcaaagg aagatatctt
     2221 tatgacgcaa tcactgggaa tgtagtagaa acagatattt atgaatatga aggtattttg
     2281 acgaaaaaga ttaatcatta ttatgaccaa tacaatacat ccaatgcagt tgaatttttc
     2341 tatgatcaga acaacaaatt gactcactca actttaagct caggatactc atctgaaaac
     2401 tctaattatc gtctaagcga atacacgcac aatgaggatg gatctataac cgagcacgtc
     2461 taccttgatg gcctcaataa agatgggtta aggtttattt ttacctatac ttttgaccaa
     2521 tacaataccc cctccttata caataccgga attgcggaat gggttatgtt tcctgtaaat
     2581 aaaaacgttg cttccattac gttaaaggaa atttaccaag ccggtgatcc gccacctgat
     2641 tgttcaacta accaagcata ccaatataat gcgttaggtt taccagagag ctatgttaca
     2701 tccaatcctg aaaactgtta cttaggattt atggatggta cggtagtgca atatttttat
     2761 aaccaatgac agtgaattca tggattctat gcgtggagga ttaatcttaa aaaattaagc
     2821 gttagtccgg attgtaaaac gaaaccccgt agtaatatag taatgacaat ctaagatgat
     2881 agaaactgag cgactaattt taaaaccttt aacatacaac caacttataa aatatattaa
     2941 ggcagataac tctttggaag ttgaattaga tctaaatgaa tcatcaagga caatttcccc
     3001 tgaacttaaa gaagccttag aacaaactat tttgccaaat gttgcagaca taaccaaaga
     3061 ttacttatac tcaacacttt ggactgtaat ttcaaaagaa gctaacaaaa tgattggcga
     3121 catttgtatt gtaggagaac ccaattcaaa cggagaaatt gaaattggtt acggcactta
     3181 tgacgaattt cagaaaaaag gttttatgac agaagccgtt ggtggaatga ttaaatgggc
     3241 aaaatctcaa accaaagtat tttctataat tgcctcgact gaaaaagaga atattgcttc
     3301 gtttacaatt ttgttgaaaa acaattttat taaagttggg gagacagaag cattatttat
     3361 ttggaaactt acacttaaat aattgacaga aataaatcaa tttgaaatac ctaaaacata
     3421 aataaacaac aaaccctagt acggctggga tcgggtgaag atgtgattaa aatctacctg
     3481 agtgatttta gaacacagga tgatggtcat actttctatg ccttgcctcc gcctcttttg
     3541 taaaatcagt ctgagtaggc cccggttaat atttttttct ttgccttcgg ctttcaaaac
     3601 tttaccctat cctttggcaa atcgcgggaa acggatttcc gtttaattat agtttaccct
     3661 cccagacttt ccaactttgt ggtaatgatt caaattttgg ttttctctga aataatccat
     3721 acacagaaga cccggttcct gaaagcagtg tgaaaactga accggctttc ctgagcgttt
     3781 ctgaaacttc cttaatctcc ggatattttt cttcaattat ggattgaaaa tcattttgaa
     3841 tcagaccctg ccaatcttca ggttttaaat ttttgagtgt ttcattcaac aatttttttg
     3901 gaactctcgg agtagaccat gagaaagctt cttttgtaga gatgttaata ccaggcgtta
     3961 ttatggttaa ataataaccg gaaagattca ctgagacgga ttcaaaaata ttcccagtcc
     4021 cagaggctaa tgagggggta ttcctgatga aaaaagggca atccttacca agttgttccg
     4081 caatttcttc cagtttagtt tctttgattc ccagattgaa taatgtgttc aggcctttca
     4141 gaaggaaagc cgcgtcggaa gatccaccac ccaaaccgga acccacagga atgtttttat
     4201 aaaggaagat gtccacctta gggaaccgtt tgaaaccgcg aaagatattt ctggctcgta
     4261 agagaatatt atccgttaat gcatcggagt tataaatgaa aaaggaatct ttttttgaag
     4321 gaataatttc caaaacatca aaatagggga cagggaagaa gcaggtttcc aaattatgaa
     4381 atccatcgtt tcttttttcg gtaaccctta agccaagatt aatcttcgcg ttgggaaaga
     4441 ttaccatcgg agtcttattt actcaatttt tgaacgagtt cctctgtaat acctgaattt
     4501 gaaaatccgc catcgtggaa aagattttgc atagttacca tacgggtata gtcggaaaac
     4561 atggcaacaa tataattggc gcaatcagcg gcgctggcat tgccaagcgg agacatctta
     4621 tcggaataat caaagaatac atcaaatccg ggaacaccgc tgccggccgt tgtcattgtt
     4681 ggagattgag aaattgtatt tacacgcact ttcttcagtc tggcaaaccg gtatccataa
     4741 ctcctggcaa cggattccag aactgctttt gcctgtgcca tatccgcata atcgggaaaa
     4801 gttctttggg ctgcaatata agtgagagcc aaaatcgagc cccattcatt cattgcatcc
     4861 ttcttttcgg caacctgcat cactttatga aaagatactc ctgaaatatc cagcgttttt
     4921 aagtaccagt cataatttaa atctccgtag tctttaccct ttctgacgtt gggactcatc
     4981 cctatggaat gcaggataaa atccagcttg ccaccgagtg tttccatgga tttcgtgaag
     5041 agattttcaa gatcctctac tgatgtagca tcggccggaa ttaccacagc tttgcatttc
     5101 tctcccaact catttatttt acccattcgc atagctaccg gtgcattgct cagggtaaat
     5161 actccccctt catcatagat tctttccgca gctttccaag cgattgaatt ttgatccaat
     5221 gcaccgaaga taattccttt cttaccttgc aaaacatttt ttgccatgcc atgaggtttt
     5281 tagatgccat gcaataatac gaaaaaaaga ctaacatttt agttaataaa acttcggcaa
     5341 aaatgctatt ttgacttaac caggtaacat ttactctcat ctcaggtcac tcaaaagatt
     5401 taaatagtgg tcataatttt cctgatcaaa acaaacaaaa gtaacctcaa tggaataatt
     5461 attcttatcc agtacttctc tcaccgtgtt gatcgcaatt tcggcagctt ctcttttggg
     5521 gaaattgtaa atcccggtgc ttatgtttgg aaaggcaatg gatttaacat tattgtctat
     5581 tgccaacaat aacgagtttt cataagcgga cttcaatttc attcgctcat cgttctctcc
     5641 gccacgccaa acagggccaa cagcatgaat tacatatttg gcaggcagct tgtatccctt
     5701 tgtaattttg gcgtcacccg tcgtacaccc tccaatggtt ctacattcag ctaacaactc
     5761 tggtcctgca gctctgtgta tggctccatc aacaccacta cctccaagca aagaattatt
     5821 ggcagcattc actattgcat caaccaggag gtttgttatg tcgcctttaa ccaattcaat
     5881 tttcagcatt aaacctaaaa taataaaata atttccaatg ggccggttat aatccaaatc
     5941 ctttattttt ggccaaataa aaccagtgag cgaaaccagc ataaaaccag tgggaatttt
     6001 tgacagcggt ataggcggtt taaccgtggc gcatgctatc aaggaattat tacccatgga
     6061 aacacaggta tattttggag atactgccca cttgccttat ggtgacaaat cgaaagcagc
     6121 gatacaggcg tattcaataa aaatcgcaga tgttttgttg cagaaaggtt gcaaaactat
     6181 tgtgatggcc tgcaattctg catcttcctc cgcatttgaa ttgctggaag agtacaccgg
     6241 aagtaaggca aagatcatta atgtaatcga tcctatggtt gactatgtag cggaaaattt
     6301 ttctggtgaa aaagtggcat taatcgggac caaacgaacc attgaatcac aggcttatga
     6361 aaagaagatc agaaaagcag atccaaccat caaactaaaa tcggttccga cacccctgtt
     6421 ggtcccaatg attgaagagg gtttctttaa taataagatt agtcatgata ttattgatga
     6481 atacttgggt caccaggttt tcaggggaat taaagcactt attttaggat gtacacatta
     6541 tccactgata aaaaagcaaa ttgataatta cttcaatggg gaagttgctg tacttgattc
     6601 ctcggaagtt gtggcaattc acttaaaaaa agagctgagt tgtcatggtc tgctaagtcc
     6661 tcaaaagacc ggcaatgatc acttcctggt ttcagattat acccgatcat ttgaatctac
     6721 cgccgaatta ttctttaggg agaagattca cctggagatg tacaagcttt gggaataatt
     6781 ccgtttttca tcaatggcac ttgccagggc ataaaattgt cgtaattttg cagcagacac
     6841 aaatttgatg agtgatatca ttaagcatga gtgcggaatt gcccttctca gactgagaaa
     6901 accactttct tattttattg aaaaatatgg ttcaccgact tatggaatga acaagatgtt
     6961 tattctgatg gagaagcaac gcaacagggg tcaggatggt gcaggaattg ccaacattaa
     7021 aattgatgta cctccgggtc acaggtatat aagccgaaat cgctcttttg atgagcaggc
     7081 attaacacag atttttaaga agatcgggaa gaaatacaaa aaagccaaaa aggatggagg
     7141 ggatgatttc tataatgaaa aatggctaaa ggagcattgc gcgtttaccg gtgaattatg
     7201 gctcgggcat gtgcgttatg gtacttatgg caaaaacagt ttggaatatg tgcatccttt
     7261 tctgaggcag aataactgga gaagtaagaa tctggtgttg gcaggcaatt tcaacatgac
     7321 caatgtggat gaattgttta aaatactggt tggccttggt caaagtccaa aggaaatggt
     7381 agataccgtt acggtgatgg agaaaatcgg acattttctg gatgaagaaa atgaaagaat
     7441 cttccagaaa tataaaaagg actacagtaa aaaagagatt actgaattaa tcgaaaatga
     7501 atgcgatttg cagcgcgttt tgaagaaatc ctgtaaagat tttgacggcg gatatgccat
     7561 ggccgggtta acgggttatg gaggagcatt tgtggcgcgg gatccatccg gcatcaggcc
     7621 agcttattat tacgctgatg aggaagtagt tgttgtagct tccgaaaaac ctgcaattaa
     7681 aaccgctttc aatattgaat tcaatcaaat tcatgaaatc gaaccaggtc atgcattaat
     7741 cattaataag agtggtgatt ttgccgaatt tcctttcatc aaaaaactgg aaaagaaatc
     7801 gtgcagcttt gaacggatat atttcagcag gggaacggat ccggatattt atacggaaag
     7861 aaaaaatctg ggcagattac tggttccgca gattctgaaa agcatcaact ttgatcttaa
     7921 gaacacaatc ttctcctaca taccaaatac tgctgagaca gcctacctgg gcttgttgaa
     7981 aggactggag aattatttga ttgccagacg taaagaaatt atcctggatg aaaaaccaag
     8041 cgttgattct cttgaagaga tactttcgtt ccgtcccagg gtcgaaaagc ttgtgttgaa
     8101 ggatgttaaa atgagaactt tcatagcaga tgatgaacat cgtgatgaaa tcgttgcaca
     8161 tgtatacgac tctacttatg aagttgtaag gaagaacgtg gatacaattg ttgttcttga
     8221 cgattcgata gttcgcggaa caacattgga gaaaagcatt ttgagaatgc tgtctaaatt
     8281 ggaacctaag aagattgtga ttgtaagttc tgctcctcaa atcagatatc cggactgcta
     8341 tggaatagac atgtctaaac ttcaggagtt tgtagctttc cgggcggcaa tggaattaat
     8401 caaagaaaca ggccaggaaa atattctgga agaagtgtat caaaaatgca tggatcccaa
     8461 agaagcaaag aaggaaaatt ttgtgaagaa aatttatgag ccttttccat ataatgaaat
     8521 cagcaggaag atagcggaga ttgtgaaacc taaagacatg aacgcggagt tagaaatcat
     8581 ttatcaaacc gtagataact tacataaagc gattcccaat cattcaggcg actggtactt
     8641 tacaggtgac tttccgactc ccggcggcaa caaggtagtg aaccgcgcat ttgccaattt
     8701 tatggaaggg aagatagtga gagcttactg aacagtgaac agtgagacag tgagacagtg
     8761 aacagtgaga cagtgaacag tgagacagtg aacagtgaga caggtagcaa tagtcagact
     8821 gcgaagcttt caccgcatcc gcaggttcgc gaagcattgg gatttatgaa ttgaaatcct
     8881 ttcccattca aaccgccgct aaaatccagg attgtgccaa agaggtagag gatacttttc
     8941 ttatcgacca tgattttgat acccttgtct tcaaagattt catctttatc cccgattttg
     9001 ttatcaaaag ataaatcgta catcagcccg gagcatccgc cgccttttac tgaaacgcga
     9061 acactaaaac cttcatcctt gccttcctgg tggcggaggt cattcaatct tttcttagca
     9121 ttttcagtta ccgtaatcat atcaaattaa cttaccgggt tcaaatttac gctaaaaacc
     9181 gtaattgtat tcatatcccg gccgtaataa agcttatcca aagcctcata tacgttactg
     9241 gccctgaaat aactggtgtg caattctttt gaactcctgg ccgcccactg aatagtgtaa
     9301 tgactttctt tgtcatgggc attcttcaca tatccttgca tctttttcag agcatccagt
     9361 ttatcaaaac caatttctgt atgataaagg aatgattgat tgtgcctggg gttgattgtt
     9421 accagatcta accggacttt tccatcctga gttctgtaat caaaaatcaa ggaattggca
     9481 tccaaaccca ggtgcgcctg aattgcgttc tggatttttg ccaactctac atttatatct
     9541 gaaactgatt ccatttgctt ataatcctat aactaaatta attcgataaa agtttaattt
     9601 cacaagaaat tgtagataat tgaagttcaa aacgaaattt acccagatga gaaaagttga
     9661 acatattgga atagcagtta aaaatataca aaattctaac cagttatttc aaagattatt
     9721 aggcgttgat ccgtataagt cagaaacggt agaatcagaa gccgtaacaa cctcattttt
     9781 taaggttggt aatactaaaa ttgaacttct ggagagtact actgaagatt ccgcagttgc
     9841 aaaatttatt gcaaaccggg gagaaggaat tcatcatatt gctttcgagg ttgaggatat
     9901 tcaaaaagaa atggcccggt tagcttcgga gggatttcaa ttattgaacc ccgaaccaaa
     9961 aagaggagca gataataagc ttgtcgtatt tctacaccca aaaagttgca atggagtttt
    10021 ggttgaactt tgccaggaaa taaaacctta atagtaatca ttcattagaa ataattaatt
    10081 tttcctttta ggctttttct tggctttgtc ccaatcagat ttgttgtatt gaagcgaatt
    10141 ataattcttc aacttcttct tgcctgatct ggtcttatac tgcgggcgtg gtccgcagcc
    10201 tgctaaaaga aaagcaaaaa ggattgtaaa aatggctatc ttcttcaaca ttaactccct
    10261 tgatttatgt aaatcaaagt tactggaatt ataatggact atcaaattat tgaaataacc
    10321 tacctttgtg catatgggtg aatcacgaac agaactttca gaattgggcg aattcgggct
    10381 catagaccac atcaaccaat cagttaaaaa agagaatcag gacacgcttg ttgggattgg
    10441 agatgacgcg gcggttattg attgtcaaaa tgaaaacttg ttactcacca ccgacatgct
    10501 cgtagaagga attcactttg atctgtcgta ttttccgctt catcaccttg gctataaggc
    10561 agtggcttgc aacgtttctg acattgctgc gatgaatgga ataccgaaac aaatgctcgt
    10621 aagtattgct cttagcaacc ggttctccat cgaagcggtt gacgcgattt atgccgggat
    10681 aaattctgcc tgtgagaaat ataaagtaga tcttgttgga ggtgatacca catcaagttc
    10741 ctccgggtta ataatttcag cgtctattct gggaaggtgc tcaaaagaga aaacagtctt
    10801 aagaagtggg gcatccaata aagatattat ctgtgtaacc ggcgacttag gtggcgccta
    10861 cgttgggctg caggttttgg agcgggaaaa ggaggaattc ctggtcaatc ccgcgatgga
    10921 gccgcgactg gaaaagtata attatattgt gggaaggcag ttgaaaccgg aagcgagaat
    10981 ggatataatt catgaactga gtgatttgaa cgtgaagcca acttcgatga ttgatatttc
    11041 tgatggactg gcatcggaac tattccattt gtggcgggca tctggcctgg gagccagaat
    11101 ttttgaaggg cagcttccca ttgataaaat gacctatgat actgcagttg aattcaatct
    11161 ggaccccatc acttgtgcac taaatggtgg tgaagattat gaactgctat ttactatcag
    11221 gcaggaagat catgaaaagc taaaaaacca ccgggatatt cacttcatag gttatatgga
    11281 aagcgctatc gatactccca cattggtaac taaaggagaa aatgaagttc cgctaaaagc
    11341 tcaaggctgg aatcatttta ggaagcacta atcaataggg taggggataa agatgaattt
    11401 tgtaaaatcg ttgtctatca cg
//
LOCUS       DASWHX010000256         5116 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_709793, whole genome shotgun sequence.
ACCESSION   DASWHX010000256 DASWHX010000000
VERSION     DASWHX010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 5116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5116
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_709793"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..187
                     /locus_tag="VGA21_15640"
     CDS             <1..187
                     /locus_tag="VGA21_15640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007659161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651994.1"
                     /translation="RSITGNYTIPEDACTTYKVTYWMLKEFEEDLHIHIHLENNILFP
                     KAIELEEFILHEFDYKA"
     gene            165..551
                     /locus_tag="VGA21_15645"
     CDS             165..551
                     /locus_tag="VGA21_15645"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEX9651995.1"
                     /translation="MNSITRHNALRNLSGEHNSGLILCWKIRQGMSKNIDPARIREYC
                     SHFYKTHLADHFQEEEKFIFPLLGKNHPMIKKALNDHRKLIENFIKEQDNLKAVNYTK
                     KNLEKHIRFEERNFLMRSRKELRTVN"
     gene            complement(719..985)
                     /locus_tag="VGA21_15650"
     CDS             complement(719..985)
                     /locus_tag="VGA21_15650"
                     /inference="COORDINATES: protein motif:HMM:NF024143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEX9651996.1"
                     /translation="MKTETNMNQSNEKRGKKSGAAALKVAGGVLAGAAAGAIAGVLFA
                     PDSGQKTRKKIVDKTKAAAFDAKRQMVDTLKGIGKKKNKPEAKK"
     gene            1355..1735
                     /locus_tag="VGA21_15655"
     CDS             1355..1735
                     /locus_tag="VGA21_15655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9651997.1"
                     /translation="MKRVIRLFIIALLLLASVGVSVTKHYCGDILKSISVNTKPENCC
                     GDKDMPNGCCEDESENYFLDQDIKIQQIIPVLNPPVAEIDNDLNHLLIISNFDVTNRN
                     LNFYYKYPPPFEPELFVKFRSFLI"
     gene            1838..>5116
                     /locus_tag="VGA21_15660"
     CDS             1838..>5116
                     /locus_tag="VGA21_15660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017732293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX9651998.1"
                     /translation="MLNTFIRFFLENKLITGLIVLLFIGWGISTAPFNWKMDWFPRDP
                     VPVDAIPDIGENQQIVYTNWMGRSPQDMEDQITYPLTTALLGVPGVKTIRSSSIFGLS
                     TIYVIFEEEIEFYWSRSRILEKLNSLPAGILPPEVQPALGPDATALGQIFWYTLEGRN
                     PETGEPTGGWDPQELRTIQDYYVGYALTAATGVAEVAAVGGLVKEYQVDIDPVAMKEY
                     NVNVAQIMNAVRKSNLDIGARTIEFNRAEYLVRGLGYIKSIEDLEKAVVTVSNNTPIR
                     VKDIAKVNLGPASRRGGLDKGGAEATGAVVVSRFGANPLEVIENVKLKIEEISPGLPS
                     KILPDGTVSKVTIIPFYDRTGLIKETLGTLEVALEHEILISIIVVIVLVMNIRASIII
                     SSLLPIGVLMTFIVMRYFGVDANVVALSGIAIAIGVMVDVGIVFVENILRHLEMEENK
                     GIRGKQLLNVIYEASTEVASAIMTAVATTIVSFLPVFAMQAAEGKLFKPLAFTKTFAL
                     MASLLLGLIVVPALAHIVFSFRFDRERSRKVWSVIVAGLGVTFLIIYGSVPAIALILL
                     GINNFFENKWPEKFKAWANRVNTLIILMTVVYFLTSTWMPLGAQNTLLVNYLFVIAIV
                     TVVLGSLLIIVHYYPQLLNWCLVNKWKFLLIPILIVIFGITTWQGFDRIWGFAAKGSE
                     AIGWNLKSNSTWQYLSHRFPGVGKEFMPPLDEGSYLLMPTTMAHSGIEENIEVIRKLD
                     AYVAQIPEVESVVGKWGRVNSALDPAPISMFENTINYKPEFMLDENGHRMRFKVDREG
                     NYLLIDGSTYNSLRDGFRKLEKDELISDKKSGEYFRQWRDHITSPNDIWLEIVKSTRI
                     PGMTSAPKLQPIQTRLIMLSTGMRAPIGLKVYGPDLETIEQVGLELEKILQKVPGIEP
                     ATVFADRIVGKPYLELDINRDQIARYGLTIEDMQRFIEVAIGGIRLTTTVEGRERFPV
                     RVRYAREFRDNPEDVKRVLIPTPAGVQIPLGELAEITYRRGPQMIKSEDTFLTGYVIF
                     DKKDEYAEVDVVEEAQRFIKEKMDNGEFELPAGVSYKFTGNYENQIRAAKRLAVLVPI
                     SLLII"
BASE COUNT         1525 a          978 c         1120 g         1493 t
ORIGIN      
        1 cagaagcatt acaggtaatt atactattcc tgaggatgcg tgcactacct acaaggttac
       61 ctactggatg ttaaaagaat ttgaagaaga tttgcatatc catattcatc ttgaaaacaa
      121 tattcttttc cccaaggcca ttgaattgga agaattcatt ttacatgaat tcgattacaa
      181 ggcataatgc gttaaggaac ttaagcggtg aacacaacag cggattaata ctttgctgga
      241 aaatcaggca gggtatgagc aagaatattg atcctgcacg aattcgagaa tattgcagcc
      301 atttctataa aactcatctg gcagatcatt ttcaagaaga agaaaaattc atatttccgc
      361 ttttgggaaa gaaccatccc atgattaaaa aggcattgaa cgatcacagg aaattaattg
      421 agaatttcat caaggaacag gataatctga aagcagtaaa ttacacaaaa aaaaatcttg
      481 aaaagcatat tagattcgaa gaacggaact tcttaatgag atccaggaaa gaattgagga
      541 cggtaaatta atcgaactta cccggcttat ccgtactcct gacagacatt cggaaatttt
      601 ggatggccaa ttctggaaat agcaaatgtg tttacctcaa attaggtctg ccatccttaa
      661 ctgacattgc cccaaagtag agaacattac gcctggaata gaaagcaata tgaaactatt
      721 acttcttcgc ttccggtttg tttttctttt taccaatccc tttaagcgta tcaaccattt
      781 gcctcttggc atcaaaagcc gctgctttgg ttttgtcgac aatttttttc ctggtttttt
      841 gaccgctgtc cggcgcaaat aaaacccctg caattgcacc ggctgcagcg ccggctaata
      901 caccaccggc cacttttaat gcggcagcac cagatttctt ccctcgtttt tcattagatt
      961 gattcatatt tgtttcagtt ttcattgtta tattttataa agttcagtaa tcttttactt
     1021 ctataagaat aacaataatt gattcatagg tcaatgattt taatcatccc tgattgatga
     1081 taatggattt tgattttacg tgcaatatgg ggcatgggaa ctccggaaac tttctgactc
     1141 ttaaaaagag cacatatctt ataatgatgt aaaagttttc agtaattgta taacacgatt
     1201 tttgagcatc tgggcgttat ttgcactgcc aatggagatt aagagactcc gaaactatct
     1261 ccgggcggga atttataagg aattaaccta ttatttaaag atatttataa accggatttt
     1321 ttactttgcc ttactttaaa ggaataagtt atcaatgaaa agagttatcc gattgtttat
     1381 tattgcgttg ttactgcttg catcggttgg tgtatcggta acaaagcatt actgcggaga
     1441 tatcctcaaa agcatttctg tgaatactaa gcctgagaac tgctgtggag ataaggatat
     1501 gcctaatggc tgctgtgagg atgaatcaga aaactatttc ctggatcagg atataaagat
     1561 tcagcaaatc attcccgtat taaatccacc agtggcagaa atcgataatg acctaaatca
     1621 tttattgatc atttccaatt tcgatgttac aaaccgaaat ttgaatttct actataaata
     1681 tcctccgccc tttgaaccgg aacttttcgt aaaattccgg tctttcctga tttagcatcc
     1741 tgactatttt tagcccttcc ctgggcgaat tctgatctcc aaactgagag ggatgaatca
     1801 tgttgttttt tggatgaagg tttaaaaacc ggtagctatg ttaaatacat ttataagatt
     1861 tttccttgag aataaactga tcacaggtct gattgtcctg ttattcattg gttggggaat
     1921 ctctacagca ccttttaact ggaagatgga ttggttcccg cgcgatcctg ttcctgttga
     1981 tgcaatacct gatattggag aaaaccaaca aatagtttac acaaactgga tgggtagatc
     2041 tccccaggat atggaagatc aaattaccta tccgcttaca acggcattac ttggggttcc
     2101 tggcgtgaag acaatccgga gcagctcaat tttcggactc tcaacaatct atgttatttt
     2161 cgaagaagaa atagaatttt actggagccg aagcaggatt ctggaaaaac ttaactcact
     2221 ccccgcggga attttacccc ccgaagtaca acccgcgctt ggccctgatg ctacggcttt
     2281 gggtcaaatt ttttggtaca cccttgaagg gaggaaccct gaaaccgggg agccaaccgg
     2341 cggctgggat ccccaggaac tgcgtactat ccaggattat tatgtgggtt atgcattaac
     2401 tgcggccaca ggagtagctg aagttgcagc agtaggcggg ttagttaaag aatatcaagt
     2461 cgatattgat ccggttgcca tgaaagagta caatgtgaat gttgcacaaa tcatgaatgc
     2521 cgtgcgcaaa agcaacctgg atatcggtgc caggacgatc gaatttaatc gtgccgaata
     2581 tttggtcaga ggtttgggtt atataaaaag tattgaagat ctcgaaaaag cggtcgtaac
     2641 cgtttctaat aatactccca ttcgggttaa ggatattgcc aaggtaaatt tgggtccggc
     2701 atccagaaga ggaggtctgg ataaaggcgg ggcagaagca accggcgccg tggtagtctc
     2761 aaggtttggt gccaatcctt tggaagtaat tgaaaatgtt aaattaaaga tcgaagaaat
     2821 ttcccccggt cttccctcga agatccttcc ggatggtaca gtttccaaag taactattat
     2881 tccattctac gaccgcaccg gcctaatcaa ggaaacattg ggaacactgg aagtagccct
     2941 ggaacatgag attttgattt caattattgt ggtgatagtt ctggttatga atatccgggc
     3001 ctcaatcata atctccagtt tactgcccat tggcgtccta atgacattca tcgtgatgcg
     3061 ttatttcggg gttgatgcaa atgtggttgc gctctccgga atcgccattg ccattggagt
     3121 aatggtggat gtaggcatag tgttcgttga aaatattctt cgccacctgg agatggagga
     3181 aaataaagga ataaggggca agcaacttct gaatgttatt tatgaagcct ctacagaagt
     3241 ggcatcagca ataatgactg ctgtggcaac tactatcgtc agtttcctgc ccgtgtttgc
     3301 catgcaggcg gccgagggaa aattattcaa accattagcc ttcaccaaga catttgcatt
     3361 gatggcttcc ttactccttg gattgatagt tgttcccgcg ctggcccata ttgttttctc
     3421 tttccgtttc gatcgggaaa ggtccaggaa agtctggtcc gtaatagtgg caggtctagg
     3481 cgttactttt cttattatat atggaagtgt tcctgccata gccttgattc ttttgggaat
     3541 taacaatttt tttgaaaata aatggcctga aaaattcaaa gcgtgggcaa acagagtcaa
     3601 cactttaata atactaatga cagtggtata ttttctcacc agcacctgga tgccattggg
     3661 tgcacaaaat acgcttttgg tgaattacct ttttgttatt gccattgtta cagttgtcct
     3721 gggctcatta cttataatag ttcattatta tccacaatta ttgaactggt gtctggttaa
     3781 caaatggaaa tttctattaa tacccatcct gattgtcatt tttggtatta cgacctggca
     3841 aggctttgac aggatttggg gatttgcagc taaaggttct gaggccatag gatggaacct
     3901 gaaaagtaat tctacctggc aatatctaag tcatagattt cccggcgttg gcaaagaatt
     3961 catgccaccc ctggatgaag ggtcctattt gcttatgcca accactatgg ctcattccgg
     4021 aattgaagaa aatattgagg taattcgtaa acttgatgcc tatgttgcgc aaataccgga
     4081 agttgaatca gtggttggaa agtggggccg ggtcaattca gctttggatc ctgcaccgat
     4141 ttccatgttt gaaaacacta tcaattataa gccggaattc atgctggatg agaatggtca
     4201 caggatgcgc ttcaaagtag acagggaagg aaattattta cttatcgatg gttcaactta
     4261 caacagtctg cgggatggct tcagaaagtt agaaaaggac gagctgattt ctgataaaaa
     4321 aagtggcgaa tatttccggc agtggagaga ccatattacc tcgccaaatg atatctggct
     4381 ggaaatagtg aaatccacta ggatacccgg aatgacctct gctccgaaac tgcagccaat
     4441 ccaaacccgg ttgattatgt tatctacagg aatgagagcc cccatagggt taaaggtgta
     4501 cggtcccgac ctggaaacca tagagcaagt gggattggaa ctggaaaaaa ttctgcaaaa
     4561 ggtaccagga atagagccgg caactgtatt tgcagacagg atagtaggga aaccctacct
     4621 ggaattagac attaaccgtg atcaaatagc ccgttatggt ttaactatcg aagatatgca
     4681 gcgatttatt gaagttgcaa taggcggaat acgattaaca acaaccgtgg aaggaagaga
     4741 acgatttccg gttagggtcc ggtatgcccg ggagttccgt gacaacccgg aagatgtaaa
     4801 gcgagtacta atacctactc ctgcgggtgt gcaaattcct ctgggtgaat tggctgagat
     4861 tacctatcgt cgcggaccgc aaatgataaa aagtgaagat actttcctta ctgggtacgt
     4921 aatatttgat aagaaagacg aatatgcaga agtggacgta gtggaagaag ctcaaaggtt
     4981 tattaaagaa aaaatggata atggtgaatt tgaacttccc gccggagtct cttataaatt
     5041 cacaggtaat tatgaaaatc agattagggc tgctaaaaga cttgcagttt tagtgcccat
     5101 ttcgttgctg ataatt
//
LOCUS       DASWHX010000257        32676 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_2572089, whole genome shotgun sequence.
ACCESSION   DASWHX010000257 DASWHX010000000
VERSION     DASWHX010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 32676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32676
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2572089"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="VGA21_15665"
     CDS             <1..210
                     /locus_tag="VGA21_15665"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEX9651999.1"
                     /translation="LVSVNLLKEQSLEIRFLNTRGQVFNKFIRKMDVTNNSLTLDINF
                     LPQGTYIMQIRTEGKSESFRIIKAQ"
     gene            244..909
                     /gene="rpe"
                     /locus_tag="VGA21_15670"
     CDS             244..909
                     /gene="rpe"
                     /locus_tag="VGA21_15670"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009195367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEX9652000.1"
                     /translation="MSPVISPSLLASDFANLQSEVEMLNRSDADWIHCDIMDGRFVPN
                     ISFGMPVLKAIQKHATKPLDVHLMILEPEKYVEEFRKLGAEIISVHFEACPHLHRNIQ
                     QIKDLGCKAGVAINPHTPAEFLSDVIADIDLVCLMSVNPGFGAQRFIPSTFEKIRRLK
                     SLIREKSSKTLIEIDGGVNMDNAAPLMEAGADVLVAGNFVFNSDNPIQTIHNLKSLSL
                     QER"
     gene            1089..3263
                     /locus_tag="VGA21_15675"
     CDS             1089..3263
                     /locus_tag="VGA21_15675"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEX9652001.1"
                     /translation="MSIGNRLIISHLSYDTLSYIITQNDFQGLKEFFLRPTSKLLEEV
                     TISKGAEHDRNEAGLITLKPVGIESVQSPFGDFSSVLTTLPGVSANNELTASYRVRGG
                     NFDENLIYVNDIPVYRPQLISAGQQEGLSFVHMDMVREVKFSAGGWQSKYGDKLSSVL
                     NVNYKDPNALEGKLIAGLLGGSLYFGYGNPTGKFSLVSGIRHKNARYLLNTFDTKGEY
                     IPQFTDVQGLLRYNISSDRLNRLSILFNTAQNKYEVIPETRETEFGAFGGGTFRFLVG
                     FDGKELLNYTLSQGGIKWDYSINPGWINQLIASGVYSREREISDLESGYRLCDVNTNI
                     GSTNFNECVVIRGLGTQYDYARNNLRVGMLNIENRQSIKLNESNDFEWGISYAYKEIR
                     NKFNEYEFLDSADYVQITEQILNDEEIRVNALEAFFQNETSISGKHAFNYGVRLNFQN
                     LNNNLLISPRLGYSFNPKWRRDIIFRLAAGVYQQQPFLREMIDFNSNFNRKIKPQSSL
                     HIIAGADYNFTWWSRPFKLLSEAYYKYLWNINPYDVDNVRIRYYADNPGEGKVSGVDL
                     RLSGEFIPGTESWFSLSFLSAKENIDGDTRGFIRRPSDQRISMSIVFQDHLPNDPSLK
                     IFLAFAFGSGFPFGPPNDFENRNIFRGEEYRRVDLGLSKSFKLPDKINFDELALRIEI
                     LNLLGTANTISYSWIEDFNGRNFGVPNNLSARFFNVKIEAKL"
     gene            3389..3874
                     /locus_tag="VGA21_15680"
     CDS             3389..3874
                     /locus_tag="VGA21_15680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9652002.1"
                     /translation="MERMIKYLKSIFLLVLGMILSLGAMAQDEEITDEQLRRYALLQE
                     VVDIMKKEISVELNEMIKAQEGMTGARYKELAATKGDEAKMATIGAKDFEKQFFQITE
                     DMKNDRIEAIKTVNQELATKMLGDRGKVYKRIGDALESDAALETRYEAIVAEIKTSSG
                     S"
     gene            4169..4783
                     /locus_tag="VGA21_15685"
     CDS             4169..4783
                     /locus_tag="VGA21_15685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9652003.1"
                     /translation="MFTENEVAAIITFPDINELVDQQRKSFIKAEAPYLEIDNHDFLS
                     LILVTPSLGIALANESVSFFEEMALNKKARKLSKGGYILKKDPVVVAMGFLIKSYDKW
                     AGPFFGVIKSILDKLYNKQDLMTLTKDAAYITDEEFCYLGLKAPFGFIRAITSFFMNE
                     EDEDFIRHRKTNKKDYDRIVLIGEQLGFSEIPFFKKFLTTFDVK"
     gene            4839..5903
                     /locus_tag="VGA21_15690"
     CDS             4839..5903
                     /locus_tag="VGA21_15690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9652004.1"
                     /translation="MRLVKTIVIATFLFFHFMVKGQNPIPMGPYIGQEPKRSIIHTIL
                     SKFRLGIGTGYGRTYYSHKMEDVAFLRKSDTLFIFDPATNISGGSIPAGYTGWFNNPV
                     GHTAFVVGTDTISNPGISIDANDALLSTDTADIKMKSRARTIPLNFTLEFNYQKYRIG
                     IGYQFEFQSMNSFEPKEYADTLIGFTPEFKRATFKRYYLMLGYSFYESGHFKAVADAK
                     IGKMKFGKHYNTSVIQSGIFFNLGVTAEWQFSEYLTAYARPAFEYKSYTVAIPETTYS
                     FNHIYPALFLEVGIKLGLPNLRKCPIVDCATQINHVHWGTEYRSRKHPFWKWQNPHYG
                     ENYKNLIKYKGRNKRKINPY"
     gene            5991..8453
                     /gene="gyrA"
                     /locus_tag="VGA21_15695"
     CDS             5991..8453
                     /gene="gyrA"
                     /locus_tag="VGA21_15695"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011585067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEX9652005.1"
                     /translation="MAEGTNERIIPINIEDEMRSAYIDYSMSVIISRALPDVRDGLKP
                     VHRRVLYGMMDLGLYHNRSYKKSARIVGEVLGKYHPHGDASVYDTMVRMAQPWSLRYP
                     LVDGQGNFGSIDGDSPAAMRYTEARLMRIAEELLADINKNTVDFAPNFDDSLTEPTVL
                     PAKIPNLLINGTSGIAVGMATNMPPHNLTEVVNGTIAYVDNNEITIEELMSYITAPDF
                     PTGGIIYGYQGVREAFLTGRGRILVRGKAEMHQTKTGKEQIIVTEIPYMVNKASMIEK
                     IAGLVNEKRIDGISDIRDESDRDGLRIVFDLKKDAIPNIVLNNLFKYSQLQSSFGVNN
                     ITLVKSKPEMLNLKQLISYFVDHRHEVVIRRAQFELDEAERRAHILQGYLIALDNLDE
                     VIKLIRGSKDPDEAREGLMKQFKLTEIQAKAILEMRLQRLTGLERDKIVKEYEEITAT
                     IADLKDILERVERRMQIIKDELNELKEKYGDKRRTDVVHSADDITVEDMIPDEDMVIT
                     LSHQGYIKRTPLAQYKVQGRGGIGSKGATSKENDFTEHLFIASTHNYLLIFTEYGKVF
                     WLKVWEIPEGGKTAKGRAIQNLINIESGDSVRSVINVKNLNEEEYINNHYIIMCTQNG
                     TIKKTVLEAYSRPRQNGINAITINEGDKLLYVSLTNGENHIVIAVSSGRAIHFHENDV
                     RPMGRTASGVRGITVNKDDMVVGMVCIREMESNLLVVSEKGFGKRSSIEEYRITRRGG
                     KGVKTLNITAKTGKLVAIKEVKDDDDLMIINKSGITIRMEIKDLRVMGRATQGVKVIR
                     LNEDDAISSVEKIEVAEEVTEK"
     gene            8494..9648
                     /locus_tag="VGA21_15700"
     CDS             8494..9648
                     /locus_tag="VGA21_15700"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9652006.1"
                     /translation="MRRLFTLLIMGFITLNSQAQTSAVNKAESAVKAENFVEAKELIT
                     PALEHEKTFDKGRTWYINGLLYKGIALSEDPNVQSIDGDALQKAVDSFKKVMELEKEG
                     TTYHFFADEAINLMWNDIINKGATAYDMDSMDLALTYFLNAKMIKPKDTTSILYTGVV
                     AQFVGNWDMALENYTALVDLGYIKEDVYGSMFHIIRNEKKDNEGAFEILQEAIQHFPG
                     NENFKSTEIEMLIVMEKIDDAKKKLEETIADNPTDPVLYFSLGYMYDESGEAEKAKVN
                     YAKAVELKPDYYEPNINLAILNYNEAIAAYKELNNLSMTDYNKRGPALEAKAKELLKN
                     SLPYWEAAHKSRPDQRTVIENLQNVYAQLKMNDKSEEMRKKLEKLNGNNE"
     gene            complement(9687..10508)
                     /locus_tag="VGA21_15705"
     CDS             complement(9687..10508)
                     /locus_tag="VGA21_15705"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX9652007.1"
                     /translation="MKKYIFFLPILWISCTGSKDETTTSDEPDIVTEEITYTSDTVEM
                     KGFLAYDANQKEKRPGILVVHEWWGQTDYPKERAKMLAQLGYTAMAVDMYGGGQIAEH
                     PQDAGVFSGHVMSDIDGATARFMAALEVLKQHTSVDTERLGAIGYCFGGTVVLTMGRI
                     INELDGVAAFHAGLGIPVPPTSEMNTVFLICNGADDSFVSSESIAKFKMQMDSVGADY
                     RYIDFAGAVHGFTNPGADEYGQKFEIPLAYNSQADLESWEDMKNFFDRLFNENQE"
     gene            complement(10612..11064)
                     /locus_tag="VGA21_15710"
     CDS             complement(10612..11064)
                     /locus_tag="VGA21_15710"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX9652008.1"
                     /translation="MKTMFQFLIATLWLAACSGTPSNNSVQISKAKEEILAAEAAFDA
                     MARNKGVKEAFVSFAANDASLIRGNLVIKGKVAIEEYMNRPSPYTNVKLTWKPDFVDV
                     SVSGDLGYTWGHYTFTALDSLGQSFESEGIFRTVWKKQTDGTWRYVVD"
     gene            11209..16776
                     /locus_tag="VGA21_15715"
     CDS             11209..16776
                     /locus_tag="VGA21_15715"
                     /inference="COORDINATES: protein motif:HMM:NF019323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HEX9652009.1"
                     /translation="MKIKLYKFHLLLISAFILVSCSGEHKTEDTFSSPLSLFVEYISS
                     HTTGIISSRDQIRIHLARESKEADAGKELSVRVFSFDPSIEGKTIWEDKRTIVFQPSK
                     PLPQGKKYNAKFLVKEIFPDADVKEDFKFIFATLEQDFEIQLLGFEITNPNDLNTVNI
                     NGTFQTADFVQESDLEKIISANQNNNTLSISWVHNISQNKHRFLIQNAKRSANSEKII
                     IEVKGKLIGIDRNDETTFEIPALGDYKIISARVINGDDTYASIQFSDPLKADQDLRGL
                     ITLGGNENFRTVVQSNELRIYPRETLTGEIQLKIFEGIRNSAGYQLSANFETSLQFSQ
                     VKPEVRLATNKGTILPSTDGVVLPFQAIGLKEVRVKVTRVFENNILQYLQVNELGRNY
                     QLNRVGRPVASKVVSLVNRGVVDLNKWNRFTLDLKDFVKTEPGALYQVQIFFSKQNTL
                     FTCGEDTKLEITTEEEEGGEIAYQETYWDGYEEYDYDYDYDYDYDYDYDWQQRENPCN
                     SAYYRNRSVTSVLFASDLGLIAKRGEKGSITAFVTNLITTEPVSNASVEFYDYQQQLI
                     ESAATDSEGKVMVDPARPPFLMVVKKDRQFGYLRLLDGAALSVSNFNVTGDKVKRGLK
                     GFIYGERGVWRPGDVINLSFMIEDREKVLPKEHPVILELFNPLNQLVKRMISTGSLKG
                     IYVFNVQTDTEYPTGNWLAKVKVGGAEFTKTVKIETIKPNRLKINLDFPKEKLTVLDQ
                     NITGNLEARWLHGAIAKNLKTEIGLRLVPVNTTFQNYADYEFDDEGKNFYSEPVEVFS
                     GKLNETGAATANISIDVENNSPGALKAIFDTKVYEEGGDFSINQTTIPYYPYKTFVGM
                     KTPEGDRRGIIVTDKDHSVDIVCVDANGNPSPSSQLRVELYKLDWRWWWDNSEELISN
                     YIGRNYNRPILSENLTITNGKGNWTLRLDQPNWGRFYLKVTDINSDHSTGKTIYMDWP
                     GWAGKQRDGMGDATMLVFEPDKPEYKVGESIQIKFPSAKGGHALVSLETGSSVLKTFW
                     AETSDNETTLSIPATEKMSPNVYINISLLQPHGQTENDLPIRLYGIASAKVVDAATEL
                     HPELKMAAELKPGENVNIEVSEKDGKPMAYTIAVVDEGLLDLTNFKTPKPWSHFYARE
                     ALGIKTWDIFDQVIGAYGGRIERILPFGGDDGLKDQEGKETSRFKPVVQFLGPFYLDR
                     NKREKHSFRMPQYVGSVRTMIVAGLDGAYGSVEITTPVRQPLMVLATLPRVAGPEEEM
                     SMPVNLFAMKENVGQVVVKIEASGKLDIQGSAQQTVNFKETGDKVIFFKIKAKPEIGL
                     AKVKVTATAGTVTSVYDIEMEVRPSNPEITDVESEFVESQNSFSFDYSPIGLKGTNHS
                     SLEISSLPPINLEQRLRYLISYPHGCIEQTVSSVFPQLFLENFTEPDEEGKKLVSYYV
                     NLAIQRLQSFQTSDGGFSYWPGQSTVSPWGTNYGYHFLIEAGKKGYFIPASMINGLKN
                     YQTEEANKSSYSSSDPSGSDLNMAYRLYTLALAGSPATGAMNRLRESAYTGTSSKWRL
                     ALAYAVAGLDQAALDLVKDLSMEVKPYRELSNTYGSNFRDEAMILETLVRLNRKADAF
                     KLVEKLALKLSDRYGWMSTQTTAYALIAIAGYVEKNPVGEKVSVEINENGKVFQTISS
                     TFLSQITIVNPEINQKVSVKNSGQGPVYVRLIRSGIPLELDDVDASRNIDFTLNYTTM
                     TGETADVTKLVQGTNFKAIVTVHNPGQRGFYEEIALTQIFPSGWEIINTRLNETGMPI
                     NNDAVPEYQDIRDDRVLTYFDLRQNESKKFTILLNAAYAGKYYQPAVKVAAMYDNEIE
                     ARTSGKWVEVVKE"
     gene            16724..19144
                     /gene="pbpC"
                     /locus_tag="VGA21_15720"
     CDS             16724..19144
                     /gene="pbpC"
                     /locus_tag="VGA21_15720"
                     /inference="COORDINATES: protein motif:HMM:TIGR02073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HEX9652010.1"
                     /translation="MKLRQEHRANGLKLLKSNSTDDANVINRLKLLKTILFIALSILI
                     LVISIPLPKPMFPDEYSTILVDQKGDLLAGSIADDGQWRFPPADSIPDIYKQCVLTYE
                     DEYFYYHPGINPISILKAFYTNMKAGEIKRGGSTITMQVSRMARGNKARTVWEKSIEI
                     LLALKLEIFYSKKDILNLYSNNAPFGGNVIGLSAASWRYFGRSPKQLSWGESATMAVL
                     PNDPAAIFPGRNNLHLLDKRNNLLNKLFSHGIIDSLTLILSKAEPLPGKPKPLPVYAP
                     HLLTRALNDGLKGKVVQSTIDLELQKKVTEKTRIHASNLRANHINNAAALVIDIETGI
                     TLAYVGNIESVPANNDKVDIITAKRSTGSLLKPFLYSLAIDRGIILPKELLPDIPAFF
                     QGFAPQNFDKKFNGVIHADKALTYSLNVPFVYLLQDYGYEQFHRDLQNMGLVNSLNHE
                     PGHYGLSIILGGAESSLWELTSLYAGFYRSMNRTNKIFSEVQDNPYFSNRYLKSSDPE
                     LFAGTEIGRDAIWFTLKTLQELTRPDELSGWQHFESGKLISWKTGTSYGLKDGWAIGL
                     NSKYVVGVWTGNADGEGRPGLTGIRTAAPLMFDIFSLLDGSGNFEQPADLMQTGLICT
                     KSGQKAGAFCDDTRQAFLPKERHSANICQFHQIVHLDEDGEFRVNSGCYPVTSMKHKN
                     WFVLPPVEAWYYKSWTSDYKNLPEIYPGCKEFFLANEYMEPIYPKHRSRIYVPIELDG
                     SPGKAIFEVAHQNKNASLYWHMDEQYLGKTTNNHQMDLHPLKGNHRLKIVDDKGRELE
                     IIFEVISG"
     gene            complement(19214..21673)
                     /locus_tag="VGA21_15725"
     CDS             complement(19214..21673)
                     /locus_tag="VGA21_15725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009280632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX9652011.1"
                     /translation="MKKSTFNGVLTGILTLIYFVSPGQDHLTYQTPDATLLSLVDAPL
                     TPAISVSPDGTYMLLLERPGYPGILELAQPELRLAGLRINPRTNGPSRSGSYTGITIK
                     EIHGTKEIKVTGMPAEARIENVSWSPGSKQFAFTITNDTGIELWTASADSGASDKLTG
                     GVLNDAIGFAPFQWFSDGTNILYKAVIQGRGEAPAKPVVPQGPTIQESTGTKAAVRTY
                     QDLLNDKHDENLFRYYSTSQLHIADTKNKTLKPFGTQGIITQLSPSPDGNFIMINYVD
                     EPFSYIVPYSRFPQTVAVYDRQGKIVSEIAKIPLSEDIPKGFGATREGPRSFTWRNDK
                     PATIYWVEAQDGGDPARETDVRDKLFYLEAPFTGKKKESISLKLRYGGITWGNDNLAI
                     AYESWFSTRQVITSSFKPSDPKSKKVIFDRSYEDRYSDPGNFNTIRNNFGENVLQMDP
                     SGKKLYLIGTGASPEGNRPFVQEFDIASKQASELWRSEAPYYEIAFEILSVEKGLLLT
                     RRESKDEQPNYFVRNIRNGQLTQITQIPHPYPQLKGVQKQVVTYKRADGVDLKGDLYL
                     PAGYKMEQGRLPVLMWAYPAEFKSAEAAGQMDGSPYEFIRISGTSPLLFVARGYAVFD
                     DPSMPIIGEGDKEPNDSFVKQLVMSAEAAVNKLVELGIADRRKIAIGGHSYGAFMTAN
                     LLAHSDLFAAGIARSGAYNRTLTPFGFQREERTYWEAPEVYYNMSPFMHADKINEPML
                     MIHGHADNNSGTFPIQSERMFAAMKGHGGKARLVMLPHESHGYRARESILHVLWETDQ
                     LLENYVKNRPAEEEVKLKVEK"
     gene            22011..22334
                     /locus_tag="VGA21_15730"
     CDS             22011..22334
                     /locus_tag="VGA21_15730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020598883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX9652012.1"
                     /translation="MSKLYLGEFEEMVLTMVAVLGEDAYGNPIVQEFQNRLGREVSLS
                     AVHLTLYRLEDKGLVASRLGGSTQERGGRRKRMYTISSAGVELLRAMKEARSGLWKFI
                     PTLNS"
     gene            22341..25025
                     /locus_tag="VGA21_15735"
     CDS             22341..25025
                     /locus_tag="VGA21_15735"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9652013.1"
                     /translation="MKSTDKRHDIQPPGWAVRILLWLCPEQLSESIIGDLKEQFSSDL
                     KSKGSKTARRRFILNAIKFMRPGILIRRTSHQAMSNTLWYNYFLTAKRNFWRNKTLSA
                     VNIFGIAMGLLSCLFILQYVSYHLSFDSFHENSERIFRITNDRYQDGKLVQHGTITYP
                     TIGPAMAKDYPEIEMFTRIMPSGEITATIGDEITRGGTLFFADQNFFKLFTFSWVAGD
                     LNSALSEPNQIVLSETGAAKIFKVPKDSVSDLIGKSFKLNNRAEYHVVGIFHDMPANS
                     HLQFEAIASYISLYDTENRDADESWVWSDFYHYLLLKPGASAAQLESKFDDFSARNFQ
                     GEKVSGSIEEFYLQSLAEIHLYSDYEYDFAITENGTAVWTLGLIALFILLLAWINYLN
                     LTTSRAIDRVMEISIRKSLGANRYQITNQFIIESLMVCVVAILLSLGSVILLQPYFNE
                     LTALKLSLAGLLKSSGSNLWYIIFLLFAGIILTGIISASFLWTFNLSVVLKGRSNQSV
                     KGRSMRKTLVVVQFSIAATLIIGTLVVSRQLQFMNKANLGISINNRLIIEAPYNSQFD
                     STFISRIDNFKNEIQQLNFVEAAATANRWPGRRLGRGFNIRLSKEPESAGRTMSWMGT
                     DHDYFRTYDIKLLAGRFFAPTDHHRSFNELRSVIVNENAVHNFGFESNDQIIGAEILS
                     FGRPWTVIGVVNNYHQESFHAPMEPILFFPFYGTGHTITIKLKTDDYQNVMSSVKSVY
                     DKFFPGNLFDYFFLEDYYKRQYSEDQQFGDVIVIFTVMAILISILGLMGLASFTAARR
                     VKELGIRKVLGASVIDLVKTLSKDFIILIGIAIFIAIPISYYFMRTWLNDYVYKITLS
                     WELFAGPVIIILGVAVLTIASQIYKAARTNPVETLKME"
     gene            complement(25042..25572)
                     /locus_tag="VGA21_15740"
     CDS             complement(25042..25572)
                     /locus_tag="VGA21_15740"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEX9652014.1"
                     /translation="MDEEMDLSSGEKEAFRKLPRSISIDSKLEDKVVNKLMMNNLIKK
                     KMKTKSYITGIAASVIFFIAGFLLANKIQNPGINDLPSDYMLLLLEDDNFKPTKELPE
                     LVVEYENWLVSQSDAGVKITGAELGYPEVETDYPLVSGFFMIAASSMEEAREIAANCP
                     HKKYGGIVEVREVIKH"
     gene            complement(25576..26091)
                     /locus_tag="VGA21_15745"
     CDS             complement(25576..26091)
                     /locus_tag="VGA21_15745"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX9652015.1"
                     /translation="MQTDDKQLVQRFMKDRSEDSFRMLYRSKSPRLHMTAMRLSANDV
                     NVAEDLIQEMWIRAIQRIETFRWNSSLLTWLTGILVNLNRERFREKVHEKLSEEFSAT
                     QTHKDSGVEVIDLEKAIASLPVGYREILILHDVEGYKHREIAEMLNIDEGTSKSQLFN
                     ARRAMRKYLNS"
     gene            26303..26770
                     /locus_tag="VGA21_15750"
     CDS             26303..26770
                     /locus_tag="VGA21_15750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008201059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX9652016.1"
                     /translation="MAIFNLNVNSTQVQVDVDSDTPMLWVLRDELNLKGTKFGCGIAQ
                     CGACTIHMNGEAVRSCTLSVSAVGEAEITTIEGISVHGDHPVQAAWSEQNVPQCGYCQ
                     PGQIMSAIALLQTHSNPPDSVIDQAMSGNICRCGTYPRIKKAIHSLGEEAEGL"
     gene            26767..28953
                     /locus_tag="VGA21_15755"
     CDS             26767..28953
                     /locus_tag="VGA21_15755"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX9652017.1"
                     /translation="MKTITTTSRREFLKISGVMGGGLIIGFNMYSCKPGEPLPDFIEQ
                     EINAFIRINQDGTATLLSKNPDIGQGVKTSLPMIIAEELDLPWNKVSVEQAPLDVRFG
                     DQFTGGSTGVNMNYTAMRKAGAAAKEVLVKAAANKWKVDPSEIRTENGNLIHGRKKQH
                     YGYFALDAAKLELPEEPTLKNLSEFKLIGTSVSDVDLVQMVTGKPIFGIDQQVDGMVY
                     STVIKPEVFGSKVIAINAAEAKNIPGVIDVFKIEGMDNPALMRDGVAIVANKQWTTFK
                     AKRLVTVEWERPKGYTQSMESMESELLKGLNEGSILREDGDVDSAFDSGKEIMEATYE
                     VPFLSHSQMEPMNFIADVREDEVRLLGPTQSPGGARNLASRITGIEPEKISVEFTRVG
                     GGFGRRLMNDYAGDAVFISNKIKKPVKLIWDRESDFLADYYRPAGAYRFKAILKEKKL
                     DALEVKVCTTSRRLYSGSAENHHETEAFKDQQPAGMIPNFRISYKPIATNIPTGALRT
                     PGVNATTFAYQSFMDELARKVESDPIDFQLNMIGEENRDLPYEDHPGPSYNTGRLKEV
                     IRLVREKSGWDNPAPSGIFRGFAAQMVFGAYVAQVVEISMEDPEGKLKIRKVTAAIDC
                     GIVINPIGADAQVQGGITDAISAALFEEIQLDNGKLVNKNFDKYLKLRMKDSPAVEVH
                     FVRSDAYPSGLGEPAYPVLFPALCNAIFSATGMRIRKLPISRYNLI"
     gene            complement(29113..29235)
                     /locus_tag="VGA21_15760"
     CDS             complement(29113..29235)
                     /locus_tag="VGA21_15760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9652018.1"
                     /translation="MDKAKYEVLKKALVRHLKSKGASTHTEILEAITNNFDNTR"
     gene            complement(29238..29849)
                     /locus_tag="VGA21_15765"
     CDS             complement(29238..29849)
                     /locus_tag="VGA21_15765"
                     /inference="COORDINATES: protein motif:HMM:TIGR02145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FISUMP domain-containing protein"
                     /protein_id="HEX9652019.1"
                     /translation="MYRLLVLILLSLVMNCSGNRNPNESLLQHLNNESVRSKVMLDQM
                     VWMTENLSIEIPGSFCQQDDSLNCIRYGRLYTWEAAKNGCSKLGDGWRLPTNEEWQIM
                     AKWYGGIYDDSDDKGKSAYLNLLDGGNSEFNAYLGGNREANGNYERLGTHGFYWTSTE
                     YESKEAWFYNFAKGSTLLNHHTGDKNRAVSVRCIKDILLEKKL"
     gene            complement(29887..30273)
                     /locus_tag="VGA21_15770"
     CDS             complement(29887..30273)
                     /locus_tag="VGA21_15770"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX9652020.1"
                     /translation="MTNLTHISPFFIVANLKTAVSVYVDKLGFEVRFMGPDDDPFFAM
                     VGRDQISIMLKEIATDVKAIPNHRRHEWARWDAYIYTADPDMLFEEYRSRGVVFRQPI
                     RDDDDGLRGFEVMDADEYVLFFGRPK"
     gene            complement(30277..30633)
                     /locus_tag="VGA21_15775"
     CDS             complement(30277..30633)
                     /locus_tag="VGA21_15775"
                     /inference="COORDINATES: protein motif:HMM:NF024093.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX9652021.1"
                     /translation="MKFEKSNPILYSKDVKNSLKYYTEVLGFEDSWEWGEPTDFGGIV
                     KDGVEVFFCLNAQGNPGTWLAIIVDDVDAYYEIIKEKGAKIISAPKSMEWNMREMLVE
                     DPDCHIIRFGHRIECD"
     gene            complement(30798..31895)
                     /locus_tag="VGA21_15780"
     CDS             complement(30798..31895)
                     /locus_tag="VGA21_15780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019940133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEX9652022.1"
                     /translation="MAKYIYQQKEWTNFTWKDAAINVIFGEVRNLQGKITGQMNALGF
                     STKEEATLTTLTLDVVKSSEIEGEHLNYEQVRSSIARRLGIDVAGLVPPDRNVEGVVD
                     MMLDATQNYLQPLTEGRLFAWHSALFPTGYSGMYKIEVAGYRIGEMQIVSGAMGKEKI
                     HYEAVVPQLVKTEMDKFVNWFNNNDKTDPVIKAAIAHFWFIIIHPFDDGNGRIARAIS
                     DLLLARSENSPERFYSMSSQILVERKRYYEVLQKVQHSSGDITEWLDWFLHCLKNALL
                     ETEDTLKKILRKAEFWKIRENTLLNERQRLVLNKMLDGFDGKLKSSKWAKIAKCSPDT
                     ALRDIKDLIEKRILLQERQGGRSTNYELVEF"
     gene            complement(32281..32475)
                     /locus_tag="VGA21_15785"
     CDS             complement(32281..32475)
                     /locus_tag="VGA21_15785"
                     /inference="COORDINATES: protein motif:HMM:TIGR00305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HEX9652023.1"
                     /translation="MYGEIIDVKSNIELCRDSKDNFLLSLAADSKANFLITGDKDILD
                     LKTIYKVKIITITDYIKRIK"
BASE COUNT        10016 a         6390 c         6804 g         9466 t
ORIGIN      
        1 ctggtgagcg taaatcttct caaggaacag tccctggaaa taagatttct aaacaccaga
       61 gggcaggtgt ttaataaatt tattagaaaa atggatgtaa ctaacaattc tctcacgctt
      121 gacattaatt tccttcccca aggtacttat atcatgcaaa tcagaacgga agggaaatca
      181 gaaagcttta gaataatcaa agcacaataa aattgtgtaa tttagggcat caaacgtaac
      241 tcaatgagtc cggttatttc acctagtttg ttagcttcag attttgcaaa ccttcagagt
      301 gaagtagaaa tgttaaatag aagtgatgcg gactggattc attgtgacat aatggatggt
      361 cggttcgtgc ccaatatttc ctttggcatg ccggtgctta aagctattca gaagcatgca
      421 acaaaacccc tggatgttca cctgatgatt ttggagccgg aaaaatatgt ggaagaattc
      481 cgaaagcttg gagcagaaat tatttctgtt cattttgaag cctgcccgca tttacataga
      541 aacattcagc aaattaaaga cctgggatgc aaagccggag tagccattaa tccacatact
      601 cctgccgaat tcctcagcga tgttattgcc gatattgatt tggtttgcct gatgtccgtt
      661 aatccgggat ttggggctca aagattcata ccttcaacct ttgaaaaaat aagacgactt
      721 aaatctttaa tccgggagaa gagttccaaa actctgatag aaattgatgg gggagtaaat
      781 atggataatg cggccccatt aatggaggcc ggagctgacg tcctggtagc aggcaatttt
      841 gtcttcaact ctgataatcc tattcaaacc atacataatt tgaaaagtct ttcgttacaa
      901 gaaaggtgat ttaccggact cttatttttt tatgcctgtt tgctcccaaa ctaacatttg
      961 ctcaagcgaa tgtacttgag ggacatataa gagatacctt aaaaaatcct gttcagagtg
     1021 cacatattat aataaatgag gtagtaagat cggtttctga ttcaagcggc agttttagaa
     1081 ttactgattt gtcaattggt aatagattaa ttattagtca tttatcatat gatactttaa
     1141 gttatattat aacacaaaat gattttcagg gtttaaaaga atttttcctc cggcctacat
     1201 ctaaacttct ggaagaggtt actatttcta agggtgcaga acatgatcgc aatgaagccg
     1261 gcctcattac acttaaacct gttggaattg aatccgtaca gagtcctttt ggagatttta
     1321 gttccgtgtt gacaacgctg ccaggagttt cagcaaataa tgaattaacg gcgtcataca
     1381 gagtccgggg tggaaatttt gatgagaatc ttatttatgt aaacgacatt ccggtataca
     1441 ggccacaact tatttccgcc ggacaacagg agggactgag ttttgtacat atggatatgg
     1501 taagggaagt gaaattctct gccggcggtt ggcagtctaa atatggagat aaactttcat
     1561 ctgttcttaa tgtcaattat aaagacccaa acgcgttgga agggaagtta atcgcgggct
     1621 tacttggagg gtcgttatat ttcggttacg gaaatcccac cggtaaattt tcactggtca
     1681 gtggaatacg ccacaaaaat gcccgttatc tgttaaacac tttcgataca aagggggagt
     1741 atatcccgca attcactgat gtgcagggat tacttcgata taatatctct tctgatagac
     1801 ttaacagact gtccatcctt tttaacaccg cccaaaacaa gtatgaagta attccagaaa
     1861 cccgtgaaac agaatttgga gcttttggcg gaggcacctt tcgatttctg gttggatttg
     1921 atggaaaaga acttctgaat tatactctat cgcagggtgg gattaagtgg gattacagta
     1981 ttaaccccgg ttggattaat cagctcattg cctcaggagt ttattcccga gagcgtgaaa
     2041 tttcagacct tgaatcaggt taccgcttgt gcgatgtaaa tactaatatc ggcagcacca
     2101 attttaatga atgtgtagtt atacgagggc tgggtacgca gtatgactac gccagaaata
     2161 atctgcgggt aggcatgcta aacattgaga atcgtcagtc aatcaagctg aatgaaagca
     2221 atgattttga atggggaatt tcttatgcct ataaagaaat tagaaataaa tttaatgaat
     2281 atgaatttct tgattctgcc gattatgttc agatcacaga acagatttta aatgatgaag
     2341 aaattcgggt taacgcgctg gaagccttct ttcagaatga aacatcaata tccggcaaac
     2401 atgcattcaa ttacggagtt cggttaaact ttcagaattt gaataataat ttattgataa
     2461 gcccgcggct tggttacagt tttaatccaa aatggcgaag agatattatt ttcaggctcg
     2521 ctgccggtgt gtatcagcag caaccttttc taagggagat gatcgatttc aattcaaact
     2581 tcaaccgtaa aattaaaccc cagtcttcgc tgcacattat tgcaggtgcc gattacaatt
     2641 ttacctggtg gagcagaccc tttaaacttc tgagcgaagc ttattataaa tatctttgga
     2701 atataaatcc ctatgatgtc gacaatgttc gcatccgcta ttatgccgat aatcctggcg
     2761 aagggaaagt gagcggtgtc gacctgcgat tgagcggaga attcattcca ggtacggaaa
     2821 gttggtttag tttgagcttt ctttctgcaa aagaaaacat tgatggggat accaggggat
     2881 ttatacgccg cccatctgac caacggattt ccatgagcat tgtcttccag gatcacctgc
     2941 caaatgatcc aagcctcaaa atattcctgg cgttcgcttt cggttcaggt tttcctttcg
     3001 gaccgccaaa tgatttcgaa aatcgtaata tatttcgtgg tgaagaatac agaagagttg
     3061 acctcggttt gtctaaatct tttaaacttc ctgataaaat taattttgat gaacttgctt
     3121 tgcgaattga aattctaaac ctgcttggaa cggccaatac catatcatac tcatggattg
     3181 aagattttaa tggcagaaac ttcggtgttc ccaataattt atcagcgcgt ttttttaacg
     3241 ttaaaattga ggctaaactc taaaatatac cccaattgca gttatttacg cgaacttttg
     3301 ggtcataaaa acaacctttt ctttgctttt ttttccttaa tgcctgtctt ttgtaggttc
     3361 aataaaagtg ttaaatcgaa tttaaattat ggaacgaatg attaagtatt tgaaaagtat
     3421 ttttttactg gttttgggga tgattttgtc tctgggagcg atggctcagg atgaagagat
     3481 aaccgatgaa caactccgcc gatatgcctt gcttcaggag gttgtagata ttatgaagaa
     3541 ggaaatcagt gtcgagctaa atgagatgat caaagcccag gaaggaatga ccggagcgcg
     3601 gtataaagag ttggcggcca ctaaaggaga tgaagccaaa atggcaacga ttggtgcaaa
     3661 ggatttcgaa aagcaattct ttcaaatcac ggaagatatg aaaaatgata ggattgaagc
     3721 tatcaaaacc gtcaatcagg aacttgctac caagatgctt ggtgaccggg gtaaagtgta
     3781 taaacgaatt ggcgatgccc tggaatcaga tgccgcactt gagacccgat acgaagctat
     3841 tgtcgctgaa ataaaaacca gctccggaag ctagaaatta tttaaaatga ttttattcaa
     3901 gacctgtcaa ccggcgggtc tttttctttc aagtcattcg gggtttaggc ccacgacaat
     3961 tttctgctgt cattcccttt tcaaacatgg gtaattatca aacgcaaccc cgctcgtcca
     4021 gcttgagcag aatctcgttg ctccattgcg aagaatctta ctttcagtgg tacggatgtt
     4081 tgcccttcaa aattgaactt taaccagatt gcattacata ttttgtgttt ttgcctatct
     4141 ttagtgaaca gaaatttgtt ttttacccat gtttactgaa aatgaggtcg ctgctataat
     4201 cacttttcct gacattaatg aattggtaga tcagcagcgc aaatccttta ttaaggctga
     4261 agctccgtac ctggaaattg ataaccacga ttttctcagc cttatcctgg taactccatc
     4321 cctgggaatt gcgctggcta atgaaagtgt aagttttttt gaagagatgg cgctaaacaa
     4381 aaaagcacgc aagttgagta aaggtggcta catattgaaa aaagatcccg tagttgttgc
     4441 catggggttt ctaataaagt cttatgataa atgggcgggg ccattttttg gggttattaa
     4501 atcaatcctg gataaactct ataacaagca agacctgatg accctgacta aagatgcggc
     4561 ttacataacc gatgaagagt tttgttatct tggtttaaag gctccattcg gatttatccg
     4621 cgcaatcact tcctttttta tgaatgaaga agatgaagac tttattcgtc accgcaaaac
     4681 gaataagaaa gattatgata gaatagtgct gattggtgag caattaggct tttcagaaat
     4741 accttttttc aagaaattcc taaccacctt cgatgtcaaa taatgaagag caaaaatacg
     4801 taatgatata tctcttcgtt atctctcatt aaacccaaat gcgactagta aagaccatag
     4861 taattgctac ctttttgttt tttcacttta tggtgaaggg tcagaaccca attcccatgg
     4921 ggccttacat agggcaggaa ccaaagcgaa gcatcattca cactattctc agtaaattca
     4981 gattgggaat tggaaccgga tacggccgaa cttattactc ccacaaaatg gaagacgtgg
     5041 cctttctgag aaaaagcgat acgcttttta tttttgatcc ggcaacaaat atttccggag
     5101 gaagtatccc cgccggatac accggctggt tcaataaccc ggtcgggcat accgcttttg
     5161 tggtgggaac agacaccatc tcaaacccgg ggatatctat cgatgcaaat gatgcgttgc
     5221 tgagtactga tacggccgat ataaaaatga aatccagggc ccgtactatc ccattaaatt
     5281 ttaccctgga attcaactat caaaaataca ggattggaat tggctatcag tttgagtttc
     5341 aaagtatgaa tagttttgaa ccaaaggagt atgcagatac attgattggc tttactccgg
     5401 aattcaaaag agcgactttc aaacgatatt atcttatgct tgggtattct ttttatgagt
     5461 caggtcattt taaagcagtt gctgatgcca aaattggaaa gatgaaattt ggcaaacatt
     5521 acaatacctc tgtaattcaa agcggaatct tttttaatct tggcgttacc gctgagtggc
     5581 aattttcaga atacctgaca gcttatgcaa gacctgcatt tgaatataaa agctatacgg
     5641 ttgctattcc ggaaaccacc tattcattca atcatattta ccctgccttg ttccttgaag
     5701 ttgggataaa attgggatta ccaaacctgc gaaagtgtcc tattgtggat tgcgccactc
     5761 aaattaacca tgtgcattgg ggtactgaat acaggagtcg taaacatcct ttttggaaat
     5821 ggcagaaccc tcactatggg gaaaattata agaacctcat caagtataaa ggacgcaaca
     5881 aacgaaagat aaacccttat taatttctca tggatatgag gaatttcacc aattcgattt
     5941 catcaatttt ttgtatcttt gaggcctgtt tttaacccca atttacgtcc atggctgaag
     6001 gtaccaatga gaggataatt cccataaata ttgaagatga gatgcggagt gcctacatcg
     6061 attattcgat gtcggtaatc atttccaggg ctttgccgga tgttcgggat ggtttgaaac
     6121 ctgtccacag aagggttctt tacggaatga tggatctggg tctgtaccac aacagatctt
     6181 acaaaaaatc agcacgaata gtaggggagg ttttgggtaa ataccacccc cacggagatg
     6241 cgtctgtata tgacacaatg gtaagaatgg cccaaccctg gtcgcttcga tatcctttgg
     6301 tggatggaca aggaaatttt ggttctattg atggtgacag tcctgctgcc atgcgatata
     6361 ctgaagcacg actgatgaga attgcagagg aattgcttgc cgatatcaac aaaaacaccg
     6421 ttgattttgc gccaaacttt gatgactcac ttacagaacc aactgttttg ccggctaaaa
     6481 ttcctaacct gctcataaac ggtacctccg ggatagcggt aggaatggca acaaatatgc
     6541 cccctcataa tcttactgaa gtagttaatg gaaccatcgc ctatgttgat aataatgaga
     6601 taactataga agaactcatg agttatatta ccgcgcccga tttccccacc ggtggaataa
     6661 tctatggata ccagggcgtg agggaagctt tccttaccgg ccggggccgc attttggtga
     6721 gaggtaaagc tgaaatgcac caaactaaga cggggaaaga acaaattata gtcacagaga
     6781 ttccatatat ggtgaataaa gccagcatga ttgaaaaaat tgcaggtttg gtaaatgaaa
     6841 aacgaataga tggaatcagt gatatcagag atgagtctga cagagacggg ctccgaattg
     6901 ttttcgattt gaaaaaagac gctattccca acatcgtact taacaattta tttaagtatt
     6961 ctcaactgca gtcttcattt ggagtcaaca acattaccct ggttaagagc aagccggaaa
     7021 tgctaaatct aaagcaattg atttcatatt ttgttgatca ccggcatgag gtagttatac
     7081 gacgtgcgca gtttgaatta gatgaagccg agcggagggc tcacatttta caaggctacc
     7141 tgattgccct tgataatctg gatgaagtca tcaagttgat ccgcggcagc aaagatccgg
     7201 atgaagccag ggaaggctta atgaaacaat tcaaactgac tgaaattcaa gccaaggcaa
     7261 ttctggagat gcgcttacag cggttaaccg ggttagaaag agataaaatt gtaaaagaat
     7321 atgaagaaat aaccgcgact attgccgatc tcaaagacat tcttgaaaga gttgaacgca
     7381 gaatgcaaat aatcaaggat gagctaaatg agctaaagga gaaatatggt gataagagaa
     7441 gaactgacgt ggtgcattcg gctgatgaca ttactgtaga ggatatgatt ccggatgaag
     7501 atatggtaat caccttaagt caccaggggt atatcaaacg aacacctctt gcgcaataca
     7561 aagtccaggg ccggggaggt atcgggtcta aaggcgccac ttcaaaagaa aatgacttca
     7621 ctgaacattt attcatcgct tccactcata attatctgct gatctttaca gaatatggta
     7681 aagtgttttg gttaaaggtt tgggaaattc cggaaggagg taaaacggct aaaggaaggg
     7741 cgattcaaaa tcttattaat attgaatctg gtgacagcgt cagatccgtg attaacgtaa
     7801 aaaaccttaa tgaggaggag tacatcaaca atcattacat tattatgtgc actcaaaatg
     7861 gaacaattaa aaaaacagtt ttagaagcct atagcaggcc acgacaaaat ggaataaacg
     7921 cgattacaat aaatgaaggg gataaattgc tgtatgtaag tttgactaac ggagaaaacc
     7981 acattgtaat tgctgtgagt tccggtagag ccatccattt tcatgaaaat gatgttcggc
     8041 ccatggggag aactgcatcc ggagtgagag gaatcaccgt aaataaagat gatatggttg
     8101 ttggtatggt atgtatccgc gaaatggaat caaatttgtt ggtggtttct gaaaagggct
     8161 ttggaaaaag gtcttccata gaggaatacc gaattaccag gaggggcgga aaaggggtaa
     8221 aaactttaaa tattactgcc aaaaccggta agttagtggc aataaaagag gtgaaggatg
     8281 acgatgattt gatgattatc aacaaatcag gtatcacaat cagaatggaa attaaagatt
     8341 taagagtgat gggtagagca actcagggag ttaaggtaat tcgcctgaat gaggatgatg
     8401 caatatcatc tgttgaaaaa attgaagtag cagaagaagt aacagagaaa taatttgtac
     8461 tcttaaccat atagtaacaa aagaaaatcg tttatgagaa gattattcac attattgatc
     8521 atgggtttta taacccttaa ttctcaggcc cagacttcag ctgtaaataa ggcggaaagt
     8581 gcagtaaagg cggagaattt tgtcgaagcg aaagaattga ttactccggc gcttgagcat
     8641 gaaaaaactt ttgataaggg caggacatgg tatatcaatg gcttacttta taaaggcatt
     8701 gcactctcag aagatccgaa tgtgcagtct atcgacggtg acgcactcca aaaagccgtt
     8761 gattcattca aaaaagtaat ggaacttgaa aaggagggta ctacttatca tttttttgca
     8821 gatgaggcaa ttaatctgat gtggaatgat attattaaca aaggagcgac agcttatgat
     8881 atggatagca tggatttggc actaacctat tttttgaatg caaaaatgat caaacccaaa
     8941 gacacaactt caatcttata tacgggagta gtagcacaat ttgttggaaa ctgggatatg
     9001 gcattggaaa attacacagc cctggttgat ttaggctata taaaggaaga tgtgtatgga
     9061 agtatgtttc atataattcg aaatgagaaa aaggacaatg agggagcatt cgaaatatta
     9121 caagaagcca ttcagcattt tcccggtaat gaaaatttta aatcgacaga aattgaaatg
     9181 ctgatcgtaa tggaaaaaat tgatgatgcg aaaaagaagt tggaagaaac tattgccgat
     9241 aatccaaccg atcctgtctt atactttagt cttggttata tgtatgatga aagtggagaa
     9301 gctgaaaaag ccaaagttaa ttatgccaaa gcagtcgagc ttaagccgga ttattatgaa
     9361 ccgaacatta atctggccat actgaattac aatgaagcaa tagcagctta taaagaactg
     9421 aataacctga gcatgaccga ttataacaag cgaggtcctg ctttggaagc taaggctaag
     9481 gaattattga aaaactcttt gccttattgg gaggctgcgc acaaatcaag acccgaccag
     9541 cgcaccgtga ttgaaaatct gcagaatgtt tatgcgcaac taaagatgaa tgataaatct
     9601 gaagagatgc ggaaaaagct tgaaaagttg aatggcaaca acgaataata aaaaagggga
     9661 ctagaaggcc ccctttttta ttcagtttat tcctggtttt cattaaataa ccggtcaaag
     9721 aagtttttca tatcctccca cgattccagg tctgcctgtg aattgtaagc taacggtatc
     9781 tcgaatttct gaccatactc atcggcaccc ggattagtaa aaccatgcac agctccggca
     9841 aaatcaatgt atctgtaatc cgctccaaca gagtccattt gcattttaaa cttcgcaatt
     9901 gattcacttg agacgaaaga atcatccgct ccattgcaaa ttaagaaaac agtattcatt
     9961 tctgaagttg ggggaaccgg tattcccaat cctgcatgga aagctgcaac accatccagt
    10021 tcatttatta ttctgcccat agtgagaaca actgtgccgc caaaacaata accgattgca
    10081 cccaatcttt cggtgtcaac agaagtgtgc tgttttagaa cttcgagcgc cgccataaac
    10141 cttgcggttg cgccgtcaat atccgacatt acatggccgg agaaaactcc cgcatcctgg
    10201 ggatgctcag caatttgtcc gccgccatac atatcaactg ccatcgccgt ataacctagc
    10261 tgagcgagca tttttgctct ctctttagga tagtcagtct gtccccacca ttcatgaact
    10321 acaagaattc cagggcgttt ttctttctgg ttggcatcat aagcaagaaa tcctttcatt
    10381 tcaactgtgt ccgaagtata ggtaatttct tcagttacaa tatcgggctc atcagaagtc
    10441 gttgtttcat ctttacttcc ggtgcatgaa atccacaaaa tgggtaaaaa gaatatatac
    10501 tttttcatta ctaaaagatt ttggttgctt cacaaaggta aaaggagcaa caattaatac
    10561 ctaaacgaat caacaccaaa tcagtgcttg atcgtcatat ctaatttaga cttaatctac
    10621 cacgtaccgc caggtgccgt cggtttgctt tttccacaca gtgcgaaaga taccttcact
    10681 ctcgaaactt tggccaaggc tgtctaacgc ggtaaatgta taatgccccc atgtgtatcc
    10741 aagatctcct gagacagaaa catcaacgaa gtcaggcttc caggtaagtt ttacattggt
    10801 gtaaggagag ggcctgttca tatattcctc aattgccact tttcctttga ttaccagatt
    10861 gccccggatc aaggacgcat cattagctgc aaagctgaca aacgcttctt tcactccctt
    10921 attcctggcc attgcatcaa atgcagcttc cgcagcgagt atttcctctt ttgcttttga
    10981 aatttgcacg gaattatttg agggagtacc gctgcatgct gccaaccaga gcgtggcgat
    11041 caggaattgg aacattgtct tcataacatt ttcattttga gtgcaataaa taaggtgccg
    11101 ttaaaattaa gaatccttaa ttactaaaat ttctggttaa gtgcctcaat ctaattgtac
    11161 aaattacata aattgcataa ttatttaaag ttttatccaa atactcttat gaaaatcaag
    11221 ttatataaat ttcatctcct tttaatatca gctttcatcc ttgtttcctg ttcaggtgaa
    11281 cataaaactg aagatacgtt ttcaagtccg ctcagtttgt ttgttgaata catttcttct
    11341 catacaacag gtataatctc ctcaagggat cagatcagaa tccacctggc cagggaaagc
    11401 aaggaagcag atgccggcaa agaattatcc gtaagagttt tttcctttga tccatcgatt
    11461 gagggtaaaa ctatttggga agataaacgc acaattgtat ttcagccatc aaagccacta
    11521 ccacagggga agaagtacaa cgcaaaattc ctggtaaagg aaatattccc ggacgcggac
    11581 gtaaaagaag atttcaaatt tatctttgca acgctggagc aggatttcga gatccaattg
    11641 ctgggtttcg aaataacaaa tccaaacgat ctcaacactg taaatattaa tggcactttt
    11701 caaactgctg actttgtcca ggaatcagat ttagaaaaga ttatcagtgc aaaccaaaat
    11761 aacaatactc tttctatttc atgggttcat aatattagtc agaataaaca ccgctttctt
    11821 attcaaaatg caaagcgttc cgcaaattct gagaaaataa ttattgaagt gaagggaaaa
    11881 ctgattggaa tagatagaaa tgatgaaact acttttgaaa ttccagcctt aggagattat
    11941 aaaattattt cagctcgggt tattaacggt gatgatacgt atgcttccat ccaattttct
    12001 gacccgctaa aagcagatca ggatttaagg ggattgataa cccttggggg gaatgaaaac
    12061 ttcagaaccg tggttcaatc caatgaactt agaatttatc cccgggaaac tttaaccggt
    12121 gaaattcaac ttaaaatatt tgagggtatc agaaattccg cggggtatca gctaagtgct
    12181 aattttgaaa cttcgcttca attttcacag gtaaaacctg aagtcagact tgcaactaat
    12241 aaaggtacta ttctcccaag tacggacggc gtagtgcttc cgtttcaggc tattggctta
    12301 aaggaagtcc gcgtaaaggt aacaagagtg tttgaaaata atattttgca atatcttcag
    12361 gtaaatgaat tagggcgtaa ctaccagtta aatagagtag ggcggcctgt tgcatcaaaa
    12421 gtagtgtccc tggttaatag aggtgttgtg gaccttaata aatggaatcg ctttaccctc
    12481 gatttgaaag attttgtaaa aaccgaaccc ggcgcattgt atcaggttca aattttcttt
    12541 tcaaaacaaa atacactgtt cacttgtggc gaagacacca aacttgaaat tacaacagag
    12601 gaagaagagg gaggtgaaat tgcctatcaa gaaacctact gggatggtta tgaggagtat
    12661 gattacgact atgattatga ttacgactat gactatgact atgattggca gcagcgggaa
    12721 aacccttgca attccgcata ttaccggaac agatccgtaa cctcagtttt gtttgcctcc
    12781 gatcttggcc ttatcgcaaa acgcggtgaa aaaggaagca ttaccgcgtt tgttacaaat
    12841 cttataacaa cggaacctgt ctctaacgcc tcagtcgaat tctatgatta ccagcaacag
    12901 cttattgaaa gcgccgccac cgatagcgaa gggaaagtaa tggttgaccc cgctagacca
    12961 ccatttctca tggtggtaaa gaaagaccgg caatttggtt atttacgact ccttgacggc
    13021 gcggctttat ctgtcagtaa ttttaatgtg accggagata aagtgaagcg agggttaaag
    13081 ggttttattt atggtgaacg aggcgtttgg cggccgggcg acgtaattaa cctttcattt
    13141 atgattgaag acagggaaaa agttttgcca aaagaacatc cggttatcct ggaactattc
    13201 aatccgctaa atcaattagt aaaaagaatg atcagcaccg gatcattaaa aggcatttac
    13261 gttttcaatg ttcaaacaga tacggaatac ccaaccggca attggcttgc taaggttaaa
    13321 gtgggagggg cggaatttac caaaaccgtt aaaattgaaa cgatcaaacc aaatcgccta
    13381 aagataaatt tagattttcc aaaagaaaaa ttaactgtac tcgatcaaaa tataaccgga
    13441 aatctggaag ctagatggct tcatggggca atcgccaaaa acctgaaaac ggagattggg
    13501 ctcagactcg ttccggttaa caccactttt caaaattatg ctgattatga atttgatgat
    13561 gaaggtaaaa atttctactc ggaacctgtt gaagtattct caggcaaatt aaatgaaacc
    13621 ggagctgcca ctgctaatat ttcaatagat gtcgaaaata attcacctgg tgcgctgaag
    13681 gctatcttcg atacaaaagt ctacgaagaa ggtggtgatt tttcgatcaa tcaaacaact
    13741 ataccctatt atccatataa aacttttgtt gggatgaaaa ctccggaagg cgacaggaga
    13801 ggaatcattg tgacggataa agaccattct gttgacattg tttgtgtgga tgccaatgga
    13861 aatccaagtc cttccagcca actgcgagtg gagttataca aactcgattg gagatggtgg
    13921 tgggataact cggaggaatt aataagcaat tatattggca gaaactataa tcgtcccatt
    13981 cttagtgaaa atctaaccat tacaaatggg aaaggtaatt ggactctgag actcgatcag
    14041 cctaattggg gaaggtttta tctaaaagta acagatatta attccgacca ctccaccggc
    14101 aagactattt atatggattg gccaggctgg gctggtaaac aacgtgatgg aatgggtgac
    14161 gctaccatgc ttgttttcga acctgataag ccagaataca aggtaggtga atccatccaa
    14221 atcaaatttc catccgcaaa aggcgggcat gcactcgtaa gcctggaaac aggttcttca
    14281 gttttgaaga cattctgggc tgaaacatcg gacaatgaaa caactcttag tatccctgcc
    14341 actgaaaaaa tgagtccgaa tgtgtatata aatatttccc ttttgcagcc acatggtcaa
    14401 acagaaaatg acttgcccat caggctttat ggaattgctt cggccaaagt agtggatgca
    14461 gccactgaac ttcatccgga actaaaaatg gcagccgaat taaagcctgg agaaaatgtg
    14521 aatattgagg tctctgaaaa agacggaaaa cccatggcat atacaattgc cgtagtcgat
    14581 gaaggtttgc ttgacctaac gaatttcaaa actccaaaac cgtggtcaca tttctatgcg
    14641 cgcgaagctt taggaattaa gacctgggac atattcgatc aggtaatcgg tgcatatgga
    14701 ggcagaatag aacgaattct tccctttgga ggtgatgacg gtcttaaaga tcaggaaggg
    14761 aaagaaacga gcaggtttaa accggtcgtt caatttttag gaccctttta cctcgatcga
    14821 aataagcgag aaaaacattc tttcagaatg ccccaatatg taggaagcgt tcgaactatg
    14881 atcgttgcag gccttgatgg cgcgtatggt tcagtggaga tcacgacacc ggtgagacaa
    14941 cctttgatgg ttcttgccac actgccaaga gtggctggac cggaagaaga aatgtcaatg
    15001 ccggttaatc tctttgcaat gaaagaaaac gttggacagg tggtggtaaa aattgaagca
    15061 agcggtaaac tggatattca gggttccgcc caacaaactg tgaatttcaa agaaaccggg
    15121 gacaaagtaa tattctttaa aataaaggca aagccggaaa taggtctggc taaggttaag
    15181 gtcaccgcta ctgcaggtac tgtcacttcg gtatatgata tagaaatgga ggtgcgacca
    15241 tcgaatccgg aaataaccga tgtggaaagt gaatttgttg aatcgcaaaa ttcattctct
    15301 tttgattatt caccaatcgg actcaaaggg acaaatcatt cttctttgga aatctcttca
    15361 cttcctccca taaaccttga acaaagatta agatatctga tctcataccc tcatggttgt
    15421 atagaacaaa cggtttcatc ggtatttccg cagttgttcc tggaaaattt tacggaaccc
    15481 gatgaagagg gcaaaaagct ggtaagctat tatgttaacc tggccataca acgtttacaa
    15541 tcatttcaaa ctagtgatgg agggttttct tattggccgg gtcagtctac ggttagtcca
    15601 tggggaacaa attatggata ccattttctt attgaagcag gaaagaaagg atatttcatt
    15661 cctgcatcaa tgattaatgg tttgaagaat tatcaaactg aggaggcaaa taaaagcagt
    15721 tatagcagtt ctgatccatc aggttcagat ttgaatatgg cttaccggct ctatacactc
    15781 gcactggccg gtagtcctgc aaccggagca atgaacagac tgcgagaaag cgcatacaca
    15841 ggaacaagct caaagtggag gcttgctctt gcctatgctg tcgcaggatt ggatcaggct
    15901 gcacttgatc tggtaaaaga tctttctatg gaagtgaagc cataccgtga attaagcaat
    15961 acctatggct caaatttccg ggatgaggct atgatccttg aaaccctcgt taggcttaat
    16021 cggaaagcgg atgcattcaa attggttgaa aaactggcac taaaacttag cgatcgatat
    16081 ggctggatga gtacacaaac taccgcttat gctttgattg ccatcgcagg ttatgtggag
    16141 aaaaatccgg ttggtgaaaa agtcagtgtt gagatcaatg agaatggcaa ggttttccag
    16201 acaataagca gcacttttct ctcacaaatt actatcgtaa atcctgaaat caatcaaaag
    16261 gtttctgtga aaaattcagg ccagggtccg gtatatgttc gtttgataag atccgggatt
    16321 cctttagaac tggatgatgt ggatgcaagc agaaatattg attttaccct taactacacc
    16381 acaatgacgg gtgaaacagc agatgtcaca aaattagtgc aaggaacaaa ttttaaagcc
    16441 atagtaactg tacataatcc cggtcaaaga ggtttctatg aagaaattgc attgacacaa
    16501 atatttcctt ccggctggga gattattaat acccggctaa acgaaaccgg catgccgatt
    16561 aacaacgatg ccgttcctga ataccaggat atccgggatg acagagtcct gacttatttt
    16621 gatcttcggc aaaatgaatc aaaaaagttt acaatattat taaatgctgc ctatgccgga
    16681 aaatattatc aaccagctgt aaaagtagca gcaatgtatg ataatgaaat tgaggcaaga
    16741 acatcgggca aatgggttga agttgttaaa gagtaattct acagatgatg ctaatgtaat
    16801 aaaccggttg aaactgttaa aaaccatatt atttattgca ctttccattc tcatcttggt
    16861 gataagtatt ccattgccaa aaccaatgtt ccctgacgaa tattcaacca tcttagtaga
    16921 tcagaaaggt gatcttctgg ctggttcaat tgcggatgat ggtcagtgga gatttccccc
    16981 agcggattcc ataccggata tttataaaca atgtgtttta acatatgaag atgagtactt
    17041 ctactaccat cctggcataa accctatttc tattttaaaa gctttttaca caaatatgaa
    17101 agccggtgaa ataaaaagag gaggaagtac aatcacaatg caagtatcca gaatggcgag
    17161 gggaaataaa gcgcgaactg tatgggagaa gagtattgaa attttattgg cgttaaagct
    17221 ggaaatcttt tattcaaaaa aagacatatt aaatctttat tcaaacaatg caccgtttgg
    17281 tggtaatgtt attggcctgt cagccgcttc ctggagatat tttggcaggt cgccgaaaca
    17341 attatcctgg ggagagtcag caacaatggc cgtactccct aatgatccgg ctgcaatttt
    17401 tcccggaaga aataatttgc atcttcttga taaaagaaat aacctgctca ataaactatt
    17461 ttcacatggc ataatcgatt ctctgacttt aatattgagc aaggcggagc cattaccggg
    17521 taaacccaaa cctttgcctg tttatgcgcc tcatcttctc acccgcgcac taaatgatgg
    17581 tttgaaggga aaagtggtgc aatcaaccat tgatttggaa ttgcagaaaa aagtgacgga
    17641 aaagacccgg atccatgcct ccaacttgcg ggcaaatcat atcaataatg cagcggcatt
    17701 ggtaattgac atcgaaacag gaattacact tgcctatgtt ggaaatatcg aaagcgtacc
    17761 agcaaataat gataaagttg atattattac tgcaaagaga agtacaggca gcctgcttaa
    17821 gccttttctt tattcccttg ccattgatcg cggaataatt cttccaaaag aacttcttcc
    17881 ggatatccct gctttcttcc agggatttgc cccgcaaaat ttcgataaga aatttaatgg
    17941 agtgatccat gctgataagg cactcactta ttcactgaat gtgcctttcg tttatttgtt
    18001 acaggattat ggctatgaac aatttcatcg cgaccttcaa aatatggggc tggttaattc
    18061 ccttaatcat gaaccaggtc actatggact gtccattatt cttggggggg ctgaatcaag
    18121 cctttgggaa ctgacttccc tttacgccgg gttttaccgg agcatgaatc gaactaataa
    18181 aatattttct gaagttcagg acaaccctta tttcagcaac cggtatctaa aatcttcaga
    18241 tccggaatta tttgcaggaa ccgaaatagg ccgcgacgcg atttggttta cattaaagac
    18301 cctgcaggaa ttgacccgtc cggatgaact ttccggctgg cagcatttcg aatctggaaa
    18361 actcatttcc tggaaaaccg gaacaagcta tggactaaag gatggatggg caatcggatt
    18421 aaatagcaaa tatgtcgtgg gtgtttggac aggaaatgcc gacggagaag gaagacctgg
    18481 ccttaccggg attagaacag cggcaccgtt gatgtttgat atctttagtc ttcttgacgg
    18541 gtccggaaat tttgaacagc cggccgactt aatgcaaacc ggattgatat gcacaaagag
    18601 cggacaaaaa gcgggtgcat tttgcgatga caccaggcag gcattccttc caaaagaaag
    18661 acactctgcc aatatttgcc agtttcacca aattgtccac cttgatgaag atggagaatt
    18721 cagggttaac agcggctgtt accctgtaac ttcaatgaaa cacaagaatt ggtttgtgct
    18781 gcctccggtg gaggcctggt attataaaag ttggacatct gattataaga atcttcctga
    18841 gatttatcct ggctgcaaag aattttttct tgcaaacgaa tatatggaac cgatctatcc
    18901 caagcatcga tcaaggatat atgtacccat tgaattggac ggatcacccg gaaaagcaat
    18961 ttttgaagtt gctcatcaaa ataaaaatgc atccctttac tggcacatgg atgagcaata
    19021 tctgggtaaa acgaccaata atcatcaaat ggatttgcat ccattgaaag gaaatcacag
    19081 gcttaaaatt gtggatgaca aagggaggga gctggaaatt attttcgaag tgattagcgg
    19141 ataataagaa accccaggcc tgctaaaagc ctggggtcaa agtcaagtta tggcttttaa
    19201 tttgacttta tttttatttt tctactttca acttcacttc ttcttccgct ggacggtttt
    19261 tcacataatt ttctaaaagc tgatccgttt cccacaacac atgcagaatg gattccctgg
    19321 ctctgtagcc atggctctca tgaggcagca tcaccaaacg cgctttgcca ccatgacctt
    19381 tcattgctgc aaacattcgt tcactttgaa tagggaaagt tcccgagtta ttatcagcat
    19441 gtccatgaat catcagcata ggttcattaa tcttgtcggc atgcataaaa ggagacatgt
    19501 tgtaatacac ttccggtgct tcccaataag ttctttcttc cctctgaaat ccgaaaggag
    19561 ttaaagttct gttgtacgca ccacttctgg caatacctgc cgcaaacaga tcagagtgtg
    19621 ctaataagtt tgctgtcata aaagctccat aagagtgacc accaatggca atctttcgcc
    19681 tatcggcaat tcccaattct accagtttgt ttacagcagc ctcagcactc attaccagtt
    19741 gtttcacaaa ggaatcattt ggctctttat cgccttcacc aataatgggc atgctgggat
    19801 catcaaatac cgcatagcct cttgccacaa aaagcagtgg cgaagtaccg ctaattctaa
    19861 taaactcata aggtgaacca tccatttgac ctgcagcctc cgcactttta aattcagctg
    19921 gataagccca catcaaaaca ggcagccgac cctgttccat cttgtaaccg gcaggcaagt
    19981 acaaatctcc tttcaaatca acaccatcgg cgcgtttgta agtaactacc tgcttttgca
    20041 ctccctttaa ctgcggatag gggtgtggta tctgagtgat ttgggtgagt tgcccattcc
    20101 tgatatttct aacaaaataa ttaggttgtt catccttaga ctccctccgg gttaacaata
    20161 aacctttttc aacacttaag atctcaaaag cgatttcgta atagggagct tctgatctcc
    20221 aaagctcact tgcctgtttg gaagcgatat caaattcctg gacaaatgga cgattaccct
    20281 cgggtgaagc accagtccct ataagataaa gtttctttcc ggacgggtcc atctgtagaa
    20341 cgttttctcc aaagttattc ctgattgtat tgaagtttcc cggatcagaa taccggtctt
    20401 cataagacct gtcaaagatt acttttttgc tttttggatc cgaaggtttg aaagaactcg
    20461 ttattacctg gcgagttgaa aaccaggatt cgtatgcaat ggccaggttg tcattgcccc
    20521 aggtaattcc accatatcgc aacttcaggg aaatgctttc tttcttcttg ccggtgaacg
    20581 gagcttccag gtaaaacaac ttatctctaa catcagtttc tcttgccgga tcaccaccat
    20641 cctgagcttc tacccaataa attgtcgccg gcttatcatt gcgccaggta aaacttcttg
    20701 gaccttctcg tgtagcccca aatcccttgg gtatgtcttc ggacaaagga atttttgcaa
    20761 tctcacttac aattttaccc tgacggtcat aaaccgcaac cgtctgtgga aatctactat
    20821 agggaacaat ataagagaat ggttcgtcca cataatttat cattataaaa tttccatccg
    20881 gagaaggtga caactgggta ataatacctt gggtaccgaa tggctttagg gtcttgtttt
    20941 ttgtgtctgc aatatgcaat tgagaagttg aatagtaccg gaacaaattt tcatcatgct
    21001 tatcatttag caagtcctga taagttctta cagcagcttt agttcctgtg ctttcctgga
    21061 tagttggacc ctggggtacg acaggttttg caggcgcttc accgcgaccc tggattacag
    21121 ctttataaag aatattcgtt ccatcactga accattgaaa gggagcaaag ccgattgcat
    21181 cattcagaac acccccggtg agtttatcag atgcacccga atctgccgag gctgtccaaa
    21241 gttctattcc ggtatcattg gttatagtga aagcaaattg cttgctgccc ggagaccaac
    21301 ttacattttc aattcttgct tcagccggca tcccggtaac tttaatctct tttgttccat
    21361 gaatttcttt aattgtgatt ccggtatagc tgccacttct gcttggtccg tttgtgcggg
    21421 gattgattct taacccggca agtcttaatt caggttgagc cagttccaga attcccggat
    21481 atccgggacg ctccagcaat aacatatatg tgccgtcggg actaactgaa attgccggcg
    21541 ttaatggagc atcaaccagg ctcaacaatg ttgcgtcagg cgtttggtag gtaagatgat
    21601 cctgaccggg tgaaacaaaa tatattaggg tcagaatacc cgttaagact ccattaaatg
    21661 tagacttctt catgataact ctattaaagt atcggaaata gaaaagaatt gtaatctact
    21721 ataatgtaag gagttataaa agggtaaatc cacgataacc ctgtacagaa acggacagaa
    21781 acagtctatc agcctgtaaa cgtgccattt ggaaagaaga ttttttttga aaaaatttca
    21841 tacaaatcta ttatggattt ggcctcttaa taaaaccaaa ttcctactta aaattccact
    21901 attttttgac gatttatgtg ggattattgt gatatgaatt ggagttttga atgatttatt
    21961 ttacaattta tgaacaaata ctgtacattt gttttacaat ttatataata atgagcaaat
    22021 tatacctggg agagtttgaa gaaatggtcc tgacaatggt tgcggttctg ggcgaagatg
    22081 cctacggcaa ccctattgtt caggagttcc aaaacaggtt aggccgggag gtaagtttaa
    22141 gtgctgtgca tttgacgttg tatcgtcttg aagacaaagg gttagtggca tccagacttg
    22201 gtggatcaac ccaggaaaga ggcgggagaa ggaagcggat gtacacaatc tcctccgcgg
    22261 gagttgaact gcttcgcgca atgaaagagg ctaggtcagg actttggaaa tttattccga
    22321 cgctaaattc gtaaacccaa atgaaatcaa ctgacaagcg acatgacata caaccgccag
    22381 gctgggcagt ccgcatactg ctgtggcttt gtccagaaca actgagtgaa tctattattg
    22441 gagacctaaa ggaacaattc agttccgatt tgaaatcaaa aggctcaaag actgcccgca
    22501 ggagatttat tctgaatgca attaaattta tgaggccggg aattctcata agaagaactt
    22561 cccatcaggc aatgtcaaat actttatggt ataattactt cttaaccgca aaaagaaatt
    22621 tttggagaaa caaaacttta tcagcagtaa atatttttgg cattgcaatg ggattgctgt
    22681 cttgtctctt cattttgcag tatgtttcct accatttgag ctttgattcc tttcatgaga
    22741 attcagaaag gatattcaga attaccaatg atcgttatca ggatggaaaa ttagttcaac
    22801 acggaactat tacctaccca accattggac ctgcaatggc gaaagattat cctgaaatag
    22861 aaatgttcac gagaattatg ccatccggag aaattacagc caccattgga gatgaaatta
    22921 cccgcggagg aacgctattc tttgctgatc aaaatttctt caaacttttc accttttcat
    22981 gggttgccgg tgatctcaat tccgcattgt cggagccaaa tcaaattgtg ctgtcggaaa
    23041 caggagctgc taaaatattc aaggtcccaa aagactccgt ttccgactta attgggaaaa
    23101 gcttcaagct caacaacaga gctgagtacc acgttgtagg tattttccat gatatgccgg
    23161 caaattctca tctccagttt gaagcaattg catcatatat atcactctat gatacagaaa
    23221 acagagatgc ggatgaaagt tgggtctggt cagatttcta ccattatctt ttattaaagc
    23281 caggagcaag cgccgcgcaa ctggaatcaa aatttgatga tttcagtgcc aggaattttc
    23341 aaggcgaaaa ggtttccgga agcattgagg agttttacct gcaatctttg gcggaaattc
    23401 atctctattc ggactatgaa tatgattttg caatcactga aaatggaacc gcggtatgga
    23461 cacttggcct tattgcattg ttcattcttt tgcttgcatg gatcaattat cttaacctga
    23521 ctacatcccg cgcgatagac agggttatgg aaattagtat caggaaatct ctgggggcga
    23581 accggtatca aattacaaat cagtttataa ttgaatcatt gatggtctgt gtggtcgcta
    23641 ttctcctgtc cctgggatct gtaattcttc ttcaacctta ctttaatgaa ttaacagcgc
    23701 ttaaactttc tctggccggt cttctgaaaa gcagcggaag caatctttgg tatattattt
    23761 ttcttttatt cgccggaata attcttacag gaattatttc agcatccttt ctatggacct
    23821 ttaacctttc ggttgttttg aagggcaggt caaaccaatc ggtaaagggc aggtcaatgc
    23881 gcaaaacttt ggtggtggtt cagttttcaa tcgcggcaac actaattatt ggaaccctgg
    23941 tagtaagcag acaattgcag tttatgaata aagccaatct ggggatttca ataaacaata
    24001 gattaattat tgaggcgcca tacaattctc aatttgacag tacatttatt tcaaggatcg
    24061 ataatttcaa aaatgaaata caacaactca attttgttga agcggccgcg actgcaaatc
    24121 gatggccggg acgaaggttg ggaaggggat tcaatataag gctttcaaaa gaacctgaat
    24181 ctgcgggaag aaccatgagt tggatgggaa ctgatcacga ttatttcaga acctacgata
    24241 ttaagttact tgcaggccgt ttttttgctc caactgatca tcaccggtct tttaatgaat
    24301 tgagatcggt aattgtaaat gaaaatgccg ttcataattt tggctttgaa agcaatgacc
    24361 aaattattgg cgcagaaatt ttgagttttg gccgaccgtg gaccgttatt ggtgtggtta
    24421 acaactatca ccaggaatca tttcatgctc ctatggaacc tatacttttc ttcccatttt
    24481 atggaaccgg acatacaatt acaatcaaac ttaaaacaga cgattatcag aatgtaatga
    24541 gttctgtaaa atctgtttat gataaattct tcccgggaaa ccttttcgat tatttttttc
    24601 ttgaggatta ttataaaagg caatattcgg aagatcaaca atttggagat gtaatagtaa
    24661 tatttaccgt catggcaata ctaatttcaa ttttaggact gatgggactc gcgtctttta
    24721 ccgcagcaag acgcgttaag gaacttggaa ttagaaaagt tttgggcgcc tcggttattg
    24781 atcttgtcaa gacattgagc aaagatttta ttatactgat aggaattgca attttcattg
    24841 caattcctat atcctattat tttatgcgaa cctggttaaa tgattacgta tataaaataa
    24901 cgctcagctg ggaattattc gcaggtccgg taattataat cctgggagtc gccgtattga
    24961 ccatcgcttc tcaaatttat aaagctgcac gaaccaatcc tgtggaaact ctcaagatgg
    25021 agtaaatatt actccaggac ctcaatgttt aattacttcg cgaacttcaa caattccacc
    25081 gtatttctta tgtggacaat ttgctgctat ttccctggct tcctccatac tcgatgcagc
    25141 aatcataaaa aatcccgata caagaggata atcagtctcc acttcaggat atccaagttc
    25201 cgcacctgta attttcactc cggcatcaga ttgggacaca agccaattct cgtattcgac
    25261 taccaattcc ggcaattcct tggttggctt gaaattgtca tcttccaaaa gcagcaacat
    25321 ataatccgac ggaagatcat taatgcccgg attttgaatt ttattcgcta ataaaaatcc
    25381 cgctataaag aaaattacag aggccgcgat tccggtgatg tacgattttg ttttcatttt
    25441 ctttttaatt aaattattca tcattagttt attaacaact ttatcttcaa gtttagaatc
    25501 aattgagata cttctgggta actttctaaa agcttccttc tctcctgaac tgaggtccat
    25561 ttcttcatcc atagtttaac tgtttagata tttcctcatc gcacgtcttg cattgaacag
    25621 ctgactttta cttgttccct catctatgtt gagcatttcg gcaatttcac ggtgtttgta
    25681 gccttccaca tcatgcagga tcagaatttc cctgtaccca acaggaaggg atgcaattgc
    25741 tttctccagg tcaataactt caacgccgga gtctttatgc gtttgggttg ctgaaaattc
    25801 ctctgataac ttttcgtgaa ctttctctct gaacctttcc cggttaagat tcaccaggat
    25861 tcccgtcagc catgtaagta aagatgaatt ccaccggaat gtctctattc tttgaattgc
    25921 ccggatccac atttcctgga ttaaatcttc cgccacattt acatcattgg cactcaatct
    25981 catcgccgtc atatgtaacc tgggactctt gcttctatat aacattctga atgaatcctc
    26041 ggatcggtct ttcataaatc gctgaacaag ttgtttatcg tcagtttgca ttaattatta
    26101 gtgacatata ggaggaaatg gttgtattgt acaacacttt ttgtgaatct aaaagagata
    26161 tgaagaactg tagtggataa aatagagtat gtctgaaaaa agtatccgct agtcgaaaaa
    26221 agcggccttt caaataacaa ttggaaacta aaattaccaa atccgttaaa ttagaagatg
    26281 ctattaattt aatccggaaa ctatggcaat tttcaatctt aatgtaaact ccacgcaagt
    26341 tcaggttgat gttgattcag atactccaat gttatgggtg ttgcgggatg aactgaatct
    26401 gaaaggaaca aaattcggtt gcggaattgc tcaatgtgga gcatgtacca tacacatgaa
    26461 cggagaagcc gttcggtcat gtactctttc agtttcagct gttggtgaag cggaaattac
    26521 taccattgaa ggaatttctg tacatgggga tcatccggtt caggcagcct ggagcgaaca
    26581 aaatgttcca cagtgcggct attgccagcc cgggcaaata atgtcggcaa ttgccttgct
    26641 tcaaacccac tctaatccgc cagattcggt aattgaccag gccatgtctg gtaatatttg
    26701 ccgttgcgga acctatcccc ggattaagaa agcaattcac agcctaggag aggaggccga
    26761 aggattatga aaacaataac cacaacatca agaagagaat ttctaaaaat tagcggcgtg
    26821 atgggtggcg ggttgatcat tggatttaac atgtatagct gcaagcccgg tgaacctttg
    26881 ccggatttca tagagcagga aattaacgcc ttcataagaa ttaaccagga tggcactgca
    26941 acgctattgt caaaaaatcc tgatatcggc cagggcgtaa aaacctccct tccgatgatt
    27001 attgcggaag aactggatct accctggaat aaagtgtctg tggaacaggc accactggat
    27061 gtccggtttg gggatcaatt tacaggcggc agtacgggag tgaatatgaa ttacaccgcc
    27121 atgaggaaag cgggagcggc agcaaaggaa gttctggtca aagcagctgc aaataaatgg
    27181 aaggttgacc cttcagaaat aagaactgag aatggaaatc taattcatgg caggaaaaag
    27241 caacactatg ggtactttgc attggacgcc gcgaagcttg aacttccgga agaaccaact
    27301 ttaaagaatc tatcagaatt taaactgata gggacctcag tttctgatgt tgatcttgtt
    27361 cagatggtta cagggaagcc aatcttcgga attgatcaac aggtggatgg gatggtgtat
    27421 tcaacggtta ttaaaccgga agttttcggt tctaaagtta tcgcaattaa cgcggctgaa
    27481 gcaaaaaaca ttcccggagt aattgacgtt tttaaaattg agggaatgga taacccggcg
    27541 ctgatgcgcg atggagttgc aatagtggcg aacaagcaat ggactacttt caaagcaaaa
    27601 agattagtaa cggtagagtg ggagagacca aagggttata cccaatcgat ggaaagcatg
    27661 gaatcagagt tacttaaagg attgaatgaa gggagtattc ttcgtgaaga tggtgatgtg
    27721 gattcagcat ttgattccgg caaagaaatt atggaggcta cgtatgaagt tccatttttg
    27781 agtcatagtc aaatggaacc tatgaatttt atcgcggacg ttagggaaga tgaggtcagg
    27841 ttgctaggac ctacacaaag ccctgggggt gccaggaacc tggcctcccg gattaccgga
    27901 attgaaccgg aaaaaatctc cgttgagttt acccgggttg gcggtggctt tggcagaaga
    27961 ctgatgaatg attacgcggg agatgccgtt ttcatttcta ataaaataaa gaaaccggtc
    28021 aagctgattt gggatcggga gagcgatttc ctggccgatt attatcgtcc ggccggagca
    28081 tacaggttta aggcaatttt aaaggagaaa aagctggatg ccctggaagt aaaggtttgc
    28141 accacctcgc gcagacttta ctcaggaagc gcggaaaatc atcatgaaac tgaagctttc
    28201 aaagatcagc aaccggcagg aatgattcca aatttcagga ttagctacaa accaattgcc
    28261 acaaacattc ccacaggtgc actgcgaacg ccgggagtaa acgctacaac ttttgcttac
    28321 cagagtttca tggatgagtt ggcgcgtaaa gttgaatcag atcccattga ttttcagctt
    28381 aacatgattg gtgaagaaaa cagagaccta ccgtatgagg atcatccggg gcccagttat
    28441 aataccggtc ggctgaaaga agtgattcgg ttggttcgtg aaaaatcagg atgggataat
    28501 ccggctcctt ccggaatatt ccggggattc gcagcccaaa tggtttttgg agcttatgtg
    28561 gcccaggtag ttgaaatctc catggaggat cctgagggca agttgaaaat caggaaagtt
    28621 accgcggcaa tagattgtgg aatagtaatt aatcctattg gagcagatgc ccaggttcag
    28681 ggtggaatta cggatgcgat aagcgctgca ttgtttgaag agattcagct ggataatgga
    28741 aaactggtga acaaaaactt tgacaagtac cttaaactta gaatgaaaga ttcgccggcg
    28801 gtagaggtac actttgtaag aagtgacgca tatccctcag ggctgggaga gcctgcttat
    28861 ccagtattgt ttcccgcttt gtgcaatgct attttctccg ccaccgggat gcgaatccgg
    28921 aaattaccca tctccaggta caatttgatt taataaaatt tggaaatacc tttcttttgt
    28981 cgttcggcaa agttcacggt caggtaattg taaattttga tggagatttg tcgtcaagtc
    29041 gtttaatttc tttttttgct tctaagtcaa gtttaaccca ttcgatatac cattcaatag
    29101 agccttcaaa ttttatcttg tattatcaaa attattggtt attgcttcta aaatttccgt
    29161 atgagttgat gcaccctttg atttaagatg ccttacaaga gctttcttca ggacttcata
    29221 ctttgctttg tccatactta cagctttttt tccagcagga tgtctttaat gcatcgtaca
    29281 gaaacggcac gattcttatc accagtatga tggttcaaca gggtagaccc ctttgcgaaa
    29341 ttataaaacc acgcttcttt actttcatac tctgttgaag tccaataaaa tccatgggtt
    29401 ccaagtcgct cataatttcc atttgcttcc cgattaccac ctaagtaagc attgaattcc
    29461 gaattacctc catctaacaa attcagataa gctgatttgc ccttgtcatc tgagtcatcg
    29521 tagatacctc cataccattt ggccataatt tgccattcct cgttcgtcgg taatctccat
    29581 ccatctccaa gtttactgca gccattctta gcggcctccc aggtgtataa gcgcccgtac
    29641 cgaatgcaat ttagtgagtc atcttgttgg caaaatgacc cgggaatttc gatactcaaa
    29701 ttttcagtca tccaaaccat ttggtctaac attactttag aacgaacact ttcgttattt
    29761 aaatgttgca aaagggattc atttggattt ctatttcctg agcaattcat tacaagggat
    29821 aataaaatca agactaatag tctatacatt tcattcggat tttttcttag cgatttatga
    29881 ttgctattat ttaggccgcc caaagaacaa aacatattca tcagcatcca ttacttcaaa
    29941 cccacgtaaa ccatcatcat catcccggat gggctgacgg aaaactacac cgcgagaacg
    30001 atactcctca aacaacatat ccggatctgc ggtataaatg tatgcatccc agcgtgccca
    30061 ttcatgacgt ctatgattgg gaatcgcttt tacatcagtt gcaatttcct taagcattat
    30121 ggaaatttga tcgcgaccaa ccatagcaaa aaagggatcg tcatccgggc ccataaaccg
    30181 gacctcaaac ccaagtttat ctacatagac tgataccgcg gtctttaagt ttgcgacaat
    30241 aaaaaagggt gatatgtggg ttaggtttgt cattgtttaa tcacattcaa ttctatgacc
    30301 aaacctgatg atgtggcaat cgggatcttc taccaacatc tctctcatgt tccattccat
    30361 gcttttaggt gcggaaataa tttttgcccc tttttctttg attatttcgt aataagcatc
    30421 tacgtcatca actataattg ccaaccaggt tcccggatta ccctgagcat tcagacagaa
    30481 aaatacctca acaccatctt ttactatacc accaaaatcg gtcggctcac cccattccca
    30541 tgagtcctca aagcctaaga cttcagtata gtattttaaa ctattcttta catcttttga
    30601 ataaagtatc ggatttgatt tttcgaattt catagtaact taatttatga ttttagtcac
    30661 aggcataaag atatcattgt cttcgttatt tttttcaaag tgggttttat tcacggtttt
    30721 tcaaccacaa aataacaaaa aatagttagg aatgatggag cgaaaagtaa attgaaggct
    30781 ttggagaggg cttgctatta aaattctacc agttcataat tcgtgctacg acccccttgt
    30841 cgttcttgca gtaaaattct tttttctatt aaatccttta tgtcacgcaa tgctgtgtcg
    30901 ggtgagcatt tggctatttt tgcccacttt gatgatttta attttccgtc aaatccatct
    30961 aacattttat taagaaccaa gcgttgtcgc tcatttaata gtgtgttttc gcggattttc
    31021 caaaactctg ctttacgtaa gatttttttc aacgtgtcct ctgtttctaa taatgcattt
    31081 ttcaggcaat gcagaaacca gtctaaccac tcagtaatat cgccactact gtgctgaact
    31141 ttttgcaata cttcataata ccgtttccgt tctacaagta tttggcttga catactgtaa
    31201 aacctttcgg gactgttttc agaacgagca agtaacaaat ctgaaatcgc tcttgcaatc
    31261 ctgccgttac cgtcatcaaa agggtgaatg atgataaacc aaaaatgggc aatggctgct
    31321 tttattaccg ggtctgtttt atcgttattg ttgaaccagt taacaaactt gtccatttct
    31381 gttttaacca attgcgggac aactgcttcg taatggattt tttctttgcc cattgcaccc
    31441 gaaactatct gcatttcgcc tatgcgataa cctgctactt caattttata cattccgcta
    31501 tatcccgtgg gaaaaagtgc agaatgccag gcaaataatc ttccttcggt caatggttgc
    31561 agatagtttt gggtagcatc aagcatcata tccacaacac cctccacgtt cctgtcggga
    31621 ggaactaatc ctgctacatc tattcccaaa cgccttgcaa ttgatgaacg aacctgctcg
    31681 tagttaagat gttcaccttc tatttccgaa gattttacaa catcaagtgt aagcgtggta
    31741 agtgtagctt cttctttcgt tgagaagccc agtgcattca tctgccctgt gatttttccc
    31801 tgcaaattgc gaacttctcc aaatatgaca ttgatagccg catctttcca agtaaagttt
    31861 gtccattcct tttgttgata gatgtatttt gccactgttt gaaaattatc ttgcggcaaa
    31921 tataaggatt attcaacgca taaatgcgta gaatgagtaa aatatttacc gcaaattcaa
    31981 ggcaataaga gttgttttgg atagtctgtc gattgaagta cggctttccc gttagccttg
    32041 tacgccaact ataaggttac tgatggttac cgattcgata ctctgaaggc atggatcaaa
    32101 tctaaaataa ttcgattatt gtaaataccc aagttgaaaa cttctattta tttcagggct
    32161 taagtcaccc acatccagcc cgcaaacccg aaggcttaag ccagagtggg ggaaaagaaa
    32221 aaactaacac cttactttgc atcgttttat catagaaaca ttatatgtcg acgtgcgtgc
    32281 ctacttaatt ctttttatgt agtccgttat tgtaataatt ttaaccttgt aaattgtttt
    32341 taagtcaagt atatctttgt ctcccgttat taaaaaattt gctttagagt cagccgctaa
    32401 tgatagtaag aaattgtctt ttgagtcacg acataactca atattactct ttacatcaat
    32461 tatctctcca tacaatcaaa taagtctaag agctgttcaa cgtcattttt gtcgaagtat
    32521 ttcttgagtt ttggtctatg tgaaactgat aggaattctt ccaggacact caaggctgaa
    32581 aatgattttg atatttccgt tcttaatatt ttcgtctagc ttttttaagt cttttgtaat
    32641 gtaaccctac ggtaaagtac atatttcgag gatgat
//
LOCUS       DASWHX010000258        18447 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_3324050, whole genome shotgun sequence.
ACCESSION   DASWHX010000258 DASWHX010000000
VERSION     DASWHX010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 18447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18447
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3324050"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="VGA21_15790"
     CDS             complement(<1..218)
                     /locus_tag="VGA21_15790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009581604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9652024.1"
                     /translation="MSETVDVKKKTLANPFPGLRPFSIDESHLFFGREGQSDEVLLKL
                     SKNRFIGVIGPSGSGKSSFIYCGVLPIL"
     gene            complement(224..1672)
                     /locus_tag="VGA21_15795"
     CDS             complement(224..1672)
                     /locus_tag="VGA21_15795"
                     /inference="COORDINATES: protein motif:HMM:NF024668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HEX9652025.1"
                     /translation="MAIDSKILILCAAVDNEPITEGETGWVSNFMRFLDMMLFQVLGE
                     KVGIEMKTEKNSISSGDLSAYKLFIPVITPAFLEVGKNRDTVEEFFKIEKGIIFKVTK
                     ARVNRDDQPTKLRNMLGYELFMVDVETGEDHQINDFFGPEAERSYWMKLVDLAYDIHE
                     AILKAKETTAATVKPLFERKSVYLAETSHDLSIQRNIIRRELQRYGYKVLPETTLPVN
                     SGELEETVRKELDECNLSIHLIGNSYGEIPKGSERSVVDLQNKIAGETSANGRKSGKL
                     NRLIWISPDLKNTSERQRTFIENIKRDISALEGAEILQTPLEDFKNIIREELIDVSGE
                     KKFQIGVSRPKIENKGTKMVYLVYDLIDEKEVNPLIKFIEKSGYEVIHPSFQGDLLEL
                     RQKHIENLVEFDLALIYYGKVNLQWVRMKLLDLLKAPGFGRAKPILGRGILTGTGINI
                     NKESFSLYDVDIIDGGKELPLDDIKNFLENKR"
     gene            complement(1681..4863)
                     /locus_tag="VGA21_15800"
     CDS             complement(1681..4863)
                     /locus_tag="VGA21_15800"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX9652026.1"
                     /translation="MKSFLLRALNIEPAEYSQVSYLLALGFSIGIFLASYDVVSVTLF
                     VREFDEATQLPIAVVVGGGIAIVFTFLYSLLQTRVSFRGMAIGWIFLMLLLVAGSYYR
                     IETTAEGSYDRQVIYFTVFTLAVPFNFISLLIFWGSFGRLFNLRQAKRIIGGIDSGQL
                     VASIVALFSIGLLVESLRRTEDLLILSATAVLGFLVTFFMMSGKHQLIVKSSEGKVKL
                     QRLAISKMIKDRYISLMMIFVIISMVAVLFMDYAFVNVAGAQYPGDKEFGSFIAYFEA
                     TVVIFSFLFQTFVTDWIIANYGLKIALIINPILTAILTGLAVLIGFSVGYTADEQWFI
                     YFFLIIAGCKLFIDSLKDALDGPSFKLYFLPIDSAVKFDVQTKIDGLVTAFAGVMAGG
                     VIILMNNFQLQFIYIIIGLIPILIVWYFTTIRMHHNYKETLQLTLNKTKGDVQEEKTN
                     YSINSVLQHEVESEKEDRVIYGLKLMERLDPALFETSILKFLDSDSETIKEFATNSVK
                     SLDLEYEDPEKSEIKELASRAKGMAEMTEVLSIPSSRLHALGTSIKMEDRLLAAKLLR
                     RLIDAENIFILLDLLRDQDPKVRMAAIATARKVKRPETWPVLIELLNSSTYSHAAVAA
                     LVEAGPPCLFTLETAFHRSGQTQKVMLKIVQIMTRIGGDEAFRLMWKKIDFHDRKIVK
                     QILQALRFYHFQANEQQFPVIRDLLDAEIGKAIWNMAAISELPEDENNKNLVDALEEE
                     IASNYDHMYMLLCLLYDPKQVILIKHNIETGTADGIGLGIELLDLFLHPDLKPKLFPL
                     IDNISVNDKVRQLQIFYPREKYSHYEVINYIINRDYNQINRWTRALALKSTENIDDFK
                     VSKGIVAQLFNPDYFLMETAAWIIYKKDKRTYQRVGQRLDETVKRNLDEKLFSTFFFE
                     SFKDDFQLTIDKIFFLKNIPVFSQISGILLSQIVDRFRTLKLKDGAKVEVFDEDNDKP
                     IFIVGKGNVTLYHEDDPLITLKEKDVFGDIFTMDESKSTTSLTVKGETIIFKITLNDF
                     FDVMANQHELAQSFISNISSKFIKQETAKL"
     gene            complement(5134..6336)
                     /locus_tag="VGA21_15805"
     CDS             complement(5134..6336)
                     /locus_tag="VGA21_15805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014022865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HEX9652027.1"
                     /translation="MKDSPILISEFTYDLNESLIANHPASPRDSSRLLFYNRGMIEDK
                     LFKDFPNLLPSNSALFFNNTKVIKARLFFNKPSGAKVEILLLEPVVPAETNQIMNSRN
                     NCVWQCMIGNLKKWKGEPLELIINDSSTQNSKLSIKLINREKQIVEFTWNLHQTFAEI
                     LSGIGNIPLPPYLKREPTENDQLDYQTIYSSVAGAVAAPTAGLHFTKNIFRKLEAKNI
                     ETNYLTLHVSGGTFKPVEVEIIKEHPIHQEEIIIKKETVKSLMREKKVIAIGTTSLRV
                     LESIYWFGVQLGIPGGFVNVIRKETPYQINPVPHPESLQNVLEHMRKSQQDIISIKTE
                     LFIYPGYKFQICHGLVTNFHLPKSTLITLVAAFIGNDWRKVYRHAIENNYRFLSYGDS
                     SMLIPGDE"
     gene            6405..7487
                     /locus_tag="VGA21_15810"
     CDS             6405..7487
                     /locus_tag="VGA21_15810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009282507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HEX9652028.1"
                     /translation="MSLSANYRKRNLKFEFVARTSRGSFREKDSYYIILREGETIGIG
                     EVSPLKGLSVDDVPEIENEIALTLKKMEGLNSREIFPDISEFLKHHVDDRFPSIRFGI
                     ETALMDLKNGGSLIIYPGAFEKSESGIEINGLIWMGDLEFMLTQITTKIYDGFRCIKI
                     KIGSLDFERECDILKYVRNKYFRDKIEIRVDANGAFKPEVALEKLDILFEYDLHSIEQ
                     PIKPGQKDVMKSLCRQTPIPIVLDEELIGIHAQGEMLSLLEFIRPQYIVLKPTLLGGF
                     IRCNQWIECAEKLEIGWWVTSALESNIGLNAICQYTDNLSVKIPQGLGTGELFTNNIP
                     SPLEIKKSEIRLNKYKNWDVSEILKE"
     gene            complement(7465..8154)
                     /locus_tag="VGA21_15815"
     CDS             complement(7465..8154)
                     /locus_tag="VGA21_15815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018343697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HEX9652029.1"
                     /translation="MKKIVVLTGAGVSAESGIPTFRDSGGLWEGHNIEDVATPEGWHR
                     NPEMVLEFYNQRRKAALQANPNLAHELIAKLQDYFEVTVVTQNVDNLHEKAGSKKVIH
                     LHGSIFEARSTHNPSLVYPIKGEIKLGDKCEKGSQLRPNIVWFGEMVPAMEPAIKEAS
                     ASDIFIVVGTSLLVYPAAGLLDYVSRGIPRFIIDPKMPTIAPEDKLFLIEEKATIGMR
                     KVFDTILSEFH"
     gene            complement(8155..10473)
                     /gene="topA"
                     /locus_tag="VGA21_15820"
     CDS             complement(8155..10473)
                     /gene="topA"
                     /locus_tag="VGA21_15820"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017729828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEX9652030.1"
                     /translation="MAKNLVIVESPAKAKTIEGYLGKEYKVASSYGHVRDLPKDDSAI
                     DIENGFQPKYEISKDKKEVIENLKKLVKDAELIYLASDDDREGEAISWHLKEALNLED
                     ARTRRIVFHEITKNAIQNALENPRGIDLDLVNAQQARRVLDRLVGFELSPILWKKIKT
                     GLSAGRVQSVAVRLVVEREREIESFKSGSNFRVQALFKLDDGSELNAELSSRFNSIDK
                     AEDFLNKCLGAEFNIEKLQKKPGKRSSSPPFTTSTLQQEAGRKLGYSVSQTMILAQKL
                     YEAGKISYMRTDSLSLSEDAISNAASRIKQSYGDKYLHIKHYKTKSKGAQEAHEAIRP
                     TDFSDLIGSNDKREQKLYELIWKRAIASQMADAQLEKTTATIGISTTTEKFIATGEVV
                     IFDGFLKVYVESREEEDENSVSSGILPPLRENQVLNMYEIKSREVFSKHPPRYTEASL
                     VKKLEELGIGRPSTYAPTISTIQKRGYISKETRDGKERKYQSFRLRDGKITKSVETEI
                     TGYEKNKLFPTNTAMVVNDFLVENFPTVTNYSFTAEVEKEFDDIASGKKVWNKMLKDF
                     YGNFHERVEFTNTLKKSDVGPMRKLGIDPKSGKPIFARLGKFGPVVQIGTGEEEEEKP
                     RFASMRNGQFIESITLEDAIELFKLPRELGDFEGSKVDVNIGRFGPYVRHEGKFISLP
                     KEDDPYSVELERAIGLIKEKREADSKKLIRSFPENPDIQVLNGRFGPYIKAGSKNVKI
                     PKGKIPEELTLEECLELVEKAPEKKKFIRRKK"
     gene            10597..11109
                     /locus_tag="VGA21_15825"
     CDS             10597..11109
                     /locus_tag="VGA21_15825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009579002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX9652031.1"
                     /translation="MEAELFLIRHAEALDKSPEIKDLDRQLSPIGLRNSSIQGELIKK
                     NKILPDLIVSSNAVRAAYTAHLIAERIGYDTSRIIINDEVYEASVRTLLNVVNQLNDE
                     LNKVFIIGHNPSISYLAEYISKSEIGDILPGSIVRLKRLTDSWSLVSENTCELQSYNY
                     PGETDEINNE"
     gene            11102..12154
                     /locus_tag="VGA21_15830"
     CDS             11102..12154
                     /locus_tag="VGA21_15830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009057121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase family protein"
                     /protein_id="HEX9652032.1"
                     /translation="MSEIENFDPNGVGVKGTLFGLPFNEENASLILIPVPWEVTVSYG
                     AGTAEGPMNILDCSTQVDLFSPQIKDAWKMGIAMLEIDPVIQETSYKLRAEALEYIQE
                     QEMGDDDLGEKRNFSDIPSRVDEGSLQMINWVREKTANILSKEKMVGLIGGDHSTPLG
                     LIEALGSKYSEFGILQIDAHADLRKAYEGFTYSHASIMYNALQNESIGKLVQVGVRDF
                     SEDEYQMIKDSGGRISCFFDSDLRERLGNNESWKTICKEIISELPESVYISFDIDGMD
                     PKLCPGTGTPVPGGLEFDMVTTLIRDLSLSGRKIIGFDLVEVGAHPWDGNVGARLLYS
                     LCNWMGVSQKKLSPAT"
     gene            12225..13574
                     /locus_tag="VGA21_15835"
     CDS             12225..13574
                     /locus_tag="VGA21_15835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020532888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HEX9652033.1"
                     /translation="MESGIVSFANWIWDYPLLILLMGGGFFFVVYSRFLPFKYFGHAL
                     GVLSGKYDKADEVGEINHYEALSTHLAATVGMGNISGVAVAIAVGGPGAIFWMWISAL
                     VGIATKFFTCSLSIMYRGKDSLGATQGGPMYVIVEGLGKRWKPLAVLFCLAGMIGVLP
                     IFQANQLTQAIRDVVLVPNGIGQGGFVSDLITGIVLTILVSVVILGGIKRIATVAGKL
                     VPLMVVLYFVTVMIIIMIDYHDIIPSFALIFEDAFSGNAVMGGAVGAVILTGAKRAAF
                     SNEAGIGTAPMAHGAAKTDEPIREGLVAMLGPAIDTLIVCTLTALAIIITGVWKTTDH
                     DGVTLTMAAFNSALPGFGGYILILCAFIFATTSLFSLSYYGTKCWSFLVGADKTRYYE
                     YFYVISIIIGAVASLTAIISLIDAFYAIMAIPTMTSAVLLAPKVLKEAKRYFSSLPK"
     gene            complement(13575..14150)
                     /locus_tag="VGA21_15840"
     CDS             complement(13575..14150)
                     /locus_tag="VGA21_15840"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX9652034.1"
                     /translation="MKKFVREWIVILGVFGILYATGLHTEVIAFTQRIILSTGIIAPK
                     KLNADKWERAVYDVELKSLSSEKIINLSDYRGKVIFMNFWASWCGPCIAEMPGIQNLY
                     NKTDTTKVKFILISLDSQSGDAIQFLHRKNFDLPAFFPLSPLPEIYNPPAVPTTFIIS
                     PDGMVVSKTIGMADYNKKSFIKYLEELAGKK"
     gene            14320..16113
                     /locus_tag="VGA21_15845"
     CDS             14320..16113
                     /locus_tag="VGA21_15845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9652035.1"
                     /translation="MKRAVALVIVALILSVQNAYSQKVKYKDLFILLNSKKYEEAEPF
                     LRVFMADPKNSDHANAHFHMALMFEFKANRNDILTQNHLLVNNIDSALYYFTLAKNMI
                     DEKETSKNDEYYQTYSRRDLRTGKFGIKLSDIHLEIENKVKVMTGQKSSLTNLFTHYV
                     RTEKYYTDADSVFLILQNKYKNQNNLFLKAGNTDLANLKLLAAKFDSVRYNFNQYKSY
                     LEKFPESKYNQKITIVDVGRLEEVAIMPIDWKDETIKVRNYREWSASTETVITVELKK
                     LKEHLTTYNNQLDKLTEKIVKDSVSAKNELTALIDQMLAEDLKKYDRNPMPLAIFDYK
                     VKKIQYLDLLFQYDFDELYLKDKIIVAKARAKLGEETLAIVKAIAEFNLPEETIKYPE
                     FFQTIGETGGISTLLANGKREMEEAVNKWTLEMEIIMERMNWGISQADSISLVPAIAQ
                     SYKYHTLLVKDSIAEARAIVSGLVSSESKLTFTAIIDEDYEIDTLFAFEMDPFINSVR
                     HEIIPLMQITDLNNILTGFYALKSDTVSRMDLSLISINSVSWTTHLDVPGKISKIIYV
                     PDVEEVKVFIKRNEEDVLALTLDSGGKPKDQ"
     gene            complement(16107..17339)
                     /locus_tag="VGA21_15850"
     CDS             complement(16107..17339)
                     /locus_tag="VGA21_15850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018622119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HEX9652036.1"
                     /translation="MVLNYLWIAFFLIAFIVGLVKLVFFQDTEVFPAIFNSTFDMAKT
                     GFELSLYLTGVMSFWLGIMKIGENGGIIRLLSKAIGPFFSRLFPGIPSNHPAIGSMIM
                     NFSANMLGLDNAATPLGLKTMKEMQEVNPDKDTASDAQIMFLVLNTSGLSIIPLTILI
                     DRSVVGASNPTDIFIPILIATFFSTIVGLITVALYQKINLLDRVILSYLGGITIIIIG
                     LVYYFTSLNQDQLNSVSSVASSVIIFSVIIFFIVLGWAKKVNVYEAFIDGAKEGFQIS
                     IKIIPFLIAILVAIGVFRASGALDYIVSGISFVVAGLGFNSDWVQALPTALMKPLSGS
                     AARGMNIEVMKTFGPDSFVGQLSSVFRGATETTFYVIAVYFGSVSIKKIRYAATAGLI
                     ADLAGIIAAIFVGYLFFH"
     gene            complement(17342..18040)
                     /locus_tag="VGA21_15855"
     CDS             complement(17342..18040)
                     /locus_tag="VGA21_15855"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HEX9652037.1"
                     /translation="MIAFFQPEFLNGVEFLTEEDSNHCIRVLRQTVGDNIEILNGKGA
                     KAVVEIIDANPVKVQFRILHKEIIPQRSYSIHLAIAATKNHERLEWCIEKCTEIGVKK
                     ITIINCRHSERIKIREARLLKKIASAVKQSHNPFLPELKAILPFTQLISSSSEDERFI
                     CYVDKLQPNHLYNLATPNKSYCVVIGPEGDFTQDEIRLAEENDFQKVSLSANTLRTET
                     AGVYAVSALNLINC"
     gene            complement(18044..18304)
                     /locus_tag="VGA21_15860"
     CDS             complement(18044..18304)
                     /locus_tag="VGA21_15860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020526698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA-binding protein"
                     /protein_id="HEX9652038.1"
                     /translation="MLKEEFQKATLEVNSLTTRPSNEDLLQLYSFYKQATDGDITGEK
                     PSGFDFKAIAKYSTWEKLKGMGAEEAMQKYIALVSQLKEKLS"
BASE COUNT         5500 a         3568 c         3631 g         5748 t
ORIGIN      
        1 taaagaatgg gtagcacccc gcaataaata aacgaggatt taccggagcc tgagggtcca
       61 atgacaccga tgaatctgtt tttggagagt tttaaaagca cctcatcact ctggccttcc
      121 cggccaaaga acaagtgact ctcatcaata ctgaatggtc gtaatccggg gaaaggattc
      181 gccagggttt tcttttttac atctacagtt tcactcatgg caattatctt ttattttcca
      241 aaaagttctt tatatcatca aggggcaatt ctttgccacc atcaattata tcaacatcat
      301 aaagggagaa actctcctta ttaatattaa ttcctgtacc ggtaagtatt cctctcccta
      361 aaataggttt cgctctgcca aatccagggg cctttagcaa gtcaagaagt ttcattctta
      421 cccactgtaa gtttacttta ccgtaataaa ttaacgctag atcaaattca accagatttt
      481 caatatgttt ttgtcttaat tcaagcaggt ctccctggaa ggaagggtgg atgacctcat
      541 atcctgattt ttcaataaat tttatcaggg gattcacttc tttttcatca atcaggtcat
      601 atacaaggta gaccatcttt gtacccttat tttcaatttt gggtctgcta actccaattt
      661 ggaacttctt ttcgccgctt acatcaatca attcctccct gataatattt ttgaaatctt
      721 caagaggagt ttgtaaaatt tccgcacctt ccaaagctga aatatcgcgt tttatgtttt
      781 caataaaagt tcgctgacgt tcgcttgtat ttttcaggtc aggggatatc caaattaaac
      841 gattcagttt gccgcttttt cttccgtttg cgcttgtttc gccggcgatc ttattttgca
      901 aatcaacaac agagcgctct gatccctttg ggatttcacc ataagaattt cctatcaaat
      961 ggatagacaa attacattca tcaagttctt ttctaacagt ttcttccaat tcacctgaat
     1021 tcaccggcaa tgtagtttca ggcaatactt tgtagccgta tcgctgcaat tccctgcgaa
     1081 taatatttct ttgaattgac agatcatgac tggtttctgc aagataaacc gactttcttt
     1141 cgaataatgg ctttacggtt gcggctgttg tttctttggc cttcagaatt gcttcatgaa
     1201 tatcataggc aagatcaacg agtttcatcc agtaacttct ttcagcttcc ggtccaaaga
     1261 agtcattaat ctggtgatcc tcacctgttt ctacatcaac cataaacaat tcataaccca
     1321 gcatgttgcg caatttggtt ggctggtcgt cacgatttac tctggctttt gttactttaa
     1381 aaatgattcc cttttctatc ttgaaaaatt cctctactgt atctctattc tttcccactt
     1441 ccaaaaacgc gggggtaata accggaatga ataatttgta ggccgacagg tcaccggaag
     1501 agatagagtt tttctctgtt ttcatttcaa tgccaacttt ttctcccaag acctggaaaa
     1561 gcatcatgtc cagaaagcgc ataaagttgg aaacccagcc ggtttcgccc tcggtaatgg
     1621 gctcgttatc aactgcagcg caaagaatca atattttgct atcgattgcc atgtaattct
     1681 ctataattta gcggtttctt gtttgataaa tttagaactg atattactaa tgaagctttg
     1741 agccaattca tgctgattgg ccatcacatc aaaaaagtcg ttcagtgtga ttttgaaaat
     1801 tattgtttca cctttaacgg tcagagatgt cgtactttta ctctcatcca ttgtgaaaat
     1861 atctccaaat acatcttttt cttttaaagt aataagtgga tcatcttcat ggtaaagagt
     1921 tacatttcct tttcccacaa tgaaaattgg tttgtcgtta tcttcgtcaa acacttccac
     1981 ctttgcgcca tcttttaatt tcagcgttct gaacctgtca acaatctgcg aaaggagtat
     2041 acctgagatc tgggaaaata cgggaatatt tttcaggaag aaaatcttgt cgatagttaa
     2101 ttggaagtcg tccttaaaac tttcaaagaa gaaggtggaa aataattttt catcgagatt
     2161 tctctttaca gtttcatcaa gtcgctgacc tactctttga taggttctct tgtctttttt
     2221 ataaattatc caggccgccg tttccatgag aaaataatcc ggattgaata attgggcaac
     2281 aattcctttt gaaaccttga aatcatcaat attttcagtg ctttttaaag cgagagccct
     2341 ggtccagcgg tttatttgat tgtagtccct gttaattata taattgatga cttcgtaatg
     2401 cgagtacttt tccctggggt agaaaatttg aagttggcga actttatcat taacagaaat
     2461 attatcaatc aacgggaaga gctttggctt taaatccgga tgcaggaaca aatccaggag
     2521 ttcaattcca agtccaatgc cgtctgcagt tccggtttca atgttatgtt taatcagaat
     2581 aacctgctta ggatcataca gaaggcacag gagcatatac atgtgatcat agttactggc
     2641 aatctcttct tccagtgcat caaccagatt cttattgttt tcatcttcgg gtaattcaga
     2701 aatagcggcc atgttccaga tggcttttcc tatttcagca tccaaaaggt cgcggattac
     2761 gggaaactgt tgttcgttgg cctggaaatg ataaaacctc agagcttgca atatttgctt
     2821 cacaattttc ctatcatgga aatcgatttt tttccacatt aaccggaagg cttcatcacc
     2881 accaatgcgg gtcatgatct gcacaatctt cagcatcact ttttgggttt gtccggagcg
     2941 atggaaggct gtttcgaggg tgaataagca gggggggcct gcttctacca aagcagcgac
     3001 ggccgcatgc gaataagttg aagaattaag cagttctatg agaaccggcc aggtttcagg
     3061 acgcttaact tttctggccg ttgcaatcgc ggccattctt actttaggat cctgatctct
     3121 taacaaatcc agaaggatga aaatattctc cgcgtcaata agtcttctta aaagctttgc
     3181 tgcaagcagc cgatcttcca tttttatgga agttcccaac gcgtgcagcc ttgatgaagg
     3241 gattgacaga acttcagtca tttctgccat tcctttggct ctgcttgcca gttccttgat
     3301 ttcagatttc tcagggtctt cgtactccag gtctaaactt tttacactat tagtcgcaaa
     3361 ttctttaatt gtctctgaat cactatccag gaatttcaat attgaagtct caaacagagc
     3421 cgggtcaagc cgttccatta atttcaaacc atagataacg cggtcttctt tttcactttc
     3481 aacttcgtgc tgaagcactg agttgataga ataattcgtt ttctcttcct ggacatctcc
     3541 tttggttttg ttcagagtta attggagagt ttctttatag ttgtgatgca ttcttatcgt
     3601 agtgaaatac cacactatca gaataggaat caaaccaatg atgatataga tgaactgcag
     3661 ttgaaaatta ttcatcagga taatgacacc acctgccatc actccggcaa atgcggtaac
     3721 caatccgtca attttggttt gcacgtcaaa tttcacggca ctgtcgatag gcaggaagta
     3781 cagtttaaag gaaggaccat caagtgcatc cttgagacta tcaataaaga gtttgcaacc
     3841 cgcgatgatc aggaagaagt aaataaacca ttgttcgtca gcggtatacc ctacggaaaa
     3901 accaatcagg acggcaagcc cggtcaaaat ggcagtaagt attggattaa taatcaaagc
     3961 aatcttcaaa ccatagttgg ctataatcca gtctgttacg aaagtttgaa agaggaatga
     4021 aaagattact acggtggctt cgaaataagc aataaagcta ccgaattctt tgtcaccggg
     4081 atactgtgca cccgctacgt ttacaaaagc ataatccata aacaaaactg caaccatcga
     4141 gatgatcacg aaaatcatca tcagtgagat ataccggtcc tttatcatct ttgaaatggc
     4201 aagtctttgt aatttgacct ttccttcgct ggacttaaca atcagttgat gtttgccgga
     4261 catcatgaaa aaggtgacca aaaaacccaa aaccgcggtg gcactcaaaa tcaaaagatc
     4321 ttcagttctt ctcaatgact ccaccaacaa tccaatacta aacaaggcga caatggaggc
     4381 caccagttga ccggaatcaa ttccgccaat aattcgtttt gcctgacgga gattgaacaa
     4441 tcgaccaaag gaaccccaga atatcaaaag tgaaataaag ttaaaaggga cagcaagagt
     4501 aaatacagta aagtaaatta cctgcctgtc ataagatcct tctgcagtgg tttctattcg
     4561 ataatacgaa ccggcaacaa gcaaaagcat aagaaaaatc cagccaattg ccattccccg
     4621 gaatgatacc cgggtttgca gtagtgaata caggaatgtg aacacaattg caatgccacc
     4681 accaaccaca acggcaatag gaagctgagt agcttcatca aattcacgca cgaaaagtgt
     4741 aactgaaaca acatcatagg aggcaagaaa gatgcctatg gaaaatccaa gtgcaagcag
     4801 gtatgaaacc tgactatact cggcgggctc tatattaaga gctctgagca ggaatgattt
     4861 catttaggtt tagtttgttg aaatcgtaat aaggcctcaa aatagcaact tttttattct
     4921 aaagaaagat aaaaatatac aatcttaaat tgattcaatc caatagaaaa tactcataat
     4981 caatagataa tgagttttca tgaagggttt agtattggtg gacaagacgt taaacgcgta
     5041 catctcaggg cgcgaccaca tttgattcaa agcaggagga ctccctcctt gactaaaaat
     5101 aagggcaatc caacaaaaat ttaccttttc atcctattca tccccgggga tgagcatcga
     5161 actatcgcca tagctcagga accggtaatt attttcgatg gcatgccggt agacttttct
     5221 ccaatcatta ccaataaatg ctgctaccag cgtaattagc gttgatttgg gaaggtgaaa
     5281 attagttacc aaaccatggc aaatctgaaa tttgtagcca ggatagataa atagttccgt
     5341 ttttatcgaa attatatctt gttgagattt tctcatgtgt tccaaaacat tttgcaagga
     5401 ctccggatgt gggacagggt tgatctggta aggagtttcc tttcttatca cattaacaaa
     5461 cccgcctgga attcccagct gaacaccaaa ccagtatatt gactccaata cccttagaga
     5521 agtcgttcct atcgcgataa cttttttttc ccgcatcaag cttttgacag tttctttttt
     5581 aataataatt tcttcctggt gaatcgggtg ttcctttatg atttcaactt caacaggttt
     5641 aaaggttcct ccacttacat gcaaggttaa atagttggtt tcgatatttt ttgcttcgag
     5701 tttcctgaaa atgtttttcg taaagtgcaa gccggcagtg ggggcggcaa cagcgcctgc
     5761 cacgcttgaa taaattgtct ggtaatccag ctgatcattt tccgtcggtt ctctttttag
     5821 atatggagga aggggtatgt ttccaattcc agataaaatt tcggcaaagg tttgatgtaa
     5881 gttccaggtg aactccacaa tttgcttttc ccggtttatc aatttgatgc tgagctttga
     5941 attttgagtt gaagaatcat taatgatcag ttcaagaggt tcacccttcc atttttttag
     6001 atttccaatc atgcattgcc agacacaatt attccgggaa ttcattattt gatttgtttc
     6061 ggcaggaaca accggttcca agagtaaaat ttcaactttt gcaccggatg gcttattaaa
     6121 aaacaacctt gccttgatta ctttggtatt attgaaaaat aatgccgaat tcgaaggtaa
     6181 caggttgggg aaatctttaa aaagcttgtc ctcaatcatt ccccggttat aaaatagtaa
     6241 acgggaagag tctcttggac ttgccgggtg attcgcgatc agactctcat tgaggtcata
     6301 agtgaattcg gaaattaaaa tgggtgaatc cttcaaagca aaaattttct tcaaatttaa
     6361 agaagaagtt gttagtgatt atattcacgt tccaaagaat tgaaatgagc ctttcagcaa
     6421 attacaggaa gagaaatctg aagtttgagt ttgtagcgag aacatcccgg ggatcgtttc
     6481 gtgaaaaaga cagctattac attattctca gagaaggtga gaccatcggg attggtgagg
     6541 tatcgccgct caaaggttta agtgtggatg atgtgcccga aattgagaat gaaattgcat
     6601 taacactaaa gaaaatggaa ggcttgaatt ccagagaaat atttcctgat atttcggaat
     6661 ttttgaagca ccatgttgac gatagatttc cttcaatccg gtttggaatt gaaacagctt
     6721 tgatggattt gaagaatggc ggctccctca ttatctatcc cggggctttt gaaaaatctg
     6781 agtcgggcat agagattaat ggattgattt ggatgggaga tttggaattt atgctcactc
     6841 aaataacaac aaaaatttat gatggcttcc gttgtattaa aattaagatt ggttctctgg
     6901 attttgaaag agagtgtgac attttgaaat atgtgcggaa taaatatttt cgtgacaaga
     6961 ttgaaataag agtggacgcg aatggtgcat ttaaacctga agtcgcactt gaaaaacttg
     7021 atattttgtt tgagtatgat ttgcactcta tcgagcagcc cataaaaccc ggtcaaaagg
     7081 atgtgatgaa atcattgtgc agacaaactc caattcctat tgttttggat gaggagctaa
     7141 tcgggattca tgcgcaaggt gaaatgcttt cattgctgga atttatcagg ccccaatata
     7201 tagtactaaa gccaaccttg ttaggtggat ttatcaggtg caatcagtgg attgaatgtg
     7261 ccgaaaagct tgaaataggg tggtgggtta cttctgcctt ggaatcaaac attggtttaa
     7321 atgcaatatg tcagtatact gataatcttt cagttaagat cccacagggg ctgggtaccg
     7381 gtgaattgtt taccaataat attccgagcc ctcttgagat aaaaaaatcg gaaatacgct
     7441 taaacaaata taagaattgg gatgttagtg aaattctgaa agaatagtat caaaaacttt
     7501 tctcattcca atggttgcct tctcttcaat taagaacaat ttatcctcgg gagcgatggt
     7561 tggcattttg ggatcaataa taaatcgggg gattcctctt gaaacataat caaggagacc
     7621 ggctgcggga taaacaagca gagaagtgcc gaccacgatg aagatatcgg atgcagacgc
     7681 ttcttttatg gccggctcca tggctggaac catctcacca aaccagacaa tattgggtct
     7741 gagttgactt cctttttcgc atttgtctcc aagtttgatt tcacctttga tgggatacac
     7801 cagggatgga ttatgtgttg accttgcctc gaaaatactt ccgtgcaagt gaataacttt
     7861 tttcgaccct gctttttcat gcaggttgtc aacgttttga gtaacaaccg ttacttcgaa
     7921 ataatcctga agtttagcaa ttaattcatg ggcaagattt ggattagcct ggagggctgc
     7981 cttccttctt tgattgtaaa attccagaac catttcgggg ttccgatgcc aaccttccgg
     8041 tgtagcgaca tcttctatat tgtgtccttc ccaaaggcct cctgaatcgc ggaatgtagg
     8101 aataccactt tctgcactta cccctgcacc ggtaagaaca actatttttt tcacttattt
     8161 cttccgtctg ataaacttct tcttttcagg agccttttcc accagttcca ggcattcttc
     8221 caaggttaac tcctcaggaa tcttgccttt tggaattttc acatttttac ttcctgcttt
     8281 aatataggga ccaaacctac cattcaaaac ctggatatcc ggattttcag gaaagctccg
     8341 gatcagtttt ttagaatcag cttcacgttt ttccttgatc aatccgatag cacgctccaa
     8401 ttcaactgag taaggatcgt cttccttcgg aagggagata aattttcctt catgcctcac
     8461 ataaggaccg aacctgccaa tattgacatc cactttactc ccttcaaaat cacccaattc
     8521 tctgggtagt ttaaataatt cgattgcgtc ctccagcgta atggattcta taaattggcc
     8581 attgcgcata cttgcaaacc ttggcttttc ttcttcttcc tcaccggtgc caatttggac
     8641 tacaggacca aattttccaa gccgggcaaa aattggttta cctgattttg gatctattcc
     8701 caactttctc attggaccaa cgtctgactt tttcagtgtg tttgtaaatt ctacgcgttc
     8761 atgaaaatta ccatagaagt ctttgagcat tttgttccac actttcttac cactggcgat
     8821 gtcatcaaac tctttctcta cctccgctgt gaatgaataa tttgttactg ttggaaaatt
     8881 ttcaacaaga aaatcattta ccaccattgc ggtgttggta ggaaataatt tatttttttc
     8941 gtagcctgta atttcagttt caactgactt agtaatttta ccatccctta acctgaaaga
     9001 ttgatatttt ctttccttac cgtccctggt ttcttttgaa atataacccc gcttctgaat
     9061 cgtactaatt gtaggagcat aggttgaagg ccggccaata ccaagttctt ccagcttttt
     9121 tacaagactc gcttctgtat atcgtggtgg atgcttggag aaaacctccc tggatttaat
     9181 ttcatacatg tttaaaacct ggttctcccg gaggggcgga agaatgccgg aggagactga
     9241 attttcatct tcttcttccc gtgattctac atatacctta aggaaaccat caaaaataac
     9301 tacttcaccg gtagcaataa atttttcggt tgtagtagaa atgccaattg ttgccgttgt
     9361 cttttctaac tgagcatctg ccatttgact tgcgattgcc cgtttccaaa ttaattcgta
     9421 aagcttctgc tcacgtttgt cattacttcc gatcaaatca gaaaaatcag taggcctgat
     9481 ggcttcatga gcttcctgtg caccttttga tttggtttta taatgtttta tgtgaaggta
     9541 tttatctccg taagactgct taatcctgga cgcggcatta ctgatcgcat cctcggaaag
     9601 tgataatgaa tcagttctca tgtaagatat ttttccagct tcatacaatt tttgagcaag
     9661 tatcatggtt tgagaaactg aataacccag tttgcggcct gcttcctgct ggagggttga
     9721 agtggtgaaa ggcggagacg aagatcgctt cccgggtttc ttttgaagtt tttcaatatt
     9781 gaattctgct cccaaacatt tattaaggaa atcttctgcc ttatctattg aattaaatct
     9841 tgatgaaagt tccgcattaa gttccgagcc atcatctaat ttgaagaggg cttgcaccct
     9901 gaaatttgac ccggacttaa aactttcaat ttccctttct ctttcgacaa ccaggcgaac
     9961 cgcaacagat tgaactcttc ctgccgataa acctgtttta atttttttcc aaagtatcgg
    10021 agataattca aaacctacaa gtcgatcgag tacccttctt gcttgctgag cattcactaa
    10081 atccaaatca attcccctgg gattctccag cgcattctga atcgcgtttt ttgtgatttc
    10141 atgaaacaca attcttcttg tcctggcatc ctctaaattg agcgcctctt ttaaatgcca
    10201 ggaaatagcc tcaccttccc ggtcatcatc actggcaagg taaatcaact cagcatcctt
    10261 gactaacttc ttcaggtttt caattacctc ttttttatct ttcgaaattt cgtacttagg
    10321 ttgaaaccca ttttcaatat caatggcgct atcatccttg ggtaaatcgc ggacgtgacc
    10381 atagctggaa gcaactttgt attctttgcc caaatagcct tcaatggttt ttgcttttgc
    10441 aggagactct actattacca ggttttttgc cataattcaa ttttcaaaaa cggctcaaat
    10501 atcaagaaaa aaatattaat aaaaagattc aaatattttt aaatgcggaa tataataagg
    10561 taagtaacca ttaactttcg gtttattttg aattcaatgg aggcagaatt attcctgatt
    10621 aggcatgcag aagcgttgga taagtcaccg gaaatcaaag atttagaccg tcaattatct
    10681 ccgattggat taagaaattc ttccattcaa ggagaattaa taaaaaaaaa taaaattctt
    10741 cctgatctga tagtatccag caatgcggta agagcagcat acactgctca tcttattgcg
    10801 gagcgaatag gatatgatac ttcaagaata ataataaatg acgaagtgta cgaagcctca
    10861 gtaagaacat tattaaatgt ggtaaaccag ttgaatgatg aattgaataa ggtattcata
    10921 atcgggcata atccttcaat ttcatatctg gctgagtaca ttagcaaatc ggagatagga
    10981 gatattcttc cgggctcaat tgtcagacta aagcgactta cggattcgtg gtcgttagtt
    11041 tctgaaaata cttgtgaatt acaatcatat aattatcccg gagaaactga tgaaataaat
    11101 aatgagtgaa attgaaaatt ttgatccgaa tggagtgggg gtaaagggaa ctttattcgg
    11161 gctgccgttt aatgaagaaa atgcttcact gattctaatt ccggttccat gggaggttac
    11221 ggtttcttac ggagcaggta ctgctgaggg tcctatgaat atacttgatt gctcaacgca
    11281 agttgatttg ttttctccac agattaagga tgcctggaaa atgggaatag caatgttgga
    11341 aattgatcct gtaattcagg aaacaagtta caaacttcgt gcagaagcgc tggaatatat
    11401 tcaggagcag gaaatgggtg atgatgattt gggagaaaaa cgaaattttt ctgacattcc
    11461 ttcccgggtt gatgagggat ctttacaaat gataaattgg gtgcgtgaaa agacggcaaa
    11521 cattttatcc aaagaaaaaa tggtaggatt gattggtgga gatcacagta cgcctttggg
    11581 tttaatagaa gcacttggtt ccaaatacag tgaatttgga attttgcaaa tcgatgcgca
    11641 tgcagacttg aggaaagcct atgaaggatt tacctattct cacgcgtcta ttatgtacaa
    11701 cgcgctccaa aatgaatcca taggtaaatt agttcaggtt ggagttcggg atttttccga
    11761 agacgaatac caaatgatca aggattcagg gggtagaatt tcctgcttct ttgattctga
    11821 cctgagagag cgccttggaa ataatgagag ttggaaaaca atttgcaaag aaataattag
    11881 tgaattgccc gaaagtgtgt atataagttt tgacatagac ggaatggatc ctaaattatg
    11941 tccgggcacc ggtacacctg ttcccggagg tttggaattt gacatggtaa caacattgat
    12001 tcgtgactta tcgctttcgg gaagaaaaat aattggattt gatcttgtgg aagtcggcgc
    12061 tcatccctgg gatggtaatg ttggtgcgcg attactgtac agtctttgca actggatggg
    12121 tgtttctcaa aaaaagttgt cacccgccac ttaatttttt ttctatcctt caacctatat
    12181 atttgaaact ttaaccataa accaaattgg aaattttcaa gctaatcgaa tcaggaattg
    12241 tcagcttcgc aaattggatc tgggattacc cgttactgat cttgctaatg ggaggcggct
    12301 ttttctttgt ggtttattca aggttccttc cattcaaata ttttgggcat gccctgggcg
    12361 ttttatcggg aaaatacgac aaggctgatg aagtaggcga gataaatcat tacgaagcac
    12421 taagtacaca ccttgcggct actgttggca tgggtaatat cagtggggtt gcggtcgcta
    12481 tcgccgttgg cggacccggg gctattttct ggatgtggat tagcgcactg gttggaattg
    12541 ccacaaaatt tttcacttgt tccctttcaa ttatgtaccg gggaaaagat tcattaggcg
    12601 caacgcaggg tggacccatg tacgtcattg ttgaagggct tggcaaaaga tggaagccat
    12661 tggcagtatt gttttgtctt gcaggaatga ttggagtgtt gcccattttt caggctaatc
    12721 aattgacaca agcaataagg gatgtggtcc ttgttcctaa tgggataggg caaggaggat
    12781 ttgtatcaga tctaataacc ggaattgttt taacaattct cgtatccgtc gtaattcttg
    12841 gtggtattaa aagaattgca acggttgcag gtaaactggt tccattgatg gtggttttat
    12901 atttcgttac tgtaatgatt attataatga tcgactacca tgatatcatc ccatcattcg
    12961 ccctgatttt tgaagacgct ttctccggaa atgcggtaat gggtggcgcc gtgggcgcgg
    13021 tcattttaac tggagccaag agggcggcat ttagcaatga ggcaggtatc ggaaccgcgc
    13081 cgatggctca tggagcggca aagacagatg aacctatcag agaaggattg gtggcaatgc
    13141 tgggacccgc aattgataca ttgattgttt gtaccctgac tgctcttgca atcataataa
    13201 ccggtgtgtg gaaaacaact gatcatgatg gtgttactct aaccatggct gccttcaatt
    13261 cagcattgcc tggctttggt ggatacatcc tgatcctgtg tgcgtttatt tttgcaacca
    13321 cctctttatt ttctttatct tattatggaa ctaaatgctg gtcatttctg gtgggtgctg
    13381 ataaaaccag gtactacgaa tatttctacg taatatcaat tattatcggt gcggttgcct
    13441 ctttaaccgc cattatcagt ttgatagatg ccttctatgc aattatggca attcctacta
    13501 tgacatcggc agtacttctt gcaccaaaag tattaaagga agcaaaaaga tacttttctt
    13561 cattaccaaa ataactattt tttacctgct aactcttcaa ggtatttgat gaaagatttt
    13621 ttgttataat ccgccattcc aattgttttg cttacaacca ttccatcagg actaataata
    13681 aatgttgtgg gaacggccgg aggattataa atctctggca aagggctcaa tgggaagaat
    13741 gcaggaagat caaagttttt cctatgtaga aattgaatcg cgtccccgga ttgggaatcc
    13801 aaagatatta atatgaattt cactttagtg gtgtccgttt tattataaag attctggatt
    13861 cccggcattt cggcaataca tggcccgcac caggatgccc agaagttcat aaaaataact
    13921 ttaccccggt agtcactcag gttgattatc ttttcagatg aaagagactt gagttccaca
    13981 tcatatactg ctctttccca tttatcagca ttgagtttct ttggagcaat tattccggta
    14041 gacaaaataa ttctttgggt aaaagcaatc acctcagtat gtagccctgt cgcgtatagg
    14101 attccgaata ctccaagaat tacaatccat tccctgacaa attttttcat tatcagaatt
    14161 aatatataaa cttaagtaca ctaaaagctt caaaaaagtt cacaaattgt tcggattagc
    14221 aaatctgatt gactgataaa aatagaaccg gtatgccaat tcatcgcaca cgaaatttga
    14281 tttgcccgaa aaatggtaaa ttgcaaataa aatcattcaa tgaaaagagc ggtagcttta
    14341 gtaattgtgg cactcatact tagcgtacaa aatgcttatt cacagaaagt taagtataag
    14401 gatttattta ttttgttgaa ttcaaagaag tatgaagaag ctgagccgtt tcttcgggtc
    14461 ttcatggctg atcccaaaaa ttctgaccat gccaacgcac atttccatat ggcattgatg
    14521 tttgaattta aagctaacag gaacgacata cttactcaaa atcacttact tgtaaataac
    14581 attgattcgg cgctttatta ctttaccctg gctaaaaaca tgatcgatga aaaggagact
    14641 agtaagaatg atgaatatta ccagacctat tcacgaagag atttgaggac gggcaaattt
    14701 gggataaaat tatctgatat tcatttagag attgaaaaca aggttaaggt aatgacaggg
    14761 caaaaaagca gtttaacaaa tttgttcaca cattatgtaa gaacagaaaa gtattatact
    14821 gacgctgata gtgttttcct tatccttcaa aataaatata aaaaccagaa taatttattt
    14881 ttgaaagcag ggaatactga tttagccaac ttaaaattgc tggcagctaa atttgattcc
    14941 gtcaggtata attttaatca atacaagtct tatctcgaga aattccctga atcaaaatat
    15001 aatcaaaaaa ttactatagt agatgtagga agacttgagg aagttgcgat tatgccgatc
    15061 gattggaagg atgaaacaat aaaggtgaga aattaccggg agtggtcagc ttctactgaa
    15121 acggtgatta ctgttgagct taaaaagttg aaagaacatc tgacgacata taataatcag
    15181 cttgataagt taactgaaaa gattgtaaag gattctgttt cagcaaagaa tgaacttact
    15241 gcgctcattg accagatgtt agctgaggat ttaaagaaat atgatcgcaa tcctatgcct
    15301 ctggctatat ttgactataa agttaaaaag attcaatacc tggatttatt gtttcaatat
    15361 gatttcgatg aactgtattt gaaagacaaa attattgttg cgaaggccag ggcaaaattg
    15421 ggggaggaaa ccctggcaat agttaaagca atagctgagt ttaacctccc ggaggaaaca
    15481 attaagtatc cggagttttt tcagacgata ggggagacag gtggaatttc aacacttttg
    15541 gcgaatggaa agcgcgaaat ggaagaagcg gtaaacaaat ggacactgga aatggaaata
    15601 ataatggaaa gaatgaattg gggtatcagt caggctgatt caatttcatt ggtgcctgca
    15661 attgcacaga gttacaagta tcacaccctt cttgtgaaag attctattgc cgaggcaaga
    15721 gccatagttt ccggtttggt gagctccgaa tctaaactga cctttacagc tataatagat
    15781 gaagattatg agatagacac attatttgct tttgaaatgg atccttttat taattcagtc
    15841 aggcatgaaa taattccatt aatgcaaata acggatctca ataatattct gactggattt
    15901 tatgcattga aatctgatac cgtgtctaga atggatttaa gtctaatctc cattaactcg
    15961 gtttcatgga ccactcattt agatgtgccg ggtaaaattt caaagattat ttatgtcccg
    16021 gatgtggagg aggttaaagt atttataaaa cgaaacgagg aagatgtgtt ggctttaacc
    16081 ctggattcag gaggcaagcc caaggatcaa tgaaagaata agtatcctac aaaaatcgcg
    16141 gcgataatgc ccgctaaatc cgcaataagt cctgctgttg ctgcatacct gattttcttg
    16201 atacttaccg agccaaagta caccgcaatt acataaaatg tggtttctgt ggccccgcga
    16261 aatacacttg ataattgtcc tacaaatgaa tcaggaccaa atgttttcat aacttcaatg
    16321 ttcatgcccc tggccgcact tccactaaga ggcttcatca atgcggttgg taaagcctga
    16381 acccagtcgc tattgaaacc caaacctgca acaacaaatg aaatacctga aacaatgtaa
    16441 tcgagtgctc ccgatgcccg gaatactcca atggctacca gtatggcaat taggaacggg
    16501 ataatcttaa tcgaaatttg aaatccttct ttggcaccgt caataaatgc ttcatacaca
    16561 ttaactttct tagcccaacc caaaacaata aagaagataa tcactgaaaa aataatgacg
    16621 ctgctcgcca cactagagac agaatttaat tgatcctgat ttaaggaagt gaagtaataa
    16681 acgagaccaa taattattat tgtaattcct ccaaggtaag atagaattac tctgtccagg
    16741 agattgattt tctgatagag agctactgtt attaatccga caattgtgct gaagaaagtt
    16801 gctattaaaa taggtataaa gatatcggtt gggttagaag cgccaaccac agaccgatca
    16861 attagtattg taagcggaat tatagataat cctgatgtat tcaaaaccag gaacattatc
    16921 tgagcatccg atgctgtatc cttgtctgga tttacttcct gcatttcttt cattgttttt
    16981 aagccaagcg gtgtagcagc attatcaagt cccagcatgt ttgctgagaa attcattatc
    17041 atacttccaa tggcaggatg gttacttgga attccgggaa ataacctgct aaaaaaaggt
    17101 ccaattgctt ttgataggag gcgaataatt ccaccatttt ccccaatttt cattattcct
    17161 aaccagaaag acataactcc ggttagatat aaacttagtt caaacccggt cttggccata
    17221 tcaaaggttg aattgaaaat tgcgggaaat acttctgtat cctgaaagaa aacaagctta
    17281 acaaggccaa caatgaaggc gatcagaaaa aaggcaatcc ataaataatt taaaaccatg
    17341 ctcagcaatt aattaaatta agggctgata ctgcgtagac accggctgtt tcagtcctta
    17401 gagtattagc actcaaagag actttttgaa agtcattttc ttctgcaagt cttatttcat
    17461 cctgtgtaaa atcaccttca ggtcctatta ctacacaata cgatttgtta ggtgttgcca
    17521 aattatacag gtggttgggt tgcaatttat caacataaca aataaatcgc tcatcctcac
    17581 tactcgaaga aattaattgg gtgaatggaa ggattgcttt aagctcaggg aggaatggat
    17641 tatgtgattg cttaacggca ctggcaattt tttttaataa tcgcgcttct cttattttta
    17701 ttctttcaga atgccggcag ttaataattg tgattttctt gactccaatt tctgtgcact
    17761 tttctataca ccattccagt ctttcatgat tcttggtcgc tgcaatagct aaatgaatcg
    17821 aataggacct ttgtggaata atttctttat gtaaaattct aaattgaact tttaccgggt
    17881 tggcatcaat tatttcaaca acagctttgg ctccttttcc gttaagaatt tcaatgttat
    17941 ccccaaccgt ctgtcgaagt accctgatac agtgattcga atcttcttca gtcagaaatt
    18001 caaccccatt aagaaactcc ggttgaaaga aggcaatcat gatttaagac aatttttctt
    18061 ttaattggct tactaaggca atgtattttt gcatcgcttc ttccgcgccc attcccttaa
    18121 gtttttccca ggtggaatat tttgcaattg ctttaaagtc gaagccggaa ggtttttcac
    18181 cggttatgtc gccatcagtt gcctgtttat agaagctgta aagttgaagc aaatcttcat
    18241 tggagggtct tgtagttaac gaatttactt ctaatgttgc tttttggaat tcttctttta
    18301 acatgacagt ttagttatta aaaaaggccc gaaggcccct ttcaattatc gtttggtgta
    18361 atcaatgtag tctctttctg tggctccaat gtagatttgc cttggtctgc caatgggttc
    18421 ggtattctca atcatttctt tccattg
//
LOCUS       DASWHX010000259         4944 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_3671500, whole genome shotgun sequence.
ACCESSION   DASWHX010000259 DASWHX010000000
VERSION     DASWHX010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 4944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4944
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3671500"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(179..2737)
                     /locus_tag="VGA21_15865"
     CDS             complement(179..2737)
                     /locus_tag="VGA21_15865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017732405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEX9652039.1"
                     /translation="MEAKFSNRVKEVISLSREEALRLGHDYIGTEHLLMGMIREGEGV
                     AISLLKKLGVSLDELRLAVEQATKGTASHNVKNLANIPLTRQSEKVLKITYLEAKIFK
                     SQLIGTEHLLLSILRDEDNIATQILEKFEVTYDVVKELLEYQTENPISSASETDDDDD
                     DSSRIFGGGSTGGRDPGAKGTEKSRTPVLDNFGRDLTKLAEVDKLDPIVGREKEIERV
                     AQILSRRKKNNPILIGEPGVGKTAIAEGLALRIIQKKVSRVLFGKRVVTLDLASLVAG
                     TKYRGQFEERMKAVMNELEKSPEVILFIDELHTIVGAGGASGSLDASNMFKPALSRGE
                     IQCIGATTLDEYRQYIEKDGALARRFQMVMVDATTPEETIQILENIKEKYEDHHHVNY
                     TKEAIKSCVNLSDRYISDRFLPDKAIDVMDEAGARVHINNIHVPENIVKLEGQIEEIK
                     KEKNKVVKSQKYEEAAQLRDKEKKLLEQLEIAKGEWEDETKTKRYTVTEDNVAEVIGM
                     MTGIPTKRVAQNESNKLLEMETELKGKVIGQEEAIRKLVKSIQRTRVGLKDPRKPIGT
                     FIFLGPTGVGKTELAKVLATYLFDKDDSLIRIDMSEYMEKFSVSRLVGAPPGYVGYEE
                     GGQLTEKVRRKPYSVVLLDEIEKAHPDVFNILLQVLDDGILTDGLGRRVDFRNTILIM
                     TSNIGVRDLKDFGAGIGFTTSTTTGMYSDEVMKSTIQSALKKAFSPEFLNRLDDVIVF
                     NSLQREHIHVIIDLTLNKLFARVKDLGYSVELTEKAKDFLSEKGYDPQYGARPLNRAI
                     QKFLEDPVAEEILKGEVGEGDIIVADYEKDKEELTIKHKKKRSGKEKKEKEDDG"
     gene            complement(2849..3475)
                     /locus_tag="VGA21_15870"
     CDS             complement(2849..3475)
                     /locus_tag="VGA21_15870"
                     /inference="COORDINATES: protein motif:HMM:NF020463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbqC family protein"
                     /protein_id="HEX9652040.1"
                     /translation="MHFKKVIIEPHYFPCLEYFQVLYYSEKVILEMCENFLKQNYNNR
                     CYILSANGPLALSIPLHKSSKLPYKEIKIDYSQDWISNHWRAITSAYGNSPYFNYYSE
                     LIRKLIEEKHTYLLDLNLSIMSNCLEMLGWQINLSCTLEYSKGIQEGITDLRSVIHPK
                     KDSILEFKFPSISYMQVFGREFVKNLSIVDLLFCEGPNAGYKLAGKKD"
     gene            3522..4577
                     /gene="mltG"
                     /locus_tag="VGA21_15875"
     CDS             3522..4577
                     /gene="mltG"
                     /locus_tag="VGA21_15875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009581278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEX9652041.1"
                     /translation="MKKRKILAAFIITFAVMLSSVSFYTYQIVNVPNILVEGEDMYLF
                     IPRNATFRSVQDSLFDKGIVKDPISFSFLAKLMNYDKQVKPGRYLLRREMNNLSAIRY
                     LRAGIQDPVDITFNNVRLLPELAKKICRNIEITPDEFLPALNDFVENNHEGFTDRNVM
                     AMFIPNTYEVYWSITATDLVGRMHAEYLNFWNEERIQKAREIELTPIEVSILASIVQA
                     EQLVHPDERPVIAGLYHNRLKINMKLDSDPTLIFATGDFSIKRVLNIHKEIDSPYNTY
                     KLGGLPPGPINMPDINSIDAVLNFSKHNYLFMVAREDFSGYHRFATNIRDHINNANRY
                     QRQLSIEQRKARMQNSN"
     gene            4577..>4944
                     /locus_tag="VGA21_15880"
     CDS             4577..>4944
                     /locus_tag="VGA21_15880"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009184542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEX9652042.1"
                     /translation="MYQYEVRQRIRYGETDQMGVVYYGNYALFYETARVEGFRNIGFS
                     YKKLEESGIMMPVLTMKSRFLKGATFDDPITVKVTVPKMPGVKITFLYDIYNEAGELI
                     HQGETELAFVKMKTGKPCKPP"
BASE COUNT         1415 a         1072 c          867 g         1590 t
ORIGIN      
        1 agaaatatct aaacttaaaa agatttgact gctaaactct attactcaaa acctaatcaa
       61 tttgaacagg tttttttatg cgtaattctg gtttttggaa ttcaattttc gtcatgaaat
      121 tggaacacaa aaaaggccgc tctatcgaac agcctatttg cgttatcggg aaaaatattt
      181 acccgtcgtc ttctttctct ttcttttcct tgcccgatct ttttttcttg tgctttatcg
      241 taagctcctc cttgtctttt tcgtaatctg ccactattat atctccttca cctacctccc
      301 ctttcaaaat ctcttcggca accggatctt ccaggaattt ctgaatggcc ctgttaaggg
      361 gtcttgctcc gtactgggga tcatatcctt tctctgacaa gaaatcttta gccttctcag
      421 taagctctac actgtaaccg agatctttta ctctcgcaaa taatttgttc agcgttaaat
      481 caataatcac atggatgtgc tcgcgctgca agctgttgaa aacaattaca tcatcaagcc
      541 tgttcagaaa ctccggacta aaagctttct ttaacgcgct ctgaattgtg gatttcatga
      601 cctcatcaga gtacatgcct gtagtagttg aggttgtaaa acctatcccg gcgccaaaat
      661 ctttcaagtc cctcactcca atattagaag tcattatcag aattgtattt ctgaaatcaa
      721 ctcttcttcc aagaccatct gtcaggattc catcatctaa tacttgcagc aaaatattga
      781 aaacatccgg atgcgccttt tcaatctcat ccagtaaaac tacgctataa ggttttcttc
      841 ttactttctc ggtaagctgg ccgccttctt cataacccac ataaccagga ggtgctccca
      901 ctaatcgcga tacactaaac ttctccatgt attcgctcat gtcaatgcgg atcaaactgt
      961 catctttatc aaacaggtaa gtcgccagta cttttgccaa ctcggtttta ccaacacctg
     1021 taggaccaag aaagatgaat gttccaatag gttttctagg atcttttaga ccaaccctgg
     1081 tacgttgaat agattttaca agcttcctga tagcttcttc ctgcccaata acttttcctt
     1141 tcagctcagt ctccatttca agaagcttat tactttcatt ctgagccacc cgcttcgtag
     1201 gaatacccgt catcatacca attacctcag ctacgttgtc ttccgtaacg gtataacgtt
     1261 ttgttttagt ttcatcctcc cattctcctt tagcaatttc cagttgttcg agtaatttct
     1321 tttccttgtc cctgagttgt gcagcctctt catacttctg actttttaca actttattct
     1381 tctccttctt gatttcctca atttgtcctt caagtttcac aatattttca ggaacatgaa
     1441 tattattaat gtgcactctt gctccggctt catccattac atcaatggcc ttgtcaggta
     1501 aaaatcgatc actgatatat cgatctgata aattgacgca agactttatt gcttccttag
     1561 tataattcac atgatgatga tcttcatact tttctttgat gttttccaga atctgaattg
     1621 tttcctcagg ggtggttgca tctaccatca ccatttggaa tctccgtgcc aaagcgccat
     1681 ccttctctat gtactggcgg tactcatcta aagtggttgc accgatacat tgaatttcac
     1741 cacgcgacag ggccggttta aacatattac ttgcatccag ggaacctgaa gcaccacctg
     1801 cacccacaat agtatggagt tcatcaatga acaaaatcac ttccggggat ttttccagtt
     1861 cgttcattac agctttcatc cgctcttcaa actgacccct atactttgtt cccgctacca
     1921 aagaggcaag atcgagagta actactctct taccgaacaa aacacgggac accttcttct
     1981 gaatgatgcg aagcgcaaga ccttctgcta tcgcagtttt acccactccg ggttcaccaa
     2041 taagtatcgg attatttttc tttcttctac ttaatatctg ggcaactctt tctatttctt
     2101 tttctcttcc gactataggg tcaagtttat caacttctgc aagtttggtt aaatctcttc
     2161 caaaattatc cagcacagga gttcgtgatt tctcagttcc tttagctccg ggatctcttc
     2221 caccggtact tcccccacca aaaatcctgc tgctgtcgtc atcatcatcg tcagtttcgc
     2281 tggccgaaga aattggattc tctgtctggt attccaggag ttccttaaca acatcgtagg
     2341 ttacttcaaa cttttccaga atctgtgtgg caatattatc ttcatccctt aaaattgaaa
     2401 gcaagagatg ttctgtacct attaattggc ttttgaaaat cttcgcttca aggtaggtaa
     2461 tctttaatac cttttcagat tgtcttgtta agggaatgtt agcaagattt tttacattat
     2521 gtgaagcagt tcccttggtt gcctgctcta cagccagacg caattcatcc aaagaaacgc
     2581 caagtttttt aagaagactg atagccactc cttcaccctc tctaatcatg cccatcagga
     2641 gatgctcggt tcctatataa tcatgcccaa gtcgcaaagc ttcttcccga cttagggaga
     2701 ttacttcttt aactctgttt gaaaatttag cctccataga gattaataaa tcatgttact
     2761 agtaaatcta taatatttag gactaaaaaa catcaacgtg taatttattt ataaataaca
     2821 ataaattggc gttaaggttc cttcactctt aatctttctt acctgccagt ttgtaacctg
     2881 cattgggtcc ttcgcagaaa agtaagtcaa ctatgcttaa atttttaaca aactctctgc
     2941 caaaaacctg catataggaa atagaaggga atttgaactc taaaatagaa tctttcttgg
     3001 gatgaatcac tgaccttaag tcagttatgc cttcctgaat gccttttgaa tattccaagg
     3061 tgcaagacag gttgatttgc cacccaagca tttcaagaca atttgacatg attgataaat
     3121 taaggtccag gagataggta tgtttttcct ctataagctt cctaatcaat tcggagtagt
     3181 aattaaaata gggtgaattg ccataggctg atgtgatcgc tcgccagtgg ttagagatcc
     3241 agtcttgtga atagtcaatt ttgatttctt tgtaaggtaa tttggaggat ttatgcaaag
     3301 ggatggagag ggctaatggc ccgtttgcag ataagatata acagcgatta ttataattct
     3361 gtttcaggaa attctcacac atttccagga taactttttc tgaataatac agaacctgaa
     3421 aatattccag gcagggaaaa taatgaggct caataatcac ttttttgaaa tgcataaggt
     3481 tcaatatgta ttcattttca acaatgtgtt acttttgcat tatgaagaaa agaaaaattc
     3541 tggcagcatt cataataacc tttgcagtaa tgctctccag cgtttctttt tatacatatc
     3601 aaattgtgaa tgtaccaaac attttggtag agggagaaga catgtattta tttataccaa
     3661 gaaatgcaac ttttcgctct gtacaggatt cacttttcga taagggaata gtaaaagatc
     3721 cgatttcatt ctcattcctt gccaaactga tgaactatga caagcaggtt aaaccgggac
     3781 ggtacctgct caggagagaa atgaataacc tttcagccat acgatatctg cgtgccggaa
     3841 tccaggaccc tgttgatatt acctttaaca atgtgaggct gcttccagag cttgctaaaa
     3901 agatttgcag gaatattgaa attactccgg atgaattttt acctgcactc aatgactttg
     3961 ttgagaataa tcatgagggc tttacagaca gaaatgtgat ggctatgttc attccaaaca
     4021 cctatgaagt ttattggagc ataaccgcca ctgatttagt tggtagaatg catgccgaat
     4081 atcttaattt ttggaatgaa gaaagaattc agaaagcaag agaaatagaa ttgacaccaa
     4141 ttgaagtttc cattcttgct tcaattgttc aggcagaaca gttggttcat cccgatgagc
     4201 gacctgtaat tgccggttta tatcataaca ggctgaaaat aaatatgaag ctggatagtg
     4261 atcctacgct tatctttgca accggagact ttagcattaa gcgagtgttg aacattcata
     4321 aagaaattga ttcgccctat aatacgtata agcttggcgg actgccgcct ggaccaatta
     4381 atatgcccga catcaattcc attgatgcgg ttctgaattt ttcgaaacat aactatcttt
     4441 tcatggttgc acgggaagat ttttccggat accaccggtt tgctacaaat attcgtgacc
     4501 acattaataa cgctaaccgg tatcagcgtc aactttctat tgagcagaga aaagcgagaa
     4561 tgcaaaattc aaactgatgt atcaatatga agtaagacaa agaattcgct acggggagac
     4621 tgaccaaatg ggagttgttt attatggcaa ctatgccctg ttttatgaaa ccgcgcgtgt
     4681 tgaaggtttc cgcaacatag gtttctctta taaaaaactc gaagaaagcg gaatcatgat
     4741 gcccgtactg accatgaaga gccgtttttt gaaaggtgcc acctttgatg atcctatcac
     4801 ggtaaaggta actgtaccta aaatgcctgg agttaaaatt acttttttat acgatattta
     4861 taatgaggcc ggcgaattaa tccaccaggg tgaaactgaa ctggcctttg taaaaatgaa
     4921 aacgggtaaa ccctgcaagc cacc
//
LOCUS       DASWHX010000260         3014 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_369097, whole genome shotgun sequence.
ACCESSION   DASWHX010000260 DASWHX010000000
VERSION     DASWHX010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 3014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3014
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_369097"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            108..>3014
                     /locus_tag="VGA21_15885"
     CDS             108..>3014
                     /locus_tag="VGA21_15885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020532468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX9652043.1"
                     /translation="MKKSLQWILIPLFSFPFYGQTQDKKWDVSNTGEPLKEIEIETSE
                     GTWMNLDVSPDGREIVFDLLGDIYIMPVGGGNATILREGPAYEVQPRFSPNGKQISFT
                     SDAGGGDNIWIMEKNGAEAKQVTKEDFRLMNNAVWTQDGKYLIARKHFTSQRSLGAGE
                     LWMYHITGGSGIQLTKRKNDQQDLGEPSVSPDGRYVYYSEDVYPGGFFQYNKNPNSQI
                     YAIKRLSLEDHKIETITGGPGGALRPQISPDGNWLAFVKRVRTKSVLYIRNLATGSER
                     PVYDELSKDQQEAWAIFGPYSNYNWLPDNQNIIIWAKGKIKKVNITNYQVIEIPFAAK
                     AKHKIINSVEFKNEVDPDQFTAKAIRHAVTSPDGKTLIFNAAGYLYRKSLPGGTPARL
                     TKGTDLEFEPAFSRDGRKLTFVTWSDVEMGAINVLDLTNSRATPTKITKEKGIYRNPS
                     FSPDASQIVYEKENGNSHQGFVYTEEPGIYLISASGGKPAKVKDNGVNPIFNRSGDRI
                     FFQSGGYLFGNLTKTYRSAKLDGTDEQTLFESKYAHRIIPSPDNEWVAFSQLYKVYVA
                     PFPKTGTTLNLDGKSNPFPVSQIARDAGINIHWSADSKKIHWTLGEEYFSIDINDRFT
                     FLEGSPDSIPPLDTAGIKINLILKSDKPGGQIAIKGATILTMKGDEVISNGVILIEGN
                     KITAIGTEDQVPYPNNAYVIDATGKYVMPGLIDAHAHVGQFRYGLSPNKHWEYYTNLA
                     YGVTTTHDPSANTEMVFSQSEMVKSGVMVGPRIFSTGTILYGAEGDFKAVINNLDDAR
                     SAMRRMKAFGAFSVKSYNQPRREQRQQVIKAAREYSIMVYPEGGSTFTTNMTMILDGH
                     TGIEHNIPVAPLYEDVIKLWAASGTGYTPTLIVNYGGLSGEYFFYQTTNVWENEKLLK
                     YFPRGSIDSRSRHRTMVPIEEYENGHILTSQSCKKLTDAGVKVNLGAHGQLQGLG"
BASE COUNT          956 a          578 c          686 g          794 t
ORIGIN      
        1 gccggatttc acaagatgct tgatgaactg gtaaagctgc aggagagctt tctcgaaaat
       61 aactgatcct ttagtataat tcatgctcac aacttaaatc tacctttatg aagaaatcat
      121 tacaatggat tttgattcca cttttctcgt tcccattcta tggacaaact caagataaaa
      181 aatgggatgt aagcaatacc ggtgagcctt tgaaagagat tgagattgaa acttctgaag
      241 gaacgtggat gaatctcgat gtaagtccgg atggccgtga aattgtgttt gacttattag
      301 gtgatatcta cataatgccg gttggcggtg gcaatgcaac aatacttaga gaaggtcctg
      361 cttatgaagt tcagcccagg ttttctccga atggtaagca aatcagtttc accagcgatg
      421 ccgggggcgg tgataacatt tggattatgg agaaaaacgg tgctgaagcg aaacaagtta
      481 ccaaagaaga cttccggtta atgaacaacg cagtatggac gcaggatgga aaatatctaa
      541 tcgcgcggaa acatttcaca agccagcgtt cgctgggagc aggagaatta tggatgtacc
      601 atatcaccgg tggctccgga atacaattga caaaaagaaa aaatgatcag caggatttgg
      661 gcgaaccttc tgtgagtccg gatggaaggt atgtttacta tagtgaagat gtttatcctg
      721 gcggcttttt tcaatacaat aaaaatccga actcccagat atatgcaatt aaaaggttaa
      781 gtcttgaaga ccataaaatc gaaacaataa cgggtggtcc tggtggcgcg cttcgacctc
      841 agatttctcc ggatggaaat tggcttgcct ttgttaaaag agttcgtact aaatctgttt
      901 tgtatatcag aaacctggct accggatcgg agcgccctgt atatgatgag ttaagcaaag
      961 atcagcagga agcctgggct atattcggtc cctactctaa ttataattgg ctcccggata
     1021 accaaaatat tatcatctgg gcaaaaggta aaattaagaa agtaaatatc accaattacc
     1081 aggtaattga aattcccttt gccgctaaag caaagcacaa aataataaac tctgttgaat
     1141 tcaagaatga agttgatcca gatcaattca ctgccaaagc catacgtcat gcggtaacaa
     1201 gtccggatgg aaaaacacta atttttaatg ctgccggtta cctttacagg aaatcccttc
     1261 ctggtggaac ccctgccaga ctaactaaag gaactgatct ggaatttgaa cctgcttttt
     1321 cccgggatgg ccgaaagtta acttttgtta cctggagtga tgtggaaatg ggagccatta
     1381 atgttcttga tttaaccaat tccagggcaa ccccaacaaa gattaccaag gaaaaaggta
     1441 tttacagaaa tccttccttt tcgccagatg caagtcaaat tgtgtatgaa aaagagaatg
     1501 gtaatagtca tcagggattt gtttatacag aggagccggg tatttacctg atttcagcgt
     1561 ccggtgggaa accggccaag gtgaaggata acggtgttaa tcctattttt aatcgatcag
     1621 gcgatcgaat cttttttcag tccggaggat atctctttgg aaatctgaca aaaacgtatc
     1681 gaagtgctaa gctggatggc acagatgagc aaacgctttt tgaatcaaag tatgcacata
     1741 gaataatccc aagcccggat aatgaatggg ttgctttttc acaattatat aaagtttatg
     1801 ttgctccctt tccaaaaacc ggaacaacat tgaacctgga cggcaagtcg aaccccttcc
     1861 ctgtttcaca aattgcccgg gatgcaggaa taaatattca ctggtccgct gacagcaaga
     1921 aaatccattg gactttaggg gaagagtatt tttctattga tataaacgac cgattcacat
     1981 ttctggaagg ttcaccggac tcaatcccac cacttgatac tgccggaatc aaaattaatt
     2041 taatcttaaa atcagataaa cctggtggcc agattgcaat aaaaggcgcc acaatactta
     2101 cgatgaaggg agatgaagtg atttcaaatg gtgtgatttt gattgaagga aataaaataa
     2161 ccgcaatagg caccgaagat caggtacctt atccaaacaa tgcctatgtg attgatgcca
     2221 cgggaaaata tgtaatgccg ggattaattg acgcacatgc tcacgttggc cagttccgtt
     2281 atggtttaag tcctaataag cattgggagt attacaccaa cctggcttat ggcgtaacaa
     2341 ctactcatga tccctcggca aataccgaaa tggttttttc acaatctgaa atggtaaagt
     2401 caggtgtgat ggtgggaccc agaatatttt ctacaggaac tattctttat ggagctgaag
     2461 gagatttcaa ggcggtaatt aacaacctgg atgatgcccg cagcgcaatg agaagaatga
     2521 aagcctttgg cgcattttcg gtgaaaagct ataatcaacc ccggagagaa caaaggcaac
     2581 aggtgattaa ggcggcgcgc gaatacagca taatggttta cccggaaggg ggctcaactt
     2641 ttaccactaa tatgaccatg atcctggatg gacacaccgg tattgaacat aatattccgg
     2701 tggcaccact ttatgaggat gtgataaaac tttgggctgc cagtggaaca ggctacactc
     2761 ctacactgat tgtgaactac ggtggattaa gcggcgagta ttttttctat caaactacga
     2821 atgtttggga aaatgaaaag ttgttaaaat attttccgcg gggttctata gacagcagat
     2881 caaggcaccg aacaatggtt cccattgaag aatatgagaa tggacacatt ttgaccagtc
     2941 aatcgtgcaa aaaactaacc gatgccggag ttaaggtaaa tttgggagct catgggcaat
     3001 tgcaggggtt agga
//
LOCUS       DASWHX010000261        23450 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Cyclobacteriaceae bacterium isolate SMAG_U2853
            k141_3684324, whole genome shotgun sequence.
ACCESSION   DASWHX010000261 DASWHX010000000
VERSION     DASWHX010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960500
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Cyclobacteriaceae bacterium (soil metagenome)
  ORGANISM  Cyclobacteriaceae bacterium
            Bacteria; Bacteroidota; Cytophagia; Cytophagales;
            Cyclobacteriaceae.
REFERENCE   1  (bases 1 to 23450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:35:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,197
            CDSs (total)                      :: 3,159
            Genes (coding)                    :: 3,153
            CDSs (with protein)               :: 3,153
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23450
                     /organism="Cyclobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3684324"
                     /isolate="SMAG_U2853"
                     /isolation_source="soil"
                     /db_xref="taxon:2099675"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..879)
                     /locus_tag="VGA21_15890"
     CDS             complement(<1..879)
                     /locus_tag="VGA21_15890"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009577757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HEX9652044.1"
                     /translation="MALVEMLMPKMGESIMEATVLTWLKKEGDKIDQDESVLEVATDK
                     VDTEVPATHAGILKEILAKEGDVVPVGKPIAIIVTEGDDTVTTKAPAREEKKEVKQES
                     RPEPVAAPVETISNNGSNGQKSTRFYSPLVKSIAKQEQIPLIELEAVPGSGKKGRLTK
                     YDILAYVEQRKSGGVLMPAYQETSSFSVISSGQDEIIEMDRMRKMIAERMVESKRTSP
                     HVTSFVEADVSNIVAWRNKYKNEFKQKEGDNLTFTPIMIDAVIRAIKDYPMINIQVDG
                     TKIIRKKDINIGMAVAL"
     gene            complement(926..2179)
                     /locus_tag="VGA21_15895"
     CDS             complement(926..2179)
                     /locus_tag="VGA21_15895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013452681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEX9652045.1"
                     /translation="MNNVYAEIITIGDEILYGQISDTNSQWISSELDLIGIKTRWRTT
                     VGDNEEDILNVFKEAKSRANLILITGGLGPTSDDLTRPCLAKFFNCELVLNDDQLKHI
                     TELFKKFGRELSETNKHQAELPEKCKPLTNPKGSAPGMWFEEDGKIFVSMPGVPREMK
                     HLMRGQVIPGIKEYFPTPFIKHKIIKTIGIGESYLSDKIKEWENNLPLELKLAYLPGL
                     GDVKLRLTCIGARESEIDELISIEIEKLKQLIGVHIYGYDKDTLAGAVGNSLRSKKLK
                     VACAESCTGGNLSKTITSVAGSSDYFQGCVIPYHNQFKSAILNVNKETLFTEGAVSEN
                     TVIQLADNVKSLFEADIGISTSGVAGPGGGTEDKPVGTVWIGLAGPWGTHAKRFSFGG
                     DREANIHFSTLAALFMLWQRLLEID"
     gene            2257..2982
                     /locus_tag="VGA21_15900"
     CDS             2257..2982
                     /locus_tag="VGA21_15900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009193505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4197 domain-containing protein"
                     /protein_id="HEX9652046.1"
                     /translation="MKTFKILIIATGLCISCPASAYQVKGLFKKAEKALTNSGLSEEE
                     IGKGLREALIKGISVGSDLASQLDGYFGNPSIKLPFPPDAQRVETTLRNMGFGAKVDE
                     FILTLNRGAEKAAKESKDIFIEAIRAMTIQDALGILKGEDDAATQYLMRTTSQTLFRK
                     FQPIIKRALDEVNATRYYADLVNTYNKVPLVRKANPDLDEYATNKAIEGLFYLIAIEE
                     GKIRKDPLARTSDILKKVFSQQD"
     gene            complement(2979..7094)
                     /locus_tag="VGA21_15905"
     CDS             complement(2979..7094)
                     /locus_tag="VGA21_15905"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX9652047.1"
                     /translation="MKSSLTFFLLVSLTHLQFTQSNNSTEESFILKIKEGKIQAVAQW
                     LKSRGFANEMEPVFPGRKPGSRLRNYHRIKAKFSTGEIIKILKSNPDIEILEKEKTYR
                     PLFVPDDPAAQPGSEQWHLAAMSAYLGWDLISPAPNPVITAIIDTGFDLSHEDLANTF
                     YTNDNDPVDGIDNDNDGYIDNFIGWDFGDNDNDPSSLSQAHGTIVAGAGFAAANNTLG
                     GAGTGYNSKYMPIKIHSTALNELTGAYEAIVYAVEQGAKVINLSWGDAGEYSQFEQDI
                     INYAAIDFDAVIIAAAGNTNEHLAFYPASYDNVLSVGSTNSSDQKTDWGTISEYIDLS
                     APGIDIYTTQPTNGYANSWGSSLAAPLVSGLAALVRQSFPLLNALQIMEKIRVSADDI
                     YSVPGNENFFGLLGKGRVNMRKALESSAGPVVRAFIQPDFIQPFYDDSLHFEVVFKNF
                     LDPISSPLTIALNTLSSNITMVSQSITLNSLNTLETSISYSFSVKIDELTPPSEFLQL
                     YFSFESGAYVDFQMLPFRNGPDFIEFNNEKITWTANSTGHLGYFDDYYNGHSLRFQNI
                     DYGWHAGIIAATDASHVSGTVINDFVTYTQDDDFETIRFVKSHQTPYSDIDYRLRIEE
                     KPSATAIGIRIDEKLLGWKNAEDQDFMILEYRVTNLNPESINGFDFGMFLDIDLEDYT
                     QNKTSFSSDSKLGYIYSDNEAFGIALLTNQNPVSRGIDFGNFNGNVPDIGITFGDTEK
                     FQFMNEGLTQTDAGVNGSGNDVAIVFGAVIPVIAPLESVTIAFSIIFGTSLTDLEAKV
                     SLAETRYDAYSQYHPELETFLACEGESAVINPANLNDFKLFSDAQHTDLLFTGNEFIT
                     PALQNQQTYYITSLINELEEESYSIQVDLHYANVDFIMSSASLVLHPGEKSSIAVQDI
                     TTGSISRTWDFGNGYGSINNDAKPEYSLPGTYNIELTSTDNLGCEESITKHLSVIESY
                     EKPVFPEFIIICYGENLSFSDSGKILNLYVDSISPPVASGSEIFTDDLISDTTFYLTQ
                     VGLKPESLPTRVDVIIENLMTGFDFSIDTTHFESPTVLQLNSETPGVSSEWYLGDTYL
                     GSGDTQIVNYSGLNELLIKQRVASEFGCLDSLVKSIPLKISPAPKEEMITICQNSFAS
                     LIPEPGSIFVFYNNKNEIIQKGRYLELTNLDRDSIFYFTSIDSLLESAPAKFEIKISF
                     PNSGFTPLKDTLMSNGISQIEFQAEEKLLSSYSWDFSSAGFSSVINPTVSFLLPGLYP
                     VQLTVTDSLGCNSTTLGSVLVADITSFDESDGTLIYFPNPVRNNQFFISGLTPGKVEI
                     LDLTGKGIEFQITKMPEGVMILLNENTSNGIYLVNVISSQHQKSFRIQVEN"
     gene            7430..8713
                     /locus_tag="VGA21_15910"
     CDS             7430..8713
                     /locus_tag="VGA21_15910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009582712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX9652048.1"
                     /translation="MNKRILIGAGLIISVVVLVLIYQSTKGSSSEDVEVLTEAQFGDF
                     IIDVTTTGELEAKSSVRITGPNGLQQAQIWQIKIDDIVEEGTLVKKGDYIAKLEQTQL
                     MDKIQQEQNDLSQSDSKYTEMKLDTALELRKARDELINLEYEITTKKIIVEQSKFEPP
                     ATQTAAQIEFEKAERAHKQAFDGYKLRKEKAVAQMQQAWAERQEDQTRVDFLMRLAQE
                     FTVTAPEDGMVIYARTWNGTKKGVGSTVQAWDPVVATLPDLNKMISKTYVNEVDIRVV
                     KVGQTVQVGLDAFPTKKLTGKVITVANVGEQKPNSDAKVFQVDILINESDTTLRPAMT
                     TSNTIIAEVVPGVVFVPLESLHSQGDTLTYVIKKDGLTFSKQEVKVGKTNADKAVILE
                     GVKQGEIVYLSNPTGYESKRLIKLPEKGTSVASSN"
     gene            8715..10043
                     /locus_tag="VGA21_15915"
     CDS             8715..10043
                     /locus_tag="VGA21_15915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020531489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9652049.1"
                     /translation="MTKYLFNFHIAVESVLSNKFRSLLTALGIIFGVAAVIAMMAIGS
                     GAQKEILEQIKLVGVNNIVITPVVEQKEEVLSDETAAKEKPNFSPGLTLYDARSLEKI
                     IPGITHLSPEIVLETNIIKNGRRRSAKLVGVDNSYFSVANFTFQGGYIFSKEQLEMGE
                     AVCIIGKGVQAKFFSQENPLGKMLKVGKHWLRVIGVLDQRFISEKSLTNLGIRDYNMD
                     VYAPLNTVLLRYKNRSLITERLLLRAQFQEEENEENKKAENYHQIDRLVIQVEKTEKL
                     TGVAEVASKLLKRRHYEMVDFEITIPELLLKQQQRTKSIFNFVLGAIAGISLLVGGIG
                     IMNIMLASVMERIKEIGLRMSIGAKKRDIIAQFLFEAVMISVSGGFVGVFLGVVFAIL
                     VSNFADIETSVTFISILISFGVAASIGIIFGITPAQRAAQQDPIVSLRHE"
     gene            10143..11804
                     /gene="paaN"
                     /locus_tag="VGA21_15920"
     CDS             10143..11804
                     /gene="paaN"
                     /locus_tag="VGA21_15920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017731764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation protein PaaN"
                     /protein_id="HEX9652050.1"
                     /translation="MDLFEKHKSIIEKAISALHKREFFAQYPEHPGAYPEDADAKGRE
                     KYMSLSGNYFDEISAEGSDGWIGVEVSPYTQEEINIKYPAFSVDTLISNANKSFGAWR
                     KASPETRAGILVESLEMLKERFFEIAYATMHTTGQGYMMAFQASGPHAADRALEAIAA
                     GYEELSRFPRQATWDKPMGKFNIKLEKEWKAVPKGIGVVIGCSTFPTWNSVTGLYADL
                     VTGNPVIVKPHPGAVLPIAIVVAEIQKVLRENGFDANICQLAVDTFEKTITKELAEHP
                     QVKLIDYTGNSEFGSYLEGLSGKCVFTEKTGVNSVIIDSVENADAVIQNLIFSLALYS
                     GQMCTAPQNFFIPEGGINTPDGIITFDEFAEKFVAATSGLADNPKAGPFVLGAIQNPK
                     TSERVKTAEKIDGKVWLKSKTIENPQFKNARTATPAIIEVNASNKDSFSKELFGPIAL
                     LIKTKNTNESVQLAKDIAMKFGAISCGAYTTNPEIKEMIKDEMSQVATPVSFNLVGGI
                     YLNQHAGFSDFHVTGGNPAGNASFTNPEYVIKRFVWVGFREPAKA"
     gene            complement(11807..12556)
                     /locus_tag="VGA21_15925"
     CDS             complement(11807..12556)
                     /locus_tag="VGA21_15925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009577769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX9652051.1"
                     /translation="MEIYQAIFLILTGILAGFLNTVAGGGSLLTLPLMIFMGLDSVVA
                     NATNRVAIIFQNIFAVAGFKSKGVFIYPYGIYLGLASITGAVIGAKISVEIDSDLFNK
                     ILAIIMVAVVVWTILDRSGNSDETKERLDKRHQIYGVIMFFFIGIYGGFIQAGVGFLI
                     IAGVTIVNRLSLVKTNSLKVLAALMYTSSALIVYIIEDKINWTWGLTLAVGNSAGGWI
                     ASRWSVKKGDKWIKRVLFVTVIVLAIRLWFF"
     gene            complement(12566..12790)
                     /locus_tag="VGA21_15930"
     CDS             complement(12566..12790)
                     /locus_tag="VGA21_15930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9652052.1"
                     /translation="MRKQFTVLGFYISFIVFALSLLFAYYGFTEADEDQKKGIVGLIL
                     FSSLFLFINWLNYRRFNQERGKQLKKSRRK"
     gene            complement(12804..13739)
                     /locus_tag="VGA21_15935"
     CDS             complement(12804..13739)
                     /locus_tag="VGA21_15935"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEX9652053.1"
                     /translation="MKIRAARTRIKNYIVKTELEFSEYLSSQCGCKVYLKLENRQVTN
                     SFKIRGACNKILTTKLSTKNGFVAASSGNHAAAFAYLAQQMGFVGIIFLPVTVPKAKL
                     DALKSYPVQVVQFGYDTLEAEYEAKRFGREKEMPYVSPYNNLSVIQGQGTLGLEIHDQ
                     LLNVHTVFIPVGGGGLAAGVGSYFKKARPLTQIIGCQPFNSKVMYESVMAGKILKLKS
                     ESTLADGTAGGIEKRAITFPICSRVIDDWVLVKEWEILDAIRIMISYHDMLIEGAAAL
                     TVAGLLKRHQDLEDKNVVLIITGERVTKDVLKELA"
     gene            complement(13742..14248)
                     /locus_tag="VGA21_15940"
     CDS             complement(13742..14248)
                     /locus_tag="VGA21_15940"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017731766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEX9652054.1"
                     /translation="MIVSLIAAVSENKVIGKDNKLVWDLPRDMKFFMDTTLGHYIITG
                     RKNYESIPEKFRPLKNRTNIIVTRQKKFLAKGAIVVHSLEEAFHLAAEAHQQEVFVIG
                     GGEIYRQSMNRADRLYITEVKAKFSGDTYFPDYYKAEWKEISRIQNLPDEKHAYPYNF
                     VLLEKIVA"
     gene            complement(14245..15168)
                     /gene="fmt"
                     /locus_tag="VGA21_15945"
     CDS             complement(14245..15168)
                     /gene="fmt"
                     /locus_tag="VGA21_15945"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018473228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEX9652055.1"
                     /translation="MNKSGFRIIFLGTPDFAVASLKKLVENNWNIVAVVTAPDKPSGR
                     GQKVSSSPVKEYAEQKSIPVLQPTNLKDSEFISRLKTFNAHLQIVVAFRMLPREVWQM
                     PRLGTFNLHASLLPNYRGAAPINWAIINGEKETGVTTFFLRHEIDEGEILLQEKELIS
                     PEDTASNLHDRLMMKGADLVNQTVSLIFERKENPYEQILSGKERNAPKLNKEMCRINW
                     SNSSKNIVNLIRGLAMYPGAWTELNGIQYKIYKASRLKAGKKSEPGSVISDSKNFLHI
                     ATNDGIISILEIQKEGKRKMGIKEFLVGNKI"
     gene            complement(15161..16372)
                     /locus_tag="VGA21_15950"
     CDS             complement(15161..16372)
                     /locus_tag="VGA21_15950"
                     /inference="COORDINATES: protein motif:HMM:NF018743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HEX9652056.1"
                     /translation="MIFLTKLKINKAFLSAYLAAFTILPSNCQHKILSTENVVLPGAF
                     QMDEIKSLLDGKRIGLFANHSALINQTHLLDTLLSLNINVVKIFGPEHGFRGQAADGA
                     KISDEVDEKTGIPIISLYGSRRRLSKGDLADIDVLVFDMQDVGTRFYTYSVDMHFAMQ
                     TVAESNKKFVLLDRPNPHGDEVDGPVLDTAFRSGIGQNRIPVLHGLTLGEMAQMINGE
                     GWLKDGIQCDLSIVKAKNYDHRMPYSLPIRPSPNLPNDHAVSWYPTLALFEGTWISVA
                     RGTDFPFQAIGCPDSTFGNFSFTPRSIEGISTNPPHLGITCYGIDLRNITPPKGLDLQ
                     YLLRFYYIVEDKESFFRSYFNLLAGTDVLMQQIKAGMSEDEIKETWKPDLDQYKKIRA
                     KYLLYPDHSNE"
     gene            16396..17613
                     /locus_tag="VGA21_15955"
     CDS             16396..17613
                     /locus_tag="VGA21_15955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009184456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX9652057.1"
                     /translation="MNLSYFISKRISKPAANTFSSTIYKIGIASVGISLATMILSFCI
                     LLGFRGIIEEKIISFGSHLNVTRFTLTSSYLENPISTGRDIYVNYRQYPFIDHIQTYS
                     YKPALLKTEEEVLGAFLKGVAPDFNLKRFNANMAEGGFVEFDDSAASNDVVISKIIAN
                     KLKLKVGDPLIAHFFQNPPRVRRLNVKGIYETWIEEFDEKVILVDIKLIQRLNNWPDS
                     LVGGFEVFIKDFNQLEANEETLYDNIDSDLYVEKVTDQYQQVFEWLGLIKQNMLIFLV
                     LVLFVASFNMISIVIILIMERTPMIGMLKAMGAPDKQIRDIFTYNGWKLILWGLLIGN
                     ALGIGLGMLQDKFRLIPLNPENYYMSYVPISWNWPIIIGLNFSVSLLILLVLFIPTAI
                     ISRIQPIRSIRFD"
     gene            complement(17588..18349)
                     /locus_tag="VGA21_15960"
     CDS             complement(17588..18349)
                     /locus_tag="VGA21_15960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013455853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HEX9652058.1"
                     /translation="MKSLVIIPTYNEIENIELIIRSIFSLKTTFDILIIDDGSPDKTA
                     EKVKDLQNEFNSKHQQLHLIERKEKLGLGTAYLKGFEFALANNYDYVFEMDADFSHDP
                     KDLKRLLRAARVERYDLVIGSRYIEGVNVVNWPLGRVLLSYIASLYTRIITGMPVHDA
                     TAGFKCYKIKVLRTIDLSAIRFVGYAFQIEMKFNAWKHGFKIKEIPIVFTDRIRGDSK
                     MSIKIFNEALFGVIQMKVGSLFKKYKKVNRTVSTV"
     gene            complement(18418..19806)
                     /locus_tag="VGA21_15965"
     CDS             complement(18418..19806)
                     /locus_tag="VGA21_15965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008626779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HEX9652059.1"
                     /translation="MKQYIDLIEQTFEFPQKEFRVENNELYFNDVPLMKLIKEYGTPL
                     KVTYLPKISENIKIAQKLFGDAMAKFNYKGKYLYSYCTKSSHYKFIFDEALKNDVHIE
                     TSSAFDIPIVRKLLKEGKITKDTYVICNGFKRPLYRQYISELLNEGFKNCIPILDDLN
                     EIKFYDENIKVPYDLGIRLAADEEPKFEFYTSRLGIRYTDVRDLYNEVIAPSPTANLK
                     MLHFFINTGIRDTAYYWSELSRFMYKYCELKKICPTLDTVDIGGGFPIKSSMHFDFDY
                     KYITEQIIENIQWICKKNHVETPSIITEFGSYTVAESGALLYQILNQKLQNDKELWYM
                     IDGSFITHLPDAWGLNQKFIMLAVNKWAENFHRVNLGGLTCDSMDYYNSEAHSSEVFL
                     PKIENGEPLYIGFFHTGAYQDTLGGVGGLQHCLIPAPKHVLIDKDAKGKIKTRLFRDE
                     QTSSSMLKILGY"
     gene            20004..20915
                     /locus_tag="VGA21_15970"
     CDS             20004..20915
                     /locus_tag="VGA21_15970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013455359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HEX9652060.1"
                     /translation="MKLQVRDEISPLRSVIVGSAFDFGGTPLYKEAYDPKSKENIRNG
                     TFPTEEDLLEEIDGFAEKLKAMGVYVYRPQNVEGINQIFARDIGLVIEDFFIKANIVA
                     DRTKEIRGILHLLKKIDSRKVVQPPVKVRIEGGDVMPWYDKIFIGYSKYADFQKFIVS
                     RTNEAGIEFVKQLFPDKEVIPFELNKSDNDARKNALHLDCCFQPIGKGFAIMYRDGFK
                     NKREANYLVNFFGQPKMIFIDQQEMYDMCANIFSVNPNLIISSKNFVRLNKELRDRGF
                     KVEEVKYNETAKMEGLLRCSTLPLRRS"
     gene            20945..21907
                     /locus_tag="VGA21_15975"
     CDS             20945..21907
                     /locus_tag="VGA21_15975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009583184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase-related protein"
                     /protein_id="HEX9652061.1"
                     /translation="MIRPVQFRYNEQTAENNFYQKDPDNLDDETAGEKAQREFDNFVQ
                     VLRSKGVDVLVVEDTFSSDTPDSIFPNNWVSFHEDGTVVLFPMFAPNRRRERRKDILN
                     DILPGRGFAVKKIIDFSDFEKSERFLEATGSMVMDRDNRIIYASISERTDPGLVDEFC
                     IKFGYKAVKFHSYQTYQGERLPIYHTNVMMTLADEFAIICGDAVDNKEELKDVVSNLK
                     KTGKEIIYITEEQTRKFAGNMLQVINKDGKKLVVMSSAAYHSLNPEQVRQVQHYGELV
                     HSDLKTIETCGGGSARCMMAEVFLPMVDFSLDKPSELINLPSSN"
     gene            21918..22892
                     /locus_tag="VGA21_15980"
     CDS             21918..22892
                     /locus_tag="VGA21_15980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011585322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008612 - peptidyl-lysine modification to
                     peptidyl-hypusine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase family protein"
                     /protein_id="HEX9652062.1"
                     /translation="MPDGPVTAFIEKHYLHFNAAALVDASKAYIKHLEEGKKMMITLA
                     GAMSTAELGISLAEMIRQDKVQIITCTGANLEEDLMNLVAHNSYKRVPNYRDLTPKEE
                     WELLEKGYNRVTDTCIPEEEAFRRLQKHIFKIWKEAEESGERYFPHEYMFKMLLSGVL
                     EQYYEIDSKNSWMLAAAEKNLPIIVPGWEDSTMGNIFASYCIKNELTPSTMKSGIEYM
                     TWLADYYTRNAGTEGIGFFQIGGGIAGDFPICVVPMLYQDLKRTETPFWSYFCQISDS
                     TTSFGSYSGAVPNEKITWGKLGIDTPKFIIESDASIVAPLVFAYILGW"
     gene            22894..>23450
                     /gene="argS"
                     /locus_tag="VGA21_15985"
     CDS             22894..>23450
                     /gene="argS"
                     /locus_tag="VGA21_15985"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009583189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEX9652063.1"
                     /translation="MKTLRIEDQIKEGITLAFNQLFKAEVKSEEIKLQPTNKEFEGDF
                     TFVVFAFLKETKKSPEESGKMIGEFIMNHTGLIDSYNVVKGFLNLVISGEVWISTLKN
                     IANNDQHGFKQRNGTQVMVEYSSPNTNKPLHLGHLRNNFLGYSVAKILEANGNTVHKV
                     QIINDRGIHICKSMLAWIKFGKGETP"
BASE COUNT         7112 a         4584 c         4839 g         6915 t
ORIGIN      
        1 taaagctaca gccattccga tattgatatc tttcttcctg atgattttgg ttccatccac
       61 ctggatgttg atcattggat agtcttttat cgcccggatg acggcgtcaa tcatgatggg
      121 tgtgaatgtc aggttatcgc cttctttttg cttgaattcg tttttatatt tatttctcca
      181 ggccacaata ttggaaacat ccgcctccac aaaggaagtg acatggggag aagtacgctt
      241 tgattcaacc attcgttcag caatcatctt acgcatgcga tccatctcga tgatctcatc
      301 ttgtccggat gagattacag agaatgatga ggtctcctgg taagcaggca taagtacacc
      361 gccagacttg cgctgttcaa catacgccag gatatcatat ttcgtaagcc ggcccttttt
      421 acccgaaccg ggcacagctt ccagctctat taaaggaatt tgctcctgtt tggcaatact
      481 cttcaccaat ggagagtaaa accgggttga tttctgtcca ttggaaccat tatttgaaat
      541 cgtttctact ggtgctgcga caggttctgg cctggattct tgctttactt cttttttttc
      601 ttcccgggcg ggggctttag ttgtgacggt atcgtcacct tcggttacaa taatagcaat
      661 gggtttacca accggtacaa catcgccttc tttggccaaa atttctttaa gaataccagc
      721 atgcgttgca ggaacttctg tatctacttt gtcggtggct acctccagaa cggattcatc
      781 ctgatcaatt ttgtcacctt cttttttcag ccaggttaat accgtggctt ccataatact
      841 ttcgcccatt ttgggcatca acatttcaac taaagccatt aaaaaagtta attattcttc
      901 aaatttagca tatacaggca agtattcaat cgatttcgag caatctttgc cacaacataa
      961 acagagcagc cagtgtagaa aaatggatat tggcctctct gtctccgcca aacgaaaatc
     1021 ttttggcgtg agtaccccag ggaccggcca atccaatcca gactgtcccc accggtttgt
     1081 cttccgtacc gccaccaggt ccagctactc cactcgttga gattccaatg tctgcttcaa
     1141 acaatgattt tacattatca gccaattgta ttacggtatt ttcacttact gctccttccg
     1201 taaaaagggt ctctttgttc acgttaagta tcgcactttt aaattgattg tgatagggaa
     1261 ttacacatcc ctggaaataa tctgaactgc ctgccacgga agttatggtt ttgctcaggt
     1321 tgccgccggt acaactttcg gcacaggcta ctttcagttt cttgctcctc aggctattcc
     1381 caacagcgcc ggctaatgta tccttatcat atccataaat atgcacgccg attaattgct
     1441 ttaacttttc tatttcaatt gaaataagtt catcgatttc agattccctg gcaccaatac
     1501 aggttagtcg caattttaca tcaccaaggc cagggagata ggcaagtttg agttcaagtg
     1561 gcaaattatt ttcccattct tttattttat cagaaagata ggattcgcca ataccaatgg
     1621 ttttaattat tttatgtttg ataaatggag tgggaaaata ctctttaatc cctggtataa
     1681 cttgccccct cattaaatgt ttcatttcac ggggaacgcc gggcatggat acaaatattt
     1741 ttccatcttc ttcgaaccac atccccgggg cagacccttt cggatttgta aggggtttac
     1801 atttttcagg caactcagcc tgatgcttgt tggtctcaga gagttcccta ccgaattttt
     1861 tgaagagttc agttatatgt ttgagctgat catcatttag cactaattcg caattgaaga
     1921 atttggcaag acaaggtctt gtcagatcgt cagaagtagg tcccaggccg cccgtaatca
     1981 aaatcagatt tgccctggat ttagcttctt tgaaaacatt gaggatatct tcctcattat
     2041 caccaacggt ggttcgccag cgtgttttaa tccctattaa atccagttcc gagctgatcc
     2101 attgtgaatt tgtgtcagaa atttgcccgt aaagtatttc atcgccgatg gtaattattt
     2161 cggcataaac attgttcata aaataacttt tgaatcttat tgatttagga aatgtagata
     2221 aatctcatca atttttcttt gaaaatcctc gaaactatga agacgttcaa aattttgatt
     2281 attgctacgg gtttgtgcat cagttgtccc gcgagtgctt accaggttaa gggtctcttt
     2341 aaaaaagcgg aaaaggcatt gaccaactcc ggtttatcgg aagaagaaat tggaaaaggt
     2401 ttgcgcgaag ctttgataaa aggaatttcg gtcggttctg atctggcaag ccagctggat
     2461 ggttacttcg gaaatccatc tatcaaactt ccttttcctc cggatgctca aagggtggaa
     2521 accacgcttc gtaatatggg ctttggggca aaagttgatg aattcatttt gacattaaac
     2581 cgtggagcgg aaaaggcagc caaagaatct aaagacatat ttattgaagc aatcagagca
     2641 atgactattc aggatgcatt gggtatttta aaaggggaag atgatgctgc cactcagtat
     2701 ctgatgcgaa cgacttcaca aactcttttc agaaaatttc aacctataat aaagagagcg
     2761 ttagacgagg ttaatgcaac ccgttattat gccgacttgg taaatactta taataaagtc
     2821 ccgcttgttc gaaaagcaaa tcctgatctg gatgaatatg caaccaataa agctattgaa
     2881 ggtttatttt atttgattgc tatcgaagaa ggaaaaatca ggaaagaccc attggccaga
     2941 acttctgaca ttctcaaaaa agtttttagt caacaagatt aattctcaac ttgtatgcgg
     3001 aaagatttct gatgctgaga ggaaattaca tttaccaggt agataccatt tgaagtattt
     3061 tcattaagca aaatcattac tccctccggc atttttgtga tttgaaattc gattcctttt
     3121 cctgttaaat ccaaaatttc aactttacct ggcgtgagtc ctgatataaa gaattgatta
     3181 tttctaaccg gattcggaaa atagataaga gttccatcac tttcatcaaa ggaggtgata
     3241 tccgcaacca gtaccgagcc aagagttgtc gaattgcagc caagagaatc cgttactgta
     3301 agttgaacgg gataaagtcc gggtagcagg aagcttaccg taggatttat gactgaggag
     3361 aaacccgcgg aactgaaatc ccaggaatag ctggataaga gtttttcttc cgcctggaat
     3421 tctatttggc tgattccgtt actcatgagg gtatccttca gtggtgtaaa accggaattc
     3481 ggaaaactaa ttttaatttc aaatttcgcc ggtgcgcttt ccaggagcga gtcaattgaa
     3541 gtaaaataaa aaatactgtc gcgatcaaga tttgttagtt ccaggtacct tcctttttgg
     3601 ataatttcat ttttgttatt atagaagaca aaaatggatc ccggttcggg tatcaacgag
     3661 gcaaaactat tttgacaaat tgtaatcatt tcctctttag gtgctggaga aatctttaat
     3721 gggatagatt taaccaggct gtcaagacat ccaaattccg aagcgactct ttgttttatt
     3781 agcaactcat ttaatccgct gtaatttaca atctgagtat caccggatcc caggtaagta
     3841 tctcctaaat accattcact cgaaacgccg ggagtttctg agttcagctg caatactgtt
     3901 ggcgactcaa agtgggtagt gtcaattgag aagtcaaacc cagtcattaa attttcgatg
     3961 atgacatcaa cccgggttgg cagactttca ggtttaaggc ccacctgggt gagataaaaa
     4021 gtggtatcgc taatcaagtc atcagtgaaa atttcagatc ccgatgcaac cggaggggag
     4081 atagaatcaa cataaagatt tagaatttta cctgaatcag agaaagaaag attttctcca
     4141 tagcaaatga taataaactc cgggaatacc ggtttttcat aactttcaat tacagaaaga
     4201 tgcttagtta tgctttcctc acaaccgaga ttatcggttg atgtaagttc aatattataa
     4261 gtccccggaa gggaatattc gggttttgca tcgttgttta tacttccata tccatttcca
     4321 aaatcccagg ttcgggaaat actacctgtc gtaatatcct gaactgcaat actgcttttt
     4381 tctcctggat ggagcaccag ggaggcagaa gacatgataa agtctacatt agcataatgc
     4441 aggtccacct gaattgaata agactcttct tcaagctcat taatcaggga agtgatataa
     4501 taggtttgct gattctgaag agcgggagta ataaattcat ttcctgtgaa caataagtca
     4561 gtatgttgtg catcactgaa cagtttaaag tcattcagat ttgcaggatt tattaccgcg
     4621 ctctctcctt cacaggccag gaaagtttct aattcaggat ggtattgaga ataggcatca
     4681 tatctggttt cggccagact aacttttgct tccaggtccg tcagtgatgt tccgaaaata
     4741 atggaaaaag caatcgtgac actttccagt ggagcaataa ccgggattac ggcaccgaat
     4801 acaatggcta catcatttcc gctgccgtta actccagcat cggtttgagt aaggccttca
     4861 ttcataaatt gaaatttttc cgtatcacca aaggtgattc caatatcagg tacgttgccg
     4921 ttgaaattgc caaaatctat accacgcgat acggggtttt ggttggtgag taatgcaatt
     4981 ccgaaggctt cattgtcaga gtatatatat ccaagtttac tgtcggaaga aaaactcgtc
     5041 ttattttgag tataatcctc aagatcgatg tcaaggaaca ttccaaaatc aaaaccatta
     5101 atagattcag gatttaaatt tgttacacgg tattccagaa tcatgaaatc ctgatcttca
     5161 gcatttttcc aacctaacaa tttctcatcg attcggattc ctattgctgt agcagaaggc
     5221 ttctcttcaa tccgcaatct gtaatcaata tctgagtaag gcgtttgatg ggattttaca
     5281 aagcgtatcg tttcaaaatc atcatcttga gtataggtaa caaaatcatt gatgactgtt
     5341 cctgatacat gggatgcatc tgttgccgcg attatacccg cgtgccaccc ataatcaata
     5401 ttctggaatc ttagcgagtg accgttgtaa taatcgtcaa aatatccaag atgtcctgtg
     5461 ctgtttgcag tccatgtaat tttttcatta ttgaattcaa taaagtcagg accatttctg
     5521 aatggcagca tttgaaaatc aacataagct ccgctttcga aagagaaata gagttgcaaa
     5581 aattcggagg gtggagttaa ttcatctatc ttaaccgaga aagaataaga tatgctggtt
     5641 tctaaagtat ttaatgaatt cagggttatt gattgagaca ccatcgtaat attactgcta
     5701 agagtattca gggctatggt cagcggggaa ctaatgggat caagaaaatt tttgaaaaca
     5761 acctcaaagt gcaatgagtc gtcataaaaa ggctgaataa aatccggttg aataaatgca
     5821 cggaccacag gtccagcgga tgattccagg gcttttcgca tatttacccg gccttttccc
     5881 agtaagccaa agaaattctc attaccagga acagaataaa tatcatccgc agaaacccgg
     5941 attttttcca ttatttgcag cgcatttaag agtggaaaac tttgtcttac cagtgccgcc
     6001 agacctgaaa caagcggtgc agccagggaa ctcccccaac tattcgcata accgttggta
     6061 ggttgagtag tgtaaatatc gatcccgggg gccgacagat caatatattc gctaattgta
     6121 ccccagtcag ttttttgatc agaggaattg gtggatccta cagataaaac gttatcataa
     6181 gaagcggggt agaaggccag atgttcattg gtatttcccg cggcggcgat tatcactgca
     6241 tcaaaatcta tggctgcgta attgataatg tcctgttcaa attgtgagta ttctcctgca
     6301 tcaccccagg aaaggttaat aacttttgct ccttgttcaa ctgcatagac gattgcttca
     6361 tacgcgccgg tcagctcatt cagagcggta gagtgaattt tgataggcat gtattttgaa
     6421 ttgtaaccgg ttccggcacc acccaaagta ttatttgcgg cagcaaatcc tgctccggct
     6481 acaattgttc catgagcttg cgacaacgag gaagggtcat tatcattatc tccaaaatcc
     6541 cagccaatga agttatcaat gtacccatca ttatcattat caataccatc aacaggatca
     6601 ttgtcatttg tgtagaaggt attcgccaaa tcttcatggc ttaaatcgaa tccggtgtct
     6661 atgattgcgg tgataaccgg atttggagca ggggaaatta agtcccagcc aaggtatgca
     6721 gacattgccg caagatgcca ttgctcagat cccggttgtg ccgcgggatc atccggcaca
     6781 aatagaggtc tgtaggtctt ttctttttca agaatttcaa tatcagggtt ggatttaaga
     6841 attttaatta tttccccggt tgaaaacttt gccttgatcc tgtggtaatt ccgcaatcgt
     6901 gatcctggtt ttctccctgg aaagacgggc tccatttcat tggcaaaacc tcttgattta
     6961 agccactggg cgactgcctg gatcttccct tcttttattt ttaggataaa agattcttct
     7021 gttgaattat ttgattgagt aaactgcagg tgagtgagtg atactaaaag aaagaaagtg
     7081 agcgaagatt tcattaaaat tccatttatt gtctagtgcg ggtctgtaga taatgcctgc
     7141 tataataaat ttgaaaaaac atatatttca acaaaaatac agttaaaagc ttattattaa
     7201 ttagataagt aatgttatca taaaaaaggt gaggaaaaat taattggaat gttaatattc
     7261 aggttctttt acaactttcc agcgtccagg aacatcctta aatcagaaaa caattagcgc
     7321 aagcaagtac tgtagtacat tttttgctcg ttaagtaacc aataacaaca aaagacagta
     7381 ttagtaatta ccaggtaatc cgtcaaaatc ccaaaataac cccaaagtca tgaataagcg
     7441 aatactaatc ggagcaggac tcataatatc ggttgtagta cttgtactca tttaccaatc
     7501 cacaaaagga agttccagcg aagatgtaga agtccttaca gaagcccagt ttggagactt
     7561 cataattgat gtgaccacca caggagaact ggaagccaaa agttctgtca ggattaccgg
     7621 gcctaacgga ctccagcagg ctcagatctg gcagataaaa attgatgata ttgttgaaga
     7681 aggcacactt gtaaagaaag gagattacat cgctaagctg gagcaaaccc aactgatgga
     7741 taagattcag caggaacaaa atgatctttc ccaaagtgac tcaaaataca ccgagatgaa
     7801 actggatact gccctcgaac tacgaaaggc ccgcgatgag ctaattaatc tggagtatga
     7861 aattacaacc aagaaaatta ttgttgaaca atcaaaattt gaaccgcctg ccacccagac
     7921 agccgcgcaa attgaatttg aaaaggcaga acgtgctcat aagcaggcgt ttgacgggta
     7981 taaattaaga aaggaaaaag ctgtcgcaca gatgcagcaa gcgtgggcag agcgtcagga
     8041 agaccaaaca agagttgatt ttttgatgcg gctggcccag gaatttaccg ttaccgctcc
     8101 agaagacggt atggtaattt atgccaggac ctggaatggt accaaaaaag gagtcggaag
     8161 tacggttcag gcgtgggatc cggtagtggc cacgcttccg gatttgaata aaatgatttc
     8221 aaagacgtat gtcaatgagg ttgatattcg agtggttaag gttgggcaaa ctgttcaggt
     8281 tggtttagat gccttcccta caaaaaaact gacaggaaaa gtgatcaccg ttgccaatgt
     8341 aggcgaacaa aaacccaatt ctgatgcaaa agtcttccag gtagatatac tcattaatga
     8401 atctgataca acgcttaggc ctgcaatgac caccagtaac actattatcg cggaagttgt
     8461 tccaggcgta gtatttgttc cgcttgaaag cctgcattcg cagggagaca ccttaaccta
     8521 tgtcatcaaa aaagatggac taacattttc aaaacaagaa gtaaaggttg ggaaaacaaa
     8581 tgctgacaaa gctgtaattc tggaaggagt gaagcaggga gaaatagtgt atttatcgaa
     8641 cccgacaggt tatgaatcta aaagacttat aaaattgcca gaaaagggaa cgtcggtagc
     8701 ttcttccaat tagtatgact aaatatcttt tcaattttca tattgcggtc gaatctgttt
     8761 tatccaataa atttcgttca ctgcttacgg ctttgggaat aatttttggg gtggccgccg
     8821 taattgccat gatggctatt ggaagtggag ctcaaaagga aattctcgaa caaataaagt
     8881 tggtcggtgt aaacaacata gtaattacac ccgttgtaga acaaaaggaa gaagttcttt
     8941 cagatgaaac cgctgccaaa gagaaaccca acttctcgcc gggattaact ttgtacgatg
     9001 ctcgaagtct tgaaaaaata attcccggaa tcactcactt gagtcctgag attgtgctgg
     9061 agacaaatat aataaagaat ggcagaagaa ggtcagcgaa attagttggg gtggacaaca
     9121 gttatttttc tgtggcaaat tttacttttc aaggtggtta tatttttagt aaggaacaac
     9181 ttgaaatggg ggaagcagtc tgtataattg gcaagggagt tcaggcaaag tttttcagcc
     9241 aggaaaaccc tcttggaaaa atgctcaagg taggaaagca ctggctcaga gtgataggtg
     9301 tacttgatca gcggtttatt tctgaaaaaa gcttaaccaa tctgggcata agagattaca
     9361 atatggatgt gtatgccccc cttaataccg tattattgcg atataagaac aggtcattaa
     9421 tcacggaacg tttactactc cgggctcagt ttcaggaaga agaaaatgaa gaaaataaga
     9481 aagcggaaaa ttatcatcaa atagacaggc tggtaattca ggtggaaaag acagaaaaac
     9541 tcactggagt tgctgaagtg gcgtcaaagc tgttgaaaag gcggcattat gaaatggttg
     9601 attttgaaat taccattccc gaattattgt tgaagcagca acagcgcaca aaaagtatct
     9661 tcaattttgt tttaggcgcg attgccggaa tttccctact ggttggaggc atcggtatta
     9721 tgaacataat gcttgcctct gtgatggaga gaataaagga aattgggtta agaatgtcga
     9781 ttggtgcgaa aaagcgggac atcattgcgc aatttctatt tgaggcggtt atgatatccg
     9841 tttccggagg ctttgttgga gtgtttttgg gggttgtatt tgcgattttg gtttccaact
     9901 ttgctgatat agaaacttct gtcactttta tttccatttt gatttccttc ggagtggcag
     9961 catccatagg aattattttc ggaattactc ccgcacaaag agcagctcag caagatccca
    10021 ttgtgtcact tcgacacgaa taaataatat catagtttca acggcgagtt tttgaaagac
    10081 gactattagc agaaaaataa ttaacttgcg cacctcattt aactgataac tataaatttc
    10141 caatggattt atttgaaaaa cacaagtcta ttatagaaaa ggcaatctca gctttgcata
    10201 aaagggaatt ctttgcccaa tatccggaac atcccggcgc ctatccggag gatgctgatg
    10261 caaaaggcag agaaaaatat atgtctcttt ccggaaatta ttttgacgag ataagtgctg
    10321 agggatcgga tggatggata ggagtggaag taagtcctta tactcaagag gaaatcaata
    10381 ttaagtatcc cgcattttca gtggatacat taataagtaa tgccaacaaa agctttggtg
    10441 catggcgaaa agcttccccg gagactaggg caggaattct cgtggaaagc ctggaaatgt
    10501 taaaagagag attctttgaa attgcctacg caaccatgca tactaccggc caggggtata
    10561 tgatggcctt tcaggcatcc gggcctcatg ctgctgaccg cgctctcgaa gcaatagcgg
    10621 cgggctatga agaactaagc cgatttcccc ggcaggcaac ctgggataaa cctatgggca
    10681 agttcaacat caaacttgaa aaagaatgga aggcggtgcc aaaagggata ggtgtcgtaa
    10741 ttggatgttc cactttcccc acctggaatt ccgttacagg tttatatgca gacctggtta
    10801 cggggaatcc tgtcattgta aaacctcatc ctggagcagt tttacccatt gccatagttg
    10861 ttgcagaaat tcagaaagtg ctcagggaaa atggatttga tgcgaatatt tgccagctgg
    10921 cggtggatac ttttgaaaag actatcacta aagaattagc ggagcatcct caggtgaagc
    10981 tgatagatta tacgggaaat tctgaatttg ggtcctacct ggaaggatta tccggaaaat
    11041 gcgtttttac tgaaaaaacc ggggtaaact ccgtcattat tgatagtgtt gaaaacgcgg
    11101 atgccgtaat tcagaattta atcttttcgc tggcgcttta ttccggacaa atgtgcacag
    11161 ctcctcaaaa tttctttatt cctgaaggag gtataaatac accagatgga attattactt
    11221 ttgatgagtt tgctgaaaag tttgttgctg caacttccgg attggcggat aatcctaaag
    11281 ccggaccttt tgtgcttgga gccatccaaa atcccaaaac cagtgagaga gtgaaaactg
    11341 cggaaaagat cgacggcaaa gtatggttga aaagtaaaac cattgagaat ccacagttta
    11401 aaaatgcacg tactgcaaca ccggcaatta ttgaggttaa tgcatcgaat aaagatagtt
    11461 tcagcaaaga actttttggc ccgatagctt tgctgataaa gacaaagaac actaatgaat
    11521 cagttcaact tgcaaaagat atagcgatga aatttggggc cattagctgc ggcgcttata
    11581 ccaccaatcc tgaaataaaa gaaatgatca aagatgagat gtcgcaagtc gctactccgg
    11641 tttcattcaa ccttgttggt ggaatttatc taaatcagca tgccggattc agtgactttc
    11701 atgttacggg aggaaacccc gcagggaatg catcttttac aaatccggag tatgttataa
    11761 agcggtttgt gtgggtagga ttcagagaac cggcaaaagc ttgattttaa aagaaccaaa
    11821 gtctaatcgc gagcactatc actgtaacaa acaaaactct ttttatccat ttgtctccct
    11881 tctttacaga ccagcggctg gcaatccagc cgcccgctga attgccgact gccaacgtga
    11941 gaccccaggt ccagttgatt ttatcttcaa tgatataaac aataagggca gaagacgtat
    12001 acatcaacgc ggcaagaact ttgagactgt ttgttttcac caggctcagc ctgttcacaa
    12061 tagttacacc ggcgattatc agaaatccaa cgcctgcctg tatgaatcca ccataaattc
    12121 caataaaaaa gaacatgatt actccataaa tctgatgtct cttgtcaagt ctttcttttg
    12181 tttcatctga attgccactt cgatccaaaa tggtccatac aacaacggca accataatga
    12241 ttgcgagaat cttgttaaag agatcgctgt caatttctac tgagattttt gcgccgatta
    12301 ccgcaccggt aattgaagca aggcctaggt atattccata gggatagatg aaaacacctt
    12361 tacttttgaa tcctgcaact gcaaaaatat tctgaaaaat aatggccact ctgttggtag
    12421 catttgctac gacggagtcc agtcccatga aaatcattag tggaagagta agcaacgaac
    12481 cgccaccggc aacagtattc agaaaacccg cgaggatgcc ggtgagaata agaaagatgg
    12541 cctgataaat ttccatgaat caaaatcact tccgccggga ttttttaagc tgtttccctc
    12601 tttcctggtt gaagcgacga tagtttaacc aattgataaa caaaaaaaga cttgagaata
    12661 ggattaagcc gacaattccc ttcttttgat cttcatccgc ttcagtaaat ccatagtaag
    12721 cgaagagcag ggataacgca aatacgatga aggatatgta gaatcctaaa acagtaaatt
    12781 gttttcgcat ggtttcaaat ttgtcaggca agttctttca gaacatcttt ggtaactctt
    12841 tctccggtaa ttatcaatac tacattctta tcttccaaat cctgatgtct tttcaaaagg
    12901 ccggcaacag taagagcggc ggcaccttca atcagcatat catgatatga aatcattatt
    12961 cttattgcat ccaaaatttc ccattcctta accagaaccc aatcatcaat taccctggag
    13021 caaattggga atgttatggc tcttttttca attccgccgg cggttccatc tgccaatgtg
    13081 gattcggatt tcagcttcag gattttgcca gccataacag attcatacat cacctttgag
    13141 ttgaaaggct gacatccaat gatctgagta agaggccggg ctttcttaaa ataggaaccg
    13201 accccggcag ctagcccgcc cccgccaacg ggtataaaaa cggtatgcac attgagaagc
    13261 tgatcatgaa tttccaatcc caacgttccc tgaccctgaa ttacactgag attgttatat
    13321 ggagacacat aaggcatttc cttttctctc ccaaaacgct tggcttcgta ctcagcttcg
    13381 agtgtatcgt aaccaaattg aaccacctga acgggatatg acttcaacgc gtcaagtttc
    13441 gcttttggaa cagtaacagg caggaagata atgcctacaa aacccatttg ttgagctaaa
    13501 taagcaaatg ccgccgcatg atttcctgag gaggctgcga caaacccgtt cttggttgat
    13561 agtttcgtag ttaaaatctt attgcaggct cctctaatct taaatgaatt tgttacctgc
    13621 ctgttttcca atttaagata taccttgcag ccacactgac tgctaagata ttccgaaaac
    13681 tctaactcgg ttttaacaat gtaatttttg atgcgggtcc gagccgctct gattttcatg
    13741 atcatgccac tattttttct agtaaaacaa aattataagg atatgcgtgt ttttcatcag
    13801 gtaaattctg tatcctggaa atttctttcc actctgcttt ataataatcg gggaagtagg
    13861 tatctccgga aaatttcgcc ttaacttcgg tgatataaag ccggtctgcc ctgttcatgg
    13921 attgccggta aatttcacca ccaccaatta caaatacctc ctgctggtga gcttctgcag
    13981 ccagatgaaa tgcttcttcc agggaatgca caacgatggc tcctttggca agaaatttct
    14041 tctgacgtgt aacaataata ttagttctgt ttttaagagg cctgaatttt tcaggaatgg
    14101 attcatagtt ttttcttccg gtgattatat aatgacccaa agtagtgtcc ataaaaaatt
    14161 tcatatcccg tggcagatcc caaaccagtt tattgtcttt gccaattact ttgttctctg
    14221 ataccgccgc gattaacgaa acaatcatat tttatttcct actaaaaatt ccttaatacc
    14281 catctttctt ttcccttctt tttgaatttc aagtatagag ataattccgt cattagtggc
    14341 aatgtggaga aaatttttac tatccgaaat aacgcttccg ggttcagact ttttgccggc
    14401 ctttaaccga gatgctttat atattttata ttgaatgcca ttgagttcgg tccatgcacc
    14461 tggatacatg gcaagtcccc gaatcaggtt cacaatattt ttggacgaat tactccaatt
    14521 aatccggcac atttccttat tcaattttgg tgcattcctt tccttgccgc ttaaaatctg
    14581 ttcatacgga ttttcctttc tttcaaaaat taaagaaaca gtttggttaa caaggtcagc
    14641 acctttcatc attagtctgt catgtaaatt gctcgcggta tcttctggag atattagctc
    14701 cttttcctgg agaagaattt cgccttcatc gatttcatgc cttaaaaaaa aagtcgtaac
    14761 acccgtttct ttttccccat taataatcgc ccaattgatt ggtgcggcac cccggtagtt
    14821 gggcaacaga gaagcatgaa ggttaaaggt tccaagccgc ggcatttgcc acacttccct
    14881 gggcagcatt ctaaaagcca caactatttg taagtgtgcg ttaaatgttt ttaatctgga
    14941 aataaattca gagtctttaa gattggtggg ttgcagtact ggaatcgact tctgttctgc
    15001 atactcttta acaggtgatg aagatacttt ttgacccctg ccgctaggct tgtcgggggc
    15061 agttacaact gccacgatgt tccaattatt ttcaactagt ttttttagac tggcaacggc
    15121 aaaatccgga gttccaagaa aaattattct aaaaccggac ttattcattg gagtgatccg
    15181 ggtataacaa atattttgcg cgtattttct tgtattgatc caagtccggc ttccaggttt
    15241 cctttatttc atcttcactc atcccggctt ttatttgttg catcaaaaca tcggttccgg
    15301 cgagcaaatt gaaataggaa cgaaagaaac tctctttatc ttcaacaata taataaaacc
    15361 tcaataggta ctgcaggtcc aaacctttgg gtggggttat atttcttaaa tctattccat
    15421 agcatgtaat accaagatga ggtgggtttg tagaaatgcc ttcaatagac ctcggagtga
    15481 atgaaaaatt accaaaagtt gaatccggac agccaattgc ctgaaaagga aaatcggttc
    15541 cgcgggccac acttatccaa gtcccttcaa ataaagcaag agtagggtac catgacacag
    15601 cgtgatcatt gggaagattc ggagacggtc gaattggcag tgaataaggc atccggtgat
    15661 cgtagttctt cgcttttaca atactaaggt cacactgaat accatccttc agccaccctt
    15721 caccattaat catttgagcc atttccccaa gggttaagcc atgaagaacc ggtatcctgt
    15781 tttgtccgat tccggaccgg aaagcggtgt ccagtacagg gccgtcaact tcatcaccat
    15841 gtggatttgg tctgtccagg agaacaaatt ttttgttgct ttccgccact gtttgcatgg
    15901 caaaatgcat atccacagag taagtataaa atcttgtacc aacatcctgc atatcaaaaa
    15961 ccaacacatc gatatcggca agatcccctt tagaaagcct gcgtctggat ccatagagtg
    16021 agatgatggg gattccggtc ttttcatcaa cttcgtcact gatttttgca ccatctgcag
    16081 cctgccctct gaaaccatgt tcaggtccaa atattttcac cacattaatg ttcagggaga
    16141 gtaaagtatc cagcaaatgg gtttgattta ttagtgccga gtgattggca aacaacccaa
    16201 ttcttttgcc atccaaaaga gattttattt catccatctg aaatgctccg ggtaaaacaa
    16261 cattttcagt acttagtatt ttgtgctgac aattagaggg aaggatagtg aaagcagcta
    16321 aataagcaga taaaaatgct ttatttattt taagtttggt tagaaaaatc atcgtgattc
    16381 aaaattaagg aaaatttgaa cctgtcctat ttcatatcga agagaataag caagccagcc
    16441 gccaacacct tctccagtac aatatataaa atcgggatag cttcagtggg gataagcctg
    16501 gccacaatga ttctctcatt ttgtattctg ctggggttcc ggggaattat tgaagagaaa
    16561 attatcagct ttggttcgca ccttaatgtc accaggttta ctttaaccag ttcatatctg
    16621 gaaaatccta tctcaaccgg cagagacatt tatgtgaatt accggcaata tccattcatt
    16681 gatcatattc aaacttacag ttataaaccg gcattattga aaacagagga agaggtactt
    16741 ggagcttttc tcaaaggcgt tgcccctgac tttaacctta aaagatttaa tgccaacatg
    16801 gctgaaggag ggtttgttga gtttgatgat agtgcagcca gtaatgatgt tgtcatcagt
    16861 aaaataatag cgaataagct aaaacttaaa gtcggcgacc cactgatagc tcactttttt
    16921 caaaatcctc ccagggttcg aagacttaat gtgaaaggta tttatgaaac ctggattgag
    16981 gagtttgatg aaaaagtcat tcttgttgat atcaaattaa tccagcgatt aaacaattgg
    17041 ccggattcac tggtgggtgg ttttgaagta ttcataaagg actttaatca gttagaagct
    17101 aatgaagaaa ccctttatga caatattgat agtgacttat acgtagaaaa ggtaactgat
    17161 caatatcagc aggtatttga atggctgggg ttgattaaac aaaacatgtt aatctttttg
    17221 gtgctggtat tatttgtggc ctcattcaac atgatttcaa ttgtcatcat tttgatcatg
    17281 gaaagaaccc cgatgattgg catgctaaaa gcaatgggtg cgcccgacaa gcagattcgg
    17341 gatattttta cttacaacgg ctggaaatta attctctggg gactgctaat tggaaatgct
    17401 ttgggcatag ggcttggtat gttgcaggat aaatttaggt taattccatt aaatcctgag
    17461 aattactata tgagttatgt tccaatctca tggaactggc caataataat tggattaaat
    17521 ttttcggtga gcttgcttat cttgctcgtc ttatttattc caaccgccat catcagcagg
    17581 atacagccta tacggtcgat acggttcgat taactttctt atacttttta aaaagtgaac
    17641 ctactttcat ttgaataaca ccgaacagcg cttcattgaa aattttgatt gacattttgg
    17701 aatcgccgcg gattcggtca gtaaatacaa tgggtatttc ttttatctta aatccatgct
    17761 tccatgcatt gaatttcatt tcaatttgaa aagcataccc gacaaaccta attgcggata
    17821 aatcaattgt ccgcaatact ttgattttgt agcacttaaa accagccgtg gcatcatgaa
    17881 caggcatgcc cgtaattatc cgggtataaa ggctggcgat ataagaaagc aatactcttc
    17941 caagaggcca gtttaccaca ttaactcctt caatataacg tgaaccgata acaaggtcgt
    18001 atctttcaac tcttgccgcg cgaagcaatc tctttaaatc ctttggatca tggctgaaat
    18061 ctgcatccat ctcaaataca taatcataat tattggcaag agcaaattcg aagcctttca
    18121 ggtaagccgt tcctaatccg agcttttctt tcctttcaat aaggtgtaat tgttgatgtt
    18181 tcgaattaaa ttcattctga agatctttca ccttttctgc ggttttgtcc ggagaaccgt
    18241 catcaatgat caaaatatca aaagttgttt taagtgaaaa gatagaacga attataagtt
    18301 cgatattttc aatttcatta taggtcggga taatgacaag gcttttcact cggagagatt
    18361 ttttgaattc caaacttagg attttaatca agatttttga gtaaaaaacc ggggaattta
    18421 gtatccaaga atctttagca tgctgctgct tgtttgttca tccctgaata atcgggtttt
    18481 tatctttcct tttgcatcct tatcaattag cacatgtttt ggagcgggaa tcaggcaatg
    18541 ctgaagtccg cctactccgc ctaaagtgtc ctggtaagca ccggtatgaa agaatccgat
    18601 atacagtggt tctccgttct caattttagg aagaaatacc tccgaagaat gagcttctga
    18661 attatagtaa tccattgaat cgcaggtgag gccgcccaga ttaacccggt gaaaattttc
    18721 agcccattta ttaaccgcga gcattatgaa tttttgattt aacccccagg catcgggcag
    18781 gtgggtgata aatgaaccat cgatcatata ccaaagctcc ttgtcatttt ggagtttttg
    18841 gtttaaaatt tggtaaagca atgcaccact ttctgctacc gtataactgc cgaattctgt
    18901 aatgatgctg ggagtttcaa catgattttt tttacaaatc cactgaatat tttcaataat
    18961 ttgttctgta atgtatttgt aatcgaaatc aaaatgcatg gaagacttaa tcgggaaacc
    19021 accgcctatg tcaaccgtat caagggttgg acaaatcttc tttagttcgc aatacttgta
    19081 catgaatctg cttaattcgc tccaatagta cgcggtatct cttattcccg tattaataaa
    19141 aaaatgaagc attttgagat ttgcagttgg acttggagcg attacttcat tataaaggtc
    19201 tcttacatcg gtgtatctga taccaagcct ggatgtataa aattcaaatt tgggctcttc
    19261 atctgcggca agccggattc ctaaatcata aggaaccttt atattttcat cgtaaaattt
    19321 gatttcattc aaatcgtcta aaatcggaat gcaattcttg aaaccctcat ttagcagctc
    19381 tgaaatgtat tgacggtaaa gcggtctttt aaatccattg catattacgt aggtatcttt
    19441 ggtaatcttg ccttctttca gaagttttcg cacaatggga atgtcaaacg ccgaagaggt
    19501 ttctatatga acatcatttt tcaatgcttc atcgaaaata aatttgtagt gtgaagattt
    19561 ggtgcaatag ctatacaggt atttcccctt ataattgaat tttgccatgg catctccaaa
    19621 aagcttctga gcaatcttta tgttctcgct gatttttgga aggtaggtga ccttgagggg
    19681 tgtcccatat tccttgatta gcttcatgag agggacatcg ttgaaataga gttcgttgtt
    19741 ttcaaccctg aattccttct gaggaaattc gaaggtttgc tcaatgagat ctatatattg
    19801 cttcattatg tgaaatgcat aaaatggatt ttaaaaggac ctgcaataat ctaataattt
    19861 aacaattaat ccaactaatt gccccaccaa taaacctgga gatggaaatt ctgaaagtct
    19921 ggcaacctgg gtaaaagtaa atcccaaacc ccatagttct tgataaggcc caatcgagta
    19981 aatttgggca taaattctca tttatgaagc ttcaggttcg ggatgaaatt tcgccactcc
    20041 ggtcagttat tgtgggatcg gcttttgatt ttggggggac acctctgtat aaagaagctt
    20101 atgatccgaa atcaaaagag aatattcgaa acggcacttt tccaacggaa gaggatctgt
    20161 tggaggaaat tgatggcttt gcggaaaaat tgaaagccat gggggtttat gtttacaggc
    20221 ctcagaatgt agaaggtatc aaccagatat ttgccaggga tatcggctta gttattgaag
    20281 attttttcat aaaagcgaat atcgtcgccg accggacaaa ggaaataaga gggatattac
    20341 acctgcttaa gaaaattgac tcccgaaaag ttgttcagcc gcctgtgaaa gtccgaattg
    20401 aaggtggaga tgtgatgccc tggtatgata agattttcat cggctattca aagtatgccg
    20461 attttcaaaa atttattgtc tcccgaacca atgaggcagg gatagaattt gttaagcagc
    20521 tgtttcctga taaggaagta attccttttg aacttaataa gtcagataat gatgccagga
    20581 agaatgcact gcatctggac tgctgttttc agccgatagg taaaggtttt gctataatgt
    20641 accgtgatgg ctttaagaat aagcgggaag caaattacct ggttaatttt tttgggcagc
    20701 ccaaaatgat tttcatagac cagcaggaaa tgtatgatat gtgcgcaaat attttttccg
    20761 taaatccgaa tttaataatt tcatcaaaaa attttgttcg tcttaacaag gaactcagag
    20821 atcgtggctt caaagttgaa gaagtaaaat ataatgaaac ggcaaaaatg gaaggacttt
    20881 tgagatgctc aacattacct ttaagaagaa gttgaaattt atgcaaatta cttcgcacat
    20941 attgatgatt agacccgtgc agtttcgtta caatgaacaa accgctgaaa acaacttcta
    21001 tcaaaaggat cctgataatc ttgatgatga aactgcagga gaaaaggcgc agcgtgagtt
    21061 tgacaacttt gtacaggtct tgcgctcaaa aggcgtggat gtgctggtgg tggaagatac
    21121 ttttagttcc gatactcctg attctatttt ccctaataac tgggtctcat ttcatgagga
    21181 tggcacagtt gttttatttc cgatgtttgc ccccaaccgg agaagggaaa gaagaaagga
    21241 tattcttaac gatattttac caggacgtgg ttttgcagtt aagaagataa ttgatttttc
    21301 tgattttgaa aaaagtgaaa gatttttgga agcaaccgga agcatggtaa tggataggga
    21361 taatagaatt atttacgcgt ccatttcaga aagaaccgac cctggccttg ttgatgaatt
    21421 ctgcataaaa tttggataca aggctgtgaa atttcattct tatcaaacct accagggaga
    21481 acggcttccc atttatcata caaatgtaat gatgaccttg gcagatgaat ttgccattat
    21541 ttgcggagat gccgttgata ataaagaaga attgaaagac gttgtctcta atctcaaaaa
    21601 aaccgggaag gagataatct atattactga agagcaaacc aggaaatttg cgggaaatat
    21661 gctccaggta ataaacaaag acggaaagaa actggtggta atgtcttcag cggcctacca
    21721 tagtcttaat cccgaacaag ttcggcaagt gcagcactac ggtgagttgg tacacagtga
    21781 tttgaagacc attgaaacct gcggaggcgg aagtgcaagg tgcatgatgg cggaagtatt
    21841 cctgccaatg gtggattttt ctcttgataa accttcagaa ttgataaatt tgccctcatc
    21901 aaactaatta ttacgtaatg ccagatggac ctgtaacagc ttttattgaa aagcactatt
    21961 tgcactttaa tgccgctgcc cttgttgatg cttccaaagc ctacataaaa catctggaag
    22021 agggtaagaa aatgatgatt accctggcag gagccatgag caccgcggaa ctggggatat
    22081 cactcgcaga aatgatccga caagataaag ttcagatcat tacatgtacc ggagcaaacc
    22141 tggaagaaga tctgatgaat cttgtcgctc ataattccta taaacgcgtt ccgaattaca
    22201 gagatctaac tccaaaagaa gaatgggaac tgcttgaaaa gggttacaat cgggtaacgg
    22261 atacctgcat acctgaggaa gaagccttcc gcagacttca gaaacatatc tttaaaatct
    22321 ggaaagaagc ggaggaaagc ggagaacgtt attttcctca tgaatatatg tttaagatgc
    22381 tgctttccgg cgttttggaa caatattatg aaattgactc aaagaacagc tggatgttag
    22441 cagcagcaga aaagaatttg ccaatcattg ttccgggttg ggaagattct accatgggaa
    22501 acatttttgc ctcctattgt attaagaatg agttgacacc gtctaccatg aagtccggaa
    22561 tagaatatat gacctggttg gctgattact ataccaggaa tgccggaacg gaaggcatag
    22621 gattcttcca gattggcggt ggtattgccg gagattttcc catctgtgtt gtccctatgc
    22681 tttaccagga tcttaaaaga acagaaactc ctttctggag ttatttctgt cagatttcag
    22741 attctactac aagttttggt tcttattccg gcgcggttcc aaatgaaaaa ataacctggg
    22801 gcaaactagg gattgataca cctaagttta tcatagaatc ggatgcctct attgtggcac
    22861 cgctcgtatt tgcctatata ctcggctggt aagatgaaaa ctttgcgaat tgaagatcag
    22921 ataaaagaag ggatcactct ggcctttaat caattattta aagcggaggt aaagtcagag
    22981 gaaataaagt tgcaaccaac caataaagaa tttgaaggcg acttcacatt tgttgttttt
    23041 gcatttctga aagaaacaaa aaaatcaccg gaagaatccg gaaaaatgat tggggaattc
    23101 ataatgaatc acaccggatt gatcgactct tataatgttg taaagggatt tctgaattta
    23161 gtgatttctg gagaagtgtg gatatcaaca ctaaaaaata tcgcaaacaa tgatcagcac
    23221 gggttcaagc aacgtaacgg cacgcaggta atggttgaat actcctcacc caacaccaat
    23281 aagccgcttc acctcggaca cctgcgaaac aatttccttg gatactctgt agcgaaaatt
    23341 cttgaagcaa atggcaatac agttcataaa gttcagatta taaatgacag gggaattcat
    23401 atttgtaaat ctatgcttgc gtggataaaa tttggcaaag gtgaaacacc
//
